bluuebunny/crossref_metadata_2025_split huggingface dataset:
3‐D viscous Cahn–Hilliard equation with memory
Authors: Conti Monica , Mola Gianluca 
Published: November 2008

AbstractWe deal with the memory relaxation of the viscous Cahn–Hilliard equation in 3‐D, covering the well‐known hyperbolic version of the model. We study the long‐term dynamic of the system in dependence of the scaling parameter of the memory kernel ε and of the viscosity coefficient δ. In particular we construct a family of exponential attractors, which is robust as both ε and δ go to zero, provided that ε is linearly controlled by δ. Copyright © 2008 John Wiley & Sons, Ltd.
High‐pressure NMR characterization of triacetyl‐β‐cyclodextrin in supercritical carbon dioxide
Authors: Ivanova G. I. , Vão E. R. , Temtem M. , Aguiar‐Ricardo A. , Casimiro T. , Cabrita E. J. 
Published: November 2008

AbstractCyclodextrins are used in many drug formulations since their cavities provide microenvironments where drug molecules can enter and form inclusion complexes for controlled drug delivery. Supercritical carbon dioxide (scCO2) is an alternative to organic solvents and a very attractive medium for the preparation of these inclusion complexes. The potential ability of triacetyl‐β‐cyclodextrin (TA‐β‐CD) to form inclusion complexes in addition to its high miscibility in liquid and scCO2 could offer a chance for an economical and environmental friendly chemical processing. In this work, high‐pressure NMR studies were performed in order to obtain information on the molecular structure and dynamics of TA‐β‐CD in scCO2 at 313.15 K and 20 MPa and its ability to form inclusion complexes under these conditions was studied. The influence of scCO2 on a number of NMR spectral parameters, such as chemical shifts, spin‐spin coupling constants, nuclear Overhauser effect (NOE) and spin‐lattice relaxation (T1) has been studied. We unequivocally show for the first time structural changes of TA‐β‐CD in scCO2, like acetyl chain orientation and overall shape distortions that can affect its inclusion capability in this medium. The possibility of cavity self‐closure is discussed and the results of two inclusion studies that support cavity self‐closure, with the angiotensin‐converting enzyme inhibitor, captopril, and the nonsteroid anti‐inflammatory drug, flufenamic acid, are presented. Copyright © 2008 John Wiley & Sons, Ltd.
Fatigue as the presenting symptom of chronic inflammatory demyelinating polyneuropathy
Authors: Bissay Véronique , Flamez Anja , Schmedding Eric , Ebinger Guy 
Published: November 2008

AbstractDifferent clinical presentations of chronic inflammatory demyelinating polyneuropathy (CIDP) have been described. Fatigue is generally considered to be a secondary sign and is not mentioned as a warning sign for the diagnosis. We present a patient with CIDP in whom fatigue remained the only symptom, hereby stressing the importance of adding this disease to the differential diagnosis of fatigue. Immunomodulatory treatment did not change the clinical course of the patient, but electrodiagnostic features improved substantially. Muscle Nerve, 2008
Comparing the force– and excursion– frequency relationships in human skeletal muscle
Authors: Gregory Chris M. , Bickel C. Scott , Sharma Nitin , Dixon Warren E. 
Published: November 2008

AbstractWe examined the influence of varying stimulation frequency on muscle output during isometric and dynamic contractions. Our findings demonstrate that the predictability of the force– and excursion–frequency relationships is extremely strong across stimulation intensities. There were no differences in the frequency which elicited 50% of peak or peak muscle output. We conclude that the impact of varying stimulation frequency is consistent between isometric and dynamic contractions. Muscle Nerve, 2008
Asymptomatic Becker muscular dystrophy in a family with a multiexon deletion
Authors: Ferreiro Verónica , Giliberto Florencia , Muñiz García M. Noelia , Francipane Liliana , Marzese Diego M. , Mampel Alejandra , Roqué María , Frechtel Gustavo D. , Szijan Irene 
Published: November 2008

AbstractWe report a Becker muscular dystrophy (BMD) family with one 5‐year‐old affected patient and a 69‐year‐old asymptomatic grandfather. Dystrophin gene multiplex polymerase chain reaction and multiplex ligation‐dependant probe amplification analysis showed that both males carried an in‐frame deletion of exons 45–55. Segregation analysis revealed two additional asymptomatic boys in this family. Our finding supports previous predictions that exons 45–55 are the optimal multiexon skipping target in antisense gene therapy to transform the severe Duchenne muscular dystrophy into the milder BMD, or even asymptomatic, phenotype. Muscle Nerve, 2008
The effect of familial aggregation on the children with primary nocturnal enuresis
Authors: Wang Qing Wei , Wen Jian Guo , Zhu Qing Hua , Zhang Guo Xian , Yang Ke , Wang Yan , Zhu Zhi Qiang , Li Zhen Zhen , Zhang Rui Li , Yang Yan Feng , Wei Jin Xing 
Published: November 2008

AbstractObjectiveTo evaluate the effect of familial aggregation on the children with PNE by evaluating nocturnal urine output, bladder, and arouse function.Patients and MethodsAccording to whether relatives of family of probands over three generations were affected by PNE, forty‐five children with familial aggregation PNE (FPNE), seventy children with sporadic PNE (SPNE) and ten children with normal lower urinary tract function but waiting for operation (control group) were included. Questionnaire of arousal from sleep (AS scores), bladder diary and daytime urodynamic studies were performed in all patients.ResultsThe incidences of severe PNE and nonmonosymptomatic PNE in FPNE group were significantly higher than those in SPNE group. The nocturnal urine output and AS scores in both PNE groups was significantly higher, maximal voided volume significantly smaller than those in control group. Moreover, the incidences of small bladder in FPNE group was 44%, significantly higher than that in SPNE group (21%), but no significantly difference was found in nocturnal polyuria and arousal AS scores between two PNE groups. There were 53% patents with daytime detrusor overactivity and 60% patents with urodynamic functional bladder outflow obstruction in FPNE group, significantly higher than those in SPNE group (19% and 37%). Maximum cystometric capacity significantly decreased from control group to FPNE group.ConclusionFamilial aggregation has significant effects on the children with PNE, and FPNE are more likely to be severe symptoms and bladder dysfunction. It would be beneficial to have an urodynamic study for their diagnosis and treatment. Neurourol. Urodynam. 28:423–426, 2009. © 2008 Wiley‐Liss, Inc.
Can holidays boost consumption? The case of Hong Kong
Authors: Ramasamy Bala , Yeung Matthew C. H. , Au Alan K. M. 
Published: November 2008

Abstract
Hong Kong's domestic economy has been through a roller coaster ride over the past decade. In early 2004, increasing the number of holidays as a strategy to boost domestic consumption was mooted. In this paper, we estimate the effect of holidays on private domestic consumption in Hong Kong. We do this by estimating both the long term and short‐term consumption functions. Our estimates suggest that an additional day holiday per quarter would increase private consumption expenditure (PCE) by HKD 213 on a per capita basis. Although an extra day holiday could add 0.34% to GDP, policy makers should also consider the possible costs (e.g. cost of unproductive) of such a policy.
Copyright © 2008 John Wiley & Sons, Ltd.
Bush, Blair and Hitler? A review of comparative self‐presentation
Authors: O'Shaughnessy Nicholas Jackson 
Published: November 2008

Abstract
This article (while emphatic in stressing the absence of any political or ethical connection between two recent governments, those of George W. Bush and Tony Blair, and one historic one, the genocidal empire of Adolf Hitler), argues that the Nazi regime did in fact anticipate many communications methods pursued subsequently. The Nazis sponsored a communications revolution. The conscious production of political imagery and tableaux, government via symbolic strategies, the rhetoricizing of civic culture and the centrality of the rhetorical vision, all these have become part of our political truth as well as the Nazis'. Such a reality, then as now, is underpinned by spin, rapid rebuttal, duplicity and the construction of a nirvana of statistics and benevolent data. The ultimate fate of all these regimes was bound up with an ethos of government that placed symbolism centre‐ stage; a parallel universe of imagery and symbolism is no basis on which to construct an edifice of permanence. The methodologies of these regimes do sustain comparison, the ends, of course, do not.
Copyright © 2008 John Wiley & Sons, Ltd.
Why we will miss Ye, Teddy
Authors: Sommer Michael 
Published: November 2008

Abstract
In this commentary, written by senior commentator and presidential advisor Michael Sommer, who served all three Kennedy brothers, the author argues that the dying Senator Ted Kennedy has always truly fought for all Americans, has opponents but no enemies, that the world knows it, and that his liberalism is “the best American political philosophy” that America has. The author maintains that the Senator “represents the best that is in Americans, what America really stands for,” and that the world recognizes that, too. The author believes that President‐elect Obama will once again vigorously show the world the same Kennedy‐like enabling vision, hope, great leadership and true American spirit, that the world has tragically missed from America for the past eight years.
Copyright © 2008 John Wiley & Sons, Ltd.
HPLC‐MS analysis of toxic norditerpenoid alkaloids: refinement of toxicity assessment of low larkspurs (Delphinium spp.)
Authors: Gardner Dale R. , Pfister James A. 
Published: November 2008

AbstractIntroductionThe low larkspurs (Delphinium nuttallianum and D. andersonii) are two toxic plant species that are often fatally ingested by cattle on western USA rangelands. To assess the potential toxicity of the plants, methods are needed to identify and quantify the toxic N‐methylsuccinimidoanthranoyllycacontine type alkaloids in the plant.ObjectiveTo compare normal‐phase and reverse‐phase HPLC‐MS methods of analysis for detection and identification of toxic alkaloids in two species of toxic larkspur plants and to define the toxic alkaloids found in Delphinium nuttallianum and D. andersonii collected from several sites in the western USA.ResultsThe major toxic alkaloids found in the low larkspurs included methyllycaconitine, nudicauline, 14‐deacetylnudicauline and geyerline. Other toxic alkaloids detected at lower concentrations included 16‐deacetylgeyerline, grandiflorine, bearline, 14‐acetylbearline, barbinine, 16‐demethylnudicauline and three additional isomers of bearline. Total toxic alkaloid concentrations ranged from 0.72 mg/g (d.w.) to 7.02 mg/g determined by reverse‐phase HPLC‐MS.ConclusionThe low larkspurs contain a number of toxic alkaloids in addition to the alkaloid methyllycaconitine that need to be assessed when considering the toxicity of the plant. Both normal‐phase and reverse‐phase HPLC methods are adequate to detect and quantify the alkaloids. The reverse‐phase separation may be preferred due to readily available columns, reduced solvent use and simplicity of the electrospray ionisation source. Copyright © 2008 John Wiley & Sons, Ltd.
Clinical use of array comparative genomic hybridization (aCGH) for prenatal diagnosis in 300 cases
Authors: Van den Veyver Ignatia B. , Patel Ankita , Shaw Chad A. , Pursley Amber N. , Kang Sung‐Hae L. , Simovich Marcia J. , Ward Patricia A. , Darilek Sandra , Johnson Anthony , Neill Sarah E. , Bi Weimin , White Lisa D. , Eng Christine M. , Lupski James R. , Cheung Sau Wai , Beaudet Arthur L. 
Published: November 2008

AbstractObjectiveTo evaluate the use of array comparative genomic hybridization (aCGH) for prenatal diagnosis, including assessment of variants of uncertain significance, and the ability to detect abnormalities not detected by karyotype, and vice versa.MethodsWomen undergoing amniocentesis or chorionic villus sampling (CVS) for karyotype were offered aCGH analysis using a targeted microarray. Parental samples were obtained concurrently to exclude maternal cell contamination and determine if copy number variants (CNVs) were de novo, or inherited prior to issuing a report.ResultsWe analyzed 300 samples, most were amniotic fluid (82%) and CVS (17%). The most common indications were advanced maternal age (N = 123) and abnormal ultrasound findings (N = 84). We detected 58 CNVs (19.3%). Of these, 40 (13.3%) were interpreted as likely benign, 15 (5.0%) were of defined pathological significance, while 3 (1.0%) were of uncertain clinical significance. For seven (∼2.3% or 1/43), aCGH contributed important new information. For two of these (1% or ∼1/150), the abnormality would not have been detected without aCGH analysis.ConclusionAlthough aCGH‐detected benign inherited variants in 13.3% of cases, these did not present major counseling difficulties, and the procedure is an improved diagnostic tool for prenatal detection of chromosomal abnormalities. Copyright © 2008 John Wiley & Sons, Ltd.
OmniPlex—a new QF‐PCR assay for prenatal diagnosis of common aneuploidies based on evaluation of the heterozygosity of short tandem repeat loci in the Czech population
Authors: Putzova Martina , Pecnova Lubomira , Dvorakova Lucie , Soldatova Inna , Goetz Petr , Stejskal David 
Published: November 2008

AbstractObjectivesThe aim of our study was to assess the utility of commonly used multiplex assays of short tandem repeat markers used for quantitative fluorescent polymerase chain reaction (QF‐PCR) for prenatal rapid aneuploidy detection (RAD) in routine prenatal diagnosis in the Czech population.MethodsTwo previously published RAD multiplexes (2M test) were tested on 2906 local prenatal samples and used to calculate the rates of heterozygosity within this population. Most of the markers used in both multiplexes were highly informative. However, some had little utility, either due to a low heterozygosity rate (D21S499, D18S978 and P39) or because they were difficult to evaluate (DXS1283E).ResultsAfter evaluation of the 2M test results, a new multiplex assay (OmniPlex) was designed, developed and tested on 960 samples. This new assay was evaluated for heterozygosity rates and for the probability of having two or more informative markers on each chromosome.ConclusionsOmniPlex assay significantly improved the QF‐PCR methodology for rapid prenatal aneuploidy detection in the Czech population. Based on detected heterozygosity of markers used for QF‐PCR in this population, OmniPlex is a robust assay for the detection of chromosomes 13, 18, 21, X and Y in a single reaction. Copyright © 2008 John Wiley & Sons, Ltd.
Stem cell markers: Insights from membrane proteomics?
Authors: Ahn Sung‐Min , Goode Robert J. A. , Simpson Richard J. 
Published: November 2008

AbstractStem cells (SCs) are defined by their combined abilities to both self‐renew indefinitely in vitro and differentiate into adult cell types. One of the major driving forces of SC research is that SCs may provide a potentially unlimited source for cell‐replacement therapies in regenerative medicine. However, the identification of SCs and their progenies at different stages, and the success of cell‐replacement therapies, which form the basis of SC engineering, will depend on the ability to characterize and ultimately isolate homogeneous primary stem or progenitor cell populations to a large degree. Furthermore, the recent identification of cancer stem cells (CSCs) opens a new avenue for developing novel therapeutic strategies by targeting a specific subset of cancer cells with self‐renewal and proliferation capacity. Crucial to these tasks will be the discovery of novel plasma membrane‐associated SC markers. In this review, we focus on the seminal contribution that membrane proteomics could make to further clinical applications of SCs by providing tools for purification and identification of SCs and their progenies at each stage of differentiation, as well as, to understand the underlying mechanisms of SC differentiation. The need to standardize biological SC models before embarking on international SC proteomics efforts is discussed.
Proneurotensin/neuromedin N secreted from small cell lung carcinoma cell lines as a potential tumor marker
Authors: Ogura Shun‐ichiro , Kaneko Kumi , Miyajima Shoji , Ohshima Keiichi , Yamaguchi Ken , Mochizuki Tohru 
Published: November 2008

AbstractProteins secreted from specific cancer cells have a high potential for use as tumor markers. We identified secreted proteins produced by 15 different carcinoma cell lines grown in serum‐free medium using MS/MS. Proneurotensin/neuromedin N (proNT/NMN) was found in conditioned medium from four of seven small cell lung carcinoma cell lines but not from eight nonsmall cell lung carcinoma cell lines. These results indicate proNT/NMN has potential as a specific tumor marker of small cell lung carcinoma.
Proteome analysis of human foetal, aged and advanced nuclear cataract lenses
Authors: Hains Peter G. , Truscott Roger J. W. 
Published: November 2008

AbstractThe most complete proteome of human lenses has been compiled using 2‐D LC‐MS/MS analysis of foetal, aged normal and advanced nuclear cataract lenses. A total of 231 proteins were identified across all lens groups, including 112 proteins that have not been reported previously. Proteins were grouped according to their PANTHER molecular function classification in order to facilitate comparisons. Previously unreported N‐terminal acetylation was detected in a number of proteins, with the majority being associated with the prior removal of a methionine residue. This pattern of proteolysis may indicate that methionine aminopeptidase activity is present in human lenses. Acetylation is likely to aid in the stability of proteins that are present in the lens for many decades. Protein sequences were also used to interrogate the three human lens cDNA libraries publicly available. Surprisingly, 84 proteins we identified were not present in the cDNA libraries.
Her2 crosstalks with TrkA in a subset of prostate cancer cells: Rationale for a guided dual treatment
Authors: Festuccia Claudio , Gravina Giovanni Luca , Muzi Paola , Millimaggi Danilo , Dolo Vincenza , Vicentini Carlo , Ficorella Corrado , Ricevuto Enrico , Bologna Mauro 
Published: November 2008

AbstractBACKGROUNDTo date, no effective therapeutic treatment prevents prostate cancer (PCa) progression to more advanced and invasive disease forms. It has been demonstrated that the simultaneous high expression of p185HER2 and TrkA might confer a proliferative advantage to PCa cells.METHODSIn this work we verified the crosstalk between TrkA and Her2 signaling pathways and the effects of a combined treatment with Her2 and TrkA inhibitors.RESULTSNGF induced TrkA activation and stimulated cell proliferation of PCa cells. NGF induced also tyrosine phosphorylation of p185HER2. This event was only partially inhibited by the pan Trk inhibitor, CEP‐701 but was strongly blocked by pertuzumab, a humanized antibody blocking Her2 heterodimerization. In presence of NGF, TrkA and Her2 co‐precipitated and this was dependent to the relative high cellular levels of TrkA since when cell lysates were immunoprecipitated with an antibody against Her2 the amount of TrkA were proportional to the cellular levels of this receptor. On the contrary when we immunoprecipitated using an antibody against TrkA the amount of Her2 seemed independent to cellular levels of Her2. So, combined treatment between CEP‐701 and pertuzumab showed supra‐additive effects in cells with higher levels of TrkA and Her2 suggesting once again that this was indicative of a higher response to treatment.RESULTSOur data suggest that the dual inhibition of TrkA and Her2 may be useful in a subset of patients in which TrkA and Her2 are overexpressed and in which the possibility of TrkA and Her2 protein‐binding is elevated. Prostate 69:337–345, 2009. © 2008 Wiley‐Liss, Inc.
Survival in surgically treated, nodal positive prostate cancer patients is predicted by histopathological characteristics of the primary tumor and its lymph node metastases
Authors: Fleischmann Achim , Schobinger Sylviane , Schumacher Martin , Thalmann George N. , Studer Urs E. 
Published: November 2008

AbstractBACKGROUNDHistopathological risk factors for survival stratification of surgically treated nodal positive prostate cancer patients are poorly defined as reflected by only one category for nodal metastases.METHODSWe evaluated biochemical recurrence‐free survival (RFS), disease‐specific survival (DSS), and overall survival (OS) in 102 nodal positive, hormone treatment‐naïve prostate cancer patients (median age: 65 years, range: 45–75 years; median follow‐up 7.7 years, range: 1.0–15.9 years) who underwent radical prostatectomy and standardized extended lymphadenectomy.RESULTSA significant stratification was possible, with the Gleason score of the primary and virtually all nodal parameters favoring patients with better differentiated primaries and metastases, lower nodal tumor burden, and without extranodal extension of metastases. In multivariate analyses, diameter of the largest metastasis (≤10 mm vs. >10 mm) was the strongest independent predictor for RFS (P < 0.001), DSS (P < 0.001), and OS (P < 0.001) with a more than quadrupled relative risk of cancer related deaths for patients with larger metastases (Hazard ratio: 4.2, Confidence interval: 2.0–8.9; 5‐year RFS/DSS/OS: 18%/57%/54%). The highest 5‐year survival rates were seen in patients with micrometastases only (RFS/DSS/OS: 47%/94%/94%).CONCLUSIONThe TNM classification's current allocation of only one category for nodal metastases in prostate cancers is unsatisfactory since subgroups with significantly different prognoses can be identified. The diameter of the patient's largest metastasis (≤10 mm vs. >10 mm) should be used for substaging because of its independent prognostic value. The substage “micrometastasis only” is also useful in nodal positive prostate cancer since it designates the subgroup with the most favorable outcome. Prostate 69:352–362, 2009. © 2008 Wiley‐Liss, Inc.
Spatially resolved Raman piezo‐spectroscopic stress analysis in CaMoO4 thin film grown on Si substrate
Authors: Li Runtao , Zhu Wenliang , Ge Wanyin , Pezzotti Giuseppe 
Published: November 2008

AbstractConfocal piezo‐spectroscopy was applied to analyze the residual stress distribution developed in a CaMoO4‐film/Si‐substrate system, based on the spectroscopic shift upon stress of the 879 cm–1 Ag Raman mode of CaMoO4. In the stress characterization, use was made of spectral line scans, as well as laser defocusing measurements. A preliminary spectroscopic calibration of the Raman band was performed with using a ball‐on‐ring flexural bending jig. As a result, a value of –1.4 cm–1/GPa was found for the biaxial piezo‐spectroscopic coefficient of the Ag Raman mode of CaMoO4. A probe deconvolution procedure was introduced in the effort to correct the convoluting effect due to the finite size of the laser probe, taking advantage of the measurement of the probe response function. A theoretical analysis of the edge‐stress effect was then carried out and, accordingly, the actual in‐depth stress distribution was deduced from the observed spectral variation. Compressive residual stresses appeared on the CaMoO4 film side, while tensile residual stress occurred in the silicon substrate, and both stress magnitudes pronouncedly decreased with increasing distance from the interface. The results indicated relief in lattice mismatch in the epilayer, and such a relaxation mechanism was discussed in the paper. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)