bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global acetylome profiling indicates EPA impedes but OA promotes prostate cancer motility through altered acetylation of PFN1 and FLNA
Authors: He Chao , Chen Xiuyuan , Chen Ying , Sun Jianying , Qi Manting , Rocha Sonia , Wang Mu 
Published: March 2024

AbstractProstate cancer (PCa) is one of the leading causes of cancer morbidity and mortality in men. Metastasis is the main cause of PCa‐associated death. Recent evidence indicated a significant reduction in PCa mortality associated with higher ω‐3 polyunsaturated fatty acids (PUFAs) consumption. However, the underlying mechanisms remained elusive. In this study, we applied global acetylome profiling to study the effect of fatty acids treatment. Results indicated that oleic acid (OA, monounsaturated fatty acid, MUFA, 100 µM) elevates while EPA (eicosapentaenoic acid, 100 µM) reduces the acetyl‐CoA level, which alters the global acetylome. After treatment, two crucial cell motility regulators, PFN1 and FLNA, were found with altered acetylation levels. OA increased the acetylation of PFN1 and FLNA, whereas EPA decreased PFN1 acetylation level. Furthermore, OA promotes while EPA inhibits PCa migration and invasion. Immunofluorescence assay indicated that EPA impedes the formation of lamellipodia or filopodia through reduced localization of PFN1 and FLNA to the leading edge of cells. Therefore, perturbed acetylome may be one critical step in fatty acid‐affected cancer cell motility. This study provides some new insights into the response of ω‐3 PUFAs treatment and a better understanding of cancer cell migration and invasion modulation.
Oncology clinicians' feelings towards patients presented in supervision: A pre‐post assessment using the feeling word checklist
Authors: Stiefel Friedrich , Bourquin Céline , Wild Beate , Schellberg Dieter , Michaud Laurent 
Published: March 2024

AbstractObjectiveClinical supervision of oncology clinicians by psycho‐oncologists is an important means of psychosocial competence transfer and support. Research on this essential liaison activity remains scarce. The aim of this study was to assess the impact of supervision on oncology clinicians' feelings towards patients presented in supervision.MethodsOncology clinicians' (n = 23) feelings towards patients presented in supervision were assessed with the Feeling Word Checklist (FWC). The FWC was filled in by supervisees prior and after their supervision sessions (n = 91), which were conducted by experienced supervisors (n = 6). Pre‐ post‐modification of feelings was evaluated based on a selection of FWC items, which were beforehand considered as likely to change in a beneficial supervision. Items were evaluated on session level using t‐tests for dependent groups. Composite scores were calculated for feelings expected to raise and feelings expected to decrease and analysed on the level of supervisees.ResultsFeelings related to threats, loss of orientation or hostility such as “anxious”, “overwhelmed”, “impotent”, “confused”, “angry”, “depreciated” and “guilty” decreased significantly after supervision, while feelings related to the resume of the relationship (“attentive”, “happy”), a better understanding of the patient (“empathic”), a regain of control (“confident”) and being “useful” significantly increased. Feeling “interested” and “calm” remained unchanged. Significant increase or decrease in the composite scores for supervisees confirmed these results.ConclusionsThis study demonstrates modification of feelings towards patients presented in supervision. This modification corresponds to the normative, formative, and especially restorative function (support of the clinician) of supervision.
Translation, validity and reliability of the pain attitudes and beliefs scale for physiotherapists in French
Authors: Soreau Alexis , Ferey Cassandre , Hardouin Jean‐Benoit , Draper‐Rodi Jerry , Sarzeaud Robert , Benoist Hélène , Ostelo Raymond W. , Merdy Olivier 
Published: March 2024

AbstractBackgroundThe Pain Attitudes and Beliefs Scale for Physiotherapists (PABS‐PT) questionnaire evaluates manual therapists' biomedical and biopsychosocial beliefs regarding the management of chronic low back pain. Its usage in clinical settings is an important step in the implementation of national guidelines and policies to improve patient management.ObjectivesThe objective of this study was to translate the PABS‐PT questionnaire into French, to adapt it culturally, and to conduct a psychometric analysis.DesignQualitative and cross‐sectional study.MethodThe translation process followed published guidelines with cross‐cultural validation by an expert committee. We followed a forward and backward translation procedure and an expert committee, including the original author of the questionnaire and a linguistics expert ensuring good cultural adaptation, issued a finalised version. Psychometric analysis of the French version of the questionnaire was conducted among 390 French manual therapists in two phases. The first phase evaluated structural validity as well as external validity compared with the TSK and BBQ questionnaires. Then, reliability and scalability were analysed. The second phase evaluated test‐retest reproducibility by sending the same questionnaire 3 months later.ResultsThe validity study revealed three subscales: the classic biomedical subscale and two subscales for biopsychosocial beliefs (aetiology of pain and physical activity). With 21 items in total for the PABS‐PT‐FR, the structural validity scores were good (BM: alpha = 0.82, H = 0.38; Physical Activity: alpha = 0.62, H = 0.32; Aetiology of Pain: alpha = 0.55, H = 0.29).ConclusionsThis study provides a validated tool to assess French physiotherapists' and, more generally, healthcare providers' beliefs about chronic low back pain, with a new insight into the BPS subscale internal construct.
Identification and neuroprotective properties of NA‐184, a calpain‐2 inhibitor
Authors: Baudry Michel , Wang Yubin , Bi Xiaoning , Luo Yun Lyna , Wang Zhijun , Kamal Zeechan , Shirokov Alexander , Sullivan Ed , Lagasca Dennis , Khalil Hany , Lee Gary , Fosnaugh Kathy , Bey Philippe , Mehdi Shujaath , Coulter Greg 
Published: March 2024

AbstractOur laboratory has shown that calpain‐2 activation in the brain following acute injury is directly related to neuronal damage and the long‐term functional consequences of the injury, while calpain‐1 activation is generally neuroprotective and calpain‐1 deletion exacerbates neuronal injury. We have also shown that a relatively selective calpain‐2 inhibitor, referred to as C2I, enhanced long‐term potentiation and learning and memory, and provided neuroprotection in the controlled cortical impact (CCI) model of traumatic brain injury (TBI) in mice. Using molecular dynamic simulation and Site Identification by Ligand Competitive Saturation (SILCS) software, we generated about 130 analogs of C2I and tested them in a number of in vitro and in vivo assays. These led to the identification of two interesting compounds, NA‐112 and NA‐184. Further analyses indicated that NA‐184, (S)‐2‐(3‐benzylureido)‐N‐((R,S)‐1‐((3‐chloro‐2‐methoxybenzyl)amino)‐1,2‐dioxopentan‐3‐yl)‐4‐methylpentanamide, selectively and dose‐dependent inhibited calpain‐2 activity without evident inhibition of calpain‐1 at the tested concentrations in mouse brain tissues and human cell lines. Like NA‐112, NA‐184 inhibited TBI‐induced calpain‐2 activation and cell death in mice and rats, both male and females. Pharmacokinetic and pharmacodynamic analyses indicated that NA‐184 exhibited properties, including stability in plasma and liver and blood–brain barrier permeability, that make it a good clinical candidate for the treatment of TBI.
Development of a physiologically‐based pharmacokinetic model to simulate the pharmacokinetics of intramuscular antiretroviral drugs
Authors: Bettonte Sara , Berton Mattia , Battegay Manuel , Stader Felix , Marzolini Catia 
Published: March 2024

AbstractThere is growing interest in the use of long‐acting (LA) injectable drugs to improve treatment adherence. However, their long elimination half‐life complicates the conduct of clinical trials. Physiologically‐based pharmacokinetic (PBPK) modeling is a mathematical tool that allows to simulate unknown clinical scenarios for LA formulations. Thus, this work aimed to develop and verify a mechanistic intramuscular PBPK model. The framework describing the release of a LA drug from the depot was developed by including both the physiology of the injection site and the physicochemical properties of the drug. The framework was coded in Matlab® 2020a and implemented in our existing PBPK model for the verification step using clinical data for LA cabotegravir, rilpivirine, and paliperidone. The model was considered verified when the simulations were within twofold of observed data. Furthermore, a local sensitivity analysis was conducted to assess the impact of various factors relevant for the drug release from the depot on pharmacokinetics. The PBPK model was successfully verified since all predictions were within twofold of observed clinical data. Peak concentration, area under the concentration‐time curve, and trough concentration were sensitive to media viscosity, drug solubility, drug density, and diffusion layer thickness. Additionally, inflammation was shown to impact the drug release from the depot. The developed framework correctly described the release and the drug disposition of LA formulations upon intramuscular administration. It can be implemented in PBPK models to address pharmacological questions related to the use of LA formulations.
Natural H2S‐donors: A new pharmacological opportunity for the management of overweight and obesity
Authors: Spezzini Jacopo , Piragine Eugenia , Flori Lorenzo , Calderone Vincenzo , Martelli Alma 
Published: March 2024

AbstractThe prevalence of overweight and obesity has progressively increased in the last few years, becoming a real threat to healthcare systems. To date, the clinical management of body weight gain is an unmet medical need, as there are few approved anti‐obesity drugs and most require an extensive monitoring and vigilance due to risk of adverse effects and poor patient adherence/persistence. Growing evidence has shown that the gasotransmitter hydrogen sulfide (H2S) and, therefore, H2S‐donors could have a central role in the prevention and treatment of overweight/obesity. The main natural sources of H2S‐donors are plants from the Alliaceae (garlic and onion), Brassicaceae (e.g., broccoli, cabbage, and wasabi), and Moringaceae botanical families. In particular, polysulfides and isothiocyanates, which slowly release H2S, derive from the hydrolysis of alliin from Alliaceae and glucosinolates from Brassicaceae/Moringaceae, respectively. In this review, we describe the emerging role of endogenous H2S in regulating adipose tissue function and the potential efficacy of natural H2S‐donors in animal models of overweight/obesity, with a final focus on the preliminary results from clinical trials. We conclude that organosulfur‐containing plants and their extracts could be used before or in combination with conventional anti‐obesity agents to improve treatment efficacy and reduce inflammation in obesogenic conditions. However, further high‐quality studies are needed to firmly establish their clinical efficacy.
Reduced‐order observer‐based finite‐time control for one‐sided Lipschitz nonlinear switched singular systems
Authors: Shi Hongpeng , Yang Anqing , Ma Shuping 
Published: March 2024

SummaryThe reduced‐order observer‐based finite‐time control problem for one‐sided Lipschitz nonlinear switched singular systems is addressed in this paper. First, the design method of the reduced‐order observer is given via state transformation. Then, based on the average dwell time (ADT) approach, some new sufficient conditions for regularity, impulse‐freeness, have a unique solution and finite‐time boundedness (FTB) of the dynamic augmented systems are obtained by exploring the reduced‐order observer‐based controller. Further, the lower finite‐time bound can be obtained by using singular value decomposition method. And the state feedback gain and the observer gain are computed by solving linear matrix inequalities (LMIs). Finally, the validity of the obtained method is illustrated by means of a numerical example and a DC motor system.
Organizational inclusion and sustainable development: An empirical evidence from the context of Eastern culture
Authors: Zeng Yanqin , Katsumata Sotaro , Zhang Qingyu , Li Xi 
Published: March 2024

AbstractThis study offers new insights into organizational inclusion and corporate sustainable development in the Japanese context. Specifically, we aim to explore organizational inclusion in the antecedents of Lesbian, Gay, Bisexual, and Transgender (LGBT) inclusion and its effects on corporate outcomes, such as corporate market performance (CMP) and corporate environmental performance (CEP). Drawing upon legitimacy theory, we propose a conceptual model to uncover how operational diversity encourages organizational inclusion, such as supportive policies and practices. Moreover, grounded in stakeholder theory, we explore how organizational inclusion affects corporate outcomes, including CMP and CEP. Using a large sample of 1456 Japan‐listed firms with detailed organizational inclusive practices and corporate outcomes. Results indicate that operational diversity has positive effects on organizational inclusion. Moreover, the results indicate that organizational inclusion strengthens CMP and CEP. The empirical results are consistent after performing robustness checks based on subsampling. Our study contributes to the global discussion on business ethics, corporate market responses, and sustainable development.
Testing the load capacity curve hypothesis with green innovation, green tax, green energy, and technological diffusion: A novel approach to Kyoto protocol
Authors: Degirmenci Tunahan , Aydin Mucahit 
Published: March 2024

AbstractEnvironmental degradation, which is seen as the most critical obstacle to sustainable development, is addressed by SDGs 7, 11, and 13. The Kyoto Protocol is the first international agreement to solve the problem of environmental degradation. The purpose of this research is to examine the role of Annex II nations in promoting environmental sustainability, particularly those that have adopted environmental obligations under the Kyoto Protocol. The study tests the load capacity curve hypothesis for Annex II countries during the 1994–2018 period. To begin with, we applied some priori tests to panel data analysis and utilized appropriate analysis methods based on the results. Unit root, cointegration, and long‐run estimators were employed. The findings confirmed a long‐run relationship for the load capacity curve model. In the end, two different estimators, namely cross‐sectional ARDL and augmented mean group estimator, were used to test the validity of the load capacity curve hypothesis. The study found that while the hypothesis did not hold true for all Annex II countries, it was only valid for Finland. This research suggests that Annex II countries are not adequately contributing to the achievement of the Kyoto Protocol targets.
The Multi-Factorial Modes of Action of Urease in the Pathogenesis of Incontinence Associated Dermatitis
Authors: Owen Emily J. , Heylen Rachel A. , Stewart Kyle , Winyard Paul G. , Jenkins Andrew Toby A. 
Published: March 2024

Abstract

Background
Incontinence Associated Dermatitis (IAD) is a type of skin inflammation caused by chronic exposure to urine and/or faeces. Current treatment strategies involve creating a barrier between the skin and urine/faeces rather than targeting specific irritants. Urease expressing pathogens catalyse the conversion of urea, present in urine, into ammonia. The accumulation of ammonia causes an elevation in skin pH which is believed to activate faecal enzymes which damage skin, and opportunistic pathogens, which lead to secondary infections.

Objectives
To develop a better, multi-factorial model of IAD pathogenesis, including the effect of urease-expressing bacteria on skin, mechanism of damage of urease and urease-triggered activity of faecal enzymes and secondary pathogens. To study the effect of urease inhibition on preventing IAD skin damage.

Methods
Five separate studies were made using ex vivo porcine skin and in vivo human skin models. Measurements of the change in skin barrier function were made using skin impedance, trans-epidermal water loss (TEWL), stratum corneum moisture and pH. Skin was exposed to artificial urine, inoculated with various microbes, enzymes and chemicals to examine the influence of: 1) urease-positive Proteus mirabilis 2) ammonia, 3) combination of P. mirabilis and a faecal enzyme, trypsin, 4) combination of P. mirabilis and opportunistic pathogens, Candida albicans and Staphylococcus aureus, 5) inhibition of urease using acetohydroxamic acid (AHA) on barrier function.

Results
The urease-mediated production of ammonia had two principal effects: it elevated skin pH and caused inflammation, leading to significant breakdown in skin (stratum corneum) barrier function. Urease was found to further increase the activity of faecal enzymes and opportunistic pathogens, due to elevated skin pH. The urease inhibitor, AHA, was shown to have significantly reduced damage to skin barrier function, measured as its electrical resistance.

Conclusions
Targeted therapeutic strategies should be developed to prevent the manifestation of IAD, rather than creating a generic barrier between skin and urine/faeces. Urease has been identified as a crucial component in the manifestation of IAD, due to its role in the production of ammonia. Urease inhibition provides a promising therapeutic target to halt the progression of IAD.
DFT Insights into FEBID Stability: Condensed Study of Gold(I) Complexes with Phosphine Ligands
Authors: Afaneh Akef T. , Alshhadat Mohanad , Marashdeh Ali , Almatarneh Mansour H. , Helal Wissam 
Published: March 2024

AbstractThis work examines the durability and responsiveness of XAuL precursors in the context of synthesizing Au‐nanomaterials. The study used density functional theory (DFT) simulations to look at the shapes, bond dissociation energies, and thermodynamic properties of a number of XAuL precursors. Within this framework, X denotes halogens Cl, Br, or I, whereas L denotes ligands such as PH3, PF3, PMe3, P(OMe)3, and P(NH2)3. Our investigations indicate that there is no discernible relationship between the lengths of XAuL bonds and binding energies. Small changes in bond dissociation free energy (▵BDG) serve as indicators of neutral precursors with low stability. The aforementioned precursors are essential for chemical vapor deposition. There are clear patterns that depend on the size of the halogen and the type of ligand when the ▵BDG values and interatomic distances in the XAuL precursors are looked at. Adding an electron to the precursor changes the length of the XAuL bond, which makes the ΔBDG values of anionic precursors lower than those of neutral precursors. The study is mostly about looking at how ligands L and XAu fragments interact with each other by looking at frontier molecular orbitals (FMOs). It has been shown that higher energy gap (ΔBDG) values lead to better interaction between the HOMO and LUMO orbitals of ligand L and XAu fragments. Furthermore, the research of electrophilicity emphasizes the significance of π‐back donation and the covalent energy involved in the bonding between XAu‐PR3. Thermodynamic study indicates that the dissociation processes exhibit more spontaneity under elevated temperatures and reduced pressures. The size of halogens affects spontaneity, with larger halogens exhibiting higher negative ▵BDG values. The study shows that IAuPMe3, BrAuPMe3, and ClAuPMe3 are stable starting materials for making gold nanoparticles using focused electron beam‐induced deposition (FEBID) in normal situations. This extensive study enhances our understanding of precursor design for Au‐nanofabrication by providing valuable insights into the stability, reactivity, and thermodynamic characteristics of XAuL precursors.
A Defective Disc‐Like Cu1.96S Anode Material with the Efficient Cu Vacancies for High‐Performance Sodium‐Ion Storage
Authors: Wang Zhihao , Lu Yongyi , Zhang Guangdi , Quan Lingfeng , Liu Mingzu , Liu Haimei , Wang Yonggang 
Published: March 2024

AbstractDue to their significant capacity and reliable reversibility, transition metal sulphides (TMSs) have received attention as potential anode materials for sodium‐ion batteries (SIBs). Nonetheless, a prevalent challenge with TMSs lies in their significant volume expansion and sluggish kinetics, impeding their capacity for rapid and enduring Na+ storage. Herein, a Cu1.96S@NC nanodisc material enriched with copper vacancies is synthesised via a hydrothermal and annealing procedure. Density functional theory (DFT) calculations reveal that the incorporation of copper vacancies significantly boosts electrical conductivity by reducing the energy barrier for ion diffusion, thereby promoting efficient electron/ion transport. Moreover, the presence of copper vacancies creates ample active sites for the integration of sodium ions, streamlines charge transfer, boosts electronic conductivity, and, ultimately, significantly enhances the overall performance of SIBs. This novel anode material, Cu1.96S@NC, demonstrates a reversible capacity of 339 mAh g−1 after 2000 cycles at a rate of 5 A g−1. In addition, it maintains a noteworthy reversible capacity of 314 mAh g−1 with an exceptional capacity retention of 96% even after 2000 cycles at 20 A g−1. The results demonstrate that creating cationic vacancies is a highly effective strategy for engineering anode materials with high capacity and rapid reactivity.
Heterostructured Graphene@Silica@Iron Phenylphosphinate for Fire‐Retardant, Strong, Thermally Conductive Yet Electrically Insulated Epoxy Nanocomposites
Authors: Chen Qiang , Huo Siqi , Lu Yixia , Ding Mingmei , Feng Jiabing , Huang Guobo , Xu Hang , Sun Ziqi , Wang Zhengzhou , Song Pingan 
Published: March 2024

AbstractThe portfolio of extraordinary fire retardancy, mechanical properties, dielectric/electric insulating performances, and thermal conductivity (λ) is essential for the practical applications of epoxy resin (EP) in high‐end industries. To date, it remains a great challenge to achieve such a performanceportfolio in EP due to their different and even mutually exclusive governing mechanisms. Herein, a multifunctional additive (G@SiO2@FeHP) is fabricated by in situ immobilization of silica (SiO2) and iron phenylphosphinate (FeHP) onto the graphene (G) surface. Benefiting from the synergistic effect of G, SiO2 and FeHP, the addition of 1.0 wt% G@SiO2@FeHP enables EP to achieve a vertical burning (UL‐94) V‐0 rating and a limiting oxygen index (LOI) of 30.5%. Besides, both heat release and smoke generation of as‐prepared EP nanocomposite are significantly suppressed due to the condensed‐phase function of G@SiO2@FeHP. Adding 1.0 wt% G@SiO2@FeHP also brings about 44.5%, 61.1%, and 42.3% enhancements in the tensile strength, tensile modulus, and impact strength of EP nanocomposite. Moreover, the EP nanocomposite exhibits well‐preserved dielectric and electric insulating properties and significantly enhanced λ. This work provides an integrated strategy for the development of multifunctional EP materials, thus facilitating their high‐performance applications.