bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Long Non-Coding RNAs in the Pathogenesis of Essential
Hypertension
Authors: Azizidoost Shirin , Khojasteh Pour Fatemeh , Najafi Sajad , Ghaedrahmati Farhoodeh , Nazeri Zahra , Dayer Dian , Shabani Zahra , Farzaneh Maryam , Nasrolahi Ava 
Published: March 2024

Abstract:
Hypertension, a condition characterized by elevated blood pressure, poses a significant medical risk, particularly in relation to the development of cardiovascular disease. The
pathogenesis of hypertension can be influenced by a combination of genetic and environmental
factors. Recent scientific studies have shed light on the involvement of long-non-coding RNAs
(lncRNAs) in various diseases, including pulmonary arterial and essential hypertension. These
lncRNAs have been found to play crucial roles at the transcriptional, post-transcriptional, and
post-translational levels. By targeting endothelial cell proliferation, migration, apoptosis, and
angiogenesis, lncRNAs can induce dysfunction in arterial endothelial cells, contributing to the
progression of hypertension-related pathological events. Therefore, the abnormal expression of
lncRNAs has emerged as a significant factor in the understanding of essential hypertension and
its associated mechanisms. To further our understanding, we conducted a comprehensive review
focusing on unraveling the functional roles of lncRNAs in essential hypertension.
Rapid In Vitro Regeneration and Genetic Fidelity Assessment of Regenerated Plants in Ayapana Triplinervis (Vahl) R.M. King & H. Robinson: An Ethnomedicinal and Ornamental Herb
Authors: Krishna Thumadath Palayullaparambil Ajeesh , Krishnakumar Neenthamadathil Mohandas , Maharajan Theivanayagam , Ceasar Stanislaus Antony 
Published: March 2024

Background:
Ayapana triplinervis is a popular ethnomedicinal and ornamental plant species. Due to its high medicinal importance, A. triplinervis was recently documented in the French Pharmacopeia.

Objective:
Rapid and efficient tissue culture protocol development is crucial for the high production and biotechnological applications of this plant.

Methods:
In this study, an efficient tissue culture protocol was developed for plant regeneration using nodal explants of A. triplinervis. The nodal explants were treated in Murashige and Skoog’s (MS) medium supplemented with various individual concentrations of cytokinins (BAP and KIN) and auxins (IAA and IBA). The nodal explant was regenerated in three different steps: (1) initial shoot induction, (2) shoot multiplication and elongation, and (3) rooting.

Results:
The results revealed that all individual concentrations (10, 20, 30, or 40 mg/L) of BAP or KIN responded to induce shoot initiation. The highest shoot multiplication and elongation were achieved in the MS medium with 20 mg/L BAP and 20 mg/L KIN. The regenerated plantlets produced better roots on MS medium containing 1.0 mg/L of each IAA or IBA. The well-established rooted plantlets were maintained in the culture room and greenhouse for better acclimatization and achieved a 100% survival rate. We analyzed the genetic fidelity of in vitro regenerated plants using random amplified polymorphic DNA (RAPD) markers. No genetic polymorphisms were observed in vitro plants compared to the mother plants.

Conclusion:
This efficient protocol could benefit future biotechnological applications like mass multiplication, genetic transformation and gene editing for improving the bioactive molecules in A. triplinervis.
Double Layer Sign and Pigment Epithelial Detachment in Patients Treated with Subthreshold Laser for Central Serous Chorioretinopathy
Authors: Enders Christian , Lang Gabriele E. , Mayer Benjamin , Werner Jens Ulrich 
Published: March 2024

Background and Objective
Central serous chorioretinopathy (CSCR) is characterized by serous detachments of the central neurosensory retina. Besides, other alterations of the retinal pigment epithelium (RPE) of the focal detachment of the RPE can occur. We explored the frequency of RPE detachment and double layer sign in OCT in our patient population, the influence of subthreshold photocoagulation (ST-LP) on these parameters and the possibility of correlations with visual acuity.

Materials and Methods
We conducted a retrospective analysis of all patients who underwent ST-LP based on the diagnosis of CSCR in a German university eye clinic from 2009 to 2014. Measurements in OCT were performed regarding pigment epithelial detachment (PED) and double layer sign (DLS).

Results
54 eyes of 49 patients were included in the study. The frequency of PEDs dropped from 66% of cases at baseline to 50% after 3 months. No significant differences in calculated areas of lift-off volume of PED were identified. The frequency of a DLS was significantly lower 8 weeks after ST-LP compared to baseline (63%, p<0.05), but not after 12 weeks.

Conclusion
We conclude that PEDs and DLS are common in CSCR. In this study, no convincing correlation can be identified between BCVA before ST-LP or at the last visit and different characteristics of PED as well as DLS. While the area of PED becomes smaller after ST-LP, the extent of the DLS seems to increase. Further prospective studies are required, and DLS with respect to the occurrence of CNV should also be given attention.
Industry 4.0 Technologies in Maternal Health Care: Bibliometric Analysis and Research Agenda
Authors: Sibanda Khulekani , Ndayizigamiye Patrick , Twinomurinzi Hossana 
Published: March 2024

Background
Industry 4.0 (I4.0) technologies have improved operations in health care facilities by optimizing processes, leading to efficient systems and tools to assist health care personnel and patients.

Objective
This study investigates the current implementation and impact of I4.0 technologies within maternal health care, explicitly focusing on transforming care processes, treatment methods, and automated pregnancy monitoring. Additionally, it conducts a thematic landscape mapping, offering a nuanced understanding of this emerging field. Building on this analysis, a future research agenda is proposed, highlighting critical areas for future investigations.

Methods
A bibliometric analysis of publications retrieved from the Scopus database was conducted to examine how the research into I4.0 technologies in maternal health care evolved from 1985 to 2022. A search strategy was used to screen the eligible publications using the abstract and full-text reading. The most productive and influential journals; authors’, institutions’, and countries’ influence on maternal health care; and current trends and thematic evolution were computed using the Bibliometrix R package (R Core Team).

Results
A total of 1003 unique papers in English were retrieved using the search string, and 136 papers were retained after the inclusion and exclusion criteria were implemented, covering 37 years from 1985 to 2022. The annual growth rate of publications was 9.53%, with 88.9% (n=121) of the publications observed in 2016-2022. In the thematic analysis, 4 clusters were identified—artificial neural networks, data mining, machine learning, and the Internet of Things. Artificial intelligence, deep learning, risk prediction, digital health, telemedicine, wearable devices, mobile health care, and cloud computing remained the dominant research themes in 2016-2022.

Conclusions
This bibliometric analysis reviews the state of the art in the evolution and structure of I4.0 technologies in maternal health care and how they may be used to optimize the operational processes. A conceptual framework with 4 performance factors—risk prediction, hospital care, health record management, and self-care—is suggested for process improvement. a research agenda is also proposed for governance, adoption, infrastructure, privacy, and security.
Emerging Trends of Self-Harm Using Sodium Nitrite in an Online Suicide Community: Observational Study Using Natural Language Processing Analysis
Authors: Das Sudeshna , Walker Drew , Rajwal Swati , Lakamana Sahithi , Sumner Steven A , Mack Karin A , Kaczkowski Wojciech , Sarker Abeed 
Published: March 2024

Abstract

Background
There is growing concern around the use of sodium nitrite (SN) as an emerging means of suicide, particularly among younger people. Given the limited information on the topic from traditional public health surveillance sources, we studied posts made to an online suicide discussion forum, “Sanctioned Suicide,” which is a primary source of information on the use and procurement of SN.

Objective
This study aims to determine the trends in SN purchase and use, as obtained via data mining from subscriber posts on the forum. We also aim to determine the substances and topics commonly co-occurring with SN, as well as the geographical distribution of users and sources of SN.

Methods
We collected all publicly available from the site’s inception in March 2018 to October 2022. Using data-driven methods, including natural language processing and machine learning, we analyzed the trends in SN mentions over time, including the locations of SN consumers and the sources from which SN is procured. We developed a transformer-based source and location classifier to determine the geographical distribution of the sources of SN.

Results
Posts pertaining to SN show a rise in popularity, and there were statistically significant correlations between real-life use of SN and suicidal intent when compared to data from the Centers for Disease Control and Prevention (CDC) Wide-Ranging Online Data for Epidemiologic Research (⍴=0.727; P<.001) and the National Poison Data System (⍴=0.866; P=.001). We observed frequent co-mentions of antiemetics, benzodiazepines, and acid regulators with SN. Our proposed machine learning–based source and location classifier can detect potential sources of SN with an accuracy of 72.92% and showed consumption in the United States and elsewhere.

Conclusions
Vital information about SN and other emerging mechanisms of suicide can be obtained from online forums.
Impact of Incentives on Physician Participation in Research Surveys: Randomized Experiment
Authors: Hawa Saadiya , Bane Shalmali , Kinsler Kayla , Rector Amadeia , Chaichian Yashaar , Falasinnu Titilola , Simard Julia F 
Published: March 2024

Background
Web-based surveys can be effective data collection instruments; however, participation is notoriously low, particularly among professionals such as physicians. Few studies have explored the impact of varying amounts of monetary incentives on survey completion.

Objective
This study aims to conduct a randomized study to assess how different incentive amounts influenced survey participation among neurologists in the United States.

Methods
We distributed a web-based survey using standardized email text to 21,753 individuals randomly divided into 5 equal groups (≈4351 per group). In phase 1, each group was assigned to receive either nothing or a gift card for US $10, $20, $50, or $75, which was noted in the email subject and text. After 4 reminders, phase 2 began and each remaining individual was offered a US $75 gift card to complete the survey. We calculated and compared the proportions who completed the survey by phase 1 arm, both before and after the incentive change, using a chi-square test. As a secondary outcome, we also looked at survey participation as opposed to completion.

Results
For the 20,820 emails delivered, 879 (4.2%) recipients completed the survey; of the 879 recipients, 622 (70.8%) were neurologists. Among the neurologists, most were male (412/622, 66.2%), White (430/622, 69.1%), non-Hispanic (592/622, 95.2%), graduates of American medical schools (465/622, 74.8%), and board certified (598/622, 96.1%). A total of 39.7% (247/622) completed their neurology residency more than 20 years ago, and 62.4% (388/622) practiced in an urban setting. For phase 1, the proportions of respondents completing the survey increased as the incentive amount increased (46/4185, 1.1%; 76/4165, 1.8%; 86/4160, 2.1%; 104/4162, 2.5%; and 119/4148, 2.9%, for US $0, $10, $20, $50, and $75, respectively; P<.001). In phase 2, the survey completion rate for the former US $0 arm increased to 3% (116/3928). Those originally offered US $10, $20, $50, and $75 who had not yet participated were less likely to participate compared with the former US $0 arm (116/3928, 3%; 90/3936, 2.3%; 80/3902, 2.1%; 88/3845, 2.3%; and 74/3878, 1.9%, for US $0, $10, $20, $50, and $75, respectively; P=.03). For our secondary outcome of survey participation, a trend similar to that of survey completion was observed in phase 1 (55/4185, 1.3%; 85/4165, 2%; 96/4160, 2.3%; 118/4162, 2.8%; and 135/4148, 3.3%, for US $0, $10, $20, $50, and $75, respectively; P<.001) and phase 2 (116/3928, 3%; 90/3936, 2.3%; 80/3902, 2.1%; 88/3845, 2.3%; and 86/3845, 2.2%, for US $0, $10, $20, $50, and $75, respectively; P=.10).

Conclusions
As expected, monetary incentives can boost physician survey participation and completion, with a positive correlation between the amount offered and participation.
INTENSI MENIKAH KEMBALI PADA SINGLE MOTHER
Authors: Naf’ah Ana Nihlatun 
Published: March 2024

Tujuan dari penelitian ini adalah untuk mendeskripsikan intensi menikah kembali pada single mother. Subjek pada penelitian ini adalah single mother sebanyak 77 subjek dengan rentang usia 21 – 85 tahun. Teknik pengambilan sampel menggunakan teknik sampel purposif. Penelitian ini menggunakan pendekatan kuantitatif deskriptif yang memakai skala intensi menikah kembali dengan model skala likert. Analisa datanya menggunakan perhitungan Z-score dengan maksud kategorisasi. Hasil penelitian ini menunjukkan 58% (45 subjek) memiliki intensi menikah kembali rendah, dan 42% (32 subjek) memiliki intensi menikah kembali tinggi. Dari ketiga aspek pembentuk intensi, hasil penelitiannya adalah behavior belief (42%), normative belief (28%) serta behavior control (30%). Pada intensi menikah kembali rendah, behavior control (52%), normative belief (51%) dan behavior belief (50%). Sedangkan pada intensi menikah kembali tinggi, behavior belief (50%), normative belief (49%) dan behavior control (48%). Kata kunci: Intensi menikah kembali, single mother This study aimed at describing single mothers’ intention of getting remarried. The subjects of this study were 77 single mothers aged 21 up to 85 years old. Using purposive sampling technique, this quantitative-descriptive study employed Likert-type getting-remarried intention scale. The data were then analyzed by calculating the z-score for categorization. The results of the study showed that 58% of the subjects (45 people) had low intention of getting remarried, while 42% of them (32 people) had high intention. Furthermore, three aspects varied in triggering the intention, they were behavior belief (42%), normative belief (28%) and behavior control (30%). For the low intention of getting remarried, the behavior control was 52% of significance, the normative belief was 51%, and the behavior belief was 50%. Meanwhile, for the high intention, the behavior belief was 50%, the normative belief was 49%, and the behavior control was 48%. Keyword: Intention of getting remarried, single mother
Analisis Strategi Pemasaran Dengan Menggunakan Metode SWOT Dan QSPM Dalam Upaya Meningkatkan Volume Penjualan (Studi Pada Endorfin Coffee Di Kecamatan Metro Timur)
Authors: Suwarto  , Suryadi  , Hexza Zahwa Novinka Putri  
Published: March 2024

Since the beginning of the product opening sale that the selling of Endorfin Coffee have continued to decline. Furthermore, many business players in the area offer similar products with their variations which later on contribute to the high level of sale competition in Metro town itself, such as the national ones like Starbucks to local ones like Janji Jiwa. Therefore, selling Endorfin Coffee requires an appropriate and effective marketing strategy.
This research was conducted by using a quantitative descriptive research method that aims to determine the marketing strategies applied to Endorphin Coffee in facing competition among the coffee shops around the area. (Sugiyono: 35) Quantitative research method is a research method that based on the philosophy of positivism is used to examine certain population and samples to test established hypotheses.
Endorfin Coffee sale goes in quadrant I position with a Grow and Built development strategy, therefore the appropriate strategies applied are Market Development, Product Development, and Market Penetration. In this position, the company indicates has a strong organization and the opportunity to recommend a progressive strategy, in which the organization is in good condition. This company should be able to see the external factors (opportunities and threats) that exist in the company to win the competition. The factors can be reached by conducting marketing effectiveness through involving the information technology in marketing and designing or making layout of the company product to make it more attractive to the costumers. The company can also penetrate the market not only for students, but also for all members of the society in and outside of the Metro town.
Penggunaan Website sebagai Strategi Online Fundraising di Yayasan Al-Qomariyah Kota Bandung
Authors: Dianto Nurul Izza Sayyidina Aufa , Irfan Maulana , Gutama Arie Surya 
Published: March 2024

Penelitian ini membahas penerapan strategi online fundraising oleh Yayasan Al-Qomariyah, Kota Bandung, dengan fokus pada pemanfaatan website company profile. Melalui pendekatan kualitatif, penelitian ini bertujuan untuk menggambarkan aspek-aspek yang memengaruhi kesuksesan penggalangan dana online, seperti aksesibilitas, akuntabilitas, edukasi, interaksi, dan pemberdayaan. Hasil penelitian menunjukkan bahwa Yayasan Al-Qomariyah telah memanfaatkan kombinasi pendekatan yang inklusif melalui online fundraising untuk mencapai keberlanjutan dalam dukungan finansial dan direct funding pada proyek spesifik. Website Al-Qomariyah telah mengakomodasi pengalaman berdonasi yang baik, tetapi interaksi lebih fokus dilakukan melalui media sosial. Transparansi penggunaan dana tidak disajikan secara eksplisit melalui website unuk menjaga privasi donatur. Interaksi dalam website masih belum optimal, sehingga media sosial dimanfaatkan untuk meningkatkan interaksi tersebut dan menjadi wadah utama dalam mendorong partisipasi dan donasi. Penelitian ini merekomendasikan Yayasan Al-Qomariyah untuk melakukan integrasi yang lebih baik antara website dengan platform penggalangan dana online lainnya, meningkatkan interaksi di website, serta memperbanyak konten edukatif untuk mendorong partisipasi aktif dalam peningkatan online fundraising mereka.
A propósito de “7 días en Entebbe”: una revisión jurídica de la Operación Thunderbolt
Authors: Obregón Fernández Aritz 
Published: March 2024

En este artículo sobre cine y Derecho internacional, atendemos a los hechos narrados en la película “7 días en Entebbe” (2018), del director brasileño José Padilha, a fin de redescubrir y analizar la argumentación jurídica de Israel para justificar la Operación Thunderbolt, destinada a rescatar a los rehenes secuestrados por parte de militantes del Frente Popular para la Liberación de Palestina y la organización alemana Revolutionäre Zellen. Concretamente, identificamos la invocación del derecho a la legítima defensa interpretado conforme a lo señalado en el caso Caroline, una proto-doctrina unwilling or unable, y otros argumentos menores, como el rescate de nacionales en el extranjero o la calificación del secuestro como acto de piratería.
Experience with the Ketogenic Diet in a Boy with CLCN4 Related Neurodevelopmental Disorder
Authors: Sager G , Yukselmis U , Güzel O , Turkyilmaz A , Akcay M 
Published: March 2024

Abstract
Raynaud-Claes syndrome is rare condition characterized with intellectual disability and is caused by X-linked pathogenic variants in CLCN4 gene. Hemizygous missense variant NM_001830.4: c.1597G>A (p.V533M) was detected in a 6-year-old male followed up with intellectual disability, dysmorphism, and epileptic encephalopathy. The mother and one sister of the patient were also carrying the same variant. The clinical picture of the patient was significantly more severe, and the patient exhibited nonconvulsive status. Tonic status was observed with benzodiazepine treatment and the patient was successfully treated with a ketogenic diet. Many types of seizures can be seen in Raynaud-Claes syndrome, some of which can be life-threatening. CLCN4 variants can be investigated in patients who exhibit an increase in tonic seizures with benzodiazepine treatment. However, ketogenic dietary therapy as first-line treatment can be lifesaving in resistant epilepsy cases caused by the CLCN4 gene.
Chromosomal Abnormalities in Early Pregnancy Losses: A Study of 900 Samples
Authors: Bozhinovski Gj , Terzikj M , Kubelka-Sabit K , Jasar Dz , Lazarevski S , Livrinova V , Plaseska-Karanfilska D 
Published: March 2024

Abstract
Chromosomal abnormalities are the most common causes of early pregnancy losses (EPLs). In this study, we aimed to evaluate the incidence and spectrum of chromosomal abnormalities in EPLs and correlate them with different clinical characteristics. We performed Quantitative Fluorescent PCR (QF-PCR), followed by subtelomeric Multiplex Ligation Probe Amplification (MLPA) analysis to detect chromosomal abnormalities in 900 products of conceptions (POCs) from EPLs collected over a period of 10 years.
Chromosomal abnormalities were present in 56.25% of uncontaminated EPLs, with significantly higher incidence in women ≥36 years (71.37%, p<0.0001) in comparison to women ≤30 years of age (43.40%). Trisomies were also more common in women ≥36 years (79.68%, p<0.0001) than in those ≤30 years of age (48.70%). In contrast, triploidy and monosomies were more prevalent in women ≤30 years of age (26.09%, p<0.0001 and 16.52%, p=0.0066 respectively) than in women ≥36 years of age (6.42% and 6.42% respectively). Trisomy 16 was more common in women ≤30 (39.29%, p=0.0009) than in those ≥36 years of age (16.78%), while trisomy 22 was predominant among women ≥36 (23.49%, p=0.013), and was not present in the group of women ≤30 years of age. The frequency of chromosomal abnormalities in POCs from women with sporadic (61.19%) was higher than in those with recurrent EPLs (55.21%). This difference, however, was not statistically significant (p=0.164). Although some differences in the chromosomal aneuploidy rates among women with different ABO blood groups, as well as among 6–8 and 9–11 gestational week EPLs were observed, further larger studies are required to confirm these findings.
In conclusion, our study enriches the knowledge about chromosomal abnormalities as a cause of EPLs and confirms the higher incidence of foetal chromosomal abnormalities in EPLs in women of older reproductive age. Furthermore, it shows that using QF-PCR and MLPA methodologies, a high detection rate of chromosomal abnormalities in EPLs can be reached.
Severe Form of Salih Myopathy Caused by Combination of Two Heterozygous TTN Mutations
Authors: Milojković M , Jarić M , Stojanović V , Barišić N , Kavečan I 
Published: March 2024

Abstract
Salih myopathy is autosomal recessive hereditary early-onset myopathy with fatal cardiomyopathy. It is a rare and heterogeneous form of congenital titinopathies (TTN). Affected children have delayed motor development, normal mental development, and in further course dilated cardiomyopathy. Motor functions have a tendency to improve, but death occurs most often before 20 years of age due to arrhythmias.
Our patient is a 2-year-old girl, born in severe perinatal asphyxia, with global hypotonia and poor spontaneous movements. She required immediate endotracheal intubation and mechanical ventilation was initiated without the possibility of cessation. Improvement in her neurological status was not observed. Due to her clinical presentation, we performed genetic testing and a diagnosis of Salih myopathy caused by combination of two heterozygous TTN mutations was confirmed.
This case illustrates that Salih myopathy may have severe presentation from birth, with continuous necessity for mechanical ventilation, without any motor improvement.