bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacological Properties of DOV 315,090, an ocinaplon metabolite
Authors: Berezhnoy Dmytro , Gravielle Maria C , Downing Scott , Kostakis Emmanuel , Basile Anthony S , Skolnick Phil , Gibbs Terrell T , Farb David H 
Published: September 2008

AbstractBackgroundCompounds targeting the benzodiazepine binding site of the GABAA-R are widely prescribed for the treatment of anxiety disorders, epilepsy, and insomnia as well as for pre-anesthetic sedation and muscle relaxation. It has been hypothesized that these various pharmacological effects are mediated by different GABAA-R subtypes. If this hypothesis is correct, then it may be possible to develop compounds targeting particular GABAA-R subtypes as, for example, selective anxiolytics with a diminished side effect profile. The pyrazolo[1,5-a]-pyrimidine ocinaplon is anxioselective in both preclinical studies and in patients with generalized anxiety disorder, but does not exhibit the selectivity between α1/α2-containing receptors for an anxioselective that is predicted by studies using transgenic mice.ResultsWe hypothesized that the pharmacological properties of ocinaplonin vivomight be influenced by an active biotransformation product with greater selectivity for the α2subunit relative to α1. One hour after administration of ocinaplon, the plasma concentration of its primary biotransformation product, DOV 315,090, is 38% of the parent compound. The pharmacological properties of DOV 315,090 were assessed using radioligand binding studies and two-electrode voltage clamp electrophysiology. We report that DOV 315,090 possesses modulatory activity at GABAA-Rs, but that its selectivity profile is similar to that of ocinaplon.ConclusionThese findings imply that DOV 315,090 could contribute to the action of ocinaplon in vivo, but that the anxioselective properties of ocinaplon cannot be readily explained by a subtype selective effect/action of DOV 315,090. Further inquiry is required to identify the extent to which different subtypes are involved in the anxiolytic and other pharmacological effects of GABAA-R modulators.
Var2CSA DBL6-epsilon domain expressed in HEK293 induces limited cross-reactive and blocking antibodies to CSA binding parasites
Authors: Fernandez Pablo , Viebig Nicola K , Dechavanne Sébastien , Lépolard Catherine , Gysin Jürg , Scherf Artur , Gamain Benoit 
Published: September 2008

Abstract

Background
Pregnancy-associated malaria (PAM) is a serious consequence of Plasmodium falciparum-infected erythrocytes sequestration in the placenta through the adhesion to the placental receptor chondroitin sulfate A (CSA). Although women become resistant to PAM as they acquire transcending inhibitory immunity against CSA-binding parasites, hundreds of thousands of lives could be saved if a prophylactic vaccine targeting the surface proteins of placental parasites could be designed. Recent works point to the variant protein var2CSA as the key target for the development of a pregnancy-associated malaria vaccine. However, designing such a prophylactic vaccine has been hindered by the difficulty in identifying regions of var2CSA that could elicit broadly neutralizing and adhesion-blocking antibodies.

Methods
Var2CSA is a very large protein with an estimated molecular weight of 350 kDa, and can be divided into six cysteine rich Duffy binding-like domains (DBL). The human embryonic kidney 293 cell line (HEK293) was used to produce secreted soluble recombinant forms of var2CSA DBL domains. The Escherichia coli expression system was also assessed for the domains not expressed or expressed in low amount in the HEK293 system. To investigate whether var2CSA binding DBL domains can induce biologically active antibodies recognizing the native var2CSA and blocking the interaction, mice were immunized with the refolded DBL3-X or the HEK293 secreted DBL6-ε domains.

Results
Using the HEK293 expression system, DBL1-X, DBL4-ε and DBL6-ε were produced at relatively high levels in the culture supernatant, while DBL3-X and DBL5-ε were produced at much lower levels. DBL2-X and DBL3-X domains were obtained after refolding of the inclusion bodies produced in E. coli. Importantly, mice antisera raised against the recombinant DBL6-ε domain, specifically reacted against the surface of CSA-binding parasites and revealed adhesion blocking activity.

Conclusion
This is the first report showing inhibitory binding antibodies obtained through a var2CSA recombinant DBL domain immunization protocol. These results support the current strategies using var2CSA as immunogen in the aim of blocking placental sequestration of malaria parasites. This work is a step towards the development of a var2CSA based vaccine that will prevent pregnancy-associated malaria and improve pregnancy outcomes.
Early transplantation of human immature dental pulp stem cells from baby teeth to golden retriever muscular dystrophy (GRMD) dogs: Local or systemic?
Authors: Kerkis Irina , Ambrosio Carlos E , Kerkis Alexandre , Martins Daniele S , Zucconi Eder , Fonseca Simone AS , Cabral Rosa M , Maranduba Carlos MC , Gaiad Thais P , Morini Adriana C , Vieira Natassia M , Brolio Marina P , Sant'Anna Osvaldo A , Miglino Maria A , Zatz Mayana 
Published: September 2008

AbstractBackgroundThe golden retriever muscular dystrophy (GRMD) dogs represent the best available animal model for therapeutic trials aiming at the future treatment of human Duchenne muscular dystrophy (DMD). We have obtained a rare litter of six GRMD dogs (3 males and 3 females) born from an affected male and a carrier female which were submitted to a therapeutic trial with adult human stem cells to investigate their capacity to engraft into dogs muscles by local as compared to systemic injection without any immunosuppression.MethodsHuman Immature Dental Pulp Stem Cells (hIDPSC) were transplanted into 4 littermate dogs aged 28 to 40 days by either arterial or muscular injections. Two non-injected dogs were kept as controls. Clinical translation effects were analyzed since immune reactions by blood exams and physical scores capacity of each dog. Samples from biopsies were checked by immunohistochemistry (dystrophin markers) and FISH for human probes.Results and DiscussionWe analyzed the cells' ability in respect to migrate, engraftment, and myogenic potential, and the expression of human dystrophin in affected muscles. Additionally, the efficiency of single and consecutive early transplantation was compared. Chimeric muscle fibers were detected by immunofluorescence and fluorescentin situhybridisation (FISH) using human antibodies and X and Y DNA probes. No signs of immune rejection were observed and these results suggested that hIDPSC cell transplantation may be done without immunosuppression. We showed that hIDPSC presented significant engraftment in GRMD dog muscles, although human dystrophin expression was modest and limited to several muscle fibers. Better clinical condition was also observed in the dog, which received monthly arterial injections and is still clinically stable at 25 months of age.ConclusionOur data suggested that systemic multiple deliveries seemed more effective than local injections. These findings open important avenues for further researches.
A divergent role for estrogen receptor-beta in node-positive and node-negative breast cancer classified according to molecular subtypes: an observational prospective study
Authors: Novelli Flavia , Milella Michele , Melucci Elisa , Di Benedetto Anna , Sperduti Isabella , Perrone-Donnorso Raffaele , Perracchio Letizia , Venturo Irene , Nisticò Cecilia , Fabi Alessandra , Buglioni Simonetta , Natali Pier Giorgio , Mottolese Marcella 
Published: September 2008

Abstract
Introduction
Estrogen receptor-alpha (ER-α) and progesterone receptor (PgR) are consolidated predictors of response to hormonal therapy (HT). In contrast, little information regarding the role of estrogen receptor-beta (ER-β) in various breast cancer risk groups treated with different therapeutic regimens is available. In particular, there are no data concerning ER-β distribution within the novel molecular breast cancer subtypes luminal A (LA) and luminal B (LB), HER2 (HS), and triple-negative (TN).

Methods
We conducted an observational prospective study using immunohistochemistry to evaluate ER-β expression in 936 breast carcinomas. Associations with conventional biopathological factors and with molecular subtypes were analyzed by multiple correspondence analysis (MCA), while univariate and multivariate Cox regression analysis and classification and regression tree analysis were applied to determine the impact of ER-β on disease-free survival in the 728 patients with complete follow-up data.

Results
ER-β evenly distributes (55.5%) across the four molecular breast cancer subtypes, confirming the lack of correlation between ER-β and classical prognosticators. However, the relationships among the biopathological factors, analyzed by MCA, showed that ER-β positivity is located in the quadrant containing more aggressive phenotypes such as HER2 and TN or ER-α/PgR/Bcl2- tumors. Kaplan-Meier curves and Cox regression analysis identified ER-β as a significant discriminating factor for disease-free survival both in the node-negative LA (P = 0.02) subgroup, where it is predictive of response to HT, and in the node-positive LB (P = 0.04) group, where, in association with PgR negativity, it conveys a higher risk of relapse.

Conclusion
Our data indicated that, in contrast to node-negative patients, in node-positive breast cancer patients, ER-β positivity appears to be a biomarker related to a more aggressive clinical course. In this context, further investigations are necessary to better assess the role of the different ER-β isophorms.
Performance of an Energy-Conserving Algorithm for Multi-Body Dynamics
Authors: Dopico D , Naya M A , Lugris U , Cuadrado J 
Published: September 2008

This work presents the application to the dynamics of multibody systems of three methods based on the augmented Lagrangian techniques, compares them, and gives some criteria for their use in realistic problems. The methods are: an augmented Lagrangian formulation with trapezoidal rule plus projections of velocities and accelerations, an augmented Lagrangian energy-conserving formulation, and an augmented Lagrangian formulation with conserving integrator plus projections of velocities and accelerations. The simulation of two mechanical systems is carried out in this work: a spherical compound pendulum, which is an academic example that permits to see the properties of the formulations; andthe whole model of a car, which is a realistic and demanding example to test the efficiency and robustness of the formulations.
Why are Offices for Sale in China? A Case Study of the Office-Selling Chain in Heilongjiang Province
Authors: Zhu Jiangnan 
Published: September 2008

Abstract
This article analyzes how and why buying and selling offices (BSO) has re-emerged in China since the 1990s, from the perspective of demand and supply in a case study. The major causes are the weaknesses of the Chinese cadre management systems—concentration of power over personnel issues and the difficulties of monitoring top administrators.
Japan's Postal Privatization Battle: The Continuing Reverberations for the Liberal Democratic Party of Rebels-Assassins Conflicts
Authors: Carlson Matthew 
Published: September 2008

Abstract
This article argues that the use of campaign finance regulations and electoral rules by political parties significantly shaped the results of the 2005 general election and the battle over postal privatization in Japan. How the Liberal Democratic Party responds to the reverberations of the conflict between the so-called “rebels” and “assassins” is likely to affect its electoral fortunes in the next lower house election.
Flexible Employment in Hong Kong: Trends and Patterns in Comparative Perspective
Authors: Chiu Stephen W. K. , So Alvin Y. , Tam May Yeuk-mui 
Published: September 2008

Abstract
Through analyzing primary and secondary data, this paper argues that flexible employment practices in Hong Kong are largely employer-driven. This feature is explicable by the low level of government intervention in industrial relations, the development of the labor movement, and the Asian financial crisis that accentuated employers' prerogatives in employment relations.
Taiwan's Arms Procurement Debate and the Demise of the Special Budget Proposal: Domestic Politics in Command
Authors: Chase Michael S. 
Published: September 2008

Abstract
Despite the growing security threat posed by Chinese military modernization, Taiwan still has not completed the purchase of some of the key components of the arms sales package the United States approved in April 2001. The reasons include overconfidence in U.S. security assurances, underestimation of Chinese capabilities and resolve, and highly divisive domestic Taiwanese politics.
Soil ingestion may be an important route for the uptake of contaminants by some reptiles
Authors: Rich C. Nelson , Talent Larry G. 
Published: September 2008

Abstract
Some species of reptiles regularly ingest soil in the wild. Therefore, we evaluated the importance of soil ingestion as a route for the uptake of contaminants in lizards. We used sand as a substitute for soil during the present study. Different groups of leopard geckos (Eublepharis macularius) were provided with a control and five sodium selenite–spiked sand mixtures during a 28-d study. Twenty lizards were assigned to a control group and to each of five selenium-spiked sand mixtures that consisted of nominal selenium (Se) concentrations of 0.05, 0.46, 4.57, 11.41, and 22.83 mg Se/kg dry sand. Leopard geckos readily ingested the Se-spiked sand. We observed concentration-related effects in several endpoints. Overall growth in body mass was the most sensitive endpoint and was significantly (p &lt; 0.05) lower in lizards that ingested the 4.57, 11.41, and 22.83 mg Se/kg sand mixtures compared to controls. Growth in snout-vent length, mean daily food ingestion, and food conversion efficiency were less sensitive and were significantly (p &lt; 0.05) lower in lizards that ingested the 11.41 and 22.83 mg Se/kg sand mixtures compared to controls. Although our results are based on nominal amounts of Se ingested, leopard geckos appear to be as sensitive to sodium selenite as birds and mammals. The present study suggests that ingestion of soil could be an important potential route for the uptake of soil contaminants in some reptiles and should be evaluated in ecotoxicological studies and risk analyses of reptiles.
An adult and pediatric size‐based contrast administration reduction phantom study for single and dual‐energy CT through preservation of contrast‐to‐noise ratio
Authors: Wang Jia , Duan Xinhui , Mahmood Usman , McKenney Sarah Eva , Brady Samuel Loren 
Published: April 2024

AbstractBackgroundGlobal shortages of iodinated contrast media (ICM) during COVID‐19 pandemic forced the imaging community to use ICM more strategically in CT exams.PurposeThe purpose of this work is to provide a quantitative framework for preserving iodine CNR while reducing ICM dosage by either lowering kV in single‐energy CT (SECT) or using lower energy virtual monochromatic images (VMI) from dual‐energy CT (DECT) in a phantom study.Materials and MethodsIn SECT study, phantoms with effective diameters of 9.7, 15.9, 21.1, and 28.5 cm were scanned on SECT scanners of two different manufacturers at a range of tube voltages. Statistical based iterative reconstruction and deep learning reconstruction were used. In DECT study, phantoms with effective diameters of 20, 29.5, 34.6, and 39.7 cm were scanned on DECT scanners from three different manufacturers. VMIs were created from 40 to 140 keV. ICM reduction by lowering kV levels for SECT or switching from SECT to DECT was calculated based on the linear relationship between iodine CNR and its concentration under different scanning conditions.ResultsOn SECT scanner A, while matching CNR at 120 kV, ICM reductions of 21%, 58%, and 72% were achieved at 100, 80, and 70 kV, respectively. On SECT scanner B, 27% and 80% ICM reduction was obtained at 80 and 100 kV. On the Fast‐kV switch DECT, with CNR matched at 120 kV, ICM reductions were 35%, 30%, 23%, and 15% with VMIs at 40, 50, 60, and 68 keV, respectively. On the dual‐source DECT, ICM reductions were 52%, 48%, 42%, 33%, and 22% with VMIs at 40, 50, 60, 70, and 80 keV. On the dual‐layer DECT, ICM reductions were 74%, 62%, 45%, and 22% with VMIs at 40, 50, 60, and 70 keV.ConclusionsOur work provided a quantitative baseline for other institutions to further optimize their scanning protocols to reduce the use of ICM.
Polymer‐Regulating MXene@Dopamine Electroactive Gel‐Inks for Textile‐Based Multi‐Protective Wearables
Authors: Yan Biaobiao , Liu Siqi , Yuan Yun , Hou Xunan , Zhou Man , Yu Yuanyuan , Wang Qiang , He Chaobin , Wang Ping 
Published: April 2024

Abstract2D transition metal carbide/nitride (MXene) show significant potential for fabricating flexible wearables due to its outstanding electroactive characteristic. However, the complex processes in rheology regulation and easy agglomeration of MXene nanosheets hinder their applications as inks for homogeneous printing and coating. Herein, an electroactive gel‐ink with a low concentration of MXene (20 mg mL−1) using poly(3,4‐ethylenedioxythiophene):poly(sodium 4‐styrenesulfonate) (PEDOT:PSS) as a dopant and conductive binder is developed. The dopamine‐involved modification and PEDOT:PSS doping together promote the formation of ordered lamellar structure of MXene nanosheets, and in turn the nanosheets can regulate the interconnected electronic structure of PEDOT:PSS, which enables the transition from micellar to linear structures. Through adjusting the combination ratio of dopamine‐modified MXene (MD) to PEDOT:PSS, the viscosity of MXene inks (MDP) is tunable within 1–104 Pa·s to realize scalable printing and other processing. Screen‐printing with MDP gel‐ink endows textiles with excellent conductive stability while retaining the inherent wearability of the original fabric. With high conductivity (109.6 S m−1) and low mid‐infrared emissivity (0.34), the decorated textiles exhibit remarkable multi‐protective abilities. This work provides a novel strategy for formulating versatile MXene inks that will facilitate the large‐scale fabrication of high‐performance personal wearable multi‐protective textiles.
Electrochemical Exfoliation and Growth of Nickel–Cobalt Layered Double Hydroxides@Black Phosphorus Hetero‐Nanostructure Textiles for Robust Foldable Supercapacitors
Authors: Zheng Yun , Zhang Yang , Man Zengming , Chen Wenxing , Lu Wangyang , Wu Guan 
Published: April 2024

AbstractAdvanced innovation of flexible electrode with adequate redox activity and stably mechanical endurance that promotes charges kinetic migration and faradaic storage is pivotal for textile‐based supercapacitors (T‐SCs). Herein, this study reports a high‐performance T‐SCs electrode based on nickel–cobalt layered double hydroxides@black phosphorus (NiCo‐LDHs@BP) on a conductive silk (c‐silk) textile (NiCo‐LDHs@BP/c‐silk). Under negative voltage‐induced electrochemical exfoliation, the Ni2+ and Co2+ are embedded into bulk BP framework to form exfoliated BP nanosheets, and the NiCo‐LDHs are in situ grown within the BP networks, generating 3D NiCo‐LDHs@BP hetero‐nanostructure. Significantly, the NiCo‐LDHs@BP exhibits a large space‐charge area, enhanced adsorption energy for OH− and accelerated charges transfer/storage as confirmed using density functional theory calculations. Additionally, the T‐SCs electrode is fabricated by loading the blended NiCo‐LDHs@BP, sericin, and carbon nanotubes on fibroin textile via a silk reconstruction strategy, producing large area production, superior mechanical flexibility, and impressive electrochemical performance. The resultant NiCo‐LDHs@BP/c‐silk electrode exhibits large specific capacitance of 1291.3 F g−1 and considerable rate capacity in 1 M KOH electrolyte. Furthermore, the flexible solid‐state asymmetric T‐SCs deliver high specific areal energy density of 279.6 µWh cm−2 and robust folding capability (85.6% capacitance retention after 5000 folding cycles), which successfully power wearable watch and heart rate meter devices.
Combination of Efficient Red Fluorescence and High Photothermal Conversion in the Second Near‐Infrared Window from Carbon Dots through Photoinduced Sodium‐Doping Approach
Authors: Wang Qingcheng , Zhang Tesen , Cheng Quansheng , Wang Bingzhe , Liu Yupeng , Xing Guichuan , Tang Zikang , Qu Songnan 
Published: April 2024

AbstractCarbon dots (CDs) are highly desired in biological applications. However, achieving a balance between photoluminescence (PL) efficiency and photothermal conversion efficiency (PTCE) is challenging. In this study, an unprecedent combination of efficient red fluorescence and high PTCE in the second near‐infrared (NIR‐II) window in a sodium‐doped CDs system is reported. Upon 808 nm laser irradiation, photo‐induced oxidation–reduction reactions happened on the surface of sodium cation‐functionalized CDs (Na‐CDs), leading to the partial reduction of surface‐functionalized sodium (Na) ions. The photo‐reduced Na atoms coordinated with sp2 C domains in the core, resulting in Na‐doped CDs (ir‐Na‐CDs) with an enhanced absorption band in the NIR‐II window and a high PTCE of 43% under 1064 nm laser irradiation (1 W cm−2). Composing the ir‐Na‐CDs with bovine serum albumin (BSA) enhanced the PLQY of the red emission to 31% in water without diminishing the PTCE in the NIR‐II region. Transient absorption spectra revealed that no energy transfer occurred between the red emission center and the NIR‐II absorption band, revealing a novel CDs system with independent Janus photophysical processes. Moreover, ir‐Na‐CDs@BSA exhibited negligible to low cytotoxicity and demonstrated tumor accumulation capacity after intravenous injection, enabling effective tumor photothermal therapy in the NIR‐II region.
Nonflammable Succinonitrile‐Based Deep Eutectic Electrolyte for Intrinsically Safe High‐Voltage Sodium‐Ion Batteries
Authors: Chen Jian , Yang Zhuo , Xu Xu , Qiao Yun , Zhou Zhiming , Hao Zhiqiang , Chen Xiaomin , Liu Yang , Wu Xingqiao , Zhou Xunzhu , Li Lin , Chou Shu‐Lei 
Published: April 2024

AbstractIntrinsically safe sodium‐ion batteries are considered as a promising candidate for large‐scale energy storage systems. However, the high flammability of conventional electrolytes may pose serious safety threats and even explosions. Herein, a strategy of constructing a deep eutectic electrolyte is proposed to boost the safety and electrochemical performance of succinonitrile (SN)‐based electrolyte. The strong hydrogen bond between S═O of 1,3,2‐dioxathiolane‐2,2‐dioxide (DTD) and the α‐H of SN endows the enhanced safety and compatibility of SN with Lewis bases. Meanwhile, the DTD participates in the inner Na+ sheath and weakens the coordination number of SN. The unique solvation configuration promotes the formation of robust gradient inorganic‐rich electrode–electrolyte interphase, and merits stable cycling of half‐cells in a wide temperature range, with a capacity retention of 82.8% after 800 cycles (25 °C) and 86.3% after 100 cycles (60 °C). Correspondingly, the full cells deliver tremendous improvement in cycling stability and rate performance.