bluuebunny/crossref_metadata_2025_split huggingface dataset:
Les fractures de l’extrémité supérieure du fémur : Estimation du coût médical direct de la prise en charge hospitalière
Authors: Dorgham Sana , Selmene Mohamed Amine , Zaraa Mourad , Lahmar Ahmed Amine , Annabi Hedi , Mahjoub Sabri 
Published: February 2024

Introduction : Les fractures de l’extrémité supérieure du fémur (ESF) constituent un problème de santé publique du fait de leur prévalence élevée, leur morbi-mortalité et leurs lourdes conséquences socio-économiques.
Objectif : Estimer le coût médical direct de la prise en charge hospitalière des fractures de l’ESF.
Méthodes: Il s’agissait d’une étude descriptive prospective étalée sur six mois à propos de patients hospitalisés et opérés pour une fracture de l’ESF. Afin d’estimer le coût nous nous sommes référés à la nomenclature des actes professionnels et les tarifs fixés par le ministère de la santé publique et le ministère des Finances.
Résultats : Nous avons recensé 100 patients. Cinquante-cinq des patients étaient des femmes. L’âge moyen était de 76 ans. La durée moyenne du séjour hospitalier était de cinq jours. Le délai moyen préopératoire était de trois jours. Le coût total moyen de la prise en charge de ces fractures était estimé à 2877 Dinars Tunisiens. Les dépenses se répartissaient en : frais d’hôtelleries et coût des ressources humaines 40%, coût de l’implant 25,9%, coût des consommables 12%, coût opératoire 10%, coût des examens complémentaires 9% et coût des médicaments 2%.
Conclusion : Les fractures de l'ESF ont des conséquences financières majeures sur les budgets. Pour diminuer le coût de sa prise en charge hospitalièrelimiter au maximum la durée du séjour hospitalier, s’investir dans les prothèses totale et intermédiaire de hanche et adopter un comportement économique en tenant compte du coût des consommables.
L’instrumentalisation des principes de « démocratie de participation » chez les élus québécois du début de la Révolution tranquille
Authors: Savard Stéphane 
Published: February 2024

Les années 1960 sont connues comme une période de contestations politiques, sociales et culturelles. Les penseurs et les mouvements sociaux associés à la Nouvelle Gauche formulent de nombreuses idées, dont celle de la décolonisation, et revendiquent de nouveaux droits. Plusieurs en viennent à concevoir une nouvelle forme de démocratie, la « démocratie de participation » ou « démocratie participative », dont les principes et les valeurs occupent alors une place importante dans les discours et les stratégies militantes des mouvements sociaux. Cet article ne s’intéresse pas aux discours de ces derniers ; il cherche plutôt à déterminer comment la notion de démocratie participative, qui circule dans l’espace public, est récupérée par les responsables politiques d’une société, en l’occurrence les élus de l’Assemblée législative du Québec. En analysant les discours des élus québécois entre 1960 et 1968 (gouvernements Lesage et Johnson), il étudie les premières allusions directes ou indirectes à la question de la « démocratie de participation » et cherche à comprendre les raisons qui sous-tendent l’utilisation de cette expression ou les revendications de ses principes. L’article montre que les élus québécois instrumentalisent les principes à la base de la « démocratie de participation », afin de justifier leurs points de vue et leurs orientations en matière d’éducation et de planification économique et régionale. Ils font de même pour promouvoir, de manière rhétorique ou concrète, une réforme de la gouvernance et de la démocratie parlementaire.
Les politiques et les dispositifs qui façonnent l’expérience des élèves immigrants et de leurs familles en contexte minoritaire francophone ontarien
Authors: Fleuret Carole , Dulude Éliane 
Published: February 2024

L’école, comme lieu de socialisation, n’est pas culturellement neutre, car elle reproduit les valeurs et les modes de pensée privilégiés des classes dominantes. D’une part, ces valeurs et ces modes de pensée sont incités par des politiques éducatives quant à la participation parentale. D’autre part, les dispositifs promus auprès des écoles, pour favoriser l’implantation de ces politiques et de ces pratiques collaboratives visant à réduire les décalages entre celles de l’école et celles des familles immigrantes, semblent essentiels. Nous proposerons, dans un premier temps, d’effectuer une analyse documentaire qualitative des politiques éducatives en Ontario afin de dégager les valeurs et les normes véhiculées, susceptibles d’influencer la mise en oeuvre de ces collaborations (ex. : stratégie d’équité et d’éducation inclusive, cadre d’efficacité). Dans un deuxième temps, nous discuterons de la manière dont ces valeurs et ces normes sont susceptibles de façonner la mise en oeuvre de dispositifs qui reconnaissent l’hétérogénéité et qui visent à réduire les écarts entre les élèves au regard de leur habitus familial et culturel.
Co-differential genes between DKD and aging: implications for a diagnostic model of DKD
Authors: Du Hongxuan , He Kaiying , Zhao Jing , You Qicai , Zhou Xiaochun , Wang Jianqin 
Published: February 2024

Objective
Diabetic kidney disease (DKD) is a serious complication of diabetes mellitus (DM) that is closely related to aging. In this study, we found co-differential genes between DKD and aging and established a diagnostic model of DKD based on these genes.

Methods
Differentially expressed genes (DEGs) in DKD were screened using GEO datasets. The intersection of the DEGs of DKD and aging-related genes revealed DKD and aging co-differential genes. Based on this, a genetic diagnostic model for DKD was constructed using LASSO regression. The characteristics of these genes were investigated using consensus clustering, WGCNA, functional enrichment, and immune cell infiltration. Finally, the expression of diagnostic model genes was analyzed using single-cell RNA sequencing (scRNA-seq) in DKD mice (model constructed by streptozotocin (STZ) injection and confirmed by tissue section staining).

Results
First, there were 159 common differential genes between DKD and aging, 15 of which were significant. These co-differential genes were involved in stress, glucolipid metabolism, and immunological functions. Second, a genetic diagnostic model (including IGF1, CETP, PCK1, FOS, and HSPA1A) was developed based on these genes. Validation of these model genes in scRNA-seq data revealed statistically significant variations in FOS, HSPA1A, and PCK1 gene expression between the early DKD and control groups. Validation of these model genes in the kidneys of DKD mice revealed that Igf1, Fos, Pck1, and Hspa1a had lower expression in DKD mice, with Igf1 expression being statistically significant.

Conclusion
Our findings suggest that DKD and aging co-differential genes are significant in DKD diagnosis, providing a theoretical basis for novel research directions on DKD.
Gap and proposals for educational practices of sexual and reproductive health for the LGBTQIA+ population: an integrative review / Lacunas e propostas de práticas educativas de saúde sexual e reprodutiva para população LGBTQIA+: revisão integrativa
Authors: Nepomuceno de Paiva Carla Cardi , Bastos Mariana Guimarães 
Published: February 2024

Objetivo: analisar as publicações científicas nacionais sobre a atenção à saúde sexual e reprodutiva oferecida para população LGBTQIA+ com relação às principais lacunas e possibilidades das práticas educativas referentes a essa temática no contexto brasileiro. Método: revisão integrativa, em seis etapas, com busca dos estudos realizada em junho de 2022 nas bases de dados LILACS, MEDLINE, SCIELO e Web of Science e SCOPUS. Resultados: foram recuperados 5.643 estudos e selecionados 14 para análise e discussão. Destacaram-se os temas: saúde sexual e reprodutiva com foco na reprodução e nas técnicas reprodutivas, infecções sexualmente transmissíveis e educação em saúde sexual e, por fim, direitos sexuais e reprodutivos. Conclusão: foram evidenciadas as principais lacunas quanto ao tema de saúde sexual e reprodutiva, assim como sugestões para práticas educativas ligadas à temática.
Developmental regulation of
DNA
methyltransferases
Authors: Lees‐Murdock Diane J. , Walsh Colum P. 
Published: April 2006

Abstract
The mammalian genome appears to undergo two waves of demethylation, one in the preimplantation embryo and the other in the germ cells of the primitive gonad. Methylation is reestablished at the egg cylinder stage and either in the prospermatogonia for the male or the growing oocyte for the female. Precise control of methyltransferase activity is required to ensure that these events occur reliably. Here we discuss the various mechanisms, from promoter switching to protein stabilization, used to regulate the maintenance and de novo enzymes catalyzing CpG methylation during development, and how these converge to limit the periods during which all the required factors are present in the nucleus.
The Use of Substituted Iridium Complexes in Doped Polymer Electrophosphorescent Devices: The Influence of Triplet Transfer and Other Factors on Enhancing Device Performance
Authors: King S. M. , Al‐Attar H. A. , Evans R. J. , Congreve A. , Beeby A. , Monkman A. P. 
Published: April 2006

AbstractThe problem of phosphorescence quenching by the host polymer of a dopant in a polyfluorene‐based electrophosphorescent device has been extensively studied. This paper concentrates on reduction of the rate of triplet‐energy transfer from the dopant to the host by making inert t‐butyl substitutions to the ligands of the well‐understood fac‐trisphenylpyridine iridium phosphorescent dopant. These substitutions introduce steric bulk to the dopant that approximately halves the rate of energy transfer compared to the unsubstituted dopant, and a concomitant increase in device performance is observed. This is attributed to the strong distance dependence of the Dexter‐type energy transfer involved, the steric bulk of the t‐butyl groups effectively preventing the energy transfer from emissive dopant to the host. In addition, through the use of specific substitutions on either the pyridyl or phenyl ring, the pathway of the energy transfer has been identified as being through the pyridyl ring of the ligand. Employing this technique of steric prevention of the triplet‐energy transfer to the host reduces the need for development of hosts with a high triplet level for electrophosphorescent devices.
Cyclophilin A is overexpressed in human pancreatic cancer cells and stimulates cell proliferation through CD147
Authors: Li Min , Zhai Qihui , Bharadwaj Uddalak , Wang Hao , Li Fei , Fisher William E. , Chen Changyi , Yao Qizhi 
Published: April 2006

AbstractBACKGROUNDAlthough overexpression of cyclophilin A (CypA) is associated with several types of cancer, its role in pancreatic cancer has not been studied. In this study the expression of CypA and its receptor CD147 on pancreatic cancer was determined as well as the effect of exogenous CypA on pancreatic cancer cell proliferation.METHODSThe expression of CypA and CD147 in human pancreatic cancer cell lines and tissues was determined with real‐time reverse transcriptase polymerase chain reaction (RT‐PCR), Western blot, and immunostaining. Cell proliferation in response to CypA was performed by [3H]thymidine incorporation assay. Phosphorylation of MAPK and cytokine secretion profiles in pancreatic cancer cells were determined by using the Bio‐Plex phosphoprotein assay and cytokine assay.RESULTSPancreatic cancer cell lines expressed significantly higher levels of CypA and CD147 than normal human pancreatic ductal epithelium (HPDE) cells. Expression of CypA and CD147 was also substantially higher in human pancreatic adenocarcinoma tissues than those in normal pancreatic tissues. Addition of exogenous CypA significantly stimulated pancreatic cancer cell proliferation in a dose‐dependent manner and this effect was effectively blocked by pretreatment with anti‐CD147 antibody. In addition, CypA activated ERK1/2 and p38 MAPK signaling pathways and increased the secretion of 2 key cytokines IL‐5 and IL‐17 in Panc‐1 cells.CONCLUSIONSThe expression of CypA and CD147 was significantly increased in both pancreatic cancer cell lines and tissues. Exogenous CypA promotes pancreatic cancer cell growth, which may be mediated through the interaction with CD147 and the activation of ERK1/2 and p38 MAPKs. Cancer 2006. © 2006 American Cancer Society.
Multiplex analysis of circulating cytokines in the sera of patients with different clinical forms of visceral leishmaniasis
Authors: Kurkjian Katie M. , Mahmutovic Aida J. , Kellar Kathryn L. , Haque Rashidul , Bern Caryn , Secor W. Evan 
Published: April 2006

AbstractBackground:The clinical spectrum of visceral leishmaniasis (VL), a chronic intracellular parasitic disease, ranges from a subclinical, asymptomatic infection to severe clinical disease (kala‐azar). In experimental leishmaniasis, mice that have a Th1 response to infection tend to have limited disease while a Th2 response is associated with disease progression. Humans with VL most often have mixed rather than polarized responses. However, most clinical studies have used methods that require a relatively large sample volume, thus limiting their scope. Measuring multiple cytokine levels in blood samples using a multiplexed microsphere assay (MMA) may be useful to further evaluate the Th1/Th2 paradigm in humans.Methods:Bangladeshi individuals (n = 120) living in an area endemic for VL were categorized into one of the five clinical categories. Sera from these individuals were measured for levels of IL‐2, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, IL‐12, IFN‐γ, and TNF‐α by multiplexed microsphere cytokine immunoassay.Results:Circulating IL‐8, IL‐10, and IL‐12 differed significantly among the clinical groups. Persons with kala‐azar demonstrated the highest median levels of IL‐8 and IL‐10 but lower median levels of IL‐12.Conclusions:The MMA for cytokines is an extremely time‐and sample‐efficient method for characterizing circulating cytokine levels in visceral leishmaniasis patients. © 2006 International Society for Analytical Cytology
Effect of microsphere binding site density on the apparent affinity of an interaction partner
Authors: Iannone Marie A. , Consler Thomas G. 
Published: April 2006

AbstractBackground:Flow cytometric microsphere‐based binding assays can be used to measure molecular interactions with high sensitivity. We have used multiplexed microsphere technology to explore the effect that binding site density has on the apparent affinity of a soluble interaction partner.Methods:The interaction of a nuclear receptor, peroxisome proliferator‐activated receptor gamma ligand binding domain (PPARγ LBD), with a synthetic peptide derived from a nuclear receptor coactivator protein, PPARγ coactivator‐1 alpha (PGC‐1α), is the interacting system being studied. The density of this peptide coupled to fluorescently unique microsphere populations is varied by co‐incubating the biotinylated peptide and avidin‐coated microsphere populations with increasing the amounts of free D‐biotin. The discrete‐density peptide‐coupled microsphere populations are combined to conduct a multiplexed binding experiment with Alexa 532‐labeled PPARγ LBD, in the absence or presence of a small molecule ligand.Results:As the immobilized binding site density of PGC‐1α peptide on fluorescent microspheres is increased the measured apparent affinity for PPARγ LBD is increased.Conclusions:The density of binding sites immobilized to a surface has a pronounced effect on the apparent affinity for soluble binding partners. By controlling and varying the binding site density it is possible to increase the sensitivity of an interaction assay. In multiplexed assay formats it should be possible to normalize intrinsically unequal binding interactions by individually optimizing the binding site density of the immobilized interaction partner. However, to quantitatively measure intrinsic affinities of molecular interactions, low binding site densities are required and multivalent reagents must be avoided. © 2006 International Society for Analytical Cytology
Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP™ technology
Authors: Johannisson Anders , Jonasson Robert , Dernfalk Johanna , Jensen‐Waern Marianne 
Published: April 2006

AbstractBackground:Multiplex flow cytometry is in widespread use for detection of cytokines in human samples. However, no report on the measurement of porcine cytokines using this method has previously been published. We report on the detection of the porcine proinflammatory cytokines TNF‐α, IL‐8, and IL‐1β by the xMap‐assay for multiplex flow cytometry.Methods:Commercially available antibodies to porcine cytokines were used as capture antibodies by attaching them to goat anti‐mouse IgG coated microspheres with different fluorescent signatures. By the use of biotinylated detection antibodies and SAv‐PE the amount of cytokines bound to the spheres were measured. Experiments were performed to determine the limits of detection and the amount of crossreactivity in buffer, serum, and plasma, using spiking with recombinant porcine cytokines.Results:The limit of detection ranged from 0.18 to 12 ng/ml. Generally, the detection limit was higher in serum and plasma, than in buffer. No crossreactivity between reagents was found.Conclusions:Porcine proinflammatory cytokines can be detected utilizing this method with satisfactory detection limits, and no crossreaction between the reagents involved. © 2006 International Society for Analytical Cytology
Amiodarone lung toxicity in a cardiac transplant candidate initially diagnosed by fine‐needle aspiration: Cytologic, histologic, and electronmicroscopic findings
Authors: Omeroglu Gulbeyaz , Kalugina Yelena , Ersahin Cagatay , Wojcik Eva M. 
Published: April 2006

AbstractA cardiac transplant candidate with ischemic cardiomyopathy developed bilateral small parenchymal opacities in lower lobes of the lung. A fine‐needle aspiration (FNA) was performed that revealed changes characteristic of amiodarone toxicity. Subsequently performed lung biopsies and electron microscopic studies confirmed the initial FNA diagnosis. The patient has been successfully transplanted with marked improvement in his clinical findings. This is the first case of amiodarone lung toxicity where the diagnosis was initially suggested based on the FNA findings. We also describe the clinical, cytological, histological, and electron microscopic (EM) findings of amiodarone‐related pulmonary toxicity and provide a review of the literature. Diagn. Cytopathol. 2006;34:351–354. © 2006 Wiley‐Liss, Inc.
Dysbacteriosis and squamous (pre)neoplasia of immigrants and Dutch women as established in population‐based cervical screening
Authors: Verbruggen Banut‐Sabine M. , Boon Mathilde E. , Boon L. Matthijs 
Published: April 2006

AbstractWe examined the statistical relationships between dysbacteriosis and (pre)neoplasia related to age and ethnicity from the cervical screening of almost half a million smears. Data from 445,080 smears were coded according to KOPAC (the Dutch national cervical smear coding system) with nine grades. Prevalence per 100,000 smears and relative risks (RR) were calculated for dysbacteriosis and for squamous abnormalities. Patients were stratified by their probable country of origin. Dutch women had an RR of 0.92 for dysbacteriosis. Surinamese women had the highest RR for dysbacteriosis (RR = 2.36) and Moroccan women had the lowest (RR = 1.00). The same trends were seen for the risks of squamous abnormalities. The data for Turkish women follow the patterns of those for Surinamese women. The RR of dysbacteriosis is highest at 50 yr (1.28) and lowest at 35 yr (0.86). When dysbacteriotic and non‐dysbacteriotic smears were compared, dysbacteriosis was observed more frequently in smears with squamous abnormalities (4.1% vs. 2.2%). Dysbacteriosis may warrant more intensive cytological surveillance and changes in lifestyle. Diagn. Cytopathol. 2006;34: 377–381. © 2006 Wiley‐Liss, Inc.
Can hamartoma of the breast be distinguished from fibroadenoma using fine‐needle aspiration cytology?
Authors: Herbert Mehrdad , Mendlovic Sonia , Liokumovich Pavel , Segal Michael , Zahavi Sergei , Rath‐Wolfson Lea , Sandbank Judith 
Published: April 2006

AbstractIn an attempt to determine if it is possible to distinguish hamartoma of the breast from fibroadenoma using fine‐needle aspiration cytology, we reviewed the cytological slides of 13 histopathologically confirmed cases of hamartoma of the breast and compared them with the cytological features of 13 histologically confirmed fibroadenomas. In each case, we studied the epithelial and stromal features. Cytologic characteristics were retrospectively evaluated in a semiquantitive manner. In conclusion, the finding of intact lobular units and a relative paucity of stroma may suggest the diagnosis of hamartoma. Diagn. Cytopathol. 2006;34:326–329. © 2006 Wiley‐Liss, Inc.
Cytological features of prostatic intraepithelial neoplasia
Authors: Valdman Alexander , Jonmarker Sara , Ekman Peter , Egevad Lars 
Published: April 2006

AbstractFine‐needle aspiration cytolotogy (FNAC) is an acknowledged method for diagnosing prostate cancer. False‐positive results are uncommon, but concerns have been raised that prostatic intraepithelial neoplasia (PIN) could be misinterpreted as carcinoma. Therefore, we attempted to describe cytological features of PIN. Cells were scraped from macroscopically normal areas of 177 radical prostatectomy specimens, smeared and Giemsa‐stained. Histological slides from these areas were reviewed, and 17 samples with high‐grade PIN and with no invasive cancer were selected. Smears from 17 invasive cancers were used for comparison. Cancer showed high cellularity and dissociation, while PIN smears only contained a few clusters of atypical cells. Pronounced nuclear atypia, prominent or multiple nucleoli and mucin were more common in cancer, while cytoplasmic granules, crystalloids and cluster size did not distinguish between PIN and cancer. In conclusion, PIN should not be diagnosed by FNAC alone. However, a highly cellular smear with pronounced atypia seems to preclude PIN. Diagn. Cytopathol. 2006;34:317–322. © 2006 Wiley‐Liss, Inc.
Cytology of pancreatic acinar cell carcinoma
Authors: Stelow Edward B. , Bardales Ricardo H. , Shami Vanessa M. , Woon Carolyn , Presley Allison , Mallery Shawn , Lai Rebecca , Stanley Michael W. 
Published: April 2006

AbstractAcinar cell carcinoma (ACC) of the pancreas is extremely uncommon and its cytologic features have rarely been described. We describe the cytologic features of cases we have seen, review the literature regarding its cytologic features and discuss the pitfalls that may be encountered and the use of immunohistochemistry for its diagnosis. We searched our databases for all cases of histologically confirmed pancreatic ACC which had undergone prior fine needle aspiration (FNA) of the primary pancreatic lesion. The clinical histories, radiographic and sonographic findings, cytologic features, original cytologic diagnoses, and final histologic diagnoses were reviewed. Four cases of pancreatic ACC were found that had undergone FNA prior to histologic confirmation of the diagnoses. They were from 2 men and 2 women aged 50–75 yr. All masses were in the head of the pancreas, 2 had apparent peri‐pancreatic adenopathy and 1 had an apparent liver metastasis. On review, all 4 had had diagnostic material on cytology samples. Original cytologic diagnoses included “acinar cell carcinoma,” “pancreatic endocrine tumor,” “favor neuroendocrine tumor, low‐grade” and “non‐diagnostic specimen.” The cytologic features included small to moderate‐sized loose groups with numerous single cells, prominent acinar formation, little anisonucleosis and prominent nucleoli. The cytologic features showed significant overlap with those of pancreatic endocrine tumors. Diagn. Cytopathol. 2006; 34:367–372. © 2006 Wiley‐Liss, Inc.