bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acetylator phenotype in idiopathic systemic lupus erythematosus
Authors: Reidenberg Marcus M. , Levy Micha , Drayer Dennis E. , Zylber‐Katz Ester , Robbins William C. 
Published: February 2007

AbstractThe acetylator phenotypes of 21 patients from New York City and 29 patients from Israel with idiopthic systemic lupus erythematosus (SLE) were determined with dapsone or isoniazid. Thrity were slow acetylators, 17 were rapid, and 3 indeterminate. These cases plus published studies of acetylator phenotypes in indiopathic SLE show a worldwide distribution of 150 slow to 77 rapid instead of the expected 122:105 (P, < 0.001). The relationship between acetylator phenotype and possible etiologic agents is discussed.
Application of a radiometric ear assay for studies of adjuvant arthritis in rats
Authors: Gans K. R. , Heyner S. , Orzechowski R. F. 
Published: February 2007

AbstractA radioisotopic method, originally developed for measuring the cellular response in delayed hypersensitivity lesions in mice, has been evaluated in adjuvant arthritic rats. Focal accumulation of 5‐iodo‐2'‐deoxyuridine‐125I (125IUdR) at a site of antigen challenge (left pinna) was measured and expressed as increased radioactivity in the challenged (left) over the unchallenged (right) ear (L/R ear ratio). Immunologic specificity of the assay was established with anti‐lymphocyte globulin (ALG)‐treated arthritic rats. ALG significantly inhibited the 125IUdR L/R ear ratio; normal rabbit globulin had no effect on this parameter. A significant negative correlation was observed between the 125IUdR ear ratios and subjective arthritic scores in established adjuvant disease. Certain characteristics of the 125IUdR radiometric ear assay in rats were established in several types of experiments: disappearance of the isotope from blood, whole body irradiation studies in turpentine‐injected rats, and cyclophosphamide pretreatment in a sheep erythrocyte antigenic system. The results of this study support the utility of the 125IUdR ear assay to quantify cellular accumulation at a site of antigen challenge in adjuvant arthritic rats and possibly other antigenic systems in this species.
Presentation of occult giant cell arteritis
Authors: Healey Louis A. , Wilske Kenneth R. 
Published: February 2007

AbstractTemporal headache, blindness, and polymyalgia rheumatica are well‐recognized manifestions of giant cell arteritis. However, the disease may present in less evident fashion as shown by 30 of 74 patients with biopsy‐proven giant cell arteritis whose predominant complaint was not one of these cardinal symptoms. For this group of 30, the main problem was fever in 12 patients, anorexia, weight loss, and elevated serum alkaline phosphatase level suggesting an occult malignancy in 7, and unexplained anemia in 3. Four patients had a neurologic syndrome; 2 had diplopia and 2 acute weakness of one arm. Claudication was the chief complaint of 4 patients, involving the leg in 1, the arm in 1, and the jaw in 2. All patients responded well to steroid therapy.
Heterogeneity of the spontaneously expanded and mitogen‐induced generation of suppressor cell function of t cells on b cells in systemic lupus erythematosus
Authors: Ruiz‐Arguelles Alejandro , Alarcón‐Segovia Donato , Llorente Luis , Del Guidice‐Knipping JOSéa 
Published: February 2007

AbstractEighty percent of 31 untreated patients with systemic lupus erythematosus (SLE) and abnormalities in their spontaneously expanded and/or Con‐A‐induced suppressor cell function, but the association of defects detected with both systems was only 68%. Loss of spontaneous suppression related positively to disease activity (r = 0.641) and the number of Tγ cells (r = 0.624) whereas Con‐A‐induced suppression correlated negatively with disease activity (r = ‐0.456) and the number of Tγ cells (r = ‐0.089). Incubation of mononuclear cells from SLE patients in antiribonucleoprotein IgG caused further loss of suppression in some, but not all, instances. The suppressor cell dysfunction found in SLE may result from diverse mechanisms, including a basic defect in the generation of suppressor cells and the abrogation of suppressor function by autoantibodies.
Bone marrow as the major site of antierythrocyte autoantibody production in nzb mice
Authors: Cohen Philip L. 
Published: February 2007

AbstractBy use of a hemolytic plaque assay, it has been determined that most cells secreting the clinically important (anti‐X) erythrocyte autoantibody of NZB mice are located in the bone marrow. There was evidence of excessive polyclonal B cell activation in NZB spleen but not in bone marrow, despite the role of marrow as the major source of erythrocyte autoantibody. These findings suggest that polyclonal activation of B cells within the marrow does not lead to erythrocyte autoantibody production. Small but significant numbers of anti‐erythrocyte autoantibody producing cells were detected in the bone marrow but not in the spleens of normal mice, indicating that tolerance to erythrocytes may be less absolute in the bone marrow than in the spleen, or that the bone marrow serves as a repository for autoantibody secreting cells generated elsewhere.
The benign hypermobile joint syndrome
Authors: Jessee E. Forrest , Owen Duncan S. , Sagar Kiran B. 
Published: February 2007

AbstractBenign hypermobility as defined by a modification of the Carter‐Wilkinson criteria was found in 31 of 637 healthy blood donors, a prevalence of 5%. No increased prevalence of arthritis/arthralgia or characteristics suggestive of Ehlers‐Danlos syndrome other than a possible familial predisposition were found when these 31 hypermobile subjects were compared to an age and sex matched two‐for‐one control group. Cardiac evaluation by auscultation, electrocardiogram, and echocardiogram showed no increased occurrence of mitral valve prolapse in hypermobile subjects. Thus the benign hypermobile joint syndrome appears to be one extreme of the range of normal joint motion and not a systemic connective tissue disorder.
Bone cell cultures as an experimental model
Authors: Wong Glenda L. 
Published: February 2007

AbstractThe isolation and separation of bone cells with populations enriched for osteoclastic or osteoblastic phenotypes are described. Such systems offer the opportunity to compare and contrast the controls exerted by hormones, ions, and other agents on the functions of the individual bone cell types and may in the future provide explanations for the changes seen in bone tissue in various diseased states.
Association of hla‐drw4 with rheumatoid arthritis in black and white patients
Authors: Karr Robert W. , Rodey Glenn E. , Lee Tsung , Schwartz Benjamin D. 
Published: February 2007

AbstractThe HLA‐A, B, C, and DR antigens were typed in 35 black and 35 white Americans with rheumatoid arthritis. The frequency of HLA‐DRw4 was increased in both the black and white patient groups compared to the race‐matched control groups. DRw4 was found in 45.7% of the black patients compared to 14.3% of the black controls (corrected P value <0.035) and DRw4 was found in 71.4% of the white patients compared to 40.0% of the white controls (corrected P value <0.035). These data indicate that immunogenetic factors related to DRw4 are important in the development of rheumatoid arthritis in American blacks as well as whites.
Impaired antigen‐specific suppressor cell activity in patients with rheumatoid arthritis
Authors: Keystone Edward C. , Gladman Dafna D. , Buchanan Russel , Cane Donna , Poplonski Lorraine 
Published: February 2007

AbstractAntigen‐specific suppressor cell activity of peripheral blood mononuclear cells was investigated in 20 patients with rheumatoid arthritis (RA) and 16 age and sex‐matched healthy controls. Suppressor cell activity was generated by priming peripheral blood mononuclear cells with high dose antigen (ovalbumin) and adding the washed primed or control (unprimed) cells to autologous, optimally stimulated, target plaque forming cell (PFC) cultures. The ability of the primed cells to interfere with an optimal ovalbumin specific PFC response in the target culture was used as a measure of antigen‐specific suppressor cell activity. The results demonstrated that the mean (± SE) PFC response of the rheumatoid patients (669 ± 76 PFC/106 cells) was not statistically different from that of the normal controls (722 ± 83 PFC/106 cells), P = 0.1. However, reduced suppressor cell activity was observed in the rheumatoid patients relative to controls (46.4 ± 4.2% versus 64.6 ± 2.7% suppression, respectively; P < 0.001). No correlation was demonstrated between suppressor cell activity in rheumatoid patients and disease activity or therapy.
Permeability of hyaluronic acid solutions
Authors: Carr Marcus E. , Hadler Nortin M. 
Published: February 2007

AbstractThe permeability of hyaluronic acid solutions was measured in a chamber closed at each end by semipermeable membranes. Permeability was strongly concentration dependent below 1.0 gm% hyaluronic acid. Small changes in the ionic environment of 1.0 gm% hyaluronic acid solutions also altered permeability. The permeability of such solutions decreased by a factor of 4.7 in the presence of as little as 5 mM calcium ion and showed optimum permeability at pH 7.0. The pH optimum was not noted in hyaluronic acid preparations known to contain only small amounts of protein.
Treatment of behcet's syndrome with levamisole
Authors: De Merieux Paula , Spitler Lynn E , Paulus Harold E 
Published: February 2007

AbstractThe clinical response to levamisole in 11 patients with complete Behcet's syndrome was reviewed. Nine patients responded, 3 completely and 6 partially, with reduction in the number and severity of buccal and genital lesions. In 3 patients each, ocular inflammation and gastrointestinal involvement responded to levamisole, and in 1 patient neurologic status improved. Levamisole was purposely discontinued in 3 patients to assess its true role in disease control. Each patient experienced a flare. Reintroduction of therapy controlled the flare in all cases, although 1 patient subsequently relapsed while continuing therapy. Two patients failed to respond. Side effects necessitated permanent discontinuation of the drug in 2 respondent patients, but in no case did neutropenia or agranulocytosis develop. These preliminary results suggest that levamisole may be useful in the therapy of the various manifestations of Behcet's syndrome and that a controlled prospective study is indicated.
Polyclonally triggered B cells in the peripheral blood and bone marrow of normal individuals and in patients with systemic lupus erythematosus and primary sjögren's syndrome
Authors: Fauci Anthony S. , Moutsopoulos Haralampos M. 
Published: February 2007

AbstractNumbers of B cells spontaneously secreting Ig (IgG, IgA, and IgM) were determined by a plaque‐forming cell (PFC) assay simultaneously in the peripheral blood and bone marrow of normal individuals, patients with systemic lupus erythematosus (SLE), and patients with primary Sjögren's syndrome. Normal individuals had 382 (± 89) PFC per 106 mononuclear cells in peripheral blood. Patients with either active or inactive Sjögren's syndrome had normal numbers of spontaneous Ig‐secreting cells in peripheral blood (P > 0.2). Conversely, patients with inactive as well as active SLE had markedly increased spontaneous PFC (P < 0.05 and P < 0.001, respectively). Patients with active SLE had significantly greater PFC than patients with inactive SLE: 3,984 (± 960) versus 1,605 (± 527) PFC per 106 mononuclear cells (P < 0.05). The lack of increased numbers of activated B cells in the blood of patients with Sjögren's syndrome was not explained by a preferential sequestration of activated B cells in the bone marrow. However, of particular interest was the finding that the bone marrow served not only as a major source of virgin B cells but as a lymphoid organ of either in situ activation of B cells or sequestration for activated B cells. Normal individuals had approximately a 20‐fold relative increase of activated B cells per 106 mononuclear cells in the bone marrow compared to peripheral blood, while patients with inactive and active SLE both had a 35‐fold relative increase in activated B cells in bone marrow compared to peripheral blood. The potential relevance of circulating activated B cells and their sequestration in lymphoid organs is discussed concerning the discrepancy in this regard between Sjögren's syndrome and SLE, and our understanding of the significance of polyclonal B cell activation in the pathogenesis of these diseases.
Effect of high‐dose methylprednisolone infusion on polymorphonuclear leukocyte function in patients with systemic lupus erythematosus
Authors: Daniel Perez H. , Kimberley Robert P. , Kaplan Howard B. , Edelson Henry , Inman Robert D. , Goldstein Ira M. , Mellon Andrew W. 
Published: February 2007

AbstractWe have studied the effect of high‐dose (1 gm) methylprednisolone infusion on polymorphonuclear leukocyte (PMN) function in 11 patients with active systemic lupus erythematosus (SLE). The only alteration of polymorphonuclear leukocyte function produced consistently by methylprednisolone was decreased adherence to plastic surfaces when tested 2 hours after infusion. This steroid‐induced abnormality, however, was transient. Cells obtained from patients 24 hours after a single dose of drug exhibited normal adhesiveness. These results indicate that single, large doses of methylprednisolone do not produce long‐lasting abnormalities of PMN function in patients with lupus.
Three siblings with scleroderma (systemic sclerosis) and two with raynaud's phenomenon from a single kindred
Authors: Sheldon William B. , Lurie David P. , Maricq Hildegard R. , Bashar Kahaleh M. , Delustro Frank A. , Gibofsky Allan , Carwile Leroy E. 
Published: February 2007

AbstractA kindred is reported which contains 3 siblings with scleroderma, 2 siblings with Raynaud's phenomenon, and 2 first‐degree relatives with histories suggestive of connective tissue syndromes. Studies of microvascular morphology and flow, serum endothelial cytotoxic activity, antinuclear antibodies, and HLA haplotypes in 18 relatives and 6 spouses revealed that 4 of 5 affected siblings expressed the HLA‐DRw4 antigen, which was also present in 2 of 3 asymptomatic relatives whose serum contained endothelial cytotoxic activity. The evidence for an inherited susceptibility to scleroderma is reviewed.
Proteoglycans from normal and degenerate cartilage of the adult human tibial plateau
Authors: Santer Vivien , White Robert J. , Roughley Peter J. 
Published: February 2007

AbstractProteoglycans were extracted from normal and degenerate cartilage of the human tibial plateau. Both areas possessed proteoglycans of similar chemical composition, though the degenerate cartilage contained a greater proportion of molecules of lower buoyant density and enriched in keratan sulfate. There was no evidence for the changes in glycosaminoglycan synthesis that have been described for clinically osteoarthritic cartilage, or for changes in the ability to aggregate with hyaluronic acid.
Effect of cyclophosphamide, hydrocortisone, and levamisole on collagen‐induced arthritis in rats
Authors: Stuart John M. , Myers Linda K. , Townes Alexander S. , Kang Andrew H. 
Published: February 2007

AbstractThe immune response of rats to type II collagen was modified by pretreating the rats with levamisole, hydrocortisone, or cyclophosphamide. Rats were observed for the development of collagen‐induced arthritis and were bled serially for the determination of antibody levels to type II collagen. Levamisole had a slight but not significant potentiating effect with arthritis developing earlier, whereas hydrocortisone and cyclophosphamide significantly depressed the incidence of arthritis; cyclophosphamide also delayed its onset. Antibody levels measured by radioimmunoassay paralleled the clinical course of arthritis.
Blind evaluation of the diagnostic specificity of nailfold capillary microscopy in the connective tissue diseases
Authors: Kenik Jay G. , Maricq Hildegard R. , Bole Giles G. 
Published: February 2007

AbstractTwenty‐four patients with connective tissue diseases and 5 control subjects were studied by “wide‐field” photography. A total of 44 photographs of the nailfold were sent to the second author (HRM) for “blind” diagnostic interpretation. The scleroderma‐dermatomyositis pattern was identified in 17 of 18 photographs of 11 scleroderma patients, and 5 of 5 photographs of 2 dermatomyositis patients. Six of 8 photographs of 5 patients with systemic lupus erythematosus were correctly identified, as were 7 of 7 photographs of 5 control subjects. These results demonstrate that characteristic patterns of nailfold capillary abnormalities can be identified and correlated blindly with the clinical diagnosis in several connective tissue diseases.
Hla antigens in essential mixed cryoglobulinemia
Authors: Migliorini Paola , Bombardieri Stefano , Castellani Alberto , Ferrara Giovan Battista 
Published: February 2007

AbstractHLA antigens were studied in 36 patients with essential mixed cryoglobulinemia. The antigens that showed the largest percentage of deviations from the control group were DRw3 (40% versus 20%), DRw8 (32% versus 11.5%), and Cw6 (5.6% versus 20.7%). None of these changes, however, was significant when the P values were corrected for the number of specificities scored for each locus. These data indicate that essential mixed cryoglobulinemia is not strongly associated with any of the HLA antigens serologically determined, but there was a suggestion that certain manifestations, particularly renal involvement, are related to a specific haplotype.
The role of histones as nuclear autoantigens in drug‐related lupus erythematosus
Authors: Tan Eng M. , Portanova Joseph P. 
Published: February 2007

AbstractPatients with drug‐related lupus erythematosus produce antibodies to nuclear histones which can be detected by a three‐step indirect immunofluorescent technique. Procainamide‐related antinuclear antibodies were detected by this technique, but hydralazine‐related antinuclear antibodies were not. Certain evidence suggests that antibodies induced by the two drugs are reactive with different subclasses of histones. Hydralazine was shown to interact with a soluble DNA‐histone complex, and the resulting interaction rendered the histone moiety resistant to trypsin digestion. This mechanism may help to maintain DNA‐histone complexes in a potentially immunogenic form and result in the production of autoantibodies.
Urinary excretion of sialic acid‐containing saccharides in systemic lupus erythematosus
Authors: Maury C. Peter J. , Sjöblom Clara , Wegelius Otto 
Published: February 2007

AbstractUrinary sialic acid‐containing trisaccharides, total sialic acid, and serum sialic acid were studied in 17 patients with systemic lupus erythematosus (SLE) and in 15 healthy controls. The urinary excretion of sialyllactose, measured by a gas chromatographic method, was significantly greater in patients with SLE (37.4 ± 21.4 mg/24 hours, SD) than in the control subjects (13.7 ± 3.8 mg/24 hours, P < 0.001). The mean excretion of sialyl‐N‐acctyllactosamine (16.6 ± 8.5 mg/24 hours) and total sialic acid (82.5 ± 29.4 mg/24 hours) was also greater in the SLE group than in the controls (8.7 ± 2.8 and 58.0 ± 16.0 mg/24 hours, respectively; P < 0.01). Serum levels of sialic acid were correspondingly higher in the SLE patients (84.4 ± 20.4 mg/100 ml) than in the controls (63.7 ± 6.5 mg/100 ml, P < 0.001). Urinary excretion of sialyl‐lactose correlated positively with clinical disease activity (P < 0.001) and with anti‐DNA antibody levels (P < 0.05). On the average, patients with moderate or severe disease excreted three times more sialyl‐lactose than did those with mild or inactive disease. Our results suggest that the excretion of sialyl‐oligosaccharides reflects disease activity in SLE.
Acetylator phenotype and metabolic disposition of isoniazid in japanese patients with systemic lupus erythematosus
Authors: Ishizaki Takashi , Horai Yukio , Koya Gyoichi , Matsuyama Kenji , Iguchi Sadao 
Published: February 2007

AbstractAcetylator phenotype and metabolic disposition of isoniazid (INH) were studied in 19 Japanese (a population shown to be 11.5% slow acetylators) patients with spontaneous systemic lupus erythematosus (SLE) and 19 healthy controls. Subjects with the elimination half‐life (t1/2) of INH of 2.0 hours or less were considered rapid and those of 2.2 hours or more were slow acetylators. Results of phenotyping showed that 17 of 19 SLE patients were rapid, 1 slow, and 1 indeterminate, whereas 18 of the controls were rapid and 1 indeterminate. When phenotyped according to another reported antimode (107 or 110 minutes), 3 of the patients and 2 of the controls were slow and the remainder were all rapid acetylators. The distribution of INH t1/2, acetyl INH to INH ratios in urine and plasma, and hydrazine compounds in urine measured with gas chromatographymass spectrometry was similar between the two groups, except for 1 patient who was definitely classified as a slow acetylator. The relationship between phenotype distribution and possible pathoetiologic factors is discussed.
Cytotoxicity of anti‐fab antibodies against b lymphocytes in rheumatoid arthritis
Authors: Nasu Hajime , Chia David , Iwaki Yuichi , Barnett Eugene V. , Terasaki Paul I. 
Published: February 2007

AbstractAnti‐Fab antibody titers were positive in 70% of rheumatoid arthritis patients (54 of 77) and 3% (1 of 35) of healthy donors. Their specificity was examined by inhibition of the radioimmunoassay for anti‐Fab antibodies, which demonstrated that they are against the Fd region of the intact immunoglobulin molecule. In addition, anti‐Fab antibodies have broad specificities for IgG antigens, shown by inhibition with myeloma IgG. They are cytotoxic against B (20% of B cells from most healthy donors) but not T lymphocytes, with cytotoxicity greater at 5°C than 37°C. These studies show that the anti‐Fab antibodies interact with allotypic or idiotypic determinants on subpopulations of B lymphocyte cell surface antigens.
The Influence of Fibronectin on Cryoprecipitate Formation in Rheumatoid Arthritis and Systemic Lupus Erythematosus
Authors: Beaulieu André D. , Valet Jean P. , Strevey Johanne 
Published: February 2007

AbstractFibronectin, a human fibroblast surface and plasma protein, is present in cryoprecipitates of synovial fluids and/or sera from patients with rheumatoid arthritis, systemic lupus erythematosus, ankylosing spondylitis, and mixed essential cryoglobulinemia. Fibronectin was shown to be capable of influencing cryoprecipitate formation. No antibodies to fibronectin could be detected, thus ruling out the possibility that it was directly involved in the formation of cold‐insoluble antigen—antibody complexes. Fibrinogen or fibrinogen degradation products were frequently present in synovial fluid cryoprecipitates but rarely in serum cryoprecipitates. Since complexes of fibronectin—fibrin—fibrinogen are known to be cold‐insoluble, such interactions could occur in synovial fluids and contribute to the formation of cryoprecipitates. In serum, however, this is not likely to occur, and the mechanism by which fibronectin influences cryoprecipitate formation remains to be elucidated.