bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Iejimalide B, a marine macrolide, on growth and apoptosis in prostate cancer cell lines
Authors: Wang Wei‐Lin Winnie , McHenry Peter , Jeffrey Randy , Schweitzer Dirk , Helquist Paul , Tenniswood Martin 
Published: September 2008

AbstractIejimalide B, a marine macrolide, causes growth inhibition in a variety of cancer cell lines at nanomolar concentrations. We have investigated the effects of Iejimalide B on cell cycle kinetics and apoptosis in the p53+/AR+ LNCaP and p53−/AR− PC‐3 prostate cancer cell lines. Iejimalide B, has a dose and time dependent effect on cell number (as measured by crystal violet assay) in both cell lines. In LNCaP cells Iejimalide B induces a dose dependent G0/G1 arrest and apoptosis at 48 h (as measured by Apo‐BrdU staining). In contrast, Iejimalide B initially induces G0/G1 arrest followed by S phase arrest but does not induce apoptosis in PC‐3 cells. qPCR and Western analysis suggests that Iejimalide B modulates the steady state level of many gene products associated with cell cycle (including cyclins D, E, and B and p21waf1/cip1) and cell death (including survivin, p21B and BNIP3L) in LNCaP cells. In PC‐3 cells Iejimalide B induces the expression of p21waf1/cip1, down regulates the expression of cyclin A, and does not modulate the expression of the genes associated with cell death. Comparison of the effects of Iejimalide B on the two cell lines suggests that Iejimalide B induces cell cycle arrest by two different mechanisms and that the induction of apoptosis in LNCaP cells is p53‐dependent. J. Cell. Biochem. 105: 998–1007, 2008. © 2008 Wiley‐Liss, Inc.
BCCIP associates with the receptor protein tyrosine phosphatase PTPµ
Authors: Phillips‐Mason Polly J. , Mourton Tracy , Major Denice L. , Brady‐Kalnay Susann M. 
Published: September 2008

AbstractThe receptor protein tyrosine phosphatase PTPµ belongs to a family of adhesion molecules that contain cell–cell adhesion motifs in their extracellular segments and catalytic domains within their intracellular segments. The ability of PTPµ both to mediate adhesion and exhibit enzymatic activity makes PTPµ an excellent candidate to transduce signals in response to cell–cell adhesion. In an effort to identify downstream signaling partners of PTPµ, we performed a modified yeast two‐hybrid screen using the first tyrosine phosphatase domain of PTPµ as bait. We isolated an interacting clone encoding BRCA2 and CDKN1A interacting protein (BCCIP) from a HeLa cell library. BCCIP is a p21 and BRCA2 interacting protein that has been shown to play roles in both cell cycle arrest and DNA repair. In this manuscript, we confirm the interaction between BCCIP and PTPµ identified in yeast using in vitro biochemical studies and characterize BCCIP as a PTPµ binding protein. We demonstrate that BCCIP is phosphorylated by the Src tyrosine kinase and dephosphorylated by the PTPµ tyrosine phosphatase in vitro. Furthermore, we show that BCCIP is required for both the permissive and repulsive functions of PTPµ in neurite outgrowth assays, suggesting BCCIP and PTPµ are in a common signal transduction pathway. J. Cell. Biochem. 105: 1059–1072, 2008. © 2008 Wiley‐Liss, Inc.
Characterization of TRIM31, upregulated in gastric adenocarcinoma, as a novel RBCC protein
Authors: Sugiura Takeyuki , Miyamoto Kentaro 
Published: September 2008

AbstractTo explore the molecules associated with gastric adenocarcinoma, we used the gene expression profile database of various human tissues and identified TRIM31 upregulated in both patients with chronic gastritis and stomach cancer. TRIM31 is a new member of RBCC proteins composed of RING finger, B‐box and coiled‐coil domains. We characterized TRIM31 biochemically and found it possess properties in common with other RBCC proteins, such as occurrence of alternative splicing transcripts, in vitro autoubiquitylating activity and a tendency to homo‐oligomerize. The primary localization site of TRIM31 is the cytoplasm but some fraction is potentially associated with the mitochondria. TRIM31 overexpression suppresses colony formation of HCT116 cells while knockdown of its expression with short interfering RNAs (siRNAs) consistently tends to enhance growth of AsPC‐1 cells slightly. Thus, TRIM31 is a characteristic RBCC protein with the ability to regulate cell proliferation negatively and may be a potential biomarker of gastric cancer as it is overexpressed from the early stage of gastric carcinogenesis. J. Cell. Biochem. 105: 1081–1091, 2008. © 2008 Wiley‐Liss, Inc.
Retroviral vector silencing during iPS cell induction: An epigenetic beacon that signals distinct pluripotent states
Authors: Hotta Akitsu , Ellis James 
Published: September 2008

AbstractRetroviral vectors are transcriptionally silent in pluripotent stem cells. This feature has been potently applied in studies that reprogram somatic cells into induced pluripotent stem (iPS) cells. By delivering the four Yamanaka factors in retroviral vectors, high expression is obtained in fibroblasts to induce the pluripotent state. Partial reprogramming generates Class I iPS cells that express the viral transgenes and endogenous pluripotency genes. Full‐reprogramming in Class II iPS cells silences the vectors as the endogenous genes maintain the pluripotent state. Thus, retroviral vector silencing serves as a beacon marking the fully reprogrammed pluripotent state. Here we review known silencer elements, and the histone modifying and DNA methylation pathways, that silence retroviral and lentiviral vectors in pluripotent stem cells. Both retroviral and lentiviral vectors are influenced by position effects and often exhibit variegated expression. The best vector designs facilitate full‐reprogramming and subsequent retroviral silencing, which is required for directed‐differentiation. Current retroviral reprogramming methods can be immediately applied to create patient‐specific iPS cell models of human disease, however, future clinical applications will require novel chemical or other reprogramming methods that reduce or eliminate the integrated vector copy number load. Nevertheless, retroviral vectors will continue to play an important role in genetically correcting patient iPS cell models. We anticipate that novel pluripotent‐specific reporter vectors will select for isolation of high quality human iPS cell lines, and select against undifferentiated pluripotent cells during regenerative medicine to prevent teratoma formation after transplantation. J. Cell. Biochem. 105: 940–948, 2008. © 2008 Wiley‐Liss, Inc.
bFGF induces S1P1 receptor expression and functionality in human pulmonary artery smooth muscle cells
Authors: Birker‐Robaczewska Magdalena , Studer Rolf , Haenig Bénédicte , Menyhart Katalin , Hofmann Silke , Nayler Oliver 
Published: September 2008

AbstractSphingosine‐1‐phosphate (S1P), acting through five closely related G‐protein coupled receptors termed S1P1–5, has recently emerged as a possible regulator of smooth muscle cell (SMC) physiology with the potential to induce contraction, proliferation and stress fiber formation. In the present study, real‐time quantitative PCR was used to determine the expression patterns of S1P receptor subtypes in human primary pulmonary artery smooth muscle cells (PASMC). We report here that subconfluent PASMC express predominantly S1P2 and S1P3 receptors and we show that S1P1 receptor mRNA levels are significantly up‐regulated following basic fibroblast growth factor (bFGF) treatment. As a consequence, increased responsiveness, as measured by impedance and ERK1/2 phosphorylation, was observed upon stimulation with a specific S1P1 receptor agonist SEW2871. We therefore demonstrate, for the first time, that a growth factor that was previously shown to be involved in physiological and pathological changes of SMC function induced S1P1 receptor expression and we propose that S1P1 receptor up‐regulation could contribute to vascular remodeling. J. Cell. Biochem. 105: 1139–1145, 2008. © 2008 Wiley‐Liss, Inc.
Simulation of DNA electrophoresis in systems of large number of solvent particles by coarse‐grained hybrid molecular dynamics approach
Authors: Wang Rong , Wang Jian‐Sheng , Liu Gui‐Rong , Han Jongyoon , Chen Yu‐Zong 
Published: September 2008

AbstractSimulation of DNA electrophoresis facilitates the design of DNA separation devices. Various methods have been explored for simulating DNA electrophoresis and other processes using implicit and explicit solvent models. Explicit solvent models are highly desired but their applications may be limited by high computing cost in simulating large number of solvent particles. In this work, a coarse‐grained hybrid molecular dynamics (CGH‐MD) approach was introduced for simulating DNA electrophoresis in explicit solvent of large number of solvent particles. CGH‐MD was tested in the simulation of a polymer solution and computation of nonuniform charge distribution in a cylindrical nanotube, which shows good agreement with observations and those of more rigorous computational methods at a significantly lower computing cost than other explicit‐solvent methods. CGH‐MD was further applied to the simulation of DNA electrophoresis in a polymer solution and in a well‐studied nanofluidic device. Simulation results are consistent with observations and reported simulation results, suggesting that CGH‐MD is potentially useful for studying electrophoresis of macromolecules and assemblies in nanofluidic, microfluidic, and microstructure array systems that involve extremely large number of solvent particles, nonuniformly distributed electrostatic interactions, bound and sequestered water molecules. © 2008 Wiley Periodicals, Inc. J Comput Chem 2009
Application of a polarity parameter model to the separation of fat‐soluble vitamins by reversed‐phase HPLC
Authors: Herrero‐Martínez José Manuel , Izquierdo Pere , Sales Joaquim , Rosés Martí , Bosch Elisabeth 
Published: September 2008

AbstractThe retention behavior of a series of fat‐soluble vitamins has been established on the basis of a polarity retention model: log k = (log k)0 + p (PmN – PsN), with p being the polarity of the solute, PmN the mobile phase polarity, and (log k)0 and PmN two parameters for the characterization of the stationary phase. To estimate the p‐values of solutes, two approaches have been considered. The first one is based on the application of a QSPR model, derived from the molecular structure of solutes and their log Po/w, while in the second one, the p‐values are obtained from several experimental measurements. The quality of prediction of both approaches has also been evaluated, with the second one giving more accurate results for the most lipophilic vitamins. This model allows establishing the best conditions to separate and determine simultaneously some fat‐soluble vitamins in dairy foods.
GC‐MS fingerprints for discrimination of Ligusticum chuanxiong from Angelica
Authors: Yang Baijuan , Chen Junhui , Lee Frank Sen‐Chun , Wang Xiaoru 
Published: September 2008

AbstractA GC‐MS fingerprinting technique based on the essential oil components has been developed for the discrimination of chuanxiong against Chinese Angelica (Angelica sinensis (Oliv.) Diels) or other herbs with similar compositions. The analytical performance of four different extraction methods for the separation of essential oil components have been compared and these include: ultrasound‐assisted extraction (UAE), supercritical fluid extraction (SFE), Soxhlet extraction (SHE) and hydro‐distillation extraction (HDE). The results showed that UAE was the most effective extraction method, and the operational parameters of UAE were optimized. 3‐Butylphthalide, Z‐butylidenephthalide, senkyunolide I, senkyunolide H, E‐butylidenephthalide, senkyunolide A, neocnidilide, Z‐ligustilide and E‐ligustilide were tentatively identified in chromatograms of chuanxiong based on their GC‐EI‐MS data. Similarity coefficient calculations based on correlation methods have been performed on the GC‐MS fingerprints. Using an authentic standard Chuanxiong as the reference, the similarity coefficients between the standard and all other chuanxiong samples ranged from 0.90 to 1.0 (with 1.0 being the perfect match), which as a group can be readily separated from the Angelica samples for which the similarity index against the chuanxiong standard ranged from 0.75 to 0.77. Conversely, when an authentic Angelica standard was used as the reference, the respective similarity coefficients fall in the range of 0.70–0.75 and 0.98–1.00 for the chuanxiong and Angelica sample groups. Our results thus demonstrate that the fingerprinting technique developed in the study can indeed discriminate the two herbs with high reliability.
GC‐MS of volatile components of Schisandra chinensis obtained by supercritical fluid and conventional extraction
Authors: Wang Longhu , Chen Yangsheng , Song Yanting , Chen Yong , Liu Xuesong 
Published: September 2008

AbstractIn this paper, the volatile compounds of Schisandra chinensis obtained by different extraction techniques including supercritical fluid extraction (SFE), steam distillation (SD), Soxhlet extraction (SE) and ultrasound‐assisted extraction (UAE) were investigated for the first time. The sample preparation procedure for GC‐MS analysis of the volatile compounds was optimized and then 37, 45, 27 and 37 compounds were identified in the samples obtained by SFE, SD, SE and UAE methods, respectively. As the therapeutic effect of the traditional Chinese medicine is usually based on multifarious essential components or the combination of them instead of only one component, the volatile compounds were compared in groups with the extracts by SE, SD and UAE. This would be more reasonable to evaluate the effects of an alternative technique to extracting multifarious essential components. Among the identified components in the SFE extract, 32 compounds were the same as that by three conventional methods, accounting for 90.5% of the volatile compounds identified. However, as the volatile compounds were classed into groups, it was easy to see that the Schisandra chinensis oil extracted by SFE was made up largely of aromatics and sesquiterpenoids (52.1 and 27.6%, respectively), with less amounts of monoterpenoids and other compounds, distinguishing SFE from the conventional extractions.
Immobilization of cellulose phenylcarbamate onto silica gelviain termolecular polycondensation of triethoxysilyl groups introduced with (3‐glycidoxypropyl)triethoxysilane
Authors: Tang Shouwan , Okamoto Yoshio 
Published: September 2008

AbstractCellulose 3,5‐dimethylphenylcarbamate was successfully immobilized onto bare silica gel for HPLC through the intermolecular polycondensation of triethoxysilyl groups, which were introduced onto the cellulose derivativeviaepoxide ring‐opening reaction under acidic conditions. The immobilized‐type chiral packing material (CPM) exhibited high chiral recognition ability and could be used with various eluents, which are incompatible with the conventional CPMs prepared by coating the derivative onto silica gel.
Kinetic Modeling of Normal ATRP, Normal ATRP with [CuII]0, Reverse ATRP and SR&NI ATRP
Authors: Tang Wei , Matyjaszewski Krzysztof 
Published: September 2008

AbstractThe kinetics of various ATRP systems, including normal ATRP, normal ATRP in the presence of initially‐added CuII, reverse ATRP and SR&NI ATRP were modeled using Predici software. The instantaneous kinetic chain length was introduced for ATRP and was used for the prediction of control over polymerization. Equations were derived to estimate the radical concentration at the quasi‐steady‐state. Normal ATRP experiences a continuous decrease of radical concentration leading to a decrease of polymerization rate; in contrast, SR&NI ATRP undergoes a continuous increase in radical concentration, leading to an increase of the polymerization rate. All of these ATRP methods can afford a relatively fast polymerization rate and retain good polymerization control.magnified image
Validation of fast MR thermometry at 1.5 T with gradient‐echo echo planar imaging sequences: phantom and clinical feasibility studies
Authors: Cernicanu Alexandru , Lepetit‐Coiffe Matthieu , Roland Joerg , Becker Christoph D. , Terraz Sylvain 
Published: September 2008

AbstractThe purpose of this work was to validate in phantom studies and demonstrate the clinical feasibility of MR proton resonance frequency thermometry at 1.5 T with segmented gradient‐echo echo planar imaging (GRE‐EPI) sequences during liver tumour radiofrequency (RF) ablation. Classical GRE acquisitions and segmented GRE‐EPI acquisitions were performed at 1.5 T during simultaneous RF heating with an MR‐compatible RF electrode placed in an agar gel phantom. Temperature increments were calculated and compared with four optical temperature probe measurements using Bland– Altman analysis. In a preliminary clinical feasibility study, the rapid GRE‐EPI sequence (echo train length = 13) was used for MR temperature monitoring of RF ablation of liver tumours in three patient procedures. For phantom experiments, the Bland–Altman mean of differences between MR and optical probe temperature measurements was <0.4°C, and the 95% limits of agreement value was <1.4°C. For the in vivo studies, respiratory‐triggered GRE‐EPI acquisitions yielded a temperature accuracy of 1.3 ± 0.4°C (acquisition time = 0.6 s/image, spatial coverage of three slices/respiratory cycle). MR proton resonance frequency thermometry at 1.5 T yields precise and accurate measurements of temperature increment with both classical GRE and rapid GRE‐EPI sequences. Rapid GRE‐EPI sequences minimize intra‐scan motion effects and can be used for MR thermometry during RF ablation in moving organs. Copyright © 2008 John Wiley & Sons, Ltd.
Differential Ability Scales – Second Edition (neuro)psychological predictors of math performance for typical children and Children with Math Disabilities
Authors: Hale James B. , Fiorello Catherine A. , Dumont Ron , Willis John O. , Rackley Christopher , Elliott Colin 
Published: September 2008

AbstractConcerns about the ability‐achievement discrepancy method for specific learning disability (SLD) determination led to alternative research‐based methods, such as failure to respond to intervention. Neither of these regulatory methods address the statutory SLD definition, which explicitly includes a deficit in basic psychological processes. Examining neuropsychological processing differences among typical children and children with math SLD, commonality analyses revealed that Differential Ability Scales – Second Edition (DAS‐II) predictors accounted for more achievement variance in typical children (46% to 58%) than in children with math SLD (33% to 50%), with substantial loss of predictive validity when General Conceptual Ability was used instead of subcomponent scores. Results suggest that differences in typical predictor‐outcome relationships may provide a foundation for developing specific cognitive and academic interventions for children with math SLD. © 2008 Wiley Periodicals, Inc.
Assessing executive functions: A life‐span perspective
Authors: Reynolds Cecil R. , MacNeill Horton Arthur 
Published: September 2008

AbstractDespite many disagreements on the utility of neuropsychological applications in schools, executive function measures have been found to be useful across a variety of areas and ages. In addition, many disagreements are extant in discussions of the maturational course of the development of executive functioning abilities that are dependent on functional capacity of the human brain, including the frontal lobes, among other brain areas. In part, these controversies are related to a dearth of standardized functional assessments of executive functioning abilities across wide age spans. This article describes several recent measures of executive functioning and uses life‐span data from these assessments to project maturational periods of specific executive functions. Clinical implications of these results for school psychology assessment, rehabilitation of brain‐injured school‐aged children, and forensic practice of school psychology are posited. © 2008 Wiley Periodicals, Inc.
Behavioral profiles of children with epilepsy: Parent and teacher reports of emotional, behavioral, and educational concerns on the BASC‐2
Authors: Titus Jeffrey B. , Kanive Rebecca , Sanders Sarah J. , Blackburn Lynn Bennett 
Published: September 2008

AbstractThe Behavior Assessment Scale for Children – Second Edition (BASC‐2) was administered to 108 parents and 37 teachers of children with epilepsy (mean age of 10.6 years; 51% female, 49% male). Results demonstrated high mean scores on the Atypicality, Attention Problems, Withdrawal, and Adaptive Skills scales and a high percentage of At‐Risk or Clinically Significant scores on the majority of scales. Ratings were correlated with level of functioning, age of onset, and number of antiepileptic medications. In addition, children with poor seizure control had higher ratings on the Depression, Somatization, and Withdrawal scales, and children with temporal lobe seizures had higher scores on the Depression scale. These results confirm a high rate of behavioral concerns in children with epilepsy. © 2008 Wiley Periodicals, Inc.
Luminescence from self‐trapped excitons in BaFI and BaFBr1–xIx crystals
Authors: Ohnishi Akimasa , Kan'no Ken‐ichi 
Published: September 2008

AbstractLuminescence from BaFI and BaFBr1–x Ix crystals has been investigated using vacuum ultraviolet photons from synchrotron radiation in the temperature range of 10–300 K. In BaFI, two kinds of luminescence bands are observed under band‐to‐band excitation: one is the 4.52 eV band appearing at low temperatures, and the other is the 3.44 eV band appearing as the 4.52 eV band weakens with increasing temperature. From the excitation spectra, the 4.52 eV band is attributed to the radiative decay of a self‐trapped exciton (STE), and the 3.44 eV band to the extrinsic luminescence coming from an STE trapped by an I– vacancy. The change of luminescence spectra studied in BaFBr1–x Ix suggests that the STE configuration of on‐center type is responsible for the 4.52 eV intrinsic band, and the off‐center instability hardly occurs in BaFI. The temperature dependence of the 4.52 eV and 3.44 eV band intensities is well explained by assuming an STE relaxation mechanism whereby the STE relaxes through phonon‐assisted tunneling and hopping diffusion processes at high temperatures. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Phase transition, and elastic and thermodynamic properties of InN derived from first‐principles and the quasi‐harmonic Debye model
Authors: Peng Feng , Han Yunxia , Fu Hongzhi , Cheng Xinlu 
Published: September 2008

AbstractThe transition phase of InN from wurtzite (WZ) structure to rocksalt (RS) structure is investigated via the minimization of the total energy within Local Density Approximation (LDA) based on Density Functional Theory (DFT), the calculated equilibrium volume are in agreement with available experimental value and other calculated data. In the meantime, the thermodynamic properties of different phase under high temperature and high pressure are investigated applying nonempirical Debye model combining with the first principle theory in the quasi‐harmonic approximation. The transition phase from WZ structure to RS structure occurs at the pressure of 10.1 GPa, which agrees well with experimental value. The evaluated equilibrium volume using this model agrees with the value obtained from ab intio and from experiment. The results demonstrate that this method can provide reliable predictions for the temperature and pressure dependence of these quantities such as the equation of state (EOS), the isothermal bulk modulus, the heat capacity, and the thermal expansion in detail. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)