bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attenuation of Colitis-Induced Visceral Hypersensitivity and Pain by Selective Silencing of TRPV1-Expressing Fibers in Rat Colon
Authors: Mazor Yoav , Engelmayer Nurit , Nashashibi Halla , Rottenfußer Lisa , Lev Shaya , Binshtok Alexander M 
Published: March 2024

Abstract

Background
Transient receptor potential vanilloid 1 (TRPV1) cation channels, expressed on nociceptors, are well established as key contributors to abdominal pain in inflammatory bowel disease (IBD). Previous attempts at blocking these channels have been riddled with side effects. Here, we propose a novel treatment strategy, utilizing the large pore of TRPV1 channels as a drug delivery system to selectively inhibit visceral nociceptors.

Methods
We induced colitis in rats using intrarectal dinitrobenzene sulfonic acid. Visceral hypersensitivity, spontaneous pain, and responsiveness of the hind paws to noxious heat stimuli were examined before and after the intrarectal application of membrane-impermeable sodium channel blocker (QX-314) alone or together with TRPV1 channel activators or blockers.

Results
Intrarectal co-application of QX-314 with TRPV1 channel activator capsaicin significantly inhibited colitis-induced gut hypersensitivity. Furthermore, in the model of colitis, but not in naïve rats, QX-314 alone was sufficient to reverse gut hypersensitivity. The blockade of TRPV1 channels prevented this effect of QX-314. Finally, applying QX-314 alone to the inflamed gut inhibited colitis-induced ongoing pain.

Conclusions
Selective silencing of gut nociceptors by a membrane-impermeable sodium channel blocker entering via exogenously or endogenously activated TRPV1 channels diminishes IBD-induced gut hypersensitivity. The lack of effect on naïve rats suggests a selective analgesic effect in the inflamed gut. Our results suggest that in the colitis model, TRPV1 channels are tonically active. Furthermore, our results emphasize the role of TRPV1-expressing nociceptive fibers in colitis-induced pain. These findings provide proof of concept for using charged activity blockers for the blockade of IBD-associated abdominal pain.
Awake sternal fixation; comparison of technical details and early results with sternal fixation methods performed via general anaesthesia
Authors: Gurz Selcuk , Dost Burhan , Pirzirenli Mehmet Gokhan , Buyukkarabacak Yasemin , Taslak Sengul Aysen , Kaya Cengiz , Temel Necmiye Gul , Ozdemir Emine , Basoglu Ahmet 
Published: March 2024

Abstract

OBJECTIVES
Isolated sternal fractures are rare pathologies that rarely require surgical fixation. Although different fixation techniques are used, it is routinely performed under general anaesthesia. In our study, we aimed to share the details of the awake sternal fixation technique performed in our clinic and to compare the early results with sternal fixation methods performed under general anaesthesia.

METHODS
Between January 2009 and January 2023, 129 patients who were diagnosed with sternal fracture and who underwent investigations and follow-up in our clinic were evaluated retrospectively. Thirteen patients who underwent surgical fixation for isolated sternal fracture were included in the study. Patients were categorized according to fixation and anaesthetic technique; group 1: fixation with steel wire under general anaesthesia (n = 4), group 2: fixation with titanium plate–screw under general anaesthesia (n = 4) and group 3: fixation with awake titanium plate–screw with parasternal intercostal plane block (n = 5). Demographics, surgical indication, radiological findings, surgical incision, surgical time and hospital stay were statistically compared.

RESULTS
The mean age of the patients included in the study was 55.15 ± 15.01 years and 84.6% (n = 11) were male. The most common reason for fixation was displaced fracture (53.8%). Fixation surgery was performed due to pain in 30.8% (n = 4) and non-union in 15.4% (n = 2) of the fractures. The mean duration of surgery were 98.75 ± 16.52, 77.5 ± 35 and 41 ± 14.74 min, respectively. Duration of surgery was significantly lower in group 3 compared to the other groups (P = 0.012). The hospital stay duration for group 1 was 6 days, group 2 was 4 days and group 3 was 1 day. A notable difference was observed among all groups (P = 0.019).

CONCLUSIONS
Awake sternal fixation technique with titanium plate–screw system under superficial parasternal intercostal plane block is an easy and effective method for surgical treatment of isolated sternal fractures. This technique showed a direct positive effect on the duration of surgery and hospital stay.
Risk of infective endocarditis after hybrid melody mitral valve replacement in infants: the French experience
Authors: Padovani Paul , Jalal Zakaria , Fouilloux Virginie , Benbrik Nadir , Grunenwald Céline , Thambo Jean-Benoit , Aldebert Philippe , Tagorti Maha , Roubertie François , Baron Olivier , Ovaert Caroline , Ly Mohamedou , Baruteau Alban-Elouen 
Published: March 2024

Abstract

OBJECTIVES
Surgical management of mitral valve disease is challenging in infants &lt;1 year old. We aimed at reviewing the French experience with Melody mitral valve replacement in critically ill infants.

METHODS
A retrospective cohort study reporting the French experience with Melody mitral valve replacement.

RESULTS
Seven symptomatic infants [complete atrioventricular septal defect (n = 4, Down syndrome: n = 3), hammock valve (n = 3)] underwent Melody mitral valve replacement [age: 3 months (28 days to 8 months), weight: 4.3 kg (3.2–6.4 kg)] because of severe mitral valve regurgitation (6) or mixed valve disease (1) and 14 mm (11–16 mm) mitral valve annulus. In 2 patients whose valve was felt irreparable, Melody mitral valve replacement was performed straightaway. The others underwent 2 (1–3) previous attempts of valve repair; 3 were on extracorporeal membrane oxygenation. Melody mitral valve replacement led to competent valve and low gradient [3 mmHg, (1–4 mmHg)]. One patient died 3 days post-implant from extracorporeal membrane oxygenation-related stroke. Of the 6 discharged home patients, 3 (50%) were readmitted for a definite diagnosis (1) or high suspicion (2) of infective endocarditis, of which 2 died. Over the follow-up, 1 underwent balloon expansions of the valve at 9- and 16-months post-implant, and mechanical mitral valve replacement at 2 years; another is currently planned for transcatheter Melody valve dilation.

CONCLUSIONS
Melody mitral valve replacement may be considered in selected infants with small mitral valve annulus as an alternative to mechanical mitral valve replacement. Our experience highlights a high-risk of late infective endocarditis that deserves further consideration.
Protective Role of NS1-Specific Antibodies in the Immune Response to Dengue Virus Through Antibody-Dependent Cellular Cytotoxicity
Authors: Sanchez-Vargas Luis A , Mathew Anuja , Salje Henrik , Sousa David , Casale Nicole A , Farmer Aaron , Buddhari Darunee , Anderson Kathryn , Iamsirithaworn Sopon , Kaewhiran Surachai , Friberg Heather , Currier Jeffrey R , Rothman Alan L 
Published: March 2024

Abstract

Background
Dengue virus (DENV) nonstructural protein 1 (NS1) has multiple functions within infected cells, on the cell surface, and in secreted form, and is highly immunogenic. Immunity from previous DENV infections is known to exert both positive and negative effects on subsequent DENV infections, but the contribution of NS1-specific antibodies to these effects is incompletely understood.

Methods
We investigated the functions of NS1-specific antibodies and their significance in DENV infection. We analyzed plasma samples collected in a prospective cohort study prior to symptomatic or subclinical secondary DENV infection. We measured binding to purified recombinant NS1 protein and to NS1-expressing CEM cells, antibody-mediated natural killer (NK) cell activation by plate-bound NS1 protein, and antibody-dependent cellular cytotoxicity (ADCC) of NS1-expressing target cells.

Results
We found that antibody responses to NS1 were highly serotype cross-reactive and that subjects who experienced subclinical DENV infection had significantly higher antibody responses to NS1 in preinfection plasma than subjects who experienced symptomatic infection. We observed strong positive correlations between antibody binding and NK activation.

Conclusions
These findings demonstrate the involvement of NS1-specific antibodies in ADCC and provide evidence for a protective effect of NS1-specific antibodies in secondary DENV infection.
Application of a user experience design approach for an EHR-based clinical decision support system
Authors: Gao Emily , Radpavar Ilana , Clark Emma J , Ryan Gery W , Ross Mindy K 
Published: March 2024

Abstract

Objective
We applied a user experience (UX) design approach to clinical decision support (CDS) tool development for the specific use case of pediatric asthma. Our objective was to understand physicians’ workflows, decision-making processes, barriers (ie, pain points), and facilitators to increase usability of the tool.

Materials and methods
We used a mixed-methods approach with semi-structured interviews and surveys. The coded interviews were synthesized into physician-user journey maps (ie, visualization of a process to accomplish goals) and personas (ie, user types). Interviews were conducted via video. We developed physician journey maps and user personas informed by their goals, systems interactions, and experiences with pediatric asthma management.

Results
The physician end-user personas identified were: efficiency, relationship, and learning. Features of a potential asthma CDS tool sought varied by physician practice type and persona. It was important to the physician end-user that the asthma CDS tool demonstrate value by lowering workflow friction (ie, difficulty or obstacles), improving the environment surrounding physicians and patients, and using it as a teaching tool. Customizability versus standardization were important considerations for uptake.

Discussion
Different values and motivations of physicians influence their use and interaction with the EHR and CDS tools. These different perspectives can be captured by applying a UX design approach to the development process. For example, with the importance of customizability, one approach may be to build a core module with variations depending on end-user preference.

Conclusion
A UX approach can drive design to help understand physician-users and meet their needs; ultimately with the goal of increased uptake.
Parental betaine supplementation promotes gosling growth with epigenetic modulation of IGF gene family in the liver
Authors: Ma Shuai , Wang Yan , Chen Liang , Wang Wenzheng , Zhuang Xinjuan , Liu Yuelong , Zhao Ruqian 
Published: March 2024

Abstract
Betaine is widely used as a feed additive in the chicken industry to promote laying performance and growth performance, yet it is unknown whether betaine can be used in geese to improve the laying performance of goose breeders and the growth traits of offspring goslings. In this study, laying goose breeders at 39 wk of age were fed basal (Control, CON) or betaine-supplemented diets at low (2.5 g/kg, LBT) or high (5 g/kg, HBT) levels for 7 wk, and the breeder eggs laid in the last week were collected for incubation. Offspring goslings were examined at 35 and 63 d of age. The laying rate tended to be increased (P = 0.065), and the feed efficiency of the breeders was improved by betaine supplementation, while the average daily gain of the offspring goslings was significantly increased (P &lt; 0.05). Concentrations of insulin-like growth factor 2 (IGF-2) in serum and liver were significantly increased in the HBT group (P &lt; 0.05), with age-dependent alterations of serum T3 levels. Concurrently, hepatic mRNA expression of the IGF gene family was significantly increased in goslings derived from betaine-treated breeders (P &lt; 0.05). A higher ratio of proliferating cell nuclear antigen (PCNA)-immunopositive nuclei was found in the liver sections of the HBT group, which was confirmed by significantly upregulated hepatic expression of PCNA mRNA and protein (P &lt; 0.05). Moreover, hepatic expression of thyroxine deiodinase type 1 (Dio1) and thyroid hormone receptor β (TRβ) was also significantly upregulated in goslings of the HBT group (P &lt; 0.05). These changes were associated with significantly higher levels of global DNA 5-mC methylation, together with increased expression of methyl transfer genes (P &lt; 0.05), including betaine-homocysteine methyltransferase (BHMT), glycine N-methyltransferase (GNMT), and DNA (cytosine-5-)-methyltransferase 1 (DNMT1). The promoter regions of IGF-2 genes, as well as the predicted TRβ binding site on the IGF-2 gene, were significantly hypomethylated (P &lt; 0.05). These results indicate that gosling growth can be improved by dietary betaine supplementation in goose breeders via epigenetic modulation of the IGF gene family, especially IGF-2, in the liver.
Management of Outpatient Pediatric Burns at a Pediatric Burn Center
Authors: Bostancı Süleyman Arif , Demir Sabri , Ertürk Ahmet , Demirtaş Gökhan , Öztorun Can İhsan , Güney Doğuş , Demirkaya Şükrüye , Erten Elif Emel , Azılı Müjdem Nur , Şenel Emrah 
Published: March 2024

Abstract
Burn injuries are a serious emergency. Most burn injuries in children can be treated as outpatients. The aim of this study was to present the clinical and epidemiologic characteristics of pediatric burn injuries treated in our outpatient burn clinic. This study included pediatric patients treated in an outpatient burn clinic over an 8-year period. The demographic and clinical characteristics of the patients were retrospectively analyzed. The patients were divided into 4 groups according to their age groups, and the differences between the groups were investigated. Statistical analysis was performed using IBM SPSS Statistics, Version 25.0. P &lt; .05 was accepted as statistically significant. Of the total 5167 patients, 2811 (54.4%) were male. Scald burns were the most common cause of burns (81.4%). Contact burns accounted for 12.2% of all burns. There were no differences between sexes for any variable. The highest incidence was seen in children in the 2- to 5-year-old age group (28.3%). There were differences between age groups in terms of sex, burn area, burn visit, burn type, burn location, need for wound dressing under anesthesia, and need for grafting. Among the patients, 4.9% were hospitalized due to the need for wound dressing under anesthesia. However, most of the patients (95.1%) were treated as outpatients. In conclusion, the majority of pediatric burn patients can be treated as outpatients. This allows pediatric patients to complete treatment in a psychologically comfortable environment and has low complication rates. Outpatient treatment should become the standard for children who are suitable for outpatient follow-up and who have home support.
Data-driven multivariate regression-based anomaly detection and recovery of unmanned aerial vehicle flight data
Authors: Yang Lei , Li Shaobo , Li Chuanjiang , Zhu Caichao 
Published: March 2024

Abstract
Flight data anomaly detection is crucial for ensuring the safe operation of unmanned aerial vehicles (UAVs) and has been extensively studied. However, the accurate modeling and analysis of flight data is challenging due to the influence of random noise. Meanwhile, existing methods are often inadequate in parameter selection and feature extraction when dealing with large-scale and high-dimensional flight data. This paper proposes a data-driven multivariate regression-based framework considering spatio-temporal correlation for UAV flight data anomaly detection and recovery, which integrates the techniques of correlation analysis (CA), one-dimensional convolutional neural network and long short-term memory (1D CNN-LSTM), and error filtering (EF), named CA-1DCL-EF. Specifically, CA is first performed on original UAV flight data to select parameters with correlation to reduce the model input and avoid the negative impact of irrelevant parameters on the model. Next, a regression model based on 1D CNN-LSTM is designed to fully extract the spatio-temporal features of UAV flight data and realize parameter mapping. Then, to overcome the effect of random noise, a filtering technique is introduced to smooth the errors to improve the anomaly detection performance. Finally, two common anomaly types are injected into real UAV flight datasets to verify the effectiveness of the proposed method.
Sensitivity analysis for the generalization of experimental results
Authors: Huang Melody Y 
Published: March 2024

Abstract
Randomized controlled trials (RCT’s) allow researchers to estimate causal effects in an experimental sample with minimal identifying assumptions. However, to generalize or transport a causal effect from an RCT to a target population, researchers must adjust for a set of treatment effect moderators. In practice, it is impossible to know whether the set of moderators has been properly accounted for. I propose a two parameter sensitivity analysis for generalizing or transporting experimental results using weighted estimators. The contributions in the article are threefold. First, I show that the sensitivity parameters are scale-invariant and standardized, and introduce an estimation approach for researchers to account for both bias in their estimates from omitting a moderator, as well as potential changes to their inference. Second, I propose several tools researchers can use to perform sensitivity analysis: (1) numerical measures to summarize the uncertainty in an estimated effect to omitted moderators; (2) graphical summary tools to visualize the sensitivity in estimated effects; and (3) a formal benchmarking approach for researchers to estimate potential sensitivity parameter values using existing data. Finally, I demonstrate that the proposed framework can be easily extended to the class of doubly robust, augmented weighted estimators.
Seeing and turbulence profile simulations over complex terrain at the Thai National Observatory using a chemistry-coupled regional forecasting model
Authors: Macatangay Ronald , Rattanasoon Somsawatt , Butterley Timothy , Bran Sherin Hassan , Sonkaew Thiranan , Sukaum Boonchoo , Sookjai Donrudee , Panya Mana , Supasri Titaporn 
Published: March 2024

Abstract
This study utilized advanced numerical simulations with the Weather Research and Forecasting model coupled with Chemistry (WRF-Chem) to predict anticipated astronomical seeing conditions at the Thai National Observatory (TNO). The study evaluated the effects of both gas-phase and aerosol-phase chemical processes in the Earth’s atmosphere, along with the impact of spatial and temporal resolution on model performance. These simulations were validated against measurements from the Differential Image Motion Monitor (DIMM) and the Slope Detection and Ranging (SLODAR) technique. Due to the inherent temporal variability of the DIMM observations, a 24-h moving average window was applied to both DIMM data and WRF-Chem model outputs. This reduced the percentage root-mean-square error in the comparison between the two data sets from 23 per cent to 11 per cent and increased the correlation coefficient from 0.21 to 0.59. Chemistry played a minor role during the study period, contributing 3.49 per cent to astronomical seeing. However, it did affect the model’s accuracy. Additionally, the study revealed that higher spatial and temporal resolution simulations did not necessarily improve the model’s accuracy. When compared to SLODAR observations of the refractive index structure constant (Cn2dh), the simulations captured altitude variations within ±25 per cent above 5 km and 25–50 per cent below 5 km. Dome seeing also played a role, contributing to around 90 per cent or more in the lowest altitude layer. The results emphasized the significance of seeing predictions in providing valuable insights into complex atmospheric phenomena and how to mitigate the effects of atmospheric turbulence on telescopes.
A dependable distance estimator to black hole low-mass X-ray binaries
Authors: Abdulghani Y , Lohfink A M , Chauhan J 
Published: March 2024

ABSTRACT
Black hole low-mass X-ray binaries (BH-LMXBs) are excellent observational laboratories for studying many open questions in accretion physics. However, determining the physical properties of BH-LMXBs necessitates knowing their distances. With the increased discovery rate of BH-LMXBs, many canonical methods cannot produce accurate distance estimates at the desired pace. In this study, we develop a versatile statistical framework to obtain robust distance estimates soon after discovery. Our framework builds on previous methods where the soft spectral state and the soft-to-hard spectral state transitions, typically present in an outbursting BH-LMXB, are used to place constraints on mass and distance. We further develop the traditional framework by incorporating general relativistic corrections, accounting for spectral/physical parameter uncertainties, and employing assumptions grounded in current theoretical and observational knowledge. We tested our framework by analysing a sample of 50 BH-LMXB sources using X-ray spectral data from the Swift/XRT, MAXI/GSC, and RXTE/PCA missions. By modelling their spectra, we applied our framework to 26 sources from the 50. Comparison of our estimated distances to previous distance estimates indicates that our findings are dependable and in agreement with the accurate estimates obtained through parallax and H i absorption methods. Investigating the accuracy of our constraints, we have found that estimates obtained using both the soft and transition spectral information have a median uncertainty (1σ) of 20 per cent, while estimates obtained using only the soft spectral state spectrum have a median uncertainty (1σ) of around 50 per cent. Furthermore, we have found no instrument-specific biases.
Defining the contribution of microRNA-specific Argonautes with slicer capability in animals
Authors: Pal Anisha , Vasudevan Vaishnav , Houle François , Lantin Michael , Maniates Katherine A , Huberdeau Miguel Quévillon , Abbott Allison L , Simard Martin J 
Published: March 2024

Abstract
microRNAs regulate gene expression through interaction with an Argonaute protein. While some members of this protein family retain an enzymatic activity capable of cleaving RNA molecules complementary to Argonaute-bound small RNAs, the role of the slicer residues in the canonical microRNA pathway is still unclear in animals. To address this, we created Caenorhabditis elegans strains with mutated slicer residues in the endogenous ALG-1 and ALG-2, the only two slicing Argonautes essential for the miRNA pathway in this animal model. We observe that the mutation in ALG-1 and ALG-2 catalytic residues affects overall animal fitness and causes phenotypes reminiscent of miRNA defects only when grown and maintained at restrictive temperature. Furthermore, the analysis of global miRNA expression shows that the slicer residues of ALG-1 and ALG-2 contribute differentially to regulate the level of specific subsets of miRNAs in young adults. We also demonstrate that altering the catalytic tetrad of those miRNA-specific Argonautes does not result in any defect in the production of canonical miRNAs. Together, these data support that the slicer residues of miRNA-specific Argonautes contribute to maintaining levels of a set of miRNAs for optimal viability and fitness in animals particularly exposed to specific growing conditions.