bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemico-pharmacological evaluation of the methanolic leaf extract of Catharanthus ovalis: GC–MS/MS, in vivo, in vitro, and in silico approaches
Authors: Shahriar Saimon , Shermin Samia Akter , Hasnat Hasin , Hossain Faisal , Han Aixia , Geng Peiwu , Alam Safaet , Mamun Abdullah Al 
Published: March 2024

Introduction: Natural plant-based medicines have gained popularity recently as a major source of inventive, risk-free, and more potent secondary bioactive compounds with medicinal potential. Catharanthus ovalis is a perennial shrub containing various indole alkaloids cultivated extensively for local medical purposes.Methods: This research is conducted to identify the phytocompounds present in the leaves of C. ovalis and its central and peripheral analgesic, thrombolytic, and membrane-stabilizing activities through tail immersion, acetic acid-induced writhing, human blood clot lysis, and erythrocyte lysis by heat and hypotonic solution methods, respectively.Results and discussion: A total of 39 compounds were identified using GC–MS/MS techniques, including hexadecanoic acid, methyl ester (56.749%), methyl stearate (29.782%), carvacrol and its TBDMS derivative (12.586%), and 9-octadecenoic acid, methyl ester, (E)-] (9.297%) presented in high quantity. The highest tail immersion latency was observed for the 600 mg/kg extract of C. ovalis crude extract. Both 400 and 600 mg/kg doses of C. ovalis crude extract exhibited prominent peripheral analgesic activity. The maximum thrombolytic effect was observed by DCM soluble fraction extract by inhibiting 54.87% of the clot. However, the aqueous-soluble fraction of this extract manifested an excellent membrane-stabilizing effect by showing 73.98% and 87.51% hemolysis against heat- and hypotonic-induced hemolysis, respectively. Some of the compounds were identified as active agents against different receptors related to these diseases, which supported the findings of in vitro and in vivo tests.Conclusion: Further investigation needs to be conducted to specify and identify the exact mechanism of action of these compounds.
Correlations of religious beliefs with anxiety and depression of Chinese adolescents
Authors: Li Lejun , Liu Xiliang , Wang Pingping , Qu Miao , Xiu Meihong 
Published: March 2024

ObjectiveThis study was designed to investigate the prevalence of religious belief and its relationship with psychiatric symptoms among Chinese adolescents.MethodsThis study recruited 11,603 adolescents in Grades 7-9 from March 21 to 31, 2020 in five cities in China. The religious beliefs of adolescents were collected by asking whether they held religious beliefs and what type of religious beliefs they held. The Patient Health Questionnaire-9 (PHQ-9) and the Generalized Anxiety Disorder-7 Scale (GAD-7) were used to assess depressive and anxiety symptoms in all adolescents. Demographics, religious beliefs, and mental health status were collected through the professional version of Wenjuanxing.ResultsOf 11,069 valid questionnaires collected, 847 (7.7%) reported holding religious beliefs. Adolescents with religious beliefs showed significantly more severe symptoms of depression and anxiety compared to those without religious beliefs (both p&lt;0.05). Logistic regression analysis revealed that religious belief was a risk factor for symptoms of depression (OR = 1.37, 95%CI: 1.16-1.61, p &lt; 0.001) and anxiety (OR = 1.49, 95%CI: 1.23-1.79, p &lt; 0.001) after controlling age, gender, and parental marital status.ConclusionsOur findings suggest that religiousness in adolescents was associated with a higher likelihood of depression/more intense depressive symptoms. In addition, religious Chinese adolescents should be provided with more resources to help them cope with mental health concerns.
Staff perspectives on poor mental health in secondary school students: an increasing problem handled with insufficient resources
Authors: Svensson Åsa , Warne Maria 
Published: March 2024

IntroductionAn increasing number of schools are recognizing the importance of addressing students' mental health based on the association with educational outcomes and long-term health. The school organization and the members of the school staff play important but, in several ways, challenging roles in this work. The purpose of this study was to explore views of staff from schools and school health services on mental ill health among students and their own role in detecting and managing it.MethodsA qualitative study was conducted in a sparsely populated municipality in northern Sweden. In total, 40 participants from three secondary schools and the school health services participated either in focus groups or individual interviews. Participants were teachers, assistants, school nurses, school counselors and psychologists. Data were analyzed using thematic analysis.ResultsThe analysis revealed the main theme Student mental ill health: an increasing problem handled with insufficient resources and two subthemes, i.e., Uncertainty in interpreting students' signs of mental ill health and the need to clarify roles and establish a supportive organization.ConclusionsIt was concluded that school staff were uncertain regarding how to interpret signs of mental ill health among students and required better knowledge and more resources to help students with mental ill health. A clearer organization and consensus regarding support for students with mental ill health were also necessary in light of the division of responsibilities between school staff and the school health services.
Predictors of adherence in Austrian employees during the COVID-19 pandemic: results of an online survey
Authors: Avian Alexander , Könczöl Clemens , Kubicek Bettina , Spary-Kainz Ulrike , Siebenhofer Andrea 
Published: March 2024

BackgroundSince the beginning of the pandemic in December 2019, Coronavirus disease 2019 (COVID-19) has been a significant challenge to health care systems throughout the world. The introduction of measures to reduce the incidence of infection had a significant impact on the workplace. Overall, companies played a key and adaptive role in coping with the pandemic.MethodsCross-sectional data from an online-survey of 1,183 employees conducted during the COVID-19 pandemic in spring 2021 in Austria were used in the analyses. The influence of health beliefs (e.g., perceived severity), modifying factors (e.g., age) and time-dependent factors (e.g., corona fatigue) on individual adherence were evaluated. The conception of the questionnaire was based on the health belief model.ResultsThe majority of respondents were female (58.3%), worked in companies with more than 250 employees (56.6%) and had been to an academic secondary school or had a university degree (58.3%). Overall, employees were adherent to most of the measures at their company (&gt;80%), except for wearing FFP-2 masks when they were travelling in a car with coworkers (59.3, 95%CI 51.3–66.7%). Overall adherence was associated with high ratings for the meaningfulness of testing (OR: 2.06 95%CI: 1.00–4.22; p = 0.049), the extent to which social norms govern behavior (OR: 6.61 95%CI: 4.66–9.36; p &lt; 0.001), lower perceived difficulties associated with the adoption of health-promoting measures (OR: 0.37 95%CI: 0.16–0.82; p = 0.015) and lower corona fatigue (OR: 0.23 95%CI: 0.10–0.52; p &lt; 0.001). Adherence to four single measures was influenced by different predictors. The most important predictors (important for the adherence to three out of four single measures) were social norms and corona fatigue.ConclusionThe importance attached to testing and social norms, as well as lower perceived barriers to health-promoting measures and low levels of corona fatigue all increase overall adherence to Covid-19 protective measures in companies. Strategies to improve adherence should be adapted depending on the aim (to raise overall adherence or adherence to individual measures) and on the group of persons that is being targeted.
Assessing electrogenetic activation via a network model of biological signal propagation
Authors: Chun Kayla , VanArsdale Eric , May Elebeoba , Payne Gregory F. , Bentley William E. 
Published: March 2024

Introduction:Molecular communication is the transfer of information encoded by molecular structure and activity. We examine molecular communication within bacterial consortia as cells with diverse biosynthetic capabilities can be assembled for enhanced function. Their coordination, both in terms of engineered genetic circuits within individual cells as well as their population-scale functions, is needed to ensure robust performance. We have suggested that “electrogenetics,” the use of electronics to activate specific genetic circuits, is a means by which electronic devices can mediate molecular communication, ultimately enabling programmable control.Methods:Here, we have developed a graphical network model for dynamically assessing electronic and molecular signal propagation schemes wherein nodes represent individual cells, and their edges represent communication channels by which signaling molecules are transferred. We utilize graph properties such as edge dynamics and graph topology to interrogate the signaling dynamics of specific engineered bacterial consortia.Results:We were able to recapitulate previous experimental systems with our model. In addition, we found that networks with more distinct subpopulations (high network modularity) propagated signals more slowly than randomized networks, while strategic arrangement of subpopulations with respect to the inducer source (an electrode) can increase signal output and outperform otherwise homogeneous networks.Discussion:We developed this model to better understand our previous experimental results, but also to enable future designs wherein subpopulation composition, genetic circuits, and spatial configurations can be varied to tune performance. We suggest that this work may provide insight into the signaling which occurs in synthetically assembled systems as well as native microbial communities.
Benefits of heat-killed Lactobacillus acidophilus on growth performance, nutrient digestibility, antioxidant status, immunity, and cecal microbiota of rabbits
Authors: Xia Miao , LI Chong , Wu Diange , Wu Fengyang , Kong Lingchang , Jia Zifan , Han Wenxiao , Chen Saijuan , Fang Wei , Liu Yajuan , Chen Baojiang 
Published: March 2024

IntroductionHeat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits.MethodsTwo hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively.ResultsResults revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p &lt; 0.05). Additionally, the L800 group exhibited significantly higher apparent digestibility of crude fiber (CF) and crude protein (CP) (p &lt; 0.05). Regarding digestive enzyme activities, enhanced trypsin and fibrinase activities were observed in the L600 and L800 groups (p &lt; 0.05). Concerning the regulation of the body’s antioxidant status, the L800 group demonstrated elevated levels of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in both serum and ileal tissue (p &lt; 0.05). In terms of immune capacity modulation, serum tumor necrosis factor-α (TNF-α) levels were significantly lower in the L600 and L800 groups (p &lt; 0.05), while immunoglobulin A (IgA) and immunoglobulin M (IgM) levels were higher (p &lt; 0.05). Additionally, the L800 group exhibited a substantial increase in secretory immunoglobulin A (SIgA) levels in the intestinal mucosa (p &lt; 0.05). In comparison to the CON group, the L800 group exhibited a significant increase in the relative abundance of Phascolarctobacterium and Alistipes in the cecum (p &lt; 0.05). Phascolarctobacterium demonstrated a positive correlation with SIgA (p &lt; 0.05), IgM (p &lt; 0.01), and Glutathione peroxidase (GSH-Px) (p &lt; 0.05), while displaying a negative correlation with TNF-α levels (p &lt; 0.05). Concurrently, Alistipes exhibited positive correlations with IgA (p &lt; 0.05), IgM (p &lt; 0.05), SIgA (p &lt; 0.01), GSH-Px (p &lt; 0.05), SOD (p &lt; 0.05), and T-AOC (p &lt; 0.01), and a negative correlation with TNF-α (p &lt; 0.05).DiscussionIn conclusion, the dietary incorporation of 600 mg/kg and 800 mg/kg of heat-killed LA positively influenced the growth performance, nutrient digestibility, antioxidant status, immune capacity and cecal microbiota of rabbits. This highlights the potential benefits of utilizing heat-killed probiotics in animal nutrition.
Effects of Harvesting Method on Seed Yield and Seed Quality in Urochloa ruziziensis (cv. ‘OKI-1’ and cv. ‘Br-203’)
Authors: Imura Yoshimi , Nakamura Ichiro , Juntasin Weenaporn , Hossain Mohammad Amzad , Thaikua Sarayut , Poungkaew Rattikan , Kawamoto Yasuhiro 
Published: March 2024

Two field trials were conducted in Northern Thailand from 2018 to 2020 to determine the best manual harvesting method for maximizing seed yield and seed quality of two Urochloa ruziziensis cultivars, cv. OKI-1 and cv. Br-203. Four manual seed harvesting methods were implicated in a randomized complete block design with four replicates. The four methods were as follows: knocking seeds from seedheads into a nylon net receptacle and collecting them once every day (T1); knocking seeds from seedheads into a nylon net receptacle and collecting them once every three days (T2); allowing ripe seeds to fall into a nylon net sheet that was stretched as a receptacle beneath the seedheads and collecting seeds once every five days (T3); and covering the tied seedheads with a nylon net bag and collecting seeds once every five days (T4). The highest pure seed yield was obtained from T3 in cv. Br-203 (333.11 kg/ha), as well as the highest trend in cv. OKI-1 (534.67 kg/ha). T3 produced 22–46% and 11–27% more pure germinated seed yields than the other methods in cv. Br-203 and cv. OKI-1, respectively. As a result, T3 was recommended for higher seed yield, convenient seed harvest, and reduced manual seed harvest time.
Transcriptome in Liver of Periparturient Dairy Cows Differs between Supplementation of Rumen-Protected Niacin and Rumen-Protected Nicotinamide
Authors: Zhang Yuanjie , Li Rongrong , Du Xue’er , Cui Zhijie , Jiang Xingwei , Wang Lamei , Yao Junhu , Liu Shimin , Wang Jianguo , Cai Chuanjiang , Cao Yangchun 
Published: March 2024

To investigate the difference between rumen-protected niacin (RPN) and rumen-protected nicotinamide (RPM) in the transcriptome of genes relating to the lipid metabolism of the liver of periparturient dairy cows, 10 healthy Chinese Holstein cows were randomly divided into two groups and fed diets supplemented with 18.4 g/d RPN or 18.7 g/d RPM, respectively. The experiment lasted from 14 days before to 21 days after parturition. Liver biopsies were taken 21 days postpartum for transcriptomic sequencing. In addition, human LO2 cells were cultured in a medium containing 1.6 mmol/L of non-esterified fatty acids and 1 mmol/L niacin (NA) or 2 mmol/L nicotinamide (NAM) to verify the expression of the 10 genes selected from the transcriptomic analysis of the liver biopsies. The expression of a total of 9837 genes was detected in the liver biopsies, among which 1210 differentially expressed genes (DEGs) were identified, with 579 upregulated and 631 downregulated genes. These DEGs were associated mainly with lipid metabolism, oxidative stress, and some inflammatory pathways. Gene ontology (GO) enrichment analysis showed that 355 DEGs were enriched in 38 GO terms. The differences in the expression of these DEGs between RPN and RPM were predominantly related to the processes of steroid catabolism, steroid hydroxylase, monooxygenase activity, oxidoreductase activity, hemoglobin binding, and ferric iron binding, which are involved mainly in lipid anabolism and redox processes. The expressions of FADS2, SLC27A6, ARHGAP24, and THRSP in LO2 cells were significantly higher (p < 0.05) while the expressions of BCO2, MARS1, GARS1, S100A12, AGMO, and OSBPL11 were significantly lower (p < 0.05) on the NA treatment compared to the NAM treatment, indicating that NA played a role in liver metabolism by directly regulating fatty acid anabolism and transport, inflammatory factor expression, and oxidative stress; and NAM functioned more as a precursor of nicotinamide adenine dinucleotide (NAD, coenzyme I) and nicotinamide adenine dinucleotide phosphate (NADP, coenzyme II) to participate indirectly in biological processes such as ether lipid metabolism, cholesterol metabolism, energy metabolism, and other processes.
New Inhibitor Based on Hydrolyzed Keratin Peptides for Stainless Steel Corrosion in Physiological Serum: An Electrochemical and Thermodynamic Study
Authors: Samide Adriana , Iacobescu Gabriela Eugenia , Tutunaru Bogdan , Tigae Cristian , Spînu Cezar Ionuţ , Oprea Bogdan 
Published: March 2024

Reducing the impact of some biological fluids on bioimplants involves the control of surface characteristics by modeling the interface architecture and assembling ecofriendly thin films to retard corrosion. Therefore, a mixture of hydrolyzed keratin peptides (HKER) was investigated as a corrosion inhibitor for 304L stainless steel (SS) in physiological serum (PS), using electrochemical measurements associated with optical microscopy and atomic force microscopy (AFM). The tests, performed for various concentrations of the inhibitor at different temperatures, showed that the inhibition efficiency (IE) decreased with a rise in temperature and proportionally increased with the HKER concentration, reaching its maximum level, around 88%, at 25 °C, with a concentration of 40 g L−1 HKER in physiological serum. The experimental data best fitted the El-Awady adsorption model. The activation parameters (Ea, ∆Ha and ∆Sa) and the adsorption ones (∆Gads0, ∆Hads, ∆Sads) have highlighted a mixed action mechanism of HKER, revealing that physisorption prevails over chemisorption. AFM parameters, such as the average roughness (Ra), root-mean-square roughness (Rq) and maximum peak-to-valley height (Rp−v), confirmed HKER adsorption, indicating that a smoother surface of the 304L stainless steel was obtained when immersed in a PS-containing inhibitor, compared to the surface designed in blank solution, due to the development of a protective layer on the alloy surface.
First contact physiotherapy: an evaluation of clinical effectiveness and costs
Authors: Walsh Nicola E , Halls Serena , Thomas Rachel , Berry Alice , Liddiard Cathy , Cupples Margaret E , Gage Heather , Jackson Daniel , Cramp Fiona , Stott Hannah , Kersten Paula , Jagosh Justin , Foster Dave , Williams Peter 
Published: March 2024

BackgroundFirst contact physiotherapy practitioners (FCPPs) are embedded within general practice, providing expert assessment, diagnosis, and management plans for patients with musculoskeletal disorders (MSKDs), without the prior need for GP consultation.AimTo determine the clinical effectiveness and costs of FCPP models compared with GP-led models of care.Design and settingMultiple site case-study design of general practices in the UK.MethodGeneral practice sites were recruited representing the following three models: 1) GP-led care; 2) FCPPs who could not prescribe or inject (FCPPs-standard [St]); and 3) FCPPs who could prescribe and/or inject (FCPPs-additional qualifications [AQ]). Patient participants from each site completed outcome data at baseline, 3 months, and 6 months. The primary outcome was the SF-36 Physical Component Summary (PCS) score. Healthcare usage was collected for 6 months.ResultsIn total, 426 adults were recruited from 46 practices across the UK. Non-inferiority analysis showed no significant difference in physical function (SF-36 PCS) across all three arms at 6 months (P= 0.667). At 3 months, a significant difference in numbers improving was seen between arms: 54.7% (n= 47) GP consultees, 72.4% (n= 71) FCPP-St, and 66.4% (n= 101) FCPP-AQ (P= 0.037). No safety issues were identified. Following initial consultation, a greater proportion of patients received medication (including opioids) in the GP-led arm (44.7%,n= 42), compared with FCPP-St (18.4%,n= 21) and FCPP-AQ (24.7%,n= 40) (P<0.001). NHS costs (initial consultation and over 6-month follow-up) were significantly higher in the GP-led model (median £105.5 per patient) versus FCPP-St (£41.0 per patient) and FCPP-AQ (£44.0 per patient) (P<0.001).ConclusionFCPP-led models of care provide safe, clinically effective patient management, with cost-benefits and reduced opioid use in this cohort.
Penerapan Metode Analytic Hierarchy Process (AHP) Untuk Pemilihan Software Accounting
Authors: Wahyudin Wahyudin 
Published: March 2024

Software accounting merupakan fungsi sistem aplikasi software yang dapat membantu organisasi dalam mengendalikan bisnis yang lebih baik karena dapat mengurangi tingkat stok dan inventori, meningkatkan perputaran stok, mengurangi cycle time order, meningkatkan produktivitas, komunikasi lebih baik serta berdampak pada peningkatan benefit (profit) perusahaan. Aplikasi system pendukung keputusan yang dibangun dengan menggunakan metode AHP ini dapat membantu mempermudah dan mempercepat manajemen untuk menentukan software accounting. Dari hasil perhitungan pada table menunjukan bahwa kriteria Easy to user yang paling penting dalam pemilihan software accounting menjadi prioritas ke- 1 dengan nilai bobot 34%, berikutnya kriteria proteksi akan investasi menjadi prioritas ke- 2 dengan nilai bobot 22%, kemudian kriteria tingkat skalabilitas menjadi prioritas ke- 3 dengan nilai bobot 19%, kemudian kriteria mudah dicustomize menjadi prioritas ke- 4 dengan nilai bobot 14%, kemudian kriteria biaya menjadi prioritas ke-5 dengan bobot 10%. Accurate memiliki bobot prioritas tertinggi yaitu 0,452 atau 45,22%, Zahir memiliki bobot prioritas ketiga yaitu 0,243 atau 24,328%, Jurnal.id memiliki bobot prioritas kedua yaitu 0,316 atau 31,632%. Sehingga bisa disimpulkan Accurate yang akan digunakan dalam system operasional perusahaan.
Synthesis of Dipiperazine Thiuramdisulphide (DPTD) and its application as a safe accelerator for the vulcanisation of natural rubber
Authors: Kurian Thomas , Kozhikodanparambil Abhitha , Jabbar Shincy Alamparambil 
Published: March 2024

Dipiperazine thiuramdisulphide (DPTD) prepared in the laboratory using piperazine, triethyl amine and carbon disulphide as reactants acts as an accelerator for the vulcanization of natural rubber. The DPTD synthesised in our laboratory was characterized by FTIR, CHNS and the estimation of amine value. Results of the MTT assay test shows that the DPTD is a safe (non-carcinogenic) chemical. For comparable dosages of the accelerator, the DPTD based latex and dry natural rubber (NR) vulcanisates show improved mechanical properties as compared to tetramethylthiuram disulphide (TMTD) based vulcanisates. DPTD may be considered as a safe replacement for the nitrosamine generating TMTD in natural rubber based formulations.