bluuebunny/crossref_metadata_2025_split huggingface dataset:
DOSE-RESPONSE STUDY WITH A NEW LH-RH ANALOGUE, D-Ser (TBU)6 LH-RH 1–9 (EA)10 DURING THE FOLLICULAR PHASE OF THE MENSTRUAL CYCLE
Authors: Friedrich E. , Etzrodt A. , Becker H. , Hanker J. P. , Keller E. , Kleisl P. , Pinto V. , Schindler A. E. , Schneider H. P. G. , Vanderbeke O. , Werder H. , Wyss H. I. 
Published: December 2008

ABSTRACT
D-Ser (TBU)6 LH-RH 1–9 (EA)10 (HOE 766) a highly active LH-RH analogue, was studied with regard to its effects on the release of follicle stimulating hormone (FSH), luteinizing hormone (LH) and oestradiol-17β (Oe2) during the follicular phase of the menstrual cycle. Forty-two regularly menstruating women were allowed to five different treatment groups with different doses (1.25 μg; 2.5 μg; 5.0 μg; 10.0 μg; 20.0 μg) of HOE 766 given as intravenous bolus injections and the plasma concentrations of FSH, LH and Oe2 were measured up to 24 h after injection using specific radioimmunoassays. In the majority of cases, peak values of both FSH and LH occurred 4 h after injection being significantly different from pre-injection levels (P < 0.02 in the 1.25 μg treatment group, P < 0.005 for the other treatment groups). Statistical analysis of maximum values as well as the absolute and relative increase in the different treatment groups revealed a dose-dependent effect of HOE 766.
Maximum values of Oe2 occurred 8 h after injection and were found to be significantly different from pre-injection levels (P < 0.005). However, no dose dependent effect was observed. It was concluded that HOE 766 is a potent and long-acting stimulator of FSH, LH and Oe2 release in women. The effect of HOE 766 is dose dependent for FSH and LH but not for Oe2.
EFFECT OF TSH ON cAMP AND cGMP LEVELS IN THYROID CANCERS, ADENOMAS AND NORMAL HUMAN THYROID TISSUE
Authors: Thomas-Morvan Colette 
Published: December 2008

ABSTRACT
The effect of TSH (50 mU/ml) alone or in combination with theophylline (10 mm) on the cAMP level in human thyroid tissue after a 10 min incubation has been studied in 8 normal tissues, 7 adenomas and 10 thyroid cancers (8 differentiated and 1 anaplastic). Our results have shown the lack of responsiveness to TSH in the 2 anaplastic carcinomas studied, as well as in 4 cases among the 8 differentiated cancers studied. In thyroid adenomas, on the contrary, the cAMP increase in response to TSH was close to that observed in normal tissues. In these adenomas, the mean basal cAMP level (0.43 ± 0.07 pmol/mg tissue) was not statistically different from that in normal tissues (0.36 ± 0.06), while in thyroid cancers the mean basal cAMP level (0.93 ± 0.26) was significantly greater than that in normal tissues (P < 0.05).
The cGMP level was measured in the same conditions in 7 normal thyroid tissues and 8 thyroid cancers (7 differentiated and 1 anaplastic). The mean basal cGMP level in these cancers (9 ± 1 fmol/mg tissue) was not statistically different from that in normal tissues (12 ± 2). In response to TSH, no increase of cGMP level was observed in cancers, or in the normal tissues.
The disorders of hormone synthesis previously seen in human thyroid cancers did not appear related to a decreased cAMP content, but might be due to a lack of responsiveness to TSH in undifferentiated cancers and in some cases of differentiated carcinomas.
COMPARISON OF THE METHODS FOR TISSUE TRIIODOTHYRONINE (T3) EXTRACTION AND SUBSEQUENT RADIOIMMUNOASSAY
Authors: Takaishi Masaru , Miyachi Yoshie , Aoki Mariko , Shishiba Yoshimasa 
Published: December 2008

ABSTRACT
Although there have been various reports on tissue T3 concentration, the examination of the quality of radioimmunoassay has not been available. In the present study, we tried to determine whether the available methods for T3 extraction are adequate for the various methods of T3 radioimmunoassays used. T3 was extracted from liver by ethanol extraction or by acid butanol extraction (Flock's method) and the extract was applied to radioimmunoassay either by Seralute T3 column, ANS-double antibody or the ANS-charcoal method. The values of T3 were compared with those obtained by isotope-equilibration method. The dilution curve of ethanol extract was not parallel with that of the standard in ANS-charcoal or ANS-double antibody technique. When the extract was tested by Seralute method, the dilution curve was parallel to the standard, whereas the T3 value obtained with this method was two-fold higher than that with the isotope equilibration technique. The analysis of the ethanol extract suggested that the lipid extracted by ethanol interfered with the assay. The acid butanol extract when tested either by the ANS-double antibody or Seralute method, showed parallelism to the standard curve and gave T3 values almost identical with those by the isotope-equilibration method. When tested by ANS-charcoal method, the dilution curve of the acid butanol extract was not parallel to the standard. Thus, to obtain reliable results, tissue extraction by Flock's method and subsequent T3 radioimmunoassay by either ANS-double antibody or Seralute T3 method are recommended.
FAMILIAL CUSHING'S SYNDROME
Authors: Arce Bartolomé , Licea Manuel , Hung Santiago , Padrón Rubén 
Published: December 2008

ABSTRACT
Cushing's syndrome has been demonstrated in four of seven siblings with clinical manifestations appearing around puberty in three of the four siblings. The only other associated findings in these cases were short stature and disturbed carbohydrate metabolism. Adenomatous hyperplasia of the adrenal glands was demonstrated in 3 of the patients, and a virilizing adrenal carcinoma in the fourth sibling. The pathogenesis of the adrenocortical disorders in these siblings is discussed.
CONGENITAL ADRENAL HYPERPLASIA: SIMULTANEOUS DETERMINATION OF PLASMA ALDOSTERONE AND 17-HYDROXYPROGESTERONE
Authors: Parth K. , Zimprich H. , Swoboda W. , Brunel Roswitha , Bohrn Edith 
Published: December 2008

ABSTRACT
Plasma levels of aldosterne and 17-hydroxyprogesterone were determined in 5 just diagnosed cases of congenital adrenal hyperplasia due to 21-hydroxylase deficiency without salt-loss before onset of therapy, and in 14 children suffering from the same disorder, under glucocorticoid therapy before and after withdrawal of therapy for one week as well as before and after ACTH-stimulation. Furthermore, urinary pregnanetriol, pregnanetriolone, 17-ketosteroids and 17-ketogenic steroids were determined with and without therapy. Serum concentrations of 17-hydroxyprogesterone in patients before onset of therapy were at least 40 times above the normal range (normal range: 0.39 ± 0.23 ng/ml determined in 19 children of age 1–12 years), aldosterone was also elevated (range 16.5–84.4 ng/100 ml, normal aldosterone being 7.3 ± 4.7 ng/ml). In contrast, well controlled patients (judged on urinary pregnanetriol-excretion) had normal aldosterone (one exception) and 17-hydroxyprogesterone levels below 5 ng/ml. Both steroids were well stimulated by ACTH. In 3 poorly controlled cases elevated pregnanetriol and 17-hydroxyprogesterone values up to 130.9 ng/ml were found. Withdrawal of therapy resulted in pronounced rise of aldosterone and 17-hydroxyprogesterone. Exogenous ACTH had no detectable effect in 7 cases leading to further stimulation in the other children. We found an excellent correlation between plasma 17-hydroxyprogesterone and urinary pregnanetriol (r = 0.84, P < 0.003, n = 14). Fairly good correlation also exists between aldosterone and 17-hydroxyprogesterone values (r = 0.65, P < 0.01, n = 14) in patients before the onset and after withdrawal of therapy.
The data confirm the value of 17-hydroxyprogesterone determinations for diagnosis and management of congenital adrenal hyperplasia on the one hand, and the compensatory elevated aldosterone production in the non-salt-losing form of the disorder on the other.
EFFECT OF AMINOGLUTETHIMIDE PHOSPHATE ON IN VITRO OESTRADIOL STIMULATED PROGESTERONE SYNTHESIS BY RABBIT CORPORA LUTEA
Authors: Fuller Gene B. , Markaverich Barry M. , Hobson William C. 
Published: December 2008

ABSTRACT
Slices of rabbit corpora lutea were incubated for 3 and 8 h with oestradiol-17β in the presence or absence of aminoglutethimide phosphate (AGP)2) in order to identify a possible site(s) of action of oestrogen on progesterone synthesis. Oestradiol was unable to increase progesterone biosynthesis above controls in luteal tissue exposed to AGP during either incubation period. AGP alone significantly reduced progesterone concentrations and synthesis from [14C]acetate at both time periods while oestradiol alone increased progesterone mass at 8 h. Changes in 20α-OH progesterone typically paralleled those of progesterone at 3 h but no effect of the inhibitor was seen at 8 h. Except for an increase in 14C-incorporation into cholesterol esters in the AGP-treated groups at 3 h the concentration or synthesis of sterols from labelled acetate did not respond to treatment.
These results suggested that cholesterol side chain cleavage and cholesterol synthesis are not major in vitro sites of action of oestradiol in the rabbit corpus luteum.
IN VIVO INTERACTION BETWEEN STEROIDAL ALKYLATING AGENTS AND OESTROGEN RECEPTORS IN RABBIT UTERI
Authors: Asai Masafusa , Takeuchi Hiroshi , Okada Hiroji 
Published: December 2008

ABSTRACT
The binding of steroidal alkylating agents to specific tissue component is necessary for the selective distribution of these compounds to their target tissues. The in vivo interaction of Estracyt or NSC-112259 with oestrogen receptors may play an important role in their action mechanism.
Both Estracyt and NSC-112259 which were administrated in vivo, gradually reduced the binding of [3H]oestradiol to cytoplasmic oestrogen receptor in rabbit uteri. From this, it was suggested that a negligible amount of oestradiol was released from these compounds and that the oestradiol moiety was useful as a carrier for the nitrogen mustard moiety. And it appeared that the synthesis of a new receptor protein was inhibited by the nitrogen mustard moiety, thereby causing a decrease in the cytoplasmic oestrogen receptor level.
CORD SERUM PROLACTIN IN RELATION TO THE TIME OF THE DAY, THE SEX OF THE NEONATE AND THE BIRTH WEIGHT
Authors: Badawi M. , Van Exter C. , Delogne-Desnoeck J. , Van Meenen F. , Robyn C. 
Published: December 2008

ABSTRACT
Serum prolactin was measured in 581 blood samples collected immediately after birth from 563 single new-born and from 9 pairs of twins.
Cord serum prolactin is high at birth: the mean level is some 5 times higher than in adults. There is no sex difference in cord serum prolactin concentration in the neonate.
Mean serum prolactin is significantly higher at 1.00 and 2.00 a.m. Thus a circadian periodicity in serum prolactin concentration seems to appear already during foetal life well before the organization of sleep rhythm.
Cord serum prolactin increases with birth weight ranging from 1500 g to 3500 g. Thus, the control of levels of circulating prolactin changes during foetal life resulting in a progressive increase starting from mid-pregnancy.
Mean cord serum prolactin at birth is lower in twins than in single newborns. There is no significant difference in serum prolactin between the first and the second twin.
ABSENCE OF POSITIVE FEEDBACK EFFECT OF OESTROGEN ON LH RELEASE IN PATIENTS WITH TESTICULAR FEMINIZATION SYNDROME
Authors: Aono Toshihiro , Miyake Akira , Kinugasa Takayuki , Kurachi Keiichi , Matsumoto Keishi 
Published: December 2008

ABSTRACT
The response of serum LH to exogenous oestrogen administration was studied in 5 patients with testicular feminization syndrome (TFS).
The serum LH levels were elevated in all the patients, while serum testosterone levels were within the normal male range. Serum FSH levels were elevated in 4 patients and normal in one patient. Intravenous administration of 100 μg of LH-RH provoked a further increase in both LH and FSH. Following intravenous injection of 20 mg of conjugated oestrogen (Premarin®), the LH levels were serially determined until 120 h in TFS patients, 5 normal males, and 10 normal females during the mid-follocular phase (D7-9). Both TFS patients and normal males showed no LH release following oestrogen injection in contrast to normal females who displayed a significant increase in LH with a peak at 48 to 56 h after the injection.
These results seem to suggest that the insensitivity of the hypothalamus to androgen in TFS patients do not affect the sex differentiation of the hypothalamus. The possible role of oestradiol conversion from testosterone in the hypothalamus is discussed.
CHANGES IN SERUM TSH LEVEL AFTER INTRAVENTRICULAR INJECTION OF VARIOUS NEUROMEDIATORS IN RATS
Authors: Holak Henryk , Baldys Agata , Jarzab Barbara , Wystrychowski Antoni , Skrzypek Jan 
Published: December 2008

ABSTRACT
The influence of various neuromediators on pituitary TSH secretion in rats has been investigated. Noradrenaline 50 μg/rat, dopamine 50 μg/rat, serotonine-creatinine-sulphate 100 μg/rat, gamma-aminobutyric acid 100 μg/rat, pilocarpine 1 mg/rat, histamine 100 μ/rat were administered into the lateral ventricle of the brain. All agents were dissolved in Parker's fluid. Two control groups of animals were given Parker's fluid and subjected to surgical manipulations, respectively. Plasma TSH level was estimated after 30 min by means of radioimmunoassay.
The increase in the TSH level was observed after the injection of serotonine and noradrenaline (4.0 and 3.1 ng/ml, respectively) as compared with control group (0.7 ng/ml).
RADIOIMMUNOASSAY OF 3,3′,5′-TRIIODOTHYRONINE (REVERSE T3) ON SMALL REUSABLE SEPHADEX COLUMNS
Authors: Faber Jens , Friis Thorkild , Kirkegaard Carsten , Siersbæk-Nielsen Kaj 
Published: December 2008

ABSTRACT
A simple radioimmunoassay for serum reverse triiodothyronine on small reusable Sephadex G 25 fine columns has been developed. The assay was unaffected by serum volumes of up to 100 μl. The recovery of reverse T3 added to serum was in the mean 98.3 ± sem 3.2 % and the coefficient of variation within and between assay determinations 5.4 % and 7.5 %, respectively. The detection limit was 2.2 pg reverse T3/column. Serum reverse T3 in 58 euthyroid controls was in mean 48 ± sd 9 ng/100 ml, and was positively correlated to age (P < 0.001). No overlap was found between the control group, and the hypo- and hyperthyroid group respectively. Three patients with T3-toxicosis had a normal serum rT3.
IODOAMINO ACID COMPOSITION OF POORLY IODINATED HUMAN THYROGLOBULIN FRACTIONATED BY ISOPYCNIC CENTRIFUGATION
Authors: Vignal A. , Nataf B. M. , Tubiana M. 
Published: December 2008

ABSTRACT
The distribution of iodotyrosines and iodothyronines has been studied in poorly iodinated 19 S thyroglobulins isolated by sucrose gradient centrifugation from five human thyroid tissue. The 19 S thyroglobulins have been fractionated by isopycnic centrifugation in 34.5 % rubidium chloride gradient. The distribution of iodoamino acids depends on the total iodine content of each fraction. The variations in MIT1) and DIT residues with increasing levels of iodination are not identical, the elevation of DIT residues being higher than that of MIT. The number of T4 residues increases quite rapidly after 5 atoms of iodine while T3 increases slightly and slowly. It is shown here that, even after fractionation of poorly iodinated thyroglobulin, the synthesis of thyroid hormones is observed at a level of iodine content as low as 1.4 and 1.8 atoms per molecule of thyroglobulin. This shows that the thyroglobulin fractions obtained by isopycnic centrifugation are still heterogeneous, although less than the initial non-fractionated thyroglobulin. In order to explain our data, it must be admitted that a large proportion of thyroglobulin molecules are not iodinated.
INHIBITION OF ANGIOTENSIN III ACTION BY DES-ASP1-,ILEU8-ANGIOTENSIN II IN MAN
Authors: Kono Tsuyoshi , Ikeda Fumitake , Oseko Fumimaro , Nanno Masataka , Imura Hiroo , Endo Jiro 
Published: December 2008

ABSTRACT
In 5 normal men intravenous infusion of 600 ng/kg/min of des-asp1-,ileu8-angiotensin II (AIIIA) inhibited a rise in blood pressure as well as increase in plasma aldosterone caused by an intravenous infusion of 20 or 100 ng/kg/min of des-asp1-angiotensin II (angiotensin III, AIII). This result and our previous study on simultaneous infusions of 600 ng/kg/min of AIIIA and 20 ng/kg/min of angiotensin II (All) in the same 5 normal men demonstrate that this dose of AIIIA antagonizes AIII and All on the adrenal cortex as well as peripheral arterioles and that AIIIA has the same degree of inhibitory effect on the aldosterone-stimulating action of AIII and on that of All in man.
THE EFFECT OF LOCAL TESTICULAR IRRADIATION ON TESTICULAR HISTOLOGY AND PLASMA HORMONE LEVELS IN THE MALE RAT
Authors: Hopkinson C. R. N. , Dulisch B. , Gauss G. , Hilscher W. , Hirschhäuser C. 
Published: December 2008

ABSTRACT
Adult male rats were subjected to local testicular irradiation, plasma hormone levels and testicular histology being quantified at intervals up to 52 days thereafter.
LH and FSH increased coincidently with spermatid but not with spermatocyte or spermatogonia depletion. Testosterone levels seemed to decrease but this effect was not significant. Oestradiol levels showed no significant changes.
From the correlations between the various parameters it was concluded that the lack of inhibin was the main cause of the increase in both LH and FSH and that spermatids provide the signal for production of this non-steroidal inhibitor. The site of inhibin production was not definitively established but the results would be consistent with production of inhibin by the Sertoli cells in association with spermatids.
KININASE INHIBITION BY GLUCAGON
Authors: Olsen Uffe Bang 
Published: December 2008

ABSTRACT
In vitro glucagon (1–100 μg/ml) has been shown to inhibit the degradation of bradykinin by kininases present in kidney microsomes and erythrocytes. Glucagon was less active on kininases present in plasma and was inactive on purified pancreatic carboxypeptidase B. In conscious dogs the intravenous infusion of glucagon (10 μg/min) increased urine flow (160 %) and urine kinin excretion (130%) and decreased urine kallikrein excretion (14 %). It is suggested that in vivo inhibition of kininases might contribute to the biological effects of glucagon.
EFFECT OF PROSTAGLANDIN SYNTHETASE INHIBITORS ON RENIN AND ALDOSTERONE IN MAN ON A NORMAL OR LOW SODIUM DIET
Authors: Norbiato G. , Bevilacqua M. , Raggi U. , Micossi P. , Moroni C. , Fasoli A. 
Published: December 2008

ABSTRACT
The effect of two inhibitors of prostaglandin synthetase activity (acetylsalicylic acid (ASA) and diclofenac sodium (DCFS)) on plasma renin activity (PRA) and plasma aldosterone (PA) was studied in normal subjects kept on a diet with constant sodium and potassium intake or on a low-salt diet for 7 days.
In 9 subjects, 2 days of treatment with ASA (3 g/day) was followed by a significant decrease of PA in the supine position (after overnight rest); there was no significant decrease of PRA. However, both PA and PRA with the subjects in the upright position were significantly reduced after ASA.
In 9 subjects treated with DCFS (200 mg for 2 days), both PA and PRA with the subjects in the supine and upright positions decreased significantly. Similar results were obtained from 4 subjects on a low-sodium diet (15–30 mEq./day) treated with DCFS (150 mg/day for 3 days).
In contrast, no significant changes in PA or PRA with the subjects in the supine or upright position were observed in 4 subjects on a very low sodium diet (< 15 mEq./day) treated with DCFS (150 mg/day for 3 days). Covariance analysis (with PRA as independent variable and PA as dependent variable) of the data obtained in the upright position before and after treatment with ASA or DCFS showed that the decrease in PA remained significant after adjustment for the PRA effect.
These results suggest a direct effect of prostaglandins on PA. The data obtained from subjects on a low sodium diet indicate that sodium deprivation may counteract the effects of DCFS on PRA and PA.
THE SEQUENCE OF HORMONAL CHANGES DURING PROSTAGLANDIN INDUCED LUTEOLYSIS OF THE SUPERLUTEINIZED RAT OVARY
Authors: Torjesen P. A. , Dahlin R. , Haug E. , Aakvaag A. 
Published: December 2008

ABSTRACT
Immature female rats were pre-treated with pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) to achieve superluteinization. Eight days after the HCG administration luteolysis was induced by sc injection of 5 μg of the prostaglandin F2α (PGF2α) analogue cloprostenol (Estrumate®). The serum levels of progesterone, 20α-dihydroprogesterone (20α-DHP), prolactin (PRL) and luteinizing hormone (LH) as well as the number of ovarian LH binding sites were measured during the first 23 h after cloprostenol injection.
The serum levels of progesterone decreased from 500 to 200 ng/ml within 25 min after cloprostenol administration. A further decrease to 20 ng/ml occurred during the next 4 h, and serum progesterone remained low for the rest of the period. An increase in serum prolactin (PRL) to values between 28 and 44 ng/ml was observed after 3 h and the values remained elevated for the next 7 h. Although the serum levels of progesterone declined immediately, the serum 20α-dihydroprogesterone (20α-DHP) levels remained at 60 to 140 ng/ml for the first 5 h and then gradually increased to values corresponding to the initial progesterone levels 14 to 23 h after treatment. The number of ovarian LH binding sites was between 1.2 and 1.4 × 10−12 mol/mg protein during the first 9 h after prostaglandin (PG) injection, and then decrreased to 0.8 and 0.5 × 10−12 mol/mg protein at 14 and 23 h, respectively. The serum LH levels remained below the limit of detection for the assay (10 ng/ml) throughout the observation period. PGF2α injection induced the same basic changes in the serum levels of progesterone and 20α-DHP as cloprostenol treatment.
Thus, the first effect of PG treatment measured was an immediate decline in the serum levels of progesterone, and this decline probably initiated the subsequent increase in pituitay PRL and ovarian 20α-DHP secretion. Therefore, the decrease in the number of ovarian LH binding sites appeared to be a consequence rather than a mediator of luteolytic effects of the prostaglandins.
SERUM LEVELS OF TESTICULAR AND ADRENAL STEROIDS AFTER DEXAMETHASONE AND HCG-ADMINISTRATION IN THE LABORATORY-MAINTAINED RHESUS MONKEY
Authors: Wickings E. J. , Nieschlag E. 
Published: December 2008

ABSTRACT
In order to evaluate the endocrine testicular and adrenal function of rhesus monkeys in vivo, and to compare this with the human situation, the effect of dexamethasone (Dxm), HCG and combined Dxm/HCG administration on the steroid levels was investigated in 8 adult male rhesus monkeys in November/December (= season of high testicular activity). Serum levels of cortisol, testosterone (T), androstenedione (A), 17-hydroxyprogesterone (17-OHP), oestradiol (Oe2), dehydroepiandrosterone (DHA) and its sulphate (DHA-S) were measured radioimmunologically.
Basal T and DHA serum levels were similar to those found in man, whilst A levels amounted to 1/10 and DHA-S to 1/30 of human values. Oe2 and 17-OHP levels were higher than in man. T and A levels were not suppressed by Dxm and very high degrees of stimulation were reached after HCG. The HCG-induced increment in T was smaller under Dxm/HCG than under HCG alone. 17-OHP levels were reduced to 25% of basal levels under Dxm, but the incremental response to Dxm/HCG was the same as that without Dxm. DHA and DHA-S were 60 and 75% lower under Dxm, and the increment in DHA after Dxm/HCG was less than that after HCG. DHA-S did not respond to HCG.
In conclusion, several important differences in basal steroid levels and in the response to dynamic testing were found in the rhesus monkey, as compared to man. The adrenal contributes 75% to circulating 17-OHP levels, and 60 and 75% respectively to DHA and DHA-S levels. A is produced solely by the testis in the rhesus monkey. As compared to man, the rhesus monkey testis in vivo synthesises T via both the Δ4 and Δ5 pathways. The Δ5 pathway appears to be dependent on adrenal steroid precursors.