bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ovarian cancer exosome-mediated evasion of the anti-angiogenic effect of bevacizumab
Authors: Chen Zhuoye , Wang Conghui , Xu Mingxia , Zhu Tingjia , Wang Yuehan , Cheng Xiaodong , Wang Xinyu 
Published: April 2024

Abstract

Objective
Is there a way for ovarian cancer to escape the antiangiogenic effects of bevacizumab
Methods
Bevacizumab treatment of different types of tumor cells altered the content of VEGF-A both inside and outside the cells.HUVEC endothelial cells were treated with conditioned supernatant and subjected to angiogenesis, proliferation and migration phenotype assays.The exosome release inhibitor GW4869 was used to treat the supernatant, and then the endothelial cell phenotype experiment was performed to verify the role of exosomes in the supernatant. After disrupting the exosome membrane structure, the content of VEGF-A in the supernatant was detected.The exosomes in the supernatant were extracted by the kit, and the VEGF-A content in the exosomes was detected. Database, PCR and WB were used to verify the differential expression of molecules related to cargo sorting such as CD82 in cells treated with bevacizumab. Immunofluorescence was used to verify the co-localization of CD82 and VEGF-A.
Results
VEGF-A content in and outside ovarian cancer cells increased after tumor cells were treated with bevacizumab. Ovarian cancer cell-derived conditioned supernatant can promote angiogenesis, proliferation and migration phenotype of HUVEC, and these effects may be achieved by exosomes. Ovarian cancer may capture VEGF-A by exosomes mediated by CD82, transmit VEGF-A to downstream endothelial cells and promote their angiogenesis, migration and proliferation to achieve the evading effect of bevacizumab.
Conclusion
CD82 may mediate the capture of VEGF-A by ovarian cancer exosomes to escape the anti-angiogenic effect of bevacizumab.
Comparison of proportions and prognostic impact of pathological complete response between evaluations of representative specimen and total specimen in primary breast cancer after neoadjuvant chemoradiotherapy: An ancillary study of JCOG0306
Authors: Shien Tadahiko , Tsuda Hitoshi , Sasaki Keita , Mizusawa Junki , Akiyama Futoshi , Kurosumi Masafumi , Sawaki Masataka , Tamura Nobuko , Tanaka Kiyo , Kogawa Takahiro , Takahashi Mina , Hayashi Naoki , Mukai Hirofumi , Masuda Norikazu , Hara Fumikata , Iwata Hiroji 
Published: April 2024

Abstract
Background
In JCOG0306 trial, a phase II study to examine the efficacy of neoadjuvant chemotherapy followed by radiation therapy (NAC-RT) to primary breast cancer, pathological complete response (pCR) was evaluated from specimens of the representative cross section including the tumor center that had been accurately marked [representative specimen (RS) method]. In this ancillary study we examined if the RS method was comparable to the conventional total specimen (TS) method, which is widely employed in Japan, to identify the pCR group showing excellent prognosis.
Methods
We obtained long-term follow-up data of 103 patients enrolled in JCOG0306 trial. As histological therapeutic effect, pCR (ypT0 and ypT0/is) and quasi pCR [QpCR, ypT0/is plus Grade 2b (only a few remaining invasive cancer cells)] were evaluated with RS and TS methods. Concordance of pCR between these two methods and associations of the pCR with prognosis were examined.
Results
ypT0, ypT0/is, and QpCR were observed in 28 (27.2%), 39 (37.9%) and 45 (43.7%) patients with RS method, whereas these were 20 (19.4%), 25 (24.3%) and 40 (38.9%) with TS method, respectively. Between RS and TS methods, concordance proportions of ypT0 and ypTis were 92.2% and 86.4%, respectively. Risk of recurrence of ypT0/is group was lower than that of non-ypT0/is group (HR 0.408, 95% CI [0.175—0.946], P = 0.037) and risk of death of ypT0/is group was lower than that of non-ypT0/is group (HR 0.251, 95% CI [0.073—0.857], P = 0.027). The ypT0 and ypT0/is groups with RS method showed excellent prognosis similarly with those with TS method, and RS method was able to differentiate the OS and RFS between pCR and non-pCR than TS method significantly even if pCR was classified ypT0 or ypT0/is. With TS method, QpCR criteria stratified patients into the better and worse prognosis groupsmore clearly than pCR criteria of ypT0 or ypT0/is.
Conclusions
RS method was comparable to TS method for the evaluation of pCR in the patients who received NAC-RT to primary breast cancer provided the tumor center was accurately marked. As pCR criteria with RS method, ypT0/is appeared more appropriate than ypT0.
Variational Onsager Neural Networks based Fair proof-of- reputation consensus for blockchain with Transaction Prioritization for Smart Cities
Authors: Singh Chandra Prakash , Agarwal Rohita , Umrao Lokendra Singh 
Published: April 2024

Abstract
Smart cities are next frontier of technology in today's technology-driven world, striving to improve the quality of people's lives. Numerous research projects concentrate on future smart cities, taking comprehensive method to smart city growth, achieving an overall smart city vision. The Variational Onsager Neural Networks based Fair proof-of-reputation consensus for block chain with Transaction Prioritization for Smart Cities (VONN-FPORC-TP-SC) is proposed for transaction prioritization in smart cities. Block chain, as a decentralised immutable ledger, has potential to boost smart city growth by ensuring transparency, data safety, dependability, efficacy, interoperability, privacy, making it promising match for smart cities. Such instance, if existing fee-depend or first-come-first-served processing is applied, emergency events delayed owing to competition, risking people's lives. Here use Variational Onsager Neural Networks (VONN) approach to accomplish efficient leader election and build a Weighted Byzantine Fault Tolerance consensus algorithm mechanism in this consensus protocol. A peer-prediction based verification technique is also presented to verify that followers' assessments of the leaders' created blocks are honest. Also, because leader will assure transaction prioritisation though creating blocks, leader rotation, and correct election method become critical for transaction prioritisation process to taken place honestly, quickly on FPoR: fair proof-of-reputation consensus for block chain. The proposed VONN-FPORC-TP-SC method is implemented on MATLAB R2019b. Then performance of proposed method is analysed with other existing techniques. The proposed method attains 28.86%, 24.47% and 31.79% higher accuracy, 18.25%, 32.27% and 26.89% higher efficiency, and 23.21%, 17.36% and 32.35% higher Robustness comparing with the existing methods such as a ML-improved block chain consensus with transaction prioritization for smart cities (ML-BC-TP-SC), block chain and smart contracts to secure property transactions in smart cities (BCS-TP-SC), presented an Edge TC-a PBFT block chain-depend ETC system for smart cities (PBFT-ETC-TP-SC) respectively.
Heavy Mineral and Clay Mineralogy of Recent Fluvial Sediments Along Brahmaputra River of Dibrugarh District, Assam
Authors: Chakraborty Abhijit 
Published: April 2024

Abstract
This study area delves into the geological saga of the Brahmaputra River in Dibrugarh District, Assam, exploring its profound impact on regional evolution amidst extensive layers of contemporary alluvial sediments, including Older Alluvium, High-Level Terraces, and New Alluvium, visible in natural cut bank formations along the river's course. The recent fluvial sedimentary deposits were meticulously surveyed and sampled at ten distinct sites from Tingkhan – Nagakheliya Gaon, Oakland Ghat, Madhupur Ghat, and Dolphin View Point, Chabua, evenly distributed from north-north-east (NNE) to south-west-west (SWW) along the river. The primary objective of this study is to conduct a thorough analysis of heavy minerals percentages through microscopic slide analysis, aiming to assess the maturity of river sediments utilizing the Zircon-Tourmaline-Rutile (ZTR) maturity index and to elucidate clay mineralogy employing X-ray diffraction techniques of recent fluvial sediments. The findings of these analyses provide valuable insights like, heavy minerals derive from the sediments diverse NNE and SWW sources, with ultra-stable and unstable heavy minerals assemblages suggesting sediments source from high-grade metamorphic and acidic igneous rock regions. The ZTR maturity index of sediments increases in the direction from NNE to SWW. Also rounded Zircon-Tourmaline-Rutile grains imply reworked sediment contribution, while subrounded to sub-angular grains hint at potential first-cycle origin. X-ray diffraction analyses highlight peaks of clay matrix (Kaolinite, Chlorite), and the abundance of non-clay matrix (Garnet, Siderite, Pyrite specially Quartz). This sedimentary record resonates with a captivating amalgamation of influences from the northeastern Himalayas and Naga Hills, coupled with dendritic sediment depositions.
Dissecting the Role of NETosis-Related Biomarkers in Sepsis: An Integrated Multi-Dataset Analysis for Diagnostic and Prognostic Applications
Authors: 方 爱莉 
Published: April 2024

Abstract
1.Abstract:
Sepsis, a systemic and life-threatening response to infection, presents complex challenges in clinical management and prognosis due to its intricate pathophysiology. The formation of Neutrophil Extracellular Traps (NETs) through a process known as NETosis has been identified as a significant contributor to the development of sepsis. This study aimed to dissect the roles of NETosis-related genes, particularly Myeloperoxidase (MPO) and Proteinase 3 (PRTN3), in sepsis progression. By integrating and analyzing multiple Gene Expression Omnibus (GEO) datasets, we conducted a comprehensive gene expression profiling that revealed consistent downregulation of MPO and PRTN3, among others, in sepsis patients. Through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, we characterized the biological functions and pathways associated with these genes, emphasizing their relevance to immune responses in sepsis. A prediction model utilizing these biomarkers was constructed using a Random Forest classifier, which demonstrated robust predictive capability, as reflected by an AUROC of 0.77 for training and 0.68 for validation datasets. Survival analysis further underscored the prognostic value of demographic factors, particularly gender and age. The model highlighted gender-specific survival rates and revealed a significant decline in survival probability in patients over 40 years of age. These findings illuminate the diagnostic and prognostic potential of MPO and PRTN3 in sepsis, offering novel insights into the molecular dynamics of the disease and suggesting a direction for future therapeutic strategies. The study's integrated approach and novel findings advocate for personalized management of sepsis, tailoring interventions to individual patient profiles to improve outcomes.
Marine derivative CHNQD-00603 reverses bone marrow mesenchymal stem cells senescence by enhancing autophagy through the AKT/ERK/mTOR signaling pathway
Authors: Yang Xiaoxia , ren Wenhao , peng Baoying , li Shaoming , Zheng Jingjing , sun Kai , zhi Keqian , Gao Ling 
Published: April 2024

Abstract
Objective
Maxillofacial bone defect caused by the tumor and periodontal disease in the elderly will affect implant restoration. Bone marrow mesenchymal stem cells (BMSCs), as seed cells for bone regeneration, play an important role in the treatment of bone defects. The objective of this study was to investigate the effect and mechanism of marine derivative CHNQD-00603 on senescence BMSCs.
Materials and Methods
Biological function of BMSCs was determined by flow cytometry, alizarin red and oil-red O. Transmission electron microscopy Western blot, qRT-PCR, and reactive oxygen species detection were used to evaluate the effects of CHNQD-00603 on autophagosomes, autophagy-related molecules, senescence-related indicators, and ROS in aging BMSCs. The mechanism of CHNQD-00603 inhibiting BMSCs aging was detected by Western blot and qRT-PCR.
Results
In this study, CHNQD-00603 increased the level of autophagy, and decreased the level of ROS in senescence BMSCs. In addition, CHNQD-OO603 decreased AKT/ERK phosphorylation and increased mTOR phosphorylation. The agonists of AKT and ERK can increase the mRNA expression of age-related genes p16 and p21.
Conclusions
Our findings revealed that CHNQD-OO603 inhibits BMSCs senescence via the AKT/ERK/mTOR signaling pathway. This provides a potential idea for the treatment of insufficient jaw volume in the elderly.
Electrochemical Performance of NiAl Activated Cathode for the Green Hydrogen Production
Authors: Latreche Sofiane , Boutarek-Zaourar Naïma , Bencherifa Ismail , Messaoud Faouzi , Trari Mohamed 
Published: April 2024

Abstract
This work is devoted to the elaboration of Ni68%Al32% wt. (NiAl) intermetallic compound highly electro-active and cost-effective electrode materials using an induction furnace utilized as cathodes in alkaline water reduction into hydrogen. The XRD analysis showed the formation of the NiAl phase with an average crystallite size of 44.13 nm. The SEM/EDS results confirm the XRD results with an average grain size of 50 µm. The chemical activation of Aluminum from NiAl was carried out on NaOH (25%) solution at 353 K for 72 h. The Optical Profilometry and UV-visible spectrophotometry confirmed the formation of Ni-Raney-type electrodes with high specific surface area. The activated NiAl electrodes resulted in remarkable efficiency for the hydrogen evolution reaction (HER), 13 times higher than the non-activated electrode. The comparison with literature data reveals that the efficiency of the NiAl cathode has increased by 120% compared to pure Ni.
Revision and validation of the Health and Safety Executive Management Standards Indicator Tool among health care workers
Authors: Zeinolabedini Masoume , Motlagh Mohammad Esmaeil , Heidarnia Alireza , Shakerinejad Ghodratollah , Zeidi Isa Mohammadi 
Published: April 2024

Abstract
Background: Workplace stress is a serious workplace hazard, especially for high-stress occupations such as healthcare workers. Identifying valid and reliable tools to measure conditions related to workplace stress is essential for targeted interventions. The aim of this study was to determine the psychometric properties of the revised version of the Health & Safety Executive Indicator Tool (HSE).
Methods: An item pool was generated based on the HSE Indicator Tool and the findings of a qualitative study. After face and content validity, the psychometric properties of the instrument were examined. Cronbach's alpha, McDonald's omega and intra-class correlation coefficient were calculated to assess reliability.
Results: A total of 220 health care workers from 80 primary health care centers in 5 cities in Iran completed the Health & Safety Executive-Developed Occupational Stress Indicator Tool (HSE-DOSIT). In exploratory factor analysis, 35 items were loaded, which indicated a 9-factor solution for the questionnaire. Six factors were the same as the original UK version (Superior support, Demands, Job control, Social support, Changes, and Relationships) and three new factors were added (Non-job factors, Cognitive structure and Individual context). Cronbach's alpha (0.949), MacDonald Omega (from 0.739 to 0.894), and intra-class correlation coefficient (0.92) were acceptable
Conclusions: Overall, the results show that the HSE-DOSIT is reliable and valid. In other words, HSE-DOSIT can be useful for identifying organizational, social and individual factors related to workplace stress and implementing targeted interventions. However, further evaluation of HSE-DOSIT is recommended.
Follow-up of health education based on WeChat platform on anxiety, depression and quality of life in patients with pulmonary nodules: a single-center study
Authors: Huang Jiang-Shan , Zhang Zhen-yang , Lin Yu-Kang , Lin Wen-wei , Zhu Jia-fu , Lin Jiang-bo 
Published: April 2024

Abstract
Objective: The influence of health education based on WeChat platform on anxiety, depression and quality of life (QoL) of patients with indeterminate pulmonary nodules (IPNs).
Method: IPNs from January 2020 to June 2022 were selected for follow-up. Self-questionnaire was used to subjectively evaluate patients' mental state, ASA and SDS questionnaires were used to objectively evaluate anxiety and depression, Fear of Progression Questionnaire-Short Form was used to evaluate patients' fear of disease, and SF-36 was used to evaluate patients' QoL.
Results: After 6 months of follow-up, the WeChat group felt significantly less mental stress and sleep disturbance, fewer CT scans and multiple doctor visits than the traditional group; The anxiety, depression and fear of disease in the WeChat group was significantly less than that of the traditional group. The HRQoL of the two groups at 6 months of follow-up, the HRQoL of the WeChat group was lower than that of the traditional group.
Conclusion: WeChat-based health education can effectively reduce anxiety and depression, improve disease fear, and improve HRQoL for patients with IPN; at the same time, it can reduce unnecessary radiation times.
Transformed DETR: Leveraging Dense Prior and Focal Attention for Enhanced Visual Recognition
Authors: Nasaoui Hakim , Bellamine Insaf , Silkan Hassan 
Published: April 2024

Abstract
Object detection is a pivotal task in computer vision, with applications in numerous areas including surveillance, autonomous driving, and image analysis. Traditional methods for object detection required handcrafted feature extraction and the use of classifiers to label objects within images. With the advent of deep learning, these processes have been significantly automated, leading to advancements in both accuracy and efficiency of object detection systems. In this work, we introduce a novel architecture that incorporates a Dense Prior module and Focal Self-attention mechanism into the DEtection TRansformer (DETR) framework. This Transformed DETR model is designed to direct greater focus to regions of interest in images, thereby improving detection performance. We demonstrate the effectiveness of our approach on the COCO dataset, achieving a Mean Average Precision (mAP) of 53.8%, which indicates a considerable improvement over existing methods. Our architecture captures and focus on the key regions within an image, resulting in a more powerful object detection model.
Discovery of Conformationally Constrained Dihydro Benzo-Indole Derivatives as Pan-Metallo-β-Lactamase Inhibitors to Tackle Multidrug-Resistant Bacterial Infections
Authors: Chaudhari Vinod , Dhiman Pulkit , Das Satyajeet , Pathania Vikas , Rawat Suraj , Nandanwar Hemraj , Thakur Krishan 
Published: April 2024

Abstract
The discovery of pan-metallo-β-lactamase (pan-MBL) inhibitors is crucial in the fight against bacterial infection following the emergence and rapid spread of New Delhi metallo-β-lactamase-1 (NDM-1), as well as clinically relevant Verona integrin-encoded metallo-β-lactamase (VIM), and Imipenemase (IMP). The situation is alarming as there are insufficient antibiotics in the pipeline to combat critical multi-drug resistant infections. Here, we report the discovery of novel Dihydro Benzo-Indole (dBI) derivatives as a new class of potent pan-metallo-β-lactamase inhibitors (MBLIs) by applying scaffold hopping, conformation constrained, and substituent-decorating strategies. Among them, compound 17u exhibited best inhibitory activity against pan-MBL with acceptable physicochemical and ADME properties. 17u exhibited remarkable enhancement of carbapenems' effectiveness against a range of MBL-producing clinical strains. This efficacy extended to in vivo settings when combined with the imipenem antibiotic, significantly reducing bacterial load in a thigh infection model. Consequently, it qualifies as a prime candidate for further development as a MBLI.
Anxiety Disorder Amongst Clinical Medical Students at the University of Nigeria Teaching Hospital, Enugu: a Comparative Study
Authors: PRECIOUS ANASIUDU EBUBE , CHINEDU ANAKWUE RAPHAEL , CHUKWUEBUKA ANASONYE JOHNBOSCO , PAUL ANANWUNA NNAGOZIE 
Published: April 2024

Abstract
Anxiety disorders are combinations of emotional disorders that have profound effects on the mental status of individuals. One of the most common presentations is a panic attack, in which students experience attacks that are especially close to tests and examinations due to the cumbersome academic workload.
This paper reviews the effect of anxiety disorders among medical students at the University of Nigeria Enugu Campus. An online questionnaire was distributed to the 3rd, 4th, and 5th MBBS classes in an attempt to determine the prevalence and knowledge of anxiety in these classes, and any known coping mechanisms and to perform a comparative study on socio-demographic, academic, and personal factors.
The results revealed a preponderance in females and fear of failure and a high dependence on receiving emotional support from family, praying and meditating as coping mechanisms.
Hopefully, this study will inform academic authorities on the need to adjust syllables due to increased workload as a contributing factor of anxiety disorder and school authorities on the need for a closed student-lecturer interaction.
Glymphatic dysfunction and choroid plexus volume increase in older adults with poor sleep quality
Authors: Kikuta Junko 
Published: April 2024

Abstract
Objective
This study aimed to explore alterations in diffusion tensor image analysis along the perivascular space (DTI-ALPS) method and choroid plexus volume (CPV), which could be biomarkers of the glymphatic system in older adults with poor sleep quality (PSQ).
Methods
Fifty-two Japanese older adults with Pittsburgh Sleep Quality Index (PSQI) scores > 5 (22 men and 30 women; mean age ± SD = 73.10 ± 5.67 years) and 52 healthy controls (HCs; PSQI score ≤ 5) were included. Diffusion-weighted imaging (DWI) and 3D T1-weighted images were obtained using 3T magnetic resonance imaging. The ALPS index was calculated using preprocessed diffusion-weighted imaging (DWI), and the CPV was calculated using FreeSurfer 6.0. The mean ALPS index was subsequently compared between the PSQ group and HCs by using a general linear model (GLM) adjusted for covariates, including age, sex, years of education, intracranial volume, systolic blood pressure, diastolic blood pressure, hemoglobin A1c, and white matter lesion volume (WMLV). The CPV was also compared between the two groups by using the GLM, adjusting for the same covariates mentioned above. Next, we conducted a partial correlation analysis between the ALPS index and the CPV, Montreal Cognitive Assessment (MoCA), and PSQI scores, adjusting for all the abovementioned covariates.
Results
Compared with HCs, patients in the PSQ group had a significantly lower mean ALPS (p = 0.04, Cohen’s d = − 0.28) and a greater CPV (p = 0.11, Cohen’s d = 0.24). In the PSQ group, the mean ALPS score was significantly negatively correlated with the CPV (r = − 0.35, false discovery rate [FDR]-corrected p = 0.03) but was significantly positively correlated with the MoCA score (r = 0.35, FDR-corrected p = 0.03).
Conclusion
Older adults with PSQ exhibited a decrease in the glymphatic system and an increase in CPV.