bluuebunny/crossref_metadata_2025_split huggingface dataset:
Compound heterozygous PROC mutations cause lipedema in humans
Authors: Wang Jiqiu , Yang Ruikai , Ni Mengshan , Gu Weiqiong , Gu Bin , Zhang Juan , Zhu Yinmeng , Jia Yingying , Xie Zhiyao , Li Jiafu , Liu Chunye , Zhao Yuxiao , Yuan Bo , Bao Qiyuan , Xu Heng , Ding Qiurong , Wang Weiqing , Liu Ruixin , Ning Guang , Zeng Yi 
Published: February 2025

Abstract

Lipedema is a hereditary disorder characterized by excessive accumulation of subcutaneous adipose tissue in the limbs. The genetic causes and mechanisms underlying abnormal adipocyte expansion in lipedema, however, remain unknown. Here, we identify compound heterozygous mutations in the PROC gene in three lipedema patients from two unrelated consanguineous families. In vitro studies demonstrate the wild-type Protein C (PC), encoded by PROC, plays an inhibitory role in adipogenesis; conversely, the identified PC mutants, p.R271Q and p.R272H, fail to inhibit this process. In mice, the receptor of PC (PROCR) marks adipocyte progenitors, and conditional deletion of PROCR in these cells leads to an increased number of newborn adipocytes within white adipose tissue (WAT). Transcriptomic analysis alongside chemical blockage tests identifies HIF-1α as a primary downstream transcription factor mediating PC–PROCR signaling in adipogenesis. Furthermore, adipose biopsy samples from the patients’ thighs exhibit hyperplastic expansion of adipocytes, while single-nucleus RNA sequencing confirms increased adipogenic capacity and down-regulated HIF-1α activity in affected subjects. These findings establish PROC as the first causal gene for human lipedema and unveil a previously unexpected role of the PC–PROCR axis in orchestrating adipogenesis.
Ultrafast charge-transfer-induced spin transition in cobalt-tungstate molecular photomagnets
Authors: Ohkoshi Shin-ichi , Nakamura Kazuki , Nakabayashi Koji , Guérin Laurent , Privault Gaël , Herve Marius , Collet Eric 
Published: February 2025

Abstract

In photoinduced phase transition materials, where both charge transfer and spin transition occur, there has been a long debate on which of the two processes are leading the phase transition. Herein, we present experimental evidence supporting an optically charge-transfer-induced spin transition (CTIST) process, as demonstrated through femtosecond optical spectroscopy in two-dimensional cyanido-bridged cobalt-tungstate photomagnets. Optical and magnetic investigations reveal that the optical excitation of the ground low-temperature (LT) CoIIILS-WIV state drives a photoinduced phase transition towards the CoIIHS-WV state, similar to the high temperature (HT) state. Ultrafast spectroscopy further indicates that this optical excitation of the intermetallic W-to-Co charge-transfer band produces a transient photoexcited (PE) CoIILS-WV state, which decays within 130 fs through a spin transition towards the CoIIHS-WV state. Here we show that the CTIST dynamics corresponds to the CoIIILS-WIV (LT) → CoIILS-WV (PE) → CoIIHS-WV (HT) sequence. The present work sheds a new light on understanding optical dynamics underlying the photoinduced phase transitions.
Chronic Suppression of a Multidrug-Resistant Pseudomonas aeruginosa in Prosthetic Joint Infection Using Personalized Bacteriophage Treatment
Authors: Urish Kenneth , Arya Rekha , Sadaka Nadine , Frear Andrew , Rempuszewski Jewelia , Ma DongZhu , Knapick Beth , Parker Dana , Cooper Vaughn , Tyne Daria Van , Shah Neel , Doub James , Akbey Ümit , Fackler Joseph , Basu Subhendu , Garber Arkadiy 
Published: February 2025

Abstract

Multidrug resistant (MDR) bacterial infections without antibiotic options are a public health emergency. Infections associated with medical implants serve as an example. Conventional antibiotics have limited ability to eradicate these infections as they are associated with antibiotic-tolerant biofilms. Here, we report the use of bacteriophage therapy for the treatment of a MDR, non-operable Pseudomonas aeruginosa periprosthetic joint infection that had failed multiple antibiotic and surgical interventions. Treatment with intermittent bacteriophage therapy alone without antibiotics over a 2 year time period resulted in clinical resolution of the infection, but not microbiological eradication. Bacteriophage therapy established this control, in part, by altering bacterial virulence and disrupting biofilm. Whole genome sequencing demonstrated the continued presence of bacteriophage during treatment without integration into the bacteria genome. This provides preliminary evidence that bacteriophage can be used to treat multidrug-resistant infections when antibiotic options do not exist.
Assessment of Radon gas concentration in companies' warehouses and ventilation CFD modeling
Authors: Hassan Ramadan 
Published: February 2025

Abstract

Radon (Rn-222) is a naturally occurring radioactive gas that, when inhaled along with its decay products, can increase the risk of lung cancer. This study assessed potential radon exposure risks in warehouses by analyzing concentration levels and their relationship with ventilation conditions. Results revealed significant variations in radon concentrations across different locations, emphasizing the need for further investigation and mitigation measures. A warehouse room model was simulated using CFD in SimScale to analyze airflow and radon dispersion. The average radon concentration across the sampled sites was 27.68 Bq/m³, well below the WHO reference level of 100 Bq/m³ (2.7 pCi/L). However, poorly ventilated areas exhibited much higher levels, with Sample 18 reaching 68.7 Bq/m³, while well-ventilated areas, such as Sample 7, recorded only 12.6 Bq/m³. The mean annual effective dose (AED) was 0.175 mSv/year, within the ICRP’s recommended public exposure limit of 1 mSv/year. However, some poorly ventilated locations exceeded this average, with AED values reaching 0.433 mSv/year, indicating potential health risks. The estimated average lifetime lung cancer probability (CPPP) was 3.14 × 10⁻⁶. The study highlights how warehouse ventilation systems primarily circulate air at higher levels, neglecting lower zones where radon accumulates. Improving ventilation at lower levels is crucial to ensuring safer air quality and minimizing health risks for workers.
3D Spatiotemporal Activation Mapping of Cardiac Organoids Using Conformal Shell Microelectrode Arrays (MEAs)
Authors: Kim Deok-Ho , Choi Soo Jin , Liu Zhaoyu , Yang Feiyu , Wang Hanwen , George Derosh , Gracias David 
Published: February 2025

Abstract

Cardiac organoids have emerged as transformative models for investigating cardiogenesis and cardiac diseases. While traditional 2D microelectrode arrays (MEAs) have been used to assess the functionality of cardiac organoids, they are limited to electrophysiological measurements from a single plane and do not capture the 3D propagation of electrical signals. Here, we present a programmable, shape-adaptive shell MEA designed to map the electrical activity across the entire surface of cardiac organoids. These shell MEAs are fabricated on-chip, with tunable dimensions and electrode layout, enabling precise encapsulation of spherical organoids. Using shell MEAs, we generated 3D isochrone maps with conduction velocity vectors, revealing the speed and trajectory of electrical signal propagation in spontaneously beating cardiac organoids. The optical transparency of the shell MEAs allowed for simultaneous calcium imaging, validating the electrophysiological propagation pattern. To demonstrate their utility in cardiotoxicity screening, we monitored the electrophysiological changes of organoids treated with isoproterenol and E-4031 over nine days. We anticipate that shell MEAs, combined with spatiotemporal mapping, can significantly advance the development of spatially organized cardiac organoids, structural disease models, and high-throughput drug screening platforms.
Sustained Release of Dual p38 Inhibitors via Supramolecular Hydrogels to Enhance Cardiac Repair after MI/R Injury
Authors: Wang Di , Xu Aoxue , Su Haitao , Zhang Youpei , Jiang Lingling , Wang Yaguang , Liang Gaolin , Zhang Ye 
Published: February 2025

Abstract

Activation of p38 mitogen-activated protein kinase plays an important role in the progression of ventricular muscle inflammation after myocardial ischemia-reperfusion (MI/R). The inhibition of p38 activation in ischemic myocardium can reduce ventricular muscle remodeling post-MI. However, owing to the dynamic change of p38 in ischemic myocardium after MI, the clinical therapeutic effect of p38 inhibitors is insufficient. Herein, we describe the design of a hydrogelator Nap-Phe-Phe-Thr-Gly-Tyr-OH (Nap-TGY) to coassemble the p38 inhibitor SB202190 (SB), a p38 responsive supramolecular hydrogel (Gel Nap-TGY+SB) for local administration and p38 responsive release of SB to efficiently improve the inflammatory microenvironment. Under the overexpression of p38 in ischemic myocardium, Nap-TGY in the hydrogel is phosphorylated to yield hydrophilic Nap-Phe-Phe-Thr(H2PO3)-Gly-Tyr(H2PO3) (Nap-TpGYp), triggering the disassembly of the hydrogel and a responsive release of the inhibitor. Injection of hydrogel into the ischemic myocardium significantly reduces p38 phosphorylation, mitigates inflammation, and enhances angiogenesis. These findings suggest a novel therapeutic strategy for ischemic cardiomyopathy through modulation of the p38 mitogen-activated protein kinase (MAPK) pathway.
Development of a Foundation Fieldbus Access Equipment
Authors: DING Changfu , ZHANG Bo , LI Xiaowei , YANG Qingyu 
Published: February 2025

Abstract

In order realize the digitalization data transmission of 4 ~ 20mA signals and switch contact signals related to the control of electrical equipment in a large nuclear fuel chemical plant, a Foundation Fieldbus access equipment was developed. The development of the access equipment was carried out through overall design, principle design, and design of major components. Two independent microprocessors are designed to undertake the signal processing of electrical equipment and the signal processing of Foundation Fieldbus respectively by using embedded microprocessor technology to realize the fully digitalization data transmission and control of electrical equipment. By testing and experiments, the access equipment has high data transmission accuracy, and meets the requirements of the Foundation Fieldbus specification, and has good economic benefits for a large nuclear fuel chemical plant.
GSTLB: A Secure GPS-Enabled Software-Defined Network Leveraging Transfer Learning and Blockchain for Industrial IoT (IIoT)
Authors: BAGRUDEEN BAZEER AHAMED 
Published: February 2025

Abstract

IIoT sensors bridge the gap between industry and technology by assisting in intelligent automation of the sector. The proposed method uses GPS to track assets and identify products for customers. It also uses TL (Transfer Learning) to identify machine problems, which alerts for immediate service. Using the 5G network greatly facilitates faster communication and surveillance control. All of the data from these various applications, including GPS location tracking, surveillance, and alerting, are stored in a block chain where malicious data can be added with the original data or eves dropping is possible, which could interfere with the industry's normal operations. Plot-based features and CNN-based feature extraction are both used to get around this, and the network's efficiency and security are increased by this hybrid approach of separating dangerous input from the original data. When using the block chain methodology, data is compressed to make it unified because Network Slicing is the primary concept utilized in industry and SDN (Software Defined Network) is used as a centralized one for communication. According to the simulation results, the suggested methodology improves IIoT network security, speeds up data transmission, and conducts ongoing product and machine inspections, all of which significantly aid in expanding the supply chain.
Spatial and temporal patterns in water quality analysis near effluent dump-sites in Harare, Zimbabwe
Authors: Matengo Tafadzwa , Mwedzi Tongayi , Mbetu Jabulani 
Published: February 2025

ABSTRACT

Pollution of underground water can significantly contribute to health problems, leading to higher rates of morbidity and mortality. A study was conducted in six Harare suburbs (Glen View, Budiriro, Kuwadzana, Kambuzuma, Mabvuku, and Hatcliffe) to assess the quality of underground water, focusing on physical, chemical, and biological characteristics near effluent disposal farms (Ingwe and Crowborough). The areas were grouped by distance from the farms: near (&lt;5 km), far (5–15 km), and very far (&gt;15 km). The study assessed how the water quality changed with the seasons and distance from the farms. It also compared the water quality parameters with World Health Organization (WHO) standards. Water quality parameters of underground drinking water in Harare exhibited significant seasonal variations. The average of environmental and biological variables in Harare significantly deviated from WHO standards. Stronger correlations were found between water quality parameters and site-specific characteristics, rather than with seasonality or distance. No Shigella or Vibrio cholerae were found, but Escherichia coli was detected periodically at specific sites in Budiriro in the wet season and Kuwadzana in the dry season only. The study highlights the critical role of seasonal fluctuations and site-specific characteristics in shaping water quality, underscoring the need for tailored approaches to underground water management.
Forecasting monthly rainfall and temperature patterns in Van Province, Türkiye, using ARIMA and SARIMA models: a long-term climate analysis
Authors: Yavuz Veysel Süleyman 
Published: February 2025

ABSTRACT

This study investigates monthly rainfall and temperature trends in Van Province, Türkiye, using ARIMA and SARIMA models, with a dataset spanning from 1955 to 2023. The ARIMA(3,1,0) model for rainfall and ARIMA(0,1,1) model for temperature were selected based on Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC) values, achieving AIC scores of 788.224 and 172.077, respectively. To address seasonality, SARIMA models were also applied, with SARIMA(3,1,0)(2,1,0)[12] for rainfall and SARIMA(0,1,1)(2,1,0)[12] for temperature, yielding AIC scores of 672.061 and 163.669. Diagnostic tests, including the Ljung–Box and Jarque–Bera tests, confirmed model adequacy by indicating minimal autocorrelation and normal residual distributions. These models successfully captured seasonal and long-term patterns, offering valuable insights for regional planning in water resource management and agriculture. The study underscores the potential of ARIMA and SARIMA models for climate forecasting, with suggestions for future enhancements using hybrid approaches to improve predictions under non-linear conditions.
Yueju Pill Inhibits Apoptosis by Regulating the SCF/c-Kit/PI3K/AKT Signaling Pathway to Ameliorate Functional Dyspepsia
Authors: Gu Yaru , Biao Yaning , Liu Chenxu , Zhang Yufang , Gao Ya , Xue Yucong , Zhang Yixin 
Published: February 2025

Objective:
This study aimed to investigate the therapeutic effect of Yueju Pill (YJP)
on functional dyspepsia (FD) rats and its mechanism of promoting gastrointestinal motility.

Methods:
We replicated FD rat models using classical tail pinching and irregular feeding for 14
days. After 28 days of YJP treatment, we measured the gastric emptying rate and intestinal propulsion
rate of the rats. Hematoxylin-eosin (H&E) staining was used to observe the pathological
damage in the gastric antrum. The serum levels of related brain-gut peptides (BGPs) were determined
using the enzyme-linked immunosorbent assay (ELISA). Furthermore, we detected
the expression of proteins related to the SCF/c-Kit/PI3K/AKT signaling pathway through
Western blot and immunohistochemistry. Finally, we assessed the levels of apoptosis using the
TUNEL assay.

Results:
YJP improved gastric emptying and small intestine propulsion rates while reducing
gastric tissue injury in FD rats. Moreover, YJP increased the levels of gastrin (GAS) and
ghrelin (Ghrelin) and decreased the levels of cholecystokinin (CCK) and vasoactive intestinal
peptide (VIP). YJP also elevated the levels of SCF, c-kit and Bcl-2, promoted the phosphorylation
of PI3K and AKT, and inhibited the expression of Bax.

Conclusion:
YJP achieved the effect of FD treatment by regulating the SCF/c-Kit/PI3K/AKT
pathway, providing a theoretical basis for the clinical treatment of FD.
YY2 Serves as a Novel Prognostic Biomarker Correlated with Immune Microenvironment and Glycolysis in Esophageal Carcinoma
Authors: Gou Haimei , Yang Hui , Cheng Jiao , He Shuang , Luo Can , Chen Xin , Zhong Xiaowu 
Published: February 2025

Background:
Yin Yang 2 (YY2) plays a pivotal role in various tumorigenic processes;
however, its specific involvement in esophageal carcinoma (ESCA) remains elusive. This study
aims to investigate the expression and potential functional significance of YY2 in ESCA.

Methods:
The expression and functions of YY2 in ESCA were analyzed using a broad range of bioinformatics
databases and tools, including TCGA, TIMER, TISIDB, QUANTISEQ, cBioPortal,
DNMIVD, LinkedOmics, DAVID, GSEA, GEPIA2, LASSO, miRWalk, miRDB, and TargetScan.
Furthermore, RT-qPCR, immunohistochemical staining, western blot, CCK8 assay, and wound
healing assay were employed to validate the involvement of YY2 in ESCA pathogenesis.

Results:
Bioinformatics analyses revealed that the YY2 gene is upregulated in ESCA tissues, with its
high expression significantly associated with poor prognosis and elevated levels of M2 macrophages,
NK cells, Tregs, CTLA4, TIGIT, and Siglec-15. Validating the ESCA samples demonstrated that
knockdown of YY2 effectively inhibited cell proliferation and migration in ESCA cells. The biological
functions of YY2 and its co-expressed genes were primarily associated with transcriptional regulation,
DNA methylation, glycometabolism, and ubiquitination. Moreover, the regulatory network of YY2 in
the glycolysis pathway was found to involve multiple genes and miRNAs. Finally, a prognostic model
based on YY2 and its associated glycolysis genes revealed a strong inverse correlation between higher
risk scores and lower survival rates in esophageal adenocarcinoma (EAC).

Conclusion:
YY2 may serve as a promising prognostic biomarker and an innovative therapeutic
target for patients with ESCA, regulating cell proliferation, migration, immune microenvironment,
and glycolysis.
Harary Spectra and Energy of Certain Classes of Graphs
Authors: Ashoka Kuruba , Parvathalu Bolle , Arumugam Subramanian 
Published: February 2025

Aims:
To investigate the Η-eigenvalues and Η-energy of various types of graphs,
including κ-fold graphs, strong κ-fold graphs, and extended bipartite double graphs and establish relationships between the Η-energy of κ-fold and strong κ-fold graphs and the Η-energy of
the original graph G, we explore the connection between the Η-energy of extended bipartite
double graphs and their ordinary energy and find the graphs that share equienergetic properties
with respect to both the ordinary and Harary matrices.

Background:
The Η-eigenvalues of a graph G are the eigenvalues of its Harary matrix Η(G).
The Η-energy εΗ(G) of a graph, G is the sum of the absolute values of its Η-eigenvalues. Two
connected graphs are said to be Η-equienergetic if they have equal Η-energies. They are said to
A-equienergetic if they have equal A-energies. Adjacency and Harary matrices have applications in chemistry, such as finding total Π-electron energy, quantitative structure-property relationship (QSPR), etc.

Objective:
We determined the Η-spectra of κ-fold graphs, strong κ-fold graphs and extended
bipartite double graphs and established connections between the Η-energy of different types of
graphs and their original graph G for investigating the relationship between the Η-energy of
extended bipartite double graphs and their ordinary energy and the graphs that share equienergetic properties with respect to both the adjacency and Harary matrices.

Methods:
Spectral algebraic techniques are used to calculate the Η-eigenvalues and Η-energy
for each type of graph and compare the Η-energies of different graphs to identify the equienergetic properties and derive relationships between the Η-energy of extended double cover graphs
and their ordinary energy.

Results:
We determined the Η-spectra of κ-fold graphs, strong κ-fold graphs and extended bipartite double graphs and established relationships between the Η-energy of κ-fold and strong
κ-fold graphs and the Η-energy of the original graph G. Then, we explored the connection between the Η-energy of extended bipartite double graphs and their ordinary energy and presented
graphs demonstrating equienergetic properties concerning both adjacency and Harary matrices.

Conclusion:
The study provides insights into the Η-eigenvalues, Η-energy and equienergetic
properties of various types of graphs. The established relationships and connections contribute
to a deeper understanding of graph spectra and energy properties and the findings enhance the
theoretical framework for analyzing equienergetic graphs and their spectral properties.

Scope:
Possible extensions of this research could include investigating additional types of
graphs and exploring further explicit connections between different graph energies and spectral
properties.
Harary matrices are distance-based matrices, which can model distances between atoms in molecular structures and could be useful in organic synthesis to predict how molecular structures
behave.
Investigation of Norfloxacin Adsorption and Detection in Wastewater Utilizing Hyaluronic Acid and Dopamine-modified Mesoporous Carbon
Authors: Ge Yanhui , Liu Yubo 
Published: February 2025

Objective:
The increasing environmental pollution from antibiotics poses a significant
threat to public health, and this is a critical issue that requires immediate attention.

Methods:
In this study, a simple and effective surface modification technique was presented using
hyaluronic acid-dopamine conjugate (HA-DA) to impart anti-biofouling properties to basin-concave
mesoporous carbon (BCMC). The synthesized materials were characterized by scanning electron
microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR)
spectroscopy, nitrogen physisorption, and thermogravimetric analysis (TGA). The optimum adsorption
performance of the synthesized materials was investigated via adsorption isotherms and kinetics
experiments. Afterward, the obtained particles were used as solid-phase extraction subjects for Norfloxacin
(Nor) analysis in wastewater.

Results:
After being quantified by high-performance liquid chromatography (SPE-HPLC), the synthesized
BCMC@HA-DA demonstrated an impressive binding capacity of 14.80 mg/g for Nor. Following
six adsorption-desorption cycles, the adsorption revercory remained at 81.65%.

Conclusion:
The prepared BCMC@HA-DA could successfully be concentrated Nor from
wastewater, indicating significant potential for addressing environmental biofouling issues.