bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modelling rainfall interception losses of three plantations in the Loess Plateau
Authors: Wei Wanyin , Song Xiaoyu , Li Lanjun , Zhao Xinkai , Meng Pengfei , Fu Chong , Wang Long , Li Huaiyou 
Published: March 2024

AbstractThe forest canopy affects the water entering the forest ecosystem by intercepting rainfall. This is especially pertinent in forests that depend on rainfall for their ecological water needs, quantifying and simulating interception losses provide critical insights into their ecological hydrological processes. In the semi‐arid areas of the Loess Plateau, afforestation has become an effective ecological restoration measure. However, the rainfall interception process of these plantations is still unclear. To quantify and model the canopy interception of these plantations, we conducted a two‐year rainfall redistribution measurement experiment in three typical plantations, including a deciduous broadleaf plantation (Robinia pseudoacacia) and two evergreen coniferous plantations (Platycladus orientalis and Pinus tabuliformis). Based on this, the revised Gash model was used to simulate their interception losses, and the model applicability across varying rainfall types was further compared and verified. The experiment clarified the rainfall redistribution in the three plantations, and the proportions of throughfall to gross rainfall in Robinia pseudoacacia, Platycladus orientalis, and Pinus tabuliformis were 84.8%, 70.4%, and 75.6%; corresponding, the stemflow proportions were 2.0%, 2.2%, and 1.8%; the interception losses were 13.2%, 27.4%, and 22.6%, respectively. The dominant rainfall pattern during the experiment was characterized by low‐amounts, moderate‐intensity, and short‐duration, during which the highest interception proportions across the three plantations were observed. We used the Penman‐Monteith equation and the regression method, respectively, to estimate the canopy average evaporation rate of the revised Gash model, finding that the latter provides a closer match to the measured cumulative interception (NSE >0.7). When simulating interception under the three rainfall patterns, the model with the regression method better simulated the cumulative interception and event‐scale interception for Platycladus orientalis and Pinus tabuliformis plantations under the dominant rainfall pattern. The results contribute valuable information to assess the impact of forest rainfall interception on regional hydrologic processes.
Readmission following methotrexate treatment for tubal pregnancy
Authors: Bart Yossi , Regev Noam , Shani Uria , Cohen Bracha , Yossef Fayrooz , Margieh Nadine , Kugelman Nir 
Published: March 2024

AbstractObjectiveTo identify risk factors for readmission following methotrexate treatment for tubal pregnancy.MethodsA retrospective study undertaken in two tertiary medical centers, including all individuals with medically treated tubal pregnancy (N = 511), between December 2009 and June 2021. Individuals with and without readmission following methotrexate treatment were compared. The primary outcome was the readmission rate. Secondary outcomes included the rate of post‐discharge gynecological emergency department visits, tubal rupture rate, and the eventual need for surgical treatment.ResultsReadmission following methotrexate treatment occurred in 224/511 patients (43.8%). Most readmissions were due to abdominal pain or suspicion of treatment failure. Readmitted individuals were more likely to have a history of pelvic inflammatory disease and pretreatment serum human chorionic gonadotropin (hCG) >2000 mIU/mL. Both factors remained significantly associated with higher readmission rates in a logistic regression analysis (adjusted odds ratio [OR] 6.28, 95% confidence interval [CI] 1.30–30.45, and adjusted OR 2.73, 95% CI 1.83–4.07, respectively) after adjustment for age, endometriosis, tubal pathology, abdominal pain, and presence of yolk sac or embryo at diagnosis. A dose‐dependent association was observed between pretreatment serum hCG levels and readmission rate (P < 0.001). Pretreatment hCG levels were also associated with tubal rupture and the eventual need for surgical treatment (P < 0.001 for both). A prediction model using hCG was not sufficiently accurate to predict readmission risk.ConclusionReadmission following methotrexate treatment for tubal pregnancy was independently associated with previous pelvic inflammatory disease and pretreatment serum hCG levels. The latter was also associated with surgical intervention rate.
Evaluating the validity of ChatGPT responses on common obstetric issues: Potential clinical applications and implications
Authors: Peled Tzuria , Sela Hen Y. , Weiss Ari , Grisaru‐Granovsky Sorina , Agrawal Swati , Rottenstreich Misgav 
Published: March 2024

AbstractObjectiveTo evaluate the quality of ChatGPT responses to common issues in obstetrics and assess its ability to provide reliable responses to pregnant individuals. The study aimed to examine the responses based on expert opinions using predetermined criteria, including “accuracy,” “completeness,” and “safety.”MethodsWe curated 15 common and potentially clinically significant questions that pregnant women are asking. Two native English‐speaking women were asked to reframe the questions in their own words, and we employed the ChatGPT language model to generate responses to the questions. To evaluate the accuracy, completeness, and safety of the ChatGPT's generated responses, we developed a questionnaire with a scale of 1 to 5 that obstetrics and gynecology experts from different countries were invited to rate accordingly. The ratings were analyzed to evaluate the average level of agreement and percentage of positive ratings (≥4) for each criterion.ResultsOf the 42 experts invited, 20 responded to the questionnaire. The combined score for all responses yielded a mean rating of 4, with 75% of responses receiving a positive rating (≥4). While examining specific criteria, the ChatGPT responses were better for the accuracy criterion, with a mean rating of 4.2 and 80% of the questions received a positive rating. The responses scored less for the completeness criterion, with a mean rating of 3.8 and 46.7% of questions received a positive rating. For safety, the mean rating was 3.9 and 53.3% of questions received a positive rating. There was no response with an average negative rating below three.ConclusionThis study demonstrates promising results regarding potential use of ChatGPT's in providing accurate responses to obstetric clinical questions posed by pregnant women. However, it is crucial to exercise caution when addressing inquiries concerning the safety of the fetus or the mother.
Students' reading in higher education: Challenges and ways forward
Authors: Bergman Lotta 
Published: March 2024

AbstractThis article deals with reading as a significant challenge for higher education students. The study aimed to understand students' experiences of challenges in reading during their first three semesters at university and how they handled these challenges. It is a qualitative case study built on in‐depth interviews with nine people studying to be primary teachers and their diaries about reading experiences in the academy. The findings show that students experience considerable anxiety and stress regarding finding enough time for reading tasks and comprehending what they read. They soon discover that extensive reading is a prerequisite for writing, questioning, and active participation in lectures and seminars. Despite experiences of lack of support from their teachers, most students identify new approaches and strategies to develop their reading, moving from passive to active engagement with texts. The findings implicate that continuous support from disciplinary experts is crucial for students' reading and writing development. Such support includes inviting students to participate in disciplinary practices, its tools, concepts, ways of thinking, genres used, approaches to reading, and what it means to reason, analyze, and argue in a discipline.
Dynein Light Intermediate Chains Exhibit Different Arginine Methylation Patterns
Authors: Bu Weiwen , Di Jie , Zhao Junkui , Liu Ruming , Wu Yue , Ran Jie , Li Te 
Published: March 2024

ABSTRACTBackgroundThe motor protein dynein is integral to retrograde transport along microtubules and interacts with numerous cargoes through the recruitment of cargo‐specific adaptor proteins. This interaction is mediated by dynein light intermediate chain subunits LIC1 (DYNC1LI1) and LIC2 (DYNC1LI2), which govern the adaptor binding and are present in distinct dynein complexes with overlapping and unique functions.MethodsUsing bioinformatics, we analyzed the C‐terminal domains (CTDs) of LIC1 and LIC2, revealing similar structural features but diverse post‐translational modifications (PTMs). The methylation status of LIC2 and the proteins involved in this modification were examined through immunoprecipitation and immunoblotting analyses. The specific methylation sites on LIC2 were identified through a site‐directed mutagenesis analysis, contributing to a deeper understanding of the regulatory mechanisms of the dynein complex.ResultsWe found that LIC2 is specifically methylated at the arginine 397 residue, a reaction that is catalyzed by protein arginine methyltransferase 1 (PRMT1).ConclusionsThe distinct PTMs of the LIC subunits offer a versatile mechanism for dynein to transport diverse cargoes efficiently. Understanding how these PTMs influence the functions of LIC2, and how they differ from LIC1, is crucial for elucidating the role of dynein‐related transport pathways in a range of diseases. The discovery of the arginine 397 methylation site on LIC2 enhances our insight into the regulatory PTMs of dynein functions.
A Systematic Literature Review on the Use of Dried Biofluid Microsampling in Patients With Kidney Disease
Authors: Lamond Megan K. , Chetwynd Andrew J. , Salama Alan D. , Oni Louise 
Published: March 2024

ABSTRACTBackgroundKidney disease is fairly unique due to the lack of symptoms associated with disease activity, and it is therefore dependent on biological monitoring. Dried biofluids, particularly dried capillary blood spots, are an accessible, easy‐to‐use technology that have seen increased utility in basic science research over the past decade. However, their use is yet to reach the kidney patient population clinically or in large‐scale discovery science initiatives. The aim of this study was to systematically evaluate the existing literature surrounding the use of dried biofluids in kidney research.MethodsA systematic literature review was conducted using three search engines and a predefined search term strategy. Results were summarised according to the collection method, type of biofluid, application to kidney disease, cost, sample stability and patient acceptability.ResultsIn total, 404 studies were identified and 67 were eligible. In total, 34,739 patients were recruited to these studies with a skew towards male participants (> 73%). The majority of samples were blood, which was used either for monitoring anti‐rejection immunosuppressive drug concentrations or for kidney function. Dried biofluids offered significant cost savings to the patient and healthcare service. The majority of patients preferred home microsampling when compared to conventional monitoring.ConclusionThere is an unmet need in bringing dried microsampling technology to advance kidney disease despite its advantages. This technology provides an opportunity to upscale patient recruitment and longitudinal sampling, enhance vein preservation and overcome participation bias in research.
Analytical Performance Evaluation of a Digital Real‐Time PCR for Quantifying Major BCR::ABL1 Transcripts
Authors: Lee Soo Jung , Lee Jong‐Mi , Ahn Ari , Lee Sung‐Eun , Hong Yuna , Lee Gun Dong , Song Hyun‐Woo , Song Min‐Sik , Shin Seung‐Shick , Kim Myungshin , Kim Yonggoo 
Published: March 2024

ABSTRACTBackgroundAccurate quantification of the BCR::ABL1 transcripts is essential for measurable residual disease (MRD) monitoring in chronic myeloid leukemia (CML) after tyrosine kinase inhibitor (TKI) treatment. This study evaluated the newly developed digital real‐time PCR method, Dr. PCR, as an alternative reverse transcription‐PCR (qRT‐PCR) for MRD detection.MethodsThe performance of Dr. PCR was assessed using reference and clinical materials. Precision, linearity, and correlation with qRT‐PCR were evaluated. MRD levels detected by Dr. PCR were compared with qRT‐PCR, and practical advantages were investigated.ResultsDr. PCR detected MRD up to 0.0032%IS (MR4.5) with excellent precision and linearity and showed a strong correlation with qRT‐PCR results. Notably, Dr. PCR identified higher levels of MRD in 12.7% (29/229) of patients than qRT‐PCR, including six cases of MR4, which is a critical level for TKI discontinuation. Dr. PCR also allowed for sufficient ABL1 copies in all cases, while qRT‐PCR necessitated multiple repeat tests in 3.5% (8/229) of cases.ConclusionOur study provides a body of evidence supporting the clinical application of Dr. PCR as a rapid and efficient method for assessing MRD in patients with CML under the current treatment regimen.
Catalytic role of biogenic gold nanoparticles in improving Cr(VI) removal efficiency of biocathode microbial fuel cells
Authors: Tang Shien , Zhuang Xinglei , Zhao Wanqi , Liang Jinhua , Zeng Yang , Xin Fengxue , Dong Weiliang , Jia Honghua , Wu Xiayuan 
Published: March 2024

AbstractBACKGROUNDBiogenic metal nanoparticle‐modified electrodes have a promising prospect for improving the efficiency of microbial fuel cells (MFCs) for hexavalent chromium (Cr(VI))‐containing wastewater treatment. In this study, a graphene (GO) electrode was modified with chemical gold nanoparticles (ChemAu) and biogenic gold nanoparticles (BioAu), respectively, and the two modified electrodes were then used as MFC biocathode electrodes to treat Cr(VI)‐containing wastewater.RESULTSThe results demonstrated that the BioAu/GO biocathode‐based MFC obtained the highest power density (95.78 ± 1.11 mW m−2) and Cr(VI) removal rate (2.17 ± 0.51 mg L−1 h), which were 13.19 and 1.03 times higher than those of the graphite paper biocathode‐based MFC, respectively. The Cr(VI) removal efficiency of the BioAu/GO biocathode‐based MFC under close‐circuit condition reached 87.61 ± 0.19%, which was 3.74 times higher than that recorded under open‐circuit conditions, indicating the critical role of the bioelectrochemical reduction reaction mediated by the BioAu/GO biocathode on Cr(VI) removal.CONCLUSIONThe BioAu/GO electrode first confirmed its superior performance to the ChemAu/GO electrode in Cr(VI)‐reducing MFCs due to its excellent material properties. This study provides a technical reference for the exploration of efficient bioelectrode materials based on biogenic metal nanoparticles for MFCs to treat recalcitrant pollutant‐containing wastewater. © 2024 Society of Chemical Industry (SCI).
The efficacy of bile liquid biopsy in the diagnosis and treatment of biliary tract cancer
Authors: Miura Yoshifumi , Ohyama Hiroshi , Mikata Rintaro , Hirotsu Yosuke , Amemiya Kenji , Mochizuki Hitoshi , Ikeda Junichiro , Ohtsuka Masayuki , Kato Naoya , Omata Masao 
Published: March 2024

AbstractBackgroundDiagnosing biliary tract cancer is difficult because endoscopic retrograde cholangiopancreatography (ERCP) is performed fluoroscopically, and the sensitivity of bile cytology is low. Liquid biopsy of bile using targeted sequencing is expected to improve diagnosis and treatment, but few studies have been conducted. In this study, we examined whether liquid biopsy of bile improves the diagnostic sensitivity of biliary strictures.MethodsA total of 72 patients with biliary strictures who underwent ERCP at Chiba University Hospital between April 2018 and March 2021 were examined. Of these, 43 and 29 were clinically and pathologically diagnosed as having malignant and benign biliary strictures, respectively. We performed targeted sequencing of bile obtained from these patients, and the sensitivity of this method was compared with that of bile cytology. Detection of at least one oncogenic mutation was defined as having malignancy.ResultsThe sensitivity of bile cytology was 27.9%, whereas that of genomic analysis was 46.5%. Comparing bile cytology alone with the combination of cytology and genomic analysis, the latter was more sensitive (53.5%, p < .001). Among the 43 patients with malignant biliary strictures, mutations with FDA‐approved drugs were detected in 11 (26%).ConclusionsLiquid biopsy of bile can potentially diagnose malignancy and detect therapeutic targets.
Ultrasound‐assisted annealing treatment to improve physicochemical and digestive properties of banana flour
Authors: Keeratiburana Thewika , Siangwengwang Nattika , Somphunga Wipha , Fu Tian , Blennow Andreas 
Published: March 2024

AbstractBACKGROUNDBanana flour can provide a solution to people with gluten intolerance, as it is gluten‐free. Native banana flour may have limited functionality in certain applications. In this study, banana flour was modified by ultrasonic (US) and annealing (ANN) treatments at four incubation time spans, namely 12, 24, 36 and 72 h, separately or combined sequentially (US–ANN) to enhance the physicochemical and digestive properties.RESULTSUS led to exposed granular surfaces and damaged non‐starch components. Both treatments, at extended incubation time, increased crystallinity, resulting in a narrower starch gelatinization temperature range. The swelling power was significantly lower for ANN and US–ANN compared to US alone, providing a delay of gelatinization temperature. However, none of the treatments affected the gelatinization enthalpy. Furthermore, US increased peak viscosity, breakdown, final viscosity and setback whereas the opposite results were obtained for ANN and US–ANN. Additionally, US prior to ANN significantly increased the resistant starch (RS) content for annealing times over 24 h, especially for the US–ANN treatment for 72 h, which provided the highest RS content (49.3%) compared to ANN treatment for 72 h (44.0%) and native flour (36.3%).CONCLUSIONSUS prior to ANN treatment offers an alternative method to improve the functional and digestive properties of banana flour, extending the range of applications. © 2024 Society of Chemical Industry.
Increasing Prevalence of Voice Disorders in the USA: Updates in the COVID Era
Authors: Naunheim Matthew R. , DeVore Elliana K. , Huston Molly N. , Song Phillip C. , Franco Ramon A. , Bhattacharyya Neil 
Published: March 2024

ObjectiveTo estimate the current prevalence of voice disorders among adults in the United States; to determine the association of individual factors with voice disorders.MethodsThe 2022 National Health Interview Survey (NHIS) was analyzed to identify adults reporting voice problems in the past 12 months. Demographics were assessed, as well as the duration, severity, and resolution of the voice problem. The relationship between voice problems, gender, lost workdays, and long COVID was investigated. A comparison to the 2012 NHIS was made to determine changes in voice disorder prevalence.Results29.9 million Americans (95%CI[28.3–31.5]) annually report a voice problem, representing 12.2% of the population (95%CI[11.7–12.8%]). Overall, 26.8% and 13.2% reported the severity of their voice problem as moderate or severe, respectively. Only 5.1% (95%CI[4.3–6.0%]) of respondents sought treatment. Most voice problems were resolved within 1 week (53.0%,95%CI[50.9–55.1%]). Females were more likely than males to report a voice problem (14.4% vs. 10.0%,95%CI[13.7–15.1] and [9.3–10.7], respectively). The 17.6 million Americans with long COVID symptoms were more likely to have voice complaints than those without (21.1% vs. 11.6%,95%CI[18.9–23.5%] and [11.1–12.1%], respectively). Lost workdays were not significantly higher for those with voice disorders compared to those without (17.1 vs. 12.9 days,95%CI[12.0–22.1] and [11.0–14.8], respectively).ConclusionsVoice problems affect approximately 1 in 8 adults in the U.S. annually, demonstrating an alarming increased prevalence since 2012 using the same survey methodology. Relatively few individuals seek care for their voice problem, despite significant self‐reported impact. Further study is required regarding the impact of COVID and changes in voice use patterns on voice disorders.Level of Evidence3 Laryngoscope, 134:3713–3718, 2024
Repeatability ofOpen‐MOLLI: An open‐source inversion recovery myocardialT1mapping sequence for fast prototyping
Authors: Gaspar Andreia S. , Silva Nuno A. , Ferreira António M. , Nunes Rita G. 
Published: March 2024

AbstractPurposeTo develop an open‐source prototype of myocardial T1 mapping (Open‐MOLLI) to improve accessibility to cardiac T1 mapping and evaluate its repeatability. With Open‐MOLLI, we aim to enable faster implementation and testing of sequence modifications and to facilitate inter‐scanner and cross‐vendor reproducibility studies.MethodsOpen‐MOLLI is an inversion‐recovery sequence using a balanced SSFP (bSSFP) readout, with inversion and triggering schemes based on the 5(3)3 MOLLI sequence, developed in Pulseq. Open‐MOLLI and MOLLI sequences were acquired in the ISMRM/NIST phantom and 21 healthy volunteers. In 18 of those subjects, Open‐MOLLI and MOLLI were repeated in the same session (test–retest).ResultsPhantom T1 values were comparable between methods, specifically for the vial with reference T1 value most similar to healthy myocardium T1 (T1vial3 = 1027 ms): T1MOLLI = 1011 ± 24 ms versus T1Open‐MOLLI = 1009 ± 20 ms. In vivo T1 estimates were similar between Open‐MOLLI and MOLLI (T1MOLLI = 1004 ± 33 ms vs. T1Open‐MOLLI = 998 ± 52 ms), with a mean difference of −17 ms (p = 0.20), despite noisier Open‐MOLLI weighted images and maps. Repeatability measures were slightly higher for Open‐MOLLI (RCMOLLI = 3.0% vs. RCOpen‐MOLLI = 4.4%).ConclusionThe open‐source sequence Open‐MOLLI can be used for T1 mapping in vivo with similar mean T1 values to the MOLLI method. Open‐MOLLI increases the accessibility to cardiac T1 mapping, providing also a base sequence to which further improvements can easily be added and tested.