bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of SIRT1 genetic variants in young Mexican individuals: relationships with overweight and obesity
Authors: Salazar-García S. , Ibáñez-Salazar A. , Lares-Villaseñor E. , Gaytan Pacheco Noemi , Uresti-Rivera E. , Portales-Pérez D. P. , De la Cruz-Mosso U. , Vargas-Morales J. M. 
Published: April 2024

The high prevalence of obesity in Mexico starting from the early stages of life is concerning and represents a major public health problem. Genetic association studies have reported that single nucleotide variants (SNVs) in SIRT1, an NAD+-dependent deacetylase that plays an important role in the regulation of metabolic cellular functions, are associated with multiple metabolic disorders and the risk of obesity. In the present study, we analyzed the effect that the SNVs rs1467568 and rs7895833 of the SIRT1 gene may have on cardiometabolic risk factors in a young adult population from Mexico. A cross-sectional study was carried out with young adults between the ages of 18 and 25 who had a body mass index (BMI) greater than 18.5 kg/m2. This study included 1122 young adults who were classified into the normal weight (n = 731), overweight group (n = 277), and obesity group (n = 114) according to BMI of whom 405 and 404 volunteers were genotyped for rs1467568 and rs7895833 respectively using TaqMan probes through allelic discrimination assays. We found that the male sex carrying the G allele of rs7895833 had slightly lower BMI levels (p = 0.009). Furthermore, subjects carrying rs1467568 (G allele) showed a 34% lower probability of presenting with hyperbetalipoproteinemia where female carrying rs1467568 had lower levels of total cholesterol (p = 0.030), triglycerides (p = 0.026) and LDL cholesterol (p = 0.013). In conclusion, these findings suggest that the presence of both SNVs could have a non-risk effect against dyslipidemia in the Mexican population.
Investigation of the safety and protective efficacy of an attenuated and marker M. bovis-BoHV-1 combined vaccine in bovines
Authors: Zhang Sen , Liu Guoxing , Zhang Yisheng , Wang Chen , Xu Xiaowen , Zhao Yuhao , Xiang Zhijie , Wu Wenying , Yang Li , Chen Jianguo , Guo Aizhen , Chen Yingyu 
Published: April 2024

Bovine respiratory disease (BRD) is one of the most common diseases in the cattle industry worldwide; it is caused by multiple bacterial or viral coinfections, of which Mycoplasma bovis (M. bovis) and bovine herpesvirus type 1 (BoHV-1) are the most notable pathogens. Although live vaccines have demonstrated better efficacy against BRD induced by both pathogens, there are no combined live and marker vaccines. Therefore, we developed an attenuated and marker M. bovis-BoHV-1 combined vaccine based on the M. bovis HB150 and BoHV-1 gG-/tk- strain previously constructed in our lab and evaluated in rabbits. This study aimed to further evaluate its safety and protective efficacy in cattle using different antigen ratios. After immunization, all vaccinated cattle had a normal rectal temperature and mental status without respiratory symptoms. CD4+, CD8+, and CD19+ cells significantly increased in immunized cattle and induced higher humoral and cellular immune responses, and the expression of key cytokines such as IL-4, IL-12, TNF-α, and IFN-γ can be promoted after vaccination. The 1.0 × 108 CFU of M. bovis HB150 and 1.0 × 106 TCID50 BoHV-1 gG-/tk- combined strain elicited the most antibodies while significantly increasing IgG and cellular immunity after challenge. In conclusion, the M. bovis HB150 and BoHV-1 gG-/tk- combined strain was clinically safe and protective in calves; the mix of 1.0 × 108 CFU of M. bovis HB150 and 1.0 × 106 TCID50 BoHV-1 gG-/tk- strain was most promising due to its low amount of shedding and highest humoral and cellular immune responses compared with others. This study introduces an M. bovis-BoHV-1 combined vaccine for application in the cattle industry.
Case report: Temozolomide induced hypermutation indicates an unfavorable response to immunotherapy in patient with gliomas
Authors: Liu Jiapeng , Hu Shuli , Jiang Haihui , Cui Yong 
Published: April 2024

BackgroundTemozolomide (TMZ) is a key component in the treatment of gliomas. Hypermutation induced by TMZ can be encountered in routine clinical practice, and its significance is progressively gaining recognition. However, the relationship between TMZ-induced hypermutation and the immunologic response remains controversial.Case presentationWe present the case of a 38-year-old male patient who underwent five surgeries for glioma. Initially diagnosed with IDH-mutant astrocytoma (WHO grade 2) during the first two surgeries, the disease progressed to grade 4 in subsequent interventions. Prior to the fourth surgery, the patient received 3 cycles of standard TMZ chemotherapy and 9 cycles of dose-dense TMZ regimens. Genomic and immunologic analyses of the tumor tissue obtained during the fourth surgery revealed a relatively favorable immune microenvironment, as indicated by an immunophenoscore of 5, suggesting potential benefits from immunotherapy. Consequently, the patient underwent low-dose irradiation combined with immunoadjuvant treatment. After completing 4 cycles of immunotherapy, the tumor significantly shrank, resulting in a partial response. However, after a 6-month duration of response, the patient experienced disease progression. Subsequent analysis of the tumor tissue obtained during the fifth surgery revealed the occurrence of hypermutation, with mutation signature analysis attributing TMZ treatment as the primary cause. Unfortunately, the patient succumbed shortly thereafter, with a survival period of 126 months.ConclusionPatients subjected to a prolonged regimen of TMZ treatment may exhibit heightened vulnerability to hypermutation. This hypermutation induced by TMZ holds the potential to function as an indicator associated with unfavorable response to immunotherapy in gliomas.
Systemic immune-inflammation index and all-cause and cause-specific mortality in sarcopenia: a study from National Health and Nutrition Examination Survey 1999-2018
Authors: Zeng Qing-Yue , Qin Yu , Shi Yi , Mu Xing-Yu , Huang Shi-Jun , Yang Yu-Hao , Liu Si-Min , An Zhen-Mei , Li Shuang-Qing 
Published: April 2024

BackgroundSarcopenia, common in the elderly, often linked to chronic diseases, correlates with inflammation.The association between SII and mortality in sarcopenia patients is underexplored, this study investigates this relationship in a U.S. adult cohort.MethodsWe analyzed 1999–2018 NHANES data, focusing on 2,974 adults with sarcopenia. Mortality outcomes were determined by linking to National Death Index (NDI) records up to December 31, 2019. Using a weighted sampling design, participants were grouped into three groups by the Systemic Immune-Inflammation Index (SII). We used Cox regression models, adjusting for demographic and clinical variables, to explore SII’s association with all-cause and cause-specific mortality in sarcopenia, performing sensitivity analyses for robustness.ResultsOver a median follow-up of 9.2 years, 829 deaths occurred. Kaplan-Meier analysis showed significant survival differences across SII groups. The highest SII group showed higher hazard ratios (HRs) for all-cause and cause-specific mortality in both crude and adjusted models. The highest SII group had a higher HR for all-cause(1.57, 1.25–1.98), cardiovascular(1.61, 1.00–2.58), cancer(2.13, 1.32–3.44), and respiratory disease mortality(3.21, 1.66–6.19) in fully adjusted models. Subgroup analyses revealed SII’s association with all-cause mortality across various demographics, including age, gender, and presence of diabetes or cardiovascular disease. Sensitivity analyses, excluding participants with cardiovascular diseases, those who died within two years of follow-up, or those under 45 years of age, largely reflected these results, with the highest SII group consistently demonstrating higher HRs for all types of mortality in both unadjusted and adjusted models.ConclusionOur study is the first to demonstrate a significant relationship between SII and increased mortality risks in a sarcopenia population.
Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinoma
Authors: Sakata Toshihiro , Yoshio Sachiyo , Yamazoe Taiji , Mori Taizo , Kakazu Eiji , Aoki Yoshihiko , Aoyanagi Nobuyoshi , Okamoto Toru , Ito Takanori , Toyoda Hidenori , Kawaguchi Takumi , Ono Yoshihiro , Takahashi Yu , Taketomi Akinobu , Kanto Tatsuya 
Published: April 2024

IntroductionNatural killer (NK) cells play a pivotal role in immune surveillance in the liver. We aimed to identify potential targets for NK cell-mediated immune intervention by revealing the functional molecules on NK cells in HCC patients.MethodsTo evaluate the impact of aging on NK cell phenotypes, we examined NK cells from healthy volunteers (HVs) of various ages. Because ILT2 expression on CD56dim NK cells increased with increasing age, we enrolled age-matched HCC patients and HVs. We determined the NK cell phenotypes in blood mononuclear cells (PBMCs) and intrahepatic lymphocytes (IHLs) from cancerous and non-cancerous tissues. We evaluated cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) of NK cells in vitro.ResultsILT2-positive CD56dim NK cells in PBMCs were increased in HCC patients compared with HVs. In HCC patients, ILT2-positive CD56dim NK cells were increased in cancerous IHLs compared with non-cancerous IHLs and PBMCs. We examined the impact of macrophage migration inhibitory factor (MIF) on ILT2 expression in co-cultures of HCC cells and NK cells. The enhanced expression of ILT2 on CD56dim NK cells from HCC patients was inhibited by masking antibodies against MIF and CXCR4. ILT2-positive CD56dim NK cells exhibited lower capacities for cytotoxicity and ADCC than ILT2-negative cells, which were partially restored by ILT2 blockade.ConclusionsIn HCC patients, ILT2 is a signature molecule for cancerous CD56dim NK cells with impaired cytolytic capacity. The MIF-CXCR4 interaction is associated with ILT2 induction on CD56dim NK cells and ILT2 serves as a target for functional NK cell restoration.
Indices and methods for evaluating gross ecosystem product in sea areas: a case study in Changdao County, China
Authors: Li Wenwen , He Shuai , Chen Erwen , Xia Tao , Jin Yuemei , Tang Boping , Chen Shang 
Published: April 2024

IntroductionCoastal nations heavily rely on their sea areas to achieve sustainable socioeconomic development. Sea areas offer abundant marine ecosystem products and services that are difficult to substitute with industrial goods. Although considerable research has been performed to evaluate the contribution offrom the marine ecosystems to human well-being, systematic and measurable indices are lacking.MethodsTo address this knowledge gap, ecosystem service theory was used to establish a framework for assessing the gross ecosystem product (GEP) of a sea area, which represents the total monetary value of final ecosystem products directly and indirectly used by people within a specific administrative jurisdiction in one year. To evaluate marine GEP, three primary indices, i.e., material products, regulating products, and cultural products, were employed, and they consist of eight secondary indices. Material products in the sea area include aquatic products and oxygen products; regulating products include carbon sequestration, climate regulation, waste treatment, and coastal protection; and cultural products include leisure and recreation, and seascape-added value. The marine GEP was calculated for the administrative sea areas of Changdao County, which is located in the Yellow Sea-Bohai Sea transitional zone.Results and discussionOur analysis indicated that from 2017 to 2019, the marine GEP in Changdao ranged between 32.59 and 33.35 billion Chinese yuan (CNY). Regulating products contributed two-thirds of this total value. However, over the three-year period, the marine GEP underwent a 5.64% decrease in Changdao. Specifically, the value of material products increased by 38.85% while that of regulating and cultural products decreased by 9.16% and 15.87%, respectively. Notably, the spatial distribution of ecosystem product values in Changdao displayed two prominent trends: (1) higher values along the coastal sea areas of islands and lower values in offshore sea areas; and (2) higher values in coastal areas of southern islands and lower values in coastal areas of northern islands. Marine GEP may serve as a comprehensive indicator for assessing the sustainability of marine ecosystem. Combining the assessment of both marine and terrestrial GEP facilitates an understanding of how marine and terrestrial ecosystems interact in coastal regions. Furthermore, the combined use of marine GEP and GDP helps better evaluate and sort the level of green development of coastal nations and regions.
Design and evaluation of a web-based electronic health record for amblyopia
Authors: Naemi Roya , Akbarian Mina , Ebrahimi Maryam , Shahmoradi Leila , Masoomian Babak , Rezayi Sorayya 
Published: April 2024

IntroductionAmblyopia, or lazy eye, is a type of visual impairment in which the eyesight is not complete, even with the use of glasses. For the treatment of this disease, accurate and continuous examinations are needed. Nowadays, patient-centered care, by relying on web-based electronic records for amblyopia, has the potential to reduce treatment costs, increase the quality of care, and improve the safety and effectiveness of treatment. Therefore, the purpose of this study is to design and evaluate an Electronic Health Record (EHR) for patients with amblyopia.MethodsThe present study is applied developmental research. Using a Morgan table as a sampling tool, a straightforward random sampling technique selected 150 records from 1,500 records that were free of flaws. The design of the electronic version proceeded in a cascading manner so that after the design of each part, it was presented to the amblyopia experts, and if approved, the next part was designed. To design this EHR, the C# programming language and MySQL database were used. A system evaluation was performed by entering and recording patient information. For this purpose, the standard Questionnaire of User Interaction Satisfaction (QUIS), consisting of 18 questions, was used.ResultsAccording to the amblyopia EHR data elements, the data of physician and patient, examinations, website members, and members’ roles were determined. After defining the fields and classes that explain the tables, the EHR was designed. The usability evaluation of the system showed that the mean selection of very good and good options by the users of EHRs was over 90%, indicating the patients’ acceptance of web-based EHRs.ConclusionThe design of an EHR for amblyopia is an effective step toward integrating and improving the information management of these patients. It will also enable the storage and retrieval of patients’ information to reduce and facilitate the control of amblyopia complications.
Association between the relative abundance of phyla actinobacteria, vitamin C consumption, and DNA methylation of genes linked to immune response pathways
Authors: Noronha Natália Yumi , Noma Isabella Harumi Yonehara , Fernandes Ferreira Rafael , Rodrigues Guilherme da Silva , Martins Luzania dos Santos , Watanabe Lígia Moriguchi , Pinhel Marcela Augusta de Souza , Mello Schineider Isabelle , Diani Luísa Maria , Carlos Daniela , Nonino Carla Barbosa 
Published: April 2024

IntroductionThere is an emerging body of evidence that vitamin C consumption can modulate microbiota abundance and can also impact DNA methylation in the host, and this could be a link between diet, microbiota, and immune response. The objective of this study was to evaluate common CpG sites associated with both vitamin C and microbiota phyla abundance.MethodsSix healthy women participated in this cohort study. They were divided into two groups, according to the amount of vitamin C they ingested. Ingestion was evaluated using the 24-h recall method. The Illumina 450 k BeadChip was used to evaluate DNA methylation. Singular value decomposition analyses were used to evaluate the principal components of this dataset. Associations were evaluated using the differentially methylated position function from the Champ package for R Studio.Results and discussionThe group with higher vitamin C (HVC) ingestion also had a higher relative abundance of Actinobacteria. There was a positive correlation between those variables (r = 0.84, p = 0.01). The HVC group also had higher granulocytes, and regarding DNA methylation, there were 207 CpG sites commonly related to vitamin C ingestion and the relative abundance of Actinobacteria. From these sites, there were 13 sites hypomethylated and 103 hypermethylated. The hypomethylated targets involved the respective processes: immune function, glucose homeostasis, and general cellular metabolism. The hypermethylated sites were also enriched in immune function-related processes, and interestingly, more immune responses against pathogens were detected. These findings contribute to understanding the interaction between nutrients, microbiota, DNA methylation, and the immune response.
Impact of pharmaceutical validation on prescribing errors in a neonatal intensive care unit. Randomised and controlled study
Authors: Canales-Siguero M. D. , García-Muñoz C. , Caro-Teller J. M. , Piris-Borregas S. , Martín-Aragón S. , Ferrari-Piquero J. M. , Moral-Pumarega M. T. , Pallás-Alonso C. R. 
Published: April 2024

PurposeTo compare the frequency of electronic prescription errors when the prescription was validated by the clinical pharmacist vs. when it was not.MethodsThis prospective randomised controlled study was conducted in three phases. A randomised phase, in which patients were divided into control and intervention groups, and a pre- and post-intervention phase were consecutively performed to analyse the impact of pharmaceutical validation of prescriptions in a neonatal intensive care unit (NICU). This study was performed at a highly complex NICU at a tertiary hospital. All patients born during the study period who were admitted to the NICU, with a stay lasting ≥24 h, and received active pharmacological treatment were included in the study. Pharmaceutical validation was performed according to the paediatric pharmaceutical care model. A high level of validation was selected for this study. In the intervention group, discrepancies found during the review process were communicated to the medical team responsible for the patients and resolved on the same day.ResultsIn total, 240 patients were included in this study. Sixty-two patients were allocated to the pre-intervention (n = 38) or post-intervention (n = 24) groups, and 178 patients were randomly sorted into two groups, control (n = 82 newborns) and intervention (n = 96 newborns). During the randomisation phase, the number of prescription errors detected was significantly lower in the intervention group than that in the control group (129 vs. 270; p &lt; 0.001). Similarly, prescription errors reaching the patient were significantly reduced from 40% (n = 108) in the control group to 1.6% (n = 2) in the intervention group. In the pre- and post-intervention periods, the prescription lines containing prescription errors decreased from 3.4% to 1.5% (p = 0.005).ConclusionsThis study showed that the pharmaceutical validation process decreased both the number of errors in the electronic prescribing tools and the number of prescription errors reaching the patient.
Internal and external self-affirmation resources: validation and assessment of psychometric properties of the spontaneous self-affirmation measure using structural equation modeling
Authors: Rader Lena , Gauggel Siegfried , Drueke Barbara , Weise Lorenz , Forster Saskia Doreen , Mainz Verena 
Published: April 2024

IntroductionPeople use coping strategies such as self-affirmation to manage threats to their self-esteem. In empirical research, self-affirmation often involves recalling personal values, strengths, or relationships to restore moral integrity. Research shows it improves attitude adjustment, resolves cognitive dissonance, and enhances well-being. Some studies stress the importance of distinguishing between different aspects of self-affirmation, like strengths or social relations. These aspects align with concepts in psychotherapy that differentiate between internal and external resource activation, benefiting health, self-esteem, and resilience. The aim of the current study was twofold: first, to independently test the three-factor structure of the Spontaneous Self-affirmation Measure (SSAM), and second, to integrate self-affirmation strategies into a broader resource activation framework as resilience factors. It also examined associations with self-esteem and effects of age, gender, and education on spontaneous self-affirmation.Methods1,100 participants (72% female, age 18–65) were recruited online. The original three-factor structure of the SSAM (with the factors Strengths, Values and Social relations) was examined using structural equation modeling. Further, a theory driven two-factor structure applying an internal and external resources framework was examined, integrating the factors of the SSAM into the taxonomy of resource activation (Internal resources: Strengths and Values; External resources: Social relations).ResultsThe results of confirmatory factor analyses showed that both the original three-factor structure and the complementary two-factor structure with an Internal resources and External resources factor fit the data appropriately. All three factors of the original factor model showed a high reliability (Strengths: ωt = 0.91, Values: ωt = 0.91, Social relations: ωt = 0.92). We also found measurement invariance across age, gender, and education. Furthermore, group differences regarding gender, education and ethnicity in the utilization of spontaneous self-affirmation strategies were apparent. Finally, it was demonstrated that the Internal resources factor of the complementary two-factor model is significantly more strongly correlated with self-esteem than the External Resources factor [z = 12.80, p &lt; 0.001, 95%CIdiff (0.24, 0.33)].DiscussionThe study confirms the validity of both the three-factor and two-factor structures of the SSAM. Integrating self-affirmation into the resource activation framework may facilitate applying findings from self-affirmation studies to clinical contexts.
Cooperative behavior in adolescents: a contribution of empathy and emotional regulation?
Authors: Martínez-Velázquez Eduardo Salvador , Ponce-Juárez Sandra Pamela , Díaz Furlong Alfonso , Sequeira Henrique 
Published: April 2024

AimThis study aims to identify different levels of empathy and emotional regulation along adolescent years and their relationship with cooperative behavior.MethodsEighty healthy males were divided into four age groups: 20 Early Adolescents, 20 Middle Adolescents, 20 Late Adolescents and 20 Adults. Participants responded to empathic and emotional regulation scales, then were assigned to an unknown partner to perform the prisoner’s dilemma paradigm.ResultsThe statistical analyses allowed to distinguish the groups on the basis of the components making up the two scales: scores on the Perspective Taking component were higher for Adults and Late Adolescents participants than for Middle Adolescents and Early Adolescents groups (p &lt; 0.05); scores on the Personal Distress component were higher for Early Adolescents group than for Late Adolescents and Middle Adolescents groups (p &lt; 0.05); scores on the Difficulties engaging in goal directed behavior component were higher for Middle Adolescents and Early Adolescents groups than for Adults group (p &lt; 0.05). We observed differences between groups (p &lt; 0.001) with higher number of cooperation responses in Adults compared to Middle Adolescents (p &lt; 0.05) and Early Adolescents groups (p &lt; 0.001).DiscussionThese findings suggest that the cooperative behavior changes during the different stages of adolescence seem to be related to the development of empathy and emotional regulation components.