bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic significance of DNA‐analysis by flow cytometry in non‐Hodgkin's lymphoma
Authors: Roos Göran , Dige Ulrik , Lenner Per , Lindh Jack , Johansson Hemming 
Published: February 2007

AbstractDNA analysis by flow cytometry was performed on lymph node cells obtained from 65 untreated patients with non‐Hodgkin's lymphoma. According to the Kiel classification 41 cases were of low grade malignancy and 24 cases belonged to the high grade malignancy group. 47 out of 61 evaluable cases were diploid/near‐diploid, 12 were aneuploid and 2 cases showed polyploidy. No difference in survival was found between diploid/near‐diploid and aneuploid cases.The percentage of S‐phase cells was found to be a valuable prognostic parameter. Using a cut point at 4 per cent S‐phase cells between low and high S‐phase lymphomas a highly significant difference in survival was demonstrated (p = 0.0004). The actuarial survival up to 3 years was about 77 per cent for lymphomas with <4 per cent S‐phase cells to be compared with 20 per cent for patients with high S‐phase (≥ 4 per cent) lymphomas. Subdivision according to the percentage of S‐phase cells seemed to be a better prognostic predictor than morphologic subclassification.
Neoplastic and reactive follicles within B‐cell malignant lymphomas. A morphological and immunological study of 30 cases
Authors: Pileri Stefano , Rivano Maria T. , Gobbi Marco , Taruscio Domenica , Lennert Karl 
Published: February 2007

AbstractThirty cases originally diagnosed as B‐cell lymphomas, either LP immunocytoma, centrocytic (Cc), or centroblastic‐centrocytic (Cb/Cc), containing follicular structures of an uncertain nature were critically reviewed using both morphological criteria and immunological techniques. In particular, they were tested by conventional antisera for detection of immunoglobulins in paraffin sections and also, in the 5 cases in which frozen material was available, by a panel of monoclonal antibodies.At the first histological evaluation the cases were divided into two groups. Group A consisted of 12 examples which showed homogeneous features and, because of the neoplastic nature of the follicular structures, could be classified as follicular centroblastic‐centrocytic lymphomas with marked plasmacellular differentiation. Group B comprised 18 cases which at onset of disease revealed a mantle‐fashion growth around reactive‐appearing follicles formed by polytypic germinal centre cells. On closer examination, however, this group appeared heterogeneous: (1) 13 cases displayed cytological and immunological findings consistent with the diagnosis of Cc and usually contained polytypic plasma cells; (2) 5 cases were examples of LP immunocytoma consisting of monotypic elements only. Therefore, the so‐called mantle‐zone lymphoma does not appear to be an entity.
Surface immunoglobulin pattern of the leukaemic cell population in chronic lymphocytic leukaemia (CLL) in relation to disease activity
Authors: Kimby E. , Mellstedt H. , Björkholm M. , Holm G. 
Published: February 2007

AbstractThe surface membrane immunoglobulin (smIg) isotype pattern of the leukemic lymphocytes was studied in 66 unselected patients with CLL. Five distinct patient groups were identified according to the dominant heavy chain isotype(s); I: smμ+ (n = 22), II: smμ+/smδ+ (n = 25), III: smδ+ (n = 4), IV: smγ+ (n = 5), V: no detectable heavy chains (n = 10). The majority of group I patients had a progressive disease at test while all patients in group II were in a non‐progressive state. Moreover, the smIg pattern changed with the clinical activity of the disease: when the disease progressed, the relative number of smμ+ cells increased and when patients entered an indolent stage after treatment the smμ+ cell population decreased. In patients with stationary disease the smIg pattern remained essentially unchanged or the relative number of smδ+ cells increased. These observations might suggest that the smIg isotype pattern of the leukemic cell population reflects the biological behaviour and the clinical activity of the disease.
Phenotypic characterization of ‘non‐T, non‐B’ acute lymphoblastic leukemia by a new panel (BL) of monoclonal antibodies
Authors: Al‐Katib Ayad , Wang Chang Yi , Bardales Ricardo , Koziner Benjamin 
Published: February 2007

AbstractPeripheral blood and/or bone marrow leukemic cell suspensions from 49 patients with ‘non‐T, non‐B’ acute lymphoblastic leukemia (ALL) were analysed by flow cytometry using a new panel of four monoclonal antibodies. Anti‐BL1 and anti‐BL2 originating from NALM‐6 and B35M lymphoblastoid cell lines, respectively. These antibodies recognize B‐cell differentiation antigens: a heat stable non‐immunoprecipitable antigenic determinant, and a 68 000 daltons glycoprotein molecule, respectively. BL5 and BL6 were derived by immunization with the promyelocytic cell line HL‐60, recognizing antigens present on early hemato‐poietic cells: an 85 000 daltons MW glycoprotein (Pro‐Im1) and a heat stable antigen (Pro‐Im2), respectively. All ALL patients studied had L1 or L2 morphology by the FAB classification and a blast count exceeding 50 per cent. There were 25 males and 24 females. Median age was 8 years (range 1–67 years). Thirty‐nine cases were studied at initial presentation and 10 at relapse. Cells from 46/49 cases expressed BL2 and/or BL1, but were not reactive with BL5 or BL6. Three of 49 cases did not express BL1 or BL2. However, a small percentage of blasts from one case was positive for BL5 (13 per cent) and the other 2 cases were reactive with BL6 (20 per cent and 36 per cent, respectively). These were one adult and 2 pediatric patients that had other ALL markers and achieved a complete remission with appropriate ALL therapy. One of the BL6+ cases relapsed after 19 months with a change in phenotype to BL1+ BL2+ BL5‐ BL6‐. This analysis shows that the majority of ‘non‐T, non‐B’ ALL's do express B‐cell associated antigens (BL1/BL2) argumentative of their B‐cell origin. A small subgroup does not express such antigens and may arise from a more immature cell, since they expressed antigens on early hematopoietic stem cells.
Molecular approach to adult t‐cell leukemia
Authors: Hatanaka Masakazu 
Published: February 2007

AbstractThe incidence of various types of hematological neoplasms varies with race. Among the lymphoid malignancies, B‐cell lymphomas and leukemias predominate in most races, but not in Japanese. For instance, chronic lymphocytic leukemia (B‐CLL) occurs rarely in Japanese as compared to other races. However, well‐characterized T cell malignancies occur in Japanese adults, including Sézary syndrome, mycosis fungoides and adult T‐cell leukemia. Adult T‐cell leukemia (ATL) was proposed by Uchiyama et al. (1977), to be a new disease entity distinct from other known malignancies. Subsequently much attention has been paid to this disease in Japan, because of its characteristic neoplastic cell morphology, clinical and pathological features and the endemic distribution in south‐western Japan. Hanaoka 's group characterized the cytological and histological features of ATL (Hanaoka et al., 1982). With the accumulation of pathological and clinical findings in cases of ATL and non‐leukemic pleomorphic T‐cell lymphoma (one type of malignant T‐cell lymphoma) these two diseases were included in one category called adult T‐cell leukemia/lymphoma (ATLL). However, the abbreviation ATL, not ATLL, will be used in this review article for simplicity, unless otherwise stated.
Familial disposition of adult t‐cell leukemia and lymphoma
Authors: Ichimaru Michito , Kinoshita Kenichiro , Kamihira Shimeru , Ikeda Shuichi , Yamadia Yasuaki , Suzuyama Junji , Momita Saburo , Amagasaki Tatsuhiko 
Published: February 2007

AbstractSixteen families, each with two or more cases of adult T‐cell leukemia or lymphoma were found in the Nagasaki district. Eight of the families had a parent with lymphoma. In the other eight families siblings were involved. Four families with sibling cases are presented in detail. Antibody titres to adult T‐cell leukemia associated antigen (ATLA) in cases of ATL, CTL, T‐CLL, and pre‐ATL cases in Nagasaki were all positive. Of the non‐leukemic T‐cell malignant lymphoma 62.5 per cent were positive for antibody. The positive rate in healthy spouses and siblings of ATLL patients for ATLA antibody was high (67.5 per cent and 40 per cent respectively). The possibility of ATLV infection through spouses or from mother to child and the meaning of the high familial incidence of ATLL is discussed.
Pathology of peripheral t‐cell lymphomas and leukemias
Authors: Watanabe Shaw 
Published: February 2007

AbstractPeripheral T‐cell lymphomas and leukemias have distinct histological and cytological characteristics although there are a variety of histological patterns even in a single lymph node. Histologic features are altered by the progression of the disease. There is no significant association between histology and prognosisThe variations in histologic features and clinical manifestions of peripheral T‐cell lymphomas/leukemias can now be related to the presence of the retrovirus ATLV, and anti‐ATLV antibodies as well as to the immunophenotypes of the lymphoma cells utilizing monoclonal antibodiesChanging concepts of peripheral T‐cell lymphomas/leukemias are discussed.
Virus associated adult t‐cell leukemia (ATL) in Japan: Clinical, histological and immunological studies
Authors: Kikuchi Masahiro , Mitsui Tetsuji , Takeshita Morishige , Okamura Hideki , Naitoh Hideaki , Eimoto Tadaaki 
Published: February 2007

AbstractVirus associated adult T‐cell leukemia/lymphoma (ATLL), which includes both adult T‐cell leukemia (ATL) and its non‐leukemic counterpart (NLATL) was studied clinically, histologically, and immunologically. The disease usually occurred in the sixth decade in both sexes equally. The patients had a rapid clinical course with frequent leukemic changes, lymphadenopathy, hepatomegaly, and occasional skin rash. Bone marrow involvement with mild infiltration and hypercalcemia were more frequent in ATL than in NLATL. Histologically the disease was categorized as malignant lymphoma, diffuse pleomorphic type with cerebriform nuclear giant cells. The lymphoma was characterized by diffuse proliferation of tumor cells with irregular nuclear configurations, varying in size and shape, and the presence of giant cells with highly convoluted cerebriform nuclei. The giant cells seemed to be a diagnostic markerImmunologically, the tumor cells usually possessed the surface antigens recognized by OKT 3, 4, Leu 8 and anti‐Tac antibodies, indicating that they were lymphomas of helper/inducer peripheral T‐cells with the receptor for interleukin 2, but they demonstrated no helper/inducer functionsThe patients often died of opportunistic infections due to T‐cell dysfunction caused by the disease itself and strong chemotherapy.
Non‐hodgkin's lymphomas in Jordanians: A histopathological study of 231 cases
Authors: Tarawneh Musleh S. 
Published: February 2007

AbstractOur hospital admitted 231 patients with non‐Hodgkin's lymphomas between June 1976 and November 1984. Review of these cases revealed the following profile: ages ranged from 1 to 70 years (median: 30 years), 156 males, predominantly children and adolescents, and 75 females. Diffuse histiocytic lymphoma accounted for 40·6 per cent of all cases. Seventeen patients (7 per cent) had nodular lymphomas. Fifteen patients, predominantly male children and adolescents, had lymphoblastic lymphomas and showed high association with mediastinal mass (47 per cent). Extranodal lymphomas mostly of the diffuse histiocytic subtype were diagnosed in 100 cases (43·3 per cent) and most frequently situated in the small intestine (59 cases). Burkitt's lymphoma—32 patients (14 per cent)—presented most often in male patients during the first decade of life and as an abdominal intestinal disease (19 cases). There were four cases of Mediterranean abdominal lymphoma (IPSID). Non‐Hodgkin's lymphomas in Jordanians are mostly of the diffuse histiocytic type, are rarely nodular, have a high frequency of extranodal involvement and tend to occur in younger age groups. Mediterranean abdominal lymphoma is less frequent than previously thought. The use of both of the Rappaport and Lukes‐Collins classifications demonstrated a high degree of reproducibility.
An immunohistochemical and clinicopathological study of granulocytic sarcoma (‘chloroma’)
Authors: Muller Salli , Sangster G. , Crocker J. , Nar Paramjit , Burnett D. , Brown G. , Leyland M. J. 
Published: February 2007

AbstractA small series of granulocytic sarcomas (GS) or ‘chloromas’ has been studied by means of conventional histology and immunohistochemistry. The latter was found to be most useful in the diagnosis and characterization of these neoplasms, which are rare in Great Britain. Polytypic antisera to leukocyte elastase and cathepsin G have proved to be most useful, giving intense staining in all cases. These two markers are more specific than muramidase (lysozyme) and α‐antitrypsin, since, unlike the latter two, they do not stain histiocytic cells. The results of staining with two monoclonal antisera, AGF 4.48 and AGF 4.36, are also describedIn addition, the clinicopathological details of the seven cases are summarized and the literature is briefly surveyed.
Interferon induces proliferation in leukemic and normal B‐cell subsets
Authors: Robért Karl‐Henrik , Einhorn Stefan , Östlund Leif , Juliusson Gunnar , Biberfeld Peter 
Published: February 2007

Abstract3H‐thymidine incorporation following stimulation with interferon (IFN) in vitro was investigated in cell cultures from peripheral blood of patients with chronic lymphocytic leukemia (CLL), spleens from necro‐kidney transplants and healthy blood donors. It was demonstrated, that IFN can induce a proliferative response in some normal as well as leukemic B lymphocyte subsets. The responses were not T‐cell dependant. The results indicate, that B‐cell subsets that proliferate in the presence of IFN, are present in higher proportions in spleen than in peripheral blood, and that they constitute a portion of the leukemic blood lymphocyte pool in some patients with CLLWe have previously demonstrated, that IFN induces varying degrees of transformation and differentiation in blood lymphocytes from a majority of CLL patients. The functional characteristics of different B‐cell subsets, and their heterogenous distribution in leukemia, may be important for the results of IFN treatment in various malignant B‐cell disorders.
Factors affecting remission and survival in patients with advanced hodgkin's disease treated with MVPP
Authors: Wagstaff John , Steward William , Jones Mary , Deakin David , Todd Ian , Wilkinson Peter , Scarffe John Howard , Harris Martin , Crowther Derek 
Published: February 2007

AbstractOne hundred and fourteen patients with clinical or pathological stages IIIB and IV Hodgkin's disease have been treated with MVPP chemotherapy followed by radiotherapy to sites of previously bulky disease. The minimum follow‐up is 2 years with a median of 5 years. The overall remission rate was 92 per cent with 74 per cent achieving CR. A discriminant analysis showed that the presence of bulky disease was the only independent factor that predicted a lower chance of CR (66 per cent vs 82 per cent,P= 0·045). The 5‐year survival was 70 per cent overall and 85 per cent for CR patients. A Cox multivariate analysis demonstrated that stage III disease, age less than 36 years and female sex were all variables which independently predicted a more favourable prognosis in terms of overall survival. However a similar analysis for survival of CR patients showed that age less than 36 years and the absence of bulky disease were the only two factors to independently predict a more favourable outcome. Of 14 patients who did not receive the chemotherapy according to protocol 5 have relapsed compared to 7 of 70 who did. A Cox analysis confirmed that this variable was the only one of significant prognostic import in predicting relapse‐free survival. Using the Cox analyses we have been able to devise a scoring system which accurately predicts the outcome for these patients. This model may be useful in determining which patients have a worse prognosis following treatment with MVPP thus allowing more intensive therapy to be given to such patients while minimising treatment for those with favourable features.
Erythroleukemia cell lines hel and K‐562: Changes in isoenzyme profiles and morphology during induction of differentiation
Authors: Drexler Hans G. , Gaedicke Gerhard , Minowada Jun 
Published: February 2007

AbstractThe effects of the phorbol ester 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) and of hemin on the human erythroleukemia cell lines HEL and K‐562 were studied using the parameters cellular morphology, cell proliferation, positivity in the nitro‐blue tetrazolium (NBT)‐reduction test, adherence to surface, development of pseudopodia, and isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) separated by isoelectric focusing on horizontal polyacrylamide gels. TPA and hemin induced similar morphological changes in HEL and K‐562, erythrocyte‐like cells were seen to be the predominant population after exposure to hemin. Both TPA and hemin led to cessation of cell proliferation and to a dose‐dependent decrease in the number of viable nucleated cells. Whereas HEL cells treated with TPA became positive in the NBT‐reduction test, adhered to plastic surface and showed pseudopodia, HEL cells exposed to hemin and K‐562 cells cultured with TPA or hemin did not show these markers indicative of the macrophage‐cell lineage. TPA‐treated HEL cells exhibited isoenzyme patterns which were characteristically seen in cells of the monocyte‐macrophage complex. ‘Myeloid isoenzyme profiles’ were found in TPA‐treated K‐562 cells. As evidenced by the above described phenotypical changes, HEL cells appeared to differentiate along the macrophage and erythroid cell lineages. Induced K‐562 cells displayed myeloid and erythrocytic markers.
Myelodysplastic syndrome evolving to a mixed myeloid‐lymphoid leukaemia
Authors: San Miguel J. F. , Gonzalez M. , Del Ca≁tizo M. C. , López‐Borrasca A. 
Published: February 2007

AbstractBetween 25 to 50 per cent of MDS patients progress to an acute leukaemia, classically assumed to be of myeloid lineage (Tricot et al., 1985). However, recently two cases of MDS evolving to ALL have been described (Barton et al., 1980; Berneman et al., 1985).
Cytotoxic and DNA‐inhibitory effects of iron chelators on human leukaemic cell lines
Authors: Kontoghiorghes George J. , Piga Andrea , Hoffbrand A. Victor 
Published: February 2007

AbstractCytotoxic and DNA synthesis inhibitory effects of several iron chelators with different physicochemical properties have been tested in four myeloid leukaemic cell lines (U937, K562, ML2 and HL60). The small lipophilic chelators 8‐hydroxyquinoline, tropolone an5d omadine at 2 × 10−M, caused substantial inhibition of labelled leucine and thymidine uptake into cells and also cell death following 4‐h incubation. These effects were approximately 10‐fold increased when the drugs were pre‐incubated with equimolar amounts of iron. Iron alone and hydrophilic chelators e.g. desferrioxamine had insignificant cytotoxic and DNA synthesis inhibitory effects under the same conditions.
A cytological analysis of FMC‐7 positive leukaemias
Authors: Hart D. N. J. , Beard M. E. J. , Hamer J. W. , Heaton D. C. , Neville M. A. , Southern M. 
Published: February 2007

AbstractThe subdivision of the B lymphoid leukaemias by conventional techniques is subjective and poorly reproducible, with a range of cytological diagnoses available for cases which are not typical examples of chronic lymphatic leukaemia or acute lymphoblastic leukaemia. The monoclonal antibody FMC‐7 recognizes a determinant on a subpopulation of B lymphoid cell and stains follicular B cells. Routiune FACS analysis of chronic lymphoid leukaemias with a panel of monoclonal antibodies identified a subset of lymphoproliferative disorders (20 of 88) which were FMC‐7 positive. a careful ‘blind’ cytological assessment of this subset gave some support for the suggestion that they were examples of lymphoproliferative disease of follicular origin. Eight cases, however, were considered cytologically typical of CLL. The wider application of this antibody, particularly in sequential studies over a longer time scale may improve objectivity in the classification of this group of diseases.
Induction of differentiation in chronic myelocytic leukaemia cells by the phorbolester TPA
Authors: Drexler Hans G. , Bhoopalam Nirmala , Klein Mary , Gaedicke Gerhard , Minowada Jun 
Published: February 2007

AbstractThe mononuclear peripheral blood cells from eight patients with chronic myelocytic leukaemia (CML) were incubated in cell suspension culture in the presence of the phorbolester 12‐0‐tetradecanoylphorbol 13‐acetate (TPA). TPA caused the treated cells to adhere and to acquire morphological and functional features characteristic of macrophage‐like cells. Using isoelectric focusing distinct changes in the isoenzyme profiles of carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase were detected in the TPA‐exposed cells. Besides an increase in the number and staining intensity of isoenzymes of all enzymes, TPA triggered the new expression of a monocyte‐specific esterase isoenzyme and of the tartrate‐resistant acid phosphatase isoenzyme. The latter two isoenzymes represent further parameters of the monocyte/ macrophage complex. The results indicate that immature leukaemic cells arrested along the granulocytic cell axis retain the ability to transform to macrophages.
Expression of the T1 (CD5, p67) surface antigen in B‐CLL and B‐NHL and its correlation with other B‐cell differentiation markers
Authors: Delia Domenico , Bonati Antonio , Giardini Roberto , Villa Silvia , Braud Filippo De , Cattoretti Giorgio , Rilke Franco 
Published: February 2007

AbstractThe T1 surface antigen (CD5,p67) expression on blood lymphocytes (PBL) and lymphoid cells from lymph node biopsies (LN) from 31 patients with B‐cell chronic lymphocytic leukemia (B‐CLL) and 79 with B non‐Hodgkin lymphoma (B‐NHL), was detected in 25 B‐CLL (80 per cent) and in 11 B‐NHL (13 per cent) belonging to the following histologic subtypes: lymphocytic of CLL type (DLWD) one case, lymphoplasmacytoid (DLWD) four cases, centrocytic (DLPD) five cases, immunoblastic (DH) one caseAll B‐CLL and the T1 + B‐NHL were also tested with monoclonal antibodies against the Common Acute Lymphoblastic Leukemia Antigen, B cells (FMC7, FMC8, BA1, Y29–55), T cells (OKT11a), HLA‐DR and HLA‐DQ monomorphic determinantsAll the B‐CLL and the T1 + B‐NHL were CALLA‐, BA1+, Y29.55+ . FMC7+ cells were detected in large numbers six B‐CLL(three T1+ and three T1‐) and in four centrocytic lymphomas. FMC8 reacted with 70 per cent of leukemias (where it stained 30 per cent of neoplastic cells) and with 8/9 T1+ B‐NHL. HLA‐DR and HLA‐DQ molecules were detected in 100 per cent and 90 per cent of cases respectively. In vitro treatment of HLA‐DQ‐ or T1‐ B‐CLL with phorbol ester TPA led to the expression of these antigens as well as of the receptors for Interleukin 2 and MLR3 activation antigen. Surface membrane Ig (SIg) was detected in 79 per cent of cases, its density measured by FACS analysis varied, even markedly, from case to case. Among the B‐CLL, cells with high SIg content were either T1+ or T1‐ and more likely FMC+. The SIg‐ cases were seven B‐CLL (five T1+ and two T1‐) and two B‐NHL, in which, however, cytoplasmic IgM was detectedThis study reveals the existence of four major B‐CLL subgroups: T1‐ SIg‐, T1+ SIg‐, T1+ SIg+, T1‐ SIg+. It also indicates that the T1 antigen may be transitionally present during B‐cell differentiation and that its expression may precede that of SIg as supported by the in vitro studies. In addition, the finding that some B‐NHL are T1+ suggests that they derive similarly to the B‐CLL from a common progenitor.