bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune Responses and Resistance against Vibrio parahaemolyticus Induced by Probiotic Bacterium Arthrobacter XE‐7 in Pacific White Shrimp, Litopenaeus vannamei
Authors: Li Jiqiu , Tan Beiping , Mai Kangsen , Ai Qinhui , Zhang Wenbing , Liufu Zhiguo , Xu Wei 
Published: July 2008

Abstract A 63‐d feeding experiment was conducted to determine the effects of dietary supplementation of probiotic bacterium Arthrobacter XE‐7 on immune responses and resistance against Vibrio parahaemolyticus in the Pacific white shrimp, Litopenaeus vannamei. The probiotic bacteria were administered orally at four different doses of 0, 106, 108, and 1010 colony‐forming unit (CFU)/g feed for shrimp. On Day 50, the shrimp were challenged with Vibrio parahaemolyticus by bath. On Days 7, 21, 49, and 63, six shrimp per tank were sampled to take intestine and hemolymph. With increasing dietary supplementation of probiotic bacteria, shrimp mortality decreased from 63.16% (the control) to 55.9% (106 CFU/g feed), 51.75% (108 CFU/g feed), and 51.78% (1010 CFU/g feed), respectively. Vibrio counts in intestine of shrimp fed probiotic bacterium Arthrobacter XE‐7 was generally lower than that in the control shrimp (P < 0.05). The probiotic bacteria generally increased the immune parameters in shrimp, that is, total hemocyte counts, percentage phagocytosis, respiratory burst activity, and serum phenoloxidase activity. The results showed that probiotic bacterium Arthrobacter XE‐7 can be used as probiotic in shrimp feed.
Associations of oral contraceptive use and dietary restraint with bone speed of sound and bone turnover in university-aged women
Authors: Di Giovanni Gioia , Roy Brian D. , Gammage Kimberley L. , Mack Diane , Klentrou Panagiota 
Published: July 2008

The associations of oral contraceptive use and cognitive dietary restraint with bone speed of sound (SOS) and bone turnover were investigated in 100 Canadian university-aged women (18–25 years old). Dietary restraint was assessed using the Dutch Eating Behavior Questionnaire (DEBQ) and daily calcium intake (Ca++) was assessed by the rapid assessment method. Quantitative ultrasound was used to measure SOS at the tibia and radius. Bone formation was estimated from plasma osteocalcin (OC), 25-OH vitamin D, and serum bone-specific alkaline phosphatase (BAP). Bone resorption was determined from serum cross-linked N-teleopeptide of type I collagen (NTx) and plasma C-terminal telopeptide of type I collagen (CTx). Weekly physical activity energy expenditure (WAeq) was assessed using a standardized questionnaire, and height, body mass, relative body fat (%BF), and chest, waist, and hip circumferences were also measured. Participants were divided into low and moderate to high dietary restrainers (LDRs and MDRs, respectively). These groups were further sub-divided into users and non-users of oral contraceptives. All groups had similar age at menarche, body composition, WAeq, and equally low levels of Ca++and vitamin D. Within the non-users of oral contraceptives, MDR exhibited a lower tibial SOS (p ≤ 0.024) and OC (p ≤ 0.009) than LDR. Moreover, amongst the LDR, the oral contraceptive users had a lower tibial SOS (p ≤ 0.015) and BAP (p ≤ 0.002) than non-users. These results show that bone SOS and bone turnover were influenced by oral contraceptives and cognitive dietary restraint among this population of young women independent of body composition and physical activity.
A 26 year physiological description of a National Hockey League team
Authors: Quinney H. A. , Dewart Randy , Game Alex , Snydmiller Gary , Warburton Darren , Bell Gordon 
Published: July 2008

The primary purpose of this investigation was to examine the physiological profile of a National Hockey League (NHL) team over a period of 26 years. All measurements were made at a similar time of year (pre-season) in 703 male (mean age ± SD = 24 ± 4 y) hockey players. The data were analyzed across years, between positions (defensemen, forwards, and goaltenders), and between what were deemed successful and non-successful years using a combination of points acquired during the season and play-off success. Most anthropometric (height, mass, and BMI) and physiological parameters (absolute and relative VO2 peak, relative peak 5 s power output, abdominal endurance, and combined grip strength) showed a gradual increase over the 26 year period. Defensemen were taller and heavier, had higher absolute VO2 peak, and had greater combined grip strength than forwards and goaltenders. Forwards were younger and had higher values for relative VO2 peak. Goaltenders were shorter, had less body mass, a higher sum of skinfolds, lower VO2 peak, and better flexibility. The overall pre-season fitness profile was not related to team success. In conclusion, this study revealed that the fitness profile for a professional NHL ice-hockey team exhibited increases in player size and anaerobic and aerobic fitness parameters over a 26 year period that differed by position. However, this evolution of physiological profile did not necessarily translate into team success in this particular NHL franchise.
Interactions between hepatic lipase and apolipoprotein E gene polymorphisms affect serum lipid profiles of healthy Canadian adults
Authors: Wood Kevin C.M. , Fullerton Morgan D. , El-Sohemy Ahmed , Bakovic Marica 
Published: July 2008

The purpose of this study was to assess the individual and interactive effects between hepatic lipase (LIPC; C-514T, G-250A) and apolipoprotein E (APOE) (E2, E3, E4) gene polymorphisms on levels of plasma lipoprotein cholesterol and triglyceride among healthy, young, Canadian adults (n = 440). All subjects with at least one APOE2 allele had significantly lower low-density lipoprotein cholesterol, total cholesterol, and total cholesterol – high-density lipoprotein cholesterol ratio when compared with those with the APOE3 or APOE4 allele. There were significant differences in the LIPC allele and genotype frequencies between Caucasian (n = 207) and Asian (n = 211) individuals, but ethnicity did not contribute to the variations in circulating lipids. In addition, the lowest triglyceride levels (0.87 ± 0.27 mmol·mL–1) were found in all APOE2 individuals carrying LIPC-514-CC and LIPC-250-GG genotypes, whereas the highest triglyceride levels (1.29 ± 0.34 –1.32 ± 0.32 mmol·mL–1) were found in APOE2 individuals carrying the opposite genotypes, LIPC-514TT and LIPC-250AA. These observations, distinct from the anti-atherogenic effects of APOE2 through the lowering of low-density lipoprotein cholesterol and LIPC on high-density lipoprotein cholesterol, suggest that there is an interactive effect between APOE and LIPC genotypes on plasma triglyceride levels. These results provide the basis for further studies on establishing which genotype combinations would be the most protective against hypertriglyceridemia.
Whole-body skeletal muscle mass is not related to glucose tolerance or insulin sensitivity in overweight and obese men and women
Authors: Kuk Jennifer L. , Kilpatrick Katherine , Davidson Lance E. , Hudson Robert , Ross Robert 
Published: July 2008

The relationship between skeletal muscle mass, visceral adipose tissue, insulin sensitivity, and glucose tolerance was examined in 214 overweight or obese, but otherwise healthy, men (n = 98) and women (n = 116) who participated in various exercise and (or) weight-loss intervention studies. Subjects had a 75 g oral glucose tolerance test and (or) insulin sensitivity measures by a 3 h hyperinsulinemic–euglycemic clamp technique. Whole-body skeletal muscle mass and visceral adipose tissue were measured using a multi-slice magnetic resonance imaging protocol. Total body skeletal muscle mass was not associated with any measure of glucose metabolism in men or women (p > 0.10). These observations remained independent of age and total adiposity. Conversely, visceral adipose tissue was a significant predictor of various measures of glucose metabolism in both men and women with or without control for age and (or) total body fat (p < 0.05). Although skeletal muscle is a primary site for glucose uptake and deposition, these findings suggest that unlike visceral adipose tissue, whole-body skeletal muscle mass per se is not associated with either glucose tolerance or insulin sensitivity in overweight and obese men and women.
The Global Conveyor Belt from a Southern Ocean Perspective
Authors: Iudicone Daniele , Speich Sabrina , Madec Gurvan , Blanke Bruno 
Published: July 2008

Abstract
Recent studies have proposed the Southern Ocean as the site of large water-mass transformations; other studies propose that this basin is among the main drivers for North Atlantic Deep Water (NADW) circulation. A modeling contribution toward understanding the role of this basin in the global thermohaline circulation can thus be of interest. In particular, key pathways and transformations associated with the thermohaline circulation in the Southern Ocean of an ice–ocean coupled model have been identified here through the extensive use of quantitative Lagrangian diagnostics. The model Southern Ocean is characterized by a shallow overturning circulation transforming 20 Sv (1 Sv ≡ 106 m3 s−1) of thermocline waters into mode waters and a deep overturning related to the formation of Antarctic Bottom Water. Mode and intermediate waters contribute to 80% of the upper branch of the overturning in the Atlantic Ocean north of 30°S. A net upwelling of 11.5 Sv of Circumpolar Deep Waters is simulated in the Southern Ocean. Antarctic Bottom Water upwells into deep layers in the Pacific basin, forming Circumpolar Deep Water and subsurface thermocline water. The Southern Ocean is a powerful consumer of NADW: about 40% of NADW net export was found to upwell in the Southern Ocean, and 40% is transformed into Antarctic Bottom Water. The upwelling occurs south of the Polar Front and mainly in the Indian and Pacific Ocean sectors. The transformation of NADW to lighter water occurs in two steps: vertical mixing at the base of the mixed layer first decreases the salinity of the deep water upwelling south of the Antarctic Circumpolar Current, followed by heat input by air–sea and diffusive fluxes to complete the transformation to mode and intermediate waters.
Seasonal Variations in the Circulation over the Middle Atlantic Bight Continental Shelf
Authors: Lentz Steven J. 
Published: July 2008

Abstract
Fits of an annual harmonic to depth-average along-shelf current time series longer than 200 days from 27 sites over the Middle Atlantic Bight (MAB) continental shelf have amplitudes of a few centimeters per second. These seasonal variations are forced by seasonal variations in the wind stress and the cross-shelf density gradient.
The component of wind stress that drives the along-shelf flow over most of the MAB mid- and outer shelf is oriented northeast–southwest, perpendicular to the major axis of the seasonal variation in the wind stress. Consequently, there is not a significant seasonal variation in the wind-driven along-shelf flow, except over the southern MAB shelf and the inner shelf of New England where the wind stress components forcing the along-shelf flow are north–south and east–west, respectively.
The seasonal variation in the residual along-shelf flow, after removing the wind-driven component, has an amplitude of a few centimeters per second with maximum southwestward flow in spring onshore of the 60-m isobath and autumn offshore of the 60-m isobath. The spring maximum onshore of the 60-m isobath is consistent with the maximum river discharges in spring enhancing cross-shelf salinity gradients. The autumn maximum offshore of the 60-m isobath and a steady phase increase with water depth offshore of Cape Cod are both consistent with the seasonal variation in the cross-shelf temperature gradient associated with the development and destruction of a near-bottom pool of cold water over the mid and outer shelf (“cold pool”) due to seasonal variations in surface heat flux and wind stress.
Loop Current and Deep Eddies
Authors: Oey L-Y. 
Published: July 2008

Abstract
In contrast to the Loop Current and rings, much less is known about deep eddies (deeper than 1000 m) of the Gulf of Mexico. In this paper, results from a high-resolution numerical model of the Gulf are analyzed to explain their origin and how they excite topographic Rossby waves (TRWs) that disperse energy to the northern slopes of the Gulf. It is shown that north of Campeche Bank is a fertile ground for the growth of deep cyclones by baroclinic instability of the Loop Current. The cyclones have horizontal (vertical) scales of about 100 km (1000∼2000 m) and swirl speeds ∼0.3 m s−1. The subsequent development of these cyclones consists of two modes, A and B. Mode-A cyclones evolve into the relatively well-known frontal eddies that propagate around the Loop Current. Mode-A cyclone can amplify off the west Florida slope and cause the Loop Current to develop a “neck” that sometimes leads to shedding of a ring; this process is shown to be the Loop Current’s dominant mode of upper-to-deep variability. Mode-B cyclones are “shed” and propagate west-northwestward at speeds of about 2–6 km day−1, often in concert with an expanding loop or a migrating ring. TRWs are produced through wave–eddy coupling originating primarily from the cyclone birthplace as well as from the mode-B cyclones, and second, but for longer periods of 20∼30 days only, also from the mode-A frontal eddies. The waves are “channeled” onto the northern slope by a deep ridge located over the lower slope. For very short periods (≲10 days), the forcing is a short distance to the south, which suggests that the TRWs are locally forced by features that have intruded upslope and that most likely have accompanied the Loop Current or a ring.
Seasonal Influence of Indonesian Throughflow in the Southwestern Indian Ocean
Authors: Zhou Lei , Murtugudde Raghu , Jochum Markus 
Published: July 2008

Abstract
The influence of the Indonesian Throughflow (ITF) on the dynamics and the thermodynamics in the southwestern Indian Ocean (SWIO) is studied by analyzing a forced ocean model simulation for the Indo-Pacific region. The warm ITF waters reach the subsurface SWIO from August to early December, with a detectable influence on weakening the vertical stratification and reducing the stability of the water column. As a dynamical consequence, baroclinic instabilities and oceanic intraseasonal variabilities (OISVs) are enhanced. The temporal and spatial scales of the OISVs are determined by the ITF-modified stratification. Thermodynamically, the ITF waters influence the subtle balance between the stratification and the mixing in the SWIO. As a result, from October to early December an unusual warm entrainment occurs, and the SSTs warm faster than just net surface heat flux–driven warming. In late December and January, the signature of the ITF is seen as a relatively slower warming of SSTs. A conceptual model for the processes by which the ITF impacts the SWIO is proposed.
Determining the Proportion of Children Too Heavy for Age-Appropriate Car Seats in a Practice-Based Research Network
Authors: Basco William T. , Hletko Paul J. , West Lynn , Darden Paul M. 
Published: July 2008

Introduction more information is needed on how trends in childhood weight might influence legally compliant child safety seat (CSS) use. Objective to determine the proportion of child passengers who cannot comply with one state's CSS law because of weight. Method 6 practices in a practice-based research network provided data on patient demographics, heights, and weights. The authors calculated percentages of children above age-appropriate CSS weight cutoffs (20, 40, and 80 pounds, respectively) for the cohort below 6 years. Results there were 717 participants below 6 years of age. Among all infants (<12 months), 16.4% weighed >20 pounds. Among children 12 months to <6 years old, 20% weighed >40 pounds. One weighed >80 pounds. There were no significant differences among racial groups. There was wide variation among practices, but differences did not achieve statistical significance. Conclusion many children in this diverse cohort were too heavy to be compliant with the state CSS law.
Erythroblastic islands: niches for erythropoiesis
Authors: Chasis Joel Anne , Mohandas Narla 
Published: July 2008

AbstractErythroblastic islands, the specialized niches in which erythroid precursors proliferate, differentiate, and enucleate, were first described 50 years ago by analysis of transmission electron micrographs of bone marrow. These hematopoietic subcompartments are composed of erythroblasts surrounding a central macrophage. A hiatus of several decades followed, during which the importance of erythroblastic islands remained unrecognized as erythroid progenitors were shown to possess an autonomous differentiation program with a capacity to complete terminal differentiation in vitro in the presence of erythropoietin but without macrophages. However, as the extent of proliferation, differentiation, and enucleation efficiency documented in vivo could not be recapitulated in vitro, a resurgence of interest in erythroid niches has emerged. We now have an increased molecular understanding of processes operating within erythroid niches, including cell-cell and cell-extracellular matrix adhesion, positive and negative regulatory feedback, and central macrophage function. These features of erythroblast islands represent important contributors to normal erythroid development, as well as altered erythropoiesis found in such diverse diseases as anemia of inflammation and chronic disease, myelodysplasia, thalassemia, and malarial anemia. Coupling of historical, current, and future insights will be essential to understand the tightly regulated production of red cells both in steady state and stress erythropoiesis.
Genomic characterisation, chromosomal assignment and in vivo localisation of the canine High Mobility Group A1 (HMGA1) gene
Authors: Beuing Claudia , Soller Jan T , Muth Michaela , Wagner Sigfried , Dolf Gaudenz , Schelling Claude , Richter Andreas , Willenbrock Saskia , Reimann-Berg Nicola , Winkler Susanne , Nolte Ingo , Bullerdiek Jorn , Escobar Hugo Murua 
Published: July 2008

Abstract

Background
The high mobility group A1 proteins (HMGA1a/HMGA1b) are highly conserved between mammalian species and widely described as participating in various cellular processes. By inducing DNA conformation changes the HMGA1 proteins indirectly influence the binding of various transcription factors and therefore effect the transcription regulation. In humans chromosomal aberrations affecting the HMGA1 gene locus on HSA 6p21 were described to be the cause for various benign mesenchymal tumours while high titres of HMGA1 proteins were shown to be associated with the neoplastic potential of various types of cancer. Interestingly, the absence of HMGA1 proteins was shown to cause insulin resistance and diabetes in humans and mice.
Due to the various similarities in biology and presentation of human and canine cancers the dog has joined the common rodent animal model for therapeutic and preclinical studies. Accordingly, the canine genome was sequenced completely twice but unfortunately this could not solve the structure of canine HMGA1 gene.

Results
Herein we report the characterisation of the genomic structure of the canine HMGA1 gene consisting of 7 exons and 6 introns spanning in total 9524 bp, the in vivo localisation of the HMGA1 protein to the nucleus, and a chromosomal assignment of the gene by FISH to CFA12q11. Additionally, we evaluated a described canine HMGA1 exon 6 SNP in 55 Dachshunds.

Conclusion
The performed characterisations will make comparative analyses of aberrations affecting the human and canine gene and proteins possible, thereby providing a basis for revealing mechanisms involved in HMGA1 related pathogenesis in both species.
Structural conservation versus functional divergence of maternally expressed microRNAs in the Dlk1/Gtl2 imprinting region
Authors: Kircher Martin , Bock Christoph , Paulsen Martina 
Published: July 2008

Abstract

Background
MicroRNAs play an important functional role in post-transcriptional gene regulation. One of the largest known microRNA clusters is located within the imprinted Dlk1/Gtl2 region on human chromosome 14 and mouse chromosome 12. This cluster contains more than 40 microRNA genes that are expressed only from the maternal chromosome in mouse.

Results
To shed light on the function of these microRNAs and possible crosstalk between microRNA-based gene regulation and genomic imprinting, we performed extensive in silico analyses of the microRNAs in this imprinted region and their predicted target genes.
Bioinformatic analysis reveals that these microRNAs are highly conserved in both human and mouse. Whereas the microRNA precursors at this locus mostly belong to large sequence families, the mature microRNAs sequences are highly divergent.
We developed a target gene prediction approach that combines three widely used prediction methods and achieved a sufficiently high prediction accuracy. Target gene sets predicted for individual microRNAs derived from the imprinted region show little overlap and do not differ significantly in their properties from target genes predicted for a group of randomly selected microRNAs. The target genes are enriched with long and GC-rich 3' UTR sequences and are preferentially annotated to development, regulation processes and cell communication. Furthermore, among all analyzed human and mouse genes, the predicted target genes are characterized by consistently higher expression levels in all tissues considered.

Conclusion
Our results suggest a complex evolutionary history for microRNA genes in this imprinted region, including an amplification of microRNA precursors in a mammalian ancestor, and a rapid subsequent divergence of the mature sequences. This produced a broad spectrum of target genes. Further, our analyses did not uncover a functional relation between imprinted gene regulation of this microRNA-encoding region, expression patterns or functions of predicted target genes. Specifically, our results indicate that these microRNAs do not regulate a particular set of genes. We conclude that these imprinted microRNAs do not regulate a particular set of genes. Rather, they seem to stabilize expression of a variety of genes, thereby being an integral part of the genome-wide microRNA gene regulatory network.