bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of vaccination on the association of COVID-19 with cardiovascular diseases: An OpenSAFELY cohort study
Authors: Cezard Genevieve I. , Denholm Rachel E. , Knight Rochelle , Wei Yinghui , Teece Lucy , Toms Renin , Forbes Harriet J. , Walker Alex J. , Fisher Louis , Massey Jon , Hopcroft Lisa E. M. , Horne Elsie M. F. , Taylor Kurt , Palmer Tom , Arab Marwa Al , Cuitun Coronado Jose Ignacio , Ip Samantha H. Y. , Davy Simon , Dillingham Iain , Bacon Sebastian , Mehrkar Amir , Morton Caroline E. , Greaves Felix , Hyams Catherine , Davey Smith George , Macleod John , Chaturvedi Nishi , Goldacre Ben , Whiteley William N. , Wood Angela M. , Sterne Jonathan A. C. , Walker Venexia ,   On behalf of the Longitudinal Health and Wellbeing and Data and Connectivity UK COVID-19 National Core Studies, CONVALESCENCE study and the OpenSAFELY collaborative
Published: March 2024

AbstractInfection with SARS-CoV-2 is associated with an increased risk of arterial and venous thrombotic events, but the implications of vaccination for this increased risk are uncertain. With the approval of NHS England, we quantified associations between COVID-19 diagnosis and cardiovascular diseases in different vaccination and variant eras using linked electronic health records for ~40% of the English population. We defined a ‘pre-vaccination’ cohort (18,210,937 people) in the wild-type/Alpha variant eras (January 2020-June 2021), and ‘vaccinated’ and ‘unvaccinated’ cohorts (13,572,399 and 3,161,485 people respectively) in the Delta variant era (June-December 2021). We showed that the incidence of each arterial thrombotic, venous thrombotic and other cardiovascular outcomes was substantially elevated during weeks 1-4 after COVID-19, compared with before or without COVID-19, but less markedly elevated in time periods beyond week 4. Hazard ratios were higher after hospitalised than non-hospitalised COVID-19 and higher in the pre-vaccination and unvaccinated cohorts than the vaccinated cohort. COVID-19 vaccination reduces the risk of cardiovascular events after COVID-19 infection. People who had COVID-19 before or without being vaccinated are at higher risk of cardiovascular events for at least two years.
Identification of gene regulatory networks affected across drug-resistant epilepsies
Authors: François Liesbeth , Romagnolo Alessia , Luinenburg Mark J. , Anink Jasper J. , Godard Patrice , Rajman Marek , van Eyll Jonathan , Mühlebner Angelika , Skelton Andrew , Mills James D. , Dedeurwaerdere Stefanie , Aronica Eleonora 
Published: March 2024

AbstractEpilepsy is a chronic and heterogenous disease characterized by recurrent unprovoked seizures, that are commonly resistant to antiseizure medications. This study applies a transcriptome network-based approach across epilepsies aiming to improve understanding of molecular disease pathobiology, recognize affected biological mechanisms and apply causal reasoning to identify therapeutic hypotheses. This study included the most common drug-resistant epilepsies (DREs), such as temporal lobe epilepsy with hippocampal sclerosis (TLE-HS), and mTOR pathway-related malformations of cortical development (mTORopathies). This systematic comparison characterized the global molecular signature of epilepsies, elucidating the key underlying mechanisms of disease pathology including neurotransmission and synaptic plasticity, brain extracellular matrix and energy metabolism. In addition, specific dysregulations in neuroinflammation and oligodendrocyte function were observed in TLE-HS and mTORopathies, respectively. The aforementioned mechanisms are proposed as molecular hallmarks of DRE with the identified upstream regulators offering opportunities for drug-target discovery and development.
Simulating photosynthetic energy transport on a photonic network
Authors: Tang Hao , Shang Xiao-Wen , Shi Zi-Yu , He Tian-Shen , Feng Zhen , Wang Tian-Yu , Shi Ruoxi , Wang Hui-Ming , Tan Xi , Xu Xiao-Yun , Wang Yao , Gao Jun , Kim M. S. , Jin Xian-Min 
Published: March 2024

AbstractQuantum effects in photosynthetic energy transport in nature, especially for the typical Fenna-Matthews-Olson (FMO) complexes, are extensively studied in quantum biology. Such energy transport processes can be investigated as open quantum systems that blend the quantum coherence and environmental noise, and have been experimentally simulated on a few quantum devices. However, the existing experiments always lack a solid quantum simulation for the FMO energy transport due to their constraints to map a variety of issues in actual FMO complexes that have rich biological meanings. Here we successfully map the full coupling profile of the seven-site FMO structure by comprehensive characterisation and precise control of the evanescent coupling of the three-dimensional waveguide array. By applying a stochastic dynamical modulation on each waveguide, we introduce the base site energy and the dephasing term in coloured noise to faithfully simulate the power spectral density of the FMO complexes. We show our photonic model well interprets the phenomena including reorganisation energy, vibrational assistance, exciton transfer and energy localisation. We further experimentally demonstrate the existence of an optimal transport efficiency at certain dephasing strength, providing a window to closely investigate environment-assisted quantum transport.
Co-densification of rice straw and cow dung in different food-to-microorganism ratios for biogas production
Authors: Singh Prakash , Dogra Pallavi , TG Induchoodan , Kalamdhad Ajay S. 
Published: March 2024

AbstractAgricultural residues such as rice straw (RS) are desirable raw materials for biogas generation. However, the recalcitrant nature of RS hinders biogas production, and its low bulk density increases storage space requirements, transportation needs, and overall costs. These challenges could be resolved by pretreatment and pelletization. In this study, various thermal pretreatments were performed, and the best conditions (temperature and time) were identified. Also, rice straw and cow dung pellets (RCP) at different food-to-microorganism (F/M) ratios (0.5–2.5) were prepared. Parameters such as bulk density, moisture absorption, and drop shatter tests were conducted to evaluate the physical properties. Finally, the biochemical methane potential (BMP) study of the best RCP with varying total solids (TS: 4–12%) content was investigated. The results indicate that hot air oven pretreatment (for 60 min at 120 °C) resulted in maximum solubilization. In physical characteristics, RCP with an F/M ratio of 2.5 pellets was ideal. The bulk density of RCP 2.5 was found to be around 25 times that of the raw. Also, the TS 8% yielded maximum biomethane (279 mL/g-VSconsumed) as compared to other TS contents. Overall, this study will propel the growth of bioenergy while simultaneously tackling the pressing issues related to RS management.
Seepage evolution characteristics and water inrush mechanism in collapse column under mining influence
Authors: Yongjiang Wu , Zhengzheng Cao , Zhenhua Li , Feng Du , Wenqiang Wang , Minglei Zhai , Zijie Hong , Yi Xue 
Published: March 2024

AbstractTo obtain the seepage evolution rule and water inrush mechanism of the collapse column, a multi-field coupled mechanical model for water inrush disasters caused by the collapse column is established in this paper, on the basis of the specific engineering conditions of the 1908 working face in the Qianjin coal mine. The mechanical model is composed of internal column elements within the collapse column and surrounding rock masses. The research focuses on the seepage evolution rule in the roof collapse column under different mining conditions and investigates the permeation instability mechanism of collapse column based on the transition of flow state. The research results indicate that the seepage pathway evolves continuously, ultimately forming a channel for water inrush, as the working face advances towards the collapse column. Besides, the water inflow increases rapidly when the working face advances 100 m, then gradually stabilizes, indicating that the seepage channel entry of the collapse column is in a stable stage. Meanwhile, mass loss in the collapse column gradually moves upward. the collapse column remains stable as a whole in the initial stage of water flow, with a small permeability, exhibiting linear flow. As time steps increases, particle loss in collapse column gradually extends to the upper part, forming a stable seepage channel. The flow velocity shows fluctuations with a slow declining trend over time.
Enhancing co-seismic landslide susceptibility, building exposure, and risk analysis through machine learning
Authors: Pyakurel Ajaya , K.C. Diwakar , Dahal Bhim Kumar 
Published: March 2024

AbstractLandslides are devastating natural disasters that generally occur on fragile slopes. Landslides are influenced by many factors, such as geology, topography, natural drainage, land cover, rainfall and earthquakes, although the underlying mechanism is too complex and very difficult to explain in detail. In this study, the susceptibility mapping of co-seismic landslides is carried out using a machine learning approach, considering six districts covering an area of 12,887 km2 in Nepal. Landslide inventory map is prepared by taking 23,164 post seismic landslide data points that occurred after the 7.8 MW 2015 Gorkha earthquake. Twelve causative factors, including distance from the rupture plane, peak ground acceleration and distance from the fault, are considered input parameters. The overall accuracy of the model is 87.2%, the area under the ROC curve is 0.94, the Kappa coefficient is 0.744 and the RMSE value is 0.358, which indicates that the performance of the model is excellent with the causative factors considered. The susceptibility thus developed shows that Sindhupalchowk district has the largest percentage of area under high and very high susceptibility classes, and the most susceptible local unit in Sindhupalchowk is the Barhabise municipality, with 19.98% and 20.34% of its area under high and very high susceptibility classes, respectively. For the analysis of building exposure to co-seismic landslide susceptibility, a building footprint map is developed and overlaid on the co-seismic landslide susceptibility map. The results show that the Sindhupalchowk and Dhading districts have the largest and smallest number of houses exposed to co-seismic landslide susceptibility. Additionally, when conducting a risk analysis based on susceptibility mapping, as well as considering socio-economic and structural vulnerability in Barhabise municipality, revealed that only 106 (1.1%) of the total 9591 households, were found to be at high risk. As this is the first study of co-seismic landslide risk study carried out in Nepal and covers a regional to the municipal level, this can be a reference for future studies in Nepal and other parts of the world and can be helpful in planning development activities for government bodies.
Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)
Authors: Nwe May Khat , Jangpromma Nisachon , Taemaitree Lapatrada 
Published: March 2024

AbstractLoop-mediated isothermal amplification (LAMP) is a cost-effective and easy-to-perform assay that enables the direct detection of DNA. Its use in point-of-care diagnostic tests is growing, while it has the potential to be used in presumptive on-the-field forensic tests. Samples are often collected from complex matrices that contain high levels of contaminants. Herein, we evaluate the effect of seven common DNA amplification inhibitors on LAMP – bile salts, calcium chloride, hematin, humic acid, immunoglobulin G, tannic acid and urea. We study the effect of each inhibitor individually in real-time detection systems coupled with end-point measurements to delineate their inhibitory effects from the matrix in which they may be found. Our studies show LAMP inhibitors generally delay the onset of amplicon formation and quench fluorescence at similar or higher concentrations compared to PCR, but that end-point measurements of LAMP amplicons are unaffected. This is important as LAMP amplicons can be detected in non-fluorometric ways thus contributing to the assertions that LAMP is more robust to inhibitors than PCR.
Investigations on the effect of natural veined calcite on the mechanical properties of limestone
Authors: Chen Qingzhi , Liu Yuanming , Teng Zhaolei , Ou Xun , Zhang Quan 
Published: March 2024

AbstractThe damage behavior of limestone rock masses containing calcite mineral filling under uniaxial compression experimental conditions is unclear, and the fracture mechanism of the rock masses needs to be further explored. In this study, uniaxial compression tests were conducted on limestone rock specimens containing veined calcite by combining acoustic emission and digital image correlation techniques. The effects of veined calcite on the generation and development of cracks on the surface of the specimens until the formation of macroscopic penetration and the strength properties of the rock mass were analyzed. The results showed that the transversely distributed veined calcite caused significant stress concentrations in the rock specimens. The longitudinally distributed veined calcite caused cracks in the specimens or influenced the expansion path of the longitudinal principal cracks. The final damage pattern of the specimens didn’t differ significantly from that of conventional rock masses due to the presence of veined calcite. The presence of the veined calcite had effect on the uniaxial compressive capacity of the rock, but the load variation process of the specimen with time still conformed to the load variation pattern during the uniaxial compressive test of conventional rocks.
Arthroscopic debridement improves range of motion for heterotopic ossification after total knee replacement: a retrospective cohort study
Authors: Zhang Dong-Liang , Zhang Wei , Ren Yi-Ming , Zhao Wen-Jun , Sun He-Jun , Tian Zheng-Wei , Tian Meng-Qiang 
Published: March 2024

AbstractThe presence of heterotopic ossification (HO) after primary total knee replacement (TKR) is rare and associated with limited mobility and stiffness of the knee. This study aimed to identify if the arthroscopic debridement after TKR could decrease HO and improve the function and range of motion. Thirty HO patients after TKR were retrospectively separated into 2 cohorts. 15 patients of group A accepted the arthroscopic debridement, while 15 patients of group B only had non-operative treatment, mainly including oral nonsteroidal anti-inflammatory drugs (NSAIDs) and rehabilitative treatment. Visual analog scale (VAS) scores, knee society knee scores (KSS), range of motion (knee flexion and knee extension) were obtained before treatment and at 1 month, 3 months, and 6 months after treatment. Radiography of after-treatment was also evaluated to assess the changes in HO. There were 3 males and 27 females with a mean age of 67.4 ± 0.8 years in group A and 68.2 ± 1.3 in group B. The onset time of HO was 3–6 months. The maximum size of the ossification was < 2 cm in 23 knees, 2 cm < heterotopic bone < 5 cm in 6 knees and > 5 cm in 1 knee. The size of HO decreased gradually in all knees by X-ray film at the last follow-up. There were no significant differences in VAS scores after replacement between two groups (p > 0.05). The average range of motion preoperatively in group A was − 15.2–90.6°, which postoperatively increased to − 4.2–110.0°. Meanwhile, the KSS scores and average range of motion of the group A were better than those of the group B at each follow-up time after treatment. Arthroscopic debridement can decrease HO seen from postoperative X-rays, improve the function and range of motion, as well as the pain remission between two groups are comparable. Consequently, arthroscopic resection of HO after TKR is recommended as soon as there is aggravating joint stiffness.
NT-proBNP ratio is a potential predictor for COVID-19 outcomes in adult Chinese patients: a retrospective study
Authors: Li Dan , He Wu , Yu Bo , Wang Dao Wen , Ni Li 
Published: March 2024

AbstractDespite the progressive decline in the virulence of the novel coronavirus, there has been no corresponding reduction in its associated hospital mortality. Our aim was to redefine an accurate predictor of mortality risk in COVID-19 patients, enabling effective management and resource allocation. We conducted a retrospective analysis of 2917 adult Chinese patients diagnosed with COVID-19 who were admitted to our hospital during two waves of epidemics, involving the Beta and Omicron variants. Upon admission, NT-proBNP levels were measured, and we collected demographic, clinical, and laboratory data. We introduced a new concept called the NT-proBNP ratio, which measures the NT-proBNP level relative to age-specific maximum normal values. The primary outcome was all-cause in-hospital mortality. Our analysis revealed a higher in-hospital mortality rate in 2022, as shown by the Kaplan–Meier Survival Curve. To assess the predictive value of the NT-proBNP ratio, we employed the time-dependent receiver operating characteristic (ROC) curve. Notably, the NT-proBNP ratio emerged as the strongest predictor of mortality in adult Chinese hospitalized COVID-19 patients (area under the curve, AUC = 0.826; adjusted hazard ratio [HR], 3.959; 95% confidence interval [CI] 3.001–5.221; P < 0.001). This finding consistently held true for both the 2020 and 2022 subgroups. The NT-proBNP ratio demonstrates potential predictive capability compared to several established risk factors, including NT-proBNP, hsCRP, and neutrophil-to-lymphocyte ratio, when it comes to forecasting in-hospital mortality among adult Chinese patients with COVID-19.Trial registration Clinical Trial Registration: www.clinicaltrials.gov NCT05615792.
Single-cell RNA sequencing reveals critical modulators of extracellular matrix of penile cavernous cells in erectile dysfunction
Authors: Luo Chao , Peng Yaqian , Gu Jiang , Li Tao , Wang Qiang , Qi Xiaolan , Wei Anyang 
Published: March 2024

AbstractErectile dysfunction (ED) is a common and difficult to treat disease, and has a high incidence rate worldwide. As a marker of vascular disease, ED usually occurs in cardiovascular disease, 2–5 years prior to cardiovascular disease events. The extracellular matrix (ECM) network plays a crucial role in maintaining cardiac homeostasis, not only by providing structural support, but also by promoting force transmission, and by transducing key signals to intracardiac cells. However, the relationship between ECM and ED remains unclear. To help fill this gap, we profiled single-cell RNA-seq (scRNA-seq) to obtain transcriptome maps of 82,554 cavernous single cells from ED and non-ED samples. Cellular composition of cavernous tissues was explored by uniform manifold approximation and projection. Pseudo-time cell trajectory combined with gene enrichment analysis were performed to unveil the molecular pathways of cell fate determination. The relationship between cavernous cells and the ECM, and the changes in related genes were elucidated. The CellChat identified ligand-receptor pairs (e.g., PTN-SDC2, PTN-NCL, and MDK-SDC2) among the major cell types in the cavernous tissue microenvironment. Differential analysis revealed that the cell type-specific transcriptomic changes in ED are related to ECM and extracellular structure organization, external encapsulating structure organization, and regulation of vasculature development. Trajectory analysis predicted the underlying target genes to modulate ECM (e.g., COL3A1, MDK, MMP2, and POSTN). Together, this study highlights potential cell–cell interactions and the main regulatory factors of ECM, and reveals that genes may represent potential marker features of ED progression.
Effects of thienopyridine class antiplatelets on bleeding outcomes following robot-assisted radical prostatectomy
Authors: Kubota Masashi , Kawakita Mutsushi , Yoshida Satomi , Kimura Hiroko , Sumiyoshi Takayuki , Yamasaki Toshinari , Okumura Kazuhiro , Yoshimura Koji , Matsui Yoshiyuki , Sugiyama Kyohei , Okuno Hiroshi , Segawa Takehiko , Shimizu Yosuke , Ito Noriyuki , Onishi Hiroyuki , Ishitoya Satoshi , Soda Takeshi , Yoshida Toru , Uemura Yuichi , Iwamura Hiroshi , Okubo Kazutoshi , Suzuki Ryosuke , Fukuzawa Shigeki , Akao Toshiya , Kurahashi Ryoma , Shimatani Kimihiro , Sekine Yuya , Negoro Hiromitsu , Akamatsu Shusuke , Kamoto Toshiyuki , Ogawa Osamu , Kawakami Koji , Kobayashi Takashi , Goto Takayuki 
Published: March 2024

AbstractThis study aimed to assess the effects of thienopyridine-class antiplatelet agents (including ticlopidine, clopidogrel, and prasugrel) on bleeding complications in patients who underwent robot-assisted radical prostatectomy. This cohort study used a database for robot-assisted radical prostatectomy at 23 tertiary centers nationwide between 2011 and 2022. Patients who received thienopyridines (thienopyridine group) were compared with those who received aspirin monotherapy (aspirin group). The primary outcome was the incidence of bleeding complications. High-grade complications were defined as Clavien–Dindo grade III or higher. The risks of these outcomes were evaluated using inverse probability of treatment weighted regression models. The study results demonstrated that thienopyridine therapy was associated with a higher risk of overall bleeding complications (OR: 3.62, 95%CI 1.54–8.49). The increased risks of the thienopyridine group were detected for low-grade bleeding complications (OR: 3.20, 95%CI 1.23–8.30) but not for high-grade bleeding complications (OR: 5.23, 95%CI 0.78–34.9). The increased risk of bleeding complications was not observed when thienopyridine was discontinued (OR: 2.52, 95%CI 0.83–7.70); however, it became apparent when it was continued perioperatively (OR: 4.35, 95%CI 1.14–16.61). In conclusion, thienopyridine increased the incidence of bleeding complications, particularly low-grade bleeding complications, following robot-assisted radical prostatectomy. These bleeding effects emerged when thienopyridine was continued perioperatively.