bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oligodendrocyte progenitor cells' fate after neonatal asphyxia—Puzzling implications for the development of hypoxic–ischemic encephalopathy
Authors: Janowska Justyna , Gargas Justyna , Zajdel Karolina , Wieteska Michal , Lipinski Kamil , Ziemka‐Nalecz Malgorzata , Frontczak‐Baniewicz Malgorzata , Sypecka Joanna 
Published: March 2024

AbstractPremature birth or complications during labor can cause temporary disruption of cerebral blood flow, often followed by long‐term disturbances in brain development called hypoxic–ischemic (HI) encephalopathy. Diffuse damage to the white matter is the most frequently detected pathology in this condition. We hypothesized that oligodendrocyte progenitor cell (OPC) differentiation disturbed by mild neonatal asphyxia may affect the viability, maturation, and physiological functioning of oligodendrocytes. To address this issue, we studied the effect of temporal HI in the in vivo model in P7 rats with magnetic resonance imaging (MRI), microscopy techniques and biochemical analyses. Moreover, we recreated the injury in vitro performing the procedure of oxygen–glucose deprivation on rat neonatal OPCs to determine its effect on cell viability, proliferation, and differentiation. In the in vivo model, MRI evaluation revealed changes in the volume of different brain regions, as well as changes in the directional diffusivity of water in brain tissue that may suggest pathological changes to myelinated neuronal fibers. Hypomyelination was observed in the cortex, striatum, and CA3 region of the hippocampus. Severe changes to myelin ultrastructure were observed, including delamination of myelin sheets. Interestingly, shortly after the injury, an increase in oligodendrocyte proliferation was observed, followed by an overproduction of myelin proteins 4 weeks after HI. Results verified with the in vitro model indicate, that in the first days after damage, OPCs do not show reduced viability, intensively proliferate, and overexpress myelin proteins and oligodendrocyte‐specific transcription factors. In conclusion, despite the increase in oligodendrocyte proliferation and myelin protein expression after HI, the production of functional myelin sheaths in brain tissue is impaired. Presented study provides a detailed description of oligodendrocyte pathophysiology developed in an effect of HI injury, resulting in an altered CNS myelination. The described models may serve as useful tools for searching and testing effective of effective myelination‐supporting therapies for HI injuries.
Industrial technological progress, technology spillover, and the environment in a dual agricultural economy
Authors: Wu Yunyun , Li Xiaochun 
Published: March 2024

AbstractDeveloping nations typically prioritize economic growth. Thus, industrial technological progress frequently leads to production expansion and subsequent environmental degradation. This paper, however, proposes that in a dual agricultural economy, the progress in industrial production technology does not necessarily degrade the environment when considering technology spillover. Utilizing a general equilibrium model, we conclude that, at the beginning of modern agricultural development, increasing technology spillover rate adversely affects the environment and social welfare. Yet, after the popularization of modern agriculture, increasing this rate can enhance environmental and welfare conditions. Under specific conditions, industrial technological progress might promote environmental and welfare benefits.
Bias in job analysis survey ratings attributed to order effects
Authors: Berenbon Rebecca , McHugh Bridget , Anyidoho Philomina Abena 
Published: March 2024

AbstractWhen respondents complete long surveys, evidence suggests that they may change their response behavior as they become fatigued. The present study examines question order effects in a block‐randomized job analysis survey that collected task ratings (importance, frequency, and needed at certification) for a certification program. Analyses showed that average task ratings were lower for tasks presented later in the survey for both complete and partial responses, though the effect of question ordering was stronger for non‐completers in two rating categories (importance and needed at certification). Additionally, rater variance was lower for questions that appeared later in the survey. The results highlight the value of using randomization to mitigate order effects on survey response quality.
Refractive index dispersion prediction for garnets (A3B2C3X12)
Authors: Drazin John W. , Hay Randall S. 
Published: March 2024

AbstractA model for the refractive index dispersion of garnets was developed by extension of a model for garnet refractive indices at 589 nm wavelength. The cation and anion polarizabilities in garnets were determined as a function of wavelength from dispersion data, and fit to Sellmeier relations. Dispersion predictions were compared to measurements of refractive index between 400 and 5500 nm for 35 garnet compositions. The median prediction error was 0.5% with a maximum error < 4.4%. Sources of error and applications of the predictive method are discussed.
Trends in publication impact of evidence‐based healthcare terminology (2013–2022)
Authors: Bourgault Annette M. , Davis Jean W. , LaManna Jacqueline , Conner Norma E. , Turnage Dawn 
Published: March 2024

AbstractAimsThis article explored the publication impact of evidence‐based healthcare terminology to determine usage and discuss options for low usage terms.BackgroundA plethora of terms describe the scholarship of evidence‐based healthcare. Several terms are synonyms, creating redundancy and confusion. The abundance and overlap of terms may impede the discovery of evidence.DesignThis discursive article explored and discussed publication impact of evidence‐based healthcare terms.MethodsEvidence‐based healthcare terms were identified, and their 10‐year (2013–2022) publication impact was assessed in the CINAHL and Medline databases. A card sort method was also used to identify terms with low usage.ResultsA total of 18/32 terms were included in the review. The terms evidence‐based practice, quality improvement, research and translational research were the most highly published terms. Publication data were presented yearly over a 10‐year period. Most terms increased in publication use over time, except for three terms whose use decreased. Several terms related to translational research have multiple synonyms. It remains unknown whether these terms are interchangeable and possibly redundant, or if there are nuanced differences between terms.ConclusionWe suggest a follow‐up review in 3–5 years to identify publication trends to assess context and terms with continued low publication usage. Terms with persistent low usage should be considered for retirement in the reporting of scholarly activities. Additionally, terms with increasing publication trends should be treated as emerging terms that contribute to evidence‐based healthcare terminology.Implications for NursingConfusion about the use of appropriate terminology may hinder progress in the scholarship of evidence‐based healthcare. We encourage scholars to be aware of publication impact as it relates to the use of specific terminology and be purposeful in the selection of terms used in scholarly projects and publications.
Cholesterol depletion induces mesenchymal properties in oral squamous cell carcinoma cell line
Authors: do Nascimento Rebeca Barros , Cerqueira Paloma Souza Gonçalves , Silva Jamerson Carvalho , Fontes Elisa Kauark , dos Santos Elias Almeida , dos Santos Jean Nunes , Nunes Fábio Daumas , Rodrigues Maria Fernanda Setubal Destro , Paiva Katiúcia Batista Silva , Xavier Flávia Caló de Aquino 
Published: March 2024

AbstractBackgroundCholesterol in cell membranes is crucial for cell signaling, adhesion, and migration. Membranes feature cholesterol‐rich caveolae with caveolin proteins, playing roles in epithelial‐mesenchymal transition and cancer progression. Despite elevated cholesterol levels in tumors, its precise function and the effects of its depletion in oral squamous cell carcinoma remain unclear. The aim of this study was to evaluate the influence of cholesterol depletion in oral squamous cell carcinoma cell line and epithelial‐mesenchymal transition process.MethodsCholesterol depletion was induced on SCC‐9 cells by methyl‐β‐cyclodextrin and cell viability, proliferation, apoptosis, and colony formation capacities were evaluated. Gene and protein expressions were evaluated by reverse transcription polymerase chain reaction (RT‐qPCR) and Western Blot, respectively, and cell sublocalization was assessed by immunofluorescence.ResultsCholesterol depletion resulted in alteration of oral squamous cell carcinoma cell morphology at different concentrations of methyl‐β‐cyclodextrin, as well as decreased cell proliferation and viability rates. Analysis of CAV1 transcript expression revealed increased gene expression in the treated SCC‐9 during the 24 h period, at different concentrations of methyl‐β‐cyclodextrin: 5 , 7.5, 10, and 15 mM, in relation to parental SCC‐9. CAV1 protein expression was increased, with subsequent dose‐dependent decrease. A statistically significant difference was observed in samples treated with 5 mM of methyl‐β‐cyclodextrin (p = 0.02, Kruskal–Wallis test). The immunofluorescence assay showed lower cytoplasmic and membrane labeling intensity in the treated samples for CAV1.ConclusionThese findings indicate the modulation of cholesterol as a possible mechanism underlying the regulation of these molecules and activation of epithelial‐mesenchymal transition in oral squamous cell carcinoma.
Public–private wage differentials in Tunisia: Consistency and decomposition
Authors: Amara Mohamed , Khallouli Wajih , Zidi Faicel 
Published: March 2024

AbstractIn this paper, we estimate and decompose the public–private wage differentials for urban areas, using the 2012 Tunisia urban youth survey. Oaxaca decomposition results suggest that, on average, public sector workers earn more than their private counterparts. Additionally, the results indicate that a substantial part of the conditional gap in urban areas can be attributed to observed characteristics. Human capital, particularly education, are the main reason behind the observed log‐wage advantages. Using unconditional quantile decomposition, our findings reveal that, for urban areas, the discrimination effect becomes more pronounced at the upper quantiles of the wage distribution. Separate analyses by gender and educational levels show that male workers across both sectors receive higher compensation than their female counterparts, with a more pronounced gender gap in private sector. Less educated workers are compensated much more in the public sector than in the private sector, while the wage differential for skilled workers decreases rapidly through the distribution.
Transcriptional chronology reveals conserved genes involved in pennate diatom sexual reproduction
Authors: Audoor Sien , Bilcke Gust , Pargana Katerina , Belišová Darja , Thierens Sander , Van Bel Michiel , Sterck Lieven , Rijsdijk Nadine , Annunziata Rossella , Ferrante Maria Immacolata , Vandepoele Klaas , Vyverman Wim 
Published: March 2024

AbstractSexual reproduction is a major driver of adaptation and speciation in eukaryotes. In diatoms, siliceous microalgae with a unique cell size reduction‐restitution life cycle and among the world's most prolific primary producers, sex also acts as the main mechanism for cell size restoration through the formation of an expanding auxospore. However, the molecular regulators of the different stages of sexual reproduction and size restoration are poorly explored. Here, we combined RNA sequencing with the assembly of a 55 Mbp reference genome for Cylindrotheca closterium to identify patterns of gene expression during different stages of sexual reproduction. These were compared with a corresponding transcriptomic time series of Seminavis robusta to assess the degree of expression conservation. Integrative orthology analysis revealed 138 one‐to‐one orthologues that are upregulated during sex in both species, among which 56 genes consistently upregulated during cell pairing and gametogenesis, and 11 genes induced when auxospores are present. Several early, sex‐specific transcription factors and B‐type cyclins were also upregulated during sex in other pennate and centric diatoms, pointing towards a conserved core regulatory machinery for meiosis and gametogenesis across diatoms. Furthermore, we find molecular evidence that the pheromone‐induced cell cycle arrest is short‐lived in benthic diatoms, which may be linked to their active mode of mate finding through gliding. Finally, we exploit the temporal resolution of our comparative analysis to report the first marker genes for auxospore identity called AAE1‐3 (“Auxospore‐Associated Expression”). Altogether, we introduce a multi‐species model of the transcriptional dynamics during size restoration in diatoms and highlight conserved gene expression dynamics during different stages of sexual reproduction.
Comparison of the Clinical Outcomes between All‐inside and Standard Technique in Anterior Cruciate Ligament Reconstruction with 6‐strand Hamstring Tendon Autograft
Authors: Xu Juncai , Jia Yanfeng , Zhang Boxuan , Wang Xiaofeng , Sun Ran 
Published: March 2024

ObjectiveAll‐inside and standard techniques with 4‐strand hamstrings graft have been widely used in anterior cruciate ligament (ACL) reconstruction. However, the graft diameter of less than 8 mm will significantly increase the rate of surgical failure, and the 6‐strand graft can solve this problem. The purpose of this study is to compare all‐inside ACL reconstruction using suspensory cortical button fixation on both tibia and femur with standard ACL reconstruction using suspensory femoral fixation and a bioabsorbable tibial interference screw with a 6‐strand hamstring tendon autograft in postoperative clinical outcomes.MethodsFrom January 2020 to December 2020, 48 patients performed ACL reconstruction were divided into the all‐side group and the standard group according to the different surgical techniques. Magnetic resonance imaging (MRI) and subjective function scores was used to assess clinical outcomes at least 24 months following ACL reconstruction. MRI was used to measure the value of bone tunnel widening in articular and middle portions. Subjective function scores included the Lysholm knee score, the International Knee Documentation Committee (IKDC) score, the Knee Society Score (KSS) for pain and function, and KT‐1000. The t‐test was used assuming the distribution of the patients which follows the normal distribution and we used non‐parametric tests if these two conditions were not satisfied.ResultsAt the final follow‐up, there were 22 patients in the all‐inside group and 24 patients in the standard group. No significant differences were found with respect to femoral tunnel widening and subjective function scores. However, a significant increase in tibial tunnel widening was found in the middle portion of the standard group (2.25 ± 0.74) compared to the all‐inside group (0.76 ± 0.24) (p < 0.01) and also in the articular portion of the standard group (2.07 ± 0.77) compared to the all‐inside group (1.52 ± 0.54) (p = 0.02). In addition, the value of the KT‐1000 was 1.81 ± 0.45 for the all‐inside group and 2.12 ± 0.44 in the standard group (p = 0.016).ConclusionThe objective stability of the knee was relatively better in the all‐inside group than in the standard group. And tunnel widening after ACL reconstruction was significantly greater in the standard technique when compared to the all‐inside technique on the tibia side.
Engineered poplar for bioproduction of the triterpene squalene
Authors: Bibik Jacob D. , Sahu Abira , Kim Boeun , Unda Faride , Andersen Trine B. , Mansfield Shawn D. , Maravelias Christos T. , Sharkey Thomas D. , Hamberger Björn R. 
Published: March 2024

SummaryBuilding sustainable platforms to produce biofuels and specialty chemicals has become an increasingly important strategy to supplement and replace fossil fuels and petrochemical‐derived products. Terpenoids are the most diverse class of natural products that have many commercial roles as specialty chemicals. Poplar is a fast growing, biomassdense bioenergy crop with many species known to produce large amounts of the hemiterpene isoprene, suggesting an inherent capacity to produce significant quantities of other terpenes. Here we aimed to engineer poplar with optimized pathways to produce squalene, a triterpene commonly used in cosmetic oils, a potential biofuel candidate, and the precursor to the further diversified classes of triterpenoids and sterols. The squalene production pathways were either re‐targeted from the cytosol to plastids or co‐produced with lipid droplets in the cytosol. Squalene and lipid droplet co‐production appeared to be toxic, which we hypothesize to be due to disruption of adventitious root formation, suggesting a need for tissue specific production. Plastidial squalene production enabled up to 0.63 mg/g fresh weight in leaf tissue, which also resulted in reductions in isoprene emission and photosynthesis. These results were also studied through a technoeconomic analysis, providing further insight into developing poplar as a production host.
ACVR and ECVDI consensus statement for teleradiology
Authors: Ziemer Lisa S. , De Haan Constance E. , Cardwell Amy L. , Gambino Jennifer M. , McLear Robert C. , Silverman Sam , Spattini Giliola , Thompson Margret 
Published: March 2024

AbstractIncreased demand for the interpretation of diagnostic images by board‐certified radiologists and profound advancements in technology have led to extremely rapid growth in the field of veterinary teleradiology over the past decade. The aim of this consensus statement is to provide a guideline for best practices for quality and safety in veterinary teleradiology. The statement addresses appropriate image acquisition and transmission, the creation of teleradiology submissions, quality control in teleradiology, and appropriate documentation of imaging reports, as deficiencies in any of these areas may directly affect the standard of patient care. The consensus statement may be used as a guide for radiologists, primary care veterinarians, technicians, and students for the use of teleradiology in practice.
Acute Effects of Local High-Frequency Percussive Massage on Deep Fascial and Muscular Stiffness and Joint Range of Motion in Young Adult Men
Authors: Liu Zijian , Zhong Yicheng , Maemichi Toshihiro , Zhou Qianhui , Okunuki Takumi , Li Yanshu , Kazuki Wakamiya , Kumai Tsukasa 
Published: March 2024

Background: Local high-frequency percussive (HFP) massage has recently found widespread application in physical therapy. Although HFP massage reportedly improves range of motion (ROM), the mechanism underlying its action has not yet been proven. This study aimed to clarify whether a 5-minute percussive massage regimen affects muscular or connective tissues, such as the deep fascia and deep intermuscular fascia and the change in joint ROM. Method: The study sample was calculated using G*Power analysis program, and this study enrolled 15 healthy men who underwent 5-minute HFP massage to the medial gastrocnemius muscle. Shear-wave elastography was used to measure tissue stiffness in the deep fascia, muscle, and deep intermuscular fascia through shear-wave velocity as well as the ROM of the volunteers’ ankle joint dorsiflexion before and after the HFP massage. A value of P < .05 was used to declare statistical significance, and post hoc was used to calculate the effect size using G*Power. Results: Shear-wave velocity revealed a significant change in the deep fascia (P = .003; shear-wave velocity: −0.7 m/s) and significant increase in ROM of ankle dorsiflexion (P = .002; increase in ROM: 3.0°) after 5 minutes of HFP massage. However, the muscle and deep intermuscular fascia did not exhibit any significant changes. Conclusions: HFP massage for 5 minutes modified the stiffness of the deep fascia and concurrently improved the ankle joint-dorsiflexion ROM. This method can be used as an intervention to decrease stiffness of the deep fascia and increase the ROM efficiently.
Stratigraphy and depositional history of the Aguja Formation (Upper Cretaceous, Campanian) of West Texas, southwestern USA
Authors: Lehman Thomas M. , Wick Steven L. , (Charlie) Macon Craig C. , Wagner Jonathan R. , Waggoner Karen J. , Brink Alyson A. , Shiller Thomas A. 
Published: March 2024

Abstract
Although the Aguja Formation (West Texas, southwestern USA) and its fossil vertebrate fauna have been known for over a century, its basic stratigraphic requisites (type area and type section) have not been formally documented. The formation is herein subdivided into a series of formal members, and a lectostratotype section is proposed. Lithostratigraphic and biostratigraphic subdivisions are documented and integrated with geochronologic data to provide an age model for the formation. Four terrestrial vertebrate biozones are proposed. There are at least four major depositional intervals represented in the Aguja and intertonguing Pen Formations. An initial progradational deltaic succession is recorded by the La Basa Sandstone and lower part of the Abajo Shale Members of the Aguja Formation. A second phase of deposition resulted in a retrogradational shoreface succession that includes the upper part of the Abajo Shale, overlying Rattlesnake Mountain Sandstone Member, and lower part of the McKinney Springs Tongue of the Pen Formation, up to a skeletal phosphate bed interpreted to represent the maximum flooding surface. The third phase of deposition comprises a progradational deltaic succession that includes the upper part of the McKinney Springs Tongue, Terlingua Creek Sandstone Member of the Aguja Formation, and lower part of the Alto Shale Member of the Aguja Formation. This third succession records eastward migration of the strandline and withdrawal of the Western Interior Seaway from the Big Bend region. The fourth phase of deposition comprises a series of aggradational fluvial channel and floodplain successions that form the upper part of the Alto Shale Member and is coincident with redirection of stream flow to the southeast. This interval is much thicker in the central part of the Big Bend region, thins to the southwest and northeast, and likely records initial subsidence in the Laramide Tornillo Basin. The upper part of this succession was also contemporaneous with a series of basaltic pyroclastic eruptions, the westernmost expression of the Balcones igneous province. A dramatic constriction in the southern entrance to the Western Interior Seaway through the Gulf of Mexico occurred during this final phase in deposition of the Aguja Formation and corresponds to a shift of stream flow southeastward and to an outbreak of local pyroclastic eruptions. Regional uplift associated with this episode of magmatism is likely responsible for closing the southern aperture of the Western Interior Seaway.