bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen 1 gel may improve the regenerative capacity of minced adult and preosteoarthritic cartilage
Authors: Jahn Jannik , Halm‐Pozniak Agnieszka , Klutzny Marcus , Noll Michaela , Stärke Christian , Lohmann Christoph H. , Bertrand Jessica 
Published: February 2024

AbstractPurposeMinced cartilage implantation (MCI) is an evolving technique for the treatment of osteochondral lesions. It was hypothesised that mincing of cartilage may affect chondrocyte viability and phenotype and that embedding in collagen 1 gel results in an improved outcome. The objective of this study was to evaluate the impact of cartilage mincing and whether collagen 1 gel mediates beneficial effects on the chondrocyte phenotype and viability.MethodsHuman cartilage samples from 11 patients undergoing total knee arthroplasty were collected and minced according to the MCI protocol. Minced cartilage was cultured for 1 week with and without embedding in collagen 1 gel and was compared with unminced cartilage flakes as control. Quantitative reverse transcription‐PCR and immunohistochemical staining for the chondrocyte marker genes SOX9, COL2, ACAN, COL10 and MMP13 were used to examine the chondrocyte phenotype. Cell death was assessed by the terminal deoxynucleotidyl transferase dUTP nick‐end labeling assay.ResultsIncreased chondrocyte cell death of cultured cartilage after mincing was observed. Chondrocytes from minced cartilage exhibited significantly decreased expression and protein levels of homeostatic and hypertrophic chondrocyte markers. Embedding in collagen 1 gel showed no positive effect on viability. However, remarkable is the increased expression of ACAN and the preserved protein level of SOX9 in the collagen 1‐embedded minced cartilage.ConclusionsThis study shows that the mincing of cartilage leads to increased chondrocyte death and decreased expression of chondrocyte phenotypic marker genes after 7 days. The use of collagen 1 gel may improve the stability of the phenotype, which needs to be further elucidated.Level of EvidenceLevel III (therapeutic).
Investigating the impacts of solid phase extraction on dissolved organic matter optical signatures and the pairing with high‐resolution mass spectrometry data across a freshwater stream network
Authors: Roebuck Jr J. Alan , Myers‐Pigg Allison N. , Garayburu‐Caruso Vanessa , Stegen James 
Published: February 2024

AbstractAdvancing our understanding of dissolved organic matter (DOM) chemistry in aquatic systems necessitates the integration of data streams from multiple analytical platforms. Some measurements require pretreatment with solid phase extraction (SPE), while others are performed directly on whole water samples. Evidence has suggested that SPE will be biased against select DOM fractions, leading to concerns over the ability to establish data linkages across platforms with variable needs for SPE pretreatment, such as those from optical measurements and those that provide high‐resolution molecular information. Here, we directly addressed this concern by assessing the impact of SPE on DOM optical properties through excitation–emission matrices with parallel factor analysis (PARAFAC) for 47 samples across a stream network within a single watershed reflective of variable DOM sources. PARAFAC data was further paired with molecular information obtained by Fourier transform ion cyclotron resonance mass spectrometry (FTICR‐MS). A comparison of PARAFAC models first revealed no systematic qualitative differences in major components between whole water DOM and DOM isolated by SPE (SPE‐DOM); however, quantitative biases against select components were observed. Further linkages with FTICR‐MS data revealed that the molecular fingerprint associated with each PARAFAC component was consistent between the whole water DOM and SPE‐DOM. Our results suggest that bulk scale linkages across these analytical platforms could be inferred irrespective of the observed quantitative biases resulting from SPE for samples within this example watershed. This work represents a key step toward the systematic evaluation of linkages between optical and high‐resolution mass spectrometry datasets in freshwater lotic environments.
Reduction of Pro‐Inflammatory Markers in RAW264.7 Macrophages by Polyethylenimines
Authors: Frisch Emilie , Dussouillez Candice , McCartin Conor , Blumberger Juliette , Humbert Chana , Lebeau Luc , Frisch Benoît , Heurtault Béatrice , Kichler Antoine , Fournel Sylvie 
Published: February 2024

AbstractThe physiological problem of chronic inflammation and its associated pathologies attract ongoing attention with regard to methods for their control. Current systemic pharmacological treatments present problematic side effects. Thus, the possibility of new anti‐inflammatory compounds with differing mechanisms of action or biophysical properties is enticing. Cationic polymers, with their ability to act as carriers for other molecules or to form bio‐compatible materials, present one such possibility. Although not well described, several polycations such as chitosan and polyarginine, have displayed anti‐inflammatory properties. The present work shows the ubiquitous laboratory transfection reagent, polyethylenimine (PEI) and more specifically low molecular weight branched PEI (B‐PEI) as also possessing such properties. Using a RAW264.7 murine cell line macrophage as an inflammation model, it is found the B‐PEI 700 Da as being capable of reducing the production of several pro‐inflammatory molecules induced by the endotoxin lipopolysaccharide. Although further studies are required for elucidation of its mechanisms, the revelation that such a common lab reagent may present these effects has wide‐ranging implications, as well as an abundance of possibilities.
Extension and embedding theorems for Campanato spaces on C0,γ$C^{0,\gamma }$ domains
Authors: Greco Damiano , Lamberti Pier Domenico 
Published: February 2024

AbstractWe consider Campanato spaces with exponents on domains of class in the N‐dimensional Euclidean space endowed with a natural anisotropic metric depending on . We discuss several results including the appropriate Campanato's embedding theorem and we prove that functions of those spaces can be extended to the whole of the Euclidean space without deterioration of the exponents .
Multi‐omics analysis of disulfidptosis regulators and therapeutic potential reveals glycogen synthase 1 as a disulfidptosis triggering target for triple‐negative breast cancer
Authors: Xie Jindong , Deng Xinpei , Xie Yi , Zhu Hongbo , Liu Peng , Deng Wei , Ning Li , Tang Yuhui , Sun Yuying , Tang Hailin , Cai Manbo , Xie Xiaoming , Zou Yutian 
Published: February 2024

AbstractDisruption of disulfide homeostasis during biological processes can have fatal consequences. Excess disulfides induce cell death in a novel manner, termed as “disulfidptosis.” However, the specific mechanism of disulfidptosis has not yet been elucidated. To determine the cancer types sensitive to disulfidptosis and outline the corresponding treatment strategies, we firstly investigated the crucial functions of disulfidptosis regulators pan‐cancer at multi‐omics levels. We found that different tumor types expressed dysregulated levels of disulfidptosis regulators, most of which had an impact on tumor prognosis. Moreover, we calculated the disulfidptosis activity score in tumors and validated it using multiple independent datasets. Additionally, we found that disulfidptosis activity was correlated with classic biological processes and pathways in various cancers. Disulfidptosis activity was also associated with tumor immune characteristics and could predict immunotherapy outcomes. Notably, the disulfidptosis regulator, glycogen synthase 1 (GYS1), was identified as a promising target for triple‐negative breast cancer and validated via in vitro and in vivo experiments. In conclusion, our study elucidated the complex molecular phenotypes and clinicopathological correlations of disulfidptosis regulators in tumors, laying a solid foundation for the development of disulfidptosis‐targeting strategies for cancer treatment.
Effect of annealing on the morphology and properties of poly(lactic acid)/polyhedral oligomeric silsesquioxane asymmetric porous membranes prepared through non‐solvent induced phase separation and its application
Authors: Ramachandran Jeeshma , Thomas Selvin P. , Thomas Sabu , Stephen Ranimol 
Published: February 2024

AbstractNon‐solvent induced phase separation (NIPS) method was employed to fabricate biodegradable poly(lactic acid) (PLA) nanocomposite membranes. Morphological studies using scanning electron microscopy revealed that all the membranes prepared display asymmetric structures comprising finger‐like macropores. The incorporation of modified polyhedral oligomeric silsesquioxane (POSS) particles into the PLA matrix resulted in enhanced crystallinity, mechanical, and thermal properties. Annealing of the membranes was performed to explore the influence of temperature on the morphology and properties. After annealing, membranes become more thin and compact, and drastic enhancement in crystallinity is also observed. Consequently, Young's modulus experiences a significant improvement. The reduction in oil absorption capacity after annealing can be attributed to the higher level of crystallinity, reduced porosity, and smaller pore diameter observed in the annealed membranes. Additionally, the unannealed PLA nanocomposite membranes demonstrated exceptional oil absorption capacity, reaching approximately 88%. It is foreseeable that these PLA/POSS nanocomposite membranes possess the potential to be utilized as effective tools for oil–water separation, offering the advantage of mitigating secondary pollution.
Designing magnetic catalysts based on gold nanoparticles supported by ethylenediamine tetraacetic acid functionalized amino‐modified poly(N‐isopropyl acrylamide) for reduction of nitro compounds in water
Authors: Kafshboran Hadieh Rahbar , Ghasemi Soheila 
Published: February 2024

AbstractA novel smart catalyst was developed with amino‐modified thermo‐responsive poly(N‐isopropyl acrylamide) (PNIPAM) grafts on silica‐coated magnetic nanoparticles using the conventional free radical polymerization process. By this methodology, the polymer was successfully grafted mainly onto silica‐modified iron oxide nanoparticles (Fe3O4@Si). The PNIPAM‐grafted Fe3O4@Si was then subjected to ethylenediamine treatment to produce the amino‐functionalized support (Fe3O4@Si@PNIPAM‐NH2), which was subsequently modified with carboxyl functional groups by using EDTA (ethylenediaminetetraacetic acid) to create Fe3O4@Si@PNIPAM‐NH2‐EDTA. Au nanoparticles were then decorated on this support through its two amines and four carboxylates. Different methods were used to study this novel catalyst, including inductively coupled plasma, Fourier transport infrared spectroscopy, TGA, dynamic light scattering, SEM, energy‐dispersive X‐ray analysis, vibrating sample magnetometry, XRD and elemental CHN analysis. The reduction of several aryl‐nitro derivatives demonstrated a significant catalytic activity for the as‐synthesized gold catalyst (Fe3O4@Si@PNIPAM‐NH2‐EDTA‐Au). The Au catalyst can be successfully removed from the reaction components using a magnetic field and used again in eight successive reduction reactions without significant gold leaching and loss of catalytic activity. © 2024 Society of Industrial Chemistry.
Clinical analysis of 94 cases of ocular adnexal lymphomas
Authors: Sun Yu‐ying , Luo Shu‐ai , Huang Chun‐yu 
Published: February 2024

AbstractTo investigate the imaging manifestations and pathological features of ocular adnexal lymphoma (OALs) in order to provide relevant information for ophthalmologists. A total of 94 patients with OALs were pathologically confirmed between August 2018 and July 2023, and their demographics, location, subtype, treatment, and prognosis were retrospectively studied. 94 patients with OALs were included in this study, of whom 28 were female (29.79%) and 66 were male (70.21%). The age of the patients ranged from 21 to 80 years, with a predominance of 50–60 years (60/94); monocular onset was common (90/94), and the most site of onset was orbital (74/94), followed by conjunctiva (12/94), eyelids (4/94), vitreous (3/94), and lacrimal gland (1/94). CT and MRI can show the involvement and extent of the lesions, and the pathology of the disease is characterized by mucosa‐associated lymphoid tissue marginal zone B‐cell lymphoma (72/94), diffuse large B‐cell lymphoma (10/94), T‐cell lymphoma (4/94), mantle cell lymphoma (2/94), Burkitt's lymphoma (2/94), small lymphocytic lymphoma (2/94), and follicular lymphoma (1/94). All patients received surgical treatment, 42 patients received local radiation therapy, 35 patients received chemotherapy, 13 patients received radiation therapy and chemotherapy successively, and 3 patients received comprehensive treatment. During the follow‐up, three patients relapsed, four died, one was lost to follow‐up, and the remaining patients had stable conditions. The majority of OALs are mucosa‐associated lymphoid tissue marginal zone B‐cell lymphomas (MALT) with an excellent prognosis. It mostly occurs in people over 50 years of age and most site of the onset is orbital.
Testosterone bounce predicts favorable prognoses for prostate cancer patients treated with degarelix
Authors: Kamada Shuhei , Sakamoto Shinichi , Kinoshita Ryo , Zhao Xue , Kamasako Tomohiko , Yamase Ryosuke , Junryo Rii , Saito Shinpei , Sangjon Pae , Takei Akinori , Yamada Yasutaka , Goto Yusuke , Imamura Yusuke , Iguchi Taro , Mizokami Atsushi , Suzuki Hiroyoshi , Akakura Koichiro , Ichikawa Tomohiko 
Published: February 2024

AbstractBackgroundTo clarify the clinical roles of changes in testosterone (T) levels with a cut‐off level of 20 ng/dL as predictive factors for prostate cancer patients treated with degarelix acetate.MethodsA total of 120 prostate cancer patients who received hormone therapies with gonadotropin‐releasing hormone antagonist degarelix acetate were retrospectively analyzed. The predictive values of nadir T levels, max T levels, T bounce, and other clinical factors were evaluated for overall survival (OS), cancer‐specific survival (CSS), and progression‐free survival (PFS). T bounce was defined as satisfying both nadir serum T levels of <20 ng/dL and max serum T levels of ≥20 ng/dL during hormone therapies.ResultsIn 120 prostate cancer patients, 16 (13%) patients did not achieve nadir T < 20 ng/dL, and 76 (63%) patients had max T ≥ 20 ng/dL. The median times to nadir T and max T are 108 and 312 days, respectively. T bounce was shown in 60 (50%) patients and is associated with favorable prognoses both for OS (p = 0.0019) and CSS (p = 0.0013) but not for PFS (p = 0.92). While in the subgroup analyses of the patients with the progression of the first‐line hormone therapies, T bounce predicts favorable OS (p = 0.0015) and CSS (p = 0.0013) after biochemical recurrence.ConclusionsThe present study revealed that T bounce with cut‐off levels of 20 ng/dL is a promising biomarker that predicts OS and CSS for prostate cancer patients treated with degarelix acetate.
Advances in making Mn oxide‐coated sands
Authors: Rabenhorst Martin C. , Wardrup Jocelyn L. 
Published: February 2024

AbstractManganese (Mn) oxide‐coated sand has been suggested as an amendment for scrubbing metals in water filtration beds and also as a less concentrated medium for uniformly amending soils with Mn oxides in mesocosm scale studies. Earlier work at the lab bench scale, using potassium permanganate (KMnO4) solutions that were reduced with sodium (Na) lactate, resulted in sands coated with about 0.13% Mn. The goal of this project was to increase the amount of Mn oxide that could be coated on sand to make it a more useful amendment and also to attempt to scale up the procedure to produce larger (kg) quantities of coated sand. Titration experiments examined the effects of (1) varying the molar ratio of Na lactate to KMnO4, (2) varying the rate at which the titration was accomplished, and (3) varying the concentration (molarity) of the original KMnO4 solution. The results of this work led to an optimal approach utilizing 0.32 M KMnO4 solution that was titrated to a final lactate:permanganate ratio of ∼1.1 with 10% of the lactate being added every 10 min while the suspension was being stirred. The proportion of sand to an initial solution was also increased 5–20 fold to between 50 and 200 g per 100 mL of solution. Applying this method and using a large 20‐ to 30‐L reaction vessel yields sands coated with up to 0.7% Mn in batches 5–10 kg is size, which could be useful as an amendment in mesocosm scale studies, or as a component of treatment filter beds. The examination of various size fractions of the coated sands demonstrated that more Mn was coated on finer sand fractions, which appears to be a function of the particle surface area available for the coating of Mn oxides, and at a rate of 0.3–0.5 µg Mn mm−2 of the particle surface.
Microbially Inspired Calcium Carbonate Precipitation Pathway Integrated Polyelectrolyte Capsules (MICPC) for Biomolecules Release
Authors: Yuan Hao , Jia Lufan , Xie Xin , Li Qinyuan , Peng Yali , Ma Qingming , Guo Ting , Meng Tao 
Published: February 2024

AbstractComplexation between oppositely charged polyelectrolytes offers a facile single‐step strategy for assembling functional micro‐nano carriers for efficient drug and vaccine delivery. However, the stability of the delivery system within the physiological environment is compromised due to the swelling of the polyelectrolyte complex, driven by the charge shielding effect, and consequently leads to uncontrollable burst release, thereby limiting its potential applications. In a pioneering approach, cellular pathway‐inspired calcium carbonate precipitation pathways are developed that are integrated into polyelectrolyte capsules (MICPC). These innovative capsules are fabricated at the interface of all‐aqueous microfluidic droplets, resulting in a precisely controllable and sustained release profile in physiological conditions. Unlike single‐step polyelectrolyte assembly capsules which always perform rapid burst release, the MICPC exhibits a sustainable and tunable release pattern, releasing biomolecules at an average rate of 3−10% per day. Remarkably, the degree of control over MICPC's release kinetics can be finely tuned by adjusting the quantity of synthesized calcium carbonate particles within the polyelectrolyte complex. This groundbreaking work not only deepens the insights into polyelectrolyte complexation but also significantly enhances the overall stability of these complexes, opening up new avenues for expanding the range of applications involving polyelectrolyte complex‐related materials.
Engineering pH‐Responsive, Self‐Healing Vesicle‐Type Artificial Tissues with Higher‐Order Cooperative Functionalities
Authors: Kojima Tomoya , Noguchi Yutaro , Terasaka Koichi , Asakura Kouichi , Banno Taisuke 
Published: February 2024

AbstractMulticellular organisms demonstrate a hierarchical organization where multiple cells collectively form tissues, thereby enabling higher‐order cooperative functionalities beyond the capabilities of individual cells. Drawing inspiration from this biological organization, assemblies of multiple protocells are developed to create novel functional materials with emergent higher‐order cooperative functionalities. This paper presents new artificial tissues derived from multiple vesicles, which serve as protocellular models. These tissues are formed and manipulated through non‐covalent interactions triggered by a salt bridge. Exhibiting pH‐sensitive reversible formation and destruction under neutral conditions, these artificial vesicle tissues demonstrate three distinct higher‐order cooperative functionalities: transportation of large cargoes, photo‐induced contractions, and enhanced survivability against external threats. The rapid assembly and disassembly of these artificial tissues in response to pH variations enable controlled mechanical task performance. Additionally, the self‐healing property of these artificial tissues indicates robustness against external mechanical damage. The research suggests that these vesicles can detect specific pH environments and spontaneously assemble into artificial tissues with advanced functionalities. This leads to the possibility of developing intelligent materials with high environmental specificity, particularly for applications in soft robotics.
Rational Electrochemical Design of Cuprous Oxide Hierarchical Microarchitectures and Their Derivatives for SERS Sensing Applications
Authors: An Ning , Chen Tiantian , Zhang Junfeng , Wang Guanghui , Yan Mi , Yang Shikuan 
Published: February 2024

AbstractRational morphology control of inorganic microarchitectures is important in diverse fields, requiring precise regulation of nucleation and growth processes. While wet chemical methods have achieved success regarding the shape‐controlled synthesis of micro/nanostructures, accurately controlling the growth behavior in real time remains challenging. Comparatively, the electrodeposition technique can immediately control the growth behavior by tuning the overpotential, whereas it is rarely used to design complex microarchitectures. Here, the electrochemical design of complex Cu2O microarchitectures step‐by‐step by precisely controlling the growth behavior is demonstrated. The growth modes can be switched between the thermodynamic and kinetic modes by varying the overpotential. Cl− ions preferably adhered to {100} facets to modulate growth rates of these facets is proved. The discovered growth modes to prepare Cu2O microarchitectures composed of multiple building units inaccessible with existing methods are employed. Polyvinyl alcohol (PVA) additives can guarantee all pre‐electrodeposits simultaneously evolve into uniform microarchitectures, instead of forming undesired microstructures on bare electrode surfaces in following electrodeposition processes is discovered. The designed Cu2O microarchitectures can be converted into noble metal microstructures with shapes unchanged, which can be used as surface‐enhanced Raman scattering substrates. An electrochemical avenue toward rational design of complex inorganic microarchitectures is opened up.
Directed Evolution of Escherichia coli Nissle 1917 to Utilize Allulose as Sole Carbon Source
Authors: Xu Bo , Liu Li‐Hua , Lai Shijing , Chen Jingjing , Wu Song , Lei Wei , Lin Houliang , Zhang Yu , Hu Yucheng , He Jingtao , Chen Xipeng , He Qian , Yang Min , Wang Haimei , Zhao Xuemei , Wang Man , Luo Haodong , Ge Qijun , Gao Huamei , Xia Jiaqi , Cao Zhen , Zhang Baoxun , Jiang Ao , Wu Yi‐Rui 
Published: February 2024

AbstractSugar substitutes are popular due to their akin taste and low calories. However, excessive use of aspartame and erythritol can have varying effects. While D‐allulose is presently deemed a secure alternative to sugar, its excessive consumption is not devoid of cellular stress implications. In this study, the evolution of Escherichia coli Nissle 1917 (EcN) is directed to utilize allulose as sole carbon source through a combination of adaptive laboratory evolution (ALE) and fluorescence‐activated droplet sorting (FADS) techniques. Employing whole genome sequencing (WGS) and clustered regularly interspaced short palindromic repeats interference (CRISPRi) in conjunction with compensatory expression displayed those genetic mutations in sugar and amino acid metabolic pathways, including glnP, glpF, gmpA, nagE, pgmB, ybaN, etc., increased allulose assimilation. Enzyme‐substrate dynamics simulations and deep learning predict enhanced substrate specificity and catalytic efficiency in nagE A247E and pgmB G12R mutants. The findings evince that these mutations hold considerable promise in enhancing allulose uptake and facilitating its conversion into glycolysis, thus signifying the emergence of a novel metabolic pathway for allulose utilization. These revelations bear immense potential for the sustainable utilization of D‐allulose in promoting health and well‐being.