bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a major QTL and candidate genes analysis for branch angle in rapeseed (Brassica napus L.) using QTL-seq and RNA-seq
Authors: Lei Shaolin , Chen Li , Liang Fenghao , Zhang Yuling , Zhang Chao , Xiao Huagui , Tang Rong , Yang Bin , Wang Lulu , Jiang Huanhuan 
Published: February 2024

IntroductionBranching angle is an essential trait in determining the planting density of rapeseed (Brassica napus L.) and hence the yield per unit area. However, the mechanism of branching angle formation in rapeseed is not well understood.MethodsIn this study, two rapeseed germplasm with extreme branching angles were used to construct an F2 segregating population; then bulked segregant analysis sequencing (BSA-seq) and quantitative trait loci (QTL) mapping were utilized to localize branching anglerelated loci and combined with transcriptome sequencing (RNA-seq) and quantitative real-time PCR (qPCR) for candidate gene miningResults and discussionA branching angle-associated quantitative trait loci (QTL) was mapped on chromosome C3 (C3: 1.54-2.65 Mb) by combining BSA-seq as well as traditional QTL mapping. A total of 54 genes had SNP/Indel variants within the QTL interval were identified. Further, RNA-seq of the two parents revealed that 12 of the 54 genes were differentially expressed between the two parents. Finally, we further validated the differentially expressed genes using qPCR and found that six of them presented consistent differential expression in all small branching angle samples and large branching angles, and thus were considered as candidate genes related to branching angles in rapeseed. Our results introduce new candidate genes for the regulation of branching angle formation in rapeseed, and provide an important reference for the subsequent exploration of its formation mechanism.
Are depressive symptoms linked to a reduced pupillary response to novel positive information?—An eye tracking proof-of-concept study
Authors: Spaeth Alexandra M. , Koenig Stephan , Everaert Jonas , Glombiewski Julia A. , Kube Tobias 
Published: February 2024

IntroductionDepressive symptoms have been linked to difficulties in revising established negative beliefs in response to novel positive information. Recent predictive processing accounts have suggested that this bias in belief updating may be related to a blunted processing of positive prediction errors at the neural level. In this proof-of-concept study, pupil dilation in response to unexpected positive emotional information was examined as a psychophysiological marker of an attenuated processing of positive prediction errors associated with depressive symptoms.MethodsParticipants (N = 34) completed a modified version of the emotional Bias Against Disconfirmatory Evidence (BADE) task in which scenarios initially suggest negative interpretations that are later either confirmed or disconfirmed by additional information. Pupil dilation in response to the confirmatory and disconfirmatory information was recorded.ResultsBehavioral results showed that depressive symptoms were related to difficulties in revising negative interpretations despite disconfirmatory positive information. The eye tracking results pointed to a reduced pupil response to unexpected positive information among people with elevated depressive symptoms.DiscussionAltogether, the present study demonstrates that the adapted emotional BADE task can be appropriate for examining psychophysiological aspects such as changes in pupil size along with behavioral responses. Furthermore, the results suggest that depression may be characterized by deviations in both behavioral (i.e., reduced updating of negative beliefs) and psychophysiological (i.e., decreased pupil dilation) responses to unexpected positive information. Future work should focus on a larger sample including clinically depressed patients to further explore these findings.
Collectivism, face concern and Chinese-style lurking among university students: the moderating role of trait mindfulness
Authors: Hu Bing , Zhu Yi , Liu Chao , Zheng Shanshan , Zhao Ziying , Bao Ruxiang 
Published: February 2024

IntroductionThis study focuses on understanding the unique causes and mechanisms of “Chinese-style lurking” on WeChat among university students, within a cultural context that emphasizes collectivism and face concern. The research also looks into the moderating role of trait mindfulness.MethodsFor the confirmation of these phenomena and to validate the theories, a structural equation model was constructed using the Stress-Strain-Outcome (SSO) theory and mindfulness buffering theory. The model was then tested and validated with data from 1,453 valid online surveys. These data were analyzed using the SmartPLS 4.0 software.ResultsThe results indicate that collectivism increases face concern, which in turn escalates online social anxiety. Face concern completely mediates between collectivism and online social anxiety, creating a serial mediation effect between face concern, online social anxiety, and lurking behavior. Additionally, trait mindfulness was found to negatively modulate the pathways from collectivism to face concern and from online social anxiety to lurking.DiscussionThe findings underscore the influence of traditional Chinese culture on contemporary students' online behavior and provide a new perspective for understanding social media lurking in an Eastern context. The results suggest that a mindfulness-based approach could be used to mitigate the associated silence and anxiety.
Persistent neurocognitive deficits in cognitively impaired survivors of sepsis are explained by reductions in working memory capacity
Authors: Kattlun Fabian , Hertel Elizabeth , Geis Christian , Scherag André , Wickel Jonathan , Finke Kathrin 
Published: February 2024

IntroductionSepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Mounting evidence suggests that many cognitively impaired sepsis survivors show long-term neurocognitive deficits in neuropsychological tasks. To date, the underlying mechanisms of these deficits are insufficiently understood. Based on previous evaluations we hypothesized that visual attention and working memory may be affected in a sample of cognitively impaired sepsis survivors.MethodsWe utilized psychophysical whole-and partial-report paradigms based on the computational theory of visual attention (TVA) to determine (i) whether sepsis survivors show changes in basic parameters of visual attention and working memory, (ii) whether the affected parameters are related to neuropsychological test results in a standard battery in sepsis survivors and matched healthy control participants, (iii) whether between-group differences in these basic parameters of visual attention could account for underperformance of sepsis survivors in neuropsychological tests when adjusting for potentially relevant clinical variables.ResultsWe showed that, in sepsis survivors, the maximum number of elements consciously maintained in an instant, i.e. the working memory storage capacity K, is reduced (sepsis survivors: M = 3.0; healthy controls: M = 3.4). Moreover, K explained variance in neurocognitive outcomes –17% in attentional and 16 % in executive functions – in a standard neuropsychological battery. The association remained stable when adjusting for clinical variables.DiscussionThus, in our sample of cognitively impaired sepsis survivors, a reduction in working memory capacity seems to be a critical determinant of the neurocognitive sequelae. It should be the subject of future work on mechanisms but may also serve as surrogate outcome measure in interventional studies.
Emotional self-states and coping responses in patients with chronic tinnitus: a schema mode model approach
Authors: Boecking Benjamin , Stoettner Eva , Brueggemann Petra , Mazurek Birgit 
Published: February 2024

BackgroundAmongst “third-wave” cognitive behavioural therapies, schema therapy demonstrates encouraging efficacy across various mental health conditions. Within this field, clinical interest has begun to converge on the “schema-mode-model” – a conceptualization framework for affective, cognitive and behavioral states that guide individuals’ perceptions and behaviours at a given point in time. Schema mode expressions in patients with chronic tinnitus are as-yet unexamined.MethodsThe present study reports self-report data from N = 696 patients with chronic tinnitus who completed the Schema Mode- and Tinnitus Handicap Inventories alongside measures of perceived stress, anxiety and depression. The Schema Mode Inventory assesses so-called maladaptive “parent”, “child” and “coping” modes. Parent modes can be understood as self-states which are characterized by self-critical and hostile beliefs; child modes are characterized by biographically unmet emotional needs; and coping modes by inflexible attempts to regulate emotion and stabilize one’s sense of self. Descriptive, correlational and mediation analyses investigated schema mode expressions (1) in patients with chronic tinnitus, (2) as compared to published reference values from a healthy control sample, (3) in their relation to other psychological constructs, and (4) regarding their potential role in driving tinnitus-related distress.ResultsPatients reported mild-to-moderate levels of emotional distress. Compared to healthy controls, patients showed (1) high relative expressions of child-, detachment and compliant coping modes and (2) a conspicuously low relative expression of the ‘punitive parent’ mode. Correlational patterns suggested strong associations of (1) parent as well as angry child modes with perceived stress and anxiety, (2) the vulnerable child mode with all measured constructs and (3) emotional distress with - intrapersonally - emotional detachment as well as - interpersonally - alleged compliance. Mediation analyses demonstrated that tinnitus-related distress was driven by significant interactions between child and coping modes.ConclusionsThe study provides initial clinical evidence for the relevance and applicability of schema-mode based formulation and treatment planning in patients with chronic tinnitus.
Effectiveness of decentralizing outpatient acute malnutrition treatment with community health workers and a simplified combined protocol: a cluster randomized controlled trial in emergency settings of Mali
Authors: López-Ejeda Noemí , Charle-Cuéllar Pilar , Samake Salimata , Dougnon Abdias Ogobara , Sánchez-Martínez Luis Javier , Samake Mahamadou N’tji , Bagayoko Aliou , Bunkembo Magloire , Touré Fanta , Vargas Antonio , Guerrero Saul 
Published: February 2024

BackgroundOutpatient treatment of acute malnutrition is usually centralized in health centers and separated into different programs according to case severity. This complicates case detection, care delivery, and supply chain management, making it difficult for families to access treatment. This study assessed the impact of treating severe and moderate cases in the same program using a simplified protocol and decentralizing treatment outside health centers through community health workers (CHWs).MethodsA three-armed cluster randomized controlled trial under a non-inferiority hypothesis was conducted in the Gao region of Mali involving 2,038 children between 6 and 59 months of age with non-complicated acute malnutrition. The control arm consisted of 549 children receiving standard treatment in health centers from nursing staff. The first intervention arm consisted of 800 children treated using the standard protocol with CHWs added as treatment providers. The second intervention arm consisted of 689 children treated by nurses and CHWs under the ComPAS simplified protocol, considering mid-upper arm circumference as the sole anthropometric criterion for admission and discharge and providing a fixed dose of therapeutic food for severe and moderate cases. Coverage was assessed through cross-sectional surveys using the sampling evaluation of access and coverage (SLEAC) methodology for a wide area involving several service delivery units.ResultsThe recovery rates were 76.3% in the control group, 81.8% in the group that included CHWs with the standard protocol, and 92.9% in the group that applied the simplified protocol, confirming non-inferiority and revealing a significant risk difference among the groups. No significant differences were found in the time to recovery (6 weeks) or in anthropometric gain, whereas the therapeutic food expenditure was significantly lower with the simplified combined program in severe cases (43 sachets fewer than the control). In moderate cases, an average of 35 sachets of therapeutic food were used. With the simplified protocol, the CHWs had 6% discharge errors compared with 19% with the standard protocol. The treatment coverage increased significantly with the simplified combined program (SAM +42.5%, MAM +13.8%).ImplicationsImplementing a simplified combined treatment program and adding CHWs as treatment providers can improve coverage while maintaining non-inferior effectiveness, reducing the expenditure on nutritional intrants, and ensuring the continuum of care for the most vulnerable children.
Factors related to satisfaction with community-based home aging services in Shandong, China
Authors: Wang Yujing , Zhang Qi , Huang Laigang , Zeng Fanshuo 
Published: February 2024

PurposeThis study investigated the satisfaction of current community-based home care services and its factors in adults aged ≥60 years.MethodsUsing stratified cluster random sampling, we surveyed 1,494 older adults in Jinan and Qingdao, Shandong province, between 2021 and 2023. The baseline and satisfaction surveys were designed by our research team, and the questionnaires were conducted in the form of structured interviews. Kruskal-Wallis H-test and Logistic regression analysis were used to explore the influencing factors of satisfaction.ResultsThe satisfaction was mainly affected by age (p = 0.007), marital status (p &lt; 0.001), pre-retirement occupation (p = 0.003), economic source (p &lt; 0.001), and mode of residence (p = 0.001) in the study of 1,494 older adults. Under the influence of multiple factors, the evaluation of older adults services, married [OR = 4.039, 95% CI: 1.176–13.877] were more inclined to be average, and their occupations were agriculture, forestry, animal husbandry, fishery, and water production workers [OR = 0. 237, 95% CI: 0.068–0.819] and production and transportation equipment operators and related personnel [OR = 0.153, 95% CI: 0.024–0.966] or [OR = 0.153, 95% CI: 0.029–0.820] tended to be more dissatisfied.ConclusionThe satisfaction level of community-based home care services is relatively high among older adults, and it is mainly affected by factors such as age, marital status, pre-retirement occupation, source of financial resources, and mode of residence. Addressing the emotional needs of older adults, lowering the cost of aging, and integrating health care and aging seamlessly are among the ongoing challenges that we need to tackle.
Trees and sidewalks: toward an infrastructure protection approach
Authors: Otero-Durán Laura , Torres Andrés 
Published: February 2024

IntroductionNature-based solutions are increasingly recognized as vital components of urban resilience strategies, particularly within the framework of green infrastructure. This study aims to propose an approach that fosters symbiosis between green and gray infrastructure to address the challenges posed by climate change in urban environments.MethodsWe conducted a comprehensive review of guidelines and scientific literature to inform the selection of species and the design of root containers for urban tree planting. Additionally, we performed a multicriteria analysis and assessed water comfort to guide decision-making regarding species selection in specific city areas.ResultsThe methodology was applied to a case study in Bogotá, yielding insights applicable to any city with basic knowledge of suitable species for planting in built public spaces. Crucial criteria for selecting local species for sidewalks were identified, including size, permeability, soil compaction characteristics, and climatic adaptability. A list of desirable species adapted to all humidity zones of the case study city was generated. Hydrological sizing methods proposed are contingent upon both the species to be planted and the geometry of the streets.DiscussionThe approach and findings presented in this study promote the development of trees and their ecosystem services while mitigating potential damage to surrounding infrastructure.ConclusionImplementing strategies that facilitate symbiosis between green and gray infrastructure contributes to urban resilience and aids in climate change adaptation efforts.
Effect of the pre-taper level of fatigue on the taper-induced changes in performance in elite swimmers
Authors: Bretonneau Quentin , Morales-Artacho Antonio , Pla Robin , Bosquet Laurent 
Published: February 2024

IntroductionIn swimming, performance gains after tapering could be influenced by the pre-taper level of fatigue. Moreover, this level of fatigue could be associated with sleep. This study aimed to assess (1) the effect of tapering on performance according to the pre-taper level of fatigue in swimmers and (2) the association between sleep and pre-taper level of fatigue.MethodsPhysiological, psychological and biomechanical profiles were evaluated in 26 elite swimmers on 2 occasions to estimate the pre-taper level of fatigue: at T0 and T1, scheduled respectively 10 and 3 weeks before the main competition. Sleep quantity and quality were also evaluated at T0 and T1. Race time was officially assessed at T0, T1 and during the main competition. The level of significance was set at p ≤ .05.ResultsFourteen swimmers (17 ± 2 years) were allocated to acute fatigue group (AF) and 12 swimmers (18 ± 2 years) to functional overreaching group (F-OR). From T1 to the main competition, performance was improved in AF (+1.80 ± 1.36%), while it was impaired in F-OR (−0.49 ± 1.58%, p &lt; 0.05 vs. AF). Before taper period, total sleep time was lower in F-OR, as compared to AF. Conversely, the fragmentation index was higher in F-OR (p = .06). From wakefulness to sleep, body core temperature decreased in AF but not in F-OR.DiscussionPerformance gain after tapering was higher in AF swimmers than in overreached. Moreover, pre-taper sleep was poorer in overreached swimmers, which could contribute to their different response to the same training load. This poorer sleep could be linked to a lower regulation of internal temperature.
Exploring countermovement jump variables across competitive levels and playing positions in futsal
Authors: Spyrou Konstantinos , Alcaraz Pedro E. , Martínez-Serrano Antonio , Marín-Cascales Elena , Ferioli Davide , Contreras Jean Paul Santis , Ribeiro João Nuno , Travassos Bruno , Freitas Tomás T. 
Published: February 2024

IntroductionThe aims of this study were to compare several countermovement jump (CMJ) kinetic variables between professional (PRO) and semi-professional (SEMI-PRO) futsal players and examine the differences amongst playing positions.MethodsCMJ performance from 56 male futsal players (25.2 ± 4.8 years; weight: 74.4 ± 6.4 kg) was analysed. Players were separated into PRO (n = 29; 27.0 ± 4.4 years; 75.4 ± 6.0 kg) and SEMI-PRO (n = 27; 22.7 ± 4.3 years; 73.1 ± 6.8 kg), and according to playing position: defenders (n = 16; 25.4 ± 3.7 years; 75.2 ± 6.0 kg), wingers (n = 26; 23.5 ± 4.5 years; 72.0 ± 6.9 kg), and pivots (n = 14; 28.0 ± 5.6 years; 77.8 ± 4.3 kg). Linear mixed models and effect sizes were used for the analyses based on the mean of two jumps for each variable.ResultsPRO players presented a deeper center of mass (COM) displacement (p = 0.002, ES = 0.83), greater eccentric (Ecc) absolute (p = 0.019, ES = 0.61) and relative peak power (p = 0.046, ES = 0.52), and achieved greater Ecc peak velocities (p = 0.004, ES = 0.76) when compared to SEMI-PRO. Non-significant and trivial-to-small differences were observed in all the other CMJ variables according to the competitive level and playing position.DiscussionEcc capabilities (i.e., deeper COM displacement, greater Ecc absolute and relative peak power, and peak velocity) during vertical jump seem to differentiate PRO and SEMI-PRO players. However, CMJ variables do not discriminate amongst playing positions in futsal players.
An In Silico and In Vitro Assessment of the Neurotoxicity of Mefloquine
Authors: El Sharazly Basma M. , Ahmed Abrar , Elsheikha Hany M. , Carter Wayne G. 
Published: February 2024

Mefloquine (MQ) is a quinoline-based anti-malarial drug used for chemoprophylaxis or as a treatment in combination with artesunate. Although MQ has clear anti-Plasmodium falciparum properties, it can induce neurotoxicity and undesired neuropsychiatric side effects in humans. Hence, this study aimed to characterize the neurotoxicity of MQ using human neuroblastoma SH-SY5Y cells. The effects of MQ on neuronal toxicity and cell viability were investigated over a concentration range of 1–100 µM using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The influence of MQ on cellular bioenergetics was examined by measuring cellular ATP levels and from the induction of reactive oxygen species (ROS). An in silico approach was used to assess the potential neurotoxicity of MQ mediated via binding to the active sites of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) and then experimentally validated via in vitro enzymatic assays. MQ was cytotoxic to neuronal cells in a concentration and exposure duration dependent manner and induced a significant reduction in viability at concentrations of ≥25 µM after a 24 h exposure. MQ adversely impacted cellular bioenergetics and significantly depleted ATP production at concentrations of ≥1 µM after 24 h. MQ-induced cellular ROS production, which was correlated with the induction of apoptosis, as revealed by flow cytometry. In silico studies suggested that MQ was a dual cholinesterase inhibitor and one with remarkably potent binding to BuChE. Modelling data were supported by in vitro studies which showed that MQ inhibited both human AChE and BuChE enzymes. In summary, MQ is an antimalarial drug that may induce neurotoxicity by impacting cellular bioenergetics and perturbing the activity of cholinesterases at exposure concentrations relevant to human dosage.
Three New Periconia Species Isolated from Wurfbainia villosa in Guangdong, China: A Discussion on the Doubtful Taxa Clustering in this Genus
Authors: Liao Chunfang , Hyde Kevin D. , Thilini Chethana Kandawatte Wedaralalage , Dong Wei , Yang Yunhui , Doilom Mingkwan 
Published: February 2024

During a survey of fungi on Wurfbainia villosa in Guangdong Province, China, three novel species, Periconia endophytica, P. yangjiangensis, and P. wurfbainiae, belonging to Periconiaceae in Pleosporales, Dothideomycetes are proposed based on morphological and phylogenetic evidence. Periconia endophytica was isolated from the healthy leaves of W. villosa, while P. yangjiangensis and P. wurfbainiae were obtained from the dead stems of the same host. Notably, holomorphs were observed in P. wurfbainiae. The morphological characteristics of the novel taxa are compared with closely related species within Periconia. Illustrations, morphological descriptions, and phylogenetic analyses are provided for the novel taxa. Multilocus phylogeny of the combined internal transcribed spacer (ITS), large subunit nuclear rDNA (LSU), small subunit nuclear ribosomal rDNA (SSU), and partial translation elongation factor 1–α (tef1-α) regions supported the establishment of three new species. Furthermore, the taxa clustering in Periconia, Flavomyces fulophazii, and Sporidesmium tengii, are discussed for further investigation of their taxonomic placements.