bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRIMING POTENCY OF THE PRODUCTS OF INDIVIDUAL REGIONS OF THE H‐2 COMPLEX AND THEIR ROLE IN THE RESTIMULATION IN SECONDARY MLC
Authors: Cukrová Věra , Rychlíková Milena 
Published: April 2007

SUMMARYGenetic analysis of the determinants causing an early proliferation in MLC after priming with allogeneic cells in vivo was performed. The active determinants were localized in the I‐A subregion of the H‐2 complex by using appropriate recombinant mouse strains.Upon priming with antigens coded by the whole H‐2 complex, the lymphocytes gave a strong MLC response only to the priming stimulator cells and cells sharing the I‐A subregion determinants of the latter. The response to third party stimulator cells and cells not sharing I‐A subregion determinants was low.Upon priming with various H‐2 region products, only lymphocytes primed against I‐A subregion products gave an early proliferative MLC response to the priming stimulator cells, whereas lymphocytes primed against other H‐2 region products gave none.
GENETIC ANALYSIS OF THE MIXED LYMPHOCYTE REACTION IN H‐1 SEROLOGICALLY IDENTICAL INBRED RATS
Authors: Bishop C. , Festenstein H. , Křen V. 
Published: April 2007

SUMMARYH‐1d and H‐11 serologically identical combinations of inbred rat strains were studied, in which there was a strong mixed lymphocyte reaction (MLR). The H‐1d identical combination was formed with the Maudsley reactive (MR) and BD IX strains, in which there was bidirectional stimulation. The LAD differences in the combination BD IX and MR were shown in experiments using back‐crosses derived from two different sources, BD IX x LEW and MR x LEW.The H‐11 identical combination was the F344 (Fisher) and LEW (Lewis) strains; here the MLR was unidirectional—LEW stimulating F344. Two other H‐11 serologically identical strains, AS and HCS, also stimulated F344 lymphocytes. Genetic segregation analyses using back‐crosses showed linkage with H‐1 in both instances. The LADs of AS, HCS and LEW appeared to be the same.These experiments show that the rat LADs so far detectable using these strains are all encoded by genes linked to the major histocompatibility system of the rat, despite serological identity. In addition to disassociation of the major histocompatibility system antigens detected by serological means and those defined by MLC testing, there was also disassociation of transplantation antigens as shown in the BD IX x MR model.
ABSENCE OF FOUR H‐2d ANTIGENIC SPECIFICITIES IN AN H‐2d SARCOMA
Authors: Garrido F. , Perez M. , Torres M. Dolores 
Published: April 2007

SummaryMCG4 is a BALB/c sarcoma induced as a solid tumour with 0.2 mgs of methylcholantrene. The primary tumour was serially transplanted subcutaneously in syngeneic mice. The ascites form obtained was used to study the expression of H‐2 antigeneic specificities in a postlabelling radioassay.MCG4 did not express H‐2D.4 (private specificity of H‐2d haplotypes) as well as H‐2.3, H‐2.8 and H‐2.13 (public specificities). In addition it expressed H‐2.5 (a public specificity not present in H‐2d cells). These results were confirmed by quantitative absorption analysis using MCG4 and positive‐negative normal lymphoid cells for a particular specificity.Results are discussed with regard to the control of expression of H‐2 antigens by regulatory genes.
FREQUENCY DISTRIBUTION OF HISTOCOMPATIBILITY‐2 ANTIGENIC SPECIFICITIES IN THE JAPANESE WILD MOUSE GENETICALLY REMOTE FROM THE EUROPEAN SUBSPECIES
Authors: Moriwaki K. , Shiroishi T. , Minezawa M. , Aotsuka T. , Kondo K. 
Published: April 2007

SummaryFifty‐eight Japanese wild mice, Mus musculus molossinus, collected from twenty‐eight localities were surveyed for twelve H‐2 antigens using the haemagglutination method. Significantly higher frequencies of H‐2.3 and H‐2.5 specifities and relatively lower frequencies of the other public specificities were observed. This was confirmed by examining four specificities, H‐2.3, 5, 13 and 23, in 370 mice. Quantitative absorption of the alloantisera by erythrocytes and spleen lymphocytes of molossinu mouse revealed definite absorption of H‐2.3, 5 and 8 antigens, though their antigenic strength was apparently weaker than the controls, B10 congenic mice. Comparative assay of the strength of H‐2.5 antigen in either homozygous and heterzygous conditions distinctly eliminated the possibility of gene‐dose effect for the reduced strength of the public antigens in molossinus mouse. To explain this, divergence time between molossinus and domesticus was computed based on the allelic frequency data already reported on ten loci in both subspecies. It is roughly 1.5 × 106 years, which could allow for the occurrence of considerable genetic changes in many public antigens, except those conserved through selection.
H‐2 LINKED IMMUNE RESPONSE TO MURINE EXPERIMENTAL SCHISTOSOMA MANSONI INFECTIONS
Authors: Claas F. H. J. , Deelder A. M. 
Published: April 2007

SummaryMice of two congenic inbred strains C3H/Sn (H‐2k) and C3H. B10 (H‐2b were infected with 100 Schistosoma mansoni cercariae. After the infection, the following parameters for the immunologica response were studied: worm burden, mortality, antibody titre, spleen index, eosinophilia, delayed type of hypersensitivity and in vitro response to three S. Mansoni antigen preparations.No difference in the worm burden and in the in vitro response to the antigen preparations of adult worm antigen, soluble egg antigens and the egg antilgen MSA1, was found. The C3H.B10 mice showed a singificantly higher mortality, antibody titre and delayed type of hyprsensitivity while the C3H/Sn mice showed a significantly higher spleen index and eosinophilia. This indicates that the H‐2 region influences the course of an acute S. mansoni infection, whereas the susceptibility to the infection seems not to be influenced, as is shown by the worm burden
A STATISTISTICAL ANALYSIS OF HUMAN LYMPHOCYTE TRANSFORMATION DATA
Authors: Harina B. M. , Gill T. J. , Rabin B. S. , Taylor F. H. 
Published: April 2007

SummaryThe lymphocytes from 107 maternal‐foetal pairs were examined for their in vitro responsiveness, as determined by the incorporation of tritiated thymidine following stimulation with phytohaemagglutinin (PHA), candida, varicella, mumps, streptokinase‐streptodornase (SKSD) and tetanus toxiod. The data were collected and analysed in two sequential groups (forty‐seven and sixty) in order to determine whether the results were reproducible. The variable chosen for analysis was the difference (d) between the square roots of the isotope incorporation in the stimulated and control cultures because it gave the most symmetrical distribution of the data. The experimental error in the determination of maternal lymphocyte stimulation was 1.4‐8.6% and of the and of the foetal lymphocytes, 1.0‐16.6% depending upon the antigen or mitogen and its concentration. The data in the two sets of patients were statistically the same in forty‐eight of the fifty‐six analyses (fourteen antigen or mitogen concentrations in autologous and AB plasma for maternal and foetal lymphocytes). The statistical limits of the distribution of responses for stimulation or suppression were set by an analysis of variance taking two standard deviations from the mean as the limits. When these limits were translated into stimulation indices, they varied for each antigen or mitogen and for different concentrations of the same antigen. Thus, a detailed statistical analysis of a large volume of lymphocyte transformation data indicates that the technique is reproducible and offers a reliable method for determining when significant differences from control values are present.
MOLECULAR PROPERTIES OF T‐LYMPHOMA IMMUNOGLOBULIN: II. PEPTIDE COMPOSITION OF THE HEAVY CHAIN
Authors: Moseley Jane M. , Beatty Elizabeth A. , Marchalonis J. J. 
Published: April 2007

SUMMARYIn order to obtain structural information and to facilitate studies of the covalent structure of T‐cell immunoglobulin, we have performed investigations of the peptide fragments of the heavy chain of this molecule, on a comparative basis, with heavy chains of serum immunoglobulins. T‐cell immunoglobulin was isolated from 125I‐labelled culture medium of monoclonal continuously cultured T‐lymphoma cells by means of immunoadsorbents. Peptides were prepared from the purified 125I‐labelled heavy chain by means of cleavage with cyanogen bromide or digestion with trypsin. We then resolved these peptides and compared them with those peptides derived from 131I‐labelled murine μ, γ and α chains separately and in mixed label experiments by means of polyacrylamide gel electrophoresis in sodium dodecyl sulfate containing buffers, 2 dimensional peptide mapping and ion exchange chromatography. The profiles of the radiolabelled peptides obtained from the T‐lymphoma heavy chains were quite distinct from those of murine γ chains but indicated a structural similarity to both μ and α chains, which share some common peptides.These results are consistent with the antigenic and physicochemical data available and suggest that T‐cell immunoglobulin is a new isotype that shows similarities to IgA and IgM, but of which the precise nature of the constant region has yet to be delineated.
GENETIC CONTROL OF SURVIVAL IN EPIDEMICS
Authors: De Vries R. R. P. , Khan P. Meera , Bernini L. F. , Loghem E. van , Van Rood J. J. 
Published: April 2007

SummaryDescendants of Dutch colonists, who emigrated to Surinam in the last century and survived epidemics of typhoid and yellow fever with a total mortality of about 60%, were tested for twenty‐six polymorphisms. The gene frequencies were compared with those of a large Dutch control sample. An analysis of drift indicated that the variations in gene frequencies observed for C3, Gm, HLA‐B, and GLO were unlikely to be due to drift. Therefore these data might indicate selection through genetic control of survival in these epidemics.
THE IMMUNOGLOBULIN‐LIKE T‐CELL RECEPTOR: II. CODISTRIBUTION OF Fab DETERMINANTS AND ANTIGEN ON THE SURFACE OF ANTIGEN‐BINDING LYMPHOCYTES OF MOUSE THYMUS*
Authors: DeLuca D. , Warr G. W. , Marchalonis J. J. 
Published: April 2007

SummaryChicken antibodies showing rigorous specificity for mouse immunoglobulins (Ig) allow the direct visualization of an Ig‐like surface component on thymusderived lymphocytes (T‐cells). These antibodies, therefore, provide a direct means of testing the hypothesis that the Ig‐like molecules function in antigen recognition on these cells. In an attempt to determine if avian antisera to mouse Fab determinants recognize antigen‐binding receptors, the surface distribution of three large fluorescent (red) protein antigens, keyhole limpet haemocyanin (KLH), horse spleen ferritin (HSF), and whale skeletal myoglobin (MYO) on BALB/c thymus antigen‐binding lymphocytes (ABL), was compared with that of fluorescent (green) labelled chicken antibodies to mouse Fab fragments [CAM(Fab')2]. The distribution of antigen and antisera against the Thy‐1 antigen and H‐2d determinants was also compared.The frequency of ABL ranged from 0.2 to 1% and binding to these cells could be inhibited by the appropriate unlabelled antigen and CAM(Fab')2. Thymus ABL were 100% positive for Thy‐1 antigen when doubly stained using fluorescent rabbit anti‐mouse immunoglobulin (RAM Ig) in an indirect fluorescence assay, but only 0‐10% of ABL were positive for binding of RAM Ig alone, indicating that nearly all ABL are T‐cells.Coincidence studies of patches of antigen and the various ligands were carried out by comparing coded fluorescence photomicrographs of randomly selected thymus ABL. Of thirty‐six ABL stained with anti‐thy‐1 antiserum, all but four cells had at least one patch of antigen which was not shared with antiserum. Of thirty‐six ABL stained with anti‐H‐2d serum, none had completely shared patches of antigen and antiserum. Of the forty ABL stained with CAM(Fab')2, every cell had every patch of antigen also patched with the anti‐Ig reagent. We intepret our results to indicate that the primary receptor for antigen on antigen‐binding T‐cells is either the Ig‐like molecule on the T‐cell surface or exists in close proximity to this molecule.
B‐COMPLEX GENETIC CONTROL OF IMMUNE RESPONSE TO HSA, (T,G)‐A–L, GT AND OTHER SUBSTANCES IN CHICKENS
Authors: Pevzner I. Y. , Trowbridge Carolyn L. , Nordskog A. W. 
Published: April 2007

SummaryImmune response to poly‐(l‐tyrosine‐l‐glutamic acid)‐poly‐d, l‐alanine‐poly‐l‐lysine ((T,G)‐A–L), human serum albumin (HSA), and (l‐glutamic acid50, l‐tyrosine50)n (GT) was found to be linked to the B complex in an outbred line of Leghorns segregating for the B1, B2, and B19 alleles. Birds of the blood group genotypes B1B1, B2B2, and B19B19 were low, intermediate, and high responders, respectively to either (T,G)‐A–L or HSA. Response to GT, however, differed, with the B2B2 genotype being the only responder. No real genotype differences in immune response to DNP‐conjugates and sheep red blood cells (SRBC) could be detected.
UNEXPECTED LYMPHO‐CYTOTOXIC REACTIONS OF ANTI‐H‐2 SERA ON NORMAL LYMPH‐NODE CELLS: ARE THEY DUE TO ALTERED H‐2 STRUCTURES OR ANTI‐VIRAL ANTIBODIES?
Authors: Ivanyi P. , Mourik P. van , Breuning M. , Melief C. J. M. 
Published: April 2007

SummaryWhen testing the serum of an individual anti‐H‐2 immunized mouse (B10 x A.SW)F1 anti‐B10.M by the routine micro‐lymphocytotoxicity test on lymph‐node cells, unexpected antibodies were found. The most striking finding was that after absorption of anti‐H‐2.8 antibodies with B10.A(2R) (Kk) cells, antibodies remained which reacted with AKR, B10.AKM and B10.A V + mice while B10.A V‐, B10.BR and C3H mice were negative. While all these strains share the Kk allele, only the positively reacting strains express high titres of infectious RNA turnover viruses. Unexpected reactions were observed also with H‐2d, H‐2j and H‐2r cells and absorption experiments indicated two or three antibody populations.These reactions could be interpreted by two different possibilities: (1) anti‐H‐2 antibodies react with virus‐altered H‐2 structures; and (2) antiviral antibodies react with H‐2 structures complexed with viruses. These possibilities should be taken into account when H‐2 sera are tested on tumour or virus‐infected cells.
A STRAIN SURVEY OF AGE‐DEPENDENT CHANGES IN ANTIGEN ELIMINATION, ANTIBODY FORMATION AND TOLERANCE
Authors: Nakano K. , Cinader B. 
Published: April 2007

SummaryA strain survey has been undertaken to examine polymorphism of age‐dependent changes in antigen elimination, immune responsiveness and tolerance inducibility. In young mice of tested strains, other than C57BL/6J, aggregate‐freed mouse Ig was eliminated faster than was the xenogeneic Ig (RGG); C57BL/6J was the only exception. The rate of elimination increased with age in all strains, tested. Antibody formation to RGG decreased in 3 (BALB/cJ, 129/J, DBA/2J) out of the eleven strains tested at 5‐7 and 31‐40 weeks of age; there was a considerable strain variation in the extent of this decrease. Age‐dependent resistance to tolerance induction was observed in a fraction of animals of H‐2 haplotypes, H‐2k and H‐2d.
GENE DELETION AND GENE DUPLICATION WITHIN THE CLUSTER OF HUMAN HEAVY‐CHAIN GENES: SELECTIVE ABSENCE OF IgG SUB‐CLASSES
Authors: Loghem Erna van , Sukernik R. I. , Osipova L. P. , Zegers B. J. M. , Matsumoto H. , Lange and Gerda de , Lefrance G. 
Published: April 2007

SummaryIndividuals with selective absence of IgG1 and IgG2 were discovered by testing for allotypes and isotypes of the respective sub‐classes. These individuals were homozygous for sub‐class deleted Gm‐Am haplotypes, as shown by allotype studies in two families (Gm‐;…;g;A2ml/Gm‐;n;b;A2ml and Gm‐;n;b;A2ml/Gm‐;…;b;A2ml) and by a population study of New Guineans (Gm fa;‐;b;A2m2). The individuals with IgGl sub‐class deficiency showed elevation of IgG2, IgG4 and in particular of IgG3.Gene deletion can result from unequal crossing over which renders a complementary chromosome with a duplication of a sub‐class gene. In one family, duplication of γ3 genes was observed to have happened in one of a twin pair. Quantitation of sub‐classes in families with γ1‐ and with γ3‐duplicated haplotypes did not show increased levels of the gene involved.
ALLOGENEIC RESTRICTION IN THE RAT: GENETIC BASIS OF RESTRICTION OF THE T CELL MEDIATORS OF DELAYED‐TYPE HYPERSENSITIVITY AND ANTIMICROBIAL RESISTANCE TO LISTERIA MONOCYTOGENES*
Authors: Jungi T. W. , McGregor D. D. 
Published: April 2007

SummaryThe genetic basis of the restriction imposed on T cell mediating acquired antimicrobial resistance and delayed‐type hypersensitivity (DTH) to Listeria in the rat was investigated. Sharing of MHC‐coded genes between donors of sensitized T cells and antigen‐stimulated recipients was both necessary and sufficient for efficient transfer of both resistance and DTH. Evidence to support this assumption was derived from experiments involving allogeneic transfers within major histocompatibility complex (MHC)‐compatible strains and across MHC‐barriers. Further support came from linkage studies with backcrossed rats and with the progeny of F1 rats mated with an unrelated strain. An unexpected difference in the compatibility requirements for effective transfer of DTH and resistance was noted in experiments involving the BI strain (formerly called B3). Thus, while B‐region compatibility was obligatory for expression of DTH in recipients of sensitized T cells, considerable levels of protection could be transferred to either A‐region or B‐region compatible hosts.
IMMUNOCHEMICAL PROPERTIES OF Mg ERYTHROCYTES
Authors: Dahr W. , Metaxas‐Bühler M. , Metaxas M. N. , Gallasch E. 
Published: April 2007

SummaryThe major erythrocyte membrane (MN) sialoglycoprotein in Mg red cells was found to exhibit a slightly decreased sodium‐dodecyl‐sulphate polyacrylamide gel electrophoretic molecular weight and periodic acid/Schiff staining intensity. Mg antigen activity was shown to be associated with this molecule. As judged from chemical modification experiments, no carbohydrate but the glycoprotein's N‐terminal amino acid is involved in the Mg receptor site. The endgroup of the glycoprotein was found to leucine and studies involving Staphylococcus aureus V8 protease suggest that a glutamic acid is located at the fitth position of its peptide chain. This indicates that the Mgs gene complex evolved from a mutation of an Ns allele. An amino acid substitution or deletion at the second, third and/or fourth position(s), preventing the glycosylation of all or some of these amino acids, provides an explanation for the properties of Mg erythrocytes.
GENETIC CONTROL OF ALLOGENEIC INTERACTIONS IN THE GUINEA‐PIG: II. EVIDENCE FOR ALLOANTIGEN‐SPECIFIC SUPPRESSOR CELLS IN THE GUINEA‐PIG MIXED LEUKOCYTE CULTURE
Authors: Geczy A. F. , Coulits Nelly 
Published: April 2007

SummaryThe cellular basis of B.2‐associated unresponsiveness in the guinea‐pig MLC has been investigated. Macrophage‐enriched and macrophage‐depleted lymphoid cells were treated with alloantisera and complement and the ability of these antiserum‐treated cell populations to present alloantigens to T lymphocytes investigated. It was found that B.2‐specific suppressor cells can be eliminated from macrophage‐depleted but not from macrophage‐enriched stimulator cells with anti‐B.2 serum and complement. By contrast, antiserum directed against allodeterminants on responding cells had no detectable influence on the MLC. Nylon wool column‐purified B.2‐specific T cells but not B.1‐specific T cells, suppress the MLC between B.1‐positive (stimulator cells) and B.2‐positive (responding cells) lymphoid cells. Furthermore, anti‐B.2 serum‐treated column‐purified B.2‐specific T cells lose their ability to suppress the MLC reaction. It is concluded that B.2‐specific MLC suppressor cells are T cells which are sensitive to lysis by alloantiserum in the presence of complement.
LOCATION OF LyM‐1 LOCUS ON MUS MUSCULUS CHROMOSOME 1
Authors: Sato H. , Natsuume‐Sakai S. , Katagiri M. , Itakura K. 
Published: April 2007

SummaryFollowing segregation of the alleles at the Sas‐1, LyM‐1 and M1s in the (AKR/J x C3H/HeJ)F1 x C3H/HeJ backcross progeny, we found the locus responsible for the lymphocyte alloantigen LyM‐1 to be closely linked to Sas‐1 and M1s. The map distances are estimated to be 12.5 ± 3.9 for Sas‐1 to LyM‐1, 6.9 ± 3.0 for LyM‐1 to M1s and 19.4 ± 4.7 for Sas‐1 to M1s. These data clearly indicate that the gene order is Sas‐1‐LyM‐1‐M1s on Mus musculus chromosome 1.
THE RARE Rh HAPLOTYPES ‐D‐ AND — IN A FAMILY WITH A ‐D‐/— PROPOSITUS
Authors: Kendrick L. , Dunstan‐Adams C. , Humphreys J. , Plantos M. , Moore B. P. L. , Lomas C. , Daniels G. L. 
Published: April 2007

SummaryA family is described in which the propositus and her compatible sister have the Rh genotype ‐D/—. The — haplotype is inherited from the paternal side and the ‐D‐ haplotype from the maternal side of the family.
GENETIC CONTROL OF THE IMMUNE RESPONSE TO MYOGLOBIN: VIII. CONTROL OF ANTIBODY AFFINITY
Authors: Young C. R. , O'Connor G. P. , Atassi M. Z. 
Published: April 2007

SUMMARYWe have previously shown that the antibody response to mammalian myoglobins is under genetic control. In the present study we examined antibodies to sperm‐whale, Atlantic bottlenosed dolphin, Black Sea dolphin, horse and badger myoglobins, raised in high responder strains of mice, to ascertain whether there is genetic control of antibody affinity to mammalian myoglobins. Using antisera of varying dilutions, the binding to 125I‐labelled homologous myoglobins was studied by inhibition with homologous myoglobin over a wide range of inhibitor concentration in a modified Farr assay. The results indicated that there are no large differences between high responder strains of mice in the affinity of antibodies to mammalian myoglobins.
IMPORTANCE OF NON‐H‐2 GENES IN THE CONTROL OF THE IN VIVO GROWTH OF THE VIRUS INDUCED LYMPHOMA YC8
Authors: Colombo M. P. , Pzarmiani G. 
Published: April 2007

SUMMARYThe genetic control of the in vivo growth of the Moloney virus‐induced BALB/c lymphoma YC8 was studied in F1 hybrids between BALB/c and several strains differing at the MHC and/or at the level of non‐H‐2 genes. Parental strains of the B10 and C3Hf but not of A, BALB/c or DBA/2 backgrounds introduced a significant resistance to the growth of 102 YC8 cells (a dose able to kill 100% of BALB/c mice) in semisyngeneic hosts. This resistance appeared to be due to non‐H‐2 genes although a modulation of the tumour growth by genes encoded by the MHC was also evident. The study of backcrosses between susceptible BALB/c and resistant (BALB/c x B10.BR)F1 crosses revealed that 83% of animals developed lethal tumors after injection of 102 YC8 cells. This high frequency of tumour takes was not linked to genes of the MHC. Adult thymectomy plus sublethal irradiation was able to abrogate the resistance of (BALB/c x B10.BR) or (BALB/c x B10.RIII)F1 mice to YC8 growth. Since the injection of silica also impaired the resistance to YC8, we tentatively concluded that the genetic control of resistance to YC8 is mediated both by T cells and macrophage‐like cells.
Gm, Am, Pi and Km MARKERS IN MESANGIAL IGA GLOMERULONEPHRITIS
Authors: Petit*, J. C. Le , Loghem, Erna Van, , Lange, Gerdade , Berthoux, F. C. , And Colette Chapuis Cellier , Serre J. L. 
Published: April 2007

SUMMARYNo. Gm, Am, Pi, and Km gene frequency difference has been observed between eighty‐seven Caucasin IgA nephropathies and ninety‐six controls. But a Km1 significant increase is found in patients with chronic renal failure compared with the total patients group. This data fits for a genetic predisposition factor in IgA nephropathy.