bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metallkomplexe mit biologisch wichtigen Liganden. CLXV N,O‐Chelat‐Komplexe von α‐Aminosäureanionen mit cyclopalladiertem N,N‐Dimethylferrocencarbothiamid
Authors: Hauck Thomas , Sünkel Karlheinz , Beck Wolfgang 
Published: October 2006

AbstractMetal Complexes of Biologically Important Ligands. CLXV N,O‐Chelate Complexes of α‐Amino Acid Anions with Cyclopalladated N,N‐DimethylferrocenecarbothioamideA short literature review on the reactions of various chlorobridged complexes with α‐aminoacidates, α‐amino acid esters, peptide ester or derivatives of amino acids is given. The chloro bridged o‐palladated N,N‐dimethylferrocenecarbothioamide [(fct)Pd(μ‐Cl)2Pd(fct)] reacts with the anions of glycine, L‐alanine, L‐proline, L‐valine, L‐phenylalanine, L‐leucine, L‐isoleucine to give the N,O‐chelates [(fct)Pd(N,O‐α‐aminocarboxylate)] (1–7). Due to the planary chirality of the unsymmetrically disubstituted cyclopentadienyl iron moiety of fct the complexes 2–7 with optically active amino acidate ligands are formed as a mixture of two diastereoisomers, which can be detected by their NMR spectra.
Neue Polyiodide des Caesiums mit Doppel‐ und Tripeldecker‐Kationen, [Cs(Benzo‐18‐Krone‐6)2]Ix und [Cs2(Benzo‐18‐Krone‐6)3](Ix)2 (x = 3, 5)
Authors: Herzmann Nina , Pantenburg Ingo , Müller Ingrid , Tyrra Wieland , Meyer Gerd 
Published: October 2006

AbstractNew Polyiodides of Cesium containing Double and Triple Decker Cations, [Cs(benzo‐18‐crown‐6)2]Ix and [Cs2(benzo‐18‐crown‐6)3](Ix)2 (x = 3, 5)[Cs(b18c6)2]Ix (x = 3 (1), 5 (3)) and [Cs2(b18c6)3](Ix)2 (x = 3 (2), 5 (4)) (b18c6 = benzo‐18‐crown‐6) have been synthesized by the reaction of benzo‐18‐crown‐6 (C16H24O6), cesium iodide (CsI) and iodine (I2) in acetonitrile (1), ethanol/dichloromethane (2, 4) and 2‐methoxyethanol (3). Their crystal structures were determined on the basis of single crystal X‐ray data {(1): monoclinic, C2/c, Z = 4, a = 2048.8(5), b = 1329.5(5), c = 1588.7(5) pm, β = 110.23(1)°; (2): monoclinic, C2/c, Z = 4, a = 2296.0(1), b = 2092.7(1), c = 1373.6(1) pm, β = 100.21(1)°; (3): monoclinic, P21/n, Z = 4, a = 1586.3(1), b = 1745.5(1), c = 1608.6(1) pm, β = 92.37(1)°; (4): triclinic, ${\rm P}{\bar 1}$, Z = 2, a = 1241.7(1), b = 1539.8(2), c = 1938.4(2) pm, α = 91.15(1), β = 100.53(1), γ = 95.26(1)°}. As expected, double decker cations centered by Cs atoms, [Cs(b18c6)2]+, are found in the structures of (1) and (3). In contrast, the triple decker cation found in (2) and (4) is less common. The triiodide anions of (1) and (2) can be regarded as normal and the chain‐type pentaiodide anions of (3) and (4) fall into the known systematic sequence of these anions. The differences in the connectivity of the crystallographically independent I5− anions in (4) are surprising with respect to the fact that, so far, independent pentaiodide anions do not show variations in their scheme of connectivity within one crystal structure.
[(Ph3Sn)3VO4]·CH3CN und [(Ph3Sn)3VO4]·2 DMF, Triphenylzinnvanadate mit neuartigen Kettenstrukturen
Authors: Herntrich Tobias , Merzweiler Kurt 
Published: October 2006

Abstract[(Ph3Sn)3VO4]·CH3CN and [(Ph3Sn)3VO4]·2 DMF, Triphenyltin Vanadates with Novel Chain StructuresThe reaction of Na3VO4 with Ph3SnCl in a water/CH2Cl2 mixture leads to the formation of [(Ph3Sn)3VO4] (1). Recrystallization of 1 from toluene/CH3CN gives pale yellow crystals of [(Ph3Sn)3VO4]·CH3CN (2). 2 crystallizes as coordination polymer which consists of infinite chains composed of corner‐sharing VO4 tetrahedra and Ph3SnO2 trigonal bipyramides. Additionally the VO4 groups are connected to two terminal SnPh3‐Groups containing tin atoms in a tetrahedral environment. [(Ph3Sn)3VO4]·2 DMF (3) which is obtained from Na3VO4 and Ph3SnCl in a water/DMF mixture contains a polymeric chain structure similar to 2 and additionally one of the terminal SnPh3 groups is coordinated to a DMF solvent molecule.
CrB‐Type Phases in the Tb‐Zr‐Al‐Si System
Authors: Muts Nataliya , Gladyshevskii Roman 
Published: October 2006

AbstractThe crystal structures of Tb(Al0.15Si0.85), (Tb0.70Zr0.30)(Al0.17Si0.83) and Zr(Al0.22Si0.78) have been refined from single‐crystal X‐ray diffraction data. The three compounds crystallize with CrB‐type structures (Pearson symbol oS8, space group Cmcm): Tb(Al0.15Si0.85): a = 4.2715(5), b = 10.5595(15), c = 3.8393(5) Å; (Tb0.70Zr0.30)(Al0.17Si0.83): a = 4.163(2), b = 10.423(5), c = 3.8543(18) Å; Zr(Al0.22Si0.78): a = 3.7824(6), b = 10.0164(16), c = 3.7795(5) Å. The existence of a significant CrB‐type solid solution in the quaternary system Tb‐Zr‐Al‐Si, based on the ternary compound Tb(Al0.15Si0.85) and extending toward the solid solution based on the binary compound ZrSi in the Zr‐Al‐Si system, cannot be excluded.
Synthesis and Crystal Structure of [Methylzinc(2‐Pyridylethyl)(tert‐butyldimethylsilyl)amide] and its Hydrolysis Product
Authors: Westerhausen Matthias , Kneifel Alexander N. , Nöth Heinrich 
Published: October 2006

AbstractThe metallation of (2‐pyridylethyl)(tert‐butyldimethylsilyl)amine (1) with dimethylzinc yields quantitatively dimeric methylzinc (2‐pyridylethyl)(tert‐butyldimethylsilyl)amide (2). Hydrolysis reactions lead to the precipitation of trakis(methylzinc) bis{2‐pyridylethyl)(tert‐butyldimethylsilyl}amide](μ4‐oxide)] (3).
Roberts, Sackman, Ingoldsby et al
Authors: Palmer Peter 
Published: October 2006

JEREMY DALE ROBERTS: Winter Music; Croquis (selection); Oggetti – Omaggio à Morandi; Wieglied; Layers; Hamadryad. Hiroaki Takenouchi (pno), Dimitri Murrath (vla), Lontano c. Odaline de la Martinez. Lorelt LNT118.NICHOLAS SACKMAN: Scorpio; Time-piece (revised 2002); Cross hands; Koi; Sonata for trombone and piano (revised 1999), Sextet for wind. Fine Arts Brass Quintet and various artists. Metier MSV CD92049.TOM INGOLDSBY: Dialogues; Piano Sonata; Trio (Sonata for Violin, Viola and Piano); After the Eulogy. Adam Summerhayes (vln), Bridget Carey (vla), Alan Brown (pno), Catherine Summerhayes (pno), Clive Williamson (pno). Meridian CDE 84534.‘North Star’. Works by HUW WATKINS, DIANA BURRELL, JAMES MACMILLAN, RHIAN SAMUEL, RUTH BYRCHMORE, JOHN HAWKINS, ROBIN HOLLOWAY. Deborah Calland (tpt), William Whitehead (organ). Deux-Elles DXL 1097 (www.deuxelles.com).
Statins inhibit the dimerization of β-secretase via both isoprenoid- and cholesterol-mediated mechanisms
Authors: Parsons Richard B. , Price Gemma C. , Farrant Joanna K. , Subramaniam Daryl , Adeagbo-Sheikh Jubril , Austen Brian M. 
Published: October 2006

We have previously reported that protein lipidation in the form of palmitoylation and farnesylation is critical for the production of Aβ (amyloid β-peptide), the dimerization of β-secretase and its trafficking into cholesterol-rich microdomains. As statins influence these lipid modifications in addition to their effects on cholesterol biosynthesis, we have investigated the effects of lovastatin and SIMVA (simvastatin) at a range of concentrations chosen to distinguish different cellular effects on Aβ production and β-secretase structure and its localization in bHEK cells [HEK-293 cells (human embryonic kidney cells) transfected with the Asp-2 gene plus a polyhistidine coding tag] cells. We have compared the changes brought about by statins with those brought about by the palmitoylation inhibitor cerulenin and the farnesyltransferase inhibitor CVFM (Cys-Val-Phe-Met). The statin-mediated reduction in Aβ production correlated with an inhibition of β-secretase dimerization into its more active form at all concentrations of statin investigated. These effects were reversed by the administration of mevalonate, showing that these effects were mediated via 3-hydroxy-3-methylglutaryl-CoA-dependent pathways. At low (1 μM) statin concentrations, reduction in Aβ production and inhibition of β-secretase dimerization were mediated by inhibition of isoprenoid synthesis. At high (&gt;10 μM) concentrations of statins, inhibition of β-secretase palmitoylation occurred, which we demonstrated to be regulated by intracellular cholesterol levels. There was also a concomitant concentration-dependent change in β-secretase subcellular trafficking. Significantly, Aβ release from cells was markedly higher at 50 μM SIMVA than at 1 μM, whereas these concentrations resulted in similar reductions in total Aβ production, suggesting that low-dose statins may be more beneficial than high doses for the therapeutic treatment of Alzheimer's disease.
Different radiological findings with the same pathologic diagnosis due to different age in primary osteosarcoma
Authors: Lee S. Y. , Cho W. H. , Song W. S. , Park J. H. 
Published: October 2006

Purpose: To examine the imaging characteristics of osteoblastic osteosarcoma in older patients, we compared them with those in adolescents because the radiological features of osteosarcoma can be atypical in elderly patients. Material and Methods: 29 cases of adolescent patients and 12 patients older than 40 years of age were reviewed. All cases were pathologically confirmed as osteoblastic osteosarcoma. The comparative factors were tumor location, plain radiological features including periosteal reactions, and the degree of soft tissue mass with magnetic resonance (MR) imaging. Results: Older patients demonstrated frequent osteolytic findings (83.3%), and none of the tumors showed a significant major periosteal reaction. Seven (58.3%) of the 12 tumors had no soft tissue mass (grade 0) and only three (25%) had a grade 3 mass. The differences in the two groups were statistically significant ( P<0.001). Conclusion: In older patients, primary osteosarcoma should be considered when making a differential diagnosis of osteolytic lesions without any periosteal reactions and small soft-tissue extensions. In cases with atypical findings, the morphology of specimens (aspiration, core, or surgical biopsy) from the lesion should be recommended when making an accurate diagnosis.
HIV-related bronchopulmonary infection by pseudomonas aeruginosa in the HAART era: radiological findings
Authors: Busi Rizzi E. , Schininà V. , Bordi E. , Buontempo G. , Narciso P. , Bibbolino C. 
Published: October 2006

Purpose: To evaluate whether highly active antiretroviral therapy (HAART) modifies radiographic appearances of Pseudomonas aeruginosa bronchopulmonary infection in HIV-infected patients. P. aeruginosa is increasingly reported as a respiratory pathogen in HIV+ patients with very low levels of CD4 lymphocytes. Few studies have analyzed the radiological presentation of bronchopulmonary disease that occurs in HAART-treated patients. Material and Methods: We retrospectively reviewed the chest radiographs of 46 HIV-infected patients with bronchopulmonary diseases in which P. aeruginosa was the sole respiratory pathogen that was isolated. All cases were community-acquired infection. Twenty-four of the patients were on HAART treatment, and 22 were not. Chest radiographs were assessed for the presence and distribution of parenchymal consolidation, reticular or reticulonodular infiltrates, bronchial wall thickening, ground-glass opacities, cavitation, pleural effusion, and adenopathies. Statistical analysis was done using Epi-Info version 6 (CDC, Atlanta, GA, USA). Results: Normal chest radiographs were observed in 11 patients. Eight of these 11 (73%) were receiving HAART, and 3/11 (27%) were not. The most common radiographic abnormality was bronchopneumonia, present in 24 of 46 patients (52%): in 10 of 24 (42%) patients with HAART and 14 of 22 (64%) without. Cavitation was seen in 1 of 24 (4%) patients with HAART and in 5 of 22 (23%) without HAART. Conclusion: Cavitation was more frequent in patients that were not receiving HAART, and normal chest radiographs were more frequently seen in patients on HAART.
A second radioisotope injection enhances intraoperative sentinel node identification in breast cancer patients without visualized nodes on preoperative lymphoscintigraphy
Authors: Leikola J. P. , Leppänen E. A. , von Smitten K. A. J. , Leidenius M. H. K. 
Published: October 2006

Purpose: To evaluate the influence of a second radioisotope injection on the intraoperative success rate in patients with non-visualized axillary sentinel nodes (SN). Material and Methods: Altogether, 534 consecutive breast cancer patients with lymphoscintigraphy (LS) and SN biopsy and were included. An intratumoral injection of 99mTc-labeled human albumin colloid with a median dose of 93 MBq was applied. Forty-two of the 80 patients without axillary hot spots on LS received a second tracer injection with a median dose of 70 MBq. Results: The visualization rate of axillary SN was 454/534 (85%). The intraoperative SN identification rate was 97% in patients with and 69% in patients without visualized SN in the axilla ( P<0.00005), but the success rate was higher (88%) with a second radioisotope injection than without it (47%; P<0.0002). Conclusion: The failure rate in intraoperative SN identification was minimized using a second radioisotope injection in patients without axillary SN on LS.
A simplified method for continuous hepatic arterial port-catheter system placement not requiring catheter end-hole occlusion
Authors: Yamagami T. , Kato T. , Hirota T. , Yoshimatsu R. , Matsumoto T. , Nishimura T. 
Published: October 2006

Purpose: To evaluate the efficacy of hepatic port-catheter system placement without distal catheter tip occlusion or gastroduodenal artery embolization distal to the catheter tip. Material and Methods: A port-catheter system was percutaneously implanted in 29 patients (16 men, 13 women; mean age 65.6 years) with unresectable liver cancer. Persistent blood flow through the end hole of the catheter was verified immediately and 1–10 days after catheter placement. Results: In all cases, percutaneous port-catheter placement was successfully performed. In seven of 29 (24.1%) patients, flow through the end hole of the catheter was verified immediately after the procedure. However, no flow was seen 1–10 days after the procedure. Conclusion: It is not necessary to occlude the end hole of the catheter tip and embolize the gastroduodenal artery beyond the catheter tip when placing a port-catheter system for repeated hepatic arterial infusion chemotherapy.
Packing Capillary Columns for RP-HPLC
Authors: Moritz Robert 
Published: October 2006

INTRODUCTIONCapillary or microcolumn (<0.5-mm internal diameter [I.D.]) high-performance liquid chromatography (HPLC) is an extremely powerful technique for separating small quantities of proteins and peptides. This protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 μl/min) and gradients required to operate slurry-packed capillary columns. A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to a number of commercial UV detectors. Procedures are described for the fabrication of 0.32-mm I.D. polyimide-coated fused-silica columns, slurry-packed with reversed-phase chromatographic supports.
Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins: Figure 1.
Authors: Verhagen Anne 
Published: October 2006

INTRODUCTIONThis protocol describes the use of FLAG-epitope-tagged proteins for both small-scale analysis and large-scale coimmunoprecipitation of interacting proteins. When examining protein interactions, it is sometimes possible to immunoprecipitate an endogenous protein X directly, without using an epitope tag, if antibodies are available. The advantage of examining interactions of endogenously expressed proteins is that these are more likely to be physiological and less likely to be an artifact of overexpression. However, when scaling up the procedure for protein isolation and identification, epitope-tagged overexpressed protein X (e.g., FLAG-protein X) may be necessary for sufficient coimmunoprecipitating protein to be isolated.
Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis
Authors: Ji Hong 
Published: October 2006

INTRODUCTIONCultured mammalian cell lines are widely used as study models in biomedical research. The protocol described here has been used successfully for many human colon carcinoma cell lines and fibroblast cell lines. Although most cellular proteins can be extracted by this protocol, some DNA-binding proteins may not be recovered. If DNA-binding proteins are to be studied, a nuclease (e.g., Benzonase) should be included. Expression proteomic studies frequently require the detection of low-abundance proteins.
Radioisotopic Labeling of Eukaryotic Cell Proteins Using 35S
Authors: Ji Hong 
Published: October 2006

INTRODUCTIONRadioisotope labeling of proteins is an important technique in expression proteomics studies for detecting low-abundance proteins. The procedure given below describes 35S labeling. After the labeling procedure described here, cell lysates can be prepared for analysis by 2D gel electrophoresis.
Radioisotopic Labeling of Eukaryotic Cell Proteins Using 32P
Authors: Ji Hong 
Published: October 2006

INTRODUCTIONRadioisotope labeling of proteins is an important technique in expression proteomics studies for detecting low-abundance proteins. The procedure given below describes a labeling technique using 32P labeling. After the labeling procedure described here, cell lysates can be prepared for analysis by 2D gel electrophoresis.
mlo‐based powdery mildew immunity: silver bullet or simply non‐host resistance?
Authors: HUMPHRY MATT , CONSONNI CHIARA , PANSTRUGA RALPH 
Published: October 2006

SUMMARYDurability and effectiveness against all genetic variants of a microbial species are hallmarks of so‐called plant ‘non‐host’ resistance. Highly effective immunity of monocotyledonous barley against the fungal powdery mildew pathogen, which is conferred by loss‐of‐function mutant alleles of the barley Mlo locus, likewise is a durable and broad‐spectrum type of resistance. Although this was long considered as being a barley‐specific phenomenon, recent findings indicate that mlo resistance can also occur in the distantly related dicotyledonous species Arabidopsis thaliana. Shared histological and phytopathological characteristics plus a conserved requirement for a set of genes in Arabidopsis mlo and non‐host powdery mildew resistance indicate a potential common mechanism for these two seemingly distinct types of immunity.
Early exposure to dichlorodiphenyldichloroethylene, breastfeeding and asthma at age six
Authors: Sunyer J. , Torrent M. , Garcia‐Esteban R. , Ribas‐Fitó N. , Carrizo D. , Romieu I. , Antó J. M. , Grimalt J. O. 
Published: October 2006

SummaryOur aims were to assess association of dichlorodiphenyldichloroethylene (DDE) with childhood asthma measured up to age 6 and the effect of DDE on the protective effect of breastfeeding on asthma. In addition, we attempted to assess the relevant time‐window of DDE exposure (i.e. at birth or at 4 years).All women presenting for antenatal care in Menorca, Spain over a 12‐month period beginning in mid‐1997 were invited to take part in a longitudinal study that included a yearly visit. Four hundred eighty‐two children were enrolled and 462 provided complete outcome data after 6.5 years of follow‐up. Organochlorine compounds were measured in cord serum of 402 (83%) infants and in blood samples of 285 children aged 4. We defined asthma as the presence of wheezing at age 6 and during any preceding year or doctor‐diagnosed asthma, and used skin prick test at age 6 to determine atopic status. Results At birth and 4 years of age, all children had detectable levels of DDE (median 1 ng/mL and 0.8 ng/mL, respectively). From birth to age 4, the mean DDE level among children with artificial feeding decreased by 72%, while among breastfed children it increased by 53%. Diagnosed asthma and persistent wheezing were associated with DDE at birth [odds ratio (OR) for an increase in 1 ng/mL, OR=1.18, 95% confidence interval (95% CI)=1.01–1.39 and OR=1.13, 95% CI=0.98–1.30, respectively], but not with DDE at 4 years. Neither breastfeeding nor atopy modified these associations (P>0.3). Breastfeeding protected against diagnosed asthma (OR=0.33, 95% CI=0.08–0.87) and wheezing (OR=0.53, 95% CI=0.34–0.82) in children with low and high DDE levels at birth. Conclusion In a community without known dichlorodiphenyltrichloroethane environmental releases, this study strengthens the evidence for an effect of DDE on asthma by measuring the disease at age 6 and does not support the hypothesis that DDE modifies the protective effect of breastfeeding on asthma.
Synergistic effects of fluticasone propionate and salmeterol on inhibiting rhinovirus‐induced epithelial production of remodelling‐associated growth factors
Authors: Volonaki E. , Psarras S. , Xepapadaki P. , Psomali D. , Gourgiotis D. , Papadopoulos N. G. 
Published: October 2006

SummaryBackground
Rhinoviruses (RV), the major trigger of acute asthma exacerbations, are able to infect bronchial epithelium and induce production of pro‐inflammatory, but also angiogenic and pro‐fibrotic mediators. Fluticasone propionate (FP) and salmeterol (S) are clinically effective and act synergistically in controlling persistent asthma; however, their effect on virus‐associated asthma is less clear.Aim
The aim of this study was to assess the individual and combined effects of FP and S on RV‐induced epithelial production of vascular endothelial growth factor (VEGF) and fibroblast growth factor‐2 (FGF‐2).Methods
Bronchial epithelial cells (BEAS‐2B) were exposed in vitro to RV and were subsequently treated with FP and S, at physiologically relevant concentrations, alone or in combination. VEGF and FGF‐2 were measured in the supernatants of these cultures using ELISA.Results
FP was able to reduce RV‐induced VEGF production in a dose‐dependent manner. S also induced a smaller reduction; addition of both factors inhibited VEGF synergistically. FGF‐2 production was not inhibited by either FP or S alone, but was significantly reduced when both substances were present in the culture.Conclusion
This study demonstrates that FP and S may synergistically inhibit the production of angiogenic and/or pro‐fibrotic factors that are induced after RV infection of BEAS‐2B and are implicated in airway remodelling, suggesting that this combination may represent an important therapeutic option on virus‐induced asthma.
Defining the T cell antigen proteome of wasp venom
Authors: Aslam A. , Kessler B. , Batycka M. , O'Callaghan C. A. , Misbah S. A. , Warrell D. A. , Ogg G. 
Published: October 2006

SummaryBackground
While modulation of T cell function is believed to be important in the successful acquisition of clinical tolerance during venom immunotherapy, little is known of the role of wasp venom specific T cell antigens.Objective
We sought comprehensively to characterize the T cell proteome for wasp venom to facilitate the future development of T cell‐based immunotherapeutic approaches.Methods
Using peripheral blood mononuclear cells from wasp venom‐allergic individuals and IL‐4 ELISPOT analysis, we characterized T cell responses to whole venom and gel filtration/ion exchange‐fractionated venom. Reactive fractions were purified and identified using highly sensitive electrospray ion‐trap mass spectrometry.Results
Wasp venom‐allergic individuals have detectable whole wasp venom‐specific T cells directly ex vivo, which show rapid IL‐4 effector function. T cell responses to gel filtration/ion exchange fractionated venom were dominated by responses to phospholipase A1, hyaluronidase and antigen 5.Conclusion
Although it is likely that there are many T cell antigens within wasp venom, the main responses are to proteins coincident with the known IgE‐binding proteins.