bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of factors associated with postoperative infection following sacral tumor resection
Authors: Sciubba Daniel M. , Nelson Clarke , Gok Beryl , McGirt Matthew J. , McLoughlin Gregory S. , Noggle Joseph C. , Wolinsky Jean Paul , Witham Timothy F. , Bydon Ali , Gokaslan Ziya L. 
Published: November 2008

Object
Resection of sacral tumors has been shown to improve survival, since the oncological prognosis is commonly correlated with the extent of local tumor control. However, extensive soft-tissue resection in close proximity to the rectum may predispose patients to wound complications and infection. To identify potential risk factors, a review of clinical outcomes for sacral tumor resections over the past 5 years at a single institution was completed, paying special attention to procedure-related complications.

Methods
Between 2002 and 2007, 46 patients with sacral tumors were treated with surgery. Demographic data, details of surgery, type of tumor, and patient characteristics associated with surgical site infections (SSIs) were collected; these data included presence of the following variables: diabetes, obesity, smoking, steroid use, previous surgery, previous radiation, cerebrospinal fluid leak, number of spinal levels exposed, instrumentation, number of surgeons scrubbed in to the procedure, serum albumin level, and combined anterior-posterior approach. Logistic regression analysis was implemented to find an association of such variables with the presence of SSI.

Results
A total of 46 patients were treated for sacral tumor resections; 20 were male (43%) and 26 were female (57%), with an average age of 46 years (range 11–83 years). Histopathological findings included the following: chordoma in 19 (41%), ependymoma in 5 (11%), rectal adenocarcinoma in 5 (11%), giant cell tumor in 4 (9%), and other in 13 (28%). There were 18 cases of wound infection (39%), and 2 cases of repeat surgery for tumor recurrence (1 chordoma and 1 giant cell tumor). Factors associated with increased likelihood of infection included previous lumbosacral surgery (p = 0.0184; odds ratio [OR] 7.955) and number of surgeons scrubbed in to the operation (p = 0.0332; OR 4.018). Increasing age (p = 0.0864; OR 1.031), presence of complex soft-tissue reconstruction (p = 0.118; OR 3.789), and bowel and bladder dysfunction (p = 0.119; OR 2.667) demonstrated a trend toward increased risk of SSI.

Conclusions
Patients undergoing sacral tumor surgery may be at greater risk for developing wound complications due to the extensive soft-tissue resections often required, especially with the increased potential for contamination from the neighboring rectum. In this study, it appears that previous lumbosacral surgery, number of surgeons scrubbed in, patient age, bowel and bladder dysfunction, and complex tissue reconstruction may predict those patients more prone to developing postoperative SSIs.
Calibration laws based on multiple linear regression applied to matrix‐assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
Authors: Williams Jr. D. Keith , Chadwick M. Ashley , Williams Taufika Islam , Muddiman David C. 
Published: November 2008

AbstractOperation of any mass spectrometer requires implementation of mass calibration laws to translate experimentally measured physical quantities into a m/z range. While internal calibration in Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR‐MS) offers several attractive features, including exposure of calibrant and analyte ions to identical experimental conditions (e.g. space charge), external calibration affords simpler pulse sequences and higher throughput. The automatic gain control method used in hybrid linear trap quadrupole (LTQ) FT‐ICR‐MS to consistently obtain the same ion population is not readily amenable to matrix‐assisted laser desorption/ionization (MALDI) FT‐ICR‐MS, due to the heterogeneous nature and poor spot‐to‐spot reproducibility of MALDI. This can be compensated for by taking external calibration laws into account that consider magnetic and electric fields, as well as relative and total ion abundances. Herein, an evaluation of external mass calibration laws applied to MALDI‐FT‐ICR‐MS is performed to achieve higher mass measurement accuracy (MMA). Copyright © 2008 John Wiley & Sons, Ltd.
Romantic and sexual activities, parent–adolescent stress, and depressive symptoms among early adolescent girls
Authors: Davila Joanne , Stroud Catherine B. , Starr Lisa R. , Miller Melissa Ramsay , Yoneda Athena , Hershenberg Rachel 
Published: November 2008

AbstractBuilding on evidence that romantic experiences are associated with depressive symptoms in adolescence, we examined their bidirectional association, as well as the role of sexual activity and parent–adolescent stress in their association. Data were collected from 71 early adolescent girls (M age 13.45 years; SD = 0.68) and their primary caregiver initially and one year later. Results indicated that adolescents who engaged in more romantic activities experienced increases in depressive symptoms over time. Second, greater depressive symptoms predicted romantic involvement and sexual activities, including intercourse, one year later. Third, dysphoric adolescents who were experiencing higher parent–adolescent stress were the most likely to engage in subsequent sexual intercourse. Implications for understanding how the association between depressive symptoms and romantic and sexual experiences develops and the course of this association are discussed.
Psychosocial and psychiatric factors associated with adolescent suicide: A case–control psychological autopsy study
Authors: Portzky Gwendolyn , Audenaert Kurt , van Heeringen Kees 
Published: November 2008

AbstractThis study aimed at the investigation of psychosocial and psychiatric risk factors of adolescent suicide by means of a case–control psychological autopsy study.Relatives and other informants of 19 suicide victims and 19 matched psychiatric controls were interviewed by means of a semi‐structured interview schedule. Psychiatric controls included adolescents, individually matched according to gender, age and time between interview and suicide/admission, who had been admitted to a Psychiatric Department with suicidal ideation or attempted suicide at admission.Results showed that suicide victims had been exposed more frequently to suicidal behaviour by friends and through media and experienced more relational problems in the past year. Suicidal communication was less frequently reported in suicide victims than in controls and when communication did occur, it was less often directed towards parents. Treatment of psychiatric disorders was significantly less found in suicide victims. Psychiatric control patients were more likely to have comorbidity of psychiatric disorder, conduct disorder, delinquency or academic difficulties.This study showed significant differences between young suicide victims and psychiatric controls for life events, exposure, communication and treatment. These results also suggest that more symptoms and more externalizing behaviour can be observed in psychiatric control patients which could indicate more warning signals of possible psychiatric problems for the environment, which could result in more help‐seeking behaviour and treatment.
Effect of genistein‐8‐C‐glucoside from Lupinus luteus on DNA damage assessed using the comet assay in vitro
Authors: Rucinska Agata , Gabryelak Teresa 
Published: November 2008

AbstractGenistein‐8‐C‐glucoside (G8CG) belongs to natural isoflavones phytoestrogens, which are a subclass of flavonoids, a large group of polyphenolic compounds widely distributed in plants, with possible anticarcinogenic effects in various in vitro systems and in vivo animal models. We used glycosylated genistein (genistein‐8‐C‐glucoside) from flowers of lupine (Lupinus luteus L.) to study its cytotoxic and genotoxic effects on mouse embryonic fibroblast (line NIH 3T3). The MTT assay to assess cytotoxicity and comet assay for the detection of DNA damage were used. The cells were exposed to various concentrations of genistein‐8‐C‐glucoside (2.5–110 μM) and hydrogen peroxide (5–90 μM). The effect of G8CG alone or in combination with H2O2 was determined. G8CG at concentrations >20 μM significantly reduced cell viability and induced DNA damage. In contrast, lower concentrations of (2.5–10 μM) G8CG showed antioxidant properties against H2O2‐induced DNA damage with no associated toxicity.
The efficient use of enterprise information for strategic advantage: A data envelopment analysis
Authors: Bendoly Elliot , Rosenzweig Eve D. , Stratman Jeff K. 
Published: November 2008

AbstractA majority of manufacturers make use of some form of enterprise systems (ES), yet on average, the financial impact of ES adoption is essentially neutral. We propose that in an ES environment of easy information access, competitive success depends, in part, on the policies regulating enterprise information use. To explore this proposition, we examine the efficient use of different types of enterprise information in the realization of strategic performance. Efficient firms will devote fewer resources to information use to achieve the same strategic performance as less efficient firms.We employ data envelopment analysis (DEA) using data collected from Enterprise Resource Planning (ERP) system adopters at two different points in time in order to calculate a measure of efficient information use. This information efficiency metric is validated as a strong predictor of Compustat profitability. Additional analyses suggest that the most efficient users of information tend to emphasize information related to operational excellence. Regardless of information emphasis, however, efficient manufacturers – in contrast to their less efficient counterparts – were more likely to exhibit a better match between the most emphasized type of information and the corresponding dimension of strategic performance.
Quantification of Ureaplasma urealyticum DNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels
Authors: JACOBSSON BO , AALTONEN RIIKKA , RANTAKOKKO‐JALAVA KAISU , MORKEN NILS‐HALVDAN , ALANEN ANNA 
Published: November 2008

AbstractObjective. To study the effect of the amniotic fluid quantity of Ureaplasma urealyticum DNA on inflammatory response levels in women with preterm labor (PTL) and preterm prelabor rupture of membranes (pPROM). Design. A prospective multi‐center follow up study. Setting. Sahlgrenska University Hospital, Göteborg, Sweden and Turku University Hospital, Turku, Finland. Sample. Eleven U. urealyticum positive samples obtained after transabdominal amniocenteses in 197 women presenting with PTL and pPROM. Methods. The U. urealyticum positive samples were analyzed with real‐time polymerase chain reaction, using the Lightcycler instrument with primers specific for U. urealyticum 16 S rDNA. The amniotic fluid samples were analyzed for tumor necrosis factor (TNF)‐α, interleukin (IL)‐6, IL‐1β and IL‐10 with enzyme‐linked immunosorbent assays. Main outcome measures. Correlation between U. urealyticum DNA concentrations in the amniotic fluid and inflammatory cytokine levels. Results. The concentrations of U. urealyticum DNA varied between 0.024 and 934 μg/mL. A significant correlation between U. urealyticum DNA and TNF‐α level was observed. No correlation with the other cytokines was found. Women with PTLhad higher levels of U. urealyticum DNA and a different cytokine pattern than women with pPROM. Conclusions. U. urealyticum in the amniotic fluid induces an inflammatory reaction in a dose dependent manner and the quantity of U. urealyticum DNA is well correlated with the level of the inflammatory cytokine TNF‐α.
Prognostic factors influencing the development of an iatrogenic pneumothorax for computed tomography-guided radiofrequency ablation of upper renal tumor
Authors: Park B. K. , Kim C. K. 
Published: November 2008

Background: Percutaneous radiofrequency (RF) ablation of upper renal tumors is considered a minimally invasive treatment, but this technique may cause pneumothorax. Purpose: To assess retrospectively the prognostic factors influencing the development of iatrogenic pneumothorax for RF ablation of upper renal tumors. Material and Methods: Computed tomography (CT)-guided RF ablation was performed in 24 patients (21 men, three women; age range 31–77 years, mean age 53.3 years) with 28 upper renal tumors. Various factors for pneumothorax-complicated (PC) upper renal tumors and non-pneumothoracic (NP) upper renal tumors were compared during RF ablation to determine which of the factors were involved in the development of pneumothorax. Results: Among 28 upper renal tumors in 24 patients, a pneumothorax occurred accidentally in six patients with eight tumors and intentionally in two patients with two tumors. This complication was treated with conservative management, instead of tube drainage. PC upper renal tumors had shorter distance from the lung or from the costophrenic line to the tumor, a larger angle between the costophrenic line and the tumor, and a higher incidence of intervening lung tissue than NP upper renal tumors ( P<0.01). Intervening lung tissue was more frequently detected on CT images obtained with the patient in the prone position than on CT images obtained with the patient in the supine position. Conclusion: The presence of intervening lung tissue and the close proximity between an upper renal tumor and the lung are high risk factors for developing an iatrogenic pneumothorax. Pre-ablation CT scan should be performed in the prone position to exactly evaluate intervening lung tissue.
Proton magnetic resonance spectroscopic findings of cerebral fat embolism induced by triolein emulsion in cats
Authors: Baik S K , Kim Y-W , Kim H J , Lee J W , Cho B. M. , Kim D-H , Choi S H , Lee S H , Chang K H 
Published: November 2008

Background: In experimental studies, embolization of the cerebral hemisphere with triolein emulsion has revealed reversible magnetic resonance imaging (MRI) findings in the subacute stage. Purpose: To investigate the changes in the major metabolites, by proton magnetic resonance spectroscopy (MRS), in a cerebral fat embolism induced by a triolein emulsion. Material and Methods: The internal carotid arteries of 19 cats were injected with a triolein emulsion, and multivoxel MRS was performed 30 min, 1 day, and 7 days later. In the control group, six cats were injected with normal saline. The MR spectra were evaluated for N-acetyl aspartate (NAA), creatine (Cr), and choline (Cho), along with the presence of lipid and lactate. Semiquantitative analyses of NAA/Cr, Cho/Cr, NAA/Cho, and lipid/Cr ratios compared the median values of the ipsilateral metabolite ratios with those of the contralateral side and in the control group for each point in time. Results: The NAA/Cr, Cho/Cr, and NAA/Cho ratios in the ipsilateral cerebral hemisphere of the embolized group after 30 min, 1 day, and 7days were not significantly different from the contralateral hemisphere of the embolized and control groups ( P>0.05). The lipid/Cr ratio in the ipsilateral cerebral hemisphere of the embolized group was significantly higher when compared with the control group ( P=0.012 at 30 min, P=0.001 on day 1, and P=0.018 on day 7). Conclusion: Cerebral fat embolism induced by a triolein emulsion resulted in no significant change in the major metabolites of the brain in the acute stage, except for an elevated lipid/Cr ratio, which suggests the absence of any significant hypoxic-ischemic changes in the lesions embolized using a fat emulsion.
Delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) of cadaveric shoulders: Comparison of contrast dynamics in hyaline and fibrous cartilage after intraarticular gadolinium injection
Authors: Wiener E. , Hodler J. , Pfirrmann C. W. A. 
Published: November 2008

Background: Delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) is a novel method to investigate cartilaginous and fibrocartilaginous structures. Purpose: To investigate the contrast dynamics in hyaline and fibrous cartilage of the glenohumeral joint after intraarticular injection of gadopentetate dimeglumine. Material and Methods: Transverse T1 maps were acquired on a 1.5T scanner before and after intraarticular injection of 2.0 mmol/l gadopentetate dimeglumine in five cadaveric shoulders using a dual flip angle three-dimensional gradient echo (3D-GRE) sequence. The acquisition time for the T1 maps was 5 min 5 s for the whole shoulder. Measurements were repeated every 15 min over 2.5 hours. Regions of interest (ROIs) covering the glenoid cartilage and the labrum were drawn to assess the temporal evolution of the relaxation parameters. Results: T1 of unenhanced hyaline cartilage of the glenoid was 568±34 ms. T1 of unenhanced fibrous cartilage of the labrum was 552±38 ms. Significant differences ( P=0.002 and 0.03) in the relaxation parameters were already measurable after 15 min. After 2 to 2.5 hours, hyaline and fibrous cartilage still demonstrated decreasing relaxation parameters, with a larger range of the T1(Gd) values in fibrous cartilage. T1 and ▵R1 values of hyaline and fibrous cartilage after 2.5 hours were 351±16 ms and 1.1±0.09 s−1, and 332±31 ms and 1.2±0.1 s−1, respectively. Conclusion: A significant decrease in T1(Gd) was found 15 min after intraarticular contrast injection. Contrast accumulation was faster in hyaline than in fibrous cartilage. After 2.5 hours, contrast accumulation showed a higher rate of decrease in hyaline cartilage, but neither hyaline nor fibrous cartilage had reached equilibrium.
Antioxidant and Vasodilatory Effects of Heme Oxygenase on Mesenteric Vasoreactivity Following Chronic Hypoxia
Authors: SWEAZEA KAREN , WALKER BENJIMEN R. 
Published: November 2008

ABSTRACTObjective:Chronic hypoxia (CH) results in impaired vasoconstriction associated with increased expression of heme oxygenase (HO). We hypothesized that enhanced HO activity minimizes reactive oxygen species (ROS) in arteries from CH rats, thereby normalizing endothelium‐dependent vasodilation and concurrently produces carbon monoxide (CO), resulting in tonic vasodilation.Methods:ROS were quantified in mesenteric arteries from control and CH Sprague‐Dawley rats. Reactivity to the endothelium‐dependent vasodilator, acetylcholine (ACh), and the vasoconstrictor, phenylephrine (PE), were also assessed.Results:Basal ROS levels did not differ between groups and were similarly increased by HO inhibition. In contrast, catalase inhibition increased ROS in CH rats only. Vasodilatory responses to ACh were not different between groups. Combined inhibition of catalase and HO impaired PE‐induced vasoconstriction in both groups. CH‐induced impairment of vasoconstriction was reversed by either catalase or HO inhibition supporting the protective roles of the HO and catalase pathways following CH. Increased vascular smooth muscle calcium was observed with inhibition in the CH group, suggesting that catalase and HO‐derived CO elicit reduced calcium influx, leading to the impaired vasoconstriction.Conclusions:Our data suggest that although the HO pathway is an important antioxidant influence, impaired vasoconstriction following CH appears to be due to effects of ROS and HO‐derived CO.
Thermal Facilitation of Lymphocyte Trafficking Involves Temporal Induction of Intravascular ICAM‐1
Authors: CHEN QING , APPENHEIMER MICHELLE M. , MUHITCH JASON B. , FISHER DANIEL T. , CLANCY KRISTEN A. , MIECZNIKOWSKI JEFFERY C. , WANG WAN‐CHAO , EVANS SHARON S. 
Published: November 2008

ABSTRACTObjective: Fever is associated with improved survival, although its beneficial mechanisms are poorly understood. Previous studies indicate that the thermal element of fever augments lymphocyte migration across high endothelial venules (HEVs) of lymphoid organs by increasing the intravascular display of a gatekeeper trafficking molecule, intercellular adhesion molecule‐1 (ICAM‐1). Here, we evaluated the spatio‐temporal relationship between the thermal induction of intravascular ICAM‐1 and lymphocyte trafficking.Methods: Intravascular ICAM‐1 density was quantified by immunofluorescence staining in mice exposed to fever‐range whole‐body hyperthermia (39.5±0.5°C). ICAM‐1–dependent lymphocyte trafficking was measured in short‐term homing assays.Results: A linear relationship was observed between the duration of heat treatment and intravascular ICAM‐1 density in HEVs with maximal responses requiring sustained (i.e., five hours) thermal stress. Circulating lymphocytes were found to sense incremental changes in ICAM‐1 on HEVs, such that trafficking is proportional to the intravascular density of ICAM‐1. We further identified a hydroxamate‐sensitive shedding mechanism that restores ICAM‐1 expression to homeostatic levels following the cessation of thermal stress.Conclusions: The time‐dependent response to thermal stress indicates that ICAM‐1 density governs the efficiency of lymphocyte interactions with HEVs in vivo. These studies highlight the dynamic role of the microcirculation in promoting immune surveillance during febrile inflammatory responses.
The Cauliflower Mosaic Virus Protein P6 Forms Motile Inclusions That Traffic along Actin Microfilaments and Stabilize Microtubules
Authors: Harries Phillip A. , Palanichelvam Karuppaiah , Yu Weichang , Schoelz James E. , Nelson Richard S. 
Published: November 2008

AbstractThe gene VI product (P6) of Cauliflower mosaic virus (CaMV) is a multifunctional protein known to be a major component of cytoplasmic inclusion bodies formed during CaMV infection. Although these inclusions are known to contain virions and are thought to be sites of translation from the CaMV 35S polycistronic RNA intermediate, the precise role of these bodies in the CaMV infection cycle remains unclear. Here, we examine the functionality and intracellular location of a fusion between P6 and GFP (P6-GFP). We initially show that the ability of P6-GFP to transactivate translation is comparable to unmodified P6. Consequently, our work has direct application for the large body of literature in which P6 has been expressed ectopically and its functions characterized. We subsequently found that P6-GFP forms highly motile cytoplasmic inclusion bodies and revealed through fluorescence colocalization studies that these P6-GFP bodies associate with the actin/endoplasmic reticulum network as well as microtubules. We demonstrate that while P6-GFP inclusions traffic along microfilaments, those associated with microtubules appear stationary. Additionally, inhibitor studies reveal that the intracellular movement of P6-GFP inclusions is sensitive to the actin inhibitor, latrunculin B, which also inhibits the formation of local lesions by CaMV in Nicotiana edwardsonii leaves. The motility of P6 along microfilaments represents an entirely new property for this protein, and these results imply a role for P6 in intracellular and cell-to-cell movement of CaMV.