bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identical Outcome After Autologous or Allogeneic Genoidentical Hematopoietic Stem-Cell Transplantation in First Remission of Acute Myelocytic Leukemia Carrying Inversion 16 or t(8;21): A Retrospective Study From the European Cooperative Group for Blood and Marrow Transplantation
Authors: Gorin Norbert-Claude , Labopin Myriam , Frassoni Francesco , Milpied Noel , Attal Michel , Blaise Didier , Meloni Giovanna , Iori Anna P. , Michallet Mauricette , Willemze Roel , Deconninck Eric , Harousseau Jean-Luc , Polge Emmanuelle , Rocha Vanderson 
Published: May 2008

Purpose Patients with acute myelocytic leukemia carrying inversion 16 (inv16) or t(8;21) have a better initial response to high-dose cytarabine than patients without these chromosomal abnormalities. They presently do not undergo transplantation in first remission (CR1), but there is concern about late relapses. Patients and Methods From 1990 to 2004, 325 adult patients received transplantations in CR1 (159 patients with inv16 and 166 patients with t(8;21), including 35 and 60 patients, respectively, with additional chromosomal abnormalities). Genoidentical allografts were performed in 64 patients with inv16 and 81 patients with t(8;21), and autografts were performed in 95 patients with inv16 and 85 patients with t(8;21). Results In patients with inv16, after allogeneic and autologous transplantation, the 5-year leukemia-free survival (LFS) rates were 59% and 66% (P = .5), the relapse incidence (RI) rates were 27% and 32% (P = .45), and the transplantation-related mortality (TRM) rates were 14% and 2% (P = .003), respectively. Female patients had a lower RI and a higher LFS. Additional chromosomal abnormalities, compared with no additional abnormalities, were associated with lower RI rate (12% v 34%, respectively; P = .01) and higher 5-year LFS rate (78% v 59%, respectively; P = .04). In patients with t(8;21), after allogeneic and autologous transplantation, the 5-year LFS rates were 60% and 66% (P = .69), the RI rates were 15% and 28% (P = .03), and the TRM rates were 24% and 6% (P = .003), respectively. Younger age and a lower WBC count at diagnosis were associated with a lower TRM and a better LFS. The TRM was lower and the RI was higher in patients with autologous transplantations versus allogeneic transplantations. Conclusion Both autologous and allogeneic transplantation resulted in similar outcomes.
Predictive Biomarkers of Chemotherapy Efficacy in Colorectal Cancer: Results From the UK MRC FOCUS Trial
Authors: Braun Michael S. , Richman Susan D. , Quirke Philip , Daly Catherine , Adlard Julian W. , Elliott Faye , Barrett Jennifer H. , Selby Peter , Meade Angela M. , Stephens Richard J. , Parmar Mahesh K.B. , Seymour Matthew T. 
Published: May 2008

Purpose Candidate predictive biomarkers for irinotecan and oxaliplatin were assessed in 1,628 patients in Fluorouracil, Oxaliplatin, CPT-11: Use and Sequencing (FOCUS), a large randomized trial of fluorouracil alone compared with fluorouracil and irinotecan and compared with fluorouracil and oxaliplatin in advanced colorectal cancer. Methods The candidate biomarkers were: tumor immunohistochemistry for MLH1/MSH2, p53, topoisomerase-1 (Topo1), excision repair cross-complementing gene 1 (ERCC1), O-6-methylguanine-DNA-methyltranserase (MGMT), and cyclooxygenase 2 (COX2); germline DNA polymorphisms in GSTP1, ABCB1, XRCC1, ERCC2, and UGT1A1. These were screened in more than 750 patients for interaction with benefit from irinotecan or oxaliplatin; two markers (Topo1 and MLH1/MSH2) met criteria to be taken forward for analysis in the full population. Primary end points were progression-free survival (PFS) and overall survival. Results One thousand three hundred thirteen patients (81%) were assessable for Topo1 immunohistochemistry (low, < 10%; moderate, 10% to 50%; or high, > 50% tumor nuclei). In patients with low Topo1, PFS was not improved by the addition of either irinotecan (hazard ratio [HR], 0.98; 95% CI, 0.78 to 1.22) or oxaliplatin (HR, 0.85; 95% CI, 0.68 to 1.07); conversely, patients with moderate/high Topo1 benefited from the addition of either drug (HR, 0.48 to 0.70 in all categories; interaction P = .005; overall, P = .001 for irinotecan; P = .05 for oxaliplatin). High Topo1 was associated with a major overall survival benefit with first-line combination chemotherapy (HR, 0.60; median benefit, 5.3 months); patients with moderate or low Topo1 did not benefit (HR, 0.92 and 1.09, respectively; interaction P = .005). MLH1/MSH2 did not show significant interaction with treatment, although the low rate of loss (4.4%) limits the power of the study for this biomarker. Conclusion Topo1 immunohistochemistry identified subpopulations that did or did not benefit from irinotecan, and possibly also from oxaliplatin. If verified independently, this information will contribute to the individualization of treatment for colorectal cancer.
High Expression of Macrophage Colony-Stimulating Factor in Peritumoral Liver Tissue Is Associated With Poor Survival After Curative Resection of Hepatocellular Carcinoma
Authors: Zhu Xiao-Dong , Zhang Ju-Bo , Zhuang Peng-Yuan , Zhu Hong-Guang , Zhang Wei , Xiong Yu-Quan , Wu Wei-Zhong , Wang Lu , Tang Zhao-You , Sun Hui-Chuan 
Published: May 2008

Purpose To investigate prognostic values of the intratumoral and peritumoral expression of macrophage colony-stimulating factors (M-CSF) in hepatocellular carcinoma (HCC) patients after curative resection. Patients and Methods Expression of M-CSF and density of macrophages (MΦ) were assessed by immunohistochemistry in tissue microarrays containing paired tumor and peritumoral liver tissue from 105 patients who had undergone hepatectomy for histologically proven HCC. Prognostic value of these and other clinicopathologic factors was evaluated. Results Neither intratumoral M-CSF nor MΦ density was associated with overall survival (OS) or disease-free survival (DFS). High peritumoral M-CSF and MΦ density, which correlated with large tumor size, presence of intrahepatic metastasis, and high TNM stage, were independent prognostic factors for both OS (P = .001 and P < .001, respectively) and DFS (P = .001 and P = .003, respectively) and affected incidence of early recurrence. In a small HCC subset, peritumoral M-CSF was also correlated with both OS and DFS (P = .038 and P = .001, respectively). The combination of peritumoral M-CSF and MΦ had a better power to predict the patients' death and disease recurrence (P < .001 for both). Conclusion High peritumoral M-CSF and MΦ were associated with HCC progression, disease recurrence, and poor survival after hepatectomy, highlighting the importance of peritumoral tissue in the recurrence and metastasis of HCC. M-CSF and MΦ may be targets of postoperative adjuvant therapy.
Incidence of Venous Thromboembolism in Patients With Ovarian Cancer Undergoing Platinum/Paclitaxel–Containing First-Line Chemotherapy: An Exploratory Analysis by the Arbeitsgemeinschaft Gynaekologische Onkologie Ovarian Cancer Study Group
Authors: Fotopoulou Christina , duBois Andreas , Karavas Alexandros N. , Trappe Ralf , Aminossadati Behnaz , Schmalfeldt Barbara , Pfisterer Jacobus , Sehouli Jalid 
Published: May 2008

Purpose Venous thromboembolism (VTE) has been associated with negative prognosis in cancer patients. Most series reporting on VTE have included different tumor types not differentiating between recurrent or primary disease. Data regarding the actual impact of VTE on primary advanced ovarian cancer (AOC) are limited. Patients and Methods Between 1995 and 2002, the Arbeitsgemeinschaft Gynaekologische Onkologie Ovarian Cancer Study group (AGO–OVAR) recruited 2,743 patients with AOC in three prospectively randomized trials on platinum paclitaxel-based chemotherapy after primary surgery. Pooled data analysis was performed to evaluate incidence, predictors, and prognostic impact of VTE in AOC. Survival curves were calculated for the VTE incidence. Univariate analysis and Cox regression analysis were performed to identify independent predictors of VTE and mortality. Results Seventy-six VTE episodes were identified, which occurred during six to 11 cycles of adjuvant chemotherapy; 50% of them occurred within 2 months postoperatively. Multivariate analysis identified body mass index higher than 30 kg/m2 and increasing age as independent predictors of VTE. International Federation of Gynecology and Obstetrics stage and surgical radicality did not affect incidence. Overall survival was significantly reduced in patients with VTE (median, 29.8 v 36.2 months; P = .03). Multivariate analysis identified pulmonary embolism (PE), but not deep vein thrombosis alone, to be of prognostic significance. In addition, VTE was not identified to significantly affect progression-free survival. Conclusion Patients with AOC have their highest VTE risk within the first 2 months after radical surgery. Only VTE complicated by symptomatic PE have been identified to have a negative impact on survival. Studies evaluating the role of prophylactic anticoagulation during this high risk postoperative period are warranted.
Genetic Analysis of Krüppel-Like Zinc Finger 11 Variants in 5864 Danish Individuals: Potential Effect on Insulin Resistance and Modified Signal Transducer and Activator of Transcription-3 Binding by Promoter Variant −1659G&gt;C
Authors: Gutiérrez-Aguilar Ruth , Froguel Philippe , Hamid Yasmin H. , Benmezroua Yamina , Jørgensen Torben , Borch-Johnsen Knut , Hansen Torben , Pedersen Oluf , Neve Bernadette 
Published: May 2008

Abstract
Context: The transcription factor Krüppel-like zinc finger 11 (KLF11) has been suggested to contribute to genetic risk of type 2 diabetes (T2D). Our previous results showed that four KLF11 variants, in strong linkage disequilibrium (LD block including +185 A&gt;G/Gln62Arg and −1659 G&gt;C) were associated with T2D in a north European case-control study. Here we further analyzed these variants for T2D association in a general Danish population and assess their possible effect on gene function.
Methods: We genotyped Gln62Arg variant, representative for the LD block, in 5864 subjects of the INTER99 study to assess association to T2D and glucose metabolism-related quantitative traits. We studied effects of LD-block variants on KLF11 function and in particular, the effect of −1659G&gt;C on transcriptional regulation of KLF11 using EMSA, chromatin immunoprecipitation, gene reporter assays, and small interfering RNA transfection.
Results: We could not confirm T2D association of the KLF11 LD block, however, in glucose-tolerant subjects; it was significantly associated with higher fasting serum insulin and C-peptide levels and increased homeostasis model assessment insulin resistance indexes (P = 0.00004, P = 0.006, and P = 0.00002, respectively). In addition, binding of signal transducer and activator of transcription (STAT)-3 to the wild-type (−1659G&gt;C) allele stimulated gene transcription, whereas STAT3 did not bind onto the mutant allele.
Conclusions: We showed that KLF11 may interfere with glucose homeostasis in a Danish general population and that STAT3-mediated up-regulation of KLF11 transcription was impaired by the −1659G&gt;C variant. Overall, KLF11 variants may have a deleterious effect on insulin sensitivity, although that may not be sufficient to lead to T2D.
Parental authority, research interests and children's right to decide in medical research – an uneasy tension?
Authors: Swartling Ulrica , Helgesson Gert , Hansson Mats G , Ludvigsson Johnny 
Published: May 2008

There is an increased focus on, and evidence of, children's capability to both understand and make decisions about issues relating to participation in medical research. At the same time there are divergent ideas of when, how and to what extent children should be allowed to decide for themselves. Furthermore, little is known about parents' views on these matters, an important issue since they often provide the formal consent. In this questionnaire study of 2500 families in south-east Sweden (with and without research experience) we explored parents' views on issues relating to information, consent and research data. We found that parents are generally positive about supplying their child with individual information (93.3%; median age 7) and assuring the child's consent/assent to participation (74.3%; median age 12). However, parents' views vary regarding the extent to which children should influence research data: as many as 47–61% of our sample were opposed to children's rights to decide about the use and storage of biological samples and natural history data. Parents who are opposed to child consent and a wider influence on their research participation argue that parental authority and research quality are two important factors opposing enhanced child influence. Drawing on this, we underline the need to discuss how to balance children's rights against parental autonomy and research interests before implementing any standardized protocols granting children the right to consent and revoke data in long-term research.
Effect of Collection Tube Type and Preanalytical Handling on Myeloperoxidase Concentrations
Authors: Shih Jessie , Datwyler Saul A , Hsu Stephen C , Matias Matthew S , Pacenti David P , Lueders Christian , Mueller Christian , Danne Oliver , Möckel Martin 
Published: May 2008

Abstract
Background: Myeloperoxidase (MPO) has shown potential as a marker for cardiovascular disease. Limited studies have been published with a variety of sample types, resulting in a wide range of MPO values. Little is known or understood about the impact of collection tube type and preanalytical handling of specimens for MPO determination.
Method: MPO concentration was determined by use of the ARCHITECT® MPO research use assay, which is currently under development. Samples were collected into multiple anticoagulant collection tubes from donors and patients presenting to the emergency department with symptoms of acute coronary syndromes. Whole blood was stored on ice or at room temperature for predetermined time periods. We also evaluated serum and plasma after centrifugation followed by storage at room temperature, 2–8 °C, and below −10 °C.
Results: Baseline sample concentrations were dependent on collection tube type as well as handling conditions. MPO concentrations were consistently higher in samples collected in serum and heparin plasma tubes than in samples in EDTA or citrate tubes. Spike recovery was acceptable in all sera and plasma tested, indicating that the increased MPO concentrations were not due directly to an anticoagulant interference.
Conclusions: The collection tube type and preanalytical handling are critical for accurate and consistent MPO measurement. The preferred anticoagulant and tubes are the EDTA or EDTA plasma preparation tube. MPO concentrations in samples collected in these tubes are stable before centrifugation as whole blood as well as plasma after processing.
Rapid Determination of Monozygous Twinning with a Microfabricated Capillary Array Electrophoresis Genetic-Analysis Device1
Authors: Yeung Stephanie H I , Medintz Igor L , Greenspoon Susan A , Mathies Richard A 
Published: May 2008

Abstract
Background: Microfabricated genetic-analysis devices have great potential for delivering complex clinical diagnostic technology to the point of care. As a demonstration of the potential of these devices, we used a microfabricated capillary array electrophoresis (μCAE) instrument to rapidly characterize the familial and genotypic relationship of twins who had been assigned fraternal (dizygous) status at birth.
Methods: We extracted the genomic DNA from buccal samples collected from the twin sons, the parents, another sibling, and an unrelated control individual. We then carried out multiplex PCR amplification of sequences at 16 short tandem repeat loci commonly used in forensic identity testing. We simultaneously separated the amplicons from all of the individuals on a μCAE device and fluorescently detected the amplicons with single-base resolution in &lt;30 min.
Results: The genotypic analysis confirmed the identical status of the twins and revealed, in conjunction with the medical data, that their twin status arose from the rarer dichorionic, diamniotic process.
Conclusions: The ability to rapidly analyze complex genetic samples with μCAE devices demonstrates that this approach can help meet the growing need for rapid genetics-based diagnostics.
Composition of callus resin of Norway spruce, Scots pine, European larch and Douglas fir
Authors: Holmbom Thomas , Reunanen Markku , Fardim Pedro 
Published: May 2008

Abstract
On damage of conifer trees, oleoresin is exuded onto the tree stem. The oleoresin is typically composed of monoterpenes and resin acids. It serves to protect the tree from dehydration and microbial attack. Callus resin, in the traditional German term “Überwallungsharz”, is a resin exuded from the callus tissue formed as the wound is closed by annual growth. These resins can usually be found as nodules or lumps on the rim of the closing wound. Both types of resin, collected from Picea abies, Pinus sylvestris, Larix decidua and Pseudotsuga menziesii, were analyzed separately by GC/GC-MS. Expectedly, the oleoresin samples were composed of resin acids. However, the callus resins were of a completely different composition as they are composed primarily of lignans and hydroxycinnamic acid derivatives. In addition, the L. decidua sample contained significant amounts of abietol and abietadiene. The P. abies and L. decidua callus resins were found to be essentially resin acid-free. The components identified in the callus resin samples have previously been identified as minor components in oleoresin and bark samples. The potential for the inadvertent inclusion of callus resin in previously analyzed samples of oleoresin, bark and ingrown (dead) knots is highlighted.
Compressive stress-strain properties of natural materials treated with aqueous NaOH
Authors: Ishikura Yukiko , Nakano Takato 
Published: May 2008

Abstract
Physical and cushioning properties of wood samples treated with various concentrations of NaOH aqueous solutions have been investigated based on compressive stress-strain curves. The shapes of curves changed essentially at NaOH concentrations above 10% as a threshold concentration. The stress-strain curves were analyzed based on the assumption that a rule of mixtures is applicable in the case of a very small range of displacement. The form factor n was also calculated. The values of n of the wood samples treated with NaOH whose concentration was higher than 10% were different from those of the wood samples treated with NaOH whose concentration was less than 10%. The shape changes, longitudinal contraction and twisting of the wood samples are related to NaOH concentration. The energy-absorption efficiency parameter E and ideality parameter I were calculated based on stress-strain curves according to the literature. Also, these parameters change above the threshold concentration of 10%. It was concluded that the cushioning properties of wood treated with alkali concentrations higher than 10% are improved. Due to the shape change of wood samples, the efficiency of energy-absorption properties is higher.
Identification of selected log characteristics from computed tomography images of sugar maple logs using maximum likelihood classifier and textural analysis
Authors: Wei Qiang , Leblon Brigitte , Chui Ying Hei , Zhang Shu Yin 
Published: May 2008

AbstractIn recent years, computed tomography (CT) was investigated to acquire internal log information non-destructively. This paper studied the feasibility of identifying internal log characteristics in CT images by means of maximum likelihood classifier. The log characteristics to be identified include heartwood, sapwood, inner bark, and knots in sugar maple. A total of 100 CT images were sampled from one log to develop the classifier and 20 images were selected from another log for validation. Besides spectral and distance features, textural features were also assessed. In total, nine of them were selected as the input features for the classifier based on the class separability analysis. The classifier developed in this study produced overall accuracies of 79.8% and 72.2% for the training images and the validation images, respectively. This study indicates that the developed maximum likelihood classifier relying on a combination of spectral, textural, and distance features may be applicable to identify the internal log characteristics in the CT images of sugar maple.
The reduction in the fibre saturation point of wood due to chemical modification using anhydride reagents: A reappraisal
Authors: Hill Callum A.S. 
Published: May 2008

Abstract
The data from a previous study of the moisture adsorption of Corsican pine modified with linear chain carboxylic acid anhydrides (acetic, propionic, butyric, valeric, hexanoic) has been reanalysed so that moisture content is reported as a percentage of cell wall mass only. As part of this work, the change in fibre saturation point (FSP, determined from fits of the Hailwood-Horrobin model) of the modified wood has been determined as a function of weight percentage gain (WPG). The change in FSP is not determined by the extent of hydroxyl substitution of the cell wall, but is related to WPG only (as reported previously). This is also true for the relationship between monolayer water, polylayer water, molecular weight per sorption site at saturation and WPG. However, there is no simple linear relationship between calculated FSP and WPG. When the FSP was determined by solute exclusion methods, the reduction thereof could be simply explained as due to bulking of the cell wall by the bonded acyl substituents. This is not the case when the FSP is determined from adsorption isotherms. This result is interpreted as an ambiguity of determination of FSP from projections of sorption isotherms. Differences in FSP determined by the two methods (sorption versus solute exclusion) are considered to be due to incomplete wetting of the lignocellulosic material when absorbing moisture from the initial dry state.
A comprehensive analysis of the relation of cellulose microfibril orientation and lignin content in the S2 layer of different tissue types of spruce wood (Picea abies (L.) Karst.)
Authors: Jungnikl Karin , Koch Gerald , Burgert Ingo 
Published: May 2008

Abstract
A possible relation between cellulose microfibril angle and lignin content in the S2 layer was investigated by X-ray diffraction and cellular UV microspectrophotometry on spruce tissues [Picea abies] with different structural features and chemical composition. A strong correlation was not found, neither for the individual tissue types nor for the compiled data of all tissues. As the data did not confirm the findings in former studies, further examinations are necessary concerning a possible general interrelation between microfibril angle and lignin content.