bluuebunny/crossref_metadata_2025_split huggingface dataset:
QSPR Study of n‐Octanol/Water Partition Coefficient of Some Aromatic Compounds Using Support Vector Regression
Authors: Yang Shan‐Sheng , Lu Wen‐Cong , Gu Tian‐Hong , Yan Liu‐Ming , Li Guo‐Zheng 
Published: December 2008

AbstractQuantitative Structure–Property Relationship (QSPR) model was developed to correlate structures of aromatic compounds with their n‐octanol–water partition coefficient (logKow). The 68 molecular descriptors derived solely from the structures of the aromatic compounds were calculated using Gaussian 03, HyperChem 7.5, and TSAR V3.3. The descriptors were screened by the minimum Redundancy Maximum Relevance (mRMR)‐Genetic Algorithm (GA)‐Support Vector Regression (SVR) method. The parameters of the SVR model were optimized using the five‐fold cross‐validation method. The QSPR model was developed from a training set consisting of 300 compounds using the SVR method with a good determination coefficient (R2=0.85). The QSPR model was then tested using an external test set consisting of 50 compounds with satisfactory external predictive ability (q2=0.84). The results show that mRMR‐GA‐SVR feature selection method and SVR method can be used to model logKow for a diverse set of aromatic compounds and could be promising tools in the field of QSPR research.
XPS study of the effect of aluminium on the atmospheric corrosion of the AZ31 magnesium alloy
Authors: Feliu Jr S. , Merino M. C. , Arrabal R. , Coy A. E. , Matykina E. 
Published: December 2008

AbstractThe surface characteristics and corrosion behaviour of the AZ31 magnesium alloy exposed to a high relative humidity (RH) atmosphere were investigated. During the first 15 days of humidity test at 98% RH and 50 °C, a significant increase of magnesium carbonate and a decrease of magnesium oxide were detected on the surface film by XPS; after this stage, increased exposure times did not produce substantial changes on the relative amounts of these compounds. The surface film of commercially pure magnesium, also examined for comparison purposes, revealed more magnesium carbonate and less magnesium oxide compared with the AZ31 alloy. Unlike the AZ31 alloy, the surface of pure Mg disclosed almost complete substitution of MgO by magnesium carbonate after 30 days of exposure time. Mass gain values of tested specimens and scanning electron microscope characterisation of corroded surfaces indicated lower corrosion susceptibility of the AZ31 alloy compared with the commercially pure Mg, suggesting superior chemical stability of the oxide/hydroxide film formed over the magnesium–aluminium alloy surface. XPS and energy dispersive X‐ray (EDX) analyses did not revealed any substantial enrichment of aluminium in the corrosion products film on the AZ31 alloy after 30 days of testing. Copyright © 2008 John Wiley & Sons, Ltd.
Estimating diagnostic accuracy of multiple binary tests with an imperfect reference standard
Authors: Albert Paul S. 
Published: December 2008

AbstractThe goal in diagnostic medicine is often to estimate the diagnostic accuracy of multiple experimental tests relative to a gold standard reference. When a gold standard reference is not available, investigators commonly use an imperfect reference standard. This paper proposes methodology for estimating the diagnostic accuracy of multiple binary tests with an imperfect reference standard when information about the diagnostic accuracy of the imperfect test is available from external data sources. We propose alternative joint models for characterizing the dependence between the experimental tests and discuss the use of these models for estimating individual‐test sensitivity and specificity as well as prevalence and multivariate post‐test probabilities (predictive values). We show using analytical and simulation techniques that, as long as the sensitivity and specificity of the imperfect test are high, inferences on diagnostic accuracy are robust to misspecification of the joint model. The methodology is demonstrated with a study examining the diagnostic accuracy of various HIV‐antibody tests for HIV. Published in 2008 by John Wiley & Sons, Ltd.
A new method for DNA extraction from feces and hair shafts of the South China tiger (Panthera tigris amoyensis)
Authors: Zhang Wenping , Zhang Zhihe , Xu Xiao , Wei Kun , Wang Xiaofang , Liang Xu , Zhang Liang , Shen Fujun , Hou Rong , Yue Bisong 
Published: December 2008

AbstractIt is commonly known that tigers (Panthera tigris) groom themselves by licking their coats, which leads to an abundance of hairs in their feces. These hairs are designated specially as “fecal hairs”. In our study, in order to explore fecal hairs potential as a DNA source for genetic analysis, 55 fecal hair samples were collected from 23 captive South China tigers (P. t. amoyensis). According to the amplification of mitochondrial primers loop F and loop R, DNA quality of noninvasive samples were grouped into three grades: grade I—the highest‐quality DNA, grade II—high‐quality DNA, and grade III—poor‐quality DNA. No failed amplifications on microsatellite primers and only 0.27% genotyping errors occurred with grade I fecal hair DNA, as compared with 9.4% failed amplifications on microsatellite primers and 9.5% genotyping errors with grade II fecal hair DNA. It was found that 25.45% of fecal hair DNA was grade I and 65.45 and 10.00% of fecal hair DNA were grades II and III, respectively, as compared with 4.35% grade I fecal DNA and 34.78 and 60.87% grades II and III fecal DNA, respectively. Thus, higher‐quality DNA can be extracted from fecal hairs than feces. In addition, DNA could be extracted from hair shafts of tigers and a minimum of 2000 hair shafts were required for visible DNA bands on a 1% agarose gel. These findings demonstrate that fecal hairs may serve as a convenient and reliable genomic DNA source for genotype analysis. Zoo Biol 28:49–58, 2009. © 2008 Wiley‐Liss, Inc.
Multiple shRNA‐mediated knockdown of TACE reduces the malignancy of HeLa cells
Authors: Yan Yuan , Zhang Jie , Guo Jian‐Li , Huang Wei , Yang Yu‐Zhen 
Published: December 2008

AbstractTumor necrosis factor‐alpha (TNF‐α) converting enzyme (TACE) is a key enzyme involved in the proteolytic shedding of the ectodomain of several membrane‐bound growth factors, cytokines and receptors. Here, we constructed a multiple short hairpin RNA (shRNA) expression vector containing four shRNAs against TACE. We found that in HeLa cells our multiple shRNAs vector produced a higher level of TACE knockdown than any single shRNA vector containing only one TACE shRNA. Silencing TACE expression in HeLa cells decreased their malignancy by decreasing the proliferation, adhesion and migration, as well as inducing apoptosis in these cells. Furthermore, our data suggest that the effects of TACE on the malignancy of HeLa cells may be mediated via activation of the EGFR (epidermal growth factor receptor) signaling pathway. Our findings suggest that using a combination of shRNAs within one vector to silence the expression of TACE might be a potential therapeutic strategy for tumors.
Epiblast isolation by a new four stage method (peeling) from whole bovine cloned blastocysts
Authors: Kwon Dae Kee , Hong So Gun , Park Hee Jung , Kang Jung Taek , Koo Ok Jae , Lee Byeong Chun 
Published: December 2008

AbstractWe have established a new 4 stage epiblast isolation method from whole bovine cloned blastocysts without using immunosurgery. The new “peeling” method consists of dissolution of the zona pellucida (first stage), elimination of mural trophoblast (second stage), isolation of primitive endoderm and epiblast from polar trophoblast (third stage), and isolation of epiblast from primitive endoderm (fourth stage). The bovine cloned blastocyst consists of 4 different types of cells showing abundant alkaline phosphatase activity. The epiblast origin of isolated cells was confirmed by in vitro differentiation of isolated cells to tubulin β3‐positive neurons and by embryoid body formation. The bovine cloned blastocyst origin of isolated epiblasts was confirmed by microsatellite analysis and mitochondrial DNA sequencing analysis. This new method might accelerate establishment of somatic cell nuclear transfer derived embryonic stem cell lines from bovine and other mammals.
Factors impacting on doctors’ management of acute low back pain: A systematic review
Authors: Fullen Brona M. , Baxter G. David , O'Donovan Barry G.G. , Doody Catherine , Daly Leslie E. , Hurley Deirdre A. 
Published: December 2008

ABSTRACTThe aim of this review was to determine the factors that impact on doctors’ management of patients with acute low back pain. A methodological assessment of databases (Medline, EMBASE, Psychinfo, BIOSIS, CINAHL, and the Cochrane Central Register of Controlled Trials) identified papers which were screened for inclusion criteria by two independent reviewers. Data were extracted from accepted papers, and the internal validity and strength of the evidence were determined using valid and reliable scales.The search generated a total of 28 papers [quantitative (n=27), qualitative (n=1) methodologies]. Themes were identified from the accepted papers: education (n=18), knowledge of clinical guidelines and impact on management (n=7), and doctors’ demographics (n=4). There was consistent evidence that doctors did not adhere to clinical guidelines when performing a spinal assessment. There was inconsistent evidence that education increased adherence with acute LBP guideline recommendations in terms of referral rates to physiotherapy, for investigations, to secondary care and for maintaining patients at work. Strategies to address the factors impacting on doctors’ management of acute LBP are required; these would lead to improvement in patient outcomes and reduce healthcare costs.
Ultrabroadband electromagnetic wave propagation in hydrocarbon reservoirs in the presence of an oil-water interface
Authors: Epov M.I. , Mironov V.L. , Komarov S.A. , Muzalevskii K.V. 
Published: December 2008

Abstract
Numerical experiments are applied to simulate propagation of broadband pulses which are transmitted by an electric dipole and travel in a uniform oil-bearing formation or in a layered reservoir with an oil-water interface. We calculate the frequency responses of the oil- and water-saturated layers as low-frequency filters for the propagating waves and find the respective cutoff frequencies. Attenuation rates are analyzed in the cases of incident nanosecond and picosecond pulses in uniform oil-bearing formations and those reflected from the oil-water interface. The low frequency filtering of pulses is investigated in terms of its effect on wave energy attenuation during propagation in oil-bearing rocks.
A new method of controlled-source frequency induction soundings
Authors: Zhuravleva R.B. , Mironov D.G. 
Published: December 2008

Abstract
We suggest a new inversion method for frequency induction data implying the use of a new parameter, which has a simple analytical form in the case of a uniform subsurface. The new parameter is found from induction numbers measured in the field of a vertical magnetic dipole or a vertical magnetic dipole combined either with a horizontal electrical dipole or with a horizontal magnetic dipole. Compared with the classical methods, the new technique provides better resolved resistivity curves and faithful images of the subsurface at higher frequencies and smaller transmitter-receiver separations. The inversion algorithm is applied to amplitude and amplitude-phase data and provides reliable depth assignment of the detected resistivity layers in the latter case.
Permo-Triassic volcanics of the Koltogory-Urengoi rift of the West Siberian geosyneclise
Authors: Saraev S.V. , Baturina T.P. , Ponomarchuk V.A. , Travin A.V. 
Published: December 2008

Abstract
The lithologic composition of basalts from the most complex sections of the Koltogory-Urengoi Rift is considered, and their 40Ar/39Ar ages are given: basalts from the lower section of Nikol’skaya BH-1 (Bergamak Formation) — 272.9 ± 10.5 and 249.5 ± 3.0 Ma, basalts from the uppermost section (Voinov Formation) — 247.5 ± 2.9 Ma, and basalts from the lower section of Tyumenskaya SDB-6 (Korotchaevo Formation) — 251.2 ± 2.0 Ma. According to these and the paleomagnetic data, trap magmatism began in the Koltogory-Urengoi Rift in the Permian and ended in the Early Triassic, whereas the paleontological data suggest its beginning in the Early Triassic and its termination in the late Middle Triassic.
In Tyumenskaya SDB-6 we have recognized both low-K tholeiitic and medium- to high-K subalkalic and calc-alkalic basalts, which form large alternating packets up to 480 m thick. This section is juxtaposed to the anomalous sections of the Siberian Platform trap formation near the margins of the Tunguska syneclise. The section of Nikol’skaya BH-1 is composed mainly of medium- and high-K subalkaline basalts with scarce flows of low-K ones. The low-K basalts differ from the medium- and high-K varieties in having lower contents of TiO2, P2O5, Ba, Rb, Th, and ΣLREE.
The Tyumenskaya SDB-6 basalts are characterized by paleohypergene alterations, up to the development of more than five levels of alumina-ferruginous cuirasses, as well as superposed chloritization, sericitization (hydromication), carbonatization, zeolitization, silicification, and epidotization, whereas the Nikol’skaya BH-1 basalts are subjected mainly to carbonatization, chloritization, and hydromication. We have concluded that the existence of a vast mega-arch above the superplume within the West Siberian geosyneclise in the Permo-Triassic was complicated by the Koltogory-Urengoi Rift. The Triassic rift deposits are both continental and marine sediments (sandstones and siltstones with glauconite, coccolith oozes, spherulitic lavas, and hyaloclastites). The basic volcanism within the rift showed the features of both rift and flood basalts as well as E-MORB.
Paleoarchean tonalite-trondhjemite complex in the northwestern part of the Sharyzhalgai uplift (southwestern Siberian craton): results of U-Pb and Sm-Nd study
Authors: Turkina O.M. , Berezhnaya N.G. , Larionov A.N. , Lepekhina E.N. , Presnyakov S.L. , Saltykova T.E. 
Published: December 2008

Abstract
In the northwestern part of the Sharyzhalgai uplift of the Siberian craton (Bulun block), the earliest sialic crust (grey-gneiss complex) is composed of plagiogneisses, their migmatized varieties, and subordinate plagiogranitoids. The petrochemical, trace-element, and Sm-Nd isotope compositions of rocks were studied, and U-Pb dating of zircons (SHRIMP II) was performed. Plagiogneisses and plagiogranitoids of trondhjemite and, more seldom, tonalite compositions are predominant; their compositions are typical of rocks of Archean tonalite-trondhjemite-granodiorite (TTG) complexes (Al2O3 ≥ 15%, Mg# = 28–38, (La/Yb)n = 23–66, Sr/Y = 27–135, Eu/Eu* = 0.7–1.1). Plagiogneisses of meta-andesite-rhyodacite association are subordinate (SiO2 = 59–69%, (La/Yb)n = 7–32, Sr/Y = 11–24, Eu/Eu* = 0.5–0.7). Cathodoluminescent study of zircons revealed “magmatic cores” and metamorphic rims; most of the rims differ from the cores in U and Th contents and low or greatly varying Th/U ratios. In migmatized plagiogneisses of trondhjemite composition, two zircon generations of different morphologies have been recognized. The protoliths of the grey-gneiss complex rocks formed in the Paleoarchean as a result of two discrete magmatic events, at ∼3.3 and 3.25 Ga, and their metamorphism and migmatization took place at ∼3.2 Ga. The isotopic and geochemical features of rocks evidence that the primary melts were produced mainly through the melting of metabasic sources at different depths of the thickened crust. Plagiogneisses of trondhjemite composition apparently resulted from magma generation involving ancient sialic material.
Rare-earth elements as indicators of ore sources and the degree of differentiation and ore potential of rare-metal granite intrusions (eastern Transbaikalia)
Authors: Kozlov V.D. 
Published: December 2008

Abstract
The meteoritic-material-normalized REE patterns of rare-metal granite intrusions of the ore-bearing Kukul’bei complex (J2–J3), eastern Transbaikalia, were studied. It is shown that the intrusions were initially enriched in granitophile volatiles and trace elements (rare metals), i.e., this phenomenon is not related to the differentiation of their parental magma chambers. On the differentiation of the Kukul’bei rare-metal intrusions, the REE contents decrease in passing from granites of the main intrusive phase (MP) to late leucocratic differentiates (muscovite and amazonite granites), the differentiates become more enriched in granitophile elements, and their rare-metal contents drastically increase as compared with the MP granites. The ore-bearing bodies of muscovite and amazonite granites have extremely low REE contents and the highest contents of granitophile (including ore-forming) elements.
The REE patterns of the Kukul’bei intrusive differentiates are not universal among rare-metal intrusions. By the example of highly ore-bearing rare-metal granite intrusions of the Erzgebirge, Central Europe, it has been established that their late deep-seated differentiates (ultrarare-metal lithionite-zinnwaldite Li-F-granites) accompanied by highly productive Sn-W mineralization concentrate both granitophile elements and REE (particularly HREE). Among the studied Transbaikalian rare-metal intrusions of the Kukul’bei complex, only the differentiates of the most ore-bearing Sherlovaya Gora intrusive system belong to the above type. The analysis of the REE patterns of the Kukul’bei granites confirmed the earlier conclusions on the low ore potential of the rare-metal mineralization of the studied intrusive complex.
A macroseismic study of the Taldy-Sai caravanserai in the Kara-Bura River valley (Talas basin, Kyrgyzstan)
Authors: Korjenkov A.M. , Tabaldiev K.Sh. , Bobrovskii Al.V. , Bobrovskii Ar.V. , Mamyrov E.M. , Orlova L.A. 
Published: December 2008

Abstract
We report a macroseismic study of a ruined medieval building, a small caravanserai located along the caravan way from the Talas valley to the Chatkal and Fergana valleys in Kyrgyzstan. The ruins document several events in which the caravanserai was destroyed, apparently during earthquakes, and was rebuilt or repaired. The earliest earthquake occurred soon after the building was put up, about 400 years ago, according to radiocarbon dating of charcoal, and another event shook the area between 400 and 50 years BP. After being damaged in the ultimate earthquake, in the middle of the 20th century, the building was eventually abandoned.
In situ measurements of capelin (Mallotus villosus) target strength in the North Pacific Ocean
Authors: Guttormsen Michael A. , Wilson Christopher D. 
Published: December 2008

Abstract
Guttormsen, M. A. and Wilson, C. D. 2009. In situ measurements of capelin (Mallotus villosus) target strength in the North Pacific Ocean. – ICES Journal of Marine Science, 66: 258–263. In situ measurements of capelin (Mallotus villosus) target strength (TS) were collected during summer 2001–2003 near Kodiak Island in the Gulf of Alaska, using a calibrated EK500 echosounder with 38 and 120 kHz split-beam transducers. Targets were detected over dispersed, night-time aggregations using standard acoustic methods, then filtered using a quality-control algorithm to reject invalid targets. The 38 kHz-based, fitted model estimate was TS = 20 log10L− 70.3 (r2 = 0.30), where L is total length of fish. Compared with other studies, the TS-fitted model at 38 kHz was similar to that calculated from swimbladder morphology measurements from St Lawrence estuary capelin (TS = 20 log10L− 69.3), but resulted in greater estimates than models based on in situ measurements of capelin TS in the Barents Sea (TS = 19.1 log10L−74.0) and northern Atlantic Ocean (TS = 20 log10L − 73.1). The large intraspecific variability exhibited in the fitted TS – L models for this species suggests the use of TS measurements from the geographic region where the data were collected.
CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function
Authors: Serra Olga , Soler Marçal , Hohn Carolin , Sauveplane Vincent , Pinot Franck , Franke Rochus , Schreiber Lukas , Prat Salomé , Molinas Marisa , Figueras Mercè 
Published: December 2008

Abstract
Suberin is a cell wall lipid polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was down-regulated in potato plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 ω-hydroxyacid (approximately 70%) and α,ω-diacid (approximately 90%) monomers in comparison with wild type. Moreover, the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type. Thus, our data provide convincing evidence for the involvement of ω-functional fatty acids in establishing suberin structure and function.
A Rice Kinase-Protein Interaction Map
Authors: Ding Xiaodong , Richter Todd , Chen Mei , Fujii Hiroaki , Seo Young Su , Xie Mingtang , Zheng Xianwu , Kanrar Siddhartha , Stevenson Rebecca A. , Dardick Christopher , Li Ying , Jiang Hao , Zhang Yan , Yu Fahong , Bartley Laura E. , Chern Mawsheng , Bart Rebecca , Chen Xiuhua , Zhu Lihuang , Farmerie William G. , Gribskov Michael , Zhu Jian-Kang , Fromm Michael E. , Ronald Pamela C. , Song Wen-Yuan 
Published: December 2008

AbstractPlants uniquely contain large numbers of protein kinases, and for the vast majority of the 1,429 kinases predicted in the rice (Oryza sativa) genome, little is known of their functions. Genetic approaches often fail to produce observable phenotypes; thus, new strategies are needed to delineate kinase function. We previously developed a cost-effective high-throughput yeast two-hybrid system. Using this system, we have generated a protein interaction map of 116 representative rice kinases and 254 of their interacting proteins. Overall, the resulting interaction map supports a large number of known or predicted kinase-protein interactions from both plants and animals and reveals many new functional insights. Notably, we found a potential widespread role for E3 ubiquitin ligases in pathogen defense signaling mediated by receptor-like kinases, particularly by the kinases that may have evolved from recently expanded kinase subfamilies in rice. We anticipate that the data provided here will serve as a foundation for targeted functional studies in rice and other plants. The application of yeast two-hybrid and TAPtag analyses for large-scale plant protein interaction studies is also discussed.