bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of the in vivo uptake and metabolism of 3H‐oestrone and 3H‐oestradiol by normal breast and breast tumour tissues in post‐menopausal women
Authors: McNeill J. M. , Reed M. J. , Beranek P. A. , Bonney R. C. , Ghilchik M. W. , Robinson D. J. , James V. H. T. 
Published: February 2007

AbstractAfter infusion of 3H‐oestradiol or 3H‐oestrone into post‐menopausal women with breast cancer, there was a significant uptake of both steroids by breast tumour and normal tissue. The proportion of oestrogen present in tumour tissue as 3H‐oestradiol after infusion of 3H‐oestradiol (89.4 ± 3.5%, mean ± SD, n = 4) was significantly higher (p < 0.001) than in normal breast tissue (72.8 ± 3.3%) obtained from the same women. Similarly, after infusion of 3H‐oestrone, the proportion of oestrogen present as 3H‐oestradiol (48.4 ± 14.4%) was significantly higher than in normal breast tissue (19.1 ± 6.4%). These results suggest that conversion of oestrone to oestradiol is enhanced in breast tumour tissue with little metabolism of oestradiol. This would account for the higher concentrations of oestradiol reported in breast tumour tissue in the presence of increased oestradiol 17β‐hydroxysteroid dehydrogenase activity.
Combined effect of ACNU and 5‐FU on rat glioma cells in spheroids and monolayer cultures
Authors: Kitahara Masakazu , Katakura Ryuichi , Mori Teruaki , Suzuki Jiro , Sasaki Takehito 
Published: February 2007

AbstractCombined effects of ACNU and 5‐Fu on rat glioma clone‐6 cells grown exponentially as monolayers and in multicell spheroids (500–600 μm in diameter) were analyzed by the colony‐forming assay. Cells in spheroids with central necroses were more sensitive to ACNU than cells in monolayer, although a large fraction of the cells in these spheroids was resistant to 5‐Fu. The effect of ACNU was enhanced by the combined treatment with 5‐Fu for cells in both spheroids and monolayers, and was more remarkable when 5‐Fu was administered 24 hr prior to ACNU treatment. The enhancement was mainly due to a decrease in the number of spheroid‐cells resistant to 5‐Fu. Isobolo‐gram analysis indicated that the enhancement was supra‐additive when a low concentration of 5‐Fu was combined with a high concentration of ACNU. It is suggested that a low dose of 5‐Fu combined with a high dose of ACNU may preferentially kill more cells in solid tumors.
Anti‐tumor effects of interferon in mice injected with interferon‐sensitive and interferon‐resistant friend leukemia cells. IV. Definition of optimal treatment regimens
Authors: Gresser Ion , Belardelli Filippo , Maury Chantal , Tovey Michael G. , Maunoury Marie‐Thérèse 
Published: February 2007

AbstractMouse interferon α/β exerted a similar anti‐tumor effect in DBA/2 mice injected i.p. with Friend eryth‐roleukemia cells (FLC) either sensitive or resistant to interferon as determined by bothin vitroandin vivoassays. Using this tumor system we attempted to define optimal treatment regimens for interferon administration. Interferon was most effective when injected at the site of tumor inoculation rather than at a distant site. Two factors seemed of especial importance: the amount of interferon injected and the frequency of interferon administration. Thus, for daily administration of interferon, the antitumor effect was directly related to the amount of interferon injected. For a given total dose of interferon, repeated administration of small doses of interferon was more effective than administration of a larger dose at more widely spaced intervals. The anti‐tumor efficacy of interferon was independent of the number of FLC inoculated when 102to 105FLC were injected, but interferon treatment was less effective when 106or 107FLC were injected. The relevance of these results to the use of interferon in patients with cancer is discussed.
A verion‐like particle (VLP) released from an epstein‐barr virus‐producing cell sub‐line of P3HR‐1: Identification of a specific cellular DNA contained within VLPs
Authors: Yano Shoki , Miyoshi Isao , Ohtsuki Yuji , Koizumi Shigeki , Osato Toyoro 
Published: February 2007

AbstractA vision‐like particle (VLP) was isolated from the culture fluid of P3HR‐1 cells. VLPs were separable from the enveloped Epstein‐Barr virus (EBV) through rate‐velocity sedimentation by virtue of their greater density. VLPs comprised electron‐dense nucleotide material and the surrounding round or oval envelope which ranged from 100 to 150 nm in diameter. The polypeptide constitution of VLPs was very similar to that of enveloped EBV by SDS‐polyacrylamide gel electrophoresis. VLPs contained reasonably specific cellular DNA (VLP DNA) with a sedimentation coefficient of 48S, which was cleaved into 5, 8, and at least 30 specific DNA fragments by digestion with BamHI, Sal, and EcoRI, respectively. Blot hybridization data suggested a limited heterogeneous genomic organization of VLP DNA and the presence of a VLP DNA‐related molecule(s) in human cord lymphocytes.
Leukemia serum reactive with retrovirus‐related antigen in normal human placenta
Authors: Maeda Shuichi , Yonezawa Kazuhiko , Yoshizaki Haruyasu , Mori Masamitu , Kobayashi Takeaki , Akahonai Yoshikazu , Yachi Akira , Mellors Robert C. 
Published: February 2007

AbstractSerum antibody reactive with a retrovirus‐related p30 antigen in human normal term placenta was investigated and characterized by immunohistologic and immunoblotting methods. Sera obtained from patients with acute leukemia and malignant lymphoma were used as first antibody, and cryostat sections of placenta were the target antigen. An IgM antibody that reacted mainly with the basal aspect of syncytio‐ trophoblast of chorionic villi, where a putative human endogenous retrovirus p30 antigen is located, was directed by indirect immunofluorescence. This antibody activity, termed anti‐basal aspect of syncytiotropho‐ blast (anti‐BAST), was detected in the sera of many patients with acute leukemia (AML, ALL) and malignant lymphoma, and less frequently in sera of pregnant women and normal controls. As shown by immunoblotting analysis, the main reactive antigen recognized by anti‐BAST was a non‐glycosylated 32‐ kDa placental protein which was antigenically related to SSAV p30. A non‐glycosylated 19‐kDa protein was also considered to be one of the anti‐BAST‐corresponding antigens. This suggests the presence of a new antigen‐antibody system of human retrovirus(es) revealed by subinfectious antigenic expression and by specific antibody activity in conditions of human health and disease, particularly, acute leukemias and malignant lymphomas of common types.
CO‐reversion of a lectin‐resistant mutation and non‐metastatic phenotype in murine tumor cells
Authors: Dennis J. W. , Laferte S. 
Published: February 2007

AbstractThree independent isolates, one obtained spontaneously and the others obtained after in vitro mutagenesis, of a WGA‐resistant mutation were compared to the highly metastatic parental murine cell line MDAY‐D2 for alterations in plasma membrane glycoproteins and changes in metastatic behavior. The mutants were non‐metastatic from an s.c. site of injection and poor organ‐colonizers when administered i.v. Each of the mutant lines had the same lesion in N‐linked oligosaccharide structure, which rendered the cells hypersensitive to the N‐acetylglucosamine‐binding lectin BSII in vitro. The phenotypic similarities between the 3 WGA‐resistant isolates indicated that the mutation was directly related to the attenuated malignant phenotype. Direct confirmation was obtained by the isolation of a BSII‐resistant clone of the mutant cells that co‐reverted for lectin‐sensitivity, lectin binding glycoproteins and malignant aggressiveness. The results indicate a direct relationship between malignant behavior and cell‐surface oligosaccharide structure.
A monoclonal antibody reacting with endothelial cells of budding vessels in tumors and inflammatory tissues, and non‐reactive with normal adult tissues
Authors: Hagemeier Hans‐Hermann , Vollmer Ekkehard , Goerdt Sergij , Schulze‐Osthoff Klaus , Sorg Clemens 
Published: February 2007

AbstractThis report describes a monoclonal antibody (MAb) generated by immunization of mice with cell suspensions of capillary‐rich fragments of mammary carcinomas. The antibody (EN 7/44) belongs to the IgM class and detects a 30.5 kDa antigen which is found in the cytoplasm of human placental and umbilical veinendothelial cells (HUVEC) and on the surface of tumor endothelium. Using indirect immunofluorescence and immunoperoxidase techniques, we did not find the MAb in peripheral blood cells or in any human cell lines tested, nor in endothelial cells from normal, non‐proliferating adult tissues. Positively staining endothelium was found in the placenta, the umbilical vein and in proliferating normal tissues (intestine). Positive endothelial cells were found in acute inflammatory reactions and in tumors. In the latter, the strongest reactions were seen in newly formed budding capillaries, which were identified by their reactivity with Ulex europaeus I‐lectin and antibodies against F VIM‐RAG. In tumor tissues, large vessels could be positively stained with the EN 7/44 antigen, in contrast to inflammatory tissues. It is concluded that endothelial cells at the tip of a budding capillary express distinct phenotypic characteristics.
A case‐control study of the efficacy of a non‐randomized breast cancer screening program in florence (Italy)
Authors: Palli Domenico , del Turco Marco Rosselli , Buiatti Eva , Carli Simona , Ciatto Stefano , Toscani Lucia , Maltoni Giancarlo 
Published: February 2007

AbstractIn a rural area near Florence a population‐based screening program for breast cancer was started in 1970, offering a mammography test every 2.5 years to all women between 40 and 70 years of age. In order to evaluate the efficacy of this program a case‐control study has been carried out comparing the screening history of all women who died from breast cancer in this area in the years 1977–1984, and who had been diagnosed after the start of the program, with that of a matched group of living controls. The case‐control study showed that the overall O.R. of dying from breast cancer in the study period for “screened” versus “never‐screened” women was 0.53 (95% C.I. = 0.29–0.95). After evaluation of other variables as potential confounders the adjusted O.R. estimates were respectively 0.57 (95% C.I. = 0.35–0.92) and 0.32 (95% C.I. = 0.20–0.52) for women screened only once or at least twice. No significant protective effect was shown for women below the age of 50 years.
Phenotypic profile of clones from early cultures of human metastatic melanomas and its modulation by recombinant interferon γ
Authors: Anichini Andrea , Mortarini Roberta , Fossati Giuseppe , Parmiani Giorgio 
Published: February 2007

AbstractFifty‐six tumor clones isolated by cloning in soft agar from early cultures (before the 10th in vitro passage) of two different human metastatic melanomas (Me9229 and Me28) were characterized by FACS analysis for surface expression of class‐I and class‐II HLA antigens and of melanoma‐associated antigens (MAA) with a panel of 15 monoclonal antibodies (MAbs). A marked phenotypic heterogeneity involving MAA and/or HLA markers was observed among the clones derived from both tumors. The differences among the tumor clones and between them and the uncloned melanoma were qualitative and quantitative for each antigen considered. Clones derived from Me9229 expressed the same HLA profile as the parental culture (class I +, class II‐) while strong heterogeneity was observed for MAA expression. Clones from Me28 presented a marked heterogeneity for class‐I and class‐II HLA antigens but were more homogeneous for MAA. The phenotype of the clones was repeatedly checked over the first month in culture and found to remain generally unchanged and not linked to the cell cycle. However, major changes in antigenic expression of the clones could be observed upon treatment with recombinant interferon‐γ (rIFN‐γ): class‐I and‐II HLA antigens could be induced or augmented while a moderate inhibition was seen on MAA expression. Furthermore, an apparent hierarchy in expression and/or induction of class‐II antigens by rIFN‐γ was observed among the tumor clones. DR antigens were more frequently expressed (Me28 clones) and upon treatment with HFN‐γ reached higher levels than DP and DQ products. Taken together these results indicate that antigenic heterogeneity for MAA and HLA antigens can be detected in cells isolated from early cultures of human metastatic melanomas and suggest that the original uncloned tumor might be considered as a complex mixed population made up of a number of neoplastic cells each expressing a distinct phenotype which can be modulated by lymphokines such as IFN‐γ.
Two new cell lines from B‐prolymphocytic leukaemia: Characterization by morphology, immunological markers, karyotype and Ig gene rearrangement
Authors: Melo Junia V. , Brito‐Babapulle Vasantha , Foroni Letizia , Robinson Daniele S. F. , Luzzatto Lucio , Catovsky Daniel 
Published: February 2007

AbstractLeukaemic cells from 2 patients with B‐prolymphocytic leukaemia were immortalized in vitro by means of Epstein‐Barr virus and phorbol‐ester TPA. The resulting cell lines, named JVM‐2 and JVM‐3, have been growing continuously in liquid culture for more than one year. JVM‐2 is characterized cytogenetically by t(11;14)(q13;q32), and JVM‐3 by trisomy 12. The immunoglobulin (Ig) heavy‐and light‐chain genes showed the same pattern of rearrangement in both lines as in the original prolymphocytes from each case. The cells from these lines showed a spectrum of morphological and immunological features corresponding to different stages of B‐cell maturation. The expression of Ig, IgM‐X in JVM‐2 and IgMD‐K in JVM‐3, changed from a predominantly membrane pattern in the original cells to a cytoplasmic one in the cell lines. By comparison with their original progenitors, the cells from both lines showed reduced reactivity with the monoclonal antibody (MAb) FMC7, and increased expression of the antigens recognized by the MAbs OKT10, α‐Tac, FMC53 and Ki‐I. The availability of cell lines from this rare type of lymphoid leukaemia offers a potential tool for the study of molecular events associated with the expression of Ig and other antigens by ncoplastic cells.
Seroepidemiologic survey of captive old‐world primates for antibodies to human and simian retroviruses, and isolation of a lentivirus from sooty mangabeys (Cercocebus atys)
Authors: Lowenstine Linda J. , Pedersen Niels C. , Higgins Joanne , Palus Katherine C. , Uyeda Ann , Marx Preston , Lerche Nicholas W. , Munn Robert J. , Gardner Murray B. 
Published: February 2007

AbstractSera from 526 Old‐World monkeys and apes, representing 50 species and 20 genera and living in US zoos and vivaria, were screened for antibodies to HTLV‐I, HTLV‐III/LAV, and simian‐AIDS retrovirus, type I (SRV‐I). Sera were screened initially by ELISA, and ELISA‐positive sera, as well as ELISA‐negative sera from cage contacts, were further tested by Western blotting. A large number of false‐positive and a small number of false‐negative ELISA sera were identified. Although most true positive reactions were directed to a single retrovirus, a number of individuals from 4 species were positive for more than one retrovirus. Specific seroreactivity to HTLV‐1 was found in 39/526 (7%) animals of 15 species. True positive reactions to SRV‐1 were found in 21/516 (4%) animals, including talapoins and 2 species of macaques. Specific serologic reactions to HTLV‐III/LAV were detected in 23/526 (4%) monkeys. Many of the HTLV‐III/LAV seropositive animals were from one mixed‐species zoo exhibit, containing sooty mangabeys, mandrills, Kolb's guenons, and talapoins. A type D virus was isolated from the blood of 3/10 SRV‐1 antibody‐positive Tonkeana macaques, but from none of 11 seropositive talapoins. A lentivirus was isolated from the blood of 4/7 HTLV‐III/LAV seropositive sooty mangabeys, but not from seropositive talapoins in the same exhibit or from 2 seropositive colobus from another zoo. The sooty mangabey lentivirus produced generalized lymphadenopathy, leukopenia, and decreased levels of T4 lymphocytes in 2 experimentally infected rhesus macaques.
Tumorigenicity of T lymphoma/T lymphoma hybrids and T lymphoma/normal cell hybrids
Authors: Hays Esther F. , Weinroth Stephen E. , Macleod Carol L. , Kitada Shinichi 
Published: February 2007

AbstractStable hybrids formed between clones of established murine T‐cell lymphoma lines, and between lymphoma clones and normal spleen or thymus cells were examined for their tumorigenic properties by intravenous (i.v.) and intradermal (i.d.) inoculation into syngeneic AKR mice. Fusion parents consisted of T lymphoma clones of high and low tumorigenicity derived from the SL 12 cell line. In addition, normal spleen cells and thymocytes were fused with poorly tumorigenic T‐lymphoma clones. Hybrids tested by i.v. inoculation of 106 cells to syngeneic hosts showed that fusion between the lymphoma cells resulted in hybrids which displayed the phenotype of the highly tumorigenic parent. Also, it was shown that fusion of poorly tumorigenic lymphoma cells with normal spleen cells resulted in hybrids with enhanced tumorigenicity. Fusion of poorly tumorigenic lymphoma cells with normal thymocytes resulted in hybrids with the highest tumorigenic potential. The pattern of spread for the tumor/tumor hybrid was that of the highly tumorigenic parent. Tumor spread patterns for the spleen/tumor hybrids were different from those of the thymocyte/tumor hybrids. Intradermal inoculation of 105 cells from tumor/spleen or tumor/thymocyte hybrids revealed differences in latent periods between parental and hybrid cells, the tumor/thymocyte hybrids having the shortest latent period. Surface marker studies and T‐cell antigen receptor mRNA determinations in the tumor cell/normal cell hybrids indicated that the normal parent was a cell of immature phenotype. Therefore, high tumorigenicity is a dominant characteristic, and poorly tumorigenic but “immortal” T lymphoma cells can derive characteristics which increase their In vivo growth capacity from the putative immature normal cells with which they selectively fuse.
Hras1‐selected, chromosome‐mediated transformants vary in phenotype in vitro and tumorigenic potential in vivo
Authors: Porteous D. J. , Morten J. E. N. , Foster M. E. , Cranston G. , Weir‐Thompson E. , Busuttil A. , Bostock C. J. , Steel C. M. 
Published: February 2007

AbstractTransfection of mouse C127 cells with mitotic chromosomes isolated from a human EJ bladder carcinoma cell line gave rise, at high frequency, to foci of transformed cells. Independent, HRAS 1‐selected chromosome‐mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony‐forming abilities in low‐melting‐point agarose. Subcutaneous inoculation of neonatally thymec‐tomized, Ara‐C‐protected, total‐body‐irradiated CBA mice was used to compare the tumorigenic potential of each transformant. Significant quantitative and qualitative differences in tumorigenicity were found between transformants which correlated with differences in malignant phenotype observed in vitro. The sensitivity of the tumorigenicity assay is such that rare transformation events can be selected directly in vivo.
Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 72) on a novel pancarcinoma antigen (TAG‐72)
Authors: Klug Thomas L. , Sattler Marcia A. , Colcher David , Schlom Jeffrey 
Published: February 2007

AbstractCA 72 is a monoclonal antibody (MAb) ‐defined antigenic determinant expressed on a pancarcinoma antigen (TAG‐72) found in more than 85% of human colorectal carcinomas. An immunoradiometric assay has been developed using the murine MAb B72.3 to quantitate CA 72 in human serum. In a simultaneous immunoradiometric assay, the mean CA 72 concentration in 1,099 serum samples from healthy blood donors was 1.83±2.03 (SD) units/ml. If the upper limit of normal was set at 10 u/ml of serum, a value including 99% of healthy blood donors, only 4 of 101 serum samples (4%) from patients with benign disease were elevated, whereas 15 of 26 (58%) and 14 of 25 (56%) of rectal and colon carcinoma patient sera, respectively, were positive. Serum samples from 84 benign colorectal disease cases were examined; of these, 0 of 28 (0%) colorectal adenoma, 1 of 39 (3%) ulcerative proctocolitis, 0 of 15 (0%) diverticulosis, and 0 of 2 (0%) irritable bowel disease sera contained more than 10 u/ml CA 72. At a reference value of 20 u/ml, 0 of 101 (0%) benign disease and 2 of 1,060 (0.2%) blood donor sera had elevated values, whereas 10 of 26 (38%) and 9 of 25 (36%) rectal and colon patient sera, respectively, remained positive. The majority of patients with pancreatic and ovarian cancer, and a significant fraction of stomach cancer patient sera, also contained elevated levels of CA 72. The ability of this assay to discriminate between malignant and benign diseases suggests its further evaluation for monitoring and diagnosis in groups at risk for development of cancer.
Rearranged HPV 16 molecules in an anal and in a laryngeal carcinoma
Authors: Scheurlen Wolfram , Stremlau Armin , Gissmann Lutz , Höhn Dieter , Zenner Hans‐Peter , Hausen Harald Zur 
Published: February 2007

AbstractBy hybridization under stringent conditions, one out of two anal carcinomas and one out of 36 laryngeal carcinomas were shown to harbor HPV 16 DNA in high copy number. Further analysis of both tumor DNAs indicated a rearrangement of the viral DNA in the tumor cells. HPV 16 DNA in the anal carcinoma could chiefly be found episomally in two different forms: (1) a minority as 7.9‐kb oligomeric episomes with no apparent modifications; (2) as 10.7‐kb rearranged oligomeric episomes with a duplication of the part of the viral genome encoding the open reading frames (ORF) E7, E1 and parts of E6 and E2. In the laryngeal carcinoma, integrated and episomal HPV 16 DNA molecules of 7.9 kb were present, together with rearranged molecules of approximately 18 kb with multiple duplications of the ORF E4 and parts of the ORFs E2, E5, L1 and L2. Possible consequences for transcription of the modified viral genomes are discussed.
Tumour‐promoting effect of chilli extract in BALB/c mice
Authors: Agrawal R. C. , Wiessler M. , Hecker E. , Bhide S. V. 
Published: February 2007

AbstractIn 50% of BALB/c mice pretreated with atropine, tongue tumours were induced by fortnightly application of DMN‐OAc (2 mg/kg) on the tongue. When DMN‐OAc + TPA was used for the initiation‐promotion protocol, tumours were observed on the tongue, the site of application, in only 10% of animals. In the same group, stomach tumours were obtained in 63% of mice, denoting that initiation‐promotion could be successfully used to induce stomach tumours. Using a protocol of DMN‐OAc + chilli as a promoter, we observed induction of stomach tumours. The promoter effect of chilli extract was also seen in the BHC‐induced hepato‐carcinogenesis system. It thus appears that, in BALB/c mice, chilli acts as a promoter in stomach and liver carcinogenesis.