bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acoustic Levitation Synthesis of Ultrahigh‐Density Spherical Nucleic Acid Architectures for Specific SERS Analysis
Authors: Ding Zhongxiang , Gao Heng , Wang Chao , Li Yuzhu , Li Ning , Chu Leiming , Chen Haijie , Xie Haijiao , Su Mengke , Liu Honglin 
Published: March 2024

AbstractControllably regulating the electrostatic bilayer of nanogold colloids is a significant premise for synthesizing spherical nucleic acid (SNA) and building ordered plasmonic architectures. We develop a facile acoustic levitation reactor to universally synthesize SNAs with an ultra‐high density of DNA strands, which is even higher than those of various state‐of‐the‐art methods. Results reveal a new mechanism of DNA grafting via acoustic wave that can reconfigure the ligands on colloidal surfaces. The acoustic levitation reactor enables substrate‐free three‐dimentional (3D) spatial assembly of SNAs with controllable interparticle nanogaps through regulating DNA lengths. This kind of architecture may overcome the plasmonic enhancement limits by blocking electron tunneling and breaking electrostatic shielding in dried aggregations. Finite element simulations support the architecture with 3D spatial plasmonic hotspot matrix, and its ultrahigh surface‐enhanced Raman scattering (SERS) capability is evidenced by in situ untargeted tracking of biomolecular events during photothermal stimulation (PTS)‐induced cell death process. For biomarker diagnosis, the conjugation of adenosine triphosphate (ATP) aptamer onto SNAs enables in situ targeted tracking of ATP during PTS‐induced cell death process. Particularly, the CD71 receptor and integrin α3β1 protein on PL45 cell membrance could be well distinguished by label‐free SERS fingerprints when using specific XQ‐2d and DML‐7 aptamers, respectively, to synthesize SNA architectures. Our current acoustic levitation reactor offers a new method for synthesizing SNAs and enables both targeted and untargeted SERS analysis for tracking molecular events in living systems. It promises great potentials in biochemical synthesis and sensing in future.
Total Synthesis of Pallamolides A−E
Authors: Zhang Yu , Chen Lijun , Jia Yanxing 
Published: March 2024

AbstractWe have achieved the first total synthesis of pallamolides A−E. Of these compounds, pallamolides B−E possess intriguing tetracyclic skeletons with novel intramolecular transesterifications. Key transformations include highly diastereoselective sequential Michael addition reactions to construct the bicyclo[2.2.2]octane core with the simultaneous generation of two quaternary carbon centers, a one‐pot SmI2‐mediated intramolecular ketyl–enoate cyclization/ketone reduction to generate the key oxabicyclo[3.3.1]nonane moiety, and an acid‐mediated deprotection/oxa‐Michael addition/β‐hydroxy elimination cascade sequence to assemble the tetracyclic pallamolide skeleton. Kinetic resolution of ketone 14 through Corey–Bakshi–Shibata reduction enabled the asymmetric synthesis of pallamolides A−E.
Engineering a Dual‐Functionalized PolyHIPE Resin for Photobiocatalytic Flow Chemistry
Authors: Broumidis Emmanouil , Paradisi Francesca 
Published: March 2024

AbstractThe use of a dual resin for photobiocatalysis, encompassing both a photocatalyst and an immobilized enzyme, brings several challenges, including effective immobilization, maintaining photocatalyst and enzyme activity and ensuring sufficient light penetration. However, the benefits, such as integrated processes, reusability, easier product separation, and potential for scalability, can outweigh these challenges, making dual resin systems promising for efficient and sustainable photobiocatalytic applications. In this study, we employed a photosensitizer‐containing porous emulsion‐templated polymer as a functional support that is used to covalently anchor a chloroperoxidase from Curvularia inaequalis (CiVCPO). We demonstrate the versatility of this heterogeneous photobiocatalytic material, which enables the bromination of four aromatic substrates, including rutin—a natural occurring flavonol—under blue light (456 nm) irradiation and continuous flow conditions.
Multiwalled carbon nanotubes@pectin/κ‐carrageenan‐based nanocomposite biohydrogel prepared by gamma irradiation for efficient methylene blue dye sequestration
Authors: Aboelkhir Doaa M. , Sayed Asmaa , Eldondaity Leila S. , Joseph Veronica , Amin Ayman , Mahmoud Ghada A. 
Published: March 2024

AbstractThis study addresses the critical issue of removing toxic dyes from industrial wastewater to protect the environment and human health. To address this challenge, a bio‐based composite hydrogel was synthesized using gamma irradiation, 20 kGy, incorporating multiwalled carbon nanotubes@pectin/κ‐carrageenan/polyacrylic acid (MWCNTs PC/KC/PAAc) for removing methylene blue (MB) dye from aqueous solutions. The prepared composites underwent characterization through Fourier‐transform infrared spectroscopy, x‐ray diffraction, and thermogravimetric analyses. Brunauer–Emmett–Teller analysis demonstrated a significant increase in surface area upon the addition of MWCNTs. Specifically, the surface area increased from 342.5 m2 g−1 for PC/KC/PAAc to 689.5 m2 g−1 when 0.175 wt% of MWCNTs were added. Also, scanning electron microscopy and atomic force microscopy were utilized to examine the surface topography of PC/KC/PAAc and MWCNTs@PC/KC/PAAc. The results reveal that at 0.175 wt% of MWCNTs, there was a more uniform surface topography with well‐distributed MWCNTs within the matrix. In the adsorption study of MB dye, it was found that the highest MB removal efficiency was achieved at pH 11, with values of 68.21% and 96% for PC/KC/PAAc and MWCNTs@PC/KC/PAAc, respectively. Furthermore, upon studying the effect of MWCNTs (%), the results showed that the removal (%) of PC/KC/PAAc was 68.21 ± 1.02%, and this percentage increased rapidly with the addition of MWCNTs, reaching a maximum of 96 ± 0.85% when 0.175 (wt%) of MWCNTs was added. Isotherm and kinetic modeling demonstrated that MB adsorption follows the Freundlich isotherm for PC/KC/PAAc and the Redlich–Peterson isotherm for MWCNTs@PC/KC/PAAc, both following a pseudo‐second‐order kinetics model. In addition, from AFM data, after MB removal, the height increased significantly from 322 nm for PC/KC/PAAc to 810 nm for MWCNTs@PC/KC/PAAc (0.175 wt%), indicating a substantially higher adsorption of MB by MWCNTs@PC/KC/PAAc.
Harmless photonic crystal tattoo with angle‐independent structural color based on SiO2 nanoparticles and silk fibroin
Authors: Liu Yuxin , Liu Shiqi , Zhu Long , Wu Yun , Si Pengxiang , Zhang Dan 
Published: March 2024

AbstractOwing to the potentially irritant nature of chemical ingredient in cosmetics, harmless photonic crystal tattoo (HPCT) materials with angle‐independent structural color have shown great promise in cosmetic industry. However, it still remains a challenge to produce artificial photonic crystal materials with good adherence to biological cutaneous tissue. Herein, a photonic crystal tattoo based on SiO2 nanoparticles/silk fibroin (SiO2 NPs/SF) is reported. With the SF solution addition of 55.8%, the 180° peel strength and nanoindentation adhesion force of product increased by 427.4% and 103.9%, respectively. Finally, as proof of concept, its applications for creating tattoo with optical pattern on artificial skin were shown. Based on its superior adhesion, harmlessness, and environmental friendliness, the product may be a promising candidate material for the cosmetic industry.
Zero‐sum game for nonlinear multiagent systems with full‐state constraints
Authors: Ji Weiyu , Pan Yingnan , Zhao Meng 
Published: March 2024

AbstractThis paper addresses the zero‐sum game problem for strict‐feedback nonlinear multiagent systems with full‐state constraints. Specifically, this paper focuses on the zero‐sum game scenario, wherein multiple agents aim to optimize the control strategies while considering the conflicting objectives of their opponents. To handle the full‐state constraints, a one‐to‐one nonlinear mapping technique is employed to convert the original strict‐feedback system into a more manageable pure‐feedback system without state constraints. In order to find a Nash equilibrium for virtual control signals and external disturbances, a simplified reinforcement learning algorithm is proposed, which tackles the challenges posed by solving the Hamilton–Jacobi–Isaacs equation. Unlike the existing
optimal control strategies that deal with matching conditions, the
optimal control strategy for strict‐feedback nonlinear systems needs to address the computational complexity issue arising from the repeated derivation of the virtual controller. To overcome the high‐order virtual controller problem, an approach based on the dynamic surface technique is introduced. By incorporating an approximation term of the high‐order virtual controller into the value function, the computational complexity challenge is effectively resolved. Based on the Lyapunov stability theorem, it is proved that all signals of the closed‐loop systems are semi‐global uniformly ultimately bounded and the tracking control performance can be guaranteed. Finally, simulation results are given to verify the effectiveness of the proposed control strategy.
Cost‐effectiveness of Resonance® metallic ureteral stent compared with standard polyurethane ureteral stents in malignant ureteric obstruction: A cost‐utility analysis
Authors: Cooper Dawn M. , Lines Rachel , Shergill Iqbal 
Published: March 2024

AbstractBackgroundMalignant ureteral obstruction (MUO) is a frequent challenge for urologists. Patients have poor prognoses, treatment aims to improve quality‐of‐life while optimising renal function. Standard practice in the United Kingdom is to use polyurethane stents, which require frequent surgical replacements for blockages and encrustation. More durable metallic stents are available, although these incur an increased initial purchase price.AimsWe aim to assess whether the use of polyurethane double‐J (JJ) or metallic stent, Resonance® is more cost‐effective for managing MUO in the UK healthcare setting.MethodsA Markov model was parameterised to 5 years with costs and health‐related quality‐of‐life consequences for treating MUO with Resonance metallic stent (Cook Medical), versus standard JJ stents, from the UK care system perspective, with 3.5% discounting. Deterministic and probabilistic sensitivity analyses were undertaken to assess the effect of uncertainty.ResultsOver 5 years, approximately four fewer repeat surgical interventions were estimated in the metallic stent arm compared with the JJ stent, driving a 23.4% reduction in costs. The mean estimates of costs and benefits indicate that treatment of MUO with Resonance for 5 years is dominant over JJ stents. Over 5 years a cost‐saving of £2164.74 and a health gain of +0.046 quality‐adjusted life years (QALYs) per patient is estimated. With a maximum willingness to pay of £20 k per QALY, a net monetary benefit (NMB) of £3077.83 is estimated. Probabilistic sensitivity analysis at a willingness to pay threshold of £20 000 indicates an 89.3% probability of Resonance being cost‐effective over JJ stents. Within 1‐year savings of £726.53 are estimated driven by a reduction of two fewer repeat surgical interventions when using the metallic stent.ConclusionsResonance metallic stents for the treatment of MUO reduce the number of repeat procedures and could be a cost‐effective option for the treatment, potentially offering efficiencies to the healthcare system.
Application of hydrogel microneedles in the oral cavity
Authors: He Yiyao , He Dawei , Fan Lin , Ren Song , Wang Lin , Sun Jiang 
Published: March 2024

AbstractMicroneedles are a transdermal drug delivery system in which the needle punctures the epithelium to deliver the drug directly to deep tissues, thus avoiding the influence of the first‐pass effect of the gastrointestinal tract and minimizing the likelihood of pain induction. Hydrogel microneedles are microneedles prepared from hydrogels that have good biocompatibility, controllable mechanical properties, and controllable drug release and can be modified to achieve environmental control of drug release in vivo. The large epithelial tissue in the oral cavity is an ideal site for drug delivery via microneedles. Hydrogel microneedles can overcome mucosal hindrances to delivering drugs to deep tissues; this prevents humidity and a highly dynamic environment in the oral cavity from influencing the efficacy of the drugs and enables them to obtain better therapeutic effects. This article analyzes the materials and advantages of common hydrogel microneedles and reviews the application of hydrogel microneedles in the oral cavity.
Evaluation of Orange data mining software and examples for lecturing machine learning tasks in geoinformatics
Authors: Dobesova Zdena 
Published: March 2024

AbstractThe study presents the advantages of, and possible uses for, Orange software for data mining in combination with processing spatial data by ArcGIS Pro software in education. To present suitability of Orange software in education, the scientific method of Physics of Notation by D. Moody is used to evaluate the Orange software's visual vocabulary. All nine principles are applied in the presented evaluation. As a result, a high level of effective cognition of the Orange visual vocabulary is proven by this method. Namely, the semantic transparency of visual vocabulary, thanks the explicit inner icons, is semantically immediate. Also, principle of dual coding is used properly by automatic text labels of graphical symbols with the opportunity to rename labels. Renaming is also a way to ensure the partial overloading of symbols found by the first principle of semiotic clarity. The principle of cognitive interaction is partially fulfilled by automatically reorganizing connector lines between symbols to reduce the crossing of lines. A high level of effective cognition is beneficial for students. The evaluation of the visual notation of Orange software is presented to inform teachers and the geoinformatics community of the highly effective cognitive aspects of Orange software. The two practical lectures of processing in Orange and ArcGIS Pro software are shown to the teachers and students of geoinformatics community as examples of machine learning tasks. They are cluster analyses carried out with the density‐based spatial clustering of applications with noise method, first for the location of cafés in Olomouc town and the second example concerns finding similar European towns based on their land use arrangement, using the neural network and following hierarchical clustering. Both examples could provide inspiration for the geoinformatics community to adopt Orange data mining software.
Lignans Isolated from Piper truncatum Act as New Potent Antitrypanosomal Compounds
Authors: de M. Gonçalves Marina , Totini Carlos H. , De Castro Erica V. , Albuquerque Vanessa , Antar Guilherme M. , Tempone Andre G. , Lago João Henrique G. 
Published: March 2024

AbstractThe hexane extract from twigs of Piper truncatum Vell (Piperaceae) displayed activity against Trypanosoma cruzi and was subjected to chromatographic steps to afford six dibenzylbutyrolactolic lignans, being four knowns: cubebin (1), (−)‐9α‐O‐methylcubebin (2), (+)‐9β‐O‐methylcubebinin (3) and 3,4‐dimethoxy‐3,4‐demethylenedioxycubebin (4) as well as two new, named truncatin A (5) and B (6). Initially, in vitro activity against trypomastigotes was evaluated and compounds 1, 4 and 6 exhibited EC50 values of 41.6, 21.0 and 39.6 μM, respectively. However, when tested against amastigotes, the relevant clinical form in the chronic phase of Chagas disease, compounds 1–6 displayed activities with EC50 values ranging from 1.6 to 13.7 μM. In addition, the mammalian cytotoxicity of compounds 1–6 was evaluated against murine fibroblasts (NCTC). Compounds 2, 3 and 4 exhibited reduced toxicity against NCTC cells (CC50>200 μM), resulting in SI values of>21.9,>14.5 and>121.9, respectively. Compound 4 showed the highest potency with an SI value twice superior to that determined by the standard drug benznidazole (SI>54.6) against the intracellular amastigotes. These data suggest that lignan 4 can be considered a possible scaffold for designing a new drug candidate for Chagas disease.
Establishment and evaluation of detection methods for process‐specific residual host cell protein and residual host cell DNA in biological preparation
Authors: Tian Yixiao , Wang Xinyue , Shao Dongyan , Zhao Wen , Chen Renan , Huang Qingsheng 
Published: March 2024

AbstractTo establish accurate detection methods of process‐specific Escherichia coli residual host cell protein (HCP) and residual host cell DNA (rcDNA) in recombinant biological preparations. Taking the purification process of GLP expressed by E. coli as a specific‐process model, the HCP of empty E. coli was intercepted to immunize mice and rabbits. Using IgG from immunized rabbits as the coating antibody and mouse immune serum as the second sandwich antibody, a process‐specific enzyme‐linked immunosorbent assay (ELISA) for E. coli HCP was established. Targeting the 16S gene of E. coli, ddPCR was used to obtain the absolute copies of rcDNA in samples. Non‐process‐specific commercial ELISA kit and the process‐specific ELISA established in this study were used to detect the HCP in GLP preparation. About 62% of HCPs, which should be process‐specific HCPs, could not be detected by the non‐process‐specific commercial ELISA kit. The sensitivity of established ELISA can reach 338 pg/mL. The rcDNA could be absolutely quantitated by ddPCR, for the copies of rcDNA in three multiple diluted samples showed a reduced gradient. While the copies of rcDNA in three multiple diluted samples could not be distinguished by the qPCR. Process‐specific ELISA has high sensitivity in detecting process‐specific E. coli HCP. The absolutely quantitative ddPCR has much higher accuracy than the relatively quantitative qPCR, it is a nucleic acid quantitative method that is expected to replace qPCR in the future.
Cisplatin‐resistance induces lung squamous carcinoma cell growth by nicotine‐mediated α7nAchR/HDAC1/Cyclin D1/pRb cell cycle activation
Authors: Rao Leh , Guo Dan , Wu Jia‐Ping 
Published: March 2024

AbstractThe majority of adenocarcinoma lung cancer is found in nonsmokers. A history of tobacco use is more common in squamous cell carcinoma of the lung. The aim of this study is to identify the cisplatin (CDDP)‐resistance that promotes lung squamous carcinoma cell growth through nicotine‐mediated HDAC1/7nAchR/E2F/pRb cell cycle activation. Squamous cell carcinoma (NCI‐H520 and NCI‐H157) cells were examined after cisplatin and nicotine treatment by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide assay, cell migration assay, immunofluorescence staining, western blot analysis, and immunoprecipitation analysis. Consequently, CDDP is released from DNA and Rb phosphorylated pRb as a result of nicotine‐induced cancer cell proliferation through 7nAchR, which then triggers the opening of the HDAC1 cell cycle. The cell cycle is stopped when CDDP adducts are present. Nicotine exerts cancer cytoprotective effects by allowing HDAC1 repair mechanisms to re‐establish E2F promoting DNA stimulation cell cycle integrity in the cytosol and preventing potential CDDP and HDAC1 suppressed in the nuclear. Concentration expression of nicotine causes squamous carcinoma cell carcinogens to emerge from inflammation. COX2, NF‐KB, and NOS2 increase as a result of nicotine‐induced squamous carcinoma cell inflammation. Nicotine enhanced the cell growth‐related proteins such as α7nAchR, EGFR, HDAC1, Cyclin D, Cyclin E, E2F, Rb, and pRb by western blot analysis. It also induced cancer cell inflammation and growth. As a result, we suggest that nicotine will increase the therapeutic resistance effects of CDDP. This has the potential to interact with nicotine through α7nAchR receptors and HDAC1/Cyclin D/E2F/pRb potentially resulting in CDDP therapy resistance, as well as cell cycle‐induced cancer cell growth.
Predisposition in Dynamic Covalent Chemistry: The Role of Non‐Covalent Interactions in the Assembly of Tetrahedral Boronate Cages
Authors: Rondelli Manuel , Pasán Jorge , Fernández Israel , Martín Tomás 
Published: March 2024

AbstractDirectional bonding strategies guide the design of complex molecular architectures, yet challenges arise due to emergent behavior. Rigid structures face geometric constraints and sensitivity to mismatches, hindering the efficient assembly of molecular organic cages (MOCs). Harnessing intramolecular non‐covalent interactions offers a promising solution, broadening geometrical possibilities and enhancing adaptability to boost assembly yields. However, identifying these interactions remains challenging, with their full potential sometimes latent until final assembly. This study explores these challenges by synthesizing boronic acid tripods with varied oxygen positions at the tripodal feet and investigating their role in assembling tetrahedral boronate MOCs. Our results reveal substantial differences in the assembly efficiency among tripods. While the building blocks with oxygen in the benzylic position relative to the central aromatic ring form the MOCs in high yields, those with the oxygen atom directly bound to the central aromatic ring, only yield traces. Through X‐ray crystallography and DFT analyses, we elucidate how intramolecular interactions profoundly influence the geometry of the building blocks and cages in a relay‐like fashion, highlighting the importance of considering intramolecular interactions in the rational design of (supra)molecular architectures.
Flexible Full‐Inorganic Ultrathin Films with Stable Circularly Polarized Luminescence Covering the Visible to Near‐Infrared Region
Authors: Wu Wenxuan , Yao Wenyan , Zuo Lulu , Li Xinwei , Yang Xuekang , Liu Yaling , Tang Zhiyong 
Published: March 2024

AbstractCircularly polarized luminescence (CPL) materials hold significant value in various fields, including information storage, secure communication, three‐dimensional displays, biological detection, and optoelectronic devices. Using the Langmuir‐Schaeffer (LS) assembly technique, we successfully construct a series of large‐area flexible optical ultrathin films. Impressively, the inorganic assembled ultrathin films exhibit excellent CPL optical activity covering the visible to near‐infrared (NIR) region, with the luminescence asymmetry factor glum ranging from 0.59 to 0.72. Moreover, such ultrathin films also display outstanding mechanical flexibility, the optical activity of which even after 240 bending cycles shows almost no difference compared to the unbent samples. Owing to the ultra‐broadband optical activity and ultra‐stable optical activity of such full‐inorganic assembled materials on flexible substrates, coupled with their excellent processability and outstanding mechanical flexibility, we anticipate they will find use in many fields such as communication technology and flexible optoelectronics.