bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital reading comprehension instruction in English for children with English as an additional language: A systematic review
Authors: Murphy Annemarie , Arciuli Joanne 
Published: March 2024

BackgroundDigital literacy instruction is increasingly common in contemporary practices and can accommodate learners with a range of needs. This systematic review explores the use and effects of technology during reading comprehension instruction involving school‐aged children learning English as an additional language (EAL). Our aim was to provide broad, global coverage that reflects the state of research in the field.MethodsWe searched databases using terms related to EAL, reading comprehension and digital literacy instruction to identify relevant studies published on the topic between 2010 and 2023. Data were extracted regarding study context, participant characteristics, methodology, instruction characteristics and outcomes. We quantified the outcomes of instruction using standardised measures of effect size where possible and appraised the research quality of each study.ResultsA total of 4382 studies were identified. Of these, 53 met our inclusion criteria. Studies were conducted across a variety of low‐ and high‐resource environments, including countries where English is a majority language and those where it is a non‐majority language. Learners from kindergarten through 11th grade were included across the studies. A range of reading comprehension strategies were utilised within the context of digital instruction, with multiple‐strategy instruction the most common. Digital instruction resulted in statistically significant gains in reading comprehension in 32 studies (small to large effect sizes). Our research quality analysis revealed variability in terms of rigour, further adding to the diversity across studies. We conducted a sub‐analysis of eight studies that met the highest standards of research quality. Like the full set of 53 studies, these eight studies reported on diverse types of digital instruction and diverse reading comprehension outcomes but tended to result in smaller effect sizes.ConclusionsDigital reading comprehension instruction can result in reading comprehension gains for children learning EAL, though findings are mixed. Further high‐quality research is needed.
Multivariable prediction models for fetal macrosomia and large for gestational age: A systematic review
Authors: Ewington Lauren , Black Naomi , Leeson Charlotte , Al Wattar Bassel H. , Quenby Siobhan 
Published: March 2024

AbstractBackgroundThe identification of large for gestational age (LGA) and macrosomic fetuses is essential for counselling and managing these pregnancies.ObjectivesTo systematically review the literature for multivariable prediction models for LGA and macrosomia, assessing the performance, quality and applicability of the included model in clinical practice.Search strategyMEDLINE, EMBASE and Cochrane Library were searched until June 2022.Selection criteriaWe included observational and experimental studies reporting the development and/or validation of any multivariable prediction model for fetal macrosomia and/or LGA. We excluded studies that used a single variable or did not evaluate model performance.Data collection and analysisData were extracted using the Checklist for critical appraisal and data extraction for systematic reviews of prediction modelling studies checklist. The model performance measures discrimination, calibration and validation were extracted. The quality and completion of reporting within each study was assessed by its adherence to the TRIPOD (Transparent Reporting of a multivariable prediction model for Individual Prognosis Or Diagnosis) checklist. The risk of bias and applicability were measured using PROBAST (Prediction model Risk Of Bias Assessment Tool).Main resultsA total of 8442 citations were identified, with 58 included in the analysis: 32/58 (55.2%) developed, 21/58 (36.2%) developed and internally validated and 2/58 (3.4%) developed and externally validated a model. Only three studies externally validated pre‐existing models. Macrosomia and LGA were differentially defined by many studies. In total, 111 multivariable prediction models were developed using 112 different variables. Model discrimination was wide ranging area under the receiver operating characteristics curve (AUROC 0.56–0.96) and few studies reported calibration (11/58, 19.0%). Only 5/58 (8.6%) studies had a low risk of bias.ConclusionsThere are currently no multivariable prediction models for macrosomia/LGA that are ready for clinical implementation.
Effect of menopausal hormonal therapy on cardiovascular risks in Korean postmenopausal women: A nationwide cohort study
Authors: Yuk Jin‐Sung , Kim Gwang Sil , Byun Young Sup , Yang Seung‐Woo , Kim Myoung‐Hwan , Yoon Sang‐Hee , Seo Yong‐Soo , Kim Byung Gyu 
Published: March 2024

AbstractObjectiveTo evaluate the association between menopausal hormonal therapy (MHT) and the risk of cardiovascular disease (CVD), according to various regimens, dosages, routes of administration and starting ages of MHT.DesignA population‐based cohort study using the Korean National Health Insurance Services database.SettingNationwide health insurance database.PopulationWomen who reported entering menopause at an age of ≥40 years with no history of CVD in the national health examination.MethodsThe study population comprised 1 120 705 subjects enrolled between 2002 and 2019, categorised according to MHT status (MHT group, n = 319 007; non‐MHT group, n = 801 698).Main outcome measuresIncidence of CVD (a composite of myocardial infarction and stroke).ResultsThe incidence of CVD was 59 266 (7.4%) in the non‐MHT group and 17 674 (5.5%) in the MHT group. After adjusting for confounding factors, an increased risk of CVD was observed with the administration of tibolone (hazard ratio, HR 1.143, 95% CI 1.117–1.170), oral estrogen (HR 1.246, 95% CI 1.198–1.295) or transdermal estrogen (HR 1.289, 95% CI 1.066–1.558), compared with the non‐MHT group; the risk was based on an increased risk of stroke. The risk trends were consistent regardless of the age of starting MHT or the physicians’ specialty. Among tibolone users, a longer period from entering menopause to taking tibolone and the use of any dosage (1.25 or 2.5 mg) were linked with a higher risk of CVD, compared with non‐MHT users.ConclusionsThis nationwide cohort study demonstrated an increased risk of CVD, driven mainly by an increased risk of stroke, among tibolone and oral or transdermal estrogen users, compared with that of non‐MHT users.
Wallace melon juice fermented with Lactobacillus alleviates dextran sulfate sodium‐induced ulcerative colitis in mice through modulating gut microbiota and the metabolism
Authors: Zhang Junwei , Zhong Yu , Wang Danfeng , Zhu Jiangxiong , Deng Yun , Li Yuncheng , Liu Cong , Wang Ji‐Li‐Te , Zhang Minyan 
Published: March 2024

AbstractFermented foods have shown promise in preventing or treating ulcerative colitis (UC) via regulating intestinal flora and correcting metabolic disorders. However, the prevention effect of fermented Wallace melon juice (FMJ) on UC is unclear. In this study, the effects of FMJ on dextran sodium sulfate (DSS)‐induced UC were investigated via 16S rRNA sequencing and non‐targeted metabolomics. The results showed that FMJ was effective in alleviating the symptoms of UC, reducing histological damage and oxidative stress, decreasing the levels of pro‐inflammatory cytokines. After FMJ treatment, the level of propionic acid, butyric acid, and valeric acid increased by 14.1%, 44.4%, and 52.4% compared to DSS‐induced UC mice. Meanwhile, the levels of harmful bacteria such as Oscillospira, Bacteroidetes, and Erysipelotrichaceae and Clostridium decreased, while the levels of beneficial bacteria such as Akkermansia, Lactobacillus, and Bifidobacterium increased. Fecal metabolomics analysis identified 31 differential metabolites, which could regulate metabolic disorders in UC mice by controlling the primary bile acid biosynthesis, purine metabolism, and pantothenate and CoA biosynthesis pathway. Additionally, the abundances of butyric acid, bile acids, and pantothenic acid were positively correlated with Allobaculum, Bifidobacterium, and other beneficial bacteria (R2 > 0.80, p < 0.01). The results indicated that FMJ played a role in regulating the structure of intestinal flora, which in turn helped in repairing metabolic disorders and alleviated colitis inflammation.
Bioengineered xylanase from Misiones Argentina rainforest: A bakery enhancement approach
Authors: Molina Melisa A. , Cazzaniga Amanda , Sgroppo Sonia C. , Milde Laura B. , Zapata Pedro D. , Fonseca Maria I. 
Published: March 2024

AbstractIn this study, we pursued the heterologous expression of the xylanase gene from Trichoderma atroviride, a native fungus in the province of Misiones, and used it to enhance the textural properties of baked goods through varying enzymatic concentrations. This marks the inaugural exploration into its functionality in the context of bread production. The recombinant xylanase exhibited improved activity, reaching 36,292 U L−1, achieved by supplementing the culture medium with dextrose. Following the optimization of recombinant xylanase concentration, promising results emerged, notably reducing hardness and chewiness parameters of bread significantly. Our findings underscore the potential of this native fungal enzyme for industrial processes, offering a sustainable and efficient means to enhance the quality of baked goods with broad implications for the food industry. No prior research has been documented on the heterologous expression of the xylanase gene derived from T. atroviride, from the Misiones rainforest, expressed in Kluyveromyces lactis.Practical ApplicationThis research, focusing on the isolation and cloning of xylanase enzyme from Trichoderma atroviride, a native fungus in the province of Misiones, offers a valuable tool for improving the texture of bakery products. By optimizing enzyme concentrations, our findings present a practical approach for the food industry, offering a viable solution to improve the overall quality and consumer satisfaction of bakery products.
Predictive modeling of Salmonella spp. growth behavior in cooked and raw chicken samples: Real‐time PCR quantification approach and model assessment in different handling scenarios
Authors: Noviyanti Fia , Mochida Mari , Kawasaki Susumu 
Published: March 2024

AbstractThe increasing prevalence of Salmonella contamination in poultry meat emphasizes the importance of suitable predictive microbiological models for estimating Salmonella growth behavior. This study was conducted to evaluate the potential of chicken juice as a model system to predict the behavior of Salmonella spp. in cooked and raw chicken products and to assess its ability to predict cross‐contamination scenarios. A cocktail of four Salmonella serovars was inoculated into chicken juice, sliced chicken, ground chicken, and chicken patties, with subsequent incubation at 10, 15, 20, and 25°C for 39 h. The number of Salmonella spp. in each sample was determined using real‐time polymerase chain reaction. Growth curves were fitted into the primary Baranyi and Roberts model to obtain growth parameters. Interactions between temperature and growth parameters were described using the secondary Ratkowsky's square root model. The predictive results generated by the chicken juice model were compared with those obtained from other chicken meat models. Furthermore, the parameters of the chicken juice model were used to predict Salmonella spp. numbers in six worst‐case cross‐contamination scenarios. Performance of the chicken juice model was evaluated using the acceptable prediction zone from −1.0 (fail‐safe) to 0.5 (fail‐dangerous) log. Chicken juice model accurately predicted all observed data points within the acceptable range, with the distribution of residuals being wider near the fail‐safe zone (75%) than near the fail‐dangerous zone (25%). This study offers valuable insights into a novel approach for modeling Salmonella growth in chicken meat products, with implications for food safety through the development of strategic interventions.Practical ApplicationThe findings of this study have important implications in the food industry, as chicken juice could be a useful tool for predicting Salmonella behavior in different chicken products and thus reducing the risk of foodborne illnesses through the development of strategic interventions. However, it is important to recognize that some modifications to the chicken juice model will be necessary to accurately mimic all real‐life conditions, as multiple factors particularly those related to food processing can vary between different products.
Are there any completely sterile organs or tissues in the human body? Is there any sacred place?
Authors: Michán‐Doña Alfredo , Vázquez‐Borrego Mari C. , Michán Carmen 
Published: March 2024

AbstractThe human microbiome comprises an ample set of organisms that inhabit and interact within the human body, contributing both positively and negatively to our health. In recent years, several research groups have described the presence of microorganisms in organs or tissues traditionally considered as ‘sterile’ under healthy and pathological conditions. In this sense, microorganisms have been detected in several types of cancer, including those in ‘sterile’ organs. But how can the presence of microorganisms be detected? In most studies, 16S and internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing has led to the identification of prokaryotes and fungi. However, a major limitation of this technique is that it cannot distinguish between living and dead organisms. RNA‐based methods have been proposed to overcome this limitation, as the shorter half‐life of the RNA would identify only the transcriptionally active microorganisms, although perhaps not all the viable ones. In this sense, metaproteomic techniques or the search for molecular metabolic signatures could be interesting alternatives for the identification of living microorganisms. In summary, new technological advances are challenging the notion of ‘sterile’ organs in our body. However, to date, evidence for a structured living microbiome in most of these organs is scarce or non‐existent. The implementation of new technological approaches will be necessary to fully understand the importance of the microbiome in these organs, which could pave the way for the development of a wide range of new therapeutic strategies.
Intraoperative clonidine in endometriosis and spine surgery: A protocol for two randomised, blinded, placebo‐controlled trials
Authors: Birkebæk Stine , Lundsgaard Louise Møller , Juul Niels , Seyer‐Hansen Mikkel , Rasmussen Mikkel Mylius , Uhrbrand Peter Gaarsdal , Nikolajsen Lone 
Published: March 2024

AbstractBackgroundA high proportion of patients who undergo surgery continue to suffer from moderate to severe pain in the early postoperative period despite advances in pain management strategies. Previous studies suggest that clonidine, an alpha2 adrenergic agonist, administered during the perioperative period could reduce acute postoperative pain intensity and opioid consumption. However, these studies have several limitations related to study design and sample size and hence, further studies are needed.AimTo investigate the effect of a single intravenous (IV) dose of intraoperative clonidine on postoperative opioid consumption, pain intensity, nausea, vomiting and sedation after endometriosis and spine surgery.MethodsTwo separate randomised, blinded, placebo‐controlled trials are planned. Patients scheduled for endometriosis (CLONIPAIN) will be randomised to receive either 150 μg intraoperative IV clonidine or placebo (isotonic saline). Patients undergoing spine surgery (CLONISPINE) will receive 3 μg/kg intraoperative IV clonidine or placebo. We aim to include 120 patients in each trial to achieve power of 90% at an alpha level of 0.05.OutcomesThe primary outcome is opioid consumption within the first three postoperative hours. Secondary outcomes include pain intensity at rest and during coughing, nausea, vomiting and sedation within the first two postoperative hours and opioid consumption within the first six postoperative hours. Time to discharge from the PACU will be registered.ConclusionThis study is expected to provide valuable information on the efficacy of intraoperative clonidine in acute postoperative pain management in patients undergoing endometriosis and spine surgery.
Editorial: Using machine learning to predict significant fibrosis in metabolic dysfunction‐associated steatotic liver disease—authors' reply
Authors: Verma Nipun , De Arka , Duseja Ajay 
Published: March 2024

LINKED CONTENTThis article is linked to Verma et al papers. To view these articles, visithttps://doi.org/10.1111/apt.17891andhttps://doi.org/10.1111/apt.17902
Identification of COQ2 as a regulator of proliferation and lipid peroxidation through genome‐scale CRISPR‐Cas9 screening in myeloma cells
Authors: Li Miao , Zhang Chang‐lin , Zhou Di‐sheng , Chan Sze‐Hoi , Liu Xue‐Qi , Chen Shu‐Na , Yang Zi‐Yi , Ju Fei‐er , Sang Xiao‐yan , Liu Zi‐xuan , Zhang Qiao‐Xia , Pan Yu‐ming , Deng Si‐si , Wang Xiao‐Mei , Zhong Li , Zhang Xing‐Ding , Du Xin 
Published: March 2024

SummaryMultiple myeloma (MM) is the second most common malignant haematological disease with a poor prognosis. The limit therapeutic progress has been made in MM patients with cancer relapse, necessitating deeper research into the molecular mechanisms underlying its occurrence and development. A genome‐wide CRISPR‐Cas9 loss‐of‐function screening was utilized to identify potential therapeutic targets in our research. We revealed that COQ2 plays a crucial role in regulating MM cell proliferation and lipid peroxidation (LPO). Knockout of COQ2 inhibited cell proliferation, induced cell cycle arrest and reduced tumour growth in vivo. Mechanistically, COQ2 promoted the activation of the MEK/ERK cascade, which in turn stabilized and activated MYC protein. Moreover, we found that COQ2‐deficient MM cells increased sensitivity to the LPO activator, RSL3. Using an inhibitor targeting COQ2 by 4‐CBA enhanced the sensitivity to RSL3 in primary CD138+ myeloma cells and in a xenograft mouse model. Nevertheless, co‐treatment of 4‐CBA and RSL3 induced cell death in bortezomib‐resistant MM cells. Together, our findings suggest that COQ2 promotes cell proliferation and tumour growth through the activation of the MEK/ERK/MYC axis and targeting COQ2 could enhance the sensitivity to ferroptosis in MM cells, which may be a promising therapeutic strategy for the treatment of MM patients.
Associations between empirically proportionate and disproportionate fears of cancer recurrence and anxiety and depression in uveal melanoma survivors: Five‐year prospective study
Authors: Brown Stephen L. , Hope‐Stone Laura , van der Voort Nicola , Hussain Rumana , Heimann Heinrich , Coventry William L. , Cherry Mary Gemma 
Published: March 2024

AbstractObjectiveFear of cancer recurrence (FCR) may develop into elevated anxiety or depression symptoms, but few risk factors for this development are known. Objective recurrence risk estimation is possible in some cancers. Using theories of risk communication and phobias, we examined whether the proportionality of FCR to known objective recurrence risk influences the development of anxiety and depression symptoms.MethodUveal melanoma (UM) patients can opt for reliable prognostic testing. Patients experience either a ‘good’ or ‘poor’ prognostic outcome, whereby 10‐year mortality due to metastatic disease is, respectively, low or high. In a five‐year prospective study of a consecutive sample of 589 UM survivors, we used random intercept cross lagged panel analyses to examine whether proportionality differentially influences whether FCR progresses to anxiety and depression.ResultsPositive cross paths predicting anxiety from FCR were stronger in the poor prognosis group than the good prognosis and not tested groups. Prognostic group differences were not evident for depression.ConclusionsFCR was more likely to progress to elevated anxiety symptoms when proportionate to the known objective recurrence risk. Objective evidence may play a prominent role in the development and structure of fear because it assumes a high epistemic weight that activates a wide range of emotional and cognitive responses. Interventions that assist survivors to tolerate FCR in the presence of higher recurrence risks may be important in reducing anxiety symptoms.
Children's participation in everyday life: An international overview
Authors: Kosher Hanita , Gross‐Manos Daphna 
Published: March 2024

AbstractMuch of the research on children's right to participation has focused on formal settings, with less attention given to everyday contexts. The current study explores the scope of children's participation in everyday life in the family, school and community. Based on a sample of 46 000 children from 32 countries, children's participation was found to vary across the different contexts. Moreover, the quality of the children's relationships with adults was found to be positively related to the degree of their participation. Finally, some cross‐national differences were found.
Prevalence and factors associated with polydrug use among clients seeking treatment for alcohol misuse
Authors: Lawson Samuel , Bryant Jamie , Freund Megan , Dizon Joshua , Haber Paul S. , Shakeshaft Anthony , Jefferies Meryem , Farrell Michael 
Published: March 2024

AbstractIntroductionThe aim of this paper was to examine the client and psychosocial characteristics associated with polydrug use in patients with alcohol misuse as their primary drug of concern (PDC) seeking treatment from substance use treatment centres.MethodsSelf‐report surveys were undertaken with clients attending 1 of 34 community‐based substance use treatment centres across Australia with alcohol as their PDC. Survey items included client's socio‐demographic characteristics, level of alcohol dependence, use of other drugs including tobacco, health and wellbeing factors including health‐related quality of life. The factors associated with polydrug use (alcohol use concurrent with at least one other drug) were examined.ResultsIn a sample of 1130 clients seeking treatment primarily for alcohol problems, 71% reported also using another drug. The most frequently used drug was tobacco (50%) followed by cannabis (21%) and benzodiazepines (15%). Excluding tobacco use, 35% of participants reported polydrug use. Factors associated with any polydrug use were younger age, lower education levels, lower levels of mental health related quality of life and housing risk (i.e., risk of eviction or experienced homelessness in past 4 weeks). When tobacco was excluded, factors associated with polydrug use were age, lower physical and mental health‐related quality of life, and housing risk.Discussion and ConclusionsMost adults seeking treatment for alcohol misuse as their PDC reported using another drug in addition to alcohol. Treatment services should be designed accordingly to maximise the likelihood of treatment engagement and success.