First Report on Pathogenic Variability of Different Isolates of Diaporthe phaseolorum var. meridionalis on Soybean in Argentina
Authors: Pioli R. N. , Morandi E. N. , Gosparini C. O. , Borghi A. L. 
Published: May 2007

The objective of this study was to characterize the pathogenicity of several local isolates of Diaporthe phaseolorum (Cooke & Ellis) Sacc. var. meridionalis Fernández and its anamorph, Phomopsis phaseoli (Desmaz.) Sacc. meridionalis Morgan-Jones, the causal agent of southern stem canker of soybean (Glycine max (L.) Merr.), in soybean lines carrying major resistance genes. Soybean plants with typical stem canker symptoms were collected during the 1996 to 1997 and 1997 to 1998 growing seasons in the central and southern areas of Santa Fe Province, Argentina. The pathogen was isolated from the internal tissues of infected stems, cultured on potato glucose agar acidified with 0.2% lactic acid (APGA), amended with streptomycin at 100 mg/liter, and maintained in the dark at 25 ± 1°C. Isolates were characterized based on the morphology of colonies, perithecia, and pycnidia and measurement of asci, bicellular, biguttulate ascospores, and alpha conidia (1). Soybean cultivars used to assay pathogenicity included Tracy M (Rdc1 and Rdc2 genes), Isoline I (Tracy Misoline with only the Rdc1 gene), Isoline II (Tracy M isoline with only the Rdc2 gene), Crockett (Rdc3 gene), Hutchinson (Rdc4 gene), and RA 702 (susceptible cultivar). Hypocotyls of 14-day-old seedlings grown in the greenhouse were inoculated by the toothpick method. Four replicates of nine seedlings each were used. Seedlings punctured with sterile toothpicks served as controls. The experiment was repeated twice with similar results. The D. phaseolorum var. meridionalis isolates assayed and their collection locations were Dpm1 (Malabrigo), Dpm2 (Los Molinos), Dpm3 (San Justo), Dpm5 (Oliveros), Dpm6 (San Jerónimo), and Dpm7 (Clarke). Twenty-eight days after inoculation, stem canker reactions were measured as the percentage of dead plants. The pathogen was reisolated from stems of randomly chosen symptomatic plants on day 14 after inoculation. These plants were included in the calculation of the percentage of dead plants. In control plants, lesions were not detected, and mycelial growth did not occur from stem portions plated on APGA. Tracy M and RA 702 had 0 to 7% dead plants and 70 to 95% dead plants, respectively, with all assayed isolates. Cultivars with single resistance genes reacted differently to various isolates. Isolates Dpm1 and Dpm3 caused little or no stem canker (<10% dead plants) on all cultivars with resistance genes. Isolates Dpm2 and Dpm6 killed 56 and 52%, respectively, of Isoline II (Rdc2 gene) plants. Isolates Dpm2 and Dpm7 killed 25% of cv. Hutchinson (Rdc4 gene) and Isoline I (Rdc1 gene) plants, respectively. Isolate Dpm5 killed <12% of plants with genes Rdc1, Rdc2, or Rdc3. The reaction of isolate Dpm5 with Hutchinson (Rdc4 gene) was not evaluated. The pathogenic diversity of these isolates of D. phaseolorum var. meridionalis may have been induced by the wide diffusion of resistant host cultivars (2). References: (1) F. A. Fernández and R. T. Hanlin. Mycologia 88:425, 1996. (2) A. W. Zhang et al. Phytopathology 88:1306, 1998.