bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgenic Dendra2::tau expression allows in vivo monitoring of tau proteostasis in Caenorhabditis elegans
Authors: Han Marina , Saxton Aleen , Currey Heather , Waldherr Sarah M. , Liachko Nicole F. , Kraemer Brian C. 
Published: March 2024

ABSTRACT
Protein homeostasis is perturbed in aging-related neurodegenerative diseases called tauopathies, which are pathologically characterized by aggregation of the microtubule-associated protein tau (encoded by the human MAPT gene). Transgenic Caenorhabditis elegans serve as a powerful model organism to study tauopathy disease mechanisms, but moderating transgenic expression level has proven problematic. To study neuronal tau proteostasis, we generated a suite of transgenic strains expressing low, medium or high levels of Dendra2::tau fusion proteins by comparing integrated multicopy transgene arrays with single-copy safe-harbor locus strains generated by recombinase-mediated cassette exchange. Multicopy Dendra2::tau strains exhibited expression level-dependent neuronal dysfunction that was modifiable by known genetic suppressors or an enhancer of tauopathy. Single-copy Dendra2::tau strains lacked distinguishable phenotypes on their own but enabled detection of enhancer-driven neuronal dysfunction. We used multicopy Dendra2::tau strains in optical pulse-chase experiments measuring tau turnover in vivo and found that Dendra2::tau turned over faster than the relatively stable Dendra2. Furthermore, Dendra2::tau turnover was dependent on the protein expression level and independent of co-expression with human TDP-43 (officially known as TARDBP), an aggregating protein interacting with pathological tau. We present Dendra2::tau transgenic C. elegans as a novel tool for investigating molecular mechanisms of tau proteostasis.
Sentiment analysis of Indonesian tweets on COVID-19 and COVID-19 vaccinations
Authors: Kalanjati Viskasari Pintoko , Hasanatuludhhiyah Nurina , d'Arqom Annette , Arsyi Danial H. , Marchianti Ancah Caesarina Novi , Muhammad Azlin , Purwitasari Diana 
Published: March 2024

Background Sentiments and opinions regarding COVID-19 and the COVID-19 vaccination on Indonesian-language Twitter are scarcely reported in one comprehensive study, and thus were aimed at our study. We also analyzed fake news and facts, and Twitter engagement to understand people’s perceptions and beliefs that determine public health literacy. Methods We collected 3,489,367 tweets data from January 2020 to August 2021. We analyzed factual and fake news using the string comparison method. The difflib library was used to measure similarity. The user’s engagement was analyzed by averaging the engagement metrics of tweets, retweets, favorites, replies, and posts shared with sentiments and opinions regarding COVID-19 and COVID-19 vaccination. Result Positive sentiments on COVID-19 and COVID-19 vaccination dominated, however, the negative sentiments increased during the beginning of the implementation of restrictions on community activities (PPKM). The tweets were dominated by the importance of health protocols (washing hands, keeping distance, and wearing masks). Several types of vaccines were on top of the word count in the vaccine subtopic. Acceptance of the vaccination increased during the studied period, and the fake news was overweighed by the facts. The tweets were dynamic and showed that the engaged topics were changed from the nature of COVID-19 to the vaccination and virus mutation which peaked in the early and middle terms of 2021. The public sentiment and engagement were shifted from hesitancy to anxiety towards the safety and effectiveness of the vaccines, whilst changed again into wariness on an uprising of the delta variant. Conclusion Understanding public sentiment and opinion can help policymakers to plan the best strategy to cope with the pandemic. Positive sentiments and fact-based opinions on COVID-19, and COVID-19 vaccination had been shown predominantly. However, sufficient health literacy levels could yet be predicted and sought for further study.
Field study of parasitic contamination of fruits, vegetables and leafy greens in the Ecuadorian Andes
Authors: González-Ramírez Luisa Carolina , Djabayan-Djibeyan Pablo , Prato José G. , García Ríos Cecilia Alejandra , Carrero Julio César , Trelis María , Fuentes Màrius Vicent 
Published: March 2024

Background: Raw vegetables have been considered vehicles of enteroparasites. South American countries are among the most important exporters of fresh vegetables, including Ecuador, which has a tropical climate and soils rich in organic matter that allow it to harvest throughout the year for sale to different countries. The aim of the study was to assess the occurrence of the parasitic contamination of fruits, vegetables and leafy greens grown in an agricultural area of the Ecuadorian Andes. Methods: A cross-sectional field study was conducted with snowball sampling on 1,416 samples (516 fruits, 488 vegetables, and 412 leafy greens). Each sample were washed with water, and the resulting solution after removing the vegetables, was subjected to 24-hour sedimentation. The concentrated sediment underwent microscopic analysis. Results: The overall positivity for parasitic contamination was 63.4%, with leafy greens having the highest contamination rate (76.9%) (P<0.0001), surpassing vegetables (67.8%) and fruits (48.4%). Cabbage (100%), onions (84%), and strawberries (60.2%) emerged as the most contaminated within their respective groups. Protozoa were more prevalent (49.6%) than helminths (15.5%) (P<0.0001). Blastocystis sp. (33.5%) ranked highest, followed by Eimeria spp. (26.3%), Entamoeba spp. (10.3%), Giardia spp. (8.3%), Balantidium spp. (6.9%), Cryptosporidium spp. (6.6%), Cyclospora spp. (4.4%), Cystoisospora spp. (0.5%), Strongylida (15.5%), and Ascaris spp. (0.4%). Conclusion: The study reveals that vegetables and fruits for human consumption from this area of the Ecuadorian Andes are highly contaminated with various parasites, constituting a possible source of infection for humans and animals in this area, or in non-endemic areas where these products are marketed. The finding emphasizes the need for strict hygienic measures in agricultural crops, which will be properly achieved through the treatment of soil, manure and water used for cultivation.
Small and Medium Enterprises Amidst the Pandemic and Reopening: Digital Edge and Transformation
Authors: Cong Lin William , Yang Xiaohan , Zhang Xiaobo 
Published: March 2024

Using administrative universal business registration data as well as primary offline and online surveys of small businesses (including unregistered self-employments) in China, we examine (i) whether digitization helps small and medium enterprises (SMEs) better cope with the COVID-19 pandemic and (ii) whether the pandemic has spurred digital technology adoption. We document significant economic benefits of digitization in increasing SMEs’ resilience against such a large shock, as seen through mitigated demand decline, sustainable cash flow, ability to quickly reopen, and positive outlook for growth. After the January 2020 lockdown, firm entries exhibited a V-shaped pattern, with entries of e-commerce firms experiencing a less pronounced immediate drop and a quicker rebound. Moreover, the pandemic has accelerated the digital transformation of existing firms and the industry in multiple dimensions (e.g., altering operation scope to include e-commerce, allowing remote work, and adopting electronic information systems). The effect persists more than one year after reopening, and it is more pronounced for certain sectors, firms in industrial clusters, and areas with more digital inclusion but less financial efficiency, constituting initial evidence for the long-term impact of the pandemic and the supposedly transitory mitigation policies. This paper was accepted by David Simchi-Levi, finance. Funding: This research was funded in part by the China Natural Science Foundation [Grants 71874008, 71441008, 71873121, and 72192844], Peking University, the Kauffman Foundation [Junior Fellowship], and the FinTech Chair at Paris–Dauphine University–Université Paris Sciences et Lettres. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2023.02424 .
Motivated Belief Updating and Rationalization of Information
Authors: Drobner Christoph , Goerg Sebastian J. 
Published: March 2024

We study belief updating about relative performance in an ego-relevant task. Manipulating the perceived ego relevance of the task, we show that subjects substantially overweight positive information relative to negative information because they derive direct utility from holding positive beliefs. This finding provides a behavioral explanation why and how overconfidence can evolve in the presence of objective information. Moreover, we document that subjects who receive more negative information downplay the ego relevance of the task. These findings suggest that subjects use two alternative strategies to protect their ego when presented with objective information. This paper was accepted by Marie Claire Villeval, behavioral economics and decision analysis. Funding: The authors gratefully acknowledge financial support from the ExperimenTUM. Supplemental Material: The online appendices and data files are available at https://doi.org/10.1287/mnsc.2023.02537 .
Marginal Values of a Stochastic Game
Authors: Attia Luc , Oliu-Barton Miquel , Saona Raimundo 
Published: March 2024

Zero-sum stochastic games are parameterized by payoffs, transitions, and possibly a discount rate. In this article, we study how the main solution concepts, the discounted and undiscounted values, vary when these parameters are perturbed. We focus on the marginal values, introduced by Mills in 1956 in the context of matrix games—that is, the directional derivatives of the value along any fixed perturbation. We provide a formula for the marginal values of a discounted stochastic game. Further, under mild assumptions on the perturbation, we provide a formula for their limit as the discount rate vanishes and for the marginal values of an undiscounted stochastic game. We also show, via an example, that the two latter differ in general. Funding: This work was supported by Fondation CFM pour la Recherche; the European Research Council [Grant ERC-CoG-863818 (ForM-SMArt)]; and Agence Nationale de la Recherche [Grant ANR-21-CE40-0020].
Cross-Site and Cross-Generation Knowledge Transfer in High-Tech After-Sales Service
Authors: Zijlstra F. S. , Alblas A. A. , Langerak F. 
Published: March 2024

Learning and knowledge transfer are crucial to organizational success. Knowledge can transfer between sites and generations, yet prior research has studied both types of knowledge transfer in isolation. Understanding their combined effect is essential because many manufacturing and service organizations have multiple sites and generations. In contrast to prior research, we study both types of knowledge transfer simultaneously in a high-tech after-sales service organization. In doing so, we provide new insights into the learning processes of high-tech firms providing global after-service. We leverage 10 years of weekly observations of after-sales service experience and performance of a firm in the semiconductor industry to test learning curve models with cross-site and cross-generation knowledge transfer and temporal distance between generations. Our empirical model tests the impact of these different sources of experience on the downtime performance of five machine generations serviced by 30 globally distributed service sites. We find knowledge transfer across sites and generations, except for cross-site knowledge transfer from newer to older generations. That is, the introduction of new products disturbs the cross-site learning process of older products. Furthermore, cross-generation knowledge transfer is conditional to the temporal distance between product generations. Knowledge transfer decreases when the temporal distance increases. Funding: This research was funded by the high-tech company that facilitated this study.
Enhancing the spatial resolution of time-of-flight based non-line-of-sight imaging via instrument response function deconvolution
Authors: Wang DingJie , Hao Wei , Tian YuYuan , Xu WeiHao , Tian Yuan , Cheng HaiHao , Chen SongMao , Zhang Ning , Zhu WenHua , Su XiuQin 
Published: March 2024

Non-line-of-sight (NLOS) imaging retrieves the hidden scenes by utilizing the signals indirectly reflected by the relay wall. Benefiting from the picosecond-level timing accuracy, time-correlated single photon counting (TCSPC) based NLOS imaging can achieve theoretical spatial resolutions up to millimeter level. However, in practical applications, the total temporal resolution (also known as total time jitter, TTJ) of most current TCSPC systems exceeds hundreds of picoseconds due to the combined effects of multiple electronic devices, which restricts the underlying spatial resolution of NLOS imaging. In this paper, an instrument response function deconvolution (IRF-DC) method is proposed to overcome the constraints of a TCSPC system’s TTJ on the spatial resolution of NLOS imaging. Specifically, we model the transient measurements as Poisson convolution process with the normalized IRF as convolution kernel, and solve the inverse problem with iterative deconvolution algorithm, which significantly improves the spatial resolution of NLOS imaging after reconstruction. Numerical simulations show that the IRF-DC facilitates light-cone transform and frequency-wavenumber migration solver to achieve successful reconstruction even when the system’s TTJ reaches 1200 ps, which is equivalent to what was previously possible when TTJ was about 200 ps. In addition, the IRF-DC produces satisfactory reconstruction outcomes when the signal-to-noise ratio (SNR) is low. Furthermore, the effectiveness of the proposed method has also been experimentally verified. The proposed IRF-DC method is highly applicable and efficient, which may promote the development of high-resolution NLOS imaging.
Factors related to willingness to participate in biomedical research on neglected tropical diseases: A systematic review
Authors: Raimundo-Silva Vinícius , Marques Caio Torres , Fonseca João Rezende , Martínez-Silveira Martha Silvia , Reis Mitermayer Galvão 
Published: March 2024

Background
Understanding the barriers to and facilitators of participation in research could enhance recruitment rates for biomedical research on Neglected Tropical Diseases (NTDs) and help to avoid the problems associated with poor recruitment.

Methodology/principal findings
We conducted a systematic review to identify factors related to willingness to participate in biomedical research on Neglected Tropical Diseases (NTDs). Our search included the following databases: Medline/PubMed, Embase (Embase.com), Global Index Medicus (WHO), Web of Science (Core collection), and gray literature. We included studies that analyzed or reported factors associated with willingness to participate in NTD research, using either quantitative methods (such as clinical trials, cohorts, and cross-sectional studies) or qualitative methods (such as focus group discussions, semi-structured interviews, and in-depth interviews). There were no language restrictions, but we excluded review articles, notes, case reports, letters to the editor, editor’s notes, extended abstracts, proceedings, patents, editorials, and other editorial materials.
Screening of citations, data extraction, and risk of bias assessment was conducted by independent reviewers, according to the study protocol registered on PROSPERO. For analyses, we assessed the frequency of barriers, enablers, and the frequency of recruitment interventions mentioned in the included studies. The protocol for this systematic review was registered under registration number CRD42020212536. (S1 Appendix)
We identified 2070 citations, 1470 from the databases, and 600 from other sources. From those, eleven studies were selected for data extraction and analysis. The studies were conducted in Africa, Asia, and North America. Personal health benefits, monetary benefits, and community engagement and sensitization strategies were identified as the main reasons for participating in biomedical research on Neglected Tropical Diseases (NTDs). However, distrust in researchers, lack of knowledge about research methods among potential participants, and previous negative experiences were identified as the main barriers to participating in biomedical research on NTDs.

Conclusions/significance
This systematic review provides recommendations for improving adherence to biomedical research on Neglected Tropical Diseases, which can be applied in practice.
Influence of acclimatization time on parameters of barometric whole-body plethysmography in healthy adult cats
Authors: Benz Petra , Zablotski Yury , Schulz Bianka 
Published: March 2024

Background
Pulmonary function testing by barometric whole-body plethysmography (BWBP) is a long-established and well-accepted, non-invasive investigative procedure in cats.

Hypothesis/Objectives
To evaluate, if different acclimatization times influence the measurement parameters of BWBP in healthy adult cats.

Animals
48 healthy adult cats.

Methods
In the prospective observational study, healthy cats were placed in a measuring chamber and BWBP was performed over 30 minutes. Parameters obtained during the three measurement units of 10 minutes each (T1, T2 and T3) were compared.

Results
All measurement parameters except for tidal volume per body weight changed significantly (p<0.05) over the three time periods. From T1-T2, the parameters minute volume per body weight (p<0.001), peak inspiratory flow per body weight (p<0.001), peak expiratory flow per body weight (p = 0.002), pause (p = 0.03), enhanced pause (p = 0.03) and quotient of peak expiratory flow divided by expiratory flow at end expiratory volume plus 50% tidal volume (p = 0.03) changed significantly. From the time interval T2-T3, only respiratory rate (p = 0.02), inspiratory time (p = 0.02), expiratory time (p = 0.04), and relaxation time (p = 0.01) changed significantly. All measurement parameters except for tidal volume per body weight changed significantly (p<0.05) between T1 and T3. Age had a significant influence on all parameters except for peak expiratory flow per body weight and peak inspiratory flow per body weight. The parameters were not influenced by sex.

Conclusion and clinical importance
All measurement parameters except tidal volume per body weight were significantly affected by acclimatization time. Controlling for age and sex, there was still a significant influence of acclimatization time on all parameters except for tidal volume per body weight. Standardization of the acclimatization time for future studies would be appropriate in order to maintain comparability.
Systematic Literature Review of Disseminating Health Information to BIPOC Communities
Authors: Kelly Damien , Ostovar-Kermani Tiffany G 
Published: March 2024

This study conducted a systematic literature review to explore best practices for disseminating health information and strategies to advance equitable health outcomes in Black, Indigenous, and People of Color (BIPOC) communities. The researchers searched databases such as Science.gov, ERIC, Social Science Research Network, and CHAT AI, identifying 4,726 articles. After quality evaluation, 23 articles were included in the review, encompassing qualitative, quantitative, and mixed methods studies conducted between January 1, 2005, and December 31, 2023.
The most effective health information dissemination methods involved direct community interactions, utilizing versatile communication strategies. Real-time engagement and accessibility to public health experts enhanced community trust and participation. Narrative storytelling, focus groups, workshops, and technology for visual representations were effective tools. However, obstacles arose from limited stakeholder interaction, language barriers, and cultural, educational, and generational differences. Insufficient funding for human resources hindered communication opportunities, and the absence of previous research impact data caused frustration among decision-makers.
The study concludes that diverse communication methods are crucial for engaging BIPOC communities, emphasizing community involvement in selecting settings, language, and cultural activities. The findings have implications for policymakers, healthcare providers, community organizations, and researchers, guiding the development of targeted and effective health communication strategies to improve healthcare outcomes in BIPOC communities.
An Empirical Evaluation of Policy Options for Increasing Dairy Production in Indonesia: A System Dynamics Approach
Authors: Priyono Priyono , Nurmalina R , Burhanuddin Burhanuddin , Ilham N 
Published: March 2024

Domestic dairy production in Indonesia grows slower than consumption, resulting in an excess demand that imports must fulfill.Â Accelerating dairy production can no longer be solved partially; a holistic system approach is required.Â This study aims to empirically evaluate the policy options for accelerating dairy production in Indonesia. Â The data used in this study were secondary data from Badan Pusat Statistik, the Ministry of Agriculture, the Ministry of Trade, the Ministry of Industry, the Coordinating Ministry for Economic Affairs, the Ministry of Cooperatives and SMEs, Bank Indonesia, and FAO.Â A system dynamics approach was used to construct the model and describe the short-, medium-, and long-term impacts based on policy scenario options.Â The empirical results showed that the calf rearing program policy, increased female dairy cattle imports, higher conception rates, and import tariffs all positively impacted the share of domestic dairy production to dairy demand.Â On the other hand, a policy of increasing dairy consumption, if not accompanied by an increase in domestic dairy production, harms the domestic dairy production share.Â Except for the policy of increasing dairy consumption, all policy scenarios increased the long-term share of domestic dairy production more than the short- and medium-term. Â It is concluded that an optimistic policy through calf rearing, increased import of female cows, conception rate, and maintaining import tariffs was the best policy with the highest impact on increasing the share of domestic dairy production compared to pessimistic and moderate policies.
Multiplicity of semiclassical solutions for a class of nonlinear Hamiltonian elliptic system
Authors: Zhang Jian , Zhou Huitao , Mi Heilong 
Published: March 2024

Abstract
This article is concerned with the following Hamiltonian elliptic system:

−

ε

2

Δ
u
+
ε

b

→

⋅

∇

u
+
u
+
V

(

x

)

v
=

H

v

(

u
,
v

)

in

R

N

,

−

ε

2

Δ
v
−
ε

b

→

⋅

∇

v
+
v
+
V

(

x

)

u
=

H

u

(

u
,
v

)

in

R

N

,

\left\{\begin{array}{l}-{\varepsilon }^{2}\Delta u+\varepsilon \overrightarrow{b}\cdot \nabla u+u+V\left(x)v={H}_{v}\left(u,v)\hspace{1em}\hspace{0.1em}\text{in}\hspace{0.1em}\hspace{0.33em}{{\mathbb{R}}}^{N},\\ -{\varepsilon }^{2}\Delta v-\varepsilon \overrightarrow{b}\cdot \nabla v+v+V\left(x)u={H}_{u}\left(u,v)\hspace{1em}\hspace{0.1em}\text{in}\hspace{0.1em}\hspace{0.33em}{{\mathbb{R}}}^{N},\end{array}\right.

where

ε
>
0

\varepsilon \gt 0

is a small parameter,

V

V

is a potential function, and

H

H

is a super-quadratic sub-critical Hamiltonian. Applying suitable variational arguments and refined analysis techniques, we construct a new multiplicity result of semiclassical solutions which depends on the number of global minimum points of

V

V

. This result indicates how the shape of the graph of

V

V

affects the number of semiclassical solutions.