bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prospective study of circulating immune complexes in patients with breast cancer
Authors: Bukh A. , Aguado M. T. , Krarup N. , Poulsen H. S. , Nordentoft A. M. , Moller N. P. H. 
Published: February 2007

AbstractLevels of circulating immune complex (cIC) and complement split product C3d were studied in 86 patients with breast cancer (BC), 22 patients with benign breast disease (BD), and 72 age‐ and sex‐matched blood‐bank donors (NC), using solid‐phase CIq‐protein A RIA, CIq‐anti‐IgG RIA, anti‐C3d anti‐IgG RIA, and polyclonal IgM‐rheumatoid factor ELISA for cIC detection. No significant differences in cIC and C3d levels were found between the groups. The incidence of raised cIC levels varied from 4.9 to 8.2% in the BC group and from 4.5 to 22.7% in the BD group in comparison with 2.9 to 3.0% in the NC group. Using the solid‐phase polyclonal IgM‐rheumatoid factor ELISA we found that the cIC levels of patients with stage‐III cancer were significantly higher than those of patients with stage‐I or stage‐II cancer. However, the other tests showed no relationship to tumor burden. Likewise, an effect of mastectomy on the cIC levels was also only detectable by one of the assays, i.e., the post‐mastectomy levels of cIC as measured by the solid‐phase anti‐C3d anti‐IgG RIA were significantly lower than the pre‐mastectomy levels. Serial analyses of cIC and C3d levels were performed pre‐operatively, one month post‐operatively and every 3 months during the first year after mastectomy in 46 of the patients. During a I‐year observation period, 7 patients developed metastatic disease. The occurrence of metastatic disease was not, however, preceded by characteristic changes in serially determined cIC and C3d levels.
Increased frequency of sister chromatid exchanges in lymphocytes of breast cancer patients
Authors: Adhvaryu Sidharth G. , Rawal Urwashi M. , Patel Jayesh V. , Patel Devendra D. , Balar Damodar B. 
Published: February 2007

AbstractSpontaneous and mitomycin‐C (MMC)‐induced rates of sister chromatid exchanges (SCEs) were studied in peripheral blood lymphocytes (PBLs) of 40 patients with cancer of the breast and 40 healthy female volunteers as controls. Spontaneous SCE per cell value in PBL cultures was 7.72 for the breast cancer patients, which was significantly higher (p < 0.001) than the 6.28 SCEs per cell scored for the controls. Eight of the patients were studied a second time, 3 to 6 months following surgical removal of the tumour. A significant (p < 0.05) reduction in SCE per cell value was observed following mastectomy. MMC‐induced frequencies of SCE remained comparable in patients and controls. Cellular kinetics, calculated as average generation time (AGT) from the frequency of cells in Ml, M2 and M3 cycles, were comparable in patients and controls.
Aminoterminal propeptide of type III procollagen in serum—an indicator of clinical behavior of advanced ovarian carcinoma?
Authors: Risteli Leila , Kauppila Antti , Mäkila Ulla‐Maija , Risteli Juha 
Published: February 2007

AbstractIn ovarian carcinoma, elevated serum concentrations of the aminoterminal propeptide of type‐Ill procollagen are related to the clinical stage of the disease and to the presence of ascites, which contains very high concentrations of the pro‐peptide. In a follow‐up of patients with advanced disease, favorable clinical course was associated with normalizing pro‐pep tide values, whereas in progressing disease the values increased several weeks before clinical progression. In stable disease the concentrations were constantly above the reference range. Laminin and type‐IV‐collagen‐related serum antigens were mostly within the reference range.
Inhibitory effect of prolonged administration of cysteamine on experimental carcinogenesis in rat stomach induced by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine
Authors: Tatsuta Masaharu , Iishi Hiroyasu , Yamamura Hisako , Baba Miyako , Mikuni Tomiko , Taniguchi Haruo 
Published: February 2007

AbstractThe effect of cysteamine (2‐aminoethanethiol hydrochloride) on the incidence and histology of gastric adenocarcinomas induced by N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG) was investigated in inbred Wistar rats. Prolonged administration of 25 or 50 mg per kg body weight of cysteamine after treatment with MNNG for 25 weeks significantly reduced the incidence and number of adenocarcinomas of the glandular stomach. Histological examination showed that the adenocarcinomas that did develop in rats treated with these 2 doses of cysteamine had high mucin‐producing activity. Furthermore, treatment with cysteamine caused significant increases in serum gastrin level and gastric acid secretion, together with significant decreases in the antral mucosal pH and the labelling indices of pyloric and oxyntic gland mucosae and gastric cancer. These findings indicate that cysteamine inhibits the development of gastric adenocarcinomas and that its effect may be related to decreasing proliferation of cells in the gastric mucosae.
Thromboplastin release, but not content, correlates with spontaneous metastasis of cancer cells
Authors: Girimstad Ivar Amund , Prydz Hans 
Published: February 2007

AbstractNo previous studies on the possible contribution of cancer‐cell procoagulants to metastasis have fulfilled all the criteria for attaining biologically relevant and readily interpretable data (Grimstad et al., 1986), viz: (I) Spontaneous metastasis from primary tumors should be assessed in syngeneic animals; (2) cloned cell lines should be used to correlate cell properties, because heterogeneity within the cell lines employed is a source of serious error; (3) enough clones, derived from the same original tumor, should be used to identify only non‐random correlations. Observing these criteria, we examined the procoagulant activities of 19 murine fibrosarcoma cell clones and 4 uncloned cell lines with high to moderate or low potential for lung metastases formation. The procoagulant activity found was exclusively of the thromboplastin (tissue factor, factor III) type. It occurred in all cell homogenates, but the quantities did not correlate with metastatic potential. In contrast, all highly to moderately metastatic cell clones and lines from 2 different fibrosarcomas shed thromboplastin activity into the culture medium, whereas no weakly metastatic cells did. Histological examination further supported these indications that release of thromboplastin from cancer cells can promote metastasis by initiating blood clotting and thereby facilitating arrest of the cancer cells in target organ vessels. Examination of a third fibrosarcoma showed that release of thromboplastin activity is not necessary for metastasis in all tumors.
A new experimental metastasis model in athymic nude mice, the human malignant melanoma lox
Authors: Fodstad ØYstein , Aamdal Steinar , McMenamin Mary , Nesland Jahn M. , Pihl Alexander 
Published: February 2007

AbstractThe human tumor line LOX was established as an s.c. xenograft in nude mice from a lymph‐node metastasis of a patient with malignant melanoma, I.v. injection into adult nude mice of single‐cell suspensions prepared from xenografts resulted in progressively growing lung tumor colonies that killed the animals. No difference in colony formation was seen between cells taken from lung colonies and s.c. xenografts. An in vitro cell line, LOX‐L, was established from lung colonies, and the mono layer cells, detached with EDTA, retained the same ability to form experimental lung metastases. In a total of 14 experiments, 82 of 89 mice receiving I × 106 viable tumor cells died with a mean survival time of 34.1 ± 4.8 days. Long‐term passaging in vivo and in vitro did not result in any alteration of the lung‐colonizing potential of the LOX cells, whereas trypsinization of the cells before i.v. injection reduced lung colony formation. The life span was inversely related to the number of LOX cells injected, permitting estimation of the cell kill caused by chemotherapy. Mice injected i.v. with the LOX cells showed the same relative response to cis‐diamminedichloroplatinum (CDDP) and mitozolomide (MZA) as did animals carrying s.c. xenografts. The LOX cells have shown a remarkable stability and similarity to the cells of the patient's tumor with respect to morphology, karyotype and chemosensitivity. The LOX model may be useful for testing effects of therapy on lung micro‐ and macrometastases, and the activity of antimetastatic agents, as well as for studying mechanisms involved in the metastatic process.
Establishment of a human cell line (mono mac 6) with characteristics of mature monocytes
Authors: Ziegler‐Heitbroc H. W. Löms , Thiel Eckhard , Futterer Agnes , Herzog Volker , Wirtz Antje , Riethmüller Gert 
Published: February 2007

AbstractA monocytic cell line, termed Mono Mac, was established from peripheral blood of a patient with monoblastic leukemia. Two clones, designated Mono Mac I and Mono Mac 6, were isolated and both were assigned to the monocyte lineage on the basis of morphological, cytochemical and immunological criteria. Most importantly, the clones express NaF‐sensitive non‐specific‐esterase, produce reactive oxygen and stain with MAb My4. Mono Mac 6, in addition, constitutively exhibits phagocytosis of antibody‐coated erythrocytes in 80% of the cells and reacts with a panel of MAbs that are specific for mature monocytes, i.e., M42, LeuM3, 63D3, Mo2 and UCHMI. By contrast, the monoblastic cell lines U937 and THP‐I are negative for all these markers. Only expression of My4 could be detected after differentiation induced by interferon‐γ (IFN‐γ). Similar treatment of Mono Mac I, however, resulted in staining with all the monocyte‐specific MAbs mentioned above, while IFN‐γ treatment of Mono Mac 6 enhanced antigen expression. In addition, the cells showed an increased frequency of multinucleated cells with a rise from 4.8% to 21.9%. Mono Mac 6 appears to be the only one of the cell lines studied to constitutively express phenotypic and functional features of mature monocytes.
Cancer of the larynx/hypopharynx, tobacco and alcohol: Iarc international case‐control study in Turin and Varese (Italy), Zaragoza and Navarra (Spain), Geneva (Switzerland) and Calvados (France)
Authors: Tuyns A‐J. , Esteve J. , Raymond L. , Berrino F. , Benhamou E. , Blanchet F. , Boffetta P. , Crosignani P. , Moral A. Del , Lehmann W. , Merletti F. , Pequignot G. , Rreou E. , Sancho‐Garnier H. , Terracini B. , Zubiri A. , Zubiri L. 
Published: February 2007

AbstractA case‐control study on larynx and hypopharynx cancer was carried out in 6 populations including the city of Turin and the province of Varese (Italy), the provinces of Navarra and Zara‐goia (Spain), the canton of Geneva (Switzerland), and the departement of Calvados (France). This report presents an analysis of the risk associated with alcohol and tobacco consumption based on 1,147 male cases and 3,057 male population controls. Special attention was given to the study of the risk at various sites of larynx and hypopharynx. The effect of tobacco is similar for all sites and the risk associated with ever smoking is on the order of 10. The risks from alcohol drinking depend on site. They are similar for epilarynx and hypopharynx (RR = 4.3, for more than 80 g/day) and lower for endolarynx (RR = 2.1, For more than 80 g/day). For all sites the risk decreases after quitting (RR = 0.3 after 10 years); exclusive use of filter cigarettes is protective (RR = 0.5 relative to smokers of plain cigarettes only) as is exclusive use of blond tobacco (RR = 0.5 relative to smokers of black tobacco only). Inhalation increases the risk of endolaryngeal cancer but not that of hypopharynx or epilarynx. The relative risks for joint exposure to alcohol and tobacco are consistent with a multiplicative model.
A case‐control study of diet and colo‐rectal cancer in Northern Italy
Authors: La Vecchia Carlo , Negri Eva , Decarli Adriano , D'Avanzo Barbara , Gallotti Liliana , Gentile Antonella , Fkanceschi Silvia 
Published: February 2007

AbstractThe relation between dietary factors and the risk of colo‐rectal cancer was investigated in a case‐control study conducted in Northern Italy on 339 cases of colon cancer, 236 cases of rectal cancer and 778 controls admitted to hospital for acute, non‐neoplastic or digestive disorders. Consistent positive associations were observed with more frequent consumption of starchy foods (pasta or rice) (relative risk, RR = 3.0 for colon and I.S for rectum (or highest vs. lowest fertile) and beef/veal meats (RR = 2.1 for colon, 2.1 for rectum), whereas reduced relative risks were observed in subjects reporting more frequent green vegetable consumption (RR = 0.5 for highest vs. lowest tertile), a few specific vegetable or fruit items, and coffee (RR = 0.6 for highest vs. lowest tertile). Various fats in seasonings were positively, but inconsistently, related to intestinal cancer risk, whereas no association was evident with measures of whole grain foods or alcohol intake. For both intestinal sites, a 4‐ to 5‐fold difference in risk was evident between the extreme quintiles of a simple score obtained by algebraic sum of the A major groups of foods. These findings could not be explained in terms of confounding by socio‐economic status or other major potential distorting factors, are in agreement with the results from previous studies of colo‐rectal cancer in Southern Europe, and are consistent with various aspects of the descriptive epidemiology of intestinal cancer in Italy.
Human papillomavirus, herpes simplex virus and cervical cancer incidence in greenland and denmark. A population‐based cross‐sectional study
Authors: Kjaer S. K. , de Villiers E.‐M , Haugaard B. J. , Christensen R. B. , Teisen C. , Møller K. A. , Poll P. , Jensen H. , Vestergaard B. F. , Lynge E. , Jensen O. M. 
Published: February 2007

AbstractIn Greenland, the incidence of cervical cancer is 5.7 times higher than in Denmark among women aged 20‐39. From Nuuk (Greenland) and Nykftbing Falster (Denmark) a sample of 800 women aged 20‐39 years was drawn at random. A total of 586 and 661 women were investigated in Greenland and Denmark respectively. All women had a gynecological examination including a PAP‐smear and cervical scrape for HPV‐anafysis (filter in situ hybridization). A blood sample was taken for analysis of HSV type‐specific antibodies (ELISA). The percentage of normal smears was identical in the 2 areas (95%). The total HPV 16/18 infection rate was 13% in Denmark and 8.8% in Greenland, and the age‐adjusted prevalence rate in Greenland was only 67% of that in Denmark (95% Cl: 0.05‐0.89). The proportion of HPV 6711 positivity was the same in Greenland and in Denmark (6.7% vs. 7.5%). A significantly higher proportion of the Greenlandic women had HSV‐2 antibodies (68.2%) in comparison with Danish women (30.9%) (p < 0.01). The prevalence of HSV‐I was also higher in Greenland, especially in women aged 20 to 24. Our finding of a higher HPV infection rate in Denmark than in Greenland, opposed to cervical cancer rates, does not support a role for these viruses as determinants of cervical cancer incidence. In contrast, the rate of HSV‐2 infection co‐varies with the observed incidence of cervical cancer. This is in line with the notion that differences in cervical cancer incidence between Denmark and Greenland are determined by aspects of sexual behavior.
A colposcopical lesion of the uterine cervix frequently associated with papillomavirus type 16 as detected by in situ and southern blot hybridization: A cytohistological correlation study
Authors: Morin Carol , Bouchard Celine , Fortier Michel , Levesque Roger , Meisels Alexandra 
Published: February 2007

AbstractA convoluted aceto‐white lesion was observed by colpos‐copy on the uterine cervix of 19 patients. A search for specific types of papillomavirus was undertaken in these lesions which proved histologically to be a vertical association of CIN in the lower strata of the squamous epithelium and typical condy‐loina in the uppermost layers. Previous cell samples correlated with histology in only (I out of the 19 cases, and were not therefore an accurate predictor of his to logic findings. However 16 out of our (9 cases had sufficient cellular changes to warrant colposcopy and biopsy. Papillomavirus caps id antigen was detected by peroxidase‐antiperoxidase staining technique in 12 out of the 19 cases (63.1%). In situ hybridization using biotinylated HPV‐16 DNA probes was positive in 13 out of 18 cases (72.2%). Southern blot hybridization gave the typical Pstl pattern of HPV‐ONA in 8 out of 9 cases examined. The detection of HPV‐DNA and HPV capsid antigen was particularly high in this “convoluted”cervical lesion, suggesting that the virus remains biologically active. It may therefore be assumed that this lesion represents a particular phase of the disease process in which early gene function and late gene expression of the viral genome are markedly manifested. Furthermore, this lesion may well represent a link between the early manifestation of HPV infection (condyloma) and CIN, which constitute a morphologic continuum initiated by papillomviruses.
Glycoprotein P3.58, associated with tumor progression in malignant melanoma, is a novel leukocyte activation antigen
Authors: Hulzmann Bcrnhard , Lkhmann Jiirgen M. , Ziegler‐Heitbrock H. W. Löms , Funke Ilona , Riethülier Gert , Johnson Judith P. 
Published: February 2007

AbstractThe P3.58 antigen is defined by monoclonal antibodies (MAbs) selected to discriminate between benign and malignant melanocytk cells. Its expression in malignant cells has been shown to correlate with an increased risk of metastasis. A survey of a wide range of tissues revealed that, on normal tissue, expression of P3.58 antigen is restricted to a subset of cells involved in the immune response. The antigen was found not only on certain endothelia, but also on activated macro‐phages in vivo and in vitro and as well as on activated B lymphocytes. A comparison with known B‐lymphocyte and leukocyte activation antigens indicated that P3.5B is a novel leukocyte activation antigen. Biochemical analysis of the P3.58 antigen isolated from cells of different histogenic origin indicated that different molecular forms of the antigen exist, apparently depending on the cell type of origin. P3.58 molecules precipitated from tunicamycin‐treated cells were identical in all cell types, suggesting that the variation observed is due to variable N‐glycosylation.
Lymphoma‐specific T‐ and B‐cell responses suggest the involvement of HTLV‐I in virus‐non‐productive lymphomas of a married couple
Authors: Schneider E. Marion , Saal Johannes G. , Mann Dean L. , Pawelec Graham , Schneider Josef , Schlote Wolfgang , Wernet Peter 
Published: February 2007

AbstractThe occurrence of aberrant cellular progeny is not an infrequent event in vivo. Viral infections (Hanto et al., 1981; Gallo, 1984) and/or failures in genetic programming during cell proliferarion may facilitate the manifestation of malignancy in states of incomplete immune surveillance. This is suggested by a high incidence of lymphoid malignancies in immunode‐pressed individuals, as for example after organ transplantation (Penn, 1981). Efficient cellular defense mechanisms which control malignant growth have been postulated. So far, MHC‐restricted, tumor‐specific cytotoxic T cells have not been identified in vivo. Moreover, effector cells with natural killer activity have not consistently been shown to function as general or specific anti‐tumor agents in vivo. Nevertheless, LAK cells are presently under investigation in adoptive immuno‐therapy for tumors. The concept of LAK‐cell treatment is based on the observation that high concentrations of interleu‐kin 2 (IL‐2) induce activation and proliferation of autologous, tumor‐specific cytotoxic cells (Hersey et al., 1981; Lotze et al., 1981). The most promising results have been obtained in mouse tumor models, whereas treatment of human diseases has rarely been successful (Rosenberg et al., 1986).In the present report, high percentages of CD25‐positive T lymphocytes were detected in PB and LN cell suspensions of a patient with a B‐cell NHL. These T cells were successfully enriched in cultures containing low doses of IL‐2. The CTX mediated by these T‐cell lines and a single T‐cell clone derived from them was specific for autologous lymphoma cells as well as for the lymphoma cells from a different individual. Interestingly, an HTLV‐I‐infected tumor‐cell line was recognized also. The specific cellular as well as the humoral immune response. however, was drastically reduced following chemo‐ and radiotherapy. In contrast, non‐specific CTX mediated by aclivated NK‐like cells persisted.
The effects of human recombinant tumor necrosis factor on glioma‐derived cell lines: Cellular proliferation, cytotoxicity, morphological and radioreceptor studies
Authors: Rutka James T. , Giblin Jane R. , Berens M. E. , Bar‐Shiv Enav , Tokuda Kouichi , McCulloch John R. , Rosenblum Mark L. , Eessalu Thomas E. , Aogarwal Bharat B. , Bodell William J. 
Published: February 2007

AbstractTo determine whether tumor necrosis factor is of potential value for the treatment of human malignant gliomas, we studied the effects of human recombinant tumor necrosis factor (rTNF‐α) on the morphology, incorporation of tritiated thymidine, and proliferation of 5 established cell lines derived from human malignant gliomas and 3 normal human brain cell cultures. A radioreceptor analysis for rTNF‐n was performed on all cell fines and cultures. Two of the 5 human glioma cell lines (SF‐188 and U 343 MG‐A) demonstrated a marked decrease (60% or less of untreated controls) in the uptake of tritiated thymidine when treated with rTNF‐α at a concentration of 40 U/ml; rTNF‐α at 100 U/ml had antiprolif‐erative and cytotoxic effects on both cell lines. The growth and proliferation of cell tines SF‐126 and U 251 MG were not affected by rTNF‐α even at high concentrations (5,000 U/ml). The growth and proliferation of SF‐S39 were affected to an intermediate degree. A colony‐forming efficiency assay corroborated the results of the proliferation studies: SF‐126 was relatively resistant (surviving fraction of 0.9 at 500 U/ml) and SF‐188 was relatively sensitive (surviving fraction of 0.08 at 500 U/ml) to the cytotoxic effects of rTNF‐α. Time‐sequence electron microscopy showed that rTNF‐α at a concentration of 500 U/ml caused ultrastructural changes in SF‐ 188, including increased intracytoplasmic vesiculation, swelling and degeneration of mitochondria, loss of cell:cell junctions! complexes, and fragmentation of the plasma membrane. Studies with 125I‐rTNF‐α showed a variable degree of binding in all cell lines and cultures. SF‐188, a highly sensitive cell line, demonstrated the strongest binding of 125I‐rTNF‐α (3,400 receptors/ cell with high affinity; kd = 0.27 nm), while SF‐126, a highly resistant cell line, had the weakest binding (809 receptors/cell; kd = 0.25 nm). We conclude that there is a spectrum of anti‐proliferative and cytotoxic activity among glioma‐derived tumor cell lines exposed to rTNF‐α. An increased number of rTNF‐α receptors appears to be a necessary but insufficient condition to explain the antiproliferative effects observed in some glioma‐derived cell lines.