bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hereditary Gingival Fibromatosis: Report of a Five‐Generation Family Using Cellular Proliferation Analysis
Authors: Martelli‐Junior H. , Lemos D.P. , Silva C.O. , Graner E. , Coletta R.D. 
Published: December 2005

Background: Hereditary gingival fibromatosis (HGF) is an uncommon condition characterized by an accumulation of extracellular matrix resulting in a fibrotic enlargement of the gingiva. The goal of this article is to describe one kindred affected with HGF and discuss the diagnosis, treatment, and control of the disease. The pattern of inheritance, histopathologic characteristics, and proliferative potential of epithelial and mesenchymal cells of HGF are also emphasized.Methods: To characterize the pattern of inheritance and the clinical appearance of gingival overgrowth, 117 family members were examined. The recurrence risk was estimated by the use of a genetic analysis program. Immunohistochemistry against the proliferating cell nuclear antigen (PCNA) and pKi‐67 was performed to assess cellular proliferation of normal gingiva (NG) and HGF cells.Results: Examination of the family pedigree demonstrated an autosomal dominant trait of inheritance, and a sibling recurrence risk of 0.085 and an offspring recurrence risk of 0.078, indicating that HGF was a consequence of genetic alteration with low penetrance. Unaffected and affected members transmitted the disease to their offspring. The affected patients showed a generalized but mild gingival overgrowth. Surgical treatment consisted of a combination of gingivectomy and gingivoplasty. Histologic examination showed that the gingival lesions of all patients were quite similar, with increased amounts of collagen fiber bundles in the connective tissue. Immunohistochemistry revealed that the proliferative potential of epithelial cells was significantly higher in the HGF group compared to the NG group, whereas mesenchymal cells from both groups were negative for the proliferative markers.Conclusion: Our data demonstrated that, in the studied family, HGF is transmitted by an autosomal dominant pattern with incomplete disease penetrance, and although the gingival enlargement resulted from an excessive accumulation of collagen fibers, HGF is characterized by an increase in the proliferation rate of epithelial cells.
Papillary Squamous Cell Carcinoma Presenting on the Gingiva
Authors: Khan Saba M. , Gossweiler Michael K. , Zunt Susan L. , Edwards Michael D. , Blanchard Steven B. 
Published: December 2005

Background: Papillary squamous cell carcinoma (PSCC) is a rare variant of squamous cell carcinoma. PSCC can occur as either an in situ or invasive tumor. The sites of occurrence in order of the most to least prevalent are the larynx, oropharynx, and nasopharynx. We present an unusual case of PSCC occurring on the gingiva.Methods: A 72‐year‐old white female presented with a chief complaint of tooth mobility in the right posterior mandible. Clinical examination revealed a Miller's Class 2 mobility of tooth #28 along with an erythematous, papillary appearance of the lingual gingiva on teeth #27, #28, and #29. Her dental history revealed treatment of tooth #28 with locally delivered antibiotics. Her medical history revealed a diagnosis of breast cancer 8 years prior to examination that was treated with partial mastectomy and radiation therapy. An initial differential clinical diagnosis of verrucous carcinoma or metastatic carcinoma was made.Results: Surgical therapy included extraction of tooth #28 and an excisional biopsy of the lesion on the lingual gingiva. Microscopic evaluation of the gingival specimen revealed a neoplastic papillary proliferation of the surface epithelium with a thick layer of parakeratin, deep parakeratin‐lined crypts, and a thickened spinous cell layer along with islands and strands of malignant epithelium. The microscopic appearance of the lesion was characteristic for PSSC. The patient's tumor was removed via a block resection that included teeth #27 through #31 and a radical neck dissection.Conclusions: This is a case report of PSCC occurring on the gingiva. This report demonstrates that, even though oral cancers involving the periodontium are a relatively rare occurrence, periodontists cannot be complacent about the diagnosis of periodontal bone loss. It also highlights the importance of utilizing a histopathologic examination to confirm the clinical diagnosis for any suspicious lesion.
Effects of pasteurization of colostrum on subsequent serum lactoferrin concentration and neutrophil superoxide production in calves
Authors: Lakritz Jeffrey , Tyler Jeff W. , Hostetler Douglas E. , Marsh Antoinette E. , Weaver Dusty M. , Holle Julie M. , Steevens Barry J. , Denbigh John L. 
Published: December 2005

AbstractObjective—To determine the effects of pasteurization of colostrum on serum lactoferrin concentration and neutrophil oxidative function by comparing values from calves given pasteurized (76 C, 15 minutes) colostrum versus calves given fresh frozen colostrum.Animals—8 Holstein bull calves were used to study the effects of pasteurization of colostrum on the absorption of lactoferrin and neutrophil oxidative burst. Three additional calves were used to study the effect of exogenous lactoferrin on neutrophil oxidative burst.Methods—Calves were fed fresh frozen or heat pasteurized colostrum (76 C for 15 minutes) via esophageal feeder within 4 hours of birth. Neutrophils were isolated from whole blood samples. Neutrophil oxidative burst was induced by phorbol ester (300 ng/ml) stimulation of cells (1 × 106cells) at 37 C. Serum lactoferrin concentrations were compared, using immunoblot analysis. Serum IgG concentrations were determined by radial immunoassay. Comparisons were made between the use of the 2 types of colostrum in calves by measuring subsequent serum IgG and lactoferrin concentrations and neutrophil superoxide production.Results—Serum IgG and lactoferrin concentrations increased more in calves receiving fresh frozen colostrum. Neutrophil superoxide production was higher in neutrophils prepared from calves receiving fresh frozen colostrum. Colostral lactoferrin addition to neutrophil incubations resulted in increased oxidative burst.Conclusions and Clinical Relevance—Compared with calves given fresh frozen colostrum, calves given pasteurized colostrum had decreased serum IgG and lactoferrin concentrations and neutrophil superoxide production 24 hours after administration. These results suggest that pasteurizing bovine colostrum at 76 C for 15 minutes has substantial effects on passive transfer of proteins and neutrophil function. (Am J Vet Res2000;61:1019–1025)
Use of an extracorporeal circuit to evaluate effects of ischemia and reperfusion of the equine large colon
Authors: Hoogmoed Linda M. Van , Snyder Jack R. , Nieto Jorge E. , Vatistas Nicholas J. , Harmon Faye A. 
Published: December 2005

AbstractObjective—To determine efficacy of an extracorporeal circuit to maintain a segment of equine large colon for 3.5 hours and to evaluate the effect of low arterial flow on histologic and metabolic variables.Sample Population—Segments of large colon from 15 healthy adult horses.Procedure—The pelvic flexure was surgically removed and maintained in an isolated circuit. In the control group, tissue was evaluated for 3.5 hours, whereas in the low-flow group, arterial flow was reduced to 20% of baseline for 40 minutes followed by 2 hours of reperfusion. Various metabolic and hemodynamic variables were evaluated at 30-minute intervals. Effects of nitric oxide (NO) and L-N-nitro-arginine- methyl-ester (L-NAME) on contractile activity were determined, and histomorphologic evaluation was performed at the completion of the study.Results—Low-flow ischemia with reperfusion caused significant histomorphologic differences, compared with the control group. In the low-flow group, significant differences included reduction in PaCO2, reduction in bicarbonate concentrations, increase in PaO2, and an increase in base deficit in arterial and venous blood samples. Other significant differences included increases in PCV, protein concentration, total WBC count, and albumin clearance for the low-flow group. Differences were not detected in inhibitory activity of the low-flow group relative to the control tissue with or without addition of NO and L-NAME.Conclusion—The extracorporeal circuit maintained a segment of equine intestine for 3.5 hours and can be used to simulate ischemic injury. The extracorporeal circuit provides the potential to investigate pharmaceutic agents that can minimize intestinal injury. (Am J Vet Res2000;61:1042–1051)
Effects of supplemental parenteral administration of vitamin E and selenium to Jerseys and Holsteins during the nonlactating period
Authors: Bass II Roger T. , Swecker William S. , Stallings Charles C. 
Published: December 2005

AbstractObjective—To determine effects of breed and supplemental administration of vitamin E and selenium (Se) during late gestation on circulating concentrations of these micronutrients in periparturient Jerseys and Holsteins.Design—Randomized controlled clinical study.Animals—16 Jersey and 36 Holstein cows.Procedure—Cows were allotted to blocks on the basis of breed and expected parturition date. Cows within blocks were randomly assigned to be given vitamin E or Se parenterally 3 to 4 weeks prior to anticipated parturition in a 2 × 2 factorial design.Results—Results of ANOVA indicated Jerseys had higher blood concentrations of Se and lower serum concentrations of vitamin E than Holsteins at the end of lactation. Jerseys had higher blood concentrations of Se than Holsteins 3 to 4 weeks prior to parturition and at parturition. Selenium administration increased blood concentrations of Se at parturition. Administration of nutrients did not affect serum concentrations of vitamin E at parturition or 2 to 3 weeks after parturition or blood concentrations of Se 2 to 3 weeks after parturition.Conclusions and Clinical Relevance—Jerseys and Holsteins consuming rations of comparable Se content differ in blood concentrations of Se during the nonlactating period, suggesting breed-related differences in Se metabolism during late lactation and the nonlactating period. Parenteral administration of Se 3 to 4 weeks prior to anticipated parturition increased blood concentrations of Se at parturition; however, Se concentrations of both groups at parturition were considered within the reference range for clinically normal cattle. (Am J Vet Res2000;61:1052–1056)
Comparison of the fit and geometry of reconstruction of femoral components of four cemented canine total hip replacement implants
Authors: Schulz Kurt S. , Nielsen Cheri , Stover Susan M. , Kass Philip H. 
Published: December 2005

AbstractObjective—To compare fit and geometry of reconstruction of femoral components of 4 canine cemented total hip replacement implants and determine which implants are most compatible with current principles of cemented arthroplasty.Sample Population—Paired femurs from 16 adult mixed-breed dogs.Procedure—Femurs were prepared for femoral stem implantation of either the Bardet, BioMedtrix, Mathys, or Richards II implant. Mediolateral and craniocaudal radiographs were obtained with femoral components in situ. Cross-sectional analysis of implant fit was performed on transected cemented specimens. Computer-aided analyses of digitized images were performed.Results—The Bardet and Richards II implants reconstructed the original femoral head position significantly better than the other 2 implants. None of the implants allowed neutralization of the implant axis in the sagittal plane or were routinely centralized in the femoral canal. The Bardet implant had the smallest minimum distal tip offset in the sagittal plane. Greatest tip to cortex distance was provided by the Richards II implant in the transverse plane and the Mathys implant in the sagittal plane. The thinnest cement mantle regions for all implants were in the central longitudinal third of the femoral stem.Conclusions and Clinical Relevance—The Bardet and BioMedtrix implants had stem design characteristics that were most compatible with principles of cemented stem fixation. None of the implants completely satisfied the theoretically optimal conditions of centralization and neutralization of the femoral stem. Innovative design modifications, therefore, may be needed if these conditions are important to the longterm success of canine total hip replacement. (Am J Vet Res2000;61:1113–1121)
Effect of pyloric blockade and infusion of histamine or pentagastrin on gastric secretion in horses
Authors: Kitchen Diane L. , Burrow J. A. , Heartless Christie S. , Merritt A. M. 
Published: December 2005

AbstractObjective—To determine the origin of the nonacid (nonparietal) component of gastric secretions in horses induced by pentagastrin infusion.Animals—6 horses.Procedure—A Latin square design was used, involving 6 horses, 3 treatments, and 2 duodenal intubation conditions (catheter with balloon to obstruct pylorus [B] or without balloon allowing movement of contents between stomach and duodenum [NB]). Each horse had an indwelling gastric cannula and a catheter positioned in the duodenum. Gastric and duodenal contents were collected during 15-minute periods. Each experiment consisted of serial collection periods: baseline; infusion of pyrilamine maleate (1 mg/kg of body weight, IV); not treated; and IV infusion of saline (0.9% NaCl) solution alone, saline solution containing pentagastrin (6 µg/kg·h), or saline solution containing histamine (30 µg/kg·h). Volume of samples was recorded, and electrolyte concentrations were measured.Results—Pentagastrin and histamine stimulated maximal acid output; however, during NB conditions, pentagastrin-induced concentration of hydrogen ions was significantly less than during histamine or pentagastrin infusions during B conditions. The large volume produced in response to pentagastrin during NB conditions was accompanied by increased sodium ion output that was greater than for pentagastrin during B conditions, but both values were significantly greater than values for histamine during B or NB conditions.Conclusions and Clinical Relevance—Nonparietal secretions collected during IV infusion of pentagastrin are duodenal in origin. Reflux of duodenal contents into the stomach of horses is enhanced by pentagastrin. Flow of duodenal contents into the stomach could have implications in the pathogenesis of ulcers in horses. (Am J Vet Res2000;61:1133–1139)
Detection of cell detachment activity induced by Moraxella bovis
Authors: Marrion Ruth M. , Riley Lela K. 
Published: December 2005

AbstractObjective—To characterize the effect that filtrate obtained from cultures ofMoraxella bovishas on cultured corneal epithelial cells and other types of cultured mammalian cells.Sample Population—Cultured hamster corneal epithelial cells, bovine epithelial cells, and several transformed cell lines exposed to culture filtrate from a pathogenic isolate ofM bovis.Procedure—Moraxella boviswas cultured, and bacteria were removed by filtration. The resulting bacterial culture filtrate was incubated with various types of cultured cells, and effects of the filtrate on detachment of various mammalian cell types was quantified by the use of neutral red dye. Additionally, bacterial culture filtrate was treated with protease inhibitors as well as trypsin and heat prior to incubation with cultured mammalian cells.Results—Bacterial culture filtrate ofM boviscaused all types of cultured cells to detach from each other and from the substrate, with the maximal effect evident 2 hours after initiating incubation. Detached cells were alive, and detachment was reversible. Serine protease inhibitors (phenylmethylsulfonylfluoride and α2-macroglobulin) inhibited cell detachment attributable to bacterial culture filtrate. Heating and treatment with trypsin also inhibited cell detachment.Conclusions and Clinical Relevance—Moraxella bovisproduces a soluble factor that causes reversible detachment of cultured cells. This activity may play a role in the pathogenesis of infectious bovine keratoconjunctivitis. (Am J Vet Res2000;61:1145–1149)
Lesions and effects of location for administration of clostridial bacterin-toxoid vaccines on growth performance and eating and drinking behaviors in newly arrived calves at a feedlot
Authors: Buhman Marilyn J. , Perino Louis J. , Galyean Michael L. , Montgomery Ted H. , Swingle R. Spencer 
Published: December 2005

AbstractObjectives—To determine the effect of location for administration of clostridial vaccines on behavior, growth performance, and health of calves at a feedlot, the relative risk of calves developing an injection-site reaction or being misdiagnosed as having bovine respiratory disease complex (BRDC), and the percentage of subcutaneous injection-site reactions that were detectable on carcasses after the hides were removed.Animal—170 newly arrived calves at a feedlot.Procedure—Eating and drinking behaviors of calves during the initial 57 days after arrival were observed at a commercial feedlot, using an electronic monitoring system. Calves were assigned randomly to receive a clostridial vaccine (base of ear or neck). Data on reactions at the injection site were collected.Results—Mean daily gain (MDG) for the initial 57 days did not differ significantly between treatments. Risk of being misdiagnosed as having BRDC was not associated with location for administration of vaccine. Calves vaccinated in the base of the ear were at higher risk of having an injection-site reaction at day 57 or at slaughter. Eighty-nine percent (95% confidence interval, 52 to 100%) of injection-site reactions in the neck could not be located on the carcasses after hides were removed. Calves vaccinated in the neck drank significantly fewer times per day during the first 57 days than calves vaccinated in the base of the ear.Conclusions and Clinical Relevance—Location for administration of a clostridial vaccine did not significantly affect health, growth performance, or eating behavior. Most subcutaneous injection-site reactions were not detectable after the hide was removed. (Am J Vet Res2000;61:1169–1172)
Effects of general anesthesia and surgery on renal function in healthy dogs
Authors: Lobetti Remo , Lambrechts Nicolaas 
Published: December 2005

AbstractObjectives—To evaluate renal function in healthy dogs undergoing general anesthesia and ovariohysterectomy without concurrent IV administration of fluids.Animals—35 healthy client-owned dogs.Procedure—Dogs were medicated with promazine hydrochloride (0.05 mg/kg of body weight, SC) approximately 45 minutes before induction of anesthesia with thiopental sodium (10 to 15 mg/kg, IV). Anesthesia was maintained with 2% halothane in oxygen. Ovariohysterectomies were performed by senior veterinary students under the direct supervision of a veterinary surgeon. Renal function was assessed (serum urea and creatinine concentrations, fractional clearance of sodium, urine alkaline phosphatase [ALP] and γ-glutamyltransferase [GGT] activities, urine specific gravity, and enumeration of renal tubular epithelial cells in urine sediment) prior to and 24 and 48 hours after surgery.Results—Duration of general anesthesia ranged from 80 to 310 minutes. Urine specific gravity and ALP activity and serum urea and creatinine concentrations did not change over time. Fractional clearance of sodium decreased 24 and 48 hours after surgery, whereas urine GGT activity and the ratio of urine GGT activity to urine creatinine concentration increased 24 hours after surgery, compared with presurgery values. Renal tubular epithelial cells increased in number in urine sediment from 11 of 35 (31.4%) dogs and 5 of 35 (14.3%) dogs 24 and 48 hours after surgery, respectively. However, this increase was not clinically relevant.Conclusions and Clinical Relevance—Intravenous administration of fluids to healthy dogs undergoing general anesthesia and elective surgery may not be necessary for maintenance of renal homeostasis. (Am J Vet Res2000;61:121–124)
Dual-energy X-ray absorptiometry measurement of bone-mineral density in the distal aspect of the limbs in racing Greyhounds
Authors: Emmerson Terrance D. , Lawes Timothy J. , Goodship Allen E. , Rueux-Mason Caroline , Muir Peter 
Published: December 2005

AbstractObjective—To determine those bones in the distal aspect of the limbs of Greyhounds with fatigue fractures that have the greatest left-to-right differences in bone-mineral density (BMD).Sample Population—Limbs obtained from 20 Greyhounds.Procedure—Dual-energy x-ray absorptiometry (DXA) of the distal aspect of each limb and isolated bones from 10 dogs with a fracture of the central tarsal bone (CTB) of the right pelvic limb was performed. Highresolution scanning was performed on excised bones, and BMD measurements of CTB also were obtained from limbs of dogs without a CTB fracture.Results—The BMD of the accessory carpal bone and calcaneus was not significantly different from the BMD of those bones in the contralateral limb. Although BMD of the CTB of the entire right pelvic limb and isolated bones from dogs with a CTB fracture was lower, compared with values for the entire left pelvic limb, values for isolated CTB from dogs without a CTB fracture were not significantly different. Metacarpal or metatarsal and thoracic or pelvic limb significantly affected BMD for measurements of the entire limb and isolated bones. Left-to-right differences in BMD were greatest for metacarpal 5.Conclusions and Clinical Relevance—Asymmetric adaptive remodeling of metacarpal 5 can be detected by DXA. The potentially confounding effects of CTB fracture and unknown racing history made it difficult to interpret BMD changes in the CTB of these specimens. Densitometry could be developed as an in vivo assessment for risk of fractures in racing Greyhounds. (Am J Vet Res2000;61:1214–1219)
Effects of xylazine hydrochloride during isoflurane-induced anesthesia in horses
Authors: Steffey Eugene P. , Pascoe Peter J. , Woliner Michael J. , Berryman Edwin R. 
Published: December 2005

AbstractObjective—To quantitate dose- and time-related anesthetic-sparing effects of xylazine hydrochloride (XYL) during isoflurane-induced anesthesia in horses and to characterize selected physiologic responses of anesthetized horses to administration of XYL.Animals—6 healthy adult horses.Procedure—Horses were anesthetized 2 times to determine the minimum alveolar concentration (MAC) of isoflurane in O2and to characterize the anestheticsparing effect (MAC reduction) after IV administration of XYL (0.5 and 1 mg/kg of body weight, random order). Selected measures of cardiopulmonary function, blood glucose concentrations, and urinary output also were measured during the anesthetic studies.Results—Isoflurane MAC (mean ± SEM) was reduced by 24.8 ± 0.5 and 34.2 ± 1.9% at 42 ± 7 and 67 ± 10 minutes, respectively, after administration of XYL at 0.5 and 1 mg/kg. Amount of MAC reduction by XYL was dose- and time-dependent. Overall, cardiovascular and respiratory values varied little among treatments. Administration of XYL increased blood glucose concentration; the magnitude of change was dose- and time-dependent. Urine volume increased but not significantly.Conclusions and Clinical Relevance—Administration of XYL reduced the anesthetic requirement for isoflurane in horses. The magnitude of the decrease is dose- and time-dependent. Administration of XYL increases blood glucose concentration in anesthetized horses in a dose-related manner. (Am J Vet Res2000;61:1225–1231)