bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of novel anti-biofilm agent TiO2@GO for reducing the formation of Streptococcus mitis biofilm on teeth
Authors: Ali Md. Romzan , Hassan Md. Sakib , Ridoy Dipto Debnath , Mozumder Prity Lata , Bacchu Md. Sadek , Das Shilpi , Moon Md. Eashanul Karim , Anannya Tamanna Jerin , Hasan Tamim , Aly Mohamed Aly Saad , Akter Selina , Khan Md. Zaved Hossain 
Published: April 2024

Abstract

Treatment of global infectious diseases is becoming trickier due to the biofilm formation of bacteria. A novel therapeutic approach is urgently required to address the problems posed by pathogens' persistent infections linked with biofilms. Oral biofilm caused by Streptococcus mitis may generate several dental health issues such as dental caries and periodontal disease. Here, a novel nanomaterial (TiO2@GO) had proposed as an anti-biofilm agent to hinder the formation of biofilm of S. mitis. TiO2 was synthesized by following the sol-gel method and attached with GO through the Teflon line reactor-assisted method. The interaction of TiO2@GO was confirmed through scanning electron microscopy, Fourier transforms infra-ray spectroscopy, and UV-vis spectroscopy analysis. The inhibition of biofilm was confirmed by measuring the OD of S. mitis after and before treatment through an ELISA reader and performing SEM of teeth after and before treatment. The TiO2@GO inhibited the rate of biofilm formation (89% of biofilm was reduced after 8h of treatment). The TiO2@GO can reduce 70% biofilm formation for 24h which is effective to prevent dental caries. To conclude, the proposed nanomaterial will be the potential agent to inhibit the formation of oral biofilm and treat dental caries.
Factors critical for the successful delivery of telehealth to rural populations: A descriptive qualitative study.
Authors: Barry Rebecca , Green Elyce , Robson Kristy , Nott Melissa 
Published: April 2024

Abstract

Background:
The use of telehealth has proliferated to the point of being a common and accepted method of healthcare service delivery. Due to the rapidity of telehealth implementation, the evidence underpinning this approach to healthcare delivery is lagging, particularly when considering the uniqueness of some service users, such as those in rural areas. This research aimed to address the current gap in knowledge related to the factors critical for the successful delivery of telehealth to rural populations.
Methods:
This research used a qualitative descriptive design to explore telehealth service provision in rural areas from the perspective of clinicians and describe factors critical to the effective delivery of telehealth in rural contexts. Semi-structured interviews were conducted with clinicians from allied health and nursing backgrounds working in child and family nursing, allied health services, and mental health services. A manifest content analysis was undertaken using the Framework approach.
Results:
Sixteen health professionals from nursing, clinical psychology, and social work were interviewed.
Participants mostly identified as female (88%) and ranged in age from 26-65 years with a mean age of 47 years. Three overarching themes were identified: 1) Navigating the role of telehealth to support rural healthcare; 2) Preparing clinicians to engage in telehealth service delivery; and 3) Appreciating the complexities of telehealth implementation across services and environments.
Conclusions:
This research suggests that successful delivery of telehealth to rural populations requires consideration of the context in which telehealth services are being delivered, particularly in rural and remote communities where there are challenges with resourcing and training to support health professionals. Rural populations, like all communities, need choice in healthcare service delivery and models to increase accessibility. Preparation and specific, intentional training for health professionals on how to transition to, and maintain telehealth services is a critical factor for delivery of telehealth to rural populations. Future research should further investigate the training and supports required for telehealth service provision, including who, when and what training will equip health professionals with the appropriate skill set to deliver rural telehealth services.
A novel tissue-engineered corneal epithelium based on ultra-thin amniotic membrane and mesenchymal stem cells
Authors: long qiurong , Huang Chao , Ou Shangkun , Jiang Hao , Zhao Su , Zhang Lingli , Zheng Xueer , Zhang Liying , Gu Hao 
Published: April 2024

Abstract

Purpose: Currently, in vitro cultured corneal epithelial transplantation is effective for treating limbal stem cell dysfunction (LSCD). Selecting carriers is crucial for constructing the corneal epithelium through tissue engineering. This study aimed to effectively simulate the limbal stem cells (LSCs) microenvironment by inoculating mesenchymal stem cells (MSCs) on the basal surface of an ultra-thin amniotic membrane (UAM) to develop an improved tissue-engineered corneal epithelial carrier.
Methods: Traditional AM was modified, and MSCs were inoculated into the UAM stroma to construct a novel UAM-MSC tissue-engineered corneal epithelial carrier. The tissue-engineered corneal epithelium structure and rabbit LSCD model corneas cultured on different carriers were observed through hematoxylin and eosin staining. Cell phenotypes were evaluated through fluorescence staining, WB, and RT-qPCR. Additionally, cell junction genes and expression markers related to anti-neovascularization were evaluated using RT-qPCR. Corneal epithelium cell junctions were observed via an electron microscope. The tissue-engineered corneal epithelium culture medium was analyzed through mass spectrometry.
Results: Tissue-engineered corneal epithelial cells expanded by LSCs on UAM-MSCs had good transparency. Simultaneously, progenitor cell (K14, PNCA, p63) and corneal epithelial (PAX6) gene expression in tissue-engineered corneal epithelium constructed using UAM-MSCs was higher than that in corneal epithelial cells amplified by UAM and de-epithelialized amniotic membrane. Electron microscopy revealed that corneal epithelial cells grafted with UAM-MSCs were closely connected.
Conclusions: The UAM-MSCs vector we constructed can better simulate the limbal microenvironment, and the cultured tissue-engineered corneal epithelium had better transparency, anti-neovascularization properties, closer intercellular connections, and closer resemblance to the natural corneal epithelial tissue phenotype.
Short-term hospitalisation and early readmission of older patients: A case-control study
Authors: Christensen Lasse , Kristensen Kasper , Madsen Mathias Hakon , Danielsen Mathias Brix , Lorenzen Liudmila , Andersen Stig , Jorgensen Martin Gronbech 
Published: April 2024

Abstract

Background Pressure on health care capacity pushes for shorter hospital stays with early discharge from Emergency Departments (ED), leading to a vicious discharge-readmissions circle toxic to the rising number of older patients. Thus, risk factors for early readmission following short-term ED-stays of older patients needs attention.
Methods This case-control study scrutinised hospital records and the prescription database for data on demography, marital status, disability, Charlson comorbidity index (CCI), and redeemed systemic prescription drugs. Patients included were aged ≥70 years, had a primary hospitalisation with discharge from the ED <48 hours, and were readmitted within 7 or 30 days. Exclusion criteria were readmission for specialised treatment and multi-trauma. Data covered hospitalisations for 12-months, and the setting was publicly funded health care.
Results Among 2,343 patients aged ≥70 years with short-term hospitalisation, 266 (11.4%) were readmitted within 30 days, nearly half within 7 days, and one in three with similar diagnosis. Fifty patients were excluded leading to comparison with 432 cases and matched controls. Polypharmacy (≥8 drugs), CCI, marital status, and disability differed markedly with odds ratios for early readmission of 4.3 (95%-CI: 1.4-17, polypharmacy); 2.0 (95%-CI: 1.3-3.0, CCI ≥2); 1.8 (95%-CI: 1.1-2.8, unwed status); 1.6 (95%-CI: 1.0-2.5, disability).
Conclusion Four major risk factors for early readmission of older patients discharged from the ED within 48 hours were identified and relevant as one in three had similar diagnosis at discharge and readmission. These findings should guide the ED attending physician’s focus to prevent early readmission.
Socio-Economic Challenges of Physically Disabled Street Beggars: the case of Debre Markos city . It’s implication for Social protection Policy /directives towards the inclusion of PWDs beggars
Authors: Admas Getachew 
Published: April 2024

Abstract
This study examined the socio-economic challenges of physically disabled street beggars in the case of Debre Markos city. The researcher employed mixed research approaches with a cross-sectional research design. In addition, both probability and non-probability sampling techniques were employed. From the probability sampling techniques, stratified random sampling techniques were used to select the study participants (physical disabled street beggars) and from non-probability sampling techniques, purposive sampling technique was used to select the key informants. And the researcher collected the quantitative data from 133 sample respondents through survey questionnaire which analyzed by descriptive statistics basically through frequency and percentage. While the collected qualitative data through in-depth interview guide, key informant interview guides, and observation checklist were analyzed thematic method of data analysis. The finding reveled that in line with the increasing number of beggars in general and physically disabled street beggars in particular by migrant beggars though large number of them have able bodied (physically strong) and participate other income generating activities, people with different types of physical disabilities practice several socio economic challenges. For instance, lack of income, lack of educational opportunities, negative societal attitude, health, low/no social interaction with their family and community. Due to this fact, physically disabled individuals lives in the street through begging are vulnerable to other challenges.
Grb2 Y160F mutant mimics the wild-type monomeric state dynamics and
the monomer-dimer equilibrium
Authors: Melo Fernando , Casteluci Giovana , Dias Raphael Rodrigues , Martins Ingrid Bernardes Santana , de Almeida Rodrigo Fernandes , Tedesco Jessica Andrade , Caruso Ícaro , de Araujo Alexandre Suman , Itri Rosangela 
Published: April 2024

Abstract
The Growth factor receptor-bound protein 2 (Grb2) participates in early signaling complexes and regulates tyrosine kinase-mediated signal transduction through a monomer-dimer equilibrium. Grb2 dimeric state inhibits signal transduction whereas the monomer promotes signaling downstream. Since Grb2 dimer KD is ~ 0.8 μM, in vitro studies with the monomer are still challenging and require mutations or interaction with phosphotyrosine peptides. However, these mutants were never characterized considering their effects on protein structure and dynamics in solution. Here, we present the biophysical characterization of Grb2Y160F, the first Grb2 mutant to induce protein monomerization without disrupting its native behavior in solution due to net charge modifications or interaction with peptides. We also identified that Grb2Y160F exists in a monomer-dimer equilibrium. Grb2Y160F ability to dimerize implies that different dimerization interfaces might regulate signaling pathways in distinct ways since this mutation has already been reported as functional in other in vitro studies.
Osteonecrosis of the Jaw in Patients with Clear Cell Renal Cell Carcinoma Treated with Targeted Agents: A Case Series and Large-Scale Pharmacovigilance Analysis
Authors: Wang Shuyun , Song Yan , Yin Donghong , Xiao Feng , Xue Lili , Wang Xuyan 
Published: April 2024

Abstract

Objective: We use the public database to describe and evaluate ONJ related to various tyrosine kinase inhibitors (TKIs) and immune checkpoint inhibitors (ICIs) treated for renal cell carcinoma. To provide evidence for optimizing drug administration in the clinic.
Methods: We analyzed spontaneous reports submitted to the FAERS in an observational and retrospective manner between January 2004 and December 2022. Selecting ONJ' adverse events to TKIs and ICIs. Associations between TKIs, ICIs and ONJ were assessed using reporting odds ratios (ROR), drug interaction signals based on the Ω shrinkage measure.
Results: 29 patients with ONJ events while on TKIs and ICIs were included in our case series. 240 were related to ONJ AEs. Specifically, 32.1% ICSRs were linked to sunitinib, 16.7% to lenvatinib, 12.9% to pazopanib, 12.5% to nivolumab, 10.0% to axitinib, 5.4% to sorafenib, 5.0% to pembrolizumab, 4.2% to cabozantinib, and 1.3% to ipilimumab. More ICSRs were generally seen in male and reported in Europe. The median age was 63 years. Renal cancer and lung cancer was the most common indication for TKIs and ICIs, respectively. Excluding missing data, the prevalence of mortality was highest for sunitinib-related ONJ ICSRs (18.5%), followed by sorafenib-related ONJ ICSRs (15.4%). With the criteria of ROR, sunitinib and lenvatinib were significantly associated with ONJ AEs. With the criteria of Ω, nivolumab + cabozantinib was significantly associated with ONJ AEs.
Conclusions: TKIs and ICIs have been reported to have significant ONJ side effects. Patients and physicians need to recognize and monitor these potentially fatal adverse events.
Complex Interplay of Sulfate Aerosols and Atmospheric Dynamics on Precipitation Vertical Structure and Latent Heat in the Beijing-Tianjin-Hebei Region: A Satellite Perspective
Authors: Li Rui , Zhu Hongxia , Yang Shuping , Zhao Hongwei , Wang Yu 
Published: April 2024

Abstract

An eight-year satellite observation dataset reveals that sulfate aerosols significantly influence the vertical structure of precipitation and latent heat (LH) in the Beijing-Tianjin-Hebei (BTH) region during summer. In this period, prevalent sulfate aerosols combine with warm, humid southerly winds and elevated convective available potential energy (CAPE), influencing precipitation dynamics. Under polluted conditions with specific CAPE and precipitation top temperature (PTT) ranges, precipitation particles experience accelerated growth within the mixed-phase layer, delineated by the -5°C to 2°C isotherms, compared to pristine environments. This results in a marked increase in both the intensity and height at which the maximum LH is released. Subsequent analysis reveals that hygroscopic sulfate aerosols, acting as cloud condensation nuclei (CCN), amplify the collision-coalescence process within the mixed layer amid high cloud water content, propelling rapid precipitation particle growth and elevating the PTT. This warming effect surpasses the cooling contribution from robust CAPE, culminating in a net elevation of PTT under polluted scenarios compared to pristine ones. Additionally, quantification of PTT sensitivity to both CAPE and aerosol optical depth (AOD) unveils a high consistency between satellite-detected PTT responses to CAPE and those predicted by cloud-resolving model simulations. The study deduces that the role of aerosols as CCN in either invigorating or diminishing the collision-coalescence process is contingent on the available cloud water.
Assessment of psychological distress in patients with cervical dysplasia according to age, education, information acquisition and information level
Authors: Scherer-Quenzer Anne Cathrine , Herbert Saskia Laureen , Schlaiss Tanja , Wöckel Achim , Diessner Joachim , Grunz Jan-Peter , Findeis Jelena , Kiesel Matthias 
Published: April 2024

Abstract

Purpose: This study aims to investigate the psychological distress experienced by patients with initial diagnosis of abnormal Pap smears or dysplastic changes of the cervix uteri. It is investigated whether patients' age, education, information level and approach to information acquisition have an impact on their psychological distress.
Methods: A total of 364 female patients, aged 20-80 years, referred to the special dysplasia consultation hour at the Department of Obstetrics and Gynecology, Wuerzburg, completed a questionnaire containing validated items to assess information level, information acquisition, information needs and psychological distress, including a distress thermometer. Data from questionnaires and medical reports were used for analysis.
Results: The study found that 56.9% of patients experienced psychological distress before their first visit. Patients under 44 years of age, especially those with concerns about fertility and sexuality, and those with lower levels of education showed higher levels of distress (p-value=0.018 and p-value=0.037). 40.9% of patients felt poorly informed and 53.7% of patients wanted more information before their visit. Correlational analysis showed that the method of obtaining information correlated with the desire for more information (p-value <0.001). Those who received information via the Internet felt less informed, wanted more information and experienced more anxiety.
Conclusion: These findings highlight the need for improved patient education strategies and effective doctor-patient communication to address the knowledge gap and reduce patient distress. In addition, healthcare providers should ensure that patients have access to reliable online resources for accurate information.
Exploring a quantitative formula for the total surgical amount in patients with type III acute acquired concomitant esotropia
Authors: Ren Xiaojun , Li Dongkan , Li Xian , Wang Pengjun , Chen Siyu , Huang Shiling , Jiang Naikun , Pan Meihua , He Huan , Chiang Yi-Chen 
Published: April 2024

Abstract

Background: To provide an amount-response reference for surgical planning in type III acute acquired concomitant esotropia (AACE).
Methods: Consecutive patients who underwent unilateral or bilateral medial rectus recession (MRrec) for AACE or common forms of esotropia (ET) correction from January 2019 to August 2023. All patients with a minimum follow-up of 3 months were analyzed. A pre- and post-surgical healthy eye behavior questionnaire was conducted on patients with AACE.
Results: In this study, we found that preoperative horizontal deviation had a significant effect on the total surgical amount of the patients. When predicting the total surgical amount of AACE patients, the recommended regression equation is Y = 3.259 + 0.174 preoperative horizontal deviation. Additionally, for AACE patients with preoperative horizontal deviation less than 40, the quantified reference formula for the total surgical amount was Y = 1.746 + 0.236 × preoperative horizontal deviation. For AACE patients with preoperative horizontal deviation greater than or equal to 40, the quantified reference formula for the total surgical amount was Y = 4.600 + 0.143 × preoperative horizontal deviation. The results of the questionnaire survey showed that the postoperative eye behaviors of AACE patients haveimproved.
Conclusions: This study conducted a detailed quantitative analysis of the impact of preoperative horizontal deviation levels on the total surgical amount for AACE patients, and established specific quantitative reference formulas to inform surgeons’ surgical planning. In addition, we suggest providing health education to patients after surgery, which can have a positive effect on their postoperative ocular behavior.
Nipple adenoma in prepubescent boy：a case report and review
Authors: Chen Jianzhe , Xia Tian , Chen Xiaomei , Yin Ansi , Xiong Jie , Duan Zhicai , Pan Guangrui , Zuo Huaiquan 
Published: April 2024

Abstract

Background：Nipple adenoma is a rare benign nipple tumor that is seldom seen in males and can be challenging to differentiate from Paget's disease.
Case report：We report the case of a boy with nipple adenoma who was initially misdiagnosed with Syringocystadenoma papilliferum combined with a tubular apocrine adenoma. Six months post-diagnosis, a nipple-areolar resection was conducted, followed by a purse-string suture technique to restore and reshape the nipple's appearance. Histopathological examination confirmed the diagnosis of nipple adenoma.
Conclusion：Nipple adenomas often prove difficult to alleviate without surgical intervention; surgical excision is deemed the gold standard for treatment. It is vital to establish a clear pathological diagnosis for nipple adenomas, as they are benign tumors, to prevent unnecessary overtreatment.
Antibacterial properties of mesoporous silica coated with cerium oxide nanoparticles in dental resin composite
Authors: Byun Sung-Yun , Han A Ruem , Kim Kwang-Mahn , Kwon Jae-Sung 
Published: April 2024

Abstract

Cerium oxide nanoparticles are known for their antibacterial effects resulting from Ce3+ to Ce4+ conversion. Application of such cerium oxide nanoparticles in dentistry has been previously considered but limited due to deterioration of mechanical properties. Hence, this study aimed to examine mesoporous silica (MCM-41) coated with cerium oxide nanoparticles and evaluate the antibacterial effects and mechanical properties when applied to dental composite resin. Cerium oxide nanoparticles were coated on the MCM-41 surface using the sol-gel method by adding cerium oxide nanoparticle precursor to the MCM-41 dispersion. The samples were tested for antibacterial activity against Streptococcus mutans via CFU and MTT assays. The mechanical properties were assessed by flexural strength and depth of cure according to ISO 4049. Data were analyzed using a t-test, one-way ANOVA, and Tukey’s post-hoc test (p = 0.05). The experimental group showed significantly increased antibacterial properties compared to the control groups (p < 0.005). The flexural strength exhibited a decreasing trend as the amount of cerium oxide nanoparticle-coated MCM-41 increased. However, the flexural strength and depth of cure values of the silane group met the ISO 4049 standard. Antibacterial properties increased with increasing amounts of cerium oxide nanoparticles. Although the mechanical properties decreased, silane treatment overcame this drawback. Hence, the cerium oxide nanoparticles coated on MCM-41 may be used for dental resin composite.