bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isoniazid tumorigenicity in mice under different experimental conditions
Authors: Bhide S. V. , Maru G. B. , Sawai M. M. , Ranadive K. J. 
Published: February 2007

AbstractTumorigenicity of isoniazid in Swiss and A strain mice was studied with three different doses (0.55, 1.1 and 2.2 mg/mouse/day). Mice were fed isoniazid by gastric intubation and killed when they appeared weak. Incidence of tumour ranged from 32 to 100%. Subsequently sex‐specificity in isoniazid tumorigenicity was also studied as well as the effect of vitamin B complex or protein deficiency, and of transplacental exposure of Swiss strain embryos to isoniazid. It was observed that protein deficiency and transplacental exposure stimulated tumour incidence in mice treated with 0.55 mg of isoniazid. No sex specificity was observed in isoniazid tumorigenesis.
Polyadenylated RNA molecules and polyribosomes in tumours of chemical and viral origin
Authors: Shaposhnikov Jacob D. , Ratovitski Edward A. 
Published: February 2007

AbstractThe distribution of poly(A)‐enriched mRNA in nuclei, mitochondria, free and membrane‐bound polyribosomes from normal C3HA mouse and Syrian hamster livers and normal chicken fibroblasts has been compared with that in corresponding subcellular fractions in a transplantable, chemically induced MD hepatoma, non‐virus‐producer hamster and virus‐producer chicken Rous sarcomas. It has been shown that the content of poly(A)‐RNA is increased in all tumour fractions except in free polyribosomes. The distribution of different classes of polysomes i.e. free, membrane‐bound and mitochondrial outer‐membrane‐associated polysomes in tumour cells was changed in comparison to that in normal cells. It is concluded that in tumours of chemical and viral origin, the observed changes in the two components of the protein‐synthesizing apparatus occur simultaneously.
Comparison between the macrophage electrophoretic mobility (mem) and the fixed tanned erythrocyte electrophoretic mobility (fteem) tests in the detection of cancer
Authors: Harlos J. P. , Weiss L. 
Published: February 2007

AbstractWhen peripheral lymphocytes from patients with a history of cancer are incubated with encephalitogenic factor (EF), in 90% of cases the resulting products reduce the net surface negativity of guinea‐pig macrophages, used as detector cells, as revealed in the macrophage electrophoretic mobility (MEM) test. The MEM test is positive in 36% of people with no history of cancer. Formaldehyde‐fixed tanned sheep erythrocytes have been used as detector cells in place of guinea‐pig macrophages, in a fixed tanned erythrocyte electrophoretic mobility (FTEEM) test, with lymphocyte products identical to those used in MEM tests. In patients with a history of cancer, positive results were obtained in 28/42 cases with the FTEEM test compared with 32/42 in the MEM test. In people with no history of cancer, negative results were obtained in 16/18 cases with the FTEEM test, compared with 12/18 in the MEM test in the present series, and 51/69 in a more extensive series. These differences are not significant. Cases in which discrepancies are revealed between the two tests are discussed in terms of individual case histories.
Early increase of serum alpha‐fetoprotein in spontaneous hepatocarcinogenesis in mice
Authors: Jalanko H. , Virtanen I. , Engvall E. , Ruoslahti E. 
Published: February 2007

AbstractThe concentration of serum alpha‐fetoprotein (AFP) was followed in C3H mice having a high incidence of spontaneous liver‐cell cancer. No general elevation of serum AFP level with age was seen in mice without tumor. With a single exception, mice bearing hepatocellular carcinomas had increased serum AFP levels. In some mice this increase followed a biphasic course. Mice killed within 1 month of the time when an elevation of serum AFP was first observed had small tumors or no detectable tumor. Premalignant lesions were present in the livers of 11 out of 16 mice that had elevated AFP but no cancer, while only one out of 14 mice with normal AFP had such alterations. Our results strongly suggest that spontaneous hepatocarcinogenesis proceeds through almost the same premalignant lesions as chemically induced carcinogenesis, and that an increase in AFP production occurs early during this process, often preceding macroscopic lesions. Autologous antibodies to AFP were produced in a group of C3H mice by immunization with rat AFP. These anti‐AFP antibodies reduced the amount of serum AFP but had no effect on the incidence of spontaneous hepatomas.
Mouse natural killer (NK) cell activity against human cell lines is not influenced by superinfection of the target cell with xenotropic murine c‐type virus
Authors: Kiessling Rolf , Fenyö Eva Maria , Steinitz Michael , Klein George , Haller Otto 
Published: February 2007

AbstractMouse natural killer (NK) cells can lyse a variety of syngeneic, allogeneic and xenogeneic target cells in short‐term 51Cr release assays. The target specificity of NK cells is not known, but endogenous C‐type viral antigens have been suggested as possible target structures. To test this hypothesis, human lymphoid lines were superinfected with xenotropic mouse C‐type virus either by repeated passage through nude mice or by in vitro superinfection with the supernatants of nude‐mouse‐passaged lines. The appearance of surface‐associated MuLV antigens after superinfection was confirmed in a complement‐dependent cytotoxtcity test. Subsequently, the NK sensitivity of each infected line was compared with its non‐infected counterpart in direct cytolytic and competition assays, None of these two assay systems showed a consistent difference in NK sensitivity of infected and non‐infected cell lines. These findings do not lend support to the concept that murine C‐type viral antigens are responsible for NK sensitivity.
Effectiveness of clinically active antineoplastic drugs in a surgical‐adjuvant chemotherapy treatment regimen using the lewis lung (LL) carcinoma
Authors: Merker Philip C. , Wodinsky Isidore , Cantor Margaret L. , Venditti John M. 
Published: February 2007

AbstractSurgical‐adjuvant chemotherapy studies were done with BDF1 mice bearing Lewis Lung (LL) carcinoma implanted intramuscularly (IM). Ten days (D10) after implantation, the tumor‐bearing leg was surgically amputated, and intraperitoneal (IP) drug treatment was started either 3 or 5 days after surgery using single‐dose therapy or intermittent treatment given every 4 days for three doses or daily treatment for five doses. The percentage increase in life span (%ILS) was used as the primary measure for evaluation. Data for 17 clinically active drugs showed that surgical‐adjuvant, single‐agent chemotherapy was markedly effective (%ILS>100) in prolonging the life span of tumor‐bearing animals given BCNU, MeCCNU, and cytoxan; moderate activity (%ILS, 40–100) was obtained with bleomycin and vincristine, and marginal activity (%ILS, 35–40) was observed with 5‐fluorouracil (5‐FU), hexamethylmelamine (HMM), procarbazine, and dibromodulcitol. Surgical‐adjuvant, two‐drug‐combination chemotherapy treatment with bleomycin plus MeCCNU or cis‐platinum [cis‐PT(II)] plus CCNU were more effective than single‐drug chemotherapy in prolonging the life span of tumor‐bearing animals. When used in a surgical‐adjuvant chemotherapy‐treatment schedule, the solid tumor model was found to be sensitive to certain antineoplastic drugs that are of clinical benefit in the treatment of human lung cancer. Thus, the model is potentially useful in the search for newer single agents and combinations of drugs against cancers.
Neoplastic transformation of hamster brain cells in vitro by polyoma virus
Authors: De Micco C. , Tripier M. F. , Hassoun J. , Lipcey C. , Meyer G. , Toga M. 
Published: February 2007

AbstractThe transformation of cultivated hamster brain cells by polyoma virus is reported. The transformed cell line contained polyoma virus‐specific nuclear, surface and transplantation antigens. Subcutaneous and intracranial inoculations revealed high tumorigenicity of the cells. Brain‐specific S 100 protein was found in these tumors with immuno‐peroxidase staining, suggesting that they were of a nervous nature. Both in vivo and in vitro, the cells had glial features as studied by phase contrast, light and electron microscopy. Type‐H virus‐like particles were found in the tumor cells and might have played a role in the viral transformation.
Altered membrane‐associated functions in chronic lymphocytic leukemia cells
Authors: Godal Tore , Henriksen Aase , Ølandaas Tone , Iversen Jens‐Gustav , Lindmo Tore 
Published: February 2007

AbstractPeripheral blood lymphocytes consisting mainly of neoplastic B cells from patients with chronic lymphocytic leukemia (CLL cells) showed a markedly reduced response to the human B‐cell mitogens anti‐β2 microglobulin, Sepharose‐bound protein A and Sepharose‐bound anti‐human immunoglobulin (anti F(ab′)2) in all of nine patients studied. On the other hand, CLL cells from three out of eight patients tested responded well to the calcium ionophore A23187. Sepharose‐bound protein A and anti‐β2 microglobulin also failed to induce increased uptake of 86Rubidium (potassium analogue) in CLL cells as compared to B‐cell‐enriched preparations of normal peripheral blood lymphocytes. The capacity of CLL cells to cap various surface markers including β2 microglobulin was reduced. On the other hand, surface concentrations of β2 microglobulin were not reduced as measured by fluorescein‐labelled anti‐β2‐microglobulin in singlecell cytofluorometry. It is concluded that various membrane‐associated events elicited by ligandreceptor interactions are altered or blocked in CLL cells.
Analysis of serum blocking factors against leukocyte‐dependent antibody in melanoma patients
Authors: Murray E. , Ruygrok S. , Hersey P. , Milton G. W. 
Published: February 2007

AbstractPrevious studies have shown that leukocyte‐dependent antibody activity against melanoma cells in the sera of melanoma patients is often completely blocked by factors in their sera. In the present study, characterization of these factors blocking melanoma LDA was carried out by a number of sequential separation procedures involving gel filtration on Sepharose 6B, affinity chromatography on concanavalin A (Con A) and electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulphate (PAGE‐SDS). The blocking factors were identified throughout the separation procedure by their blocking activity against melanoma LDA. It was found that blocking activity against melanoma LDA was located in small (<60,000) and large (approx. 300,000) molecular weight fractions obtained by gel filtration. Both of the latter fractions were found to have affinity for concanavalin A and were therefore glucose‐ and/or mannose‐containing glycoproteins. The blocking activity in both fractions was further resolved into a single fraction of 15,000 d by PAGE‐SDS. This small molecular weight fraction appeared to be melanoma antigen as shown by its removal by affinity chromatography on melanoma antibody bound to sepharose and by its blocking activity against melanoma LDA. A similar fraction was isolated by this separation procedure from supernatants and membranes of cultured melanoma cells. These results appear to provide a basis for the development of in vitro assays to monitor tumour growth in melanoma patients and for further characterization of the biological nature of melanoma antigens.
In vitro generation of cytotoxicity against autologous human tumour biopsy cells
Authors: Vose B. M. , Vánky F. , Klein Eva , Fopp M. 
Published: February 2007

AbstractBlood lymphocytes from 22 cancer patients were examined for cytotoxicity against autologous tumour cells in a short‐term 51Cr release assay. Only three showed reactivity. In an attempt to increase cytotoxic potential in these and induce reactivity in non‐reactive cases, the lymphocytes were cultured alone or with autologous tumour cells for 6 days. Upon subsequent testing against frozen, stored targets, nine samples reacted, including two of those with primary reactivity. In seven cases augmented cytotoxicity was evident in mixed cultures compared with lymphocytes cultured alone. Two of 10 cases showed cytotoxicity against the K562 cell line after culture and, in two of 13 tests in which allogeneic tumour biopsy targets were used, weak reactivity was bound. Cytotoxicity for autologous tumour biopsy cells was uniformly accompanied by positive blastogenesis in MLTI assays. In four cases blastogenesis occurred without the induction of cytotoxicity.
Proteolytic release of antigenic fragments corresponding to normal fecal antigen and non‐specific cross‐reacting antigen from carcinoembryonic antigen
Authors: Matsuoka Y. , Koga Y. , Maruta H. , Yoshino M. , Tsuru E. 
Published: February 2007

AbstractThree immunogenic parts have so far been identified in the carcinoembryonic antigen (CEA) molecule. These are: determinants cross‐reacting with the normal fecal antigen (NFA) (NFA determinant); determinants cross‐reacting with the non‐specific cross‐reacting antigen (NCA) (NCA determinant); and determinants which appear to be more cancer‐specific (cancer determinant). The chemical nature of these parts of the CEA molecule was investigated by digestion with proteolytic enzymes together with anti‐CEA preparations with which these three immunogenic parts of CEA molecule could be identified. The CEA digest obtained with pepsin did not react in immunodiffusion and radioimmunoassay, indicating that pepsin completely destroyed all the antigenic parts. Digestion by pronase E destroyed only the cancer determinant and liberated two antigenic fragments corresponding to the NFA determinant and the NCA determinant, respectively. These results suggest that the cancer determinant may reside in a protein or a peptide part of the molecule. The chemical nature of the NFA and NCA determinant remains to be clarified.
Induction of mammary tumors in C57BL/haag female mice by a low oncogenic mammary tumor virus
Authors: Vlahakis G. , Chopra H. C. , Sawdon M. R. 
Published: February 2007

AbstractMammary tumor virus (MuMTV)‐negative mouse substrain C57BL/Haag foster‐nursed by strain DD becomes a line with a moderately high incidence of mammary tumors occurring in the females at a late age. Electron microscopy revealed B particles in the tumors, providing additional evidence for the propagation and transmission of DD‐MuMTV in C57BL/HaagfDD. Since both albino strains DD and RIII originated in Europe, develop pregnancy‐dependent mammary tumors, carry black and agouti genes (cc, BB, AA), and therefore may be related, evidence is presented which suggests that the two naturally occurring MuMTVs from DD and RIII may also be related, and that they may share a common origin.
Transformation of marmoset lymphocytes in vitro with herpesvirus ateles
Authors: Falk Lawrence , Johnson Donald , Deinhardt Friedrich 
Published: February 2007

AbstractCirculating lymphocytes from three species of marmoset monkeys were transformed in vitro with Herpesvirus ateles (HVA) either by co‐cultivation with lethally X‐irradiated HVA‐producing lymphoblastoid cells or by infection with cell‐free virus: 42 transformed lymphocyte cultures were obtained from 72 transformation attempts. Attempts to transform squirrel monkey lymphocytes were unsuccessful in 29 attempts. Association of HVA with each transformed culture was demonstrated by staining of antigen‐positive cells in indirect fluorescent antibody (FA) tests and recovery of HVA after co‐cultivation of the transformed cells with permissive monolayer cells. Cells of most transformed cultures possessed T lymphocyte properties: i.e. formation of E rosettes, reactivity with a specific anti‐marmoset T lymphocyte serum and lack of surface Ig.
Lysis of RSV‐transformed japanese quail cells by a factor from normal quail serum
Authors: Yoshikawa Yasuhiro , Yamanouchi Kazuya , Hishiyama Michiko , Kobune Kaoru 
Published: February 2007

AbstractNormal sera of Japanese quails caused lysis of tumor cells from a Rous sarcoma virus (RSV)‐induced quail tumor (QT cells). Other tumor cell lines, including RSV‐transformed quail embryo cells and methylcholanthrene‐induced quail tumors, were not lysed. This naturally occurring cytolytic factor (NCLF) was sensitive to heating at 56° C, zymosan, and inulin, and it required magnesium but not calcium for the expression of its activity. These results suggested that NCLF activity was mediated by complement activated through an alternative pathway. This possible complement activation occurred in the absence of specific antibodies to the target cells.
Abnormalities of chromosome no. 1 in human solid malignant tumours
Authors: Kovacs G. Y. 
Published: February 2007

AbstractMarker chromosomes involving chromosome No. 1 were studied with banding techniques in 10 primary solid tumours. Structural or numerical aberrations of chromosome No. 1 were found in nine of these. Two major types of rearrangement of chromosome No. 1 were observed: translocation of the long arm (partial trisomy) and an isochromosome of 1q. Earlier chromosome studies and the present cases suggest that regions near the centromere and q21 are vulnerable points on human chromosome No. 1, and that region q21–32 is important for development of certain tumour types.
Search for infective mammalian type‐C virus‐related genes in the dna of human sarcomas and leukemias
Authors: Nicolson M. O. , McAllister R. M. , Gilden R. V. , Charman H. , Rice N. , Heberling R. 
Published: February 2007

AbstractDNA was extracted from two human sarcoma cell lines, TE‐32 and TE‐418, and the leukemic cells from five children with acute myelocytic leukemia, three children with acute lymphocytic leukemia and four adults with acute myelocytic leukemia. The DNAs, assayed for infectivity by transfection techniques, induced no measurable virus by methods which would detect known mammalian C‐type antigens or RNA‐directed DNA polymerase in TE‐32, D‐17 dog cells and other indicator cells, nor did they recombine with or rescue endogenous human or exogenous murine or baboon type‐C virus. Model systems used as controls were human sarcoma cells, TE‐32 and HT‐1080, and human lymphoma cells, TE‐543, experimentally infected with KiMuLV, GaLV or baboon type‐C virus, all of which released infectious virus and whose DNAs were infectious for TE‐32 and D‐17 dog cells. Other model systems included. two baboon placentas and one embryonic cell strain spontaneously releasing infectious endogenous baboon virus and yielding DNAs infectious for D‐17 dog cells but not for TE‐32 cells. Four other baboon embryonic tissues and two embryonic cell strains, releasing either low levels of virus or no virus, did not yield infectious DNA.
Somatic cell hybrids between human lymphoma lines. IV. Establishment and characterization of a p3HR‐1/daudi hybrid
Authors: Ber R. , Klein G. , Moar M. , Rosén A. , Westman A. , Yefenof E. , Povey S. , Zeuthen J. 
Published: February 2007

AbstractA new human B‐lymphoma hybrid line designated DIP‐1 was derived from the fusion of the Burkitt lines Daudi and P3HR‐1. Cytogenetic analyses proved hybridity and showed that the hybrid was nearly complete from the chromosomal point of view. Hybridity was also confirmed by isozyme marker tests. The Daudi‐derived IgM‐kappa ring was fully exposed on DIP‐1, together with the P3HR‐1‐derived human beta‐2‐microglobulin. Expression of C3 receptors was intermediate between the two parents and EBV receptors were some what reduced in comparison with both. The spontaneous low‐level EBV antigen (EA and VCA) production of the parents was maintained and amplified in the hybrid. The hybrid, like its Daudi parent, was highly inducible with IdUrd. In P3HR‐1 virus super‐infection experiments, the Daudi parent was more permissive than the BU‐P3HR‐1 parent. The hybrid was intermediate, resembling the previously studied Raji/Daudi hybrid and contrasting with Raji/Namalwa and Raji/BJAB hybrids. Virus DNA replication patterns after P3HR‐1 virus superinfection resembled the antigen pattern. The implication of these findings for the understanding of virus‐host cell relationships and the regulation of the viral cycle is discussed The findings are meant to imply that genetically determined isozyme markers are autonomously expressed, as in other systems. Differentiation‐related markers and EBV‐cycle‐related characteristics are autonomously expressed in some cases (surface immunoglobulin, beta‐2‐microglobulin, spontaneous EBV‐production, IdUrd inducibility) and appear to be under a restrictive control in others (EA inducibility by P3HR‐1 virus superinfection, C3 and EBV receptors, viral DNA replication).
Chromosome 14q+ in a retinoblastoma
Authors: Hossfeld D. K. 
Published: February 2007

AbstractA chromosome 14q+ was found in the tumor cells of a patient with sporadic, bilateral retino‐blastoma. Eighty‐six per cent of the metaphases contained the marker. Some metaphases showed a chromosome 6q— in addition. The question of the specificity of such markers in lymphoid malignancies is raised.
Activation of endogenous type‐C viral p30 antigen in chemically‐induced rat hepatocellular carcinomas
Authors: Becker Frederick F. , Sherr Charles J. 
Published: February 2007

AbstractChemically induced rat hepatocellular carcinomas were prospectively examined for expression of the major group‐specific antigen (p30) of genetically transmitted rat type‐C viruses. Ten primary tumors induced following oral administration of the carcinogen N‐2‐acetylaminofluorine did not express elevated levels of viral antigen as compared to antigen levels detected in normal liver tissue. By contrast, rapidly growing transplantable hepatocellular carcinomas (THCs) derived from primary tumors expressed increased quantities of viral antigen. The expression of antigen was marginally elevated after only one transplantation, increased to maximal levels after several transplant generations and, once achieved, was stably maintained throughout subsequent transplants. Studies with additional previously established THCs showed that poorly differentiated, rapidly proliferating tumors tended to express elevated levels of viral antigen, while more differentiated, slowly growing tumors did not. The results show that the expression of endogenous type‐C viral 30 antigen is a stable phenotypic property of many rat THC lines.
Rapidly progressing breast cancer (poussée Évolutive) in Tunisia: Studies on delayed hypersensitivity
Authors: Mourali Nejib , Levine Paul H. , Tabanne Francoise , Belhassen Slim , Bahi Jacqueline , Bennaceur Meri , Herberman Ronald B. 
Published: February 2007

AbstractDelayed hypersensitivity reactions to a battery of antigens were measured in 145 Tunisian breast cancer patients to determine whether an immuno‐logic mechanism could be detected which might explain the high frequency (60%) of the rapidly progressing form in Tunisian breast cancer patients. Although a greater proportion (30%) of patients with rapidly progressing breast cancer reacted to extracts of a breast tumor antigen (2937) than patients without PEV (9%), no significant difference between PEV and non‐PEV patients could be found in reactivity to DNCB, standard microbial antigens, or extracts from tissue culture cell lines. Rapidly progressing breast cancer in Tunisia is not associated with an impairment of delayed hypersensitivity.