bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional proteomic view of metabolic regulation in “Aromatoleum aromaticum” strain EbN1
Authors: Wöhlbrand Lars , Kallerhoff Birte , Lange Daniela , Hufnagel Peter , Thiermann Jürgen , Reinhardt Richard , Rabus Ralf 
Published: June 2007

AbstractThe denitrifying “Aromatoleum aromaticum” strain EbN1 utilizes a wide range of aromatic and nonaromatic compounds under anoxic and oxic conditions. The recently determined genome revealed corresponding degradation pathways and predicted a fine‐tuned regulatory network. In this study, differential proteomics (2‐D DIGE and MS) was used to define degradation pathway‐specific subproteomes and to determine their growth condition dependent regulation. Differential protein profiles were determined for cultures adapted to growth under 22 different substrate and redox conditions. In total, 354 different proteins were identified, 199 of which displayed significantly changed abundances. These regulated proteins mainly represented enzymes of the different degradation pathways, and revealed different degrees of growth condition specific regulation. In case of three substrate conditions (e.g. phenylalanine, anoxic), proteins previously predicted to be involved in their degradation were apparently not involved (e.g. Pdh, phenylacetaldehyde dehydrogenase). Instead, previously not considered proteins were specifically increased in abundance (e.g. EbA5005, predicted aldehyde:ferredoxin oxidoreductase), shedding new light on the respective pathways. Moreover, strong evidence was obtained for thus far unpredicted degradation pathways of three hitherto unknown substrates (e.g. o‐aminobenzoate, anoxic). Comparing all identified regulated and nonregulated proteins provided first insights into regulatory hierarchies of special degradation pathways versus general metabolism in strain EbN1.
A high‐throughput strategy for protein profiling in cell microarrays using automated image analysis
Authors: Strömberg Sara , Björklund Marcus Gry , Asplund Caroline , Sköllermo Anna , Persson Anja , Wester Kenneth , Kampf Caroline , Nilsson Peter , Andersson Ann‐Catrin , Uhlen Mathias , Kononen Juha , Ponten Fredrik , Asplund Anna 
Published: June 2007

AbstractAdvances in antibody production render a growing supply of affinity reagents for immunohistochemistry (IHC), and tissue microarray (TMA) technologies facilitate simultaneous analysis of protein expression in a multitude of tissues. However, collecting validated IHC data remains a bottleneck problem, as the standard method is manual microscopical analysis. Here we present a high‐throughput strategy combining IHC on a recently developed cell microarray with a novel, automated image‐analysis application (TMAx). The software was evaluated on 200 digital images of IHC‐stained cell spots, by comparing TMAx annotation with manual annotation performed by seven human experts. A high concordance between automated and manual annotation of staining intensity and fraction of IHC‐positive cells was found. In a limited study, we also investigated the possibility to assess the correlation between mRNA and protein levels, by using TMAx output results for relative protein quantification and quantitative real‐time PCR for the quantification of corresponding transcript levels. In conclusion, automated analysis of immunohistochemically stained in vitro‐cultured cells in a microarray format can be used for high‐throughput protein profiling, and extraction of RNA from the same cell lines provides a basis for comparing transcription and protein expression on a global scale.
Electron‐pair‐donor reaction order in the cationic polymerization of isobutylene coinitiated by AlCl3
Authors: Li Yan , Wu Yixian , Xu Xu , Liang Lihu , Wu Guanying 
Published: June 2007

AbstractThe cationic polymerizations of isobutylene (IB) initiated by an H2O/AlCl3 system were carried out in a mixture of n‐hexane/methylene dichloride of 60/40 v/v at −50 °C in the presence of various external electron pair donors (EDs), including methyl benzoate (MB), ethyl benzoate (EB), and methyl acrylate (MA). The effects of the concentrations of EDs ([ED]) and polymerization time on monomer conversion and kinetics of IB polymerization were also investigated. The complexes between AlCl3 and the esters were soluble in the polymerization system at −50 °C. The polymers with high molecular weights and relatively narrow molecular weight distributions were obtained in the presence of the EDs. The rate of polymerization decreased with increasing [ED]. The kinetic orders of the EDs were remarkably dependent on their chemical structure, steric hindrance from moieties, and concentration in the polymerization system. The reaction order of MB was determined to be around −1.3 when [MB] ≤ 0.40 mmol/L, whereas it was −12.9 when [MB] ≥ 0.40 mmol/L. The reaction order of EB was determined to be −1.36 when [EB] ≤ 0.41 mmol/L, whereas it was −3.36 when [EB] ≥ 0.41 mmol/L. The reaction order of MA was determined to be −1.85 when [MA] ≤ 0.48 mmol/L, whereas it was −16.7 when [MA] ≥ 0.48 mmol/L. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 3053–3061, 2007
Synthesis and morphology of an iron oxide/polystyrene/poly(isopropylacrylamide‐co‐methacrylic acid) thermosensitive magnetic composite latex with potassium persulfate as the initiator
Authors: Lee Chia‐Fen , Chou Yu‐Hui , Chiu Wen‐Yen 
Published: June 2007

AbstractIn this work, an iron oxide (Fe3O4)/polystyrene (PS)/poly(N‐isopropylacryl amide‐co‐methacrylic acid) [P(NIPAAM–MAA)] thermosensitive magnetic composite latex was synthesized by the method of two‐stage emulsion polymerization. The Fe3O4 particles were prepared by a traditional coprecipitation method and then surface‐treated with either a PAA oligomer or lauric acid to form a stable ferrofluid. The first stage for the synthesis of the thermosensitive magnetic composite latex was to synthesize PS in the presence of a ferrofluid by emulsion polymerization to form Fe3O4/PS composite latex particles. Following the first stage of reaction, the second stage of polymerization was carried out with N‐isopropylacryl amide and methacrylic acid as monomers and with Fe3O4/PS latex as seeds. The Fe3O4/PS/[P(NIPAAM–MAA)] thermosensitive magnetic particles were thus obtained. The effects of the ferrofluids on the reaction kinetics, morphology, and particle size of the latex were discussed. A reaction mechanism was proposed in accordance with the morphology observation of the latex particles. The thermosensitive property of the thermosensitive magnetic composite latex was also studied. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 3062–3072, 2007
Psychiatric morbidity and quality of life in wives of men with long‐term head and neck cancer
Authors: Drabe Natalie , Zwahlen Diana , Büchi Stefan , Moergeli Hanspeter , Zwahlen Roger A. , Jenewein Josef 
Published: June 2007

AbstractThe diagnosis of cancer not only affects the lives of patients but also the lives of their relatives. The aim of this study was to investigate the prevalence of psychiatric disorders and quality of life (QoL) among wives of patients treated for head and neck cancer (HNC). We examined 31 wives of patients treated for HNC from January 1998 to December 2004 (meantime since diagnosis 3.7 years) by questionnaires with regard to quality of life (WHOQOL‐BREF), quality of the relationship (Dyadic Adjustment Scale), and affective symptoms (Hospital Anxiety and Depression Scale, HADS). Prevalence of psychiatric morbidity was measured by the Mini International Neuropsychiatric Interview (MINI). Results indicated that QoL and satisfaction with the relationship were comparable to the normal population. HADS mean scores showed no clinically relevant levels of depression and anxiety. A high prevalence of psychiatric disorders (38.7%, particularly agoraphobia) was found in the MINI. Wives diagnosed with an anxiety disorder reported significantly lower QoL compared to those without. The results of this study suggest that agoraphobia is a frequent psychiatric disorder seen in wives of HNC patients. Diagnosis of HNC can have a strong impact on the mental health of the spouse and should be taken in account in counselling of HNC patients. Copyright © 2007 John Wiley & Sons, Ltd.
Complaint handling in pharmaceutical companies
Authors: Braga Glaucia Karime 
Published: June 2007

AbstractComplaints show customer dissatisfaction about the quality of a pharmaceutical product. Despite a regulatory obligation in several countries, a good complaint handling system gives the company an opportunity to improve the quality of their products, being a good tool for the maintenance of Good Manufacturing Practices and a way to establish a committed relationship with their customers. A systematic procedure must be developed and implemented in order to register and investigate each complaint received and not only the Quality Assurance area must be involved. This process must involve other critical areas such as Marketing, Production, Quality Control, Finance, Regulatory and Legal Affairs. Thus the aim of this article is to discuss the main steps of a good complaint handling procedure that can be readily implemented in pharmaceutical companies. Copyright © 2007 John Wiley & Sons, Ltd.
The JSQA guideline for GCP auditing
Authors: Shimokai Hiroshi , Hata Seiichi , Tamura Toshiaki , Yano Yoshiaki , Abe Shu , Takezawa Masayuki , Yogo Masahiro , Kajiho Tokuaki , Gotou Kuniko , Izawa Akio , Ookawa Muneyoshi , Yamashita Hiroyasu , Alaya Fumiko , Shiromoto Yukiko , Takahashi Kiyoshi 
Published: June 2007

AbstractThe International Conference on Harmonisation (ICH) Good Clinical Practice (GCP) has been adopted by three regions USA, Europe and Japan and implemented since 1996. Details of GCP auditing are not provided in ICH GCP. The Japan Society of Quality Assurance (JSQA) has prepared ‘The JSQA GCP Guideline for GCP Auditing’ to promote the global discussion on GCP auditing and expects to establish a global guideline on GCP auditing along with ICH GCP.The guideline will include the mission and the organization of a sponsor's auditing department and establish the principles for planning, performing, and reporting audits. The guideline is expected to be a basic principle along with ICH GCP for not only sponsor's auditors, but also independent auditors and auditors of Contract Research Organizations (CROs) to conduct an audit in the various situations of each country and sponsor. Copyright © 2007 John Wiley & Sons, Ltd.
Ab initio QM calculation of the electric field convergence versus atomic basis sets in periodic models of proton‐substituted zeolites
Authors: Larin A. V. , Trubnikov D. N. , Vercauteren D. P. 
Published: June 2007

AbstractElectrostatic potential (EP) and electric field (EF) values are calculated in periodic models of proton‐substituted brewsterite (HBRE) and chabazite (HCHA) zeolite forms using the periodic DFT (PDFT) hybrid B3LYP level with 13 different basis sets. Relative root mean square differences between the EP, or EF, values estimated between the different basis sets are evaluated in several spatial domains available for adsorbate molecules in both zeolites. In these areas, the EF increase in HCHA is evaluated relative to all‐siliceous CHA. The EP is interpreted in terms of ionicity of the framework. © 2007 Wiley Periodicals, Inc. Int J Quantum Chem, 2007
Polarons in one‐dimensional molecular chains with intermolecular and intramolecular vibrations
Authors: Mborong V. B. , Dikandé A. M. 
Published: June 2007

AbstractPolarons are investigated in a model for one‐dimensional molecular chains involving both acoustical and optical lattice vibrations, as found in diatomic chains. With the help of a specific ansatz for low‐lying quasiparticles, a continuum limit approximation sustains the existence of analytical solutions to the model. The dispersion energy as well as the effective mass of low‐lying electronic states in the presence of the two lattice mode‐induced polaronic quasiparticle, are derived analytically. It is found that the band effective mass is strongly enhanced by a factor depending on the two electron–lattice coupling constants. A test of stability of the analytical shapes of the three long‐wavelength excitations is carried out numerically by following their simultaneous propagation throughout the molecular lattice. The long‐wavelength polaron appears to be very stable within an acceptable range of values of the electron wave vector and propagates faster and faster as one moves from the groundstate toward mid‐band states. However, the accompanying kink and pulse soliton deformations are always slower consistently with the adiabatic considerations underlying the quasi‐classical treatment followed in this work. In addition, the kink component of the lattice deformation tends to become unstable at relatively large electronic wave vectors, while the polaron and in turn the optical lattice deformation are more and more stable, traducing dominant optical modes of the lattice in the process of formation of polaron. © 2007 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Cortical functional network organization from autoregressive modeling of local field potential oscillations
Authors: Bressler Steven L. , Richter Craig G. , Chen Yonghong , Ding Mingzhou 
Published: June 2007

AbstractA framework is presented for quantifying functional network organization in the brain by spectral analysis based on autoregressive modeling. Local field potentials (LFPs), simultaneously recorded from distributed sites in the cerebral cortex of monkeys, are treated as signals generated by local neuronal assemblies. During the delay period of a visual pattern discrimination task, oscillatory assembly activity is manifested in the LFPs in the beta‐frequency range (14–30 Hz). Coherence analysis has shown that these oscillations are phase synchronized in functional networks in the sensorimotor cortex in relation to maintenance of contralateral hand position, and in the visual cortex in relation to anticipation of the visual stimulus. Granger causality analysis has revealed information flow in the sensorimotor network that is consistent with a peripheral sensorimotor feedback loop, and in the visual network that is consistent with top‐down anticipatory modulation of assemblies in the primary visual cortex. Copyright © 2007 John Wiley & Sons, Ltd.
A comparative study of one‐level and two‐level semiparametric estimation of hemodynamic response function for fMRI data
Authors: Zhang C. M. , Jiang Y. , Yu T. 
Published: June 2007

AbstractFunctional magnetic resonance imaging (fMRI) is emerging as a powerful tool for studying the process underlying the working of the many regions of the human brain. The standard tool for analyzing fMRI data is some variant of the linear model, which is restrictive in modeling assumptions. In this paper, we develop a semiparametric approach, based on the cubic smoothing splines, to obtain statistically more efficient estimates of the underlying hemodynamic response function (HRF) associated with fMRI experiments. The hypothesis testing of HRF is conducted to identify the brain regions which are activated when a subject performs a particular task. Furthermore, we compare one‐level and two‐level semiparametric estimates of HRF in significance tests for detecting the activated brain regions. Our simulation studies demonstrate that the one‐level estimates combined with a bias‐correction procedure perform best in detecting the activated brain regions. We illustrate this method using a real fMRI data set and compare it with popular methods offered by AFNI
and FSL. Copyright © 2007 John Wiley & Sons, Ltd.
Control of helix sense in protein‐mimicking backbone by the noncovalent chiral effect
Authors: Inai Yoshihito , Komori Hisatoshi , Ousaka Naoki 
Published: June 2007

AbstractWe have reviewed our previous work regarding induction or control of a peptide helix sense through chiral stimulus to the peptide chain terminus. An optically inactive 310‐helix designed mainly with unusual α‐amino acid residues was commonly employed. Such an N‐terminal‐free peptide generates a preferred helix sense by chiral acid molecule. A helix sense pre‐directed in chiral sequence is also influenced or controlled by the chiral sign of such external molecule. Here free amide groups in the 310‐helical N‐terminus participate in the formation of a multipoint coordinated complex. The terminal asymmetry produces the noncovalent chiral domino effect (NCDE) to influence the whole helix sense. The NCDE‐mediated control of helicity provides the underlying chiral nature of protein‐mimicking helical backbones: notably, chiral recognition at the terminus and modulation of helical propensity through chiral stimulus. The above items from our previous reports have been outlined and reviewed together with their significance in biopolymer science and chiral chemistry. © 2007 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 7: 191–202; 2007: Published online in Wiley Inter‐Science (www.interscience.wiley.com) DOI 10.1002/tcr.20116
Lösungsmöglichkeiten des Umkehrproblems viskoelastischer Cosseratflächen
Authors: Rothert H. 
Published: June 2007

AbstractAusgehend von den konstitutiven Gleichungen für viskoelastische zweidimensionale Cosseratkontinua im Rahmen einer linearen nichtisothermen Theorie, werden zunächst durch Spezialisierung und Ausnutzung von Materialsymmetrien vereinfachte Beziehungen gewonnen. Um, diese Ergebnisse der direkten Theorie für konkrete Platten und Schalen interpretieren zu können, bedarf es der Lösung des Umkehrproblems. Hier werden zur Durchführung des Identifizierungsprozesses die in ihrem mathematischen Aufbau analogen Ergebnisse der dreidimensionalen Boltzmannschen Elastizitätstheorie und das Theorem von Alfrey verwendet.
Das Druckstörungsfeld im Überschall längs einer schwach welligen Wand unter Berücksichtigung der Grenzschicht
Authors: Stock H. W. 
Published: June 2007

AbstractDruckstörungen, erzeugt durch eine wellige Wand bei Überschallanströmung, werden mit der linearisierten Theorie der kleinen Störungen unter Einbeziehung der Grenzschicht berechnet. Dabei wird die Grenzschicht in eine reibungsfreie, rotationelle Außenschicht und eine viskose Unterschicht aufgeteilt. Laminare und turbulente Grenzschichten werden untersucht. Die Druckstörungen, d. h. ihre Amplituden und Phasenlagen, werden ins Verhältnis gesetzt zu den nach der reibungsfreien Theorie ermittelten. Es zeigt sich, daß der Einfluß der Grenzschicht vernachlässigt werden kann, wenn die Wellenlänge der Wandstörung sehr viel größer ist als die Grenzschichtdicke. Für kleinere Wellenlängen wirkt die Grenzschicht dämpfend auf die Außenströmung, d. h. die Amplituden der Druckstörungen sind kleiner als die im reibungslosen Fall gerechneten. Besonders im Falle laminarer Grenzschichten und kleinerer Wellenlängen der Wandstörung nehmen die Druckstörungen in Wandnähe Unterschallcharakter an, obwohl die Außenströmung Überschallgeschwindigkeit hat.
Über eine Beziehung zwischen komplementären und nichtkonformen finiten Elementen
Authors: Werner Bodo 
Published: June 2007

AbstractWir untersuchen die Konstruktion von speziellen Finiten‐Element‐Räumen, die konform für das komplementäre Energieprinzip für allgemeine lineare elliptische Randwertaufgaben sind. Die mit diesen Räumen arbeitenden Finiten‐Element‐Methoden stellen sich als nichtkonforme Methode für das Energieprinzip heraus und erfüllen einen verallgemeinerten Patch‐Test. Von einigen bekannten speziellen nichtkonformen finiten Elementen kann auf diese Weise gezeigt werden, daß sie untere Schranken für die Energie liefern.
Zur globalen Konvergenz eines modifizierten Kato‐Fujita‐Iterationsverfahrens
Authors: Gajewski Herbert 
Published: June 2007

AbstractIn dieser Arbeit beweisen wir einen globalen Konvergenzsatz für eine einfache Variante des sogenannten Kato‐Fujita‐Verfahrens [1,8] zur iterativen Lösung der Burgers‐Gleichung. Unsere Ergebnisse basieren auf a priori Abschätzungen und lassen sich auf andere nichtlineare Evolutionsgleichungen mit linearem Dissipations‐ oder Dispersionsterm übertragen.
Zur Theorie des quasi‐statischen Verhaltens von elastisch‐plastischen Körpern
Authors: Gröger K. 
Published: June 2007

AbstractIn dieser Arbeit wird gezeigt, daß gewisse Modelle zur Beschreibung des Verhaltens von elastisch‐plastischen Körpern als Evolutionsungleichungen formuliert werden können. Ausgehend von solchen Modellen kann man die quasi‐statische Verformung von dreidimensionalen Körpern bei gegebenen äußeren Kräften durch Rand‐Anfangswertaufgaben charakterisieren. Für ein spezielles Materialgesetz wird die eindeutige Lösbarkeit derartiger Rand‐Anfangswertaufgaben bewiesen.
Die Hecubabewegung im eingeschränkten Dreikörperproblem als Beispiel einer nichtlinearen Schwingung
Authors: Mettler E. 
Published: June 2007

AbstractIm Rahmen des eingeschränkten Dreikörperproblems werden die Bewegungen eines Planetoiden mit etwa halber Jupiter‐Umlaufzeit als kleine Schwingungen um eine stationäre Kreisbewegung dargestellt. Dabei wird die Methode der säkularen Störungen benutzt. Es wird gezeigt, daß die Bewegungen die charakteristischen Merkmale nichtlinearer Schwingungen mit Zwangs‐ und Parametererregung und unterlinearer Rückstellkraft haben. Im einzelnen ergeben sich stabile und instabile stationäre Schwingungen sowie Übergangsschwingungen, die den periodischen sowie den Librationslösungen des eingeschränkten Dreikörperproblems entsprechen.
Heat Transfer in a Laminar Cyclone II
Authors: Millsaps K. , Nydahl J. E. 
Published: June 2007

AbstractThis paper is the second and concluding part of an investigation into the heat transfer to rotating laminar flows over fixed ground when the effects due to a combination of forced and free convections are included. The analytical methods that are employed are similarity transformations and subsequent integration of the ordinary differential system by continuously joining second and third order asymptotic approximations with forward numerical integrations that were obtained by perturbing the initial conditions for the solutions for forced convection alone. Numerical results for the thermal distribution and for the hydrodynamics that differ from that the forced convetive case due to coupling are tabulated for two Grashof numbers and a fixed Prandtl number.
Mathematisches Modell und Lösung für die Schmierfilmdicke bei elastohydrodynamischem Kugelkontakt
Authors: Weber C. , Adams E. 
Published: June 2007

AbstractDie durch O. Reynolds (1886) begründete klassische hydrodynamische Schmiertheorie führt u. a. im Fall der hier behandelten Punktberührung auf ein nicht akzeptables Ergebnis: Die durch Kapitza [10] angegebene Lösung für den Fall des Kugelkontaktes liefert das Verschwinden des Integrals über die Druckverteilung und damit auch der Traglast. Es wird hier gezeigt, daß diese Lösung eindeutig ist. Entgegen experimentellen Beobachtungen [20] ergibt die elastohydrodynamische Behandlung des Problems in [2] und in [3] negative Drucke mit nicht vernachlässigbar kleinem Betrag auf der Abströmseite. Dieser Mangel wird hier durch eine physikalisch begründete Abänderung des Freigliedes der Reynoldsschen Gleichung vermieden. Das dabei benutzte mathematische Modell beschreibt die elastische Verformung näherungsweise durch Annahme eines kreisförmigen Hertzschen Spaltes konstanter Filmdicke h0, außerhalb dessen die beiden festen Kugeln unverformt sind. Damit wird die Druckverteilung im inkompressiblen isothermen Schmierfilm überwiegend explizit sowie mit Fehlerabschätzung einer Differenzenlösung bestimmt. Durch Übergang zur Grenze großer Lasten ergibt sich hieraus die Parameterabhängigkeit von h0 in größerer Allgemeinheit als in [2] oder in [3].