bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lithium chloride promotes mesenchymal‐epithelial transition in murine cutaneous wound healing via inhibiting CXCL9 and IGF2
Authors: Zhang Chong , Luo Xiao , Wei Mianxing , Jing Bingshuai , Wang Jue , Lin Lanling , Shi Bing , Zheng Qian , Li Chenghao 
Published: April 2024

AbstractCutaneous wound healing is a challenge in plastic and reconstructive surgery. In theory, cells undergoing mesenchymal transition will achieve re‐epithelialization through mesenchymal‐epithelial transition at the end of wound healing. But in fact, some pathological stimuli will inhibit this biological process and result in scar formation. If mesenchymal‐epithelial transition can be activated at the corresponding stage, the ideal wound healing may be accomplished. Two in vivo skin defect mouse models and dermal‐derived mesenchymal cells were used to evaluate the effect of lithium chloride in wound healing. The mesenchymal‐epithelial transition was detected by immunohistochemistry staining. In vivo, differentially expressed genes were analysed by transcriptome analyses and the subsequent testing was carried out. We found that lithium chloride could promote murine cutaneous wound healing and facilitate mesenchymal‐epithelial transition in vivo and in vitro. In lithium chloride group, scar area was smaller and the collagen fibres are also orderly arranged. The genes related to mesenchyme were downregulated and epithelial mark genes were activated after intervention. Moreover, transcriptome analyses suggested that this effect might be related to the inhibition of CXCL9 and IGF2, subsequent assays demonstrated it. Lithium chloride can promote mesenchymal‐epithelial transition via downregulating CXCL9 and IGF2 in murine cutaneous wound healing, the expression of IGF2 is regulated by β‐catenin. It may be a potential promising therapeutic drug for alleviating postoperative scar and promoting re‐epithelialization in future.
Terrestrial subsidies and light affect an aquatic micro‐ecosystem in unexpected ways
Authors: Rogy Pierre , Srivastava Diane S. 
Published: April 2024

Abstract

The effects of resource subsidies on a recipient ecosystem depends on a variety of factors. Firstly, increasing the supply of limiting nutrients tends to increase the accumulation of biomass across different compartments of the recipient food web. Secondly, in the case of aquatic microorganisms, this accumulation may also be contingent on inputs of energy through light, mediating interactions between heterotrophic bacteria and autotrophic algae. Thirdly, macroscopic consumers feed upon microorganisms and recycle nutrients through excretion and egestion, profoundly affecting nutrient cycling.

In order to examine the context‐dependency of nutrient cycling in aquatic systems, we performed an experiment in mesocosms where we followed the responses of dissolved nutrients, microorganisms, and larvae of the cosmopolitan mosquito Culex quinquefasciatus (Diptera: Culicidae) to two terrestrial subsidies differing in carbon (C):nutrient, in combination with two light intensities.

We found that the interactions amongst bacteria and algae themselves remained generally unimportant after accounting for treatment effects, and that light did mediate some of the effects of nutrient subsidies on microorganisms. We also found that the presence of mosquito larvae was associated with altered concentrations of nitrogen (N) in some treatments, ultimately benefitting algae. Finally, we found that low C:nutrient subsidies generally increased the development rate of mosquito larvae, and that increased light exposure led to higher mosquito survival.

Overall, these patterns may be explained by strong nitrogen limitation in our system, causing both group of microorganisms to respond similarly to variations in nutrient supply without direct covariation, and to the key role of N recycling by mosquito larvae in sustaining algal densities in exposed treatments.

The context‐dependency in our study may reflect elemental or molecular constraints other than phosphorus. Our experiment thus calls for greater synthesis amongst ecological theories.
Bringing traits back into the equation: A roadmap to understand species redistribution
Authors: Comte Lise , Bertrand Romain , Diamond Sarah , Lancaster Lesley T. , Pinsky Malin L. , Scheffers Brett R. , Baecher J. Alex , Bandara R. M. W. J. , Chen I‐Ching , Lawlor Jake A. , Moore Nikki A. , Oliveira Brunno F. , Murienne Jerome , Rolland Jonathan , Rubenstein Madeleine A. , Sunday Jennifer , Thompson Laura M. , Villalobos Fabricio , Weiskopf Sarah R. , Lenoir Jonathan 
Published: April 2024

AbstractEcological and evolutionary theories have proposed that species traits should be important in mediating species responses to contemporary climate change; yet, empirical evidence has so far provided mixed evidence for the role of behavioral, life history, or ecological characteristics in facilitating or hindering species range shifts. As such, the utility of trait‐based approaches to predict species redistribution under climate change has been called into question. We develop the perspective, supported by evidence, that trait variation, if used carefully can have high potential utility, but that past analyses have in many cases failed to identify an explanatory value for traits by not fully embracing the complexity of species range shifts. First, we discuss the relevant theory linking species traits to range shift processes at the leading (expansion) and trailing (contraction) edges of species distributions and highlight the need to clarify the mechanistic basis of trait‐based approaches. Second, we provide a brief overview of range shift–trait studies and identify new opportunities for trait integration that consider range‐specific processes and intraspecific variability. Third, we explore the circumstances under which environmental and biotic context dependencies are likely to affect our ability to identify the contribution of species traits to range shift processes. Finally, we propose that revealing the role of traits in shaping species redistribution may likely require accounting for methodological variation arising from the range shift estimation process as well as addressing existing functional, geographical, and phylogenetic biases. We provide a series of considerations for more effectively integrating traits as well as extrinsic and methodological factors into species redistribution research. Together, these analytical approaches promise stronger mechanistic and predictive understanding that can help society mitigate and adapt to the effects of climate change on biodiversity.
occTest: An integrated approach for quality control of species occurrence data
Authors: Serra‐Diaz Josep M. , Borderieux Jeremy , Maitner Brian , Boonman Coline C. F. , Park Daniel , Guo Wen‐Yong , Callebaut Arnaud , Enquist Brian J. , Svenning Jens‐C. , Merow Cory 
Published: April 2024

AbstractAimSpecies occurrence data are valuable information that enables one to estimate geographical distributions, characterize niches and their evolution, and guide spatial conservation planning. Rapid increases in species occurrence data stem from increasing digitization and aggregation efforts, and citizen science initiatives. However, persistent quality issues in occurrence data can impact the accuracy of scientific findings, underscoring the importance of filtering erroneous occurrence records in biodiversity analyses.InnovationWe introduce an R package, occTest, that synthesizes a growing open‐source ecosystem of biodiversity cleaning workflows to prepare occurrence data for different modelling applications. It offers a structured set of algorithms to identify potential problems with species occurrence records by employing a hierarchical organization of multiple tests. The workflow has a hierarchical structure organized in testPhases (i.e. cleaning vs. testing) that encompass different testBlocks grouping different testTypes (e.g. environmental outlier detection), which may use different testMethods (e.g. Rosner test, jacknife,etc.). Four different testBlocks characterize potential problems in geographic, environmental, human influence and temporal dimensions. Filtering and plotting functions are incorporated to facilitate the interpretation of tests. We provide examples with different data sources, with default and user‐defined parameters. Compared to other available tools and workflows, occTest offers a comprehensive suite of integrated tests, and allows multiple methods associated with each test to explore consensus among data cleaning methods. It uniquely incorporates both coordinate accuracy analysis and environmental analysis of occurrence records. Furthermore, it provides a hierarchical structure to incorporate future tests yet to be developed.Main conclusionsoccTest will help users understand the quality and quantity of data available before the start of data analysis, while also enabling users to filter data using either predefined rules or custom‐built rules. As a result, occTest can better assess each record's appropriateness for its intended application.
Usefulness of the serum creatinine/cystatin C ratio as a blood biomarker for sarcopenia components among age groups in community‐dwelling older people: The SONIC study
Authors: Fang Wen , Godai Kayo , Kabayama Mai , Akagi Yuya , Kido Michiko , Akasaka Hiroshi , Takami Yoichi , Ikebe Kazunori , Arai Yasumichi , Masui Yukie , Ishizaki Tatsuro , Yasumoto Saori , Gondo Yasuyuki , Yamamoto Koichi , Tabara Yasuharu , Kamide Kei 
Published: April 2024

AimThe serum creatinine/cystatin C ratio (CCR) or sarcopenia index is considered a useful marker of muscle mass. However, its usefulness in late‐stage older adults remains unclear. We aimed to determine the usefulness of CCR as an indicator of sarcopenia in community‐dwelling Japanese adults aged >75 years.MethodsOur study recruited participants aged 70, 80, and 90 ± 1 years during the baseline years, and included a 3‐year follow‐up in the Septuagenarians, Octogenarians, Nonagenarians, Investigation with Centenarians study. From 2015 to 2018, 955 participants were eligible: 367 in their 70s, 304 in their 80s, and 284 in their 90s. The diagnostic components of sarcopenia, including “low muscle mass, plus low muscle strength, and/or low physical performance,” were evaluated using the bioelectrical impedance analysis‐measured skeletal muscle mass index (SMI), handgrip strength, and short physical performance battery (SPPB) score, respectively, in accordance with the Asia Working Group for Sarcopenia 2019 criteria. Separate analyses were performed between each component and CCR, adjusting for sex, body mass index, and other blood biomarkers in each group.ResultsThe relationship between CCR and sarcopenia components was significant for handgrip strength (β = 0.21, 0.13, 0.19, and P < 0.0001, =0.0088, <0.0001, for the 70s, 80s, and 90s age groups, respectively); however, it was limited for SMI (β = 0.14; P = 0.0022, only for the 90s) and not significant for the SPPB score.ConclusionCCR is a limited indicator of sarcopenia in late‐stage older adults. Although its association with muscle strength was significant, its relationship with muscle mass and physical performance was less pronounced. Geriatr Gerontol Int 2024; 24: 529–536.
Consideration of Vadose Zone Moisture Dynamics in Remediation of PFAS‐Impacted Soils
Authors: Karimi Askarani Kayvan , Cook John S. , Connor John A. , Newell Charles J. 
Published: April 2024

AbstractOilfield brines are soluble, non‐volatile, and recalcitrant contaminants that are vulnerable to capillary effects and evapoconcentration and have been known to recontaminate clean fill soils emplaced after excavations. This problem may also occur for some poly‐ and perfluoroalkyl substances (PFAS) at PFAS sites. In this paper, we consider the soil moisture characteristic curve, wetting front dynamics, and the moisture redistribution cycle to understand the potential behavior of different PFAS in the vadose zone. Drawing on experience with remediation of soils impacted by brine spills, we provide recommendations on the design and implementation of soil excavation and cover remedies such as capillary barriers to prevent recontamination.
Progressive superficial siderosis despite complete remission of intracranial hypotension symptoms following epidural patching: Case report
Authors: Carroll Ian , Schievink Wouter 
Published: April 2024

AbstractSpontaneous intracranial hypotension (SIH) commonly results from ventral spinal cerebrospinal fluid (CSF) leaks and epidural patches are advocated as first‐line treatment. Complications such as superficial siderosis can arise but have previously been reported only in the context of long‐term persistent, ongoing, CSF leak and SIH. We report a case of a patient with SIH from a ventral spinal CSF leak that was treated with epidural patching and experienced complete resolution of SIH. Four years later SIH symptoms recurred, and brain magnetic resonance imaging unexpectedly showed the interval accumulation of hemosiderin pigmentation on the cerebellum and brainstem during the period when the patient was without symptoms of SIH. This case uniquely demonstrates the progression of superficial siderosis despite the apparent resolution of SIH. Our findings suggest two divergent pathophysiological outcomes from spinal ventral dural tear: (1) CSF loss causing SIH; and (2) persistent low‐level bleeding arising from the spinal dural tear leading to superficial siderosis. These divergent pathophysiologies had a discordant response to epidural patching. Epidural patching successfully treated the SIH but did not prevent the progression of superficial siderosis, indicating that some patients may require more than epidural patching despite symptom resolution. This case highlights the need for post‐treatment monitoring protocols in patients with ventral spinal CSF leaks and SIH and raises important questions about the adequacy of epidural patching in certain SIH cases arising from ventral spinal CSF leak.
The experience of shared decision‐making for people with asthma: A systematic review and metasynthesis of qualitative studies
Authors: Kang Hui‐qi , Peng Yueming , He Yuanyuan , Yang Xiufen , Su Jin , Yang Qiaohong , Luo Weixiang 
Published: April 2024

AbstractObjectivesTo identify, describe and synthesise the views and experiences of adults living with asthma regarding shared decision‐making (SDM) in the existing qualitative literatureMethodsWe conducted a comprehensive search of 10 databases (list databases) from inception until September 2023. Screening was performed according to inclusion criteria. Tools from the Joanna Briggs lnstitute were utilised for the purposes of data extraction and synthesis in this study. The data extraction process in this study employed the Capability, Opportunity and Motivation Model of Behaviour (COM‐B model) as a framework, and a pragmatic meta‐aggregative approach was employed to synthesise the collected results.ResultsNineteen studies were included in the metasynthesis. Three synthesised themes were identified: the capability of people living with asthma, the opportunities of people living with asthma in SDM, and the motivation of the people living with asthma in SDM.ConclusionsWe have identified specific factors influencing people living with asthma engaging in SDM. The findings of this study can serve as a basis for the implementation of SDM in people living with asthma and provide insights for the development of their SDM training programs. The ConQual score for the synthesised findings was rated as low. To enhance confidence, future studies should address dependability and credibility factors.Practice ImplicationsThis review contemplates the implementation of SDM from the perspective of people living with asthma, with the aim of providing patient‐centred services for them. The results of this review can benefit the implementation of SDM and facilitate information sharing. It offers guidance for SDM skills training among adults living with asthma, fosters a better doctor–patient relationship and facilitates consensus in treatment decisions, thereby enabling personalised and tailored medical care.Patient or Public ContributionThree nursing graduate students participated in the data extraction and integration process, with two students having extensive clinical experience that provided valuable insights for the integration.
Shaping research for people living with co‐existing mental and physical health conditions: A research priority setting initiative from the United Kingdom
Authors: Taylor Olivia , Newbronner Elizabeth , Cooke Helen , Walker Lauren , Wadman Ruth 
Published: April 2024

AbstractIntroductionThose with severe and enduring mental ill health are at greater risk of long‐term physical health conditions and have a reduced life expectancy as a result. Multiple factors compound this health inequality, and the need for setting research priorities in this area is highlighted with physical and mental healthcare services being separate, and limited multimorbidity research.MethodsThe aim of this exercise was to work in partnership with healthcare professionals and carers, family, friends and individuals with lived experience of both mental and physical health conditions, to set research priorities to help people with mental health conditions to look after their physical health. The exercise was guided by the James Lind Alliance approach. For this, a steering group was set up, two surveys were completed and a final priority workshop was conducted.ResultsThis priority setting exercise guided by people's needs and lived experience has produced a set of well‐defined research topics. Initially, 555 research questions were suggested in the first survey, which were refined to 54 questions for the second survey. A priority setting workshop was then conducted to get the final 10 priorities.ConclusionsTaking these topics forward to improve services and treatment for both mental and physical ill health may in turn improve physical health and lessen the reduced life expectancy of those living with mental ill health.Patient or Public ContributionThis work was completed in collaboration with people who have lived experience of mental ill health and physical health conditions, as well as carers, family and friends. Their contribution has been significant for this work from piloting surveys, amending language used and educating the researchers and contributing to this paper. The initial work was completed with a steering group and continued with surveys and workshops.
Unlocking the potential of sorghum: a comparative study exploring the physical, chemical, technological, and digestibility properties of new cultivars from Mozambique
Authors: Mabureza Aldo Jose , Narita Isabela Mendes Pacheco , Frasson Sabrina Feksa , Van Schie Bart , Vanier Nathan Levien , Elias Moacir Cardoso , Colussi Rosana 
Published: April 2024

SummaryThis study aimed to evaluate the physical, chemical, technological, and digestibility of three sorghum cultivars (Mucuvea, Otela, and Tocole) and compared them to a Local variety (criola) grown in Mozambique. Thousand‐grain weight, hectolitre weight, and colour were determined in the grains. Proximal composition, antinutritional factors, starch and protein digestibility, and antioxidant activity were analysed in flours; starch was isolated and characterised by its physical, chemical, morphological, and digestibility properties. The grain characterisation varied among the cultivars compared to the Local variety, but not between the cultivars. The cultivars showed lower amounts of protein, lipids, free phenolics, tannin, soluble protein, digestibility of starch flour and proteins, and higher flavonoids and antioxidant activity. A high content of amylose (~37%), glycaemic index (~82), Tp (64.83 °C), and resistant starch (57.73%) were verified in all cultivars. The peak viscosity of Local variety was higher (343.25 RVU) than the other cultivars, while the breakdown (less than 106.54 RVU) of the cultivars was significantly lower than Local (182.25 RVU). Such data suggest great potential use of Local variety in bakery products and the cultivars in products that require greater viscosity. These results will reinforce the research, production, and manufacture of new products in Africa, especially in sorghum‐producing countries.
Characterisation of psyllium husk with different sizes and their effects on gel properties of surimi
Authors: Zhu Shichen , Jin Yan , Wei Chuanshuang , Yang Qing , Wei Zhengpeng , Song Hua , Liu Shulai , Ding Yicheng , Zhou Xuxia 
Published: April 2024

SummaryPsyllium husk has attracted increasing interest in food applications due to its well‐known health benefits. Size reduction is an important mechanical operation used to tailor the functional properties of materials. In this study, the physicochemical properties and colloidal stability of the PHPs with different sizes were characterised by particle size distribution, zeta potential, Fourier transform infrared (FT‐IR) and X‐ray photoelectron spectroscopy (XPS). Furthermore, the size effects of psyllium husk on surimi gel properties were investigated in terms of the gel strength, water‐holding capacity (WHC) and water distribution. The results found that milling changed the monosaccharide composition and increased the diffraction intensity of psyllium husk powder (PHP) without disrupting the β‐glycosidic linkages. Furthermore, the XPS results suggested that smaller samples showed more exposed surface functional groups. The PHP3 group with average size of d (0.5) = 259.50 μm exhibited the highest absolute zeta potential. The viscosity and zeta potential of PHP correlated positively and negatively with the gel strength of surimi, respectively. Moreover, the micronized PHP also contributed to the increase in immobilised water content and uniform water distribution in surimi gels. The high viscosity and colloidal stability of psyllium husk (PHP3 group) contributed to the improved mechanical properties of surimi gels. This work provides a valuable reference for the potential use of psyllium husk in quality improvement of surimi products through size control.
Incorporation of brewer's spent grain into plant‐based meat analogues: benefits to physical and nutritional quality
Authors: da Silva Aurenice Maria Mota , Lima Mariah Almeida , Koksel Filiz , Sato Ana Carla Kawazoe 
Published: April 2024

SummaryBrewer's spent grain (BSG) is the major by‐product of brewery industry. Due to being rich in dietary fibres and proteins, it has great potential to be upcycled in plant‐based foods and contribute to the sustainability of our food system. This study investigated the incorporation of BSG at different concentrations to soy protein‐based high‐moisture meat analogues (HMMAs). Colour, textural properties, and macrostructure of the resulting HMMAs were examined. In vitro protein digestibility of selected HMMAs was also measured. At 15% BSG incorporation level, the presence of BSG favoured texturisation and lowered the hardness of HMMAs. However, higher BSG incorporation levels impaired the formation of a fibrous structure in the HMMAs that mimics the mouthfeel of animal‐based products. When extrusion feed moisture increased from 60% to 70%, the hardness and chewiness of the HMMAs decreased from 223.18 and 188.94 N to 127.80 and 103.16 N, respectively, while the cohesiveness and springiness were not significantly affected. In general, the higher the BSG incorporation level, the darker and browner were the HMMAs. IVPD of HMMAs varied from 74.58% to 76.15% and was higher in the HMMAs containing BSG, indicating that BSG complements soy protein digestibility. BSG incorporation may improve the texture of HMMAs and contribute to the intake of dietary fibre.