bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Pyrazoloquinoline Derivatives on the Growth of Leishmania donovani Promastigotes
Authors: Al‐Qahtani Ahmed , Siddiqui Yunus M. , Bekhit Adnan A. , El‐Sayed Ola A. , Aboul‐Enein Hassan Y. , Al‐Ahdal Mohammed N. 
Published: October 2005

AbstractA screening study was conducted to examine the effect of a series of synthesized pyrazoloquinoline derivatives on the growth of Leishmania donovani promastigotes. Sixteen compounds were tested, ten of which showed an inhibitory effect on the growth of promastigotes. Compound 1 demonstrated potent antileishmanial activity, followed by compounds 3 and 7. Some compounds showed less significant activities, while others exhibited little or no activity. Some of these compounds may be potential candidates for future treatment of leishmaniasis.
Angiojet catheter‐based thrombectomy in a neonate with postoperative pulmonary embolism
Authors: Feldman Jeremy P. , Feinstein Jeffrey A. , Lamberti John J. , Perry Stanton B. 
Published: October 2005

AbstractA 2‐day‐old male with pulmonary atresia and an intact ventricular septum underwent central aortopulmonary shunt placement and right ventricular outflow tract reconstruction. Postoperatively, the patient had difficulty weaning from mechanical ventilation and was found to have a large thrombus occluding the entire right lower pulmonary artery. The patient underwent successful percutaneous catheter‐based thrombectomy in the early postoperative period with the Possis AngioJet catheter. The patient had immediate improvement in gas exchange and was able to be weaned from the ventilator shortly thereafter. This represents the first published use of mechanical thrombectomy in a postoperative neonate. © 2005 Wiley‐Liss, Inc.
Gemcitabine as frontline treatment for cutaneous T‐cell lymphoma
Authors: Marchi Enrica , Alinari Lapo , Tani Monica , Stefoni Vittorio , Pimpinelli Nicola , Berti Emilio , Pagano Livio , Bernengo Maria Grazia , Zaja Francesco , Rupoli Serena , Pileri Stefano , Baccarani Michele , Zinzani Pier Luigi 
Published: October 2005

AbstractBACKGROUNDBased on the activity of gemcitabine in heavily pretreated patients with cutaneous T‐cell lymphoma (CTCL), the objective of the current study was to determine the role of gemcitabine in the treatment of patients with advanced, untreated CTCL.METHODSBetween June 2002 and February 2004, 32 untreated patients with mycosis fungoides (MF) (n = 26 patients); peripheral T‐cell lymphoma, unspecified (PTCLU) with exclusive skin involvement (n = 5 patients); and Sezary syndrome (SS) (n = 1 patient) were enrolled in a 7‐institution, Phase II trial and treated with gemcitabine. This drug was given on Days 1, 8, and 15 of a 28‐day schedule at a dose of 1200 mg/m2 intravenously over 30 minutes for a total of 6 cycles.RESULTSOf the 32 patients studied, 7 (22%) achieved a complete response (CR) and 17 (53%) achieved a partial response (PR), whereas the remaining 8 patients showed no benefit from the treatment. Five of the CRs were confirmed histologically. The CR and PR rates were found to be the same for patients with MF and PTCLU, respectively. The median duration of CR was 10 months (range, 4–22 mos). Treatment appeared to be well tolerated; hematologic toxicity was mild and no nausea/emesis or organ toxicity was noted.CONCLUSIONSThe results of the current Phase II study demonstrate the activity of gemcitabine as a single agent in untreated CTCL patients. Further studies using gemcitabine in combination, either contemporary or sequentially, with other drugs in patients with advanced stage, untreated CTCL are needed. Cancer 2005. © 2005 American Cancer Society.
Plasma cytokine and P‐selectin levels in advanced malignancy
Authors: Di Nisio Marcello , Niers Tatjana M. , Reitsma Pieter H. , Buller Harry R. 
Published: October 2005

AbstractBACKGROUNDThe survival benefit described in patients with cancer treated with low molecular weight heparin (LMWH) may result from a LMWH‐mediated effect on the immune system or on the cross‐talk between platelets and tumor cells.METHODSPlasma levels of interleukin (IL)‐10, IL‐6, interferon (IFN)‐γ, and P‐selectin were measured in patients with advanced stage malignancy who were randomized to receive standard cancer care with or without the addition of LMWH.RESULTSPatients with IL‐6 levels above the median had a median survival of 6.5 months versus 8.8 months for those with values below this cutoff (P = 0.02). IL‐10 levels were found to be similarly correlated with survival such that IL‐10 concentrations above the detection limit of the assay were associated with a doubled risk of dying in comparison to undetectable IL‐10 (P = 0.02). No significant association was found between survival and circulating levels of IFN‐γ. For P‐selectin, patients with values below the fourth quartile had a median survival of 8.8 months versus 6.5 months for patients with levels above the fourth quartile (P = 0.02). In multivariate analysis, IL‐10 remained an independent unfavorable prognostic factor (hazard ratio, 2.13; 95% confidence interval, 1.08–4.20). In patients treated with LMWH, the plasma levels of IL‐6, IL‐10, IFN‐γ, and P‐selectin demonstrated similar correlations with survival. However, none of the markers was associated with the beneficial survival effects observed with the administration of LMWH.CONCLUSIONSIL‐10, IL‐6, and P‐selectin levels predicted a poor outcome in patients with advanced stage malignancy. The prolongation in survival observed with LMWH in patients with cancer apparently cannot be explained by a LMWH effect on these circulating markers. Cancer 2005. © 2005 American Cancer Society.
How do eating disorders and alcohol use disorder influence each other?
Authors: Franko Debra L. , Dorer David J. , Keel Pamela K. , Jackson Safia , Manzo Mary Pat , Herzog David B. 
Published: October 2005

AbstractObjectiveAlthough eating disorders and alcohol use disorder (AUD) are known to co‐occur, the influence of one on the other has not been studied.MethodIn a prospective study, women diagnosed with either anorexia nervosa (AN; n = 136) or bulimia nervosa (BN; n = 110) were interviewed and assessed for Research Diagnostic Criteria (RDC) AUD every 6–12 months over 8.6 years.ResultsOver one fourth of the sample (n = 66 [27%]) reported a lifetime history of AUD. Ten percent of the study subjects (n = 24) developed AUD over the course of the study. AUD did not influence recovery from either eating disorder. Poor psychosocial functioning and history of substance use predicted prospective onset of an episode of AUD for both diagnostic groups. Unique predictors for AUD for women with AN were depression, overconcern with body image, and vomiting. Recovery from AUD was predicted by group therapy and hospitalization (women with AN) and individual therapy and exercise (women with BN).ConclusionThe influence of eating disorders on AUD appears to be greater than the reverse. A substantial number of patients who initially present with an eating disorder develop alcohol problems over the course of time, suggesting that the risk is an ongoing one that should be monitored by clinicians. © 2005 by Wiley Periodicals, Inc.
Purging disorder: An ominous variant of bulimia nervosa?
Authors: Keel Pamela K. , Haedt Alissa , Edler Crystal 
Published: October 2005

AbstractObjectiveThe current study evaluates the clinical significance and distinctiveness of purging disorder (PD), an eating disorder characterized by recurrent purging in the absence of objective binge episodes (OBE) among normal‐weight individuals.MethodWomen with PD (n = 37), bulimia nervosa (BN; n = 39), or no eating disorder (n = 35) completed clinical assessments. Women with PD (n = 23) and BN (n = 25) completed 6‐month follow‐up assessments.ResultsCompared with controls, both eating‐disordered groups reported significantly higher eating, Axis I, and Axis II pathology. Compared with BN, PD was associated with significantly lower eating concerns, disinhibition, and hunger. At 6‐month follow‐up, rates of remission did not differ significantly between PD and BN, and crossover between disorders was rare.ConclusionPD appears to be a clinically significant and potentially distinctive eating disorder. © 2005 by Wiley Periodicals, Inc.
Ethnicity and sampling bias in binge eating disorder: Black women who seek treatment have different characteristics than those who do not
Authors: Grilo Carlos M. , Lozano Christine , Masheb Robin M. 
Published: October 2005

AbstractObjectiveThe current study investigated sampling bias as it affects recruited clinic samples of Black and White women with binge eating disorder (BED).MethodsClinical characteristics of a recruited clinic sample (35 Black and 302 White consecutively evaluated women) with BED were compared with a community sample of Black and White women with BED drawn from the New England Women's Health Project. The clinic and community groups met the same definition of BED and were assessed with identical methods.ResultsAmong White women, the clinic and community samples differed on some features (higher body mass index [BMI], and greater eating concerns and shape concerns in the clinic sample) but these differences reflected small to moderate effects sizes. In contrast, among Black women, the clinic sample had substantially higher levels (large effect sizes) of several features of eating disorders (eating concerns, dietary restraint, and shape concern), higher (moderate effect size) BMI, but lower frequency of binge eating (moderate effect size) than the community sample. A comparison of Black and White women within the clinic sample revealed little difference in clinical presentation, except for the significantly higher BMI among Black women.ConclusionA sampling bias appears to exist between both White and Black recruited clinic samples of women with BED, although the bias appears to be substantially greater for Black women. Black women with BED who seek treatment have significantly different characteristics than those who do not. © 2005 by Wiley Periodicals, Inc.
Recent Advances in Electrochemical Enzyme Immunoassays
Authors: Díaz‐González María , González‐García María Begoña , Costa‐García Agustín 
Published: October 2005

AbstractEnzyme immunoassays (EIAs) are currently the predominant analytical technique for the quantitative determination of a broad variety of analytes in clinical, medical, biotechnological, and environmental significance. Although the most common detection methods for EIAs are based on spectroscopic measurements, electrochemical techniques, due to their high sensitivity, selectivity, simplicity and low cost, have emerged as a very attractive alternative to carry out the detection step in this kind of assays. The intention of this review is to cover the progress and development in integrating electrochemical detection methods with EIAs, over the past five years.
Adsorptive Stripping Voltammetric Measurements of Trace Molybdenum at the Bismuth Film Electrode
Authors: Wang Joseph , Thongngamdee Sompong , Lu Donglai 
Published: October 2005

AbstractBismuth‐film electrodes on glassy‐carbon substrates have been successfully applied for adsorptive‐stripping voltammetric measurements of trace molybdenum in the presence of chloranilic acid (CAA). The procedure is based on the preconcentration of the molybdenum‐chloranilic acid complex at a preplated bismuth film electrode held at −0.55 V (vs. Ag/AgCl), followed by a negatively‐sweeping square‐wave voltammetric scan. Factors influencing the adsorptive stripping performance, including different ligands, solution pH, CAA concentration, preconcentration time and potential, have been optimized. The response compares favorably with that observed at mercury film electrodes, and is linear over the 5–50 μg/L Mo concentration range (one min preconcentration). A detection limit of 0.2 μg/L molybdenum is obtained following a 10 min accumulation. High stability is indicated from the reproducible response of a 100 μg/L molybdenum solution (n= 60; RSD=2.6%). Applicability to seawater samples is demonstrated.
Genome scans with gene‐covariate interaction
Authors: Peng Jie , Tang Hsiu‐Khuern , Siegmund David 
Published: October 2005

AbstractGenetic models for gene‐covariate interactions are described. Methods of linkage analysis that utilize special features of these models and the corresponding score statistics are derived. Their power is compared with that of simple genome scans that ignore these special features, and substantial gains in power are observed when the gene‐covariate interaction is strong. Quantitative trait mapping in randomly ascertained sibships and affected sibpair mapping are discussed. For the latter case, a simpler statistic is proposed that has similar performance to the score statistic, but does not require the estimation of nuisance parameters. Since the nuisance parameters are not estimable solely from affected sib‐pair data, this statistic would be much easier to apply in practice. Similarities with linkage analysis of models for longitudinal data and multivariate phenotypes are also briefly discussed. Approximations for the P‐value and power are derived under the framework of local alternatives. Genet. Epidemiol. 2005. © 2005 Wiley‐Liss, Inc.
Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: I. µGISAXS and µdiffraction of P450scc
Authors: Nicolini Claudio , Pechkova Eugenia 
Published: October 2005

AbstractUltrasmall P450scc cytochrome microcrystals are grown by classical hanging vapor diffusion and by its modification using homologous protein thin‐film template displaying a long‐range order. The nucleation and growth mechanisms of P450scc microcrystals are studied at the thin cytochrome film surface by a new microbeam grazing incidence small angle X‐ray scattering (µGISAXS) technique developed at the microfocus beamline of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. P450scc cytochrome crystals of about 5 µm are also investigated by synchrotron radiation diffraction in order to attempt a preliminary analysis of the atomic structure of this unique protein system yet unsolved. J. Cell. Biochem. 97: 544–552, 2006. © 2005 Wiley‐Liss, Inc.
The RING finger protein RNF8 recruits UBC13 for lysine 63‐based self polyubiquitylation
Authors: Plans Vanessa , Scheper Johanna , Soler Marta , Loukili Noureddine , Okano Yukio , Thomson Timothy M. 
Published: October 2005

AbstractThe heterodimeric ubiquitin conjugating enzyme (E2) UBC13‐UEV mediates polyubiquitylation through lysine 63 of ubiquitin (K63), rather than lysine 48 (K48). This modification does not target proteins for proteasome‐dependent degradation. Searching for potential regulators of this variant polyubiquitylation we have identified four proteins, namely RNF8, KIA00675, KF1, and ZNRF2, that interact with UBC13 through their RING finger domains. These domains can recruit, in addition to UBC13, other E2s that mediate canonical (K48) polyubiquitylation. None of these RING finger proteins were known previously to recruit UBC13. For one of these proteins, RNF8, we show its activity as a ubiquitin ligase that elongates chains through either K48 or K63 of ubiquitin, and its nuclear co‐localization with UBC13. Thus, our screening reveals new potential regulators of non‐canonical polyubiquitylation. © 2005 Wiley‐Liss, Inc.
Cellular localization of human Rad51C and regulation of ubiquitin‐mediated proteolysis of Rad51
Authors: Bennett Brian T. , Knight Kendall L. 
Published: October 2005

AbstractRad51‐catalyzed homologous recombination is an important pathway for repair of DNA double strand breaks and maintenance of genome integrity in vertebrate cells. Five proteins referred to as Rad51 paralogs promote Rad51 activity and are proposed to act at various, and in some cases, multiple stages in the recombination pathway. Imaging studies of native Rad51 have revealed its cellular response to DNA damage, yet visualization of the paralog proteins has met with limited success. In this study, we are able to detect endogenous Rad51C and Xrcc3 in human cells. In an effort to determine how Rad51, Rad51C, and Xrcc3 influence the pattern of localization of each other over the time course of DNA damage and repair, we have made the unexpected observation that Rad51 degradation via the ubiquitin‐mediated proteasome pathway occurs as a natural part of recombinational DNA repair. Additionally, we find that Rad51C plays an important role in regulating this process. This article contains supplementary material, which may be viewed at the Journal of Cellular Biochemistry website at http://www.interscience.wiley.com/jpages/0730‐2312/suppmat/index.html. © 2005 Wiley‐Liss, Inc.
Regulating leukotriene synthesis: The role of nuclear 5‐lipoxygenase
Authors: Brock Thomas G. 
Published: October 2005

AbstractLeukotrienes are lipid messengers involved in autocrine and paracrine cellular signaling. They are synthesized from arachidonic acid by the 5‐lipoxygenase pathway. Current models of this enzymatic pathway recognize that a key step in initiating leukotriene synthesis is the calcium‐mediated movement of enzymes, including 5‐lipoxygenase, to intracellular membranes. However, 5‐lipoxygenase can be imported into or exported from the nucleus before calcium activation. As a result, its subcellular localization will affect its ability to be activated by calcium, as well as the membrane to which it binds and its interaction with other enzymes. This commentary focuses on the role of 5‐lipoxygenase compartmentation in determining its regulation and, ultimately, leukotriene synthesis. J. Cell. Biochem. © 2005 Wiley‐Liss, Inc.
Introduction of an initiator element in the mouse thymidylate synthase promoter alters S phase regulation but has no effect on promoter bidirectionality
Authors: Kapadia Fehmida , Johnson Lee F. 
Published: October 2005

AbstractThe promoter of the mouse thymidylate synthase (TS) gene lacks a TATAA box and an initiator element, is bidirectional and initiates transcription at multiple start sites across broad initiation windows upstream and downstream of the 30 nt essential promoter region. The TS promoter also plays an essential role in the post‐transcription regulation of TS gene expression during the G1–S phase transition. The goal of this study was to determine if the addition of a TATAA box or an initiator element would have a significant effect on start‐site pattern, promoter bidirectionality and S phase regulation of the TS gene. A TATAA box and/or an initiator element were inserted downstream of the TS essential promoter region, and the modified promoters were used to drive expression of indicator genes. The engineered genes were transfected into cultured mammalian cells, and the effects of the mutations were determined. Addition of the TATAA box and especially the initiator element had a significant effect on the transcription start site pattern, indicating that the elements were functional. Unexpectedly, addition of one or both of these elements had no effect on promoter bidirectionality. However, inclusion of the initiator element led to a significant reduction in S phase regulation of TS mRNA levels, indicating that changes in promoter architecture can perturb normal S phase regulation of TS gene expression. J. Cell. Biochem. © 2005 Wiley‐Liss, Inc.
Cell differentiation dependent expressed CCR6 mediates ERK‐1/2, SAPK/JNK, and Akt signaling resulting in proliferation and migration of colorectal cancer cells
Authors: Brand Stephan , Olszak Torsten , Beigel Florian , Diebold Joachim , Otte Jan‐Michel , Eichhorst Soeren T. , Göke Burkhard , Dambacher Julia 
Published: October 2005

AbstractThe expression of CCL20 (MIP‐3α), which chemoattracts leukocytes to sites of inflammation, has been shown in intestinal epithelial cells (IEC). Aim of this study was to analyze the role of the CCL20 receptor CCR6 in IEC and colorectal cancer (CRC) cells. Expression of CCR6 and CCL20 was analyzed by RT‐PCR and immunohistochemistry. Signaling was investigated by Western blotting, proliferation by MTS assays and chemotactic cell migration by wounding assays. The effect of CCL20 on Fas‐induced apoptosis was determined by flow cytometry. CCR6 and its ligand CCL20 are expressed in IEC. Moreover, CRC and CRC metastases express CCR6, which is upregulated during IEC differentiation. Stimulation of IEC with CCL20 and proinflammatory stimuli (TNF‐α, IL‐1β, LPS) significantly upregulates CCL20 mRNA expression. CCL20 expression was significantly increased in inflamed colonic lesions in Crohn's disease and correlated significantly with the IL‐8 mRNA expression in these lesions (r = 0.71) but was downregulated in CRC metastases. CCL20 activated Akt, ERK‐1/2, and SAPK/JNK MAP kinases and increased IL‐8 protein expression. The CCL20 mediated activation of these pathways resulted in a 2.6‐fold increase of cell migration (P = 0.001) and in a significant increase of cell proliferation (P < 0.05) but did not influence Fas‐induced apoptosis. In conclusion, IEC and CRC express CCL20 and its receptor CCR6. CCL20 expression is increased in intestinal inflammation, while CCR6 is upregulated during cell differentiation. CCR6 mediated signals result in increased IEC migration and proliferation suggesting an important role in intestinal homeostasis and intestinal inflammation by mediating chemotaxis of IEC but also in mediating migration of CRC cells. J. Cell. Biochem. 97: 709–723, 2006. © 2005 Wiley‐Liss, Inc.
Comparison of gated and nongated fast multislice black‐blood carotid imaging using rapid extended coverage and inflow/outflow saturation techniques
Authors: Mani Venkatesh , Itskovich Vitalii V. , Aguiar Silvia H. , Mizsei Gabor , Aguinaldo Juan Gilberto S. , Samber PE Daniel D. , Macaluso Frank M. , Fayad Zahi A. 
Published: October 2005

AbstractPurposeTo comparatively analyze two fast in vivo multislice black‐blood carotid artery vessel wall imaging techniques with and without cardiac gating.Materials and MethodsEight subjects with carotid artery atherosclerosis, and four healthy subjects were studied using two black‐blood multislice techniques: rapid extended coverage double inversion recovery (REX‐DIR), and inflow/outflow saturation band (IOSB) rapid acquisition with relaxation enhancement (RARE) multislice acquisitions. Quantitative, qualitative, and morphometric analyses were performed on images.ResultsGating produced significantly lower values for the REX‐DIR sequence with respect to signal intensity in muscle and the carotid artery wall, whereas it had no effect on flow suppression compared to nongated images. For the IOSB sequences, gating had no significant effect on signal intensity of muscle and the carotid artery wall, but worsened flow suppression. REX‐DIR and IOSB sequences were statistically different with respect to signal intensity of muscle (with REX‐DIR sequences having lower values), while no statistical significance was observed for flow suppression and wall delineation. A morphologic analysis of the vessel wall and lumen comparing REX‐DIR gated, IOSB gated, REX‐DIR nongated, and IOSB nongated sequences revealed no significant differences between the acquisition techniques tested.ConclusionNongated sequences may be used instead of gated sequences in atherosclerotic vessel wall imaging without compromising image quality. This may shorten examination time and improve patient comfort. J. Magn. Reson. Imaging 2005. © 2005 Wiley‐Liss, Inc.