bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Term Follow-Up of Patients with Non-Hodgkin Lymphoma Following Myeloablative Therapy and Autologous Transplantation of CD34+-Selected Peripheral Blood Progenitor Cells
Authors: Witzens-Harig Mathias , Heilmann Conny , Hensel Manfred , Kornacker Martin , Benner Axel , Haas Rainer , Fruehauf Stefan , Ho Anthony D. 
Published: January 2007

Abstract
Graft engineering by CD34+ selection of peripheral blood progenitor cells (PBPC) has been used in non-Hodgkin lymphoma (NHL) with the aim to reduce relapse related to tumor cells within the graft. From September 1995 to January 2000, 39 patients with newly diagnosed (n = 31) or relapsed (n = 8) NHL were treated in our institution with myeloablative therapy followed by CD34+ selected autologous PBPC transplantation. Thirty-one patients were diagnosed with follicular lymphoma, and eight patients with mantle-cell lymphoma. All patients had advanced disease (26% of patients stage III and 74% stage IV, Ann Arbor classification). Induction therapy resulted in a complete remission in 17 patients and a partial remission in 22 patients. PBPC were mobilized after cytotoxic chemotherapy with granulocyte colony-stimulating factor support. CD34+ selection was performed using immunomagnetic beads (Baxter Isolex 300SA or 300i Magnetic Cell Separation System). Most patients (85%) received total body irradiation and high-dose cyclophosphamide as myeloablative regimen. Twelve patients also received rituximab 375 mg/m2 before radiation and before the start of the cyclophosphamide treatment. The mean CD34+ cell number for transplantation was 6.5 × 106 CD34+ cells/kg of body weight. Platelet recovery (&gt;20,000/μl median on day 13) and leukocyte recovery (&gt;1,000/μl median on day 12) were within expected range. The estimated median follow-up was 47 months. The probabilities of freedom from progression, overall survival, and event-free survival 4 years after transplantation were 96%, 90%, and 87%, respectively, for patients with follicular lymphoma and 42%, 63%, and 33%, respectively, for patients with mantle-cell lymphoma. Risk factors for relapse were age and extranodal manifestation of disease. The rate of lethal infections in the 12-month follow-up period was 8%. We conclude that CD34+ selection of autologous transplants following myeloablative therapy is feasible and results in long-term remission in the majority of patients, but the procedure is probably related to a higher rate of lethal infections.
Comparison theorems for noncanonical third order nonlinear differential equations
Authors: Mojsej Ivan , Ohriska Ján 
Published: January 2007

Abstract
The aim of our paper is to study oscillatory and asymptotic properties of solutions of nonlinear differential equations of the third order with quasiderivatives. We prove comparison theorems on property A between linear and nonlinear equations. Some integral criteria ensuring property A for nonlinear equations are also given. Our assumptions on the nonlinearity of f are restricted to its behavior only in a neighborhood of zero and a neighborhood of infinity.
Effect of dimethylmaleate and phorone on glutathione content of Setaria cervi (Nematoda: Filarioidea) during in vitro incubation
Authors: Gupta S. , Banu N. , Srivastava A. 
Published: January 2007

Abstract
Glutathione metabolism represents a prospective target for antifilarial drug design, and therefore, the alterations in glutathione (GSH) content of filarial worms by known mammalian GSH depletors i.e. dimethylmaleate (DMM) and phorone were first thought for investigation in model filarial worms Setaria cervi. The dose dependent GSH depletion was achieved when these worms were incubated at 37°C for 6 h in Hanks balanced salt solution with varying concentrations (10–250 μM) of DMM or phorone. During the short incubation period of 6 h, 250 μM of DMM and phorone declined more than 90 % of the GSH content of filarial worms.
Accountable Care Organization Leader Perspectives on the Medicare Shared Savings Program
Authors: Khullar Dhruv , Schpero William L. , Casalino Lawrence P. , Pierre Reekarl , Carter Samuel , Civelek Yasin , Zhang Manyao , Bond Amelia M. 
Published: March 2024

ImportanceThe Medicare Shared Savings Program (MSSP) includes more than 400 accountable care organizations (ACOs) and is among the largest and longest running value-based payment efforts in the US. However, given recent program reforms and other changes in the health care system, the experiences and perspectives of ACO leaders remain incompletely characterized.ObjectiveTo understand the priorities, strategies, and challenges of ACO leaders in MSSP.Design, Setting, and ParticipantsIn this qualitative study, interviews were conducted with leaders of 49 ACOs of differing sizes, leadership structures, and geographies from MSSP between September 29 and December 29, 2022. Participants were asked about their clinical and care management efforts; how they engaged frontline clinicians; the process by which they distributed shared savings and added or removed practices; and other factors that they believed influenced their success or failure in the program.Main Outcomes and MeasuresLeader perspectives on major themes related to ACO initiatives, performance improvement, and the recruitment, engagement, and retention of clinicians.ResultsOf the 49 ACOs interviewed, 34 were hospital-associated ACOs (69%), 35 were medium or large (&amp;gt;10 000 attributed beneficiaries) (71%), and 17 were rural (35%). The ACOs had a mean (SD) tenure of 8.1 (2.1) years in MSSP. Five major themes emerged: (1) ACO leaders reported a focus on annual wellness visits, coding practices, and care transitions; (2) leaders used both relationship-based and metrics-based strategies to promote clinician engagement; (3) ACOs generally distributed half or more of shared savings to participating practices; (4) ACO recruitment and retention efforts were increasingly influenced by market competition; and (5) some hospital-associated ACOs faced misaligned incentives.Conclusions and RelevanceIn this study, the ACO leaders reported varied approaches to promoting clinician alignment with ACO goals, an emphasis on increasing annual wellness visits, and new pressures related to growth of other care models. Policymakers hoping to modify or expand the program may wish to incorporate these perspectives into future reforms.
Recognition of Genetic Conditions After Learning With Images Created Using Generative Artificial Intelligence
Authors: Waikel Rebekah L. , Othman Amna A. , Patel Tanviben , Ledgister Hanchard Suzanna , Hu Ping , Tekendo-Ngongang Cedrik , Duong Dat , Solomon Benjamin D. 
Published: March 2024

ImportanceThe lack of standardized genetics training in pediatrics residencies, along with a shortage of medical geneticists, necessitates innovative educational approaches.ObjectiveTo compare pediatric resident recognition of Kabuki syndrome (KS) and Noonan syndrome (NS) after 1 of 4 educational interventions, including generative artificial intelligence (AI) methods.Design, Setting, and ParticipantsThis comparative effectiveness study used generative AI to create images of children with KS and NS. From October 1, 2022, to February 28, 2023, US pediatric residents were provided images through a web-based survey to assess whether these images helped them recognize genetic conditions.InterventionsParticipants categorized 20 images after exposure to 1 of 4 educational interventions (text-only descriptions, real images, and 2 types of images created by generative AI).Main Outcomes and MeasuresAssociations between educational interventions with accuracy and self-reported confidence.ResultsOf 2515 contacted pediatric residents, 106 and 102 completed the KS and NS surveys, respectively. For KS, the sensitivity of text description was 48.5% (128 of 264), which was not significantly different from random guessing (odds ratio [OR], 0.94; 95% CI, 0.69-1.29; P = .71). Sensitivity was thus compared for real images vs random guessing (60.3% [188 of 312]; OR, 1.52; 95% CI, 1.15-2.00; P = .003) and 2 types of generative AI images vs random guessing (57.0% [212 of 372]; OR, 1.32; 95% CI, 1.04-1.69; P = .02 and 59.6% [193 of 324]; OR, 1.47; 95% CI, 1.12-1.94; P = .006) (denominators differ according to survey responses). The sensitivity of the NS text-only description was 65.3% (196 of 300). Compared with text-only, the sensitivity of the real images was 74.3% (205 of 276; OR, 1.53; 95% CI, 1.08-2.18; P = .02), and the sensitivity of the 2 types of images created by generative AI was 68.0% (204 of 300; OR, 1.13; 95% CI, 0.77-1.66; P = .54) and 71.0% (247 of 328; OR, 1.30; 95% CI, 0.92-1.83; P = .14). For specificity, no intervention was statistically different from text only. After the interventions, the number of participants who reported being unsure about important diagnostic facial features decreased from 56 (52.8%) to 5 (7.6%) for KS (P &amp;lt; .001) and 25 (24.5%) to 4 (4.7%) for NS (P &amp;lt; .001). There was a significant association between confidence level and sensitivity for real and generated images.Conclusions and RelevanceIn this study, real and generated images helped participants recognize KS and NS; real images appeared most helpful. Generated images were noninferior to real images and could serve an adjunctive role, particularly for rare conditions.
Dual Functional Microcapsule based on Monodisperse Short PEG Amphiphile for Drug Encapsulation and Protein Affinity Controlled Release
Authors: Ketkar Rohit N. , Dey Paritosh , Sodnawar Triveni , Sharma Shilpy , M Manikandan , Dutta Choudhury Sharmistha , Sadhukhan Nabanita 
Published: March 2024

AbstractA short monodisperse poly(ethylene glycol) (PEG) and a neutral organic rotamer conjugate TEG‐BTA‐2 amphiphile was designed for the construction of a stimuli‐responsive switchable self‐assembled structure for drug encapsulation by noncovalent interaction and targeted controlled delivery. A short PEG, tetraethylene glycol (TEG) was covalently attached with a neutral organic rotamer benzothiazole dye (BTA‐2) affording the neutral TEG‐BTA‐2 (<500 D). The TEG‐BTA‐2 is self‐assembled into a microsphere in an aqueous medium, but remarkably undergoes morphology change switching to a rice‐like microcapsule for curcumin encapsulation. Curcumin‐loaded microcapsules were stable in an aqueous solution, however, were noticed disintegrating upon the addition of BSA protein. This is possibly due to an interaction with BSA protein leading to a protein affinity‐controlled curcumin release in a neutral PBS buffer. Moreover, cell internalization of the neutral amphiphile TEG‐BTA‐2 into A549 cells was observed by fluorescence microscopy, providing an opportunity for application as a molecular vehicle for targeted drug delivery and monitoring.
Resolvins protect against diabetes‐induced colonic oxidative stress, barrier dysfunction, and associated diarrhea via the HO‐1 pathway
Authors: Yu Ting , Chen Die , Qi Hongyan , Lin Lin , Tang Yurong 
Published: March 2024

AbstractDiabetes is associated with increased oxidative stress, leading to altered tight junction formation and increased apoptosis in colonic epithelial cells. These changes may lead to intestinal barrier dysfunction and corresponding gastrointestinal symptoms in patients with diabetes, including diarrhea. The aim of this study was to characterize the effect and mechanism of Resolvin D1 (RvD1) on diabetes‐induced oxidative stress and barrier disruption in the colon. Mice with streptozotocin‐induced diabetes were treated with RvD1 for 2 weeks, then evaluated for stool frequency, stool water content, gut permeability, and colonic transepithelial electrical resistance as well as production of reactive oxygen species (ROS), apoptosis, and expression of tight junction proteins Zonula Occludens 1 (ZO‐1) and occludin. The same parameters were assessed in human colonoid cultures subjected to elevated glucose. We found that RvD1 treatment did not affect blood glucose, but normalized stool water content and prevented intestinal barrier dysfunction, epithelial oxidative stress, and apoptosis. RvD1 also restored ZO‐1 and occludin expression in diabetic mice. RvD1 treatment increased phosphorylation of Akt and was accompanied by a 3.5‐fold increase in heme oxygenase‐1 (HO‐1) expression in the epithelial cells. The protective effects of RvD1 were blocked by ZnPP, a competitive inhibitor of HO‐1. Similar findings were observed in RvD1‐treated human colonoid cultures subjected to elevated glucose. In conclusion, Oxidative stress in diabetes results in mucosal barrier dysfunction, contributing to the development of diabetic diarrhea. Resolvins prevent ROS‐mediated mucosal injury and protect gut barrier function by intracellular PI3K/Akt activation and subsequent HO‐1 upregulation in intestinal epithelial cells. These actions result in normalizing stool frequency and stool water content in diabetic mice, suggesting that resolvins may be useful in the treatment of diabetic diarrhea.
Tolerability of a piezoelectric microneedle electroporator in human subjects
Authors: Lu Chao‐Yi , Rohilla Pankaj , Felner Eric I. , Byagathvalli Gaurav , Azizoglu Erkan , Bhamla M. Saad , Prausnitz Mark R. 
Published: March 2024

AbstractElectroporation, or the use of electric pulses to facilitate the intracellular delivery of DNA, RNA, and other molecules, is a well‐established technique, that has been demonstrated to significantly augment the immunogenicity of DNA/mRNA vaccines and therapeutics. However, the clinical translation of traditional electroporators has been limited due to high costs, large size, complex user operation, and poor tolerability in humans due to nerve stimulation. In prior work, we introduced ePatch: an ultra‐low‐cost, handheld, battery‐free electroporator employing a piezoelectric pulser coupled with a microneedle electrode array that showed enhanced immunogenic responses to an intradermal SARS‐CoV‐2 DNA vaccine in mice. The current study shifts focus from efficacy to tolerability, hypothesizing that ePatch's microneedle array, which localizes the electric field to the superficial skin strata, will minimize nerve stimulation and improve patient comfort. We tested this hypothesis in 14 healthy adults, monitoring pain and other potential adverse effects associated with electroporation. Compared to the insertion of a traditional hypodermic needle, the ePatch was less painful. Adverse effects such as pain, tenderness, erythema and swelling at the application sites were minimal, transient, and statistically indistinguishable between the experimental and placebo ePatch application, suggesting excellent tolerability towards electroporation. In summary, ePatch has a favorable tolerability profile in humans and offers the potential for the safe use of electroporation in a variety of clinical settings, including DNA and mRNA vaccination.
Fabrication of biomimetic scaffold through hybrid forming technique
Authors: Celik Dilek , Ustundag Cem Bulent 
Published: March 2024

AbstractBone tissue engineering procedures require the use of a scaffold with a sufficiently porous structure, which serves as a three‐dimensional template for cell attachment, followed by tissue regeneration and vascularization both in vitro and in vivo. This study aimed to create scaffold material with a unique hybrid forming that met these parameters. Slip casting and freeze‐drying processes were used with a hybrid forming approach to create a bone scaffold based on hydroxyapatite (HA) mimicking the structure of cortical and cancellous bones, respectively. HA was synthesized by the wet chemical precipitation method. Fourier‐transform infrared spectroscopy, dynamic light scattering, X‐ray diffraction, thermogravimetric analysis‐differential thermal analysis, Brunauer‐Emmett‐Teller, He Pycnometer, and scanning electron microscope analyses were performed to characterize the scaffold. According to analysis results, HA particle size was found at 137.8 nm. The optimum sintering temperature was determined to be 1300°C. The specific surface area of the HA powder was measured as 55.11 m2/g. The total open porosity of the hybrid scaffold was calculated as 70%. Scaffold successfully substituted both cancellous and cortical layers of bone regarding structural characteristics, porosity, and mechanical strength. Considering morphological characteristics, a hybrid scaffold might facilitate vascularization, osteoinduction, and osteoconduction. Research findings suggest that the hybrid design strongly resembled natural bone and is suitable for both load‐bearing and non‐bearing bones.
In Situ Detection of Nucleic Acids in Extracellular Vesicles via Membrane Fusion
Authors: Feng Jianzhou , Gong Yanli , Li Qianqian , Yang Chaoyong , An Yu , Wu Lingling 
Published: March 2024

AbstractExtracellular vesicles (EVs) carry diverse biomolecules (e. g., nucleic acids, proteins) for intercellular communication, serving as important markers for diseases. Analyzing nucleic acids derived from EVs enables non‐invasive disease diagnosis and prognosis evaluation. Membrane fusion, a fundamental cellular process wherein two lipid membranes merge, facilitates cell communication and cargo transport. Building on this natural phenomenon, recent years have witnessed the emergence of membrane fusion‐based strategies for the detection of nucleic acids within EVs. These strategies entail the encapsulation of detection probes within either artificial or natural vesicles, followed by the induction of membrane fusion with EVs to deliver probes. This innovative approach not only enables in situ detection of nucleic acids within EVs but also ensures the maintenance of structural integrity of EVs, thus preventing nucleic acid degradation and minimizing the interference from free nucleic acids. This concept categorizes approaches into universal and targeted membrane fusion strategies, and discusses their application potential, and challenges and future prospects.
Estimating multivariate ecological variables at high spatial resolution using a cost‐effective matching algorithm
Authors: Renne Rachel R. , Schlaepfer Daniel R. , Palmquist Kyle A. , Lauenroth William K. , Bradford John B. 
Published: March 2024

AbstractSimulation models are valuable tools for estimating ecosystem response to environmental conditions and are particularly relevant for investigating climate change impacts. However, because of high computational requirements, models are often applied over a coarse grid of points or for representative locations. Spatial interpolation of model output can be necessary to guide decision‐making, yet interpolation is not straightforward because the interpolated values must maintain the covariance structure among variables. We present methods for two key steps for utilizing limited simulations to generate detailed maps of multivariate and time series output. First, we present a method to select an optimal set of simulation sites that maximize the area represented for a given number of sites. Then, we introduce a multivariate matching approach to interpolate simulation results to detailed maps for the represented area. This approach links simulation output to environmentally analogous matched sites according to user‐defined criteria. We demonstrate the methods with case studies using output from (1) an individual‐based plant simulation model to illustrate site selection, and (2) an ecosystem water balance simulation model to illustrate interpolation. For the site selection case study, we identified 200 simulation sites that represented 96% of a large study area (1.12 × 106 km2) at a ~1‐km resolution. For the interpolation case study, we generated ~1‐km resolution maps across 4.38 × 106 km2 of drylands in North America from a 10 × 10 km grid of simulated sites. Estimates of interpolation errors using cross validation were low (<10% of the range of each variable). Our point selection and interpolation methods, which are available as an easy‐to‐use R package, provide a means of cost‐effectively generating detailed maps of expensive, complex simulation output (e.g., multivariate and time series) at scales relevant for local conservation planning. Our methods are flexible and allow the user to identify relevant matching criteria to balance interpolation uncertainty with areal coverage to enhance inference and decision‐making at management‐relevant scales across large areas.
Sediment particle tracking data for the Carpathian reservoir under climate and land use change scenarios
Authors: Szalińska Ewa , Hachaj Paweł S. , Szlapa Monika , Orlińska‐Woźniak Paulina , Wilk Paweł 
Published: March 2024

AbstractAlthough the Carpathian Mts. area is considered as extremely prone to surface erosion which results in capacity loss of the dammed reservoirs, a lack of data to follow details of this process is perceivable. The research of the selected sediment fractions transport tracking was conducted using the capabilities of the digital platform—Macromodel DNS (Discharge‐Nutrient‐Sea) for the catchment with drinking water reservoir in the Polish part of Western Carpathian. The continuity of sediment transport simulation in two hydrologically different elements of the catchment—the river and the reservoir—was possible due to consolidation of two models in the platform—SWAT (Soil & Water Assessment Tool) and AdH/PTM (Adaptive Hydraulics Model/Particle Tracking Model). The result of those modules' integration was a database for tracking the individual sediment fractions delivered to the reservoir and deposited in specific reservoir zones. The implementation of climate and land use change scenarios allowed additionally to analyse the estimation of those processes in the future. The simulation outcomes consist of daily flows and monthly sediment loads at the reservoir inflow and the individual sediment particle fractions deposition location inside of the reservoir.
Global Chert Database: A summary record of global chert samples
Authors: Wang Chenyu , Zhong Hanting , Yan Han , Gao Lingxue , Huang Hu , Hou Mingcai 
Published: March 2024

AbstractChert is a sedimentary rock abundant and conspicuous throughout the geologic record. It serves as an essential geological archive and is vital for palaeogeographic reconstruction. The Chert Database Working Group is part of the OneSediment Working Groups of the Deep‐time Digital Earth (DDE) Big Science Program. To facilitate the sharing of chert information and promote chert research, we have compiled a summary of literature containing chert information worldwide and established the Global Chert Database (GCDB). The main body of the current database consists of seven data tables, each providing details on references, lithology, depositional age, geographic location, depositional environment and geochemical information for each sample. As of December 2023, the GCDB contains 8417 sample data from 617 pieces of literature, which can be downloaded from the ‘DDE Data Publish & Repository’.