bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apert syndrome: what prenatal radiographic findings should prompt its consideration?
Authors: Quintero‐Rivera Fabiola , Robson Caroline D. , Reiss Rosemary E. , Levine Deborah , Benson Carol , Mulliken John B. , Kimonis Virginia E. 
Published: August 2006

AbstractApert syndrome was diagnosed in a newborn with typical facial and digital features whose only detected prenatal abnormality had been agenesis of the corpus callosum. This prompted a review of the central nervous system findings in all cases of Apert syndrome treated at the Craniofacial Center Boston Children's Hospital between 1978 and 2004. Two of 30 patients with Apert syndrome had prenatal identification of mild dilatation of the lateral cerebral ventricles and complete agenesis of the corpus callosum (ACC) documented with both ultrasound and MRI. Both had the common S252W mutation of FGFR2. Though cranial and orbital malformations typical of Apert were eventually seen in these fetuses in the third‐trimester, even in retrospect, these were not detectable at mid second‐trimester, ultrasound screening for congenital malformations. Hand malformations also went undetected in the second‐trimester despite extensive imaging by experienced radiologists. We conclude that prenatal ultrasonographic identification of mild ventriculomegaly or ACC should stimulate a careful search for features of Apert syndrome and prompt follow‐up imaging to look for bony abnormalities that have later onset. Prenatal molecular testing for Apert mutations should be considered in cases of mild ventriculomegaly and ACC. Copyright © 2006 John Wiley & Sons, Ltd.
Utilization review of concomitant use of potentially interacting drugs in Thai patients using warfarin therapy
Authors: Kotirum Surachai , Chaiyakunapruk Nathorn , Jampachaisri Katechan , Wattanasombat Supharat , Rojnuckarin Ponlapat 
Published: August 2006

AbstractPurposeIn Thailand, there has been no study determining the concomitant use of medications, known to potentially interact with warfarin, in patients receiving warfarin therapy. This paper examined the frequency of which specific interacting drugs were concomitantly used in warfarin users.MethodsWe retrospectively examined the database of warfarin outpatient medical records from a regional 756‐bed hospital located in the north of Thailand. All patients receiving warfarin from 10 June 1999 to 4 August 2004 were reviewed to identify all drugs possessing interaction potential with warfarin. The potential of significant interactions were divided into high, moderate and low, according to the extent of evidence documented in textbooks and literature.ResultsAmong 1093 patients receiving warfarin therapy, 914 (84%) patients received at least one potentially interacting drug and half of them (457 patients) received at least one drug with high potential for interaction. The most frequently concomitant drug that increased INR was acetaminophen (63%, 316/457). Propylthiouracil was the most frequently concomitant drug that decreased INR response (4%, 19/457), while diclofenac was the most frequently concomitant drug that increased bleeding risk (16%, 73/457).ConclusionsAbout a half of patients receiving warfarin therapy was prescribed concomitant drug(s) that has a high potential of interactions with warfarin. These patients should be closely monitored and counselled to watch for signs and symptoms of bleeding and thrombosis to avoid adverse events associated with drug interactions. Copyright © 2006 John Wiley & Sons, Ltd.
Trends in potentially inappropriate prescribing amongst older UK primary care patients
Authors: De Wilde Stephen , Carey Iain M. , Harris Tess , Richards Nicky , Victor Christina , Hilton Sean R. , Cook Derek G. 
Published: August 2006

AbstractPurposeTo examine trends in UK primary care prescribing of medications potentially inappropriate for older people in the context of published international data.MethodsAnalysis of routinely collected anonymised computerised patient records in 131 UK general practices (approximately 162 000 registered patients annually aged ≥65 years) providing data to the DIN‐LINK database between 1994 and 2003. In each year, we identified patients prescribed drugs classified by the 2003 Beers criteria as potentially inappropriate for older people.ResultsThe level of potentially inappropriate prescribing remained steady over time: in 2003 32.2% of patients received any Beers drug, and 20.5% received a drug categorised as potentially “high risk”; percentages had been 32.9% and 21.4% respectively in 1994. In 2003, co‐proxamol (93.7/1000 patients), benzodiazepines (52.4/1000 patients) and amitriptyline (45.4/1000, mainly at low doses) were the most frequently prescribed potentially inappropriate drugs. If co‐proxamol (now being withdrawn) and low‐dose amitriptyline (appropriate for neuropathic pain) are excluded, 24.8% of patients still received a potentially inappropriate prescription in 2003.ConclusionsPrescription of potentially inappropriate medication, particularly benzodiazepines, to older people remains at a high level in the UK. Levels were higher than those seen in published data from the Netherlands, however the low rate of co‐proxamol prescribing in the Netherlands explains much, but not all, of the difference. Future international comparisons, based on more careful delineation of the criteria, may play a valuable role in pharmaco‐vigilance and can identify areas where regulation of prescribing may reduce risks to older patients. Copyright © 2006 John Wiley & Sons, Ltd.
Novel AB crosslinked polymer networks based on 1‐vinylimidazole‐ terminated polyurethane and poly(methyl methacrylate)
Authors: Venkataramani Sriram , Kannan Tharanikkarasu , Radhakrishnan Ganga 
Published: August 2006

Abstract1‐Vinylimidazole‐terminated telechelic polyurethanes were prepared from 1‐vinylimidazole and bromine‐terminated polyurethane. This vinyl‐terminated telechelic polyurethane (VTPU) and methyl methacrylate were polymerized in the presence of benzoyl peroxide to prepare novel AB crosslinked polymer networks (ABCPs). These were characterized by spectral, thermal and mechanical studies. The absence of the characteristic peak of vinyl group in infrared spectra of ABCP films confirms the occurrence of crosslinking. Static mechanical testing showed that the tensile strength of ABCP increases with increasing poly(methyl methacrylate) content. Dynamic mechanical studies revealed that ABCPs, at equal compositions of VTPU and methyl methacrylate, show good damping properties. Copyright © 2006 Society of Chemical Industry
Nanocomposite fibers of poly(ethylene terephthalate) with montmorillonite and mica: thermomechanical properties and morphology
Authors: Chang Jin‐Hae , Mun Mu Kyung 
Published: August 2006

AbstractThe thermal stabilities, mechanical properties, and morphologies of nanocomposites of poly(ethylene terephthalate) (PET) with two different organoclays are compared. Dodecyltriphenylphosphonium‐montmorillonite (C12PPh‐MMT) and dodecyltriphenylphosphonium‐mica (C12PPh‐Mica) were used as reinforcing fillers in the fabrication of PET hybrid fibers. The variations of their properties with organoclay content in the polymer matrix and draw ratio (DR) are discussed. Transmission electron microscopy micrographs show that some of the clay layers are dispersed homogeneously within the polymer matrix on the nanoscale, although some clay particles are agglomerated. It was also found that the addition of only a small amount of organoclay is enough to improve the thermal stabilities and mechanical properties of the PET hybrid fibers. Even polymers with low organoclay contents (1–5 wt%) were found to exhibit much higher strength and modulus values than pure PET. In the case of C12PPh‐MMT/PET, the values of the tensile mechanical properties of the hybrid fibers were found to decrease linearly with increases in DR from 1 to 16. However, the tensile mechanical properties of the C12PPh‐Mica hybrid fibers were found to be independent of DR. Copyright © 2006 Society of Chemical Industry
Differential binding studies applying functional protein microarrays and surface plasmon resonance
Authors: Seitz Harald , Hutschenreiter Silke , Hultschig Claus , Zeilinger Carsten , Zimmermann Bastian , Kleinjung Frank , Schuchhardt Johannes , Eickhoff Holger , Herberg Friedrich W. 
Published: August 2006

AbstractA variety of different in vivo and in vitro technologies provide comprehensive insights in protein‐protein interaction networks. Here we demonstrate a novel approach to analyze, verify and quantify putative interactions between two members of the S100 protein family and 80 recombinant proteins derived from a proteome‐wide protein expression library. Surface plasmon resonance (SPR) using Biacore technology and functional protein microarrays were used as two independent methods to study protein‐protein interactions. With this combined approach we were able to detect nine calcium‐dependent interactions between Arg‐Gly‐Ser‐(RGS)‐His6 tagged proteins derived from the library and GST‐tagged S100B and S100A6, respectively. For the protein microarray affinity‐purified proteins from the expression library were spotted onto modified glass slides and probed with the S100 proteins. SPR experiments were performed in the same setup and in a vice‐versa approach reversing analytes and ligands to determine distinct association and dissociation patterns of each positive interaction. Besides already known interaction partners, several novel binders were found independently with both detection methods, albeit analogous immobilization strategies had to be applied in both assays.
Proteomics‐based identification of autoantibodies in the sera of healthy Chinese individuals from Beijing
Authors: Li Wei‐Hua , Zhao Jie , Li Hui‐Yan , Liu Hui , Li Ai‐Ling , Wang Hong‐Xia , Wang Jie , He Kun , Liang Bing , Yu Ming , Shen Bei‐Fen , Zhang Xue‐Min 
Published: August 2006

AbstractThe identification of panels of tumor antigens that elicit an antibody response may have utility in cancer screening, diagnosis and in establishing prognosis. However, autoantibodies normally exist in sera of healthy individuals and are enormously diversified. To explore the reservoir of autoantibody in healthy population, we performed a proteomics investigation of autoantibody profiles in the sera of 36 healthy Chinese individuals from Beijing, which may provide valuable reference information to the identification of disease‐specific autoantibodies. The results showed that autoantibody profiles varied individually, but some autoantibodies were identified at a high frequency in the healthy population. The autoantibodies against α‐enolase and those against heterogeneous nuclear ribonucleoprotein L were positive in more than 50% of the sera samples. The autoantibodies identified in more than 20% of samples included those against annexin II, F‐actin capping protein beta subunit and calreticulin. Some of these autoantibodies have been previously reported to be involved in autoimmune conditions and cancers. Autoantibodies in the healthy population are important as a foundation from which disease‐specific autoantibodies can be defined. Thus our report on autoantibodies in healthy individuals may be useful as a reference for defining new autoantibody biomarkers.
Age‐related proteome analysis of the mouse brain: a 2‐DE study
Authors: Carrette Odile , Burkhard Pierre R. , Hochstrasser Denis F. , Sanchez Jean‐Charles 
Published: August 2006

Abstract2‐DE remains the most popular and versatile protein separation method among a rapidly growing array of various proteomics technologies. However, variability in sample processing, experimental design and data analyses results in a limited cross‐validation between studies performed in different laboratories. One of the goals of the Human Proteome Organization (HUPO) is to establish standards and guidelines for proteomics studies. We contributed to the HUPO Brain Proteome Project by analyzing brains from neonatal and adult mice using 2‐DE. Here we propose a standard workflow to analyze 2‐DE images and extract statistically significant differences. After differential analysis and identification by MALDI‐TOF/TOF, dihydropyrimidinase‐related proteins, brain FABP, stathmin, isocitrate dehydrogenase, γ enolase, annexin V, glutamine synthetase, creatine kinase B chain, triosephosphate dehydrogenase, and malate dehydrogenase were found differentially expressed between the two groups. The functions and potential mechanisms underlying the variation observed for these proteins are discussed.
Identification of a tyrosine‐phosphorylated CCCTC‐binding nuclear factor in capacitated mouse spermatozoa
Authors: Tang Jyh‐Bing , Chen Yee‐Hsiung 
Published: August 2006

AbstractThe molecular basis of mammalian sperm capacitation, either in vivo in the female reproductive tract, or in vitro, is poorly understood. It is well known that sperm capacitation is associated with an increase in tyrosine phosphorylation of a subset of proteins. We resolved the phosphoproteins in the cell lysate of mouse sperm after capacitation by 2‐DE. One tyrosine‐phosphorylated 130‐kDa spot was trypsin‐digested, and six oligopeptide sequences were established from the MS data. These were confirmed in a CCCTC‐binding nuclear factor (CTCF), a widely expressed and highly conserved protein. Further, both an anti‐phosphotyrosine antibody and an anti‐CTCF antibody showed immunoreactivity to a 130‐kDa component in the immunoprecipitates obtained after incubation of the cell lysate from the capacitated sperm using another anti‐CTCF antibody. The data support the presence of a tyrosine‐phosphorylated CTCF in the capacitated sperm. Immunolocalization of the CTCF revealed fluorescent staining in the acrosome region in both capacitated and incapacitated sperm. The electrophoretic mobility shift assay, using a CTCF target sequence 5'‐GGCGGCGCCGCTAGGGGTCTCTCT‐3' found in the promoter of the amyloid β‐protein precursor, manifested that, relative to CTCF in the incapacitated sperm, the tyrosine‐phosphorylated protein in the capacitated sperm had stronger affinity to the CTCF target sequence.
The power of cooperative investigation: Summary and comparison of the HUPO Brain Proteome Project pilot study results
Authors: Reidegeld Kai A. , Müller Michael , Stephan Christian , Blüggel Martin , Hamacher Michael , Martens Lennart , Körting Gerhard , Chamrad Daniel C. , Parkinson David , Apweiler Rolf , Meyer Helmut E. , Marcus Katrin 
Published: August 2006

AbstractWithin the pilot phase of the HUPO Brain Proteome Project, nine participating laboratories analysed human (epilepsy and/or post mortem material) and mouse brain samples (embryonic, juvenile and adult), respectively, using a variety of different state of the art techniques. Thirty‐seven different analytical approaches were accomplished. Of these analyses, 17 were done differentially, i.e. the protein expression patterns of the different samples (human or mouse) were compared. A catalogue of all proteins present in the respective sample was built in 20 analyses (mapping). All data were collected in the Data Collection Center in Bochum, Germany, and were reprocessed according to thoroughly defined parameters. In this report, a summary of all results and inter‐laboratory comparisons with respect to the number of identified proteins, the analysed organism, and the used techniques is presented.
Screening for psychological distress in Turkish cancer patients
Authors: Özalp Elvan , Cankurtaran Eylem Sahin , Soygür Haldun , Özdemir Geyik Pinar , Jacobsen Paul B. 
Published: August 2006

AbstractThe distress thermometer (DT) is a useful measure of psychological distress in cancer patients. Our objective was to investigate distress impact on oncology patients in Turkey and determine the optimal cut‐off score on the DT for identifying clinically significant distress. One hundred and eighty two cancer patients completed the DT, Problem List (PL), and Hospital Anxiety and Depression scale (HADS), The European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ‐C30 version 3.0). The receiver operating characteristic (ROC) curve analyses of DT scores yielded an estimated area under the curve of 0.66 when compared to the HADS cut‐off score, suggesting the DT is an effective scale to discriminate between classified cancer patients both with and without clinically significant distress. The DT cut‐off score of 4 yielded the optimal combination of sensitivity and specificity. Scores on the DT were moderately correlated to the HADS (p<0.01) and EORTC QLQ‐C30. Based on the significant correlations, we conclude that the DT has acceptable criterion validity. Copyright © 2006 John Wiley & Sons, Ltd.
Standardized audiotape versus recorded consultation to enhance informed consent to a clinical trial in breast oncology
Authors: Hack Thomas F. , Whelan Tim , Olivotto Ivo A. , Weir Lorna , Bultz Barry D. , Magwood Bryan , Ashbury Fred , Brady Jane 
Published: August 2006

AbstractBackground: The purpose of this study was to systematically compare two audiotape formats for the delivery of information relevant to informed consent to participate in a clinical trial in breast oncology, and to establish the feasibility of adding a consultation recording protocol to a clinical treatment trial.Method: Participants were 69 women with newly diagnosed breast cancer and 21 oncologists from 5 Canadian cancer centers. Patients were block randomized to one of three groups: 1. standardized audiotape; 2. consultation audiotape; or 3. both audiotapes. Patients received their tapes immediately following the clinical trial consultation. Patient outcomes included perception of being informed about clinical trials, knowledge of information relevant to providing informed consent to a clinical trial, and satisfaction with communication during the consultation.Results: The consultation audiotapes contained less trial‐related information than the standardized audiotape but there were no differences in clinical trial knowledge or perception of being informed across the intervention groups. Patients expressed a marginally significant preference for consultation audiotapes over standardized audiotapes.Conclusions: Patients tended to prefer receiving an audiotape of their own consultation over a standardized audiotape. The majority of oncologists considered the audiotape intervention feasible but were less enthusiastic about being involved in a larger study given the accrual challenges that arose when trying to ‘piggy‐back’ one randomized controlled trial on an existing clinical trial. Copyright © 2006 John Wiley & Sons, Ltd.
Plasma Polymerization on Hydroxyapatite Powders to Increase Water Dispersability for Biomedical Applications
Authors: Garreta Elena , Tricás Núria , Quintana Lluis , Semino Carlos E. , Borrós Salvador 
Published: August 2006

AbstractSummary: The modification of hydroxyapatite powder using cold plasma is described. This modification can be used to improve hydroxyapatite water dispersability and to control calcium release. A downstream and a fluidized bed reactor have been successfully set‐up to perform the surface treatment on hydroxyapatite. The advantages and drawbacks of both systems are presented.Schematic of the downstream reactor sued here and an optical microscope picture of spherical HA particles.magnified imageSchematic of the downstream reactor sued here and an optical microscope picture of spherical HA particles.
Criteria for downhill protein folding: Calorimetry, chevron plot, kinetic relaxation, and single‐molecule radius of gyration in chain models with subdued degrees of cooperativity
Authors: Knott Michael , Chan Hue Sun 
Published: August 2006

AbstractRecent investigations of possible downhill folding of small proteins such as BBL have focused on the thermodynamics of non‐two‐state, “barrierless” folding/denaturation transitions. Downhill folding is noncooperative and thermodynamically “one‐state,” a phenomenon underpinned by a unimodal conformational distribution over chain properties such as enthalpy, hydrophobic exposure, and conformational dimension. In contrast, corresponding distributions for cooperative two‐state folding are bimodal with well‐separated population peaks. Using simplified atomic modeling of a three‐helix bundle—in a scheme that accounts for hydrophobic interactions and hydrogen bonding—and coarse‐grained Cα models of four real proteins with various degrees of cooperativity, we evaluate the effectiveness of several observables at defining the underlying distribution. Bimodal distributions generally lead to sharper transitions, with a higher heat capacity peak at the transition midpoint, compared with unimodal distributions. However, the observation of a sigmoidal transition is not a reliable criterion for two‐state behavior, and the heat capacity baselines, used to determine the van't Hoff and calorimetric enthalpies of the transition, can introduce ambiguity. Interestingly we find that, if the distribution of the single‐molecule radius of gyration were available, it would permit discrimination between unimodal and bimodal underlying distributions. We investigate kinetic implications of thermodynamic noncooperativity using Langevin dynamics. Despite substantial chevron rollovers, the relaxation of the models considered is essentially single‐exponential over an extended range of native stabilities. Consistent with experiments, significant deviations from single‐exponential behavior occur only under strongly folding conditions. Proteins 2006. © 2006 Wiley‐Liss, Inc.
Unambiguous prediction of human integrin transmembrane heterodimer interactions using only homologous sequences
Authors: Lin Xin , Tan Suet Mien , Law S. K. Alex , Torres Jaume 
Published: August 2006

AbstractPart of the interaction between the α‐ and β‐subunits of integrins is known to take place at the transmembrane (TM) domain, where both heteromeric and homomeric aggregates have been reported in vivo and in vitro. In a recent computational study, totally independent from biochemical or biophysical data, we explored the plausibility of various TM homo‐oligomers using evolutionary conservation data as a filter for non‐native interactions. We showed that several homodimeric and homotrimeric interactions for α‐ and β‐chains are evolutionarily conserved. We report herein the results of the application of the same exhaustive approach to the integrin heterodimer. We have studied all known human TM integrin αβ pairs, and we show unambiguously that two models of interaction are evolutionarily conserved. These two models are consistent with those proposed previously based on mutagenesis and crosslinking. Comparison with previous experimental data strongly supports that a glycophorin A‐like model is an intermediate form of interaction between the resting state and the active form, where chain separation occurs. Surprisingly, these two models are also conserved when considering most of the possible αβ pair combinations, suggesting that specific pairing of integrins is not determined by the TM domain, which has remained unchanged in spite of the variety of known integrin functions. This fact highlights a common ancestral mechanism for signal transduction that has remained through evolution. In a broader context, our results show that it is possible to obtain correct and detailed interactions of α‐helical heterodimers with total independence of experimental data. Proteins 2006. © 2006 Wiley‐Liss, Inc.