bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Bioinformatics Analysis Reveals the Role of Shared Cuproptosis‐ and Ferroptosis‐Related DEG DLD in Abdominal Aortic Aneurysm
Authors: Hu Xingwei , Hu Lu , Si Xiaoyun , Feng Qian , Ma Yi , Liu Zhijiang , He Xiang , Shi Bei 
Published: February 2025

ABSTRACTFerroptosis plays a crucial role in the progression of abdominal aortic aneurysm (AAA). Cuproptosis, as a new mode of death, has some similarities with ferroptosis. The primary objective of this study was to develop the role of shared cuproptosis‐related differentially expressed genes (CRDEGs) and ferroptosis‐related differentially expressed genes (FRDEGs) in AAA. RNA sequencing and bioinformatic analyses of human AAA tissue were used to identify dihydrolipoamide dehydrogenase (DLD), which is involved in cuproptosis and ferroptosis. qRT‐PCR and IHC assays further confirmed that the DLD level was substantially higher in the AAA group than in the control group. Finally, experimental verification was conducted to identify that DLD could promote the necrosis, apoptosis and mitophagy of SMCs. In summary, our research identified DLD, linked to cuproptosis and ferroptosis, as differentially expressed in AAA across human and murine samples. DLD's role in regulating SMC necrosis, apoptosis and mitophagy positions it as a potential AAA biomarker and therapeutic target, warranting further investigation for clinical applications.
Grading Right‐to‐Left Shunt With Transforaminal Doppler: A Valid Approach in Patients With Cryptogenic Stroke
Authors: Martínez García Beatriz , Chico García Juan Luis , Pérez Gil Daniel , Garay Albízuri Patricia , Llanes Ferrer Ana , García Alcántara Gabriel , Cabañas Engenios Gloria , Matute Lozano María Consuelo , De Felipe Mimbrera Alicia , Vera Lechuga Rocío , Cruz Culebras Antonio , Carrión Sánchez Irene , Fernández‐Golfín Covadonga , Masjuan Jaime , García Madrona Sebastián 
Published: February 2025

ABSTRACTBackground and PurposeRight‐to‐left shunt (RLS), usually related to a patent foramen ovale (PFO), is an important cause of cryptogenic stroke (CS) in young patients. Transcranial Doppler (TCD) with an agitated saline bubble study is a highly sensitive modality for RLS diagnosis using a transtemporal approach (TTD). However, a minority of patients have insufficient temporal bone windows. We aimed to evaluate the accuracy of transforaminal TCD (TFD) for RLS diagnosis.MethodsWe included patients with CS or transient ischemic attack who were tested in the standard protocol for RLS between March 2022 and February 2023. We evaluated the concordance of RLS grades between TFD and transesophageal echocardiogram (TEE) using the weighted kappa index. We also compared TTD and TFD approaches.ResultsForty patients were included (66.7% men; median age 49 years). Medium or large RLS were found in 28 patients (70%) with TTD and in 27 patients with TFD (67.5%). Through TEE, 19 (82.6%) medium or large PFO were confirmed, and a high agreement rate of 0.684 (p = 0.0003) was observed for grading RLS. Moreover, the agreement rate of RLS grade between TTD and TFD was 0.73 (p < 0.0001).ConclusionTFD is a valid approach for RLS diagnosis, with substantial concordance with TEE in grading RLS. Our study found a good grade of agreement between TFD and TEE. Therefore, the value of TCD goes beyond quantifying RLS and could assist in identifying the patients with the largest RLS, who would gain the greatest benefit from PFO closure.
Analysis of the Northeast Constitutional Financing Fund (FNE) on Municipal Economic Indicators in the Period 2010–2020
Authors: Monte Kerlen Andrade Do , Irffi Guilherme , de Sousa Bastos Felipe , Carneiro Diego Rafael Fonseca 
Published: February 2025

ABSTRACTThis paper aims to evaluate the effects of the Constitutional Fund for Financing the Northeast (FNE) on the regional economy during the period from 2010 to 2020. To achieve this objective, we used the Generalized Propensity Score (GPS) econometric methodology, applying the analysis of dose–response functions proposed by Imbens (2000) and Hirano and Imbens (2004). We found evidence of positive and increasing impacts on the economic indicators of the municipalities benefiting from the FNE. Notably, we observed significant effects on Average Income, GDP per capita and Wage Mass when the resources were directed to women and legal entities. The results also suggest that there is some heterogeneity in the effect of the FNE according to the magnitude of the volume of credit contracted, with the exception of Average Income, which does not show an effect when the resources were contracted by women. In the case of total transfers, positive impacts on average income, GDP per capita and wage mass were observed only in the last levels of treatment. In summary, we can infer that FNE resources have a positive impact on the economic indicators of municipalities with higher contracted values.
Geography and Environment Shape Spatial Genetic Variation and Predict Climate Maladaptation Across Isolated and Disjunct Populations of Pinus muricata
Authors: Galland Lanie M. , Faske Trevor M. , Osuna‐Mascaró Carolina , Bisbing Sarah M. , Parchman Thomas L. 
Published: February 2025

ABSTRACTAssessing the evolutionary potential of rare species with limited migration amidst ongoing climate change requires an understanding of patterns of genetic variation and local adaptation. In contrast to the large distributions and population sizes of most pines, Pinus muricata (bishop pine) occurs in a few isolated populations along coastal western North America and is listed as threatened by the IUCN. To quantify how current genetic variation is influenced by distribution and environment, we generated reduced representation DNA sequencing data for most extant populations of P. muricata (12 locations, 7828 loci). We assessed geographic variation in differentiation and diversity and used genetic‐environment association (GEA) analyses to characterise the contribution of environmental variables to local adaptation and genetic structure. Based on these inferences, we quantified genomic offset as a relative estimate of potential maladaptation under mild (SSP1‐2.6) and severe (SSP5‐8.5) climate change scenarios across 2041–2060 and 2081–2100. Despite occurring in small, isolated populations, genetic diversity was not low in P. muricata. Population differentiation was, however, defined across a hierarchy of spatial scales, with stands generally forming genetically identifiable groups across latitude and environments. GEA analyses implicated temperature‐ and soil‐related variables as most strongly contributing to local adaptation. Estimates of maladaptation to future climate varied non‐linearly with latitude, increased with severity of projections and over time, and were predicted by increases in annual temperature. Our results suggest that isolation and local adaptation have shaped genetic variation among disjunct populations and that these factors may shape maladaptation risk under projected climate change.
The SnRK1‐JMJ15‐CRF6 module integrates energy and mitochondrial signaling to balance growth and the oxidative stress response in Arabidopsis
Authors: Zhao Yanming , Wang Xinying , Lei Qianyan , Zhang Xiaoyan , Wang Yubei , Ji Huijia , Ma Chongyang , Wang Pengcheng , Song Chun‐Peng , Zhu Xiaohong 
Published: February 2025

Summary
Mitochondria support plant growth and adaptation via energy production and signaling pathways. However, how mitochondria control the transition between growth and stress response is largely unknown in plants.
Using molecular approaches, we identified the histone H3K4me3 demethylase JMJ15 and the transcription factor CRF6 as targets of SnRK1 in Arabidopsis. By analyzing antimycin A (AA)–triggered mitochondrial stress, we explored how SnRK1, JMJ15, and CRF6 form a regulatory module that gauges mitochondrial status to balance growth and the oxidative stress response.
SnRK1a1, a catalytic α‐subunit of SnRK1, phosphorylates and destabilizes JMJ15 to inhibit its H3K4me3 demethylase activity. While SnRK1a1 does not phosphorylate CRF6, it promotes its degradation via the proteasome pathway. CRF6 interacts with JMJ15 and prevents its SnRK1a1 phosphorylation‐dependent degradation, forming an antagonistic feedback loop. SnRK1a1, JMJ15, and CRF6 are required for transcriptional reprogramming in response to AA stress. The transcriptome profiles of jmj15 and crf6 mutants were highly correlated with those of plants overexpressing SnRK1a1 under both normal and AA stress conditions. Genetic analysis revealed that CRF6 acts downstream of SnRK1 and JMJ15.
Our findings identify the SnRK1‐JMJ15‐CRF6 module that integrates energy and mitochondrial signaling for the growth–defense trade‐off, highlighting an epigenetic mechanism underlying mitonuclear communication.
A peritidal Burgess‐Shale‐type fauna from the middle Cambrian of western Canada
Authors: Mussini Giovanni , Veenma Yorick P. , Butterfield Nicholas J. 
Published: February 2025

AbstractBurgess‐Shale‐type (BST) faunas have proven critical for mapping the Cambrian assembly of animal‐dominated ecosystems, but have so far only been reported from fully subaqueous deposits. Here we integrate evidence from ichnofossils, sedimentary features, and small carbonaceous fossils (SCFs) from the middle Cambrian (Late Guzhangian, Series 3) Pika Formation of western Jasper National Park, Alberta (Canada) to document a unique BST fauna, occupying a peritidal habitat near the outer margin of a large epicratonic sea. Finely laminated shales with mudcracks and dumbbell‐shaped Arthraria‐type burrows denote a periodically emergent, dysoxic mudflat setting. This same facies yields SCF priapulids, annelids and wiwaxiids typical of deeper‐marine sediments. Recovery of Cirratuliformia‐like annelid chaetae further identifies the likely source of Arthraria burrows and associated faecal pellets. These findings show that Cambrian marine metazoans, including probable members of crown‐group orders, ranged beyond permanently subaqueous deposits. The expanded palaeoenvironmental range of the BST taxa from the Pika biota denotes remarkably broad ecological tolerances, suggesting the existence of a guild of Cambrian metazoan generalists able to colonize at least transiently subaerial settings. Their occupation of offshore peritidal ecologies may have preluded to more extensive metazoan colonization of high‐energy, siliciclastic marginal marine environments.
Melanin and Neurotransmitter Signalling Genes Are Differentially Co‐Expressed in Growing Feathers of White and Rufous Barn Owls
Authors: Ducrest Anne‐Lyse , San‐Jose Luis M. , Neuenschwander Samuel , Schmid‐Siegert Emanuel , Simon Céline , Pagni Marco , Iseli Christian , Richter Hannes , Guex Nicolas , Cumer Tristan , Beaudoing Emmanuel , Dupasquier Mélanie , Charruau Pauline , Ducouret Pauline , Xenarios Ioannis , Goudet Jérôme , Roulin Alexandre 
Published: February 2025

ABSTRACTRegulation of melanin‐based pigmentation is complex, involving multiple genes. Because different genes can contribute to the same pigmentation phenotype, the genes identified in model organisms may not necessarily apply to wild species. In the barn owl (Tyto alba), ventral plumage colour ranges from white to rufous, with genetic variation in the melanocortin 1 receptor gene (MC1R) accounting for at least a third of this variation. In the present study, we used transcriptomic data to compare the gene expression profiles of growing feathers from nestlings with different MC1R genotypes. We identified 21 differentially expressed genes, nine of which are involved in melanogenesis, while seven are related to neurotransmitter function or synaptic activity. With the exception of CALB1, all of the differentially expressed genes were upregulated in rufous owls compared to white barn owls. To the best of our knowledge, this study is the first to link melanin production with neurotransmitter‐related genes, and we discuss possible evolutionary explanations for this connection.
Taxing Language: Do Interpreting Fees Affect Immigrant Healthcare Usage? Evidence From a Regression Discontinuity Design
Authors: Halling Aske 
Published: February 2025

ABSTRACTResearch has shown that administrative burdens significantly influence benefit uptake across various welfare programs in the U.S. and beyond. However, much of the existing research has focused primarily on program take‐up, leaving a gap in our understanding of how burdens affect the ongoing use of welfare benefits. To address this gap, we utilize a regression discontinuity design to analyze how the introduction of substantial fees on interpreting services affects usage of healthcare services among Danish immigrants. Our data comes from highly reliable, detailed registries that track the weekly use of general practitioners by Danish immigrants. We find no effects of the fees on healthcare utilization and no evidence that the fees disproportionately affect disadvantaged groups. Additional survey‐based analyses suggest that doctors may use their discretion to mitigate the fee's impact on immigrants, highlighting how frontline workers can potentially reduce the effects of burdensome policies.
Views from the East: changing attitudes to Venice in late Byzantium
Authors: Leonte Florin 
Published: February 2025

AbstractThis paper explores the changing attitudes towards Venice in late Byzantine texts. It argues that, along with the strengthening of political and cultural ties between Byzantium and Venice, the Byzantines' perspectives evolved from rejection to admiration. As scholars like Demetrios Kydones and Manuel Chrysoloras began to teach Greek in Venice, exchanges intensified and the Byzantines' views became more nuanced. The later Byzantine émigrés in Italy, George of Trebizond and Bessarion, not only fostered stronger intellectual links but also perceived the Republic as supporting their efforts to reinvent a Hellenic identity outside Byzantium. While court rhetoric collapsed Venice's representations into a generic image of Italy, historians like Laonikos Chalkokondyles idealized its political system whose roots, he emphasized, were to be found in the ancient political systems. Finally, after 1453, vernacular laments of the fall of Constantinople, portrayed Venice as inheritor and potential saviour of Byzantium's imperial glory, thereby signalling the connections between the cultivation of the Byzantine heritage and Venice's political ambitions.
Posting environmental risk: Communicating with numerical and nonnumerical messaging across information modalities
Authors: Norman Megan L. P. , Bao Jiaqi Agnes , Kurniasari Triwik , Skurka Chris 
Published: February 2025

AbstractRisk communicators frequently engage members of the public through social media; therefore, it is important to understand how to effectively communicate risks about environmental and health issues. This experiment with U.S. adults (N = 737) explored how message features in risk‐focused social media posts—namely, message modality (text‐only posts or text with the inclusion of photographs or data presentations) and risk representation (numerical risk details or nonnumerical summaries of risk information)—influence risk perceptions, perceived message informativeness, and intentions to share the content. To do so, we drew from research on visual communication and research on numerical and nonnumerical representations of risk. We assessed the robustness of these effects across three environmental risk domains (asthma, heat waves, and vector‐borne diseases). We found that compared to text‐only messages, text with a data presentation (i.e., graph or table) led to enhanced risk perceptions, perceptions of informativeness, and sharing intentions. Relative to nonnumerical messages, numerical messages generated greater perceived risk, were rated as more informative, and elicited greater sharing intentions. Additionally, the effects of risk representation depended on participants’ numeracy level, such that numerical messages (vs. nonnumerical) elicited greater sharing intentions and perceived informativeness among high‐numeracy individuals but had no such effects for low‐numeracy individuals. We also found some evidence that message effects on perceived informativeness differed based on risk topic. Our results suggest that communicators can consider designing social media risk messages with numerical details and data visualizations.
The Novel HLA‐A*02:1195N Null Allele Identified by Next‐Generation Sequencing
Authors: Schmitt Justine , Gothot André 
Published: February 2025

ABSTRACTHLA‐A*02:1195N differs from HLA‐A*02:01:01:01 by one nucleotide substitution in exon 4, at gDNA position 1843 (G>A).
Characterisation of the Novel HLA‐DQB1*05:354 Allele by Sequencing‐Based Typing
Authors: Pajot Thibault , Elsermans Vincent , Top Isabelle , Labalette Myriam 
Published: February 2025

ABSTRACTHLA‐DQB1*05:354 differs from HLA‐DQB1*05:01:01:01 by one nucleotide substitution in codon 55 in exon 2.
Widespread Involvement of Low‐Abundance KREEP in the Mantle of Chang'e‐5 and Its Surrounding Units
Authors: Li Ziqing , Zhang Bo , Wang Shui‐jiong , Che Xiaochao , Long Tao 
Published: February 2025

ABSTRACTThe return of the young Chang'e‐5 (CE‐5) samples provide new insights into the lunar thermal evolution. In this study, we present a new type of exotic low‐Ti basalt in CE‐5 breccia, which has a magmatic equigranular texture with low Ti content and high Mg bulk composition and minerals. Low rare earth elements (REEs) imply that the clast was probably derived from a primitive source, which may originate from western ancient mare unit. Based on fractional crystallisation modelling, the exotic basalt source is considered as low‐Ti high‐Al, opposing the source of CE‐5 basalts. Our partial melting and REE modelling further imply that the addition of 8% plagioclase and only 1% KREEP contributes to the formation of this specific magma source. This low degree of KREEP involvement in mantle sources may be widespread in Chang'e‐5 terrane and its surrounding units, playing an important role in prolonging lunar magmatism.
PcloC‐Mediated Phase Separation in Short‐Distance Vesicle Transport
Authors: Yan Huyong , Deng Yixuan 
Published: February 2025

ABSTRACTPhase separation is increasingly recognized as a paradigm to elucidate the self‐assembly and organization of membrane‐less bodies within the cell, which involves the segregation of a multi‐component system into distinct phases with varying compositions and structures. The latest study has found that protein aggregates formed through phase separation can effectively realize short‐distance transport of vesicles. PcloC responds to calcium through C2A domain‐mediated calcium sensing, thereby extracting synaptic vesicles from the reserve pool where synaptic proteins aggregate into the surface of the active zone protein condensate. Therefore, PcloC‐mediated phase separation may provide a new perspective to understanding short‐distance directional transport within cells.
Needle tenoscopy of the digital flexor tendon sheath in a standing equine cadaver model using a novel approach and guided palmar/plantar annular ligament desmotomy
Authors: Breen Louise J. , Stack John D. , Gillen Alex M. , Baldwin Chris M. 
Published: February 2025

AbstractObjective(1) To evaluate the feasibility of needle tenoscopy of the digital flexor tendon sheath (DFTS) using basisesamoid (BS) and proximolateral (PL) approaches in a standing equine cadaver model. (2) To report visualization of intrathecal DFTS anatomy via both approaches. (3) To determine the efficacy of needle scope‐guided palmar/plantar annular ligament (PAL) desmotomy. (4) To report any iatrogenic damage associated with the procedure.Study designEx vivo experimental.Sample populationTen equine cadaver limbs.MethodsLimbs were placed in a Kimzey leg‐saver splint and needle tenoscopy was performed using the BS and PL approaches. Two European College of Veterinary Surgeons (ECVS) Diplomates assessed and categorized intrathecal site visualization as poor, partial, or excellent. Needle scope‐guided PAL desmotomy was performed after DFTS exploratory needle tenoscopy. Limbs were dissected and examined for the presence of iatrogenic damage and completeness of PAL desmotomy. A Wilcoxon signed‐rank test was used to compare visualization scores for both approaches.ResultsNeedle tenoscopy of the DFTS in a standing model was feasible from both BS and PL approaches. Excellent visualization of clinically significant intrathecal anatomy within the fetlock canal was achieved from both approaches (p ≤ .001), with minimal iatrogenic damage. The PL approach allowed more structures to be visualized than the BS approach (p = .025). All PAL desmotomies were completed without associated iatrogenic damage.ConclusionNeedle tenoscopy of the DFTS in a standing model provided excellent visualization of intrathecal sites within the fetlock canal. It facilitated complete PAL desmotomy.Clinical significanceNeedle ten0oscopy can be used to assess the DFTS and to guide PAL desmotomy in a standing horse.
Transplant Patients’ Perceptions About Participating in First‐in‐Human Pig Kidney Xenotransplant Clinical Trials: A Mixed Methods Study
Authors: Gordon Elisa J. , Maschke Karen J. , Gacki‐Smith Jessica , Brooks Hannah L. , Matthews Margaret M. , Traboulsi Karen , Manning Dahlya , Leventhal Joseph , Gusmano Michael K. 
Published: February 2025

ABSTRACTFirst‐in‐human pig xenotransplant clinical trials may soon begin, which raises ethical concerns about patients’ decision‐making to participate in such trials. We assessed kidney transplant candidates’ attitudes and hypothetical decision‐making about participating in xenotransplant trials through semi‐structured telephone interviews and an online survey. We analyzed qualitative data by thematic analysis and quantitative data by descriptive statistics. Twenty‐eight patients participated in interviews; 142 patients participated in the survey. Most interview and survey respondents were male (68%, 56%), White (54%, 61%), or Black (36%, 22%). Although interview participants appreciated xenotransplantation research's potential to advance science, they expressed concerns about infection transmission and graft function. Few survey respondents were willing to participate in a pig kidney trial to test the safety of pig kidneys (12.6%) or pig kidney function (16.9%). Interview participants would be more likely to participate in a first‐in‐human pig kidney trial if receiving a human kidney was unlikely and their health status declined. Willingness would also depend on how long the pig kidney would function. Most interview participants were receptive to long‐term monitoring, but not to family monitoring. Transplant programs planning xenotransplant trials should anticipate these types of concerns for optimizing human subject protections and conducting a robust informed consent process.
A Brief Review on the Tribological Effects of Electrically Induced Bearing Damage
Authors: Notay Rai Singh 
Published: February 2025

Abstract
Over the past two decades, the phenomenon of electrical stray currents in bearings has garnered significant attention from researchers and industry professionals. With the widespread adoption of electric motors and generators in applications like electric vehicles and sustainable energy sources, understanding and mitigating the potential bearing damage caused by these currents has become paramount. This paper provides a concise review of the sources of electrical bearing damage, failure modes, and their implications for critical tribological factors such as bearing life and lubrication. Special emphasis is placed on the challenges posed by elastohydrodynamic lubrication in the presence of electrical potential and the impact on durability, highlighting the need for further research. The paper concludes by emphasizing the importance of collaboration among original equipment manufacturers (OEMs), tier suppliers, lubrication additive companies, and academic institutions in advancing our understanding and effectively addressing electrically induced bearing damage. By exploring this complex topic, this paper aims to contribute to the broader scientific knowledge base and inspire future investigations in this field.