bluuebunny/crossref_metadata_2025_split huggingface dataset:
The MYB-related transcription factor MYPOP acts as a selective regulator of cancer cell growth
Authors: Florin Luise , Strunk Johannes , Hüppner Alena , Sial Mahwish , Plath Matthias , Wagner Sascha , Freitag Kirsten , Mikuličić Snježana , Bopp Tobias , Klein Matthias , Rajalingam Krishnaraj , Harms Gregory , Marini Federico , Ludt Annekathrin , Hankeln Thomas , Osterhof Carina , Henrich Alina , Nubbemeyer Andrea , Suchan Martin , Schrörs Barbara , Kreiter Sebastian , Kölsch Anne , Diken Mustafa , Döring Tatjana 
Published: March 2024

Abstract

The MYB-related transcription factor and partner of profilin (MYPOP or p42POP) is a ubiquitously expressed and understudied protein, recently discovered in restricting oncogenic human papillomaviruses (HPV) and suggested as a tumor suppressor. In this study, we investigate the role of MYPOP on cancer cells. At supra-physiological levels, induced by both plasmid DNA- and messenger RNA-mediated gene transfer, MYPOP emerges as a potent tumor growth inhibitor, capable of inducing cancer cell death while sparing normal cells. Using HPV-transformed cervical cancer cells and normal human epidermal keratinocytes, cell behavior assessments as well as transcriptome analysis revealed MYPOP's specific anti-proliferative and death-inducing impact on cancer cells. We found MYPOP capable of silencing viral and human oncogenes includingE6,E7, andMYC, and of triggering the release of the cancer-killing cytokine interleukin-24. Extending our research to murine Mypop, we observed anti-proliferative effects in mouse melanoma and colorectal cancer cells. Collectively, our findings underscore MYPOP's potential as a selective tumor suppressor in both human and mouse cancer cells, opening a promising avenue for futurein vivostudies.
Membrane potential states gate synaptic consolidation in human neocortex
Authors: Mittermaier Franz , Kalbhenn Thilo , Xu Ran , Onken Julia , Faust Katharina , Sauvigny Thomas , Thomale Ulrich , Kaindl Angela , Holtkamp Martin , Grosser Sabine , Fidzinski Pawel , Simon Matthias , Alle Henrik , Geiger Jörg 
Published: March 2024

Abstract

Synaptic mechanisms that contribute to memory consolidation in the human brain remain largely unexplored. The formation of lasting memories critically relies on sleep1-3. During slow wave sleep, neocortical neurons exhibit characteristic membrane potential oscillations known as UP and DOWN states4-7. In this study, we show that sleep-like UP and DOWN states modulate axonal action potentials and temporarily enhance synaptic transmission between human layer 2 & 3 pyramidal neurons. In convergent microcircuit motifs, synaptic enhancement by synchronous UP and DOWN states leads to efficient recruitment of postsynaptic action potentials, which in turn results in long-term stabilization of synaptic strength. In contrast, synapses undergo lasting depression if presynaptic neurons fail to recruit postsynaptic action potentials. Taken together, our study offers a mechanistic framework in humans that can explain how precise coupling of neural activity to slow waves gates synaptic plasticity, which is the substrate of memory consolidation8-12. This framework could inspire improved brain stimulation strategies targeting memory performance13-18.
Chromosome-level genome assembly of a cliff plant Taihangia rupestris var. ciliata provides insights into its adaptation and demographic history
Authors: Li Wei-Guo , Li Yuan-Yuan , Zheng Chuan-Kun , Li Zhi-Zhong 
Published: March 2024

Abstract
Background Cliffs are recognized as one of the most challenging environments for plants, characterized by harsh conditions such as drought, infertile soil, and steep terrain. However, they surprisingly host ancient and diverse plant communities and play a crucial role in protecting biodiversity. The Taihang Mountains, which act as a natural boundary in eastern China, support a rich variety of plant species, including many unique to cliff habitats. However, it is little known how cliff plants adapt to harsh habitats and the demographic history in this region.
Results To better understand the demographic history and adaptation of cliff plants in this area, we analyzed the chromosome-level genome of a representative cliff plant, T. rupestris var. ciliata, which has a genome size of 769.5 Mb, with a scaffold N50 of 104.92 Mb. The rapid expansion of transposable elements may have contributed to the increasing genome and its ability to adapt to unique and challenging cliff habitats. Comparative analysis of the genome evolution between Taihangia and non-cliff plants in Rosaceae revealed a significant expansion of gene families associated with oxidative phosphorylation, which is likely a response to the abiotic stresses faced by cliff plants. This expansion may explain the long-term adaptation of Taihangia to harsh cliff environments. The effective population size of the two varieties has continuously decreased due to climatic fluctuations during the Quaternary period. Furthermore, significant differences in gene expression between the two varieties may explain the varied leaf phenotypes and adaptations to harsh conditions in different natural distributions.
Conclusion our study highlights the extraordinary adaptation of T. rupestrisvar. ciliata, shedding light on the evolution of cliff plants worldwide.
Enhancing breast cancer detection in PTEN Hamartoma Tumor Syndrome through insights into MRI and mammography lesion features
Authors: Mann Ritse , Hoxhaj Alma , Milants Annemieke , Techanithisawat Porjai , Bult Peter , Hoogerbrugge Nicoline 
Published: March 2024

Abstract

Women with PTEN Hamartoma Tumor Syndrome (PHTS) have an increased breast cancer (BC) risk (up to 66%) and a relatively high prevalence of benign breast lesions (BBLs) (30–75%), necessitating annual MRI and mammography surveillance. This study aimed to describe BCs and BBLs imaging features in PHTS women to improve early BC detection. This retrospective study at Radboudumc (2001–2021) involved 65 PHTS women aged ≥ 18. Of these, 39 participated in a high-risk BC surveillance program. Breast lesion features at MRI and mammography (when available) examinations were described by two breast radiologists. Pathology reports were retrieved to assess the nature of the biopsied findings. Statistical analysis included descriptive statistics and correlation analyses, with significance set at P < 0.05. Twenty-one women were diagnosed with 35 BCs. For 11 women (52%), who were diagnosed with 17 BCs (49%), imaging examinations were available for re-reading. Of these 17 BCs, 12 (70%) were invasive. MRI examinations were available for re-reading for 10 BCs, with MRI detecting 9 of these (sensitivity 90%). The BCs identified by MRI primarily exhibited malignant features. Notably, up to 50% of BCs were visible in baseline MRI examinations. Mammography examinations were available for re-reading for 15 BCs, with mammography detecting 6 of these (sensitivity 40%). Eighty-nine BBLs were diagnosed in 23 women. Imaging examinations were available for re-reading for 31 BBLs in 16 women. MRI detected 29 (100%) out of 29 available BBLs cases, while mammography detected 15 (58%) out of 26 BBLs available cases. On MRI, BBLs often showed ambiguous enhancement features. In our study, MRI identified BCs mostly exhibited malignant features, while mammography often missed cancers. On MRI, most BCs were already visible at baseline examinations, and BBLs showed ambiguous enhancement features. These findings highlight the critical role of thorough MRI evaluation in the detection of BCs in PHTS women.
A bivalent histone mark reader, AtDEK2 governs plant immunity
Authors: Rayapuram Naganand , Alhoraibi Hanna , Alejandro-Martinez Santiago , Latrasse David , Mandal Papita , Faivre Lea , He Xiaoning , Mianza Déborah Manza , Abulfaraj Aala , Alhrabi Siba , Mariappan Kiruthiga , Artyukh Olga , Abdulhakim Fatimah , Aljedaani Fatimah , David Stephan , Almeida-Trapp Marilia , Bigeard Jean , Pflieger Delphine , Fischle Wolfgang , Arold Stefan , Colcombet Jean , Schubert Daniel , Benhamed Moussa , Blilou Ikram , Hirt Heribert 
Published: March 2024

Abstract
In Arabidopsis thaliana, the nuclear protein DEK2 orchestrates diverse chromatin-related processes and exhibits phosphorylation in response to flagellin22 treatment, implicating its involvement in plant immunity against bacterial pathogens. Loss-of-function mutants of dek2 have their immunity compromised to both bacterial and fungal pathogens. Transcriptomic analysis of the dek2-1 mutant unveils AtDEK2 as a transcriptional repressor of defense-related genes, as well as genes associated with hormone synthesis and signaling. Chromatin immunoprecipitation sequencing (ChIP-Seq) analysis reveals that DEK2 binds to motifs of various transcription factor families, with a notable enrichment in class I TCP binding motif regions. Our findings indicate that DEK2 is recruited to specific chromatin regions by transcription factors and functions as a reader of the bivalent histone mark H3K4me3K27me3. Consequently, we propose a hypothetical working model wherein DEK2 acts as a transcriptional repressor targeting regions marked by H3K4me3K27me3, shedding light on its role in plant immunity.
Online Reviews based Ranking of Hotels integrating Topic Modelling and Picture Fuzzy approach
Authors: Aggarwal Anu Gupta , Aggarwal Sanchita , Jindal Vinita 
Published: March 2024

Abstract
The hospitality sector generates a lot of data, which is added as written feedbacks and/or numerical ratings. Online travel agencies (OTAs) thrive on giving consumers a variety of options tailored according to their tastes and needs. In this research, we propose ranking of hotels according to customer provided reviews. The whole process is carried out using several techniques. The reviews are initially pre-processed and a topic modelling technique Latent Dirichlet Allocation (LDA) has been utilized to extract important features. Maximum Entropy Minimum Variance Ordered Weighted Averaging (MEMV-OWA) method is then utilized to assign weights to individual topics. For ranking, multi-criteria decision-modelling approach Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) is utilized. To address the imprecision inherent in human judgement, picture fuzzy numbers are used. The findings of the research provide theoretical and practical implementations and are discussed in the paper.
Water levels forecasting for the river basins of Kien Giang in Quang Binh province using Transformer-based approaches
Authors: Quoc Bao Bui , Khanh Hung Nguyen , Dac Hieu Nguyen , Anh Dat Tran , TA Quang Chieu 
Published: March 2024

Abstract
Precision and timely forecasting of water levels holds critical significance across diverse applications such as flood prediction, hydroelectric power management, and environmental surveillance. While conventional recurrent neural network (RNN)-based techniques have been extensively employed for this purpose, recent strides in long-term time-series forecasting have ushered in transformer-based models, showcasing remarkable advancements in predicting time-series data. This study delves into the utilization of transformer-based models for water level prediction, specifically concentrating on the Nhat Le River Basin. Through numerous experiments encompassing different scenarios and employing various model architectures, we offer detailed analyses of the model's predictive capabilities. Demonstrating consistent superiority over traditional RNN-based methods, the transformer-based models excel across various evaluation metrics, including root mean square error (RMSE), mean absolute error (MAE), and coefficient of determination (R2). Notably, these models exhibit outstanding precision in forecasting flood peaks, consistently maintaining errors below 0.02 meters. The resilience and scalability of transformer-based models position them as highly promising for achieving accurate water-level predictions in practical, real-world applications.
Qualitative Assessment of a virtual cardiovascular medical education Program in Haiti: local physicians in-training experiences
Authors: Ilali Marwa , Clavel Virginie , Panagiotou Veronika , Mengesha Mlka , Policard Giovanni Léon , Moïse Carmene Altagracia , Petit-Homme Tamara , Etienne David , Cadri Abdul , Dade Eliezer , Dawson Calixte , Saint-Joy Veauthyleau , Haynes Norrisa 
Published: March 2024

Abstract
Background: Haiti faces challenges in accessing equitable healthcare and medical education due to limited resources. Cardiovascular disease burden is high, necessitating a well-trained cardiovascular workforce. The International Cardiology Curriculum Accessible by Remote Distance Learning (ICARDs) program, launched in 2019, addresses this need by providing virtual cardiovascular education. This study aimed to explore the perceptions and experiences of internal medicine residents and physicians participating in the ICARDs program, focusing on their expectations, facilitators, barriers, and recommendations for improvement.
Study Design: A qualitative research approach was adopted, conducting three focus groups with participants in three different hospitals across Haiti.
Methods: The study utilized a Unified Theoretical Framework of Learning Theories as a structured framework to identify themes. Thematic analysis was employed to extract recurring patterns and valuable information from participants' responses.
Results: Participants expressed high expectations and reported positive experiences with the ICARDs program. They acknowledged its positive impact on patient care and the development of their medical skills. However, some concerns were raised regarding course content and irregularities in the program.
Conclusions: The ICARDs program fulfills participants' expectations for cardiovascular education, improves patient care, and fosters a supportive community. To enhance its effectiveness, addressing content concerns and infrastructure limitations is essential. The study's findings provide valuable insights for program organizers to tailor the ICARDs program and better meet the participants' needs.
Intrinsic Response of surface modified Calotropis Gigantea Fiber and Nelumbo nucifera filler hybrid composite
Authors: RAM GANESH , Arjunan T. V. , Kumar K. Vinoth , Loganathan T.G. 
Published: March 2024

Abstract

The present study used Calotropis Gigantea fiber, which has minuscule significance in vegetation value, and waste Nelumbo Nucifera filler for the production of polymer composites. The Calotropis gigantea yarn fiber is twisted into Two-Ply Yarn and subsequently woven into mats, the mat underwent both NaOH treatment and microwave irradiation in order to enhance their compatibility with epoxy. This work aims to examine the mechanical and dynamic properties of a hybrid composite material consisting of a Chemically and Microwave Treated Calotropis Gigantea (CMTCG) fiber combined with Nelumbo nucifera (NN) infills. The objective of this study is to investigate the possible improvement of the mechanical and dynamic characteristics of polymer composites by developing composite samples that include different weight proportions of NN fillers in CMTCG-reinforced Epoxy composites. The assessment of the mechanical properties of several combinations of CMTCG with NN infills was conducted utilizing established testing methodologies, including tensile, flexural, impact, and Dynamic Mechanical Analysis (DMA) to assess the viscoelastic nature of the composites. In addition to this, a Cole-Cole curve is plotted by utilizing the findings of the DMA to analyze the effect of particle impregnation and the anticipation of the heterogeneity of the composite material at different loadings of fillers. The current investigation revealed that a fiber loading of 10 weight percentages represented the optimal threshold, and any loading value resulted in a noticeable decline in all characteristics.
Medication Non-adherence and Associated Factors Among Patients with Mental Illness at St. Amanuel Specialized Psychiatric Hospital, Addis Ababa, Ethiopia; A Cross Sectional Study
Authors: Adane Adugnaw , Zerihun Diborah , Warkaw Adisu , Misganaw Samuel , Mulualem Solomon 
Published: March 2024

Abstract
Introduction:
Adherence is the degree to which patients follow health professionals’ recommendations regarding prescribed medication and maintain the indicated treatment. Medication non-adherence is a global problem worldwide. Medication non-adherence is a significant clinical concern in patients with mental illnesses than other chronic illness patients.
Objectives
To assess medication non-adherence and associated factor among patients with mental illness who visited St. Amanuel Specialized Psychiatric Hospital, Addis Ababa.
Methods
Institution-based cross-sectional study design was employed at St. Amanuel Specialized Psychiatric Hospital, Ethiopia from August 20, 2022 to September 30, 2022. The data was collected by interviewer administered structured questionnaire and document review. Medication non-adherence was assessed by using standardized Morisky medication adherence scale. The collected data was entered into EPI data version 4.5 and exported to SPSS version 26 for statistical analysis. After descriptive data was presented; Binary logistic regression was used to assess association. The significance level was set at P < 0.05 and Odds ratio was used to assess the strength of association.
Result
A total of 374 patients were included, of this 32.6% were found to have poor adherence. The predictors of poor adherence were male sex [AOR = 1.841, 95% CI; 1.136–2.984, p = 0.013], substance use [AOR = 2.088, 95% CI; 1.222–3.569, p = 0.008], unemployment [AOR = 2.593, 95% CI; 1.446–4.649, p = 0.021], perceived spiritual causation [AOR = 1.743, 95% CI; 1.026–2.959 p = 040], poor level of support [AOR = 3.277, 95% CI; 1.432–7.496, p = 0.005].
Conclusion
The magnitude of medication non-adherence was found to be high. Male sex, unemployment, substance use, perceived, and poor level of support were significant predictors of medication non-adherence among patients with mental illness.
Efficacy of Ultrasound Guided Erector Spinae Plane Block Compared to Wound Infiltration for Postoperative Analgesia in Laparoscopic Live Donor Nephrectomy: A Double-Blinded Randomized Controlled Trial
Authors: Loganathan Sekar , Kajal Kamal , Garg Kashish , Sethi Sameer , Kenwar Deepesh B , Aditya Ashish , Sharma Amit , Mahajan Varun , Naik Naveen B , Kumar Rajnikant 
Published: March 2024

Abstract

Background
Postoperative pain remains a significant problem in patients undergoing donor nephrectomy despite reduced tissue trauma following laparoscopic live donor nephrectomy (LLDN). Inadequately treated pain leads to physiological and psychological consequences, including chronic neuropathic pain.
Materials and Methods
This randomized controlled double-blinded trial was conducted in sixty-nine (n=69) participants who underwent LLDN under general anesthesia. Participants were randomized into Group B (n=34) and Group C (n=35). Group B received ultrasound-guided bilateral erector spinae plane block (ESPB) with bupivacaine 0.125% 20ml on the surgical side and 10ml on the contralateral side at T9-10 level before extubation, while Group C received wound infiltration with bupivacaine 0.125% 15ml. The primary objective of the study was to compare cumulative 24-hour morphine consumption postoperatively. The secondary objectives were time to first rescue analgesia, visual numeric rating scale (VNRS) pain scores at rest and during movement, incidence of postoperative nausea and vomiting (PONV), and complications associated with ESPB.
Results
Participants in Group B required significantly less median (IQR) 24-hour morphine compared to Group C [6 (6-9) mg vs 15 (12-15) mg; median difference 9; 95% CI in median difference 6-12; p<0.001), longer median (IQR) time to first rescue analgesia [6 (6-8) hours vs 1 (1-2) hours; p < 0.001], and lower VNRS at rest and during movement at baseline, 0.5, 1, 2, 4, 6, 8, 12, 24 hours.
Conclusion
Ultrasound-guided ESPB provided effective pain relief compared to wound infiltration with local anaesthetic in patients who underwent LLDN.
Trial Registration number: INT/IEC/2021/SPL-514; CTRI/2021/07/045909
Study of the effect of suspension training on the balance ability of surfers without relying on vision
Authors: Wang Zhaoyi , Ma Yong , Guo Zhi-Hao , Jia Meng-Yao , Zheng Wei-Tao 
Published: March 2024

Abstract

Surfing is an emerging Olympic sport that requires athletes to have excellent balance without relying on vision. This study introduces TRX suspension training (TRX) into the balance training of surfing programs to investigate its effectiveness on the surfers' balance ability without relying on vision. Thirty-two surfers from the National Surfing Team were randomly divided into a TRX group and a traditional balance training (TB) group, and the two groups were given intervention training for about 30 minutes three times a week for eight weeks. Eye-closed one-leg stand and linear travel deviation tests were performed at different experiment stages to examine static and dynamic balance changes without visualization. After eight weeks of intervention training, both TRX and TB were very effective in improving surfers' static balance without relying on vision (p < 0.01), and there was no significant difference in the effectiveness of the two training methods in improving surfers' static balance without relying on vision (pLeft=0.142 > 0.05, pRight=0.084 > 0.05). Additionally, the eight weeks of suspension and TB effectively improved the surfers' dynamic balance without relying on vision, and highly significant improvements were seen in each monitoring phase (p < 0.01). The effect of the two training methods on the improvement of surfers' dynamic balance without relying on vision began to show a significant difference after week five of training (p = 0.021 < 0.05) and a very significant difference after week eight (p = 0.000 < 0.01). The results demonstrated that TRX was more effective than TB in improving the athletes' balance ability. Therefore, both TRX and TB improved the surfers' non-vision-dependent balance ability very well. However, TRX was more effective in improving dynamic balance in that situation. TB and TRX can be used to improve the static balance ability and dynamic balance ability for the first five weeks, and TRX can be applied to the balance training of surfers after five weeks.
Merging DNA repair with bioorthogonal conjugation enables accessible and superior asymmetric DNA catalysis
Authors: Zhu Ru-Yi , Sheng Jie , Li Zhaoyang , Koh Kelly Kar Yun , Shi Qi , Foo Angel , Tan Philip Mark Leetiong , Kha Tuan-Khoa , Wang Xujie , Fang Leonard 
Published: March 2024

Abstract
Optimizing catalysts through high-throughput screen for asymmetric catalysis is challenging due to the difficulty associated with assembling a library of catalyst analogues in a timely fashion. Here, we repurpose DNA excision repair and integrate it with bioorthogonal conjugation to construct a diverse array of DNA hybrid catalysts for highly accessible and high-throughput asymmetric DNA catalysis, enabling dramatically expedited catalyst optimization process, superior reactivity and selectivity, as well as the first atroposelective DNA catalysis. The bioorthogonality of this conjugation strategy ensures exceptional tolerance towards diverse functional groups, thereby facilitating the facile construction of 42 DNA hybrid catalysts bearing various unprotected functional groups. This unique feature holds the potential to enable catalytic modalities in asymmetric DNA catalysis that were previously deemed unattainable.