bluuebunny/crossref_metadata_2025_split huggingface dataset:
LOX-1 and MMP-9 Inhibition Attenuates the Detrimental Effects of Delayed rt-PA Therapy and Improves Outcomes After Acute Ischemic Stroke
Authors: Arkelius Kajsa , Wendt Trevor S. , Andersson Henrik , Arnou Anaële , Gottschalk Michael , Gonzales Rayna J. , Ansar Saema 
Published: March 2024

BACKGROUND:
Acute ischemic stroke triggers endothelial activation that disrupts vascular integrity and increases hemorrhagic transformation leading to worsened stroke outcomes. rt-PA (recombinant tissue-type plasminogen activator) is an effective treatment; however, its use is limited due to a restricted time window and hemorrhagic transformation risk, which in part may involve activation of MMPs (matrix metalloproteinases) mediated through LOX-1 (lectin-like oxLDL [oxidized low-density lipoprotein] receptor 1). This study’s overall aim was to evaluate the therapeutic potential of novel MMP-9 (matrix metalloproteinase 9) ± LOX-1 inhibitors in combination with rt-PA to improve stroke outcomes.

METHODS:
A rat thromboembolic stroke model was utilized to investigate the impact of rt-PA delivered 4 hours poststroke onset as well as selective MMP-9 (JNJ0966) ±LOX-1 (BI-0115) inhibitors given before rt-PA administration. Infarct size, perfusion, and hemorrhagic transformation were evaluated by 9.4-T magnetic resonance imaging, vascular and parenchymal MMP-9 activity via zymography, and neurological function was assessed using sensorimotor function testing. Human brain microvascular endothelial cells were exposed to hypoxia plus glucose deprivation/reperfusion (hypoxia plus glucose deprivation 3 hours/R 24 hours) and treated with ±tPA and ±MMP-9 ±LOX-1 inhibitors. Barrier function was assessed via transendothelial electrical resistance, MMP-9 activity was determined with zymography, and LOX-1 and barrier gene expression/levels were measured using qRT-PCR (quantitative reverse transcription PCR) and Western blot.

RESULTS:
Stroke and subsequent rt-PA treatment increased edema, hemorrhage, MMP-9 activity, LOX-1 expression, and worsened neurological outcomes. LOX-1 inhibition improved neurological function, reduced edema, and improved endothelial barrier integrity. Elevated MMP-9 activity correlated with increased edema, infarct volume, and decreased neurological function. MMP-9 inhibition reduced MMP-9 activity and LOX-1 expression. In human brain microvascular endothelial cells, LOX-1/MMP-9 inhibition differentially attenuated MMP-9 levels, inflammation, and activation following hypoxia plus glucose deprivation/R.

CONCLUSIONS:
Our findings indicate that LOX-1 inhibition and ± MMP-9 inhibition attenuate negative aspects of ischemic stroke with rt-PA therapy, thus resulting in improved neurological function. While no synergistic effect was observed with simultaneous LOX-1 and MMP-9 inhibition, a distinct interaction is evident.
Beyond the Theosophical Paradigm: Ilme kṣnum and the Entangled History of Modern Parsis
Authors: Errichiello Mariano 
Published: March 2024

Abstract
In the early twentieth century, an esoteric interpretation of Zoroastrianism known as Ilme kṣnum became popular among the Parsis of India. Although research on the subject is scant, most scholars suggest that Ilme kṣnum draws largely upon the ideas promoted by the Theosophical Society in India. By examining primary sources in Gujarati, the present article illustrates the interpretation of the Zoroastrian cosmology proposed by Ilme kṣnum. Through a comparative analysis of its main concepts and terms, Ilme kṣnum is historicized in the context of the relations of the Parsi community with the Persianate and Western worlds. By framing Ilme kṣnum as a reconciliation between Persianate and Western forms of knowledge, the present article looks at historical entanglements as resources for the Parsi quest for religious authenticity, placing Zoroastrianism in global religious history.
A bio-economic model for estimating economic values of important production traits in the black soldier fly (Hermetia illucens)
Authors: Zaalberg R.M. , Nielsen H.M. , Noer N.K. , Schou T.M. , Jensen K. , Thormose S. , Kargo M. , Slagboom M. 
Published: March 2024

Abstract
Black soldier fly (BSF) larvae are exceptionally efficient at converting otherwise unusable waste products, while having a minimal impact on the environment. To optimise the production of BSF larvae, selective breeding could be used. With selective breeding, the best individuals to breed the next generation are selected, for example, with a breeding goal. This study aimed to make the first step towards defining such a breeding goal, by estimating the economic values (EV) of traits that are important for the production of BSF larvae. EV were estimated for larval mass on day 15, feed intake, feed conversion ratio, growth rate, dry mass-, protein- and fat content, development time, eggs per fly, egg hatching rate, and larval mortality. A bio-economic model was used to estimate the profit of a BSF production system that produces 1000 kg of wet larvae at harvesting. The EV of a trait was estimated as the increase in profit when the trait value was increased by 10%, whilst the other traits remained unchanged. EV were expressed per 10% increase, and changes in revenues and costs were specified. The results showed traits with the highest EV were related to composition (dry mass-, and protein-content) and growth (larval mass, development time, growth rate). Traits related to reproduction and flies (development from pupa to fly, number of eggs) had the lowest EV. Due to the lack of information on genetic parameters, the EV are expressed per +10% of the mean value of a trait. In the future, when more information is available, the EV should be expressed per genetic standard deviation. In conclusion, this is the first study that estimated EV for an insect species. The results are a first step towards developing a more sophisticated breeding program for optimizing BSF production.
Observed Climatology and Variability of Cattle Heat Stress in Australia
Authors: Cowan Tim , Wheeler Matthew C. , Cobon David H. , Gaughan John B. , Marshall Andrew G. , Sharples Wendy , McCulloch Jillian , Jarvis Chelsea 
Published: March 2024

Abstract
Exposure to weather extremes, such as heatwaves, can cause discomfort, harm, or death in grazing cattle in pastures. While the Australian Bureau of Meteorology issues sheep graziers alerts when there is an exposure risk to chill for livestock, there is no equivalent alert for heat stress for Australian cattle. Before any such alert system can be developed, a robust assessment and comparison of relevant cattle thermal stress indices is required. This study evaluates and compares the multiyear climatology of three cattle thermal heat stress indices across Australia in the warm season months (October–March). The same indices are then used to assess historical Australian heat events where cattle died from heat exposure. These events are based off official records and survey responses from northern Australian graziers. In the seven historical heat events studied, high relative humidity combined with low wind speeds, or high solar exposure combined with high surface temperatures, exacerbated the impact of heat stress on cattle. In the two historic events where multiple compounding weather factors combined (e.g., high humidity, low winds, and high solar exposure), the cattle mortality levels were significantly high. These events were characterized by rainy conditions followed by a rapid warming, meaning cattle were likely unable to acclimatize to such dramatic temperature changes. This study highlights the need for using more than one thermal stress index when verifying cattle heat stress events and, importantly, calls for further research on standardizing the risk classifications of these thermal indices for cattle in Australia’s variable climate.

Significance Statement
Cattle across Australia’s northern tropical and semiarid regions often experience extreme hot and humid conditions in the summer months, which increases the risk of heat stress. This is the first study of its kind to evaluate observations of cattle heat stress across Australia using indices that describe the combined effects of solar exposure, wind speed, relative humidity, and surface temperatures. These cattle heat stress indices can be used to evaluate historical cattle mortality events in feedlots and in grazed pastures. This study lays the groundwork for the development of Australian-wide cattle heat stress forecast products on the 7-day to multiweek time scales.
Nurses Caring for Older Adult Family Members: Disclosing Caregiving to Work Supervisors
Authors: Xu Jiayun , Peng Yisheng , Foli Karen J. 
Published: March 2024

Background: Disclosure of family caregiving to work supervisors is needed for nurses to access work support for family caregiving. Little is known about characteristics of nurses who decide to/not to disclose family caregiving to supervisors. Objective: The objective was to examine characteristics of nurses based on whether they disclosed caregiving responsibilities to their nursing supervisors and describe reasons for non-disclosure. Methods: This was a secondary analysis of a cross-sectional survey. Registered nurses who had a work supervisor and cared for an older adult family member completed a survey including demographics, work and caregiving characteristics, and disclosure. Descriptive statistics and binary logistic regression were conducted. Results: The sample included 162 nurses. Participants were on average 50 years old, 90.1% female, 65.4% married, and 80.9% were caring for a parent or parent-in-law. The disclosure was more likely among nurses who provided higher intensity care (hours of care), cared for a parent or in-law, or had a quality caregiver-care recipient relationship. Reasons for non-disclosure included wanting to separate personal and work life, discomfort, and fear of consequences. Conclusions: Nurses struggle with similar disclosure challenges as other family caregivers. Care intensity, caregiver-care recipient relationships, and care stress were associated with disclosure behaviors.
What is the Role of Motives for Smartphone Use in Elementary School Students? Problematic Smartphone Use, Family Satisfaction, Loneliness, and Academic Performance
Authors: Przepiorka Aneta , Blachnio Agata , Kot Paweł , Cudo Andrzej 
Published: March 2024

The cross-sectional study aimed to examine the relationships between motives for smartphone use, family satisfaction, loneliness, grade point average (GPA), and problematic smartphone use (PSU). It included 519 elementary school students aged 12–16 years ( M = 13.87, SD = 1.01). The authors used the Smartphone Addiction Scale, the Smartphone Usage Motivation Scale, the Illinois Loneliness and Social Dissatisfaction Questionnaire, and the Satisfaction With Family Life Scale. For girls, the study revealed a significant indirect relationship between loneliness and GPA via social interactions. There was also a significant indirect relationship between loneliness and PSU via relaxing entertainment. For boys, there were significant indirect relationships between loneliness and PSU via escapism and between age and PSU via loneliness. The results may serve as a basis for therapeutic interventions and prevention programs against PSU.
Comparison between prefabricated ankle–foot orthoses, Dyna Ankle and UD Flex, in patients with hemiplegia
Authors: Lee Su Ji , Kim Tae Yong , Kim Kyung Min , Cho Sung-Rae 
Published: March 2024

Objective To compare the kinematic effects of two widely-used prefabricated ankle–foot orthoses (AFOs), the Dyna Ankle (DA) and UD Flex (UD), on the gait cycle of patients with hemiplegia due to cerebral palsy or acquired brain injury. Methods This was a retrospective cohort study involving 29 patients. Gait analysis results were assessed under three conditions: barefoot, with the DA, and with the UD. Friedman tests and post hoc analysis with Bonferroni correction were performed to assess differences between the three conditions. Results The DA significantly improved ankle dorsiflexion during the mid-swing phase, making it more effective in correcting foot drop compared with the UD (DA: 2.28°, UD: 0.44°). Conversely, the UD was more effective in preventing knee flexion during the loading response (DA: 28.11°, UD: 26.72°). Conclusions The DA improved ankle dorsiflexion during the swing phase significantly more than that with the UD in patients with hemiplegia. Compared with the DA, the UD more effectively prevented increased knee flexion during the loading response. The choice to prescribe these orthoses should consider individual patient characteristics.
Efficacy and safety of galcanezumab as chronic cluster headache preventive treatment under real world conditions: Observational prospective study
Authors: Lamas Pérez Raquel , Millán-Vázquez Manuel , González-Oria Carmen 
Published: March 2024

Background Calcitonin gene-related peptide has shown to play a central role in cluster headache (CH) pathophysiology. A clinical trial with galcanezumab was carried out in chronic cluster headache (CCH) but did not meet its primay endpoint. However, its off-label use in patients with CCH refractory to other therapies could be considered. We aimed to asses the efficacy and safety of galcanezumab as CCH preventive treatment in a real-life setting. Methods An observational study was conducted. Patients with CCH who received at least one dose of 240 mg of galcanezumab. Results Twenty-one patients who tried a mean of 6.3 ± 1.9 preventive therapies, including onabotulinumtoxinA in 90.5%. At baseline, the median of frequency was 60 (37.5–105) monthly attacks with 10 (8.3–10) points in pain intensity (Numerical Rating Scale). After one month, the frequency decreased to 31 (10.5–45) ( p = 0.003) with 8.5 (8–9.5) intensity ( p = 0.007); 10 (47.6%) patients were 50% responders of whom four (19%) were 75% responders. Of the 15 patients with 3 months of follow-up, seven (46.6%) reduced their frequency by 50% and four (26.6%) by 75%, with 40 (10–60) monthly attacks ( p = 0.07) and pain intensity of 8 (5–10) ( p = 0.026). Some 52% patients experienced adverse events, mostly mild. Conclusions In our cohort of refractory CCH, galcanezumab was effective in almost 50% of patients. This finding supports individual off-label treatment attempts.
Quality assurance in surgical trials of arteriovenous grafts for haemodialysis: A systematic review, a narrative exploration and expert recommendations
Authors: Kingsmore David B , Edgar Ben , Aitken Emma , Calder Francis , Franchin Marco , Geddes Colin , Inston Nick , Jackson Andrew , Jones Rob G , Karydis Nikolaos , Kasthuri Ram , Mestres Gaspar , Papadakis Georgios , Sivaprakasam Rajesh , Stephens Mike , Stevenson Karen , Stove Callum , Szabo Lazslo , Thomson Peter C , Tozzi Matteo , White Richard D 
Published: March 2024

Background: Introducing new procedures and challenging established paradigms requires well-designed randomised controlled trials (RCT). However, RCT in surgery present unique challenges with much of treatment tailored to the individual patient circumstances, refined by experience and limited by organisational factors. There has been considerable debate over the outcomes of arteriovenous grafts (AVG) compared to AVF, but any differences may reflect differing practice and potential variability. It is essential, therefore, when considering an RCT of a novel surgical procedure or device that quality assurance (QA) is defined for both the new approach and the comparator. The aim of this systematic review was to evaluate the QA standards performed in RCT of AVG using a multi-national, multi-disciplinary approach and propose an approach for future RCT. Method: The methods of this have been previously registered (PROSPERO: CRD420234284280) and published. In summary, a four-stage review was performed: identification of RCT of AVG, initial review, multidisciplinary appraisal of QA methods and reconciliation. QA measures were sought in four areas – generic, credentialing, standardisation and monitoring, with data abstracted by a multi-national, multi-speciality review body. Results: QA in RCT involving AVG in all four domains is highly variable, often sub-optimally described and has not improved over the past three decades. Few RCT established or defined a pre-RCT level of experience, none documented a pre-trial education programme, or had minimal standards of peri-operative management, no study had a defined pre-trial monitoring programme, and none assessed technical performance. Conclusion: QA in RCT is a relatively new area that is expanding to ensure evidence is reliable and reproducible. This review demonstrates that QA has not previously been detailed, but can be measured in surgical RCT of vascular access, and that a four-domain approach can easily be implemented into future RCT.
Pain Management in Surgical Treatment of Facial Fractures: Alternative Approaches to Opioid Use
Authors: Tucker Jacqueline , Oxford Madison , Goldenberg Dana , Ziai Kasra , Lighthall Jessyka G. 
Published: March 2024

Study Design Literature review. Objective This review aims to explore current opioid use trends for surgical management of facial fractures, as well as methods and alternative treatments to decrease opioid use. Methods Review of selected literature from Medline and Google Scholar. Results Despite the devastating effects of the opioid epidemic and recent policy changes aimed at reducing unnecessary opioid prescription, opioids remain commonly used for pain management after facial fracture repair. Recently, use of multimodal analgesic therapy has been suggested to decrease opioid dosage utilized for post-operative pain control. Alternatives to medication therapies have been proposed for pain management; however, standardized recommendations for pain management in facial fracture patients remain unclear. Conclusions Further research is required to establish evidence-based recommendations for pain management in craniofacial fracture repair.
The BMP7-Derived Peptide p[63-82] Reduces Cartilage Degeneration in the Rat ACLT–pMMx Model for Posttraumatic Osteoarthritis
Authors: Ripmeester Ellen G.J. , Steijns Jessica S.J.J. , Wijnands Karolina A.P. , Stassen Roderick H.M.J. , Pitelka Vasek , Peeters Laura C.W. , Cremers Andy , Astryde Nzekui M. S. A. , Chabronova Alzbeta , Surtel Don A.M. , Emans Pieter J. , van den Akker Guus G.H. , van Rietbergen Bert , van Rhijn Lodewijk W. , Caron Marjolein M.J. , Welting Tim J.M. 
Published: March 2024

Objective Osteoarthritis (OA) is characterized by articular cartilage erosion, pathological subchondral bone changes, and signs of synovial inflammation and pain. We previously identified p[63-82], a bone morphogenetic protein 7 (BMP7)-derived bioactive peptide that attenuates structural cartilage degeneration in the rat medial meniscal tear-model for posttraumatic OA. This study aimed to evaluate the cartilage erosion-attenuating activity of p[63-82] in a different preclinical model for OA (anterior cruciate ligament transection—partial medial meniscectomy [anterior cruciate ligament transection (ACLT)-pMMx]). The disease-modifying action of the p[63-82] was followed-up in this model for 5 and 10 weeks. Design Skeletally mature male Lewis rats underwent ACLT-pMMx surgery. Rats received weekly intra-articular injections with either saline or 500 ng p[63-82]. Five and 10 weeks postsurgery, rats were sacrificed, and subchondral bone characteristics were determined using microcomputed tomography (µCT). Histopathological evaluation of cartilage degradation and Osteoarthritis Research Society International (OARSI)-scoring was performed following Safranin-O/Fast Green staining. Pain-related behavior was measured by incapacitance testing and footprint analysis. Results Histopathological evaluation at 5 and 10 weeks postsurgery showed reduced cartilage degeneration and a significantly reduced OARSI score, whereas no significant changes in subchondral bone characteristics were found in the p[63-82]-treated rats compared to the saline-treated rats. ACLT-pMMx-induced imbalance of static weightbearing capacity in the p[63-82] group was significantly improved compared to the saline-treated rats at weeks 5 postsurgery. Footprint analysis scores in the p[63-82]-treated rats demonstrated improvement at week 10 postsurgery. Conclusions Weekly intra-articular injections of p[63-82] in the rat ACLT-pMMx posttraumatic OA model resulted in reduced degenerative cartilage changes and induced functional improvement in static weightbearing capacity during follow-up.
Prediction of 3-Dimensional Coverage Surface Area of the Femoral Head in Hip Dysplasia Through Conventional Computed Tomography
Authors: Kamenaga Tomoyuki , Ritacco Lucas , Slullitel Pablo A. , Nahal Chadi , Nepple Jeffrey J. , Clohisy John C. , Pascual-Garrido Cecilia 
Published: March 2024

Background: Assessment of 3-dimensional (3D) femoral head coverage is critical in evaluating, preoperative planning, and treating hip dysplasia. Purpose: To (1) propose a mathematical model to establish 3D femoral head coverage using conventional computed tomography (CT), (2) determine the correlation of 2D parameters with 3D coverage, and (3) characterize the patterns of dysplasia based on 3D morphology. Study Design: Cross-sectional study; Level of evidence, 3. Methods: We identified 30 patients (n = hips) with symptomatic dysplasia and 30 patients (n = hips) without dysplasia. Patients with dysplastic hips were matched with regard to sex, age, and body mass index to those with nondysplastic hips. Preoperative CTs were analyzed using 3D software, and 3D femoral head surface area coverage (FHSAC; in %) was assessed in 4 quadrant zones: anteromedial, anterolateral, posteromedial, and posterolateral. To assess lateral coverage of the femoral head, we introduced the anterolateral femoral head coverage angle (ALFC) and the posterolateral femoral head coverage angle (PLFC). Results: Reduced femoral head coverage was more pronounced in dysplastic versus nondysplastic hips in the anterolateral quadrant (18% vs 40.7%, respectively) and posterolateral quadrant (35.8% vs 56.9%, respectively) ( P < .0001 for both). Dysplastic hips had smaller ALFC and PLFC (18.4° vs 38.7°; P < .0001; 47.2° vs 72.3°; P = .0002). Anterolateral and posterolateral FHSAC were strongly correlated with the ALFC ( r = 0.88; P < .0001) and the PLFC ( r = 0.82; P < .0001) along with the lateral center-edge angle (anterolateral, r = 0.75; P < .0001; posterolateral, r = 0.73; P < .0001). Prediction models established for FHSAC had strong agreement with explanatory CT variables (anterolateral: r = 0.91; P < .0001; posterolateral: r = 0.90; P < .0001). The cutoff values for anterolateral and posterolateral FHSAC were 25% and 41%, respectively. In dysplastic hips, global deficiency was most common (15/30 hips), 9 hips showed an anterolateral deficiency, and 4 hips had a posterolateral deficiency pattern. Conclusion: The ALFC and The PLFC were strongly correlated with 3D lateral FHSAC and were able to predict 3D coverage accurately.