bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bullous congenital ichthyosiform erythroderma of Brocq
Authors: Kucharekova Martina , Mosterd Klara , Winnepenninckx Veronique , Van Geel Michel , Sommer Anja , Van Steensel Maurice A. M. 
Published: October 2007

AbstractBullous congenital ichthyosiform erythroderma (BCIE), also known as epidermolytic hyperkeratosis (EHK, OMIM 113800) is characterized by erythroderma and blistering at birth, leading to generalized hyperkeratosis of varying severity in adulthood. Clinically, BCIE can be divided into two groups: BCIE with or without palmoplantar involvement, associated with mutations in keratin 1 or keratin 10, respectively. Here we report a newborn with generalized erythema, blistering and erosions at the time of birth. No hyperkeratosis was seen on the palms and soles. The lack of palmoplantar involvement suggested that keratin 10 could be involved. DNA analysis showed a known mutation in the keratin 10 gene.
Ossification of the auricle
Authors: Sterneberg‐Vos Heleen , Winnepenninckx Veronique , Frank Jorge , Kelleners‐Smeets Nicole W. 
Published: October 2007

AbstractThe auricle is a flexible structure because it is formed by elastic cartilage. In the rare occurrence of ectopic auricular ossification, however, the cartilage is replaced by lamellar bone and becomes petrified. Such unphysiological replacement has been reported in association with local trauma, inflammation, systemic diseases, and hypothermia. Here, we report a patient with unilateral ossification of the auricle secondary to frostbite.
Epidermodysplasia verruciformis: unsuccessful therapeutic approach with imiquimod
Authors: Janssen Kathleen , Lucker Georges P. H. , Houwing Ronald H. , Van Rijssel R. 
Published: October 2007

AbstractEpidermodysplasia verruciformis (EV) is a rare heritable disease characterized by an unusual susceptibility to infection with specific types of human papillomavirus and a propensity for developing skin malignant tumors. Partial defects in cell‐mediated immunity have been demonstrated in this disease. Here, we describe a patient with EV who was treated unsuccessfully with imiquimod.
Porphyria cutanea tarda in pre‐existent lupus erythematosus – is there an association?
Authors: Van Tuyll van Serooskerken Anne‐Moon , Habets J. M. Werner , Badeloe Sadhanna , Poblete‐Gutiérrez Pamela , Frank Jorge 
Published: October 2007

AbstractIn lupus erythematosus (LE), vesicles and bullae are only rarely seen. However, in some instances such efflorescences might suggest an association with distinct cutaneous diseases, including erythema multiforme, toxic epidermal necrolysis or autoimmune blistering disorders such as bullous pemphigoid, pemphigus vulgaris, and dermatitis herpetiformis Duhring. Another blistering disease that has been described in association with cutaneous and systemic LE is porphyria cutanea tarda (PCT). PCT is a metabolic disorder caused by a deficiency of the fifth enzyme in heme biosynthesis, uroporphyrinogen decarboxylase. Here, we report on a 57‐year‐old Caucasian woman of Dutch origin with a medical history of mild cutaneous LE who developed skin fragility, blistering skin lesions, milia, and facial hypertrichosis. Subsequent porphyrin analysis in urine and feces confirmed the suspected simultaneous manifestation of LE and PCT.
Haplotype‐specific pattern of association of human major histocompatibility complex with non‐Hodgkin’s lymphoma outcome
Authors: Nowak J. , Kalinka‐Warzocha E. , Juszczyński P. , Mika‐Witkowska R. , Zajko M. , Graczyk‐Pol E. , Coiffier B. , Salles G. , Warzocha K. 
Published: October 2007

AbstractIn the previous studies, some human major histocompatibility complex (MHC) genes such as TNF, LTA and human leukocyte antigen (HLA)‐DR2 genes and A1‐B8‐TNF−308A haplotype were implied in non‐Hodgkin’s lymphoma (NHL) outcome. In the current study, we have assigned most probable six‐locus haplotypes determined by HLA‐A, ‐Cw, ‐B and ‐DRB1 highly polymorphic genes and non‐HLA LTA+252 and TNF−308 single nucleotide polymorphisms (SNPs) in 152 NHL Caucasian French patients. We have broadly mapped the MHC region by its component blocks and tagging alleles. Ten frequent (with haplotype frequency >1%) six‐locus extended haplotypes (EHs) were revealed in NHL patients. The only two adjacent locus fragment of 8.1 EH associated with shortened freedom from progression (FFP) was B*08‐LTA+252G (P= 0.0084, RR = 2.45). Interestingly, 305‐kbp‐long, four‐locus fragment of 8.1 EH, Cw*07‐B*08‐LTA+252G‐TNF−308A block was much strongly associated with shortened FFP (P= 0.00045, RR = 3.26). The analysis of further extended haploblocks comprising five or six loci showed weaker association with outcome measures, suggesting linkage disequilibrium to be the cause of DRB1*03 and A*01 allele associations. In contrast, all fragments of 7.1 EH influenced FFP favorably with top association of TNF−308G allele. In multivariate analysis, only Cw*07‐B*08‐LTA+252G‐TNF−308A and TNF−308G‐DRB1*01 haplotypes remained predictive for shortened FFP (P= 0.024 and 0.027, respectively) and independent of International Prognostic Index (P= 0.00044). This study reveals that the block composition of EHs may cause important functional differences for NHL outcomes. Further study will be required in NHL patients by fine mapping with dense microsatellite or SNP tags to define susceptibility genes in associating regions.
Prolonged Gestation in Two Holstein Cows: Transabdominal Ultrasonographic Findings in Late Pregnancy and Pathologic Findings in the Fetuses
Authors: Buczinski S. , Bélanger A.‐M. , Fecteau G. , Roy J.‐P. 
Published: October 2007

SummaryProlonged pregnancy in cattle is a rare condition that is commonly accompanied with fetal adenohypophyseal hypoplasia. The final diagnosis is often challenging as breeding date error remains possible in virtually all situations. In this report, fetal wellbeing assessment is depicted in two Holstein cows suffered from prolonged pregnancy. Transabdominal ultrasonographic findings were compatible with healthy non‐stressed fetuses. The clinical and pathologic findings encountered in both fetuses expelled after corticosteroid induction of parturition consisted of adenohypophyseal aplasia and hydronephrosis. Other nervous system anomalies (hydrocephaly or holocephaly) were observed. Tetralogy of Fallot was also found in one calf.
Pharmacokinetics and Mammary Residual Depletion of Erythromycin in Healthy Lactating Ewes
Authors: Goudah A. , Sher Shah S. , Shin H.C. , Shim J.H. , Abd El‐Aty A. M. 
Published: October 2007

SummaryThe aim of this investigation was to examine the pharmacokinetics and mammary excretion of erythromycin administered to lactating ewes (n = 6) by the intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) routes at a dosage of 10 mg/kg. Blood and milk samples were collected at pre‐determined times, and a microbiological assay method was used to measure erythromycin concentrations in serum and milk. The concentration–time data were analysed by compartmental and non‐compartmental kinetic methods. The serum concentration–time data of erythromycin were fit to a two‐compartment model after i.v. administration and a one‐compartment model with first‐order absorption after i.m. and s.c. administration. The elimination half‐life (t1/2β) was 4.502 ± 1.487 h after i.v. administration, 4.874 ± 0.296 h after i.m. administration and 6.536 ± 0.151 h after s.c. administration. The clearance value (Cltot) after i.v. dosing was 1.292 ± 0.121 l/h/kg. After i.m. and s.c. administration, observed peak erthyromycin concentrations (Cmax) of 0.918 ± 0.092 μg/ml and 0.787 ± 0.010 μg/ml were achieved at 0.75 and 1.0 h (Tmax) respectively. The bioavailability obtained after i.m. and s.c. administration was 91.178 ± 10.232% and 104.573 ± 9.028% respectively. Erythromycin penetration from blood to milk was quick for all the routes of administration, and the high AUCmilk/AUCserum (1.186, 1.057 and 1.108) and Cmax‐milk/Cmax‐serum ratios reached following i.v., i.m. and s.c. administration, respectively, indicated an extensive penetration of erythromycin into the milk.
Intra‐tumoral Gene Delivery of feIL‐2, feIFN‐γ and feGM‐CSF using Magnetofection as a Neoadjuvant Treatment Option for Feline Fibrosarcomas: A Phase‐I Study
Authors: Jahnke A. , Hirschberger J. , Fischer C. , Brill T. , Köstlin R. , Plank C. , Küchenhoff H. , Krieger S. , Kamenica K. , Schillinger U. 
Published: October 2007

SummaryDespite aggressive pre‐ or postoperative treatment, feline fibrosarcomas have a high relapse rate. In this study, a new treatment option based on immune stimulation by intra‐tumoral delivery of three feline cytokine genes was performed. The objective of this phase‐I dose‐escalation study was to determine a safe dose for further evaluation in a subsequent phase‐II trial. Twenty‐five client‐owned cats with clinical diagnosis of fibrosarcoma – primary tumours as well as recurrences – entered the study. Four increasing doses of plasmids coding for feIL‐2, feIFN‐γ or feGM‐CSF, respectively, were previously defined. In groups I, II, III and IV these doses were 15, 50, 150 and 450 μg per plasmid and a corresponding amount of magnetic nanoparticles. Two preoperative intra‐tumoral injections of the magnetic DNA solution were followed by magnetofection. A group of four control cats received only surgical treatment. Side effects were registered and graded according to the VCOG‐CTCAE scale and correlated to treatment. Statistical analyses included one‐wayanova,post hocand Kruskal–Wallis tests. ELISA tests detecting plasma feIFN‐γ and plasma feGM‐CSF were performed. One cat out of group IV (450 μg per plasmid) showed adverse events probably related to gene delivery. As these side effects were self‐limiting and occurred only in one of eight cats in group IV, this dose was determined to be well tolerable. Altogether six cats developed local recurrences during a 1‐year observation period. Four of these cats had been treated with dose IV. Regarding these observations, a subsequent phase‐II trial including a representative amount of cats should be tested for the efficacy of dose IV as well as dose III.
Rural and remote health research: Key issues for health providers in southern Queensland
Authors: Eley Rob M. , Baker Peter G. 
Published: October 2007

AbstractObjective:  To determine what community health service providers in rural southern Queensland considered were major issues affecting their efficacy. Results will inform the future research strategy of the Centre for Rural and Remote Area Health with the aim of addressing specific regional needs. Design:  Interactive research workshops. Setting:  Health providers and other key stakeholders. Subjects:  Participants from organisations directly involved with health care were complemented by representatives from local government, the police service and church groups. Main outcome measures:  The workshops used the nominal group technique to identify what participants considered were key health issues in their locations. These issues were then prioritised by the participants. Thematic analysis of the issues generated a ranking of themes by importance. Results were compared with a similar exercise undertaken in 2003. Results:  Seventeen themes were identified, with workforce by far the major concern of health providers. Recruitment and retention of health workers were the principal issues of concern. The other four highest ranked themes across all workshops were mental health care, access to health services, perceptions and expectations of consumers, and interagency cooperation. The workshops provided important information to the Centre for Rural and Remote Area Health for developing research strategy. Additionally, new alliances among health providers were developed which will support sharing of information and resources.Conclusion:  The workshops enabled organisations to meet and identify the key health issues and supported research planning. New alliances among health providers were forged, and collaborative research avenues are being explored. The workshop forum is an excellent means of information exchange.
Evaluating the effectiveness of a university health sciences experience program for regional Year 10 students
Authors: Penman Joy , Ellis Bronwyn , White Frances , Misan Gary 
Published: October 2007

AbstractObjective:  The main aim of this pilot program was to raise secondary students' awareness of university studies and careers in the health sciences through a three‐day program. Further aims of the program were to increase the participants' knowledge of health issues through group activities, and provide an opportunity to interact with university staff and health professionals. Design:  The participants were surveyed at the conclusion of the program using an online questionnaire (30 items) consisting of both open and closed questions. Setting:  The program and evaluation were carried out at a regional campus of a metropolitan‐based university during the mid‐year vacation. Participants:  Fourteen Year 10 students (11 female, 3 male; 5 from government schools, 9 from a non‐government school) from a regional city completed the evaluation. Main outcome measures:  Satisfaction with the program, increased awareness of pathways into university, and changes in attitude about university as a career option. Results:  The evaluation revealed that the participants had had a pleasant learning experience, which had led them to feel positive about university studies in general and as an option for themselves. They made useful suggestions for improvements. Conclusion:  The evaluation of the effectiveness of the program showed that it had achieved its objectives. It also provided the program organisers with insights regarding timing and activities, which will be useful in improving future programs targeting secondary students.
Retrograde axonal transport obstruction of brain‐derived neurotrophic factor (BDNF) and its TrkB receptor in the retina and optic nerve of American Cocker Spaniel dogs with spontaneous glaucoma
Authors: Iwabe Simone , Moreno‐Mendoza Norma A. , Trigo‐Tavera Francisco , Crowder Cheryl , García‐Sánchez Gustavo A. 
Published: October 2007

AbstractObjective To determine the degree of retrograde optic nerve axonal transport obstruction at the scleral lamina cribosa level by examining levels of brain‐derived neurotrophic factor (BDNF) and its tyrosine kinase receptor type B (TrkB) in dogs presenting with high intraocular pressure.Animals studied A total of 10 eyes, four normal and six glaucomatous eyes, from normal and affected American Cocker Spaniels with primary glaucoma were studied. All eyes were assessed by neuro‐ophthalmic examination, tonometry, gonioscopy, slit‐lamp biomicroscopy, and indirect ophthalmoscopy prior to enucleation.Methods Immunocytochemistry analysis was performed to evaluate BDNF and TrkB receptor expression in retina and optic nerve in normal and glaucomatous dogs.Results In all normal eyes BDNF immunostaining was detected in the cytoplasm of retinal ganglion cells (RGC), inner plexiform layer (IPL), inner nuclear layer (INL), nerve fiber layer (NFL), optic nerve head cells, and lamina cribosa cells. In all glaucomatous eyes BDNF was more evident in RGC, NFL and lamina cribosa cells. TrkB receptor was detected in the cytoplasm of RGC, NFL and ONH bundles in all normal eyes, and in a more intense pattern in all glaucomatous eyes.Conclusions BDNF retrograde axonal transport is substantially inhibited by intraocular pressure elevation. TrkB accumulation at the ONH in glaucoma suggests a role for neurotrophin deprivation in the pathogenesis of RGC death in canine glaucoma, as well as a possible paracrine and/or autocrine signaling within the lamina cribosa. Neurotrophin signaling may regulate more than neuronal development, survival and differentiation. BDNF neurotrophin and its TrkB receptor expression by lamina cribosa cells and ONH astrocytes in glaucomatous eyes may help to determine the role of these cells as a paracrine source in terms of retinal ganglion cell survival, during episodes of elevated intraocular pressure.
Blood flow velocity response of the ophthalmic artery and anterior optic nerve head capillaries to carbogen gas in the rhesus monkey model of optic nerve head ischemia
Authors: Brooks Dennis E. , Komaromy Andras M. , Kallberg Maria E. , Miyabashi Taka , Ollivier Franck J. , Lambrou George N. 
Published: October 2007

AbstractPurpose  To determine the effect on blood flow velocity of the ophthalmic artery and anterior superficial optic nerve head (ONH) capillaries by changing inhaled gas from 100% oxygen to carbogen (95% oxygen, 5% CO2) in rhesus monkeys receiving chronic unilateral orbital endothelin‐1 administration.Methods  The right eye of six young male rhesus monkeys (Macaca mulatta) received endothelin‐1 (ET‐1) by osmotic minipumps to the perineural optic nerve (0.3 µg/day) for 8 months. Three additional monkeys (control group) received the ET‐1 vehicle (Sham) solution to the right optic nerve for the same period of time. The left eye served as a nontreated control in both groups. The blood flow velocities of the anterior ONH capillaries and ophthalmic artery were assessed in both eyes using confocal laser scanning flowmetry (CSLF) and color Doppler imaging (CDI), respectively.Results  A slight increase in the CDI blood flow velocities and a small decrease in the resistive index of the ophthalmic artery, and increased flow of the ONH capillaries in rhesus monkeys were detected when inhaled gas was changed from 100% oxygen to carbogen. The difference in CSLF blood flow in the nasal ONH between the endothelin‐1 (ET‐1) treated right eye and the normal left eye of the same individual monkeys was significantly greater than the difference in blood flow between the Sham‐treated right eye and the normal left eye in control animals under the conditions of carbogen and oxygen inhalation.Conclusion  Carbogen inhalation slightly influences the microcirculation of the globe and ONH in rhesus monkeys. These data suggest that low dose ET‐1 administration has a subtle vasorelaxing effect in the ONH microcirculation in this animal model of ONH ischemia.
Immunolocalization of myocilin protein in the anterior eye of normal and primary open‐angle glaucomatous dogs
Authors: Hart H. , Samuelson D. A. , Tajwar H. , MacKay E. O. , Lewis P. A. , Kallberg M. , Gelatt K. N. 
Published: October 2007

AbstractObjective  The presence of myocilin was investigated in a colony of Beagles, a canine model for inherited primary open‐angle glaucoma (POAG). The myocilin protein was localized in the normal and glaucomatous canine eyes by immunohistochemistry and immunocytochemistry.Methods  Paraffin‐ and plastic‐embedded specimens from the anterior uveas of 10 Beagles with inherited glaucoma (3 months to 13 years old) and 6 age‐matched normal dogs were sectioned, and were then incubated with primary antibody, rabbit polyclonal antihuman MYOC IgG, overnight at 4 °C. Specimens were incubated with secondary antibody with one of the following: biotinylated link followed by peroxidase‐labeled streptavidin and then by substrate‐chromogen for light microscopy; fluorescent marker Texas red; or 18 nm colloidal gold‐labeled goat antirabbit IgG for transmission electron microscopy.Results  With normal, pre‐ and early glaucomatous canine specimens, cell membranes of smooth muscle cells of the iris and ciliary body stained positively, as well as most resident stromal and vascular endothelial cells. The cytoplasm of cells within the nonpigmented ciliary epithelium of the ciliary body processes stained intensely, being weaker along the pars plana. Trabecular meshwork (TM) cells and surrounding extracellular matrix labeled, as well as the sclera adjacent to the angular aqueous plexus. In specimens with moderate and advanced glaucoma, greater intensity of staining was observed within TM cells and adjacent sclera, and portions of the nonpigmented epithelium of the ciliary processes. Fibrinous material labeled intensely within the posterior chamber.Conclusions  Myocilin in the normal and glaucomatous canine eye was successfully immunolocalized. These findings with regard to the normal eye are nearly identical to those previously reported in humans, and support the original hypothesis that there is an increase in both accumulation and localization of myocilin in glaucomatous canine eyes. It also supports the possibility that changes in the activity of myocilin within the aqueous humor outflow pathway of individuals with spontaneous glaucoma are associated with the rise of intraocular pressure and subsequent development of this disease, but may not be the primary event in the initial raise in intraocular pressure in POAG in the Beagle.
Ocular melanosis in the Cairn Terrier: clinical description and investigation of mode of inheritance
Authors: Petersen‐Jones Simon M. , Forcier Janice , Mentzer A. Lexi 
Published: October 2007

AbstractObjective  To describe the clinical features of ocular melanosis in Cairn Terriers.Animal studied  One hundred and fourteen Cairn Terriers diagnosed with ocular melanosis.Procedure(s)  A complete eye examination was performed on each dog. Four dogs (and two unaffected control dogs) underwent a high frequency ultrasound examination of the anterior segment. The pedigrees of affected dogs were analyzed.Results  Forty‐four (38.6%) dogs were male and 67 (58.7%) female; the sex of three dogs (2.6%) was not provided. A four‐stage grading system of the ocular changes was developed. There was a variable age of onset, and the earliest change was a dark‐colored thickening of the iris root. This was followed by the development of episcleral/scleral pigment plaques, release of pigment into the aqueous and deposition in the drainage apparatus, particularly ventrally. Secondary glaucoma developed in the most severely affected dogs. A slow progression of pigmentation in the tapetal fundus was observed and in some dogs pigment on the surface of the optic nerve head was seen. Three dogs developed uveal melanocytic neoplasms. Pedigree analysis suggested a possible autosomal dominant mode of inheritance.Conclusions  Ocular melanosis is an inherited, probably autosomal‐dominant condition with a variable age of onset and rate of progression. It results in a thickening and pigmentation of the iris, release of pigmented material into the aqueous, pigment deposition in the sclera/episclera, and to a lesser extent posterior segment pigment deposition. Following extensive pigment deposition in the aqueous drainage pathways it can result in secondary glaucoma.
Primary intraocular primitive neuroectodermal tumor (retinoblastoma) causing unilateral blindness in a gelding
Authors: Knottenbelt Derek C. , Hetzel Udo , Roberts Veronica 
Published: October 2007

AbstractA 14‐year‐old gray gelding was presented for investigation of a visible, pale‐colored ocular mass in the right eye. An intraocular mass was identified clinically and ultrasonographically as originating from the superior nasal quadrant of the ciliary body and retina. The mass occupied the majority of the vitreous chamber and some of the superior anterior chamber of the eye. The affected eye was blind. Following exenteration, a primary intraocular primitive neuroectodermal tumor (i.e. a retinoblastoma/medulloepithelioma), a rarely described intraocular mass in adult horses, was identified by pathologic examination. The gelding returned to normal use following a short recovery period.