bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of GH gene polymorphism and sex on carcass traits and fatty acid compositions in Japanese Black cattle
Authors: ARDIYANTI Astrid , OKI Yoshie , SUDA Yoshihito , SUZUKI Keiichi , CHIKUNI Koichi , OBARA Yoshiaki , KATOH Kazuo 
Published: October 2008

ABSTRACTTo investigate the effects of bovine growth hormone (bGH) gene polymorphism on carcass traits and fatty acid compositions in Japanese Black cattle caused by nucleotide substitution of CTG (allele A)/GTG (allele B) at codon 127 and of ACG (allele A and B)/ATG (allele C) at codon 172 of bGH, GH genotypes of 135 cattle were determined using allele specific‐multiplex polymerase chain reaction (PCR). Allele A gave greater rib thickness and lower melting point of fat (MP) while allele B gave higher C18:1% (P < 0.05). Allele C gave higher C18:1, monounsaturated fatty acid (MUFA), unsaturated fatty acid (USFA) percentages (P < 0.05). It also gave lower saturated fatty acid (SFA) percentages, higher MUFA/SFA and USFA/SFA ratios, and lower MP (P < 0.05). Interactions of sex and GH alleles were analyzed. In heifers, allele A gave higher carcass weight, daily carcass gain, rib eye area, rib thickness, subcutaneous fat thickness, and BMS while allele B gave greater rib eye area and rib thickness (P < 0.05). Allele C gave higher C18:1 (P < 0.01), MUFA (P < 0.01), USFA percentages (P < 0.05) and MUFA/SFA and USFA/SFA ratios (P < 0.01), and lower C16:0 and SFA percentages (P < 0.05) and MP (P < 0.01). GH gene polymorphism affected carcass traits and fatty acid compositions although the effects were more pronounced in heifers.
Dietary phytase increases the true absorption and endogenous fecal excretion of zinc in growing pigs given a corn‐soybean meal based diet
Authors: CHU Gyo‐Moon , KOMORI Motoharu , HATTORI Ryota , MATSUI Tohru 
Published: October 2008

ABSTRACTWe investigated the effect of dietary phytase on the true absorption and endogenous fecal excretion of zinc (Zn) using 67Zn in growing pigs given a corn‐soybean meal based diet. Ten crossbred barrows were fed the control diet containing 90‐mg/kg Zn, 2.3‐g/kg phytate‐phosphorus and 3.7‐g/kg non‐phytate‐phosphorus or the phytase diet containing similar amounts of Zn and phytate‐phosphorus, and 1.4‐g/kg non‐phytate‐phosphorus with 750‐PU/kg phytase for 12 h/day. On day 6, the pigs were given 200 g of the corresponding diet labeled by 67Zn for 2 h. We measured feed intake, fecal Zn concentration and 67Zn abundance for the determination of apparent absorption, true absorption and endogenous fecal excretion of Zn. Although the apparent absorption of Zn did not significantly differ between the dietary groups, the phytase group had significantly more (P < 0.05) true absorption of Zn than the control group. The endogenous fecal excretion of Zn tended to be more (P = 0.07) in the phytase group than in the control group. These results suggest that dietary phytase improves Zn bioavailability through increasing the true absorption of Zn in growing pigs, which results in stimulating the endogenous fecal excretion of Zn when dietary Zn satisfies its requirement.
Characterization of bacteriocin produced by Streptococcus bovis J2 40‐2 isolated from traditional fermented milk ‘Dahi’
Authors: RASHID Harun‐ur , TOGO Kaname , UEDA Minoru , MIYAMOTO Taku 
Published: October 2008

ABSTRACTA bacteriocin‐producing strain Streptococcus bovis J2 40‐2 was isolated from traditional fermented milk ‘Dahi’ in Bangladesh. Despite its narrow antimicrobial spectrum, it showed strong antimicrobial activity against extremely challenging and problematic organisms in foods, such as Listeria monocytogenes. Bacteriocin was sensitive to several proteolytic enzymes and showed antimicrobial activity over a wide pH range of 2.0–10.0. It was stable when heated to 110°C for 20 min, but lost 25% of its activity when heated to 121°C for 15 or 20 min. Optimum bacteriocin production (5600 AU/mL) was achieved when the strain was cultured at 37°C for 24 h in MRS medium rather than in TYLG, GM17, or skim milk medium. Bacteriocin was partially purified by ammonium sulfate precipitation (80% saturation), dialysis (cut‐off MW: 3500) and gel filtration chromatography. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis revealed that bacteriocin had a molecular weight of approximately 4.5 kDa.
Effect of monomer treatment and polymerisation methods on the bond strength of resin teeth to denture base material
Authors: Barbosa Debora Barros , Monteiro Douglas Roberto , Barão Valentim Adelino Ricardo , Pero Ana Carolina , Compagnoni Marco Antonio 
Published: October 2008

Background:  The fracture between acrylic denture base material and artificial teeth is a common clinical occurrence in dental prosthodontic practice.Objective:  To evaluate the bond strength between acrylic resins and resin denture teeth when submitted by two protocols of monomer liquid application on the tooth surface and using different polymerisation methods.Material and methods:  Microwave‐polymerised (Onda‐Cryl), heat‐polymerised (Clássico) and autopolymerising (Jet) acrylic resins and a brand of resin denture teeth (Biotone) were used. The acrylic resins were polymerised according to the cycles: (A) microwave – fast cycle, Onda‐Cryl; (B) microwave – long cycle, Onda‐Cryl; (C) microwave – manufacturer’s cycle, Onda‐Cryl; (T) water bath – long cycle, Clássico and (Q) bench polymerisation cycle, Jet. Thirty specimens were prepared for each polymerisation method; 10 were packed with acrylic resin after 60 s of monomer liquid application on the tooth surface, 10 after 180 s and 10 without any monomer liquid application. For the purpose of the study, a shear test was used. anova and Tukey tests were performed to identify significant differences (α = 0.05).Results:  The highest bond strength values were found for monomer surface treatments, regardless of the polymerisation cycles. The highest significant values were found for cycles B (15.4 ± 1.8 MPa), C (11.9 ± 4.9 MPa) and T (15.4 ± 2.6 MPa) for non‐treated and 60 s methylmethacrylate treated groups. Comparing the monomer liquid treatment, they did not differ significantly (p > 0.05), except for cycle A (p < 0.05).Conclusion:  Chemical treatment using monomer on the tooth surface prior to the acrylic resin packing improved the bond strength between resin denture tooth and acrylic resin, regardless of monomer liquid treatment protocols. The microwavable resin, polymerised by fast cycle and autopolymerising resin should be avoided for processing denture and denture repairs, respectively.
Evaluation and donor site morbidity of tibial bone graft in sinus lift operation
Authors: Miranda S.L. , Fonseca A.P.Z. , Guarnieri M. 
Published: October 2008

AbstractAim:  The use of autologous bone is a good option for bone grafts in implantology. The purpose of this study was to present advantages in harvesting the proximal tibial cancellous bone as an alternative in the sinus floor lifting procedure, seeking future installation of osseointegraded implants.Materials and methods:  Between 2002 and 2006, 19 patients were attended, with age between 41 and 77, being 9 males and 10 females, in order to lift the maxillary sinus floor. The approach to the donor site was medial, because of the fact that this method brings minor risk of injury to anatomical structures.Results:  All cases allowed the installation of osseointegraded implants. Only minor complications occurred, such as local ecchymosis and ecchymosis extending to foot. In all cases, ambulation was immediate.Conclusions:  A good amount of bone could be harvested; scarce pain symptomatology was detected, and risk of complications could be considered very low in the donor site.
Presentation of a plasmablastic lymphoma in a human immunodeficiency virus‐positive patient after administration of an inferior alveolar dental block
Authors: McKenna Gerald , Manton Sarah 
Published: October 2008

AbstractHuman immunodeficiency virus (HIV) is a serious worldwide healthcare problem with implications for all healthcare workers. The reported oral manifestations of the disease are numerous and have been categorised according to the strength of their association with HIV infection. Oral non‐Hodgkin's lymphoma is strongly associated with HIV infection, and an increased incidence of such neoplasms is widely reported. This case report details the presentation of a rare subcategory of plasmablastic lymphoma in an HIV‐positive patient after administration of an inferior alveolar dental block to facilitate extraction of mandibular teeth. This highly aggressive neoplasm is a large B‐cell lymphoma with a predilection for the oral cavity. Unfortunately, the prognosis for such a tumour is poor as detailed in this case.
Palatal mucormycosis: a rare clinical dilemma
Authors: Shetty S.R. , Punnya V.A. 
Published: October 2008

AbstractMucormycosis, caused by saprophytic fungi of class zygomycetes, is a rare opportunistic, fungal infection which has a rapidly progressive and fulminant course with fatal outcome. Rarity of the disease frequently impairs diagnosis leading to worsened prognosis for patients. The present article describes a case of mucormycosis affecting the palate in a patient with uncontrolled diabetes mellitus which was successfully treated with combined therapy. The aim of this article was to emphasise the occurrence of this entity in oral cavity especially in a background of immunosuppresion and to discuss the importance of its early diagnosis to allow prompt and aggressive therapy.
Replacement of congenically missing maxillary permanent canine with a zirconium oxide dental implant and crown. A case report from an ongoing clinical study
Authors: Oliva X. , Oliva J. , Oliva J.D. 
Published: October 2008

AbstractThis article describes a case report of an ongoing clinical investigation with zirconium oxide (ZrO2) dental implants. The patient was a young female who was missing an upper right canine. A one‐piece (implant + abutment) ZrO2 implant (CeraRoot) with acid‐etched surface (ICE surface®) was implanted. The preoperative situation was ideal in terms of soft tissue and bone preservation, and the surgery was done transmucosally without having to raise a flap. The soft tissue healed successfully around the implants within few days. The case was finally restored with a ZrO2 crown (CeraCrown) and ceramic characterisation.
Osteodystrophy and brown tumour causing localised jaw enlargement
Authors: De Lacerda Júlio César Tanos , Gomes Carolina Cavaliéri , E Oliveira Carla da Silveira , Gomez Ricardo Santiago 
Published: October 2008

AbstractChronic renal disease is characterised by progressive and irreversible loss of renal function. A number of systemic and oral complications are associated with chronic renal disease. We report a rare case of jaw enlargement caused by the simultaneous presence of osteitis fibrosis and brown tumour secondary to hyperparathyroidism. Both conditions are related to increased levels of parathyroid hormone and dental surgeons should be aware of this important complication associated with chronic renal disease.
A classification system of mandibular prognathism
Authors: Cho H.J. , Nguyen T. 
Published: October 2008

AbstractAim:  To provide a classification system of mandibular prognathism (MP) as a guide for proper diagnosis and treatment planning of the various forms of skeletal discrepancies seen in mandibular prognathism and to a communication tool among orthodontists and surgeons.Materials and Methods:  Six different types of MP were presented. Recommended surgical procedures for each MP categories were presented. Twenty‐three consecutive MP patients were evaluated by a panel of three orthodontists. The panel categorized each patient according to Cho's MP classification system. The recommended surgery by the MP classification system and actual surgery performed for each were compared.Result:  The recommended surgical procedure(s) respective to the MP category of each individual corresponded to the actual surgery performed with exception of a few individuals.Conclusion:  This MP classification system proved to be a good diagnostic tool and led to an appropriate orthodontic and surgical treatment plan.
Different topoisomerase IIa protein expression patterns affect prognosis in tongue squamous cell carcinoma: a quantitative digital image analysis study
Authors: Machaira A. , Tsiambas E. , Goula K. , Chrysomali E. , Nikolatou O. , Manaios L. , Rapidis A.D. , Karameris A. , Patsouris E. 
Published: October 2008

AbstractAim:  Topoisomerases represent a class of nucleic enzymes involved in the DNA replication, transcription and also chromosome topological formation. Topoisomerase IIa (Topo Iia – gene location 17q21) inhibition promotes cell death and for this reason, it is a target for specific chemotherapy (anthracyclines, podophyllotoxines). Our aim was to investigate the potential prognostic significance of its protein expression in squamous cell carcinomas of the tongue (TSCCs) based on a quantitative, digital image analysis method.Materials and methods:  We analysed immunohistochemically eighty‐seven (n = 87) archival, primary TSCCs using a monoclonal anti‐Topo IIa antibody. A computerised image analysis assay was applied for the evaluation of the results [nuclear labelling index (NLI)]. Survival analysis was also performed.Results:  Topo IIa protein was overexpressed in 48/87 (55.1%) cases. High NLI values were detected in 17/48 (35.4%), whereas moderate levels of expression were detected in 31/48 (64.6%) cases. Statistical significance was not assessed correlating overall protein expression to grade or stage of the examined tumours (P = 0.88, P = 0.73, respectively), but only to the anatomical region (P = 0.04). Cox regression analysis showed that Topo IIa and also the size of the tumours were strongly associated to the survival of the patients (P = 0.01, P = 0.02, respectively).Conclusions:  Topo IIa protein overexpression is a frequent event in TSCCs. Topo IIa may be used as a prognostic factor and also as a basis for targeted chemotherapy strategies in TSCCs. Furthermore, digital image analysis improves the quantification of immunohistochemical stains in an accurate and fast way.
Development of 14 minisatellite markers for the citrus canker bacterium, Xanthomonas citri pv. citri
Authors: NGOC L. BUI THI , VERNIERE C. , VITAL K. , GUERIN F. , GAGNEVIN L. , BRISSE S. , AH‐YOU N. , PRUVOST O. 
Published: October 2008

AbstractWe screened the genome of Xanthomonas citri pv. citri strain 306 for tandem repeats. A multiplex polymerase chain reaction protocol was used to assess the genetic diversity of 239 strains of X. citri pv. citri from Asia. The total number of alleles per locus ranged from three to 20. Using pooled data sets, 223 different haplotypes were identified. Successful amplifications were obtained at most loci for seven other X. citri pathovars. This typing scheme is expected to be useful at different spatial scales for population studies of pathovars of X. citri, several of which cause plant diseases of economic importance.
Characterization of microsatellite loci in the subsocial spider Stegodyphus lineatus (Araneae: Eresidae)
Authors: BILDE T. , TUNI C. , CARIANI A. , SANTINI A. , TABARRONI C. , GAROIA F. , GOODACRE S. L. 
Published: October 2008

Abstract Stegodyphus lineatus spiders live in groups consisting of closely related individuals. There appears to be no discrimination against related individuals as mates but females mate multiply, despite the fact that matings are shown to carry a cost. We have developed eight polymorphic dinucleotide microsatellite markers that allow us to assess levels of heterozygosity and relatedness among individuals of this species. These molecular markers are likely to prove highly effective tools for estimating levels of inbreeding and thus allow us to test hypotheses about the relationships between social structure, mating strategies and inbreeding avoidance.
Development and characterization of 12 polymorphic microsatellite loci in the American dog tick (Dermacentor variabilis)
Authors: DHARMARAJAN GUHA , FIKE JENNIFER A. , BEASLEY JAMES C. , RHODES JR OLIN E. 
Published: October 2008

AbstractTwelve polymorphic microsatellite loci were developed for the American dog tick (Dermacentor variabilis), an important vector of infectious diseases in humans and animals. Four multiplexed panels comprising the loci were developed and 45 ticks collected from two raccoons (Procyon lotor) were genotyped. The number of alleles per locus ranged from nine to 30, and single locus heterozygosities ranged from 0.18 to 0.93. Data generated using these markers will further our understanding of factors affecting gene flow in D. variabilis, thus helping to elucidate the transmission dynamics of diseases associated with this vector.
Characterization of 13 microsatellites for Lahontan cutthroat trout (Oncorhynchus clarki henshawi) and cross‐amplification in six other salmonids
Authors: ROBINSON M. L. , KIRCHOFF V. S. , PEACOCK M. M. 
Published: October 2008

AbstractThirteen newly developed tri‐ and tetranucleotide repeat microsatellite markers were developed for Lahontan cutthroat trout (Oncorhynchus clarki henshawi), a threatened subspecies endemic to the Lahontan hydrographic basin in the western USA. These loci are highly polymorphic with five to 30 alleles per locus and observed heterozygosities ranging from 0.4 to 0.7. Cross‐species amplification of these markers was most successful in the closely related rainbow trout, Oncorhynchus mykiss, with only three loci amplifying in brown trout, Salmo trutta. Nonoverlapping allelic distributions for many of these loci among the six salmonid species screened suggest these markers may be useful for hybrid determination.
Identification and characterization of microsatellite markers from the starfish Asterina pectinifera expressed sequence tags
Authors: LI QI , NI GANG , LIU JUN , LI JINGJING , KONG LINGFENG 
Published: October 2008

AbstractThe starfish (Asterina pectinifera) is a common species widely distributed along the coasts of East Asia. Nevertheless, little information is currently available about the genetic characteristics of native populations. In this survey, the first set of 14 microsatellite markers for the starfish was developed from expressed sequence tag databases. They were characterized using 40 samples. Number of alleles per locus ranged from three to 14. The observed and expected heterozygosities ranged from 0.300 to 0.825 and from 0.479 to 0.919, respectively. These informative microsatellite markers will be useful in studies of population genetic structure for this species.
Is cortical root colonization required for carbon transfer to arbuscular mycorrhizal fungi? Evidence from colonization phenotypes and spore production in the reduced mycorrhizal colonization (rmc) mutant of tomato
Authors: Manjarrez Maria , Smith F. Andrew , Marschner Petra , Smith Sally E. 
Published: October 2008

For the first time, the phenotypes formed in the reduced mycorrhizal colonization (rmc) Solanum lycopersicum L. (tomato) mutant with different arbuscular mycorrhizal (AM) fungi were used to explore the potential of different fungal structures to support development of external fungal mycelium and spores. The life cycle of AM fungi with rmc was followed for up to 24 weeks. Results showed that production of external mycelium was slight and transitory for those fungi that did not penetrate the roots of rmc (Pen–) ( Glomus intraradices DAOM181602 and Glomus etunicatum ). For fungi that penetrated the root epidermis and hypodermis (Coi–, Glomus coronatum and Scutellospora calospora ) the mycelium produced varied in size, but was always smaller than with the wild-type 76R. Spores were formed by these fungi with 76R but not with rmc. The only fungus forming a Myc+ phenotype with rmc, G. intraradices WFVAM23, produced as much mycelium with rmc as with 76R. We observed lipid accumulation in hyphae and vesicles in both plant genotypes with this fungus. Mature spores were formed with 76R. However, with rmc, spores remained small and (presumably) immature for up to 24 weeks. We conclude that significant carbon transfer from plant to fungus can occur in Coi– interactions with rmc in which no cortical colonization occurs. We speculate that both carbon transfer and root signals are required for mature spores to be produced.
Ecology of the 2004 morel harvest in the Rocky Mountain Forest District of British Columbia
Authors: Winder Richard S. , Keefer Michael E. 
Published: October 2008

In the Rocky Mountain Forest District of British Columbia, a dramatic series of fires occurred during 2003, setting the stage for an abundant morel crop in the following year. During 2004, the abundance of post-fire morels ( Morchella spp.) was measured and the plant community associated with morel production was characterized. Morel production averaged 6473 ± 2721 morels·ha–1in five burnt forests that were surveyed. Production ranged from 1702·ha–1at Plumbob Mountain to a significantly higher 16827·ha–1in the Kootenay National Park, where the highest level of duff consumption (71%) was also observed. Several plant species had high importance in morel habitat, and were also associated with above-average morel abundance: Chamerion angustifolium (L.) Holub, Arnica cordifolia Hook., Erythronium grandiflorum Pursh, Spiraea betulifolia Pallas subsp. lucida (Dougl. ex Greene) Taylor and MacBryde, Menziesia ferruginea Sm., Rosa acicularis Lindl. subsp. sayi (Schwein.) W.H. Lewis, Pinus contorta Dougl. ex Loud. var. latifolia Engelm., Abies lasiocarpa (Hook.) Nutt., and Picea glauca (Moench) Voss × engelmannii (Torr. & Gray ex Hook.) Brayshaw. Compositae and Vaccinium spp. were important species when considered as groups. Grass species, including Calamagrostis rubescens Buckl., were more proximate to morel-free plots. The characteristics of morel habitats observed in this study may be useful in future management of the resource, through conservation of habitat, management of prescribed burning, and postponement of salvage logging in potentially highly productive areas.
Effects of zucchini yellow mosaic virus infection on the subcellular distribution of glutathione and its precursors in a highly tolerantCucurbita pepocultivar
Authors: Zechmann Bernd , Müller Maria 
Published: October 2008

Glutathione (γ-glutamyl-cysteinyl-glycine) is an antioxidant that plays important protective roles during pathogen attack. Glutathione synthesis is a highly compartment-specific pathway and relies on the supply of its precursors cysteine, glutamate, and glycine. To study the importance of glutathione in the development of tolerance and resistance, glutathione and its precursors were quantified with cytohistochemical methods in a highly tolerant cultivar (‘Quine’) of Cucurbita pepo L. during zucchini yellow mosaic virus (ZYMV) infection. Glutathione concentrations were strongly increased in almost all cell compartments of younger leaves (up to 247% in peroxisomes), older leaves (up to 73% in the cytosol), and roots (up to 104% in nuclei) during ZYMV infection. In addition, glutathione precursor concentrations were generally unchanged or increased in all cell compartments of younger leaves and roots, indicating that glutathione synthesis in these organs was not limited by low levels of glutathione precursors. The lower increase of glutathione in older leaves could be caused by strongly decreased levels of glycine in plastids (42%) and the cytosol (53%) during ZYMV infection, as they are the main centers for glutathione synthesis. Nevertheless, as the increase in glutathione was in general much higher than that observed in a highly susceptible cultivar in previous studies, we can conclude that elevated glutathione concentrations can be strongly correlated with the development of tolerance during ZYMV infection.