bluuebunny/crossref_metadata_2025_split huggingface dataset:
ВПЛИВ РАДИАЛЬНОГО НАТЯГА БОЛТІВ НА ХАРАКТЕРИСТИКИ НДС МОДЕЛЕЙ З'ЄДНАНЬ ЕЛЕМЕНТІВ КОНСТРУКЦІЙ ЛІТАКА
Authors: Yifang Sun 
Published: April 2024

To study the effect of radial bolt tension on the VAT characteristics of the bolted connection elements of aircraft structures, calculations are carried out using the finite element method, which is based on experimental verification using a model of the bolted connection design with tension. The constructed model is verified by the test of bolted connections, based on the reliability of the three-dimensional model of elastoplastic analysis by the finite element method. A model of the analysis of the influence of the design parameters of the bolted connection for six different tension values was created. A comparative analysis of the influence of different tension values on the nature of the VAT distribution, the initial stiffness and the load-bearing capacity of the bolted connection design was carried out. The results of the study showed that the stress distribution and their concentration can be changed when applying the appropriate fit with tension. At a tension value of 1.5 % of the bolt diameter, the initial stiffness and bearing capacity increase by 14.1 % and 9.33 %, respectively.
Administrative and legal regulation of licensing for medical practice
Authors: Batryn О. V. 
Published: April 2024

The article is devoted to the peculiarities of legal regulation of licensing for medical practice. The provisions of laws and regulations governing the procedure for obtaining a licence to practice medicine are studied. The scientific works of scholars in the fields of law, medicine, and public administration who have studied legal relations in the field of healthcare and licensing are studied. Recommendations for amending the legislation are formulated.
The author proposes a definition of licensing for medical practice as a means of state regulation of medical care and medical assistance compliance with the established licensing conditions with a view to protecting the life and health of patients and ensuring the provision of quality medical services.
The article establishes that there are certain peculiarities of licensing for medical practice in comparison with other types of economic activity. A specially created licensing commission of the Ministry of Health of Ukraine reviews documents for obtaining a licence. The licensing regulations set out specific personnel, technological and organisational requirements that the founders of healthcare facilities must meet before submitting documents for a licence. A special package of documents is prepared to be attached to the licence application and provides for the description of the material and technical base and personnel of the healthcare facility. The activities of the entire healthcare facility, rather than individual healthcare professionals, are subject to licensing.
A number of proposals for amendments to the legislation are proposed, namely: 1) to consolidate the concept of medical service as a separate, specific type of medical procedure, diagnosis of a particular disease, rehabilitation, cosmetic care, health massage, paid examination; 2) to define in the licensing conditions more detailed technological requirements for the area of certain premises of a healthcare facility and medical equipment necessary for the treatment of certain diseases; 3) the author supports the position that it is expedient to develop and adopt the Medical Code of Ukraine as a comprehensive legislative act which would regulate legal relations in the field of healthcare.
Countering hostile sabotage and reconnaissance groups by the National Police of Ukraine units in the context of martial law
Authors: Borovyk M. O. 
Published: April 2024

The article is devoted to the study of ways to improve the tactical and special readiness of police officers to counteract sabotage and reconnaissance groups in the border areas and in the areas of active hostilities. The author identifies additional powers of the police under the martial law regime, including the following: conducting joint operations against sabotage and reconnaissance forces of the aggressor (enemy) and paramilitary or armed groups which are not regulated by the laws of Ukraine.
To address the objectives of the study, the analysis of the sources of international and national law providing for the use of sabotage and reconnaissance groups by the parties to the conflict is carried out, the essence and hierarchical structure of these groups are revealed, and the objects against which the use of sabotage measures is prohibited are identified. The article also provides a list of objects of the warring parties which, according to international humanitarian law, may be subject to damage.
The organisational features of the basics for defensive combat by the units of the ground forces of certain countries, in particular, the Federal Republic of Germany and the United States of America, are considered. According to the military doctrine of these countries, the purpose of defence is to disrupt the enemy’s offensive actions, inflict maximum losses in manpower and equipment, and gain time to concentrate forces in the chosen direction for the offensive.
The article describes the signs that can be used to identify members of Russian sabotage and reconnaissance groups. These signs are the following: the execution of certain actions, including conducting intelligence activities, destabilising the situation by committing criminal offences (explosions, murders, assaults, robberies) under the guise of domestic crimes, spreading false information and discrediting the authorities during direct communication with the local population.
The tactical methods of combating sabotage and reconnaissance groups in the territory of the controlled border area are highlighted. The experience of counter-sabotage measures by the security and defence forces of Ukraine, one of the elements of which is an ambush, is studied.
A model of sabotage protection and its use in the educational process of higher education institutions of the Ministry of Internal Affairs of Ukraine is proposed, which provides for a comprehensive combination of command and staff exercises with tactical and special exercises in two stages and involvement of cadets in ensuring public safety and order in border settlements.
Seitsemäsluokkalaisten kolmeen lukuaineeseen sekä ilmiölähtöiseen oppimiseen kohdistamat arvostukset ja odotukset: Motivaatioprofiilit
Authors: Ronkainen Inka , Lonka Kirsti , Vinni-Laakso Janica , Vesterinen Veli-Matti , Salmela-Aro Katariina 
Published: April 2024

Tutkimuksessa tarkastellaan peruskoulun seitsemäsluokkalaisten (N=977) odotuksia ja arvostuksia kolmea lukuainetta sekä ilmiölähtöistä oppimista kohtaan. Aineisto kerättiin syksyllä 2019 sähköisellä motivaatiokysymyksiä sisältäneellä kyselylomakkeella. Henkilösuuntautuneella tutkimusotteella tunnistettiin viisi motivaatioprofiilia odotuksista ja arvostuksista äidinkieltä, matematiikkaa, fysiikkaa ja ilmiölähtöistä oppimista kohtaan. LUMA-orientoituneet (31 %) ilmaisivat korkeinta tehtäväarvoa matematiikkaa ja fysiikkaa kohtaan, Ilmiöorientoituneet (20 %) olivat kiinnostuneempia ilmiölähtöisestä oppimisesta kuin tutkituista oppiaineista, Motivoituneet hyvinvoivat (24 %) olivat kiinnostuneita ilmiölähtöisyydestä sekä mitatuista oppiaineista ja kokivat kaikissa niissä vain vähän kuormitusta. Motivoituneet kuormittuneet (14 %) olivat kiinnostuneita kaikesta, mutta kokivat myös kuormitusta, ja Heikosti motivoituneet (10 %) eivät olleet motivoituneita mistään mitatuista aihepiireistä ja kokivat korkeaa kuormitusta. Tyttöjen ja poikien välillä ei ollut merkitsevää eroa profiileissa. Profiilien välillä tunnistettiin eroja perheen taloustilanteessa ja kaikkien arvosanojen keskiarvoissa. Keskiarvoinen tai heikompi taloustilanne -ryhmään kuului Motivoituneita hyvinvoivia odotettua vähemmän ja Heikosti motivoituneita enemmän. Heikosti motivoituneet -profiilin oppilailla tunnistettiin alhaisin ja Motivoituneilla hyvinvoivilla korkein keskiarvo. Merkittävä osa oppilaista koki ilmiölähtöisen oppimisen kiinnostavana. Tätä tietoa kannattaisi hyödyntää kouluissa: ilmiöön nivominen voisi lisätä oppilaiden motivaatiota myös monen oppiaineen tai laaja-alaisen taidon suhteen.
Analysis of the uterine artery pulsatility index on the day of endometrial transformation and pregnancy outcomes of patients undergoing frozen–thawed embryo transfer
Authors: Li Li , Du Mingze , Wu Sheling , Wen Caiyuzhu , Kong Pingping , Zhang Junwei , Guan Yichun 
Published: April 2024

ObjectiveThe objective was to analyze the impact of the uterine artery pulsatility index (PI) on pregnancy outcomes by measuring uterine artery blood flow on the day of endometrial transformation in patients undergoing frozen–thawed embryo transfer (FET).MethodsThis was a case-control study. In total, 2,036 patients who underwent FET at the Third Affiliated Hospital of Zhengzhou University from October 2019 to September 2020 were included. The patients were divided into a clinical pregnancy group and a nonclinical pregnancy group according to pregnancy outcome. A multivariate logistic regression model was used to analyze the factors affecting the clinical pregnancy rate. The receiver operating characteristic (ROC) curve was used to determine the optimal mean PI cutoff value of 1.75. After 1:1 propensity score matching (PSM), 562 patients were included. For statistical description and analysis, the patients were divided into two groups: a group with a mean PI &gt; 1.75 and a group with a mean PI ≤ 1.75.ResultsThe clinical pregnancy group included 1,218 cycles, and the nonclinical pregnancy group included 818 cycles. There were significant differences in female age (P&lt;0.01), infertility type (P=0.04), baseline follicle-stimulating hormone level (P=0.04), anti-Müllerian hormone (AMH) level (P&lt;0.01), antral follicle count (P&lt;0.01), number of transferred embryos (P=0.045) and type of transferred embryo (P&lt;0.01). There was no significant difference in the mean bilateral PI (1.98 ± 0.34 vs. 1.95 ± 0.35, P=0.10). The multivariate analysis results showed that maternal age (AOR=0.95, 95% CI=0.93-0.98, P&lt;0.01), AMH level (AOR=1.00, 95% CI=1.00-1.01, P=0.045), number of transferred embryos (AOR=1.98, 95% CI=1.47-2.70, P&lt;0.01), and type of transferred embryo (AOR=3.10, 95% CI=2.27-4.23, P&lt;0.01) were independent factors influencing the clinical pregnancy rate. The mean PI (AOR=0.85, 95% CI=0.70-1.05; P=0.13) was not an independent factor influencing the clinical pregnancy rate. Participants were divided into two groups according to the mean PI cutoff value of 1.75, and there was no significant difference between the two groups (P &gt; 0.05).ConclusionIn this study, we found that the uterine artery PI on the day of endometrial transformation in patients undergoing FET is not a good predictor of pregnancy outcomes.
Impact of serum leptin and adiponectin levels on brain infarcts in patients with mild cognitive impairment and Alzheimer’s disease: a longitudinal analysis
Authors: Carbone Giovanni , Bencivenga Leonardo , Santoro Maria Angela , De Lucia Natascia , Palaia Maria Emiliana , Ercolano Erica , Scognamiglio Francesco , Edison Paul , Ferrara Nicola , Vitale Dino Franco , Rengo Giuseppe , Femminella Grazia Daniela 
Published: April 2024

IntroductionThe adipokines leptin and adiponectin have been associated with atherosclerosis and the risk of cerebral infarcts. Pre-clinical studies, however, suggest a protective role against ischemic brain damage. In this study we analyzed the relationship between serum leptin and adiponectin levels and the onset or progression of brain infarcts in subjects with mild cognitive impairment (MCI) and Alzheimer’s disease (AD).MethodsAll data were extracted from the ADNI database. The final population included 566 subjects, with 58 healthy controls, 396 MCI and 112 AD. All patients with available serum leptin and adiponectin levels at baseline were selected. Demographics, neuropsychological test results, CSF biomarkers, regional brain metabolism with FDG-PET data and the number of brain infarcts on longitudinal MRI scans were extracted.ResultsLeptin levels were significantly lower in patients with MCI than controls at baseline, while adiponectin levels were not different between the groups. Multivariate logistic regression analysis at baseline for the presence of brain infarcts showed a predictive value for leptin but not for adiponectin. Multivariate longitudinal analysis showed that age was the only significant predictor of brain infarcts development at 15-year follow-up, while serum leptin and adiponectin levels did not play a role in this population.DiscussionThe evidence on the pathogenetic or protective role of adipokines on ischemic brain damage is mixed. In this MCI and AD population, serum leptin and adiponectin were not associated with the development of brain infarcts; therefore, these results do not support the use of adipokines as biomarkers of cerebrovascular pathology in this population.
Single cell transcriptomic analyses reveal diverse and dynamic changes of distinct populations of lung interstitial macrophages in hypoxia-induced pulmonary hypertension
Authors: Kumar Sushil , Mickael Claudia , Kumar Rahul , Prasad Ram Raj , Campbell Nzali V. , Zhang Hui , Li Min , McKeon B. Alexandre , Allen Thaddeus E. , Graham Brian B. , Yu Yen-Rei A. , Stenmark Kurt R. 
Published: April 2024

IntroductionHypoxia is a common pathological driver contributing to various forms of pulmonary vascular diseases leading to pulmonary hypertension (PH). Pulmonary interstitial macrophages (IMs) play pivotal roles in immune and vascular dysfunction, leading to inflammation, abnormal remodeling, and fibrosis in PH. However, IMs’ response to hypoxia and their role in PH progression remain largely unknown. We utilized a murine model of hypoxia-induced PH to investigate the repertoire and functional profiles of IMs in response to acute and prolonged hypoxia, aiming to elucidate their contributions to PH development.MethodsWe conducted single-cell transcriptomic analyses to characterize the repertoire and functional profiles of murine pulmonary IMs following exposure to hypobaric hypoxia for varying durations (0, 1, 3, 7, and 21 days). Hallmark pathways from the mouse Molecular Signatures Database were utilized to characterize the molecular function of the IM subpopulation in response to hypoxia.ResultsOur analysis revealed an early acute inflammatory phase during acute hypoxia exposure (Days 1-3), which was resolved by Day 7, followed by a pro-remodeling phase during prolonged hypoxia (Days 7-21). These phases were marked by distinct subpopulations of IMs: MHCIIhiCCR2+EAR2+ cells characterized the acute inflammatory phase, while TLF+VCAM1hi cells dominated the pro-remodeling phase. The acute inflammatory phase exhibited enrichment in interferon-gamma, IL-2, and IL-6 pathways, while the pro-remodeling phase showed dysregulated chemokine production, hemoglobin clearance, and tissue repair profiles, along with activation of distinct complement pathways.DiscussionOur findings demonstrate the existence of distinct populations of pulmonary interstitial macrophages corresponding to acute and prolonged hypoxia exposure, pivotal in regulating the inflammatory and remodeling phases of PH pathogenesis. This understanding offers potential avenues for targeted interventions, tailored to specific populations and distinct phases of the disease. Moreover, further identification of triggers for pro-remodeling IMs holds promise in unveiling novel therapeutic strategies for pulmonary hypertension.
Pre-soil fumigation with ammonium bicarbonate and lime modulates the rhizosphere microbiome to mitigate clubroot disease in Chinese cabbage
Authors: Zhang Jinhao , Zhou Xinghai , Zhang Yu , Dai Zhenlin , He Zulei , Qiu Yue , Alharbi Sulaiman Ali , Wei Fangjun , Wei Lanfang , Ahmed Waqar , Ji Guanghai 
Published: April 2024

BackgroundPlasmodiophora brassicae is an ever-increasing threat to cruciferous crop production worldwide.Aims and methodsThis study investigated the impact of pre-soil fumigation with ammonium bicarbonate (N) and lime (NB) to manage clubroot disease in Chinese cabbage through 16S rRNA gene amplification sequencing.ResultsWe found that soil fumigation with N and NB suppressed disease incidence by reducing the soil acidity and population of P. brassicae in the rhizosphere. Minimum disease incidence and maximum relative control effect of about 74.68 and 66.28% were achieved in greenhouse and field experiments, respectively, under the combined application of ammonium bicarbonate and lime (LNB) as compared with N, NB, and control (GZ). Microbial diversity analysis through Miseq sequencing proved that pre-soil fumigation with N, NB, and LNB clearly manipulated rhizosphere microbial community composition and changed the diversity and structure of rhizosphere microbes compared with GZ. Bacterial phyla such as Proteobacteria, Bacteriodetes, and Acidobacteria and fungal phyla including Olpidiomycota and Ascomycota were most dominant in the rhizosphere of Chinese cabbage plants. Soil fumigation with N and NB significantly reduced the abundance of clubroot pathogen at genus (Plasmodiophora) level compared with GZ, while decreased further under combined application LNB. Microbial co-occurrence network analysis showed a highly connected and complex network and less competition for resources among microbes under combined application LNB.ConclusionWe conclude that for environmentally friendly and sustainable agriculture, soil fumigation with combined ammonium bicarbonate and lime plays a crucial role in mitigating Chinese cabbage clubroot disease by alleviating soil pH, reducing pathogen population, and manipulating the rhizosphere microbiome.
Development and performance assessment of novel machine learning models for predicting postoperative pneumonia in aneurysmal subarachnoid hemorrhage patients: external validation in MIMIC-IV
Authors: Li Xinbo , Zhang Chengwei , Wang Jiale , Ye Chengxing , Zhu Jiaqian , Zhuge Qichuan 
Published: April 2024

BackgroundPostoperative pneumonia (POP) is one of the primary complications after aneurysmal subarachnoid hemorrhage (aSAH) and is associated with postoperative mortality, extended hospital stay, and increased medical fee. Early identification of pneumonia and more aggressive treatment can improve patient outcomes. We aimed to develop a model to predict POP in aSAH patients using machine learning (ML) methods.MethodsThis internal cohort study included 706 patients with aSAH undergoing intracranial aneurysm embolization or aneurysm clipping. The cohort was randomly split into a train set (80%) and a testing set (20%). Perioperative information was collected from participants to establish 6 machine learning models for predicting POP after surgical treatment. The area under the receiver operating characteristic curve (AUC), precision-recall curve were used to assess the accuracy, discriminative power, and clinical validity of the predictions. The final model was validated using an external validation set of 97 samples from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database.ResultsIn this study, 15.01% of patients in the training set and 12.06% in the testing set with POP after underwent surgery. Multivariate logistic regression analysis showed that mechanical ventilation time (MVT), Glasgow Coma Scale (GCS), Smoking history, albumin level, neutrophil-to-albumin Ratio (NAR), c-reactive protein (CRP)-to-albumin ratio (CAR) were independent predictors of POP. The logistic regression (LR) model presented significantly better predictive performance (AUC: 0.91) than other models and also performed well in the external validation set (AUC: 0.89).ConclusionA machine learning model for predicting POP in aSAH patients was successfully developed using a machine learning algorithm based on six perioperative variables, which could guide high-risk POP patients to take appropriate preventive measures.
scRNA-seq characterizing the heterogeneity of fibroblasts in breast cancer reveals a novel subtype SFRP4+ CAF that inhibits migration and predicts prognosis
Authors: Ning Lvwen , Quan Chuntao , Wang Yue , Wu Zhijie , Yuan Peixiu , Xie Ni 
Published: April 2024

IntroductionCancer-associated fibroblasts (CAFs) are a diverse group of cells that significantly impact the tumor microenvironment and therapeutic responses in breast cancer (BC). Despite their importance, the comprehensive profile of CAFs in BC remains to be fully elucidated.MethodsTo address this gap, we utilized single-cell RNA sequencing (scRNA-seq) to delineate the CAF landscape within 14 BC normal-tumor paired samples. We further corroborated our findings by analyzing several public datasets, thereby validating the newly identified CAF subtype. Additionally, we conducted coculture experiments with BC cells to assess the functional implications of this CAF subtype.ResultsOur scRNA-seq analysis unveiled eight distinct CAF subtypes across five tumor and six adjacent normal tissue samples. Notably, we discovered a novel subtype, designated as SFRP4+ CAFs, which was predominantly observed in normal tissues. The presence of SFRP4+ CAFs was substantiated by two independent scRNA-seq datasets and a spatial transcriptomics dataset. Functionally, SFRP4+ CAFs were found to impede BC cell migration and the epithelial-mesenchymal transition (EMT) process by secreting SFRP4, thereby modulating the WNT signaling pathway. Furthermore, we established that elevated expression levels of SFRP4+ CAF markers correlate with improved survival outcomes in BC patients, yet paradoxically, they predict a diminished response to neoadjuvant chemotherapy in cases of triple-negative breast cancer.ConclusionThis investigation sheds light on the heterogeneity of CAFs in BC and introduces a novel SFRP4+ CAF subtype that hinders BC cell migration. This discovery holds promise as a potential biomarker for refined prognostic assessment and therapeutic intervention in BC.
Unveiling the potential of SLURP1 protein as a biomarker for prostate cancer screening
Authors: Yang Tianyin , Liu Tianci , Lei Ting , Li Tao , Liu Na , Zhang Man 
Published: April 2024

BackgroundProstate cancer (PCa) develops slowly and lacks obvious symptoms in the early stage, which makes early screening and diagnosis difficult. Urine collection is simple and is an ideal source of biomarkers. In this study, we performed urinary proteomic studies in PCa patients to screen proteins and apply them to the non-invasive early diagnosis of PCa.MethodUrine samples from PCa patients, benign prostatic hyperplasia (BPH) patients and normal control group were collected. Mass spectrometry was used for proteomic analysis and screening target proteins. Western blot and enzyme-linked immunosorbent assay (ELISA) were used to verify the results. Correlations with clinical indicators were explored, and receiver operating characteristic (ROC) curves were drawn to evaluate the value of target proteins in PCa.ResultA total of 1065 proteins were identified. Urinary SLURP1 protein was significantly elevated in patients with PCa compared with normal controls and patients with BPH patients. Western blot and ELISA further verified the expression changes of SLURP1. The immunohistochemical staining results revealed a substantial increase in positive SLURP1 expression within PCa tumor tissue. Correlation analysis showed a positive correlation between the expression level of urine SLURP1 protein and serum PSA. ROC curve analysis of the SLURP1 protein in the urine of both normal individuals and PCa patients is determined to be 0.853 (95% CI=0.754 to 0.954).ConclusionThe concentration of SLURP1 protein in urine of PCa patients is increased, which can serve as a biomarker for screening PCa.