bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advances in the Study of Necroptosis in Vascular Dementia: Focus on Blood–Brain Barrier and Neuroinflammation
Authors: Qiu Yuemin , Cheng Lin , Xiong Yinyi , Liu Ziying , Shen Chunxiao , Wang Liangliang , Lu Yujia , Wei Shufei , Zhang Lushun , Yang Seung Bum , Zhang Xiaorong 
Published: February 2025

ABSTRACTBackgroundVascular dementia (VaD) includes a group of brain disorders that are characterized by cerebrovascular pathology.Neuroinflammation, disruption of the blood–brain barrier (BBB) permeability, white matter lesions, and neuronal loss are all significant pathological manifestations of VaD and play a key role in disease progression. Necroptosis, also known asprogrammed necrosis, is a mode of programmed cell death distinct from apoptosis and is closely associated with ischemic injury and neurodegenerative diseases. Recent studies have shown that necroptosis in VaD exacerbates BBB destruction, activates neuroinflammation, promotes neuronal loss, and severely affects VaD prognosis.Results and ConclusionsIn this review, we outline the significant roles of necroptosis and its molecular mechanisms in the pathological process of VaD, with a particular focus on the role of necroptosis in modulating neuroinflammation and exacerbating the disruption of BBB permeability in VaD, and elaborate on the molecular regulatory mechanisms and the centrally involved cells of necroptosis mediated by tumor necrosis factor‐α in neuroinflammation in VaD. We also analyze the possibility and specific strategy that targeting necroptosis would help inhibit neuroinflammation and BBB destruction in VaD. With a focus on necroptosis, this study delved into its impact on the pathological changes and prognosis of VaD to provide new treatment ideas.
Sirtuin1 in Spinal Cord Injury: Regulatory Mechanisms, Microenvironment Remodeling and Therapeutic Potential
Authors: Li Jinze , Cui Shengyu , Li Yanqiu , Zhang Can , Chang Chao , Jian Fengzeng 
Published: February 2025

ABSTRACTBackgroundSpinal cord injury (SCI) is a complex central nervous system disorder characterized by multifaceted pathological processes, including inflammation, oxidative stress, programmed cell death, autophagy, and mitochondrial dysfunction. Sirtuin 1 (Sirt1), a critical NAD+‐dependent deacetylase, has emerged as a promising therapeutic target for SCI repair due to its potential to protect neurons, regulate glial and vascular cells, and optimize the injury microenvironment. However, the regulatory roles of Sirt1 in SCI are complex and challenging, as its effects vary depending on activation timing, expression levels, and cell types.MethodsA systematic literature review was conducted using PubMed, Scopus, and Web of Science to identify studies investigating Sirt1 in SCI. Relevant publications were analyzed to synthesize current evidence on Sirt1's mechanisms, therapeutic effects, and challenges in SCI repair.ResultsSirt1 exerts broad regulatory effects across diverse pathological processes and cell types post‐SCI. It promotes neuronal survival and axonal regeneration, modulates astrocytes and microglia to resolve inflammation, supports oligodendrocyte‐mediated myelination, and enhances vascular endothelial function. Proper Sirt1 activation may mitigate secondary injury, whereas excessive or prolonged activation could impair inflammatory resolution or disrupt cellular homeostasis. This review highlights Sirt1 activation as potential therapies, but challenges include optimizing spatiotemporal activation and addressing dual roles in different cell types.ConclusionTargeting Sirt1 represents a viable strategy for SCI repair, given its multifaceted regulation of neuroprotection, immunomodulation, and tissue remodeling. However, translating these findings into therapies requires resolving critical issues such as cell type‐specific delivery, precise activation timing, and dosage control. This review provides a theoretical foundation and practical insights for advancing Sirt1‐based treatments for SCI.
Ciliary IFT‐B Transportation Plays an Important Role in Human Endometrial Receptivity Establishment and is Disrupted in Recurrent Implantation Failure Patients
Authors: Yang Haoxuan , Zhang Jing , Yan Fei , Chen Yihong , Wu Yang , Luo Jiaxin , Duan Lian , Zou Juan , Guo Juncen , Pang Jiyun , Dinnyes Andras , Zeng Jiuzhi , Liu Weixin , Wang Chi Chiu , Lin Yi , Xiao Xue , Zhao Xiaomiao , Xu Wenming 
Published: February 2025

ABSTRACTThe lack of accurate understanding of cellular physiology and pathophysiology during the WOI constitutes the major obstacle to correct diagnosis and treatment for patients with recurrent implantation failure (RIF). The role of cilia as one of the key organelles in endometrial epithelium has been poorly understood during embryo implantation. In this study, the morphological and molecular changes of endometrial cilia regulated by hormones were demonstrated in endometrial epithelial organoid models. Multi‐omics studies revealed highly relevant cilia‐related activities like cilia movement during endometrial receptivity establishment. Interestingly, both in vitro model and in vivo patient data have shown that the apical part of cilium formed a cilia‐derived spherical structure after hormone stimulation. We also found intraflagellar transport (IFT) train multi‐subunit complex B (IFT‐B) was aggregated in the sphere during the implantation window. Meanwhile mitochondria localization signal increased at the cilia basement. Proteomics and the functional assay showed the deficiency of energy metabolism in RIF patients and cilia formation abnormalities. The abnormal energy supply resulted in the failure of vesicle transport by deficiency of IFT‐B location, ultimately leading to the failure of receptivity establishment. Our study revealed the essential role of endometrial cilia in embryo implantation and indicated that mitochondrial metabolism was crucial for normal ciliogenesis and embryo implantation.
Erwerbstätigkeit, Arbeitsfähigkeit und Krankenstand bei Patienten mit Melanom im ersten Jahr nach der Diagnose
Authors: Dugas‐Breit Susanne , Dugas Martin , Schulze Hans‐Joachim 
Published: February 2025

ZusammenfassungHintergrundDas maligne Melanom betrifft jüngere Berufstätige. In dieser Studie wurden Arbeitsfähigkeit und Krankenstand innerhalb eines Jahres nach Diagnosestellung untersucht, sowie der Einfluss von Rehabilitations‐ und psychoonkologischen Maßnahmen betrachtet.Patienten und MethodikAn dieser monozentrischen, prospektiven Beobachtungsstudie nahmen 221 Patienten (62,4% weiblich) im Alter von 19 bis 65 Jahren teil. 78,5% hatten zu Beginn Tumorstadium IB oder niedriger. Die routinemäßige klinische Dokumentation, Berufsanamnese, Arbeitsfähigkeit, subjektive Prognose der Beschäftigung sowie der Bedarf an und Zufriedenheit mit Rehabilitationsmaßnahmen wurden wiederholt erhoben.Ergebnisse181 Patienten (82%) waren bei Erstvorstellung erwerbstätig, 172 (78%) beim letzten Besuch. Etwa 75% der Patienten schätzten ihre Arbeitsfähigkeit zunächst als „mäßig“ ein, und hatten bis zu 9 Krankheitstage. Im darauffolgenden Jahr nahmen die Krankheitstage bei Patienten im Stadium IB und IIA signifikant zu (p = 0,044), bei Patienten im Stadium IIB und höher hoch signifikant (p < 0,001). Psychoonkologische Beratung (24%) und Rehabilitation (18%) änderten die signifikant schlechter eingeschätzte Arbeitsfähigkeit dieser Patienten nicht.SchlussfolgerungenDas Melanom beeinträchtigt die Arbeitsfähigkeit, auch in Tumorstadien ohne Lymphknotenbefall oder Fernmetastasen. Kontrollierte klinische Studien wären sinnvoll, um den Erfolg von Rehabilitationsmaßnahmen und psychoonkologischen Interventionen bei Patienten mit Melanom zu evaluieren.
Evidenz‐ und konsensbasierte (S3) Leitlinie: Management der Urethritis bei männlichen* Jugendlichen und Erwachsenen
Authors: Werner Ricardo Niklas , Vader Isabell , Abunijela Susan , Bickel Markus , Biel Anika , Boesecke Christoph , Branke Lisa , Bremer Viviane , Brockmeyer Norbert Hermann , Buder Susanne , Esser Stefan , Heuer Ruben , Köhn Frank‐Michael , Mais Andrea , Nast Alexander , Pennitz Antonia , Potthoff Anja , Rasokat Heinrich , Sabranski Michael , Schellberg Sven , Schmidt Axel Jeremias , Schmidt Sebastian , Schneidewind Laila , Schubert Sören , Schulte Caroline , Spinner Christoph , Spornraft‐Ragaller Petra , Sunderkötter Cord , Vester Udo , Zeyen Christoph , Jansen Klaus 
Published: February 2025

ZusammenfassungDie Urethritis ist ein häufiges, überwiegend durch sexuell übertragene Erreger wie Chlamydia trachomatis, Neisseria gonorrhoeae oder Mycoplasma genitalium verursachtes Krankheitsbild. Alleine aufgrund klinischer Merkmale ist die Unterscheidung der Erreger und der Ausschluss von Koinfektionen nicht sicher möglich. In der praktischen Versorgungssituation wird jedoch oftmals, noch vor dem Erregernachweis, eine empirische antibiotische Therapie durchgeführt. Ziel dieser evidenzbasierten S3‐Leitline ist die Förderung einer rationalen Syndrom‐orientierten Herangehensweise an das klinische Management männlicher Jugendlicher und Erwachsener mit Symptomen einer Urethritis.Neben Empfehlungen für Diagnostik, Klassifikation und Therapie beinhaltet die Leitlinie Empfehlungen zur Indikationsstellung für die empirische antibiotische Behandlung der penilen Urethritis. Neu gegenüber vorbestehenden, erregerspezifischen Leitlinien ist unter anderem das Flowchart für das Syndrom‐orientierte praktische Management. Bei Indikation zur empirischen Behandlung und Verdacht auf eine gonorrhoische Urethritis soll Ceftriaxon angewandt werden. Zusätzlich sollte, aufgrund des Risikos für eine Koinfektion mit Chlamydia trachomatis, Doxycyclin verordnet werden, es sei denn, eine Wiedervorstellung zur Behandlung möglicher Koinfektionen ist gesichert. Bei Verdachtsdiagnose einer nichtgonorrhoischen Urethritis soll die empirische Therapie mit Doxycyclin erfolgen. Azithromycin ist zur empirischen Behandlung der gonorrhoischen und nichtgonorrhoischen penilen Urethritis nur einzusetzen, wenn Kontraindikationen gegenüber Doxycyclin bestehen. Detaillierte Empfehlungen zur Differenzialdiagnostik, erregerspezifischen Behandlungen, spezifischen Situationen und zur Beratung und Nachsorge finden sich in der Leitlinie.
Climate Shame: What Is It, Does It Matter, and How Do We Handle It?
Authors: Christoffersen Mikkel Gabriel 
Published: February 2025

ABSTRACTThe green transition touches most aspects of contemporary human lives, including their emotions. This article explores one particular climate emotion, namely shame, asking three interrelated questions: What is climate shame? How does climate shame contribute to the green transition? And within which Christian theological context might we handle climate shame when we experience it? For each question, the article employs different methods, thereby combining phenomenology, ethics, and systematic theology. By exploring climate shame, the article contributes to an interdisciplinary theological understanding of our emotional life in the green transition.
The relationship of plasma glucose, stress hyperglycaemia and glycated haemoglobin with intermediate‐term mortality after acute myocardial infarction in patients with and without diabetes
Authors: Montebello Annalisa , Agius Martina , Grech Martina , Maniscalco Nicoletta , Kenkovski Ivana , Fava Stephen 
Published: February 2025

AbstractAimsAdmission hyperglycaemia is an adverse prognostic indicator in the setting of acute myocardial infarction. It is unclear if this is because of previously undiagnosed diabetes, due to admission plasma glucose being a marker of a greater stress hormone response and therefore a more severe event, or due to a detrimental effect of high blood glucose on the myocardium.MethodsWe performed retrospective analysis of a cohort of 430 participants admitted with a diagnosis of acute myocardial infarction. We investigated the relation of admission plasma glucose, glycated haemoglobin, stress hyperglycaemia ratio and a novel parameter; the stress hyperglycaemia index, to mortality by Cox regression analyses. The stress hyperglycaemia index is the difference between the admission glucose and glucose estimated from HbA1c.ResultsWe included 430 participants who were followed for a median of 2.5 years. The stress hyperglycaemia index was associated with increased mortality in patients with diabetes in both univariate and multivariate analyses. Admission glucose was associated with mortality in subjects with diabetes in univariate analysis and after adjustment of age and sex, but not after adjusting for eGFR. There was no significant association between admission glucose and mortality in subjects without diabetes. HbA1c was not associated with mortality in either patient group in both univariate and multivariate analyses.ConclusionOur results suggest a possible detrimental effect of hyperglycaemia in the setting of an acute myocardial infarction.
A Trade‐Off Between Antimicrobial Peptide Resistance and Sensitivity to Host Immune Effectors in Staphylococcus aureus In Vivo
Authors: El Shazely Baydaa , Rolff Jens 
Published: February 2025

ABSTRACTAntimicrobial peptides (AMPs) are essential immune effectors of multicellular organisms. Bacteria can evolve resistance to AMPs. Surprisingly, when used to challenge the yellow mealworm beetle, Tenebrio molitor, Staphylococcus aureus resistant to an abundant AMP (tenecin 1) of the very same host species did not increase host mortality or bacterial load compared to infections with wild‐type S. aureus. A possible explanation is that antimicrobial resistance is costly due to the collaterally increased sensitivity of AMP‐resistant strains to other immune effectors. Here, we study the sensitivity of a group of AMP‐resistant S. aureus strains (resistant to tenecin 1 or a combination of tenecin 1 + 2) to other immune effectors such as phenoloxidase and other AMPs in vivo. Using RNAi‐based knockdown, we investigate S. aureus survival in insect hosts lacking selected immune effectors. We find that all except one AMP‐resistant strain displayed collateral sensitivity toward phenoloxidase. Some AMP‐resistant strains show sensitivity to components of the yellow mealworm beetle AMP defense cocktail. Our findings are consistent with the idea that resistance to AMPs does not translate into changes in virulence because it is balanced by the collaterally increased sensitivity to other host immune effectors. AMP resistance fails to provide a net survival advantage to S. aureus in a host environment that is dominated by AMPs.
No‐Reference Image Quality Assessment: Past, Present, and Future
Authors: Mao Qingyu , Liu Shuai , Li Qilei , Jeon Gwanggil , Kim Hyunbum , Camacho David 
Published: February 2025

ABSTRACTNo‐reference image quality assessment (NR‐IQA) has garnered significant attention due to its critical role in various image processing applications. This survey provides a comprehensive and systematic review of NR‐IQA methods, datasets, and challenges, offering new perspectives and insights for the field. Specifically, we propose a novel taxonomy for NR‐IQA methods based on distortion scenarios and design principles, which distinguishes this work from previous surveys. Representative methods within each category are thoroughly examined, with a focus on their strengths, limitations, and performance characteristics. Additionally, we review 20 widely used NR‐IQA datasets that serve as benchmarks for evaluating these methods, providing detailed information on the number of images, distortion types, and distortion levels for each dataset. Furthermore, we identify and discuss key challenges currently faced by NR‐IQA methods, such as handling diverse and complex distortions, ensuring generalisation across datasets and devices, and achieving real‐time performance. We also suggest potential future research directions to address these issues. In summary, this survey offers a comprehensive and systematic examination of NR‐IQA methods, datasets, and challenges, offering valuable insights and guidance for researchers and practitioners working in the NR‐IQA domain.
Combining Eddy Covariance Towers, Field Measurements, and the MEMS 2 Ecosystem Model Improves Confidence in the Climate Impacts of Bioenergy With Carbon Capture and Storage
Authors: Falvo Grant , Zhang Yao , Abraha Michael , Mosier Samantha , Su Yahn‐Jauh , Lei Cheyenne , Chen Jiquan , Cotrufo M. Francesca , Robertson G. Philip 
Published: February 2025

ABSTRACTCarbon dioxide removal technologies such as bioenergy with carbon capture and storage (BECCS) are required if the effects of climate change are to be reversed over the next century. However, BECCS demands extensive land use change that may create positive or negative radiative forcing impacts upstream of the BECCS facility through changes to in situ greenhouse gas fluxes and land surface albedo. When quantifying these upstream climate impacts, even at a single site, different methods can give different estimates. Here we show how three common methods for estimating the net ecosystem carbon balance of bioenergy crops established on former grassland or former cropland can differ in their central estimates and uncertainty. We place these net ecosystem carbon balance forcings in the context of associated radiative forcings from changes to soil N2O and CH4 fluxes, land surface albedo, embedded fossil fuel use, and geologically stored carbon. Results from long term eddy covariance measurements, a soil and plant carbon inventory, and the MEMS 2 process‐based ecosystem model all agree that establishing perennials such as switchgrass or mixed prairie on former cropland resulted in net negative radiative forcing (i.e., global cooling) of −26.5 to −39.6 fW m−2 over 100 years. Establishing these perennials on former grassland sites had similar climate mitigation impacts of −19.3 to −42.5 fW m−2. However, the largest climate mitigation came from establishing corn for BECCS on former cropland or grassland, with radiative forcings from −38.4 to −50.5 fW m−2, due to its higher plant productivity and therefore more geologically stored carbon. Our results highlight the strengths and limitations of each method for quantifying the field scale climate impacts of BECCS and show that utilizing multiple methods can increase confidence in the final radiative forcing estimates.
GGR Handbook of Rock and Mineral Analysis Chapter 2 (Part 1) Analysis of Geological Materials – 1: Sample Preparation Methods
Authors: Robinson Philip 
Published: February 2025

This chapter (Analysis of Geological Materials – 1: Sample Preparation Methods) is a contribution to the Geostandards and Geoanalytical Research Handbook of Rock and Mineral Analysis – an online textbook that is a fully revised and updated edition of A Handbook of Silicate Rock Analysis (P. J. Potts, 1987, Blackie, Glasgow).In Chapter 2, Part 1 (from Section 1 of the handbook dealing with fundamentals of measurement and instrument design) rock sample preparation techniques will be considered in detail (Parts 2 and 3 deal with pre‐concentration and separation procedures, and the determination of specific element groups, respectively). Despite some techniques undertaking measurements on solid samples, the need for dissolution remains the preferred method for many modern instrumental measurement principles such as ICP‐MS, ICP‐AES and TIMS. A solution provides homogeneity and can further be used for separation and pre‐concentration, both of which are important for the measurement of low mass fractions in isotope geochemistry. Part 1 first takes an historical view of developments in this field, followed by state‐of‐the‐art assessments of dissolution procedures based on acid attack, decomposition by molten salt fusion, the decomposition of resistant minerals, flux‐free fusion, and the use of pressed powder pellets.
Reference Intervals and Comparative Analysis of Reticulocyte Counts Using the Mindray BC‐6200, Flow Cytometry, and Manual Microscopy
Authors: Aphaiwiwat Hathaiphon , Ketloy Chutitorn , Thepnarin Tapakorn , Watanaboonyongcharoen Phandee , Prompetchara Eakachai 
Published: February 2025

ABSTRACTIntroductionThis study evaluated the analytical performance of the Mindray BC‐6200 analyzer for reticulocyte counting, with a focus on imprecision, carryover, and time stability. The accuracy of reticulocyte count was compared with the manual microscope (MM) and flow cytometry (FC), the reference method. Additionally, reference intervals (RIs) of reticulocyte count and related parameters were established for the Thai population.MethodsSixty healthy Thai adults of both sexes along with 182 leftover blood samples from individuals with various pathologic conditions, were selected to evaluate reticulocyte counts using the automated Mindray BC‐6200. Results were compared with MM and FC to establish RIs and assess the correlation between methods.ResultsThe imprecision on reticulocyte counts across all control levels (coefficient of variation, %CV) was below the manufacturer's claim. Carryover was < 0.001%, and time‐stability was excellent up to 24 h. RIs were as follows: reticulocytes (×109/L): 34.88–118.50, immature reticulocyte fraction (IRF) (%): 2.71–15.28, reticulocyte Hemoglobin content (RHE) (pg): 22.84–29.70. There was a strong correlation (r > 0.9785) in reticulocyte counts between the automated analyzer, MM, and FC.ConclusionThe Mindray BC‐6200 is a reliable alternative to MM and FC methods for reticulocyte counting, with a good correlation, precision, low carryover, and time stability, making it effective for assessing erythropoiesis in clinical settings. However, further studies are needed to evaluate the clinical utility of reticulocyte count and related parameters in diagnosing hematological conditions.
Developmental Relationships Between Early Vocabulary Acquisition, Joint Attention and Parental Supportive Behaviors
Authors: Wengman Johan , Forssman Linda 
Published: February 2025

ABSTRACTIn late infancy and early toddlerhood, joint attention ability is widely recognized as a crucial foundation for children's vocabulary development, though the exact nature of its contribution remains debated. This study investigates associations between joint attention and subsequent vocabulary development, as well as the possible moderating role of supportive parental behaviors. Seventy children and their families participated in this longitudinal study, which began when the children were 10 months of age. Parents completed the Swedish Communicative Development Inventory (CDI) at four age points (10, 12, 18, and 24 months) to assess receptive and expressive vocabulary growth. Children participated in lab‐based assessment of joint attention abilities at 10, 12, and 18 months. Additionally, at 10 and 12 months, parent‐child dyads participated in two semi‐structured lab assessments to evaluate the quality of parental supportive behaviors during interactions with their child. Primary analysis showed no significant effects of joint attention on subsequent receptive and expressive vocabulary. However, a significant interaction was found between a child's ability to respond to joint attention cues and parental supportive behaviors on receptive vocabulary. These findings indicate that parental supportive behaviors play a crucial role in promoting the development of children's receptive vocabulary.