bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osmotic Properties of Gluten
Authors: Bot Arjen , de Bruijne Dick W. 
Published: June 2007

ABSTRACTA model for dough is proposed in which the distribution of water between hydrated gluten and starch paste explains a number of practical observations such as 1) the extreme sensitivity of the consistency of dough to the amount of water in the recipe, and 2) the fact that working of the material results in an increase in consistency. The model assumes dough to be a composite material consisting of a starch paste and gluten filaments. During kneading, the starch granule paste in dough dries to become a phase with a yield stress as a result of the uptake of water by the stretching gluten filaments. This study focused on one particular aspect of this model: the osmotic properties of gluten during stretching. The results suggest that gluten can be hydrated more efficiently in the stretched state than in an unstretched conformation. Gluten hydration tends to change slowly over a period of weeks, which is accompanied by water expulsion or uptake, depending on the osmotic properties of the solvent. The rate of change does not seem to depend very much on pH and osmotic pressure for the current experimental conditions. The level of hydration of relaxed gluten depends strongly on pH, as expected. The experiments allow the construction of an osmotic pressure versus gluten concentration diagram over the range 4.6 < pH < 5.8. The level of hydration of the gluten is consistent with the proposed model for dough.
Chemometric Localization Approach to NIR Measurement of Apparent Amylose Content of Ground Wheat
Authors: Wesley I. J. , Osborne B. G. , Anderssen R. S. , Delwiche S. R. , Graybosch R. A. 
Published: June 2007

ABSTRACTThe development of new wheat cultivars that target specific end‐uses, such as low or zero amylose contents of partially waxy and waxy wheats, has become a modern focus of wheat breeding. But for efficient and cost‐effective breeding, inexpensive and high‐throughput quality testing procedures, such as near infrared (NIR) spectroscopy, are required. The genetic nature of a set of wheat lines, which included waxy to nonwaxy cultivars, results in a bimodal distribution of amylose contents that presents some special challenges for the formulation of stable NIR calibrations for this property. The obvious and intuitive solution lies in the use of some form of localization procedure and we explored three localization algorithms in comparison with the default partial least squares. Localization with respect to the waxy (zero amylose) cultivars resulted in a modified partial least squares calibration with a standard error of prediction of 0.16%. The results establish unambiguously that there are advantages in performing a suitable localization to achieve a reliable NIR calibration and prediction. The accuracy of the method can also be enhanced by application of an appropriate resampling strategy. In addition, there are advantages in performing a suitable localization to achieve a reliable NIR calibration‐prediction. It resolves the issue of how to utilize the bimodal distribution of apparent amylose values. The best results are obtained when the localization is performed simultaneously with respect to the sample property under investigation and the NIR spectra. The key problem with the measurement of amylose is the laboratory reference method which, in reality, only measures the apparent amylose content of the wheat. As a direct consequence, the measurements of amylose have such a large error that traditional calibration‐prediction procedures generate unacceptable results. To resolve this difficulty, a statistically based resampling strategy is proposed as a method of identifying samples where there is a large error in the reference measurement.
Protein and Starch Properties of Some Tetraploid Wheats
Authors: Sissons M. J. , Batey I. L. 
Published: June 2007

ABSTRACTThe tetraploid relatives (subspecies) of commercial durum wheat (Triticum turgidum L. subsp. turgidum conv. durum (Desf.) MacKey) offer a source of economically useful genes for the genetic improvement of durum cultivars. Thirty‐two accessions, representing five different subspecies: var. durum (13 accessions), polonicum (7), persicum (3), turanicum (5), and turgidum (4) were grown at Tamworth, Australia, in 1997 and 1999. These accessions were compared with three durum cultivars: Wollaroi and Kamilaroi (in both years) and Yallaroi (in 1998 only). In this study, the glutenin subunit composition and molecular weight distribution, together with starch properties of these accessions, were studied. A much wider range in both the glutenin subunit composition and the starch RVA paste viscosities and gelatinization profiles were found in the accessions compared with the cultivated durum wheats. Most of the accessions had lower gluten strength and the presence of poor quality LMW alleles, and low proportions of unextractable polymeric protein could explain this. For starch, RVA peak viscosity correlated strongly with cooking loss of pasta, the only significant correlation between starch properties and measured aspects of pasta quality.
Fermented Sorghum as a Functional Ingredient in Composite Breads
Authors: Hugo Leda F. , Rooney Lloyd W. , Taylor John R. N. 
Published: June 2007

ABSTRACTWhole sorghum flour was fermented (a five‐day natural lactic acid fermentation) and dried under forced draught at 60°C, and evaluated for its effect on sorghum and wheat composite bread quality. In comparison with unfermented sorghum flour, fermentation decreased the flour pH from 6.2 to 3.4, decreased total starch and water‐soluble proteins, and increased enzyme‐susceptible starch, total protein, and the in vitro protein digestibility (IVPD). Fermentation and drying did not decrease the pasting temperature of sorghum flour, but slightly increased its peak and final viscosity. In comparison with composite bread dough containing unfermented sorghum flour, fermented and dried sorghum flour decreased the pH of the dough from 5.8 to 4.9, increased bread volume by ≈4%, improved crumb structure, and slightly decreased crumb firmness. IVPD of the composite bread was also improved. Mixing wet fermented sorghum flour directly with wheat flour (sourdough‐type process) further increased loaf volume and weight and reduced crumb firmness, and simplified the breadmaking process. It appears that the low pH of fermented sorghum flour inactivated amylases and increased the viscosity of sorghum flour, thus improving the gas‐holding capacity of sorghum and wheat composite dough. Fermentation of sorghum flour, particularly in a sourdough breadmaking process, appears to have considerable potential for increasing sorghum utilization in bread.
Contribution of Specific Flour Components to Water Vapor Adsorption Properties of Wheat Flours
Authors: Roman‐Gutierrez Alma Delia , Mabille Frédéric , Guilbert Stéphane , Cuq Bernard 
Published: June 2007

ABSTRACTThe dynamic water vapor adsorption properties were determined for two wheat flours (hard wheat flour and soft wheat flour) and compared with those of flour components (starch, damaged starch, gluten, water‐soluble pentosans, and water‐insoluble pentosans). Water vapor adsorption rates were determined from the changes in sample mass as a function of time during hydration after a step increase in relative humidity (rh). It was not possible to significantly discriminate the selected products by initial rates of adsorption (5.1 × 10‐2 to 6.4 × 10‐2 g/100 g of dry matter/min), except the water‐insoluble pentosans that were characterized by high values of adsorption rates (14 × 10‐2 g/100 g of dry matter/min). Changes in initial relative humidity conditions and %rh step sizes induced significant changes in adsorption rates. Calculations of apparent water diffusion coefficients were done using a derived form of Fick's law for polydisperse spherical particles. Apparent water diffusion coefficients (at 25°C and 60% rh) were estimated between 2.19 × 10‐15 and 3.72 × 10‐15 m2/sec for the selected wheat flours. Water‐insoluble pentosans are characterized by the highest values of diffusion coefficients (1.53 × 10‐13 m2/sec) when compared with the other wheat components. The calculated values of apparent water diffusion coefficient were discussed in regard to experimental conditions.
Nutritional and Economic Implications of Protein Variance and Application of Statistical Process Control in the Chinese Feed Industry
Authors: Yan Zhichun , Herrman Timothy J. , Loughin Thomas , Featherstone Allen M. , Feng Dingyaun 
Published: June 2007

ABSTRACTThe adoption of Statistical Process Control (SPC) in the Chinese feed industry to reduce finished feed protein and moisture content variation can improve feed quality and profitability. Two studies were conducted to characterize protein variation in the Chinese feed industry. Study I involved four participating feed mills where we collected three samples per bag from four bags per lot of soybean meal, rapeseed meal, fish meal, corn gluten meal, meat & bone meal, and cottonseed meal. We analyzed these ingredients for protein and moisture content. Cottonseed meal and meat & bone meal displayed the highest protein variability between bags and within bags compared with other protein ingredients. We found no significant difference for within‐bag protein variance of the other feed ingredients; however, soybean meal had a significantly lower between‐bag variance (0.04%). Study II assessed the effect of adopting SPC. Five feed mills provided data for one year of finished feed protein and moisture content before and after adopting SPC. Three of the five feed mills experienced a significant (P < 0.10) reduction (0.20, 0.52, and 0.70%) in the absolute finished feed protein variation after adopting SPC. The two feed mills that did not display a significant reduction in absolute protein variation were already manufacturing feed with a low (<0.4% protein) absolute deviation from the target. These data demonstrate that feed mill managers can control finished feed variability through a number of management techniques including SPC.
Sequential Extraction Processing of High‐Oil Corn
Authors: Hojilla‐Evangelista Mila P. , Johnson Lawrence A. 
Published: June 2007

ABSTRACTThe Sequential Extraction Process (SEP), a process that uses ethanol to fractionate corn into high‐value co‐products when producing fuel ethanol, was evaluated using high‐oil corn (HOC). Oil and protein recoveries, ethanol‐drying capability, and oil and protein properties were compared with those produced from normal soft dent corn (SDC) using SEP. Moisture adsorption capacities (≈24 g of water/kg of corn) and oil recoveries (>95%) were nearly identical for both corn types. However, oil yield from HOC (7.1 g of oil/100 g of corn) was 65% more than that of SDC (4.3 g of oil/100 g of corn). HOC crude oil was less red and contained lower free fatty acid and phosphatide contents than did SDC oil. HOC zein contained higher crude protein content (86% db) than did SDC zein (79% db). The freeze‐dried glutelin‐rich fractions (GRF) from both types of corn contained >90% (db) crude protein, which meet the criteria of protein isolates. SEP GRF protein isolates had >85% protein soluble in water at pH ≥ 7. Both GRF protein isolates from HOC and SDC were heat‐stable, had good emulsifying capacities, and produced highly stable emulsions. They also had substantial foaming capacities, but the foam from HOC GRF protein isolate was significantly more stable than the foam from SDC GRF protein isolate. SEP is a suitable process for recovering oil and protein products from HOC corn when producing fuel ethanol and, if competitively priced, HOC is a preferred feedstock to SDC because of higher yields and qualities of valuable co‐products when using HOC.
Agronomic and Quality Performance of Progeny Lines Derived from Spring Wheat by Durum Wheat Crosses
Authors: Lanning S. P. , Habernicht D. , Martin J. M. , Sherman J. D. , Fischer A. , Talbert L. E. 
Published: June 2007

ABSTRACTHard red spring wheat (Triticum aestivum L. subsp. aestivum) and durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn) have both been selected for dryland yield potential and high grain protein, although end uses of flour are different. For this study, 14 tetraploid and 10 hexaploid lines were derived from crosses between tetraploid durum wheat and hexaploid hard red spring wheat. Our objective was to determine the impact of genetic exchange between the two classes on agronomic and quality attributes of derived progeny lines. Yield potential of both the tetraploid and hexaploid progeny was lower than the parental types. Polyphenol oxidase levels were higher by almost twofold in the hexaploid group. The tetraploid progeny group had better noodle color than did the hexaploid group. Bread quality of hexaploid group was superior, with loaf volume 362 cm3 higher than that of tetraploid progeny group. In general, the presence of durum wheat genes in the derived hexaploid lines had little impact on most quality traits. The presence or absence of the D genome tended to be the major influence on end use quality traits. Our results suggest that genetic exchange between the two groups has a small effect on end use quality, but low yield potential in the progeny suggests obtaining desirable genetic combinations for plant improvement may be challenging.
Resistance to α‐Amylase Digestion in Four Native High‐Amylose Maize Starches
Authors: Evans Annette , Thompson Donald B. 
Published: June 2007

ABSTRACTNative and processed high‐amylose maize starch (HAMS) is an important source of resistant starch (RS). The objectives of this work were to use an in vitro procedure to estimate the RS content of native granules from a series of ae‐containing HAMS genotypes, and to examine the nature of the α‐amylase resistant starch (ARS). By the method of Englyst et al (1992), RS for ae V, ae VII, ae su2, and ae du were estimated to be 66.0, 69.5, 69.5, and 40.6%, respectively. By transmission electron microscopy, most of the residual granules from ae V, ae VII, and ae su2 showed little evidence of digestion. Partially digested granules had a radial digestion pattern in the interior and an enzyme‐resistant layer near the surface. Size and chain‐length profile of constituents of ARS were similar to those of the native HAMS (unlike type 3 RS), consistent with complete hydrolysis in susceptible granule regions. Between crossed polarizers, many iodine‐stained native and residual HAMS granules had blue centers and pink exteriors, which may be due to a difference in orientation of the amylose‐iodine complexes in the exterior. Four granule color types were observed for ae du, differing in enzyme resistance. The high‐enzyme resistance of native HAMS granules may result from altered granule organization, which appears to vary among and within granules from ae‐containing genotypes.
Gelatinization Properties of Starches from Three Successive Generations of Six Exotic Corn Lines Grown in Two Locations
Authors: Ji Y. , Pollak L. M. , Duvick S. , Seetharaman K. , Dixon P. M. , White P. J. 
Published: June 2007

ABSTRACTThe objectives of this research were to evaluate the intra‐ and interpopulation variability in gelatinization properties of starches from exotic corn lines and their derivatives when grown 1) during two successive years in the same location; and 2) in both temperate and tropical environments. Six novel exotic corn lines (two 100% exotic and four 25% exotic derived from a breeding cross developed by crossing an exotic hybrid with Corn Belt lines) were selected for this research because their starches have significantly different (and potentially useful) thermal properties from those found in starch from normal Corn Belt corn. The Sn (n = 3 for 25% exotic lines and n = 1 for 100% exotic lines) generations of the six exotic lines were self‐pollinated and grown in the winter nursery in Puerto Rico. Two successive generations (Sn+1 and Sn+2) of lines selected for low onset of gelatinization temperature were self‐pollinated and grown in the same environment near Ames, IA. To evaluate the effect of environment, the Sn+2 generation also was self‐pollinated and grown in the winter nursery in Puerto Rico. Thermal properties of starches from 10 single kernels from each line were analyzed by using differential scanning calorimetry (DSC) at a ratio of 4 mg of dry starch to 8 mg of distilled water. After subsequent generations, the differences in DSC gelatinization properties between selected kernels within each progeny line narrowed, suggesting increased homogeneity of starch structural properties within each line. Unusual thermal properties were fixed in some progeny lines. Environmental factors also affected the thermal properties of starch and a significant interaction between environment and genotype was observed. These results suggest that introgression of adapted germplasm with useful genes from exotic corn would increase the available genetic variability for starch functionality and allow the development of hybrids with important value‐added traits.
A Maltose Biosensor for Determining Gelatinized Starch in Processed Cereal Foods
Authors: Marconi Emanuele , Messia Maria Cristina , Palleschi Giuseppe , Cubadda Raimondo 
Published: June 2007

ABSTRACTA reliable method for the quantitative determination of gelatinized starch in processed cereal foods was developed. It consists of an electrochemical biosensor based on amyloglucosidase and glucose oxidase enzymes co‐immobilized on a Pt electrode surface, and a third enzyme, α‐amylase, added in solution. Analytical parameters such as time, temperature, and enzyme units were optimized. The degree of starch gelatinization was determined in different processed cereal foods using the biosensor method and the results were commensurate to those obtained with the reference method. The biosensor methods showed good accuracy (r2 = 0.9629; relative error <12%) and comparable precision (RSD <5%). This electrochemical system is rapid, reliable, inexpensive, user‐friendly for unskilled operators, and can be a valid alternative to the methods traditionally used for gelatinized starch analysis.
Reduced Amylose Effects on Bread and White Salted Noodle Quality
Authors: Martin John M. , Talbert Luther E. , Habernicht Debra K. , Lanning Susan P. , Sherman Jamie D. , Carlson Gregg , Giroux Michael J. 
Published: June 2007

ABSTRACTAmylose content in wheat endosperm is controlled by three Wx loci, and the proportion of amylose decreases with successive accumulation of Wx null alleles at the three loci. The proportion of amylose is believed to influence end‐use quality of bread and Asian noodles. The objectives of this study were to determine influence of the allelic difference at Wx‐B1 locus on bread quality, bread firmness, and white salted noodle texture in a spring wheat cross segregating for the Wx‐B1 locus and in a set of advanced spring wheat breeding lines differing in allelic state at the Wx‐ B1 locus. In addition, we examined the relationship between amylose content and flour swelling properties on bread and noodle traits. Fifty‐four recombinant inbred lines of hard white spring wheat plus parents were grown in replicated trials in two years, and 31 cultivars and breeding lines of hard spring wheat were grown in two locations. Bread and white salted noodles were processed from these trials. The presence of the Wx‐B1 null allele reduced amylose content by 2.4% in a recombinant inbred population and 4.3% in a survey of advanced breeding lines and cultivars compared with the normal. The reduced amylose was accompanied by an average increase in flour swelling power (FSP) for the Wx‐B1 null group of 0.8 g/g for the cross progeny and 2.3 g/g for the cultivar survey group. The Wx‐B1 allelic difference did not affect flour protein in cross progeny where the allelic difference was not confounded with genetic background. Bread from the Wx‐B1 null groups on average had increased loaf volume and was softer than the normal group for the cross progeny and cultivar survey group. The Wx‐B1 allelic difference altered white salted noodle texture, most notably noodle springiness and cohesiveness where the Wx‐B1 null groups was more springy and more cohesive than the normal groups for both sets of genetic materials. Flour protein was more highly related to loaf volume than were FSP or amylose. Both flour protein and FSP were positively related to noodle textural traits, but especially noodle springiness and cohesiveness.
Selected Functional Properties of Extruded Starch Acetate and Natural Fibers Foams
Authors: Guan J. , Fang Q. , Hanna M. A. 
Published: June 2007

ABSTRACTResponse surface methodology was employed to study the functional properties of starch acetate foams blended with 0, 7.5, and 15% wood, oat, and cellulose fibers. The blends were extruded with 14, 17, and 20% ethanol as a blowing agent, using a twin‐screw extruder with 160°C barrel temperature and 225 rpm screw speed. Physical characteristics of the extrudates including radial expansion ratio, unit density, and bulk density; and mechanical properties including unit spring index and bulk spring index were determined. Scanning electron micrographs were taken to observe foam cell textures. Higher fiber content resulted in lower radial expansion. Ethanol content had a positive effect on foam expansion. Higher expansion was obtained in starch acetate‐cellulose foams because better starch acetate‐fiber matrix was formed. Mechanical properties increased with higher fiber and ethanol contents. Micrographs showed that uniform cell structures were associated with better mechanical properties.
Biochemical Characterization of γ‐75 k Secalins of Rye II. Disulfide Bonds
Authors: Gellrich Claudia , Schieberle Peter , Wieser Herbert 
Published: June 2007

ABSTRACTAggregated γ‐75k secalins were isolated by preparative reversed‐phase (RP) HPLC of the prolamin fraction of rye flour Danko and were digested with thermolysin. The resulting peptides were preseparated by gel permeation HPLC into eight fractions (G1–G8). Peptides that were linked by disulfide bonds (cystine peptides) were identified by means of differential chromatography (RP‐HPLC before and after the reduction of disulfide bonds). The cystine peptides present in fractions G3–G7 were isolated by preparative RP‐HPLC and characterized by sequence analysis and, in parts, by mass spectrometry. Accordingly, the eight cysteine residues of the C‐terminal domain of γ‐75k secalins were linked in the same positions as the intramolecular disulfide bonds of γ‐gliadins of wheat. The cysteine residue located at position 12 of the N‐terminal domain and characteristic for γ‐75k secalins was linked by an intermolecular disulfide bond with a corresponding residue of the same protein type. This cysteine residue is likely to be responsible for the aggregative nature of γ‐75k secalins similar to a cysteine residue in the N‐terminal domain of LMW subunits of wheat glutenin. In contrast to LMW subunits of glutenin, γ‐75k secalins do not possess an additional cysteine residue in the C‐terminal domain that forms a second intermolecular disulfide bond. Therefore, the polymerization of γ‐75k secalins is limited and the formation of large gluten‐like aggregates of rye storage proteins is restricted.