bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Chronology of Depression and Distress in Men with Peyronie's Disease
Authors: Nelson Christian J. , Diblasio Chris , Kendirci Muammer , Hellstrom Wayne , Guhring Patricia , Mulhall John P. 
Published: June 2008

ABSTRACT

Introduction
For the practicing clinician, appreciating Peyronie's disease (PD) significant negative psychological impact is apparent. Despite this, there exists not a single study using validated instruments assessing this issue.

Aims
To document the effect of PD on the psychosocial status of men.

Main Outcome Measures
The Center for Epidemiological Studies Depression scale (CES-D) for evaluation of depression and the SF-36 for quality of life assessment.

Methods
Men (N = 92; 54 ± 11 years of age) presenting for PD evaluation completed the CES-D, Short Form-36 (SF-36), and an inventory regarding PD. Partners were not assessed.

Results
A vast majority of men (88%) had a partner with a mean partner age of 49 ± 11 years. The median duration of PD at presentation was 12 (1–360) months. As a whole, 48% were classified as depressed on the CES-D (26% moderate, 21% severe). These subjects were then placed into groups according to the length of time since diagnosis of PD. Length-of-time groups were: 0–6 months, 6–12 months, 12–18 months, and &gt;18 months. The percent of men scoring above the CES-D cutoff for depression remained consistently high with no significant difference across time since diagnosis groups. These results are supported by data from the Mental Health subscale (MHS) of the SF-36 (lower scores indicate lower mental heath). For the entire sample, the MHS standardized mean of 46.80 was significantly lower (P &lt; 0.05) than the general male population standardized mean of 50. The MHS means stayed consistently low (no statistical difference) across time since diagnosis groups.

Conclusion
Using validated instruments, we have demonstrated that 48% of men with PD have clinically meaningful depression that would warrant medical evaluation. This high level of depression stayed consistent across time since diagnosis. These data suggest that most men do not psychologically adjust to their diagnosis of PD and all men with PD should be considered appropriate mental health screening.
Tuberous sclerosis with oral fibromatosis: a case report
Authors: Curi M. , Cossolin G. , Koga D. , Silva M. , Zardetto C. 
Published: June 2008

AbstractTuberous sclerosis is an autosomal dominant neurocutaneous syndrome, which may involve multiple organ systems and shows highly variable clinical manifestations. Oral manifestations, including lesions on hard tissues, enamel hypoplasia and gingival hyperplasia have been previously described. We report a case of a 25‐year‐old woman with this syndrome presenting multiple fibrous nodules on the buccal mucosa and lips. Awareness of the different oral manifestations of tuberous sclerosis is important to ensure appropriate diagnosis and treatment.
Mandibular canine transmigration: aethio‐pathogenetic aspects and six new reported cases
Authors: Pippi R. , Kaitsas R. 
Published: June 2008

AbstractMandibular canine transmigration is a rare dental ectopia that creates surgical, orthodontic, restorative and interceptive problems. Aethiologic and pathogenetic aspects of mandibular canine transmigration are examined through an international literature review.Six new cases are then presented. All of them involve the left canine in women aged between 7 and 32 years.Finally, a pathogenetic theory is elaborated to explain such long and lasting tooth migration. Two main factors contribute to this movement: a pericoronal osteolitic area and a strong and lasting ‘vis a tergo’ because of the root formation.An early interceptive treatment of mandibular canine migration can avoid tooth extraction as well as complex orthodontic and restorative therapies.
A case of obstructive sleep apnea syndrome: unusual comorbidity
Authors: Jalladeau E. , Crestani B. , Laissy J.‐P. , Vecchierini M. F. 
Published: June 2008

AbstractIntroduction:  Launois‐Bensaude syndrome (LBS) is a rare disease, characterized by the accumulation of fatty tissue predominantly in the neck, shoulders and thorax, whose diagnostic is clinical.Objectives:  We describe a new case in a 73 year‐old man. As the patient was obese, complained of snoring and was treated for hypertension, we looked for a morbid association with an obstructive sleep apnea syndrome (OSAS).Methods:  A polysomnography (PSG) and a cervical magnetic resonance imaging (MRI) were performed.Results:  PSG demonstrated OSAS with an apnea‐hypopnea index of 43/h. Cervical MRI showed fatty infiltration resulting in airway narrowing at the pharyngeal but not at the tracheal level. Only, 3 cases of such a morbid association have already been published; in 2 of these patients was a tracheal compression. More over a metabolic syndrome was present.Conclusions:  This observation draws attention to the need for seeking OSAS among patients affected by LBS, even in a moderate form, and emphasises the roles of the upper airway narrowing by the fat infiltration as well as the role of the metabolic syndrome in the genesis of OSAS.Please cite this paper as: Jalladeau E, Crestani B, Laissy J‐P and Vecchierini MF. A case of obstructive sleep apnea syndrome: unusual comorbidity. The Clinical Respiratory Journal 2008; 2: 239–241.
Diagnosis of Bovine Paratuberculosis by a Novel Enzyme-Linked Immunosorbent Assay Based on Early Secreted Antigens of
Mycobacterium avium
subsp.
paratuberculosis
Authors: Shin Sung Jae , Cho Donghee , Collins Michael T. 
Published: June 2008

ABSTRACT

We previously reported that protein antigens of serodiagnostic potential were more abundant in culture filtrates than cellular extracts from liquid cultures of
Mycobacterium avium
subsp.
paratuberculosis
(D. Cho and M. T. Collins, Clin. Vaccine Immunol. 13:1155-1161, 2006). Based on this observation, a novel enzyme-linked immunosorbent assay (ELISA) using antigens secreted by young (early- to mid-log-phase) cultures of
M. avium
subsp.
paratuberculosis
JTC303 (a low-passage isolate originating from the ileum of a Holstein bull) in mycobactin-supplemented Watson-Reid medium (pH 6.0) was developed and evaluated using a previously described panel of bovine sera (M. T. Collins et al., Clin. Diagn. Lab. Immunol. 12:685-692, 2005) that included 444 paratuberculosis cases and 412 controls. The new assay, called JTC-ELISA, had a significantly higher diagnostic sensitivity and an equivalent specificity compared to those of five commercial paratuberculosis ELISA kits. By receiver-operating characteristic analysis, the JTC-ELISA had the highest area under the curve of the six assays evaluated. The JTC-ELISA was particularly sensitive at detecting low-level fecal shedders of
Mavium
subsp.
paratuberculosis
(40%; the sensitivity of the commercial kits was 20%). The JTC-ELISA works effectively on both serum and milk samples for the detection of cattle with subclinical
M. avium
subsp.
paratuberculosis
infections, providing a cost-effective diagnostic tool to support paratuberculosis control programs in cattle herds.
Comparative Testing of Six Antigen-Based Malaria Vaccine Candidates Directed Toward Merozoite-StagePlasmodium falciparum
Authors: Arnot David E. , Cavanagh David R. , Remarque Edmond J. , Creasey Alison M. , Sowa Mercy P. K. , Morgan William D. , Holder Anthony A. , Longacre Shirley , Thomas Alan W. 
Published: June 2008

ABSTRACTImmunogenicity testing ofPlasmodium falciparumantigens being considered as malaria vaccine candidates was undertaken in rabbits. The antigens compared were recombinant baculovirus MSP-119and fivePichia pastoriscandidates, including two versions of MSP-119, AMA-1 (domains I and II), AMA-1+MSP-119, and fused AMA-1/MSP-119). Animals were immunized with equimolar amounts of each antigen, formulated in Montanide ISA720. The specificities and titers of antibodies were compared using immunofluorescence assays and enzyme-linked immunosorbent assay (ELISA). The antiparasite activity of immunoglobulin G (IgG) in in vitro cultures was determined by growth inhibition assay, flow cytometry, lactate dehydrogenase assay, and microscopy. Baculovirus MSP-119immunizations produced the highest parasite-specific antibody titers in immunofluorescence assays. In ELISAs, baculovirus-produced MSP-119induced more antibodies than any other single MSP-119immunogen and three times more MSP-119specific antibodies than the AMA-1/MSP-119fusion. Antibodies induced by baculovirus MSP-119gave the highest levels of growth inhibition in HB3 and 3D7 parasite cultures, followed by AMA-1+MSP-119and the AMA-1/MSP-119fusion. With the FCR3 isolate (homologous to the AMA-1 construct), antibodies to the three AMA-1-containing candidates gave the highest levels of growth inhibition at high IgG concentrations, but antibodies to baculovirus MSP-119inhibited as well or better at lower IgG concentrations. The twoP. pastoris-produced MSP-119-induced IgGs conferred the lowest growth inhibition. Comparative analysis of immunogenicity of vaccine antigens can be used to prioritize candidates before moving to expensive GMP production and clinical testing. The assays used have given discriminating readouts but it is not known whether any of them accurately reflect clinical protection.
Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains ofEscherichia coli
Authors: Hill Joshua , Beriwal Shilpa , Chandra Ishwad , Paul Vinod K. , Kapil Aarti , Singh Tripti , Wadowsky Robert M. , Singh Vinita , Goyal Ankur , Jahnukainen Timo , Johnson James R. , Tarr Phillip I. , Vats Abhay 
Published: June 2008

ABSTRACTWe developed a highly sensitive and specific LAMP assay forEscherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36E. coliisolates and all 22 urine samples (out of 89 samples tested) that hadE. coli. This assay is rapid, low in cost, and simple to perform.
Discriminating between Varicella-Zoster Virus Vaccine and Wild-Type Strains by Loop-Mediated Isothermal Amplification
Authors: Higashimoto Yuki , Ihira Masaru , Ohta Akane , Inoue Shigeki , Usui Chie , Asano Yoshizo , Yoshikawa Tetsushi 
Published: June 2008

ABSTRACT
The loop-mediated isothermal amplification (LAMP) method was developed to distinguish between the varicella-zoster virus (VZV) vaccine (vOka) strain and wild-type strains. Two single nucleotide polymorphisms (SNPs) (nucleotide [nt] 105705 for VR-1 VZV LAMP and nt 106262 for VR-2 VZV LAMP) located in the open reading frame 62 gene were selected as LAMP targets. Amplified vOka DNA demonstrated a typical ladder pattern; however, no LAMP product was detected in reactions performed with DNAs from other human herpesviruses by either VR-1 VZV LAMP or VR-2 VZV LAMP. This result was confirmed by a turbidity assay. The sensitivities of both VR-1 and VR-2 VZV LAMP determined by either the turbidity assay or agarose gel electrophoresis were 100 copies per reaction. To discriminate the vOka strain from wild-type strains, VR-1 and VR-2 VZV LAMP products were digested with the appropriate restriction enzymes (SacII for VR-1 LAMP and SmaΙ for VR-2 LAMP). The digested products were clearly different in the vOka strain and wild-type strains. To evaluate the utility of the LAMP methods for rapid differentiation, viral DNA (without DNA extraction) in swab samples was directly tested. Wild-type VZV DNA was detected in 20 swab samples by either VR-1 VZV LAMP or VR-2 VZV LAMP. Sequence analysis confirmed the expected SNPs in the LAMP products amplified from the vOka strain and the five wild-type strains.
Assessment of Cryptodiag for Diagnosis of Cryptosporidiosis and Genotyping
Cryptosporidium
Species
Authors: Savin C. , Sarfati C. , Menotti J. , Jaouhari J. , Wurtzer S. , Garin Y. J. F. , Derouin F. 
Published: June 2008

ABSTRACT

The performance of a new commercial PCR-enzyme-linked immunosorbent assay (ELISA) (Cryptodiag; Bio Advance, France) for the diagnosis of cryptosporidiosis and the identification of
Cryptosporidium hominis
and
C. parvum
from stool samples was examined. This test is based on PCR amplification of
Cryptosporidium
DNA extracted from stools, followed by an ELISA based on hybridization with
Cryptosporidium
sp.-,
C. hominis
-, or
C. parvum
-specific probes. In spiking experiments, approximately five oocysts were detected either in water or in stool suspensions while assessing for the efficient removal of stool PCR inhibitors. No cross-reactivity was observed in the detection of
C. parvum
and
C. homin
is using the respective specific probes. Thirty-three fecal samples from patients with microscopically proven cryptosporidiosis and 118 from patients with or without other digestive protozoan infections were tested by Cryptodiag, blinded to the results of microscopy. Compared to microscopy, the sensitivity of Cryptodiag was 97.0% (32/33) and 100% (33/33), including the gray zone, and specificity was 98.3% (116/118) and 96.6% (114/118), including the gray zone. Among 34 positive results, Cryptodiag identified 19 due to
C. hominis
, 8 due to
C. parvum
, and 7 due to
Cryptosporidium
spp. Genotyping by Cryptodiag agreed with reference typing methods in 85% of cases of
C. parvum
or
C. hominis
infections. Cryptodiag proved to be reliable and sensitive for the diagnosis of cryptosporidiosis. The use of specific probes allowed the identification of
C. hominis
and
C. parvum
, i.e., the two main species responsible for human cryptosporidiosis, and rapidly provided information on the possible source of infection.
Multicenter Comparison of PCR Assays for Detection of Human Herpesvirus 6 DNA in Serum
Authors: Flamand Louis , Gravel Annie , Boutolleau David , Alvarez-Lafuente Roberto , Jacobson Steve , Malnati Mauro S. , Kohn Debra , Tang Yi-Wei , Yoshikawa Tetsushi , Ablashi Dharam 
Published: June 2008

ABSTRACT
Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The lack of a standardized assay that can be used to detect and estimate HHV-6 DNA contents in various clinical specimens can lead and has led to discordant results among investigators and on the potential association of HHV-6 to diseases. To identify the most reliable and sensitive assays, an identical set of 11 coded serum samples spiked with various quantities of the HHV-6A variant (range, 4 to 400,000 genome copies/ml) was sent to eight independent laboratories around the world. Each laboratory was asked to estimate the HHV-6 DNA content by use of its own protocols and assays. Among the various assays, three TaqMan-based real-time PCR assays yielded quantities that were closest to the quantity of HHV-6 that had been spiked. To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays.
Extended-Spectrum-Beta-Lactamase Production in a
Salmonella enterica
Serotype Typhi Strain from the Philippines
Authors: Al Naiemi Nashwan , Zwart Bastiaan , Rijnsburger Martine C. , Roosendaal Robert , Debets-Ossenkopp Yvette J. , Mulder Janet A. , Fijen Cees A. , Maten Willemina , Vandenbroucke-Grauls Christina M. , Savelkoul Paul H. 
Published: June 2008

ABSTRACT

A
Salmonella enterica
serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase.
Association between 16S-23S Internal Transcribed Spacer Sequence Groups of
Mycobacterium avium
Complex and Pulmonary Disease
Authors: Stout Jason E. , Hopkins Gregory W. , McDonald Jay R. , Quinn Anita , Hamilton Carol D. , Reller L. Barth , Frothingham Richard 
Published: June 2008

ABSTRACT

Organisms within the
Mycobacterium avium
complex (MAC) may have differential virulence. We compared 33 subjects with MAC pulmonary disease to 75 subjects with a single positive culture without disease.
M. avium
isolates were significantly more likely to be associated with MAC pulmonary disease (odds ratio = 5.14, 95% confidence interval = 1.25 to 22.73) than
M. intracellulare
.
Gram Stain-Specific-Probe-Based Real-Time PCR for Diagnosis and Discrimination of Bacterial Neonatal Sepsis
Authors: Wu Yi-Dong , Chen Li-Hua , Wu Xiu-Jing , Shang Shi-Qiang , Lou Jin-Tu , Du Li-Zhong , Zhao Zheng-Yan 
Published: June 2008

ABSTRACT

Sepsis is a serious disease with high mortality in newborns. It is very important to have a convenient and accurate method for pathogenic diagnosis of neonatal sepsis. We developed a method of simultaneous detection and Gram classification of clinically relevant bacterial pathogens causing sepsis directly from blood samples with Gram stain-specific-probe-based real-time PCR (GSPBRT-PCR). With GSPBRT-PCR, 53 clinically important strains representing 25 gram-positive and 28 gram-negative bacterial species were identified correctly with the corresponding Gram probe. The limits of the GSPBRT-PCR assay in serial dilutions of the bacteria revealed that
Staphylococcus aureus
could be detected at concentrations of 3 CFU per PCR and
Escherichia coli
at concentrations as low as 1 CFU per PCR. The GSPBRT-PCR assay was further evaluated on 600 blood specimens from patients with suspicioon of neonatal sepsis and compared to the results obtained from blood cultures. The positive rate of the GSPBRT-PCR array was 50/600 (8.33%), significantly higher than that of blood culture (34/600; 5.67%) (
P
= 0.00003). When blood culture was used as a control, the sensitivity of GSPBRT-PCR was 100%, the specificity was 97.17%, and the index of accurate diagnosis was 0.972. This study suggests that GSPBRT-PCR is very useful for the rapid and accurate diagnosis of bacterial infection and that it can have an important impact on the current inappropriate and unnecessary use of antibiotics in the treatment of newborns.
Effective Chemokine Secretion by Dendritic Cells and Expansion of Cross-Presenting CD4−/CD8+Dendritic Cells Define a Protective Phenotype in the Mouse Model of Coxsackievirus Myocarditis
Authors: Weinzierl Andreas Oliver , Szalay Gudrun , Wolburg Hartwig , Sauter Martina , Rammensee Hans-Georg , Kandolf Reinhard , Stevanović Stefan , Klingel Karin 
Published: June 2008

ABSTRACTEnteroviruses such as coxsackievirus B3 (CVB3) are able to induce lethal acute and chronic myocarditis. In resistant C57BL/6 mice, CVB3 myocarditis is abrogated by T-cell-dependent mechanisms, whereas major histocompatibility complex (MHC)-matched permissive A.BY/SnJ mice develop chronic myocarditis based on virus persistence. To define the role of T-cell-priming dendritic cells (DCs) in the outcome of CVB3 myocarditis, DCs were analyzed in this animal model in the course of CVB3 infection. In both mouse strains, DCs were found to be infectible with CVB3; however, formation of infectious virions was impaired. In DCs derived from C57BL/6 mice, significantly higher quantities of interleukin-10 (IL-10) and the proinflammatory cytokines IL-6 and tumor necrosis factor alpha were measured compared to those from A.BY/SnJ mice. Additionally, the chemokines interferon-inducible protein 10 (IP-10) and RANTES were secreted by DCs from resistant C57BL/6 mice earlier in infection and at significantly higher levels. The protective role of IP-10 in CVB3 myocarditis was confirmed in IP-10−/−mice, which had increased myocardial injury compared to the immunocompetent control animals. Also, major differences in resistant and permissive mice were found in DC subsets, with C57BL/6 mice harboring more cross-priming CD4−CD8+DCs. As CD4−CD8+DCs are known to express 10 times more Toll-like receptor 3 (TLR3) than other DC subsets, we followed the course of CVB3 infection in TLR3−/−mice. These mice developed a fulminant acute myocarditis and secreted sustained low amounts of type I interferons; secretion of IP-10 and RANTES was nearly abrogated in DCs. We conclude that MHC-independent genetic factors involving DC-related IP-10 secretion and TLR3 expression are beneficial in the prevention of chronic coxsackievirus myocarditis.
Sf29
Gene of
Spodoptera frugiperda
Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies
Authors: Simón Oihane , Williams Trevor , Asensio Aaron C. , Ros Sarhay , Gaya Andrea , Caballero Primitivo , Possee Robert D. 
Published: June 2008

ABSTRACT

The genome of
Spodoptera frugiperda
multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in
S. frugiperda
. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were equally infectious when used to transfect
S. frugiperda
. All three viruses produced similar numbers of OBs, although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with Sf29null bacmid died ∼24 h later than positive controls, consistent with the reduced virus particle content of Sf29null OBs. Transcripts from
Sf29
were detected in infected insects 12 h prior to those from the polyhedrin gene. Homologs to
Sf29
were present in other group II NPVs, and similar sequences were present in entomopoxviruses. Analysis of the
Sf29
predicted protein sequence revealed signal peptide and transmembrane domains, but the presence of 12 potential N
-
glycosylation sites suggest that it is not an ODV envelope protein. Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions. We conclude that
Sf29
is a viral factor that determines the number of ODVs occluded in each OB.
The Envelope of Intracellular African Swine Fever Virus Is Composed of a Single Lipid Bilayer
Authors: Hawes Philippa C. , Netherton Christopher L. , Wileman Thomas E. , Monaghan Paul 
Published: June 2008

ABSTRACT

African swine fever virus (ASFV) is a member of a family of large nucleocytoplasmic DNA viruses that include poxviruses, iridoviruses, and phycodnaviruses. Previous ultrastructural studies of ASFV using chemical fixation and cryosectioning for electron microscopy (EM) have produced uncertainty over whether the inner viral envelope is composed of a single or double lipid bilayer. In this study we prepared ASFV-infected cells for EM using chemical fixation, cryosectioning, and high-pressure freezing. The appearance of the intracellular viral envelope was determined and compared to that of mitochondrial membranes in each sample. The best resolution of membrane structure was obtained with samples prepared by high-pressure freezing, and images suggested that the envelope of ASFV consisted of a single lipid membrane. It was less easy to interpret virus structure in chemically fixed or cryosectioned material, and in the latter case the virus envelope could be interpreted as having two membranes. Comparison of membrane widths in all three preparations indicated that the intracellular viral envelope of ASFV was not significantly different from the outer mitochondrial membrane (
P
< 0.05). The results support the hypothesis that the intracellular ASFV viral envelope is composed of a single lipid bilayer.