bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anatomy and Decadal Evolution of the Pacific Subtropical–Tropical Cells (STCs)*
Authors: Capotondi Antonietta , Alexander Michael A. , Deser Clara , McPhaden Michael J. 
Published: October 2005

Abstract
The output from an ocean general circulation model driven by observed surface forcing (1958–97) is used to examine the evolution and relative timing of the different branches of the Pacific Subtropical–Tropical Cells (STCs) at both interannual and decadal time scales, with emphasis on the 1976–77 climate shift. The STCs consist of equatorward pycnocline transports in the ocean interior and in the western boundary current, equatorial upwelling, and poleward flow in the surface Ekman layer. The interior pycnocline transports exhibit a decreasing trend after the mid-1970s, in agreement with observational transport estimates, and are largely anticorrelated with both the Ekman transports and the boundary current transports at the same latitudes. The boundary current changes tend to compensate for the interior changes at both interannual and decadal time scales. The meridional transport convergence across 9°S and 9°N as well as the equatorial upwelling are strongly correlated with the changes in sea surface temperature (SST) in the central and eastern equatorial Pacific. However, meridional transport variations do not occur simultaneously at each longitude, so that to understand the phase relationship between transport and SST variations it is important to consider the baroclinic ocean adjustment through westward-propagating Rossby waves. The anticorrelation between boundary current changes and interior transport changes can also be understood in terms of the baroclinic adjustment process. In this simulation, the pycnocline transport variations appear to be primarily confined within the Tropics, with maxima around 10°S and 13°N, and related to the local wind forcing; a somewhat different perspective from previous studies that have emphasized the role of wind variations in the subtropics.
Modernisation of Sri Lanka's Traditional Irrigation Systems and Sustainability*
Authors: Zubair Lareef 
Published: October 2005

Arnold Toynbee described the ancient irrigation system of Sri Lanka as an amazing system of irrigated agriculture, which developed over a millennium. It was partially transformed during its encounter with British colonialism with new values, technological systems, administrative structures and a legal code. After the independence in 1948, this transformation continued with foreign aid and technical expertise from the West. The shortcomings of modern irrigation systems with respect to planning, policy and sustainability are now recognised (WCDD 2000). Here, it is argued that the indigenous irrigation systems in Sri Lanka offer a useful counterpoint of one that has been sustained and had many favourable attributes. There are lessons to be learnt from contrasting different modes of irrigation practice in the light of sustainability.
A confocal microscopic study of solitary pulmonary neuroendocrine cells in human airway epithelium
Authors: Weichselbaum Markus , Sparrow Malcolm P , Hamilton Elisha J , Thompson Philip J , Knight Darryl A 
Published: October 2005

Abstract

Background
Pulmonary neuroendocrine cells (PNEC) are specialized epithelial cells that are thought to play important roles in lung development and airway function. PNEC occur either singly or in clusters called neuroepithelial bodies. Our aim was to characterize the three dimensional morphology of PNEC, their distribution, and their relationship to the epithelial nerves in whole mounts of adult human bronchi using confocal microscopy.

Methods
Bronchi were resected from non-diseased portions of a lobe of human lung obtained from 8 thoracotomy patients (Table 1) undergoing surgery for the removal of lung tumors. Whole mounts were stained with antibodies to reveal all nerves (PGP 9.5), sensory nerves (calcitonin gene related peptide, CGRP), and PNEC (PGP 9.5, CGRP and gastrin releasing peptide, GRP). The analysis and rendition of the resulting three-dimensional data sets, including side-projections, was performed using NIH-Image software. Images were colorized and super-imposed using Adobe Photoshop.

Results
PNEC were abundant but not homogenously distributed within the epithelium, with densities ranging from 65/mm2 to denser patches of 250/mm2, depending on the individual wholemount. Rotation of 3-D images revealed a complex morphology; flask-like with the cell body near the basement membrane and a thick stem extending to the lumen. Long processes issued laterally from its base, some lumenal and others with feet-like processes. Calcitonin gene-related peptide (CGRP) was present in about 20% of PNEC, mainly in the processes. CGRP-positive nerves were sparse, with some associated with the apical part of the PNEC.

Conclusion
Our 3D-data demonstrates that PNEC are numerous and exhibit a heterogeneous peptide content suggesting an active and diverse PNEC population.
Extension of Lander-Waterman theory for sequencing filtered DNA libraries
Authors: Wendl Michael C , Barbazuk W Brad 
Published: October 2005

Abstract
Background
The degree to which conventional DNA sequencing techniques will be successful for highly repetitive genomes is unclear. Investigators are therefore considering various filtering methods to select against high-copy sequence in DNA clone libraries. The standard model for random sequencing, Lander-Waterman theory, does not account for two important issues in such libraries, discontinuities and position-based sampling biases (the so-called "edge effect"). We report an extension of the theory for analyzing such configurations.

Results
The edge effect cannot be neglected in most cases. Specifically, rates of coverage and gap reduction are appreciably lower than those for conventional libraries, as predicted by standard theory. Performance decreases as read length increases relative to island size. Although opposite of what happens in a conventional library, this apparent paradox is readily explained in terms of the edge effect. The model agrees well with prototype gene-tagging experiments for Zea mays and Sorghum bicolor. Moreover, the associated density function suggests well-defined probabilistic milestones for the number of reads necessary to capture a given fraction of the gene space. An exception for applying standard theory arises if sequence redundancy is less than about 1-fold. Here, evolution of the random quantities is independent of library gaps and edge effects. This observation effectively validates the practice of using standard theory to estimate the genic enrichment of a library based on light shotgun sequencing.

Conclusion
Coverage performance using a filtered library is significantly lower than that for an equivalent-sized conventional library, suggesting that directed methods may be more critical for the former. The proposed model should be useful for analyzing future projects.
Assessing immunization data quality from routine reports in Mozambique
Authors: Mavimbe João C , Braa Jørn , Bjune Gunnar 
Published: October 2005

Abstract

Background
Worldwide immunization coverage shows an increase in the past years but the validity of the official reports for measuring change over time has been questioned. Facing this problem, donor supported initiatives like the Global Alliance for Vaccine and Immunizations, have been putting a lot of effort into assessing the quality of data used, since accurate immunization information is essential for the Expanded Program on Immunization managers to track and improve program performance. The present article, discusses the practices on record keeping, reporting and the support mechanism to ensure data quality in Mozambique.

Methods
A process evaluation study was carried out in Mozambique in one district (Cuamba) in Niassa Province, between January and March 2003. The study was based on semi-structured interviews, participant observation and review of the data collection materials.

Results
Differences were found for all vaccine types when comparing facility reports with the tally sheets. The same applies when comparing facility reports with district reports. The study also showed that a routine practice during supervision visits was data quality assessment for the outpatient services but none related to data consistency between the tally sheets and the facility report. For the Expanded Program on Immunization, supervisors concentrated more on the consistency checks between data in the facility reports and the number of vaccines received during the same period. Meetings were based on criticism, for example, why health workers did not reach the target. Nothing in terms of data quality was addressed nor validation rules.

Conclusion
In this paper we have argued that the quality of data, and consequently of the information system, must be seen in a broader perspective not focusing only on technicalities (data collection tools and the reporting system) but also on support mechanisms. Implications of a poor data quality system will be reflected in the efficiency of health services facing increased demands, with stagnant or decreasing resources.
Hepatocyte and keratinocyte growth factors and their receptors in human lung emphysema
Authors: Bonay Marcel , Boutten Anne , Leçon-Malas Véronique , Marchal Joëlle , Soler Paul , Fournier Michel , Leseche Guy , Dehoux Monique , Crestani Bruno 
Published: October 2005

Abstract

Background
Hepatocyte and keratinocyte growth factors are key growth factors in the process of alveolar repair. We hypothesized that excessive alveolar destruction observed in lung emphysema involves impaired expression of hepatocyte and keratinocyte growth factors or their respective receptors, c-met and keratinocyte growth factor receptor. The aim of our study was to compare the expression of hepatocyte and keratinocyte growth factors and their receptors in lung samples from 3 groups of patients: emphysema; smokers without emphysema and non-smokers without emphysema.

Methods
Hepatocyte and keratinocyte growth factor proteins were analysed by immunoassay and western blot; mRNA expression was measured by real time quantitative polymerase chain reaction.

Results
Hepatocyte and keratinocyte growth factors, c-met and keratinocyte growth factor receptor mRNA levels were similar in emphysema and non-emphysema patients. Hepatocyte growth factor mRNA correlated negatively with FEV1 and the FEV1/FVC ratio both in emphysema patients and in smokers with or without emphysema. Hepatocyte and keratinocyte growth factor protein concentrations were similar in all patients' groups.

Conclusion
The expression of hepatocyte and keratinocyte growth factors and their receptors is preserved in patients with lung emphysema as compared to patients without emphysema. Hepatocyte growth factor mRNA correlates with the severity of airflow obstruction in smokers.
Does a pre-hospital emergency pathway improve early diagnosis and referral in suspected stroke patients? – Study protocol of a cluster randomised trial [ISRCTN41456865]
Authors: Ferri Marica , De Luca Assunta , Rossi Paolo Giorgi , Lori Giuliano , Guasticchi Gabriella 
Published: October 2005

Abstract

Background
Early interventions proved to be able to improve prognosis in acute stroke patients. Prompt identification of symptoms, organised timely and efficient transportation towards appropriate facilities, become essential part of effective treatment. The implementation of an evidence based pre-hospital stroke care pathway may be a method for achieving the organizational standards required to grant appropriate care. We performed a systematic search for studies evaluating the effect of pre-hospital and emergency interventions for suspected stroke patients and we found that there seems to be only a few studies on the emergency field and none about implementation of clinical pathways.
We will test the hypothesis that the adoption of emergency clinical pathway improves early diagnosis and referral in suspected stroke patients. We designed a cluster randomised controlled trial (C-RCT), the most powerful study design to assess the impact of complex interventions. The study was registered in the Current Controlled Trials Register: ISRCTN41456865 – Implementation of pre-hospital emergency pathway for stroke – a cluster randomised trial.

Methods/design
Two-arm cluster-randomised trial (C-RCT). 16 emergency services and 14 emergency rooms were randomised either to arm 1 (comprising a training module and administration of the guideline), or to arm 2 (no intervention, current practice). Arm 1 participants (152 physicians, 280 nurses, 50 drivers) attended an interactive two sessions course with continuous medical education CME credits on the contents of the clinical pathway. We estimated that around 750 patients will be met by the services in the 6 months of observation. This duration allows recruiting a sample of patients sufficient to observe a 30% improvement in the proportion of appropriate diagnoses.
Data collection will be performed using current information systems. Process outcomes will be measured at the cluster level six months after the intervention. We will assess the guideline recommendations for emergency and pre-hospital stroke management relative to: 1) promptness of interventions for hyperacute ischaemic stroke; 2) promptness of interventions for hyperacute haemorrhagic stroke 3) appropriate diagnosis. Outcomes will be expressed as proportions of patients with a positive CT for ischaemic stroke and symptoms onset <= 6 hour admitted to the stroke unit.

Discussion
The fields in which this trial will play are usually neglected by Randomised Controlled Trial (RCT). We have chosen the Cluster-randomised Controlled Trial (C-RCT) to address the issues of contamination, adherence to real practice, and community dimension of the intervention, with a complex definition of clusters and an extensive use of routine data to collect the outcomes.
The effects of injection of bovine vaccine into a human digit: a case report
Authors: O'Neill Jennifer K , Richards Simon W , Ricketts David M , Patterson Marc H 
Published: October 2005

Abstract

Background
The incidence of needlestick injuries in farmers and veterinary surgeons is significant and the consequences of such an injection can be serious.

Case presentation
We report accidental injection of bovine vaccine into the base of the little finger. This resulted in increased pressure in the flexor sheath causing signs and symptoms of ischemia. Amputation of the digit was required despite repeated surgical debridement and decompression.

Conclusion
There have been previous reports of injection of oil-based vaccines into the human hand resulting in granulomatous inflammation or sterile abscess and causing morbidity and tissue loss.
Self-injection with veterinary vaccines is an occupational hazard for farmers and veterinary surgeons. Injection of vaccine into a closed compartment such as the human finger can have serious sequelae including loss of the injected digit. These injuries are not to be underestimated. Early debridement and irrigation of the injected area with decompression is likely to give the best outcome. Frequent review is necessary after the first procedure because repeat operations may be required.
Elevated levels of procoagulant microparticles in a patient with myocardial infarction, antiphospholipid antibodies and multifocal cardiac thrombosis
Authors: Morel O , Jesel L , Freyssinet JM , Toti F 
Published: October 2005

Abstract
Circulating procoagulant microparticles (MP) are pathogenic markers of enhanced coagulability associated to a variety of disorders and released from stimulated vascular cells. When derived from endothelial cells, MP were found characteristic of thrombotic propensity in primary antiphospholipid syndrome (APS). The prothrombotic status of a patient with antiphospholipid antibodies (APL), a past history of mesenteric vein thrombosis and presenting myocardial infarction and extensive intracardiac thrombosis was examined by measurement of circulating procoagulant MP. MP of platelet and endothelial origins were highly elevated with respect to values detectable in patients with myocardial infarction and no history of APS (6- and 3-fold elevation, respectively) or in healthy volunteers (13- and 25-fold elevation, respectively). In this particular patient, with moderate APL titer, a drastic release of procoagulant MP could have contributed to thrombus growth and the development of extensive intracardiac thrombosis.
The place of public inquiries in shaping New Zealand's national mental health policy 1858–1996
Authors: Brunton Warwick 
Published: October 2005

Abstract

Background
This paper discusses the role of public inquiries as an instrument of public policy-making in New Zealand, using mental health as a case study. The main part of the paper analyses the processes and outcomes of five general inquiries into the state of New Zealand's mental health services that were held between 1858 and 1996.

Results
The membership, form, style and processes used by public inquiries have all changed over time in line with constitutional and social trends. So has the extent of public participation. The records of five inquiries provide periodic snapshots of a system bedevilled by long-standing problems such as unacceptable standards, under-resourcing, and poor co-ordination. Demands for an investigation no less than the reports and recommendations of public inquiries have been the catalyst of some important policy changes, if not immediately, then by creating a climate of opinion that supported later change. Inquiries played a significant role in establishing lunatic asylums, in shaping the structure of mental health legislation, establishing and maintaining a national mental health bureaucracy within the machinery of government, and in paving the way for deinstitutionalisation. Ministers and their departmental advisers have mediated this contribution.

Conclusion
Public inquiries have helped shape New Zealand's mental health policy, both directly and indirectly, at different stages of evolution. In both its advisory and investigative forms, the public inquiry remains an important tool of public administration. The inquiry/cause and policy/effect relationship is not necessarily immediate but may facilitate changes in public opinion with corresponding policy outcomes long after any direct causal link could be determined. When considered from that long-term perspective, the five inquiries can be linked to several significant and long-term contributions to mental health policy in New Zealand.
The impact of HLA-DRB1genes on extra-articular disease manifestations in rheumatoid arthritis
Authors: Turesson Carl , Schaid Daniel J , Weyand Cornelia M , Jacobsson Lennart TH , Goronzy Jörg J , Petersson Ingemar F , Sturfelt Gunnar , Nyhäll-Wåhlin Britt-Marie , Truedsson Lennart , Dechant Sonja A , Matteson Eric L 
Published: October 2005

AbstractThe objective of this study was to examine HLA-DRB1 and HLA-DQB1 genotypes in patients with severe extra-articular rheumatoid arthritis (ExRA) and to compare them with the genotypes of rheumatoid arthritis (RA) patients without extra-articular manifestations. Patients with severe ExRA were recruited from a large research database of patients with RA, from two cohorts of prevalent RA cases, and from a regional multicenter early RA cohort. Cases with ExRA manifestations (n = 159) were classified according to predefined criteria. Controls (n = 178) with RA but no ExRA were selected from the same sources. Cases and controls were matched for duration of RA and for clinical center. PCR based HLA-DRB1 and HLA-DQB1 genotyping was performed using the Biotest SSP kit, with additional sequencing in order to distinguish DRB1*04 subtypes. Associations between alleles and disease phenotypes were tested using multiple simulations of random distributions of alleles. There was no difference in global distribution of HLA-DRB1 and HLA-DQB1 alleles between patients with ExRA and controls. DRB1*0401 (P = 0.003) and 0401/0401 homozygosity (P = 0.002) were more frequent in Felty's syndrome than in controls. The presence of two HLA-DRB1*04 alleles encoding the shared epitope (SE) was associated with ExRA (overall odds ratio 1.79, 95% confidence interval 1.04–3.08) and with rheumatoid vasculitis (odds ratio 2.44, 95% confidence interval 1.22–4.89). In this large sample of patients with ExRA, Felty's syndrome was the only manifestation that was clearly associated with HLA-DRB1*0401. Other ExRA manifestations were not associated with individual alleles but with DRB1*04 SE double dose genotypes. This confirms that SE genes contribute to RA disease severity and ExRA. Other genetic and environmental factors may have a more specific impact on individual ExRA manifestations.
E-cadherin endocytosis regulates the activity of Rap1: a traffic light GTPase at the crossroads between cadherin and integrin function
Authors: Balzac Fiorella , Avolio Maria , Degani Simona , Kaverina Irina , Torti Mauro , Silengo Lorenzo , Small J. Victor , Retta Saverio Francesco 
Published: October 2005

The coordinate modulation of cadherin and integrin functions plays an essential role in fundamental physiological and pathological processes, including morphogenesis and cancer. However, the molecular mechanisms underlying the functional crosstalk between cadherins and integrins are still elusive.Here, we demonstrate that the small GTPase Rap1, a crucial regulator of the inside-out activation of integrins, is a target for E-cadherin-mediated outside-in signaling. In particular, we show that a strong activation of Rap1 occurs upon adherens junction disassembly that is triggered by E-cadherin internalization and trafficking along the endocytic pathway. By contrast, Rap1 activity is not influenced by integrin outside-in signaling. Furthermore, we demonstrate that the E-cadherin endocytosis-dependent activation of Rap1 is associated with and controlled by an increased Src kinase activity, and is paralleled by the colocalization of Rap1 and E-cadherin at the perinuclear Rab11-positive recycling endosome compartment, and the association of Rap1 with a subset of E-cadherin-catenin complexes that does not contain p120ctn. Conversely, Rap1 activity is suppressed by the formation of E-cadherin-dependent cell-cell junctions as well as by agents that inhibit either Src activity or E-cadherin internalization and intracellular trafficking. Finally, we demonstrate that the E-cadherin endocytosis-dependent activation of Rap1 is associated with and is required for the formation of integrin-based focal adhesions.Our findings provide the first evidence of an E-cadherin-modulated endosomal signaling pathway involving Rap1, and suggest that cadherins may have a novel modulatory role in integrin adhesive functions by fine-tuning Rap1 activation.
Highly Efficient Dye-sensitized Solar Cells Based on Single Crystalline TiO2 Nanorod Film
Authors: Jiu Jinting , Wang Fumin , Isoda Seiji , Adachi Motonari 
Published: October 2005

Abstract
Single crystalline anatase TiO2 nanorods were successfully synthesized by surfactant-assisted hydrothermal method. A high light-to-electricity conversion yield of 7.06% was achieved by applying the TiO2 nanorods as thin film of dye-sensitized solar cells.
Rapid Formation of Novel Au Core–Ag Shell Nanostructures by a Microwave-polyol Method
Authors: Tsuji Masaharu , Miyamae Nobuhiro , Matsumoto Kisei , Hikino Sachie , Tsuji Takeshi 
Published: October 2005

Abstract
Au core–Ag shell bimetallic nanoparticles, denoted as Au@Ag, have been prepared by a microwave(MW)-polyol method through the two-step reduction of AuCl4− and Ag+ ions in the presence of poly(vinylpyrrolidone) (PVP) as a capping reagent. When single crystal polygonal Au nanoplates and nanorods were used as seeds, a mixture of various Au@Ag core–shell nanostructures was prepared after MW heating for only 2 min. Among them, small amounts of novel polygonal and rod types of Au@Ag nanostructures, in which morphologies of Au core were conserved, were produced.