bluuebunny/crossref_metadata_2025_split huggingface dataset:
39 Effectiveness of an educational program covering on-farm finishing of beef cattle for direct-to-consumer in Kentucky
Authors: VanValin Katie , Hayes Morgan , Anderson Leslie H , Bullock Darrh , Laurent Kevin 
Published: March 2024

Abstract
An educational program was developed to educate beef producers interested in marketing beef directly to the consumer. The program consisted of online webinars and two in-person sessions that were held in 6 locations throughout the state. Online webinar topics included marketing, working with the processor, food safety, forage management, confinement facilities, and environmental stewardship. In-person topics included growth and development and nutrition. The program participants were asked to complete a post-program survey at the end of the in-person session. Twenty-five survey responses were collected from the 95 in-person participants. Of the respondents, 44% indicated they were currently finishing cattle on-farm for direct markets, and the remaining participants included commercial cow-calf producers (44%), backgrounder-stocker producers (8%), and seedstock producers (4%). Participants were asked to indicate the overall level of information gained from the in-person sessions using a 5-point Likert scale (1 = not very informative; 5 = very informative), with an average response of 4.0. When asked to rate whether participating in the in-person sessions was an effective use of time using a 5-point Likert scale (1 = not very effective; 5 = very effective), the average response was 4.45. Participants were asked to rate their knowledge of various topics before and after each session using a 5-point Likert scale. Differences in pre- and post-knowledge levels were compared using the Wilcoxon signed rank test (SAS; Version 9.4; Cary, NC, USA). Participants reported increased knowledge of the length of time required to finish cattle in various production systems (P &lt; 0.001). Participants reported an increased understanding of bunk scoring systems (P = 0.004). Participants also reported an increased knowledge related to mineral nutrition of finishing cattle (P = 0.01). When asked if participants planned to implement management changes due to this educational program 83% of respondents stated that they would implement management changes on their operations. The results from this survey suggest that participants increased their knowledge related to finishing cattle nutrition and management and that this increase in knowledge should result in on-farm changes improving the efficiency of finishing cattle for direct marketing.
32 Amazing Grazing webinar and workshop series improves producers understanding of pasture ecology
Authors: Rogers Johnny R , Poore Matt H , Shaeffer April D , Weaver Andrew R , Siciliano Paul , Harmon Deidre D 
Published: March 2024

Abstract
Grassland stewards are seeking a better understanding of the complex interactions occurring in their pastures. Information is readily available to assist producers but each farm provides its own unique challenges and opportunities. A diverse scope of knowledge and skills are required to build resilient grazing systems. To address this need Amazing Grazing conducted a webinar series (April 2023) followed by on-farm workshops in July/August 2023. Webinars were held on consecutive Wednesday evenings with 237 average number of registered attendees and 78 average number of actual participants. Topics covered during the first webinar were pasture ecology basics, plant growth principles, livestock forage demand and grazing management principles. The following week covered pasture layout and design, pasture renovation techniques and pasture soil health concepts. The final evening featured topics on electric fence basics, grazing systems for horses and small ruminants and a synopsis. NC Extension Livestock Agents assisted in the sight selection and execution of workshops (n = 8) that were held on working farms. These events provide the opportunity for hands-on instruction to demonstrate the concepts covered in the webinar series. Total workshop registration was 196, with 148 evaluations completed. Participants were 72% farmers, 1% extension agents, 10% Soil and Water Conservation District Employee, 7% NRCS employee and 10% other. The total number of pasture hectares grazed by participants was 3,217 with 1,257 ha as hay. Livestock inventories for attendees were 4,323 beef cattle, 1,366 sheep, 455 goats and 51 horses. Attendees were asked about their pre- and post- workshop knowledge (1 = Very Low; 5 = Very High) of topics presented. Participants indicated a knowledge gain (average pre = 2.78 and post = 3.91) in Concepts of Pastureland Ecology, Adaptive Management, Managing Nutrient Distribution, Pasture Renovation, Temporary Electric Fence, Troubleshooting Electric Fence and Pasture Plant Identification. Workshop impact was determined by asking attendees their post workshop intentions regarding management practices (1 = No, 2 = Maybe, 3 = Yes, 4 = Already Doing). Attendees plan to spending more time walking pasture to determine plant condition, learn more about soil, plant, animal and human interactions, increase use of temporary electric fence and adopt an adaptive management style (average score = 3.91). Continued workshop participation indicates the demand for hands-on education in these areas and that the on-farm format is an effective teaching environment.
38 Assessment of retained ownership extension programming in southeastern beef cattle herds
Authors: Justice S Maggie , Mullenix M Kim , Gadberry Shane 
Published: March 2024

Abstract
Retained ownership is a marketing strategy that involves maintaining ownership of cattle through the feedyard stage. This strategy deviates from the traditional marketing time for many Southeast U.S. cow-calf producers, which is usually centered around weaning. Selected benefits of retained ownership for the producer includes capturing information on improved genetics and being able to take full advantage of health and management programs. Producers are often unaware of how calves perform further down the production line, leaving valuable information on the table as far as management styles and genetic selection goes. Extension programs such as Alabama’s Pasture to Rail and Arkansas’ Grass to Grid were developed to help producers retain ownership on beef calves and learn how their cattle perform in the feedyard phase. These programs provide opportunities for producers to receive feedback about the profitability of the cattle they are producing, learn more about the cattle industry beyond their sector, and investigate the use of other marketing strategies. Once cattle are marketed, producers receive data on their cattle such as average daily gain, estimated feed conversion, detailed carcass data, and health performance metrics. Extension educators can help producers analyze and understand how management and genetic selection affected these outcomes and changes that could be made to their operation. These programs have also been utilized in integrated Extension research. Between the years 2018 and 2023, the Alabama Pasture to Rail Program supported data collection on 667 calves through two different studies. Data collected in these studies are currently being used to update Extension recommendations on management strategies and potential of Alabama feeder calves. Feedback from facilitators and participants in these programs is often favorable for producer implementation but limitations are still faced by Extension personnel. A survey of county agents in Arkansas was recently conducted on programs such as Grass to Grid. Of the 45 agents that responded with active beef cattle programming in their county only 8% indicated that they had active participation in the Grass to Grid Program. An additional 13% of agents did indicate that they thought their county programming and producers would benefit from programming such as Grass to Grid. Limitations to the program were listed as market viability, producer buy in and turn-around results from the feedyard currently being used. Extension programming such as retained ownership programs have a place in helping producers build more sustainable programs. However, it is important to acknowledge the strengths, weaknesses and opportunities that must be addressed for producers and Extension personnel if they are to remain a function and useful tool.
81 Effect of thymol on intake and digestion in beef cattle
Authors: Fukuda Emma , Drewery Merritt L , Jessup Russell 
Published: March 2024

Abstract
Reducing enteric methanogenesis has been an area of focus and significance as methane emissions from cattle contribute to climate change and represent a loss of dietary-derived energy. Methane mitigation strategies have been widely researched, but most are limited in practical value due to associated diminishments in animal performance. One strategy, dietary supplementation of essential oils, has demonstrated variable reductions in enteric methane production in ruminants. The efficacy of using essential oils to reduce enteric methanogenesis likely varies due to inconsistent concentrations of bioactive components. Therefore, it is justified to investigate the effects of these individual bioactive components on methanogenesis and animal performance. Thymol, a terpene and main bioactive component in many essential oils, has effectively reduced methane production in vitro but in vivo data are limited. The objective of this study was to assess thymol fed at levels that correspond with effective in vitro doses on intake and digestion in beef steers consuming forage. This study was approved by the Texas State University IACUC (#8693). The project was a 4×4 Latin Square where four steers were provided one of four levels of thymol (0, 120, 240, and 480 mg thymol/kg forage intake) soaked on alfalfa cubes fed at 20% of the intake from the previous day, and ad libitum hay and water daily. Periods were 28-d with 8-d to adapt steers to treatments, 5-d to measure intake and digestion, 2-d to collect additional samples (i.e., ruminal microbiome and fat tissues), and a 14-d washout period for thymol depletion from the animal system. Diet, refusal, and fecal samples were analyzed for dry matter, organic matter, and fiber. Acid detergent insoluble ash was used to calculate total fecal production for digestibility estimates. Data were analyzed using the MIXED procedure in SAS 9.4. There was no effect of thymol dose on forage OMI (4.61 kg/d ± 0.31; P ≥ 0.48), total OMI (5.65 kg/d ± 0.38; P ≥ 0.44), or total digestible OMI (3.18 kg/d ± 0.18; P ≥ 0.26). Further, organic matter and neutral detergent fiber digestibility were not affected by treatment (56.70% ± 2.81, and 63.04% ± 2.49 respectively; P ≥ 0.14). These data indicate that thymol supplementation at doses that suppress in vitro methane production do not negatively impact intake and digestion in beef cattle. Future project activities include whole genome sequencing of rumen microbial communities to evaluate the impact of thymol on populations, with an emphasis on the methanogen archaea. These data have value for establishing a novel methane-mitigating feed additive to lower the carbon footprint of cattle production without sacrificing performance.
91 Mealworm and black soldier fly larvae as protein supplements for beef steers consuming forage
Authors: Carrasco Mikael , Drewery Merritt L 
Published: March 2024

Abstract
The global population is projected to increase, indicating there will be greater demand for animal protein to meet the associated food needs. This demand will place additional pressure on livestock systems to increase output while also minimizing natural resource inputs. Insect protein has emerged as a potential environmentally sustainable alternative to conventional protein feeds, such as soybean meal. Further, insect protein does not compete in the human food market as the products of conventional oil crops often do. Mealworm larvae (MWL) has been studied in poultry and swine as an alternative protein feed; however, there is not currently research evaluating MWL for cattle. Black soldier fly larvae (BSFL) has also received attention for its potential use in livestock feed due to its scalability and nutritional value, but research in cattle is limited. The objective of this study was to evaluate the effects of whole dried MWL and defatted BSFL as protein supplements for cattle consuming forage. These procedures were approved by IACUC at Texas State University (#7726). Five ruminally cannulated steers were utilized in a 5×5 Latin square experiment to determine how MWL and BSFL supplementation affect forage utilization. Steers consuming low-quality forage (4.2% crude protein) were provided one of five treatments each period: 1) control with no supplement (CON), 2) soybean meal (CONV), 3) BSFL, 4) MWL, and a 5) 50% BSFL and 50% MWL by-weight (MIX). Supplements were provided at 100 mg N/kg BW. Periods included 8-d for treatment adaptation, 5-d for intake and digestion measurements, and 1-d for ruminal fermentation measures. Dry matter (DM), organic matter (OM), CP, neutral detergent fiber (NDF), and acid detergent fiber (ADF) were determined for diet, ort, and feces. Protein supplementation stimulated forage OM intake (P ≤ 0.01) relative to CON. There was a significant difference in FOMI (P ≤ 0.01) between BSFL and CONV. Steers receiving CONV treatment consumed 4.71 kg FOMI/d, which was significantly greater (P ≤ 0.01) than those receiving BSFL (4.30 kg/d). There were not differences in FOMI between other treatment groups. Total digestible OM intake (TDOMI) was stimulated by protein supplementation (P ≤ 0.01) from 1.94 kg/d for CON to an average of 3.24 kg/d across protein supplements. Organic matter digestibility (OMD) and neutral detergent fiber digestibility (NDFD) were not affected by treatment (P ≥ 0.37), for an average OMD of 66.5% and NDFD of 62.7%. There was also not a treatment effect (P = 0.96) on total volatile fatty acid (VFA) concentrations, although there were some shifts in molar proportions of individual VFAs across treatments. These data indicate that MWL may be more efficacious than BSFL in stimulating forage utilization in beef cattle, but both are viable protein supplements.
78 Assessing nutrient digestibility in boer×spanish castrated male goats feeding varied levels of hempseed meal
Authors: Chaudhary Santosh , Ale Khim B , Abrahamsen Frank W , Gurung Nar K 
Published: March 2024

Abstract
Hempseed meal (HSM) has gained considerable attention as a potential alternative protein source for animal feed in the US, although its FDA approval for this purpose remains pending due to an insufficiency of comprehensive scientific data. The HSM is a byproduct of hemp oil production. Tuskegee University, a ruminant coalition group in the US is assessing this byproduct in meat goats. The present study was designed to investigate the influence of varying HSM inclusion rates on the nutrient digestibility in Boer×Spanish castrated meat goats using a 4×4 Latin square design. Four uniform mature Boer×Spanish castrated goats (14-16 months) were randomly assigned to four treatments. These goats received ad libitum access to experimental diets containing 0%, 10%, 20%, 30% HSM on an as-fed basis. Experimental diets were iso-nitrogenous (crude protein: 16%) and iso-caloric (total digestible nutrients: 64%). The experiment was conducted for 21 d in four phases where 16 d was for diet adjustment and 5 d for sample collection. Feed, feces, and urine were collected during the sample collection period and composited. The feed intake and refusal, fecal and urine outputs were monitored during the sample collection period. The feed, fecal, and urine samples were analyzed for nutrient composition. Nutrient digestibility data were analyzed in SPSS 29 and Microsoft Excel 2021. Our findings revealed no significant differences (P &gt; 0.05) in the digestibility of dry matter, crude protein, crude fat, non-fibrous carbohydrate, neutral detergent fiber, hemicellulose, and ash among experimental groups, except for acid detergent fiber, where a significant difference (P &lt; 0.05) was observed. These results shed light on the potential of hempseed meal in meat-goat diets and emphasize the importance of understanding its impact on specific nutrient digestibility for optimal dietary formulation.
96 The effect of embryo stage and biopsy on embryo viability
Authors: Wells Kenleigh N , Perkins Tommy L 
Published: March 2024

Abstract
The importance of embryo biopsy is running a 50K genomic test will be completed on the DNA biopsy, giving us the ability to collect genetic prediction data before the calf is even born. The objective of this study was to compare the viability of bovine embryos (n = 24) at different stages of embryo maturation, after microblade biopsy and embryo transfer. This process was achieved by taking a small biopsy of each embryo. The results of aspirating oocytes from packing house ovaries and in vitro fertilization (IVF), within a 48 h time period marked d 0. After a 7-d incubation period, the results consisted of 14 blastocysts and 10 early blastocysts. The final sample size, of 24 embryos, differing in stages between early blastocyst (TRT 6) and blastocyst (TRT 7). All the embryos, except for one, was a Grade 1 embryo. On d 7 the embryos were grouped by stage of embryo. Each embryo was washed, and biopsied with a microblade by a single technician. After the biopsy, the embryos were incubated for a 24 h period. Immediately after the incubation period, each embryo was analyzed for viability. On d 8, TRT 7 embryos had a 50% viability rate (n = 7), whereas TRT 6 embryos had a 70% viability rate (n = 7). Data were analyzed using GLIMMIX procedure of SAS 9.4. The viability did not differ between either treatment (P = 0.58). However, the biopsy of each embryo resulted in a significant effect on embryo viability (P &lt; 0.01). In conclusion, using embryo biopsy and the stage of the embryo may affect embryo viability.
87 Functional rumen microbiological survey of triplet-bearing Polypay ewes from parturition to weaning
Authors: Purvis Katherine G , Weinert-Nelson Jennifer R , Ely Donald G , May John , Flythe Michael D , Hamilton Tracy A , Ferrell Jessica L , Davis Brittany E 
Published: March 2024

Abstract
Ewes transitioning from late gestation to early lactation face a number of challenges including physiological stress, dietary changes, and metabolic adaptations, particularly when bearing and rearing multiple offspring. Furthermore, rumen microbial composition and function has been correlated with milk production and nutrient density in transitioning dairy cows. However, limited research has evaluated the rumen microbial community of ewes during early lactation. The objective of this study was to conduct a functional microbiological survey of the ewe rumen from parturition to weaning. We hypothesized that ewes would experience fermentative disturbances post-parturition and that the rumen microbiota, and consequently, circulating metabolites, would recover over time. Nine multiparous Polypay ewes bearing triplets were fed a lactation diet consisting of alfalfa hay and concentrate mix to meet protein and energy requirements. One lamb of each triplet set was randomly selected and placed on milk replacer 24 h after birth. The two lambs remaining on the ewe were weaned at 56 d of age. Blood and rumen samples from ewes were collected via jugular venipuncture and oral intubation, respectively, at 2, 7, and 15 d of lactation in addition to at the time of weaning. Rumen bacterial functional guilds were enumerated via serial dilution. Ruminal short-chain fatty acids (SCFA) were quantified via HPLC and serum metabolites by LC-MS. All data were analyzed using PROC Mixed with repeated measures in SAS (v. 9.4, SAS Inst. Inc., Cary, NC). Total starch-utilizing bacteria remained steady during early lactation but increased 100-fold by weaning (P = 0.03). In contrast, the starch-utilizing guild sub-population lactobacilli increased 10-fold by d 15 of lactation (P &lt; 0.01) and remained constant through weaning (P &gt; 0.05). No differences were found in the viable number of rumen cellulolytic bacteria (P = 0.37) throughout the study. Ruminal propionate concentrations increased 35% (P = 0.04) from d 2 to weaning and serum propionate (36.8 ± 4.42 µM; P = 0.08) and glucose (59.8 ± 2.97 mg dL-1; P &lt; 0.01) peaked on d 15 of lactation. While ruminal acetate did not fluctuate in early lactation, it increased 58% by weaning (P &lt; 0.01) leading to an 88% increase in serum acetate (P &lt; 0.01). Ruminal butyrate increased 49% by d 15 (P = 0.01) and this was reflected in a 7-fold increase in serum butyrate (P = 0.02). However, serum β-hydroxybutyrate did not differ from parturition through weaning (P = 0.51). Results of this experiment demonstrate that rumen bacterial functional guilds, as well as rumen and serum metabolites, were lowest two days post-parturition and increased over the course of lactation. This may suggest that ruminal fermentation is suppressed post-parturition, which could have implications for early lactation management strategies of triplet-bearing ewes.
76 An in-vitro analysis of hemp as feed for beef cattle
Authors: Fruge Greyson , Boyer Hannah , Wagner Nicole , Drewery Merritt L 
Published: March 2024

Abstract
Hemp, Cannabis sativa L., is a versatile crop that has been cultivated for centuries. Cannabis production had been outlawed in the United States due to concerns related to tetrahydrocannabinol (THC) intoxication. However, recent legislation has declassified hemp as a schedule 1 drug under regulated conditions (i.e., THC &lt; 0.3%). Thus, hemp has been reclassified as an agricultural commodity, and its production for fiber, food, and medicine has increased. Hemp cultivation results in by-products that are not utilized for human needs and have potential as livestock feed, especially for ruminants. Current regulations restrict the use of hemp and its by-products in livestock systems due to the presence of cannabinoids. Thus, research on the nutritional value of hemp for cattle is lacking. The objective of this study was to characterize the nutrient concentrations, in-vitro digestibility (IVD), and cannabinoid concentrations of different fractions of the hemp plant. Procedures involving animals were approved by IACUC at Texas State University (#8497). Four replications of hemp plants of the Cherry Soda varietal were cultivated at Texas State University. Samples were air-dried and fractionated into lower (n = 4), leaves (n = 4), stalks (n = 4), and whole plant (n = 4). Dry matter, organic matter (OM), acid detergent fiber (ADF), neutral detergent fiber (NDF), and crude protein (CP) were determined. For IVD, samples were incubated in digestion vessels containing buffers and rumen fluid at 39.5°C for 48 h and subsequently analyzed for NDF. Cannabinoid analysis was conducted via high-performance liquid chromatography (HPLC). The OM was in acceptable ranges for each hemp fraction (69-95%). The NDF and ADF were within normal ranges of conventional livestock feeds and forages for most fractions, except hemp stalk (81% NDF, 69% ADF). There was high variability in CP between fractions, with the least concentrations observed in the stalk (4%) and greatest in the flower (25%). Stalks had an IVD of 21%, whole plant of 67%, flower of 87%, and leaves of 93%. Cannabinoids were present in each fraction and especially concentrated in the flower (14%) and depleted in the stalk (0.06%). Flower had THC of 0.56%, while other fractions were less than the 0.3% legal threshold. Similarly, total cannabidiol (CBD) ranged from 0.05-11.4% with the greatest concentrations in the flower and least in the stalk. Our results indicate there is potential for hemp to be integrated into cattle production, especially the leaves. Despite adequate nutritional value, there are practical limitations with feeding hemp flower to cattle. In-vivo trials are needed to assess digestibility and profile cannabinoid metabolism in various tissues before hemp can be integrated into cattle diets.