bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainable and Healthy Gastronomy in Costa Rica – Betting on Sustainable Diets
Authors: Echandi Marcela Dumani , Masis Patricia Sedó , Viquez Randall García , Rodriguez Roberto Azofeifa 
Published: April 2024

Abstract
Current consumption patterns in Costa Rica, and other countries, are often driven by misinformation and lack of knowledge about the nutritional value and sustainability of food products, which leads to unhealthy diets and loss of food biodiversity. In 2012, Costa Rica launched a multi-stakeholder initiative, The National Plan on Healthy and Sustainable Gastronomy (hereafter referred to as the Plan), to reverse this trend. Healthy and Sustainable Gastronomy (HSG) is an innovative paradigm for the sustainability of natural resources based on the consumers’ decision to prepare and enjoy food to be healthy. It is one of various approaches and solutions provided by One Planet Network, which hosts the UN Sustainable Food Systems Programme. Costa Rica’s Plan promotes a new healthy and sustainable national cuisine as a driving force to create a sustainable food system and improve the health of the population through diet. The cuisine is designed to increase the use of local, traditional and plant-based foods and aims to reduce obesity and micronutrient deficiencies. Additional benefits are food security, increased food biodiversity and tourism through gastronomic food culture. This case study explains the concept of HSG and how Costa Rica implemented it to change consumer behaviour; support local producers; achieve national goals for sustainable development; promote tourism and improve population health. Important progress has been made, but lack of information and awareness about linkages connecting food production and consumption is a major barrier. Information challenges include a lack of traceability systems and eco-labelling to build consumer trust, ongoing consumer education, and ensuring production will satisfy demand. Overall, the authors believe that Costa Rica will act as a model of HSG for other countries to follow in order to achieve sustainable food production and consumption, bridging the gap between emerging markets and Western economies.

Information
© CAB International 2019
Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill
Authors: Kharey Gurpreet S , Palace Vince , Whyte Lyle , Greer Charles W 
Published: April 2024

Abstract
The overall impact of a crude oil spill into a pristine freshwater environment in Canada is largely unknown. To evaluate the impact on the native microbial community, a large-scale in situ model experimental spill was conducted to assess the potential role of the natural community to attenuate hydrocarbons. A small volume of conventional heavy crude oil (CHV) was introduced within contained mesocosm enclosures deployed on the shoreline of a freshwater lake. The oil was left to interact with the shoreline for 72 h and then free-floating oil was recovered using common oil spill response methods (i.e. freshwater flushing and capture on oleophilic absorptive media). Residual polycyclic aromatic hydrocarbon (PAH) concentrations returned to near preoiling concentrations within 2 months, while the microbial community composition across the water, soil, and sediment matrices of the enclosed oligotrophic freshwater ecosystems did not shift significantly over this period. Metagenomic analysis revealed key polycyclic aromatic and alkane degradation mechanisms also did not change in their relative abundance over the monitoring period. These trends suggest that for small spills (&lt;2 l of oil per 15 m2 of surface freshwater), physical oil recovery reduces polycyclic aromatic hydrocarbon concentrations to levels tolerated by the native microbial community. Additionally, the native microbial community present in the monitored pristine freshwater ecosystem possesses the appropriate hydrocarbon degradation mechanisms without prior challenge by hydrocarbon substrates. This study corroborated trends found previously (Kharey et al. 2024) toward freshwater hydrocarbon degradation in an environmentally relevant scale and conditions on the tolerance of residual hydrocarbons in situ.
The role of ion homeostasis in adaptation and tolerance to acetic acid stress in yeasts
Authors: Antunes Miguel , Sá-Correia Isabel 
Published: April 2024

Abstract
Maintenance of asymmetric ion concentrations across cellular membranes is crucial for proper yeast cellular function. Disruptions of these ionic gradients can significantly impact membrane electrochemical potential and the balance of other ions, particularly under stressful conditions such as exposure to acetic acid. This weak acid, ubiquitous to both yeast metabolism and industrial processes, is a major inhibitor of yeast cell growth in industrial settings and a key determinant of host colonization by pathogenic yeast. Acetic acid toxicity depends on medium composition, especially on the pH (H+ concentration), but also on other ions’ concentrations. Regulation of ion fluxes is essential for effective yeast response and adaptation to acetic acid stress. However, the intricate interplay among ion balancing systems and stress response mechanisms still presents significant knowledge gaps. This review offers a comprehensive overview of the mechanisms governing ion homeostasis, including H+, K+, Zn2+, Fe2+/3+, and acetate, in the context of acetic acid toxicity, adaptation, and tolerance. While focus is given on Saccharomyces cerevisiae due to its extensive physiological characterization, insights are also provided for biotechnologically and clinically relevant yeast species whenever available.
Human costal cartilage, tooth cavities, and femur nutrient canals—new niches for insects used in forensic entomology
Authors: Tomsia Marcin , Grzywacz Andrzej , Szpila Krzysztof , Walczak Kinga , Mahlerová Karolina , Vaněk Daniel , Matuszewski Szymon 
Published: April 2024

Abstract
The study aimed to analyze the entomological material collected during 13 autopsies performed on the unidentified cadavers revealed at different stages of decay in the Upper Silesia Region (Poland) over 2016–2022. During the preparation of human tissues for genetic identification, we revealed larvae, puparia, and adult insects in previously undescribed locations: costal cartilage, femur nutrient canals (foramen nutrients), and tooth cavities. The taxonomical assessment was done using morphological examination or DNA barcoding, where necessary. Based on our observations, we conclude that the apical constriction, foramen, and cavities may serve as migration paths inside teeth, and the femur nutrient canals to the bone marrow. The study also revealed that Necrobia ruficollis (Fabricius, 1775) and Pyralidae Latreille, 1802 (Phycitinae) moths can form pupal chambers inside the costal cartilage, indicating that these insects can complete their life cycle inside this cache. We believe that the newly reported locations of carrion insects in human remains may be relevant to forensic entomology, as they provide new opportunities to collect insect evidence.
New citizen science initiative enhances flowering onset predictions for fruit trees in Great Britain
Authors: Wyver Chris , Potts Simon G , Pitts Richard , Riley Mike , Janetzko Gerard , Senapathi Deepa 
Published: April 2024

Abstract
Accurately predicting flowering phenology in fruit tree orchards is crucial for timely pest and pathogen treatments and for the introduction of managed pollinators. Making predictions requires large datasets of flowering dates, which are often limited to single locations. Consequently, resulting phenology predictions are not representative across larger geographic areas. Citizen science may offer a solution to this data gap, with millions of biological records across a wide range of taxa recorded annually. Here, a new citizen science platform called “FruitWatch” is introduced, monitoring flowering dates of fruit trees in Great Britain. The objectives of this study are to assess the suitability of FruitWatch submissions to 1) detect latitudinal variation in flowering onset dates, 2) parameterize existing phenology modelling frameworks, and 3) make predictions of flowering onset dates across Great Britain for a single year.
Using data for four cultivars from 2022, linear models reveal significant latitudinal delays in flowering onset of as much as 1.49±0.63 days per degree latitude further north (Pear ‘Conference’), with significant delays also seen in Cherry ‘Stella’ (1.39±0.48 days) and Plum ‘Victoria’ (1.22±0.18 days). FruitWatch informed phenology modelling frameworks performed well for predicting flowering onset, with root-mean-square Error values of predictions from validation datasets ranging between 4.6 (‘Victoria’) and 8.0 (‘Conference’) days. The parameterized models also provided realistic flowering onset predictions across Great Britain in 2022, with earlier flowering dates predicted in warmer areas. These findings demonstrate the potential of citizen science data to offer growers cultivar- and location-specific phenology predictions to help inform orchard management.
Differential analysis of immunoglobulin gene expression pattern in chickens of distinct breeds and developmental periods
Authors: Qiu Yanbo , Yi Xiaohua , Tang Xiaoqin , Wei Yanpei , Zhang Beibei , Duan Shunan , Wang Shuhui , Sun Xiuzhu 
Published: April 2024

Abstract
Immunoglobulin is an essential component of the body’s defense against pathogens, aiding in the recognition and clearance of foreign antigens. Research concerning immunoglobulin gene and its diversity of expression across different breeds within the same species is relatively scarce. In this study, we employed RACE (Rapid Amplification of cDNA Ends) technology, prepared DNA libraries, performed high-throughput sequencing, and conducted related bioinformatics analysis to analyze the differences in immunoglobulin gene diversity and expression at different periods in Hy-line brown hens, Lueyang black-bone chickens, and Beijing-You chickens. The study found that the composition of chicken immunoglobulin genes is relatively simple, with both the light chain and heavy chain having a functional V gene. Additionally, the mechanisms of immunoglobulin diversity generation tended to be consistent among different breeds and periods of chickens, primarily relying on abundant junctional diversity, somatic hypermutation (SHM), and gene conversion (GCV) to compensate for the limitations of low-level V(D)J recombination. As the age increased, the junctional diversity of IgH and IgL tended to diversify and showed similar expression patterns among different breeds. In the three chicken breeds, the predominant types of mutations observed in IGHV and IGLV SHM were A to G and G to A transitions. Specifically, IGLV exhibited a preference for A to G mutations, whereas IGHV displayed a bias toward G to A mutations. The regions at the junctions between framework regions (FR) and complementarity-determining regions (CDR) and within the CDR regions themselves are typically prone to mutations. The locations of GCV events in IGLV and IGHV do not show significant differences, and replacement segments are concentrated in the central regions of FR1, CDR, and FR2. Importantly, gene conversion events are not random occurrences. Additionally, our investigation revealed that CDRH3 in chickens of diverse breeds and periods the potential for diversification through the incorporation of cysteine. This study demonstrates that the diversity of immunoglobulin expression tends to converge among Hy-line brown hens, Lueyang black-bone chickens, and Beijing-You chickens, indicating that the immunoglobulin gene expression mechanisms in different breeds of chickens do not exhibit significant differences due to selective breeding.
Neurobehavioral Symptom Profiles for the Behavioral Assessment Screening Tool in Chronic Traumatic Brain Injury
Authors: Juengst Shannon B. , Wright Brittany , DeMello Annalyn , Vos Leia , Biney Fedora , Novelo Luis Leon , Williams Michael 
Published: April 2024

Objective:
To identify neurobehavioral symptom profiles among persons with chronic traumatic brain injury (TBI) using the Behavioral Assessment Screening Tool (BAST) and to consider participant characteristics that differ between profile groups.

Setting:
Community.

Participants:
Participants (n = 615) were English-speaking adults (≥18) and had a self-reported history of at least one TBI of any severity.

Design:
Secondary analysis of cross-sectional data.

Main Measures:
The BAST measures neurobehavioral symptoms in the domains of Negative Affect, Fatigue, Executive Dysfunction, Impulsivity, and Substance Misuse.

Results:
Using latent profile analysis (LPA), we identified 3 different neurobehavioral profiles. Overall symptom frequency and differences in the pattern of symptom frequency across domains differentiated the profile groups. Average domain scores differed significantly across the profiles (P < .001) for all domains except Fatigue (P = .076). Those in profile 3 (High-Risk group) reported the most frequent symptoms across all domains (similar Negative Affect frequency as profile 1). Substance Misuse was especially high in this group. Compared to profile 2 (High Negative Affect group), participants in profile 1 (Moderate-Risk group) endorsed significantly more frequent (and more variable) symptoms across all BAST domains, particularly Impulsivity and Substance Misuse. Participants in profile 2 endorsed the least frequent symptoms across all domains. Demographic comparison showed that groups differed based on gender, age, and injury severity (mild vs moderate-severe), with profile 3 composed of the most men and the most persons in early adulthood, and profile 2 composed of the most women and those with mild TBI.

Conclusions:
We differentiated 3 neurobehavioral symptom profiles among persons with chronic TBI and determined differences in sociodemographic factors between the groups. Future research should focus on validating these profiles in another sample of individuals with chronic TBI. Characterizing persons according to multidimensional symptom profiles could allow for more tailored approaches to predict and prevent long-term negative outcomes.
The Protective Effects of Acteoside and Total Glycosides of the Leaves of Rehmannia on Three Types of Glomerular Structural Cells Under High Glucose and Inflammatory Stimulation
Authors: Liu Yuyang , Gao Wenya , Wang Mengxiao , Bian Baolin , Zhou Yanyan , Wei Xiaolu , Si Nan , Wang Hongjie , Zhao Haiyu , Sato Takashi 
Published: April 2024

ABSTRACT

Background:
The challenge of chronic glomerulonephritis necessitates innovative strategies for preventing renal function deterioration. Acteoside (ACT), the primary bioactive compound of total glycosides of the leaves of Rehmannia (DHYZG), has been demonstrated availability and safety in reducing proteinuria, showing promising prospects in the treatment of kidney disease. This study aimed to elucidate the impact of ACT and DHYZG on glomerular structural cells and key proteins of rat glomeruli under several injury states.

Methods:
Employing 3-(4,5)-dimethylthiazolyl(-Z-Y1)-3,5-diphenyltetrazolium bromide (MTT) and Western blot methodologies, the investigation assessed varying concentrations of ACT and clinical concentration of DHYZG on cell viability and intracellular biomarkers. Specifically, the study explored the influence of ACT on connective tissue growth factor (CTGF), transforming growth factor-β (TGF-β), matrix metalloproteinase-2 (MMP-2), and MPP in rat mesangial cells, endothelial cells, and podocytes stimulated by lipopolysaccharide (LPS), high glucose, and interleukin-1β (IL-1β). Additionally, the impact of high glucose on angiotensin II (Ang II) expression in endothelial cells was investigated.

Results:
ACT and DHYZG demonstrated a protective effect on all cell types, and ACT administration shows a significant dose-dependent response. These compounds attenuated the expression of CTGF and TGF-β in LPS-stimulated mesangial cells and reduced the expression of MMP-2 and MMP-9 in all three cell types following IL-1β stimulation. Moreover, the high glucose-induced expression of Ang II in endothelial cells was mitigated.

Conclusion:
ACT and DHYZG exhibit a pronounced protective effect on glomerular cells, with ACT being the primary contributor to the therapeutic efficacy of DHYZG. Notably, ACT demonstrates a significant superiority over DHYZG in terms of both proliferation and cytokine expression in glomerular endothelial cells.
Blood Purification for the Treatment of Chronic Kidney Disease-associated Pruritus
Authors: Marcello Matteo , Ramírez-Guerrero Gonzalo , Reis Thiago , Ronco Claudio 
Published: April 2024

ABSTRACT
Chronic kidney diseases-associated pruritus (CKD-aP) is a term that indicates the itching associated to end stage kidney disease without any apparent cause. This symptom leads to decreased quality of life and increased risk of long-term morbidities and mortality. The pathogenesis of uremic pruritus is still unknown, however, different theories have merged: uremic toxins deposition, immune system dysregulation, peripheral neuropathy, and opioid imbalance. While in the last decade, research focused prevalently on pharmaceutical treatment of CKD-aP, extracorporeal therapies have also evolved providing an alternative but efficient treatment option. In this review, we assess the role of different extracorporeal methods on the treatment of CKD-aP.
Suppression of bone resorption by Mori Radicis Cortex through NFATc1 and c-Fos signaling-mediated inhibition of osteoclast differentiation
Authors: Hong Sooyeon , Cho Hye-Rin , Kim Jae-Hyun , Kim Minsun , Lee Sumin , Yang KyuJin , Lee Yujin , Sohn Youngjoo , Jung Hyuk-Sang 
Published: April 2024

Background:
Mori Radicis Cortex (MRC) is the root bark of the mulberry family as Morus alba L. In Korea, it is known as “Sangbaegpi”. Although MRC has demonstrated anti-inflammatory and antioxidant effects, its specific mechanisms of action and impact on osteoporosis remain poorly understood.

Methods:
To investigate the antiosteoporosis effect of MRC, we examined the level of osteoclast differentiation inhibition in receptor activator of nuclear factor kappa-Β ligand (RANKL)-induced-RAW 264.7 cells and animal models of ovariectomy (OVX) with MRC. Serum analysis in OVX animals was investigated by enzyme-linked immunosorbent assay (ELISA), and bone density analysis was confirmed by micro-computed tomography (micro-CT). The expression analysis of nuclear factor of activated T cells 1 (NFATc1) was confirmed by immunohistochemistry (IHC) in femur tissue. In addition, osteoclast differentiation inhibition was measured using tartrate-resistant acid phosphatase (TRAP). mRNA analysis was performed using reverse transcription-polymerase chain reaction (RT-PCR), and the protein expression analysis was investigated by western blot.

Results:
Micro-CT analysis showed that MRC effectively inhibited bone loss in the OVX-induced rat model. MRC also inhibited the expression of alkaline phosphatase (ALP) and TRAP in serum. Histological analysis showed that MRC treatment increased bone density and IHC analysis showed that MRC significantly inhibited the expression of NFATc1. In RANKL-induced-RAW 264.7 cells, MRC significantly reduced TRAP activity and actin ring formation. In addition, MRC significantly inhibited the expression of NFATc1 and c-Fos, and suppressed the mRNA expression.

Conclusion:
Based on micro-CT, serum and histological analysis, MRC effectively inhibited bone loss in an OVX-induced rat model. In addition, MRC treatment suppressed the expression of osteoclast differentiation, fusion, and bone resorption markers through inhibition of NFATc1/c-Fos expression in RANKL-induced RAW 264.7 cells, ultimately resulting in a decrease in osteoclast activity. These results demonstrate that MRC is effective in preventing bone loss through inhibiting osteoclast differentiation and activity.
Exosomal MALAT1 from macrophages treated with high levels of glucose upregulates LC3B expression via miR-204-5p downregulation
Authors: Shyu Kou-Gi , Wang Bao-Wei , Pan Chun-Ming , Fang Wei-Jen , Lin Chiu-Mei 
Published: April 2024

Background:
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) plays a critical role in the pathophysiology of diabetes-related complications. However, whether macrophage-derived MALAT1 affects autophagic activity under hyperglycemic conditions is unclear. Therefore, we investigated the molecular regulatory mechanisms of macrophage-derived MALAT1 and autophagy under hyperglycemic conditions.

Methods:
Hyperglycemia was induced by culturing macrophages in 25 mM glucose for 1 hour. Exosomes were extracted from the culture media. A rat model of carotid artery balloon injury was established to assess the effect of MALAT1 on vascular injury. Reverse transcription, real-time quantitative polymerase chain reaction, western blotting, immunohistochemical staining, and luciferase activity assays were performed.

Results:
Stimulation with high levels of glucose significantly enhanced MALAT1 expression in macrophage-derived exosomes. MALAT1 inhibited miR-204-5p expression in macrophage-derived exosomes under hyperglycemic conditions. siRNA-induced silencing of MALAT1 significantly reversed macrophage-derived exosome-induced miR-204-5p expression. Hyperglycemic treatment caused a significant, exosome-induced increase in the expression of the autophagy marker LC3B in macrophages. Silencing MALAT1 and overexpression of miR-204-5p significantly decreased LC3B expression induced by macrophage-derived exosomes. Overexpression of miR-204-5p significantly reduced LC3B luciferase activity induced by macrophage-derived exosomes. Balloon injury to the carotid artery in rats significantly enhanced MALAT1 and LC3B expression, and significantly reduced miR-204-5p expression in carotid artery tissue. Silencing MALAT1 significantly reversed miR-204-5p expression in carotid artery tissue after balloon injury. MALAT1 silencing or miR-204-5p overexpression significantly reduced LC3B expression after balloon injury.

Conclusion:
This study demonstrated that hyperglycemia upregulates MALAT1. MALAT1 suppresses miR-204-5p expression and counteracts the inhibitory effect of miR-204-5p on LC3B expression in macrophages to promote vascular disease.