bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the outcomes of biliary-enteric reconstruction in robotic radical resection of hilar cholangiocarcinoma: a single-centre propensity score matching analysis
Authors: Liu Jie , Dou Changwei , Chen Jian , Lu Yi , Liang Lei , Wei Fangqiang , Zhang Chengwu 
Published: April 2024

Abstract
Background
Although robotic radical resection for hilar cholangiocarcinoma(HCCA) has been reported in few cases in some large hepatobiliary centers, biliary-enteric reconstruction (BER) is still a critical step which restricts the success of the operation. This study aimed to evaluate the feasibility and quality of BER in robotic and laparoscopic radical resection of HCCA and propose technical recommendations.
Methods
A retrospective study was conducted on patients with HCCA who underwent minimally invasive radical resection at Zhejiang Provincial People’s Hospital from January 2016 to July 2023. A 1:2 propensity score matching (PSM), which is widely used to reduce selection bias, was performed to evaluate the surgical outcomes, especially BER-related data between robotic and laparoscopic surgery.
Results
46 HCCA patients were enrolled. After PSM at a ratio of 1:2, 10 and 20 patients were assigned to the robotic-assisted and laparoscopic groups, respectively. And the baseline characteristics of two groups were generally well balanced. The average time of liver resection in the robotic group was longer than that in the laparoscopic group( 139.5 ± 38.8 vs 108.1 ± 35.8, P = 0.036), but the former had less intraoperative blood loss(200(50–500) vs 310(100–850)), even though there was no statistical difference(P = 0.109). The biliary residuals number was 2.6 ± 1.3 and 2.7 ± 1.2(P = 0.795); the number of anastomoses were both 1.6 ± 0.7 in two groups(P = 0.965); the time of BER was 38.4 ± 13.6 and 59.1 ± 25.5 min(P = 0.024), 9.9 ± 2.8% and 15.4 ± 4.8% of the total operation time(P = 0.001). Although postoperative bile leakage incidence in laparoscopic group(40.0%)was higher than that in robotic group(10.0%), there was no significant difference between two groups(P = 0.204); 6.7 ± 4.4 and 12.1 ± 11.7 days for tube drawing(P = 0.019); anastomosis stenosis and calculus rate was 10.0% and 30.0% (P = 0.372), 0.0% and 15%(P = 0.532), respectively. Neither group had a hemorrhage or bile leakage-related death.
Conclusion
Robotic radical resection for HCCA may offer the comparable perioperative outcomes as conventional laparoscopic procedure, and tend to be associated with more advantages in terms of anastomosis time and quality, we are optimistic with its wide application in future with the improvement of surgical techniques and experience.
Experiences of residents in a therapeutic community model of drug addiction treatment in a mental hospital in Ghana
Authors: LINDSAY KWEKU EWUSIE ORLEANS , WOOD SANDRA FREDA , Abraham Susanna Aba , Kwamena Aidoo Ebenezer Ato 
Published: April 2024

Abstract

Background:
In Ghana, the use of illegal drugs and alcohol has been classified as endemic. To manage this menace, the Therapeutic Community model for Substance Used Disorders was introduced in Ghana about a decade ago as an alternative to other existing strategies. However, the effectiveness of this treatment modality for drug addiction in Panteng Mental Hospital and Accra Psychiatric Hospital has not been scientifically studied.
Objective:
This study, therefore, sought to explore the experiences of drug addicts or residents enrolled in the Therapeutic Community programme toward recovery from drug addiction.
Method:
This research employed an explorative-descriptive design. Participants (N=20) were enrolled purposively sampled and recruited into the study. Face-to-face interviews were conducted using a semi-structured guide and data were analyzed using Colaizzi’s descriptive phenomenology data analysis process.
Results:
Two main themes emerged from the data: “Why I stayed” (Motivation), and “All things are new” (Life transformation). The study pointed out that residents or participants mainly expressed positive experiences in the programme, including experiencing a sense of belongingness, enhanced self-esteem/self-worth, and most importantly, developing hope of a life free of addiction.
Conclusion:
In conclusion, the study found that participants in the Therapeutic Community (TC) program generally had positive experiences and believed in its effectiveness for recovery. Factors such as a relaxed environment, supportive staff, and interactions with other residents contributed to their positive experience. The study discovered that motivation, a conducive environment for care, structured program activities, and relationships among residents were key factors that motivated participants to stay and actively participate in the therapeutic community program. These factors fostered hope and a sense of belonging, ultimately contributing to their recovery journey. However, participants expressed the need for self-actualization, which they hoped to achieve after completing the program and reintegrating into the community.
Assessment of disordered eating attitude and associated factors among female undergraduate students at Arba Minch University, Southern Ethiopia 2023
Authors: Damtie Addisalem , Meried Melkamu , Getie Asmare , Belayhun Yosef 
Published: April 2024

Abstract

Introduction
eating disorders significantly damage physical health and disturb psychosocial operation. The global burden of anorexia nervosa and bulimia nervosa among young females was estimated to account 1.9 million disability–adjusted life years. Even though earlier identification of disordered eating attitude is important to prevent more sever forms of eating disorder, there is scarce evidence at university students in Ethiopia. Therefore, conducting this study has paramount importance in recognition of these unfavourable attitudes and to take remedial action accordingly.
Objective
To assess disordered eating attitude and associated factors among Arba Minch university female students, Arba Minch, Southern Ethiopia, 2023
Methods
an institution based cross-sectional study was employed at Arba Minch University from January 1/2023 to April1/ 2023. A total of 600 study participants were included in the study. A simple random sampling technique was used to select participants. The data were collected using self-administered questionnaire, entered using Epi-Info software and exported to SPSS version 20 for analysis. A multivariable binary logistic regression analysis was used to identify factors associated with eating disorder and variables with p-value < 0.05 were considered as statistically significant. Model fitness was checked using Hosmer and Lemeshow Goodness of fit test. The strength of association between the outcome variable and independent variables was measured by using adjusted odds ratio with the corresponding 95% confidence interval.
Result
The result of this study shows that the prevalence of disordered eating attitude among undergraduate female students of Arba Minch University is 11.5% (95% CI: 9.0-14.3). Specialization or department (AOR = 2.27, 95% CI; 1.06–4.86) and Body mass index (AOR = 5.83, 95% CI; 2.34–14.52) were found to be predictors for disordered eating attitude.
Conclusion and recommendation
the prevalence shouldn’t be under estimated and it’s possible to say many female students in Arba Minch University are susceptible for eating disorder. Body mass index and academic specialization or department were significantly associated with disordered eating attitude and can be considered as predictors of disordered eating attitude.
Prevalence of Malaria and Associated Factors among Children under 5 Years Following Mass Net Campaigns and Indoor Residual Spraying in Amolatar District-Northern Uganda: Cross-Sectional Study
Authors: Orech Sam , Opollo Marc Sam , Ocen Francis , Akello Ruth Anne 
Published: April 2024

Abstract

Background: Mass LLIN distribution campaigns and indoor residual spraying (IRS) have been identified by the WHO as effective approaches for malaria prevention and control.
Despite the scale-up of interventions towards the control and elimination of malaria, it is still endemic in 91 countries worldwide, with 3.3 billion people at risk of developing it. In 2015, 212 million malaria cases occurred globally, resulting in 429,000 deaths, 92% of which were in Africa. In 2019, 409,000 people died of malaria—mostly vulnerable children in sub-Saharan Africa.
Methods: A community-based, cross-sectional research design using a quantitative data collection method was used. A semi-structured questionnaire was used. Bivariate and multivariate logistic regression analyses were performed to identify factors associated with the prevalence of malaria among <5 years.
Results: The intended respondents (238) were interviewed, and up to 44.1% (105/238) of the children tested positive for malaria. According to the multivariate analysis, living with a married caretaker (AOR=2.54, 95% CI=1.23-5.25) had a statistically significant association with malaria incidence, and poor perceptions of LLIN use had 68% increased odds of contracting malaria. However, children who stayed far from water bodies (AOR=0.07, 95% CI=0.01-0.56) had reduced odds of developing malaria.
Conclusions and recommendations: Malaria remains the major public health problem among children under 5 years of age in the Amolatar district. Living with married caretakers, having poor perceptions of LLIN use and living near water bodies were risk factors for malaria. DHTs to strengthen malaria prevention and control strategies among children <5 years, health information dissemination about eliminating stagnating water bodies, and addressing poor perceptions of mothers of children <5 years.
Characterizing the Biopotency of Truncated Transgene Variants in rAAV8 viral vectors: Essential Considerations for Gene Therapy Applications
Authors: Fiedler Christian , Lengler Johannes , Gruber Bernadette , Scheindel Marcus , Büngener Carsten , Mittergradnegger Dominik , Bendik Marian , Kraus Barbara , Bort Juan A. Hernandez 
Published: April 2024

Abstract

Background
The production of recombinant Adeno-Associated Virus (rAAV) vectors for gene therapy applications is a critical process, especially when dealing with large transgenes that challenge the 4.7kb capacity limit of rAAV. We aimed to investigate the variances in recombinant rAAV samples post-ultracentrifugation produced in HEK293, focusing on those rAAV8s with either single-stranded (ss) or self-complementary (sc) DNA. Special attention was given to partially filled variants, which might reflect incomplete transgene structures in the manufacturing of rAAV8-FVIII and rAAV8-FIX. A variety of analytical techniques were applied to thoroughly examine these fractions.
Results
Our data revealed that the so-called "partially empty" capsids, which were presumed to have very low biological activity, actually showed biopotency levels and a significant contribution to the total vector preparation similar to that of the complete rAAV8 fractions. Observations extended to other rAAV8 variants in our lab, including those carrying ssDNA and scDNA for human coagulation factors IX Padua and FVIII. These findings highlight the need to reevaluate the criteria used to differentiate between full and empty rAAV capsids in the purification process.
Conclusions
Our approach was instrumental in discovering that fractions containing low full/empty ratios exhibited high biopotency. This information enabled us to pinpoint the location of truncated forms in the collected samples, thereby allowing us to choose the most suitable fractions for pooling. In addition, we hypothesize that an amount of these truncated forms might reconstitute into complete genes in host cells and therefore we propose a reconstitution model, challenging traditional views on the necessity of only full rAAV capsids for gene therapy treatments.
Alpine viper in changing climate: thermal ecology and prospects of a cold-adapted reptile in the warming Mediterranean
Authors: Mizsei Edvárd , Radovics Dávid , Rák Gergő , Budai Mátyás , Bancsik Barnabás , Szabolcs Márton , Sos Tibor , Lengyel Szabolcs 
Published: April 2024

Abstract

Climate change represents a fundamental threat to species adapted to cold alpine environments. We studied the thermal ecology of the endangered Greek meadow viper (Vipera graeca), an endemic venomous snake of fragmented alpine-subalpine meadows above 1600 m of the Pindos mountain range in Greece and Albania. We measured preferred body temperature in artificial thermal gradient, field body temperatures and the availability of environmental temperatures in five populations encompassing the entire geographic range of the species. We found that the preferred body temperature (Tp) differed between the northernmost and the southernmost populations and increased with female body size but did not depend on sex or the gravidity status of femalesTp increased with latitude but was unaffected by the phylogenetic position of the populations. We also found high accuracy of thermoregulation in V. graeca populations and variation in the thermal quality of habitats throughout the range. The overall effectiveness of thermoregulation was high, indicating that V. graeca successfully achieves its target temperatures and exploits the thermal landscape. Current climatic conditions limit the activity period by an estimated 1278 hours per year, which is expected to increase considerably under future climate change. Restricted time available for thermoregulation, foraging and reproduction will represent a serious threat to the fitness of individuals and the persistence of populations in addition to habitat loss due to mining, tourism or skiing and habitat degradation due to overgrazing in the shrinking mountaintop habitats of V. graeca.
Osteonecrosis of the Jaw (ONJ) Associated with Bevacizumab: A Case Series from the FDA Adverse Event Reporting System (FAERS) Database
Authors: Ahdi Hardeep , Wichelmann T. Adam , Pandravada Sasirekha , Ehrenpreis Eli 
Published: April 2024

Abstract

Introduction:
Osteonecrosis of the jaw (ONJ) is a rare but serious adverse drug reaction (ADR) historically associated with denosumab and bisphosphonate therapy. Studies have demonstrated an association between ONJ and bevacizumab, a VEGF inhibitor now used for a variety of malignancies. This study reviewed cases of bevacizumab associated ONJ reported to the FDA Adverse Event Reporting System (FAERS) database
Methods:
The FAERS database was searched for all reported cases of ONJ from 2010 to 2021. A dataset was created for bevacizumab-associated ONJ and demographics were summarized. Only adults (age 18 and older) and reports from healthcare professionals were included for analysis. Duplicate cases were removed. Cases lacking information on patient age or gender were excluded from review. A subgroup analysis was performed for subjects receiving bevacizumab without prior or concomitant denosumab or bisphosphonate therapy.
Results:
19,670 cases of ONJ were reported to the FAERS database between 2010 to 2021 with approximately half (9,556) containing complete patient demographic data. 146 cases (1.5% of the total) of bevacizumab-associated ONJ were identified. 96 (65.8%) of the patients were female and the average age was 60.9 years +/- 9.3 years. There were 34 cases without prior or concomitant bisphosphonate or denosumab therapy. Male gender occurred in 65% (22 of 34) of these cases and average age was 61 years +/- 12.0. Table/Figure 1 depicts clinical indications for each of these 34 cases. Gastrointestinal malignancies (i.e., colon cancer and esophageal cancer) were present in 6/34 (17.6%) cases without prior denosumab/bisphosphonate therapy, 5 patients of breast cancer (14.7%) and 5 cases of prostate cancer were also noted(14.7%).
Discussion:
146 cases of bevacizumab-associated ONJ have been reported to the FAERS database and 23% of these cases did not involve medications known to cause ONJ. GI malignancy was the most common primary malignancy for reports of bevacizumab-associated ONJ. Clinicians should consider ONJ as a potential adverse effect in patients with malignancies that are being treated with bevacizumab.
Binding Affinity Screening of Polyphenolic Compounds in Stachys Affinis extract (SAE) for their Potential Antioxidant and Anti- inflammatory Effects
Authors: Kim Hun Hwan , Jeong Se Hyo , Park Min Yeong , Bhosale Pritam Bhangwan , Abusaliya Abuyaseer , Lee Sang Joon , Heo Jeong Doo , Kim Hyun Wook , Seong Je Kyung , Kim Dong Il , Park Kwang Il , Kim Gon Sup 
Published: April 2024

Abstract

Free radical is a marker in various inflammatory diseases. The antioxidant effect protects us from this damage, which also plays an essential role in preventing inflammation. Inflammation protects the body from biological stimuli, and pro-inflammatory mediators are negatively affected in the immune system. Inflammation caused by LPS is an endotoxin found in the outer membrane of Gram-negative bacteria, which induces immune cells to produce inflammatory cytokines such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Based on this, the antioxidant and anti-inflammatory effects of plant extracts were investigated. First, the main phenolic compounds for the five peaks obtained from Stachys Affinis Extract (SAE) were identified. The antioxidant effect of each phenolic compound was confirmed through HPLC analysis before and after the competitive binding reaction between DPPH and the extract. Afterward, the anti-inflammatory effect of each phenolic compound was confirmed through competitive binding between COX2 and the extract in HPLC analysis. Lastly, the anti-inflammatory effect of SAE was confirmed through in vitro experiments and also confirmed in terms of structural binding through molecular docking. This study confirmed that phenolic compounds in SAE extract have potential antioxidant and anti-inflammatory effects, and may provide information for primary screening of medicinal plants.
Association of birth weight, childhood obesity, and age at menarche with ovarian function: an integrated Mendelian randomization study and bioinformatics analysis
Authors: Dang Chunxiao , Liu Pengfei , Liu Jinxing , Yu Xiao 
Published: April 2024

Abstract
Objective Observational studies have shown that birth weight, childhood obesity and age at menarche are associated with ovarian function, but there is still some controversy.
Methods We mainly uses the inverse variance weighted (IVW) method for analysis, conducting heterogeneity, horizontal pleiotropy and MR Steiger test to evaluate the robustness of the results and the strength of causal relationships. Finally, preliminary bioinformatics analysis was conducted to explore the underlying biological mechanisms.
Results Causal associations were found between childhood obesity, age at menarche and ovarian function, with a positive association between childhood obesity and ovarian dysfunction and a negative association between age at menarche and ovarian function. A total of 10 hub genes were identified, which are interconnected in an interaction network and play a role in the synthesis and secretion of lipids and parathyroid hormone.
Conclusions Our study genetically confirms the causal association between childhood obesity, age at menarche and ovarian function; childhood obesity increases the risk of primary ovarian failure, and the later the age at menarche, the lower the risk of ovarian failure, which may be related to alterations in metabolic pathways such as intracellular receptors-mediated alterations in lipids and hormones.
The study predicts and validates the overburden load and stability behaviors of top slab structures Based on hybrid intelligent modeling
Authors: Chen Qingfa , Liu Chenyang 
Published: April 2024

Abstract

It is important to determine the exact value of the overburden load on the tunnel roof to assess the stability of the roof, ho wever, th is parameter is poorly constrained. Based on this, we use the hybrid intelligence approach to deal with the nonlinear relationship between multiple parameters and the overlying load. Firstly, a multi parameter correlation visualization analysis is performed to determine two hidden layers (E 1 , E 2 ), and four parameters with the most influence on the overlying load were selected, which will be used as sample input parameters. Secondly, the top plate stability prediction is achieved by converting the input and output to a single thermal code, and then adding parameter thresholds that were continuously adjusted with training . This completes the structure of ANN IHHO model . Finally, the model is trained based on data driven by displacement inverse analysis and numerical experiments. The model prediction accuracy was then validated using a confusion matrix form . The validation results show that the trained ANN IHHO model has good accuracy and can predict the top plate stability under the condition of considering four parameters The proposed model provides a new way of thinking to determine the overburden load and stability of the top plate . This study proposes many new research ideas in the process of developing the model : First, the m ulti parameter correlation visualization analysis can be used in studies to assess the degree of correlation between parameters and results, also between parameters and parameters And most importantly, the visualization analysis can intuitively display the correlation degree . the introduction of displacement inverse analysis as a data acquisition method into the ANN model has proven to be feasible, and the method has the advantage of being low cost and easy to implement ; Third, the c onfusion matrix also shows good adaptability in verifying model prediction accuracy, especially for prediction models of rock stability The method proposed in this study to develop the model is also adapted to the prediction of other rock mechanical parameters.
Genomic landscape in the human vaginal microbiome links to host geographics and genetics
Authors: Chen Chen , Jie Zhuye , Liang Weiting , Ding Qiuxia , Tong Xin , Zhang Yunhong , Chen Na , Li Shenghui , Liu Xiaomin , Gao Hongqin , Huang Xincheng , Zhang Zhe , Liu Na , Xie Zhangwei , Wang Xiaman , Qi Le , Li Yumei , Xiao Liang , Zhang Shaoqiao , Jin Xin , Xu Xun , Yang Huanming , Wang Jian , Zhao Fangqing , Jia Huijue , Zhang Tao , Hao Lilan , Zhu Lan 
Published: April 2024

Abstract
The vaginal microbiota is essential for women’s health, yet its genomic diversity and host relevance remains incompletely characterized. Here we established a Chinese cohort with 10,281 vaginal metagenomes. We developed an extensive catalog of vaginal microbial genomes (VMG) by integrating 6,979 in-house metagenomes with 1,817 publicly available metagenomes and over 1,000 bacterial isolates. This catalog comprised 46,906 genomes from 913 prokaryotic species and 3,763 viral populations, with 23.8% of prokaryotes and 75.1% of viruses being not found in public reference databases. Leveraging VMG, we identified substantial intraspecies genomic and functional variations within the vaginal microbiome that display geographic specificity. Notably, this included the novel bacterium CAISGS01(ID u199), unique to Chinese samples with marked biosynthetic capabilities, as well as BVAB1, which exhibited distinct regional genomic variations in pathogenic potential. Moreover, by utilizing genome-resolved microbial profiles from the VMG, we conducted a metagenome-genome-wide association study involving 6,893 individuals, and identified 7 vaginal microbial taxa whose abundances were significantly associated with 31 host genomic loci, including a notable correlation between novel bacterium Prevotella (ID u35) and loci on 22p11.2 (beta = 1.51, p = 6.826 × 10− 38). These associations were consistently and robustly replicated across three independent cohorts. In summary, our research provides a vital reference for future studies on the genotype-phenotype interplay within the human vaginal microbiome.