bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Feasibility of Modified Laparoscopic Female Radical Cystectomy: A Procedural Guide and Comparison with Traditional Technique
Authors: chen xin , Jiang Xiao-Lu , Sun Jia-Ning , Yang Rui , Zhang Feng , Sun Wei-Cheng , Wu Ji-Tao , Zhao Hong-Wei 
Published: April 2024

Abstract

Females are generally less likely to develop carcinoma of the urinary bladder compared with melas. Despite radical cystectomy (RC) being an effective surgical technique, its usage in women is limited due to the decreased incidence and potential postoperative complications. We sought to evaluate the safety and feasibility of a novel modified laparoscopic radical cystectomy (MLRC) for female patients with bladder cancer and introduce the modified procedural stepwise. The retrospective cohort study spanned from January 1, 2018, to September 30, 2023, and included 45 female patients who underwent RC. Perioperative data comparison between the MLRC group (25 patients) and the traditional laparoscopic radical cystectomy (TLRC) group (20 patients) was conducted, with differences being deemed significant at P < 0.05. All operations were performed successfully without conversion to open surgery. The MLRC group exhibited less %sCre change (P < 0.001), less estimated blood loss (EBL) (P < 0.001), shorter operative time (P < 0.001), shorter pelvic lymph node dissection (PLND) time (P < 0.001), shorter first postoperative defecation (FPD) time (P < 0.001), and fewer complications (P < 0.05). However, the postoperative hospital stay, urinary reconstruction, overall survival, local recurrence or distant metastasis, and American Society of Anaesthesiology (ASA) score showed no statistical difference. Patients were followed up for 3 to 65 months, with a median of 27 months.MLRC is a safe and feasible procedure that could improve perioperative outcomes compared to traditional procedures.
Experimental research on breakage characteristics of feed pellets under different loading methods
Authors: Kong Xianrui , Cao Qing , Yong Ganwen , Wang Duo , Niu Zhiyou 
Published: April 2024

Abstract
Particle breakage is a common phenomenon during the processing of production, storage, and transportation. Because of the requirements for pellet integrity in poultry farming, research on the breakage characteristics of feed pellets is necessary. In this paper, repeated compression tests under different loading forces and repeated impact tests under different air pressures were carried out with feed pellets as the research object. The breakage behaviors were described, and the particle size distribution of feed pellets was analyzed quantitatively. The variation of energy and pulverization rate under different loading methods was analyzed with the increase of loading cycles. The relationship between energy and pulverization rate was fitted. The results showed that the breakage degree decreased gradually from top to bottom in repeated compression tests, and the crack density positively correlated with the loading force. The size of the feed pellets during repeated impacts decreased continuously and was negatively correlated with air pressure. The Weibull function could fit the particle size distribution well, and the R2 values were greater than 0.97 and 0.96. Weibull parameters showed a steady breakage degree in compression tests and a growing breakage degree in impact tests. The energy and pulverization rate increased with the loading cycles in different loading methods. The model of Vogel and Peukert could describe the relationship between energy and pulverization rate well, and the R2 values were greater than 0.94. The minimum energy required for pellet breakage in compression was greater than that in impact due to the compaction of the feed particle bed under repeated compression. The results can provide basic theory and data support for breakage characteristics and quality evaluation of feed pellets.
HBV-mediated Suppression of Megakaryocyte Differentiation through UBE4B Upregulation and Modulation of p53 and ERK1/2
Authors: Jiang Huinan , Sheng Qiuju , Li Yanwei , Dou Xiaoguang , Ding Yang , Zhang Chong , Han Chao , Fan Yaoxin 
Published: April 2024

Abstract
Background
Hepatitis B virus (HBV) infection can cause thrombocytopenia through its effects on hematopoiesis, but the mechanisms underlying this process are still unclear. The process of platelet generation encompasses multiple stages, among these stages, the differentiation of mature megakaryocytes plays a pivotal role in platelet production.
Objective
In this study, we investigated the impact of HBV on the differentiation of mature megakaryocytes and its molecular mechanism.
Methods
Different doses of HBV virus solution extracted from HepAD38 cells were co-cultured with hematopoietic stem cells isolated from fresh, full-term healthy maternal cord blood through magnetic bead sorting. The study aimed to assess the variances in megakaryocyte differentiation and maturation after HBV infection. Furthermore, Label-free quantitative proteomics was employed to analyze the differential proteins during the mature megakaryocyte stages pre- and post-HBV infection, with a focus on elucidating their respective molecular mechanisms.
Results
Through comparison, it was discovered that HBV could impede the differentiation process of fully developed megakaryocytes. Specifically, during the maturation stage of megakaryocytes, HBV had the ability to hinder cell DNA polyploidization as well as the formation of cytoskeletal proteins. Furthermore, its suppressive effect was more pronounced in the later stages of differentiation, consequently leading to impaired platelet production. Proteomic analysis revealed noteworthy disparities in UBE4B protein levels within mature megakaryocytes after HBV infection. Upon transfection with lentivirus and subsequent knockdown of UBE4B in mature megakaryocytes, a noticeable alleviation of HBV's inhibitory impact on mature megakaryocytes was observed, accompanied by regulation of p53 and ERK1/2 expression and phosphorylation in the cells.
Conclusion
HBV can upregulate the expression of UBE4B, inhibit the expression and phosphorylation of p53, enhance the expression and phosphorylation of ERK1/2, suppress the differentiation of mature megakaryocytes, thereby leading to platelet production disorders.
Evodiamine Inhibits the Progression of Esophageal Aquamous Cell Carcinoma via Modulating PI3K/AKT/mTOR Pathway
Authors: Jiang Hangyu , Yuan Qiang , Chu Yueming , Li Yuqi , Zhu Maofei , Li Lin 
Published: April 2024

Abstract

Purpose
Esophageal squamous cell carcinoma (ESCC) is one of the main reasons of cancer-associated deaths. The effective antitumor drug evodiamine (EVO) is attracting increased, showing suppression of the development of tumor. Nevertheless, the potential mechanisms are not clear. Therefore, exploring promising therapeutic targets and the mechanisms of the anti-tumor effects of EVO is urgent for the treatment of ESCC.
Methods
In this study, two ESCC cell lines, TE-1 and KYSE150 were subjected to cell viability assay. Flow cytometry assays were applied to assess the apoptosis and the distribution of cell cycle. The metastasis capacity of ESCC cell was assessed via wound healing assays and transwell assays. Furthermore, western blot analysis is applied to test the expression of proteins. Finally, ESCC xenograft models were established in vivo.
Results
In the current research, we revealed that EVO triggered caspase-dependent apoptosis and G2/M phase arrest and impaired the migration and invasion, which suppressed the proliferation of ESCC cells. Mechanically, this study demonstrated that EVO could suppress the growth of ESCC cells by regulating PI3K/AKT/mTOR signal pathways. In addition, EVO treatment of mice also attenuated tumor growth in ESCC xenograft models.
Conclusion: The results revealed that EVO induced ESCC cell apoptosis and blocked cell cycle at G2/M phase as well as anti-metastatic through suppressing PI3K/AKT/mTOR signal pathways, indicating EVO could be a potential chemotherapeutic candidate for ESCC.
Investıgatıon of the Effects of Treatment with Enoxaparın Sodıum and Hyperbarıc Oxygen Therapy on the Recovery of Rats Wıth Achılles Tendon Rupture
Authors: Aytekin Cafer Erman , Turhan Yalçın , Karaduman Zekeriya Okan , Arican Mehmet , Sağlam Sönmez , Coşkun Sinem Kantarcıoğlu , Uludağ Veysel 
Published: April 2024

Abstract

Purpose
In this study, we aimed to investigate the effects of hyperbaric oxygen therapy and enoxaparin sodium, which are known to accelerate bone tissue healing as well as tendon and soft tissue healing, on the healing of Achilles tendon rupture.
Methods
Thirty-six rats were used in the present study. All rats were divided into groups of nine. The groups were the enoxaparin sodium group, enoxaparin sodium and hyperbaric oxygen group, hyperbaric oxygen group and control group. After 21 days, the process was completed, and the rats were sacrificed. Achilles tendon samples were evaluated histopathologically.
Results
The groups were compared according to the results of statistical analysis based on the histopathological data. There was no significant difference between the groups in terms of acute inflammation (p = 0.785) or chronic inflammation (p = 0.827) scores, but there were significant differences in neovascularization (p = 0.009), proliferation (p < 0.001) and fibrosis (p = 0.006) scores.
Conclusion
Our study showed that the use of enoxaparin sodium and hyperbaric oxygen had a positive effect on the healing of the Achilles tendon. Based on these results, we believe that the use of enoxaparin sodium and hyperbaric oxygen therapy after Achilles tendon rupture will be beneficial for healing and preventing complications.
Identification of Biomarkers in Intervertebral Disc Degeneration Using Bioinformatics and Machine Learning Algorithms
Authors: Zhang Hao , Shi Shengbo , Huang Xingxing , Gong Changsheng , Zhang Zijing , Zhao Zetian , Gao Junxiao , Zhang Meng , Yu Xiaobing 
Published: April 2024

Abstract

Background
Intervertebral Disc Degeneration (IDD) is a major cause of lower back pain and a significant global health issue. However, the specific mechanisms of IDD remain unclear. This study aims to identify key genes and pathways associated with IDD using bioinformatics and machine learning algorithms.
Methods
Gene expression profiles, including those from 35 LDH patients and 43 healthy volunteers, were downloaded from the GEO database (GSE124272, GSE150408, GSE23130, GSE153761). After merging four microarray datasets, differentially expressed genes (DEGs) were selected for GO and KEGG pathway enrichment analysis. Weighted Gene Co-expression Network Analysis (WGCNA) was then applied to the merged dataset to identify relevant modules and intersect with DEGs to discover candidate genes with diagnostic value. A LASSO model was established to select appropriate genes, and ROC curves were drawn to elucidate the diagnostic value of genetic markers. A Protein-Protein Interaction (PPI) network was constructed and visualized to determine central genes, followed by external validation using qRT-PCR.
Results
Differential analysis of the preprocessed dataset identified 244 genes, including 183 upregulated and 61 downregulated genes. WGCNA analysis revealed the most relevant module intersecting with DEGs, yielding 9 candidate genes. The lasso-cox method was used for regression analysis, ultimately identifying 6 genes: ASPH, CDC42EP3, FOSL2, IL1R1, NFKBIZ, TCF7L2. A Protein-Protein Interaction (PPI) network created with GENEMANIA identified IL1R1 and TCF7L2 as central genes.
Conclusion
Our study indicates that IL1R1 and TCF7L2 are potential biomarkers for IDD, offering new insights into the pathogenesis and therapeutic development of IDD.
Psychiatric outpatient care: work of general and specialized hospital settings in Baghdad: Insight from Iraq
Authors: Younis Maha Sulaiman , Saeed Amina Jassim , Al-Badri Sajjad G. , Hashim Hashim Talib 
Published: April 2024

Abstract

Background
The establishment of psychiatric services in general hospitals has been driven by various factors, including the prioritization of communicable and non-communicable diseases, the de-institutionalization movement in mental health care, and the geographic and sociocultural barriers to accessing specialized psychiatric services. In Iraq, the stigmatization of mental illness and the under-provision of community-based facilities contribute to the underreporting of mental disorders and the inclination to seek care from non-specialist physicians. This study aims to evaluate the demographic characteristics, patterns of psychiatric disorders, referral and consultation practices, and patient satisfaction in general and specialized psychiatric outpatient settings in Iraq.
Methods
A prospective cross-sectional study was conducted with 650 patients attending outpatient psychiatric clinics at Baghdad Teaching Hospital and Ibn-Rushd (IRH) Psychiatric Hospital from February to July 2021. Data were collected through semi-structured interviews based on DSM-5 criteria and analyzed using Statistical Package for Social Science (SPSS) version 27.
Results
Attendees predominantly presented with major depressive disorder, schizophrenia, and generalized anxiety disorder. Patterns of consultation varied between the two hospitals, with IRH showing a higher prevalence of schizophrenia and chronic cases, while Baghdad Hospital had a higher proportion of first-time visitors. Patient satisfaction was generally higher in the specialized IRH setting compared to the general hospital. The workload was significantly heavier in IRH, reflecting the preference for specialized psychiatric care. The study highlighted the challenges of the mental health system in Iraq, including the impact of political instability, conflict, and underinvestment in healthcare infrastructure.
Conclusion
The study underscores the necessity of enhancing psychiatric services in general hospitals in Iraq to cater to the diverse needs of patients with mental disorders. It emphasizes the importance of addressing the sociocultural stigma and the structural challenges faced by the healthcare system. Integrating psychiatric services within general hospitals could improve accessibility and reduce the burden on specialized facilities, provided that the quality of care and patient satisfaction are ensured.
Geological evidences: New insights into rock avalanche dynamics
Authors: Wang Yufeng , Chen Mei , Liu Yuanyuan , Wünnemann Bernd , Cheng Qiangong , Ming Jie , Hu Liwen , Li Kun , Lin Qiwen 
Published: April 2024

Abstract

Rock avalanche (RA) is one of the most enigmatic geological processes on Earth and other planets featured by extremely high mobility1-7. Although great work has been conducted with many hypotheses being proposed8-19, the mechanism for its high mobility remains a challenging issue due to lacking geological evidence20-21. Microscope observations and mineral analysis of the basal and body facies of the Nyixoi Chongco RA on the Tibetan Plateau, China show that grains in the RA deform by brittle fracturing with a frictional temperature being 270 ~ 300 ℃. The basal facies is featured by a dilated regime with a high fragmentation degree, but the body facies displays a dense regime with a relatively low fragmentation although their temperatures are near. Jigsaw structure featured by intensive collision is observed in the basal facies but lacking in the body facies. With these geological evidences, it is reached that the frictional temperature of most RAs featured by brittle fracturing should be low that can not cause the occurrence of thermal decomposition or melt. A mechanism liking a leidenfrost effect should occur in the basal facies, which is related to landquake and dominates the high mobility of RAs by the generation of a relatively dilated regime with the main mass propagated as a cataclastic flow.
Clinical value of Tc-99m MIBI scintigraphy for the level of lower limb amputation in patients with diabetic foot ulcers
Authors: ekici mehmet , günay ali eray , karaçavuş seyhan , gençer hümeyra , ozan fırat 
Published: April 2024

Abstract

Background
There is a positive relationship between mitochondrial damage in the cell and uptake in 99mTc-MIBI scintigraphy. Severe mitochondrial dysfunction with cell death occurs in patients with diabetic foot ulcers. 99mTc-MIBI scintigraphy should be considered in order to decide on the level of amputation.
Methods
Prospectively twenty-four patients with diabetic foot ulcers (DFUs) were included in the study. As a result of treatment that started with the hospitalization, patients whose DFUs healed and did not need surgical intervention were determined as group 1, and patients whose DFUs did not regress despite surgical and medical treatment and who required further surgical intervention were determined as Group 2. 99mTc-MIBI scintigraphy was obtained before surgery. The 99mTc-MIBI uptake rates of the injured foot relative to the healthy foot were recorded. Deep tissue culture was taken at surgery. ESR, WBC, CRP and albumin values of the patients were measured.
Results
In this study, 99mTc-MIBI uptake rates of patients with poor prognosis were higher at all times than in patients who did not require revision surgery. A significant difference was found between these values in the 10th- and 30th-second rates. While the CRP level was 86.04 ± 21.87 mg / dL in Group 1, it was measured as 144.43 ± 27.54 mg / dL in Group 2 (p = 0,040). There is a positive correlation between ulcerated foot / healthy foot 99mTc-MIBI involvement rates at 10 and 30 seconds and CRP values, and a negative correlation between albumin values.
Conclusion
There is a significant relationship between 99mTc-MIBI involvement rates and poor prognosis and reamputation. The correlation between CRP and albumin levels which are among the predictive values, and 99mTc-MIBI uptake confirms this relationship. In diabetic foot ulcers, which are difficult to management and treatment.
Intraarticular Nodular Fasciitis of the Elbow Confirmed by USP6-MYH9 Gene Fusion.
Authors: Adachi Shiro , Kukita Yoji , Abe Shingo , Nakamura Harumi , Tamura Hiromi , Doi Reiko , Kuriyama Kohji 
Published: April 2024

Abstract

Background: Nodular fasciitis is a benign, usually self-limiting myofibroblastic proliferation with a predilection for the upper extremities, trunk, and head and neck of young adults. Although it usually arises within subcutaneous tissues, it may also occur within skeletal muscle, dermis, vessels, and peripheral nerves. Since nodular fasciitis is not widely recognized to arise within joints, it may therefore cause diagnostic confusion in this uncommon setting. Histology can vary considerably and can, therefore, mimic other benign or low grade malignant neoplasms. Recent molecular analysis revealed that most cases of nodular fasciitis involve USP6-MYH9fusion.
Case presentation: We report an unusual case of an 11-year-old patient who presented with a 6-month history of right elbow swelling and pain. Radiological examinations suggested tenosynovial giant cell tumor. Histology revealed that the tumor was composed solely of proliferating bland spindle cells with osteoclast-like giant cells and scattered foam cells. There was only a focal area of immature granulation-like myxoid change, which suggested nodular fasciitis. Molecular analysis using next-generation sequencing revealed gene rearrangementinvolving USP6 and MYH9, supporting the diagnosis of nodular fasciitis within the elbow joint.
Conclusion: This is the first case of intraarticular nodular fasciitis within the elbow joint in which MYH9-USP6 gene fusion was identified by molecular analysis. Nodular fasciitis should be considered in the differential diagnosis of intraarticular lesions.
Short-Term Outcomes of Endoscopic Ultrasound-Guided Pancreatic Cyst Ablation: A Systematic Review and Meta-Analysis
Authors: Qady Ahmed Al , Nayar Kapil Dev , Emran Joseph , Beirat Amir , Menakuru Sasmith , Harris Dana , Echols Dan J. , Ji Baoan , DeWitt John M , Wang Zhen , Stancampiano Fernando F. , Bi Yan 
Published: April 2024

Abstract

Background
Pancreatic cysts (PC) are increasingly detected through abdominal imaging, prompting exploration of alternatives such as endoscopic ultrasound-guided pancreatic cyst ablation (EUS-PCA) due to the risks and costs associated with surgery. This study conducts a systematic review and meta-analysis of EUS-PCA's short-term efficacy and complications for PC management.
Methods
A systematic review and meta-analysis were carried out on PubMed, Ovid, Cochrane, and TRIP electronic databases. The primary outcome was cyst resolution (partial and complete) and persistence on imaging 12 months after ablation. The secondary outcome was procedure-related adverse events.
Results
Eight studies were eligible for analysis. Complete cyst resolution on imaging 12 months after EUS ablation was 50% [95% CI 36, 63, I2 = 85.31%]. Partial cyst resolution was 27% [95% CI 15, 41, I2 = 87.07%], and cyst persistence was 17% [95% CI 11, 24, I2 = 62.11%]. The rate of complete resolution varied depending on the treatment agent (for ethanol 29% [95% CI 10, 53], lauromacrogol 51% [95% Cl 36, 67], ethanol and paclitaxel 63% [95% CI 48, 76], paclitaxel and gemcitabine 67% [95% CI 45, 83], ethanol, paclitaxel and gemcitabine 61% [95% CI 39, 80]). Post-procedure adverse events included abdominal pain in 4% [95% CI 0, 11], pancreatitis in 3% [95% CI 1, 5], and fever in 1% [95% CI 0, 3] of all patients.
Conclusion
The treatment of pancreatic cysts with EUS ablation results in acceptable levels of complete resolution, and low incidence of severe adverse events. The effectiveness of this treatment is further enhanced when chemoablative agents are employed.
Does having shorter tibias increase the likelihood of developing knee osteoarthritis? An observational cross-sectional radiographic analysis
Authors: Shi Xiaofeng , Lu Yibin , Chen Hongyu , Jia Xinlin , Mao Yuanqing , Zhang Jingwei 
Published: April 2024

Abstract
Background
The crural index, a fundamental property of the human skeletal system, is the ratio of the total length of the tibia to that of the femur. This study aimed to investigate the correlation between the crural index and knee osteoarthritis (OA).
Methods
This observational cross-sectional study included patients with preoperative weight-bearing full-length radiographs who were divided into two groups according to the Kellgren–Lawrence grading system. After rigorous screening based on the inclusion and exclusion criteria, 92 and 125 patients were included in the control and knee OA groups, respectively. The crural index, hip-knee-ankle angle (HKA), the deviation of the HKA from 180° (|HKA-180|), mechanical axis deflection (MAD), mechanical lateral distal femoral angle (mLDFA), medial proximal tibial angle (MPTA), arithmetic HKA (aHKA), and |aHKA| were compared between the two groups. In addition, linear regressions of the crural index were performed to assess their correlations.
Results
The crural index was significantly smaller in the knee OA group than in the control group (crural index: 79.49 ± 2.39 versus 82.14 ± 2.22, respectively; p < 0.001). The crural index correlated well with both |HKA-180| (R² = 0.54, p < 0.05) and MAD (R² = 0.57, p < 0.05).
Conclusions
Patients with OA of the knee have a much smaller crural index, which was closely related to the knee alignment.