bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electron/infrared-phonon coupling in ABC trilayer graphene
Authors: Zan Xiaozhou , Guo Xiangdong , Deng Aolin , Huang Zhiheng , Liu Le , Wu Fanfan , Yuan Yalong , Zhao Jiaojiao , Peng Yalin , Li Lu , Zhang Yangkun , Li Xiuzhen , Zhu Jundong , Dong Jingwei , Shi Dongxia , Yang Wei , Yang Xiaoxia , Shi Zhiwen , Du Luojun , Dai Qing , Zhang Guangyu 
Published: February 2024

AbstractStacking order plays a crucial role in determining the crystal symmetry and has significant impacts on electronic, optical, magnetic, and topological properties. Electron-phonon coupling, which is central to a wide range of intriguing quantum phenomena, is expected to be intricately connected with stacking order. Understanding the stacking order-dependent electron-phonon coupling is essential for understanding peculiar physical phenomena associated with electron-phonon coupling, such as superconductivity and charge density waves. In this study, we investigate the effect of stacking order on electron-infrared phonon coupling in graphene trilayers. By using gate-tunable Raman spectroscopy and excitation frequency-dependent near-field infrared nanoscopy, we show that rhombohedral ABC-stacked trilayer graphene has a significant electron-infrared phonon coupling strength. Our findings provide novel insights into the superconductivity and other fundamental physical properties of rhombohedral ABC-stacked trilayer graphene, and can enable nondestructive and high-throughput imaging of trilayer graphene stacking order using Raman scattering.
A c-di-GMP signaling module controls responses to iron in Pseudomonas aeruginosa
Authors: Zhan Xueliang , Zhang Kuo , Wang Chenchen , Fan Qiao , Tang Xiujia , Zhang Xi , Wang Ke , Fu Yang , Liang Haihua 
Published: February 2024

AbstractCyclic dimeric guanosine monophosphate (c-di-GMP) serves as a bacterial second messenger that modulates various processes including biofilm formation, motility, and host-microbe symbiosis. Numerous studies have conducted comprehensive analysis of c-di-GMP. However, the mechanisms by which certain environmental signals such as iron control intracellular c-di-GMP levels are unclear. Here, we show that iron regulates c-di-GMP levels in Pseudomonas aeruginosa by modulating the interaction between an iron-sensing protein, IsmP, and a diguanylate cyclase, ImcA. Binding of iron to the CHASE4 domain of IsmP inhibits the IsmP-ImcA interaction, which leads to increased c-di-GMP synthesis by ImcA, thus promoting biofilm formation and reducing bacterial motility. Structural characterization of the apo-CHASE4 domain and its binding to iron allows us to pinpoint residues defining its specificity. In addition, the cryo-electron microscopy structure of ImcA in complex with a c-di-GMP analog (GMPCPP) suggests a unique conformation in which the compound binds to the catalytic pockets and to the membrane-proximal side located at the cytoplasm. Thus, our results indicate that a CHASE4 domain directly senses iron and modulates the crosstalk between c-di-GMP metabolic enzymes.
Deregulated protein homeostasis constrains fetal hematopoietic stem cell pool expansion in Fanconi anemia
Authors: Kovuru Narasaiah , Mochizuki-Kashio Makiko , Menna Theresa , Jeffrey Greer , Hong Yuning , me Yoon Young , Zhang Zhe , Kurre Peter 
Published: February 2024

AbstractDemand-adjusted and cell type specific rates of protein synthesis represent an important safeguard for fate and function of long-term hematopoietic stem cells. Here, we identify increased protein synthesis rates in the fetal hematopoietic stem cell pool at the onset of hematopoietic failure in Fanconi Anemia, a prototypical DNA repair disorder that manifests with bone marrow failure. Mechanistically, the accumulation of misfolded proteins in Fancd2−/− fetal liver hematopoietic stem cells converges on endoplasmic reticulum stress, which in turn constrains midgestational expansion. Restoration of protein folding by the chemical chaperone tauroursodeoxycholic acid, a hydrophilic bile salt, prevents accumulation of unfolded proteins and rescues Fancd2−/− fetal liver long-term hematopoietic stem cell numbers. We find that proteostasis deregulation itself is driven by excess sterile inflammatory activity in hematopoietic and stromal cells within the fetal liver, and dampened Type I interferon signaling similarly restores fetal Fancd2−/− long-term hematopoietic stem cells to wild type-equivalent numbers. Our study reveals the origin and pathophysiological trigger that gives rise to Fanconi anemia hematopoietic stem cell pool deficits. More broadly, we show that fetal protein homeostasis serves as a physiological rheostat for hematopoietic stem cell fate and function.
Carbapenem-resistant Klebsiella pneumoniae capsular types, antibiotic resistance and virulence factors in China: a longitudinal, multi-centre study
Authors: Hu Fupin , Pan Yuqing , Li Heng , Han Renru , Liu Xiao , Ma Ruijing , Wu Yongqin , Lun Heyuan , Qin Xiaohua , Li Jiayin , Wang Aixi , Zhou Min , Liu Bing , Zhou Zhemin , He Ping 
Published: February 2024

AbstractEpidemiological knowledge of circulating carbapenem-resistant Klebsiella pneumoniae (CRKP) is needed to develop effective strategies against this public health threat. Here we present a longitudinal analysis of 1,017 CRKP isolates recovered from patients from 40 hospitals across China between 2016 and 2020. Virulence gene and capsule typing revealed expansion of CRKP capsule type KL64 (59.5%) alongside decreases in KL47 prevalence. Hypervirulent CRKP increased in prevalence from 28.2% in 2016 to 45.7% in 2020. Phylogenetic and spatiotemporal analysis revealed Beijing and Shanghai as transmission hubs accounting for differential geographical prevalence of KL47 and KL64 strains across China. Moderate frequency capsule or O-antigen loss was also detected among isolates. Non-capsular CRKP were more susceptible to phagocytosis, attenuated during mouse infections, but showed increased serum resistance and biofilm formation. These findings give insight into CRKP serotype prevalence and dynamics, revealing the importance of monitoring serotype shifts for the future development of immunological strategies against CRKP infections.
A large dataset of annotated incident reports on medication errors
Authors: Wong Zoie S. Y. , Waters Neil , Liu Jiaxing , Ushiro Shin 
Published: February 2024

AbstractIncident reports of medication errors are valuable learning resources for improving patient safety. However, pertinent information is often contained within unstructured free text, which prevents automated analysis and limits the usefulness of these data. Natural language processing can structure this free text automatically and retrieve relevant past incidents and learning materials, but to be able to do so requires a large, fully annotated and validated corpus of incident reports. We present a corpus of 58,658 machine-annotated incident reports of medication errors that can be used to advance the development of information extraction models and subsequent incident learning. We report the best F1-scores for the annotated dataset: 0.97 and 0.76 for named entity recognition and intention/factuality analysis, respectively, for the cross-validation exercise. Our dataset contains 478,175 named entities and differentiates between incident types by recognising discrepancies between what was intended and what actually occurred. We explain our annotation workflow and technical validation and provide access to the validation datasets and machine annotator for labelling future incident reports of medication errors.
Discrete element simulation of vibration compaction of slag subgrade
Authors: Peng Hu , Jiazhen Chen , Lejin Zhang , Kun Wang , Shuping Wang , Lianyang Chi 
Published: February 2024

AbstractIn this study, to improve the compaction quality and parameters of slag, discrete element models of irregular rock particles (10–60 mm) and circular soil particles (5 mm) were established based on on-site slag screening results. The motion of the vibratory roller was captured by coupling the roadbed model with the roller model in a simulation in which the roller vibrated and compacted the slag subgrade. The results indicated that (1) the best compaction was achieved when the small particle content was 40%, the medium particle content was 20%, and the large particle content was 40%. (2) When the slag was dominated by small rock particles, the optimum compaction frequency was 28 Hz, and when large rock particles dominated, the optimum compaction frequency was 33 Hz. (3) Rock particles were the primary particles that experienced stress in the vibration compaction, and the compressive force and displacement depended on the particle size. (4) The longitudinal and vertical displacements and rotation angles of the soil and rock particles were examined. The results of this study are conducive for advancing the understanding of slag compaction and improving the working efficiency and compaction quality of rollers.
The topology of interpersonal neural network in weak social ties
Authors: Kurihara Yuto , Takahashi Toru , Osu Rieko 
Published: February 2024

AbstractThe strategies for social interaction between strangers differ from those between acquaintances, whereas the differences in neural basis of social interaction have not been fully elucidated. In this study, we examined the geometrical properties of interpersonal neural networks in pairs of strangers and acquaintances during antiphase joint tapping. Dual electroencephalogram (EEG) of 29 channels per participant was measured from 14 strangers and 13 acquaintance pairs.Intra-brain synchronizations were calculated using the weighted phase lag index (wPLI) for intra-brain electrode combinations, and inter-brain synchronizations were calculated using the phase locking value (PLV) for inter-brain electrode combinations in the theta, alpha, and beta frequency bands. For each participant pair, electrode combinations with larger wPLI/PLV than their surrogates were defined as the edges of the neural networks. We calculated global efficiency, local efficiency, and modularity derived from graph theory for the combined intra- and inter-brain networks of each pair. In the theta band networks, stranger pairs showed larger local efficiency than acquaintance pairs, indicating that the two brains of stranger pairs were more densely connected. Hence, weak social ties require extensive social interactions and result in high efficiency of information transfer between neighbors in neural network.
Predictors of microvascular complications in patients with type 2 diabetes mellitus at regional referral hospitals in the central zone, Tanzania: a cross-sectional study
Authors: Shillah Wilfred B. , Yahaya James J. , Morgan Emmanuel D. , Bintabara Deogratius 
Published: February 2024

AbstractMicrovascular complications encompass a group of diseases which result from long-standing chronic effect of diabetes mellitus (DM). We aimed to determine the prevalence of microvascular complications and associated risk factors among patients with type 2 diabetes mellitus (T2DM). A cross-sectional analytical hospital-based study was conducted at Singida and Dodoma regional referral hospitals in Tanzania from December 2021 to September 2022. A total of 422 patients with T2DM were included in the analysis by determining the prevalence of microvascular complications and their predictors using multivariable logistic regression analysis. A two-tailed p value less than 0.05 was considered statistically significant. The prevalence of microvascular complications was 57.6% (n = 243) and diabetic retinopathy was the most common microvascular complication which accounted for 21.1% (n = 89). Having irregular physical activity (AOR = 7.27, 95% CI = 2.98–17.71, p < 0.001), never having physical activity (AOR = 2.38, 95% CI = 1.4–4.01, p = 0.013), being hypertensive (AOR = 5.0, 95% CI = 2.14–11.68, p = 0.030), having T2DM for more than 5 years (AOR = 2.74, 95% CI = 1.42–5.26, p = 0.025), being obese (AOR = 2.63, 95% CI = 1.22–5.68, p = 0.010), and taking anti-diabetic drugs irregularly (AOR = 1.94, 95% CI = 0.15–0.77, p < 0.001) were the predictors of microvascular complications. This study has revealed a significant proportion of microvascular complications in a cohort of patients with T2DM. Lack of regular physical activity, being obese, taking anti-diabetic drugs irregularly, presence of hypertension, and long-standing duration of the disease, were significantly associated with microvascular complications.
Equatorward shift of the boreal summer intertropical convergence zone in Maritime Continent and the impacts on surface black carbon concentration and public health
Authors: Huang Tao , Gu Yefu , Lallemant David , Lau Gabriel N. C. , Sung Joseph J. Y. , Yim Steve H. L. 
Published: February 2024

AbstractIn Maritime Continent, the shift of intertropical convergence zone (ITCZ) location directly regulates the distribution of black carbon and hence affects public health in the region, but the mechanism and human health impacts have not yet been comprehensively revealed. Here we used multiple reanalysis datasets to investigate the long-term shift of seasonal-mean zonal-mean ITCZ location in this region from 1980 to 2014, and to assess the influences on black carbon distribution and the resultant health impact in terms of premature mortality. Results show that recent human-related equatorial warming contributed to an equatorward shift (∼2.1°) of ITCZ location in Maritime Continent. Spatially, the equatorward shift of ITCZ reduced surface black carbon concentration over the maritime area by enhancing updrafts and wet deposition, but raised the concentration in the continental area by inhibiting updrafts. Meanwhile, anomalous low-level northeasterlies weakened summer circulation and prevented black carbon from being transported to the Philippines. Our results also suggest that the equatorward shift decreased ∼13% of black carbon-associated monthly premature mortality in maritime countries, but increased ∼6% of that in continental countries based on the population and mortality rate in 2010. We therefore recommend considering climate change impacts in the design of adaptation strategies against regional air pollution.
Charge transfer mechanism for realization of double negative differential transconductance
Authors: Han Kyu Hyun , Kim Seung-Hwan , Kim Seung-Geun , Kim Jong-Hyun , Song Sungjoo , Yu Hyun-Yong 
Published: February 2024

AbstractWith development of information age, multi-valued logic (MVL) technology utilizing negative differential transconductance (NDT) phenomenon has drawn attention as next-generation computing technology that can replace binary logic. However, because conventional NDT devices primarily use ternary logic, multiple-peak NDT device is required for higher-radix MVL that can process more datasets. Here, van der Waals double-peak anti-ambipolar transistor (AAT) as NDT device was developed by utilizing peak voltage (Vpeak) modulation of NDT peak. For realization of quaternary logic, Vpeak modulation technology was developed through charge transfer mechanism from channel, thereby shifting NDT peak and increasing peak-to-valley current ratio (PVCR). Furthermore, Double-peak AAT was implemented through parallel configuration of two AATs with different Vpeak values. Finally, quaternary inverter with four widely stable logic states was implemented by utilizing the developed double-peak AAT with two distinct NDT peaks and high PVCR. This double-peak AAT is expected to contribute to the development of next-generation MVL technology capable of processing datasets.
Intestinal microbiome and maternal mental health: preventing parental stress and enhancing resilience in mothers
Authors: Matsunaga Michiko , Takeuchi Mariko , Watanabe Satoshi , Takeda Aya K. , Kikusui Takefumi , Mogi Kazutaka , Nagasawa Miho , Hagihara Keisuke , Myowa Masako 
Published: February 2024

AbstractThe number of mothers suffering from mental illness is increasing steadily, particularly under conditions of the coronavirus pandemic. The identification of factors that contribute to resilience in mothers is urgently needed to decrease the risks of poor physical and psychological health. We focused on the risk of parenting stress and psychological resilience in healthy mothers with no psychiatric and physical disorders and conducted two studies to examine the relationships between intestinal microbiota, physical condition, and psychological state. Our results showed that alpha diversity and beta diversity of the microbiome are related to high parenting stress risk. Psychological resilience and physical conditions were associated with relative abundances of the genera Blautia, Clostridium, and Eggerthella. This study helps further understand the gut–brain axis mechanisms and supports proposals for enhancing resilience in mothers.
Reinforcement learning-based control for waste biorefining processes under uncertainty
Authors: Gao Ji , Wahlen Abigael , Ju Caleb , Chen Yongsheng , Lan Guanghui , Tong Zhaohui 
Published: February 2024

AbstractWaste biorefining processes face significant challenges related to the variability of feedstocks. The supply and composition of multiple feedstocks in these processes can be uncertain, making it difficult to achieve economically feasible and sustainable waste valorization for large-scale production. Here, we introduce a reinforcement learning-based framework that aims to control these uncertainties and improve the efficiency of the process. The framework is tested on an anaerobic digestion process and is found to perform better than traditional control strategies. In the short term, it achieves faster target tracking with increased precision and accuracy, while in the long term, it shows adaptive and robust behavior even under additional seasonal supply variability, meeting downstream demand with high probability. This reinforcement learning-based framework offers a promising and scalable solution to address uncertainty issues in real-world biorefining processes. If implemented, this framework could contribute to sustainable waste management practices globally, making waste biorefining processes more economically viable and environmentally friendly.
Small extracellular vesicles in plasma carry luminal cytokines that remain undetectable by antibody-based assays in cancer patients and healthy donors
Authors: Hong Chang Sook , Diergaarde Brenda , Whiteside Theresa L. 
Published: February 2024

Abstract
Background
Small (30–150 nm) extracellular vesicles (sEV), also known as exosomes, play a key role in cell-to-cell signaling. They are produced by all cells, circulate freely and are present in all body fluids. Evidence indicates that cytokines are present on the surface and/or in the lumen of sEV. The contribution of intravesicular cytokines to cytokine levels in plasma are unknown.

Methods
sEV were isolated by ultrafiltration/size exclusion chromatography from pre-cleared plasma obtained from patients with head and neck squamous cell carcinoma (HNSCC) and healthy donors (HDs). Multiplex immunoassays were used to measure cytokine levels in paired untreated and detergent-treated (0.5% Triton X-100) plasma and plasma-derived detergent-treated sEV. Non-parametric tests were used to assess differences in cytokine levels.

Results
The presence of cytokines in sEV isolated from patients’ and HDs’ plasma was confirmed by immunoblots and on-bead flow cytometry. sEV-associated cytokines were functional in various in vitro assays. Levels of cytokines in sEV varied among the HNSCC patients and were generally significantly higher than the levels observed in sEV from HDs. Compared to untreated plasma, levels for the majority (40/51) of the evaluated proteins were significantly higher in detergent-treated plasma (P < 0.0001–0.03). In addition, levels of 24/51 proteins in sEV, including IL6, TNFRII, IL-17a, IFNa and IFNg, were significantly positively correlated with the difference between levels detected in detergent-treated plasma and untreated plasma.

Discussion
The data indicate that sEV-associated cytokines account for the differences in cytokine levels measured in detergent-treated versus untreated plasma. Ab-based assays using untreated plasma detect only soluble cytokines and miss cytokines carried in the lumen of sEV. Permeabilization of sEV with a mild detergent allows for Ab-based detection of sEV-associated and soluble cytokines in plasma. The failure to detect cytokines carried in the sEV lumen leads to inaccurate estimates of cytokine levels in body fluids.
mTert induction in p21-positive cells counteracts capillary rarefaction and pulmonary emphysema
Authors: Lipskaia Larissa , Breau Marielle , Cayrou Christelle , Churikov Dmitri , Braud Laura , Jacquet Juliette , Born Emmanuelle , Fouillade Charles , Curras-Alonso Sandra , Bauwens Serge , Jourquin Frederic , Fiore Frederic , Castellano Rémy , Josselin Emmanuelle , Sánchez-Ferrer Carlota , Giovinazzo Giovanna , Lachaud Christophe , Gilson Eric , Flores Ignacio , Londono-Vallejo Arturo , Adnot Serge , Géli Vincent 
Published: February 2024

AbstractLung diseases develop when telomeres shorten beyond a critical point. We constructed a mouse model in which the catalytic subunit of telomerase (mTert), or its catalytically inactive form (mTertCI), is expressed from the p21Cdkn1a locus. Expression of either TERT or TERTCI reduces global p21 levels in the lungs of aged mice, highlighting TERT non-canonical function. However, only TERT reduces accumulation of very short telomeres, oxidative damage, endothelial cell (ECs) senescence and senile emphysema in aged mice. Single-cell analysis of the lung reveals that p21 (and hence TERT) is expressed mainly in the capillary ECs. We report that a fraction of capillary ECs marked by CD34 and endowed with proliferative capacity declines drastically with age, and this is counteracted by TERT but not TERTCI. Consistently, only TERT counteracts decline of capillary density. Natural aging effects are confirmed using the experimental model of emphysema induced by VEGFR2 inhibition and chronic hypoxia. We conclude that catalytically active TERT prevents exhaustion of the putative CD34 + EC progenitors with age, thus protecting against capillary vessel loss and pulmonary emphysema.
The influence of electromagnetic field models on the damper winding loss and heat calculation results of tubular hydro‐generator
Authors: Xu Meng‐qi , Hu Wen‐hao , Zhou Zhi‐ting , Fan Zhen‐nan 
Published: February 2024

AbstractThis study investigates the effect of electromagnetic field models on tubular hydro‐generator damper winding losses and heat calculations using an on‐site 36‐MW such generator as an example. Sinusoidal steady‐state and transient‐motion electromagnetic field models were established to analyze damper winding losses and heat. Results indicate that, despite exhibiting a substantially higher computational efficiency than the transient‐motion electromagnetic field time‐stepping FE model, the sinusoidal steady‐state electromagnetic field FE model does not comprehensively and reasonably consider several key factors, such as tooth and slot effects, the main magnetic field, and the armature reaction. Consequently, the magnetic field, damper bar loss, and heat derived from this model exhibit considerable deviations from the on‐site generator observations. In comparison, the transient‐motion electromagnetic field time‐stepping FE model, owing to its ability to consider these influencing factors, provides more comprehensive and reasonable results.