bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surfactant therapy for acute respiratory failure in children: a systematic review and meta-analysis
Authors: Duffett Mark , Choong Karen , Ng Vivian , Randolph Adrienne , Cook Deborah J 
Published: June 2007

Abstract
Introduction
Exogenous surfactant is used to treat acute respiratory failure in children, although the benefits and harms in this setting are not clear. The objective of the present systematic review is to assess the effect of exogenous pulmonary surfactant on all-cause mortality in children mechanically ventilated for acute respiratory failure.

Methods
We searched the MEDLINE, EMBASE, CINAHL and Ovid Healthstar databases, the bibliographies of included trials and review articles, conference proceedings and trial registries. We included prospective, randomized, controlled trials of pulmonary surfactant that enrolled intubated and mechanically ventilated children with acute respiratory failure. We excluded trials that exclusively enrolled neonates or patients with asthma. Two reviewers independently rated trials for inclusion, extracted data and assessed the methodologic quality. We quantitatively pooled the results of trials, where suitable, using a random effects model.

Results
Six trials randomizing 314 patients were included. Surfactant use reduced mortality (relative risk = 0.7, 95% confidence interval = 0.4 to 0.97, P = 0.04), was associated with increased ventilator-free days (weighted mean difference = 2.5 days, 95% confidence interval = 0.3 to 4.6 days, P = 0.02) and reduced the duration of ventilation (weighted mean difference = 2.3 days, 95% confidence interval = 0.1 to 4.4 days, P = 0.04).

Conclusion
Surfactant use decreased mortality, was associated with more ventilator-free days and reduced the duration of ventilation. No serious adverse events were reported.
Detailed analysis of 15q11-q14 sequence corrects errors and gaps in the public access sequence to fully reveal large segmental duplications at breakpoints for Prader-Willi, Angelman, and inv dup(15) syndromes
Authors: Makoff Andrew J , Flomen Rachel H 
Published: June 2007

Abstract
Background
Chromosome 15 contains many segmental duplications, including some at 15q11-q13 that appear to be responsible for the deletions that cause Prader-Willi and Angelman syndromes and for other genomic disorders. The current version of the human genome sequence is incomplete, with seven gaps in the proximal region of 15q, some of which are flanked by duplicated sequence. We have investigated this region by conducting a detailed examination of the sequenced genomic clones in the public database, focusing on clones from the RP11 library that originates from one individual.

Results
Our analysis has revealed assembly errors, including contig NT_078094 being in the wrong orientation, and has enabled most of the gaps between contigs to be closed. We have constructed a map in which segmental duplications are no longer interrupted by gaps and which together reveals a complex region. There are two pairs of large direct repeats that are located in regions consistent with the two classes of deletions associated with Prader-Willi and Angelman syndromes. There are also large inverted repeats that account for the formation of the observed supernumerary marker chromosomes containing two copies of the proximal end of 15q and associated with autism spectrum disorders when involving duplications of maternal origin (inv dup[15] syndrome).

Conclusion
We have produced a segmental map of 15q11-q14 that reveals several large direct and inverted repeats that are incompletely and inaccurately represented on the current human genome sequence. Some of these repeats are clearly responsible for deletions and duplications in known genomic disorders, whereas some may increase susceptibility to other disorders.
Acute alcohol activates STAT3, AP-1, and Sp-1 transcription factors via the family of Src kinases to promote IL-10 production in human monocytes
Authors: Norkina Oxana , Dolganiuc Angela , Shapiro Taryn , Kodys Karen , Mandrekar Pranoti , Szabo Gyongyi 
Published: June 2007

AbstractAlcohol consumption is associated with an imbalance in pro- and anti-inflammatory cytokines and immunosuppression, partially as a result of enhanced IL-10 production. The mechanisms of IL-10 induction by alcohol remain poorly understood. We identified that increased IL-10 production in human monocytes after acute in vivo alcohol consumption or in vitro alcohol treatment was associated with increased STAT3 activation. Alcohol alone induced and in combination with LPS augmented STAT3 phosphorylation at tyrosine 705 (tyr705) and serine 727 (ser727) residues and increased STAT3 binding to DNA. Upstream, alcohol activated the Src kinases, as indicated by an increase in phosphorylated and a decrease in nonphosphorylated Src proteins. STAT3 activation by Src kinases occurred directly at the tyr705 residue and indirectly at the ser727 residue via JNK MAPKs. Using specific Src (PP2), JNK1/2 (SB600125), or p38 (SB203580) inhibitors, we determined that alcohol treatment alone induced and together with LPS, augmented the DNA-binding capacity of the specificity protein-1 (Sp-1) and AP-1 transcription factors involved in IL-10 production via Src-mediated activation of p38 MAPK and JNK, respectively. Our data suggest that acute alcohol activates Src/STAT3 and Src/MAPK/STAT3, AP-1, and Sp-1 pathways as important mechanisms for IL-10-mediated immunomodulation after acute alcohol use.
Rapid T cell receptor-mediated SHP-1 S591 phosphorylation regulates SHP-1 cellular localization and phosphatase activity
Authors: Liu Yin , Kruhlak Michael J , Hao Jian-Jiang , Shaw Stephen 
Published: June 2007

AbstractSince the tyrosine phosphatase SHP-1 plays a major role in regulting T cell signaling, we investigated regulation thereof by Ser/Thr phosphorylation. We found that T cell receptor (TCR) stimulation induced fast (≤1 min) and transient phosphorylation of SHP-1 S591 in both Jurkat and human peripheral blood T-cells (PBT). Phosphorylation of S591 in T-cells could be mediated artificially by a constitutive active PKC-theta construct, but the dose dependence of inhibition by PKC inhibitors indicated that PKCs were not the relevant basophilic kinase in the physiological response. S591 phosphorylation inhibited phosphatase function since a S591D mutant had lower activity than the S591A mutant. Additional evidence that S591 phosphorylation alters SHP-1 function was provided by studies of Jurkat cells stably expressing SHP-1 wild type or mutants. In those cells, S591D mutation reduced the capacity of transfected SHP-1 to inhibit TCR-induced phosphorylation of PLC-γ1. Interestingly, SHP-1 Y536 phosphorylation (previously shown to augment phosphatase activity) was also induced in PBT by TCR signal but at a much later time compared with S591 (∼30 min). S591 phosphorylation also altered cellular distribution of SHP-1 because: 1) SHP-1 in lipid rafts and a sheared membrane fraction was hypophosphorylated; 2) In stably transfected Jurkat cell lines, S591D mutant protein had reduced presence in both lipid raft and the sheared membrane fraction; 3) S591 phosphorylation prevented nuclear localization of a C-terminal GFP tagged SHP-1 construct. Our studies also shed light on an additional mechanism regulating SHP-1 nuclear localization, namely conformational autoinhibition. These findings highlight elegant regulation of SHP-1 by sequential phosphorylation of serine then tyrosine.
Oxygen-sensitive regulatory volume increase and Na transport in red blood cells from the cane toad,Bufo marinus
Authors: Kristensen Karina , Koldkjær Pia , Berenbrink Michael , Wang Tobias 
Published: June 2007

SUMMARYThe red blood cells (RBCs) of cane toad, Bufo marinus, are only partially saturated with oxygen in most of the circulation due to cardiac shunts that cause desaturation of arterial blood. The present study examines the oxygen dependency of RBC ouabain-insensitive unidirectional Na transport,using 22Na, in control cells and in cells exposed to hyperosmotic shrinkage or the β-adrenergic agonist isoproterenol. Deoxygenation per se induced a slow, but significant Na influx, which was paralleled by a slow increase in RBC volume. Hyperosmotic shrinkage by a calculated 25% activated a robust Na influx that in the first 30 min had a strong PO2 dependency with maximal activation at low PO2 values and a P50of ∼5.5 kPa. This activation was completely abolished by the Na/H exchanger (NHE) inhibitor EIPA (10–4 mol l-1). Hyperosmotic shrinkage is particularly interesting in B. marinus as it withstands considerable elevation in extracellular osmolarity following dehydration. Parallel studies showed that deoxygenated B. marinusRBCs had a much faster regulatory volume increase (RVI) response than air-equilibrated RBCs, reflecting the difference in magnitude of Na influxes at the two PO2 values. The extent of RVI(∼60%) after 90 min, however, was similar under the two conditions,reflecting a more prolonged elevation of the shrinkage-induced Na influx in air-equilibrated RBCs. There were no significant differences in the ability to perform RVI between whole blood cells at a PCO2of 1 and 3 kPa or washed RBCs, and 10–4 mol l-1amiloride reduced the RVI under all conditions, whereas 10–5mol l-1 bumetanide had no effect. Isoproterenol(10–5 mol l-1) induced a significant and prolonged increase in an EIPA-sensitive and bumetanide-insensitive Na influx at low PO2 under iso-osmotic conditions, whilst there was no stimulation by isoproterenol for up to 45 min in air-equilibrated RBCs. The prolonged β-adrenergic activation of the Na influx at low PO2 is distinctly different from the rapid and transient stimulation in teleost RBCs, suggesting significant differences in the signal transduction pathways leading to transporter activation between vertebrate groups.
CoCl2 induces protective eventsviathe p38-MAPK signalling pathway and ANP in the perfused amphibian heart
Authors: Gaitanaki Catherine , Kalpachidou Theodora , Aggeli Ioanna-Katerina S. , Papazafiri Panagiota , Beis Isidoros 
Published: June 2007

SUMMARYMitogen-activated protein kinases (MAPKs) constitute one of the most important intracellular signalling pathways. In particular, the p38-MAPK subfamily is known to be activated under various stressful conditions, such as mechanical or oxidative stress. Furthermore, cobalt chloride(CoCl2) has been shown to mimic hypoxic responses in various cell lines and cause overproduction of reactive oxygen species (ROS). In the current study, we investigated the effect of CoCl2 on p38-MAPK signalling pathway in the perfused Rana ridibunda heart. Immunoblot analysis of the phosphorylated, and thus activated, form of p38-MAPK revealed that maximum phosphorylation was attained at 500 μmol l-1CoCl2. A similar profile was observed for MAPKAPK2 and Hsp27 phosphorylation (direct and indirect p38-MAPK substrates, respectively). Time course analysis of p38-MAPK phosphorylation pattern showed that the kinase reached its peak within 15 min of treatment with 500 μmol l-1CoCl2. Similar results were obtained for Hsp27 phosphorylation. In the presence of the antioxidants Trolox or Lipoic acid, p38-MAPK CoCl2-induced phosphorylation was attenuated. Analogous results were obtained for Hsp27 and MAPKAPK2. In parallel, mRNA levels of the ANP gene, a hormone whose transcriptional regulation has previously been shown to be regulated by p38-MAPK, were examined (semi-quantitative ratiometric RT-PCR). CoCl2 treatment significantly increased ANP mRNA levels,whereas, in the presence of antioxidants, the transcript levels returned to basal values. All the above data indicate that CoCl2 stimulates compensatory mechanisms involving the p38-MAPK signalling cascade along with ANP.
Electrical and behavioral courtship displays in the mormyrid fish Brienomyrus brachyistius
Authors: Wong Ryan Y. , Hopkins Carl D. 
Published: June 2007

SUMMARY
Mormyrid electric fish rely on the waveform of their electric organ discharges (EODs) for communicating species, sex, and social status, while they use the sequences of pulse intervals (SPIs) for communicating rapidly changing behavioral states and motivation. Little is known of electric signaling during courtship behavior because of two major difficulties: (1) the fish are not easily bred in captivity and (2) there is no reliable means of separating electric signals from several individuals in natural communication settings. Through simulating artificial rain conditions, we have successfully induced courtship and succeeded in breeding a mormyrid electric fish(Brienomyrus brachyistius) in the laboratory. We have also developed a system of video recording and editing combined with cross correlation analysis to precisely record and view behavior and separate EODs from two individuals in non-breeding and breeding contexts. Knowing the electrical and motor patterns during courtship allows for further exploration of topics such as mate choice and neural basis of pattern generation in these fish.
Here we describe nine common motor displays and 11 SPIs. Analysis of frequency of occurrences suggests that some SPI patterns are sex and season specific. We also observed electrical duetting called `rasp matching' during courtship signaling among pairs; males and females exchange `rasps' and`bursts', respectively, in alternation. Our study employs new techniques to separate and document SPIs in the context of courtship. We show that some SPIs correlate with specific behavioral acts around the time of spawning.
Relationship between avian growth rate and immune response depends on food availability
Authors: Brzęk Pawel , Konarzewski Marek 
Published: June 2007

SUMMARYLife history theory predicts that when resources are limited growing organisms are likely to trade an immune response for competing demands of growth. To test this we examined the effect of energy intake on body mass increments and an immune response in hand-reared sand martin (Riparia riparia) nestlings. We subjected the nestlings to three different feeding regimes, mimicking the range of food availability in the wild, and then evaluated nestlings' immune response to phytohaemagglutinin (PHA). Direction of correlation between the magnitude of PHA-induced swelling response and body mass increments depended on food availability: it was negative when food was scarce and positive when resources were plentiful. There was no significant correlation between the two traits under intermediate feeding conditions. We conclude that the relative cost of immune function in young birds depends on food availability and, therefore, may be modified by external factors such as weather conditions or hatching asynchrony.
Mechanosensation and mechanical load modulate the locomotory gait of swimmingC. elegans
Authors: Korta Jeremie , Clark Damon A. , Gabel Christopher V. , Mahadevan L. , Samuel Aravinthan D. T. 
Published: June 2007

SUMMARYAnimals move through their environments by selecting gaits that are adapted to the physical nature of their surroundings. The nematode Caenorhabditis elegans swims through fluids or crawls on surfaces by propagating flexural waves along its slender body and offers a unique opportunity for detailed analysis of locomotory gait at multiple levels including kinematics,biomechanics and the molecular and physiological operation of sensory and motor systems. Here, we study the swimming gait of C. elegans in viscous fluids in the range 0.05-50 Pa s. We find that the spatial form of the swimming gait does not vary across this range of viscosities and that the temporal frequency of the swimming gait only decreases by about 20% with every 10-fold increase in viscosity. Thus, C. elegans swims in low gear,such that its musculature can deliver mechanical force and power nearly 1000-fold higher than it delivers when swimming in water. We find that mutations that disrupt mechanosensation, or the laser killing of specific touch receptor neurons, increase the temporal frequency of the undulating gait, revealing a novel effect of mechanosensory input in regulating the putative central pattern generator that produces locomotion. The adaptability of locomotory gait in C. elegans may be encoded in sensory and motor systems that allow the worm to respond to its own movement in different physical surroundings.
The magnetic compass of domestic chickens,Gallus gallus
Authors: Wiltschko Wolfgang , Freire Rafael , Munro Ursula , Ritz Thorsten , Rogers Lesley , Thalau Peter , Wiltschko Roswitha 
Published: June 2007

SUMMARYBy directional training, young domestic chickens have been shown to use a magnetic compass; the same method has now been used to analyse the functional characteristics and the physical principles underlying the chickens' magnetic compass. Tests in magnetic fields with different intensities revealed a functional window around the intensity of the local geomagnetic field, with this window extending further towards lower than higher intensities. Testing chickens under monochromatic 465 nm blue and 645 nm red light suggested a wavelength dependence, with orientation possible under blue but not under red light. Exposing chickens to an oscillating field of 1.566 MHz led to disorientation, identifying an underlying radical pair mechanism. Local anesthesia of the upper beak, where iron-rich structures have been described as potential magnetoreceptors, did not affect the performance, suggesting that these receptors are not involved in compass orientation. These findings show obvious parallels to the magnetic compass described for European robins,indicating that chickens and small passerines use the same type of magnetic compass mechanism. This suggests that the avian magnetic compass may have evolved in the common ancestor of all present-day birds to facilitate orientation within the home range.
The effect of insect surface features on the adhesion of viscous capture threads spun by orb-weaving spiders
Authors: Opell Brent D. , Schwend Harold S. 
Published: June 2007

SUMMARYSpider orb-webs intercept a broad range of insects and their capture threads must adhere to a range of surface textures. In species of the Araneoidea clade, these capture threads are composed of viscid droplets whose size and spacing differ among species. To determine how droplet profile and insect surface texture interact, we measured the stickiness of viscous threads produced by four species using four insect surfaces that ranged from a smooth beetle elytra to the dorsal surface of a fly abdomen that was covered by large, widely spaced setae. The adhesion of threads to these surfaces differed by as much as 3.5-fold within a spider species and 2.1-fold for the same insect surface between spider species. However, 96% of these differences in stickiness was explained by four variables: the ratio of natural log of droplet volume to setal length, the natural log of droplet volume per mm of thread length, setal surface area, and the area of cuticle not excluded from thread contact by setae. Compared with previous measurements of primitive cribellar capture threads produced by orb weavers of the Deinopoidea clade,viscous threads performed more uniformly over the range of insect surfaces. They also held bug hemelytra, which were densely covered with fine setae, more securely, but held beetle elytra, fly wings and fly abdomens less securely than did viscous threads. Hemelytra may be held more securely because their setae more easily penetrate the viscous boundary layer to establish a greater area of interaction and, after having done so, offer more resistance as they are pulled through this layer. Finely textured surfaces may also have higher effective surface energies and therefore may interact more completely with viscous material.
Energetic cost of producing cyclic muscle force, rather than work, to swing the human leg
Authors: Doke Jiro , Kuo Arthur D. 
Published: June 2007

SUMMARYWe compared two hypothesized energetic costs for swinging the human leg quickly. The first cost is to perform mechanical work on the leg, and the second is to produce muscle force cyclically at high frequencies. Substantial metabolic energy is expended to perform isolated leg swinging, especially at rates greater than the leg's natural pendular frequency. To determine whether the production of muscle force contributes to this cost, we measured oxygen consumption in human subjects (N=6) performing isolated swinging of the leg at frequencies 0.7-1.1 Hz. Amplitude of swing was varied as a function of frequency so that the rate of positive mechanical work performed on the leg remained fixed. We expected that the cost of producing force would increase,in contrast to the cost of performing work. The results showed that average rate of positive mechanical work performed on the leg remained nearly constant as a function of frequency, at 0.073±0.014 W kg-1. Net metabolic rate, however, increased by 53%, from 0.66 W kg-1 to 1.01 W kg-1. Work may be performed on the leg and with a proportional metabolic cost, but it cannot explain the substantial increases observed here. Metabolic energy expenditure appears to increase in proportion with muscle force or torque, and in inverse proportion to duration of force. This energetic cost may be associated with cyclical calcium transport, where rate-limiting of crossbridge attachments may require greater sarcoplasmic calcium concentration at high frequencies of leg swinging to produce the same amplitude of muscle force. It may also be relevant to moving the legs back and forth relative to the body during walking.
Two-phase fuel distribution measurements in a gasoline direct injection engine with an air-assisted injector using advanced optical diagnostics
Authors: Leach B , Zhao H , Li Y , Ma T 
Published: June 2007

The fuel distribution and mixture formation processes within an internal combustion (IC) engine can greatly affect its ignition, combustion, pollutant formation, and fuel consumption. For more than two decades the laser-induced fluorescence (LIF) technique has been successfully applied to the study of mixture formation and distribution. However, in a gasoline direct injection (GDI) engine the fuel may exist in either the liquid or vapour phase, and it is therefore advantageous to be able to observe both phases simultaneously. One means of achieving this is the laser-induced exciplex fluorescence (LIEF) technique. This paper details its application to a single-cylinder GDI engine equipped with an air-assisted fuel injection system and full optical access. Naphthalene and N, N-dimethlylaniline (DMA) tracers were used to track the fuel and were excited using the expanded beam from an XeCl excimer laser at 308 nm. An image doubler and optical filtering system was used to collect the images that were recorded from the front of the cylinder. Early and late injection timings were investigated, and the spray characteristics and mixture formation from the two strategies were found to differ considerably. In order to investigate the effect of absorption on the fluorescence signal, the beam was introduced into the cylinder from three directions. Crank angle resolved results, from the start of injection to the end of the compression stroke, are presented, with an image for each illumination direction and injection timing.