bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soluble immune complex triggering of a respiratory burst in macrophages: the role of complex aggregation at the phagocyte surface
Authors: Vieweg Reinout , Leslie Robert G. Q. 
Published: February 2007

AbstractThe capacity of small, soluble immune complexes (SIC) to rearrange into larger aggregates, through additional antibody‐antigen bridge formation, on a receptor‐bearing cell surface has previously been shown to play an important role in enhancing the uptake and ingestion of SIC by macrophages and has been implicated in the efficient clearance of SIC in vivo. In this report, we extend the study to investigate the role of SIC aggregation in triggering a respiratory burst in macrophages. SIC were found to be more efficient trigger signals than nonaggregating, antigen‐free IgG oligomers of similar average size in that they induced a more rapid respiratory burst response that was between 2‐ and 6‐fold greater in magnitude. The implications of these findings are discussed.
Small Group IIa–VIa Clusters and Related Systems: A Theoretical Study of Physical Properties, Reactivity, and Electronic Spectra
Authors: Srnec Martin , Zahradník Rudolf 
Published: February 2007

AbstractIn the monomeric form, the title systems assume unexpected optical properties, and as oligomers they can serve as candidates for molecular devices. In bulk they are attractive in the area of material science. A broad set of quantum chemical methods ranging from density functional theory by the Hartree–Fock method and the Møller–Plesset perturbation theory to the coupled‐cluster method, in connection with nonrelativistic Dunning's basis sets as well as with relativistic SDD basis sets were used. Electronic spectra were analyzed by means of time‐dependent DFT, with the symmetry‐adapted cluster configuration interaction and with the internally contracted multireference configuration interaction. The Douglas–Kroll–Hess quasirelativistic Hamiltonian served as a basis for the estimation of the role of relativistic effects. All the diatomics representing 25 elements of a matrix formed by the combination of group IIa (Be, ..., Ba) with group VIa (O, ..., Po) atoms were characterized by calculated bond length, valence vibration, dipole moment, and the Hartree–Fock frontier orbital energies. Calculated characteristics were confronted with experimental data. The geometry of the oligomers (from dimers through hexamers) was investigated systematically, and the nature of the located stationary points on the respective potential energy surfaces was established. Special attention was paid to the four lightest elements of the group IIa–VIa matrix, i.e. BeO, BeS, MgO, and MgS. As for electronic spectra, the systems of the first column (BeO, ..., BaO), the first row (BeO, ..., BePo), and the systems of the main diagonal (BeO, ..., BaPo) were calculated in the form of monomers and dimers. Analogous calculations were performed for a few group Ia–VIIa, IIIa–Va, IVa–IVa, and IIb–VIa systems. The group IIa–VIa and IIb–VIa diatomics exhibit electronic transitions in the visible region of the spectrum, and the longest wavelength bands are located in the near‐infrared region. MgO represents an extreme with the first band appearing at about 3500 cm–1, which strictly speaking makes the use of the Born–Oppenheimer approximation questionable. Whenever experimental transitions are available, the agreement between the calculated and observed band positions is good. Passing from monomers to oligomers is always associated with a significant hypsochromic shift in the first transition.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Solvothermal Synthesis, Crystal Structure, and Thermal Stability of Three‐Layered Thioantimonate(III) Complexes: [Ni(C3H10N2)3]Sb4S7, [C4H14N2]Sb8S13·H2O, and [C6H18N2]Sb10S16·H2O
Authors: Zhang Meng , Sheng Tian Lu , Huang Xi He , Fu Rui Biao , Wang Xin , Hu Sheng Min , Xiang Sheng Chang , Wu Xin Tao 
Published: February 2007

AbstractThree new thioantimonate(III) complexes [Ni(1,2‐PDA)3]Sb4S7 (1) (1,2‐PDA = 1,2‐propanediamine), [dmenH22+]Sb8S13·H2O (2) (dmen = N,N‐dimethylethylenediamine), and [deenH22+]Sb10S16·H2O (3) (deen = N,N‐diethylethylenediamine), prepared under solvothermal conditions, have been characterized by single‐crystal X‐ray diffraction, elemental analysis, and DTA‐TG measurements. In compound 1, a rectangle‐like Sb16S16 heteroring whose dimensions are about 8.1 × 14.7 Å is observed, this is the largest reported pore in layered thioantimonates to date. Further condensation of the Sb16S16 heterorings resulted in an unprecedented framework of the five‐atom thick SbxSyn– layer. In 2, a new member of SbnSn (n = 2, 3, 4, 8, 16, 30, 31, 32) heterocycles Sb15S15 heteroring has been obtained as the first example. In compound 3, the most particular feature is that when the secondary Sb–S bonds are considered the Sb2 atom becomes sevenfold coordinated.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
A 3‐D dielectrophoretic filter chip
Authors: Iliescu Ciprian , Xu Guolin , Loe Felicia Celeste , Ong Poh Lam , Tay Francis E. H. 
Published: February 2007

AbstractThe paper presents a 3‐D filter chip employing both mechanical and dielectrophoretic (DEP) filtration, and its corresponding microfabrication techniques. The device structure is similar to a classical capacitor: two planar electrodes, made from a stainless steel mesh, and bonded on both sides of a glass frame filled with round silica beads. The solution with the suspension of particles flows through both the mesh‐electrodes and silica beads filter. The top stainless steel mesh (with openings of 60 μm and wires of 30 μm‐thickness) provides the first stage of filtration based on mechanical trapping. A second level of filtration is based on DEP by using the nonuniformities of the electric field generated in the capacitor due to the nonuniformities of the dielectric medium. The filter can work also with DC and AC electric fields. The device was tested with yeast cells (Saccharomyces cerevisae) and achieved a maximal trapping efficiency of 75% at an applied AC voltage of 200 V and a flow rate of 0.1 mL/min, from an initial concentration of cells of 5×105 cells/mL. When the applied frequency was varieted in the range between 20 and 200 kHz, a minimal value of capture efficiency (3%) was notticed at 50 kHz, when yeast cells exhibit negative DEP and the cells are repelled in the space between the beads.
Lamination‐based rapid prototyping of microfluidic devices using flexible thermoplastic substrates
Authors: Paul Debjani , Pallandre Antoine , Miserere Sandrine , Weber Jérémie , Viovy Jean‐Louis 
Published: February 2007

AbstractTransposing highly sensitive DNA separation methods (such as DNA sequencing with high read length or the detection of point mutations) to microchip format without loss of resolution requires fabrication of relatively long (approx. 10 cm) microchannels along with sharp injection bands. Conventional soft lithography methods, such as mold casting or hot‐embossing in a press, are not convenient for fabricating long channels. We have developed a lamination‐based replication technique for rapid fabrication of sealed microfluidic devices with a 10 cm long, linear separation channel. These devices are fabricated in thin cyclo‐olefin copolymer (COC) plastic substrates, thus making the device flexible and capable of assuming a range of 3‐D configurations. Due to the good optical properties of COC, this new family of devices combines multiple advantages of planar microfluidics and fused‐silica capillaries.
Isotachophoresis of proteins in a networked microfluidic chip: Experiment and 2‐D simulation
Authors: Cui Huanchun , Dutta Prashanta , Ivory Cornelius F. 
Published: February 2007

AbstractThis paper reports both the experimental application and 2‐D simulation of ITP of proteins in a networked microfluidic chip. Experiments demonstrate that a mixture of three fluorescent proteins can be concentrated and stacked into adjacent zones of pure protein under a constant voltage of 100 V over a 2 cm long microchannel. Measurements of the isotachophoretic velocity of the moving zones demonstrates that, during ITP under a constant voltage, the zone velocity decreases as more of the channel is occupied by the terminating electrolyte. A 2‐D ITP model based on the Nernst–Planck equations illustrates the stacking and separation features of ITP using simulations of three virtual proteins. The self‐sharpening behavior of ITP zones dispersed by a T‐junction is clearly demonstrated both by experiment and by simulation. Comparison of 2‐D simulations of ITP and zone electrophoresis (ZE) confirms that ZE lacks the ability to resharpen protein zones after they pass through a T‐junction.
Three‐dimensional immersed boundary conditions for moving solids in the lattice‐Boltzmann method
Authors: Strack O. Erik , Cook Benjamin K. 
Published: February 2007

AbstractThis paper establishes the range of validity for a previously published three‐dimensional moving solid boundary condition for the lattice‐Boltzmann method. This method was reasonably formulated from a mass and momentum balance perspective, but was only verified for a small range of (primarily two‐dimensional) problems. One of the advantages of this boundary condition is that it offers resolution at the sub‐grid scale, allowing for accurate and stable calculation of the force and torque for solids which are moving through a lattice, even for small solid sizes relative to the computational grid size. We verify the boundary condition for creeping flows by comparison to analytical solutions that include both the force and the torque on fixed and moving spheres, and then follow this with comparisons to experimental and empirical results for both fixed as well moving spheres in inertial flows. Finally, we compare simulation results to numerical results of other investigators for the settling of an offset sphere and the drafting–kissing–tumbling of two sedimenting spheres. We found that an accurate calculation of the collision‐operator weighting used to obtain sub‐grid‐scale resolution was necessary in order to prevent spikes in the velocities, forces, and moments when solid objects cross‐computational cells. The wide range of comparisons collected and presented in this paper can be used to establish the validity of other numerical models, in addition to the one examined here. Published in 2007 by John Wiley & Sons, Ltd.
Computation of inviscid compressible flows with the V‐SGS stabilization and YZβ shock‐capturing
Authors: Rispoli Franco , Saavedra Rafael , Corsini Alessandro , Tezduyar Tayfun E. 
Published: February 2007

AbstractThe YZβ shock‐capturing technique was introduced recently for use in combination with the streamline‐upwind/Petrov–Galerkin formulation of compressible flows in conservation variables. The YZβ shock‐capturing parameter is much simpler than an earlier parameter derived from the entropy variables for use in conservation variables. In this paper, we propose to use the YZβ shock‐capturing in combination with the variable subgrid scale (V‐SGS) formulation of compressible flows in conservation variables. The V‐SGS method is based on an approximation of the class of SGS models derived from the Hughes variational multiscale method. We evaluate the performance of the V‐SGS and YZβ combination in a number of standard, 2D test problems. Compared to the earlier shock‐capturing parameter derived from the entropy variables, in addition to being much simpler, the YZβ shock‐capturing parameter yields better shock quality in these test problems. Copyright © 2007 John Wiley & Sons, Ltd.
Mutations of the PTPN11 gene in therapy‐related MDS and AML with rare balanced chromosome translocations
Authors: Christiansen Debes H. , Desta Frehiwet , Andersen Mette K. , Pedersen‐Bjergaard Jens 
Published: February 2007

AbstractActivating mutations of the PTPN11 gene encoding the SHP2 tyrosine phosphatase is the most common genetic abnormality in juvenile myelomonocytic leukemia and is sporadically observed in myelodysplasia (MDS) and acute myeloid leukemia (AML). An unselected series of 140 patients with therapy‐related MDS or AML were investigated for mutations of PTPN11 in Exons 3, 4, 8, and 13. Four cases had mutations of the gene; three of these had deletions or loss of chromosome arm 7q. Two cases had rare balanced translocations to chromosome band 21q22 with rearrangement of the RUNX1 gene and the other two patients had rare balanced translocations to chromosome band 3q26 with rearrangement of the EVI1 gene. The findings support cooperation between so called Class I and Class II mutations in leukemogenesis. © 2007 Wiley‐Liss, Inc.
Increasing genomic instability during premalignant neoplastic progression revealed through high resolution array‐CGH
Authors: Lai Lisa A. , Paulson Thomas G. , Li Xiaohong , Sanchez Carissa A. , Maley Carlo , Odze Robert D. , Reid Brian J. , Rabinovitch Peter S. 
Published: February 2007

AbstractChromosomal instability is regarded as an underlying mechanism of neoplastic progression, integral to the clonal selection and evolution that leads to cancer. We evaluated chromosomal instability in premalignant Barrett's esophagus tissue using high resolution Affymetrix mapping 100K SNP arrays as patients progressed through three molecular stages of disease—CDKN2ALOH only, CDKN2ALOH/TP53LOH, and CDKN2ALOH/TP53LOH with aneuploidy. Within individuals over time, we observed increases in both numbers and sizes of regions of LOH or copy number change. In the earliest CDKN2ALOH only samples, we detected few regions with both copy change and LOH, whereas copy loss and LOH were highly correlated in more advanced samples. These data indicate that genomic instability increases in severity and changes character during neoplastic progression. In addition, distinct patterns of clonal evolution could be discerned within a segment of Barrett's esophagus. Overall, this study illustrates that pre‐malignant disease can be associated with extensive instability and clonal dynamics that evolve from an initial stage characterized by small recombination‐based alterations to one with larger copy change events likely associated with mitotic instability. © 2007 Wiley‐Liss, Inc.
The impact of personality on training‐related aspects of motivation: Test of a longitudinal model
Authors: Rowold Jens 
Published: February 2007

AbstractA model that proposed dispositional influences on training‐related aspects of motivation was developed. More specifically, the model predicted influences of the Big Five personality variables on motivation to learn and transfer motivation, while controlling for general attitudes toward training. The model was tested empirically, drawing on a sample of ninety‐four employees from call centers who participated in a training program. Results indicated that motivation to learn was predicted by extraversion and agreeableness, and transfer motivation was predicted by motivation to learn, extraversion, and emotional stability. The impact of extraversion on transfer motivation was partially mediated by motivation to learn. In sum, the importance of dispositional factors in training research was explored and clarified. Implications for theory and practice are discussed.
Strategies for publishing in scholarly HRD journals
Authors: Chisholm Kelley 
Published: February 2007

AbstractSubmitting a manuscript to a journal and having that manuscript published is a crucial step in sustaining or furthering an academic career. In addition, scholar‐practitioners and others interested in furthering their discipline's knowledge base find publishing in scholarly journals a critical activity. Success in publishing research in peer‐reviewed journals can determine whether professionals obtain or retain tenure‐track positions, as well as add to the base of knowledge that may be disseminated in the literature (Thompson, 1995). This Forum offers advice on preparing manuscripts for publication, describes the peer review process, and explains the most common reasons that manuscripts are not accepted.
Geochemistry of meteoric diagenesis in carbonate islands of the northern Bahamas: 1. Evidence from field studies
Authors: Whitaker Fiona F. , Smart Peter L. 
Published: February 2007

AbstractProcesses driving carbonate diagenesis in islands of the northern Bahamas are investigated using major ion, dissolved oxygen and dissolved organic carbon analyses of water samples from surface and ground waters, and measurements of soil gas P. Meteoric waters equilibrate with aragonite, but reactions are water controlled rather than mineral‐controlled and drive dissolution rather than concurrent precipitation of calcite. Surface runoff waters equilibrate with atmospheric P and rapidly recharge the vadose zone, limiting subaerial bedrock dissolution to only 6·6–15 mg l−1 Ca. P of soil gas measured in the summer wet season ((7·4 ± 3·7) × 10−3 atm) is elevated compared with that of the atmosphere, despite the thin skeletal organic nature of the soil and the discontinuous soil cover. Soil waters retained in surface pockets are equilibrated with respect to aragonite and have dissolved 51 ± 19 mg l−1 Ca. This is substantially less than the 93 ± 18 mg l−1 Ca in samples from pumping boreholes that sample meteoric waters from the freshwater lens. The high P of the freshwater lens ((16 ± 8·3) × 10−3 atm for pumping boreholes) suggests that significant additional CO2 may be derived by oxidation of soil‐ and surface‐derived organic carbon within the lens. The suboxic nature of the majority of the freshwater lens and the observed depletion in sulphate support this suggestion, and indicate that both aerobic and anaerobic oxidation may take place. Shallow lens samples from observation boreholes are calcite supersaturated and have a lower P than deeper lens waters, indicating that CO2 degasses from the water table, driving precipitation of calcite cements. We suggest that the geochemical evolution of waters in the vadose zone and upper part of the freshwater lens may be determined by the presence of a body of ground air with P controlled by production in the freshwater lens and soil and by degassing to the atmosphere. Copyright © 2007 John Wiley & Sons, Ltd.
Direct evidence for rapid and selective induction of tumor neovascular permeability by tumor necrosis factor and a novel derivative, colloidal gold bound tumor necrosis factor
Authors: Farma Jeffrey M. , Puhlmann Markus , Soriano Perry A. , Cox Derrick , Paciotti Giulio F. , Tamarkin Lawrence , Alexander H. Richard 
Published: February 2007

AbstractTumor necrosis factor (TNF) causes regression of advanced cancers when used in isolation perfusion with melphalan; evidence suggests these effects are mediated via selective yet uncharacterized actions on tumor neovasculature. A novel derivative, colloidal gold bound TNF (cAu‐TNF) has been shown to have similar antitumor effects as native TNF with less systemic toxicity in mice. These studies were done to determine their effects on tumor neovasculature, using in vivo video microscopy. Female C57BL/6 mice bearing 20 mm2 MC38 or LLC tumors that are TNF sensitive and resistant tumors, respectively, had dorsal skinfold chambers implanted. The rate of interstitial accumulation of Texas red fluorescently labeled albumin in tumor and normal vasculature was measured after intravenous TNF, cAu‐TNF or PBS. Changes in interstitial fluorescent intensity over time were quantified as a reflection of alterations in vascular permeability. MC38 bearing mice treated with TNF or cAu‐TNF demonstrated a rapid, selective and significant increase in tracer accumulation in areas of neovasculature compared to those of normal vasculature. Experiments in LLC tumor bearing mice showed similar results. Monoclonal antibody against tissue factor partially abrogated the effects of TNF on MC38 neovasculature. These data provide direct evidence that TNF and cAu‐TNF selectively and rapidly alter permeability in tumor neovasculature; a phenomenon that may be exploited to enhance selective delivery of chemotherapeutics to tumor. © 2007 Wiley‐Liss, Inc.
The differentiation antigen NY‐BR‐1 is a potential target for antibody‐based therapies in breast cancer
Authors: Seil Inka , Frei Claudia , Sültmann Holger , Knauer Shirley K. , Engels Knut , Jäger Elke , Zatloukal Kurt , Pfreundschuh Michael , Knuth Alexander , Tseng‐Chen Yao , Jungbluth Achim A. , Stauber Roland H. , Jäger Dirk 
Published: February 2007

AbstractAntibody‐based cancer immunotherapy relies on the identification and characterization of target antigens and the development of potent antibodies recognizing the target. Here we report the expression analysis and molecular characterization of the differentiation antigen NY‐BR‐1, which we previously identified by using the SEREX (serological analysis of recombinant cDNA expression libraries) method. Corroborating methodologies, including mRNA quantitation and immunoblotting show that NY‐BR‐1 is strongly expressed in >70% of 129 breast tumors. Application of a NY‐BR‐1 specific antibody demonstrated NY‐BR‐1 expression in primary and metastastic breast cancers. In contrast, most of the breast cancer cell lines tested, expressed only low NY‐BR‐1 levels. Importantly, confocal microscopy revealed that ectopically expressed NY‐BR‐1 localizes to the cytoplasm and the cell membrane. NY‐BR‐1 localization in breast cancer specimens was also confirmed by immunohistochemistry. Bioinformatic analysis and deletion mutagenesis further show that NY‐BR‐1 membrane localization is mediated by 2 cis‐active membrane targeting domains. Biochemical surface labeling and FACS analysis of live cells further characterize NY‐BR‐1 as a transmembrane protein, which can be specifically recognized by the anti‐NY‐BR‐1 antibody on the surface of vital cells. The strong expression of NY‐BR‐1 in breast tumors, its cytoplasmic and membrane localization and accessibility to an ectopically applied antibody now suggest to pursue NY‐BR‐1 as a potential target for antibody‐based therapies in breast cancer patients. © 2007 Wiley‐Liss, Inc.
Comparison of oxaliplatin‐ and curcumin‐mediated antiproliferative effects in colorectal cell lines
Authors: Howells Lynne M. , Mitra Anita , Manson Margaret M. 
Published: February 2007

AbstractColorectal cancer remains a leading cause of cancer death worldwide, despite markedly improved response rates to current systemic therapies. Oxaliplatin either alone or incorporated into 5‐fluorouracil/leucovorin regimes has resulted in increased survival rates, particularly with regards to metastatic colorectal carcinoma. The chemopreventive polyphenol curcumin, which is currently in clinical trial, has been advocated for use in colorectal cancer either singly or in combination with chemotherapeutic drugs. In this study, the antiproliferative capacity of both compounds was compared in HCEC (normal‐derived), HT29 (p53 mutant adenocarcinoma) and HCT116 (p53wt adenocarcinoma) colorectal cell lines to determine whether effects were cell‐type specific at pharmacologically achievable doses, and whether the combination resulted in enhanced efficacy. Both oxaliplatin and curcumin displayed marked antiproliferative capacity at therapeutic concentrations in the two tumor cell lines. Order of sensitivity to oxaliplatin was HCT116>HT29>HCEC, whereas order of sensitivity to curcumin was HT29>HCT116>HCEC. HCT116 cells underwent induction of G2/M arrest in response to both oxaliplatin (irreversible) and curcumin (reversible). Apoptosis was induced by both agents, and up to 16‐fold induction of p53 protein was observed in response to the combination. Antiproliferative effects in HT29 cells were largely cell cycle independent, and were mediated by induction of apoptosis. Effects were greatly enhanced in both cell lines when agents were combined. This study provides further evidence that curcumin may be of use in therapeutic regimes directed against colorectal cancer, and suggests that in combination with oxaliplatin it may enhance efficacy of the latter in both p53wt and p53 mutant colorectal tumors. © 2007 Wiley‐Liss, Inc.