bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a Hydrophobic Domain of HA2 Essential to Morphogenesis of
Helicoverpa armigera
Nucleopolyhedrovirus
Authors: Wang Qian , Wang Yun , Liang Changyong , Song Jianhua , Chen Xinwen 
Published: January 2008

ABSTRACT

The HA2 protein of the
Helicoverpa armigera
single-nucleocapsid nucleopolyhedrovirus (HearNPV) is a WASP homology protein capable of nucleating branched actin filaments in the presence of the Arp2/3 complex in vitro. To determine the role of
ha2
in the HearNPV life cycle,
ha2
knockout and
ha2
repair bacmids were constructed. Transfection and infection analysis demonstrated that the
ha2
null bacmid was unable to produce infectious budded virus (BV), while the repair bacmid rescued the defect. In vitro analysis demonstrated that the WCA domain of HA2 accelerates Arp2/3-mediated actin assembly and is indispensable to the function of HA2. However, analysis of the repaired recombinant with a series of truncated
ha2
mutants demonstrated that the WCA domain was essential but not enough to yield infectious virions, and a hydrophobic domain (H domain) consisting of amino acids (aa) 167 to 193 played a pivotal role in the production of BV. Subcellular localization analysis with enhanced green fluorescent protein fusions showed that the H domain functioned as a nuclear localization signal. In addition, deletion of the C terminus of the
ha2
product, a phosphatidylinositol 4-kinase homolog, dramatically decreased the viral titer, while deletion of 128 aa from the N terminus did not affect HA2 function.
Selective Ablation of Virion Host Shutoff Protein RNase Activity Attenuates Herpes Simplex Virus 2 in Mice
Authors: Korom Maria , Wylie Kristine M. , Morrison Lynda A. 
Published: January 2008

ABSTRACT

The virion host shutoff (vhs) protein of herpes simplex virus (HSV) has endoribonuclease activity and rapidly reduces protein synthesis in infected cells through mRNA degradation. Herpes simplex virus 1 (HSV-1) and HSV-2 vhs mutants are highly attenuated in vivo, but replication and virulence are largely restored to HSV-2 vhs mutants in the absence of a type I interferon (IFN) response. The role of vhs in pathogenesis and the hindrance of the type I IFN response have classically been examined with viruses that completely lack vhs or express a truncated vhs protein. To determine whether RNase activity is the principal mechanism of vhs-mediated type I IFN resistance and virulence, we constructed a HSV-2 point mutant that synthesizes full-length vhs protein lacking RNase activity (RNase
−
virus). Wild-type and mutant HSV-2 vhs proteins coimmunoprecipitated with VP16 and VP22. vhs protein bearing the point mutation was packaged into the virion as efficiently as the wild-type vhs protein. Like a mutant encoding truncated vhs, the RNase
−
virus showed IFN-dependent replication that was restricted compared with that of the wild-type virus. The RNase
−
virus was highly attenuated in wild-type mice infected intravaginally, with reduced mucosal replication, disease severity, and spread to the nervous system comparable to those of the vhs truncation mutant. Surprisingly, in alpha/beta interferon (IFN-α/β) receptor knockout mice, the vhs RNase mutant was more attenuated than the vhs truncation mutant in terms of disease severity and virus titer in vaginal swabs and central nervous system samples, suggesting that non-enzymatically active vhs protein interferes with efficient virus replication. Our results indicate that vhs enzymatic activity plays a complex role in vhs-mediated type I IFN resistance during HSV-2 infection.
Potent Human Immunodeficiency Virus-Neutralizing and Complement Lysis Activities of Antibodies Are Not Obligatorily Linked
Authors: Huber Michael , von Wyl Viktor , Ammann Christoph G. , Kuster Herbert , Stiegler Gabriela , Katinger Hermann , Weber Rainer , Fischer Marek , Stoiber Heribert , Günthard Huldrych F. , Trkola Alexandra 
Published: January 2008

ABSTRACTTo evaluate the contribution of complement-mediated lysis to the in vivo activities of neutralizing antibodies, we analyzed the influence of complement activation on treatment success in a recent passive immunization trial with the neutralizing monoclonal antibodies 2G12, 2F5, and 4E10. Administration of monoclonal antibodies led to an immediate, high activation of the complement system even in the absence of viremia in the 14 participating human immunodeficiency virus-infected individuals. Lysis activity measured in patient plasma increased during passive immunization; however, the increases were modest and only partially attributable to the administration of antibodies. We found that unlike neutralization activity, lysis activity was not associated with treatment success in this trial. Compared to complement lysis mounted by the polyclonal antibody response in vivo, monoclonal antibodies were weak inducers of this activity, suggesting that polyclonal responses are more effective in reaching the required threshold of complement activation. Importantly, strong neutralization activity of the monoclonal antibodies did not predict complement lysis activity against patient and reference viruses, suggesting that these activities are not linked. In summary, our data support the notion that the in vivo activities of 2G12, 2F5, and 4E10 are likely due to direct neutralization or Fc receptor-mediated mechanisms such as phagocytosis and antibody-dependent cellular cytotoxicity.
Risk and severity of motor vehicle crashes in patients with obstructive sleep apnoea/hypopnoea
Authors: Mulgrew A T , Nasvadi G , Butt A , Cheema R , Fox N , Fleetham J A , Ryan C F , Cooper P , Ayas N T 
Published: January 2008

Background:Obstructive sleep apnoea/hypopnoea (OSAH) appears to be associated with an increased risk of motor vehicle crashes (MVCs). However, its impact on crash patterns, particularly the severity of crashes, has not been well described. A study was undertaken to determine whether OSAH severity influenced crash severity in patients referred for investigation of suspected sleep-disordered breathing.Methods:Objective crash data (including the nature of crashes) for 783 patients with suspected OSAH for the 3 years prior to polysomnography were obtained from provincial insurance records and compared with data for 783 age- and sex-matched controls. The patient group was 71% male with a mean age of 50 years, a mean apnoea-hypopnoea index (AHI) of 22 events/h and a mean Epworth Sleepiness Scale score of 10.Results:There were 375 crashes in the 3-year period, 252 in patients and 123 in controls. Compared with controls, patients with mild, moderate and severe OSAH had an increased rate of MVCs with relative risks of 2.6 (95% CI 1.7 to 3.9), 1.9 (95% CI 1.2 to 2.8) and 2.0 (95% CI 1.4 to 3.0), respectively. Patients with suspected OSAH and normal polysomnography (AHI 0–5) did not have an increased rate of MVC (relative risk 1.5 (95% CI 0.9 to 2.5), p = 0.21). When the impact of OSAH on MVC associated with personal injury was examined, patients with mild, moderate and severe OSAH had a substantially higher rate of MVCs than controls with relative risks of 4.8 (95% CI 1.8 to 12.4), 3.0 (95% CI 1.3 to 7.0) and 4.3 (95% CI 1.8 to 8.9), respectively, whereas patients without OSAH had similar crash rates to controls with a relative risk of 0.6 (95% CI 0.2 to 2.5). Very severe MVCs (head-on collisions or those involving pedestrians or cyclists) were rare, but 80% of these occurred in patients with OSAH (p = 0.06).Conclusion:Patients with OSAH have increased rates of MVCs, and disproportionately increased rates of MVCs are associated with personal injury.
DILUTED FERROMAGNETIC SEMICONDUCTORS — ORIGIN OF MAGNETIC ORDERING AND SPIN-TRANSPORT PROPERTIES
Authors: DIETL TOMASZ 
Published: January 2008

In the first hour of the lecture the present understanding of the origin of exchange interaction and mechanisms leading to ferromagnetic order in diluted magnetic semiconductors will be presented.1 The lecture will start by discussing energy positions of relevant open magnetic shells, including the correlation energy and excitations within the magnetic ions. The origin and magnitude of sp–d exchange interactions will then be described. This will be followed by presenting the physics of indirect exchange interactions between localized spins contrasting magnetic characteristics in the absence and in the presence of free carriers. The Zener and RKKY models of ferromagnetism will be introduced and the role of confinement, dimensionality, and spin-orbit interaction in determining properties of the ferromagnetic phase will be outlined. The second lecture will be devoted to theory of spin transport in layered structures of diluted ferromagnetic semiconductors, emphasizing the issues important for perspective spintronics devices. A recently developed theory,2 which combines a multi-orbital empirical tight-binding approach with a Landauer–Büttiker formalism will be presented. In contrast to the standard kp method, this theory describes properly the interfaces and inversion symmetry breaking as well as the band dispersion in the entire Brillouin zone, so that the essential for the spin-dependent transport Rashba and Dresselhaus terms as well as the tunneling via k points away from the zone center are taken into account. The applicability of this model for the description of tunneling magnetoresistance (TMR), resonant tunneling spectra, spin-current polarization in Esaki-Zener diodes, and domain-wall resistance will be presented. Note from Publisher: This article contains the abstract only.
DEVICE CONCEPTS IN SEMICONDUCTOR SPINTRONICS
Authors: MOLENKAMP LAURENS W. 
Published: January 2008

Semiconductor spintronics has now reached a stage where the basic physical mechanisms controlling spin injection and detection are understood. Moreover, some critical technological issues involved in the growth and lithography of the magnetic semiconductors have been solved. This has allowed us to explore the physics of meanwhile quite complex spintronic devices. The lectures will start with an introduction to spin transport in metals and semiconductors. Building upon this, I will discuss various simple devices that demonstrate this basic physics in action. Subsequently, more advanced devices will be covered. For example, I will discuss resonant tunneling diodes (RTDs) fabricated from paramagnetic II-VI semiconductors that can be operated as a voltage controlled spin-switch. A quantum dot version of these RTDs exhibits, unexpectedly, remanent magnetism at zero external field, which we interpret as resulting from tunneling through a single magnetic polaron. In the ferromagnetic semiconductor ( Ga, Mn ) As we have observed a very large spin valve effect due to domain wall pinning at sub-10 nm sized constrictions. Furthermore, we have found a novel magnetoresistance effect in this material, dubbed tunnel anisotropic magnetoresistance (TAMR), which is due to the strongly (magneto-)anisotropic density of states in a ferromagnetic semiconductor. The effect leads to the observation of a spin valve-like behavior in tunnel structures containg a single ferromagnetic layer and also dominates the spin-valve signal obtained from structures containing two ( Ga, Mn ) As layers, where the effect may cause resistance changes of five orders of magnitude. Note from Publisher: This article contains the abstract only.
SPIN AND PSEUDOSPIN TRANSFER
Authors: MACDONALD ALLAN 
Published: January 2008

Electrons in a Fermi liquid can be regarded as non-interacting particles (quasiparticles) in an effective potential that is a consequence of interactions between electrons. In many-body theory the effective potential is known as the electronic self-energy. When the electronic system has a broken symmetry, the effective potential can have different symmetries than the potential term that appears in the single particle Hamiltonian. For example, the effective potential of a superconductor includes terms that change the electron particle number and the effective potential of a ferromagnet includes terms that change the electron spin. When the electron system is not in equilibrium, the effective potential is altered. I will present a view of spin-transfer phenomena in ferromagnetic metals in which current-induced spin torques arise from changes in the ferromagnet's spin-dependent effective potential when the quasiparticle system is held out of equilibrium by applying a bias potential. In this view spin-transfer is an example of a more general set of phenomena and is not fundamentally associated with an approximate conservation law, for example the approximate conservation of total spin angular momentum. I will illustrate this point of view by discussing other examples of spin-transfer like phenomena, including transport anomalies that have been observed in bilayer quantum Hall systems, current-driven magnetization changes in antiferromagnetic metals, and current-drive bond-weakening in molecular electronics. Note from Publisher: This article contains the abstract only.
Sedimentary provenance and palaeoenvironment of the Baixo Araguaia Supergroup: constraints on the palaeogeographical evolution of the Araguaia Belt and assembly of West Gondwana
Authors: Moura C. A. V. , Pinheiro B. L. S. , Nogueira A. C. R. , Gorayeb P. S. S. , Galarza M. A. 
Published: January 2008

Abstract

Provenance studies on metasedimentary rocks of the Baixo Araguaia Supergroup of the Brasiliano Araguaia Belt, central Brazil, yield
207
Pb/
206
Pb zircon evaporation ages for detrital zircons from quartzites concentrated around 1000–1200 Ma and 2800–2900 Ma; Sm–Nd
T
DM
model ages of schists and phyllites scatter around 1600–1700 Ma. Facies analysis of low-grade metasedimentary rocks from drill cores suggests a sedimentary environment of basin floor and lower- to upper-slope turbidites. Nearby sources are indicated by the textural and mineralogical immaturity; together with structural geological data indicating tectonic transport of the supracrustal pile towards the NW, this suggests probable provenance from the southeastern portion of the Araguaia Belt and not from the Amazonian Craton as usually believed. The Goiás Massif, Goiás Magmatic Arc, São Francisco Craton and Paranapanema block are considered to be the best candidates. They may have formed a larger continental mass during West Gondwana amalgamation, prior to their collision with the Amazonian Craton to form the Araguaia Belt. Final timing of this collision is constrained by
c
. 550 Ma syntectonic granites. Similar ages for high-grade gneisses in the Rokelide Belt suggest coeval collision and coetaneous metamorphism of the Araguaia and Rokelide belts, but more geological and geophysical data are required for a decisive correlation between these belts.
Tectonic evolution of the Brasília Belt, Central Brazil, and early assembly of Gondwana
Authors: Valeriano C. M. , Pimentel M. M. , Heilbron M. , Almeida J. C. H. , Trouw R. A. J. 
Published: January 2008

Abstract

The Brasília Belt comprises terranes and thrust-sheets that were tectonically transported towards the western passive margin of the São Francisco–Congo palaeocontinent during an orogenic episode resulting from collision of the Paranapanema and Goiás blocks and the Goiás magmatic arc against São Francisco–Congo at 0.64–0.61 Ga. The tectonic zones of the belt are, from east to west: a foreland zone with Archaean–Palaeoproterozoic granite–greenstone basement covered by Neoproterozoic anchimetamorphic sedimentary rocks (Bambuí Group); a low metamorphic grade thrust-fold belt of proximal shelf successions, mostly siliciclastic, containing rare basement slivers; metamorphic nappes in upper greenschist to granulite facies of distal shelf and slope metasediments and subordinate tholeiitic metabasalts; the Goiás massif, possibly a microcontinent; and the Goiás magmatic arc. The accretion of these terranes against the western margin of the São Francisco–Congo palaeocontinent took place during an early phase of Gondwana supercontinent amalgamation, when terranes accreted around São Francisco–Congo to create a proto-West Gondwana landmass, around which subsequent collisional and accretionary events followed, such as those in the Borborema–Trans-Saharan province (
c.
0.62–0.60 Ga); in the Ribeira–Araçuaí belt (
c.
0.58 Ga); along the Araguaia and Paraguay belts (collision of Amazonia,
c.
0.54–0.52 Ga); and the accretion of Cabo Frio terrane in the Ribeira Belt (
c.
0.53–0.50 Ga).
Correlation of Neoproterozoic terranes between the Ribeira Belt, SE Brazil and its African counterpart: comparative tectonic evolution and open questions
Authors: Heilbron M. , Valeriano C. M. , Tassinari C. C. G. , Almeida J. , Tupinambá M. , Siga O. , Trouw R. 
Published: January 2008

Abstract

Four main classes of tectonic entities may be considered for the Ribeira Belt and southwest African counterparts: (1) cratonic fragments older than 1.8 Ga and their passive margin successions, (2) reworked basement terranes with Mesoproterozoic and/or Neoproterozoic deformed cover, (3) magmatic arc associations, (4) terranes with Palaeoproterozoic basement and deformed Neoproterozoic back-arc successions. Based on comparative investigation, a tectonic model of polyphase amalgamation is proposed with
c
. 790 and 630–610 Ma major episodes of intra-oceanic and cordilleran arc magmatism along both sides of the Adamastor Ocean. Subsequent diachronous collision of the arc terranes and small plates followed at
c
. 630, 600, 580 and 530 Ma. The tectonic complexity reflects an accretionary evolution from Cryogenian to Cambrian times. The São Francisco–Congo and Angola palaeo-continents did probably not behave as one consolidated block, but rather may have accommodated considerable convergence during the Brasiliano/Pan-African episodes. The final docking of Cabo Frio and Kalahari in the Cambrian was coeval with the arrival of Amazonia on the opposite side, resulting in lateral reactivation and displacement between the previously amalgamated pieces. The transition between the Cambrian and the Ordovician is marked by the extensional collapse of the metamorphic core zones of the orogens.
West Gondwana amalgamation based on detrital zircon ages from Neoproterozoic Ribeira and Dom Feliciano belts of South America and comparison with coeval sequences from SW Africa
Authors: Basei M. A. S. , Frimmel H. E. , Nutman A. P. , Preciozzi F. 
Published: January 2008

Abstract
Neoproterozoic–Cambrian amalgamation of West Gondwana involved the collision of several terranes of older crust that are now in eastern South America and western Africa. U–Pb (SHRIMP) detrital zircon ages from representative metasedimentary units of the Ribeira and Dom Feliciano belts (South America) and Gariep and Damara belts (Africa) provide constraints on the possible sediment source areas across probable suture zones. Ribeira detrital zircons are Palaeoproterozoic and Archaean. For the Dom Feliciano Belt, a contribution of Meso- and Neoproterozoic zircons is present, which definitely indicate Neoproterozoic sedimentation. It is proposed that the inflow of material to the Ribeira basin was essentially derived from the Paranapanema and Rio de la Plata cratons, whereas for the Damara and Gariep–Rocha belts source areas were from the Namaqua Belt. The Dom Feliciano Belt received sediments from the South American side and to a lesser degree from African sources. These results highlight the differences in the detrital zircon signatures across a proposed West Gondwanan suture, with those in the west being derived from distinctive South American basement sources and those in the east from distinctive African sources.
A Damara orogen perspective on the assembly of southwestern Gondwana
Authors: Gray D. R. , Foster D. A. , Meert J. G. , Goscombe B. D. , Armstrong R. , Trouw R. A. J. , Passchier C. W. 
Published: January 2008

Abstract

The Pan-African Damara orogenic system records Gondwana amalgamation involving serial suturing of the Congo–São Francisco and Río de la Plata cratons (North Gondwana) from 580 to 550 Ma, before amalgamation with the Kalahari–Antarctic cratons (South Gondwana) as part of the 530 Ma Kuunga–Damara orogeny. Closure of the Adamastor Ocean was diachronous from the Araçuaí Belt southwards, with peak sinistral transpressional deformation followed by craton overthrusting and foreland basin development at 580–550 Ma in the Kaoko Belt and at 545–530 Ma in the Gariep Belt. Peak deformation/metamorphism in the Damara Belt was at 530–500 Ma, with thrusting onto the Kalahari Craton from 495 Ma through to 480 Ma. Coupling of the Congo and Río de la Plata cratons occurred before final closure of the Mozambique and Khomas (Damara Belt) oceans with the consequence that the Kuunga suture extends into Africa as the Damara Belt, and the Lufilian Arc and Zambezi Belt of Zambia. Palaeomagnetic data indicate that the Gondwana cratonic components were in close proximity by
c.
550 Ma, so the last stages of the Damara–Kuunga orogeny were intracratonic, and led to eventual out-stepping of deformation/metamorphism to the Ross–Delamerian orogen (
c.
520–500 Ma) along the leading edge of the Gondwana supercontinental margin.
The Neoproterozoic glacial record in the Río de la Plata Craton: a critical reappraisal
Authors: Pazos P. J. , Bettucci L. S. , Loureiro J. 
Published: January 2008

Abstract
Neoproterozoic glacial successions have been described in South America, but the glacial deposits of the Río de la Plata Craton have been neglected in previous studies addressing the global distribution of glacially influenced successions. The Río de la Plata Craton contains Neoproterozoic glacial deposits in the Sierra del Volcán Formation (Tandilia System, Argentina), glacial influenced deposits in the Playa Hermosa and Zanja del Tigre formations (Dom Feliciano Belt, Uruguay) and suspected glacially influenced deposits in Passo da Areia (São Gabriel block, Brazil). The Tandilia System glacial record includes diamictites, dropstones and rhythmites deposited in glaciomarine conditions in a tectonically stable depositional setting. The Dom Feliciano Belt includes a thin section with ice-rafted clasts in carbonates and a thicker section containing diamictites, rhythmites, outsized clasts and deformed beds in a volcano-sedimentary succession. The São Gabriel block occurrence deserves more attention to confirm any glacial influence in the fine-grained part of the succession. Glaciation is considered to be contemporaneous with the Gaskiers glaciation (580 Ma), with the exception of the carbonates with dropstones that may represent a previous event correlative with one of the glaciations described in the Kalahari Craton, prior to Kalahari–Río de la Plata assembly in the proto-western Gondwana margin.