bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intestinal Epithelial Cell-specific Knockout of METTL3 Aggravates Intestinal Inflammation in CLP Mice by Weakening the Intestinal Barrier
Authors: Shi Hongzhou , Sun Jiahui , Sun Yaya , Wu Junjie , Jiang Guangqing , Xu Zhaiyue , Shi Xin , Fang Miao 
Published: March 2024

Background:Many studies have demonstrated that the expression of methyltransferase- like 3 (METTL3) is altered in various inflammatory diseases. Its specific mechanistic role in the intestinal inflammatory response during sepsis remains limited and requires further investigation.Objectives:Explore the potential mechanism of METTL3 in the intestinal inflammatory response during sepsis.Materials and Methods:Immunohistochemical analysis was utilized to detect the expression of METTL3 in the necrotic intestine of patients with intestinal necrosis and the small intestine of cecal ligation and puncture (CLP) mice. Mice were subjected to the CLP and Sham surgeries, intestine tissue was harvested and performed HE staining, and ELISA to examine intestinal inflammatory responses, while TUNEL staining was applied to detect intestinal cell apoptosis. Additionally, ELISA was used to detect diamine oxidase (DAO) and intestinal fatty acid binding protein (I-FABP) levels in intestinal tissue. Immunohistochemistry and RT-qPCR were also employed to examine the mRNA and protein expression levels of Zona Occludens 1 (ZO-1) and Claudin-1. Finally, transcriptomic sequencing was performed on the small intestine tissues of METTL3 Knock-out (KO) and Wild-type (WT) mice in response to sepsis.Results:METTL3 exhibited lower expression level in the necrotic intestine of patients and the small intestine of CLP mice. Loss of METTL3 in CLP mice triggered significantly higher expression of TNF-α and IL-18, down-regulated expression of ZO-1 and claudin-1, and decreased expression of DAO and I-FABP in the intestinal tissue. KEGG enrichment analysis showed that the differential genes were significantly enriched in immune-related pathways.Conclusion:This study reveals a novel mechanism responsible for exacerbated intestinal inflammation orchestrated by METTL3. Particularly, METTL3 null mice displayed decreased ZO- 1 and Claudin-1 expression, which largely hampered intestinal epithelial barrier function, resulting in bacterial and toxin translocation and intestinal immune activation and inflammation against sepsis.
Retrospective study of acid-base disturbances in patients with chronic kidney disease
Authors: Denova L.D. , Ivanov D.D. 
Published: March 2024

Background. Impaired kidney function has a negative effect on the body’s acid-base status (ABS). However, a violation of ABS can worsen the course of chronic kidney disease (CKD). Therefore, during the examination and treatment of patients with CKD, it is necessary to take into account the ABS. The purpose of the study: to conduct a retrospective analysis of the dynamics of ABS indicators in patients with stage 1–5 CKD who sought outpatient medical care in a medical institution in Kyiv. Materials and methods. A retrospective, randomized, cohort study was performed of 53 medical records of patients (form 025/o) who sought outpatient medical care at the Professor Dmytro Ivanov Nephrological Clinic LLC with a diagnosis of stage 1–5 CKD from February 2022 to February 2023. Participants were examined for ABS indicators three times. They were divided into 2 groups: group 1 (n = 31) — stage 1–3 CKD and group 2 (n = 22) — stage 4–5 CKD. An analysis of ABS indicators was carried out in dyna­mics. Results. Results of multiple linear regression for patients with stage 1–5 CKD (n = 53) showed that there was a strong significant effect between potassium (K+) and glomerular filtration rate (GFR) (F(1, 13) = 10.59, p = 0.006, R2 = 0.45, R2 adj = 0.41). The results of multiple linear regression of indicators in patients with stage 1–3 CKD (group 1) demonstrated a weak insignificant influence between lactate and GFR (F(1, 15) = 1.11, p = 0.310, R2 = 0.07, R2 adj = 0.01). The results of multiple linear regression of indicators in patients with stage 4–5 CKD (group 2) showed that there is a very strong combined insignificant influence between the hydrogen index, partial pressure of carbon dioxide, partial pressure of oxygen, bicarbonate concentration, base excess of extracellular fluid, K+, total carbon dioxide, anion gap with K concentration included in the equation, base excess, lactate and GFR (F(10, –3) = 0.3, p = 58.099, R2 = –65.23, R2 adj = 155.53). Conclusions. When studying ABS in patients with stage 1–5 CKD, a correlation was found between K+ and GFR using the multiple linear regression tool. In the first group, the relationship between lactate and GFR, in the second one, all predictors had a relationship with GFR.
How and why did the transition process towards a fully sustainable production of cocoa beans in Bahia (Brazil) take place?
Authors: König Claudia Cheron , Macchione Saes Maria Sylvia , Schnaider Paula Sarita Bigio 
Published: March 2024

Abstract
This paper theoretically models and empirically assesses the sociotechnical transition process experienced by a group of producers in southern Bahia State (Brazil). Driven by the search for economic sustainability in cocoa production, this group has achieved full sustainability through production chain upgrading, enabling the agroforestry system’s (cabruca) continuity in their properties. In order to understand how and why this process happened, our conceptual model advances on the Multi-Level Perspective (MLP) framework, as it is an applied way of analyzing different niche transformations within the same regime, a typical situation in agrifood systems in emerging markets. Our analyses and empirical evidence suggest that interpersonal relationships play a crucial role in this process, particularly developing concepts and strategies, and in establishing operational structures. Rules and structure formalization may replace interpersonal relationships, as observed when the transition goes forward. Our findings evidence knowledge gathering, cooperation among actors, and information and support exchanging as critical factors to trigger the niche transition process.
Haptoglobin Phenotype and Intensive Glycemic Control for Coronary Artery Disease Risk Reduction in People With Type 2 Diabetes: The ADVANCE Study
Authors: Cahill Leah E. , Warren Rachel A. , Carew Allie S. , Levy Andrew P. , Sapp John , Samuel Michelle , Selvin Elizabeth , Lavallée Samantha K. , Poulter Neil , Marre Michel , Harrap Stephen , Mancia Giuseppe , Harris Katie , Chalmers John , Woodward Mark , Rimm Eric B. 
Published: March 2024

OBJECTIVE
Intensive glycemic control reduced coronary artery disease (CAD) events among the Action to Control Cardiovascular Risk in Diabetes (ACCORD) study participants with the haptoglobin (Hp)2-2 phenotype but not in participants without the Hp2-2 phenotype. It is unknown whether and how these results translate across different demographic/clinical characteristics and treatment strategies.

RESEARCH DESIGN AND METHODS
Haptoglobin phenotype was measured in available samples from the Action in Diabetes and Vascular Disease: Preterax and Diamicron MR Controlled Evaluation (ADVANCE) biomarker case-cohort study. Weighted multivariable-adjusted Cox regression models were used to evaluate the association between intensive glycemic control (HbA1c target of ≤6.5%) versus standard therapy (based on local guidelines) and major CAD events among participants with (n = 1,327) and without (n = 2,077) the Hp2-2 phenotype separately and within prespecified stratifications by sex, race, previous cardiovascular disease (CVD), diabetes duration, and HDL-cholesterol.

RESULTS
While the hazard ratios (HRs) were in the hypothesized differing directions, compared with standard therapy, intensive glycemic control was not significantly associated with risk of CAD events among participants without (1.04, 95% CI 0.82–1.32) or with (0.84, 0.63–1.14, Pinteraction = 0.27) the Hp2-2 phenotype overall. Intensive therapy was associated with lower CAD risk among participants with the Hp2-2 phenotype who had no previous CVD (0.47, 0.29–0.76, Pinteraction = 0.01).

CONCLUSIONS
Our findings suggest that intensive glycemic control contributes to the prevention of major CAD events among ADVANCE participants with the Hp2-2 phenotype and no previous CVD and are in alignment with our hypothesis that intensive glycemic control may be beneficial in a subset of people with the Hp2-2 phenotype.
Nitrogen Management and Virus Incidence on Cut Flower Production of Dahlia
Authors: Oliver Frank , Stock Melanie , Nischwitz Claudia 
Published: March 2024

AbstractCut flower production, particularly of dahlia, has a strong profit potential for small farms. This study evaluated the field response of dahlia ‘Café au Lait’ to nitrogen (N) rates and implemented routine testing for common viruses. Yield was measured over a three-year field trial with five N application rates: 0, 56, 112, 168, and 224 kg N·ha−1 (0, 50, 100, 150, 200 lb N·A−1) in North Logan, Utah (USDA Hardiness Zones 5). A grower-participant study was also conducted in Northern Utah (USDA Hardiness Zones 5 – 7) to understand cultural practices and challenges. Application of 168 and 224 kg N·ha−1 (150 and 200 lb N·A−1) produced the greatest yields of stems per plant, while 168 kg N·ha−1 (150 lb N·A−1) was the most economically efficient from partial economic budgets. Viruses were highly prevalent in stock, resulting in cull rates of nearly 50% in 2019 and 2020. After sourcing lab-cultured stock in 2021, 0% of plants were culled, indicating a strong need for management of viruses in dahlia. Soil survey results from the grower-participant study revealed widespread levels of elevated macronutrients and salinity that increased annually and correlated with decreasing yield across farms. This study helps determine optimal N rates, increase awareness of virus management, as well as supporting fertility outreach with small growers to promote soil sustainability.Species used in this study: Dahlia, Dahlia pinnata ‘Café au Lait’.
Road traffic accidents, sanctions and delays in judgments in Court proceedings in Prizren - Republic of Kosovo
Authors: Beti Ejup 
Published: March 2024

Abstract
Traffic accidents are unexpected and unpleasant cases on the road, which often have consequences of damage to cars, people’s injuries and in more severe cases lead to death. These events occur for various reasons and can be the result of factors such as high speed, disregard for traffic signals, alcohol, unused safety belt, technical vehicle problems, bad weather conditions and dangerous drivers’ behavior. The consequences of traffic accidents can be very serious, including serious physical injuries, material damage to the vehicle, and in some cases loss of life. Some of the preventive measures to reduce the risk of traffic accidents include the respect for traffic rules, use of seat belts, non-consumption of alcohol at the time of driving, car maintenance, education and information. Road safety is a common challenge that requires cooperation and dedication from drivers, road authorities and the community in general. This challenge is faced by law enforcement institutions to perform the tasks and obligations deriving from the legislation in terms of discouraging legal violations by traffic participants. Therefore, this paper is intended for the discovery and interpretation of any stagnation or negligence that can lead to the encouragement of traffic participants to neglect traffic rules and laws. The analysis of this manuscript is accompanied with statistics, tables and graphs from 116 cases from a population of about 150,000 inhabitants.
The State of The Topiaj During 14th Century
Authors: Qerimi Muhamet , Zeneli Fidan 
Published: March 2024

Purpose: The paper provides historical context on the Topias family's role in Naples' political and religious landscape, detailing their break from Byzantium, alliances with Anjouans, and power dynamics.

Methods: The paper discusses the Topias family's historical context, indicating that historical scholars used a combination of archival research, primary source analysis, comparative analysis, contextualization, and narrative storytelling to understand their interactions, relationships, and historical context. These methods were likely used to gather and analyze information about the family's history.

Results and Conclusion: The paper provides a narrative account of the Topias family's historical significance, their break from Byzantium, and their role as vassals to the king of Naples. It highlights their resilience and adaptability in medieval European politics, highlighting the complex dynamics of political and religious spheres.

Research implications: The paper provides a rich historical account of the Topias family, highlighting their political strategies, interfaith relations, vassalage, Anjouan influence in the Balkans, and the influence of clergy in politics. It also highlights the importance of examining the historical narrative and its potential to contribute to ongoing scholarly discussions on medieval European history, politics, and culture.

Originality/value: The paper provides valuable insights into medieval Balkan politics, interfaith relations, and vassalage dynamics in medieval Europe. It focuses on lesser-known actors like the Topias family, their religious conversion, Angevin influence in the Balkans, and the complexities of feudal relationships. The narrative also highlights the intersection of clerical and political power, enhancing our understanding of medieval governance and interfaith relations.
Indonesian Government Policy in Increasing State Revenue Through Raw Material Export Prohibitions
Authors: Setyagama Azis , Susilo Wawan , Purwanto  , Su’ud Muchammad 
Published: March 2024

Purpose: To increase state income and revenues by prohibiting the export of raw materials, especially mining and mineral materials, by converting raw materials into semi-finished or finished materials so that added value can be added.

Method: This study uses normative legal research which examines the policies of the Indonesian government as outlined in Law No. 4 of 2009 concerning Minerals and Coal, which prohibits raw mining and mineral materials from being exported abroad. The research approach is through a qualitative juridical approach, namely by examining it from a legal standpoint as well as providing explanations related to the ban on the export of minerals and minerals.

Results and Conclusions: The policy of prohibiting the export of raw mining and mineral materials generates greater state income and revenue, because it gets a large added value by imposing high taxes, and also opens up employment opportunities and reduces unemployment in Indonesia.

Research implications: The Indonesian government's policy of prohibiting the export of mineral and mineral raw materials raises pros and cons in the country, but the Indonesian government remains adamant that this policy is to increase state revenues and revenues, added value and employment. Overseas, the government's policy of banning the export of mineral and mineral raw materials is opposed by the United States and the European Union because it will harm them as importers of mineral and raw materials from Indonesia

Originality/value: Importing countries of mining and mineral raw materials such as China, the United States and Europe have established mining and mineral raw material (Smelteer) processing companies in Indonesia.
Influence of Organic Granular Fertilizer, Chemical and Granular Organic Fertilizer with Hormone Mixed Formula (Ho), and Chemical Fertilizer to the Yield and Starch Content of Cassava
Authors: Raksarikorn Chawalit , Pumisak Intanon , Ruankuan Intanon 
Published: March 2024

Objective: The purpose of this study was to the influence of fertilizers and the use rate of fertilizer for soil improvement on the yields, and starch content of cassava.

Method: The experiment design was in RCBD with 9 treatments, which were as follows; T1 (Control), T2 (ORG 50 kilograms/rai.), T3 (ORG 100 kilograms/rai.), T4 (HO-1 50 kilograms/rai.), T5 (HO-1 100 kilograms/rai.), T6 (HO-2 50 kilograms/rai.), T7 (HO-2 100 kilograms/rai.), T8 (CHEM 50 kilograms/rai.), and T9 (CHEM 100 kilograms/rai.) The experiment plots were located at Pasao, Muang District, Uttaradit Province, from June 2017 – June 2018. by using the DMRT method at 95% confidence.

Results and Conclusions: The result showed that yield components, the use of HO-2 fertilizer 100 kilograms/rai. The formula of yield quality for cassava. It has the mean number of tubers per plant 17.00 tuber/ plant, number of root length 47.75 cm., and the cassava tuber circumference 25.25 cm. significantly different at 95%. While the components of average weight/tuber 0.67 kilograms/tuber and yield weight/tree 10.55 kilograms/plant were the highest among other treatments. But there was no statistical difference at 95%. However, when considering the highest average yield per rai, It was found that using HO-2 fertilizer 100 kg/rai gave a higher yield than other treatments (5,110 kilograms/rai). The rate of 100 kilograms/rai resulted in a higher percentage of cassava starch content than other methods (30.50%), which was statistically significantly different at 95%, better than using chemical fertilizers, organic granular fertilizers, and without fertilizers (Control). In terms of cost and profit, It was found that using 100 kg/rai of HO-2 fertilizer resulted in the highest profit from product sales per rai of 10,458 baht/rai, which was higher than using chemical fertilizers and organic granular fertilizer. Moreover, HO fertilizer was a choice to manage soil and fertilizer that affects the cassava product quality in Uttaradit Province.

Research implications: The hormone mixed formula (HO) fertilizer 100 kilograms/rai had certain impact on the improvement on chemical properties and the soil physical appearance than not putting the fertilizer, that the HO-2 100 kilograms per rai was suitable for increasing the productivity and percentage of starch content of cassava.
Originality/value: This study evaluates fertilizer types and application rates on cassava yield and starch content in Uttaradit Province, Thailand. Using a randomized complete block design, it provides insights into soil fertility and productivity improvement. The study also assesses profitability, offering practical insights for farmers.
Comparative assessment of the change in the species composition of vegetation on the territory of the western field of RSAU-MTAA
Authors: Spynu М. Т. , Titova М. I. , Seregin I. A. , Yaroslavtsev А. М. , Aleksandrov N. А. 
Published: March 2024

The article presents the analysis of changes in the floral composition of the western field of the Ecological Station of the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy for 4 years. The research object is located in the center of the megalopolis, it is subject to high anthropogenic load, as it is surrounded on both sides by a road network, and at the same time it is part of the facies of the Forest Experimental Dacha. The site is characterized by close occurrence of groundwater, high density of upper soil horizons, heterogeneous soil structure and mesorelief, resulting of in flooding. As a result of a significant number of anthropogenic and abiotic factors, it is necessary to analyze the vegetation composition and the species richness of the area in order to assess the dynamics of variability of these conditions and to predict the potential stability of the biological diversity of the area. The changes in the species composition were analyzed by describing the vegetation and evaluating quantitative indicators: frequency of occurrence, abundance of species, degree of their dominance according to the O. Drude scale, as well as the Shannon index and indicators of species richness and saturation.The studies carried out showed a pronounced variability in vegetation cover from 2018 to 2022. During the study period, there was a change in plant families and an increase in species diversity: richness and saturation indices. In 2022, there was an increase in the number of species and an increase in the uniformity of their numbers compared to 2018.
ANÁLISE ESTATISTICA DA MORFOMÉTRIA DAS BACIAS DOS RIOS DA VERTENTE SÃO FRANCISCANA NO ESTADO DE ALAGOAS
Authors: Vieira Vânia Priscila , Almeida Flávia Silva , Ferreira Neto José Vicente 
Published: March 2024

A regionalização de área na geografia está ligada diretamente a dados econômicos e de saúde, onde são determinados conglomerados para uma melhor implementação de políticas públicas direcionadas. O agrupamento de bacias hidrográficas é uma prática aplicada no brasil para um melhor gerenciamento e gestão dos recursos hídricos. Baseado no aspecto de vizinhança, essa prática nem sempre tem uma semelhança morfométrica. As bacias hidrográficas, normalmente, são fontes de renda para vários municípios devido sua grande importância se faz necessário um estudo mais detalhado sobre a morfometria das bacias hidrográficas, pois através desses estudos é que se pode prever sua capacidade e assim regionaliza-las. Os parâmetros morfométricos extraídos de imagens de satélites para a caracterização de bacias hidrográficas convencionalmente é utilizado para a determinação de suas capacidades hídricas. O presente estudo teve como objetivo definir uma regionalização para a vertente são franciscana do estado de alagoas com base na similaridade morfométrica das bacias hidrográficas, fazendo-se uso das geotecnologias. Os resultados indicam uma agregação de bacias com similaridades, apresentando assim uma nova proposta para a regionalização da vertente sõ franciscana do estado de alagoas, permitindo uma divisão mais eficaz e servindo como ferramenta para um planejamento fisico-territorial adequado com aplicação inclusive de práticas conservacionistas. What do you want to do ?New mailCopy What do you want to do ?New mailCopy What do you want to do ?New mailCopy
A GEOGRAFIA ECONÔMICA NO ENSINO DE GEOGRAFIA
Authors: Mendes Hinckley Wendell do Nascimento 
Published: March 2024

Este trabalho busca apresentar a importância da geografia econômica na educação básica para fortalecer o entendimento dos discentes sobre a Geografia enquanto ciência. O objetivo deste artigo é analisar o papel da geografia econômica para que se compreenda a importância da Geografia na educação básica; para melhor compreender este artigo, existem dois objetivos específicos: entender como a geografia econômica une as duas vertentes de análise da Geografia – Geografia Humana e Física; e o segundo objetivo, é compreender a Geografia como ciência fundamental ao desenvolvimento e progresso da humanidade. Para realização deste trabalho utiliza-se os seguintes procedimentos metodológicos: pesquisas bibliográficas sobre o ensino da Geografia e a geografia econômica encontrados nos sites acadêmicos do Ministério da Educação, UFSC, Scielo; entre outros. Assim, o artigo apresenta a relevância da geografia econômica no ensino da Geografia, principalmente para estudantes de educação básica.