bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytogenetic studies on human myeloma cell lines
Authors: Jernberg H. , Zech L. , Nilsson K. 
Published: February 2007

AbstractCell lines (U‐266, U‐1957, U‐1996 and U‐2030) established from 4 patients with multiple myeloma (MM) were analyzed cytogenetically. The cell lines represent different stages in B‐cell differentiation as evidenced by ultrastructural and functional characteristics. The karyotypic pattern in 3 newly established myeloma lines was studied after a few months in culture and compared to the old myeloma cell line U‐266, which was examined after 6, 7 and 8 years of continuous cultivation. Frequency of progressive numerical and structural aberrations during long‐term cultivation and their correlation with alterations in growth properties were addressed. We describe the presence of a high frequency of both numerical and structural chromosomal abnormalities in the cells of all 4 myeloma lines studied. Chromosomes often associated with structural abnormalities were 1,3,6,12 and 14. A 14q + marker chromosome was detected in 2 of the 4 cell lines. The breakpoints on the chromosomes participating in structural aberrations in myeloma exhibit some correlation to chromosome sites at or close to locations of mapped oncogenes. No translocations of c‐myc were found. These data were further supported by Southern blot analysis (unpublished data). The extent of numerical, but not structural, aberrations correlates with the differentiation stage of the myeloma lines in that the 2 mature lines U‐266 and U‐1957 were both near‐diploid. Multiple progressive chromosomal changes have emerged in U‐266 during a period of 8 years with development of independence of feeder cells and increased growth rate. However, capacity for production of complete Ig molecules has remained stable.
Transplacental induction of a specific mutation in fetal Ha‐ras and its critical role in post‐natal carcinogenesis
Authors: Yamasaki H. , Hollstein M. , Martel N. , Cabral J. R. P. , Galendo D. , Tomatis L. 
Published: February 2007

AbstractMouse skin tumors were produced after transpiacental initiation [with 7, 12‐dimethylbenz(a)anthracene], only when the skin was treated post‐natally with a tumor‐promoting agent (12‐O‐tetradecanoyl phorbol 13‐acetate). DNA analysis of tumors showed that all carcinomas analyzed contained a specific mutation (A to T trans version) at the 61st codon of c‐Ha‐ras. Fifty per cent of the papillomas analyzed also had this same mutation. The A to T trans version at the 61st codon of Ha ras was heterozygous in all positive papillomas and carcinomas No such mutation was found when benzo(a) pyrene was used as an initiating agent. These results indicate that fetal c‐Ha‐ras can be trans placentally activated through a specific point mutation by a carcinogen, but a cell harboring such a mutation may remain dormant until it encounters a tumor promoting stimulus.
Enhanced metastases of a mouse carcinoma after in vitro treatment with murine interferon gamma
Authors: Ramani Pijamila , Balkwill Frances R. 
Published: February 2007

AbstractWe have studied the influence of inter ferons (IFNs) on the metastatic potential of mouse colon adenocarcinoma, COLON 26, cells. Pre‐treatment of the cells in vitro for 24 hr with recombinant murine IFN‐γ (rMuIFN‐γ) significantly in‐creased the number of lung tumour nodules when cells were injected i.v. into immunocompetent BALB/c mice and BALB/ c nude mice. However when MulFN‐γ‐pre‐treated cells were injected into beige (NK‐deficient) nude mice or anti‐asialoGM I (as GMI)‐serum‐treated BALB/c mice (NK‐depleted) no enhancement of metastatic potential was seen. Pre‐treatment of COLON 26 cells with recombinant human IFN‐α A/D (Bgl I), an IFN with equal activity on human and mouse cells, did net significantly enhance their subsequent metastases in immunocompetent or immunodeficient mice. In fact, there was a small but significant decrease in the number of tumour nodules in the lungs of beige nude and asGM I ‐treated mice. The e effects of rMuIFN‐γ on COLON 26 cells did not appear to be related to an alteration in MHC expression. COLON 26 eel Is constitutively express H‐2D and H‐2K antigens and both IFNs had equal enhancing (approx. 2‐fold) activity on the expression of these antigens at the doses used in this experiment (103 U/ml). We conclude that pre‐treatment with rMuIFN‐γ renders COLON 26 cells resistant to in vivo NK‐cell lysis via a mechanism that does not involve changes in MHC expression.
TPA induction of Epstein‐Barr virus early antigens in Raji cells is blocked by selective protein kinase‐C inhibitors
Authors: Lazdins Janis , Zompetta Claudia , Grimaldi Settimio , Barile Giuseppe , Venanzoni Marco , Frati Luigi , Faggioni Alberto 
Published: February 2007

AbstractWe have shown that modulation of intracellular calcium in EBV latently infected cells could induce the expression of viral an :igens, and suggested that a protein kinase‐C (PKC) may play a major role in the EBV genome activation. We now report further investigations on the role of PKC using 2 selective enzymatic inhibitors [l‐(5‐lsoquinolinylsulfonyl)‐2‐meth‐ylpiperazine] (H‐7) and Staurosporine. We show that these inhibitors can abrogate the inductive effect of TPA or the combination of TPA plus n‐butyrate. The inhibitors have no effisct on induction by calcium ionophores or by viral super‐infection. In this context the effect of verapamil (a specific calcium channel blocker) and of several calmodulin antagonists was investigated. No inhibitory effect of these agents could be demonstrated on any of the induction systems examined. These observations strengthen the idea that in some instances cellular PKC plays a role in the expression of viral antigens; however, alternative regulatory mechanisms cannot be excluded.
Correlation patterns of cancer relative frequencies with some socioeconomic and demographic indicators in Brazil: An ecologic study
Authors: Franco Eduardo L. , Filho Nelson Campos , Villa Luisa L. , Torloni Humberto 
Published: February 2007

AbstractSex‐specific relative frequencies (RF) of oral, esophageal, stomach, colon, rectal, laryngeal, lung, female breast, cervical and penile cancers obtained from a government‐sponsored, nation‐wide data base of histopathological diagnoses were evaluated with respect to all possible inter‐site correlations and with 12 socioeconomic and demographic variables for 23 States in Brazil. Use of bivariate and multivariate methods detected a high positive intercorrelation among RFs of lung, laryngeal and colon cancers regardless of sex. RFs for these 3 sites were also positively correlated with many markers of State development and affluence. Cervical and penile cancers emerged as a distinct subset with respect to their correlation patterns. RFs for these neoplasms were highly (positively) correlated (r = 0.8606, p < 0.001) with each other and exhibited intense negative associations with many of the affluence markers and the former cancer sites. Bivariate correlations generally exhibited a better fit with female‐specific RFs than with data from males which was reflected in the number of strong correlations detected by sex.
Up‐ and down‐regulation of human lymphokine (IL‐2)‐activated killer cell induction by monocytes, depending on their functional state
Authors: Nil Akihiko , Sone Saburo , Utsugi Teruhiro , Yanagawa Hiroaki , Ogura Takeshi 
Published: February 2007

AbstractStudies were made to determine whether freshly isolated monocytes from healthy donors could influence the induction of lymphokine (IL‐2)‐activated killer (LAK) activity. Highly purified lymphocytes ( > 99%) and monocytes (>90%) were isolated by counter‐flaw centrifugal elutriation from peripheral blood. Lymphocytes incubated for 4 days with IL‐2 showed significant LAK activity against natural killer (NK) cell‐resistant target (Daudi) cells, whereas monocytes treated for 4 days with IL‐2 and/or IFN‐γ were not cytotoxic. Under the experimental conditions used, addition of monocytes to the lymphocyte cultures resulted in significant augmentation of the LAK activity, depending on the density of monocytes added. In contrast, monocytes stimulated by lipopolysaccha‐ride (LPS) markedly suppressed LAK activity induced by IL‐2, depending on the dose of LPS added. Similar up‐ and down‐regulations of LAK cell induction by monocytes were observed with 4 lines of human lung cancer cells as targets for LAK activity. Although supernatants from untreated monocytes did not increase LAK induction, supernatants from LPS‐stimulated monocytes suppressed LAK induction. The regulatory role of monocytes could not be replaced by the addition of exogenous interleukin I (IL‐1) or tumor necrosis factor (TNF). Prostaglandin E did not seem to play a regulatory role, since addition of indomethacin did not affect the regulation of LAK cell induction by monocytes‐ These results clearly indicate that human monocytes may cause up‐ or down‐regulation of the expression of IL‐2 induced LAK activity, depending on their functional state.
Human papillomavirus DNA in normal, metaplastic, preneoplastic and neoplastic epithelia of the cervix uteri
Authors: Fuchs P. G. , Girardi F. , Pfister H. 
Published: February 2007

AbstractColposcopically directed cervical punch biopsies from 362 patients were screened by Southern blot hybridization for the presence of DNA of human papillomavirus (HPV) 6, 10, 11, 16, 18, 31 and 33. The biopsies represented original squamous epithelium, Epithelium of metaplastic origin, different stages of cervical intraepithelial neoplasia (CIN) and invasive carcinomas. HPV6/11, 16, 18 and 31 were detected in 2.9% to 13.7% of histologically normal epithelia. HPV6/11 prevailed in CIN I. HPV16 was clearly more abundant than other HPV types in high‐grade CIN and invasive cancers (50%–60%), compared with healthy epithelium. Restriction enzyme cleavage analysis of DNA from primary cancers and corresponding metastases proved the stable association of HPVI6 DNA with invasive tumor cells. Preliminary follow‐up studies of CIN II patients suggested that HPV16‐associated lesions are relatively more likely to persist or to progress. Taken together, the data support the notion of a higher oncogenic potential of HPV16.
Trends in breast cancer incidence and mortality in Denmark, 1943–1982
Authors: Ewertz Marianne , Carstensen Bendix 
Published: February 2007

AbstractThe trend in breast cancer incidence and mortality was examined using data from the Danish Cancer Registry and the national mortality statistics respectively. The study population comprised £5,870 incident cases and 31,817 deaths from breast cancer in Denmark between 1943 and 1982. The incidence rate remained almost constant up to around 1960, whereafter it rose steadily. Comparatively little change was observed in mortality. Possible explanations for the differing trends in incidence and mortality include under‐reporting of breast cancer from death certificates and improvement in survival. A multivariate statistical analysis showed that the increase in incidence was due mostly to a cohort effect, though calendar time may have had a slight effect as well.
Decrease in tumor‐cell attachment and in a 140‐kDa fibronectin receptor correlate with greater expression of multiple 34‐kDa surface proteins and cytoplasmic 54‐kDa components
Authors: Rieber Manuel , Castillo Miguel A. , Ribbbr Mary S. , Irwin Juan C. , Urbina Cecilia 
Published: February 2007

AbstractB 16 melanoma cells attach to matrix‐bound fibronectin but fail to adhere to albumin‐coated surfaces supplemented with soluble fibronectin. Attachment to substratum h also decreased in the presence of an adhesion‐disrupting antibody, or when cells are seeded on substrates poorly adhesive for these cells, such as collagen gels. We have now investigated some of the more general adhesion‐related alterations that occur between flattened and poorly attached cells. Immune blots of octylglucoside extracts with the adhesion‐disrupting IgG revealed a 140‐kDa component in flattened cells, in contrast to the increased detection of a 54‐kDa species in a comparable assay with rounded cells. Surface iodination also showed a decreased external exposure of a 140‐kDa fibronectin binding species and an increased labelling in multiple 34‐kDa protein species, in cells with decreased attachment to substratum. Analysis of 35S‐methionine‐labelled cell aggregates cultured on collagen gels also revealed a decrease in the 140‐kDa region and a greater labelling of multiple 54‐kDa components, compared to the same cells flattened on fibronectin. A change in 54‐ and 34‐kDa species was also teen in matrix‐associated components of rounded cells that failed to attach with soluble fibronectin. Since the 34‐kDa species increase in poorly adherent cells is mainly detected by inclination, and the 54‐kDa species increase in the same celts is partly associated with the corresponding detergent‐insoluble matrices, we propose that these I novel proteins may relate to cell rounding, through a trans membrane modulation involving both surface membrane and cytoskeletal structures.
Resistance to growth of the moloney lymphoma YAC in semi‐syngeneic graft recipients
Authors: Ährlund‐Richthr Lars , Klein Eva 
Published: February 2007

AbstractThe growth of the Motoney‐virus‐induced lymphoma YAC was examined in its strain of origin, A, as well ax in 6 AF1 hybrids, ALF1, AB6F1, ACBF1, AABYF1, AACAF1, and AASWF1, “Hybrid resistance”, i.e., lower frequency of incidence of tumor takes compared to simultaneous A controls, was observed in all of the tested hybrids. The results with the H‐2‐congeneic hybrids AABYF1, AACAF1 and AASWF1, indicated that H‐2 heterozygosity in itself was sufficient to exhibit hybrid resistance against YAC grafts. The A strain and its H‐2 congeneic AABYF1, AACAF1, and AASWF1, hybrids all had similarly low activity in NK assays. Thus, the elevated resistance detected in these H‐2 congeneic hybrids did nat correlate with NK activity. The rejection capacity of the ALF1, AB6F1, and ACBF1, hybrids correlated to their relatively higher NK activity, when compared to the NK low AABYF1, AACAF1, and AASWF1, hybrids. However‐, consideration of quantitative differences in between the hybrids which expressed elevated NK activity (i.e., ALF1, AB6F1, and ACBF1,) shows that the rejection capacity did nat correlate with NK status. The hybrid with the strongest NK effect (ACBF1) was the least resistant to YAC growth (27% palpable tumors), and the hybrid with the weakest NK effect (ALF1) was the most resistant to YAC growth (7.2% palpable tumors).
Radioimmunotherapy of human colon carcinoma by 131I‐labelled monoclonal anti‐cea antibodies in a nude mouse model
Authors: Buchegger F. , Vacca A. , Carrel S. , Schreyer M. , Mach J.‐P. 
Published: February 2007

AbstractA mixture of 3 MAbs directed against 3 different CEA epitopes was radiolabelled with 131I and used for the treatment of a human colon carcinoma transplanted s.c. into nude mice. Intact MAbs and F(ab′)2 fragments were mixed because it had been shown by auto radiography that these 2 antibody forms can penetrate into different areas of the tumor nodule. Ten days after transplantation of colon tumor T380 a single dose of 600 μCi of 131I MAbs was injected i.v. The tumor grafts were well established (as evidenced by exponential growth in untreated mice) and their size continued to increase up to 6 days after radiofabelled antibody injection. Tumor shrinking was then observed lasting for 4–12 weeks. In a control group injected with 600 μCJ of 131I coupled to irrelevant monoclonal IgG, tumor growth was delayed, but no regression was observed. Tumors of mice injected with the corresponding amount of unlabelled antibodies grew like those of untreated mice. Bated on measurements of the effective whole‐body half‐life of injected 131I, the mean radiation dose received by the animals was calculated to be 382 rads for the antibody group and 478 rads for the normal IgG controls. The genetically immunodeficient animals exhibited no increase in mortality, and only limited bone‐marrow toxicity was observed. Direct measurement of radioactivity in mice dissected 1, 3 and 7 days after 131I‐MAb injection showed that 35, 7.2 and 7–2% of injected dose were recovered per gram of tumor, the mean radiation dose delivered to the tumor being thus more than 5,000 rads. These experiments show that therapeutic doses of radioactivity can be selectively directed to human colon carcinoma by i.v. injection of 131I‐labelled anti‐CEA MAbs.
Adhesion characteristics of human melanoma cell lines of varying metastatic potential
Authors: Ormerod E. Jane , Everett Christine A. , Hart Ian R. 
Published: February 2007

AbstractThe adhesive behaviour of a series of human melanoma cell lines, of varying metastatic potential, to basement membrane and stromal components was investigated in vitro. Experimental metastatic propensity was assessed from the number of pulmonary nodules formed after i.v. injection of cells into BALB/c nude mice. All cell lines showed similar kinetics of attachment when tested on plastic, type‐I collagen films, type‐I collagen hydrated gels, fibronectin, laminin type‐IV collagen substrates and bovine aortic endothelial monolayers. Fibro‐nectin‐coated plastic compared to plastic alone produced increased cell attachment and spreading to the same extent in all the cell lines. The melanoma lines attached preferentially to cryostat sections of lung compared to other organs reflecting the pattern of organ involvement of metastasis in vivo. However, no significant quantitative differences in attachment to lung sections were seen between melanoma variants of differing metastatic capacities. Cells labelled with [125I]iodo‐deoxyuridine to determine their initial organ distribution following i.v. injection showed that tumour‐cell arrest was not significantly changed enough to explain the differing metastatic capacities. Thus it appears that adhesive properties of these melanoma cells are not correlated with their capacity to form metastases in vivo.
Immunohistochemical detection of c‐Ha‐ras oncogene p21 product in pre‐neoplastic and neoplastic lesions during hepatocarcinogenesis in rats
Authors: Galand P. , Jacobovitz D. , Alexandre K. 
Published: February 2007

AbstractAn immunohistochemical study of c‐Ha‐ras expression was performed on preneoplastic and neoplastic stages of diethl nitrosamine (DENA)‐induced hepatocarcinogenesis in rats, using an antibody raised against a peptide sequence of the Ha‐ras p21 product. Moderate to high immunostaining intensity was observed in the following hepatocytic lesions: (I) hepato‐cellular carcinomas (14/14) and associated neoplastic nodules (S/S) and foci of phenotypic alterations (35/40) (after 13–20 months of treatment); (2) neoplastic nodules (6/6) and associated foci (42/50) (after 5–9 months); (3) foci (10/10) (after 2 months); and (4) small, slowly growing foci (26/40) found 9 months after treatment with DENA without prior partial hepatectomy, resulting in low number of nodules and no tumor even after IS months. No c‐Ha‐ras p21 was detected immunohistochemically in normal nor in regenerating rat liver. Our result indicate that increased Ha‐ras expression is an early and stable event in liver lesions associated with he‐patocarcinogenesis. They also imply that increased Ha‐ras expression is insufficient (if at all implicated) for inducing fully malignant hepatocyte transformation. It might be indicative of cell populations at an increased transformation risk.
Place of birth and incidence of ocular melanoma in the United States
Authors: Schwartz Stephen M. , Weiss Noel S. 
Published: February 2007

AbstractTo examine whether or not being born in the southern United States is associated with an increased risk of ocular melanoma, the distribution of State of birth of 763 patients (White, not of Spanish origin) with ocular melanoma diagnosed between 1973 and 1984 and identified by 9 population‐based cancer registries participating in the Surveillance, Epidemiology, and End Results program, was compared to the distribution of State of birth of the underlying population of the regions served by these registries. The incidence of ocular melanoma among persons born in the southern United States was nearly the same as that among persons born in the north, after adjustment for age, sex, and residence at diagnosis (Incidence Ratio = 1.1, 95% C.I. 0.8, 1.5). Comparisons of the risk of ocular melanoma between persons born in States with high and low levels of solar radiation yielded similar results, and a trend with increasing solar radiation was observed only among females. These findings do not support the hypothesis that exposure to the sun early in life is a major risk factor for ocular melanoma.
Soft‐tissue sarcomas of the head and neck in adults and children: Experience at a single institution with a review of literature
Authors: Figueiredo Marco T. A. , Marques Lourdes A. , Campos‐Filho Nelson 
Published: February 2007

AbstractNinety‐four soft‐tissue sarcomas of head and neck occurring among 10,700 malignancies, seen during the period 1953–1980 in the head‐and‐neck surgical department, were studied with reference to age, sex, ethnic origin, site, histological type and survival. The objective was to review our own experience with sarcomas in these sites seen in the Department of Pathology, A.C. Camargo Hospital. The predominant site was the head. Fibrosarcoma was the most frequent type in adults and rhabdomyosarcoma, located exclusively in the head, was the most frequent in children.
Generation of monoclonal antibodies reactive with nuclear proteins of human primary breast tumors
Authors: Dinh Bao‐Linh , Tremblay Monique , Paradis Diane , Roy Paul‐Emile , Tetu Bernard 
Published: February 2007

AbstractNuclear proteins were extracted from purified nuclei of human primary breast tumors (BrT) and bladder tumors and of human normal breast, kidney and lymphocytes by enzymatic treatment. SDS‐Polyacrylamide gel electrophoresis of nuclear proteins from breast tumors showed different bands in the molecular weight zones from 25 to 220 kDa which were absent or present only as traces in normal breast tissue. Murine monoclonal antibodies (MAbs) have been produced using nuclear extracts of human primary breast tumors as immunogens. Approximately 2,000 hybridomas were generated from 5 hybridizations. According to their reactivity to BrT nuclear extracts and mammary carcinoma cell line MCF‐7, seven hybridomas were selected and cloned. They were further characterized with histological immunoperoxidase assays of formaldehyde‐fixed BrT paraffin tissue sections. MAb 6A3 particularly gave strong nuclear staining with all BrT specimens while MAb ID8 showed both nuclear and cytoplasmic staining with only some of them. Specimens from mammoplasty did not react with these MAbs. Immunoblotting of BrT nuclear extracts as developed with MAbs 6A3 and ID8 revealed major protein bands with molecular weight of 120 and 130 kDa. The potential use of these MAb‐defined BrT‐related nuclear proteins as markers for human breast cancer was suggested.