bluuebunny/crossref_metadata_2025_split huggingface dataset:
“Todos somos familia aquí”: Familia, alianza y reproducción etnocultural entre los Calós (Gitanos) de Ciudad de México
Authors: Lagunas David 
Published: March 2024

ResumenEn este texto se analiza la relevancia del parentesco y la cultura familiar en la reproducción cultural de los Calós (Gitanos) que forman la comunidad de Ciudad de México. La metodología ha sido de orientación cualitativa empleando técnicas de inmersión antropológica. El objetivo es analizar cómo el sistema de parentesco se construye desde la genealogía como un constructo que permite la comparación cultural y corroborar el funcionamiento de una sociedad bilateral con un colorido patrilineal. Asimismo, se subraya la importancia de la preservación de la diferencia etnocultural, no solo a través de la organización social y familiar, sino a través de la endogamia sociobiológica. El hecho es que los Calós se sienten “un poco parientes entre sí”, aunque genealógicamente no lo sean. Esta lógica imprecisa de funcionamiento del sistema de parentesco plantea interesantes cuestiones al campo de los Romani Studies.
Vietnamese Buddhist encounters with South Asia in the 1950s
Authors: Gadkar-Wilcox Wynn 
Published: March 2024

AbstractThis article presents a comparison of two Vietnamese Buddhist monks who travelled to and spent time in South Asia in the 1950s. The first, Thích Tố Liên (1903–1977), travelled to Calcutta and then on to Sri Lanka in May 1950 to participate in the First General Conference of the World Fellowship of Buddhists. Though his encounter was relatively brief, it left a lasting impression. Tố Liên returned as an ardent advocate for the World Fellowship and for an internationalist view of Buddhism more generally. The second, Thích Minh Chàu (1918–2012), had a very different encounter with Sri Lanka and India. He spent most of the 1950s studying Pali manuscripts and earning his doctoral degree from the Nalanda Institute (then a part of the University of Bihar, now Nalanda University). During this time, he became an important popularizer of contemporary Indian ideas. While in South Asia, he contributed many articles to Buddhist journals back in Vietnam. He recounted his pilgrimage to major Buddhist sites, considered the contemporary influence of Buddhism in India, and analysed the works of everyone from Tagore to the Dalai Lama. This article will compare the South Asian experiences of these two Vietnamese Buddhist monks and analyse their impact on Buddhist unification and the Vietnamese Buddhist movement in the 1960s.
Phylogenetic evidence of a possible Trichuris globulosa species complex in Arabian camels from Kuwait
Authors: Henedi Adawia , Chan Abigail Hui En , Youssef Wessam , Taha Hoda A. , Thaenkham Urusa , Ashour Ameen A. 
Published: March 2024

AbstractDuring a 1-year study, Trichuris adults were obtained after necropsy of Arabian camels (Camelus dromedarius) from a slaughterhouse in Kuwait. Morphological and molecular identification was performed to confirm the identity of the Trichuris specimens obtained from C. dromedarius. Fifteen male Trichuris specimens were selected, and molecular identification was performed using mitochondrial cytochrome c oxidase subunit I, 12S ribosomal RNA, 16S ribosomal RNA genes and the nuclear internal transcribed spacer 2 (ITS2) region. Through phylogenetic analysis, 2 distinct groups were obtained using the mitochondrial genes, where group 1 showed a close relationship to Trichuris globulosa while group 2 showed a close relationship to Trichuris ovis, providing molecular evidence of a possible T. globulosa species complex. Additionally, the nuclear ITS2 region did not provide enough resolution to distinguish between the 2 groups of Trichuris specimens. Observation of morphological characters revealed variations in the shape of the male spicule sheath, where specimens present either a globular posteriorly truncated swelling or the absence of posteriorly truncated swelling. Moreover, the variations in male spicule sheath does not corroborate with the results of molecular data, suggesting the limited use of this character for identification of T. globulosa. In conclusion, molecular analysis suggests a possible species complex in T. globulosa, with the mitochondrial genetic markers successfully differentiating between the 2 groups. The limited use of the male spicule sheath as a diagnostic character for identification of T. globulosa is suggested.
Testing for proficiency effects and crosslinguistic influence in L2 processing: Filler-gap dependencies in L2 English by Jordanian-Arabic and Mandarin speakers
Authors: Al-Maani Alaa , Sloggett Shayne , Grillo Nino , Marsden Heather 
Published: March 2024

AbstractThis study expands on previous research into filler-gap dependency processing in second language (L2) English, by means of a replication of Canales’s (2012) self-paced reading study. Canales, among others, found that advanced L2-English speakers exhibited the same processing behavior that Stowe (1986) found for native English processing: On encountering a filler, they posited gaps in licensed positions and avoided positing gaps in grammatically unlicensed island positions. However, the previous L2 studies focused on advanced-level L2 proficiency and did not test specifically for first language (L1) influence. The present study compares two groups of intermediate-level L2-English speakers with contrasting non-wh-movement L1s, Jordanian Arabic and Mandarin, to investigate the effects of L1 influence and individual differences in proficiency. Our results provide evidence that at intermediate level, too, L2 filler-gap processing adheres to grammatical constraints. L1 did not affect this behavior, but proficiency effects emerged, with larger licensed filled-gap effects at higher proficiency.
Development of Flood Preparedness Behavior Scale: A Methodological Validity and Reliability Study
Authors: Osman Marwa , Taşdelen Teker Gülşen , Altıntaş Kerim Hakan 
Published: March 2024

AbstractBackground:Floods are the most frequent natural disasters with a significant share of their mortality. Preparedness is capable of decreasing the mortality of floods by at least 50%. This paper aims to present the psychometric properties of a scale developed to evaluate the behavior of preparedness to floods in Sudan and similar settings.Methods:In this methodological scale development study, experts assessed the content validity of the items of the developed scale. Data were collected from key persons of 413 households living in neighborhoods affected by the 2018 floods in Kassala City in Sudan. A pre-tested questionnaire of sociodemographic data and the Flood Preparedness Behavior Scale (FPBS) were distributed to the participants’ houses and recollected. Construct validity of the scale was checked using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). Internal consistency of the scale was checked using Cronbach’s alpha. Test-retest reliability was assessed by Pearson’s correlation coefficient. Item analyses and tests of significance of the difference in the mean scores of the highest and lowest score groups were carried out to ensure discriminatory power of the scale items.Results:Experts agreed on the scale items. Construct validity of the scale was achieved using EFA by removing 34 items and retaining 25 items that were structured in three factors, named as: measures to be done before, during, and after a flood. Confirmatory factor analysis confirmed the construct obtained by EFA. The loadings of the items on their factors in both EFA and CFA were all > 0.3 with significant associations and acceptable fit indices obtained from CFA. The three factors were found to be reliable in terms of internal consistency (Cronbach’s alpha coefficients for all factors were > 0.7) and test-retest reliability coefficient. In item analysis, the corrected total item correlations for all the items were > 0.3, and significant differences in the means of the highest and lowest score groups indicated good item discrimination power.Conclusion:The developed 25 items scale is an instrument which produces valid and reliable measures of preparedness behavior for floods in Sudan and similar settings.
Layered Mantle Flow Field Associated With Plate Kinematics and Slab Modulation Underneath the Horseshoe‐Shaped Banda Arc‐Islands
Authors: Li Sijia , Kong Fansheng , Liu Kelly H. , Gao Stephen S. 
Published: March 2024

AbstractThe Banda arc‐continent collision zone signifies one of the most seismically active and tectonically intricate zones. The high convergence rate across the region, coupled with the exceptionally arcuate arc and subducted slab, makes it an ideal locale for investigating interactions between plate (slab) kinematics and plastic flow in the asthenosphere, which can be diagnosed by seismic anisotropy from shear wave splitting analyses. In total, 206 pairs of splitting measurements using teleseismic SKS, SKKS, and PKS, along with 43 pairs using local S phases, are obtained by utilizing broadband seismic data from five permanent seismic stations. To reduce the ambiguity in determining the origin of anisotropy leading to the teleseismic splittings, which lack vertical resolution, crustal anisotropy is constrained according to the sinusoidal moveout of converted S phases at the Moho using receiver functions. A layered anisotropic structure based on joint analyses of the anisotropy measurements characterizing different depth layers suggests the presence of trench‐parallel flow both in the mantle wedge and the sub‐slab region. The northeastward motion of the slab, entrained by the fast‐moving Australian Plate, deflects asthenospheric materials. The modulation results in trench‐parallel plastic mantle flows and leads to the steepening of the southern portion of the asymmetric spoon‐shaped Banda slab. In the shallower part of the sub‐slab region, the northeastward Australian Plate motion produces simple shear in the transitional layer between the rigid lithosphere and the viscous asthenosphere. The shear deformation induces seismic anisotropy with resulting fast orientations in accordance with the plate motion direction.
Kappa-opioid receptor stimulation in the nucleus accumbens shell and ethanol drinking: Differential effects by rostro-caudal location and level of drinking
Authors: Pirino Breanne E. , Hawks Annie , Carpenter Brody A. , Candelas Pelagia G. , Gargiulo Andrew T. , Curtis Genevieve R. , Karkhanis Anushree N. , Barson Jessica R. 
Published: March 2024

AbstractAlthough the kappa-opioid receptor (KOR) and its endogenous ligand, dynorphin, are believed to be involved in ethanol drinking, evidence on the direction of their effects has been mixed. The nucleus accumbens (NAc) shell densely expresses KORs, but previous studies have not found KOR activation to influence ethanol drinking. Using microinjections into the NAc shell of male and female Long-Evans rats that drank under the intermittent-access procedure, we found that the KOR agonist, U50,488, had no effect on ethanol drinking when injected into the middle NAc shell, but that it promoted intake in males and high-drinking females in the caudal NAc shell and high-drinking females in the rostral shell, and decreased intake in males and low-drinking females in the rostral shell. Conversely, injection of the KOR antagonist, nor-binaltorphimine, stimulated ethanol drinking in low-drinking females when injected into the rostral NAc shell and decreased drinking in high-drinking females when injected into the caudal NAc shell. These effects of KOR activity were substance-specific, as U50,488 did not affect sucrose intake. Using quantitative real-time PCR, we found that baseline gene expression of the KOR was higher in the rostral compared to caudal NAc shell, but that this was upregulated in the rostral shell with a history of ethanol drinking. Our findings have important clinical implications, demonstrating that KOR stimulation in the NAc shell can affect ethanol drinking, but that this depends on NAc subregion, subject sex, and ethanol intake level, and suggesting that this may be due to differences in KOR expression.
Secondary use of genomic data: patients’ decisions at point of testing and perspectives to inform international data sharing
Authors: Martyn Melissa , Forbes Emily , Lee Ling , Kanga-Parabia Anaita , Weerasuriya Rona , Lynch Elly , Gleeson Penny , Gaff Clara 
Published: March 2024

AbstractInternational sharing of genomic data files arising from clinical testing of patients is essential to further improve genomic medicine. Whilst the general public are reluctant to donate DNA for research, the choices patients actually make about sharing their clinical genomic data for future re-use (research or clinical) are unknown. We ascertained the data-sharing choices of 1515 patients having genomic testing for inherited conditions or cancer treatment from clinical consent forms. To understand the experiences and preferences of these patients, surveys were administered after test consent (RR 73%). Almost all patients (98%) consented to share their data. Survey respondents’ decision recall was high (90%), but poorer if English was an additional language (p < 0.001). Parents deciding on behalf of children were over-represented amongst data-sharing decliners (p = 0.047) and decliners were more likely to believe that stored data could be easily reidentified (p < 0.001). A quarter of respondents did not know if reidentification would be easy and 44% of them were concerned about this possibility. Of those willing to share data overseas (60%), 23% indicated the recipient researcher’s country would affect their decision. Most respondents (89%) desired some ongoing control over research use of their data. Four preliminary data-sharing profiles emerged; their further development could inform tailored patient resources. Our results highlight considerations for establishment of systems to make clinical genomic data files available for reanalysis locally and across borders. Patients’ willingness to share their data – and value of the resulting research – should encourage clinical laboratories to consider sharing data systematically for secondary uses.
Transcriptome free energy can serve as a dynamic patient-specific biomarker in acute myeloid leukemia
Authors: Uechi Lisa , Vasudevan Swetha , Vilenski Daniela , Branciamore Sergio , Frankhouser David , O’Meally Denis , Meshinchi Soheil , Marcucci Guido , Kuo Ya-Huei , Rockne Russell , Kravchenko-Balasha Nataly 
Published: March 2024

Abstract
Acute myeloid leukemia (AML) is prevalent in both adult and pediatric patients. Despite advances in patient categorization, the heterogeneity of AML remains a challenge. Recent studies have explored the use of gene expression data to enhance AML diagnosis and prognosis, however, alternative approaches rooted in physics and chemistry may provide another level of insight into AML transformation. Utilizing publicly available databases, we analyze 884 human and mouse blood and bone marrow samples. We employ a personalized medicine strategy, combining state-transition theory and surprisal analysis, to assess the RNA transcriptome of individual patients. The transcriptome is transformed into physical parameters that represent each sample’s steady state and the free energy change (FEC) from that steady state, which is the state with the lowest free energy.We found the transcriptome steady state was invariant across normal and AML samples. FEC, representing active molecular processes, varied significantly between samples and was used to create patient-specific barcodes to characterize the biology of the disease. We discovered that AML samples that were in a transition state had the highest FEC. This disease state may be characterized as the most unstable and hence the most therapeutically targetable since a change in free energy is a thermodynamic requirement for disease progression. We also found that distinct sets of ongoing processes may be at the root of otherwise similar clinical phenotypes, implying that our integrated analysis of transcriptome profiles may facilitate a personalized medicine approach to cure AML and restore a steady state in each patient.
The evolutionary drivers and correlates of viral host jumps
Authors: Tan Cedric C. S. , van Dorp Lucy , Balloux Francois 
Published: March 2024

AbstractMost emerging and re-emerging infectious diseases stem from viruses that naturally circulate in non-human vertebrates. When these viruses cross over into humans, they can cause disease outbreaks, epidemics and pandemics. While zoonotic host jumps have been extensively studied from an ecological perspective, little attention has gone into characterizing the evolutionary drivers and correlates underlying these events. To address this gap, we harnessed the entirety of publicly available viral genomic data, employing a comprehensive suite of network and phylogenetic analyses to investigate the evolutionary mechanisms underpinning recent viral host jumps. Surprisingly, we find that humans are as much a source as a sink for viral spillover events, insofar as we infer more viral host jumps from humans to other animals than from animals to humans. Moreover, we demonstrate heightened evolution in viral lineages that involve putative host jumps. We further observe that the extent of adaptation associated with a host jump is lower for viruses with broader host ranges. Finally, we show that the genomic targets of natural selection associated with host jumps vary across different viral families, with either structural or auxiliary genes being the prime targets of selection. Collectively, our results illuminate some of the evolutionary drivers underlying viral host jumps that may contribute to mitigating viral threats across species boundaries.
Piezo1 regulates meningeal lymphatic vessel drainage and alleviates excessive CSF accumulation
Authors: Choi Dongwon , Park Eunkyung , Choi Joshua , Lu Renhao , Yu Jin Suh , Kim Chiyoon , Zhao Luping , Yu James , Nakashima Brandon , Lee Sunju , Singhal Dhruv , Scallan Joshua P. , Zhou Bin , Koh Chester J. , Lee Esak , Hong Young-Kwon 
Published: March 2024

AbstractPiezo1 regulates multiple aspects of the vascular system by converting mechanical signals generated by fluid flow into biological processes. Here, we find that Piezo1 is necessary for the proper development and function of meningeal lymphatic vessels and that activating Piezo1 through transgenic overexpression or treatment with the chemical agonist Yoda1 is sufficient to increase cerebrospinal fluid (CSF) outflow by improving lymphatic absorption and transport. The abnormal accumulation of CSF, which often leads to hydrocephalus and ventriculomegaly, currently lacks effective treatments. We discovered that meningeal lymphatics in mouse models of Down syndrome were incompletely developed and abnormally formed. Selective overexpression of Piezo1 in lymphatics or systemic administration of Yoda1 in mice with hydrocephalus or Down syndrome resulted in a notable decrease in pathological CSF accumulation, ventricular enlargement and other associated disease symptoms. Together, our study highlights the importance of Piezo1-mediated lymphatic mechanotransduction in maintaining brain fluid drainage and identifies Piezo1 as a promising therapeutic target for treating excessive CSF accumulation and ventricular enlargement.
Evaluating and optimizing Acid-pH and Direct Lysis RNA extraction for SARS-CoV-2 RNA detection in whole saliva
Authors: LaBute Brayden , Fong Jackie , Ziaee Farinaz , Gombar Robert , Stover Mathew , Beaudin Terry , Badalova Maria , Geng Qiudi , Corchis-Scott Ryland , Podadera Ana , Lago Kyle , Xu ZhenHuan , Lim Fievel , Chiu Felix , Fu Minghua , Nie Xiaofeng , Wu Yuanmin , Quan Corrina , Hamm Caroline , McKay R. Michael , Ng Kenneth , Porter Lisa A. , Tong Yufeng 
Published: March 2024

AbstractCOVID-19 has been a global public health and economic challenge. Screening for the SARS-CoV-2 virus has been a key part of disease mitigation while the world continues to move forward, and lessons learned will benefit disease detection beyond COVID-19. Saliva specimen collection offers a less invasive, time- and cost-effective alternative to standard nasopharyngeal swabs. We optimized two different methods of saliva sample processing for RT-qPCR testing. Two methods were optimized to provide two cost-efficient ways to do testing for a minimum of four samples by pooling in a 2.0 mL tube and decrease the need for more highly trained personnel. Acid-pH-based RNA extraction method can be done without the need for expensive kits. Direct Lysis is a quick one-step reaction that can be applied quickly. Our optimized Acid-pH and Direct Lysis protocols are reliable and reproducible, detecting the beta-2 microglobulin (B2M) mRNA in saliva as an internal control from 97 to 96.7% of samples, respectively. The cycle threshold (Ct) values forB2Mwere significantly higher in the Direct Lysis protocol than in the Acid-pH protocol. The limit of detection forN1gene was higher in Direct Lysis at ≤ 5 copies/μL than Acid-pH. Saliva samples collected over the course of several days from two COVID-positive individuals demonstrated Ct values for N1 that were consistently higher from Direct Lysis compared to Acid-pH. Collectively, this work supports that each of these techniques can be used to screen for SARS-CoV-2 in saliva for a cost-effective screening platform.
Identification of the shared hub gene signatures and molecular mechanisms between HIV-1 and pulmonary arterial hypertension
Authors: Mai Huanzhuo , Yang Xing , Xie Yulan , Zhou Jie , Wei Yiru , Luo Tingyan , Yang Jing , Cui Ping , Ye Li , Liang Hao , Huang Jiegang 
Published: March 2024

AbstractThe close link between HIV-1 infection and the occurrence of pulmonary arterial hypertension (PAH). However, the underlying molecular mechanisms of their interrelation remain unclear. The microarray data of HIV-1 and PAH were downloaded from GEO database. We utilized WGCNA to identify shared genes between HIV-1 and PAH, followed by conducting GO and pathway enrichment analyses. Subsequently, differentially genes analysis was performed using external validation datasets to further filter hub genes. Immunoinfiltration analysis was performed using CIBERSORT. Finally, hub gene expression was validated using scRNA-seq data. We identified 109 shared genes through WGCNA, primarily enriched in type I interferon (IFN) pathways. By taking the intersection of WGCNA important module genes and DEGs, ISG15 and IFI27 were identified as pivotal hub genes. Immunoinfiltration analysis and scRNA-seq results indicated the significant role of monocytes in the shared molecular mechanisms of HIV-1 and PAH. In summary, our study illustrated the possible mechanism of PAH secondary to HIV-1 and showed that the heightened IFN response in HIV-1 might be a crucial susceptibility factor for PAH, with monocytes being pivotal cells involved in the type I IFN response pathway. This provides potential new insights for further investigating the molecular mechanisms connecting HIV-1 and PAH.