bluuebunny/crossref_metadata_2025_split huggingface dataset:
CYP2E1 deficit mediates cholic acid-induced malignant growth in hepatocellular carcinoma cells
Authors: Hao Zhiwei , Liu Xuemin , He Huanhuan , Wei Zhixuan , Shu Xiji , Wang Jianzhi , Sun Binlian , Zhou Hongyan , Wang Jiucheng , Niu Ying , Hu Zhiyong , Hu Shaobo , Liu Yuchen , Fu Zhengqi 
Published: March 2024

Abstract
Background
An increased serum cholic acid (CA) is concurrently appeared with a decreased CYP2E1 expression in hepatocellular carcinoma (HCC). However, whether and how CA may be involved in promoting hepatocarcinogenesis has not been elucidated. This study was aimed to investigate the role of CYP2E1 on CA-induced HCC cell growth and its underlying mechanism.
Methods
Our proteomic analysis of liver tumor tissues from DEN-induced male SD rats treated with CA administration revealed that CA downregulated CYP2E1 level. The proliferative ability of CA-treated HCC cells was examined by colony formation assays. Autophagic flux was detected by immunofluorescence and confocal microscopy. The protein levels of CYP2E1, mTOR, AKT, p62 and LC3Ⅱ were detected by Immunoblotting. The effect of CYP2E1 on CA-induced hepatocellular carcinogenesis was explored in vivo by establishing a xenograft tumor model in nude mice. We also investigated the clinical value of CYP2E1 in HCC patients.
Results
CA increased the clonogenicity of HCC cells and promoted the growth of xenograft tumors with a simultaneous reduction of CYP2E1 expression. Further studies revealed that both in vitro and in vivo, upregulating CYP2E1 could effectively inhibit cell growth of HCC with a blockage of autophagic flux, downregulation of AKT phosphorylation and upregulation of mTOR phosphorylation. CYP2E1 was involved in CA-activated autophagy through the AKT/mTOR signaling, which promoted CA-stimulated HCC cell growth. Finally, a decreased CYP2E1 expression was observed in the tumor tissues of HCC patients and the CYP2E1 level in tumor was negatively correlated with the serum level of total bile acids (TBA) and gamma-glutamyltransferase (GGT).
Conclusions
CYP2E1 deficit contributes to CA-induced HCC development with the mechanisms involving regulation of autophagy, thus CYP2E1 may serve as a potential target for HCC drug development.
Investigation Potential Antidiabetic Activity of Angelica gigas Nakai Leaf Extract
Authors: Lee Jaehak , Taufik Iqbal Maulana , Lim Seungtae , Kim Gur-Yoo , Jhoo Jin-Woo 
Published: March 2024

Abstract

This study was conducted to investigate Angelica gigas Nakai leaf extract (ALE) as an antidiabetic. The inhibitory activity of ALE on α-glucosidase inhibition and rat intestinal disaccharidase were measured. Also, its effect on glucose uptake in 3T3-L1 cells was analyzed. Supplementation of ALE up to 150µg/mL showed cell viability higher than 90% in 3T3-L1 cells. The 60% and 80% of ethanol ALE revealed the highest α-glucosidase inhibitory activity. Further, the 60% ethanol ALE extracts were freeze-dried (FD60) and spray-dried (SD60) to evaluate their antidiabetic effects. The α-glucosidase inhibitory assay revealed that the IC50 of SD60 and FD60 were 4.08 mg/mL and 2.93 mg/mL, respectively. A Lineweaver-Burk plot showed that ALE demonstrates mixed-type inhibition. FD60 and SD60 showed no significant difference in inhibiting maltase, sucrase, and glucoamylase of rat intestinal disaccharidase. FD60 and SD60 significantly (p < 0.05) increased insulin-stimulated glucose uptake in 3T3-L1 cells compared to D-pinitol. Moreover, ALE could increase the expression of pAkt Thr308 in Vero 76 cells via western blot analysis. These findings suggest that ALE has potential antidiabetic properties.
Efficacy and safety of PD‑1 monoclonal antibody combined with interferon‑alpha 1b and anlotinib hydrochloride as the second-line therapy in patients with unresectable advanced melanoma: a retrospective study
Authors: Zhao Bolun , Tang Jingyi , Zhang Mengyu , Zou Daopei , Liu Fang , Feng Ting , Li Chunying , Shi Qiong , Gao Tianwen , Zhu Guannan 
Published: March 2024

Abstract
Introduction
Immune-checkpoint inhibitors are now used more commonly in combination than monotherapy as the first-line choice in patients with unresectable advanced melanoma. Nevertheless, for cases that progressed after the initial combination therapy, the subsequent regimen option can be very difficult. Herein, we reported the efficacy and safety of a triple combination regimen in Chinese unresectable advanced melanoma patients who had poor responses to the first-line immune therapy.
Materials and Methods
We reviewed the clinical profiles of patients diagnosed with stage IIIC-IV melanoma between June 1, 2020, and September 30, 2023. The patients who failed the prior immune therapies and received anti-PD-1 mono antibody plus interferon(IFN)‑alpha 1b and anlotinib hydrochloride as the second-line therapy were enrolled in the retrospective analysis. Additionally, we examined the exhaustion of T-cells using mIHC staining in available tumor samples.
Results
56 patients were included in this study. The median follow-up period was 13.6 months. The objective response rate evaluated by the investigators was 8.9%(1CR, 4PR). The disease control rate was 46.4%. The median overall survival was 17.5 months, and the median progression-free survival was 2.8 months. The adverse events rate of any grade was 100%. Grade 3 or 4 irAEs were observed in 28.6% of cases. Multiplex immunohistochemical staining revealed an increased trend of TIM3 expression on tumor-infiltrating T cells in patients without objective response.
Conclusion
PD‑1 monoclonal antibody plus interferon‑alpha 1b plus anlotinib showed acceptable tolerability and anticancer benefits in Chinese metastatic melanoma patients as a second-line therapy.
An improved density peaks clustering algorithm use similarity assignment strategy with K-nearest neighbors
Authors: Hu Wei , Feng Ji , Yang Degang 
Published: March 2024

Abstract
Some particular shaped datasets, such manifold datasets, have restrictions on Density Peak Clustering (DPC) performance. The main reason of variations in sample densities between clusters of data with uneven densities is not taken into consideration by the DPC algorithm, which could result in the wrong clustering center selection. Additionally, the use of single assignment method is leads to the effect of assignment errors. To address these problems, this paper creates a new, improved density peaks clustering method use the similarity assignment strategy with K nearest Neighbors (IDPC-SKNN). Firstly, a new method for defining local density is proposed. Local density is comprehensively consider in the proportion of the average density inside the region, which realize the precise location of low-density clusters. Then, using the samples' K-nearest neighbors information, a new similarity allocation method is proposed. Allocation strategy successfully address assignment cascading mistakes and improves algorithms robustness. Finally, based on four evaluation indicators, our algorithm outperforms all the comparative clustering algorithm, according to experiments conducted on synthetic and real world datasets.
Exclusive Breastfeeding Practice and Associated Factors among Mothers Having Infants (Aged 0-6 Months) in the Pastoral Community of Sawena District, Bale Zone Oromia, Ethiopia, 2023
Authors: Hussein Dursa , Girma Derara , Oyato Befekadu Tesfaye , Birhanu Belete , Teressa Beka , Bacha Sintayew , Geleta Rabbira Edessa , Ibrahim Seifu Mohammed , Lakew Meron Seyoum 
Published: March 2024

Abstract
Background: Breastfeeding is the easiest, healthiest, and most cost-effective feeding method forinfants. Despite the promotion of exclusive breastfeeding, its importance is poorly understood in pastoral communities. Little information is available on the practices and factors underlyingexclusive breastfeeding, especially in pastoral communities in the Sawena district, Bale zone, Oromia region, and Ethiopia.
Objective: This study aimed to assess the prevalence of and factors associated with exclusive breastfeeding among mothers with infant (aged 0-6 months) in pastoral communities in the Sawena district, Bale Zone, Oromia Region, SoutheastEthiopia, 2023.
Method: A community-based cross-sectional study design was employed from March 01 to April 2023. A total of 631 women were selected using simple random sampling. The data were collected through face-to-face interviews. The collected data were entered into Epi Data software version 4.6 and subsequently exported into SPSS version 26 for analysis. Variables with a P value < 0.25 in the bivariate analysis were included in the multivariable logistic regression model. The degree of association was expressed using adjusted odds ratios (AOR) with a 95% confidence interval (CI) of < 0.05.
Results: Out of 631 eligible mothers, 625 participated in this study, for a response rate of 99.0%. The prevalence of exclusive breastfeeding (EBF) practices among mothers in the study area was 53.8% (95% CI; 49.8%–57.4%). Household food security status [AOR=1.6, 95% CI (1.1-2.2)], ANC follow-up during pregnancy, [AOR = 5.3, 95% CI (2.4-11.9)], counselling about EBF during ANC visits[AOR=5; 95% CI (2.1-11.7)], number of children [AOR =1.6, 95% CI (1.1-2.3)] and attitudes toward EBF [AOR= 1.8; 95% CI (1.4-2.6)] were significantly associated with EBF.
The majority of interviewees explained that there were various reasons why exclusive breastfeeding was not practiced, including beliefs that breast milk was insufficient, giving babies water would reduce colic, and fears of food refusal.
Conclusion: The prevalence of exclusive breastfeeding practices in the study area was lower than the country-recommended level. Household food security status, ANC follow-up during pregnancy, counselling about breastfeeding during ANC, number of children and attitude toward EBF practice were significantly associated with EBF. BF counselling during ANC services can be provided to mothers to improve their BF knowledge and subsequently improve their EBF practices.
Piloting the co-culture of Gracilaria verrucosa seaweed and Penaeus monodon in extensive pond systems in Java, Indonesia
Authors: Nauta Reindert Wieger , Lansbergen Romy Alana , Ariyati Restiana Wisnoe , Widowati Lestrari Lakhsmi , Rejeki Sri , Debrot Adolphi Oscar 
Published: March 2024

Abstract

With an increasing need for food for the growing global human population, more food will have to be produced sustainably with a lower impact on the environment while still being economically profitable. We studied the effect of combining seaweed cultivation with extensive shrimp farming on production, gross profit and nutrient concentrations. Pond experiments were conducted using different combinations of the agaroid seaweed Gracilaria verrucosa, and the Giant tiger prawn, Penaeus monodon, without supplemental feeding. Combined cultivation showed 18% higher seaweed production during the first cycle and 27% higher production during the second. Shrimp production was 53.8% higher in combined cultivation than in single-species cultivation. Seaweed quality in terms of agar content and gel strength did not differ between treatments. Gross profit of combined cultivation was, respectively, 156% and 318% higher compared to single-species seaweed and shrimp cultivation. As no feed was used, environmental impact was reduced using this method. Seaweed culture decreases nutrient loading in the water and pond sediment when cultured either solely or in combination with shrimp and lowered bacterial counts. Co-cultivation therefore improves the income whilst reducing the environmental impact.
An Isolable Phosphinidene Oxide
Authors: Goicoechea Jose , Hu Chenyang , Rees Nicholas , Pink Maren 
Published: March 2024

Abstract

Phosphorus monoxide (PO•) was the first compound with a P–O bond to be spectroscopically detected in interstellar space. On Earth, PO•, and related functionalized molecules such as R–P = O (R = OH, CH3, OCH3, Ph), have been studied in inert gas matrices at ultralow temperatures (3–15 K). These species are believed to be key intermediates in the degradation/combustion of organic phosphorus compounds (a class of chemicals that includes chemical warfare agents and flame retardants). However, despite numerous attempts, an isolable example of R–P = O has eluded synthetic chemists to date. Here we describe the use of an extremely bulky amine ligand to isolate the first two-coordinate phosphorus(III) oxide. Reactivity studies on this and other related compounds shows that phosphorus center can be readily oxidized, and that in doing so, the P–O bond remains intact, an observation that is of interest to the proposed reactivity of phosphorus monoxide (PO•).
Non-acidotic hypercapnia limits atrophy and loss of specific force in rat diaphragm after 5 days of controlled mechanical ventilation in parallel with increased local inflammation
Authors: Cacciani Nicola , Addinsall Alex B. , Larsson Lars 
Published: March 2024

Abstract
Background
Controlled Mechanical Ventilation (CMV) is associated with Ventilator Induced Lung Injury (VILI) and Ventilator Induced Diaphragm Dysfunction (VIDD). VIDD delays weaning from the respirator and increases the risk of further complications and health care costs, which are disproportionately increased with increasing duration of mechanical ventilation. Hypercapnia is frequently observed and tolerated as “permissive hypercapnia” during lung protective MV strategies. The systemic effects of hypercapnia are well known and considered potentially protective for diaphragm muscle in acute and short-time experimental MV studies. However, hypercapnia is commonly associated with acidosis, affecting immunity and inflammation pathways.
Methods
This study aims to determine the potential of hypercapnia in the absence of acidosis on diaphragm muscle structure and function in a well-established clinically relevant experimental ICU model, not limited by early mortality. The effects of hypercapnia at physiological pH on diaphragm single fibers cross sectional area (CSA) and specific force (maximum force normalized to CSA) were investigated.
Results
Non-Acidotic Hypercapnia (NAH) reduced body mass loss, diaphragm muscle fiber atrophy and loss of specific force, in parallel with an increased gene expression of proinflammatory cytokines (TNF-α and IL-1β) and of the MuRF-1 atrogene. In the diaphragm, TNF-α gene expression was significantly increased in NAH rats compared with 5 days normocapnic and controls, while IL-1β showed an increasing trend. In the lung lysates, IL-1β gene expression was significantly increased in 5 days normocapnic rats compared with the controls, while gene expression of TNF-α was increased in the NAH rats compared with controls. In NAH rats the increase was not significant. The gene expression of mitochondrial factors TFAM (regulator of mitochondrial gene expression), MFN2 (involved in mitochondrial fusion, quality control and cell metabolism), PARKIN (involved in mitochondrial quality control and mitophagy), ULK-1 (activator of mitophagy) was analyzed. NAH reversed, significantly the decreased gene expression of ULK 1 observed in the 5 days normocapnic rats.
Conclusions
These results suggest that non-acidotic hypercapnia limits the development of VIDD, irrespective of amplified local muscle inflammation. Therefore, we suggest its clinical role may be complementary to the known anti-inflammatory effects of hypercapnic acidosis (HCA), which has preventive VIDD effects as well.
Individual differences in processing multisensory information predict presence in different virtual reality environments
Authors: Glenn Callum P , Coxon Matthew 
Published: March 2024

Abstract

One of the most notable aspects of using a head mounted display (HMD) is the feeling of being ‘within’ the digitally created virtual world. Technological advances across the fields of computer science and engineering have helped to increase this sense of presence. However, there remains wide variations between individuals, which are only just beginning to be captured at a theoretical level. One potential explanation for these individual differences may be how individuals process multisensory information. In this paper we detail two studies which explored whether performance on two different multisensory tasks (the pip and pop and a redundant signals task), predict some of these differences in self-reported presence. In study 1 (N=32), clear correlations between the multisensory task (pip and pop) and presence scores were found using an underwater simulation. This provided the first indication that individuals that were positively influenced by illusory audiovisual conjunctions were also those that had the strongest sense of presence. Study 2 (N=68) expanded upon these results, finding again that multisensory integration (within a redundant signals task) was related to self-reports of presence using a different VR experience. In addition, it was found that this relationship could be moderated by either providing a unisensory or multisensory VR experience. Together these results suggest that individual differences in the efficiency of multisensory integration may provide an additional level of explanation not currently accounted for within theoretical models of presence formation.
Multi-criteria decision-making approach for evaluating wastewater treatment plant performance using the Analytical Hierarchy Process : a case study of the Sebdou textile industry treatment plant (NW- Algeria)
Authors: Abdelmoumene Hanane , Lallam Faiza , Boumediene Maamar 
Published: March 2024

Abstract
The inefficiency of wastewater treatment plants (WWTP) for the removal of pollution has devastating effects on human health, negative impacts on the environment, and unfavorable consequences for economic activity. The activated sludge process is extensively used for wastewater treatment across the globe, especially in Algeria. While the purification performance and reliability of this process have been established, various types of biological malfunctions can occur. Our objective is to provide practical guidance in identifying these malfunction types. The evaluation of a wastewater treatment plant's (WWTP) malfunction degree is a crucial step in assessing its purification performance. This study aims to evaluate the operational performance of a textile industry WWTP situated in our region. The study focused on the wastewater treatment plant (WWTP) at the DENITEXT textile complex in Sebdou, Western of Algeria. The performance of the plant was analyzed with the help of the Analytical Hierarchy Process (AHP), a method that considers all criteria impacting that have an impact on the malfunctioning of the WWTP's malfunction and their relative importance. To evaluate the dysfunction score and the operational status of the treatment plant, a hierarchical multicriteria analysis (HMA) was used.The value of the SDG (Score Dysfunction Global), evaluates the performance of the WWTP in treating effluent, obtained 1.50, which falls within the range of [0, 2], indicates that the treatment plant is in a dysfunctional state but poses no present or future risks. The purpose of this work is to assist plant managers in effectively managing the wastewater treatment process of the plant. Currently, there are no known or potential risks.
A missense c.80G＞A mutation in the CAV3 gene in a Han Chinese family with rippling muscle disease
Authors: Lu Xiaoqing , Hu Fan , Li Lingjuan , Zhang Honglian , Fu Saifang , Zhang Hong , Li Jie , Nie Hongbing , Huang Gang , Shen Yaoyao 
Published: March 2024

Abstract

Background
Rippling muscle disease (RMD), one of the special clinical phenotypes of caveolinopathies, is characterized by muscle hyperexcitability causing by mutations in the CAV3 gene. Few data exist on muscle MRI in patients with RMD, frequently reported as normal.
Methods
Here, we describe a Han Chinese family with RMD. Whole exome sequencing (WES), muscle MRI, and tissue biopsy were performed in this study.
Results
The proband and his mother presented with RMD, which was initially noticed during their childhood or adolescence, accompanied with increased level of serum creatine kinase (CK). Biopsy specimen illustrated slight variation in fiber size and increased numbers of internal nuclei in the muscle fibers, without obvious atrophy. Muscle MRI of the thighs showed short-tau inversion recovery (STIR) hyperintensity without fatty replacement. WES identified a heterozygous missense mutation of c.80G>A (p.R27Q) in the CAV3 gene.
Conclusions
Our findings deepen our awareness of the muscle MRI and pathological features of hereditary RMD. Regarding muscle MRI, this study first reports STIR hyperintensity in the thighs, which may be linked with muscle oedema.
Kelvin-Helmholtz instability in 3He superfluids in zero temperature limit
Authors: Eltsov V.B. , Hosio J.J. , Krusius M. 
Published: March 2024

Abstract

In rotating 3He superfluids the Kelvin-Helmholtz (KH) instability of the AB interface has been found to follow the theoretical model above 0.4 Tc. A deviation from this dependence has been assumed possible at the lowest temperatures. Our NMR and thermal bolometer measurements down to 0.2 Tc show that the critical KH rotation velocity follows the extrapolation from higher temperatures. We interpret this to mean that the KH instability is a bulk phenomenon and is not compromised by interactions with the wall of the rotating container, although weak pinning of the interface to the wall is observed during slow sweeping of the magnetic field. The KH measurement provides the only so far existing determination of the interfacial AB surface tension as a function of pressure and temperature down to 0.2 Tc.
Endogeneity, Heterogeneity and Determinants of Inefficiencies in Crop-producing Farmers: Empirical Evidence from Central Highlands of Ethiopia
Authors: BERISSO Oumer 
Published: March 2024

Abstract
This study estimated transient and persistent inefficiencies distinguished from farm-heterogeneity and endogeneity forEthiopian grain crop-producing farmers for the period 2004 – 2015. The study used Mundlak-adjusted random effect – four error component stochastic frontier (4eCSF) model byextending earlier version of the 4eCSF model to distinguished endogeneity and farm-heterogeneity from time-invariant inefficiency and to explain inefficiencies. The Mundlak-adjusted model is then estimated using multi-step estimation. The estimated average values of persistent, transient and overall efficiencies were 79%, 73% and 58%, respectively indicating there was room for improvement using the present resource and technology. Result from inefficiency effect model reveals sex, family size, number of plots, owing oxen, land quality and rainfall influenced transient inefficiency negatively while age, education, and temperaturevariation influenced positively. Persistent inefficiency was influenced negatively by owing oxen, irrigation, land quality, farm size, population pressure, rainfall and distance to market whereas age and education affected positively. The findings exhibit a low overall technical efficiency of Ethiopian grain crop-farmers is mainly due to the transient part. On the other hand, though the level of persistent inefficiency is lower than that of transient inefficiency, it is still substantial. Hence, these findings are important to initiate government policy options to reduce inefficiency focusing on factors affecting the long-run and short-run inefficiencies distinctly. The current low level of efficiency can be addressed by increasing farmers’ access to education, modern agricultural machineries and input market accesses, improving technologies that promote land conservation (quality) and reduce weather adverse effects and support for female farmers.
JEL Classification: C23; C33; D24; O13; Q12; Q18.