bluuebunny/crossref_metadata_2025_split huggingface dataset:
CHROMATOGRAPHIC SEPARATION OF URINARY CORTICOSTEROIDS
Authors: Emberland R. , Støa K. F. 
Published: December 2008

ABSTRACT
The usefulness of partition chromatography on cellulose columns has been tried in fractionation of urinary corticosteroids. As the chromatographic system toluene/propylene glycol has been used. During the first part of the experiments the determination of the corticosteroid content in the chromatographic fractions was carried out by measuring their reducing power against phosphomolybdic acid. Provided the right conditions were used, a satisfactory separation was obtained of the following steroids in mixture: 11-deoxycorticosterone, 11-dehydrocorticosterone, 11-deoxy-17-hydroxycorticosterone, corticosterone, cortisone, dihydrocortisone, tetrahydrocortisone, cortisol and tetrahydrocortisol. Aldosterone appeared in the same fractions as cortisone. By means of the borohydride-bismuthate reaction and a correction by the Allen formula in the subsequent spectrophotometry, an approximatively specific determination of a series of 17-hydroxycorticosteroids in urine extracts could be obtained.
A COMPARISON OF SEMINAL VESICLE, KIDNEY AND ADRENAL RESPONSES IN THE RAT FOLLOWING THE ADMINISTRATION OF ANDROGEN
Authors: Grunt Jerome A. , Walker Joseph E. 
Published: December 2008

ABSTRACT
The responses of several seminal vesicle in vivo microscopic characteristics, seminal vesicle contractions, seminal vesicle, kidney and adrenal weights and seminal vesicle histology have been compared following the administration of testosterone propionate (T. P.) and norethandrolone (19-NT) to castrated male rats.
Fourteen days after castration 35 male Osborne-Mendel rats were divided into 5 groups each containing 6 to 8 animals and given daily subcutaneous injections of 0.1 ml sesame oil with or without added steroids. The groups were: I – sesame oil; II – 100 μg 19-NT; III – 500 μg 19-NT; IV – 100 μg T.P.; V – 500 μg T. P.
After 7 days of injections, seminal vesicles were studied by the transillumination method of Knisely. The seminal vesicles, adrenals and kidneys were then removed and weighed and the vesicles were prepared for routine histological examination.
The adrenal and kidney weights, as well as the seminal vesicle contractions, responded to both steroids in a similar manner, however, seminal vesicle weight and histology and in vivo characteristics differed greatly. 19-NT appeared to have very little effect on vesicle weight, in vivo characteristics and histology although contractions were inhibited in 13 of 14 animals so treated. Therefore, when used as the end-point in hormonal reactions, seminal vesicle contractions should not be considered comparable to the usual secondary sexual organ reactivity to hormones.
THE LEVEL OF URINARY NEUTRAL 17-KETOSTEROIDS IN FEMALE RATS
Authors: Kullander Stig 
Published: December 2008

ABSTRACT
The excretion of 17-ketosteroids in the urine was studied in female rats aged 6 months, 1 year and 2 years. Some of the animals were intact, some were spayed at 3 weeks whilst in some of the spayed animals, ovarian tissue was autotransplanted to the spleen at the time of the operation (ovarian tumours form in this ovarian tissue after 6 months to 1 year).
Normal female rats excreted about 30 μg neutral 17-ketosteroids in the urine per day independently of age (6 months to 2 years). The adrenal weight was smaller in spayed, female rats and the excretion of neutral 17-ketosteroids was less than in intact animals. Spayed female rats with ovarian tumours in the spleen excreted relatively large amount of 17-ketosteroids, the amount appearing to vary with the size of the ovarian tumours in the spleen.
DER EINFLUSS VON THYREOTROPEM HORMON AUF DAS ELEKTROGRAMM DER SCHILDDRÜSE
Authors: Krüskemper H. L. , Reichertz P. 
Published: December 2008

ABSTRACT
The elcctrogram of the thyroid gland in normal guinea pigs shows a lasting monophasic reaction beginning 1–2 minutes after the intravenous injection of thyrotrophin (TSH). With a dose above 1.6 IU of TSH no relation between reaction and dose has been observed. Distinct changes of the electrocardiogram may be observed after intravenous injection of more than 12.5 IU of TSH. Probably they are due to the presence of non-specific proteins. The early reaction in the electrogram of the thyroid gland after injection of TSH is interpreted as a consequence of the stimulated secretion of thyroid hormones.
ZUR PATHOGENESE DES PSEUDOHYPOPARATHYREOIDISMUS
Authors: Schwarz Gerhard 
Published: December 2008

ABSTRACT
A case of pseudohypoparathyroidism with positive Ellsworth-Howard-test is reported. In spite of this result is was possible to confirm by phosphate clearance Albright's hypothesis of non-responding target organ; for parathyroid-extract augmented only phosphate-filtration but not the reabsorption of phosphate, as it is to be expected in hypoparathyroidism. As a clinical feature there is a considerable polyarthrosis.
EFFECT OF RESERPINE, 5-HYDROXYTRYPTAMINE, AND POLYMYXIN B ON MAST CELLS AND TENSILE STRENGTH OF HEALING WOUNDS
Authors: Fiore-Donati Luciano , Moltke Erik 
Published: December 2008

ABSTRACT
The effect of reserpine, 5-hydroxytryptamine (5-HT), polymyxin B and fasting on the mast cells and tensile strength of healing wounds has been investigated.
Reserpine and polymyxin B were given during the last three days before the wounds were inflicted, while 5-HT was given as daily injections after the incisions. Reserpine and – to a lesser extent – 5-HT caused a significant decrease in the tensile strength of the wounds. This decrease was probably not caused by the concomitant weight-loss since a group of fasted animals which showed the same weight-loss turned out to have a normal tensile strength. The animals treated with polymyxin B showed no significant decrease in tensile strength. Treatment with reserpine and 5-HT was not followed by any significant changes in the mast cell morphology, while polymyxin B caused marked changes, the nature of which could not be determined.
It is concluded that the decrease in tensile strength of healing wounds induced by 5-HT and reserpine is probably not mediated by the tissue mast cell.
PATHOLOGICAL ANATOMICAL FINDINGS IN THE ADRENAL CORTEX IN TWO CASES OF CONGENITAL ADRENOCORTICAL HYPERPLASIA
Authors: Seelen J. C. 
Published: December 2008

ABSTRACT
The pathological anatomical findings in the adrenal cortex of two patients, aged 4 and 6, have been studied.
Both adrenal glands had a cortex similar to that seen shortly after puberty; the reticular zone, however, was wide even for the postpuberal age. These findings are definitely anomalous for children who normally have a very poorly developed reticular zone. Hence a marked hyperplasia of this zone was present.
Unlike other investigators, we found a normally developed fascicular zone with a normal foamy cytoplasm; the lipid distribution and double refraction also revealed a normal aspect.
It is concluded that hyperplasia of the reticular zone is the characteristic feature of congenital adrenocortical hyperplasia and that the anomalies in cortisol synthesis are not clearly manifested in the fascicular zone.
WATER AND ELECTROLYTE COMPOSITION OF DECIDUAL TISSUE OF THE RAT UTERUS
Authors: Bitman Joel , Wrenn T. Randall , Cecil Helene C. , Sykes J. F. 
Published: December 2008

ABSTRACT
Studies were made of H2O, Na, K, and Cl in rat uteri with and without deciduoma and in uteri from ovariectomized rats. Samples of whole uterus, endometrium and myometrium were obtained. The proportion of endometrium increased from 6.6 % to 27.2 % when deciduoma were present. Uterine horn weight increased from about 300 mg to over 900 mg. With the development of deciduoma the following effects occurred in whole uterus and myometrium:
H2O content increased about 3.5 %. Na and Cl increased 22 % on a dry weight basis, although there were no differences when based upon wet weight. K increased 16 % on a wet weight and 40 % on a dry weight basis.
The water and electrolyte content of uteri from ovariectomized rats was very similar to that of uteri without deciduoma. Endometrium could only be obtained from uteri with decidual tissue. H2O, Na, and Cl were about equal to the concentrations in whole uterus with deciduoma, but K was considerably higher than that observed in whole uterus. The ionic changes which the rat uterus exhibits during the development of deciduoma are characteristic of tissue growth. Similar changes have been observed in uterine growth under the influence of progesterone in the rabbit and the cow.
STUDIES ON HAEMORRHAGIC LIPAEMIA IN RATS WITH PARTICULAR REFERENCES TO THE EFFECT OF HYPOPHYSECTOMY
Authors: Punsar Sven , Härtel Gottfried , Louhija Antti , Heinonen Olli P. 
Published: December 2008

ABSTRACT
Repeated bleeding in rats caused a definite increase in the serum cholesterol and phospholipid concentrations and a visible lipaemia. The development of lipaemia could be inhibited by injections of red cells in amounts sufficient to prevent the anaemia. The injection of serum corresponding in amount to that withdrawn had no effect in this respect. In hypophysectomized rats, the bleeding caused a similar increase in the serum cholesterol and phospholipid levels but no visible lipaemia.
OESTROGEN ANTAGONISMS: TEMPORAL MODALITIES IN STEROID BLOCK OF OESTRONE-INDUCED CHANGES IN VAGINAL SMEARS OF MICE
Authors: Edgren Richard A. , Neer Helene D. 
Published: December 2008

ABSTRACT
In an earlier paper simultaneous testosterone propionate (TP) was shown to inhibit the vaginal smear effects of oestrone given over two days to spayed female mice. Deoxycorticosterone acetate (DCA) and progesterone were inactive in that test. Oestrone was given at a dose of 2 μg and the other steroid at 1000 μg. If these same doses were given over a four day period, however, all three steroids blocked the oestrone-induced changes. In a series of tests in which the oestrone was given over four days and the blocker either in one or two day periods, TP was found to antagonize when administered with the early but not with the late oestrone injections. DCA was an inhibitor only when given with the late oestrone administrations.
The period of progesterone activity was midway between that for TP and that for DCA, and overlapped each. These patterns of response were best discriminated when the blocker was administered in a single injection.
THYROIDAL ACTIVITY IN HIBERNATING CHIROPTERA
Authors: Sadler Walter W. , Tyler Walter S. 
Published: December 2008

ABSTRACT
Hibernation was induced in groups of the bats Antrozous pallidus (family Vespertilionidae) and Tadarida mexicana (family Molassidae) by holding them for various periods of time in an ambient temperature of 4° C while control groups were held at 22° C and since the bat Macrotus californicus will not hibernate, groups of these were held at 22° C and 37° C to test for differences in thyroid activity as measured by its uptake of 131I over a 24 hour period. It was found that, in contradistinction to reports on other species, the thyroid of Antrozous and Tadarida bats is active during periods of low metabolic levels encountered in hibernation, as evidenced by its uptake of iodine, although the log uptake shows a linear regression with time in hibernation. Season, sex, sexual activity, species, ambient temperature, and length of time in a given ambient temperature were each found to be responsible for significant differences in the percentage uptake of 131I. The activity of the thyroid of the Macrotus bats was found not to be responsive to changes in ambient temperature over the range employed.
THYROIDAL ACTIVITY IN HIBERNATING CHIROPTERA
Authors: Sadler Walter W. , Tyler Walter S. 
Published: December 2008

ABSTRACT
Quantitative and qualitative chromatographic analyses were made of the radioiodinated compounds found in the thyroids of Antrozous pallidus 24 hours after injection of 131I into a group held at 22° C and into other groups held for varying periods at 4° C. The results indicate that the thyroid of the hibernating bat is still active in the synthesis of iodinated amino acids through the sixth day at an ambient temperature of 4° C. The quantity of each compound regresses with time in hibernation, as does the uptake of iodine. Among the compounds containing organically bound 131I, diiodotyrosine becomes progressively less prominent while monoiodotyrosine and thyroxine become progressively more prominent with time in hibernation.
UNTERSUCHUNGEN ÜBER DIE EXTRAKTION DES ROTEN KOBER-FARBSTOFFS DURCH ORGANISCHE LÖSUNGSMITTEL ZUR ÖSTROGENBESTIMMUNG IM HARN
Authors: Ittrich Gerd 
Published: December 2008

ABSTRACT
A series of organic solvents and phenol derivatives have been examined for the extraction of the pink Kober-colour complex. Optimal results could be achieved for fluorimetry by a solution of 2 % (w/v) p-nitrophenol and 1 % (v/v) ethanol in acetylenetetrabromide, when the green mercury line (546 mμ was used as primary light.
The sensitivity, stability and specificity have been improved, compared with the previously described reaction.
By changing the sequence of purification steps and by reducing the volume of the urine sample (5 ml) the method for the determination of total oestrogens has been simplified. Approximately 10 determinations can be done within 3–4 hours by one person.
Recovery experiments and comparative determinations with a previously described method have been carried out.
The excretion of total oestrogens in a complete menstrual cycle is determined with the described method.
PROGESTATIONAL ACTIVITY OF STEREOISOMERIC PROGESTERONE-ANALOGUES FOLLOWING ORAL ADMINISTRATION IN AMENORRHOEA
Authors: Tillinger K.-G. , Diczfalusy E. 
Published: December 2008

ABSTRACT
A stereoisomeric analogue of progesterone, 9β,10α-pregn-4-ene-3,20-dione (retro-progesterone) and a 6-dehydro derivative, 9β,10α-pregna-4,6-diene-3,20-dione (6-dehydro-retro-progesterone) were studied for their progestational activity in 26 artificial cycles induced in 17 amenorrhoic women.1 It was found that 6-dehydro-retro-progesterone is a highly potent orally active progestational agent in the human subject. Daily doses of 10 mg of this compound given orally for 10 days induced a definite secretory transformation of the endometrium. The histological appearance of the endometrium following treatment with this compound could not be distinguished from that seen in normal cycles. Both compounds studied were excellently tolerated; no toxic side-effects were observed.
THE EFFECT OF NORETHISTERONE ON ANTERIOR PITUITARY FUNCTION, STEROID METABOLISM AND BASAL TEMPERATURE IN MALES
Authors: Parada J. , Napp J. H. , Voigt K. D. 
Published: December 2008

ABSTRACT
The effects of 200 or 300 mg norethisterone oenanthate i. m. or of 20 mg norethisterone daily by mouth for 16 days on anterior pituitary function, steroid metabolism and on basal body temperature were investigated in five males without any demonstrable endocrine disturbance.
During a 7 day pre-treatment and a 14 day treatment period, the behaviour of basal body temperature, of oestrogens, Porter-Silber chromogen (PSC) and gonadotrophin excretion, was studied. In addition, an intravenous loading dose of corticotrophin was given once in the pretreatment and once in the treatment period. No definite effect on gonadotrophin and oestrogen excretion was established. Values of total PSC were practically identical, but the average value of free PSC in urine during the treatment period was significantly higher. An intravenous loading dose of corticotrophin also showed a significantly higher rise in plasma free PSC at this period.
The findings are briefly discussed with reference to the literature.
NOUVELLES DONNÉES SUR LE POIDS MOLÉCULAIRE DE LA GONADOTROPHINE DU SÉRUM DE JUMENT GRAVIDE
Authors: Bourrillon Roland , Got René 
Published: December 2008

ABSTRACT
Sedimentation constant of pregnant mares' serum gonadotrophin remains equal to 3.7 in water, phosphate buffer pH 7.3 and ClNa 0.1 m. But, diffusion constant, equal to 10.2 in water, becomes 4.2 in ClNa 0.1 m. Then, the molecular weight gets 68 500 instead of 28 000 in water.
AN IMPROVED METHOD FOR THE ASSAY OF ANTI-INFLAMMATORY SUBSTANCES IN RATS
Authors: Bush I. E. , Alexander R. W. 
Published: December 2008

ABSTRACT
An improved method for assaying anti-inflammatory substances is described. Granulomata are produced by means of cotton-wool pellets which have been impregnated with carrageenin. The precision of the assay when used for anti-inflammatory steroids is approximately double that obtained with plain cotton-wool pellets.
IMPAIRED WATER EXCRETION CAPACITY IN PRIMARY MYXOEDEMA IMPROVED BY CORTICOSTEROIDS, CORTICOTROPHIN AND THYROID SUBSTITUTION
Authors: Vogt Jörgen Herman 
Published: December 2008

ABSTRACT
In four out of twelve cases of primary myxoedema, water excretion after a water load was found to be impaired. In two cases the duration of myxoedema was less than a year. Thyroid substitution was given for more than a year in two of the cases before improvement of water excretion capacity was obtained. During thyroid substitution but before improvement of water excretion was obtained, cortisone in small doses improved water excretion.
17-ketosteroid excretion was low in all 4 patients and 17-ketogenic steroid excretion was normal, as was the response to corticotrophin (ACTH) except in the fourth patient, where the response to ACTH was subnormal even after 10 months of thyroid substitution and improved but was not completely normal after 16 months. In this case too improvement of water excretion by thyroid substitution was obtained, but only after 16 months of such therapy. Cortisone and ACTH corrected the faulty water excretion prior to correction by thyroid substitution as did prednisolone, but not aldosterone.
Disagreement is expressed with the interpretation given by Crispell et al. (1954) of their findings in similar cases.
The impaired water excretion is explained on the assumption of a sluggish release of ACTH in some cases of myxoedema in response to a water load. One of the patients recorded had a history of previous hepatitis and kidney infection, and definite but not serious liver damage. In this case liver function was definitely worse in the myxoedematous state, thyroid substitution slowly bringing about a considerable improvement.
Plasma creatinine was increased in two patients when myxoedematous but normalized after long-lasting thyroid substitution.
The improvement of hepatic function and renal glomerular filtration obtained during thyroid substitution is not considered to be the cause of the improved handling of a water load.
In cases in which there is strong suspicion of impaired adrenocortical function, whether primary or secondary, intravenous ACTH infusion may cause an almost fatal shock. Intramuscular ACTH is well tolerated. Aldosterone treatment was tried in one of the patients suffering such shock.
ADRENALECTOMY DURING PREGNANCY WITH THE APPEARANCE OF PRE-ECLAMPSIA AT TERM IN A CASE OF CUSHING'S SYNDROME
Authors: Bergman Per , Ekman Hans , Håkansson Britt , Sjögren Björn 
Published: December 2008

ABSTRACT
This is a case report of a woman, suffering from Cushing's disease, who conceived before treatment and who underwent successful adrenalectomy during pregnancy. The gestation continued normally until near term when symptoms of pre-eclampsia appeared on unchanged cortisone substitution. The urinary excretion of pregnantriol during the last trimester of pregnancy is described.
ZUR SUBMIKROSKOPISCHEN STRUKTUR DER RATTENSCHILDDRÜSE ELEKTRONENMIKROSKOPISCHE UNTERSUCHUNGEN AN DER BASIS DES SCHILDDRÜSENFOLLIKELS
Authors: Waller U. 
Published: December 2008

ABSTRACT
This is a description of ultramicroscopic structures at the base of the thyroid follicle. The structural principle of the subendothelial or interfollicular space, namely basement membranes closely following both the endothelial cells of the capillaries and the epithelial cells of the thyroid, corresponds to that described by other authors in the other endocrine organs. Especially shown are small vesicle like decreases of intensity in the subendothelial space, causing bulges in both the subepithelial basement membrane and the membrane covering the endothelial cell pores thereby forming pseudopores. This membrane is in several parts a double one. The possible role played by the above mentioned structures in the basal secretion of the thyroid epithelial cell is discussed.
MODE OF ACTION OF IODIDE AND IODIZED COMPOUNDS ON THE THYROID AS JUDGED FROM THE BEHAVIOUR OF THE PBI AND THE 131I TRACER TEST. HYPOTHESIS OF CERTAIN PHASES OF THE THYROIDAL FUNCTION
Authors: Thorén Arne 
Published: December 2008

ABSTRACT
The thyroid is assumed to contain a substance which forms, with iodide, iodine and iodine compounds, a freely dissociable complex, whereby it is able to concentrate and retain them. The »sites« afforded by this substance are not specific for iodine, since thiocyanate, perchlorate, and certain other anions become attached to them. The stability of attachment varies with different substances and thus the »blocking« actions of thiocyanate, perchlorate, iodized compounds, and diagnostic agents differ in their duration of action. Iodide, iodine, and intermediate compounds in the synthesis of thyroid hormone are normally retained at these sites. This theory accounts for the effects of thyrotrophin, iodide, thiocyanate, and perchlorate on the concentrating power and organic binding in the thyroid; and for the effects of iodide, iodized agents and antihyroid compounds on PBI and tracer tests, as well as for the effect of iodide in thyrotoxicosis, and of intravenously administered iodide in thyrotoxic crisis.
TURNER-SYNDROM MIT VOLL AUSGEBILDETEN SEKUNDÄREN GESCHLECHTSMERKMALEN UND FERTILITÄT
Authors: Bahner F. , Schwarz G. , Hienz H. A. , Walter K. 
Published: December 2008

ABSTRACT
Two women with normally developed secondary sex characteristics are reported. Both had spontaneous menstrual cycles, the first one during a period of 4 years, the second one started menstrual bleedings at the age of 17 and had menstrual cycles of regular intervals up to the present age of 39. She had given birth to a healthy boy at the age of 31. Both patients are chromatinnegative, of short stature and one has a unilateral webbed neck. Therefore they had to be classified as cases of Turner's syndrome. In cultures of bone marrow and skin fibroblasts the patient who has born a child was shown to have 45 chromosomes (2n + OX). The findings presented are in contrast to latest hypothesis of chromatinnegative Turner's syndrome according to which sex chromosomal anomaly XO leads to development of rudimentary ovaries.
EFFECT OF NORETHANDROLONE ON THE URINARY EXCRETION OF 17-KETOSTEROIDS, TOTAL 17-HYDROXYCORTICOSTEROIDS AND UROPEPSIN AND ON THE GASTRIC SECRETORY ACTIVITY
Authors: Castrén O. , Kalliomäki L. , Pekkarinen A. , Soiva K. , Viikari S. 
Published: December 2008

ABSTRACT
The effect of orally administered 17α-ethyl-17β-hydroxy-19-norandrost-4-en-3-one (75 mg daily during 8 days) on the urinary excretion of 17-ketosteroids, total 17-hydroxycorticosteroids and uropepsin and on the gastric secretory activity has been studied in 10 male patients with peptic ulcers. The excretion of 17-ketosteroids was significantly reduced, but the excretion of total 17-hydroxycorticosteroids and uropepsin and gastric secretory activity were not affected by the treatment.
CELLULOSE COLUMN CHROMATOGRAPHY OF OVARIAN PREPARATIONS CONTAINING UTERINE-RELAXING ACTIVITY
Authors: Paul K. G. , Wiqvist N. 
Published: December 2008

ABSTRACT
Chromatography of extracts from pregnant sows' ovaries on carboxymethyl cellulose columns distributes the material into three components, the second and third of which contain uterine-relaxing activity. Heating of the second component makes the activity migrate into the position of the third component.