bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hubungan Status Gizi dengan Konsentrasi Belajar Pada Anak di SDN 427 Malewong Kecamatan Larompong Selatan Kabupaten Luwu Provinsi Sulawesi Selatan
Authors: Sumarni  , Muh Alfian Jafar  , Irna Diyana Kartika K  , Nurul Fadilah Ali Polanunu  , Ida Royani  , Windy Nurul Aisyah  
Published: March 2024

Usia sekolah merupakan salah satu masa yang mengalami tumbuh kembang yang cepat. Status gizi dan kesehatan yang optimal pada usia sekolah sangat penting dijaga untuk membantu proses pertumbuhan dan perkembangan, berpikir, aktivitas fisik yang optimal serta menjaga daya tahan tubuh sehingga dapat menunjang kegiatan belajar di sekolah. Konsentrasi merupakan pemusatan pikiran dan perhatian terhadap suatu hal dengan menyampingkan semua hal lainnya yang tidak berhubungan. Kemampuan anak dalam berkonsentrasi akan mempengaruhi kecepatan dalam menangkap materi yang diberikan. Penelitian ini bertujuan untuk mengetahui hubungan status gizi dengan konsentrasi belajar pada anak di SDN 427 Malewong. Menggunakan metode penelitian kuantitatif menggunakan desain studi cross sectional. Teknik pengambilan sampel menggunakan total sampling menggunakan uji chi square. Hasil dari 32 responden dengan menggunakan uji chi square didapatkan nilai p-value =0,024 (p < 0,05).Kesimpulan terdapat hubungan antara status gizi dengan konsentrasi belajar pada anak di SDN 427 Malewong.
Hubungan Tingkat Pengetahuan dan Sikap terhadap Deteksi Dini Kanker Payudara pada Pegawai RS Ibnu Sina
Authors: Khaerunnisa Andi bulqis , Latief Shofiyah , Syahruddin Febie Irsandi , Royani Ida , Juhamran Reeny Purnamasari 
Published: March 2024

Kanker payudara merupakan keganasan pada jaringan payudara yang dapat berasal dari epitel duktus maupun lobulus. Ditinjau dari biomolekuler, kanker payudara merupakan penyakit akibat mutasi gen yang dipicu oleh multifaktor seperti faktor diet, faktor lingkungan, dan faktor keturunan yang dikenal sebagai faktor risiko. Dari data Global Burner of Cancer (GLOBOCAN) 2020, Penderita kanker payudara menempati urutan pertama kanker paling banyak pada wanita dengan presentasi kasus tertinggi dibanding dengan kanker lain yaitu 2,3 juta kasus dengan presentasi mortalitas sebesar 684,996 kasus pada wanita didunia, Berdasarkan data International Agency for Research on Cancer dari Word Health Organization (WHO) tahun 2020, penderita kanker tertinggi di Indonesia untuk wanita adalah kanker payudara yaitu sebesar 65.858 kasus dengan mortalitas sebesar 22.430 kasus. Tujuan untuk mengetahui hubungan tingkat pengetahuan dan sikap terhadap deteksi dini kanker payudara pada pegawai rumah sakit Ibnu Sina Makassar. Merupakan penelitian deskriptif dengan rancangan cross-sectional. Dari 40 Responden didapatkan hasil berdasarkan kategori tingkat pengetahuan, dengan pengetahuan cukup sebanyak 16 responden (40%), dan tingkat pengetahuan baik sebanyak 11 responden (27,5%), sikap kategori baik sebanyak 17 responden (42,5%) dan sikap kategori cukup sebanyak 11 responden (27,5%), dan deteksi dini kanker payudara yang tidak melakukan sebanayak 21 responden (52,5%), dan yang melakukan deteksi dini kanker payudara sebanyak 19 responden (47,5%). Terdapat hubungan antara tingkat pengetahuan terhadap deteksi dini kanker payudara p=0.002 dan terdapat Hubungan antara sikap terhadap deteksi dini kanker payudara p= 0.037 pada pegawai Wanita di Rumah Sakit Ibnu Sina Makassar
Uji Efektivitas Antijamur Ekstrak Bunga Bugenvil (Bougainvillea spectabilis Willd.) Terhadap Jamur Candida albicans (Robin Berkhout)
Authors: TRIYULIANI CIKMAH , Sariyanti Mardhatillah 
Published: March 2024

Latar Belakang: Salah satu penyakit infeksi akibat jamur adalah infeksi jamur yang disebabkan oleh spesies Candida albicans. Penggunaan ketokonazol sebagai antijamur untuk mengobati infeksi jamur Candida albicans memiliki keterbatasan seperti efek samping yang ditimbulkan hingga resistensi. Pemanfaatan tanaman obat merupakan salah satu alternatif untuk mengatasi masalah tersebut. Ekstrak bunga bugenvil merupakan salah satu tumbuhan yang memiliki banyak manfaat, salah satunya sebagai antijamur.
Metode: Penelitian ini menggunakan ekstraksi bunga bugenvil dilakukan menggunakan metode maserasi dengan pelarut etanol 96% dan dilarutkan dengan DMSO 5%. Hasil ekstraksi kemudian dilakukan uji Minimal Inhibitory Concentration (MIC) dengan variasi konsentrasi ekstrak bunga bugenvil 0%-100%. Setelah dilakukan uji MIC kemudian dilanjutkan pengujian efektivitas. Kedua uji ini menggunakan metode difusi kertas cakram. Parameter yang digunakan adalah diameter zona hambat yang terbentuk disekitar kertas cakram. Pembanding dalam penelitian ini adalah antijamur ketokonazol.
Hasil: Hasil pengujian MIC dan efektivitas didapatkan bahwa ekstrak bunga bugenvil memiliki kemampuan sebagai antijamur terhadap jamur Candida albicans. Dari analisis statistik pengujian Anova One-way pengaruh ekstrak bunga bugenvil dalam menghambat jamur Candida albicans memiliki nilai Fhitung>Ftabel dengan nilai α=0,05, dan kemudian diuji lanjut dengan menggunakan uji Duncan. Hasil uji Duncan menunjukan bahwa konsentrasi 70 % (A3) merupakan konsentrasi paling efektif dalam menghambat pertumbuhan jamur Candida albicans yang ditandai dengan hasil uji stastistik.
Kesimpulan: Penelitian ini menunjukkan ekstrak bunga bugenvil mempunyai aktivitas sebagai antijamur dengan konsentrasi 70% sebagai konsentrasi yang paling efektif dalam menghambat pertumbuhan jamur Candida albicans.
PENGARUH KUALITAS PELAYANAN TERHADAP KEPUASAN PASIEN RAWAT JALAN DI RSKDIA PERTIWI KOTA MAKASSAR
Authors: Mahfudah Ulfah , Dullah Arif Atul Mahmudah , Liliskarlina Liliskarlina , Asmaya Debi 
Published: March 2024

Kualitas pelayanan adalah upaya pemenuhan kebutuhan yang diiringi dengan keinginan konsumen serta ketepatan cara penyampaiannya agar dapat memenuhi harapan dan kepuasan pelanggan tersebut. Kepuasan pasien merupakan suatu tingkatan perasaan pasien yang timbul akibat dari kinerja layanan kesehatan yang diperolehnya setelah pasien membandingkan dengan apa yang diharapkannya.Metode penelitian yang digunakan adalah wawancara, dengan jenis penelitian kuantitatif dengan pendekatan cross sectional. Besar sampel 109 pasien di instalasi rawat jalan RSKDIA Pertiwi Kota Makassar pada bulan mei 2019. Penelitian ini menggunakan analisa chi-square dengan tingkat signifikan 0,05. Berdasarkan penelitian ini diperoleh variabel yang berhubungan dengan kualitas pelayanan terhadap kepuasan pasien di instalasi rawat jalan RSKDIA Pertiwi Makassar adalah bukti fisik (p=0,000), kehandalan (p=0,000), empati (p=0,000), jaminan (p=0,000), ketanggapan (p=0,000).Untuk meningkatkan kualitas pelayanan manajemen RSKDIA Pertiwi makassar harus meningkatkan keramahan kapada pasien, kecepat tanggapan, dan membuat evaluasi kepuasan pasien dengan cara mebuat kotak saran.
PENGARUH KONSUMSI KERUPUK SINGKONG EBI TERHADAP KUALITAS TIDUR PEKERJA WANITA DI PT MARUKI INTERNATIONAL INDONESIA
Authors: Upia Masydin , Baharuddin Alfina , Nurlinda Nurlinda 
Published: March 2024

Kualitas tidur seseorang dikatakan baik apabila tidak menunjukkan berbagai tanda kekurangan tidur dan tidak mengalami masalah dalam tidurnya. Buruknya kualitas tidur menyebabkan menurunnya konsentrasi dalam bekerja, menurunnya efesiensi dan produktifitas pekerjaan, human error (kesalahan dalam pelaporan tugas), tingkat absen meningkat, dan sering telat dalam menyelesaikan tugas. Didbutuhkan zat gizi yang optimal untuk mendukung produktifitas pekerja. Tujuan penelitian untuk menganalisis pengaruh pemberian keripik singkong ebi terhadap kebugaran dan kualitas tidur pada pekerja wanita. Penelitian ini menggunakan metode eksperimen sungguhan (True Experiment) dengan rancangan Randomized Control Trial Pretest-posttest with control group, yang bertujuan untuk melihat pengaruh yang timbul sebagai akibat dari adanya perlakuan tertentu. Populasi dalam penelitian ini adalah tenaga kerja wanita di Pt.Maruki International Indonesia tahun 2020, yang dipilih sesuai kriteria inklusi dan ekslusi. Metode analisis data menggunakan uji univariat (frequency distribution) dan uji bivariat (Wilcoxon). Hasil penelitian menunjukkan bahwa pada saat pre-test kategori baik sebanyak 1 pekerja (6.7%), kategori buruk sebanyak 14 pekerja (93,3%), sedangkan pada tahap post-test tingkat kualitas tidur pekerja wanita kategori baik sebanyak 0 mahasiswa (0%), kategori buruk sebanyak 15 mahasiswa (100%). Pada kelompok kontrol pada tahap pre-test tingkat kualitas tidur mahasiswa kategori baik sebanyak 0 pekerja (0%), kategori buruk sebanyak 15 pekerja (100%), sedangkan pada tahap post-test tingkat kualitas tidur pekerja wanita tidak berubah. Nilai p value 0.05 = 0.317 menunjukkan H0 ditolak dan Ha diterima, artinya pemberian keripik singkong ebi tidak berpengaruh terhadap kualitas tidur pada pekerja wanita.
Construction of an Escherichia coli chassis for efficient biosynthesis of human-like N-linked glycoproteins
Authors: Bao Zixin , Gao Yuting , Song Yitong , Ding Ning , Li Wei , Wu Qiong , Zhang Xiaomei , Zheng Yang , Li Junming , Hu Xuejun 
Published: March 2024

The production of N-linked glycoproteins in genetically engineered Escherichia coli holds significant potential for reducing costs, streamlining bioprocesses, and enhancing customization. However, the construction of a stable and low-cost microbial cell factory for the efficient production of humanized N-glycosylated recombinant proteins remains a formidable challenge. In this study, we developed a glyco-engineered E. coli chassis to produce N-glycosylated proteins with the human-like glycan Gal-β-1,4-GlcNAc-β-1,3-Gal-β-1,3-GlcNAc-, containing the human glycoform Gal-β-1,4-GlcNAc-β-1,3-. Our initial efforts were to replace various loci in the genome of the E. coli XL1-Blue strain with oligosaccharyltransferase PglB and the glycosyltransferases LsgCDEF to construct the E. coli chassis. In addition, we systematically optimized the promoter regions in the genome to regulate transcription levels. Subsequently, utilizing a plasmid carrying the target protein, we have successfully obtained N-glycosylated proteins with 100% tetrasaccharide modification at a yield of approximately 320 mg/L. Furthermore, we constructed the metabolic pathway for sialylation using a plasmid containing a dual-expression cassette of the target protein and CMP-sialic acid synthesis in the tetrasaccharide chassis cell, resulting in a 40% efficiency of terminal α-2,3- sialylation and a production of 65 mg/L of homogeneously sialylated glycoproteins in flasks. Our findings pave the way for further exploration of producing different linkages (α-2,3/α-2,6/α-2,8) of sialylated human-like N-glycoproteins in the periplasm of the plug-and-play E. coli chassis, laying a strong foundation for industrial-scale production.
Inhibition of malaria and babesiosis parasites by putative red blood cell targeting small molecules
Authors: Groomes Patrice V. , Paul Aditya S. , Duraisingh Manoj T. 
Published: March 2024

BackgroundChemotherapies for malaria and babesiosis frequently succumb to the emergence of pathogen-related drug-resistance. Host-targeted therapies are thought to be less susceptible to resistance but are seldom considered for treatment of these diseases.MethodsOur overall objective was to systematically assess small molecules for host cell-targeting activity to restrict proliferation of intracellular parasites. We carried out a literature survey to identify small molecules annotated for host factors implicated in Plasmodium falciparum infection. Alongside P. falciparum, we implemented in vitro parasite susceptibility assays also in the zoonotic parasite Plasmodium knowlesi and the veterinary parasite Babesia divergens. We additionally carried out assays to test directly for action on RBCs apart from the parasites. To distinguish specific host-targeting antiparasitic activity from erythrotoxicity, we measured phosphatidylserine exposure and hemolysis stimulated by small molecules in uninfected RBCs.ResultsWe identified diverse RBC target-annotated inhibitors with Plasmodium-specific, Babesia-specific, and broad-spectrum antiparasitic activity. The anticancer MEK-targeting drug trametinib is shown here to act with submicromolar activity to block proliferation of Plasmodium spp. in RBCs. Some inhibitors exhibit antimalarial activity with transient exposure to RBCs prior to infection with parasites, providing evidence for host-targeting activity distinct from direct inhibition of the parasite.ConclusionsWe report here characterization of small molecules for antiproliferative and host cell-targeting activity for malaria and babesiosis parasites. This resource is relevant for assessment of physiological RBC-parasite interactions and may inform drug development and repurposing efforts.
TRAIL and IP-10 dynamics in pregnant women post COVID-19 vaccination: associations with neutralizing antibody potency
Authors: Chen Wei-Chun , Hu Shu-Yu , Cheng Chao-Min , Shen Ching-Fen , Chuang Hui-Yu , Ker Chin-Ru , Sun Der-Ji , Shen Ching-Ju 
Published: March 2024

IntroductionThe aim of this study is to investigate changes in TNF-related apoptosis-inducing ligand (TRAIL) and gamma interferon-induced protein 10 (IP-10) after COVID-19 vaccination in pregnant women and to explore their association with neutralizing antibody (Nab) inhibition.MethodsThe study evaluated 93 pregnant women who had previously received two (n=21), three (n=55) or four (n=17) doses of COVID-19 vaccine. Also we evaluated maternal blood samples that were collected during childbirth. The levels of TRAIL, IP-10 and Nab inhibition were measured using enzyme-linked immunosorbent assays (ELISA).Results and discussionOur study revealed four-dose group resulted in lower TRAIL levels when compared to the two-dose and three-dose groups (4.78 vs. 16.07 vs. 21.61 pg/ml, p = 0.014). The two-dose group had reduced IP-10 levels than the three-dose cohort (111.49 vs. 147.89 pg/ml, p=0.013), with no significant variation compared to the four-dose group. In addition, the four-dose group showed stronger Nab inhibition against specific strains (BA.2 and BA.5) than the three-dose group. A positive correlation was observed between TRAIL and IP-10 in the two-dose group, while this relationship was not found in other dose groups or between TRAIL/IP-10 and Nab inhibition. As the doses of the COVID-19 vaccine increase, the levels of TRAIL and IP-10 generally increase, only by the fourth dose, the group previously vaccinated with AZD1222 showed lower TRAIL but higher IP-10. Despite these changes, more doses of the vaccine consistently reinforced Nab inhibition, apparently without any relation to TRAIL and IP-10 levels. The variation may indicate the induction of immunological memory in vaccinated mothers, which justifies further research in the future.
Diaphragm movement sensor for phrenic nerve monitoring during cryoballoon procedures: the first clinical evaluation
Authors: Schemoul Elsa , Tovmassian Lilith , Mancini Julien , Koutbi Linda , Biermé Cédric , Deharo Jean-Claude , Franceschi Frédéric , Maille Baptiste 
Published: March 2024

Background and aimsRight phrenic nerve palsy is the most frequent complication of cryoballoon procedures. The SMARTFREEZE™ console (Boston Scientific, St. Paul, MN, USA) has integrated a new tool for diaphragm monitoring—the Diaphragm Movement Sensor; however, it has not been evaluated in clinical practice. We aimed to assess the diagnostic performance of the Diaphragm Movement Sensor based on compound motor action potential data recorded simultaneously.MethodsThirty consecutive patients (mean age 63.2 ± 10.2 years) were included. We simultaneously recorded the compound motor action potential and the Diaphragm Movement Sensor during cryoapplications in the right pulmonary veins. The right phrenic nerve was paced at 60 per minute, 12 V and 2.9 ms. Compound motor action potential monitoring with a 30% decrease cutoff for the diagnosis of phrenic nerve threatening was considered the gold standard. The Diaphragm Movement Sensor decrease threshold was also set at 30%.ResultsConsidering compound motor action potential monitoring, phrenic nerve threatening occurred 11 times (in seven patients) among 84 cryoapplications (13.1%) at the right pulmonary veins. The sensitivity and specificity of the Diaphragm Movement Sensor were, respectively, 33% (95% CI: 7%–70%) and 49% (95% CI: 38%–61%; P &lt; 0.001). The predictive positive and negative values for the Diaphragm Movement Sensor were, respectively, 7% (95% CI: 2%–20%) and 86% (95% CI: 72%–95%). The Diaphragm Movement Sensor gave an erroneous diagnosis in 44/84 cryoapplications (52.4%).ConclusionsThe diagnostic performance of the Diaphragm Movement Sensor is low, and the relevance of its use in clinical practice may be debated.
The diagnostic role of resting myocardial blood flow in STEMI patients after revascularization
Authors: Yan Ming , Shang Hua , Guo Xiaorui , Hao Luping , Hou Shuang , Zheng Hongming 
Published: March 2024

BackgroundThe value of semiquantitative resting myocardial perfusion imaging (MPI) in coronary artery disease (CAD) is limited. At present, quantitative MPI can be performed by a new cadmium zinc tellurium single-photon emission computed tomography (CZT-SPECT) scan. The quantitative index of resting myocardial blood flow (MBF) has received little attention, and its manifestations and clinical value in the presence of unstable coronary blood flow have not been clarified.PurposeIn patients with ST-segment elevation myocardial infarction (STEMI), whether resting MBF can provide additional value of blood flow than semi-quantitative resting MPI is not sure. We also explored the influencing factors of resting MBF.MethodsThis was a retrospective clinical study. We included 75 patients with STEMI in the subacute phase who underwent resting MPI and dynamic scans after reperfusion therapy. General patient information, STEMI-related data, MPI, gated MPI (G-MPI), and resting MBF data were collected and recorded. According to the clinically provided culprit vessels, the resting MBF was divided into ischemic MBF and non-ischemic MBF. The paired Wilcoxon signed-rank test was used for resting MBF. The receiver operating characteristic (ROC) curves were used to determine the optimal threshold for ischemia, and multiple linear regression analysis was used to analyze the influencing factors of resting MBF.ResultsThere was a statistically significant difference between the ischemic MBF and non-ischemic MBF [0.59 (0.47–0.72) vs. 0.76 (0.64–0.93), p &lt; 0.0001]. The ROC curve analysis revealed that resting MBF could identify ischemia to a certain extent, with a cutoff value of 0.5975, area under the curve (AUC) = 0.666, sensitivity = 55.8%, and specificity = 68.7%. Male sex and summed rest score (SRS) were influencing factors for resting MBF.ConclusionTo a certain extent, resting MBF can suggest residual ischemia after reperfusion therapy in patients with STEMI. There was a negative correlation between male sex, SRS, and ischemic MBF. A lower resting MBF may be associated with more severe myocardial ischemia.
Beyond (COVID-19) lockdown: faculty experiences in the post-pandemic academic landscape
Authors: Dewangan Roshan Lal , Longkumer Imlisongla , Gupta Shashidhar , Pathak Smriti 
Published: March 2024

BackgroundThis study investigates the nuanced experiences of faculty members in higher education institutions during and after the COVID-19 pandemic. Focusing on family–work conflict, job satisfaction, and personal wellbeing, the research aims to provide comprehensive insights into the challenges and adaptations encountered by faculty members amidst unprecedented disruptions.MethodA mixed-method approach was employed, encompassing both quantitative and qualitative measures. The quantitative facet involved 82 participants who responded surveys distributed to faculty members across diverse regions of India. Concurrently, qualitative data were collected through interviews with 30 faculty members in three states. The quantitative study utilized standardized tools, while the qualitative inquiry followed a semi-structured interview schedule.ResultQuantitative findings revealed a significant upswing in job satisfaction after institutional reopening compared to the lockdown period. However, no significant differences were observed concerning work–family conflict and personal wellbeing. Notably, faculty members reported heightened work–family and family–work interference compared to national statistics. Qualitative responses highlight a notable shift in teaching methodologies, incorporating multimedia and online tools. Faculty members exhibited mixed sentiments about returning to the office, expressed a deepened appreciation for social relationships post-reopening, and emphasized the positive impact of institutional hygiene protocols.ConclusionThis study offers crucial insights into the multifaceted experiences of faculty members in higher institutions during the COVID-19 lockdown and subsequent reopening. The research contributes valuable perspectives to the evolving discourse on post-pandemic academia, providing a foundation for further exploration and understanding of the challenges and opportunities faced by faculty members in the changing scenario of higher education.
Characterization of the m6A regulators’ landscape highlights the clinical significance of acute myocardial infarction
Authors: Chao Peng , Zhang Xueqin , Zhang Lei , Wang Yong , Wusiman Miriban , Aimaijiang Gulizere , Chen Xiaoyang , Yang Yining 
Published: March 2024

ObjectiveAcute myocardial infarction (AMI) is a severe cardiovascular disease that threatens human life and health globally. N6-methyladenosine (m6A) governs the fate of RNAs via m6A regulators. Nevertheless, how m6A regulators affect AMI remains to be deciphered. To solve this issue, an integrative analysis of m6A regulators in AMI was conducted.MethodsWe acquired transcriptome profiles (GSE59867, GSE48060) of peripheral blood samples from AMI patients and healthy controls. Key m6A regulators were used for LASSO, and consensus clustering was conducted. Next, the m6A score was also computed. Immune cell infiltration, ferroptosis, and oxidative stress were evaluated. In-vitro and in-vivo experiments were conducted to verify the role of the m6A regulator ALKBH5 in AMI.ResultsMost m6A regulators presented notable expression alterations in circulating cells of AMI patients versus those of controls. Based on key m6A regulators, we established a gene signature and a nomogram for AMI diagnosis and risk prediction. AMI patients were classified into three m6A clusters or gene clusters, respectively, and each cluster possessed the unique properties of m6A modification, immune cell infiltration, ferroptosis, and oxidative stress. Finally, the m6A score was utilized to quantify m6A modification patterns. Therapeutic targeting of ALKBH5 greatly alleviated apoptosis and intracellular ROS in H/R-induced H9C2 cells and NRCMs.ConclusionAltogether, our findings highlight the clinical significance of m6A regulators in the diagnosis and risk prediction of AMI and indicate the critical roles of m6A modification in the regulation of immune cell infiltration, ferroptosis, and oxidative stress.