bluuebunny/crossref_metadata_2025_split huggingface dataset:
A type III polyketide synthase cluster in the phylum Planctomycetota is involved in alkylresorcinol biosynthesis
Authors: Milke Lars , Kabuu Moses , Zschoche Renè , Gätgens Jochem , Krumbach Karin , Carlstedt Kim-Loreen , Wurzbacher Carmen E. , Balluff Sven , Beemelmanns Christine , Jogler Christian , Marienhagen Jan , Kallscheuer Nicolai 
Published: February 2024

Abstract
Members of the bacterial phylum Planctomycetota have recently emerged as promising and for the most part untapped sources of novel bioactive compounds. The characterization of more than 100 novel species in the last decade stimulated recent bioprospection studies that start to unveil the chemical repertoire of the phylum. In this study, we performed systematic bioinformatic analyses based on the genomes of all 131 described members of the current phylum focusing on the identification of type III polyketide synthase (PKS) genes. Type III PKSs are versatile enzymes involved in the biosynthesis of a wide array of structurally diverse natural products with potent biological activities. We identified 96 putative type III PKS genes of which 58 are encoded in an operon with genes encoding a putative oxidoreductase and a methyltransferase. Sequence similarities on protein level and the genetic organization of the operon point towards a functional link to the structurally related hierridins recently discovered in picocyanobacteria. The heterologous expression of planctomycetal type III PKS genes from strains belonging to different families in an engineered Corynebacterium glutamicum strain led to the biosynthesis of pentadecyl- and heptadecylresorcinols. Phenotypic assays performed with the heterologous producer strains and a constructed type III PKS gene deletion mutant suggest that the natural function of the identified compounds differs from that confirmed in other bacterial alkylresorcinol producers.

Key points
• Planctomycetal type III polyketide synthases synthesize long-chain alkylresorcinols.
• Phylogenetic analyses suggest an ecological link to picocyanobacterial hierridins.
• Engineered C. glutamicum is suitable for an expression of planctomycete-derived genes.
Functional and radiological outcomes of periacetabular osteotomy for hip dysplasia in patients under fifty years using a minimally invasive approach—a single surgeon series with a minimum follow up of two years
Authors: Sunil Kumar Karadi Hari , Bhargava Kartik , Stamp Gregory , Malviya Ajay 
Published: February 2024

Abstract
Purpose
We conducted a retrospective analysis of prospectively collected data to evaluate (1) the extent of surgical correction following minimally invasive periacetabular osteotomy, (2) improvements in functional outcomes and any potential predictors for favourable outcome, and (3) complications after minimally invasive periacetabular osteotomy.

Methods
A total of 352 minimally invasive periacetabular osteotomy procedures were performed on 312 hip dysplasia patients between 2013 and 2020. Radiological parameters such as lateral centre edge angle, acetabular index, and Tönnis grade of arthritis were calculated. Patients also completed a range of patient reported outcome measures. Wilcoxon signed-rank tests were performed to assess for differences between patient reported outcome measures and radiological outcomes across the follow-up periods. Univariate linear regression and logistic regression were used to assess for predictors of change in functional outcome.

Results
Patients had a significant correction in mean lateral centre edge angle from 17.2° to 35.3° (p < 0.001) and mean acetabular index from 13.2° to − 0.82°. At one year follow-up all patient reported outcome measures were significantly greater than their baseline measurements and this improvement was maintained at two years. Changes in patient reported outcome measures were independent of radiological parameters such as change in the lateral centre edge angle and acetabular index, pre-operative Tönnis grade, and patient factors such as age and sex. A total of 5.11% of patients developed post-operative complications, with four requiring posterior column fixation. Four patients (1.12%) needed a total hip replacement.

Conclusion
Minimally invasive periacetabular osteotomy is a safe procedure that provides significant functional outcome improvements following surgery at six months which is maintained at two years. More than three-fourths of patients achieved improvement of iHOT-12 score beyond the minimal clinically important difference and more than half of the patients achieved substantial clinical benefit for iHOT-12 score.
Juvenile octocorals acquire similar algal symbiont assemblages across depths
Authors: Liberman Ronen , Voolstra Christian R. , Hume Benjamin C. C. , Benayahu Yehuda 
Published: February 2024

AbstractEstablishment of the coral–algal symbiosis begins during early ontogeny when juveniles acquire a mix of algae from their environment that often differs from the adults’ algal assemblages. Despite the importance of the type of Symbiodiniaceae to this symbiosis, it is largely unknown how coral host identity and environment affect symbiosis establishment and is affected by the genetic composition of the symbionts. Here, we reciprocally transplanted planulae of the octocoral Rhytisma fulvum (Forskål, 1775) across depths and monitored the algal assemblages in the developing juveniles for 11 months. We then compared these to adult assemblages using ITS2 metabarcoding. Juveniles were consistently dominated by Symbiodinium, in addition to multiple Cladocopium species, which shifted in dominance with the juvenile age but maintained high similarity across depths. The type of Symbiodiniaceae environmentally available thus likely contributes to the algal symbionts that are initially acquired, while host identity may play a significant role in selecting for symbionts that are maintained during juvenile development.
Optimal Rates of Approximation by Shallow ReLU$$^k$$ Neural Networks and Applications to Nonparametric Regression
Authors: Yang Yunfei , Zhou Ding-Xuan 
Published: February 2024

AbstractWe study the approximation capacity of some variation spaces corresponding to shallow ReLU$$^k$$

k

neural networks. It is shown that sufficiently smooth functions are contained in these spaces with finite variation norms. For functions with less smoothness, the approximation rates in terms of the variation norm are established. Using these results, we are able to prove the optimal approximation rates in terms of the number of neurons for shallow ReLU$$^k$$

k

neural networks. It is also shown how these results can be used to derive approximation bounds for deep neural networks and convolutional neural networks (CNNs). As applications, we study convergence rates for nonparametric regression using three ReLU neural network models: shallow neural network, over-parameterized neural network, and CNN. In particular, we show that shallow neural networks can achieve the minimax optimal rates for learning Hölder functions, which complements recent results for deep neural networks. It is also proven that over-parameterized (deep or shallow) neural networks can achieve nearly optimal rates for nonparametric regression.
Open airway surgery for post-COVID laryngotracheal stenosis
Authors: Nisa Lluís , Leroyer Hajdi , Sandu Kishore 
Published: February 2024

Abstract
Introduction
This study reports our experience with open reconstructive surgery in patients with laryngotracheal stenosis (LTS) following prolonged intubation and/or tracheostomy in the context of COVID-19.

Methods
All patients underwent a preoperative endoscopic airway assessment. Posterior glottic lesions were graded according to the Bogdasarian classification, subglottic-tracheal lesions according to the Cotton-Myers classification and postoperative complications reported by the Clavien–Dindo classification. We report postoperative outcomes and functional results in this patient subset.

Results
We include 14 patients diagnosed to have post COVID LTS, one female and 13 males. This group was compared with a control group, diagnosed with LTS following prolonged intubation. In the COVID group, mean age of patients at the time of the airway surgery was 52.1 ± 16.8 years (range: 13.7–76.3). More than half the patients were multi-morbid with hypertension and obesity being the most common conditions. Eleven patients had multi-site stenoses. Open surgical interventions performed were tracheal resection and anastomosis, laryngotracheal reconstruction and extended cricotracheal resection, and postoperative complications were seen in 12 (85.6%) patients. 70% patients with pre-existing tracheostomy were decannulated. Oral swallowing was not tolerated in one-fifth of the patients and a significant number of them have poor voice quality.

Conclusion
Post-COVID pandemic, airway surgeons are seeing an increased number of patients with complex LTS, and we report significant postoperative complications in this patient subset. Decannulation rates, voice and swallowing results are poor in patients with glottic involvement as compared to isolated tracheal stenosis.
Fluids and body composition during anesthesia in children and adolescents: A pilot study
Authors: Betti Céline , Busi Ilaria , Cortesi Cinzia , Anselmi Luciano , Mendoza-Sagaon Mario , Simonetti Giacomo D. 
Published: February 2024

AbstractThe purpose of this study is to evaluate the intracellular and extracellular volume before and after anesthesia in order to ascertain their variations and determine the potential utility of this information in optimizing intraoperative fluid administration practices. A bioimpedance spectroscopy device (body composition monitor, BCM) was used to measure total body fluid volume, extracellular volume, and intracellular volume. BCM measurements were performed before and after general anesthesia in unselected healthy children and adolescents visiting the Pediatric Institute of Southern Switzerland for low-risk surgical procedures hydrated with an isotonic solution. In 100 children and adolescents aged 7.0 (4.8–11) years (median and interquartile range), the average total body water increased perioperatively with a delta value of 182 (0–383) mL/m2 from pre- to postoperatively, as well as the extracellular water content, which had a similar increase with a delta value of 169 (19–307) mL/m2. The changes in total body water and extracellular water content significantly correlated with the amount of fluids administered. The intracellular water content did not significantly change. Conclusion: Intraoperative administration of isotonic solutions results in a significant fluid accumulation in low-risk schoolchildren during general anesthesia. The results suggest that children without major health problems undergoing short procedures do not need any perioperative intravenous fluid therapy, because they are allowed to take clear fluids up to 1 h prior anesthesia. In future studies, the use of BCM measurements has the potential to be valuable in guiding intraoperative fluid therapy.

What is Known:• Most children who undergo common surgical interventions or investigations requiring anesthesia are nowadays hydrated at a rate of 1700 mL/m2/day with an isotonic solution.• The use bioimpedance spectroscopy for the assessment of fluid status in healthy children has already been successfully validated.• The bioimpedance spectroscopy is already currently widely used in various nephrological settings to calculate fluid overload and determine patient’s optimal fluid status.

What is New:• Routine intraoperative fluid administration results in a significant fluid accumulation during general anesthesia in low-risk surgical procedures.• This observation might be relevant for children and adolescents with conditions predisposing to fluid retention.• In future studies, the use of BCM measurements has the potential to be valuable in guiding intraoperative fluid therapy.
Tissue distribution of cysteine string protein/DNAJC5 in C. elegans analysed by CRISPR/Cas9-mediated tagging of endogenous DNJ-14
Authors: Barker Eleanor , Morgan Alan , Barclay Jeff W. 
Published: February 2024

AbstractCysteine string protein (CSP) is a member of the DnaJ/Hsp40 family of molecular chaperones. CSP is enriched in neurons, where it mainly localises to synaptic vesicles. Mutations in CSP-encoding genes in flies, worms, mice and humans result in neuronal dysfunction, neurodegeneration and reduced lifespan. Most attention has therefore focused on CSP’s neuronal functions, although CSP is also expressed in non-neuronal cells. Here, we used genome editing to fluorescently tag the Caenorhabditis elegans CSP orthologue, dnj-14, to identify which tissues preferentially express CSP and hence may contribute to the observed mutant phenotypes. Replacement of dnj-14 with wrmScarlet caused a strong chemotaxis defect, as seen with other dnj-14 null mutants. In contrast, inserting the reporter in-frame to create a DNJ-14-wrmScarlet fusion protein had no effect on chemotaxis, indicating that C-terminal tagging does not impair DNJ-14 function. WrmScarlet fluorescence appeared most obvious in the intestine, head/pharynx, spermathecae and vulva/uterus in the reporter strains, suggesting that DNJ-14 is preferentially expressed in these tissues. Crossing the DNJ-14-wrmScarlet strain with GFP marker strains confirmed the intestinal and pharyngeal expression, but only a partial overlap with neuronal GFP was observed. DNJ-14-wrmScarlet fluorescence in the intestine was increased in response to starvation, which may be relevant to mammalian CSPα’s role in microautophagy. DNJ-14’s enrichment in worm reproductive tissues (spermathecae and vulva/uterus) parallels the testis-specific expression of CSPβ and CSPγ isoforms in mammals. Furthermore, CSPα messenger RNA is highly expressed in the human proximal digestive tract, suggesting that CSP may have a conserved, but overlooked, function within the gastrointestinal system.
Assessing patients’ needs in the follow-up after treatment for colorectal cancer—a mixed-method study
Authors: Voigt Kelly R. , de Bruijn Esmee A. , Wullaert Lissa , Witteveen Léon , Verhoef Cornelis , Husson Olga , Grünhagen Dirk J. 
Published: February 2024

Abstract
Purpose
The accessibility of cancer care faces challenges due to the rising prevalence of colorectal cancer (CRC) coupled with a shrinkage of healthcare professionals—known as the double aging phenomenon. To ensure sustainable and patient-centred care, innovative solutions are needed. This study aims to assess the needs of CRC patients regarding their follow-up care.

Methods
This study uses a mixed-method approach divided in three phases. The initial phase involved focus group sessions, followed by semi-structured interviews to identify patients’ needs during follow-up. Open analysis was done to define main themes and needs for patients. In the subsequent quantitative phase, a CRC follow-up needs questionnaire was distributed to patients in the follow-up.

Results
After two focus groups (n = 14) and interviews (n = 5), this study identified six main themes. Findings underscore the importance of providing assistance in managing both physical and mental challenges associated with cancer. Participants emphasised the need of a designated contact person and an increased focus on addressing psychological distress. Furthermore, patients desire individualised feedback on quality of life questionnaires, and obtaining tailored information. The subsequent questionnaire (n = 96) revealed the priority of different needs, with the highest priority being the need for simplified radiology results. A possible approach to address a part of the diverse needs could be the implementation of a platform; nearly 70% of patients expressed interest in the proposed platform.

Conclusions
CRC patients perceive substantial room for improvement of their follow-up care. Findings can help to develop a platform fulfilling the distinct demands of CRC patients during follow-up.
A Method for Reconstruction of Size Distributions from 3D Drone Image Analysis: A Case Study
Authors: Segarra Pablo , Sanchidrián José A. , Pötsch Markus , Iglesias Luis , Gómez Santiago , Gaich Andreas , Bernardini Maurizio 
Published: February 2024

AbstractThis paper describes a novel procedure to assess fragmentation from automatic analysis of 3D photogrammetric models with a commercial software. The muckpiles from 12 blasts were photographed with a conventional drone to build 3D photogrammetric models; the flights were made with a relatively constant ground sampling distance (GSD) of 6.2 sd 0.92 mm (mean and standard deviation, respectively). A comparison with already published mass-based size distributions from 11 of these blasts, shows a good performance of automatic 3D-fragmentation measurements in the coarse range (P ≥ 60%), while deviations between mass-based and 3D model fragmentation analysis grow towards the central-fines range. As a solution, the Swebrec function is fitted to the reliable part of the size distributions, well above the GSD, and then is extended towards the fines, down to a percentage passing of 5–10%. The suitable fitting range is obtained iteratively from the mass-based fragmentation data; the lower fragment size considered is independent of the model’s resolution (i.e. GSD) with mean of 357 mm (equivalent to a passing in the range 66–86%, and well above the GSD of our models). The resulting distributions match properly mass-based size distributions with relative errors in percentile sizes of 15.5 sd 3.4%, and they can be represented with the simplest form of the fragmentation-energy-fan. As a guideline for reconstruction of size distributions and fines assessment when mass-based data is not available, the lower-fitting limit of 357 mm yields reasonable results (mean errors in pass in the range 5–36%) for the present case. The errors are limited enough to keep a sound description of the variation of fragmentation with change in blast design.
Is it possible to extract primary maxillary molars without palatal injection?: a controlled clinical trial
Authors: TUĞUTLU Esra Ceren , SANCAK Kevser 
Published: February 2024

Abstract
Objectives
The absolute necessity of a palatal injection for the extraction of primary maxillary molars has never been explored, despite the fact that it is widely known that children do not tolerate local anesthetic injections into the palatal tissue well. The aim of this study was to compare separately the perception of pain in the absence of palatal injection after anesthesia and maxillary primary molar tooth extraction using different anesthetic solutions and different post-anesthetic waiting times.

Materials and methods
A single-blinded randomized controlled study was conducted in 78 participants (26 patients with palatal anesthesia (the control groups), and 26 patients with 5 min and 26 patients with 8 min post-anesthetic waiting time without palatal anesthesia (the study groups)). Subjective experiences of pain were evaluated separately after anesthesia and tooth extraction using the Visual Analog Scale (VAS) and the Wong-Baker Faces Pain Rating Scale (WBS).

Results
In terms of VAS scores obtained following administration of anesthesia, there was a statistically significant difference between the groups (p<0.05). VAS pain scores were reported to be lower in the groups without palatal anesthesia than in the groups with palatal anesthesia. No statistically significant difference was observed in VAS and Wong-Baker scores after tooth extraction between the groups with and without palatal anesthesia (P>0.05).

Conclusions
While the pain reported following administration of anesthesia was found to be higher in the groups receiving palatal anesthesia, no difference was found between the groups in the pain reported after tooth extraction.

Clinical relevance
Extraction of maxillary primary molars is possible without palatal injection by injecting 4% articaine or 2% lidocaine into the buccal vestibule of the tooth with a waiting time of 5 or 8 min.
Analysis of retromuscular drain output and postoperative outcomes for heavyweight versus mediumweight polypropylene mesh following open ventral hernia repair
Authors: Essani V. , Maskal S. M. , Ellis R. C. , Messer N. , Tu C. , Miller B. T. , Petro C. C. , Beffa L. R. A. , Krpata D. M. , Prabhu A. S. , Rosen M. J. 
Published: February 2024

Abstract
Purpose
Heavyweight polypropylene (HWPP) mesh is thought to increase inflammatory response and delay tissue integration compared to mediumweight (MWPP). Reactive fluid volume (i.e., drain output) may be a reasonable surrogate for integration. We hypothesized that daily drain output is higher with HWPP compared to MWPP in open retromuscular ventral hernia repair (VHR).

Methods
This is a post-hoc analysis of a multicenter, randomized clinical trial conducted March 2017–April 2019 comparing MWPP and HWPP for VHR. Retromuscular drain output in milliliters was measured at 24-h intervals up to postoperative day seven. Univariate analyses compared differences in daily drain output and time to drain removal. Multivariable analyses compared total drain output and wound morbidity within 30 days and hernia recurrence at 1 year.

Results
288 patients were included; 140 (48.6%) HWPP and 148 (51.4%) MWPP. Daily drain output for days 1–3 was higher for HWPP vs. MWPP (total volume: 837.8 mL vs. 656.5 mL) (p < 0.001), but similar on days 4–7 (p > 0.05). Median drain removal time was 5 days for both groups. Total drain output was not predictive of 30-day wound morbidity (p > 0.05) or hernia recurrence at 1 year (OR 1, p = 0.29).

Conclusion
While HWPP mesh initially had higher drain outputs, it rapidly returned to levels similar to MWPP by postoperative day three and there was no difference in clinical outcomes. We believe that drains placed around HWPP mesh can be managed similarly to MWPP mesh.
Individuals with a ventral hernia who report moderate to high fear have worse functional performance than those with low fear
Authors: Arhos Elanna K. , Poulose Benjamin K. , Di Stasi Stephanie , Chaudhari Ajit M. W. 
Published: February 2024

Abstract
Purpose
Ventral hernia repairs (VHR) are performed to restore the integrity of the abdominal wall. Fear of movement, or kinesiophobia, may develop in patients with ventral hernia due to pain and functional impairments, however it has not yet been objectively measured in this patient population. The purpose of this study was to test the hypothesis that in patients with ventral hernia awaiting surgical repair, higher levels of kinesiophobia would be associated with poorer mobility, abdominal core function, and quality of life.

Methods
Seventy-seven participants scheduled for ventral hernia repair were enrolled as part of an ongoing randomized controlled trial (NCT05142618). The Tampa Scale of Kinesiophobia (TSK-11) is an 11-item questionnaire that asks about fear of movement and physical activity restriction. Participants were split into groups based on their TSK-11 score (minimal, low, moderate to high). Primary outcome measures included the five-time sit-to-stand (5xSTS), Quiet Unstable Sitting Test (QUeST), and the Hernia-Related Quality-of-Life (HerQLeS) survey. A one-way ANOVA with a Bonferroni correction compared QUeST, 5xSTS, and HerQLes results between groups.

Results
Groups were significantly different on 5xSTS (minimal: 11.4 ± 2.6 s, low: 13.8 ± 3.1 s, moderate to high: 17.8 ± 9.8 s; p = 0.001) and HerQLes (minimal: 58.0 ± 27.8, low: 49.4 ± 22.0, moderate to high: 30.6 ± 25.3; p = 0.003) but not QUeST (minimal: − 2.8 ± 2.5, low: − 6.8 ± 10.0, moderate to high: − 5.5 ± 5.0; p = 0.16).

Conclusion
Individuals with moderate to high kinesiophobia have worse pre-operative performance-based (5xSTS) and self-reported (HerQLes) function and quality of life than those with minimal and low kinesiophobia. Future research should examine the influence of kinesiophobia on post-operative outcomes as it may be a potent target for rehabilitation.