bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy efficiency of Gaussian and ring profiles for LPBF of nickel alloy 718
Authors: Cozzolino Ersilia , Tiley Austin J. , Ramirez Antonio J. , Astarita Antonello , Herderick Edward D. 
Published: April 2024

AbstractAdditive manufacturing (AM) has the potential for improving the sustainability of metal processing through decreased energy and materials usage compared to casting and forging. Laser powder bed fusion (LPBF) of high-temperature alloys such as nickel alloy 718 is one of the key modalities supporting this effort. One of the major drawbacks to LPBF is its slow build speed on the order of 5–10 cubic centimeters per hour print speed. This experimental study investigates how to increase the productivity of the LPBF process by switching from a traditional Gaussian laser shape to a ring laser shape using a nLight multi-modal laser. The objective is to increase productivity, reducing energy consumption and time, without sacrificing mechanical properties by switching to the ring laser thereby improving the sustainability of LPBF. Results include measuring the energy consumption of an Open Additive LPBF system during 718 printing and comparing the microstructure and mechanical properties of the two different lasers.
Cymbopogon proximus and Petroselinum crispum seed ethanolic extract/Gum Arabic nanogel emulsion: Preventing ethylene glycol and ammonium chloride-induced urolithiasis in rats
Authors: Essa Hend A. , Ali Alaa M. , Saied Mona A. 
Published: April 2024

AbstractUrolithiasis is a prevalent urological disorder that contributes significantly to global morbidity. This study aimed to assess the anti-urolithic effects of Cymbopogon proximus (Halfa Bar) and Petroselinum crispum (parsley) seed ethanolic extract /Gum Arabic (GA) emulsion, and its nanogel form against ethylene glycol (EG) and ammonium chloride (AC)-induced experimental urolithiasis in rats. Rats were divided into four groups: group 1 served as the normal control, group 2 received EG with AC in drinking water for 14 days to induce urolithiasis, groups 3 and 4 were orally administered emulsion (600 mg/kg/day) and nanogel emulsion (600 mg/kg/day) for 7 days, followed by co-administration with EG and AC in drinking water for 14 days. Urolithiatic rats exhibited a significant decrease in urinary excreted magnesium, and non-enzymic antioxidant glutathione and catalase activity. Moreover, they showed an increase in oxalate crystal numbers and various urolithiasis promoters, including excreted calcium, oxalate, phosphate, and uric acid. Renal function parameters and lipid peroxidation were intensified. Treatment with either emulsion or nanogel emulsion significantly elevated urolithiasis inhibitors, excreted magnesium, glutathione levels, and catalase activities. Reduced oxalate crystal numbers, urolithiasis promoters’ excretion, renal function parameters, and lipid peroxidation while improving histopathological changes. Moreover, it decreased renal crystal deposition score and the expression of Tumer necrosis factor-α (TNF-α) and cleaved caspase-3. Notably, nanogel emulsion showed superior effects compared to the emulsion. Cymbopogon proximus (C. proximus) and Petroselinum crispum (P. crispum) seed ethanolic extracts/GA nanogel emulsion demonstrated protective effects against ethylene glycol induced renal stones by mitigating kidney dysfunction, oxalate crystal formation, and histological alterations.
A novel thermotolerant l-rhamnose isomerase variant for biocatalytic conversion of d-allulose to d-allose
Authors: Sharma Sweety , Patel Satya Narayan , Singh Sudhir P. 
Published: April 2024

Abstract
A novel l-rhamnose isomerase was identified and cloned from an extreme-temperature aquatic habitat metagenome. The deduced amino acid sequence homology suggested the possible source of this metagenomic sequence to be Chloroflexus islandicus. The gene expression was performed in a heterologous host, Escherichia coli, and the recombinant protein l-rhamnose isomerase (L-RIM) was extracted and purified. The catalytic function of L-RIM was characterized for d-allulose to d-allose bioconversion. d-Allose is a sweet, rare sugar molecule with anti-tumour, anti-hypertensive, cryoprotective, and antioxidative properties. The characterization experiments showed L-RIM to be a Co++- or Mn++-dependent metalloenzyme. L-RIM was remarkably active (~ 80%) in a broad spectrum of pH (6.0 to 9.0) and temperature (70 to 80 °C) ranges. Optimal L-RIM activity with d-allulose as the substrate occurred at pH 7.0 and 75 °C. The enzyme was found to be excessively heat stable, displaying a half-life of about 12 days and 5 days at 65 °C and 70 °C, respectively. L-RIM catalysis conducted at slightly acidic pH of 6.0 and 70 °C achieved biosynthesis of about 30 g L−1 from 100 g L−1d-allulose in 3 h.

Key points
• The present study explored an extreme temperature metagenome to identify a novel gene that encodes a thermostable l-rhamnose isomerase (L-RIM)
• L-RIMexhibits substantial (80% or more) activity in a broad spectrum of pH (6.0 to 9.0) and temperature (70 to 80 °C) ranges
• L-RIMis excessively heat stable, displaying a half-life of about 12 days and 5 days at 65 °C and 70 °C, respectively
Non-coding regulatory sRNAs from bacteria of the Burkholderia cepacia complex
Authors: Matos Gonçalo R. , Feliciano Joana R. , Leitão Jorge H. 
Published: April 2024

Abstract
Small non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Hundreds of sRNAs have been found using in silico genome analysis and experimentally based approaches in bacteria of the Burkholderia cepacia complex (Bcc). However, and despite the hundreds of sRNAs identified so far, the number of functionally characterized sRNAs from these bacteria remains very limited. In this mini-review, we describe the general characteristics of sRNAs and the main mechanisms involved in their action as regulators of post-transcriptional gene expression, as well as the work done so far in the identification and characterization of sRNAs from Bcc. The number of functionally characterized sRNAs from Bcc is expected to increase and to add new knowledge on the biology of these bacteria, leading to novel therapeutic approaches to tackle the infections caused by these opportunistic pathogens, particularly severe among cystic fibrosis patients.

Key points
•Hundreds of sRNAs have been identified in Burkholderia cepacia complex bacteria (Bcc).
•A few sRNAs have been functionally characterized in Bcc.
•Functionally characterized Bcc sRNAs play major roles in metabolism, biofilm formation, and virulence.
The effect of prednisolone and a short-term prednisolone discontinuation for the diagnostic accuracy of FDG-PET/CT in polymyalgia rheumatica—a prospective study of 101 patients
Authors: Nielsen Andreas Wiggers , Hansen Ib Tønder , Nielsen Berit Dalsgaard , Kjær Søren Geill , Blegvad-Nissen Jesper , Rewers Kate , Sørensen Christian Møller , Hauge Ellen-Margrethe , Gormsen Lars Christian , Keller Kresten Krarup 
Published: April 2024

Abstract
Purpose
2-[18F]Fluoro-2-deoxy-D-glucose (FDG)–positron emission tomography (PET)/computed tomography (CT) has been suggested as an imaging modality to diagnose polymyalgia rheumatica (PMR). However, the applicability of FDG-PET/CT remains unclear, especially following glucocorticoid administration. This study aimed to investigate the diagnostic accuracy of FDG-PET/CT before and during prednisolone treatment, as well as following short-term prednisolone discontinuation.

Methods
Treatment naïve suspected PMR patients were clinically diagnosed at baseline and subsequently had an FDG-PET/CT performed. Patients diagnosed with PMR were administered prednisolone following the first FDG-PET/CT and had a second FDG-PET/CT performed after 8 weeks of treatment. Subsequently, prednisolone was tapered with short-term discontinuation at week 9 followed by a third FDG-PET/CT at week 10. An FDG-PET/CT classification of PMR/non-PMR was applied, utilizing both the validated Leuven score and a dichotomous PMR score. The final diagnosis was based on clinical follow-up after 1 year.

Results
A total of 68 and 27 patients received a final clinical diagnosis of PMR or non-PMR. A baseline FDG-PET/CT classified the patients as having PMR with a sensitivity/specificity of 86%/63% (Leuven score) and 82%/70% (dichotomous score). Comparing the subgroup of non-PMR with inflammatory diseases to the PMR group demonstrated a specificity of 39%/54% (Leuven/dichotomous score). After 8 weeks of prednisolone treatment, the sensitivity of FDG-PET/CT decreased to 36%/41% (Leuven/dichotomous score), while a short-term prednisolone discontinuation increased the sensitivity to 66%/60%.

Conclusion
FDG-PET/CT has limited diagnostic accuracy for differentiating PMR from other inflammatory diseases. If FDG-PET/CT is intended for diagnostic purposes, prednisolone should be discontinued to enhance diagnostic accuracy.

Trial registration
ClinicalTrials.gov (NCT04519580). Registered 17th of August 2020.
The prognostic and potentially immunomodulatory role of cartilage oligomeric matrix protein in patients with gastric and esophageal adenocarcinoma
Authors: Papadakos Konstantinos S. , Gorji-Bahri Gilar , Gialeli Chrysostomi , Hedner Charlotta , Hagerling Catharina , Svensson Maria C. , Jeremiasen Martin , Borg David , Fristedt Richard , Jirström Karin , Blom Anna M. 
Published: April 2024

Abstract
Background
Cartilage oligomeric matrix protein (COMP) is a novel regulator of the tumor microenvironment. Studies in colon cancer and pancreatobiliary adenocarcinoma have revealed COMP expression to be associated with decreased infiltration of immune cells in the tumor microenvironment. Herein, the expression of COMP was investigated in gastric and esophageal adenocarcinoma with particular reference to its the relationship with the immune microenvironment.

Methods
COMP expression was evaluated in tissue microarrays representing primary tumors from 159 patients with chemo- and radiotherapy naïve esophageal and gastric adenocarcinoma and 67 matched samples of lymph node metastases using immunohistochemistry. Additionally, collagen fibers were stained with Sirius Red and evaluated with the FIJI macro TWOMBLI algorithm.

Results
The expression of COMP in cancer cells in the entire cohort was associated with shorter overall survival (OS) (p = 0.013) and recurrence-free survival (RFS) (p = 0.029), while COMP expression in the stroma was correlated with shorter RFS (p = 0.042). Similar correlations were found for patients with gastric adenocarcinoma, whereas COMP expression was not prognostic in esophageal adenocarcinoma. Further, in the entire cohort, the expression of COMP in the stroma was correlated with exclusion of different populations of immune cells (CD8+, CD3+, FoxP3+, CD20+) from the tumor microenvironment. Finally, higher density and alignment of collagen fibers were correlated with the expression of COMP in the stroma.

Conclusions
Expression of COMP in gastric and esophageal adenocarcinoma was correlated with shorter OS and RFS. A reduced number of immune cells infiltrated the tumor microenvironment when COMP expression was detected. This phenomenon could be attributed to the denser collagen deposits, a hallmark of tumor fibrosis observed in COMP-expressing tumors.
Rethinking the sub-Antarctic terrestrial N-cycle: evidence for organic N acquisition by Marion Island grasses
Authors: Pallett Nita C. M. , Ripley Brad S. , Greve Michelle , Cramer Michael D. 
Published: April 2024

AbstractOrganic N (oN, e.g., amino acids) is an important N-resource for plants in soils replete with oN but not inorganic N (iN; i.e., NH4+ and NO3−), such as cold ecosystems with temperature-limited soil decomposition rates. However, sub-Antarctic literature assumes that plants only acquire iN, potentially underestimating plant-available N. We hypothesised that Marion Island (− 46.90°, 37.75°) grasses (Polypogon magellanicus, Poa cookii, Agrostis stolonifera and Poa annua) acquire oN and that oN relative to iN provision affects plant growth. We investigated oN and iN uptake and growth responses in two hydroponics experiments. In situ N (15N-glycine, 15NO3− and 15NH4+) acquisition was investigated at three field sites with decreasing faunal influence, thus iN input and microbial activity. When plants grown in mire water were supplied with 15N-glycine or 15NO3−, root δ15N enrichment was highest for glycine-supplied plants. In the second hydroponics experiment, plant N-uptake rates (nmol g biomass−1 s−1) were significantly higher for glycine than NO3−, but relative growth rates (g g−1 d−1) lower on glycine. There were species-specific biomass allocation responses to N concentration (4 mM and 0.4 mM) and N-form (glycine and NO3−). Glycine-supplied grasses at the low iN concentration field sites had significantly higher δ15N enrichment relative to those at sites with high iN, suggesting higher oN uptake when iN is limiting. We demonstrate the importance of accounting for oN acquisition in the sub-Antarctic. As a system with high soil oN relative to iN, plants may predominantly meet N-demands through oN rather than iN acquisition.
Occipital nerve stimulation in pediatric patients with refractory occipital neuralgia
Authors: Mossner James , Saleh Nour B. , Shahin Maryam N. , Rosenow Joshua M. , Raskin Jeffrey S. 
Published: April 2024

Abstract
Purpose
Occipital neuralgia (ON) is a disabling problem within the pediatric population. Many of these patients fail medical therapies and continue to suffer without further surgical management. Occipital nerve stimulation (ONS) is used to treat ON in the adult population leading to a 72–89% reduction in pain; however, there are limited studies regarding its use in the pediatric population. In this study, we examined the outcomes of ONS in pediatric patients with medically refractory ON.

Methods
We performed a chart review of pediatric patients at our institution who have undergone ONS for the same indications.

Results
We identified 3 patients at our institution who underwent ONS trial and/or permanent implantation for ON. One patient had complete pain relief after the trial and declined permanent implantation. The other patient had fewer attacks compared to his pre-trial baseline and controlled them by adjusting his permanent implant stimulation settings. The last patient had near complete relief of her symptoms and no longer required any pain medication.

Conclusion
Our study highlights the paucity of studies evaluating the utility of ONS in the pediatric ON population. Limited data from both the literature and our institution’s experience reveal that pediatric patients may benefit from trial and/or permanent implantation of ONS for medically refractory ON pain.
Impact of pulmonary hypertension on outcomes after TEER in patients suffering from mitral regurgitation
Authors: Jaeger Philippa , Toskas Ioannis , Henes Jessica-Kristin , Shcherbyna Serhii , Schwarz Frederic , Euper Miriam , Seizer Peter , Langer Harald , May Andreas E. , Geisler Tobias , Gawaz Meinrad , Schreieck Jürgen , Rath Dominik 
Published: April 2024

Abstract

Aim
Data on associations of invasively determined hemodynamic parameters with procedural success and outcomes in patients suffering from mitral regurgitation (MR) undergoing transcatheter edge-to-edge repair of the mitral valve (M-TEER) is limited.

Methods and results
We enrolled 239 patients with symptomatic MR of grade 2 + , who received M-TEER. All patients underwent extensive pre-interventional invasive hemodynamic measurements via right heart catheterization (mean pulmonary arterial pressure (mPAP), systolic- (PAPsys) and diastolic pulmonary arterial pressure (PAPdia), pulmonary arterial wedge pressure (PAWP), a-wave, v-wave, pulmonary vascular resistance (PVR), transpulmonary pressure gradient (TPG), cardiac index (CI), stroke volume index (SVI)). mPAP and PAWP at baseline were neither associated with procedural success, immediate reduction of MR, nor residual MR after 6 months of follow-up. The composite outcome (All-cause mortality (ACM) and/or heart failure induced rehospitalization (HFH)) and HFH differed significantly after M-TEER when stratified according to mPAP, PAWP, PAPdia, a-wave and v-wave. ACM was not associated with the afore mentioned parameters. Neither PVR, TPG, CI nor SVI were associated with the composite outcome and HFH, respectively. In multivariable analyses, PAWP was independently associated with the composite outcome and HFH. PVR and SVI were not associated with outcomes.

Conclusion
PAWP at baseline was significantly and independently associated with HFH and might serve as a valuable parameter for identifying patients at high risk for HFH after M-TEER. ACM and procedural success were not affected by pulmonary arterial pressure before M-TEER. We suggest that the post-capillary component of PH serves as the driving force behind the risk of HFH.
Fibrous dysplasia of the head and neck in Southern Finland: a retrospective study on clinical characteristics, diagnostics, and treatment
Authors: Vilos Isabella , Nieminen Mikko T. , Mäkitie Riikka E. 
Published: April 2024

Abstract
Purpose
Fibrous dysplasia (FD) is a rare genetic disease with benign bone tumors. FD can affect one (monostotic FD) or multiple bones (polyostotic FD), with craniofacial lesions being common. Because of its rarity, there are only few clinical reports on FD in the head and neck region and its clinical characteristics remain incompletely defined. This study aimed to determine patient demographics, symptoms, diagnostics, and given treatment in patients with FD of the head and neck in a Finnish population.

Methods
A retrospective review on all patients diagnosed with or treated for FD of the head and neck at the Helsinki University Hospital during 2005–2020.

Results
In total 74 patients were identified; 54% were male and the mean age 45 years. Overall 95% had monostotic FD. Mandibula and maxilla were the most common anatomic sites. Majority of patients had symptoms, most commonly pain and lesion growth, and 49% had extra-skeletal symptoms. For all, diagnosis was primarily based on imaging findings, biopsies were obtained from 41%. Altogether 54 patients (73%) were managed by observation only, 20 patients (27%) received treatment; ten bisphosphonates, six surgery and four both.

Conclusion
Although highly variable in its clinical manifestations, head and neck FD lesions are often symptomatic and impose risk for extra-skeletal complications. Treatment is often conservative but should be individually tailored. Future studies are encouraged to better define the disease characteristics and hopefully offer new treatment possibilities.
Cancer survivorship in urban people living with cancer following primary treatment: A secondary analysis of qualitative interview data
Authors: Mahmood Saood , Graham Florence , Cooke Samuel , Kane Ros , Nelson David 
Published: April 2024

Abstract
Purpose
Urban cancer survivors have been shown to have better opportunities for recovery of health and wellbeing than their rural counterparts. Whilst there is a considerable body of evidence that explores urban people with cancers’ experiences and outcomes, there is a dearth of research that explicitly explores ‘urban cancer survivorship’ in its own right. This study aimed to explore cancer survivorship in urban people living with cancer who have completed primary treatment.

Methods
Secondary analysis of in-depth interview data (n = 18) with adults living with cancer who resided in urban parts of the UK. Data were drawn from a broader study on self-management of people living with cancer. An adapted version of Foster and Fenlon’s recovery of health and wellbeing in cancer survivorship framework was used to inform the analysis of the data.

Results
Recovery of health and wellbeing was impacted by a variety of contributory factors, which had a largely positive impact. Access to amenities, social support, travel, and healthcare factors were opportunities for urban cancer survivors, whilst pollution, traffic and a lack of green spaces acted as challenges for health management.

Conclusion
This study demonstrated how urban residency acted as both a barrier and a facilitator to recovery of health and wellbeing in urban cancer survivors following the completion of primary treatment. Area of residence should be taken into account by health providers and policymakers supporting cancer survivorship and the views of those with lived experiences should be included in informing future practice.
Evaluation of the association between excessive screen use, sleep patterns and behavioral and cognitive aspects in preschool population. A systematic review
Authors: Merín Llanos , Toledano-González Abel , Fernández-Aguilar Luz , Nieto Marta , del Olmo Nuria , Latorre José M. 
Published: April 2024

AbstractIn this review, we analyzed the possible relationship between the excessive use of screens and sleep patterns, and how this may affect certain behavioral and cognitive factors in preschool children. The selection, extraction and synthesis of the data were conducted according to PRISMA guidelines. The search was carried out in the electronic databases Medline (PubMed), PsycINFO (American Psychological Association), Scopus and Web of Science (WOS). Of the 597 articles initially identified, 13 met the inclusion criteria. The risk of bias of the articles selected was evaluated using a specific scale created for this purpose. The results found indicate that excessive use of screens is associated with a negative impact on the duration and quality of sleep-in preschoolers, and this worsening of sleep in infancy is related with a greater probability of the appearance of internalizing and externalizing behavioral problems and certain cognitive problems. The results also suggest that sleep could play a mediating or moderating role as a bioregulatory system that attenuates or increases the onset of behavioral and cognitive difficulties in those children most exposed to digital devices.
Analytical and experimental studies on toppling behavior of blocks misaligned with the slope face
Authors: Gui Jing-Yun , Alejano Leandro R. , Cano Miguel , Arzúa Javier , Pérez-Rey Ignacio 
Published: April 2024

AbstractThe stability of potentially toppling rock blocks has been typically addressed for the case of regular block geometries showing symmetry planes and resting on surfaces aligned with the slope face, i.e., the strike of the slope is parallel to the block sides and their basal planes. However, these simple geometries with ideally oriented blocks are not often found in nature. This study aims to bridge this knowledge gap through analytical and experimental approaches, in the form of laboratory physical models, to study toppling cases for non-conventional scenarios. An engineering geology review was first conducted, identifying potential non-standard cases in nature. Then, the current analytical solutions for both the factor of safety and critical toppling angle were modified for these misaligned cases, focusing on single block and standard block toppling, and considering the effect of block-edge rounding. Physical models support the analytical approach. It is concluded that toppling stability is slightly improved when the orientation of the basal plane of the blocks differs with the strike of the slope. In this way, for single blocks, the critical toppling angle increases a few tenths of a degree when the difference between strikes is 10°, about 1° with strike differences about 20° and 2 to 3° for misalignments about 30°. Similar increases are observed for block toppling cases. Accordingly, small differences in strike of basal planes and the slope contribute to slightly increasing the factor of safety of slopes prone to block toppling. This approach improves practical engineering toppling stability analyses.