bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Wnt5b‐Ror1 signaling in the proliferation of pancreatic ductal adenocarcinoma cells
Authors: Yamauchi Natsuko , Otsuka Mako , Ishikawa Tomohiro , Kakeji Yoshihiro , Kikuchi Akira , Masuda Atsuhiro , Kodama Yuzo , Minami Yasuhiro , Kamizaki Koki 
Published: March 2024

AbstractPancreatic ductal adenocarcinoma (PDAC) is one of the most refractory cancers with the worst prognosis. Although several molecules are known to be associated with the progression of PDAC, the molecular mechanisms underlying the progression of PDAC remain largely elusive. The Ror‐family receptors, Ror1 and Ror2, which act as a receptor(s) for Wnt‐family ligands, particularly Wnt5a, are involved in the progression of various types of cancers. Here, we show that higher expression of Ror1 and Wnt5b, but not Ror2, are associated with poorer prognosis of PDAC patients, and that Ror1 and Wnt5b are expressed highly in a type of PDAC cell lines, PANC‐1 cells. Knockdown of either Ror1 or Wnt5b in PANC‐1 cells inhibited their proliferation significantly in vitro, and knockout of Ror1 in PANC‐1 cells resulted in a significant inhibition of tumor growth in vivo. Furthermore, we show that Wnt5b‐Ror1 signaling in PANC‐1 cells promotes their proliferation in a cell‐autonomous manner by modulating our experimental setting in vitro. Collectively, these findings indicate that Wnt5b‐Ror1 signaling might play an important role in the progression of some if not all of PDAC by promoting proliferation.
Women and girls with inherited bleeding disorders: Focus on haemophilia carriers and heavy menstrual bleeding
Authors: Hermans Cedric , Johnsen Jill M. , Curry Nicola 
Published: March 2024

AbstractRaising awareness and improving recognition, accurate classification, and enhanced access to new treatments represent current key challenges for carriers of haemophilia. Women and girls carrying genes for haemophilia often experience significant bleeding and/or low factor levels. The bleeding associated with female haemophilia is frequently overlooked, has a weak correlation with factor levels, and manifests differently than in males, with heavy menstrual bleeding being a predominant symptom. Recent changes in terminology now allow the diagnosis of haemophilia in females with low factor levels and differentiate between symptomatic and asymptomatic carriers of the gene.Observations from real‐world experiences and limited clinical trial data have highlighted the positive impact of various new haemophilia treatments for women and girls with clotting factor deficiencies. There is an urgent need for initiatives that increase their access to these treatments and encourage well‐designed clinical trials focusing on female‐specific outcomes.In women with inherited bleeding disorders, early recognition and optimal management of heavy menstrual bleeding are crucial. However, treatment options and guidance from high‐quality clinical trials are currently insufficient. Menstrual health assessment should be a regular part of monitoring women and girls with inherited bleeding disorders throughout their lives, emphasizing the importance of gathering data to improve future management.
Health‐risk behaviours co‐occur among adults with tooth loss
Authors: Alzahrani Saeed G. , Rijhwani Kavita , Sabbah Wael 
Published: March 2024

AbstractIntroductionHealth‐risk behaviours tend to co‐occur among the same sectors of the population. The aim of this study is to examine the association between an aggregate of multiple health‐risk behaviours and tooth loss among American Adults.MethodsWe used data from the Behavioural Risk Factor Surveillance System (BRFSS) 2022, a nationally representative survey of non‐institutionalized American. We included participants aged 18 years and older. An aggregate variable of health‐risk behaviours which included smoking, heavy alcohol consumption, lack of physical activities, overweight/obesity, infrequent dental visits and infrequent medical check‐up was created. Tooth loss was indicated by losing one tooth or more. Logistic Regression analysis was conducted to test the association between the aggregate of behaviours and tooth loss adjusting for income, education, ethnicity and health insurance.ResultsThe analysis included 326,561 participants. The mean number of health‐risk behaviours was 2.13 and 1.72 among participants with tooth loss and without tooth loss, respectively. The aggregate of health‐risk behaviours was significantly associated with tooth loss with odds ratios 1.23 (95% CI, 1.21, 1.26) in a model adjusting for age, gender, education, income ethnicity and health insurance.ConclusionThis study demonstrated that an aggregate of health‐risk behaviours, with some not directly linked to oral health, is associated with tooth loss among American adults. The study highlights the importance of considering different risk factors when planning health promotion policies to tackle oral health.
Language and thought: Linguistic relativity in social marketing communication
Authors: Goel Manvi , Sharma Vinay , Gupta Omprakash 
Published: March 2024

AbstractLinguistic relativity (linguistic patterns influence perception and cognition) is crucial for marketing communication effectiveness. The argument is that linguistic relativity enablers in marketing communication may assist marketers, communicators, and creators achieve the desired “effectiveness” more successfully. Based on this premise, prior research in consumer studies has examined language's mediation role in consumer perception and cognition. However, the question of identifying these enablers and the plausible existence of any structural relationship among them persists. This article, therefore, seeks to examine the identification and interrelationship of linguistic relativity enablers in the context of social marketing communication. We examine the query through in‐depth interviews with specific respondents with a strong and influential relationship with the subject. This study presents the analysis conducted through Fuzzy‐ISM and Fuzzy‐MICMAC and presents relative findings by providing a structure with the potential to be used in longitudinal studies.
Feasibility of a novel atrial mechanical sensing method for leadless atrioventricular synchronous pacing
Authors: Kazawa Shuichiro , Kazawa Chifumi , Sakaida Tomoko , Murakami Hidetaka , Takada Yasuyuki , Kusume Takahiro , Terasawa Muryo , Tanaka Nobuhiro , Satomi Kazuhiro , Yazaki Yoshinao 
Published: March 2024

AbstractIntroductionFew studies have demonstrated a high degree of atrioventricular (AV) synchrony with a new leadless pacemaker called Micra AV. Our group previously reported a new and unique sensing method. We believe that this novel sensing method, “Simplified A3 method,” might facilitate better AV synchrony than a conventional sensing method in almost all cases with Micra AV implantation.MethodsWe conducted a non‐randomized retrospective study comparing the two pacing methods at two centers. From December 1, 2021 to October 31, 2022, Micra AV was implanted for 32 patients at the two centers. Twenty of the 32 patients with sinus rhythm and complete AV block were included in this study. In Group 1, the conventional setting was programmed as follows: auto A3 threshold, auto A3 window‐end (WE), and auto A4 threshold turned on during hospitalization. In Group 2, the “Simplified A3 method” was programmed as follows: auto A3 threshold and auto A3WE turned off. Instead, an intentionally prolonged A3WE (850–1000 ms) and low A3 threshold (A3 signal amplitude + 0.5–1.0 m/s2) were programmed.ResultsTwenty patients were analyzed. In Group 2, AV synchrony (%AMVp) was significantly higher at the first outpatient clinic (63.0 ± 5.7% vs 81.0 ± 4.2%, p = .03). A3 threshold was significantly lower in Group 2 (5.9 ± 0.7 m/s2, p < .05 vs 2.3 ± 0.5 m/s2, p < .05).ConclusionOur novel sensing method might be a more feasible sensing method for obtaining higher AV synchrony than the conventional algorithm.
Autosomal recessive non‐syndromic hearing loss genes in Pakistan during the previous three decades
Authors: Shadab Madiha , Abbasi Ansar Ahmed , Ejaz Ahsan , Ben‐Mahmoud Afif , Gupta Vijay , Kim Hyung‐Goo , Vona Barbara 
Published: March 2024

AbstractHearing loss is a clinically and genetically heterogeneous disorder, with over 148 genes and 170 loci associated with its pathogenesis. The spectrum and frequency of causal variants vary across different genetic ancestries and are more prevalent in populations that practice consanguineous marriages. Pakistan has a rich history of autosomal recessive gene discovery related to non‐syndromic hearing loss. Since the first linkage analysis with a Pakistani family that led to the mapping of the DFNB1 locus on chromosome 13, 51 genes associated with this disorder have been identified in this population. Among these, 13 of the most prevalent genes, namely CDH23, CIB2, CLDN14, GJB2, HGF, MARVELD2, MYO7A, MYO15A, MSRB3, OTOF, SLC26A4, TMC1 and TMPRSS3, account for more than half of all cases of profound hearing loss, while the prevalence of other genes is less than 2% individually. In this review, we discuss the most common autosomal recessive non‐syndromic hearing loss genes in Pakistani individuals as well as the genetic mapping and sequencing approaches used to discover them. Furthermore, we identified enriched gene ontology terms and common pathways involved in these 51 autosomal recessive non‐syndromic hearing loss genes to gain a better understanding of the underlying mechanisms. Establishing a molecular understanding of the disorder may aid in reducing its future prevalence by enabling timely diagnostics and genetic counselling, leading to more effective clinical management and treatments of hearing loss.
Integration of single‐cell and RNA‐seq data to explore the role of focal adhesion‐related genes in osteoporosis
Authors: Shi Xiaojian , Fu Qiang , Mao Jianyu , Yang Jiajie , Chen Ye , Lu Jiajia , Chen Aimin , Lu Nan 
Published: March 2024

AbstractIntegrin‐based focal adhesion is one of the major mechanosensory in osteocytes. The aim of this study was to mine the hub genes associated with focal adhesion and investigate their roles in osteoporosis based on the data of single‐cell RNA sequencing and RNA‐sequencing. Two hub genes (FAM129A and RNF24) with the same expression trend and AUC values greater than 0.7 in both GSE56815 and GSE56116 cohorts were uncovered. The nomogram was created to predict the risk of OP based on two hub genes. Subsequently, the competing endogenous RNA network was established based on two hub genes, 14 microRNAs and five long noncoding RNAs. Meanwhile, transcription factors‐hub gene network was established based on two hub genes and 14 TFs. Finally, 73 drugs were predicted, of which there were 13 drugs targeting FAM129A and 66 drugs targeting RNF24. In both mouse and human blood samples, FAM129A expression was decreased in granulocytes and RNF24 expression was increased in monocytes. In the mouse experiment, FAM129A and anti‐RNF24 were found to partially alleviate the progression of osteoporosis. In conclusion, two hub genes related to focal adhesion were identified by combined scRNA‐seq and RNA‐seq analyses, which might supply a new insight for the treatment and evaluation of OP.
Improvements of myocardial strain and work in diabetes patients with normal ejection fraction after empagliflozin treatment
Authors: Cheng Xueqing , Huang Peina , Liu Hongyun , Bi Xiaojun , Gao Yiping , Lu Ruirui , Gao Yipeng , Liu Yani , Deng Youbin 
Published: March 2024

ABSTRACTAims/IntroductionTo assess the effect of empagliflozin treatment on left ventricular (LV), right ventricular (RV) and left atrial (LA) functions in diabetes patients with normal ejection fraction.Materials and MethodsThe study included a total of 128 diabetes patients with multiple cardiovascular risk factors who were subjected to a 6‐month follow up from the initiation of empagliflozin treatment. Before and after treatment with empagliflozin, LV, RV and LA strain, and noninvasive myocardial work parameters were evaluated by speckle tracking echocardiography.ResultsIn 128 diabetes patients (mean age 56 ± 8 years, 85 men) with multiple cardiovascular risk factors, myocardial strain and work parameters were impaired, despite the absence of significant clinical symptoms of heart failure. After 6‐month treatment with empagliflozin, the absolute value of LV strain in all directions increased, represented by LV global longitudinal strain (−18.0 ± 1.7% to −19.2 ± 1.7% [mean ± SD]). The same trend in LV global work efficiency (93 [91–94] % to 94 [93–95] % [median (IQR)]), RV free‐wall longitudinal strain (−24.0 ± 2.7% to −25.0 ± 2.8%), LA reservoir (31 ± 5% to 34 ± 5%) and conduit strain (−14 ± 4% to −16 ± 4%) was also observed. LV mass index (106.9 ± 16.8–103.6 ± 16.4 g/m2) and LV global wasted work (143 [111–185] mmHg% to 108 [88–141] mmHg%) decreased after treatment (P < 0.05 for all). LV volume and LA volume index remained unchanged after treatment. In the multivariable analysis, the change in LA reservoir strain (β = 0.050, P = 0.035) and baseline global longitudinal strain (β = −0.488, P < 0.001) were independent predictors of improvement in LV global longitudinal strain.ConclusionsThis study suggests that 6‐month treatment with empagliflozin improved LV, RV and LA functions in diabetes patients with normal ejection fraction.
Clinical factors influencing the effectiveness of microplasma fractional radiofrequency treatment for atrophic acne scars: A retrospective analysis
Authors: Li Jing , Duan Fangfang , Kuang Jie , Liu Xiao , Wei Jia , Zhao Jinghui 
Published: March 2024

AbstractBackgroundMicroplasma fractional radiofrequency (MP FRF) technology has been increasingly used for acne scars. Nevertheless, little evidence has analyzed the factors influencing its effectiveness before and during treatment.AimsTo evaluate the clinical factors affecting the effectiveness of MP FRF therapy for atrophic acne scars.MethodsWe analyzed retrospectively the clinical data of 79 acne scar patients treated with MP FRF technology. The outcome of interest included the effectiveness and adverse events after MP FRF treatment. Multivariable logistic regression was utilized to evaluate clinical factors associated with effectiveness after the initial session.ResultsAll patients received 115 sessions of MP FRF therapy (average: 1.5 sessions). Twenty‐eight (35.4%) patients improved moderately to excellently after one session. We found that the severe grade before treatment was negatively correlated with the effectiveness according to Goodman–Baron qualitative scores (OR = 0.02, 95% CI [0.001, 0.37], p = 0.009). The presence of icepick scars was also a negative correlation factor for the effectiveness (OR = 0.06, 95% CI [0.004, 1.00], p = 0.049). Furthermore, after excluding the effects of icepick scars and Goodman–Baron scores before treatment, ECCA scores were also correlated with effectiveness (OR = 1.04, 95% CI [1.01, 1.06], p = 0.009).ConclusionMP FRF therapy was effective in treating atrophic acne scars with no permanent adverse events. The severity of Goodman–Baron qualitative scores and icepick scars were independent clinical factors affecting effectiveness, suggesting the possible requirement for additional treatments other than MP FRF for severe acne scars and icepick scars.
Inhibin A contributes to the tumorigenesis of oral squamous cell carcinoma by KIAA1429‐mediated m6A modification
Authors: Chen Xiaoqing , Fan Renxiu 
Published: March 2024

AbstractBackgroundInhibin A and N6‐methyladenosine methylation modifications participate in oral squamous cell carcinoma development. However, the N6‐methyladenosine modification of Inhibin A in oral squamous cell carcinoma has not been revealed. This study reveals a key gene “Inhibin A” that may affect the tumorigenesis of oral squamous cell carcinoma and its molecular mechanisms on N6‐methyladenosine methyltransferase KIAA1429‐mediated N6‐methyladenosine methylation modification.MethodsBioinformatics analysis and quantitative real‐time polymerase chain reaction identified the potential regulatory genes in oral squamous cell carcinoma. We examined the changes in the proliferation (Cell Counting Kit‐8 assay), migration (transwell migration assay), and invasion (transwell invasion assays) of oral squamous cell carcinoma cells. We performed a xenograft tumor experiment to validate the role of Inhibin A in oral squamous cell carcinoma in vivo. The interactions between Inhibin A and KIAA1429 were analyzed using bioinformatics, methylated RNA immunoprecipitation‐qPCR, quantitative real‐time polymerase chain reaction, and Western blotting experiments.ResultsInhibin A had the highest expression in patients with oral squamous cell carcinoma. Inhibin A silencing impaired the ability of oral squamous cell carcinoma cells to proliferate, migrate, and invade, as well as limited the tumorous growth of oral squamous cell carcinoma cells in vivo. Bioinformatics analysis showed that Inhibin A expression positively interacted with KIAA1429 expression in The Cancer Genome Atlas database. The levels were also upregulated in our clinical samples. Furthermore, KIAA1429 silencing repressed the N6‐methyladenosine level of Inhibin A in oral squamous cell carcinoma.ConclusionsInhibin A promotes the tumorigenesis of oral squamous cell carcinoma by KIAA1429‐mediated N6‐methyladenosine modification. This study adds to our current knowledge of the molecular mechanisms underlying oral squamous cell carcinoma malignancy.
Local information and the stabilization role of local government: Evidence from a natural experiment in China
Authors: Jia Junxue , Li Rong , Liu Chang , Ning Jing 
Published: March 2024

AbstractOates, (1972) argues that local governments cannot use conventional policies to stabilize economies, due to fiscal spillovers and beggar‐thy‐neighbor policies. Hayek's theory implies it is efficient for a central government to devolve decision‐making authority to local governments that have informational advantages. This paper tests the different theoretical implications by examining a natural experiment caused by the income‐tax‐sharing reform in China. Our analysis reveals that local government size does have a stabilization effect, but vertical fiscal imbalance (VFI) substantially weakens this stabilizing effect; lack of local information is the key factor leading to this influence of VFI.
Mitigating the definitional quagmire in innovation research: An inclusive definition of innovation as a template for defining various types of innovations uniformly
Authors: Varadarajan Rajan 
Published: March 2024

AbstractA review of research on innovation and new product development points to certain foundational issues of concern such as the use of (1) different definitions of constructs germane to the field (e.g., definitions of innovation, innovativeness, and specific types of innovations), (2) different construct labels to refer to specific innovation types, and (3) the same construct label to refer to different innovation types. Over the years, scholars have cautioned that a body of research in a field of study that is based on different definitions of constructs germane to the field would slow the advancement of knowledge. Against this backdrop, the purpose of this Catalyst paper is to initiate a conversation on the prospects for mitigating the definitional quagmire in research on innovation and new product development by developing an inclusive definition of innovation that can be used as a template for defining various types of innovations uniformly. Toward this end, the paper proposes an inclusive definition of innovation based on idea, outcome, and value creation as the defining or essential characteristics of all innovations. Definitions of 10 specific types of innovations defined in a consistent manner, employing the proposed inclusive definition of innovation as a template are also presented. All else being equal, consistency across research studies in the definitions of constructs employed can be conducive to the advancement of knowledge in a field of study in several ways such as streamlining the curation of the research‐based knowledge, facilitating the grounding of new research in prior research (i.e., curated knowledge), and eliminating differences across studies in the definitions of key constructs employed as a potential confound on the research findings.
Applying unoccupied aerial systems to assess genetic gain from mass selection for tembotrione tolerance in sorghum (Sorghum bicolor)
Authors: Crozier Daniel , Beechinor Kayla A. , Young Blake , Bagavathiannan Muthukumar , Rooney William L. 
Published: March 2024

AbstractThe post‐emergent herbicide options to control grasses in sorghum have historically been limited. Therefore, a mass selection program was implemented to increase the tolerance of sorghum to the broad‐spectrum herbicide tembotrione. The objectives of this study are to determine (i) which observation timing and vegetative indices are most effective in assessing sorghum injury to tembotrione using unoccupied aerial system imagery and (ii) if mass selection can be used to increase the tolerance of sorghum to tembotrione. Using an unoccupied aerial system, several vegetative indices, collected at either 14 or 21 days after tembotrione application, accurately measured sorghum injury. Over four cycles of selection, tembotrione tolerance increased in a linear and consistent manner. This demonstrates that mass selection can be used as a strategy for developing herbicide tolerance in crops. The modest, but steady genetic gain indicates both the quantitative nature of the trait and sufficient heritability to improve it. Given further development, tembotrione‐resistant sorghum hybrids could provide an effective means of post‐emergent weed control for a range of common weeds.
Co‐overexpression of SWEET sucrose transporters modulates sucrose synthesis and defence responses to enhance immunity against bacterial blight in rice
Authors: Singh Jitender , James Donald , Das Shubhashis , Patel Manish Kumar , Sutar Rashmi Ranjan , Achary V. Mohan Murali , Goel Naveen , Gupta Kapuganti Jagadis , Reddy Malireddy K. , Jha Gopaljee , Sonti Ramesh V. , Foyer Christine H. , Thakur Jitendra Kumar , Tripathy Baishnab C. 
Published: March 2024

AbstractEnhancing carbohydrate export from source to sink tissues is considered to be a realistic approach for improving photosynthetic efficiency and crop yield. The rice sucrose transporters OsSUT1, OsSWEET11a and OsSWEET14 contribute to sucrose phloem loading and seed filling. Crucially, Xanthomonas oryzae pv. oryzae (Xoo) infection in rice enhances the expression of OsSWEET11a and OsSWEET14 genes, and causes leaf blight. Here we show that co‐overexpression of OsSUT1, OsSWEET11a and OsSWEET14 in rice reduced sucrose synthesis and transport leading to lower growth and yield but reduced susceptibility to Xoo relative to controls. The immunity‐related hypersensitive response (HR) was enhanced in the transformed lines as indicated by the increased expression of defence genes, higher salicylic acid content and presence of HR lesions on the leaves. The results suggest that the increased expression of OsSWEET11a and OsSWEET14 in rice is perceived as a pathogen (Xoo) attack that triggers HR and results in constitutive activation of plant defences that are related to the signalling pathways of pathogen starvation. These findings provide a mechanistic basis for the trade‐off between plant growth and immunity because decreased susceptibility against Xoo compromised plant growth and yield.