bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical diagnostic value of circ-ARHGER28 for breast cancer and its effect on MCF-7 cell proliferation and apoptosis
Authors: Tao Xiang , Na Li , Hu En-xi , Wang Jia , Wu Li-gang , Zhang Xu , Wang Li-bin 
Published: March 2024

Abstract

Objective
To investigate the clinical diagnostic value of circ-ARHGER28 in breast cancer (BC), and the biological functiona of circ-ARHGER28 on the proliferation and apoptosis of MCF-7 cells.
Methods
Human circRNA microarray was performed to analysis the expression of circRNAs in BC patients. RT-qPCR combined with bioinformatic analysis was applied to verify the candidate circRNAs in BC tissues and peripheral blood. Circ-ARHGER28 was chosen as the candidate gene for further research. The clinical diagnostic value and biological functions of circ-ARHGER28 were analyzed. The over-expression and inhibit vector of circ-ARHGER28 were constructed and transfected to MCF-7 cells, the CCK-8 assay and clone formation experiments were applied to detect the cells proliferative and migratory abilities. Flow cytometry analyzed cell apoptosis and cell cycle distribution. RTqPCR and western blot were performed to detect the expression of apoptosis and PI3K/AKT/mTOR associated genes and proteins.
Results
Over-expression of circ-ARHGER28 inhibited the proliferation, colony formation and migration of the MCF-7 cells, as well as increased the population of the cells G2/M phase and apoptotic rate. The apoptosis associated genes and proteins were significantly increased, whereas the gene and protein of PI3K, AKT and mTOR were decreased in the cells.
Conclusion
circ-ARHGER28 has a good correlation with BC. Circ-ARHGER28 inhibited MCF-7 cells proliferation and increased its apoptotic rate. The function of circ-ARHGER28 was associated with the PI3K/AKT/mTOR signaling pathway. Circ-ARHGER28 proved to be an ideal biomarker for BC diagnostic and a novel target for BC therapy.
A hybrid multi-strategy SCSO algorithm for robot path planning
Authors: Lou Tai-shan , Yue Zhe-peng , Chen Zhi-wu , Qi Ren-long , Li Guang 
Published: March 2024

Abstract

To address the problems of low convergence efficiency and easy to fall into local extremes in the sand cat swarm optimization algorithm for solving the path planning problem of mobile robots. A hybrid multi-strategy based sand cat swarm optimization (HMSCSO) algorithm is proposed to solve the path planning problem of mobile robots. First, the nonlinear adjustment strategy is used to increase the convergence accuracy of the algorithm; Then, the logarithmic weight strategy is introduced into the position update to balance the exploration and exploitation ability of the algorithm; Next, the alternate selection strategy is used to improve the ability of the algorithm to jump out of local extremes; Finally, the position update formula of Lévy flight is introduced into the algorithm to alleviate the situation that the algorithm falls into stagnation. To verify the effectiveness of the proposed HMSCSO algorithm, 23 benchmark test functions and CEC2022 test functions are selected for comparison with other advanced optimizers. In addition, the HMSCSO algorithm is subjected to ablation experiments in three groups of environments with different obstacles. The experimental results show that the HMSCSO algorithm can effectively improve the solution accuracy.
Rich 3D Experience Digital Gallery for Heritage Museums –Enhancement Approach
Authors: K Akila 
Published: March 2024

Abstract
With the massive progression of information technologies, the digitization method has emanated to be more advanced and diversified. Presently, a few well-known museums have created virtual environments in the form of digitized information on their web pages or pre-recording their visit escort through portable devices, which provides faster and at ease access without any time and space limitation and makes the visitors more convenient. Thus, the versatile analysis of virtual museums has the likely to facilitate a better recognize of the values of resources to depot cultural heritage. To bridge a gap between visitors and monuments, we have to reinforce the importance of interactive activities at a greater level of engagement and sense of presence. It could be achieved through the implementation of Augmented Reality. Fortunately, the expectation of museum exhibitions is enhanced by blending the visit experience with more appropriate interaction with real objects and embodiment of personalization. By fusing emotionally engaging stories, a remarkable blend of historical artifacts, characters, activities to offer a fair experience while the cultural site is remote. Our intention is to describe various approaches that aid to boost a visitor’s virtual experience in a museum and art galleries over digital technology. Our work focuses on improvising the virtual medium to offer effective engagement for visitors with multiple delights in museums and art galleries using advanced technologies.
Enhancing Stroke Rehabilitation with Whole-Hand Haptic Rendering: Development and Clinical Usability Evaluation of a Novel Upper-Limb Rehabilitation Device
Authors: Rätz Raphael , Conti François , Thaler Irène , Müri René M. , Marchal-Crespo Laura 
Published: March 2024

Abstract

Introduction: There is currently a lack of easy-to-use and effective robotic devices for upper-limb rehabilitation after stroke. Importantly, most current systems lack the provision of somatosensory information that is congruent with the virtual training task. This paper introduces a novel haptic robotic system designed for upper-limb rehabilitation, focusing on enhancing sensorimotor rehabilitation through comprehensive haptic rendering.
Methods: We developed a novel haptic rehabilitation device with a unique combination of degrees of freedom that allows the virtual training of functional reach and grasp tasks. Thereby, we use a physics engine-based haptic rendering method to render whole-hand interactions between the patients' hands and virtual tangible objects. To evaluate the feasibility of our system, we performed a clinical mixed-method usability study with seven patients and seven therapists working in neurorehabilitation. We employed standardized questionnaires to gather quantitative data and performed semi-structured interviews with all participants to gain qualitative insights into the perceived usability and usefulness of our technological solution.
Results: The device demonstrated ease of use and adaptability to various hand sizes without extensive setup. Therapists and patients reported high satisfaction levels, with the system facilitating engaging and meaningful rehabilitation exercises. Participants provided notably positive feedback, particularly emphasizing the system's available degrees of freedom and its haptic rendering capabilities. Therapists expressed confidence in the transferability of sensorimotor skills learned with our system to activities of daily living, although further investigation is needed to confirm this.
Conclusion: The novel haptic robotic system effectively supports upper-limb rehabilitation post-stroke, offering high-fidelity haptic feedback and engaging training tasks. Its clinical usability, combined with positive feedback from both therapists and patients, underscores its potential to enhance robotic neurorehabilitation.
Genetic Diversity Analysis in Sorghum [Sorghum bicolor (L.)] by Using SSR Markers
Authors: Kale Sonam S. , Borde Gaurav , Ansari Mushtaq Ahmad , Ahmad Sheikh Fayaz , Malik Abdul , Shahid Mudassar 
Published: March 2024

Abstract

Sorghum bicolor is one of the most important cereal crops around the world where the availability of water is limited. The study of genetic variability among the sorghum accessions will enable accurate results in breeding programs and in mapping studies. Using SSR markers that were spread out evenly on the sorghum genome, it was found that all 23 accessions from the Maharashtra region could be put into three main classes. These classes were linked to the biological status and other traits and characteristics of the accessions. The gene diversity measured in terms of polymorphic information content (PIC) ranged from 0.00 to 0.24, with the average PIC value found to be 0.067, where a 0.24 PIC value indicated the presence of two alleles per locus. Expected heterozygosity, or gene diversity, was computed according to Nei (1973) and varied from 0.00 to 0.28 with an average of 0.07. A total of 9 SSR marker alleles were detected, with an average of 1.5 alleles per marker. The number of alleles per marker ranged from 1 (msbCIR329, Xtxp021, and Xtxp278) to 2 (Xtxp321, msbCIR306, and Xisep0310). The fragment size of the allele ranged from 109 (msbCIR329) to 252 (Xtxp321 and Xtxp278).
The Metabolic Score for Insulin Resistance (METS-IR) as a Predictor of Incident diabetes: A Longitudinal Study among Chinese without Diabetes
Authors: Lin Yajuan , Liu Zexin , Li Ziwen , Li Jiatian , Bao Xiaoyan , Xia Yunlong , Zhang Bo 
Published: March 2024

Abstract

Background: As the prevalence of diabetes rises and the disease burden increases, it will be extremely beneficial to identify high-risk groups who are susceptible to diabetes early on using simple and effective diagnostic tools. Recently, it was discovered that T2DM is strongly correlated with METS-IR. However, it is unclear whether the relationship between T2DM and METS-IR is affected by time progression.
Methods
This study included 114,827 subjects (62186 men and 52641 women) who underwent a physical examination with a 7-years follow-up. The subjects were divided into five equal groups based on their METS-IR quintile, with the outcome of interest being the occurrence of diabetic events.
Results
Multivariate Cox proportional hazards regression analysis for the prediction of diabetes according to the METS-IR shows that METS-IR was strongly positively correlated with the risk of future diabetes (HR 1.11 per SD increase, 95% CI: 1.10-1.11), and the risk of diabetes corresponding to METS-IR was gradually increased compared with the lowest quintile group (P for trend < 0.001). ROC curve analysis demonstrated that METS-IR in females outperformed the prediction for T2DM compared to that in males. Among people with different phenotypes, it can be observed that younger individuals had a significantly higher risk of METS-IR-related diabetes than middle-aged and older individuals.
Conclusions
In summary, the present study demonstrated that METS-IR is an important independent predictor of new-onset diabetes in the Chinese general population, independent of traditional diabetes risk factors, especially in younger individuals.
Growth evaluation in Silver Russel Syndrome and Small Gestational Age patients : A Statistical Model
Authors: Migliaretti Giuseppe , Stura I. , Brondino F. , Matarazzo P. , Sanctis L. 
Published: March 2024

Abstract

Objective
The study proposes a description and a mathematical model of growth of both Silver Russell Syndrome (SRS) and Small for Gestational Age (SGA) patients, with and without Growth Hormone (GH) treatment.
Design and Methods
Thirteen patients with molecular diagnosis of SRS were compared with 13 sex-paired SGA subjects. For each patient, neonatal and follow up auxological data were collected. A predictive growth model based on the Gompertzian function and optimization method, already tested and validated on a cohort of GH-deficient children, was used.
Results
Baselines anthropometric values do not significantly differ between the presented SRS and SGA populations. The applied mathematical model showed same natural growth hope for SGA and SRS subjects. In SRS patients under GH treatment a significant increase in growth hope seems to emerge from the prediction model, while an improvement in growth velocity was assessed in the counterpart of SGA subjects, which exhibit early response to the treatment.
Conclusions
The proposed mathematical prediction model indicates a benefit from GH treatment for both the SRS and SGA conditions; while SGA patients showed an earlier response, SRS patients have a longer term gain. These data suggest the need to anticipate the SRS diagnosis for starting with earlier therapy.
Impact of aluminium doping on structural, magnetic and dielectric properties of magnetite nanoparticles
Authors: Donta Paramesh 
Published: March 2024

Abstract
Aluminium substituted nanoferrites Fe(3−x)AlxO4 with (x = 0.0, 0.1, 0.5, 1.0) samples were routed through auto-combustion. X-ray diffraction established the spinel cubic structure with no marking of impurities and the lattice constant were in between 8.398 Å to 8.359 Å. Crystallite size specifies the nano size formation and it varies from 32.15 nm to 21.38 nm with aluminium substitution. The field emission scanning electron microscope images shown the particles are in nano sized with uniform distribution. The energy dispersive X-ray analysis established the all basic elemental composition. FTIR spectra remarked two basic characteristic absorption bands, ν1 and ν2 between 600 cm− 1 and 404 cm− 1. VSM studies at room temperature remarked that the saturation magnetization (Ms), coercivity (Hc) and remanent magnetization (Mr) was decreased with aluminium substitution. Impedance spectroscopy studies were analysed with applied frequency and temperature to explore the dielectric nature of samples and evidenced that grain boundaries have more influence on the resistivity than grains. A clear deviation was observed in dielectric loss, dielectric permittivity and AC conductivity with temperature and frequency. This elucidated by the Maxwell-Wagner polarization and supports the hopping of Fe3+ and Fe2+ ions at the B-sites.
The previously undescribed variant of the thyrohyoid muscle and its potential impact on surgical procedures
Authors: Maślanka Krystian , Zielinska Nicol , Karauda Piotr , Węgiel Andrzej , Niemiec Małgorzata , Olewnik Łukasz 
Published: March 2024

Abstract

The thyrohyoid muscle belongs to the infrahyoid group located in the carotid triangle. It normally originates from thyroid cartilage and inserts into hyoid bone. Quite often, it is continuous with the sternohyoid muscle. Furthermore, there are variants that have their origin in the cricoid cartilage only, however, this occurs very rarely. During anatomical dissection, a two-headed variant of this muscle was found. One head had its origin in the cricoid cartilage and the other in the thyroid cartilage. This variant of thyrohyoid had not been previously described in the available literature. Therefore, we believe that it may be referred to as the cricothyrohyoid muscle. As the thyrohyoideus is often used as a landmark during surgical procedures in the prelaryngeal area and as a muscle graft, a thorough knowledge of its anatomy and variation is extremely important. We speculate that the two-headed version of this muscle may be problematic during surgical procedures in this region, however, it may also provide more options as a muscular graft.
Promoting HPV Vaccination Against Cervical Cancer in Low- and Middle-Income Countries: A Framework for Budget Impact Analysis and Case Study in China
Authors: Li Yi , Chai Peipei , Liu Bingjie , Zhou Liangru , Zhang Xin 
Published: March 2024

Abstract
Background
Human Papillomavirus (HPV) vaccination is an effective preventive measure against cervical cancer. Low- and Middle-Income Countries (LMICs) burdened with a high incidence have not implemented National Immunization Programs (NIP) of HPV vaccines. In the context of limited resources, the economic evidence provided by Budget Impact Analysis (BIA) becomes crucial for the inclusion of vaccines in the NIP. Unfortunately, the main issue contributing to insufficient evidence in LMICs may be that LMICs do not have a complete, referenced, and standardized BIA framework for HPV vaccines to conduct their analyses. This study establishes a BIA framework to promote the immunization of HPV vaccine in LMICs and provides a case study in China.
Methods
BIA framework for introducing vaccines into immunization programs was developed following the principles of the International Society for Pharmacoeconomics and Outcomes Research. The case study of China estimated the impact of HPV vaccination on BMIS fund in 2019–2110. It postulated differing reimbursement rates and market substitution rates for imported and domestically produced bivalent HPV vaccines. Parameters were derived from public sources, published literature, and a database of field surveys on treatment costs for patients of cervical cancer in China. Outcome indicators encompassed the incidence of cervical cancer, treatment costs of cervical cancer, vaccination costs, and etc.
Results
The framework consisted of three parts: target population, BIA, and uncertainty analysis, with three scenarios based on vaccine prices and reimbursement rates. In the case of China, without immunization program, the average yearly spent on cervical cancer treatment throughout the country amounts to $6,736,507.32. Upon the execution of the immunization program, this expenditure reduced to $6,645,564.95 each year. Under Scenario 1–3, the BMIS vaccine reimbursement costs were $93,769,903.10, $67,390,961.06, and $88,374,244.91, respectively. During the NIP implementation period, annual vaccination costs range between 0.06 to 0.46 parts per million of current fund expenditure and 0.40 and 2.25 parts per million of current fund balance.
Conclusions
This study has developed a BIA framework for HPV vaccination, aiming to provide a reference for policy formulation in other LMICs with a high burden of cervical cancer that have not yet included the vaccine in their NIPs.
Isoniazid Preventive Therapy Adherence among Soldiers on HIV Antiretroviral Therapy at a General Military Hospital in Uganda
Authors: Moses Sabila , Mupeere Ezekiel , Nangendo Joan , Semitala Fred , Kalyango Joan , Chemonges Saul , Muwanguzi Patience , Katamba Achilles 
Published: March 2024

Abstract
Uganda has a heavy double burden of tuberculosis (TB) and currently ranks among the seven 9 highest TB-affected countries globally. The World Health Organization (WHO) recommends 10 isoniazid preventive therapy (IPT) for treating latent TB. However, the adherence to IPT in 11 military settings has not been well documented in Uganda. 12 We conducted a cross-sectional study among 300 HIV-positive patients receiving 13 antiretroviral therapy (ART) at the General Military Hospital in Uganda. The Consecutive 14 sampling method was used to select clients and a modified Poisson regression model of 15 family Poisson and link log with robust standard errors to adjust for overinflated variances 16 was used for analysis.
Among the 300 clients, the prevalence of IPT was 94.7%, 95% CI: 92.1-97.2; adherence to 18 IPT was associated with being aged ≥ 50 years, with a prevalence ratio (PR) of 1.061 and 19 95% CI: 1.01-1.12; being married, with a PR of 1.438, 95% CI:1.12-1.84; having social 20 support, with a PR of 1.498, 95% CI: 1.17-1.92; and having social support played a significant 21 role in IPT adherence among married participants, with a PR of 0.817, 95% CI: 0.72-0.93. 22 There is a need for strategies to improve access to, retention of and adherence to IPT for 23 young soldiers. Additionally, advocating for social support and behavioural interventions is recognized as a crucial requirement for improving IPT adherence among soldiers living with 25 HIV. More research is needed to explore the role of social support in reducing the social 26 stigma associated with HIV-positive individuals. The findings from this study suggest a 27 necessity to boost IPT adherence among married participants living with HIV, and the model 28 could be applied in other resource-constrained and low-middle-income countries.
Degree of Physical activity during pregnancy and pregnancy related complication
Authors: Hailu Molla , Amare Natnael , Tunta Abayneh , Seyoum Zeru 
Published: March 2024

Abstract

Background: Even though motherhood is a positive and enjoyable experience, it is also a vulnerable period that can be accompanied by various complications. Hypertensive disorder of pregnancy and gestational diabetes mellitus are of the most significant contributors to these complications. Therefore, the main objective of this study is to assess if the of degree of physical activities during pregnancy has impact on the development of hypertensive disorder of pregnancy and gestational diabetes mellitus.
Methods: A cross-sectional study was conducted in 150 women who gave birth from November 2021 to February 2022 at MTUTH, Ethiopia. Data was collected through face-to-face interviews using a structured questionnaire. Descriptive statistics, linear regression, chi-square, and t-test were conducted to check the presence of any association between the dependent and independent variables. A p-value of < 0.05 was considered statistically significant.
Result: Among the total study participant, 65(43.3%) and 85(56.7%) of them were engaged in vigorous physical activities and light physical activities during pregnancy, respectively. From these study participants 62 (41.3%) of them had pregnancy related complication. It was found that women who engaged in vigorous physical activity had a lower proportion of complications (17.74%) compared to those who engaged in light physical activity (82.26%). This difference was statistically significant (p=0.001).
Conclusion: The finding of this study showed that pregnancy related complication, specifically HDP and GDM, was low in women who had vigorous physical activities during pregnancy as compared to those that have sedentary life style or engaged in light physical activities.
Simple imaging system for label-free identification of bacterial pathogens in resource-limited settings
Authors: Clément DOUARRE , Dylan DAVID , Marco FANGAZIO , Emmanuel PICARD , Emmanuel HADJI , Olivier VANDENBERG , Barbara BARBE , Liselotte HARDY , R. MARCOUX Pierre 
Published: March 2024

Abstract

Fast, accurate and affordable bacterial identification methods are paramount for timely treatment of infections, especially in resource-limited settings (RLS). However, today only 1.3% of the sub-Saharan African diagnostic laboratories are performing clinical bacteriology. To improve this, diagnostic tools in RLS should be simple, affordable and maintenance-friendly, in contrast with the expensive machinery used in high-income countries for identification, such as such as Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS). We present a new high-throughput approach based on a simple wide field (864 mm2) lensless imaging system allowing for the acquisition of a large portion of a Petri dish coupled with a supervised deep learning algorithm for identification at the bacterial colony scale. This wide-field imaging system is particularly well suited to RLS since it includes neither moving mechanical parts nor optics. We validated the approach by acquiring a dataset of clinical isolates: 257 isolates from five species among the most frequent pathogens were grown from samples of various types. Resulting optical morphotypes showed variability, including within the same species: a configuration much closer to field clinical settings than focusing only on reference strains. Despite this variability, high identification performance was achieved: the correct identification rate at the species level was 91.7%. These results open up some new prospects for identification in RLS.