bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dye removal from Waste Water by Java Plum Tree Leaves as an Effective Biosorbent
Authors: Gul Salma , Gul Sadaf , Jamila Nargis , Iqbal Fatima , Gul Hajera , Shahd Tawaf Ali 
Published: February 2024

Abstract
The Neutral Red (NR) dye is an important cationic dye used in various industries, however it pollutes the environment via disposal of industrial waste into water. Therefore, in this research, adsorption process is used, for the removing this dye by using Java plum tree leave as an adsorbent. Various reaction parameters such as contact time, concentration of the dye in solution, dose of the adsorbent, and pH were selected to study the removal of the dye from wastewater. The maximum percent removal 92.5% and Qe value 55.49 mg/g for NR dye removal was achieved at optimized conditions. The result shows that experimental data fitted Freundlich adsorption isotherm with R2 of 0.94. Pseudo First Order kinetics followed the data. The results showed that java plum tree leaves is an inexpensive and environmental friendly adsorbent, which can be used for the removal of cationic NR dye from wastewater.
Development and Assessment of a Mucoadhesive Formulation Incorporating Phenytoin for Wound Healing
Authors: Mehrjardi Soheil Tafazzoli , Jamshidi Hamid Reza , Ramezani Vahid 
Published: February 2024

Abstract
This study aimed to develop flexible wafers for drug delivery to enhance wound healing. Sodium phenytoin, a known therapeutic agent, and chitosan, a biodegradable polymer with wound healing properties, were key components of the formulations. The study investigated the impact of various factors, such as the addition of propylene glycol (PG) to improve flexibility, and the combination of alginate, chitosan, and hydroxypropyl methylcellulose (HPMC) to achieve extended drug release and mucoadhesion. Results showed that increasing the amount of PG in the formulation improved flexibility but reduced mucoadhesion. Alginate and chitosan, when combined, formed polymer filaments, while HPMC and PG demonstrated high mucoadhesion. Drug release tests indicated that formulations with higher HPMC content exhibited slower release rates. Additionally, phenytoin was successfully incorporated into the alginate/chitosan polymer matrix, as confirmed by differential scanning calorimetry (DSC) and electron microscopy. In vivo evaluation using animal models demonstrated the effectiveness of the wafer containing phenytoin sodium in promoting faster wound healing compared to the drug-free wafer and phenytoin ointment. Pathology results revealed increased epidermal cell proliferation, the formation of a comprehensive layer of epidermis, and the appearance of hair follicles and dermis layer in the wafer-treated wounds. Furthermore, the wafer formulation containing 0.01% phenytoin exhibited complete healing by the twelfth day of treatment. The study highlights the potential of sodium phenytoin and the developed wafer formulation in improving wound healing outcomes. The sustained drug release and adhesive mucosa properties of the wafer contribute to its enhanced efficacy. Further research is warranted to gain a comprehensive understanding of the diverse effects of sodium phenytoin on wound healing and optimize the formulation for clinical applications.
Pan-cancer analysis of TMEM45A indicates its potential prognostic value and functional validation in gastric cancer
Authors: Xie Qixin , Guo Tao , Deng Hong , Yu Changjun , Fang Changyi 
Published: February 2024

Abstract
Background
Gastric cancer (GC) is a frequent malignant tumor of the digestive system with the highest incidence and mortality rate among all tumors. This research aimed to investigate the relationships among TMEM45A expression, prognostic values and tumor-infiltrating immune cells (TIICs) in GC patients.
Methods
First, GEO and TCGA database were employed to analyze the expression of TMEM45A in GC patients. Then, we determined the association between TMEM45A expression and survival of GC patients using the Kaplan-Meier Plotter database and TCGA database and verified the accuracy of TMEM45A in predicting prognosis. Next, we analyzed the effect of CTHRC expression on TIICs in GC tissues. A prognostic model was constructed using immunomodulatory genes associated with TMEM45A. The specificity and accuracy of the model were verified.
Results
TMEM45A expression was markedly higher in GC tissue than in normal tissue. GC patients with TMEM45A overexpression had a poor prognosis. The AUC value of 5-year survival on the ROC curve was 0.705, indicating that TMEM45A is a reliable prognostic factor and can be used as a clinicopathological indicator alone to predict patient prognosis. Three high-risk immunomodulatory genes (CXCR4 and TGFB1) and one low-risk immunomodulatory gene (PDCD1) were obtained using both univariate and multivariate COX methods. These three immunomodulatory molecules were used to construct prognostic models.
Conclusion
GC patients with TMEM45A overexpression have a poor prognosis and are associated with immune cell infiltration. Hence, TMEM45A is a fairly reliable independent prognostic marker.
Unveiling Determinants of Household Lighting Preferences in Rural Tanzania: Insights for Sustainable Energy Access
Authors: Kamuzora Aurelia Ngirwa 
Published: February 2024

Abstract
This study delves into the intricate determinants that steer household lighting preferences in rural Tanzania, where reliance on traditional lighting persists despite ongoing electrification efforts. Employing a quantitative methodology, the research scrutinizes factors that either impede or facilitate the adoption of modern and sustainable lighting sources. Grounded in the Random Utility Theory, the investigation utilizes the Multinomial Logit Model to analyze data sourced from the Tanzania Panel Survey of 2020/2021. The findings unearth several significant influencers of lighting choices among households in rural Tanzania. Firstly, household age emerges as a noteworthy factor, displaying a negative correlation with the likelihood of opting for electricity as a lighting source. Additionally, households headed by males exhibit decreased odds of selecting solar and candle lighting alternatives. Moreover, larger households tend to shy away from electricity, preferring alternative lighting options. On the other hand, employment status and higher income levels positively correlate with the adoption of electricity and candle lighting. These findings underscore the multifaceted nature of factors that shape household lighting preferences in rural Tanzanian settings. Consequently, addressing these determinants emerges as pivotal in fostering sustainable and affordable energy access. Policy implications drawn from this research underscore the necessity for targeted interventions that address socioeconomic and demographic dynamics. Such interventions are crucial for enhancing the efficacy of rural electrification initiatives and ultimately improving the quality of life for rural Tanzanian households
Prospective study to evaluate radioactive iodine of 20 mCi vs 10-15 mCi in Graves’ disease
Authors: Kanokwongnuwat Wasit , Penpong Nawarat 
Published: February 2024

Abstract

Objectives
To assess whether increasing radioactive iodine dose can increase treatment efficacy in Graves’ disease.
Methods
A prospective study was conducted, including 106 patients receiving 20 mCi (740 MBq) radioactive iodine (RAI), compared with a retrospective data, including 113 patients receiving 10-15 mCi (370-555 MBq) RAI. Remission and failure rates were evaluated at 6 months post-RAI. Statistical analysis was performed using logistic regression and Kaplan-Meier curves.
Results
Patients receiving 20 mCi RAI demonstrated a significantly higher remission rate compared to the 10-15 mCi group (82.1% vs 66.4%, p = 0.009). Median time to remission was shorter in the 20 mCI group (3 vs 4 months, p = 0.002). Hypothyroidism at 6 months was more prevalent in the 20 mCi group (67% vs 53%, p = 0.03). Larger thyroid size (>60 grams) was associated with treatment failure (p =0.02).
Conclusions
Higher dosage (20 mCi) RAI showed superior efficacy in achieving remission compared to lower dosages (10-15 mCi) in Graves’ disease treatment.
Study of sorption properties of zirconia, alumina and silica in relation to repellents
Authors: Zverev Sergei A. , Vinogradova Yana V. , Selivanova Anna A. , Solovov Roman D. , Sakharov Konstantin A. , Ischenko Anatoliy A. , Andreev Sergei V. 
Published: February 2024

Abstract

In this work, the morphology of zirconia, alumina, and silicas was studied and static sorption of the repellents N,N-diethyl-3-methylbenzamide and ethyl-3-[acetyl(butyl)amino]propionate on these oxides was carried out. ZrO2, Al2O3, SiO2 phenyl were shown to have high sorption activity to the repellents N,N-diethyl-3-methylbenzamide (239 mg/g for SiO2 phenyl) and ethyl-3-[acetyl(butyl)amino]propionate (251 mg/g for ZrO2). Pointedly, it was found that despite having the largest pore volume and high specific surface area (compared to the other studied oxides), SiO2 C2 has a significantly inferior sorption capacity in respect to other oxides, in particular SiO2 phenyl, which can be explained by the presence of the phenyl group in the latter that has chemical affinity for repellent molecules. Obtained isotherms of SiO2 300 also confirm the low sorption activity towards N,N-diethyl-3-methylbenzamide. The sorption equilibrium for both repellents, in most cases, is described by the Langmuir monomolecular adsorption model. The obtained results suggest that the studied zirconia, alumina, and silica can be used as carrier components of repellents.
Long-term Safety and Effectiveness of Lurasidone in Adolescents and Young Adults with Schizophrenia: Pooled Post hoc Analyses of Two 12-month Extension Studies
Authors: Calisti Fabrizio , Tocco Michael , Mao Yongcai , Goldman Robert 
Published: February 2024

Abstract

Objectives
The aim of this analysis was to evaluate the long-term safety and effectiveness of lurasidone in the treatment of schizophrenia in adolescents and young adults (13–25).
Methods
The 2 pooled studies used similar designs and outcome measures. Patients (13–25) with schizophrenia completed an initial double-blind 6-week trial of lurasidone (40 and 80 mg/d) in the adolescent trial and (80 and 160 mg/d) in the young adult trial. In open-label long-term trials, adolescent patients were treated with 20–80 mg/d lurasidone, and adults were treated with 40–160 mg/d lurasidone. Efficacy was evaluated based on the Positive and Negative Syndrome Scale (PANSS) and Clinical Global Impression-Severity Scale (CGI-S).
Results
The safety population consisted of 306 patients (mean age, 16.2 years; 208 patients (68.0%) who completed 12 months of treatment; 8.2% who discontinued treatment by 12 months due to an adverse event). The mean (SD) changes in the PANSS total score from the extension baseline to months 6 and 12 were − 11.8 (13.9) and − 15.3 (15.0), respectively (OC), and the mean (SD) changes in the CGI-S score were − 0.8 (1.0) and − 1.0 (1.1), respectively (OC). The most frequent adverse events were headache (17.6%), anxiety (11.4%), schizophrenia (9.8%), and nausea (9.8%). No clinically meaningful changes were observed in weight, metabolic parameters, or prolactin.
Conclusions
In adolescents and young adults with schizophrenia, treatment with lurasidone was generally well tolerated and effective. Long-term treatment was associated with a continued reduction in symptoms of schizophrenia. Long-term treatment was associated with minimal effects on weight, metabolic parameters, and prolactin.
Clinicaltrials.gov identifiers: D1050234, D1050302
High percentage of bone marrow CD8+ tissue-resident-like T cells predicts inferior survival in patients with acute myeloid leukemia
Authors: Cai Letong , Lai Wenpu , Yao Danlin , Gu Yinfeng , Liang Caofeng , Liu Lian , Lai Jing , Yu Zhi , Zha Xianfeng , Yu Xibao , Wu Xiuli , Chen Shaohua , Luo Oscar Junhong , Li Yangqiu , Wang Chunyan , Qin Pengfei , Huang Xin , Xu Ling 
Published: February 2024

Abstract
Background
Acute myeloid leukemia (AML) is a malignant clonal blood disease and the most common type of acute leukemia in adults. Despite continuous advances in treatments, the long-term prognosis of AML has not improved substantially. Tissue-resident memory T cells (TRMs) infiltrating solid tumors could influence tumor progression and the response to immune therapies; however, the proportion and prognostic significance of TRMs in the bone marrow (BM) of patients with AML are unclear.
Methods
We use flow cytometry to assay the phenotypic of 49 BM samples from patients newly diagnosed with AML (ND-AML). The Kaplan–Meier Plotter database verified the relationship between the expression of CD8+ TRM-like T cell characteristic genes (CD8A, CD69, and TOX) and patient survival. Additionally, to further explored the existence and function of TRM-like CD8+ T cells in the BM by analyzing the single cell proteo-genomic dataset of BM from AML and healthy.
Results
We found that the BM CD8+ effector memory (TEM) cells highly expressed CD69 (CD8+ TRM-like T cells), and their number significantly increased in ND-AML compared with that in HIs. The high CD8+ TRM-like subset is associated with poor overall survival. The Kaplan–Meier Plotter database verified that the survival rate of patients with high expression of CD8+ TRM-like T cell characteristic genes was significantly reduced, especially in the M4 and M5 subtypes. Phenotypic analysis revealed that the BM CD8+ TRM-like subpopulation exhibited exhausted T cell characteristics, but its high expression of CD27 and CD28 and low expression of CD57 suggest its high proliferative potential. The single-cell proteogenomic dataset confirmed the existence of TRM-like CD8+ T cells in the BM of AML and verified the high expression of immune checkpoints and costimulatory molecules.
Conclusions
We found that the accumulation of BM CD8+ TRM-like subset could be considered as an immune related survival prediction marker for patients with AML. Although the mechanisms of BM CD8+ TRM-like subset in driving immune escape in AML remains unknown, we believe that the targeted reversal of the function of this subset through immune checkpoint inhibitors and another immune-related “brake” may benefit the survival of some patients with AML.
Short-term effects of "Yijinjing Wohu Pushi" posture-voice therapy on speech in Parkinson's disease after STN-DBS
Authors: sun xin , Yang Mei , Yan Jin , Wang Linbin , Sun Yuxin , Wang Yong , Yan Shiqing , Li Dianyou , Niu Chuanxin M. 
Published: February 2024

Abstract
Hypokinetic dysarthria in Parkinson's Disease (HD-PD) deteriorates patients' quality of life by impeding communication and social engagement. Existing treatments like levodopa drugs and deep brain stimulation (DBS) can improve motor symptoms but fall short in addressing speech-related impairments; effective speech therapies tend not to mandate the posture, which potentially hinders vocal performance. Here we first proposed a therapeutic protocol that specified a lunging-and-clawing posture (Wohu Pushi method from Yijinjing) during vocalization training. The protocol aimed to ensure that PD patients could accomplish the training even with moderate motor impairments. A total of 20 HD-PD patients with implanted stimulators in subthalamic nuclei (STN-DBS) participated in one session of training, either following our posture-voice therapy (PVT) or the conventional voice therapy (CVT). Results indicated that the maximum phonation time was significantly elongated in PVT(3.85±2.81s) but not in CVT(0.46±2.24s), and the formant-distance was significantly increased in PVT(95.80±112.71Hz) compare to CVT(-47.10±84.28Hz). Our results indicated that by demanding appropriate amount of postural maintenance during vocalization, it might facilitate the recovery of speech-related functions. This study warranted larger-scale clinical trials to understand the impact, limitation, and optimization of posture-voice therapy for HD-PD.
VTANet: A Novel Polyp Segmentation Network Based on the Vision Transformer and Attention Mechanism
Authors: Guo Xinping , Wang Lei , Huang Zizhen , Zhang Yukun , Liu ChunXiang , Li Bin 
Published: February 2024

Abstract
The accurate segmentation of the polyp is very important for the diagnosis and treatment plans of the colorectal cancer. Although the UNet model and the models with the U-shaped structure have achieved great success in polyp image segmentation, they are still limited by the colors, sizes and the shapes of polyps, as well as the low contrast, various noise and blurred edges of the colonoscopy, which are easy to result in a large amount of redundant information, the weak complementarity between different levels of features and inaccurate polyp localization. To deal with the special characteristics of the polyps images and improve the segmentation performance, a new segmentation model named VTANet, which is based the pyramid vision transformer and BAM (Bottleneck Attention Module), is developed. The proposed model consists of four modules: the pyramid vision transformer (PVT) encoder, the Feature Aggregation Module (FAM), the Adaptive Attention Fusion Module (AAFM), and the Aggregation Similarity Module (ASM). The PVT learns a more robust representation model, the FAM enhances the complementarity between features by cascading the encoder features and acquiring richer context and fine-grain features. The AAFM makes the polyp localization more accurate by introducing the BAM attention module to obtain richer details of the polyps. To verify the effectiveness and accuracy, experiments on five popularly used datasets are carefully designed and implemented. The segmentation results of six stat-of-the-art methods fully demonstrate the superiority of the proposed method, in the term of the visual comparison and six quantitative metrics, i.e. the Dice, IoU, Mean Absolute Error, weighted F-measure, S-measure, and E-measure.
Establishment and validation of the prognostic risk model based on the anoikis related genes in esophageal squamous cell carcinoma
Authors: Cao Shasha , Li Ming , Cui Zhiying , Li Yutong , Niu Wei , Zhu Weiwei , Li Junkuo , Duan Lijuan , Lun Shumin , Gao Zhaowei , Zhang Yaowen 
Published: February 2024

Abstract
Background
Esophageal squamous cell carcinoma (ESCC) is a malignant condition in humans. Anoikis related genes (ARGs) are crucial to cancer progression. Therefore, more studies on the relationship between ARGs and ESCC are warranted.
Methods
The study acquired ESCC-related transcriptome data from the cancer genome atlas (TCGA). Differentially expressed ARGs (DE-ARGs) were obtained by performing differential analysis on the training set, and candidates were filtered out by survival analysis of high and low expression groups of DE-ARGs. Prognostic genes were determined by univariate and multivariate Cox and lasso regression based on candidate genes and were analyzed by gene set enrichment analysis (GSEA). A risk model was constructed on the basis of prognostic gene expressions. To find out how these genes contribute to ESCC development, immune infiltration study was done. Based on prognostic genes, the miRNA-mRNA-TF regulation network was constructed. IC50 test was adopted to assess the clinical response of chemotherapy drug. Single-cell analysis was performed on the GSE145370 dataset. Moreover, with the use of quantitative reverse transcription (qRT)-PCR, we verified prognostic gene expressions.
Results
53 DE-ARGs (46 upregulated; 7 downregulated) were screened by differential analysis. Survival analysis yielded four candidate genes consisting of PBK, LAMC2, TNFSF10 and KL. The two prognostic genes, TNFSF10 and PBK, were determined by univariate and multivariate Cox and lasso regression. In terms of hallmark, TNFSF10 was involved in 32 pathways, and PBK was partake in 34 pathways. In immunomic signatures, 4558 enrichment entries were associated with TNFSF10, like genes downregulated CD8 T cells, PBK enriched to 4262 pathways, such as genes downregulated in B cells. Immuno-infiltration analysis revealed positive associations of PBK with Macrophages M0 cells, and TNFSF10 with Macrophages M1 cells. miRNA-mRNA-TF network was generated with prognostic genes, which contained hsa-miR-562-TNFSF10-FOXO3, hsa-miR-216b-5p-PBK-ATM regulatory relationship pairs, etc. The result of chemotherapy drug susceptibility analysis showed that IC50 values of predicted drugs, in the case of Tozasertib 1096 and WIKI4 1940, were significantly variant between risk groups. Single-cell analysis revealed that TNFSF10 and PBK levels were higher in epithelial cells than in other cells. The prognostic genes expression results by qRT-PCR were compatible with the dataset analysis.
Conclusion
The study explored the biomarkers related to anoikis based on bioinformatics technology and established a prognosis model of ESCC. It provided a reference for the research of ARGs in ESCC.
Oral Enzyme-Responsive Nanoprobes for Targeted Theranostics of Inflammatory Bowel Disease
Authors: Cao Lin , Duan Dengyi , Guo Yunfei , Li Xinwen , Cao Qi , Li Jianmin , Liu Kangkang , Li Yiming , Zhang Wenyi , Liu Shuang , Zhang Xuening , Peng Jing , Zhao Yang 
Published: February 2024

Abstract

Background
Inflammatory bowel disease (IBD) is a progressive and debilitating inflammatory disease of the gastrointestinal tract (GIT). Despite recent advances, precise treatment and noninvasive monitoring remain challenging.
Methods
Herein, we developed orally-administered, colitis-targeting and hyaluronic acid (HA)-modified, core-shell curcumin (Cur)- and cerium oxide (CeO2)-loaded nanoprobes (Cur@PC-HA/CeO2 NPs) for computed tomography (CT) imaging-guided treatment and monitoring of IBD in living mice.
Results
Following oral administration, high-molecular-weight HA maintains integrity with little absorption in the upper GIT, and then actively accumulates at local colitis sites owing to its colitis-targeting ability, leading to specific CT enhancement lasting for 24 h. The retained NPs are further degraded by hyaluronidase in the colon to release Cur and CeO2, thereby exerting anti-inflammatory and antioxidant effects. Combined with the ability of NPs to regulate intestinal flora, the oral NPs result in substantial relief in symptoms. Following multiple treatments, the gradually decreasing range of the colon with high CT attenuation correlates with the change in the clinical biomarkers, indicating the feasibility of treatment response and remission.
Conclusion
This study provides a proof-of-concept for the design of a novel theranostic integration strategy for concomitant IBD treatment and the real-time monitoring of treatment responses.
RETRACTED: Evaluation of the pest status and population monitoring for the new invasive pest, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), in various crop systems of Georgia (Sakartvelo)
Authors: Japoshvili George , Murvanidze Maka , Inasaridze Nino , Meskhi Nikoloz , Lipartia Zurab , Namicheishvili Luiza 
Published: February 2024

Abstract

The authors have requested that this preprint be removed from Research Square.