bluuebunny/crossref_metadata_2025_split huggingface dataset:
Operative Treatment of Ulnar Collateral Ligament Insufficiency of the Elbow in Female Athletes
Authors: Argo David , Trenhaile Scott W. , Savoie Felix H. , Field Larry D. 
Published: November 2005

Background As women become more involved in athletic activity, injuries specific to this population also increase. No data exist regarding operative treatment of female patients with elbow instability secondary to ulnar collateral ligament insufficiency. Hypothesis Women with symptomatically unstable elbows who failed nonoperative treatment will improve after surgical intervention. Study Design Case series; Level of evidence, 4. Methods Nineteen women were retrospectively evaluated using the Andrews and Carson Elbow Outcome Score. Patients were included if they underwent repair or reconstruction of the ulnar collateral ligament for symptomatic instability that precluded them from participation in their desired sport or activities, despite nonoperative treatment. Results The mean age was 22.0 years (range, 15.1-37.2 years). The mean follow-up was 38.8 months (range, 12.4-68.6 months). Of the 19 patients, 14 were softball players, gymnasts, and tennis players. Only 1 was a pitcher. Eighteen women underwent repair by one of the following procedures: plication (n = 6), repair to bone using anchors (n = 11), or drill holes (n = 1). One patient underwent a palmaris graft reconstruction. The mean overall preoperative outcome score of 120 improved to 191 postoperatively (P< .0001). Seventeen of 18 athletes were able to return to their sport at a mean of 2.5 months postoperatively. One patient did not participate in athletics. Conclusion This study demonstrated excellent overall results in 16 and good results in 3 female patients after medial elbow repair or reconstruction. Women appear to be able to consistently return to a high level of function after repair or reconstruction for medial elbow instability.
The Effects of Plyometric versus Dynamic Stabilization and Balance Training on Lower Extremity Biomechanics
Authors: Myer Gregory D. , Ford Kevin R. , McLean Scott G. , Hewett Timothy E. 
Published: November 2005

Background Neuromuscular training that includes both plyometric and dynamic stabilization/balance exercises alters movement biomechanics and reduces ACL injury risk in female athletes. The biomechanical effects of plyometric and balance training utilized separately are unknown. Hypothesis A protocol that includes balance training without plyometric training will decrease coronal plane hip, knee, and ankle motions during landing, and plyometric training will not affect coronal plane measures. The corollary hypothesis was that plyometric and balance training effects on knee flexion are dependent on the movement task tested. Study Design Controlled laboratory study. Methods Eighteen high school female athletes participated in 18 training sessions during a 7-week period. The plyometric group (n= 8) performed maximum-effort jumping and cutting exercises, and the balance group (n = 10) used dynamic stabilization/ balance exercises during training. Lower extremity kinematics were measured during the drop vertical jump and the medial drop landing before and after training using 3D motion analysis techniques. Results During the drop vertical jump, both plyometric and balance training reduced initial contact (P= .002), maximum hip adduction angle (P= .015), and maximum ankle eversion angle (P= .020). During the medial drop landing, both groups decreased initial contact (P= .002) and maximum knee abduction angle (P= .038). Plyometric training increased initial contact knee flexion (P= .047) and maximum knee flexion (P= .031) during the drop vertical jump, whereas the balance training increased maximum knee flexion (P= .005) during the medial drop landing. Conclusion Both plyometric and balance training can reduce lower extremity valgus measures. Plyometric training affects sagittal plane kinematics primarily during a drop vertical jump, whereas balance training affects sagittal plane kinematics during single-legged drop landing. Clinical Relevance Both plyometric and dynamic stabilization/balance exercises should be included in injury-prevention protocols.
Kinematics and Kinetics of Elite Windmill Softball Pitching
Authors: Werner Sherry L. , Jones Deryk G. , Guido John A. , Brunet Michael E. 
Published: November 2005

Background A significant number of time-loss injuries to the upper extremity in elite windmill softball pitchers has been documented. The number of outings and pitches thrown in 1 week for a softball pitcher is typically far in excess of those seen in baseball pitchers. Shoulder stress in professional baseball pitching has been reported to be high and has been linked to pitching injuries. Shoulder distraction has not been studied in an elite softball pitching population. Hypothesis The stresses on the throwing shoulder of elite windmill pitchers are similar to those found for professional baseball pitchers. Study Design Descriptive laboratory study. Methods Three-dimensional, high-speed (120 Hz) video data were collected on rise balls from 24 elite softball pitchers during the 1996 Olympic Games. Kinematic parameters related to pitching mechanics and resultant kinetics on the throwing shoulder were calculated. Multiple linear regression analysis was used to relate shoulder stress and pitching mechanics. Results Shoulder distraction stress averaged 80% of body weight for the Olympic pitchers. Sixty-nine percent of the variability in shoulder distraction can be explained by a combination of 7 parameters related to pitching mechanics. Conclusion Excessive distraction stress at the throwing shoulder is similar to that found in baseball pitchers, which suggests that windmill softball pitchers are at risk for overuse injuries. Normative information regarding upper extremity kinematics and kinetics for elite softball pitchers has been established.
Effects of Knee Flexion Angles for Graft Fixation on Force Distribution in Double-Bundle Anterior Cruciate Ligament Grafts
Authors: Miura Kazutomo , Woo Savio L.-Y. , Brinkley Ragan , Fu Yin-Chih , Noorani Sabrina 
Published: November 2005

Background In double-bundle anterior cruciate ligament reconstruction, overloading either 1 of the 2 grafts should be avoided to decrease the risk of graft failure. Hypothesis Overloading of the posterolateral graft may occur when it is fixed at 30 ° of knee flexion because the posterolateral bundle is elongated as the knee approaches extension. Study Design Controlled laboratory study. Methods Ten human cadaveric knees were tested at (1) intact, (2) anterior cruciate ligament–deficient, (3) double-bundle anterior cruciate ligament reconstruction with the anteromedial and posterolateral grafts fixed at 60 ° of flexion and full extension, respectively (fixation 60/FE), and (4) double-bundle anterior cruciate ligament reconstruction with both grafts fixed at 30 ° of flexion simultaneously (fixation 30/30). Two external loading conditions simulating clinical examinations were used: (1) 134-N anterior tibial load and (2) combined rotatory loads of 10 N·m valgus and 5 N·m internal tibial torques. Data on knee kinematics and in situ forces in the 2 bundles of the intact anterior cruciate ligament and the respective grafts were obtained. Results In response to 134-N anterior tibial load, knee kinematics and in situ force in the grafts were similar to the intact knee for both fixation protocols. The force in the anteromedial graft for fixation 60/FE was 34% higher, whereas the posterolateral graft for fixation 30/30 was 46% higher, compared with the intact anteromedial and posterolateral bundles, respectively. In response to combined rotatory loads, the posterolateral graft for fixation 30/30 carried 67% higher load than did the intact posterolateral bundle. Conclusion Fixation 30/30 overloaded the posterolateral graft, whereas fixation 60/FE overloaded the anteromedial graft. Clinical Relevance In double-bundle anterior cruciate ligament reconstruction, even though overall forces in the graft are the same as intact anterior cruciate ligament, the force distributions may not be the same as the intact bundles, and overloading of 1 of the 2 grafts may occur.
An American Reflects on the Windsor Report
Authors: Douglas Ian T. 
Published: November 2005

ABSTRACTThe Windsor Report of the Lambeth Commission on Communion was published in late 2004 and has been released with encouragement from the Archbishop of Canterbury that it be studied around the Anglican Communion. A ‘reception’ process has been established to solicit input and monitor discussion on the Windsor Report from across the Anglican Communion. This article is a reflection on the report from the perspective of an American Episcopalian missiologist and was offered as part of the ‘reception’ process. In a step-by-step examination of the contents of the Windsor Report, the reflection presents underlying currents, concerns and possibilities for the Anglican Communion arising from the Report. There is much of benefit in the Report while at the same time dissent is registered with some of its presuppositions and findings.
Doubts about Doubt: Honest to God Forty Years On
Authors: Wright N. T. 
Published: November 2005

ABSTRACTHonest to God, published in 1963, was one of the most public religious bestsellers of the twentieth century. Because it was written by an Anglican bishop it was especially controversial. Yet there are questions that remain and this article highlights seven such questions which draw attention to weaknesses in the book. An alternative proposal is offered here. Robinson had his finger on a real problem in postwar British church life and, in a measure, theology. I believe the problem was mostly or largely caused not by the New Testament and historic Christianity itself, but by the way in which the post-Enlightenment world had assimilated and re-expressed the Christian faith. What Robinson referred to when speaking of supraor supernaturalism belonged within an essentially Deist or Epicurean framework, and he was struggling with the unwelcome consequences of people being unable to relate to their absentee landlord, and simultaneously puzzling over the fact that some people did not find this a problem. The huge popularity of his book shows that he struck a chord with a great many people. The tragedy of Honest to God, as I perceive it, is that Robinson did not see that what he was rejecting was a form of supernaturalism pressed upon Christianity by the Enlightenment; that he did not therefore go looking for help in finding other ways of holding together what the classic Christian tradition has claimed about God.
Anglican Approaches to Christian–Muslim Dialogue
Authors: Ipgrave Michael 
Published: November 2005

ABSTRACTThree aspects of the Anglican understanding of Christianity can make a distinctive contribution to Christian–Muslim dialogue today. Recognition of the importance of context highlights the complexity and variety of the situations in which Christians and Muslims encounter one another. Basing unity on a sense of collegiality which can withstand disagreement offers a model for shared working across religious differences. The interpretation of Scripture through reason and tradition under the guidance of conscience points to a dialogue between those addressed by the Bible and by the Qur'ān respectively. These themes are illustrated through contemporary Anglican involvement in three Christian–Muslim projects, and their theological implications are explored.
Fas ligand is localized to membrane rafts, where it displays increased cell death–inducing activity
Authors: Cahuzac Nathalie , Baum Wiebke , Kirkin Vladimir , Conchonaud Fabien , Wawrezinieck Laure , Marguet Didier , Janssen Ottmar , Zörnig Martin , Hueber Anne-Odile 
Published: November 2005

AbstractFas ligand (FasL), a member of the TNF protein family, potently induces cell death by activating its matching receptor Fas. Fas-mediated killing plays a critical role in naturally and pathologically occurring cell death, including development and homeostasis of the immune system. In addition to its receptor-interacting and cell death–inducing extracellular domain, FasL has a well-conserved intracellular portion with a proline-rich SH3 domain–binding site probably involved in non-apoptotic functions. We report here that, as with the Fas receptor, a fraction of FasL is constitutively localized in rafts. These dynamic membrane microdomains, enriched in sphingolipids and cholesterol, are important for cell signaling and trafficking processes. We show that FasL is partially localized in rafts and that increased amounts of FasL are found in rafts after efficient FasL/Fas receptor interactions. Raft disorganization after cholesterol oxidase treatment and deletions within the intracellular FasL domain diminish raft partitioning and, most important, lead to decreased FasL killing. We conclude that FasL is recruited into lipid rafts for maximum Fas receptor contact and cell death–inducing potency. These findings raise the possibility that certain pathologic conditions may be treated by altering the cell death–inducing capability of FasL with drugs affecting its raft localization.
Mammalian target of rapamycin (mTOR) regulates both proliferation of megakaryocyte progenitors and late stages of megakaryocyte differentiation
Authors: Raslova Hana , Baccini Véronique , Loussaief Lamya , Comba Béatrice , Larghero Jérôme , Debili Najet , Vainchenker William 
Published: November 2005

AbstractA major determinant in platelet production is the megakaryocyte (MK) size that is regulated both by ploidization and the increase in cytoplasmic volume at the end of maturation. Here we investigated the involvement of the mammalian target of rapamycin (mTOR) pathway in the regulation of megakaryopoiesis. We show that phosphorylation of mTOR, p70S6K1, and 4E-BP1 was diminished in thrombopoietin-cultured human MKs after rapamycin treatment. Rapamycin induced an inhibition in the G1/S transition and a decrease in the mean MK ploidy via a diminution of p21 and cyclin D3 occurring at a transcriptional level. Both cycling (2N/4N) and polyploid (8N/16N) MKs were reduced in size, with a size reduction slightly more pronounced in mature polyploid MKs than in immature ones. Rapamycin also induced a delay in the expression of MK markers and prevented the generation of proplatelet MKs. Additional experiments performed in vitro with MKs from mutant mice showed that the decrease in mean ploidy level and the delay in MK differentiation in the presence of rapamycin were less pronounced in CdknIa (p21)–/– MKs than in CdknIa (p21)+/+ MKs. These findings indicate that the mTOR pathway plays an important role during megakaryopoiesis by regulating ploidy, cell size, and maturation, in part by regulating p21 and cyclin D3.
Analysis of transgene-specific immune responses that limit the in vivo persistence of adoptively transferred HSV-TK–modified donor T cells after allogeneic hematopoietic cell transplantation
Authors: Berger Carolina , Flowers Mary E. , Warren Edus H. , Riddell Stanley R. 
Published: November 2005

AbstractThe introduction of an inducible suicide gene such as the herpes simplex virus thymidine kinase (HSV-TK) might allow exploitation of the antitumor activity of donor T cells after allogeneic hematopoietic cell transplantation (HCT) without graft versus host disease. However, HSV-TK is foreign, and immune responses to gene-modified T cells could lead to their premature elimination. We show that after the infusion of HSV-TK–modified donor T cells to HCT recipients, CD8+ and CD4+ T-cell responses to HSV-TK are rapidly induced and coincide with the disappearance of transferred cells. Cytokine flow cytometry using an overlapping panel of HSV-TK peptides allowed rapid detection and quantitation of HSV-TK–specific T cells in the blood and identified multiple immunogenic epitopes. Repeated infusion of modified T cells boosted the induced HSV-TK–specific T cells, which persisted as memory cells. These studies demonstrate the need for nonimmunogenic suicide genes and identify a strategy for detection of CD4+ and CD8+ T-cell responses to transgene products that should be generally applicable to monitoring patients on gene therapy trials. The potency of gene-modified T cells to elicit robust and durable immune responses imply this approach might be used for vaccination to elicit T-cell responses to viral or tumor antigens.
Drug-resistant T-lymphoid tumors undergo apoptosis selectively in response to an antimicrotubule agent, EM011
Authors: Aneja Ritu , Zhou Jun , Vangapandu Surya N. , Zhou Binfei , Chandra Ramesh , Joshi Harish C. 
Published: November 2005

AbstractWe have shown previously that EM011, a synthetic compound, binds tubulin with a higher affinity than the founding compound, noscapine, without changing total microtubule polymer mass. Now we show that EM011 is potently effective against vinblastine-resistant human lymphoblastoid line CEM/VLB100 and its parental vinblastine-sensitive line CEM. The cytotoxicity is mediated by cell cycle arrest at G2/M phase and subsequent apoptosis, as indicated by altered plasma membrane asymmetry, loss of mitochondrial transmembrane potential, activation of caspase-3, and increased DNA fragmentation. Furthermore, oral EM011 treatment of nude mice bearing human lymphoma xenografts results in pronounced tumor regression by triggering apoptosis and significantly lengthens the survival time of mice. EM011 treatment does not have obvious side effects in tissues with frequently dividing cells, such as the spleen and duodenum. In addition, EM011 does not show any toxicity in the liver, lung, heart, brain, and sciatic nerve. More importantly, EM011 does not affect hematopoiesis as determined by complete blood count profiles. These findings suggest that EM011 may be a safe and effective chemotherapeutic agent for oral treatment of drug-resistant human lymphomas. (Blood. 2006;107:2486-2492)
Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
Authors: Chan MCW , Cheung CY , Chui WH , Tsao SW , Nicholls JM , Chan YO , Chan RWY , Long HT , Poon LLM , Guan Y , Peiris JSM 
Published: November 2005

Abstract

Background
Fatal human respiratory disease associated with influenza A subtype H5N1 has been documented in Hong Kong, and more recently in Vietnam, Thailand and Cambodia. We previously demonstrated that patients with H5N1 disease had unusually high serum levels of IP-10 (interferon-gamma-inducible protein-10). Furthermore, when compared with human influenza virus subtype H1N1, the H5N1 viruses in 1997 (A/Hong Kong/483/97) (H5N1/97) were more potent inducers of pro-inflammatory cytokines (e.g. tumor necrosis factor-a) and chemokines (e.g. IP-10) from primary human macrophages in vitro, which suggests that cytokines dysregulation may play a role in pathogenesis of H5N1 disease. Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells.

Methods
We used quantitative RT-PCR and ELISA to compare the profile of cytokine and chemokine gene expression induced by H5N1 viruses A/HK/483/97 (H5N1/97), A/Vietnam/1194/04 and A/Vietnam/3046/04 (both H5N1/04) with that of human H1N1 virus in human primary alveolar and bronchial epithelial cells in vitro.

Results
We demonstrated that in comparison to human H1N1 viruses, H5N1/97 and H5N1/04 viruses were more potent inducers of IP-10, interferon beta, RANTES (regulated on activation, normal T cell expressed and secreted) and interleukin 6 (IL-6) in primary human alveolar and bronchial epithelial cells in vitro. Recent H5N1 viruses from Vietnam (H5N1/04) appeared to be even more potent at inducing IP-10 than H5N1/97 virus.

Conclusion
The H5N1/97 and H5N1/04 subtype influenza A viruses are more potent inducers of proinflammatory cytokines and chemokines in primary human respiratory epithelial cells than subtype H1N1 virus. We suggest that this hyper-induction of cytokines may be relevant to the pathogenesis of human H5N1 disease.
ErmineJ: Tool for functional analysis of gene expression data sets
Authors: Lee Homin K , Braynen William , Keshav Kiran , Pavlidis Paul 
Published: November 2005

AbstractBackgroundIt is common for the results of a microarray study to be analyzed in the context of biologically-motivated groups of genes such as pathways or Gene Ontology categories. The most common method for such analysis uses the hypergeometric distribution (or a related technique) to look for "over-representation" of groups among genes selected as being differentially expressed or otherwise of interest based on a gene-by-gene analysis. However, this method suffers from some limitations, and biologist-friendly tools that implement alternatives have not been reported.ResultsWe introduce ErmineJ, a multiplatform user-friendly stand-alone software tool for the analysis of functionally-relevant sets of genes in the context of microarray gene expression data. ErmineJ implements multiple algorithms for gene set analysis, including over-representation and resampling-based methods that focus on gene scores or correlation of gene expression profiles. In addition to a graphical user interface, ErmineJ has a command line interface and an application programming interface that can be used to automate analyses. The graphical user interface includes tools for creating and modifying gene sets, visualizing the Gene Ontology as a table or tree, and visualizing gene expression data. ErmineJ comes with a complete user manual, and is open-source software licensed under the Gnu Public License.ConclusionThe availability of multiple analysis algorithms, together with a rich feature set and simple graphical interface, should make ErmineJ a useful addition to the biologist's informatics toolbox. ErmineJ is available fromhttp://microarray.cu.genome.org.
Distinct types of glial cells populate the Drosophilaantenna
Authors: Sen Anindya , Shetty Chetak , Jhaveri Dhanisha , Rodrigues Veronica 
Published: November 2005

Abstract

Background
The development of nervous systems involves reciprocal interactions between neurons and glia. In the Drosophila olfactory system, peripheral glial cells arise from sensory lineages specified by the basic helix-loop-helix transcription factor, Atonal. These glia wrap around the developing olfactory axons early during development and pattern the three distinct fascicles as they exit the antenna. In the moth Manduca sexta, an additional set of central glia migrate to the base of the antennal nerve where axons sort to their glomerular targets. In this work, we have investigated whether similar types of cells exist in the Drosophila antenna.

Results
We have used different P(Gal4) lines to drive Green Fluorescent Protein (GFP) in distinct populations of cells within the Drosophila antenna. Mz317::GFP, a marker for cell body and perineural glia, labels the majority of peripheral glia. An additional ~30 glial cells detected by GH146::GFP do not derive from any of the sensory lineages and appear to migrate into the antenna from the brain. Their appearance in the third antennal segment is regulated by normal function of the Epidermal Growth Factor receptor and small GTPases. We denote these distinct populations of cells as Mz317-glia and GH146-glia respectively. In the adult, processes of GH146-glial cells ensheath the olfactory receptor neurons directly, while those of the Mz317-glia form a peripheral layer. Ablation of GH146-glia does not result in any significant effects on the patterning of the olfactory receptor axons.

Conclusion
We have demonstrated the presence of at least two distinct populations of glial cells within the Drosophila antenna. GH146-glial cells originate in the brain and migrate to the antenna along the newly formed olfactory axons. The number of cells populating the third segment of the antenna is regulated by signaling through the Epidermal Growth Factor receptor. These glia share several features of the sorting zone cells described in Manduca.