bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of integrated hepatitis B virus DNA and cellular flanking sequences cloned from a hepatocellular carcinoma
Authors: Matsumoto Hisashi , Yoneyama Tetsuo , Mitamura Keiji , Osuga Toshiaki , Shimojo Hiroto , Miyamura Tatsuo 
Published: February 2007

AbstractBy digestion with HindIII restriction enzyme, a human hepatocellular carcinoma was shown to contain only 2 hepatitis B virus (HBV) DNA inserts. Both HindIII fragments (8 and 16 kb) were molecularly cloned and the structures of HBV DNA and adjacent host sequences were analyzed. One clone λYH 8) contained part of pre‐S(I) through the entire S gene and the other (λYH 16) had the middle section of the S to the end of X gene of HBV DNA. No gross rearrangements were observed in either HBV or cellular DNA sequences in λYH 16 clone. However, the 8 kb HindIII fragment was considered to be amplified together with flanking cellular sequences. Furthermore, the HBV DNA was integrated into cellular genome at the Alu repeated sequences in λYH 8.
Expression of the c‐fgr related transcripts in epstein‐barr virus‐associated malignancies
Authors: Klein Christine , Busson Pierre , Tursz Thomas , Young Lawrence S. , Raab‐Traub Nancy 
Published: February 2007

AbstractThe proto‐oncogene c‐fgr is expressed at high levels in cell lines derived from lymphomas which are infected with Epstein‐Barr virus (EBV) (Cheah et al., 1986). mRNA extracted directly from biopsies of EBV‐infected tissues was analyzed on Northern blots to determine if c‐fgr is expressed during lympho‐proliferations induced in vivo by EBV and in nasopharyngeal carcinoma (NPC), the epithelial malignancy associated with the virus. Elevated levels of c‐fgr expression were detected in all EBV‐positive lympho‐proliferations in vivo but not in cell lines established by EBV infection in vitro. This indicates that the induction of the c‐fgr proto‐oncogene is not an essential component of EBV‐induced transformation. Although no c‐fgr expression was detected in EBV‐positive or ‐negative epithelial cell lines, the 3.0‐kb c‐fgr mRNA was detected at low levels in mRNA obtained from NPC biopsy specimens. However, NPC tissue, after passage in nude mice (which eliminates infiltrating lymphoid and myeloid cells) did not contain the 3.0‐kb c‐fgr mRNA. The absence of expression of c‐fgr in the malignant epithelial cells infected with EBV contrasts with the elevated level of the proto‐oncogene in EBV‐infected lymphoma tissue and cell lines established from lymphomas. This suggests differences in the expression of cellular functions in EBV‐induced malignancies of these 2 distinct cell types.
Use of syngeneic monoclonal antibodies in the therapy of disseminated myeloid leukemic cells
Authors: Berends D. , Mulder A. H. , van Houwelingen G. , de Both N. J. 
Published: February 2007

AbstractA syngeneic monoclonal antibody (MAb) (IC5F5) was successfully used in the immunotherapy of Rauscher‐virus‐induced myeloid leukemic RMB‐1 cells. It is directed to a virus‐encoded, but aberrantly processed protein, which is expressed on the cell membrane. When applied in vivo, it binds only to RMB‐1 tumor cells. BALB/c mice were inoculated i.p. or i.v. with 107 RMB‐1 cells and died within 2–3 weeks due to increasing tumor load. Mice inoculated i.p. were completely cured by daily injections of ascites containing IC5F5. Disseminated tumor cells in liver and hemopoietic organs were observed after i.v. inoculation. Daily treatment with MAbs resulted in survival beyond 90 days. No antigenic modulation was observed when tumor tissue was analyzed 2–10 days after treatment. Treatment was successful even when therapy was postponed until day 5 following inoculation of tumor cells. When the number of ascites injections was reduced, survival was identical to that observed among repeatedly treated mice. Ten‐ and 100‐fold dilution of ascites fluid diminished the number of survivors, but still resulted in a median survival time of 38 and 20 days, respectively, as compared to 14 days for untreated mice.
Mast‐cell‐deficient W/Wv mice exhibit A decreased rate of tumor angiogenesis
Authors: Starkey Jean R. , Crowle Patricia K. , Taubenberger Simone 
Published: February 2007

AbstractThe role of host mast cells in tumor‐associated angiogenesis was investigated by comparing the angiogenic response of genetically mast‐cell‐deficient W/Wv mice and mast‐cell‐sufficient +/+ littermate mice to s.c. growing B16‐BL6 tumors. The angiogenic response was found to be slower and initially less intense in W/Wv mice than in +/+ mice. Fewer W/Wv mice than +/+ mice developed spontaneous lung metastases and W/Wv mice exhibited fewer lung metastases per mouse. Bone‐marrow repair of the mast‐cell deficiency restored the angiogenic response of W/Wv mice and also restored the incidence of hematogenous metastases to approach that of +/+ mice. Differences in lymphatic metastasis were not detected between W/Wv and +/+ mice. These results demonstrate a role for mast cells in vivo during tumor angiogenesis, and suggest a role also for host mast cells in hematogenous metastasis.
Correlation between urokinase‐type plasminogen activator production and the metastasizing ability of two murine mammary adenocarcinomas
Authors: Pereyra‐Alfonso Susana , Haedo Ana , de Kier Joffe Elisa Bal 
Published: February 2007

AbstractPlasminogen activator (PA) activity was studied in a transplantable murine mammary adenocarcinoma (M3), moderately metastatic to lungs, and in a highly metastatic variant tumor (MM3) to establish whether a correlation existed between this enzyme and the tumors' metastasizing abilities. The cell‐associated and secreted PA activities from primary cultures of both tumors, as well as from solid tumor homogenates, were quantitated by means of a fibrinolytic assay. Immunoneutralization and zymographic assays were done to identify the PA present in both tumors. In culture, the highly metastasizing MM3 cells produced (secreted plus cell‐associated activator) 3.3‐fold higher PA levels than the M3 cells. This difference was mainly attributed to the enhanced secreting ability of MM3, as these tumor cells secreted 102 times their cell‐associated PA activity within 24 hr, while M3 cells secreted only 11 times this activity during the same period. PA activity was also significantly higher in MM3 than in M3 tumor homogenates. Acid‐labile inhibitors and non‐specific proteases were not detected. In both tumors, PA was characterized as urokinase‐type, with a molecular weight of approximately 48 kDa. These results suggest that, in this model, PA plays a role in metastasis formation.
Alterations in c‐myc expression in relation to maturational status of human colon carcinoma cells
Authors: Mulder Kathleen M. , Brattain Michael G. 
Published: February 2007

AbstractPrevious work from this laboratory established a large bank of human colon carcinoma cell lines in culture and classified them with respect to growth regulatory phenotypes based on several biological and biochemical characteristics. In the present report, Northern analysis indicates that addition of the maturational agent N,N‐dimethylformamide (DMF) (1.0%) to proliferating HCT 116 and MOSER cells resulted in a repression of c‐myc proto‐oncogene expression; retinoic acid (1.0 μM) was less effective in this regard. Repression of c‐myc expression by DMF was observed in MOSER and HCT 116 cells, whether it was added to proliferating or late log phase cultures, and was associated with a corresponding reduction in cellular proliferation. The reduction in c‐myc expression by DMF did not require new protein synthesis and occurred within a few minutes after its addition, resulting in a 70% reduction within approximately 2 hr. Previous work from this laboratory indicated that transforming growth factor‐β (TGF‐β) elicited alterations in MOSER cells similar to those observed following DMF treatment. The present report demonstrates that proliferating, but not late log phase, MOSER cells responded to TGF‐β with a repression of c‐myc expression. Similarly, an inhibition of cellular proliferation was only observed when TGF‐β (10 ng/ml) was added to proliferating cells. Collectively, these findings indicate that repression of c‐myc expression is associated with diminished cellular proliferation and the induction of a more benign phenotype in human colon carcinoma cells. Furthermore, this report is the first demonstration of a c‐myc response to TGF‐β in an epithelial cell line.
Effect of retinoic acid on bovine papillomavirus (BPV) DNA‐induced transformation and number of BPV DNA copies
Authors: Tsang Siu Sing , Li Gang , Stich Hans F. 
Published: February 2007

AbstractThe effect of all‐trans‐retinoic acid (RA) was examined on (1) transformation induced in C127 cells by transfection with plasmid pdBPV‐1 (142‐6), which contains DNA of bovine papillomavirus (BPV), (2) the capacity of transformed BPV DNA‐containing clones to form colonies with transformed properties (e.g., piling up into multilayered colonies), and (3) the number of BPV DNA copies in transformed cells. At nontoxic doses ranging from 10−7 to 10−5M, RA reduced the frequency of transformed foci in a dose‐dependent manner. The extent of inhibition depended on the length of RA treatment and on the time that elapsed between DNA transfection and RA treatment. RA exerted only a slight inhibitory effect during the first days after transfection. Complete inhibition was observed when the cells were continuously exposed after transfection to RA at doses ranging from 0.5 to 1 × 10−5M. The inhibitory effect of RA on transformation was reversible. Transformed foci started to form after withdrawal of RA treatment. In the presence of RA (5 × 10−6M), cells from transformed colonies were no longer able to form foci displaying transformed properties. The number of BPV DNA copies gradually decreased when the cells were grown over several generations in the presence of RA (5 × 10−6M). After approximately 30 cell generations, the cell cultures appeared to have less than one copy of BPV DNA.
New cell line from hairy‐cell leukemia: Confirmation of leukemic cell origin by karyotype and IG gene analysis
Authors: Tokumine Yukihiro , Ueda Etsuko , Ogawa Hiroyasu , Sugiyama Haruo , Taniwaki Masafumi , Abe Tatsuo , Kanayama Yoshio , Hashimoto Tomoko , Inoue Ryoichi , Machii Takashi , Kitani Teruo 
Published: February 2007

AbstractA hairy‐cell leukemia (HCL) line, BNBH‐1, was established from the peripheral blood of a 40‐year‐old male patient with HCL in a relatively stable clinical phase after splenectomy. The cells have since been growing continuously for more than 2 years. Their cell surface immunoglobulin (slg) was identical with that found on the surface of freshly isolated leukemic cells, consisting of IgG‐K. The BNBH‐1 cells were more mature than the original hairy cells in their degree of B‐cell differentiation, as reflected by a decrease in slg expression together with the appearance of some cytoplasmic lg (clg)+ cells, loss of EAγ‐rosette formation and reactivity with monoclonal antibody (MAb) FMC7, and an increase in the proportion of MAb PCA‐1+ cells. The BNBH‐1 cells possessed the antigen recognized by Leu‐MS, a highly specific MAb for HCL. Epstein‐Barr virus nuclear antigen (EBNA) was present. Both the freshly isolated leukemic cells and the cell line had the 14q + involving q32 chromosomal abnormality, and their lg gene rearrangements were also identical. Following exposure to 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), both the freshly isolated leukemic cells and the BNBH‐1 cells adhered to culture dishes and extended long, thin processes, a phenomenon characteristic of HCL. These results indicate that the BNBH‐1 line was derived from the leukemic hairy cells.
Characterization of a human ovarian carcinoma cell line, OTN 14, derived from a mucinous cystadenocarcinoma
Authors: van Niekerk C. C. , Poels L. G. , Jap P. H. K. , Smeets D. F. C. M. , Thomas C. M. G. , Ramaekers F. C. S. , Vooijs G. P. 
Published: February 2007

AbstractA human ovarian carcinoma cell line, OTN 14, has been established from malignant ascitic fluid of a patient with a well‐differentiated mucinous cystadenocarcinoma of the left ovary. The cell line has been maintained in vitro for 6 months through 23 passages, growing in monolayers as well as in 3‐dimensional clusters, with a population doubling time of 281/2 hr. The number of chromosomes per cell varied from 67 to 88, with a modal number of 86. Two characteristic marker chromosomes were recognized, consisting of partially deleted chromosome I. With a DNA index of 1.934 the tumour cell line was near tetraploid. The epithelial character of the OTN 14 cells was confirmed by a positive immunofluorescence reaction with monoclonal antibodies (MAbs) against different keratins, and when (immuno)electron microscopy was used, keratin filaments and small junctional complexes were observed. Vimentin was also expressed in these cells, while desmin was not detected. Cultured tumour cells reacted (weakly) positive with MAb OV‐TL 3 as a marker for ovarian carcinomas, while reactivity with the anti‐ovarian carcinoma MAb OC 125 was limited to a few cells, not permitting the detection of shed CA 125 antigen in the culture supernatant. Cells stained heterogeneously positive for CEA marker BW 431/31, the presence of which was confirmed by detection of CEA shed into the culture medium. The cell line released estradiol at a concentration of 130,000 pmol/L in the culture medium, while no progesterone or dehydroepiandrosterone sulphate were found. Electron microscopical evidence for steroid production was suggested in some cells showing “dense‐core” vesicles near the Golgi areas. The OTN 14 tumour cells formed poorly differentiated tumour nodules in nude mice, and metastatic cells were also found in blood capillaries. Cell types with mucinous as well as endocrine characteristics were found.
Syngeneic monoclonal antibodies directed against rauscher virusinduced myeloid leukemic cells: Isolation and characterization
Authors: Berends D. , Rhijnsburger E. H. , van Gaalen J. L. M. , van Houwelingen G. , Zondervan P. E. , de Both N. J. 
Published: February 2007

AbstractHybridomas producing syngeneic monoclonal antibodies (MAbs) were prepared by fusion of spleen cells of BALB/c mice, which were immunized with sublethal doses of RMB‐1 cells. This cell line originates from a Rauscher virus (R‐MuLV)‐induced myeloid leukemia and forms tumors when re‐inoculated into mice. MAbs were characterized as regards their reactivity against virally and non‐virally induced cell lines. Two selected MAbs, ICSF5 and 4D2B4, were analyzed further. Their binding to subcellular structures was determined, and so were the properties of the antigens to which they are directed. MAb IC5F5 is of the IgG2A and 4D2B4 of the IgG2b subclass. Both bind to R‐MuLV‐infected or ‐transformed cell lines and are not mutually competitive. The antibodies do not react with other murine and human myeloid leukemic cells. As shown by immuno‐electron microscopy, these MAbs have affinity to the cell membrane of non‐virus producing RMB‐1 cells. When lysates of purified virus were analyzed, the MAbs were found to be directed to the gag precursor protein Pr65, and one of them (IC5F5) also to be directed to the core protein p12. In RMB‐1 cells, binding occurs to a 50‐kDa glycoprotein and 2 proteins of 26 and 29 kDa. Since RMB‐1 cells do not produce virus, but express aberrant viral proteins, these MAbs are tumor‐specific and useful for immunotherapy.
Anti‐tumor effects of tumor necrosis factor alone or combined with radiotherapy
Authors: Sersa Gregor , Willingham Veronica , Milas Luka 
Published: February 2007

AbstractRecombinant human tumor necrosis factor (rHuTNF) was investigated for its ability to increase the response of murine tumors to ionizing radiation. Both multiple i.v. administrations of rHuTNF and local tumor irradiation caused a significant delay in tumor growth. The effect of treatment with both agents combined was greater than the additive effect of the individual treatments. Furthermore, rHuTNF significantly increased tumor radiocurability, as assessed by the TCD50 assay. rHuTNF was not cytotoxic to tumor cells, nor did it affect their radiosensitivity. The in vivo anti‐tumor effect of rHuTNF and its augmentation of tumor radioresponse were mediated through indirect mechanisms, either immunological or non‐immunological. rHuTNF was also effective in reducing the damaging effect of ionizing radiation on bone‐marrow progenitor cells, which could increase the therapeutic advantage of the rHuTNF‐radiotherapy combination. These experiments suggest that rHuTNF is potentially beneficial in combination with radiotherapy.
Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer
Authors: Guelstein V. I. , Tchypysheva T. A. , Ermilova V. D. , Litvinova L. V. , Troyanovsky S. M. , Bannikov G. A. 
Published: February 2007

AbstractThe distribution of keratins 8 and 17 and of vimentin in 28 normal human mammary tissue samples, 16 benign tumors, 26 fibrocytic diseases and 52 malignant breast tumors have been studied using monoclonal antibodies H1, E3 and NT30, respectively. Three cell populations in normal mammary epithelium have been identified: luminal epithelium containing keratin 8, myoepithelium of the lobular structures positive for vimentin, and myoeplthelium of extralobular ducts positive for keratin 17. In different kinds of benign tumor and dysplastic proliferation a mosaic of cells with all normal phenotypes has been observed. The majority of cells co‐expressed keratins 8 and 17 or vimentin. In the overwhelming majority of carcinomas, cells did not contain myoepithelial markers (keratin 17 and vimentin) but expressed only keratin 8 specific to normal luminal epithelium.
Case‐control study of proximal and distal colon cancer and diet in Wisconsin
Authors: Young Theresa B. , Wolf Dennis A. 
Published: February 2007

AbstractThe relationship between diet and subsite‐specific colon cancer was investigated using dietary histories obtained from a statewide, population‐based sample of 152 proximal colon cancer patients, 201 distal colon cancer patients and 618 general population controls. The results do not support hypotheses that (1) dietary fat and cholesterol are more strongly related to proximal colon cancer and (2) vegetables and other dietary sources of fiber are more strongly associated with distal colon cancer. Vegetable consumption over lifetime was consistently protective for both proximal and distal colon cancer. Odds ratios and 95% confidence intervals for the most significant dietary factors (based on high vs. low consumption) for proximal colon cancer were: salad, 0.29 (0.17, 0.48); miscellaneous vegtables, 0.58 (0.35, 0.97); cruciferous vegetables, 0.59 (0.35, 0.97); processed lunchmeat, 2.04 (1.31, 3.17); panfried foods, 1.79 (1.15, 2.80); eggs, 1.75 (1.02, 2.99) and for distal colon cancer they were: salad, 0.43 (0.28, 0.67); cruciferous vegetables, 0.44 (0.28, 0.71); cheese, 0.62 (0.40, 0.96); processed lunchmeat, 1.79 (1.17, 2.73); pan‐fried foods, 1.55 (1.03, 1.27). The results support recommendations that the “prudent diet” (low‐fat, high‐vegetable) may reduce colon cancer risk.
Prognostic value of ebv markers in the clinical management of nasopharyngeal carcinoma (NPC): A multicenter follow‐up study
Authors: De‐Vathaire F. , Sancho‐Garner H. , De‐Thé H. , Pieddeloup C. , Schwaab G. , Ho J. H. C. , Ellouz R. , Micheau C. , Cammoun M. , Cachin Y. , De‐Thé G. 
Published: February 2007

AbstractBetween December 1979 and April 1982, 373 patients with untreated, undifferentiated carcinoma of the nasopharynx (NPC), 99 in Hong Kong, 120 in Tunis and 154 in Villejuif, entered a longitudinal study aimed at determining the clinical prognostic value of EBV serology after radiotherapy. A minimum of 3 years' follow‐up was achieved for 319 patients (83 in Tunis, 95 in Hong Kong and 141 in Villejuif) who had regular clinical and serological testing at intervals of 6–8 months. No significant difference in initial serology (i. e., before any treatment) or variations of antibody titers at time of first followup was observed between patients who achieved complete remission after radiotherapy and those who did not. This included IgG and IgA antibodies to VCA, EA or EBNA. However, when patients with confirmed clinical remission I year after completion of radiotherapy were studied, the value of IgG/EA and mainly of IgA/EA increasing titers became highly significant for prediction of relapse, regardless of the initial titers. This demonstrated the clinical usefulness of EBV serology for NPC patients who have confirmed clinical remission after radiotherapy.
Monoclonal antibodies produced by immunization with neoglycoproteins containing galα1‐4galβ1‐4glcβ‐O and galα1‐4galβ1‐4glcnacβ‐O residues: Useful immunochemical and cytochemical reagents for blood group p antigens and a differentiation marker in burkitt lymphoma and other B‐cell malignancies
Authors: Brodin N. Thomas , Dahmén Jan , Nilsson Bertil , Messeter Lisbeth , Mårtensson Stig , Heldrup Jesper , Sjögren Hans Olov , Lundblad Arne 
Published: February 2007

AbstractSeveral monoclonal antibodies (MAbs) directed to blood group P1 (Galα1‐4Galβ1‐4GlcNAcβ‐O) and Pk (Galα1‐4Galβ1‐4Glcβ‐O) determinants were produced with high efficiency by using synthetic neoglycoproteins as immunogens. The specificity of IgM and IgGI MAbs was characterized by binding to defined oligosaccharides and glycoconjugates. Antibodies that bound equally well to P1 and Pk determinants and to Galαl‐4Galβ1‐O‐derivatives were obtained, together with others that showed selective recognition of the entire trisaccharide chain. Selected antibodies were shown to be useful as reagents for detection of the blood group P antigens in glycolipid extracts of erythrocytes and on the surface of erythrocytes of different P phenotypes, demonstrated both by radioimmune assays and hemagglutination. Six IgM MAbs directed to the Pk determinant bound selectively to Burkitt lymphoma cells and 2 of these antibodies (424/3D9 and 424/6A2) could be used as auxiliary reagents in immunofluorescence for diagnosis and classification of B‐cell lymphomas and leukemias using flow cytometric analysis. Eight normal individuals and 37 patients with lymphoma or leukemia were studied. Tumor cells of 2/2 patients with “Burkitt‐like” lymphoma, 1 patient with centroblastic lymphoma and 2 patients with acute leukemia were strongly stained for the Pk antigen. The staining patterns for differentiation markers classified the tumor cells to a developmental stage closely related to the Burkitt cell type.