bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global, Regional, and National Disease Burden and Prediction Analysis of Colorectal Cancer Attributable to Tobacco, Alcohol, and Obesity From 1990 to 2030
Authors: Deng Yuqi , Wang Yajie , Yang Jinsai , Luo Xinyu , Qiu Jieya , Long Rou , Zhang Chaohui , Li Jiale , Tang Guiyang , Chen Lili , Zuo Jianhong 
Published: February 2025

BackgroundColorectal cancer (CRC) ranks among the highest in incidence and mortality rates globally. A significant portion of Colorectal cancer cases and deaths can be attributed to modifiable risk factors, with smoking, alcohol use, and high body mass index (BMI) being the three most prominent. However, the impact of these risk factors on Colorectal cancer across regions, genders, and age groups remains insufficiently characterized.MethodsUtilizing data from the Global Burden of Disease (GBD) study 2019, restrictive cubic splines (RCS) and quantile regression analyses are applied to explore the relationship between the Socio-Demographic Index (SDI) and ASMR or ASDR. Additionally, gender differences, changes across different SDI levels, and age group trends in smoking, alcohol use, and high BMI over the 30-year period are analyzed. The Bayesian age-period-cohort (BAPC) model is employed to predict mortality trends from 2020 to 2030, aiming to explore the epidemiological and sociodemographic transitions in the Colorectal cancer disease burden attributed to smoking, alcohol use, and high BMI.ResultsIn 2019, the number of colorectal cancer deaths globally attributable to risk factors as smoking, alcohol consumption, and obesity increased to 142,931, 52,495, and 85,882 cases respectively, collectively accounting for approximately one-third of all Colorectal cancer-related deaths. Notably, there is an upward trend in early-onset Colorectal cancer mortality associated with these factors.DiscussionTo reduce the burden of Colorectal cancer, it is recommended to enhance health education, promote smoking cessation and alcohol moderation, and increase the coverage and participation in Colorectal cancer screening, which are crucial for lowering Colorectal cancer mortality rates. These findings are vital for the development of public health policies and intervention measures to reduce the global disease burden. They provide guidance for Colorectal cancer prevention across different regions, genders, and age groups worldwide.
Gentiopicroside targeting AKT1 activates HIF-1α/VEGF axis promoting diabetic ulcer wound healing
Authors: Wang Xinxia , Liu Mingyan , Wu Yao , Sun Jianguo , Liu Li , Pan Zheng 
Published: February 2025

BackgoundGentiopicroside (GSP) have been proven to accelerate the healing of diabetic ulcers (DU), but the underlying molecular mechanisms remain unclear. This study aims to explore the mechanism by which GSP accelerates the healing of DU.MethodThe targets of GSP were firstly predicted using the SuperPred, SwissTargetPrediction, and Pharmmapper databases; DU-related transcriptome data were obtained from the GEO database, including GSE147890, GSE68183, and GSE199939; differential expression analysis was conducted using the Limma package, and DU-related targets were identified after summarization and de-duplication. Then, Potential targets for GSP treatment of DU were screened by Venn analysis; core targets for GSP treatment of DU were selected by constructing a protein-protein interaction (PPI) network; the mechanism of GSP treatment of DU was predicted by GO and KEGG enrichment analysis. Finally, the target binding of GSP to core targets was evaluated by molecular docking and CETSA assay, and in vitro experiments were conducted using L929 cells to validate the findings.ResultA total of 538 targets of GSP and 10795 DU-related targets were predicted; Venn analysis identified 215 potential targets for GSP to accelerate DU wound healing; PPI network analysis suggested that AKT1 may be core targets for GSP treatment of DU; GO and KEGG enrichment analysis showed that pathways such as HIF-1 and VEGF are closely related to the treatment of DU with GSP, and it also participates in the regulation of various biological processes such as small molecule catabolism and leukocyte migration to exert its therapeutic effect on DU. Molecular docking and CETSA detection indicated that GSP can target bind to AKT1. The experimental results confirmed that GSP can significantly promote the proliferation and migration of L929 cells. Westen Blot results showed that GSP can accelerate DU wound healing via AKT1/HIF-1α/VEGF axis.ConclusionGSP target binding to AKT1 accelerates DU wound healing via the regulation of HIF-1α/VEGF axis.
Acetate and propionate vs. iTBS as a novel method for cognitive dysfunction and anxiety symptoms in delayed encephalopathy after acute carbon monoxide poisoning rat
Authors: Meng Tianyu , Zhang Xin , Zhao Jili , Xue Hui , Yu Lehua 
Published: February 2025

BackgroundThe optimal treatment methods for delayed encephalopathy after acute carbon monoxide (CO) poisoning (DEACMP) were not identified. Thus, this study was conducted to compare the efficacies of intermittent theta burst stimulation (iTBS) and short-chain fatty acids (SCFAs) in treating cognitive dysfunction and anxiety symptoms of DEACMP rat.MethodsIn phase I, a DEACMP rat model was built to assess the inflammation levels in the hippocampus and levels of SCFAs in the serum of DEACMP rats. In phase II, DEACMP rats were randomly assigned into four groups: DEACMP + placebo, DEACMP + SCFAs, DEACMP + sham iTBS, and DEACMP + iTBS. The intervention was continued for 2 weeks. A Morris water maze and open field tests were used to assess cognitive function and anxiety symptoms, respectively.ResultsThe levels of three inflammatory factors (IL-1β, IL-6, and TNF-α) and two SCFAs (acetate and propionate) were significantly increased and decreased, respectively, in DEACMP rats. After treatment, cognitive dysfunction and anxiety symptoms were significantly improved in the DEACMP + iTBS group and the DEACMP + SCFAs (consisting of acetate and propionate) group. Both SCFAs and iTBS could significantly improve the increased levels of IL-1β, IL-6, and TNF-α in the hippocampus, and SCFAs could also improve the decreased levels of GPR41, GPR43, dopamine, and norepinephrine in the hippocampus of DEACMP rats.ConclusionThese results indicate that both iTBS and SCFA solutions consisting of acetate and propionate produced good effects on DEACMP rats by regulating inflammation levels in the hippocampus, and acetate/propionate–GPR41/GPR43–IL-1β/IL-6/TNF-α–dopamine/norepinephrine may be a potential pathway in SCFAs for the treatment of DEACMP.
Myositis of pterygoid muscles and superior ophthalmic vein and cavernous sinus thrombosis in active Crohn’s disease undergoing ustekinumab treatment: a case report and literature review
Authors: Winters Robbe , Kaut Sara , Govaerts Dries , De Poortere Aaron , Mombaerts Ilse , Hoffman Ilse , Willaert Robin , van Hoeve Karen 
Published: February 2025

IntroductionMyositis is a rare extra-intestinal presentation in patients with inflammatory bowel diseases (IBD), and its occurrence has only been described in a few case reports. However, it is essential to consider other potential causes as patients with IBD are more susceptible to infections due to their immunocompromised status, which may also be exacerbated by immunosuppressive drugs contributing to myositis. Our case highlights the complexity and challenges in diagnosing and managing myositis in patients with IBD as well as a review of the literature.Case reportWe report the case of a 17-year-old girl with active Crohn’s disease (CD) undergoing ustekinumab (UST) treatment. She presented with sudden onset of pain and rapid progressive swelling of both jaws, along with eyelid swelling, blurred vision, and diplopia. Radiologic imaging revealed myositis affecting the pterygoid muscles, combined with thrombosis of the left superior ophthalmic vein and (partially) of the left cavernous sinus. Symptoms resolved completely after stopping UST treatment and initiating corticosteroids and enoxaparine.DiscussionOur report accounts for the second patient described in literature with myositis associated with CD while being treated with UST. The causal factor remains speculative, as both active CD and UST treatment may contribute to this complication. Sharing clinical experiences and reporting possible adverse events to regulatory agencies can enhance our understanding of rare complications and improve patient outcomes by providing therapeutic strategies.
Microbiota and metabolomic profiling coupled with machine learning to identify biomarkers and drug targets in nasopharyngeal carcinoma
Authors: Liu Junsong , Xu Chongwen , Wang Rui , Huang Jianhua , Zhao Ruimin , Wang Rui 
Published: February 2025

BackgroundNasopharyngeal carcinoma (NPC) is a prevalent malignancy in certain regions, with radiotherapy as the standard treatment. However, resistance to radiotherapy remains a critical challenge, necessitating the identification of novel biomarkers and therapeutic targets. The tumor-associated microbiota and metabolites have emerged as potential modulators of radiotherapy outcomes.MethodsThis study included 22 NPC patients stratified into radiotherapy-responsive (R, n = 12) and radiotherapy-non-responsive (NR, n = 10) groups. Tumor tissue and fecal samples were subjected to 16S rRNA sequencing to profile microbiota composition and targeted metabolomics to quantify short-chain fatty acids (SCFAs). The XGBoost algorithm was applied to identify microbial taxa associated with radiotherapy response, and quantitative PCR (qPCR) was used to validate key findings. Statistical analyses were conducted to assess differences in microbial diversity, relative abundance, and metabolite levels between the groups.ResultsSignificant differences in alpha diversity at the species level were observed between the R and NR groups. Bacteroides acidifaciens was enriched in the NR group, while Propionibacterium acnes and Clostridium magna were more abundant in the R group. Machine learning identified Acidosoma, Propionibacterium acnes, and Clostridium magna as key predictors of radiotherapy response. Metabolomic profiling revealed elevated acetate levels in the NR group, implicating its role in tumor growth and immune evasion. Validation via qPCR confirmed the differential abundance of these microbial taxa in both tumor tissue and fecal samples.DiscussionOur findings highlight the interplay between microbiota and metabolite profiles in influencing radiotherapy outcomes in NPC. These results suggest that targeting the microbiota-metabolite axis may enhance radiotherapy efficacy in NPC.
The impact of a PERMA model-based positive psychology intervention on fear of stroke recurrence: a randomized controlled trial
Authors: Luo Yanfang , Su Zhenzhen , Zhu Lingyun , Huang Yujuan , Liu Zhimin , Dechen Wangmo , Xu Bo , Gao Xinyu , Chen Yuping , Qiu Yuyu , Hao Jianru 
Published: February 2025

IntroductionThis study aimed to examine the effects of a positive psychological intervention, grounded in the PERMA model, on fear levels, psychological capital, overall well-being, and quality of life among stroke patients.MethodsA single-blind, two-arm randomized controlled trial with a repeated measures design was conducted at the Affiliated Hospital of Jiangnan University from January to December 2023. A total of 125 patients experiencing fear of stroke recurrence were randomly assigned to either the intervention group (n = 63), which received a positive psychological intervention based on the PERMA model, or the control group (n = 62), which received standard care. We assessed fear levels, psychological capital, well-being, and quality of life at baseline (T0), on the day of discharge (T1), 2 weeks post-discharge (T2), and 4 weeks post-discharge (T3). The scores of the two groups were compared post-intervention using the Generalized Estimation Equation (GEE) model to analyze the effects of time, group membership, and their interaction.ResultsThe intervention group showed statistically significant improvements compared to the control group, including reduced fear levels (T2: t = −2.094, p = 0.038; T3: t = −2.207, p = 0.029), increased psychological capital (T2: t = 2.053, p = 0.042; T3: t = 2.820, p = 0.006), enhanced well-being (T2: t = 2.037, p = 0.044; T3: t = 2.761, p = 0.007), and better quality of life (T2: t = 2.083, p = 0.039; T3: t = 2.453, p = 0.016) at both T2 and T3. Additionally, significant time-related changes were observed in fear levels, psychological capital, well-being, and quality of life (χ2 = 45.275, p &lt; 0.001; χ2 = 37.848, p &lt; 0.001; χ2 = 48.255, p &lt; 0.001; χ2 = 34.231, p &lt; 0.001, respectively). Notably, the interaction effects were statistically significant for well-being (p &lt; 0.05).DiscussionThe PERMA-based intervention had a short-term positive effect, reducing fear levels while enhancing psychological capital, well-being, and quality of life among stroke patients.Clinical Trial Registrationhttps://www.chictr.org.cn/showproj.html?proj=230313.
Further validation of the Chinese short Warwick Edinburgh mental wellbeing scale in the adult population of Macau: an application of classic test theory and item response theory
Authors: Lam Lawrence T. , Lam Mary K. 
Published: February 2025

BackgroundThis study aims to validate the Chinese version of the Short Warwick-Edinburgh Mental Well-being Scale (SWEMWBS) by employing both Classical Test Theory (CTT) and Item Response Theory (IRT) approaches.MethodsData were gathered through a population-based, cross-sectional health survey using an online self-reported questionnaire. The scale underwent Exploratory Factor Analysis (EFA) and Confirmatory Factor Analysis (CFA). Measurement invariance by gender was assessed using standard procedures. The Grade Response Model (GRM) of the IRT analysis was applied to the data, estimating the discrimination and difficulty parameters at different thresholds. The results were analyzed both graphically and through parameter values.ResultsFactor analyses confirmed that a single-factor model of the scale fit the data well, with an overall Eigenvalue of 4.55, explaining 65.0% of the total variance. Model fit statistics were slightly better for males than for females. Measurement invariance examinations also yielded satisfactory Goodness-of-Fit statistics (CFI = 0.940, TFI = 0.910, RMSEA &lt; 0.001) with minimal changes in item loadings and indicator threshold patterns across groups. The IRT results demonstrated high discrimination parameters, ranging from 2.17 to 3.67, and nearly evenly distributed difficulty parameters, ranging from -2.23 to 1.77. Graphical examinations indicated good performance of the scale across the latent trait continuum.ConclusionsThe results indicated that, as a single-factor scale, the instrument exhibits good quality at both the scale and item levels. It has high discriminative power and an adequate response set for assessing a full range of the latent trait, namely mental well-being.
Predicting satisfaction with vocational status among people with serious mental illness in vocational services: the role of perceived skills and support
Authors: Hoter Ishay Gili , Goldzweig Gil , Hasson-Ohayon Ilanit , Gelkopf Marc , Shadmi Efrat , Roe David 
Published: February 2025

BackgroundThis study aimed to explore the role of perceived skills and support in satisfaction with vocational status to better address the vocational needs and recovery goals of individuals with serious mental illness (SMI). It focuses on three service types: individual placement and support (IPS), sheltered workshops, and vocational support centers.MethodThe study is a cross-sectional analysis of the nationwide Israeli PR-PROM project data. A sample of 2,968 participants diagnosed with SMI and their service providers self-reported their perceived skills, support, and satisfaction with vocational status.ResultsWe found perceived skills and support to be primary predictors of vocational satisfaction across all groups. There were significant differences in satisfaction with vocational status among the three service types: IPS users reported higher satisfaction than sheltered workshop and vocational support center users. The service-provider evaluations reflected similar trends, with higher satisfaction ratings for more integrative services. Additionally, discrepancies were observed between participant and provider satisfaction ratings, varying by vocational service type.DiscussionThe study highlights the importance of perceived skills and support in enhancing satisfaction with vocational status for people with SMI regardless of the vocational service type. Whereas objective factors like employment status and salary contribute to vocational rehabilitation and quality of life, subjective perceptions of skills and support play a significant role in satisfaction with vocational status. The results suggest that vocational rehabilitation services should monitor consumers’ perceptions of skills and support to improve their vocational satisfaction, which can promote personal well-being and social integration.
A global survey about undiagnosed rare diseases: perspectives, challenges, and solutions
Authors: Baldovino Simone , Sciascia Savino , Carta Claudio , Salvatore Marco , Cellai Laura L. , Ferrari Gianluca , Lumaka Aimé , Groft Stephen , Alanay Yasemin , Azam Maleeha , Baynam Gareth , Cederroth Helene , la Paz Eva Maria Cutiongco-de , Dissanayake Vajira Harshadeva Weerabaddana , Giugliani Roberto , Gonzaga-Jauregui Claudia , Hettiarachchi Dineshani , Kvlividze Oleg , Landoure Guida , Makay Prince , Melegh Béla , Ozbek Ugur , Pagava Karaman , Puri Ratna Dua , Romero Vaness I. , Scaria Vinod , Jamuar Saumya S. , Shotelersuk Vorasuk , Roccatello Dario , Gahl William A. , Wiafe Samuel A. , Bodamer Olaf , Posada Manuel , Taruscio Domenica 
Published: February 2025

BackgroundUndiagnosed rare diseases (URDs) are a complex and multifaceted challenge, especially in low-and medium-income countries. They affect individuals with unique clinical features and lack a clear diagnostic label. Although the Undiagnosed Diseases Network International (UDNI) definition of URDs is not universally accepted, it is widely recognized.MethodsWe surveyed UDNI members and participants from other countries to explore the challenges posed by URDs and identify possible solutions. Participation in the survey was completely voluntary.ResultsThe survey revealed a need for more consensus on a universally accepted definition for URDs. Still, the UDNI definition gained widespread recognition and serves as a valuable framework for understanding and addressing the challenges of URDs. In addition to national or international networks, fostering a more substantial engagement and resource-sharing ethos among member countries is critical. Despite advances in genomics and diagnostic tools, the diagnostic journey for people living with URDs (PLURDs) remains arduous and often inconclusive. The availability of specialized centers and the utilization of whole exome sequencing (WES) and whole genome sequencing (WGS) vary across countries, with disparities due to healthcare systems, economic status, and government policies. Advocacy groups play a crucial role in supporting PLURDs.ConclusionA unified commitment to prioritizing URDs on the global health agenda, paired with targeted funding, stipulated national strategies, and aligned international cooperation, is imperative to leveling the playing field for the diagnosis and management of URDs and capitalizing on the potential of Advocacy Groups as allies in this endeavor.
Healthcare workers’ knowledge, attitudes, and practices on catheter-associated UTI prevention: influencing factors in an OB/GYN hospital
Authors: Qu Fei , Pang Yanyu , Wang Mei , Liu Xiaojie , Wang Jing , Li Li 
Published: February 2025

BackgroundCatheter-associated urinary tract infection (CAUTI) is a leading cause of hospital-acquired infections globally, with a high prevalence in China, especially in high-risk settings like intensive care and post-operative environments. These infections are influenced by factors such as patient volume, healthcare worker training, and adherence to infection control protocols. Shanghai, as a major healthcare hub, faces unique challenges in CAUTI prevention. Healthcare workers’ knowledge, attitudes, and practices (KAP) play a crucial role in infection control, yet research on factors affecting KAP in obstetrics and gynecology remains limited. The unique patient population and specialized care protocols in these departments present specific challenges, emphasizing the need for deeper insights to enhance prevention strategies.ObjectiveThe objective was to investigate the KAP scores of healthcare workers in an OB/GYN hospital regarding CAUTI prevention and to identify the factors influencing the scores. The ultimate aim is to provide evidence for improving targeted training programs and infection control measures.Methods and participantsA cross-sectional study was conducted in an OB/GYN hospital in Shanghai, China. A total of 327 healthcare workers from various departments completed a self-administered questionnaire assessing the KAP scores related to CAUTI prevention. The questionnaire also gathered data on demographic and professional characteristics, CAUTI training frequency, leadership emphasis on infection control, and other relevant factors. Data were analyzed using univariate and multivariate analyses to identify factors significantly influencing KAP scores.ResultsDoctors scored higher than nurses in the knowledge dimension (p &lt; 0.001), and increased training frequency was significantly associated with higher knowledge scores (p for trend &lt; 0.001). In terms of attitudes, a longer duration of service, more frequent training, and stronger leadership emphasis were all significantly associated with more positive attitudes toward CAUTI prevention (p for trend &lt; 0.001). Strong leadership emphasis on CAUTI prevention was also linked to improved practices (p for trend &lt; 0.001). The multivariate analysis confirmed that factors such as professional role (doctor vs. nurse), years of service, the role of a clinical instructor, the degree of leadership emphasis, training frequency significantly influenced the scores across various KAP dimensions.ConclusionHealthcare workers’ KAP scores toward CAUTI prevention are influenced by factors such as professional role, experience, clinical instruction, leadership emphasis, and training frequency. Tailored, role-specific training and active leadership involvement are essential to improving infection control and reducing CAUTI incidence.