bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-cell sequencing of PBMC characterizes the transformation of T cell subsets in the inflammatory microenvironment of RA-ILD
Authors: Zhang Yuan , Zhu Jie , Xiao Kang , Liu Hongya , Du Kui , Wu Dandan , Zou Qinghua 
Published: February 2024

Abstract
Rheumatoid arthritis-interstitial lung disease (RA-ILD), associated with Rheumatoid Arthritis (RA), typically manifests as usual interstitial pneumonia (UIP) and non-specific interstitial pneumonia (NSIP). However, the pathogenic mechanisms underlying RA and its related ILD are not fully understood. The progression of single-cell sequencing technology has enhanced our comprehension of the inflammatory environment, offering potential insights into inflammation-related cells. In this study, we conducted single-cell sequencing and cell communication analysis on peripheral blood mononuclear cells (PBMCs) from patients with RA, RA + UIP, and RA + NSIP. Our results revealed that blood cells from patients were annotated as T cells, NK cells, neutrophils, red blood cells, B cells, monocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells, indicating the accumulation of T cells in the inflammatory environment. Temporal analysis suggested a differentiation trend in T cells: CD4 + T cells, regulatory T cells, CD69 + T cells, and CD8 + T cells. Subgroup enrichment analysis of T cells identified significantly differentially expressed genes (DEGs) enriched in pathways such as rheumatoid arthritis, IL-17 signaling, Th1 and Th2 cell differentiation, Th17 cell differentiation, and TNF signaling. Further investigation revealed the pro-inflammatory characteristics of this subgroup, and cell communication results indicated a pivotal role of T cells. In summary, our comprehensive analysis identified T cells as crucial mediators in RA-ILD and unveiled potential pathways, including interactions among HLA, MIF, CLEC2C ligands, and CD8A, CD8B, CD74 receptors. These findings provided potential biomarkers for effective treatment targets and intervention strategies, deepening our understanding of T cell participation in the immune pathogenesis of RA-ILD.
Effectiveness of Strategy-Focused Training in Colorectal Endoscopic Submucosal Dissection: A Retrospective Observational Study
Authors: Chiba Hideyuki , Ohata Ken , Ashikari Keiichi , Tachikawa Jun , Okada Naoya , Hayashi Akimichi , Ebisawa Yu , Kobayashi Mikio , Arimoto Jun , Kuwabara Hiroki , Nakaoka Michiko 
Published: February 2024

Abstract

Background
Colorectal ESD, an advanced minimally invasive treatment, presents technical challenges, with globally varying training methods. We analyzed the learning curve of ESD training, emphasizing preoperative strategies, notably gravity traction, to guide ESD instructors and trainee programs.
Method
This retrospective study included 881 cases guided by an experienced supervisor. Six trainees received “strategy-focused” instruction. To evaluate the number of ESD experiences in steps, the following phases were classified based on ESD experiences of each trainees: Phase 0 (0–50 ESD), Phase 1 (51–100 ESD), Phase 2 (101–150 ESD), and Phase 3 (151–200 ESD). Lesion background, outcomes, and safety were compared across phases. Factors contributing to technical difficulty in early (Phase 0 and 1) and late phases (Phase 2 and 3) were identified, along with the utility of traction ESD with device assistance.
Result
Treatment outcomes were favorable, with 99.8% and 94.7% en bloc resection and curative resection rates, respectively. Approximately 90% self-completion rate could be achieved after experiencing about 50 cases (92.7% in Phase 1), signifying proficiency growth despite increased case difficulty. In early phases, factors such as right-sided colon, LST-NG morphology, and mild and severe fibrosis pose challenges. In late phases, mild and severe fibrosis remained significant. Traction-assisted ESD, utilized in 3% of cases, comprised planned (1.1%) and rescue (1.9%) methods. Planned traction aided specific lesions, while rescue traction was common in the right colon.
Conclusion
"Strategy-focused" ESD training consistently yields successful outcomes, effectively adapting to varying difficulty factors in different proficient stages.
A novel strategy to facilitate uniform epithelial cell maturation using liquid–liquid interfaces
Authors: Sonoi Rie , Kamihira Masamichi 
Published: February 2024

Abstract
Epithelial tissue forms and maintains a critical barrier function in the body. A novel culture design aimed at promoting uniform maturation of epithelial cells using liquid materials is described. Culturing Madin–Darby canine kidney (MDCK) cells at the liquid–liquid interface yielded reduced migration and stimulated active cell growth. Similar to solid–liquid interfaces, cells cultured on a fibronectin-coated liquid–liquid interface exhibited active migration and growth, ultimately reaching a confluent state. These cells exhibited reduced stress fiber formation and adopted a cobblestone-like shape, which led to their even distribution in the culture vessel. To inhibit stress fiber formation and apoptosis, the exposure of cells on liquid–liquid interfaces to Y27632, a specific inhibitor of the Rho-associated protein kinase (ROCK), facilitated tight junction formation (frequency of ZO-2-positive cells, FZ = 0.73). In Y27632-exposed cells on the liquid–liquid interface, the value obtained by subtracting the standard deviation of the ratio of nucleus densities in each region that compartmentalized a culture vessel from 1, denoted as HLN, was 0.93 ± 0.01, indicated even cell distribution in the culture vessel at t = 72 h. The behavior of epithelial cells on liquid–liquid interfaces contributes to the promotion of their uniform maturation.
Delineating the Therapeutic Potential of Topical Insulin for Ocular Disease Management: A Systematic Review and Meta-Analysis
Authors: Andrade Luís Jesuino de Oliveira , de Oliveira Gabriela Correia Matos , França Caroline Santos , Bittencourt Alcina Maria Vinhaes , Nascimento Guilherme Peixoto , Silva Catharina Peixoto , de Oliveira Luís Matos 
Published: February 2024

Abstract
Background: Ocular surface disorders (OSD) are highly prevalent, affecting millions globally. While conventional treatments offer some level of success, they often fall short in addressing challenging refractory cases. This unmet need has spurred the investigation of novel therapeutic approaches, including the exploration of topical insulin as a potential alternative.
Objective: This systematic review and meta-analysis evaluated the safety and efficacy of topical insulin for OSD management.
Methods: We conducted a comprehensive search across major databases (PubMed, Scopus, Embase, and Cochrane Library) for studies investigating the use of topical insulin for ocular conditions. Studies were rigorously selected based on pre-defined inclusion/exclusion criteria and assessed for methodological quality using established tools. We extracted and analyzed data pertaining to safety, efficacy, and relevant outcomes for meta-analysis, including healing time.
Results: Ten studies (diverse designs: 1 case-control, 3 randomized prospective, 3 retrospective, and 3 double-blind) met the inclusion criteria. These studies explored a range of OSD, including neurotrophic corneal ulcers, persistent epithelial defects, recurrent epithelial erosions, dry eye disease, and postoperative corneal wound healing in diabetic patients. The findings suggest encouraging outcomes for topical insulin across these indications, demonstrating its potential to OSD. A meta-analysis comparing healing times between topical insulin and placebo revealed a statistically significant advantage for the insulin group (Q = 26.81, df = 3, P = 0.000, I² = 96%).
Conclusion: This comprehensive review and meta-analysis provide promising evidence for the therapeutic potential of topical insulin in diverse OSD. While methodological limitations exist, particularly in non-randomized studies, the current body of evidence suggests that topical insulin may offer a safe and effective treatment option for patients with refractory OSD.
Survey of Economically Important Flora of District Bahawalnagar for Sustainable Harvesting
Authors: Ghufran Asad , Khan Muhammad Bilal , Anwar Muhammad 
Published: February 2024

Abstract
An ethnobotanical survey of medicinal and other economically important flora used by native people in Bahawalnagar was carried out from April 2020 to December 2020. To compile and document medicinally and economically important plants in accordance with traditional practices in the area, the aim of this study was to conduct ethnobotanical investigations in the area. A total of 54 informants, including 9 hakims (herbal doctors) and 3 pansars (medicinal plant traders), were randomly selected from the study sites. Ethnobotanical data were collected using semistructured questionnaires, field observations, interviews and group discussions. During the course of the study, 58 plant species belonging to 32 families were collected in the study area. Cucurbitaceae has 6 plant species, and Fabaceae and Poaceae have 4 plant species. The most frequently used plant parts were the leaves (39%), followed by fruits (43%). The most widely treated diseases were abdominal problems followed by general body pain. In the study area, agricultural expansion, firewood collection, timber production, and construction pose major threats to plants in general and medicinal plants in particular. The cultivation of medicinal plants is not common in the region. The management and conservation of medicinal plants has been made possible in part by indigenous practices, diverse cultural customs, and seasonally restricted plant collection. As a result, people should be given the opportunity to grow medicinal plants at home in their gardens. The local people should be encouraged to be involved in the sustainable utilization and management of plant resources, as well as creating awareness by educating them on how to do so.
Analysis of scaling relationships for flood parameters and peak discharge estimation in a tropical region
Authors: Mazivanhanga Charles , Grabowski Robert C. , Pérez-Sánchez Eunice , Carballo-Cruz Victor R. 
Published: February 2024

Abstract
Relationships between peak discharges and catchment size (e.g., flood scaling) in a catchment have the potential to support new river flood forecasting approaches but have not been tested in tropical regions. This study determined flood scaling relationships between peak discharge and nested drainage areas in the La Sierra catchment (Mexico). A statistical power law equation was applied to selected rainfall–runoff events that occurred between 2012 and 2015. Variations in flood scaling parameters were determined in relation to catchment descriptors and processes for peak downstream discharge estimation. Similar to studies in humid temperate regions, the results reveal the existence of log-linear relationships between the intercept (α) and exponent (θ) parameter values and the log–log power–law relationships between (α) and the peak discharge observed from the smallest headwater catchments. The flood parameter values obtained were then factored into the scaling equation (QP = αAθ) and successfully predicted downstream flood peaks, especially highly recurrent flood events. The findings contribute to a better understanding of the nature of flood wave generation and support the development of new flood forecasting approaches in unregulated catchments suitable for non-stationarity in hydrological processes with climate change.
La afiliación a la seguridad social del trabajo no remunerado del hogar: el modelo de Ecuador como ejemplo para un debate necesario
Authors: Guamán Hernández Adoración , Lorente Campos Raúl 
Published: February 2024

La inclusión en el sistema de seguridad social de una protecciónespecífica para las personas que realizan el trabajo no remuneradodel hogar vio la luz en Ecuador con la Ley para la Justicia Laboral yReconocimiento del Trabajo en el Hogar, del 20 de abril de 2015, quereformó la Ley de Seguridad Social. La ley de 2015 responde al mandatoexpreso en la Constitución ecuatoriana de 2008 de inserción en la seguridadsocial de las personas que realizan trabajo no remunerado en los hogares.
Argumentación jurídica del derecho humano a la vivienda en México
Authors: Medina Conde Ana Laura , Flores Ilhuicatzi Uziel 
Published: February 2024

El presente trabajo tiene como objetivo general analizar, pormedio de la argumentación jurídica, el derecho humano a la vivienda enMéxico, que tiene como fundamento el artículo 11 del Pacto Internacionalde Derechos Económicos, Sociales y Culturales, el artículo 123 constitucional y el artículo 3 de la Ley del Instituto del Fondo Nacional dela Vivienda para los Trabajadores (Infonavit), al establecer que se debeotorgar crédito “barato” a los trabajadores, se observa Atendiendo a Ferrajoli, se observa que a nivel factual el crédito otorgado a los trabajadores no es menor que el otorgado por las instituciones bancarias, es igual o superior; se utiliza el modelo de Toulmin y las categorías de argumentos de MacCormick como relación entre las premisas y la conclusión
Strategy (Business) Using Instagram Social Media @Ruang_Youthforia
Authors: Mukhlish Muhammad Maududi  , Nugroho Arif Widodo , Syaharani Vina Aulia , Mardiah Hanifah 
Published: February 2024

This research is intended to find out and obtain an overview of the strategies created by ruang_youthforia to increase the number of followers who become segmentation or target market who will become users of services or products from ruang_youthforia. This type of research is a type of case study research, using a qualitative approach with the constructivism paradigm to obtain in-depth results based on the real conditions and situations of what is on the ground. Data collection techniques that researchers use by conducting observations, interviews, and documentation. Research conducts data analysis by examining data obtained from observations, interviews, and documentation. To test the validity of the data, researchers conducted by comparing, unifying, and combining all the data that has been obtained results from this study, formulating whether ruang_youthforia has carried out marketing strategies to increase the number of followers that will be analyzed using integrated marketing communication strategies that include Promotion (promotion), Public Relations (Public Relations), Advertising, Direct Marketing, Personal selling, Publicity, Event, Interactive Marketing to communicate its vision and mission,
Pulse-wave analysis during routine blood pressure measurement shows potential to distinguish between sinus and nonsinus rhythm
Authors: Yang Chung-Chun , Chan I-Ping , Hsueh Tung , Lin Shiun-Long 
Published: February 2024

Abstract

OBJECTIVE
To investigate whether pulse-wave analysis (PWA) performed by trained evaluators facilitates detection of nonsinus rhythm.

ANIMALS
Same-day, high-definition oscillometry pulse-wave data and ECG results of 155 animals (144 dogs and 11 cats) were analyzed.

METHODS
In this cross-sectional study, we enrolled 18 participants from various backgrounds, all of whom received PWA training. The ability to distinguish between sinus and nonsinus rhythms was assessed using receiver operating characteristic curve analysis. The pulse-wave datasets were divided into 5 ECG categories. Agreement between ECG diagnoses and PWA-based arrhythmia detection was evaluated using Cohen κ values, and the correlation between the academic year of veterinary students and their κ values was assessed.

RESULTS
All cardiology researchers demonstrated satisfactory accuracy in distinguishing pathological rhythms using PWA (area under the curve, 0.704 to 0.761), with the highest accuracy in detecting atrial fibrillation (area under the curve, 0.811 to 0.845). Fair agreement with ECG categorization was achieved by all 3 cardiology researchers, 2 of 5 general practitioners, and 3 of 10 veterinary undergraduates. The veterinary undergraduates' years of study were correlated with their diagnostic performance (Spearman ρ = 0.658; P = .019).

CLINICAL RELEVANCE
PWA during routine noninvasive blood pressure measurement showed significant potential for the detection of pathological arrhythmias, notably atrial fibrillation. This approach yielded improved effectiveness when it was used by veterinarians with cardiology experience. Thus, introducing hands-on training courses, particularly those focused on cardiology and interactive workshops, may enable frontline veterinarians to promptly identify arrhythmias using PWA, facilitating timely ECG examinations or referrals.
Intranasal booster vaccination of beef steers reduces clinical signs following experimental coinfection with BRSV and BHV-1 without reducing shedding of BRD-associated bacteria
Authors: Martínez David A. , Chamorro Manuel F. , Passler Thomas , Huber Laura , Falkenberg Shollie , Walz Paul H. , Thoresen Merrilee , Raithel Gage , Silvis Scott , Dimitrov Kiril M. , Stockler Ricardo , Woolums Amelia R. 
Published: February 2024

Abstract

OBJECTIVE
To determine the efficacy of primary or booster intranasal vaccination of beef steers on clinical protection and pathogen detection following simultaneous challenge with bovine respiratory syncytial virus and bovine herpes virus 1.

METHODS
30 beef steers were randomly allocated to 3 different treatment groups starting at 2 months of age. Group A (n = 10) was administered a single dose of a parenteral modified-live vaccine and was moved to a separate pasture. Groups B (n = 10) and C (10) remained unvaccinated. At 6 months of age, all steers were weaned and transported. Subsequently, groups A and B received a single dose of an intranasal modified-live vaccine vaccine while group C remained unvaccinated. Group C was housed separately until challenge. Two days following vaccination, all steers were challenged with bovine respiratory syncytial virus and bovine herpes virus 1 and housed in a single pen. Clinical and antibody response outcomes and the presence of nasal pathogens were evaluated.

RESULTS
The odds of clinical disease were lower in group A compared with group C on day 7 postchallenge; however, antibody responses and pathogen detection were not significantly different between groups before and following viral challenge. All calves remained negative for Histophilus somni and Mycoplasma bovis; however, significantly greater loads of Mannheimia haemolytica and Pasteurella multocida were detected on day 7 postchallenge compared with day −2 prechallenge.

CLINICAL RELEVANCE
Intranasal booster vaccination of beef steers at 6 months of age reduced clinical disease early after viral challenge. Weaning, transport, and viral infection promoted increased detection rates of M haemolytica and P multocida regardless of vaccination status.
The Impact of Artificial Intelligence Banking and Personal Interaction Quality towards Customer Retention with Customer Satisfaction as an Intervening Variable
Authors: Chintasi Angreani  , Nur Afifah  , Barkah  
Published: February 2024

Number of digital banking transactions Indonesia based on katadata.com in August 2023 grew by 169,9% throughout the last five years in five years and the amount of third-party banking funds in Indonesia increased by 12.3% according to Indonesian Banking Statistics data for 2020-2022. Number of digital transactions at Bank Maybank Indonesia in 2022 increased by 28% and the number of accounts increased in 2022 by 34%, however the amount of funds raised by Bank Maybank Indonesia decreased by 1.19%. It is alleged that the acquisition of accounts is not followed by customers who retain. This study was conducted to explore the effect of artificial intelligence banking and personal interaction quality on customer retention with customer satisfaction as an intervening variable. The research sample was 215 customer respondents using questionnaires and interviews. The analysis method uses Structural Equation Models Partical Least Square (SEMPLS) based on Smart Partial Least Square (Smart PLS) 3.2.9. The results of the study are artificial intelligence banking has a significant effect on customer retention, personal intelligence banking does not have a significant effect on customer retention. When mediated by customer satisfaction, artificial intelligence banking still has a significant effect on customer retention and personal intelligence banking has a significant effect on customer retention through customer satisfaction as an intervening variable.
The correlation of folic acid and homocysteine serum with vascular density and retinal non-perfusion area in diabetic retinopathy
Authors: Guna Dharma Andhika , Wildan Arief , Maharani Maharani , Prihatningtias Riski , Luthfia Rahmi Fifin , Nugroho Trilaksana , Novitasari Saubig Arnila , Rikiandraswida Zahira 
Published: February 2024

Background : Diabetic Retinopathy (DR) is the most common microvascular complication of Diabetes Mellitus (DM). Homocysteine has been studied as a biomarker in DR, while folic acid exhibits anti-proliferative effects in DR.
Objective : To analyze the correlation between folic acid and homocysteine serum with vascular density and retinal non-perfusion area in healthy individuals and patients with diabetic retinopathy.
Methods : This is an observational study with a cross-sectional design, conducted in Dr. Kariadi Hospital and GAKI laboratory in Semarang in January 2023. This study included 60 samples: 15 healthy individuals, 15 patients with DM but no DR, 15 patients with Non-Proliferative Diabetic Retinopathy (NPDR), and 15 patients with Proliferative Diabetic Retinopathy (PDR). Patients were examined for serum folic acid and homocysteine using blood laboratory tests, vessel density and retinal non-perfusion areas using optical coherence tomography angiography (OCTA).
Results : There was a negative correlation with weak strength between folic acid levels and retinal non-perfusion area of the retina in all samples (Folic acid levels vs retinal non-perfusion area, p = 0.009, Spearman correlation = -0.335). There was a positive correlation with weak strength between folic acid levels and vascular density in all samples (Folic acid levels vs vascular density, p = 0.009, Spearman correlation = 0.337). There was a positive correlation with moderate strength between homocysteine levels and retinal non-perfusion area in all samples (Homocysteine levels vs non–perfusion area of the retina, p = 0.001, Spearman correlation = 0.426). There was a positive correlation with moderate strength between homocysteine levels and vascular density in all samples (Homocysteine levels vs vascular density, p = 0.001, Spearman correlation = -0.414).
Conclusion : There was a correlation between folic acid and homocysteine serum with vascular density and retinal non-perfusion areas.
Conhecimentos e vivências de mulheres sobre contracepção
Authors: Borba Keylla Gonzales , Prates Lisie Alende , Gomes Natália da Silva , Gomes Bruna Cristiane Furtado , Cherubim Daiani Oliveira , Lipinski Jussara Mendes 
Published: February 2024

Objetivo: Analisar os conhecimentos e vivências de mulheres sobre contracepção. Métodos: Trata-se de uma pesquisa qualitativa, descritiva e exploratória, desenvolvida entre os meses de julho e outubro de 2022, com 16 mulheres, em uma Estratégia de Saúde da Família, localizada na Fronteira Oeste do Rio Grande do Sul. Utilizou-se entrevista semiestruturada e análise de conteúdo temática. Resultados: As mulheres apresentam conhecimento limitado sobre o mecanismo de ação dos métodos contraceptivos, bem como sobre a sua disponibilidade no Sistema Único de Saúde. A maioria demonstrou preferência pelos métodos hormonais, apresentando receio e desconhecimento sobre outras opções. A maioria acredita que a contracepção é responsabilidade do casal. Conclusão: Conclui-se que é preciso ofertar espaços para a troca de conhecimentos sobre assuntos ligados ao planejamento reprodutivo, contribuindo para a garantia dos direitos sexuais e reprodutivos. Ainda, considera-se fundamental a necessidade de responsabilidade compartilhada acerca dos métodos contraceptivos pelos casais e maior empoderamento feminino.