bluuebunny/crossref_metadata_2025_split huggingface dataset:
The whole transcriptomic analysis of local bone tissue after fracture
Authors: Wang Shen , Guo Shuhang , Yuan Shaoyun , Gu Xinyi , Deng Jin , Zeng Xinyi , Lu Qingguo , Yin Xiaofeng , Huang Chen 
Published: March 2024

Abstract
Fracture healing is a complex physiological process involving multiple cells and signaling pathways, the potential molecular mechanisms and biological process are still unclear and need further exploration. In this study, transcriptome sequencing technology was used to detect and analyze the changes in transcriptome of the local injury tissue after fracture. Differentially expressed genes (DEGs) with the high degree were analyzed. Our results showed that different stages of fracture healing had different focuses, some important biological processes, such as the inflammatory response, mainly occurred on the 3rd day after fracture. Besides, we found that the 3rd day after fracture was a key point of transcriptome change, and neural regulation played a significant role in fracture healing at this time, and inflammatory stimulation might be an important factor affecting neural regulation after fracture. In conclusion, our research results identify some important genes and pathways in the process of fracture healing, and summarize the main characteristics of transcriptome changes in fractures. This laid the foundation for a deeper understanding of the relation between molecular mechanisms and biological processes involved in fracture healing. By identifying the specific time points at which various cellular events occur during the healing process, researchers can gain insights into the complex interplay between cells, signaling pathways, and extracellular matrix components that is required for successful bone healing. This knowledge can inform the development of new therapies and interventions to improve fracture healing outcomes and ultimately enhance the quality of life for patients affected by bone fractures.
Homologous 111In-radiolabeled platelet survival and sequestration exploration for refractory immunologic thrombocytopenic purpura in children: a strategy to avoid unnecessary splenectomy
Authors: Dubois Julien , Kucharczak Florentin , De Neef Léa , Kouyoumdjian Virginie , Palenzuela Gilles , Tunez Virginie , Mariano-Goulart Denis , Bourdon Aurélie , Paunet Tom 
Published: March 2024

Abstract
Purpose Immunologic thrombocytopenic purpura (ITP) is a condition that affects 4 to 18 per 100 000 children every year. In most cases, spontaneous remission occurs, but splenectomy can be proposed to diminish excessive destruction of platelets when ITP is refractory. Exploring the site of platelet sequestration may help to better predict potential poor responders to splenectomy if no spleen sequestration is highlighted. The radiolabeled platelet sequestration test may be an option but can be difficult to perform in children with very few platelets.
Methods A twelve-year-old boy suffering from chronical ITP was referred to evaluate platelet survival and sequestration, to discuss splenectomy. The patient was refractory to therapy and his platelet count consistently remained below 10 G.L-1. An exceptional procedure was set up to use homologous platelets to perform a radiolabeled platelet survival and sequestration test. A radiolabeled autologous platelet exam was carried five months later to confirm the test.
Results Significantly reduced platelet lifespan and half-life was observed for both techniques. The splenic and hepatic ratio were below 1.2 at all time points, meaning that there was no organ sequestration. The fact that we confirmed our test by two different methods allowed us to reject the indication for splenectomy in this child. Strict intravascular destruction has been confirmed. Conclusions 111In-radiolabeled homologous platelets were used to perform the examination and confirmed the results obtained with autologous platelets. Homologous platelet scintigraphy could help clinicians to choose splenectomy as a treatment option for ITP in children with very low platelet count. Work must be done to promote its use.
Engineered Bacterium DF4-8 Achieves Specific Phenol Detection via a unique mopR-like Regulatory Pathway
Authors: Abd-El-Haleem Desouky 
Published: March 2024

Abstract
The engineered Acinetobacter sp. strain DF4-8 demonstrates exceptional bioluminescent detection capabilities for phenol, showcasing its potential as a highly specific environmental biosensor. A novel mopR-like gene within DF4-8, distinct from known mopR genes, points to a unique regulatory pathway governing phenol-induced bioluminescence. Genomic analysis uncovers a complex mopR-like gene region in DF4-8, featuring distinct ORFs and promoters compared to its bioluminescence-negative counterpart, DF4-10. Each ORF exhibits a unique predicted protein structure and harbors novel motifs, with DF4-ORF8 notably carrying a discovered NAD-binding motif (EKRDQVLSLITATA). Co-factor analysis reveals a potential NAD-binding domain (QATKVGVLGAGMMGADVTKWQATKVGVLGAGMMGA) within DF4-ORF8, suggesting its dual functionality in metabolic pathways and phenol recognition. Further exploration of specific regulatory factors, such as ArgR for mopR-ORF, PhoB for DF4-ORF8, and RpoD17 for DF4-ORF10, is crucial for understanding this intricate regulatory network. The study also hints at a potential role of transposable elements (Tn elements) in explaining the dissimilarity among DF4-8, the mopR-like gene, and DF4-10 DNA sequences. These findings shed light on a previously undescribed regulatory pathway governing phenol-induced bioluminescence in DF4-8, unveiling a promising biosensor with enhanced detection specificity and opening avenues for further exploration of the intricate regulatory mechanisms within the mopR-like gene region.
Influences on improved confidence among allied health students in working with Australian Indigenous people during a rural placement: a pre-post survey study
Authors: Woods John A , Fitzgerald Kathryn , Papertalk Lennelle P , Green Charmaine , Rasiah Rohan L , Moran Monica , Bentink Samantha , Thompson Sandra C 
Published: March 2024

Abstract

Background: Together with addressing social determinants of health, culturally safe healthcare provision is essential for closing the health outcomes gap experienced by Aboriginal and Torres Strait Islander (Indigenous) Australians. Rural placements potentially provide students of the health professions with opportunities to enhance their knowledge and skills regarding cultural safety. We used rural placements data systematically collected from allied health students, including commencement- and end-of-placement questionnaire responses, to investigate the determinants of confidence in working with Indigenous people.
Methods: The study comprised data from all students who provided survey data at both commencement and end of their first placement directly supervised by the administering University Department of Rural Health during the period 2019–2022. Five-point ordered responses to the question 'How confident do you feel about working with Aboriginal people?' were used to assess student and placement-related determinants of confidence (Confident/Very confident versus other) at baseline and increased confidence (≥1 point) during the placement using crude and adjusted multivariable robust Poisson regression.
Results: Participating students (N=453) were from diverse allied health disciplines (including pharmacy n=92; 20.3%; chiropractic and social work each n=59, 13.0%; occupational therapy and physiotherapy each n=56, 12.4%). Confidence in dealing with Aboriginal people was higher at commencement among males compared with females (adjusted relative risk [aRR] 1.51; 95% confidence interval [CI] 1.22–1.86), students of Australian rural origin compared with others (aRR 1.46; CI 1.18–1.80), and those who reported previous experience working with Indigenous people (aRR 1.40; CI 1.13–1.72). Notably, for all three of these categories, complementary subgroup 'catch-up' during the placement was evident. Placement attributes associated with increased confidence working with Indigenous people between placement commencement and end were interaction with Indigenous people within the placement (aRR 2.15; CI 1.17–3.98), placement model reflecting more structured academic supervision (aRR 1.22; CI 1.04–1.43), and placement length (aRR per additional day 1.002; CI 1.001–1.004).
Conclusions: While influenced by students’ demographic attributes and prior experiences, confidence of allied health students in working with Indigenous people is enhanced during rural placements, particularly through direct contact with Indigenous people.
Spermine Synthase Engages in Macrophages M2 polarization to Sabotage Antitumor Immunity in Hepatocellular Carcinoma
Authors: Fang Yuan , Sun Yining , Zhou Peitao , Zeng Qin , Qian Junying , Wei guangyan , Li Yongsheng , Liu Yuechen , Lai Yingjie , Wang Wei , Zhan Yizhi , Wu Dehua 
Published: March 2024

Abstract

Disturbances in tumor cell metabolism reshape the tumor microenvironment (TME) and impair antitumor immunity, but the implicit mechanisms remain elusive. Here, we found that spermine synthase (SMS) was significantly upregulated in tumor cells, which correlated positively with immunosuppressive microenvironments and predicted poor survival in hepatocellular carcinoma (HCC) patients. Via “subcutaneous” and “orthotopic” HCC syngeneic mouse models and a series of in vitro coculture experiments, we identified elevated SMS level in HCC cells played a role in immune escape mainly through its metabolic product spermine, which induced tumor-associated macrophage (TAM) reprogramming and subsequently corresponded with a decreased antitumor functionality of CD8+ T cells. Mechanistically, we discovered that spermine reprogrammed TAM mainly by activating the PI3K-Akt-mTOR-S6K signaling pathway. Spermine inhibition in combination with immune checkpoint blockade effectively diminishes tumor burden in vivo. Our results expand the understanding of the critical role of metabolites in regulating cancer progression and anti-tumor immunity, and open new avenues for developing novel therapeutic strategies against HCC.
Associations Between Cerebrospinal Fluid Synaptic Protein Biomarkers and Cognitive Function in Bipolar Disorder
Authors: Knorr Ulla , Simonsen Anja , Nilsson Johanna , Brinkmalm Ann , Blennow Kaj , Zetterberg Henrik , Knudsen Mark , Forman Julie , Miskowiak K , Hasselbalch Steen , Kessing Lars 
Published: March 2024

Abstract

This study is the first to investigate the association between a comprehensive panel of cerebrospinal fluid (CSF) synaptic protein biomarkers and cognitive function utilizing data from a prospective cohort study including N = 59 patients with bipolar disorder (BD) and N = 37 healthy control individuals (HC). Biomarkers of synaptic dysfunction were analyzed by targeted mass spectrometry. They included neuronal pentraxin (NPTX)1, NPTX2, 14-3-3 proteins, AP-2 complex subunit-beta, beta-synuclein, complexin-2, gamma-synuclein, NPTX receptor, phosphatidylethanolamine-binding proteins, rab GDP dissociation inhibitor-alpha, syntaxins-1B and 7. The primary cognition measure was a global cognitive composite score based on neuropsychological tests probing verbal learning and memory, executive function, psychomotor speed, and sustained attention, respectively. Our primary hypothesis was that levels of NPTX1 and NPTX2 were associated with global cognition and verbal memory after adjustment for age, sex, and follow-up time as potential confounders. The study revealed consistent positive associations between CSF protein concentrations of NPTX1 and NPTX2 and global cognitive function. However, only the association with a tryptic peptide from NPTX2 (VAELEDEK) remained statistically significant after adjustment for multiple testing. In contrast, no consistent trends and no significant relationships were found between NPTX1 and NPTX2 and the verbal learning and memory domain. In exploratory analyses, NPTXs showed positive associations with sustained attention, and the NPTX receptor showed a positive association with global cognition scores. Similar trends were found in BD patients and HC individuals, although results remain statistically uncertain, and a spurious finding cannot be ruled out due to multiple testing. In summary, the study provides novel evidence for a potentially pivotal role of CSF synaptic proteins, particularly NPTX1, NPTX2, and NPTX receptor, in shaping global cognitive function across BD and HC populations and increases our understanding of the neurobiological foundations for cognitive functions across diagnostic boundaries.
Steady motion of 80-nm-size skyrmions in a 100-nm-wide track
Authors: Du Haifeng , Song Dongsheng , Wang Weiwei , Zhang Shuisen , Wang Ning , Zheng Fengshan , Tian Mingliang , Dunin-Borkowski Rafal , Zang Jiadong 
Published: March 2024

Abstract

The current-driven movement of magnetic skyrmions along a nanostripe is essential for the advancement and functionality of a new category of spintronic devices resembling racetracks. Despite extensive research into skyrmion dynamics, experimental verification of current-induced motion of ultra-small skyrmions within an ultrathin nanostripe is still pending. Here, we unveil the motion of individual 80-nm-size skyrmions in an FeGe track with an ultrathin width of 100 nm. The skyrmions can move steadily along the track over a broad range of current densities by using controlled pulse durations of as low as 2 ns. The potential landscape, arising from the magnetic edge twists in such a geometrically-confined system, introduces skyrmion inertia and ensures efficient motion with a vanishing skyrmion Hall angle. Our results showcase the steady motion of skyrmions in an ultrathin track, offering a practical pathway for implementing skyrmion-based spintronic devices.
Different numbers of conjunctive stimuli induce LTP or LTD in mouse cerebellar Purkinje cell
Authors: Daida Atsuro , Kurotani Tohru , Yamaguchi Kazuhiko , Takahashi Yuji , Ichinohe Noritaka 
Published: March 2024

Abstract

Long-term depression (LTD) of synaptic transmission at parallel fiber (PF)-Purkinje cell (PC) synapses plays an important role in cerebellum-related motor coordination and learning. LTD is induced by the conjunction of PF stimulation and climbing fiber (CF) stimulation or somatic PC depolarization, while long-term potentiation (LTP) is induced by PF stimulation alone. Thus, it has been considered that different types of stimulation induce different types of synaptic plasticity. However, we found that a small number of conjunctive stimulations (PF + somatic depolarization) induced LTP, and not a small size of LTD. This LTP was not associated with changes in paired-pulse ratio and was dependent on nitric oxide, suggesting postsynaptic LTP. This LTP was also induced by a smaller number of physiological conjunctive stimulations, utilizing PF and CF stimulation. These results suggested that a larger number or longer period of conjunctive stimulation is required to induce LTD by overcoming LTP. Ca2+ imaging during physiological conjunctive stimulation at 0.5 Hz for 180 s (90 times), which was required for LTD induction, demonstrated that the peak amplitude of the Ca2+ signal at the PC dendritic region increased gradually, responding to the repetitive conjunctive stimulation. Instantaneous peak amplitude was not different between the early phase (50–60 s, 26th-30th) and late phase (170–180 s, 86th-90th), but the average amplitude for 60 s was larger in the later phase than in the early phase. These results show that LTD overcomes LTP, and increased Ca2+ integration or a number of stimulations is required for LTD induction.
Forecasting Air Quality in Peninsular Malaysia: Unveiling the Power of Artificial Neural Networks
Authors: SHAFI'I MOHD SUZAIRI MOHD , JUAHIR HAFIZAN 
Published: March 2024

Abstract

Air pollution poses significant risks to human health, the environment, and the economy. Therefore, striving for cleaner air through efficient air quality monitoring is imperative for fostering a healthier and more sustainable future. Predicting air quality is essential to enhance the quality of life, maintain environmental sustainability, and reduce the economic burden associated with poor air quality issues. The artificial neural network (ANN) is widely recognized as a predominant computational tool in air quality studies due to its capabilities in predicting gaseous and particulate pollutant concentrations, as well as forecasting the air pollutant index (API). This study aimed to investigate the predictive performance of ANN in determining the API by utilizing identified potential sources of air pollutants. Five prediction models were created, namely ANN-PC2018, ANN-PC2019, ANN-PC2020, ANN-PC2021, and ANN-PC2022. Principal component analysis (PCA) was conducted to identify the most significant sources of air pollution, and the results were employed to predict the API using ANN. The ANN-PC2019 model exhibited the highest performance with an R2 value of 0.8612 and RMSE of 7.7467, utilizing four major pollutants as input variables. These findings suggest that forecasting air quality using fewer parameters yields reliable outcomes.
Concurrent Transurethral Resection of The Prostate (TURP) and Inguinal Hernioplasty
Authors: Hsu Ting-Wei , Tseng Wen-Hsin , Huang Steven K. , Chiu Allen W. , Li Chien-Feng , Shiue Yow-Ling 
Published: March 2024

Abstract

Background
Benign prostatic hyperplasia (BPH) is a prevalent condition in aging males, leading to bladder outlet obstruction (BOO) and associated urinary symptoms. With increasing life expectancy, the incidence of BPH and its co-morbidities, like inguinal hernia, has risen. This study explores the efficacy of combining transurethral resection of the prostate (TURP) and inguinal hernioplasty in a single surgical session to address both conditions, potentially reducing the need for multiple hospitalizations and surgical interventions.
Methods
This retrospective study at Chi Mei Medical Center included patients from 2014 to 2023 who underwent concurrent TURP and inguinal hernioplasty. A total of 85 patients met the criteria defined for this study. Preoperative, intraoperative, and postoperative characteristics were meticulously documented. Outcomes evaluated included the duration of the surgery, incidence of intraoperative and postoperative complications, duration of Foley catheterization, length of hospital stay, and treatment efficacy. Additionally, we conducted a comparative assessment of the surgical outcomes between two distinct techniques for inguinal hernia repair: open hernioplasty and laparoscopic hernioplasty (LH).
Results
In 85 patients who met the criteria, the mean age was 71.1 ± 7.8 years. The study reported no significant intraoperative complications, and postoperative care was focused on monitoring for blood loss, infection, and managing pain. The average postoperative hospital stay was 2.9 ± 1.0 days and the mean duration of catheterization was 51.6 ± 16.7 hours, with a minimal complication rate observed during the one-year follow-up. A significant reduction in both operative duration and catheterization interval was observed in patients undergoing LH as opposed to those receiving open hernioplasty.
Conclusion
Concurrent TURP and inguinal hernioplasty effectively manage BOO due to BPH and inguinal hernias with minimal complications, suggesting a viable approach to reducing hospital stays and surgical interventions. Laparoscopic techniques, in particular, offer benefits in operative efficiency and recovery time, making combined surgery a feasible option for selected patients.
The molecular mechanisms of imatinb treatment on acute lung injury in septic mice through proteomic technology
Authors: Wang Xiao Wang , Zhou ZhiQing Zhou , Li DuanYang Li , Zhang BoYang Zhang , Zong XiaoLong Zong , Li ZhenYu Li , Liang Xue Liang 
Published: March 2024

Abstract
Background
Acute lung injury (ALI) is the most common complication of sepsis. Despite considerable progress in the treatment of sepsis, morbidity and mortality associated with sepsis-induced ALI remain high and effective treatment strategies are lacking. A previous study has shown that imatinib reduces the rate of acute pulmonary damage in septic mice, and this particular study was ongoing for more than a decade. However, the molecular mechanism by which imatinib reduces lung injury in septic mice remains unclear. Therefore, the current study aimed to investigate the potential mechanism by which imatinib alleviates ALI in septic mice.
Methods
A septicemia model was established by intraperitoneal injection of lipopolysaccharide (LPS), followed by tail vein injection of imatinib in the treatment group and normal saline injection in the control group. Flow cytometry was used to detect inflammatory factors, and hematoxylin staining was used to detect pathological injury to the lung tissue. TMT quantitative labeling technology was used for proteomic sequencing analysis. The main target protein was identified through bioinformatics and its expression was confirmed using western blotting.
Results
We identified 706 differentially expressed proteins, 128 of which were associated with the protective effects of imatinib against septic lung injury. Functional enrichment analysis indicated that imatinib-related differentially expressed proteins may be related to electron transfer, coagulation, and endothelial cell regulation in the oxidative respiratory chain. Enrichment of the nuclear factor-kappa B (NF-kB) signaling pathway, complement-coagulation cascade, chemokine signaling pathway, and cytochrome-cytochrome receptor interaction pathway were also observed. Additionally, we found that the expression of CCAAT/enhancer-binding protein delta (CEBPD) and pyruvate dehydrogenase kinase 4 (PDK4) increased in the sepsis group, but decreased in the imatinib group, consistent with our sequencing results.
Conclusion
Imatinib may reduce ALI in mice with sepsis by participating in oxidative respiratory and inflammatory responses, clotting response-related signaling pathways, and downregulating CEBPD and PDK4 expression.
Design of an electrospun polyvinyl alcohol (PVA)/chitosan/curcumin based- wound dressing containing titania nanotubes (TNT)
Authors: Eslami Hossein , Kabiri Naiemeh , Ansari Mojtaba , Zare-Zardini Hadi , Pisheh Hossein Rayat 
Published: March 2024

Abstract
Burn wounds are one of the most significant causes of morbidity around the world. Burn wound healing and skin tissue regeneration still have serious and considerable challenges. This study applies the electrospinning method to make PVA-chitosan-curcumin wound dressing containing titania nanotubes with 0.5, 1.5, and 3 wt%, following that their physicochemical properties were investigated using scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and mechanical test. Then, the in vivo effectiveness of the designed wound dressing in the wound healing process in the Wistar rat model was evaluated for two weeks. The most important result of applying the TiO2 nanotube was the improved regeneration of damaged tissues with an appreciable decline in scar formation, skin color anomalies, and accelerated wound healing and contraction.