bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of metabolism-related subtypes and feature genes in Crohn’s disease
Authors: Liu Xizhi , Gu Shanzhi , Zhao Xinhan , Zhang Yujiao 
Published: March 2024

Abstract
Crohn's disease is a chronic inflammatory disorder of the gastrointestinal tract, capable of causing disruptions in gastrointestinal function. However, the precise etiology of the disease remains unclear at present. This study aims to analyses metabolism-related signatures to identify feature genes. To investigate potential treatment targets to improve patient prognosis. We downloaded Crohn's disease (CD) datasets from the GEO database, we identified three subtypes (MCA, MCB, MCC) through consensus cluster, as well as the correlation with immune infiltration. We selected the MCA cluster for weighted gene correlation network analysis (WGCNA). After selecting the strongly correlated red module, we identified 20 hub genes. We conducted KEGG, GO functional enrichment analyses. Further analysis using LASSO, SVM and protein-protein interaction (PPI) methods narrowed down to 11 hub genes. Taking the intersection with differentially expressed genes, we finally obtained 4 feature genes: ACTN1, MMP2, THY1, and ZCCHC24. We constructed a nomogram and used the DGIdb database for candidate drug prediction. Molecular docking validation was performed using Schrödinger software to calculate and visualize the interactions between MMP2 and 19 drugs. Through the analysis of metabolism-related signatures, we have identified 4 feature genes and used them to construct a nomogram. This model is expected to offer novel insights for clinical treatment.
SA and NHP glucosyltransferase UGT76B1 affects plant defense in both SID2- and NPR1-dependent and independent manner
Authors: Zhang Wei , Maksym Rafał , Georgii Elisabeth , Geist Birgit , Schäffner Anton 
Published: March 2024

Abstract

The small-molecule glucosyltransferase UGT76B1 inactivates salicylic acid (SA), isoleucic acid (ILA), and N-hydroxypipecolic acid (NHP). ugt76b1 loss-of-function plants manifest an enhanced defense status. Thus, we were interested how UGT76B1 genetically integrates in defense pathways and whether all impacts depend on SA and NHP. We study the integration of UGT76B1 by transcriptome analyses of ugt76b1. The comparison of transcripts altered by the loss of UGT76B1 with public transcriptome data reveals both SA-responsive, ISOCHORISMATE SYNTHASE 1/SALICYLIC ACID INDUCTION DEFICIENT 2 (ICS1/SID2)/NON EXPRESSOR OF PR GENES 1 (NPR1)-dependent, consistent with the role of UGT76B1 in glucosylating SA, and SA-non-responsive, SID2/NPR1-independent genes. We also discovered that UGT76B1 impacts on a group of genes showing non-SA-responsiveness and regulation by infections independent from SID2/NPR1. Resistance of ugt76b1 against Pseudomonas syringae is partially independent from SID2 and NPR1. In contrast, the ugt76b1-activated resistance is completely dependent on NHP (FLAVIN-DEPENDENT MONOOXYGENASE 1, FMO1). Moreover, FMO1 ranks top among the ugt76b1-induced SID2- and NPR1-independent pathogen responsive genes, suggesting that FMO1 determines the SID2- and NPR1-independent effect of ugt76b1. Furthermore, the genetic study revealed that FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), SID2, and NPR1 are required for the SA-JA crosstalk and senescence development of ugt76b1, indicating that EDS1 and FMO1 have a similar effect like stress-induced SA biosynthesis (SID2) or the key SA signaling regulator NPR1. Thus, UGT76B1 influences both SID2/NPR1-dependent and independent plant immunity, and the SID2/NPR1 independence is relying on FMO1 and its product NHP, another substrate of UGT76B1.
May the Pathogenic Microbes Not Be with You: Core Microbiome Profiling in Hospital Airspace
Authors: Hussin Husna Mat , Tay Darren Dean , Zainulabid Ummu Afeera , Maghpor Mohd Norhafsam , Ahmad Hajar Fauzan 
Published: March 2024

Abstract

The hospital indoor air microbiome, a diverse range of microorganisms, gains prominence amid the COVID-19 pandemic. Elevated awareness underscores implications for patient and staff well-being. Concerns about risks to indoor air quality persist due to prolonged indoor exposure, necessitating further research on specific threats within the hospital environment. In this study, an independent culture-based approach was used to analyze the baseline core microbiome present in hospital environments, utilizing amplicon sequencing on the next-generation sequencing technology to target the V3 region of the 16S rRNA gene. Firmicutes, Proteobacteria, and Actinobacteria were the main bacterial phyla that were most isolated from the wards and clinics with different orders of abundance; Firmicutes being associated more in clinics and Actinobacteriota in wards. The bacteria Niallia taxi, Methyloversatilis universalis, unclassified Rummeliibacillus, unclassified Clostridium, and unclassified Sphingomonadaceae dominated the clinic area while ward areas reported Pseudonocardia bannensis, Rubrobacter A aplysinae, unclassified Brachybacterium, unclassified Bradyrhizobium, and unclassified Mycobacterium to be the top five features. While the alpha-diversity index showed no significant differences, the beta-diversity analysis showed a significant differences between clinic and ward areas (p < 0.05). Certain bacterial species associated with opportunistic pathogens as well as normal skin flora such as Methylobacterium spp., Cutibacterium spp., unclassified Sphingomonadaceae, and Anoxybacillus B spp., were also identified across all samples. The methods described in this research aim to establish a rapid and sensitive screening process that could be valuable for disease surveillance within the healthcare setting, shedding light on the potential impacts of the hospital microbiome on human illness.
Trends and predictors of changes in renal function after radical nephrecto my for renal tumors
Authors: Yan Yongchao , Wang Xinning , Liu Yunbo , Li Bin , Xu Shang , Du Haotian , Li Yanjiang 
Published: March 2024

Abstract
Background: Chronic kidney disease (CKD) is a common postoperative complication in patients who undergo radical nephrectomy for renal tumours. However, the factors influencing long-term renal function require further investigation.
Objective: This study aimed to investigate the trends in renal function changes and risk factors for renal function deterioration in patients with renal tumours after radical nephrectomy.
Results: We analysed the data from 329 patients with renal tumours undergoing radical nephrectomies between January 2013 and December 2018. We monitored the changes in the patient’s renal function before and after surgery for 3 years. The progression of renal function was determined by the progression and degradation of CKD stages. Univariate and multivariate logistic regression analyses were used to analyse the causes of renal function progression. In this study, 43.7% of patients had postoperative acute kidney injury (AKI) and 48.3% had upgraded CKD stages. Further research revealed that the patient’s renal function levelled off 3 months following surgery. Additionally, renal function changes during these 3 months have a substantial impact on the progression of long-term renal function in patients.
Conclusion: AKI may be an indicator of short-term postoperative changes in renal function. Renal function tests should be performed in patients with AKI after radical nephrectomy to monitor the progression of functional impairment, particularly within the first 3 months after radical nephrectomy.
Refugee Children and Peer- Relationships; Evaluations of Teachers
Authors: YILMAZ AYŞE DURAN 
Published: March 2024

Abstract

Background
Refugee children need to make new friends in the countries where they live, adapt, and get to know each other with other children. This study aims to examine the peer relationships of refugee children in the preschool period. For this purpose, the peer relationships of 160 refugee children were analyzed.
Method
The method used in this study is mixed, and the design of the study is an explanatory sequential mixed design. The research participants were 160 refugee children who took preschool education in Gaziantep in the 2023–2024 academic year. The qualitative data needed to examine refugee children's classroom peer relationships in more detail were provided by five preschool teachers.
Results
According to the results obtained from the study, the gender of the children was found to be an effective factor in peer relations, aggression, and social skills. The Turkish-speaking level of refugee children positively affected their peer relations, social skills, and total scores, it did not have a significant effect on the aggression dimension. There was no significant correlation found between the class sizes of the children and their peer relations, aggression, social skills, and total scores. As a result of the interviews with the teachers, they stated that the social behavior of refugee children in the classroom varies according to their knowledge of Turkish, adaptation to Turkish culture, financial status, and gender of the child.
A mixed method impact assessment of the use of aerial logistics to improve maternal health and emergencies outcomes in the Ashanti Region of Ghana
Authors: Kremer Pedro , Haruna Florence , Briegleb Christina , Amoah Maame Esi , Oteng Kenneth Fosu , Boadi Stephanie , Dwomoh Samuel , Bentil Adwoa , Tinkorang Emmanuel 
Published: March 2024

Abstract

Maternal mortality, a critical global public health issue, is notably higher in low-to-middle-income countries (LMICs). In Ghana, access to healthcare, especially maternal and emergency services, is hindered by infrastructural deficiencies such as poor road networks and unreliable electricity, making the delivery of medical supplies and services difficult. The study used a mixed-method approach to assess the impact of aerial logistics on various maternal health outcomes in the Ashanti Region, focusing on the intervention by Zipline Mpanya distribution center. The study was conducted in the Ashanti Region of Ghana, where clinical outcome data were sourced from the health directorate. Pre-exposure years were 2017–2020, and post-exposure years were 2021–2022. Statistical analysis involved univariate explorations, T-tests, ANOVAs, and Zero-Inflated Poisson regressions, adjusting for factors like poverty. The qualitative study was to describe and analyze changes in providers’ and patients’ satisfaction, perceived quality across maternal health and health emergencies, and experiences using or being treated with the aerial logistics. The Zero-Inflated Poisson regression analysis indicated significant pre-post increases in antenatal visits and deliveries by 19.9% and 25% respectively, and a notable 3.4-fold increase in referrals, while emergency visits decreased by 4.4%. Maternal deaths reduced significantly by 56.4%, with no substantial changes observed in rates of hysterectomies and neonatal deaths. Patients and healthcare providers perceived improvement in access to maternal and emergency medications. In conclusion, this study underscores the groundbreaking role of medical drones in enhancing public health strategies, especially in LMICs, by significantly improving maternal health outcomes.
Validation of the Arabic version of the Mukbang Addiction Scale
Authors: Saeed Wizra , Merdad Nisma , Amin Rizwana , Rashid Tabassum , Hallit Souheil , Fekih-Romdhane Feten 
Published: March 2024

Abstract
Introduction:
The present study evaluated the psychometric properties of the Arabic translation of the Mukbang Addiction Scale (MAS) among Arabic-speaking adults from the general population. Specifically, it aimed to assess the factorial structure through a confirmatory factor analysis, determine the composite reliability through Cronbach alpha and McDonald's omega scores, assess gender invariance, and evaluate the convergent validity by examining its correlation to eating addiction and psychological distress.
Methods
A total of 370 individuals with a mean age of 21.94 ± 2.29 years participated in this study, which was conducted using an online platform. The participants were surveyed on demographic information, mukbang addiction, food addiction, and psychological distress. Translation was conducted using the forward and backward technique.
Results
The findings demonstrated excellent internal consistency of the Arabic MAS (McDonald's omega coefficient = .93). Confirmatory factor analyses validated the one-factor structure of the scale, while establishing measurement invariance across sex at the configural, metric, and scalar levels. No sex differences were observed in the Mukbang addiction levels. Lastly, the MAS scores were significantly and positively correlated with food addiction and psychological distress, supporting their convergent validity.
Conclusion
The current research provides evidence supporting the reliability and validity of the Arabic version of the MAS as a self-report method for assessing addictive Mukbang watching. While further validations are needed to corroborate the present findings, this measure can be effectively utilized across different fields, including schools, mental health centers, and researchers aiming to understand this global phenomenon.
Integration of Hybrid Renewable Energy Systems for Hydrogen Refueling Stations
Authors: Mohammed Awsan , Alabbas Abbas , Ghaithan Ahmed M. , Darghouth Mohamed Noomane , Attia Ahmed M. 
Published: March 2024

Abstract

This study investigates hydrogen production using renewable energy, with an emphasis on system reliability and hydrogen storage methods for periods of insufficient electric power. The study was conducted at a refueling station in Dumat Al-Jandal, Saudi Arabia. It utilized HOMER software to simulate different arrangements incorporating solar (PV) arrays, wind turbines (WT), converters, batteries, electrolyzers, and hydrogen tanks. The purpose is to optimize the system to fulfill a daily hydrogen need of 25 kg. The results indicate that the most efficient setup consists of a 208 kW photovoltaic (PV) array, 7 wind turbine (WT) units, a 71 kW converter, a 100 kW electrolyzer, a 100 kg hydrogen storage tank, and a 342-battery array. The arrangement attains a levelized energy cost of $0.452 per kilowatt-hour, a hydrogen production cost of $6.22 per kilogram, and a net present cost of $645,000, allowing a significant reduction of CO2 emissions by preventing the release of 372 metric tons of CO2 per year. The findings provide stakeholders with crucial information on optimizing hybrid renewable energy for hydrogen production at refueling stations. The study underscores the significant potential for integrating such systems into refueling infrastructures as a pathway toward environmentally sustainable and economically viable hydrogen production.
Negative association between Low-dose blood lead levels and bone mineral density in U.S. children: a cross-sectional study from NHANES 2005–2010
Authors: 吴 徐金 , Jin XIxin , Xu Wei , She Chang , LI Liubin , Mao Yongtao 
Published: March 2024

Abstract

Background
Relationship between lead exposure and children's bone health remains controversial. The aim of this study was to investigate the relationship between blood lead levels (BLL) and femur bone mineral density (BMD). All data came from the National Health and Nutrition Examination Survey (NHANES) database, 2005–2010.
Methods
A total of 3331 subjects aged 8–18 years were enrolled in this study. Multivariate linear regression models were used to explore the relationship between BLL and femur BMD. And further stratified analyses by gender and ethnicity. Additionally smooth curve fitting was used to explore the saturation threshold effect between the two.
Result
There was a significant negative correlation between BLL and BMD in different parts of the femur in children. For every 1ug/dl increase in BLL, total femur BMD, femoral neck BMD, femoral trochanter BMD, and femoral intertrochanteric BMD decreased by 0.008 g/cm2, 0.007 g/cm2, 0.007 g/cm2, and 0.008 g/cm2, and the saturation threshold for BLL were 1.73ug/dl; 1.61ug/dl, 1.80 ug/dl, and 1.66ug/dl.
Conclusion
This study shows that low-dose blood lead levels, even those within the reference range, can have a significant negative impact on children's bone health.
Association between maternal antidepressant use during pregnancy and the risk of autism spectrum disorder and attention deficit hyperactivity disorder in offspring
Authors: Lee Min-Jing , Chen Yi-Lung , Wu Shu-I , Huang Chien-Wei , Dewey Michael E , Chen Vincent Chin-Hung 
Published: March 2024

Abstract
Prenatal antidepressant exposure has been reported to be associated with adverse neurodevelopmental outcomes, yet studies considering confounding factors in Asian populations are lacking. This study utilized a nationwide data base in Taiwan, enrolling all liveborn children registered in the National Health Insurance system between 2004 and 2016. Subjects were divided into two groups: antidepressant-exposed (n=55,707)) and antidepressant-unexposed group (n=2,245,689). The effect of antidepressant exposure during different trimesters on autism spectrum disorder (ASD) and attention-deficit/hyperactivity disorder (ADHD) was examined. Sibling controls and parallel comparisons by paternal exposure status were treated as negative controls. Additional sensitivity analyses were conducted to examine the effects of antidepressant exposure before and after pregnancy. Prenatal antidepressant exposure was associated with increased risks of ASD and ADHD in population-wide and adjusted analysis. However when comparing antidepressant-exposed children with their unexposed siblings, no differences were found for ASD (Hazard ratio [HR]: 1.04, 95% confidence interval [CI]: 0.76-1.42 in first trimester; HR: 0.96, 95% CI: 0.62-1.50 in second trimester; HR: 0.69, 95% CI: 0.32-1.48 in third trimester) and ADHD (HR: 0.98, 95%CI: 0.84-1.15 in first trimester; HR: 0.91, 95% CI: 0.73-1.14 in second trimester; HR: 0.79, 95% CI: 0.54-1.16 in third trimester) . Increased risks for ASD and ADHD were also noted in paternal control, before and after pregnancy analyses. These results imply that the association between prenatal antidepressant exposure and ASD and ADHD is not contributed to from an intrauterine medication effect but more likely to be accounted for by maternal depression, genetic, and potential environmental factors.
LI-RADS for Diagnosing Hepatocellular Carcinoma by Contrast-enhanced US with SonoVue and Sonazoid—A Single Center Prospective Study
Authors: Zhai Hong-yan , Yang Xiao-huan , Lin Chen , Liu Quan-yan , Zhou Gui-ming , Shan Chang-liang , Zhu Xin-yuan 
Published: March 2024

Abstract

Purpose: To compare the diagnostic performance of two algorithms for HCC diagnosis: SonoVue-CEUS based on CEUS LI-RADS version 2017 and a modified algorithm incorporating Kupffer-phase findings for Sonazoid-CEUS.
Materials and Methods: This single center prospective study enrolled high-risk patients for HCC. Each participant underwent same-day SonoVue-CEUS and Sonazoid-CEUS. Each liver observation was assigned two LI-RADS categories according to each algorithm: SonoVue-CEUS LI-RADS SHF and modified Sonazoid-CEUS LI-RADS. For the latter method, observations at least 10 mm with nonrim arterial phase hyperenhancement were upgraded LR-4 to LR-5 if there was no washout with a Kupffer defect and were reassigned LR-M to LR-5 if there was early washout with mild Kupffer defect. The reference standard was pathologic confirmation.
Results: Overall, 66 patients (mean age, 61.2 years ± 10.9; 54 male patients, 12 female patients) with 66 observations (mean size, 31mm ± 16) were eventually enrolled. Compared with the SonoVue-CEUS LI-RADS, the results of Sonazoid-CEUS LI-RADS showed significant changes in sensitivity (88% vs. 63%, P＜0.001), accuracy (85% vs. 68%, P＜0.001). There was no significant difference in specificity (71% vs 76%, P =.26).
Conclusion: When incorporating Kupffer-phase findings, Sonazoid-CEUS LI-RADS had higher sensitivity without loss of specificity comparing with SonoVue-CEUS LI-RADS.
Background: Liver Imaging Reporting and Data System (LI-RADS) was designed for contrast-enhanced US (CEUS) with pure blood pool agents for diagnosis of hepatocellular carcinoma (HCC), such as SonoVue, but Kupffer-cell agents, such as Sonazoid, allow additional lesion characterization in the Kupffer phase yet remain unaddressed.
Preparation and Performance of Highly Sensitive and Selective Mercury Fluorescence Sensor Based on Rhodamine Tetraphenylporphyrin
Authors: Li Ling , Yao Yinuo , Wang Xiaowan , Yu Yaoqi , Ji Tailong , Li Han , Li Yi , Liu Yuanyuan 
Published: March 2024

Abstract
A highly selective fluorescent probe Por-RBO for Hg2+ was designed and synthesized with Rhodamine B and porphyrin as the fluorophore. The sensor can determine the Hg2+ of a solution within the pH 5.0 to 8.0, free from interference of other metal ions. When detected in buffer solution, the fluorescence was the strongest when 5 times the concentration of Hg2+ ion was added. Calculated by fluorescence titration method, the detection limit of probe Por-RBO for Hg2+ ion was as low as 0.12µmol/L. The geometries of Por-RBO and Por-RBO- Hg2+ were optimized at the B3LYP/6-31G** level by density functional theory. The charge distribution, orbital interactions and bonding characteristics were analyzed and compared in detail to discuss the recognition mechanism and structure-fluorescence property relationships. The results of cell fluorescence imaging and CCK-8 experiments showed that the probe Por-RBO has low cytotoxicity and can be used for fluorescence distribution imaging of Hg2+in living cells.
Aphicidal and antifeedant activity of Synandrospadix vermitoxicus extracts against Myzus persicae on potato plants
Authors: López-Isasmendi Guadalupe , Machado-Assefh Cristina Renata , Reyes María Guadalupe , Uriburu María Laura , Alvarez Adriana Elisabet 
Published: March 2024

Abstract

Potato crops are usually kept virus-free through intensive aphid vector control schemes that require multiple treatments with insecticides. The generalist aphid Myzus persicae developed different mechanisms of resistance to insecticides, and there is a need for sources of novel insecticides. Synandrospadix vermitoxicus (Griseb.) Engl., an Araceae family native to the northwestern region of Argentina, Paraguay, and Bolivia has been locally described as having insecticidal properties against insect larvae and could be a potential source of new natural insecticides against aphids. We tested the antifeedant and aphicidal effects of two extracts from the tubers of S. vermitoxicus, ethanolic and hexanic, on M. persicae. First, we treated potato leaves with the extracts to assess their antifeedant effect by measuring host preference changes in M. persicae. Then, we evaluated its aphicidal effect by offering the extracts to aphids through artificial diets and the aphid probing behaviour by electrical penetration graph. We also analysed the extracts for the main classes of secondary metabolites. We found that both extracts have antifeedant effects, with the hexanic being the strongest and accordingly, aphid probing behaviour was affected on leaves treated with hexanic extract. While the ethanolic extract affected the survival of aphids fed on artificial diets, the hexanic extract did not. The analysis of S. vermitoxicus extracts shows an array of flavonoids and triterpenoids compounds. Therefore, our results show that the tubers of this plant could be a source for a novel product with potential use on the control of M. persicae on potato crops.