bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Process of Care Management Following Brain Injury: A Grounded Theory Study
Authors: McCluskey Annie , Johnson Maree , Tate Robyn 
Published: February 2008

AbstractEstablishing a viable program of care and support for people with severe brain injury is challenging for professionals and family members. The aims of this grounded theory study were to explore how decisions about care were made following brain injury when an individual had received third party compensation, and factors or conditions that influenced care. The sample (n = 51) included 14 people with traumatic brain injury (mean age 36.5 years, range 19–56; mean time post-injury 9.9 years, range 2.5–37), and 37 ‘others’, who either provided or coordinated care (12 family members, 14 paid carers, 6 case managers, 4 directors of care agencies and 1 estate manager). Data were collected using single interviews with each participant. All 14 people with brain injury had received 24-hour support at the time of hospital discharge; at follow-up, only 8 received 24-hour support. Care decision-making and care maintenance occurred as an iterative process. Participants made decisions about the best living situation, configuration of carers and level of care, and then strived to maintain stable care. Care decision-making involved three key strategies (gathering information, appraising alternatives, and enacting a decision). Care maintenance also involved three key strategies (monitoring, managing conflict and supporting carers). Mutual risk tolerance and the presence of a case manager were necessary conditions for good care management, and enabled a person with brain injury to spend time alone, take low-level risks and experience autonomy. Professionals and family carers can use the findings of this study to actively increase risk taking and autonomy.
Council of Australian Governments' (COAG) Initiative for Young People With Disability in Residential Aged Care: What Are the Issues for Acquired Brain Injury?
Authors: Foster Michele , Fleming Jennifer , Tilse Cheryl 
Published: February 2008

AbstractPeople surviving severe acquired brain injury (ABI) may potentially benefit from the Council of Australian Governments' (COAG) 5-year initiative for young people with disability in residential aged-care facilities. Yet critical examination of this policy initiative for ABI population is warranted for 2 reasons. First, reliance on the disability sector to resolve the complexities of long-term care for people with ABI detracts attention from systemic failures at the health/disability sector interface, and notably, debate concerning the role of, and right to rehabilitation. Second, the COAG initiative is being pursued within an extraordinarily complex and variable contemporary care environment, involving multiple services and sectors, and historically, high unmet need. This raises questions as to the adequacy and sustainability of care provided under the responsibility of state-based disability services. In this article, it is argued that long-term care for young people with severe ABI is better served by incorporating a health and rehabilitation perspective alongside a disability support approach. Although the effectiveness of rehabilitation may be contested in some instances of very severe ABI, nevertheless the role of rehabilitation in seeking to reduce the number of young people at risk of entering residential aged care needs to be addressed in policy solutions. It is also suggested that provision of long time care in the contemporary care environment involves a number of challenges due to the complex and changing patterns of need, diverse funding arrangements and mix of government and nongovernment services, and the increasing demand for care.
The Importance of Reflexive Practice Within the Context of Life-Long Career Development for Rehabilitation Counsellors
Authors: Parks Richard 
Published: February 2008

AbstractWhere reflexivity is the process of ‘being aware in the moment of what is influencing our internal and external responses’ (Etherington, 2004), this literature review was aimed at exploring the extent to which rehabilitation counsellors could use the core competencies outlined by the Australian Society of Rehabilitation Counsellors (ASORC) for their own career development. Reference was given to two specific competencies: (1) Vocational counselling, and (2) Counselling and interpersonal skills to not only determine whether the espoused skills in these areas could be used to advance career development but to also address the issue of compassion fatigue. In addition to these competencies, the role of the organisation was also brought into question regarding its influences on the career development for rehabilitation counsellors and the extent it to which it can minimise burnout.
The Leuven Longitudinal Twin Study (LLTS): Major Findings
Authors: Beunen Gaston P. , Peeters Maarten W. , Maes Hermine H. , Loos Ruth J. F. , Claessens Albrecht L. , Derom Catherine , Vlietinck Robert , Thomis Martine A. 
Published: February 2008

AbstractAlongitudinal study of growth and physical fitness of twins and their parents was designed in 1985. The major aims of this Leuven Longitudinal Twin Study were to quantify the genetic and environmental determination of (1) somatic characteristics, biological maturation and physical performance characteristics during the growth process, (2) the growth and developmental patterns, and (3) the covariation in somatic and performance characteristics.
Characteristics of Spontaneously Conceived Twins Versus Twins After Assisted Conception
Authors: Delbaere Ilse , Verstraelen Hans , Temmerman Marleen 
Published: February 2008

AbstractThe East Flanders Prospective Twin Survey comprises data concerning placentation, mode of conception and perinatal outcome. As such, this database is convenient to study differences between spontaneous twins and twins resulting from assisted reproductive technologies (ART). Papers based on this survey demonstrated higher incidences of velamentous cord insertion, single umbilical artery and preterm birth in twins after ART.
Timing of Twinning, X-Inactivation and Sex Proportion at Birth
Authors: Derom Catherine , Vlietinck Robert , Derom Robert 
Published: February 2008

AbstractTwins are not a homogeneous group. According to zygosity and chorionicity essential differences exist. The lower sex proportion at birth is due to the monozygotic twins, especially the monoamnionic variety. X-inactivation patterns in monozygotic twin girls are totally symmetrical in monoamnionic pairs, almost symmetrical in monochorionic diamnionic pairs and can be very asymmetrical in the dichorionic variety.
Clustering of Genetically Defined Allele Classes in the Caenorhabditis elegans DAF-2 Insulin/IGF-1 Receptor
Authors: Patel Dhaval S , Garza-Garcia Acely , Nanji Manoj , McElwee Joshua J , Ackerman Daniel , Driscoll Paul C , Gems David 
Published: February 2008

Abstract
The DAF-2 insulin/IGF-1 receptor regulates development, metabolism, and aging in the nematode Caenorhabditis elegans. However, complex differences among daf-2 alleles complicate analysis of this gene. We have employed epistasis analysis, transcript profile analysis, mutant sequence analysis, and homology modeling of mutant receptors to understand this complexity. We define an allelic series of nonconditional daf-2 mutants, including nonsense and deletion alleles, and a putative null allele, m65. The most severe daf-2 alleles show incomplete suppression by daf-18(0) and daf-16(0) and have a range of effects on early development. Among weaker daf-2 alleles there exist distinct mutant classes that differ in epistatic interactions with mutations in other genes. Mutant sequence analysis (including 11 newly sequenced alleles) reveals that class 1 mutant lesions lie only in certain extracellular regions of the receptor, while class 2 (pleiotropic) and nonconditional missense mutants have lesions only in the ligand-binding pocket of the receptor ectodomain or the tyrosine kinase domain. Effects of equivalent mutations on the human insulin receptor suggest an altered balance of intracellular signaling in class 2 alleles. These studies consolidate and extend our understanding of the complex genetics of daf-2 and its underlying molecular biology.
Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae
Authors: Kelleher Danielle F , de Carvalho Carlos Egydio , Doty Alana V , Layton Marnie , Cheng Andy T , Mathies Laura D , Pilgrim Dave , Haag Eric S 
Published: February 2008

Abstract
The nematodes Caenorhabditis elegans and C. briggsae independently evolved self-fertile hermaphroditism from gonochoristic ancestors. C. briggsae has variably divergent orthologs of nearly all genes in the C. elegans sex determination pathway. Their functional characterization has generally relied on reverse genetic approaches, such as RNA interference and cross-species transgene rescue and more recently on deletion mutations. We have taken an unbiased forward mutagenesis approach to isolating zygotic mutations that masculinize all tissues of C. briggsae hermaphrodites. The screens identified loss-of-function mutations in the C. briggsae orthologs of tra-1, tra-2, and tra-3. The somatic and germline phenotypes of these mutations are largely identical to those of their C. elegans homologs, including the poorly understood germline feminization of tra-1(lf) males. This overall conservation of Cb-tra phenotypes is in contrast to the fem genes, with which they directly interact and which are significantly divergent in germline function. In addition, we show that in both C. briggsae and C. elegans large C-terminal truncations of TRA-1 that retain the DNA-binding domain affect sex determination more strongly than somatic gonad development. Beyond these immediate results, this collection of mutations provides an essential foundation for further comparative genetic analysis of the Caenorhabditis sex determination pathway.
Recombination Map of the Common Shrew,Sorex araneus(Eulipotyphla, Mammalia)
Authors: Borodin Pavel M , Karamysheva Tatyana V , Belonogova Nadezhda M , Torgasheva Anna A , Rubtsov Nikolai B , Searle Jeremy B 
Published: February 2008

AbstractThe Eurasian common shrew (Sorex araneus L.) is characterized by spectacular chromosomal variation, both autosomal variation of the Robertsonian type and an XX/XY1Y2 system of sex determination. It is an important mammalian model of chromosomal and genome evolution as it is one of the few species with a complete genome sequence. Here we generate a high-precision cytological recombination map for the species, the third such map produced in mammals, following those for humans and house mice. We prepared synaptonemal complex (SC) spreads of meiotic chromosomes from 638 spermatocytes of 22 males of nine different Robertsonian karyotypes, identifying each autosome arm by differential DAPI staining. Altogether we mapped 13,983 recombination sites along 7095 individual autosomes, using immunolocalization of MLH1, a mismatch repair protein marking recombination sites. We estimated the total recombination length of the shrew genome as 1145 cM. The majority of bivalents showed a high recombination frequency near the telomeres and a low frequency near the centromeres. The distances between MLH1 foci were consistent with crossover interference both within chromosome arms and across the centromere in metacentric bivalents. The pattern of recombination along a chromosome arm was a function of its length, interference, and centromere and telomere effects. The specific DNA sequence must also be important because chromosome arms of the same length differed substantially in their recombination pattern. These features of recombination show great similarity with humans and mice and suggest generality among mammals. However, contrary to a widespread perception, the metacentric bivalent tu usually lacked an MLH1 focus on one of its chromosome arms, arguing against a minimum requirement of one chiasma per chromosome arm for correct segregation. With regard to autosomal chromosomal variation, the chromosomes showing Robertsonian polymorphism display MLH1 foci that become increasingly distal when comparing acrocentric homozygotes, heterozygotes, and metacentric homozygotes. Within the sex trivalent XY1Y2, the autosomal part of the complex behaves similarly to other autosomes.
Identification of Motifs That Are Conserved in 12 Drosophila Species and Regulate Midline Glia vs. Neuron Expression
Authors: Estes Patricia , Fulkerson Eric , Zhang Yi 
Published: February 2008

Abstract
Functional complexity of the central nervous system (CNS) is reflected by the large number and diversity of genes expressed in its many different cell types. Understanding the control of gene expression within cells of the CNS will help reveal how various neurons and glia develop and function. Midline cells of Drosophila differentiate into glial cells and several types of neurons and also serve as a signaling center for surrounding tissues. Here, we examine regulation of the midline gene, wrapper, required for both neuron–glia interactions and viability of midline glia. We identify a region upstream of wrapper required for midline expression that is highly conserved (87%) between 12 Drosophila species. Site-directed mutagenesis identifies four motifs necessary for midline glial expression: (1) a Single-minded/Tango binding site, (2) a motif resembling a pointed binding site, (3) a motif resembling a Sox binding site, and (4) a novel motif. An additional highly conserved 27 bp are required to restrict expression to midline glia and exclude it from midline neurons. These results suggest short, highly conserved genomic sequences flanking Drosophila midline genes are indicative of functional regulatory regions and that small changes within these sequences can alter the expression pattern of a gene.
Genetic Interactions of the Aspergillus nidulans atmAATM Homolog With Different Components of the DNA Damage Response Pathway
Authors: Malavazi Iran , Lima Joel Fernandes , de Castro Patrícia Alves , Savoldi Marcela , de Souza Goldman Maria Helena , Goldman Gustavo Henrique 
Published: February 2008

Abstract
Ataxia telangiectasia mutated (ATM) is a phosphatidyl-3-kinase-related protein kinase that functions as a central regulator of the DNA damage response in eukaryotic cells. In humans, mutations in ATM cause the devastating neurodegenerative disease ataxia telangiectasia. Previously, we characterized the homolog of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we found that AtmA is also required for polarized hyphal growth. Here, we extended these studies by investigating which components of the DNA damage response pathway are interacting with AtmA. The AtmAATM loss of function caused synthetic lethality when combined with mutation in UvsBATR. Our results suggest that AtmA and UvsB are interacting and they are probably partially redundant in terms of DNA damage sensing and/or repairing and polar growth. We identified and inactivated A. nidulans chkACHK1 and chkBCHK2 genes. These genes are also redundantly involved in A. nidulans DNA damage response. We constructed several combinations of double mutants for ΔatmA, ΔuvsB, ΔchkA, and ΔchkB. We observed a complex genetic relationship with these mutations during the DNA replication checkpoint and DNA damage response. Finally, we observed epistatic and synergistic interactions between AtmA, and bimEAPC1, ankAWEE1 and the cdc2-related kinase npkA, at S-phase checkpoint and in response to DNA-damaging agents.
Dynamic Regulation of Single-Stranded Telomeres in Saccharomyces cerevisiae
Authors: Smith Stephanie , Banerjee Soma , Rilo Regina , Myung Kyungjae 
Published: February 2008

Abstract
The temperature-sensitive phenotypes of yku70Δ and yku80Δ have provided a useful tool for understanding telomere homeostasis. Mutating the helicase domain of the telomerase inhibitor Pif1 resulted in the inactivation of cell cycle checkpoints and the subsequent rescue of temperature sensitivity of the yku70Δ strain. The inactivation of Pif1 in yku70Δ increased overall telomere length. However, the long G-rich, single-stranded overhangs at the telomeres, which are the major cause of temperature sensitivity, were slightly increased. Interestingly, the rescue of temperature sensitivity in strains having both pif1-m2 and yku70Δ mutations depended on the homologous recombination pathway. Furthermore, the BLM/WRN helicase yeast homolog Sgs1 exacerbated the temperature sensitivity of the yku70Δ strain. Therefore, the yKu70-80 heterodimer and telomerase maintain telomere size, and the helicase activity of Pif1 likely also helps to balance the overall size of telomeres and G-rich, single-stranded overhangs in wild-type cells by regulating telomere protein homeostasis. However, the absence of yKu70 may provide other proteins such as those involved in homologous recombination, Sgs1, or Pif1 additional access to G-rich, single-stranded DNA and may determine telomere size, cell cycle checkpoint activation, and, ultimately, temperature sensitivity.
The mob as tumor suppressor Gene Is Essential for Early Development and Regulates Tissue Growth in Drosophila
Authors: Shimizu Takeshi , Ho Li-Lun , Lai Zhi-Chun 
Published: February 2008

Abstract
Studies in Drosophila have defined a new growth inhibitory pathway mediated by Fat (Ft), Merlin (Mer), Expanded (Ex), Hippo (Hpo), Salvador (Sav)/Shar-pei, Warts (Wts)/Large tumor suppressor (Lats), and Mob as tumor suppressor (Mats), which are all evolutionarily conserved in vertebrate animals. We previously found that the Mob family protein Mats functions as a coactivator of Wts kinase. Here we show that mats is essential for early development and is required for proper chromosomal segregation in developing embryos. Mats is expressed at low levels ubiquitously, which is consistent with the role of Mats as a general growth regulator. Like mammalian Mats, Drosophila Mats colocalizes with Wts/Lats kinase and cyclin E proteins at the centrosome. This raises the possibility that Mats may function together with Wts/Lats to regulate cyclin E activity in the centrosome for mitotic control. While Hpo/Wts signaling has been implicated in the control of cyclin E and diap1 expression, we found that it also modulates the expression of cyclin A and cyclin B. Although mats depletion leads to aberrant mitoses, this does not seem to be due to compromised mitotic spindle checkpoint function.
Active Miniature Transposons From a Plant Genome and Its Nonrecombining Y Chromosome
Authors: Bergero R , Forrest A , Charlesworth D 
Published: February 2008

AbstractMechanisms involved in eroding fitness of evolving Y chromosomes have been the focus of much theoretical and empirical work. Evolving Y chromosomes are expected to accumulate transposable elements (TEs), but it is not known whether such accumulation contributes to their genetic degeneration. Among TEs, miniature inverted-repeat transposable elements are nonautonomous DNA transposons, often inserted in introns and untranslated regions of genes. Thus, if they invade Y-linked genes and selection against their insertion is ineffective, they could contribute to genetic degeneration of evolving Y chromosomes. Here, we examine the population dynamics of active MITEs in the young Y chromosomes of the plant Silene latifolia and compare their distribution with those in recombining genomic regions. To isolate active MITEs, we developed a straightforward approach on the basis of the assumption that recent transposon insertions or excisions create singleton or low-frequency size polymorphisms that can be detected in alleles from natural populations. Transposon display was then used to infer the distribution of MITE insertion frequencies. The overall frequency spectrum showed an excess of singleton and low-frequency insertions, which suggests that these elements are readily removed from recombining chromosomes. In contrast, insertions on the Y chromosomes were present at high frequencies. Their potential contribution to Y degeneration is discussed.
Metabolic Changes Associated With Adaptive Diversification in Escherichia coli
Authors: Le Gac Mickaël , Brazas Michelle D , Bertrand Melanie , Tyerman Jabus G , Spencer Christine C , Hancock Robert E W , Doebeli Michael 
Published: February 2008

Abstract
During a 1000-generation evolution experiment, two types of morphologically and kinetically distinct bacteria repeatedly diverged from a common ancestor in a fully sympatric seasonal environment containing glucose and acetate. To investigate the metabolic modifications associated with this adaptive diversification, we compared transcription profiles of the two derived types and the common ancestor. Both derived types share a suite of common metabolic changes that may represent adaptation to the environment preceding the diversification event. These include improved translation efficiency, glucose uptake capacity via the mal/lamB genes, upregulation of various transporters during stationary phase, and likely the disruption of the rbs operon. The diversification event is associated with the overexpression of genes involved in the TCA cycle, glyoxylate shunt, acetate consumption, and anaerobic respiration in one type and in acetate excretion in the other. These results reveal that competition for both carbon and oxygen have likely played an important role in the adaptation of Escherichia coli during this adaptive diversification event, where one derived type mainly consumes glucose at a fast rate when oxygen is not limiting, and the other derived type consumes glucose and acetate at a slower rate, even when oxygen is limiting.
The Conserved Proteins CHE-12 and DYF-11 Are Required for Sensory Cilium Function in Caenorhabditis elegans
Authors: Bacaj Taulant , Lu Yun , Shaham Shai 
Published: February 2008

Abstract
Sensory neuron cilia are evolutionarily conserved dendritic appendages that convert environmental stimuli into neuronal activity. Although several cilia components are known, the functions of many remain uncharacterized. Furthermore, the basis of morphological and functional differences between cilia remains largely unexplored. To understand the molecular basis of cilia morphogenesis and function, we studied the Caenorhabditis elegans mutants che-12 and dyf-11. These mutants fail to concentrate lipophilic dyes from their surroundings in sensory neurons and are chemotaxis defective. In che-12 mutants, sensory neuron cilia lack distal segments, while in dyf-11 animals, medial and distal segments are absent. CHE-12 and DYF-11 are conserved ciliary proteins that function cell-autonomously and are continuously required for maintenance of cilium morphology and function. CHE-12, composed primarily of HEAT repeats, may not be part of the intraflagellar transport (IFT) complex and is not required for the localization of some IFT components. DYF-11 undergoes IFT-like movement and may function at an early stage of IFT-B particle assembly. Intriguingly, while DYF-11 is expressed in all C. elegans ciliated neurons, CHE-12 expression is restricted to some amphid sensory neurons, suggesting a specific role in these neurons. Our results provide insight into general and neuron-specific aspects of cilium development and function.