bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of the fat composition and prices of margarines between 2002 and 2006, when new Canadian labelling regulations came into effect
Authors: Ricciuto Laurie , Lin Kevin , Tarasuk Valerie 
Published: November 2008

AbstractObjectiveTo examine the effect of the new Canadian labelling regulations on the fat composition and prices of margarines.Study designA survey of all margarines sold in major supermarkets in the Greater Toronto area was conducted in 2006, and results were compared with those of a similar survey conducted in 2002. Average fat composition, proportion of ‘trans fat-free’ margarines and average prices of margarines were compared. A general linear model procedure was used to compare the relationship between price and fat composition in 2002 and 2006.ResultsAverage amounts of trans fatty acids (TFA) and MUFA decreased, while average amounts of PUFA increased significantly from 2002 to 2006. The proportion of margarines with less than 0·2 g TFA/10 g serving rose significantly from 31 % in 2002 to 69 % in 2006. Margarines lower in TFA on average cost significantly more than margarines with greater amounts of these fats, and this relationship appeared stronger in 2006 relative to 2002.ConclusionsThere is evidence of reductions in TFA in margarines since new labelling regulations came into effect in Canada; however, TFA reductions appeared to be restricted to higher-priced margarines. Results suggest that voluntary approaches (i.e. manufacturer incentives via labelling) to reduce population intakes of TFA will yield little changes in TFA content of low-cost products and thus may have limited benefit for lower-income groups, who are at higher risk of heart disease.
Two-Photon Imaging of Cellular Activities in Oxygen Sensing Tissues
Authors: Wotzlaw Christoph , Berchner-Pfannschmidt Utta , Fandrey Joachim , Acker Helmut 
Published: November 2008

AbstractThe cellular oxygen sensing system of the body ensures appropriate adaptation of cellular functions toward hypoxia by regulating gene expression and ion channel activity. Two-photon laser microscopy is an ideal tool to study and prove the relevance of the molecular mechanisms within oxygen sensing pathways on the cellular and complex tissue or organ level. Images of hypoxia inducible factor 1 (HIF-1) subunit nuclear mobility and protein-protein interaction in living cells, of hypoxia-induced changes in membrane potential and intracellular calcium of live ex vivo carotid bodies as well as of rat kidney proximal tubulus function in vivo, will be shown.
On the Field Evaporation Behavior of a Model Ni-Al-Cr Superalloy Studied by Picosecond Pulsed-Laser Atom-Probe Tomography
Authors: Zhou Yang , Booth-Morrison Christopher , Seidman David N. 
Published: November 2008

AbstractThe effects of varying the pulse energy of a picosecond laser used in the pulsed-laser atom-probe (PLAP) tomography of an as-quenched Ni-6.5 Al-9.5 Cr at.% alloy are assessed based on the quality of the mass spectra and the compositional accuracy of the technique. Compared to pulsed-voltage atom-probe tomography, PLAP tomography improves mass resolving power, decreases noise levels, and improves compositional accuracy. Experimental evidence suggests that Ni2+, Al2+, and Cr2+ions are formed primarily by a thermally activated evaporation process, and not by post-ionization of the ions in the 1+charge state. An analysis of the detected noise levels reveals that for properly chosen instrument parameters, there is no significant steady-state heating of the Ni-6.5 Al-9.5 Cr at.% tips during PLAP tomography.
Microbial communities associated with the invasive Hawaiian sponge Mycale armata
Authors: Wang Guangyi , Yoon Sang-Hwal , Lefait Emilie 
Published: November 2008

Abstract
Microbial symbionts are fundamentally important to their host ecology, but microbial communities of invasive marine species remain largely unexplored. Clone libraries and Denaturing gradient gel electrophoresis analyses revealed diverse microbial phylotypes in the invasive marine sponge Mycale armata. Phylotypes were related to eight phyla: Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Acidobacteria, Chloroflexi, Crenarchaeota and Firmicutes, with predominant alphaproteobacterial sequences (&gt;58%). Three Bacterial Phylotype Groups (BPG1––associated only with sequence from marine sponges; BPG2––associated with sponges and other marine organisms and BPG3––potential new phylotypes) were identified in M. armata. The operational taxonomic units (OTU) of cluster BPG2-B, belonging to Rhodobacteraceae, are dominant sequences of two clone libraries of M. armata, but constitute only a small fraction of sequences from the non-invasive sponge Dysidea sp. Six OTUs from M. armata were potential new phylotypes because of their low sequence identity with their reference sequences. Our results suggest that M. armata harbors both sponge-specific phylotypes and bacterial phylotypes from other marine organisms.
Dielectric Constant of Water Discovered to be Simple Function of Density over Extreme Ranges from – 35 to + 600^o^C and to 1200 MPa (12000 Atm.), Believed Universal
Authors: Marshall William L. 
Published: November 2008

AbstractThe experimental static dielectric constants ([epsilon]) of water formulated by Fernández, et al., (1997-International Association for the Properties of Water and Steam, IAPWS), –35 to 600^o^C; 0.1 to 1200 MPa, are discovered to be closely described throughout by a simple equation of density ([rho]), presented here, and agreeing with their reported calculated (extrapolated) values to 923^o^C. Eight limiting boundary conditions are observed. Values of [epsilon] follow isothermal straight-line slopes of log ([epsilon] – 1) vs log [rho] down to densities of 0.25 g cm^-3^ water, even in dioxane-water solutions. At lower densities, all slopes curve asymptotically in approaching unity, while becoming unity throughout at –35^o^C (near metastable freezing point). Slopes above 0.25 g cm^-3^ asymptotically approach 1 2/3 at high temperatures that numerically proportionates ([epsilon] – 1) to volume times surface densities, with a proposed explanation given. Values for [epsilon] of water dissolved in dioxane down to 0.006 g cm^-3^ water (25-300^o^C) and in benzene to 0.014 g cm^-3^ water (300-400^o^C) extend uninterrupted from those for pure water. The Deul-Franck (1984, 1991) values of [epsilon] for benzene (25-400^o^C; to 400 MPa) by the relationship fall on a single straight-line independent of temperature. Theorists should evaluate this simple relationship of water and other liquids to density that appears to be universal.
Mediators of mineralocorticoid receptor-induced profibrotic inflammatory responses in the heart
Authors: Wilson Peter , Morgan James , Funder John W. , Fuller Peter J. , Young Morag J. 
Published: November 2008

Coronary, vascular and perivascular inflammation in rats following MR (mineralocorticoid receptor) activation plus salt are well-characterized precursors for the appearance of cardiac fibrosis. Endogenous corticosterone, in the presence of the 11βHSD2 (11β hydroxysteroid dehydrogenase type 2) inhibitor CBX (carbenoxolone) plus salt, produces similar inflammatory responses and tissue remodelling via activation of MR. MR-mediated oxidative stress has previously been suggested to account for these responses. In the present study we thus postulated that when 11βHSD2 is inhibited, endogenous corticosterone bound to unprotected MR in the vessel wall may similarly increase early biomarkers of oxidative stress. Uninephrectomized rats received either DOC (deoxycorticosterone), CBX or CBX plus the MR antagonist EPL (eplerenone) together with 0.9% saline to drink for 4, 8 or 16 days. Uninephrectomized rats maintained on 0.9% saline for 8 days served as controls. After 4 days, both DOC and CBX increased both macrophage infiltration and mRNA expression of the p22phox subunit of NADPH oxidase, whereas CBX, but not DOC, increased expression of the NOX2 (gp91phox) subunit. eNOS [endothelial NOS (NO synthase)] mRNA expression significantly decreased from 4 days for both treatments, and iNOS (inducible NOS) mRNA levels increased after 16 days of DOC or CBX; co-administration of EPL inhibited all responses to CBX. The responses characterized over this time course occurred before measurable increases in cardiac hypertrophy or fibrosis. The findings of the present study support the hypothesis that endogenous corticosterone in the presence of CBX can activate vascular MR to produce both inflammatory and oxidative tissue responses well before the onset of fibrosis, that the two MR ligands induce differential but overlapping patterns of gene expression, and that elevation of NOX2 subunit levels does not appear necessary for full expression of MR-mediated inflammatory and fibrogenic responses.
Spatial variability of seabird distribution associated with environmental factors: a case study of marine Important Bird Areas in the Azores
Authors: Amorim Patrícia , Figueiredo Miguel , Machete Miguel , Morato Telmo , Martins Ana , Serrão Santos Ricardo 
Published: November 2008

Abstract
Amorim, P., Figueiredo, M., Machete, M., Morato, T., Martins, A., and Serrão Santos, R. 2009. Spatial variability of seabird distribution associated with environmental factors: a case study of marine Important Bird Areas in the Azores. – ICES Journal of Marine Science, 66: 29–40. The spatial structure and distribution at sea of Cory's shearwaters (Calonectris diomedea borealis), common terns (Sterna hirundo), and roseate terns (Sterna dougallii) were analysed in the Azores for various environmental factors: sea surface temperature, chlorophyll a concentration, distance to fronts, wind, distance to island shore or tern colonies, distance to seamounts, seabed slope, and depth. Data on seabird sightings were collected by observers on board fishing vessels, 2002–2006. Generalized linear modelling (GLM) explained 43 and 11% of the abundance variability for terns (both species pooled) and Cory's shearwaters, respectively. Variability in seabird abundance was mainly explained by month, wind, distance to shore and/or tern colonies, and distance to seamounts. Variogram modelling indicated that species distribution presented a small-scale spatial structure (i.e. low autocorrelation). Cory's shearwater predictive distribution maps showed widespread distribution patterns of abundance, despite occurring at a greater intensity around the islands and around some seamounts, which are areas of fishery interest. Conversely, terns were essentially concentrated near the shore. The establishment of marine important bird areas should be encouraged close to seabird colonies and around some seamount areas.
GRASSIUS: A Platform for Comparative Regulatory Genomics across the Grasses
Authors: Yilmaz Alper , Nishiyama Milton Y. , Fuentes Bernardo Garcia , Souza Glaucia Mendes , Janies Daniel , Gray John , Grotewold Erich 
Published: November 2008

Abstract
Transcription factors (TFs) are major players in gene regulatory networks and interactions between TFs and their target genes furnish spatiotemporal patterns of gene expression. Establishing the architecture of regulatory networks requires gathering information on TFs, their targets in the genome, and the corresponding binding sites. We have developed GRASSIUS (Grass Regulatory Information Services) as a knowledge-based Web resource that integrates information on TFs and gene promoters across the grasses. In its initial implementation, GRASSIUS consists of two separate, yet linked, databases. GrassTFDB holds information on TFs from maize (Zea mays), sorghum (Sorghum bicolor), sugarcane (Saccharum spp.), and rice (Oryza sativa). TFs are classified into families and phylogenetic relationships begin to uncover orthologous relationships among the participating species. This database also provides a centralized clearinghouse for TF synonyms in the grasses. GrassTFDB is linked to the grass TFome collection, which provides clones in recombination-based vectors corresponding to full-length open reading frames for a growing number of grass TFs. GrassPROMDB contains promoter and cis-regulatory element information for those grass species and genes for which enough data are available. The integration of GrassTFDB and GrassPROMDB will be accomplished through GrassRegNet as a first step in representing the architecture of grass regulatory networks. GRASSIUS can be accessed from www.grassius.org.
The Role of Plasma Membrane Intrinsic Protein Aquaporins in Water Transport through Roots: Diurnal and Drought Stress Responses Reveal Different Strategies between Isohydric and Anisohydric Cultivars of Grapevine
Authors: Vandeleur Rebecca K. , Mayo Gwenda , Shelden Megan C. , Gilliham Matthew , Kaiser Brent N. , Tyerman Stephen D. 
Published: November 2008

AbstractWe report physiological and anatomical characteristics of water transport across roots grown in soil of two cultivars of grapevine (Vitis vinifera) differing in response to water stress (Grenache, isohydric; Chardonnay, anisohydric). Both cultivars have similar root hydraulic conductances (L o; normalized to root dry weight) that change diurnally. There is a positive correlation between L o and transpiration. Under water stress, both cultivars have reduced minimum daily L o (predawn) attributed to the development of apoplastic barriers. Water-stressed and well-watered Chardonnay had the same diurnal change in amplitude of L o, while water-stressed Grenache showed a reduction in daily amplitude compared with well-watered plants. Hydraulic conductivity of root cortex cells (L pcell) doubles in Chardonnay but remains unchanged in Grenache. Of the two most highly expressed plasma membrane intrinsic protein (PIP) aquaporins in roots (VvPIP1;1 and VvPIP2;2), only VvPIP2;2 functions as a water channel in Xenopus laevis oocytes. VvPIP1;1 interacts with VvPIP2;2 to induce 3-fold higher water permeability. These two aquaporins are colocated in the root from in situ hybridization and immunolocalization of VvPIP1 and VvPIP2 subfamily members. They occur in root tip, exodermis, root cortex (detected up to 30 mm), and stele. VvPIP2;2 mRNA does not change diurnally or with water stress, in contrast to VvPIP1;1, in which expression reflects the differences in L o and L pcell between cultivars in their responses to water stress and rewatering. VvPIP1;1 may regulate water transport across roots such that transpirational demand is matched by root water transport capacity. This occurs on a diurnal basis and in response to water stress that corresponds to the difference in drought tolerance between the cultivars.
Flux Balance Analysis of Barley Seeds: A Computational Approach to Study Systemic Properties of Central Metabolism
Authors: Grafahrend-Belau Eva , Schreiber Falk , Koschützki Dirk , Junker Björn H. 
Published: November 2008

AbstractThe accumulation of storage compounds is an important aspect of cereal seed metabolism. Due to the agronomical importance of the storage reserves of starch, protein, and oil, the understanding of storage metabolism is of scientific interest, with practical applications in agronomy and plant breeding. To get insight into storage patterning in developing cereal seed in response to environmental and genetic perturbation, a computational analysis of seed metabolism was performed. A metabolic network of primary metabolism in the developing endosperm of barley (Hordeum vulgare), a model plant for temperate cereals, was constructed that includes 257 biochemical and transport reactions across four different compartments. The model was subjected to flux balance analysis to study grain yield and metabolic flux distributions in response to oxygen depletion and enzyme deletion. In general, the simulation results were found to be in good agreement with the main biochemical properties of barley seed storage metabolism. The predicted growth rate and the active metabolic pathway patterns under anoxic, hypoxic, and aerobic conditions predicted by the model were in accordance with published experimental results. In addition, the model predictions gave insight into the potential role of inorganic pyrophosphate metabolism to maintain seed metabolism under oxygen deprivation.
The Arabidopsis A4 Subfamily of Lectin Receptor Kinases Negatively Regulates Abscisic Acid Response in Seed Germination
Authors: Xin Zeyu , Wang Anyou , Yang Guohua , Gao Peng , Zheng Zhi-Liang 
Published: November 2008

AbstractAbscisic acid (ABA) is an important plant hormone for a wide array of growth and developmental processes and stress responses, but the mechanism of ABA signal perception on the plasma membrane remains to be dissected. A previous GeneChip analysis revealed that a member of the A4 subfamily of lectin receptor kinases (LecRKs) of Arabidopsis (Arabidopsis thaliana), At5g01540 (designated LecRKA4.1), is up-regulated in response to a low dose of ABA in the rop10-1 background. Here, we present functional evidence to support its role in ABA response. LecRKA4.1 is expressed in seeds and leaves but not in roots, and the protein is localized to the plasma membrane. A T-DNA knockout mutant, lecrka4.1-1, slightly enhanced ABA inhibition of seed germination. Interestingly, LecRKA4.1 is adjacent to two other members of the A4 subfamily of LecRK genes, At5g01550 (LecRKA4.2) and At5g01560 (LecRKA4.3). We found that loss-of-function mutants of LecRKA4.2 and LecRKA4.3 exhibited similarly weak enhancement of ABA response in seed germination inhibition. Furthermore, LecRKA4.2 suppression by RNA interference in lecrka4.1-1 showed stronger ABA inhibition of seed germination than lecrka4.1-1, while the response to gibberellic acid was not affected in lecrka4.1-1 and lecrka4.1-1; LecRKA4.2 (RNAi) lines. Expression studies, together with network-based analysis, suggest that LecRKA4.1 and LecRKA4.2 regulate some of the ABA-responsive genes. Taken together, our results demonstrate that the A4 subfamily of LecRKs has a redundant function in the negative regulation of ABA response in seed germination.
AucsiaGene Silencing Causes Parthenocarpic Fruit Development in Tomato
Authors: Molesini Barbara , Pandolfini Tiziana , Rotino Giuseppe Leonardo , Dani Valeria , Spena Angelo 
Published: November 2008

AbstractIn angiosperms, auxin phytohormones play a crucial regulatory role in fruit initiation. The expression of auxin biosynthesis genes in ovules and placenta results in uncoupling of tomato (Solanum lycopersicum) fruit development from fertilization with production of parthenocarpic fruits. We have identified two newly described genes, named Aucsia genes, which are differentially expressed in auxin-synthesis (DefH9-iaaM) parthenocarpic tomato flower buds. The two tomato Aucsia genes encode 53-amino-acid-long peptides. We show, by RNA interference-mediated gene suppression, that Aucsia genes are involved in both reproductive and vegetative plant development. Aucsia-silenced tomato plants exhibited auxin-related phenotypes such as parthenocarpic fruit development, leaf fusions, and reflexed leaves. Auxin-induced rhizogenesis in cotyledon explants and polar auxin transport in roots were reduced in Aucsia-silenced plants compared with wild-type plants. In addition, Aucsia-silenced plants showed an increased sensitivity to 1-naphthylphthalamic acid, an inhibitor of polar auxin transport. We further prove that total indole-3-acetic acid content was increased in preanthesis Aucsia-silenced flower buds. Thus, the data presented demonstrate that Aucsia genes encode a novel family of plant peptides that control fruit initiation and affect other auxin-related biological processes in tomato. Aucsia homologous genes are present in both chlorophytes and streptophytes, and the encoded peptides are distinguished by a 16-amino-acid-long (PYSGXSTLALVARXSA) AUCSIA motif, a lysine-rich carboxyl-terminal region, and a conserved tyrosine-based endocytic sorting motif.
Multi‐gene phylogeny of the pantropical subfamily Chrysophylloideae (Sapotaceae): evidence of generic polyphyly and extensive morphological homoplasy
Authors: Swenson Ulf , Richardson James E. , Bartish Igor V. 
Published: November 2008

AbstractWe present a molecular phylogeny of 26 out of the 28 currently accepted genera in the subfamily Chrysophylloideae (Sapotaceae) using parsimony, parsimony jackknifing, and Bayesian inference. A data matrix of 8984 characters was obtained from DNA sequences of seven chloroplast loci, two nuclear loci, indels coded as binary characters, and morphology. Our phylogenetic reconstruction suggests that Chrysophyllum, Pouteria, and Pradosia, as well as some sections within Chrysophyllum and Pouteria, are all polyphyletic. These taxa were previously described largely on the basis of unique combinations of states for a set of morphological characters. Mapping some of these characters onto one of the most parsimonious trees indicates that the symplesiomorphic flower in the subfamily was probably 5‐merous, had stamens inserted in the tube orifice, staminodes, seeds with foliaceous cotyledons, exserted radicle, and endosperm. These characters have subsequently been lost multiple times and cannot be used as synapomorphies to support broad generic concepts. Despite the high degree of homoplasy some well‐defined clades can be described on the basis of alternative character state combinations. Also, many of these well‐supported clades appear to be restricted to particular geographical areas (e.g. all taxa in Australasia form a monophyletic group). Hence, we suggest that the segregate genera Aningeria, Malacantha, and Martiusella may ultimately be resurrected, and probably also Donella and Gambeya, but their circumscriptions are still unclear. One species, Chrysophyllum cuneifolium, may have originated from a hybridization event between continents where the maternal genome (cpDNA) comes from South America and the nuclear genome comes from Africa.© The Willi Hennig Society 2008.
A randomized trial of short psychotherapy versus sustained‐release bupropion for smoking cessation
Authors: Zernig Gerald , Wallner Reinhild , Grohs Ursula , Kriechbaum Norbert , Kemmler Georg , Saria Alois 
Published: November 2008

ABSTRACTAims To compare the efficacy and safety of a novel psychological intervention for smoking cessation called psychodynamic model (PDM) training to an active control condition of sustained‐release bupropion.Design Randomized controlled clinical trial with allocation concealment.Setting Private psychiatric practice.Participants Seven hundred and seventy‐nine adult smokers recruited by advertising.Interventions PDM training (n = 366 participants) consisted of a very brief (1.5 days) psychoeducation and a supervised training in autosuggestion techniques (guided imageries) aimed at enhancing self‐management, decidedness, assertiveness, security and competence in relationships, natural functions of organs and awareness of bodily functions. Bupropion SR (n = 413) was increased to 150 mg twice daily over 1 week and given over a 8‐week period.Measurements Twelve‐month continuous abstinence confirmed by exhaled carbon monoxide (CO) of 9 parts per million (p.p.m.) or less at all interviews conducted at 3, 6 and 12 months.Findings Intention‐to‐treat analysis revealed Russell standard 12‐month continuous abstinence rates of 39.1% in the psychotherapy group versus 12.3% in the bupropion SR group (P < 0.001) with a relative benefit (RB) of 3.16 (2.38–4.26). Completer analysis revealed 12‐month continuous abstinence rates of 39.9% in the psychotherapy group versus 22.5% in the bupropion group [P < 0.001; RB 1.78 (1.35–2.34)]. Of note, bupropion abstinence rates were comparable to previous medications/placebo‐only comparisons in geographically different samples.Conclusions The 1.5‐day psychotherapy exceeded bupropion's efficacy, presenting an alternative to pharmacological smoking cessation aids, especially for smokers who reject drugs to treat their substance dependence, at a similar cost (€350) as the bupropion treatment (€355).