bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Roles of Arabidopsis Heterotrimeric G-Protein Subunits in Modulating Cell Division in Roots
Authors: Chen Jin-Gui , Gao Yajun , Jones Alan M. 
Published: May 2006

Abstract
Signaling through heterotrimeric G proteins is conserved in diverse eukaryotes. Compared to vertebrates, the simpler repertoire of G-protein complex and accessory components in Arabidopsis (Arabidopsis thaliana) offers a unique advantage over all other multicellular, genetic-model systems for dissecting the mechanism of G-protein signal transduction. One of several biological processes that the G-protein complex regulates in Arabidopsis is cell division. We determined cell production rate in the primary root and the formation of lateral roots in Arabidopsis to define individually the types of modulatory roles of the respective G-protein α- and β-subunits, as well as the heterotrimer in cell division. The growth rate of the root is in part a consequence of cell cycle maintenance in the root apical meristem (RAM), while lateral root production requires meristem formation by founder pericycle cells. Thus, a comparison of these two parameters in various genetic backgrounds enabled dissection of the role of the G-protein subunits in modulation of cell division, both in maintenance and initiation. Cell production rates were determined for the RAM and lateral root formation in gpa1 (Arabidopsis G-protein α-subunit) and agb1 (Arabidopsis G-protein β-subunit) single and double mutants, and in transgenic lines overexpressing GPA1 or AGB1 in agb1 or gpa1 mutant backgrounds, respectively. We found in the RAM that the heterotrimeric complex acts as an attenuator of cell proliferation, whereas the GTP-bound form of the Gα-subunit's role is a positive modulator. In contrast, for the formation of lateral roots, the Gβγ-dimer acts largely independently of the Gα-subunit to attenuate cell division. These results suggest that Arabidopsis heterotrimeric G-protein subunits have differential and opposing roles in the modulation of cell division in roots.
PHO2, MicroRNA399, and PHR1 Define a Phosphate-Signaling Pathway in Plants
Authors: Bari Rajendra , Datt Pant Bikram , Stitt Mark , Scheible Wolf-Rüdiger 
Published: May 2006

Abstract
Inorganic phosphate (Pi)-signaling pathways in plants are still largely unknown. The Arabidopsis (Arabidopsis thaliana) pho2 mutant overaccumulates Pi in leaves in Pi-replete conditions. Micrografting revealed that a pho2 root genotype is sufficient to yield leaf Pi accumulation. In pho2 mutants, Pi does not repress a set of Pi starvation-induced genes, including AtIPS1, AT4, and Pi transporters Pht1;8 and Pht1;9. Map-based cloning identified PHO2 as At2g33770, an unusual E2 conjugase gene. It was recently shown that Pi deprivation induces mature microRNA (miRNA [miR399]) and that overexpression of miR399 in Pi-replete conditions represses E2 conjugase expression and leads to high leaf Pi concentrations, thus phenocopying pho2. We show here that miR399 primary transcripts are also strongly induced by low Pi and rapidly repressed after addition of Pi. PHO2 transcripts change reciprocally to miR399 transcripts in Pi-deprived plants and in miR399 overexpressers. However, responses after Pi readdition and in β-glucuronidase reporter lines suggest that PHO2 expression is also regulated by Pi in a manner unrelated to miR399-mediated transcript cleavage. Expression of miR399 was strongly reduced in Pi-deprived Arabidopsis phr1 mutants, and a subset of Pi-responsive genes repressed in Pi-deprived phr1 mutants was up-regulated in Pi-replete pho2 mutants. This places miR399 and PHO2 in a branch of the Pi-signaling network downstream of PHR1. Finally, putative PHO2 orthologs containing five miR399-binding sites in their 5′-untranslated regions were identified in other higher plants, and Pi-dependent miR399 expression was demonstrated in rice (Oryza sativa), suggesting a conserved regulatory mechanism.
The Gene Controlling the Quantitative Trait LocusEPITHIOSPECIFIER MODIFIER1Alters Glucosinolate Hydrolysis and Insect Resistance inArabidopsis
Authors: Zhang Zhiyong , Ober James A. , Kliebenstein Daniel J. 
Published: May 2006

AbstractGlucosinolates are sulfur-rich plant secondary metabolites whose breakdown products have a wide range of biological activities in plant–herbivore and plant–pathogen interactions and anticarcinogenic properties. In Arabidopsis thaliana, hydrolysis by the enzyme, myrosinase, produces bioactive nitriles, epithionitriles, or isothiocyanates depending upon the plant's genotype and the glucosinolate's structure. A major determinant of this structural specificity is the epithiospecifier locus (ESP), whose protein causes the formation of epithionitriles and nitriles. A quantitative trait locus (QTL) on chromosome 3 epistatically affects nitrile formation in combination with ESP; this QTL has been termed EPITHIOSPECIFIER MODIFIER1 (ESM1). We identified a myrosinase-associated protein as the ESM1 QTL in Arabidopsis using map-based cloning with recombinant inbred lines, natural variation transcriptomic analysis, and metabolic profiling. In planta and in vitro analyses with natural ESM1 alleles, ESM1 knockouts, and overexpression lines show that ESM1 represses nitrile formation and favors isothiocyanate production. The glucosinolate hydrolysis profile change influenced by ESM1 is associated with the ability to deter herbivory by Trichoplusia ni. This gene could provide unique approaches toward improving human nutrition.
A photodimer of a 4-phenyl-4H-pyran
Authors: Yan Hong , Wang Hui-Qin , Ni Cheng-Liang , Song Xiu-Qing 
Published: May 2006

A new cage photodimer, tetraethyl 2,4,8,10-tetramethyl-6,12-diphenyl-3,9-dioxapentacyclo[6.4.0.02,7.04,11.05,10]dodecane-1,5,7,11-tetracarboxylate, C38H44O10, was prepared through [2+2]-photocycloaddition of diethyl 2,6-dimethyl-4-phenyl-4H-pyran-3,5-dicarboxylate in the solid state. The molecular structure was elucidated by X-ray diffraction analysis, 1H NMR, IR and mass spectroscopy, and elemental analysis. The molecule possesses a crystallographically imposed centre of symmetry. The crystal structure is stabilized by weak C—H...O hydrogen-bond interactions.
A “field change” of inhibited apoptosis occurs in colorectal mucosa adjacent to colorectal adenocarcinoma
Authors: Badvie S , Hanna-Morris A , Andreyev H J N , Cohen P , Saini S , Allen-Mersh T G 
Published: May 2006

Background:Colorectal cancer is associated with a “field change” of increased proliferation throughout the colonic and rectal mucosa. Both proliferation and apoptosis are disrupted during carcinogenesis. Whether altered apoptosis contributes to this field change of microscopic abnormality is, however, unclear. Bcl-xL is an anti-apoptotic protein that inhibits apoptosis by preventing release of cytochromec, a recognised pathway to cell death.Aim:To determine whether Bcl-xL inhibition of apoptosis is increased in colorectal mucosa adjacent to colorectal adenocarcinoma over that in normal non-neoplastic colorectal mucosa.Patients:Patients undergoing surgical resection for neoplastic (adenocarcinoma) or non-neoplastic disease of the colorectum (rectal prolapse, diverticular disease or volvulus).Methods:Formalin-fixed, paraffin-wax-embedded surgical colorectal resection specimens were immunostained for Bcl-xL protein. Labelling indices were determined by counting the proportion of positively stained cells in mucosal crypts.Results:85 patients were studied. Bcl-xL immunostaining was most marked in the upper third of mucosal crypts. It occurred in a minority of samples from non-neoplastic colorectal mucosa, but was seen in most mucosal samples adjacent to colorectal adenocarcinoma. Significant increases (p<0.001) were observed in Bcl-xL labelling indices in the mucosa at 1 cm (n = 46, median labelling index 31.8%, interquartile range 8.3–43.9%) and at 10 cm (n = 52, median labelling index 22.0%, interquartile range 0.0–36.3%) from colorectal carcinoma, compared with normal, non-neoplastic colorectal mucosa (n = 22, median labelling index 0.0%, interquartile range 0.0–0.0%).Conclusions:The findings are consistent with a field change of inhibited apoptosis in mucosa adjacent to colorectal carcinoma.
State Administrative Reorganization
Authors: Mathews John M. 
Published: May 2006

Writing in 1919, Mr. W. F. Willoughby declared that “at the present time few reforms of government in the United States are more urgent than that of the reorganization of the administrative services of our state governments, so as to put them upon the integrated or departmental basis.” This need for state administrative reorganization is now generally recognized, not only by political scientists and students of administration, but also by public officials and practical administrators. This is indicated by the large number of governors who, in public messages, have urged upon their legislatures the adoption of measures of administrative reform; by the investigations and reports of efficiency and economy commissions or similar bodies created in many states; and by the laws actually passed providing for administrative reorganization in Illinois, Idaho, Nebraska, Ohio, Washington, Massachusetts, and other states.A movement of this character naturally follows the line of least resistance, and consequently the changes in administrative organization heretofore made have been through statutory enactment rather than through constitutional revision. Inasmuch as, in most states, a faulty organization of the administration is stereotyped in the constitution, thoroughgoing reorganization by mere statutory enactment is practically impossible. The constitutional difficulties which impede the movement for administrative reorganization should warn us against the insertion in the organic law of detailed administrative provisions.
Reorganization of State Government in Oregon
Authors: Barnett James D. 
Published: May 2006

The establishment of the system of direct government in Oregon—the initiative and referendum, the direct primary, and the recall of officers—has been followed by several important movements for the reorganization of representative government in all three departments.The centralization of the state administration is involved in one of the earliest, as it is in the latest, of these movements. In 1909 and 1911 W. S. U'Ren and others of the People's Power League published a proposal for the “concentration of responsibility and power for the enforcement of the laws as nearly as practicable in one public servant,” as it was expressed, by reducing the number of elective officers to two—the governor and the auditor (besides the three proposed “people's inspectors”), and making most of the other state officers as well as the sheriffs and district attorneys both the appointees and the actual subordinates of the governor. The plan was advocated as the means of securing responsibility for the enforcement of the law, economy in administration, and relief from the burden of the ballot. But the proposal was generally condemned by the press as creating “a monster political machine,” a “monarchical” form of government. In view of this opposition and of the fact that the League was at the same time urging radical changes in the legislative department of the state government, the proposal was not submitted to the people.
The Irish Free State Elections of September, 1927
Authors: Pollock James K. 
Published: May 2006

The voters of the Saorstat Eireann went to the polls on September 15 to elect a new Dail. This was the fourth election under the P.R. system and the second election of the year. Despite the importance of the issues involved and the strenuous efforts of the two larger parties, the total poll was only 35,000 votes greater than in the previous June. But a total poll of 1,180,000 votes is a good record for the Free State, even though on a percentage basis it does not appear large. The present inefficient state of the register makes a percentage comparison somewhat misleading.To the student of political institutions the election was interesting because of its bearing on several important matters: proportional representation, the development of the party system, political education, and parliamentary government. Not the least of these was proportional representation. There has been much discussion of P.R. in the Free State, and the Cumann na nGaedheal, or government party, made a promise in the election campaign to modify or abolish the system if returned to office. It remains to be seen whether this pledge can be carried out. But in the meantime it is interesting to point out how the system appears to have operated in the most recent election.Some of the English newspapers undoubtedly exaggerated the evils of the system, the Sunday Express even saying that “one consequence of the election will be the abandonment of an experiment which distorts rather than expresses the opinion of the voters.”
Administrative Law and the Constitution
Authors: Rosenberry Marvin B. 
Published: May 2006

In the constitution of Massachusetts is found the following: “In the government of this commonwealth the legislative department shall never exercise the executive and judicial powers or either of them; the executive shall never exercise the legislative and judicial powers or either of them; the judicial shall never exercise the legislative and executive powers or either of them; to the end it may be a government of laws and not of men.” This is probably the most explicit statement of the doctrine of separation of powers to be found in the constitution of any of the states of this Union. While the doctrine has been set forth in other constitutions in other language, the constitutions of all the states as construed and interpreted have come to have substantially the same meaning. For more than a century, lawyers, courts, political scientists, publicists, and the people generally regarded the separation of the government into coördinate departments as one of the corner-stones of our liberties.Montesquieu, who had no doubt derived his ideas upon the subject from the writings of Locke and a study of English law, in 1748 published his great work, The Spirit of Laws. In this treatise he gave a new exposition of the doctrine of separation of powers and the reasons for it, in a form which gave it wide currency in the English-speaking world; but this exposition was intended by Montesquieu to be a statement of political theory, and was so accepted by political writers of the time.
Political Developments in Italy
Authors: Spencer Henry R. 
Published: May 2006

“Italian unification” is an old phrase, today acquiring new meaning. Whatever Garibaldi would have thought of D'Annunzian and Fascist squadrism, however Cavour would have viewed the modern cult of Machiavelli, avowed by Mussolini, one may be sure that Mazzini would have been shocked at the chauvinist absolutism now proudly boasted by the Fascist régime: absolutism in the sense of an executive guided by its own intuitions and conscience, free from political criticism or parliamentary control; absolutism also in a sense for which English words are wanting, except as we boldly transliterate, and hope that “intransigence,” “totalitarianism,” will be understood to mean an all-inclusive hundredpercentedness. Italy is called by Mussolini “monolithic,” all of one piece, tolerating anti-Fascist criticism no more than other states tolerate treason; all in and for Fascism, nothing against Fascism. Indeed, Fascism, without ceasing to be a party, has become the state. For parties in Edmund Burke's sense there is supposed to be no more need than for the factions Washington abhorred. Opposition such as is normal in the Cavourian scheme of parliamentary government—responsible critics, loyal and ready to take their turn in governing—is superfluous, a mere obstacle or obstruction to efficiency. None is permitted—in parliament, in press, in platform, or even in theory.What has just been said is presented, not by way of judging, but only of summarizing, the attitude firmly (courageously or arrogantly, according to the observer's prejudices) maintained by the powers that be in Italy today. The recent months have seen several important steps taken in the direction of institutional realization of this view.
The Bicameral System in State Legislation
Authors: Barnett James D. 
Published: May 2006

The submission of proposals for the abolition of the state senate to the people of Oregon at the two preceding general elections is occasion for a summary of considerations in reference to the bicameral system of legislation.The bicameral system has been so long and so widely prevalent that until very recently its “necessity” has been almost universally regarded as “a demonstrated truth.” The British legislature, “the mother of parliaments,” is a development from the assembly of “estates.” Five distinct “estates” were present in the “Model Parliament” of 1295, but through the consolidation of interests, the organization of two legislative chambers, the House of Lords and the House of Commons, was soon evolved. The origin of the bicameral system was thus “not owing to any conviction that two houses would work better than either one or three, but was a matter of sheer accident,” and was not “the invention of any clever constitution-maker.” The bicameral system of legislation, generally based upon English precedent, has usually followed the extension of constitutional government, and at present most national legislatures consist of two chambers.
The Land Department as an Administrative Tribunal
Authors: Pierce Charles R. 
Published: May 2006

If, for the moment, we can conceive of Uncle Sam as being Andrew Carnegie, of Carnegie's millions as unimproved real estate, and of Carnegie's intention to die poor, as Uncle Sam's liberal land policy, we can perhaps best picture to ourselves the public land administration in the United States in a nutshell. The government, like Carnegie, is unloading its vast wealth in a manner calculated to do the most good, and it is guarding itself continuously, although often futilely, from being imposed upon and cheated. The ownership of the public domain by the United States is of the highest possible title. There is no one to dispute the government's absolute ownership of it. There are no taxes to pay. The government is subject to no obligation to dispose of its land. It can keep or dispose of the land as it chooses.In 1789 the United States government started as owner of practically all of the Northwest Territory. Later it acquired, what some geographers call the Southwest Territory, by further cession from the States. By purchase, discovery, annexation and conquest the United States acquired further holdings, so that with the exception of Texas and private holdings the government's fee simple title in the public domain extended from the thirteen colonies to the Gulf of Mexico, and from the Atlantic Ocean on the east coast of Florida to the Pacific and the Arctic Oceans.
Organization and Procedure of the Peace Conference
Authors: Fenwick Charles G. 
Published: May 2006

The difficulty of threading one's way through the intricacies of the organization of the Peace Conference is due chiefly to the fact that the conference is from one point of view a continuation of various interallied conferences and councils created for the prosecution of the war. In some cases the functions of these bodies have apparently been taken over by the conference, and in other cases the preëxisting councils and commissions have continued in operation as interallied agencies distinct from the organization of the conference. A brief review of the most important of these agencies will be of assistance, therefore, in making clear the origin and special functions of the conference.The Supreme War Council was created by an agreement between Great Britain, France and Italy at a meeting held at Rapallo, Italy, in the first week of November, 1917. It was composed of the prime ministers and a member of the governments of each of the great powers fighting on the western front. Its purpose was to watch over the general conduct of the war and prepare recommendations for the decision of the governments. It was to be assisted by a permanent central military committee, consisting of Generals Foch, Wilson and Cadorna, but the decisions of these technical advisers were merely to be the basis of recommendations from the War Council to the several governments, leaving the general staffs and military commands of each power responsible to their individual governments. The United States subsequently adhered to the Rapallo agreement, and participated in the meeting of the council at Versailles on December 1, 1917.
The Pluralistic State
Authors: Ellis Ellen Deborah 
Published: May 2006

The doctrine of the pluralistic state has found its most outspoken advocate in this country in Mr. Harold J. Laski,—an Englishman, recently of Harvard University, and more recently still called back to England to the London School of Economics of the University of London. A number of channels of thought have come together in Mr. Laski's present formulation of the doctrine. Among those in England from whom he received much inspiration and suggestion may be mentioned the late Professor Maitland, and Dr. J. Neville Figgis, as well as Mr. Graham Wallas, and Mr. Ernest Barker.Professor Maitland's work in this field is closely associated with that of Dr. Otto Gierke in Germany, to the third volume of one of whose works, Das Deutsche Genossenschaftsrecht, which Professor Maitland translated, he wrote his famous Introduction in which he stated his own views with regard to the real and truly corporate personality, not only of the state but of other social groupings as well. Another of Gierke's works is Die Genossenschaftstheorie, in which it is attempted to show, to quote Dr. Figgis, “how under the facts of modern life the civilian theory of corporations is breaking down on all hands, and that even in Germany, in spite of the deliberate adoption of the Romanist doctrine, the courts and sometimes even the laws are being driven to treat corporate societies as though they were real and not fictitious persons, and to regard such personality as the natural consequence of permanent association, not a mere mark to be imposed or withheld by the sovereign power.”
The Institute for Government Research
Authors: Willoughby W. F. 
Published: May 2006

The Institute for Government Research is an institution incorporated under the laws of the District of Columbia and wholly supported by voluntary private donations. It received its charter on March 13, 1916, and began active operations on October 1 of the same year. Its purpose as set forth in its charter is:“To conduct scientific investigations into the theory and practice of governmental administration, including inquiries into the form of organization and the manner of operation of federal, state and local governmental bodies and offices in the United States of America; the powers, duties, limitations and qualifications of officers; the methods of administration employed; the character and cost of results obtained and the conditions affecting the efficiency and welfare of governmental officers and employees; to carry on such inquiries, directly or with the coöperation of governments, learned societies, institutions of learning or other agencies and individuals and to make public the results of its investigations; to maintain a library for the use of the society or its members and officers and those affiliated with its work; and to prosecute such other inquiries and perform such other services as may tend to the development and application of the principles of efficiency in governmental administration.”