bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultra-fast genetically encoded sensor for precise real-time monitoring of physiological and pathophysiological peroxide dynamics
Authors: Berndt Andre , Lee Justin , Won Woojin , Kimball Kandace , Neiswanger Carlie , Schattauer Selena , Wang Yihan , Yeboah Fred , Ruiz Micaela , Evitts Kira , Rappleye Michael , Bremner Samantha , Chun Changho , Smith Netta , Mack David , Young Jessica , Lee C. Justin , Chavkin Charles 
Published: March 2024

Abstract
Hydrogen Peroxide (H2O2) is a central oxidant in redox biology due to its pleiotropic role in physiology and pathology. However, real-time monitoring of H2O2 in living cells and tissues remains a challenge. We address this gap with the development of an optogenetic hydRogen perOxide Sensor (oROS), leveraging the bacterial peroxide binding domain OxyR. Previously engineered OxyR-based fluorescent peroxide sensors lack the necessary sensitivity and response speed for effective real-time monitoring. By structurally redesigning the fusion of Escherichia coli (E. coli) ecOxyR with a circularly permutated green fluorescent protein (cpGFP), we created a novel, green-fluorescent peroxide sensor oROS-G. oROS-G exhibits high sensitivity and fast on-and-off kinetics, ideal for monitoring intracellular H2O2 dynamics. We successfully tracked real-time transient and steady-state H2O2 levels in diverse biological systems, including human stem cell-derived neurons and cardiomyocytes, primary neurons and astrocytes, and mouse brain ex vivo and in vivo. These applications demonstrate oROS's capabilities to monitor H2O2 as a secondary response to pharmacologically induced oxidative stress and when adapting to varying metabolic stress. We showcased the increased oxidative stress in astrocytes via Aβ-putriscine-MAOB axis, highlighting the sensor’s relevance in validating neurodegenerative disease models. Lastly, we demonstrated acute opioid-induced generation of H2O2 signal in vivo which highlights redox-based mechanisms of GPCR regulation. oROS is a versatile tool, offering a window into the dynamic landscape of H2O2 signaling. This advancement paves the way for a deeper understanding of redox physiology, with significant implications for understanding diseases associated with oxidative stress, such as cancer, neurodegenerative, and cardiovascular diseases.
Shear Stress-Induced Pre-Cytokinetic Block: A New Cellular Response Revealed by an Innovative Shear Stress Generator
Authors: Beresova Lucie , Vitecek Jan , Protivánková Iva , Dudka Michal , Chroma Katarina , Skrott Zdenek , Buchtova Tereza , Bartek Jiri , Mistrik Martin 
Published: March 2024

Abstract

During metastasis, cancer cells navigate through harsh conditions, including various mechanical forces in the bloodstream, highlighting the need to understand the impact of mechanical and shear stresses on cancer cells. To overcome the current methodological limitations of such research, here we present a new device that replicates similar conditions by applying shear stress on cultured cells. The device provides a less complex, easily accessible alternative to traditional microfluidics while generating fluid shear stress values comparable to those in human veins and capillaries. The device allows analyses of large cell numbers in standard cell culture flasks and incubators. Using this device to explore the shear stress-induced responses of various human cell lines, we discovered a previously unknown, reversible pre-cytokinetic block occurring in cells that lose anchorage during mitosis and are kept under constant shear stress. Notably, some cancer cell lines appear to bypass this unorthodox cell-cycle block, suggesting its role as a safety checkpoint to restrict the proliferation of cancer cells in the bloodstream and their overall spreading potential. These findings provide new insights into the diverse responses of normal and cancer cells to shear stress and highlight the potential of our technology for research on circulating tumor cells and metastatic spread.
Comparative structural insights and functional analysis for the distinct unbound states of Human AGO proteins
Authors: Kakoulidis Panos , Theotoki Eleni , Pantazopoulou Vasiliki , Vlachos Ioannis , Emiris Ioannis , Stravopodis Dimitrios , Anastasiadou Ema 
Published: March 2024

Abstract
The four human Argonaute (AGO) proteins, essential in RNA interference and gene regulation, exhibit high sequence and structural similarity, yet differ functionally. Our molecular dynamics simulations revealed that while AGO proteins adopt similar open-close states, each one displays distinct local conformations, leading to unique interdomain distances and intramolecular interactions. We found that GW182/ZSWIM8 interaction sites, catalytic/pseudo-catalytic tetrads and long common protein subsequences conserve their molecular movement with minute differences but have varying solvent accessibility per AGO. We observed diverse stability patterns at the post-transcriptional sites of the AGOs, except for AGO4. Combining simulation data with large datasets of experimental structures and AlphaFold’s predictions, we identified proteins with gene sequence and protein structure similarities that operate in the mitosis pathway and share mitosis-related interactors and miRNA targets. Additionally, we suggest a zinc ion binding function in AGO proteins, predicting potential binding sites and detecting structurally similar proteins with the same function.
Critical Yield Components for Achieving High Grain Yield in Ratoon Rice
Authors: He Hui , Song Linqiong , Wang Weiqin , Zheng Huabin , Tang Qiyuan 
Published: March 2024

Abstract

Ratoon rice is considered an eco-friendly and resource-efficient method for rice cultivation, providing innovative strategies to mitigate the global food crisis. To clarify the critical yield components for achieving high grain yield in ratoon rice, data from 136 widely cultivated rice cultivars were collected through a six-year field experiment. The study analyzed the correlations between yield components and yields for both the main season and the ratoon season, indicating that main yields vary between 5.9 and 10.9 t. ha-1, exhibiting a highly significant positive correlation with spikelets per panicle; ratoon yields range from 1.8 to 7.1 t. ha-1, showing a highly significant positive correlation with panicles per m², grain filling rate, and 1000-grain weight. Path analysis reveals that, in terms of contributing to ratoon yields, the grain filling rate is the most influential component, followed by panicles per m², and 1000-grain weight. Therefore, by selecting large-panicle cultivars in the main season and enhancing panicles per m², grain filling rate, and grain weight in the ratoon season, high annual yields in ratoon rice can be realized.
Numerical Analysis of Turbulent Flow around an Irregularly Shaped Airfoil
Authors: Ahmed Tausif , Bangalee Md. Zavid Iqbal , Hossain S. M. Chapal , Hossain Muhammad Sajjad 
Published: March 2024

Abstract
An airfoil is the shape of a wing. Simply put, it is the cross-section of any lift and drag-producing body. For an airplane to take flight, the most important part is played by the wings. The interaction between air and the wings of an aircraft can be tricky as there are several parameters at work. As stated above, an airfoil is a cross-section of a wing and, therefore, two-dimensional. Analyzing a two-dimensional body is both time and resource-efficient. The objective of this thesis is to study the impact and determine the factors that govern the flow around a modified airfoil in turbulence. In extreme situations, such as a tropical storm, the wing of an airfoil might get damaged and result in an accident. This work will further demonstrate the traits of the airfoil in such a situation. The forces acting on an airfoil like lift, drag, and the changes in momentum will all be discussed in the present study.
Risk Estimation of Surface Water Pollution in Vam Co Tay River Based on Remote Sensing Data and Multi-criteria Decision Analysis Methods
Authors: Vo Trung Hung , Nguyen Hien Than , Nguyen Thi Thuy Hang , Le Trong Dieu Hien 
Published: March 2024

Abstract
Satellite remote sensing (SRS) is a technique that can provide effective method on surface water quality assessment at large spatial scales studies. The analysis research involves: (1) analysis of changes in surface water quality in the Vam Co Tay River, Long An province, Vietnam in the period 2015–2020, (2) select a model to estimate water quality assessment index from remote sensing data based on Bayesian Model Averaging - BMA; and (3) quantitative assessment of surface water pollution risks in the study area. The results show that the predictive coefficients of determination (R2) for water quality (BOD5, COD, and TSS) are higher than 0.70 for all three parameters. In particular, the upstream of ​​Vam Co Tay river with "very high risk level" in 2015 tended to decrease to "high risk level" in 2020. Besides, the results also show the increasing of the risk in downstream from "low risk" in 2015 to "moderate risk" in 2020. The study demonstrated the potential of SRS for providing an overall assessment of the spatial distribution of risks associated with surface water pollution and forecasting the concentration change trends in the future, and supporting to overcome data shortages in water monitoring
Research on Preparation Technology of Brittle-resistant Asphalt Mixture Based on Wet-Dry Combined Modification Processes
Authors: Song Zhengfeng , Zheng Chuanfeng , Wei Zhiqiang , Li Hanjun , Zhao Jing 
Published: March 2024

Abstract

This study aims to reduce the brittle point temperature of asphalt binders and improve the low-temperature mechanical properties of mixtures by developing a brittle-resistant asphalt mixture preparation technology for use in cold regions. The SBS wet-modified asphalt was combined with the melted star-shaped SBS modifier through a dry method. During mixing and transportation, the high-temperature effect helped form a secondary cross-linking effect with part of the original SBS-modified asphalt. The rest was evenly dispersed in the form of tough fillers, effectively avoiding the segregation and high viscosity issues caused by the conventional high-dosage wet-modified SBS asphalt. Tests were conducted on the brittle point temperature of asphalt binders, oil–aggregate interface adhesion grade, high- and low-temperature rheological properties of asphalt binders and the high- and low-temperature performances of the mixture to analyse the scientific nature of the developed preparation technology. Results show that compared with the conventional wet-modified SBS asphalt, the brittle-resistant SBS-modified asphalt prepared based on the wet–dry method has a reduced brittle point temperature by nearly 6.6°C, maintains an adhesion grade of 5 at the oil-aggregate interface. Moreover, the m value increases by approximately 1.3 times, the low-temperature bending strain of the mixture increases by approximately 70%, and the low-temperature bending strength is enhanced by approximately 60%. These results demonstrate that the proposed brittle-resistant SBS-modified asphalt preparation technology significantly improves the low-temperature performance of the mixture whilst ensuring mixing quality, which is crucial for enhancing the service performance of asphalt pavements in cold regions.
Study on the performance of ATP grafting basalt fiber based on the plant root bionic idea and its adsorption characteristics with asphalt
Authors: YANG Chengcheng , LIU Li , LIU Zhaohui , HUANG You , PAN Houxuan , YANG Da , ZHANG Longke 
Published: March 2024

Abstract

Nano-attapulgite (ATP) is a layered silicate mineral with abundant reserves, large specific surface area, and low cost. The unique structure of ATP has attracted wide attention in the field of adsorption. In this study, a preparation technology of ATP grafting basalt fiber (BF) was proposed by chemical grafting method based on the idea of plant root bionics. The optimal preparation process of ATP grafting BF was determined through the tests of asphalt absorption performance, thermal stability performance, and segregation dispersion performance. The mechanism of ATP grafting BF was analyzed by micro-morphology, functional group changes, and elemental composition. Finally, the adsorption performance of ATP grafting BF was investigated on the basis of adsorption kinetics model and molecular dynamics simulation. The study results indicated that ATP-BFHCl had better compatibility and wettability with asphalt. The quasi-second-order kinetic equation could better fit the adsorption process of ATP-BFHCl on asphaltene, which indicated that ATP-BFHCl adsorbed asphaltene with chemisorption or ion exchange. The diffusion coefficient and diffusion activation energy of the saturate and the aromatic were larger, indicating a smaller molecular weight and faster movement, and lightweight components of asphalt are more easily adsorbed on the surface of ATP. The diffusion activation energy of asphaltene is the smallest and the reaction is the easiest to take place, which indicates that it is the first to react during temperature increase. The research results can provide a theoretical basis and technical support for BF surface treatment technology.
Parametric tuning of dynamical phase transitions
in ultracold reactions
Authors: Sinitsyn Nikolai , Sadhasivam Vijay Ganesh , Suzuki Fumika , Yan Bin 
Published: March 2024

Abstract

Advances in ultracold chemistry have led to the possibility of a coherent transformation between ultracold atoms and molecules including between completely bosonic condensates. Such transformations are enabled by the magneto-association of atoms at a Feshbach resonance which results in a passage through a quantum critical point. In this study, we show that the presence of generic interaction between the formed molecules can fundamentally alter the nature of the critical point, change the yield of the reaction and the order of the consequent phase transition. We find that the correlations introduced by this rather general interaction induce nontrivial many-body physics such as coherent oscillations between atoms and molecules, and a selective formation of squeezed molecular quantum states and quantum cat states. We provide analytical and numerical descriptions of these many-body effects, along with scaling laws for the reaction yield in both the adiabatic and non-adiabatic regimes, and highlight the potential experimental relevance in quantum sensing.
Adenoid Cystic Carcinoma of the Breast: A Case Report
Authors: Hercules Jose 
Published: March 2024

Abstract

Adenoid cystic carcinoma is a rare form of breast cancer accounting for 0.1%-1.0% of all mammary cancer malignancies. Breast cancer is characterized by an indolent clinical course and favorable prognosis, contrary to other breast cancers. Diagnostic mammogram and breast ultrasound play a pivotal role in the early detection and diagnosis of breast adenoid cystic carcinoma. Treatment may consist of lumpectomy and radiation therapy vs mastectomy alone. Although rare, late disease recurrence and metastasis have been reported in the literature; thus, long-term surveillance is of utmost importance for these patients. We will review the literature and discuss the case of a 52-year-old female who presented with a palpable lump of the right breast, which was pathologically proven to be adenoid cystic carcinoma of the breast.
Exosomal AFAP1-AS1 promotes the growth, metastasis, and glycolysis of pituitary adenoma by preventing HuR degradation
Authors: Tang Hengxin , Zhu Delong , Li Wenxiang , Zhang Guozhi , Zhang Heng , Peng Qiujiao 
Published: March 2024

Abstract

Background
Exosomal long noncoding RNAs (lncRNAs), which are highly concentrated in tumor-derived exosomes, play a crucial role in modulating cellular behaviors such as cell proliferation, metastasis, and glycolysis by facilitating intercellular communication. Here, we elucidated the role and regulatory mechanism of tumor-derived exosomal lncRNAs in pituitary adenomas (PA).
Methods
We isolated exosomes from PA cells, then performed in vitro and in vivo assays to evaluate proliferation, metastasis, and glycolysis effects. Next, we conducted RNA pull-down, RNA immunoprecipitation, co-immunoprecipitation and ubiquitination assays to investigate exosomal AFAP1-AS1’s potential downstream mechanism.
Results
Exosomes from PA cells augmented the proliferation, mobility, and glucose metabolism of PA cells. Particularly, actin filament associated protein 1 antisense RNA 1 (AFAP1-AS1) was significantly enriched in these exosomes. Furthermore, exosomal AFAP1-AS1 not only stimulated growth, migration, invasion and glucose metabolism abilities of PA cells in vitro, but also promoted tumor metastasis in vivo. Additionally, exosomal AFAP1-AS1 markedly enhanced binding affinity between Hu antigen R (HuR) and SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), resulting in HuR ubiquitination and degradation to upregulate HK2 and PKM2 expression. Moreover, HuR overexpression impaired exosomal AFAP1-AS1-mediated promotion of growth, metastasis and glycolysis effects.
Conclusions
These findings indicate that cancer-derived exosomal AFAP1-AS1 modulated SMURF1-mediated HuR ubiquitination and degradation to upregulate HK2 and PKM2 expression, thereby potentially contributing to the promotion of PA cell growth, metastasis, and glucose metabolism. Targeting the exosomal AFAP1-AS1 may be a potential strategy for the treatment of PA.
Sheffield Adaptive Patterned Electrical Stimulation (SHAPES) Therapy for Post Stroke Arm Spasticity: study protocol for a 3-arm, a partially blinded, randomised controlled trial
Authors: Ali Ali , McCarthy Avril D , Reeves Mark , Healey Jamie , Moody Louise , Adebajo Adewale , Good Tim , Dixon Simon , Baster Kathleen , Tindale Wendy , Nair Krishnan Padmakumari Sivaraman 
Published: March 2024

Abstract

Introduction
Post stroke elbow spasticity (PSES) affects over a third of individuals following stroke and negatively impacts on functional recovery, comfort and quality of life. Drug therapies have limited efficacy and unwanted side effects, botulinum toxin, although effective, is costly, and conventional electrical stimulation therapies are limited long term by habituation. We aim to investigate the efficacy of Sheffield Adaptive Patterned Electrical Stimulation (SHAPES), that delivers temporally and spatially varying pattern of electrical stimulation, against transcutaneous electrical stimulation (TENS) and standard care at reducing PSES.
Methods and design
Overall, 297 people with PSES will be randomised (1:1:1) to one of 3 arms: Standard care (no electrical stimulation), TENS (conventional patterned electrical stimulation) or SHAPES (adaptive patterned electrical stimulation). Both SHAPES and TENS are delivered using a specially designed electrical stimulation sleeve used for 60 minutes each day for 6-weeks. Outcome measures are completed at baseline, end of treatment (EOT 6 weeks) and then 6-weeks, 12-weeks and 24-weeks after the end of treatment. Efficacy will be determined based on the proportion of participants experiencing meaningful improvement (18%) in the 7-day Numerical Rating Scale (NRS-S) for PSES, compared between both intervention arms and standard care, and between the two intervention groups. Measures of arm motor function (Action Research Arm Test, MRC scale), and quality of life (SQoL-6D, EQ-5D) will also be measured along with a parallel health economic evaluation.
Discussion
The results of the SHAPES trial will inform management of elbow spasticity after stroke. The SHAPES intervention is a low cost, self-administered intervention for the management of spasticity that can be used repeatedly, and if found to be more effective than TENS or control has the potential to be widely implemented in the UK NHS healthcare setting. Furthermore, despite the wide use of TENS in the management of spasticity, this study will provide critically required evidence regarding its efficacy.
Development and validation of a prediction model for postoperative urinary retention after prolapse surgery: A retrospective cohort study
Authors: Kim Min Ju , Lee Sungyoung , Lee So Yeon , Oh Sumin , Jeon Myung Jae 
Published: March 2024

Abstract
Background
Postoperative urinary retention (POUR), a common condition after prolapse surgery with potential serious sequelae if left untreated, lacks a clearly established optimal timing for catheter removal. This study aimed to develop and validate a predictive model for postoperative urinary retention lasting > 2 and > 4 days after prolapse surgery.
Methods
We conducted a retrospective review of 1,122 patients undergoing prolapse surgery. The dataset was divided into training and testing cohorts. POUR was defined as the need for continuous intermittent catheterization resulting from a failed spontaneous voiding trial, with passing defined as two consecutive voids ≥ 150 mL and a postvoid residual urine volume ≤ 150 mL. We performed logistic regression and the predicted model was validated using both training and testing cohorts.
Results
Among patients, 31% and 12% experienced POUR lasting > 2 and > 4 days, respectively. Multivariable logistic model identified 6 predictors. For predicting POUR, internal validation using cross-validation approach showed good performance, with accuracy lasting > 2 (area under the curve [AUC] 0.73) and > 4 (AUC 0.75). Split validation using pre-separated dataset also showed good performance, with accuracy lasting > 2 (AUC 0.73) and > 4 days (AUC 0.74). Calibration curves demonstrated that the model accurately predicted POUR lasting > 2 and > 4 days (from 0 to 80%).
Conclusions
The proposed prediction model can assist clinicians in personalizing postoperative bladder care for patients undergoing prolapse surgery by providing accurate individual risk estimates.
Iatrogenic Dislocation of the Temporomandibular Joint due to General Anaesthesia
Authors: Alam Munir 
Published: March 2024

Abstract
Background: The temporomandibular joint (TMJ) is a modified hinge joint with the condyle of the mandible resting in a shallow glenoid fossa, predisposing it to be easily dislocated without extreme force. Anterior dislocation of TMJ is the most common type of dislocation. It can occur due to excessive opening of the mouth, trauma, or seizures. Iatrogenic cases of TMJ dislocation have been reported with 5% incidence during anaesthesia induction, intubation, and upper endoscopic procedures and surgical procedure performed on remote areas of the body (1,2). However, it has not been reported while the surgeon working around the head & neck area. In this case report while the surgeon operating on face, the dislocation of TMJ was missed or not identified in the immediate post operative period.
Case Presentation: This is a case report of 23 years old female in which post-intubation complication of TMJ dislocation occurred while the patient had facial surgery.
Conclusion: Early diagnosis of iatrogenic TMJ dislocation is required to prevent delays in treatment, whether the surgery in the head & neck region or remote area.