bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptomic analysis of an in vitro murine model of ovarian carcinoma: Functional similarity to the human disease and identification of prospective tumoral markers and targets
Authors: Urzúa Ulises , Roby Katherine F. , Gangi Lisa M. , Cherry James M. , Powell John I. , Munroe David J. 
Published: October 2005

AbstractOvarian cancer is an aggressive disease of poor prognostic when detected at advanced stage. It is widely accepted that the ovarian surface epithelium plays a central role in disease etiology, but little is known about disease progression at the molecular level. To identify genes involved in ovarian tumorigenesis, we carried out a genome‐wide transcriptomic analysis of six spontaneously transformed mouse ovarian surface epithelial (MOSE) cell lines, an in vitro model for human ovarian carcinoma. Loess normalization followed by statistical analysis with control of multiple testing resulted in 509 differentially expressed genes using an adjusted P‐value ≤0.05 as cut‐off. The top 20 differentially expressed genes included 10 genes (Spp1, Cyp1b1, Btg1, Cfh, Mt1, Mt2, Igfbp5, Gstm1, Gstm2, and Esr1) implicated in various aspects of ovarian carcinomas, and other 3 genes (Gsto1, Lcn7, and Alcam) associated to breast cancer. Upon functional analysis, the majority of alterations affected genes involved in glutathione metabolism and MAPK signaling pathways. Interestingly, over 20% of the aberrantly expressed genes were related to extracellular components, suggestive of potential markers of disease progression. In addition, we identified the genes Pura, Cnn3, Arpc1b, Map4k4, Tgfb1i4, and Crsp2 correlated to in vivo tumorigenic parameters previously reported for these cells. Taken together, our findings support the utility of MOSE cells in studying ovarian cancer biology and as a source of novel diagnostic and therapeutic targets. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Involvement of protein phosphatase 2A in the maintenance of E‐cadherin‐mediated cell–cell adhesion through recruitment of IQGAP1
Authors: Takahashi Kazuhide , Nakajima Eri , Suzuki Katsuo 
Published: October 2005

AbstractSerine/threonine protein phosphatase (PP) 2A regulates many biological processes, however it remains unclear whether PP2A participates in cadherin‐mediated cell–cell adhesion. We show here that the core enzyme of PP2A (PP2A‐AC) is localized in the cell–cell adhesion sites between adjacent cells and associated with the E‐cadherin‐catenins complex in non‐malignant human mammary epithelial (HME) cells at confluence. Treatment of the cells with either okadaic acid (OA), an inhibitor of PP2A, or siRNA for the regulatory subunit A of PP2A (PP2A‐A) caused disruption of cell–cell adhesion and F‐actin assembly, without affecting the complex formation of E‐cadherin with β‐ and α‐catenins. While a small GTPase Rac and its effector IQGAP1 were associated with the E‐cadherin‐catenins complex, either OA or PP2A‐A siRNA concomitantly induced the dissociation of IQGAP1, but not Rac, from the complex and the internalization of E‐cadherin from the cell surface. We therefore propose that PP2A plays a crucial role in the maintenance of cell–cell adhesion through recruitment of IQGAP1 to the Rac‐bound E‐cadherin‐catenins complex. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Implication of the hypoxia response element of the vegf promoter in mouse models of retinal and choroidal neovascularization, but not retinal vascular development
Authors: Vinores Stanley A. , Xiao Wei‐Hong , Aslam Sadia , Shen Jikui , Oshima Yuji , Nambu Hiroyuki , Liu Hansheng , Carmeliet Peter , Campochiaro Peter A. 
Published: October 2005

AbstractRetinal neovascularization (NV) and macular edema, resulting from blood‐retinal barrier (BRB) breakdown, are major causes of visual loss in ischemic retinopathies. Choroidal NV (CNV) occurs in diseases of the retinal pigmented epithelium/Bruch's membrane complex and is another extremely prevalent cause of visual loss. We used mice in which the hypoxia response element (HRE) is deleted from the vascular endothelial growth factor (vegf) promoter (Vegfδ/δ mice) to explore the role of induction of VEGF through the HRE in these disease processes. Compared to wild type (Vegf+/+) mice with oxygen‐induced ischemic retinopathy (OIR) in which vegf mRNA levels were increased and prominent retinal NV and BRB breakdown occurred, Vegfδ/δ littermates with OIR failed to increase vegf mRNA levels in the retina and had significantly less retinal NV and BRB breakdown, but showed prominent dilation of some superficial retinal vessels. Vegf+/δ littermates with ischemic retinopathy developed comparable retinal NV to Vegf+/+ mice, exhibited intermediate levels of BRB breakdown, and did not show vasodilation. In a mouse model of CNV, due to laser‐induced rupture of Bruch's membrane, the area of CNV at Bruch's membrane rupture sites was more than tenfold greater in Vegf+/+ mice than in Vegfδ/δ littermates. In contrast to these dramatic differences in pathologic ocular NV, Vegfδ/δ mice showed subtle differences in retinal vascular development compared to Vegf+/+ mice; it was slightly delayed, but otherwise normal. These data suggest that induction of VEGF through the HRE in its promoter is critical for retinal and CNV, but not for retinal vascular development. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Platelet derived growth factor induced tenascin‐C transcription is phosphoinositide 3‐kinase/Akt‐dependent and mediated by Ets family transcription factors
Authors: Jinnin Masatoshi , Ihn Hironobu , Asano Yoshihide , Yamane Kenichi , Trojanowska Maria , Tamaki Kunihiko 
Published: October 2005

AbstractPrevious studies have identified several cytokines as inducers of tenascin‐C (TN‐C) expression in various tissue culture systems. However, the signaling pathways of the regulation of TN‐C expression are almost unknown. In this study, we clarified the molecular mechanism(s) underlying the regulation of the TN‐C gene by platelet derived growth factor (PDGF) in cultured human dermal fibroblasts. PDGF induced the expression of TN‐C protein as well as mRNA in a dose‐dependent manner. Actinomycin D, an RNA synthesis inhibitor, significantly blocked the PDGF‐mediated upregulation of TN‐C mRNA expression, whereas cycloheximide, a protein synthesis inhibitor, did not. The PDGF‐mediated induction of TN‐C expression was inhibited by the treatment of fibroblasts with a selective phosphoinositide 3‐kinase (PI3K) inhibitor, wortmannin, or LY294002. These results suggest that PDGF induced the expression of TN‐C at a transcriptional level via phosphoinositide3‐kinase/Akt signaling pathways. We performed serial 5′ deletions and a transient transfection analysis to define the region in the TN‐C promoter mediating the responsiveness to PDGF. Overexpression of Sp1, Ets1, or Ets2 activated the TN‐C promoter and superinduced TN‐C promoter activity stimulated by PDGF, whereas overexpression of Fli1 inhibited the effects of PDGF on TN‐C expression. Mutation of the Sp1/3 binding sites or Ets binding sites in the TN‐C promoter region responsible to PDGF abrogated the PDGF‐inducible promoter activity. Immunoprecipitation analysis revealed that Sp1, Ets1, and Ets2 form a transcriptionally active complex. On the other hand, the interaction of Fli1 with Sp1 decreased after PDGF treatment. These results suggest that the upregulation of TN‐C expression by PDGF involves Ets family transcription factors, co‐operating with Sp1. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
The Feeder layer‐mediated extended lifetime of cultured human skin keratinocytes is associated with altered levels of the transcription factors Sp1 and Sp3
Authors: Masson‐Gadais Bénédicte , Fugère Claudia , Paquet Claudie , Leclerc Steeve , Lefort Natalie R. , Germain Lucie , Guérin Sylvain L. 
Published: October 2005

AbstractPrimary cultured epithelial cells that are used for basic research are often cultivated on plastic whereas those used for clinical purposes are usually cultured in the presence of a feeder layer. Here, we examined the influence of a feeder layer on the expression, affinity and DNA binding ability of the transcription factors, Sp1 and Sp3 in primary cultures of human skin keratinocytes. Co‐culturing both newborn and adult skin keratinocytes with lethally irradiated 3T3 cells as a feeder layer contributed to maintain the cell's morphological and growth characteristics and delayed terminal differentiation in vitro. 3T3 also stabilized the DNA binding properties of Sp1 without altering its transcription. Stimulation of Sp1/Sp3 expression appears to be mediated through cell–cell interactions and by factors secreted by 3T3. Thus, we propose that the feeder layer delay terminal differentiation of primary cultured skin keratinocytes by preventing extinction of transcription factors, like Sp1 and Sp3, which play pivotal functions in the cell cycle. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
CRH functions as a growth factor/cytokine in the skin
Authors: Slominski A. , Zbytek B. , Pisarchik A. , Slominski R.M. , Zmijewski M.A. , Wortsman J. 
Published: October 2005

AbstractWe tested the effect of CRH and related peptides in a large panel of human skin cells for growth factor/cytokine activities. In skin cells CRH action is mediated by CRH‐R1, a subject to posttranslational modification with expression of alternatively spliced isoforms. Activation of CRH‐R1 induced generation of both cAMP and IP3 in the majority of epidermal and dermal cells (except for normal keratinocytes and one melanoma line), indicating cell type‐dependent coupling to signal transduction pathways. Phenotypic effects on cell proliferation were however dependent on both cell type and nutrition conditions. Specifically, CRH stimulated dermal fibroblasts proliferation, by increasing transition from G1/0 to the S phase, while in keratinocytes CRH inhibited cell proliferation. In normal and immortalized melanocytes CRH effect showed dichotomy and thus, it inhibited melanocyte proliferation in serum‐containing medium CRH through G2arrest, while serum free media led instead to CRH enhanced DNA synthesis (through increased transition from G1/G0to S phase and decreased subG1signal, indicating DNA degradation). CRH also induced inhibition of early and late apoptosis in the same cells, demonstrated by analysis with the annexin V stains. Thus, CRH acts on epidermal melanocytes as a survival factor under the stress of starvation (anti‐apoptotic) as well as inhibitor of growth factors induced cell proliferation. In conclusion, CRH and related peptides can couple CRH‐R1 to any of diverse signal transduction pathways; they also regulate cell viability and proliferation in cell type and growth condition‐dependent manners. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Basolateral aromatic amino acid transporter TAT1 (Slc16a10) functions as an efflux pathway
Authors: Ramadan Tamara , Camargo Simone M.R. , Summa Vanessa , Hunziker Peter , Chesnov Serge , Pos Klaas M. , Verrey Francois 
Published: October 2005

AbstractBasolateral efflux is a necessary step in transepithelial (re)absorption of amino acids from small intestine and kidney proximal tubule. The best characterized basolateral amino acid transporters are y+LAT1‐4F2hc and LAT2‐4F2hc that function as obligatory exchangers and thus, do not contribute to net amino acid (re)absorption. The aromatic amino acid transporter TAT1 was shown previously to localize basolaterally in rat's small intestine and to mediate the efflux of L‐Trp in the absence of exchange substrate, upon expression in Xenopus oocytes. We compared here the amino acid influx and efflux via mouse TAT1 in Xenopus oocytes. The results show that mTAT1 functions as facilitated diffusion pathway for aromatic amino acids and that its properties are symmetrical in terms of selectivity and apparent affinity. We show by real‐time RT‐PCR that its mRNA is highly expressed in mouse small intestine mucosa, kidney, liver, and skeletal muscle as well as present in all other tested tissues. We show that mTAT1 is not N‐glycosylated and that it localizes to the mouse kidney proximal tubule. This expression is characterized by an axial gradient similar to that of the luminal neutral amino acid transporter B0AT1 and shows the same basolateral localization as 4F2hc. mTAT1 also localizes to the basolateral membrane of small intestine enterocytes and to the sinusoidal side of perivenous hepatocytes. In summary, we show that TAT1 is a basolateral epithelial transporter and that it can function as a net efflux pathway for aromatic amino acids. We propose that it, thereby, may supply parallel exchangers with recycling uptake substrates that could drive the efflux of other amino acids. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Transforming growth factor β1 regulates follistatin mrna expression during in vitro bovine granulosa cell differentiation
Authors: Fazzini Monica , Vallejo Griselda , Colman‐Lerner Alejandro , Trigo Romina , Campo Stella , Barañao J. Lino S. , Saragüeta Patricia E. 
Published: October 2005

AbstractIn order to test the hypothesis that transforming growth factor β (TGF‐β) acts by FS regulation on bovine granulosa cells in in vitro differentiation, we analyzed the effect of TGF‐β1 on follistatin mRNA expression in three differentiation states of bovine granulosa cells. We showed a positive regulation of FS mRNA after TGF‐β1 (1 ng/ml) treatment of freshly isolated granulosa cells from small‐medium antral follicles (2–8 mm). This effect was abolished by the addition of exogenous follistatin (100 ng/ml), suggesting that this effect could be mediated by activin. Although these cells showed a similar effect on FS mRNA expression after treatment with activin‐A, a soluble form of activin receptor type IIA was unable to inactivate the TGF‐β effect. When we tested the TGF‐β effect on FS mRNA in different granulosa cell states, TGF‐β1 regulation was associated with progesterone production only in freshly isolated cells. The amount of total activin‐A produced by first passage cells (dedifferentiated cells), was ten times smaller than the one measured in a conditioned medium from freshly isolated cells (mature cells). The TGF‐β1‐dependent FS mRNA expression persisted in first passage cells without changes with FS addition. On the other hand, the BGC‐1 granulosa cell line (immature cells) produced large amounts of activin‐A regulated by TGF‐β1 and an invariable steady state of FS mRNAs. In summary, our results showed that FS mRNA expression is regulated by TGF‐β1 independently of activin effects in differentiated granulosa cells. J. Cell. Physiol. 207: 40–48, 2006. © 2005 Wiley‐Liss, Inc.
ErbB‐receptors expression and survival in breast carcinoma: A 15‐year follow‐up study
Authors: Bianchi Simonetta , Palli Domenico , Falchetti Mario , Saieva Calogero , Masala Giovanna , Mancini Biancamaria , Lupi Ramona , Noviello Cristiana , Omerovic Jasminka , Paglierani Milena , Vezzosi Vania , Alimandi Maurizio , Mariani‐Costantini Renato , Ottini Laura 
Published: October 2005

AbstractAberrant expression of the epidermal growth factor receptor family has been implicated in the pathogenesis and progression of breast cancer and associated with poor prognosis. To evaluate the prognostic impact of the ErbB receptors expression profile, we analyzed a well‐characterized series of 145 primary breast carcinomas for the simultaneous expression of epidermal growth factor receptor (EGFR/HER‐1), ErbB‐2 (HER‐2), ErbB‐3 (HER‐3), and ErbB‐4 (HER‐4), using immunohistochemistry. Tumors were considered negative or positive for each marker when less than or more than 25% of the cancer cells were immunopositive. Expression of EGFR, ErbB‐2, ErbB‐3, and ErbB‐4 was observed in 31 (21.4%), 65 (44.8%), 72 (49.7%), and 81 (55.9%) of the cases, respectively. There were significant associations between EGFR expression and pT status (P = 0.01), and between ErbB‐3 expression and pN (P = 0.003), menopausal (P = 0.01) and PR (P < 0.001) status. The majority of the cases co‐expressed two or more receptors. ErbB‐3 resulted positive in 51/81 (63.0%) of the ErbB‐4 positive cases and ErbB‐3/ErbB‐4 co‐expression was statistically significant (P = 0.0003). As expected, ErbB‐2 expression was associated with reduced overall survival at 15 years of follow‐up (P = 0.04), even after adjusting for a series of other prognostic factors (P = 0.05). Moreover, cumulative analysis of ErbB‐2/3/4 expression showed a strong positive association between higher total ErbB‐2/3/4 expression score and worse prognosis (P = 0.002). The simultaneous expression in cancer cells of more than one ErbB receptor identifies a subset of breast cancer patients at high risk for poor survival. J.Cell.Physiol. © 2005 Wiley‐Liss, Inc.
Graph relations, clique divergence and surface triangulations
Authors: Larrión F. , Neumann‐Lara V. , Pizaña M.A. 
Published: October 2005

AbstractThis work has two aims: first, we introduce a powerful technique for proving clique divergence when the graph satisfies a certain symmetry condition. Second, we prove that each closed surface admits a clique divergent triangulation. By definition, a graph is clique divergent if the orders of its iterated clique graphs tend to infinity, and the clique graph of a graph is the intersection graph of its maximal complete subgraphs. © 2005 Wiley Periodicals, Inc. J Graph Theory
Biocomposites of Plasticized Starch Reinforced with Cellulose Crystallites from Cottonseed Linter
Authors: Lu Yongshang , Weng Lihui , Cao Xiaodong 
Published: October 2005

AbstractSummary: Environmentally friendly starch biocomposites were successfully developed using a colloidal suspension of cottonseed linter cellulose crystallite as a filler to reinforce glycerol plasticized starch (PS). The cellulose crystallites, having lengths of 350 ± 70 nm and diameters of 40 ± 8 nm on average, were prepared from cottonseed linters by acid hydrolysis. The dependence of morphology and properties of the PS‐based biocomposites on cellulose crystallites content in the range from 0 to 30 wt.‐% was investigated by scanning electron microscopy, differential scanning thermal analysis, dynamic mechanical thermal analysis, and measurements of mechanical properties and water absorption. The results indicate that the strong interactions between fillers and between the filler and PS matrix play a key role in reinforcing the resulting composites. The PS/cellulose crystallite composites, conditioned at 50% relative humidity, undergo an increase in both tensile strength and Young's modulus from 2.5 MPa for PS film to 7.8 MPa and from 36 MPa for PS film to 301 MPa. Further, incorporating cottonseed linter cellulose crystallites into PS matrix leads to an improvement in water resistance for the resulting biocomposites.The mechanical behaviors of the starch‐based biocomposites as a function of cellulose crystallites content.magnified imageThe mechanical behaviors of the starch‐based biocomposites as a function of cellulose crystallites content.
Ternary Complex Formation in Aqueous Solution between a β‐Cyclodextrin Polymer, a Cationic Surfactant and DNA
Authors: Galant Céline , Amiel Catherine , Auvray Loïc 
Published: October 2005

AbstractSummary: Polyelectrolyte complexes have been elaborated by mixing in water neutral poly(β‐CD), a cationic surfactant (DTAC) and herring sperm DNA fragments. The driving forces for the poly(β‐CD)/DTAC/DNA association in aqueous solution are, on the one hand, reversible inclusion interactions between the CD cavities of poly(β‐CD) and the alkyl group of DTAC, leading to the formation of a polycation and, on the other hand, electrostatic interactions between the opposite charges of the cationic surfactant and anionic DNA. Viscometry and SANS have been used to prove the occurrence of such ternary complexes in dilute aqueous solutions.
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Preparation and Interfacial Properties of a Novel Biodegradable Polymer Surfactant: Poly(ethylene oxide monooleate‐block‐DL‐lactide)
Authors: Nishino Satoru , Kitamura Yoshiro , Kishida Akio , Yoshizawa Hidekazu 
Published: October 2005

AbstractSummary: In this paper, we report a novel synthesis of poly(ethylene oxide monooleate‐block‐DL‐lactide) (MOPEO‐PLA) in the presence of stannous 2‐ethylhexanoate catalyst. By utilizing the surfactant property and the reactive double bond of the amphiphilic MOPEO‐PLA, various characteristics of PLA microspheres, such as surface and internal structure, surface morphology, release property, and so on, may potentially be controlled. MOPEO‐PLA was found to be hydrophobic enough to prevent loss by dissolution into aqueous solution, which is often a problem for MOPEO. Furthermore, the interfacial tension measurements of a MOPEO‐PLA/toluene/water system revealed that MOPEO‐PLA had a good surface activity almost equal to that of MOPEO. The MOPEO‐PLA/PLA blend films were prepared by solvent casting on a water layer. Contact‐angle measurements of MOPEO‐PLA/PLA blend films confirmed that the hydrophilic PEO segments were selectivity accumulated at the oil/water interface. Moreover, the surface free energy on the ‘water side’ of the MOPEO‐PLA/PLA blend films was increased because of the increase in polar components as a result of the ether bonds of the PEO segments.Schematic illustration of the adsorption property of a) MOPEO‐PLA with a high‐molecular‐weight PLA segment and b) MOPEO‐PLA with a low‐molecular‐weight PLA segment at an ethyl acetate/water interface.magnified imageSchematic illustration of the adsorption property of a) MOPEO‐PLA with a high‐molecular‐weight PLA segment and b) MOPEO‐PLA with a low‐molecular‐weight PLA segment at an ethyl acetate/water interface.
UV‐Photocrosslinking of Inulin Derivatives to Produce Hydrogels for Drug Delivery Application
Authors: Tripodo Giuseppe , Pitarresi Giovanna , Palumbo Fabio Salvatore , Craparo Emanuela Fabiola , Giammona Gaetano 
Published: October 2005

AbstractSummary: In this work, INU, a natural polysaccharide, has been chemically modified in order to obtain new photocrosslinkable derivatives. To reach this goal, INU has been derivatized with MA thus obtaining four samples (INU‐MA derivatives) as a function of the temperature and time of reaction. An aqueous solution of the derivative INU‐MA1 was irradiated by using a UV lamp with an emission range from 250 to 364 nm and without using photoinitiators. The obtained hydrogel showed a remarkable water affinity but it underwent a partial degradation in simulated gastric fluid. To overcome this drawback, INU‐MA1 was derivatized with SA thus obtaining the INU‐MA1‐SA derivative designed to produce a hydrogel showing a low swelling and an increased chemical stability in acidic medium. Ibuprofen, as a model drug, was loaded by soaking into INU‐MA1 and INU‐MA1‐SA hydrogels and its release from these matrices was evaluated in simulated gastrointestinal fluids. INU‐MA1 hydrogel showed the ability to quickly release the entrapped drug thus indicating its potential as a matrix for an oral formulation. INU‐MA1‐SA hydrogel showed a pH‐responsive drug delivery. Therefore it is a promising candidate for controlled drug release in the intestinal tract.
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Synthesis and Rheological Properties of Polylactide/Poly(ethylene glycol) Multiblock Copolymers
Authors: Li Feng , Li Suming , Vert Michel 
Published: October 2005

AbstractSummary: Ring‐opening polymerization of D,L‐lactide was carried out in the presence of poly(ethylene glycol), using Zn powder as catalyst. The hydroxyl‐capped PLA‐PEG‐PLA triblock copolymers were coupled with adipoyl chloride at different molar ratios under mild conditions. N‐Dimethylaminopyridine (DMAP) was used as catalyst of the coupling reaction. The resulting PLA/PEG multiblock copolymers were characterized by various analytical techniques such as IR, 1H NMR, SEC, and DSC. Sol‐gel transition properties of the multiblock copolymers were investigated by mechanical rheology. The data showed that the sol‐gel transition temperature and the transition modulus increased with increasing molecular weight and the solution concentration of the multiblock copolymers.Variation of storage modulus (G′) and loss modulus (G″) as a function of temperature for a 20% sample of MB3.magnified imageVariation of storage modulus (G′) and loss modulus (G″) as a function of temperature for a 20% sample of MB3.