bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of a Cancer Stem Cell related Histone Acetylation Regulatory Genes Prognostic Model for Hepatocellular Carcinoma via Bioinformatics Analysis: Implications for Tumor Chemotherapy and Immunity
Authors: Dai Qian , Zhu Jie , Yang Jing , Zhang Chun-Yan , Yang Wen-Jing , Pan Bai-Shen , Yang Xin-Rong , Guo Wei , Wang Bei-li 
Published: April 2024

Background:
Cancer stem cells (CSC) play an important role in the development of
Liver Hepatocellular Carcinoma (LIHC). However, the regulatory mechanisms between acetylation-
associated genes (HAGs) and liver cancer stem cells remain unclear.

Objective:
To identify a set of histone acetylation genes (HAGs) with close associations to liver
cancer stem cells (LCSCs), and to construct a prognostic model that facilitates more accurate prognosis
assessments for LIHC patients.

Methods:
LIHC expression data were downloaded from the public databases. Using mRNA expression-
based stemness indices (mRNAsi) inferred by One-Class Logistic Regression (OCLR),
Differentially Expressed Genes (DEGs) (mRNAsi-High VS. mRNAsi-Low groups) were intersected
with DEGs (LIHC VS. normal samples), as well as histone acetylation-associated genes
(HAGs), to obtain mRNAsi-HAGs. A risk model was constructed employing the prognostic
genes, which were acquired through univariate Cox and Least Shrinkage and Selection Operator
(LASSO) regression analyses. Subsequently, independent prognostic factors were identified via
univariate and multivariate Cox regression analyses and then a nomogram for prediction of LIHC
survival was developed. Additionally, immune infiltration and drug sensitivity analysis were performed
to explore the relationships between prognostic genes and immune cells. Finally, the expressions
of selected mRNAsi-HAGs were validated in the LIHC tumor sphere by quantitative Reverse
Transcription Polymerase Chain Reaction (qRT-PCR) assay and western blot analysis.

Results:
Among 13 identified mRNAsi-HAGs, 3 prognostic genes (HDAC1, HDAC11, and
HAT1) were selected to construct a risk model (mRNAsi-HAGs risk score = 0.02 * HDAC1 +
0.09 * HAT1 + 0.05 * HDAC11). T-stage, mRNAsi, and mRNAsi-HAGs risk scores were identified
as independent prognostic factors to construct the nomogram, which was proved to predict
the survival probability of LIHC patients effectively. We subsequently observed strongly positive
correlations between mRNAsi-HAGs risk score and tumor-infiltrating T cells, B cells and
macrophages/monocytes. Moreover, we found 8 drugs (Mitomycin C, IPA 3, FTI 277,
Bleomycin, Tipifarnib, GSK 650394, AICAR and EHT 1864) had significant correlations with
mRNAsi-HAGs risk scores. The expression of HDAC1 and HDAC11 was higher in CSC-like
cells in the tumor sphere.

Conclusion:
This study constructed a mRNAsi and HAGs-related prognostic model, which has
implications for potential immunotherapy and drug treatment of LIHC.
The Impact of Digital Self-Monitoring of Weight on Improving Diabetes Clinical Outcomes: Quasi-Randomized Study
Authors: Fundoiano-Hershcovitz ‪Yifat , Ritholz Marilyn D , Horwitz David L , Behar Ephraim , Manejwala Omar , Goldstein Pavel 
Published: April 2024

Background
The management of type 2 diabetes (T2D) and obesity, particularly in the context of self-monitoring, remains a critical challenge in health care. As nearly 80% to 90% of patients with T2D have overweight or obesity, there is a compelling need for interventions that can effectively manage both conditions simultaneously. One of the goals in managing chronic conditions is to increase awareness and generate behavioral change to improve outcomes in diabetes and related comorbidities, such as overweight or obesity. There is a lack of real-life evidence to test the impact of self-monitoring of weight on glycemic outcomes and its underlying mechanisms.

Objective
This study aims to assess the efficacy of digital self-monitoring of weight on blood glucose (BG) levels during diabetes management, investigating whether the weight changes may drive glucose fluctuations.

Methods
In this retrospective, real-world quasi-randomized study, 50% of the individuals who regularly used the weight monitoring (WM) feature were propensity score matched with 50% of the users who did not use the weight monitoring feature (NWM) based on demographic and clinical characteristics. All the patients were diagnosed with T2D and tracked their BG levels. We analyzed monthly aggregated data 6 months before and after starting their weight monitoring. A piecewise mixed model was used for analyzing the time trajectories of BG and weight as well as exploring the disaggregation effect of between- and within-patient lagged effects of weight on BG.

Results
The WM group exhibited a significant reduction in BG levels post intervention (P<.001), whereas the nonmonitoring group showed no significant changes (P=.59), and both groups showed no differences in BG pattern before the intervention (P=.59). Furthermore, the WM group achieved a meaningful decrease in BMI (P<.001). Finally, both within-patient (P<.001) and between-patient (P=.008) weight variability was positively associated with BG levels. However, 1-month lagged back BMI was not associated with BG levels (P=.36).

Conclusions
This study highlights the substantial benefits of self-monitoring of weight in managing BG levels in patients with diabetes, facilitated by a digital health platform, and advocates for the integration of digital self-monitoring tools in chronic disease management. We also provide initial evidence of testing the underlying mechanisms associated with BG management, underscoring the potential role of patient empowerment.
Reconnecting and Reconstructing: Taking Stock of the STLHE 2022 conference
Authors: Miller-Young Janice , Chan Eric 
Published: April 2024

Academic conferences are key spaces of knowledge sharing and professional development. As a national society which has pledged to undergo “a robust examination of its attention to equity, diversity, and inclusion throughout the organization” (STLHE/SAPES 2021), we undertook an evaluation of the diversity of participants and presentation topics at the 2022 conference. This paper reports on some of the distinctive aspects of the 2022 conference as well as the findings of the evaluation such as diversity of participants by gender and ethnic identity, province, institution type, discipline, and more. While the 2022 conference was unique in many ways due to being STLHE’s first face-to-face conference since 2019, this report provides a useful baseline with which to compare the diversity of participants and presentation topics in future years. The report concludes with additional recommendations for programming decisions to support and evaluate equity, diversity, and inclusivity at future conferences.
Keywords: conference, diversity, participation, presentation topics, Society for Teaching and Learning in Higher Education
Gordon Peter Wilson (1946–2020): Teacher, Mentor, Scholar, and Friend
Authors: Moehrle Stephen R. , Persson Martin E. 
Published: April 2024

SYNOPSIS
G. Peter Wilson, most recently on the faculty at Boston College, was a consummate academic and leader. He profoundly influenced accounting research and the art and science of accounting instruction. He also advanced the accounting academy through his various leadership roles at the American Accounting Association. This memorial article documents Wilson’s early upbringing, academic career, professional activities, and achievements. The overarching themes are love and impact. Wilson loved life, loved his work, and above all, loved his family, friends, students, and colleagues. As a result, he profoundly impacted each aspect of the academy—research, teaching, and service. Indeed, his impact continues through the undergraduate and master students he inspired, the doctoral students he mentored at various universities and consortia, the colleagues he collaborated with, and the many who continue to learn from his innovations. This article sets forth the most significant impacts to preserve the past and inspire future generations.
JEL Classifications: M41; M42; M48.
Do Auditors View Off-the-Clock Misbehavior by Company Leadership as a Signal of Tone at the Top?
Authors: Christensen Brant E. , Cline Brandon N. , Lundstrom Nathan G. , Yore Adam S. 
Published: April 2024

ABSTRACT
We study off-the-clock indiscretion accusations against corporate officers and directors and examine the extent, effectiveness, and context of auditors’ response. In the year that indiscretion allegations are first publicized, auditors charge higher fees and are more likely to resign. Auditors respond to allegations against both top executives and board members. Further, reactions are strongest when allegations demonstrate a lack of individual integrity and, separately, when the audit office has previously audited other similarly accused clients. Importantly, the resulting increase in auditors’ effort partially negates the association between indiscretions and lower financial reporting quality. However, auditors are primarily reactive, rather than proactive, and their response is stronger when the accused client is less important economically. These results suggest that company leadership’s off-the-clock indiscretions are signals to auditors of poor tone at the top, but the audit response is not uniform across all clients.
JEL Classifications: M41; M42; M48; G34.
IMPLEMENTASI WAZUH MENGGUNAKAN METODE PPDIOO DI SISTEM KEAMANAN JARINGAN PSDKU UNIVERSITAS LAMPUNG WAYKANAN SEBAGAI DETEKSI DAN RESPON SERANGAN SIBER
Authors: Shafiyyah Azzah , Nama Gigih Forda , Pradipta Rio Ariestia 
Published: April 2024

PSDKU Universitas Lampung Way Kanan membutuhkan sistem keamanan jaringan yang efektif untuk melindungi jaringan komputer server lab komputer dari serangan siber yaitu brute force dan Denial of Service (DoS)Untuk meningkatkan keamanan jaringan, diperlukan implementasi sistem keamanan jaringan dengan menggunakan platform wazuh sebagai server monitoring dan wazuh agent yang diinstal pada komputer server jaringan lab komputer PSDKU menggunakan Server Utama PSDKU. Metodepengembangan sistem keamanan yang digunakan yaitu PPDIOO (prepare, plan, design, implement, operate, optimize ) cisco. Dalam sistem ini, semuaaktivitas dari wazuh agent akan dikirimkan ke wazuh server untuk dilakukan pemantauan dan deteksi serangan siber. Kemudian dilakukan simulasi seranganbrute force dan Denial of Service (DoS) terhadap wazuh agent yang terinstal pada server jaringan lab komputer. Respon dari serangan bruteforce yang akan dilakukan adalah dengan memblokir alamat IP penyerang menggunakan software fail2ban dan serangan DoS dengan Active Response Wazuh sehingga penyerang tidak dapat mengakses dan tidak dapat terhubung ke komputer server jaringan lab komputer PSDKU. Sistem keamanan jaringan tidak dapatmendeteksi serangan DoS dengan jumlah rata-rata koneksi dibawah 4000 koneksi http, dan Serangan DoS berhasil dilakukan dengan hasil yang menunjukkan bahwa Server Utama PSDKU memiliki ketahanan dalammenerima dan melayani koneksi HTTP dibawah 3.522.013 koneksi. Sehinggasistem keamanan jaringan dapat ditingkatkan dan respon terhadap serangan siber dapat dilakukan secara cepat dan efektif.
Sistem Kontrol Color Sorting Machine Dengan Pengolahan Citra Digital
Authors: Chairi Al , Mukhaiyar Riki 
Published: April 2024

Penelitian ini bertujuan untuk membuat sorting machine otomatis berbasis digital image processing. Digital image processing pada penelitian ini digunakan sebagai sistem deteksi objek benda berwarna yang berbentuk tabung dengan diamater 40 mm dan tinggi 25 mm dengan berat ± 28 gram dari bahan High Density Polyethylene (HDPE) yang berjalan di sebuah mesin sorter berupa belt conveyor. Image processing di proses menggunakan personal computer (PC) dengan bahasa pemograman Python menggunakan pustaka open computer vision (openCV). Metode pendeteksian objek pada penelitian ini menggunakan metode color filtering model ruang warna HSV agar dapat mendeteksi objek yang dibatasi terhadap tiga warna yaitu warna merah, biru dan kuning. Model ruang warna HSV (Hue, Saturation, Value) dipilih karena ruang warna ini telah memperhitungkan kemurnian warna dan intensitas cahaya. Setpoint untuk nilai HSV diatur di Graphical User Interface tracbar GUI-Python yang dibuat menggunakan pustaka tkinter. Setpoint nilai HSV masing – masing warna disimpan dalam JavaScript Object Notation (JSON). Hasil dari data Image processing yang di proses di personal computer akan di kirim ke Mikrokontroler ATmega 2560 dengan komunikasi serial USB. Mikrokontroler ATmega 2560 sendiri berfungsi sebagai pusat kendali komponen yang terdapat pada sorting machine. Hasil penelitian ini menunjukkan image processing bisa mendeteksi objek berwarna dengan baik tetapi setpoint nilai HSV yang harus di setting sesuai dengan pencahayaan ruangan.
Desain dan Implementasi Modul Konverter DC-DC Buck Boost Dengan Pengendali PID
Authors: Kunigar Regiantoro , Tahtawi Adnan Rafi Al , Ilman Sofyan Muhammad 
Published: April 2024

Konverter DC-DC merupakan salah satu perangkat elektronika daya yang berperan penting dalam berbagai aplikasi sistem energi terbarukan, industri, maupun bidang telekomunikasi. Buck-boost converter merupakan salah satu jenis DC-DC converter yang mampu menurunkan dan menaikan tegangan DC dengan pengaturan modulasi lebar pulsa. Namun, saat ini modul buck-boost converter yang memiliki fitur kendali closed-loop masih jarang ditemukan. Tujuan dari penelitian ini adalah merancang dan mengimplementasikan modul Buck Boost converter dengan dilengkapi kendali PID sebagai pengendali tegangan keluarannya. Modul dirancang sedemikian rupa agar mudah dipergunakan dan dilengkapi fitur kendali closed-loop. Metode kendali PID dirancang menggunakan metode tuning Ziegler Nichols tipe I untuk mencari parameter PID agar tegangan keluaran yang dihasilkan sesuai yang diinginkan. Metode ini digunakan karena memiliki kemudahan dan menjadi metode dasar dalam pengendalian sistem dinamik seperti buck-boost converter. Hasil yang diperoleh dari penelitian ini adalah berupa modul konverter dengan pengendalian tegangan yang menghasilkan error steady-state pada mode operasi kerja boost dan buck sekitar 0,4% dan settling time sekitar 2 s meskipun masih ditemukan osilasi saat kondisi steady-state.
Monitoring Pembangkit Listrik Tenaga Angin Menggunakan Human Machine Interface
Authors: Fajri Salihul , Yuhendri Muldi 
Published: April 2024

Pembangkit listrik tenaga angin adalah salah satu jenis energi terbarukan yang mulai berkembang di Indonesia. Pembangkit listrik tenaga angin ini menggunakan turbin angin untuk mengkonversikan daya angin menjadi daya mekanik yang akan menggerakan generator listrik. Generator yang digunakan pada pembangkit listrik tenaga angin ini memiliki batas maksimum. Turbin angin yang beroperasi harus dibawah batas maksimum generator agar tidak terjadi kerusakan pada generator tersebut. Agar tidak terjadi operasi melampaui batas maksimum generator, maka perlu dilakukan monitoring dan proteksi terhadap komponen-komponen yang ada pada pembangkit listrik tenaga angin tersebut. Dengan hal itu paper ini mengusulkan monitoring pembangkit listrik tenaga angin menggunakan Human Machine Interface (HMI) yang dilengkapi dengan Programable Logic Controller (PLC). Alat yang dibuat dalam penelitian ini diujicobakan pada pembangkit listrik tenaga angin yang menggunakan turbin angin sumbu horizontal dan generator sinkron jenis magnet permanent. Monitoring pembangkit listrik tenaga angin ini dirancang dengan menggunakan Programable Logic Controller (PLC) S7 1200 1215C DC/DC/Relay dan Human Machine Interface (HMI) Simatic KTP 700 Basic PN untuk display dari parameter yang dimonitor yaitu arus, tegangan, kecepatan angin, kecepatan putaran dan daya. Monitoring ini dilengkapi dengan sensor tegangan, sensor arus, sensor kecepatan angin, sensor kecepatan putaran dan kontaktor sebagai pemutus arus ketika telah melebihi batas arus yang telat diatur sebelumnya. HMI sudah berhasil menampilkan data tegangan, arus kecepatan angin, kecepatan putaran dan daya. Hasil eksperimen menunjukan bahwa monitoring pembangkit listrik tenaga angin yang diusulkan telah bekerja dengan baik sesuai dengan tujuan.
Rancang Bangun Lampu Pengatur Lalu Lintas Berbasiskan Gerbang Logika
Authors: Mirshad Emilham , Diputra Yudhi , Mulya Rudi 
Published: April 2024

Sistem kendali lampu lalu lintas (Lampu lalu lintas ) mengambil peran penting dalam memberikan pengaturan yang lebih baik dan mengontrol arus lalu lintas. Strategi yang lebih baik dalam pengendalian arus lalu lintas dapat meningkatkan pergerakan kendaraan dengan mempersingkat waktu perjalanan. Penulisan ini bertujuan untuk merancang dan membuat alat Lampu lalu lintas dengan gerbang logika. Perancangan peralatan menggunakan sistem tata lampu lalu lintas simpang empat fase yang berarti akan ada empat lampu hijau yang akan menyala secara bergantian dan empat lampu merah yang 3 hidup bersamaan. Tahap desain dimulai dari perancangan perangkat keras yang mengacu pada sistem blok diagram, selanjutnya program dibuat sesuai dengan perencanaan. Sensor Infra merah adalah komponen elektronika yang dapat mengidentifikasi cahaya infra merah (infra red, IR). Sensor infra merah atau detektor infra merah saat ini ada yang dibuat khusus dalam satu modul dan dinamakan sebagai IR Detector Photomodules. IR Detector Photomodules merupakan sebuah chip detektor infra merah digital yang di dalamnya terdapat fotodiode dan penguat (amplifier). Hasil pengujian alat ini menunjukkan LED dan seven segment yang ditampilkan akan menyala sesuai masukan sensor
Scoping review of the use of mesenchymal stem and stromal cell products in cats, Part 1: current logistics and safety
Authors: Webb Tracy L. , Webb Craig B. 
Published: April 2024

Abstract
Despite a pressing need for new therapies to address unmet veterinary medical need, no approved stem cell products are available for use in cats in the US. To evaluate the current state of mesenchymal stem or stromal cell (MSC) research in cats, a scoping review of published literature was performed, which identified 108 publications related to feline MSCs. Twenty-six of the articles described administration of MSC products to a total of 215 cats. Twelve of the studies included a control group. These experimental and clinical trials used 7 cell sources, 9 administration routes, 12 delivery vehicles, and a 300-fold range in dosages for initial studies in healthy cats and cats with 12 naturally occurring and induced diseases. The majority of studies administered 2 doses of allogeneic, adipose-derived MSC IV and monitored a median of 6.5 treated cats for a median of 90 days. The majority (150/215 [69.8%]) of cats had no reported adverse events associated with treatment. Although an increase in feline MSC publications in the past 10 years indicates progress, the wide variety and small number of studies using MSCs and MSC products in cats demonstrates that current evaluations are mostly still in the discovery phase, and several issues remain related to larger scale trials using MSC products in cats. The current available publications provide information to direct further clinical study development and informed owner consent for study enrollment.
Relationships of apolipoprotein E genotypes with a cluster of seven in persons with type 2 diabetes
Authors: Barre Douglas E. , Mizier-Barre Kazimiera A. , Griscti Odette , Hafez Kevin 
Published: April 2024

Abstract

Objective.
The objective of the study was to determine if there would be statistically significant differences or trends among apolipoprotein E genotypes (2/2, 2/3, 2/4, 3/3, 3/4, and 4/4) for each member of the cluster of seven associated with type 2 diabetes (T2D). The cluster of seven includes abdominal obesity, hypertension, platelet hyperaggregability, hyperglycemia, dyslipidemia (decreased plasma levels of high-density lipoprotein cholesterol (HDL-C) and increased plasma levels of triglycerides)), increased low-density lipoprotein (LDL) oxidation, and increased inflammation.

Methods.
Forty-six patients with well-controlled T2D participated in the study. Abdominal obesity (assessed by waist circumference), hypertension (measured by manual sphygmomanometry), platelet hyperaggregability (measured by bleeding time), hyperglycemia (by enzymatic kit and spectrophotometry), decreased plasma levels of HDL-C and increased plasma levels of triglycerides (by enzymatic kit and spectrophotometry), increased LDL oxidation (measured by LDL conjugated dienes using spectrophotometry) and increased inflammation measured by C-reactive protein (CRP) (by EIA kit) were determined.

Results.
All genotypes, except 2/2 were found in the population studied. Abdominal obesity did not vary significantly across the five genotypes. However, glucose levels trended progressively higher going from 2/3 to 2/4 to 3/4 to 4/4. Systolic blood pressure was higher in 3/4 compared to 2/4 and trended higher in 3/4 compared to 3/3. Diastolic blood pressure trended higher in 3/3 vs 2/4 and significantly higher in 3/4 compared to 2/4. Triglycerides trended higher in 3/4 vs 3/3 while HDL-C came close to trending downward in 4/4 compared to 2/4. Bleeding time was unaffected by genotype. Plasma LDL conjugated dienes trended higher in 3/4 vs 2/4 and were significantly higher in 3/4 vs 3/3. CRP trended higher in 4/4 vs 2/3.

Conclusion.
We can conclude that those with at least one 4 allele in the presence of another allele being 2, 3 or 4 is potentially (in the case of trends) deleterious or is deleterious in terms of hyperglycemia, hypertension (systolic and diastolic blood pressure), dyslipidemia, LDL conjugated dienes and CRP levels.
ERN1 knockdown modifies the hypoxic regulation of homeobox gene expression in U87MG glioblastoma cells
Authors: Krasnytska Daria A. , Khita Olena O. , Viletska Yuliia M. , Minchenko Dmytro O. , Halkin Oleh V. , Rudnytska Olha V. , Hoian Sofiia L. , Minchenko Oleksandr H. 
Published: April 2024

Abstract

Objective.
Homeobox genes play an important role in health and disease including oncogenesis. The present investigation aimed to study ERN1-dependent hypoxic regulation of the expression of genes encoding homeobox proteins MEIS (zinc finger E-box binding homeobox 2) and LIM homeobox 1 family, SPAG4 (sperm associated antigen 4) and NKX3-1 (NK3 homeobox 1) in U87MG glioblastoma cells in response to inhibition of ERN1 (endoplasmic reticulum to nucleus signaling 1) for evaluation of their possible significance in the control of glioblastoma growth.

Methods.
The expression level of homeobox genes was studied in control (transfected by vector) and ERN1 knockdown U87MG glioblastoma cells under hypoxia induced by dimethyloxalylglycine (0.5 mM for 4 h) by quantitative polymerase chain reaction and normalized to ACTB.

Results.
It was found that hypoxia down-regulated the expression level of LHX2, LHX6, MEIS2, and NKX3-1 genes but up-regulated the expression level of MEIS1, LHX1, MEIS3, and SPAG4 genes in control glioblastoma cells. At the same time, ERN1 knockdown of glioblastoma cells significantly modified the sensitivity of all studied genes to a hypoxic condition. Thus, ERN1 knockdown of glioblastoma cells removed the effect of hypoxia on the expression of MEIS1 and LHX1 genes, but increased the sensitivity of MEIS2, LHX2, and LHX6 genes to hypoxia. However, the expression of MEIS3, NKX3-1, and SPAG4 genes had decreased sensitivity to hypoxia in ERN1 knockdown glioblastoma cells. Moreover, more pronounced changes under the conditions of ERN1 inhibition were detected for the pro-oncogenic gene SPAG4.

Conclusion.
The results of the present study demonstrate that hypoxia affected the expression of homeobox genes MEIS1, MEIS2, MEIS3, LHX1, LHX2, LHX6, SPAG4, and NKX3-1 in U87MG glioblastoma cells in gene-specific manner and that the sensitivity of all studied genes to hypoxia condition is mediated by ERN1, the major pathway of the endoplasmic reticulum stress signaling, and possibly contributed to the control of glioblastoma growth. A fundamentally new results of this work is the establishment of the fact regarding the dependence of hypoxic regulation of SPAG4 gene expression on ER stress, in particular ERN1, which is associated with suppression of cell proliferation and tumor growth.