bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional evidence implicating S100P in prostate cancer progression
Authors: Basu Gargi D. , Azorsa David O. , Kiefer Jeffrey A. , Rojas Angela M. , Tuzmen Sukru , Barrett Michael T. , Trent Jeffrey M. , Kallioniemi Olli , Mousses Spyro 
Published: May 2008

AbstractS100P protein regulates calcium signal transduction and mediates cytoskeletal interaction, protein phosphorylation and transcriptional control. We have previously shown how elevated S100P levels in prostate cancer strongly correlate with progression to metastatic disease. In our study, we evaluated the functional significance of S100P expression on prostate tumor growth in vitro and in vivo. S100P levels were modulated by overexpressing S100P in PC3 prostate cancer cells and by silencing S100P levels in 22Rv1 prostate cancer cells. Overexpression of S100P in PC3 cells promoted cell growth, increased the percentage of S‐phase cells, decreased basal apoptosis rate and promoted anchorage independent growth in soft agar. Furthermore, prostate cancer cells overexpressing S100P were protected against camptothecin‐induced apoptosis. Conversely, silencing of S100P in 22Rv1 cells using siRNA resulted in a prominent cytostatic effect. The influence of S100P on tumor growth and metastases were assessed in vivo. S100P‐overexpressing PC3 cells had a dramatically increased tumor formation compared to controls. Microarray analysis showed the involvement of growth pathways including increased androgen receptor expression in S100P‐overexpressing cells. These results provide the first functional proof that S100P overexpression can upregulate androgen receptor expression and thereby promote prostate cancer progression by increasing cell growth. Moreover, the results confirm the oncogenic nature of S100P in prostate cancer and suggest that the protein may directly confer resistance to chemotherapy. Hence, S100P could be considered a potential drug target or a chemosensitization target, and could also serve as a biomarker for aggressive, hormone‐refractory and metastatic prostate cancer. © 2008 Wiley‐Liss, Inc.
Recent trends in prostate cancer mortality show a continuous decrease in several countries
Authors: Bouchardy Christine , Fioretta Gerald , Rapiti Elisabetta , Verkooijen Helena Maria , Rapin Charles Henry , Schmidlin France , Miralbell Raymond , Zanetti Roberto 
Published: May 2008

AbstractProstate specific antigen (PSA) screening was introduced to detect prostate cancer at an early stage and to reduce prostate cancer‐specific mortality. Until results from clinical trials are available, the efficacy of PSA screening in reducing prostate cancer mortality can be estimated by surveillance of prostate cancer mortality trends. Our study analyzes recent trends in prostate cancer mortality in 38 countries. We used the IARC‐WHO cancer mortality database and performed joinpoint analysis to examine prostate cancer mortality trends and identified 3 patterns. In USA, and to a lesser extent in Germany, Switzerland, Canada, France, Italy and Spain, prostate cancer‐specific mortality decreased to a level lower than before the introduction of PSA screening. In Australia, New Zealand, Austria, Finland, The Netherlands, Norway, United Kingdom, Hungary, Slovakia, Israel, Singapore, Sweden and Portugal, mortality from prostate cancer decreased but rates remain higher than before the introduction of PSA screening. Prostate cancer mortality continued to increase in Belgium, Denmark, Greece, Ireland, Bulgaria, Czech Republic, Belarus, Ukraine, Russian Federation, Romania, Poland, Argentina, Chile, Cuba, Mexico, Japan, China Hong Kong and the Republic of Korea. The trends in prostate cancer mortality rates in examined countries suggest that PSA screening may be effective in reducing mortality from prostate cancer. © 2008 Wiley‐Liss, Inc.
Primary human keratinocytes externalize stratifin protein via exosomes
Authors: Chavez‐Muñoz Claudia , Morse Jennifer , Kilani Ruhangiz , Ghahary Aziz 
Published: May 2008

AbstractAlthough, stratifin (SFN) is externalized by keratinocytes and stimulates the expression of matrix metalloproteinase‐1 (MMP‐1) in fibroblasts, its mechanism of externalization is not known. Here, we hypothesize that keratinocytes have a capacity to release stratifin through externalization of exosomes. To test this hypothesis, exosomes were purified from human keratinocyte conditioned medium (KCM) and analyzed for the presence of SFN by Western blot analysis using lysosomal‐associated membrane protein 2 (LAMP‐2) and heat shock cognate 70 (hsc70) as exosomal markers. The results showed the presence of SFN in keratinocyte lysate, concentrated KCM and exosomes, but not in concentrated unconditioned medium. Transmission electron microscopic examination revealed the presence of unique “saucer‐like” structures characteristic of exosomes whose diameters were <100 nm. Similar to the recombinant SFN, the exosomes associated proteins stimulated MMP‐1 expression in fibroblasts. Depletion of the exosomes markedly reduced this MMP‐1 stimulatory effect. To further statistically confirm these findings, fibroblasts were treated with three different exosome preparations and the finding showed more than 7.4‐fold increase in the level of MMP‐1 in the treated cells. Furthermore, we found that approximately 1% of the total proteins contained in exosomes correspond to SFN. In conclusion, this study is the first report showing that keratinocytes have the capacity to produce exosomes through which some intracellular proteins such as SFN, with MMP‐1 stimulating activity for fibroblasts, is externalized into keratinocyte microenvironment. J. Cell. Biochem. 104: 2165–2173, 2008. © 2008 Wiley‐Liss, Inc.
Hydration of ionic species studied by the reference interaction site model with a repulsive bridge correction
Authors: Chuev Gennady N. , Fedorov Maxim V. , Chiodo Sandro , Russo Nino , Sicilia Emilia 
Published: May 2008

AbstractWe have tested the reference interaction site model (RISM) for the case of the hypernetted chain (HNC) and the partially linearized hypernetted chain (PLHNC) closures improved by a repulsive bridge correction (RBC) for ionic hydrated species. We have analyzed the efficiency of the RISM/HNC+RBC and RISM/PLHNC+RBC techniques for decomposition of the electrostatic and the nonpolar hydration energies on the energetic and the enthalpic parts for polyatomic ions when the repulsive bridge correction is treated as a thermodynamic perturbation, and investigate the repulsive bridge effect on the electrostatic potential induced by solvent on solute atoms. For a number of univalent and bivalent atomic ions, molecular cations, and anions, the method provides hydration energies deviating only by several percents from the experimental data. In most cases, the enthalpic contributions to the free energies are also close to the experimental results. The above models are able to satisfactory predict the hydration energies as well as the electrostatic potential around the ionic species. For univalent atomic ions, they also provide qualitative estimates of the Samoilov activation energies. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2008
A computational study of the crystal and electronic structure of the room temperature organometallic ferromagnet V(TCNE)2
Authors: Tchougréeff Anderi L. , Dronskowski Richard 
Published: May 2008

AbstractWe study numerically the crystal and electronic structure of the room temperature organometallic ferromagnet of general composition V(TCNE)x × y solvent with x ≈ 2, starting from both the experimental structure of its iron analog which results from the EXAFS experiment as well as the theoretical model structure compatible with magnetic measurements on this type of compounds. The results of the numerical study performed at the density functional level of theory show that the experimentally determined structure complies with the magnetic measurements and thus can serve as a prototype structure for the entire family of the M(TCNE)2 organometallic magnets. Both the results of the numerical study and the magnetic experiments are interpreted using a proposed model Hamiltonian. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2008
Insights into cellular microRNAs and human immunodeficiency virus type 1 (HIV‐1)
Authors: Kumar Ajit , Jeang Kuan‐Teh 
Published: May 2008

AbstractRecent findings suggest that mammalian microRNAs (miRNAs) may influence viral replication in host cells. Studies on HIV‐1 infection have contributed in part to the development of this notion. Herein, we review, in brief, some of the evidence supportive of an interplay between human miRNAs and HIV‐1 in cells. Several cellular miRNAs potentially act to restrict HIV‐1 replication, and the virus has countermeasures to evade such restriction. J. Cell. Physiol. 216: 327–331, 2008. © 2008 Wiley‐Liss, Inc.
Comparable morphometric data of pathological nerve obtained using the three‐window sampling method and total fiber quantification
Authors: Agthong Sithiporn , Chentanez Vilai , Koonam Junjira , Kaewsema Atitaya 
Published: May 2008

AbstractMorphometric analysis of nerve biopsy provides data of structural changes that are essential for early detection of peripheral neuropathy. Because of the laborious work associated with the total fiber quantification, various sampling methods have been introduced with controversial accuracy. Three‐window sampling technique has been recently proposed to provide the accurate morphometric data of normal human sural nerve. However, its application in the diseased nerve has not been validated. This study, therefore, compared the morphometric data of nerve biopsies from 12 patients with various neuropathies obtained using this sampling method and those obtained by total fiber analysis. Total number, density, and diameter of myelinated fibers including myelin thickness and g ratio were analyzed. Intraclass correlation coefficients ranging from 0.95 to 0.99 indicate the high agreement between the data derived from the two methods in these parameters. This finding suggests the accuracy of the three‐window sampling technique in the morphometric study of nerve with pathological alterations. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.
Odd‐angulated graphs and cancelling factors in box products
Authors: Che Zhongyuan , Collins Karen L. , Tardif Claude 
Published: May 2008

AbstractUnder what conditions is it true that if there is a graph homomorphism G □ H → G □ T, then there is a graph homomorphism H→ T? Let G be a connected graph of odd girth 2k + 1. We say that G is (2k + 1)‐angulated if every two vertices of G are joined by a path each of whose edges lies on some (2k + 1)‐cycle. We call G strongly (2k + 1)‐angulated if every two vertices are connected by a sequence of (2k + 1)‐cycles with consecutive cycles sharing at least one edge. We prove that if G is strongly (2k + 1)‐angulated, H is any graph, S, T are graphs with odd girth at least 2k + 1, and ϕ: G□ H→S□T is a graph homomorphism, then either ϕ maps G□{h} to S□{th} for all h∈V(H) where th∈V(T) depends on h; or ϕ maps G□{h} to {sh}□ T for all h∈V(H) where sh∈V(S) depends on h. This theorem allows us to prove several sufficient conditions for a cancelation law of a graph homomorphism between two box products with a common factor. We conclude the article with some open questions. © 2008 Wiley Periodicals, Inc. J Graph Theory 58:221‐238, 2008
Appling the abdominal aortic‐balloon occluding combine with blood pressure sensor of dorsal artery of foot to control bleeding during the pelvic and sacrum tumors surgery
Authors: Yang Liu , Chong‐qi Tu , Hai‐bo Song , Lan Zhang , Tian‐fu Yang , Hong Duan , Fu‐xing Pei 
Published: May 2008

AbstractBackground and ObjectivesTo investigate the feasibilities of reducing intraoperative hemorrhage and improving the safety of pelvic and sacrum tumor surgery using sizing balloon occluding abdominal aorta.MethodFrom May 2001 to May 2007, 18 patients were diagnosed as sacrum or pelvic tumor and underwent surgery in our institution. Balloon catheters were placed via femoral artery to occlude the abdominal aorta of pelvic tumor and sacrum region undergoing the sacrum resection or half pelvis resection and replacement operation in 12 patients. A sizing balloon was used to occlude the abdominal aorta for 60 min in assisting with resection of pelvic and sacral tumors.ResultsAfter the abdominal aorta was occluded, much less intraoperative hemorrhage was found, and the average blood loss was only 280 ml (range 200–600 ml). This procedure assisted the surgeon in identifying clearly the surgical margin and neurovascular structure surrounded by the tumors. The blood pressure remained stable during the operation. And the function of the kidney, the pelvis organs and the lower extremities were normal.ConclusionIntraoperative abdominal aorta occluding may effectively control intraoperative hemorrhage, thus assisting the surgeon in the complete and safe resection of pelvic and sacrum tumors. J. Surg. Oncol. 2008;97:626–628. © 2008 Wiley‐Liss, Inc.
Sociodemographic and tumor characteristics associated with pancreatic cancer surgery in the United States
Authors: Gawron Andrew J. , Gapstur Susan M. , Fought Angela J. , Talamonti Mark S. , Skinner Halcyon G. 
Published: May 2008

AbstractBackground and ObjectivesSurgical resection is the only curative treatment for pancreatic cancer. The aim of this project was to compare risk and prognostic factors between pancreatic cancer patients treated with and without surgery.MethodsData from 32,318 cases collected from 1988 to 2002 in the Surveillance Epidemiology and End Results program were used to evaluate the odds of having surgery for pancreatic adenocarcinoma. Multivariate logistic regression was used to estimate odds ratios and 95% confidence intervals for the associations of demographic and tumor characteristics with surgical treatment.ResultsOverall, men were less likely to have surgery than women (OR = 0.90, 95% CI 0.83, 0.98). Compared to married patients, surgery was significantly less likely among those who were divorced/separated (OR = 0.78, 95% CI 0.68, 0.89) or widowed (OR = 0.76, 95% CI 0.67, 0.85). Black patients were less likely to receive surgery than white patients (OR = 0.79, 95% CI 0.70, 0.91). Patients diagnosed in 1997–2002 were more likely to have surgery then those diagnosed in 1988–1996. Geographic differences also exist in the frequency of surgical treatment reported by different registries.ConclusionsThere are differences in sociodemographic characteristics between surgically and non‐surgically treated pancreatic cancers that are not entirely attributable to age, primary site, or stage at diagnosis. J. Surg. Oncol. 2008;97:578–582. © 2008 Wiley‐Liss, Inc.
Surgical intervention following imatinib treatment in patients with advanced gastrointestinal stromal tumors (GISTs)
Authors: Sym Sun Jin , Ryu Min‐Hee , Lee Jae‐Lyun , Chang Heung Moon , Kim Tae‐Won , Kim Hee Cheol , Kim Ki Hun , Yook Jeong Hwan , Kim Byung Sik , Kang Yoon‐Koo 
Published: May 2008

AbstractBackgroundWe investigated the role of surgical intervention for advanced GIST after imatinib.MethodsAmong 256 patients treated with imatinib for advanced GIST, the medical records of the 34 patients who underwent surgery of residual tumors after imatinib treatment were reviewed.ResultsSurgery was performed on 24 patients with responsive disease (RD) after imatinib, on 3 with focal progressive disease (FP), and on 7 with generalized progressive disease (GP). All gross tumors were completely resected in 19/24 (79%), 1/3 (33%), and 1/7 (14%) patients, respectively. Disease status at surgery was associated with prognosis after surgery; with a median follow‐up of 25.7 months, the median progression‐free survival of patients resected for RD, FP, and GP were 27.8 months (95% CI, 17.8–37.8 months), 5.1 months (95% CI, 4.7–5.6 months), and 3.3 months (95% CI, 2.7–3.9 months), respectively (P < 0.001). Median overall survival was not reached in patients resected for RD, and was 22.5 months (95% CI, 1.4–43.0 months) and 23.5 months (95% CI, 3.0–43.9) for patients resected for FP and GP, respectively (P < 0.001).ConclusionSurgical resection of tumors responsive to imatinib may be beneficial in patients with advanced GIST. Debulking surgery, however, is not recommended for patients who have already developed imatinib resistance. J. Surg. Oncol. 2008;98:27–33. © 2008 Wiley‐Liss, Inc.
Concentrator multijunction solar cell characteristics under variable intensity and temperature
Authors: Kinsey Geoffrey S. , Hebert Peter , Barbour Kent E. , Krut Dmitri D. , Cotal Hector L. , Sherif Raed A. 
Published: May 2008

AbstractThe performance of multijunction solar cells has been measured over a range of temperatures and illumination intensities. Temperature coefficients have been extracted for three‐junction cell designs that are in production and under development. A simple diode model is applied to the three‐junction performance as a means to predict performance under operating conditions outside the test range. These data may be useful in guiding the future optimization of concentrator solar cells and systems. Copyright © 2008 John Wiley & Sons, Ltd.
Highly accurate method for ligand‐binding site prediction in unbound state (apo) protein structures
Authors: Morita Mizuki , Nakamura Shugo , Shimizu Kentaro 
Published: May 2008

AbstractThis article describes a new method for predicting ligand‐binding sites of proteins. The method involves calculating the van der Waals interaction energy between a protein and probes placed on the protein surface, and then clustering the probes with attractive interaction to find the energetically most favorable locus. In 80% (28/35) of the test cases, the ligand‐binding site was successfully predicted on a ligand‐bound protein structure, and in 77% (27/35) was successfully predicted on an unbound structure. Our method was used to successfully predict ligand‐binding sites unaffected by induced‐fit as long as its scales were not very large, and it contributed to a significant improvement in prediction with unbound state protein structures. This represents a significant advance over conventional methods in detecting ligand‐binding sites on uncharacterized proteins. Moreover, our method can predict ligand‐binding sites with a narrower locus than those achieved using conventional methods. Proteins 2008. © 2008 Wiley‐Liss, Inc.
Effect of lipid composition on buforin II structure and membrane entry
Authors: Fleming Eleanor , Maharaj Natalya P. , Chen Jessica L. , Nelson Rachel B. , Elmore Donald E. 
Published: May 2008

AbstractBuforin II is a 21‐amino acid polycationic antimicrobial peptide derived from a peptide originally isolated from the stomach tissue of the Asian toadBufo bufo gargarizans. It is hypothesized to target a wide range of bacteria by translocating into cells without membrane permeabilization and binding to nucleic acids. Previous research found that the structure and membrane interactions of buforin II are related to lipid composition. In this study, we used molecular dynamics (MD) simulations along with lipid vesicle experiments to gain insight into how buforin II interacts differently with phosphatidylcholine (PC), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE) lipids. Fluorescent spectroscopic measurements agreed with the previous assertion that buforin II does not interact with pure PC vesicles. Nonetheless, the reduced entry of the peptide into anionic PG membranes versus neutral PC membranes during simulations correlates with the experimentally observed reduction in BF2 translocation through pure PG membranes. Simulations showing membrane entry into PC also provide insight into how buforin II may initially penetrate cell membranes. Our MD simulations also allowed us to consider how neutral PE lipids affect the peptide differently than PC. In particular, the peptide had a more helical secondary structure in simulations with PE lipids. A change in structure was also apparent in circular dichroism measurements. PE also reduced membrane entry in simulations, which correlates with decreased translocation in the presence of PE observed in previous studies. Together, these results provide molecular‐level insight into how lipid composition can affect buforin II structure and function and will be useful in efforts to design peptides with desired antimicrobial and cell‐penetrating properties. Proteins 2008. © 2008 Wiley‐Liss, Inc.
Are autosomal sex-determining factors of the housefly (Musca domestica) spreading north?
Authors: KOZIELSKA MAGDALENA , FELDMEYER BARBARA , PEN IDO , WEISSING FRANZ J. , BEUKEBOOM LEO W. 
Published: May 2008

SummaryMultiple sex-determining factors have been found in natural populations of the housefly,Musca domestica. Their distribution seems to follow a geographical cline. The ‘standard’ system, with a male-determining factor,M, located on the Y chromosome, prevails at higher latitudes and altitudes. At lower latitudes and altitudesMfactors have also been found on any of the five autosomes. Such populations often also harbour a dominant autosomal factor,FD, which induces female development even in the presence of severalMfactors. AutosomalMfactors were first observed some 50 years ago. It has been hypothesized that following their initial appearance, they are spreading northwards, replacing the standard XY system, but this has never been systematically investigated. To scrutinize this hypothesis, we here compare the current distribution of autosomalMfactors in continental Europe, on a transect running from Germany to southern Italy, with the distribution reported 25 years ago. Additionally, we analysed the frequencies of theFDfactor, which has not been done before for European populations. In contrast to earlier predictions, we do not find a clear change in the distribution of sex-determining factors: as 25 years ago, only the standard XY system is present in the north, while autosomalMfactors and theFDfactor are prevalent in Italy. We discuss possible causes for this apparently stable polymorphism.
A proximal decomposition of inbreeding, coancestry and contributions
Authors: COLLEAU JEAN-JACQUES , SARGOLZAEI MEHDI 
Published: May 2008

SummaryWright's (1922) coalescence probabilistic method decomposes the average coancestry or the average inbreeding of the population of interest into contributions of ancestors who are directly responsible for coancestry. These ancestors are less numerous and younger than those given by Caballero & Toro's (2000) method. Then, Wright's method yields a more parsimonious representation of the origin of coancestry or inbreeding. Results of Wright's method, called proximal because ancestors are younger, can be obtained from the latter method using a simple matrix transformation. Detailed algorithms are presented. Both methods can be used for assessing the overall efficiency of a breeding scheme in balancing genetic gain and coancestry rate, yielding very similar results when generations are discrete.