bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved investment in mental health services: value for money?
Authors: Goldberg David 
Published: February 2008

BackgroundThis paper examines what has been achieved in the specialist mental health services by the vastly increased health expenditures that the National Health Service (NHS) has enjoyed in the past 5 years.AimsTo describe the way money has been spent in specialist mental health services and examine why problems remain after such admirable changes to already available resources.MethodChanges in staff employed by mental health services, where the extra staff are deployed, and patterns of expenditure within the whole service and within community mental health teams are examined.ResultsSome of the new expenditure has been well spent, and has produced improvements in the service. However, one must also take account of the costs of the greatly increased numbers of managers, who impose two sorts of costs: that of their own salaries, and the opportunity costs of front-line staff having to attend meetings and write reports rather than seeing patients. Throughout the rest of the NHS, money has been wasted on needless reorganisations, on consultant and general practitioner contracts, and on information technology that has so far failed to deliver tangible advantages.ConclusionsThe emphasis on central control undermines local initiatives and wastes resources. Some central control is inevitable, but policies need to be developed in collaboration with clinicians. At local level, expenditure by primary care trusts and mental health trusts also needs to be scrutinised by committees that should include representatives of front-line mental health staff.
Metabotropic glutamate receptor agonists for schizophrenia
Authors: Harrison Paul J. 
Published: February 2008

SummaryA drug acting at metabotropic glutamate receptors has recently been reported to be an effective antipsychotic, breaking the rule that only dopamine receptor-blocking drugs have this property. The finding complements accumulating evidence that glutamatergic abnormalities are important in the pathophysiology of schizophrenia.
Free-flight encounters between praying mantids (Parasphendale agrionina) and bats (Eptesicus fuscus)
Authors: Triblehorn J. D. , Ghose K. , Bohn K. , Moss C. F. , Yager D. D. 
Published: February 2008

SUMMARYThrough staged free-flight encounters between echolocating bats and praying mantids, we examined the effectiveness of two potential predator-evasion behaviors mediated by different sensory modalities: (1) power dive responses triggered by bat echolocation detected by the mantis ultrasound-sensitive auditory system, and (2) `last-ditch' maneuvers triggered by bat-generated wind detected by the mantis cercal system. Hearing mantids escaped more often than deafened mantids (76% vs 34%, respectively; hearing conveyed 42%advantage). Hearing mantis escape rates decreased when bat attack sequences contained very rapid increases in pulse repetition rates (escape rates &lt;40%for transition slopes &gt;16 p.p.s. 10 ms–1; escape rates&gt;60% for transition slopes &lt;16 p.p.s. 10 ms–1). This suggests that echolocation attack sequences containing very rapid transitions(&gt;16 p.p.s. 10 ms–1) could circumvent mantis/insect auditory defenses. However, echolocation attack sequences containing such transitions occurred in only 15% of the trials. Since mantis ultrasound-mediated responses are not 100% effective, cercal-mediated evasive behaviors triggered by bat-generated wind could be beneficial as a backup/secondary system. Although deafened mantids with functioning cerci did not escape more often than deafened mantids with deactivated cerci (35%vs 32%, respectively), bats dropped mantids with functioning cerci twice as frequently as mantids with deactivated cerci. This latter result was not statistically reliable due to small sample sizes, since this study was not designed to fully evaluate this result. It is an interesting observation that warrants further investigation, however, especially since these dropped mantids always survived the encounter.
Swelling-activated chloride channels in leech Retzius neurons
Authors: Coulon Philippe , Wüsten Hans-Joachim , Hochstrate Peter , Dierkes Paul Wilhelm 
Published: February 2008

SUMMARYDuring periods of high activity neurons are expected to swell due to the uptake of Cl–. To find out whether leech Retzius neurons possess swelling-activated Cl– channels that facilitate Cl– efflux and, hence, volume recovery, we exposed the cells to hypotonic solutions. In hypotonic solutions, the cells slowly swelled but did not undergo a regulatory volume decrease. However, the cell volume increased less than predicted for an ideal osmometer, suggesting the action of a compensatory mechanism. The cell swelling was paralleled by a marked decrease in the input resistance as well as by the activation of a membrane current with a reversal potential close to the Cl–equilibrium potential. This current was substantially diminished by removing bath Cl–, by applying the Cl– channel blocker DIDS, or by treating the cells with the tubulin polymerization inhibitor colchicine. Furthermore, in the presence of colchicine or vinblastine, the cell swelling was substantially increased. It is concluded that leech Retzius neurons possess swelling-activated Cl–channels that require an intact microtubule system for activation. The channels may help to restore cell volume after periods of high neuronal activity.
Sodium uptake in different life stages of crustaceans: the water fleaDaphnia magnaStrauss
Authors: Bianchini Adalto , Wood Chris M. 
Published: February 2008

SUMMARYThe concentration-dependent kinetics and main mechanisms of whole-body Na+ uptake were assessed in neonate and adult water flea Daphnia magna Strauss acclimated to moderately hard water (0.6 mmol l–1 NaCl, 1.0 mmol l–1 CaCO3 and 0.15 mmol l–1 MgSO4·7H2O; pH 8.2). Whole-body Na+ uptake is independent of the presence of Cl– in the external medium and kinetic parameters are dependent on the life stage. Adults have a lower maximum capacity of Na+ transport on a mass-specific basis but a higher affinity for Na+ when compared to neonates. Based on pharmacological analyses,mechanisms involved in whole-body Na+ uptake differ according to the life stage considered. In neonates, a proton pump-coupled Na+channel appears to play an important role in the whole-body Na+uptake at the apical membrane. However, they do not appear to contribute to whole-body Na+ uptake in adults, where only the Na+channel seems to be present, associated with the Na+/H+exchanger. In both cases, carbonic anhydrase contributes by providing H+ for the transporters. At the basolateral membrane of the salt-transporting epithelia of neonates, Na+ is pumped from the cells to the extracellular fluid by a Na+,K+-ATPase and a Na+/Cl– exchanger whereas K+ and Cl– move through specific channels. In adults, a Na+/K+/2Cl– cotransporter replaces the Na+/Cl– exchanger. Differential sensitivity of neonates and adults to iono- and osmoregulatory toxicants, such as metals, are discussed with respect to differences in whole-body Na+ uptake kinetics, as well as in the mechanisms of Na+ transport involved in the whole-body Na+ uptake in the two life stages.
Cuticular hydrocarbons as maternal provisions in embryos and nymphs of the cockroach Blattella germanica
Authors: Fan Yongliang , Eliyahu Dorit , Schal Coby 
Published: February 2008

SUMMARY
Cuticular hydrocarbons of arthropods serve multiple functions, including as barriers to water loss and as pheromones and pheromone precursors. In the oviparous German cockroach, Blattella germanica, long-chain hydrocarbons are produced by oenocytes within the abdominal integument and are transported by a blood lipoprotein, lipophorin, both to the cuticular surface and into vitellogenic oocytes. Using radiotracer approaches, we tracked the location and metabolic fate of 14C- and 3H-labeled hydrocarbons through vitellogenic females and their embryos and nymphs. A considerable amount (∼50%) of radiolabeled maternal hydrocarbons was transferred to oocytes and persisted through a 20-day embryogenesis and the first two nymphal stadia. The maternal hydrocarbons were not degraded or lost during this protracted period, except for significant losses of cuticular hydrocarbons starting with the first-to-second instar molt. Thus, although embryos and nymphs can produce their own hydrocarbons, maternal hydrocarbons provide a significant fraction of the cuticular and hemolymph hydrocarbons of both stages. These results show, for the first time in any insect, that a mother provides a significant complement of her offspring's cuticular hydrocarbons. Further research will be needed to determine whether provisioning hydrocarbons to eggs is a general strategy among insects and other arthropods or if this strategy is limited to taxa where eggs and early instars are susceptible to desiccation.
The hygric hypothesis does not hold water: abolition of discontinuous gas exchange cycles does not affect water loss in the antCamponotus vicinus
Authors: Lighton John R. B. , Turner Robbin J. 
Published: February 2008

SUMMARYThe discontinuous gas exchange cycle (DGC) of insects and other tracheate arthropods temporally decouples oxygen uptake and carbon dioxide emission and generates powerful concentration gradients for both gas species between the outside world and the tracheal system. Although the DGC is considered an adaptation to reduce respiratory water loss (RWL) – the `hygric hypothesis' – it is absent from many taxa, including xeric ones. The`chthonic hypothesis' states that the DGC originated as an adaptation to gas exchange in hypoxic and hypercapnic, i.e. underground, environments. If that is the case then the DGC is not the ancestral condition, and its expression is not necessarily a requirement for reducing RWL. Here we report a study of water loss rate in the ant Camponotus vicinus, measured while its DGC was slowly eliminated by gradual hypoxia (hypoxic ramp de-DGCing). Metabolic rate remained constant. The DGC ceased at a mean PO2 of 8.4 kPa. RWL in the absence of DGCs was not affected until PO2 declined below 3.9 kPa. Below that value, non-DGC spiracular regulation failed, accompanied by a large increase in RWL. Thus, the spiracular control strategy of the DGC is not required for low RWL, even in animals that normally express the DGC.
Characterisation of neurotransmitter-induced electrolyte transport in cockroach salivary glands by intracellular Ca2+, Na+ and pH measurements in duct cells
Authors: Hille Carsten , Walz Bernd 
Published: February 2008

SUMMARYIon-transporting acinar peripheral cells in cockroach salivary glands are innervated by dopaminergic and serotonergic fibres, but saliva-modifying duct cells are innervated only by dopaminergic fibres. We used microfluorometry to record intracellular Na+, Ca2+ and H+concentrations ([Na+]i, [Ca2+]iand pHi) in duct cells of two types of preparation, viz`lobes' consisting of acini with their duct system and `isolated ducts'without acini, in order to obtain information about the transporters involved in saliva secretion and/or modification. Our results indicate that (1)stimulation of lobes by dopamine (DA) causes a strong drop of pHiand increases in [Na+]i and[Ca2+]i in duct cells; (2) in contrast, DA stimulation of isolated ducts produces only a small pHi drop and no changes in[Na+]i and [Ca2+]i; (3)pHi and [Ca2+]i changes are also induced in duct cells by serotonin (5-HT) stimulation of lobes, but not isolated ducts;(4) in the absence of CO2/HCO3–, the DA-induced pHi drop is strongly reduced by removal of extracellular Cl– or inhibition of the Na+–K+–2Cl– cotransporter(NKCC); (5) in the presence of CO2/HCO3–, the DA-induced pHi drop is not reduced by NKCC inhibition, but rather by inhibition of the Cl–/HCO3–exchanger (AE), Na+/H+ exchanger (NHE) or carbonic anhydrase. We suggest that DA and 5-HT act predominantly on acinar peripheral cells. Their activity (secretion of primary saliva) seems to cause changes in ion concentrations in duct cells. NKCC and/or AE/NHE activities are necessary for pHi changes in duct cells; we consider that these transporters are involved in the secretion of the NaCl-rich primary saliva.
Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin
Authors: Ivanina Anna V. , Cherkasov Anton S. , Sokolova Inna M. 
Published: February 2008

SUMMARY
Cadmium (Cd) is an important toxicant in estuarine and coastal environments that can strongly affect energy balance of aquatic organisms by increasing the organism's basal energy demand and reducing its aerobic capacity. Mechanisms of cadmium-induced increase in basal metabolic costs are not well understood and may involve elevated detoxification costs due to the synthesis of cellular protective proteins and glutathione. We studied the short-term effects of cadmium exposure (4 h) on protein and glutathione (GSH) synthesis and expression of stress proteins (heat shock proteins HSP60, HSP70 and HSP90) and metallothioneins in isolated gill and hepatopancreas cells of the eastern oyster, Crassostrea virginica. Our study showed that exposure to cadmium resulted in a dose-dependent increase in the rate of protein synthesis in oyster cells, which reached 150% of the control at the highest tested Cd level (2000 μmol l–1). GSH synthesis was significantly inhibited by the highest Cd concentrations, especially in hepatopancreas,which resulted in a slight but significant decrease in the total GSH concentrations. Elevated protein synthesis was associated with the increased expression of metallothioneins and heat shock proteins. Interestingly, stress protein response differed considerably between gill and hepatopancreas cells. In hepatopancreas, expression of metallothionein mRNA (measured by real-time PCR) increased 2–8-fold in response to Cd exposure, whereas no significant increase in metallothionein expression was found in Cd-exposed gill cells. By contrast, HSP60 and HSP70 protein levels increased significantly in Cd-exposed gill cells (by 1.5–2-fold) but not in hepatopancreas. No change in HSP90 expression was detected in response to Cd exposure in oyster cells. These data indicate that metallothionein expression may provide sufficient protection against Cd-induced damage to intracellular proteins in hepatopancreas, alleviating the need for overexpression of molecular chaperones. By contrast, Cd detoxification mechanisms such as inducible metallothioneins and GSH appear to be insufficient to fully prevent protein damage in gill cells, thus necessitating induction of HSPs as a secondary line of cellular defense. Therefore, gills are likely to be among the most Cd-sensitive tissues in oysters, which may have important implications for impaired oxygen uptake contributing to energy misbalance and reduced aerobic scope in Cd-exposed oysters.
Cryoprotective dehydration and the resistance to inoculative freezing in the Antarctic midge, Belgica antarctica
Authors: Elnitsky Michael A. , Hayward Scott A. L. , Rinehart Joseph P. , Denlinger David L. , Lee Richard E. 
Published: February 2008

SUMMARY
During winter, larvae of the Antarctic midge, Belgica antarctica(Diptera, Chironomidae), must endure 7–8 months of continuous subzero temperatures, encasement in a matrix of soil and ice, and severely desiccating conditions. This environment, along with the fact that larvae possess a high rate of water loss and are extremely tolerant of desiccation, may promote the use of cryoprotective dehydration as a strategy for winter survival. This study investigates the capacity of larvae to resist inoculative freezing and undergo cryoprotective dehydration at subzero temperatures. Slow cooling to–3°C in an environment at equilibrium with the vapor pressure of ice reduced larval water content by ∼40% and depressed the body fluid melting point more than threefold to –2.6°C. This melting point depression was the result of the concentration of existing solutes (i.e. loss of body water) and the de novo synthesis of osmolytes. By day 14 of the subzero exposure, larval survival was still &gt;95%, suggesting larvae have the capacity to undergo cryoprotective dehydration. However, under natural conditions the use of cryoprotective dehydration may be constrained by inoculative freezing as result of the insect's intimate contact with environmental ice. During slow cooling within a substrate of frozen soil, the ability of larvae to resist inoculative freezing and undergo cryoprotective dehydration was dependent upon the moisture content of the soil. As detected by a reduction of larval water content, the percentage of larvae that resisted inoculative freezing increased with decreasing soil moisture. These results suggest that larvae of the Antarctic midge have the capacity to resist inoculative freezing at relatively low soil moisture contents and likely undergo cryoprotective dehydration when exposed to subzero temperatures during the polar winter.
RGD-dependent mechanisms in the endoneurial phagocyte response and axonal regeneration in the nervous system of the snail Lymnaea stagnalis
Authors: Hermann Petra M. , Nicol Jennifer J. , Bulloch Andrew G. M. , Wildering Willem C. 
Published: February 2008

SUMMARY
Activation of phagocytic cells in the injury zone is a crucial step in the regeneration of peripheral axons. Many aspects of the mechanisms underlying the recruitment of active phagocytes remain, however, unclear. Notably, our understanding of the interactions between injury, extracellular matrix (ECM)degradation and phagocyte activation is limited. Most animal cell types,phagocytes included, interact with proteins of the ECM through one or more members of the integrin family, transmembrane cell adhesion receptors that typically bind their ligands through short linear amino acid sequences. This study focused on the role of one of the most common of such integrin recognition sequences, the Arg-Gly-Asp (RGD) motif in the recruitment and activation of endoneurial phagocytes in the injury response of the nervous system of the pond snail Lymnaea stagnalis. Like the mammalian nervous system, the Lymnaea nervous system responds to injury with recruitment and activation of endoneurial phagocytes (i.e. phagocytes residing in Lymnaea's nerves), a process involving substantial changes in the morphology, motility and adhesion status of these cells. Using synthetic water-soluble RGD-peptides, we investigated the relevance of RGD-dependent mechanisms in the activation of endoneurial phagocytes and injury response of the organ-cultured nervous system of Lymnaea. Our results show that RGD-peptides modulate various aspects of phagocyte activation (i.e. spreading response, particle engulfment, oxidative burst) in vitro and in situ and significantly affect nerve regeneration in this model system. Surprisingly, while linear RGD-analogues suppressed both phagocyte activation and axonal regeneration, a circularized RGD-peptide analogue modulated these parameters in a concentration-dependent, biphasic manner. Collectively, these results emphasize the significance of RGD-dependent mechanisms in the regenerative response of the Lymnaea nervous system and implicate regulation of the cellular immune response as one of the factors in this context.
Competition between immune function and lipid transport for the protein apolipophorin III leads to stress-induced immunosuppression in crickets
Authors: Adamo S. A. , Roberts J. L. , Easy R. H. , Ross N. W. 
Published: February 2008

SUMMARY
Intense physical activity results in transient immunosuppression in a wide range of animals. We tested the hypothesis that competition between immune function and lipid transport for the protein apolipophorin III (apoLpIII) can cause transient immunosuppression in crickets. Both flying, an energetically demanding behavior, and an immune challenge reduced the amount of monomeric(free) apoLpIII in the hemolymph of crickets. Because both immune function and flying depleted free apoLpIII, these two phenomena could be in competition for this protein. We showed that immune function was sensitive to the amount of free apoLpIII in the hemolymph. Reducing the amount of free apoLpIII in the hemolymph using adipokinetic hormone produced immunosuppression. Increasing apoLpIII levels after flight by pre-loading animals with trehalose reduced immunosuppression. Increasing post-flight apoLpIII levels by injecting purified apoLpIII also reduced flight-induced immunosuppression. These results show that competition between lipid transport and immune function for the same protein can produce transient immunosuppression after flight-or-fight behavior. Intertwined physiological systems can produce unexpected trade-offs.
Shipboard measurements of the hearing of the white-beaked dolphin Lagenorhynchus albirostris
Authors: Nachtigall P. E. , Mooney T. A. , Taylor K. A. , Miller L. A. , Rasmussen M. H. , Akamatsu T. , Teilmann J. , Linnenschmidt M. , Vikingsson G. A. 
Published: February 2008

SUMMARY
This is the first report of an underwater audiogram from a dolphin in a capture-and-release scenario. Two bow-riding white-beaked dolphins Lagenorhynchus albirostris (a female and a male) were captured using the hoop-net technique in Faxaflói Bay, Iceland. The dolphins were transferred to a stretcher and hoisted into a plastic research tank on board a small fishing vessel. Two underwater transducers were used to cover the frequency range from 16 to 215 kHz. Two human EEG electrodes mounted in suction cups, one placed near the blow hole and the other on the dorsal fin,picked up bioelectrical responses to acoustic stimuli. Responses to about 1000 sinusoidal amplitude modulated stimuli for each amplitude/frequency combination were averaged and analyzed using a fast Fourier transform to obtain an evoked auditory response. Threshold was defined as the zero crossing of the response using linear regression. Two threshold frequencies at 50 kHz and 64 kHz were obtained from the female. An audiogram ranging from 16 to 181 kHz was obtained from an adult male and showed the typical `U' shaped curve for odontocetes. The thresholds for both white-beaks were comparable and demonstrated the most sensitive high frequency hearing of any known dolphin and were as sensitive as the harbor porpoise.
Morphological and biochemical changes in the Harderian gland of hypothyroid rats
Authors: Monteforte Rossella , Santillo Alessandra , Lanni Antonia , D'Aniello Salvatore , Baccari Gabriella Chieffi 
Published: February 2008

SUMMARY
The secretory activity of the Harderian gland (HG) is influenced by both exogenous (such as light and temperature) and endogenous (such as prolactin,thyroid hormones and steroid hormones) factors, which vary among species. In the present study, the effects of hypothyroidism on the rat HG were examined at morphological and biochemical levels. The decrease in cytoplasmic lipoproteic vacuoles and the increase in mucosubstance secretion in the acinar lumina were the most notable histological effects elicited by hypothyroidism. The release of all granules with nuclei and cellular debris suggested the occurrence of holocrine secretion. Electron microscopy revealed in the glandular cells of hypothyroid rat an increased condensation of chromatin in the nuclei, mitochondria with decreased cristae and vacuolisation, decreased glycogen granules, autophagic vacuoles, and lipofuscins in the cytoplasm. TUNEL reaction indicated DNA fragmentation in hypothyroid HG, indicative of an underlying apoptotic process. Translocation of cytochrome c from mitochondria to cytosol strongly supported this hypothesis. In conclusion, these findings indicate that thyroid hormones play a pivotal role in preserving the structural integrity of the rat HG and, hence, its secretory activity.