bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voltammetric analysis of pholcodine on graphene-modified GNPs/PTs with green assessment
Authors: Abdelshafi Nahla A. , Darwish Hany W. , Alanazi Ashwag S. , Naguib Ibrahim A. , Elkhouly Hadeer H. , Khodary Nehal S. , Mohamed Ekram H. 
Published: March 2024

AbstractPholcodine, an anti-tussive medication widely used as an over-the-counter, OTC drug, has recently faced restrictions in several countries. This paper presents a sensitive electrochemical approach for pholcodine detection. The electrochemical method involved fabricating a graphene nanoplatelets electrode, incorporating polythiophene nanospheres polymer to promote electron transfer and increase the activated surface area. Characterization of the fabricated electrode was performed using transmission electron microscopy, ATR-Fourier-transform infrared spectroscopy, X-ray crystallography, X-ray photoelectron spectroscopy, and electrochemical impedance spectroscopy. The electrochemical behavior of pholcodine with the fabricated electrode was investigated using cyclic voltammetry, chronoamperometry, square wave voltammetry (SWV), and differential pulse voltammetry (DPV). The developed electrode led to a linear response for pholcodine ranging from 10 to 45 mg/L with detection limits of 1.41 and 1.51 mg/mL for SWV and DPV, respectively and quantification limits of 4.27 and 4.57 mg/L for SWV and DPV, respectively. The proposed method has accurately recovered pholcodine in spiked serum samples with a recovery percentage ranging from 1.2 to 2.9%. The optimized method is found to be accurate, precise, and robust by applying validation parameters provided by International Council for Harmonization. Two green metrics were computed to assess the method’s greenness, the findings showed that the developed method is environmentally friendly with minimum sample preparation steps.
Hepatozoon (Eucoccidiorida: Hepatozoidae) in wild mammals of the Americas: a systematic review
Authors: Thomas Richard , Santodomingo Adriana , Saboya-Acosta Liliana , Quintero-Galvis Julian F. , Moreno Lucila , Uribe Juan E. , Muñoz-Leal Sebastián 
Published: March 2024

Abstract﻿
Background
The study of parasites provides insight into intricate ecological relationships in ecosystem dynamics, food web structures, and evolution on multiple scales. Hepatozoon (Eucoccidiorida: Hepatozoidae) is a genus of protozoan hemoparasites with heteroxenous life cycles that switch infections between vertebrates and blood-feeding invertebrates. The most comprehensive review of the genus was published 26 years ago, and currently there are no harmonized data on the epizootiology, diagnostics, genotyping methods, evolutionary relationships, and genetic diversity of Hepatozoon in the Americas.

Methods
Here, we provide a comprehensive review based on the PRISMA method regarding Hepatozoon in wild mammals within the American continent, in order to generate a framework for future research.

Results
11 out of the 35 countries of the Americas (31.4%) had data on Hepatozoon, with Carnivora and Rodentia orders having the most characterizations. Bats, ungulates, and shrews were the least affected groups. While Hepatozoon americanum, H. americanum-like, H. canis, H. didelphydis, H. felis, H. milleri, H. griseisciuri, and H. procyonis correspond to the identified species, a plethora of genospecies is pending for a formal description combining morphology and genetics. Most of the vectors of Hepatozoon in the Americas are unknown, but some flea, mite, and tick species have been confirmed. The detection of Hepatozoon has relied mostly on conventional polymerase chain reaction (PCR), and the implementation of specific real time PCR for the genus needs to be employed to improve its diagnosis in wild animals in the future. From a genetic perspective, the V4 region of the 18S rRNA gene has been widely sequenced for the identification of Hepatozoon in wild animals. However, mitochondrial and apicoplast markers should also be targeted to truly determine different species in the genus. A phylogenetic analysis of herein retrieved 18S ribosomal DNA (rDNA) sequences showed two main clades of Hepatozoon: Clade I associated with small mammals, birds, and herpetozoa, and Clade II associated with Carnivora. The topology of the tree is also reflected in the haplotype network.

Conclusions
Finally, our review emphasizes Hepatozoon as a potential disease agent in threatened wild mammals and the role of wild canids as spreaders of Hepatozoon infections in the Americas.

Graphical Abstract
Exploring the modulatory role of bovine lactoferrin on the microbiome and the immune response in healthy and Shiga toxin-producing E. coli challenged weaned piglets
Authors: Dierick Matthias , Ongena Ruben , Vanrompay Daisy , Devriendt Bert , Cox Eric 
Published: March 2024

Abstract
Background
Post-weaned piglets suffer from F18+Escherichia coli (E. coli) infections resulting in post-weaning diarrhoea or oedema disease. Frequently used management strategies, including colistin and zinc oxide, have contributed to the emergence and spread of antimicrobial resistance. Novel antimicrobials capable of directly interacting with pathogens and modulating the host immune responses are being investigated. Lactoferrin has shown promising results against porcine enterotoxigenic E. coli strains, both in vitro and in vivo.

Results
We investigated the influence of bovine lactoferrin (bLF) on the microbiome of healthy and infected weaned piglets. Additionally, we assessed whether bLF influenced the immune responses upon Shiga toxin-producing E. coli (STEC) infection. Therefore, 2 in vivo trials were conducted: a microbiome trial and a challenge infection trial, using an F18+ STEC strain. BLF did not affect the α- and β-diversity. However, bLF groups showed a higher relative abundance (RA) for the Actinobacteria phylum and the Bifidobacterium genus in the ileal mucosa. When analysing the immune response upon infection, the STEC group exhibited a significant increase in F18-specific IgG serum levels, whereas this response was absent in the bLF group.

Conclusion
Taken together, the oral administration of bLF did not have a notable impact on the α- and β-diversity of the gut microbiome in weaned piglets. Nevertheless, it did increase the RA of the Actinobacteria phylum and Bifidobacterium genus, which have previously been shown to play an important role in maintaining gut homeostasis. Furthermore, bLF administration during STEC infection resulted in the absence of F18-specific serum IgG responses.
MSCsDB: a database of single-cell transcriptomic profiles and in-depth comprehensive analyses of human mesenchymal stem cells
Authors: Yu Miao , Sui Ke , Wang Zheng , Zhang Xi 
Published: March 2024

AbstractMesenchymal stem cells (MSCs) possess multipotent properties that make them promising candidates for immunomodulation and regenerative medicine. However, MSC heterogeneity poses challenges to their research reproducibility and clinical application. The emergence of single-cell RNA sequencing (scRNA-seq) technology has enabled a thorough examination of MSC heterogeneity, underscoring the necessity for a specialized platform to systematically analyze the published datasets derived from MSC scRNA-seq experiments. However, large-scale integration and in-depth exploration of MSC scRNA-seq datasets to comprehensively depict their developmental patterns, relationships, and knowledge are still lacking. Here, we present MSCsDB (http://mscsdb.jflab.ac.cn:18088/index/), an interactive database that has been constructed using high-quality scRNA-seq datasets from all published sources on MSCs. MSCsDB provides a one-stop interactive query for regulon activities, gene ontology enrichment, signature gene visualization and transcription factor regulon analysis. Additionally, the dedicated module within MSCsDB was developed to facilitate the evaluation of MSC quality, thereby promoting the standardization of MSC subtype usage. Notably, MSCsDB enables users to analyze their MSCs scRNA-seq data directly, yielding visually appealing outputs of exceptional quality that can be conveniently downloaded via email. Furthermore, MSCsDB integrates the current comprehensive MSC atlas taxonomy, which includes 470,000 cells and 5 tissues from 26 subjects, as publicly available references. These references provide molecular characterization and phenotypic prediction for annotating MSC subsets. In summary, MSCsDB serves as a user-friendly and contemporary data repository for human MSCs, offering a dedicated platform that enables users to effectively conduct comprehensive analyses on their individual MSCs scRNA-seq data.
Attitude toward patients with mental disorders: what is going on amongst Iranian pharmacies?
Authors: Shirvaniyan Fatemeh , Eissazade Negin , Shariat Seved Vahid , Khademoreza Noushin , Daneshvar Masoomeh , Shalbafan Mohammadreza 
Published: March 2024

Abstract
Introduction
As the incidence of mental disorders continues to rise, and pharmacy staff can significantly affect the willingness of patients with mental disorders to seek help; we aimed to evaluate the stigmatizing attitude of the pharmacy staff toward these patients in Iran.

Methods
We conducted this cross-sectional study between April 2020 and December 2021 in Tehran, Iran, and included pharmacists, pharmacy technicians and pharmacy students, with the experience of working in a pharmacy for at least three months. The social distance scale (SDS) and dangerousness scale (DS) were used to measure the stigmatizing attitude of the participants. Higher scores indicated more stigmatizing attitudes.

Results
We included a total of 186 participants with a mean age of 32.97 ± 9.41 years, of which 101 (54.3%) were male, and 75 (40.3%) were pharmacists, 101 (54.3%) were pharmacy technicians, and 8 (4.3%) were pharmacy students. The mean SDS score was 14.2 ± 4.13, and the mean DS score was 33.85 ± 8.92. The greatest tendency for social distance was reported for a patient with a mental disorder, ‘being the caretaker of their children for an hour or two’ and ‘marrying their children.’ The most perceived dangerousness was reported for a patient with a mental disorder ‘owning a gun.’ Positive personal history of psychopharmacological treatment was statistically correlated with lower DS (P = 0.001) and SDS (P = 0.007) scores. Positive family history of psychiatric inpatient admission was significantly correlated with higher DS (P = 0.05) and SDS (P = 0.03) scores. Higher rates of ‘received psychiatric prescriptions per month’ was associated with lower DS scores (P = 0.04).

Conclusion
Our participants did not have an overall positive attitude toward patients with mental disorders. Although, compared to previous studies, they held a more positive attitude. Positive personal history of psychopharmacological treatment predicted a more positive attitude and positive family history of psychiatric inpatient admission predicted a more negative attitude.
Nurturing multiculturalism in Iranian EFL teacher education: an in-depth scrutiny of experts’ and teachers’ perceptions
Authors: Salimi Esmaeel Ali , Rad Mostafa Rahimi 
Published: March 2024

AbstractGiven that Iran is the host of a variety of cultures characterized with diverse languages, viewpoints, and customs, the empty place of multiculturalism in its ELT system is deeply felt. The present study sought to tackle multiculturalism in EFL teacher education in the context of Iran by exploring Iranian EFL experts’ and teachers’ perceptions. The participants of this study consisted of the two groups. The first group included 15 male and female multicultural education experts. The second group of the participants included of 60 (30 males and 30 females) Iranian EFL teachers. To collect the required data, a demographic information scale, two semi-structured interviews and an audio-reflective journal were used. Data analysis was done via qualitative thematic analysis by MAXQDA. As revealed by the results, several themes were extracted as the components of the multicultural EFL teacher education, as uncovered by the experts’ views some of which include Eliminating Race-Related Inequalities, Eliminating Gender-Related Inequalities, and Eliminating Social Class-Related Inequalities. Moreover, the Necessary Communicative Skills, Positive Thinking, Difference Acceptance, and Multicultural Content, Various Evaluation Methods were found as some of the extracted EFL teachers’ perceptions of multicultural EFL teacher education. The findings have some implications for different groups of stakeholders including EFL teachers, teacher educators and teacher education material developers who can take useful insights from the findings in teaching EFL, educating student-teachers and developing future curricula, respectively.
Dietary calcium is inversely associated with hepatitis B virus infection: an analysis of US National Health and Nutrition Examination Survey (NHANES) 2007–2020
Authors: Zhang Min , Zhang Yuxiao , Jiang Shanjiamei , Hu Heng , Wang Xinzhi , Yu Fan , Huang Yue’e , Liang Yali 
Published: March 2024

Abstract
Background
There have been studies on the relationship between hepatitis B virus (HBV) infection and diet. We hypothesized HBV infection is related to dietary calcium intake, but the evidence is limited. This study aimed to examine whether dietary calcium intake is independently related to HBV infection in the United States population.

Methods
A total of 20,488 participants aged over 20 years from the National Health and Nutrition Examination Survey (NHANES), conducted from 2007 to 2020, were included in this study. Pearson correlation was used to test the association between dietary calcium and serum calcium. The relationships of HBV infection with dietary calcium and serum calcium were assessed by logistic regression models.

Results
There was a weak correlation between dietary calcium and serum calcium (r = 0.048). Logistic regression models indicated that HBV infection had a linear negative correlation with dietary calcium (OR 0.37; 95%CI 0.19, 0.76). For each additional 10 mg dietary calcium, the possibility of HBV infection was reduced by 63%. Hepatitis B positive participants had lower serum calcium content than negative participants. Stratified analysis shown the linear relationship between calcium and HBV infection varied among sex, race/ethnicity, and body mass index.

Conclusion
Our findings demonstrated HBV infection was linearly and inversely correlated with dietary calcium. The current study is expected to offer a fresh perspective on reducing HBV infection.
Prevalence, characteristics and clinical impact of work-related musculoskeletal pain in echocardiography
Authors: Onwordi Eunice , Harris Alistair , Atkinson Charlotte , West Cathy , Pearce Keith , Hancock Jane , Demetrescu Camelia , Rakhit Dhrubo , Shah Benoy N. , Khattar Rajdeep , Gorman Jennifer , Encarnacion Delfin , Lloyd Guy , Bhattacharyya Sanjeev 
Published: March 2024

Abstract
Background
Work-related musculoskeletal pain (WRMSP) is increasingly recognised in cardiac ultrasound practice. WRMSP can impact workforce health, productivity and sustainability. We sought to investigate the prevalence, characteristics and clinical impact of WRMSP.

Methods
Prospective electronic survey of 157 echocardiographers in 10 institutions. Data acquired on demographics, experience, working environment/pattern, WRMSP location, severity and pattern, the impact on professional, personal life and career.

Results
129/157 (82%) echocardiographers completed the survey, of whom 109 (85%) reported WRMSP and 55 (43%) reported work taking longer due to WRMSP. 40/129 (31%) required time off work. 78/109 (60%) reported sleep disturbance with 26/78 (33%) of moderate or severe severity. 56/129 (45%) required medical evaluation of their WRMSP and 25/129 (19%) received a formal diagnosis of musculoskeletal injury. Those with 11+ years of experience were significantly more likely to receive a formal diagnosis of WRMSP (p = 0.002) and require medication (p = 0.006) compared to those with 10 years or less experience.

Conclusion
WRMSP is very common amongst echocardiographers, with a fifth having a related musculoskeletal injury. WRMSP has considerable on impact on personal, social and work-related activities. Strategies to reduce the burden of WRMSP are urgently required to ensure sustainability of the workforce and patient access to imaging.
Simplex Lattice Design and Machine Learning Methods for the Optimization of Novel Microemulsion Systems to Enhance p-Coumaric Acid Oral Bioavailability: In Vitro and In Vivo Studies
Authors: Nasser Nayera , Hathout Rania M. , Abd-Allah Hend , Sammour Omaima A. 
Published: March 2024

AbstractNovel p-coumaric acid microemulsion systems were developed to circumvent its absorption and bioavailability challenges. Simplex-lattice mixture design and machine learning methods were employed for optimization. Two optimized formulations were characterized using in vitro re-dispersibility and cytotoxicity on various tumor cell lines (MCF-7, CaCO2, and HepG2). The in vivo bioavailability profiles of the drug loaded in the two microemulsion systems and in the suspension form were compared. The optimized microemulsions composed of Labrafil M1944 CS (5.67%)/Tween 80 (38.71%)/Labrasol (38.71%)/water (16.92%) and Capryol 90 (0.50%)/Transcutol P (26.67%)/Tween 80 (26.67%)/Labrasol (26.67%)/water (19.50%), respectively. They revealed uniform and stable p-coumaric acid-loaded microemulsion systems with a droplet size diameter of about 10 nm. The loaded microemulsion formulations enhanced the drug re-dispersibility in contrast to the drug suspension which exhibited 5 min lag time. The loaded formulae were significantly more cytotoxic on all cell lines by 11.98–16.56 folds on MCF-7 and CaCo2 cells and 47.82–98.79 folds on HepG2 cells higher than the pure drug. The optimized microemulsions were 1.5–1.8 times more bioavailable than the drug suspension. The developed p-coumaric acid microemulsion systems could be considered a successful remedy for diverse types of cancer.
Graphical Abstract
Prospective Multicenter Registry–Based Study on Thyroid Storm: The Guidelines for Management From Japan Are Useful
Authors: Furukawa Yasushi , Tanaka Keiko , Isozaki Osamu , Suzuki Atsushi , Iburi Tadao , Tsuboi Kumiko , Iguchi Moritake , Kanamoto Naotetsu , Minamitani Kanshi , Wakino Shu , Satoh Tetsurou , Teramukai Satoshi , Kimura Eizen , Miyake Yoshihiro , Akamizu Takashi 
Published: March 2024

Abstract

Context
The mortality rate in thyroid storm (TS) has been reported to be higher than 10%.

Objective
We aimed to evaluate the effectiveness of the 2016 guidelines for the management of TS proposed by the Japan Thyroid Association and Japan Endocrine Society.

Methods
In this prospective multicenter registry–based study, patients with new-onset TS were registered in the Research Electronic Data Capture (REDCap), a secure web platform. On day 30 after admission, clinical information and prognosis of each patient were added to the platform. On day 180, the prognosis was described.

Results
This study included 110 patients with TS. The median of Acute Physiology and Chronic Health Evaluation (APACHE) II score was 13, higher than the score (10) in the previous nationwide epidemiological study (P = .001). Nonetheless, the mortality rate at day 30 was 5.5%, approximately half compared with 10.7% in the previous nationwide survey. Lower body mass index, shock, and lower left ventricular ejection fraction were positively associated with poor prognosis at day 30, while the lack of fever ≥ 38 °C was related to the outcome. The mortality rate in patients with an APACHE II score ≥ 12 for whom the guidelines were not followed was significantly higher than the rate in patients for whom the guidelines were followed (50% vs 4.7%) (P = .01).

Conclusion
Prognosis seemed better than in the previous nationwide survey, even though disease severity was higher. The mortality rate was lower when the guidelines were followed. Thus, the guidelines are useful for managing TS.
Visualization or Mathematization? The London School of Economics and “Diagrammatic Economics” in the 1930s
Authors: Giraud Yann 
Published: March 2024

Abstract
Although the subject of numerous contributions in the history of economics, the use of theoretical diagrams by economists is still quite misunderstood, as this practice is often characterized as a basic form of mathematization, soon replaced by more rigorous techniques of demonstration or exhibition. The present article attempts to challenge this view, focusing on a period of time, the 1930s, during which “diagrammatic economics” and other techniques coexisted, and on a specific community, that of students and young researchers from the London School of Economics with a keen interest in using diagrams as tools for economic reasoning. The cosmopolitan nature of research and education at the LSE, with its insistence on reading a vast amount of literature and attending seminars by a revolving cast of local and foreign lecturers, offered a favorable environment for a form of visual analysis that helped clarify concepts and resolve definitional issues, at a time when interest in various economic traditions had not yet given way to attempts to systematize and unify economic knowledge and its standards of production.
Applying Pure Mathematics: IMPA and the Entanglements of Mathematical Economics in Brazil
Authors: Assaf Matheus 
Published: March 2024

Abstract
This article studies the entanglements of mathematical economics in Brazil by delving into the emergence of a scientific community identified with the subject at the Instituto de Matemática Pura e Aplicada (IMPA). It traces how networks built since IMPA's early years in the 1950s made economics a viable alternative to applied mathematics in an institute mostly dedicated to research in pure mathematics. The article examines how interests in mathematical economics remained active during IMPA's expansion beginning in the late 1960s. This process culminated with the creation of the mathematical economics graduate program at IMPA in 1980 by Aloisio Araujo. The article follows how the program could build strong connections to the Escola de Pós-Graduação em Economia (EPGE), an important department of economics in Brazil, helping to shape recent developments in the center. The article thus aims to reveal how historical connections and entwinements of economics and mathematics groomed at IMPA have affected recent developments in the Brazilian economics community.
Sex-based differences in Muscle Stem Cell Regulation Following Exercise
Authors: Beaudry Kayleigh , De Lisio Michael 
Published: March 2024

Abstract
Sexual dimorphism, driven by the sex hormones testosterone and estrogen, influences body composition, muscle fiber type, and inflammation. Research related to muscle stem cell (MuSC) responses to exercise has mainly focused on males. We propose a novel hypothesis that there are sex-based differences in MuSC regulation following exercise, such that males have more MuSCs while females demonstrate a greater capacity for regeneration.