bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three cases of extranodal NK/T‐cell lymphoma of the nasal type diagnosed by nasal brush cytology
Authors: Kishimoto Koji , Kitamura Takashi , Hirayama Yoshiko , Tate Genshu , Mitsuya Toshiyuki 
Published: January 2007

AbstractExtranodal natural killer (NK)/T‐cell lymphoma of the nasal type is a rare type of malignant lymphoma that is most common in Asian countries. Here we describe cytomorphologic, immunocytochemical, and molecular cytochemical features of three cases of NK/T‐cell lymphoma of the nasal type diagnosed by nasal brush cytology. Cytomorphologic findings common among the three cases included the presence of several cell types, including nasal cavity epithelial cells, histiocytes, phagocytic histiocytes, and lymphoid cells, within a necrotic background. Suspected lymphoma cells were medium to large lymphoid cells possessing light blue and abundant cytoplasm. A characteristic feature of these cells was the presence of the tongue‐like projections of cytoplasm from one or both sides of the cells. We believe these intriguing cytologic findings are indicators of NK/T‐cell lymphoma of the nasal type. Azurophilic granules were observed in all cases, ranging from extremely fine granules to large granular lymphocyte (LGL)‐like granules. Immunocytochemical and molecular cytochemical analyses showed staining for natural killer cell antigen CD56 as well as cytotoxic granule‐associated proteins granzyme B7 (GrB7) and T‐cell‐restricted intercellular antigen‐1 (TIA‐1). EBV (Epstein‐Barr virus) encoded small RNAs (EBER) positivity was shown by in situ hybridization, and no rearrangement of the TCRγ gene was observed. Comparison between cytobrush and cotton swab methodology showed that cytobrush resulted in more cell‐rich specimens than did cotton swabs, suggesting that nasal brush cytology with cytobrush is most useful in the diagnosis of NK/T‐cell lymphoma of the nasal type. Diagn. Cytopathol. 2007;35:125–129. © 2007 Wiley‐Liss, Inc.
Interaction of Bispyridinium Compounds with Phospholipid Layers in Presence and Absence of Electric Field
Authors: Merrifield Jonathan , Tattersall John , Bird Mike , Nelson Andrew 
Published: January 2007

AbstractThe nonspecific biological activity of compounds with cell membranes is of great importance when considering them for pharmaceutical applications. The interactions of a group of therapeutic bispyridinium compounds with phospholipid monolayers of dioleoyl phosphatidylcholine (DOPC) on a mercury electrode (HMDE) are reported in this paper. Impedance versus frequency plots and capacitance‐potential curves were recorded for the model membrane system. The penetration of the compounds into the monolayer is shown to be dependent on the applied electric field. Both electron withdrawing and electron donating substituents of the bispyridinium compounds promote interaction with the compounds at applied negative potential.
Binding of Cd2+ and Zn2+ with the Phytochelatin (γ‐Glu‐Cys)4‐Gly: A Voltammetric Study Assisted by Multivariate Curve Resolution and Electrospray Ionization Mass Spectrometry
Authors: Chekmeneva Elena , Díaz‐Cruz José Manuel , Ariño Cristina , Esteban Miquel 
Published: January 2007

AbstractThe complexation of Cd2+ and Zn2+ with the phytochelatin (γ‐Glu‐Cys)4‐Gly (PC4) is studied by Differential Pulse Polarography (DPP) assisted by Multivariate Curve Resolution with Alternating Least Squares (MCR‐ALS) and by Electrospray Ionization Mass Spectrometry (ESI‐MS). In the case of Zn2+, voltammetric results show the presence of an inert electroinactive Zn2+PC4 complex, which is confirmed by ESI‐MS. In the case of Cd2+, the unitary DPP peaks and the concentration profiles obtained by MCR‐ALS from DPP titrations of PC4 with Cd2+ suggest the formation of two electroactive Cd2+PC4 complexes. The first complex, with a 1 : 1 stoichiometry, shows a fixed DPP peak potential characteristic of a kinetically inert behavior. After further Cd2+ additions a second labile complex seems to be formed. This behavior is confirmed by ESI‐MS detection of CdPC4 and Cd2PC4 complexes.
Water quality in the River Clyde: a case study of additive and interaction models
Authors: McMullan A. , Bowman A. W. , Scott E. M. 
Published: January 2007

AbstractAdditive models are widely used to provide very flexible and effective descriptions of regression data where assumptions of linear relationships are too restrictive. However, these models usually assume independent errors and there are many applications where the data are correlated. This is often true in environmental settings and an application to water quality modelling in the River Clyde is described. Methods of fitting and analysing an additive model in this setting are discussed. An approach which fits an additive model in the usual way but incorporates the correlated error structure into the assessment of components is described. The methods are applied to the Clyde data to identify the factors which affect water quality, as well as spatial and temporal trends. Interaction terms, which allow the effects of explanatory variables to change smoothly with position down the river, are also explored. Particular emphasis is placed on approximateFtests to identify the most suitable models and the presence and nature of particular covariate effects. Copyright © 2007 John Wiley & Sons, Ltd.
Bayesian prediction of rainfall records using the generalized exponential distribution
Authors: Madi Mohamed T. , Raqab Mohammad Z. 
Published: January 2007

AbstractThe Los Angeles rainfall data are found to fit well to the two‐parameter generalized exponential (GE) distribution. A Bayesian parametric approach is described and used to predict the behavior of further rainfall records. Importance sampling is used to estimate the model parameters, and the Gibbs and Metropolis samplers are used to implement the prediction procedure. Copyright © 2007 John Wiley & Sons, Ltd.
A comparison of approaches to estimating confidence intervals for willingness to pay measures
Authors: Hole Arne Risa 
Published: January 2007

AbstractThis paper describes four approaches to estimating confidence intervals for willingness to pay measures: the delta, Fieller, Krinsky Robb and bootstrap methods. The accuracy of the various methods is compared using a number of simulated datasets. In the majority of the scenarios considered all four methods are found to be reasonably accurate as well as yielding similar results. The delta method is the most accurate when the data is well‐conditioned, while the bootstrap is more robust to noisy data and misspecification of the model. These conclusions are illustrated by empirical data from a study of willingness to pay for a reduction in waiting time for a general practitioner appointment in which all the methods produce fairly similar confidence intervals. Copyright © 2007 John Wiley & Sons, Ltd.
Informed consent in advanced laryngeal cancer
Authors: Foote Robert L. , Brown Paul D. , Garces Yolanda I. , Okuno Scott H. , Miller Robert C. , Strome Scott E. 
Published: January 2007

AbstractPurpose.This study was designed to evaluate the relationship between the informed consent process and the treatment received by patients with advanced laryngeal cancer.Methods.The study group was composed of 101 consecutive patients who were eligible for the Department of Veterans Affairs Laryngeal Cancer Study Group and Radiation Therapy Oncology Group/Head and Neck Intergroup (RTOG 91‐11) larynx preservation clinical trials and who underwent treatment at the Mayo Clinic during the years 1985 to 2000. Records were reviewed to evaluate the effect that referral to an oncologist, discussion and documentation of treatment alternatives by the surgeon, offering a chance at a larynx conservation surgical procedure, and treatment recommendation made by the surgeon have on the treatment chosen and received by the patient.Results.Discussion of treatment alternatives by the surgeon (p = .0054), referral to an oncologist (p < .0001), offering a chance for a larynx conservation surgical procedure (p = .047), and treatment recommended by the surgeon (p < .0001) were significantly related to the treatment received by the patient.Conclusion.The treatment received by a patient with advanced laryngeal cancer is determined by the treatment recommended by the surgeon, referral to an oncologist, discussion of treatment alternatives by the surgeon, and offering a chance for a larynx conservation surgical procedure. © 2007 Wiley Periodicals, Inc. Head Neck 29:, 2007.
Infrahyoid fascio‐myocutaneous flap as an alternative to free radial forearm flap in head and neck reconstruction
Authors: Deganello Alberto , Manciocco Valentina , Dolivet Gilles , Leemans C. René , Spriano Giuseppe 
Published: January 2007

AbstractBackground.The use of microvascular free flaps is currently the favored method for the reconstruction of defects after resection of head and neck cancer. The flap most commonly used for head and neck reconstruction is the free radial forearm flap, but the less popular infrahyoid flap represents a good alternative in selected cases. This flap has proven to be helpful in the reconstruction of a wide range of moderate‐sized head and neck defects.Methods.We reviewed a series of 13 patients with defects resulting from cancer of the head and neck, who underwent infrahyoid flap reconstruction as an alternative to free radial forearm flap. The series includes 12 squamous cell carcinomas arising from the oral cavity and oropharynx, and 1 Merkel cell carcinoma of the submental skin. In the harvesting of the flap, the technical modifications recently suggested by Dolivet et al were used in all cases. Furthermore, another technical change has been introduced so creating a new infrahyoid facio‐myocutaneous flap (IHFMCF).The surgical technique is described in detail.Results.No total or partial flap necrosis was experienced. All reconstructions healed quickly without wound complications and with good functional results. The healing process in the donor site was excellent in every case with good aesthetic results.Conclusions.The IHFMCF is a versatile, reliable, and convenient flap suitable for repairing small and medium‐sized defects of the oral cavity and oropharynx and obviates the need for a microvascular reconstruction. © 2007 Wiley Periodicals, Inc. Head Neck 29, 2007
Restoration of wild‐type p53 function in human cancer: Relevance for tumor therapy
Authors: Bossi Gianluca , Sacchi Ada 
Published: January 2007

AbstractBackground.In the majority of human cancers, the tumor suppressor activity of p53 is impaired because of mutational events or interactions with other proteins (ie, MDM2). The loss of p53 function is responsible for increased aggressiveness of cancers, while tumor chemoresistance and radioresistance are dependent upon the expression of mutant p53 proteins.Methods.Review of the literature indicates that p53 acts primarily as a transcription factor whose function is subject to a complex and diverse array of covalent post‐translational modifications that markedly influence the expression of p53 target genes responsible for cellular responses such as growth arrest, senescence, or apoptosis. The ability of p53 to induce apoptosis in cancer cells is believed essential for cancer therapy.Results.Numerous data indicate that p53 dependent apoptosis is a relevant factor in determining the efficacy of anticancer treatments. Thus, the development of new strategies for restoration of p53 function in human tumors is considered an important issue. Two main approaches for restoration of p53 function have been pursued that impact anticancer treatments: (a) de novo expression of wild‐type p53 (wt‐p53) through gene therapy and (b) identification of small molecules reactivating wt‐p53 function.Conclusions.The extensive body of knowledge acquired has identified manipulations of p53 signaling as a relevant issue for successful therapies. In this context, the recognition of p53 status in cancer cells is significant and would help considerably in the selection of an appropriate therapeutic approach. p53 manipulations for cancer therapy have revealed the need for specificity of p53 activation and ability to spare body tissues. Furthermore, the promising results obtained by using molecules competent to reactivate wt‐p53 functions in cancer cells provide the basis for the design of new molecules with lower side effects and higher anti‐tumor efficiency. The reexpression and reactivation of p53 protein in human cancer cells would increase tumor susceptibility to radiation or chemotherapy enhancing the efficacy of standard therapeutic protocols. © 2007 Wiley Periodicals, Inc. Head Neck 29, 2007
Multiple intraosseous hemangiomas—investigation and role of N‐butylcyanoacrylate in management
Authors: Syal Rajan , Tyagi Isha , Goyal Amit , Barai Sukanto , Parihar Anit 
Published: January 2007

AbstractBackground.Primary intraosseous hemangiomas are rare (0.7% of all osseous neoplasms), benign, slow‐growing neoplasms. These lesions are usually solitary. We are reporting a case of multicentric intraosseous hemangiomas. Investigation, treatment options, and role of N‐butylcyanoacrylate (NBCA) in management will be discussed.Methods and Results.A 20‐year‐old man had multicentric intraosseous hemangiomas involving the skull bones, mandible, vertebra, pelvic bone, and tibial tuberosity. N‐butylcyanoacrylate was used by direct puncture technique using a transosseous transcutaneous route to control profuse bleeding from the retromolar region.Conclusion.To the best of our knowledge, this is the first reported case with such extensive multicentric intraosseous hemangiomas. N‐butylcyanoacrylate by direct puncture technique can be an effective method to devascularize and stabilize low‐flow intraosseous vascular tumors. © 2007 Wiley Periodicals, Inc. Head Neck, 2007
Epithelioid osteosarcoma presenting as a rapidly expanding maxillary mass
Authors: Rinaggio Joseph , Kewitt Greg F. , McGuff H. Stan 
Published: January 2007

AbstractBackground.Osteosarcomas of the jaws account for less than 10% of reported neoplasms of this type. Patients with osteosarcoma in this region tend to be in the late‐third to mid‐fourth decades of life. The osteoblastic, chondroblastic, and fibroblastic variants constitute the majority of lesions.Methods.A mass was observed in the maxilla of a 50‐year‐old male who presented for a complete odontectomy. Over the ensuing weeks, the mass substantially increased in size, despite multimodality treatment efforts.Results.Radiographic findings revealed a mixed density mass of the left maxilla. Histologic, immunohistochemical, and electron microscopy examination of the tumor showed a malignant mesenchymal neoplasm consisting of sheets of epithelioid and spindle cells exhibiting focal osteoid formation.Conclusions.We report a rare and aggressive case of epithelioid osteosarcoma arising in the maxillofacial complex. © 2007 Wiley Periodicals, Inc. Head Neck, 2007
Twist, a novel oncogene, is upregulated in pancreatic cancer: Clinical implication of Twist expression in pancreatic juice
Authors: Ohuchida Kenoki , Mizumoto Kazuhiro , Ohhashi Seiji , Yamaguchi Hiroshi , Konomi Hiroyuki , Nagai Eishi , Yamaguchi Koji , Tsuneyoshi Masazumi , Tanaka Masao 
Published: January 2007

AbstractDespite evidence that Twist, a highly conserved basic helix‐loop‐helix transcription factor, is a novel oncogene, there are no reports describing Twist expression in pancreatic cancer. Intraductal papillary mucinous neoplasm (IPMN) and pancreatic intraepithelial neoplasia (PanIN) are precursor lesions of pancreatic cancer. To clarify involvement of Twist expression in pancreatic cancer, we used quantitative reverse transcription‐polymerase chain reaction and examined Twist expression in pancreatic cancer, IPMN, and non‐neoplastic pancreas using bulk tissues (11 cancers, 18 IPMNs, and 15 non‐neoplastic pancreata), microdissected cells (cancer from 22 sections, IPMN from 19 sections, PanIN from 6 sections, and pancreatitis‐affected epithelial cells from 14 sections), and pancreatic juice (16 from cancer, 28 from IPMN, and 17 from pancreatitis). Twist expression differed significantly between cancer and IPMN bulk tissues (p < 0.0001) but not between cancer and non‐neoplastic tissues. Twist expressions differed significantly between microdissected cancer cells, IPMN cells, and pancreatitis‐affected cells (all comparisons, p < 0.017). PanIN cells expressed significantly lower levels of Twist than did IDC cells (p = 0.016). Twist expression differed significantly between cancer and IPMN juice samples (p = 0.0002) but not between cancer and pancreatitis juice samples. Receiver operation characteristic curve analyses revealed that measurement of Twist was more useful for discriminating cancer from IPMN than from chronic pancreatitis (p = 0.009). Our results suggest that Twist is involved in tumor progression of pancreatic cancer and that measurement of Twist in pancreatic juice may be useful to differentiate pancreatic cancer from nonmalignant neoplasms such as IPMN. © 2007 Wiley‐Liss, Inc.
Combined analysis of HPV‐DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer
Authors: Reimers Niklas , Kasper Hans U. , Weissenborn Soenke J. , Stützer Hartmut , Preuss Simon F. , Hoffmann Thomas K. , Speel Ernst Jan M. , Dienes Hans P. , Pfister Herbert J. , Guntinas‐Lichius Orlando , Klussmann Jens P. 
Published: January 2007

AbstractMolecular prognostic indicators for oropharyngeal squamous cell carcinoma (OSCC), including HPV‐DNA detection, epidermal growth factor receptor (EGFR) and p16 expression, have been suggested in the literature, but none of these are currently used in clinical practice. To compare these predictors, 106 newly diagnosed OSCC for the presence of HPV‐DNA and expression of p16 and EGFR were analyzed. The 5‐year disease‐free survival (DFS) and overall survival (OS) were calculated in relation to these markers and a multivariate Cox analysis was performed. Twenty‐eight percent of the cases contained oncogenic HPV‐DNA and 30% were positive for p16. The p16 expression was highly correlated with the presence of HPV‐DNA (p < 0.001). Univariate analysis of the 5‐year DFS revealed a significantly better outcome for patients with p16‐positive tumors (84% vs. 49%, p = 0.009). EGFR‐negative tumors showed a tendency toward a better prognosis in DFS (74% vs. 47%, p = 0.084) and OS (70% vs. 45%, p = 0.100). Remarkable and highly significant was the combination of p16 and EGFR expression status, leading to 5‐year DFS of 93% for p16+/EGFR− tumors vs. 39% for p16−/EGFR+ tumors (p = 0.003) and to a 5‐year OS of 79% vs. 38%, respectively (p = 0.010). In multivariate analysis p16 remained a highly significant prognostic marker for DFS (p = 0.030) showing a 7.5‐fold increased risk for relapse in patients with p16‐negative tumors. Our data indicate that p16 expression is the most reliable prognostic marker for OSCC and further might be a surrogate marker for HPV‐positive OSCC. HPV+/p16+ tumors tended to have decreased EGFR expression, but using both immunohistological markers has significant prognostic implications. © 2007 Wiley‐Liss, Inc.
Expression of centrosome‐associated gene products is linked to tetraploidization in mantle cell lymphoma
Authors: Neben Kai , Ott German , Schweizer Silja , Kalla Jörg , Tews Björn , Katzenberger Tiemo , Hahn Meinhard , Rosenwald Andreas , Ho Anthony D. , Müller‐Hermelink Hans Konrad , Lichter Peter , Krämer Alwin 
Published: January 2007

AbstractIn mantle cell lymphoma (MCL), a blastoid variant with a striking tendency to harbor chromosome numbers in the tetraploid range has been identified. Centrosome aberrations have recently been implicated in the induction of aneuploidy in many human malignancies including MCL by malsegregation of chromosomes during anaphase of mitosis. Recently, we showed that centrosome aberrations occur more frequently in tetraploid MCL as compared to their diploid counterparts. To test the hypothesis of an association between tetraploidization and expression of genes coding for centrosomal proteins in MCL, tumor RNA of 33 MCL samples was hybridized to custom‐made cDNA microarrays, representing 4,628 distinct human gene‐specific fragments, with particular enrichment for cancer‐relevant (n = 2,440) and centrosome‐associated genes (n = 359). Notably, 4 of the 6 most significant genes (CAMKK2, PCNT2, TUBGCP3, TUBGCP4) discriminating between diploid and near‐tetraploid MCL code for centrosomal proteins. As confirmed by quantitative RT‐PCR analysis, calcium/calmodulin‐dependent protein kinase II (CAMKK2), pericentrin (PCNT2) and γ‐tubulin complex associated protein 3 (TUBGCP3) were all found to be significantly higher expressed in near‐tetraploid than in diploid MCL samples. In conclusion, we describe a comprehensive expression signature of a set of genes associated with tetraploidization in MCL. The high expression level of centrosome‐associated gene products in blastoid MCL matches the description of more frequent centrosome aberrations in this MCL variant. © 2007 Wiley‐Liss, Inc.
Management of early breast cancer in the elderly
Authors: Rao Vittal S.R. , Garimella Veerabhadra , Hwang Meiju , Drew Philip J. 
Published: January 2007

AbstractBreast cancer is the most common malignancy in women with an age related increase in incidence ranging from 1 in 50 at age 50 to 1 in 10 at age 80. This is particularly significant in view of the changing demographics in the western population, characterised by an aging population and increased life expectancy. However in spite of favourable prognostic factors and less aggressive biological behaviour, elderly breast cancer patients receive less aggressive treatment when compared with their younger counterparts. Appropriate treatment should be offered depending on physiological reserve and comorbidities. Primary endocrine treatment has been shown to be associated with significant morbidity in terms of disease progression. Prompt surgery and adjuvant treatment can decrease relapse and improve survival. Radiation therapy is shown to decrease local relapse and chemotherapy may have a role in a select group of patients with adverse prognostic factors. With incidence of breast cancer bound to increase in the elderly population, it is essential to establish optimum therapy in this cohort of patients as studies reveal good outcome from standard treatment. © 2007 Wiley‐Liss, Inc.
Biomimetic interfacial interpenetrating polymer networks control neural stem cell behavior
Authors: Saha Krishanu , Irwin Elizabeth F. , Kozhukh Julia , Schaffer David V. , Healy Kevin E. 
Published: January 2007

AbstractHighly‐regulated signals surrounding stem cells, such as growth factors at specific concentrations and matrix mechanical stiffness, have been implicated in modulating stem cell proliferation and maturation. However, tight control of proliferation and lineage commitment signals is rarely achieved during growth outside the body, since the spectrum of biochemical and mechanical signals that govern stem cell renewal and maturation are not fully understood. Therefore, stem cell control can potentially be enhanced through the development of material platforms that more precisely orchestrate signal presentation to stem cells. Using a biomimetic interfacial interpenetrating polymer network (IPN), we define a robust synthetic and highly‐defined platform for the culture of adult neural stem cells. IPNs modified with two cell‐binding ligands, CGGNGEPRGDTYRAY from bone sialoprotein [bsp‐RGD(15)] and CSRARKQAASIKVAVSADR from laminin [lam‐IKVAV(19)], were assayed for their ability to regulate self‐renewal and differentiation in a dose‐dependent manner. IPNs with >5.3 pmol/cm2 bsp‐RGD(15) supported both self‐renewal and differentiation, whereas IPNs with lam‐IKVAV(19) failed to support stem cell adhesion and did not influence differentiation. The IPN platform is highly tunable to probe stem cell signal transduction mechanisms and to control stem cell behavior invitro. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007