bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inefficacy of extracorporeal photochemotherapy in the treatment of B‐Cell chronic lymphocytic leukemia: Preliminary results
Authors: Wieselthier Janet S. , Japowicz Mary C. , Rothstein Thomas L. , Yu Thomas L. , Anderson Tom , Koh Howard K. 
Published: February 2007

AbstractBecause extracorporeal photopheresis has been shown to be clinically effective in Sezary syndrome, a disease characterized by a circulating malignant clone, we initiated a pilot study of its use in B‐cell chronic lymphocytic leukemia (B‐CLL) to see if it could be similarly effective. We treated three patients with Rai stage III and IV B‐CLL with photopheresis (3 consecutive days a week every 3 weeks) and followed serial clinical and immunologic parameters. While we noted no major toxicity, there was neither clinical response to treatment nor marked improvement in white counts or mitogen assays. We conclude that photopheresis was not effective in inducing remission in this pilot study of advanced stage B‐CLL patients. © 1992 Wiley‐Liss, Inc.
Effectiveness of high‐dose intravenous immunoglobulin in a case of acquired von willebrand syndrome with chronic melena not responsive to desmopressin and factor VIII concentrate
Authors: Castaman Giancarlo , Tosetto Alberto , Rodeghiero Francesco 
Published: February 2007

AbstractA patient with benign monoclonal lgG lambda paraproteinemia, acquired von Willebrand syndrome (AVWS), and chronic melena successfully responding to high‐dose intravenous immunoglobulin (lvlg) is reported. Coagulation parameters at admission were APTT (ratio) 1.68; VIII:C 11 IU/dL; vWF:Ag 7 IU/dL: Ricof <3 IU/dl. RIPA was >1.8 mg/ml, and bleeding time (BT) was prolonged (18 min). No evidence for an in vitro inhibitor against the VIII/vWF complex was observed. VIII/vWF measurements showed a short‐lived increase after both DDAVP and Hemate P, and BT was transiently normalized. After intravenous lg (1 g/kg for 2 days), VIII/vWF measurements, hemostatic parameters and multimeric pattern were completely corrected (VIII/C 106 IU/dl, vWF:Ag 168 IU/dl, RiC of 147 IU/dl, APTT ratio 0.89, BT 5′), with a return to pre‐infusion values after 15 days. Hemoccult test became negative and packed red cell transfusions, of which 130 units were administered during the last year, were no longer required. After 18 months the patient is on maintenance treatment with repeated courses of Ig, at 3 to 4‐week intervals based on VIII/vWF and BT monitoring. © 1992 Wiley‐Liss, Inc.
Dyserythropoiesis in iron‐deficiency anemia: Ultrastructural reassessment
Authors: Rozman Maria , Masat Teresa , Feliu Evarist , Rozman Ciril 
Published: February 2007

AbstractIron deficiency is usually included among the causes of acquired dyserythropoiesis. This concept was derived mainly from light microscopic studies. To reassess such a notion at ultrastructural level, a transmission electron microscopic evaluation of bone marrow was performed in seven patients with iron‐deficiency anemia. In contrast to the widely accepted concept, derived from light microscopic studies, only a small proportion (2–4%, not different from controls) of erythroblasts displayed some of the features of nuclear dyserythropoiesis. On the contrary, when examining the cytoplasm, we found a significantly increased number of void ropheocytotic vesicles in the majority of late erythroblasts as compared to controls (P < 0.001). This feature may be considered as an ultrastructural marker of iron deficiency and is consistent with the present knowledge on transferrin‐mediated delivery of iron to the cell. © 1992 Wiley‐Liss, Inc.
Human Lyb‐2 homolog CD72 is a marker for progenitor B‐cell leukemias
Authors: Schwarting Roland , Castello Roberto , Moldenhauer Gerhard , Pezzutto Antonio , Dörken Bernd , Hoegen Llka Von , Parnes Jane R. , Ludwig Wolf‐Dieter 
Published: February 2007

AbstractS‐HCL 2 is the prototype antibody of the recently defined CD72 cluster (human Lyb‐2). Under nonreducing conditions, S‐HCL 2 monoclonal antibody (mAb) precipitates a glycoprotein of 80–86 kDa. Under reducing conditions, a dimer of 43 and 39 kDa, with core proteins of 40 and 36 kDa, is precipitated. CD72 expression in normal and malignant tissues is different from expression of all other previously described human B‐cell antigens. In peripheral blood and bone marrow, the antigen appears to be present on all B lymphocytes, with the exception of plasma cells. In tissue, immunohistochemical staining revealed positivity for all known B‐cell compartments; however, pulpa macrophages of the spleen and von Kupffer cells exhibited distinct positivity for CD72 also. Among 83 malignant non‐Hodgkin's lymphomas examined by immunohistochemistry (alkaline phosphatase anti‐alkaline phosphatase technique), all 54 B‐cell lymphomas, including precursor B‐cell lymphomas, Burkitt's lymphomas, germinal center lymphomas, chronic lymphocytic leukemias, and hairy cell leukemias, were CD72 positive, but no T‐cell lymphomas were. Flow cytometry study of more than 80 mainly acute leukemias (52 B‐cell leukemias) showed reactivity with S‐HCL 2 mAb over the full range of B‐cell differentiation. In particular, very early B cells in cytoplasmic Ig (clg)‐negative, CD19‐positive prepre‐B‐cell leukemias and hybrid leukemias (mixed myeloid and B‐cell type) were consistently positive for CD72 on the cell surface. Therefore, CD72 may become an important marker for progenitor B‐cell leukemias. © 1992 Wiley‐Liss, Inc.
Pseudoxanthoma elasticum‐like skin lesions and angioid streaks in β‐thalassemia
Authors: Aessopos Athanasios , Savvides Panayotis , Stamatelos George , Tassiopoulos Thomas , Kaklamanis Phaedon , Fessas Phaedon , Rombos Loannis , Karagiorga Markisia 
Published: February 2007

AbstractOne hundred patients with homozygous or doubly heterozygous β‐thalassemia (62 with the major form and 38 with β‐thalassemia intermedia) were examined for signs of Pseudoxanthoma elasticum (PXE). Diagnostic skin lesions were found in 16 patients with either form of the basic disease. Twenty percent of all patients had angioid streaks (AS); both PXE skin lesions and AS were found in 10% of the patients; in all, 26% had either one or both of these manifestations. A positive correlation was found between the presence of one or both types of lesion and age of the patients (P = 0.032); there were no differences as regards ferritin and hematocrit levels, number of transfused units, chelation therapy, and splenic status between patients with PXE/AS findings and those without. The pathogenesis of these connective tissue manifestations at such a high frequency in β‐thalassemia is not clear; the possibilities of it's being acquired or inherited are discussed, the former being considered to be the more economical interpretation. © 1992 Wiley‐Liss, Inc.
IgD myeloma presenting as a testicular tumor: Establishment and characterization of an IgD‐secreting myeloma cell line
Authors: Ishii Kazuyoshi , Yamato Kenji , Kubonishi Ichiro , Taguchi Hirokuni , Ohtsuki Yuji , Miyoshi Isao 
Published: February 2007

AbstractA 72‐year‐old man presented with a left testicular tumor and underwent orchiectomy. The tumor was massively infiltrated with myeloma cells bearing monoclonal cytoplasmic IgDλ. Three months after orchiectomy, he developed huge abdominal masses and subsequently ascites containing numerous myeloma cells. An IgD‐secreting myeloma cell line, designated delta‐47, was established from the ascites. This cell line expressed CD4 and CD38, but lacked Fc and complement receptors, surface immunoglobulin, CD19, HLA‐DR, and PCA‐1. CD30 was detected on the cultured cells but not on the ascites tumor cells. Delta‐47 cells secreted the same immunoglobulin (IgDλ) as was found in the patient's serum. The light chain had a molecular weight of 35 kD which was larger than that of the normal light chain. Chromosome analysis of delta‐47 revealed an aneuploid karyotype with complex abnormalities including 1q+, 2p+, and 14q+. To our knowledge, this is the only IgD‐secreting myeloma cell line and would provide a useful tool for the study of IgD production and IgD myeloma. © 1992 Wiley‐Liss, Inc.
Relationship of plasma alpha tocopherol to index of clinical severity in individuals with sickle cell anemia
Authors: Phillips George , Tangney Christine C. 
Published: February 2007

AbstractSeveral parameters of micronutrition related to antioxidant activity are reduced in individuals with sickle cell anemia (SCA). The data presented here suggest that vitamin E, specifically alpha tocopherol, has a significant correlation (r = –0.38,P< 0.04) with a retrospective survey of clinical events and a significant correlation with a prospective survey of clinical events (r = –0.42,P< 0.03). The survey of clinical events score takes into account important vaso‐occlusive manifestations in SCA. Differences in plasma vitamin E levels explained about 15% of the variability in the clinical manifestations of SCA in the population studied. Previously reported data from our lab suggests that the differences in alpha tocopherol levels are not related to dietary intake. Vitamin E may play an important role in modulating the ability of sickle hemoglobin containing erythrocytes to produce vaso‐occlusion. Alternatively, it may simply be a marker of disease activity. Studies are ongoing at our center to determine if the relationship of alpha tocopherol to clinical events is causal. © 1992 Wiley‐Liss, Inc.
Essential thrombocythemia in pregnancy: Platelet count and pregnancy outcome
Authors: Chow Eudora Y. , Haley Lawrence P. , Vickars Linda M. 
Published: February 2007

AbstractWe report our experience of 4 successful pregnancies (including a pair of twins) in 3 women who have essential thrombocythemia (ET). All of the patients had a significant fall in platelet count (>20% of non‐pregnant state) during the course of the pregnancy. A review of the literature and our own experience suggests that the degree of platelet count reduction may be correlated with pregnancy outcome. Close monitoring of the platelet count is useful in the management of these cases. © 1992 Wiley‐Liss, Inc.
Interleukin‐2 production by primary adult T cell leukemia tumor cells is macrophage dependent
Authors: Arima Naomichi , Daitoku Yasuhisa , Hidakia Shiroh , Matsushita Kakushi , Ohtsubo Hideo , Fukumori Junko , Tanaka Hiromitsu , Yamamoto Yusei , Fujimoto Kouji , Onoue Kaoru 
Published: February 2007

AbstractWe have investigated the cellular requirements for IL‐2 production by autocrine proliferating tumor cells from four patients with adult T cell leukemia (ATL). Cultures of these ATL cells both produced endogenous IL‐2 protein in the absence of added mitogen and proliferated at higher levels when exogenous recombinant IL‐2 was added. Depletion of macrophages in the tumor cell cultures resulted in a sharp decline in tumor cell IL‐2 production, while re‐addition of macrophages reconstituted this response. Macrophage‐derived factors including IL‐6 and IL‐1 also reconstituted IL‐2 production in these macrophage depleted cultures. These results raise the possibility that macrophages may play a central role in HTLV‐I mediated immortalization of T cells. © 1992 Wiley‐Liss, Inc.
Clinical course and molecular characterization of a compound heterozygote for sickle hemoglobin and hemoglobin kenya
Authors: Waye John S. , Chui David H. K. , Cai Shi‐Ping , Eng Barry , Francombe William H. 
Published: February 2007

AbstractWe describe a 25‐year‐old black woman who presented with a long history of anemia requiring transfusions during childhood and adolescence. Molecular analysis revealed her to be a compound heterozygote for the sickle mutation and the ∼22.7 kb deletion associated with hemoglobin Kenya. This patient's clinical course was more severe than previously reported for the Hb S/Hb Kenya genotype, a probable consequence of concomitant iron deficiency. © 1992 Wiley‐Liss, Inc.
Defining the architecture of the red blood cell membrane: Newer biophysical approaches
Authors: Shohet Stephen B. , Bicknese Stephen E. 
Published: February 2007

AbstractFor many years the red cell membrane has served as an extraordinarily valuable model for membrane structure and function. During the past 2 decades, the biochemical concept of the membrane skeleton was established, and, with the help of electron microscopy, a partial depiction of this structure evolved. Newer biophysical approaches designed to measure distances between various components of membrane skeleton as well as distances between the membrane skeleton and the overlying bilayer should now help to define this structure more realistically. Fluorescence resonance energy transfer, single photon radioluminescence, and total internal reflectance are three biophysical techniques that will enable us to measure such distances over a substantial range, which extends from a few Angstroms to ∼2 μm. The ability to make such measurements in intact cells and in fully hydrated, undenatured membrane preparations should add a new dimension to our understanding of the structure of the red cell membrane. © 1993 Wiley‐Liss, Inc.
Mechanism of red blood cell aging: Relationship of cell density and cell age
Authors: Piomelli Sergio , Seaman Carol 
Published: February 2007

AbstractThe human red cell has a life span of 120 days. The mechanism that determines cell removal from the circulation with such precision remains unknown. Most studies of red cell aging have been based on analysis of cells of progressively increasing age separated by density. The relationship between red cell age and density has been recently challenged, and the hypothesis has been put forward that cell death is not the result of a progressive deterioration of essential cell constituents. This theory was based on preliminary observations in transient erythroblastopenia of childhood, which could not later be confirmed. When the relationship between cell aging and increasing density is critically reviewed, it appears to be based on firm experimental evidence, confirmed by in vivo demonstration of decreasing survival of cells of increasing age. Analysis of studies using buoyant density gradients reveals that this technique can easily distinguish the single exponential slope of decline for those cell components that change progressively throughout the red cell life span from the biphasic decline of those that decrease drastically at the reticulocyte‐mature red cell transition. The view that the aging of the red cell and its removal from the circulation result from a progressive series of events during the 120 days of its life span appears to be the most consistent with the available data. Density separation, validated by much experimental evidence, remains a most useful technique for the study of the mechanism of aging of the red cell. © 1993 Wiley‐Liss, Inc.
Cobalamin absorption and hematologic status after two types of gastric surgery for obesity
Authors: Yale Charles E. , Gohdes Paul N. , Schilling Robert F. 
Published: February 2007

AbstractA series of morbidly obese patients was treated surgically with a gastric bypass, and a subsequent series received a vertical banded gastroplasty.To compare some of the nutritional effects of these two procedures we measured serum vitamin B12 levels, absorption of food vitamin B12, frequency of microcytosis of erythrocytes, and frequency of anemia at one or more years after surgery.Patients with a gastric bypass showed greater weight loss, a greater frequency of microcytosis and anemia, more frequent subnormal serum levels of vitamin B12, and impressive failure to absorb food vitamin B12. Boiling the food containing vitamin B12 led to increased absorption. © 1993 Wiley‐Liss, Inc.
Some properties of hemoglobin A2
Authors: Ranney Helen M. , Lam Ruby , Rosenberg Gwen 
Published: February 2007

AbstractWhile no significant physiologic function of hemoglobin A2 (Hb A2), the minor basic component of human hemoglobin, has been recognized, only its oxygen equilibria have been studied in detail. Since hemoglobin A2 and its oxidative denaturation product, hemichrome A2, bind to the red cell membrane, particularly to band 3, to a greater extent than do Hb A or hemichrome A, some of the properties of Hb A2 that might influence hemoglobin‐membrane association were examined. Hemoglobin A2 exhibited slightly increased susceptibility to autoxidation to methemoglobin. No differences were noted between methemoglobins A and A2, including the rates of enzymatic reduction and stability of the heme‐globin linkage. Oxyhemoglobin A2 had a slightly lower solubility in phosphate buffer than did hemoglobin A. While the hemichromes (prepared with phenylhydrazine) of hemoglobins A2 and A had the same optical spectra, the A2 hemichrome exhibited greater stability. It is suggested that the differences in products of oxidative denaturation may provide the basis for functional differences between hemoglobins A2 and A. © 1993 Wiley‐Liss, Inc.
Clinical and prognostic significance of in vivo differentiation in acute myeloid leukemia
Authors: Raza Azra , Preisler Harvey , Lampkin Beatrice , Lykins Joseph , Kukla Cathy , Gartside Peter , Sheikh Yasin , Yousuf Naveed , White Michael , Barcos Maurice , Bennett John , Browman George , Goldberg Jack , Grunwald Hans , Larson Richard , Vardiman James , Vogler Ralph 
Published: February 2007

AbstractBromodeoxyuridine (BrdU) was administered to 86 newly diagnosed patients with standard risk acute myeloid leukemia (AML) prior to starting induction therapy and the labeling index (LI), durations of S‐phase (Ts), and the cell cycle (Tc) of myeloblasts were determined. Induction therapy with cytosine arabinoside and daunomycin was subsequently started. Bone marrow biopsies were obtained on days 6 and 17 and weekly thereafter, and were treated with a monoclonal anti‐BrdU antibody to determine the fate of cells labeled on day 0 by BrdU. BrdU labeled granulocytes indicating the presence of in vivo differentiation (Diff+) were identified in 48 patients ranging from 1+ (1–10 labeled cells) to 4+ (greater than 31 labeled granulocytes). When compared to 38 differentiation negative (Diff‐) patients, Diff+ group had longer Ts (14.5 hr vs. 10.95 hr, P = 0.015) and Tc (59.7 hr vs. 41.7 hr, P = 0.017). Remission duration was significantly longer (no median) for 3–4+ Diff+ as compared to Diff‐ (median = 220 days) patients (Wilcoxon P = 0.04). We conclude that the detection of in vivo differentiation in AML patients indicates a favorable long‐term prognosis either due to the presence of a substantial amount of normal residual hematopoiesis prior to starting induction therapy or due to the ability of leukemic cells to undergo differentiation. © 1993 Wiley‐Liss, Inc.
New assay for the rapid determination of plasma holotranscobalamin II levels: Preliminary evaluation in cancer patients
Authors: Vu Thu , Amin Jyoti , Ramos Mercedita , Flener Victoria , Vanyo Lori , Tisman Glenn 
Published: February 2007

AbstractWe describe a new and rapid assay for the measurement of plasma B12 bound to transco‐balamin II (holotranscobalamin II) using the property of adsorption of the polypeptides of apotranscobalamin II and holotranscobalamin II to the hydrophobic surface of microfine glass particles. Acid‐washed microfine glass was used to separate vitamin B12 bound to the glycoproteins transcobalamin I and transcobalamin III (haptocorrin or R binder) from that bound to transcobalamin II. Sephadex gel filtration separation of 57Co‐labelled vitamin B12 binders confirmed that >90% of holotranscobalamin II can be removed from plasma holohaptocorrin by adsorption to microfine glass particles. Since only holotrans‐cobalamin II is capable of delivering vitamin B12 to metabolizing cells, plasma holotrans‐cobalamin II content reflects the availability of B12 to cells. Use of this test in cancer patients undergoing either chemotherapy or radiation therapy revealed evidence of early negative B12 balance that in some instances was induced by the treatment itself. © 1993 Wiley‐Liss, Inc.
Comparison of acute myeloid leukemia occurring de novo or preceded by a myelodysplastic stage: Differences in cellular DNA content
Authors: Widell S. , Auer G. , Hast R. , Reizenstein P. 
Published: February 2007

AbstractThe DNA content of bone marrow cells in patients with acute leukemia preceded by a myelodysplastic stage (MDS‐AML) was compared to that in patients with de novo AML. We studied granulocytes, lymphocytes, monocytes, and blasts/promyelocytes from Feul‐gen‐stained bone marrow smears of 11 patients with de novo AML, ten patients with MDS‐AML, and 13 apparently healthy controls. The mean amount of DNA per cell (DNA index; Dl) in each cell population was determined using a digital video‐based imageanalyzing system (CAS‐100). Analysis of variance (F test) showed a significant difference in the DNA content between de novo AML on one hand and MDS‐AML and controls on the other as regards to blasts/promyelocytes (P < 0.01), lymphocytes (P < 0.05), and monocytes (P < 0.01), respectively. In three of 11 (27%) patients with de novo AML, a lower than normal limit Dl was found both in immature and mature bone marrow cells. Patients with MDS‐AML had those of Dl values similar to normal controls. In consequence, a significantly reduced mean Dl was found in patients with de novo AML in blasts/promyelocytes (P < 0.01), and monocytes (P < 0.05) compared to both normal controls and MDS‐AML. Together with data published separately, suggesting differences in granulocyte morphology, clonality, and HLA‐DR expression, these data suggest biological differences between the two diseases. © 1993 Wiley‐Liss, Inc.
Successful bone marrow transplantation in children with severe aplastic anemia using HLA‐partially matched family donors
Authors: Locatelli Franco , Porta Fulvio , Zecca Marco , Pedrazzoli Paolo , Maccario Rita , Giani Simona , Vitale Vito , Martinetti Myriam , Mazzolari Evelina , Lanfranchi Arnalda , Nespoli Luigi , Bacigalupo Andrea , Severi Francesca 
Published: February 2007

AbstractBone marrow transplantation (BMT) using HLA‐partially matched family donors has produced disappointing results (25–30% of long‐term survivors) in patients with severe aplastic anemia. We describe two children affected by severe aplastic anemia, not responsive to immunosuppressive therapy, who underwent allogeneic bone marrow transplantation using a HLA‐partially matched family donor. Both cases presented 2 first class HLA‐antigens (A and B) disparity between donor and recipient. The pretransplant conditioning regimen consisted of cyclophosphamide, thoracoabdominal irradiation, cytosine‐arabinoside, and antilymphocyte globulin. As graft versus host disease (GVHD) prophylaxis, Cyclosporine‐A was administered at usual dosages for 6 months. A full marrow engraftment was observed in both cases. Only grade I acute GVHD, promptly responsive to corticosteroid therapy, developed with no chronic GVHD. Five months after transplant, both children progressively developed hypertension, renal function impairment, thrombocytopenia, and severe normochromic anemia, with erythropoietin serum levels lower than expected for the haematocrit. After antihypertension treatment and supportive therapy, the clinical picture progressively improved, while treatment with recombinant human erythropoietin completely corrected the long‐lasting anemia. The two children are alive and well 28 months after the transplant, with a Karnofsky score of 100% and a normal peripheral blood count. The authors suggest that, once immunosuppressive therapy has failed, BMT from donors other than HLA‐identical sibling is a feasible approach in children affected by severe aplastic anemia, not having an HLA‐identical donor. © 1993 Wiley‐Liss, Inc.