bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro Antimicrobial Activity of Cucurbita Pepo on the Selected Bacterial Pathogen and Ethnobotanical Knowledge of the Community at Gara Muleta, Girawa, Eastern Ethiopia
Authors: Wolde Wondimagegn , Nasir Mohammed , Ataro Zerihun , Seyoum Ayichew 
Published: March 2024

Abstract
Background
In developing countries, 80% of the population use traditional medicine to meet their primary healthcare needs. Cucurbita pepo is a widely used food and medicinal plant as a natural anti-inflammatory, antiviral, and analgesic agent for treating various public health problems. This study was conducted to evaluate the in vitro antimicrobial activity of Cucurbita Pepo on the selected bacterial pathogen and Ethnobotanical knowledge Methods: The study was conducted at Gara Muleta, Eastern Ethiopia from April 30 to May 30, 2020. Sixty experimental tests were performed to evaluate the in vitro antimicrobial activity of Cucurbita pepo seed extracts using three extraction methods (water-based, methanol-based, and chloroform) at different concentrations (higher, medium, and lower concentrations). An experimental study was carried out using three standard pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) using the disc diffusion and broth dilution techniques. A community-based cross-sectional study was carried out among 384 households in Girawa town to assess the ethnobotanical knowledge of the community regarding Cucurbita pepo.
Results
Water, methanol, and chloroform extracts of Cucurbita pepo seeds showed maximum inhibition zones of 9.0 mm, 12.0 mm, and 10.0 mm and minimum inhibitory concentrations of 18, 24, and 20 mg/mL, respectively, against P. aeruginosa. Of the 384 study participants, 376 (98.2%) of the participants had ethnobotanical knowledge of Cucurbita pepo seeds. However, 240 (62.5%) and 255 (66.4%) of the study participants had no information about the medical use and health benefits of Cucurbita pepo seeds, respectively.
Conclusion
The methanol and chloroform extracts of Cucurbita pepo seeds possessed notable antibacterial activity, but water extract showed no zone of inhibition against E. coli and S. aureus.
Simultaneous Estimation and Stability Indicating Method of Vildagliptin and Dapagliflozin by Rp-hplc in Pharmaceutical Dosage Form
Authors: Patel Bhavesh H. , Teraiya Nishit H. 
Published: March 2024

Abstract

Vildagliptin and Dapagliflozin drugs are used for the management of diabetes.The primary objective of the proposed research was to develop and validate analytical methods for simultaneous quantification of vildagliptin and dapagliflozin in pharmaceutical dosage form. Both the drugs were subjected to force degradation to ensure stability of pharmaceutical products.The HPLC method development was carried out on C18 column (250mm*4.6mm,5µm particle size). The chromatographic separation was achieved by isocratic mode with a mixture of Methanol: 0.01% Trifluoro acetic acid (pH-2.78) in the ratio of 95:05 % v/v as mobile phase. Flow rate was kept at 0.8 ml/min and selected wavelength for estimation was 210 nm. Stress testing with acid, alkaline, oxidative and thermal degradation was performed.
The retention time was found to be 2.282 min and 4.070 min for vildagliptin and dapagliflozin, respectively. The drugs response found to be linear with correlation coefficient value was nearly 0.999 for vildagliptin and dapagliflozin with linearity range of 10 – 60 µg/ml. The recoveries from studies were found to be 99.78% and 100.17% for vildagliptin and dapagliflozin respectively. LOD and LOQ were found to be lower, hence the method proved sensitivity. Method was validated as per ICH guideline Q2(R1).
Drugs had shown more degradation in oxidative condition, 10.62% and 13.52% for vildagliptin and dapagliflozin, respectively. The proposed method would be successfully applied for the stability indicating, impurity separation, qualitative and quantitative estimation of vildagliptin and dapagliflozin in pharmaceutical dosage form and in process quality control analysis.
Donor MHC-specific Thymus Vaccination for Immunocompatible Allotransplantation
Authors: Liu Yang , Feng Hexi , Li Ke , Li Ruiyi , Zhang Xiao-Jie , Tian Ye , Fang Yujiang , Zhou Yanjie , Liu Ling , Zhang Xiaoqing 
Published: March 2024

Abstract

Organ transplantation is the last-resort option to treat organ failure. However, less than 10% of patients benefit from this only option due to lack of major histocompatibility complex (MHC)-matched donor organs and 25-80% of donated organs could not find MHC-matched recipients. T cell allorecognition is the principal mechanism for allogeneic graft rejection. We herein present a “donor MHC-specific thymus vaccination” (DMTV) strategy to induce T cell tolerance to both autologous and allogeneic donor MHC. Allogeneic MHC molecules were expressed in the recipient thymus through adeno-associated virus infection, which led to stable expression of allogeneic MHC together with the autologous MHC in the engineered thymus. During local T cell education, those T cells recognizing either autologous MHC or allogeneic MHC were equally depleted. We constructed C57BL/6-MHC and BALB/c-MHC dual immunocompatible mice via thymus vaccination of C57BL/6-MHC into the BALB/c thymus and observed long-term tolerance after transplantation of C57BL/6 skin and C57BL/6 mouse embryonic stem cells into the vaccinated BALB/c mice. We also validated our DMTV strategy in a bone marrow, liver, thymus (BLT)-humanized mouse model for immunocompatible allotransplantation of human embryonic stem cells. Our study suggests that DMTV is a potent avenue to introduce a donor compatible immune system in recipients, which overcomes the clinical dilemma over the extreme shortage of MHC-matched donor organs for treating patients with end-stage organ failure.
FAKTOR-FAKTOR YANG MELATARBELAKANGI LESBIAN DAN KONDISI PSIKOLOGISNYA
Authors: . Nurkholis 
Published: March 2024

Penelitian ini merupakan penelitian deskriptif kualitatif, dengan 1 subjek . Metode pengumpulan data menggunakan wawancara. Analisa data yang digunakan adalah analisa data kualitatif, kemudian uji keabsahan datanya menggunakan teknik triangulasi yaitu triangulasi sumber yaitu teman dekat subjek. Hasil penelitian menunjukan bahwa : (1) Faktor internal meliputi : (a) Persepsi subjek, dimana subjek berfikir tidak akan hamil jika berpacaran dengan sesama perempuan, (b) Dorongan-dorongan atau kecendrungan penyuka sesama jenis yang sudah ada dalam diri subjek, (c) Adanya kontrol diri yang lemah, dimana subjek selalu terpengaruh keinginan-keinginan (ego) nya sendiri. (2) Faktor eksternal yang meliputi : (a) Adanya proses modeling dari perempuan yang berperilaku dan berpenampilan maskulin, (b) Adanya pengalaman buruk yang dialami subjek, yaitu ejekan dari teman-teman subjek sewaktu SMA, (c) Sikap ayah yang terkesan membiarkan perilaku subjek, walaupun sebenarnya ayah subjek tahu tentang perilakunya tersebut (Reinforcement positif), (d) Adanya pengalaman yang kurang menyenangkan terhadap lawan jenis (e) Adanya dukungan dari lingkungan sosial (Reinforcement positif), yaitu subjek pernah ikut dalam suatu oeganisasi atau komunitas lesbian. Kata kunci: Faktor-faktor psikologis, Lesbian. This research is qualitatif descriptive study, with 1 subjects who has sexual deviation “lesbian”. Data collection methods used were interviews. Analysis of the data used is the analysis of qualitatif data, and then test the validity of the data using triangulation techniques, namely the source of a close friend of the subject.The results showed that the factors underlying a lesbian that internal factors which include (1) The perception of the subject : (a) The subject is thinking will not get pregnant if she is dating other women, (b) Impulses or tendencies sex enthusiasts are already present in thesubject, (c) The existence of a weak self –control, which the subject is always affected by her own desires. (2) External factors which include : (a) The process of modeling the behavior of women and masculine look, (b) The existence of bad experiences suffered by the subject is in the form of ridicule from her friends during senior high school, (c) Her father attitude that seemed to let the behavior of subjects despite the fact that the subject’s father know about lesbian behavior (positive reinforcement), (d) The existence of her an unpleasant experience with the opposite sex, (e) The support of the social environment (positive reinforcement) that is the subject of ever joining a community organization or a lesbian. Keywords: Psychological factors, Lesbian.
Se correr o bicho pega, se ficar o bicho come!
Authors: De Paula Simone 
Published: March 2024

Este trabalho pretende apresentar a relação do sujeito no espaço erótico-relacional em tempos de relacionamentos atravessados pela tecnologia e pela robótica. O campo da sedução, fundamental para o despertar do desejo e da vivência de fantasias eróticas, modulado pela angústia, ainda funcionaria na relação com as máquinas? O filme Ich bin dein meinsch (Eu sou seu humano / Eu sou sua pessoa), traduzido por I’m your man, e no Brasil, O homem ideal, da diretora alemã Maria Schrader, me serve para algumas reflexões acerca desse tema.
Pengaruh Pengelolaan Keuangan Daerah dan Sistem Akuntansi Keuangan Daerah terhadap Kinerja Keuangan Pemerintah: Studi Empiris pada OPD Provinsi Sumatera Barat
Authors: Syafdan Tasya Aulia , Arza Fefri Indra 
Published: March 2024

This study aims to analyse the influence of regional financial management and regional financial accounting system on government financial performance. The research was conducted at the West Sumatera Province Regional Apparatus Organization. The sampling technique uses purposive sampling. Method Data collection was carried out using a questionnaire. Regression analysis Multiple tests were carried out to test the hypothesis using SPSS 20 with results research shows that regional financial management has a positive effect on government financial performance while the regional financial accounting system does not has an influence on the government's financial performance.
Pengaruh Alokasi Dana Desa (ADD) terhadap Tingkat Kemiskinan Masyarakat Desa Melalui Pertumbuhan Ekonomi Sebagai Variabel Intervening
Authors: Fatimah Ica Maulidna , Deviani Deviani 
Published: March 2024

This study aims to examine the effect of village fund allocation on poverty levels through economic growth. This research is an empirical study of districts/cities in the ten poorest provinces in Indonesia in 2021. This type of research is causal quantitative. The type of data used is secondary data. The secondary data used is data and information on village fund allocations, poverty rates and economic growth through the official BPS website and the village ministry. Methods of data analysis using Path Analysis (Path Analysis). The results of the research on the variable allocation of village funds with a probability value of 0.157 > 0.05, the allocation of village funds has a positive and insignificant effect on the poverty level of rural communities. The village fund allocation variable has a probability value of 0.375 > 0.05, so the village fund allocation has no positive and significant effect on economic growth. The economic growth variable with a probability value of 0.000 <0.05, means that economic growth has a negative and significant effect on the poverty level of rural communities. Hypothesis testing obtained a calculated t value = 1.1769 < t table 1.65 with a significance level of 0.05, so it can be concluded that the mediation coefficient is 0.030 and there is no intervening influence. The results obtained show that economic growth as an intervening variable has a positive and insignificant effect on the poverty level of rural communities.
Dampak Gadget Terhadap Perkembangan Sosial Anak Usia Sekolah Dasar
Authors: Haeroni Ranita , Eliasa Eva Imania 
Published: March 2024

Perkembangan teknologi yang makin pesat saat ini, sangat mempengaruhi seluruh lini kehidupan. Salah satu dampak perkembangan tersebut adalah maraknya penggunaan gadget. Gadget dimanfaatkan oleh seluruh kalangan, dari anak-anak hingga dewasa. Penggunaan gadget terbesar melanda siswa Sekolah Dasar. Gadget juga mempengaruhi perkembangan sosial anak. Artikel ini bertujuan untuk mengkaji dan menganalisis dampak penggunaan gadget terhadap perkembangan sosial anak Sekolah Dasar. Penelitian ini dilakukan dengan metode kualitatif melalui pendekatan deskriptif analitik. Teori dan dasar pembahasannya diambil dari hasil studi pustaka seperti buku, artikel, dan jurnal. Hasil penelitian menunjukkan bahwa sebagian besar anak menggunakan gadget untuk bermain game online, bermain sosial media, dan menonton film animasi. Gadget semestinya hanya digunakan sebagai media pembelajaran. Perhatian dari orang tua sangat dibutuhkan oleh anak untuk penggunaan gadget seuai fungsinya. Anak harus lebih diarahkan untuk melakukan kegiatan yang memiliki aktifitas fisik di lingkungannya agar anak dapat dengan mudah untuk bersosialisasi.Keywords: Gadget, Perkembangan Sosial Anak Sekolah Dasar
œCaring Snake & Ladder Learning Media Development For 2nd Year Students™ Competence In Reading Aloud At Mekarsari 03 Elementary School
Authors: Jannah Putri Asnes Miftahul , Neolaka Grace Amialia Anfetonanda 
Published: March 2024

This study aims to describe the results of the development of the "CARING" snake and ladder media for the ability to read loudly of second-grade students at Mekarsari 03 Elementary School. The method in this research is research and development (R&D) with the ADDIE model, which refers to five stages: analysis, design, development, implementation (implementations), and evaluation. The results of the study showed that the CARING snake and ladder media received a score of 72% from media experts, 78% from material experts, 95% from students, and 100% from teachers. Based on these results, the media was valid and very feasible for use to help grade 2 students' reading aloud skills at Mekarsari 03 Elementary School. Keywords: Snake & Ladder, learning media, reading aloud, elementary school
Clippers are superior to scissors in the collection of hair for chemical analysis in companion dogs: a Dog Aging Project preliminary study
Authors: Ditzler Bobbie , Evans Jeremy B. , Illing Kate , Kaeberlein Matt , Promislow Daniel E. L. , Brindle Eleanor , Hoffman Jessica M. , Creevy Kate E. 
Published: March 2024

Abstract

OBJECTIVE
To identify the safest, most efficient method for hair sample collection from companion dogs among clippers, scissors, and razors and to validate obtained samples with cortisol concentration analysis.

ANIMALS
25 healthy, privately owned dogs.

METHODS
2 hair samples were collected from each dog’s ischiatic region with different implements (scissors, razors, or clippers). The collecting clinician completed a Hair Collection Questionnaire (HCQ) for each sample that compared subjective sample quality, time of collection, restraint needed, and patient experience. Each sample was evaluated by cortisol enzyme immunoassay.

RESULTS
Clippers had higher overall HCQ scores than scissors, and scissors had higher HCQ scores than razors. Collection was faster for clippers than scissors, and scissors were faster than razors. There were no differences in sample quality between scissors and clippers, and sample quality was lower with razors. There was no difference in restraint needed or patient experience. Collection of long hair had higher HCQ scores than collection of medium and short hair. Collection of hair from dogs with an undercoat had higher HCQ scores than collection of hair from dogs without an undercoat. Dog size had no effect on HCQ score. Hair cortisol concentration did not vary between scissors or clippers (P = .111). Hair color and age did not affect hair cortisol concentration (P = .966 and P = .676, respectively).

CLINICAL RELEVANCE
Clippers are recommended for hair sample collection from companion dogs. Scissors are an adequate alternative.
Harmonizing Technology and Tradition: The Impact of Blended Teaching in Chinese Primary and Secondary Music Education
Authors: Zhi Guan Xing , Thoe Ng Khar 
Published: March 2024

Purpose：The purpose is to examine the effectiveness of blended teaching methods in music education, aiming to enhance student engagement, comprehension, and academic performance while addressing the challenges and opportunities of incorporating technology into traditional music pedagogy.

Theoretical References：This paper employs a theoretical framework that merges social constructivism and sociocultural theory with blended music education. It focuses on integrating technology to enhance personalized learning, critical thinking community building in music teaching, informed by Guan et al. (2023).

Methodology：This study utilized a quantitative research design with a novel 5-point Likert scale questionnaire involving 70 music educators from primary and secondary schools in central China. Data were collected through an online survey platform and analyzed in four dimensions using SPSS24, with methods including descriptive statistics, reliability and validity tests, and correlation analysis.

Results and Conclusions：The findings suggest that blended teaching significantly enhances the quality of music education, increases student engagement, and promotes diverse pedagogical approaches. Challenges include maintaining student engagement and navigating technological complexities, but overall, the benefits outweigh these hurdles.

Research Implications：This study highlights the significant impact of blended learning approaches on music education in Chinese elementary and middle schools. It calls for a systematic integration of these methods into music curricula to improve student learning experiences. Key recommendations include enhancing teachers' technological skills, ensuring students have equal access to digital resources, and utilizing technology to augment traditional teaching practices.

Originality/Value：This research stands out for its detailed exploration of blended teaching methods in Chinese music education, a topic not widely covered in existing studies. It presents evidence of both the advantages and challenges of using technology in music education, offering important insights and practical suggestions for educators, curriculum designers, and policymakers to better incorporate technology into music education strategies.
Kekuatan Tarik Terhadap Beberapa Sudut Serat Pada Polimer Matrik Komposit Yang Berpenguat Serat Bambu Dengan Perlakuan Di Alkali Untuk Pengembangan Bodi Dan Rangka Kendaraan Elektrik
Authors: Purwanto Hengki , Andary Siti Rodiyah , Hartono Hartono 
Published: March 2024

Composite Matrix Polymer is a material formed by two or more combinations, Composite Matrix Polymer is a material consisting of two basic materials, namely matrix and fiber. For fibers it has elastic properties, and the best tensile strength, but also cannot be used against high temperatures, while matric itself has tenacious, soft properties.And it is also binding if it is dry or has reached freezing. This research is to determine the material with natural fibers, namely bamboo fibers that have been processed to such an extent as to realize or entrepreneurial opportunities in the field of body and electric vehicle frames. For this reason, we made this research.To determine the effect of variations in the fibrous angles of composite matrix polymers with angles (0°), (-45°, 45°), and (-45°, 0°, 45°), With bamboo fiber reinforcement for the addition of bamboo fiber variations and with Alkaline treatment at the ratio of NaOH solution levels of 5%, 10%, and 15%, thus to carry out the addition of alkaline treatmentThis study uses experimental methods, and can determine the influence of angular variations, composite matrix polymer fibers with fiber angles (0°), (-45°, 45°), and (-45°, 0°, 45°) reinforced bamboo fibers, with the influence of variations in alkaline dosing of bamboo fibers and the ratio of NaOH solution levels of 5%, 10%, and 15% on the tensile strength of the material.The result of the tests that we have done, is the use of a good 5% alkaline content, a 5% alkaline solution content gets the best fiber speciment form than a 10% and 15% alkaline solution.
And the lowest result is an alkaline content of NaOH 15%. While the best result of angle variation is the angle variation (-45°, 0°, 45°) and the lowest is the angle variation (0°). Which will then be used as a method for learning or practicum modules at the Jember State Polytechnic.
Efektivitas Media Edamame Agar Dengan Penambahan Polymyxin B Sebagai Media Selektif Alternatif Bakteri Bacillus Subtilis
Authors: Nurhayati Dian , Andayani Nanik Andayani , Saing Muhammad Djabir Saing 
Published: March 2024

Media selektif Edamame Agar dengan penambahan Polymyxin B dibuat bertahap yaitu pembuatan tepung edamame dan pembuatan edamame agar + Polymyxin B. Penelitian dilaksanakan pada bulan Juni – September 2023, bertempat di Laboratorium Analisis Pangan Politeknik Negeri Jember. Penelitian bertujuan untuk mengetahui pertumbuhan bakteri Bacillus Subtillis pada edamame agar dengan penambahan Polymyxin B dan membuat SOP. Metode penelitian dengan membandingkan pertumbuhan bakteri Bacillus Subtilis pada edamame agar dengan media Mannitol Egg yolk polymyxin Agar .Hasil penelitian menunjukkan bahwa jumlah koloni bakteri Bacillus subtillis pada media dengan penambahan Polymyxin B 0,6 % , 1,1 %, 1,6 % dan media Mannitol Egg yolk polymyxin Agar dengan masa inkubasi 48 jam adalah 3,9 x 105, 2,2 x 105, 1,0 x 105 cfu/ml dan pada Mannitol Egg yolk polymyxin Agar 3,5 x 105 cfu/ml . Pengujian kimia terhadap koloni yang tumbuh pada keempat media menunjukkan hasil sama yaitu Katalase positip, Fermentasi Laktosa negatip , Fermentasi Glukosa positif dan Voges Proskauer positip. Pengecatan gram menunjukkan bakteri Bacilus subtilis yang tumbuh pada media Edamame agar dan Mannitol Egg yolk polymyxin Agar diperoleh hasil bentuknya batang dan berwarna biru yaitu termasuk bakteri gram positip.Kesimpulan penelitian adalah media edamame agar dengan penambahan Polymyxin B mampu menghambat bakteri gram negatip dan menumbuhkan bakteri gram positip Bacillus subtillis secara optimal . Bakteri Bacillus subtillis yang tumbuh terbanyak pada media edamame agar dengan penambahan Polymyxin B 0,6% dengan TPC 3,9 x 105 cfu/ml lebih tinggi dibandingkan dengan Mannitol Egg yolk polymyxin Agar dengan TPC 3,5 x 105 cfu/ml.
Analisis Keluaran Berkas Radiasi Sinar-X pada Pesawat Linear Accelerator (LINAC) Berdasarkan TRS 398 IAEA di RSUD Arifin Achmad Provinsi Riau
Authors: Cahyaningtyas Saphira Indah , Anggraini Rista Mutia , Fendriani Yoza 
Published: March 2024

Telah dilakukan penelitian tentang analisis keluaran berkas radiasi sinar-X pada pesawat Linear Accelerator (Linac). Analisis ini bertujuan untuk mengetahui keluaran berkas radiasi pada pesawat Linac menunjukkan nilai 1 cGy atau sama dengan 1 MU, yang diperoleh dari analisis ionisasi chamber, suhu, tekanan, efek polaritas, dan rekombinasi ion. Penelitian ini dilakukan menggunakan energi foton sebesar 6 MV dan energi elektron dengan variasi (4, 6, 8, 10, 12, dan 15) MeV. Luas lapangan yang digunakan yaitu 10 cm x 10 cm serta source to surface distance (SSD) 100 cm. Hasil analisis menunjukkan berkas energi foton 6 MV didapatkan nilai keluaran per 1 MU sebesar 1,0046 cGy/MU dengan deviasi 0,46%. Sedangkan pada berkas elektron energi 4 MeV sebesar 1,0112 cGy/MU dengan deviasi 1,12%, energi 6 MeV sebesar 1,0064 cGy/MU dengan deviasi 0,64%, energi 8 MeV sebesar 0,9991 cGy/MU dengan deviasi 0,09%, energi 10 MeV sebesar 0,9987 cGy/MU denganÂ deviasi 0,13%, energi 12 MeV sebesar 0,9978 dengan deviasi 0,22% dan energi 15 MeV sebesar 1,0013 cGy/MU dengan deviasi 0,13%. Dalam hal ini keluaran berkas radiasi sinar-X pada pesawat Linac (Linear Accelerator) di RSUD Arifin Achmad Provinsi Riau telah sesuai dengan standar TRS 398 IAEA dengan rentang deviasi pengukuran yaitu Â±3%.