bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes
Authors: Tang Xue , Ma Haitian , Huang Guoqing , Miao Jinfeng , Zou Sixiang 
Published: March 2009

AbstractThe effects of dehydroepiandrosterone (DHEA) on lipogenic gene mRNA expression were examined using cultured primary chicken hepatocytes. Cell samples were equilibrated to culture conditions for 24 h and then exposed to DHEA (1, 10 and 100 µM) dissolved in DMSO. The expression of sterol regulatory element‐binding protein‐1 (SREBP‐1) mRNA in cultured primary hepatocytes did not vary with time, except for a significant decrease in gene expression when cells were treated with 10 or 100 µM DHEA for either 1 or 2 h. A similar tendency toward decreased gene expression was evident for acetyl CoA carboxylase. The expression of peroxisome proliferator‐activated receptor α (PPARα) and carnitine palmitoyl transferase I mRNA was dramatically enhanced subsequent to treatment with 10 or 100 µM DHEA for periods of time from 6 to 48 h, while there was a decrease in the expression of PPARα in the presence of 10 µM DHEA after 1 and 2 h of exposure, and in the presence of 100 µM DHEA after 2 h of exposure. Hepatocytes treated with 10 or 100 µM DHEA contained more mitochondria and mitochondria with a higher electron density than did untreated hepatocytes. Furthermore, cell survival was significantly inhibited by treatment with 100 µM DHEA at 24, 48 and 72 h. In contrast, 1 µM DHEA administration significantly increased cell survival after 72 h; however, 10 µM DHEA treatment had no pronounced effect on cell survival. Overall, the results reported here indicate that DHEA accelerates lipid catabolism by direct regulation of hepatic gene expression and by induction of changes in hepatocyte mitochondria.
Cyclic Endiamino Peptides: A New Synthesis of Imidazopyrazines
Authors: Kogon Yael , Goren Lee , Pappo Doron , Rudi Amira , Kashman Yoel 
Published: March 2009

AbstractBy applying the cyclic endiamino cyclisation methodology to linear tripeptides embodying a β‐turn‐directing amino acid, the imidazopyrazine ring system was afforded. A mechanism involving the originally desired nine‐membered cyclic endiamine is suggested. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Electrochemical Cholesterol Sensor Based on Tin Oxide‐Chitosan Nanobiocomposite Film
Authors: Ansari Anees A. , Kaushik Ajeet , Solanki Pratima R. , Malhotra B. D. 
Published: March 2009

AbstractA chitosan (CS)‐tin oxide (SnO2) nanobiocomposite film has been deposited onto an indium‐tin‐oxide glass plate to immobilize cholesterol oxidase (ChOx) for cholesterol detection. The value of the Michaelis–Menten constant (Km) obtained as 3.8 mM for ChOx/CS‐SnO2/ITO is lower (8 mM) than that of a ChOx/CS/ITO bioelectrode revealing enhancement in affinity and/or activity of ChOx towards cholesterol and also revealing strong binding of ChOx onto CS‐SnO2/ITO electrode. This ChOx/CS‐SnO2/ITO cholesterol sensor retains 95% of enzyme activity after 4–6 weeks at 4 °C with response time of 5 s, sensitivity of 34.7 μA/mg dL−1 cm2 and detection limit of 5 mg/dL.
Disposable Electrodes for Capacitive Immunosensor
Authors: Teeparuksapun Kosin , Kanatharana Proespichaya , Limbut Warakorn , Thammakhet Chongdee , Asawatreratanakul Punnee , Mattiasson Bo , Wongkittisuksa Booncharoen , Limsakul Chusak , Thavarungkul Panote 
Published: March 2009

AbstractDisposable electrodes were fabricated by coating chromium (5 nm) and gold (200 nm) on glass strips (5.0 mm×25.4 mm) and used in a label‐free immunosensor. Human serum albumin (HSA) and its antigen (anti‐HSA) were used as a model system. Electropolymerization of o‐phenylenediamine was used for the immobilization of anti‐HSA by covalent binding. A linear relationship was obtained in the range from 1.0×10−14 to 1.0×10−9 M with a limit of detection of 8.0×10−15 M. Each modified electrode can be reused up to 30 times. The developed system was applied for human serum samples and compared to Albumin BCG method.
Comparison of DNA‐Reactive Metabolites from Nitrosamine and Styrene Using Voltammetric DNA/Microsomes Sensors
Authors: Krishnan Sadagopan , Bajrami Besnik , Mani Vigneshwaran , Pan Shenmin , Rusling James F. 
Published: March 2009

AbstractVoltammetric sensors made with films of polyions, double‐stranded DNA and liver microsomes adsorbed layer‐by‐layer onto pyrolytic graphite electrodes were evaluated for reactive metabolite screening. This approach features simple, inexpensive screening without enzyme purification for applications in drug or environmental chemical development. Cytochrome P450 enzymes (CYPs) in the liver microsomes were activated by an NADPH regenerating system or by electrolysis to metabolize model carcinogenic compounds nitrosamine and styrene. Reactive metabolites formed in the films were trapped as adducts with nucleobases on DNA. The DNA damage was detected by square‐wave voltammetry (SWV) using Ru(bpy)$\rm{ {_{3}^{2+}}}$ as a DNA‐oxidation catalyst. These sensors showed a larger rate of increase in signal vs. reaction time for a highly toxic nitrosamine than for the moderately toxic styrene due to more rapid reactive metabolite‐DNA adduct formation. Results were consistent with reported in vivo TD50 data for the formation of liver tumors in rats. Analogous polyion/ liver microsome films prepared on 500 nm silica nanoparticles (nanoreactors) and reacted with nitrosamine or styrene, provided LC‐MS or GC analyses of metabolite formation rates that correlated well with sensor response.
A Novel Thin‐Film Copper Array Based on an Organic/Inorganic Sensor Hybrid: Microfabrication, Potentiometric Characterization, and Flow‐Injection Analysis Application
Authors: Arida Hassan , Turek Monika , Rolka David , Schöning Michael J. 
Published: March 2009

AbstractA first step towards the microfabrication of a thin‐film array based on an organic/inorganic sensor hybrid has been realized. The inorganic microsensor part incorporates a sensor membrane based on a chalcogenide glass material (Cu‐Ag‐As‐Se) prepared by pulsed laser deposition technique (PLD) combined with an PVC organic membrane‐based organic microsensor part that includes an o‐xylyene bis(N,N‐diisobutyl‐dithiocarbamate) ionophore. Both types of materials have been electrochemically evaluated as sensing materials for copper(II) ions. The integrated hybrid sensor array based on these sensing materials provides a linear Nernstian response covering the range 1×10−6–1×10−1 mol L−1 of copper(II) ion concentration with a fast, reliable and reproducible response. The merit offered by the new type of thin‐film hybrid array includes the high selectivity feature of the organic membrane‐based thin‐film microsensor part in addition to the high stability of the inorganic thin‐film microsensor part. Moreover, the thin‐film sensor hybrid has been successfully applied in flow‐injection analysis (FIA) for the determination of copper(II) ions using a miniaturized home‐made flow‐through cell. Realization of the organic/inorganic thin‐film sensor hybrid array facilitates the development of a promising sophisticated electronic tongue for recognition and classification of various liquid media.
Fluorescent isotope‐coded affinity tag 2: Peptide labeling and affinity capture
Authors: Rivera‐Monroy Zuly , Bonn Guenther K. , Guttman András 
Published: March 2009

AbstractFluorescent isotope‐coded affinity tag (FCAT) is a novel reagent to label cysteine‐containing peptides. The fluorescein group on the tag enables absolute quantification by fluorescence detection, also supporting affinity capture of the labeled peptides. In this paper we report the synthesis of the heavy isotopic form of the FCAT reagent and its use in labeling tryptic peptides. The heavy form of the reagent exhibited the same reactivity and chromatographic behavior that of the light version. Effective labeling of tryptic peptides from α‐lactalbumin, fetuin, BSA and phosphorylase b was attained by using both the heavy and light FCAT tags. Selective capture of the FCAT‐labeled peptides was easily performed by pipette tips containing either anti‐FITC antibody or iminodiacetic‐acid‐coated beads. The differently labeled peptides were separated by RP HPLC and analyzed by MALDI‐TOF MS.
Intersymbol interference in DQPSK fibre‐optic systems
Authors: Zhao Hongxia , Agrell Erik , Karlsson Magnus 
Published: March 2009

AbstractThe limited bandwidth of high‐speed transmitter and receiver hardware gives rise to intersymbol interference (ISI) in digital communication systems. The influence of such ISI is investigated in a low‐complexity fibre‐optical setting, using differential quaternary phase‐shift keying (DQPSK) modulation. The ISI is approximated by a memoryless, stochastic model, for use in applications where neither equalisation nor sequence detection can be afforded. The channel capacity of this model is calculated and shown to depend strongly on the transmitter calibration. More than 3dB is gained, at a target bit error rate (BER) of 10−6, by allowing the transmitted phase levels to deviate from their nominal multiples of π/2 by up to 7%. Copyright © 2009 John Wiley & Sons, Ltd.
A hybrid FVM–LBM method for single and multi‐fluid compressible flow problems
Authors: Joshi Himanshu , Agarwal Arpit , Puranik Bhalchandra , Shu Chang , Agrawal Amit 
Published: March 2009

AbstractThe lattice Boltzmann method (LBM) has established itself as an alternative approach to solve the fluid flow equations. In this work we combine LBM with the conventional finite volume method (FVM), and propose a non‐iterative hybrid method for the simulation of compressible flows. LBM is used to calculate the inter‐cell face fluxes and FVM is used to calculate the node parameters. The hybrid method is benchmarked for several one‐dimensional and two‐dimensional test cases. The results obtained by the hybrid method show a steeper and more accurate shock profile as compared with the results obtained by the widely used Godunov scheme or by a representative flux vector splitting scheme. Additional features of the proposed scheme are that it can be implemented on a non‐uniform grid, study of multi‐fluid problems is possible, and it is easily extendable to multi‐dimensions. These features have been demonstrated in this work. The proposed method is therefore robust and can possibly be applied to a variety of compressible flow situations. Copyright © 2009 John Wiley & Sons, Ltd.
Predictors of neuropsychiatric symptoms in nursing home patients: influence of gender and dementia severity
Authors: Zuidema Sytse U. , de Jonghe Jos F. M. , Verhey Frans R. J. , Koopmans Raymond T. C. M. 
Published: March 2009

AbstractIntroductionThe aim of the study was to assess the influence of dementia severity and gender on neuropsychiatric symptoms in demented nursing home patients.MethodsNeuropsychiatric symptoms were assessed in a large sample of 1319 Dutch nursing home patients using the Cohen‐Mansfield Agitation Inventory (CMAI) and the Neuropsychiatric Inventory–Nursing Home version (NPI‐NH). The influence of gender and severity of cognitive decline, assessed with the Global Deterioration Scale (GDS), was investigated using logistic regression analysis and subsequently corrected for possible confounders, such as age, duration of institutionalization and psychoactive medication use.ResultsWhile physically aggressive behaviour was more common in patients with very severe cognitive deterioration (GDS stage 7), disinhibition, irritability, physically non‐aggressive and verbally agitated behaviour were more common in patients in GDS stage 5 or 6. Physically aggressive behaviour was more common in men, whereas female patients demonstrated more verbally agitated behaviour. With respect to other neuropsychiatric symptoms, delusions and depression were also more common in patients in GDS stage 5 and 6, while prevalences of anxiety and apathy further increased in severely demented patients (GDS stage 7). Apathy was more prevalent in male patients, while depression and anxiety were more common in females.ConclusionIn nursing home patients, neuropsychiatric symptoms were associated with the severity of dementia, with most symptoms occurring in patients showing (moderately) severe cognitive decline. Only physical aggression, anxiety and apathy were more common in patients with very severe cognitive decline. Dementia severity and gender were important predictors of neuropsychiatric symptoms in this patient cohort. Copyright © 2009 John Wiley & Sons, Ltd.
Use of physical restraints and antipsychotic medications in nursing homes: a cross‐national study
Authors: Feng Zhanlian , Hirdes John P. , Smith Trevor F. , Finne‐Soveri Harriet , Chi Iris , Du Pasquier Jean‐Noel , Gilgen Ruedi , Ikegami Naoki , Mor Vincent 
Published: March 2009

AbstractObjectivesThis study compares inter‐ and intra‐country differences in the prevalence of physical restraints and antipsychotic medications in nursing homes, and examines aggregated resident conditions and organizational characteristics correlated with these treatments.MethodsPopulation‐based, cross‐sectional data were collected using a standardized Resident Assessment Instrument (RAI) from 14,504 long‐term care facilities providing nursing home level services in five countries participating in the interRAI consortium, including Canada, Finland, Hong Kong (Special Administrative Region, China), Switzerland, and the United States. Facility‐level prevalence rates of physical restraints and antipsychotic use were examined both between and within the study countries.ResultsThe prevalence of physical restraint use varied more than five‐fold across the study countries, from an average 6% in Switzerland, 9% in the US, 20% in Hong Kong, 28% in Finland, and over 31% in Canada. The prevalence of antipsychotic use ranged from 11% in Hong Kong, between 26–27% in Canada and the US, 34% in Switzerland, and nearly 38% in Finland. Within each country, substantial variations existed across facilities in both physical restraint and antipsychotic use rates. In all countries, neither facility case mix nor organizational characteristics were particularly predictive of the prevalence of either treatment.ConclusionsThere exists large, unexplained variability in the prevalence of physical restraint and antipsychotic use in nursing home facilities both between and within countries. Since restraints and antipsychotics are associated with adverse outcomes, it is important to understand the idiosyncratic factors specific to each country that contribute to variation in use rates. Copyright © 2009 John Wiley & Sons, Ltd.
Buried anterolateral thigh flap for pharyngoesophageal reconstruction: Our method for monitoring
Authors: Spyropoulou Georgia‐Alexandra Ch. , Kuo Yur‐Ren , Chien Chih‐Yen , Yang Johnson Chia‐Shen , Jeng Seng‐Feng 
Published: March 2009

AbstractBackground.A noninvasive method for monitoring in cases of pharyngoesophageal defects reconstruction with the anterolateral thigh flap is presented.Methods.Seventeen patients underwent reconstruction from 2005 to 2007. In 16 patients, the distal stump of the descending branch of lateral circumflex femoral artery (LCFA) was left on the skin surface covered with a transparent film dressing and monitoring was performed by direct observation of the pulsation. In the 17th patient, the pedicle emerged from the transverse branch of the LCFA and dissection of the distal stump for monitoring was impossible; therefore, he was excluded from the study.Results.Cessation of the pulsation of the stump was noticed immediately postoperatively in 1 patient because of occlusion of the arterial anastomosis and in a second case 15 hours postoperatively due to a neck hematoma that caused collapse of the vein of the pedicle. Both flaps were salvaged.Conclusion.The proposed method of monitoring is simple, reliable, costless, and easily interpreted by the nursing staff. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Preclinical models of HPV+ and HPV− HNSCC in mice: An immune clearance of HPV+ HNSCC
Authors: Williams Robin , Lee Dong Wook , Elzey Bennett D. , Anderson Mary E. , Hostager Bruce S. , Lee John H. 
Published: March 2009

AbstractBackground.To investigate whether human papilloma virus (HPV)‐specific immune mechanisms can result in tumor clearance, we have created HPV+ and HPV− tonsil cells that form squamous cancers in immune‐competent mice. Here, we determine that an immune‐specific response can clear HPV+ tumor cells and the cellular requirements to mediate this tumor clearance.Methods.Through the benefit of this model, we use in vitro and in vivo methods to better understand how HPV+ cells are rejected.Results.The data show that an in vivo antigen‐specific antitumor response is generated to HPV+ transformed cells and that this response requires CD4+ and CD8+ cells to mount this antitumor response.Conclusion.The findings from this preclinical model will have implications not only in understanding human disease but also as a valuable model for testing immunotherapeutic strategies for HPV+ head and neck cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Impact of an integrated care program for patients with head and neck cancer on the quality of care
Authors: Ouwens Mariëlle M. M. T. J. , Hermens Rosella R. P. M. G. , Hulscher Marlies M. E. J. L. , Merkx Matthias A. W. , van den Hoogen Frank J. A. , Grol Richard P. , Wollersheim Hub C. H. , Marres Henri A. M. 
Published: March 2009

AbstractBackground.The management of patients with head and neck cancer is complex, and implementation of an integrated care program might improve the quality of care.Methods.A prospective before–after study was performed in 1 clinic for head and neck oncology on 311 adults with head and neck cancer to evaluate an integrated care program.Results.Scores on the integrated care indicators showed that the implementation of the integrated care program led to relevant improvements, eg, waiting time for diagnostic procedures less than 10 days (improvement of 37%), support for stopping smoking (+37%), nutrition support (+44%), assessment of CT and MRI scans by a an expert radiologist (+23%), and number of patients in contact with the specialist nurses (+37%). The program had no relevant effects on the outcome indicators.Conclusion.An integrated care program can improve several aspects of the management of patients with head and neck cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Quality of life in patients with maxillectomy prostheses
Authors: Irish Jonathan , Sandhu Nimani , Simpson Colleen , Wood Robert , Gilbert Ralph , Gullane Patrick , Brown Dale , Goldstein David , Devins Gerald , Barker Emma 
Published: March 2009

AbstractBackground.This cross‐sectional study sought to determine patient quality of life and function after prosthetic rehabilitation for maxillary and palate defects following cancer resection.Methods.Sixty‐nine patients were identified, 42 participated in the study (61%). The Obturator Functioning Scale (OFS) and 4 general quality of life measures (Mental Health Inventory [MHI], Impact of Events Scale [IES], Illness Intrusiveness Ratings Scale [IIRS], and Centre for Epidemiologic Studies Depression Scale [CES‐D]) were correlated with clinical parameters.Results.Leakage when swallowing foods was the most frequently reported problem with the obturator (29%). Positive correlation was noted between the OFS and both the IES subscales (p < .01) and CES‐D (p < .001). Difficulty with speech and eating was associated with increased avoidance of social situations. The surgical approach had a significant effect on the OFS, IES, and MHI subscales (p < .01).Conclusion.These results support the findings that good obturator function is associated with a better quality of life. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Cetuximab in hemodialysis: A case report
Authors: Aldoss Ibrahim T. , Plumb Troy , Zhen Weining (Ken) , Lydiatt Daniel D. , Ganti Apar Kishor 
Published: March 2009

AbstractBackground.Concurrent chemoradiotherapy with cisplatin is the standard therapy for patients with unresectable locally advanced head and neck squamous cell carcinoma. However, cisplatin administration in patients on hemodialysis is complicated by the need to perform hemodialysis immediately after the infusion. Concurrent chemoradiation with cetuximab has been approved in definitive treatment of locally advanced head and neck cancer. Although cetuximab is not excreted via the kidneys, its use in patients on hemodialysis has not been reported.Methods and Results.We present the case of a 65‐year‐old man undergoing hemodialysis, with stage IVA squamous cell carcinoma of the hypopharynx. Given the logistics of performing hemodialysis immediately postcisplatin, he received concurrent chemoradiotherapy with cetuximab. He tolerated treatment well with minor side effects.Conclusion.Cetuximab can be safely used in patients with renal impairment. This is the first reported case of the use of cetuximab in a patient undergoing hemodialysis. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
COX‐2polymorphisms and the risk for head and neck cancer in white patients
Authors: Peters Wilbert H. M. , Lacko Martin , te Morsche Rene H. M. , Voogd Adri C. , Oude Ophuis Michael B. , Manni Johannes J. 
Published: March 2009

AbstractBackground.Cyclooxygenase‐2 (COX‐2) is an enzyme involved in the synthesis of prostaglandins and thromboxanes, which are regulators of processes such as inflammation, cell proliferation, and angiogenesis, all relevant for cancer development. We investigated whether functional genetic polymorphisms inCOX‐2may have a risk‐modifying effect on head and neck carcinogenesis.Methods.Blood from 431 white patients with oral, pharyngeal, or laryngeal carcinoma and 438 white healthy controls was investigated for the presence of 2 functional promoter region polymorphisms (−1195A→Gand −765G→C) inCOX‐2.Results.Logistic regression analysis did not show differences inCOX‐2genotype distributions between patients and controls. Also no differences were found when stratified according to tumor localization, sex, or tobacco consumption.Conclusion.In contrast to earlier reports on the role of theseCOX‐2polymorphisms in mediating susceptibility to squamous esophageal carcinoma in a Chinese population, we could not demonstrate a risk‐modifying effect in head and neck carcinogenesis in whites. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Pseudogout mimicking an infratemporal fossa tumor
Authors: Kalish Larry H. , Ng Thomas , Kalnins Ian , Da Cruz Melville J. 
Published: March 2009

AbstractBackground.The infratemporal fossa is a well‐concealed, complex anatomical space. Neoplasms arising in this area are heterogenous in nature and have an insidious onset and usually present late.Methods and Results.We present a case of a 71‐year‐old woman who presented with worsening facial pain, trismus, and a large mass in her infratemporal fossa with minimal associated temporomandibular joint destruction. She underwent a surgical excision of the mass, which revealed a pseudogout deposit.Conclusion.The clinical and radiological features of patients with tophaceous pseudogout frequently mimic those of a benign or malignant neoplasm of the infratemporal fossa, often resulting in more radical surgery. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Arteriovenous malformation in the parotid region presenting as pulsatile tinnitus: A case report
Authors: Chen Meng‐Chao , Chung Wen‐Yuh , Luo Chao‐Bao , Wu Hsiu‐Mei 
Published: March 2009

AbstractBackground.Pulsatile tinnitus is a unique symptom in the general population and often leads patients to medical attention.Methods and Results.We report a patient who had an arteriovenous malformation of superficial temporal artery in the parotid region causing pulsatile tinnitus and insomnia. Magnetic resonance angiography and carotid angiography were useful tools for the detection of this vascular malformation. Successful treatment of this lesion was achieved by endovascular embolization.Conclusion.This case illustrates a thorough diagnostic work‐up with a high index of suspicion and a proper treatment option is rewarding when dealing with such a rare disease. © 2009 Wiley Periodicals, Inc. Head Neck, 2010