bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copper Nanoparticles and Copper-Containing Metal–Organic Coordination Polymers in the Catalytic Amination of 2-Halopyridines
Authors: Leksakov D. A. , Borisova A. S. , Murashkina A. V. , Kuliukhina D. S. , Averin A. D. , Vergun V. V. , Isaeva V. I. , Savelyev E. N. , Novakov I. A. , Beletskaya I. P. 
Published: February 2025

Abstract
The efficiencies of copper-containing metal–organic coordination polymers (Cu-MOFs) and com­mercially available unsupported copper nanoparticles (CuNPs) in the amination of 2-iodopyridine, 2-bromo­pyridine, 2-bromo-5-(trifluoromethyl)pyridine, and 2-bromo-6-(trifluoromethyl)pyridine with n-octylamine and adamantane-containing amines with different steric environments of the amino group have been compared. Under the optimized conditions, the yields of the amination products in the presence of both catalytic systems were generally similar. In the reactions with 2-bromopyridine, increased reactant concentration and the use of 2 equiv of the substrate were necessary to achieve good yields. The presence of a trifluoromethyl group at the 6-position of the pyridine ring improved the yield of the amination products. An increase in steric hindrances at the amino group resulted in an appreciable decrease in the yield under catalysis by Cu-MOFs; however, reactions with such amines were successfully performed in the presence of CuNPs, and CuNPs of the average size 25 nm proved to be superior to bifractional nanoparticles with average sizes of 10 and 80 nm. On the other hand, increased concentration of the reactants in the Cu-MOF-catalyzed amination provided good yields of the target compounds in the absence of a ligand.
Synthesis of 4,13-Dioxo-3,14-dioxa-5,12-diazahexadecane-1,16-diyl Bis[3(4)-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoates]
Authors: Kolyamshin O. A. , Mitrasov Yu. N. , Danilov V. A. 
Published: February 2025

Abstract
New bis-carbamates, 4,13-dioxo-3,14-dioxa-5,12-diazahexadecane-1,16-diyl bis[3(4)-aminoben­zoates], were synthesized by the reaction of bis(2-chloroethyl) hexane-1,6-diyldicarbamate with potassium 3- and 4-aminobenzoates. These compounds reacted with maleic anhydride at a ratio of 1:2 to give the corre­sponding bis-maleamic acids which were cyclized in acidic medium to afford 4,13-dioxo-3,14-dioxa-5,12diaza­hexa­decane-1,16-diyl bis[3(4)-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoates]. Physical and spectral properties of the obtained bis-maleimides were studied.
Improving quality and outcomes of extracorporeal cardiopulmonary resuscitation in refractory cardiac arrest: the Phoenix ECPR project
Authors: Akhtar Waqas , Galiatsou Eftychia , Pinto Sofia , Comanici Maria , Gerlando Emanuele , Pitt Timothy , Hughes Joe , Maunz Olaf , Keating Elia , Taylor Michael , McKay Anthony , Gangata Julia , Thiruthalil Sumesh , Ross Eleanor , Avetoom Sophie , Durrant Jane , Smith Jill , Collins Ciara , Akhtar Majid , Jothidasan Anand , Monteagudo-Vela Maria , Mason Mark , McGovern Ian , Mitchell Jerry , Aboumarie Hatem Soliman , Kviatkovske Orinta , Bullen Caroline , Mehta Sachin , Kaul Sundeep , Hall Donna , Hernandez Caballero Clara , Hurtado Ana , Lees Nick , Panoulas Vasileios , Rosenberg Alex 
Published: February 2025

BackgroundSurvival for cardiac arrest remains poor, and the use of extracorporeal cardiopulmonary resuscitation (ECPR) has been suggested as a potential therapy to improve outcomes. Harefield Hospital has been performing ECPR for over a decade; however outcomes have been historically poor.MethodsA retrospective analysis was performed of all ECPR cases at Harefield Hospital between April 2018 and April 2023. A new structured system including a systematic screening process, strict exclusion criteria, assessment of resuscitation adequacy through physiological stop criteria, drilled and standardised cannulation process, post-resuscitation care and neuroprognostication was rolled out on 1 April 2023. A comparison of ECPR performed pre and post was undertaken.ResultsWith the institution of the new system, 13 patients were treated with ECPR over 1 year; of which nine (69.2%) survived with good neurological outcomes at 6 months. In the preceding 3 years, there had been 22 cases; of which, two survived (9.1%). This was a statistically significant higher survival with HR of 4.56 (CI 2.1, 10.2, p<0.05).The average length of hospital stay in the new system was 61.3 days; of which, 32.4 days were in the intensive care unit.ConclusionsThis study shows that it is possible to significantly improve outcomes in ECPR in a single centre by introducing into standard practice, a high-quality bundle of care.
An observational, multigenerational platform for health systems and population health interventions to minimise intergenerational transmission of non-communicable diseases in India: the YUVAAN cohort study protocol
Authors: Miriam Demi , Mandlik Rubina , Patwardhan Vivek , Ladkat Dipali , Khadilkar Vaman , Kajale Neha , More Chidvilas , Gondhalekar Ketan , Bhawra Jasmin , Katapally Tarun Reddy , Khadilkar Anuradha Vaman 
Published: February 2025

IntroductionNon-communicable diseases (NCDs) pose a significant health burden in India, with preventable risk factors contributing to their prevalence. In rural populations, inequalities may be reinforced by health risks passed down through generations. Taking a life course perspective, this multigenerational cohort study aims to investigate behavioural, socioecological, and socioeconomic determinants of growth and NCD risk, as well as healthcare access and utilisation among preadolescents and their parents.Methods and analysisThe study is being implemented by the Hirabai Cowasji Jehangir Medical Research Institute (HCJMRI) using a prospective, multigenerational cohort design to investigate NCD risk over 15 years. Data are being collected from 14 villages around Pune, Maharashtra, India. The target population is asymptomatic (ie, healthy) children aged 8–10 years and their parents. The study commenced on 13 September 2022. Participants (children and their parents) are being enrolled through household visits, and by arranging subsequent visits to the primary health facility of HCJMRI. After obtaining informed consent from participants (parents and their children), comprehensive data are being collected from both children and parents, including clinical, behavioural, healthcare access and utilisation, as well as socioeconomic determinants of health. Clinical assessments include anthropometric measurements, blood samples for a wide range of NCD indicators, bone health and muscle function. The long-term data analysis plan includes longitudinal modelling, time-series analyses, structural equation modelling, multilevel modelling and sex-based analyses to investigate growth trajectories and intergenerational patterns of health risks. As of November 2024, 1070 families from 14 villages have been enrolled (1264 preadolescents and 2140 parents). Given the double burden of malnutrition, with undernutrition and overweight/obesity coexisting among children and parents in India, the study findings will contribute to the development of focused interventions aimed at lowering NCDs, addressing the generational transmission of health risks, and improving health outcomes for rural communities.Ethics and disseminationEthics approval was obtained from the institutional ethics committee, Ethics Committee Jehangir Clinical Development Centre, No: JCDC/BHR/24/047.Trial registration numberNCT05603793.
Symptomatic vitamin A deficiency in a patient on long-term colesevelam
Authors: Andreyev Daniel Alexei , Gopakumar Veena , Andreyev H Jervoise N 
Published: February 2025

IntroductionBile acid sequestrants including colesevelam are increasingly used by gastroenterologists. The risk of fat-soluble vitamin deficiency when sequestrants are used is recognised. Vitamin A deficiency is uncommon in the UK, but delayed diagnosis can cause irreversible blindness. There are no guidelines for the monitoring of fat-soluble vitamin levels.Case discussionWe describe a 63-year-old man who had a neuroendocrine tumour, treated with ileal resection and lanreotide and complicated by the development of severe bile acid malabsorption, pancreatic exocrine insufficiency and intermittent episodes of small intestinal bacterial overgrowth. Three and a half years after starting colesevelam, he developed progressive visual symptoms due to severe vitamin A deficiency. After diagnosis and parenteral vitamin A replacement, his visual symptoms improved but have not resolved.ConclusionsClinicians prescribing bile acid sequestrants should be aware that vitamin A deficiency may arise and should consider regular monitoring of serum levels.
TheICAM1p.K56M variant and risk of heart failure in chronic kidney disease: the Chronic Renal Insufficiency Cohort study
Authors: Culler Kasen , Zelnick Leila R , Mehta Rupal C , de Sosa Giselle R , Kansal Mayank , Rao Panduranga S , Bhat Zeenat , Taliercio Jonathan , Horwitz Edward , Chen Jing , He Jiang , Shah Sanjiv J , Isakova Tamara , Patel Ravi B 
Published: February 2025

BackgroundIntercellular adhesion molecule-1 (ICAM-1) is a cell-surface protein that facilitates inflammation through leucocyte adhesion. Approximately 35% of Black individuals carry at least one copy of anICAM1missense variant (rs5491; p.K56M), which has been associated with increased risk of heart failure (HF) with preserved ejection fraction (HFpEF). Whether the risk of HFpEF conferred by rs5491 extends to individuals with chronic kidney disease (CKD), a cohort at high risk for HF, is unknown.AimsWe investigated the association between rs5491 and the incidence of HF in CKD.MethodsWe estimated associations of rs5491 with incident HF and HF subtypes among Black individuals who were free from HF at baseline in the Chronic Renal Insufficiency Cohort (CRIC). The CRIC study recruited individuals who were 21–74 years old with estimated glomerular filtration rate (eGFR) of 20–70 mL/min/1.73 m2at baseline.ResultsAmong 1267 Black participants (mean age 57±11 years, 51% female, mean eGFR 45±15 mL/min/1.73 m2), 464 (37%) had at least one copy of rs5491. During a median follow-up of 10.3 years (IQR: 4.7–15.0 years), there were 309 incident overall HF hospitalisations (160 HFpEF, 111 HF with reduced ejection fraction (HFrEF) and 38 HF with unknown ejection fraction). Each additional rs5491 allele was significantly associated with incident HFpEF (HR 1.35, 95% CI 1.03 to 1.76, p=0.029). The rs5491 variant was not associated with incident overall HF (HR 1.14, 95% CI 0.93 to 1.39, p=0.20) or incident HFrEF (HR 0.76, 95% CI 0.53 to 1.10, p=0.15).ConclusionTheICAM1p.K56M variant is specifically associated with increased risk of incident HFpEF among individuals with CKD.
miRNA-214 to predict progression and survival in ALS
Authors: Noh Min-Young , Kwon Min-Soo , Oh Ki-wook , Nahm Minyeop , Park Jinseok , Jin Hee Kyung , Bae Jae-sung , Son Bugyeong , Kim Seung Hyun 
Published: February 2025

BackgroundReliable biomarkers are essential for predicting the progression speed and prognosis of patients with amyotrophic lateral sclerosis (ALS). We previously identified NCK-associated protein 1 (NCKAP1) as a critical factor in the defective phagocytosis observed in induced microglia-like cells (iMGs) from patients with rapidly progressive sporadic ALS. This study explored the roles of microRNA (miRNA)-214, which targets theNCKAP1gene, in the progression of ALS.MethodsThe discovery cohort (n=29) was used to identify miR-214 targetingNCKAP1genes. The validation cohort (n=132) was used to determine the clinical usability of miR-214 for predicting disease progression speed and survival time.ResultsIn the discovery cohort, miR-214 levels were increased in plasma and iMGs from rapidly progressive ALS participants. This finding was validated in another cohort of 132 ALS participants and 30 age-matched healthy volunteers. Plasma miR-214 levels correlated with disease progression, severity and survival, distinguishing between rapidly progressive and slowly progressive ALS. In addition, miR-214 levels also correlated with plasma neurofilament light chain (NfL) and cerebrospinal fluid inflammatory cytokines, showing specific associations with increased NfL and monocyte chemoattractant protein 1 (MCP-1). Survival prediction accuracy improved when miR-214 levels were considered with NfL or MCP-1 levels.ConclusionsPlasma miRNA-214 could serve as a novel biomarker for predicting the progression and prognosis of ALS.
How do clinicians address vaping in an illegal context? A qualitative Singapore study
Authors: van der Eijk Yvette , Kwok Wei Yi , Tan Grace Ping Ping 
Published: February 2025

BackgroundDespite a strict vaping ban also covering purchase, use and possession, vaping has become more prevalent in Singapore. As more countries seek to regulate vaping, with no tailored services or guidelines for vaping cessation, clinicians may face additional challenges in identifying or treating vaping cases due to stigma or patients’ reluctance to disclose their vaping. Few studies have explored how clinicians identify or manage vaping cases in a context where vaping is heavily regulated or stigmatised.MethodsWe conducted semistructured interviews with 12 Singapore practitioners from pharmacy, psychology, respiratory medicine, smoking cessation or youth welfare settings, all of whom had encountered vaping cases in their practice. Interviews explored how they manage vaping cases. We analysed data using inductive thematic methods.ResultsPatients were described as reluctant to disclose their vaping for fear of being reported to authorities, and some clinicians were unsure of their duty to report, making it challenging to identify vaping cases. Variability in usage patterns and inaccurate e-liquid labelling posed difficulties in estimating nicotine dependence, leaving practitioners to trial and error or to adapt from smoking cessation guidelines. Tailored quit support for people who vape was lacking, especially in the form of subsidised nicotine replacement therapy.ConclusionEven in contexts where vaping is heavily regulated, given the rising incidence of vaping globally, it is important to record patients’ vaping history as part of routine practice and to provide services to help people quit vaping without facing stigma or legal repercussions because of their vaping.
Associations between parity, history of breastfeeding, and T cell profile of ovarian tumors
Authors: Mongiovi Jennifer M. , Townsend Mary K. , Vitonis Allison F. , Harris Holly R. , Doherty Jennifer A. , Babic Ana , Hecht Jonathan L. , Soong T  Rinda , Titus Linda , Conejo-Garcia Jose R. , Fridley Brooke L. , Tworoger Shelley S. , Terry Kathryn L. , Sasamoto Naoko 
Published: February 2025

Abstract
Background: Parity and breastfeeding are associated with systemic changes in maternal inflammation and reduced risk of ovarian cancer, but little is known about their impact on the ovarian tumor immune microenvironment. Methods: We evaluated the associations of self-reported parity and history of breastfeeding with tumor-infiltrating T cells among 1,706 ovarian carcinoma cases with tumor tissue collected across four studies. The abundance of tumor-infiltrating T cells was measured by multiplex immunofluorescence in tumor tissue microarrays. Odds ratios (OR) and 95% confidence intervals (CIs) for the positivity of tumor immune cells were calculated using beta-binomial models and stratified by histotype. Results: Compared to ovarian tumors in nulliparous women, there was no association between parity and ovarian tumor T cell abundance among all histotypes combined, but suggestion of increased cytotoxic T cells and T cell exhaustion among parous women with clear cell tumors. When restricted to parous women, history of breastfeeding was associated with increased odds for all T cell types (i.e., total, cytotoxic, helper, regulatory, and exhausted T cells), with ORs ranging from 1.11-1.42. For every 6 months of breastfeeding, we observed increased odds of activated helper T cell infiltration (CD3+CD4+CD69+, OR:1.13, 95% CI: 0.99-1.29), with a similar association for high-grade serous tumors, but lower odds in clear cell tumors (OR:0.43, 95% CI:0.21-0.87). Conclusions: History of breastfeeding may alter the ovarian tumor immune microenvironment by modulating the abundance of tumor-infiltrating T cells. Impact: While replication is required, history of breastfeeding may play a role in activation of the ovarian tumor immune response.
Temperature and Maltreatment of Young Children
Authors: Evans Mary F. , Gazze Ludovica , Schaller Jessamyn 
Published: February 2025

Abstract
We estimate the impacts of temperature on alleged and substantiated child maltreatment among young children using administrative data from state child protective services agencies. Leveraging short-term weather variation, we find increases in the number of young children involved in cases of alleged and substantiated maltreatment during hot periods. Additional analysis identifies neglect as the temperature-sensitive maltreatment type, and we find some evidence that adaptation via air conditioning mitigates this relationship. Given that climate change will increase exposure to extreme temperatures, our findings speak to additional costs of climate change among the most vulnerable.
Cholera, the Ulama, and Public Health in Russia and the Kazakh Steppe, 1830–1908
Authors: Frank Allen J. 
Published: February 2025

Abstract
The recurring cholera epidemics in 19th- and early 20th-century Russia and the Kazakh Steppe required the Russian government to mobilize the religious authority of the ulama to disseminate and implement public health measures among the region’s Muslim population. Muftis and legal scholars issued a series of fatwas and treatises calling on Muslims to adhere to public health directives, and to educate Muslims more broadly about the etiology and characteristics of cholera. To do so, they invoked legal and scholarly texts as the foundation for their authority, and for the authority of the directives. At the same time, they encouraged Muslim engagement with medical knowledge derived from non-Muslims by distinguishing medical science from Islamic religious science and describing it as a humanistic universal type of knowledge. The widespread acceptance of this stance brings into question stereotypes of Muslim resistance to “European” medicine.
In the eye of the sufferer: The impact of perceived motivation and expectations for compassion on sufferer’s emotional engagement
Authors: Wei Hongguo , Kanov Jason 
Published: February 2025

Compassion—an empathically motivated behavioral response to suffering that has been linked to numerous organizational benefits—is understood to be a relational process, but our understanding of the sufferer’s role in how that process plays out and to what effect is lacking due to limited attention to this role in the extant compassion literature. This hinders our knowledge of compassion as well as our understanding of how to promote workplace compassion. To address this, we conducted two studies examining how sufferers’ perceptions of compassion givers’ motives (other-oriented and self-oriented, which may co-occur) and their own expectations for compassion affect how they experience compassion. Results show that while the impact of compassion on sufferers’ emotional engagement was enhanced when they viewed compassion givers as more other-oriented and weakened when they viewed them as more self-oriented, these relationships were nuanced and also moderated by sufferers’ expectations. In particular, when sufferers saw compassion givers as more self-oriented, this only lessened the positive impact of compassion for sufferers when they concurrently perceived low other-oriented motives; this effect went away when they perceived other-oriented motives as concurrently high. A three-way interaction was also identified such that sufferers benefited from compassion more when they had high expectations for compassion and saw compassion givers as highly other-oriented regardless of how self-oriented compassion givers concurrently seemed to be. We discuss the results, implications, and limitations. JEL Classification: M14
Complete genome sequencing and probiotic characterization of promising lactic acid bacterial strains isolated from dairy products in Egyptian markets
Authors: El-Hosseny Mostafa F. , Seadawy Mohamed G , Abdel-Monem Mohammed O , Hassan Mervat G 
Published: February 2025

Abstract

Background
Probiotics refer to live bacteria that, when administered in a sufficiently, exert a beneficial influence on human health. Due to the probiotics' beneficial health advantages, dietary supplements are expanding rapidly as a self-care interest worldwide. It may be beneficial to administer probiotic strains resistant to antibiotics concurrently with an antibiotic treatment. Our study investigates nineteen dairy products collected from Egyptian markets, isolated, identified and underwent a characterization for probiotic features under demanding circumstances as NaCl, acid and bile salt environments. The antibiotic sensitivity test was performed later to the antimicrobial assessment against widespread both negative and positive gram-stained bacteria infecting humans, along with the antiviral evaluation against (SARS-CoV-2), the virus that has disturbed the world recently.

Results
Out of nineteen investigated isolates, five potential probiotic isolates were examined for probiotic characteristics. Our tested samples were of dairy origin (yogurt, cottage-cheese and sour milk) in Egypt, were identified as Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus and Pediococcus acidilactici. These promising isolates had withstood stressful factors, such as NaCl, acid, bile salts, and the antimicrobial advance. The genomes were characterized for the physiology, safety, and efficacy of these isolates for probiotic qualities plus the presence of mobile genetic components and prophages that influence the genome's flexibility. They lack virulence factors and pathogenicity, rather than the lack of antibiotic resistance genes.

Conclusion
Three promising isolates underwent complete genome sequencing with high-throughput second generation technology followed by comprehensive bioinformatic analysis. The results showed that our isolates possess traits enabling resilience to antimicrobial effects and stress factors that might cause problems in the human gut. Several trustworthy genomic analysis methods were used to confirm and provide detailed illustrations of all traits. Genomic analyses confirmed the presence of stable genomes due to including mobile genetic components such as phages and CRISPR clusters, which validate their quality and safe usage for human health.