bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of protein levels on production performance, nutritional values, and phase feeding of two-spotted cricket
Authors: Kaewtapee Chanwit , Triwai Punsak , Inson Chama , Masmeatathip Roungthip , Sriwongras Piyapong 
Published: March 2024

Abstract
Two experiments were conducted to determine the effects of different protein levels on the growth performance, feed efficiency and nutritional values, and phase feeding of the 2-spotted cricket (Gryllus bimaculatus de Geer). In experiment 1, 4 crude protein (CP) diets were formulated to contain 18%, 20%, 22%, or 24% CP, respectively. A sample of 7-day-old 3,600 crickets was equally divided into 24 plastic boxes (150 crickets each) in a completely randomized design with 4 diets and 6 replications. In experiment 2, 2-phase feedings were used. For starting period (days 7–18), crickets in all treatments were fed a diet containing 22% CP. During the growing period (days 19–35), 3 groups of crickets were fed diets containing 18%, 20%, and 22% CP. In the overall period of experiment 1, the crickets fed with 22% CP diet had greater body weight compared to those fed with 18% CP diet. In addition, the crickets fed with 22% CP diet had the lowest feed conversion ratio (FCR). The broken-line model indicated the growth pattern changed on day 18. In experiment 2, the crickets fed with 20% CP diet from days 19 to 35 had greater growth performance and lower FCR than those fed with 18% CP, but not different from those fed with 22% CP. In conclusion, 22% CP can increase growth performance by improving the feed efficiency of crickets. The implementation of 2-phase feedings using 20% CP, during the growing period, could be considered as a cost-effective strategy for sustainable cricket production.
Redating Barnabas
Authors: Finnis John 
Published: March 2024

Abstract
The undisturbed consensus that three verses in Barnabas (Bn) 16 refer to the Temple’s destruction by Titus, and to a rumoured rebuilding sometime in the subsequent 65 years, neglects to ask whether those verses would make good sense to readers in the generation before Titus. They would, and as references to the destruction by Nebuchadnezzar, and reconstruction by Zerubbabel, vassal of Cyrus, would cohere much better with the preceding 14 chapters. And since the intertextual ‘echoes’ between Bn and Pauline letters (and 1 Peter and Hebrews) are all more plausible when reversed, and the 11th ‘king’ in Bn 4 may be Agrippa I rather than Vespasian, Nerva, or Hadrian, we can look to a date c.40. Then Bn’s linguistic and thematic links with Acts’ accounts of Stephen’s Speech and of Barnabas in Syrian Antioch are best explained if Bn was written by Barnabas to his Antioch congregation, when fetching Paul to Antioch. The classic but long-doubted objections to Barnaban authorship weigh even less if Bn was thus a generation earlier than they assumed. Finally, the sequence of the earliest witnesses to the Christian Two Ways is clarified.
A Case of Multidrug-Resistant Tuberculosis in an Active Duty Military Health Care Worker
Authors: Saunders Amanda E , Shanahan Kevin M , Downs John W 
Published: March 2024

ABSTRACT
Cases of active tuberculosis (TB) in the U.S. Military have fallen over the last century in large part due to improved screening and treatment options. The subset of multidrug-resistant TB (MDR-TB) is almost nonexistent within the U.S. Military. We present a case of MDR-TB in an active duty U.S. Military health care worker and discuss several considerations for treatment that may present challenges for U.S. Military medical practitioners. A 30-year-old active duty Soldier was referred to Army public health services after a bronchoscopy sample was positive for Mycobacterium tuberculosis complex. Sputum smears were negative for acid-fast bacilli, suggesting lower risk for community spread. One month after initiation of the standard 4-drug regimen for active TB, genetic susceptibility testing found the patient’s M. tuberculosis isolate to be resistant to rifampin, isoniazid, and pyrazinamide. Contact investigation efforts among co-workers and family members fortunately found no new interferon-gamma release assay conversions. Coordination of public health assets to ensure a successful treatment regimen occurred across varied local, state, and federal agencies. Atypical medications required coordination with the FDA for procurement. An extensive surveillance plan for medication adverse effects was required. Finally, questions of public health authority versus patient autonomy arose requiring multidisciplinary input and ethical discussions.
Lessons Learned: Large-Scale Perfused Cadaver Training in Three Different Curricular Environments
Authors: Koo Alex Y , Rodgers David K , Hohman Marc H , Muise Jason R , Couperus Kyle S , Phelps Jillian F 
Published: March 2024

ABSTRACT

Background
Perfused cadavers are viable training models for operating room surgical skills, increasing fidelity of vascular anatomy, dissection, and tissue handling. In addition, perfused cadavers may have benefits in military medical training environments with a focus on hemorrhage control and vascular access.

Objectives
We created a large-scale training exercise with perfused cadavers in three different environments and aim to share the curricular design and feedback from the exercise.

Methods
We conducted a 4-day simulation training exercise with 13 perfused cadavers in 3 different environments: hospital environment, the austere echelons of care environment, and a controlled-settings tent for hemorrhage control and needle decompression training. Through an anonymous online survey and an after-action review (AAR), we elicited feedback on advantages, disadvantages, costs, and comparison to models of porcine and fresh cadavers for procedures and existing courses.

Results
A total of 324 participants were trained with 13 perfused cadavers for over 4 days from a variety of specialties and on different procedures and surgical skills. Based on 130 respondents to the survey and the AAR, perfused cadavers were rated to have realistic arterial bleeding and bleeding control training. In addition, perfused cadavers provided increased realism and fidelity for surgical skills. There were disadvantages of logistical requirements, cleanup, costs, and mobility.

Conclusion
Perfused cadavers can be implemented effectively for hemorrhage control training, surgical and procedure skills, and even exercises in austere environments, but require significant logistical and planning considerations. The training value is maximized with a progressive curriculum from hemorrhage control iterations to more invasive surgical procedures, such as thoracotomy and exploratory laparotomy.
Addressing Differences in Knowledge and Experience in Trauma Care Capabilities Among an International Team of Military Medical Care Providers in a Deployed Setting
Authors: Holvelinsky Sarah , Travers Taylor , Stierwalt Joshua B , Schunk Paul , Murray Brian Patrick 
Published: March 2024

ABSTRACT
Military medical personnel are crucial in providing life-saving care at the point of injury (POI) in challenging environments such as combat zones and disaster areas. This article examines the specialized training US Military medical personnel undergo before deployment and the increasing trend of deploying as part of multinational forces in operations like those in Afghanistan with NATO and non-NATO countries. Integrating medical teams from diverse backgrounds poses significant challenges in maintaining a cohesive and efficient team due to varying trauma management training standards and medical practices among the allied forces. Tactical Combat Casualty Care (TCCC) training and the development of clinical practice guidelines (CPG) by the US Joint Trauma Service have been key strategies by the US Military to standardize care. However, the variation in trauma management training among NATO allies and the differences in medical subspecialties and approaches can lead to inefficiencies and reduced effectiveness in a multinational trauma center setting. For instance, the approach to trauma care can significantly differ between the US and European countries, impacting the interoperability and teamwork in multinational medical teams. To address these challenges, the article highlights the importance of standardized medical training programs that include cultural awareness to enhance the effectiveness of multidisciplinary, multinational medical teams. It also underscores the necessity for standardized international trauma training in the face of increasing global conflicts and the potential for large-scale combat operations. The article discusses the “Trauma Tuesday” program implemented in Kabul, Afghanistan, as an example of how intensive training and simulation exercises can improve team dynamics, knowledge, and skills in trauma management among a diverse team from various nations. The need for ongoing education and developing a standard for managing trauma patients in international teams is emphasized to ensure effective communication and coordination. The article suggests that multinational trauma training can significantly improve team cohesion and critical life-saving skills, essential for future battlefields where access to definitive care may be delayed. Further research is recommended to explore the best methods for achieving effective multinational medical team integration and training standardization.
The diversity of splicing modifiers acting on A-1 bulged 5′-splice sites reveals rules for rational drug design
Authors: Malard Florian , Wolter Antje C , Marquevielle Julien , Morvan Estelle , Ecoutin Agathe , Rüdisser Simon H , Allain Frédéric H T , Campagne Sebastien 
Published: March 2024

Abstract
Pharmacological modulation of RNA splicing by small molecules is an emerging facet of drug discovery. In this context, the SMN2 splicing modifier SMN-C5 was used as a prototype to understand the mode of action of small molecule splicing modifiers and propose the concept of 5′-splice site bulge repair. In this study, we combined in vitro binding assays and structure determination by NMR spectroscopy to identify the binding modes of four other small molecule splicing modifiers that switch the splicing of either the SMN2 or the HTT gene. Here, we determined the solution structures of risdiplam, branaplam, SMN-CX and SMN-CY bound to the intermolecular RNA helix epitope containing an unpaired adenine within the G-2A-1G+1U+2 motif of the 5′-splice site. Despite notable differences in their scaffolds, risdiplam, SMN-CX, SMN-CY and branaplam contact the RNA epitope similarly to SMN-C5, suggesting that the 5′-splice site bulge repair mechanism can be generalised. These findings not only deepen our understanding of the chemical diversity of splicing modifiers that target A-1 bulged 5′-splice sites, but also identify common pharmacophores required for modulating 5′-splice site selection with small molecules.
Pulsed operation of perovskite LEDs: a study on the role of mobile ions
Authors: Torre Cachafeiro Miguel A , Kumawat Naresh Kumar , Gao Feng , Tress Wolfgang 
Published: March 2024

ABSTRACT
Metal halide perovskite light-emitting diodes (PeLEDs) are a promising technology for energy-efficient and cost-effective lighting and displays, thanks to their tunable color emission, high brightness, color purity and low-temperature fabrication. However, the mixed ionic-electronic conductivity of perovskite materials presents unique challenges, as ionic defects can redistribute under operation, affecting the energy landscape and charge recombination mechanisms. Our drift-diffusion simulations establish a connection between the transient electroluminescence (TrEL) signals of PeLEDs under pulsed operation and the influence of mobile ions. We find that the TrEL plateau value’s dependence on the duty cycle and end-of-pulse overshoot can be explained by the time-varying distribution of ionic defects. The inclusion of mobile ions is crucial to understand the TrEL response. Moreover, the simulations highlight injection barriers at the perovskite/charge-transport layer interfaces, such as is the case for the hole transport layer in our example, as a significant source of non-radiative charge recombination. These findings contribute to the understanding of transient ionic processes in perovskite-based devices.
Tax and Globalisation: Toward a New Social Contract
Authors: Dagan Tsilly 
Published: March 2024

Abstract
Taxation and representation are famously linked in the coercive co-authored project of political governance described through the social contract metaphor. Globalisation transforms this canonical account of the state. Many people can relocate and operate beyond state borders, consuming goods and services publicly offered by other jurisdictions. Expanding people’s opportunities to satisfy their preferences and pursue their goals supports their liberty. Yet, it also limits the ability of states to collect taxes so as to provide necessary public goods and secure justice, jeopardising the bond between taxation and equal membership in a political community. The challenge for taxation under globalisation is to revitalise the very basis of the social contract. Ideally, the new social contract should support the states’ just institutions and the collective self-determination of their members without rolling back the opportunities people have acquired through globalisation.
Correction to: Generalization of Hamiltonian mechanics to a three-dimensional phase space
Authors: Sato Naoki 
Published: March 2024

Abstract
In a recent paper [N. Sato, Prog. Theor. Exp. Phys. 2021, 6, 063A01 (2021)] we introduced a generalization of Hamiltonian mechanics to three-dimensional phase spaces in terms of closed 3-forms. This correction addresses an error in the proof of theorem 3, which concerns the existence of a coordinate change transforming a closed 3-form into a constant form. Indeed, invertibility of a 3-form is not sufficient to ensure the existence of a solution Xt to eq. (77) when n &gt; 3. The theorem can be corrected by restricting the class of 3-forms to those that are relevant to generalized Hamiltonian mechanics. Although the new theorem requires a stronger hypothesis, the formulation of dynamical systems with 2 invariants in terms of closed 3-forms, which is the key contribution of the paper, holds.
Create your own path: social cerebellum in sequence-based self-guided navigation
Authors: Li Meijia , Haihambo Naem , Bylemans Tom , Ma Qianying , Heleven Elien , Baeken Chris , Baetens Kris , Deroost Natacha , Van Overwalle Frank 
Published: March 2024

Abstract
Spatial trajectory planning and execution in a social context play a vital role in our daily lives. To study this process, participants completed a goal-directed task involving either observing a sequence of preferred goals and self-planning a trajectory (Self Sequencing) or observing and reproducing the entire trajectory taken by others (Other Sequencing). The results indicated that in the observation phase, witnessing entire trajectories created by others (Other Sequencing) recruited cerebellar mentalizing areas (Crus 2 and 1) and cortical mentalizing areas in the precuneus, ventral and dorsal medial prefrontal cortex and temporo-parietal junction more than merely observing several goals (Self Sequencing). In the production phase, generating a trajectory by oneself (Self Sequencing) activated Crus 1 more than merely reproducing the observed trajectories from others (Other Sequencing). Additionally, self-guided observation and planning (Self Sequencing) activated the cerebellar lobules IV and VIII more than Other Sequencing. Control conditions involving non-social objects and non-sequential conditions where the trajectory did not have to be (re)produced revealed no differences with the main Self and Other Sequencing conditions, suggesting limited social and sequential specificity. These findings provide insights into the neural mechanisms underlying trajectory observation and production by the self or others during social navigation.
The first insight into Mycobacterium tuberculosis complex isolates in the lower northern region in Thailand
Authors: Rudeeaneksin Janisara , Bunchoo Supranee , Phetsuksiri Benjawan , Srisungngam Sopa , Khummin Ratchaneeporn , Thapa Jeewan , Nakajima Chie , Suzuki Yasuhiko 
Published: March 2024

Abstract

Background
Tuberculosis (TB) remains an important infectious disease and different genotypes have been reported. This study aimed to investigate the genetic diversity and molecular epidemiology of TB in the lower northern region of Thailand, where genotyping data are limited.

Methods
A total of 159 Mycobacterium tuberculosis complex (MTBC) isolates from this region were genotyped by spoligotyping and the major spoligotypes were further subdivided by the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) method.

Results
Spoligotyping identified 34 types and classified them into 14 clusters. East African–Indian (EAI) groups were the most frequent (44.7%), followed by Beijing (36.5%), with a higher prevalence of drug resistance. By 15-loci MIRU-VNTR typing, the major groups of the Beijing and EAI2_NTB were further differentiated into 44 and 21 subtypes forming 9 and 5 subclusters with cluster rates of 0.26 and 0.44, respectively. The Hunter–Gaston Discriminatory Index among the Beijing and EAI2_NTB groups were 0.987 and 0.931, respectively, indicating high diversity.

Conclusions
This is the first look at the MTBC genotypes in the lower northern region of Thailand, which could aid in understanding the distribution and potential spread of MTBC and Mycobacterium bovis in the target region to support TB control in Thailand.
Predictors of 28-day mortality in melioidosis patients presenting to an emergency department: a retrospective cohort study from South India
Authors: Nisarg S , Tirlangi Praveen Kumar , Ravindra Prithvishree , Bhat Rachana , Sujir Sachin Nayak , Alli Sai Deepak , Chowdhury Soumi , Earny Venkat Abhiram , Gupta Nitin , Mukhopadhyay Chiranjay 
Published: March 2024

Abstract

Background
Septic melioidosis is associated with high mortality in resource-limited settings. The current study aims to find 28-d all-cause mortality predictors within 24 h of admission in melioidosis patients presenting to an emergency department.

Methods
This retrospective cohort study (2018–2022) included melioidosis patients divided into two groups based on their primary outcomes (28-d mortality). All the clinically relevant factors significant in univariate analysis were selected for binary logistic regression analysis. Those factors significant in logistic regression analysis were considered independent predictors of mortality.

Results
Of the 53 patients with melioidosis, the 28-d mortality of melioidosis patients admitted to the emergency department was 51% (n=27). Respiratory involvement, renal dysfunction, haemodynamic instability, elevated aspartate transaminase, elevated activated partial thromboplastin time, elevated CRP, elevated procalcitonin, decreased albumin, decreased absolute neutrophil count, decreased absolute lymphocyte count and use of piperacillin-tazobactam or azithromycin were significant predictors of mortality on univariate analysis. Vasopressor requirement (p=0.03) and low serum albumin level (0.041) at presentation were independent predictors of mortality.

Conclusion
Vasopressor requirement and low albumin levels at presentation in the emergency department are independent predictors of mortality. There is a need to create awareness among primary care physicians to enable early diagnosis and prompt initiation of treatment.
Tonic dendritic GABA release by substantia nigra dopaminergic neurons
Authors: Simmons DeNard V , Moreno-Ramos Oscar Andrés , Raj Divya D.A. , Kaganovsky Konstantin , Ding Jun , Awatramani Rajeshwar , Wilson Charles J. , Surmeier D. James 
Published: March 2024

AbstractRecent studies have demonstrated the importance of extrastriatal dopamine release in the emergence of the network dysfunction underlying motor deficits in Parkinson’s disease (PD). To better characterize the actions of dopamine on substantia nigra pars reticulata (SNr) GABAergic neurons, optogenetic and electrophysiological tools were used inex vivomouse brain slices to monitor synaptic transmission arising from globus pallidus externa (GPe) neurons. As predicted by previous work, activation of D2 dopamine receptors (D2Rs) suppressed GABA release evoked by stimulation of GPe axons. However, D2R activation also suppressed a tonic, GABAAreceptor-mediated inhibition of SNr spiking. D2R-mediated inhibition of tonic GABA release led to a roughly 30% increase in SNr spiking rate. Chemogenetic inhibition of GPe terminals or excitation of astrocytes did not affect tonic GABA release in the SNr. In contrast, chemogenetic inhibition of dopaminergic neurons or knocking down the expression of aldehyde dehydrogenase 1A1 (ALDH1A1) blunted tonic GABAergic signaling. Antagonizing D1 dopamine receptors on the terminals of striatonigral neurons also modestly increased SNr spiking. Lastly, in a progressive mouse model of PD targeting dopaminergic neurons, the tonic inhibition of SNr neurons by GABA release also was lost. Taken together, these observations suggest that dopamine and GABA are co-released by the dendrites of ALDH1A1-expressing dopaminergic neurons that course through the SNr. The co-release of these transmitters could serve to promote movement by making SNr neurons less responsive to phasic activity arising from the indirect pathway circuitry and by lowering basal spiking rates.
Sensory processing sensitivity is associated with neural synchrony and functional connectivity during threatening movies
Authors: Damatac Christienne G. , Homberg Judith R. , Galesloot Tessel E. , Geerligs Linda , Greven Corina U. 
Published: March 2024

ABSTRACTSensory processing sensitivity (SPS) is an evolutionarily conserved trait describing a person’s sensitivity to subtle stimuli, their depth of processing, emotional reactivity, and susceptibility to being overwhelmed. SPS is considered a fundamental and evolutionarily conserved trait, yet its neural mechanisms remain insufficiently understood. Therefore, we investigated whether SPS relates to processing movies differently in the central executive (CEN), default mode (DMN), and salience (SN) networks. We obtained positive and negative dimension Sensory Processing Sensitivity Questionnaire (short-form) scores and (neutral and threat aural framing) movie-fMRI data from a population-based sample (Healthy Brain Study, N=238, agemean=34years). We performeda prioriinter-subject representation similarity, activation, and inter-subject functional connectivity analyses to characterize SPS-dimension-related neural responses during movie-viewing. More similar negative dimension SPS score related to more neural synchrony in the CEN and SN during threat. Higher negative dimension SPS score related to reduced CEN-DMN functional connectivity during threat, an effect shared across between-network regions but most strongly driven by reduced connectivity between right dorsomedial prefrontal cortex and left lateral prefrontal cortex. Our findings suggest that highly sensitive individuals exhibit distinct CEN differences shaping environmental perception, process threat differently, and each SPSQ-SF dimension may involve unique neurological mechanisms.