bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow cytometric determination of PMCA‐mediated Ca2+‐extrusion in individual red blood cells
Authors: de Jong Kitty , Kuypers Frans A. 
Published: June 2007

AbstractBackground:Differences among red blood cells in the activity of the plasma membrane Ca2+‐ATPase (PMCA) can impact cell signaling and survival. However, no method has been reported that measures this activity directly in individual cells.Methods:We have designed a novel assay for PMCA activity that uses the fluorescent Ca2+‐reporter Fluo4 and flow cytometric analysis. The method recognizes the extrusion of Ca2+ from the cell after a short Ca2+‐loading pulse, which avoids the problem of ATP depletion and ascertains activity at Vmax capacity.Results:Our assay is responsive to known PMCA inhibitors, and while not intended for quantitative kinetic analysis of Ca2+‐pumping, it can be used to determine qualitative differences between red blood cell populations that vary in PMCA activity. Using this assay, we confirmed that a normal red blood cell population shows heterogeneity with respect to the PMCA Vmax.Conclusion:We report a novel assay of PMCA activity in red blood cells that can provide qualitative information on PMCA activity in individual cells. © 2007 International Society for Analytical Cytology
Laminin and fibronectin modulate inner ear spiral ganglion neurite outgrowth in an in vitro alternate choice assay
Authors: Evans Amaretta R. , Euteneuer Sara , Chavez Eduardo , Mullen Lina M. , Hui Elliot E. , Bhatia Sangeeta N. , Ryan Allen F. 
Published: June 2007

AbstractExtracellular matrix (ECM) molecules have been shown to function as cues for neurite guidance in various populations of neurons. Here we show that laminin (LN) and fibronectin (FN) presented in stripe micro‐patterns can provide guidance cues to neonatal (P5) inner ear spiral ganglion (SG) neurites. The response to both ECM molecules was dose‐dependent. In a LN versus poly‐L‐lysine (PLL) assay, neurites were more often observed on PLL at low coating concentrations (5 and 10 μg/mL), while they were more often on LN at a high concentration (80 μg/mL). In a FN versus PLL assay, neurites were more often on PLL than on FN stripes at high coating concentrations (40 and 80 μg/mL). In a direct competition between LN and FN, neurites were observed on LN significantly more often than on FN at both 10 and 40 μg/mL. The data suggest a preference by SG neurites for LN at high concentrations, as well as avoidance of both LN at low and FN at high concentrations. The results also support a potential model for neurite guidance in the developing inner ear in vivo. LN, in the SG and osseus spiral lamina may promote SG dendrite growth toward the organ of Corti. Within the organ of Corti, lower concentrations of LN may slow neurite growth, with FN beneath each row of hair cells providing a stop or avoidance signal. This could allow growth cone filopodia increased time to sample their cellular targets, or direct the fibers upward toward the hair cells. © 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2007
Effect of lipid supplements on ruminal biohydrogenation intermediates and muscle fatty acids in lambs
Authors: Bessa Rui J. B. , Alves Susana P. , Jerónimo Eliana , Alfaia Cristina M. , Prates José A. M. , Santos‐Silva José 
Published: June 2007

AbstractThe objective was to characterize the fatty acid (FA) composition of lamb meat with emphasis on biohydrogenation intermediates (BI) induced by dietary sunflower and linseed oil and to test if a synergistic effect on meat trans‐11 18:1 and cis‐9,trans‐11 18:2 concentrations could be obtained with a blend of both oils. Thirty two lambs were assigned to four groups and fed for 6 weeks one of the following diets: pelleted dehydrated lucerne (Control); and Control supplemented with 7.4% of sunflower oil (SF), linseed oil (LS) or a blend of sunflower and linseed oils (2 : 1 vol/vol) (SFLS). Longissimus thoracis muscles were analyzed for FA. LS increased n‐3 PUFA due to contribution of 18:3n‐3 but not of very long n‐3 PUFA. Total conjugated linoleic acids were similar in oil‐supplemented lambs, but the cis‐9,trans‐11 18:2 was higher with SF than with LS. No synergistic effects on trans‐11 18:1 or cis‐9,trans‐11 18:2 were observed when both oils were fed together. Oil supplementation increased the concentrations of most BI in meat. However, the BI patterns were different for LS and SF. Some FA were only found in lambs fed linseed oil, including the unusual cis‐12,cis‐15 18:2 which is proposed as a new intermediate of the 18:3n‐3 biohydrogenation pathway.
Characterization of olive paste volatiles to predict the sensory quality of virgin olive oil
Authors: García‐González Diego L. , Tena Noelia , Aparicio Ramón 
Published: June 2007

AbstractOne of the main challenges that virgin olive oil producers face today is an accurate prediction of the sensory quality of the final product prior to the milling of the olives. The possibility that olive paste aroma can be used as a predictive measurement of virgin olive oil quality is studied in this paper. The study was centered on distinguishing the aroma of olive pastes that produced virgin olive oils without sensory defects from the aroma of olive pastes the virgin olive oils of which showed sensory defects. Olive pastes were analyzed by solid‐phase microextraction‐gas chromatography and a sensor system based on metal oxide sensors. Forty‐four volatile compounds were identified in olive pastes, all of them being also present in virgin olive oil. Six volatile compounds – acetic acid, octane, methyl benzene, (E)‐2‐hexenal, hexyl acetate and 3‐methyl‐1‐butanol – distinguished both kinds of pastes with only five misclassified samples. Five metal oxide sensors were able to classify the olive pastes with only two erroneous classifications.
Numerical simulations of the steady Navier–Stokes equations using adaptive meshing schemes
Authors: Ju Lili , Lee Hyung‐Chun , Tian Li 
Published: June 2007

AbstractIn this paper, we consider an adaptive meshing scheme for solution of the steady incompressible Navier–Stokes equations by finite element discretization. The mesh refinement and optimization are performed based on an algorithm that combines the so‐called conforming centroidal Voronoi Delaunay triangulations (CfCVDTs) and residual‐type local a posteriori error estimators. Numerical experiments in the two‐dimensional space for various examples are presented with quadratic finite elements used for the velocity field and linear finite elements for the pressure. The results show that our meshing scheme can equally distribute the errors over all elements in some optimal way and keep the triangles very well shaped as well at all levels of refinement. In addition, the convergence rates achieved are close to the best obtainable. Extension of this approach
to three‐dimensional cases is also discussed and the main challenge is the efficient implementation of three‐dimensional CfCVDT generation that is still under development. Copyright © 2007 John Wiley & Sons, Ltd.
Erratum: Validation of the Spanish version of the geriatric depression scale (GDS) in primary care
Authors: Fernández‐San Martín M. I. , Andrade C. , Molina J. , Muñoz P. E. , Carretero B. , Rodríguez M. , Silva A. 
Published: June 2007

AbstractInternational Journal of Geriatric Psychiatry 2002; 17: 279–287DOI: 10.1002/gps.588It has come to our attention that there was an error to one of the author names within this published article. The correct author listing is now published below. We apologise for this anomaly.M. I. Fernández‐San Martín, C. Andrade‐Rosa, J. D. Molina, P. E. Muñoz, B. Carretero, M. Rodríguez and A. Silva
Lack of effects between rupatadine 10 mg and placebo on actual driving performance of healthy volunteers
Authors: Vuurman Eric , Theunissen Eef , van Oers Anita , van Leeuwen Cees , Jolles Jelle 
Published: June 2007

AbstractIntroductionRupatadine fumarate is a potent, selective, histamine H1‐receptor antagonist and PAF inhibitor with demonstrated efficacy for the relief of allergic rhinitis. Rupatadine does not easily cross the blood–brain barrier and is believed to be non‐sedating at therapeutic doses. Consequently, rupatadine should show no impairment on car driving.ObjectiveThis study compared the acute effects of rupatadine, relative to placebo and hydroxyzine (as an active control), on healthy subjects' driving performance.MethodsTwenty subjects received a single dose of rupatadine 10 mg, hydroxyzine 50 mg, or placebo in each period of this randomized, double‐blind, three‐way crossover study. Two hours postdosing, subjects operated a specially instrumented vehicle in tests designed to measure their driving ability. Before and after the driving tests ratings of sedation were recorded.ResultsThere was no significant difference between rupatadine and placebo in the primary outcome variable: standard deviation of lateral position (SDLP); however, hydroxyzine treatment significantly increased SDLP (p < 0.001 for both comparisons). Objective (Stanford sleepiness scale) and subjective sedation ratings (Visual Analogue Scales) showed similar results: subjects reported negative effects after hydroxyzine but not after rupatadine.ConclusionRupatadine 10 mg is not sedating and does not impair driving performance. Copyright © 2007 John Wiley & Sons, Ltd.
Application of hydrophobic resin adhesives to acid‐etched dentin with an alternative wet bonding technique
Authors: Sadek Fernanda T. , Pashley David H. , Nishitani Yoshihiro , Carrilho Marcella R. , Donnelly Adam , Ferrari Marco , Tay Franklin R. 
Published: June 2007

AbstractHydrophilic dentin adhesives are prone to water sorption that adversely affects the durability of resin–dentin bonds. This study examined the feasibility of bonding to dentin with hydrophobic resins via the adaptation of electron microscopy tissue processing techniques. Hydrophobic primers were prepared by diluting 2,2‐bis[4(2‐hydroxy‐3‐methacryloyloxy‐propyloxy)‐phenyl] pro‐ pane/triethyleneglycol dimethacrylate resins with known ethanol concentrations. They were applied to acid‐etched moist dentin using an ethanol wet bonding technique that involved: (1) stepwise replacement of water with a series of increasing ethanol concentrations to prevent the demineralized collagen matrix from collapsing; (2) stepwise replacement of the ethanol with different concentrations of hydrophobic primers and subsequently with neat hydrophobic resin. Using the ethanol wet bonding technique, the experimental primer versions with 40, 50, and 75% resin exhibited tensile strengths which were not significantly different from commercially available hydrophilic three‐step adhesives that were bonded with water wet bonding technique. The concept of ethanol wet bonding may be explained in terms of solubility parameter theory. This technique is sensitive to water contamination, as depicted by the lower tensile strength results from partial dehydration protocols. The technique has to be further improved by incorporating elements of dentin permeability reduction to avoid water from dentinal tubules contaminating water‐free resin blends during bonding. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Delivery of plasmid IGF‐1 to chondrocytes via cationized gelatin nanoparticles
Authors: Xu Ximing , Capito Ramille M. , Spector Myron 
Published: June 2007

AbstractThe objective of the present study was to investigate the use of gelatin and cationized‐gelatin nanoparticles for the nonviral delivery of the plasmid DNA encoding for insulin‐like growth factor (IGF)‐1 to adult canine articular chondrocytes in vitro; plasmid for enhanced green fluorescence protein (EGFP) was used as a marker gene. The spherical cationized gelatin nanoparticles were on average 172 nm in diameter, compared with the often ellipsoid‐shaped unmodified (noncationized) gelatin particles that generally appeared to be 10 μm to greater than 20 μm in length. The zeta potential of the positively charged cationized gelatin nanoparticles containing the plasmid was around 20 mV compared with about 2 mV for the unmodified gelatin particles. There was no noticeable fluorescence from the cells treated with the nanoparticles prepared with the original (noncationized) gelatin particles containing the pEGFP. In contrast, numerous cells in the group transfected with the cationized gelatin‐pEGFP nanoparticles were found to fluoresce demonstrating the transfection of the cells. There was five‐fold elevation in the amount of IGF‐1 produced by the cells treated with the cationized gelatin nanoparticles containing the IGF‐1 plasmid compared with the unmodified (noncationized) gelatin particles. There was a clear effect of varying the weight ratio of plasmid IGF‐1 in the cationized gelatin nanoparticles on the IGF‐1 in the medium of cells exposed to the nanoparticles for 5 h. A peak in the amount of released IGF‐1 was detected at a gelatin:IGF‐1 weight ratio of 250:1. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Ligament repair: A molecular and immunohistological characterization
Authors: Roseti L. , Buda R. , Cavallo C. , Desando G. , Facchini A. , Grigolo B. 
Published: June 2007

AbstractThe anterior cruciate ligament (ACL) is the most commonly injured tissue of the human knee. Its poor ability to regenerate after injury represents a challenge to ligament tissue engineering. An understanding of the molecular composition of the structures used for its repair is essential for clinical assessments and for the implementation of tissue engineering strategies. The objective of this study was to evaluate, both at gene and protein levels, the expression of characteristic molecules in human ACL, patellar, semitendinosus and gracilis tendons and in the ligament reconstructed with patellar or semitendinosus and gracilis tendons. We demonstrated that primary ACL and tendon tissues all express collagen I, II, Sox‐9, tenascin‐C and aggrecan. Collagen X expression was detected at very low levels or undetectable. Cathepsin B, MMP‐1 and MMP‐13 were expressed at higher levels in the ACL reconstructed by the two tendons, showing that a remodeling process occurs during “ligamentization”. Both our molecular and immunohistochemical evaluations did not reveal significative differences between the tendons and ligaments analyzed. However, ACL reconstructed with semitendinosus and gracilis tendon seems to present a higher expression of collagen type II when compared to that reconstructed with patellar tendon. This study could give a reasonable identification of genetic and protein markers specific to tendon/ligament tissues and be helpful in testing tissue engineering approaches for ACL reconstruction. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Thermoresponsive degradable poly(ethylene glycol) analogues
Authors: Wang Na , Dong Anjie , Radosz Maciej , Shen Youqing 
Published: June 2007

AbstractThermoresponsive polymers have many biomedical applications, but their nondegradability limits their in vivo applications. Herein, we report a new type of degradable thermoresponsive polymers—degradable poly (ethylene glycol) analogues (DPEGs) having lower critical solution temperatures (LCSTs) ranging 10–50°C. DPEGs were synthesized by condensation polymerization of PEG‐di(meth)acrylates (PEGDA or PEGDMA) with dithiols. Their LCSTs could be easily tuned by the PEG‐chain length and the types of the double bond in the PEG monomers and dithiols. Long PEG chain and the presence of hydrophilic groups in the dithiol monomer increased the LCST of the resulting DPEG. Crosslinking DPEG chains produced thermoresponsive hydrogels. The hydrogels prepared by the end‐capping method maintained the thermoresponsive properties of the linear DPEG. The degradable thermoresponsive DPEGs and their hydrogels have great potentials for in vivo biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
RFID update
Authors: Higgins Leslee N. , Cairney Tim 
Published: June 2007

AbstractRadio frequency identification (RFID) tags have been around for a while now. Wal‐Mart and the Department of Defense mandated their use four years ago. Since then, the cost of RFID tags has dropped. But several sectors have still been slow to adopt them. Is this delay acceptable, or will delayed adoption harm a company? The authors take a close look at these and other critical RFID issues. © 2007 Wiley Periodicals, Inc.
Developmental stage affects eggshell‐breaking strength in two ground‐nesting birds: the partridge (Alectoris rufa) and the quail (Coturnix japonica)
Authors: Castilla Aurora M. , Herrel Anthony , Díaz Guillém , Francesch Amadeu 
Published: June 2007

AbstractWe examined the relationship between embryo development and egg hardness in two ground nesting bird species, the red‐legged partridge (Alectoris rufa; n = 165 eggs) and the quail (Coturnix japonica; n = 148 eggs). For both species, we observed a strong effect of developmental stage on egg hardness. Eggs near hatching were significantly weaker than unincubated eggs (partridge: 18 and 23 N, respectively, and the quail 7 and 10 N, respectively). We additionally explored the effect of incubation on egg hardness in a control sample of non‐fertilised quail eggs (i.e., without embryo development). The control eggs maintained in the incubator for the full incubation time (17 days) were significantly harder (7–9 N) than eggs containing fully developed chicks (5–7 N). Thus, the incubation conditions of high temperature and humidity alone seem not to have a significant effect on egg hardness, and support the important effect of calcium uptake. J. Exp. Zool. 307A:471–477, 2007. © 2007 Wiley‐Liss, Inc.
Hereditary Amyloidosis with Progressive Peripheral Neuropathy Associated with Apolipoprotein AI Gly26Arg
Authors: Testro Adam G. , Brennan Stephen O. , Macdonell Richard A.L. , Hawkins Philip N. , Angus Peter W. 
Published: June 2007

Abstract
Liver transplantation (LT) has been reported in only 1 patient with hereditary variant apolipoprotein AI (apoAI) amyloidosis and was associated with a 50% decrease in production of variant apoAI. The potential for this to benefit clinical manifestations of apoAI amyloidosis such as peripheral neuropathy has not been determined. A 59-yr-old Irish-born male with hereditary systemic amyloidosis associated with apoAI Gly26Arg, which was suspected to be the cause of a progressive peripheral neuropathy, along with end-stage renal failure and hepatic dysfunction, underwent hepatorenal transplantation. Evaluation of his clinical outcome included serial neurological examinations and nerve conduction studies. The proportion of variant apoAI in the plasma was estimated before and after LT. Hepatorenal transplantation was successful, with the plasma concentration of variant apoAI decreasing by over 50% after transplantation and this was associated with progressive resolution of the patient's neuropathic symptoms and a significant and sustained improvement in electrophysiological parameters. In conclusion, liver transplantation reduces production of the amyloid fibril precursor protein to a degree that can facilitate net regression of amyloid deposits, which may aid recovery of end-organ damage such as that seen in peripheral neuropathy. Liver Transpl 13:1028–1031, 2007. © 2007 AASLD.
The bonded debt of 207 cotoes as at January 1, 1925
Authors: Rightor C. E. 
Published: June 2007

AbstractThe report on bonded debts of cities, prepareddd by Mr. Rightor and the Detroit Bureau of Governmental Research, is now an annual affair. Comparison is now possible whith the tables, published in the Review for May, 1923, and June, 1924.
Sketches of American mayors. I. John F. Hylan of New York
Authors: Epexegeticus  
Published: June 2007

AbstractThis is the first of a series of articles upon some famous municipal executives. Surely thew personalities and methods deserve as close study as the charter provisions under which they operate. Later some city managers may be added (see NATIONAL MUNICIPAL REVIEW for December for a story on City Manager Hopkins of Cleveland).John F. Hylan, a poor boy, born and reared in a country village, was snatched from obscurity to be elected mayor of the nation's largest city and re‐elected by an enormous plurality. He turned his most vocal and vitriolic enemies into political assets and last a third term because Tammany, fearing perhaps that he was growing too powerful, abandoned him. What were the human factors attending his rise and fall?
Rising cost of state government. Popular theories versus fiscal facts
Authors: Heer Clarence 
Published: June 2007

AbstractThe popular theory that state governments are indulging in orgies of spending does not square with the facts. The author here analyzes New York state expenditures from 1917 to 1923. He reduces the 1923 expenditures to the price level of 1917 and finds that 44 percent of the increase is due to higher price of services and material which the stale buys; 13 percent is due to substitution of pay‐as‐you‐go for borrowings; 23 percent to unusual conditions over which the legislature had no control; only 20 percent can be called optional, but even this portion went for purposes which no one can reaaonably question.
OUR AMERICAN MAYOURS. VII. W. Freeland kendrick of philadelphia
Authors: Henry Walnut T. 
Published: June 2007

AbstractMayor Kendrick is not a reformer, nor even a distinguished machine politician. As an inveterate “joinre” of secret societies, he owes much to his famous smile. He has kept peace with the Republican organization which elected him, bu the will be best remembered for his nerveracking experience with General Butler and the ill–fated Sesqui.
“Standards of financial administration” defended
Authors: Upson Lent D. , Rightor C. E. 
Published: June 2007

AbstractWe all covet success, and most of us aspire to efficiency and economy in our practical undertaking, but few of us can agree on the criteria which measure success or efficiency. In the supplement to our February number, Messrs. Upson and Rightor, although aware that few would agree with them, undertook the courageious task of sponsoring certain standards of measurement of a city's financial administration. In the following letter to the editor they make reply to Francis Oakey's critical review which appeared in the “American city” for May.
The Pittsburgh consolidation charter
Authors: Miller Joseph T. 
Published: June 2007

AbstractThis is a reply to the article by Martin L. Faust which appeared in our August number. Mr. Faust criticized the charter and the tactics of the commission of which Mr. Miller is the head. The charter submitted by the commission was radically amended by the legislature and failed to secure the necessary two‐thirds vote in a majority of the municipalities of the county at the election on June 25.
The new york telephone rate decision
Authors: Gold Nathaniel 
Published: June 2007

AbstractNew York state awoke late in January to he imminence of radical increases in telephone rates effective as of february 1. On January 31 the public service commission oodered a slight reduction in the charegs announced by the company. The effect o these rates will be observed for a time, after which the struggle to fix final charges will be resumedThe present situation follows ten yeears of litigation before commission, special master adn court. The length to which the trial has dragged out and the extreme claims in valuation point to the necessity of improved methods of valuation