bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring functional genes’ correlation with (
S
)-equol concentration and new daidzein racemase identification
Authors: Hu Yun-Fei , Luo Shu , Wang Sheng-Qi , Chen Ke-Xin , Zhong Wei-Xuan , Li Bai-Yuan , Cao Lin-Yan , Chen Hua-Hai , Yin Ye-Shi 
Published: March 2024

ABSTRACT

With its estrogenic activity, (
S
)-equol plays an important role in maintaining host health and preventing estrogen-related diseases. Exclusive production occurs through the transformation of soy isoflavones by intestinal bacteria, but the reasons for variations in (
S
)-equol production among different individuals and species remain unclear. Here, fecal samples from humans, pigs, chickens, mice, and rats were used as research objects. The concentrations of (
S
)-equol, along with the genetic homology and evolutionary relationships of (
S
)-equol production-related genes [daidzein reductase (DZNR), daidzein racemase (DDRC), dihydrodaidzein reductase (DHDR), tetrahydrodaidzein reductase (THDR)], were analyzed. Additionally,
in vitro
functional verification of the newly identified DDRC gene was conducted. It was found that approximately 40% of human samples contained (
S
)-equol, whereas 100% of samples from other species contained (
S
)-equol. However, there were significant variations in (
S)-
equol content among the different species: rats > pigs > chickens > mice > humans. The distributions of the four genes displayed species-specific patterns. High detection rates across various species were exhibited by
DHDR
,
THDR
, and
DDRC
. In contrast, substantial variations in detection rates among different species and individuals were observed with respect to
DZNR
. It appears that various types of
DZNR
may be associated with different concentrations of (
S)-
equol, which potentially correspond to the regulatory role during (
S)-
equol synthesis. This enhances our understanding of individual variations in (
S
)-equol production and their connection with functional genes
in vitro
. Moreover, the newly identified DDRC exhibits higher potential for (
S)-
equol synthesis compared to the known DDRC, providing valuable resources for advancing
in vitro
(
S)-
equol production.

IMPORTANCE

(
S
)-equol ((
S
)-EQ) plays a crucial role in maintaining human health, along with its known capacity to prevent and treat various diseases, including cardiovascular diseases, metabolic syndromes, osteoporosis, diabetes, brain-related diseases, high blood pressure, hyperlipidemia, obesity, and inflammation. However, factors affecting individual variations in (
S
)-EQ production and the underlying regulatory mechanisms remain elusive. This study examines the association between functional genes and (
S
)-EQ production, highlighting a potential correlation between the DZNR gene and (
S
)-EQ content. Various types of DZNR may be linked to the regulation of (
S
)-EQ synthesis. Furthermore, the identification of a new DDRC gene offers promising prospects for enhancing in vitro (
S
)-EQ production.
Cathelicidin boosts the antifungal activity of neutrophils and improves prognosis during
Aspergillus fumigatus
keratitis
Authors: Hou Xiaochen , Li Cui , Liu Jingyi , Yang Shanshan , Peng Xudong , Wang Qian , Liu Chengxiu , Liu Xing , Luan Junjie , Zhao Guiqiu , Lin Jing 
Published: March 2024

ABSTRACT

Aspergillus fumigatus
(
A. fumigatus
) is one of the common pathogens of fungal keratitis. Fungal growth and invasion cause excessive inflammation and corneal damage, leading to severe vision loss. Neutrophils are the primary infiltrating cells critical for fungal clearance. Cathelicidin [LL-37 in humans and cathelicidin-related antimicrobial peptide (CRAMP) in mice], a natural antimicrobial peptide, can directly inhibit the growth of many pathogens and regulate immune responses. However, the role of cathelicidin and its effect on neutrophils in
A. fumigatus
keratitis remain unclear. By establishing
A. fumigatus
keratitis mouse models, we found that cathelicidin was increased in
A. fumigatus
keratitis. It could reduce fungal loads, lower clinical scores, and improve corneal transparency. Restriction of CRAMP on fungal proliferation was largely counteracted in

CD18
−/−

mice, in which neutrophils cannot migrate into infected sites. When WT neutrophils were transferred into

CD18
−/−

mice, corneal fungal loads were distinctly reduced, indicating that neutrophils are vital for CRAMP-mediated resistance. Furthermore, cathelicidin promoted neutrophils to phagocytose and degrade conidia both
in vitro
and
in vivo
. CXC chemokine receptor 2 (CXCR2) was reported to be a functional receptor of LL-37 on neutrophils. CXCR2 antagonist SB225002 or phospholipase C (PLC) inhibitor U73122 weakened LL-37-induced phagocytosis. Meanwhile, LL-37 induced PLC γ phosphorylation, which was attenuated by SB225002. SB225002 or the autophagy inhibitors Bafilomycin-A1 and 3-Methyladenine weakened LL-37-induced degradation of conidia. Transmission electron microscopy (TEM) observed that LL-37 increased autophagosomes in
Aspergillus
-infected neutrophils. Consistently, LL-37 elevated autophagy-associated protein expressions (Beclin-1 and LC3-II), but this effect was weakened by SB225002. Collectively, cathelicidin reduces fungal loads and improves the prognosis of
A. fumigatus
keratitis. Both
in vitro
and
in vivo
, cathelicidin promotes neutrophils to phagocytose and degrade conidia. LL-37/CXCR2 activates PLC γ to amplify neutrophils′ phagocytosis and induces autophagy to eliminate intracellular conidia.
Supplemental Material: Moderate magnitude clockwise rotation of the Yunlong Basin: Implications for synchronous Eocene rotation of the southeastern Tibetan Plateau
Authors: Xu Wanlong , al. et 
Published: March 2024

<p>Figure S1: E/I inclination shallowing corrections of the ChRM directions from the studied Yunlong Formation. Table S1: Cretaceous–Oligocene paleomagnetic results from the Lanping-Simao terrane and the eastern Qiangtang terrane. Table S2: Paleomagnetic rotation results in the Gonjo, Mangkang, Lanping, Yunlong and Mengla area. </p>
Association between individual-level socioeconomic factors and intimate partner violence victimisation in women: a systematic review protocol
Authors: Reyal Haizana Parween , Dissanayake Nayomi , Gunarathna Harsha , Soysa Dilukshi , Fernando Manoj Sanjeewa , Senarathna Lalith 
Published: March 2024

IntroductionIntimate partner violence (IPV) is a global public health problem. Although both men and women experience IPV, the burden is more on women. To address IPV effectively, it is important to understand the factors that cause IPV including the socioeconomic factors. However, there is an inadequacy of knowledge on how socioeconomic factors at different levels affect IPV. Hence, the objective is to review the individual-level socioeconomic factors associated with IPV victimisation of women and girls.Methods and analysisThe search strategy was developed to identify publications from January 2010 to 30 June 2024. The selected electronic databases of PubMed/MEDLINE, EMBASE, CINAHL, PsycINFO, Web of Science, Scopus and Science Direct will be searched. The eligibility criteria for data collection are based on participants/population (women and girls), exposure (socioeconomic factors) and outcome (IPV). In primary search, the title and abstracts will be screened and reference lists of selected articles will be screened for additional studies. Two researchers will independently screen the articles, and in any disagreements, a third researcher will be consulted. The data will be tabulated to present the study and participant characteristics, comparison descriptors between victims and non-victims, inclusion and exclusion criteria, primary and secondary outcomes data, results, limitations and implications. A quality assessment will be performed on the selected studies to avoid bias. A narrative synthesis will summarise the findings.Ethics and disseminationEthical approval was waived because only secondary data are used. The protocol will be published, and the findings will be disseminated via publication in a peer-reviewed journal.PROSPERO registration numberCRD42022373535.
Redo-TAVR Feasibility After SAPIEN 3 Stratified by Implant Depth and Commissural Alignment: A CT Simulation Study
Authors: Koshy Anoop N. , Tang Gilbert H.L. , Khera Sahil , Vinayak Manish , Berdan Megan , Gudibendi Sneha , Hooda Amit , Safi Lucy , Lerakis Stamatios , Dangas George D. , Sharma Samin K. , Kini Annapoorna S. , Krishnamoorthy Parasuram 
Published: March 2024

BACKGROUND:
Redo-transcatheter aortic valve replacement (TAVR) can pin the index transcatheter heart valve leaflets open leading to sinus sequestration and restricting coronary access. The impact of initial implant depth and commissural alignment on redo-TAVR feasibility is unclear. We sought to determine the feasibility of redo-TAVR and coronary access after SAPIEN 3 (S3) TAVR stratified by implant depth and commissural alignment.

METHODS:
Consecutive patients with native valve aortic stenosis were evaluated using multidetector computed tomography. S3 TAVR simulations were done at 3 implant depths, sizing per manufacturer recommendation and assuming nominal expansion in all cases. Redo-TAVR was deemed unfeasible based on valve-to-sinotubular junction distance and valve-to-sinus height <2 mm, while the neoskirt plane of the S3 transcatheter heart valve estimated coronary access feasibility.

RESULTS:

Overall, 1900 patients (mean age, 80.2±8 years; STS-PROM [Society of Thoracic Surgeons Predicted Risk of Operative Mortality], 3.4%) were included. Redo-TAVR feasibility reduced significantly at shallower initial S3 implant depths (2.3% at 80:20 versus 27.5% at 100:0,
P
<0.001). Larger S3 sizes reduced redo-TAVR feasibility, but only in patients with a 100:0 implant (
P
<0.001). Commissural alignment would render redo-TAVR feasible in all patients, assuming the utilization of leaflet modification techniques to reduce the neoskirt height. Coronary access following TAV-in-TAV was affected by both index S3 implant depth and size.

CONCLUSIONS:
This study highlights the critical impact of implant depth, commissural alignment, and transcatheter heart valve size in predicting redo-TAVR feasibility. These findings highlight the necessity for individualized preprocedural planning, considering both immediate results and long-term prospects for reintervention as TAVR is increasingly utilized in younger patients with aortic stenosis.
The Political Strategies and Ideological Transformation of Political Conservatism: Russian Monarchist Emigrant Organisations’ Political Activity in the 1920s
Authors: Kochetova Anastasiia 
Published: March 2024

Abstract
The twentieth century may be characterised as an epoch dominated by migratory movements, marked particularly by the substantial human displacements precipitated by wars and revolutionary upheavals during its first half. Historiographical tendencies have often led to a narrowed concentration on select dimensions of political immigration, neglecting the broader spectrum of ideological currents that influenced these movements. A case in point is the insufficiently examined narrative of Russian monarchist factions in Western Europe during the 1920s and early 1930s, and the subsequent evolution or dissolution of their ideological tenets. This research undertakes a historiographical analysis, a comprehensive review of both domestic and international scholarly literature, and a thorough evaluation of ancillary sources concerning the activities of Russian monarchist entities in Western Europe throughout the 1920s and 1930s. The study specifically accentuates the chronological segment of the 1920s within the history of the Russian monarchist movement, illuminating their concerted efforts to formulate a political doctrine that not only aimed at the reinstatement of monarchical governance but also sought to present a counter-narrative to the communist manifesto propagated by the Bolsheviks, thereby attempting to establish a more compelling and pragmatic alternative.
Mental health messages on TikTok: Analysing the use of emotional appeals in health-related #EduTok videos
Authors: Jerin Sultana Ismet , O’Donnell Nicole , Mu Di 
Published: March 2024

Objective: This study aimed to evaluate how health educators use emotional appeals on the social media site TikTok to promote well-being, engage audiences and reduce mental health stigma. Design/Method: Our research team conducted a quantitative content analysis of 400 TikTok videos centred on health education. We evaluated the frequency of mental health content compared with general health education content and associated views, engagement and use of emotional appeals. Results: Mental health content frequently appeared in #EduTok health videos and earned significantly greater reach than general health content. However, videos discussing mental health earned less engagement and relied less on emotional appeals. Mental health content mainly relied on affiliation, hope and sorrow message appeals, although the presence of these appeals did not significantly affect engagement. General health content utilised personal stories, expert credentials and emotional appeals more frequently than mental health content. Conclusion: Evidence from this research suggests that mental health educational content underperforms general health videos and underutilises emotional appeals on TikTok. Health educators must critically analyse and adjust message strategies to enhance audience reception, taking note of success across health topics. We discuss the implications for how practitioners can use TikTok to reduce stigma and promote positive mental health with emotionally engaging content, especially among young audiences.
Envisioning Emerging Research  Trends on Impact of COVID-19 on Indian Economy: A Bibliometric  Study Applying Latent Semantic Analysis and Text Mining Technique
Authors: Misra Pooja , Gupta Jaya 
Published: March 2024

The COVID-19 pandemic ignited an unprecedented emergency for countries across the globe. The twin threats to lives and livelihood caused due to the pandemic brought severe ramifications to the Indian economy. The labour market faced the brunt exponentially, and the same is evident from the extensive research studies undertaken in this realm. A large volume of research has been published in this domain analysing the impact of COVID-19. This bibliometric study presents the dominant areas of current research upon analysing the extant literature on the economic impact of the pandemic. The study identifies prevalent topics and themes mapped from the existing knowledge base by applying natural language processing (NLP) and machine learning. The study proposes areas for further research. JEL Codes: I1, I3, E7 1, E2
Profile of Chinese Cluster Headache Register Individual Study (CHRIS): Clinical characteristics, diagnosis and treatment status data of 816 patients in China
Authors: Zhang Shuhua , Xu Suiyi , Chen Chunfu , Xue Zhanyou , Yao Yuanrong , Zhao Hongru , Zhao Hongling , Ji Yabin , Wang Dan , Hu Dongmei , Liu Kaiming , Chen Jianjun , Chen Sufen , Gao Xiaoyu , Gui Wei , Fan Zhiliang , Wan Dongjun , Yuan Xueqian , Qu Wensheng , Xiao Zheman , Dong Ming , Wang Hebo , Ju Chunyang , Xu Huifang , Zhang Liang , Wang Xiaolin , Zhang Mingjie , Han Xun , Ran Ye , Jia Zhihua , Su Hui , Li Yingji , Liu Huanxian , Zhao Wei , Gong Zihua , Lin Xiaoxue , Liu Yingyuan , Sun Yin , Xie Siyuan , Zhai Deqi , Liu Ruozhuo , Wang Shengshu , Dong Zhao , Yu Shengyuan ,   on behalf of the Chinese Cluster Headache Alliance (CCHA), Liu Zhonglin , Wang Yanhong , Li Li , Wang Zhe , Mei Li , Xu Peng , Yu Haiyan , Liu Yang , Chen Min , Liu Rui , He Weiliang , Yang Jiajun , Tan Ge , Li Jiasi , Zhang Dan , Li Xianliang , Man Yuhong , Li Lin , He Yusheng , Ren Zhanxiu , Wang Xiaojuan , Zhang Jie , An Xingkai , Zhang Zhongling , Zhang Jing , Liu Yonghui , Zhang Yi , Gu Qun , Wang Lijuan , Qiu Shi , Cai Haiyan , Yan Lanyun , Hu Nana , Huang Chun , Yin Mei , Zhang Ran , Yi Juan , Chen Fang , Gao Qiaolin , Wang Yi , Zhang Zengqiang , Qin Yunqing , Hao Yu , Zhang Qi , Song Yongbin , Xue Hui , Feng Peng , Shao Wei , Li Mingxin , Huang Chenmin , Chen Weian , Tong Qiuling 
Published: March 2024

Background The clinical profile of cluster headache may differ among different regions of the world, warranting interest in the data obtained from the initial Chinese Cluster Headache Register Individual Study (CHRIS) for better understanding. Methods We conducted a multicenter, prospective, longitudinal cohort study on cluster headache across all 31 provinces of China, aiming to gather clinical characteristics, treatment approaches, imaging, electrophysiological and biological samples. Results In total 816 patients were enrolled with a male-to-female ratio of 4.33:1. The mean age at consultation was 34.98 ± 9.91 years, and 24.89 ± 9.77 years at onset. Only 2.33% were diagnosed with chronic cluster headache, and 6.99% had a family history of the condition. The most common bout was one to two times per year (45.96%), lasting two weeks to one month (44.00%), and occurring frequently in spring (76.23%) and winter (73.04%). Of these, 68.50% experienced one to two attacks per day, with the majority lasting one to two hours (45.59%). The most common time for attacks was between 9 am and 12 pm (75.86%), followed by 1 am and 3 am (43.48%). Lacrimation (78.80%) was the most predominant autonomic symptom reported. Furthermore, 39.22% of patients experienced a delay of 10 years or more in receiving a correct diagnosis. Only 35.67% and 24.26% of patients received common acute and preventive treatments, respectively. Conclusion Due to differences in ethnicity, genetics and lifestyle conditions, CHRIS has provided valuable baseline data from China. By establishing a dynamic cohort with comprehensive multidimensional data, it aims to advance the management system for cluster headache in China.
Investigations of the migraine-provoking effect of levcromakalim in patients with migraine with aura
Authors: Thomsen Andreas Vinther , Al-Karagholi Mohammad Al-Mahdi , Hougaard Anders , Ostrowski Sisse Rye , Pedersen Ole Birger , Hansen Thomas Folkmann , Ashina Messoud 
Published: March 2024

Background/Hypothesis Experimental provocation studies have yielded important insights in migraine pathophysiology. Levcromakalim has been previously shown to induce migraine-like attacks with and without aura. In this study, we aim to further explore the migraine aura-inducing potential of levcromakalim. Methods In a double-blind, randomized, placebo-controlled cross-over study, 27 adult participants with migraine with aura received intravenous infusions of levcromakalim and saline. Headache, aura and associated symptoms were evaluated for 24 hours following administration of the study drug. The primary endpoint was occurrence of migraine-like attacks with or without aura in the 24-hour observation period. Results Thirteen participants developed migraine-like attacks on the active day only (P = 0.0098), and four participants developed aura on the active day only (P = 0.68). The median time to onset of migraine-like headache was three hours, and the median time to onset of aura was 27.5 minutes. Conclusion/Interpretation Our findings affirm the potent migraine-inducing effect of levcromakalim. We observed a lower induction-rate of migraine aura than previously reported. Further studies are warranted to identify predictors of migraine aura following levcromakalim. ClinicalTrials.gov identifier NCT04905654
Probiotics ameliorate endocrine disorders via modulating inflammatory pathways: a systematic review
Authors: Nemati Marzieh , Ebrahimi Bahareh , Montazeri-Najafabady Nima 
Published: March 2024

AbstractProbiotics has offered a new prospect to treat and manage a variety of endocrine disorders such as obesity, diabetes, non- alcoholic fatty liver disease and metabolic syndrome. The precise mechanisms by which probiotics exert their beneficial effects on endocrine disorders and its associated problems are still indecisive. It seems that regulating the immune system and suppressing pro-inflammatory pathways like tumor necrosis factor-α and interleukin-6 or triggering anti-inflammatory pathways like interleukin-4 and 10 may be one of the potential mechanisms in the managing of endocrine disorders. In this systematic review, we hypothesized that various probiotic strains (Lactobacillus, Biofidiobacteria, Streptococcus, Entrococcus, Clostridium, and Bacillus) alone or in combination with each other could manage endocrine disorders via modulating inflammatory pathways such as suppressing pro-inflammatory cytokines (IL-6, IL-12, TNF-α, TNF-β, NFκB, and MCP-1), stimulating anti-inflammatory cytokines (IL-4,IL-6, IL-22, IL-23, IL-33, and TGF-β) and maintaining other factors like C-reactive protein, Toll like receptors, LPS, and NK cells. Data source this search was performed in PubMed and Scopus. Both human and animal studies were included. Among more than 15,000 papers, 25 studies were identified as eligible for more assessments. Quality assessment of the studies was cheeked by two researchers independently by title and abstract screening, then article which have inclusion criteria were included, and data retrieved from the included full text studies as the authors had originally reported. Results specified that Lactobacillus has been the most widely used probiotic as well as which one exhibiting the extend of the therapeutic effects on endocrine disorders, especially obesity by modulating immune responses. Also, most studies have revealed that probiotics through suppressing pro-inflammatory pathways specially via reducing levels TNF-α cytokine exhibited protective or beneficial effects on endocrine diseases particularly obesity as well as through decreasing level of IL-6 induced therapeutic effects in diabetes. This systematic review suggests that probiotics could ameliorate endocrine disorders via their immunomodulatory effects.