bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein phosphatase 1α activity prevents oncogenic transformation
Authors: Liu Cathy W.Y. , Wang Rui‐Hong , Berndt Norbert 
Published: March 2006

AbstractCyclin‐dependent kinase 2 (Cdk2) phosphorylates Thr320 of protein phosphatase 1α (PP1α) in late G1, thereby inhibiting its activity. Phosphorylation‐resistant PP1αT320A, acting as a constitutively active (CA) mutant, causes a late G1 arrest by preventing the phosphorylation and inactivation of the retinoblastoma protein (pRb). Both PP1α‐mediated G1 arrest and PP1α phosphorylation in late G1 require the presence of pRb, indicating that PP1α is a crucial regulator of the pRb pathway, which is almost invariably mutated in human cancer. These findings prompted us to investigate whether PP1α interferes with oncogenic transformation. The ability of NIH 3T3 cells to form foci after transformation with ras/cyclin D1 was significantly inhibited by co‐transfection with PP1αT320A, but not PP1α. Likewise, cells expressing PP1αT320A or PP1αT320A fused to green fluorescent protein (GFP) were unable to form colonies in soft agar, regardless of whether PP1α constructs were co‐transfected with ras/cyclin D1 or transfected into stably transformed cells. Overexpressed wild‐type (Wt) PP1α and GFP‐PP1α were phosphorylated in Thr320, most likely explaining its lack of effect. Expression of GFP‐PP1αT320A was associated with caspase‐cleaved pRb in Western blots (WB) and morphological signs of cell death. These findings demonstrate that PP1α activity can override oncogenic signaling by causing cell‐cycle arrest and/or apoptosis rather than restoring contact inhibition or anchorage dependence. © 2006 Wiley‐Liss, Inc.
Creutzfeldt–Jakob disease presenting with alien limb sign
Authors: Fogel Brent , Wu Mark , Kremen Sarah , Murthy Kolar , Jackson George , Vanek Zeba 
Published: March 2006

AbstractCreutzfeldt–Jakob disease is a fatal spongiform encephalopathy, which typically presents with a rapidly progressing dementia and additional neurological findings that can be quite variable and diverse. Here we report the unusual case of a patient who presented with left alien limb sign without overt cognitive impairment and was ultimately diagnosed with pathologically confirmed Creutzfeldt–Jakob disease. © 2006 Movement Disorder Society
Effect of substrate temperature on the physical properties of dc magnetron sputtered Cu2O films
Authors: Sivasankar Reddy A. , Sreedhara Reddy P. , Uthanna S. , Venkata Rao G. , Klein A. 
Published: March 2006

AbstractCuprous oxide (Cu2O) films were formed by dc reactive magnetron sputtering onto glass substrates held at various temperatures in the range 303–648 K. The substrate temperature was found to be an important parameter in controlling the physical properties of the deposited films. The variation of cathode potential and deposition rate with the substrate temperature was studied. The dependence of crystallographic structure, electrical properties and optical absorption of the films on the substrate temperature was systematically investigated. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Restoration and colonization of freshwater mussels and fish in a southeastern United States tailwater
Authors: Layzer James B. , Scott Edwin M. 
Published: March 2006

AbstractThe French Broad River originates in North Carolina, flows west into Tennessee and at its confluence with the Holston River forms the Tennessee River. Douglas Dam, located on the French Broad River 52 km above its mouth, is operated primarily for peaking hydroelectric power and flood control. Prior to completion of the dam in 1943, the lower French Broad River contained about 53 species of freshwater mussels and 100 species of fish. By 1977, the fauna in the 52‐km‐long tailwater was reduced to 12 species of mussels and 42 native species of fish. Improvements in tailwater conditions occurred following initiation of minimum flows in 1987, and consistent reaeration of discharge in 1993. From 1988 to 2002, we sampled three sites (4, 28, and 39 km downstream of the dam) to monitor the fish assemblage. Each year since 1988, we have collected one or more additional species, indicating continued immigration. We collected 82 native and 9 exotic species of fish overall, but the maximum of 67 species in 1 year suggests that some species reside in the tailwater at low densities or all immigrants may not successfully colonize the tailwater. There is limited potential for most extirpated species of mussels to naturally recolonize the tailwater because source populations are isolated. Consequently, 19 754 adult mussels of 19 species were introduced between 1997 and 2000. Survival of translocated mussels has been high, and successful reproduction of at least one translocated species has occurred. Additionally, four mussel species are naturally colonizing the tailwater. Colonization and recruitment of additional mussel species is expected as populations of their host fishes increase. We believe that the improved conditions of the tailwater may allow for the re‐establishment of sustaining populations of 30 mussel species of historic occurrence, but the continued operation of Douglas Dam as a peaking hydroelectric project will reduce the probability of successfully reintroducing some species. Published in 2006 by John Wiley & Sons, Ltd.
Suitability of requirements prioritization methods for market‐driven software product development
Authors: Lehtola Laura , Kauppinen Marjo 
Published: March 2006

AbstractIn a company producing off‐the‐shelf software for mass markets, the future development steps of the products cannot be negotiated with one or few customers. The decisions concerning the priorities of the requirements must be made within the company, the developer bearing all the financial risks included. This means that finding the right priorities for the requirements is important. However, requirements prioritization is recognized as a difficult activity in software product development. The literature offers methods for requirements prioritization, but many authors report that practices in companies are mostly informal. In this study, we evaluated two requirements prioritization methods in industrial product development projects. In the first case, the users of the system evaluated thepair‐wise comparison techniquefor prioritizing user needs. In the second case, practitioners evaluatedWiegers' methodfor change requests. In addition, we interviewed 11 practitioners from 6 companies about their current requirements prioritization practices and the models that they use as a basis of their prioritization decisions. Our findings indicate that prioritization methods may have limited ability to support decision‐making in a complex area like requirements prioritization in market‐driven product development. In addition, there are practical difficulties in the usage of methods, and therefore, prioritization results should be taken more as being indicative than as an ultimate truth. Copyright © 2006 John Wiley & Sons, Ltd.
Global software development projects in one of the biggest companies in Latvia: is geographical distribution a problem?
Authors: Šmite Darja 
Published: March 2006

AbstractGlobal software development appears to be the latest trend in software engineering, bringing new opportunities as reaching mobility in resources, speeding time‐to‐market, obtaining extra knowledge, and increasing operational efficiency. Despite the popularity of the topic, there is a lack of research answering all the questions about how to perform in a distributed environment. This article describes research that aims to develop a framework for global project management and performance. In particular, the research shares information about the variety of collaboration models and project characteristics, and highlights areas of concern and specific risks examined in an observation of 19 distributed projects in a software development company in Latvia. The case study gives an overview of distributed projects emphasizing the specific risks such as organizational and cultural differences, language and time zone differences, lack of personal contact, and troubled communication over geographical distances. The preliminary research concludes that there is a necessity of specific methods and practices for effective project performance in a distributed environment. Copyright © 2006 John Wiley & Sons, Ltd.
A study on optimal scheduling for software projects
Authors: Padberg Frank 
Published: March 2006

AbstractSoftware projects often suffer from unexpected rework and delays. Therefore, project scheduling remains a difficult task for the managers. In this article, we compute optimal scheduling strategies for a set of sample software projects and simulate their behavior. The computations are based on a stochastic Markov decision model for software projects, which focuses on capturing the feedback between concurrent development activities. Since the underlying process model is stochastic, the strategies are stochastically optimal, that is, they minimize the expected project duration. The ultimate goal of this research is to develop guidelines for managers to schedule their software projects under uncertainty in the best possible way.The sample projects are similar, but differ in certain characteristics of the project or product, such as the strength of the coupling between the components or the degree of specialization of the teams on the tasks. By using a set of related projects, we can study how the project characteristics influence the optimal scheduling decisions in a project. After computing the optimal scheduling policies for the sample projects, we use extensive discrete‐event simulations to study the behavior of the optimal policy for each given setting and compare the performance of the optimal policy against the possible list policies. List policies are a simple and commonly used class of scheduling policies. For our sample projects, the simulations show that the best list policy in general is not optimal. The higher the degree of specialization of the teams, the larger the performance gap is. On the other hand, the stronger the coupling between the components, the smaller is the improvement that the optimal policy achieves over the best list policy. Copyright © 2006 John Wiley & Sons, Ltd.
The business of engineering
Authors: Strand Donald R. 
Published: March 2006

AbstractThe members comprising the Los Angeles Tall Buildings Structural Design Council are very active in activities relating to the design of tall buildings. The firms evolved from designing buildings of more moderate height during the 1960s to modern, tall, high‐rise buildings, which are now more easily designed with advanced computer systems and procedures. In these present procedures there is now more ‘paper’ developed in analysis and in construction administration, and requires a more hands‐on approach in a project. The authors in this special issue therefore are presenting information as to how they started, how they handle day‐to‐day projects and responsibilities, and where they think structural engineering design is headed. Copyright © 2005 John Wiley & Sons, Ltd.
1,25‐Dihydroxyvitamin D3 inhibits tumor necrosis factor‐α‐induced adhesion molecule expression in endothelial cells
Authors: Martinesi Maria , Bruni Sara , Stio Maria , Treves Cristina 
Published: March 2006

AbstractThis study tested the hypothesis that 1,25‐dihydroxyvitamin D3 [1,25(OH)2D3] plays a role in human umbilical vein endothelial cells (HUVEC) cultures. HUVEC were incubated with 10 or 100 nM 1,25(OH)2D3 for 24 h, in the absence or presence of 40 ng/ml tumor necrosis factor‐α (TNF‐α) or 2 ng/ml interleukin‐1α (IL‐1α). 1,25(OH)2D3 did not affect HUVEC viability and proliferation, while TNF‐α, alone or in combination with the hormone, significantly inhibited HUVEC viability. [3H]thymidine incorporation in HUVEC treated with TNF‐α or IL‐1α significantly decreased, in the absence or in the presence of the hormone, while the levels of vitamin D receptor markedly increased in the presence of 1,25(OH)2D3 alone or associated with TNF‐α or IL‐1α, in comparison to the control. The noteworthy increase in protein levels of intercellular adhesion molecule‐1 (ICAM‐1) and vascular cell adhesion molecule‐1 (VCAM‐1) induced by TNF‐α was significantly decreased after incubation of the cells with 1,25(OH)2D3, this effect not being seen on E‐selectin expression. Neither apoptosis nor nuclear translocation of NF‐κB, induced in HUVEC by TNF‐α was influenced by 1,25(OH)2D3 treatment.
An evaluation of the measurement of pain catastrophizing by the coping strategies questionnaire
Authors: Hirsh Adam T. , George Steven Z. , Riley Joseph L. , Robinson Michael E. 
Published: March 2006

AbstractMeasurement and conceptual issues of pain catastrophizing have been raised in the literature. The issues of construct redundancy and measurement overlap have received particular attention, with suggestions that measures of pain catastrophizing are confounded with measures of negative mood, namely depression. The current study sought to investigate these issues in the coping strategies questionnaire‐catastrophizing subscale (CSQ‐CAT), a widely used measure of pain catastrophizing. Chronic pain patients (n = 152) were recruited from the University of Florida pain clinics and completed a battery of psychological measures. Regression analyses indicated that measures of depression, anxiety, and anger accounted for 69% and 19% of the variance in measures of pain catastrophizing and pain, respectively. Trait anger and the cognitive and fearful dimensions of depression and anxiety were uniquely associated with pain catastrophizing. After controlling for measures of negative mood, pain catastrophizing contributed minimally to the prediction of pain. This study suggests that the CSQ‐CAT is highly related to measures of negative mood and raises doubts about its measurement of the construct of pain catastrophizing. Results also provide support for theoretical accounts of the relationships between pain catastrophizing, negative mood, and pain. Clinical implications, future research directions, and alternative measures of pain catastrophizing are discussed.
Increased seroprevalence of parvovirus B 19 IgG in complex regional pain syndrome is not associated with antiendothelial autoimmunity
Authors: Gross Oliver , Tschernatsch Marlene , Bräu Michael E. , Hempelmann Gunter , Birklein Frank , Kaps Manfred , Madlener Katharina , Blaes Franz 
Published: March 2006

AbstractThe etiology of complex regional pain syndrome (CRPS) is unclear yet. Recently autoantibodies and antecedent viral infections have been discussed to be involved in the pathogenesis of CRPS. We investigated sera from 39 CRPS patients and healthy controls for parvovirus B19 IgG and the occurrence of antiendothelial autoantibodies (AECA). CRPS patients showed a higher seroprevalence of parvovirus B19 IgG than controls (p<0.01). All CRPS 2 patients were positive. 10.2% of the CRPS patients and 10.0% of the controls had AECA (n.s.) and AECA were not associated with parvovirus B19 seropositivity. Our findings suggest the involvement of parvovirus B19, but not autoantibody‐mediated endothelial cell damage, in the pathogenesis of CRPS.
The association between experimental and clinical pain measures among persons with fibromyalgia and chronic fatigue syndrome
Authors: Geisser Michael E. , Gracely Richard H. , Giesecke Thorsten , Petzke Frank W. , Williams David A. , Clauw Daniel J. 
Published: March 2006

AbstractEvoked or experimental pain is often used as a model for the study of clinical pain, yet there are little data regarding the relationship between the two. In addition, there are few data regarding the types of stimuli and stimulus intensities that are most closely related to clinical pain.In this study, 36 subjects with fibromyalgia (FM), chronic fatigue syndrome (CFS), or both syndromes were administered measures of clinical pain and underwent a dolorimetry evaluation. Subjects also underwent experimental pain testing utilizing heat and pressure stimulation. Stimulation levels evoking low, moderate and high sensory intensity, and comparable levels of unpleasantness, were determined for both types of stimuli using random staircase methods. Clinical pain was assessed using visual analogue ratings and the short form of the McGill Pain Questionnaire (MPQ).Ratings of heat pain sensation were not significantly associated with clinical pain ratings, with the exception of unpleasantness ratings at high stimulus intensities. Pain threshold and tolerance as assessed by dolorimetry were significantly associated with average measures of clinical pain. Both intensity and unpleasantness ratings of pressure delivered using random staircase methods were significantly associated with clinical pain at low, moderate and high levels, and the strength of the association was greater at increasingly noxious stimulus intensities.These findings suggest that random pressure stimulation as an experimental pain model in these populations more closely reflects the clinical pain for these conditions. These findings merit consideration when designing experimental studies of clinical pain associated with FM and CFS.
Role of spinal microglia in rat models of peripheral nerve injury and inflammation
Authors: Clark Anna K. , Gentry Clive , Bradbury Elizabeth J. , McMahon Stephen B. , Malcangio Marzia 
Published: March 2006

AbstractMounting evidence supports the hypothesis that spinal microglia modulate the development and maintenance of some chronic pain states. Here we examined the role of spinal microglia following both peripheral inflammatory insult and peripheral nerve injury. We observed significant ipsilateral dorsal horn microglia activation 2 weeks after injury and bilateral activation 50 days following nerve injury as well as 24 h following intraplantar zymosan but not intraplantar complete Freund's adjuvant (CFA). Ipsilateral but not contralateral microglia activation was associated with hind paw mechanical hyperalgesia. Spinal injection of the glial metabolic inactivator fluorocitrate attenuated ipsilateral hyperalgesia and bilateral spinal microglia activation after peripheral nerve injury. Intrathecal fluorocitrate reversed hyperalgesia after intraplantar zymosan and produced no reversal of CFA‐induced hyperalgesia. These data suggest a role for spinal glia in the persistence of mechanical hyperalgesia following peripheral nerve injury. However, activation of spinal microglia contralaterally did not correlate to nociception. Furthermore, it would appear that the time course of microglia activation and their contribution to inflammatory pain is dependent on the inflammatory stimulus administered.
Prevalence and co‐occurrence of self‐rated pain and perceived health in school‐children: Age and gender differences
Authors: Sundblad Gunilla M. Brun , Saartok Tönu , Engström Lars‐Magnus T. ,   In collaboration with University College of Physical Education and Sports, Stockholm, Sweden
Published: March 2006

AbstractIn this nationwide study, 1975 students from grades 3, 6, and 9 (ages 9, 12, and 15 at the onset of the year), were recruited from randomly selected schools, which represented different geographical areas throughout Sweden.The main aim of the study was to assess the prevalence of self‐reported pain (headache, abdominal, and musculoskeletal pain) and perceived health (problems sleeping and/or if they often felt tired, lonely and sad). A second aim was to study the co‐occurrence among different pain and health variables. The students, (n=1908 distributed by grade 3: 255 girls and 305 boys, grade 6: 347 girls and 352 boys, grade 9: 329 girls and 320 boys) answered retrospectively (three months) a specially designed questionnaire.Fifty percent (50%) of the students reported that they had experienced pain, either as headache, abdominal pain or musculoskeletal pain, within the recall period. Gender differences were especially noticeable for headaches, where twice as many girls (17%, n=159) than boys (8%, n=80) reported that they suffered such pain at least once a week or more often. Co‐occurrence among the variables was moderate (0.3–0.5).For the total of the seven variables, the perception of pain and health complaints decreased with age for boys from grades 3 to 9, while multiple complaints increased for girls.
Evaluation of a modified interview version and of a self-rating version of the Suicide Assessment Scale
Authors: Niméus A. , Hjalmarsson Ståhlfors F. , Sunnqvist C. , Stanley B. , Träskman-Bendz L. 
Published: March 2006

AbstractThe Suicide Assessment Scale (SUAS) was constructed to be sensitive to change of suicidality. It was recently found to be predictive of suicide in a group of suicide attempters. The aim of the present study was to evaluate the reliability and validity of a modified interview version of SUAS with defined scores and also a new self-rating version (SUAS-S). The subjects consisted of former inpatients, 42 persons who had been admitted because of a suicide attempt about 12 years ago and 22 control patients. The subjects were rated according to the SUAS, the SUAS-S, as well as the Montgomery Asberg Depression Rating Scale (MADRS). The interrater reliability was found to be high. The SUAS correlated significantly with the MADRS, but the concordance was not consistent, which indicates that the SUAS measures something different from depression. The SUAS-S correlated significantly with the interview-rated SUAS, thus exhibiting good concurrent validity. In summary, both the modified interview version of SUAS and the SUAS-S seem to be valid, reliable and easily used suicide assessment instruments.
Meningocele and ankyloblepharon following in utero exposure to olanzapine
Authors: Arora M. , Praharaj S.K. 
Published: March 2006

AbstractAlthough atypical antipsychotics are widely used during pregnancy, their safety is not well established. This case highlights the possible teratogenic effect of olanzapine, in which the baby was born with meningocele and ankyloblepharon. It is suggested that olanzapine may interfere with embryonic development at different stages of pregnancy.