bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic nomogram for patients undergoing oesophagectomy for adenocarcinoma of the oesophagus or gastro-oesophageal junction
Authors: Lagarde S M , Reitsma J B , de Castro S M M , ten Kate F J W , Busch O R C , van Lanschot J J B 
Published: June 2007

AbstractBackgroundTumour node metastasis (TNM) staging predicts survival on the basis of the pathological extent of a tumour. The aim of this study was to develop a prognostic model with improved survival prediction after oesophagectomy.MethodsConsecutive patients who had potentially curative oesophagectomy for adenocarcinoma of the oesophagus or gastro-oesophageal junction were included. Cox regression analyses were performed to examine the association between risk factors and time to death from oesophageal cancer. The concordance index, calculated after bootstrapping, was used to measure accuracy. A nomogram was designed for use in clinical practice.ResultsOesophageal cancer-specific survival rates for the 364 included patients who underwent oesophagectomy between 1993 and 2003 were 75·8, 54·9 and 39·2 per cent at 1, 2 and 5 years respectively. A prognostic model using all prognostic variables outperformed TNM staging (concordance index 0·79 versus 0·68 respectively; P &lt; 0·001). A reduced model derived after backward elimination, containing only T stage, lymph node ratio and extracapsular lymph node involvement, also outperformed TNM staging (concordance index 0·77; P &lt; 0·001).ConclusionA prognostic model developed to predict disease-specific survival after oesophagectomy was superior to TNM staging. More reliable prognostic information might lead to different approaches to patient follow-up.
Enucleation of pancreatic neoplasms
Authors: Crippa S , Bassi C , Salvia R , Falconi M , Butturini G , Pederzoli P 
Published: June 2007

AbstractBackgroundStandard resections for benign and borderline neoplasms of the pancreas are associated with a significant risk of long-term functional impairment, whereas enucleation preserves healthy parenchyma and pancreatic function. The aim of this study was to evaluate postoperative and long-term oncological and functional results after pancreatic enucleation.MethodsData collected prospectively from 61 consecutive patients who underwent pancreatic enucleation were analysed.ResultsThere were no deaths. A clinically significant pancreatic fistula was reported in 14 patients (23 per cent), and five patients (8 per cent) had a further operation for fistula-related complications. The most common indication for surgery was endocrine neoplasm (38 patients; 62 per cent) and two patients (3 per cent) had a final histopathological diagnosis of malignant neoplasm. At a median follow-up of 61 months no patient had developed tumour recurrence or exocrine insufficiency. Two elderly patients developed non-insulin-dependent diabetes.ConclusionEnucleation is an effective procedure for the radical treatment of benign and borderline neoplasms of the pancreas, with good long-term outcomes.
Signal suppression/enhancement in HPLC‐ESI‐MS/MS from concomitant medications
Authors: Leverence Rachael , Avery Michael J. , Kavetskaia Olga , Bi Honggang , Hop Cornelis E. C. A. , Gusev Arkady I. 
Published: June 2007

AbstractThis paper presents a study of the signal suppression and enhancement effects in assays based on HPLC‐ESI‐MS/MS detection. The major focus was to investigate the effect of signal suppression/enhancement of typical co‐administered (concomitant) medications, i.e. naproxen and ibuprofen. The results demonstrate that the analyte and internal standard can experience signal enhancement up to a factor of ca 2.9 if the test analyte or internal standard co‐elute with concomitant. Experimental results also demonstrate that the analyte and internal standard signal increased by a factor of ca 2.0 in the negative ion mode at physiological relevant levels of naproxen (100 µg/mL) and by a factor of ca 1.6 in the negative ion mode at physiological relevant level of ibuprofen (10 µg/mL) in both neat and plasma samples. Signal enhancement significantly increased when concomitant medications ionized in the same ion mode as the analyte and internal standard. To overcome signal enhancement or potential suppression from concomitant medications, a comprehensive HPLC method needs to be developed with sufficient separation of concomitant medication from the analyte and internal standard. Other means to reduce signal enhancement or potential suppression include switching ionization polarity and performing comprehensive sample clean‐up to remove concomitant medications before analysis. Copyright © 2007 John Wiley & Sons, Ltd.
Simultaneous determination of 17 antiretroviral drugs in human plasma for quantitative analysis with liquid chromatography–tandem mass spectrometry
Authors: Jung Byung Hwa , Rezk Naser L. , Bridges Arlene S. , Corbett Amanda H. , Kashuba Angela D. M. 
Published: June 2007

AbstractThis work describes a sensitive method for the simultaneous determination of 17 antiretroviral drugs including nucleoside analog reverse transcriptase inhibitors, non‐nucleoside analog reverse transcriptase inhibitors, protease inhibitors and a nucleotide analog reverse transcriptase inhibitor in 50 µL of human plasma. This method employed high‐performance liquid chromatography–tandem mass spectrometry with electrospray ionization. The analytes were monitored in multiple reaction monitoring mode and the polarity was switched from positive to negative to positive to detect all compounds after a single injection. A combination of liquid–liquid extraction and protein precipitation was used to extract all compounds, with at least 75% recovery for all analytes. Within‐ and between‐day accuracies were at least 85% for 1–500 ng/mL for nelfinavir, indinavir and abacavir, 10–500 ng/mL for didanosine and stavudine and 5–500 ng/mL for all other compounds. This method is very effective for quantifying and screening antiretroviral drugs in clinical samples with limited (50 µL) volumes. Copyright © 2007 John Wiley & Sons, Ltd.
Capillary electrophoresis of signaling molecules
Authors: Suresh Babu C. V. , Song Eun Joo , Babar Sheikh Md. Enayetul , Yoo Young Sook 
Published: June 2007

AbstractThe emerging field of quantitative systems biology uses high‐throughput bioanalytical measurements to gain a deeper understanding of biological phenomena. With the advent of instrumentation platforms, capillary electrophoresis spans a very wide range of biological applications. This short article focuses on the exploitation of capillary electrophoresis for the systems‐level analysis of cell signaling molecules. Copyright © 2007 John Wiley & Sons, Ltd.
Liquid chromatography–tandem mass spectrometry method for identification and quantification of two biologically active polyisoprenylated benzophenones, isoxanthochymol and camboginol, in Garcinia species
Authors: Chattopadhyay Sunil K. , Kumar Satyanshu 
Published: June 2007

AbstractA sensitive liquid chromatography/electrospray ionization tandem mass spectrometrical (LC/ESI‐MS/MS) method was developed for simultaneous identification and quantification of two polyisoprenylated benzophenones, isoxanthochymol and camboginol, in the extracts of the fruit rinds, stem bark, seed and leaves of Garcinia indica and in the fruit rinds of Garcinia cambogia. The separation of isoxanthochymol and camboginol was achieved on an RP‐8 column using the solvent system consisting of a mixture of acetonitrile–water (80:20) and methanol–acetic acid (99.0:1.0) as a mobile phase in a gradient elution mode. A multiple reaction monitoring (MRM) method was developed for quantification of isoxanthochymol and camboginol in the above extracts of Garcinia species. Based on a signal‐to‐noise ratio of 3, the limits of detection in MRM mode for isoxanthochymol and camboginol were 2.0 and 5.0 ng/mL respectively. The method was validated in terms of linearity, accuracy and precision for 6 days. The method developed was found to be useful for identification and quantification of isoxanthochymol and camboginol in the extracts of the fruit rinds, stem bark, seed and leaves of Garcinia indica and in the fruit rinds of Garcinia cambogia. Copyright © 2007 John Wiley & Sons, Ltd.
Fibularis tertius: Revisiting the anatomy
Authors: Rourke K. , Dafydd H. , Parkin I.G. 
Published: June 2007

AbstractFibularis tertius (FT) may be used during reconstructive surgery and muscle transposition with retention of function. The muscle was examined in both lower limbs of 41 cadavers. Measurements were made of muscle belly length and width, tendon length and width, and the size of the origin on the fibula. Tendon insertion, nerve and blood supplies were also examined. FT was absent in five (6.1%) lower limbs of three (7.3%) subjects. The size of its origin demonstrated inter‐ and intra‐individual variation. FT arose from the distal fibula and on average occupied (28.4 ± 9.1)% (mean ± S. D.) of the total shaft length. In all cases the tendon inserted into the dorsal surface of the shafts of both the fourth and fifth metatarsals. A small nerve branch consistently arose from the deep fibular nerve near the origin of extensor digitorum longus. The nerve ran parallel to the length of this muscle, between it and extensor hallucis longus, before piercing FT. Anatomy textbooks describe FT as inserting into the fifth metatarsal only. This study, supported by data from previous reports, suggests that the “textbook” accounts of FT should be updated to record that most commonly its tendon reaches both the fourth and fifth metatarsals. Clin. Anat. 20:946–949, 2007. © 2007 Wiley‐Liss, Inc.
Assessing macroscopic and microscopic indicators of osteoarthritis in the distal interphalangeal joints: A cadaveric study
Authors: Bentley Brian S. , Hill Robert V. 
Published: June 2007

AbstractOsteoarthritis is manifested both by macroscopically visible lesions and by specific histological indicators. Although traditional views of the disease process invoke physical abrasion of joint surfaces, recent studies indicate that tissue‐level changes may precede grossly visible lesions of articular cartilage. This study investigates the association between gross and histological indicators of osteoarthritis at the manual interphalangeal joints, and examines a sequence of events that may lead to the onset of cartilage degeneration. Interphalangeal joints from the hands of nine cadavers were dissected, of which 52 joints were collected and further evaluated. Gross degradation of the proximal articular surface was graded on a scale of 0–3 (with 0 representing normal cartilage with no visible lesions). Osteoarthritic lesions were found in 86% of specimens and showed no preferential occurrence between males and females or right and left hands. Histological analysis indicated that known microscopic indicators of osteoarthritis always occur in Grade 1–3 specimens, but can also be recognized in some macroscopically normal specimens. Many macroscopically normal specimens exhibited chondrocyte clustering (28.6%) and/or tide mark irregularities (57%), indicating that these features are most likely the earliest to develop in the progression of osteoarthritis. It is possible that the initiating etiology is thickening of the subchondral bone, but this was not directly observed. Results indicate significant thinning of the cartilage as macroscopic degradation progresses. Our study supports, with slight modification, a previously proposed cascade of histological changes that may ultimately lead to the physical destruction of articular cartilage. Clin. Anat. 20:799–807, 2007. © 2007 Wiley‐Liss, Inc.
A descriptive tissue evaluation at maxillary interradicular sites: Implications for orthodontic mini‐implant placement
Authors: Lim Won Hee , Lee Soo Kyung , Wikesjö Ulf Me , Chun Youn Sic 
Published: June 2007

AbstractFew studies have evaluated interradicular anatomy for hard and soft tissue thickness. Because interradicular sites are common regions for mini‐implant placement for orthodontic anchorage, the purpose of this study was to provide a guideline to indicate the best location for mini‐implants as it relates to the thickness of cortical bone and soft tissue, and to the height of the attached gingival field. CT images from 15 men and 15 women (mean age 27 years, range 23–35 years) were used to evaluate the buccal interradicular cortical bone thickness from and mesial to the central incisor to the 1st molar. To record soft tissue depth at the site of assessment for cortical bone thickness, the mucosa was pierced with a #15 endodontic K‐file until the attached rubber stop rested on the mucosa. The height of attached gingiva was measured at the mid‐aspect of each tooth using a caliper. There were no significant differences in cortical bone thickness within interradicular sites except for the 2nd premolar/1st molar site. There were also no significant differences in soft tissue thickness within interradicular sites except for the lateral incisor/canine and 2nd premolar/1st molar sites. The height of attached gingiva was greater in the anterior compared to the posterior region and was shortest in the premolar region. Given the limits of this study, mini‐implants for orthodontic anchorage may be well placed with equivalent bone‐implant contact anywhere within the zone of attached gingiva up to 6 mm apical to the alveolar crest with adequate interradicular space. Clin. Anat. 20:760–765, 2007. © 2007 Wiley‐Liss, Inc.
Superficial palmar communications between the ulnar and median nerves in Turkish cadavers
Authors: Tagil Süleyman Murat , Bozkurt Mehmet Cem , Özçakar Levent , Ersoy Mehmet , Tekdemi̇r İbrahim , Elhan Alaittin 
Published: June 2007

AbstractThe superficial palmar communicating branch between the ulnar and median nerves, the ramus communicans (RC), has been investigated by anatomical dissections in 30 hands of 15 cadavers in the Turkish population. A communicating branch between the ulnar and median nerves was found in 18 hands (60%). It was classified into 4 types: Type 1–RC emerging from the ulnar 4th common digital nerve (CDN) and uniting with the median 3rd CDN, Type 2–RC emerging from the median 3rd CDN and uniting with the ulnar 4th CDN, Type 3–RC extending horizontally between the 3rd and 4th CDN, Type 4–a plexiform RC formed by multiple twigs. Twelve hands (40%) showed the Type 1 arrangement, one was Type 2 (3.3%), two were Type 3 (6.7%) and three (10%) were Type 4, based on the classification devised by Meals and Shaner ([;1983] J Hand Surg Am 8:411–414) and modified by Bas and Kleinert ([1999] J Hand Surg Am 24:1171–1184). Knowledge of the anatomical characteristics of this branch is important for surgical interventions, especially in carpal tunnel release operations. Clin. Anat. 20:795–798, 2007. © 2007 Wiley‐Liss, Inc.
Structural Ramification for Acetyl‐Lysine Recognition by the Bromodomain of Human BRG1 Protein, a Central ATPase of the SWI/SNF Remodeling Complex
Authors: Singh Mahavir , Popowicz Grzegorz M. , Krajewski Marcin , Holak Tad A. 
Published: June 2007

AbstractBromodomains represent an extensive family of evolutionarily conserved domains that are found in many chromatin‐associated proteins such as histone acetyltransferases (HAT) and subunits of ATP‐dependent chromatin‐remodeling complexes. These domains are associated with acetylated lysine residues that bind both in vivo and in vitro; for example, they bind to the N‐acetylated lysines of the histone tail of nucleosomes. In this report, we determined the structure of the bromodomain from human brahma‐related gene 1 (BRG1) protein, a subunit of an ATP‐dependent switching/sucrose nonfermenting (SWI/SNF) remodeling complex, and have also characterized its in vitro interaction with N‐acetylated lysine peptides from histones. In addition to a typical all‐α‐helical fold that was observed in the bromodomains, we observed for the first time a small β‐sheet in the ZA loop region of the BRG1 protein. The BRG1 bromodomain exhibited binding, albeit weak, to acetylated peptides that were derived from histones H3 and H4. We have compared the acetyl‐lysine binding sites of BRG1 bromodomain with the yGCN5 (general control of amino acid biosynthesis). By modeling the acetylated‐lysine peptide into the BRG1 bromodomain structure, we were able to explain the weak binding of acetylated‐lysine peptides to this bromodomain.
Mutational Analysis of the Myxovirescin Biosynthetic Gene Cluster Reveals Novel Insights into the Functional Elaboration of Polyketide Backbones
Authors: Simunovic Vesna , Müller Rolf 
Published: June 2007

AbstractIt has been proposed that two acyl carrier proteins (ACPs)—TaB and TaE—and two 3‐hydroxy‐3‐methylglutaryl synthases (HMGSs)—TaC and TaF—could constitute two functional ACP‐HMGS pairs (TaB/TaC and TaE/TaF) responsible for the incorporation of acetate and propionate units into the myxovirescin A scaffold, leading to the formation of β‐methyl and β‐ethyl groups, respectively. It has been suggested that three more proteins—TaX and TaY, which are members of the superfamily of enoyl‐CoA hydratases (ECHs), and a variant ketosynthase (KS) TaK—are shared between two ACP‐HMGS pairs, to give the complete set of enzymes required to perform the β‐alkylations. The β‐methyl branch is presumably further hydroxylated (by TaH) and methylated to produce the methoxymethyl group observed in myxovirescin A. To substantiate this hypothesis, a series of gene‐deletion mutants were created, and the effects of these mutations on myxovirescin production were examined. As predicted, ΔtaB and ΔtaE ACP mutants revealed similar phenotypes to their associated HMGS mutants ΔtaC and ΔtaF, respectively, thus providing direct evidence for the role of TaE/TaF in the formation of the β‐ethyl branch and implying a role for TaB/TaC in the formation of the β‐methyl group. Production of myxovirescin A was dramatically reduced in a ΔtaK mutant and abolished in both the ΔtaX and the ΔtaY mutant backgrounds. Analysis of a ΔtaH mutant confirmed the role of the cytochrome P450 TaH in hydroxylation of the β‐methyl group. Taken together, these experiments support a model in which the discrete ACPs TaB and TaE are compatible only with their associated HMGSs TaC and TaF, respectively, and function in a substrate‐specific manner. Both TaB and TaC are essential for myxovirescin production, and the TaB/TaC pair can rescue antibiotic production in the absence of either TaE or TaF. Finally, the reduced level of myxovirescin production in the ΔtaE mutant, relative to the ΔtaF strain, suggests an additional function of the TaE ACP.
Export aspiration system in patients with acute coronary syndrome and visible thrombus provides no substantial benefit
Authors: v. Korn Hubertus , Ohlow Marc , Donev Stoycho , Yu Jiangtao , Huegl Burkhard , Wagner Andreas , Lauer Bernward 
Published: June 2007

AbstractObjective:A visible thrombus remains a risk factor during percutaneous coronary intervention (PCI).Methods:Between October 2004 and March 2005, we treated all patients who consecutively presented with acute coronary syndrome + angiographically visible thrombus using the Export Aspiration System (group 1, n = 79). We retrospectively analyzed all consecutive patients who had been treated without a thrombectomy device (group 2, n = 79).Results:Final TIMI flow 3 or myocardial blush grade (MBG) 3 after completing PCI in group 1 and 2 was seen in 54 patients (68.4%) versus 57 patients (72.2%, P = 0.6) and in 45 patients (57.0%) versus 44 patients (55.7%, P = 0.9), respectively. Final TIMI flow 0 or MBG 0 in group 1 and 2 was observed in eight patients (10.1%) versus four patients (5.1%, P = 0.23) and in 24 patients (30.4%) versus 16 patients (20.3%, P = 0.15), respectively.Histologic evaluation:Histologic evaluation revealed mostly mixed thrombotic material (83.6%). Microscopic material was seen in 91.8% of cases.MACE:Total MACE rate during hospital stay was 11.4% in both groups.Conclusion:Using the Export Aspiration System in patients presenting with acute coronary syndrome + visible thrombus provides no substantial benefit. © 2007 Wiley‐Liss, Inc.
Imaging of vulnerable coronary artery plaques
Authors: Hamdan Ashraf , Assali Abid , Fuchs Shmuel , Battler Alexander , Kornowski Ran 
Published: June 2007

AbstractAdvances in the identification of vulnerable plaque can be an important step in preventing myocardial infarction and sudden cardiac death. The recognition that non‐flow‐limiting plaques often produce cardiac events has led to the development of invasive and non‐invasive methods to identify such plaques prospectively. This review will present the use of noninvasive imaging modalities for identifying vulnerable plaque such as computed tomography and magnetic resonance imaging. We will also review the different invasive modalities such as intravascular magnetic resonance imaging, intravascular ultrasound, coronary angioscopy, coronary thermography, optical coherence tomography, near‐infrared spectroscopy, and palpography. © 2007 Wiley‐Liss, Inc.
Modified T‐stenting with intentional protrusion of the side‐branch stent within the main vessel stent to ensure ostial coverage and facilitate final kissing balloon: The T‐stenting and small protrusion technique (TAP‐stenting). Report of bench testing and first clinical Italian‐Korean two‐centre experience
Authors: Burzotta Francesco , Gwon Hyeon‐Cheol , Hahn Joo‐Yong , Romagnoli Enrico , Choi Jin‐Ho , Trani Carlo , Colombo Antonio 
Published: June 2007

AbstractObjectives:To describe a novel modification of the T‐stenting technique and to report the bench test as well as the first clinical results obtained.Background:The best technique to treat bifurcated coronary lesions has not been defined.Methods:This novel modification of the T‐stenting technique is based, after stenting of the main vessel (MV) and kissing balloon, on the intentional minimal protrusion of the side‐branch (SB) stent within the MV. Final kissing balloon is performed using the balloon kept uninflated into the MV before SB stenting. The technique was tested in vitro and applied in two independent series of patients undergoing elective drug‐eluting stent implantation on one bifurcated lesion. Bifurcated lesions were classified according to the Medina classification. Patients' outcome up to 9 month was prospectively assessed.Results:The bench test showed perfect coverage of the bifurcation with minimal stent's struts overlap at the proximal part of SB ostium and a small, single layer stent struts, neo‐carina not affecting the MV patency. Seventy‐three complex patients (67% of Medina 1,1,1 lesions; 44% of unprotected distal left main lesions) were treated with sirolimus‐, paclitaxel‐, or zotarolimus‐eluting stents using the TAP technique. Procedural success was achieved in all cases and the clinical outcome up to 9 month was characterized by a low rate of clinically‐driven target vessel revascularization (6.8%).Conclusions:The TAP‐stenting is a modification of the T‐stenting technique which allows full coverage of bifurcated lesions and facilitates final kissing balloon. The first clinical experience suggests that this technique may be practical, thus calling for further evaluations of the technique. © 2007 Wiley‐Liss, Inc.
Let's call it “color‐gamut rendering”
Authors: Berns Roy S. 
Published: June 2007

AbstractThe term “color gamut” historically has been associated with color output such as optimal color stimuli and additive and subtractive imaging systems. Recently, this term has been used with input devices such as scanners and digital cameras. It is proposed that the term “color‐gamut rendering” should be used instead of input devices. This clarifies the distinction between input (analysis) and output (synthesis) color systems in terms of the effect of an input system on defining the colorimetric properties of an output system. © 2007 Wiley Periodicals, Inc. Col Res Appl, 32, 334–335, 2007