bluuebunny/crossref_metadata_2025_split huggingface dataset:
Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes
Authors: Wong Siu-Shing , Monteiro Joao M. , Chang Chia-Chun , Peng Min , Mohamad Nada , Steinacker Thomas L. , Saurya Saroj , Wainman Alan , Raff Jordan W. 
Published: April 2024

AbstractMitotic centrosomes form when centrioles recruit large amounts of pericentriolar material (PCM) around themselves. The PCM comprises hundreds of proteins, yet it can assemble and disassemble within minutes, leading to much debate about its physical nature. Here we show thatDrosophilaSpd-2 fluxes out from centrioles, recruiting Polo and Aurora A to catalyse the assembly of a solid-like Cnn-scaffold and a more liquid-like TACC-scaffold, respectively. Both scaffolds can assemble and recruit PCM proteins independently, but both are required for proper centrosome assembly, with the Cnn-scaffold providing mechanical strength, and the TACC-scaffold locally concentrating centriole and centrosome proteins. Recruiting Spd-2, but not Cnn or TACC, to the surface of synthetic beads injected into early embryos reconstitutes several aspects of mitotic centrosome assembly on the bead surface, and this depends on the ability of Spd-2 to recruit Polo and AurA. Thus, Spd-2 molecules flux out from the centriole recruiting Polo and AurA to promote the assembly of two scaffolds with distinct properties to support mitotic centrosome assembly in flies.
Modelling dynamic host-pathway interactions at the genome scale
Authors: Merzbacher Charlotte , Aodha Oisin Mac , Oyarzún Diego A. 
Published: April 2024

AbstractPathway engineering offers a promising avenue for sustainable chemical production. The design of efficient production systems requires understanding complex host-pathway interactions that shape the metabolic phenotype. While genome-scale metabolic models are widespread tools for studying static host-pathway interactions, it remains a challenge to predict dynamic effects such as metabolite accumulation or enzyme overexpression during the course of fermentation. Here, we propose a novel strategy to integrate kinetic pathway models with genome-scale metabolic models of the production host. Our method enables the simulation of the local nonlinear dynamics of pathway enzymes and metabolites, informed by the global metabolic state of the host as predicted by Flux Balance Analysis (FBA). To reduce computational costs, we make extensive use of surrogate machine learning models to replace FBA calculations, achieving simulation speed-ups of at least two orders of magnitude. Through case studies on two production pathways inEscherichia coli, we demonstrate the consistency of our simulations and the ability to predict metabolite dynamics under genetic perturbations and various carbon sources. We showcase the utility of our method for screening dynamic control circuits through large-scale parameter sampling and mixed-integer optimization. Our work links together genome-scale and kinetic models into a comprehensive framework for computational strain design.
Nitrosation of CD36 regulates endothelial function and serum lipids
Authors: Luse Melissa A. , Schug Wyatt J. , Dunaway Luke S. , Nyshadham Shruthi , Loeb Skylar A. , Carvalho Alicia , Tessema Rachel , Pavelic Caitlin , Keller T.C. Stevenson , Shu Xiaohong , Ruddiman Claire A. , Kosmach Anna , Sveeggen Timothy M. , Mitchell Ray , Bagher Pooneh , Minshall Richard D. , Leitnger Norbert , Columbus Linda , Levental Kandice R. , Levental Ilya , Cortese-Krott Miriam , Isakson Brant E. 
Published: April 2024

AbstractDuring obesity, endothelial cells (ECs) become lipid laden leading to endothelial dysfunction. We demonstrate endothelium downregulates caveolin-1 (Cav1) in mouse and human in response to obesity. Using an EC-specific Cav1 knockout mouse, we find mice are hyperlipidemic regardless of diet, but retain endothelial cell function. Whereas initially this was thought to be due to Cav1 mediate endocytosis, we find instead the mice have significantly increased nitric oxide (NO) in response to the lack of Cav1. The presence or absence of NO toggled inversely EC lipid content and plasma lipid in mice. We found the fatty acid translocase CD36 was directly nitrosated by endogenous NO at the same cysteines that are palmitoylated on CD36. The nitrosation of CD36 prevented it’s trafficking to the plasma membrane and decreased lipid uptake. The physiological effect of this mechanism was a reliance on NO for endothelial function. This work suggests that CD36 nitrosation occurs as a protective mechanism to prevent EC lipotoxicity and preserve function.TeaserNitric oxide regulates serum lipids and endothelial cell lipid content through nitrosation of CD36.
Reproducing Human Subjective Evaluation in the Microscopic Agglutination Test with Deep Learning
Authors: Nakano Risa , Oyamada Yuji , Ozuru Ryo , Miyahara Satoshi , Yoshimura Michinobu , Hiromatsu Kenji 
Published: April 2024

ABSTRACTThe Microscopic Agglutination Test (MAT) is widely recognized as the gold standard for diagnosing zoonosis leptospirosis. However, a significant limitation of MAT is the inconsistency in test results, as it relies on the examiners’ subjective perceptions to estimate agglutination rates. To address this issue, we propose a deep neural network to replicate the subjective evaluation process of agglutination rate estimation in MAT. By employing a pre-trained DenseNet121, we can efficiently optimize the network parameters during the training phase. We validated the trained network using our original dataset. Experimental results demonstrate that the proposed network provides accurate agglutination rate estimates. Furthermore, we utilize a standard visualization technique to gain insights into the decision-making process of the deep learning methods. The findings reveal that the proposed network extracts image features indicative of leptospire abundance. Overall, these results suggest that deep learning is effective for estimating agglutination rates and that enhancing interpretability aids medical experts in understanding the functionality of deep learning.
Single-cell proteomics differentiates Arabidopsis root cell types
Authors: Montes Christian , Zhang Jingyuan , Nolan Trevor M. , Walley Justin W. 
Published: April 2024

AbstractRecently, major advances have enabled the exploration of cellular heterogeneity using single-cell proteomics. Here we examine the feasibility of single-cell proteomics on plant samples. We focus onArabidopsis thaliana, examining isolated single cells from the cortex and endodermis, which are two adjacent root cell-types derived from a common stem cell. From 756 cells we identify 3,763 proteins and 1,118 proteins/cell. Ultimately, we focus on 3,217 proteins quantified following stringent filtering. Of these, we identified 596 proteins whose expression is enriched in either the cortex or endodermis and are able to differentiate these closely related plant cell-types. Collectivity, our findings underscore the promise of single-cell proteomics to explore the heterogeneity of expression between individual plant cells.
Harnessing RNA-based DNA repair pathways for targeted gene editing
Authors: Huynh Nhan , Kwon Sin , McMurrough Thomas A. , Loedige Kurt W. , Tavassoli Marjan , Shao Weijuan , Qin Heyuan , Luu Khanh , Dharni Balpreet , Haydaychuk Olha , Stead Brent E. , Edgell David R. 
Published: April 2024

ABSTRACTRecent studies have revealed a role for RNA in the repair of DNA double-strand breaks. Here, we show that the asymmetric DNA overhangs generated by the small TevSaCas9 dual nuclease informs a simple and robust editing strategy in human cells whereby Polθ and Rad52 are recruited to repair the double-strand break. The 2-nt, 3’ DNA overhang generated by the I-TevI nuclease domain of TevSaCas9 hybridizes with the 3’ end of a co-localized repair template guide RNA to specifically license repair. Substitutions that destabilize the repair duplex reduce editing efficiency. Targeted RNA-templated repair (rep-editing) harnesses cellular RNA-based DNA repair pathways to introduce precise nucleotide edits, deletions and insertions in human cells with high efficiency and fidelity independent of co-delivered repair functions. The small size of TevSaCas9 and RNA repair template offers delivery advantages over size-constrained or multi-component editing systems.
Bank performance and financial stability during the COVID-19 pandemic: lessons from the MENA region
Authors: Mateev Miroslav , Sahyouni Ahmad , Moudud-Ul-Huq Syed , Nair Kiran 
Published: April 2024

PurposeThis study investigates the role of market concentration and efficiency in banking system stability during the COVID-19 pandemic. We empirically test the hypothesis that market concentration and efficiency are significant determinants of bank performance and stability during the time of crises, using a sample of 575 banks in 20 countries in the Middle East and North Africa (MENA).Design/methodology/approachThe main sources of bank data are the BankScope and BankFocus (Bureau van Dijk) databases, World Bank development indicators, and official websites of banks in MENA countries. This study combined descriptive and analytical approaches. We utilize a panel dataset and adopt panel data econometric techniques such as fixed/random effects and the Generalized Method of Moments (GMM) estimator.FindingsThe results reveal that market concentration negatively affects bank profitability, whereas improved efficiency further enhances bank performance and contributes to the banking sector’s overall stability. Furthermore, our analysis indicates that during the COVID-19 pandemic, bank stability strongly depended on the level of market concentration, but not on bank efficiency. However, more efficient banks are more profitable and stable if the banking institutions are Islamic. Similarly, Islamic banks with the same level of efficiency demonstrated better overall financial performance during the pandemic than their conventional peers did.Research limitations/implicationsThe main limitation is related to the period of COVID-19 pandemic that was covered in this paper (2020–2021). Therefore, further investigation of the COVID-19 effects on bank profitability and risk will require an extended period of the pandemic crisis, including 2022.Practical implicationsThis study provides information that will enable bank managers and policymakers in MENA countries to assess the growing impact of market concentration and efficiency on the banking sector stability. It also helps them in formulating suitable strategies to mitigate the adverse consequences of the COVID-19 pandemic. Our recommendations are useful guides for policymakers and regulators in countries where Islamic and conventional banking systems co-exist and compete, based on different business models and risk management practices.Originality/valueThe authors contribute to the banking stability literature by investigating the role of market concentration and efficiency as the main determinants of bank performance and stability during the COVID-19 pandemic. This study is the first to analyze banking sector stability in the MENA region, using both individual and risk-adjusted aggregated performance measures.
Maintaining life satisfaction when faced with multiple crisis episodes: the role of entrepreneurs’ goal orientation
Authors: Knockaert Mirjam , DeTienne Dawn , Coppens Karlien , Lambrecht Johan 
Published: April 2024

PurposeThe aim is to understand how goal orientation may help entrepreneurs maintain life satisfaction when faced with multiple crisis episodes. To do so, the authors study the early impact of COVID-19 on the life satisfaction of entrepreneurs who were previously faced with venture distress.Design/methodology/approachData on 164 Belgian entrepreneurs, who were faced with previous venture distress, are used. The analysis is quantitative, and uses survey data, in combination with databases. The survey was administered in the beginning of the COVID-19 pandemic (June 2020).FindingsEntrepreneurs high in learning goal orientation are less likely, and those high in performance-avoidance orientation are more likely to experience a decline in life satisfaction. Additionally, the time span between the two crises moderates the relationship between learning goal orientation and life satisfaction. Particularly, entrepreneurs high in learning goal orientation are less likely to experience a decline in life satisfaction if more time has passed between venture distress and the COVID-19 crisis.Practical implicationsOften, entrepreneurs are faced with multiple episodes of distress. The authors study entrepreneurs who have been in venture distress before and are then confronted with the COVID-19 crisis. The study shows that goal orientation, which is a type of self-regulation, plays an important role in how entrepreneurs’ life satisfaction is affected. Also, the authors find that the time between distress events matters. These are important insights for practitioners, including entrepreneurs and support providers.Originality/valueThe study adds to the emerging discussion on the impact of crises on entrepreneurs. It studies life satisfaction (a measure of well-being) and complements studies that examine the impact of crises, on entrepreneurs’ actions and firm performance.
Peer mentors' experiences in an online STEM peer mentoring program: “beacons of light”
Authors: Wendt Jillian L. , Jones Vivian O. 
Published: April 2024

PurposeRacially and ethnically minoritized (REM) women continue to be underrepresented in science, technology, engineering and mathematics (STEM) programs and careers. Peer mentoring is one strategy that can support their participation. This study explores the experiences of Black women peer mentors in an online peer mentoring program at two historically Black institutions.Design/methodology/approachA qualitative case study approach was utilized to explore the impact of an online peer mentoring program on peer mentors' STEM self-efficacy, sense of community, STEM identity and intent to persist in STEM.FindingsAnalysis identified five themes relating to peer mentors' experiences in the program: (1) an “I can do this” approach: confidence and self-efficacy; (2) utility of like others; (3) “beacons of light”: intersecting and malleable identities; (4) skills development and (5) motivation and reciprocity. Further, challenges of the online relationship were shared.Originality/valueThe study contributes to the body of knowledge by demonstrating the utility of an online peer mentoring model among women mentors enrolled in STEM programs at two historically Black institutions. The findings support those who are historically marginalized in participating in and remaining in STEM.
Fit-intrapreneurship in the emerging economy of health and fitness industry
Authors: Lara-Bocanegra Alejandro , Pedragosa Vera , García-Fernández Jerónimo , Bohórquez María Rocío 
Published: April 2024

Purpose
This study aims to analyze the precursors of high and low intrapreneurial intentions among fitness center employees, considering various variables (gender, age, organization size and job satisfaction).

Design/methodology/approach
The study involved 166 fitness center employees of the Portuguese fitness center. The study used a two-part questionnaire to gather sociodemographic data and assess variables related to intrapreneurial intentions and job satisfaction among fitness employees. The first part collected basic demographic information, while the second used validated scales to measure intrapreneurial intentions (innovation and risk-taking) and job satisfaction (intrinsic and extrinsic).

Findings
This study underscores intrapreneurship as key for the evolving global fitness sector, highlighting job satisfaction as critical for fostering intrapreneurial intentions. Age, organizational size and gender diversity are also significant, suggesting that fostering a diverse and satisfied workforce under transformational leadership can enhance fitness organizations’ adaptability and growth.

Social implications
This research supports the growth of the fitness sector by demonstrating how intrapreneurship, propelled by job satisfaction, can resolve challenges, benefiting fitness centers regardless of size, age or gender diversity.

Originality/value
The study highlights the vital role of intrapreneurs in the fitness industry, advocating a nongender-biased approach to intrapreneurship and identifying job satisfaction as key to fostering intrapreneurial intentions, beneficial for all fitness centers.
Bloodmobile location selection for resilient blood supply chain: a novel spherical fuzzy AHP-integrated spherical fuzzy COPRAS methodology
Authors: Imamoglu Gul , Ayyildiz Ertugrul , Aydin Nezir , Topcu Y. Ilker 
Published: April 2024

PurposeBlood availability is critical for saving lives in various healthcare services. Ensuring blood availability can only be achieved through efficient management of the blood supply chain (BSC). A key component of the BSC is bloodmobiles, which are responsible for a significant portion of blood donation collections. The most crucial factor affecting the efficacy of bloodmobiles is their location selection. Therefore, detailed decision analyses are essential for the location selection of bloodmobiles. This study proposes a comprehensive approach to bloodmobile location selection for resilient BSCs.Design/methodology/approachThis study provides a novel integration of the spherical fuzzy analytical hierarchy process (SF-AHP) and spherical fuzzy complex proportional assessment (SF-COPRAS) methodologies. In this framework, the criteria are weighted using SF-AHP. The alternatives are then evaluated using SF-COPRAS, employing criteria weights obtained from SF-AHP without defuzzification.FindingsThe results show that supply conditions and resilience are the most important criteria for a bloodmobile location selection. Additionally, the validation analyses confirm the stability of the solution.Practical implicationsThis study presents several managerial implications that can aid mid-level managers in the BSC during the decision-making process for bloodmobile location selection. The critical factors revealed, along with their importance in choosing bloodmobile locations, serve as a comprehensive guide. Additionally, the framework proposed in this study offers decision-makers (DMs) an effective method for ranking potential bloodmobile locations.Originality/valueThis study presents the first application of multi-criteria decision-making (MCDM) for bloodmobile location selection. In this manner, several aspects of bloodmobile location selection are considered for the first time in the existing literature. Furthermore, from the methodological aspect, this study provides a novel SF-AHP-integrated SF-COPRAS methodology.
Economic freedom and foreign direct investment in Brazil: an empirical analysis of determinants and policy implications
Authors: Upadhyaya Kamal , Barreto de Góes Bruno 
Published: April 2024

Purpose
This paper aims to study the impact of economic freedom and some key macroeconomic variables on the foreign direct investment (FDI) inflow in Brazil.

Design/methodology/approach
An econometric model is developed that includes FDI inflow as the dependent variable and macroeconomic variables such as the output, current account balance, the real exchange rate, openness and economic freedom as explanatory variables. Annual time series data from 1995 to 2022 is used. Before carrying out the estimation, the time series properties of the data are diagnosed using unit root tests and cointegration tests. Since the data series were found to be stationary in the first difference form and the variables in the model were cointegrated, an error correction model is developed and estimated.

Findings
The findings demonstrate that the size of the market (gross domestic product), current account balance and the economic freedom index significantly influence FDI inflow to Brazil. Although the signs of openness and the real exchange rate align with theoretical expectations, they do not attain statistical significance.

Originality/value
To the best of the authors’ knowledge, this is the first formal study on the impact of economic freedom on the FDI inflow in Brazil. The finding of this study adds value to the understanding of FDI dynamics in Brazil, highlighting the critical role of economic freedom and market size in attracting foreign investment.
Effect of intellectual capital on organizational performance in the Indonesian SOEs and subsidiaries: roles of open innovation and organizational inertia
Authors: Tjahjadi Bambang , Soewarno Noorlailie , Sutarsa Annisa Ayu Putri , Jermias Johnny 
Published: April 2024

PurposeThis study aims to investigate the direct effect of intellectual capital on the organizational performance of Indonesian state-owned enterprises (SOEs) and their subsidiaries. Furthermore, it also examines whether the relationship is mediated by open innovation and moderated by organizational inertia.Design/methodology/approachThis study is designed as quantitative research. A survey method is employed to collect data by distributing questionnaires to the upper-level managers of the SOEs and their subsidiaries. A total of 293 questionnaires were distributed to the respondents, and 97 responses were obtained for further analysis. The partial least square structural equation modeling (PLS-SEM) is used to test the hypotheses. A mediation-moderation research framework is employed.FindingsThe results show that intellectual capital has a positive effect on organizational performance. Further results also demonstrate that open innovation mediates the intellectual capital–organizational performance relationship and organizational inertia moderates the intellectual capital–organizational performance relationship. Theoretically, the findings contribute to the resource-based view (RBV) and knowledge-based view (KBV) by providing empirical evidence of the importance of distinctive internal resources in achieving superior organizational performance. Practically, the findings provide strategic information for managers that they should properly manage intellectual capital, open innovation and organizational inertia because of their effects on organizational performance.Originality/valueFirst, this study addresses the previous research gaps by confirming that intellectual capital has a positive effect on organizational performance in the research setting of an emerging market. Second, by using a mediation research framework, this study shows that open innovation mediates the relationship between intellectual capital and organizational performance. Third, by using a moderating research framework, this study also reveals that organizational inertia weakens the relationship between intellectual capital and organizational performance. Those associations are rarely researched.