bluuebunny/crossref_metadata_2025_split huggingface dataset:
RANCANG BANGUN SISTEM PENGENDALI SUHU DAN KELEMBABAN PADA KUMBUNG JAMUR TIRAM BERBASIS ARDUINO
Authors: Imron Muhammad , Mahmuri Mahmuri 
Published: February 2024

Jamur tiram adalah salah satu dari spesies jamur yang membutuhkan kondisi lingkungan khusus dan berbeda jika dibandingkan dengan tumbuh-kembangan lainnya. Suhu dan kelembaban adalah faktor penting yang dapat mempengaruhi tumbuh-kembang-kembangnya jamur tiram. Pertumbuh-kembangan jamur tiram akan optimal jika berada pada kondisi suhu 22 – 28 ºC dan kelembaban 80 – 95 %. Budidaya jamur tiram yang ada dalam kumbung adalah upaya mengendalikan suhu dan kelembaban optimal untuk pertumbuh-kembangan jamur. Saat ini pengendalian suhu dan kelembaban dalam kumbung ternyata masih banyak dilakukan secara manual, hal ini dianggap kurang efektif sehingga akan berdampak pada produktifitas jamur tiram. Perkembangan teknologi yang ada memberikan pilihan lain untuk menciptakan sistem control suhu dan kelembaban dalam kumbung jamur secara otomatis. Maka dari itu, dalam penelitian ini dirancang sebuah sistem untuk mengendalikan suhu dan kelembaban dalam kumbung jamur secara otomatis berbasis Arduino Uno. Penelitian ini menyajikan sistem pengendali suhu dan kelembaban pada kumbung jamur tiram yang berbasis Arduino Uno yang dapat meningkatkan efektifitas control suhu dan kelembaban pada kumbung jamur yang dalam hal ini lebih mudah dalam pengoperasian, praktis dalam perakitan, dan memiliki perawatan yang lebih mudah. Sistem ini dapat membantu para pembudidaya jamur tiram baik yang terlatih maupun yang belum. Pada penelitian berikut ini pengujian dan pengambilan data dilakukan dengan menggunakan prototype kumbung jamur. Kata kunci:Jamur tiram, Suhu, Kelembaban, Prototype kumbung jamur, Arduino Uno.
Система мотивов в лирике Б. Канапьянова
Authors: Нұрғали Қ.Р. , Амангелді М.Д. 
Published: February 2024

В статье исследована система мотивов поэтического языка казахстанского поэта Б. Канапьянова, представленная в его лирических произведениях. «Мотивная система» рассмотрена в совокупности ее содержательного и структурного компонентов. Основная задача работы — описать устойчивые мотивы через способы их создания и развития в поэтическом тексте. В качестве исходной позиции принимается существование мотива как в буквальном, так и в образном выражении. В зависимости от способа выражения мотива в тексте выбирается характер его описания. Следует подчеркнуть, что изучение мотивной системы, безусловно, предполагает обращение как к авторской проблематике в общем, так и к конкретному образному ряду. Авторами предпринята попытка определить специфику мотивов лирики Б. Канапьянова в сравнении с общекультурными мотивами, выявить и классифицировать способы и средства их репрезентации в художественных текстах. Важное место занимает проблема структурирования таких субконцептосфер, как «семья», «Родина», «степь», «аул». Выделены их составляющие и определены систематические связи между ними, вычленены и классифицированы основные языковые средства их репрезентации. В результате анализа сделан вывод, что мотивы выполняют различные функции: от характеристики лирической ситуации до образа, ключевой темы стихотворения. Диапазон осмысления системы мотивов и ее художественного выражения достаточно широк и разнообразен. Их анализ позволяет глубже понять мировосприятие автора, которое воплощается в его поэзии, и вывести осмысление его творчества на новый уровень.
PERTURBAÇÕES ANTRÓPICAS E POLUIÇÃO POR LIXO MARINHO NO LITORAL SUL DE ARACAJU: COMPORTAMENTO SOCIAL E AÇÕES PÚBLICAS COMO FATORES DE DEGRADAÇÃO AMBIENTAL
Authors: Knupp de Brito Elaine , Manso Raimundo da Rocha Jorge Alberto 
Published: February 2024

O descarte irregular de lixo em ambientes marinhos ou terrestres, a contaminação ambiental, a urbanização desenfreada e o trânsito de veículos automotores na faixa de areia representam riscos à vida e à saúde de seres humanos e animais marinhos. Este estudo teve como objetivo quantificar e classificar os resíduos marinhos antropogênicos encalhados na praia, além de identificar qualitativamente outras fontes de perturbações antrópicas no litoral sul de Aracaju (Sergipe, Brasil). Os resultados apontam para o aumento de 229,8% no volume de resíduos sólidos antropogênicos na faixa de areia em período sem restrições de acesso relacionados à pandemia de covid-19, quando comparado a um período com acesso restrito à praia. Os resíduos plásticos representaram 88,6% do volume total de lixo marinho e as atividades de turismo e recreação nas praias foram as principais fontes de descarte. Outros impactos antrópicos relacionados a comportamentos sociais foram identificados, como o tráfego de veículos automotores nas praias e as ações ineficientes de serviços públicos que colaboraram para o acúmulo de resíduos sólidos na área costeira. Todos os registros mapeados têm em comum atos de desordem que geram a percepção de deterioração e de ausência de leis, normalizando comportamentos nocivos de grande impacto na degradação do ecossistema marinho.
Palavras-chave: resíduos antropogênicos, poluição marinha, lixo marinho, região Nordeste do Brasil, poluição por plásticos
ENFERMIDADES VIRAIS EM LAGOSTAS DO GÊNERO Panulirus: UMA REVISÃO SISTEMÁTICA
Authors: Da Silveira Siqueira Ingrid Luana , Andrade Thays , Dos Santos Rocha Rafael , De Carvalho Gaeta Juliana , Maggioni Rodrigo 
Published: February 2024

Lagostas do gênero Panulirus são um importante recurso para a pesca mundial. No entanto, como todos os crustáceos marinhos, são acometidas por uma variedade de enfermidades causadas por vírus, bactérias e parasitas. O impacto das enfermidades sobre a disponibilidade desse recurso pesqueiro ainda é pouco estudado e é possível que seja magnificado pelas mudanças climáticas globais em curso. Esta revisão sistemática abordou as patologias virais que infectam lagostas do gênero Panulirus, tendo como objetivo compilar as informações acerca dos sinais clínicos dessas enfermidades, suas características nos tecidos dos animais infectados e as metodologias empregadas para o seu estudo. Até o momento, apenas um vírus específico de lagostas do gênero Panulirus foi identificado e descrito, o Panulirus argus vírus (PaV1). Além dele, já foi registrado nas lagostas espinhosas o vírus da mancha branca (WSSV), que causa uma enfermidade grave em camarões marinhos.
Palavras-chave: Palinuridae, WSSV, PaV1, crustáceos.
CONSTRUINDO UM INDICADOR DE ACESSIBILIDADE AOS PORTOS PÚBLICOS BRASILEIROS
Authors: da Rocha Gonçalves Rodrigo , Pinto Guilherme , Ávila Santos Regina , Da Silva Noda Michel 
Published: February 2024

Os portos desempenham um papel estratégico no crescimento econômico do Brasil. O aumento das importações e exportações marítimas tem gerado uma crescente demanda por esses serviços, porém, a ineficiência logística e de transporte tem prejudicado as operações portuárias. Dessa forma, este artigo desenvolve um índice abrangente de acessibilidade para os portos brasileiros considerando fatores como profundidade do calado, conectividade com outros modais de transporte, distância até as capitais estaduais e qualidade das rodovias de acesso, com o objetivo de identificar os portos com a melhor acessibilidade no país. Dentre os resultados identificou-se que os portos mais acessíveis incluem Santos, Itaguaí, Rio de Janeiro, Paranaguá, São Sebastião e Itaqui, com um índice de acessibilidade superior a 0,60. Por outro lado, portos como Areia Branca, Ilhéus, Santana, Maceió, Recife, Aratu e Santarém apresentaram os piores resultados, com índices abaixo de 0,40. Isso destaca a importância da infraestrutura sólida e do potencial de mercado para melhorar a acessibilidade. Além, foi observado a necessidade de investimentos na infraestrutura de transporte aquaviário, especialmente na profundidade do calado. Portos como Pelotas, Porto Alegre e Estrela foram identificados com os menores calados, registrando índices de acessibilidade extremamente baixos (0,03 e 0,00). Esses resultados têm implicações importantes para orientar futuros investimentos e melhorias na infraestrutura portuária brasileira, visando aprimorar a acessibilidade e estimular o comércio marítimo do país.Palavras-chave: Índice de Acessibilidade; Brasil; Nova Geografia Econômica.
PLASTIC DEBRIS USAGE BY TUBE-BUILDING POLYCHAETE Diopatra cuprea COMPLEX
Authors: Cruz da Rosa Leonardo 
Published: February 2024

In this short note the plastic debris usage by tube-building polychaete Diopatra cuprea complex in the tube caps decoration is reported for the first time. Soft plastics attached to tube caps were recorded at a tidal flat in the Sergipe River estuary, northeastern Brazil. This interaction probably reflects an adaptation of this polychaete to a highly polluted environment.
Keywords: pollution, soft plastics, tube caps, decoration.
Evaluation of transfection with gemini nanoparticles for retinal neuroprotection in a dynamic microfluidic cell culture system
Authors: Narsineni Lokesh , Hakim Antoine , Guido Benjamin , Foldvari Marianna 
Published: February 2024

<img src=” https://s3.amazonaws.com/production.scholastica/article/94464/large/prnano_1252024ga.jpg?1709170827”> Glaucoma is a neurodegenerative disease of the retina and optic nerve with elevated intraocular pressure as one of the main contributing factors. The traditional in vitro methods to evaluate new gene delivery systems for neuroprotective treatments of glaucoma require testing in cell monolayers under static conditions. Therefore, a microfluidic technology that provides a dynamic simulation of cell exposure to nanoparticles (NPs) could be a useful model for rapid screening. In this study, CellASIC ONIX2 microfluidic platform was used to evaluate and optimize culture and transfection conditions to assess cell viability and transfection efficiency (TE) of gemini NPs carrying GFP-encoding plasmid in A7 astrocytes, one of the targeted retinal cells for glaucoma treatment. The average TE (inlet-middle-outlet of culture chambers) by gemini nanoplexes, 18-7NH-18 NPXs and 18-7Np3-18 NPXs was > 85% and > 25%, respectively, after 5 h of perfusion with a combined average FI (caFI) of about 600 and < 100, respectively, whereas TE after Lipofectamine 3000 was > 70% and caFI of < 100. The results have shown that comparatively to 2D monolayers, the microfluidics technique provides better exposure of cells to NPs, suitable for kinetic mapping of gene expression, and a useful tool for cell growth and live evaluation of gene delivery system interaction with cells.
A new bird calicivirus detected in feces of cormorants
Authors: Wu Yan , Lu Chunying , Zhao Ran , He Yuyun , Hou Jingjing , Sun Yijie , Yang Shixing , Qin Zhaoying , Ji Likai , Wang Yan , Zhang Wen 
Published: February 2024

The incidence of reporting caliciviruses in wild birds is less common than in other animals, and the majority of cases remain unclassified. A strain of calicivirus was discovered in this study in the feces of cormorants collected at Xiamen Horticulture Expo Garden in 2021 and was named Cormcali01. The genome of Cormcali01 was 8,561 bp in length which contained characteristic motifs present in other caliciviruses. Furthermore, it demonstrated a significant deviation from all existing calicivirus nucleotide sequences, exhibiting the highest amino acid identity (47.34%) to the unclassified Ruddy turnstone calicivirus A. A pairwise comparison of the VP1 protein showed that Cormcali01 had the highest amino acid identity of 43.90% with the unassigned Ruddy turnstone calicivirus A. Phylogenetic analysis demonstrated that VP1 of Cormcali01 clustered with unassigned caliciviruses. Therefore, based on phylogenetic analysis and pairwise comparison, Cormcali01 should be affiliated with the unassigned calicivirus, which were suggested to comprise a new calicivirus genus, the Sanovirus genus. After investigating the prevalence of Cormcali01, we discovered that 22.22% of fecal samples (10/45) were tested positive. These findings expand our understanding of the genetic variation of caliciviruses and provide valuable epidemiological information regarding a potential outbreak of calicivirus disease in birds.
Relevance of mutations in protein deubiquitinases genes and TP53 in corticotroph pituitary tumors
Authors: Pękul Monika , Szczepaniak Magdalena , Kober Paulina , Rusetska Natalia , Mossakowska Beata J. , Baluszek Szymon , Kowalik Artur , Maksymowicz Maria , Zieliński Grzegorz , Kunicki Jacek , Witek Przemysław , Bujko Mateusz 
Published: February 2024

IntroductionCorticotroph pituitary neuroendocrine tumors (PitNETs) develop from ACTH-producing cells. They commonly cause Cushing’s disease (CD), however, some remain clinically silent. Recurrent USP8, USP48, BRAF and TP53 mutations occur in corticotroph PitNETs. The aim of our study was to determine frequency and relevance of these mutations in a possibly large series of corticotroph PitNETs.MethodsStudy included 147 patients (100 CD and 47 silent tumors) that were screened for hot-spot mutations in USP8, USP48 and BRAF with Sanger sequencing, while 128 of these patients were screened for TP53 mutations with next generation sequencing and immunohistochemistry.ResultsUSP8 mutations were found in 41% CD and 8,5% silent tumors, while USP48 mutations were found in 6% CD patients only. Both were more prevalent in women. They were related to higher rate of biochemical remission, non-invasive tumor growth, its smaller size and densely granulated histology, suggesting that these mutation may be favorable clinical features. Multivariate survival analyses did not confirm possible prognostic value of mutation in protein deubiquitinases. No BRAF mutations were found. Four TP53 mutations were identified (2 in CD, 2 in silent tumors) in tumors with size &gt;10mm including 3 invasive ones. They were found in Crooke’s cell and sparsely granulated tumors. Tumors with missense TP53 mutations had higher TP53 immunoreactivity score than wild-type tumors. Tumor with frameshift TP53 variant had low protein expression. TP53 mutation was a poor prognostic factor in CD according to uni- and multivariate survival analyses in spite of low mutations frequency.ConclusionsWe confirmed high prevalence of USP8 mutations and low incidence of USP48 and TP53 mutations. Changes in protein deubiquitinases genes appear to be favorable prognostic factors in CD. TP53 mutations are rare, occur in both functioning and silent tumors and are related to poor clinical outcome in CD.
Responses of fungal diversity and community composition after 42 years of prescribed fire frequencies in semi-arid savanna rangelands
Authors: Poswa Sanele Briged , Manyevere Alen , Mashamaite Chuene Victor 
Published: February 2024

Prescribed fire frequencies have been widely used to reduce the risk of severe wildfire occurrences. In addition, several studies have been conducted to assess the impact of fire frequencies on vegetation, vertebrate, and invertebrate species, as well as soil physical and chemical properties. However, there is a lack of empirically based knowledge concerning the impact of fire frequency on soil microorganisms. This study assessed the effect of different fire frequencies on the diversity and composition of soil fungal communities in a semi-arid savanna rangeland. Soil samples were collected from an ongoing long-term trial at the University of Fort Hare (South Africa) on the following treatments: (i) no burning; (ii) annual burning (burned once every year); (iii) biennial burning (burned once every 2 years); (iv) triennial burning (burned once every 3 years); (v) quadrennial burning (burned once every 4 years); and (vi) sexennial burning (burned once every 6 years). Fungi were identified using high-throughput sequencing, with Shannon-Wiener and Inverse Simpson diversity indexes being used for diversity and network analysis. Principal coordinate analysis was used for Bray-Curtis distance matrices to visualise the relationships between treatments. The highest diversity was found in biennial burning, which was significantly different (p &lt; 0.05) from the sexennial, quadrennial, and no burning treatments but was not different from the triennial and annual burning treatments. Regarding the taxa, Ascomycota and Basidiomycota were the phyla with the highest relative abundance, followed by Mortierellomycota, Chytridiomycota, and Rozellomycota. The different fire frequencies had an influence on soil fungi diversity and taxonomic composition in semi-arid savanna rangelands.
Industrial agglomeration, international R&D capital and air pollution-based on provincial spatial panel data
Authors: Gao Dawei , Deng Yan , Chen Zhengyang 
Published: February 2024

Introduction: This paper presents a spatial effect model to examine the impact of industrial agglomeration and international R&amp;D capital technology spillover on air pollution.Methods: The study utilizes spatial panel data from 30 provinces and cities in China spanning the period 2008 to 2022, allowing for the decomposition of both direct and indirect spillover effects.Results: Additionally, the research investigates the threshold effect of industrial agglomeration and international research and development capital and technology spillover on air pollution, considering independent research and development as the threshold variable. The findings reveal evident spatial autocorrelation and spatial dependence between industrial agglomeration, international R&amp;D capital, and air pollution. Importantly, both international R&amp;D capital technology spillover and industrial agglomeration exhibit a single threshold effect. This implies that surpassing the threshold value leads to a more pronounced positive impact of international R&amp;D capital technology spillover on reducing air pollution, while the impact of industrial agglomeration on air pollution tends to be positive.Discussion: The findings of this study have significant theoretical and practical implications for the promotion of sustainable economic development, particularly in China.
Longitudinal rheumatoid factor autoantibody responses after SARS-CoV-2 vaccination or infection
Authors: Keijzer Sofie , Oskam Nienke , Ooijevaar-de Heer Pleuni , Steenhuis Maurice , Keijser Jim B.D. , Wieske Luuk , van Dam Koos P.J. , Stalman Eileen W. , Kummer Laura Y.L. , Boekel Laura , Kuijpers Taco W. , ten Brinke Anja , van Ham S. Marieke , Eftimov Filip , Tas Sander W. , Wolbink Gerrit J. , Rispens Theo 
Published: February 2024

BackgroundRheumatoid factors (RFs) are autoantibodies that target the Fc region of IgG, and are found in patients with rheumatic diseases as well as in the healthy population. Many studies suggest that an immune trigger may (transiently) elicit RF responses. However, discrepancies between different studies make it difficult to determine if and to which degree RF reactivity can be triggered by vaccination or infection.ObjectiveWe quantitatively explored longitudinal RF responses after SARS-CoV-2 vaccination and infection in a well-defined, large cohort using a dual ELISA method that differentiates between true RF reactivity and background IgM reactivity. In addition, we reviewed existing literature on RF responses after vaccination and infection.Methods151 healthy participants and 30 RA patients were included to measure IgM-RF reactivity before and after SARS-CoV-2 vaccinations by ELISA. Additionally, IgM-RF responses after a SARS-CoV-2 breakthrough infection were studied in 51 healthy participants.ResultsPublished prevalence studies in subjects after infection report up to 85% IgM-RF seropositivity. However, seroconversion studies (both infection and vaccination) report much lower incidences of 2-33%, with a trend of lower percentages observed in larger studies. In the current study, SARS-CoV-2 vaccination triggered low-level IgM-RF responses in 5.5% (8/151) of cases, of which 1.5% (2/151) with a level above 10 AU/mL. Breakthrough infection was accompanied by development of an IgM-RF response in 2% (1/51) of cases.ConclusionOur study indicates that de novo RF induction following vaccination or infection is an uncommon event, which does not lead to RF epitope spreading.
TIM-3, LAG-3, or 2B4 gene disruptions increase the anti-tumor response of engineered T cells
Authors: Cianciotti Beatrice Claudia , Magnani Zulma Irene , Ugolini Alessia , Camisa Barbara , Merelli Ivan , Vavassori Valentina , Potenza Alessia , Imparato Antonio , Manfredi Francesco , Abbati Danilo , Perani Laura , Spinelli Antonello , Shifrut Eric , Ciceri Fabio , Vago Luca , Di Micco Raffaella , Naldini Luigi , Genovese Pietro , Ruggiero Eliana , Bonini Chiara 
Published: February 2024

BackgroundIn adoptive T cell therapy, the long term therapeutic benefits in patients treated with engineered tumor specific T cells are limited by the lack of long term persistence of the infused cellular products and by the immunosuppressive mechanisms active in the tumor microenvironment. Exhausted T cells infiltrating the tumor are characterized by loss of effector functions triggered by multiple inhibitory receptors (IRs). In patients, IR blockade reverts T cell exhaustion but has low selectivity, potentially unleashing autoreactive clones and resulting in clinical autoimmune side effects. Furthermore, loss of long term protective immunity in cell therapy has been ascribed to the effector memory phenotype of the infused cells.MethodsWe simultaneously redirected T cell specificity towards the NY-ESO-1 antigen via TCR gene editing (TCRED) and permanently disrupted LAG3, TIM-3 or 2B4 genes (IRKO) via CRISPR/Cas9 in a protocol to expand early differentiated long-living memory stem T cells. The effector functions of the TCRED-IRKO and IR competent (TCRED-IRCOMP) cells were tested in short-term co-culture assays and under a chronic stimulation setting in vitro. Finally, the therapeutic efficacy of the developed cellular products were evaluated in multiple myeloma xenograft models.ResultsWe show that upon chronic stimulation, TCRED-IRKO cells are superior to TCRED-IRCOMP cells in resisting functional exhaustion through different mechanisms and efficiently eliminate cancer cells upon tumor re-challenge in vivo. Our data indicate that TIM-3 and 2B4-disruption preserve T-cell degranulation capacity, while LAG-3 disruption prevents the upregulation of additional inhibitory receptors in T cells.ConclusionThese results highlight that TIM-3, LAG-3, and 2B4 disruptions increase the therapeutic benefit of tumor specific cellular products and suggest distinct, non-redundant roles for IRs in anti-tumor responses.