bluuebunny/crossref_metadata_2025_split huggingface dataset:
New material of the zosterophyllopsid Gosslingia from the Lower Devonian of Guizhou, southwestern China
Authors: Wang Yiling , Liu Lu , Huang Pu , Qin Min , Xue Jinzhuang 
Published: April 2024

AbstractThe zosterophyllopsids had a widespread distribution and constituted a dominant component in many plant assemblages during the Early Devonian. Although a large number of zosterophyllopsids have been documented, knowledge about the paleogeographic distribution of different genera/species remains to be expanded by further fossil evidence. In this article, new material assigned to the genus Gosslingia Heard, 1927 and designated as Gosslingia cf. G. breconensis Heard, 1927 is described from a new locality of the Lower Devonian of Guizhou Province, China. The Guizhou material shows main axes that are pseudomonopodially branched, pseudomonopodial lateral branching systems, subaxillary tubercle branches, circinate apices, elliptical xylem strand, exarch maturation of xylem, and G-type tracheids, and exhibits considerable similarities with the type and only species of Gosslingia, Gosslingia breconensis. Our finding represents the first report of Gosslingia in the South China Block and the most convincing occurrence of this genus outside of Wales, UK. Gosslingia adds to the diversity of genera shared among the Early Devonian floras of South China, western Europe, and North America, along with Distichophytum Mägdefrau, Estinnophyton Fairon-Demaret, Zosterophyllum Penhallow, and others, and indicates that the dispersal of early vascular plants among different paleocontinents was more common than previously appreciated.
The Extrajudicial Voice
Authors: Mallory Conall , Tyrrell Hélène 
Published: April 2024

AbstractJudges communicate outside of the courtroom on a regular basis. They give speeches at universities and to societies; appear before select committees; write for a range of publications; and engage in both media and outreach activities. Existing literature has charted the value and perils of such extrajudicial communication. This paper contributes an explanation of what motivates judges towards such communication, and what shapes their discourse. The work draws on 13 semi-structured interviews with senior serving and recently retired judges, along with an extensive range of examples of judicial speech beyond the bench. It argues that extrajudicial communication is shaped by a shared conception amongst the judicial community of what is appropriate. This conception of propriety is principally motivated by a communal pursuit of sustaining public confidence in the judicial office. The conception also informs the limits of appropriate discourse and establishes the ramifications for breach.
APPROXIMATION OF IRRATIONAL NUMBERS BY PAIRS OF INTEGERS FROM A LARGE SET
Authors: DUBICKAS ARTŪRAS 
Published: April 2024

AbstractWe show that there is a set
$S \subseteq {\mathbb N}$
with lower density arbitrarily close to
$1$
such that, for each sufficiently large real number
$\alpha $
, the inequality
$|m\alpha -n| \geq 1$
holds for every pair
$(m,n) \in S^2$
. On the other hand, if
$S \subseteq {\mathbb N}$
has density
$1$
, then, for each irrational
$\alpha>0$
and any positive
$\varepsilon $
, there exist
$m,n \in S$
for which
$|m\alpha -n|<\varepsilon $
.
Corals Reveal Interdecadal Variation of Tropical Cyclones Modulated by Pacific Decadal Oscillation
Authors: Han Yansong , Jiang Wei , Jiang Leilei , Yong Yangyang , Yang Kai , Gu Tingwu , Guo Ning , Yu Kefu 
Published: April 2024

AbstractTropical cyclones (TCs) lead to huge economic losses and casualties in coastal regions, but multi‐decadal variation of TC remains poorly understood due to the limited length of observational records and low‐resolution reconstructions. Porites corals provide continuous and monthly resolved records that allow for the determination of weather‐timescale (weekly to monthly) extreme events, including TC, to overcome the above limitations. Here we synthesize five coral Δδ18O records from the South China Sea (SCS) and develop a coral TC frequency index, in good agreement with post‐1980 instrumental records and sediment‐based TC reconstructions over the past century. Our results indicate that TC frequency in the SCS exhibits pronounced decadal variations, with active TCs corresponding to the negative phase of the Pacific Decadal Oscillation. Our findings here provide novel evidence for past TC changes in the SCS and a constraint for predicting future TC changes.
Orthogonal analysis of mitochondrial function in Parkinson’s disease patients
Authors: Barnhoorn Sander , Milanese Chiara , Li Tracy , Dons Lieke , Ghazvini Mehrnaz , Sette Martina , Farina Stefania , Sproviero Daisy , Payan-Gomez Cesar , Mastroberardino Pier G. 
Published: April 2024

AbstractThe etiopathology of Parkinson’s disease has been associated with mitochondrial defects at genetic, laboratory, epidemiological, and clinical levels. These converging lines of evidence suggest that mitochondrial defects are systemic and causative factors in the pathophysiology of PD, rather than being mere correlates. Understanding mitochondrial biology in PD at a granular level is therefore crucial from both basic science and translational perspectives. In a recent study, we investigated mitochondrial alterations in fibroblasts obtained from PD patients assessing mitochondrial function in relation to clinical measures. Our findings demonstrated that the magnitude of mitochondrial alterations parallels disease severity. In this study, we extend these investigations to blood cells and dopamine neurons derived from induced pluripotent stem cells reprogrammed from PD patients. To overcome the inherent metabolic heterogeneity of blood cells, we focused our analyses on metabolically homogeneous, accessible, and expandable erythroblasts. Our results confirm the presence of mitochondrial anomalies in erythroblasts and induced dopamine neurons. Consistent with our previous findings in fibroblasts, we observed that mitochondrial alterations are reversible, as evidenced by enhanced mitochondrial respiration when PD erythroblasts were cultured in a galactose medium that restricts glycolysis. This observation indicates that suppression of mitochondrial respiration may constitute a protective, adaptive response in PD pathogenesis. Notably, this effect was not observed in induced dopamine neurons, suggesting their distinct bioenergetic behavior. In summary, we provide additional evidence for the involvement of mitochondria in the disease process by demonstrating mitochondrial abnormalities in additional cell types relevant to PD. These findings contribute to our understanding of PD pathophysiology and may have implications for the development of novel biomarkers and therapeutic strategies.
Eph-ephrin signaling couples endothelial cell sorting and arterial specification
Authors: Stewen Jonas , Kruse Kai , Godoi-Filip Anca T. , Zenia  , Jeong Hyun-Woo , Adams Susanne , Berkenfeld Frank , Stehling Martin , Red-Horse Kristy , Adams Ralf H. , Pitulescu Mara E. 
Published: April 2024

AbstractCell segregation allows the compartmentalization of cells with similar fates during morphogenesis, which can be enhanced by cell fate plasticity in response to local molecular and biomechanical cues. Endothelial tip cells in the growing retina, which lead vessel sprouts, give rise to arterial endothelial cells and thereby mediate arterial growth. Here, we have combined cell type-specific and inducible mouse genetics, flow experiments in vitro, single-cell RNA sequencing and biochemistry to show that the balance between ephrin-B2 and its receptor EphB4 is critical for arterial specification, cell sorting and arteriovenous patterning. At the molecular level, elevated ephrin-B2 function after loss of EphB4 enhances signaling responses by the Notch pathway, VEGF and the transcription factor Dach1, which is influenced by endothelial shear stress. Our findings reveal how Eph-ephrin interactions integrate cell segregation and arteriovenous specification in the vasculature, which has potential relevance for human vascular malformations caused byEPHB4mutations.
Shaping active matter from crystalline solids to active turbulence
Authors: Yang Qianhong , Jiang Maoqiang , Picano Francesco , Zhu Lailai 
Published: April 2024

AbstractActive matter drives its constituent agents to move autonomously by harnessing free energy, leading to diverse emergent states with relevance to both biological processes and inanimate functionalities. Achieving maximum reconfigurability of active materials with minimal control remains a desirable yet challenging goal. Here, we employ large-scale, agent-resolved simulations to demonstrate that modulating the activity of a wet phoretic medium alone can govern its solid-liquid-gas phase transitions and, subsequently, laminar-turbulent transitions in fluid phases, thereby shaping its emergent pattern. These two progressively emerging transitions, hitherto unreported, bring us closer to perceiving the parallels between active matter and traditional matter. Our work reproduces and reconciles seemingly conflicting experimental observations on chemically active systems, presenting a unified landscape of phoretic collective dynamics. These findings enhance the understanding of long-range, many-body interactions among phoretic agents, offer new insights into their non-equilibrium collective behaviors, and provide potential guidelines for designing reconfigurable materials.
Mesenchymal glioma stem cells trigger vasectasia—distinct neovascularization process stimulated by extracellular vesicles carrying EGFR
Authors: Spinelli Cristiana , Adnani Lata , Meehan Brian , Montermini Laura , Huang Sidong , Kim Minjun , Nishimura Tamiko , Croul Sidney E. , Nakano Ichiro , Riazalhosseini Yasser , Rak Janusz 
Published: April 2024

AbstractTargeting neovascularization in glioblastoma (GBM) is hampered by poor understanding of the underlying mechanisms and unclear linkages to tumour molecular landscapes. Here we report that different molecular subtypes of human glioma stem cells (GSC) trigger distinct endothelial responses involving either angiogenic or circumferential vascular growth (vasectasia). The latter process is selectively triggered by mesenchymal (but not proneural) GSCs and is mediated by a subset of extracellular vesicles (EVs) able to transfer EGFR/EGFRvIII transcript to endothelial cells. Inhibition of the expression and phosphorylation of EGFR in endothelial cells, either pharmacologically (Dacomitinib) or genetically (gene editing), abolishes their EV responses in vitro and disrupts vasectasia in vivo. Therapeutic inhibition of EGFR markedly extends anticancer effects of VEGF blockade in mice, coupled with abrogation of vasectasia and prolonged survival. Thus, vasectasia driven by intercellular transfer of oncogenic EGFR may represent a new therapeutic target in a subset of GBMs.
Age-progressive interplay of HSP-proteostasis, ECM-cell junctions and biomechanics ensures C. elegans astroglial architecture
Authors: Coraggio Francesca , Bhushan Mahak , Roumeliotis Spyridon , Caroti Francesca , Bevilacqua Carlo , Prevedel Robert , Rapti Georgia 
Published: April 2024

AbstractTissue integrity is sensitive to temperature, tension, age, and is sustained throughout life by adaptive cell-autonomous or extrinsic mechanisms. Safeguarding the remarkably-complex architectures of neurons and glia ensures age-dependent integrity of functional circuits. Here, we report mechanisms sustaining the integrity of C. elegans CEPsh astrocyte-like glia. We combine large-scale genetics with manipulation of genes, cells, and their environment, quantitative imaging of cellular/ subcellular features, tissue material properties and extracellular matrix (ECM). We identify mutants with age-progressive, environment-dependent defects in glial architecture, consequent disruption of neuronal architecture, and abnormal aging. Functional loss of epithelial Hsp70/Hsc70-cochaperone BAG2 causes ECM disruption, altered tissue biomechanics, and hypersensitivity of glia to environmental temperature and mechanics. Glial-cell junctions ensure epithelia-ECM-CEPsh glia association. Modifying glial junctions or ECM mechanics safeguards glial integrity against disrupted BAG2-proteostasis. Overall, we present a finely-regulated interplay of proteostasis-ECM and cell junctions with conserved components that ensures age-progressive robustness of glial architecture.
Global spatiotemporal synchronizing structures of spontaneous neural activities in different cell types
Authors: Shi Liang , Fu Xiaoxi , Gui Shen , Wan Tong , Zhuo Junjie , Lu Jinling , Li Pengcheng 
Published: April 2024

AbstractIncreasing evidence has revealed the large-scale nonstationary synchronizations as traveling waves in spontaneous neural activity. However, the interplay of various cell types in fine-tuning these spatiotemporal patters remains unclear. Here, we performed comprehensive exploration of spatiotemporal synchronizing structures across different cell types, states (awake, anesthesia, motion) and developmental axis in male mice. We found traveling waves in glutamatergic neurons exhibited greater variety than those in GABAergic neurons. Moreover, the synchronizing structures of GABAergic neurons converged toward those of glutamatergic neurons during development, but the evolution of waves exhibited varying timelines for different sub-type interneurons. Functional connectivity arises from both standing and traveling waves, and negative connections can be elucidated by the spatial propagation of waves. In addition, some traveling waves were correlated with the spatial distribution of gene expression. Our findings offer further insights into the neural underpinnings of traveling waves, functional connectivity, and resting-state networks, with cell-type specificity and developmental perspectives.
Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage
Authors: Zhang Xiaomei , Ge Lihao , Jin Guanghui , Liu Yasong , Yu Qingfen , Chen Weizhao , Chen Liang , Dong Tao , Miyagishima Kiyoharu J. , Shen Juan , Yang Jinghong , Lv Guo , Xu Yan , Yang Qing , Ye Linsen , Yi Shuhong , Li Hua , Zhang Qi , Chen Guihua , Liu Wei , Yang Yang , Li Wei , Ou Jingxing 
Published: April 2024

AbstractCold-induced injuries severely limit opportunities and outcomes of hypothermic therapies and organ preservation, calling for better understanding of cold adaptation. Here, by surveying cold-altered chromatin accessibility and integrated CUT&Tag/RNA-seq analyses in human stem cells, we reveal forkhead box O1 (FOXO1) as a key transcription factor for autonomous cold adaptation. Accordingly, we find a nonconventional, temperature-sensitive FOXO1 transport mechanism involving the nuclear pore complex protein RANBP2, SUMO-modification of transporter proteins Importin-7 and Exportin-1, and a SUMO-interacting motif on FOXO1. Our conclusions are supported by cold survival experiments with human cell models and zebrafish larvae. Promoting FOXO1 nuclear entry by the Exportin-1 inhibitor KPT-330 enhances cold tolerance in pre-diabetic obese mice, and greatly prolongs the shelf-life of human and mouse pancreatic tissues and islets. Transplantation of mouse islets cold-stored for 14 days reestablishes normoglycemia in diabetic mice. Our findings uncover a regulatory network and potential therapeutic targets to boost spontaneous cold adaptation.
Dopamine control of social novelty preference is constrained by an interpeduncular-tegmentum circuit
Authors: Molas Susanna , Freels Timothy G. , Zhao-Shea Rubing , Lee Timothy , Gimenez-Gomez Pablo , Barbini Melanie , Martin Gilles E. , Tapper Andrew R. 
Published: April 2024

AbstractAnimals are inherently motivated to explore social novelty cues over familiar ones, resulting in a novelty preference (NP), although the behavioral and circuit bases underlying NP are unclear. Combining calcium and neurotransmitter sensors with fiber photometry and optogenetics in mice, we find that mesolimbic dopamine (DA) neurotransmission is strongly and predominantly activated by social novelty controlling bout length of interaction during NP, a response significantly reduced by familiarity. In contrast, interpeduncular nucleus (IPN) GABAergic neurons that project to the lateral dorsal tegmentum (LDTg) were inhibited by social novelty but activated during terminations with familiar social stimuli. Inhibition of this pathway during NP increased interaction and bout length with familiar social stimuli, while activation reduced interaction and bout length with novel social stimuli via decreasing DA neurotransmission. These data indicate interest towards novel social stimuli is encoded by mesolimbic DA which is dynamically regulated by an IPN→LDTg circuit to control NP.
A distal enhancer guides the negative selection of toxic glycoalkaloids during tomato domestication
Authors: Bai Feng , Shu Peng , Deng Heng , Wu Yi , Chen Yao , Wu Mengbo , Ma Tao , Zhang Yang , Pirrello Julien , Li Zhengguo , Hong Yiguo , Bouzayen Mondher , Liu Mingchun 
Published: April 2024

AbstractSteroidal glycoalkaloids (SGAs) are major plant defense metabolites against pests, while they are considered poisonous in food. The genetic basis that guides negative selection of SGAs production during tomato domestication remains poorly understood. Here, we identify a distal enhancer, GAME Enhancer 1 (GE1), as the key regulator of SGAs metabolism in tomato. GE1 recruits MYC2-GAME9 transcriptional complex to regulate the expression of GAME cluster genes via the formation of chromatin loops located in the neighboring DNA region. A naturally occurring GE176 allelic variant is found to be more active in stimulating GAME expression. We show that the weaker GE1 allele has been the main driver for selecting reduced SGAs levels during tomato domestication. Unravelling the “TFs-Enhancer-Promoter” regulatory mechanism operating in SGAs metabolism opens unprecedented prospects for SGAs manipulation in Solanaceae via precision breeding strategies.
Up-bottom assessments of nutrient supply and gaseous pollutant from Chinese wheat straw field management
Authors: Li Dongxue , Gu Jun , Chen Xiaoqin , Wang Yiliu , Lu Dianjun , Yokamo Solomon , Wang Huoyan , Hou Peng 
Published: April 2024

AbstractTo achieve resource efficiency, and carbon neutrality, it is vital to evaluate nutrient supply and gaseous pollutant emissions associated with field management of bio-straw resources. Previous straw yield estimates have typically relied on a constant grain-to-straw yield ratio without accounting for grain yield levels in a given region. Addressing this high-resolution data gap, our study introduces a novel empirical model for quantifying grain-to-straw yield, which has been used to gauge wheat straw field management practices at the city level during 2011–2015. Utilizing both statistical review and GIS-based methods, average nitrogen (N), phosphorus (P), and potassium (K) supplies from straw field management stood at 1510, 1229, and 61700 tons, respectively. Average emissions of PM2.5, SO2, NOx, NH3, CH4, and CO2 due to straw burning were 367, 41, 160, 18, 165, and 70,644 tons, respectively. We also reported uncertainty from Monte Carlo model as the 5th-95th percentiles of estimated nutrient supply and gaseous pollutant. These insights will provide foundational support for the sustainable and environmentally friendly management of wheat straw in China.
Spatiotemporal dynamics of cholera epidemics in Ethiopia: 2015–2021
Authors: Moore Sandra , Worku Demlie Yeshambel , Muluneh Dereje , Dunoyer Jessica , Hussen Mukemil , Wossen Mesfin , Edosa Moti , Sudre Bertrand 
Published: April 2024

AbstractSince the onset of the seventh cholera pandemic, Ethiopia has been affected by recurrent epidemics. However, the epidemiology of cholera in this country remains poorly understood. This study aimed to describe cholera outbreak characteristics in Ethiopia from 2015 to 2021. During this period, Ethiopia experienced four epidemic waves. The first wave involved nationwide outbreaks during the second half of 2016 followed by outbreaks predominantly affecting Somali Region in 2017. The second wave primarily affected Tigray and Afar Regions. During the third wave, multiple smaller-scale outbreaks occurred during 2019. The fourth wave was limited to Bale Zone (Oromia Region) in 2021. Overall, a north to south shift was observed over the course of the study period. Major cholera transmission factors included limited access to safe water and sanitation facilities. Severe weather events (drought and flooding) appear to aggravate cholera diffusion. Cholera transmission between Ethiopia and nearby countries (Kenya and Somalia), likely plays a major role in regional cholera dynamics. Overall, this study provides the first understanding of recent spatiotemporal cholera dynamics in Ethiopia to inform cholera control and elimination strategies.
Comparison of analysis methods to classify cholera hotspots in Ethiopia from 2015 to 2021
Authors: Demlie Yeshambel Worku , Moore Sandra , Dunoyer Jessica , Muluneh Dereje , Hussen Mukemil , Wossen Mesfin , Edosa Moti , Sudre Bertrand 
Published: April 2024

AbstractCholera continues to represent a major public health concern in Ethiopia. The country has developed a Multi-sectoral National Cholera Elimination Plan in 2022, which targets prevention and control interventions in cholera hotspots. Multiple methods to classify cholera hotspots have been used in several countries. Since 2014, a classification method developed by United Nations Children's Fund has been applied to guide water, sanitation and hygiene interventions throughout Sub-Saharan Africa based on three outbreak parameters: frequency, duration and standardized attack rate. In 2019, the Global Task Force on Cholera Control (GTFCC) proposed a method based on two parameters: average annual cholera incidence and persistence. In 2023, an updated GTFCC method for multisectoral interventions considers three epidemiological indicators (cumulative incidence, cumulative mortality and persistence,) and a cholera-case confirmation indicator. The current study aimed to classify cholera hotspots in Ethiopia at the woreda level (equivalent to district level) applying the three methods and comparing the results to optimize the hotspot targeting strategy. From 2015 to 2021, cholera hotspots were located along major routes between Addis Ababa and woredas adjacent to the Kenya and Somalia borders, throughout Tigray Region, around Lake Tana, and in Afar Region. The multi-method comparison enables decision makers to prioritize interventions according to a sub-classification of the highest-priority areas.