bluuebunny/crossref_metadata_2025_split huggingface dataset:
Knee osteoarthritis in former runners, soccer players, weight lifters, and shooters
Authors: Kujala Urho M. , Kettunen Jyrki , Paananen Heli , Aalto Teuvo , Battié Michele C. , Impivaara Olli , Videman Tapio , Sarna Seppo 
Published: February 2007

AbstractObjective. To determine the relationship between different physical loading conditions and findings of knee osteoarthritis (OA).Methods. We selected 117 male former top‐level athletes (age range 45–68 years) who had participated in sports activities with distinctly different loading conditions: 28 had been long‐distance runners, 31 soccer players, 29 weight lifters, and 29 shooters. Histories of lifetime occupational and athletic knee loading, knee injuries, and knee symptoms were obtained, and subjects were examined clinically and radiographically for knee findings of OA.Results. The prevalence of tibiofemoral or patellofemoral OA based on radiographic examination was 3% in shooters, 29% in soccer players, 31% in weight lifters, and 14% in runners (P = 0.016 between groups). Soccer players had the highest prevalence of tibiofemoral OA (26%), and weight lifters had the highest prevalence of patellofemoral OA (28%). Subjects with radiographically documented knee OA had more symptoms, clinical findings, and functional limitations than did subjects without knee OA. By stepwise logistic regression analysis, the risk for having knee OA was increased in subjects with previous knee injuries (odds ratio [OR] 4.73), high body mass index at the age of 20 (OR 1.76/unit of increasing body mass index), previous participation in heavy work (OR 1.08/work‐year), kneeling or squatting work (OR 1.10/work‐year), and in subjects participating in soccer (OR 5.21).Conclusion. Soccer players and weight lifters are at increased risk of developing premature knee OA. The increased risk is explained in part by knee injuries in soccer players and by high body mass in weight lifters.
Hla–dr8 and acute anterior uveitis in ankylosing spondylitis
Authors: Monowarul Islam S. M. , Numaga Jiro , Fujino Yujiro , Masuda Kanjiro , Ohda Hiromi , Hirata Ranko , Maeda Hiroo , Mitsui Hiroshi 
Published: February 2007

AbstractObjective. To identify possible factors in the development of acute anterior uveitis (AUU) in patients with ankylosing spondylitis (AS).Methods. We investigated HLA antigens serologically, and HLA–DRB1*08 alleles by polymerase chain reaction–restriction fragment length polymorphism, in 42 Japanese AS patients with and without AAU.Results. Thirty‐six of the AS patients (85.7%) had HLA–B27. Thirteen (65%) of the 20 patients with AAU had HLA–DR8, whereas only 1 (4.5%) of the 22 patients without AAU had DR8. The difference was statistically significant (Pcorr < 0.001).Conclusion. Our data suggest that HLA–B27 is strongly associated with AS in Japanese patients and that HLA–DR8 is important for the development of AAU in Japanese patients with AS.
The prevalence and incidence of systemic lupus erythematosus in Birmingham, England
Authors: Johnson Angela E. , Gordon Caroline , Palmer Robert G. , Bacon Paul A. 
Published: February 2007

AbstractObjective. To establish the point prevalence of systemic lupus erythematosus (SLE) on January 1, 1992, and the incidence of SLE during 1991, in Birmingham, England, a large city with a broad ethnic mix.Methods. Six sources were used to ascertain patients with diagnosed SLE, including notification by attending and primary care physicians, the lupus patient support group, and hospital inpatient and laboratory data.Results. There were 242 SLE patients (227 females, 15 males) identified: prevalence rate 27.7/100,000 (95% confidence interval 24.2–31.2/100,000) in the population and 206.0/100,000 in Afro‐Caribbean females. No significant differences in female ethnic prevalence rates by place of birth were observed. Thirty‐three patients developed SLE in 1991: incidence rate 3.8/ 100,000/year (95% confidence interval 2.5–5.1/100,000/year).Conclusion. This study illustrates dramatic differences in incidence and prevalence rates in the UK, depending on ethnic group and irrespective of place of birth.
The arthritis knowledge questionnaire
Authors: Edworthy Steven M. , Devins Gerald M. , Watson Margaret M. 
Published: February 2007

AbstractObjective. To develop a questionnaire for measuring patients' knowledge about arthritis and its self‐management.Methods. An initial item pool of 175 multiple‐choice questions was developed, based on the Stanford Arthritis Self‐Management Program and on basic medical knowledge about arthritis and its management. A rheumatologist, psychologist, physiotherapist, and several lay individuals assessed the questions for content validity, breadth of coverage, and face validity. Patients assessed the questions for comprehensibility. Four subject groups with varying knowledge levels were recruited to answer the entire set of questions. Item analysis was undertaken to develop parallel forms of subtests so that repeated assessments could be undertaken.Results. The final instrument, the Arthritis Knowledge Questionnaire, consists of 2 tests, each comprising 3 subtests: self‐management (15 items), arthritis in general (15 items), and rheumatoid arthritis (11 items).Conclusion. We present psychometric evidence that these parallel forms provide highly reliable and valid estimates of knowledge concerning arthritis and arthritis self‐management.
An analysis of forty‐two wegener's granulomatosis patients treated with methotrexate and prednisone
Authors: Sneller Michael C. , Hoffman Gary S. , Talar‐Williams Cheryl , Kerr Gail S. , Hallahan Claire W. , Fauci Anthony S. 
Published: February 2007

AbstractObjective. To determine the efficacy of low‐dose methotrexate (MTX) plus prednisone in the treatment of Wegener's granulomatosis (WG).Methods. An open‐label study of weekly low‐dose MTX plus prednisone for the treatment of WG was performed. Forty‐two patients who did not have immediately life‐threatening disease were enrolled into the study. Outcome was determined by clinical characteristics and pathologic, laboratory, and radiographic findings.Results. Weekly administration of MTX and prednisone resulted in remission of disease in 30 of the 42 patients (71%). The median time to remission was 4.2 months. The estimated median time to relapse for all patients in whom remission was achieved was 29 months. Eight patients who had relapses were treated with a second course of MTX plus prednisone, and a second remission was induced in 6 of the 8 (75%).Conclusion. Weekly low‐dose MTX was shown in this study to be an acceptable alternative form of therapy for selected patients with WG who do not have immediately life‐threatening disease or who have developed serious cyclophosphamide‐associated toxicity.
Complications resulting from the use of chinese herbal medications containing undeclared prescription drugs
Authors: Gertner Elie , Marshall Peter S. , Filandrinos Dean , Potek Arnold S. , Smith Theresa M. 
Published: February 2007

AbstractObjective. Many patients with chronic disease use alternative therapies. Our objective was to investigate complications resulting from the use of Chinese herbal medications containing undeclared prescription drugs, and to analyze these pills.Methods. Medical records of 5 patients with complications were reviewed. Pills from symptomatic and asymptomatic individuals were analyzed for possible content of undeclared prescription drugs.Results. All pills analyzed contained mefenamic acid and diazepam. Complications related to the presence of these substances included, among others, massive gastrointestinal bleeding.Conclusion. Chinese herbal medications may contain undeclared prescription drugs including nonsteroidal antiinflammatory drugs and benzodiazepines.
Antineutrophil cytoplasmic antibodies in systemic lupus erythematosus
Authors: Schnabel Armin , Csernok Elena , Isenberg David A. , Mrowka Christian , Gross Wolfgang L. 
Published: February 2007

AbstractObjective. To examine the prevalence, subspecificities, and clinical associations of antineutrophil cytoplasmic antibodies (ANCA) in patients with systemic lupus erythematosus (SLE).Methods. One hundred fifty‐seven sera from 120 patients with SLE were examined for classic (c) and perinuclear (p) pattern ANCA by indirect immunofluorescence. Antibody subspecificities were determined by enzyme‐linked immunosorbent assay (ELISA). Serologic results were correlated with clinical manifestations as categorized by the BILAG (British Isles Lupus Assessment Group) index.Results. ANCA were found in 40 of the 157 sera (25%). Only a pANCA, not a cANCA, pattern of fluorescence was seen. By ELISA testing, 16 sera reacted to lactoferrin, 8 to elastase, and 4 to lysozyme. There was no reactivity to proteinase 3 (PR3) or myeloperoxidase (MPO). No correlation of pANCA, or any of the ANCA subspecificities, with organ system involvement, as categorized by the BILAG index, was found. Notably, there was no correlation of ANCA results with lupus vasculitis.Conclusion. The absence of cANCA, anti‐PR3, and anti‐MPO shows that with appropriate assay conditions, ANCA testing assists in the differentiation between SLE and the ANCA‐associated vasculitides. The lack of a correlation between pANCA or any ANCA subspecificity and clinical manifestations suggests that ANCA do not identify particular clinical subsets among SLE patients, including those with lupus vasculitis.
Absence of parvovirus b19 infection in chronic fatigue syndrome
Authors: Ilaria Robert L. , Komaroff Anthony L. , Fagioli Laura R. , Moloney William C. , True Cheryl A. , Naides Stanley J. 
Published: February 2007

AbstractObjective. To evaluate the presence of infection with parvovirus B19 in patients with chronic fatigue syndrome (CFS) who also had rheumatologic symptoms and mild hematologic abnormalities.Methods. Seven patients meeting the Centers for Disease Control and Prevention working case definition for CFS who also had mild leukopenia, thrombocytopenia, or anemia were studied. Bone marrow was aspirated from each patient, and examined for morphologic abnormalities, including features seen in marrow infections with parvovirus B19, as well as for parvoviral DNA, using polymerase chain reaction (PCR) amplification. Serum obtained at the time of marrow aspiration was also evaluated for parvoviral DNA, using the PCR method, and was examined for the presence of IgM and IgG antibodies to the virus.Results. No evidence of marrow involvement with parvovirus B19 was found in any patient. One patient had antibody evidence of a transient parvoviral infection, during which time an underlying thrombocytopenia worsened.Conclusion. Despite examining a selected group of patients thought most likely to have parvoviral infection, based on clinical and hematologic measures, no evidence of clinically important parvoviral infection was noted. Thus, it seems unlikely that parvovirus B19 plays a role in CFS, even though it has been associated with fibromyalgia, a clinically similar syndrome.
Dysregulation of the in vivo production of interleukin‐1 receptor antagonist in patients with rheumatoid arthritis pathogenetic implications
Authors: Chikanza Ian C. , Roux‐Lombard Pascal , Dayer Jean‐Michel , Panayi Gabriel S. 
Published: February 2007

AbstractObjective. Patients with rheumatoid arthritis (RA) have defective hypothalamic responses to inflammation, possibly because of excessive production of cytokine inhibitor, which could blunt the effects of cytokines on the hypothalamus, or because of an imbalance between interleukin‐1β (IL‐1β) and interleukin‐1 receptor antagonist (IL‐1Ra), which could create a mainly proinflammatory state. The present study was undertaken to investigate these possibilities.Methods. The in vivo kinetics of IL‐1β and IL‐1Ra secretion were studied in patients with RA, osteoarthritis (OA), and chronic osteomyelitis (OM), and in normal controls before and after surgery.Results. The 24‐hour levels of IL‐1Ra were significantly increased in RA (P < 0.001), but there was no diurnal variation in any group. Preoperative levels of IL‐1Ra were higher in RA and OA sera (P = 0.001). After surgery, IL‐1Ra behaved like an acute‐phase reactant protein in all subjects. IL‐1β was 10–20 times higher in RA than in OM and OA patients at baseline, but the percentage increase in all groups postoperatively was the same. RA patients had an IL‐1Ra:IL‐1β ratio of 26.2 ± 3.7 (mean ± SEM) at baseline (OM patients 89.2 ± 5.8 and OA patients 1,310 ± 363); this increased to 66.5 ± 19.8 after surgery (OM patients 120 ± 6.7 and OA patients 325.8 ± 106).Conclusion. RA patients have a dysregulation of IL‐1Ra production, and it seems unlikely that the defective hypothalamic response seen in RA is due to a functional deficit of IL‐1β.
Cd28 expression on t cell subsets in vivo and cd28‐mediated t cell response in vitro in patients with rheumatoid arthritis
Authors: Sfikakis Peter P. , Zografou Amalia , Viglis Vassilis , Iniotaki‐Theodoraki Aliki , Piskontaki Ifigenia , Tsokos George C. , Sfikakis Paul , Choremi‐Papadopoulou Helen 
Published: February 2007

AbstractObjective. In view of the critical importance of the CD28–CD80 (B7/BB1) costimulatory pathway in antigen‐specific T cell activation and clonal expansion, we examined CD28 surface molecule expression in vivo, and T cell receptor/CD3‐mediated and B7/BB1‐costimulated T cell proliferation in vitro, in rheumatoid arthritis (RA).Methods. Two‐color immunofluorescence analyses of peripheral blood and synovial fluid–derived T cells, as well as 3H‐thymidine incorporation assays, were performed.Results. In the peripheral blood of 31 patients with active, untreated RA, a mean of 91% (range 48–100%) of CD4+ and 46% (range 13–82%) of CD8+ T cell subsets were CD28+, which was not significantly lower than normal. Although an overall decrease in the number of T cells was not observed, the numbers of CD28+CD8+ T cells were significantly lower in RA patients (mean 233/μl, versus 292/μl in controls), and this decrease was more pronounced in patients with severe disease (mean 172/μl). CD28 expression on peripheral CD8+ T cells in RA patients, but not in healthy individuals, correlated inversely with T cell activation as assessed by HLA–DR antigen expression. In contrast to the peripheral blood, RA synovial fluid T cells were almost exclusively CD28+, suggesting that migration of CD28+CD8+ T cells to active sites of inflammation may occur. In vitro proliferative responses of peripheral blood T cells to B7/BB1 costimulation in the presence of mitogenic doses of anti‐CD3 monoclonal antibody were identical in patients with RA and healthy individuals.Conclusion. Functionally intact CD28+ T cells may contribute to the abnormal immunoregulation and joint inflammation in RA.
Transforming growth factor β1, a major stimulator of hyaluronan synthesis in human synovial lining cells
Authors: Haubeck Hans‐Dieter , Kock RÜDiger , Fischer Dagmar‐Christiane , Leur Eddy Van De , Hoffmeister Karin , Greiling Helmut 
Published: February 2007

AbstractObjective: To investigate the role of cytokines and growth factors in the regulation of hyaluronan synthesis in human synovial lining cells.Methods. Synovial lining cells were obtained from human knee joints, isolated by the explant method, and characterized by immunocytochemistry using monoclonal antibodies against monocyte/macrophage markers as well as antibodies against hyaluronan synthase. After stimulation by cytokines and growth factors, hyaluronan was measured by radiometric assay. The molecular weight distribution of the hyaluronan synthesized was determined by high‐performance gelpermeation liquid chromatography. To test the effect of oxygen‐derived free radicals, the concentration and molecular weight distribution of hyaluronan were determined in the presence and absence of catalase and superoxide dismutase.Results. Hyaluronan synthesis was stimulated in synovial lining cells by transforming growth factor β1 (TGFβ1), interleukin‐1β (IL‐1β), and to a lesser extent by tumor necrosis factor α (TNFα). Analysis of the molecular weight distribution of hyaluronan after stimulation of synovial lining cells with TGFβ1, IL‐1β, and TNFα indicated that hyaluronan is synthesized in a high molecular weight form and might be degraded in the course of inflammatory processes by oxygen‐derived free radicals.Conclusion. Our findings suggest that TGFβ1 is a major stimulator of hyaluronan synthesis in human synovial lining cells and might be involved in the pathogenic mechanisms of joint swelling in inflammatory and degenerative joint diseases.
Eda‐containing fibronectin is synthesized from rheumatoid synovial fibroblast‐like cells
Authors: Hino Kazuo , Shiozawa Shunichi , Kuroki Yasuo , Ishikawa Hitoshi , Shiozawa Kazuko , Sekiguchi Kiyotoshi , Hirano Hisanobu , Sakashita Eiji , Miyashita Keiichi , Chihara Kazuo 
Published: February 2007

AbstractObjective. To identify the cells that synthesize EDA‐containing fibronectin (FN) and examine the role of EDA+ FN in the pathogenesis of rheumatoid joint lesions.Methods. Localization of EDA+ FN and c‐Fos protein in rheumatoid joints was studied immunohistochemically by utilizing antibodies for EDA+ FN and c‐Fos. Expression of EDA+ FN was studied by immunoelectron microscopy and in situ hybridization. The amount of EDA+ FN was measured by enzymelinked immunosorbent assay.Results. EDA+ FN was specifically localized in the synovial lining layer of synovium with active rheumatoid arthritis (RA) (n = 17), but not in that with osteoarthritis (n = 4) or with inactive fibrous RA (n = 2). EDA+ FN messenger RNA was localized in the synovial lining layer. EDA+ FN was immunoelectron microscopically localized in the synovial lining fibroblast‐like (type B) cells. EDA+ FN was also detected at the cartilage–pannus junction and on the surface of RA cartilage. Double staining showed that EDA+ FN colocalized with c‐Fos protein in the rheumatoid synovial lining layer. Quantification of EDA+ FN showed that it was highly concentrated in rheumatoid synovial fluids.Conclusion. EDA+ FN is synthesized by the synovial lining fibroblast‐like (type B) cells in situ in rheumatoid synovium, and appears to be expressed in association with activated or transformed states of synovium.
Identification of human t cell leukemia/lymphoma virus type i antibodies, dna, and protein in patients with polymyositis
Authors: Sherman Michael P. , Amin Rajnikant M. , Rodgers‐Johnson Pamela E. B. , Morgan Owen S. C. , Char Gurendra , Mora Carlos A. , Iannone Robert , Collins George H. , Papsidero Lawrence , Gibbs Clarence J. , Poiesz Bernard J. 
Published: February 2007

AbstractObjective. To investigate a possible association between human T cell leukemia/lymphoma virus type I (HTLV‐I) and polymyositis (PM).Methods. Sera and muscle biopsy samples from 9 Jamaican PM patients were compared with specimens from American HTLV‐I–positive PM patients and normal controls. Sera were evaluated for HTLV antibodies by enzyme‐linked immunosorbent assay and Western blot. The biopsy samples were analyzed for HTLV‐I/II DNA by polymerase chain reaction and were also immunohistochemically stained for HTLV gp46 envelope protein.Results. Seven of the 8 Jamaican PM patients from whom sera were available were HTLV‐I seropositive. The muscle biopsies of all 9 Jamaican patients demonstrated severe lymphocytic infiltration, cellular degeneration, myofiber atrophy, and fibrosis. Each muscle biopsy specimen contained HTLV‐I DNA. Two of 6 samples demonstrated intense staining for HTLV‐I gp46 in many of the invading mononuclear cells and weak staining for HTLV‐I gp46 in many of the invading mononuclear cells and weak staining in the adjacent myocytes. Two other specimens were weakly positive for gp46 in rare mononuclear cells. All control specimens were negative for the presence of HTLV‐I DNA and protein.Conclusion. HTLV‐I is associated with an inflammatory muscle disease characterized by direct invasion of the affected muscle by HTLV‐I–infected mononuclear cells.
Increased levels of substance p and calcitonin gene‐related peptide in rat adjuvant arthritis: combined immunohistochemical and radioimmunoassay analysis
Authors: Ahmed Mahmood , Bjurhol Andres , Schultzberg Marianne , Theodorsson Elvar , Kreicbergs Andris 
Published: February 2007

AbstractObjective. To analyze the occurrence of substance P (SP) and calcitonin gene–related peptide (CGRP) in ankle joints and corresponding dorsal root ganglia (L2–L6) of rats with adjuvant arthritis.Methods. Arthritis was induced by inoculation with heat‐killed mycobacteria. The morphologic distribution of SP and CGRP was assessed by immunohistochemical analysis. Tissue concentrations of the neuropeptides were determined by radioimmunoassay.Results. Neuronal CGRP‐like immunoreactivity was clearly increased in the synovium and the dorsal root ganglia, whereas the increase in SP‐positive structures was less pronounced. The tissue concentrations of SP and CGRP were significantly increased both in ankle joints and in dorsal root ganglia.Conclusion. Levels of sensory neuropeptides are increased under conditions of joint inflammation.
A fatal pulmonary complication of lupus in pregnancy
Authors: Rubin Laurence A. , Geran Amir , Rose Toby H. , Cohen Howard 
Published: February 2007

AbstractPulmonary vasculitis is an uncommon complication of systemic lupus erythematosus (SLE), and has not been previously documented in pregnancy. This case report describes the rapidly fatal course of a pregnant SLE patient, whose terminal illness was manifested by severe pulmonary hypertension and unexpected autopsy findings of medium and large vessel vasculitis. The relationship of these unusual manifestations to other clinical and serologic features of SLE, as well as novel therapeutic options, are discussed.
Expression of e‐selectin messenger RNA and protein in rheumatoid arthritis
Authors: Kriegsmann Jörg , Keyszer Gernot M. , Geiler Thomas , Lagoo Anand S. , Lagoo‐Deenadayalan Sandhya , Gay Renate E. , Gay Steffen 
Published: February 2007

AbstractObjective. To examine the de novo synthesis and cellular distribution of the E‐selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA).Methods. Immunohistochemistry techniques combined with in situ hybridization were used to examine RA synovium.Results. There were numerous endothelial cells positive for E‐selectin and E‐selectin messenger RNA in the RA synovial membranes. Moreover, E‐selectin expression appeared to correlate with inflammatory activity.Conclusion. The strong vascular expression of E‐selectin indicates an activation of endothelial cells in the recruitment of cells associated with the chronic inflammation of RA.
Correlation between gold‐induced enterocolitis and the presence of the HLA‐DRB 1*0404 allele
Authors: Evron Ella , Brautbar Chaim , Becker Stuart , Fenakel Gavriel , Abend Yitzhak , Sthoeger Zev , Cohen Patrizia , Geltner David 
Published: February 2007

AbstractObjective. In recent years we have treated 4 rheumatoid arthritis (RA) patients who developed goldinduced enterocolitis, a well‐recognized, although rare, complication of chrysotherapy. The aim of the present study was to seek any genetic predisposition for this complication.Methods. HLA DNA typing was done on fresh white blood cells from the 4 patients.Results. Three of the 4 patients (75%) exhibited the DRB 1*0404 allele, whereas the prevalence of this allele among the Ashkenazi Jewish population of RA patients without colitis was 9.2% and 10.2% in 2 different studies.Conclusion. The results indicate that the DRB 1*0404 may be associated with risk for the development of gold‐induced enterocolitis in this population and suggest that HLA DNA typing should be considered in Jews who may be undergoing chrysotherapy.