bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intrahepatic Cholestasis Occurring With Prurigo of Pregnancy
Authors: Cicek Demet , Kandi Basak , Demir Betul , Turgut Dilara 
Published: November 2007

A 23‐year‐old woman presented to our polyclinic complaining of itching, generalized dermatitis, and jaundice. She was in her 31st gestational week and had developed pruritus and the dermatitis since the first month of pregnancy; her jaundice started about a month before presentation. Her history included similar complaints in a previous pregnancy, which resulted in premature birth of a baby with a permanent brain defect. One of her sisters had had jaundice and itching in her 27th gestational week and delivered a healthy baby; a second sister had experienced itching and dermatitis in her second trimester and delivered a healthy baby. Physical examination of the patient showed that her eyes were jaundiced (Figure 1); skin examination revealed generalized erythematous excoriated papules, symmetrically distributed all over her body (Figure 2Figure 3). Laboratory analyses revealed the following results: leukocyte count, 14.30/mm3 (3.8–10.3/mm3); erythrocyte sedimentation rate, 25 mm/h (<20 mm/h); aspartate aminotransferase, 44 U/L (5–40 U/L); alanine aminotransferase, 63 U/L (5–40 U/L); lactate dehydrogenase, 1158 U/L (220–450 U/L); total bilirubin, 6.88 mg/dL (<1.10 mg/dL); and direct bilirubin, 3.27 mg/dL (<0.35 mg/dL). Urinalysis results were positive for bilirubin and urobilinogen. Positive serologic findings included rubella immunoglobulin G, 93 AU/mL (<15) and cytomegalovirus, 188 AU/mL (<10); negative findings included herpes simplex virus type 2 and hepatitis. Histopathologic examination of material collected from the left breast via punch biopsy showed parakeratosis, acanthosis, and perivascular lymphocyte infiltration in dermal vessels. Treatment with 2 g/d cholestyramine and a topical corticosteroid was effective in the patient, who was diagnosed with intrahepatic cholestasis of pregnancy and prurigo of pregnancy based on the clinical, histopathologic, and laboratory findings. To the authors' knowledge, this is the first such reported case in the literature. Scleral icterus. image Multiple symmetric excoriated erythematous papules on trunk and extensor extremities. image Excoriated erythematous papules on legs. image
Scrofuloderma: A Forgotten Disease?
Authors: Kiliç Arzu , Gül Ülker , Soylu Seçil , Kaya Ïlhan , Albayrak Levent 
Published: November 2007

A 30‐year‐old woman was evaluated in our clinic with swelling localized to the left submandibular region and extending to the anterior part of the neck. Her symptoms first appeared 2 years earlier in the mid region of her neck. After a few months, the lesion enlarged to the left side of her neck and became crusted. She had been treated by several physicians with antibiotics, analgesics, and anti‐inflammatory drugs without response. On physical examination, the patient had an axillary temperature of 38.2°C. Bilateral axillary, cervical, and supraclavicular lymph nodes were detected. Dermatologic examination revealed erythematous nodules of varying sizes starting at the middle of the neck and extending to the left cervical region; an ulcerated lesion on the mid portion of the neck; and a sinus tract in the left cervical region (Figure 1). Laboratory findings included an erythrocyte sedimentation rate of 62 mm/h, but no irregularities were detected in the other hematologic and biochemical parameters. The results were negative for hepatitis markers, human immunodeficiency virus, and syphilis. A purified protein derivative test revealed an 18‐mm induration with a scar of bacilli Calmette‐Guérin at 48 hours. Results of chest radiography were normal, but a computerized tomographic scan of the lung revealed emphysematous changes. Acid‐fast bacilli examination of the sputum was performed 3 times, all with negative results, and sputum cultures were negative for tuberculosis and deep fungal infections. Results of polymerase chain reaction testing of skin tissue, sputum, and urine were negative as well. Abdominal and pelvic ultrasonographic finding were normal; no tuberculous focus was detected from detailed examinations of pulmonary, genitourinary, and skeletal systems. Staining of a biopsy specimen obtained from lesions in the cervical region was positive for acid‐fast bacilli, and cultures were positive for Mycobacterium tuberculosis. The histopathologic features of the biopsy specimen included caseation necrosis at the center and epithelioid histiocytes, lymphocytes, plasma cells, and Langhans cells at the periphery of the granulomas (Figure 2). These findings confirmed the diagnosis of scrofuloderma. The patient received multiagent (isoniazid, rifampicin, ethambutol, and pyrazinamide) antituberculosis treatment for 3 months. The treatment was continued with isoniazid, rifampicin, and ethambutol for 1 year, with follow‐up clinic visits throughout the period. The lesions improved within the first 3 months of treatment (Figure 3). The ulcerated lesion on the middle and left side of the neck before multiagent antituberculosis treatment. image A close‐up view showing caseation necrosis, granulomatous inflammatory cells, epithelioid tubercles, and Langhans cells (hematoxylin and eosin stain; original magnification ×40). image The appearance of the lesions after 3 months of treatment. image
The effects of acute intraocular pressure elevation on rat retinal glutamate transport
Authors: Holcombe David J. , Lengefeld Nadia , Gole Glen A. , Barnett Nigel L. 
Published: November 2007

Abstract.Purpose:  To investigate the relationship between intraocular pressure (IOP), retinal glutamate transport and retinal hypoxia during acute IOP elevations of varying magnitude.Methods:  Female Dark Agouti rats were anaesthetized by ketamine/xylazine/acepromazine (10/5/0.5 mg/kg i.p.). The anterior chamber was cannulated with a 30‐gauge needle attached to a saline reservoir. The target IOP (20–120 mmHg, in 10 mmHg increments) was obtained by adjusting the reservoir height. After 10 mins of IOP stabilization, 2 μl of the non‐endogenous glutamate transporter substrate, D‐aspartate, was injected into the vitreous (final concentration 50 μm), and the elevated IOP maintained for a further 60 mins (total duration of IOP elevation was 70 mins). Glutamate transporter function was assessed by the immunohistochemical localization of D‐aspartate. Retinal sections were examined for histological integrity. The experiment was repeated substituting the D‐aspartate with the cellular hypoxia marker, Hypoxyprobe‐1.Results:  Under control conditions, D‐aspartate was preferentially taken up into the glial Müller cells by glutamate/aspartate transporter (GLAST). This function was maintained at pressures ≤ 70 mmHg, whereafter perturbation of function was evidenced by decreased accumulation of D‐aspartate by Müller cells. Failure of GLAST activity was coincident with the appearance of Hypoxyprobe‐labelled cells in the inner retina and histological damage.Conclusions:  Glutamate transport does not appear to change linearly with increased IOP. A pressure threshold exists, above which Müller cell GLAST function is compromised. Moreover, ganglion cell glutamate uptake is only apparent at pressures above those that cause GLAST inhibition. The association between IOP, hypoxia, glutamate transporter dysfunction and subsequent retinal cell death may have important implications for the pathogenesis of IOP/ischaemia‐related neuropathy and neuroprotective strategies.
Reduction in admissions of patients with acute primary angle closure occurring in conjunction with a rise in cataract surgery in Taiwan
Authors: Hu Chao‐Chien , Lin Herng‐Ching , Chen Chin‐Shyan , Kuo Nai‐Wen 
Published: November 2007

Abstract.Purpose:  Using 8 year nationwide administrative data, this study sets out to investigate the relationship between the total number of cataract operations undertaken in Taiwan and admissions for acute primary angle closure (APAC).Methods:  Monthly cataract surgery and APAC admission rates, per 100 000 of the population, were provided by 1997–2004 inpatient and outpatient data obtained from the Taiwanese National Health Insurance Research Database. The 3814 cases of APAC and 503 687 patients who had undergone cataract operations were categorized by age groups (40–49, 50–59, 60–69 and ≥70 years) and by gender. Spearman rank correlation coefficients were used to examine the direction and strength of the relationships.Results:  Throughout the study period, the admissions for APAC showed a steady decline from 630 cases in 1997 to 351 cases in 2004, while the number of cataract operations revealed a gradual increase from 26 600 in 1997 to 77 924 in 2004. The Spearman rank correlation coefficients showed significant inverse relationships between monthly APAC admission rates and monthly cataract operation rates for the total group (r = −0.407, P < 0.001), males (r = −0.330, P < 0.001), females (r = −0.444, P < 0.001), 40–49 year olds (r = −0.335, P < 0.001), 50–59 year olds (r = −0.497, P < 0.001) and 60–69 year olds (r = −0.417, P < 0.001). No significant inverse relationship was observed for the ≥70 age group.Conclusion:  Significant inverse relationships were noted between the monthly APAC admission rates and the monthly cataract operation rates. We recommend that data should be collected from other regions and ethnic groups to determine the inter‐relationships.
Complications in patients after intravitreal injection of bevacizumab
Authors: Shima Chiharu , Sakaguchi Hirokazu , Gomi Fumi , Kamei Motohiro , Ikuno Yasushi , Oshima Yusuke , Sawa Miki , Tsujikawa Motokazu , Kusaka Shunji , Tano Yasuo 
Published: November 2007

Abstract.Purpose:  To report complications in patients after intravitreal injection of bevacizumab to treat ocular diseases associated with vascular endothelial growth factor.Methods:  We retrospectively reviewed the systemic and ocular complications that developed within 2 months of each intravitreal injection of bevacizumab in 707 patients (1300 injections) with intraocular neovascularization or macular oedema.Results:  Nine ocular (1.27%) and eight systemic (1.13%) complications occurred in 707 patients. The ocular complications included corneal abrasion (n = 2), chemosis (n = 2), lens injury (n = 1), ocular inflammation (n = 2), retinal pigment epithelial tear (n = 1) and acute vision loss (n = 1). The systemic complications included cerebral infarction (n = 1), elevation of systolic blood pressure (n = 2), facial skin redness (n = 1), itchy diffuse rash (n = 1) and menstrual irregularities (n = 3).Conclusion:  Intravitreal injection of bevacizumab may cause systemic or ocular complications. Caution is advised when considering intravitreal injection of this drug.
Dissociative symptoms are positively related to stress in borderline personality disorder
Authors: Stiglmayr C. E. , Ebner‐Priemer U. W. , Bretz J. , Behm R. , Mohse M. , Lammers C.‐H. , Anghelescu I.‐G. , Schmahl C. , Schlotz W. , Kleindienst N. , Bohus M. 
Published: November 2007

Objective:  According to DSM‐IV criteria, dissociative symptoms in borderline personality disorder (BPD) occur in response to stress. Empirical evidence is, however, lacking.Method:  Using ambulatory monitoring, we assessed dissociative symptoms and subjective ratings of stress every 60 min for 48 h on a palmtop computer in BPD‐patients (n = 51), clinical controls (CC; major depression n = 25; panic disorder n = 26), and healthy controls (HC; n = 40). Data analyses were primarily based on hierarchical linear models.Results:  In all groups, states of increased stress were paralleled by increased scores of dissociation, thus confirming the hypothesized association between stress and dissociation. The increase in dissociation was more pronounced in BPD‐patients when compared with CC and HC. Additionally, BPD‐patients reported heightened dissociative experience compared with CC and HC, even after controlling for stress.Conclusion:  Our data suggest that BPD‐patients might be prone to dissociation when experiencing stress and are characterized by a generally heightened level of dissociation.
Evaluation of the prognostic relevance of l‐selectin and ICAM1 expression in myelodysplastic syndromes
Authors: Buccisano Francesco , Maurillo Luca , Tamburini Anna , Del Poeta Giovanni , Del Principe Maria Ilaria , Ammatuna Emanuele , Consalvo Maria Irno , Campagna Selenia , Ottaviani Licia , Sarlo Chiara , Renzi Daniela , Faccia Sabrina , Fraboni Daniela , Coco Francesco Lo , Amadori Sergio , Venditti Adriano 
Published: November 2007

AbstractObjectives:  An aberrant pattern of expression of l‐selectin and intercellular adhesion molecule 1 (ICAM1) may characterise CD34+ blast cells in myelodysplastic syndromes (MDS) and secondary acute myeloid leukaemia (sAML).Methods:  In a three‐colour flow cytometric assay, we evaluated the expression of l‐selectin and ICAM1 on CD34+ blast cells from the bone marrow (BM) of 66 MDS patients; for the purpose of comparison CD34+ blast cells of 18 sAML and CD34+ stem cells of 17 normal donors were also analysed.Results:  The ratio of l‐selectin/ICAM1 expression was identified as a parameter correlated with the percentage of BM blast infiltration and the time to leukaemic progression among MDS patients. In fact, the values of l‐selectin/ICAM1 ratio were inversely correlated with the BM blast infiltration (r = –0.34, P = 0.004). Furthermore, MDS patients with a baseline ratio <1 had a higher leukaemic progression rate (41% vs. 19%, P = 0.008); the actuarial risk of disease progression for this subgroup of MDS patients was also higher (64% vs. 11% at 2 yr, P = 0.002). Furthermore, in two patients a decrease of the ratio was observed when overt leukaemic transformation occurred; conversely, restoration of a normal ratio was observed in two patients after a chemotherapy‐induced remission.Conclusion:  (i) l‐selectin is defective in the stem cell compartment of MDS and sAML, whereas ICAM1 is overexpressed; (ii) the ratio of their expression has a prognostic role; and (iii) a ratio <1 significantly predicts progression to overt leukaemia in MDS patients.
Effects of fluquinconazole and silthiofam, applied as seed treatments to single or consecutive crops of wheat, on take‐all epidemic development and grain yields
Authors: Bateman G.L. , Gutteridge R.J. , Jenkyn J.F. , Self M.M. 
Published: November 2007

AbstractSeed treatments containing fluquinconazole, silthiofam or a standard fungicide mixture with no activity against take‐all were compared in all combinations of sequences in successive second and third winter wheat crops in five field experiments and second to fourth crops in a sixth experiment. Compared with the standard treatment, silthiofam decreased take‐all more effectively than fluquinconazole when crops were sampled at tillering. In samples taken in summer, during grain filling, silthiofam often decreased the incidence of take‐all (percentage of plants with root symptoms) more than fluquinconazole, but fluquinconazole more effectively decreased the incidence of severe take‐all (percentage of plants with more than 75% of their root systems blackened). It is suggested that these differences are a consequence of more effective control of primary infection of roots by silthiofam and of secondary, root‐to‐root, infection by fluquinconazole. Silthiofam usually increased yield more than did fluquinconazole, perhaps as a consequence of better early protection during tiller and/or spikelet formation. Treatment with either of the fungicides affected epidemic development in the treated crop and in crops grown subsequently. In particular, decreased take‐all had the effect of delaying the year‐to‐year epidemic, so that nontreatment of a subsequent crop resulted in an upsurge in disease. Treatment with either take‐all fungicide of a crop grown after a treated crop was relatively effective if the epidemic in the comparable nontreated crop sequence was continuing to increase. It was, however, detrimental if the disease was approaching its peak in the first treated crop, particularly if a treated (fourth wheat) crop was being compared with a similar crop in a nontreated sequence in which take‐all decline had developed. These results provide a basis for recommendations for the use of seed treatment fungicides in sequences of wheat crops.
Variability of responses to 1‐methylcyclopropene by banana: influence of time of year at harvest and fruit position in the bunch
Authors: Moradinezhad F. , Sedgley M. , Klieber A. , Able A.J. 
Published: November 2007

AbstractTo examine the effect of early‐climacteric (postripening) 1‐methylcyclopropene (1‐MCP) exposure on the shelf‐life and quality of green Cavendish bananas (Musa acuminata cv. Williams) from the middle section of the bunch, bananas were harvested bimonthly and treated with 100 μL L−1 ethylene for 2 consecutive days prior to exposure to 0, 100, 300, 1000, 3000 or 10 000 nL L−1 1‐MCP for 24 h prior to storage at 22°C. 1‐MCP treatment at a concentration of 300 nL L−1 or above increased banana shelf‐life significantly compared with the control, regardless of the month in which fruit were harvested except March where a higher concentration was needed (3000 nL L−1). Fruit harvested in May were the most responsive with a greater than twofold increase in shelf‐life. To examine the effect of fruit position in the bunch on 1‐MCP efficacy, green fruit from the top or bottom of bunches were treated with 100 μL L−1 ethylene for 2 consecutive days prior to early‐climacteric 1‐MCP (300 nL L−1) exposure for 24 h at 22°C. In spring and autumn but not in summer, application of 1‐MCP to early‐climacteric fruit was more effective in fruit from the top than in those treated from the bottom of the bunch, increasing shelf‐life. Firmness of 1‐MCP‐treated fruit was up to 19% greater than that of the control across the year, except in fruit from the bottom of the bunch. Given that 1‐MCP is less effective in extending the shelf‐life of summer‐harvested fruit (particularly those from the bottom of the bunch), we conclude that preharvest conditions and fruit position in the bunch affect their responsiveness to ethylene and their behaviour during the ripening process.
Growth Performance and Proximate and Fatty Acid Compositions of Channel Catfish, Ictalurus punctatus, Fed for Different Duration with a Commercial Diet Supplemented with Various Levels of Menhaden Fish Oil
Authors: Yildirim‐Aksoy Mediha , Shelby Richard , Lim Chhorn , Klesius Phillip H. 
Published: November 2007

Abstract A 15‐wk study was conducted to evaluate the effect of supplemental menhaden fish oil levels and feeding duration on growth performance and tissue proximate and fatty acid (FA) compositions of juvenile channel catfish, Ictalurus punctatus. Dietary fish oil levels had no effect on final weight gain, feed efficiency, and survival of channel catfish. Tissue lipid contents were directly correlated to dietary lipid levels, while moisture contents were inversely related to dietary lipid levels. Fillet moisture contents progressively decreased, whereas fillet lipid increased with increasing feeding duration. Significant increase in saturated and total n‐3 FAs and decrease in monoenoic and total n‐6 FA in whole body and fillet were observed at each incremental level of dietary fish oil. Percentages of n‐3 and n‐3 highly unsaturated fatty acids in fillet of fish fed the control and 3% fish oil diets decreased with increasing feeding periods, whereas those of fish fed 6 or 9% added fish oil diets remained stable or increased. Ratios of n‐3/n‐6 were statistically comparable throughout the 15‐wk feeding. When expressed in terms of mg/g of fillet, the highest concentration of n‐3 was obtained in fillets of fish fed the 9% added fish oil diet for 15 wk.
Intergeneric Hybridization between Kutum, Rutilus frisii kutum, and Bream, Abramis brama orientalis, of the Caspian Sea
Authors: Amini F. , Zamini A. A. , Ahmadi M. R. 
Published: November 2007

Abstract Artificial hybridization was performed between Rutilus frisii kutum and Abramis brama orientalis of the Caspian Sea. Synchronization of spawning of female broodstock of both species was induced by injection of carp pituitary extract. Reciprocal crossings between R. frisii kutum ♀×A. brama orientalis ♂ (RA) and A. brama orientalis ♀×R. frisii kutum ♂ (AR) produced viable hybrid larvae without any clear particular pre‐ or postzygotic isolation phenomena. RA and AR hybrid larvae were reared to fingerling stage with survival rates of 22.5 and 28% and average weight of 6.8 ± 0.17 g and 9.0 ± 0.79 g, respectively. A heterosis of 45% was calculated for weight at fingerling stage. RA and AR hybrid fingerlings were cultured in polyculture along with Chinese carps for 6–7 mo and reached an average weight of 190–195 g and 235–255 g, respectively. Karyotyping of these hybrids revealed a modal diploid number of 2n = 50 for both groups, which is similar to those of the parental species. Discriminant function analysis on 28 morphometric and meristic characteristics of two parental species as well as their hybrids could separate these groups at highly significant level (P < 0.001). These results indicated an overall intermediate inheritance of the studied characters.
Identification of Immunologic and Pathologic Parameters of Death versus Survival in Respiratory Tularemia
Authors: Chiavolini Damiana , Alroy Joseph , King Carol A. , Jorth Peter , Weir Susan , Madico Guillermo , Murphy John R. , Wetzler Lee M. 
Published: November 2007

ABSTRACTFrancisella tularensiscan cause severe disseminated disease after respiratory infection. The identification of factors involved in mortality or recovery following induction of tularemia in the mouse will improve our understanding of the natural history of this disease and facilitate future evaluation of vaccine candidate preparations. BALB/c mice were infected intranasally with the live vaccine strain (LVS) ofF. tularensissubsp.holarcticaand euthanized at different stages of disease to analyze the induction of immune molecules, gross anatomical features of organs, bacterial burdens, and progression of the histopathological changes in lung and spleen. Tissue-specific interleukin-6 (IL-6), macrophage inflammatory protein 2, and monocyte chemotactic protein 1 were immune markers of mortality, while anti-LVS immunoglobulin M and IL-1β were associated with survival. Moribund mice had enlarged spleens and lungs, while surviving mice had even more prominent splenomegaly and normal-appearing lungs. Histopathology of the spleens of severely ill mice was characterized by disrupted lymphoid follicles and fragmented nuclei, while the spleens of survivors appeared healthy but with increased numbers of megakaryocytes and erythrocytes. Histopathology of the lungs of severely ill mice indicated severe pneumonia. Lungs of survivors at early time points showed increased inflammation, while at late times they appeared healthy with peribronchial lymphoid aggregates. Our results suggest that host immune factors are able to affect bacterial dissemination after respiratory tularemia, provide new insights regarding the pathological characteristics of pulmonary tularemia leading to systemic disease, and potentially identify immune markers associated with recovery from the disease.