bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction and characterization of a functional variant hFGF7 with enhanced properties by circular permutation
Authors: Choi Hye‐Ji , Lee Hanui , Cheong Dae‐Eun , Yoo Su‐Kyoung , Lee Dong‐Eun , Kim Geun‐Joong 
Published: March 2024

AbstractHuman fibroblast growth factor 7 (hFGF7) is a member of the paracrine‐acting FGF family and mediates various reactions such as wound healing, tissue homeostasis, and liver regeneration. These activities make it a plausible candidate for pharmaceutical applications as a drug. However, the low expression level and stability of the recombinant hFGF7 were known to be major hurdles for further applications. Here, the expression level and stability of hFGF7 were attempted to improve by changing the order of amino acids through circular permutation (CP), thereby expecting an alternative fate according to the N‐end rule. CP‐hFGF7 variants were constructed systematically by using putative amino acid residues in the loop region that avoided the disruption of the structural integrity especially in the functional motif. Among them, cp‐hFGF7115‐114 revealed a relatively higher expression level in the soluble fraction than the wild‐type hFGF7 and was efficiently purified (7 mg L−1) to apparent homogeneity. The activity and stability of the purified variant cp‐hFGF7115‐114 were comparable or superior to that of the wild‐type hFGF7, thereby strongly suggesting that CP could be an alternative tool for the functional expression of hFGF7 in Escherichia coli.
Effect of extruded wheat bran on the volatility and physical–chemical properties of bread during frozen storage
Authors: Jia Xiwu , Chen Zhihan , Pan Qinmin , Wang Zhan , Guo Cheng , Jin Weiping , Shen Wangyang 
Published: March 2024

AbstractBackground and ObjectivesAt present, there are no sufficient data in the literature about the effect of extruded wheat bran on the volatility and physical–chemical properties of bread during frozen storage. Therefore, the current study aimed to investigate the effect of extruded wheat bran on the volatile components and physical–chemical properties of bread during frozen storage.FindingsFrozen storage could effectively extend the shelf‐life of bread. After 20 days of storage, the specific volume and cohesion of the control breads decreased significantly (2% and 21.55%, respectively). Moreover, the hardness and amylopectin retrogradation degree increased significantly (81.56%). After 30 days of storage, the amylopectin retrogradation degree of extruded wheat bran breads significantly increased (161%). After 40 days of storage, the cohesion of extruded wheat bran breads decreased significantly (7.67%). Extruded bran was conducive for improving bread stability during frozen storage. The transverse relaxation times (T2) of extruded wheat bran bread were shorter than those of the control bread. Bran inhibited water molecule migration and reduced the rate of bread quality deterioration. Further, 27 volatile components were detected in the control bread and 32 volatile components were detected in extruded wheat bran breads. The volatile components of all breads did not change significantly within the first 30 days of storage. Thereafter, the concentration of some volatile compounds decreased.ConclusionThe addition of extruded wheat bran significantly affected the volatility and physical–chemical properties of bread during frozen storage. The volatility and physical–chemical properties of extruded wheat bran bread changed gradually during frozen storage. The presence of bran inhibited water molecule migration and the formation and recrystallization of large ice crystals, and reduced the rate of bread quality deterioration during frozen storage.Significance and NoveltyThis study aimed to provide a reference for research on the storage and long‐term preservation of extruded wheat bran bread after production.
Dissociative Electron Transfer to N‐(Arylthio)phthalimides: Factors Affecting the Formation and Dissociation of Radical Anions
Authors: Saley Michael A. , Hamed Emad M. , Houmam Abdelaziz 
Published: March 2024

AbstractA series of chemically and biologically relevant N‐(arylthio)phthalimides is synthesized and its electrochemical reduction investigated. The results of the electrochemical study are analysed through application of Savéant's dissociative electron transfer theory and with the assistance of theoretical calculations. Reduction of all investigated N‐(arylthio)phthalimides leads to the corresponding radical anions, followed by the ejection of the phthalimide anion resulting from the dissociation of the N−S chemical bond. This stepwise electron transfer mechanism is evidenced by the electrochemical data which allow determination of the standard reduction potentials of all compounds. In the formation of the N‐(arylthio)phthalimide radical anions, the theoretical calculations show that the incoming electron, is hosted by the phthalimidyl group indicating a homolytic dissociation mechanism. The dissociation rate constants of the radical anions are deduced and show that upon changing the substituent from the MeO, to Me, H, F, Cl and Br the dissociation becomes faster. A good correlation with the Hammett substituent constants is also obtained. Application of Savéant's theory through determination of the associated driving force and the intrinsic barrier energies help rationalize the observed results and show that the change in the dissociation rate constant depends mainly on the standard reduction potential of the parent compounds.
Chemodivergent Synthesis of Benzofurans and 2,3‐Dihydrobenzofurans via Tandem Oxidative Annulation of Enaminones and Salicylaldehydes
Authors: Duan Xiyan , Li Hui , Wang Junqi , Liu Kun , Shi Meixin , Lian Weidong , Chen Ran , Liu Pu 
Published: March 2024

Comprehensive SummaryChemodivergent synthesis of benzofurans and 2,3‐dihydrobenzofurans has been realized. Under a reaction system consisting of DBDMH and K2CO3 as promotors, controlled conditions enabled the formation of two sets of valuable heterocycles from the tandem transformation of enaminones and salicylaldehydes. The key to success was the identification of the reaction parameters, in which the imine intermediate which was formed by transient halogenation coupling and substitution processes underwent either aldol condensation/annulation or imine hydrolysis/aldol condensation. The additives NH4Cl or Fe2(SO4)3 controlled the unique selectivity of this reaction. A broad substrate scope of enaminones and salicylaldehydes has been employed in this reaction, demonstrating excellent functional group tolerance and versatility.
Synthesis of High Entropy Alloy Nanoparticles by Pulsed Laser Ablation in Liquids: Influence of Target Preparation on Stoichiometry and Productivity
Authors: Tahir Shabbir , Shkodich Natalia , Eggert Benedikt , Lill Johanna , Gatsa Oleksandr , Flimelová Miroslava , Adabifiroozjaei Esmaeil , Bulgakova Nadezhda M. , Molina‐Luna Leopoldo , Wende Heiko , Farle Michael , Bulgakov Alexander V. , Doñate‐Buendía Carlos , Gökce Bilal 
Published: March 2024

AbstractHigh entropy alloys (HEAs) have a wide range of applications across various fields, including structural engineering, biomedical science, catalysis, magnetism, and nuclear technology. Nanoscale HEA particles show promising catalytic properties. Nevertheless, attaining versatile composition control in nanoparticles poses a persistent challenge. This study proposes the use of pulsed laser ablation in liquids (PLAL) for synthesizing nanoparticles using equiatomic CoCrFeMnNi targets with varied preparation methods. We evaluate the impact of target preparation method on nanoparticle yield and composition as well as the magnetic properties of the nanoparticles. The elemental powder‐pressed heat‐treated target (HEA‐PP), identified as the most time‐efficient and cost‐effective, exhibits noticeable segregation and non‐uniform elemental distribution compared to ball milled hot‐pressed powder (HEA‐BP) and face‐centered cubic (FCC) single crystal (HEA‐SX) alloy targets. From all targets, nanoparticles (sizes from 2 to 120 nm) can be produced in ethanol with a nearly equiatomic CoCrFeMnNi composition and a FCC structure, showing oxidation of up to 20 at.%. Nanoparticles from HEA‐PP exist in a solid solution state, while those from HEA‐BP and HEA‐SX form core‐shell structures with a Mn shell due to inhomogeneous material expulsion, confirmed by mass spectrometry. HEA‐PP PLAL synthesis demonstrates 6.8 % and 15.1 % higher productivity compared to HEA‐BP and HEA‐SX, establishing PLAL of elemental powder‐pressed targets as a reliable, time‐efficient, and cost‐effective method for generating solid solution HEA nanoparticles.
Operando Studies for CO2/CO Reduction in Flow‐Based Devices
Authors: Lin Zih‐Yi , Chang Yu‐Chia , Chen Yi‐Yu , Hsu Yung‐Hsi , Peng Kang‐Shun , Hung Sung‐Fu 
Published: March 2024

AbstractElectrocatalytic CO2 reduction reaction (CO2RR) conducted in a flow‐based device exhibits a substantial enhancement in Faradaic efficiency and catalytic current density compared to a conventional H‐type configuration. This highlights the distinct catalytic environment and behavior inherent in flow cells as opposed to H cells. To investigate the authentic properties of a catalyst within a flow‐based device, customized flow cells have been specifically devised for operando techniques during CO2RR and CORR, rather than resorting to an in‐situ three‐electrode H‐type configuration with its disparate catalytic environment and performance. This approach ensures a catalytic environment identical to that employed in electrochemical measurements. This review delineates the disparities between H‐type and flow‐based cells as well as the operando techniques tailored for flow‐based devices, including X‐ray absorption spectroscopy and Raman spectroscopy, preserving a consistent catalytic environment. It also compiles recent findings on copper‐based systems using operando flow‐based devices. The operando insights reveal a significant augmentation in catalytic current density, impacting both chemical properties and crystal structures. Furthermore, the observation of various catalytic intermediates enriches our comprehension. In essence, the application of operando techniques to flow‐based devices furnishes a comprehensive understanding of the catalytic behavior exhibited by diverse systems, propelling progress toward achieving Net Zero emissions.
The paradox of plant preference: The malaria vectors Anopheles gambiae and Anopheles coluzzii select suboptimal food sources for their survival and reproduction
Authors: Paré Prisca S. L. , Hien Domonbabele F. D. S. , Youba Mariam , Yerbanga Rakiswendé S. , Cohuet Anna , Gouagna Louis‐Clément , Diabaté Abdoulaye , Ignell Rickard , Dabiré Roch K. , Gnankiné Olivier , Lefèvre Thierry 
Published: March 2024

AbstractAnopheles gambiae and Anopheles coluzzii mosquitoes, two major malaria vectors in sub‐Saharan Africa, exhibit selectivity among plant species as potential food sources. However, it remains unclear if their preference aligns with optimal nutrient intake and survival. Following an extensive screening of the effects of 31 plant species on An. coluzzii in Burkina Faso, we selected three species for their contrasting effects on mosquito survival, namely Ixora coccinea, Caesalpinia pulcherrima, and Combretum indicum. We assessed the sugar content of these plants and their impact on mosquito fructose positivity, survival, and insemination rate, using Anopheles coluzzii and Anopheles gambiae, with glucose 5% and water as controls. Plants displayed varying sugar content and differentially affected the survival, sugar intake, and insemination rate of mosquitoes. All three plants were more attractive to mosquitoes than controls, with An. gambiae being more responsive than An. coluzzii. Notably, C. indicum was the most attractive but had the lowest sugar content and offered the lowest survival, insemination rate, and fructose positivity. Our findings unveil a performance–preference mismatch in An. coluzzii and An. gambiae regarding plant food sources. Several possible reasons for this negative correlation between performance and preference are discussed.
Theoretical Identification on Typical Fe3X (X=B, C, N) Compounds for Polysulfide Conversion in Lithium‐Sulfur Batteries
Authors: Sun Hongxu , Qian Chengfei , Guo Cong , Li Min , Tao Yibin , Li Jingfa 
Published: March 2024

AbstractLi−S batteries (LSBs) are considered as the attractive candidates for next‐generation high‐energy system due to their high energy and low cost. However, their practical application is hindered by several stubborn issues, including the poor electric conductivity of sulfur cathodes, the shuttle of lithium polysulfides (LiPSs) and the slow dynamics during charge/discharge cycles. Transitional iron (Fe)‐based compounds are regarded as effectively electrocatalysts for polysulfide conversion by accelerating the reaction kinetics and enhancing the electric conductivity and electron/charge transfer. In this study, we investigate the typical transition Fe‐based compounds (Fe3X, X=B, C, N), known for their high catalytic ability, analyze their roles as sulfur host for LSBs using density functional theory (DFT). Our finding reveals that Fe3C and Fe3B surfaces exhibit more Fe‐S bonds compared to Fe3N surface, which explains the different electrochemical behaviors observed during battery testing with sulfur cathodes. Additionally, Fe3N demonstrates greater structural stability and effective polysulfide adsorption according to DFT calculations, outperforming the other two compounds in these aspects. We believe that this theoretical investigation would guide the identification of highly efficient hosts for sulfur cathodes and open new avenues for sulfur host selection in LSBs.
Pain trajectories and neuropathic pain symptoms following lung cancer surgery: A prospective cohort study
Authors: Danielsen A. V. , Andreasen J. J. , Dinesen B. , Hansen J. , Petersen K. K. , Duch K. S. , Bisgaard J. , Simonsen C. , Arendt‐Nielsen L. 
Published: March 2024

AbstractBackgroundPersistent postsurgical pain (PPSP) after lung cancer surgery is common and current definitions are based on evaluations at a single time point after surgery. Pain intensity and symptoms may however fluctuate and change over time, and be impacted by multiple and shifting factors. Studies of postoperative recovery patterns and transition from acute to chronic pain are needed for further investigation of preventive measures and treatments to modify unfavourable recovery paths.MethodsIn this explorative study, 85 patients undergoing surgery due to either presumptive or confirmed lung cancer reported pain intensities bi‐monthly for 12 months. Pain trajectories during recovery were investigated, using group‐based trajectory modelling. Associations with possible risk factors for PPSP, including clinical variables and anxiety and depression score (HADS), were also explored.ResultsA trajectory model containing three 12‐month pain recovery groups was computed. One group without PPSP fully recovered (50%) within two to three months. Another group with mild‐intensity PPSP followed a protracted recovery trajectory (37%), while incomplete recovery was observed in the last group (13%). Acute postoperative pain and younger age were associated with a less favourable recovery trajectory. More neuropathic pain symptoms were observed in patients with incomplete recovery.ConclusionsThree clinically relevant recovery trajectories were identified, based on comprehensive pain tracking. Higher acute postoperative pain intensity was associated with an unfavourable pain recovery trajectory.Significance StatementUnderstanding the transition from acute to chronic postoperative pain and identifying preoperative risk factors is essential for the development of targeted treatments and the implementation of preventive measures. This study (1) identified distinct recovery trajectories based on frequent pain assessment follow‐ups for 12 months after surgery and (2) evaluated risk factors for unfavourable postoperative pain recovery paths. Findings suggest that early higher postoperative pain intensity is associated with an unfavourable long‐term recovery path.
Low‐density lipoprotein receptor‐related protein 5/6 promotes endometrial cancer progression and cancer cell immune escape
Authors: Shen Lifan , Zhang Chen , Zhu Genhai 
Published: March 2024

AbstractThe study investigated the potential association of the low‐density lipoprotein (LDL) genome with endometrial cancer progression based on the Gene Expression Omnibus data set and The Cancer Genome Atlas data set. Differential and weighted gene coexpression network analysis was performed on endometrial cancer transcriptome datasets GSE9750 and GSE106191. The protein‐protein interaction network was built using LDL‐receptor proteins and the top 50 tumor‐associated genes. Low‐density lipoprotein‐related receptors 5/6 (LRP5/6) in endometrial cancer tissues were correlated with oncogenes, cell cycle‐related genes, and immunological checkpoints using Spearman correlation. MethPrimer predicted the LRP5/6 promoter CpG island. LRP2, LRP6, LRP8, LRP12, low‐density lipoprotein receptor‐related protein‐associated protein, and LRP5 were major LDL‐receptor‐related genes associated with endometrial cancer. LRP5/6 was enriched in various cancer‐related pathways and may be a key LDL‐receptor‐related gene in cancer progression. LRP5/6 may be involved in the proliferation process of endometrial cancer cells by promoting the expression of cell cycle‐related genes. LRP5/6 may be involved in the proliferation of endometrial cancer cells by promoting the expression of cell cycle‐related genes. LRP5/6 may promote the immune escape of cancer cells by promoting the expression of immune checkpoints, promoting endometrial cancer progression. The MethPrimer database predicted that the LRP5/6 promoter region contained many CpG islands, suggesting that DNA methylation can occur in the LRP5/6 promoter region. LRP5/6 may aggravate endometrial cancer by activating the phosphoinositide 3‐kinase/protein kinase B pathway.
The mobile giving gap: The negative impact of smartphones on donation behavior
Authors: Hock Stefan J. , Ferguson Kristen A. , Herd Kelly B. 
Published: March 2024

AbstractWhile charities typically use the same messaging when appealing to consumers on their smartphones and PCs, this approach may backfire. Across three studies, we find consumers are less likely to donate on their smartphones (vs. PCs), a phenomenon we call the mobile giving gap. In study 1, we demonstrate that consumers are less willing to donate real money to a charitable organization. In study 2, we provide process support and demonstrate that the focal effect is mediated by other‐focus. Finally, a field experiment using Google display ads (study 3) replicates the focal effect and demonstrates that the negative impact of smartphones is attenuated when the appeal explicitly focuses on others (vs. the self). This study not only provides additional process support, but also suggests an easily implementable strategy that charities can use to close the mobile giving gap. Taken together, our findings offer theoretical insights related to the mobile mindset and its impact on consumer behavior and highlight that charities should tailor their donation appeals based on device type.
Single nucleotide polymorphisms of ANKK1, DDR4, and GRIN2B genes predict behavior in a prospective cohort of Mexican children and adolescents
Authors: Moctezuma Barbara , Santiago Ángel , Burguete‐García Ana , Martínez‐Barnetche Jesus , Morales‐Gómez Claudia , Hernandez‐Chavez Carmen , Gil Gabriela , Peterson Karen E. , Tellez‐Rojo Martha M. , Lamadrid‐Figueroa Hector 
Published: March 2024

AbstractNumerous studies have established associations between single nucleotide polymorphisms (SNPs) and various behavioral and neurodevelopmental conditions. This study explores the links between SNPs in candidate genes involved in central nervous system (CNS) physiology and their implications for the behavioral and emotional aspects in children and teenagers. A total of 590 participants, aged 7–15 years, from the Early Life Exposures In Mexico To Environmental Toxicants (ELEMENT) cohort study in Mexico City, underwent genotyping for at least one of 15 CNS gene‐related SNPs at different timepoints. We employed multiple linear regression models to assess the potential impact of genetic variations on behavioral and cognitive traits, as measured by the Behavioral Assessment System for Children (BASC) and Conners parent rating scales. Significant associations were observed, including the rs1800497 TC genotype (ANKK1) with the Cognitive Problems/Inattention variable (p value = 0.003), the rs1800955 CT genotype (DDR4) with the Emotional Lability Global index variable (p value = 0.01), and the rs10492138 GA and rs7970177 TC genotypes (GRIN2B) with the Depression variable (p values 0.007 and 0.012, respectively). These finds suggest potential genetic profiles associated with “risk” and “protective” behaviors for these SNPs. Our results provide valuable insights into the role of genetic variations in neurobehavior and highlight the need for further research in the early identification and intervention in individuals at risk for these conditions.
What is a trait? Lessons from the human chin
Authors: Meneganzin Andra , Ramsey Grant , DiFrisco James 
Published: March 2024

AbstractThe chin, a distinguishing feature of Homo sapiens, has sparked ongoing debates regarding its evolutionary origins and adaptive significance. We contend that these controversies stem from a fundamental disagreement about what constitutes a well‐defined biological trait, a problem that has received insufficient attention despite its recognized importance in biology. In this paper, we leverage paleoanthropological research on the human chin to investigate the general issue of character or trait identification. First, we examine four accounts of the human chin from the existing literature: the mandibular differential growth byproduct, the bony prominence, the inverted T‐relief, and the symphyseal angle. We then generalize from these accounts and propose a three‐stage framework for the process of character identification: description, detection, and justification. We use this framework to reinterpret the four accounts, elucidating key points of contention surrounding the chin as well as other morphological characters. We show that debates over the chin carry broad and important biological implications that extend beyond this trait and that are not mere semantic issues of definition.