bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics and influencing factors of anticipated HIV stigma among HIV‐negative/unknown MSM in China: A regression mixture model
Authors: Dai Zhenwei , Wu Yijin , Liu Xin , Fu Jiaqi , Si Mingyu , Chen Xu , Wang Hao , Xiao Weijun , Huang Yiman , Yu Fei , Mi Guodong , Su Xiaoyou 
Published: March 2024

AbstractBackgroundAnticipated HIV stigma among men who have sex with men's (MSM) has a severe negative effect on their physical and mental health wellbeing and hence requires specific attention. The current study aims to identify the characteristics and the psychosocial influencing factors of anticipated HIV stigma in MSM using regression mixture model (RMM) and to determine the cut‐off point of the seven‐item Anticipated HIV Stigma Questionnaire (AHSQ) using the receiver operating characteristic (ROC) analysis.MethodsA cross‐sectional study was conducted among HIV‐negative/unknown MSM from Blued online platform in China from December 16th, 2020 to March 1st, 2021, enrolling 1394 participants. Data were collected on demographic characteristics, perceived social support, anticipated HIV stigma, depressive symptoms, and HIV knowledge. Latent profile analysis was performed to identify different profiles of anticipated HIV stigma level. Chi‐square test, analysis of variance, and RMM analysis were conducted to explore the influencing factors in different profiles. ROC analyses were carried out to identify the cut‐off value of anticipated stigma.ResultsAmong the participants, three profiles of anticipated stigma were identified: “low anticipated HIV stigma” (12.0%), “moderate anticipated HIV stigma” (52.1%), and “severe anticipated HIV stigma” (35.9%). RMM analysis showed that higher income and higher levels of knowledge were positively associated with moderate anticipated HIV stigma, whereas full‐time job and social support were negatively associated with moderate anticipated HIV stigma; higher income, depressive symptoms, and knowledge were positively associated with severe anticipated HIV stigma, whereas minor ethnicity and social support were negatively associated with severe anticipated HIV stigma. ROC curve of the AHSQ showed that the optimal cut‐off value of ≥16 could indicate positive anticipated HIV stigma.ConclusionThe study focuses on the level of anticipated HIV stigma and its psycho‐socio influencing factors among HIV‐negative/unknown MSM. It provides evidence for implementing relevant psychological interventions to HIV‐negative/unknown MSM.
Nucleic acid vaccines‐based therapy for triple‐negative breast cancer: A new paradigm in tumor immunotherapy arena
Authors: Saleh Raed Obaid , Ibrahim Fatma M. , Pallathadka Harikumar , Kaur Irwanjot , Ahmad Irfan , Ali Saad Hayif Jasim , Redhee Ahmed Huseen , Ghildiyal Pallavi , Jawad Mohammed Abed , Alsaadi Salim B. 
Published: March 2024

AbstractNucleic acid vaccines (NAVs) have the potential to be economical, safe, and efficacious. Furthermore, just the chosen antigen in the pathogen is the target of the immune responses brought on by NAVs. Triple‐negative breast cancer (TNBC) treatment shows great promise for nucleic acid‐based vaccines, such as DNA (as plasmids) and RNA (as messenger RNA [mRNA]). Moreover, cancer vaccines offer a compelling approach that can elicit targeted and long‐lasting immune responses against tumor antigens. Bacterial plasmids that encode antigens and immunostimulatory molecules serve as the foundation for DNA vaccines. In the 1990s, plasmid DNA encoding the influenza A nucleoprotein triggered a protective and targeted cytotoxic T lymphocyte (CTL) response, marking the first instance of DNA vaccine‐mediated immunity. Similarly, in vitro transcribed mRNA was first successfully used in animals in 1990. At that point, mice were given an injection of the gene encoding the mRNA sequence, and the researchers saw the production of a protein. We begin this review by summarizing our existing knowledge of NAVs. Next, we addressed NAV delivery, emphasizing the need to increase efficacy in TNBC.
Cognitive flexibility and adaptive performance among workers: Testing the moderating effect of the situational strength on this relationship
Authors: Ouellette Samuel , Longato Sabrina , Mayer William , Bouchard Frédérique , Boudrias Jean‐Sébastien 
Published: March 2024

AbstractAdaptive performance (AP) is crucial for individuals to respond to changes in their work role. Cognitive flexibility (CF) plays a role in AP, but the impact of the work environment on this relationship is not clear. Situational strength theory suggests that a strong situation, characterized by clarity, consistency, constraints, and consequences, weakens the effect of individual characteristics on behavior. This study aimed to examine if situational strength moderates the CF‐AP relationship. A sample of 464 workers were recruited. Overall, the research hypothesis was not supported, indicating no moderation effect of situational strength. However, exploratory analysis suggests that constraints limit the contribution of CF to AP. Organizations should reduce constraints to leverage workers' CF for adaptive behaviors.
Interdialytic home systolic blood pressure variability increases all‐cause mortality in hemodialysis patients
Authors: Dong Liping , Tian Ming , Li Hua , Dong Junwu , Song Xiaohong 
Published: March 2024

AbstractBackgroundThe association between Interdialytic home blood pressure variability (BPV) and the prognosis of patients undergoing maintenance hemodialysis (MHD) largely unknown.HypothesisWe proposed the hypothesis that interdialytic home BPV exert effect on cardiac and all‐cause mortality among individuals undergoing MHD.MethodsA total of 158 patients receiving MHD at the hemodialysis unit of Wuhan Fourth Hospital between December 2019 and August 2020 were included in this prospective cohort study. Patients were divided into tertiles according to the systolic BPV (SBPV), and the primary endpoints were cardiac and all‐cause death. Kaplan–Meier analysis was used to assess the relationship between long‐term survival and interdialytic home SBPV. In addition, Cox proportional hazards regression models were used to identify risk factors contributing to poor prognosis.ResultsThe risk of cardiac death and all‐cause death was gradually increased in patients according to tertiles of SBPV (3.5% vs. 14.8% vs. 19.2%, p for trend = .021; and 11.5% vs. 27.8% vs. 44.2%, p for trend <.001). The Cox regression analysis revealed that compared to Tertile 1, the hazard ratios for all‐cause mortality in Tertile 2 and Tertile 3 were 3.13 (p = .026) and 3.24 (p = .021), respectively, after adjustment for a series of covariates.ConclusionsThe findings revealed a positive correlation between increased interdialytic home SBPV and elevated mortality risk in patients with MHD.
Symptomatic androgen deficiency and sexual dysfunctions in male patients receiving alectinib for ALK‐positive advanced non–small cell lung cancer
Authors: Vita Emanuele , Monaca Federico , Milardi Domenico , Mastrantoni Luca , Stefani Alessio , Vergani Edoardo , Russo Jacopo , Barone Diletta , Sparagna Ileana , Vitale Antonio , Scala Alessandro , Occhipinti Denis , Di Salvatore Mariantonietta , Pontecorvi Alfredo , Tortora Giampaolo , Bria Emilio 
Published: March 2024

ABSTRACTBackgroundIt is reported that treatment with anaplastic lymphoma kinase (ALK) tyrosine kinase inhibitors (TKIs) induces hypogonadism both in male patients with ALK‐positive cancer and in murine models.MethodsIn this study, three groups, including an experimental group of male patients with ALK‐positive, advanced non–small cell lung cancer (ANSCLC) who were receiving alectinib (cohort A), a control group of female patients with ALK‐positive ANSCLC who were receiving alectinib (cohort B), and a control group of male patients with ALK‐negative ANSCLC (cohort C), prospectively underwent a full hormone assessment for androgen deficiency at 8 weeks after the start of treatment and in case of reported suspected symptoms. Patients with major sexual dysfunctions were referred to an endocrinologist.ResultsNinety‐five patients were consecutively enrolled onto the study. Among sixty‐eight male patients, both median total testosterone levels (2.93 vs. 4.92 ng/ml; p = .0001) and free testosterone levels (0.11 vs. 0.17 pg/ml; p = .0002) were significantly lower in ALK‐positive ANSCLC patients in cohort A compared with ALK‐negative patients in cohort C; conversely, median FSH (10.32 vs. 17.52 mUI/ml; p = .0059) and LH levels (4.72 vs. 7.49 mUI/ml; p = .0131) were significantly higher in cohort C compared to cohort A. Median inhibin B levels were higher in ALK‐positive male patients (74.3 vs. 44.24 pg/ml; p = .0038), but all patients had inhibin B values within the normal range. The percentage of male patients who had positive scores on the Androgen Deficiency in Aging Males (ADAM) questionnaire was 62% in cohort A and 26.8% in cohort C, including eight patients who reported at least one major symptom and were referred to Andrology Unit. No significant differences in the endocrine assessment were reported between cohorts A and B.ConclusionsSymptoms of androgen deficiency should be tracked in male patients with ALK‐positive ANSCLC who are receiving alectinib, and testosterone replacement should be considered, as appropriate.
A TRP to Pathological Angiogenesis and Vascular Normalization
Authors: Katari Venkatesh , Dalal Kesha , Adapala Ravi K. , Guarino Brianna D. , Kondapalli Narendrababu , Paruchuri Sailaja , Thodeti Charles K. 
Published: March 2024

Abstract

Uncontrolled angiogenesis underlies various pathological conditions such as cancer, age‐related macular degeneration (AMD), and proliferative diabetic retinopathy (PDR). Hence, targeting pathological angiogenesis has become a promising strategy for the treatment of cancer and neovascular ocular diseases. However, current pharmacological treatments that target VEGF signaling have met with limited success either due to acquiring resistance against anti‐VEGF therapies with serious side effects including nephrotoxicity and cardiovascular‐related adverse effects in cancer patients or retinal vasculitis and intraocular inflammation after intravitreal injection in patients with AMD or PDR. Therefore, there is an urgent need to develop novel strategies which can control multiple aspects of the pathological microenvironment and regulate the process of abnormal angiogenesis. To this end, vascular normalization has been proposed as an alternative for antiangiogenesis approach; however, these strategies still focus on targeting VEGF or FGF or PDGF which has shown adverse effects. In addition to these growth factors, calcium has been recently implicated as an important modulator of tumor angiogenesis. This article provides an overview on the role of major calcium channels in endothelium, TRP channels, with a special focus on TRPV4 and its downstream signaling pathways in the regulation of pathological angiogenesis and vascular normalization. We also highlight recent findings on the modulation of TRPV4 activity and endothelial phenotypic transformation by tumor microenvironment through Rho/YAP/VEGFR2 mechanotranscriptional pathways. Finally, we provide perspective on endothelial TRPV4 as a novel VEGF alternative therapeutic target for vascular normalization and improved therapy. © 2024 American Physiological Society.
Compr Physiol
14:5389‐5406, 2024.
Valuation of forest‐management and wildfire disturbance on water and carbon fluxes in mountain headwaters
Authors: Chung Min Gon , Guo Han , Nyelele Charity , Egoh Benis N. , Goulden Michael L. , Keske Catherine M. , Bales Roger C. 
Published: March 2024

AbstractRecent drought, wildfires and rising temperatures in the western US highlight the urgency of increasing resiliency in overstocked forests. However, limited valuation information hinders the broader participation of beneficiaries in forest management. We assessed how historical disturbances in California's Central Sierra Nevada affected live biomass, forest water use and carbon uptake and estimated marginal values of these changes. On average, low‐severity wildfire caused greater declines in forest evapotranspiration (ET), gross primary productivity (GPP) and live biomass than did commercial thinning. Low‐severity wildfires represent proxies for prescribed burns and both function as biomass removal to alleviate overstocked conditions. Increases in potential runoff over 15 years post‐disturbance were valued at $108,000/km2 for commercial thinning versus $234,000/km2 for low‐severity wildfire, based on historical water prices. Respective declines in GPP were valued at −$305,000 and −$1,317,000/km2, based on an average social cost of carbon. Considering biomass levels created by commercial thinning and low‐severity fire as more‐sustainable management baselines for overstocked forests, carbon uptake over 15 years post‐disturbance can be viewed as a benefit rather than loss. Realizing this benefit upon management re‐entry may require sequestering thinned material. High‐severity wildfire and clearcutting resulted in greater declines in ET and thus greater potential water benefits but also substantial declines in GPP and live carbon. These lessons from historical disturbances indicate what benefit ranges from fuels treatments can be expected from more‐sustainable management of mixed‐conifer forests and the importance of setting an appropriate baseline.
Twelve‐year trends in unprotected left main coronary artery occlusion: insights from a real‐world multicentre study
Authors: Alexandre André , Campinas Andreia , Brochado Bruno , Braga Marta , Sá‐Couto David , Santos Mariana , Ribeiro Diana , Brandão Mariana , Silva Marisa Passos , de Morais Gustavo Pires , Calvão João , Silva João Carlos , Baggen‐Santos Raquel , Luz André , Silveira João , Torres Severo 
Published: March 2024

AbstractAimsAcute myocardial infarction (AMI) resulting from unprotected left main coronary artery (LMCA) occlusion and subtotal occlusion is a life‐threatening condition. Although AMI management has improved in the past two decades, there is limited information on recent trends in patient characteristics, management, and outcomes for acute unprotected LMCA‐related AMI. This study aims to assess such trends over a 12 year period.Methods and resultsThis retrospective multicentre study includes patients with unprotected LMCA occlusion/subtotal occlusion admitted to three tertiary hospitals between 2008 and 2020. The patients were divided into two groups based on the chronology of presentation: a ‘past group’ (January 2008 to December 2014) and a ‘contemporary group’ (January 2015 to December 2020). The study compares clinical characteristics, management approaches, and outcomes between the two groups. The study includes 128 patients, with 51 (40%) in the ‘past group’ and 77 (60%) in the ‘contemporary group’. Baseline risk factors did not show statistically significant differences between the two groups, except for hypertension (49% vs. 74%; P = 0.005). Chest pain was more frequent in the ‘past group’ (98% vs. 89%; P = 0.014), and a trend towards more cardiac arrests was observed in the ‘contemporary group’ (18% vs. 31%; P = 0.087). Revascularization type did not differ significantly (P = 0.419), but manual thrombectomy was less frequently used (41% vs. 23%; P = 0.032) and stent implantation showed a trend towards higher rates (66% vs. 78%; P = 0.150) in the ‘contemporary cohort’. There was a gradual shift from bare‐metal to drug‐eluting stents, with a significantly higher percentage of ticagrelor/prasugrel loading in the ‘contemporary cohort’ (5% vs. 79%; P < 0.001). The use of mechanical circulatory support (MCS), although not statistically significant, was higher among patients in the ‘past group’ (67% vs. 51%; P = 0.073). The type of MCS differed significantly between groups, with a decrease in intra‐aortic balloon pump use (67% vs. 42%; P = 0.005) and an increase in veno‐arterial extracorporeal membrane oxygenation (4% vs. 22%; P = 0.005) and Impella system (0% vs. 3%) over time. Survival analysis showed no significant differences (P = 0.599; log‐rank test) in all‐cause mortality between the different time groups, with the long‐term survival rate being approximately 30%.ConclusionsIn our real‐world population, despite the progressive use of newer drugs and more advanced devices over time, patients with unprotected LMCA occlusion/subtotal occlusion remain a subpopulation with poor prognosis.
Intravenous iron and SGLT2 inhibitors in iron‐deficient patients with heart failure and reduced ejection fraction
Authors: Docherty Kieran F. , McMurray John J.V. , Kalra Paul R. , Cleland John G.F. , Lang Ninian N. , Petrie Mark C. , Robertson Michele , Ford Ian 
Published: March 2024

AbstractAimsTo explore the potential interaction between use of SGLT2 inhibitors and the increase in haemoglobin in patients randomized to intravenous iron or the control group in the IRONMAN (Effectiveness of Intravenous Iron Treatment versus Standard Care in Patients with Heart Failure and Iron Deficiency) trial.Methods and resultsThis was a post hoc exploratory analysis of the IRONMAN trial which randomized patients with heart failure, a left ventricular ejection fraction (LVEF) ≤ 45% and iron deficiency (transferrin saturation <20% or ferritin <100 μg/L) to open label intravenous ferric derisomaltose or usual care. Of the 1137 randomized patients, 29 (2.6%) were taking an SGLT2 inhibitor at baseline. The mean (SD) change in haemoglobin from baseline at 4 weeks in those taking an SGLT2 inhibitor at baseline was 1.3 (1.2) g/dL in patients randomized to ferric derisomaltose and 0.1 (0.7) g/dL in the usual care group; between‐group difference = 1.0 g/dL (95% CI 0.1, 1.8). The equivalent numbers in the no SGLT2 inhibitor group were 0.6 (0.9) g/dL in those randomized to ferric derisomaltose and 0.1 (0.8) g/dL in the usual care group; between‐group difference = 0.4 g/dL (95% CI 0.3, 1.6); interaction P value = 0.10. No patient receiving an SGLT2 inhibitor at baseline developed polycythaemia during follow‐up (defined as haemoglobin >16.5 g/dL [men] or >16 g/dL [women]).ConclusionsIn the IRONMAN trial, there was a trend to a greater increase in haemoglobin with ferric derisomaltose in iron‐deficient patients taking an SGLT2 inhibitor at baseline, as compared with those not taking one.
Advances in Enzymatic Incorporation of Small Fluorine Modules
Authors: Lin Yuqi , Xue Wanqing , Li Hechen , Su Bingmei , Lin Juan , Ye Ke‐Yin 
Published: March 2024

AbstractOrganofluorine compounds find extensive application in the fields of agrochemicals, pharmaceuticals, materials science, and molecular imaging. Introducing fluorine atoms can provide organic compounds with unique physicochemical properties or improve their biological activity. Although significant progress has been made in the chemical synthesis of fluorine‐containing compounds, achieving selective fluorination under mild conditions remains extremely challenging. Introducing biocatalytic approaches in organofluorine chemistry is an important strategy given their high efficiency, selectivity, and environmental friendliness. In this review, we present the discovery of fluorinated natural products and fluorinases, the crystal structure and the directed evolution of fluorinases, with a focus on recent advances in the enzymatic synthesis of fluorine‐containing compounds in recent years. It is hoped that this review will help to promote the field of biocatalytic organofluorine compound synthesis.
Mainstream acculturation in superdiverse settings: The beneficial role of intergroup contact with ‘other‐cultures’ individuals
Authors: Doucerain Marina M. , Medvetskaya Anna , Benkirane Sarah , Bragoli‐Barzan Léa , Gouin Jean‐Philippe 
Published: March 2024

AbstractThis work focuses on migrants’ ‘other‐cultures’ intergroup contact – contact with individuals belonging to neither their heritage ingroup nor the mainstream outgroup. We test the overarching hypothesis that other‐cultures contact is positively associated with mainstream acculturation, especially when contact in the mainstream group is scarce. Study 1 results show that frequent other‐cultures contact is positively associated with mainstream identification among Maghrebi migrants to Quebec. This effect is stronger for participants with infrequent, versus frequent, mainstream contact. In Study 2, conducted among Russian migrants to Canada, having more other‐cultures friends is positively related to mainstream acculturation only for participants with few mainstream friends. Further, the entry of other‐cultures friends into migrants’ network takes place later than heritage friends but earlier than mainstream friends. The current work expands the traditional mainstream–heritage acculturation framework by moving away from binary conceptualizations of intergroup relations and considering other‐cultures contact in superdiverse contexts.
Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms
Authors: Jung Minseo , Lee Jinwon , Park Si Jae , Na Jeong‐Geol 
Published: March 2024

AbstractShake flask cultivation, a cornerstone in bioprocess research encounters limitations in supplying sufficient oxygen and exchanging gases, restricting its accuracy in assessing microbial growth and metabolic activity. In this communication, we introduce an innovative gas supply apparatus that harnesses the rotational motion of a shaking incubator to facilitate continuous air delivery, effectively overcoming these limitations. We measured the mass transfer coefficient (kLa) and conducted batch cultures of Corynebacterium glutamicum H36LsGAD using various working volumes to assess its performance. Results demonstrated that the gas supply apparatus significantly outperforms conventional silicone stoppers regarding oxygen delivery, with kLa values of 2531.7 h−1 compared to 20.25 h−1 at 230 rpm. Moreover, in batch cultures, the gas supply apparatus enabled substantial improvements in microbial growth, maintaining exponential growth even at larger working volumes. Compared to the existing system, an increase in final cell mass by a factor of 3.4‐fold was observed when utilizing 20% of the flask's volume, and a remarkable 9‐fold increase was achieved when using 60%. Furthermore, the gas supply apparatus ensured consistent oxygen supply and efficient gas exchange within the flask, overcoming challenges associated with low working volumes. This approach offers a simple yet effective solution to enhance gas transfer in shake flask cultivation, bridging the gap between laboratory‐scale experiments and industrial fermenters. Its broad applicability holds promise for advancing research in bioprocess optimization and scale‐up endeavors.