bluuebunny/crossref_metadata_2025_split huggingface dataset:
Affordable Method for Hematocrit Determination in Murine Models
Authors: Branfield Siobhan , Washington A. Valance , Manfredi Barbara 
Published: April 2024

AbstractHematocrit (Hct) is a powerful tool often used in a clinical setting for the diagnosis of blood conditions such as anemia. It is also used in the research field as a hematological parameter in both human and mouse models. Measuring Hct, however, involves the use of expensive standardized equipment (such as a CritSpin™ Microhematocrit Centrifuge). Here, we describe a novel, simple, and affordable method to determine the Hct in untreated wild‐type (WT) mice and phenylhydrazine (PHZ)‐induced anemic mice with reasonable accuracy, using a benchtop centrifuge commonly available in laboratories. Hct of murine samples processed with a benchtop centrifuge, when compared to the standardized method CritSpin™, showed comparable results. This approach for determining Hct of murine can prove useful to research laboratories that cannot afford specialized equipment for Hct studies. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Affordable Method for Hematocrit Determination in Murine ModelsBasic Protocol 2: Murine Sample ValidationSupport Protocol: Phenylhydrazine‐induced anemia in wild‐type (WT) mice
On the Chemical Pathways Influencing the Effective Global Warming Potential of Commercial Hydrofluoroolefin Gases
Authors: Salierno Gabriel 
Published: April 2024

AbstractThe enforcement of a global hydrofluorocarbon (HFC) refrigerant phase down led to the introduction of hydrofluoroolefins (HFOs) as a low Global Warming Potential (GWP) substitute, given their low atmospheric lifetime. However, to this date it is not fully clear the long‐term atmospheric fate of HFOs primary degradation products: trifluoro acetaldehyde (TFE), trifluoro acetyl fluoride (TFF), and trifluoroacetic acid (TFA). It particularly concerns the possibility of forming HFC‐23, a potent global warming agent. Although the atmospheric reaction networks of TFE, TFF, and TFA have a fair level of complexity, the relevant atmospheric chemical pathways are well characterized in the literature, enabling a comprehensive hazard assessment of HFC‐23 formation as a secondary HFO breakdown product in diverse scenarios. A lower bound of the HFOs effective GWP in a baseline scenario is found above regulatory thresholds. While further research is crucial to refine climate risk assessments, the existing evidence suggests a non‐negligible climate hazard associated with HFOs.
Improved solar photovoltaic performance in standalone low‐voltage direct current microgrids using sensor fault tolerant control
Authors: Satya Sai Chandra M. V. , Mohapatro Sankarsan 
Published: April 2024

AbstractThe advancement of renewable energy technology has been significantly aided by solar photovoltaics (PV). Since solar PV is a weather‐dependent source, it cannot be dispatched. To ensure that the solar PV system can harvest the maximum amount of electricity for the available irradiance level, maximum power point tracking (MPPT) algorithms are used. For standalone low‐voltage DC (LVDC) microgrids to utilize the energy storage system as efficiently as possible, maximum power extraction is essential. The sensed PV voltage and current are essential for these MPPT algorithms to ensure that the maximum power point of the panel is captured. This work proposes an effective fault‐tolerant control (FTC) scheme for the solar PV subsystem in the LVDC microgrid that can seamlessly extract the maximum power despite the PV voltage sensor being faulty. The proposed FTC scheme uses a sliding mode observer (SMO)‐based method to detect and isolate PV voltage sensor faults in the standalone LVDC microgrid. The efficacy of the proposed FTC is assessed in a range of circumstances involving load disturbance, irradiance change, and various sensor fault scenarios. The performance of the proposed FTC is validated using experimental analysis on the LVDC microgrid testbed and MATLAB simulations. Given a faulty PV voltage sensor, at a given operating condition of the microgrid, the proposed FTC scheme is successful in reducing the additional power burden on the battery storage by at least two times. Consequently, the additional discharge in terms of SoC is also seen to be decreased by at least 9%. The proposed FTC technique outperforms the popular MPPT approaches for solar PV in terms of PV voltage sensor fault tolerance in the microgrid.
ALSTNet: Autoencoder fused long‐ and short‐term time‐series network for the prediction of tunnel structure
Authors: Du Bowen , Liang Haohan , Wang Yuhang , Ye Junchen , Tan Xuyan , Chen Weizhong 
Published: April 2024

AbstractIt is crucial to predict future mechanical behaviors for the prevention of structural disasters. Especially for underground construction, the structural mechanical behaviors are affected by multiple internal and external factors due to the complex conditions. Given that the existing models fail to take into account all the factors and accurate prediction of the multiple time series simultaneously is difficult using these models, this study proposed an improved prediction model through the autoencoder fused long‐ and short‐term time‐series network driven by the mass number of monitoring data. Then, the proposed model was formalized on multiple time series of strain monitoring data. Also, the discussion analysis with a classical baseline and an ablation experiment was conducted to verify the effectiveness of the prediction model. As the results indicate, the proposed model shows obvious superiority in predicting the future mechanical behaviors of structures. As a case study, the presented model was applied to the Nanjing Dinghuaimen tunnel to predict the stain variation on a different time scale in the future.
Evolution law of pulsating seepage and thermal deformation by injecting high‐temperature steam into coal for thermal coalbed methane recovery
Authors: Li Zhiqiang , Li Junliang , Chen Jinsheng , Karrech Ali , Zhang Ningchao , Chang Ju , Jin Kaiqi , Yu Yangyang , Wang Hongbin , Wang Aijie 
Published: April 2024

AbstractChinese coal reservoirs are characterized by low pressure and low permeability, which need to be enhanced so as to increase production. However, conventional methods for permeability enhancement can only increase the permeability in fractures, but not the ultra‐low permeability in coal matrices. Attempts to enhance such impermeable structures lead to rapid attenuation of gas production, especially in the late stage of gas extraction. Thermal stimulation by injecting high‐temperature steam is a promising method to increase gas production. The critical scientific challenges that still hinder its widespread application are related to the evolution law of permeability of high‐temperature steam in coal and the thermal deformation of coal. In this study, an experimental approach is developed to explore the high‐temperature steam seepage coupled with the thermal deformation in coal under triaxial stress. The tests were conducted using cylindrical coal specimens of ϕ50 mm × 100 mm. The permeability and thermal strain in coal were investigated when high‐temperature steam was injected at 151.11, 183.20, 213.65, and 239.76°C. The experimental results reveal for the first time that as the amount of injected fluid increases, the steam permeability shows periodic pulsation changes. This paper introduces and explains the main traits of this discovery that may shed more light on the seepage phenomenon. When the injected steam temperature increases, the amplitude of pulsating permeability decreases, whereas the frequency increases; meanwhile, the period becomes shorter, the pulsation peak appears earlier, and the stabilization time becomes longer. The average peak permeability shows a “U‐shaped” trend, decreasing first and then increasing as the steam temperature increases. Meanwhile, with the extension of steam injection time, the axial, radial, and volumetric strains of coal show a stage‐wise expansion characteristic at different temperatures of steam injection, except for the radial strains at 151.11°C. A two‐phase flow theory of gas–liquid is adopted to elucidate the mechanism of pulsating seepage of steam. Moreover, the influencing mechanism of inward and outward thermal expansion on the permeability of coal is interpreted. The results presented in this paper provide new insight into the feasibility of thermal gas recovery by steam injection.
Variable parallelism in the genomic basis of age at maturity across spatial scales in Atlantic Salmon
Authors: Kess Tony , Lehnert Sarah J. , Bentzen Paul , Duffy Steven , Messmer Amber , Dempson J. Brian , Newport Jason , Whidden Christopher , Robertson Martha J. , Chaput Gerald , Breau Cindy , April Julien , Gillis Carole‐Anne , Kent Matthew , Nugent Cameron M. , Bradbury Ian R. 
Published: April 2024

AbstractComplex traits often exhibit complex underlying genetic architectures resulting from a combination of evolution from standing variation, hard and soft sweeps, and alleles of varying effect size. Increasingly, studies implicate both large‐effect loci and polygenic patterns underpinning adaptation, but the extent that common genetic architectures are utilized during repeated adaptation is not well understood. Sea age or age at maturation represents a significant life history trait in Atlantic Salmon (Salmo salar), the genetic basis of which has been studied extensively in European Atlantic populations, with repeated identification of large‐effect loci. However, the genetic basis of sea age within North American Atlantic Salmon populations remains unclear, as does the potential for a parallel trans‐Atlantic genomic basis to sea age. Here, we used a large single‐nucleotide polymorphism (SNP) array and low‐coverage whole‐genome resequencing to explore the genomic basis of sea age variation in North American Atlantic Salmon. We found significant associations at the gene and SNP level with a large‐effect locus (vgll3) previously identified in European populations, indicating genetic parallelism, but found that this pattern varied based on both sex and geographic region. We also identified nonrepeated sets of highly predictive loci associated with sea age among populations and sexes within North America, indicating polygenicity and low rates of genomic parallelism. Despite low genome‐wide parallelism, we uncovered a set of conserved molecular pathways associated with sea age that were consistently enriched among comparisons, including calcium signaling, MapK signaling, focal adhesion, and phosphatidylinositol signaling. Together, our results indicate parallelism of the molecular basis of sea age in North American Atlantic Salmon across large‐effect genes and molecular pathways despite population‐specific patterns of polygenicity. These findings reveal roles for both contingency and repeated adaptation at the molecular level in the evolution of life history variation.
Video evidence that cuckoos farm their hosts by ejecting nestlings
Authors: Zhang Jinggang , Santema Peter , Li Jianqiang , Deng Wenhong , Kempenaers Bart 
Published: April 2024

AbstractWhen host nests are scarce, avian brood parasites would benefit from behaviours that increase the availability of suitable nests. Several studies reported ejection of host nestlings from nests by brood parasites; however, whether brood parasites do so to induce the host to re‐nest and thus increase opportunities for future parasitism (i.e. ‘farming’ behaviour) remains unclear. Here, we report observational evidence of farming behaviour by a common cuckoo Cuculus canorus female in a Daurian redstart Phoenicurus auroreus population: (1) the cuckoo destroyed a host nest by ejecting all nestlings, (2) the host then produced a new nest and (3) the cuckoo successfully parasitized the replacement nest. We suggest that farming behaviour may be more common, but often goes undetected because it requires intense nest monitoring.
Genetic analysis of Octopus cyanea reveals high gene flow in the South‐West Indian Ocean
Authors: Treleven Charles R. , Kishe Mary A. , Silas Mathew O. , Ngatunga Benjamin P. , Kuboja Bigeyo N. , Mgeleka Said S. , Taylor Amy L. , Elsmore Megan A. M. , Healey Amy J. E. , Sauer Warwick H. H. , Shaw Paul W. , McKeown Niall J. 
Published: April 2024

AbstractOctopus cyanea (Gray, 1849), abundant in the South‐West Indian Ocean (SWIO), constitutes a vital resource for both subsistence and commercial fisheries. However, despite this socioeconomic importance, and recent indications of overfishing, little is known about the population structure of O. cyanea in the region. To inform sustainable management strategies, this study assessed the spatio‐temporal population structure and genetic variability of O. cyanea at 20 sites in the SWIO (Kenya, Tanzania, Mozambique, Madagascar, Mauritius, Rodrigues, and the Seychelle Islands) by complementary analysis of mitochondrial DNA (mtDNA) noncoding region (NCR) sequences and microsatellite markers. MtDNA analysis revealed a shallow phylogeny across the region, with demographic tests suggesting historic population fluctuations that could be linked to glacial cycles. Contrary to expectations, NCR variation was comparable to other mtDNA regions, indicating that the NCR is not a hypervariable region. Both nuclear and mtDNA marker types revealed a lack of genetic structure compatible with high gene flow throughout the region. As adults are sedentary, this gene flow likely reflects connectivity by paralarval dispersal. All samples reported heterozygote deficits, which, given the overall absence of structure, likely reflect ephemeral larval recruitment variability. Levels of mtDNA and nuclear variability were similar at all locations and congruent with those previously reported for harvested Octopodidae, implying resilience to genetic erosion by drift, providing current stock sizes are maintained. However, as O. cyanea stocks in the SWIO represent a single, highly connected population, fisheries may benefit from additional management measures, such as rotational closures aligned with paralarval ecology and spanning geopolitical boundaries.
Corydalis sunhangii (Papaveraceae): A new species from Xizang, China, based on plastome and morphological data
Authors: Chen Jun‐Tong , Lidén Magnus , Huang Xian‐Han , Yang Shun‐Quan , Zhang Xin‐Jian , Liu Qun , Su Qi‐Lun , Hua Guo‐Jun , Luo Jian , Deng Tao 
Published: April 2024

AbstractA new species of Papaveraceae, Corydalis sunhangii, in the section Trachycarpae, is described and illustrated from Nyingchi City, Xizang, China. The new species has some resemblance to Corydalis kingdonis, but differs by radical leaves prominent, usually several, blade tripinnate (vs. insignificant, few, blade bi‐ to triternate); cauline leaf usually one, much smaller than radical leaves, usually situated in lower half of stem (vs. usually two, larger than radical leaves, concentrated in upper third of stem); racemes densely 13–35‐flowered (vs. rather lax, 4–11‐flowered); claw of lower petal shallowly saccate (vs. very prominently and deeply saccate); capsule oblong, with raised lines of dense papillae (vs. broadly obovoid, smooth). Phylogenetic analysis, based on 68 protein‐coding plastid genes of 49 samples, shows that C. sunhangii is not closely related to any hitherto described species, which is consistent with our morphological analysis.
The efficacy of the National Surgical Quality Improvement Program surgical risk calculator in head and neck surgery: A meta‐analysis
Authors: Harris Jacob , Ahluwalia Vinayak , Xu Katherine , Romeo Dominic , Fritz Christian , Rajasekaran Karthik 
Published: April 2024

AbstractBackgroundThe National Surgical Quality Improvement Program surgical risk calculator (SRC) estimates the risk for postoperative complications. This meta‐analysis assesses the efficacy of the SRC in the field of head and neck surgery.MethodsA systematic review identified studies comparing the SRC's predictions to observed outcomes following head and neck surgeries. Predictive accuracy was assessed using receiver operating characteristic curves (AUCs) and Brier scoring.ResultsNine studies totaling 1774 patients were included. The SRC underpredicted the risk of all outcomes (including any complication [observed (ob) = 35.9%, predicted (pr) = 21.8%] and serious complication [ob = 28.7%, pr = 17.0%]) except mortality (ob = 0.37%, pr = 1.55%). The observed length of stay was more than twice the predicted length (p < 0.02). Discrimination was acceptable for postoperative pneumonia (AUC = 0.778) and urinary tract infection (AUC = 0.782) only. Predictive accuracy was low for all outcomes (Brier scores ≥0.01) and comparable for patients with and without free‐flap reconstructions.ConclusionThe SRC is an ineffective instrument for predicting outcomes in head and neck surgery.
Differences in survival and recurrence of colorectal cancer by stage across population‐based European registries
Authors: Bouvier Anne‐Marie , Jooste Valérie , Lillini Roberto , Marcos‐Gragera Rafael , Katalinic Alexander , Giorgi Rossi Paolo , Launoy Guy , Bouvier Véronique , Guevara Marcela , Ardanaz Eva , Rapiti Aylward Elisabetta , Innos Kaire , Barranco Miguel Rodríguez , Sant Milena ,   for the European High Resolution Working Group on Colorectum
Published: April 2024

AbstractRecurrence after colorectal cancer resection is rarely documented in the general population while a key clinical determinant for patient survival. We identified 8785 patients with colorectal cancer diagnosed between 2010 and 2013 and clinically followed up to 2020 in 15 cancer registries from seven European countries (Bulgaria, Switzerland, Germany, Estonia, France, Italy, and Spain). We estimated world age‐standardized net survival using a flexible cumulative excess hazard model. Recurrence rates were calculated for patients with initially resected stage I, II, or III cancer in six countries, using the actuarial survival method. The proportion of nonmetastatic resected colorectal cancers varied from 58.6% to 78.5% according to countries. The overall 5‐year net survival by country ranged between 60.8% and 74.5%. The absolute difference between the 5‐year survival extremes was 12.8 points for stage II (Bulgaria vs Switzerland), 19.7 points for stage III (Bulgaria vs. Switzerland) and 14.8 points for Stage IV and unresected cases (Bulgaria vs. Switzerland or France). Five‐year cumulative rate of recurrence among resected patients with stage I–III was 17.7%. As compared to the mean of the whole cohort, the risk of developing a recurrence did not differ between countries except a lower risk in Italy for both stage I/II and stage III cancers and a higher risk in Spain for stage III. Survival after colorectal cancer differed across the concerned European countries while there were slight differences in recurrence rates. Population‐based collection of cancer recurrence information is crucial to enhance efforts for evidence‐based management of colorectal cancer follow up.
Clinical efficacy of tumor organoid‐guided cancer therapy for locally advanced unresectable or metastatic breast cancer
Authors: Lin Ying‐yi , Gao Hong‐fei , Li Hong , Hu Qiong , Du Bo‐le , Li Sheng , Xu Fang‐ping , Cheng Min‐yi , Zou Jia‐chen , Zheng Xing‐xing , Zhu Teng , Wang Kun 
Published: April 2024

AbstractPatient‐derived organoids (PDOs) may facilitate treatment selection. This retrospective cohort study evaluated the feasibility and clinical benefit of using PDOs to guide personalized treatment in metastatic breast cancer (MBC). Patients diagnosed with MBC were recruited between January 2019 and August 2022. PDOs were established and the efficacy of customized drug panels was determined by measuring cell mortality after drug exposure. Patients receiving organoid‐guided treatment (OGT) were matched 1:2 by nearest neighbor propensity scores with patients receiving treatment of physician's choice (TPC). The primary outcome was progression‐free survival. Secondary outcomes included objective response rate and disease control rate. Targeted gene sequencing and pathway enrichment analysis were performed. Forty‐six PDOs (46 of 51, 90.2%) were generated from 45 MBC patients. PDO drug screening showed an accuracy of 78.4% (95% CI 64.9%–91.9%) in predicting clinical responses. Thirty‐six OGT patients were matched to 69 TPC patients. OGT was associated with prolonged median progression‐free survival (11.0 months vs. 5.0 months; hazard ratio 0.53 [95% CI 0.33–0.85]; p = .01) and improved disease control (88.9% vs. 63.8%; odd ratio 4.26 [1.44–18.62]) compared with TPC. The objective response rate of both groups was similar. Pathway enrichment analysis in hormone receptor‐positive, human epidermal growth factor receptor 2‐negative patients demonstrated differentially modulated pathways implicated in DNA repair and transcriptional regulation in those with reduced response to capecitabine/gemcitabine, and pathways associated with cell cycle regulation in those with reduced response to palbociclib. Our study shows that PDO‐based functional precision medicine is a feasible and effective strategy for MBC treatment optimization and customization.
Light exposure and its applications in human health
Authors: Li Angze , Wei Xiaoling , Xie Yajia , Ren Yi , Zhu Xi , Liu Muqing , Liu Shangfeng 
Published: April 2024

AbstractLight exposure has been proven to have a significant impact on human health. As a result, researchers are increasingly exploring its potential benefits and drawbacks. With advancements in understanding light and the manufacturing of light sources, modern health lighting has become widely utilized in daily life and plays a critical role in the prevention and treatment of various illnesses. The use of light in healthcare is a global trend, with many countries actively promoting the development and application of relevant scientific research and medical technology. This field has gained worldwide attention and support from scientists and doctors alike. In this review, we examine the application of lighting in human health and recent breakthroughs in light exposure related to pathology, therapeutic strategies, molecular changes, and more. Finally, we also discuss potential future developments and areas of application.
Climate change exacerbates the compounding of heat stress and flooding in the mid‐latitudes
Authors: Treppiedi Dario , Villarini Gabriele , Noto Leonardo V. 
Published: April 2024

AbstractHeat stress and flood impacts have been extensively studied separately because of their significant societal and economic impacts, albeit apart from each other. Here we show that heat stress can trigger floods across large areas of North and South America, southern Africa, Asia and eastern Australia. We also show that the compounding of heat stress and floods is projected to worsen under climate change. This effect is magnified as we move from the Shared Socioeconomic Pathways (SSPs) 1–2.6 to 5–8.5. Moreover, in the future, the compounding between heat stress and floods is projected to extend to Europe and Russia, two areas where it has not been identified as relevant in the past. Moreover, by intersecting our results with future projections of the population of urban agglomerations, we find that heat stress/flood compound can pose a serious risk to a large portion of the world population. These results highlight the need towards improved preparation and mitigation measures that account for the compound nature of heat stress and flooding, and how the compounding is expected to be exacerbated because of climate change.