bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fine needle aspiration of osteitis fibrosa cystica
Authors: Watson Carolyn W. , Unger Pamela , Kaneko Mamoru , Gabrilove Jacques L. 
Published: February 2007

AbstractThe cytology findings of a fine needle aspiration biopsy from osteitis fibrosa cystica (brown tumor) of the rib in a patient with primary hyperparathyroidism due to parathyroid carcinoma are discussed. Many multinucleated osteoclast‐type giant cells, characteristic of either osteitis fibrosa cystica or benign giant cell tumor of bone, were noted. There were also spindly or fibrillary cells with single, ovoid nuclei, probably of stromal origin.
Appraisal and cytomorphologic analysis of common carcinomas of the breast
Authors: Kline Tilde S. , Kannan Vaidehi , Kline Irwin K. 
Published: February 2007

AbstractThis work is based on 15 years experience with more than 9000 needle aspiration biopsies from the breast performed by a number of clinicians without syringe guns. From 1981 through 1983, 329 carcinomas were detected with a sensitivity of 90%. A positive or suspicious report was issued in 17 of the 32 minimal carcinomas. There were no false‐positive diagnoses. A retrospective comparative study was made on the aspiration biopsy cytology specimens from 65 histologically verified carcinomas: 30 infiltrating ductal, 16 infiltrating lobular, 10 medullary, and 10 colloid carcinomas. Parameters included the pattern, major malignant criteria, and cell measurements by calibrated ocular micrometry. The classic features of each carcinoma and the differential cytomorphology are described.
Herpetic tracheobronchitis detected at bronchoscopy: Cytologic diagnosis by the immunoperoxidase method
Authors: Bedrossian Carlos W. M. , De Arce Eloisa A. L. , Bedrossian Ursula K. , Kelly Linda Van Hook 
Published: February 2007

AbstractHerpes simplex virus (HSV) infection of the respiratory tract requires rapid specific diagnosis to avoid late complications and maximize the efficacy of available drug therapy. We report a method of accomplishing this that was tested in 33 cytologic specimens derived from sputum, washings, or brushings from 25 debilitated elderly males suffering from a variety of underlying neoplastic and nonneoplastic chronic diseases. All specimens had shown either single cells (54%), multinucleated groups (8%), or both (38%); they displayed the ground‐glass appearance (86%), discrete nuclear inclusions (4%), or both (10%), as appreciated by the Papanicolaou stain. The specimens were processed by the peroxidase‐antiperoxidase technique utilizing anti‐HSV‐1 antibody and 3,3′‐diaminobenzidine (DAB) as the chromogen. In six cases, aminoethylcarbazol (AEC) was used for comparison. Twenty‐nine of the 33 specimens (88%) stained positively for HSV‐1 as did control sections of herpetic encephalitis and esophagitis; there were no false positives with appropriate negative controls. All 12 bronchoscopic specimens revealed virocytes with HSV immunopositivity; in contrast, 17 of 21 (80%) sputum specimens were positive for HSV. The strongest positivity was noted in bronchial brushings and washings whereas the only four negative smears were from sputum specimens. The DAB immunostain was coarser and stronger at the periphery of the cytoplasm, and the hematoxylin counterstain permitted a clear identification of nuclear viral changes. With AEC, the immunostain was more vivid and evenly distributed, but counterstaining was impaired due to the greater solubility of the chromogen. The technique is sensitive, reproducible, and less expensive and time‐consuming than electron microscopy (EM) or viral cultures. Diagn Cytopathol 1985;1:292–9.
Differential diagnosis of the pleomorphic aspiration biopsy sample of nonepithelial lesions
Authors: Golouh Rastko , Us‐Krašovec Marija 
Published: February 2007

AbstractThe major problem confronting the cytopathologist in interpretation of the pleomorphic aspiration biopsy sample (ABS) of nonepithelial lesions is to provide a reasonable differential diagnosis, since the presence of cellular pleomorphism alone does not always denote malignancy. One must be fully cognizant of a vast number of pathologic processes to make a correct interpretation. Some indication of the extent of the problem and the potential diagnostic pitfalls are outlined in selection of nonneoplastic processes and benign and malignant tumors of mesenchimal origin presenting as pleomorphic ABSs.
Intraoperative cytology of osseous lesions
Authors: Wilkerson James A. , Crowell Weymouth T. 
Published: February 2007

AbstractCytologic preparations were made from 53 biopsies of osseous and related lesions submitted for intraoperative diagnosis. Smears made by scraping the surface of the lesion and spreading the material obtained on a glass slide were most commonly used. These preparations were found to be valuable adjuncts to frozen sections because cytologic details were so much more clearly visible. Among specific diagnostic features found to be particularly helpful were the large numbers of nuclei found in most of the multinucleated cells from giant‐cell tumors but in few osteoclasts from other lesions; the met achromatic staining of osteoid in air‐dried, Romanowsky‐stained smears from osteosarcomas; and the ease with which multinucleated cells can be identified in smears from chondrosarcomas. The characteristic appearance of chondroblasts from a chondroblastoma; the distinctive appearances of osteoclasts and multinucleated histiocytes from eosinophilic granulomas; the unique appearance of synovial cells from pigmented synovitis; and the ease with which small cells of a metastatic carcinoma could be distinguished from hematopoietic bone‐marrow cells were other significant findings. These and other features of the lesions encountered are presented in greater detail. Diagn Cytopathol 1985;1:5‐12.
Fine‐needle aspiration cytology of lymphadenopathy in homosexual males
Authors: Bottles Kent , Cohen Michael B. , Brodie Howard , Jeffrey R. Brooke , Nyberg David A. , Abrams Donald I. 
Published: February 2007

AbstractWe report five patients who illustrate the usefulness of fine‐needle aspiration cytology (FNAC) in the differential diagnosis of lymphadenopathy in homosexual males. This differential includes hyperplasia, disseminated Kaposi's sarcoma, mycobacterial infections, and non‐Hodgkin's lymphoma. Diagn Cytopathol 1986;2:31‐35.
Ovarian and omental ependymomas in peritoneal washings: Cytologic and immunocytochemical features
Authors: Dekmezian Roupen H. , Sneige Nour , Ordonez Nelson G. 
Published: February 2007

AbstractThe cytologic findings in peritoneal washings of two women, one of whom had an ovarian ependyoma and the other a primary omental ependymoma, are reported. The ependyomas were characterized by the presence of numerous, isolated, spindle and stellate cells as well as groups of cells forming true rosettes. The tumor cells displayed slightly pleomorphic, round‐to‐oval eccentric nuclei and abundant fibrillary cytoplasm with tapering cytoplasmic processes. In addition, one of the patients had numerous papillae and cell clusters with associated psammoma bodies indistinguishable from those found in low‐grade serous carcinoma. The demonstration of glial fibrillary acidic protein (GFAP) in both cases by immunocytochemical procedures indicates the usefulness of this method in cytologic preparations to confirm the diagnosis of these uncommon neoplasms. Diagn Cytopathol 1986;2:62‐68.
Scanning electron microscopy in oral cytology
Authors: Chomette G. , Auriol M. , Vaillant J. M. 
Published: February 2007

AbstractSixty‐three cell smears from oral mucosa were studied by scanning electron microscopy. Among them, smears from ten healthy controls showed three kinds of cells: flat (superficial) cells with linear anastomosing microridges and microvilli; polygonal (intermediate) cells with well‐defined crests between their faces and numerous microvilli; and round (parabasal) cells entirely covered by microvilli. Twenty‐five smears from patients with untreated squamous‐cell carcinoma showed enlarged polymorphous cells (round, globular, and elongated); microvilli, variable in their dimensions, were irregularly distributed on their surfaces. Eighteen smears from patients with severe epithelial dysplasia showed polymorphous cells with discontinuous but obvious edges separating their faces and with irregular microvilli and ridges. Nine smears were also performed in patients with various other mucosal lesions (lichen planus, leukoplakia, white sponge naevus, pemphigus vulgaris, and herpes). All of these smears were studied comparatively between examination of smears and biopsies by light microscopy. The smears were truly reliable, particularly for distinguishing between dysplastic and tumoral cells. Diagn Cytopathol 1986;2:110‐117.
Cytologic detection of cervical granuloma inguinale
Authors: Leiman Gladwyn , Markowitz S. , Margolius Karin A. 
Published: February 2007

AbstractTwo cases of granuloma inguinale detected cytologically on routine Papanicolaou‐stained cervical smears are presented; the cytologic and histologic features are described in detail, and the electron microscopic appearances are shown. The suitability of the Papanicolaou stain in the detection of granuloma inguinale is discussed, with emphasis on possible differential diagnoses to be considered. It is concluded that a definitive diagnosis of granuloma inguinale can be made on routine Papanicolaou‐stained smears showing the following spectrum of changes: intact capillaries indicative of epithelial and stromal ulceration; a marked inflammatory cell infiltrate consisting predominantly of neutrophils; epithelioid histiocytes representing granuloma formation; and Donovan bodies located in characteristic single or multiple intracytoplasmic vacuoles within large histiocytes. Recognition of this cytologic picture may enable early diagnosis, even in nonendemic areas, of a potentially disfiguring venereal disease. Diagn Cytopathol 1986;2:138‐143.
Air drying as a preparatory factor in cytology: Investigation of its influence on dye uptake and dye binding
Authors: Schulte Erik 
Published: February 2007

AbstractThe effect of air‐drying on cytological material is investigated in this article. Smears of rat liver were fixed completely wet and, after air drying, postfixed in ethanol, methanol/formaldehyde/acetic acid (MFA), and formaldehyde. Staining was performed with the thionin‐Feulgen procedure, a standard Roma‐nowsky‐Giemsa stain with azure B‐eosin Y and a Papanicolaou staining variant. The image analysis system IBAS 2000 was applied to evaluate objective criteria of the changes caused by air drying the chromatin texture. Nuclear absorption was measured with a Vickers M 85a Microdensitometer. Air‐drying had striking effects on size and shape of cell nuclei (spreading), on the structure of the nuclear chromatin (chromatin condensation), and on the chromaticity coordinates (hue, saturation, and intensity of nuclear staining). The variations of the chromatin texture and dye‐substrate affinity are attributed to alterations of the tertiary structure of the nuclear proteins. Diagn Cytopathol 1986;2:160–167.
Tracheal aspirate cytology and bronchopulmonary dysplasia
Authors: Rothberg Alan D. , Miot Anne , Leiman Gladwyn 
Published: February 2007

AbstractSequential tracheal aspirates from 39 neonates with hyaline membrane disease were examined to correlate cytological findings with the development of bronchopulmonary dysplasia (BPD). A total of 224 tracheal aspirates were examined from these infants, 15 of whom developed BPD as diagnosed by conventional clinical and radiological criteria. Hyperplastic and metaplastic epithelial changes were observed in all infants studied. Dysplastic changes occurred in 14 of 15 who developed BPD, and 14 of 24 who had a normal outcome (P < 0.02). The exfoliation of dysplastic metaplastic bronchial cells was thus 95% specific and 71% sensitive for the subsequent development of BPD. Factors associated with the development of BPD were very low birth weight and gestational age, persistence of a patent ductus arteriosus, high peak inspiratory pressure (cm of H2O/kg), prolonged assisted ventilation, and rapidity of development of class III (dysplastic) changes in tracheal aspirates. Diagn Cytopathol 1986;2:212‐216.
Aspiration biopsy cytology of metastatic endometrial stromal sarcoma and extragenital mixed mesodermal tumor
Authors: Nguyen Gia‐Khanh , Berendt Richard C. 
Published: February 2007

AbstractAspiration biopsy from metastatic tumors in two cases of endometrial stromal sarcoma and one case of endometrial adenosarcoma revealed malignant endometrial stromal cells with ill‐defined cytoplasm and round or oval hyperchromatic nuclei showing irregular chromatin clumping and conspicuous nucleoli. They were seen mainly in clusters. Aspirate from a metastatic tumor of a mixed mesodermal tumor arising from the omentum showed similar malignant endometrial stroma cells, irregular tight clusters of malignant glandular cells having scanty but well‐defined cytoplasm and vesicular nuclei with conspicuous nucleoli, and fragments of atypical smooth muscle tissue. The diagnostic malignant endometrial stromal cells in those reported cases did not display any distinctive cellular features permitting their cytologic identification. They were difficult to differentiate from those of other types of sarcoma. In a clinical setting, with a known primary endometrial stromal sarcoma or mixed mesodermal tumor, however, a cytodiagnosis of its metastases may be suggested when malignant endometrial stromal‐cell‐like cells are seen in aspirated material, aviating an open‐tissue biopsy. Diagn Cytopathol 1986;2:256–260.
Signet‐ring lymphoma: Report of a case
Authors: Manivel‐Rodriguez J. Carlos , Monroy Marte N. , Ruiz Patricia Alonso‐De , Larraza Oscar , Orazco‐Estevez Hector , Espinoza Alfonso Valenzuela 
Published: February 2007

AbstractSignet‐ring lymphoma is a rare entity in which the synthesis or secretion of immunoglobulins is impaired, producing a signet‐ring appearance to the lymphoma cells. A case of this type of lymphoma is described, and the cytologic aspects of the cells obtained from the fixative fluid are described. Diagn Cytopathol 1986;2:338–340.
The significance of cutaneous metastasis from visceral tumors diagnosed by fine‐needle aspiration biopsy
Authors: Pak Hyun Y. , Foster Barbara A. , Yokota Susan B. 
Published: February 2007

AbstractCutaneous metastases from various visceral organs were studied in 43 patients. The morphologic diagnosis in each case was established by fine‐needle aspiration cytodiagnosis. There were 28 males and 15 females, with median ages of 62 and 61 yr, respectively. The most common primary tumor in men was carcinoma of the lung (35%), followed by malignant melanoma (21%) and carcinoma of the oropharynx (14%). In women, the most frequent primary cancers were carcinoma of the colon (59%) and lung (20%). Metastatic cutaneous lesions were more frequent in the back (23%), upper extremities (21%), and scalp (12%). Median survival from onset of cutaneous metastasis was shortest in primary lung cancer at 3 mo followed by colon at 5 mo and oropharynx at 5.5 mo. Our study confirms that cutaneous metastasis represents a terminal manifestation of the disease due to either hematogenous or lymphatic spread. This study also reiterates the clinical usefulness of needle aspiration biopsy as an alternative diagnostic tool in establishing the presence of cutaneous metastasis. Diagn Cytopathol 1987;3:24–29.
The value of endometrial cytology by scraping in 1, 798 cases: Screening in asymptomatic women and diagnosis in symptomatic ones
Authors: Porrazzi Luigi Coscia , Quarto Ferdinando , Maiello Francesco M. , De Falco Maria L. , Antonucci Tiziana 
Published: February 2007

AbstractA study of 1,798 endometrial cytological samples, obtained with an “endocyte” (a disposable scraping device) and controlled in histology is presented. The method was tested for its usefulness in screening of precancerous lesions and preclinical cancer in 1,248 asymptomatic women and in the diagnosis of endometrial pathology in 550 symptomatic ones. The classification of hyperplasia into benign simplex and complex hyper‐plasia and atypical hyperplasia was followed; atypical hyperplasia, considered real intraepithelial neoplasia, was included in the positive cases. Histological verification was made chiefly on cell block in asymptomatic cytologically negative cases and either on curettage or hysterectomies in the others. The main endometrial cytological patterns are described. On the basis of findings, considering also endocyte's low cost, effectiveness, and ease of use, we think the method could be usefully employed in screening of endometrial cancer for an asymptomatic population. In symptomatic women, the use of histologic procedures is still preferable, and cytology cannot be considered a “first option” method. Diagn Cytopathol 1987;3:112–120.
Fine‐needle aspiration of breast lesions: Role and accuracy in a review of 7, 495 cases
Authors: Fessia Luciano , Botta Giovanni , Arisio Riccardo , Verga Marinella , Aimone Virgilio 
Published: February 2007

AbstractIn the past 10 years, 7,495 cytological breast fine‐needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative results was 93.2% and for positive results was 98.6%, and the accuracy was 94.6%. Inadequate (13.3%) and doubtful samples (8.1%) were excluded from calculation. False‐negative results (82 cases) mainly resulted from sampling errors. False suspicious results (six cases) lessened with increasing experience in breast pathology and with the application of strict diagnostic criteria, but most likely they will never reach zero. Frozen‐section diagnosis could be bypassed only in selected cases. Guidelines on the role of FNA in management of solid breast lesions are given. FNA deserves further evaluation in diagnosing early stages of breast carcinoma: sensitivity was 7.5% in 57 carcinomas in situ, 67.5% in 55 minimally invasive carcinoma, and 92.7% in 538 nonminimally invasive carcinomas. Diagn Cytopathol 1987;3:121–125.
Diagnosis of thymoma by fine‐needle aspiration cytology: Light and electron microscopic study of a case
Authors: Millar Jeremy , Allen Rodney , Wakefield John St John , Buchanan Andrew J. , Gupta Raj K. 
Published: February 2007

AbstractA case of thymoma was diagnosed by fine‐needle aspiration cytology based on an intimate admixture of a biphasic cell population consisting of epithelial cells and lymphocytes from an intrathoracic mass. The diagnosis was later confirmed by light and electron microscopic examination of the tissue. Diagn Cytopathol 1987;3:166–169.
Differential staining techniques in amniotic fluid cytology diagnosis of neural tube defects: A prospective study of 129 pregnancies
Authors: Cohen Margaret L. , Ducatman Barbara S. , Stock Richard J. 
Published: February 2007

AbstractIn order to assess the utility of amniotic fluid cytology in the diagnosis of neural tube defects (NTDs), we performed a prospective study of 129 pregnancies selected for maternal age or history of prior congenital defects or multiple stillbirths. Amniotic fluid obtained between the 16th and 22nd weeks of gestation was cytocentrifuged and stained with hematoxylineosin (H&E), Papanicolaou, and Diff‐Quik. Chromosomal analysis, amniotic fluid alpha fetoprotein (AFP) level, and eventual pregnancy outcome were recorded for each case. The AFP levels were normal in 128 cases, including two closed NTDs (meningomyelocele and encephalocele). Three patients had abnormal chromosomal studies: two with 47, XXX and one with trisomy 21. Of these 128 cases, 16 had inadequate cytologic preparations; the remainder, including the five aforementioned patients, showed predominantly squamous cells, amnion cells, and minicells. Other cell types, including genito‐urinary cells, umbilical cord cells, respiratory cells, and macro‐phages, were also present in smaller numbers. One case exhibited a large population of small, darkly staining neural cells and large macrophages. The AFP in this case was markedly elevated, and the fetus was noted to be anencephalic. We found the H&E to be the preferred stain for both cytologic detail and minicell preservation; however papanicolaou was better for bloody specimens. Amniotic fluid cytology may provide a rapid and inexpensive method of confirming the diagnosis of open NTDs. Diagn Cytopathol 1987;3:271‐7.
Curschmann's spirals in sputum: Histochemical evidence of bronchial gland ductal origin
Authors: Antonakopoulos G. N. , Lambrinaki E. , Kyrkou K. A. 
Published: February 2007

AbstractCurschmann's spirals from 30 patients suffering from chronic bronchitis were examined histochemically by periodic acid‐Schiff (PAS), Alcian blue (AB), PAS‐AB, high‐iron diamine (HID), and HID‐AB stains. Their central core and fibrillar component were found to be rich in neutral mucins and in sulfated and nonsulfated acid mucins, while their outer mantle was rich in neutral and nonsulfated acid mucins only. Curschmann's spirals consist of mucus of high viscosity. Histochemical and morphological evidence suggests that, in addition to their origin in the lumen of smaller bronchi and bronchioles, they are also formed in the ducts of the seromucous bronchial glands. Diagn Cytopathol 1987;3:291‐4.
Immunocytochemical demonstration of Chlamydia infection in the urogenital tracts
Authors: Kobayashi Tadao K. , Ueda Masami , Araki Hirotaka , Toyoda Kazuaki , Ohmori Kenji , Sawaragi Isamu 
Published: February 2007

AbstractThe peroxidase‐antiperoxidase (PAP) technique was applied in cellular samples for the detection of chlamydial infection. Urethral scrapings were obtained from 316 males with clinically suspected urethritis. Positive PAP staining was detected in 118 (37%) of 316 tested. Cellular samples from the endocervix of 25 (54%) of 46 female contacts of males with positive Chlamydia‐infected cells had positive Chlamydia‐PAP staining. Chlamydia was also found in 12 (25%) of 48 male with gonorrhea. Serum IgG antibodies against Chlamydiatrachomatis were evaluated using an enzyme‐linked immunosorbent assay. A total of 29 out of 73 patients had positive Chlamydia serology; of these, 21 were positive for PAP staining, and eight were negative. The determination of Chlamydia antigen by means of the PAP technique appears to be a satisfactory method for detecting Chlamydia in male and female genital specimens; also, the procedure can be done readily in most laboratory settings. Diagn Cytopathol 1987;3:302‐6.
Carcinoids, atypical carcinoids, and small‐cell carcinomas of the lung: Differential diagnosis of fine‐needle aspiration biopsy specimens
Authors: Szyfelbein Wanda M. , Ross Jeffrey S. 
Published: February 2007

AbstractThe acceptance of fine‐needle aspiration biopsy in the diagnostic work‐up of pulmonary masses has resulted in an increased number of neuroendocrine tumors of the lung first encountered as aspiration cytology specimens. The accurate cytologic identification of these neuroendocrine neoplasms is important in that they have relatively specific clinical and prognostic features in contrast to nonneuroendocrine neoplasms. We report on the cytologic features of 46 primary pulmonary neuroendocrine neoplasms initially encountered on fine‐needle aspiration biopsies. The neoplasms are separated into three distinct cytologic groups, including the typical carcinoid (13 cases), the atypical carcinoid (3 cases), and small‐cell carcinomas (30 cases). The clinical features of all cases—and histologic findings when they were available—are also considered. Finally, a detailed cytologic description of the three groups of neuroendocrine neoplasms is presented with emphasis on differential diagnosis including nonneuroendocrine pulmonary neoplasms.