bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of VEGF121/VEGF165–189 ratio results in a significant enhancement of human prostate tumor angiogenesis
Authors: Catena Raul , Muniz‐Medina Vanessa , Moralejo Beatriz , Javierre Biola , Best Carolyn J.M. , Emmert‐Buck Michael R. , Green Jeffrey E. , Baker Carl C. , Calvo Alfonso 
Published: February 2007

AbstractVascular endothelial growth factor (VEGF) is a proangiogenic factor upregulated in many tumors. The alternative splicing of VEGF mRNA renders 3 major isoforms of 121, 165 and 189 amino‐acids in humans (1 less amino‐acid for each mouse VEGF isoform). We have designed isoform specific real time QRT‐PCR assays to quantitate VEGF transcripts in mouse and human normal and malignant prostates. In the human normal prostate, VEGF165 was the predominant isoform (62.8% ± 5.2%), followed by VEGF121 (22.5% ± 6.3%) and VEGF189 (p < 0.001) (14.6% ± 2.1%). Prostate tumors showed a significant increase in the percentage of VEGF121 and decreases in VEGF165 (p < 0.01) and VEGF189 (p < 0.05). However, the amount of total VEGF mRNA was similar between normal and malignant prostates. VEGF164 was the transcript with the highest expression in the mouse normal prostate. Unlike human prostate cancer, tumors from TRAMP mice demonstrated a significant increase in total VEGF mRNA levels and in each of the VEGF isoforms, without changes in the relative isoform ratios. Morpholino phosphorodiamide antisense oligonucleotide technology was used to increase the relative amount of VEGF121 while proportionally decreasing VEGF165 and VEGF189 levels in human prostate cell lines, through the modification of alternative splicing, without changing transcription levels and total amount of VEGF. The increase in the VEGF121/VEGF165–189 ratio in PC3 cells resulted in a dramatic increase in prostate tumor angiogenesis in vivo. Our results underscore the importance of VEGF121 in human prostate carcinoma and demonstrate that the relative expression of the different VEGF isoforms has an impact on prostate carcinogenesis. © 2007 Wiley‐Liss, Inc.
Retracted: A modified version of galectin‐9 suppresses cell growth and induces apoptosis of human T‐cell leukemia virus type I‐infected T‐cell lines
Authors: Okudaira Taeko , Hirashima Mitsuomi , Ishikawa Chie , Makishi Shoko , Tomita Mariko , Matsuda Takehiro , Kawakami Hirochika , Taira Naoya , Ohshiro Kazuiku , Masuda Masato , Takasu Nobuyuki , Mori Naoki 
Published: February 2007

AbstractRetraction: The following article has been retracted through agreement between the first author and several coauthors, the journal Editor‐in‐Chief, Peter Lichter, and John Wiley & Sons, Ltd.: Okudaira T, Hirashima M, Ishikawa C, Makishi S, Tomita M, Matsuda T, Kawakami H, Taira N, Ohshiro K, Masuda M, Takasu N, Mori N. (2007). A modified version of galectin‐9 suppresses cell growth and induces apoptosis of human T‐cell leukemia virus type I‐infected T‐cell lines. Int J Cancer; 120 (May (10)): 2251‐2261, published online on 2 FEB 2007 in Wiley Online Library (www.onlinelibrary.wiley.com). After an investigation the retraction has been agreed due to inappropriate duplication of images and overlap with other published work.
Suppression of salivary adenoid cystic carcinoma growth and metastasis by ErbB3 binding protein Ebp1 gene transfer
Authors: Yu Youcheng , Chen Wantao , Zhang Yuexing , Hamburger Anne W. , Pan Hongya , Zhang Zhiyuan 
Published: February 2007

AbstractEbp1, an ErbB3 binding protein and downstream effector of the ErbB signaling network was shown to be a potent tumor suppressor in breast and prostate adenocarcinomas. We hypothesized that the inhibitory properties of the ebp1 gene could also be beneficial if ectopically expressed in salivary adenoid carcinoma. Salivary adenoid carcinoma cell line (ACC‐M) cells were stably transfected with the full‐length ebp1 cDNA sequence or the empty expression vector pcDNA3.1. Stable gene transfer was verified by Western blot analysis and reverse transcription (RT)‐PCR. A significant reduction in cell proliferation, anchorage‐independent growth, and a change in the cell cycle profile was observed in ebp1 transfectants. Matrigel assays demonstrated that the adenoid cystic carcinoma cell invasiveness was significantly reduced. A strong decrease in the metastatic potential of human adenoid cystic carcinoma cells in an experimental metastatic model was also observed. Our results suggest that ectopic expression of Ebp1 mediates multiple antitumor activities against adenoid cystic carcinoma cells and that ebp1 gene therapy might be a viable method suppressing malignant salivary adenoid tumors. © 2007 Wiley‐Liss, Inc.
A prospective study of dietary calcium, dairy products and prostate cancer risk (Finland)
Authors: Mitrou Panagiota N. , Albanes Demetrius , Weinstein Stephanie J. , Pietinen Pirjo , Taylor Philip R. , Virtamo Jarmo , Leitzmann Michael F. 
Published: February 2007

AbstractHigh dietary intakes of calcium and dairy products have been hypothesized to enhance prostate cancer risk, but available prospective data regarding these associations are inconsistent. We examined dietary intakes of calcium and dairy products in relation to risk of prostate cancer in the Alpha‐Tocopherol, Beta‐Carotene (ATBC) Cancer Prevention Study, a cohort of 29,133 male smokers aged 50–69 years at study entry. Dietary intake was assessed at baseline using a validated 276‐item food use questionnaire. Cox proportional hazards regression was used to adjust for known or suspected risk factors for prostate cancer. During 17 years of follow‐up, we ascertained 1,267 incident cases of prostate cancer. High versus low intake of dietary calcium was associated with a marked increase in prostate cancer risk. The multivariate relative risk (RR) of prostate cancer for ≥2,000 mg/day compared to <1,000 mg/day of calcium intake was 1.63 (95% confidence interval (CI), 1.27–2.10; p trend < 0.0001). Total dairy intake was also positively associated with risk of prostate cancer. The multivariate RR of prostate cancer comparing extreme quintiles of intake was 1.26 (95% CI, 1.04–1.51; p trend = 0.03). However, no association with total dairy intake remained after we adjusted for calcium (p trend = 0.17). Findings were similar by stage and grade of prostate cancer. The results from this large prospective study suggest that intake of calcium or some related component contained in dairy foods is associated with increased prostate cancer risk. © 2007 Wiley‐Liss, Inc.
Plasma C‐peptide, insulin‐like growth factor‐I, insulin‐like growth factor binding proteins and risk of colorectal cancer in a nested case‐control study: The Japan public health center‐based prospective study
Authors: Otani Tetsuya , Iwasaki Motoki , Sasazuki Shizuka , Inoue Manami , Tsugane Shoichiro 
Published: February 2007

AbstractThe physical inactivity and obesity involved in hyperglycemia and hyperinsulinemia is supposed to lead to an increased bioavailability of insulin‐like growth factor‐I (IGF‐I). The carcinogenic effect of IGF‐I may be influenced by IGF binding proteins. We investigated the association between plasma levels of C‐peptide, a surrogate biomarker of insulin, IGFBP‐1, IGF‐I or IGFBP‐3, and the risk of colorectal cancer in a nested case‐control study. During an 11.5‐year follow‐up, 375 newly diagnosed colorectal cancers were identified in a cohort of 38,373 adults who had returned the baseline questionnaire and provided blood samples. Two matched‐controls for each case were selected from the cohort. The odds ratio (OR) of colorectal cancer for plasma levels of each protein was estimated using the conditional logistic regression model adjusted for potential confounding factors. We observed a statistically significant association of plasma C‐peptide with colorectal cancer only in men. The ORs were 1.0, 2.3, 2.8 and 3.2 along with quartiles (p trend, 0.0072). The association was stronger in colon cancer (p trend, 0.025) than in rectal cancer (p trend, 0.24). Other peptides were not associated with the risk in either men or women. The results did not change when repeatedly analyzed by tumor invasion levels, tumor sites or follow‐up periods. In conclusion, a higher plasma C‐peptide may indicate a subsequent risk of colorectal cancer in Japanese men. © 2007 Wiley‐Liss, Inc.
Prosurvival function of the granulin‐epithelin precursor is important in tumor progression and chemoresponse
Authors: Pizarro Gresin O. , Zhou Xun Clare , Koch Anthony , Gharib Mahmood , Raval Sejal , Bible Keith , Jones Monica B. 
Published: February 2007

AbstractThe granulin‐epithelin precursor (GEP/PCDGF), a 68–88 kDa secreted glycoprotein, has been shown to be an important growth and survival factor for ovarian cancer cells. Furthermore, GEP expression is a predictor of patient survival in metastatic ovarian cancer cells. Up to this point, however, the molecular mechanisms and clinical relevance of a GEP‐mediated prosurvival phenotype remain poorly characterized. We hypothesize that the prosurvival function of GEP is important in ovarian cancer tumor progression and chemoresponse. To explore this hypothesis, we examined the effects of GEP overexpression on migration, invasion and cisplatin (CDDP) chemosensitivity in the ovarian cancer cell line A2780. Full length GEP transfectants demonstrated an increased capacity to migrate and invade their substratum when compared to empty vector controls. In addition, GEP overexpression was associated with CDDP chemoresistance. Finally, GEP overexpression increased tumor formation and protected cells from tumor regression in response to CDDP treatment in vivo. Taken together, these data support a role for GEP in tumor progression and development of drug resistance. © 2006 Wiley‐Liss, Inc.
Role of glutathione conjugation in the hepatotoxicity and immunotoxicity induced by 1‐bromopropane in female BALB/c mice
Authors: Lee Sang Kyu , Jeon Tae Won , Kim Yong Beom , Lee Eung Seok , Jeong Hye Gwang , Jeong Tae Cheon 
Published: February 2007

Abstract1‐Bromopropane (1‐BP) is used as a cleaning agent or adhesive solvent in the workplace. In the present study, the hepatotoxic and immunotoxic effects of 1‐bromopropane and its conjugation with glutathione (GSH) were investigated in female BALB/c mice. The animals were treated orally with 200, 500 and 1000 mg kg−1 of 1‐BP in corn oil for a dose response study or treated orally with 1000 mg kg−1 of 1‐BP for 6, 12, 24 and 48 h for a time course study. The hepatic and splenic contents of GSH were significantly decreased by 1‐BP in a dose‐dependent manner. S‐propyl GSH was identified in livers following treatment with 1‐BP by liquid chromatography‐electrospray ionization tandem mass spectrometry. When the production of conjugates from 1‐BP was investigated in livers following oral treatment with 1000 mg kg−1 of 1‐BP for 6, 12, 24 and 48 h, the GSH conjugates were detected maximally 6 h after treatment. Treatment of mice with 1‐BP increased the serum activity of alanine aminotransferase dose‐dependently. The oral 1‐BP treatment significantly suppressed the antibody response to a T‐dependent antigen and the production of splenic intracellular IL‐2 in response to Con A in a dose‐dependent manner. The present results suggested that 1‐BP could cause hepatotoxicity and immunotoxicity as well as depletion of GSH content due to the formation of GSH conjugates with 1‐BP in female BALB/c mice. Copyright © 2007 John Wiley & Sons, Ltd.
A sub‐acute study of metronidazole toxicity assessed in Egyptian Tilapia zillii
Authors: Khalil Wagdy K. B. , Mahmoud Mahmoud A. , Zahran Malak M. , Mahrous Karima F. 
Published: February 2007

AbstractMetronidazole (MTZ), an antiparasitic and antibacterial compound, is one of the world's most widely used drugs. Despite being considered as a rodent mutagen and a carcinogen, it is still widely used in humans for the treatment of infections with anaerobic organisms. Therefore, the main objective of the current study was to evaluate the in vivo toxicity of MTZ using the micronucleus (MN) assay and random amplified polymorphism DNA (RAPD‐PCR) analysis as well as histopathological examination in Tilapia zillii. Moreover, the protective effect of vitamin C (VitC) against toxicity of MTZ was investigated in the present study. Fish were treated with three doses of MTZ (5, 10 and 20 mg l−1) alone or in combination with VitC (200 mg kg−1 food) at several time intervals (2 days, 7 days and 14 days). The results of the present study showed a significant effect of MTZ on micronucleus formation and changes in polymorphic band patterns as well as induction of different histopathological alterations in Tilapia zillii. The effects of the drug were reduced when fish were exposed to a combination of MTZ and VitC. Copyright © 2007 John Wiley & Sons, Ltd.
Structure and strength at the bonding interface of a titanium‐segmented polyurethane composite through 3‐(trimethoxysilyl) propyl methacrylate for artificial organs
Authors: Sakamoto Harumi , Doi Hisashi , Kobayashi Equo , Yoneyama Takayuki , Suzuki Yoshiaki , Hanawa Takao 
Published: February 2007

AbstractThe objective of this study was to investigate the structure and strength at the bonding interface of a titanium (Ti)‐segmented polyurethane (SPU) composite through (3‐trimethoxysilyl) propyl methacrylate (γ‐MPS) for artificial organs. The effects of the thickness of the γ‐MPS layer on the shear bonding strength between Ti and SPU were investigated. Ti disks were immersed in various concentrations of γ‐MPS solutions for several immersion times. The depth profiles of elements and the thickness of the γ‐MPS layer were determined by glow discharge optical emission spectroscopy and ellipsometry, respectively. The bonding stress at the Ti/γ‐MPS/SPU interface was evaluated with a shear bonding test. Furthermore, the fractured surface of a Ti‐SPU composite was observed by optical microscopy and characterized using X‐ray photoelectron spectroscopy. Consequently, the thickness of the γ‐MPS layer was controlled by the concentration of the γ‐MPS solution and immersion time. The shear bonding stress at the interface increased with the increase of the thickness of the γ‐MPS layer. Therefore, the control of the thickness of the γ‐MPS layer is significant to increase the shear bonding stress at the Ti/γ‐MPS/SPU interface. These results are significant to create composites for artificial organs consisting of other metals and polymers. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver‐like structure by fetal liver cells in monolayer and three‐dimensional cultures
Authors: Ehashi Tomo , Koyama Toshie , Ookawa Keiko , Ohshima Norio , Miyoshi Hirotoshi 
Published: February 2007

AbstractVascular endothelial growth factor (VEGF) is crucial for the development and regeneration of the liver. However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and albumin secretion of FLCs, on VEGF secretion and morphological changes of long‐term cultured FLCs were investigated under three‐dimensional (3‐D) and monolayer conditions. The cultured FLCs proliferated well and showed stable secretion of VEGF for up to 1 month under both monolayer and 3‐D culture conditions. The addition of OSM to cultured cells strongly enhanced VEGF secretion. Compared with 3‐D cultures, VEGF secretion per cell was higher in monolayer cultures. After 1 month in culture, the FLCs in 3‐D cultures formed large aggregates like liver tissue, and FLCs also formed colonies and duct‐like structures after several months of culture even under monolayer conditions. In conclusion, OSM stimulated the secretion of VEGF by cultured FLCs, which seemed to contribute to the development of a liver‐like structure. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose
Authors: Křížová P. , Mášová L. , Suttnar J. , Salaj P. , Dyr J.E. , Homola J. , Pecka M. 
Published: February 2007

AbstractOxidized cellulose is an effective haemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma‐free medium or plasma‐free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet‐rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the haemostyptic effect of oxidized cellulose. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007
A physical vapor deposition method for controlled evaluation of biological response to biomaterial chemistry and topography
Authors: Hacking S.A. , Zuraw M. , Harvey E.J. , Tanzer M. , Krygier J.J. , Bobyn J.D. 
Published: February 2007

AbstractThe purpose of this study was to characterize a technique to effectively mask surface chemistry without modifying surface topography. A thin layer of titanium was deposited by physical vapor deposition (PVD) onto different biomaterial surfaces. Commercially pure titanium disks were equally divided into three groups. Disks were either polished to a mirror finish, grit blasted with alumina particles, or grit blasted and subsequently plasma sprayed with a commercial grade of hydroxyapatite (HA). A subgroup of each of these treatment types was further treated by masking the entire disk surface with a thin layer of commercially pure titanium deposited by PVD. A comparison of surface topography and chemical composition was carried out between disks within each treatment group. Canine marrow cells were seeded on all disk surfaces to determine the stability of the PVD Ti mask under culture conditions. The PVD process did not significantly alter the surface topography of any samples. The thin titanium layer completely masked the underlying chemistry of the plasma sprayed HA surface and the chemistry of the plasma vapor deposited titanium layer did not differ from that of the commercially pure titanium disks. Aliquots obtained from the media during culture did not indicate any significant differences in Ti concentration amongst the Ti and Ti‐masked surfaces. The PVD application of a Ti layer on HA coatings formed a stable, durable, and homogenous layer that effectively masked the underlying surface chemistry without altering the surface topography. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Bioactive surface for neural electrodes: Decreasing astrocyte proliferation via transforming growth factor‐β1
Authors: Klaver Christopher L. , Caplan Michael R. 
Published: February 2007

AbstractImplantation of deep‐brain recording devices is a traumatic event, which inevitably elicits reactive gliosis. The ensuing glial scar encapsulating the implanted device impedes the long‐term functional recording capability of the microelectrode. In this work, a bioactive surface is prepared by conjugation of transforming growth factor‐beta one (TGF‐β1) and laminin to dextran, which is in turn conjugated to a biomaterial substrate. Poly‐L‐lysine coated surfaces are treated with oxidized dextran, and the dextran is re‐oxidized with sodium metaperiodate to generate hemiacetal structures to which TGF‐β1 and laminin are covalently bound. Covalent conjugation of the ligand is confirmed by enzyme‐linked immunosorbent assay. A primary cell line of astrocytes is incubated on a surface conjugated with laminin and TGF‐β1 and a surface only conjugated with laminin. Proliferation on the laminin plus TGF‐β1 surface is 57% less (p < 0.002) than the control surface (laminin alone). The results demonstrate that conjugated TGF‐β1 retains its efficacy toward astrocyte proliferation and represents a potential strategy for reducing glial scar formation in vivo. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
A semiempirical free energy force field with charge‐based desolvation
Authors: Huey Ruth , Morris Garrett M. , Olson Arthur J. , Goodsell David S. 
Published: February 2007

AbstractThe authors describe the development and testing of a semiempirical free energy force field for use in AutoDock4 and similar grid‐based docking methods. The force field is based on a comprehensive thermodynamic model that allows incorporation of intramolecular energies into the predicted free energy of binding. It also incorporates a charge‐based method for evaluation of desolvation designed to use a typical set of atom types. The method has been calibrated on a set of 188 diverse protein–ligand complexes of known structure and binding energy, and tested on a set of 100 complexes of ligands with retroviral proteases. The force field shows improvement in redocking simulations over the previous AutoDock3 force field. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Prenatal diagnosis and management of fetal pharyngeal teratoma: A case report and review of the literature
Authors: Daskalakis George , Efthimiou Theodoros , Pilalis Athanasios , Papadopoulos Dimitrios , Anastasakis Eleftherios , Fotinos George , Antsaklis, Aris 
Published: February 2007

AbstractWe present a case of a fetal pharyngeal teratoma, which was diagnosed at 21 weeks' gestation. At the time of examination, a mass of mixed echogenicity was detected that protruded through the mouth. During a second examination 3 weeks later, the tumor had increased in size, and a severe polyhydramnios had developed. Intrauterine death of the fetus was detected at 27 weeks' gestation. Labor was induced with misoprostol, and a 1,015‐g stillborn female neonate was delivered. Postmortem examination confirmed the diagnosis of a pharyngeal teratoma. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2007