bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic Errors in Global Radiosonde Precipitable Water Data from Comparisons with Ground-Based GPS Measurements
Authors: Wang Junhong , Zhang Liangying 
Published: June 2008

Abstract
A global, 10-yr (February 1997–April 2006), 2-hourly dataset of atmospheric precipitable water (PW) was produced from ground-based global positioning system (GPS) measurements of zenith tropospheric delay (ZTD) at approximately 350 International Global Navigation Satellite Systems (GNSS) Service (IGS) ground stations. A total of 130 pairs of radiosonde and GPS stations are found within a 50-km distance and 100-m elevation of each other. At these stations, 14 types of radiosondes are launched and the following 3 types of humidity sensors are used: capacitive polymer, carbon hygristor, and goldbeater’s skin. The PW comparison between radiosonde and GPS data reveals three types of systematic errors in the global radiosonde PW data: measurement biases of the 14 radiosonde types along with their characteristics, long-term temporal inhomogeneity, and diurnal sampling errors of once- and twice-daily radiosonde data. The capacitive polymer generally shows mean dry bias of −1.19 mm (−6.8%). However, the carbon hygristor and goldbeater’s skin hygrometers have mean moist biases of 1.01 mm (3.4%) and 0.76 mm (5.4%), respectively. The protective shield over the humidity sensor boom introduced in late 2000 reduces the PW dry bias from 6.1% and 2.6% in 2000 to 3.9% and −1.14% (wet bias) in 2001 for the Vaisala RS80A and RS80H, respectively. The dry bias in Vaisala radiosondes has larger magnitudes during the day than at night, especially for RS90 and RS92, with a day–night difference of 5%–7%. The time series of monthly mean PW differences between the radiosonde and GPS are able to detect significant changes associated with known radiosonde type changes. Such changes would have a significant impact on the long-term trend estimate. Diurnal sampling errors of twice-daily radiosonde data are generally within 2%, but can be as much as 10%–15% for the once-daily soundings. In conclusion, this study demonstrates that the global GPS PW data are useful for identifying and quantifying several kinds of systematic errors in global radiosonde PW data. Several recommendations are made for future needs of global radiosonde and GPS networks and data.
Using the Radiative Kernel Technique to Calculate Climate Feedbacks in NCAR’s Community Atmospheric Model
Authors: Shell Karen M. , Kiehl Jeffrey T. , Shields Christine A. 
Published: June 2008

Abstract
Climate models differ in their responses to imposed forcings, such as increased greenhouse gas concentrations, due to different climate feedback strengths. Feedbacks in NCAR’s Community Atmospheric Model (CAM) are separated into two components: the change in climate components in response to an imposed forcing and the “radiative kernel,” the effect that climate changes have on the top-of-the-atmosphere (TOA) radiative budget. This technique’s usefulness depends on the linearity of the feedback processes. For the case of CO2 doubling, the sum of the effects of water vapor, temperature, and surface albedo changes on the TOA clear-sky flux is similar to the clear-sky flux changes directly calculated by CAM. When monthly averages are used rather than values from every time step, the global-average TOA shortwave change is underestimated by a quarter, partially as a result of intramonth correlations of surface albedo with the radiative kernel. The TOA longwave flux changes do not depend on the averaging period. The longwave zonal averages are within 10% of the model-calculated values, while the global average differs by only 2%. Cloud radiative forcing (ΔCRF) is often used as a diagnostic of cloud feedback strength. The net effect of the water vapor, temperature, and surface albedo changes on ΔCRF is −1.6 W m−2, based on the kernel technique, while the total ΔCRF from CAM is −1.3 W m−2, indicating these components contribute significantly to ΔCRF and make it more negative. Assuming linearity of the ΔCRF contributions, these results indicate that the net cloud feedback in CAM is positive.
Aggregate Terminal Velocity/Temperature Relations
Authors: Brandes Edward A. , Ikeda Kyoko , Thompson Gregory , Schönhuber Michael 
Published: June 2008

Abstract
Terminal velocities of snow aggregates in storms along the Front Range in eastern Colorado are examined with a ground-based two-dimensional video disdrometer. Power-law relationships for particles having equivalent volume diameters of 0.5–20 mm are computed for the temperatures −1°, −5°, and −10°C. Fall speeds increase with temperature. Comparison with relationships found in the literature suggests that temperature-dependent relations may be surrogates for relations based on aggregate composition (e.g., plates, columns, or dendrites) and the degree of riming.
Academic Underachievement in Children with Epilepsy
Authors: Mitchell Wendy G. , Chavez John M. , Lee Hang , Guzman Bianca L. 
Published: June 2008

Academic achievement was studied in 78 children with epilepsy, ages 5 to 13 years, to determine how seizures, treatment of seizures, and sociocultural factors influence academic achievement. Cognitive abilities were assessed with either the McCarthy Scales of Children's Abilities or the Wechsler Intelligence Scale for Children-Revised. Achievement was measured with the Peabody Individual Achievement Tests. Achievement scores were corrected for cognitive ability (IQ), and underachievement was defined as achievement score 1/2 standard deviation or more below IQ. Information regarding seizure history (severity, duration) and treatment with anticonvulsant drugs was obtained. In addition, the family was extensively interviewed regarding the child's environment, behavior, and demographic background. The Home Observation for Measurement of the Environment (HOME) scale was completed on a home visit. Underachievement was frequent, ranging from 16% (Reading Recognition) to 50% (General Knowledge), but there was no relationship between severity or duration of seizure disorder or total exposure to anticonvulsant medications and achievement. Major determinants of achievement included subscales of the HOME scale, age (older children more likely to be underachieving), and parental education. An equal proportion of newly diagnosed and/or untreated subjects were underachieving compared to those with longstanding epilepsy and anticonvulsant drug treatment. (J Child Neurol 1991;6:65-72).
Building Bridges: Working Together to Understand and Prevent Sexual Abuse
Authors: Knopp Fay Honey 
Published: June 2008

Ours is an evolving discipline; yet from time to time, we need to look back briefly so we can become aware and appreciative of our histories as part of this unique and powerful social movement within the mental health field. We also need to look forward and plan with ATSA how we shall move ahead into the 21st century with new vision, careful strategy, superb resources and training, increased multiculturalism, refined research and its improved dissemination, increased professionalism, and, of equal or greater importance, the continuing vitality, creativity, and the unquenchable energy and spirit of our clinicians and scientists that have brought us this far along the path today. We are a unique social movement unlike any other in the mental health field. We are a social change movement by the very nature of our work. Based on what we now know, we are entering a new and exciting phase of sexual assault prevention possibilities, but only with continued and significant societal attitude and systems change will we garner the required support and resources to continue to move toward building a safer society free of sexual assault. And most importantly, as in the past, we are the people who need to continue to come together to do that work. If we do not do it, it will not be done.
Fc-dependent expression of CD137 on human NK cells: insights into “agonistic” effects of anti-CD137 monoclonal antibodies
Authors: Lin Wei , Voskens Caroline J. , Zhang Xiaoyu , Schindler Daniel G. , Wood Aaron , Burch Erin , Wei Yadong , Chen Lieping , Tian Guoliang , Tamada Koji , Wang Lai-Xi , Schulze Dan H. , Mann Dean , Strome Scott E. 
Published: June 2008

AbstractCD137 (4-1BB) is a costimulatory mol-ecule that can be manipulated for the treatment of cancer and autoimmune disease. Although it is known that agonistic antibodies (mAbs) against CD137 enhance the rejection of murine tumors in a natural killer (NK) cell– and T cell–dependent fashion, the mechanism for NK dependence is poorly understood. In this study, we evaluated the ability of 2 different glycoforms of a chimerized antihuman CD137 mAb, an aglycosylated (GA) and a low fucose form (GG), to react with human NK cells. Both mAbs bound similarly to CD137 and partially blocked the interaction between CD137 and CD137 ligand. However, unlike GA mAb, immobilized GG mAb activated NK cells and enhanced CD137 expression. These effects were seemingly dependent on Fc interaction with putative Fc receptors on the NK-cell surface, as only the immobilized Fc-fragment of GG was required for CD137 expression. Furthermore, CD137 expression could be enhanced with antibodies directed against non-CD137 epitopes, and the expression levels directly correlated with patterns of Fc-glycosylation recognized to improve Fc interaction with Fcγ receptors. Our data suggest that CD137 can be enhanced on NK cells in an Fc-dependent fashion and that expression correlates with phenotypic and functional parameters of activation.
Recruitment of T cells into bone marrow of ITP patients possibly due to elevated expression of VLA-4 and CX3CR1
Authors: Olsson Bob , Ridell Börje , Carlsson Lena , Jacobsson Stefan , Wadenvik Hans 
Published: June 2008

AbstractIn idiopathic thrombocytopenic purpura (ITP), platelets are destroyed in the spleen, liver, and bone marrow (BM) by autoantibodies and cytotoxic T cells. In a DNA microarray screen of peripheral blood T cells, we found that VLA-4, CX3CR1, and CXCR4, involved in T-cell homing, had increased expression in ITP patients compared with controls. However, we only found increased protein expression of VLA-4 on T cells from peripheral blood by flow cytometry. To address a possible recruitment of T cells into the organs involved in platelet destruction, we analyzed T cells in BM. In BM, T-cell surface expression of VLA-4 and CX3CR1 was increased in ITP patients compared with controls. Furthermore, the number of CD3+ T cells in BM, but not in blood, was increased in ITP patients compared with controls. This finding was confirmed by immunohistochemistry of BM biopsies. The number of regulatory T cells (CD4+/CD25bright) was decreased in the BM of ITP patients, whereas Fas expression was increased. In conclusion, ITP is associated with accumulation and activation of T cells in the BM. Recruitment of T cells into the target organ (eg, BM) is plausible and may be facilitated through increased VLA-4 and CX3CR1 expression. These molecules might serve as new treatment targets in ITP.
C. elegans dss-1is functionally conserved and required for oogenesis and larval growth
Authors: Pispa Johanna , Palmén Susanna , Holmberg Carina I , Jäntti Jussi 
Published: June 2008

AbstractBackgroundDss1 (or Rpn15) is a recently identified subunit of the 26S proteasome regulatory particle. In addition to its function in the protein degradation machinery, it has been linked to BRCA2 (breast cancer susceptibility gene 2 product) and homologous DNA recombination, mRNA export, and exocytosis. While the fungal orthologues of Dss1 are not essential for viability, the significance of Dss1 in metazoans has remained unknown due to a lack of knockout animal models.ResultsIn the current study deletion ofdss-1was studied inCaenorhabditis eleganswith adss-1loss-of-function mutant anddss-1directed RNAi. The analysis revealed an essential role fordss-1in oogenesis. In addition,dss-1RNAi caused embryonic lethality and larval arrest, presumably due to loss of thedss-1mRNA maternal contribution. DSS-1::GFP fusion protein localised primarily in the nucleus. No apparent effect on proteasome function was found indss-1RNAi treated worms. However, expression of theC. elegans dss-1in yeast cells deleted for its orthologueSEM1rescued their temperature-sensitive growth phenotype, and partially rescued the accumulation of polyubiquitinated proteins in these cells.ConclusionThe first knockout animal model for the gene encoding the proteasome subunit DSS-1/Rpn15/Sem1 is characterised in this study. In contrast to unicellular eukaryotes, theC. elegans dss-1encodes an essential protein, which is required for embryogenesis, larval growth, and oogenesis, and which is functionally conserved with its yeast and human homologues.
Developmental expression of the alpha-skeletal actin gene
Authors: Bertola Laura D , Ott Elisabeth B , Griepsma Sander , Vonk Freek J , Bagowski Christoph P 
Published: June 2008

Abstract

Background
Actin is a cytoskeletal protein which exerts a broad range of functions in almost all eukaryotic cells. In higher vertebrates, six primary actin isoforms can be distinguished: alpha-skeletal, alpha-cardiac, alpha-smooth muscle, gamma-smooth muscle, beta-cytoplasmic and gamma-cytoplasmic isoactin. Expression of these actin isoforms during vertebrate development is highly regulated in a temporal and tissue-specific manner, but the mechanisms and the specific differences are currently not well understood. All members of the actin multigene family are highly conserved, suggesting that there is a high selective pressure on these proteins.

Results
We present here a model for the evolution of the genomic organization of alpha-skeletal actin and by molecular modeling, illustrate the structural differences of actin proteins of different phyla. We further describe and compare alpha-skeletal actin expression in two developmental stages of five vertebrate species (mouse, chicken, snake, salamander and fish). Our findings confirm that alpha-skeletal actin is expressed in skeletal muscle and in the heart of all five species. In addition, we identify many novel non-muscular expression domains including several in the central nervous system.

Conclusion
Our results show that the high sequence homology of alpha-skeletal actins is reflected by similarities of their 3 dimensional protein structures, as well as by conserved gene expression patterns during vertebrate development. Nonetheless, we find here important differences in 3D structures, in gene architectures and identify novel expression domains for this structural and functional important gene.
Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model
Authors: Chen Yuefeng , Wei Tao , Yan Lei , Lawrence Frank , Qian Hui-Rong , Burkholder Timothy P , Starling James J , Yingling Jonathan M , Shou Jianyong 
Published: June 2008

AbstractBackgroundTumor angiogenesis is a highly regulated process involving intercellular communication as well as the interactions of multiple downstream signal transduction pathways. Disrupting one or even a few angiogenesis pathways is often insufficient to achieve sustained therapeutic benefits due to the complexity of angiogenesis. Targeting multiple angiogenic pathways has been increasingly recognized as a viable strategy. However, translation of the polypharmacology of a given compound to its antiangiogenic efficacy remains a major technical challenge. Developing a global functional association network among angiogenesis-related genes is much needed to facilitate holistic understanding of angiogenesis and to aid the development of more effective anti-angiogenesis therapeutics.ResultsWe constructed a comprehensive gene functional association network or interactome by transcript profiling an in vitro angiogenesis model, in which human umbilical vein endothelial cells (HUVECs) formed capillary structures when co-cultured with normal human dermal fibroblasts (NHDFs). HUVEC competence and NHDF supportiveness of cord formation were found to be highly cell-passage dependent. An enrichment test of Biological Processes (BP) of differentially expressed genes (DEG) revealed that angiogenesis related BP categories significantly changed with cell passages. Built upon 2012 DEGs identified from two microarray studies, the resulting interactome captured 17226 functional gene associations and displayed characteristics of a scale-free network. The interactome includes the involvement of oncogenes and tumor suppressor genes in angiogenesis. We developed a network walking algorithm to extract connectivity information from the interactome and applied it to simulate the level of network perturbation by three multi-targeted anti-angiogenic kinase inhibitors. Simulated network perturbation correlated with observed anti-angiogenesis activity in a cord formation bioassay.ConclusionWe established a comprehensive gene functional association network to model in vitro angiogenesis regulation. The present study provided a proof-of-concept pilot of applying network perturbation analysis to drug phenotypic activity assessment.
Genome-wide survey of allele-specific splicing in humans
Authors: Nembaware Victoria , Lupindo Bukiwe , Schouest Katherine , Spillane Charles , Scheffler Konrad , Seoighe Cathal 
Published: June 2008

Abstract

Background
Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation on the efficiency of mRNA splicing is often difficult to predict, many mutations that cause disease through an effect on splicing are likely to remain undiscovered.

Results
We have combined a genome-wide scan for sequence polymorphisms likely to affect mRNA splicing with analysis of publicly available Expressed Sequence Tag (EST) and exon array data. The genome-wide scan uses published tools and identified 30,977 SNPs located within donor and acceptor splice sites, branch points and exonic splicing enhancer elements. For 1,185 candidate splicing polymorphisms the difference in splicing between alternative alleles was corroborated by publicly available exon array data from 166 lymphoblastoid cell lines. We developed a novel probabilistic method to infer allele-specific splicing from EST data. The method uses SNPs and alternative mRNA isoforms mapped to EST sequences and models both regulated alternative splicing as well as allele-specific splicing. We have also estimated heritability of splicing and report that a greater proportion of genes show evidence of splicing heritability than show heritability of overall gene expression level. Our results provide an extensive resource that can be used to assess the possible effect on splicing of human polymorphisms in putative splice-regulatory sites.

Conclusion
We report a set of genes showing evidence of allele-specific splicing from an integrated analysis of genomic polymorphisms, EST data and exon array data, including several examples for which there is experimental evidence of polymorphisms affecting splicing in the literature. We also present a set of novel allele-specific splicing candidates and discuss the strengths and weaknesses of alternative technologies for inferring the effect of sequence variants on mRNA splicing.
Vav3 oncogene activates estrogen receptor and its overexpression may be involved in human breast cancer
Authors: Lee Kiwon , Liu Yin , Mo Jun Qin , Zhang Jinsong , Dong Zhongyun , Lu Shan 
Published: June 2008

AbstractBackgroundOur previous study revealed that Vav3 oncogene is overexpressed in human prostate cancer, activates androgen receptor, and stimulates growth in prostate cancer cells. The current study is to determine a potential role of Vav3 oncogene in human breast cancer and impact on estrogen receptor a (ERα)-mediated signaling axis.MethodsImmunohistochemistry analysis was performed in 43 breast cancer specimens and western blot analysis was used for human breast cancer cell lines to determine the expression level of Vav3 protein. The impact of Vav3 on breast cancer cell growth was determined by siRNA knockdown of Vav3 expression. The role of Vav3 in ERα activation was examined in luciferase reporter assays. Deletion mutation analysis of Vav3 protein was performed to localize the functional domain involved in ERα activation. Finally, the interaction of Vav3 and ERα was assessed by GST pull-down analysis.ResultsWe found that Vav3 was overexpressed in 81% of human breast cancer specimens, particularly in poorly differentiated lesions. Vav3 activated ERα partially via PI3K-Akt signaling and stimulated growth of breast cancer cells. Vav3 also potentiated EGF activity for cell growth and ERα activation in breast cancer cells. More interestingly, we found that Vav3 complexed with ERα. Consistent with its function for AR, the DH domain of Vav3 was essential for ERα activation.ConclusionVav3 oncogene is overexpressed in human breast cancer. Vav3 complexes with ERα and enhances ERα activity. These findings suggest that Vav3 overexpression may aberrantly enhance ERα-mediated signaling axis and play a role in breast cancer development and/or progression.
Perioperative celecoxib administration for pain management after total knee arthroplasty – A randomized, controlled study
Authors: Huang Yu-Min , Wang Chiu-Meng , Wang Chen-Ti , Lin Wei-Peng , Horng Lih-Ching , Jiang Ching-Chuan 
Published: June 2008

Abstract

Background
Non-steroidal anti-inflammatory drugs (NSAIDs) are recommended for multimodal postoperative pain management. We evaluated opioid-sparing effects and rehabilitative results after perioperative celecoxib administration for total knee arthroplasty.

Methods
This was a prospective, randomized, observer-blind control study. Eighty patients that underwent total knee arthroplasty were randomized into two groups of 40 each. The study group received a single 400 mg dose of celecoxib, one hour before surgery, and 200 mg of celecoxib every 12 hours for five days, along with patient-controlled analgesic (PCA) morphine. The control group received only PCA morphine for postoperative pain management. Visual analog scale (VAS) pain scores, active range of motion (ROM), total opioid use and postoperative nausea/vomiting were analyzed.

Results
Groups were comparable for age, pre-operative ROM, operation duration and intraoperative blood loss. Resting VAS pain scores improved significantly in the celecoxib group, compared with controls, at 48 hrs (2.13 ± 1.68 vs. 3.43 ± 1.50, p = 0.03) and 72 hrs (1.78 ± 1.66 vs. 3.17 ± 2.01, p = 0.02) after surgery. Active ROM also increased significantly in the patients that received celecoxib, especially in the first 72 hrs [40.8° ± 17.3° vs. 25.8° ± 11.5°, p = 0.01 (day 1); 60.7° ± 18.1° vs. 45.0° ± 17.3°, p = 0.004 (day 2); 77.7° ± 15.1° vs. 64.3° ± 16.9°, p = 0.004 (day 3)]. Opioid requirements decreased about 40% (p = 0.03) in the celecoxib group. Although patients suffering from post-operative nausea/vomiting decreased from 43% in control group to 28% in celecoxib group, this was not significant (p = 0.57). There were no differences in blood loss (intra- and postoperative) between the groups. Celecoxib resulted in no significant increase in the need for blood transfusions.

Conclusion
Perioperative celecoxib significantly improved postoperative resting pain scores at 48 and 72 hrs, opioid consumption, and active ROM in the first three days after total knee arthroplasty, without increasing the risks of bleeding.

Trial registration
Clinicaltrials.gov NCT00598234