bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chiral Macrocyclic Aliphatic Oligoimines Derived from trans‐1,2‐Diaminocyclohexane
Authors: Kwit Marcin , Plutecka Agnieszka , Rychlewska Urszula , Gawroński Jacek , Khlebnikov Alexander F. , Kozhushkov Sergei I. , Rauch Karsten , de Meijere Armin 
Published: July 2007

AbstractAliphatic dialdehydes of rigid structures having a cyclohexane, a bicyclo[2.2.2]octane or a [7]triangulane skeleton, have been condensed with enantiomerically pure trans‐1,2‐diaminocyclohexane to give [3+3] or [2+2] macrocyclization products. Unlike acyclic aliphatic imines, these macrocyclic oligoimines show enhanced stabilities and their structures in the crystals could be determined by X‐ray diffraction analyses. The enantiomerically pure [7]triangulane dialdehyde showed remarkable diastereoselectivity in the condensation with the two enantiomers of trans‐1,2‐diaminocyclohexane: only one of the enantiomers gave a [2+2] macrocyclization product. Circular dichroism measurements combined with computational analysis show that the lowest energy electronic transition in these cyclic oligoimines is of n–π* type.
Assessing the Mechanical Properties of a Molecular Spring
Authors: Berni Emanuela , Kauffmann Brice , Bao Chunyan , Lefeuvre Julien , Bassani Dario M. , Huc Ivan 
Published: July 2007

AbstractWe report on the dramatic effect of increasing helix diameter on the hybridization of oligopyridine‐dicarboxamide strands into double helices. Upon replacing a single pyridine by a 1,8‐diazaanthracene unit within an oligomeric strand, a 4.7 Å enlargement of the helix diameter occurs parallel to the long anthracene axis. This structure change results in a spectacular stabilization of the double helical hybrids derived from these strands (factors of over 107). Detailed investigations of the hybridization process using X‐ray crystallography, NMR, fluorescence measurements and molecular mechanics calculations allowed us to assign the duplex stabilization to two enthalpic effects. First, the increase in diameter results in an augmented surface, involved in intermolecular π–π stacking. Second, the enlarged diameter leads to a lower tilt angle of the helical strand, with respect to the helix axis, which in turn results in smaller dihedral angles at the aryl–amide linkages and thus a considerably lowered enthalpic cost of the spring‐like extension of the strands during the hybridization process. These results provide novel insights into how subtle tuning of molecular components may result in considerable and rationalizable changes in double helical supramolecular architectures.
A randomized controlled trial to evaluate the effectiveness of a brief, behaviorally oriented intervention for cancer‐related fatigue
Authors: Armes Jo , Chalder Trudie , Addington‐Hall Julia , Richardson Alison , Hotopf Matthew 
Published: July 2007

AbstractBACKGROUND.It has been shown that nonpharmacologic interventions are effective management techniques for cancer‐related fatigue (CRF) in cancer survivors. However, few studies have investigated their effectiveness in patients who are receiving chemotherapy. In this study, the authors tested the effectiveness of a brief behaviorally oriented intervention in reducing CRF and improving physical function and associated distress in individuals who were receiving chemotherapy.METHODS.For this randomized controlled trial, 60 patients with cancer were recruited and received either usual care or the intervention. The intervention was delivered on an individual basis on 3 occasions over a period from 9 weeks to 12 weeks, and the objective of the intervention was to alter fatigue‐related thoughts and behavior. Primary outcomes were assessed as follows: CRF using the Visual Analogue Scale‐Global Fatigue; physical functioning using the European Organization for Research and Treatment of Cancer Quality‐of‐Life Core 30 Questionnaire, and CRF‐associated distress using the Fatigue Outcome Measure. Assessments were made on 4 occasions: at baseline (T0), at the end of chemotherapy (T1), 1 month after chemotherapy (T2), and 9 months after recruitment (T3). Normally distributed data were analyzed usingttests and random‐slope/random‐intercept mixed models.RESULTS.The intervention demonstrated a trend toward improved CRF, although this effect was reduced once confounders had been controlled statistically. There was a significant improvement in physical functioning (coefficient, 10.0; 95% confidence interval, 2.5–17.5;P= .009), and this effect remained once the confounding effects of mood disturbance and comorbid disorders were controlled statistically. No decrease in fatigue‐related distress was detected.CONCLUSIONS.The behaviorally oriented intervention brought about significant improvements in physical functioning, indicated a trend toward improved CRF, but detected no effect for fatigue‐related distress. Cancer 2007. © 2007 American Cancer Society.
Dkk3‐Cre BAC transgenic mouse line: a tool for highly efficient gene deletion in retinal progenitor cells
Authors: Sato Shigeru , Inoue Tatsuya , Terada Koji , Matsuo Isao , Aizawa Shinichi , Tano Yasuo , Fujikado Takashi , Furukawa Takahisa 
Published: July 2007

AbstractTo establish the genetic tools for conditional gene deletion in mouse retinal progenitors, we generated a Dkk3‐Cre transgenic mouse line using bacterial artificial chromosome (BAC) transgenesis. Cre recombination efficiency in vivo was assayed by crossing this transgenic line, termed BAC‐Dkk3‐Cre, with the CAG‐CAT‐Z reporter line. This BAC‐Dkk3‐Cre line showed Cre recombinase activity in most retinal progenitors. Cre activity was detectable from embryonic day 10.5 (E10.5) and generally restricted to the retina during embryogenesis. To verify that BAC‐Dkk3‐Cre mice successfully circumvented lethality, we generated Otx2flox/flox/BAC‐Dkk3‐Cre+ mice as Otx2 conditional knockout mice. The Otx2flox/flox/BAC‐Dkk3‐Cre+ mice were viable, and their retina showed loss of mature cell‐type markers of photoreceptor cells, bipolar cells, and horizontal cells, in contrast, amacrine‐like cells noticeably increased. Thus, the BAC‐Dkk3‐Cre transgenic mouse line provides a powerful tool for generating conditional knockout mouse lines for studying loss of gene functions in the developing retina. genesis 45:502–507, 2007. © 2007 Wiley‐Liss, Inc.
Insights into function and regulation of small heat shock protein 25 (HSPB1) in a mouse model with targeted gene disruption
Authors: Huang Lei , Min Jin‐Na , Masters Shane , Mivechi Nahid F. , Moskophidis Demetrius 
Published: July 2007

AbstractThe mammalian small heat shock protein (sHSPs) family is comprised of 10 members and includes HSPB1, which is proposed to play an essential role in cellular physiology, acting as a molecular chaperone to regulate diverse cellular processes. Whilst differential roles for sHSPs are suggested for specific tissues, the relative contribution of individual sHSP family members in cellular and organ physiology remains unclear. To address the function of HSPB1 in vivo and determine its tissue‐specific expression during development and in the adult, we generated knock‐in mice where the coding sequence ofhspb1is replaced by alacZreporter gene.Hspb1expression marks myogenic differentiation with specific expression first confined to developing cardiac muscles and the vascular system, and later in skeletal muscles with specific expression at advanced stages of myoblast differentiation. In the adult,hspb1expression was observed in other tissues, such as stratified squamous epithelium of skin, oronasal cavity, tongue, esophagus, and uterine cervix but its expression was most prominent in the musculature. Interestingly, in cardiac musclehsbp1expression was down‐regulated during the neonatal period and maintained to a relatively low steady‐level throughout adulthood. Despite this widespread expression,hspb1−/−mice were viable and fertile with no apparent morphological abnormalities in tissues under physiological conditions. However, at the cellular level and under stress conditions (heat challenge), HSPB1 act synergistically with the stress‐induced HSPA1 (HSP70) in thermotolerance development, protecting cells from apoptosis. Our data thus indicate a nonessential role for HSPB1 in embryonic development and for maintenance of tissues under physiological conditions, but also shows that it plays an important role by acting synergistically with other HSPs during stress conditions to exert cytoprotection and anti‐apoptotic effects. genesis 45:487–501, 2007. Published 2007 Wiley‐Liss, Inc.
The TGF‐β pseudoreceptor gene Bambi is dispensable for mouse embryonic development and postnatal survival
Authors: Chen Jianquan , Bush Jeffrey O. , Ovitt Catherine E. , Lan Yu , Jiang Rulang 
Published: July 2007

AbstractThe Bambi (Bmp and activin membrane‐bound inhibitor) gene encodes a transmembrane protein highly similar in amino acid sequence to transforming growth factor‐beta (TGF‐β receptors, however, the Bambi intracellular domain is short and lacks a serine/threonine‐kinase domain that is essential for transducing TGF β signaling. Previous biochemical assays showed that Bambi interacts directly with BMP receptors and antagonizes BMP signaling. Interestingly, the expression of Bambi largely overlaps, both temporally and spatially, with that of Bmp4 during early embryonic development in Xenopus, zebrafish, and mice, which led to the hypothesis that Bambi may function to regulate BMP signaling during embryogenesis. To directly analyze the roles of Bambi during embryonic development, we generated mice carrying a conditional allele of Bambi, Bambiflox, with loxP sequences flanking the first exon that encodes the N‐terminus and signal peptide region of the Bambi protein. Mice homozygous for this targeted conditional allele appear normal and fertile. We crossed the Bambiflox/+ mice to the EIIa‐Cre transgenic mice and generated mice carrying deletion of the first exon of the Bambi gene. Surprisingly, mice homozygous for the deleted allele were viable, fertile and did not exhibit any discernible developmental defect. Our data exclude an essential role for Bambi in mouse embryonic development and postnatal survival. genesis 45:482–486, 2007. © 2007 Wiley‐Liss, Inc.
Synthesis and Catalytic Applications of Chiral Hydridoiridium(III) Complexes with Diamine/Bis(monophosphane) and Diamine/Diphosphane Coordination
Authors: Dahlenburg Lutz , Menzel Ralf , Heinemann Frank W. 
Published: July 2007

AbstractThe P2/N2‐coordinated cis‐dihydridoiridium(III) chelate complexes (OC‐6‐13)‐[IrH2(H2N∩NH2)(PR3)2]BF4 [PR3 = PPh3, H2N∩NH2 = 1,2‐(H2N)2C6H4 (1a); (1R,2R)‐(H2N)2C6H10 {(R,R)‐dach} (1b); (R)‐2,2′‐diamino‐1,1′‐binaphthyl {(R)‐dabin} (1c); PR3 = PiPr3, H2N∩NH2 = (R,R)‐dach (2a), (R)‐dabin (2b); PR3 = PCy3, H2N∩NH2 = (R)‐dabin (3)] were obtained by treating the respective diamine ligands with labile precursors such as [IrH2(OCMe2)2(PPh3)2]BF4, [(η4‐1,5‐C8H12)Ir(PPh3)2]BF4, or [IrH5(PR3)2]/HBF4 (R = iPr, Cy). While oxidative addition of HCl to [(η4‐1,5‐C8H12)Ir{(S,S)‐bdpcp}]BF4 [(S,S)‐bdpcp = (1S,2S)‐(Ph2P)2C5H8] yields the usual mononuclear adduct [(η4‐1,5‐C8H12)Ir(H)(Cl){(S,S)‐bdpcp}]BF4 (4), similar treatment of [(η4‐1,5‐C8H12)Ir{(R)‐binap}]BF4 furnishes the triply chlorido‐bridged diiridium complex [{(R)‐binap}2Ir2H2(μ‐Cl)3]BF4 (5). Opening of the μ‐Cl bridges of 5 by N,N nucleophiles was used to synthesise the three diamine/(R)‐binap complexes [Ir(H)(Cl)(H2N∩NH2){(R)‐binap}]BF4 [H2N∩NH2 = (1R,2R)‐H2NCH(Ph)CH(Ph)NH2 {(R,R)‐dpen} (6a), (R,R)‐dach (6b), and H2NCMe2CMe2NH2 {tmen} (6c). Whereas the dihydrides [IrH2{(R,R)‐dach}(PR3)2]BF4 and [IrH2{(R)‐dabin}(PR3)2]BF4 (R = iPr, Ph) are only poor (pre)catalysts for the enantioselective hydrogenation of acetophenone, complexes 6a–c catalyze the formation of 1‐phenylethanol in good enantiomeric excess [eemax = 82–84 % (S)] in the presence of base. The crystal structures of 1a, 4, and 5 have been determined. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Unprecedented Rearrangement of Molybdenum(VI) Oxide to (μ2‐Oxido)bis[dioxidomolybdenum(VI)] Hexamolybdate
Authors: Pedrosa María R. , Aguado Rafael , Díez Virginia , Escribano Jaime , Sanz Roberto , Arnáiz Francisco J. 
Published: July 2007

AbstractMoO2(acac)2 reacts with water, amides, sulfoxides, and phosphane oxides in aqueous methanol, under mild conditions, to afford a new family of molybdates of composition [Mo2O5L6][Mo6O19] (L = H2O, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, dibutyl sulfoxide, tributylphosphane oxide, triphenylphosphane oxide, hexamethylphosphoramide). The crystal and molecular structures of the two first structurally characterized (μ2‐oxido)bis[dioxidomolybdenum(VI)] hexamolybdates were established by X‐ray diffraction analysis. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
A Convenient Route to 2‐Hydroxy‐ and 2,15‐Dihydroxyhexahelicene
Authors: Teplý Filip , Stará Irena G. , Starý Ivo , Kollárovič Adrian , Luštinec Daniel , Krausová Zuzana , Šaman David , Fiedler Pavel 
Published: July 2007

Abstract2‐Hydroxy‐ and 2,15‐dihydroxyhexahelicene were synthesised from simple benzene and naphthalene building blocks by intramolecular CoI‐ or Ni0‐catalysed [2+2+2] cycloisomerisation of CH3O‐substituted aromatic triynes. This approach avoids vexing photodehydrocyclisation of stilbene‐type precursors, providing thus a useful alternative to classical procedures. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Carbon Electrodes Modified by Nanoscopic Iron(III) Oxides to Assemble Chemical Sensors for the Hydrogen Peroxide Amperometric Detection
Authors: Hrbac Jan , Halouzka Vladimir , Zboril Radek , Papadopoulos Kyriakos , Triantis Theodor 
Published: July 2007

AbstractIn this study we show that nanoparticles of various ferric oxides (hematite, maghemite, amorphous Fe2O3, β‐Fe2O3 and ferrihydrite) incorporated into carbon paste exhibit electro‐catalytic properties towards hydrogen peroxide reduction. The modified paste electrode performances were evaluated and compared with those obtained with Prussian Blue‐modified carbon paste electrode, which represents an excellent chemical mediator towards the H2O2 redox reaction (as widely described in literature). The best catalytic activity was found for carbon paste modified by amorphous ferric oxide with 2–4 nm particle size, which was further tested for possible application as hydrogen peroxide sensor. At pH 7, the limit of detection was 2×10−5 M H2O2 (S/N=3), the calibration curves were linear upto 8.5 mM H2O2 (R2=0.998), the measurement reproducibility (RSD=97%, n=4), the interelectrode reproducibility (RSD=16%, nelectrodes=5) and <3 s response time.
Evaluation of enantioselective binding of basic drugs to plasma by ACE
Authors: Martínez‐Gómez María Amparo , Villanueva‐Camañas Rosa María , Sagrado Salvador , Medina‐Hernández Maria José 
Published: July 2007

AbstractThe present paper deals with the evaluation of the stereoselective binding of antihistamines (brompheniramine, chlorpheniramine, hydroxyzine, orphenadrine and phenindamine), phenothiazines (promethazine and trimeprazine) and a local anesthetic (bupivacaine) to human plasma proteins. Since all of them are drugs highly bound to proteins, a methodology to determine the bound fraction of each drug enantiomer was proposed. This methodology includes the incubation of samples containing plasma and racemic drug, ultrafiltration of the mixture and the chiral separation of enantiomers in the bound drug fraction using affinity EKC (AEKC)‐partial filling technique and HSA as chiral selector. The results shown in this paper represent the first evidence of the enantioselective binding of some antihistamines such as brompheniramine, hydroxyzine, orphenadrine and phenindamine and the phenothiazines, promethazine and trimeprazine, to human plasma proteins. The binding of phenindamine to plasma presented the highest enantioselectivity (ES) (ES = 2.5) followed by trimeprazine (ES = 1.5) and promethazine (ES = 1.4).
IFN‐γ‐induced IDO and WRS expression in microglia is differentially regulated by IL‐4
Authors: Yadav Manisha C. , Burudi E. M. E. , Alirezaei Mehrdad , Flynn Claudia C. , Watry Debbie D. , Lanigan Caroline M. , Fox Howard S. 
Published: July 2007

AbstractIndoleamine 2,3‐dioxygenase (IDO), a tryptophan catabolizing enzyme, has been implicated in the pathogenesis of various neurological disorders. IDO expression is induced by IFN‐γ and leads to neurotoxicity by generating quinolinic acid. Additionally, it inhibits the immune response through both tryptophan depletion and generating other tryptophan catabolites. IL‐4 and IL‐13 have been shown to control IDO expression by antagonizing the effects of IFN‐γ in different cell types. Here, we investigated the effects of these cytokines on IDO expression in microglia. Interestingly, we observed that both IL‐4 and IL‐13 greatly enhanced IFN‐γ‐induced IDO expression. However, tryptophanyl‐tRNA synthetase (WRS), which is coinduced with IDO by IFN‐γ, is downregulated by IL‐4 and IL‐13. The effect of IL‐4 and IL‐13 was independent of STAT‐6. Modulation of IDO but not WRS was eliminated by inhibition of protein phosphatase 2A (PP2A) activity. The phosphatidylinositol 3‐kinase (PI3K) pathway further differentiated the regulation of these two enzymes, as inhibiting the PI3K pathway eliminated IFN‐γ induction of IDO, whereas such inhibition greatly enhanced WRS expression. These findings show discordance between modulations of expression of two distinct enzymes utilizing tryptophan as a common substrate, and raise the possibility of their involvement in regulating immune responses in various neurological disorders. © 2007 Wiley‐Liss, Inc.
Innate immune response to double-stranded RNA in biliary epithelial cells is associated with the pathogenesis of biliary atresia
Authors: Harada Kenichi , Sato Yasunori , Itatsu Keita , Isse Kumiko , Ikeda Hiroko , Yasoshima Mitsue , Zen Yoh , Matsui Akira , Nakanuma Yasuni 
Published: July 2007

Abstract

Infections of Reoviridae consisting of a double-stranded RNA (dsRNA) genome are a possible cause of biliary atresia (BA). The aim of the present study is to clarify the pathophysiological function of dsRNA viruses in the pathogenesis of BA. The expression of dsRNA pattern-recognizing receptors, Toll-like receptor 3 (TLR3), retinoic acid inducible gene I (RIG-I), melanoma differentiation-associated gene-5 (MDA-5), and dsRNA-activated protein kinase R (PKR) was constitutively detected in cultured human biliary epithelial cells (BECs). Stimulation with polyinosinic-polycytidylic acid [poly(I:C), a synthetic analog of viral dsRNA] induced the activation of transcription factors [nuclear factor (NF)-κB and interferon regulatory factor 3 (IRF3)] and the production of interferon-β1 (IFN-β1) and MxA as potent antiviral responses. Moreover, poly(I:C) up-regulated the expression of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL), and both poly(I:C) and TRAIL reduced the viability of cultured human BECs by enhancing apoptosis. Experiments

in vivo

using tissue sections of extrahepatic bile ducts from patients with BA and controls (choledochal cysts and nonbiliary diseases) showed that the activation of NF-κB, interferon regulatory factor-3 (IRF-3), and PKR, and the enhancement of TRAIL and single-stranded DNA (ssDNA)–positive apoptosis were significant in BA, although extrahepatic bile ducts diffusely and constantly expressed TLR3 in all diseases.

Conclusion

: dsRNA viruses could directly induce the expression of TRAIL and apoptosis in human biliary epithelial cells as a result of the biliary innate immune response, supporting the notion that Reoviridae infections are directly associated with the pathogenesis of cholangiopathies in cases of BA. (HEPATOLOGY 2007.)
The effects of 48 weeks of rosiglitazone on hepatocyte mitochondria in human nonalcoholic steatohepatitis
Authors: Caldwell Stephen H. , Patrie James T. , Brunt Elizabeth M. , Redick Jan A. , Davis Christine A. , Park Sang H. , Neuschwander-Tetri Brent A. 
Published: July 2007

Abstract

Rosiglitazone, a thiazolidinedione peroxisome proliferator-activated receptor gamma ligand, reduces disease activity in nonalcoholic steatohepatitis (NASH), a disease associated with hepatocyte mitochondrial crystalline inclusions that are not seen in animal models of NASH. In human and animal studies of adipose tissue, thiazolidinediones may induce mitochondrial biogenesis and associated morphological changes. To determine if rosiglitazone alters the hepatocyte mitochondrial morphology in human NASH, we prospectively and systematically examined liver biopsies from human subjects with NASH before and after 48 weeks of rosiglitazone by transmission electron microscopy. Twenty patients (body mass index = 34 ± 7) were studied. Four coded sections from each of 20 pretherapy biopsies and each of 20 posttherapy biopsies were examined by transmission electron microscopy. The total hepatocyte mitochondria and crystal-containing mitochondria were counted, and semiquantitative scoring was performed for macrosteatosis, microsteatosis, dilated endoplasmic reticulum, apoptosis, Mallory bodies, and hepatocyte enlargement. The total mitochondria count was unchanged after therapy, but there was a significant increase in crystal-containing mitochondria from 4.0% (95% confidence interval = 1.8-8.8) to 7.2% (95% confidence interval = 3.9-12.6; odds ratio = 1.80;

P

= 0.04) after the treatment with rosiglitazone. Macrosteatosis (

P

< 0.001) and Mallory bodies (

P

= 0.05) significantly decreased, but no change was evident in microsteatosis, cellular enlargement, dilated endoplasmic reticulum, or apoptosis.

Conclusion:

Rosiglitazone therapy of NASH is associated with increased crystalline inclusions in hepatocyte mitochondria. Whether these are adaptive or pathological remains unknown, and further studies are warranted to assess hepatic mitochondrial function during thiazolidinedione therapy for NASH. (HEPATOLOGY 2007.)
Influence of the tyrosine kinase inhibitors STI571 (Glivec®), lavendustin A and genistein on human mast cell line (HMC‐1560) activation
Authors: Löber Kristin , Alfonso Amparo , Escribano Luis , Botana Luis M. 
Published: July 2007

AbstractThe human mast cell line (HMC‐1560) was used to study the effects of tyrosine kinase (TyrK) inhibition on histamine release in consequence of intracellular Ca2+ or pH changes. This is important since the TyrK inhibitor STI571 (Glivec®) inhibits proliferation and induces apoptosis in HMC‐1560. HMC‐1560 cells have a mutation in c‐kit, which leads to a permanent phosphorylation of the KIT protein and their ligand‐independent proliferation. The TyrK inhibitors STI571, lavendustin A and genistein decrease spontaneous histamine release in 24‐h pre‐incubated cells. Results are compared with those of the mast cell stabiliser cromoglycic acid, which also drops spontaneous histamine release. When exocytosis is stimulated by alkalinisation, STI571 pre‐incubated cells release more histamine than non‐pre‐incubated cells. Alkalinisation‐induced histamine release reaches still higher levels in STI571 cells with activated protein kinase C (PKC) by PMA. We do not observe modifications on histamine release in cells, treated with PKC inhibitors (rottlerin, Gf109203 or Gö6976). Lavendustin A‐ and genistein 24‐h incubated cells behave similar to STI571 cells, whereas cromoglycic acid does not show effects after stimulation with alkalinisation. Stimulation of exocytosis with the Ca2+ ionophore ionomycin does not modify histamine response in TyrK inhibited cells. Ca2+ and pH changes are observed after long‐time incubation with STI571. Results show that pH is still higher in STI571 pre‐incubated cells after alkalinisation with NH4Cl, whereas intracellular Ca2+ concentration remains stable. This work further strength the importance of pHi as a cell signal and suggest that STI571 has transduction pathways in common with other TyrKs. J. Cell. Biochem. 103: 1076–1088, 2008. © 2007 Wiley‐Liss, Inc.