bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoplastic transformation induced by furylfuramide and nitromethylfuran of embryonic hamster cells in tissue culture
Authors: Nishi Yoshisuke , Taketomi Masako , Inui Naomichi 
Published: February 2007

AbstractSecondary cultures of Syrian hamster embryonic fibroblasts were tested for transformation and neoplastic properties after exposure in vitro to furylfuramide (AF‐2) and other nitrofurans. Typical morphological transformation was seen in five of six cultures between 30 and 186 days following treatment with 5–10 × 106 M AF‐2 for 24 h. Transformation was seen in only one of four cultures 145 days after treatment for 6 h with AF‐2. Treatment with 5–10 × 10−6 M NMF (5‐nitro‐2‐methylfuran) for 24 h also induced transformation after 50 and 118 days in two cultures. In contrast, untreated cultures, and cultures treated with 5–10 × 10−6 M NFT [4‐(5‐nitro‐2‐furyl)thiazole] for 24 h were not transformed within 200 days. Three of the six lines transformed by AF‐2 and both lines transformed by NMF also became tumorigenic 7–24 days after morphologic transformation. The other three transformed lines produced nodules which regressed within a few weeks of transplantation. Untreated and treated non‐transformed lines did not produce tumors during an observation period of 6 months. The tumors were classified as fibrosarcomas. The ability to form colonies in soft agar was acquired by only one tumorigenic line.
In vivo and in vitro TSTA‐inducing activity of temperature‐sensitive (TS) mutants of SV40
Authors: Deichman G. I. , Kluchareva T. E. , Kashkina L. M. 
Published: February 2007

AbstractIn vivo TSTA induction in Syrian hamsters was studied with the use of SV40 ts mutants (A, B, C, BC and D). The ts A30, ts A239 and possibly also the ts BC210 mutants were defective in resistance‐inducing activity in hamsters in contrast to wild type SV40 and other ts mutants. At the permissive temperature ts A30 and ts A239 did not induce TSTA in hamster cells during abortive infection in vitro, while they did so in green monkey cells at both permissive and non‐permissive temperatures. In hamster cells transformed by ts A30, ts A239 and ts A209 mutants none at all or very little TSTA was detected by in vivo transplantation immunological tests. Thus, expression of TSTA induced by these three SV40 ts A mutants was found to be dependent from the species of infected cells and was being a temperature independent viral function.
Association of human hepatocellular carcinoma and cirrhosis with hepatitis b virus surface and core antigens in the liver
Authors: Nayak N. C. , Dhar Asha , Sachdeva Rajeev , Mittal Asha , Seth H. N. , Sudarsanam Doss , Reddy B. , Wagholikar U. L. , Reddy C. R. R. M. 
Published: February 2007

AbstractParaffin sections of livers obtained at autopsy from 50 cases of hepatocellular carcinoma (HCC), 58 cases of cirrhosis and 54 cases of other miscellaneous liver disorders (controls) were stained for both surface (HBsAg) and core (HBcAg) components of hepatitis B virus (HBV) by immunoperoxidase and immunofluorescence techniques and rigidly controlled for antigen specificity, and in addition stained by orcein for HBsAg. The material was collected from different regions of India and adequate amounts of tissue were examined in most specimens to overcome possible sampling error caused by random distributions of the antigens in liver. HBsAg was detected in 94% of HCC, 71% of cirrhosis and only 2% of control livers, while HBcAg was found in 22%, 31% and none respectively. Antigen positivity seems to be directly related to the amount of tissue examined. Peroxidase staining detected smaller amounts of HBcAg than fluorescence and was also much more convenient for identifying the antigen. Both antigens were present in 9 of 41 HCC cases, 12 of 39 cirrhosis and none of 25 controls. Most of these livers contained 1+ HBsAg and 1+ to 2+ HBcAg, an antigen expression pattern suggestive of a carrier state or, rarely, of mild chronic liver disease. Among all livers tested, HBsAg alone was present in 48, both antigens were found in 21, and HBcAg alone in none. HBsAg was seen inside tumour cells in four cases, but no tumour showed HBcAg. Most HCC was associated with cirrhosis (92%) and antigen‐positive cirrhosis had a higher chance of harbouring HCC than antigen‐negative disease. HBsAg was detected in all four non‐cirrhotic livers associated with HCC, while two of these also had HBcAg. Active cirrhosis was very frequently associated with HBsAg. These results and the overwhelming evidence of sero‐logical and epidemiological studies from various parts of the world suggest a strong association of the hepatitis B virus with HCC. The possible ways in which the two could be related are discussed.
Isolation of infectious EB virus from the epithelial tumour cells of nasopharyngeal carcinoma
Authors: Trumper P. A. , Epstein M. A. , Giovanella B. C. , Finerty S. 
Published: February 2007

AbstractEvidence of herpesvirus replication has been found by light and electron microscopy in the malignant epithelial cells of two out of six naso‐pharyngeal carcinomas (NPC) examined directly after growth in nude mice to eliminate non‐malignant infiltrating cells. The agent has been identified as EB virus by immunofluorescence tests for EB virus capsid antigen, and has been shown to be biologically active by its ability to infect and transform foetal cord blood lymphocytes. Lympho‐blastoid cell lines which express the EB virus nuclear antigen have been established from the transformed foetal lymphocytes, and thus carry the first isolate of the virus from the actual epithelial tumour cells of NPC, in a form suitable for further investigation. The results are discussed in terms of the relationship of EB virus to NPC epithelial cells.
Nasopharyngeal carcinoma: Significance of changes in epstein‐barr virus‐related antibody patterns following therapy
Authors: Henle Werner , Ho J. H. C. , Henle Gertrude , Chau J. C. W. , Kwan H. C. 
Published: February 2007

AbstractSera were collected at intervals of 3 to 8 months over a 4‐ to 5‐year period from more than 103 patients with nasopharyngeal carcinomas (NPC) and examined for their spectra and titers of antibodies to Epstein‐Barr virus (EBV)‐related antigens. They were titrated for IgG, IgA and IgM antibodies to EB viral capsid antigen (VCA), for IgG and IgA antibodies to the D (diffuse) and R (restricted) components of the EBV‐determined early antigen (EA) complex, and for antibodies to the EBV‐associated nuclear antigen (EBNA). In the as yet untreated patients the incidences and titers of most of the antibodies increased with the stage of the disease; i.e., essentially with the total tumor burden. Such increases were observed in individual patients whose disease ultimately progressed to death, at times well in advance of the recognition of relapses or metastases. Increases were not noted or were only minor and delayed in some fatal cases if the tumor extended to the cranial cavity in the absence of significant involvement of cervical lymph nodes. In contrast, patients who responded well to therapy and remained clinically free of disease during the 4‐ or 5‐year observation period after, at most, early minor relapses, showed gradual, steady declines in the titers of all antibodies except anti‐EBNA. Thus, VCA‐specific IgA and D‐specific IgA and IgG became undetectable in time in many of the patients. In several long‐term survivors the declines were arrested at given levels or a reversal to increasing titers was noted which was followed in time by detection of a recurrent tumor or metastases in some cases, but not in others who remain under close observation. It would appear that serologic monitoring of NPC patients may warn of recurrent tumor activity well before it becomes clinically evident.
Characterization of immunoglobulins in Hodgkin cells
Authors: Landaas Tone ø. , Godal Tore , Halvorsen Tore B. 
Published: February 2007

AbstractFifteen of 42 formalin‐fixed lymph‐node biopsies with Hodgkin lesions of different histological types and stages gave positive staining for immunoglobulins by the peroxidase/antiperoxidase method. The immunoglobulins in all the positively stained Hodgkin cells were of IgG type, but staining for IgA, D and E was negative. One of the 15 biopsies was positive for IgM. Thirteen of the 15 positive biopsies were positive for both χ and λ. Sequential double staining using two different substrates for peroxidase showed that most of the Hodgkin cells simultaneously contained light chains of both χ and λ type. It is concluded that Hodgkin cells do not contain a “monoclonal” immunoglobulin product.
Glycopeptides from epithelial cell mutants: Temperature sensitive for the transformation phenotype
Authors: Pietropaolo Concetta , Yamaguchi Nobuo , Weinstein I. Bernard , Click Mary Catherine 
Published: February 2007

AbstractFucose‐labelled glycopeptides obtained from the cell surfaces of normal and transformed epithelial cells were compared by co‐chromatography on Sephadex G‐5O. The material obtained from rat epithelial cells transformed in vitro or from hepatoma cells in culture elutes earlier than the fucosecontaining glycopeptides obtained from normal rat epithelial cells. A mutant (TS 223) of a transformed epithelial cell that is temperaturesensitive for maintenance of the transformed phenotype, varies in its Sephadex G‐50 profile of cell surface glycopeptides when grown at the permissive (34° C) or the non‐permissive temperature (40° C). When grown and labelled at 36° C the gel filtration profile of the glycopeptides resembles that of transformed cells. At 40° C there is an enrichment of later eluting glycopeptides. These differences are more striking in confluent‐phase cultures than in log‐phase cultures. The differences are reversible following upward or downward shifts in growth temperature although there appears to be a lag of at least 6 h before the alteration can be demonstrated by these procedures.
Feline malignant mammary tumors. III. Presence of C‐particles and intracisternal A‐particles and their relationship with feline leukemia virus antigens and RD‐114 virus antigens
Authors: Calafat Jero , Weijer Kees , Daams Henny 
Published: February 2007

AbstractThirty‐six feline mammary tumors were examined by the electron microscope, and by the indirect immunofluorescence (IFA) test with anti‐FeLV and anti‐RD‐114 serum. In 11 (30.6%) tumors intracisternal A‐particles (IAP) were found. One of these tumors contained a few particles with an electron‐dense nucleoid in the cisternae of the endoplasmic reticulum. In 7 (19.4%) other tumors C‐particles were found and in the remaining 18 (50.0%) no particles at all could be detected. In 11 (30.6%) tumors FeLV antigens and in 20 (55.5%) tumors RD‐114 virus antigens were present. In 9 (25%) tumors we found a high (>1/64) and in 11 (30.6%) tumors a low (>1/16‐≤1/64) titer. There was a good correlation between the presence of C‐particles and the demonstration of FeLV‐antigen but none between IAP and FeLV antigens. No correlation was found between RD‐114 virus antigens and any type of particle. Morphologically, the IAP found in feline mammary tumors were indistinguishable from the IAP present in mammary tumors of some inbred mice strains. The IAP in feline mammary tumors possibly represent an endogenous virus, different from RD‐114 virus. The role of these viruses in the etiology of feline mammary tumors is discussed.
Chromosome banding, isoenzyme studies and determination of epstein‐barr virus DNA content on human burkitt lymphoma/mouse hybrids
Authors: Spira Jack , Povey Susanne , Wiener Francis , Klein George , Andersson‐Anvret Maria 
Published: February 2007

AbstractFour independently fused hybrid clones derived from a cross between the mouse mammary carcinoma TA3Ha and the human Burkitt lymphoma line Daudi were tested for the EBV‐determined nuclear antigen (EBNA), EBV‐DNA and the presence of human chromosomes, in the course of serial propagation in vitro. EBNA and EBV‐DNA were lost in parallel with the loss of human chromosomes. It seems that the persistence of EB‐viral genetic information does not require the presence of a specific human chromosome(s) in this particular hybrid combination.
Risk factors for lung cancer in singapore chinese, a population with high female incidence rates
Authors: MacLennan R. , da Costa J. , Day N. E. , Law C. H. , Ng Y. K. , Shanmugaratnam K. 
Published: February 2007

AbstractThe high incidence of lung cancer in Chinese females in Singapore, especially among those belonging to the Cantonese dialect group, and the relatively high rates in Chinese males have been studied by means of interviews of cases and controls. A significant dose‐response effect of cigarette smoking was found for all male and female groups, but neither smoking nor any other exposure explains the high incidence of lung cancer observed in Cantonese females who exhibit high rates of adenocarcinoma apparently unrelated to smoking. In general, persons with a low consumption of green vegetables were at higher risk for lung cancer. This finding might be due to an increased susceptibility in the presence of a relative deficiency of vitamin A.
Further evidence for a non‐random chromosomal abnormality in acute promyelocytic leukemia
Authors: Rowley Janet D. , Golomb Harvey M. , Vardiman James , Fukuhara Shirou , Dougherty Charlotte , Potter David 
Published: February 2007

AbstractWe have previously reported on two patients with acute promyelocytic leukemia (APL) who had what appeared to be a deletion of chromosome No. 17. We now describe a third patient with APL. All three patients had a structural rearrangement involving No. 15 and No. 17. Our current interpretation of the chromosomal abnormality is that it is a reciprocal translocation, t (15; 17) (q22; q21). Evidence that this is a consistent rearrangement associated with APL comes not only from our three patients, but also from two other published cases of APL, studied with banding, who also had an identical abnormality.
EBV specific secretory IgA in saliva of NPC patients. Presence of secretory piece in epithelial malignant cells
Authors: Desgranges C. , Li J. Y. , De‐Thé G. 
Published: February 2007

AbstractSaliva samples from 59 patients with nasopharyngeal carcinoma (NPC) and from 20 normal individuals were studied to determine the nature and origin of the EBV‐specific IgA in NPC saliva. About 50% of NPC saliva samples contained secretory IgA specific for EBV. The corresponding tumor IgA(α) was found in plasma cells surrounding the epithelial tumor cells and the secretory piece at the surface of epithelial cells. A slightly higher proportion of NPC saliva samples containing IgA was found in patients from Tunis than in samples from Hong Kong. Attention is drawn to the clinical value of the salivary IgA in diagnosis and monitoring of treatment of NPC.
Circulating immune complexes in patients with malignant lymphomas and solid tumors
Authors: Heier Hans Erik , Carpentier Nicole , Lange Ghislaine , Lambert Paul Henri , Godal Tore 
Published: February 2007

AbstractSera from 50 patients with Hodgkin's disease, 78 patients with non‐Hodgkin non‐leukemic malignant lymphomas, and 75 patients with different types of solid malignant tumors were investigated for the presence of immune complexes using the (125I) C1q‐binding test. All patients were untreated. An increased serum C1q‐binding activity was found in 22% of the patients with Hodgkin's disease, 35.9% of the non‐Hodgkin lymphoma patients and in 37.3% of the patients with solid tumors. The C1q‐binding material detected in the patients' sera had properties similar to those of immune complexes. On sucrose density gradient it sedimented as a 10‐30 s material. It contained IgG which were dissociated under acid conditions. Passage through anti‐IgG immunoabsorbent removed its C1q‐binding properties. A prevalent association was found between the presence of serum immune complexes and disseminated disease stages in all the patient groups included. A similar association was found between the presence of serum immune complexes and general symptoms among the malignant lymphoma patients. The nature of the antigens involved in the complexes remains unknown.
A microchemical analysis of alkaline phosphatase in human malignant and benign breast tumours
Authors: Larner Elizabeth H. , Rutherford Charles L. 
Published: February 2007

AbstractA microchemical technique has been adapted to the evaluation of the membrane‐bound enzyme alkaline phosphatase within human malignant and benign breast lesions. Frozen tissue sections were either stained or lyophilized in alternating sequence in order that samples for enzyme analysis could be chosen from well‐defined regions of epithelial proliferation. Microgram quantities of human tumors could then be examined with multiple replications to elucidate patterns of overall enzyme activity, the activity of tissue fractions, and the isoenzyme form present within individual tumors. A significant difference (p<0.001) was found to exist between the specific activity of alkaline phosphatase from human infiltrating ductal carcinomas and human fibroadenomas. The activity value determined for the malignant group of tumors was 2.12±1.80, while that for benign tumors was 7.52±4.31 mmoles/min/kg dry weight at 37° C. The percentage of alkaline phosphatase activity found within 29,000 g pellets of malignant tumors was variable and constituted approximately 60% of the total activity found in all fractions, while that for benign tumors was more constant and amounted to approximately 90% of the total found. The malignant and benign tumor alkaline phosphatase of both supernatant and pellet fractions was shown to be 90% labile to incubation at 65° C for 5 min and to be 50% inhibited by 20 mM 1‐phenylalanine. Polyacrylamide gel electrophoresis revealed a single enzyme band of identical Rf for each of these fractions from the two tumor types. When varying proportions of malignant and benign tissue samples were co‐incubated, no influence of tumor type on the total activity or the percentage membrane‐bound activity was found. However, a change in the proportion of enzyme activity pelleted at 29,000 g could be shown when the cellularity of the tissue sample was varied. While homogenization of the total sample or of the insoluble material could release some of this activity, greater enzyme activities were always found within insoluble, 480 g pellets and 29,000 g pellets taken from benign tumors. An entrapment of membrane proteins could account for these results and should be considered when tissues are analyzed in the manner described.
Microfluorometric analysis of anti‐complement and indirect immunofluorescence tests for human papovavirus (JCV and BKV) T antigens
Authors: Beth E. , Cikes M. , Giraldo G. 
Published: February 2007

AbstractThe anti‐complement immunofluorescence (ACIF) test was compared with the conventionally used indirect IF (IIF) in regard to its usefulness for the detection of low amounts of human papovavirus tumor (T) antigen. Fluorescence microscopic analysis revealed that it is significantly more sensitive. Microfluorometric measurements on the intensity of staining of T‐antigen‐positive nuclei demonstrated that JCV T in HJC‐15 cells was 35‐fold and BKV T in BK‐L3 cells 94‐fold more intensively stained in the ACIF then in the IIF. It appears that the lower the actual amount of a given antigen the more valuable is the ACIF test.
Complement‐mediated lysis of type‐C virus: Effect of primate and human sera on various retroviruses
Authors: Sherwin Stephen A. , Benveniste Raoul E. , Todaro George J. 
Published: February 2007

AbstractAntibody‐independent complement‐mediated lysis of type‐C RNA tumor viruses (retroviruses) was measured in the sera of humans and other primates using the reverse transcriptase release assay. In this assay, preincubated mixtures of test serum and virus are assayed for the presence of viral reverse transcriptase released from disrupted virions. Serum decomplemented by heating possesses no such activity. Sera containing non‐specific heat‐labile factors inhibitory to the reverse transcriptase release assay are excluded from consideration. According to this assay, both normal and leukemic human sera, as well as sera from several other primate species including apes, Old World monkeys and New World monkeys, are all capable of lysing both endogenous and infectious primate and murine type‐C viruses. At least two non‐primate species (cat and rabbit) also possess this activity, although several other non‐primates do not. There is no appreciable viral specificity; each test serum possessing complement‐mediated virolytic activity can lyse all classes of retroviruses against which it is tested. Those primate species which spontaneously release retroviruses (baboon, squirrel monkey), as well as those primate species which are infected with exogenous retroviruses (rhesus, gibbon ape), are all capable of lysing their respective viruses. In addition, the relative efficiency of complement‐mediated viral lysis correlates better with primate phylogeny than with the relative ease of type‐C virus release or transmission. This would seem to suggest that complement‐mediated viral lysis does not, in fact, play a primary role in blocking the release or transmission of type‐C viruses among primates.
Type‐C oncovirus isolate from human leukemic bone marrow: Further in vitro and in vivo characterization
Authors: Nooter K. , Overdevest J. , Dubbes R. , Koch G. , Bentvelzen P. , Zurcher C. , Coolen J. , Calafat J. 
Published: February 2007

AbstractRabbit corneal cells transformed by a putative human type‐C helper virus pseudotype of the mouse sarcoma virus produce large amounts of transforming and non‐transforming viruses. The virions are antigenically related to the woolly monkey (simian) sarcoma‐leukemia type‐C oncovirus. Typical sarcoma virus lesions developed in newborn rats injected with virus‐producing rabbit cells. Cells producing only the putative type‐C helper viruses as a result of exposure to a high dilution of transforming virus stock induce lymphosarcomas after inoculation into newborn rats.
Increased immunogenicity of low‐antigenic rat tumors after superinfection with endogenous murine C‐type virus in nude mice
Authors: Kuzumaki Noboru , Fenyö Eva Maria , Giovanella Beppino C. , Klein George 
Published: February 2007

AbstractFour chemical carcinogen‐induced and two polyoma‐virus‐induced rat tumors were repeatedly passaged through nude mice. A methylcholanthrene‐induced tumor in BDIX rats (MBDB) and a polyoma‐virus‐induced tumor in Wistar/Fu rats (PW41) became infected with endogenous mouse virus (EMV), as judged by the expression of murine C‐type virus‐associated gp71, p30 and p12 antigens on their cell surface. Two ethylnitrosourea‐induced tumors in BDIX rats (290T and GE3A) were exposed in vitro to the supernatant of EMV‐infected PW41. Subsequently, 290T but not GE3A converted to murine gp71, p30 and p12 positivity. All these successfully infected rat tumors (EMV‐MBDB, EMV‐PW41 and EMV‐290T) became less transplantable to and more rejectable in otherwise susceptible syngeneic rats. To compare the immunogenicity of the virus‐infected and non‐infected tumors, syngeneic rats were immunized three times with irradiated cells, and challenged with the non‐infected tumor. Wistar/Fu rats immunized with irradiated EMV‐PW41 showed no improvement in PW41 rejection, compared to rate immunized with irradiated, non‐infected cells. On the other hand, BDIX rats immunized with EMV‐MBDB or EMV‐290T rejected MBDB or 290T, respectively, with no cross‐immunity, while the rats immunized with irradiated but non‐infected tumors showed no significant rejection. These results indicate that EMV infection augmented the immunogenicity of non‐immunogenic or only low‐immunogenic rat tumors.