bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agreement between routinely used immunoassays for thyroid function testing in non‐pregnant and pregnant adults
Authors: Wigh Ida Marie Nørum , Andersen Lærke , Lundgaard Maja Hjelm , Torp Nanna Maria Uldall , Karmisholt Jesper , Andersen Stig , Andersen Stine Linding 
Published: April 2024

AbstractObjectiveThyroid function tests are common biochemical analyses, and agreement between the routinely used immunoassays is important for diagnosis and monitoring of thyroid disease. Efforts are continuously made to align the biochemical assays, and we aimed to evaluate the agreement between immunoassays used in a clinical laboratory setting among non‐pregnant and pregnant adults.DesignCross‐sectional study.ParticipantsSerum samples were obtained from 192 blood donors (non‐pregnant adults) and from 86 pregnant women in the North Denmark Region with no known thyroid disease.MeasurementsEach sample was used for measurement of thyroid‐stimulating hormone (TSH) with the routinely used automatic immunoassays in the regional Departments of Clinical Biochemistry (Alinity, Abbott Laboratories, Cobas, Roche Diagnostics, and Atellica, Siemens Healthineers) and reported as the median with 95% confidence interval (95% CI).ResultsIn nonpregnant adults, the level of TSH was higher with Cobas and Atellica than with Alinity as reflected by median (Alinity: 1.39 mIU/L (95% CI: 1.30–1.51 mIU/L); Cobas: 1.57 mIU/L (95% CI: 1.48–1.75 mIU/L); Atellica: 1.74 mIU/L (95% CI: 1.61–1.83 mIU/L)). Similarly, a trend was seen towards higher median TSH with Cobas than with Alinity among pregnant women (Alinity: 1.90 mIU/L (95% CI: 1.37–2.82 mIU/L); Cobas: 2.33 mIU/L (95% CI: 1.69–3.62 mIU/L)).ConclusionResults of thyroid function tests obtained with different immunoassays were not interchangeable when evaluated among pregnant and non‐pregnant adults. The distinct differences are relevant for clinical decision making and emphasize the necessity of clinical laboratory information when different assays are used for diagnosis and monitoring of patients with thyroid disease.
Influence of novel CYP2C‐haplotype on proton pump inhibitor pharmacokinetics in children
Authors: Kyler Kathryn E. , Gaedigk Andrea , Abdel‐Rahman Susan , Staggs Vincent S. , Pearce Robin E. , Toren Paul , Leeder J. Steven , Shakhnovich Valentina 
Published: April 2024

AbstractIn this brief report, we provide an analysis of the influence of a novel CYP2C haplotype (CYP2C:TG) on proton pump inhibitor (PPI) pharmacokinetics (PK) in children. The CYP2C:TG haplotype has been proposed to be associated with increased CYP2C19 activity. We sought to determine if this CYP2C:TG haplotype resulted in similar alterations in metabolism for proton pump inhibitors, which are primarily metabolized by CYP2C19. In a cohort of 41 children aged 6–21 participating in a PPI pharmacokinetic study, effects of the CYP2C:TG allele were assessed by fitting two linear regression models for each of the six PK outcomes assessed, the second of which accounted for the presence of the CYP2C:TG allele. The difference in R2 values between the two models was computed to quantify the variability in the outcome that could be accounted for by the CYP2C:TG allele after adjustment for the CYP2C19 genotype. We found the CYP2C:TG haplotype to have no measurable additive impact on CYP2C19‐mediated metabolism of PPIs in vivo in older children and adolescents. The findings of this study do not support the clinical utility of routine testing for the CYP2C:TG haplotype to guide PPI dose adjustments in children.
Is vision necessary for the timely acquisition of language‐specific patterns in co‐speech gesture and their lack in silent gesture?
Authors: Özçalışkan Şeyda , Lucero Ché , Goldin‐Meadow Susan 
Published: April 2024

AbstractBlind adults display language‐specificity in their packaging and ordering of events in speech. These differences affect the representation of events in co‐speech gesture–gesturing with speech–but not in silent gesture–gesturing without speech. Here we examine when in development blind children begin to show adult‐like patterns in co‐speech and silent gesture. We studied speech and gestures produced by 30 blind and 30 sighted children learning Turkish, equally divided into 3 age groups: 5–6, 7–8, 9–10 years. The children were asked to describe three‐dimensional spatial event scenes (e.g., running out of a house) first with speech, and then without speech using only their hands. We focused on physical motion events, which, in blind adults, elicit cross‐linguistic differences in speech and co‐speech gesture, but cross‐linguistic similarities in silent gesture. Our results showed an effect of language on gesture when it was accompanied by speech (co‐speech gesture), but not when it was used without speech (silent gesture) across both blind and sighted learners. The language‐specific co‐speech gesture pattern for both packaging and ordering semantic elements was present at the earliest ages we tested the blind and sighted children. The silent gesture pattern appeared later for blind children than sighted children for both packaging and ordering. Our findings highlight gesture as a robust and integral aspect of the language acquisition process at the early ages and provide insight into when language does and does not have an effect on gesture, even in blind children who lack visual access to gesture.Research Highlights
Gestures, when produced with speech (i.e., co‐speech gesture), follow language‐specific patterns in event representation in both blind and sighted children.
Gestures, when produced without speech (i.e., silent gesture), do not follow the language‐specific patterns in event representation in both blind and sighted children.
Language‐specific patterns in speech and co‐speech gestures are observable at the same time in blind and sighted children.
The cross‐linguistic similarities in silent gestures begin slightly later in blind children than in sighted children.
Baby Observational Selective Control AppRaisal (BabyOSCAR): Construct validity and test performance
Authors: Barbosa Vanessa Maziero , Peyton Colleen , Sukal‐Moulton Theresa 
Published: April 2024

AbstractAimTo investigate the construct validity of the Baby Observational Selective Control AppRaisal (BabyOSCAR), an assessment of independent joint motion in infants with cerebral palsy (CP).MethodBabyOSCAR was scored for 75 infants (45 with CP and 30 without CP). Rasch analysis was used in combination with classical test theory to assess areas of strength or improvement. Overall fit and precision, unidimensionality, local independence, reliability indices, Wright's child‐item map, and differential item functioning were examined as part of Rasch analysis to investigate the item properties, internal construct validity, and reliability of BabyOSCAR. Cronbach's α was used to evaluate items’ internal consistency.ResultsAnalysis demonstrated good fit to the Rasch model, with only one erratic item. Unidimensionality results suggest two dimensions, split between arm and leg items. Item calibration reliability was between 0.84 and 0.86, with three distinct item difficulty levels. Infant measure reliability was between 0.82 and 0.91, separating infants into three ability levels. Together, the two subscales covered the full range of skills, with redundancy mostly between the same motion on both sides of the body. Cronbach's α was between 0.90 and 0.95.InterpretationBabyOSCAR's construct validity was supported. Arm and leg subscales can be translated to a logit scale.What this paper adds

Baby Observational Selective Control AppRaisal (BabyOSCAR) has excellent construct validity with good overall fit and precision.
Individual BabyOSCAR items contribute and work well together, forming an interval‐level assessment.
BabyOSCAR has two separate subscales, arms and legs, that complement each other.
BabyOSCAR's items represent a continuum of skills with three distinct difficulty levels.
BabyOSCAR's continuum of skills reliably separates infants into three ability levels.
Evaluation of left ventricular function in patients with mitral annular disjunction using speckle tracking echocardiography
Authors: Özyıldırım Serhan , Guven Baris , Yumuk Mehmet Tugay , Barman Hasan Ali , Dogan Omer , Topel Cagdas , Atici Adem , Donmez Ayça , Kucukoglu Mehmet Serdar , Dogan Sait Mesut 
Published: April 2024

AbstractBackgroundMitral annular disjunction (MAD) is a structural abnormality characterized by the systolic detachment of the posterior mitral annulus and the ventricular myocardium. It is usually observed coexistent with mitral valve prolapse (MVP) and associated with a mechanical dysfunction despite preserved electrical isolation function of the mitral annulus. This study aimed to evaluate left ventricular (LV) function using speckle tracking echocardiography in MVP patients with MAD.MethodsThis study was designed as a prospective, single‐center study including 103 patients with MVP and 40 age‐ and sex‐matched control subjects. Transthoracic echocardiography and cardiac magnetic resonance imaging were performed to assess LV function and MAD presence.ResultsMAD (+) MVP (n = 34), MAD (−) MVP (n = 69), and control (n = 40) groups were enrolled in the study. Among the MVP patients, 34 (33%) had MAD. T‐negativity in the inferior leads on electrocardiography was more frequent in the MAD (+) group than in the MAD (−) patients (4.3% vs. 20.6%, p = .014). Mitral regurgitation degree, Pickelhaube sign (17.6% vs. 1.4%, p = .005), and late gadolinium enhancement frequency (35.3% vs. 10.6%, p = .002) were significantly higher in MAD (+) patients. MAD (+) patients had significantly impaired global longitudinal strain (−23.1 ± 2.1 vs. −23.5 ± 2.3, p < .001), basal longitudinal strain (BLS) (−19.6 ± 1.5 vs. −20.5 ± 1.9, p < .001), Mid‐Ventricular Longitudinal Strain (−22.2 ± 1.7 vs. −23.2 ± 2.2, p < .001) and LA strain (−24.5 ± 3.9 vs. −27.2 ± 3.6, p < .001) when compared to MAD (−) MVP patients, despite similar LV ejection fraction. All these values of MVP patients were also significantly lower than the control group. The mean MAD distance was 7.8 ± 3.2 mm in MAD (+) patients. Patients with two or more symptoms were higher in the MAD (+) group than in the MAD (−) group (4.3% vs. 44.1%, p < .001).ConclusionThis study demonstrated a significant decrease in longitudinal strain in MVP patients with MAD, indicating myocardial dysfunction. These findings suggest that MAD may contribute to LV dysfunction and highlight the importance of early detection in younger patients. Further research is needed to explore the functional implications and long‐term outcomes of MAD.
Therapeutic textiles: A promising approach for human skin dysbiosis?
Authors: de Oliveira Cláudia Suellen Ferro , Tavaria Freni Kekhasharú 
Published: April 2024

AbstractThe close interaction between skin and clothing has become an attractive cornerstone for the development of therapeutic textiles able to alleviate skin disorders, namely those correlated to microbiota dysregulation. Skin microbiota imbalance is known in several skin diseases, including atopic dermatitis (AD), psoriasis, seborrheic dermatitis, rosacea, acne and hidradenitis suppurative (HS). Such microbiota dysregulation is usually correlated with inflammation, discomfort and pruritus. Although conventional treatments, that is, the administration of steroids and antibiotics, have shown some efficacy in treating and alleviating these symptoms, there are still disadvantages that need to be overcome. These include their long‐term usage with side effects negatively impacting resident microbiota members, antibiotic resistance and the elevated rate of recurrence. Remarkably, therapeutic textiles as a non‐pharmacological measure have emerged as a promising strategy to treat, alleviate the symptoms and control the severity of many skin diseases. This systematic review showcases for the first time the effects of therapeutic textiles on patients with skin dysbiosis, focusing on efficacy, safety, adverse effects and antimicrobial, antioxidant and anti‐inflammatory properties. The main inclusion criteria were clinical trials performed in patients with skin dysbiosis who received treatment involving the use of therapeutic textiles. Although there are promising outcomes regarding clinical parameters, safety and adverse effects, there is still a lack of information about the impact of therapeutic textiles on the skin microbiota of such patients. Intensive investigation and corroboration with clinical trials are needed to strengthen, define and drive the real benefit and the ideal biomedical application of therapeutic textiles.
Oral health in individuals with bleeding disorders: A systematic review and meta‐analysis
Authors: Bhadauria Upendra Singh , Purohit Bharathi , Nilima Nilima , Priya Harsh 
Published: April 2024

AbstractBackgroundIndividuals with bleeding disorders have been reported to have a number of oral health issues due to varying conditions. A comprehensive evaluation of the different oral health conditions has not been carried out in the past. This systematic review and meta‐analysis was carried out to collate and critically analyse existing research, and provide a comprehensive overview of the current state of knowledge on oral health.MethodsA comprehensive search was conducted in electronic databases, including PubMed, Scopus and Embase, in October 2023. No restriction on time frame or language was applied. The risk of bias for cross‐sectional studies was assessed using the Agency for Healthcare Research and Quality (AHRQ) tool, and case control studies were assessed using the New Castle Ottawa Scale (NOS).ResultsTwenty‐two articles were included in the final analysis with a total sample size of 2422 subjects. Of the 22 articles assessed, nine quantitative assessments were included in the Meta analysis. Pooled data analysis was carried out. A total of 13 studies reported medium risk whereas the remaining nine studies showed low risk of bias. The weighted mean DMFT scores in individuals with bleeding disorders were found to be 2.43 [0.62. 4.24], mean dmft was 2.79 [1.05, 4.53] and mean OHI‐S was reported to be 1.79 [1.00, 2.57], respectively.ConclusionThe findings emphasize that these individuals have fair oral hygiene and lower dmft/DMFT scores. Oral bleeding emerged as an important oral health component to be cautiously dealt with particularly during the stages of exfoliation/shedding.
RETRACTED: Effect of platelet‐rich plasma combined with negative pressure wound therapy in treating patients with chronic wounds: A meta‐analysis
Authors: Chen Hao , Xu Tong‐Jie , Yu Hao , Zhu Jun‐Long , Liu Yong , Yang Lu‐Pin 
Published: April 2024

AbstractA meta‐analysis was conducted to comprehensively explore the effects of platelet‐rich plasma (PRP) combined with negative pressure wound therapy (NPWT) in treating patients with chronic wounds. Computer searches were conducted, from database infection to November 2023, in EMBASE, Google Scholar, Cochrane Library, PubMed, Wanfang and China National Knowledge Infrastructure databases for randomized controlled trials (RCTs) on the use of PRP combined with NPWT technology for treating chronic wounds. Two researchers independently screened the literature, extracted data and conducted quality assessments according to the inclusion and exclusion criteria. Stata 17.0 software was employed for data analysis. Overall, 18 RCTs involving 1294 patients with chronic wounds were included. The analysis revealed that, compared with NPWT alone, the use of PRP combined with NPWT technology significantly improved the healing rate (odds ratios [OR] = 1.92, 95% confidence intervals [CIs]: 1.43–2.58, p < 0.001) and total effective rate (OR = 1.31, 95% CI: 1.23–1.39, p < 0.001), and also significantly shortened the healing time of the wound (standardized mean difference = −2.01, 95% CI: −2.58 to −1.45, p < 0.001). This study indicates that the treatment of chronic wounds with PRP combined with NPWT technology can significantly enhance clinical repair effectiveness and accelerate wound healing, with a high healing rate, and is worth further promotion and practice.
Czech Republic butterfly barcoding reveals that distribution of genetic lineages depends on species traits
Authors: Sucháčková Bartoňová Alena , Škopek Patrik , Konvička Martin , Beneš Jiří , Spitzer Lukáš , Sbaraglia Claudio , Vrabec Vladimír , Papp Marešová Jana , Konvičková Hana , Faltýnek Fric Zdeněk 
Published: April 2024

AbstractAimThe distribution of within‐species lineages has been affected by Quaternary climate changes, and population differentiation has been influenced by species life history traits. We investigated whether the distribution of individual mitochondrial genetic lineages reflects the constituent species' traits. Using the functionally diverse group of butterflies, we examined which lineages are present in Central Europe, an important suture zone.LocationCzech Republic and Western Palearctic.TaxonA total of 140 butterfly species.MethodsWe sequenced DNA barcodes (cytochrome c oxidase 1) (959 sequences) of the entire Czech Republic butterfly fauna and used Barcoding of Life Data System to visualise the species' biogeographic patterns across Europe. We categorised the distribution patterns of lineages inhabiting the Czech Republic, and used multivariate statistics to interpret these categories by the butterflies' habitats, life history traits and threat levels.ResultsOpen habitat dwellers with specialist traits belonged to Eastern, Southern and temperate lineages. Habitat generalists and woodland dwellers belonged to the Western lineage, formed several lineages or displayed low genetic diversity; they often developed on woody plants, were large‐winged and had long flight periods. The most threatened species were the specialists of Southern, Eastern and temperate lineages.Main ConclusionsThe distribution of lineages in Central Europe reflects the history of Quaternary ecosystems: during cold periods of the Pleistocene, the diverse open habitats prevailed and species could expand westwards. Such species also suffer the most under the current anthropogenic habitat alteration. On the other hand, the mobile generalists and woodland dwellers expanded to Central Europe during the Holocene. Our approach of linking the distribution of lineages with species traits can be transferred to other study systems, and we show that DNA barcoding of under‐sampled areas represents a powerful tool for discovering the driving forces of biogeography.
Enhancing low‐temperature long‐time milk pasteurization process with a C4.5 algorithm‐driven AIoT system for real‐time decision‐making
Authors: Hadi Mokh. Sholihul , Sugiono Bhima Satria Rizki , Mizar Muhammad Alfian , Witjoro Agung , Irvan Mhd 
Published: April 2024

AbstractThis research is driven by the imperative to improve the milk pasteurization process's efficiency and safety within the Industry 5.0 framework. It introduces the artificial intelligence of things (AIoT) into the pasteurization system and focuses on deploying the C4.5 algorithm, anticipated to render precise real‐time decisions by analyzing critical temperature and duration data essential for the low‐temperature long‐time (LTLT) pasteurization technique. Test results indicate that the C4.5 model is highly effective, demonstrating nearly perfect metrics with a precision of .78, a recall of .77, and an F1‐score of .73, while achieving an overall algorithm accuracy of .76. In terms of heating control, the double jacket method tested on a 300‐L system proved the control design's capability to quickly reach and maintain desired temperatures, hitting the set‐point within 70 min. However, it was observed that systems with larger capacities took longer to reach the set‐point, suggesting a potential for further optimization. The findings affirm that integrating AIoT into control systems can significantly enhance process efficiency and effectiveness, consistent with the goal of incorporating sophisticated technological solutions in the food sector.Practical ApplicationsThe article “Enhancing Low‐Temperature Long‐Time Milk Pasteurization Process with a C4.5 Algorithm‐Driven AIoT System for Real‐Time Decision‐Making” explores an AIoT system's potential to revolutionize the dairy industry's pasteurization methods. This system promises enhanced efficiency by optimizing energy and time, thereby reducing costs. It ensures superior quality control through continuous monitoring, maintaining nutritional integrity and safety. The high automation level minimizes human error and operational demands, while predictive maintenance forecasts prevent downtime. The system's scalability supports production expansion with minimal resource increase. Moreover, it guarantees product traceability and compliance with food safety regulations. The generated data offers insights for further supply chain optimizations. This integration of AI and IoT thus presents significant advancements in process management, quality assurance, and scalable operations for the dairy industry.
Spatial distribution of tumor‐resident macrophages as predictive biomarkers in endometrial cancer
Authors: Matsukawa Tetsuya , Yoshikawa Nobuhisa , Liu Wenting , Hattori Satomi , Yoshida Kosuke , Yoshihara Masato , Tamauchi Satoshi , Yokoi Akira , Niimi Kaoru , Kajiyama Hiroaki 
Published: April 2024

AbstractBackgroundTo investigate the role of CD47 expression and its relationship with tumor‐resident macrophages, specifically at the tumor margin, in patients with type II endometrial cancer. This study aims to elucidate whether CD47 could serve as a prognostic marker and to understand the dynamics between CD47 and macrophages, which could inform new therapeutic strategies.MethodsA retrospective cohort study was conducted involving 75 patients of type II endometrial. Immunohistochemical analysis was performed to assess CD47 expression and macrophage markers (CD68 and CD163).ResultsThe study found no direct correlation between CD47 expression levels and overall survival (p = 0.32), challenging its role as an independent prognostic marker in type II endometrial cancer. The higher expression of CD47 had significantly less incidence of endometrioid carcinoma G3 (p = 0.047). The negative correlation between CD47 H‐score and the density of CD68‐positive macrophages at tumor margin was statistically significant (p = 0.049). A high density of CD68‐positive macrophages at the tumor margin but a low density of CD163‐positive macrophages at the tumor margin were associated with poorer prognosis (p = 0.036).ConclusionsThe complex interaction between CD47 and macrophages, particularly at the tumor margin, suggests new avenues for targeted therapy in type II endometrial cancer.