bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unveiling APOL1 haplotypes in a predominantly African-American cohort of kidney transplant patients: a novel classification using probe-independent quantitative real-time PCR
Authors: Dogan Murat , Watkins Christine , Ingram Holly , Moore Nicholas , Rucker Grace M. , Gower Elizabeth G. , Eason James D. , Bhalla Anshul , Talwar Manish , Nezakatgoo Nosratollah , Eymard Corey , Helmick Ryan , Vanatta Jason , Bajwa Amandeep , Kuscu Canan , Kuscu Cem 
Published: April 2024

IntroductionApolipoprotein-L1 (APOL1) is a primate-specific protein component of high-density lipoprotein (HDL). Two variants of APOL1 (G1 and G2), provide resistance to parasitic infections in African Americans but are also implicated in kidney-related diseases and transplant outcomes in recipients. This study aims to identify these risk variants using a novel probe-independent quantitative real-time PCR method in a high African American recipient cohort. Additionally, it aims to develop a new stratification approach based on a haplotype-centric model.MethodsGenomic DNA was extracted from recipient PBMCs using SDS lysis buffer and proteinase K. A quantitative PCR assay with modified forward primers and a common reverse primer enabled us to quantitatively identify single nucleotide polymorphisms (SNPs) and the 6-bp deletion. Additionally, we used Sanger sequencing to verify our QPCR findings.ResultsOur novel probe-independent qPCR effectively distinguished homozygous wild-type, heterozygous SNPs/deletions, and homozygous SNPs/deletions, with at least 4-fold differences. A high prevalence of APOL1 variants was observed (18% two-risk alleles, 34% one-risk allele) in our recipient cohort. Intriguingly, no significant impact of recipient APOL1 variants on transplant outcomes was observed up to 12-month of follow-ups. Ongoing research will encompass more time points and a larger patient cohort, allowing for a comprehensive evaluation of G1/G2 variant subgroups categorized by new haplotype scores, enriching our understanding.ConclusionOur cost-effective and rapid qPCR technique facilitates APOL1 genotyping within hours. Prospective and retrospective studies will enable comparisons with long-term allograft rejection, potentially predicting early/late-stage transplant outcomes based on haplotype evaluation in this diverse group of kidney transplant recipients.
Effect of manual osteopathic techniques on the autonomic nervous system, respiratory system function and head-cervical-shoulder complex—a systematic review
Authors: Stępnik Jakub , Czaprowski Dariusz , Kędra Agnieszka 
Published: April 2024

BackgroundOsteopathic manual techniques are now widely used in medicine worldwide. At present, there are no clear conclusions regarding the possibility of affecting the function of the autonomic nervous system (ANS), respiratory system and head-cervical-shoulder complex by manual osteopathic techniques.ObjectivesThe aim of the study was to review the current literature regarding the possible impact of osteopathic manual techniques on the state of the autonomic nervous system, spirometric parameters of the respiratory system and the state of the head-collar-shoulder complex.MethodsPublications have been searched in the following databases: PubMed, Virtual Health Library and Cochrane Central Register of Controlled Trials. The search strategy included keywords related to manual osteopathic treatment, autonomic nervous system, spirometry, respiratory function and head, neck and shoulder pain. The methodological quality of the included studies was assessed. The PRISMA guidelines were used for the systematic review. Studies from 2010 to 2023 were selected.ResultsUsing the proposed descriptions and manual searches from the literature of other works, 40 studies were found, out of which 22 were rejected because they did not meet the inclusion criteria. The analysis included: 15 randomized controlled trials, 3 pilot studies.ConclusionStudies clearly show the effect of OMT on both spirometric parameters and the condition of the head-collar-shoulder complex. Most often this translates into improved ANS performance, but there are exceptions.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, CRD42023476963.
Transcriptomic analysis identifies the shared diagnostic biomarkers and immune relationship between Atherosclerosis and abdominal aortic aneurysm based on fatty acid metabolism gene set
Authors: Gu Xuefeng , Yu Zhongxian , Qian Tianwei , Jin Yiqi , Xu Guoxiong , Li Jiang , Gu Jianfeng , Li Ming , Tao Ke 
Published: April 2024

Background:Epidemiological research has demonstrated that there is a connection between lipid metabolism disorder and an increased risk of developing arteriosclerosis (AS) and abdominal aortic aneurysm (AAA). However, the precise relationship between lipid metabolism, AS, and AAA is still not fully understood. The objective of this study was to examine the pathways and potential fatty acid metabolism-related genes (FRGs) that are shared between AS and AAA.Methods:AS- and AAA-associated datasets were retrieved from the Gene Expression Omnibus (GEO) database, and the limma package was utilized to identify differentially expressed FRGs (DFRGs) common to both AS and AAA patients. Functional enrichment analysis was conducted on the (DFRGs), and a protein-protein interaction (PPI) network was established. The selection of signature genes was performed through the utilization of least absolute shrinkage and selection operator (LASSO) regression and random forest (RF). Subsequently, a nomogram was developed using the results of the screening process, and the crucial genes were validated in two separate external datasets (GSE28829 and GSE17901) as well as clinical samples. In the end, single-sample gene set enrichment analysis (ssGSEA) was utilized to assess the immune cell patterns in both AS and AAA. Additionally, the correlation between key crosstalk genes and immune cell was evaluated.Results:In comparison to control group, both AS and AAA patients exhibited a decrease in fatty acid metabolism score. We found 40 DFRGs overlapping in AS and AAA, with lipid and amino acid metabolism critical in their pathogenesis. PCBD1, ACADL, MGLL, BCKDHB, and IDH3G were identified as signature genes connecting AS and AAA. Their expression levels were confirmed in validation datasets and clinical samples. The analysis of immune infiltration showed that neutrophils, NK CD56dim cells, and Tem cells are important in AS and AAA development. Correlation analysis suggested that these signature genes may be involved in immune cell infiltration.Conclusion:The fatty acid metabolism pathway appears to be linked to the development of both AS and AAA. Furthermore, PCBD1, ACADL, MGLL, BCKDHB, and IDH3G have the potential to serve as diagnostic markers for patients with AS complicated by AAA.
Inhibiting presynaptic calcium channel motility in the auditory cortex suppresses synchronized input processing
Authors: Deane Katrina E. , Klymentiev Ruslan , Heck Jennifer , Mark Melanie D. , Ohl Frank W. , Heine Martin , Happel Max F. K. 
Published: April 2024

IntroductionThe emergent coherent population activity from thousands of stochastic neurons in the brain is believed to constitute a key neuronal mechanism for salient processing of external stimuli and its link to internal states like attention and perception. In the sensory cortex, functional cell assemblies are formed by recurrent excitation and inhibitory influences. The stochastic dynamics of each cell involved is largely orchestrated by presynaptic CAV2.1 voltage-gated calcium channels (VGCCs). Cav2.1 VGCCs initiate the release of neurotransmitters from the presynaptic compartment and are therefore able to add variability into synaptic transmission which can be partly explained by their mobile organization around docked vesicles.MethodsTo investigate the relevance of Cav2.1 channel surface motility for the input processing in the primary auditory cortex (A1) in vivo, we make use of a new optogenetic system which allows for acute, reversable cross-linking Cav2.1 VGCCs via a photo-cross-linkable cryptochrome mutant, CRY2olig. In order to map neuronal activity across all cortical layers of the A1, we performed laminar current-source density (CSD) recordings with varying auditory stimulus sets in transgenic mice with a citrine tag on the N-terminus of the VGCCs.ResultsClustering VGCCs suppresses overall sensory-evoked population activity, particularly when stimuli lead to a highly synchronized distribution of synaptic inputs.DiscussionOur findings reveal the importance of membrane dynamics of presynaptic calcium channels for sensory encoding by dynamically adjusting network activity across a wide range of synaptic input strength.
Neurotype and participatory autism knowledge predict perceptions of an autistic employee in the workplace
Authors: Szechy Kathryn A. , O'Donnell Lisa A. 
Published: April 2024

IntroductionAutistic adults are unemployed at much higher rates than the general population. Differences due to autistic traits, including social skills differences, are often barriers to autistic adults obtaining and retaining employment in non-autistic workplaces. The double empathy problem (DEP) posits that autistic social differences are not due to assumed autistic social impairment but to mutual misunderstandings within the autistic/non-autistic social interaction. Consistent with the DEP, autistic adults are more likely to accurately interpret the behavior of an autistic employee, compared to non-autistic adults. This study examined additional factors, beyond neurotype, that are associated with accurate interpretation of the behavior of an autistic employee.MethodsA sample of 73 autistic and 140 non-autistic participants read a vignette about a hypothetical autistic employee in a non-autistic workplace having a difficult workday. Participants completed questions about their interpretation of the employee's behavior, an autism knowledge measure, autism experience questions, and demographic information.ResultsGeneral autism knowledge, but not autism experience, was an influential predictor of accurate behavior interpretation toward the autistic employee and negated the predictive influence of education level, income, and gender identity of participants (OR = 1.12, p &lt; 0.001). However, regardless of the level of autism knowledge, autistic participants continued to be significantly more likely to accurately interpret the behavior of the autistic employee, compared to non-autistic participants (OR = 2.94, p = 0.02).DiscussionResults continue to contribute support for the DEP within the context of autistic employment challenges. Findings also point to autism education as a promising autism workplace support in addressing the DEP and barriers autistic adults face with obtaining and retaining employment.
Access to a tailored mobile application enhances medication adherence among young users of antidepressants
Authors: Larsen Rønnaug Eline , Hole Kristine , Selle Maria Lie , Johannessen Landmark Cecilie , Krogstad Tonje , Holm Lene Berge 
Published: April 2024

Introduction:Patients’ adherence to antidepressants is generally reported to be poor. This study examined whether users of selective serotonin reuptake inhibitors (SSRIs) and serotonin and norepinephrine reuptake inhibitors (SNRIs) enhance medication adherence following access to a mobile application (app) tailored for this patient group. The study addresses the implementation phase of medication adherence.Methods:The study was a single group pre-post intervention design. Data were collected using the validated OsloMet Adherence-to-medication Survey tool (OMAS-37) before and after app access. Pre-app access survey (Survey 1) was conducted via social media and online newspapers, encompassing 445 SSRI/SNRI users aged 18 years and above. Post-app access survey (Survey 2) was sent to 103 SSRI/SNRI users from Survey 1. Wilcoxon Signed Rank Test compared pre- and post-intervention adherence measurements. Pearson’s chi-square tests and Fisher’s exact tests compared study population categories.Results:Forty-two SSRI/SNRI users, median age 26 (IQR 17), 93% identifying as female, used the app while using the same antidepressant during the 2-month period between gaining access to the app and Survey 2. There was a statistically significant reduction in non-adherence score post-app access (z = 3.57, n = 42, p &lt; 0.001) with medium effect size (r = 0.39), indicating enhanced adherence. Total non-adherence score decreased by 39% from pre-to post-access, and there was a 12% decrease in users scoring equivalent with poor adherence (score &lt;2) post-access. Twenty-nine of 37 non-adherence causes improved, with three showing statistical significance. Of 42 responders, 50% (n = 21) indicated using the app one to two times, while 50% (n = 21) more than three times. Approximately 69% (n = 28) found it useful, and 43% (n = 18) felt safer in their use of antidepressants after access to the app. No significant preference was observed for the app over alternative sources of information.Discussion:Enhanced medication adherence was observed among antidepressant users following access to the tailored app. Further studies are warranted to evaluate the app applicability to a broader range of antidepressants users or other patient groups, encompassing those in the initiation phase of medication adherence. The app is intended as an easily accessible supplement to the information and advice provided by prescribing physicians and dispensing pharmacists.
Physiology of deep closed circuit rebreather mixed gas diving: vascular gas emboli and biological changes during a week-long liveaboard safari
Authors: Balestra Costantino , Lévêque Clément , Mrakic-Sposta Simona , Vezzoli Alessandra , Wauthy Pierre , Germonpré Peter , Tillmans Frauke , Guerrero François , Lafère Pierre 
Published: April 2024

Introduction: Diving decompression theory hypothesizes inflammatory processes as a source of micronuclei which could increase related risks. Therefore, we tested 10 healthy, male divers. They performed 6–8 dives with a maximum of two dives per day at depths ranging from 21 to 122 msw with CCR mixed gas diving.Methods: Post-dive VGE were counted by echocardiography. Saliva and urine samples were taken before and after each dive to evaluate inflammation: ROS production, lipid peroxidation (8-iso-PGF2), DNA damage (8-OH-dG), cytokines (TNF-α, IL-6, and neopterin).Results: VGE exhibits a progressive reduction followed by an increase (p &lt; 0.0001) which parallels inflammation responses. Indeed, ROS, 8-iso-PGF2, IL-6 and neopterin increases from 0.19 ± 0.02 to 1.13 ± 0.09 μmol.min−1 (p &lt; 0.001); 199.8 ± 55.9 to 632.7 ± 73.3 ng.mg−1 creatinine (p &lt; 0.0001); 2.35 ± 0.54 to 19.5 ± 2.96 pg.mL−1 (p &lt; 0.001); and 93.7 ± 11.2 to 299 ± 25.9 μmol·mol−1 creatinine (p = 0.005), respectively. The variation after each dive was held constant around 158.3% ± 6.9% (p = 0.021); 151.4% ± 5.7% (p &lt; 0.0001); 176.3% ± 11.9% (p &lt; 0.0001); and 160.1% ± 5.6% (p &lt; 0.001), respectively.Discussion: When oxy-inflammation reaches a certain level, it exceeds hormetic coping mechanisms allowing second-generation micronuclei substantiated by an increase of VGE after an initial continuous decrease consistent with a depletion of “first generation” pre-existing micronuclei.
Relationship between teachers’ professional identity and career satisfaction among college teachers: role of career calling
Authors: Wu Jinshun , Ghayas Saba , Aziz Aiman , Adil Adnan , Niazi Sadia 
Published: April 2024

ObjectivesProfessional identity has been found an important determinant of career satisfaction of employees working in various fields. Teachers’ professional identity is a relatively less explored domain. Considering the importance of teacher’s career satisfaction, the current study was designed to test the role of career calling in the relationship between teachers’ professional identity and career satisfaction.MethodsA sample of teachers (N = 300) with (n = 115) men and (n = 185) women was recruited from the colleges of Sargodha City, Pakistan with an age range from 24 to 45 (M = 31.3, SD = 6.4). A purposive sampling technique was employed for data collection. Variables of the present study were operationalized through the Professional Identity Scale, Brief Calling Scale, and Career Satisfaction Scale.ResultsPath analysis revealed that normative identity did not have any direct or indirect effects on career satisfaction and career calling. Whereas affective and efficacy identity demonstrated significant positive direct effects on career calling whereas career calling had significant positive direct effect on career satisfaction. Efficacy identity also had a significant positive direct effect on career satisfaction. Finally, both affective and efficacy identity had significant positive indirect effects on career satisfaction via career calling.ConclusionThe findings of the current study may help in devising plans to improve the career satisfaction of teachers working in the higher education sector.
Understanding the patient and supporter journey in cocaine use disorder
Authors: Leclair Denise , Waye Katherine M. , Gomez-Mancilla Baltazar , Kiluk Brian D. , Karanam Ananda Krishna , Banerjee Partha S. , Muthusamy Velusamy Shanmuganathan , Maahs Suzanne 
Published: April 2024

BackgroundThere is a paucity of literature describing experiences and journey of individuals with cocaine use disorder (CUD) and supporters who care for them. The aim of this study was to understand and document the journey of individuals with current CUD, those in CUD remission, and supporters.MethodsThe online bulletin board (OBB) is a qualitative tool where participants engage in an interactive discussion on a virtual forum. After completing a 15-minute screening questionnaire determining eligibility, individuals in CUD remission and supporters participated in an OBB for 60 minutes, split across 8 days over 2 weeks. Individuals with current CUD participated in a one-time virtual focus group discussion for 90 minutes.ResultsIndividuals in CUD remission (n=35) were from Brazil, France, Spain, the UK, and the US; those with current CUD (n=5) and supporters (n=6) were from the US. Key insights were that individuals with current CUD were seeking a ‘euphoric high’ that cocaine provides. Those in CUD remission described a ‘euphoric high’ when they first tried cocaine, but over time it became harder to re-create this feeling. Individuals in CUD remission expressed a ‘rollercoaster’ of emotions from when they first started using cocaine to when they stopped. Supporters were sad, isolated, and worried about a potential cocaine overdose for their loved ones with CUD.ConclusionThe study provides valuable insights into the experiences and journey of individuals with CUD and their supporters. Data generated from this study gives insights into this under-served and growing population.
How does embracing an outdoor lifestyle and sense of responsibility impact plastic reduction efforts?
Authors: Riordan Oliver , Richter Isabell 
Published: April 2024

IntroductionPlastic pollution in coastal regions poses severe environmental challenges. This research aims to examine the impact of an individual's outdoor lifestyle and their perceptions of responsibility for litter management-specifically the roles of governments, businesses, non-governmental organizations (NGOs), Cornwall council, and volunteers-on their engagement in activities aimed at reducing plastic pollution.MethodsThe study surveyed 972 individuals in Cornwall, UK, to gather data on their perceptions and behaviors related to plastic reduction initiatives. The survey employed binomial logistic regression tests to analyse the relationship between respondents' views on responsibility for litter management and their participation in ten different plastic-reducing activities.ResultsThe analysis revealed that individuals' perceptions of who should be responsible for managing litter significantly influence their likelihood of participating in plastic reduction activities. Notably, respondents who viewed national governments, businesses, and volunteers as responsible were more likely to engage in outdoor plastic reduction efforts, such as beach clean-ups. In contrast, attributing responsibility to the Cornwall council and NGOs was negatively correlated with participation in some plastic-reducing activities.DiscussionThe findings suggest that perceptions of responsibility play a crucial role in motivating individuals to participate in plastic reduction efforts. The positive correlation between viewing certain entities (governments, businesses, and volunteers) as responsible and increased participation highlights the potential for targeted interventions. Recommendations for enhancing plastic reduction participation include governmental policy changes, collaboration with outdoor sports groups, and the implementation of community-focused social marketing strategies to foster a collective sense of responsibility and action.
Identifying optimal candidates for primary tumor surgery in patients with metastatic head and neck cancer
Authors: Liang Qi-Wei , Zhuang Shuang-Hao , Li Sheng 
Published: April 2024

BackgroundPrimary tumor surgery (PTS) may enhance survival among part of patients with metastatic head and neck cancer (mHNC). Herein, a predictive model was needed to construct to identify who can gain benefit remarkably from tumor resection.MethodsData of patients with mHNC were extracted from the Surveillance, Epidemiology, and End Results (SEER) database. The best cut-off value of age were analyzed using the X-tile software. One-to-one PSM, Kaplan–Meier method, and log-rank test were performed for survival analysis.The independent factors determined using the multivariate Cox proportional hazard regression were used to construct the nomogram.ResultsA total of 1,614 patients diagnosed with mHNC were included; among them, 356 (22.0%) underwent a surgical procedure for the excision of the primary tumor. cancer-specific survival (CSS) was remarkably prolonged in the PTS group relative to the non-PTS group following PSM [Median:19 months vs. 9 months; hazard ratio (HR) 0.52, P &lt; 0.001]. Patients with mHNC who were younger than 52 years old, had well-differentiated tumors, had T1 and N0 stages, and were married at the time of the study may have significantly benefited from PTS. In addition, we constructed a nomogram based on the factors that independently affect the CSS in multivariate Cox analysis. The nomogram showed excellent discrimination in both the training and validation sets (AUC: 0.732 and 0.738, respectively).ConclusionA practical predictive model was constructed to determine the appropriate patients with mHNC, who would benefit from surgical resection.