bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic value of mucins (MUC1, MUC2 and MUC5AC) expression profile in endoscopic ultrasound‐guided fine‐needle aspiration specimens of the pancreas
Authors: Wang Yi , Gao Jun , Li Zhaoshen , Jin Zhendong , Gong Yanfang , Man Xiaohua 
Published: August 2007

AbstractMucins are aberrantly expressed in various malignancies. We immunohistochemistrically tested mucins expression (MUC1, MUC2 and MUC5AC) in EUS‐FNA samples from pancreatic occupying lesions for the diagnostic utility. The prevalence of MUC1, MUC2 and MUC5AC expression in pancreatic cancers were 77.5% (31/40), 10.0% (4/40) and 80.0% (32/40), respectively, and in the benign pancreatic diseases 25% (4/16), 31.3% (5/16) and 43.8% (7/16). MUC1 and MUC5AC significantly overexpressed in pancreatic cancer, and MUC1 negatively related with tumor differentiation degree (p < 0.05). The prevalence of MUC1, MUC2 and MUC5AC expression in pancreatic mucinous neoplasms were 66.7% (12/18), 38.9% (7/18) and 88.9% (16/18), respectively, and in the pancreatic nonmucinous neoplasms 60.5% (23/38), 5.3% (2/38) and 57.9% (22/38). MUC2 and MUC5AC significantly overexpressed in pancreatic mucinous neoplasms, especially MUC2 in benign mucinous neoplasms (p < 0.05). Compared with cytology alone, the combination test of MUC1+cytology, and MUC5AC+cytology could achieve higher sensitivity (85 vs. 65%, 100 vs. 65%) and accuracy (89.3% vs. 73.2%, 91.1% vs. 73.2%) for pancreatic cancer diagnosis; the combination test of MUC2 + cytology, and MUC5AC + cytology could achieve higher sensitivity (77.8% vs. 38.9%, 100% vs. 38.9%), and specificity (97.4% vs. 60.5%, 71.1% vs. 60.5%) accuracy (100% vs. 51.8%, 80.4% vs. 51.8%) for mucinous neoplasm diagnosis. The panel MUC1+/MUC2−/MUC5AC+/ was higher specific in pancreatic cancer diagnosis, as well as MUC1−/MUC2+/MUC5AC+/ in pancreatic mucinous neoplasms. Our observations suggest the mucins expression profile in EUS‐FNA specimens has higher value for the diagnosis of pancreatic cancer and mucinous neoplasms. © 2007 Wiley‐Liss, Inc.
99mTc‐MIBI imaging for prediction of therapeutic effects of second‐generation MDR1 inhibitors in malignant brain tumors
Authors: Sasajima Toshio , Shimada Naoya , Naitoh Yuichiro , Takahashi Masataka , Hu Yi , Satoh Tomo , Mizoi Kazuo 
Published: August 2007

AbstractThe aim of this study was to explore whether99mTc‐methoxyisobutylisonitrile (99mTc‐MIBI) is suitable to elucidate multidrug resistance and prediction of potentiation of antitumor agents by second‐generation MDR1 inhibitors (PSC833, MS‐209) in malignant brain tumors in rat. Malignant tumor cells (RG2 and C6 gliomas, Walker 256 carcinoma) were incubated with low dose vincristine (VCR) to induce multidrug resistance. MTT assay demonstrated a significant increase of surviving fractions in VCR‐resistant sublines compared to those of drug‐naive cells. Reverse transcriptase polymerase chain reaction revealed higher expression of MDR1 mRNA in VCR‐resistant cells than drug‐naive cells in each line. Volume distribution (Vd) of99mTc‐MIBI was negatively correlated with MDR1 mRNA expression among drug‐naive and VCR‐resistant cells. MDR1 inhibitors decreased surviving fractions and increasedVdof99mTc‐MIBI significantly in VCR‐resistant sublines, whereas MDR1 mRNA expression was unchanged. These findings indicate that99mTc‐MIBI efflux was functionally suppressed by MDR1 inhibitors. Autoradiographic images of99mTc‐MIBI revealed higher uptake in drug‐naive cells at basal ganglia compared with VCR‐resistant cells at the opposite basal ganglia of rats. Oral administration of the second‐generation MDR1 inhibitors significantly increased99mTc‐MIBI accumulation of both tumors. Therapeutic effects of VCR with or without the MDR1 inhibitors were also evaluated autoradiographically using14C‐methyl‐L‐methionine (14C‐Met) and MIB‐5 index.14C‐Met uptake and MIB‐5 index of both tumors treated with VCR following the MDR1 inhibitor treatment significantly decreased compared with tumors treated with VCR alone. Analysis of99mTc‐MIBI accumulation is considered informative for detecting MDR1‐mediated drug resistance and for monitoring the therapeutic effects of MDR1 inhibitors in malignant brain tumors. © 2007 Wiley‐Liss, Inc.
Revised model core potentials of s‐block elements
Authors: Anjima Hidenori , Tsukamoto Shinya , Mori Hirotoshi , Mine Masaki , Klobukowski Mariusz , Miyoshi Eisaku 
Published: August 2007

AbstractWe developed new model core potentials (MCPs) for s‐block elements from Na to Ra, in which the outer core (n − 1)s and (n − 1)p electrons are treated explicitly together with the ns electrons. By adding suitable correlating functions, we demonstrated that the present MCP basis sets show excellent performance in describing the electronic structures of atoms and molecules, bringing about accurate ionization potentials of atoms and very good spectroscopic constants of ionic and covalent molecules. The results obtained with the new MCPs are very close to the ones obtained using the all‐electron correlation consistent basis sets of Dunning. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Quantitative computer simulations of biomolecules: A snapshot
Authors: Yang Wei , Nymeyer Hugh , Zhou Huan‐Xiang , Berg Bernd , Brüschweiler Rafael 
Published: August 2007

AbstractA recent workshop titled “Quantitative Computational Biophysics” at Florida State University provided an overview of the state of the art in quantitative modeling of biomolecular systems. The presentations covered a wide range of interrelated topics, including the development and validation of force fields, the modeling of protein–protein interactions, the sampling of conformational space, and the assessment of equilibration and statistical errors. Substantial progress in all these areas was reported. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008
Interplay between chromatin remodelers and protein arginine methyltransferases
Authors: Pal Sharmistha , Sif Saïd 
Published: August 2007

AbstractChromatin modifying enzymes have emerged as key regulators of all DNA based processes, which control cell growth, development, and differentiation. Recently, it has become clear that different chromatin remodeling and histone‐modifying activities are involved in transcriptional activation and repression. Among the enzymes involved in regulating chromatin structure is the family of protein arginine methyltransferases (PRMTs) that specializes in methylating both histones as well as key cellular proteins. There are eleven different PRMT genes (PRMT1‐11) whose biological function remains under explored. PRMTs regulate various cellular processes such as DNA repair and transcription, RNA processing, signal transduction, and nucleo‐cytoplasmic localization. Like histone lysine methylation, methylation of histone arginine residues can either induce or inhibit transcription depending on the residue being modified and the type of methylation being introduced. In this review, we will focus on the latest findings and biological roles of ATP‐dependent chromatin remodeling complexes and PRMT enzymes, and how their aberrant expression is linked to cancer. J. Cell. Physiol. 213: 306–315, 2007. © 2007 Wiley‐Liss, Inc.
Tumor suppressor pRb2/p130 gene and its derived product Spa310 spacer domain as perspective candidates for cancer therapy
Authors: Giordano Antonio , Rossi Alessandra , Romano Gaetano , Bagella Luigi 
Published: August 2007

AbstractTumor suppressor pRb2/p130 gene belongs to the retinoblastoma (Rb) gene family, which also includes pRb/p105 and pRb/p107. The members of the Rb gene family have attracted a great deal of interest because of their essential role in regulating cell cycle and, consequently, cell proliferation. This mini review discusses the potential therapeutic applications both of pRb2/p130 and its derived product Spa310 spacer domain in cancer treatment. J. Cell. Physiol. 213: 403–406, 2007. © 2007 Wiley‐Liss, Inc.
Cell surface restriction of EGFR by a tenascin cytotactin‐encoded EGF‐like repeat is preferential for motility‐related signaling
Authors: Iyer Anand Krishnan V. , Tran Kien T. , Griffith Linda , Wells Alan 
Published: August 2007

AbstractThe 14th EGFL‐repeat (Ten14) of human tenascin cytotactin activates the epidermal growth factor receptor (EGFR) with micromolar affinity; however, unlike EGF, Ten14‐mediated activation of EGFR does not lead to receptor internalization. As the divergent signaling pathways downstream of EGFR have been shown to be triggered from plasma membrane and cytosolic locales, we investigated whether Ten14‐mediated surface restriction of EGFR resulted in altered biochemical and cellular responses as compared to EGF. Molecules associated with migratory cascades were activated to a relatively greater extent in response to Ten14, with very weak activation of proliferation‐associated cascades. Activation of phospholipase C γ (PLCγ) and m‐calpain, associated with lamellipod protrusion and tail retraction, respectively, were noted at even at sub‐saturating doses of Ten14. However, activation of ERK/MAPK, p90RSK, and Elk1, factors affecting proliferation, remained low even at high Ten14 concentrations. Similar activation profiles were observed for EGF‐treated cells at 4°C, a maneuver that limits receptor internalization. We demonstrate a concurrent effect of such altered signaling on biophysical responses—sustained migration was observed at levels of Ten14 that activated PLCγ, but did not stimulate proliferation significantly. Here, we present a novel class of EGFR ligands that can potentially signal as a part of the extracellular matrix, triggering specific intracellular signaling cascades leading to a directed cellular response from an otherwise pleiotropic receptor. This work extends the signaling paradigm of EGFL repeat being presented in a restricted fashion as part of the extracellular matrix. J. Cell. Physiol. 214: 504–512, 2008. © 2007 Wiley‐Liss, Inc.
Epidermal growth factor receptor‐dependent PI3K‐activation promotes restitution of wounded human gastric epithelial monolayers
Authors: Tétreault Marie‐Pier , Chailler Pierre , Beaulieu Jean‐Francois , Rivard Nathalie , Ménard Daniel 
Published: August 2007

AbstractRestitution is a crucial event during the healing of superficial injury of the gastric mucosa involving epithelial cell sheet movement into the damaged area. We demonstrated that growth factors promote the restitution of human gastric epithelial cells. However, the intracellular signaling pathways that transmit extracellular cues as well as regulate basal and growth factor‐stimulated gastric epithelial cell migration are still unclear. Herein, confluent human gastric epithelial cell monolayers (HGE‐17) or primary cultures of gastric epithelial cells were wounded with a razor blade and the migration response was analyzed in presence or absence of TGFα or of pharmacological inhibitors of signaling proteins. Kinase activation profile analysis and phase‐contrast microscopy were also performed in parallel. We report that ERK1/2 and Akt activities are rapidly stimulated following wounding of HGE‐17 cells. Treatment of confluent HGE‐17 cells or primary cultures of gastric epithelial cells with the phosphatidylinositol 3‐kinase inhibitor LY294002, but not the MEK1 inhibitor, PD98059, significantly inhibits basal and TGFα‐induced migration following wounding. Conversely, treatment of wounded HGE‐17 cells with phosphatidylinositol(3,4,5)‐triphosphate is sufficient to stimulate basal cell migration by 235%. In addition, pp60c‐src kinase activity and tyrosine phosphorylation of epidermal growth factor receptors (EGFR) are also rapidly enhanced after wounding and pharmacological inhibition of both these activities strongly attenuates basal and TGFα‐induced migration as well as Akt phosphorylation levels. In conclusion, the present results indicate that EGFR‐dependent PI3K activation promotes restitution of wounded human gastric epithelial monolayers. J. Cell. Physiol. 214: 545–557, 2008. © 2007 Wiley‐Liss, Inc.
Ancient linkage of a POU class 6 and an anterior hox‐like gene in cnidaria: implications for the evolution of homeobox genes
Authors: Kamm Kai , Schierwater Bernd 
Published: August 2007

AbstractLinkage analyses in metazoan genomes suggest two ancestral arrays for the majority of homeobox genes. The related homeobox genes and chromosomal regions that are dispersed in extant species derived possibly from only two single common ancestor regions. One proposed ancestral array, designated as ANTP mega‐array, contains most of the ANTP class homeobox genes; the second, named the contraHox super‐paralogon, would consist of the classes PRD, POU, LIM, CUT, prospero, TALE and SIX. Here, we report the tight linkage of a POU class 6 gene to an anterior Hox‐like gene in the hydrozoan Eleutheria dichotoma and discuss its possible significance for the evolution of homeobox genes. POU class 6 genes also seem to be ancestrally linked to the HoxC and A clusters in vertebrates, despite POU homeobox genes belonging to the contraHox paralogon. Hence, the much tighter linkage of a POU class 6 gene to an anterior Hox‐like gene in a cnidarian is possibly the evolutionary echo of an ancestral genomic region from which most metazoan homeobox classes emerged. J. Exp. Zool. (Mol. Dev. Evol.) 308B:777–784, 2007. © 2007 Wiley‐Liss, Inc.
Fatty acid composition of total lipids, neutral lipids and phospholipids in wild and farmed matrinxã (Brycon cephalus) in the Brazilian Amazon area
Authors: Almeida Neiva Maria , Franco Maria Regina Bueno 
Published: August 2007

AbstractBACKGROUND: The chemical composition of Amazonian fish is extremely variable, being influenced by the season and the type and amount of food. A special interest in the fish oil composition has been developed owing to the presence of essential fatty acids, since this is directly related to human health. This study aimed to investigate the fatty acid composition (FAC) of the total lipid (TL), neutral lipid (NL) and phospholipid (PL) fractions of the dorsal muscle and orbital cavity of farmed and wild matrinxã in the Amazon area captured in different seasons.RESULTS: Fatty acids (FA) were analysed by high‐resolution gas chromatography/mass spectrometry. Sixty‐five FA were detected in the TL, 66 in the NL and 55 in the PL. The main FA found in farmed and wild fish were oleic, palmitic, stearic and linoleic acids. No distinctions in the quality or quantity of these fractions between dorsal muscle and orbital cavity were found.CONCLUSION: The season had a significant influence on the TL and FAC. Fish captured during the dry season showed lower levels of lipid and a higher percentage of long‐chain polyunsaturated fatty acids. Matrinxã farmed in a semi‐intensive system showed a nutritional quality comparable to that of wild matrinxã captured in the wet season. Copyright © 2007 Society of Chemical Industry
Effectiveness of organic acid, ozonated water and chlorine dippings on microbial reduction and storage quality of fresh‐cut iceberg lettuce
Authors: Akbas Meltem Yesilcimen , Ölmez Hülya 
Published: August 2007

AbstractBACKGROUND: The comparative effects of organic (citric and lactic) acids, ozone and chlorine on the microbiological population and quality parameters of fresh‐cut lettuce during storage were evaluated.RESULTS: Dipping of lettuce in 100 mg L−1 chlorine solution reduced the numbers of mesophilic and psychrotrophic bacteria and Enterobacteriaceae by 1.7, 2.0 and 1.6 log10 colony‐forming units (CFU) g−1 respectively. Treatment of lettuce with citric (5 g L−1) and lactic (5 mL L−1) acid solutions and ozonated water (4 mg L−1) reduced the populations of mesophilic and psychrotrophic bacteria by 1.7 and 1.5 log10 CFU g−1 respectively. Organic acid dippings resulted in lower mesophilic and psychrotrophic counts than ozonated water and chlorine dippings during 12 days of storage. Lactic acid dipping effectively reduced (by 2.2 log10 CFU g−1) and maintained low populations of Enterobacteriaceae on lettuce for the first 6 days of storage. No significant (P > 0.05) changes were observed in the texture and moisture content of lettuce samples dipped in chlorine, organic acids and ozonated water during storage. Colour, β‐carotene and vitamin C values of fresh‐cut iceberg lettuce did not change significantly (P > 0.05) until day 8.CONCLUSION: Lactic and citric acid and ozonated water dippings could be alternative treatments to chlorine dipping to prolong the shelf life of fresh‐cut iceberg lettuce. Copyright © 2007 Society of Chemical Industry
Low performance of the MSKCC nomogram in preoperatively ultrasonically negative axillary lymph node in breast cancer patients
Authors: Zgajnar Janez , Perhavec Andraz , Hocevar Marko , Podkrajsek Maja , Hertl Kristijana , Frkovic‐Grazio Snjezana , Pohar Maja , Besic Nikola 
Published: August 2007

AbstractBackground and ObjectivesIn order to predict the nonsentinel lymph node (NSLN) metastases in sentinel lymph node (SLN) positive patients a nomogram was created at the Memorial Sloan Kettering Cancer Centre (MSKCC). The aim of our study was to validate the MSKCC nomogram in patients grouped by the preoperative ultrasound (US) examination of the axillary lymph nodes.MethodsThe MSKCC nomogram was validated separately in three groups of patients: (US‐0) only clinically preoperatively negative axillary lymph nodes (126 patients), (US‐1) US negative axillary lymph nodes (109 patients), and (US‐2) US suspicious but fine needle aspiration biopsy (FNAB) negative axillary lymph nodes (41 patients).ResultsThe predicted probability underestimates the actual probability with the mean absolute error equal to 0.116 in the US‐0 group (P = 0.003), and overestimates the actual probability (mean absolute error equal to 0.084) in US‐1 group (P = 0.033) and US‐2 group (mean absolute error is 0.110) (P = 0.275).ConclusionWe found that the MSKCC nomogram overestimates the probability of the NSLN metastases in breast cancer patients with (i) preoperatively US negative or (ii) US suspicious, but FNAB negative axillary lymph nodes. We also found that MSKCC nomogram has only limited value in patients with only clinically negative axillary lymph nodes. J. Surg. Oncol. 2007;96:547–553. © 2007 Wiley‐Liss, Inc.
Glomus tumor of the omentum: A case report
Authors: Matsuo Shigetoshi , Susumu Seiya , Tsutsumi Ryuji , Azuma Takashi , Obata Shirou , Hayashi Tomayoshi 
Published: August 2007

AbstractComputed tomography revealed a well‐enhanced omental mass. Magnetic resonance imaging demonstrated a mass with low signal intensity on T1‐weighted images (WI) and high signal intensity on T2‐WI. Resected specimens immunohistochemically showed positive results for alpha‐smooth muscle actin, muscle‐specific actin (HHF35) and vimentin, and negative results for S‐100 protein, CD34, desmin, EMA, keratin, calretinin, HBME1, and c‐kit. This is the first case of an omental glomus tumor reported in the English literature. J. Surg. Oncol. 2007;96:633–636. © 2007 Wiley‐Liss, Inc.
Safety of modified double‐stapling end‐to‐end gastroduodenostomy in distal subtotal gastrectomy
Authors: Yang Han‐Kwang , Lee Hyuk‐Joon , Ahn Hye‐Sung , Yoo Moon‐Won , Lee In Kyu , Lee Kuhn Uk 
Published: August 2007

AbstractBackground and ObjectivesDouble‐stapling end‐to‐end gastroduodenostomy (DS‐BI) has several potential advantages over other anastomotic techniques in that it is a simple procedure, with no additional gastrotomy on the remnant stomach, and less tension on the anastomosis site. We evaluated the safety of DS‐BI by comparing it with the hand‐sewn Billroth II gastrojejunostomy (B‐II).MethodsMedical records of 933 consecutive patients (DS‐BI 428, B‐II 505) who underwent distal subtotal gastrectomy were retrospectively reviewed. Several clinicopathological features and treatment results were compared between the two groups.ResultsThe overall complication rates were 9.3% in the DS‐BI group and 15.2% in the B‐II group (P = 0.007). Anastomosis‐related complications, such as anastomosis‐site leakage, stenosis, and intraluminal bleeding, did not differ between the two groups (1.2% in the DS‐BI group and 1.8% in the B‐II group, P = 0.59). All the anastomosis‐related complications were managed conservatively. Postoperative mortality rates were 0% in the DS‐BI group and 0.4% (2/505) in the B‐II group.ConclusionsModified DS‐BI is a safe procedure, with short‐term results similar to those of hand‐sewn Billroth II anastomosis. J. Surg. Oncol. 2007;96:624–629. © 2007 Wiley‐Liss, Inc.
Elemental mercury poisoning probably causes cortical myoclonus
Authors: Ragothaman Mona , Kulkarni Girish , Ashraf Valappil V. , Pal Pramod K. , Chickabasavaiah Yasha , Shankar Susarla K. , Govindappa Srikanth S. , Satishchandra Parthasarthy , Muthane Uday B. 
Published: August 2007

AbstractMercury toxicity causes postural tremors, commonly referred to as “mercurial tremors,” and cerebellar dysfunction. A 23‐year woman, 2 years after injecting herself with elemental mercury developed disabling generalized myoclonus and ataxia. Electrophysiological studies confirmed the myoclonus was probably of cortical origin. Her deficits progressed over 2 years and improved after subcutaneous mercury deposits at the injection site were surgically cleared. Myoclonus of cortical origin has never been described in mercury poisoning. It is important to ask patients presenting with jerks about exposure to elemental mercury even if they have a progressive illness, as it is a potentially reversible condition as in our patient. © 2007 Movement Disorder Society
A South African mixed ancestry family with Huntington disease‐like 2: Clinical and genetic features
Authors: Bardien Soraya , Abrahams Fatima , Soodyall Himla , van der Merwe Lize , Greenberg Jacquie , Brink Tinus , Carr Jonathan 
Published: August 2007

AbstractHuntington disease‐like 2 (HDL2) is a neurodegenerative disorder caused by an expansion of a CTG repeat in the junctophilin‐3 gene (JPH3). A limited number of HDL2 families have been reported, all of apparently Black African ancestry. We report on a South African family that presented with progressive dementia and a movement disorder affecting numerous family members. Genotyping of the JPH3 CTG repeat revealed pathogenic expansions in three affected individuals. Whereas HDL2 is thought to be clinically indistinguishable from Huntington disease (HD), 2 of the patients in this study presented with clinical symptoms that differed substantially from HD; one had myoclonus and the other had Parkinsonism. Moreover, brain magnetic resonance imaging scans of these patients showed imaging features atypical for HD. Mitochondrial DNA and Y‐chromosome DNA analysis on a family member showed that his maternal and paternal ancestries are typical of that found among the South African mixed ancestry or colored population. A difference in the distribution of CTG repeats between Caucasian and Black individuals was detected. We conclude that the phenotype of HDL2 is broad and can differ from that of typical HD. The diagnosis therefore should be considered in a wide spectrum of neuropsychiatric and abnormal movement presentations. © 2007 Movement Disorder Society