bluuebunny/crossref_metadata_2025_split huggingface dataset:
Data‐Independent Acquisition and Label‐Free Quantification for Quantitative Proteomics Analysis of Human Cerebrospinal Fluid
Authors: Makepeace Karl A.T. , Rookyard Alexander W. , Das Lipi , Vardarajan Badri N. , Chakrabarty Jayanta K. , Jain Anu , Kang Min Suk , Werth Emily G. , Reyes‐Dumeyer Dolly , Zerlin‐Esteves Marielba , Honig Lawrence S. , Mayeux Richard , Brown Lewis M. 
Published: March 2024

AbstractThis article presents a practical guide to mass spectrometry–based data‐independent acquisition and label‐free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Cerebrospinal fluid sample collection and preparation for mass spectrometry analysisBasic Protocol 2: Mass spectrometry sample analysis with data‐independent acquisitionSupport Protocol: Data‐dependent mass spectrometry and spectral library constructionBasic Protocol 3: Analysis of mass spectrometry data
Monitoring the Morphological Changes of Skeleton‐PtCo Electrocatalyst during PEMFC Start‐Up/Shut‐Down probed by in situ WAXS and SAXS
Authors: Janssen Marek , Drnec Jakub , Martens Isaac , Quinson Jonathan , Pittkowski Rebecca , Park Daesung , Weber Philipp , Arenz Matthias , Oezaslan Mehtap 
Published: March 2024

AbstractAdvanced in situ analyses are indispensable for comprehending the catalyst aging mechanisms of Pt‐based PEM fuel cell cathode materials, particularly during accelerated stress tests (ASTs). In this study, a combination of in situ small‐angle and wide‐angle X‐ray scattering (SAXS & WAXS) techniques were employed to establish correlations between structural parameters (crystal phase, quantity, and size) of a highly active skeleton‐PtCo (sk‐PtCo) catalyst and their degradation cycles within the potential range of the start‐up/shut‐down (SUSD) conditions. Despite the complex case of the sk‐PtCo catalyst comprising two distinct fcc alloy phases, our complementary techniques enabled in situ monitoring of structural changes in each crystal phase in detail. Remarkably, the in situ WAXS measurements uncover two primary catalyst aging processes, namely the cobalt depletion (regime I) followed by the crystallite growth via Ostwald ripening and/or particle coalescence (regime II). Additionally, in situ SAXS data reveal a continuous size growth over the AST. The Pt‐enriched shell thickening based on the Co depletion within the first 100 SUSD cycles and particle growth induced by additional potential cycles were also collaborated by ex situ STEM‐EELS. Overall, our work shows a comprehensive aging model for the sk‐PtCo catalyst probed by complementary in situ WAXS and SAXS techniques.
A thermal stress loading technique for large‐sized hot dry rock mechanical tests
Authors: Ci Huiling , Bai Bing , Lei Hongwu , Zou Yan , Liu Jianfeng 
Published: March 2024

AbstractTesting of large‐sized specimens is becoming increasingly important in deep underground rock mechanics and engineering. In traditional mechanical loading, stresses on large‐sized specimens are achieved by large host frames and hydraulic pumps, which could lead to great investment. Low‐cost testing machines clearly always have great appeal. In this study, a new approach is proposed using thermal expansion stress to load rock specimens, which may be particularly suitable for tests of deep hot dry rock with high temperatures. This is a different technical route from traditional mechanical loading through hydraulic pressure. For the rock mechanics test system of hot dry rock that already has an investment in heating systems, this technology may reduce the cost of the loading subsystem by fully utilizing the temperature changes. This paper presents the basic principle and a typical design of this technical solution. Preliminary feasibility analysis is then conducted based on numerical simulations. Although some technical details still need to be resolved, the feasibility of this loading approach has been preliminarily confirmed.
Farm management and landscape context shape plant diversity at wetland edges in the Prairie Pothole Region of Canada
Authors: Kirk David Anthony , Martínez‐Lanfranco Juan Andrés , Forsyth Douglas J. , Martin Amanda E. 
Published: March 2024

AbstractEvaluating the impacts of farming systems on biodiversity is increasingly important given the need to stem biodiversity loss, decrease fossil fuel dependency, and maintain ecosystem services benefiting farmers. We recorded woody and herbaceous plant species diversity, composition, and abundance in 43 wetland‐adjacent prairie remnants beside crop fields managed using conventional, minimum tillage, organic, or perennial cover (wildlife‐friendly) land management in the Prairie Pothole Region. We used a hierarchical framework to estimate diversity at regional and local scales (gamma, alpha), and how these are related through species turnover (beta diversity). We tested the expectation that gamma richness/evenness and beta diversity of all plants would be higher in remnants adjacent to perennial cover and organic fields than in conventional and minimum tillage fields. We expected the same findings for plants providing ecosystem services (bee‐pollinated species) and disservices (introduced species). We predicted similar relative effects of land management on alpha diversity, but with the expectation that the benefits of organic farming would decrease with increasing grassland in surrounding landscapes. Gamma richness and evenness of all plants were highest for perennial cover, followed by minimum tillage, organic, and conventional sites. Bee‐pollinated species followed a similar pattern for richness, but for evenness organic farming came second, after perennial cover sites, followed by minimum tillage and conventional. For introduced species, organic sites had the highest gamma richness and evenness. Grassland amount moderated the effect of land management type on all plants and bee‐pollinated plant richness, but not as expected. The richness of organic sites increased with the amount of grassland in the surrounding landscape. Conversely, for conventional sites, richness increased as the amount of grassland in the landscape declined. Our results are consistent with the expectation that adopting wildlife‐friendly land management practices can benefit biodiversity at regional and local scales, in particular the use of perennial cover to benefit plant diversity at regional scales. At more local extents, organic farming increased plant richness, but only when sufficient grassland was available in the surrounding landscape; organic farms also had the highest beta diversity for all plants and bee‐pollinated plants. Maintaining native cover in agroecosystems, in addition to low‐intensity farming practices, could sustain plant biodiversity and facilitate important ecosystem services.
Halogen Promoted Desulfurative Cleavage of Cyclopropylmethyl Thioethers and Amination of the Formed Cyclopropylcarbinyl Cations
Authors: Marín‐Díaz Pablo , Martínez‐Núñez Clara , Sanz Roberto , Suárez‐Pantiga Samuel 
Published: March 2024

AbstractCyclopropylmethyl sulfides react with N‐fluorosulfonimide (NFSI) or molecular iodine, enabling C−S cleavage to generate cyclopropylcarbinyl cations, which evolve through cyclopropane ring‐opening reactions into homoallyl cations suitable to react with nucleophiles present in the reaction media. This desulfurative cleavage of cyclopropylmethyl thioethers under non‐acidic conditions facilitates homoallylation of N‐based nucleophiles such as alkyl or aryl amines as well as sulfonimides through a one‐pot protocol in one or two steps depending on the nucleophile. The reaction is initiated by a halogen‐sulfur bond that causes C−S bond cleavage. Moreover, the reaction with iodine proceeds through homoallyl iodide intermediates.
A lattice‐based searchable encryption scheme with multi‐user authorization for the certificateless cloud computing environment
Authors: Zhou YiHua , Tang Bin , Yang YuGuang 
Published: March 2024

AbstractPublic key encryption with keyword search (PEKS) is able to enhance cloud data security. On the other hand, the existence of malicious cloud servers and untrusted central authorities, in addition the keyword space faces a low entropy, attackers often launch keyword guessing attacks (KGA), thereby leaking data privacy. Therefore, we use certificateless authentication and proxy re‐encryption technology to construct a lattice‐based verifiable PEKS scheme for Multi‐Data Users (MDU). Certificateless authentication can ensure that our scheme does not require key escrow, and proxy re‐encryption technology allows us to perform multi‐user authorization and use the verification block for data users to ensure the integrity of search results. Security research proves that, under the assumption that the learning with errors (LWE) problem is hard, the ciphertext is indistinguishable and resistant to KGA. Results from experiments show that our scheme is significantly more effective than current schemes.
Clinical landscape of citrin deficiency: A global perspective on a multifaceted condition
Authors: Kido Jun , Makris Georgios , Santra Saikat , Häberle Johannes 
Published: March 2024

AbstractCitrin deficiency is an autosomal recessive disorder caused by a defect of citrin resulting from mutations in SLC25A13. The clinical manifestation is very variable and comprises three types: neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD: OMIM 605814), post‐NICCD including failure to thrive and dyslipidemia caused by citrin deficiency, and adult‐onset type II citrullinemia (CTLN2: OMIM 603471). Frequently, NICCD can run with a mild clinical course and manifestations may resolve in the post‐NICCD. However, a subset of patients may develop CTLN2 when they become more than 18 years old, and this condition is potentially life‐threatening. Since a combination of diet with low‐carbohydrate and high‐fat content supplemented with medium‐chain triglycerides is expected to ameliorate most manifestations and to prevent the progression to CTLN2, early detection and intervention are important and may improve long‐term outcome in patients. Moreover, infusion of high sugar solution and/or glycerol may be life‐threatening in patients with citrin deficiency, particularly CTLN2. The disease is highly prevalent in East Asian countries but is more and more recognized as a global entity. Since newborn screening for citrin deficiency has only been introduced in a few countries, the diagnosis still mainly relies on clinical suspicion followed by genetic testing or selective metabolic screening. This paper aims at describing (1) the different stages of the disease focusing on clinical aspects; (2) the current published clinical situation in East Asia, Europe, and North America; (3) current efforts in increasing awareness by establishing management guidelines and patient registries, hereby illustrating the ongoing development of a global network for this rare disease.
Preparation of oil‐in‐water emulsions stabilized by faba bean protein–grape leaf polyphenol conjugates: pH‐, salt‐, heat‐, and freeze–thaw‐stability
Authors: Dursun Capar Tugba , Iscimen Elif Meltem , McClements David Julian , Yalcin Hasan , Hayta Mehmet 
Published: March 2024

AbstractBACKGROUNDPlant proteins are being increasingly utilized as functional ingredients in foods because of their potential health, sustainability, and environmental benefits. However, their functionality is often worse than the synthetic or animal‐derived ingredients they are meant to replace. The functional performance of plant proteins can be improved by conjugating them with polyphenols. In this study, the formation and stability of oil‐in‐water emulsions prepared using faba bean protein–grape leaf polyphenol (FP‐GLP) conjugates as emulsifiers. Initially, FP‐GLP conjugates were formed using an ultrasound‐assisted alkali treatment. Then, corn oil‐in‐water emulsions were prepared using high‐intensity sonication (60% amplitude, 10 min) and the impacts of conjugate concentration, pH, ionic strength, freezing–thawing, and heating on their physicochemical properties and stability were determined.RESULTSMicroscopy and light scattering analysis showed that oil‐in‐water emulsions containing small oil droplets could be formed at conjugate concentrations of 2% and higher. The addition of salt reduced the electrostatic repulsion between the droplets, which increased their susceptibility to aggregation. Indeed, appreciable droplet aggregation was observed at ≥ 50 mmol/L sodium chloride. The freeze–thaw stability of emulsions prepared with protein–polyphenol conjugates was better than those prepared using the proteins alone. In addition, the emulsions stabilized by the conjugates had a higher viscosity than those prepared by proteins alone.CONCLUSIONThis study showed that FP‐GLP conjugates are effective plant‐based emulsifiers for forming and stabilizing oil‐in‐water emulsions. Indeed, emulsions formed using these conjugates showed improved resistance to pH changes, heating, freezing, and salt addition. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Effect of microwave‐assisted treatment on proximate, techno‐functional, thermal, structural, and storage properties of TGN (Cyperus esculentus L.) flour
Authors: Oladunjoye Adebola O. , Olaoluwa Goodness O. 
Published: March 2024

AbstractBACKGROUNDThe use of the emerging technique of microwave‐assisted roasting on TGN (TGN) flour was investigated. Tiger nuts were subjected to microwave irradiation at 450, 600, and 900 W each at 5, 10, and 15 min, and milled to flour. The flours were analyzed for proximate, bioactive, techno‐functional, morphological, thermal, and storage effects on their composition. An untreated sample was the control.RESULTSThe results revealed that microwave treatment significantly (P < 0.05) elicited various modifications in the proximate composition and techno‐functional properties. The treatment improved the bioactive composition of phenolic content together with the antioxidant activity of the flour. Progressive microwave treatment of TGNs resulted in flours with darker colors and reduced pasting parameters. Structural modification of starch granules, protein denaturation, and starch‐protein complexes occasioned by microwave treatment were evidenced in the functional group analysis, including morphological agglomeration, increased particle size, and thermal properties. Treatment also enhanced the microbiological qualities of flour after 8 weeks of storage.CONCLUSIONThis study shows that microwave treatment produces excellent physical modifications that lead to improvements in the nutritional, functional, sensory, and color properties, and safety attributes of TGN flour for food application. This is a development that could present opportunities for novel food formulation by the food industry and related industries. © 2024 Society of Chemical Industry.
Super Broadband Emission Across NIR‐I and NIR‐II Under Blue Light Excitation of Cr3+, Ni2+ Co‐Doped Sr2GaTaO6 Phosphor Achieved by Two‐Site Occupation and Effective Energy Transfer
Authors: Zhuo Yifu , Wu Fugen , Niu Yaping , Wang Yun , zhang Qi , Teng Yun , Dong Huafeng , Mu Zhongfei 
Published: March 2024

AbstractThe performance of the near‐infrared phosphor‐converted light‐emitting diodes (NIR pc‐LEDs) mainly depends on the NIR emitting phosphors used. Cr3+ doped materials can be excited by blue light chips, but their emission is located in the NIR‐I region (650–1000 nm). Ni2+ doped materials are mainly located in the NIR‐II region (1000–1700 nm), but they cannot be effectively excited by blue light chips. Herein, Cr3+, Ni2+ mono‐doped, and co‐doped Sr2GaTaO6 NIR emitting phosphors are prepared and investigated. Cr3+ and Ni2+ ions occupy two octahedral sites of Ga3+ and Ta5+. The co‐doping of Cr3+ ions has achieved two breakthroughs. One is to shift the optimal excitation wavelength from violet light to blue light due to the energy transfer (efficiency up to 70%) from Cr3+ to Ni2+. The other is to achieve the broadband and continuous emission across NIR‐I and NIR‐II regions (650–1700 nm, with a full width at half maximum (FWHM) of 410 nm (173 nm + 237 nm)). The prepared Sr2GaTaO6: 0.02Cr3+, 0.01Ni2+ phosphor is combined with a commercial 460 nm blue chip to realize its application in organic compounds identification, night vision, and biological imaging. This work points out a direction for the future development of efficient super broadband NIR‐emitting phosphors.
It is a match! How donors and nonprofit organizations come together on a matching donation platform
Authors: Sander Philip , Zabel Julia 
Published: March 2024

AbstractOnline donation platforms have grown increasingly established in the past two decades. Among substantial research into donation‐based crowdfunding platforms in general, no studies address the intention to use matching donation platforms. The latter refers to a hypothetical product, not currently available on the market. It represents an extension of conventional donation platforms, aligning donors' preferences with NPOs and projects present on the platform. Drawing on the technology acceptance model (TAM), this exploratory study empirically investigates factors that influence the intention to use a matching donation platform. It also extends the TAM to include trust and donation processing factors and considers the perspectives of both donors and nonprofit organizations (NPOs) engaged in fundraising. The data include an online survey of 458 donors and 112 NPOs in Germany. The findings indicate that all factors, including trust and donation processing, within the extended TAM exert a significant impact on the intention to use a matching donation platform, and attitude toward the platform represents the most influential factor. This article also provides entrepreneurial recommendations for how a matching donation platform can be implemented and used in practice. For example, a matching donation platform should aim at younger donor target groups and provide NPOs with suitable options for processing donations.
Prenatal diagnosis of ROR‐2 related Robinow syndrome presenting with fetal ultrasound findings of mesomelia, vertebral, digital and genital abnormalities
Authors: Yang Liying , Shannon Patrick , Silver Rachel , Roifman Maian , Yates Carin , Chitayat David 
Published: March 2024

AbstractAutosomal recessive ROR2‐Robinow syndrome is caused by pathogenic variants in the ROR2 gene. Fetal ultrasound done on our patient at 24 + 3/7 weeks gestation showed macrocephaly, brachycephaly, flat face, prominent forehead, mild frontal bossing, lower thoracic hemivertebrae, digital abnormalities and micropenis. Fetal trio whole exome sequencing done on amniocytes showed two pathogenic compound heterozygous variants in the ROR2 gene, c.1324 C > T; p.(Arg442*) maternally inherited and c.1366dup; p.(Leu456Profs*3) apparently de novo. c.1324 C > T; p.(Arg442*) is a nonsense variant resulting in protein truncation reported to be associated with RRS3. c.1366dup; p.(Leu456Profs*3) is a frameshift variant predicted to result in protein truncation reported to segregate with the disease in multiple affected individuals from a single large family with distal symphalangism of the fourth finger. Fetal autopsy following pregnancy termination showed a large head with low‐set ears, facial abnormalities, mesomelic bone shortening, hemivertebra, fused S3 and S4 vertebral bodies, several fused rib heads and short penis with buried shaft.
Effects of Storage Temperatures and Times on Drying Chicken Breasts Using Film‐Drying: A New Application of Polymeric Film for Food Preservation
Authors: Alnadari Fawze , Al‐Dalali Sam , Sallam Abubakr , Nasiru Mustapha Muhammad , Korma Sameh A. , Abdin Mohamed , Baiome Baiome Abdelmaguid , Abdalmegeed Dyaaaldin , Chen Chao , Zeng Xiaoxiong 
Published: March 2024

ABSTRACTThis present study reports the effect of different storage temperatures on the film‐dried chicken breast (FDCB) by film‐drying technique for 15 days, including FDCB‐(−18), FDCB‐4, FDCB‐18, FDCB‐28 and FDCB‐38 (°C). Both storage time and temperature negatively affected the water‐holding capacity and moisture ratio, except for FDCB‐(−18). To ensure the quality of film‐dried chicken meats, they were rehydrated and evaluated in terms of colour values, tissue microstructure and hardness. The results showed that the rehydrated samples FDCB‐28 and FDCB‐38 promoted redness loss and increased the hardness values compared with other samples at low temperatures. Moreover, the storage temperature below 28°C was the most effective strategy for best maintaining the physicochemical, antioxidative, microbiological and sensory quality of film‐dried chicken samples, similar to those of raw meat. Interestingly, the FDCB‐(−18), FDCB‐4 and FDCB‐18 samples showed a significant (p < 0.05) inhibitory effect on microbial growth and lipid oxidation for 15 days.
Failure prediction of mechanical system based on meta‐action
Authors: Ran Yan , Chen Jingjie , Sagor Nafis Jawyad , Zhang Genbao 
Published: March 2024

AbstractHighly reliable mechanical systems can lead to significant losses in the event of failure, and the lack of comprehensive failure data presents challenges for developing techniques such as critical part identification and failure prediction. In light of this, this paper proposes a meta‐action‐based fault prediction method that effectively addresses the issue of limited fault data. Initially, the mechanical system is decomposed utilizing the “Function‐Motion‐Action” (FMA) methodology to derive individual meta‐action units (MAUs). Subsequently, the limited sample of fault data from the mechanical system is combined with processed expert knowledge to construct the corresponding fault propagation‐directed graph. Furthermore, the key MAUs are determined by applying the Decision–Making Trial and Evaluation Laboratory (DEMATEL) method. Last, the degradation data of the key MAUs is acquired by monitoring them, and a non‐homogeneous discrete grey model (DNGM) integrated with an improved BP neural network is proposed to facilitate the fault prediction of MAUs. Using an industrial robot as a case study, the prediction results demonstrate the superiority of the method proposed in this paper over a single gray model and neural network, thereby providing a reliable prediction approach for anticipating the future trends of data‐deficient mechanical systems.