bluuebunny/crossref_metadata_2025_split huggingface dataset:
Papillary carcinoma with nodular fasciitis‐like stroma—A case report in pregnancy
Authors: Leal Isabel Irene Rama , Carneiro Fabiana Pirani , Basílio‐de‐Oliveira Carlos Alberto , Magalhães Albino Verçosa , Barbosa Tânia Wanderley Paes , da Silva Francileide Paes , de Castro Montenegro Frederico Sérgio Luis 
Published: January 2008

AbstractWe report a case of Papillary carcinoma with nodular fasciitis‐like stroma that is a rare variant of Papillary carcinoma characterized by a prominent stromal cell proliferation that causes difficulties in cytologic and histologic diagnosis. The patient was a 34‐year‐old woman, pregnant, presented with a 1‐year history of a growing mass in neck, dysphagia, and hoarseness. Physical examination revealed a firm nodular mass in thyroid gland. The fine needle aspiration biopsy specimen contained, besides diagnostic epithelial features of Papillary thyroid carcinoma, discohesive arrangement of bland spindle cells. Macroscopically, the specimen consisted of nodular tumor measuring 10 × 6 × 6 cm. Histologically the tumor was composed of small foci of neoplastic epithelial component distributed in abundant stroma. In immunohistochemistry, spindle cells in the stroma were positive for α‐smooth muscle actin and the neoplastic cells showed positive staining for TTF‐1 and progesterone receptor. Diagn. Cytopathol. 2008;36:139–141. © 2008 Wiley‐Liss, Inc.
The clinical and diagnostic impact of using standard criteria of adequacy assessment and diagnostic terminology on thyroid nodule fine needle aspiration
Authors: Jing Xin , Michael Claire W. , Pu Robert T. 
Published: January 2008

AbstractThe study was aimed to investigate the impact of using standard criteria for assessing specimen adequacy and diagnostic terminology (CAST) on fine‐needle aspiration (FNA) diagnosis and clinical management of thyroid nodules. The study included similar numbers of FNAs performed in 2 year before (group A) and 1.5 year after (group B) implementing the standard CAST. In comparison to group A, group B showed a significantly lower rate of nondiagnostic specimens (RND) (16.1% vs. 21.6%, P ≤ 0.01) and rate of descriptive diagnoses (RDD) (3.8% vs. 14.5%, P ≤ 0.001) and greater non‐neoplastic (70.0% vs. 64.1%, P < 0.05) and follicular cell lesions (7.4% vs. 4.3%, P < 0.05) but a similar percentage of neoplastic diagnoses. The rate of surgical follow‐up (RSF) was significantly higher in group B than in group A, overall (21.6% vs. 17.0%, P < 0.05), or in subgroups of non‐neoplastic (12.6% vs. 5.4%, P < 0.01) and neoplastic categories (81.0% vs. 61.0%, P < 0.05). The rate of cytohistologic concordance was higher in group B although the difference was not statistically significant. We concluded that use of the standard CAST on FNA diagnosis of thyroid nodules significantly reduced RND and RDD, providing more consistent diagnoses among the pathologists as well as better and more uniform communication between the pathologists and the clinicians. Furthermore, the cytohistological concordance was slightly better after CAST implementation, indicating that the improvement of diagnostic consistency among pathologists did not sacrifice the diagnostic accuracy. Diagn. Cytopathol. 2008;36:161–166. © 2008 Wiley‐Liss, Inc.
Comparison of p16INK4A and Hybrid Capture® 2 human papillomavirus testing as adjunctive tests in liquid‐based gynecologic SurePath™ preparations
Authors: Nassar Aziza , O'Reilly Katie , Cohen Cynthia , Siddiqui Momin T. 
Published: January 2008

Abstractp16INK4a, cyclin‐dependent kinase inhibitor, is functionally inactivated in many tumors, including cervical cancer. We compared p16INK4A immunocytochemical staining and Hybrid Capture® 2 (HCII) on SurePath™ specimens using tissue biopsies (as the gold standard). Their utility in a spectrum of atypical and preneoplastic lesions, and their ability to accurately identify underlying lesions of CIN II or greater was assessed using biopsy follow‐up data. One‐hundred and seventeen residual SurePath™ samples were collected: 43 atypical squamous cells of undetermined significance (ASCUS), 47 low‐grade (LGSIL), and 27 high‐grade (HGSIL) squamous intraepithelial lesions. Two slides were prepared from each sample; one stained with the SurePath™ autocyte stain and one immunostained using the CINtec™ p16INK4a Cytology Kit (Dakocytomation). High‐risk HPV testing was performed using the HCII DNA test (Digene, Gaithersburg, MD). Available tissue biopsy follow‐up data was retrieved. p16INK4a was positive in 32.6% (14/43) ASCUS, 46.8% (22/47) LGSIL, and 48.1% (13/27) HGSIL specimens. HCII DNA test was positive in 41.9% (18/43) ASCUS, 78.7% (37/47) LGSIL, and 96.3% (26/27) HGSIL samples. The sensitivity, specificity, positive (PPV) and negative (NPV) predictive values of p16INK4a and HCII were: 58.7% and 89.8%, 58.6% and 34.6%, 69.2% and 72.1%, 47.2% and 64.3%, respectively. In patients with cervical biopsies, the PPV of HCII (92.3%) results for a biopsy with CINII/III was significantly higher than the PPV of p16INK4a (52%) (P = 0.001). Using liquid‐based cytology specimens, HCII is a more sensitive test than p16INK4a for detection of abnormal cytology. HCII has a higher PPV than p16INK4a for identifying CIN II/III. Diagn. Cytopathol. 2008;36:142–148. © 2008 Wiley‐Liss, Inc.
Factors Influencing Anion Binding Stoichiometry: The Subtle Influence of Electronic Effects
Authors: Lowe Adam J. , Dyson Gail A. , Pfeffer Frederick M. 
Published: January 2008

AbstractSix new, charge‐neutral norbornene‐based receptors 1a,1b–3a,3b were prepared, and their ability to interact with simple anions in DMSO was investigated using 1H NMR and UV/Vis spectroscopy. Binding of dihydrogenphosphate by the six receptors appeared to be based solely on steric constraints. In contrast, the binding stoichiometry of 3a and 3b to acetate was controlled by subtle electronic factors.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Palladium‐Catalyzed Silylation of Electron‐Deficient Aryl Iodides Using Triorganosilane in the Presence of Pyridine and LiCl
Authors: Iizuka Muneaki , Kondo Yoshinori 
Published: January 2008

AbstractPalladium‐catalysed silylation of aryl iodides with electron‐withdrawing groups was efficiently achieved using pyridine and lithium chloride as additives and conducting the reaction at room temperature. Functionalized aryl[2‐(hydroxymethyl)phenyl]dimethylsilanes were also prepared by palladium‐catalysed reaction using THP‐protected [2‐(hydroxymethyl)phenyl]dimethylsilane as a silylating agent followed by deprotection. Rhodium‐catalysed 1,4‐addition of the arylsilane was carried out using cyclohexenone as an enone in excellent yield. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Strength vs. Accessibility: Unraveling the Patterns of Self‐Recognition in a Conformationally Locked Amino Alcohol (Eur. J. Org. Chem. 5/2008)
Authors: Mehta Goverdhan , Sen Saikat , Guru Row Tayur N. , Chopra Deepak , Chattopadhyay Sayani 
Published: January 2008

AbstractThe cover picture shows the iso‐surface of a novel amino alcohol molecule. The packing in the crystalline phase is determined principally by O?H···N hydrogen bonds and rarely encountered weak π‐π stacking interactions between the isolated double bonds. The acidic, but less accessible hydrogen atoms of the tertiary NH2 group in the amino alcohol remain as mere spectators in the supramolecular assembly, which therefore breaks a well‐known crystallographic lore. Details are discussed in the article by G. Mehta et al. on p. 805 ff.
Extracting stoichiometry, thermodynamics, and kinetics for the interaction of DNA with cadmium ion by capillary electrophoresis on‐line coupled with electrothermal atomic absorption spectrometry
Authors: Li Yan , Xia Yun‐Long , Jiang Yan , Yan Xiu‐Ping 
Published: January 2008

AbstractUnderstanding the binding of cadmium with DNA is of great importance for elucidating the mechanism of cadmium genotoxicity and carcinogenicity. In the present work, CE on‐line coupled with electrothermal atomic absorption spectrometry was employed to study the binding electrophoretic behaviors, stoichiometry, thermodynamics, and kinetics for the interaction of cadmium cation (Cd(II)) with DNA. The stoichiometry (Cd(II) to DNA (as the concentration of base pairs)) for the interaction was determined to be 1:5. Two types of binding sites on DNA were observed with the binding constants of 106 and 105 L/mol, respectively, showing strong affinity of Cd(II) to DNA. The interaction of Cd(II) with both types of binding sites on DNA were driven by negative enthalpy change with a large positive entropy change. The binding of Cd(II) to DNA followed a first‐order kinetics for Cd(II) with the apparent activation energy of 45.7 ± 1.9 kJ/mol. The results obtained in present investigation would be helpful to understanding the genotoxicity and carcinogenicity of cadmium.
Heparin inhibits osteoclastic differentiation and function
Authors: Ariyoshi Wataru , Takahashi Tetsu , Kanno Takahiro , Ichimiya Hisashi , Shinmyouzu Kouhei , Takano Hiroshi , Koseki Takeyoshi , Nishihara Tatsuji 
Published: January 2008

AbstractWe investigated the effects of Glycosaminoglycans (GAGs) on mouse monocytic cell line in regard to their differentiation, proliferation, and function in vitro. RAW 264.7 cells were cultured with receptor activator of NF‐κB ligand (RANKL) and various GAGs. Osteoclastic cells were visualized by staining for tartrate‐resistant acid phosphatase (TRAP) and detected using a phenyl‐phosphate substrate method. RAW 264.7 cells were also cultured with stimulants contained in BD BioCoat OSTEOLOGICTM kit, and bone resorption activity was assessed by counting the numbers of resorption pits. We also examined the effect of heparin on cell growth using MTT assay, while the expression level of c‐Src protein was determined by immunoblot analysis. Heparin suppressed TRAP‐positive multinucleated cell formation and TRAP activity induced by RANKL, whereas the other GAGs showed no effects on osteoclast differentiation. Heparin also inhibited the formation of resorption pits, while the others did not. In the MTT assay, none of the tested GAGs had an influence on RAW 264.7 cell proliferation. However, heparin reduced the level of c‐Src protein in RAW 264.7 cells stimulated with RANKL. To determine the affinity of heparin and RANKL, they were subjected by HiTrap heparin column chromatography and each fraction was collected. Western blotting analysis revealed the expression of RANKL in the fraction bound to heparin. The binding of RANKL and heparin was confirmed by quartz‐crystal microbalance. These results indicate that the inhibitory effect of heparin toward osteoclastogenesis induced by RANKL is due to the binding of heparin to RANKL. J. Cell. Biochem. 103: 1707–1717, 2008. © 2008 Wiley‐Liss, Inc.
Phosphate and amide III mapping in sialoliths with Raman microspectroscopy
Authors: Kinoshita H. , Miyoshi N. , Miyoshi K. , Ogawa T. , Ogasawara T. , Kitagawa Y. , Itoh H. , Sano K. 
Published: January 2008

AbstractSialoliths, a cause of the salivary gland infection, are reported to be composed of inorganic and organic substances. However, the precise mechanism of sialolith formation remains unclear. The purpose of this report is to elucidate this mechanism by analyzing the precise distribution of phosphate (an inorganic substance) and amide III (an organic substance) in sialoliths by using Raman microspectroscopy. Sialoliths from the submandibular gland duct were analyzed by this form of observation and by a scanning electron microscope (SEM) equipped with an energy‐dispersive X‐ray spectroscope (EDX). In Raman microspectroscopy we analyzed the spectral peak of the phosphate (PO43−) symmetric stretching vibrational mode (υ1) at 960 cm−1 and that of amide III at 1265 cm−1 to demonstrate the mapping of an image of these elements showing a semiquantitative distribution of phosphate and amide III in the sialoliths. It was found that phosphate and amide III were concentrated at the center of the sialoliths, and the phosphate distribution in the sialoliths showed concentric laminations. These results indicated the possibility that the sialoliths originated from a nidus of organic materials and progressively grew by the deposition of layers of organic and inorganic materials. Copyright © 2008 John Wiley & Sons, Ltd.
Raman spectroscopy study of the Fermi resonance between a local vibration and the two‐phonon spectrum in PbI2 crystal
Authors: Yaremko A. , Dorogan V. , Motsnyi F. 
Published: January 2008

AbstractThe problem of complex Fermi resonance (FR) involving a local vibration is considered. It is shown for the first time that in the PbI2 crystal, owing to the features of phonon bands, the following two effects superpose on each other: (1) the traditional FR between the fundamental Ω(A1g) vibration and the two‐phonon states (TPS) arising from the 2Ω(Eu) vibrations, and (2) the FR between the impurity vibration and the same TPS band. It is shown theoretically that in the case of such complex FR the shape of the spectrum strongly depends on the impurity concentration, anharmonic constants and the phonon band structure. An analysis of experimental data and comparison with theory is made. Copyright © 2008 John Wiley & Sons, Ltd.
Experimental and statistical analysis methods for peptide detection using surface‐enhanced Raman spectroscopy
Authors: Mitchell Breeana L. , Patwardhan Anil J. , Ngola Sarah M. , Chan Selena , Sundararajan Narayan 
Published: January 2008

AbstractSurface‐enhanced Raman spectroscopy (SERS) has the potential to make a significant impact in biology research due to its ability to provide information orthogonal to that obtained by traditional techniques such as mass spectrometry (MS). While SERS has been well studied for its use in chemical applications, detailed investigations with biological molecules are less common. In addition, a clear understanding of how methodology and molecular characteristics impact the intensity, the number of peaks, and the signal‐to‐noise of SERS spectra is largely missing. By varying the concentration and order of addition of the SERS‐enhancer salt (LiCl) with colloidal silver, we were able to evaluate the impact of these variables on peptide spectra using a quantitative measure of spectra quality based on the number of peaks and peak intensity. The LiCl concentration and order of addition that produced the best SERS spectra were applied to a panel of synthetic peptides with a range of charges and isoelectric points (pIs) where the pI was directly correlated with higher spectral quality. Those peptides with moderate to high pIs and spectra quality scores were differentiated from each other using the improved method and a hierarchical clustering algorithm. In addition, the same method and algorithm was applied to a set of highly similar phosphorylated peptides, and it was possible to successfully classify the majority of peptides on the basis of species‐specific peak differences. Copyright © 2008 John Wiley & Sons, Ltd.
Micro‐Raman spectroscopy of pigments contained in different calcium carbonate polymorphs from freshwater cultured pearls
Authors: Soldati A. L. , Jacob D. E. , Wehrmeister U. , Häger T. , Hofmeister W. 
Published: January 2008

AbstractThe metastable calcium carbonate polymorph vaterite has recently been found to occur commonly in freshwater cultured pearls from Japan and China. Organic pigment molecules in these vaterite regions of freshwater cultured pearls were analysed by resonance and near‐resonance micro‐Raman spectroscopy, in single spectra and in mapping modes and are compared with their aragonitic counterparts. Four different excitation wavelengths (487.9, 514.5, 532.2 and 632.8 nm) were used, resulting in differential increase of resolution for the pigments. Although vaterite areas were always lightly coloured (light yellow, light brown or white), ten different pigments were identified and a single colour is formed by a combination of three and up to nine polyenes of different lengths. The molecules were identified as unmethylated polyenes with effective chain lengths between 8 and 12 conjugated double bonds and FWHMs of 10 ± 2 cm−1. Additionally, and for the first time in pearls, methylated polyenes were detected together with unmethylated pigments in aragonite. Polyenes with chain lengths shorter than 8 or longer than 12 were not found in vaterite, whereas both shorter and longer pigment chains were observed in aragonite from the same pearls. Pigments have lower concentrations in vaterite and are distributed more homogeneously, while they have higher concentrations in aragonite and are arranged along the annual growth lines of the pearls. Copyright © 2008 John Wiley & Sons, Ltd.
Raman spectroscopic study of the uranyl carbonate mineral zellerite
Authors: Frost Ray L. , Dickfos Marilla J. , Čejka Jiří 
Published: January 2008

AbstractRaman and infrared spectra of the uranyl mineral zellerite, Ca[(UO2)(CO3)2(H2O)2]·3H2O, were measured and tentatively interpreted. UO bond in uranyl and OH···O hydrogen bonds were calculated from the vibrational spectra. The presence of structurally nonequivalent water molecules in the crystal structure of zellerite was inferred. A proposed chemical formula of zellerite is supported. Raman bands at 3514, 3375 and 2945 cm−1and broad infrared bands at 3513, 3396 and 3326 cm−1 are related to the ν OH stretching vibrations of hydrogen‐bonded water molecules. Observed wavenumbers of these vibrations prove that in fact hydrogen bonds participate in the crystal structure of zellerite. The presence of two bands at 1618 and 1681 cm−1 proves structurally distinct and nonequivalent water molecules in the crystal structure of zellerite. Copyright © 2008 John Wiley & Sons, Ltd.
Bichromophoric behavior of nitrophenyl‐triazene anions: a resonance Raman spectroscopy investigation
Authors: Ando Rômulo A. , dos Santos Ana P. V. , Hörner Manfredo , Santos Paulo S. 
Published: January 2008

AbstractHighly delocalized molecular frameworks with intense charge transfer transitions, known as push‐pull systems, are of central interest in many areas of chemistry, as is the case of nitrophenyl‐triazene derivatives. The 1,3‐bis(2‐nitrophenyl)triazene and 1,3‐bis(4‐nitrophenyl)triazene were investigated by electronic (UV‐Vis) and resonance Raman (RR) spectroscopies. The bichromophoric behavior of 1,3‐bis(4‐nitrophenyl)triazene anion opens the possibility of tuning with visible radiation, two distinct electronic states. The RR profiles of nitrophenyl‐triazene derivatives clearly show that the first allowed electronic state can be assigned to a charge transfer from the ring π system to the NO2moiety (ca520 nm), while the second, as a charge transfer from N3−to the aromatic ring (ca390 nm). In thepara‐substituted derivative, a more efficient electron transfer and a greater energy separation between the two excited states are observed. Copyright © 2008 John Wiley & Sons, Ltd.
Characterisation and functional properties of proteins of some Indian chickpea (Cicer arietinum) cultivars
Authors: Deep Singh Gagan , Wani Ali Abas , Kaur Devinder , Sogi Dalbir Singh 
Published: January 2008

AbstractBACKGROUND: Chickpea (Cicer arietinum L.) proteins have received attention during recent years owing to their higher biological values and better functional ingredients than oilseed proteins. In this study the composition, fractionation, electrophoretic behaviour and functional properties of five chickpea protein concentrates were determined.RESULTS: The chickpea proteins contained 15.9–54.8 g kg−1 albumin, 48.9–154.1 g kg−1 globulin, 39.2–76.5 g kg−1 glutelin and traces of prolamin. Electrophoresis of the various fractions revealed that albumin and globulin were made up of sub‐units of different molecular weights ranging from 7 to 96 kDa. Water and oil absorption of the protein concentrates varied from 1.15 to 2.75 g g−1 and from 2.60 to 5.65 g g−1 respectively. Foaming capacity and foam stability of the protein concentrates were good and improved with the addition of salt (10 g L−1 NaCl) or sugar (100 g L−1 sucrose) at both isoelectric and neutral pH. Emulsifying capacity and emulsion stability of the protein concentrates were good and excellent respectively.CONCLUSION: Protein concentrates prepared from chickpeas have potential use in food formulations owing to their good emulsifying/foaming and water/oil‐binding capacities. Copyright © 2008 Society of Chemical Industry
Nurse dose: What's in a concept?
Authors: Manojlovich Milisa , Sidani Souraya 
Published: January 2008

AbstractMany researchers have sought to address the relationship between nursing care and patient outcomes, with inconsistent and contradictory findings. We conducted a concept analysis and concept derivation, basing our work on theoretical and empirical literature, to derive nurse dose as a concept that pulls into a coherent whole disparate variables used in staffing studies. We defined nurse dose as the level of nursing reflected in the purity, amount, frequency, and duration of nursing care needed to produce favorable outcomes. All four parameters of nurse dose used together can facilitate our understanding of how nursing contributes to patient outcomes. Ongoing investigation will help to identify the parameters of nurse dose that have the greatest effect on outcomes. © 2008 Wiley Periodicals, Inc. Res Nurs Health 31:310–319, 2008
Translational research: Essential contributions from interpretive nursing science
Authors: Chesla Catherine A. 
Published: January 2008

AbstractTranslating research into practice is a central priority in the National Institutes of Health Roadmap. Nurse scientists must reevaluate how proposed research fits within and contributes not only to the development of new knowledge, but to the translation of that knowledge to the care of diverse communities and populations. Key barriers to the translation of evidence based practices in health programs and initiatives have been identified in the health services and nursing literature. Contributions that interpretive research approaches enable in addressing those barriers are articulated. © 2008 Wiley Periodicals, Inc. Res Nurs Health 31:381–390, 2008
Dynamics of NGO legitimacy: how organising betrays core missions of INGOs
Authors: Ossewaarde Ringo , Nijhof André , Heyse Liesbet 
Published: January 2008

AbstractInternational non‐governmental organisations (INGOs) are prominent actors in the international arena, aiming to improve the life of disadvantaged people. However, INGOs often do not succeed in doing this. Consequently, INGO legitimacy is regularly questioned. Increased transparency and tightened accountability mechanisms are often‐mentioned solutions to this problem. Based on an analysis of four dimensions of INGO legitimacy—normative, regulatory, cognitive and output legitimacy—we argue that this is not necessarily adequate. We conclude that INGO mission statements create a normative source of legitimacy, but that this, in itself, is not enough to ground INGO legitimacy: it also needs to be institutionalised and organised. However, as a result of power relations and resulting pressures for accountability and transparency, as defined by their external stakeholders, INGOs experience a permanent struggle to reconcile their mission with the requirements for regulatory, cognitive and output legitimacy. The more these stakeholders press for increased organisation of INGO work, the more the pursuit of the core objectives of INGOs is obstructed. We illustrate this argument with the case of the post‐Tsunami humanitarian intervention (2004/2005). Copyright © 2008 John Wiley & Sons, Ltd.