bluuebunny/crossref_metadata_2025_split huggingface dataset:
ADAMTS-13 regulates platelet adhesion under flow
Authors: Budde Ulrich , Angerhaus Dorothea , Lubetsky Aharon , Savion Naphtali , Seligsohn Uri , Varon David , Shenkman Boris 
Published: July 2006

SummaryADAMTS-13 cleaves large and ultra-large vonWillebrand factor multimers normally secreted by endothelial cells. Severe deficiency of this enzyme leads to thrombotic thrombocytopenic purpura (TTP). We applied the Impact-R system [Cone and plate(let) Analyzer, CPA] to determine optimal conditions for ADAMTS-13 function, to assess it’s activity in TTP patients and to distinguish inheritedTTP (inTTP) from acquiredTTP (acTTP). The role of ADAMTS-13 in platelet adhesion under different conditions was investigated applying recombinant forms of VWF and ADAMTS-13. rVWF was first treated by rADAMTS-13 either in solution or when immobilized on the surface of the well, under static or flow conditions, in the presence or absence of BaCl2. The resulting cleavedVWF fragments were then immobilized and served to assess type 3 von Willebrand disease whole blood platelet adhesion under flow. Maximal effect of the rADAMTS-13 (decrease of platelet adhesion in the absence compared to the presence of BaCl2), was observed when the rVWF was pre-immobilized and the cleavage step took place under flow (85%). Mixing plasma ofTTP patients with normal blood (1:3) yielded a 1.6 to 2-fold increase of platelet adhesion under flow compared to mixing normal plasma with normal blood, at shear rate range of 1,800–2,500s−1. Maximal increase of platelet adhesion was observed under 2,050s−1. Under these conditions, the extent of adhesion was similarly higher in patients with inTTP and acTTP versus control [surface coverage (SC) 14.5 ± 2.8% and 14.6 ± 2.5% vs. 7.4 ± 1.7%, respectively]. ADAMTS-13 activity measured by collagen-binding test was similarly low (4.2 ± 3.8% and 3.5 ± 2.4% vs. 72.2 ± 8.0%, respectively). An inverse correlation between SC and ADAMTS-13 activity was observed in a patient with inTTP assayed eight times during plasma infusion treatment. Addition of BaCl2 to the mixture of TTP plasma and normal whole blood yieldeda decrease in platelet adhesion in inTTP (by 51%) but not in acTTP. The lack of reduction of platelet adhesion in acTTP could presumably be due to the presence of ADAMTS-13 inhibitor in these patients. These results suggest that VWF immobilization and high shear flow yielded optimal conditions for ADAMTS-13 activity, and that introduction of BaCl2 in the Impact-R (CPA) test may be useful for differentiation between inherited and acquired TTP.
Prevention effect of orally active heparin derivative on deep vein thrombosis
Authors: Kim Sang , Lee Dong , Kim Choong , Moon Hyun , Byun Youngro 
Published: July 2006

SummaryThe use of heparin as the most potent anticoagulant for the prevention of deep vein thrombosis and pulmonary embolism is nevertheless limited, because it is available to patients only by parenteral administration. Toward overcoming this limitation in the use of heparin, we have previously developed an orally active heparin-deoxycholic acid conjugate (LMWH-DOCA) in 10% DMSO formulation. The present study evaluates the anti-thrombogenic effect of this orally active LMWH-DOCA using a venous thrombosis animal model with Sprague-Dawley rats. When 5 mg/kg of LMWH-DOCA was orally administered in rats, the maximum anti-FXa activity in plasma was 0. 35 ± 0. 02, and anti-FXa activity in plasma was maintained above 0. 1 IU/ml [the minimum effective anti-FXa activity for the prevention of deep venous thrombosis (DVT) and pulmonary embolism (PE)] for five hours. LMWH-DOCA (5 mg/kg, 430 IU/kg) that was orally administered reduced the thrombus formation by 56. 3 ± 19. 8%;on the other hand, subcutaneously administered enoxaparin (100 IU/kg) reduced the thrombus formation by 36. 4 ± 14. 5%. Also, LMWH-DOCA was effectively neutralized by protamine that was used as an antidote. Therefore, orally active LMWH-DOCA could be proposed as a new drug that is effective for the longterm prevention of DVT and PE.
Leukemic lymphoblasts, a novel expression site of coagulation factor XIII subunit A
Authors: Veszprémi Anikó , Katona Éva , Kiss Csongor , Vereb György , Muszbek László , Kiss Flóra , Hevessy Zsuzsanna , Kappelmayer János 
Published: July 2006

SummaryBlood coagulation factor XIII (FXIII) is a protransglutaminase circulating asa tetramer formed by two types of subunits (A2B2). The intracellular dimeric form of FXIII (A2) is present in platelets, megakaryocytes, monocytes and macrophages and has been detected in mono-and megakaryocytic leukemias. The aim of our study was to investigate FXIII-A expression in newly diagnosed B cell acute lymphoblastic leukemia (ALL) samples. We examined 47 de novo ALL cases of B cell origin by triple color labeling with flow cytometry. FXIII-A was detected by a FITC conjugated monoclonal antibody combined with CD34 and CD45 staining. In selected cases FXIII-A was investigated on slides prepared from blasts and visualized with a fluorescent microscope. In addition, blasts were studied by Western blot analysis and FXIII-A was measured by a highly sensitive ELISA method. By flow cytometry 19 samples of the 47 cases were found to be FXIII-A positive. Antigen concentration was 3.11± 1.19 fg/blast, while normal lymphoid precursors and mature lymphocytes from B-CLL did not contain FXIII-A. In the lysate of lymphoblasts that were positive by flow cytometry, a single band (82 kDa) corresponding to FXIII-A was detected on Western blots. Confocal laser scanning microscopic examination revealed the presence of FXIII-A in the cytoplasm of these lymphoblasts. This novel expression site of FXIII-A in leukemic lymphoblasts can be utilized as a diagnostic tool and may also gain functional significance in B-lineage ALL.
Lipid raft adhesion receptors and Syk regulate selectin-dependent rolling under flow conditions
Authors: Abbal Claire , Lambelet Martine , Bertaggia Debora , Gerbex Carole , Martinez Manuel , Arcaro Alexandre , Schapira Marc , Spertini Olivier 
Published: July 2006

AbstractSelectins and their ligand P-selectin glycoprotein ligand-1 (PSGL-1) mediate leukocyte rolling along inflamed vessels. Cell rolling is modulated by selectin interactions with their ligands and by topographic requirements including L-selectin and PSGL-1 clustering on tips of leukocyte microvilli. Lipid rafts are cell membrane microdomains reported to function as signaling platforms. Here, we show that disruption of leukocyte lipid rafts with cholesterol chelating agents depleted raft-associated PSGL-1 and L-selectin and strongly reduced L-, P-, and E-selectin–dependent rolling. Cholesterol repletion reversed inhibition of cell rolling. Importantly, leukocyte rolling on P-selectin induced the recruitment of spleen tyrosine kinase (Syk), a tyrosine kinase associated to lipid raft PSGL-1. Furthermore, inhibition of Syk activity or expression, with pharmacologic inhibitors or by RNA interference, strongly reduced leukocyte rolling on P-selectin, but not on E-selectin or PSGL-1. These observations identify novel regulatory mechanisms of leukocyte rolling on selectins with a strong dependency on lipid raft integrity and Syk activity.
Antitumor Effects of the Proteasome Inhibitor Bortezomib in Medullary and Anaplastic Thyroid Carcinoma Cells in Vitro
Authors: Mitsiades Constantine S. , McMillin Douglas , Kotoula Vassiliki , Poulaki Vassiliki , McMullan Ciaran , Negri Joseph , Fanourakis Galinos , Tseleni-Balafouta Sophia , Ain Kenneth B. , Mitsiades Nicholas 
Published: July 2006

Abstract
Context: The ubiquitin-proteasome pathway is a major pathway for degradation of intracellular proteins. Proteasome inhibitors constitute a novel class of antitumor agents with preclinical and clinical evidence of activity against hematological malignancies and solid tumors. The proteasome inhibitor bortezomib (PS-341, Velcade) has been approved by the Food and Drug Administration for the treatment of multiple myeloma and is being studied intensely in several other malignancies. Its mechanism of action is complex but appears to include the inhibition of inhibitory-κB degradation, which leads to inactivation of the transcriptional factor nuclear factor-κB (NF-κB). NF-κB has been implicated in the pathophysiology of the most aggressive forms of thyroid carcinoma, i.e. medullary and anaplastic.
Objective and Methods: We evaluated the effect of bortezomib on a panel of thyroid carcinoma cell lines, originating from papillary, follicular, anaplastic, and medullary carcinomas.
Results: Bortezomib induced apoptosis in medullary and anaplastic cell lines with IC50 values well within the range of clinically achievable concentrations and much lower than respective IC50 values for other solid malignancies. Bortezomib inhibited NF-κB activity; increased p53, p21, and jun expression; and induced caspase-dependent apoptosis. Sensitivity of thyroid carcinoma cells to bortezomib was partially decreased by overexpression of Bcl-2 or treatment with IGF-I, whereas the combination of bortezomib with chemotherapy (doxorubicin) was synergistic.
Conclusions: These data provide both insights into the molecular mechanisms of antitumor activity of proteasome inhibitors and the rationale for future clinical trials of bortezomib, alone or in combination with conventional chemotherapy, to improve patient outcome in medullary and anaplastic thyroid carcinomas.
A SIMPLE MANOMETER FOR MEASURING LOW PRESSURES
Authors: Shirai Toshiaki 
Published: July 2006

Abstract
A simple all glass manometer, which can be used for measuring the pressures from 0.1 mm. to 0.001 mm. has been described. The manometer consists of a thermometer enclosed in a tube. The pressure measurement is done by measuring the time required to cool the thermometer from a given temperature to the other under a given condition. Using a thermometer of long mercury bulb and limiting the operating temperature range so narrow that the Newton’s law of cooling holds, we get the following relation:
(Remark: Graphics omitted.)
This relation was well confirmed by experiments using the air and the hydrogen.
DETERMINATION OF HIGH TEMPERATURE BY THE EFFUSION AND THE TRANSPIRATION OF GAS
Authors: Yamaguchi Yohei 
Published: July 2006

Abstract
Three methods have been studied for the purpose of determining high temperature by the observation of the rate of flow of gas. In the first method the temperature is determined by the effusion of gas, time necessary for the escape of a definite quantity of gas being observed. The following equation can be applied for the range from the common temperature up to about 1000°, t=a+b\sqrtT, where T is the temperature, t is the time of effusion of air which passes through a quartz opening, and a and b are constants. This method might be useful, as the apparatus employed for this purpose is very simple and the relation between time and temperature can be represented by a simple equation.
In the second method, the temperature is measured by the transpiration of gases through a quartz capillary, the time of flow being measured also in this case. In the range from common temperature up to about 1000°, the logarithms of the absolute temperature can be expressed as an equation of second order of the logarithms of the time.
In the third method, the increase of the pressure necessary to maintain a constant rate of flow was observed. In the range from the common temperature up to about 1200°, there was found a linear relation between the logarithm of pressure and that of absolute temperature.
THE MOLECULAR ASSOCIATION OF PHENOL IN BENZENE AND WATER
Authors: Endo Kwantaro 
Published: July 2006

Abstract
1. From the measurement of the partition coefficient of phenol between benzene and water, it has been proved that phenol is in the state of chemical equilibrium,
3C6H5OH \leftrightarrows (C6H6O)3,
while in the benzene layer at 25°C.
2. From the measurement of the freezing point of the aqueous phenol solution, it has also been proved that phenol is in the chemical equilibrium similar to the above in the aqueous solution at 0°C.
It is to be emphasized that phenol associates itself in tripple molecules and not in double molecules.
ON THE INDUCED REACTION
Authors: Horiuchi Riki 
Published: July 2006

Abstract
(1) The induced reaction, which was formerly limited to oxidation reaction, has been extended to reactions in general, and many examples have been given.
(2) All these induced reactions have been proved to be catalytic actions.
(3) Evidences have been given to show that among catalytic actions, positive or negative, and promotor actions, there are many instances which can be described as induced reactions.
(4) It has been shown that these induced reactions must always have two similar substances as inducer and acceptor.
PRECIPITATION OF ALUMINIUM WITH HYDROGEN AMMONIUM CARBONATE
Authors: Kozu Toshio 
Published: July 2006

Abstract
(1) Hydrogen ammonium carbonate prepared by saturating the solution of commercial ammonium carbonate with carbon dioxide may conveniently be used for quantitative precipitation of aluminium.
(2) The precipitate formed with hydrogen ammonium carbonate at ordinary temperature is fairly stable in a solution having the pH value of 7.6, and its composition is represented by 4Al(OH)CO3·6Al(OH)3·9H2O.
(3) The solubility of the basic carbonate in water is found to be 0.00242 g. per 1000 c.c. at 20–21°.
(4) The analytical procedure for gravimetric determination of aluminium with hydrogen ammonium carbonate is given.
(5) Several precautions which are to be taken in the analytical treatment of basic aluminium carbonate are fully described.
In conclusion the author wishes to express his warm thanks to Prof. Motooki Matsui for his kind guidance and valuable suggestions.
ON THE ARRANGEMENTS OF THE MICRO-CRYSTALS IN LEAD DEPOSITED BY ELECTROLYSIS
Authors: Hirata Hideki , Tanaka Yoshio , Komatsubara Hisaji 
Published: July 2006

Abstract
The arrangements of the micro-crystals in electrolytic specimens deposited from acetic acid solutions, were examined with X-rays, by the so-called “transmission method”. From the diffraction patterns obtained, it was confirmed on the one hand, that the micro-crystals of lead have a tendency to be electrolytically deposited in a fibrous way, with one of the normals to their (211) faces arranged parallel to a definite common direction, as was the case in the experiments of Frölich, Clark, and Aborn. On the other hand, the direction of the growth of deposited lead, was found to coincide roughly with any one of the [211] axes, which make an angle 0°, 33° 34′, 48° 12′, and 80° 24′ respectively with the common axis of the micro-crystals.
THE HIGHLY UNSATURATED ACIDS IN SARDINE OIL. VII. THE SEPARATION OF HIGHLY UNSATURATED C22-ACIDS
Authors: Toyama Yoshiyuki , Tsuchiya Tomotaro 
Published: July 2006

Abstract
A concentrated fraction of highly unsaturated acids has been separated from sardine oil by means of sodium-soap-acetone method. It was converted into methyl esters and the latter subjected to a fractional distillation which yielded a fraction consisting of the methyl esters of C22-acids. This fraction and the free fatty acids liberated from it showed iodine values which were close to those of methyl clupanodonate and clupanodonic acid respectively, but on separating the fatty acids of this fraction by a fractional precipitation of sodium soap in acetone solution, they were found to contain, in addition to clupanodonic acid, some acids of different degrees of unsaturation. After a repeated separation, clupanodonic acid has been separated in much purer state than prepared hitherto, and also a more highly unsaturated portion consisting chiefly of docosahexenoic acid C22H32O2 has been separated. There was obtained also a less unsaturated portion than clupanodonic acid; this was, however, found to be a mixture contaminated with cetoleic acid C22H42O2, though in a minor amount. Docosatetraenoic acid C22H36O2 was not separated, although this acid could not be altogether deemed to be absent.
SURFACE POTENTIAL DIFFERENCES OF UNIMOLECULAR FILMS OF FATTY ACIDS
Authors: Yamaguchi Yohei , Mizuno Saburo 
Published: July 2006

Abstract
(1) Gas—liquid interfacial potential difference of fatty acid film was measured by using the method of ionizing air gap between the electrode and the surface of the liquid with X-ray.
(2) Various factors which affect the measurements were studied at first.
(3) Under the most favourable conditions the measurements of the surface potentials were carried out with four fatty acids, i.e. myristic, palmitic, stearic, and oleic acids.
(4) The surface potential difference attains a maximum or a saturation value at a certain definite surface concentration. This value is nearly equal for three saturated fatty acids and is about 350 mv. The value for oleic acid, however, is about 240 mv.
(5) The (surface potential)—(surface concentration)-curves were plotted and the mean vertical component of dipole moment was calculated for these fatty acids by applying Helmholtz’s formula.
(6) The break point of the curve or the saturation point of fatty acid is nearly similar for four acids, and the area occupied by a molecule at this point is about 27 sq. Å.
ON THE ABSORPTION OF HYDROGEN CHLORIDE INTO VARIOUS ORGANIC LIQUIDS AND CALCULATION OF THE HEAT OF ABSORPTION. I
Authors: Hamai Senzo 
Published: July 2006

Abstract
(1) Absorption of hydrogen chloride into CCl4, C2H4Cl2, C2H2Cl4 (1,1, 2,2), and C2H4Br2 at 15°, 20°, and 25°C. has been studied.
(2) The heats of absorption of hydrogen chloride into these solvents were calculated.
(3) The relations between the solubility and polarity of organic liquids involved, and also the internal pressure have been discussed.
(4) The solubilities of hydrogen chloride in these liquids have been found in the order of increasing bond energies of the liquids calculated similarly as Pauling has done, and a possible explanation of it has been suggested.
SPECTROSCOPIC STUDIES OF LUMINESCENCE AT THE CATHODE DURING ELECTROLYSIS
Authors: Uhara Iturô 
Published: July 2006

Abstract
If the current density at the cathode is high enough for the formation of a thin film of hydrogen in the electrolysis of the electrolyte solutions with platinum wire electrodes, considerably intense cathode luminescence takes place; and the spectra are in most cases the same as the arc spetra (sometimes spark spectra) of the elements forming cations, and further Balmer lines of H and the molecular spectra of H2 are often observed.
TETRADECENOIC AND DODECENOIC ACIDS IN SPERM OIL. II. DODECENOIC ACID IN SPERM HEAD OIL
Authors: Toyama Yoshiyuki , Tsuchiya Tomotaro 
Published: July 2006

Abstract
Dodecenoic acid C12H22O2 has been isolated from sperm head oil, and methyl dihydroxylaurate C13H26O4 prepared from it has been subjected to permanganate oxidation in acetone. On examining the oxidation products the constitution of dodecenoic acid has been proved to be CH3·(CH2)5·CH=CH·(CH2)3·COOH. It is thus seen that dodecenoic acid in sperm head oil is identical with denticetic acid (Δ5:6-dodecenoic acid) in sperm blubber oil described in the preceding paper.
ORGANIC SALTS CONTAINING IRON AND CALCIUM
Authors: Harada Taichi 
Published: July 2006

Abstract
One molecular proportion of calcium lactate appears to react with one molecular proportion of ferric citrate in aqueous solution, producing a salt-like substance having the formula, FeCaC9H11O11, according to the following reaction:
Fe(C6H5O7)+Ca(C3H5O3)2+H2O=Fe(C6H5O7)Ca(OH)C3H5O3+C3H6O3.
THE ELECTROLYTIC FORMATION OF PERSULPHATE. PART I
Authors: Matsuda Ryoichi 
Published: July 2006

Abstract
1. Sulphuric acid and ammonium sulphate solutions were electrolysed in order to see the influences of the current density and OH′-concentration.
2. Influence of various cations, H., K., NH4., Na., Li.., Mg.., Zn..., FE..., Al..., Cu.., and Cd.., was studied and some were explained to have connexion with persulphate formation.
3. A new suggestion was made that OH′ plays an important rôle in persulphate formation, and this suggestion will clearly explain the results of the present investigation which may otherwise be obscure.
ON THE COMPOSITION OF RAPE SEED OIL
Authors: Yamasaki Riichiro , Ichihara Kentaro 
Published: July 2006

Abstract
The composition of rape seed oil was determined and the contents of the fatty acids were calculated from the chemical numbers of the original oil and each fraction: behenic acid 0.8%, erucic acid 55%, oleic acid 14%, linolic acid 24%, linolenic acid 2%, and palmitic acid 3.5%. Myristic, palmitoleic, and stearic acids, however, could not be found
A NEW BACTERIOXANTHOPHYLL, THE YELLOW PIGMENT OF SARCINA LUTEA
Authors: Nakamura Teruko 
Published: July 2006

Abstract
The yellow pigment of Sarcina lutea was examined. It is a lipochromic substance with absorption maxima at 490,460,and 433 mμ in carbon bisulphide apparently distinct from well known xanthophylls, the constitution of which had not been identified. Saponification showed the substance to be chiefly a xanthophyll-like ester. The work is being continued.
ON THE FORMATION OF SYMMETRICAL DIBENZO-CYCLO-DOCOSANE-DIONE
Authors: Uenaka Mioji , Kubota Bennosuke 
Published: July 2006

Abstract
The synthesis of an eleven-membered carbon ring attached to the ortho-position of benzene was attempted. The dry distillation of the thorium salt of o-phenylene-divaleric acid resulted in the formation of a twenty-two-membered diketone ring. Several new compounds incidental to the synthesis are reported.
THE HIGHLY UNSATURATED ALCOHOLS IN SPERM BLUBBER OIL
Authors: Toyama Yoshiyuki , Akiyama Goroku 
Published: July 2006

Abstract
The high boiling fraction of the unsaponifiable matter of sperm blubber oil was brominated in ethereal solution, and the ether insoluble bromide was separated and then debrominated to regenerate the highly unsaturated alcohols. These were converted into acetates, and the latter were fractionated. An examination of the resulting fractions showed that the highly unsaturated alcohols of sperm blubber oil contained two individual alcohols C20H34O and C22H36O as chief components.
STUDIES ON THE RAMAN EFFECT OF ORGANIC SUBSTANCES. VII. RAMAN EFFECT OF FURANE DERIVATIVES
Authors: Han Kwan 
Published: July 2006

Abstract
(1) The Raman spectra of the following substances have been measured: α-furfuryl methyl ether, α-furfuryl ethyl ether, α-furfuryl-acetone, α-furyl-ethylene, α-furyl cyanide, 2,5-dimethyl-furane, 2-methyl-furyl cyanide-(5) and ethyl 2-methyl-furoate-(3).
(2) The characteristic frequencies of the α-furfuryl-radical are confirmed. The difference of the Raman spectra of the mono-and di-derivatives of furane is established.
(3) The constitutive influences exerted on the Raman frequencies in the region between Δν 1400 and 1600 cm.−1 are observed.
(4) The Raman lines at 1642 and 1292 cm.−1 in α-furyl-ethylene are associated with the structure of R–CH:CH2, and those at 183, 570, and 2233 cm.−1 in α-furyl cyanide with the structure of R–C≡N, when R is taken as furyl radical.
THE HIGHLY UNSATURATED ACIDS IN SARDINE OIL. XII. A FURTHER EXPERIMENT ON THE SEPARATION OF OCTADECATRIENOIC ACID C18H30O2
Authors: Toyama Yoshiyuki , Tsuchiya Tomotaro 
Published: July 2006

Abstract
After having found in a previous study that sardine oil contains an octadecatrienoic acid as a minor constituent of highly unsaturated C18-acids, we have attempted in the present experiment to separate the octadecatrienoic acid starting with a large amount of sardine oil. The results of the present experiment seem to indicate that the octadecatrienoic acid giving an etherinsoluble bromide is contained only in far lesser proportion than presumed before.