bluuebunny/crossref_metadata_2025_split huggingface dataset:
Space Durable 3D Printed High‐Performance Polymers Based on Cyanate Ester/Extended‐Bismaleimide
Authors: Grossman Eitan , Atar Nurit , Bolker Asaf , Riggs Brian E. , Minton Timothy K. , Gouzman Irina , Vidavsky Yuval 
Published: April 2024

AbstractTo accomplish the potential of the New‐Space emerging era and facilitate scientific and commercial space exploration, the development of versatile, customized, and affordable space technologies is essential. 3D printing is established as a disruptive technology, enabling the production of complex and lightweight structures with enhanced performance. However, the harsh conditions of the space environment, including atomic oxygen (AO), extreme temperatures, and ionizing radiation, pose significant challenges to the durability and longevity of additive manufacturing‐produced polymers. Until now, there are no additive‐manufacturing polymeric materials that are specifically developed and qualified to withstand space hazards. To address these challenges, novel materials for additive manufacturing, composed of cyanate ester and extended‐bismaleimide are engineered to withstand the extreme conditions in space. The developed materials demonstrate superior thermo‐mechanical properties (flexural stress of 72 MPa and Tg = 260 °C), enhanced durability to AO erosion, ionizing radiation (10 years in orbit), and thermal stability (Td5% = 360 °C). Moreover, it is found that printing orientation governs the AO erosion, thus guiding optimal printing designs for enhanced durability to AO. The materials show improved performance, endurance, and reliability, thus contributing to the development of space‐qualified components and enabling the advancement of additive manufacturing for future space missions.
Anchored Cobalt Nanoparticles on Layered Perovskites for Rapid Peroxymonosulfate Activation in Antibiotic Degradation
Authors: Wang Yaobin , Li Dong , Ge Xinlei , Yu Jianghua , Zhao Yunxia , Bu Yunfei 
Published: April 2024

AbstractIn the Fenton‐like reaction, revealing the dynamic evolution of the active sites is crucial to achieve the activity improvement and stability of the catalyst. This study reports a perovskite oxide in which atomic (Co0) in situ embedded exsolution occurs during the high‐temperature phase transition. This unique anchoring strategy significantly improves the Co3+/Co2+ cycling efficiency at the interface and inhibits metal leaching during peroxymonosulfate (PMS) activation. The Co@L‐PBMC catalyst exhibits superior PMS activation ability and could achieve 99% degradation of tetracycline within 5 min. The combination of experimental characterization and density functional theory (DFT) calculations elucidates that the electron‐deficient oxygen vacancy accepts an electron from the Co 3d‐orbital, resulting in a significant electron delocalization of the Co site, thereby facilitating the adsorption of the *HSO5/*OH intermediate onto the “metal‐VO bridge” structure. This work provides insights into the PMS activation mechanism at the atomic level, which will guide the rational design of next‐generation catalysts for environmental remediation.
Rhein‐based Pickering emulsion for hepatocellular carcinoma: Shaping the metabolic signaling and immunoactivation in transarterial chemoembolization
Authors: Liang Xiaoliu , Liu Hui , Chen Hu , Peng Xuqi , Li Zhenjie , Teng Minglei , Peng Yisheng , Li Jiwei , Ding Linyu , Mao Jingsong , Chu Chengchao , Cheng Hongwei , Liu Gang 
Published: April 2024

AbstractThe efficacy of transarterial chemoembolization (TACE) has been limited by insufficient embolization and a high incidence of tumor recurrence. Herein, we identified that aberrant metabolic reprogramming and immunosuppression contribute to TACE refractoriness and Rhein, as a potential glycolytic metabolism inhibitor and immunoactivation inducer, was optimized to sensitize tumors to TACE therapy. To achieve efficient embolization, we developed an oil‐in‐water lipiodol embolic emulsion by stabilizing the self‐assembled Rhein nanogel. The assembled Rhein exhibited a nanofiber network, and its integration enhanced the mechanical stability and viscoelasticity of the lipiodol embolic agent. With the synergistic advantages of solid and liquid embolic agents, this carrier‐free Pickering emulsion exhibits efficient embolization and sustained drug release in models of unilateral renal artery embolization, rabbit ear tumor embolization, rabbit orthotopic liver cancer, and rat orthotopic liver cancer. Compared to conventional three‐way catheter mixing methods, multimodal imaging corroborates a marked enhancement in local drug retention and tumor suppression. Importantly, the incorporation of Rhein‐mediated synergistic immunoembolization in this strategy achieved efficient embolization while robustly activating anti‐tumor immune responses, including inducing immunogenic cell death, dendritic cell activation, and major histocompatibility complex class I presentation to CD8+ T cells for tumor killing. Together, these findings reveal a novel strategy for the application of self‐assembled Rhein nanofiber‐stabilized lipiodol emulsion to control metabolic signaling and immunoactivation in TACE.
A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli
Authors: Zabolotskii Artur I. , Riabkova Natalia S. 
Published: April 2024

AbstractDue to the degeneracy of the genetic code, most amino acids are encoded by several codons. The choice among synonymous codons at the N‐terminus of genes has a profound effect on protein expression in Escherichia coli. This is often explained by the different contributions of synonymous codons to mRNA secondary structure formation. Strong secondary structures at the 5'‐end of mRNA interfere with ribosome binding and affect the process of translation initiation. In silico optimization of the gene 5’‐end can significantly increase the level of protein expression; however, this method is not always effective due to the uncertainty of the exact mechanism by which synonymous substitutions affect expression; thus, it may produce nonoptimal variants as well as miss some of the best producers. In this paper, an alternative approach is proposed based on screening a partially randomized library of expression constructs comprising hundreds of selected synonymous variants. The effect of such substitutions was evaluated using the gene of interest fused to the reporter gene of the fluorescent protein with subsequent screening for the most promising candidates according to the reporter's signal intensity. The power of the approach is demonstrated by a significant increase in the prokaryotic expression of three proteins: canine cystatin C, human BCL2‐associated athanogene 3 and human cardiac troponin I. This simple approach was suggested which may provide an efficient, easy, and inexpensive optimization method for poorly expressed proteins in bacteria.
Haplo‐insufficiency of Profilin1 in vascular endothelial cells is beneficial but not sufficient to confer protection against experimentally induced atherosclerosis
Authors: Allen‐Gondringer Abigail , Gau David , Dutta Partha , Roy Partha 
Published: April 2024

AbstractActin cytoskeleton plays an important role in various aspects of atherosclerosis, a key driver of ischemic heart disease. Actin‐binding protein Profilin1 (Pfn1) is overexpressed in atherosclerotic plaques in human disease, and Pfn1, when partially depleted globally in all cell types, confers atheroprotection in vivo. This study investigates the impact of endothelial cell (EC)‐specific partial loss of Pfn1 expression in atherosclerosis development. We utilized mice engineered for conditional heterozygous knockout of the Pfn1 gene in ECs, with atherosclerosis induced by depletion of hepatic LDL receptor by gene delivery of PCSK9 combined with high‐cholesterol diet. Our studies show that partial depletion of EC Pfn1 has certain beneficial effects marked by dampening of select pro‐atherogenic cytokines (CXCL10 and IL7) with concomitant reduction in cytotoxic T cell abundance but is not sufficient to reduce hyperlipidemia and confer atheroprotection in vivo. In light of these findings, we conclude that atheroprotective phenotype conferred by global Pfn1 haplo‐insufficiency requires contributions of additional cell types that are relevant for atherosclerosis progression.
Esophageal submucosal hematoma during transnasal endoscopy: A rare case report
Authors: Kanamori Atsushi , Nadatani Yuji , Kushiyama Nahoko , Nakata Akinobu , Higashimori Akira , Ominami Masaki , Kimura Tatsuo , Fukumoto Shinya , Fujiwara Yasuhiro , Watanabe Toshio 
Published: April 2024

AbstractEsophageal submucosal hematoma is a rare, often incidental complication of therapeutic endoscopic procedures marked by disrupted blood vessels beneath the esophageal mucosa, forming a hematoma. We report the unique case of a severely thin and alcoholic 38‐year‐old woman with a history of reflux esophagitis who developed an esophageal submucosal hematoma during an unsedated transnasal endoscopy for health check‐up. During the procedure, the patient experienced strong vomiting reflexes and vomited blood, leading to the initial suspicion of either Mallory‐Weiss syndrome or epistaxis. However, subsequent sedated endoscopy revealed an esophageal submucosal tumor‐like lesion and a mucosal laceration with blood clots, prompting a dual diagnosis of esophageal submucosal hematoma and Mallory‐Weiss syndrome. The bleeding was not severe enough to require hemostatic intervention. The patient opted for conservative treatment with vonoprazan, which resulted in the improvement and healing of the hematoma within 28 days. This is the first report of an esophageal submucosal hematoma during transnasal endoscopy and emphasizes the importance of including an esophageal submucosal hematoma and Mallory‐Weiss syndrome in the differential diagnosis of hematemesis encountered in similar scenarios. Factors such as severe thinness, daily alcohol consumption, and reflux esophagitis may have possibly contributed to the development of the esophageal submucosal hematoma in this patient.
Synthesis of Optically Pure Cyclometalated Iridium(III) Complex‐Peptide Hybrids and Their Anticancer Activity
Authors: Kanbe Azusa , Yokoi Kenta , Umezawa Masakazu , Tsuchiya Koji , Aoki Shin 
Published: April 2024

AbstractWe report on the synthesis of optically pure cyclometalated iridium(III) complex‐peptide hybrids as amphiphilic peptide conjugates (IPH‐ACs) and the effect of the stereochemistry with respect to their Ir(tpy)3 (tpy: 2‐(4′‐tolyl)pyridine) core on their cytotoxicity against cancer cells. Stereochemically pure IPH‐ACs were synthesized from Δ‐ and Λ‐Ir(tpyCO2H)3 by optical resolution via the diastereomeric intermediates that are conjugated with the chiral alcohol, (1R,2R)‐2‐aminocyclohexanol ((R,R)‐11), followed by the hydrolysis of the ester moieties, as we reported very recently, and their spectroscopic spectra are reported. It was found that both optically pure Δ‐ and Λ‐forms of IPH‐ACs induce paraptotic cell death in Jurkat cells and the EC50 values were evaluated by MTT assays. We also performed TEM (transmission electron microscope) analyses of Jurkat cells treated with Λ‐13 to observe morphological changes in paraptosis processes. The intracellular uptake of Λ‐forms of IPH‐ACs in the cells measured by ICP‐MS (inductively coupled plasma‐mass spectrometry) was higher than those of Δ‐forms and the EC50 values of the Λ‐forms were smaller than those of the Δ‐IPH‐ACs. The analysis of these results suggests that the intrinsic cytotoxicity is almost equal for the Δ‐ and Λ‐forms and that the difference in cytotoxicity against Jurkat cells is due to the selectivity in the intracellular uptake of each stereoisomer.
Identification, synthesis, and field evaluation of components of the female‐produced sex pheromone of Helopeltis cinchonae (Hemiptera: Miridae), an emerging pest of tea
Authors: Magsi Fida Hussain , Cai Xiaoming , Luo Zongxiu , Li Zhaoqun , Bian Lei , Xiu Chunli , Fu Nanxia , Li Jianlong , Hall David R , Chen Zongmao 
Published: April 2024

AbstractBackgroundHelopeltis cinchonae (Hemiptera: Miridae) is a major pest of tea plantations in Asia. Conventional control of pests with pesticides is unsustainable. Therefore, safe and eco‐friendly alternatives, such as pheromones, are required to manage the pest.ResultsIn gas chromatography–electroantennographic detection (GC–EAD) analysis of whole‐body extracts of virgin female H. cinchonae, two compounds elicited electroantennogram (EAG) responses from male antennae. These were identified as hexyl (R)‐3‐acetoxybutyrate and (R)‐1‐acetoxy‐5‐butyroxyhexane using gas chromatography–mass spectrometry (GC–MS) analysis compared to synthetic compounds. This is the first study to report 1‐acetoxy‐5‐butyroxyhexane as an insect pheromone component. The synthetic compounds elicited dose‐dependent EAG responses from the antennae of male H. cinchonae. In two field trapping experiments, the individual compounds were highly attractive to male H. cinchonae when dispensed from polyethylene vials. However, higher catches were obtained with blends of the two compounds in a 1:10 ratio. The blend of racemic compounds was as attractive as the blend of (R)‐enantiomers.ConclusionsWe reported that 1‐acetoxy‐5‐butyroxyhexane and hexyl 3‐acetoxybutyrate are components of the female‐produced sex pheromone of H. cinchonae, but further work is required on the blend and loading of pheromone and on trap design to provide an optimized system for monitoring and control of this pest. The results may also facilitate the identification of the pheromones of other Helopeltis species, which are major pests in many crops. © 2024 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Volatiles from essential oils of three Lamiaceae plants repel the winged cotton aphid, disturb its feeding behavior and reduce its fecundity
Authors: Zhang Ying , Zhang Tao , Wang Xinhang , Bian Zhipeng , Zhang Xiaofang , Yang Guoqing , Lu Yanhui 
Published: April 2024

AbstractBACKGROUNDInsects use odor detection to sense their surroundings. Use of volatile compounds, such as essential oils (EOs) of plants, to repel pests and disrupt their olfaction‐driven behaviors has great practical potential for use in integrated pest management. Despite the available information on the repellent effects of EOs on herbivorous insects, the olfaction‐based mechanisms remain unknown.RESULTSY‐tube olfactometer tests showed that the EOs of three Lamiaceae plants – Mentha arvensis L., Mentha piperita L. and Lavandula angustifolia Mill. – were significantly repellent to winged cotton aphid, Aphis gossypii Glover. Electrical penetration graph (EPG) tests indicated the EOs reduced phloem feeding and increased the level of non‐productive probing by the aphids. The EOs also reduced the fecundity of winged Aphis gossypii. Electrophysiological bioassays and gas chromatography–mass spectrometry (GC–MS) identified five physiologically active volatiles, that is menthone, isomenthone, neomenthol and menthol from Mentha piperita; menthone and menthol from Mentha arvensis; and linalool from L. angustifolia. Behavioral tests confirmed that all five compounds repelled winged Aphis gossypii. Under field conditions, the growth rate of aphid populations after 7 days was significantly lower in fields treated with these compounds than in the control fields.CONCLUSIONOur findings demonstrated that three EOs not only repelled winged Aphis gossypii but also interfered with the aphid's feeding behavior and reduced its fecundity. These EOs and their active constituents have great potential as eco‐friendly control products for use against Aphis gossypii. The effects of these EOs also exceed other repellents that only keep pests away from host plants. © 2024 Society of Chemical Industry.
Data‐driven iterative learning cooperative trajectory tracking control for multiple autonomous underwater vehicles with input saturation constraints
Authors: Wu Chengxi , Karimi Hamid Reza , Shan Liang , Dai Yuewei 
Published: April 2024

AbstractThis paper investigates the cooperative trajectory tracking (CTT) control problem of multiple autonomous underwater vehicles (AUVs). The multi‐AUV system is characterized by uncertain dynamics, being subjected to the impact about input saturation constraints and unmeasurable disturbances. First, a neural network‐based data‐driven control algorithm is proposed for the multi‐AUV system with unmeasurable disturbances and model parameters uncertain. The radial basis function neural network is employed to estimate the primary pseudo parameters of an equivalent data model, established through dynamic linearization methods. Subsequently, an iterative learning control approach based on adaptive gain is designed to act as a feedforward scheme along the iteration axis to enhance the tracking accuracy within a time constraint. Third, to prove that the resulting CTT control system fulfills the bounded stability under the proposed control approach, a formal stability analysis is provided. Finally, a simulation case study is conducted to illustrate the effectiveness of the proposed CTT control approach.
Understanding Macrophage‐Tumor Interactions: Insights from Single‐Cell Behavior Monitoring in a Sessile Microdroplet System
Authors: Lin Jiaxu , Zhang Qiang , Xie Tianze , Wu Zengnan , Hou Ying , Song Yang , Lin Yongning , Lin Jin‐Ming 
Published: April 2024

AbstractInteraction between tumor‐associated macrophages and tumor cells is crucial for tumor development, metastasis, and the related immune process. However, the macrophages are highly heterogeneous spanning from anti‐tumorigenic to pro‐tumorigenic, which needs to be understood at the single‐cell level. Herein, a sessile microdroplet system designed for monitoring cellular behavior and analyzing intercellular interaction, demonstrated with macrophage‐tumor cell pairs is presented. An automatic procedure based on the inkjet printing method is utilized for the precise pairing and co‐encapsulation of heterotypic cells within picoliter droplets. The sessile nature of microdroplets ensures controlled fusion and provides stable environments conducive to adherent cell culture. The nitric oxide generation and morphological changes over incubation are explored to reveal the complicated interactions from a single‐cell perspective. The immune response of macrophages under distinct cellular microenvironments is recorded. The results demonstrate that the tumor microenvironment displays a modulating role in polarizing macrophages from anti‐tumorigenic into pro‐tumorigenic phenotype. The approach provides a versatile and compatible platform to investigate intercellular interaction at the single‐cell level, showing promising potential for advancing single‐cell behavior studies.
Deciphering the prognostic landscape of osteosarcoma: Integrating the roles of hippo pathway genes, programmed cell death, and the tumor immune microenvironment
Authors: Ma Huayan , Zhang Yuxian , Li Jing , Xie Tiantian , Zhao Zhi 
Published: April 2024

AbstractOsteosarcoma is a highly aggressive cancer prevalent among adolescents and young adults, notorious for its tendency to metastasize to the lungs. This research delves into the molecular foundations of osteosarcoma by examining the role of the Hippo signaling pathway and its interaction with the tumor immune microenvironment (TME). Through analysis of transcriptomic data from the TARGET‐OS dataset and control samples from GTEx, we identified a set of 131 genes that link high expression profiles in osteosarcoma with the Hippo pathway. A focused examination through univariate Cox regression analysis revealed eight key genes (DLG5, WNT11, TGFB2, DLG4, WNT16, ID2, WNT10B, and WNT10A) with a significant correlation to patient outcomes. Hierarchical clustering of these genes delineated two distinct patient groups with significantly different survival rates, a finding supported by Kaplan–Meier survival analysis. Further investigation into immune cell infiltration and expression profiles of immunoregulatory factors uncovered a notable pattern of immune evasion in the group with poorer prognosis, marked by reduced effector immune cell activity and lower levels of immunostimulatory factors. Single‐cell sequencing highlighted the cellular diversity within osteosarcoma samples and identified markers differentiating malignant from nonmalignant cells, correlating these markers with prognostic risk scores. Our results emphasize the critical prognostic value of Hippo pathway genes and the TME in osteosarcoma, shedding light on new avenues for therapeutic intervention and patient‐specific treatment strategies.
Risk perception and food safety practices among Slovenian pet owners: does raw meat feeding of pets make a difference?
Authors: Ovca Andrej , Bulochova Veronika , Pirnat Teja , Evans Ellen W. 
Published: April 2024

AbstractCommercially produced and home-made raw meat-based pet diets are becoming increasingly popular and have been recognised as an emerging public health risk by the European Food Safety Authority. The aim of this study was to investigate risk perceptions and self-reported pet food preparation practices in the home setting among pet owners who feed raw meat-based diet to pets. An online questionnaire was distributed to pet owners through social media groups. Ultimately, 750 respondents were included in the detailed analysis, divided into two subgroups, a “raw group” consisted of pet owners who provided raw meat-based diets for pets (n = 382), while the “conventional group” consisted of pet owners who did not (n = 368). There was no significant difference between the two groups in overall risk perception. Only one third of respondents in both groups were aware of the potentially fatal consequences of food poisoning. Self-reported frequency of hand washing, and surface cleaning, were significantly higher (p = 0.000) in the raw group than the conventional group. Food safety malpractices were noted in the raw feeding group, with 47% reporting to rinsing raw peat meat before preparing it, and 42% thawing frozen raw peat meat at room temperature on the kitchen counter. The results provide information on the prevalence of feeding raw meat to pets among Slovenian pet owners and provides the basis for developing tailored food safety education campaigns to improve risk awareness and provide food safety recommendations for pet owners providing raw meat-based diets for pets in Slovenia.
A Fully Coupled Numerical Model for Unbonded Post-tensioned Timber Structures
Authors: Dinçer A. Ersin , Demir Abdullah 
Published: April 2024

AbstractThe paper presents a fully Lagrangian mesh-free solver to simulate the dynamic behavior of post-tensioned timber structures. Weakly Compressible Smoothed Particle Hydrodynamics (SPH) is employed to model both the timber and the tendon. An efficient and simple coupling method between the timber and the tendon is proposed by considering the numerical stability. Besides, the same coupling algorithm is used to model the interaction between column and beam elements. Although the column is treated as rigid in the simulations, the coupling algorithm accounts for the initial compression of the column resulting from post-tensioning. For the verification of the code for solids and material nonlinearity of timber, benchmark problems available in the literature are used. Finally, the solver's capability is demonstrated through dynamic analysis of post-tensioned timber structures. The solutions obtained for all the cases are in good agreement with the experimental and theoretical data, which indicates the applicability and accuracy of the solver.