bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative proteomic expression profiling associations with resistance to onion-Fusarium basal rot
Authors: Poursakhi SaeidReza , Asadi-Gharneh Hossein Ali , Esfahani Mehdi Nasr , abbasi Zahra , Khankahdani Hamed Hassanzadeh 
Published: March 2024

Abstract
Fusarium basal rot (FBR) is a serious disease of onion that causes significant losses globally. There are several control measures, of which planting of resistant FBR-cultivars is the most advantageous control option. Thus, to elucidate the involving mechanisms to FBR infection, we consequently carried out quantification changes in antioxidant enzymes activity including ROS (reactive oxygen species); superoxide dismutase (SOD), peroxidase (POX), and catalase (CAT) in two resistant onion genotypes ‘Saba’ and ‘Saba – HS’; and susceptible ‘Savannah Sweet’ and ‘Golden eye’ following inoculation with FBR agent. As a complimentary to the related antioxidant enzymes, we also characterized the critical transcription factors (TFs); R1, R5 and RGA29 marker genes using qRT-PCR involved in endowing resistance at three time course/week post inoculation (wpi). Additionally, we analyzed the changes in biomass growth parameters (BGPs) accordingly. The results showed that the lowest disease severity percentage (DSP) was in resistant ‘Saba’ and ‘Saba – HS’ (6.7 and 4.7 %), and the highest ones in susceptible ‘Savannah Sweet’ and ‘Golden eye’ (88.9 and 89.6%), respectively. There was also a significant increase in CAT, POX, and SOD activities in resistant genotypes. The highest CAT activity was recorded in resistant ‘Saba’- 4.91, and Saba-HS - 3.75 fold, followed by the same trend relatively for POX (‘Saba’- 3.53 and ‘Saba-HS’- 2.35 fold); and SOD (‘Saba’ 14.46 -fold and ‘Saba-HS’ 12.95 –fold) significantly. It was also found that FOC could up-regulate the defense marker genes; R1, PR5, and RGA29 expression in resistant '‘Saba’' and ‘Saba’-HS ranging from 1.23 to 5.01-fold. The highest regulation was in PR5-5.01 fold followed by R1-4.78; and RGA29-3.83-fold, respectively. In addition, BGPs displayed almost similar trends as was in enzyme and gene expression accordingly. These findings lead to introduction of newly FBR-resistant onion genotypes, and also can be implicated in integrated pest management planning programs against FBR disease. Cumulatively, the proteomic-transcriptomic-data provide novel-insights for improving onion-breeding programs against FBR disease.
Tertiary lymphoid structure was a predictor of favorable prognosis in muscle-invasive bladder cancer
Authors: Teng Xiaodong , Chen Zhen , Bai Yanfeng , Cao Hui , Zhang Jing , Xu Liming , Liu Kaihua , Shi Yuqian , Shao Yang 
Published: March 2024

Abstract

Background: Tertiary lymphoid structure (TLS) has been reported to be associated with prognosis and immunotherapy in certain cancers. In this study, we aimed to explore the prognostic role of TLS in Muscle-invasive bladder cancer (MIBC), and to analyze the clinicopathological and molecular factors affecting the formation of TLS.
Methods: Immunohistochemistry was used to detect the expression of TLS, CD8+ T cells, B cells, and plasma cells in 119 MIBC cases, of which 80 cases were tested by next generation sequencing (NGS) for analysising the differences in gene alterations between TLS-negative and TLS-positive.
Results:TLSs were present in 52.1% of MIBC cases. Patients with TLS had lower T and TNM stages, and had longer overall survival (OS) than patients without TLS. Multivariate analysis showed that TLS was an independent prognostic factor. The densities of B cells, CD8+ T cells, and plasma cells in tumors were significantly correlated with TLS, but in the cases with low density B cells, high density CD8+ T cells or high density plasma cells, differences in OS between the tumors with TLS and without TLS were not significant. Compared with TLS-negative tumors, TLS-positive tumors had lower frequency of TP53mutations and higher frequencies of FAT1, CDKN1A mutations. Tumor mutational burden (TMB)was not significantly different between the two groups, but was significantly associated with TLS in TP53 wild-type tumors.
Conclusions:TLS was an independent predictor of favorableprognosis in MIBC, and mainly played antitumor role effect through B cells. TP53mutations could inhibit the formation of TLS.
Failure mechanism of cement plug-formation interface under the thermo- mechanical coupling effect in old wellbore for salt cavern gas storage
Authors: Wang Jiangshuai , Cai chuchu , Fu Pan , Wang Hongjie , Yu Guijian , Deng Song , Tang Zheng 
Published: March 2024

Abstract

The effective sealing of old wells previously used for brine extraction is a key task for the transformation of salt caverns into gas storage tanks and their safe and long-term operation. The essence of the work is to explore the failure problem of the cement plug formation interface in the sealing section. In this paper, a three-dimensional finite element model of cement plug-formation was developed under the influence of mechanical and thermal coupling. This model was used to clarify the enhancement mechanism of thermal effect on the in-situ bonding of the interface. And, the effects of gas storage operation parameters, sealing section formation characteristics, and cement plug body parameters on the ultimate stripping length of the cement plug were studied. Moreover, the ultimate peel failure length of the interface under the coupling effect of multiple factors was determined. The research results indicate that under the influence of temperature and pressure conditions, the formation undergoes a phenomenon of radial shrinkage, increasing the contact stress between the cement plug and the formation, resulting in a denser cementation between the cement plug and the formation interface, thereby reducing the detachment limit.
Evolution of electrical resistance of graphite foils during spark plasma sintering.
Authors: Mwaro William B. , Mphahlele Mahlatse R. , Walker Mark 
Published: March 2024

Abstract
The progression of the electrical resistance of graphite foils during spark plasma sintering process (SPS) was investigated at constant temperature and pressure. The study applied various set-ups of the SPS device, and the electrical data used for the evaluation of electrical resistance (heating power and current) was obtained from the SPS apparatus in real-time. The contact resistance and resistance due to graphite foil/s was evaluated by subtracting the resistance of the single punch set-up from the set-up of two punches in direct contact and the set-ups with various graphite foils. The results showed that during the initial stages of sintering, set-up resistance increases with time and that, overall, set-up resistance increases with number of graphite foils. Both contact resistance and resistance due to graphite foils was found to decrease with sintering time. In contrast to previous conceptions, the electrical resistance of graphite foils changes in response to sintering conditions during the SPS process.
Study on the balance and gait characteristics of patients with generalized joint hypermobility in high- altitude areas using wearable devices
Authors: Liu mingwei , Guo Luqi , Lin Jinpeng , Cai Yuepeng , Huang Xiaofan , Wu Yue , Zhang Yu , Wang Shaobai 
Published: March 2024

Abstract

PURPOSE
To explore the balance and gait function characteristics of Generalized Joint Hypermobility (GJH) patients in high-altitude environments.
METHODS
In this study, 61 university students (GJH: 28, healthy: 33) were recruited in Linzhi City, Tibet Autonomous Region (TAR). An intelligent rehabilitation assessment and training system (Riablo™) was used to assess the static balance with eyes opened and closed, and gait function with flat walking of the two groups of subjects.
RESULTS
Compared with healthy subjects, GJH subjects showed significantly impaired balance, reflected by increased distance of center of pressure position from the ideal center of gravity (SWAY) and amplitude of center of pressure sagittal plane displacements (RMS). Gait velocity, stride length, and swing stance of gait cycle were significantly smaller compared to the healthy subjects. A significant increasement was found for flat-foot of gait cycle compared to the control subjects.
CONCLUSION
The current study demonstrated that patients with GJH in high-altitude environments exhibit impairments in balance and gait using wearable devices, providing a foundation for training and prevention of GJH in high-altitude environments. At the same time, it also provides a reference for joint detection methods in high-altitude environments.
Trial registration
Controlled Trials No.102772023RT133, Registered 13 October 2023
Construction and validation of a prognostic prediction model for hepatectomy of hepatocellular carcinoma by preoperative fibrinogen to prealbumin ratio（FPR）
Authors: Ling Yuan , Yu Ze-Yan , Zhao Yuan-Quan , Wang Xiao-Bo , Wu Fei-Xiang , Lu Shao-Long , Chen Jie 
Published: March 2024

Abstract
Purpose
To enhance the prognostic prediction of patients following hepatectomy, our study aimed to investigate potential predictors of postoperative prognosis in hepatocellular carcinoma (HCC) patients and develop a nomogram model capable of effectively predicting their postoperative prognosis
Methods
Data from 1056 HCC patients who underwent hepatectomy from January 2014 to 2018 in December were retrospectively studied. The preoperative fibrinogen and prealbumin results were collected from all patients, and the preoperative fibrinogen to prealbumin ratio(FPR)was calculated. The optimal cutoff value method was used to differentiate between the high and low FPR groups, and the nomogram prognostic model of the patients was constructed by combining with the results of the postoperative pathology examination and other results.
Results
According to the best cut-off value, the FPR groups were divided into high and low FPR groups with a cut-off value of 0.019, and the high FPR group was associated with poor prognosis of the patients. Barcelona (BCLC) stage, microvascular invasion (MVI), and pathologic grade were independent predictors after hepatectomy in patients with HCC. Combining FPR and, these factor variables to create a prognostic nomogram, the model was confirmed to have a good fit while showing good performance with C-indexes of 0.736 and 0.754 for the training and validation sets, respectively.
Conclusion
A novel and validated nomogram was constructed by FPR combined with tumor staging and postoperative pathology results for predicting the prognosis of HCC patients after hepatectomy.
Nest predatory behavior in the Great Kiskadee (Pitangus sulphuratus): an overlooked predator in the Neotropical cities?
Authors: Camilotti¹ Vagner L. 
Published: March 2024

Abstract
Bird’s nest predation can have a significant impact on the diversity and structure of bird assemblages in urban ecosystems. Our understanding of this phenomenon in Neotropical cities, however, is still limited. The Great Kiskadee (Pitangus sulphuratus), a common species in urban environments, is known to prey on various vertebrate species, yet there is little information on its predation of birds or their nests. Similar to other well-known nest predators in cities worldwide, the Great Kiskadee exhibits behavioral and ecological traits that suggest it may play an important role in nest predation. Nevertheless, its role in this context has yet to be explored. Through a case report of nest predation on the Purple-throated Euphonia (Euphonia chlorotica) in an urban setting and a review of its ecological and biological traits, this study explores the potential and possibly overlooked role of the Great Kiskadee as a significant nest predator in Neotropical cities.
Physical fitness in patients treated with maintenance dialysis: a cross-sectional study
Authors: Baptista Ana Carolina Pereira , Prado Wagner Luiz , Christofaro Diego Giulliano Destro , Torres Michael , Botero João Paulo 
Published: March 2024

Abstract

Background: Low physical fitness has been associated with deterioration in overall health outcomes, resulting in increased hospitalization, morbidity, and mortality in patients treated with maintenance dialysis. This study aimed to compare self-reported physical fitness between patients treated with maintenance dialysis and those without chronic kidney disease (CKD).
Methods: In this cross-sectional study, patients treated with maintenance dialysis (n=674) and individuals without CKD (control, n=518) self-reported physical fitness by responding to the International Fitness Scale (IFIS). Binary Logistic Regressions were adjusted for sex, age, schooling, diabetes, smoking, physical activity, sedentary behavior, and body max index.
Results: Patients undergoing chronic hemodialysis are more likely to self-report lower general PF (OR=2.03; 95% CI: 1.35 to 3.05; p = 0.001), cardiorespiratory fitness (OR=3.18; 95% CI: 2.27 to 4.46; p<0.001), strength (OR=2.51; 95 % CI: 1.71 to 3.68; p<0.001) and agility (OR=1.83; 95% CI: 1.26 to 2.64; p=0.001) than the control group. Patients undergoing chronic hemodialysis who self-reported higher levels of physical activity were 5.64 times more likely to self-report high overall physical fitness (OR=5.64, 95% CI: 1.93 to 16.45, p<0.002).
Conclusion: Patients treated with maintenance dialysis were twice as likely to self-report lower general physical fitness compared to individuals without kidney disease. Considering the consequences related to these factors, strategies to improve it, like initiatives related to the practice of physical exercises with this population, including strategically during hemodialysis, may be interesting.
Comparative Evaluation of Insulation Cotton Configuration on The Investment Casting Quality of Industrial Valve Parts
Authors: Chen Kao-Yi , Khoiruddin Sukhoiri , Lin Chieh-Fong , Ho Ming-Hsiu , Huang Cheng-Fu , Lee Sheng-Chan , Chan Chien-Wei , Mardiono Intan , Fuh Yiin-Kuen 
Published: March 2024

Abstract

This study contributed to the comparison of insulation cotton configurations for the investment casting quality of industrial valve parts. Due to the complexity, parts of industrial valve bodies are usually produced by casting. However, the investment casting production process has several problems, such as shrinkage porosity. Currently, the investment casting industry uses cotton configuration to solve the problem of shrinkage porosity. Therefore, this study compared three cotton installation treatments in the mould shell to determine the optimal use of cotton. There are three schemes of the type of cotton installation on the shell that will be observed, including the triangular insulation cotton area denoted as Case A installed 55mm and Case B installed 90mm cotton on the right and left sides, and Case C denoted with adding bracket arms on the upper side. In this study, we used RMM (Retained Melt Modulus) and the finite element model to analyze the shrinkage porosity's location and to monitor the porosity that occurred during the solidification process. The result showed that cotton could reduce shrinkage porosity by 3.30%, 1.95%, and 1.47% for Case A, Case B, and Case C, respectively. Therefore, the best alternative is to add the cotton and bracket arm on the upper side, which is the optimal strategy to prevent shrinkage porosity.
Clinical outcomes of aortic root repair using pericardial autograft for acute type A aortic dissection
Authors: Liu Chuang , Wang Yao , Ouyang Peigang , Sun Yangxue , Huang Lingchen , Sun Xiaogang , Qian Xiangyang , Yu Cuntao , Guo Hongwei 
Published: March 2024

Abstract
Background
For acute type A aortic dissection involving the aortic root with root diameter no more than 45mm, there are various aortic root repair techniques. In this study, a novel surgical technique using a pericardial autograft for aortic root repair was introduced. We described its surgical steps in detail and compare its clinical outcomes with direct suture technique.
Methods
Between July 2017 and August 2022, 95 consecutive patients with acute type A aortic dissection who underwent aortic root repair were enrolled, including aortic root repair using pericardial autograft (group A, n = 49) or direct suture (group B, n = 46). The patient’s clinical data were retrospectively analyzed, and a 5-year follow-up was conducted.
Results
The 30-day mortality, reoperation for hemostasis, postoperative new-onset renal failure requiring continuous renal replacement therapy, stroke, and paraplegia occurred in 3%, 4%, 11%, 5%, and 2% of the overall patients, respectively. There was no significant difference in the 30-day mortality and complication rate between the two groups. The 30-day mortality and reoperation for hemostasis marked the primary endpoint events. Logistic regression analysis indicated that there was a significant correlation between the primary endpoint events and surgical technique (odds ratio, 0.002; 95% confidence interval, 0-0.159; P = 0.026). The aortic valve insufficiency of the two groups were significantly improved after operation (group A, P < 0.001; group B, P < 0.001). During follow-up, there was no significant difference in short-term survival between the two groups after surgery (log-rank P = 0.75), and all patients were free from reoperation for aortic disease.
Conclusions
Patients who underwent aortic root repair using pericardial autograft tended to have reduced 30-day mortality and a lower risk of reoperation for hemostasis. Using pericardial autograft for aortic root repair is a safe and useful approach for patients with acute type A aortic dissection involving the aortic root.
Elucidating morphogenic and physiological traits of rice with nitrogen substitution through nano-nitrogen under salt stress conditions
Authors: Kumar Ashwani , Sheoran Parvender , Kumar Naresh , Devi Sunita , Kumar Arvind , Malik Kapil , Rani Manu , Bhardwaj Ajay Kumar , Mann Anita 
Published: March 2024

Abstract

Background
Sustainable crop production along with best nutrient use efficiency is the key indicator of smart agriculture. Under abiotic stress conditions, nutrient uptake and translocation are restricted affecting plant growth and yield. The combined effect of nano-nitrogen and salt stress are very limited. Recent developments in slow releasing, nano-fertilizers in agriculture, begins a new era for sustainable use and management of natural resources. The aim of the study was to explore whether substitution with nano-urea could mitigate the detrimental effects of salt stress on plant growth and development along with physiological traits.
Results
With the onset of nano-formulations, this study was designed to explore the potential of nano-nitrogen by replacing traditional urea applications in 33, 50 and 60% doses on rice plant growth and development. Further, the strategic substitutions were compared in saline and sodic soils along with normal soils to dissect the beneficial response of nano-N in two rice varieties under saline stress of ECe ~ 6.0 dSm− 1 and sodic stress of pH ~ 9.1. With saline and sodic stresses, plant RWC decreased by 10.33% and total chlorophyll content by 1.32–1.52%, gas exchange attributes by 10.42–38.8%, leaf area upto 29.9% and yield upto 3.92%, respectively. Leaf greenness (SPAD index) was significantly higher with Nano-N along with number of grains/panicle, crop growth rate and net assimilation rate. Results depicted superiority of nitrogen replacement through nano-N with 0.2–1.64% enhancement in growth traits, 0.93–1.85% in physiological traits, and 1.92% in yield over 100% recommended dose of urea on cumulative basis in rice. Between varieties, salt tolerant rice variety, CSR-10 performed better than Pusa 1121 in terms of morphological, physiological and yield traits under stress conditions and nitrogen substitutions.
Conclusions
Overall, our experimental findings depicted promising potential of nano-N for enhancing the physiological growth and yield under salt stress and also optimizing better yield in normal soils with 33% replacement of conventional urea.
Plume and wall temperature impact on the subsonic aft-body flow of a generic space launcher geometry
Authors: Kirchheck Daniel , Schumann Jan-Erik , Fertig Markus , Saile Dominik , Hannemann Volker , Eggers Thino , Gülhan Ali 
Published: March 2024

Abstract

Experimental and numerical simulation of launcher base flows is crucial for future launcher design. In experiments, the exhaust plume simulation is often limited to cold or slightly heated gases. In numerical simulations, multi-species reactive flow is often neglected due to limited resources. The impact of these simplifications on the relevant flow features compared to real flight scenarios needs to be characterized in order to enhance the design process. Experimental and numerical investigations were carried out in the frame of the SFB/TRR 40 Collaborative Research Centre in order to study the impact of plume and wall temperature on the base flow of a generic small-scale launcher configuration. Wind tunnel tests were performed in the Hot Plume Testing Facility (HPTF) at DLR Cologne, using subsonic ambient flow and pressurized air or hydrogen-oxygen-combustion as exhaust gases. The tests were numerically rebuilt using the DLR TAU code employing a scale-resolved IDDES approach, including thermal coupling and detailed chemistry. The paper combines the experimental and numerical findings from the SFB/TRR 40 base flow studies and highlights the most prominent influences on the mean flow field, the pressure field, the dynamic wake flow motion and the resulting aerodynamic forces on the nozzle. High-frequency pressure measurements, high-speed Schlieren recordings and time-resolved CFD results are evaluated using spectral and modal analysis of the one- and two-dimensional flow field data.