bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced high‐energy phosphate levels in the painful muscles of patients with primary fibromyalgia
Authors: Bengtsson Ann , Henriksson K. G. , Larsson Jörgen 
Published: February 2007

AbstractMuscle energy metabolism was studied by chemical analysis of biopsy samples from: 1) trigger points in the trapezius muscle from 15 patients with primary fibromyalgia (PF), 2) nonpainful, anterior tibial muscle from 6 patients with PF, and 3) the trapezius muscle from 8 healthy controls. We found a decrease in the levels of adenosine triphosphate, adenosine diphosphate, and phosphoryl creatine, and an increase in the levels of adenosine monophosphate and creatine, in the trapezius muscles from the patients. These findings support the notion that the pain in patients with PF is of muscular origin.
Scleromyxedema with systemic involvement mimics rheumatic diseases
Authors: Fudman Edward J. , Golbus Joseph , Ike Robert W. 
Published: February 2007

AbstractScleromyxedema is an infiltrative skin disease produced by hyaluronic acid deposition in the dermis. A benign monoclonal gammopathy is usually present. We report 2 patients with scleromyxedema and systemic illnesses. Both patients had muscle weakness, dysphagia, and weight loss in addition to the skin changes. One also had sclerodactyly, telangiectasias, and Raynaud's phenomenon. Scleromyxedema with systemic involvement may mimic rheumatic diseases.
Influence of age, gender, and obesity on salicylate kinetics following single doses of aspirin
Authors: Greenblatt David J. , Abernethy Darrell R. , Boxenbaum Harold G. , Matlis Rita , Ochs Hermann R. , Harmatz Jerold S. , Shader Richard I. 
Published: February 2007

AbstractSalicylate kinetics following single, 650‐mg intravenous and oral doses of aspirin were evaluated in humans in 2 studies. Complete conversion of aspirin to salicylate was assumed. The first study involved 25 young (25–40 years) and 21 elderly (66–89 years) healthy male and female volunteers. Mean salicylate clearance was lower in elderly females compared with that in young females; however, the difference between young men and elderly men was not significant. Salicylate free fraction in plasma increased significantly with age in men and women. After correction for free fraction, unbound mean clearance was reduced in elderly men compared with young men, and in elderly women compared with young women. Peak plasma salicylate concentrations after taking oral aspirin were not significantly influenced by age, and systemic availability of salicylate in all groups was complete. The second study compared 20 obese subjects (mean weight 113 kg) with 20 normal weight controls (mean weight 67 kg) matched for age, sex, height, and smoking habits. Small differences between obese and control groups were observed in total salicylate volume of distribution (Vd), unbound Vd, and mean clearance of total or unbound salicylate. Following normalization for total weight, however, values of total Vd and mean clearance were significantly smaller in obese subjects than in normal weight subjects. Rate and completeness of salicylate absorption were not influenced by obesity when aspirin was ingested, although peak levels were lower in obese subjects. If applied to multiple doses, the reduced unbound clearance of salicylate in the elderly would imply increased accumulation unless doses are appropriately adjusted downward. During long‐term therapy, salicylate dosage for obese individuals should not be adjusted upward in proportion to total weight.
The contribution of pain to disability in experimentally induced arthritis
Authors: Dardick Samuel J. , Basbaum Allan I. , Levine Jon D. 
Published: February 2007

AbstractWe examined the differential effects of disease severity and pain on morbidity (as measured by weight loss and decrease in activity) in rats with adjuvant‐induced arthritis. We found that eliminating nociceptive messages from affected extremities, although not significantly affecting the course of the disease, attenuated the morbidity observed in arthritic rats. When pain was factored out, the severity of the arthritis had no significant effect on the same measures of morbidity. These findings suggest that treatment of pain may reduce morbidity, and thereby may have significant benefits beyond that provided by slowing of the disease process.
Lipid microspherule–associated acute monarticular arthritis
Authors: Trostle David C. , Schumacher H. Ralph , Medsger Thomas A. , Kapoor Wishwa N. 
Published: February 2007

AbstractWe describe the fifth reported case of acute monarthritis associated with lipid microspherules. A 33‐year‐old obese man developed acute knee arthritis with a synovial fluid white blood cell count of 11,700/mm3 (97% polymorphonuclear leukocytes). The fluid contained numerous strongly positively birefringent intra‐ and extracellular spherules that measured 2–6 μm in diameter and had the appearance of Maltese crosses. No systemic lipid disorder or local trauma could account for the attack, which responded promptly to the administration of colchicine.
Molecular forms of IgA rheumatoid factor in serum and synovial fluid of patients with rheumatoid arthritis
Authors: Schrohenloher Ralph E. , Koopman William J. , Alarcón Graciela S. 
Published: February 2007

AbstractThe distribution of molecular forms of serum IgA rheumatoid factor (IgA‐RF) in 42 patients with rheumatoid arthritis was examined by solid‐phase radioim‐munoassay following fractionation by gel chromatography or ultracentrifugation in acidic buffer. Analysis of the fractions using phosphate buffered saline indicated that the IgA‐RF in each serum was mainly polymeric. However, monomeric IgA‐RF was detected in sera from approximately two‐thirds of the patients, after dilution of chromatographic or ultracentrifugal fractions in diluent containing mouse monoclonal anti‐human α chain antibody. The levels of monomeric IgA‐RF (mean ± SD 38 ± 86 μg/ml) and the ratios of monomeric to polymeric IgA‐RF (mean ± SD 0.29 ± 0.41) varied over a wide range. Paired synovial fluids from 9 of the patients were also examined. Monomeric IgA‐RF was detected in each, although 2 samples demonstrated only minimal quantities. Neither form of IgA‐RF was detected in serum from healthy adults when analyzed under the same conditions. Thus, both monomeric and polymeric IgA‐RF can occur in serum and synovial fluid from patients with rheumatoid arthritis, and their proportions vary widely among patients.
Metabolism of a cartilage matrix glycoprotein in normal and osteoarthritic canine articular cartilage
Authors: Fife Rose S. , Palmoski Marshall J. , Brandt Kenneth D. 
Published: February 2007

AbstractWe have recently described a 550,000‐dalton noncollagenous cartilage matrix glycoprotein (CMGP), with subunits of 130,000, which is present in hyaline cartilage and fibrocartilage. Biosynthetic studies indicated that CMGP was synthesized by short‐term organ cultures of normal canine articular cartilage, representing approximately 9% of the total 3H‐leucine incorporated into protein in 24‐hour cultures. There was no incorporation of 35S‐sulfate or 3H‐mannose into CMGP under these conditions, but the protein did incorporate 32P‐phosphate. The majority of the 3H‐leucine‐labeled CMGP was removed after 24 hours of chase with unlabeled leucine, and only a small amount remained at 72 hours, which suggests that there was rapid metabolism of the protein. CMGP was not detected in cartilage after addition of cycloheximide to the culture medium; this confirms its short half‐life. Cultures of osteoarthritic cartilage obtained from dogs 8–10 weeks after anterior cruciate ligament transection revealed no difference in the metabolism of CMGP in this tissue compared with that found in cultures of normal articular cartilage.
Arthropathy and cutaneous calcinosis in hemodialysis oxalosis
Authors: Reginato Antonio J. , Seoane Jose Luis Ferreiro , Alvarez Ceferino Barbazan , Piferrer Jorge Mitja , Meijon Lino Vidal , Turon Raul Pascual , Vasconez Fernando , Rivera Exequel R. , Clayburne Gilda , Rothfuss Susan 
Published: February 2007

AbstractRecently, calcium oxalate crystals have been identified in the synovial fluid of patients with arthritis and end‐stage renal failure. We describe 4 patients who, during the course of long‐term hemodialysis, developed calcium oxalate crystal deposits in the synovium and skin. Clinical manifestations included podagra, teno‐synovitis, olecranon bursitis, and acute and chronic synovitis of the large joints that were associated with chondrocalcinosis or subchondral bone erosions. Diffuse involvement of the hand, with chondrocalcinosis of the finger joints, miliary calcified deposits in the skin, and artery calcifications, was observed in 3 patients. The fourth patient had erosive arthropathy. Oxalosis secondary to end‐stage renal failure in patients treated with long‐term hemodialysis can present with articular manifestations that resemble those of gout, pseudogout, and apatite deposition disease. Other characteristic features of the synovitis associated with oxalosis secondary to end‐stage renal disease were: predominant involvement of the hand, mild inflammatory changes in the synovial fluid and synovium, and poor response to administration of nonsteroidal antiinflammatory agents.
Monosodium urate crystals in the knee joints of patients with asymptomatic nontophaceous gout
Authors: Bomalaski John S. , Lluberas Geronimo , Schumacher H. Ralph 
Published: February 2007

AbstractWe aspirated synovial fluid from the knees of 50 patients with asymptomatic, nontophaceous gout, in whom synovial fluid monosodium urate (MSU) crystals had previously been documented in the knees or other joints. Fifty‐eight percent of these asymptomatic patients had MSU crystals in their knee joints. Serum uric acid levels, serum creatinine levels, volume of synovial fluid aspirated, and cell counts of the aspirated fluid did not differentiate the MSU crystal‐positive group from the group without MSU crystals. Clinical factors such as alcohol abuse, coronary heart disease, hypertension, duration of gout, duration of the intercritical period, and drug therapy did not differentiate the 2 groups. Nineteen patients consented to aspiration of their other knee. Seven of these patients (37%) had MSU crystals bilaterally, and 6 patients (32%) had them unilaterally. The implications of the persistence of MSU crystals (including those in intracellular locations) in many patients, despite normalization of serum uric acid levels, should be determined. Knee joint aspiration is a sensitive method for the demonstration of MSU crystals in asymptomatic patients. The procedure might also be useful in documenting these crystals in patients who have had attacks of arthritis with features consistent with a diagnosis of gout, but in whom MSU crystals have not been documented.
Multiple unusual manifestations of Wegener's granulomatosis: Breast mass, microangiopathic hemolytic anemia, consumptive coagulopathy, and low erythrocyte sedimentation rate
Authors: Jordan Joanne M. , Manning Michael , Allen Nancy Bates 
Published: February 2007

AbstractWe describe a patient who presented with multiple unusual manifestations of Wegener's granulomatosis, including a breast mass, microangiopathic hemolytic anemia, and consumptive coagulopathy. Erythrocyte sedimentation rate (Westergren), usually elevated in the course of this disease, persistently remained low in this patient. We found that C‐reactive protein levels were, in this case, a more sensitive gauge of disease activity.
Changes in cell surface antigen expression on human articular chondrocytes induced by gamma‐interferon
Authors: Jahn Beatrice , Burmester Gerd R. , Schmid Hansjörg , Weseloh Gerd , Rohwer Peter , Kalden Joachim R. 
Published: February 2007

AbstractIa antigens (class II HLA molecules) have been detected on cells eluted from affected human cartilage in certain disease states, but not on normal cartilage cells. Because the presence of Ia antigens on chondrocytes may play an important role in rheumatic diseases, we investigated the induction of these molecules by γ‐interferon (γ‐IFN), a potent Ia‐inducing lymphokine. Human articular chondrocytes were incubated with recombinant γ‐IFN, and the expression of Ia antigens was studied by cell sorter analysis, using a panel of reagents that detect monomorphic and polymorphic specificities of the DR and DQ Ia antigen families. While the induction of DR antigens, including polymorphic DR specificities, was readily obtained with γ‐IFN (50–95% positive cells), DQ antigens were negative or were displayed only on a lower percentage of chondrocytes (5–60%). In addition, incubation with γ‐IFN led to an increased expression of HLA class I antigens. The expression of various other surface markers either remained unchanged (as in 4F2 and BA‐2) or showed tendencies toward decreased percentages (as in 83c2) or increased percentages (as in MøR‐17). No apparent change in cell morphology or growth pattern was observed.
An Open, Controlled, Randomized Comparison of Cyclosporine and Azathioprine in the Treatment of Rheumatoid Arthritis: A Preliminary Report
Authors: FØRre ØYstein , Bjerkhoel Frithjof , Salvesen Christian F. , Berg Knut J. , Rugstad Hans E. , Saelid Gunnar , Mellbye Ove J. , KÅSs Erik 
Published: February 2007

AbstractCyclosporine (10 mg/kg/day) and azathioprine (2.5–3 mg/kg/day) were compared for 26 weeks in an open, controlled, randomized study of 24 patients with rheumatoid arthritis. Each treatment group consisted of 12 patients. Those patients who took cyclosporine improved significantly in the 50‐foot walk time, circumferences of proximal interphalangeal joints, Ritchie articular index, global assessment by investigator, and grip strength, when compared with baseline findings. In the azathioprine group, there was improvement only in grip strength.
Cytomegalovirus Retinitis in Rheumatic Disease: A Case Report
Authors: Winkler Anne , Finan Michael J. , Pressly Thomas , Roberts Randy 
Published: February 2007

AbstractWe describe a 67‐year‐old white man with Wegener's granulomatosis who was treated with cyclophosphamide, then with azathioprine, and who subsequently developed cytomegalovirus retinitis. The patient initially improved on a course of adenine arabinosine, but his retinitis has progressed, despite discontinuation of immunosuppressive therapy. We show that cytomegalovirus retinitis does occur in patients with rheumatic diseases who receive immunosuppressive therapy.
Subset specificity of antilymphocyte antibodies in systemic lupus erythematosus
Authors: Winfield John B. , Shaw Melody , Yamada Akira , Minota Seiji 
Published: February 2007

AbstractUsing indirect immunofluorescence and flow cytometry, we determined the proportion and number of T3+, T4+, and T8+ cells in the peripheral blood of patients with systemic lupus erythematosus whose sera were positive for cold‐reactive antilymphocyte antibodies versus values in patients whose sera were negative for these antibodies. There was a disproportionate reduction in T4+ peripheral lymphocytes when cold‐reactive antilymphocyte antibodies preferentially cytotoxic for this subpopulation were present in autologous serum. The decrease in this subset was responsible for a reduction in the T4:T8 ratio; variation in the number and proportion of T8+ cells was insignificant. A similar, but autoantibody‐independent, alteration in the T4+ subpopulation was found in patients who were receiving prednisone therapy. A relationship between T cell population abnormalities and systemic lupus erythematosus disease activity, per se, was not observed.
The interaction of antibody/dna immune complexes with complement
Authors: Taylor Ronald P. , Edberg Jeffrey C. , Kujala Gregory A. , Sloman Andrew J. , Wilson Cara C. , Cronin Mary E. 
Published: February 2007

AbstractIn 28 serum and plasma samples from patients with systemic lupus erythematosus, we examined the importance of antibody class with respect to complement‐mediated binding to human red blood cells (RBC) of antibody/DNA immune complexes (IC) prepared with anti‐DNA antibodies. We used both 3H–double‐stranded DNA and 3H–single‐stranded DNA (ssDNA). Generally, double‐stranded DNA IC showed considerably higher binding than did ssDNA IC in the RBC binding assay. Further analysis indicated that although ssDNA IC fix complement, it is necessary that these IC contain IgM anti‐DNA antibodies in order for them to bind to RBC. The results suggest that the mechanisms of clearance and pathogenic potential of these IC may depend upon both the DNA conformation and antibody class. In particular, complement‐fixing IC which contain IgG anti‐DNA antibodies and ssDNA may not be cleared via the erythrocyte clearance mechanism, and therefore, could be more likely to deposit in certain tissues and initiate inflammatory reactions.
Hereditary amyloidosis: description of a new american kindred with late onset cardiomyopathy
Authors: Benson Merrill D. , Wallace Margaret R. , Tejada Eduardo , Baumann Hal , Page Burnestine 
Published: February 2007

AbstractA family with hereditary amyloidosis characterized by peripheral neuropathy and cardiomyopathy is described. Lack of eye involvement sets their disease apart from the Indiana/Swiss familial amyloidotic polyneuropathy type II. The disease is of late onset; affected members die of cardiomyopathy after age 60. The late onset and lack of clinically significant neuropathy in several family members has led to misdiagnosis of the cardiomyopathy. Immunohistochemistry using antiprealbumin antiserum showed staining of amyloid deposits in nerve and heart.
Creatine kinase activity inhibitor in sera from patients with muscle disease
Authors: Kagen Lawrence J. , Aram Sirarpi 
Published: February 2007

AbstractSera from patients with muscle disease contain an inhibitor of creatine kinase (CK) which may lead to underestimation of CK activity. The inhibitor was dialyzable, but was not related to previously described low molecular weight inhibitors of CK. In a small group of patients with myositis, the amount of inhibitor present in serum was correlated with the severity of the illness. Our findings suggest that the CK inhibitor may be released into serum from injured muscle.
Immunity to soluble retinal antigen in patients with uveitis accompanying juvenile rheumatoid arthritis
Authors: Petty R. E. , Hunt D. W. C. , Rollins D. F. , Schroeder M.‐L. , Puterman M. L. 
Published: February 2007

AbstractStudies of immunity to bovine soluble retinal antigen (antigen S) were carried out using serum and peripheral blood lymphocytes from children with juvenile rheumatoid arthritis and chronic anterior uveitis (JRA‐uveitis), children with JRA alone, children with nonrheumatic diseases, and controls who had no ocular or rheumatic disease. Enzyme‐linked immunosorbent assay and the lymphocyte transformation assay were used to determine immunity. Antibody to antigen S was present significantly more frequently in children with JRA‐uveitis than in children with JRA alone, children with nonrheumatic disorders, or controls. These latter groups did not differ in positivity for this antibody. Lymphocyte transformation occurred more frequently in children with JRA–uveitis than in children with JRA alone or controls. Children with JRA alone and controls had similar frequencies of lymphocyte transformation positivity. Enzyme‐linked immunosorbent assay positivity and lymphocyte transformation positivity tended to occur in different children. Children with JRA–uveitis who had HLA–B35 had the highest frequency of antibody to antigen S. Immunity to antigen S may be the result of ocular damage by mechanisms other than a pathogenic mechanism per se.
Failure Of klebsiella pneumoniae antibodies to cross‐react with peripheral blood mononuclear cells from patients with ankylosing spondylitis
Authors: Cameron Fiona H. , Russell Pamela J. , Easter Joan F. , Wakefield Denis , March Lyn 
Published: February 2007

AbstractCross‐reactivity between antibodies to 2 strains of Klebsiella pneumoniae (K43 and F77) and the peripheral blood lymphocytes of patients with ankylosing spondylitis (AS) was examined in 3 separate antibody binding and cytotoxicity assays. Using K pneumoniae antisera in a chromium release cytotoxicity assay, we found no difference in the reactions of cells from AS patients and those from control subjects. This result contrasts with the results of previous studies. Similarly, using an enzyme‐linked immunosorbent assay, we detected no significant increase in antibody binding to peripheral blood mononuclear cells (PBMC) in HLA–B27 positive patients with AS. Low levels of antibody binding were detected by a fluoresceinated antibody binding assay; however, normal rabbit serum, which was used as a control, was shown to have a binding affinity for PBMC that was significantly greater than that of specific K pneumoniae antisera. The results of our present study do not support the concept of a specific cross‐reactivity between antibodies to K pneumoniae and the PBMC of patients with AS who are HLA–B27 positive.
Intravenous pulse methylprednisolone treatment of juvenile dermatomyositis
Authors: Laxer Ronald M. , Stein Leonard D. , Petty Ross E. 
Published: February 2007

AbstractRecent observations that juvenile dermatomyositis (JDMS) may be a heterogenous disorder, in both its clinical course and prognosis, suggest that subgroups of patients with this disease may respond to various modes of therapy. Therefore, current treatment recommendations of daily, long‐term oral corticosteroid treatment, with its attendant side effects, may not be necessary for all children with JDMS. We report the results of treatment with high‐dose, pulse intravenous methylprednisolone in 7 children with JDMS, 3 of whom had an excellent response with complete remission and no need for daily steroid therapy. We suggest that patients with early, mild JDMS may be candidates for therapy with intravenous methylprednisolone.
Transient fall in serum salicylate levels following intraarticular injection of steroid in patients with rheumatoid arthritis
Authors: Baer Philip A. , Shore Abraham , Ikeman Robert L. 
Published: February 2007

AbstractOur study of 13 hospitalized patients with rheumatoid arthritis examined the effect of intraarticular injection of steroid on serum salicylate levels. A mean decrease in serum salicylate levels of 42 ± 14.5% was observed in 12 patients within 12–36 hours post‐injection (P <0.005). This interaction, though transient, may be very significant clinically. It may lead to erroneous conclusions concerning patient compliance, unwarranted increases in salicylate dosage and resultant toxicity, and delay of appropriate therapeutic maneuvers.
Synovial fluid analysis for diagnosis of amyloid arthropathy
Authors: Lakhanpal Sharad , Li Chin Y. , Gertz Morie A. , Kyle Robert A. , Hunder Gene G. 
Published: February 2007

AbstractTo detect the presence of amyloid, we studied synovial fluid samples from 7 patients who had biopsyproven amyloidosis and clinical arthropathy and from 98 patients who had other forms of arthritis. Centrifuged sediments from synovial fluids of all 7 patients with amyloid and clinical arthropathy were positive on staining with Congo red, whereas the 98 others were negative. Under polarized microscopy, the amyloid appeared as an amorphous material with strong Congo red uptake and typical apple‐green birefringence. These findings indicate that analysis of synovial fluid sediments with Congo red may represent a simple, yet sensitive, test for the diagnosis of amyloid arthropathy.