bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low blood concentration of alcohol enhances activity related to stopping failure in the right inferior frontal cortex
Authors: Shinozaki Jun , Matsumoto Hiroshi , Saito Hidekazu , Murahara Takashi , Nagahama Hiroshi , Sakurai Yuuki , Nagamine Takashi 
Published: March 2024

Abstract
This study investigated the effects of low doses of alcohol, which are acceptable for driving a car, on inhibitory control and neural processing using the stop-signal task (SST) in 17 healthy right-handed social drinkers. The study employed simultaneous functional magnetic resonance imaging and electromyography (EMG) recordings to assess behavioral and neural responses under conditions of low-dose alcohol (breath-alcohol concentration of 0.15 mg/L) and placebo. The results demonstrated that even a small amount of alcohol consumption prolonged Go reaction times in the SST and modified stopping behavior, as evidenced by a decrease in the frequency and magnitude of partial response EMG that did not result in button pressing during successful inhibitory control. Furthermore, alcohol intake enhanced neural activity during failed inhibitory responses in the right inferior frontal cortex, suggesting its potential role in behavioral adaptation following stop-signal failure. These findings suggest that even low levels of alcohol consumption within legal driving limits can greatly impact both the cognitive performance and brain activity involved in inhibiting responses. This research provides important evidence on the neurobehavioral effects of low-dose alcohol consumption, with implications for understanding the biological basis of impaired motor control and decision-making and potentially informing legal guidelines on alcohol consumption.
Prognostic implications of premature ventricular contractions and non-sustained ventricular tachycardia in light-chain cardiac amyloidosis
Authors: Chen Zhongli , Shi Anteng , Dong Hongbin , Laptseva Natallia , Chen Feng , Yang Jiandu , Guo Xiaogang , Duru Firat , Chen Keping , Chen Liang 
Published: March 2024

Abstract

Aims
Premature ventricular contractions (PVC) and non-sustained ventricular tachycardia (NSVT) are commonly observed in light chain cardiac amyloidosis (AL-CA), but their association with prognosis is still unclear. We aimed to evaluate the prognostic value of PVCs and NSVT in patients with moderate-to-advanced AL-CA.

Methods and results
We retrospectively included patients with AL-CA at modified 2004 Mayo stages II-IIIb between February 2014 and December 2020. Twenty-four-hour Holter recordings were assessed on admission. The outcomes included (i) new onset of adverse ventricular arrhythmia (VA) or sudden cardiac death (SCD) and (ii) cardiac death during follow-up. Of the 143 patients studied (60.41 ± 11.06 years, male 64.34%), 132 (92.31%) had presence of PVC, and 50 (34.97%) had NSVT on Holter. Twelve (8.4%) patients died in hospital and 131 patients were followed up (median 24.4 months), among whom 71 patients had cardiac death, and 15 underwent adverse VA/SCD. NSVT [hazard ratio (HR): 13.57, 95% confidence interval (CI): 3.06–60.18, P &lt; 0.001], log-transformed PVC counts (HR: 1.46, 95%CI: 1.15–1.86, P = 0.002) and PVC burden (HR: 1.43 95%CI:1.14–1.80, P = 0.002) were predictive of new onset of adverse VA/SCD. The highest tertile of PVC counts (HR: 2.33, 95%CI: 1.27–4.28, P = 0.006) and PVC burden (HR: 2.58, 95%CI: 1.42–4.69, P = 0.002), rather than NSVT (HR: 1.16, 95%CI: 0.67–1.98, P = 0.603), was associated with cardiac death. Higher PVC counts/burden provided incremental value on modified 2004 Mayo stage in predicting cardiac death, with C index increasing from 0.681 to 0.712 and 0.717, respectively (P values &lt;0.05).

Conclusion
PVC count, burden, and NSVT significantly correlated with adverse VA/SCD during follow-up in patients with AL-CA. Higher PVC counts/burdens added incremental value for predicting cardiac death.
Efficacy of a novel multi-enzyme feed additive on growth performance, nutrient digestibility, and gut microbiome of weanling pigs fed corn–wheat or wheat–barley-based diet
Authors: Aderibigbe Ayodeji S , Park Chan Sol , Johnson Timothy , Velayudhan Deepak E , Vinyeta Ester , Adeola Olayiwola 
Published: March 2024

Abstract
One-hundred-and-ninety-two weanling pigs (6.7 kg body weight) were used to evaluate the impact of a carbohydrases-protease enzyme complex (CPEC) on growth performance, nutrient digestibility, and gut microbiome. Pigs were assigned to one of the four dietary treatments for 42 d according to a 2 × 2 factorial arrangement of diet type (low fiber [LF] or high fiber [HF]) and CPEC supplementation (0 or 170 mg/kg diet). The LF diet was prepared as corn–wheat-based diet while the HF diet was wheat–barley-based and contained wheat middlings and canola meal. Each dietary treatment consisted of 12 replicate pens (six replicates per gender) and four pigs per replicate pen. Over the 42-d period, there was no interaction between diet type and CPEC supplementation on growth performance indices of pigs. Dietary addition of CPEC improved (P &lt; 0.05) the body weight of pigs at days 28 and 42 and the gain-to-feed ratio of pigs from days 0 to 14. During the entire experimental period, dietary CPEC supplementation improved (P &lt; 0.05) the average daily gain and gain-to-feed ratio of pigs. There were interactions between diet type and CPEC supplementation on apparent total tract digestibility (ATTD) of dry matter (DM; P &lt; 0.01), gross energy (GE; P &lt; 0.01), and neutral detergent fiber (NDF; P &lt; 0.05) at d 42. Dietary CPEC addition improved (P &lt; 0.05) ATTD of DM, GE, and NDF in the HF diets. At day 43, dietary CPEC addition resulted in improved (P &lt; 0.05) apparent ileal digestibility (AID) of NDF and interactions (P &lt; 0.05) between diet type and CPEC supplementation on AID of DM and crude fiber. Alpha diversity indices including phylogenetic diversity and observed amplicon sequence variants of fecal microbiome increased (P &lt; 0.05) by the addition of CPEC to the HF diets on day 42. An interaction (P &lt; 0.05) between diet type and CPEC addition on Bray-Curtis dissimilarity index and Unweighted UniFrac distances was observed on day 42. In conclusion, CPEC improved weanling pig performance and feed efficiency, especially in wheat–barley diets, while dietary fiber composition had a more significant impact on fecal microbial communities than CPEC administration. The results of this study underscores carbohydrase’s potential to boost pig performance without major microbiome changes.
Eosinophil activation during immune responses: an ultrastructural view with an emphasis on viral diseases
Authors: Melo Rossana C N , Silva Thiago P 
Published: March 2024

Abstract
Eosinophils are cells of the innate immune system that orchestrate complex inflammatory responses. The study of the cell biology of eosinophils, particularly associated with cell activation, is of great interest to understand their immune responses. From a morphological perspective, activated eosinophils show ultrastructural signatures that have provided critical insights into the comprehension of their functional capabilities. Application of conventional transmission electron microscopy in combination with quantitative assessments (quantitative transmission electron microscopy), molecular imaging (immunoEM), and 3-dimensional electron tomography have generated important insights into mechanisms of eosinophil activation. This review explores a multitude of ultrastructural events taking place in eosinophils activated in vitro and in vivo as key players in allergic and inflammatory diseases, with an emphasis on viral infections. Recent progress in our understanding of biological processes underlying eosinophil activation, including in vivo mitochondrial remodeling, is discussed, and it can bring new thinking to the field.
The ecto-enzyme CD38 modulates CD4T cell immunometabolic responses and participates in HIV pathogenesis
Authors: Díaz-Basilio Fernando , Vergara-Mendoza Moisés , Romero-Rodríguez Jessica , Hernández-Rizo Sharik , Escobedo-Calvario Alejandro , Fuentes-Romero Luis-León , Pérez-Patrigeon Santiago , Murakami-Ogasawara Akio , Gomez-Palacio María , Reyes-Terán Gustavo , Jiang Wei , Vázquez-Pérez Joel-Armando , Marín-Hernández Álvaro , Romero-Rodríguez Dámaris-Priscila , Gutiérrez-Ruiz María-Concepción , Viveros-Rogel Mónica , Espinosa Enrique 
Published: March 2024

Abstract
Despite abundant evidence correlating T cell CD38 expression and HIV infection pathogenesis, its role as a CD4T cell immunometabolic regulator remains unclear. We find that CD38's extracellular glycohydrolase activity restricts metabolic reprogramming after T cell receptor (TCR)–engaging stimulation in Jurkat T CD4 cells, together with functional responses, while reducing intracellular nicotinamide adenine dinucleotide and nicotinamide mononucleotide concentrations. Selective elimination of CD38's ectoenzyme function licenses them to decrease the oxygen consumption rate/extracellular acidification rate ratio upon TCR signaling and to increase cycling, proliferation, survival, and CD40L induction. Pharmacological inhibition of ecto-CD38 catalytic activity in TM cells from chronic HIV-infected patients rescued TCR-triggered responses, including differentiation and effector functions, while reverting abnormally increased basal glycolysis, cycling, and spontaneous proinflammatory cytokine production. Additionally, ecto-CD38 blockage normalized basal and TCR-induced mitochondrial morphofunctionality, while increasing respiratory capacity in cells from HIV+ patients and healthy individuals. Ectoenzyme CD38's immunometabolic restriction of TCR-involving stimulation is relevant to CD4T cell biology and to the deleterious effects of CD38 overexpression in HIV disease.
AMPK activator 991 specifically activates SnRK1 and thereby affects seed germination in rice
Authors: Hu Yuxiang , Lin Yan , Bai Jiaqi , Xu Xuemei , Wang Ziteng , Ding Chengqiang , Ding Yanfeng , Chen Lin 
Published: March 2024

Abstract
Sucrose non-fermenting-1-related protein kinase 1 (SnRK1) and AMP-activated protein kinase (AMPK) are highly conserved. Compound 991 is an AMPK activator in mammals. However, whether 991 also activates SnRK1 remains unknown. The addition of 991 significantly increased SnRK1 activity in desalted extracts from germinating rice seeds in vitro. To determine whether 991 has biological activity, rice seeds were treated with different concentrations of 991. Germination was promoted at low concentrations but inhibited at high concentrations. The effects of 991 on germination were similar to those of OsSnRK1a overexpression. To explore whether 991 affects germination by specifically affecting SnRK1, germination of an snrk1a mutant and the wild type under 1 μM 991 treatment was compared. The snrk1a mutant was insensitive to 991. Phosphoproteomic analysis showed that the differential phosphopeptides induced by 991 and OsSnRK1a overexpression largely overlapped. Furthermore, SnRK1 might regulate rice germination in a dosage-dependent manner by regulating the phosphorylation of three phosphosites, namely S285-PIP2;4, S1013-SOS1, and S110-ABI5. These results indicate that 991 is a specific SnRK1 activator in rice. The promotion and inhibition of germination by 991 also occurred in wheat seeds. Thus, 991 is useful for exploring SnRK1 function and the chemical regulation of growth and development in crops.
Single-cell transcriptomics reveals heterogeneity in plant responses to the environment: a focus on biotic and abiotic interactions
Authors: Tenorio Berrío Rubén , Dubois Marieke 
Published: March 2024

Abstract
Biotic and abiotic environmental cues are major factors influencing plant growth and productivity. Interactions with biotic (e.g. symbionts and pathogens) and abiotic (e.g. changes in temperature, water, or nutrient availability) factors trigger signaling and downstream transcriptome adjustments in plants. While bulk RNA-sequencing technologies have traditionally been used to profile these transcriptional changes, tissue homogenization may mask heterogeneity of responses resulting from the cellular complexity of organs. Thus, whether different cell types respond equally to environmental fluctuations, or whether subsets of the responses are cell-type specific, are long-lasting questions in plant biology. The recent breakthrough of single-cell transcriptomics in plant research offers an unprecedented view of cellular responses under changing environmental conditions. In this review, we discuss the contribution of single-cell transcriptomics to the understanding of cell-type-specific plant responses to biotic and abiotic environmental interactions. Besides major biological findings, we present some technical challenges coupled to single-cell studies of plant–environment interactions, proposing possible solutions and exciting paths for future research.
Flowering time genes branching out
Authors: Colleoni Pierangela E , van Es Sam W , Winkelmolen Ton , Immink Richard G H , van Esse G Wilma 
Published: March 2024

Abstract
Plants are sessile by nature, and as such they have evolved to sense changes in seasonality and their surrounding environment, and adapt to these changes. One prime example of this is the regulation of flowering time in angiosperms, which is precisely timed by the coordinated action of two proteins: FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1). Both of these regulators are members of the PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN (PEBP) family of proteins. These regulatory proteins do not interact with DNA themselves, but instead interact with transcriptional regulators, such as FLOWERING LOCUS D (FD). FT and TFL1 were initially identified as key regulators of flowering time, acting through binding with FD; however, PEBP family members are also involved in shaping plant architecture and development. In addition, PEBPs can interact with TCP transcriptional regulators, such as TEOSINTE BRANCHED 1 (TB1), a well-known regulator of plant architecture, and key domestication-related genes in many crops. Here, we review the role of PEBPs in flowering time, plant architecture, and development. As these are also key yield-related traits, we highlight examples from the model plant Arabidopsis as well as important food and feed crops such as, rice, barley, wheat, tomato, and potato.
Particle initialization effects on Lyman-α forest statistics in cosmological SPH simulations
Authors: Khan Nabendu Kumar , Kulkarni Girish , Bolton James S , Haehnelt Martin G , Iršič Vid , Puchwein Ewald , Asthana Shikhar 
Published: March 2024

ABSTRACT
Confronting measurements of the Lyman-α forest with cosmological hydrodynamical simulations has produced stringent constraints on models of particle dark matter and the thermal and ionization state of the intergalactic medium. We investigate the robustness of such models of the Lyman-α forest, focusing on the effect of particle initial conditions on the Lyman-α forest statistics in cosmological SPH simulations. We study multiple particle initialization algorithms in simulations that are designed to be identical in other respects. In agreement with the literature, we find that the correct linear theory evolution is obtained when a glass-like configuration is used for initial unperturbed gas particle positions alongside a regular grid configuration for dark matter particles and the use of non-identical initial density perturbations for gas and dark matter. However, we report that this introduces a large scale-dependent distortion in the 1D Lyman-α transmission power spectrum at small scales (k &gt; 0.05 s km−1). The effect is close to 50 per cent at k ∼ 0.1 s km−1, and persists at higher resolution. This can severely bias inferences in parameters such as the dark matter particle mass. By considering multiple initial conditions codes and their variations, we also study the impact of a variety of other assumptions and algorithmic choices, such as adaptive softening, background radiation density, particle staggering, and perturbation theory accuracy, on the matter power spectrum, the Lyman-α flux power spectrum, and the Lyman-α flux PDF. This work reveals possible pathways towards more accurate theoretical models of the Lyman-α forest to match the quality of upcoming measurements.
Local models of two-temperature accretion disc coronae – II. Ion thermal conduction and the absence of disc evaporation
Authors: Bambic Christopher J , Quataert Eliot , Kunz Matthew W , Jiang Yan-Fei 
Published: March 2024

ABSTRACT
We use local stratified shearing-box simulations with magnetic field-aligned thermal conduction to study an idealized model of the coupling between a cold, radiatively efficient accretion disc, and an overlying, hot, two-temperature corona. Evaporation of a cold disc by conduction from the hot corona has been proposed as a means of mediating the soft-to-hard state transitions observed in X-ray binary systems. We model the coronal plasma in our local disc patch as an MHD fluid subject to both free-streaming ion conduction and a parametrized cooling function that captures the collisional transfer of energy from hot ions to colder, rapidly cooling leptons. In all of our models, independent of the initial net vertical magnetic flux (NF) threading the disc, we find no evidence of disc evaporation. The ion heat flux into the disc is radiated away before conduction can heat the disc’s surface layers. When an initial NF is present, steady-state temperature, density, and outflow velocities in our model coronae are unaffected by conduction. Instead of facilitating disc evaporation, thermal conduction is more likely to feed the disc with plasma condensing out of the corona, particularly in flows without NF. Our work indicates that uncertainties in the amount of NF threading the disc hold far greater influence over whether or not the disc will evaporate into a radiatively inefficient accretion flow compared to thermal conduction. We speculate that a change in net flux mediates disc truncation/evaporation.
Long-term double synchronization in close-in gas giant planets
Authors: Guo Shuaishuai , Guo Jianheng , Su Jie , Yan Dongdong 
Published: March 2024

ABSTRACT
Hot Jupiters, orbiting their host stars at extremely close distances, undergo tidal evolution, with some being engulfed by their stars due to angular momentum exchanges induced by tidal forces. However, achieving double synchronization can prolong their survival. Using the mesa stellar evolution code, combined with the magnetic braking model of Matt et al. (2015), we calculate 25 000 models with different metallicity and study how to attain the conditions that trigger the long-term double synchronization. Our results indicate that massive planets orbiting stars with lower convective turnover time are easier to achieve long-term double synchronization. The rotation angular velocity at the equilibrium point (Ωsta) is almost equal to orbital angular velocity of planet (n) for the majority of the main sequence lifetime if a system has undergone a long-term double synchronization, regardless of their state at this moment. We further compared our results with known parameters of giant planetary systems and found that those systems with larger planetary masses and lower convective turnover time seem to be less sensitive to changes in the tidal quality factor $Q^{\prime }_{_*}$. We suggest that for systems that fall on the state of Ωsta ≈ n, regardless of their current state, the synchronization will persist for a long time if orbital synchronization occurs at any stage of their evolution. Our results can be applied to estimate whether a system has experienced long-term double synchronization in the past or may experience it in the future.
White dwarf pollution: one star or two?
Authors: Noor Hiba Tu , Farihi Jay , Hollands Mark , Toonen Silvia 
Published: March 2024

ABSTRACT
The accretion of tidally disrupted planetary bodies is the current consensus model for the presence of photospheric metals commonly detected in white dwarfs. While most dynamical studies have considered a single star and associated planetary instabilities, several investigations have instead considered the influence of widely bound stellar companions as potential drivers of white dwarf pollution. This study examines the prevalence of wide binaries among polluted white dwarfs using Gaia Data Release 3 astrometry, where three samples are investigated: 71 DAZ stars with metals detected in the ultraviolet using Hubble Space Telescope, and two groups of DZ stars identified via Sloan Digital Sky Survey spectroscopy, comprised of 116 warmer and 101 cooler sources. Each sample was searched for spatially resolved, comoving companions, and compared to the same analysis of thousands of field white dwarfs within overlapping regions of the Gaia Hertzsprung–Russell diagram. The wide binary fraction of the DAZ sample is $10.6_{-3.2}^{+3.9}$ per cent, and within $1\sigma$ of the corresponding field. However, the search yields wide binary fractions of less than 1.8 per cent for the two independent DZ star catalogues, which are each distinct from their fields by more than $3\sigma$. Both sets of results support that pollution in white dwarfs is not the result of stellar companions, and the delivery of metals to white dwarf surfaces is caused by major planets. The discrepancy between the DAZ and DZ star wide binary fractions cannot be caused by white dwarf spectral evolution, suggesting these two populations may have distinct planetary architectures.
Comprehensive analysis of MYB/MYBL1-altered pediatric-type diffuse low-grade glioma
Authors: Moreira Daniel C , Qaddoumi Ibrahim , Spiller Susan , Bouldin Thomas W , Davidson Alan , Saba-Silva Nasjla , Sullivan Daniel V , Tanaka Ryuma , Wagner Aaron S , Wood Matthew , Klimo Paul , Job Godwin , Devidas Meenakshi , Li Xiaoyu , Gajjar Amar , Robinson Giles W , Chiang Jason 
Published: March 2024

Abstract

Background
Pediatric-type diffuse low-grade gliomas (pLGG) harboring recurrent genetic alterations involving MYB or MYBL1 are closely related tumors. Detailed treatment and outcome data of large cohorts are still limited. This study aimed to comprehensively evaluate pLGG with these alterations to define optimal therapeutic strategies.

Methods
We retrospectively reviewed details of pLGG with MYB or MYBL1 alterations from patients treated or referred for pathologic review at St. Jude Children’s Research Hospital. Tumor specimens were centrally reviewed, and clinical data were collated.

Results
Thirty-three patients (18 male; median age, 5 years) were identified. Two tumors had MYBL1 alterations; 31 had MYB alterations, MYB::QKI fusion being the most common (n = 10, 30%). Most tumors were in the cerebral hemispheres (n = 22, 67%). Two patients (6%) had metastasis at diagnosis. The median follow-up was 6.1 years. The 5-year event-free survival (EFS) rate was 81.3% ± 8.3%; the 5-year overall survival (OS) rate was 96.4% ± 4.1%. Patients receiving a near-total or gross-total resection had a 5-year EFS of 100%; those receiving a biopsy or subtotal resection had a 5-year EFS rate of 56.6% ± 15.2% (P &lt; .01). No difference in EFS was observed based on location, histology, or molecular alterations. However, the tumors that progressed or metastasized may have distinct methylation profiles with evidence of activation of the MAPK and PI3K/AKT/mTOR pathways.

Conclusions
pLGG with MYB/MYBL1 alterations have good outcomes. Our findings suggest that surgical resectability is a crucial determinant of EFS. Further characterization is required to identify optimal treatment strategies for progressive tumors.