bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rare cause of amenorrhea: Hematocolpos in a post‐cystocele repair patient
Authors: Mohammed Tibyan Noorallah , Elgadi Ammar , Nuri Gamal Mohi Eldeen 
Published: April 2024

Key Clinical MessageSecondary hematocolpos is a rare but serious complication after cystocele repair. This warrants multidisciplinary management (gynecology and urology) to optimize outcomes & minimize iatrogenic risks.AbstractHematocolpos is the term used for the accumulation of menstrual blood in the vagina. It is most commonly seen in adolescent girls but can also occur in elderly women as a result of gynecological conditions. We present the case of a 48‐year‐old female who presented with amenorrhea and abdominal pain following surgical repair of a cystocele. Investigation revealed secondary hematocolpos due to surgical trauma. Surgical drainage and correction of the uterus were performed, resulting in immediate pain relief. This case underscores the importance of considering hematocolpos in patients with post‐surgical amenorrhea, and further research is needed to better understand its causes, risk factors, and optimal management strategies.
Kinetic Trapping of an Out‐of‐Equilibrium Dynamic Library of Imines by Changing Solvent
Authors: Valentini Matteo , Ercolani Gianfranco , Di Stefano Stefano 
Published: April 2024

AbstractA well‐behaved dynamic library composed of two imines and corresponding amines was subjected to the action of an activated carboxylic acid (ACA), whose decarboxylation is known to be base promoted, in different solvents, namely CD2Cl2, CD3CN, and mixtures of them. Two non‐equilibrium systems are consequently obtained: i) a dissipative (CD2Cl2) and ii) an out‐of‐equilibrium (CD3CN) dynamic library whose composition goes back to equilibrium after a given time. In the former case, the library is fully coupled with the decarboxylation of the ACA, while in the latter, an energy ratchet operates. In the mixed solvents, the library exhibits a mediated behavior. Interestingly, in the presence of an excess of added ACA, the different behavior of the imine library in the two solvents is expected to manifest only when the excess acid is consumed.
Ruthenium(II)‐Catalyzed Oxidative Annulation of Imidazo[1,5‐a]quinolin‐2‐iums Salts and Internal Alkynes via C−H Bond Activation
Authors: Konwar Monuranjan , Hazarika Nitumoni , Sarmah Bikash Kumar , Das Animesh 
Published: April 2024

AbstractRuthenium(II)‐catalyzed synthesis of π‐conjugated fused imidazo[1,5‐a]quinolin‐2‐ium derivatives have been achieved via C−H activation of quinoline‐functionalized NHC (NHC=N‐heterocyclic carbene) and oxidative coupling with internal alkynes. The reaction occurred with high efficiency, broad substrate scope, tolerates a wide range of functional groups and utilized into a gram‐scale. Synthetic applications of the coupled product have been exemplified in the late‐stage derivatization of various highly functionalized scaffolds. Moreover, most of the annulated products exhibit intense fluorescence and have potential applications in optoelectronic devices. Mechanistic studies have provided insights into the spectroscopic characterization of key five‐membered ruthenacycle intermediate and Ru(0) sandwich species. Based on several control experiments, deuterium‐kinetic isotope effect, and thermodynamic activation parameters the mechanistic finding demonstrated that fused imidazo‐[1,5‐a]quinolin‐2‐ium C(2)−H bond cleavage is the rate‐determining step and ruling out the possibility of reductive elimination for controlling the rate of reaction.
Post‐translational modifications of vertebrate striated muscle myosin heavy chains
Authors: Morales Paula Nieto , Coons Arianna N. , Koopman Amelia J. , Patel Sonu , Chase P. Bryant , Parvatiyar Michelle S. , Pinto Jose R. 
Published: April 2024

AbstractPost‐translational modifications (PTMs) play a crucial role in regulating the function of many sarcomeric proteins, including myosin. Myosins comprise a family of motor proteins that play fundamental roles in cell motility in general and muscle contraction in particular. A myosin molecule consists of two myosin heavy chains (MyHCs) and two pairs of myosin light chains (MLCs); two MLCs are associated with the neck region of each MyHC's N‐terminal head domain, while the two MyHC C‐terminal tails form a coiled‐coil that polymerizes with other MyHCs to form the thick filament backbone. Myosin undergoes extensive PTMs, and dysregulation of these PTMs may lead to abnormal muscle function and contribute to the development of myopathies and cardiovascular disorders. Recent studies have uncovered the significance of PTMs in regulating MyHC function and showed how these PTMs may provide additional modulation of contractile processes. Here, we discuss MyHC PTMs that have been biochemically and/or functionally studied in mammals' and rodents' striated muscle. We have identified hotspots or specific regions in three isoforms of myosin (MYH2, MYH6, and MYH7) where the prevalence of PTMs is more frequent and could potentially play a significant role in fine‐tuning the activity of these proteins.
Structural testing for CUDA programming model
Authors: Luz Helder J. F. , Souza Paulo S. L. , Souza Simone R. S. 
Published: April 2024

SummaryGraphic processors offer an accessible solution for high‐performance computing, addressing challenges across various fields. The Compute Unified Device Architecture (CUDA) programming model has emerged to enhance the performance of general‐purpose applications on graphic processors. However, developing CUDA programs is far from straightforward, and developers' lack of experience in parallel programming has led to numerous issues. This article presents a structural testing model and criteria to improve the quality of CUDA programs. These criteria facilitate the selection of test cases and aid in identifying faults. The ValiCUDA tool was developed to implement and validate this testing model and criteria. This tool instruments and analyzes programs, generating the necessary elements for each testing criterion. It also facilitates program execution and evaluation of criterion coverage. A statistical validation experiment assessed these criteria' effectiveness, cost, and strength metrics. The results demonstrate that the criteria can identify nontrivial faults in CUDA programs and assist testers in their testing endeavors for such applications.
Chiral Heterometallic Cu8Ln4 Complexes with Enantiopure Schiff Base Ligands: Synthesis, Structural, Spectroscopic and Magnetic Studies
Authors: Dermitzaki Despina , Panagiotopoulou Angeliki , Pissas Michael , Psycharis Vassilis , Raptopoulou Catherine P. 
Published: April 2024

AbstractThe enantiomerically pure Schiff base ligands H2L‐S and H2L‐R yield chiral heterometallic dodecanuclear complexes of the form [Cu8Ln4(OH)8(OMe)4(O2CBut)8(L‐S or L‐R)4(H2O)4] where LnIII=Gd (1S), Tb (2S), Dy (3S, 3R), Ho (4S, 4R), Er (5S) or Y (6S, 6R) and H2L=(S or R)‐2‐{[(1‐hydroxypropan‐2‐yl)imino]methyl}‐6‐methoxyphenol. The complexes are isomorphous and crystallize in the non‐centrosymmetric polar space group C2 in enantiomeric conformation. The chirality of the Schiff base ligands originates from the respective S‐ or R‐ enantiomer of 2‐aminopropan‐1‐ol, is imparted to the complexes and to the crystals that belong to non‐centrosymmetric space group. The chirality and enantiomeric conformation of all complexes are retained in dmso solutions as confirmed by Circular Dichroism spectra which consist of mirror images, expected for enantiomeric pairs. All complexes consist of four distorted cubane‐like subunits [Cu2Ln2(μ3‐OH)2(μ3‐OMe)(μ3‐OR)], which share the LnIII ions and result in a cyclic distorted tetragonal arrangement; each edge of the {LnIII4} quadrilateral is occupied by two μ‐OH− ions that further bridge to a CuII ion. Magnetic susceptibility measurements revealed ferromagnetic interactions for 3S with LnIII=Dy and antiferromagnetic interactions for all other complexes. AC susceptibility data of 3S under 1 kOe external dc field indicate slow magnetic relaxation phenomena below 2 K.
The evaluation of secondary seed dormancy potentials of spring Brassica napus L. genotypes and the relationship with seed germination, vigor, and seed quality traits
Authors: Brown Caroline H. , Gulden Robert H. , Shirtliffe Steven J. , Vail Sally L. 
Published: April 2024

AbstractVolunteer canola (Brassica napus L.) is defined as canola germinating and emerging after the intended cropping season, and it continually ranks as a top occurring weed on the Canadian prairies. Seed dormancy is the physiological mechanism enabling the seed to remain viable in the soil seedbank for extended periods of time. Specifically, canola has a high likelihood of being induced into secondary seed dormancy under adverse environmental conditions not suitable for germination. A reduction of secondary seed dormancy in modern canola varieties may result in lower seedbank persistence; however, the effect of reducing dormancy on other seed traits is not known. This study examined a diverse collection of spring B. napus genotypes produced in differing maternal environments for secondary seed dormancy as well as seed germination, vigor, and seed quality traits. Genotype contributed the most to total variability (46%), the interactions between genotype and environment had a moderate contribution (28%), and very little contribution from maternal environment alone (4%) was observed for the secondary seed dormancy trait. The remaining variability (22%) was allocated to the residuals. No association was observed between secondary seed dormancy and germination time or seed vigor traits in genotypes examined. Significant associations with secondary seed dormancy were detected for primary dormancy, total protein, seed fiber components, glucosinolates content, seed geographical origin, and fatty acid composition. According to the results of this study, the reduction of secondary seed dormancy as a breeding objective in canola breeding programs is feasible as the trait is largely genetically controlled. Furthermore, direct selection against secondary seed dormancy is unlikely to have adverse effects on canola seed germination and vigor given no associations were detected in the current study.
CD177 drives the transendothelial migration of Treg cells enriched in human colorectal cancer
Authors: Ke Shouyu , Lei Yi , Guo Yixian , Xie Feng , Yu Yimeng , Geng Haigang , Zhong Yiqing , Xu Danhua , Liu Xu , Yu Fengrong , Xia Xiang , Zhang Zizhen , Zhu Chunchao , Ling Wei , Li Bin , Zhao Wenyi 
Published: April 2024

AbstractObjectivesRegulatory T (Treg) cells regulate immunity in autoimmune diseases and cancers. However, immunotherapies that target tumor‐infiltrating Treg cells often induce unwanted immune responses and tissue inflammation. Our research focussed on exploring the expression pattern of CD177 in tumor‐infiltrating Treg cells with the aim of identifying a potential target that can enhance immunotherapy effectiveness.MethodsSingle‐cell RNA sequencing (scRNA‐seq) data and survival data were obtained from public databases. Twenty‐one colorectal cancer patient samples, including fresh tumor tissues, peritumoral tissues and peripheral blood mononuclear cells (PBMCs), were analysed using flow cytometry. The transendothelial activity of CD177+ Treg cells was substantiated using in vitro experiments.ResultsScRNA‐seq and flow cytometry results indicated that CD177 was exclusively expressed in intratumoral Treg cells. CD177+ Treg cells exhibited greater activation status and expressed elevated Treg cell canonical markers and immune checkpoint molecules than CD177− Treg cells. We further discovered that both intratumoral CD177+ Treg cells and CD177‐overexpressing induced Treg (iTreg) cells had lower levels of PD‐1 than their CD177− counterparts. Moreover, CD177 overexpression significantly enhanced the transendothelial migration of Treg cells in vitro.ConclusionsThese results demonstrated that Treg cells with higher CD177 levels exhibited an enhanced activation status and transendothelial migration capacity. Our findings suggest that CD177 may serve as an immunotherapeutic target and that overexpression of CD177 may improve the efficacy of chimeric antigen receptor T (CAR‐T) cell therapy.
Provenance and the U–Pb age constraints on the tuff beds of the Late Jurassic–Early Cretaceous Bazhenovo Formation, West Siberian Basin
Authors: Bulatov Timur , Yermakov Yakov , Kulikova Anna , Gareev Bulat , Spasennykh Mikhail 
Published: April 2024

AbstractThin tuff beds of the Late Jurassic–Early Cretaceous Bazhenovo Formation are laterally widespread in the central part of the West Siberian Basin (ca 0.5 million km2). However, the source of the tuff beds remains unclear. The stratigraphy, geochemistry and geochronology of the tuff beds were investigated to reveal their magmatic origin and potential source region. Most of the tuff beds are recorded in member 4 and can be correlated through the Bazhenovo sequence. Thin‐section petrography and X‐ray diffraction indicate that the tuffs mostly comprise clay minerals and K‐feldspars. Less common minerals are plagioclase, quartz and pyrite. The geochemical composition of the Bazhenovo tuff beds suggests a parental magma origin of andesite/basalt, which came from volcanic arc‐related settings. Considering the results of geochemical studies along with LA‐ICP‐MS zircon U–Pb dating (141.3 ± 0.3/2.8 Ma), the palaeovolcanoes of the Caucasus region or south‐east Mongolia–North‐East China are one of the potential source regions of the tuffs. The record of these tuffs indicates the intensive volcanic eruption during the Volgian–Ryazanian, accompanied by a very low‐sedimentation rate and preservation in a reducing environment. The tuff beds have broad implications as an isochronous marker horizon and constraints for the numerical age of the Jurassic–Cretaceous boundary in the Boreal Realm.
Pre‐contact and post‐colonial ecological legacies shape Surinamese rainforests
Authors: Witteveen Nina H. , White Cheryl , Sánchez‐Martínez Barbara A. , Philip Annemarie , Boyd Femke , Booij Roemer , Christ Reyan , Singh Santosh , Gosling William D. , Piperno Dolores R. , McMichael Crystal N. H. 
Published: April 2024

AbstractDisturbances in tropical forests can have long‐lasting ecological impacts, but their manifestations (ecological legacies) in modern forests are uncertain. Many Amazonian forests bear the mark of past soil modifications, species enrichments, and fire events, but the trajectories of ecological legacies from the pre‐contact or post‐colonial period remain relatively unexplored. We assessed the fire and vegetation history from 15 soil cores ranging from 0 to 10 km from a post‐colonial Surinamese archaeological site. We show that (1) fires occurred from 96 bc to recent times and induced significant vegetation change, (2) persistent ecological legacies from pre‐contact and post‐colonial fire and deforestation practices were mainly within 1 km of the archaeological site, and (3) palm enrichment of Attalea, Oenocarpus and Astrocaryum occurred within 0, 1, and 8 km of the archaeological site, respectively. Our results challenge the notion of spatially extensive and persistent ecological legacies. Instead, our data indicate that the persistence and extent of ecological legacies are dependent on their timing, frequency, type, and intensity. Examining the mechanisms and manifestations of ecological legacies is crucial in assessing forest resilience and Indigenous and local land rights in the highly threatened Amazonian forests.
Facile Synthesis of Hydroquinone Linked 3,3’‐Steroid Dimers via a Modified Mitsunobu Protocol
Authors: Klochowicz Patryk , Morzycki Jacek W. , Ramírez‐Lozano Claudia M. , Santillan Rosa , Czajkowska‐Szczykowska Dorota 
Published: April 2024

AbstractA successful approach for the construction of steroid dimers with a hydroquinone linker under PBu3/TMAD/Et3N/toluene conditions is described. This study provided a new one‐step methodology for the design and synthesis of bis‐steroidal 1,4‐phenylene diethers using mild and effective reagents.
COVID‐19 response in a long‐term care facility for people with epilepsy
Authors: Delazer Luisa , Pressler Noah , Balestrini Simona , Xiao Fenglai , Clayton Lisa M. , Anders‐Cannon Jonny , Salvatierra Rebecca , Henry Ian , Sisodiya Sanjay M. , Sander Josemir W. , Koepp Matthias J. 
Published: April 2024

AbstractObjectiveTo assess asymptomatic rates and severity of SARS‐CoV‐2 infection in people with epilepsy and their healthcare workers in a long‐term care facility which had implemented weekly surveillance testing between April 2020 and June 2022.MethodsQuestionnaires focused on objective and subjective COVID‐19 symptoms for people with epilepsy residing in and their healthcare workers at the Chalfont Centre for Epilepsy in June 2022. Demographic information, comorbidities, and seizure frequency were gathered from medical records. We also collected responses on objective and subjective COVID‐19 symptoms from healthcare workers who participated in a prospective study assessing the reaction to COVID‐19 vaccinations (SAFER).ResultsFifty‐five out of 89 (62%) residents tested positive at least once on weekly PCR testing for SARS‐CoV‐2 during the period of interest; 20 of those (37%) were asymptomatic. In comparison, of those 63 healthcare workers who tested positive at least once on weekly testing during the same period, only four (6%) were asymptomatic. Of the 159 healthcare workers who also participated in the SAFER study, 41 tested positive at least once, and seven (17%) were completely asymptomatic during infection with SARS‐CoV‐2.SignificancePeople with epilepsy living in a long‐term care facility were more likely to present with asymptomatic SARS‐CoV‐2 infections than healthcare workers at the same facility. Despite possible bias in the reporting of subjective symptoms due to management‐by‐proxy, there is no evidence that vulnerable people living in an epilepsy long‐term care facility showed reduced resilience towards infections.Plain Language SummaryPeople with epilepsy living in care home facilities had a surprisingly high degree of asymptomatic infections with SARS‐CoV‐2. Very few residents had severe or fatal outcomes. This is in stark contrast to the widely reported bad outcomes for people without epilepsy in other care homes. People with epilepsy reported significantly less symptoms than their healthcare workers. No changes in seizure frequency during or after infection were observed.
Atypical anorexia nervosa: A scoping review to determine priorities in research and clinical practice
Authors: Beard Jessica , Waller Glenn 
Published: April 2024

AbstractBackgroundThere is currently a limited understanding of the identification, nature, and treatment of Atypical Anorexia Nervosa (AAN). Recent systematic reviews have identified only small numbers of candidate papers, and some areas lack any meaningful review so far – particularly treatment outcomes. A key issue is the lack of clarity in the literature regarding the definition of weight loss criteria.ObjectivesThis scoping review aimed to determine the nature and extent of our knowledge of AAN, in order to assist in the development of future systematic reviews and meta‐analyses, as well as indicating what further research is needed.MethodFollowing the identification of 6747 records, 317 records using the term AAN or a defined equivalent were identified from six databases, including the ‘grey’ literature.ResultsOf the 317 studies, 111 provided participant characteristics, and only 10 provided discrete treatment outcomes. Each of these subsets of the data are tabulated and supported with supplementary material, so that future systematic reviewers can access this resource.DiscussionThe pattern and content of the existing studies allows recommendations to be made regarding future reviews, research and clinical practice. There is a particular need for clear weight/weight loss criteria and adequate interventions.
Optimizing fungal treatment of lignocellulosic agro‐industrial by‐products to enhance their nutritional value
Authors: Benaddou Mohamed , Hajjaj Hassan , Allali Aimad , Moubchir Tarik , Nait M’Barek Hasna , Nafidi Hiba‐Allah , Bin Jardan Yousef A. , Dabiellil Fakhreldeen , Bourhia Mohammed , Chakir Mariyem , Diouri Mohammed 
Published: April 2024

AbstractThis study delves into the dynamic interaction between various fungal strains, substrates, and treatment durations to optimize the nutritional value of these by‐products. Six fungi, including Penicillium chrysogenum, Fusarium sp., Fusarium oxysporum, Fusarium solani, Penicillium crustosum, and Cosmospora viridescens, were evaluated across three substrates: wheat straw (WS), cedar sawdust (CW), and olive pomace (OP) over treatment periods of 4, 8, and 12 weeks. The study discerned profound impacts of these fungi across multiple parameters, including cellulose variation (C.var), lignin variation (L.var), and in vitro true digestibility variation (IVTD.var). Our results demonstrated that the various fungi had a significant effect on all parameters (p < .001). Noteworthy, F. oxysporum and F. solani emerged as exemplars, displaying notable lignin degradation, cellulose liberation, and IVTD enhancement. Importantly, P. crustosum demonstrated substantial cellulose degradation, exhibiting optimal efficacy in just 4 weeks for all substrates. Notably, F. sp. excelled, yielding favorable results when treating WS. P. chrysogenum achieved optimal outcomes with 8‐week treatment for WS. Both Fusarium sp. and P. chrysogenum exhibited slight cellulose release, with remarkable reduction of WS lignin compared to other substrates. Especially, WS and OP displayed superior digestibility enhancements relative to CW. It should be noted that the treatment duration further shaped these outcomes, as prolonged treatment (12 weeks) fostered greater benefits in lignin degradation and digestibility, albeit with concomitant cellulose degradation. These findings underscore the intricate balance between fungal strains, substrates, and treatment durations in optimizing the nutritional value of lignocellulosic agro‐industrial by‐products. The outcomes of this study lead to the enhancement in the overall value of by‐products, promoting sustainable livestock feed and advancing agricultural sustainability.