bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental impact analysis of lithium iron phosphate batteries for energy storage in China
Authors: Lin Xin , Meng Wenchuan , Yu Ming , Yang Zaimin , Luo Qideng , Rao Zhi , Zhang Tiangang , Cao Yuwei 
Published: February 2024

The deployment of energy storage systems can play a role in peak and frequency regulation, solve the issue of limited flexibility in cleaner power systems in China, and ensure the stability and safety of the power grid. This paper presents a comprehensive environmental impact analysis of a lithium iron phosphate (LFP) battery system for the storage and delivery of 1 kW-hour of electricity. Quantities of copper, graphite, aluminum, lithium iron phosphate, and electricity consumption are set as uncertainty and sensitivity parameters with a variation of [90%, 110%]. The results show that global warming potential is 9.08E+01 kg CO2 eq. and fossil resource use is 1.21E+03 MJ, with uncertainty ranges of [8.54E+01, 9.23E+01] and [1.15E+03, 1.23E+03], respectively. Electricity consumption during the manufacture and installation process is the greatest contributor to climate change (CO2 eq. emissions), accounting for 39.71% and largely owing to non-renewable sources, followed by cathode materials at 27.85% and anode materials at 18.36%. The disposal and recycling process offers emission reductions but requires an additional 1.17% use of fossil resources. Sobol T indices for the quantity of electricity are the highest for acidification, climate change, fossil resource use, and ionizing radiation. By considering the pathway of China’s electricity mix from 2020 to 2050 under the Paris Agreement’s 2° target, the potential for environmental emission reduction in the system is evaluated. The results show that the greener electricity mix could lead to a 24.59% reduction in acidification impact, a 35.74% reduction in climate change impact, a 33.24% reduction in fossil resource use, and a 44.13% reduction in ionizing radiation impact. This study offers a comprehensive view of the environmental impact reductions associated with the lithium iron phosphate battery and its industry.
Associations of the circulating levels of cytokines with risk of systemic sclerosis: a bidirectional Mendelian randomized study
Authors: Jiang Zong , Yao Xiaoling , Lan Weiya , Tang Fang , Ma Wukai , Yao Xueming , Chen Changming , Cai Xin 
Published: February 2024

ObjectiveSystemic sclerosis(SSc) remains unclear, studies suggest that inflammation may be linked to its pathogenesis. Hence, we conducted a bidirectional Mendelian randomization (MR) analysis to evaluate the association between cytokine and growth factor cycling levels and the risk of SSc onset.MethodsIn our study, the instrumental variables(IVs) for circulating cytokines were sourced from the genome-wide association study (GWAS) dataset of 8293 Finnish individuals. The SSc data comprised 302 cases and 213145 controls, and was included in the GWAS dataset. We employed four methods for the MR analysis: MR Egger, Inverse variance weighted (IVW), Weighted medium, and Weighted Mode, with IVW being the primary analytical method. Sensitivity analyses were performed using heterogeneity testing, horizontal pleiotropy testing, and the Leave One Out (LOO) method. We also conducted a reverse MR analysis to determine any reverse causal relationship between SSc and circulating cytokines.ResultsAfter Bonferroni correction, MR analysis revealed that the Interleukin-5 (IL-5) cycle level was associated with a reduced risk of SSc [odds ratio (OR)=0.48,95% confidence interval (CI): 0.27-0.84, P=0.01]. It also indicated that the Stem cell growth factor beta (SCGF-β) cycling level might elevate the risk of SSc (OR = 1.36, 95% CI: 1.01-1.83, P = 0.04). However, the reverse MR analysis did not establish a causal relationship between SSc and circulating cytokine levels. Additionally, sensitivity analysis outcomes affirm the reliability of our results.ConclusionOur MR study suggests potential causal relationships between IL-5, SCGF-β, and the risk of SSc. Further research is essential to determine how IL-5 and SCGF-β influence the development of SSc.
TLR5-deficiency controls dendritic cell subset development in an autoimmune diabetes-susceptible model
Authors: Pearson James Alexander , Hu Youjia , Peng Jian , Wong F. Susan , Wen Li 
Published: February 2024

IntroductionThe incidence of the autoimmune disease, type 1 diabetes (T1D), has been increasing worldwide and recent studies have shown that the gut microbiota are associated with modulating susceptibility to T1D. Toll-like receptor 5 (TLR5) recognizes bacterial flagellin and is widely expressed on many cells, including dendritic cells (DCs), which are potent antigen-presenting cells (APCs). TLR5 modulates susceptibility to obesity and alters metabolism through gut microbiota; however, little is known about the role TLR5 plays in autoimmunity, especially in T1D.MethodsTo fill this knowledge gap, we generated a TLR5-deficient non-obese diabetic (NOD) mouse, an animal model of human T1D, for study.ResultsWe found that TLR5-deficiency led to a reduction in CD11c+ DC development in utero, prior to microbial colonization, which was maintained into adulthood. This was associated with a bias in the DC populations expressing CD103, with or without CD8α co-expression, and hyper-secretion of different cytokines, both in vitro (after stimulation) and directly ex vivo. We also found that TLR5-deficient DCs were able to promote polyclonal and islet antigen-specific CD4+ T cell proliferation and proinflammatory cytokine secretion. Interestingly, only older TLR5-deficient NOD mice had a greater risk of developing spontaneous T1D compared to wild-type mice.DiscussionIn summary, our data show that TLR5 modulates DC development and enhances cytokine secretion and diabetogenic CD4+ T cell responses. Further investigation into the role of TLR5 in DC development and autoimmune diabetes may give additional insights into the pathogenesis of Type 1 diabetes.
Serum high mobility group box 1 as a potential biomarker for the progression of kidney disease in patients with type 2 diabetes
Authors: Liu Tongtong , Zhao Hailing , Wang Ying , Qu Peng , Wang Yanmei , Wu Xiai , Zhao Tingting , Yang Liping , Mao Huimin , Peng Liang , Zhan Yongli , Li Ping 
Published: February 2024

BackgroundAs a damage-associated molecular pattern protein, high mobility group box 1 (HMGB1) is associated with kidney and systemic inflammation. The predictive and therapeutic value of HMGB1 as a biomarker has been confirmed in various diseases. However, its value in diabetic kidney disease (DKD) remains unclear. Therefore, this study aimed to investigate the correlation between serum and urine HMGB1 levels and DKD progression.MethodsWe recruited 196 patients with type 2 diabetes mellitus (T2DM), including 109 with DKD and 87 T2DM patients without DKD. Additionally, 60 healthy participants without T2DM were also recruited as controls. Serum and urine samples were collected for HMGB1 analysis. Simultaneously, tumor necrosis factor receptor superfamily member 1A (TNFR-1) in serum and kidney injury molecule (KIM-1) in urine samples were evaluated for comparison.ResultsSerum and urine HMGB1 levels were significantly higher in patients with DKD than in patients with T2DM and healthy controls. Additionally, serum HMGB1 levels significantly and positively correlated with serum TNFR-1 (R2 = 0.567, p&lt;0.001) and urine KIM-1 levels (R2 = 0.440, p&lt;0.001), and urine HMGB1 has a similar correlation. In the population with T2DM, the risk of DKD progression increased with an increase in serum HMGB1 levels. Multivariate logistic regression analysis showed that elevated serum HMGB1 level was an independent risk factor for renal function progression in patients with DKD, and regression analysis did not change in the model corrected for multiple variables. The restricted cubic spline depicted a nonlinear relationship between serum HMGB1 and renal function progression in patients with DKD (p-nonlinear=0.007, p&lt;0.001), and this positive effect remained consistent across subgroups.ConclusionSerum HMGB1 was significantly correlated with DKD and disease severity. When the HMGB1 level was ≥27 ng/ml, the risk of renal progression increased sharply, indicating that serum HMGB1 can be used as a potential biomarker for the diagnosis of DKD progression.
IL-17A inhibitors alleviate Psoriasis with concomitant restoration of intestinal/skin microbiota homeostasis and altered microbiota function
Authors: Zhao Huixia , Shang Lili , Zhang Yuting , Liang Zhaojun , Wang Nan , Zhang Qian , Gao Chong , Luo Jing 
Published: February 2024

BackgroundDisturbed gut microbiota and associated metabolic dysfunction exist in Psoriasis. Despite the growing use of interleukin-17 inhibitor (anti-IL17) therapy, the effect of anti-IL17 on gut/skin microbiota function is not fully understood in patients with Psoriasis.ObjectiveTherefore, we explored whether Psoriasis is associated with alterations in selected gut/skin microbiota in a study cohort, and a longitudinal cohort study to reveal the effects of IL-17A inhibitor treatment on gut microbiota in Psoriasis.MethodsIn a case-control study, 14 patients with Psoriasis and 10 age, sex and body mass index-matched Healthy Controls were recruited. Longitudinal mapping of the gut microbiome was performed using 16S rRNA gene sequencing. Mouse models were used to further study and validate the interrelationship between the skin microbiome and the gut microbiome in Psoriasis. PICRUST2 was applied to predict the function of the bacterial community.ResultsIn Psoriasis patients, gut microbiota dysbiosis was present with increased heterogeneity: decreased Bacteroidota and increased Firmicutes as well as Actinobacteriota predominating in Psoriasis. Escherichia-Shigella enrichment was associated with reduction in serum levels of total bile acid and markers in Apoptotic pathways. After IL-17A inhibitor treatment in Psoriasis patients, longitudinal studies observed a trend toward a normal distribution of the gut microbiome and modulation of apoptosis-related metabolic pathways. Results from a mouse model showed dysregulation of the skin microbiota in Psoriasis characterized by Staphylococcus colonization.ConclusionThe psoriatic gut/skin microbiota exhibits loss of community stability and pathogen enrichment. IL-17A inhibitors restore microbiota homeostasis and metabolic pathways, reduce pro-inflammatory cytokine expression, and alleviate symptoms in patients with Psoriasis.
Porcine alveolar macrophages host proteins interacting with African swine fever virus p72
Authors: Weng Zhijun , Zheng Xiaoyu , Liang Yifan , Chen Xiongnan , Peng Qin , Zhang Guihong , Gong Lang , Zheng Zezhong 
Published: February 2024

IntroductionAfrican swine fever virus (ASFV) is a highly contagious virus that spreads rapidly and has a mortality rate of up to 100% in domestic pigs, leading to significant economic losses in the pig industry. The major capsid protein p72 of ASFV plays a critical role in viral invasion and immune evasion.MethodsIn this study, we used yeast two-hybrid screening to identify host proteins interacting with p72 in porcine alveolar macrophages (PAMs) and verified these proteins using confocal microscopy and immunoprecipitation techniques.Results and DiscussionWe validated 13 proteins that interact with p72, including CD63, B2M, YTHDF2, FTH1, SHFL, CDK5RAP3, VIM, PELO, TIMP2, PHYH, C1QC, CMAS, and ERCC1. Enrichment analysis and protein-protein interaction network analysis of these interacting proteins revealed their involvement in virus attachment, invasion, replication, assembly, and immune regulation. These findings provide new insights into the function of p72 and valuable information for future research on the interaction between ASFV and host proteins.
Assessment of focused ultrasound stimulation to induce peripheral nerve activity and potential damage in vivo
Authors: Rodríguez-Meana Bruno , Santos-Nogueira Eva , Trujillo-Vázquez Sònia , Jakob Anette , Udina Esther , Fournelle Marc , Navarro Xavier 
Published: February 2024

IntroductionPeripheral neuroprostheses are aimed to restore loss of sensory and motor functions by interfacing axons in the peripheral nerves. Most common interfaces in neuroprostheses are electrodes that establish electrical connection with peripheral axons. However, some challenges arise related to long-term functionality, durability, and body response. Recently, focused ultrasound stimulation (FUS) has emerged as a non-invasive approach to modulate the nervous system. However, it is controversial whether FUS can induce axon depolarization.MethodsWe have assessed FUS applied in vivo to the rat peripheral nerve, with two objectives: first, to test whether FUS activates peripheral nerves under different stimulation conditions, and second, to evaluate if FUS inflicts damage to the nerve. FUS was delivered with three ultrasound transducers (Sonic Concept H115, H107, and H102) covering the largest set of parameters examined for FUS of peripheral nerves so far.ResultsWe did not obtain reliable evoked action potentials in either nerves or muscles, under any FUS condition applied, neither over the skin nor directly to the nerve exposed. Additional experiments ex vivo and in vivo on mice, confirmed this conclusion. When FUS stimulation was applied directly to the exposed sciatic nerve, neuromuscular function decreased significantly, and recovered one week later, except for FUS at 0.25 MHz. Histologically, degenerating nerve fibers were observed, with a tendency to be higher with the lower FUS frequency.DiscussionPast reports on the ability of ultrasound to stimulate the peripheral nerve are controversial. After testing a wide range of FUS conditions, we conclude that it is not a reliable and safe method for stimulating the peripheral nerve. Special consideration should be taken, especially when low-frequency FUS is applied, as it may lead to nerve damage.
Creating healthy eating and active environments in early learning settings: protocol of the CHEERS eHealth intervention study
Authors: Lafave Lynne M. Z. , Hayek Joyce , Webster Alexis D. , McConnell Ceilidh 
Published: February 2024

BackgroundEarly childhood educators through their daily interactions with children, play a central role in shaping young children’s health behaviors. Given their influential role, early childhood educators are often targeted in interventions aiming at enhancing their nutrition and physical activity practices.MethodsThis paper presents the design of the CHEERS eHealth program to improve nutrition and physical activity practices within Early Childhood Education and Care (ECEC) centers. The study has a longitudinal quasi-experimental design with recruitment of ECECs across Alberta Canada. ECEC intervention group educators complete 12 weekly online nutrition and physical activity modules and participate in weekly communities of practice sessions to discuss practical applications within their centers. Outcome assessments are scheduled at baseline (T1), mid-point at 5 months (T2), and end of program after 10 months (T3). Outcome measures include the Creating Healthy Eating and Active Environments survey (CHEERS), Mindful Eating Questionnaire (MEQ), Canadian Behavior, Attitude and Nutrition Knowledge Survey (C-BANKS 2.0), Physical Literacy Knowledge, Attitude, Self-Efficacy, and Behavior (PLKASB-ECE), the Environment and Policy Assessment and Observation (EPAO) derived variables, and an objective measure of children’s physical activity using ActiGraph GT3X accelerometers. Linear mixed model analyses will be used to evaluate the effectiveness of the intervention. Qualitative assessments comprise exit interviews and open-response questions embedded within the educational modules.ResultsPreliminary baseline data from the 2019 cohort indicate no statistically significant differences between the intervention and control groups for the primary outcome variables, except age. Educators’ personal nutrition-related knowledge, attitude and behaviors were positively associated with their self-assessments of the nutrition environment and practices in ECECs. A significant correlation was observed between educators’ self-reported physical activity practices and observed activity practices. The CHEERS survey Food Served subscale showed a positive correlation with the objective measures of EPAO-Foods Provided and Nutrition Policy subdomains.DiscussionWe propose that this eHealth intervention would be an effective scaling up approach to enhancing the nutrition and physical activity environments of ECECs by fostering improved nutrition and physical activity-related knowledge, attitudes, and adherence to best practices which will potentially lead to improved outcomes for children in their care.
Effect of high NEFA concentration on lipid metabolism disorders in hepatocytes based on lipidomics
Authors: Fan Xinyi , Xu Jie , Hu Yuan , Wang Kui , Zhao Yiyi , Cai Jinyin , Zhang Xinyuan , Pan Binghai , Xu Anqi , Chen Yajing , Liu Songhao , Jiang Kangfeng , Li Xiaobing 
Published: February 2024

Introduction: High concentrations of nonesterified fatty acids (NEFA) is the key of characteristic of fatty liver in dairy cows. Therefore, the aim of this study was to investigate the effect of high concentration of NEFA on lipid metabolism in hepatocytes through the lipidomic approach and molecular biology techniques.Methods: Stimulate AML-12 cells with different concentrations of NEFA, observe the cellular lipid accumulation, and select 0.6 mM NEFA stimulation concentration for subsequent experiments. Collect cells for lipidomics analysis.Results: High concentration of NEFA (0.6–2.4 mM) significantly reduced the cell viability in a concentration-dependent manner, indicating that high concentrations of NEFA have lipotoxicity on hepatocytes. In addition, NEFA promoted triglycerides (TAG) accumulation, increased the mRNA expression of the lipogenic molecules SREBP1c and FASN, and decreased the mRNA expression of lipolytic molecules CPT1A and HSL in hepatocytes. Mechanistically, high concentration of NEFA induced lipid metabolism disorders in hepatocytes by regulating metabolic pathways such as glycerol phospholipid metabolism, glycosyl phosphatidylinositol anchored biosynthesis, triglyceride metabolism, sphingolipid metabolism, and inositol phosphate metabolism.Discussion: High concentration of NEFA is lipotoxic to cells, promoting lipid accumulation. LPE (18:2), LPE (18:3), LPE (18:1) via glycerophospholipid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, glycerolipid metabolism, sphingolipid metabolism, and inositol phosphate metabolism, indicating their potential regulation role in the pathogenesis of fatty liver.
Influence of perceived social support on detection of social norm violation: evidence from N1 and N400
Authors: Liang Bing , Li Bingbing , Fan Xiaoyue , Mu Yan , Wang Juan 
Published: February 2024

IntroductionThe perceived social support individuals receive from their others plays a crucial role in shaping conformity with social norms. However, the specific mechanism underlying perceived social support and the detection of social norms remains unclear.MethodsIn this study, college students with high and low levels of perceived social support were asked to judge the appropriateness of stranger’s behaviors (e.g., singing) in different situations (e.g., library). The participants’ electroencephalography activities were analyzed aiming to uncover the neural mechanism underlying how perceived social support influences the detection of others’ normative behavior.ResultsThe ERP results indicate that, for individuals with a lower level of perceived social support, larger amplitudes of the N1 component (related to primary processing) and the N400 component (related to cognitive conflict) were observed when detecting others’ social norm violation compared to the conformity condition. However, for individuals with a higher level of perceived social support, no significant differences were found in detecting others’ conformity or violation of social norms.DiscussionThe results indicate that, when the perceived social support level of the individual is low, detecting others’ social norm violation elicits deeper primary processing and stronger cognitive conflict compared to conformity condition.
Gender differences in geriatric depressive symptoms in urban China: the role of ADL and sensory and communication abilities
Authors: Guo Ling , Fang Mingwang , Wang Lingying , Liu Li , He Chunxia , Zhou Xiumei , Lu Yi , Hu Xiuying 
Published: February 2024

ObjectivesADL and Sensory and Communication Abilities are important indicators of the quality of life of the elderly which are significant determinants of health, particularly in developing countries. The present cross-sectional study investigated effect of ADL and Sensory and Communication Abilities on depressive symptoms, as well as the the role of gender in these effects.DesignThis is a cross-sectional study.SettingA nationally representative cross-sectional survey among the Chinese population aged 60 years and over.ParticipantsA total of 163296 females and 148724 males aged 65 and over in 2019 in urban China.Outcome measuresPrevalence, risk factors and gender differences in geriatric depressive symptoms among urban elderly.ResultsApproximately 95.69% of the participants had depressive symptoms according to the CESD-10, with no statistically significant gender difference of 52.15% in females and 47.85% in males. Logistic regression findings suggest that geriatric depressive symptoms are significantly associated with the lack of eldercare (OR=2.427, female; OR=1.426, male), living alone(OR= 1.430, female; OR= 1.179, male), ADL dysfunction (OR=1.528, female; OR=1.246, male), and impaired sensory and communication ability (OR=1.338, female; OR=1.185, male) among both female and male participants. Remarkably, geriatric depressive symptoms are only significantly associated with age (≥75, OR = 1.327), marital status (unmarried, OR=1.598), the number of children (no children, OR=2.271), and the living arrangement (living alone, OR= 1.430) among female participants.ConclusionSignificant gender differences in these associations were found for living alone, ADL dysfunction and impaired sensory and communication ability. Moreover, the study emphasized that the gender difference exists in terms of geriatric depression in urban China. Females are more likely to experience depressive than males with the same circumstances.