bluuebunny/crossref_metadata_2025_split huggingface dataset:
The rapid onset of hyperglycaemia in ZDF rats was associated with a widespread alteration of metabolic proteins implicated in glucose metabolism in the heart
Authors: Lajoie Claude , Béliveau Louise , Trudeau François , Lavoie Nathalie , Massicotte Guy , Gagnon Sylvain , Calderone Angelino 
Published: January 2007

The present study tested the hypothesis that the phosphorylation and regulation of metabolic proteins implicated in glucose homeostasis were impaired in the heart of the type 2 diabetic Zucker-diabetic-fatty (ZDF) rat model. The onset of hyperglycaemia in ZDF rats was not uniform, instead it either progressed rapidly (3–4 weeks) or was delayed (6–8 weeks). In both the early and late onset hyperglycaemic ZDF rats, AMPKα Thr172phosphorylation in the heart was significantly decreased. In the early onset hyperglycaemic ZDF rats, PKB Ser473phosphorylation was reduced, whereas Thr308phosphorylation was significantly increased. In the late onset hyperglycaemic ZDF rats, PKB Ser473phosphorylation was unchanged, but Thr308phosphorylation remained elevated. Cardiac GLUT4 protein and mRNA expression were significantly reduced in the early onset hyperglycaemic ZDF rats, whereas increased protein expression was observed in the late onset hyperglycaemic ZDF rats. In conclusion, the present study has demonstrated that following a more rapid onset of hyperglycaemia, the type 2 diabetic heart is more prone to alterations in the signaling proteins implicated in glucose metabolism.
Characterizing Land–Atmosphere Coupling and the Implications for Subsurface Thermodynamics
Authors: Stieglitz Marc , Smerdon Jason E. 
Published: January 2007

Abstract
The objective of this work is to develop a Simple Land-Interface Model (SLIM) that captures the seasonal and interannual behavior of land–atmosphere coupling, as well as the subsequent subsurface temperature evolution. The model employs the one-dimensional thermal diffusion equation driven by a surface flux boundary condition. While the underlying physics is straightforward, the SLIM framework allows a qualitative understanding of the first-order controls that govern the seasonal coupling between the land and atmosphere by implicitly representing the dominant processes at the land surface. The model is used to perform a suite of experiments that demonstrate how changes in surface air temperature and coupling conditions control subsurface temperature evolution. The work presented here suggests that a collective approach employing both complex and simple models, when joined with analyses of observational data, has the potential to increase understanding of land–atmosphere coupling and the subsequent evolution of subsurface temperatures.
The Seasonal Development of an SST Anomaly in the Indian Ocean and Its Relationship to ENSO
Authors: Nagura Motoki , Konda Masanori 
Published: January 2007

Abstract
The seasonal development of the sea surface temperature (SST) anomaly in the Indian Ocean is investigated in relation to El Niño–Southern Oscillation (ENSO), using NOAA optimally interpolated SST and NCEP reanalysis data. The result shows that the onset season of El Niño affects the seasonal development of surface wind anomalies over the equatorial eastern Indian Ocean (EEIO); these surface wind anomalies, in turn, determine whether the SST anomaly in the EEIO evolves into the eastern pole of the dipole pattern. In years when the dipole pattern develops, surface zonal wind anomalies over the EEIO switch from westerly to easterly in spring as La Niña switches to El Niño. The seasonal zonal wind over the EEIO also switches from westerly to easterly in spring, and the anomalous wind strengthens seasonal wind from winter to summer. Stronger winds and resultant thermal forcings produce the negative SST anomaly in the EEIO in winter, and its amplitude increases in summer. The SST anomaly becomes the eastern pole of the dipole pattern in fall. In contrast, if the change from La Niña to El Niño is delayed until late summer/fall or if La Niña persists throughout the year, a westerly anomaly persists from winter to summer over the EEIO. The persistent westerly anomaly strengthens the wintertime climatological westerlies and weakens the summertime easterlies. Therefore, negative SST anomalies are produced in the EEIO in winter, but the amplitude decreases in summer, and the eastern pole is not present in fall. The above explanation also applies to onset years of La Niña if the signs of the anomalies are reversed.
Global Vegetation and Climate Change due to Future Increases in CO2 as Projected by a Fully Coupled Model with Dynamic Vegetation*
Authors: Notaro Michael , Vavrus Steve , Liu Zhengyu 
Published: January 2007

Abstract
Transient simulations are presented of future climate and vegetation associated with continued rising levels of CO2. The model is a fully coupled atmosphere–ocean–land–ice model with dynamic vegetation. The impacts of the radiative and physiological forcing of CO2 are diagnosed, along with the role of vegetation feedbacks. While the radiative effect of rising CO2 produces most of the warming, the physiological effect contributes additional warming by weakening the hydrologic cycle through reduced evapotranspiration. Both effects cause drying over tropical rain forests, while the radiative effect enhances Arctic and Indonesian precipitation.
A global greening trend is simulated primarily due to the physiological effect, with an increase in photosynthesis and total tree cover associated with enhanced water-use efficiency. In particular, tree cover is enhanced by the physiological effect over moisture-limited regions. Over Amazonia, South Africa, and Australia, the radiative forcing produces soil drying and reduced forest cover. A poleward shift of the boreal forest is simulated as both the radiative and physiological effects enhance vegetation growth in the northern tundra and the radiative effect induces drying and summertime heat stress on the central and southern boreal forest. Vegetation feedbacks substantially impact local temperature trends through changes in albedo and evapotranspiration. The physiological effect increases net biomass across most land areas, while the radiative effect results in an increase over the tundra and decrease over tropical forests and portions of the boreal forest.
Convective Precipitation Variability as a Tool for General Circulation Model Analysis
Authors: DeMott Charlotte A. , Randall David A. , Khairoutdinov Marat 
Published: January 2007

AbstractPrecipitation variability is analyzed in two versions of the Community Atmospheric Model (CAM), the standard model, CAM, and a “multiscale modeling framework” (MMF), in which the cumulus parameterization has been replaced with a cloud-resolving model. Probability distribution functions (PDFs) of daily mean rainfall in three geographic locations [the Amazon Basin and western Pacific in December–February (DJF) and the North American Great Plains in June–August (JJA)] indicate that the CAM produces too much light–moderate rainfall (10 ∼ 20 mm day−1), and not enough heavy rainfall, compared to observations. The MMF underestimates rain contributions from the lightest rainfall rates but correctly simulates more intense rainfall events. These differences are not always apparent in seasonal mean rainfall totals.Analysis of 3–6-hourly rainfall and sounding data in the same locations reveals that the CAM produces moderately intense rainfall as soon as the boundary layer energizes. Precipitation is also concurrent with tropospheric relative humidity and lifted parcel buoyancy increases. In contrast, the MMF and observations are characterized by a lag of several hours between boundary layer energy buildup and precipitation, and a gradual increase in the depth of low-level relative humidity maximum prior to rainfall.The environmental entrainment rate selection in the CAM cumulus parameterization influences CAM precipitation timing and intensity, and may contribute to the midlevel dry bias in that model. The resulting low-intensity rainfall in the CAM leads to rainfall–canopy vegetation interactions that are different from those simulated by the MMF. The authors present evidence suggesting that this interaction may artificially inflate North American Great Plains summertime rainfall totals in the CAM.
Genetics of parentally reported asthma, eczema and rhinitis in 5-yr-old twins
Authors: van Beijsterveldt C. E. M. , Boomsma D. I. 
Published: January 2007

The aim of the present study was to examine the genetic and environmental contributions to the individual differences in susceptibility to asthma, eczema and rhinitis in childhood and their role in the association among these conditions.Information on asthma, eczema and rhinitis was obtained by parental report. Parents were asked whether a physician had ever diagnosed the condition. Complete data were available for 8,633 5-yr-old twin pairs born between 1986 and 1998.The frequency of parentally reported asthma, eczema and rhinitis was 8.7, 16.8 and 4.4%, respectively, and was higher in males than females. Genetic factors accounted for ∼90% of the variance in the susceptibility to asthma, eczema and rhinitis. The magnitude of genetic factors did not differ between males and females. The remaining part of the variance was explained by environmental factors not shared by family members. The phenotypic correlations between parentally reported asthma, eczema and rhinitis were moderate and mainly mediated by the same genetic factors.The high heritability and the limited influence of shared environmental factors may point to gene x environment interactions. Future research should focus on this type of interaction processes.
Release of the antimicrobial peptide LL-37 from DNA/F-actin bundles in cystic fibrosis sputum
Authors: Bucki R. , Byfield F. J. , Janmey P. A. 
Published: January 2007

Cationic antibacterial peptides (ABPs) are secreted in the airways and function in the first line of defence against infectious agents. They attack multiple molecular targets to cooperatively penetrate and disrupt microbial surfaces and membrane barriers. Antibacterial properties of ABPs, including cathelicidin LL-37, are reduced in cystic fibrosis (CF) airways as a result of direct interaction with DNA and filamentous (F)-actin.Microscopic evaluation of a mixed solution of DNA and F-actin, after the addition of rhodamine-B-labelled LL-37 peptide, revealed the presence of a bundle structure similar to that present in CF sputum. Analysis of CF sputum after centrifugation showed that LL-37 was mostly bound to components of the pellet fraction containing DNA, F-actin and cell remnants. Factors that dissolve DNA/actin bundles and fluidise CF sputum, such as Dornase alfa (recombinant human DNase I), gelsolin, polyaspartate or their combinations, increased the amount of LL-37 peptide detected in the supernatant of CF sputum.The presence of the bacterial endotoxin lipopolysaccharide (LPS) in CF sputum and the ability of LPS to inhibit the antibacterial activity of LL-37 suggests that inactivation of LL-37 function in CF sputum partially results from its interaction with LPS. LL-37–LPS interaction was prevented by an LPS-binding protein (LBP)-derived peptide known for its ability to neutralise LPS, whereas LBPW91A, a mutant peptide that lacks ability to bind LPS, had no effect.A combination of factors that dissolve DNA/filamentous-actin aggregates together with lipopolysaccharide-binding agents may represent a potential treatment for the chronic infections that occur in cystic fibrosis airways.
Socioeconomic status, asthma and chronic bronchitis in a large community-based study
Authors: Ellison-Loschmann L. , Sunyer J. , Plana E. , Pearce N. , Zock J-P. , Jarvis D. , Janson C. , Antó J. M. , Kogevinas M. 
Published: January 2007

The present study investigated the relationship between socioeconomic status, using measures of occupational class and education level, and the prevalence and incidence of asthma (with and without atopy) and chronic bronchitis using data from the European Community Respiratory Health Survey (ECRHS).Asthma and chronic bronchitis were studied prospectively within the ECRHS (n = 9,023). Incidence analyses comprised subjects with no history of asthma or bronchitis at baseline. Asthma symptoms were also assessed as a continuous score.Bronchitis risk was associated with low educational level (prevalence odds ratio (POR) 1.9; 95% confidence interval (CI) 1.4–2.8) and occupational class (1.8; 1.2–2.7). Incident bronchitis also increased with low educational level (risk ratio (RR) 2.8; 95%CI 1.5–5.4). Prevalent and incident asthma with no atopy were associated with low educational level. Subjects in the low occupational class (incident risk ratio (IRR) 1.4; 95%CI 1.2–1.7) and education group (IRR 1.3; 95% CI 1.1–1.6) had higher mean asthma scores than those in higher socioeconomic groups.Lower educational level was associated with increased risk of prevalent and incident chronic bronchitis and asthma with no atopy. Lower socioeconomic groups tended to have a higher prevalence and incidence of asthma, particularly higher mean asthma scores. Adjustment for variables associated with asthma and bronchitis explained little of the observed health differences by socioeconomic status.
Effect of tuberculin skin testing on aMycobacterium tuberculosis-specific interferon-γ assay
Authors: Leyten E. M. S. , Prins C. , Bossink A. W. J. , Thijsen S. , Ottenhoff T. H. M. , van Dissel J. T. , Arend S. M. 
Published: January 2007

Recently, interferon-γ release assays (IGRA) for specific diagnosis ofMycobacterium tuberculosisinfection have become available. In recent UK tuberculosis (TB) guidelines, it has been advised to screen for latentM. tuberculosisinfection using the tuberculin skin test (TST), followed by IGRA if the TST is positive. Since TST can boost immune responses to tuberculin, the present authors evaluated whether TST administration affects the result of QuantiFERON®-TB Gold in-tube (QFT-GIT), a whole blood-based IGRA.QFT-GIT was performed on the day of TST administration and the day of reading in 15 TST-negative subjects, 46 TST-positive subjects with recent or remote exposure toM. tuberculosisand five cured TB patients.No systematic boosting of QFT-GIT responses from negative to positive was observed. Only in a few TST-positive persons did TST enhance pre-existing QFT-GIT responses.Screening for latentMycobacterium tuberculosisinfection using tuberculin skin testing followed by interferon-γ release assays on the day of reading is a reliable approach, as the specificity of QuantiFERON®-TB Gold in-tube is not affected by prior tuberculin skin test administration.
A prediction rule for elderly primary-care patients with lower respiratory tract infections
Authors: Bont J. , Hak E. , Hoes A. W. , Schipper M. , Schellevis F. G. , Verheij T. J. M. 
Published: January 2007

Prognostic scores for lower respiratory tract infections (LRTI) have been mainly derived in a hospital setting. The current authors have developed and validated a prediction rule for the prognosis of acute LRTI in elderly primary-care patients.Data including demographics, medication use, healthcare use and comorbid conditions from 3,166 episodes of patients aged ≥65 yrs visiting the general practitioner (GP) with LRTI were collected. Multiple logistic regression analysis was used to construct a predictive model. The main outcome measure was 30-day hospitalisation or death. The Second Dutch Survey of GPs was used for validation.The following were independent predictors of 30-day hospitalisation or death: increasing age; previous hospitalisation; heart failure; diabetes; use of oral glucocorticoids; previous use of antibiotics; a diagnosis of pneumonia; and exacerbation of chronic obstructive pulmonary disease. A prediction rule based on these variables showed that the outcome increased directly with increasing scores: 3, 10 and 31% for scores of <2 points, 3–6 and ≥7 points, respectively. Corresponding figures for the validation cohort were 3, 11 and 26%, respectively.This simple prediction rule can help the primary-care physician to differentiate between high- and low-risk patients. As a possible consequence, low-risk patients may be suitable for home treatment, whereas high-risk patients might be monitored more closely in a homecare or hospital setting. Future studies should assess whether information on signs and symptoms can further improve this prediction rule.
Specification of primordial germ cells in medaka (Oryzias latipes)
Authors: Herpin Amaury , Rohr Stefan , Riedel Dietmar , Kluever Nils , Raz Erez , Schartl Manfred 
Published: January 2007

AbstractBackgroundPrimordial germ cells (PGCs) give rise to gametes that are responsible for the development of a new organism in the next generation. Two modes of germ line specification have been described: the inheritance of asymmetrically-localized maternally provided cytoplasmic determinants and the induction of the PGC fate by other cell types.PGCs specification in zebrafish appears to depend on inheritance of germ plasm in which several RNA molecules such asvasaandnanosreside. Whether the specification mode of PGCs found in zebrafish is general for other fish species was brought into question upon analysis ofolvasexpression – thevasahomologue in another teleost, medaka (Oryzias latipes). Here, in contrast to the findings in zebrafish, the PGCs are found in a predictable position relative to a somatic structure, the embryonic shield. This finding, coupled with the fact thatvasamRNA, which is localized to the germ plasm of zebrafish but does not label a similar structure in medaka opened the possibility of fundamentally different mechanisms governing PGC specification in these two fish species.ResultsIn this study we addressed the question concerning the mode of PGC specification in medaka using embryological experiments, analysis of RNA stability in the PGCs and electron microscopy observations. Dramatic alterations in the somatic environment, i.e. induction of a secondary axis or mesoderm formation alteration, did not affect the PGC number. Furthermore, the PGCs of medaka are capable of protecting specific RNA molecules from degradation and could therefore exhibit a specific mRNA expression pattern controlled by posttrancriptional mechanisms. Subsequent analysis of 4-cell stage medaka embryos using electron microscopy revealed germ plasm-like structures located at a region corresponding to that of zebrafish germ plasm.ConclusionTaken together, these results are consistent with the idea that in medaka the inheritance of maternally provided asymmetrically-localized cytoplasmic determinants directs cells to assume the germ line fate similar to zebrafish PGCs.
A single institution experience of combined modality management of extra skeletal Ewings sarcoma
Authors: Venkitaraman Ramachandran , George Mathew K , Ramanan S Ganapathy , Sagar TG 
Published: January 2007

Abstract

Background
Extraskeletal Ewings sarcoma are rare tumors for which there is no consensus on optimal management.

Methods
A retrospective review of the clinical features, treatment and outcome of patients with extraskeletal Ewings sarcoma who reported to a single institution between January 1992 – December 2003 is reported.

Results
A total of 19 patients with extraskeletal Ewings sarcoma were identified. Of these, 4 patients had metastatic disease at presentation and 15 patients with non-metastatic disease received combined modality treatment with primary combination chemotherapy followed by local treatment with radiotherapy or surgery. Disease free survival and overall survival for patients with non metastatic disease after combined modality treatment were 60% and 30% respectively. The significant predictors for prolonged disease free survival and overall survival were high haemoglobin(p = 0.002), low lactate dehydrogenase (p = 0.028), chemotherapy with Vincristine, Adriamycin, Cyclophosphamide, Ifosfamide and Etoposide regime (p = 0.008) and complete response to chemotherapy (p = 0.001).

Conclusion
Aggressive combination chemotherapy followed by complete surgery or radiotherapy to a dose of more than 50 Gy is essential to confer optimal outcome for patients with extraskeletal ewings sarcoma.
The role of PDGF in radiation oncology
Authors: Li Minglun , Jendrossek Verena , Belka Claus 
Published: January 2007

AbstractPlatelet-derived growth factor (PDGF) was originally identified as a constituent of blood serum and subsequently purified from human platelets. PDGF ligand is a dimeric molecule consisting of two disulfide-bonded chains from A-, B-, C- and D-polypeptide chains, which combine to homo- and heterodimers. The PDGF isoforms exert their cellular effects by binding to and activating two structurally related protein tyrosine kinase receptors. PDGF is a potent mitogen and chemoattractant for mesenchymal cells and also a chemoattractant for neutrophils and monocytes. In radiation oncology, PDGF are important for several pathologic processes, including oncogenesis, angiogenesis and fibrogenesis. Autocrine activation of PDGF was observed and interpreted as an important mechanism involved in brain and other tumors. PDGF has been shown to be fundamental for the stability of normal blood vessel formation, and may be essential for the angiogenesis in tumor tissue. PDGF also plays an important role in the proliferative disease, such as atherosclerosis and radiation-induced fibrosis, regarding its proliferative stimulation of fibroblast cells. Moreover, PDGF was also shown to stimulate production of extracellular matrix proteins, which are mainly responsible for the irreversibility of these diseases. This review introduces the structural and functional properties of PDGF and PDGF receptors and discusses the role and mechanism of PDGF signaling in normal and tumor tissues under different conditions in radiation oncology.
Qualitative network models and genome-wide expression data define carbon/nitrogen-responsive molecular machines in Arabidopsis
Authors: Gutiérrez Rodrigo A , Lejay Laurence V , Dean Alexis , Chiaromonte Francesca , Shasha Dennis E , Coruzzi Gloria M 
Published: January 2007

Abstract
Background
Carbon (C) and nitrogen (N) metabolites can regulate gene expression in Arabidopsis thaliana. Here, we use multinetwork analysis of microarray data to identify molecular networks regulated by C and N in the Arabidopsis root system.

Results
We used the Arabidopsis whole genome Affymetrix gene chip to explore global gene expression responses in plants exposed transiently to a matrix of C and N treatments. We used ANOVA analysis to define quantitative models of regulation for all detected genes. Our results suggest that about half of the Arabidopsis transcriptome is regulated by C, N or CN interactions. We found ample evidence for interactions between C and N that include genes involved in metabolic pathways, protein degradation and auxin signaling. To provide a global, yet detailed, view of how the cell molecular network is adjusted in response to the CN treatments, we constructed a qualitative multinetwork model of the Arabidopsis metabolic and regulatory molecular network, including 6,176 genes, 1,459 metabolites and 230,900 interactions among them. We integrated the quantitative models of CN gene regulation with the wiring diagram in the multinetwork, and identified specific interacting genes in biological modules that respond to C, N or CN treatments.

Conclusion
Our results indicate that CN regulation occurs at multiple levels, including potential post-transcriptional control by microRNAs. The network analysis of our systematic dataset of CN treatments indicates that CN sensing is a mechanism that coordinates the global and coordinated regulation of specific sets of molecular machines in the plant cell.