bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive proteomics of CSF, plasma, and urine identify DDC and other biomarkers of early Parkinson’s disease
Authors: Rutledge Jarod , Lehallier Benoit , Zarifkar Pardis , Losada Patricia Moran , Shahid-Besanti Marian , Western Dan , Gorijala Priyanka , Ryman Sephira , Yutsis Maya , Deutsch Gayle K. , Mormino Elizabeth , Trelle Alexandra , Wagner Anthony D. , Kerchner Geoffrey A. , Tian Lu , Cruchaga Carlos , Henderson Victor W. , Montine Thomas J. , Borghammer Per , Wyss-Coray Tony , Poston Kathleen L. 
Published: March 2024

AbstractParkinson’s disease (PD) starts at the molecular and cellular level long before motor symptoms appear, yet there are no early-stage molecular biomarkers for diagnosis, prognosis prediction, or monitoring therapeutic response. This lack of biomarkers greatly impedes patient care and translational research—l-DOPA remains the standard of care more than 50 years after its introduction. Here, we performed a large-scale, multi-tissue, and multi-platform proteomics study to identify new biomarkers for early diagnosis and disease monitoring in PD. We analyzed 4877 cerebrospinal fluid, blood plasma, and urine samples from participants across seven cohorts using three orthogonal proteomics methods: Olink proximity extension assay, SomaScan aptamer precipitation assay, and liquid chromatography–mass spectrometry proteomics. We discovered that hundreds of proteins were upregulated in the CSF, blood, or urine of PD patients, prodromal PD patients with DAT deficit and REM sleep behavior disorder or anosmia, and non-manifesting genetic carriers of LRRK2 and GBA mutations. We nominate multiple novel hits across our analyses as promising markers of early PD, including DOPA decarboxylase (DDC), also known asl-aromatic acid decarboxylase (AADC), sulfatase-modifying factor 1 (SUMF1), dipeptidyl peptidase 2/7 (DPP7), glutamyl aminopeptidase (ENPEP), WAP four-disulfide core domain 2 (WFDC2), and others. DDC, which catalyzes the final step in dopamine synthesis, particularly stands out as a novel hit with a compelling mechanistic link to PD pathogenesis. DDC is consistently upregulated in the CSF and urine of treatment-naïve PD, prodromal PD, and GBA or LRRK2 carrier participants by all three proteomics methods. We show that CSF DDC levels correlate with clinical symptom severity in treatment-naïve PD patients and can be used to accurately diagnose PD and prodromal PD. This suggests that urine and CSF DDC could be a promising diagnostic and prognostic marker with utility in both clinical care and translational research.
Molecular probes targeting HER2 PET/CT and their application in advanced breast cancer
Authors: Gao Fang , Liu Fengxu , Wang Jun , Bi Junfang , Zhai Luoping , Li Dong 
Published: March 2024

Abstract
Purpose
Human epidermal growth factor receptor 2 (HER2)-positive breast cancer cases are among the most aggressive breast tumor subtypes. Accurately assessing HER2 expression status is vital to determining whether patients will benefit from targeted anti-HER2 treatment. HER2-targeted positron emission tomography (PET/CT) is noninvasive, enabling the real-time evaluation of breast cancer patient HER2 status with accuracy.

Methods
We summarize the research progress of PET/CT targeting HER2 in breast cancer, focusing on PET/CT molecular probes targeting HER2 and their clinical application in the management of advanced breast cancer.

Results
At present, a variety of different HER2 targeted molecular probes for PET/CT imaging have been developed, including nucleolin-labeled antibodies, antibody fragments, nanobodies, and peptides of various affinities, among others. HER2-targeted PET/CT can relatively accurately evaluate HER2 expression status in advanced breast cancer patients. It has good performance in the early detection of small HER2-positive lesions, evaluation of HER2 status in lesions that cannot be readily biopsied, evaluation of the heterogeneity of multiple metastases, identification of lesions with altered HER2 status, and evaluation of the efficacy of anti-HER2 drugs.

Conclusion
HER2-targeted PET/CT offers a promising noninvasive approach for real-time assessment of HER2 status，which can be guide targeted treatment for HER2-positive breast cancer patients. Future prospective clinical studies will be invaluable for fully evaluating the importance of HER2-targeted molecular imaging in the management of breast cancer.
Assessment and application of non-technical skills in robotic-assisted surgery: a systematic review
Authors: Mahendran Vimaladhithan , Turpin Laura , Boal Matthew , Francis Nader K. 
Published: March 2024

Abstract
Background
Undeniably, robotic-assisted surgery (RAS) has become very popular in recent decades, but it has introduced challenges to the workflow of the surgical team. Non-technical skills (NTS) have received less emphasis than technical skills in training and assessment. The systematic review aimed to update the evidence on the role of NTS in robotic surgery, specifically focusing on evaluating assessment tools and their utilisation in training and surgical education in robotic surgery.

Methods
A systematic literature search of PubMed, PsycINFO, MEDLINE, and EMBASE was conducted to identify primary articles on NTS in RAS. Messick’s validity framework and the Modified Medical Education Research Study Quality Instrument were utilised to evaluate the quality of the validity evidence of the abstracted articles.

Results
Seventeen studies were eligible for the final analysis. Communication, environmental factors, anticipation and teamwork were key NTS for RAS. Team-related factors such as ambient noise and chatter, inconveniences due to repeated requests during the procedure and constraints due to poor design of the operating room may harm patient safety during RAS. Three novel rater-based scoring systems and one sensor-based method for assessing NTS in RAS were identified. Anticipation by the team to predict and execute the next move before an explicit verbal command improved the surgeon’s situational awareness.

Conclusion
This systematic review highlighted the paucity of reporting on non-technical skills in robotic surgery with only three bespoke objective assessment tools being identified. Communication, environmental factors, anticipation, and teamwork are the key non-technical skills reported in robotic surgery, and further research is required to investigate their benefits to improve patient safety during robotic surgery.
Flow diversion of ruptured intracranial aneurysms: a single-center study with a standardized antithrombotic treatment protocol
Authors: Rantamo Anni , Gallé Camille , Numminen Jussi , Virta Jyri , Tanskanen Päivi , Lindroos Ann-Christine , Resendiz-Nieves Julio , Lehecka Martin , Niemelä Mika , Haeren Roel , Raj Rahul 
Published: March 2024

Abstract

Background
The use of antithrombotic medication following acute flow diversion for a ruptured intracranial aneurysm (IA) is challenging with no current guidelines. We investigated the incidence of treatment-related complications and patient outcomes after flow diversion for a ruptured IA before and after the implementation of a standardized antithrombotic medication protocol.

Methods
We conducted a single-center retrospective study including consecutive patients treated for acutely ruptured IAs with flow diversion during 2015–2023. We divided the patients into two groups: those treated before the implementation of the protocol (pre-protocol) and those treated after the implementation of the protocol (post-protocol). The primary outcomes were hemorrhagic and ischemic complications. A secondary outcome was clinical outcome using the modified Ranking Scale (mRS).

Results
Totally 39 patients with 40 ruptured IAs were treated with flow diversion (69% pre-protocol, 31% post-protocol). The patient mean age was 55 years, 62% were female, 63% of aneurysms were in the posterior circulation, 92% of aneurysms were non-saccular, and 44% were in poor grade on admission. Treatment differences included the use of glycoprotein IIb/IIIa inhibitors (pre-group 48% vs. post-group 100%), and the use of early dual antiplatelets (pre-group 44% vs. 92% post-group). The incidence of ischemic complications was 37% and 42% and the incidence of hemorrhagic complications was 30% and 33% in the pre- and post-groups, respectively, with no between-group differences. There were three (11%) aneurysm re-ruptures in the pre-group and none in the post-group. There were no differences in mortality or mRS 0–2 between the groups at 6 months.

Conclusion
We found no major differences in the incidence of ischemic or hemorrhagic complications after the implementation of a standardized antithrombotic protocol for acute flow diversion for ruptured IAs. There is an urgent need for more evidence-based guidelines to optimize antithrombotic treatment after flow diversion in the setting of subarachnoid hemorrhage.
Locally resolved stress measurement in the ultra-hard composites polycrystalline diamond and polycrystalline cubic boron nitride
Authors: Breidenstein Bernd , Vogel Nils 
Published: March 2024

AbstractCutting tools made of the ultra-hard composites polycrystalline diamond and polycrystalline boron nitride are being used in more and more sectors of machining. Due to the laborious preparation processes such as grinding, brushing, electrical discharge and laser machining, the subsurface of these tools is strongly stressed mechanically and thermally. This also changes the residual stress state in the highly loaded cutting edge area. The measurement of these residual stresses is not possible by established XRD methods due to the highly curved surface of the cutting edge. The measurement method Raman spectroscopy shows high potential for this application, but conversion factors are necessary for the application. These factors enable the conversion of the stress-induced peak shift in the Raman spectrum into absolute residual stress values. Previous conversion factors are mainly based on hydrostatic load cases, which, however, cannot be transferred to the application on cutting tools. In this work, axial load cases were provided by bending and conversion factors were determined by comparing XRD stress measurements and Raman peak shifts. The conversion factors determined were then plotted against existing results from other studies and the causes for the deviations that occurred were determined. By this, for the first time, a conversion factor for an axial load case for cubic boron nitride could be determined and it could be shown that, as for diamond, it differs significantly from the hydrostatic load case.
The fibro-adipogenic progenitor APOD+DCN+LUM+ cell population in aggressive carcinomas
Authors: Cai Lingyi , Kolonin Mikhail G. , Anastassiou Dimitris 
Published: March 2024

AbstractWe identified a progenitor cell population highly enriched in samples from invasive and chemo-resistant carcinomas, characterized by a well-defined multigene signature including APOD, DCN, and LUM. This cell population has previously been labeled as consisting of inflammatory cancer-associated fibroblasts (iCAFs). The same signature characterizes naturally occurring fibro-adipogenic progenitors (FAPs) as well as stromal cells abundant in normal adipose tissue. Our analysis of human gene expression databases provides evidence that adipose stromal cells (ASCs) are recruited by tumors and undergo differentiation into CAFs during cancer progression to invasive and chemotherapy-resistant stages.
A Novel Communication Time-Delay Cooperative Control Method with Switching Event-Triggered Strategy
Authors: Li Dongni , Cao Liang , Pan Yingnan , Xiao Wenbin , Xue Hong 
Published: March 2024

AbstractA novel communication time-delay classification-based method is designed for nonlinear multiagent systems with the finite-time prescribed performance function. The time-delay phenomenon for communication channels between agents is discussed. Then, an improved time-delay classification method is proposed to broaden the standard of classification mechanism by considering the degree of deviation and relative variation of neighbor agents, rather than classifying the delay time into large time-delay and small time-delay. Based on this, the unified Lyapunov-Krasovskii functional and the finite-time performance function are used to solve the large time-delay phenomenon and ensure that the error is within the preset boundary, respectively. Furthermore, a modified switching event-triggered strategy is put forward to reduce the transmission burden, which considers the impact of tracking error to adjust the threshold condition in real-time. Additionally, all signals of the closed-loop systems are bounded. Eventually, two simulation examples verify the validity of the control strategy.
Modeling the effects of species associations and abiotic parameters on the abundance of mosquito species in a Mediterranean wetland
Authors: Shittu Ridwan Adeyemi , Thomas Stephanie Margarete , Roiz David , Ruiz Santiago , Figuerola Jordi , Beierkuhnlein Carl 
Published: March 2024

AbstractRecent outbreaks of the West Nile virus have been reported in southern Spain, a region with important wetland habitats for migratory birds. Here, we analyzed the role of species association and abiotic parameters on the abundance of seven mosquito species in the Doñana National Park, Spain. We applied the Joint Species Distribution Models (JSDM), using the Hierarchical Modeling of Species Communities approach to simultaneously model the effect of habitat type, normalized difference vegetation index, hydroperiod, distance to rivers, land surface temperature, and the abundance of seven mosquito species. We created three models with varied parameters and evaluated the effects of abiotic parameters and species-to-species associations, which served as proxies for species interactions. Our models estimated the species-specific responses of the mosquito community to abiotic parameters. A positive association was identified within the Culex community with potential biotic interactions. However, Ochlerotatus caspius, Ochlerotatus detritus, and Anopheles atroparvus had no statistically significant association with each other and with the Culex species after accounting for the effect of abiotic parameters. We also found that the potential species associations estimated at particular sites and abiotic parameters influenced the model projection for the average abundance of mosquito species. The JSDM will allow spatial projection of the abundance of each mosquito species, which is an important parameter for epidemiological models. The JSDM inference of species association is important because predation, competition, and facilitation affect the distribution and abundance of different species.
Innovative search and imitation heuristics: an agent-based simulation study
Authors: Guida Vittorio , Mittone Luigi , Morreale Azzurra 
Published: March 2024

AbstractProminent research in strategic imitation, exploration, exploitation, and organizational learning identifies imitation as a less costly alternative to experimentation. Yet, its role in the exploration–exploitation dilemma remains underexplored in the literature. This study employs an agent-based model to examine how two distinct agent types—those who imitate and those who experiment—interact and influence each other. The model incorporates the concept of “satisficing” derived from the behavioral theory of the firm, along with insights from research on imitative heuristics. The findings reveal that overcrowding affects both agent types negatively. Imitators suffer from diminished performance due to intensified competition, which increases as more imitators join the system. Meanwhile, explorers are hindered in their attempts at radical innovation due to the presence of other explorers and clusters of imitators. This paper contributes to the field as the first to model individual agents as ‘satisficers’ within a competitive exploration–exploitation framework. By incorporating imitation, it provides novel insights into the dynamics of organizational learning and strategic decision-making.
Effects of different healing agents on acclimatization success of in vitro rooted Garnem (Prunus dulcis × Prunus persica) rootstock
Authors: Ekinci Heydem , Saskin Necla , Ak Bekir Erol , Dogan Birgul Dikmetas 
Published: March 2024

AbstractContinuing productivity of the acclimatization stage of plantlets means successful micropropagation. Due to the free water and high humidity in the culture container environment, poorly developed cuticle results in rapid water loss and drying of plantlets with watery stems and leaves, incomplete stomata, and large intercellular space. When plantlets are exposed to an environmental condition different from the culture medium, problems, such as rapid water loss and drying, may be encountered, and the survival rate of plantlets in vitro may be reduced. The aim of this study was to reduce the stress factors that occur during the acclimatization phase. For this reason, various healing agents have been used. Some of these compounds are ascorbic acid (AsA), salicylic acid (SA), and sodium nitroprusside (SNP). In the study, the response of AsA (100.0 and 200.0 mg L−1), SA (100.0 and 200.0 mg L−1), and SNP (100.0 and 200.0 µM) applications on growth parameters (survival rate (%), node count (pieces per plant), count of leaves (pieces per plant), shoot length (mm), and shoot diameter (mm)) and physiological variable (Soil Plant Analysis Development (SPAD)) were examined. The AsA100 (100.0 mg L−1 ascorbic acid), AsA200 (200.0 mg L−1 ascorbic acid), and SNP100 (100.0 µM sodium nitroprusside) applications resulted in an improvement in node count, leaf count per plant, shoot length, and shoot diameter parameters. The highest shoot length (60.50 ± 7.81 mm) and node count (16.83 ± 1.15 pieces per plantlet) were achieved with the AsA100 application. The maximum stem diameter (2.31 ± 0.37 mm) was determined with the SNP100 application. There were no statistically significant differences found in the survival rate, leaf count, and SPAD parameter. The current study determined that AsA, SA, and SNP applications were effective in regulating several growth parameters in Garnem plantlets and in reducing acclimation stress thereby facilitating adaptation to external conditions.
Monoclonal Antibodies Targeting CGRP to Treat Vestibular Migraine: A Rapid Systematic Review and Meta-Analysis
Authors: Frosolini Andrea , Lovato Andrea 
Published: March 2024

AbstractVestibular migraine (VM), a subtype of migraine characterized by vestibular symptoms, poses a significant diagnostic and therapeutic challenge. This study aimed to evaluate the effectiveness of monoclonal antibodies targeting Calcitonin Gene Related Peptide (CGRP) in the treatment of VM. Therefore, we conducted a rapid systematic review and meta-analysis following PRISMA and Cochrane guidelines. A search of databases (PubMed, Scopus, Cochrane and Google Scholar) was performed in October 2023. Inclusion criteria required original research articles focusing on patients diagnosed with VM and utilizing CGRP-targeting monoclonal antibodies. We performed qualitative assessments of study design, patient characteristics, and outcomes and, for studies with comparable outcome measures, a meta-analysis was conducted. Our search yielded four relevant studies, including cohort studies and a case report, totaling 99 patients. Proper vestibular instrumental tests were employed in half of the studies. Overall, the included studies reported significant improvements in VM symptoms. Our quantitative analysis, focused on migraine symptoms, demonstrated a substantial reduction in Monthly Days with Migraine at 6 months following treatment. No severe adverse drug reactions were reported. In conclusion, this rapid systematic review and meta-analysis provide preliminary evidence for the efficacy of CGRP-targeting monoclonal antibodies in treating Vestibular Migraine. However, the absence of randomized controlled trials and variations in study designs and diagnostic criteria introduce some limitations. Further research is needed, including controlled trials, to establish a more robust evidence base. Nonetheless, this treatment approach offers hope for the effective management of VM, potentially enhancing the well-being of affected individuals and reducing their associated disability.
Reliability of Monte Carlo simulation approach for estimating uniaxial compressive strength of intact rock
Authors: Aladejare Adeyemi Emman , Idowu Kayode Augustine , Ozoji Toochukwu 
Published: March 2024

AbstractThe strength of rock has significant influence on its performance, and is, therefore, a key input during modelling and analysis of mining and geotechnical engineering structures. The uniaxial compressive strength (UCS), which is a popular parameter to quantifying rock strength can be determined in the laboratory using suggested method by International Society of Rock Mechanics (ISRM). However, the laboratory determination of UCS consumes time, it is costly, and sometimes may not be feasible to perform because of different conditions of rock. Hence, this study attempts to employ Monte Carlo simulation (MCS) approach to estimate UCS, and to overcome various uncertainties associated with UCS estimation. To use MCS approach for UCS estimation, block punch index (BPI), Brazilian tensile strength (BTS), point load index (IS(50)), and P-wave velocity (Vp) were selected as the model inputs. A multiple linear regression (MLR) equation was developed and used to predict UCS by the MCS approach. The methodology was applied to estimate UCS using real BPI, BTS, Is(50), and Vp data as inputs. The proposed approach simulated UCS values that are consistent with UCS values measured in the laboratory. The mean of the UCS values simulated through the MCS approach is 119.10 MPa, while the mean of the UCS values measured in the laboratory is 118.42 MPa. In addition, hypothesis testing revealed that the Brazilian tensile strength (BTS) is the parameter with the most influence on UCS of rock for the site investigated.
A new species of Lasjia (Proteaceae) from Sulawesi: Lasjia griseifolia Utteridge & Brambach
Authors: Utteridge Timothy M. A. , Trethowan Liam A. , Brown Matilda J. M. , Ratcliffe Seth , Plummer Jack , Brambach Fabian , Rustiami Himmah 
Published: March 2024

SummaryLasjia griseifolia Utteridge & Brambach, a member of the Proteaceae, is described and illustrated as a new species from the Indonesian island of Sulawesi. The new species is similar to L. erecta, also from Sulawesi, and morphological differences between the taxa are discussed; a line illustration and detailed notes on the conservation status are provided.