bluuebunny/crossref_metadata_2025_split huggingface dataset:
C6orf15 promotes liver metastasis via WNT/β-catenin signalling in colorectal cancer
Authors: Yu Jiankang , Sun Jian , Tang Jingtong , Xu Jiayu , Qian Guanru , Zhou Jianping 
Published: April 2024

Abstract
Background
Colon cancer ranks third among global tumours and second in cancer-related mortality, prompting an urgent need to explore new therapeutic targets. C6orf15 is a novel gene that has been reported only in Sjogren’s syndrome and systemic lupus erythematosus patients. We found a close correlation between increased C6orf15 expression and the occurrence of colon cancer. The aim of this study was to explore the potential of C6orf15 as a therapeutic target for colorectal cancer.

Method
RNA-seq differential expression analysis of the TCGA database was performed using the R package ‘limma.’ The correlation between target genes and survival as well as tumour analysis was analysed using GEPIA. Western blot and PCR were used to assess C6orf15 expression in colorectal cancer tissue samples. Immunofluorescence and immunohistochemistry were used to assess C6orf15 subcellular localization and tissue expression. The role of C6orf15 in liver metastasis progression was investigated via a mouse spleen infection liver metastasis model. The association of C6orf15 with signalling pathways was assessed using the GSEA-Hallmark database. Immunohistochemistry (IHC), qPCR and western blotting were performed to assess the expression of related mRNAs or proteins. Biological characteristics were evaluated through cell migration assays, MTT assays, and Seahorse XF96 analysis to monitor fatty acid metabolism.

Results
C6orf15 was significantly associated with liver metastasis and survival in CRC patients as determined by the bioinformatic analysis and further verified by immunohistochemistry (IHC), qPCR and western blot results. The upregulation of C6orf15 expression in CRC cells can promote the nuclear translocation of β-catenin and cause an increase in downstream transcription. This leads to changes in the epithelial–mesenchymal transition (EMT) and alterations in fatty acid metabolism, which together promote liver metastasis of CRC.

Conclusion
Our study identified C6orf15 as a marker of liver metastasis in CRC. C6orf15 can activate the WNT/β-catenin signalling pathway to promote EMT and fatty acid metabolism in CRC.
Construction and preliminary validation of a tool to measure the needs of adolescents and young adults (AYA) diagnosed with cancer: the QUestionnaire nEEd Cancer AYAs: QUEEC-AYAs
Authors: Justafré S. , Marino P. , Touzani R. , Dupeyre-Alvarez F. , Dantin P. , Viens P. , Vey N. , Calvin S. 
Published: April 2024

Abstract
Context
In France, 2300 adolescents and young adults (AYAs, 15–25 years old) are diagnosed with cancer each year. As soon as the disease is diagnosed, a number of physical, psychological and social needs may arise. The aim of this study is to develop a tool to measure unmet needs that will allow the specificities of AYAs to be understood while allowing health care staff to mobilise the necessary actors to resolve them.

Methods
We developed the Questionnaire nEEd Cancer AYAs (QUEEC-AYAs questionnaire), from two existing questionnaires: the Cancer Needs Questionnaire Young People and the Needs Assessment & Service Bridge. A main sample of 103 AYAs then received and completed the questionnaire in order to conduct an exploratory factor analysis.

Results
The final structure of the QUEEC-AYAs is composed of 7 dimensions and 48 items: information (8 items), cancer care team (6 items), Physical health (4 items), Emotional health (14 items), Sexual & reproductive health (6 items), Health behaviors & wellness (4 items), Daily life (6 items). The questionnaire has a good acceptability and all domains have a Cronbach’s alphas value above 0.80.

Conclusion
The QUEEC-AYAs is the first measure of the psychosocial needs of AYAs available in French. Its systematic use in health care services should improve the coordination of care required by AYAs during and after treatment.

Trial registration
This study was approved by the ethics committee of the Paoli-Calmettes Institute (IRB # IPC 2021-041, 2021 May 20).
Biomimetic peroxidase MOF-Fe promotes bone defect repair by inhibiting TfR2 and activating the BMP2 pathway
Authors: Xue Yaxin , Xu Wei , Zhao Danyang , Du Zijing , Jiang Hao , Lv Hao , Zhang Dong , Yu Zhencheng , Cao Yi , Han Dong 
Published: April 2024

Abstract
Background
Large bone defects pose a clinical treatment challenge; inhibiting transferrin receptor 2 (TfR2), which is involved in iron metabolism, can promote osteogenesis. Iron-based metal-organic frameworks (MOF-Fe) particles not only inhibit TfR2 but also serve as biomimetic catalysts to remove hydrogen peroxide in reactive oxygen species (ROS); excess ROS can disrupt the normal functions of osteoblasts, thereby hindering bone regeneration. This study explored the potential effects of MOF-Fe in increasing osteogenic activity and clearing ROS.

Methods
In vitro experiments were performed to investigate the osteogenic effects of MOF-Fe particles and assess their impact on cellular ROS levels. To further validate the role of MOF-Fe in promoting bone defect repair, we injected MOF-Fe suspensions into the femoral defects of SD rats and implanted MOF-Fe-containing hydrogel scaffolds in rabbit cranial defect models and observed their effects on bone healing.

Results
In vitro, the presence of MOF-Fe significantly increased the expression levels of osteogenesis-related genes and proteins compared to those in the control group. Additionally, compared to those in the untreated control group, the cells treated with MOF-Fe exhibited a significantly increased ability to remove hydrogen peroxide from ROS and generate oxygen and water within the physiological pH range. In vivo experiments further confirmed the positive effect of MOF-Fe in promoting bone defect repair.

Conclusion
This study supports the application of MOF-Fe as an agent for bone regeneration, particularly for mitigating ROS and activating the bone morphogenetic protein (BMP) pathway, demonstrating its potential value.
The associations of fatty acids related dietary patterns with overweight and obesity among Chinese children
Authors: Huang Zhi , Ma Keyu , Yin Xiaochen , Li Ziming , Chen Ming , Duan Yujie , Li Li , Hu Yuming 
Published: April 2024

Abstract
Background
Childhood overweight and obesity is becoming an emerging face of malnutrition. The aims of this study were to develop fatty acid (FAs) related dietary patterns and explored the associations of FAs related dietary patterns with overweight and obesity among Chinese children.

Methods
An observational study was conducted on 435 children aged 4 to 7 years old in South Central China. Erythrocyte FAs composition was analyzed by gas chromatography-mass spectrometry. Diet was collected by food frequency questionnaires and dietary patterns were evaluated by reduced rank regression. The logistic regression analysis was used to exploring the association of dietary patterns with overweight and obesity.

Results
The prevalence of overweight, obesity, and overweight or obesity were 6.52, 4.59, and 11.11% in Chinese children, respectively. Twenty five types of FAs were detected in erythrocyte of children and four FAs related dietary patterns were identified. The dietary pattern positively correlated with n-3 PUFAs, but negatively with SFAs,was characterized by high intake of fish, shrimp, crab and shellfish, leaf-off vegetable, nuts, and tubers, which have a significantly decreased overweight risk (OR = 0.580, 95%CI: 0.375 ∼ 0.895, P = 0.014).The pattern positively strong associated with n-6 PUFAs, but negatively strong with n-3 PUFAs, had high intake of snacks, leaf-off vegetable, fresh beans, and coarse cereals, which have a significantly decreased obesity risk (OR = 0.518, 95%CI: 0.325 ∼ 0.827, P = 0.006).

Conclusion
Four FAs related dietary patterns were identified. The dietary pattern with high intake of fish, shrimp, crab and shellfish decreased overweight risk by increasing n-3 PUFAs, and decreasing SFAs. The dietary pattern with high intake of plant food, decreased obesity risk by providing an balanced n-6/n-3 PUFAs ratio.
Landing Page via Perceived Interactivity for Four Generational Cohorts
Authors: Thomas Mary Rani , Mathew Jain 
Published: April 2024

Purpose- There are numerous online websites for buying products and services today. The challenge for marketers is to adopt the pull strategy, which attracts customers to the website and creates additional sales. This strategy can materialise only when innovation is brought in. A landing page on the web is considered to be the initial entry point for creating innovation. The current research paper emphasises the importance of creating a landing page for bringing innovation that caters to the four generations.
Design/method /approach-400 responses were obtained equally from male and female respondents of each generational category except the alpha generation. Data was collected through a survey using Google forms. The structural equation model was used for analysis, and generational age was considered the moderator.
Findings-There is a gradual shift in the generational pattern of perceiving and understanding technology to buy from the website. Being tech-savvy, in the case of generation Z the impact of perceived interactivity in creating purchase intention is very strong, followed by generation Y and generation X.
Practical implications-Age plays a huge role in understanding the landing page leading to better purchase intention. Hence it is evident that a landing page that caters to all generations unambiguously has to be considered by marketers to boost sales.
Originality /value- The research results suggest that every generation seeks exclusivity on the landing page. So, generation-based perceived interactivity features on the landing page can attract all generations.
Keywords: Landing Page, Perceived Interactivity, Generation X, Generation Y, Generation Z and Alpha Generation.
Paper type-Research paper
The concomitant occurrence of hidradenitis suppurativa and Fournier’s gangrene
Authors: Khattar Georges , Bou Sanayeh Elie , Makram Marina , Rabah Hussein , Abu Baker Saif , Khan Salman , Hong Catherine 
Published: April 2024

Background: Fournier’s gangrene represents a life-threatening necrotising infection affecting the perineal region, while hidradenitis suppurativa is characterised by a chronic inflammatory skin condition. The simultaneous occurrence of both conditions is exceedingly rare. Case description: A 42-year-old female with a documented history of severe untreated hidradenitis suppurativa presented for shortness of breath, fever and lethargy, along with extensive wounds and skin breakdown involving the left axilla, perineum, lower back, lumbosacral region and bilateral gluteal areas, extending to the perineum. Upon presentation, the patient was in a state of septic shock, and a diagnosis of actively manifesting Fournier’s gangrene was established at the site of the pre-existing hidradenitis suppurativa lesions. Despite the implementation of an aggressive multidisciplinary approach incorporating surgical interventions, antibiotic therapy and intensive care measures, the patient’s condition deteriorated, culminating in septic shock, multi-organ failure and eventual demise. In this report, we discuss both clinical entities, their similarities and differences, and the possible mechanisms by which they may have co-occurred. Conclusion: The co-existence of hidradenitis suppurativa and Fournier’s gangrene poses unique challenges, given the rapid progression of Fournier’s gangrene within the context of hidradenitis suppurativa, potentially suggesting the latter as a predisposing factor. This case underscores the importance of vigilant screening and management of hidradenitis suppurativa.
UNVEILING FINANCIAL REALITIES: THE SUBSTANTIAL ECONOMIC IMPACT OF CENTRALIZED COMPLEX TIBIAL FRACTURE CARE IN THE UK
Authors: Jido Jamila Tukur , Al-Wizni Ahmed , Rodham Paul , Taylor David Martin , Kanakaris Nik , Harwood Paul 
Published: April 2024

IntroductionManagement of complex fractures poses a significant challenge. Evolving research and changes to national guidelines suggest better outcomes are achieved by transfer to specialist centres. The development of Major Trauma Networks was accompanied by relevant financial arrangements. These do not apply to patients with closed fractures referred for specialist treatment by similar pathways. Despite a surge in cases transferred for care, there is little information available regarding the financial impact on receiving institutions.Materials & MethodsThis retrospective study examines data from a Level 1 trauma centre. Patients were identified from our electronic referral system, used for all referrals. Transferred adult patients, undergoing definitive treatment of acute isolated closed tibial fractures, were included for a 2-year period. Data was collected using our clinical and Patient Level Information and Costing (PLICS) systems including coding, demographics, treatment, length of stay (LOS), total operative time, number of operations, direct healthcare costs, and NHS reimbursements.Results104 patients were identified, 23 patients were treated by internal fixation and 81 with circular frames. Patients required a median of 190 minutes of total operative time and 6 days of hospital stay at a median cost of £16,233 each, median reimbursement was £10,625. The total cost of treatment for all 104 patients was £2,205,611 and total reimbursement was £1,391463, the median deficit per patient being £5825. The overall deficit over the 2 years was £814,148.ConclusionsThis study reveals a considerable economic burden associated with treating complex tibial fractures. It should be emphasised that these do not include patients referred for fracture-related infection or non-union, who may also incur similar deficits in recovered costs. These findings emphasise the importance of understanding and addressing the financial implications of managing tertiary referral orthopaedic trauma patients to ensure efficient and sustainable resource allocation.
SELECTIVE REMOVAL OF ILIZAROV FRAMES WITHOUT A PERIOD OF DYNAMIZATION APPEARS SAFE
Authors: Brown Natasha , King Samuel , Taylor Martin , Foster Patrick , Harwood Paul 
Published: April 2024

IntroductionTraditionally, radiological union of fractures treated with an Ilizarov frame is confirmed by a period of dynamization - destabilisation of the frame for a period prior to removal. Reduced clinic availability during the COVID-19 pandemic caused a shift to selective dynamisation in our department, whereby lower risk patients had their frames removed on the same day as destabilisation. This study investigates the effects of this change in practice on outcomes and complication rates.Materials & MethodsAdult patients treated with circular frames between April 2020 and February 2022 were identified from our Ilizarov database. Patients were divided into 2 groups: - “dynamised” if their frame was destabilised for a period to confirm union prior to removal; or “not dynamised” if the decision was taken to remove the frame without a period of dynamisation, other than a short period in the clinic. A retrospective review of clinical notes was conducted to determine outcome.Results175 patients were included in the final analysis, 70 in the dynamised and 103 in the not dynamised groups, median follow-up was 33 months. 3 patients in the dynamised group failed dynamisation and had their period of fixation extended, subsequently having their frames removed without complication. Two patients suffered a refracture or non-union after frame removal in the dynamised group and none in the not dynamised group, this difference was not statistically significant.ConclusionsIn our practice, selective frame removal without a period of dynamisation appears safe. This has the potential to shorten frame time and reduce the number of clinic appointments and radiographic investigations for these patients. Some patients find the period of dynamisation uncomfortable and associated with pin site infection, which can be avoided. We plan to continue this practice and collect further data to confirm these findings in a larger dataset.
AN IN VITRO STUDY EXAMINING WIRE TENSION IN FINE WIRE CIRCULAR EXTERNAL FIXATION CONSTRUCTS
Authors: Howard Anthony , Harwood Paul , Benton Alice , Merrel Charlotte , Culmer Peter , Bolton William , Stewart Todd 
Published: April 2024

IntroductionIlizarov fixators are reliant on tensioned fine wires for stability. The tension in the wires is generated using specific tensioning devices. Loss of wire tension over time may lead of loss a stability and complications. A series of in vitro experiments were undertaken to explore wire tensioner accuracy, the impact of fixation bolt torque and initial tension on loss of tension in ilizarov constructs under static and dynamic loads.Materials & MethodsMedical grade materials were applied to a synthetic bone analogue using surgical instruments in all experiments. Bolt torque was fixed at 6, 10 or 14 Nm using a torque limiting wrench. Wire tension was assessed using a strain measurement bridge. Wires were tensioned to 90, 110 and 130kg as measured by a commercial dynamometric tensioner. Static and dynamic testing was undertaken using an instron testing machine. Cyclical loads from 50–750N were applied for 5000 cycles.ResultsActual wire tension was approximately 15% less than indicated by the tensioner device. Using fixation bolt torques of 10Nm and 14Nm achieved final wire tensions of around 60% and 80% of that applied at 90 and 130kg of applied tension. Static load testing demonstrated self stiffening to similar levels in all pre-tensions. Dynamic testing demonstrated significant loss of tension, most of which occured in the first 3 cycles, inversely proportional to the tension initially applied.ConclusionsThese experiments provides insight into the effect of initially applied wire tension on Illizarov mechanical performance. It is important surgeons understand how the different ways that these devices are applied affects mechanical performance. Further research examining what factors affect performance across different manufacturers equipment would therefore be relevant, alongside the development of novel fixation methods to reduce wire slippage and the further development of equipment for clinical use.
THE RESULTS OF TWO-RING HEXAPODS IN THE LOWER LIMB AT KING'S COLLEGE HOSPITAL
Authors: Macey Alistair , Omar Hussain Al , Leitch Patricia , Vaidean Traian , Swaine Suzanne , Santos Eliza , Bond Deborah , Abhishetty Naveen , Shetty Sandesh , Saini Aaron , Phillips Sarah , Groom Graeme , Lahoti Om 
Published: April 2024

IntroductionClassical fixation using a circular frame involves two rings per segment and in many units this remains the norm whether using ilizarov or hexapod type frames. We present the results of two ring circular frame at King's College Hospital.Materials & MethodsA prospective database has been maintained of all frames applied since 2007. Radiographs from frames applied prior to July 2022 were examined. Clinic letters were then used to identify complications.Included: two ring hexapod for fracture, malunion, nonunion, arthrodesis or deformity correction in the lower limb.Excluded: patients under 16 years old, diabetic feet, Charcot joints, soft tissue contractures, arthrodiastasis, correction of the mid/forefoot, plate fixation augmentation, fixation off a third ring.Results518 two ring hexapods were identified. Mean age was 46 (16–89). 55% were for fracture, 18% for malunion, 11% for nonunion. Mean frame time was 7 months (2–29 months).All clinic letters from 384 patients showed 203 patients (52%) had at least one pin site infection, 27 nonunions (7%), 16 frame revisions (4%), 25 bone grafting procedures (7%), 5 cases of septic arthritis (1%), 3 periprosthetic fractures (0.8%),), 12 malunions (3%), 4 Amputations (1%).ConclusionsThese results demonstrate the efficacy of a two ring hexapod for patients requiring reconstruction of the lower limb.
Historic Mosques in Sub-Saharan Africa: From Timbuktu to Zanzibar, Stéphane Pradines (2022)
Authors: Hillenbrand Robert 
Published: April 2024

Review of: Historic Mosques in Sub-Saharan Africa: From Timbuktu to Zanzibar, Stéphane Pradines (2022)
Leiden: Brill, 350 pp., 213 colour illus.,
ISBN: 9789004445543, $179 (hardback)
Exponential stability of non-conformable fractional-order systems
Authors: Echi Nadhem , Mabrouk Fehmi , Omri Faouzi 
Published: April 2024

Abstract
Recently, the authors Guzman et al. (2018) introduced a new simple well-behaved definition
of the fractional derivative called non-conformable fractional derivative. In this paper we study the exponential stability of non-conformable
fractional-order systems by using the Lyapunov function and Gronwall inequality.
These inequalities can be used as handy tools to research stability problems of nonlinear systems.
Sufficient conditions for exponential stability
are given using the Lyapunov theory. Further, deals with the state feedback stabilization problems for a family of nonlinear systems satisfying a Lipschitz continuity condition. The stability of the controller is proved by means of the new Lyapunov stability theorem given in this paper. A numerical example is given to illustrate the efficiency of the obtained result.
On a new variant of cyclic (noncyclic) condensing operators with existence of optimal solutions to an FDE
Authors: Khokhar Gurpreet Kaur , Gabeleh Moosa , Patel Deepesh Kumar 
Published: April 2024

Abstract
The paper is designed to highlight the presence of an optimal solution to a differential system comprising fractional order derivatives, precisely the ψ-Caputo derivative, under the prescribed initial constraints. This is attained through a new variant of cyclic (noncyclic) condensing operators incorporating a pair of maps. The altered notion facilitates in evolving best proximity point (pair) results as well as coupled best proximity point results with supportive corollaries.
Weighted integrability of Laguerre–Bessel transforms
Authors: Rakhimi Larbi , Daher Radouan , El Hamma Mohamed 
Published: April 2024

Abstract
The problem of weighted integrability of the Laguerre–Bessel transform in terms of the moduli of smoothness related to generalized translations is considered. Sufficient conditions are given to solve this problem. These results generalize a famous Titchmarsh’s theorem, due to [L. Rakhimi and R. Daher,
An analog of Titchmarsh’s theorem for the Laguerre–Bessel transform,
Arab. J. Math. Sci. 2023, 10.1108/AJMS-04-2022-0101] in the Laguerre–Bessel hypergroup. Also some results connected with the integrability of Laguerre–Bessel transforms are given.
A weakly inhomogeneous vibrating membrane and the solotone effect in two dimensions
Authors: Bason Gregory L. 
Published: April 2024

Abstract
The phrase solotone effect refers to a persistent irregular pattern within a set of eigenvalues. This effect arises when the underlying differential equations contain discontinuities. Models of the Earth contain such discontinuities and research into solotones was originally motivated by vibration problems in geophysics. More recently, solotone effects have been discovered within heat conduction, and within quantum wells. However, at present, only one-dimensional arrangements have been shown to give rise to a solotone effect. In this paper, we compute the eigenvalues of an inhomogeneous vibrating rectangular membrane. The resulting eigenspectra, when taken as a whole, fail to display a solotone effect. Nevertheless, we show that irregular patterns do exist within selected subsets of eigenvalues. Our analysis therefore provides the first example of a solotone effect in a spatial dimension greater than one. Furthermore, this discovery could be used to extend the solotone inverse method already implemented in one dimension.