bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minor changes in calculated creatinine clearance and anion‐gap are associated with tenofovir disoproxil fumarate‐containing highly active antiretroviral therapy
Authors: Winston A , Amin J , Mallon PWG , Marriott D , Carr A , Cooper DA , Emery S 
Published: January 2006

BackgroundTenofovir disoproxil fumarate (Tenofovir DF, TDF), the first nucleotide reverse transcriptase inhibitor approved for the treatment of HIV disease, has been associated with renal dysfunction in isolated cases. The aim of this study was to assess changes in renal parameters in individuals receiving TDF‐ and non‐TDF‐containing highly active antiretroviral therapy (HAART).MethodsAll individuals on HAART attending our clinic were included in the analysis. Time‐weighted changes in serum creatinine, calculated creatinine clearance (CCrCl) and anion‐gap were assessed for individuals on TDF‐ and non‐TDF HAART.ResultsOf 948 individuals on HAART, 290 (31%) and 618 (65%) were on TDF‐ and non‐TDF HAART, with 40 (4%) having ceased TDF HAART. Baseline values for serum creatinine, CCrCl and anion‐gap were similar for those on TDF‐ and non‐TDF HAART. In a multivariate analysis, statistically significant differences were observed in time‐weighted change from baseline in anion‐gap and CCrCl between individuals on TDF‐ and non‐TDF HAART [mean difference in change between groups: anion‐gap 0.78 mmol/L (standard error, 0.19) and CCrCl−6.80 (standard error 2.2); P=0.005 and P=0.032, respectively] after adjusting for baseline anion‐gap and CCrCl, respectively. Two cases of TDF‐associated renal failure were observed.ConclusionOvert renal failure with TDF HAART is rare. However, subtle but statistically significant changes in anion‐gap and CCrCl were observed which were associated with TDF HAART. These parameters may be of use in monitoring individuals on HAART.
Microsatellite loci for the Australian field cricket Teleogryllus oceanicus and their cross‐utility in Teleogryllus commodus
Authors: BEVERIDGE MAXINE , SIMMONS LEIGH W. 
Published: January 2006

AbstractMicrosatellite loci were isolated from the Australian field cricket Teleogryllus oceanicus. Seven polymorphic loci were found with an observed number of alleles ranging from eight to 17 and observed heterozygosities between 0.26 and 0.94. One locus was found to be X‐linked. These seven loci were also tested for amplification and polymorphism in the congeneric species Teleogryllus commodus. The loci will be used for paternity studies in these species.
Development and mapping of a public reference set of SSR markers in Lolium perenne L.
Authors: JENSEN LOUISE BACH , MUYLLE HILDE , ARENS PAUL , ANDERSEN CLAUS H. , HOLM PREBEN BACH , GHESQUIERE MARC , JULIER BERNADETTE , LÜBBERSTEDT THOMAS , NIELSEN KLAUS K. , RIEK JAN DE , ROLDÁN‐RUIZ ISABEL , ROULUND NIELS , TAYLOR CHRIS , VOSMAN BEN , BARRE PHILIPPE 
Published: January 2006

AbstractWe report on the characterization and mapping of 76 simple sequence repeat (SSR) markers for Lolium perenne. These markers are publicly available or obtained either from genomic libraries enriched for SSR motifs or L. perenne expressed sequence tag (EST) clones. Four L. perenne mapping populations were used to map the SSR markers. A consensus linkage map of the four mapping populations containing 65 of the SSR markers is presented, together with primer information and a quality score indicating the usefulness of the SSR marker in different populations. The SSR markers identified all seven L. perenne linkage groups.
Isolation and characterization of 10 microsatellite loci in the Ponerinae ant Pachycondyla luteipes (Hymenoptera; Formicidae)
Authors: TAKAHASHI J. , KIKUCHI T. , OHNISHI H. , TSUJI K. 
Published: January 2006

AbstractWe developed 10 polymorphic microsatellite loci for the Ponerinae ant Pachycondyla luteipes using the magnetic particle method. Nine of the 10 loci tested were highly polymorphic, having four to eight alleles in P. luteipes with expected heterozygosity of 0.38–0.73, and also appeared to be applicable to other related species such as Pachycondyla chinensis. These loci can be used to test the hypotheses on the population genetic structures, such as if this ant is unicolonial or not.
Isolation and characterization of microsatellite loci from chub Leuciscus cephalus (Pisces: Cyprinidae)
Authors: LARNO VALÉRIE , LAUNEY SOPHIE , DEVAUX ALAIN , LAROCHE JEAN 
Published: January 2006

AbstractTwelve microsatellite markers were isolated from chub Leuciscus cephalus Linné, 1766 (Cyprinidae), a freshwater fish widely distributed in Europe. We assessed the level of genetic diversity for these loci in 24 individuals sampled in the Rhone river watershed. Nine loci were polymorphic, displaying from two to 13 alleles per locus with expected heterozygosity ranging from 0.160 to 0.887. Simultaneous migration of different polymerase chain reaction products was developed for routine analysis of L. cephalus populations.
Isolation and characterization of microsatellites in the woody shrub, Calothamnus quadrifidus (Myrtaceae)
Authors: ELLIOTT C. P. , BYRNE M. 
Published: January 2006

AbstractMicrosatellite loci were developed for genetic analysis of the bird pollinated woody shrub Calothamnus quadrifidus. A genomic library was constructed and screened with dinucleotide and trinucleotide repeat sequences. Ten dinucleotide microsatellite markers were developed, and polymorphism in a population of C. quadrifidus was investigated for six of these markers, which showed an average of 12.7 alleles per locus. Mendelian inheritance of the loci was confirmed through analysis of open pollinated progeny arrays of 10 plants. These loci will be used to study gene flow patterns between isolated populations of this species in southwest Western Australia.
Isolation and characterization of two dinucleotide and four tetranucleotide polymorphic microsatellite loci in the Iberian midwife toad Alytes cisternasii
Authors: GONÇALVES H. , PEREIRA R. , ROWE G. , BEEBEE T. , FERRAND N. 
Published: January 2006

AbstractThe Iberian midwife toad Alytes cisternasii is an endemic species of the southwestern and central parts of the Iberian Peninsula. Allozyme analysis of Iberian midwife toads indicated no inter‐ or intrapopulation variation and a more sensitive method is required to quantify genetic differentiation and gene flow between populations of this species. We describe here the development of primers for the amplification by the polymerase chain reaction (PCR) of six polymorphic microsatellite loci in A. cisternasii.
Isolation of polymorphic tetranucleotide microsatellite markers for the black‐bellied seedcracker (Pyrenestes ostrinus)
Authors: DELANEY KATHLEEN SEMPLE , PIRES DEBRA , HOLDER KAREN , BARDELEBEN CAROLYNE , SMITH THOMAS 
Published: January 2006

AbstractWe have isolated 10 polymorphic microsatellite markers for the black‐bellied seedcracker (Pyrenestes ostrinus) from genomic libraries enriched either for (AAGG)n or (ATCT)n repetitive elements and characterized them in 39 individuals. The number of alleles ranged from two to 27 per locus with the observed heterozygosity ranging from 0.38 to 0.94. These markers will be useful for analysis of questions concerning parentage and population genetic structure.
Sixteen microsatellite loci from Halesia tetraptera (Styracaceae)
Authors: MEEROW ALAN W. , ROH MARK S. , SCHNELL RAYMOND J. 
Published: January 2006

AbstractSixteen microsatellite loci isolated from Halesia tetraptera are described. All 16 are polymorphic, with four to 13 alleles across 15–19 members of a single half‐sib family. Heterozygosity ranged from 0.067 to 1. One locus departs significantly from Hardy–Weinberg equilibrium in our test family. The test population shows significant heterozygote deficiency at this and two other loci. Thirteen loci exhibit significant linkage disequilibrium. These loci will be utilized in paternity analyses of geographically diverse half‐sib families.
A set of microsatellite markers for an endangered arboreal marsupial, Leadbeater's possum
Authors: HANSEN B. D. , SUNNUCKS P. , BLACKET M. , TAYLOR A. C. 
Published: January 2006

AbstractLeadbeater's possum is a small, cryptic nongliding petaurid, which is threatened by habitat loss due to the collapse of large hollow‐bearing trees and ongoing timber production in Victoria's central highlands. The recent decline of this species and the difficulties faced by researchers collecting field data has prompted the development of a suite of polymorphic microsatellite markers for application to the continuing conservation of the Leadbeater's possum.
Isolation and characterization of microsatellite loci in the threatened brook lamprey Lethenteron sp. N
Authors: TAKESHIMA H. , YOKOYAMA R. , NISHIDA M. , YAMAZAKI Y. 
Published: January 2006

AbstractA microsatellite‐enriched genomic library was obtained for the threatened brook lamprey, Lethenteron sp. N, and from it 16 dinucleotide markers were successfully isolated and characterized. These markers were found to have between 1 and 6 alleles with heterozygosity ranging from zero to 0.79. Cross‐species amplification was successful, with eight loci amplifying products in the counterpart cryptic species, Lethenteron sp. S, as well as 15 loci in Lethenteron japonicum. One out of 10 primer pairs previously reported also amplified products in all the three species.
Development of new polymorphic microsatellite markers for the New World screw‐worm Cochliomyia hominivorax (Diptera: Calliphoridae)
Authors: TORRES TATIANA TEIXEIRA , DE AZEREDO‐ESPIN ANA MARIA LIMA 
Published: January 2006

AbstractIn this report, we describe the development of seven new polymorphic microsatellite markers for Cochliomyia hominivorax, a parasitic insect pest of primary agricultural and veterinary importance throughout the Neotropics. The number of alleles found ranged from 3 to 13 per locus, with the expected heterozygosity ranging from 0.4220 to 0.9045. The across‐taxa amplification of some of these new microsatellite loci was successful in four additional Calliphoridae species. In combination with the 10 polymorphic microsatellite markers previously described, the markers developed here should provide a high resolution for assessing the fine‐scale genetic structure of New World screw‐worms.
Development of microsatellite markers for mango (Mangifera indica L.)
Authors: DUVAL M. F. , BUNEL J. , SITBON C. , RISTERUCCI A. M. 
Published: January 2006

AbstractMicrosatellite markers for mango (Mangifera indica L.) were developed using a genomic library enriched for (GA)n and (GT)n dinucleotide repeats. A subset of 41 positive clones was sequenced and primers were designed. Twenty‐eight primer pairs produced polymorphic amplification products for a diversity sample including 15 mango cultivars and two accessions from the related species Mangifera laurina and Mangifera applanata. Nineteen simple sequence repeat (SSR) loci with clear scorable patterns were chosen to study diversity in the mango germplasm bank of Guadalupe (FWI). The number of alleles ranged from three to 13 with observed levels of heterozygosity ranging from 0.059 to 0.857.
Isolation of polymorphic tetranucleotide microsatellite markers for the masked flowerpiercer Diglossa cyanea
Authors: BARDELEBEN CAROLYNE , GRAY MELISSA M. 
Published: January 2006

AbstractWe have isolated 11 polymorphic microsatellite markers for the masked flowerpiercer Diglossa cyanea from a genomic library enriched for (AAGG)n repetitive elements. The number of alleles ranged from eight to 16 per locus with the observed heterozygosity ranging from 0.64 to 1.00. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation.
Eleven new highly polymorphic microsatellite loci in the yellow croaker,Pseudosciaena crocea
Authors: AN H. S. , CHO K. C. , PARK J. Y. 
Published: January 2006

AbstractWe developed 11 new microsatellite markers inPseudosciaena croceaby screening an enriched genomic library using nonradioactive polymerase chain reaction (PCR) techniques. All loci were found to be polymorphic with an average of 14.9 alleles per locus (range four to 30). The mean observed and expected heterozygosities were 0.86 (range 0.57–1.00) and 0.90 (range 0.62–0.98), respectively. Four loci showed significant Hardy–Weinberg disequilibrium. The high variabilities revealed in this study suggest that these microsatellite loci should provide useful markers for population genetic studies ofP. crocea.
Isolation of polymorphic tetranucleotide microsatellite markers for the skink Mabuya affinis
Authors: BARDELEBEN CAROLYNE , MOORE RACHAEL L. , GRAY MELISSA M. 
Published: January 2006

AbstractWe isolated 10 polymorphic microsatellite markers for the skink Mabuya affinis from genomic libraries enriched for (AAGG)n and (AAAG)n repetitive elements. The number of alleles ranged from eight to 13 per locus with the observed heterozygosity ranging from 0.60 to 0.92. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation.
Isolation and characterization of novel dinucleotide repeat microsatellites in the Jamaican click beetlePyrophorus plagiophthalamus(Coleoptera: Elateridae)
Authors: VELEZ S. , FEDER J. L. 
Published: January 2006

AbstractWe report 10 polymorphic microsatellite loci for the Jamaican click beetle,Pyrophorus plagiophthalamus. A survey of 36 individuals from three populations in Jamaica showed that these are highly variable, with three to 17 alleles per locus. Observed heterozygosity ranged from 0.250 to 0.917, and mean heterozygosity from 0.601 to 0.747. Most loci were in Hardy–Weinberg equilibrium, although excess of homozygotes was observed in four tests (out of 20), suggesting the possibility of null alleles. Significant linkage disequilibrium was observed for only one pair. These newly developed markers will be useful in understanding the population structure of click beetles in Jamaica, and in identifying possible selective factors responsible for bioluminescent colour variation.
Ten polymorphic microsatellite markers developed in the masson pine moth Dendrolimus punctatus Walker (Lepidoptera: Lasiocampidae)
Authors: JI YA‐JIE , HUA YUE‐PING , LIU YU‐DI , LIU HUA‐TAO , SHI CHENG‐MIN , LI DIAN‐MO , ZHANG DE‐XING 
Published: January 2006

AbstractA set of 10 polymorphic di‐ and trinucleotide microsatellite loci were developed for the forestry pest insect, masson pine moth, Dendrolimus punctatus Walker. The expected heterozygosity at these loci ranges from 0.285 to 0.859, and the observed allele numbers from five to 19. Cross species amplification of these loci in four other congeneric pine moth species indicates variable levels of loci conservation and thus cross‐applicability. Therefore, the microsatellite loci reported here should be useful for population genetic and other related studies in the masson pine moth and other closely related species.
Development of polymorphic markers for Cirsium arvense, Canada thistle, and their amplification in closely related taxa
Authors: SLOTTA TRACEY A. BODO , FOLEY MICHAEL E. , HORVATH DAVID 
Published: January 2006

AbstractSuppression of invasive Canada thistle, Cirsium arvense, with biological control agents has stalled because introduced agents were not host‐specific. To aid in the development of more effective management strategies, molecular markers are needed to examine the genetic structure of Canada thistle populations. Microsatellite (simple sequence repeat) markers were developed and intersimple sequence repeat (ISSR) markers were tested for North American populations. An average of nine polymorphic alleles per microsatellite locus and 11 per ISSR locus were detected. These will be used to examine the genetic structure of C. arvense in the northern Great Plains and their transferability to endemic Cirsium spp.
Isolation and characterization of 12 tetra‐repeated microsatellite loci from the Formosan grass lizard (Takydromus formosanus)
Authors: LIN SI‐MIN , WANG CHAO‐JUN , HSU YU‐CHENG , LUE KUANG‐YANG , LI SHOU‐HSIEN 
Published: January 2006

AbstractTwelve tetra‐repeated microsatellite loci were developed for the island‐endemic grass lizard Takydromus formosanus (Squamata: Lacertidae). We characterized these loci by genotyping 51 individuals sampled from six localities. The number of alleles per locus ranged between 12 and 23, whereas the observed heterozygosity ranged between 0.686 and 0.941. None of these loci showed gametic disequilibrium or sex linkage. With such high polymorphism, we believe that these loci should be suitable for fine‐scaled analysis of population structure, hybridizations or individual identification of grass lizards.
Isolation and characterization of microsatellite markers in Fraser fir (Abies fraseri)
Authors: JOSSERAND S. A. , POTTER K. M. , JOHNSON G. , BOWEN J. A. , FRAMPTON J. , NELSON C. D. 
Published: January 2006

AbstractWe describe the isolation and characterization of 14 microsatellite loci from Fraser fir (Abies fraseri). These markers originated from cloned inserts enriched for DNA sequences containing tandem di‐ and tri‐nucleotide repeats. In total, 36 clones were selected, sequenced and evaluated. Polymerase chain reaction (PCR) primers for 14 of these sequences consistently produced simple PCR profiles and were found to be polymorphic among 13 Fraser fir samples. In addition, more than half of these loci were found to amplify a wide range of samples from several Abies taxa.
Six microsatellite loci from the deep‐sea coral Corallium lauuense (Octocorallia: Coralliidae) from the islands and seamounts of the Hawaiian archipelago
Authors: BACO A. R. , CLARK A. M. , SHANK T. M. 
Published: January 2006

AbstractGorgonian octocorals are among the dominant deep‐sea benthic taxa of many seamounts. Seamount fauna are threatened by destructive fisheries practices, yet little is known about the physical and biological processes that maintain species on seamounts. Few informative molecular markers have been found in deep‐water corals or in gorgonian octocorals. Here we report the characterization of six highly polymorphic microsatellite loci for the deep‐sea precious coral Corallium lauuense from Hawaii using enriched genomic DNA libraries. These loci are being used to examine gene flow and stock structure among seamount and island populations to better understand dispersal and connectivity of seamount species.
Characterization of 35 microsatellite loci in the Pacific lion‐paw scallop (Nodipecten subnodosus) and their cross‐species amplification in four other scallops of the Pectinidae family
Authors: IBARRA ANA M. , PETERSEN JESSICA L. , FAMULA THOMAS R. , MAY BERNIE 
Published: January 2006

AbstractFour microsatellite‐enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion‐paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy–Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyse the population structure of the species in Mexico's Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty‐six primer pairs cross‐amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross‐amplified in the Argopecten species.
Characterization of 12 microsatellite markers in Serranus cabrilla (Pisces: Serranidae)
Authors: CARRERAS‐CARBONELL J. , MACPHERSON E. , PASCUAL M. 
Published: January 2006

AbstractThe commercial comber Serranus cabrilla is widely distributed in the Atlanto‐Mediterranean region, inhabiting a great variety of habitats and depths. We developed primers for 12 polymorphic microsatellite loci to analyse the genetic structure between comber populations and between their colour morphs in order to establish correct fisheries management. Characterization of 25 individuals from Columbretes Islands (Spain) showed an average large number of alleles (9.5 ± 1.3) and observed heterozygosity (0.657 ± 0.06). Only two loci showed significant departure from Hardy–Weinberg equilibrium. We found no evidence of linkage disequilibrium between pairs of loci. We rejected for primer design one clone with a microsatellite within the transposable element TX_FR2.
PRIMER NOTE. Using the incomplete genome of the ectomycorrhizal fungus Amanita bisporigera to identify molecular polymorphisms in the related Amanita phalloides
Authors: ADAMS RACHEL I. , HALLEN HEATHER E. , PRINGLE ANNE 
Published: January 2006

AbstractWe describe the cross‐genomic isolation of 13 single nucleotide polymorphisms (SNPs) and one variable microsatellite from five loci for the death cap mushroom Amanita phalloides. Microsatellite repeats were identified by searching the partial Amanita bisporigera genome. Flanking primers were designed for 25 of these microsatellite loci and tested for cross‐amplification in A. phalloides. One locus contained an interrupted, compound microsatellite, and four loci contained one to six SNPs. These results demonstrate the usefulness of even an incomplete genome to identify molecular markers for population studies in nonmodel organisms.
Genome‐wide assessment of tandem repeat markers for biogeographical analyses of the corn smut fungus, Ustilago maydis
Authors: MUNKACSI A. B. , KAWAKAMI S. , PAN J. J. , LEE K. , STOXEN S. , HANG J. , MAY G. 
Published: January 2006

AbstractIt has been postulated that the fungus Ustilago maydis followed its host maize around the world. In order to understand the biogeography of this fungus relative to its host, we used a bioinformatics approach to develop 86 tandem repeat markers useful for population studies of U. maydis. We characterized repeat motifs between two and 155 nucleotides using 36 isolates from USA, Mexico and South America. Our data suggested that (i) repeat motif length does not predict the number of alleles for that locus, and (ii) that the more polymorphic markers can resolve differences in a small geographical area.
Practical use of GPS‐localization of Feral PigeonsColumba liviain the urban environment
Authors: ROSE EVA , HAAG‐WACKERNAGEL DANIEL , NAGEL PETER 
Published: January 2006

Feral PigeonsColumba livialive in almost every city in the world and are often a problem because of their large numbers. Knowledge of the spatial use of the city by Pigeons is important for population control management. Previous studies have given contradictory results concerning the urban area used by Pigeons and their feeding strategies. We used the global positioning system (GPS) to investigate the spatial use of urban habitats by Feral Pigeons in Basel, Switzerland. The total ranges of the subpopulations varied between 32.9 and 306.3 ha and overlapped partially. The total ranges of individual Feral Pigeons varied between 2.9 and 150.6 ha. Pigeons from a single loft had one or two main feeding places and up to 33 other places that they used for occasional feeding or for resting. Individual Pigeons visited up to ten different locations. Our study shows that Feral Pigeons have individual feeding strategies and are flexible enough to adapt to different urban environments. Therefore, we must contradict the view that Feral Pigeons are dependent on intentional feeding by humans and are unable to fly more than a few hundred metres. Our results are important for Pigeon control management, biomonitoring projects using Feral Pigeons as indicators of pollution and the study of disease transmission. Pigeon control management based on killing has only a local and temporary effect, because Pigeon subpopulations are interconnected. Pigeons from other areas will replace removed individuals. Biomonitoring projects usually assume that Pigeons show a limited mobility. Our study reveals that this is not a generally valid assumption. Because Pigeon subpopulations are connected, diseases can be spread over an entire urban area. This is of human concern, as seven infectious diseases have been shown to be transmitted from Feral Pigeons to humans.