bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anticipated uptake of genetic testing for familial melanoma in an Australian sample: an exploratory study
Authors: Kasparian Nadine A. , Meiser Bettina , Butow Phyllis N. , Soames Job R. F. , Mann Graham J. 
Published: July 2006

AbstractIntroduction: The potential role of genetic testing in families with an inherited pattern of melanoma is a complex issue, and yet limited data exist on perceptions of predictive genetic testing for mutations among individuals at high risk of melanoma.Methodology: Forty semi‐structured interviews were undertaken with affected and unaffected individuals at either high or average risk of developing melanoma due to family history. Interviews addressed key issues such as: the role of genetics in causal attributions for melanoma; genetic testing intentions and motivations; perceived accuracy of genetic testing in predicting melanoma onset, and the impact of varied accuracy on testing intentions; views on the testing of children; perceived benefits and limitations of testing; and information needs and communication preferences.Results: In‐depth thematic analysis revealed a number of important qualitative differences between groups at varying risk of melanoma, and genders. Specifically, participants with a family history of melanoma believed genetic factors play an important role in melanoma causation; conveyed strong intentions to pursue genetic testing; and viewed the benefits of genetic testing as outweighing the limitations. Females appeared to endorse the testing of children more firmly than males, and males' intentions to pursue testing appeared more contingent on penetrance than females'. Across groups, the most preferred communication option was an informational video.Conclusion: Those at high risk of melanoma due to family history express a strong interest in predictive genetic testing. Copyright © 2006 John Wiley & Sons, Ltd.
High pulse area undamping of Rabi oscillations in quantum dots coupled to phonons
Authors: Vagov A. , Croitoru M. D. , Axt V. M. , Kuhn T. , Peeters F. M. 
Published: July 2006

AbstractWe study the dynamics of strongly confined semiconductor quantum dots, coupled to acoustic phonons and driven by external laser pulses, by a numerical path integral method. Our method fully explores the relevant memory of the system thus accounting for all non‐Markovian features of the dynamics. The decay time and the frequency of Rabi oscillations are found to depend notably on the temperature and the applied field. The field dependence is non‐monotonic leading to an undamping of Rabi oscillations at high pulse areas. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Quantum optics with interacting polaritons
Authors: Savasta Salvatore , Stefano Omar Di 
Published: July 2006

AbstractRecent theoretical and experimental investigations evidencing quantum optical effects in semiconductor microcavities are reported. In particular the results of an experiment that probes polariton quantum correlations by exploiting quantum complementarity are described. In this experiment, it has been shown that polaritons in two distinct idler modes interfere if and only if they share the same signal mode so that which‐way information cannot be gathered, according to Bohr's quantum complementarity principle. The experimental results prove the existence of polariton pair correlations that store the which‐way information. This interpretation is confirmed by a theoretical analysis of the measured interference visibility in terms of quantum Langevin equations. The devised experimental scheme employs two frequency‐degenerate mutually‐coherent pump modes. It is shown that this kind of two‐pump parametric schemes can be exploited to realize a number of interesting tasks as the emission of polarization‐entangled cavity‐polariton states and the coherent control and trapping of parametric amplification in SMCs. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Memory and correlation effects in the exciton–phonon kinetics
Authors: Mannarini Gianandrea , Zimmermann Roland 
Published: July 2006

AbstractMemory and correlation effects in the interband absorption from quantum wells due to exciton–phonon dynamics are investigated. They are traced back to the frequency dependence and matrix character of the self energy arising in a 2nd Born theory. It is found that interpeak absorption increases with respect to the case in which this memory and correlation effects are neglected. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Quantum Stark confined strongly correlated indirect excitons in quantum wells
Authors: Ludwig P. , Filinov A. V. , Bonitz M. , Stolz H. 
Published: July 2006

AbstractIn this contribution we consider small ensembles of optically excited indirect excitons in a single quantum well. Using Path Integral Monte Carlo we compute from first principles the spatial separation of electrons and holes and the lateral quantum Stark confinement of the excitons in the quantum well which is produced by an external electric field of a single tip electrode. By changing the field strength, the tip to sample distance, the excitation intensity (exciton number) and temperature, the exciton–exciton correlations can be varied in broad ranges. Discussing two different semiconductor quantum well heterostructures (GaAs/AlGaAs and ZnSe/ZnSSe) we propose a possible setup which should allow to observe in experiments interesting many‐particle phenomena. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Autonomous excavation using a rope shovel
Authors: Dunbabin Matthew , Corke Peter 
Published: July 2006

AbstractThis paper describes the development of a system to automate the digging cycle of an electric rope shovel, a machine which is widely used in open‐pit mining. Achieving optimal digging performance requires path planning strategies to ensure dipper (bucket) filling, as well as methods to detect when to disengage the dipper from the bank. To this end, techniques to detect and avert dipper stall, and the online estimation of dipper “full‐ness,” are described along with in‐field experimental results using a one‐seventh scale‐model electric rope shovel. Over 100 autonomous excavation cycles, in a variety of digging conditions, resulted in cycle times consistent with a human operator, but with lower overall motor loading. © 2006 Wiley Periodicals, Inc.
A situational implementation method for web‐based content management system‐applications: method engineering and validation in practice
Authors: van de Weerd Inge , Brinkkemper Sjaak , Souer Jurriaan , Versendaal Johan 
Published: July 2006

AbstractThe usage of data‐intensive web applications raises problems concerning consistency, navigation, and data duplication. Content management systems (CMSs) can overcome these problems. In this research, we focus on special types of web content management systems—web‐based CMS applications. Currently, no general available methods exist for implementing and configuring these applications. In this research, an assembly based situational method engineering approach is proposed for constructing an implementation method for web‐based CMS applications. The approach consists of four steps: (a) identification of implementation situations, (b) selection of candidate methods, (c) analysis and storage of relevant fragments in the method base, and (d) assembly of the new method using route maps to obtain situationality. This method engineering approach is supported by a meta‐modeling technique, resulting in a process‐data diagram, which integrates UML (Unified Modeling Language) activity diagrams and class diagrams. To validate the method, two case studies were performed at a large health insurance organization and a telecommunication organization in the Netherlands. The new implementation method performed well in both case studies, and the project workers were satisfied with the associated templates and instructions. Copyright © 2006 John Wiley & Sons, Ltd.
Mobile virtual private networks with dynamic MIP home agent assignment
Authors: Chen Jyh‐Cheng , Liu Yi‐Wen , Lin Li‐Wei 
Published: July 2006

AbstractThis paper presents the dynamic external Home Agent (x‐HA) assignment in mobile Virtual Private Networks (VPNs). The proposed architecture is based on the mobile VPN proposed by the IETF, which adopts Mobile IP and IPsec. The IETF solution, however, leads to two questions: where should we put the x‐HA and how should we trust the x‐HA? We propose to assign the x‐HA dynamically so the handoff latency and end‐to‐end latency could be reduced significantly. By using Diameter Mobile IPv4 application, we also propose a technique such that the x‐HA can be associated with the VPN securely. In addition, the registrations with x‐HA and internal HA (i‐HA) are concurrently accomplished. The proposed technique has been implemented in a mobile VPN testbed. Performance analysis based on empirical experiments is discussed. Copyright © 2006 John Wiley & Sons, Ltd.
Fast movement detection in IEEE 802.11 networks
Authors: Montavont Nicolas , Noel Thomas 
Published: July 2006

AbstractThis paper proposes a solution framework for Mobile IPv6 to anticipate movement over IEEE 802.11 networks. Layer 2 triggers such as the signal strength is used to trigger in advance scanning phases, in which a mobile device probes the adjacent access points. This scanning phase is performed alternatively with communication. Sufficient Layer 3 information is conveyed in Layer 2 frames, which allows a mobile device to identify both its next Layer 2 and Layer 3 point of attachment. Simulation results show that this method performs well and enhance both the Layer 2 and the Layer 3 handover. The impact of handovers on real‐time application is also considerably minimized. Copyright © 2006 John Wiley & Sons, Ltd.
Mobile IPv6‐based efficient vertical handoff approach for heterogeneous wireless networks
Authors: Chang Ben‐Jye , Lin Shu‐Yu 
Published: July 2006

AbstractThe demand and amount of data transfer in heterogeneous wireless network increases as the techniques of the third/fourth generation mobile communications (3G/4G) are progressed significantly. Vertical handoff provides continuous and seamless transfer between various wireless mobile networks, which have different data transmission techniques and standards such as GPRS, UMTS, cdma2000 and IEEE 802.11 family WLANs. In contrast to the horizontal handoff, the vertical handoff should consider not only the strength of signal power but also QoS classes, network bandwidth and service types, etc. Most of the previous works proposed the received signal strength (RSS) based methods to set thresholds for making the decision of performing vertical handoff or not. The RSS‐based approach yields serious ping‐pong effect when the mobile node moves around the overlay area of two heterogeneous wireless networks, which causes unnecessary handoff and increases the handoff overhead. Although the RSS‐based approach with the hysteresis technique decreases the number of unnecessary handoffs, it suffers from large overhead of handoff and causes several drawbacks, including low network throughput, long handoff delay, and high dropping probability. Therefore, an efficient cost‐based vertical handoff algorithm is proposed herein to reduce unnecessary handoffs while increasing network throughput, decreasing handoff delay, and avoiding connection dropping. Two primary motivations of this paper include the prediction of node mobility from RSS and the adaptive cost‐based competitive on‐line (COL) method to determine the optimal network to handoff to from all available candidates. Furthermore, message flows of vertical handoffs between 3G/UMTS and WLAN as well as horizontal handoff between WLANs with MIPv6 are expressed in detail. Finally, the handoff delays are analyzed for the proposed MIPv6 handoff. Numerical results demonstrate that the proposed cost‐based approach outperforms other approaches in the number of vertical handoffs and connection dropping while yielding competitive throughput. Copyright © 2006 John Wiley & Sons, Ltd.
Reversible metabolic myopathy in biotinidase deficiency: its possible role in causing hypotonia
Authors: Bay C. A. , Berry G. T. , Glauser T. A. , Hayward J. C. , Wolf B. , Sladky J. T. , Kaplan P. 
Published: July 2006

SummaryA 5‐year‐old girl diagnosed with biotinidase deficiency at 9 months of age demonstrated limb and axial hypotonia which improved on biotin therapy. In this patient, electromyographic (EMG) studies prior to treatment were compatible with a mild myopathic process. Serial EMGs performed on biotin therapy demonstrated a gradual resolution of the myopathy. This is the first documented case of a reversible myopathy in a patient with biotinidase deficiency, which may contribute to the clinical finding of hypotonia.
Assignment of the human peroxisomal palmitoyl‐CoA oxidase gene to chromosome 17q23‐qter by PCR technique
Authors: Moghrabi N. N. , Dawson D. B. , Bennett M. J. 
Published: July 2006

SummaryHuman peroxisomal palmitoyl‐CoA oxidase plays a pivotal role in the β‐oxidation of fatty acids. Its importance is reflected by the severity of the disease associated with its deficiency in man. The gene was previously mapped to chromosome 17q25 with a FISH technique and is now confirmed using a PCR technique.
Pseudodeficiency of arylsulphatase A: Strategy for clarification of genotype in families of subjects with low ASA activity and neurological symptoms
Authors: Leistner S. , Young E. , Meaney C. , Winchester B. 
Published: July 2006

SummaryA benign deficiency (pseudodeficiency) of the lysosomal enzyme arylsulphatase A (ASA) (EC 3.1.6.8) towards synthetic substrates complicates the diagnosis of metachromatic leukodystrophy (MLD). The pseudodeficiency is due to a single base substitution in the 3′‐untranslated region of the ASA gene (1524+95 A→G) and it has been reported that this mutation (PD2) always occurs on a chromosome carrying a second mutation in the ASA gene (PD1), which abolishes anN‐glycosylation site (N350S). Analysis of the two PD mutations in the ASA gene separately was carried out in a large group of subjects with neurological symptoms and low ASA activity, including close relatives and MLD patients. The relationship between ASA enzyme activity and the different genotypes identified is presented. Evidence for the existence of an allele containing the PD2 mutation alone is presented. A strategy for cases with low ASA activity and neurological symptoms in families carrying a PD allele or both PD and MLD alleles is proposed.
Genotyping of a patient homozygous for a rare apolipoprotein E1 [Gly127→Asp; Arg158→Cys] (Weisgraber allele)
Authors: Iron A. , Richard P. , Pascual de Zulueta M. , Thomas G. , Thomas M. 
Published: July 2006

SummaryWe examined the apolipoprotein E polymorphism of an obese patient presenting non‐insulin‐dependent diabetes, hypertension and moderate lipid disturbances. The apolipoprotein E genotyping carried out from leukocyte DNA using PCR amplification and restriction enzyme digestion demonstrated homozygosity for the rare apoE1[Gly127→Asp; Arg158→Cys] (Weisgraber allele). The nucleotide change results in a glycine to aspartic acid substitution at amino acid 127 in the apolipoprotein E2.
Fatty acid content in lymphocytes from children with syndromic paucity of interlobular bile ducts, Alagille syndrome
Authors: Pina P. , Couturier M. , Lemonnier F. 
Published: July 2006

SummaryFatty acid (FA) concentrations were studied in lymphocytes isolated from children with syndromic paucity of interlobular bile ducts (PILBD), Alagille syndrome. The aim of this study was to assess whether the specific FA changes previously observed in fibroblast cultures from such patients were also present in other tissues. Lymphocyte FA, obtained both from controls and patients were studied under two experimental conditions, either after separation of the mononuclear cells or after 48 hours of culture. Freshly isolated lymphocytes from patients presented few FA changes compared to the controls. However, when patient lymphocytes were placed in culture medium for 47 hours, FA changes were amplified compared to those observed in controls; the decrease in the sum of saturated andn‐6 polyunsaturated FA of total lipids was significant only in patients, and then‐3 FA of phospholipids was strikingly increased in patients (p≤0.001), compared to controls. These results are related to those previously observed in fibroblast cultures and suggest that placing cells in culture could reveal a pre‐existing cellular abnormality in patients with PILBD.
Fabricating colloidal crystals and construction of ordered nanostructures
Authors: Sun Zhiqiang , Yang Bai 
Published: July 2006

Abstract
Colloidal crystals of polymeric or inorganic microspheres are of extensive interest due to their potential applications in such as sensing, optics, photonic bandgap and surface patterning. The article highlights a set of approaches developed in our group, which are efficient to prepare colloidal crystals with ordered voids, patterned colloidal crystals on non-planar surfaces, heterogeneous colloidal crystals of different building blocks, colloidal crystals composed of non-spherical polyhedrons, and colloidal crystals of non-close-packed colloidal microspheres in particular. The use of these colloidal crystals as templates for different microstructures range from nanoscale to micron-scale is also summarized.
Hypertonic saline‐induced muscle nociception and c‐fos activation are partially mediated by peripheral NMDA receptors
Authors: Ro Jin Y. , Capra Norman F. , Lee Jong‐Seok , Masri Radi , Chun Yang‐Hyun 
Published: July 2006

AbstractIn this study, the animal model of hypertonic saline (HS) infusion protocol was developed and utilized to test the hypothesis that HS causes peripheral release of glutamate, and that blockade of peripheral NMDA receptors significantly reduces HS‐induced nocifensive behavior and central neuronal activation. Nocifensive behavior and c‐fos immunoreactivity, as a marker of central neuronal activation, were assessed from the animals that received intramuscular HS infusion with and without the NMDA receptor antagonist, MK‐801. HS infusion (20μl/min for 10min) in the rat masseter produced prolonged nocifensive hindpaw shaking responses that peaked in the first minute and gradually diminished over the infusion period. The HS induced nocifensive behavior was dose‐dependently attenuated by MK‐801 pretreatments (0.3mg/kg and 0.1mg/kg), but not by vehicle pretreatment (isotonic saline; ISO), in the masseter muscle. HS infusion produced a significant number of Fos positive neurons in the ispsilateral subnucleus caudalis (Vc). Subsequent immunohistochemical studies showed that peripheral MK‐801 pretreatment effectively reduced the HS induced neuronal activation in the Vc. These results provide compelling evidence that HS‐induced muscle nociception is mediated, in part, by peripheral release of glutamate, and that blockade of peripheral glutamate receptors may provide effective means of preventing central neuronal activation.
Bistable switching and excitable behaviour in the activation of Src at mitosis
Authors: Fuß Hendrik , Dubitzky Werner , Downes Stephen , Kurth Mary Jo 
Published: July 2006

Abstract
Motivation: The protein tyrosine kinase Src is involved in a multitude of biochemical pathways and cellular functions. A complex network of interactions with other kinases and phosphatases obscures its precise mode of operation.
Results: We have constructed a semi-quantitative computational dynamic systems model of the activation of Src at mitosis based on protein interactions described in the literature. Through numerical simulation and bifurcation analysis we show that Src regulation involves a bistable switch, a pattern increasingly recognised as essential to biochemical signalling. The switch is operated by the tyrosine kinase CSK, which itself is involved in a negative feedback loop with Src. Negative feedback generates an excitable system, which produces transient activation of Src. One of the system parameters, which is linked to the cyclin dependent kinase cdc2, controls excitability via a second bistable switch. This topology allows for differentiated responses to a multitude of signals. The model offers explanations for the existence of the positive and negative feedback loops involving protein tyrosine phosphatase alpha (PTPα) and translocation of CSK and predicts a specific relationship between Src phosphorylation and activity.
Contact: fuss-h@ulster.ac.uk
Identification of metabolic units induced by environmental signals
Authors: Nacher Jose C. , Schwartz Jean-Marc , Kanehisa Minoru , Akutsu Tatsuya 
Published: July 2006

Abstract
Motivation: Biological cells continually need to adapt the activity levels of metabolic functions to changes in their living environment. Although genome-wide transcriptional data have been gathered in a large variety of environmental conditions, the connections between the expression response to external changes and the induction or repression of specific metabolic functions have not been investigated at the genome scale.
Results: We present here a correlation-based analysis for identifying the expression response of genes involved in metabolism to specific external signals, and apply it to analyze the transcriptional response of Saccharomyces cerevisiae to different stress conditions. We show that this approach leads to new insights about the specificity of the genomic response to given environmental changes, and allows us to identify genes that are particularly sensitive to a unique condition. We then integrate these signal-induced expression data with structural data of the yeast metabolic network and analyze the topological properties of the induced or repressed subnetworks. They reveal significant discrepancies from random networks, and in particular exhibit a high connectivity, allowing them to be mapped back to complete metabolic routes.
Contact: nacher@kuicr.kyoto-u.ac.jp, jean@kuicr.kyoto-u.ac.jp
An ontology for a Robot Scientist
Authors: Soldatova Larisa N. , Clare Amanda , Sparkes Andrew , King Ross D. 
Published: July 2006

Abstract
Motivation: A Robot Scientist is a physically implemented robotic system that can automatically carry out cycles of scientific experimentation. We are commissioning a new Robot Scientist designed to investigate gene function in S. cerevisiae. This Robot Scientist will be capable of initiating &gt;1,000 experiments, and making &gt;200,000 observations a day. Robot Scientists provide a unique test bed for the development of methodologies for the curation and annotation of scientific experiments: because the experiments are conceived and executed automatically by computer, it is possible to completely capture and digitally curate all aspects of the scientific process. This new ability brings with it significant technical challenges. To meet these we apply an ontology driven approach to the representation of all the Robot Scientist’s data and metadata.
Results: We demonstrate the utility of developing an ontology for our new Robot Scientist. This ontology is based on a general ontology of experiments. The ontology aids the curation and annotating of the experimental data and metadata, and the equipment metadata, and supports the design of database systems to hold the data and metadata.
Availability: EXPO in XML and OWL formats is at: . All materials about the Robot Scientist project are available at: .
Contact: lss@aber.ac.uk