bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and validation of machine learning models for predicting distant metastases in newly diagnosed colorectal cancer patients: A large‐scale and real‐world cohort study
Authors: Wei Ran , Yu Guanhua , Wang Xishan , Jiang Zheng , Guan Xu 
Published: March 2024

AbstractBackgroundMore accurate prediction of distant metastases (DM) in patients with colorectal cancer (CRC) would optimize individualized treatment and follow‐up strategies. Multiple prediction models based on machine learning have been developed to assess the likelihood of developing DM.MethodsClinicopathological features of patients with CRC were obtained from the National Cancer Center (NCC, China) and the Surveillance, Epidemiology, and End Results (SEER) database. The algorithms used to create the prediction models included random forest (RF), logistic regression, extreme gradient boosting, deep neural networks, and the K‐Nearest Neighbor machine. The prediction models' performances were evaluated using receiver operating characteristic (ROC) curves.ResultsIn total, 200,958 patients, 3241 from NCC and 197,717 CRC from SEER were identified, of whom 21,736 (10.8%) developed DM. The machine‐learning‐based prediction models for DM were constructed with 12 features remaining after iterative filtering. The RF model performed the best, with areas under the ROC curve of 0.843, 0.793, and 0.806, respectively, on the training, test, and external validation sets. For the risk stratification analysis, the patients were separated into high‐, middle‐, and low‐risk groups according to their risk scores. Patients in the high‐risk group had the highest incidence of DM and the worst prognosis. Surgery, chemotherapy, and radiotherapy could significantly improve the prognosis of the high‐risk and middle‐risk groups, whereas the low‐risk group only benefited from surgery and chemotherapy.ConclusionThe RF‐based model accurately predicted the likelihood of DM and identified patients with CRC in the high‐risk group, providing guidance for personalized clinical decision‐making.
Notch signaling genes and CD8+ T‐cell dynamics: Their contribution to immune‐checkpoint inhibitor therapy in oral squamous cell carcinoma: A retrospective study
Authors: Ogi Kazuhiro , Iwamoto Takahiro , Sasaya Takashi , Nishiyama Koyo , Tokura Takaaki , Sasaki Takanori , Dehari Hironari , Arihara Yohei , Murase Kazuyuki , Saito Masato , Someya Masanori , Takada Kohichi , Miyazaki Akihiro 
Published: March 2024

AbstractBackgroundAberrant Notch signaling pathway has been related with the tumorigenesis in head and neck region, involving oral cavity. Here, we report the correlation between mutations in the Notch signaling pathway and CD8+ T‐cell infiltration via PD‐L1, which lead to enhanced antitumor immunity and may target for immune‐checkpoint inhibitors (ICIs) therapy.MethodsThis retrospective study analyzed the results of immunohistochemical staining for PD‐L1 and CD8+ T‐cell infiltration in 10 patients and whole‐exome sequencing (WES) was conducted on five of these patients to identify frequently mutated genes.ResultsFour of 10 patients were positive for PD‐L1 and CD8+ T. By analyzing WES in three of these four patients, we notably identified the mutations of NOTCH1, FBXW7, and noncoding RNA intronic mutation in NOTCH2NLR in two of these three patients. This study may enable better selection of ICI therapy with CD8+ T‐cell infiltration via PD‐L1 expression for oral squamous cell carcinoma patients with mutations in Notch signaling pathway.
Ultrasound‐guided percutaneous high‐frequency irreversible electroporation in porcine livers using four electrode needles: A feasibility and safety study
Authors: Li Chang‐Tian , Zhao Guo‐Dong , Zou Wen‐Bo , Zhang Zhen‐Hua , Zhao Yi , Liu Rong 
Published: March 2024

AbstractBackgroundMalignant liver tumors seriously endanger human health. Among different therapeutic approaches, high‐frequency irreversible electroporation (H‐FIRE) is a recently emerging tumor ablation technique. The objective of this study was to evaluate the feasibility and safety of ultrasound‐guided percutaneous H‐FIRE using four electrode needles in porcine livers.MethodsTwelve experimental pigs underwent percutaneous H‐FIRE ablation using a compound steep‐pulse therapeutic device. Liver tissues adjacent to the gallbladder, blood vessels, and bile ducts were selected as the ablation targets. Pigs were randomly divided into three groups: (1) immediately after ablation (N = 4), (2) 2 days after ablation (N = 4), and (3) 7 days after ablation (N = 4). Blood routine, liver and kidney function, and myocardial enzyme levels were measured before and after ablation. Ultrasound, contrast‐enhanced ultrasound (CEUS), contrast‐enhanced magnetic resonance imaging (MRI), and hematoxylin–eosin staining were performed to evaluate the ablation performance.ResultsUltrasound‐guided percutaneous H‐FIRE ablations using four electrode needles were successfully performed in all 12 experimental pigs. The general conditions of the pigs, including postoperative activities and feeding behaviors, were normal, with no significant changes compared with the preoperative conditions. The imaging features of ultrasound, CEUS, and MRI demonstrated no significant changes in the gallbladder walls, bile ducts, or blood vessels close to the ablation areas. Laboratory tests showed that liver function indices and myocardial enzymes increased temporarily after H‐FIRE ablation, but decreased to normal levels at 7 days after ablation. Histopathological examinations of porcine liver specimens showed that this technique could effectively ablate the target areas without damaging the surrounding or internal vascular systems and gallbladder.ConclusionsThis study demonstrated the feasibility and safety of ultrasound‐guided percutaneous H‐FIRE ablation in porcine livers in vivo, and proposed a four‐needle method to optimize its clinical application.
Decreased interleukin‐17RA expression is associated with good prognosis in patients with colorectal cancer and inhibits tumor growth and vascularity in mice
Authors: Jiang Jeng‐Kai , Lin Chi‐Hung , Chang Ting‐An , Lo Liang‐Chuan , Lin Chien‐Ping , Lu Ruey‐Hwa , Yang Chih‐Yung 
Published: March 2024

AbstractBackgroundInterleukin‐17 (IL‐17) is a pro‐inflammatory cytokine that plays a vital role in the promotion of tumorigenesis in various cancers, including colorectal cancer (CRC). Based on current evidence, IL‐17 binds to interleukin‐17 receptor A (IL‐17RA); however, the role of IL‐17RA has not been elucidated in previous studies on CRC. In this study, we explored the role of IL‐17RA in human CRC tissues and the progression of CRC in humans and mice.MethodsThe expressions of IL‐17RA and epithelial‐mesenchymal transition (EMT)‐related genes were examined in CRC cells and tissue samples by quantitative real‐time polymerase chain reaction. The role of IL‐17RA in pathogenesis and prognosis was evaluated using a Chi‐squared test, Kaplan–Meier analysis, univariate, and multivariate Cox regression analysis in 133 CRC patients. A tumor‐bearing mice model was executed to evaluate the role of IL‐17RA in tumor growth, vascularity and population of infiltrating immune cells.ResultsIL‐17RA expression was found to be significantly higher in CRC tissues than in adjacent normal tissues. The expression of IL‐17RA in Stage IV patients was significantly higher than that in Stages I and II patients. Patients with high IL‐17RA expression exhibited significantly worse overall and CRC‐specific survival than those with low IL‐17RA expression. Functional assessment suggested that the knockdown of IL‐17RA expression distinctly suppressed cellular proliferation, migration, invasion, and EMT‐related gene expression. In a tumor‐bearing mouse model, decreased IL‐17RA expression significantly repressed tumor growth and vascularity and reduced the population of regulatory T cells (Tregs) and myeloid‐derived suppressor cells (MDSCs).ConclusionReduced IL‐17RA expression also suppressed cellular proliferation, migration, and invasion, and the expression of EMT genes. Knockdown of IL‐17RA inhibited tumor growth and vascularity and decreased the population of Tregs and MDSCs in mouse tumors. Overall, IL‐17RA expression was identified to be independently associated with the prognosis of patients with CRC.
Reduced‐dose chemotherapy followed by blinatumomab in induction therapy for newly diagnosed B‐cell acute lymphoblastic leukemia
Authors: Lu Jing , Zhou Huifen , Zhou Xin , Yang Yonggong , Tong Laigen , Miao Miao , Yang Xiaofei , Chen Suning 
Published: March 2024

AbstractBackgroundBlinatumomab early‐line treatment in B‐cell precursor acute lymphoblastic leukemia (B‐ALL) might improve clinical outcomes.MethodsWe conducted a retrospective real‐world cohort analysis in 20 newly diagnosed B‐ALL patients who received reduced‐dose chemotherapy (idarubicin, vindesine, and dexamethasone) for 1–3 weeks, followed by blinatumomab for 1–4 weeks as an induction therapy.ResultsAt the end of the induction therapy, a complete remission rate of 100% was achieved; 17 (85%) patients were minimal residual disease (MRD) negative (<1 × 10−4). Adverse events (AEs) were reported in 12 (60%) patients—43.8% were grade 1–2 and 56.2% were grade 3–4. No incidence of neurotoxicity or grade ≥3 cytokine release syndrome was reported.ConclusionsBlinatumomab demonstrated a significant improvement in clinical outcomes in patients with newly diagnosed B‐ALL irrespective of their poor‐risk factor status and the pretreatment blast burden.
A New Rare Halogenated Depside from Lichen and Study of its Anti‐Proliferative Activity
Authors: Bauri Ajoy K. , Dionicio Ines Castro , Arellano Eric Salinas , Jeyaraj Jonathan G. , Foro Sabine , Carcache de Blanco Esperanza J. 
Published: March 2024

AbstractLichens are a symbiotic association of algae and fungus, belonging to the family Parmeliaceae. Some lichen species are edible and used as an active ingredient for preparation of exotic spices as well as folklore medicine to cure different kinds of ailments. A specimen of lichen was collected from Munner in the Kerala State of South India for chemical profiling. Chemical analyses of the diethyl ether extract of the defatted lichen led to the isolation of six phenols 1–6 with variation of relative abundance. Amongst them, the relative abundance of compound 3 was the greatest (1 % of crude extract) and it was identified as atranorin. The structures of known compounds were confirmed by comparison of their 1H‐NMR, 13C NMR, and mass data with published values available in the literature. In vitro bioassay for anti‐proliferative activity of these compounds has been conducted against various human cancer cell lines in comparison with paclitaxel as control using SRB assay. Interestingly, a new compound 5 was found along with previously reported compounds from this lichen. This new compound was designated as fluoroatranorin 5 which was reported for the first time herein. The structural characterization of a new depside was determined by spectral methods such as 1H‐NMR, 13C NMR, 19F NMR, IR, LC‐HRESI‐MS, and LC–MS/MS study. Its structure was confirmed by single crystal X‐ray diffraction study. This new compound was designated as fluoroatranorin 5 which was reported first time herein. Anti‐proliferative activity of all these compounds was evaluated against six different cancer cell lines. The inhibitory activity, IC50 value of compounds 1–3 and 5 exhibited at 99.64, 102.04, 109.20, 53.0 and 2.4 μM on cancer cell lines HT‐29 (colon), Hela (cervical), HT‐29, HPAC (pancreas) and A2780 (ovarian cancer cell line) respectively in comparison with paclitaxel as control. The new compound 5 exhibited significant activity with IC50 value 2.4 μM on A2780 ovarian cancer cell line.
Dual role of mesenchymal stem/stromal cells and their cell‐free extracellular vesicles in colorectal cancer
Authors: Saadh Mohamed J. , Mohamed Asma'a H. , Almoyad Muhammad Ali Abdullah , Allela Omer Qutaiba B. , Amin Ali H. , Malquisto April Ann , Jin Wong Tze , Sârbu Ioan , AlShamsi Faisal , Elsaid Fahmy Gad , Akhavan‐Sigari Reza 
Published: March 2024

AbstractColorectal cancer (CRC) is one of the main causes of cancer‐related deaths. However, the surgical control of the CRC progression is difficult, and in most cases, the metastasis leads to cancer‐related mortality. Mesenchymal stem/stromal cells (MSCs) with potential translational applications in regenerative medicine have been widely researched for several years. MSCs could affect tumor development through secreting exosomes. The beneficial properties of stem cells are attributed to their cell–cell interactions as well as the secretion of paracrine factors in the tissue microenvironment. For several years, exosomes have been used as a cell‐free therapy to regulate the fate of tumor cells in a tumor microenvironment. This review discusses the recent advances and current understanding of assessing MSC‐derived exosomes for possible cell‐free therapy in CRC.
Betaine postpones hyperglycemia‐related senescence in ovarian and testicular cells: Involvement of RAGE and β‐galactosidase
Authors: Mohammadi Neda , Hemmati Mina , Motlagh Behrouz , Biyabani Arezou 
Published: March 2024

AbstractThe structural and functional disorders of the testis and ovary are one of the main complications of hyperglycemia. Betaine is a trimethyl glycine with antioxidant, antidiabetic, and anti‐inflammatory potential. The aim of this study is to investigate the potential of betaine on the expression of aging and oxidative stress markers in ovarian and testicular cells under hyperglycemic conditions. Testicular and ovarian cells were subjected to four different conditions, including normal glucose and hyperglycemia, with or without betaine (5 mM). The cells with hyperglycemia saw an increase in malondialdehyde (MDA), methylglyoxal (MGO), expression of a receptor for AGE, and aging‐related genes (β‐GAL), and a decrease in the activity of antioxidant enzymes including catalase, glutathione peroxidase, and superoxide dismutase. The treatment with betaine, in contrast, decreased the amount of MGO and MDA, and also downregulated aging‐related signaling. Although hyperglycemia induces senescence in testicular and ovarian cells, the use of betaine may have a protective effect against the cell senescence, which may be useful in the management of infertility.
Tissue engineering innovations to enhance osseointegration in immediate dental implant loading: A narrative review
Authors: Saleh Hasani Jebelli Mohammad , Yari Amir , Nikparto Nariman , Cheperli Sahar , Asadi Amirali , Darehdor Amirmohammad Arabi , Nezaminia Sayna , Dortaj Dorara , Hasani Mehraban Saeed , Hakim Lotfollah Kamali 
Published: March 2024

AbstractThe demand for efficient and accelerated osseointegration in dental implantology has led to the exploration of innovative tissue engineering strategies. Immediate implant loading reduces treatment duration and necessitates robust osseointegration to ensure long‐term implant success. This review article discusses the current studies of tissue engineering innovations for enhancing osseointegration in immediate dental implant loading in the recent decade. Keywords “tissue engineering,” “osseointegration,” “immediate implant loading,” and related terms were systematically searched. The review highlights the potential of bioactive materials and growth factor delivery systems in promoting osteogenic activity and accelerating bone regeneration. The in vivo experiment demonstrates significantly improved osseointegration in the experimental group compared to traditional immediate loading techniques, as evidenced by histological analyses and biomechanical assessments. It is possible to revolutionize the treatment outcomes and patient satisfaction in dental implants by integrating bioactive materials and growth factors.
Goat mammary epithelial cells provide a better expression system for production of recombinant human bone morphogenetic protein 2 compared to Chinese hamster ovarian cells
Authors: Ganesan Venkateswaran , Ulgekar Goutam , Ramalingam Anandhi , Sen Sharma Souvik , Ganguli Nirmalya , Majumdar Subeer S. 
Published: March 2024

AbstractBone Morphogenetic Protein 2 (BMP2), a member of the Transforming Growth Factor‐β (TGF‐β) super family of proteins and is instrumental in the repair of fractures. The synthesis of BMP2 involves extensive post‐translational processing and several studies have demonstrated the abysmally low production of rhBMP2 in eukaryotic systems, which may be due to the short half‐life of the bioactive protein. Consequently, production costs of rhBMP2 are quite high, limiting its availability to the general populace. Therefore, there is an urgent need to identify better in‐vitro systems for large scale production of rhBMP2. In the present study, we have carried out a comparative analysis of rhBMP2 production by the conventionally used Chinese Hamster ovarian cells (CHO) and goat mammary epithelial cells (GMEC), upon transfection with appropriate construct. Udder gland cells are highly secretory, and we reasoned that such cells may serve as a better in‐vitro model for large scale production of rhBMP2. Our results indicated that the synthesis and secretion of bioactive rhBMP2 by goat mammary epithelial cells was significantly higher as compared to that by CHO‐K1 cells. Our results provide strong evidence that GMECs may serve as a better alternative to other mammalian cells used for therapeutic protein production.
Dual‐Emissive Iridium(III) Complexes and Their Applications in Biological Sensing and Imaging
Authors: Ruan Zhipeng , Yang Jun , Li Yonghua , Zhang Kenneth Yin 
Published: March 2024

AbstractPhosphorescent iridium(III) complexes are widely recognized for their unique properties in the excited triplet state, making them crucial for various applications including biological sensing and imaging. Most of these complexes display single phosphorescence emission from the lowest‐lying triplet state after undergoing highly efficient intersystem crossing (ISC) and ultrafast internal conversion (IC) processes. However, in cases where these excited‐state processes are restricted, the less common phenomenon of dual emission has been observed. This dual emission phenomenon presents an opportunity for developing biological probes and imaging agents with multiple emission bands of different wavelengths. Compared to intensity‐based biosensing, where the existence and concentration of an analyte are indicated by the brightness of the probe, the emission profile response involves modifications in emission color. This enables quantification by utilizing the intensity ratio of different wavelengths, which is self‐calibrating and unaffected by the probe concentration and excitation laser power. Moreover, dual‐emissive probes have the potential to demonstrate distinct responses to multiple analytes at separate wavelengths, providing orthogonal detection capabilities. In this concept, we focus on iridium(III) complexes displaying fluorescence‐phosphorescence or phosphorescence‐phosphorescence dual emission, along with their applications as biological probes for sensing and imaging.
Novel application of the Diabolo technique to restrict pulmonary blood flow in a single ventricle patient with a right ventricle to pulmonary artery conduit
Authors: Stanley Helen M. , Callahan Ryan 
Published: March 2024

AbstractBalancing pulmonary and systemic circulations in single ventricle patients with a conduit after Stage 1 palliation is challenging. A transcatheter intervention for excessive pulmonary blood flow would provide benefit. We report a case of a critically ill single ventricle patient with symptoms of excessive pulmonary blood flow after Stage 1 despite maximal medical therapy. The patient underwent percutaneous intraluminal downsizing of the right ventricle to pulmonary artery conduit using a novel application of the Diabolo‐covered stent technique, with subsequent clinical improvement. A second catheterization was performed during the interstage period with successful dilation of the stent to achieve appropriate saturations. The Diabolo technique can be successfully employed in this population to restrict pulmonary blood flow and has the advantage of being adjusted during placement and in subsequent interventions.
The utility of guide catheter extensions (GCE) in coronary interventions and specifically chronic total occlusion (CTO) interventions
Authors: Poommipanit Paul , Tashtish Nour 
Published: March 2024

Key points
Guide catheter extensions (GCE) are associated with improved success in chronic total occlusions interventions.
GCE can be associated with a higher risk of complications, although found to not be statistically significant in this large multicenter registry.
Care must be exercised in the use of GCE to ensure a successful intervention and limit rates of complications.
Trans‐catheter repair of tricuspid regurgitation following left heart valve surgery: Expansion of the interventionist portfolio
Authors: Hanson Ivan , Goldstein James A. 
Published: March 2024

Key points
Tricuspid regurgitation (TR) is highly prevalent and leads to poor outcomes if left untreated.
Surgical treatment of TR, if indicated, is generally recommended at the time of left heart valve surgery.
Patients with only mild TR not meeting indications for concomitant tricuspid repair/replacement may develop progressive TR requiring treatment.
A strategy of trans‐catheter tricuspid valve replacement in select patients with prior left heart valve surgery is feasible
Local coordination and electronic interactions of Pd/MXene via dual‐atom codoping with superior durability for efficient electrocatalytic ethanol oxidation
Authors: Chen Zhangxin , Jing Fan , Luo Minghui , Wu Xiaohui , Fu Haichang , Xiao Shengwei , Yu Binbin , Chen Dan , Xiong Xianqiang , Jin Yanxian 
Published: March 2024

AbstractCatalyst design relies heavily on electronic metal‐support interactions, but the metal‐support interface with an uncontrollable electronic or coordination environment makes it challenging. Herein, we outline a promising approach for the rational design of catalysts involving heteroatoms as anchors for Pd nanoparticles for ethanol oxidation reaction (EOR) catalysis. The doped B and N atoms from dimethylamine borane (DB) occupy the position of the Ti3C2 lattice to anchor the supported Pd nanoparticles. The electrons transfer from the support to B atoms, and then to the metal Pd to form a stable electronic center. A strong electronic interaction can be produced and the d‐band center can be shifted down, driving Pd into the dominant metallic state and making Pd nanoparticles deposit uniformly on the support. As‐obtained Pd/DB–Ti3C2 exhibits superior durability to its counterpart (∼14.6% retention) with 91.1% retention after 2000 cycles, placing it among the top single metal anodic catalysts. Further, in situ Raman and density functional theory computations confirm that Pd/DB–Ti3C2 is capable of dehydrogenating ethanol at low reaction energies.