bluuebunny/crossref_metadata_2025_split huggingface dataset:
Should in the treatment of osteochondritis dissecans biodegradable or metallic fixation devices be used? A comparative study in goat knees
Authors: Wouters Diederick B. , Bos Rudolf R. M. , van Horn Jim R. , van Luyn Marja J. A. 
Published: May 2007

AbstractMost of the metallic devices have to be removed, treating osteochondritis dissecans lesions. This animal study describes the biological and mechanical behavior of screws and pins, made of commercially available PGA/PLA and PLA96 and metallic screws and pins, used for fragment fixation. A sham operation served as control. A tissue reaction with cavity formation was observed around every PGA/PLA screw, beginning at 12 weeks following insertion, in contrast to once around a PLA96 screw (p< 0.001), once around one of the 16 PGA/PLA pins and never around those, made of PLA96 (no significance). Disintegration of the PGA/PLA devices started 6 weeks following implantation against 34 weeks for the PLA96 implants. The gap between the fragment and the recipient cartilage disappeared only in the sham group. Many fragments of PGA/PLA material were found in the synovia, in contrast with just a few fragments in the PLA96 group, causing a mild cellular reaction. No polymer particles were found in the draining lymph nodes at any interval. In conclusion, the tested biodegradable screws should not be used for fragment fixation in the treatment of osteochondritis dissecans. Either an undesirable tissue reaction can be expected (PGAPLA), or, because of the slow degradation (PLLA), a screw might damage the opposite cartilage during weight bearing. Two biodegradable pins provide a safe rotational stability and should be combined with one metallic screw, providing compression. This screw has to be removed before loading the limb to prevent cartilage wear of the opposite tibia plateau. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2008
Cinnabarinic acid generated from 3‐hydroxyanthranilic acid strongly induces apoptosis in thymocytes through the generation of reactive oxygen species and the induction of caspase
Authors: Hiramatsu Rie , Hara Toshiaki , Akimoto Hidetoshi , Takikawa Osamu , Kawabe Tsutomu , Isobe Ken‐ichi , Nagase Fumihiko 
Published: May 2007

Abstract3‐Hydroxyanthranilic acid (3HAA) is one of the tryptophan metabolites along the kynurenine pathway and induces apoptosis in T cells. We investigated the mechanism of 3HAA‐induced apoptosis in mouse thymocytes. The optimal concentration of 3HAA for apoptosis induction was 300–500 µM. The induction of apoptosis by a suboptimal concentration (100 µM) of 3HAA was enhanced by superoxide dismutase (SOD) as well as MnCl2 and further promoted in the presence of catalase. The 3HAA‐mediated generation of intracellular reactive oxygen species (ROS) was enhanced by SOD or MnCl2 and inhibited by catalase. Corresponding to apoptosis induction, the generation of cinnabarinic acid (CA) through the oxidation of 3HAA was enhanced by SOD or MnCl2 in the presence of catalase. The synthesized CA possessed more than 10 times higher apoptosis‐inducing activity than 3HAA. The intracellular ROS generation was induced by CA within 15 min and decreased to the control levels within 4 h, whereas the 3HAA‐induced ROS generation increased gradually up to 4 h. Corresponding to ROS generation, the mitochondrial membrane potential was downregulated within 15 min and retained by the CA treatment. Apoptosis induction by 3HAA or CA was dependent on caspases, and caspase‐3 was much more strongly activated by CA than 3HAA. In conclusion, the CA generated from 3HAA possesses a strong apoptosis‐inducing activity in thymocytes through ROS generation, the loss of mitochondrial membrane potential, and caspase activation. J. Cell. Biochem. 103: 42–53, 2008. © 2007 Wiley‐Liss, Inc.
A novel semi‐empirical topological descriptor Nt and the application to study on QSPR/QSAR
Authors: Zhou Congyi , Nie Changming , Li Shan , Li Zhonghai 
Published: May 2007

AbstractA novel semi‐empirical topological descriptor Nt was proposed by revising the traditional distance matrix based on the equilibrium electronegativity and the relative bond length. Nt can not only efficiently distinguish structures of organic compounds containing multiple bonds and/or heteroatoms, but also possess good applications of QSPR/QSAR (quantitative structure‐property/activity relationships) to a large diverse set of compounds, which are alkanes, alkenes, alkynes, aldehydes, ketones, thiols, and alkoxy silicon chlorides with all the correlation coefficients of the models over 0.99. The LOO CV (leave‐one‐out cross‐validation) method was used to testify the stability and predictive ability of the models. The validation results verify the good stability and predictive ability of the models employing the cross‐validation parameters: RCV, SEPCV and SCV, which demonstrate the wide potential of the Nt descriptor for applications to QSPR/ QSAR. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
A systematic quantum chemical investigation of the CH bond activation in methane by gas phase vanadium oxide cation VO+
Authors: Pykavy Mikhail , Van Wüllen Christoph 
Published: May 2007

AbstractThe interaction between a methane molecule and the VO+ cation in the gas phase has been investigated by means of single reference density functional (B3LYP) and wave function‐based multireference (MR) correlation calculations. For the latter, an extrapolation technique is used to evaluate correlation energies at the basis set limit. A comprehensive picture for the CH activation features a variety of molecular structures corresponding to both minima and transition states. Possible reaction paths are discussed, also taking into account change of the spin multiplicity. Activation of the methane molecule by VO+ is always an endothermic process. Competing reaction paths might be expected. An evaluation of miscellaneous computational methods is performed using calculated energy differences for various molecular structures. Results obtained from the MR calculations exhibit no systematic convergence with increasing size of the active space used, and for two largest active spaces relative energies still differ by up to 25 kJ/mol. Simple mean difference between the B3LYP results and the best MR values is −50 ± 19 kJ/mol. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Model transition states for methane diazonium ion methylation of guanine runs in oligomeric DNA
Authors: Ekanayake Kaushalya S. , Lebreton Pierre R. 
Published: May 2007

AbstractThe DNA reaction pattern of the methane diazonium ion, which is the reactive intermediate formed from several carcinogenic methylating agents, was examined at N7 and O6 sites in guanine runs occurring in oligonucleotides and model oligonucleotides. Density functional B3LYP/6‐31G*, and SCF 3‐21G and STO‐3G energies of model transition states were calculated in the gas phase and in the CPCM reaction field. For nucleotides containing two, three, and four stacked guanines with counterions in the gas phase, O6 reactivity is greater than N7 reactivity. In the reaction field, N7 reactivity is 9.0 to 9.8 times greater than O6 reactivity. For a double‐stranded oligonucleotide containing two stacked guanines with counterions in the reaction field, the N7 and O6 reactivities of the 3′‐guanine are 3.9 times greater than the corresponding sites in the 5′‐guanine. For double‐stranded oligonucleotides with three or four stacked guanines and counterions, the reactivities of the interior guanines are higher than corresponding reactivities of guanines at the ends. These reaction patterns agree with most of the available experimental data. Activation energy decomposition analysis for gas‐phase reactions in a double‐stranded dinucleotide containing two stacked guanines with counterions indicates that selectivity at O6 is almost entirely due to electrostatic forces. Selectivity at N7 also has a large electrostatic interaction. However, the orbital interaction also contributes significantly to the gas‐phase selectivity, accounting for 32% of the total interaction energy difference between the 3′‐ and 5′‐guanine reactions. In aqueous solution, the relative orbital contribution to N7 selectivity is likely to be larger. © 2007 Wiley Periodicals, Inc. J Comput Chem 2007
Windock: Structure‐based drug discovery on windows‐based PCs
Authors: Hu Zengjian , Southerland William 
Published: May 2007

AbstractIn recent years, virtual database screening using high‐throughput docking (HTD) has emerged as a very important tool and a well‐established method for finding new lead compounds in the drug discovery process. With the advent of powerful personal computers (PCs), it is now plausible to perform HTD investigations on these inexpensive PCs. To make HTD more accessible to a broad community, we present here WinDock, an integrated application designed to help researchers perform structure‐based drug discovery tasks under a uniform, user friendly graphical interface for Windows‐based PCs. WinDock combines existing small molecule searchable three‐dimensional (3D) libraries, homology modeling tools, and ligand‐protein docking programs in a semi‐automatic, interactive manner, which guides the user through the use of each integrated software component. WinDock is coded in C++. © 2007 Wiley Periodicals, Inc. J Comput Chem 28: 2347–2351, 2007
Cytokine profile of human adipose‐derived stem cells: Expression of angiogenic, hematopoietic, and pro‐inflammatory factors
Authors: Kilroy Gail E. , Foster Sandra J. , Wu Xiying , Ruiz Joseph , Sherwood Sonya , Heifetz Aaron , Ludlow John W. , Stricker Dawn M. , Potiny Suma , Green Patrick , Halvorsen Yuan‐Di C. , Cheatham Bentley , Storms Robert W. , Gimble Jeffrey M. 
Published: May 2007

AbstractAdipose tissue serves as a source of adipokines and cytokines with both local and systemic actions in health and disease. In this study, we examine the hypothesis that multipotent human adipose‐derived stem cells (ASCs), capable of differentiating along the adipocyte, chondrocyte, and osteoblast pathways, contribute to adipose tissue‐derived cytokine secretion. Following exposure to basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF), the ASCs significantly increase their secretion of hepatocyte growth factor (HGF), a cytokine implicated in hematopoiesis, vasculogenesis, and mammary epithelial duct formation. Ascorbic acid synergizes with these inductive factors, further increasing HGF levels. Following exposure to lipopolysaccharide, ASCs increase their secretion of both hematopoietic (granulocyte/monocyte, granulocyte, and macrophage colony stimulating factors, interleukin 7) and proinflammatory (interleukins 6, 8, and 11, tumor necrosis factor α) cytokines based on ELISA and RT‐PCR. In co‐cultures established with umbilical cord blood‐derived CD34+ cells, the ASCs support long‐term hematopoiesis in vitro. Furthermore, in short‐term 12‐day co‐cultures, the ASC maintain and expand the numbers of both myeloid and lymphoid progenitors. These observations are consistent with the functionality of the secreted cytokines and confirm recent reports by other laboratories concerning the hematopoietic supportive capability of ASCs. We conclude that the ASCs display cytokine secretory properties similar to those reported for bone marrow‐derived mesenchymal stem cells (MSCs). J. Cell. Physiol. 212:702–709, 2007. © 2007 Wiley‐Liss, Inc.
Regulation of Na,K‐ATPase α1 subunit gene transcription in response to low K+: Role of CRE/ATF‐ and GC box‐binding proteins
Authors: Wang Gang , Kawakami Kiyoshi , Gick Gregory 
Published: May 2007

AbstractNa,K‐ATPase expression is upregulated in mammalian cells as a consequence of persistent inhibition of Na,K‐ATPase enzymatic activity by low external K+. We previously demonstrated that exposure of neonatal rat cardiac myocytes to low K+ increased Na,K‐ATPase α1 subunit mRNA content and promoter activity. In this work, we utilized transient transfection studies with rat Na,K‐ATPase α1 subunit 5′‐flanking region deletion plasmids to identify DNA sequences required for low K+‐mediated stimulation of α1 subunit promoter expression in cardiac myocytes. Maximal low K+‐responsiveness of the α1 promoter was found to be dependent on nucleotides from −102 to −62 and a downstream region from +53 to +261. Further analysis of the upstream low K+‐responsive region using mutant constructs revealed that a CRE/ATF site at −70 to −63 and a GC box motif at −57 to −48 were both required for the effect of low K+ on α1 subunit gene transcription. Electrophoretic mobility shift assays revealed that low K+ increased binding of transcription factors to the GC box and, to a lesser extent, to the CRE/ATF site. Western blot analysis demonstrated that exposure of cardiac myocytes to low K+ resulted in increased nuclear content of Sp1, Sp3 and CREB‐1. Finally, a selective increase in phosphorylation of Sp1 was found in nuclear extracts from low K+‐treated cells. We conclude that low K+‐mediated upregulation of Na,K‐ATPase α1 subunit gene expression in neonatal rat cardiac myocytes is dependent, in part, on CRE/ATF‐ and GC box‐binding transcription factors. J. Cell. Physiol. 213: 167–176, 2007. © 2007 Wiley‐Liss, Inc.
Differential gene expression induction by TRAIL in B chronic lymphocytic leukemia (B‐CLL) cells showing high versus low levels of Zap‐70
Authors: Secchiero Paola , di Iasio Maria Grazia , Gonelli Arianna , Barbarotto Elisa , Melloni Elisabetta , Tiribelli Mario , Chiaruttini Cristina , Zauli Giorgio 
Published: May 2007

AbstractAmong 14 peripheral blood samples obtained from patients affected by B chronic lymphocytic leukemia (B‐CLL) at initial stages (Rai 0–1) of the disease, 6 showed intermediate/high levels of Zap‐70 while 8 displayed low/absent levels of Zap‐70. Although Zap‐70high and Zap‐70low B‐CLL samples displayed similar levels of surface death receptor TRAIL‐R2, recombinant TRAIL induced cytotoxicity only in a subset of Zap‐70low B‐CLL samples while Zap‐70high were completely resistant to TRAIL. The gene expression profiling was next analyzed in all B‐CLL samples treated with either chlorambucil or recombinant TRAIL. While chlorambucil up‐regulated the steady‐state mRNA levels of known p53 target genes, such as PUMA, Fas/CD95 and MDM2 in all B‐CLL samples examined, it significantly down‐regulated survivin in Zap‐70low but not in Zap‐70high. On the other hand, recombinant TRAIL up‐regulated the expression of several cytokines (IL‐1β, IL‐1α, IL‐8), which have been involved in promoting B‐CLL cell survival. In particular, TRAIL selectively up‐regulated IL‐1β in Zap‐70low B‐CLL samples, while it markedly and selectively up‐regulated its own mRNA and that of cyclooxigenase‐2 (COX‐2) in Zap‐70high. Taken together, our findings suggest that a significant expression of Zap‐70 modulate the response of B‐CLL to TRAIL, which might represents an initial step in the pathogenesis of B‐CLL. J. Cell. Physiol. 213: 229–236, 2007. © 2007 Wiley‐Liss, Inc.
PIM‐2 is an independent regulator of chondrocyte survival and autophagy in the epiphyseal growth plate
Authors: Bohensky Jolene , Shapiro Irving M. , Leshinsky Serge , Watanabe Hitoshi , Srinivas Vickram 
Published: May 2007

AbstractThe overall goal of the investigation was to examine the activity and role of the PIM serine/threonine protein kinases in the growth plate. We showed for the first time that PIM‐2 was highly expressed in epiphyseal chondrocytes and that the kinase was required for critical activities linked to cell survival. These activities were independent of those mediated by Akt‐1. It was noted that PIM‐2 protected chondrocytes from rapamycin sensitized (TOR inhibited) cell death. Since inhibition of mTOR caused autophagy, we examined the autophagic response of PIM‐2 silenced cells. We showed that PIM‐2 promoted expression and organization of autophagic proteins LC3, and Beclin‐1 and enhanced lysosomal acidification. At the same time, PIM‐2 modulated the activity of a key regulator of apoptosis, BAD. Since BAD inhibition and Beclin‐1 expression activated autophagy, it is likely that induction of the autophagic pathway would serve to inhibit apoptosis and preserve the life of the terminally differentiated chondrocyte. We conclude that PIM‐2 regulates a new intermediate stage in the differentiation pathway, the induction of autophagy. J. Cell. Physiol. 213: 246–251, 2007. © 2007 Wiley‐Liss, Inc.
Methylation suppresses the proteasome‐inhibitory function of green tea polyphenols
Authors: Landis‐Piwowar Kristin R. , Wan Sheng Biao , Wiegand Richard A. , Kuhn Deborah J. , Chan Tak Hang , Dou Q. Ping 
Published: May 2007

AbstractUnder physiological conditions, biotransformation reactions, such as methylation, can modify green tea polyphenols (GTPs) and therefore limit their in vivo cancer‐preventive activity. Although a recent study suggested that methylated polyphenols are less cancer‐protective, the molecular basis is unknown. We previously reported that ester bond‐containing GTPs, for example (−)‐epigallocatechin‐3‐gallate [(−)‐EGCG] or (−)‐epicatechin‐3‐gallate [(−)‐ECG], potently and specifically inhibit the proteasomal chymotrypsin‐like activity. In this study, we hypothesize that methylated GTPs have decreased proteasome‐inhibitory abilities. To test this hypothesis, methylated (−)‐EGCG and (−)‐ECG analogs that can be found in vivo were synthesized and studied for their structure‐activity relationships (SARs) using a purified 20S proteasome. The addition of a single methyl group on (−)‐EGCG or (−)‐ECG led to decreased proteasome inhibition and, as the number of methyl groups increased, the inhibitory potencies further decreased. These SARs were supported by our findings from in silico docking analysis published recently. Previously, we synthesized a peracetate‐protected (−)‐EGCG molecule, Pro‐EGCG (1), to enhance its cellular permeability and stability, and current HPLC analysis confirms conversion of Pro‐EGCG (1) to (−)‐EGCG in cultured human leukemic Jurkat T cells. Furthermore, in this study, peracetate‐protected forms of methylated GTPs were added in intact Jurkat T cells to observe the intracellular effects of methylation. Peracetate‐protected, monomethylated (−)‐EGCG induced greater cellular proteasome inhibition and apoptosis than did peracetate‐protected, trimethylated (−)‐EGCG, consistent with the potencies of the parent methylated analogs against a purified 20S proteasome. Therefore, methylation on GTPs, under physiological conditions, could decrease their proteasome‐inhibitory activity, contributing to decreased cancer‐preventive effects of tea consumption. J. Cell. Physiol. 213: 252–260, 2007. © 2007 Wiley‐Liss, Inc.
Non‐essential dietary factors: from test tube to lifespan
Authors: Hillyard S Luke , German J Bruce 
Published: May 2007

AbstractThe agriculture and food industries have an opportunity to change the future of food production by providing foods containing non‐essential dietary factors (NEDFs) that improve health beyond a freedom from disease. In order to accomplish this in a safe and time‐effective manner, however, each NEDF needs to be tested on a number of levels before it is incorporated into foods. In vitro assays are useful for discovering potential beneficial NEDFS, but in vivo studies are needed to both confirm positive effects and to detect negative effects. The genetic and metabolic similarities between humans and Caenorhabditis elegans, combined with the ease with which this nematode can be manipulated in the laboratory make it an attractive candidate to fulfill the role of an early stage in vivo model. Measuring the lifespan of C. elegans cultured in the presence of a range of concentrations of a NEDF would be of both mechanistic and strategic value in identifying those NEDFs that warrant further testing. Copyright © 2007 Society of Chemical Industry
Is hepatic transplantation justified for primary liver cancer?
Authors: Fisher Robert A. , Maluf Daniel G. , Wolfe Luke , Williams Bridgette , Cotterell Adrian , Stravitz R. Todd , Heuman Douglas , Posner Marc 
Published: May 2007

AbstractA concise, yet objective overview of Hepatocellular Carcinoma (HCC) treatment in 2006 with an intent to transplant is presented. The most significant variables impacting on the use of hepatic transplantation as therapy for primary liver cancer are developed under the headings of: Staging Criteria; Organ allocation; Transplant dropout minimization therapies; and Effects on the HCC general population. The pertinent medical literature and update of an ongoing intent‐to‐treat HCC with transplant single center randomized control trial are reviewed. J. Surg. Oncol. 2007;95:674–679. © 2007 Wiley‐Liss, Inc.
Measurement of limb volume: Laser scanning versus volume displacement
Authors: McKinnon John Gregory , Wong Vanessa , Temple Walley J. , Galbraith Callum , Ferry Paul , Clynch George S. , Clynch Colin 
Published: May 2007

AbstractBackgroundDetermining the prevalence and treatment success of surgical lymphedema requires accurate and reproducible measurement. A new method of measurement of limb volume is described.MethodsA series of inanimate objects of known and unknown volume was measured using digital laser scanning and water displacement. A similar comparison was made with 10 human volunteers. Digital scanning was evaluated by comparison to the established method of water displacement, then to itself to determine reproducibility of measurement.Results(1) Objects of known volume: Laser scanning accurately measured the calculated volume but water displacement became less accurate as the size of the object increased. (2) Objects of unknown volume: As average volume increased, there was an increasing bias of underestimation of volume by the water displacement method. The coefficient of reproducibility of water displacement was 83.44 ml. In contrast, the reproducibility of the digital scanning method was 19.0 ml. (3) Human data: The mean difference between water displacement volume and laser scanning volume was 151.7 ml (SD ± 189.5). The coefficient of reproducibility of water displacement was 450.8 ml whereas for laser scanning it was 174 ml.ConclusionLaser scanning is an innovative method of measuring tissue volume that combines precision and reproducibility and may have clinical utility for measuring lymphedema. J. Surg. Oncol. 2007;96:381–388. © 2007 Wiley‐Liss, Inc.
Laser crystals and ceramics: recent advances
Authors: Kaminskii A.A. 
Published: May 2007

AbstractAfter a brief examination of known insulating laser crystals and the stimulated emission channels of their generating activator ions, this article reviews recent advances in the development of novel lasing crystals and ceramics, as well as inorganic and organic nonlinear‐laser crystals for χ(3)and cascaded χ(3)↔ χ(2)frequency converters. Several new modern attractive technologies in the physics and techniques of crystalline lasers are also discussed.
On the Quasiasymptotic of Schwartz Distributions
Authors: Pilipovic Stevan 
Published: May 2007

AbstractThe quasiasymptotic of distributions with the supports in (0, ∞) was studied by the Soviet mathematicians Vladimirov, Drožinov and Zavialov in several papers. Some essential questions on the quasiasymptotic behaviour of Schwartz distributions defined on R are posed and answered in this paper.
On Interpolation Spaces with the Gelfand‐Phillips Property
Authors: Mastylo Mieczyslaw 
Published: May 2007

AbstractIn this paper we study interpolation spaces generated by some interpolation functors. We show that under some conditions for Banach couples X and Y the spaces dual to the orbits of elements are Gelfand—Philips spaces. Consequently, the ideal of nuclear operators from X to Y contains a copy of l1. We give also an interpolation theorem for limited operators.