bluuebunny/crossref_metadata_2025_split huggingface dataset:
B cell responses and antibody‐based therapeutic perspectives in human cancers
Authors: Mandal Gunjan , Pradhan Suchismita 
Published: March 2024

AbstractBackgroundImmuno‐oncology has been focused on T cell‐centric approaches until the field recently started appreciating the importance of tumor‐reactive antibody production by tumor‐infiltrating plasma B cells, and the necessity of developing novel therapeutic antibodies for the treatment of different cancers.Recent FindingsB lymphocytes often infiltrate solid tumors and the extent of B cell infiltration normally correlates with stronger T cell responses while generating humoral responses against malignant progression by producing tumor antigens‐reactive antibodies that bind and coat the tumor cells and promote cytotoxic effector mechanisms, reiterating the fact that the adaptive immune system works by coordinated humoral and cellular immune responses. Isotypes, magnitude, and the effector functions of antibodies produced by the B cells within the tumor environment differ among cancer types. Interestingly, apart from binding with specific tumor antigens, antibodies produced by tumor‐infiltrating B cells could bind to some non‐specific receptors, peculiarly expressed by cancer cells. Antibody‐based immunotherapies have revolutionized the modalities of cancer treatment across the world but are still limited against hematological malignancies and a few types of solid tumor cancers with a restricted number of targets, which necessitates the expansion of the field to have newer effective targeted antibody therapeutics.ConclusionHere, we discuss about recent understanding of the protective spontaneous antitumor humoral responses in human cancers, with an emphasis on the advancement and future perspectives of antibody‐based immunotherapies in cancer.
Financing sustainability: How environmental disclosures shape bank lending decisions in emerging markets
Authors: Hui Zhiyang , Li Haoyu , Elamer Ahmed A. 
Published: March 2024

AbstractAs global awareness of environmental responsibilities intensifies, the significance of corporate Environmental information disclosure (EID) in decision‐making becomes increasingly prominent. However, its influence on bank lending decisions, especially in emerging markets like China, remains debated. Using 27,095 firm‐year observations between 2008 and 2020, this study examines the impact of both voluntary and mandatory EID on bank lending decisions. Findings indicate that banks incorporate EID into their lending decisions, offering favorable bank loan terms, both in terms of size and costs to firms with strong EID. To mitigate endogeneity concerns, we employ propensity score matching and a difference‐in‐difference methodology grounded in China's newly amended Environmental Protection Law, with consistent results across both tests. Our research identifies two possible economic mechanisms to explain why EID influences bank loan features: EID's potential to reduce firm‐specific risks and its alignment with local governmental incentives for green finance. Furthermore, our research suggests that voluntary EID, previously overlooked, proves more valuable than mandatory EID. We also find that the effectiveness of EID relies on banks' evaluations of the firm's sincerity and incentives behind the disclosure. Banks show a preference for voluntary EID from firms with minimal adverse selection concerns and mandatory EID from those highly motivated to disclose voluntarily. Moreover, we identify a synergy between EID and bank loans in fostering green innovation. This research not only bridges gaps in the existing bank financing literature but also offers insights into how EID can drive sustainable economic activities in developing economies.
Influence of molecular marker type on estimating effective population size and other genetic parameters in a critically endangered parrot
Authors: Olah George , Waples Robin S. , Stojanovic Dejan 
Published: March 2024

AbstractGenetics is a fast‐moving field, and for conservation practitioners or ecologists, it can be bewildering. The choice of marker used in studies is fundamental; in the literature, preference has recently shifted from microsatellites to single nucleotide polymorphism (SNP) loci. Understanding how marker type affects estimates of population genetic parameters is important in the context of conservation, especially because the accuracy of estimates has a bearing on the actions taken to protect threatened species. We compare parameter estimates between seven microsatellites, 3761 SNP loci, and a random subset of 100 SNPs for the exact same 324 individual swift parrots, Lathamus discolor, and also use 457 additional samples from subsequent years to compare SNP estimates. Both marker types estimated a lower HO than HE. We show that microsatellites and SNPs mainly indicate a lack of spatial genetic structure, except when a priori collection locations were used on the SNP data in a discriminant analysis of principal components (DAPC). The 100‐SNP subset gave comparable results to when the full dataset was used. Estimates of effective population size (Ne) were comparable between markers when the same individuals were considered, but SNPs had narrower confidence intervals. This is reassuring because conservation assessments that rely on population genetic estimates based on a few microsatellites are unlikely to be nullified by the general shift toward SNPs in the literature. However, estimates between markers and datasets varied considerably when only adult samples were considered; hence, including samples of all age groups is recommended to be used when available. The estimated Ne was higher for the full SNP dataset (2010–2019) than the smaller comparison data (2010–2015), which might be a better reflection of the species status. The lower precision of microsatellites may not necessarily be a barrier for most conservation applications; however, SNPs will improve confidence limits, which may be useful for practitioners.
Assessing for differences in opioid administration during inpatient end‐of‐life care for patients with limited English proficiency
Authors: Curatola Nicole , Prasad Priya , Bell Brieze K. , Fang Margaret C. , Rambachan Aksharananda 
Published: March 2024

AbstractBackgroundPatients with limited English proficiency (LEP) may have worse health outcomes and differences in processes of care. Language status may particularly affect situations that depend on communication, such as symptom management or end‐of‐life (EOL) care.ObjectiveThe objective of this study was to assess whether opioid prescribing and administration differs by English proficiency (EP) status among hospitalized patients receiving EOL care.MethodsThis single‐center retrospective study identified all adult patients receiving “comfort care” on the general medicine service from January 2013 to September 2021. We assessed for differences in the quantity of opioids administered (measured by oral morphine equivalents [OME]) by patient LEP status using multivariable linear regression, controlling for other patient and medical factors.ResultsWe identified 2652 patients receiving comfort care at our institution during the time period, of whom 1813 (68%) died during the hospitalization. There were no significant differences by LEP status in terms of mean OME per day (LEP received 30.8 fewer OME compared to EP, p = .91) or in the final 24 h before discharge (LEP received 61.7 more OME compared to EP, p = .80).ConclusionLEP was not associated with differences in the amount of opioids received for patients whose EOL management involved standardized order sets for symptom management at our hospital.
Corrosion inhibition of two tetracycline‐based expired antibiotics as eco‐friendly inhibitors for mild steel in 1M HCl solution
Authors: Li Yiyao , Miao Ruirui , Li Yi , Peng Xiaolin , Niu Lin 
Published: March 2024

AbstractTwo expired Tetracycline‐based antibiotics (Oxytetracycline and Tetracycline) were utilized as eco‐friendly corrosion inhibitors for mild steel in 1M HCl solution, employing weight loss analysis, electrochemical measurements, scanning electron microscopy, and so on. The effect and mechanism were discussed using thermodynamic modeling, quantum chemical calculation, molecular dynamics simulation, and so on. The corrosion inhibition rate (η) increases with higher concentration and temperature, leading to reduced heat absorption and increased order degree. The of Oxytetracycline and Tetracycline was 96.14% and 97.92% at 323.15 K of 2 × 10−4 mol L−1, respectively. The key factors affecting η in the kinetic model parameters vary at lower concentrations. Both of them belong to cathodic corrosion inhibitors and undergo Langmuir spontaneous adsorption processes involving both physisorption and chemisorption. The disparity in energy levels between the highest occupied molecular orbital and lowest unoccupied molecular orbital, global hardness, electronegativity, molecular polarizability, dipole moment, and transfer electron number are key factors that enhance a corrosion inhibitor's ability to adsorb chloride ions.
Origin of Melt Memory Effects in Poly(ethylene oxide): The Crucial Role of Entanglements
Authors: Sangroniz Leire , Müller Alejandro J. , Cavallo Dario 
Published: March 2024

AbstractThe melt memory effect on crystallization is an intriguing phenomenon displayed by semicrystalline polymers, as opposed to low molar mass molecules. It concerns the effect of melt temperature on nucleation upon recrystallization. Typically, polymer crystals must be considerably superheated to erase the effect of previous morphology on the subsequent crystallization, avoiding an acceleration of the process. Despite being known for decades, its origin is still not fully understood. Investigating model poly(ethylene oxide) covering a wide range of molar mass, it is demonstrated that melt memory originates from topological constraints among the chains, i.e., entanglements, for PEO in which weak intermolecular interactions are present due to the ether groups. In fact, no memory is observed for samples below the critical molar mass for the formation of entanglements (about 1 kg mol−1). The increase in molar mass raises the number of entanglements and induces the formation of folded chains crystals, both factors leading to a topologically complex amorphous phase, enhancing the melt memory effect. The molecular origin of the melt memory effect in polymers with weak intermolecular interactions is thus ascribed to a slower isotropization in the melt of the chain segments originally contained in the crystals, due to the presence of entanglements among the chains. This study defines the distinction between small molecules and polymers from the point of view of melt memory.
Azobenzene‐Based Conjugated Polymers: Synthesis, Properties, and Biological Applications
Authors: Ma Zhuang , Wu Jiatao , Tan Ying , Tan Chunyan 
Published: March 2024

AbstractConjugated polymers (CPs) have been developed quickly as an emerging functional material with applications in optical and electronic devices, owing to their highly electron‐delocalized backbones and versatile side groups for facile processibility, high mechanical strength, and environmental stability. CPs exhibit multistimuli responsive behavior and fluorescence quenching properties by incorporating azobenzene functionality into their molecular structures. Over the past few decades, significant progress has been made in developing functional azobenzene‐based conjugated polymers (azo‐CPs), utilizing diverse molecular design strategies and synthetic pathways. This article comprehensively reviews the rapidly evolving research field of azo‐CPs, focusing on the structural characteristics and synthesis methods of general azo‐CPs, as well as the applications of charged azo‐CPs, specifically azobenzene‐based conjugated polyelectrolytes (azo‐CPEs). Based on their molecular structures, azo‐CPs can be broadly categorized into three primary types: linear CPs with azobenzene incorporated into the side chain, linear CPs with azobenzene integrated into the main chain, and branched CPs containing azobenzene moieties. These systems are promising for biomedical applications in biosensing, bioimaging, targeted protein degradation, and cellular apoptosis.
cGAS–STING, an important signaling pathway in diseases and their therapy
Authors: Li Qijie , Wu Ping , Du Qiujing , Hanif Ullah , Hu Hongbo , Li Ka 
Published: March 2024

AbstractSince cyclic guanosine monophosphate‐adenosine monophosphate synthase (cGAS)–stimulator of interferon genes (STING) signaling pathway was discovered in 2013, great progress has been made to elucidate the origin, function, and regulating mechanism of cGAS–STING signaling pathway in the past decade. Meanwhile, the triggering and transduction mechanisms have been continuously illuminated. cGAS–STING plays a key role in human diseases, particularly DNA‐triggered inflammatory diseases, making it a potentially effective therapeutic target for inflammation‐related diseases. Here, we aim to summarize the ancient origin of the cGAS–STING defense mechanism, as well as the triggers, transduction, and regulating mechanisms of the cGAS–STING. We will also focus on the important roles of cGAS–STING signal under pathological conditions, such as infections, cancers, autoimmune diseases, neurological diseases, and visceral inflammations, and review the progress in drug development targeting cGAS–STING signaling pathway. The main directions and potential obstacles in the regulating mechanism research and therapeutic drug development of the cGAS–STING signaling pathway for inflammatory diseases and cancers will be discussed. These research advancements expand our understanding of cGAS–STING, provide a theoretical basis for further exploration of the roles of cGAS–STING in diseases, and open up new strategies for targeting cGAS–STING as a promising therapeutic intervention in multiple diseases.
Potential Function of 3,5‐Dihydroxy‐4‐Methoxybenzyl Alcohol from Pacific Oyster (Crassostrea gigas) in Brain of Old Mice
Authors: Chen MinYu , Liu Min , Chen JingHong , Liu Xinwei , Tang LiWei , Wang Chao , Yu Ziniu , Zhang Yang , Tian Jing 
Published: March 2024

Scope3,5‐Dihydroxy‐4‐methoxybenzyl alcohol (DHMBA) is found in oyster extracts in recent years and is reported to have antioxidant activity. Although it has been reported to be protective in various models of oxidative stress, the therapeutic effect of DHMBA on neurological damage caused by aging remains to be demonstrated.Methods and ResultsThe present study investigates the potential functions of DHMBA in brain of old C57BL/6J mice and aging cell model. Administration of DHMBA improves working memory, reduces anxiety behavior, decreases the expression levels of cell cycle proteins, cycin‐dependent kinase inhibitor 1(P21) and peptidase inhibitor 16(P16) and inhibits neuronal loss in old mice. The data obtained from the aging cell model are consistent with those from the old mice. The interaction between DHMBA and Kelch‐like ECH‐associated protein 1 (Keap1) is predicted by molecular docking assay, and then it is verified by co‐immunopricipitation (CoIP) that factor red lineage 2‐related factor 2 (Nrf2)‐Keap1 protein‐protein interaction is inhibited by DHMBA. Protein levels of Nrf2 and its target genes, such as glutathione peroxidase 4(GPX4) and heme oxygenase 1 (HO‐1), are detected in old mice and aging cell model.ConclusionThis study provides new evidence that explores the antioxidant mechanism of DHMBA and implies a potential role of DHMBA on antiaging in brain.
Visual aid for pediatric virtual musculoskeletal exams: A Barbie girl in a telehealth world
Authors: Cook Alyssa K. , Papp Britney M. , Kalpakjian Claire Z. , Daunter Alecia K. 
Published: March 2024

AbstractBackgroundTelehealth is rapidly advancing, yet musculoskeletal physical examinations pose a unique challenge with limited clinically tested tools.ObjectiveTo measure whether visual aid use improves accuracy and efficiency of musculoskeletal exam maneuvers within a mock pediatric telehealth encounter.DesignRandomized controlled trial.SettingMock telehealth encounter.ParticipantsA total of 30 volunteer dyads of children aged 4–17 years old and their parent/guardian. To be eligible to participate, the parent/guardian was required to speak and read English proficiently and have access to Zoom technology on a personal electronic device.InterventionThe control group received verbal prompts to complete 12 musculoskeletal exam maneuvers. The intervention group had the addition of a Barbie (Mattel) visual aid.Main Outcome MeasuresPrimary outcomes were accuracy (number of completed maneuvers) and efficiency (total verbal prompts, total time). Secondary outcomes were ratings of comfort using telehealth technology, ease of understanding, and perceived usefulness of the visual aid. Standard multiple regression analysis was employed, with significance defined as a p value <.05.ResultsThe intervention group completed an average of 11.67 ± 0.7 maneuvers versus 11.27 ± 1.0 in the control group (p = .21), 13:31 ± 4.02 total time (mm:ss) versus 14:47 ± 4:04 (p = .05), and 4.87 ± 4.4 verbal prompts versus 8.40 ± 4.6 (p = .04), respectively. The overall model of group allocation controlling for age was not statistically significant for total maneuvers (p = .255), total time (p = .061) or total verbal prompts (p = .095). However, group allocation significantly predicted total verbal prompts (p = .032), with participants in the intervention group requiring an average of 4.1 fewer prompts. All participants in the intervention group rated the visual aid as ‘definitely helpful’.ConclusionVisual aid use improved virtual musculoskeletal exam maneuver ease and efficiency in a pediatric population.
Extending Anisotropic Interiors admitting Vanishing Complexity in Charged f (R, T) Theory
Authors: Naseer Tayyab , Sharif M. 
Published: March 2024

AbstractThis paper extends the definition of the complexity factor for a charged self‐gravitating structure in the background of gravity. For this purpose, the modified Einstein‐Maxwell field equations and the mass function in terms of interior charge are calculated corresponding to a static sphere. The Reissner‐Nordström exterior spacetime and match it with the spherical interior at the hypersurface to determine the junction conditions are adopted then. The curvature tensor is also decomposed orthogonally, resulting in several scalar functions. Only encompasses all the required parameters and fulfills the proposed criteria to be the complexity factor for the considered setup is noticed. Moreover, some constraints to minimize the degrees of freedom in the field equations are chosen. To achieve this, complexity‐free constraint with four additional conditions depending on the matter sector that lead to different models is employed. The stability of the developed models is also analyzed in the presence and absence of charge through the standard model by varying the values of the model parameter . The presence of charge in compact models corresponding to , a polytropic and a linear equation of state make them stable for specific values of is concluded.
The evaluation of cystatin protein vaccines based on the stress response of ticks triggered by low‐temperature and toxin stress in Haemaphysalis doenitzi
Authors: Zhang Song‐Bo , Gao Zhi‐Hua , Wang Yi‐Kui , Lv Wen‐Xia , Dong Ke‐Xin , Guo Fei‐Di , Wang Run‐Ying , Yang Xiao‐Long 
Published: March 2024

AbstractBACKGROUNDTicks, which are obligate blood‐feeding parasites, transmit a wide range of pathogens during their hematophagic process. Certain enzymes and macromolecules play a crucial role in inhibition of several tick physiological processes, including digestion and reproduction. In the present study, genes encoding type 2 cystatin were cloned and characterized from Haemaphysalis doenitzi, and the potential role of cystatin in tick control was further assessed.RESULTSTwo cystatin genes, HDcyst‐1 and HDcyst‐2, were successfully cloned from the tick H. doenitzi. Their open reading frames are 390 and 426 base pairs, and the number of coding amino acids are 129 and 141, respectively. In the midgut, salivary glands, Malpighian tubules and ovaries of ticks, the relative expression of HDcyst‐1 was higher in the midgut and Malpighian tubules, and HDcyst‐2 was higher in the salivary glands of H. doenitzi, respectively. Lipopolysaccharide (LPS) injection and low‐temperature stress elevated cystatin expression in ticks. Enzyme‐linked immunosorbent assay showed that both rHDcyst‐1 and rHDcyst‐2 protein vaccines increased antibody levels in immunized rabbits. A vaccination trial in rabbits infected with H. doenitzi showed that both recombinant cystatin proteins significantly reduced tick engorgement weights and egg mass weight, in particular, rHDcyst‐1 significantly prolonged tick engorgement time by 1 day and reduced egg hatching rates by 16.9%. In total, rHDcyst‐1 and rHDcyst‐2 protein vaccinations provided 64.1% and 51.8% protection to adult female ticks, respectively.CONCLUSIONThis is the first report on the immunological characterization of the cystatin protein and sequencing of the cystatin gene in H. doenitzi. Cystatin proteins are promising antigens that have the potential to be used as vaccines for infestation of H. doenitzi control. © 2024 Society of Chemical Industry.
Population longitudinal analysis of Gait Profile Score and North Star Ambulatory Assessment in children with Duchenne muscular dystrophy
Authors: Deng Jiexin , Liu Fangli , Feng Zhifen , Liu Zhigang 
Published: March 2024

AbstractDuchenne muscular dystrophy (DMD) is a rare X‐linked recessive disorder characterized by loss‐of‐function mutations in the gene encoding dystrophin. These mutations lead to progressive functional deterioration including muscle weakness, respiratory insufficiency, and musculoskeletal deformities. Three‐dimensional gait analysis (3DGA) has been used as a tool to analyze gait pathology through the quantification of altered joint kinematics, kinetics, and muscle activity patterns. Among 3DGA indices, the Gait Profile Score (GPS), has been used as a sensitive overall measure to detect clinically relevant changes in gait patterns in children with DMD. To enhance our understanding of the clinical translation of 3DGA, we report here the development of a population nonlinear mixed‐effect model that jointly describes the disease progression of the 3DGA index, GPS, and the functional endpoint, North Star Ambulatory Assessment (NSAA). The final model consists of a quadratic structure for GPS progression and a linear structure for GPS‐NSAA correlation. Our model was able to capture the improvement in function in GPS and NSAA in younger subjects, as well as the decline of function in older subjects. Furthermore, the model predicted NSAA (CFB) at 1 year reasonably well for DMD subjects ≤7 years old at baseline. The model tended to slightly underpredict the decline in NSAA after 1 year for those >7 years old at baseline, but the prediction summary statistics were well maintained within the standard deviation of observed data. Quantitative models such as this may help answer clinically relevant questions to facilitate the development of novel therapies in DMD.