bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Spectacular Undular Bore in Iowa on 2 October 2007
Authors: Coleman Timothy A. , Knupp Kevin R. , Herzmann Daryl 
Published: July 2008

Abstract
A visually impressive undular bore moved across much of Iowa on 2 October 2007, and video animations were captured by numerous Webcams. The bore was sampled very well by Doppler radar at close range, and also by the high-density mesoscale network of surface stations in place over Iowa and 1-min Automated Surface Observing System (ASOS) surface data at Des Moines, Iowa. Radar and surface observations are presented, along with a brief analysis of the structure of the bore.
Regional Ensemble Forecasts Using the Ensemble Transform Technique
Authors: Bishop Craig H. , Holt Teddy R. , Nachamkin Jason , Chen Sue , McLay Justin G. , Doyle James D. , Thompson William T. 
Published: July 2008

AbstractA computationally inexpensive ensemble transform (ET) method for generating high-resolution initial perturbations for regional ensemble forecasts is introduced. The method provides initial perturbations that (i) have an initial variance consistent with the best available estimates of initial condition error variance, (ii) are dynamically conditioned by a process similar to that used in the breeding technique, (iii) add to zero at the initial time, (iv) are quasi-orthogonal and equally likely, and (v) partially respect mesoscale balance constraints by ensuring that each initial perturbation is a linear sum of forecast perturbations from the preceding forecast. The technique is tested using estimates of analysis error variance from the Naval Research Laboratory (NRL) Atmospheric Variational Data Assimilation System (NAVDAS) and the Navy’s regional Coupled Ocean–Atmosphere Mesoscale Prediction System (COAMPS) over a 3-week period during the summer of 2005. Lateral boundary conditions are provided by a global ET ensemble. The tests show that the ET regional ensemble has a skillful mean and a useful spread–skill relationship in mass, momentum, and precipitation variables. Diagnostics indicate that ensemble variance was close to, but probably a little less than, the forecast error variance for wind and temperature variables, while precipitation ensemble variance was significantly smaller than precipitation forecast error variance.
Radiative Impact of Ozone on Temperature Predictability in a Coupled Chemistry–Dynamics Data Assimilation System
Authors: de Grandpré J. , Ménard R. , Rochon Y. J. , Charette C. , Chabrillat S. , Robichaud A. 
Published: July 2008

Abstract
The objective of this study is to investigate the impact on temperature forecast of using ozone analyses for the computation of heating rates in a three-dimensional variational data assimilation (3D-Var) system with a coupled model. The system is based on a tropospheric–stratospheric forecast model that includes a comprehensive stratospheric chemistry module for online resolution of the dynamical, radiative, and photochemical interactions. The system assimilates conventional observations as well as temperature and ozone measurements from the Michelson Interferometer for Passive Atmospheric Sounding (MIPAS) instrument. Several data assimilation cycles have been performed over the period August–October 2003 to produce a set of analyses that have been used for launching an ensemble of 10-day forecasts. Temperature and ozone forecasts have been compared with MIPAS and radiosonde observations in different regions. Results show that, in the absence of ozone assimilation, the impact of using a prognostic ozone distribution for the computation of heating rates as opposed to monthly mean climatologies is generally neutral. With the addition of ozone assimilation, the improvement against a noninteractive assimilation system is systematic and occurs over a wide range of time scales throughout the lower stratosphere. The improvement on 6-h temperature forecasts is mainly seen in the Southern Hemisphere, where ozone analyses are in good agreement with observations. For 10-day forecasts, the impact of using ozone analyses is more important in the Northern Hemisphere, where it improves the temperature predictability by more than 1 day at 50 hPa. Comparisons with analyses also show a systematic reduction of the temperature root-mean-square errors and biases throughout the assimilation period. The overall results demonstrate that a comprehensive coupled 3D-Var system that incorporates the radiative feedback from ozone analyses can be used for improving temperature predictability throughout the stratosphere. Comprehensive approaches can be used as a benchmark for the development of linearized methods for improving temperature and ozone forecasting in the region.
Clay minerals in late Quaternary sediments from the south Chilean margin as indicators of provenance and palaeoclimate
Authors: Marinoni L. , Setti M. , Salvi C. , López-Galindo A. 
Published: July 2008

AbstractAn investigation of bulk-rock and clay-fraction compositions of two sedimentary cores from southern Chile was performed to evaluate the record of temporal climatic changes during the late Quaternary (11 ky and 30 ky BP). The bulk mineralogy shows an abundance of feldspars, mica and quartz, with lesser chlorite, amphibole and pyroxene, and variable amounts of carbonates. The clay fraction consists of illite, chlorite and scarce smectite. Smectite shows platy morphology, an Al-Fe beidellite chemical composition, and is detrital. Smectite, together with biogenic carbonate, increases in levels diagnostic of warmer phases. Increases in smectite are attributed either to the beginning of chemical weathering, allowed by the glacial retreat, or to ice extension and sea-level variations. Warmer climates also favoured the increase of carbonate productivity. Levels diagnostic of colder phases show a large decrease in carbonate, small amounts of smectite and large amounts of chlorite and mica, as the abundance of glaciers reduced the productivity and prevented chemical weathering.
Acid-sulphate hydrothermal alteration of andesitic tuffs and genesis of halloysite and alunite deposits in the Biga Peninsula, Turkey
Authors: Ece Ö. I. , Schroeder P. A. , Smilley M. J. , Wampler J. M. 
Published: July 2008

AbstractThe Biga Peninsula of NW Turkey is host to six major halloysite deposits in the Go¨nen, Yenice and Balya districts. Mineralization took place in areas of Permian limestone blocks where the Triassic Karakaya Complex is in contact with early Miocene calc-alkaline volcanic rocks. Hypogene halloysite mineralization was controlled by the intersection of minor faults in the vicinity of clay deposits. During the Pleistocene, activity of the North Anatolian Fault (NAF) brought ascending geothermal solutions through the fault zones to the surface, which led to hydrothermal alteration and halloysite formation. N-MORB normalized element values for each halloysite deposit and the volcanic rocks suggest genetic links. Alunite and halloysite were formed in the Turplu area where upwelling hydrothermal waters contained major H2S and SO2acids. Only halloysite mineralization occurred in outflow areas of the same fossil geothermal field.Pyrite and alunite samples from the Turplu deposits have δ34S values of 0.6–1.8% and 4.8–7.9%, respectively, with values for gypsum of 3.1–3.5%. The δ34S values of pyrite suggest that local meteoric waters had partially mixed with the dominant fluid during the closure stage of fossil hydrothermal activities. The range of δD values of halloysite samples from Turplu is –58.4 to –68.6%. The δ18O values for halloysite are in the range 16.7–18.1%. All halloysite deposits in the study areas are either overlying or adjacent to limestone blocks, and these provide excellent drainage for the discharging geothermal waters. Subsurface drainage systems in the karstic environment and the SO2-bearing thermal waters indicate the importance of acidic waters and the continuous leaching of elements in forming relatively pure hydrated halloysite. A steam-heated dissolution-precipitation model is proposed for the occurrence of all halloysite and alunite deposits. Sulphur gases (H2S-SO2) of hypogene origin rose from deep in the fault zone to the surface where they encountered oxygenated groundwater at the water table. The occurrence of H2SO4in this hydrothermal system enhanced the acidity of geothermal waters provoking advanced argillic alteration. Hypogene alunite deposits also have large P2O5contents, suggesting a parent material with a magmatic origin deeper than the alkaline tuffs. Halloysite is a fast-forming metastable precursor to kaolinite.
Human CD25highFoxp3pos regulatory T cells differentiate into IL-17–producing cells
Authors: Koenen Hans J. P. M. , Smeets Ruben L. , Vink Paul M. , van Rijssen Esther , Boots Annemieke M. H. , Joosten Irma 
Published: July 2008

AbstractThe effector T-cell lineage shows great plasticity. Th17 cells are acknowledged to be instrumental in the response against microbial infection, but are also associated with autoimmune inflammatory processes. Here, we report that human regulatory T cells (CD4posCD25highFoxp3posCD127negCD27pos) can differentiate into IL-17–producing cells, when stimulated by allogeneic antigen-presenting cells, especially monocytes, in the presence of rhIL-2/rhIL-15. These regulatory T cell (Treg)–derived IL-17–producing cells showed high expression of the Th17-related transcription factor RORγt and were positively identified by CCR6 expression. This differentiation process was enhanced by exogenous IL-1β, IL-23, and IL-21, whereas IL-6 or TGFβ did not affect the emergence of IL-17–producing cells. The addition of IL-1 receptor antagonist (IL-1Ra), but not anti–IL-23 antibody, reduced IL-17–producing cell numbers. When an histone deacetylase (HDAC) inhibitor trichostatin A (TSA) was evaluated, we found a profound negative effect on the emergence of IL-17–producing cells from Tregs, implying that Treg differentiation into IL-17–producing cells depends on histone/protein deacetylase activity. Thus, the data suggest that epigenetic modification underlies the phenomenon of Treg plasticity here described.
Role of GM-CSF in tolerance induction by mobilized hematopoietic progenitors
Authors: Kared Hassen , Leforban Bertrand , Montandon Ruddy , Renand Amédée , Layseca Espinosa Esther , Chatenoud Lucienne , Rosenstein Yvonne , Schneider Elke , Dy Michel , Zavala Flora 
Published: July 2008

AbstractMechanisms of protection against autoimmune diseases by transplantation of autologous hematopoietic progenitors remain poorly defined. We recently demonstrated that, unlike medullary hematopoietic stem cells (HSCs), mobilized hematopoietic progenitors (HPCs) stimulate peripheral Foxp3+ regulatory T cell (Treg)–expansion through cell-contact activation of Notch signaling and through as yet undetermined soluble factor(s), distinct from TGF-β1. Herein we identified one such soluble factor as granulocyte macrophage–colony stimulating factor (GM-CSF), which is produced at higher levels by HPCs than HSCs and whose neutralization significantly reduces the growth-promoting effect of HPCs on Treg. Treg express a functional GM-CSF receptor α-chain CD116 and proliferate in response to this cytokine independently from IL2. GM-CSF–expanded Treg—like HPC-expanded Treg—display enhanced suppressive capacity relative to control Treg. Hence, mobilized progenitors stimulate Treg expansion both by cell-contact dependent mechanisms and by their production of GM-CSF.
Outcome of alloanergized haploidentical bone marrow transplantation after ex vivo costimulatory blockade: results of 2 phase 1 studies
Authors: Davies Jeff K. , Gribben John G. , Brennan Lisa L. , Yuk Dongin , Nadler Lee M. , Guinan Eva C. 
Published: July 2008

AbstractWe report the outcomes of 24 patients with high-risk hematologic malignancies or bone marrow failure (BMF) who received haploidentical bone marrow transplantation (BMT) after ex vivo induction of alloantigen-specific anergy in donor T cells by allostimulation in the presence of costimulatory blockade. Ninety-five percent of evaluable patients engrafted and achieved full donor chimerism. Despite receiving a median T-cell dose of 29 ×106/kg, only 5 of 21 evaluable patients developed grade C (n = 4) or D (n = 1) acute graft-versus-host disease (GVHD), with only one attributable death. Twelve patients died from treatment-related mortality (TRM). Patients reconstituted T-cell subsets and immunoglobulin levels rapidly with evidence of in vivo expansion of pathogen-specific T cells in the early posttransplantation period. Five patients reactivated cytomegalovirus (CMV), only one of whom required extended antiviral treatment. No deaths were attributable to CMV or other viral infections. Only 1 of 12 evaluable patients developed chronic GVHD. Eight patients survive disease-free with normal performance scores (median follow-up, 7 years). Thus, despite significant early TRM, ex vivo alloanergization can support administration of large numbers of haploidentical donor T cells, resulting in rapid immune reconstitution with very few viral infections. Surviving patients have excellent performance status and a low rate of chronic GVHD.
Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity
Authors: de Moreno de LeBlanc Alejandra , Dogi Cecilia A , Galdeano Carolina Maldonado , Carmuega Esteban , Weill Ricardo , Perdigón Gabriela 
Published: July 2008

AbstractBackgroundMicrobial colonization of the intestine after birth is an important step for the development of the gut immune system. The acquisition of passive immunity through breast-feeding may influence the pattern of bacterial colonization in the newborn. The aim of this work was to evaluate the effect of the administration of a probiotic fermented milk (PFM) containing yogurt starter cultures and the probiotic bacteria strainLactobacillus caseiDN-114001 to mothers during nursing or their offspring, on the intestinal bacterial population and on parameters of the gut immune system.ResultsFifteen mice of each group were sacrificed at ages 12, 21, 28 and 45 days. Large intestines were taken for determination of intestinal microbiota, and small intestines for the study of secretory-IgA (S-IgA) in fluid and the study of IgA+ cells, macrophages, dendritic cells and goblet cells on tissue samples. The consumption of the PFM either by the mother during nursing or by the offspring after weaning modified the development of bifidobacteria population in the large intestine of the mice. These modifications were accompanied with a decrease of enterobacteria population. The administration of this PFM to the mothers improved their own immune system and this also affected their offspring. Offspring from mice that received PFM increased S-IgA in intestinal fluids, which mainly originated from their mother's immune system. A decrease in the number of macrophages, dendritic cells and IgA+ cells during the suckling period in offspring fed with PFM was observed; this could be related with the improvement of the immunity of the mothers, which passively protect their babies. At day 45, the mice reach maturity of their own immune system and the effects of the PFM was the stimulation of their mucosal immunity.ConclusionThe present work shows the beneficial effect of the administration of a PFM not only to the mothers during the suckling period but also to their offspring after weaning and until adulthood. This effect positively improved the intestinal microbiota that are related with a modulation of the gut immune response, which was demonstrated with the stimulation of the IgA + cells, macrophages and dendritic cells.
Insulin gene polymorphisms in type 1 diabetes, Addison's disease and the polyglandular autoimmune syndrome type II
Authors: Ramos-Lopez Elizabeth , Lange Britta , Kahles Heinrich , Willenberg Holger S , Meyer Gesine , Penna-Martinez Marissa , Reisch Nicole , Hahner Stefanie , Seissler Jürgen , Badenhoop Klaus 
Published: July 2008

Abstract

Background
Polymorphisms within the insulin gene can influence insulin expression in the pancreas and especially in the thymus, where self-antigens are processed, shaping the T cell repertoire into selftolerance, a process that protects from β-cell autoimmunity.

Methods
We investigated the role of the -2221Msp(C/T) and -23HphI(A/T) polymorphisms within the insulin gene in patients with a monoglandular autoimmune endocrine disease [patients with isolated type 1 diabetes (T1D, n = 317), Addison's disease (AD, n = 107) or Hashimoto's thyroiditis (HT, n = 61)], those with a polyglandular autoimmune syndrome type II (combination of T1D and/or AD with HT or GD, n = 62) as well as in healthy controls (HC, n = 275).

Results
T1D patients carried significantly more often the homozygous genotype "CC" -2221Msp(C/T) and "AA" -23HphI(A/T) polymorphisms than the HC (78.5% vs. 66.2%, p = 0.0027 and 75.4% vs. 52.4%, p = 3.7 × 10-8, respectively).
The distribution of insulin gene polymorphisms did not show significant differences between patients with AD, HT, or APS-II and HC.

Conclusion
We demonstrate that the allele "C" of the -2221Msp(C/T) and "A" -23HphI(A/T) insulin gene polymorphisms confer susceptibility to T1D but not to isolated AD, HT or as a part of the APS-II.
Zinc protoporphyrin IX, a heme oxygenase-1 inhibitor, demonstrates potent antitumor effects but is unable to potentiate antitumor effects of chemotherapeutics in mice
Authors: Nowis Dominika , Bugajski Marek , Winiarska Magdalena , Bil Jacek , Szokalska Angelika , Salwa Pawel , Issat Tadeusz , Was Halina , Jozkowicz Alicja , Dulak Jozef , Stoklosa Tomasz , Golab Jakub 
Published: July 2008

Abstract

Background
HO-1 participates in the degradation of heme. Its products can exert unique cytoprotective effects. Numerous tumors express high levels of HO-1 indicating that this enzyme might be a potential therapeutic target. In this study we decided to evaluate potential cytostatic/cytotoxic effects of zinc protoporphyrin IX (Zn(II)PPIX), a selective HO-1 inhibitor and to evaluate its antitumor activity in combination with chemotherapeutics.

Methods
Cytostatic/cytotoxic effects of Zn(II)PPIX were evaluated with crystal violet staining and clonogenic assay. Western blotting was used for the evaluation of protein expression. Flow cytometry was used to evaluate the influence of Zn(II)PPIX on the induction of apoptosis and generation of reactive oxygen species. Knock-down of HO-1 expression was achieved with siRNA. Antitumor effects of Zn(II)PPIX alone or in combination with chemotherapeutics were measured in transplantation tumor models.

Results
Zn(II)PPIX induced significant accumulation of reactive oxygen species in tumor cells. This effect was partly reversed by administration of exogenous bilirubin. Moreover, Zn(II)PPIX exerted potent cytostatic/cytotoxic effects against human and murine tumor cell lines. Despite a significant time and dose-dependent decrease in cyclin D expression in Zn(II)PPIX-treated cells no accumulation of tumor cells in G1 phase of the cell cycle was observed. However, incubation of C-26 cells with Zn(II)PPIX increased the percentage of cells in sub-G1 phase of the cells cycle. Flow cytometry studies with propidium iodide and annexin V staining as well as detection of cleaved caspase 3 by Western blotting revealed that Zn(II)PPIX can induce apoptosis of tumor cells. B16F10 melanoma cells overexpressing HO-1 and transplanted into syngeneic mice were resistant to either Zn(II)PPIX or antitumor effects of cisplatin. Zn(II)PPIX was unable to potentiate antitumor effects of 5-fluorouracil, cisplatin or doxorubicin in three different tumor models, but significantly potentiated toxicity of 5-FU and cisplatin.

Conclusion
Inhibition of HO-1 exerts antitumor effects but should not be used to potentiate antitumor effects of cancer chemotherapeutics unless procedures of selective tumor targeting of HO-1 inhibitors are developed.
Factors influencing the growth hormone peak and plasma insulin-like growth factor I in young adults with pituitary stalk interruption syndrome
Authors: Marcu Mariana , Trivin Christine , Souberbielle Jean-Claude , Brauner Raja 
Published: July 2008

Abstract

Background
The diagnostic criteria for growth hormone (GH) deficiency (GHD) in adolescents and young adults are not yet clearly established.
We evaluated the factors influencing the GH peak and plasma insulin-like growth factor (IGF) I in order to determine the cut-off limits for the diagnosis of GHD during the transition period.

Methods
21 patients treated for GHD due to pituitary stalk interruption syndrome at 5.7 ± 4.1 years were reevaluated at 16.0 ± 1.8 years, 0.6 ± 0.6 years after the end of GH treatment. Group 1 had isolated GHD (n = 9) and group 2 had multiple pituitary deficiencies (n = 12), including deficiencies of thyroid stimulating (n = 12), adrenocorticotropin (n = 8) and gonadotropin (n = 9) hormones.

Results
At diagnosis, group 1 had a greater pituitary height (2.8 ± 1.2 vs 1.6 ± 1.1 mm, P = 0.03) and GH peak (3.8 ± 1.9 vs 1.6 ± 1.5 ng/ml, P < 0.02) than did group 2.
At last evaluation, group 1 had greater GH peak (3.9 ± 1.9 vs 0.2 ± 0.4 ng/ml, P = 0.0001) and plasma IGF I (211 ± 88 vs 78 ± 69 ng/ml, P < 0.002) than did group 2. No group 1 and 9 group 2 patients had an undetectable GH peak, while the 3 others had GH peak below 1 ng/ml.
The GH peak decreased between diagnosis and last evaluation only in group 2 (P < 0.008).

Conclusion
The GH peak response to pharmacological stimulation and the plasma IGF I concentration in young adults with GHD of childhood onset depend on the presence of additional pituitary deficiencies, reflecting a more severe defect of the hypothalamic-pituitary axis. The sex steroids cannot increase the IGF I if the GH secretion is zero.
Logical Analysis of Data (LAD) model for the early diagnosis of acute ischemic stroke
Authors: Reddy Anupama , Wang Honghui , Yu Hua , Bonates Tiberius O , Gulabani Vimla , Azok Joseph , Hoehn Gerard , Hammer Peter L , Baird Alison E , Li King C 
Published: July 2008

Abstract

Background
Strokes are a leading cause of morbidity and the first cause of adult disability in the United States. Currently, no biomarkers are being used clinically to diagnose acute ischemic stroke. A diagnostic test using a blood sample from a patient would potentially be beneficial in treating the disease.

Results
A classification approach is described for differentiating between proteomic samples of stroke patients and controls, and a second novel predictive model is developed for predicting the severity of stroke as measured by the National Institutes of Health Stroke Scale (NIHSS). The models were constructed by applying the Logical Analysis of Data (LAD) methodology to the mass peak profiles of 48 stroke patients and 32 controls. The classification model was shown to have an accuracy of 75% when tested on an independent validation set of 35 stroke patients and 25 controls, while the predictive model exhibited superior performance when compared to alternative algorithms. In spite of their high accuracy, both models are extremely simple and were developed using a common set consisting of only 3 peaks.

Conclusion
We have successfully identified 3 biomarkers that can detect ischemic stroke with an accuracy of 75%. The performance of the classification model on the validation set and on cross-validation does not deteriorate significantly when compared to that on the training set, indicating the robustness of the model. As in the case of the LAD classification model, the results of the predictive model validate the function constructed on our support-set for approximating the severity scores of stroke patients. The correlation and root mean absolute error of the LAD predictive model are consistently superior to those of the other algorithms used (Support vector machines, C4.5 decision trees, Logistic regression and Multilayer perceptron).