bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three-Way Error Analysis between AATSR, AMSR-E, and In Situ Sea Surface Temperature Observations
Authors: O’Carroll Anne G. , Eyre John R. , Saunders Roger W. 
Published: July 2008

AbstractUsing collocations of three different observation types of sea surface temperatures (SSTs) gives enough information to enable the standard deviation of error on each observation type to be derived. SSTs derived from the Advanced Along-Track Scanning Radiometer (AATSR) and Advanced Microwave Scanning Radiometer for Earth Observing System (EOS; AMSR-E) instruments are used, along with SST observations from buoys. Various assumptions are made within the error theory, including that the errors are not correlated, which should be the case for three independent data sources. An attempt is made to show that this assumption is valid and that the covariances between the different observations because of representativity error are negligible. Overall, the spatially averaged nighttime AATSR dual-view three-channel bulk SST observations for 2003 are shown to have a very small standard deviation of error of 0.16 K, whereas the buoy SSTs have an error of 0.23 K and the AMSR-E SST observations have an error of 0.42 K.
Explorations of Atmosphere–Ocean–Ice Climates on an Aquaplanet and Their Meridional Energy Transports
Authors: Enderton Daniel , Marshall John 
Published: July 2008

Abstract
The degree to which total meridional heat transport is sensitive to the details of its atmospheric and oceanic components is explored. A coupled atmosphere, ocean, and sea ice model of an aquaplanet is employed to simulate very different climates—some with polar ice caps, some without—even though they are driven by the same incoming solar flux. Differences arise due to varying geometrical constraints on ocean circulation influencing its ability to transport heat meridionally. Without complex land configurations, the results prove easier to diagnose and compare to theory and simple models and, hence, provide a useful test bed for ideas about heat transport and its partition within the climate system. In particular, the results are discussed in the context of a 1978 study by Stone, who argued that for a planet with Earth’s astronomical parameters and rotation rate, the total meridional heat transport would be independent of the detailed dynamical processes responsible for that transport and depend primarily on the distribution of incoming solar radiation and the mean planetary albedo. The authors find that in warm climates in which there is no ice, Stone’s result is a useful guide. In cold climates with significant polar ice caps, however, meridional gradients in albedo significantly affect the absorption of solar radiation and need to be included in any detailed calculation or discussion of total heat transport. Since the meridional extent of polar ice caps is sensitive to details of atmospheric and oceanic circulation, these cannot be ignored. Finally, what has been learned is applied to a study of the total heat transport estimated from the Earth Radiation Budget Experiment (ERBE) data.
Error Growth in a Whole Atmosphere Climate Model
Authors: Liu H-L. , Sassi F. , Garcia R. R. 
Published: July 2008

Abstract
It has been well established that the atmosphere is chaotic by nature and thus has a finite limit of predictability. The chaotic divergence of initial conditions and the predictability are explored here in the context of the whole atmosphere (from the ground to the thermosphere) using the NCAR Whole Atmosphere Community Climate Model (WACCM). From ensemble WACCM simulations, it is found that the early growth of differences in initial conditions is associated with gravity waves and it becomes apparent first in the upper atmosphere and progresses downward. The differences later become more profound on increasingly larger scales, and the growth rates of the differences change in various atmospheric regions and with seasons—corresponding closely with the strength of planetary waves. For example, in December–February the growth rates are largest in the northern and southern mesosphere and lower thermosphere and in the northern stratosphere, while smallest in the southern stratosphere. The growth rates, on the other hand, are not sensitive to the altitude where the small differences are introduced in the initial conditions or the physical nature of the differences. Furthermore, the growth rates in the middle and upper atmosphere are significantly reduced if the lower atmosphere is regularly reinitialized, and the reduction depends on the frequency and the altitude range of the reinitialization.
Two Dynamical Core Formulation Flaws Exposed by a Baroclinic Instability Test Case
Authors: Williamson David L. , Olson Jerry G. , Jablonowski Christiane 
Published: July 2008

Abstract
Two flaws in the semi-Lagrangian algorithm originally implemented as an optional dynamical core in the NCAR Community Atmosphere Model (CAM3.1) are exposed by steady-state and baroclinic instability test cases. Remedies are demonstrated and have been incorporated in the dynamical core. One consequence of the first flaw is an erroneous damping of the speed of a zonally uniform zonal wind undergoing advection by a zonally uniform zonal flow field. It results from projecting the transported vector wind expressed in unit vectors at the arrival point to the surface of the sphere and is eliminated by rotating the vector to be parallel to the surface. The second flaw is the formulation of an a posteriori energy fixer that, although small, systematically affects the temperature field and leads to an incorrect evolution of the growing baroclinic wave. That fixer restores the total energy at each time step by changing the provisional forecast temperature proportionally to the magnitude of the temperature change at that time step. Two other fixers are introduced that do not exhibit the flaw. One changes the provisional temperature everywhere by an additive constant, and the other changes it proportionally by a multiplicative constant.
The lateral mobility of cell adhesion molecules is highly restricted at septate junctions in Drosophila
Authors: Laval Monique , Bel Christophe , Faivre-Sarrailh Catherine 
Published: July 2008

Abstract

Background
A complex of three cell adhesion molecules (CAMs) Neurexin IV(Nrx IV), Contactin (Cont) and Neuroglian (Nrg) is implicated in the formation of septate junctions between epithelial cells in Drosophila. These CAMs are interdependent for their localization at septate junctions and e.g. null mutation of nrx IV or cont induces the mislocalization of Nrg to the baso-lateral membrane. These mutations also result in ultrastructural alteration of the strands of septate junctions and breakdown of the paracellular barrier. Varicose (Vari) and Coracle (Cora), that both interact with the cytoplasmic tail of Nrx IV, are scaffolding molecules required for the formation of septate junctions.

Results
We conducted photobleaching experiments on whole living Drosophila embryos to analyze the membrane mobility of CAMs at septate junctions between epithelial cells. We show that GFP-tagged Nrg and Nrx IV molecules exhibit very stable association with septate junctions in wild-type embryos. Nrg-GFP is mislocalized to the baso-lateral membrane in nrx IV or cont null mutant embryos, and displays increased mobile fraction. Similarly, Nrx IV-GFP becomes distributed to the baso-lateral membrane in null mutants of vari and cora, and its mobile fraction is strongly increased. The loss of Vari, a MAGUK protein that interacts with the cytoplasmic tail of Nrx IV, has a stronger effect than the null mutation of nrx IV on the lateral mobility of Nrg-GFP.

Conclusion
The strands of septate junctions display a stable behavior in vivo that may be correlated with their role of paracellular barrier. The membrane mobility of CAMs is strongly limited when they take part to the multimolecular complex forming septate junctions. This restricted lateral diffusion of CAMs depends on both adhesive interactions and clustering by scaffolding molecules. The lateral mobility of CAMs is strongly increased in embryos presenting alteration of septate junctions. The stronger effect of vari by comparison with nrx IV null mutation supports the hypothesis that this scaffolding molecule may cross-link different types of CAMs and play a crucial role in stabilizing the strands of septate junctions.
Childhood TB epidemiology and treatment outcomes in Thailand: a TB active surveillance network, 2004 to 2006
Authors: Lolekha Rangsima , Anuwatnonthakate Amornrat , Nateniyom Sriprapa , Sumnapun Surin , Yamada Norio , Wattanaamornkiat Wanpen , Sattayawuthipong Wanchai , Charusuntonsri Pricha , Sanguanwongse Natpatou , Wells Charles D , Varma Jay K 
Published: July 2008

Abstract

Background
Of the 9.2 million new TB cases occurring each year, about 10% are in children. Because childhood TB is usually non-infectious and non-fatal, national programs do not prioritize childhood TB diagnosis and treatment. We reviewed data from a demonstration project to learn more about the epidemiology of childhood TB in Thailand.

Methods
In four Thai provinces and one national hospital, we contacted healthcare facilities monthly to record data about persons diagnosed with TB, assist with patient care, provide HIV counseling and testing, and obtain sputum for culture and susceptibility testing. We analyzed clinical and treatment outcome data for patients age < 15 years old registered in 2005 and 2006.

Results
Only 279 (2%) of 14,487 total cases occurred in children. The median age of children was 8 years (range: 4 months, 14 years). Of 197 children with pulmonary TB, 63 (32%) were bacteriologically-confirmed: 56 (28%) were smear-positive and 7 (4%) were smear-negative, but culture-positive. One was diagnosed with multi-drug resistant TB. HIV infection was documented in 75 (27%). Thirteen (17%) of 75 HIV-infected children died during TB treatment compared with 4 (2%) of 204 not known to be HIV-infected (p < 0.01).

Conclusion
Childhood TB is infrequently diagnosed in Thailand. Understanding whether this is due to absence of disease or diagnostic effort requires further research. HIV contributes substantially to the childhood TB burden in Thailand and is associated with high mortality.
AFP, PIVKAII, GP3, SCCA-1 and follisatin as surveillance biomarkers for hepatocellular cancer in non-alcoholic and alcoholic fatty liver disease
Authors: Beale Gary , Chattopadhyay Dipankar , Gray Joe , Stewart Stephen , Hudson Mark , Day Christopher , Trerotoli Paolo , Giannelli Gianluigi , Manas Derek , Reeves Helen 
Published: July 2008

Abstract

Background
The incidence and mortality of hepatocellular cancer (HCC) complicating alcoholic and non-alcoholic fatty liver diseases (ALD and NAFLD) is rising in western societies. Despite knowing the at risk populations for HCC development, the lack of sensitive and specific means of surveillance hampers disease detection at curable stages. The most widely used serum HCC marker is alpha-fetoprotein (AFP), while PIVKA-II, glypican-3 (GP3) and Squamous Cell Carcinoma Antigen -1 (SCCA-1) have been proposed as new biomarkers. Assessment of these HCC biomarkers has largely been performed in patients with viral hepatitis. We conducted a cross sectional study assessing the value of these serum proteins, as well a novel candidate biomarker -follistatin – in patients with HCC arising on a background of ALD or NAFLD.

Methods
Pre-treatment serum samples from 50 patients with HCC arising on a background of ALD (n = 31) or NAFLD (n = 19) were assessed by specific ELISA assay for PIVKAII, Glypican-3, SCCA-1 and Follistatin. Results were compared and contrasted with a control patient group with biopsy proven steatohepatitis-related cirrhosis (n = 41). The diagnostic accuracy of each of the candidate biomarkers was evaluated using receiver operating characteristic (ROC) curve analysis, reporting the area under the curve (AUC) and its 95% confidence interval (CI). Performance was compared to that of the established biomarker, AFP.

Results
Serum levels of all proteins were assessed by specific ELISA assays. GP3, SCCA-1 and follistatin had no HCC surveillance benefit in these patients. AFP and PIVKAII were superior to the other markers, particularly in combination.

Conclusion
We conclude that while novel means of surveillance are urgently required, the combination of AFP and PIVKAII for HCC is an improvement on AFP alone in ALD/NAFLD patients. Furthermore, our data in this homogenous subset of patients- particularly that confirming no role for SCCA-1 – suggests that the choice of optimal biomarkers for HCC surveillance may be determined by the aetiology of underlying chronic liver disease.
The Sertindole Safety Survey: A retrospective analysis under a named patient use programme in Europe
Authors: Lançon Christophe , Toumi Mondher , Sapin Christophe , Hansen Karina 
Published: July 2008

AbstractBackgroundAfter sertindole's suspension, health authorities established a specific named-patient use (NPU) programme in order to supply sertindole to patients who did not respond to or did not tolerate alternative treatments. This programme provided the possibility of prospectively following an exhaustive cohort of patients treated with sertindole after its suspension. A survey was performed to assess sertindole's modalities of prescription, assess and document any serious adverse events (SAEs), and assess the mortality rate within the NPU cohort.MethodsThe study comprised a survey of sertindole-treated patients in eleven European countries. All patients treated with sertindole within the NPU programme were eligible for the study.Results1,432 patients were included in the study. The reason for sertindole prescription was lack of efficacy (approximately 50%) or adverse events (approximately 20%) of other antipsychotic treatments. The mean sertindole dose was 13.4 mg daily. Lack of efficacy and adverse events were reported as reasons for sertindole discontinuation.A total of 97 SAEs were recorded, including ten fatal outcomes, which occurred during the study period or within thirty days after sertindole discontinuation. The all-cause mortality rate was 0.51 per 100 Person-Years of Exposure (95% Poisson confidence interval: 0.23–0.97). QTc prolongation was reported in 15 patients (1.05% of total patients), being a rate of 0.85 per 100 Person-Years of Exposure [95% CI: 0.48–1.41].ConclusionAlthough prescribing and supplying sertindole were subject to administrative constraints, a significant number of patients were treated with sertindole, thus supporting the need for sertindole in specific cases.Trial registration numberNot applicable.
Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells
Authors: Vacharaksa Anjalee , Asrani Anil C , Gebhard Kristin H , Fasching Claudine E , Giacaman Rodrigo A , Janoff Edward N , Ross Karen F , Herzberg Mark C 
Published: July 2008

Abstract

Background
Oral keratinocytes on the mucosal surface are frequently exposed to HIV-1 through contact with infected sexual partners or nursing mothers. To determine the plausibility that oral keratinocytes are primary targets of HIV-1, we tested the hypothesis that HIV-1 infects oral keratinocytes in a restricted manner.

Results
To study the fate of HIV-1, immortalized oral keratinocytes (OKF6/TERT-2; TERT-2 cells) were characterized for the fate of HIV-specific RNA and DNA. At 6 h post inoculation with X4 or R5-tropic HIV-1, HIV-1gag RNA was detected maximally within TERT-2 cells. Reverse transcriptase activity in TERT-2 cells was confirmed by VSV-G-mediated infection with HIV-NL4-3Δenv-EGFP. AZT inhibited EGFP expression in a dose-dependent manner, suggesting that viral replication can be supported if receptors are bypassed. Within 3 h post inoculation, integrated HIV-1 DNA was detected in TERT-2 cell nuclei and persisted after subculture. Multiply spliced and unspliced HIV-1 mRNAs were not detectable up to 72 h post inoculation, suggesting that HIV replication may abort and that infection is non-productive. Within 48 h post inoculation, however, virus harbored by CD4 negative TERT-2 cells trans infected co-cultured peripheral blood mononuclear cells (PBMCs) or MOLT4 cells (CD4+ CCR5+) by direct cell-to-cell transfer or by releasing low levels of infectious virions. Primary tonsil epithelial cells also trans infected HIV-1 to permissive cells in a donor-specific manner.

Conclusion
Oral keratinocytes appear, therefore, to support stable non-replicative integration, while harboring and transmitting infectious X4- or R5-tropic HIV-1 to permissive cells for up to 48 h.
FISH mapping of Philadelphia negative BCR/ABL1 positive CML
Authors: Virgili Anna , Brazma Diana , Reid Alistair G , Howard-Reeves Julie , Valgañón Mikel , Chanalaris Anastasios , De Melo Valeria AS , Marin David , Apperley Jane F , Grace Colin , Nacheva Ellie P 
Published: July 2008

Abstract

Background
Chronic myeloid leukaemia (CML) is a haematopoietic stem cell disorder, almost always characterized by the presence of the Philadelphia chromosome (Ph), usually due to t(9;22)(q34;q11) or its variants. The Ph results in the formation of the BCR/ABL1 fusion gene, which is a constitutively activated tyrosine kinase. Around 1% of CML patients appear to have a Ph negative karyotype but carry a cryptic BCR/ABL1 fusion that can be located by fluorescence in situ hybridisation (FISH) at chromosome 22q11, 9q34 or a third chromosome. Here we present FISH mapping data of BCR and ABL1 flanking regions and associated chromosomal rearrangements in 9 Ph negative BCR/ABL1 positive CML patients plus the cell line CML-T1.

Results

BCR/ABL1 was located at 9q34 in 3 patients, 22q11 in 5 patients and CML-T1 and 22p11 in 1 patient. In 3 of 6 cases with the fusion at 22q11 a distal breakpoint cluster was found within a 280 Kb region containing the RAPGEF1 gene, while in another patient and the CML-T1 the distal breakpoint fell within a single BAC clone containing the 3' RXRA gene. Two cases had a duplication of the masked Ph while genomic deletions of the flanking regions were identified in 3 cases. Even more complex rearrangements were found in 3 further cases.

Conclusion

BCR/ABL1 formation resulted from a direct insertion (one step mechanism) in 6 patients and CML-T1, while in 3 patients the fusion gene originated from a sequence of rearrangements (multiple steps). The presence of different rearrangements of both 9q34 and 22q11 regions highlights the genetic heterogeneity of this subgroup of CML. Future studies should be performed to confirm the presence of true breakpoint hot spots and assess their implications in Ph negative BCR/ABL1 positive CML.
Paraganglioma-like dermal melanocytic tumor: a case report
Authors: Sarma Deba P , Teruya Bryan , Wang Bo 
Published: July 2008

Abstract
Paraganglioma-like dermal melanocytic tumor is a rare subtype of benign dermal melanocytic tumors. Its histopathologic features resemble those of paraganglioma, but the immunostaining characteristics are those of melanocytic lesions.
We report a case of a 60-year-old male with a paraganglioma-like dermal melanocytic tumor of his left cheek and briefly review the English literature.
The History and Development of the Human Genetics Society of Australasia
Authors: Sutherland Grant R. 
Published: July 2008

AbstractThe Human Genetics Society of Australasia is a vibrant professional society with more than 900 members that promotes and regulates the practice of human and medical genetics in Australia and New Zealand. The growth of human genetics was stimulated by the development of diagnostic clinical cytogenetics laboratories in the early to mid 1960s. This coincided with the recognition by medical specialists, mainly pediatricians, that genetic disorders, especially inborn errors of metabolism and birth defects, were of clinical interest and potentially challenging areas for their skills. The organization of professionals in human genetics was slow to evolve. There was an early Western Australian Human Genetics Society, and the cytogenetics community had begun to meet annually from about 1966 but was coordinated by a mailing list rather than as a formal organization. In 1976, as part of the celebrations of the Centenary Year of the Adelaide Children's Hospital, a clinical genetics meeting involving several high profile international speakers and most of the senior medical geneticists in Australia and New Zealand along with the annual meeting of the loose-knit cytogeneticists group agreed that a small working group be charged with setting up a Human Genetics Society. The society was formally incorporated in South Australia in 1977.
After Partial Knee Replacement, Patients Can Kneel, But They Need to Be Taught to Do So: A Single-Blind Randomized Controlled Trial
Authors: Jenkins Cathy , Barker Karen L , Pandit Hemant , Dodd Christopher AF , Murray David W 
Published: July 2008

Background and PurposeKneeling is an important functional activity frequently not performed after knee replacement, thus affecting a patient's ability to carry out basic daily tasks. Despite no clinical reason preventing kneeling, many patients fail to resume this activity. The purpose of this study was to determine whether a single physical therapy intervention would improve patient-reported kneeling ability following partial knee replacement (PKR).SubjectsSixty adults with medial compartment osteoarthritis, suitable for a PKR, participated.MethodsThis was a single-blind, prospective randomized controlled trial. Six weeks after PKR, participants randomly received either kneeling advice and education or routine care where no specific kneeling advice was given. Reassessment was at 1 year postoperatively. The primary outcome measure was patient-reported kneeling ability, as assessed by question 7 of the Oxford Knee Score. Other factors associated with kneeling ability were recorded. These factors were scar position, numbness, range of flexion, involvement of other joints, and pain. Statistical analysis included nonparametric tests and binary logistic regression.ResultsA significant improvement in patient-reported kneeling ability was found at 1 year postoperatively in those participants who received the kneeling intervention. Group allocation was the only factor determining an improvement in patient-reported kneeling ability at 1 year postoperatively.Discussion and ConclusionThe single factor that predicted patient-reported kneeling ability at 1 year postoperatively was the physical therapy kneeling intervention given at 6 weeks after PKR. The results of this study suggest that advice and instruction in kneeling should form part of a postoperative rehabilitation program after PKR. The results can be applied only to patients following PKR.