bluuebunny/crossref_metadata_2025_split huggingface dataset:
An interaction graph approach to gain new insights into mechanisms that modulate cerebrovascular tone
Authors: Dempsey Sergio , Argus Finbar , Maso Talou Gonzalo Daniel , Safaei Soroush 
Published: April 2024

AbstractMechanisms to modulate cerebrovascular tone are numerous, interconnected, and spatially dependent, increasing the complexity of experimental study design, interpretation of action-effect pathways, and mechanistic modelling. This difficulty is exacerbated when there is an incomplete understanding of these pathways. We propose interaction graphs to break down this complexity, while still maintaining a holistic view of mechanisms to modulate cerebrovascular tone. These graphs highlight the competing processes of neurovascular coupling, cerebral autoregulation, and cerebral reactivity. Subsequent analysis of these interaction graphs provides new insights and suggest potential directions for research on neurovascular coupling, modelling, and dementia.
Rhythmicity of neuronal oscillations delineates their cortical and spectral architecture
Authors: Myrov Vladislav , Siebenhühner Felix , Juvonen Joonas J. , Arnulfo Gabriele , Palva Satu , Palva J. Matias 
Published: April 2024

AbstractNeuronal oscillations are commonly analyzed with power spectral methods that quantify signal amplitude, but not rhythmicity or ‘oscillatoriness’ per se. Here we introduce a new approach, the phase-autocorrelation function (pACF), for the direct quantification of rhythmicity. We applied pACF to human intracerebral stereoelectroencephalography (SEEG) and magnetoencephalography (MEG) data and uncovered a spectrally and anatomically fine-grained cortical architecture in the rhythmicity of single- and multi-frequency neuronal oscillations. Evidencing the functional significance of rhythmicity, we found it to be a prerequisite for long-range synchronization in resting-state networks and to be dynamically modulated during event-related processing. We also extended the pACF approach to measure ’burstiness’ of oscillatory processes and characterized regions with stable and bursty oscillations. These findings show that rhythmicity is double-dissociable from amplitude and constitutes a functionally relevant and dynamic characteristic of neuronal oscillations.
MIM-CyCIF: masked imaging modeling for enhancing cyclic immunofluorescence (CyCIF) with panel reduction and imputation
Authors: Sims Zachary , Mills Gordon B. , Chang Young Hwan 
Published: April 2024

AbstractCyclic Immunofluorescence (CyCIF) can quantify multiple biomarkers, but panel capacity is limited by technical challenges. We propose a computational panel reduction approach that can impute the information content from 25 markers using only 9 markers, learning co-expression and morphological patterns while concurrently increasing speed and panel content and decreasing cost. We demonstrate strong correlations in predictions and generalizability across breast and colorectal cancer, illustrating applicability of our approach to diverse tissue types.
Effects of multi-functional additives during foam extrusion of wheat gluten materials
Authors: Bettelli Mercedes A. , Hu Qisong , Capezza Antonio J. , Johansson Eva , Olsson Richard T. , Hedenqvist Mikael S. 
Published: April 2024

Abstract To broaden the range in structures and properties, and therefore the applicability of sustainable foams based on wheat gluten expanded with ammonium-bicarbonate, we show here how three naturally ocurring multifunctional additives affect their properties. Citric acid yields foams with the lowest density (porosity of ~50%) with mainly closed cells. Gallic acid acts as a radical scavenger, yielding the least crosslinked/ aggregated foam. The use of a low amount of this acid yields foams with the highest uptake of the body-fluid model substance (saline, ~130% after 24 hours). However, foams with genipin show a large and rapid capillary uptake (50% in one second), due to their high content of open cells. The most dense and stiff foam is obtained with one weight percent genipin, which is also the most crosslinked. Overall, the foams show a high energy loss-rate under cyclic compression (84-92% at 50% strain), indicating promising cushioning behaviour. They also show a low compression set, indicating promising sealability. Overall, the work here provides a step towards using protein biofoams as a sustainable alternative to fossil-based plastic/rubber foams in applications where absorbent and/or mechanical properties play a key role.
Pre-collapse spaceborne deformation monitoring of the Kakhovka dam, Ukraine, from 2017 to 2023
Authors: Tavakkoliestahbanati Amin , Milillo Pietro , Kuai Hao , Giardina Giorgia 
Published: April 2024

AbstractThe Kakhovka Dam on the Dnieper River in Kherson Oblast, Ukraine, was completed in 1956 as the final dam in the Dnieper reservoir cascade. On the morning of June 6th, 2023, a substantial portion of the dam suffered a collapse while under Russian control. This incident was documented through satellite optical and radar images, providing valuable evidence of the dam’s condition. Here we present the results of multi-temporal Interferometric Synthetic Aperture Radar (MT-InSAR) monitoring of the Kakhovka dam. The dam is vital for water management and hydroelectric power generation. Utilizing multi-temporal InSAR (MT-InSAR) data, we assessed the dam deformations prior to the collapse. Our findings indicate movements of the south side, facing the Dniprovska Gulf, compatible with several possible damage mechanisms. This study highlights the significance of employing spaceborne advanced monitoring techniques to detect signs of distress and ensure the stability of critical infrastructure.
California dominates U.S. emissions of the pesticide and potent greenhouse gas sulfuryl fluoride
Authors: Gaeta Dylan C. , Mühle Jens , Vimont Isaac J. , Crotwell Molly , Hu Lei , Miller John B. , McKain Kathryn , Baier Bianca C. , Zhang Mingyang , Bao Jianing , Miller Benjamin R. , Miller Scot M. 
Published: April 2024

AbstractSulfuryl fluoride (SO2F2) is a synthetic pesticide and a potent greenhouse gas that is accumulating in the global atmosphere. Rising emissions are a concern since SO2F2 has a relatively long atmospheric lifetime and a high global warming potential. The U.S. is thought to contribute substantially to global SO2F2 emissions, but there is a paucity of information on how emissions of SO2F2 are distributed across the U.S., and there is currently no inventory of SO2F2 emissions for the U.S. or individual states. Here we provide an atmospheric measurement-based estimate of U.S. SO2F2 emissions using high-precision SO2F2 measurements from the NOAA Global Greenhouse Gas Reference Network (GGGRN) and a geostatistical inverse model. We find that California has the largest SO2F2 emissions among all U.S. states, with the highest emissions from southern coastal California (Los Angeles, Orange, and San Diego counties). Outside of California, only very small and infrequent SO2F2 emissions are detected by our analysis of GGGRN data. We find that California emits 60-85% of U.S. SO2F2 emissions, at a rate of 0.26 ( ± 0.10) Gg yr−1. We estimate that emissions of SO2F2 from California are equal to 5.5–12% of global SO2F2 emissions.
Millennial-aged pyrogenic carbon in high-latitude mineral soils
Authors: Schiedung Marcus , Ascough Philippa , Bellè Severin-Luca , Bird Michael I. , Bröder Lisa , Haghipour Negar , Hilton Robert G. , Lattaud Julie , Abiven Samuel 
Published: April 2024

AbstractWildfires in the Arctic are producing pyrogenic carbon as product of incomplete biomass combustion. The storage and distribution of pyrogenic carbon in soils is poorly known, especially in carbon rich permafrost-affected mineral soils. Here, we extracted pyrogenic carbon in mineral soils from eleven forest sites across the North Canadian permafrost regions by hydrogen pyrolysis. We found pyrogenic carbon with millennial-scale ages that were older in continuous (1960–12,690 calibrated years before present) than in discontinuous (510–3560 calibrated years before present) permafrost-affected soils. In all cases, pyrogenic carbon showed longer residence times compared to bulk soil organic carbon. The proportions of pyrogenic carbon on total soil organic carbon were consistent at 6.9 ± 0.5% of total soil organic carbon. Thus, pyrogenic carbon forms a significant component of the total soil organic carbon and climatic as well as soil factors control the long residence times of pyrogenic carbon in vulnerable high-latitude forest mineral soils.
Immunogenicity and efficacy of VLA2001 vaccine against SARS-CoV-2 infection in male cynomolgus macaques
Authors: Galhaut Mathilde , Lundberg Urban , Marlin Romain , Schlegl Robert , Seidel Stefan , Bartuschka Ursula , Heindl-Wruss Jürgen , Relouzat Francis , Langlois Sébastien , Dereuddre-Bosquet Nathalie , Morin Julie , Galpin-Lebreau Maxence , Gallouët Anne-Sophie , Gros Wesley , Naninck Thibaut , Pascal Quentin , Chapon Catherine , Mouchain Karine , Fichet Guillaume , Lemaitre Julien , Cavarelli Mariangela , Contreras Vanessa , Legrand Nicolas , Meinke Andreas , Le Grand Roger 
Published: April 2024

Abstract
Background
The fight against COVID-19 requires mass vaccination strategies, and vaccines inducing durable cross-protective responses are still needed. Inactivated vaccines have proven lasting efficacy against many pathogens and good safety records. They contain multiple protein antigens that may improve response breadth and can be easily adapted every year to maintain preparedness for future seasonally emerging variants.

Methods
The vaccine dose was determined using ELISA and pseudoviral particle-based neutralization assay in the mice. The immunogenicity was assessed in the non-human primates with multiplex ELISA, neutralization assays, ELISpot and intracellular staining. The efficacy was demonstrated by viral quantification in fluids using RT-qPCR and respiratory tissue lesions evaluation.

Results
Here we report the immunogenicity and efficacy of VLA2001 in animal models. VLA2001 formulated with alum and the TLR9 agonist CpG 1018™ adjuvant generate a Th1-biased immune response and serum neutralizing antibodies in female BALB/c mice. In male cynomolgus macaques, two injections of VLA2001 are sufficient to induce specific and polyfunctional CD4+ T cell responses, predominantly Th1-biased, and high levels of antibodies neutralizing SARS-CoV-2 infection in cell culture. These antibodies also inhibit the binding of the Spike protein to human ACE2 receptor of several variants of concern most resistant to neutralization. After exposure to a high dose of homologous SARS-CoV-2, vaccinated groups exhibit significant levels of protection from viral replication in the upper and lower respiratory tracts and from lung tissue inflammation.

Conclusions
We demonstrate that the VLA2001 adjuvanted vaccine is immunogenic both in mouse and NHP models and prevent cynomolgus macaques from the viruses responsible of COVID-19.
Meso-scale seabed quantification with geoacoustic inversion
Authors: Sonnemann Tim , Dettmer Jan , Holland Charles W. , Dosso Stan E. 
Published: April 2024

AbstractKnowledge of sub-seabed geoacoustic properties, for example depth dependent sound speed and porosity, is of importance for a variety of applications. Here, we present a semi-automated geoacoustic inversion method for autonomous underwater vehicle data that objectively adapts model inference to seabed structure. Through parallelized trans-dimensional Bayesian inference, we infer seabed properties along a 12 km survey track on the scale of about 10 cm and 50 m in the vertical and horizontal, respectively. The inferred seabed properties include sound speed, attenuation, density, and porosity as a function of depth from acoustic reflection coefficient data. Parameter uncertainties are quantified, and the seabed properties agree closely with core samples at two control points and the layering structure with an independent sub-bottom seismic survey. Recovering high resolution seabed properties over large areas is shown to be feasible, which could become an important tool for marine industries, navies and oceanic research organizations.
National commitments to Aichi Targets and their implications for monitoring the Kunming-Montreal Global Biodiversity Framework
Authors: Maney Calum , Guaras Daniela , Harrison Jerry , Guizar-Coutiño Alejandro , Harfoot Michael B. J. , Hill Samantha L. L. , Burgess Neil D. , Sutherland William 
Published: April 2024

AbstractThe Convention on Biological Biodiversity (CBD) exists as a major multilateral environmental agreement to safeguard biodiversity and “live in harmony with nature”. To deliver it, strategies and frameworks are set out in regular agreements that are then implemented at the national scale. However, we are not on track to achieve overall goals, and frameworks so far have not been successful. This could be due to unambitious targets, low follow-through on commitments, or desired outcomes for nature not being achieved when action is taken. Here, we focus on national planning and reporting documents from a set of 30% of Parties to the CBD. We found that nearly half of the commitments mentioned in national planning documents did not appear in the Sixth National Reports and that further losses emerged due to measures reported as incomplete or ineffective. There were differences between commitments to each of the Aichi Targets, with more losses in high-profile and “institutionally challenging” Targets. Commitments from Parties in different Human Development Index categories had different outcomes among Targets, and Parties self-identifying as “megadiverse countries” had overall higher rates of reported success. Our results are important for informing the monitoring of commitment implementation in the Kunming-Montreal “global biodiversity package”.
Metabolic reprogramming of the retinal pigment epithelium by cytokines associated with age-related macular degeneration
Authors: Hansman David S. , Ma Yuefang , Thomas Daniel , Smith Justine R. , Casson Robert J. , Peet Daniel J. 
Published: April 2024

Abstract
The complex metabolic relationship between the retinal pigment epithelium (RPE) and photoreceptors is essential for maintaining retinal health. Recent evidence indicates the RPE acts as an adjacent lactate sink, suppressing glycolysis in the epithelium in order to maximize glycolysis in the photoreceptors. Dysregulated metabolism within the RPE has been implicated in the pathogenesis of age-related macular degeneration (AMD), a leading cause of vision loss. In the present study, we investigate the effects of four cytokines associated with AMD, TNFα, TGF-β2, IL-6, and IL-1β, as well as a cocktail containing all four cytokines, on RPE metabolism using ARPE-19 cells, primary human RPE cells, and ex vivo rat eyecups. Strikingly, we found cytokine-specific changes in numerous metabolic markers including lactate production, glucose consumption, extracellular acidification rate, and oxygen consumption rate accompanied by increases in total mitochondrial volume and ATP production. Together, all four cytokines could potently override the constitutive suppression of glycolysis in the RPE, through a mechanism independent of PI3K/AKT, MEK/ERK, or NF-κB. Finally, we observed changes in glycolytic gene expression with cytokine treatment, including in lactate dehydrogenase subunit and glucose transporter expression. Our findings provide new insights into the metabolic changes in the RPE under inflammatory conditions and highlight potential therapeutic targets for AMD.
The radiologist as a physician – artificial intelligence as a way to overcome tension between the patient, technology, and referring physicians – a narrative review
Authors: Stueckle Christoph Alexander , Haage Patrick 
Published: April 2024

Background Large volumes of data increasing over time lead to a shortage of radiologistsʼ time. The use of systems based on artificial intelligence (AI) offers opportunities to relieve the burden on radiologists. The AI systems are usually optimized for a radiological area. Radiologists must understand the basic features of its technical function in order to be able to assess the weaknesses and possible errors of the system and use the strengths of the system. This “explainability” creates trust in an AI system and shows its limits.
Method Based on an expanded Medline search for the key words “radiology, artificial intelligence, referring physician interaction, patient interaction, job satisfaction, communication of findings, expectations”, subjective additional relevant articles were considered for this narrative review.
Results The use of AI is well advanced, especially in radiology. The programmer should provide the radiologist with clear explanations as to how the system works. All systems on the market have strengths and weaknesses. Some of the optimizations are unintentionally specific, as they are often adapted too precisely to a certain environment that often does not exist in practice – this is known as “overfitting”. It should also be noted that there are specific weak points in the systems, so-called “adversarial examples”, which lead to fatal misdiagnoses by the AI even though these cannot be visually distinguished from an unremarkable finding by the radiologist. The user must know which diseases the system is trained for, which organ systems are recognized and taken into account by the AI, and, accordingly, which are not properly assessed. This means that the user can and must critically review the results and adjust the findings if necessary. Correctly applied AI can result in a time savings for the radiologist. If he knows how the system works, he only has to spend a short amount of time checking the results. The time saved can be used for communication with patients and referring physicians and thus contribute to higher job satisfaction.
Conclusion Radiology is a constantly evolving specialty with enormous responsibility, as radiologists often make the diagnosis to be treated. AI-supported systems should be used consistently to provide relief and support. Radiologists need to know the strengths, weaknesses, and areas of application of these AI systems in order to save time. The time gained can be used for communication with patients and referring physicians.
Key Points
Citation Format
Patient Perspectives on Outpatient versus Inpatient Cervical Ripening for Induction of Labor
Authors: Watters Amber , Ekpe Etoroabasi , Okafor Annette , Donelan Emily 
Published: April 2024

Objective Our objective was to compare patient perceived control and experience with outpatient versus inpatient cervical ripening.
Study Design This is a retrospective mixed-methods analysis of a quality improvement initiative focused on the impact to patients of incorporating outpatient cervical ripening into routine practice. Postpartum inpatients who had elected for outpatient cervical ripening (outpatients) and those who met criteria for outpatient cervical ripening but opted for an inpatient setting (inpatients) were invited to participate in the study. Patients completed the Perceived Control in Childbirth Scale, and scores were compared between outpatient and inpatient groups using Mann–Whitney U test. In addition, semistructured questions elicited feedback prior to hospital discharge, and these qualitative data were analyzed using iterative thematic analysis.
Results The study population consisted of 36 outpatients and 38 inpatients. The median score on the Perceived Control in Childbirth Scale was 69 for outpatients and 67 for inpatients (p-value = 0.49), out of a maximum score of 72 (representing the highest level of perceived control). Both groups reported similarly high levels of perceived control, regardless of cervical ripening setting. In the qualitative analysis, pain was the most common theme in both groups. Inpatients reported more distress despite access to stronger pain medications. Outpatients utilized a variety of distraction techniques and expressed gratitude for their setting more than inpatients.
Conclusion Outpatient cervical ripening can be a patient-centered solution to obstetric throughput challenges arising from increased numbers of inductions. Those who underwent outpatient cervical ripening had similar perceived control to those who underwent inpatient cervical ripening, suggesting that individual patient preferences are most important in determining the optimal setting for care. The patients' reported experiences identified focus areas for process improvement efforts and future research, including improving patient education regarding expectations and innovating new pain management strategies for cervical ripening.
Key Points
Comparing Fetal Ultrasound Biometric Measurements to Neonatal Anthropometry at the Extremes of Birth Weight
Authors: Gleason Jessica L. , Hediger Mary L. , Chen Zhen , Grewal Jagteshwar , Newman Roger , Grobman William A. , Owen John , Grantz Katherine L. 
Published: April 2024

Objective Error in birthweight prediction by sonographic estimated fetal weight (EFW) has clinical implications, such as avoidable cesarean or misclassification of fetal risk in labor. We aimed to evaluate optimal timing of ultrasound and which fetal measurements contribute to error in fetal ultrasound estimations of birth size at the extremes of birthweight.
Study Design We compared differences in head circumference (HC), abdominal circumference (AC), femur length, and EFW between ultrasound and corresponding birth measurements within 14 (n = 1,290) and 7 (n = 617) days of birth for small- (SGA, <10th percentile), appropriate- (AGA, 10th–90th), and large-for-gestational age (LGA, >90th) newborns.
Results Average differences between EFW and birthweight for SGA neonates were: −40.2 g (confidence interval [CI]: −82.1, 1.6) at 14 days versus 13.6 g (CI: −52.4, 79.7) at 7 days; for AGA, −122.4 g (−139.6, −105.1) at 14 days versus −27.2 g (−50.4, −4.0) at 7 days; and for LGA, −242.8 g (−306.5, −179.1) at 14 days versus −72.1 g (−152.0, 7.9) at 7 days. Differences between fetal and neonatal HC were larger at 14 versus 7 days, and similar to patterns for EFW and birthweight, differences were the largest for LGA at both intervals. In contrast, differences between fetal and neonatal AC were larger at 7 versus 14 days, suggesting larger error in AC estimation closer to birth.
Conclusion Using a standardized ultrasound protocol, SGA neonates had ultrasound measurements closer to actual birth measurements compared with AGA or LGA neonates. LGA neonates had the largest differences between fetal and neonatal size, with measurements 14 days from delivery showing 3- to 4-fold greater differences from birthweight. Differences in EFW and birthweight may not be explained by a single fetal measurement; whether estimation may be improved by incorporation of other knowable factors should be evaluated in future research.
Key Points