bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypoxic Exosomal circPLEKHM1‐Mediated Crosstalk between Tumor Cells and Macrophages Drives Lung Cancer Metastasis
Authors: Wang Dongliang , Wang Shuoer , Jin Mingming , Zuo Yan , Wang Jianpeng , Niu Ya , Zhou Qian , Chen Jiwei , Tang Xinru , Tang Wenxuan , Liu Xiyu , Yu Hang , Yan Wangjun , Wei Huan‐Huan , Huang Gang , Song Shaoli , Tang Shuang 
Published: March 2024

AbstractIntercellular communication often relies on exosomes as messengers and is critical for cancer metastasis in hypoxic tumor microenvironment. Some circular RNAs (circRNAs) are enriched in cancer cell‐derived exosomes, but little is known about their ability to regulate intercellular communication and cancer metastasis. Here, by systematically analyzing exosomes secreted by non‐small cell lung cancer (NSCLC) cells, a hypoxia‐induced exosomal circPLEKHM1 is identified that drives NSCLC metastasis through polarizing macrophages toward to M2 type. Mechanistically, exosomal circPLEKHM1 promoted PABPC1‐eIF4G interaction to facilitate the translation of the oncostatin M receptor (OSMR), thereby promoting macrophage polarization for cancer metastasis. Importantly, circPLEKHM1‐targeted therapy significantly reduces NSCLC metastasis in vivo. circPLEKHM1 serves as a prognostic biomarker for metastasis and poor survival in NSCLC patients. This study unveils a new circRNA‐mediated mechanism underlying how cancer cells crosstalk with macrophages within the hypoxic tumor microenvironment to promote metastasis, highlighting the importance of exosomal circPLEKHM1 as a prognostic biomarker and therapeutic target for lung cancer metastasis.
A High‐Density Hydrogen Bond Locking Strategy for Constructing Anisotropic High‐Strength Hydrogel‐Based Meniscus Substitute
Authors: Zhang Qian , Yang Xuxuan , Wang Kuan , Xu Ziyang , Liu Wenguang 
Published: March 2024

AbstractMimicking anisotropic features is crucial for developing artificial load‐bearing soft tissues such as menisci). Here, a high‐density hydrogen bond locking (HDHBL) strategy, involving preloading a poly(N‐acryloylsemicarbazide) (PNASC) hydrogel with an aqueous solution containing a hydrogen bond breaking agent, followed by water exchange, to fabricate anisotropic high‐strength hydrogels are proposed. During this process, multiple high‐density hydrogen bonds of the PNASC network are re‐established, firmly freezing oriented molecular chains, and creating a network with an anisotropic microstructure. The resulting anisotropic hydrogels exhibit superior mechanical properties: tensile strength over 9 MPa, Young's modulus exceeding 120 MPa along the orientation direction, and fatigue thresholds exceeding 1900 J m−2. These properties meet the mechanical demands for load‐bearing tissue substitutes compared to other reported anti‐fatigue hydrogels. This strategy enables the construction of an anisotropic meniscal scaffold composed of circumferentially oriented microfibers by preloading a digital light processing‐3D printed PNASC hydrogel‐based wedge‐shaped construct with a resilient poly(N‐acryloyl glycinamide) hydrogel. The 12‐week implantation of a meniscus scaffold in rabbit knee joints after meniscectomy demonstrates a chondroprotective effect on the femoral condyle and tibial plateau, substantially ameliorating the progression of osteoarthritis. The HDHBL strategy enables the fabrication of various anisotropic polymer hydrogels, broadening their scope of application.
Undiagnosed Disease Network collaborative approach in diagnosing rare disease in a patient with a mosaic CACNA1D variant
Authors: Ezell Kimberly M. , Tinker Rory J. , Furuta Yutaka , Gulsevin Alican , Bastarache Lisa , Hamid Rizwan , Cogan Joy D. , Rives Lynette , Neumann Serena , Corner Brian , Kozuria Mary , Phillips John A. ,   the Undiagnosed Diseases Network
Published: March 2024

AbstractThe Undiagnosed Disease Network (UDN) is comprised of clinical and research experts collaborating to diagnose rare disease. The UDN is funded by the National Institutes of Health and includes 12 different clinical sites (About Us, 2022). Here we highlight the success of collaborative efforts within the UDN Clinical Site at Vanderbilt University Medical Center (VUMC) in utilizing a cohort of experts in bioinformatics, structural biology, and genetics specialists in diagnosing rare disease. Our UDN team identified a de novo mosaic CACNA1D variant c.2299T>C in a 5‐year‐old female with a history of global developmental delay, dystonia, dyskinesis, and seizures. Using a collaborative multidisciplinary approach, our VUMC UDN team diagnosed the participant with Primary Aldosteronism, Seizures, and Neurologic abnormalities (PASNA) OMIM: 615474 due to a rare mosaic CACNA1D variant (O'Neill, 2013). Interestingly, this patient was mosaic, a phenotypic trait previously unreported in PASNA cases. This report highlights the importance of a multidisciplinary approach in diagnosing rare disease.
Copper(I)‐Catalyzed Enantioselective Synthesis of Chiral Cyclohexenylallenes and Transfer of Axial to Center Chirality by Ytterbium (III) Catalyzed [4+2] Cycloaddition
Authors: Satyanarayana Iddum , Mohan Lakavathu , Periasamy Mariappan 
Published: March 2024

AbstractChiral cyclohexenylallenes are readily accessed from 1‐enynes, aldehydes and chiral secondary amines in good yields (up to 79 % yield) with excellent enantioselectivities (up to 99 % ee), through CuI catalyzed the formation of chiral propargylamine intermediates. Subsequent Yb(OTf)3 catalyzed [4+2] cycloaddition reaction of cyclohexylallenes with the dienophiles tetracyanoethylene and diethyl acetylenedicarboxylate afforded the corresponding bicyclic products in 69–75 % yield and 84–90 % ee.
Visible‐Light Activation of Diorganyl Bis(pyridylimino) Isoindolide Aluminum(III) Complexes and Their Organometallic Radical Reactivity
Authors: Wenzel Jonas O. , Werner Johannes , Allgaier Alexander , van Slageren Joris , Fernández Israel , Unterreiner Andreas‐Neil , Breher Frank 
Published: March 2024

AbstractWe report on the synthesis and characterization of a series of (mostly) air‐stable diorganyl bis(pyridylimino) isoindolide (BPI) aluminum complexes and their chemistry upon visible‐light excitation. The redox non‐innocent BPI pincer ligand allows for efficient charge transfer homolytic processes of the title compounds. This makes them a universal platform for the generation of carbon‐centered radicals. The photo‐induced homolytic cleavage of the Al−C bonds was investigated by means of stationary and transient UV/Vis spectroscopy, spin trapping experiments, as well as EPR and NMR spectroscopy. The experimental findings were supported by quantum chemical calculations. Reactivity studies enabled the utilization of the aluminum complexes as reactants in tin‐free Giese‐type reactions and carbonyl alkylations under ambient conditions, which both indicated radical‐polar crossover behavior. A deeper understanding of the physical fundamentals and photochemical process was provided, furnishing in turn a new strategy to control the reactivity of bench‐stable aluminum organometallics.
Hydrogen Evolution in Neutral Media by Differential Intermediate Binding at Charge‐Modulated Sites of a Bimetallic Alloy Electrocatalyst
Authors: Maji Mamoni , Dutta Supriti , Jena Rohan , Dey Anupam , Maji Tapas Kumar , Pati Swapan K. , Bhattacharyya Sayan 
Published: March 2024

AbstractThe energy barrier to dissociate neutral water has been lowered by the differential intermediate binding on the charge‐modulated metal centers of Co85Mo15 sheets supported on Ni‐foam (NF), where the overpotential for hydrogen evolution reaction (HER) in 1 M phosphate buffer solution (PBS) is only 50±9 mV at −10 mA cm−2. It has a turnover frequency (TOF) of 0.18 s−1, mass activity of 13.2 A g−1 at −200 mV vs. reversible hydrogen electrode (RHE), and produces 16 ml H2 h−1 at −300 mV vs. RHE, more than double that of 20 % Pt/C. The Moδ+ and Coδ− sites adsorb OH*, and H*, respectively, and the electron injection from Co to H−O−H cleaves the O−H bond to form the Mo−OH* intermediate. Operando spectral analyses indicate a weak H‐bonded network for facilitating the H2O*/OH* transport, and a potential‐induced reversal of the charge density from Co to the more electronegative Mo, because of the electron withdrawing Co−H* and Mo−OH* species. Co85Mo15/NF can also drive the complete electrolysis of neutral water at only 1.73 V (10 mA cm−2). In alkaline, and acidic media, it demonstrates a Pt‐like HER activity, accomplishing −1000 mA cm−2 at overpotentials of 161±7, and 175±22 mV, respectively.
In‐depth investigation and industry plan for enhancing surface finishing of 3D printed polymer composite components: A critical review
Authors: Karimi Naeim , Bozorgnia Tabary Seyed Amir Ali , Fayazfar Haniyeh (Ramano) 
Published: March 2024

AbstractAdditive manufacturing (AM) is pivotal in modern manufacturing, allowing diverse materials to create functional parts. Polymer composites, with superior properties like enhanced mechanics and conductivity, are highly regarded. Fused deposition modeling (FDM) is a favored, cost‐effective AM technique. Despite popularity, FDM struggles with poor surface quality, impacting final part properties. This challenge affects composite part performance, prompting a need for surface quality enhancements in AM processes, like FDM. The review explores essential post‐treatments required to optimize composite parts for various applications. It examines surface finishing techniques for FDM 3D printed polymeric composites, categorizing them into chemical, thermal, and physical methods. Recent research is extensively analyzed, offering a comprehensive guide for academia and industry. The review covers diverse aspects, including properties, surface morphology, electrical conductivity, and mechanical properties pre‐ and post‐finishing methods. Additionally, it summarizes leading companies worldwide providing surface finishing services for 3D printed polymer parts. Discussions on future trends, challenges, and research gaps in this field are included, emphasizing its significance in refining surface finishing methods for FDM 3D‐printed polymeric composites and encouraging broader industrial adoption of AM. This overview serves as a crucial roadmap for engineers, scientists, customers, and companies interested in surface finishing services.
Ligand‐free MnBr2‐Catalyzed Chemo‐ and Stereoselective Hydroboration of Terminal Alkynes
Authors: Pawar Rameshwar B. , Karmur Mital H. , Punji Benudhar 
Published: March 2024

AbstractDeveloping simple and benign protocols for synthesizing alkenylboronates is crucial as they are synthetically valuable compounds in various organic transformations. In this work, we report a straightforward ligand‐free protocol for synthesizing alkenylboronates via atom‐economical hydroboration of alkynes with HBpin catalyzed by a manganese salt. The reaction shows a high level of chemo and regioselectivity for the terminal alkynes and exclusively produces E‐selective alkenylboronates. The hydroboration scope is vast, with the resilience of a range of synthetically beneficial functionalities, such as halides, ether, alkenyl, silyl and thiophenyl groups. This reaction proceeds through the involvement of a metal‐hydride intermediate. The developed alkenylboronate can be smoothly converted to useful C‐C, C‐N and C‐I bond‐forming reactions.
Sequential extraction of hesperidin, pectin, lignin, and cellulose from orange peels: towards valorization of agro‐waste
Authors: de Castro Symone C. , Stanisic Danijela , Tasic Ljubica 
Published: March 2024

AbstractTo maximize the reuse of citrus fruit processing waste, which is generated in large amounts due to juice production, this work shows the results of a new renewable approach for the extraction of high‐added‐value compounds present in orange biomass, specifically in orange peels (OP). The method is based on the use of diluted Ca2+, acid, and base solutions for the extraction of hesperidin (HSD), pectin, lignin, and cellulose sequentially, without generating any organic solid waste at the end, and without the need for air drying the starting biomass. Good yields of hesperidin were recovered (~1.56%), with excellent purity (up to 95%), as well as lignin (yield ~2.2%) and cellulose (yield ~12%). Pectin obtained by this process showed a high amount of galacturonic acid (~88%) and a low degree of methylation (~16%). All products were thoroughly characterized by applying Fourier transform infrared (FTIR) spectroscopy and solid‐state cross‐polarization/magic angle spinning carbon‐13 nuclear magnetic resonance spectroscopy (CP/MAS 13C‐NMR). The products recovered from OP can be applied in a wide array of fields, such as food, medicine, and pharmaceuticals, among others.
Unique biomedical application of fluorescence derivatization based on palladium‐catalyzed coupling reactions for HPLC analysis of pharmaceuticals and biomolecules
Authors: El‐Maghrabey Mahmoud , Kishikawa Naoya , Kuroda Naotaka 
Published: March 2024

AbstractPalladium‐catalyzed coupling reactions are versatile and powerful tools for the construction of carbon–carbon bonds in organic synthesis. Although these reactions have favorable features that proceed selectively in mild reaction conditions using aqueous organic solvents, no attention has been given to their application in the field of biomedical analysis. Therefore, we focused on these reactions and evaluated the scope and limitations of their analytical performance. In this review, we describe the pros and cons and future trends of fluorescence derivatization of pharmaceuticals and biomolecules based on palladium‐catalyzed coupling reactions such as Suzuki–Miyaura coupling, Mizoroki–Heck coupling, and Sonogashira coupling reactions for HPLC analysis.
Enhanced Ceftriaxone Determination using a Glassy Carbon Electrode Modified with a Cr‐Based Metal‐Organic Framework Film Stabilized by β‐Cyclodextrin Polymer
Authors: Bougna Honorine Hortense Tchoumi , Dongmo Liliane Medonbou , Jiokeng Sherman Lesly Zambou , Ntep Tobie Matemb Ma , Mbokou Serge Foukmeniok , Janiak Christoph , Djouaka Rousseau , Tonle Ignas Kenfack 
Published: March 2024

AbstractA selective amperometric sensor was developed to detect the antibiotic ceftriaxone (CEF) using a composite film of poly(β‐cyclodextrin) and a metal‐organic framework (MOF). The sensor was obtained by electro‐polymerizing β‐cyclodextrin (β‐CD) onto a glassy carbon electrode (GCE) previously coated with a MIL‐101(Cr) MOF film. This design prevents MOF particles’ loss during extended electrode use. The MOF was structurally characterized using scanning electron microscopy, X‐ray diffraction, N2 adsorption‐desorption isotherms (BET method) and FTIR spectroscopy. Electrochemical impedance spectroscopy and cyclic voltammetry were used to assess the GCE/MIL‐101(Cr)‐pβ‐CD sensor, revealing reduced charge transfer resistance and enhanced sensitivity to CEF. Differential pulse voltammetry was then employed for CEF determination. Under optimized conditions, the sensor exhibited high sensitivity, a broad linear working range of 0.5 to 6 μM (R2=0.991), and a low detection limit of 0.34±0.02 μM (0.19±0.01 mg L−1). The selectivity of the sensor was evaluated in the presence of potential interferences such as organic acids, antibiotics and selected ions expected to affect the CEF signal. In an application to quantify a pharmaceutical formulation, the sensor displayed an impressive recovery rate of 98.3 % compared to results obtained using high‐performance liquid chromatography.
Biopolymers‐Based Proton Exchange Membranes For Fuel Cell Applications: A Comprehensive Review
Authors: Abouricha Souhaib , Aziam Hasna , Noukrati Hassan , Sel Ozlem , Saadoune Ismael , Lahcini Mohammed , Youcef Hicham Ben 
Published: March 2024

AbstractNafion has been dominating the proton exchange membrane (PEM) market because of its adequacy for the proton exchange operation. However, because of its high cost, low performance at low relative humidity, and finally environmental unfriendliness, researchers have been developing and working on Nafion alternatives. In this review, we assess how biopolymers present an opportunity as an eco‐friendly material able to replace the conventional Nafion membrane in proton exchange membrane fuel cells (PEMFC). Chitosan and cellulose were the most studied in literature owing to their abundance in nature, low cost, and high affinity for water, which are sought‐after properties for PEM. In addition to that, their ease of modification through their hydroxyl or amine groups represents an opportunity to further enhance their characteristics and thus meet the criteria of diverse applications. The use of biopolymers as an adequate PEM is facing many challenges. Having a practical proton conductivity and securing the physicochemical stability of the biopolymers in fuel cell operating conditions are two of the most important challenges to overcome. Promising strategies to simultaneously address these challenges such as crosslinking, making interpenetrated networks, and making blends and composites are reported.
Pressure‐Induced Structural, Optical and Magnetic Modifications in Lanthanide Single‐Molecule Magnets
Authors: Pointillart Fabrice , Le Guennic Boris , Cador Olivier 
Published: March 2024

AbstractLanthanide Single‐Molecule Magnets are fascinating objects that break magnetic performance records with observable magnetic bistability at the boiling temperature of liquid nitrogen, paving the way for potential applications in high‐density data storage. The switching of lanthanide SMM has been successfully achieved using several external stimuli such as redox reaction, pH titration, light irradiation or solvation/desolvation thanks to the high sensitivity of the magnetic anisotropy to any structural change in the lanthanide surrounding. Nevertheless, the use of applied high pressure as an external stimulus is largely underused, especially considering that it can be combined with high pressure X‐ray diffraction to establish a complementary structure‐property relationship. This Concept article summarizes the few relevant examples of investigations of lanthanide SMMs under applied high pressure, provides conclusions on the effect of such stimulus on molecular structures and magnetic anisotropy, and finally draws perspective on the future development of magnetic measurements under applied pressure.
Optimized Recombinant Expression and Purification of the SARS‐CoV‐2 Polymerase Complex
Authors: Podadera Ana , Campo Lucas , Rehman Fasih , Kolobaric Nikola , Zutic Adriana , Ng Kenneth K‐S. 
Published: March 2024

AbstractAn optimized protocol has been developed to express and purify the core RNA‐dependent RNA polymerase (RdRP) complex from the severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2). The expression and purification of active core SARS‐CoV‐2 RdRp complex is challenging due to the complex multidomain fold of nsp12, and the assembly of the multimeric complex involving nsp7, nsp8, and nsp12. Our approach adapts a previously published method to express the core SARS‐CoV‐2 RdRP complex in Escherichia coli and combines it with a procedure to express the nsp12 fusion with maltose‐binding protein in insect cells to promote the efficient assembly and purification of an enzymatically active core polymerase complex. The resulting method provides a reliable platform to produce milligram amounts of the polymerase complex with the expected 1:2:1 stoichiometry for nsp7, nsp8, and nsp12, respectively, following the removal of all affinity tags. This approach addresses some of the limitations of previously reported methods to provide a reliable source of the active polymerase complex for structure, function, and inhibition studies of the SARS‐CoV‐2 RdRP complex using recombinant plasmid constructs that have been deposited in the widely accessible Addgene repository. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Expression and production of SARS‐CoV‐2 nsp7, nsp8, and nsp12 in E. coli cellsSupport Protocol: Establishment and maintenance of insect cell culturesBasic Protocol 2: Generation of recombinant baculovirus in Sf9 cells and production of nsp12 fusion protein in T. ni cellsBasic Protocol 3: Purification of the SARS‐CoV‐2 core polymerase complex
A Step‐by‐Step Guide for the Production of Recombinant Fluorescent TAT‐HA‐Tagged Proteins and their Transduction into Mammalian Cells
Authors: Anny Christer Abou , Nouaille Sébastien , Fauré Régis , Schulz Céline , Spriet Corentin , Huvent Isabelle , Biot Christophe , Lefebvre Tony 
Published: March 2024

AbstractInvestigating the function of target proteins for functional prospection or therapeutic applications typically requires the production and purification of recombinant proteins. The fusion of these proteins with tag peptides and fluorescently derived proteins allows the monitoring of candidate proteins using SDS‐PAGE coupled with western blotting and fluorescent microscopy, respectively. However, protein engineering poses a significant challenge for many researchers. In this protocol, we describe step‐by‐step the engineering of a recombinant protein with various tags: TAT‐HA (trans‐activator of transduction‐hemagglutinin), 6×His and EGFP (enhanced green fluorescent protein) or mCherry. Fusion proteins are produced in E. coli BL21(DE3) cells and purified by immobilized metal affinity chromatography (IMAC) using a Ni‐nitrilotriacetic acid (NTA) column. Then, tagged recombinant proteins are introduced into cultured animal cells by using the penetrating peptide TAT‐HA. Here, we present a thorough protocol providing a detailed guide encompassing every critical step from plasmid DNA molecular assembly to protein expression and subsequent purification and outlines the conditions necessary for protein transduction technology into animal cells in a comprehensive manner. We believe that this protocol will be a valuable resource for researchers seeking an exhaustive, step‐by‐step guide for the successful production and purification of recombinant proteins and their entry by transduction within living cells. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: DNA cloning, molecular assembly strategies, and protein productionBasic Protocol 2: Protein purificationBasic Protocol 3: Protein transduction in mammalian cells
Lonesome plants: How isolation affects seed set of a threatened dioecious shrub
Authors: García‐Guzmán Patricio , Carvajal Danny E. , Carozzi‐Figueroa Giovanni , Loayza Andrea P. 
Published: March 2024

AbstractPlant reproductive failure is a critical concern for conserving rare and endangered species that typically have low‐density and sparse populations. One important factor contributing to reproductive failure is the spatial arrangement of plants within a population, which can lead to isolation and negatively affect seed production, particularly in obligate outcrossers. Additionally, plant size can compound this effect, influencing seed production via multiple processes. Here, we investigate how spatial distribution and size influence the reproductive success of Vasconcellea chilensis, an endemic‐threatened papaya species in Chile. We first examined whether V. chilensis can produce seeds via apomixis using pollinator exclusion experiments. We then used Spatial Point Pattern Analysis (SPPA) in three populations to explore the spatial arrangement of plants. Finally, we assessed whether plant size and neighbor distance influence the reproductive success V. chilensis is a dioecious shrub unable to produce fruits through apomixis. The SPPA revealed significant clustering of female and male plants at different spatial scales, indicating a non‐random distribution. Moreover, a significant spatial association between the sexes was observed. In two populations, closer proximity to male plants was linked to higher seed production. Our study revealed that the reproductive system of V. chilensis is susceptible to distance‐dependent reproductive failure due to pollen limitation. While the species' spatial structure may partially mitigate this risk, female plants isolated from male counterparts will likely experience reduced seed set.