bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and application of a time-saving mode in China for the treatment of acute ischemic stroke
Authors: Yan Yazhou , Du Li , Shangguan Xiu , Li Lujun , Chi Yuxiang , Wang Yu , Cheng Shuai , Huang Qinghai , Pan Yuan , Xin Tao 
Published: March 2024

ObjectiveTo explore the construction and application in the practice of green channel in No. 971 Naval Hospital of PLA (No. 971 Hospital mode) for the treatment of acute ischemic stroke (AIS).MethodsThis retrospective study involved a cohort of 694 suspected stroke patients from December 2022 to November 2023 undergoing emergency treatment for stroke at our institution. Among them, 483 patients were treated with standard green channel (the control group), and 211 patients adopted the No. 971 Hospital mode for treatment (the study group). The biggest difference between the two groups was that the treatment process started before admission. We compared the effectiveness of the emergency treatment between the two groups and the thrombolysis treatment.ResultsCompared with control group, the accuracy rate of determining stroke and the rate of thrombolysis were significantly higher (p = 0.002, 0.039) and the door to doctor arrival time (DAT) and the door to CT scan time (DCT) of the study group was significantly shorter (all p &lt; 0.001). There were 49 patients (10.1%) and 33 patients (15.6%) from the control group and study group receiving thrombolysis, respectively. The DAT, DCT, imaging to needle time (INT), and door to needle time (DNT) of patients receiving thrombolysis in the study group were significantly shorter than that in the control group (all p &lt; 0.01). The NIHSS in the study group after the thrombolysis was lower than that in the control group (p = 0.042).ConclusionNo. 971 Hospital model can effectively shorten DAT, DCT, INT, and DNT, and improve the effectiveness of thrombolysis and prognoses of AIS patients.
Association between amino acids and recent osteoporotic fracture: a matched incident case-control study
Authors: Liang Bing , Shi Xinyan , Wang Xinwei , Ma Chao , Leslie William D. , Lix Lisa M. , Shi Xianbao , Kan Bo , Yang Shuman 
Published: March 2024

ContextOsteoporotic fracture is a major public health issue globally. Human research on the association between amino acids (AAs) and fracture is still lacking.ObjectiveTo examine the association between AAs and recent osteoporotic fractures.MethodsThis age and sex matched incident case-control study identified 44 recent x-ray confirmed fracture cases in the Second Hospital of Jilin University and 88 community-based healthy controls aged 50+ years. Plasma AAs were measured by high performance liquid chromatography coupled with mass spectrometry. After adjusting for covariates (i.e., body mass index, milk intake &gt;1 time/week, falls and physical activity), we conducted conditional logistical regression models to test the association between AAs and fracture.ResultsAmong cases there were 23 (52.3%) hip fractures and 21 (47.7%) non-hip fractures. Total, essential, and non-essential AAs were significantly lower in cases than in controls. In the multivariable conditional logistic regression models, after adjusting for covariates, each standard deviation increase in the total (odds ratio [OR]: 0.304; 95% confidence interval [CI]: 0.117–0.794), essential (OR: 0.408; 95% CI: 0.181–0.923) and non-essential AAs (OR: 0.290; 95%CI: 0.107–0.782) was negatively associated with recent fracture. These inverse associations were mainly found for hip fracture, rather than non-hip fractures. Among these AAs, lysine, alanine, arginine, glutamine, histidine and piperamide showed the significantly negative associations with fracture.ConclusionThere was a negative relationship between AAs and recent osteoporotic fracture; such relationship appeared to be more obvious for hip fracture.
Plasma metabolomics reveals risk factors for lung adenocarcinoma
Authors: Yu Mengjie , Wen Wei , Wang Yue , Shan Xia , Yi Xin , Zhu Wei , Aa Jiye , Wang Guangji 
Published: March 2024

BackgroundMetabolic reprogramming plays a significant role in the advancement of lung adenocarcinoma (LUAD), yet the precise metabolic changes remain incompletely understood. This study aims to uncover metabolic indicators associated with the progression of LUAD.MethodsA total of 1083 subjects were recruited, including 670 LUAD, 135 benign lung nodules (BLN) and 278 healthy controls (HC). Gas chromatography-mass spectrometry (GC/MS) was used to identify and quantify plasma metabolites. Odds ratios (ORs) were calculated to determine LUAD risk factors, and machine learning algorithms were utilized to differentiate LUAD from BLN.ResultsHigh levels of oxalate, glycolate, glycine, glyceric acid, aminomalonic acid, and creatinine were identified as risk factors for LUAD (adjusted ORs&gt;1.2, P&lt;0.03). Remarkably, oxalate emerged as a distinctive metabolic risk factor exhibiting a strong correlation with the progression of LUAD (adjusted OR=5.107, P&lt;0.001; advanced-stage vs. early-stage). The Random Forest (RF) model demonstrated a high degree of efficacy in distinguishing between LUAD and BLN (accuracy = 1.00 and 0.73, F1-score= 1.00 and 0.79, and AUC = 1.00 and 0.76 in the training and validation sets, respectively). TCGA and GTEx gene expression data have shown that lactate dehydrogenase A (LDHA), a crucial enzyme involved in oxalate metabolism, is increasingly expressed in the progression of LUAD. High LDHA expression levels in LUAD patients are also linked to poor prognoses (HR=1.66, 95% CI=1.34-2.07, P&lt;0.001).ConclusionsThis study reveals risk factors associated with LUAD.
Anti-angiogenic therapy or immunotherapy? A real-world study of patients with advanced non-small cell lung cancer with EGFR/HER2 exon 20 insertion mutations
Authors: Li Jiaqi , Xie Mengqing , Zhao Ruiying , Qiang Huiping , Chang Qing , Qian Jialin , Lu Haijiao , Shen Yinchen , Han Yuchen , Su Chunxia , Chu Tianqing 
Published: March 2024

BackgroundFor patients with EGFR/HER2 exon20 insertions, platinum-containing double-drug chemotherapy is still the standard treatment method. First-generation TKIs have almost no therapeutic activity against EGFR exon 20 insertions. The efficacy of second-and third-generation TKIs is still controversial. Immunotherapy research is scarce, and there is an urgent need for more evidence and new treatment options for this group of patients.MethodsWe reviewed patients with advanced NSCLC with EGFR/HER2 exon 20 insertion mutations treated in Shanghai Chest Hospital and Shanghai Pulmonary Hospital from 2015 to 2022 and assessed the efficacy of receiving chemotherapy, anti-angiogenic therapy and immunotherapy, including objective response rate (ORR) and disease control rate (DCR), and compared progression-free survival (PFS) and overall survival (OS).ResultsOf the 126 patients included in the study, 51 patients had EGFR20ins mutations and 7 5 patients had HER2-20ins mutations. In the first-line treatment, bevacizumab + chemotherapy (Beva+Chemo), ICI+chemotherapy (ICI+Chemo), compared with chemotherapy alone (Chemo), ORR: 40% vs 33.3% vs 15% (p=0.0168); DCR: 84% vs 80.9% vs 67.5% (p=0.1817); median PFS: 8.3 vs 7.0 vs 4.6 months (p=0.0032), ICI+Chemo has a trend of benefiting on OS. Stratified analysis showed that compared with chemotherapy, ICI+Chemo was more effective for EGFR20ins mutation with median PFS: 10.3 vs. 6.3m (P=0.013); Beva+Chemo was more effective for HER2-20ins mutation, with a median PFS: 6.6 vs. 4.3m (p=0.030). In the second-line treatment of EGFR20ins mutation, bevacizumab + chemotherapy has a significant advantage in PFS compared with targeted therapy, median PFS:10.8 vs 4.0 months (P=0.016).ConclusionFor patients with EGFR20ins mutation, compared to chemotherapy, ICI+Chemo prolongs PFS, and after chemotherapy progression, bevacizumab combined with chemotherapy seems better than Furmonertinib-based targeted therapy on PFS. For HER2-20ins mutation, Beva+Chemo may be a better choice.
Clinical and radiomics integrated nomogram for preoperative prediction of tumor-infiltrating lymphocytes in patients with triple-negative breast cancer
Authors: Hu Ling , Jin Peile , Xu Wen , Wang Chao , Huang Pintong 
Published: March 2024

ObjectivesThe present study aimed to develop a radiomics nomogram based on conventional ultrasound (CUS) to preoperatively distinguish high tumor-infiltrating lymphocytes (TILs) and low TILs in triple-negative breast cancer (TNBC) patients.MethodsIn the present study, 145 TNBC patients were retrospectively included. Pathological evaluation of TILs in the hematoxylin and eosin sections was set as the gold standard. The patients were randomly allocated into training dataset and validation dataset with a ratio of 7:3. Clinical features (age and CUS features) and radiomics features were collected. Then, the Rad-score model was constructed after the radiomics feature selection. The clinical features model and clinical features plus Rad-score (Clin+RS) model were built using logistic regression analysis. Furthermore, the performance of the models was evaluated by analyzing the receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA).ResultsUnivariate analysis and LASSO regression were employed to identify a subset of 25 radiomics features from a pool of 837 radiomics features, followed by the calculation of Rad-score. The Clin+RS integrated model, which combined posterior echo and Rad-score, demonstrated better predictive performance compared to both the Rad-score model and clinical model, achieving AUC values of 0.848 in the training dataset and 0.847 in the validation dataset.ConclusionThe Clin+RS integrated model, incorporating posterior echo and Rad-score, demonstrated an acceptable preoperative evaluation of the TIL level. The Clin+RS integrated nomogram holds tremendous potential for preoperative individualized prediction of the TIL level in TNBC.
Eosinophilic esophagitis improved by the discontinuation of sublingual immunotherapy for aeroallergens: a case report
Authors: Monzani Alice , Cerutti Marta , Curto Sara , Lovaste Sara , Coppola Marta , Mercalli Francesca , Saettone Silvia , Rabbone Ivana 
Published: March 2024

IntroductionEosinophilic esophagitis (EoE) is a chronic, immune-mediated inflammation of the esophagus, characterized by symptoms related to esophageal dysfunction, resulting from severe eosinophilic infiltration of the esophageal mucosa. It is common in atopic subjects and food antigens have been identified as the most common triggers. However, a seasonal variation in EoE prevalence, correlated with air pollen levels, is reported, suggesting that also aeroallergens may play a role. Little is known about the interplay between EoE and concomitant atopy treatment for aeroallergens.Case presentationWe describe the case of an 11-year-old boy who presented dysphagia, vomiting, drooling, and chest pain while eating meat, developed 15 months after receiving sublingual immunotherapy (SLIT) for Alternaria (SUBLIVAC®). He underwent esophagogastroduodenoscopy (EGD) revealing severe eosinophilic predominant inflammation (100 eos/HPF), consistent with the diagnosis of EoE, not improving at the EGDs performed after both omeprazole and topical corticosteroids treatment, despite symptom improvement. Afterward, immunotherapy was switched from sublingual to injective form. At the EGD performed 1 month later, macroscopic examination of the esophageal mucosa was normal and eosinophilic infiltration was significantly decreased (5–10 eos/HPF).ConclusionsSLIT may induce EoE by chronic antigenic exposure of oral mucosa in patients with a robust allergic susceptibility: while attenuating the IgE-mediated immune reactions, the progressive contact with the causative allergen might induce a chronic stimulation of the immune system with the consequent activation of tissue eosinophils. Our data suggest monitoring patients receiving SLIT for EoE symptoms and to discontinue SLIT on their earlier appearance, possibly as a first-line treatment.
Population genetic variation and geographic distribution of suitable areas of Coptis species in China
Authors: Chi Yujie , Liu Changli , Liu Wei , Tian Xufang , Hu Juan , Wang Bo , Liu Di , Liu Yifei 
Published: March 2024

IntroductionThe rhizomes of Coptis plants have been used in traditional Chinese medicine over 2000 years. Due to increasing market demand, the overexploitation of wild populations, habitat degradation and indiscriminate artificial cultivation of Coptis species have severely damaged the native germplasms of species in China.MethodsGenome-wide simple-sequence repeat (SSR) markers were developed using the genomic data of C. chinensis. Population genetic diversity and structure of 345 Coptis accessions collected from 32 different populations were performed based on these SSRs. The distribution of suitable areas for three taxa in China was predicted and the effects of environmental variables on genetic diversity in relation to different population distributions were further analyzed.Results22 primer pairs were selected as clear, stable, and polymorphic SSR markers. These had an average of 16.41 alleles and an average polymorphism information content (PIC) value of 0.664. In the neighbor-joining (N-J) clustering analysis, the 345 individuals clustered into three groups, with C. chinensis, C. chinensis var. brevisepala and C. teeta being clearly separated. All C. chinensis accessions were further divided into four subgroups in the population structure analysis. The predicted distributions of suitable areas and the environmental variables shaping these distributions varied considerably among the three species.DiscussionOverall, the amount of solar radiation, precipitation and altitude were the most important environmental variables influencing the distribution and genetic variation of three species. The findings will provide key information to guide the conservation of genetic resources and construction of a core reserve for species.
Response of underwater photosynthesis to light, CO2, temperature, and submergence time of Taxodium distichum, a flood-tolerant tree
Authors: Guo Jinbo , Xue Jianhui , Yin Yunlong , Pedersen Ole , Hua Jianfeng 
Published: March 2024

IntroductionPartial or complete submergence of trees can occur in natural wetlands during times of high waters, but the submergence events have increased in severity and frequency over the past decades. Taxodium distichum is well-known for its waterlogging tolerance, but there are also numerous observations of this species becoming partially or complete submerged for longer periods of time. Consequently, the aims of the present study were to characterize underwater net photosynthesis (PN) and leaf anatomy of T. distichum with time of submergence.MethodsWe completely submerged 6 months old seedling of T. distichum and diagnosed underwater (PN), hydrophobicity, gas film thickness, Chlorophyll concentration and needles anatomy at discrete time points during a 30-day submergence event. We also constructed response curves of underwater PN to CO2, light and temperature.ResultsDuring the 30-day submergence period, no growth or formation new leaves were observed, and therefore T. distichum shows a quiescence response to submergence. The hydrophobicity of the needles declined during the submergence event resulting in complete loss of gas films. However, the Chlorophyll concentration of the needles also declined significantly, and it was there not possible to identify the main cause of the corresponding significant decline in underwater PN. Nevertheless, even after 30 days of complete submergence, the needles still retained some capacity for underwater photosynthesis under optimal light and CO2 conditions.DiscussionHowever, to fully understand the stunning submergence tolerance of T. distichum, we propose that future research concentrate on unravelling the finer details in needle anatomy and biochemistry as these changes occur during submergence.
The interpersonal benefits of goal adjustment capacities: the sample case of coping with poor sleep in couples
Authors: Barlow Meaghan A. , Wrosch Carsten , Hoppmann Christiane A. 
Published: March 2024

IntroductionThis study examined the role of goal adjustment capacities and coping in the association between spousal sleep efficiency and relationship satisfaction in romantic couples.MethodA community lifespan sample of 113 heterosexual couples (age range = 21–82 years) was recruited using newspaper advertisements in the Greater Montreal Area from June 2011 to December 2012. Participants completed study measures (i.e., Goal Adjustment Scale, Brief Pittsburgh Sleep Quality Index, Relationship Assessment Scale, and the Brief Cope) at two time points, ~1 year apart.ResultsThe results of actor-partner interdependence models with moderation (MIXED procedure in SPSS) reveal that goal disengagement buffered people from worsening relationship satisfaction associated with poor spousal sleep [95% CI B (−1.17, −0.12)], in part via increases in actor active coping [95% CI B (−0.32, −0.02)] and decreases in partner self-blame [95% CI B (−0.28, −0.01)]. Goal reengagement was related to diminished relationship satisfaction in response to poor own sleep [95% CI B (0.59, 1.79)], in part through increases in actor behavioral disengagement [95% CI B (0.05, 0.41)].DiscussionThese findings point to a need for future studies to examine goal adjustment capacities and relationship-specific coping strategies as potential targets of intervention to maintain peoples' relationship satisfaction in the face of sleep problems.
The impact of non-stereotypical gender role endorsement in live broadcasting on consumers’ purchase intention
Authors: Fu Jia , Huang Simin , Chen Xiaolin 
Published: March 2024

IntroductionNon-stereotypical gender role endorsement is becoming more common in e-commerce live broadcasting. However, there is relatively little research on this topic, and the mechanism of its impact on purchase intention is not yet clear. Based on schema theory and experimental methods, this study explores the impact of non-stereotypical gender role endorsement (compared to stereotypical gender role endorsement) on purchase intention in e-commerce live broadcasting. Besides, we take traditional gender ideology as the moderating variable.MethodsWe first selected experimental materials available for formal experiments through two pre-experiments. Secondly, this study conducted experiments on male/female product groups, respectively. Participants were recruited through the Credamo platform for both experiments.ResultsExperiment 1 indicates that for female product, stereotypical gender role endorsement triggers higher consumer purchase intention compared to non-stereotypical gender role endorsement. The subsequent moderating effect test results manifest that traditional gender ideology plays a moderating role in this effect. Experiment 2 shows that for male product, there is no significant difference in the impact of the two types of endorsement on consumers’ purchase intention. In other words, non-stereotypical gender role endorsement does affect consumers’ purchase intention, but this effect exists only in female product, and is more significant for consumers with a high level of traditional gender ideology.DiscussionThis study not only has certain theoretical significance for expanding the application boundaries of schema theory and congruence between celebrities and products endorsed, but also has practical significance for brand owners and streamers to effectively adopt non-stereotypical gender role endorsement to enhance purchase intention.
Patterns and profiles of drug resistance-conferring mutations in Mycobacterium tuberculosis genotypes isolated from tuberculosis-suspected attendees of spiritual holy water sites in Northwest Ethiopia
Authors: Reta Melese Abate , Maningi Nontuthuko Excellent , Fourie P. Bernard 
Published: March 2024

PurposeThis study examined the patterns and frequency of genetic changes responsible for resistance to first-line (rifampicin and isoniazid), fluoroquinolones, and second-line injectable drugs in drug-resistant Mycobacterium tuberculosis (MTB) isolated from culture-positive pulmonary tuberculosis (PTB) symptomatic attendees of spiritual holy water sites (HWSs) in the Amhara region.Patients and methodsFrom June 2019 to March 2020, a cross-sectional study was carried out. A total of 122 culture-positive MTB isolates from PTB-suspected attendees of HWSs in the Amhara region were evaluated for their drug resistance profiles, and characterized gene mutations conferring resistance to rifampicin (RIF), isoniazid (INH), fluoroquinolones (FLQs), and second-line injectable drugs (SLIDs) using GenoType®MTBDRplus VER2.0 and GenoType®MTBDRsl VER2.0. Drug-resistant MTB isolates were Spoligotyped following the manufacturer’s protocol.ResultsGenetic changes (mutations) responsible for resistance to RIF, INH, and FLQs were identified in 15/122 (12.3%), 20/122 (16.4%), and 5/20 (25%) of MTB isolates, respectively. In RIF-resistant, rpoB/Ser531Lue (n = 12, 80%) was most frequent followed by His526Tyr (6.7%). Amongst INH-resistant isolates, katG/Ser315Thr1 (n = 19, 95%) was the most frequent. Of 15 MDR-TB, the majority (n = 12, 80%) isolates had mutations at both rpoB/Ser531Leu and katG/Ser315Thr1. All 20 INH and/or RIF-resistant isolates were tested with the MTBDRsl VER 2.0, yielding 5 FLQs-resistant isolates with gene mutations at rpoB/Ser531Lue, katG/Ser315Thr1, and gyrA/Asp94Ala genes. Of 20 Spoligotyped drug-resistant MTB isolates, the majority (n = 11, 55%) and 6 (30%) were SIT149/T3-ETH and SIT21/CAS1-Kili sublineages, respectively; and they were any INH-resistant (mono-hetero/multi-). Of 15 RIF-resistant (RR/MDR-TB) isolates, 7 were SIT149/T3-ETH, while 6 were SIT21/CAS1-Kili sublineages. FLQ resistance was detected in four SIT21/CAS1-Kili lineages.ConclusionIn the current study, the most common gene mutations responsible for resistance to INH, RIF, and FLQs were identified. SIT149/T3-ETH and SIT21/CAS1-Kili constitute the majority of drug-resistant TB (DR-TB) isolates. To further understand the complete spectrum of genetic changes/mutations and related genotypes, a sequencing technology is warranted.
Parents, preschoolers, and napping: the development and psychometric properties of two Nap Belief Scales in two independent samples
Authors: Newton Adam T. , Reid Graham J. 
Published: March 2024

IntroductionMost children cease napping between 2 and 5 years old. Little is known about the predictors of this cessation. Parents' sleep-related beliefs aid in understanding children's nighttime sleep behaviors, but few index daytime sleep beliefs.MethodsTwo measures of parents' napping beliefs were developed and evaluated-the Parents' Nap Beliefs Scale (14 items) and the Reasons Children Nap Scale (19 items). Canadian parents of 1–5-year-old children completed these questionnaires and other sleep-related measures in independent pilot (n = 201) and replication (n = 702) samples. In the replication sample, a subsample of parents also completed 1–3 weeks of daily sleep diaries. The samples were representative of the Canadian population by ethnicity and region.ResultsIn both samples, both measures demonstrated strong construct validity, convergent and divergent validity, and internal consistency. The Parents' Nap Beliefs Scale was composed of two factors: (a) Positive Beliefs and (b) Negative Beliefs about napping. The Reasons Children Nap Scale was composed of two higher order factors and five lower order factors: (a) Encouragement Reasons (Child related; Parent related) and (b) Discouragement Reasons (Child prefers not to nap; Child functions well without a nap; Scheduling).DiscussionFuture research should (a) test these scales as longitudinal determinants of children's nap behavior and cessation, (b) evaluate parental Nap Beliefs in non-Western cultures, and (c) adapt these scales for use with childcare providers.