bluuebunny/crossref_metadata_2025_split huggingface dataset:
The circular RNA expression landscape across tissues implicates their functional roles in the marine gastropod Rapana venosa
Authors: Hong Mingwei , Huang Zixia 
Published: April 2024

Abstract

The carnivorous marine gastropod, Rapana venosa, also known as the veined rapa whelk, has recently gained considerable attention due to its dual significance as both a valuable commercial seafood resource and a biological invader with widespread ecological implications. Significant efforts have been made to investigate the molecular pathways governing the specific adaptations in R. venosa. However, the regulatory mechanisms of these pathways remain largely unclear. Circular RNA (circRNA), a newly-recognized class of non-coding molecules, plays crucial roles in post-transcriptional regulation, impacting various fundamental bioprocesses including development, cell proliferation, immunity, and disease pathogenesis. In this study, we conducted a comprehensive genome-wide profiling of circRNAs across eleven tissues of R. venosa. We identified a total of 1,214 circRNA genes across tissues, with 640 regarded as high-confidence candidates. CircRNAs displayed overall low expression levels, diverse isoform types, and dynamic expression patterns across various tissues. Our comparative analyses revealed a few circRNA genes with a great diversity of isoforms and abundant expression in the salivary gland, suggesting potential roles in the feeding process of R. venosa. Furthermore, Gene Ontology enrichment analysis indicated the potential involvement of commonly expressed circRNAs in fundamental cellular processes, including cell division, amide biosynthesis, and cellular response to hormone stimuli. Our study represents the initial in-depth examination of circRNAs across various tissues of R. venosa, laying the groundwork for future investigations into the molecular mechanisms that shape the unique biology, ecology, and behaviour of the marine gastropods.
Three-dimensional printing-guided percutaneous transcatheter replacement in coarctation of the aorta: a retrospective study
Authors: Xu Chennian , Liu Yang , Zhai Mengen , Jin Ping , Tian Zhiyuan , Yang Jian 
Published: April 2024

Abstract

Background
To evaluate the feasibility, effectiveness and assistant effect of 3D printed aortic model in the treatment on congenital coarctation of the aorta (CoA) in adolescents and adults.
Methods
From December 2018 to December 2023, a total of 10 patients with congenital coarctation of aorta underwent percutaneous balloon dilatation covered stent implantation in cardiovascular surgery of Xijing Hospital were retrospectively analyzed. There were 6 males and 4 females whose average age was (27.68 ± 13.45) years. One case complicated with ventricular septal defect (VSD). CT data of aorta were collected before operation, and the aorta was reconstructed by Mimics software and printed in 3D. The operation simulation was performed before operation to determine the best operation plan. Collect and analyze the clinical data of patients during hospitalization and follow-up.
Results
All the patients of CoA were successfully treated with percutaneous balloon dilatation covered stent implantation. The narrowest average diameter of CoA increased from (4.35 ± 2.61) mm before operation to (16.84 ± 1.99) mm immediately after operation (P < 0.05). The mean transconstrictive systolic pressure difference decreased from (81.29 ± 18.72) mmHg before operation to (15.52 ± 7.47) mmHg after operation (P < 0.05). The mean systolic blood pressure of the right upper limb decreased from (182.05 ± 38.99) mmHg preoperatively to (141.95 ± 32.11) mmHg postoperatively (P < 0.05). The mean systolic blood pressure of the lower limb increased from (121.52 ± 27.84) mmHg preoperatively to (131.81 ± 32.39) mmHg postoperatively (P < 0.05). Two patients with PDA and VSD underwent interventional occlusion at the same time without shunt. During the period of hospitalization and follow-up, there were significant cardiovascular complications.
Conclusions
Percutaneous balloon dilatation covered stent implantation is effective in the treatment of adolescents and adults with CoA in the short and medium term, with fewer complications, and the long-term effect needs furthshdie1er study.
Identification of Araucaria angustifolia trees in an urban forest fragment using UAV images and YOLOv7 structure
Authors: Correa Alan D'Oliveira , Prandini Matheus Kopp , Cysneiros Vinicius Costa , Pelissari Allan Libanio 
Published: April 2024

Abstract

This study addresses the identification of individuals of the Araucaria angustifolia species in urban forest fragments, specifically in the Mixed Ombrophilous Forest (FOM) in Curitiba, Paraná, Brazil. The aim of the study is to use UAV images and the computer vision technique of the YOLOv7 model to detect individuals of A. angustifolia. The FOM is essential for local biodiversity conservation and human well-being but faces challenges due to urban sprawl and the conversion of land use to agriculture. The species is critically endangered, requiring actions and strategies for its conservation. The study highlights the role of Unmanned Aerial Vehicles (UAVs) and deep learning techniques, such as Convolutional Neural Networks (CNNs), in identifying tree species in urban ecosystems. YOLOv7, an architecture based on CNNs, was chosen because of its detection capacity. YOLOv7 is especially effective at detecting a wide variety of objects, including people, vehicles, animals, household objects, road signs and much more, making it an ideal choice for identifying tree species in urban environments. The data was obtained by a DJI Mavic 3 UAV. Utilizing a UAV, the study area of the urban forest was flown over, generating an orthomosaic that was subsequently divided into 14 parts for training, validation, and testing. The YOLOv7 model was trained with the images to detect A. angustifolia trees present in the area. The results show that model achieved a precision of 79.3%, recall of 86.8%, and Mean Average Precision of 87% during training. Comparative analysis with forest inventory data reveals promising performance in detecting A. angustifolia trees. The average confidence of the model's classification was 76.18 ± 12.88%, with 80.81% being the most frequent classification for the median result. The present study uses the effective integration of UAV technology, YOLOv7 model with deep learning technique to detect and assess tree species in urban ecosystems. This approach provides an important tool for conservation strategies aimed at assessing and managing the tree biodiversity in urban forest remnants.
Impact of hemispherotomy on quality of life and burden of caregivers in children and adolescents: a retrospective observational study.
Authors: Daniel Bruna Frizzera , Leal Suenia Timotheo Figueiredo , Sakamoto Americo Ceiki , Thome Ursula , Machado Helio Rubens , Santos Marcelo Volpon , Santos Antonio Carlos , Hamad Ana Paula Andrade 
Published: April 2024

Abstract

Purpose: To evaluate QOL and caregiver burden of children and teenagers submitted to hemispherotomy for pharmacoresistant epilepsy, by comparing pre and post surgical intervention data.
Materials and methods: Retrospective analysis of pediatric patients submitted to surgical hemispherotomy before intervention (preOP) and their follow-up at 6 months (6M PO) and 2 years (2Y PO) after surgery. QOL was evaluated through the Quality of Life in Childhood Epilepsy (QVCE-50) questionnaire and caregiver burden, through the Zarit Burden Interview (ZBI) tool.
Results: Twenty-two patients were included in the study. Sixteen patients (72%) were classified as Engel I at 2Y PO follow-up. QVCE-50 scale showed improvement of total QOL at 2Y PO. In relation to QVCE-50 specific domains, there was an improvement in the physical domain and in the cognitive-education; a decrease in psychological and a stabilization in social/familiar domain scores. The majority of caregivers classified their burden as mild to moderate, with no PO improvement.
Conclusions: Hemispherotomy represents an effective seizure control treatment, as well as it contributes to improvement of QOL, particularly in the physical domain and in spite of children's physical and cognitive limitations. However, no improvement in caregiver burden was observed, probably due to the chronic condition of these patients, which might be worsened by social issues.
Student and lecturer perceptions of the use of an AI to improve communication skills in healthcare: an interview study
Authors: Brodowski Hanna , Dammermann Anna , Kinyara Muriel Marieke , Obst Martina Andrea , Samek Fabian , Peifer Corinna , Roese Katharina 
Published: April 2024

Abstract

Background and objectives Communication is a complex skill and key component for professionals in health care. Complex skills are best learned through experimental learning like role plays or simulated patient encounters. The aim of the present study is to explore how students and lecturers assess the conditions under which the use of an AI-based feedback system can promote the learning process.
Methods An interview study with health profession students and lecturers was conducted using a qualitative descriptive design. Recorded audio data was transcribed and evaluated by structuring qualitative content analysis using deductive and inductive coding. The research process was conducted and continually reflected by an interprofessional research team. Ethical approval was obtained.
Results Using qualitative content analysis, four major themes were identified. These are “conditions”, “communication scenarios”, “AI-based learning platform” and “debriefing”. Lecturers and students welcome the idea of AI providing feedback on verbal and para-verbal aspects. To implement AI-based feedback into a teaching programme AI functionality should be adaptable to the specific situation. Lecturers and students highlighted that AI could be particularly valuable for speech qualities which are often difficult for humans to assess. AI could give freedom to focus on additional aspects of the conversation by documenting desirable speech qualities. Lecturers and students prefer for the AI-based feedback to be given at the end of rather than within the role play. Furthermore, they wish for communication scenarios to be analysed repeatedly in order to track progress.
Conclusion Practicing in a safe environment and receiving competent credible feedback, with lecturers trained in facilitation, is a prerequisite for the entire learning progress.The integration of an AI-based feedback system should be characterised by both flexibility of the AI application and standardisation of the communication scenario.
Comparison of complications associated with diverting stoma after pouch surgery for ulcerative colitis or anterior resection for rectal cancer
Authors: Yoshimura Kosuke , Ohge Hiroki , Watadani Yusuke , Uegami Shinnosuke , Nakashima Ikki , Shimbara Kensuke , Doi Hirofumi , Kitagawa Hiroki , Takahashi Shinya 
Published: April 2024

Abstract

Background: The standard surgical procedure for ulcerative colitis (UC), including UC-associated cancer (UCAC), is total proctocolectomy (TPC) with mucosal resection and ileal-pouch anal anastomosis (IPAA). TPC and IPAA complications should be considered during the perioperative period. This study aimed to clarify problems associated with diverting loop stoma in TPC and IPAA based on complications during stoma construction and perioperative results of stoma closure.
Methods:Thirty-two patients who underwent TPC and IPAA with diverting loop ileostomy for ulcerative colitis (TPC group) and 26 patients who underwent concurrent low anterior resection with diverting loop ileostomy for lower rectal cancer (LAR group) were included. We retrospectively evaluated complications during stoma construction and the perioperative results of stoma closure between the two groups.
Results:The height of the oral side of the stoma was lower in the TPC group than in the LAR group. Stoma-related complications were particularly common. In stoma closure, the TPC group had a higher rate of intestinal resection and a later postoperative resumption of food intake. There were no significant differences in the incidence of Clavien-Dindo Grade 2 or higher complications or length of hospital stay.
Conclusions:Diverting loop stomas constructed withTPC and IPAA had a higher frequency of stoma-related complications and required intestinal resection for stoma closure. With appropriate surgical procedure and postoperative management, the postoperative outcomes were comparable to those of stoma closure in LAR.
Follicular helper and regulatory T cells in bladder cancer draining lymph nodes: clinical and prognostic significance
Authors: Mansourabadi Zahra , Ariafar Ali , Chenari Nooshafarin , Hakimellahi Hossein , Vahidi Yasmin , Faghih Zahra 
Published: April 2024

Abstract

Introduction:
Follicular helper and regulatory T cells (Tfh/TFR) cells are distinct subsets of CD4+ cells that have been recognized for their critical role in regulating cellular reactions within the germinal centers of lymphoid follicles. In the present study, we aimed to determine the presence and the frequency of these cells in draining lymph nodes of patients with bladder cancer (BC).
Methods
Forty-six patients with BC who had undergone radical cystectomy and pelvic lymph node dissection were enrolled. Following routine pathological examination, a portion of the dissected lymph nodes was minced to obtain a single-cell suspension. Mononuclear cells were then separated using Ficoll-Hypaque gradient centrifugation, and the samples with proper viability (> 95%) were subjected to further analysis. To phenotype the follicular subsets, cells were stained with appropriate fluorochrome-conjugated antibodies specific for CD4, CXCR5, BCL6, and FOXP3. The cells were then acquired on a four-color flow cytometer. The data were analyzed with the FlowJo software version 10.8.1 package.
Results
Our analysis indicated that, on average 37.89 ± 16.36% of CD4+ lymphocytes in draining lymph nodes of patients with BC expressed CXCR5. Most of them (28.73 ± 13.66) were negative for FOXP3, representing helper subsets. A small percent simultaneously expressed BCL6 transcription factor (1.65% ± 1.35), designated as Tfh (CD4+BCL6+CXCR5+FOXP3−). While less than 10% of CD4+ lymphocytes expressed CXCR5 and FOXP3, 1.78 ± 2.54 were also positive for BCL6, known as TFR. Statistical analysis revealed that the frequency of both Tfh and TFR cells was higher in draining lymph nodes of patients with tumor-infiltrated nodes (P = 0.035 and P = 0.079, respectively) compared to those with negative ones. The percentage of these cells (P = 0.031 for both) was also higher in high-grade tumors compared to low-grade ones.
Conclusion
Our data collectively indicated that, however approximately one third of CD4+ lymphocytes expressed CXCR5 and accordingly had the capacity to enter the follicles, less than 2% of them represented Tfh and TFR phenotypes. The percentage of these cells increased in progressed tumors and showed an association with negative prognostic factors.
Tax Collection and Management Digitalization and Enterprises’ Social Security Compliance
Authors: Yu Changlin , Li Yanming 
Published: April 2024

Abstract

Using the enforcement of the “Golden Tax Project Phase III” (Hereinafter referred to as “GTPIII”) as a quasi-natural experiment, this paper employs the Time-varying difference-in-difference (DID) method to comprehensively assess the impact of tax collection and management digitalization on enterprises’ social security compliance, based on National Tax Survey Data from 2007 to 2016 in China. This study found that the “GTPIII” significantly improved enterprises’ actual contribution rate of social security by 6.28 % and their likelihood of participating in social security by 17.62 % on average. Similar positive effects were obtained when various kinds of social security compliance behavior were considered. The “GTPIII” achieved such facilitating effects by promoting the interconnection of tax-related information between departments, discouraging enterprises from concealing contribution bases, and discouraging local governments’ flexible collection behavior. Heterogeneity research reveals that the “GTPIII” has a greater impact on social security compliance in SMEs, non-state-owned enterprises, low average salary and low profitability enterprises. Further analysis reveals that the “GTPIII”, while significantly increasing revenue for the regional social security fund, has caused enterprises to shift the tax burden by reducing jobs and lowering employee wages, forcing them to take the initiative to increase R&D expenditures.
Prevalence of Anxiety and Depressive Symptoms Among Clinical Physiotherapy Students Following Prolonged Lecturers Strike in a Sub-Saharan University.
Authors: Okonkwo Uchenna Prosper , Udechukwu Ray-Thelma Chisonyerem , Chukwuemeka Uchechukwu Martha , Amaechi Ifeoma Adaigwe , Akobundu Uzoamaka Nwakego 
Published: April 2024

Abstract
Objectives
To determine the prevalence of anxiety, and depressive symptoms among clinical physiotherapy students of Nnamdi Azikiwe University, Nnewi Campus, following prolonged lecturers (teachers) strike. a
Methods
This cross-sectional survey consecutively recruited 131 physiotherapy undergraduates of Nnamdi Azikiwe University, Nnewi Campus, in clinical classes. The researchinstruments were a socio-demographic questionnaire and the Hospital Anxiety and Depression Scale (HADS). Data was analyzed using descriptive statistics and a Chi-square inferential statistical test at an alpha level of 0.05.
Results: The majority of the participants were between 18-23 years of age, 75 (57.25%) of the participants were in their fourth year of study (400 level), while 56 (42.75%) of the participants were in their fifth year of study (500 level). The prevalence of anxiety and depression within the population of the study were 20.6% and 4.6% respectively. There was a significant association between the pairs of anxiety symptoms and gender (p=0.017); depressive symptoms and gender (p=0.008), with members of the male gender presenting more significant cases of anxiety and depressive symptoms.
Conclusion
The prevalence of depression and anxiety within the population of study were relatively high, and actively associated with gender.
What are community perspectives regarding brain- computer interfaces? A cross-sectional study of community-dwelling adults in the UK
Authors: El-Osta Austen , Ammouri Mahmoud Al , Khan Shujhat , Altalib Sami , Karki Manisha , Riboli-Sasco Eva , Majeed Azeem Majee 
Published: April 2024

Abstract
Background
Brain-computer interfaces (BCIs) have emerged as ground-breaking tools in neuroscience, enabling direct communication between the brain and external devices. This technology holds promise for enhancing the lives of individuals with neurological disorders offering new avenues for rehabilitation, communication and personal autonomy. Despite rapid technological advancements, public perceptions and ethical considerations surrounding BCI technology remain largely unexplored especially within the community setting in the UK.
Aims
The primary aim was to investigate the knowledge, attitudes and perceptions of community-dwelling adults in the UK regarding BCIs. We also sought to uncover ethical considerations and gauge public interest in potential medical and non-medical applications.
Methods
This cross-sectional study collected data from 846 respondents using a structured 29-item online questionnaire disseminated through various platforms. Data were collected on demographics, awareness of BCI technology, its applications and participants' willingness to use BCIs under different circumstances.
Results
Most respondents had never heard of or used BCIs, showing a significant awareness gap. Despite this, there was a strong interest in the medical applications of BCIs particularly for rehabilitation and aiding individuals with disabilities reflecting a cautious optimism towards their development. Conversely, non-medical uses elicited concerns about privacy, data security and long-term societal impacts highlighting ethical and regulatory challenges.
Conclusion
We recorded broad support for the development of BCI technology especially for applications that improve the quality of life for individuals with disabilities. This support was tempered by a pressing need for public education, engagement and the development of robust ethical guidelines and regulatory frameworks to navigate the future integration of BCIs into society. Addressing these challenges is crucial for realizing the full potential of BCIs in a manner that aligns with societal values and expectations.
Evaluation of the relationship between drugs’ safety competence and Medication Administration Error Perceptions in Clinical nurses of Northwest Iran
Authors: Zadeh Mehdi Mahmoud , Zia Sana Khalilzadeh , Dadkhah Behrouz , Mohamadi Mohammad Ail 
Published: April 2024

Abstract

Aims: The present study aimed to Evaluate of relationship between drugs’ safety competence and Medication Administration Error Perceptions in clinical nurses who are working in clinical nurses of northwest Iran
Background: The first and most important aim of a medical facility, as in the Nightingale pledge, is to not harm patients and protect their safety in the first place. One of the major threats to patient safety is drug-related errors.
Methods: This correlational study was conducted in Ardabil, northwest of Iran, in 2023. In these 300 nurses have been categorized by a proportional stratified sampling method. For data collection, we used the Bijani et al(2023) Persian version of the medication safety competence Questionnaire and the Wakefield et all (2005) Medication Administration Error Perceptions Questionnaire”.The collected data were analyzed in SPSS software (26) using descriptive statistics, independent t-test, analysis of variance, Pearson correlation coefficients, and linear regression model.
Results: The results revealed that the drug safety competencies in Nurses (77%) and Medication Administration Error Perceptions in nurses (96%) were at a moderate level. The mean nurses’ drug safety competence and Medication Administration Error Perceptions were reported (294.22 ± 26.61) and (135.48 ± 15.14) and this difference was statistically significant (P<0.001). There was a positive and significant correlation between drug safety competencies and Medication Administration Error Perceptions (P=0.007, r=0.15). A simple linear regression model showed that The field of "patient-centered medication management" of medication safety competency was a stronger predictor than other variables in medication error perception.
Conclusion: In the present study, nurses reported medication safety competence and perception of medication error at an average level. It is suggested that health policymakers and decision-makers take the necessary measures by holding consensus meetings and effective workshops to reduce medication errors and improve nurses' medication perception.