bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional characterization and transcriptional analysis of degQ of Xanthomonas campestris pathovar campestris
Authors: Lo Hsueh‐Hsia , Chang Hsiao‐Ching , Wu Yi‐Jyun , Liao Chao‐Tsai , Hsiao Yi‐Min 
Published: March 2024

AbstractHigh‐temperature‐requirement protein A (HtrA) family proteins play important roles in controlling protein quality and are recognized as virulence factors in numerous animal and human bacterial pathogens. The role of HtrA family proteins in plant pathogens remains largely unexplored. Here, we investigated the HtrA family protein, DegQ, in the crucifer black rot pathogen Xanthomonas campestris pathovar campestris (Xcc). DegQ is essential for bacterial attachment and full virulence of Xcc. Moreover, the degQ mutant strain showed increased sensitivity to heat treatment and sodium dodecyl sulfate. Expressing the intact degQ gene in trans in the degQ mutant could reverse the observed phenotypic changes. In addition, we demonstrated that the DegQ protein exhibited chaperone‐like activity. Transcriptional analysis displayed that degQ expression was induced under heat treatment. Our results contribute to understanding the function and expression of DegQ of Xcc for the first time and provide a novel perspective about HtrA family proteins in plant pathogen.
Identifying low cancer‐specific mortality risk lymph node‐positive radical prostatectomy patients
Authors: Barletta Francesco , Tappero Stefano , Morra Simone , Incesu Reha‐Baris , Cano Garcia Cristina , Piccinelli Mattia L. , Scheipner Lukas , Tian Zhe , Gandaglia Giorgio , Stabile Armando , Mazzone Elio , Terrone Carlo , Longo Nicola , Tilki Derya , Chun Felix K. H. , de Cobelli Ottavio , Ahyai Sascha , Saad Fred , Shariat Shahrokh F. , Montorsi Francesco , Briganti Alberto , Karakiewicz Pierre I. 
Published: March 2024

AbstractObjectivesTo identify low cancer‐specific mortality (CSM) risk lymph node‐positive (pN1) radical prostatectomy (RP) patients.MethodsWithin Surveillance, Epidemiology and End Results database (2010–2015) pN1 RP patients were identified. Kaplan–Meier plots and multivariable Cox‐regression (MCR) models were used. Pathological characteristics were used to identify patients at lowest CSM risk.ResultsOverall, 2197 pN1 RP patients were identified. Overall, 5‐year cancer‐specific survival (CSS) rate was 93.3%. In MCR models ISUP GG1‐2 (hazard ratio [HR]: 0.12, p < 0.001), GG3 (HR: 0.14, p < 0.001), GG4 (HR: 0.35, p = 0.002), pT2 (HR: 0.27, p = 0.012), pT3a (HR: 0.28, p = 0.003), pT3b (HR: 0.39, p = 0.009), and 1–2 positive lymph nodes (HR: 0.64, p = 0.04) independently predicted lower CSM. Pathological characteristics subgroups with the most protective hazard ratios were used to identify low‐risk (ISUP GG1‐3 and pT2–3a and 1–2 positive lymph nodes) patients versus others (ISUP GG4‐5 or pT3b–4 or ≥3 positive lymph nodes). In Kaplan–Meier analyses, 5‐year CSS rates were 99.3% for low‐risk (n = 480, 21.8%) versus 91.8% (p < 0.001) for others (n = 1717, 78.2%).ConclusionsLymph node‐positive RP patients exhibit variable CSS rates. Within this heterogeneous group, those at very low risk of CSM may be identified based on pathological characteristics, namely ISUP GG1‐3, pT2–3a, and 1–2 positive lymph nodes. Such stratification scheme might be of value for individual patients counseling, as well as in design of clinical trials.
Three‐dimensional computed tomography‐based resection process map for robot‐assisted partial nephrectomy: propensity score matching of a single‐center retrospective study
Authors: Okada Atsushi , Ohashi Kazuya , Hashimoto Hiroya , Ota Yuya , Sugino Teruaki , Unno Rei , Iwatsuki Shoichiro , Etani Toshiki , Taguchi Kazumi , Naiki Taku , Kurokawa Satoshi , Hamamoto Shuzo , Ando Ryosuke , Nakane Akihiro , Kawai Noriyasu , Tozawa Keiichi , Yasui Takahiro 
Published: March 2024

AbstractBackground and ObjectivesWe aimed to examine the effect of preoperative three‐dimensional (3D) computed tomography (CT)‐based resection process map (RPM) imaging on the outcomes of robot‐assisted partial nephrectomy (RAPN).MethodsWe retrospectively analyzed 177 patients (RPM group, n = 92; non‐RPM group, n = 85) who underwent this surgery between November 2012 and April 2022. Patient‐specific contrast‐enhanced CT images were used to construct an RPM, a 3D representation of the kidney showing the planned tumor resection and a 5 mm safety margin. Outcome analyses were performed using propensity score matching. The primary endpoint was the trifecta achievement rate.ResultsWe extracted 90 cases. The trifecta achievement rate showed no significant differences between the RPM (73.3%) and non‐RPM groups (73.3%). However, the RPM group had fewer Grade 3 and higher complications (0.0% vs. 13.3%, p = 0.026). The da Vinci Xi (OR 3.38, p = 0.016) and tumor diameter (OR 0.95, p = 0.013) were independent factors affecting trifecta achievement in multivariate analysis. Using RPM imaging was associated with the absence of Grade 3 and higher perioperative complications (OR 5.33, p = 0.036) in univariate analysis.ConclusionsUsing preoperative 3D CT‐based RPM images before RAPN may not affect trifecta achievement, but may reduce serious complication occurrence by providing detailed information on tumor resection.
A comparison of three different microwave systems for laparoscopic liver tumor ablation
Authors: Berber Eren , Sarioglu Ali Gunduz , Akgun Ege 
Published: March 2024

AbstractBackgroundOur aim was to perform a comparison of three current microwave ablation (MWA) systems widely used for laparoscopic liver ablations in terms of ablation kinetics and geometry of ablation zones.MethodsThis was a retrospective, institutional review board‐approved study comparing Emprint, Emprint HP, and NeuWave systems for laparoscopic liver ablation. Analyses were performed via Mann−Whitney U and χ2 tests. Continuous data are presented as median (interquartile range).ResultsFor Emprint, Emprint HP, and NeuWave groups, tumor size was 1.16 (0.8), 1.21 (0.7), and 1.27 (0.9) cm (p = 0.54). Ablation time per lesion was 7 (6), 4 (2.8), and 4 (3.3) min (p < 0.0001), yielding similar ablation zone volumes and margins. The time to first ablation bubble was 1 (0.13), 1.5 (0.85), and 0.75 (0.5) min, and total ablation times were 7 (4.4), 4 (2), and 3.5 (2.8) min (p < 0.0001). The roundness index A, B, and transverse were 0.94, 0.98, and 0.79; 0.95, 0.95, and 0.78; and 1.02, 0.95, and 0.96.ConclusionsAlthough a saline‐cooling system with Emprint system allowed for larger diameter spherical ablation zones to be created, it led to decreased efficiency compared to the CO2‐cooled NeuWave system, which exposes the active antenna directly to tissue. Increased power delivered by Emprint HP improved the efficiency of saline‐cooled design, as demonstrated by faster ablation times.
Improving access to evidence‐based interventions for trauma‐exposed adults in low‐ and middle‐income countries
Authors: Kaminer Debra , Booysen Duane , Ellis Kate , Kristensen Christian Haag , Patel Anushka R. , Robjant Katy , Sardana Srishti 
Published: March 2024

AbstractIn low‐ and middle‐income countries (LMICs), the mental health consequences of trauma exposure pose a substantial personal, societal, and economic burden. Yet, the significant need for evidence‐based mental health treatment remains largely unmet. To unlock the potential for mental health care for trauma survivors in lower‐resource contexts, it is critical to map treatment barriers and identify strategies to improve access to evidence‐based, culturally appropriate, and scalable interventions. This review, based on an International Society for Traumatic Stress (ISTSS) briefing paper, describes the treatment gap facing adults with traumatic stress in LMICs and identifies the barriers that contribute to this gap. We then highlight strategies for enhancing access to effective treatments for these populations, including task‐sharing, the use of culturally adapted and multiproblem interventions, and digital tools to scale access to appropriate care. Finally, we offer recommendations for policymakers, researchers, and service providers to guide an agenda for action to close the treatment gap for trauma survivors in LMICs.
Infection in osteotomy around the knee: Incidence, management and outcomes in a high‐volume case series
Authors: Hancock Graeme , Martin Rebecca , Bell Lucy , Broderick James , Dawson Matt 
Published: March 2024

AbstractPurposeCurrent evidence around the management of osteotomy‐related infection is insufficient to robustly underpin the expert statements formulated by a recent European consensus statement. We present a review of a large case series in a high‐volume osteotomy practice to contribute to the understanding of the incidence, management and outcome of infection in this subspecialty area.MethodsAnalyses of two prospectively collected databases for all osteotomy around the knee and infections related to osteotomy were performed, along with a review of hospital readmission data to capture all osteotomy‐related infections. Clinical notes were reviewed to assess patient demographics, incidence of infection, how infection was managed and clinical outcome.ResultsIn a series of 822 osteotomies in 755 patients, there were 21 (2.8%) cases of suspected infection. Twelve (1.6%) were contemporaneously deemed ‘superficial’ and nine confirmed ‘deep’ infections (1.2%). Deep infections were all successfully managed with wound debridement, with or without plate removal, depending on union and time from initial surgery. One of these infections was noted during a revision procedure, but no revision was carried out as a direct result of infection, no external fixation was required and no infected nonunions were experienced.ConclusionAll of the cases in this series were managed successfully with debridement ± removal of the plate, without the need for revision or external fixation. Any potential signs of infection around an osteotomy, especially in the case of medial high tibial osteotomy, should raise awareness for deep infection and the need for further surgery due to the limited overlying soft tissue cover. This evidence supports the recent European Society of Sports Traumatology, Knee Surgery and Arthroscopy algorithm.Level of EvidenceLevel IV, case series.
Decreased sagittal slope of the medial tibial spine and deep concavity of the lateral tibial spine are risk factors for noncontact anterior cruciate ligament injury
Authors: Wang Shenghong , Ma Jie , Tian Cong , Feng Zhiwei , Xiang Dejian , Tang Yuchen , Geng Bin , Xia Yayi 
Published: March 2024

AbstractPurposeThis study aimed to assess the relationship between the geometric features of tibial eminence and susceptibility to noncontact anterior cruciate ligament (ACL) injuries.MethodsPatients with unilateral noncontact knee injuries between 2015 and 2021 were consecutively enroled in this study. Based on knee magnetic resonance imaging (MRI) and arthroscopic visualisation, patients were categorised into the case group (ACL rupture) and control group (ACL intact). Using MRI, the geometric features of tibial eminence were characterised by measuring the sagittal slopes, depth of concavity and coronal slopes of the inclined surfaces of the tibial spines. Univariate and multivariate logistic regressions were conducted to explore independent associations between quantified geometric indices of tibial eminence and the risk of noncontact ACL injuries.ResultsThis study included 187 cases and 199 controls. A decreased sagittal slope of the medial tibial spine (MTSSS) (combined group: odds ratio [OR]: 0.87 [0.82, 0.92], p < 0.001; females: OR: 0.88 [0.80, 0.98], p = 0.020; males: OR: 0.87 [0.81, 0.93], p < 0.001) and an increased depth of concavity in the lateral tibial spine (LTSD) (combined group: OR: 1.51 [1.24, 1.85], p < 0.001; females: OR: 1.65 [1.12, 2.43], p = 0.012; males: OR: 1.44 [1.11, 1.89], p = 0.007) were independent risk factors for noncontact ACL injuries. Moreover, a steeper coronal slope of the inclined surface of the medial tibial spine was a significant predictor of noncontact ACL injuries for males (MTSCS: OR: 1.04 [1.01, 1.08], p = 0.015) but not for females.ConclusionGeometric features of tibial eminence, particularly a decreased MTSSS and an increased LTSD, were identified as independent risk factors for noncontact ACL injuries. These findings will help clinicians identify individuals at high risk of ACL injury and facilitate the development of targeted prevention strategies.Level of EvidenceLevel III.
The anteromedial retinaculum in ACL‐injured knees: An overlooked injury?
Authors: Grunenberg Ole , Gerwing Mirjam , Oeckenpöhler Simon , Peez Christian , Briese Thorben , Glasbrenner Johannes , Hägerich Luise M. , Raschke Michael J. , Kittl Christoph , Herbst Elmar 
Published: March 2024

AbstractPurposeThe purpose of this study was to retrospectively analyse the pattern of injury to the medial knee structures in anterior cruciate ligament (ACL) injured patients. It was hypothesised that anteromedial injuries would be more common than posteromedial lesions.MethodsOne hundred and twenty subjects aged 18–25 years with a primary ACL injury were included. Patients were excluded if the time between injury and magnetic resonance imaging (MRI) was more than 28 days or if a knee dislocation or fracture was present. The MRIs were analysed with particular emphasis on injuries to the medial knee structures, menisci and bone bruise patterns. Injuries to the ligaments and anteromedial retinaculum (AMR) were graded according to severity, ranging from periligamentous oedema (grade I), partial fibre disruption of less or more than 50% (grade IIa or IIb) to complete tears (grade III).ResultsAMR injury was seen in 87 subjects (72.5%) on the coronal plane and in 88 (73.3%) on the axial plane, with grade III lesions observed in 27 (22.5%) and 29 knees (24.2%). Injuries to the superficial medial collateral ligament (sMCL), deep MCL (dMCL) and posterior oblique ligament (POL) were detected in 60 patients (50%), 93 patients (77.5%) and 38 patients (31.6%). However, grade III injuries to the POL were observed in only seven knees (5.8%). Medial meniscus injuries were associated with lesions of the sMCL and AMR (p < 0.05), while lateral meniscus injuries were significantly more common in patients with dMCL rupture (p < 0.05).ConclusionData from this study suggest that injuries to the AMR are much more common than posteromedial lesions in subjects with ACL injuries.Level of EvidenceLevel IV.
Detecting climate‐related shifts in lakes: A review of the use of satellite Earth Observation
Authors: Calamita Elisa , Lever J. Jelle , Albergel Clement , Woolway R. Iestyn , Odermatt Daniel 
Published: March 2024

AbstractClimate change exerts a profound impact on lakes, eliciting responses that range from gradual to abrupt transitions. When reaching critical tipping points, the established lake dynamics stand to undergo substantial modifications, setting off a chain reaction that reverberates through the entire ecosystem. This lake shift ripples into related ecosystem services and even influences the well‐being of human communities. Despite the importance of lake shifts, we lack a systematic overview of their occurrence, mainly due to the lack of systematic data at the global scale. We reviewed the literature focusing on climate‐related lake shifts and assessed how satellite Earth Observation (EO) has contributed to the research topic, and what we can unlock from this novel data. Our results show that EO data are used in only 9% of studies on lake shifts, although this fraction has increased since 2012. EO data is most commonly used to assess shifts in surface extent, ice coverage, or phytoplankton phenology. These variables are directly observable and the spatio‐temporal resolution of EO satellites is of great advantage. But lake shifts can also be identified indirectly from EO data, as in the example of the vertical mixing of lake water, which can be described on the basis of surface patterns. In all possible applications, we expect increasing use of EO satellites in the future, including the development of early warning systems that promise to provide timely alerts regarding impending lake shifts, thus serving as a vanguard against abrupt alterations that could ripple through interconnected ecosystem services.
Impact of Hybrid Electromagnetic Surface Modes on the Formation of Low Spatial Frequency LIPSS: A Universal Approach
Authors: Perrakis George , Tsilipakos Odysseas , Tsibidis George D. , Stratakis Emmanuel 
Published: March 2024

AbstractIn this article, the impact of the excited electromagnetic surface modes in a comprehensive investigation of the formation of laser‐induced periodic surface structures (LIPSS) is analyzed. It is demonstrated that the electromagnetic origin of low spatial‐frequency LIPSS (LSFL) is the frequency detuning between propagating and localized modes due to their coupling/hybridization. The influence of the pattern profile, inhomogeneity, and material type on the coupling strength, electric‐field spatial distribution, and associated near‐field scattering are highlighted. Exploiting the potential of the approach, evidence of a universal manifestation of LSFLs is provided irrespective of the material and the authors are able to predict and validate the experimentally‐proven lower limit of LSFL periodicity (i.e., λL/2, where λL stands for the laser wavelength). Furthermore, the analysis of the electromagnetic modes predicts that the periodicity of LSFL is practically unaffected by the laser fluence, while a suppression of LSFL at high excitation levels or large number of pulses is also predicted. It is also shown that plasmonic‐active materials are not necessary for LSFL formation perpendicular to polarization. Toward these directions, an important generic metric, namely the resonance quality factor, is additionally inserted. The approach can, thus, serve as a guide for controlling laser‐induced surface topography.
Ergodic properties of multiplication and weighted composition operators on spaces of holomorphic functions
Authors: Santacreu Daniel 
Published: March 2024

AbstractWe obtain different results about the mean ergodicity of weighted composition operators when acting on the spaces , , and , where is the open unit ball of a Banach space, as well as about the compactness and the mean ergodicity of the multiplication operator. This study relates these properties of the operators with properties of the symbol and the weight defining such operators.
ANO10‐Related Spinocerebellar Ataxia: MDSGene Systematic Literature Review and a Romani Case Series
Authors: Milovanović Andona , Westenberger Ana , Stanković Iva , Tamaš Olivera , Branković Marija , Marjanović Ana , Laabs Björn‐Hergen , Brand Max , Rajalingam Rajasumi , Marras Connie , Lohmann Katja , Branković Vesna , Novaković Ivana , Petrović Igor , Svetel Marina , Klein Christine , Kostić Vladimir S. , Dragašević‐Mišković Natasa 
Published: March 2024

AbstractBackgroundBiallelic pathogenic variants in the ANO10 gene cause autosomal recessive progressive ataxia (ATX‐ANO10).MethodsFollowing the MDSGene protocol, we systematically investigated genotype–phenotype relationships in ATX‐ANO10 based on the clinical and genetic data from 82 published and 12 newly identified patients.ResultsMost patients (>80%) had loss‐of‐function (LOF) variants. The most common variant was c.1150_1151del, found in all 29 patients of Romani ancestry, who had a 14‐year earlier mean age at onset than patients homozygous for other LOF variants. We identified previously undescribed clinical features of ATX‐ANO10 (e.g., facial muscle involvement and strabismus) suggesting the involvement of brainstem pathology, and we propose a diagnostic algorithm that may aid clinical ATX‐ANO10 diagnosis.ConclusionsThe early disease onset in patients with c.1150_1151del may indicate the existence of genetic/environmental disease‐modifying factors in the Romani population. Our findings will inform patient counseling and may improve our understanding of the disease mechanism. © 2024 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Reduced cross‐scanner variability using vendor‐agnostic sequences for single‐shell diffusion MRI
Authors: Liu Qiang , Ning Lipeng , Shaik Imam Ahmed , Liao Congyu , Gagoski Borjan , Bilgic Berkin , Grissom William , Nielsen Jon‐Fredrik , Zaitsev Maxim , Rathi Yogesh 
Published: March 2024

AbstractPurposeTo reduce the inter‐scanner variability of diffusion MRI (dMRI) measures between scanners from different vendors by developing a vendor‐neutral dMRI pulse sequence using the open‐source vendor‐agnostic Pulseq platform.MethodsWe implemented a standard EPI based dMRI sequence in Pulseq. We tested it on two clinical scanners from different vendors (Siemens Prisma and GE Premier), systematically evaluating and comparing the within‐ and inter‐scanner variability across the vendors, using both the vendor‐provided and Pulseq dMRI sequences. Assessments covered both a diffusion phantom and three human subjects, using standard error (SE) and Lin's concordance correlation to measure the repeatability and reproducibility of standard DTI metrics including fractional anisotropy (FA) and mean diffusivity (MD).ResultsIdentical dMRI sequences were executed on both scanners using Pulseq. On the phantom, the Pulseq sequence showed more than a 2.5× reduction in SE (variability) across Siemens and GE scanners. Furthermore, Pulseq sequences exhibited markedly reduced SE in‐vivo, maintaining scan‐rescan repeatability while delivering lower variability in FA and MD (more than 50% reduction in cortical/subcortical regions) compared to vendor‐provided sequences.ConclusionThe Pulseq diffusion sequence reduces the cross‐scanner variability for both phantom and in‐vivo data, which will benefit multi‐center neuroimaging studies and improve the reproducibility of neuroimaging studies.
Intra‐scan RF power amplifier drift correction
Authors: Aghaeifar Ali , Bosch Dario , Heule Rahel , Williams Sydney , Ehses Philipp , Mauconduit Franck , Scheffler Klaus 
Published: March 2024

AbstractPurposeThe drift in radiofrequency (RF) power amplifiers (RFPAs) is assessed and several contributing factors are investigated. Two approaches for prospective correction of drift are proposed and their effectiveness is evaluated.MethodsRFPA drift assessment encompasses both intra‐pulse and inter‐pulse drift analyses. Scan protocols with varying flip angle (FA), RF length, and pulse repetition time (TR) are used to gauge the influence of these parameters on drift. Directional couplers (DICOs) monitor the forward waveforms of the RFPA outputs. DICOs data is stored for evaluation, allowing calculation of correction factors to adjust RFPAs' transmit voltage. Two correction methods, predictive and run‐time, are employed: predictive correction necessitates a calibration scan, while run‐time correction calculates factors during the ongoing scan.ResultsRFPA drift is indeed influenced by the RF duty‐cycle, and in the cases examined with a maximum duty‐cycle of 66%, the potential drift is approximately 41% or 15%, depending on the specific RFPA revision. Notably, in low transmit voltage scenarios, FA has minimal impact on RFPA drift. The application of predictive and run‐time drift correction techniques effectively reduces the average drift from 10.0% to less than 1%, resulting in enhanced MR signal stability.ConclusionUtilizing DICO recordings and implementing a feedback mechanism enable the prospective correction of RFPA drift. Having a calibration scan, predictive correction can be utilized with fewer complexity; for enhanced performance, a run‐time approach can be employed.