bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumor-targeting nanodelivery enhances the anticancer activity of a novel quinazolinone analogue
Authors: Hwang Sung Hee , Rait Antonina , Pirollo Kathleen F. , Zhou Qi , Yenugonda Venkata Mahidhar , Chinigo Gary M. , Brown Milton L. , Chang Esther H. 
Published: March 2008

AbstractGMC-5-193 (GMC) is a novel anticancer small-molecule quinazolinone analogue with properties that include antimicrotubule activity and inherent fluorescence. The aim of this study was to produce and optimize a systemically administered liposomal formulation for tumor-targeting delivery of GMC to enhance the anticancer effect of this compound and evaluate its bioefficacy. GMC was encapsulated within a cationic liposome, which was decorated on the surface with an anti-transferrin receptor single-chain antibody fragment (TfRscFv) as the tumor-targeting moiety to form a nanoscale complex (scL/GMC). Confocal imaging of fluorescent GMC uptake in a human melanoma cell line, MDA-MB-435, showed higher cellular uptake of GMC when delivered via the liposome complex compared with free GMC. Delivery of GMC by the tumor-targeting liposome nanoimmunocomplex also resulted in a 3- to 4-fold decrease in IC50 values in human cancer cells [DU145 (prostate) and MDA-MB-435] compared with the effects of GMC administered as free GMC. In addition, the GMC nanoimmunocomplex increased the sensitivity of cancer cells to doxorubicin, docetaxel, or mitoxantrone by ∼3- to 30-fold. In the MDA435/LCC6 athymic nude mice xenograft lung metastases model, GMC was specifically delivered to tumors by the nanoimmunocomplex. These data show that incorporation of small-molecule therapeutic GMC within the tumor-targeting liposome nanocomplex enhances its anticancer effect. [Mol Cancer Ther 2008;7(3):559–68]
A chaperone protein-enriched tumor cell lysate vaccine generates protective humoral immunity in a mouse breast cancer model
Authors: Li Gang , Andreansky Samita , Helguera Gustavo , Sepassi Marjan , Janikashvili Nona , Cantrell Jessica , LaCasse Collin L. , Larmonier Nicolas , Penichet Manuel L. , Katsanis Emmanuel 
Published: March 2008

AbstractWe have documented previously that a multiple chaperone protein vaccine termed chaperone-rich cell lysate (CRCL) promotes tumor-specific T-cell responses leading to cancer regression in several mouse tumor models. We report here that CRCL vaccine generated from a mouse breast cancer (TUBO, HER2/neu positive) is also capable of eliciting humoral immunity. Administration of TUBO CRCL triggered anti-HER2/neu antibody production and delayed the progression of established tumors. This antitumor activity can be transferred through the serum isolated from TUBO CRCL-immunized animals and involved both B cells and CD4+ T lymphocytes. Further evaluation of the mechanisms underlying TUBO CRCL-mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results suggest that tumor-derived CRCL vaccine has a wider applicability as a cancer vaccine because it can target both T-cell- and B-cell-specific responses and may represent a promising approach for the immunotherapy of cancer. [Mol Cancer Ther 2008;7(3):721–9]
Antitumor effect of dsRNA-induced p21WAF1/CIP1 gene activation in human bladder cancer cells
Authors: Chen Zhong , Place Robert F. , Jia Zhe-Jun , Pookot Deepa , Dahiya Rajvir , Li Long-Cheng 
Published: March 2008

Abstract
We recently reported that synthetic dsRNAs targeting promoter regions can induce gene expression in a phenomenon referred to as dsRNA-induced gene activation/RNA activation (RNAa) [Li et al. Proc Natl Acad Sci U S A 2006;103:17337–42]. The present study investigates the in vitro antitumor activity RNAa can elicit through triggering the expression of cell cycle repressor protein p21WAF1/CIP1 (p21) in human bladder cancer cells. Transfection of a 21-nucleotide dsRNA targeting the p21 promoter (dsP21) was used to induce p21 expression in T24 and J82 bladder cancer cell lines. Reverse transcription-PCR and Western blot analysis accessed the increase p21 mRNA and protein levels, respectively, in transfected cells. In association to p21 induction, dsP21 transfection significantly inhibited bladder cancer cell proliferation and clonogenicity. Further analysis of cell viability and cell cycle distribution revealed that dsP21 transfection also enhanced apoptotic cell death and caused an accumulation in the G1 phase in both cell lines. In conclusion, p21 activation by RNAa has antitumor activity in vitro in bladder cancer cells. These results suggest that RNAa could be used for cancer treatment by targeted activation of tumor suppressor genes. [Mol Cancer Ther 2008;7(3):698–703]
Using Brain Imaging to Extract the Structure of Complex Events at the Rational Time Band
Authors: Anderson John R. , Qin Yulin 
Published: March 2008

Abstract
A functional magnetic resonance imaging (fMRI) study was performed in which participants performed a complex series of mental calculations that spanned about 2 min. An Adaptive Control of Thought—Rational (ACT-R) model [Anderson, J. R. How can the human mind occur in the physical universe? New York: Oxford University Press, 2007] was developed that successfully fit the distribution of latencies. This model generated predictions for the fMRI signal in six brain regions that have been associated with modules in the ACT-R theory. The model's predictions were confirmed for a fusiform region that reflects the visual module, for a prefrontal region that reflects the retrieval module, and for an anterior cingulate region that reflects the goal module. In addition, the only significant deviations to the motor region that reflects the manual module were anticipatory hand movements. In contrast, the predictions were relatively poor for a parietal region that reflects an imaginal module and for a caudate region that reflects the procedural module. Possible explanations of these poor fits are discussed. In addition, exploratory analyses were performed to find regions that might correspond to the predictions of the modules.
Infection, Incest, and Iniquity: Investigating the Neural Correlates of Disgust and Morality
Authors: Schaich Borg Jana , Lieberman Debra , Kiehl Kent A. 
Published: March 2008

Abstract
Disgust, an emotion related to avoiding harmful substances, has been linked to moral judgments in many behavioral studies. However, the fact that participants report feelings of disgust when thinking about feces and a heinous crime does not necessarily indicate that the same mechanisms mediate these reactions. Humans might instead have separate neural and physiological systems guiding aversive behaviors and judgments across different domains. The present interdisciplinary study used functional magnetic resonance imaging (n = 50) and behavioral assessment to investigate the biological homology of pathogen-related and moral disgust. We provide evidence that pathogen-related and sociomoral acts entrain many common as well as unique brain networks. We also investigated whether morality itself is composed of distinct neural and behavioral subdomains. We provide evidence that, despite their tendency to elicit similar ratings of moral wrongness, incestuous and nonsexual immoral acts entrain dramatically separate, while still overlapping, brain networks. These results (i) provide support for the view that the biological response of disgust is intimately tied to immorality, (ii) demonstrate that there are at least three separate domains of disgust, and (iii) suggest strongly that morality, like disgust, is not a unified psychological or neurological phenomenon.
The Left Posterior Superior Temporal Gyrus Participates Specifically in Accessing Lexical Phonology
Authors: Graves William W. , Grabowski Thomas J. , Mehta Sonya , Gupta Prahlad 
Published: March 2008

Abstract
Impairments in phonological processing have been associated with damage to the region of the left posterior superior temporal gyrus (pSTG), but the extent to which this area supports phonological processing, independent of semantic processing, is less clear. We used repetition priming and neural repetition suppression during functional magnetic resonance imaging (fMRI) in an auditory pseudoword repetition task as a semantics-free model of lexical (whole-word) phonological access. Across six repetitions, we observed repetition priming in terms of decreased reaction time and repetition suppression in terms of reduced neural activity. An additional analysis aimed at sublexical phonology did not show significant effects in the areas where repetition suppression was observed. To test if these areas were relevant to real word production, we performed a conjunction analysis with data from a separate fMRI experiment which manipulated word frequency (a putative index of lexical phonological access) in picture naming. The left pSTG demonstrated significant effects independently in both experiments, suggesting that this area participates specifically in accessing lexical phonology.
Inefficiency in Self-organized Attentional Switching in the Normal Aging Population is Associated with Decreased Activity in the Ventrolateral Prefrontal Cortex
Authors: Hampshire Adam , Gruszka Aleksandra , Fallon Sean J. , Owen Adrian M. 
Published: March 2008

Abstract
Studies of the aging brain have demonstrated that areas of the frontal cortex, along with their associated top-down executive control processes, are particularly prone to the neurodegenerative effects of age. Here, we investigate the effects of aging on brain and behavior using a novel task, which allows us to examine separate components of an individual's chosen strategy during routine problem solving. Our findings reveal that, contrary to previous suggestions of a specific decrease in cognitive flexibility, older participants show no increased level of perseveration to either the recently rewarded object or the recently relevant object category. In line with this lack of perseveration, lateral and medial regions of the orbito-frontal cortex, which are associated with inhibitory control and reward processing, appear to be functionally intact. Instead, a general loss of efficient problem-solving strategy is apparent with a concomitant decrease in neural activity in the ventrolateral prefrontal cortex and the posterior parietal cortex. The dorsolateral prefrontal cortex is also affected during problem solving, but age-related decline within this region appears to occur at a later stage.
Mid-fusiform Activation during Object Discrimination Reflects the Process of Differentiating Structural Descriptions
Authors: Liu Xun , Steinmetz Nicholas A. , Farley Alison B. , Smith Charles D. , Joseph Jane E. 
Published: March 2008

Abstract
The present study explored constraints on mid-fusiform activation during object discrimination. In three experiments, participants performed a matching task on simple line configurations, nameable objects, three dimensional (3-D) shapes, and colors. Significant bilateral mid-fusiform activation emerged when participants matched objects and 3-D shapes, as compared to when they matched two-dimensional (2-D) line configurations and colors, indicating that the mid-fusiform is engaged more strongly for processing structural descriptions (e.g., comparing 3-D volumetric shape) than perceptual descriptions (e.g., comparing 2-D or color information). In two of the experiments, the same mid-fusiform regions were also modulated by the degree of structural similarity between stimuli, implicating a role for the mid-fusiform in fine differentiation of similar visual object representations. Importantly, however, this process of fine differentiation occurred at the level of structural, but not perceptual, descriptions. Moreover, mid-fusiform activity was more robust when participants matched shape compared to color information using the identical stimuli, indicating that activity in the mid-fusiform gyrus is not driven by specific stimulus properties, but rather by the process of distinguishing stimuli based on shape information. Taken together, these findings further clarify the nature of object processing in the mid-fusiform gyrus. This region is engaged specifically in structural differentiation, a critical component process of object recognition and categorization.
Neural Mechanisms Underlying Adaptive Actions after Slips
Authors: Marco-Pallarés Josep , Camara Estela , Münte Thomas F. , Rodríguez-Fornells Antoni 
Published: March 2008

Abstract
An increase in cognitive control has been systematically observed in responses produced immediately after the commission of an error. Such responses show a delay in reaction time (post-error slowing) and an increase in accuracy. To characterize the neurophysiological mechanism involved in the adaptation of cognitive control, we examined oscillatory electrical brain activity by electroencephalogram and its corresponding neural network by event-related functional magnetic resonance imaging in three experiments. We identified a new oscillatory theta-beta component related to the degree of post-error slowing in the correct responses following an erroneous trial. Additionally, we found that the activity of the right dorsolateral prefrontal cortex, the right inferior frontal cortex, and the right superior frontal cortex was correlated with the degree of caution shown in the trial following the commission of an error. Given the overlap between this brain network and the regions activated by the need to inhibit motor responses in a stop-signal manipulation, we conclude that the increase in cognitive control observed after the commission of an error is implemented through the participation of an inhibitory mechanism.
Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers
Authors: Moens Maurice , Li Yu , Ou Shiqi , Liu Xuemin , Peng Deliang 
Published: March 2008

AbstractA single randomly amplified polymorphic DNA (RAPD) marker, OPA06477, species-specific to the soybean cyst nematode species, Heterodera glycines, was identified. After sequencing the RAPD-PCR products, primers of 24 nucleotides were designed to complement the terminal DNA sequence of the DNA fragments. This resulted in one pair of species-specific primers that were used to amplify the sequence characterised amplified regions (SCAR). The developed sets of SCAR primers were successfully used in straightforward, fast and reliable PCR assays to identify H. glycines. The SCAR markers can be amplified from DNA from single second-stage juveniles and females. This is the first time SCAR primers have been combined with universal primers D2A and D3B in order to avoid false negative results.
The early embryonic development of the satellite organism Pristionchus pacificus: differences and similarities with Caenorhabditis elegans
Authors: Borgonie Gaetan , Bert Wim , Houthoofd Wouter , Vangestel Sandra 
Published: March 2008

AbstractAs a comparative counterpart for the model organism Caenorhabditis elegans, the nematode Pristionchus pacificus was established as a satellite organism to study developmental processes. However, these studies mainly focused on post-embryonic development and little is known about the early embryonic development. Using 4D microscopy we reconstructed the early embryonic cell lineage of 12 individuals of P. pacificus. By analysing several parameters of early development, including the division sequence, the spatial arrangement of blastomeres, the cell cycle patterns of the AB lineage and cell-cell contacts in different cell stages of the embryo, it was shown that the early embryonic development is nearly identical to C. elegans. Known cell-cell contacts necessary for induction of blastomere fates in C. elegans are also present in P. pacificus. Thus, the spatio-temporal conditions that would allow possible homologous inductions are present. However, at least one model for blastomere specification seems not to apply to P. pacificus since the third division in the AB lineage differs from that of C. elegans. Furthermore, naturally occurring variability of early development was demonstrated, which is clearly permitted since there seems to be no influence on further development into an adult worm.
Heterorhabditis georgiana n. sp. (Rhabditida: Heterorhabditidae) from Georgia, USA
Authors: Mbata George , Shapiro-Ilan David , Nguyen Khuong 
Published: March 2008

AbstractIn a survey of entomopathogenic nematodes in Georgia, a nematode isolate of the genus Heterorhabditis was collected from soil by the insect-baiting technique and maintained in the laboratory on last instar Galleria mellonella (L.) larvae. Morphological and molecular studies of the isolate showed that the nematode is a new species. Light and scanning electron microscopy, DNA characterisation and phylogeny were used for this description. Heterorhabditis georgiana n. sp. is morphologically similar to H. bacteriophora and can be distinguished from this species mainly by male and female characters. Sixty percent of males have three pairs of papillae in the terminal group; 40% with three papillae on one side and two papillae on the other. The tail is conoid and slightly curved ventrally. The percentage of the gubernaculum to spicule length (GS%) is similar to that of H. mexicana (56) but higher than that of H. bacteriophora and species in the indica-group and the length of the spicule relative to anal body diam. (SW%) is lower than that of H. baujardi (172 vs 182), H. indica (172 vs 187) and H. bacteriophora (172 vs 174), but higher than that of H. amazonensis (172 vs 152), H. floridensis (172 vs 157) and H. mexicana (172 vs 167). The female can be differentiated from related species by its unique vulva pattern. In phylogenetic trees of ITS and D2D3 regions, the new species and H. bacteriophora form a monophyletic group. The new species has evolved five autapomorphic nucleotide character states based on analysis of ITS, differing from its sister taxon H. bacteriophora at 29 and three aligned positions of ITS and D2D3 regions, respectively.
Mussel-farming effects on Mediterranean benthic nematode communities
Authors: Mahmoudi Ezzeddine , Hedfi Amor , Essid Naceur , Beyrem Hamouda , Aïssa Patricia , Boufahja Fehmi , Vitiello Pierre 
Published: March 2008

AbstractMussel aquaculture activities in coastal areas are growing rapidly throughout the world, inducing an increasing interest and concern for their potential impact on coastal marine environments. We have investigated the impact of organic loads due to the biodeposition of a mussel farm in a lagoonar ecosystem of the Mediterranean Sea (Bizerta lagoon, northern Tunisia) on the benthic environment. The most evident changes in the benthic habitat under the farm were a strong reduction of oxygen penetration into the bottom sediments and a large accumulation of chlorophyll a (concentrations up to 16 μg g–1), phaeopigments (concentrations up to 48 μg g–1) and total organic matter (concentrations up to 12%). Results from univariate analysis of the nematofaunal data show that the nematode abundance increased in all the stations located inside the mussel farm (I1, I2, I3) and the site I2, located in the centre of the mussel farm, was the most affected. At this site, Shannon-Wiener index H′, species richness (d), evenness (J′) and number of species (S) decreased significantly. Results from multivariate analyses of the species abundance data demonstrated that responses of nematode species to the organic matter enrichment were varied: Mesacanthion diplechma was eliminated at the most affected station (I2), whereas the abundances of Paracomesoma dubium, Terschellingia longicaudata and T. communis were significantly enhanced. Responses of free-living nematodes to mussel farm biodeposition (elimination of some species and increase or decrease of some others) could lead to food limitation for their predators that, ultimately, could alter entire communities and ecosystems. Consequently, we suggest that site-specific hydrodynamic and biogeochemical conditions should be taken into account when planning new mussel farms, and meiobenthic communities should be monitored before and after farm development to prevent excessive modifications of benthic assemblage structure.
Human Influences on Wildfire in Alaska from 1988 through 2005: An Analysis of the Spatial Patterns of Human Impacts
Authors: Calef M. P. , McGuire A. D. , Chapin F. S. 
Published: March 2008

AbstractBoreal ecosystems in Alaska are responding to climate change in many ways, including changes in the fire regime. While large-scale wildfires are an essential part of the boreal forest ecosystem, humans are changing fire regimes through ignition and suppression. The authors analyzed the impact humans have on fire ignitions and relative area burned with distance into the forest from human access points such as settlements, highways, and major rivers in Alaska from 1988 to 2005. Additionally, a fire prediction model was created to identify drivers for lightning fires in the boreal forest. Human presence increases the number of ignitions near settlements, roads, and rivers and appears to reduce the area burned within 30–40 km of villages and rivers. In contrast to fires near roads and rivers, human presence may somewhat increase the area burned within 30–40 km of highways. The fire prediction model indicated that the probability of fire increases as distance from human settlements increases. In contrast, the model indicated that the probability of fire decreases as distance from roads increases and that the probability of fire in relation to distance from rivers depends on the year of analysis. While the ecological consequences of these human impacts are still unclear, this research shows that human influences on fire regime clearly affect the pattern of fire within 40 km of settlements, which is an area that represents 31% of interior Alaska. Future research should focus on more completely understanding the role of human presence in the suppression of wildfires in interior Alaska.
Climatology of the Sierra Nevada Mountain-Wave Events
Authors: Grubišić Vanda , Billings Brian J. 
Published: March 2008

Abstract
This note presents a satellite-based climatology of the Sierra Nevada mountain-wave events. The data presented were obtained by detailed visual inspection of visible satellite imagery to detect mountain lee-wave clouds based on their location, shape, and texture. Consequently, this climatology includes only mountain-wave events during which sufficient moisture was present in the incoming airstream and whose amplitude was large enough to lead to cloud formation atop mountain-wave crests. The climatology is based on data from two mountain-wave seasons in the 1999–2001 period. Mountain-wave events are classified in two types according to cloud type as lee-wave trains and single wave clouds. The frequency of occurrence of these two wave types is examined as a function of the month of occurrence (October–May) and region of formation (north, middle, south, or the entire Sierra Nevada range). Results indicate that the maximum number of mountain-wave events in the lee of the Sierra Nevada occurs in the month of April. For several months, including January and May, frequency of wave events displays substantial interannual variability. Overall, trapped lee waves appear to be more common, in particular in the lee of the northern sierra. A single wave cloud on the lee side of the mountain range was found to be a more common wave form in the southern Sierra Nevada. The average wavelength of the Sierra Nevada lee waves was found to lie between 10 and 15 km, with a minimum at 4 km and a maximum at 32 km.