bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel protein C6ORF120 promotes liver fibrosis by activating hepatic stellate cells through the PI3K/Akt/mTOR pathway
Authors: Wang Xin , Liu Hui , Wang Yuqi , Wang Peng , Yi Yunyun , Lin Yingying , Li Xin 
Published: March 2024

AbstractBackground and AimThe role of C6ORF120 in promoting CCL4‐induced hepatic fibrosis and its possible mechanisms were explored in C6orf120 knockout rats (C6orf120−/−) and LX‐2 cells (a type of human hepatic stellate cell line).MethodsIn vivo experiments, wild‐type and C6orf120−/− rats were used to investigate the function of C6ORF120. In the in vitro experiments, C6ORF120 recombinant protein (rC6ORF120) at a concentration of 200 ng/mL was used to stimulate LX‐2 cells. Sirius Red staining, Masson staining, western blotting, polymerase chain reaction, immunohistochemistry, and immunofluorescence were used to explore fibrosis‐associated factors.ResultsC6orf120−/− rats showed mild fibrosis and liver injury in the CCL4‐induced liver fibrosis model. Furthermore, RNA‐seq revealed that C6orf120−/− rats had less extracellular matrix deposition and activated stellate cells. Consistent with the in vivo, the rC6ORF120 induced LX‐2 cell activation. Moreover, mechanistic studies revealed that the p‐PI3K/PI3K, p‐Akt/Akt, and p‐mTOR/mTOR levels were significantly elevated and LY294002 (a PI3K/Akt/mTOR typical pathway inhibitor) reversed the function of C6ORF120 in activating LX‐2 cells.ConclusionC6ORF120 could activate hepatic stellate cells and promote hepatic fibrosis via the PI3K/Akt/mTOR signaling pathway.
Interviews with policymakers in Australian health policy: Understanding the process of policy development
Authors: Ingram Kelsey , Aley Melanie J. , Irving Michelle , Wallace Janet 
Published: March 2024

AbstractObjectiveTo determine the barriers and enablers oral health and chronic disease policymakers identify during policy development.MethodsSemi‐structured interview questions were developed utilizing an interview guide and applying a knowledge mobilization framework. Reflective thematic analysis of the data was completed based on the research of Braun and Clarke encompassing a critical realist approach.ResultsTwelve policymakers were interviewed. Policymakers reported barriers to accessing evidence including the sheer volume of information and a lack of: research summaries, comprehensive oral health data sets, open access articles, consistency of reporting, and time. They did find relationships with experts and intermediaries crucial at times to policy development. Co‐creation of research was also a key enabler for policymakers.ConclusionsThis study highlights that policymakers find evidence crucial during the development of policy and often use it to advocate for policies. However, the links between poor oral health; and cardiovascular disease, diabetes, and cognitive impairment are not well recognized among chronic disease policymakers. It is important that oral health researchers and policymakers work to close this gap. The treatment of poor oral health is a global population health issue. It is imperative that evidence of these links is implemented into health policy for the treatment and prevention of chronic diseases and improved quality of life for individuals living with chronic diseases.
Hormone replacement therapy and periodontitis progression in postmenopausal women: A prospective cohort study
Authors: Man Ying , Zhang Cui , Cheng Cheng , Yan Laiqi , Zong Min , Niu Feifei 
Published: March 2024

AbstractObjectiveThis study aimed to investigate the responses of periodontal environment to hormone replacement therapy (HRT) in postmenopausal women with or without periodontitis.BackgroundHRT is a common and effective strategy for controlling menopausal symptoms, while the changes of periodontal environment under it, particularly in postmenopausal women with periodontitis, remain unclear.MethodsAs a prospective cohort study, a total of 97 postmenopausal women receiving HRT were screened, including 47 with and 50 without periodontitis. Correspondingly, 97 women did not receiving HRT were screened as controls during the same period. The full‐mouth sulcus bleeding index (SBI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL) were measured using periodontal probes. The levels of interleukin‐6 (IL‐6) and tumor necrosis factor α (TNF‐α) in the gingival crevicular fluid were measured using enzyme‐linked immunosorbent assay. In addition, cone beam computed tomography was performed to measure the alveolar bone height (ABH) and bone mineral density (BMD).ResultsIn postmenopausal women without periodontitis, no significantly changes on periodontal parameters were observed after HRT. In women with stage II periodontitis, SBI, BOP, IL‐6, and TNF‐α were significant decreased after one year and two years of HRT. Compared to the controls, women with stage II periodontitis who underwent HRT had significantly lower CAL and ABH and higher BMD in the second year. The incidence of at least one site with CAL increase ≥1 mm between baseline and 2 years was significantly lower in the HRT group than in the control group in women with stage II periodontitis. In addition, HRT was significantly associated with a decrease in SBI, BOP, IL‐6, and TNF‐α in the first year and with a decrease in CAL, SBI, BOP, IL‐6, and ABH and an increase in BMD in the second year.ConclusionsIn postmenopausal women with stage II periodontitis, HRT is associated with the alleviation of inflammation within two years and the remission of alveolar bone loss in the second year. HRT appears to decrease the incidence of CAL increase ≥1 mm within 2 years in women with periodontitis by inhibiting inflammation and alveolar bone loss.
Bridging the innovation gap: Why organizational climate matters for leveraging innovation from supply networks
Authors: Son Byung‐Gak , Ries Jörg M. , Subramanian Nachiappan , Kim Seongtae 
Published: March 2024

AbstractRecent studies have provided empirical evidence that innovation performance is related to the way a firm is embedded in its supply network, specifically the centrality of its network position, but it remains unclear why some firms can use inputs from suppliers better than others, despite having comparable structural characteristics in their supply networks. Drawing on theories of social networks and organizational climate, this study examines the role of buying firms' organizational climate for innovation. It uses several structured and unstructured datasets for S&P 500 firms and applies count regression models to test hypotheses. Supply network data from FactSet were analyzed to determine the degree centrality of a buying firm. Computer‐aided content analysis was used to capture the organizational climate of buying firms based on online employee reviews collected from Glassdoor. The results suggest a positive relationship between the degree centrality and the innovation performance of buying firms. Moreover, certain facets of the organizational climate related to learning, including rewards and career progress, as well as work pressure management, affect the link between the degree centrality of a buying firm and its innovation performance. In conclusion, this study enhances the understanding of the connection between supply networks and innovation. It highlights the crucial role of a firm‐level factor, specifically the influential facets of organizational climate for learning, in determining innovation performance.
Labor market monopsony power in the manufacturing sector of four Sub‐Saharan African countries
Authors: Haque Samiul , Delgado Micheal S. 
Published: March 2024

AbstractWe estimate labor market monopsony power among manufacturing firms in four Sub‐Saharan African countries using a parametric production function approach on panel dataset. Pooled estimate suggests that wages are approximately 38 percent of the marginal revenue product of labor, implying a labor supply elasticity of 0.62. Nonparametric robustness checks indicate these results are robust to concerns over parametric model misspecification. Departure from competitive labor market leads to approximately 50.80 percent higher employer rent, 75.61 percent lower employee rent, and 15.95 percent deadweight loss. Overall, our results are suggestive of monopsonistic labor markets.
The transcription factor bZIP44 cooperates with MYB10 and MYB72 to regulate the response of Arabidopsis thaliana to iron deficiency stress
Authors: Wu Xi , Jia Yafeng , Ma Qian , Wang Tingting , Xu Jiena , Chen Hongli , Wang Mingxia , Song Hui , Cao Shuqing 
Published: March 2024

Summary
Nicotianamine (NA) plays a crucial role in transporting metal ions, including iron (Fe), in plants; therefore, NICOTIANAMINE SYNTHASE (NAS) genes, which control NA synthesis, are tightly regulated at the transcriptional level. However, the transcriptional regulatory mechanisms of NAS genes require further investigations.
In this study, we determined the role of bZIP44 in mediating plant response to Fe deficiency stress by conducting transformation experiments and assays.
bZIP44 positively regulated the response of Arabidopsis to Fe deficiency stress by interacting with MYB10 and MYB72 to enhance their abilities to bind at NAS2 and NAS4 promoters, thereby increasing NAS2 and NAS4 transcriptional levels and promote NA synthesis.
In summary, the transcription activities of bZIP44, MYB10, and MYB72 were induced in response to Fe deficiency stress, which enhanced the interaction between bZIP44 and MYB10 or MYB72 proteins, synergistically activated the transcriptional activity of NAS2 and NAS4, promoted NA synthesis, and improved Fe transport, thereby enhancing plant tolerance to Fe deficiency stress.
Mucoadhesive delivery systems for oral candidiasis treatment: A systematic review and meta‐analysis
Authors: Sugio Carolina Yoshi Campos , Garcia Amanda Aparecida Maia Neves , Kitamoto Klaryssa Akemi de Araujo , Santiago Júnior Joel Ferreira , Soares Simone , Porto Vinicius Carvalho , Urban Vanessa Migliorini , Ferrari Priscileila Colerato , Fernandes Maria Helena , Neppelenbroek Karin Hermana 
Published: March 2024

AbstractObjectivesThis systematic review and meta‐analysis aimed to evaluate the clinical and mycological effectiveness of mucoadhesives as vehicles for drugs or natural products in the treatment of oral candidiasis.Materials and methodsThe search for articles was carried out in the Medline/PubMed, SCOPUS, EMBASE, Web of Science, Cochrane Library, and SciELO databases before August 2023. We selected the studies, extracted the data, evaluated the study quality, graded the evidence, performed the risk of bias, and carried out meta‐analysis.ResultsA total of 389 potentially relevant articles were identified, and 11 studies (1869 participants) met the inclusion criteria of the systematic review. The overall risk of bias was considered low. The most common presentation of mucoadhesives was tablets, with miconazole being the most frequently drug used in the delivery system. Mucoadhesives demonstrated comparable efficacy with topical or systemic antifungal agents, with no significant differences between treatments in terms of clinical (RR = 0.907; 95CI = 0.3–1.297; p = 0.591; I2 = 64.648) or mycological (RR = 0.95; 95CI = 0.667–1.360; p = 0.789; I2 = 73.271) efficacy.ConclusionsMucoadhesives may be a suitable alternative to conventional treatments, with the advantage of reducing the frequency of application by up to 5 times and the daily dosage by up to 20 times.
Halotolerant Pseudomonas koreensisS4T10 mitigate salt and drought stress in Arabidopsis thaliana
Authors: Kalleku Justine Nathanael , Ihsan Samsoor , Al‐Azzawi Tiba Nazar Ibrahim , Khan Murtaza , Hussain Adil , Chebitok Felistus , Das Ashim Kumar , Moon Yong‐Sun , Mun Bong‐Gyu , Lee In‐Jung , Ali Sajid , Yun Byung‐Wook 
Published: March 2024

AbstractSalt and drought are documented among the most detrimental and persistent abiotic stresses for crop production. Here, we investigated the impact of Pseudomonas koreensis strain S4T10 on plant performance under salt and drought stress. Arabidopsis thaliana Col‐0 wild type and atnced3 mutant plants were inoculated with P. koreensis or tap water and exposed to NaCl (100 mM) for five days and drought stress by withholding water for seven days. P. koreensis significantly enhanced plant biomass and photosynthetic pigments under salt and drought stress conditions. Moreover, P. koreensis activated the antioxidant defence by modulating glutathione (GSH), superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) activities to scavenge the reactive oxygen species produced due to the stress. In addition, the application of P. koreensis upregulated the expression of genes associated with antioxidant responses, such as AtCAT1, AtCAT3, and AtSOD. Similarly, genes linked to salt stress, such as AtSOS1, AtSOS2, AtSOS3, AtNHX1, and AtHKT1, were also upregulated, affirming the positive role of P. koreensis S4T10 in streamlining the cellular influx and efflux transport systems during salt stress. Likewise, the PGPB inoculation was observed to regulate the expression of drought‐responsive genes AtDREB2A, AtDREB2B, and ABA‐responsive genes AtAO3, AtABA3 indicating that S4T10 enhanced drought tolerance via modulation of the ABA pathway. The results of this study affirm that P. koreensis S4T10 could be further developed as a biofertilizer to mitigate salt and drought stress at the same time.
What can nurses learn from patient's needs and wishes when developing an evidence‐based quality improvement learning culture? A qualitative study
Authors: Giesen Jeltje , Timmerman Ilse , Bakker‐Jacobs Annick , Berings Marjolein , Huisman‐de Waal Getty , Van Vught Anneke , Vermeulen Hester 
Published: March 2024

AbstractBackgroundPatient participation is fundamental in nursing care and has yielded benefits for patient outcomes. However, despite their compassionate care approach, nurses do not always incorporate patients' needs and wish into evidence‐based practice, quality improvement or learning activities. Therefore, a shift to continuous quality improvement based on evidence‐based practice is necessary to enhance the quality of care. The patient's opinion is an essential part of this process. To establish a more sustainable learning culture for evidence‐based quality improvement, it is crucial that nurses learn alongside their patients. However, to promote this, nurses require a deeper understanding of patients' care preferences.ObjectiveTo explore patients' needs and wishes towards being involved in care processes that nurses can use in developing an evidence‐based quality improvement learning culture.MethodsA qualitative study was conducted in two hospital departments and one community care team. In total, 18 patients were purposefully selected for individual semi‐structured interviews with an average of 15 min. A framework analysis based on the fundamental of care framework was utilised to analyse the data deductively. In addition, inductive codes were added to patients' experiences beyond the framework. For reporting this study, the SRQR guideline was used.ResultsParticipants needed a compassionate nurse who established and sustained a trusting relationship. They wanted nurses to be present and actively involved during the care delivery. Shared decision‐making improved when nurses offered fair, clear and tailored information. Mistrust or a disrupted nurse–patient relationship was found to be time‐consuming and challenging to restore.ConclusionsResults confirmed the importance of a durable nurse–patient relationship and showed the consequences of nurses' communication on shared decision‐making. Insights into patients' care preferences are essential to stimulate the development of an evidence‐based quality improvement learning culture within nursing teams and for successful implementation processes.
Long‐ and short‐term frequent attenders' perceptions of patient‐centredness in Finnish primary healthcare: A cross‐sectional survey and the equivalence of the factor structure of the 36‐item patient‐centred primary care instrument
Authors: Moona Huhtakangas , Mimmi Tolvanen , Helvi Kyngäs , Outi Kanste 
Published: March 2024

AbstractAimThe study aimed to compare long‐ and short‐term frequent attenders' (FAs) perceptions of patient‐centredness and to assess the equivalence of the factor structure of patient‐centred primary care (PCPC) instrument for long‐ and short‐term FAs.MethodsA cross‐sectional survey of long‐term (n = 234) and short‐term (n = 261) FAs within the primary healthcare of one city in Finland. The data collected in January–July 2020 via ‘the 36‐item PCPC instrument’ were analysed using Pearson's chi‐squared test, Mann–Whitney U‐test, Cronbach's alpha, and multigroup confirmatory factor analysis (MGCFA).ResultsLong‐term FAs had worse perceptions of patient‐centredness than short‐term FAs across all subscales: patients’ preferences, physical comfort, coordination of care, continuity and transition, emotional support, access to care, information and education, and family and friends. The MGCFA indicated a good fit for the predicted eight‐factor model.ConclusionsLong‐term FAs’ care needs are currently not being met, indicating the need for considering how the way healthcare services are arranged and care is provided affects prolonged and high care needs. Various dimensions of patient‐centredness need to be addressed when developing not only FAs’ care but also wider service paths. A distinction should be made between long‐ and short‐term frequent attendance when identifying and addressing FAs’ service needs.
Presentation, management, and outcomes of norovirus in adult and pediatric solid organ and hematopoietic stem cell transplant recipients: A multicenter, retrospective study
Authors: Callegari Michelle , Danziger‐Isakov Lara A. , Rose Anne , Kaul Daniel , Shaffer Kelly , Chong Pearlie P. , Florescu Diana , German Kaci , Avery Robin , Nguyen M. Hong , Wildfeuer Brett , Michaels Marian G. , Green Michael , Guo Kexin , Zhao Lihui , Daud Amna , Ison Michael G. 
Published: March 2024

AbstractBackgroundNorovirus (NoV) can cause chronic relapsing and remitting diarrhea in immunocompromised patients.  Few multicenter studies have described the clinical course, outcomes, and complications of chronic NoV in transplant recipients.MethodsA multicenter retrospective study of adult and pediatric SOT and HSCT recipients diagnosed with NoV between November 1, 2017, and February 28, 2021. Data were obtained from electronic medical records (EMR) and entered into a central REDCap database. Descriptive statistics were calculated.ResultsA total of 280 NoV+ patients were identified across eight sites. The majority were adults (74.1%) and SOT recipients (91.4%). Initial diagnosis of NoV occurred a median of 36 months post‐Tx (IQR [15.0, 90.0]). Most NoV cases had >3 diarrheal episodes daily (66.0%), nausea and vomiting (60.1%). Duration of diarrhea varied greatly (median = 10 days, mean = 85.9 days, range (1, 2100)). 71.3% were hospitalized. Adjustment of immunosuppression, including reduction and discontinuation of mToR inhibitor, CNI, and/or MMF, was the most common management intervention for NoV. Other therapies resulted only in temporary improvement. Four patients died within 30 days and three others died by 180 days postdiagnosis. Clinically significant renal dysfunction was observed in 12.5% by 30 days and 21.4% by 180 days post‐NoV diagnosis.ConclusionIn HSCT and SOT patients, NoV frequently resulted in severe symptoms, prolonged diarrhea (30% persistent with diarrhea for >30 days), and clinically significant renal dysfunction (up to 21% of patients). Utilized therapies did not reliably result in the resolution of infection demonstrating the need for more effective treatment.
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First investigation into the genetic control of meiosis in sugarcane
Authors: Reis Soares Nina , Costa Zirlane Portugal , Marques João Paulo Rodrigues , Garsmeur Olivier , Sampaio Carneiro Monalisa , Monteiro Vitorello Cláudia Barros , D'Hont Angélique , Vieira Maria Lucia Carneiro 
Published: March 2024

SUMMARYThe sugarcane (Saccharum spp.) genome is one of the most complex of all. Modern varieties are highly polyploid and aneuploid as a result of hybridization between Saccharum officinarum and S. spontaneum. Little research has been done on meiotic control in polyploid species, with the exception of the wheat Ph1 locus harboring the ZIP4 gene (TaZIP4‐B2) which promotes pairing between homologous chromosomes while suppressing crossover between homeologs. In sugarcane, despite its interspecific origin, bivalent association is favored, and multivalents, if any, are resolved at the end of prophase I. Thus, our aim herein was to investigate the purported genetic control of meiosis in the parental species and in sugarcane itself. We investigated the ZIP4 gene and immunolocalized meiotic proteins, namely synaptonemal complex proteins Zyp1 and Asy1. The sugarcane ZIP4 gene is located on chromosome 2 and expressed more abundantly in flowers, a similar profile to that found for TaZIP4‐B2. ZIP4 expression is higher in S. spontaneum a neoautopolyploid, with lower expression in S. officinarum, a stable octoploid species. The sugarcane Zip4 protein contains a TPR domain, essential for scaffolding. Its 3D structure was also predicted, and it was found to be very similar to that of TaZIP4‐B2, reflecting their functional relatedness. Immunolocalization of the Asy1 and Zyp1 proteins revealed that S. officinarum completes synapsis. However, in S. spontaneum and SP80‐3280 (a modern variety), no nuclei with complete synapsis were observed. Importantly, our results have implications for sugarcane cytogenetics, genetic mapping, and genomics.
Prediction of the antigenic regions in eight RhD variants identified by computational biology
Authors: Trueba‐Gómez Rocio , Rosenfeld‐Mann Fany , Estrada‐Juárez Higinio 
Published: March 2024

AbstractBackground and ObjectivesChanges in RHD generate variations in protein structure that lead to antigenic variants. The classical model divides them into quantitative (weak and Del) and qualitative (partial D). There are two types of protein antigens: linear and conformational. Computational biology analyses the theoretical assembly of tertiary protein structures and allows us to identify the ‘topological’ differences between isoforms. Our aim was to determine the theoretical antigenic differences between weak RhD variants compared with normal RhD based on structural analysis using bioinformatic techniques.Materials and MethodsWe analysed the variations in secondary structures and hydrophobicity of RHD*01, RHD*01W.1, W2, W3, RHD*09.03.01, RHD*09.04, RHD*11, RHD*15 and RHD*21. We then modelled the tertiary structure and calculated their probable antigenic regions, intra‐protein interactions, displacement and membrane width and compared them with Rhce.ResultsThe 10 proteins are similar in their secondary structure and hydrophobicity, with the main differences observed in the exofacial coils. We identified six potential antigenic regions: one that is unique to RhD (R3), one that is common to all D (R6), three that are highly variable among RhD isoforms (R1, R2 and R4), one that they share with Rhce (R5) and two that are unique to Rhce (Ra and Rbc).ConclusionThe alloimmunization capacity of these subjects could be explained by the variability of the antigen pattern, which is not necessarily recognized or recognized with lower intensity by the commercially available antibodies, and not because they have a lower protein concentration in the membrane.