bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conventional and microfluidic methods: Design and optimization of lipid-polymeric hybrid nanoparticles for gene therapy
Authors: González-García Daniel , Tapia Olga , Évora Carmen , García-García Patricia , Delgado Araceli 
Published: February 2024

Abstract

Gene therapy holds significant promise as a therapeutic approach for addressing a diverse range of diseases through the suppression of overexpressed proteins and the restoration of impaired cell functions. Developing a nanocarrier that can efficiently load and release genetic material into cells remains a challenge. In this study, lipid-polymeric hybrid nanoparticles (LPHNPs) with PLGA, DC-cholesterol, and DOPE-mPEG2000 were produced by single-step nanoprecipitation (SSN) and microfluidic (MF) methods. The optimized nanoparticles by SSN have a size of 149.9 ± 18.07 nm, a polydispersity index (PdI) of 0.23 ± 0.02, and a zeta potential of (ZP) of 29.34 ± 2.44 mV, while by MF the size was 179.8 ± 6.3, a PdI of 0.24 ± 0.01, and a ZP of 32.25 ± 1.36 mV. Furthermore, LPHNPs prepared with GapmeR-protamine by both methods exhibit a high encapsulation efficiency of approximately 90%. The encapsulated GapmeR is completely released in 24 h. The LPHNP suspensions are stable for up to 6 h in 10% FBS at pH 5.4 and 7.4. By contrast, LPHNPs remain stable in suspension in 4.5% albumin at pH 7.4 for 24 h. Additionally, LPHNPs are successfully freeze-dried using 2.5 and 5% trehalose for long-term storage. The LPHNPs produced by MF and SSN increase 1.8–3.2 fold GapmeR cell uptake, respectively. They also endosomally escape in approximately 80%. The developed LPHNPs will be useful for targeting gene therapies.
Exploring the Impact of Smog on Healthy Male Rat Physiology: A Holistic Approach Integrating Behavior, Immunology, Histopathology, and Antibiotic- Resistant Gene
Authors: Khan Muhammad Imran , Amin Arshia 
Published: February 2024

Abstract
This study has investigated the repercussions of extended exposure to smog on the behavioral, immunological, and histopathological dimensions in healthy male rats. The research utilized an experimental design with two groups, each comprising 12 rats. Group I, identified as the control group, was further divided into subgroups (A-Islamabad & B-Lahore) and placed in a smog-free environment. Group II, the test group, was also subdivided into subgroups (A-Islamabad & B-Lahore) and exposed to a smog-laden atmosphere for a 60-day duration. Following this exposure period, comprehensive behavioral and immunological testing was conducted. Subsequently, the rats were humanely euthanized through cervical dislocation for an in-depth histopathological analysis of lung tissues. Behavioral assessments included the open field test, evaluating locomotion, anxiety, and exploratory tendencies. The Y-maze test assessed spontaneous alternation and working memory. These evaluations aimed to unveil the impact of smog exposure on the mental well-being of the rats. Immunological analyses involved the collection of blood samples for the assessment of levels of CD4 and CD8, providing insights into oxidative stress in both control and test groups. Following the comprehensive testing, the animals were sacrificed, and lung tissues were meticulously separated for subsequent histopathological examination. Further, metagenomic analysis of smog-exposed rat lung tissues reveal antibiotic resistant gene profiles. The research has successfully revealed potential alterations in behavior, immunological, and histopathology induced by prolonged smog exposure in healthy male rats. The outcomes of this study contribute significantly to our understanding of the health implications associated with environmental smog pollution.
Study on the influence of Amorphophallus muelleri and flue-cured tobacco rotation on the soil microbial community in Southwest China
Authors: 农 晚意 , Lu Chao , Li Hongyu , Lu Wei , Liu Shuai , Zhang Tiehuai , Liu Fawei , Liu Zengjie , Huang Feiyan , Lin Jiang , Yu Lei 
Published: February 2024

Abstract
The continuous cropping of tobacco soil is an obstacle in current flue-cured tobacco production. To explore the effects of the different planting patterns on soil nutrients and microbial community structure, soil samples from the Amorphophallus muelleri continuous cropping (MMT1), A. muelleri + flue-cured tobacco rotation (MKT2) and flue-cured tobacco continuous cropping (KKT3) systems were collected at the same experimental site. The characteristics and relationships between the soil microbial community and soil nutrients under the different planting patterns were analyzed via Illumina MiSeq high-throughput sequencing. The results showed that the soil organic matter content, hydrolyzable nitrogen content and pH value in the A. muelleri + flue-cured tobacco rotation treatment were significantly higher than those in the other two treatments. The composition of the soil microbial communities under the different planting patterns was similar at the phylum level, but the relative abundances of Proteobacteria, Mortierellomycota and Basidiomycota in the A. muelleri + flue-cured tobacco rotation treatment were 0.31, 0.56 and 0.39 times higher than those in A. muelleri continuous cropping and flue-cured tobacco continuous cropping, respectively. The abundance of Ascomycota in the flue-cured tobacco continuous cropping treatment was 0.02 times higher than that in the A. muelleri continuous cropping of A. muelleri + flue-cured tobacco (P < 0.05). At the genus level, the abundances of Sphingomonas, Arthrobacter, Bacillus and Mortierella in the A. muelleri + flue-cured tobacco rotation treatment were 0.84, 0.006, 0.36 and 0.07 times higher than those in the A. muelleri continuous cropping, and 0.78, 1.41 and 0.24 times higher than those in the flue-cured tobacco continuous cropping treatment. The dominant bacteria and fungi were significantly correlated with the soil nutrients. From the perspective of microbial function, the bacterial KEGG enrichment pathway of the tested soil samples was mainly the metabolism pathway. The energy metabolism, carbohydrate and amino acid gene abundances of the soil bacterial community after A. muelleri + flue-cured tobacco rotation were higher than those after A. muelleri continuous cropping and flue-cured tobacco continuous cropping. The rotation planting pattern plays a positive role in regulating soil nutrients, improving the microbial community structure and increasing the functional metabolism of beneficial bacteria. The results of this study can provide a reference for the production of high-quality flue-cured tobacco.
In Silico Drug Discovery: Unveiling Potential Targets in Plasmodium falciparum through Molecular Docking Analysis
Authors: Murugesan  , kaleeswaran  
Published: February 2024

Abstract
The most significant human health problem is malaria, caused by the Plasmodium parasite and affecting nearly half of the global population. Malaria results in an annual death toll ranging from 1.2 to 2.7 million worldwide. Consequently, there is a pressing need for novel active ingredients with targeted effects to curb the worldwide spread of malaria. The objective of the present research was to explore innovative pharmacological molecules and employ bioinformatics methods for the development of effective anti-malarial drugs. As part of the latest anti-malarial chemical development, our study identified seven drug combinations from various databases demonstrating drug-like properties and robust anti-malarial activity in silico. Dioncophyllin-A, hugorosenone, marmesine, oxyprotostemonin, pachyrrhizin, plumbagin, and stemocurtisin were subjected to docking against the hexokinase-1 protein (PDB: 1CZA). Among the pachyrrhizin compounds, the one with the highest docking score (-9.9 kcal/mol) was directed towards the 1CZA protein. Through superimposing the target and template structures, the active centres of the hexokinase I protein were identified, revealing structurally identical folds and undoubtedly conserved active sites. The SWISS-ADME tool was employed to assess the excellent absorption, distribution, metabolism, and excretion (ADME) properties of the investigated drug candidates. In summary, our research identifies seven potential anti-malarial drug combinations with strong in silico activity. We've elucidated their interaction with the hexokinase-1 protein and assessed their favourable pharmacokinetic properties. These findings represent a significant step toward developing effective treatments for malaria, emphasizing the importance of further experimental validation and clinical studies.
a Care Regime Typology of Elder, Long-Term Care Institutions
Authors: Damme Maike van , Spijker Jeroen , Pavlopoulos Dimitris 
Published: February 2024

Abstract

This study generates a classification of 26 European OECD countries with respect to care regimes. We build upon the literature on the dimensions of defamilialization and familialism and empirically test how these two dimensions indicate different types of care regimes. Using Latent Profile Analysis, we group the 26 countries based on five indicators of institutional elderly care. These indicators cover formal elder care support in care in kind as well as in financial support. The results reveal three care regime types: ‘defamilialized’; ‘medium familialism’; and ‘familialism-by-default’. This classification contributes to developing a theoretical framework of care institutions.
Paclitaxel-eluting stents versus paclitaxel-coated balloons in coronary artery disease: a meta-analysis of randomized controlled trials
Authors: De Liyis Bryan Gervais , Aryaweda Made Dhiyo Wiweka , Suastika Luh Oliva Saraswati 
Published: February 2024

The efficacy of drug-coated balloons (DCB) versus drug-eluting stents (DES) for coronary artery disease (CAD) remains inconclusive. Despite paclitaxel's common use in both DES and DCB, there is a lack of meta-analyses comparing paclitaxel-eluting stents (PES) and paclitaxel-coated balloons (PCB). This meta-analysis aims to evaluate and compare DES and DCB outcomes using paclitaxel. A Systematic literature search in Medline and Cochrane databases yielded six randomized controlled trials with 951 patients (1:1 ratio). Primary endpoints were mortality, target lesion vascularization (TLV), myocardial infarction (MI), target vessel revascularization (TVR), and major adverse cardiovascular events (MACEs). Secondary endpoints included in-device binary stenosis, in-segment binary stenosis, late luminal loss (LLL), post minimal lumen diameter (MLD), and post diameter stenosis. Within the study populations, the incidence of previous MI was significantly lower in the PES group compared to the PCB group (26.70% vs. 39.22%, OR:0.56, 95%CI:0.41–0.76, p=0.0002). The meta-analysis results showed that mortality (OR:1.57, 95%CI:0.67-3.66, p=0.29), TLV (OR:0.74, 95%CI:0.37-1.48, p=0.39), MI (OR:1.76, 95%CI:0.79-3.88, p=0.16), TVR (OR:0.76, 95%CI:0.51-1.12, p=0.16), and MACEs (OR:1.11, 95%CI:0.48-2.58, p=0.81) did not exhibit significant differences between PES and PCB group in CAD. Furthermore, in stent or in balloon binary stenosis (OR:0.80, 95%CI:0.34-1.87, p=0.60), in segment binary stenosis (OR:1.16, 95%CI:0.48-2.80, p=0.74), LLL (MD:0.03, 95%CI:-0.11 to 0.17, p=0.65), post MLD (MD:0.04, 95%CI:-0.23 to 0.30, p=0.77), and post diameter stenosis (MD:-5.48, 95%CI:-13.88 to 2.92, p=0.20) were similar in both groups. Our comprehensive analysis concludes that both PES and PCB manifest comparable effectiveness and safety in the management of CAD.
Identification of surfactin lipopeptides isoforms produced by a newly isolated strain Bacillus sp. MI27 with potential use as main ingredients in detergent formulation: Application of mixture design for the formulation of a bio-based detergent
Authors: Mnif Ines , Ghoul Marwa , Bouassida Mouna , Segovia Roser , Ghribi Dhouha , Rabanal Anglada Francesc 
Published: February 2024

Abstract
The purification and characterization of lipopeptide biosurfactants produced by Bacillus sp. MI27 permits to identify two clusters of cyclic and linear surfactin isoforms. Aiming for a potential application in industrial biotechnology, we studied its potential washing capacity. The results show a good ability to wash oil stains, motor oil and mayonnaise evaluated by the determination of the % of stain removal and tissue bleaching indices after washing. Optimal washing capacities were unregistered for 300 and 400 mg/L at pH values ranging from 5 to 9 and temperatures ranging from 20 to 40°C. Using a 4-factor mixing design, we optimized a liquid formula composed only of BioS; silicate; carboxymethyl cellulose and calcium phosphate. The washing efficiency of the formula is of the order of 43.46% for petroleum and 49.10% for motor oil with a percentage reduction in surface tension of about 55.79% corresponding to 25.7 mN/m.
Development of MXene for removal of lead (Pb+) ions from wastewater
Authors: Kagalkar Abhishek , Dharaskar Swapnil , Chaudhari Nitin 
Published: February 2024

Abstract

A new family of 2D materials with enhanced properties than graphene plays a crucial role in environmental remediation and sustainability. The concentration of heavy metal ions in wastewater is an emerging issue in the global scenario. Heavy metals such as lead, chromium, cadmium, etc. are natural carcinogens and adversely affect environmental species, which tend to have potential activities on the surface of the new families. Lead poisoning is a very raising issue in some parts of India and is a major effluent disposed of to the waterbodies from various industrial streams. The removal of the lead components is studied in this research using adsorption experimentation using MXene as the surface-active adsorbent. The possible mechanism for lead removal from wastewater is also discussed, as well as the effective regeneration and reusability of the adsorbent over long adsorption cycles. MXenes were applied after the confirmation using X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and zeta-potential. The induced coupled plasma–optical emission spectroscopy confirms the lead remediation by over 96% in the first adsorption cycle, which was then reduced to over 92% in the second adsorption cycle. The lead contamination was under the permissible limits as per the water standards.
Assessment of the impact of land cover changes on sediment yield: a case study
Authors: Güvel Şerife Pınar 
Published: February 2024

Abstract

Land use is one of the most important variables impacting river basin water and land resource planning and management. Sediment transport in streams is an important issue in the assessment of sediment deposition in dam reservoirs and its impact on operational activities. Evaluation of sediment yield contributes to decision-makers for effective water management and protection of the dam body and its elements. In this study, land use cover changes, in particular in forest areas, agricultural areas, arable lands, permanent crops, heterogeneous agricultural areas, and pastures in the Çakıt and Körkün sub-basins of the Seyhan River basin were examined between 1990 and 2018 using CORINE land use cover (CLC) data. The effect of land use/land cover (LULC) modifications on sediment yield was studied using suspended sediment observation data and CLC data. It was determined that the forest area in the Çakıt sub-basin drainage area increased between 1990 and 2018, and decreased in the Körkün sub-basin. It was determined that the increase in forest area in the Çakıt sub-basin drainage region until 2018 helped to reduce sediment movement. The regression values between forest area and suspended sediment concentration in Çakıt and Körkün sub-basins were found to be 0.9521 and 0.4431, respectively.
User Experience Evaluation of a Spinal Surgery Robot: Workload, Usability, and Satisfaction Study
Authors: Choi Hyeonkyeong , Kim Seunghee , Jang Wonseuk 
Published: February 2024

Background
Robotic spine surgery has continued to evolve since its US Food and Drug Administration approval in 2004, with products now including real-time video guidance and navigation during surgery. As the market for robotic surgical devices evolves, it is important to consider usability factors.

Objective
The primary objective of this study was to determine the user experience of a surgical-assistive robotic device. The secondary objective was to evaluate workload, usability, the After-Scenario Questionnaire (ASQ), and the System Usability Scale (SUS). In addition, this study compares the workload, usability, and satisfaction survey of the device among different occupational groups using the device.

Methods
Doctors (n=15) and nurses (n=15), the intended users of the surgical assistant robot, participated in the usability evaluation. Participants performed essential scenarios for the surgical assistant robot and provided scenario-specific satisfaction (ASQ), workload (NASA Task Load Index), and usability (SUS) scores.

Results
Both doctors and nurses had task success rates of 85% or higher for each scenario. ASQ results showed that both doctors and nurses were least satisfied with ease of completing the task of registration (group 1: mean 4.73, SD 1.57 and group 2: mean 4.47, SD 1.8), amount of time it took (group 1: mean 4.47, SD 1.63 and group 2: mean 4.40, SD 2.09), and support information satisfaction (group 1: mean 5.13, SD 1.50 and group 2: mean 5.13, SD 1.89). All participants had low workloads, and the overall Task Load Index score had a P value of .77, which is greater than .05. The SUS results showed that the overall usability mean for doctors was 64.17 (SD 16.52) and the mean for nurses was 61.67 (SD 19.18), with a P value of .84, which is greater than .05, indicating no difference between the 2 groups.

Conclusions
In this study, doctors and nurses evaluated the interaction of the device in a simulated environment, the operating room. By evaluating the use experience and usability of the device with real intended users, we can develop a more effective and convenient user interface.
Editorial
Authors: Sushartami Wiwik 
Published: February 2024

Tourism has served as and remains a significant driver of national development in many countries. Adopting a critical perspective in the field of tourism studies towards this circumstance will be advantageous for both the tourism sector and academia. The Gadjah Mada Journal of Tourism Studies contributes to the broader field of tourism studies, which aims to enhance the prosperity of the worldwide community. The current edition of the Gadjah Mada Journal of Tourism Studies contains the five articlesMemorable Tourism Experience (MTE) pada Wisatawan di Perkemahan Waduk SermoPeran Komunitas Seni dan Budaya dalam Pengembangan Desa Mandiri Budaya di Desa Ekowisata PancohProses Adaptasi Komunitas Wisata untuk Mencapai Ketahanan di Kampung Wisata Rejowinangun Yogyakarta Selama Pandemi Covid-19Strategi Mengatasi Aksesibilitas Destinasi Pulau Kecil Dengan Penyesuaian Segmentasi Wisatawan Kepulauan RiauStrategi Pengelolaan Sektor Pariwisata oleh Pokdarwis Desa Wisata Srikeminut pada Masa Krisis Pandemi COVID-19
Surgical repair and replacement for native mitral valve infective endocarditis
Authors: Malvindi Pietro Giorgio , Luthra Suvitesh , Zingale Anna , Bifulco Olimpia , Berretta Paolo , Pierri Michele Danilo , Ohri Sunil K. , Di Eusanio Marco 
Published: February 2024

Aims
The clinical benefits of mitral valve repair over replacement in the setting of mitral infective endocarditis are not clearly established.

Methods
Data of patients who underwent cardiac surgery for infective endocarditis over a 20-year period (2001–2021) at two cardiac centres were reviewed. Among them, 282 patients underwent native mitral valve surgery and were included in the study. Nearest-neighbour propensity-score matching was performed to account for differences in patients’ profile between the repair and replacement subgroups.

Results
Mitral valve replacement was performed in 186 patients, while in 96 cases patients underwent mitral valve repair. Propensity match analysis provided 89 well matched pairs. Mean age was 60 ± 15 years; 75% of the patients were male. Mitral valve replacement was more commonly performed in patients with involvement of both mitral leaflets, commissure(s) and mitral annulus. Patients with lesion(s) limited to P2 segment formed the majority of the cases undergoing mitral valve repair. There was no difference in terms of microbiological findings. In-hospital mortality was 7% with no difference between the repair and the replacement cohorts. Survival probabilities at 1, 5 and 10 years were 88%, 72% and 68%, respectively after mitral repair, and 88%, 78% and 63%, respectively after mitral replacement (log-rank P = 0.94).

Conclusions
Mitral valve repair was more commonly performed in patients with isolated single leaflet involvement and provided good early and 10-year outcomes. Patients with annular disruption, lesion(s) on both leaflets and commissure(s) were successfully served on early and mid-term course by mitral valve replacement.
Cardiac imaging correlates and predictors of stroke in patients with atrial fibrillation: a meta-analysis
Authors: Noubiap Jean Jacques , Nyaga Ulrich Flore , Middeldorp Melissa E. , Stokes Michael B. , Sanders Prashanthan 
Published: February 2024

Background
New nonclinical parameters are needed to improve the current stroke risk stratification schemes for patients with atrial fibrillation. This study aimed to summarize data on potential cardiac imaging correlates and predictors of stroke or systemic embolism in patients with atrial fibrillation.

Methods
MEDLINE, EMBASE, and Web of Science were searched to identify all published studies providing relevant data through 16 November 2022. Random effects meta-analysis method was used to pool estimates.

Results
We included 64 studies reporting data from a pooled population of 56 639 patients. Left atrial spontaneous echo-contrast [adjusted odds ratio (aOR) 3.32, 95% confidence interval (CI) 1.98–5.49], nonchicken wing left atrial appendage (LAA) morphology (aOR 2.15, 95% CI 1.11–4.18), left atrial enlargement (aOR 2.12, 95% CI 1.45–3.08), and higher LAA orifice diameter (aOR 1.56, 95% CI 1.18–2.05) were highly associated with stroke. Other parameters associated with stroke included higher left atrial sphericity (aOR 1.14, 95% CI 1.01–1.29), higher left atrial volume (aOR 1.03, 95% CI 1.01–1.04), higher left atrial volume index (aOR 1.014, 95% CI 1.004–1.023), lower left atrial reservoir strain [adjusted hazard ratio (aHR) 0.86, 95% CI 0.76–0.98], higher left ventricular mass index (aOR 1.010, 95% CI 1.005–1.015) and E/e’ ratio (aOR 1.12, 95% CI 1.07–1.16). There was no association between LAA volume (aOR 1.37, 95% CI 0.85–2.21) and stroke.

Conclusion
These cardiac imaging parameters identified as potential predictors of thromboembolism may improve the accuracy of stroke risk stratification schemes in patients with atrial fibrillation. Further studies should evaluate the performance of holistic risk scores including clinical factors, biomarkers, and cardiac imaging.