bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imunomodulator Properties of White Turmeric (Curcuma Mangga Val.) in Vivo
Authors: Pujimulyani Dwiyati , Yulianto Wisnu Adi , Indarto Tri , Windrayahya Sulkhan 
Published: February 2024

Abstract
White turmeric contains bioactive compounds that have potential as immunomodulators. This research aims to assess the antioxidant properties and quantify the total phenolic content of white turmeric in vitro and test immunomodulatory properties in vivo. The study utilized a Completely Randomized Block Design (CRBD) with 2 treatment factors, namely the variation in parts of white turmeric rhizomes (main and tiller) and the variation in blanching time using the steam blanching method (0; 2.5; 5; 7.5; and 10 min). The analyses carried out were antioxidant activity of DPPH method (2,2 diphenyl-1-pickrylhidrazyl), and total phenolic content of white turmeric. Selected white turmeric powder based on in vitro test was analysed for immunomodulator (SOD (Superoxide Dismutase), IL-1 (Interleukin-1), IL-6 (Interleukin-6), IL-8 (Interleukin-8), IgE (Immunoglobulin E), IgG (Immunoglobulin G), dan IgM (Immunoglobulin M)). Selected white turmeric is the main rhizome with 5 min steam blanching. The analysis showed antioxidant activity of 81.46% RSA and total phenolic content of 6.08 mg EAG/g db. The results of in vivo studies showed that rats given white turmeric had an SOD value of 72.92% significantly better than rats given Na-CMC (Sodium Carboxymethyl Cellulose) and not significantly different from commercial supplements (stimuno). The IL-1 value of 0.40 pg/ml and IL-8 value of 48.53 pg/ml of rats given white kunir were significantly better than rats given Na-CMC and stimuno. The antibody values of IgE 74.02 ng/ml, IgG 18.20 ng/ml and IgM 2.97 ng/ml of rats treated with white turmeric were significantly better than those treated with Na-CMC and stimuno. The antibody values indicates that white turmeric has immunomodulatory effects.
Ameliorate Cardiovascular Condition through Positive Psychology and Lifestyle Intervention in China: Study Protocol of the ACCOMPLI-CH Randomized Controlled Trial
Authors: Liu Xiaomei , Zhai Boyu , Zhu Xinyi , Zheng Zhiwei , Yu Jing , Wang Baoxi , Zeng Hui , Jiang Lijuan , Li Chunbo , Liu Deping , Zhang Tiemei , Yao Yao , Yin Xiangjun , Li Juan 
Published: February 2024

Abstract

Background
Cardiovascular health is influenced by various factors including not only physiological and behavioral ones, but also psychological well-being. However, when developing comprehensive preventive approaches, psychological intervention tends to receive less attention, despite its possible multiple mechanisms on cardiovascular health. Incorporating both healthy behavior and psychological well-being promotion would be a more efficacious preventive approach. This study aims to investigate the effects of a community-based multicomponent intervention combining positive psychological intervention and lifestyle intervention on improving cardiovascular health among older adults with risk factors of cardiovascular diseases.
Methods
This study is a multicenter, community-based, randomized controlled trial with 18 months of intervention and follow-up for community-dwelling older adults aged 60 to 80 years old with risk factors of cardiovascular health. Intervention activities last six months and are comprised of in-person group training sessions of 60–80 minutes led by trained group instructors and weekly self-monitoring homework. Eligible participants are randomly assigned to a multicomponent intervention “Harmony” group (24 sessions of positive psychology and lifestyle intervention delivered weekly), an active control “Lifestyle” group (eight sessions of lifestyle intervention delivered every 3–4 weeks), or a waitlist control group (no intervention activities). Positive psychological training sessions are designed using well-known techniques derived from positive psychology theories with adaptations to Chinese culture, and lifestyle training sessions are developed according to national guidelines. Primary outcome includes the change of a composite score of systolic blood pressure, total cholesterol, high-density lipoprotein, and low-density lipoprotein levels, as well as psychological well-being measured by Positive Affect and Negative Affect Scale, Meaning in Life Questionnaire, and Satisfaction with Life Scale. Secondary assessments include other measures for physical and biological indicators, psychological well-being, health behaviors, social connection factors, as well as overall cognitive functions.
Discussion
Our results will contribute to providing causal evidence on how psychological well-being combined with health behavior change can be beneficial for cardiovascular health. Given the trial is conducted at the community level, the implementation will provide great potential to benefit a wide range of populations in terms of disease prevention and eventually healthy longevity promotion.
Trial registration:
ChiCTR, ChiCTR2200062929, registered August 24, 2022.
An exploration of vascular cell development in mouse embryoid bodies under hypoxia through single cell RNA-seq
Authors: Acosta-Iborra Bárbara , Puente-Santamaría Laura , Berrouayel Yosra , Peso Luis del , Jiménez Benilde 
Published: February 2024

Abstract
Background: Differentiation of mouse stem cells (mESC) to embryonic bodies (EBs) is a widely used approach to model early stages of development. In this study, we employed single-cell transcriptomics to examine the effect of hypoxia on the differentiation of different cell populations in EBs.
Results: We differentiated EBs for 8 or 10 days, with two time frames of hypoxic or normoxic exposure: a short window of 16h for the 8-day samples, and a 48h window for the 10-day samples, totaling four experimental conditions. The EBs were composed mainly of broad mesodermal progenitors, a limited number of endodermal precursors, and smaller groups of cells that were more advanced in their differentiation pathways. These developed groups included hemoendothelial progenitors, endothelium, cardiomyocytes, and monocyte and erythrocyte precursors. Both O2 and time of differentiation significantly influenced the distribution of cell types across conditions. Our findings revealed that hypoxia induces a pervasive cell cycle arrest signature across all identified cell populations within the EB. Despite the cell cycle arrest, vascular smooth muscle (vSMCs), endothelial, and cardiac cell populations exhibit expansion under hypoxic conditions, while normoxia favours an increase in the monocyte population. Differential proliferation of hemoendothelial progenitors does not appear to be the primary driver of endothelial cells expansion in hypoxia, as their distribution remained similar across treatments and depleted over time. We observed delays in the terminal differentiation pathway of vSMCs precursors in hypoxic conditions, which may contribute to their expansion and migration by switching to a synthetic phenotype. Additionally, oxygen concentration influenced the developmental path of hemangioblasts towards either an endothelial, or myeloid lineage. In the EBs, this was coupled with a shift in presomitic mesoderm differentiation towards endotome or musculoskeletal precursors, which may provide an additional source of progenitors with endothelial potential during oxygen deprivation.
Conclusion: Our study provides novel insights into the cellular responses to hypoxia in the context of early embryonic development, thus unveiling potential mechanisms underlying blood vessel growth.
Confined biofabrication in inflatable bioreactor: toward the sterile production of implantable tissues and organs
Authors: DUFOUR Alexandre , Essayan Lucie , THOMANN Céline , PETIOT Emma , GAY Isabelle , BARBAROUX Magali , MARQUETTE Christophe 
Published: February 2024

Abstract
The future of organ and tissue biofabrication strongly relies on 3D bioprinting technologies. However, maintaining sterility remains a critical issue regardless of the technology used. This challenge becomes even more pronounced when the volume of bioprinted objects approaches organ dimensions. Here, we introduce a novel device called the Flexible Unique Generator Unit (FUGU), which is a unique combination of flexible silicone membranes and solid components made of 3D printed medical-grade polycarbonate. The FUGU is designed to support micro-extrusion needle insertion and removal, internal volume adjustment, and fluid management. The FUGU was tested in various environments, ranging from custom-built basic cartesian to sophisticated 6-axis robotic arm bioprinters, demonstrating its compatibility, flexibility, and universality across different bioprinting platforms. Sterility assays conducted under various infection scenarios highlight the FUGU’s ability to physically protect the internal volume against contaminations, thereby ensuring the integrity of the bioprinted constructs. The FUGU also enabled bioprinting and cultivation of a 14.5 cm³ human colorectal cancer tissue model within a completely confined and sterile environment, while allowing for the exchange of gases with the external environment. This FUGU system represents a significant advancement in 3D bioprinting and biofabrication, paving the path toward the sterile production of implantable tissues and organs.
Comparative metabolomic analysis of the phloem sap of nine citrus relatives with different degrees of susceptibility to Huanglongbing disease.
Authors: HERRANZ MARIA C. , NAVARRO JOSE ANTONIO , LOCASCIO ANTONELLA , PEÑA LEANDRO , MANZANARES PALOMA , MARCOS JOSE F. , PALLAS VICENTE 
Published: February 2024

Abstract
Citrus Huanglongbing (HLB) disease, also known as “citrus greening”, is currently considered the most devastating citrus disease due to its rapid spread, and high severity. Presently, research efforts are focused on searching for either curative treatments or resistant cultivars to combat HLB-associated bacterium ‘Candidatus Liberibacter asiaticus’ (CLas). Metabolomics can help to unravel the mechanisms supporting the potential tolerance/resistance of citrus relatives. Herein, we carried out a metabolomic analysis to determine whether the level of resistance of nine citrus-related genotypes is influenced by their pre-existing metabolic background before infection. For this purpose, the healthy phloem of nine Citrinae genotypes previously categorized according to their different responses to HLB was analyzed. A total of 53 different metabolites were targeted, including amino acids, organic and inorganic acids, and sugars. Interestingly, we observed that resistant and partially resistant genotypes exhibited higher accumulations of organic acids such as quinic acid and citric acid. In contrast, the amount of total sugars showed a clear upward trend in the susceptible genotypes. Notably, within this last group of metabolites, sugar acids displayed a trend toward an average percentage increase in both partially resistant and resistant accessions, being more evident in the resistant group. Changes potentially associated with the level of resistance were observed in certain amino acids within the aspartate and glutamate families. However, only lysine levels were significantly higher in the susceptible samples. The evaluation of five genes associated with lysine catabolism by RT-qPCR revealed differences in transcript abundance between resistant and susceptible samples. These findings open a new avenue of opportunity for identifying metabolites and/or substances that could aid in developing resistance strategies to this devastating disease.
Effect of Particle Shape on the Discharge from a Mono-layer Hopper
Authors: Hanif Muhammad Ahmed , Maza Diego , Meer Devaraj van der 
Published: February 2024

Abstract
In this work, we performed experiments with spheres, rice-shaped particles with different aspect ratios, and macaroni-shaped particles in a quasi two-dimensional hopper, where the thickness was adjusted to the minor dimensions of the particles such that a mono-layered system is created. We quantitatively investigate the vertical velocity and solid fraction profiles at the orifice and determine how these are influenced by the slope of the hopper. Interestingly, where the hopper angle hardly influences the velocity profile for rice-shaped particles, the magnitude of the velocity profile increases for spherical particles and decreases for macaroni particles with the steepness of the hopper. The spheres have flat solid fraction profiles for all hopper angles, but a transition from flat to dome-shaped profiles is observed with decreasing hopper steepness for all non-spherical particles. The discharge rate determined by integrating the product of the velocity and solid fraction profiles has good agreement with the experimentally measured discharge rate for all particle shapes.
Selective anti-tumor activity of Glutathione-responsive abasic site trapping agent in Anaplastic thyroid carcinoma
Authors: Chai Jinyan , Su Mengxue , Zhang Ruiguo , Jia Yuanyuan , Zheng Wei , Tan Jian , Jia Qiang , Sun Huabing , Meng Zhaowei 
Published: February 2024

Abstract
Anaplastic thyroid carcinoma (ATC) is a rare but highly aggressive thyroid cancer with poor prognosis. Killing cancer cells by inducing DNA damage or blockage of DNA repair is a promising strategy for chemotherapy. It is reported that aldehyde-reactive alkoxyamines could capture the AP sites, one of the most common DNA lesions, and inhibit apurinic/apyrimidinic endonuclease 1(APE1)-mediated base excision repair (BER), leading to cell death. Whether this strategy can be employed for ATC treatment is rarely investigated. The aim of this study was to exploit the elevated glutathione (GSH) levels in the tumor micro-environment (TME), and explore the GSH-responsive AP sites capture reagent (AP probe-net), which can be selectively activated by GSH, releasing reactive alkoxyamines to trap AP sites and block the APE1-mediated BER for targeted anti-tumor against ATC. In vitro experiments, including MTT andγ-H2AX assays, demonstrated their selective cytotoxicity. Flow cytometry analysis suggested that AP probe-net arrested the cell cycle in the G2/M phase and induced apoptosis of cells. Western blotting (WB) results showed that the expression of apoptotic protein increased with increasing the concentration of AP probe-net. Further in vivo experiments revealed that the AP probe-net had a good therapeutic effect on subcutaneous tumors of the ATC cells. In conclusion, taking advantage of the elevated GSH in TME, our study affords a new strategy for targeted chemotherapy of ATC with high selectivity and bio-safety.
Multi-Frequency Graph Neural Rough Differential Equations for Traffic Forecasting
Authors: Wang Zengqiang , Zang Di 
Published: February 2024

Abstract
The burgeoning field of neural differential equations (NDEs) underscores a quest for models that not only boast interpretability and robustness but also function within a more generalized framework. Although the fusion of Neural Controlled Differential Equations (NCDEs) with Graph Neural Networks (GNNs) has marked significant strides in traffic forecasting, the prolonged integrative process requisite for long-term forecasts remains a detriment to model efficacy. Furthermore, while NCDEs exhibit prowess in extracting temporal trends within traffic fluctuations, the potential of spatial trend correlations has not been adequately investigated. To bridge this gap, we introduce the Multi-Frequency Graph Neural Rough Differential Equation (MFG-NRDE), an NDE-based model tailored for traffic forecasting. Our model is underpinned by the frequency principle in neural networks and leverages both wavelet and signature transforms. This dual approach not only truncates the integral length but also captures a more nuanced representation of high- and low-frequency temporal features. Complementing this temporal focus, we integrate a novel methodology for dynamically computing spatial correlations, harnessing the inherent trend characteristics of traffic variations to enhance spatial dimension analysis. Empirical validation across four real-world traffic flow datasets demonstrates the superior performance of our MFG-NRDE model, positioning it favorably against contemporary state-of-the-art baselines. This underscores the efficacy of our model in managing the intricate dynamics of traffic data forecasting, paving the way for more sophisticated and accurate predictive models in this domain.
Simulation Study on the Response of Yield and Water Use Efficiency in Spring Wheat to Water Stress at Different Growth Stages Using the APSIM Model
Authors: Wang Jun 
Published: February 2024

Abstract
Water scarcity is the major limiting factor to crop production in the hilly and gully Loess Plateau of central Gansu. A better understanding of the response of crops to the intensity and timing of water stress at different growth stages is helpful for optimizing agricultural production management systems under water-limited conditions. Based on the field experiment at Anding District, Dingxi City, the suitability of the Agricultural Production System Simulator (APSIM) model for simulating spring wheat yield, yield composition, and changes in soil water content under water stress, quantifying the effects of intensity and timing of water stress on yield, water consumption, and water use efficiency (WUE) in spring wheat, and identifying the most sensitive stage of spring wheat to water stress was verified. The results showed that the root mean square error values of the grain numbers, 1000-grain weight, yield, and soil water content of the APSIM model were less than 252.31 number/m2, 1.55 g, 170.26 kg/ha, and 1.73%, respectively. The normalized root mean square error values were less than 6.49, 5.92, 6.03, and 13.78%, respectively. The model effectiveness index values were higher than 0.64, 0.51, 0.52, and 0.50, respectively. The APSIM model had good adaptability to water stress in the study area. Further, the jointing stage was the most sensitive growth period for the water deficit of spring wheat in the study area, and this growth period had the greatest impact on spring wheat yield due to drought. The yield showed a trend of first increasing and then decreasing as irrigation increased. When the irrigation amount reached 300 mm, it reached a maximum value of 4866.19 kg/ha. Notably, WUE achieved a maximum value of 12.32 kg/ha with water stress at the tillering stage, followed by the emergence, jointing, heading, flowering, and grain filling stages. WUE showed a trend of first increasing and then decreasing as irrigation increased. When the irrigation amount reached 300 mm, it reached a maximum value of 15.03 kg/ha. Finally, the variations in yield and WUE with changed water consumption were not synchronous. WUE was at a maximum when irrigation was reduced in regions with severe water shortages, and the yield achieved a maximum with increasing irrigation in regions with relatively abundant water resources. In general, the jointing and grain filling stages were critical times for field irrigation management for spring wheat. Reasonable field irrigation management should be applied at these two growth stages to guarantee a higher yield and WUE of spring wheat in the hilly and gully Loess Plateau of central Gansu.
Determination of Anthracnose (Colletotrichum fructicola) Resistance Mechanism Using Transcriptome Analysis of Resistant and Susceptible Pear (Pyrus pyrifolia)
Authors: Tang Xiaomei , Lu Fen , Xiao Ziwen , Wang Yue , Hu Guoqing , Cai Kexin , Yin Ruichang , Song Wei , Xie Luoluo , Guo Guoling , Wang Wenming , Liu Lun , Liu Li , Ye Zhenfeng , Heng Wei , Guo Xianping , Wang Dongsheng , Jia Bing 
Published: February 2024

Abstract
Background
Anthracnose, mainly caused by Colletotrichum fructicola, leads to severe losses in pear production. However, there is limited information available regarding the molecular response to anthracnose in pears.
Results
In this study, the anthracnose-resistant variety ‘Seli’ and susceptible pear cultivar ‘Cuiguan’ were subjected to transcriptome analysis following C. fructicola inoculation at 6 and 24 h using RNA sequencing. A total of 3186 differentially expressed genes were detected in ‘Seli’ and ‘Cuiguan’ using Illumina sequencing technology. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that the transcriptional response of pears to C. fructicola infection included responses to reactive oxygen species, phytohormone signaling, phenylpropanoid biosynthesis, and secondary metabolite biosynthetic processes. Moreover, the mitogen-activated protein kinase (MAPK) signaling pathway and flavonoid biosynthesis were involved in the defense of ‘Seli’. Furthermore, the gene coexpression network data showed that genes related to plant–pathogen interactions were associated with C. fructicola resistance in ‘Seli’ at an early stage.
Conclusion
Our results showed that the activation of specific genes in MAPK and calcium signaling pathways was highly related to C. fructicola resistance in ‘Seli’ and providing several potential candidate genes for breeding anthracnose-resistant pear varieties.
Obtaining of combined titanium-steel structures by electron beam freeform fabrication using niobium and copper interlayers
Authors: Terentyev Egor V. , Kozyrev Khariton M. , Borodavkina Ksenia T. , Shishkin Dmitriy V. , Sliva Andrey P. , Goncharov Aleksey L. , Gudenko Aleksandr V. , Zhgut Daria A. 
Published: February 2024

Abstract
The work is devoted to the study of bimetallic structures "titanium-steel" by electron beam freeform fabrication with the use of niobium and copper interlayers. A metallographic study of the deposited interlayers is carried out. The hardness distribution over the samples is shown. Technological issues according to deposition of niobium on titanium and steel on copper are pointed out. Tensile testing results reveal that the obtained structures have an ultimate tensile strength of 150–228 MPa and the fracture is located at the niobium-copper alloy side or at the niobium interlayer. The need to reduce the titanium content in niobium due to the occurrence of intergranular penetration of copper is demonstrated.
The species richness of the Salix viminalis rhizosphere at the Stebnyk tailings storages is dependent on supplementation from the Salicornia europaea rhizosphere
Authors: Fetsiukh Anastasiia , Pall Taavi , Timmusk Salme 
Published: February 2024

Abstract
Manipulating the rhizosphere microbiome to enhance plant stress tolerance is an environmentally friendly technology and a renewable resource to restore degraded environments. Here we considered the Salicornia europaearhizosphere community, and the ability of the phytoremediation plant Salix viminalis to recruit its beneficial microbiome to mediate the pollution stress at the Stebnyk mine tailings storage. The tailings contain large amounts of brine salts and heavy metals that contaminate the ground water and surrounding areas, changing soil biogeochemistry and causing increased erosion.
The species richness of the endophytic bacterial community of S. viminalis roots was assessed based on observed OTUs, Shannon- InvSimpson, and evenness index. Our results show that biodiversity was decreased across the contamination zones and that S. europaea supplementation significantly increased the species richness. Our results also indicate that the number of dominating OTUs was not changed across zones in both S. europaea-treated and untreated bacterial populations, and that the decrease in richness was mainly caused by the low abundance of OTUs.
The importance of engineering microbial communities that support the genetic diversity of degraded environments and the challenges with high throughput metabarcoding databases are discussed.