bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocol for a systematic review of the management and outcomes in patients with gallbladder agenesis
Authors: Young Erin N , Dongho Ghyslaine B D , Ngo Ngo Valery , Asahngwa Constantine , Jasmin Hilary M , Nelson John S , Zalamea Nia N , Gobina Ronald M , Foretia Denis A 
Published: February 2024

Abstract
Gallbladder agenesis (GA) is a congenital anomaly of the biliary system, with characteristic absence of the gallbladder and cystic duct often with variable clinical presentation. Despite major advances in imaging technology many patients undergo unnecessary operative interventions during which the diagnosis is made. Very little is known on the management and outcomes in this patient population. Therefore, the main objective of this study protocol is to guide a comprehensive review of published cases of GA to evaluate and synthesize research findings on its presentation, management and clinical outcomes. This systematic review will be conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols guidelines. MEDLINE, via PubMed, EMBASE, SCOPUS and Google Scholar will be searched for articles on GA in humans (case report and case series) published before 1 October 2022 in English, French and Spanish. Two independent investigators will review each article, determine if it meets inclusion criteria, extract data and assess the study quality. A third investigator will arbitrate studies where there are conflicts between the first two investigators. Short and long-term outcomes will be compared between cases that underwent non-operative versus open versus laparoscopic surgery. Estimates will be made of odds ratios for dichotomous outcomes.
Evidence for dual targeting control of Arabidopsis 6-phosphogluconate dehydrogenase isoforms by N-terminal phosphorylation
Authors: Doering Lennart Nico , Gerling Niklas , Linnenbrügger Loreen , Lansing Hannes , Baune Marie-Christin , Fischer Kerstin , von Schaewen Antje 
Published: February 2024

Abstract
The oxidative pentose-phosphate pathway (OPPP) retrieves NADPH from glucose-6-phosphate, which is important in chloroplasts at night and in plastids of heterotrophic tissues. We previously studied how OPPP enzymes may transiently locate to peroxisomes, but how this is achieved for the third enzyme remained unclear. By extending our genetic approach, we demonstrated that Arabidopsis isoform 6-phosphogluconate dehydrogenase 2 (PGD2) is indispensable in peroxisomes during fertilization, and investigated why all PGD–reporter fusions show a mostly cytosolic pattern. A previously published interaction of a plant PGD with thioredoxin m was confirmed using Trxm2 for yeast two-hybrid (Y2H) and bimolecular fluorescent complementation (BiFC) assays, and medial reporter fusions (with both ends accessible) proved to be beneficial for studying peroxisomal targeting of PGD2. Of special importance were phosphomimetic changes at Thr6, resulting in a clear targeting switch to peroxisomes, while a similar change at position Ser7 in PGD1 conferred plastid import. Apparently, efficient subcellular localization can be achieved by activating an unknown kinase, either early after or during translation. N-terminal phosphorylation of PGD2 interfered with dimerization in the cytosol, thus allowing accessibility of the C-terminal peroxisomal targeting signal (PTS1). Notably, we identified amino acid positions that are conserved among plant PGD homologues, with PTS1 motifs first appearing in ferns, suggesting a functional link to fertilization during the evolution of seed plants.
Biomaterial design for regenerating aged bone: materiobiological advances and paradigmatic shifts
Authors: Dai Kai , Geng Zhen , Zhang Wenchao , Wei Xue , Wang Jing , Nie Guangjun , Liu Changsheng 
Published: February 2024

ABSTRACT
China's aging demographic poses a challenge for treating prevalent bone diseases impacting life quality. As bone regeneration capacity diminishes with age due to cellular dysfunction and inflammation, advanced biomaterials-based approaches offer hope for aged bone regeneration. This review synthesizes materiobiology principles, focusing on biomaterials that target specific biological functions to restore tissue integrity. It covers strategies for stem cell manipulation, regulation of the inflammatory microenvironment, blood vessel regeneration, intervention in bone anabolism and catabolism, and nerve regulation. The review also explores molecular and cellular mechanisms underlying aged bone regeneration and proposes a database-driven design process for future biomaterial development. These insights may also guide therapies for other age-related conditions, contributing to the pursuit of ‘healthy aging’.
Combination of Extended Antivirals With Antiretrovirals for Severe Mpox in Advanced Human Immunodeficiency Virus Infection: Case Series of 4 Patients
Authors: Duong Michael T , Tebas Pablo , Ancha Bhavya , Baron Jillian , Chary Pallavi , Isaacs Stuart N , Szep Zsofia 
Published: February 2024

Abstract
To gauge the safety and utility of extended tecovirimat/cidofovir for severe mpox, here we report our experience caring for 4 patients with mpox and advanced human immunodeficiency virus (HIV) at the Hospitals of the University of Pennsylvania during the 2022 global outbreak. Three patients had recurrent courses complicated by superinfections, coinfections and insufficient nutrition/housing, requiring extended tecovirimat (5–16 weeks) and cidofovir (1–12 doses) with probenecid and fluids. At follow-up, patients had undetectable HIV RNA on antiretrovirals, improved ulcers and stable renal function on antivirals. Serology guided cessation for one 7-month cidofovir course. Overall findings support a comprehensive approach of prolonged tecovirimat/cidofovir with antiretrovirals for severe mpox, while addressing social factors.
The “four Ds” and support for Local Smart Grids: analysis from national surveys in the UK and Canada
Authors: Walker Chad , Rowlands Ian H , Devine-Wright Patrick , Soutar Iain , Wilson Charlie , Gupta Rajat , Devine-Wright Hannah , Bishwokarma Juli , Ford Rebecca 
Published: February 2024

AbstractLocal Smart Grids are emerging during the climate crisis, as governments and industry recognize the need to better integrate intermittent renewable energy, storage, transportation, heating, and smart technologies. Such projects can represent profound changes to the status quo of energy and citizen lifestyles. They are also being associated with the “four Ds,” whereby Local Smart Grids are decarbonizing, decentralizing, digitalizing, and potentially democratizing energy systems. Yet, due to their recent arrival, there is very little social scientific research that has aimed to better understand public views, expectations, and support for this change. We attempt to fill this important gap in the literature through the analysis of two nationally representative surveys in the UK (n = 3034) and Canada (n = 941). This analysis highlights within- and between-country trends, including how the variation in responses regarding the “four Ds,” demographic factors, and other variables may explain the differences we see in terms of support for energy system change in the UK and Canada. Our analysis also shows that there are common elements, including the importance of the decentralization, and especially the democratization of energy in shaping support. We hope that this study will help governments, industry, community groups, and local residents themselves in both countries come together to advance the kind of Local Smart Grids that address climate change and represent a supported, just energy transition.
Dynamics of nanocluster aerosol in the indoor atmosphere during gas cooking
Authors: Patra Satya S , Jiang Jinglin , Ding Xiaosu , Huang Chunxu , Reidy Emily K , Kumar Vinay , Price Paige , Keech Connor , Steiner Gerhard , Stevens Philip , Jung Nusrat , Boor Brandon E 
Published: February 2024

Abstract
Nanocluster aerosol (NCA: particles in the size range of 1–3 nm) are a critically important, yet understudied, class of atmospheric aerosol particles. NCA efficiently deposit in the human respiratory system and can translocate to vital organs. Due to their high surface area-to-mass ratios, NCA are associated with a heightened propensity for bioactivity and toxicity. Despite the human health relevance of NCA, little is known regarding the prevalence of NCA in indoor environments where people spend the majority of their time. In this study, we quantify the formation and transformation of indoor atmospheric NCA down to 1 nm via high-resolution online nanoparticle measurements during propane gas cooking in a residential building. We observed a substantial pool of sub-1.5 nm NCA in the indoor atmosphere during cooking periods, with aerosol number concentrations often dominated by the newly formed NCA. Indoor atmospheric NCA emission factors can reach up to ∼1016 NCA/kg-fuel during propane gas cooking and can exceed those for vehicles with gasoline and diesel engines. Such high emissions of combustion-derived indoor NCA can result in substantial NCA respiratory exposures and dose rates for children and adults, significantly exceeding that for outdoor traffic-associated NCA. Combustion-derived indoor NCA undergo unique size-dependent physical transformations, strongly influenced by particle coagulation and condensation of low-volatility cooking vapors. We show that indoor atmospheric NCA need to be measured directly and cannot be predicted using conventional indoor air pollution markers such as PM2.5 mass concentrations and NOx (NO + NO2) mixing ratios.
Containing misinformation: Modeling spatial games of fake news
Authors: Jones Matthew I , Pauls Scott D , Fu Feng 
Published: February 2024

Abstract
The spread of fake news on social media is a pressing issue. Here, we develop a mathematical model on social networks in which news sharing is modeled as a coordination game. We use this model to study the effect of adding designated individuals who sanction fake news sharers (representing, for example, correction of false claims or public shaming of those who share such claims). By simulating our model on synthetic square lattices and small-world networks, we demonstrate that social network structure allows fake news spreaders to form echo chambers and more than doubles fake news’ resistance to distributed sanctioning efforts. We confirm our results are robust to a wide range of coordination and sanctioning payoff parameters as well as initial conditions. Using a Twitter network dataset, we show that sanctioners can help contain fake news when placed strategically. Furthermore, we analytically determine the conditions required for peer sanctioning to be effective, including prevalence and enforcement levels. Our findings have implications for developing mitigation strategies to control misinformation and preserve the integrity of public discourse.
COVID-19 Spillover Effects onto General Vaccine Attitudes
Authors: Lunz Trujillo Kristin , Green Jon , Safarpour Alauna , Lazer David , Lin Jennifer , Motta Matthew 
Published: February 2024

Abstract
Even amid the unprecedented public health challenges attributable to the COVID-19 pandemic, opposition to vaccinating against the novel coronavirus has been both prevalent and politically contentious in American public life. In this paper, we theorize that attitudes toward COVID-19 vaccination might “spill over” to shape attitudes toward “postpandemic” vaccination programs and policy mandates for years to come. We find this to be the case using evidence from a large, original panel study, as well as two observational surveys, conducted on American adults during the pandemic. Specifically, we observe evidence of COVID-19 vaccine spillover onto general vaccine skepticism, flu shot intention, and attitudes toward hypothetical vaccines (i.e., vaccines in development), which do not have preexisting attitudinal connotations. Further, these spillover effects vary by partisanship and COVID-19 vaccination status, with the political left and those who received two or more COVID-19 vaccine doses becoming more provaccine, while the political right and the unvaccinated became more anti-vaccine. Taken together, these results point to the salience and politicization of the COVID-19 vaccine impacting non-COVID vaccine attitudes. We end by discussing the implications of this study for effective health messaging.
Bilingual Statutory Interpretation and the United Kingdom: Domestic Law and International Experiences
Authors: Huws Catrin Fflûr 
Published: February 2024

Abstract
This article explores the emerging need for bilingual statutory interpretation within the United Kingdom and explains the significance of the High Court and Court of Appeal judgments in R (on behalf of Driver) v Rhondda Cynon Tâf County Borough Council both in terms of the process of statutory interpretation and with regard to its potential impact on the future of England and Wales as a unified jurisdiction. It draws upon the UK’s approaches to multilingual obligations under international law, and on comparative experiences of multilingualism within a domestic legal system.
TRIP6 promotes neural stem cell maintenance through YAP‐mediated Sonic Hedgehog activation
Authors: Li Ming‐Yang , Yang Xiu‐Li , Chung Chia‐Chi , Lai Yun‐Ju , Tsai Jui‐Cheng , Kuo Ya‐Lin , Yu Jenn‐Yah , Wang Tsu‐Wei 
Published: February 2024

AbstractIn the adult mammalian brain, new neurons are continuously generated from neural stem cells (NSCs) in the subventricular zone (SVZ)‐olfactory bulb (OB) pathway. YAP, a transcriptional co‐activator of the Hippo pathway, promotes cell proliferation and inhibits differentiation in embryonic neural progenitors. However, the role of YAP in postnatal NSCs remains unclear. Here, we showed that YAP was present in NSCs of the postnatal mouse SVZ. Forced expression of Yap promoted NSC maintenance and inhibited differentiation, whereas depletion of Yap by RNA interference or conditional knockout led to the decline of NSC maintenance, premature neuronal differentiation, and collapse of neurogenesis. For the molecular mechanism, thyroid hormone receptor‐interacting protein 6 (TRIP6) recruited protein phosphatase PP1A to dephosphorylate LATS1/2, therefore inducing YAP nuclear localization and activation. Moreover, TRIP6 promoted NSC maintenance, cell proliferation, and inhibited differentiation through YAP. In addition, YAP regulated the expression of the Sonic Hedgehog (SHH) pathway effector Gli2 and Gli1/2 mediated the effect of YAP on NSC maintenance. Together, our findings demonstrate a novel TRIP6‐YAP‐SHH axis, which is critical for regulating postnatal neurogenesis in the SVZ‐OB pathway.
ChatGPT as a Source of Patient Information for Lumbar Spinal Fusion and Laminectomy
Authors: Nian Patrick P. , Saleet Jayson , Magruder Matthew , Wellington Ian J. , Choueka Jack , Houten John K. , Saleh Ahmed , Razi Afshin E. , Ng Mitchell K. 
Published: February 2024

Study Design:
Retrospective Observational Study

Objective:
The objective of this study was to assess the utility of ChatGPT, an artificial intelligence chatbot, in providing patient information for lumbar spinal fusion and lumbar laminectomy in comparison with the Google search engine.

Summary of Background Data:
ChatGPT, an artificial intelligence chatbot with seemingly unlimited functionality, may present an alternative to a Google web search for patients seeking information about medical questions. With widespread misinformation and suboptimal quality of online health information, it is imperative to assess ChatGPT as a resource for this purpose.

Methods:
The first 10 frequently asked questions (FAQs) related to the search terms “lumbar spinal fusion” and “lumbar laminectomy” were extracted from Google and ChatGPT. Responses to shared questions were compared regarding length and readability, using the Flesch Reading Ease score and Flesch-Kincaid Grade Level. Numerical FAQs from Google were replicated in ChatGPT.

Results:
Two of 10 (20%) questions for both lumbar spinal fusion and lumbar laminectomy were asked similarly between ChatGPT and Google. Compared with Google, ChatGPT’s responses were lengthier (340.0 vs. 159.3 words) and of lower readability (Flesch Reading Ease score: 34.0 vs. 58.2; Flesch-Kincaid grade level: 11.6 vs. 8.8). Subjectively, we evaluated these responses to be accurate and adequately nonspecific. Each response concluded with a recommendation to discuss further with a health care provider. Over half of the numerical questions from Google produced a varying or nonnumerical response in ChatGPT.

Conclusions:
FAQs and responses regarding lumbar spinal fusion and lumbar laminectomy were highly variable between Google and ChatGPT. While ChatGPT may be able to produce relatively accurate responses in select questions, its role remains as a supplement or starting point to a consultation with a physician, not as a replacement, and should be taken with caution until its functionality can be validated.
A Retrospective Comparative Analysis on the Effect of Tranexamic Acid to Reduce Perioperative Blood Loss in Patients Undergoing Cervical Spine Surgery
Authors: Money Adam J. , Dumont Guillaume , Sheppard Sean , Jackson J. Benjamin , Spitnale Michael , Bakaes Yianni , Gauthier Chase , Grabowski Gregory 
Published: February 2024

Study Design:
Retrospective Cohort Study

Objective:
The purpose of this study is to assess the impact of intravenous TXA on blood loss outcomes in anterior, posterior, and combined approaches for elective cervical spine surgery.

Summary of Background Data:
Tranexamic acid (TXA) has been shown to reduce blood loss in a variety of operations, such as lumbar spine surgery. However, limited studies have evaluated the efficacy of TXA in cervical spine surgery.

Methods:
We performed a retrospective review of a single surgeon’s elective cervical spine operations between September 2011 and March 2017. Patients were divided into 3 groups: anterior approach, posterior approach, or combined approach. Patients were then further subdivided into TXA versus control groups based on whether they received TXA treatment. We performed multiple linear regressions to assess the relationship between the use of TXA and other dependent variables (number of vertebral levels treated, need for a vertebral corpectomy) on total perioperative blood loss, intraoperative estimated blood loss, postoperative drain output, total operative time, postoperative change in hemoglobin, and occurrence of transfusion and/or postoperative deep venous thrombus (DVT).

Results:
We found that the use of TXA statistically significantly reduced total perioperative blood loss (P=0.04) and postoperative drain output (P=0.004) in posterior surgical approach cervical spine surgery but did not statistically significantly impact any blood loss variables in anterior or combined surgical approaches to elective cervical spine surgery. The use of TXA was a significant predictor for a decrease in intraoperative (P=0.02) and postoperative (P<0.01) blood loss.

Conclusions:
This study found that TXA statistically significantly decreased total blood loss and postoperative drain output when controlling for multiple confounding factors.

Level of Evidence:
Level III.
Elective Single-Level Primary Anterior Cervical Decompression and Fusion for Degenerative Spondylotic Cervical Myelopathy Is Associated With Decreased Resource Utilization Versus Posterior Cervical Decompression and Fusion
Authors: Du Jerry Y. , Shafi Karim , Blackburn Collin W. , Chapman Jens R. , Ahn Nicholas U. , Marcus Randall E. , Albert Todd J. 
Published: February 2024

Study Design:
Retrospective cohort study.

Objective:
To compare elective single-level anterior cervical discectomy and fusion (ACDF) versus posterior cervical decompression and fusion (PCDF) for degenerative cervical myelopathy (DCM) in terms of (1) cost, (2) length of hospital stay, and (3) discharge destination in Medicare patients. A sub-analysis of potential cost drivers was also performed.

Background:
In the era of value-based medicine, there is substantial interest in reducing the cost of care. Both ACDF and PCDF are used to treat DCM but carry different morbidity and risk profiles that can impact hospital resource utilization. However, this has not been assessed on a national level.

Methods:
Patients undergoing single-level elective ACDF and PCDF surgery were identified using the 2019 Medicare Provider Analysis and Review (MedPAR) Limited Data Set (LDS) and Centers for Medicare and Medicaid Services (CMS) 2019 Impact File. Multivariate models of hospital cost of care, length of stay, and discharge destination were performed, controlling for confounders. A univariate sub-analysis of 9 revenue centers was performed.

Results:
In all, 3942 patients met the inclusion criteria. The mean cost of elective single-level cervical fusion for myelopathy was $18,084±10,783, and the mean length of stay was 2.45±2.95 d. On multivariate analysis, ACDF was independently associated with decreased cost of $5,814 (P<0.001), shorter length of stay by 1.1 days (P<0.001), and decreased risk of nonhome discharge destination by 58% (adjusted odds ratio: 0.422, P<0.001).
On sub-analysis of 9 revenue centers, medical/surgical supply ($10,497, 44%), operating room charges ($5401, 23%), and accommodations ($3999, 17%) were the largest drivers of charge differences.

Conclusions:
Single-level elective primary ACDF for DCM was independently associated with decreased cost, decreased hospital length of stay, and a lower rate of nonhome discharge compared with PCDF. Medical and surgical supply, operating room, and accommodation differences between ACDF and PCDF are potential areas for intervention. Increased granularity in reimbursement structures is warranted to prevent the creation of disincentives to the treatment of patients with DCM with pathology that is better addressed with PCDF.

Level of Evidence:
Level—III Retrospective Cohort Study.
Climacteric status at age 46 is associated with poorer work ability, lower 2-year participation in working life, and a higher 7-year disability retirement rate: a Northern Finland Birth Cohort 1966 study
Authors: Saarinen Tiia , Savukoski Susanna M. , Pesonen Paula , Vaaramo Eeva , Laitinen Jaana , Varanka-Ruuska Tuulia , Ala-Mursula Leena , Niinimäki Maarit 
Published: February 2024

Abstract

Objective
To study the association between an advanced climacteric status at 46 years of age and current perceived work ability, the consequent 2-year accumulation of disability and unemployment days, and the 7-year incidence of disability pensions.

Methods
Study participants (n = 2,661) were recruited from the Northern Finland Birth Cohort 1966 study's 46-year follow-up in 2012. The participants' perceived work ability was investigated using the Work Ability Score (0-7 = poor vs 8-10 = good), along with potential covariates. Data concerning their consequent disability days, unemployment days, and disability pensions were collected from national registers. The association between their climacteric status at age 46 years, work ability, and working life participation was assessed using regression models.

Results
The climacteric women were more often smokers and more often had a lower level of education. The odds ratio for poor perceived work ability was 1.41 (95% CI, 1.06-1.87), and the incidence rate ratios for disability and unemployment days during the 2-year follow-up were 1.09 (95% CI, 1.07-1.11) and 1.16 (95% CI, 1.14-1.18), respectively, for the climacteric women compared with the preclimacteric women in models adjusted for smoking and education. The 7-year hazard ratio for disability pensions was 1.72 (95% CI, 1.02-2.91) for the climacteric women.

Conclusions
An earlier menopausal transition is associated with poorer perceived work ability, and it predicts lower recorded work participation and a higher disability pension rate in subsequent years.