bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate and prolonged effects of dihydroergocryptine after experimental vascular microsurgery
Authors: Merle Agrügé M. , Restrepo Y. , Benharkate M. , Legeai J. 
Published: March 2007

AbstractAn animal model of limb replantation has been utilized in order to evaluate the effects of dihydroergocryptine (DHEC, 0.1 mg/kg I.V.) on arterial and peripheral tissular blood flows after reperfusion. DHEC is a partial agonist of adrenergic receptors and is therefore liable to exert an action on arteriovenous shunts that remain abnormally opened after ischaemia. DHEC showed important efficacy in this model, increasing, in low doses, blood flow in sutured artery and peripheral tissue.
A digit replantation model
Authors: Haines Peter , Nichter Larry S. , Morgan Raymond F. , Horowitz Jed H. , Edgerton Milton T. 
Published: March 2007

AbstractA simple, inexpensive, reproducible, and reliable model for digital replantation is described utilizing the rabbit ear. When compared to the human digit, it provides benefits over previous models. It is of similar tissue type and characteristics. The vessels closely approximate the digit in size and number. Results are easily quantified and the opportunity for a paired control in the same animal exists. Therefore, experimental design and statistical calculations may be simplified. The model can function for either replantation or revascularization with only slight changes in its preparation. Survival rates have been over 90%. In addition, the model provides an excellent forum for the development of technical expertise in the aspiring microvascular surgeon.
Free vascularized nerve grafts: An experimental study in the rabbit
Authors: Restrepo Yolanda , Merle Michel , Michon Jacques , Folliguet Bernard , Barrat Edouard 
Published: March 2007

AbstractAn experimental animal model of a vascularized graft is presented as an alternative to recover faster function. The technique is described and the model compared to a conventional graft in the sciatic nerve of a rabbit. Results are evaluated with histological studies and quantified using a computer to measure the thickness of the myelin sheath as a correspondence factor of regeneration. Results showed a more favorable regeneration when a vascularized graft was used.
Evaluation of patency of synthetic and autogenous venous and arterial micrografts in rats
Authors: Musella Rosario A. , Willey Edward N. 
Published: March 2007

AbstractLong‐term patency rates of synthetic and autogenous venous and arterial microvascular grafts in rats were compared. The grafts were interposed between the carotid arteries. The geometry of the anastomoses was designed in a manner intended to be comparable to the situation commonly encountered in cerebral revascularization procedures in man. Patency rates were 37.5% for synthetic grafts, 90% for venous grafts, and 60% for arterial grafts. Venous grafts are the best currently available microvascular prostheses.
Relationship of costs and real advantages of microsurgery in urology
Authors: Wagenknecht L. V. 
Published: March 2007

AbstractThe cost‐advantage relationships in the use of microsurgical equipment and techniques in various medical fields are examined. Cost‐effectiveness in clinical urology appears to support the use of microsurgery. Specific applications to urethroplasty, pyeloplasty, renal stone surgery, and renal trauma, are discussed.
An experimental study of the effect of infection on microvascular anastomosis
Authors: Luk K. D. K. , Chow S. P. 
Published: March 2007

AbstractThe effect of penicillin‐resistant Staphylococcus aureus infection on the patency of microvascular anastomosis was studied in an experimental model. Histological and SEM studies were also performed. It was found that the presence of infection did not significantly lower the patency rate of rat femoral artery re‐anastomosis.
Enhancement of blood flow in experimental microvascular free flaps
Authors: Weinberg Hubert , Song Yeliang , Douglas Barry 
Published: March 2007

AbstractProlonged ischemia may result in tissue death because of the no‐reflow phenomenon. By raising a flap 24 hours prior to subjecting it to ischemia, the authors have been able to significantly lengthen the ischemia interval prior to the onset of tissue death. These effects are believed to be the result of alteration of the no‐reflow phenomenon.
A comparative study of autologous peripheral nerve grafts
Authors: Hafiz Mustafa , Lee Sun , Wolf Paul , Haghighi Parviz 
Published: March 2007

AbstractForty‐five Sprague‐Dawley rats were divided into four groups. The first two groups were subjected to primary epineural anastomoses, with and without tension. In the remaining two groups, sciatic nerve defects were produced and femoral cable grafts were then performed with and without making an epineural sheath defect on the edge of the recipient nerves. Five functional evaluation criteria (sensation, push‐off, fanning of claws, cubital ulcer, and leg atrophy) were used along with histological findings. It has been found that the recovery of sensation was the least in the tension group, as was other functional recovery. Animals with epineural and fascicular sutures ranked highest in functional recovery while cable grafts followed at 4th, 10th, and 12th week of observation.
A histologic comparison of continuous and interrupted microarteriorrhaphy
Authors: Joos Karen M. , Blair William F. , Maynard Jerry A. 
Published: March 2007

AbstractInterrupted and continuous microsuturing techniques were compared 3 weeks after microarteriorrhaphy in the rat femoral artery using light microscopy, and internal luminal diameter determinations were measured with a Bio Quant II Digitizer. The histologic appearance of the arteries of both groups was similar, with intimal hyperplasia, intimal incorporation of suture projections into the lumen, minimal inflammatory response to suture material, and restoration of the media. No significant difference was demonstrated between internal diameters at the anastomosis site for the continuous (N = 11, mean diameter 804 ± 60 μm) and interrupted (N = 11, mean diameter 901 ± 45 μm) groups. However, the experimental arteries in both continuous and interrupted groups were significantly dilated when compared to their controls (513 ± 41 μm and 621 ± 44 μm, respectively). This study indicated that interrupted and continuous suture techniques in laboratory microarteriorrhaphy result in similar histologic and morphologic characteristics, and that the repaired vessels were dilated compared to intact vessels.
Fibrinolytic activity following laser‐assisted vascular anastomosis
Authors: Bailes Julian E. , Quigley Matthew R. , Kwaan Hau C. , Cerullo Leonard J. , Brown J. Thomas 
Published: March 2007

AbstractNew technology has allowed tissue bonding to be feasible using carbon dioxide laser energy. Laser‐assisted vascular anastomosis (LAVA) has shown comparable patency results with standard suture anastomosis, but LAVA procedures produce thermally induced transmural structural alterations. To assess functional intimal recovery in LAVA versus sutured vessels, a fibrinolytic slide technique was utilized in 21 rats which had LAVA performed in one femoral artery while the opposite limb underwent a conventional microsuture anastomosis. Conventional anastomosis had persistent fibrinolytic activity while LAVA had return of fibrinolytic activity by 48 hours. Intimal fibrinolysis inhibition following LAVA is reversible and this technique does not lead to an increased incidence of thrombosis.
Vascularization of tricalcium phosphate, an artificial bone substitute: Preliminary observations
Authors: Angel Michael F. , Swartz William M. , Ramasastry Sai S. , Brown Mary Lynn , Hanley Edward N. , Herbert David L. 
Published: March 2007

AbstractTricalcium phosphate (TCP), a biodegradable artificial bone substitute, was used to study the following questions: (i) can TCP be vascularized; (ii) do ectopically placed implants have osteoinductive capacity; and (iii) does vascularization affect implant resorption?Implants were placed in dogs in several areas: (i) subcutaneous controls; (ii) wrapped with latissimus dorsi muscle; (iii) wrapped in omentum; (iv) saphenous and thoracodorsal av pedicles threaded through implant. Prior to harvest, animals were given tetracycline and implants were harvested at 3, 6, and 9 weeks. Microfil was injected prior to sacrifice and specimens were xeroradiographed. Histology and computerized axial tomography were obtained. From this study, we conclude that (1) TCP can be vascularized; (2) the most effective method of vascularization was by an AV pedicle threaded through the implant; (3) vascularized implants did not have osteoinductive properties per se, and (4) in the time observed no difference in implant resorption was demonstrated between the test groups.
Experimental study of lymphatic contractility in lymphedema and its clinical significance
Authors: Wang Guo‐Ying , Zhong Shi‐Zhen 
Published: March 2007

AbstractFifty‐four rats that had been used as models for lymphedema were divided into nine groups to observe the changes of lymphatic contractility in pathologic conditions at varying postoperative days. The changes of the lymphatic contractility were divided into three stages in this paper: In the first stage, the lymphatic contractility was impaired by high intralymphatic pressure, but the morphology of its wall was normal and it could recover its function if the pressure was decreased. In the second stage, the blocked lymphatic lost its contractile function partially or completely because its wall thickened and fibrosed, but the newborn lymphatics possessed contraction function and could be anastomosed. In the third stage, most lymphatics had lost their contractile function because of severe fibrosis. The fibrosis of the tissue even blocked the growth of the newborn lymphatics. In this stage, therefore, it was unsuitable to do a lymphatic‐venous anastomosis. It can be concluded from the experiment that lymphatic contractility should be considered before performing a lymphatic‐venous anastomosis. The lymphatic that still has contractile function should be selected for lymphatic‐venous anastomosis.
A model of experimental lymphedema in rats' limbs
Authors: Wang Guo‐Ying , Zhong Shi‐Zhen 
Published: March 2007

AbstractThe authors' modified Olszewski method (J Cardiovasc Surg 9:178–183, 1968) for preparing chronic lymphedema with rats' limbs is presented. The incidence of chronic lymphedema formation after 9 months of follow‐up was 64%. The experiment demonstrates that there is a brisk regeneration of the lymphatics under the lymphedema. The regeneration observed took place on the lateral wall of the blocked lymphatics, except for regeneration of capillary lymphatics, and the newborn lymphatics grew about 1 mm each day. There are indeed preexisting lymphatics in the body. They are of significant importance in the establishment of the collateral circulation because of their great number and early opening. Lymphatic regeneration and opening could be prevented by tissue scarring. Effective prevention of the formation of tissue scarring has important significance in preventing the development of chronic lymphedema.
Microsurgical application of freeze‐dried venous allografts
Authors: Pratt Michael F. , Schneider James , Norris Michael S. 
Published: March 2007

AbstractFreeze‐dried venous interposition allografts with an internal diameter of 1.0 mm and a length of 1.0 cm were placed into the femoral arteries of 17 Sprague‐Dawley albino male rats in order to investigate patency and host tissue response. The immediate patency rate was 88%. The epigastric island flap was monitored as a sign of patency in the early postoperative period (defined as 1 to 3 days). Twelve of the 17 subjects were observed for 2 months, at which point 66% (8/12) remained patent. There were two aneurysmal dilatations. Histopathological studies and transmission electron microscopy demonstrated that freeze‐dried veins undergo complete remodeling in vivo by a normal reparative process and that they do not induce a cellular immune response in the host.
Rat epigastric pedicle model: A clinically relevant evaluation of 1‐mm ptfe grafts
Authors: Barttelbort Scott W. , O'leary Michael J. , Schneider James , Woodruff Woody 
Published: March 2007

AbstractTwenty‐eight Sprague‐Dawley rats had 1.0‐mm polytetrafluoroethylene (PTFE) interposition grafts placed in their femoral artery that supplied a 3.0 × 3.0 cm epigastric pedicle flap. The model is the first to evaluate 1.0‐mm PTFE under rigorous, clinically simulating conditions. Anastomoses employed a new, continuous telescoping suture technique. Grafts were selected for histological and electron microscopic evaluation, which confirmed the development of a cellular neointimal lining. Viability of the flap model had a 90% correlation with graft patency. Overall graft patency was 50%. Patency was documented in some specimens harvested at 200 days. The flap model is a good indicator of graft patency. PTFE grafts of 1.0‐mm internal diameter are not yet equivalent to autologous veins. Future refinements, particularly aseptic technique, are expected to result in even higher patency rates.
Experience with epigastric free flap allograft in the rat
Authors: Ruby Leonard K. , Greene Mark , Risitano Giovanni 
Published: March 2007

AbstractWe report our continuing experience with the rat epigastric free vascularized flap as a transplant model. Four groups of ten pure‐bred rats underwent transplantation of epigastric vessel based skin flaps. The flaps were immediately revascularized in the recipient animal and the results recorded. No immunologic manipulation was performed. Rejection and acceptance of the flaps occurred according to known genetic predictions in these 40 procedures. This study will serve as a control for immunologic manipulation experiments.
Ischemic induction of neovascularization: A study by fluorometric analysis
Authors: Semashko Denise , Song Yeliang , Silverman David G. , Weinberg Hubert 
Published: March 2007

AbstractThe dynamic process of skin flap neovascularization can be quantitated in a relatively non‐invasive fashion in an „in‐vivo”︁ model using the technique of perfusion fluorometry. The extent of skin flap ischemia can be monitored, and its effect on angiogenesis rapidly determined. Evidence is presented that indicates that angiogenesis develops from a distal to proximal direction corresponding to the degree of ischemia as measured by fluorescein uptake and elimination. Furthermore, although certain proximal segments of skin flaps demonstrated no uptake or elimination, survival could still occur, indicating that neovascularization need not be complete at the time of pedicle interruption.
Extraintracranial anastomosis performed by means of biological gluing materials: Experimental and clinical study
Authors: Aksik I. A. , Kikut R. P. , Apshkalne D. L. 
Published: March 2007

AbstractA new surgical technique of extraintracranial anastomosis (EIA) is reported. End‐to‐side microvascular anastomosis is performed by applying four crossed‐fixing sutures and by mantling a hemostatic sponge muff (eg, Spongostan) impregnated with fibrinogen‐thrombin glue. Histopathological studies of microvascular anastomosis in animals and clinical results of 70 EIA operations showed that this surgical technique of anastomosis has certain advantages, namely that it reduces the number of sutures needed, is less traumatic to the vascular wall, ensures elastic vascular junction, and the glue and Spongostan used possess high biological inertness that provides a better physiological connection of vessels than conventional suturing. The highly hermetic vascular junction and absence of blood flow at the anastomosis site are achieved without additional suturing. The duration of the operation is significantly reduced. A full EIA function sets in comparatively early.
The inner prosthetic surface structure and re‐endothelization: An experimental study in the rat using two types of microvascular prostheses for aortic implantation
Authors: Hess F. , Jerusalem C. , Braun B. , Grande P. 
Published: March 2007

AbstractTwo types of microvascular prostheses were implanted in the rat infrarenal aorta. Operations were carried out with clean, nonsterile instruments under ether anesthesia. Anastomoses were made with a continuous 8‐0 suture. In group A, a 1‐cm‐long piece of expanded polytetrafluorethylene (PTFE) and in group B a 1‐cm‐long fibrous polyurethane prosthesis were implanted. Both groups consisted of 18 rats. Three rats from each group were killed at days 3, 5, 10, 20, 40, and 60 postimplantation. Prostheses were examined by scanning electron and light microscopy for the re‐endothelialization. All prostheses in both groups were patent at the time of death. Reendothelialization started in both types of prostheses the fifth day after implantation and had advanced 1–3 mm in the PTFE prostheses at day 60. However, in the fibrous polyurethane prostheses, re‐endothelialization progressed and a complete new lining was achieved between days 20 and 40 postimplantation. The endothelium/neointima in the fibrous prosthesis was firmly anchored onto the prosthetic wall by means of cellular protrusions between the polyurethane fibers. In contrast to this observation, the endothelium/neointima developed in the PTFE prostheses was not anchored to the wall of the prosthesis. It is emphasized that the development of a new lining in a prosthesis may reduce the risk of endogenous, hematogenous infections. From the results of this study, we have concluded that there is a correlation between the inner surface structure and the extent of the reendothelialization of a prosthesis. A prosthesis with a fibrous structure is much more rapidly and completely re‐endothelialized than an expanded PTFE prosthesis.
Simultaneous reconstruction of bone and skin defects by free fibular graft with a skin flap
Authors: Minami Akio , Usui Masamichi , Ogino Toshihiko , Minami Michio 
Published: March 2007

AbstractIt has been very difficult to accomplish simultaneous reconstruction of a massive defect of the long bone associated with a large skin defect. Yoshimura et al reported a new procedure using a vascularized free fibular transplant with a monitoring flap. This procedure was used in four cases that had resulted in massive bone and skin defects after wide resection of benign and malignant bone tumors occurring in the long bones. Our results using the composite vascularized fibular graft were gratifying.
A new microsurgical reoperation for failed lumbar disc surgery
Authors: Goald Harold J. 
Published: March 2007

AbstractThis paper reports a consecutive series of 103 patients operated upon over a 4.2‐year period (from November 1978 through January 1983). These patients were initially operated upon by other orthopedic and neurologic surgeons without relief of their sciatic pain. The average duration of symptoms prior to microsurgical reoperation was 35.9 months. The average number of reoperations per patient was 1.4. The median follow‐up time was 27 months (range 1–50 months). Preoperative evaluation included examination, CAT scan, myelogram, and electromyeogram. Average postoperative hospital stay without spinal fusion was 5.1 days. Two techniques were used: a unilateral decompression and a bilateral intersegmental decompression. Results are compared. A classification of operative findings is given. Eight patients in the series received spinal fusion as part of their procedure. Microsurgical reoperation benefited 80.6% of the 103 patients.
Waveform analysis applied to laser doppler flowmetry
Authors: Fischer Joseph C. , Parker M. Paul , Shaw William W. 
Published: March 2007

AbstractLaser Doppler flowmetry (LDF) has been found useful in monitoring free flaps. In the present study, waveform analysis has been applied to the LDF waveform. Waveform changes were investigated in the microcirculation during varying degrees of arterial or venous occlusion in the saphenous island and flap of the dog and the human digit. The quantitative analysis of laser Doppler flowmetry waveform allows for the diagnosis of arterial occlusion or venous occlusion and their differentiation.
Spiral interrupted suturing technique for microvascular anastomosis: A comparative study
Authors: Chen Lilly , Chiu David T. W. 
Published: March 2007

AbstractThis is an experimental study comparing the suturing time and patency rate of a spiral interrupted suturing technique to those of conventional interrupted and continuous suturing technique in end‐to‐end as well as end‐to‐side anastomosis. The spiral interrupted suturing technique requires less time for either end‐to‐end or end‐to‐side anastomosis than conventional interrupted suturing technique, and does not result in stenosis as shown in the venous end‐to‐end anastomosis by continuous suturing technique.
Experimental laser‐assisted end‐to‐side microvascular anastomosis
Authors: Sartorius Carl J. , Shapiro Scott A. , Campbell Robert L. , Klatte Eugene C. , Clark Steven A. 
Published: March 2007

AbstractThe authors present a new technique of end‐to‐side microvascular anastomosis in a rat carotid artery model, employing a milliwatt CO2 laser. Both carotid arteries were isolated and approximated in an end‐to‐side fashion by the placement of four 10‐0 nylon stay sutures. The milliwatt CO2 laser was used to effect vessel anastomosis between the sutures, using 70–100 mW of power. Animals were killed 8 weeks postoperatively. Angiography of each anastomosis was performed in all animals. All anastomoses were then harvested, and submitted for histological analysis. Anastomotic patency was 100%, both intraoperatively and angiographically. There was no evidence of intravascular thrombus, anastomotic stenosis, or pseudoaneurysm formation. Early in the experiment, some anastomoses showed localized dilatation at the anastomotic site. The histologic changes at the anastomotic site are described. Laser‐assisted microvascular anastomosis is a feasible technique, and a potential alternative to conventional suture techniques.
Hemodynamics after autogenous, interpositional grafting in small arteries
Authors: Blair William F. , Chang Laurette , Pedersen Douglas R. , Gabel Ronald H. , Bell Lawrence D. 
Published: March 2007

AbstractThis investigation initiates the quantitative, hemodynamic assessment of interpositional grafts in small rat arteries. The left femoral arteries in 16 Sprague‐Dawley rats were transected at two levels, and were then repaired using interrupted suturing technique. This effectively provided an ideally sized and histologically matched interposition graft. The 20‐MHz PUDVM method was used to measure blood velocities, and derive values for vessel lumen area, temporal mean of the spatial mean velocity (Vsm), and volumetric flows. Measurements were completed distal to the interposed graft. Variables were quantitated in the preoperative and at the 5‐, 15‐, and 30‐minute postoperative intervals. Statistical analysis of data indicated that the interpositional grafting procedure resulted in increased vessel lumen area and decreased Vsm, but, importantly, volumetric flow (about 8.20 ml/min) remained unchanged. This study demonstrated that, although hemodynamic characteristics are altered, volumetric flow can be restored after an experimental, interpositional grafting procedure in small arteries.