bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and Pharmacological Evaluation of New 1‐[3‐(4‐Arylpiperazin‐1‐yl)‐2‐hydroxypropyl]‐pyrrolidin‐2‐one Derivatives with Anti‐arrhythmic, Hypotensive, and α‐Adrenolytic Activity
Authors: Kulig Katarzyna , Sapa Jacek , Maciag Dorota , Filipek Barbara , Malawska Barbara 
Published: September 2007

AbstractA series of novel arylpiperazines bearing a pyrrolidin‐2‐one fragment was synthesized and evaluated for the binding affinity of the α1‐ and α2‐adrenoceptors (AR) and for the antiarrhythmic and hypotensive activities of the compounds. The most potent and selective compound 1‐[2‐hydroxy‐3‐[4‐[(2‐hydroxyphenyl)piperazin‐1‐yl]propyl]pyrrolidin‐2‐one 8 binds with pKi = 6.71 for α1‐AR. Derivative 8 was also the most active in the prophylactic antiarrhythmic test in adrenaline‐induced arrhythmia in anaesthetized rats. Its ED50 value equals 1.9 mg/kg (i.v.). Compounds with substituents such as a fluorine atom 4, a methyl 5, or a hydroxyl 8 group, or two substituents such as fluorine/chlorine atoms and methoxy groups in the phenyl ring, significantly decreased the systolic and diastolic pressure in normotensive anesthetized rats at a dosages of 5–10 mg/kg (i.v.). It was found that the presence of the piperazine ring and a hydroxy group in the second position of the propyl chain are critical structural features in determining the affinity of the compounds tested.
Pai syndrome: First patient with agenesis of the corpus callosum and literature review
Authors: Castori Marco , Rinaldi Rosanna , Bianchi Aurelia , Caponetti Aurelio , Assumma Marcello , Grammatico Paola 
Published: September 2007

AbstractBACKGROUND:Pai syndrome (PS) is a rare regional developmental defect of the face, mainly characterized by the variable association of midline cleft of the upper lip (MCL), duplicated maxillary median frenulum, and midline facial cutaneous and midanterior alveolar process polyps. Its entire clinical spectrum is still poorly delineated and the etiology remains unknown.CASE:We describe a 1‐month‐old boy presenting with MCL, left nostril hamartomatous mass, midline pedunculated polyp originating from the columella base, midline alveolar cleft, duplication of the upper median frenulum, unilateral persistent papillary membrane, lipoma of the corpus callosum, and additional minor facial dysmorphism. This patient also presents with agenesis of the corpus callosum, which has never been reported in PS. Literature review was carried out comparing clinical data of the 20 previously published patients with those observed in the present case.CONCLUSIONS:The minimum diagnostic criteria for PS has been fixed in one or more hamartomatous nasal polyps plus MCL (with or without cleft alveolus) and/or midanterior alveolar process congenital polyp. Additional common ancillary findings include duplicated median maxillary frenulum, hypertelorism, nasal cleft, midfrontal skin tags, and ocular and CNS structural abnormalities. However, mental retardation is only an occasional feature and seems to be related to coexisting conditions (such as chromosome imbalance). Literature review shows that PS is etiologically heterogeneous, as it may result from chromosome abnormalities and environmental/stochastic events, as well as de novo mutations. Birth Defects Research (Part A) 2007. © 2007 Wiley‐Liss, Inc.
Aplasia cutis congenita with skull defect in a monozygotic twin after exposure to methimazole in utero
Authors: Iwayama Hideyuki , Hosono Haruki , Yamamoto Hikaru , Oshiro Makoto , Ueda Norishi 
Published: September 2007

AbstractBACKGROUND:Aplasia cutis congenita (ACC) is a condition in which localized or widespread areas of skin are absent at birth. Defective lesions show complete absence of all layers of skin, occasionally extending to skull or dura. ACC is etiologically heterogeneous; many different etiologies including teratogens have been documented.CASE:We describe the first reported case of a monozygotic twin with ACC after exposure to methimazole in utero. The female patient was born at 36 weeks gestation as the first child of monozygotic twins. The mother received methimazole between the 11th and 17th weeks of pregnancy because of transient hyperthyroidism. The second child did not have ACC. The patient had defects of the scalp, skull, and dura (7 × 5 cm) on the sagittal line of the parieto‐occipital region. No other malformations were noted. The scalp defect has been treated daily with sterile physiological saline and petrolatum dressing in addition to intravenous antibiotics. Trafermin, a recombinant human fibroblast growth factor, was sprayed from day 6 to promote epithelialization of the scalp defect. On day 21, she had high fever due to infection of the defect lesion, which was controlled by povidone iodine dressing and intravenous antibiotics. The defect of the scalp was well healed after 6 weeks, but the skull defect remained unclosed.CONCLUSIONS:We describe a rare case of a monozygotic twin with ACC and skull defect after methimazole exposure in utero. The findings of our case suggest that methimazole is a potential teratogen of ACC. Birth Defects Research (Part A) 2007. © 2007 Wiley‐Liss, Inc.
Precursor‐directed biosynthesis of stilbene methyl ethers in Escherichia coli
Authors: Katsuyama Yohei , Funa Nobutaka , Horinouchi Sueharu 
Published: September 2007

AbstractStilbenes are bioactive compounds that show beneficial effects for humans, such as anti‐tumor activity and survival improvement. Resveratrol, a representative of stilbenes and showing various health‐improving activities, is rapidly metabolized in humans, and modified resveratrols are therefore desired as anti‐cancer drugs and dietary polyphenols. An Escherichia coli system, in which an artificial stilbene biosynthetic pathway, including steps of phenylalanine ammonia‐lyase, 4‐coumarate:CoA ligase, and stilbene synthase, was reconstructed, produced stilbenes in high yields: resveratrol from tyrosine and pinosylvin from phenylalanine. To incorporate a stilbene methyltransferase gene into this E. coli system, cDNA of Os08g06100 in Oryza sativa was expressed and its O‐methylating activity toward stilbenes was confirmed. Incorporation of the pinosylvin methyltransferase (OsPMT) gene into the pathway established in E. coli led to production of mono‐ and di‐methylated stilbenes. Furthermore, the OsPMT gene turned out to be useful in production of unnatural stilbene methyl ethers due to its rather relaxed substrate specificity; various carboxylic acids supplemented as precursors, such as p‐fluorocinnamic acid, 3‐(2‐furyl)acrylic acid, 3‐(2‐thienyl)acrylic acid, and 3‐(3‐pyridyl)acrylic acid, to the E. coli system carrying the steps of 4‐coumarate:CoA ligase, stilbene synthase, and OsPMT were converted to stilbene dimethyl ethers with the corresponding carboxylic moiety.
A novel antibiotic free plasmid selection system: Advances in safe and efficient DNA therapy
Authors: Mairhofer Jürgen , Pfaffenzeller Irene , Merz David , Grabherr Reingard 
Published: September 2007

AbstractThe presence of antibiotic resistance genes in the delivered plasmids is one of the drawbacks of modern gene therapy and DNA vaccine applications. Here, we describe a strategy that allows for plasmid selection in bacterial hosts, without the requirement of any selection marker. Several bacterial strains were modified, so that the plasmid's replicational inhibitor RNA I could suppress the translation of a growth essential gene by RNA‐RNA antisense reaction. An essential gene (murA) was modified such that a repressor protein (tetR) would hamper its expression. Only in the presence of plasmid and, hence, RNA I, was tetR turned down and murA expressed. Different commercially available plasmids could be selected by various modified Escherichia coli strains. We further designed a minimalistic plasmid devoid of any selection marker. All of the clones (n=6) examined, when the modified strain JM109‐murselect was used for selection, contained plasmids. Thus, we have designed bacterial host strains that for the first time serve to select and maintain plasmids without the use of any selection marker or other additional sequence on the plasmid. Consequently, such plasmids may not only be safer, but due to their decreased size, advantages for the manufacturer and higher transfection efficiencies are anticipated.
Structure–function relationships in RNA and RNP enzymes: Recent advances
Authors: Hoogstraten Charles G. , Sumita Minako 
Published: September 2007

AbstractThe structural biology of ribozymes and ribonucleoprotein (RNP) enzymes is now sufficiently advanced that a true dialogue between structural and functional studies is possible. In this review, we consider three important systems in which an integration of structural and biochemical data has recently led to major advances in mechanistic understanding. In the hammerhead ribozyme, application‐driven biochemical studies led to the discovery of a key structural interaction that had been omitted from previously‐studied constructs. A new crystal structure of the resulting, tertiary‐stabilized hammerhead has resolved a remarkable number of longstanding paradoxes in the structure–function relationship of this ribozyme. In the Group I intron ribozyme, a flurry of high‐resolution structures has largely confirmed, but in some cases refined or challenged, a detailed model of a metalloenzyme active site that had previously been derived by meticulous quantitative metal ion rescue experiments. Finally, for the peptidyl transferase center of the ribosome, recent biochemical and chemical results motivated by the pioneering crystal structures have suggested a picture of a catalytic mechanism dominated by proximity and orientation effects and substrate‐assisted catalysis. These results refocus attention on catalysis as a property of the integrated RNP machinery as a whole, as opposed to a narrow concern with the RNA functional groups in immediate contact with the reactive center. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 317–328, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Evidence for the 310‐helical structure of peptides based on antAib, a fluorophoric, anthracene‐fused, 1‐aminocyclopentane‐1‐carboxylic acid
Authors: Wright Karen , Lohier Jean‐François , Wakselman Michel , Mazaleyrat Jean‐Paul , Peggion Cristina , Formaggio Fernando , Toniolo Claudio 
Published: September 2007

AbstractPeptides based on 2‐amino‐2,3‐dihydro‐1H‐cyclopenta[b]anthracene‐2‐carboxylic acid (antAib), a fluorescent, achiral, α‐amino acid belonging to the class of C→C cyclized, Cα,α‐disubstituted glycines, combined with L‐Ala, up to the hexamer level, were synthesized by solution methods and chemically characterized. A conformational analysis by FTIR absorption and NMR techniques suggests that the highest oligomers of this series tend to fold into β‐turns/310‐helices. The UV absorption, CD, and fluorescence properties of these antAib/L‐Ala model peptides are also described. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 88: 797–806, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Demonstration of REBASE‐assisted restriction mapping to determine the recognition site of unknown restriction endonucleases
Authors: Parashar Vijay , Capalash Neena , Sharma Prince 
Published: September 2007

AbstractAn important step in the characterization of a new restriction enzyme involves determination of its recognition site. Comparison of its DNA substrate digestion fragment patterns with those obtained using enzymes of known specificity indicates whether the enzyme recognizes a novel sequence or is an isoschizomer of already existing prototype. REBASE (Restriction Enzyme dataBASE: http://www.neb.com/rebase)‐assisted restriction mapping is described in this paper for a rare cutter [TspMI (REBASE No. 7191)] and a frequent cutter [BflI (REBASE No. 4910)] as a practical exercise for undergraduate students to understand how to determine recognition sequence of a REase.
Gel electrophoresis
Authors: Tan Timothy Ter Ming , Tan Zong Ying , Tan Wei Liang , Lee Peng Foo Peter 
Published: September 2007

AbstractThe aim of this project is to develop a simple system for the teaching and demonstration of DNA gel electrophoresis. DNA gel electrophoresis requires the use of specialized apparatus, toxic reagents, expensive agarose gel, and DNA samples, as well as a considerable amount of valuable classroom time to complete. A systematic evaluation of suitable alternative materials and components for the simulation of DNA gel electrophoresis was undertaken. A tried and tested set of combinations is presented here for educators to use in hands‐on classroom teaching, which does not require DNA, agarose, or Tris–borate–EDTA buffer. The use of common biological stains in place of DNA samples, agar–agar‐based gels, and weak electrolyte solutions provides a simple, inexpensive, and highly reproducible system that is adaptable to instructional needs. The migration of multicolored bands during electrophoresis provides an intuitive, compelling demonstration of the concept of electrophoresis.
Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods
Authors: Suwanjinda Duongdearn , Eames Chris , Panbangred Watanalai 
Published: September 2007

AbstractWe describe a practical laboratory designed for third‐year undergraduate students of Biotechnology as part of a Microbial Physiology and Genetics course. It comprises a five‐session laboratory module to screen foods for lactic acid bacteria (LAB)1 and to test isolated LAB for the presence of bacteriocins. Traditional Thai fermented foods are first screened for bacteriocin‐producing LAB using microbiological methods. This is followed by a simple and rapid DNA extraction and by a multiplex polymerase chain reaction (PCR) using three pairs of specific primers to test for the presence or absence of various bacteriocin genes in the isolated LAB. PCR amplicons of 332, 412, and 608 bp indicate the presence of pediocin, enterocin, and nisin genes, respectively, whereas no amplicon band indicates the absence of these bacteriocins. The laboratory provides the students with experience in the use of microbiological and multiplex PCR methods and shows how the molecular biology techniques can be related to their daily lives. The module could easily be adapted to the study of fermented foods from other countries.
Teaching lactose metabolism
Authors: Monti‐Hughes Andrea , Alonso Manuel , Garófalo Judith , Burgos Hilda Isabel , Stella Carlos Alberto 
Published: September 2007

AbstractWe developed an experimental didactic proposal to teach both carbohydrate metabolism and lactose intolerance as the disease related to that metabolism. Therefore, we implemented an empirical strategy consisting of inexpensive and nontoxic components for which students do not need to know any of the laboratory techniques. The fact that students were able to discuss their own results obtained from the experiments performed in their classroom gave them an additional motivation to learn the subject.
Blackboard electrophoresis
Authors: Costa M. J. 
Published: September 2007

AbstractUndergraduates with little training on molecular biology may find the technical level of the typical introductory restriction laboratory too challenging and have problems with mastering the underlying concepts and processes. Blackboard electrophoresis is an active learning exercise, which focuses student attention on the sequences and principles of DNA restriction. Students convert short strings of letters of A, C, G, and Ts into blackboard electrophoresis profiles analogous to gel electrophoresis of restriction digests. Students work in teams to i) invent short strings of letters representing polynucleotides; ii) identify and count the number of specific restriction sequences in each string; iii) fractionate the strings in restriction fragments and count their size; and iv) predict blackboard electrophoretic patterns in which fragments are represented by sizes. The exercise is inexpensive, since it does not require laboratory equipment or supplies and accommodates a plethora of introductory contexts that can be explored to bring in relevance to students. The exercise has been used with high school and university audiences with very positive outcomes. Blackboard electrophoresis is a valuable complement or alternative to other instructional approaches to teach restriction analysis and DNA diversity.
Investigation of the properties of wild type and mutant alkaline phosphatase variants: A laboratory project linking genotype and phenotype
Authors: Howitt Susan M. 
Published: September 2007

AbstractAn understanding of the link between genotype and phenotype is essential for biology students. A 3‐wk laboratory project aimed at demonstrating this link and introducing early year students to some aspects of the research process is described. Students investigate the properties of wild type and mutant variants of alkaline phosphatase using the techniques of both biochemistry and molecular biology. Changes in enzyme activity are correlated with the changes in DNA sequence that introduce restriction enzyme sites. Mutants are also used to analyze the regulation of phoA gene expression. The application of different techniques to the same experimental system helps students to integrate information from different parts of the course.
Light‐Up Hoechst–DNA Aptamer Pair: Generation of an Aptamer‐Selective Fluorophore from a Conventional DNA‐Staining Dye
Authors: Sando Shinsuke , Narita Atsushi , Aoyama Yasuhiro 
Published: September 2007

AbstractWe have designed a strategy to generate a light‐up fluorophore–aptamer pair based on a down‐modification of a conventional DNA‐staining dye to suppress its affinity to the original dsDNA targets, followed by reselection of aptamers that would bind to the modified dye. Following this line, we prepared a micropolarity‐sensitive Hoechst derivative possessing two tBu groups with low affinity to the usual AT‐rich dsDNA targets. DNA aptamers selected in vitro from a random pool worked as triggers to enhance the fluorescence of an otherwise nonfluorescent Hoechst derivative, and the shortened 25‐mer sequence showed remarkable enhancement (light‐up). The 25‐mer sequence was split into binary aptamer probes, thus enabling us to detect a target nucleic acid sequence with a single‐nucleotide resolution by use of unmodified DNA as a probe.
Crystal Structure of a New Compound (Sr0.47Ca0.53)2Al.
Authors: Zhang Q. A. , Yang W. M. , Enoki H. , Akiba E. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
New Iodate Materials as Potential Laser Matrices. Preparation and Characterization of α‐Ln(IO3)3 (Ln: Y, Dy) and β‐Ln(IO3)3 (Ln: Y, Ce, Pr, Nd, Eu, Gd, Tb, Dy, Ho, Er). Structural Evolution as a Function of the Ln3+ Cationic Radius.
Authors: Phanon Delphine , Mosset Alain , Gautier‐Luneau Isabelle 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
η‐Y2Si2O7 — Structural Investigations on a Quenchable High‐Pressure Mixed Anion Silicate.
Authors: Kahlenberg V. , Kaindl R. , Konzett J. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Crystal Structure of the New Ternary Compound Ce3Ru2In3.
Authors: Kurenbaeva Zh. M. , Tursina A. I. , Murashova E. V. , Nesterenko S. N. , Gribanov A. V. , Seropegin Yu. D. , Noel H. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Magnetic Disorder in the Frustrated Antiferromagnet Jarosite Arising from the H3O+×××OH‐ Interaction.
Authors: Grohol Daniel , Nocera Daniel G. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis, Crystal Structures, Metal‐Atom Distribution and Magnetic Properties of Cr5‐xTixSe8 (x ≈ 2, 3, 4).
Authors: Wontcheu J. , Kockelmann W. , Huang Zhong‐Le , Schnelle W. , Bensch W. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Ab initio Molecular Dynamics Study of the Hydrolysis Reaction of Diborane.
Authors: Di Pietro Elisa , Cardini Gianni , Schettino Vincenzo 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Preparation and Characterization of M2O3—CeO2 (M: B, Al, Ga, In) Mixed‐Oxide Catalysts.
Authors: Yuzhakova T. , Rakic V. , Guimon C. , Auroux A. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Self‐Propagating High‐Temperature Synthesis of Sr‐Doped LaMnO3 Perovskite as Oxidation Catalyst.
Authors: Hirano T. , Purwanto H. , Watanabe T. , Akiyama T. 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of Metal—(Pentadentate‐Salen) Complexes: Asymmetric Epoxidation with Aqueous Hydrogen Peroxide and Asymmetric Cyclopropanation (salenH2: N,N′‐Bis(salicylidene)ethylene‐1,2‐diamine).
Authors: Shitama Hiroaki , Katsuki Tsutomu 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Cinchona Alkaloid‐Catalyzed Enantioselective Monofluoromethylation Reaction Based on Fluorobis(phenylsulfonyl)methane Chemistry Combined with a Mannich‐Type Reaction.
Authors: Mizuta Satoshi , Shibata Norio , Goto Yosuke , Furukawa Tatsuya , Nakamura Shuichi , Toru Takeshi 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Catalytic Asymmetric Phase‐Transfer Michael Reaction and Mannich‐Type Reaction of Glycine Schiff Bases with Tartrate‐Derived Diammonium Salts.
Authors: Shibuguchi Tomoyuki , Mihara Hisashi , Kuramochi Akiyoshi , Ohshima Takashi , Shibasaki Masakatsu 
Published: September 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.