bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ablative Radioembolization (SIRT) of a Large Inoperable Renal Cell Carcinoma Invading the Liver That Resulted in Hematuria Control and Complete Necrosis by mRECIST Criteria
Authors: Spyridonidis Ioannis , Dimopoulos Platon , Spyridonidis Trifon , Christopoulou Athina , Katsanos Konstantinos 
Published: April 2024

Abstract
We report the successful application of radioembolization (SIRT) in a 77-year-old man with end-stage renal disease on hemodialysis and repeated episodes of macroscopic hematuria due to a large renal cell carcinoma of the right kidney extending to liver segment VI. A compassionate SIRT therapy was performed with resin microspheres through the upper pole renal artery and the feeding segmental artery of liver segment VI. Hematuria was resolved after treatment, and 4 months later, a follow-up CT scan revealed tumor size reduction and complete tumor necrosis (Response Evaluation Criteria in Solid Tumors criteria). Ablative SIRT therapy could be a safe and efficient option in a large inoperable RCC.
Radioiodine Uptake at Suture Site Granuloma Mimicking Neck Node Metastasis on 131I Whole-Body Iodine Scintigraphy
Authors: Singh Parneet , Agrawal Kanhaiyalal , Patel Ranjan Kumar , Patro P. Sai Sradha , Parida Girish Kumar 
Published: April 2024

Abstract
Differentiated thyroid carcinoma constitutes over 90% of all thyroid cancers. The standard treatment approach involves total or near-total thyroidectomy with or without neck dissection followed by 131I whole-body scintigraphy (WBS) to detect local or distant metastases. Radioiodine offers high sensitivity and specificity for detection of metastatic disease in well differentiated thyroid carcinoma. However, despite its high accuracy, 131I WBS demonstrates false-positive results, mostly at inflammatory or infective site. These false-positive radioiodine accumulation can lead to misdiagnosis and unwarranted radioiodine treatment. This case presents localization of 131I to the suture site granuloma leading to false-positive results on 131I WBS.
SMARCA4-Deficient Poorly Differentiated Adenocarcinoma of the Gallbladder
Authors: Koyasu Sho , Sugimoto Akihiko , Matsubara Junichi , Muto Manabu , Nakamoto Yuji 
Published: April 2024

Abstract
A 64-year-old woman presented with chest pain while eating and was referred to our hospital. Physical examination revealed abdominal distension, tenderness, and lower-extremity edema. Imaging revealed a large gallbladder tumor infiltrating the liver, with ascites and pleural effusion. A biopsy confirmed a poorly differentiated adenocarcinoma with SMARCA4 deficiency (cT3N2M1, cStage IV). Chemotherapy was ineffective and led to tumor progression. The patient died 9 months later. Recently, attention has been paid to SMARCA4 deficiency, which is a genetic mutation found in tumors. Here, we report on poorly differentiated adenocarcinomas of the gallbladder based on imaging findings, including FDG PET.
Splenic Pseudoprogression After CAR-T Therapy Detected by 18F-FDG PET/CT in a Refractory Diffuse Large B-Cell Lymphoma Patient
Authors: Yao Xilan , Wang Hongrong , Kan Ying , Wang Wei , Yang Jigang 
Published: April 2024

Abstract
A 43-year-old woman diagnosed with refractory diffuse large B-cell lymphoma was referred to chimeric antigen receptor T-cell therapy at our institution. After 3 cycles of bridging therapy, preinfusion 18F-FDG PET/CT suggested a complete metabolic response. 18F-FDG PET/CT 1 month after chimeric antigen receptor T-cell infusion showed 2 foci of elevated activity in the spleen, which was finally confirmed as pseudoprogression.
Comparison of 18F-MFBG PET/CT and 18F-FDG PET/CT Images of Metastatic Neuroblastoma
Authors: Zhang Wenqian , Liu Lei , Yuan Gengbiao , Deng Maoxue , Cai Liang 
Published: April 2024

Abstract
Two children with neuroblastoma underwent tumor resection and postoperative chemotherapy. After treatment, they participated in a clinical trial and received 18F-MFBG and 18F-FDG PET/CT examinations. Although similar lesions were found in the 2 examinations, the uptake pattern was different. The lymph nodes and bone lesions had intense 18F-MFBG activity, whereas 18F-FDG uptake was not very impressive. The uptake of bone marrow by 18F-MFBG was significantly stronger than that by 18F-FDG. This case emphasizes that 18F-MFBG PET/CT is superior to 18F-FDG PET/CT in detecting the metastases of neuroblastoma.
Prostate-Specific Membrane Antigen–Avid Neurofibroma Mimicking Cutaneous Metastasis in Metastatic Castration-Resistant Prostate Cancer on 18F-PSMA-1007 PET/CT
Authors: Aggarwal Piyush , Ambalavanan Nivetha , Sood Ashwani , Gupta Kirti , Dahiya Divya , Mittal Bhagwant Rai 
Published: April 2024

Abstract
The occurrence of cutaneous metastases in prostate cancer is exceedingly rare. Many benign lesions and nonprostatic cancers can express the prostate-specific membrane antigen (PSMA). They can potentially mimic metastasis of prostate cancer and lead to misinterpretation of PSMA PET/CT findings. Additionally, it has significant management and prognostic implications. We present a rare case of an 88-year-old man with metastatic castration-resistant prostate cancer who showed a PSMA-expressing subcutaneous nodule in the scalp on 18F-PSMA-1007 PET/CT, raising the suspicion of cutaneous metastasis. However, its biopsy revealed a neurofibroma, altering the disease prognosis and management.
Management of Maxillofacial Trauma in Pregnant Women
Authors: Taylor Ruby R. , Jabori Sinan , Kim Minji , Smartz Taylor , Hale Eva S. , Peleg Michael , Kassira Wrood , Lessard Anne-Sophie , Thaller Seth R. 
Published: April 2024

Introduction:
Trauma during pregnancy deserves special attention as management must be directed towards both the mother and the fetus. Management of maxillofacial fractures in pregnancy can adversely affect the well-being of the fetus by impinging on normal functions such as respiration, mastication, and nutrition. Pregnancy complicates the management of facial injury due to the maintenance of the patent airway, anesthesia considerations, and imaging restraints. The purpose of this study is to use three illustrative from our own institution to further elucidate education on the management of mandible fracture in pregnancy with a focus on multidisciplinary treatment and outcomes.

Methods:
A retrospective chart review was performed for all cases of facial fractures admitted to Ryder Trauma Center from 2012 to 2022. During this time, 4,910 patients presented with facial fractures 1319 patients were female. Three of the patients were pregnant at the time of admission. Demographics, mechanism of injury, associated injuries, and management information were collected.

Results:
Patient 1 was a 20-year-old female presented to Ryder Trauma Center following a motor vehicle collision. She was 17 weeks pregnant at the time of admission and was found to have a left mandibular angle fracture. Patient 2 was a 14-year-old female who presented to Ryder Trauma Center status post gunshot wound to the mandible after she and her brother were unknowingly playing with a loaded gun. She was 18 weeks pregnant at the time of admission, with a past medical history of domestic violence, suicidal ideation, and major depressive disorder. Patient 3 was a 20-year-old female 36 weeks pregnant at the time of admission. She presented with a right paraymphyseal fracture and left mandibular angle fracture as a result of falling on the stairs. Patients all underwent surgical repair of fractures.
Efficacy and Safety of Endoscopic Surgery Versus Craniotomy for Hypertensive Putamen Hemorrhage
Authors: Wang Anshuo , Sun Zikang , Zhang Wen , He Hu , Wang Fei 
Published: April 2024

Objective:
This study aimed to evaluate the efficacy and safety of neuroendoscopy for treating hypertensive putamen hemorrhage (HPH), compared with traditional craniotomy.

Methods:
We retrospectively analyzed 81 consecutive patients with HPH treated with neuroendoscopy (n=36) or craniotomy (n=45) in the Department of Neurosurgery at the Anhui Provincial Hospital Affiliated to Anhui Medical University between January 2015 and December 2017. We compared the clinical and radiographic outcomes, excluded 14 patients who did not meet the inclusion criteria. Patient characteristics in emergency room were recorded. In addition, hospitalization days, total cost during hospitalization, operative time, blood loss, evacuation rate, rebreeding, intracranial infection, pulmonary infection, epilepsy, hemorrhage of digestive tract, venous thrombus, hypoproteinemia, aphasia, oculomotor paralysis, mortality, Modified Rankin Scale score 6 months after surgery, and Glasgow Outcome Scale score 6 months after surgery were compared between the 2 groups.

Results:
Comparative analysis of preoperative patient data revealed no notable disparities. Neuroendoscopic surgery afford distinct benefits including reduced operative time, minimal patient blood loss, and enhanced efficacy in hematoma evacuation. However, the incidence of postoperative complications such as rebleeding, intracranial infections, pulmonary infections, postoperative epilepsy, hemorrhage of digestive tract, venous thrombus, hypoproteinemia, aphasia, and oculomotor paralysis did not significantly differ. In contrast, endoscopic techniques, relative to conventional craniotomy for hematoma evacuation, are characterized by less invasive incisions, a marked decrease in the duration of hospitalization, and a substantial reduction in associated healthcare costs. Furthermore, endoscopic techniques contribute to superior long-term recuperative outcomes in patients, without altering mortality rates.

Conclusions:
In comparison to the conventional method of craniotomy, the utilization of neuroendoscopy in the treatment of hypertensive putamen hemorrhage (HPH) may offer a more efficacious, minimally invasive, and cost-effective approach. This alternative approach has the potential to decrease the length of hospital stays and improve long-term neurologic outcomes, without altering mortality rates.
Assessing Healthcare Simulation Facilitation
Authors: Mossenson Adam I. , Livingston Patricia L. , Tuyishime Eugene , Brown Janie A. 
Published: April 2024

Summary Statement
Assessment tools support simulation facilitation skill development by guiding practice, structuring feedback, and promoting reflective learning among educators. This scoping review followed a systematic process to identify facilitation assessment tools used in postlicensure healthcare simulation. Secondary objectives included mapping of the validity evidence to support their use and a critical appraisal of their suitability for simulation faculty development in low-resource settings. Database searching, gray literature searching, and stakeholder engagement identified 11,568 sources for screening, of which 72 met criteria for full text review. Thirty sources met inclusion; 16 unique tools were identified. Tools exclusively originated from simulation practice in high-resource settings and predominantly focused on debriefing. Many tools have limited validity evidence supporting their use. In particular, the validity evidence supporting the extrapolation and implications of assessment is lacking. No current tool has high context suitability for use in low-resource settings.
Patient Perceptions and Experiences With Maggot Debridement Therapy for Managing Chronic Wounds
Authors: Babiarczyk Beata , Tobiczyk Joanna 
Published: April 2024

PURPOSE:
The purpose of this study was to describe patient experiences and satisfaction with use of maggot debridement therapy (MDT) for hard-to-heal wounds.

DESIGN:
Descriptive, cross-sectional study.

SUBJECTS AND SETTING:
The sample comprised 60 participants, 60% were male (n = 36). Their mean age was 62.9 (SD = 20) years. Almost half of participants had lower extremity wounds (n = 26; 43.3%), diabetic foot ulcers (n = 18; 30%), and pressure injuries (n = 9; 15%). Most received maggot therapy via biobags (n = 36; 60%).

METHODS:
Participants completed a questionnaire designed for purposes of the study that queried demographic and pertinent clinical characteristics, current health status including current topical therapies, and duration of their chronic wound. Nine items queried emotional responses prior to MDT, the amount and method of the maggot therapy, discomfort experienced during therapy, and sources of information regarding this treatment.

RESULTS:
Emotional responses before starting MDT included disgust (n = 30, 50%), anxiety (n = 26, 43.3%), doubts about its effectiveness (n =20, 33.3%), and disbelief (n = 11, 18.3%). Approximately one-third of participants reported feelings of biting, itching, and fear of the maggots. Despite these feelings, a majority (n = 38, 63.3%) indicated that they were pleased with treatment outcome and willing to undergo additional MDT if needed.

CONCLUSIONS:
While a majority of participants with nonhealing chronic wounds reported negative emotions association with MDT, more than half indicated that they were pleased with the outcome of treatment and willing to undergo repeat treatment if indicated.
Mothers’ knowledge and practices towards oral hygiene of their children aged 5-9 years old: a cross-sectional study in Dhaka
Authors: Tamannur Tahazid , Das Sadhan Kumar , Nesa Arifatun , Nahar Foijun , Nowshin Nadia , Binty Tasnim Haque , Shakil Shafiul Azam , Kundu Shuvojit Kumar , Siddik Md Abu Bakkar , Rafsun Shafkat Mahmud , Habiba Umme , Farhana Zaki , Sultana Hafiza , Kamil Anton Abdulbasah , Rahman Mohammad Meshbahur 
Published: April 2024

AbstractBackgroundHealthy oral hygiene is crucial for overall health and well-being. Parents’ dental care knowledge and practice affect their children’s oral health. Thus, this study assessed the oral hygiene knowledge and practice in mothers of children aged 5-9 years.MethodsThis cross-sectional study was conducted from 1 January to 31 December 2022 in Dhaka, Bangladesh. Mothers’ oral hygiene knowledge and practices were assessed through a semi-structured questionnaire. Statistical analysis including the Mann–Whitney U test and Kruskal– Wallis one-way ANOVA test were performed to show average knowledge and practice variations among different socio-demographics of mothers.ResultsOut of 400 samples, the mean age of mothers was 30.94±5.15 years where majority were in Muslim faith (97%), housewife (86.8%) and came from nuclear family (68.0%). The prevalence of good knowledge was 41.2%, following 21.5% had moderately average, 18.8% had average and 18.5% mothers had poor knowledge respectively on their children’s oral hygiene. On the other hand, 45.5% mothers had good practice, following 19.5% had average practice, 18.8% had moderately average and 16.2% had poor practice behavior. Mothers’ knowledge levels were significantly (p<0.05) associated with age, education, family size, and monthly income. On the other hand, educational status and income was significantly (p<0.05) associated with mothers’ oral hygiene practices. Non-parametric analysis revealed that the average knowledge level was significantly (p<0.05) higher with respect to higher age group, educational attainment, currently working status, and whose family income was high. On the other hand, oral hygiene practice level was significantly (p<0.05) higher among mother having higher education and higher family income. Mothers’ knowledge was significantly (p<0.05) and positively correlated with the practice behavior obtained by Pearson correlation coefficient.ConclusionsThe revealed that the knowledge and practices of mothers directly influence the oral hygiene behavior of children. Mothers with sound knowledge tend to exhibit positive practices concerning their children’s oral hygiene. These findings underscore the importance of taking necessary actions to enhance both the knowledge and practices related to oral hygiene among mothers, thereby ensuring the well-being of their children.
CELEBRIMBOR: Pangenomes from metagenomes
Authors: Hellewell Joel , Horsfield Samuel T. , von Wachsmann Johanna , Gurbich Tatiana , Finn Robert D. , Iqbal Zamin , Roberts Leah W. , Lees John A. 
Published: April 2024

AbstractSummaryMetagenome Assembled Genomes (MAGs) are often incomplete, with sequences missing due to errors in assembly or low coverage. Incomplete MAGs present a particular challenge for identification of shared genes within a microbial population, known as core genes, as a core gene missing in only a few assemblies will result in it being mischaracterized at a lower frequency. Here, we present CELEBRIMBOR, a snakemake pangenome analysis pipeline which uses a measure of genome completeness to automatically adjust the frequency threshold at which core genes are identified, enabling accurate core gene identification in MAGs.Availability and implementationCELEBRIMBOR is published under open source Apache 2.0 licence athttps://github.com/bacpop/CELEBRIMBORand is available as a Docker container. Supplementary material is available in the online version of the article.
Reconstructing bat antiviral immunity using epithelial organoids
Authors: Kellner Max J. , Zelger Patrick , Monteil Vanessa , Pei Gang , Onji Masahiro , Nayak Komal , Zilbauer Matthias , Balkema-Buschmann Anne , Dorhoi Anca , Mirazimi Ali , Penninger Josef M. 
Published: April 2024

SummaryBats are remarkably resilient to viruses with pandemic potential. To resolve largely unknown molecular mechanisms governing their exceptional antiviral immunity, we established an organoid platform to model the entire respiratory airway and intestinal epithelium of the important viral reservoir speciesRousettus aegyptiacus(Egyptian fruit bat). These bat organoids exhibit an unexpected diversity of cell types and support replication of highly pathogenic zoonotic viruses including Marburg virus (MARV) and MERS-Coronavirus. Following virus infection, bat organoids unleash a strong interferon response, uniquely regulated through virus-dependent and virus-independent mechanisms. By contrast, MARV infected human organoids fail to induce an antiviral gene response and express pro-inflammatory cytokines after interferon stimulation, revealing important molecular differences between bats and humans with implications for lethal Marburg virus infections in primates. These data provide the most comprehensive organoid platform in bats to decode species-specific differences and uncover fundamental principles of bat disease resilience to emerging viruses with pandemic potential.
Strength of Activation and Temporal Dynamics of BioLuminescent-Optogenetics in Response to Systemic Injections of the Luciferin
Authors: Murphy Emily F. , Means Aniya , Li Chen , Baez Hector , Gomez-Ramirez Manuel 
Published: April 2024

AbstractBioLuminescent OptoGenetics (“BL-OG”) is a chemogenetic method that can evoke optogenetic reactions in the brain non-invasively. In BL-OG, an enzyme that catalyzes a light producing reaction (i.e., a luciferase) is tethered to an optogenetic element that is activated in response to bioluminescent light. Bioluminescence is generated by injecting a chemical substrate (luciferin, e.g., h-Coelenterazine; h-CTZ) that is catalyzed by the luciferase. By directly injecting the luciferin into the brain, we showed that bioluminescent light is proportional to spiking activity, and this relationship scales as a function of luciferin dosage. Here, we build on these previous observations by characterizing the temporal dynamics and dose response curves of BL-OG effects to intravenous (IV) injections of the luciferin. We imaged bioluminescence through a thinned skull of mice running on a wheel, while delivering h-CTZ via the tail vein with different dosage concentrations and injection rates. The data reveal a systematic relationship between strength of bioluminescence and h-CTZ dosage, with higher concentration generating stronger bioluminescence. We also found that bioluminescent activity occurs rapidly (< 60 seconds after IV injection) regardless of concentration dosage. However, as expected, the onset time of bioluminescence is delayed as the injection rate decreases. Notably, the strength and time decay of bioluminescence is invariant to the injection rate of h-CTZ. Taken together, these data show that BL-OG effects are highly consistent across injection parameters of h-CTZ, highlighting the reliability of BL-OG as a non-invasive neuromodulation method.
Deep Brain Stimulation of Nucleus Basalis of Meynert improves learning in rat model of dementia
Authors: Kumbhare Deepak , Rajagopal Megan , Toms Jamie , Freelin Anne , Weistroffer George , McComb Nicholas , Karnam Sindhu , Azghadi Adel , Murnane Kevin S. , Baron Mark S. , Holloway Kathryn L. 
Published: April 2024

ABSTRACTBackgroundDeep brain stimulation (DBS) of the nucleus basalis of Meynert (NBM) has been preliminarily investigated as a potential treatment for dementia. The degeneration of NBM cholinergic neurons is a pathological feature of many forms of dementia. Although stimulation of the NBM has been demonstrated to improve learning, the ideal parameters for NBM stimulation have not been elucidated. This study assesses the differential effects of varying stimulation patterns and duration on learning in a dementia rat model.Methods192-IgG-saporin (or vehicle) was injected into the NBM to produce dementia in rats. Next, all rats underwent unilateral implantation of a DBS electrode in the NBM. The experimental groups consisted of i-normal, ii-untreated demented, and iii-demented rats receiving NBM DBS. The stimulation paradigms included testing different modes (tonic and burst) and durations (1-hr, 5-hrs, and 24-hrs/day) over 10 daily sessions. Memory was assessed pre- and post-stimulation using two established learning paradigms: novel object recognition (NOR) and auditory operant chamber learning.ResultsBoth normal and stimulated rats demonstrated improved performance in NOR and auditory learning as compared to the unstimulated demented group. The burst stimulation groups performed better than the tonic stimulated group. Increasing the daily stimulation duration to 24-hr did not further improve cognitive performance in an auditory recognition task and degraded the results on a NOR task as compared with 5-hr.ConclusionThe present findings suggest that naturalistic NBM burst DBS may offer a potential effective therapy for treating dementia and suggests potential strategies for the reevaluation of current human NBM stimulation paradigms.
Inhibition of PAK2 in endothelial cells suppresses tumor angiogenesis and promotes immune sensitization through CXCL10
Authors: Corriveau Jeanne , Monot Pascale , Delisle Chantal , Goyette Marie-Anne , Gasse Camille , El Bakkouri Yassine , Hoang Trang , Côté Jean-François , Gratton Jean-Philippe 
Published: April 2024

ABSTRACTTumor angiogenesis is induced by the secretion of pro-angiogenic factors and results in a disorganized tumor vasculature. The p21-activated kinase 2 (PAK2) is involved in endothelial cell (EC) migration, an essential step of angiogenesis. However, the involvement of PAK2 in tumor angiogenesis remains ill-defined. Here, we show that, during orthotopic tumor growth, the specific deletion of PAK2 in ECs reduces tumor size and angiogenesis. In addition, endothelial-specific loss of PAK2 was found to normalize the remaining tumor blood vessels, favoring innate immune cells infiltration. Importantly, we uncovered a role for PAK2 in regulating chemokine expression, notably CXCL10. Secretion of CXCL10 from ECs is enhanced following PAK2 depletion and its expression is essential for the inhibitory effects of PAK2 silencing on EC spouting. Furthermore, neutralization of CXCL10 in mice reversed the effects induced by the deletion of PAK2 on tumor vasculature and immune composition of tumors. Together, our findings identify endothelial PAK2 as a potential target to reduce tumor angiogenesis and reprogram ECs to promote immune cell infiltration within tumors through the expression of CXCL10.