bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin‐4 inhibits proliferation of human leukemic megakaryoblast cell line mhh 225
Authors: Hassan H. T. , Grell S. , Borrmann‐Danso U. , Freund M. 
Published: February 2007

AbstractThe effects of six recombinant human cytokines: erythropoietin, GM‐CSF, G‐CSF, interleukin‐3, ‐4 and ‐6 on the proliferation and differentiation of a human multilineage myeloid leukemia cell line MHH 225, established from the bone marrow of an AML(M7) patient in our laboratory determined by changes in antigen expressions using monoclonal antibodies in APAAP technique were examined in liquid suspension culture. The MHH 225 cells have been growing exponentially without cytokines or conditioned media. About 90 per cent of MHH 225 cells are CD33+CD34+CD3–CD7–CD19–CD20–TdT– with 57.6 per cent, 28.3 per cent and 7.8 per cent of them being CD41+, glycophorin A+ and CD15+, respectively. After five days of treatment with erythropoietin, GM‐CSF, G‐CSF or IL‐6 no change was observed in MHH 225 cell antigens expression. IL‐3 (100 U/ml) induced a moderate increase in only CD13 and alpha naphthyl esterase positive cells from 6.5 ± 1.9 per cent and 5.7 ± 2.4 per cent in control cultures to 21.6 ± 3.0 per cent and 19.1 ± 2.8 per cent, respectively. On the other hand, 100 U/ml IL‐4 significantly increased the number of CD13, CD15 and alpha naphthyl esterase positive cells to 48.9 ± 5.0 per cent, 47.2 ± 3.6 per cent and 46.1 ± 3.0 per cent, p < 0.001, respectively. Also, 100 U/ml IL‐4 decreased the number of CD41 positive cells from 57.6 ± 2.8 per cent to only 25.9 ± 3.6 per cent and did not change the number of CD33 or glycophorin A positive cells. The present results showed that out of the six myelopoietic growth factors tested, IL‐4 was the only one to inhibit selectively the proliferation of CD33+CD41+ leukemic megakaryoblast cells suggesting that IL‐4 may have a lineage regulatory effect in favour of a myeloblastic CD33+CD13+CD15+ at the expense of a megakaryoblastic CD33+CD41+ amplification in human leukemia cells and with apparently no effect on leukemic erythroblast cells. The MHH 225 cell line provides a useful tool and freely available model to scientists for studying signal transduction via IL‐4 and for studies of‘lineage switch’.
Autologous bone marrow transplantation in adults with non‐Hodgkin's lymphoma: A southwest oncology group study
Authors: Saez Ruben , Dahlberg Steve , Appelbaum Frederick R. , Hartsock Robert J. , Lemaistre Frederick , Coltman Charles A. , Fisher Richard I. 
Published: February 2007

AbstractPatients with non‐Hodgkin's lymphoma (NHL) who fail conventional chemotherapy have a dismal outcome. Reports from single institutions utilizing high‐dose chemoradiotherapy plus Autologous Bone Marrow Transplantation (ABMT) in this setting suggest three‐year disease‐free survival between 15–60 per cent. From 1985 to 1989 the Southwest Oncology Group performed a prospective multi‐institutional study involving ABMT in relapsed/refractory NHL.Forty‐five patients, ages 6–60 (median 38), with relapsed NHL were treated with high‐dose cyclophosphamide (60 mg/kg/d × 2), total body irradiation (200 cGy/d × 6), and autologous unpurged bone marrow. Histologic subtypes included high grade lymphoma (10), intermediate grade lymphoma (33), and low grade lymphoma (2). Disease status pre‐ABMT was sensitive relapse (16), resistant relapse (13), and untreated relapse (16).The actuarial three‐year event‐free survival and overall survival for all patients were 27 per cent and 38 per cent respectively. Causes of failure included regimen‐related deaths (4), lack of response (10), or tumour progression (20) which occurred at a median of 5 months (1–22) post‐ABMT and usually at previous sites of involvement. Response to salvage therapy pre‐ABMT, a reflection of a tumour's biological behaviour, was the most important predictor of good outcome post‐ABMT.This study confirms that a significant number of patients with recurrent NHL can achieve prolonged disease‐free survival after ABMT.
Induction of eosinophilic granules, nonspecific esterase activity and CD14 expression in the human eosinophilic leukemia cell line, EoL‐1
Authors: Shintaku Noriaki , Ohshima Yusei , Jung Eun‐Young , Kanazashi Shu‐Ichi , Sumimoto Shin‐Ichi , Ohmori Katsuyuki , Heike Toshio , Katamura Kenji , Mayumi Mitsufumi 
Published: February 2007

AbstractWe examined the expression of eosinophilic granules, esterase activity and CD14 in a human eosinophilic cell line, EoL‐1. Unstimulated EoL‐1 cells were weakly positive for nonspecific esterase, but negative for surface CD14, and contained a few eosinophilic granule‐positive cells. A combination of G‐CSF and TNF‐α increased the eosinophilic granule‐containing cells, but failed to increase esterase activity or CD14 expression. IFN‐γ alone or in combination with TNF‐α enhanced nonspecific esterase activity but failed to induce CD14 expression or increase eosinophilic granule‐containing cells. dbcAMP increased eosinophilic granule‐containing cells, nonspecific esterase activity and CD14 expression. Specific esterase activity was not detected in any circumstances. EoL‐1 cells fractionated by density gradients or CD14 expression showed nonspecific esterase activity and CD14 expression in both the eosinophilic granule‐positive and negative cell populations. Forskolin and butyrate had a synergistic effect on CD14 induction and protein kinase A was suggested to play a role in dbcAMP‐induced CD14 expression. A protein kinase C activator, phorbol 12‐myristate 13‐acetate, did not increase eosinophilic granules, nonspecific esterase activity or CD14 experession in EoL‐1 cells. The results show that EoL‐1 cells can express nonspecific esterase and CD14, but the expression is not necessarily restricted to cells which have differentiated into the monocyte/macrophage lineage.
Differential platelet‐associated factor gene expression by a panel of human cell lines with megakaryoblastic properties after treatment with phorbol myristate acetate
Authors: Micallef Mark , Matsuo Yoshinobu , Takayama Kazue , Ariyasu Toshio , Otani Takeshi , Minowada Jun 
Published: February 2007

AbstractFour human megakaryocytoid cell lines, namely MOLM‐1, MOLM‐7, MEG‐01 and HEL, were treated with phorbol 12‐myristate 13‐acetate (PMA) and expression of the platelet‐derived growth factor (PDGF) A chain, von Willebrand factor (vWF) and endothelial cell growth factor (ECGF) genes was examined by the reverse transcriptase‐polymerase chain reaction (RT‐PCR) using specific primers. The gene for PDGF A chain is constitutively weakly expressed by MEG‐01 cells and strong expression is induced in MEG‐01, MOLM‐1 and MOLM‐7 but not in HEL cells after treatment with PMA for three days. All four cell lines express the vWF gene both constitutively and after exposure to PMA. None of the cell lines constitutively express the gene for ECGF but MEG‐01 cells can be induced to do so after treatment with the phorbol diester. Immunohistochemical staining after exposure to PMA showed that the expression of the platelet‐associated markers CD41 and CD61 was enhanced in all cell lines indicating possible differentiation along the megakaryocyte lineage. Our results illustrate differential platelet‐associated factor gene expression in different megakaryoblastic cell populations in response to treatment with PMA, and suggest that expressions of the PDGF A chain gene and the ECGF gene may be good markers for megakaryocyte maturation.
Interleukin‐10 production by a B‐cell line derived from human post‐transplant lymphoproliferative disease
Authors: Randhawa Parmjeet , Nalesnik Michael , Demetris Jake , Zeevi Adriana 
Published: February 2007

AbstractInterleukin‐10 (IL‐10) is a cytokine known to regulate growth and differentiation in activated human B cells. We studied IL‐10 production in a B‐cell line derived from Epstein‐Barr virus associated post‐transplant lymphoproliferative disease (PTLD). Reverse transcriptase polymerase chain reaction (RTPCR) demonstrated IL‐10 mRNA within the cells. Transcripts of the virally encoded homologue BCRF‐1 were not detected. ELISA assays demonstrated translation of IL‐10 message into the corresponding cytokine, and its subsequent secretion into the culture medium. The rate of 3H‐thymidine incorporation by the PTLD cells was not affected by immunologic neutralization of the secreted cytokine, or, by addition of exogenous recombinant IL‐10 to the culture medium. Thus, IL‐10 does not have an autocrine growth regulatory role in this PTLD line.
IL6 and IL6 receptor expression in Burkitt's lymphoma and lymphoblastoid cell lines: Promotion of IL6 receptor expression by EBV
Authors: Klein S. C. , Jücker M. , Abts H. , Tesch H. 
Published: February 2007

AbstractWe have analysed a panel of different Burkitt's lymphoma (BL) and lymphoblastoid cell lines (LCLs) for the expression of IL6 and IL6 receptor (IL6R). Epstein‐Barr‐Virus (EBV) positive or negative BL cell lines and the corresponding lymphoblastoid cell lines (LCL), derived from EBV immortalized mononuclear cells of the BL patients, were tested for the expression of IL6 mRNA and protein by Northern blot experiments and ELISA, and for the expression of the IL6R mRNA and protein by Northern blot Reverse Transcriptase‐Polymerase Chain Reaction (RT‐PCR) and flow cytometry. Our results demonstrate that six out of 19 Burkitt's lymphoma cell lines produced IL6 constitutively. All three cell lines infected with the EBV substrain B95‐8 (B95‐8 convertants) produced IL6, in contrast to the original EBV negative lines and to the cell lines infected with the EBV substrain P3HR1 (P3HR1 convertants). The produced IL6 was biologically active as shown by proliferation of the IL6 dependent cell line TEPC 1033 C2. The two BL cell lines with the highest level of IL6 production (190 pg/ml and 550 pg/ml) expressed in addition IL6R molecules on the cell surface. Monoclonal antibodies directed against IL6 did not inhibit the growth of these two BL cell lines, thus excluding autocrine stimulation in these lines. IL6R expression could be further demonstrated in all LCLs analysed, in five out of seven EBV positive BLs and two out of three B95‐8 convertants, but only in one out of the six EBV negative BL cell lines. Our results suggest that EBV in immortalized B cells and in Burkitt's lymphoma cells can promote IL6 receptor expression.
Prognostic factors for primary gastrointestinal lymphoma
Authors: Liang Raymond , Todd David , Chan T. K. , Chiu Edmond , Lie Albert , Kwong Yok‐Lam , Choy Damon , Ho Faith C. S. 
Published: February 2007

AbstractThe gastrointestinal tract is a common primary extranodal site for non‐Hodgkin's lymphoma. There is however no uniform consensus on its pathological classification, clinical staging system and management. This paper reports the experience in the management of 425 Chinese patients with primary gastrointestinal lymphoma in Hong Kong from January 1975 to June 1993. There were 230 (54 per cent) males and 195 (46 per cent) females. Their median age was 53 years. The primary sites were: the esophagus in three (1 per cent), stomach in 238 (56 per cent), small intestine in 131 (31 per cent) and large intestine in 53 (12 per cent). According to the Working Formulation, there were 20 (4.7 per cent) small lymphocytic, 10 (2.4 per cent) follicular small cleaved cell, 15 (3.5 per cent) follicular mixed, five (1.2 per cent) follicular large cell, 40 (9.4 per cent) diffuse small cleaved cell, 50 (12 per cent) diffuse mixed, 181 (43 per cent) diffuse large cell, 30 (7.1 per cent) immunoblastic, five (1.2 per cent) lymphoblastic, 10 (2.4 per cent) diffuse small non‐cleaved cell and 50 (14 per cent) unclassifiable lymphoma. Immunophenotyping was performed in 199 (47 per cent) patients: 90 per cent B‐cell, 7 per cent T‐cell and 3 per cent uncertain. According to a Manchester system, 81 (19 per cent) patients had stage I disease, 44 (10 per cent) stage II, 85 (20 per cent) stage III and 215 (51 per cent) stage IV. B symptoms were present in 275 (65 per cent) patients and bulky disease in 104 (25 per cent). Surgery followed by chemotherapy was the mainstaly of treatment. Of the 408 patients treated, 63 per cent had a complete remission with relapse rate of 42 per cent. For those with complete remission, 47 per cent were free from disease at 5 years. The overall median survival of all patients was 45 per cent at 5 years. Multivariate analysis revealed that significant independent prognostic factors predicting better survival were young age of <60 years, low grade histology, stage I and II disease and absence of bulky tumour. For gastric lymphoma, aggressive surgery did not significantly improve their outcome. Chemotherapy appears to play an important role in the management of gastrointestinal lymphoma. Better classification of the primary gastrointestinal lymphoma and more refined stratification of the patients according to the prognostic variables my allow individualization of treatment. Prospective randomized studies are essential to define the relative roles of surgery, chemotherapy and radiotherapy.
Identification of chromosomal rearrangements in the human myeloid leukemia cell line GF‐D8 by dual‐colour fluorescence in situ hybridization
Authors: Doneda Luisa , Biondi Andrea , Rambaldi Alessandro , Larizza Lidia 
Published: February 2007

AbstractFluorescence In Situ Hybridization (FISH) studies with chromosome‐specific libraries and repetitive probes were performed on the human acute myeloid leukemia cell line GF‐D8 in order to define the complex chromosomal rearrangements observed by conventional cytogenetic analysis. Two‐colour FISH with whole chromosome painting probes 8 and 11 showed that the add(8) chromosome had an 11‐derived region inserted at q24, whereas the add(11) chromosome had an 8‐derived region translocated onto q23. It also demonstrated that no normal chromosome 11 is present in GF‐D8 cells, since a translocation involving chromosomes 11 and 17q was detected in addition to the add(11). The der(7) chromosome with extra material in its long arm, identified by QFQ and GTG banding, turned out to have a chromosome 15‐derived segment translocated to q22. The deletion of 7q was proved to be interstitial, as the 7q‐specific telomere as well as a tiny 7‐specific band were observed on an unknown chromosome. Fine mapping of the breakpoints involved in the multiple chromosomal rearrangements of the GF‐D8 cell line might provide insights into the mechanisms of myeloid leukaemogenesis.
Cell kinetic analysis of non‐Hodgkin's lymphomas using in vivo iododeoxyuridine incorporation and flow cytometry
Authors: Erlanson Martin , Lindh Jack , Zackrisson Björn , Landberg Göran , Roos Göran 
Published: February 2007

AbstractThe aim of this study was to analyse dynamic cell proliferation parameters in non‐Hodgkin's lymphomas. Sixty‐one patients with newly diagnosed or with recurrent disease were given iododeoxyuridine (IdUrd) intravenously near 4 h prior to tumour biopsy. After staining with an IdUrd reactive antibody and propidium iodide, S‐phase fraction (SPF), labelling index (LI), S‐phase duration time (Ts) and potential tumour doubling time (Tpot) were determined by flow cytometry. Thirty‐eight samples, 15 low grade (LGM) and 23 high grade (HGM) malignant lymphomas, were possible to evaluate. Twenty‐three cases were excluded due to aneuploidy, insufficient amount of material or technical problems.Tpot values varied between 0.8–32.9 days (mean 7.0 days). HGM lymphomas had shorter mean Tpot times than LGM lymphomas (4.8 versus 10.4 days, p=0.05). For Ts the range was 4.2–20.1 h (mean 9.1 h), and a difference between the two histological groups was demonstrated with a longer mean Ts for HGM compared with LGM cases (10.0 versus 7.8 h, p=0.04). Tpot showed a negative correlation with SPF (P=0.003), and Ts demonstrated a positive correlation to SPF (p=0.02). The clinical significance of the dynamic cell proliferation parameters studied remains to be clarified, but the interelationships between Ts/SPF and Ts/morphologic subtype might be factors of interest for future prognostic studies in malignant lymphomas.
Temperature drop analysis of the thruster in a space cryogenic environment
Authors: Xiao Ze‐Juan , Cheng Hui‐Er , Zhou Hong‐Ling , Huang Rui‐Sheng 
Published: February 2007

AbstractBased on the conservation of energy, a coupling heat‐transfer physical model and a set of mathematical equations are put forward to calculate the main components of the thruster, including the capillary injection tube, the aggregate organ, the injection plate, and the bracket when they are exposed to a space cryogenic environment. The typical temperature drop course of a 10N monopropellant thruster has been calculated by this computational model. The calculation results agree well with test data in a vacuum cryogenic simulation experiment performed on the ground. The temperature of the injection tube provides the thermal boundary conditions for the propellant temperature drop calculation while flowing through it. This provided the criterion to judge whether the propellant freezes or not. The upper stage has no air conditioning, so the injection tube is a weak link for the cryogenic reliability work of the thruster. This is considered one of the most important areas of the whole for reliability research. © 2007 Wiley Periodicals, Inc. Heat Trans Asian Res, 36(2): 85–95, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20144
The impact of methamphetamine use on subjective well‐being in an Internet survey: preliminary findings
Authors: Looby Alison , Earleywine Mitch 
Published: February 2007

AbstractMethamphetamine is one of the most widely used stimulants worldwide. Common reasons for use of the drug include efforts to improve or enhance one's life and to uplift one's mood. Nevertheless, acute effects of the drug lead to temporary improvements in mood followed by negative affect. The purpose of the present study was to expand on the current literature and examine other aspects of mood and satisfaction with life in methamphetamine users. Over 6000 adults completed an Internet survey and reported on depression, apathy, satisfaction with life, happiness, and subjective well‐being, in addition to measures of methamphetamine use. We compared those who had used methamphetamine at least once within the past year (N = 610) to those who had never used (N = 6063). Methamphetamine use accounted for significant variance in depression, apathy, satisfaction with life, happiness, and subjective well‐being even when alcohol and other drugs served as covariates. Methamphetamine use may decrease one's subjective well‐being instead of enhancing it, which is contradictory to the perceptions of many users. Increasing awareness about methamphetamine's negative impact on mood and life satisfaction might help decrease prevalence of the drug's use and associated troubles. Copyright © 2007 John Wiley & Sons, Ltd.
Biologic characterization of gross lymphoma homogenates concentrated by density gradient and differential centrifugation
Authors: Wahren Britta 
Published: February 2007

AbstractFractions of Gross lymphoma homogenates were prepared by density gradient centrifugation or by differential centrifugation. Primary lymphomas developed in 80% of newborn mice inoculated with pellets obtained after differential centrifugation and in 48% of mice inoculated with fractions of Gross lymphoma homogenate with a density of 1.16–1.18. Such fractions induced a relative transplantation resistance as measured by a decrease in the frequency of tumor takes and a prolonged latency period when isogenic Gross lymphoma cells were inoculated into immunized mice.Sera obtained from mice immunized with fractions of density 1.16–1.18 from Gross lymphoma homogenate were shown to contain cytotoxic antibodies active against Gross lymphoma cells. The results indicate that viral particles are responsible for the induction of antibodies against the Gross lymphoma antigen, either directly by sharing antigenic properties with the surface of the lymphoma cells from which they are derived, or indirectly by infection and transformation of susceptible cells.
The covalent binding of metabolites of dimethylaminoazobenzene, β‐naphthylamine and aniline to nucleic acids in vivo
Authors: Roberts J. J. , Warwick G. P. 
Published: February 2007

Abstract
Following injection of dimethylaminoazobenzene‐3H labelled in the primed ring (150 mg/kg) into male albino rats and guinea‐pigs maintained on a diet containing 10% protein, radioactivity was covalently bound to r‐RNA, DNA, cytoplasmic protein and nuclear protein of liver and spleen.
For liver the maximum levels of binding (μM/g × 102) were r‐RNA 11.4, cytoplasmic protein 54, nuclear protein 39, DNA 1.4; for spleen: r‐RNA 1.0, DNA 0.5, cytoplasmic protein 89, and nuclear protein 39; and for guinea‐pig liver r‐RNA 3.4, DNA 2.2, cytoplasmic protein 55, nuclear protein 28, indicating a much higher ratio of protein binding to r‐RNA binding than in the rat (16 compared with 5). The difference in ratio was also large for the binding to two transplanted hepatomas in August and Hooded rats compared with albino rat liver (37 and 13).
For rat liver r‐RNA and protein the decay of radioactivity paralleled the rate of turnover of r‐RNA (as measured by orotic acid‐3H incorporation) and of protein. The level of DNA‐bound radioactivity remained constant for 290 hours.
The radioactivity associated with the nucleic acids and proteins remained bound after treatment with 0.3 N KOH which converts the nucleic acids to nucleotides, and with Crotalus adamanteus venom which converts them to purine and pyrimidine nucleosides. Treatment with N HCl for 1 hr at 100°C which forms purine bases and pyrimidine nucleotides and degrades proteins into peptides and amino acids, liberated the majority of the radioactivity so that it became extractable at various pH values.
The binding of β‐naphthylamine‐3H and aniline‐3H hydrochloride to rat liver, spleen and kidney were also studied. Binding occurred to DNA, ribosomal RNA and protein in each case. For β‐naphthylamine the levels of metabolite binding were similar to dimethylaminoazobenzene metabolite binding to guinea‐pig liver components. For aniline hydrochloride, binding to ribosomal RNA and protein was of a low order.
The possible relevance of these findings to the problem of carcinogenesis is discussed.
Anahormones of growth hormone, exophthalmic factor, melanophore‐stimulating hormone, adrenocorticotropin and gonadotropin in experimental and clinical endocrinology
Authors: Dilman V. M. 
Published: February 2007

AbstractData on the dissociation of some properties of protein hormones are summarized. Preparations have been made in which the hormonal effect has been lost whereas the antigenic specificity or ability to localize in the target tissue has been retained.Hormone derivatives with these properties have been defined as “anahormones” by analogy with anatoxins. The anahormones can inhibit the activity of endogenous hormones.The ability of modified hormones to inhibit hormone action was retained in the following inactivated hormones: human acetylated somatotropin, acetylated exophthalmic factor, and MSH and ACTH (melanophore activity) after treatment with Lithospermum officinale extract.The retention of antigenic specificity was observed in the following inactivated hormones: iodinated chorionic gonadotropin, acetylated human somatotropin, acetylated thyrotropin and formalinized sheep gonadotropin.It was also shown that immunization by gonadotropins inhibited to a considerable extent the growth of intrasplenic transplants of the ovary in rats.The possibility of using anahormones to counteract the carcinogenic influence of an excess of endogenous hormones is discussed.