bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the relationship between farmland biodiversity and land-use intensity in Europe
Authors: Kleijn D , Kohler F , Báldi A , Batáry P , Concepción E.D , Clough Y , Díaz M , Gabriel D , Holzschuh A , Knop E , Kovács A , Marshall E.J.P , Tscharntke T , Verhulst J 
Published: November 2008

Worldwide agriculture is one of the main drivers of biodiversity decline. Effective conservation strategies depend on the type of relationship between biodiversity and land-use intensity, but to date the shape of this relationship is unknown. We linked plant species richness with nitrogen (N) input as an indicator of land-use intensity on 130 grasslands and 141 arable fields in six European countries. Using Poisson regression, we found that plant species richness was significantly negatively related to N input on both field types after the effects of confounding environmental factors had been accounted for. Subsequent analyses showed that exponentially declining relationships provided a better fit than linear or unimodal relationships and that this was largely the result of the response of rare species (relative cover less than 1%). Our results indicate that conservation benefits are disproportionally more costly on high-intensity than on low-intensity farmland. For example, reducing N inputs from 75 to 0 and 400 to 60 kg ha
−1
yr
−1
resulted in about the same estimated species gain for arable plants. Conservation initiatives are most (cost-)effective if they are preferentially implemented in extensively farmed areas that still support high levels of biodiversity.
Differential effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases
Authors: Sugiura Daisuke , Aida Satoshi , Denda‐Nagai Kaori , Takeda Kazuyoshi , Kamata‐Sakurai Mika , Yagita Hideo , Irimura Tatsuro 
Published: November 2008

The effects of MUC1 DNA vaccination on the orthotopic growth and liver metastasis of colon carcinoma cells were investigated in mice. Vaccination with MUC1 DNA resulted in immune responses that were effective in suppressing mouse colon carcinoma cells transfected with MUC1 cDNA. CD4+ T cells but not CD8+ T cells mediated this antitumor response as shown by the in vivo depletion of lymphocyte subpopulations with the use of anti‐CD4 or anti‐CD8 antibody. The effects of neutralizing antibodies in vivo revealed that the predominant effector molecule in preventing orthotopic tumor growth was FasL, whereas the effector molecule effective in preventing liver metastasis was tumor necrosis factor‐α. Colon carcinoma cells isolated from tumors growing in the ceca, spleens, and livers were shown to be equally sensitive to FasL and tumor necrosis factor‐α. The results strongly suggest that elimination of tumor cells initiated by DNA vaccination in the present protocol is mediated by antigen‐specific CD4+ T cells and the effector mechanisms in the cecum and in the liver are distinct due to a unique organ microenvironment. (Cancer Sci 2008; 99: 2477–2484)
Interferon‐α enhances CD317 expression and the antitumor activity of anti‐CD317 monoclonal antibody in renal cell carcinoma xenograft models
Authors: Kawai Shigeto , Azuma Yumiko , Fujii Etsuko , Furugaki Kou , Ozaki Shuji , Matsumoto Toshio , Kosaka Masaaki , Yamada‐Okabe Hisafumi 
Published: November 2008

A murine (mAHM) and a humanized (AHM) monoclonal antibody against CD317 (also called tetherin, BST2, or HM1.24 antigen), expressed preferentially in neoplastic B cells such as multiple myeloma, exhibited antitumor effects as a result of antibody‐dependent cellular cytotoxicity (ADCC). The putative interferon (IFN) response elements IRF‐1/2 and ISGF3 are present in the promoter of the CD317 gene, and IFN has been used for the treatment of not only myeloproliferative diseases but also solid tumors such as renal cell carcinoma (RCC) and melanoma. Therefore, we examined the effects of IFN on the expression of CD317 and on the antitumor activity of AHM and mAHM in RCC and melanoma. Flow cytometry and in vitro ADCC assays with human or mouse effector cells demonstrated that IFN‐α markedly increased the amount of cell surface CD317 and augmented the ADCC activity of mAHM and AHM in RCC cells and to a lesser extent in melanoma cells. Administration of IFN‐α to mice bearing RCC xenografts also increased the expression of CD317 in tumor cells. When coadministered with IFN‐α, mAHM exhibited more profound antitumor activity in both IFN‐α‐sensitive and ‐insensitive RCC xenograft models. Thus, AHM in combination with IFN‐α may be an effective therapy for the treatment of RCC. (Cancer Sci 2008; 99: 2461–2466)
Improvement in predicting tumorigenic phenotype of androgen‐insensitive human LNCaP prostatic cancer cell subline in recombination with rat urogenital sinus mesenchyme
Authors: Kanai Masahiro , Ishii Kenichiro , Kanda Hideki , Ogura Yuji , Kise Hideaki , Arima Kiminobu , Sugimura Yoshiki 
Published: November 2008

Hormone‐refractory prostate cancer, a heterogeneous disease, has varying degrees of androgen sensitivity. To understand the physiological changes in the hormone‐refractory state, the present study used a lineage‐derived androgen receptor (AR)‐positive, androgen‐insensitive prostate cancer cell line and evaluated the tumorigenic phenotype, focusing on tumor–stromal interactions in vivo. First, tumorigenic differences of cancer cells alone were examined in an androgen‐insensitive AR‐positive LNCaP subline, AIDL, compared with those of the androgen‐sensitive AR‐positive parental LNCaP and the androgen‐insensitive AR‐negative PC‐3 cells transplanted into subcutaneous, sub‐renal and prostatic orthotopic graft sites. Next, cancer cells were recombined with rat urogenital sinus mesenchyme (rUGM) to simulate the tumor‐stromal microenvironment. Tumors of AIDL and LNCaP without stromal components both formed well‐defined globular tumors and contained large blood‐filled areas, with no significant difference in tumor growth or histopathology regardless of the cell line's androgen sensitivity or graft site. In contrast, tumors of AIDL and LNCaP recombined with rUGM both showed reduction of blood‐filled areas in the tumors and increased tumor growth compared with cancer cells alone. Tumors of AIDL + rUGM recombinants were approximately three times as large as those of LNCaP + rUGM recombinants, whereas tumors of AIDL and LNCaP without rUGM were not different in size. In addition to the tumor size, cell proliferation (Ki‐67 labeling index) in tumors of AIDL + rUGM recombinants was significantly higher than that in tumors of LNCaP + rUGM recombinants. Immunoreactivities of AR, E‐cadherin and β‐catenin were decreased in AIDL + rUGM recombinants relative to AIDL alone and LNCaP + rUGM recombinants. These results demonstrated that tumorigenic features of androgen‐insensitive AR‐positive prostate cancer cells could be significantly influenced by rUGM. Therefore, this in vivo recombination model with rUGM may be useful in developing new treatment strategies. (Cancer Sci 2008; 99: 2435–2443)
ABCG2 Q141K polymorphism is associated with chemotherapy‐induced diarrhea in patients with diffuse large B‐cell lymphoma who received frontline rituximab plus cyclophosphamide/doxorubicin/ vincristine/prednisone chemotherapy
Authors: Kim In‐Suk , Kim Hoon‐Gu , Kim Dong Chul , Eom Hyeon‐Seok , Kong Sun‐Young , Shin Ho‐Jin , Hwang Sang‐Hyun , Lee Eun‐Yup , Lee Gyeong‐Won 
Published: November 2008

ATP‐binding cassette transporter G2 (ABCG2) is the most recently described transporter of the multidrug‐resistance pump and it promotes resistance to anticancer drugs such as doxorubicin, mitoxantrone, topotecan, and SN‐38. Of the ABCG2 polymorphisms, V12M and Q141K alter the functional activity of the ABCG2 transporter and influence the drug response and various toxicities to chemotherapeutic agents. We therefore evaluated the impact of the ABCG2 V12M and Q141K polymorphisms on the therapeutic outcomes and toxicities of primary rituximab plus cyclophosphamide/doxorubicin/vincristine/prednisone (R‐CHOP) therapy in 145 Korean patients with diffuse large B‐cell lymphoma (DLBCL). ABCG2 V12M and Q141K genotyping was carried out by pyrosequencing of polymerase chain reaction products. The clinical characteristics, treatment outcomes, toxicities of the patients, and the predictive value of the polymorphisms on response, survival, and adverse events to R‐CHOP for 145 patients were analyzed according to the ABCG2 V12M and Q141K polymorphisms. No differences were observed according to ABCG2 Q141K and V12M genotype in patient characteristics, disease characteristics, response, survival, or hematology toxicity profiles in patients with DLBCL who received frontline R‐CHOP chemotherapy. On multivariate analysis, grade I–IV diarrhea was statistically significant according to ABCG2 Q141K polymorphism (the QQ genotype vs the QK or KK genotypes; hazard ratio 2.835; 95% confidence interval 1.432–5.613; P = 0.003). This study demonstrates that the ABCG2 Q141K polymorphism may correlate with chemotherapy‐induced diarrhea in patients with DLBCL who have received frontline R‐CHOP chemotherapy, and this has implications for optimizing treatment with such agents. (Cancer Sci 2008; 99: 2496–2501)
Oxysterol binding protein‐related protein‐5 is related to invasion and poor prognosis in pancreatic cancer
Authors: Koga Yoshikatsu , Ishikawa Shinji , Nakamura Tadahiko , Masuda Toshiro , Nagai Yohei , Takamori Hiroshi , Hirota Masahiko , Kanemitsu Keiichiro , Baba Yoshifumi , Baba Hideo 
Published: November 2008

In previous studies, the gene expression profiles of two hamster pancreatic cancer cells with different potentials for invasion and metastasis were analyzed. In the present study, we identified that one of the genes expressed strongly in the highly metastatic cell line is hamster oxysterol binding protein‐related protein (ORP)‐5. The aim of the present study was to clarify the relationship between ORP5 and invasion and poor prognosis of human pancreatic cancer. Invasion assays were carried out in both hamster and human pancreatic cancer cells by suppressing the ORP5 gene with short interfering RNA or inducing its expression by introducing an expression vector. To evaluate the relationship between ORP5 and the characteristics of human pancreatic cancer, 56 pancreatic cancer tissue specimens were analyzed and the ORP5 expression in each pancreatic cancer tissue specimen was analyzed by immunohistochemistry. In both the hamster and human pancreatic cancer cells, suppression of ORP5 significantly reduced the invasion rate of the cells and induction of ORP5 significantly enhanced the invasion rate of the cells. In the clinical sample, the median survival times of the patients with ORP5‐positive (n = 33) and ORP5‐negative (n = 23) cancer were 8.3 and 17.2 months, respectively (P = 0.02). Also, the 1‐year survival rates of patients with ORP5‐positive and ORP5‐negative cancer were 36.4 and 73.9%, respectively (P = 0.005). The ORP5 expression level was related to both invasion and poor prognosis in human pancreatic cancer. These findings suggest that the expression of ORP5 may induce cancer cell invasion, resulting in the poor prognosis of pancreatic cancer. (Cancer Sci 2008; 99: 2387–2394)
B cells play a crucial role as antigen-presenting cells and collaborate with inflammatory cytokines in glucose-6-phosphate isomerase-induced arthritis
Authors: Tanaka-Watanabe Y , Matsumoto I , Iwanami K , Inoue A , Goto D , Ito S , Tsutsumi A , Sumida T 
Published: November 2008

Summary
Anti-glucose-6-phosphate isomerase (GPI) antibodies from K/BxN mice directly induce arthritis; however, the transfer of these antibodies from mice with GPI-induced arthritis does not induce arthritis. CD4+ T cells play an important role in the induction and effector phase in this model; however, the roles of B cells and immunoglobulins (Igs) have not been elucidated. We investigated the roles of B cells and Igs in GPI-induced arthritis by using adoptive transfer system into SCID mice. Transfer of splenocytes of male DBA/1 mice immunized with GPI into SCID mice induced arthritis on day 6 in the latter, in association with the production of anti-GPI antibodies. Co-localization of C3 and IgG on the articular surface was identified in arthritic SCID mice. Inoculation of IgG (or anti-GPI antibodies) and CD19+-depleted splenocytes from arthritic DBA/1 mice induced arthritis in SCID mice, but not CD19+-depleted or CD4+-depleted splenocytes from DBA/1 mice. In vitro analysis of cytokine production by splenocytes from DBA/1 arthritic mice demonstrated production of large amounts of tumour necrosis factor (TNF)-α and interleukin (IL)-6 in an antigen-specific manner (P &lt; 0·01), and production was dominated by CD19+-depleted than CD4+-depleted splenocytes (P &lt; 0·05). Addition of IgG from DBA/1 arthritic mice to the culture enhanced TNF-α but not IL-6 production, and this effect was blocked by anti-Fcγ receptor antibody. In vivo analysis of neutralization with TNF-α protected arthritis completely in SCID mice. Our results highlight the important role of B cells in GPI-induced arthritis as autoantibody producers, and these autoantibodies can trigger joint inflammation in orchestration with inflammatory cytokines, especially TNF-α.
Changes in the long‐term distribution of zooplankton in the Humboldt Current Ecosystem off Peru, 1961–2005, and its relationship to regime shifts and environmental factors
Authors: AYÓN PATRICIA , SWARTZMAN GORDON 
Published: November 2008

AbstractWe used a dataset assembled by the Peruvian Marine Research Institute (IMARPE) from 1961 to 2005, to examine the influence of seasonal, environmental (temperature and salinity), temporal (day–night and year) and location (north‐south and on‐off shelf) variables on the distribution and biovolume of zooplankton. Classification and regression trees (CART) showed that the zooplankton distribution can be divided into four time regimes: prior to 1974.5, 1974.5–1989.5, 1989.5–1997.5 and 1997.5–2005. These periods differ in overall biovolume but, more importantly, in having different spatial distributions of variables. Offshore‐onshore, seasonal, diel and north‐south patterns are all apparent in the temporal changes observed in zooplankton distribution. In the early period zooplankton biovolume was high throughout the Peruvian Humboldt Current Ecosystem, with enhanced biovolume in all seasons but winter. After 1974.5, biovolume was considerably lower. There was an increased difference between the shelf (lower biovolume) and offshore (higher biovolume), with a significant day–night effect offshore, due to diel migration and possibly diel catchability changes. After 1989.5, seasonal effects were more marked and biovolume appeared to be higher in the winter in the northern region. In other seasons, during the daytime, biovolume was highest farther offshore (greater than 65 km offshore of the 200‐m isobath) and, during the night, higher after 1997.5 than before. We relate these changes in distribution and biovolume to changes in large‐scale current patterns in different regimes and to the possible effect of the fishery in removing nutrients from the system.
Environmental and spatial effects on the distribution of blue marlin (Makaira nigricans) as inferred from data for longline fisheries in the Pacific Ocean
Authors: SU NAN‐JAY , SUN CHI‐LU , PUNT ANDRE E. , YEH SU‐ZAN 
Published: November 2008

AbstractBlue marlin is distributed throughout tropical and temperate waters in the Pacific Ocean. However, the preference of this species for particular habitats may impact its vulnerability to being caught. The relationship between spatio‐temporal patterns of blue marlin abundance and environmental factors is examined using generalized additive models fitted to catch and effort data from longline fisheries. The presence of blue marlin, and the catch rate given presence, are modeled separately. Latitude, longitude, and sea‐surface temperature explain the greatest proportion of the deviance. Spatial distributions of relative density of blue marlin, based on combining the probability of presence and relative density given presence, indicate that there is seasonal variation in the distribution of blue marlin, and that the highest densities occur in the tropics. Seasonal patterns in the relative density of blue marlin appear to be related to shifts in SST. The distribution and relative abundance of blue marlin are sufficiently heterogeneous in space and time that the results of analyses of catch and effort data to identify ‘hotspots’ could be used as the basis for time‐area management to reduce the amount of blue marlin bycaught in longline fisheries.
From egg to juvenile in the Bay of Biscay: spatial patterns of anchovy (Engraulis encrasicolus) recruitment in a non‐upwelling region
Authors: IRIGOIEN XABIER , COTANO UNAI , BOYRA GUILLERMO , SANTOS MARIA , ALVAREZ PAULA , OTHEGUY PANTXIKA , ETXEBESTE EGOITZ , URIARTE ANDRES , FERRER LUIS , IBAIBARRIAGA LEIRE 
Published: November 2008

AbstractIn this study the spatial distribution of eggs, larvae and juveniles of European anchovy (Engraulis encrasicolus) was followed in 2004 and 2005 during three consecutive cruises each year in spring–summer to test what the limits of retention are in a non‐upwelling area. Eggs, small larvae and large juveniles were mainly distributed over the shelf, whereas large larvae and small juveniles were found mainly off the shelf. Although overall distributions were similar, the 2 yr differed in that there was more of a coastal distribution of individuals in 2004, whereas in 2005 more individuals were found off the shelf. There were no significant differences in the length–weight relationships for individuals found on and off the shelf or between years. The correspondence in circulation patterns and the lack of difference in the length–weight relationships suggest that a single population is present, larvae drifting off the shelf due to currents and returning as mobile juveniles. Quantile regression analysis of the long‐term recruitment index suggests that transport off the shelf may favour good recruitments. This would suggest that in non‐upwelling regions the retention area resulting in good recruitment may not be restricted to the shelf.
Spatial distribution of ocean habitat of yearling Chinook (Oncorhynchus tshawytscha) and coho (Oncorhynchus kisutch) salmon off Washington and Oregon, USA
Authors: BI HONGSHENG , RUPPEL RACHEL E. , PETERSON WILLIAM T. , CASILLAS EDMUNDO 
Published: November 2008

AbstractWe determined the habitat usage and habitat connectivity of juvenile Chinook (Oncorhynchus tshawytscha) and coho (Oncorhynchus kisutch) salmon in continental shelf waters off Washington and Oregon, based on samples collected every June for 9 yr (1998–2006). Habitat usage and connectivity were evaluated using SeaWiFS satellite‐derived chlorophyll a data and water depth. Logistic regression models were developed for both species, and habitats were first classified using a threshold value estimated from a receiver operating characteristic curve. A Bernoulli random process using catch probabilities from observed data, i.e. the frequency of occurrence of a fish divided by the number of times a station was surveyed, was applied to reclassify stations. Zero‐catch probabilities of yearling Chinook and yearling coho salmon decreased with increases in chlorophyll a concentration, and with decreases in water depth. From 1998 to 2006, ∼ 47% of stations surveyed were classified as unfavorable habitat for yearling Chinook salmon and ∼ 53% for yearling coho salmon. Potentially favorable habitat varied among years and ranged from 9 856 to 15 120 km2 (Chinook) and from 14 800 to 16 736 km2 (coho). For both species, the smallest habitat area occurred in 1998, an El Niño year. Favorable habitats for yearling Chinook salmon were more isolated in 1998 and 2005 than in other years. Both species had larger and more continuous favorable habitat areas along the Washington coast than along the Oregon coast. The favorable habitats were also larger and more continuous nearshore than offshore for both species. Further investigations on large‐scale transport, mesoscale physical features, and prey and predator availability in the study area are necessary to explain the spatial arrangement of juvenile salmon habitats in continental shelf waters.
Effects of substrate availability on the temperature sensitivity of soil organic matter decomposition
Authors: GERSHENSON ALEXANDER , BADER NICHOLAS E. , CHENG WEIXIN 
Published: November 2008

AbstractSoil carbon is a major component in the global carbon cycle. Understanding the relationship between environmental changes and rates of soil respiration is critical for projecting changes in soil carbon fluxes in a changing climate. Although significant attention has been focused on the temperature sensitivity of soil organic matter decomposition, the factors that affect this temperature sensitivity are still debated. In this study, we examined the effects of substrate availability on the temperature sensitivity of soil respiration in several different kinds of soils. We found that increased substrate availability had a significant positive effect on temperature sensitivity, as measured by soil Q10 values, and that this effect was inversely proportional to original substrate availability. This observation can be explained if decomposition follows Michaelis–Menten kinetics. The simple Q10 model was most appropriate in soils with high substrate availability.
Leaf carbon management in slow‐growing plants exposed to elevated CO2
Authors: ARANJUELO IKER , PARDO ANTONI , BIEL CARMEN , SAVÉ ROBERT , AZCÓN‐BIETO JOAQUIM , NOGUÉS SALVADOR 
Published: November 2008

AbstractTwo slow‐growing plant species (Chamaerops humilis, L. and Cycas revoluta Thunb.) were exposed to elevated CO2 conditions over a 20‐month period in order to study the CO2 effect on growth, photosynthetic capacity and leaf carbon (C) management. The ambient isotopic 13C/12C composition (δ13C) of the greenhouse module corresponding to elevated CO2 (800 μmol mol−1 CO2) conditions was changed from δ13C ca. −12.8±0.3‰ to ca. −19.2±0.2‰. Exposure of these plants to elevated CO2 enhanced dry mass (DM) by 82% and 152% in Chamerops and Cycas, respectively, mainly as a consequence of increases in plant level photosynthetic rates. However, analyses of A–Ci curve parameters revealed that elevated CO2 diminished leaf photosynthetic rates of Chamaerops whereas in Cycas, no photosynthetic acclimation was detected. The fact that Chamaerops plants had a lower DM increase, together with a longer leaf C residence time and a diminished capacity to respire recently fixed C, suggests that this species was unable to increase C sink strength. Furthermore, the consequent C source/sink imbalance in Chamaerops might have induced the downregulation of Rubisco. Cycas plants were capable of avoiding photosynthetic downregulation due to a greater ability to increase C sink strength, as was confirmed by DM values, and 12C‐enriched CO2 labeling data. Cycas developed the ability to respire a larger proportion of recently fixed C and to reallocate the recently fixed C away from leaves to other plant tissues. These findings suggest that leaf C management is a key factor in the responsiveness of slow‐growing plants to future CO2 scenarios.