bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain
Authors: Ohtaka‐Maruyama Chiaki , Miwa Akiko , Kawano Hitoshi , Kasai Masataka , Okado Haruo 
Published: April 2007

AbstractRP58, a novel zinc finger protein containing a POZ domain, is a sequence‐specific transcriptional repressor. To understand the role of this protein, we examined RP58 gene expression in the developing mouse brain by quantitative polymerase chain reaction (PCR) and in situ hybridization. RP58 mRNA expression was detected at embryonic day (E) 10 in the neuroepithelium, and subsequently in the ventricular zones of the cerebral cortex in the E12 embryo. Strong expression was observed in the preplate in the cerebral cortex from this stage onward. High levels of expression continued to be detected in the cortical plate and subventricular zone of the neocortex, hippocampus, and parts of the amygdala, but not in the thalamus or striatum. These results suggest that RP58 plays a crucial role in neuronal proliferation, migration, and differentiation in the developing cerebral cortex. RP58 is also expressed in the adult mouse neocortex, hippocampus, parts of the amygdala, and granule cells in the cerebellum. Double in situ hybridization using GAD67 or VGLUT1 probes revealed that RP58 is expressed in glutamatergic excitatory neurons. J. Comp. Neurol. 502:1098–1108, 2007. © 2007 Wiley‐Liss, Inc.
Mouse olfactory sensory neurons express 10,000 genes
Authors: Sammeta Neeraja , Yu Tun‐Tzu , Bose Soma C. , McClintock Timothy S. 
Published: April 2007

AbstractOlfactory epithelial cells from olfactory marker protein‐green fluorescent protein (OMP‐GFP) mice were separated by fluorescence‐activated cell sorting into a GFP+sample enriched in mature olfactory sensory neurons (OSNs) and a GFP–sample enriched in all other cells. GeneChip expression profiling of these samples provided a predictive measure of expression in OSNs. Validation tests comparing the ratio of GFP+/GFP–signal intensity against expression patterns from in situ hybridization for 189 mRNAs proved statistically significant and provided probabilities of expression in OSNs scaled according to the signal intensity ratios. These probabilities predict that, among 11,596 mRNAs detected in the GFP+sample, more than 10,000 are expressed in OSNs. Transcripts and overrepresented categories of mRNAs detected in the GFP+sample agreed with known properties of OSNs and predict additional properties. For example, ciliogenesis and spermatogenesis were overrepresented, consistent with similarities between OSN cilia and sperm flagella. Chromatin assembly mRNAs were expressed throughout the OSN cell lineage, consistent with the hypothesis that chromatin remodeling plays a role in OSN differentiation. We detected numerous signaling proteins and receptors, such as 30 nonchemosensory G‐protein‐coupled receptors, including the presynaptic glutamate receptor mGlur4 and the Wnt receptor Fzd3. The largest group of mRNAs, however, was the hundreds of transcriptional regulators that presumably determine the OSN phenotype. The absence of OMP protein in OMP‐GFP mice had no detectable effect on mRNA abundance. Within limits prescribed by the nature of microarray data and the in situ hybridization validation, these data should be useful in directing further experiments on OSN function. J. Comp. Neurol. 502:1138–1156, 2007. © 2007 Wiley‐Liss, Inc.
Type 3a and type 3b OFF cone bipolar cells provide for the alternative rod pathway in the mouse retina
Authors: Mataruga Anja , Kremmer Elisabeth , Müller Frank 
Published: April 2007

AbstractThe mammalian retina provides several pathways to relay the information from the photoreceptors to the ganglion cells. Cones feed into ON and OFF cone bipolar cells that excite ON and OFF ganglion cells, respectively. In the “classical” rod pathway, rods feed into rod bipolar cells that provide input to both the ON and the OFF pathway via AII amacrine cells. Recent evidence suggests an alternative rod pathway in which rods directly contact some types of OFF cone bipolar cells. The mouse has become an important model system for retinal research. We performed an immunohistochemical analysis on the level of light and electron microscopy to identify the bipolar cells and ganglion cells that are involved in the alternative rod pathway of the mouse retina. 1) We identify a new bipolar cell type, showing that type 3 OFF cone bipolar cells comprise two distinct cell types, that we termed 3a and 3b. Type 3a cells express the ion channel HCN4. Type 3b bipolar cells represent a hitherto unknown cell type that can be identified with antibodies against the regulatory subunit RIIβ of protein kinase A. 2) We show that both 3a and 3b cells form flat contacts at cone pedicles and rod spherules. 3) Finally, we identify an OFF ganglion cell type whose dendrites costratify with type 3a and 3b bipolar cell axon terminals. These newly identified cell types represent the basis of a neuronal circuit in the mammalian retina that could provide for an alternative fast rod pathway. J. Comp. Neurol. 502:1123–1137, 2007. © 2007 Wiley‐Liss, Inc.
Regioselective Mo‐Catalyzed Hydrostannations as Key Steps in the Synthesis of Functionalized Amino Alcohols and Heterocycles
Authors: Lin Hechun , Kazmaier Uli 
Published: April 2007

AbstractMolybdenum catalyzed hydrostannation of suitable protected propargylic amino alcohols provides the corresponding functionalized vinyl stannanes, which are useful synthetic intermediates for the combinatorial synthesis of amino alcohols and heterocycles. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Carbon fiber nanoelectrodes applied to microchip electrophoresis amperometric detection of neurotransmitter dopamine in rat pheochromocytoma (PC12) cells
Authors: Cheng Han , Huang Wei‐Hua , Chen Rong‐Sheng , Wang Zong‐Li , Cheng Jie‐Ke 
Published: April 2007

AbstractMicroelectrodes have been adopted in electrochemical detection for CE or microchip CE in recent years. In this paper, the use of nanoelectrodes (with tip diameter of 100–300 nm) as the electrochemical detector in microchip CE is firstly reported. The experimental results indicated that both the sensitivity and resolution of microchip CE with the carbon fiber nanoelectrode (CFNE) amperometric detection have been improved markedly comparing with the traditional microelectrodes. The detection limit of dopamine (S/N = 3) is 5.9×10−8 M, which is one or two orders of magnitude lower than that reported so far, and the resolution of dopamine (DA) and isoprenaline (IP) has also improved from 0.6 (using 7 μm carbon fiber microelectrodes, CFME) to 1.0. We assembled a novel and easily operated microchip CE system with end‐column amperometric detection, which allows the convenient and fast replacement of the passivated electrodes. Under the optimized condition, the RSDs of peak height and migration time are 1.47 and 0.31%, respectively (n = 40), indicating that the system displays excellent reproducibility. The nanoelectrode‐based microchip CE system has been successfully applied to the determination of DA in cultured rat pheochromocytoma (PC12) cells, and the average content of DA in an individual PC12 cell is 0.54 ± 0.07 fmol, which is in good agreement with that reported in the literature.
Simultaneous determination of Zr(IV) and Hf(IV) by CE using precolumn complexation with a [PW11O39]7− ligand
Authors: Himeno Sadayuki , Kitano Eri , Chaen Norimitsu 
Published: April 2007

AbstractA CE method was developed for the simultaneous determination of Zr(IV) and Hf(IV) at trace levels. A lacunary Keggin‐type [PW11O39]7− ligand reacted quantitatively with a mixture of trace amounts of Zr(IV) and Hf(IV) to form the so‐called ternary Keggin‐type anions [P(ZrIVW11)O40]5− and [P(HfIVW11)O40]5− in 0.010 M monochloroacetate buffer (pH 2.2). Since both ternary anions possessed different electrophoretic mobilities and high molar absorptivities in the UV region, Zr(IV) and Hf(IV) were determined simultaneously with direct UV detection at 258 nm. Each peak height was linearly dependent on the concentration of Zr(IV) or Hf(IV) in the range of 5.0×10−7–1.0×10−5 M; a detection limit of 2×10−7 M was achieved. The utility of the proposed CE method was demonstrated for the simultaneous determination of Zr(IV) and Hf(IV) in natural water samples with satisfactory results.
High MS‐compatibility of silver nitrate‐stained protein spots from 2‐DE gels using ZipPlates and AnchorChips for successful protein identification
Authors: Nebrich Grit , Herrmann Marion , Sagi Dijana , Klose Joachim , Giavalisco Patrick 
Published: April 2007

AbstractThe availability of easy‐to‐handle, sensitive, and cost‐effective protein staining protocols for 2‐DE, in conjunction with a high compatibility for subsequent MS analysis, is still a prerequisite for successful proteome research. In this article we describe a quick and easy‐to‐use methodological protocol based on sensitive, homogeneous, and MS‐compatible silver nitrate protein staining, in combination with an in‐gel digestion, employing the Millipore 96‐well ZipPlate system for peptide preparation. The improved quality and MS compatibility of the generated protein digests, as compared to the otherwise weakly MS‐compatible silver nitrate staining, were evaluated on real tissue samples by analyzing 192 Coomassie‐stained protein spots against their counterparts from a silver‐stained 2‐DE gel. Furthermore, the applicability of the experimental setup was evaluated and demonstrated by the analysis of a large‐scale MALDI‐TOF MS experiment, in which we analyzed an additional ˜1000 protein spots from 2‐DE gels from mouse liver and mouse brain tissue.
The role of phase difference components of ground motions in the torsional response of asymmetric buildings
Authors: Alexander Nicholas Andrew 
Published: April 2007

AbstractIt is demonstrated that the difference in phase content between orthogonal, horizontal, accelerograms can directly influence the effective (band‐limited) torque energy applied to a plan asymmetric structure. This is not the case where a plan asymmetric structure is excited solely by a unidirectional, horizontal, accelerogram ground motion. It is shown that this effective torque energy is well correlated with building torsional (response) acceleration energy and element ductility demands for a broad class of multistorey structures. Nonlinear time‐history analyses employing a database of accelerogram abstracted from USGS are used to quantify the influence of the phase difference content on these building responses. Bias in nonlinear time‐history analyses based on a small sample of accelerograms caused by phase difference content is discussed. Copyright © 2007 John Wiley & Sons, Ltd.
On the characteristics of ground motion rotational components using Chiba dense array data
Authors: Ghayamghamian M. R. , Nouri G. R. 
Published: April 2007

AbstractAn effort is made to examine the properties of rotational (torsional and rocking) ground motions using Chiba dense array data. The Chiba array system, located 30 km east of Tokyo, Japan, is composed of 15 boreholes with separation distances varying from 5 to 320 m. This provides a unique opportunity to examine the characteristics of rotational components. For this purpose, 17 events are considered and rotational ground motions are evaluated using spatial derivatives of translational ones. The effects of seismological parameters and separation distances between stations on properties of rotational motions are examined, showing a sudden increase in rotational motions for the earthquakes with large magnitude or PGA and decrease of these motions with increasing separation distance. While the duration of torsional motion is found to be larger than translational ones, there is no significant difference between durations of rocking and vertical motions. The effects of separation distance and earthquake magnitude on rotational response spectra are also investigated. The normalized rotational response spectra are found to be strongly affected by separation distance. The spectral ratios of rotational and translational motions are not linearly proportional to period as suggested by the previous studies. Finally, the torsional motion is predicted from translation ones for different separation distances at the site. The comparison of the predicted and the calculated torsional motions reveals a weak estimation in close separation distances (<30m) and satisfactory predictions in other cases. Copyright © 2007 John Wiley & Sons, Ltd.
Dust emission from wet and dry playas in the Mojave Desert, USA
Authors: Reynolds Richard L. , Yount James C. , Reheis Marith , Goldstein Harland , Chavez Pat , Fulton Robert , Whitney John , Fuller Christopher , Forester Richard M. 
Published: April 2007

AbstractThe interactions between playa hydrology and playa‐surface sediments are important factors that control the type and amount of dust emitted from playas as a result of wind erosion. The production of evaporite minerals during evaporative loss of near‐surface ground water results in both the creation and maintenance of several centimeters or more of loose sediment on and near the surfaces of wet playas. Observations that characterize the texture, mineralogic composition and hardness of playa – surfaces at Franklin Lake, Soda Lake and West Cronese Lake playas in the Mojave Desert (California), along with imaging of dust emission using automated digital photography, indicate that these kinds of surface sediment are highly susceptible to dust emission. The surfaces of wet playas are dynamic surface texture and sediment availability to wind erosion change rapidly, primarily in response to fluctuations in water‐table depth, rainfall and rates of evaporation. In contrast, dry playas are characterized by ground water at depth. Consequently, dry playas commonly have hard surfaces that produce little or no dust if undisturbed except for transient silt and clay deposited on surfaces by wind and water. Although not the dominant type of global dust, salt‐rich dusts from wet playas may be important with respect to radiative properties of dust plumes, atmospheric chemistry, windborne nutrients and human health. Published in 2007 by John Wiley & Sons, Ltd.
Numerical investigation of the effect of hypertensive blood pressure on cerebral aneurysm—Dependence of the effect on the aneurysm shape
Authors: Torii Ryo , Oshima Marie , Kobayashi Toshio , Takagi Kiyoshi , Tezduyar Tayfun E. 
Published: April 2007

AbstractFluid–structure interaction (FSI) computations of two cerebral aneurysms are carried out under hypertensive and normotensive blood pressures. Hypertensive blood pressure is one of the major risk factors in subarachnoid hemorrhage, which is mostly caused by the rupture of cerebral aneurysm. Since hemodynamic wall shear stress (WSS) is known to play an important role in aneurysm progression, investigating the WSS distribution in conjunction with hypertensive blood pressure is expected to provide a better understanding of aneurysms. The WSS distributions obtained from the simulations show that hypertensive blood pressure considerably affects one of the subjects but not the other. The effect is a wider spreading of the high WSS region on the aneurysm wall, which prevents the wall from weakening. It is also shown that the deformation of the aneurysm wall can alter the flow patterns in the aneurysm to diminish the stagnant flow near the apex, which is linked to the weakening of the wall. The effect of hypertensive blood pressure and wall deformation is shown to be highly dependent on individual aneurysm geometry, and that stresses the importance of subject‐specific simulations. Copyright © 2007 John Wiley & Sons, Ltd.
Selenium‐Containing Heterocycles from Isoselenocyanates: Synthesis of 5‐Amino‐2,4‐dihydro‐3H‐1,2,4‐triazole‐3‐selones
Authors: Sommen Geoffroy L. , Linden Anthony , Heimgartner Heinz 
Published: April 2007

AbstractThe reaction ofS‐methylisothiosemicarbazide hydroiodide (=S‐methyl hydrazinecarboximidothioate hydroiodide;1), prepared from thiosemicarbazide by treatment with MeI in EtOH, and aryl isoselenocyanates5in CH2Cl2affords 3H‐1,2,4‐triazole‐3‐selone derivatives7in good yield (Scheme 2, Table 1). During attempted crystallization, these products undergo an oxidative dimerization to give the corresponding bis(4H‐1,2,4‐triazol‐3‐yl) diselenides11(Scheme 3). The structure of11awas established by X‐ray crystallography.
Triterpene Esters Isolated from Leaves of Maytenus salicifoliaReissek
Authors: de Miranda Roqueline Rodrigues Silva , de Fátima Silva Grácia Divina , Duarte Lucienir Pains , Filho Sidney Augusto Vieira 
Published: April 2007

AbstractThe triterpene ester (3β)‐olean‐18‐en‐3‐yl stearate (1), together with (3β)‐urs‐12‐en‐3‐yl stearate (2), and (3β)‐lup‐20(29)‐en‐3‐yl stearate (3) were isolated from leaves of Maytenus salicifolia Reissek (Celastraceae). The structure of 1, a new compound, including its configuration, was established by 1H, 13C, and DEPT‐135 NMR data, including 2D experiments (HSQC, HMBC, COSY, and NOESY). The molecular mass (692 Da) was confirmed by gas chromatography coupled with mass spectrometry (CG/MS).
Three New Bibenzyl Derivatives from the Chinese Liverwort Marchantia polymorpha L.
Authors: Fang Lei , Guo Huai‐Fang , Lou Hong‐Xiang 
Published: April 2007

AbstractFrom the Et2O extract of the Chinese liverwort Marchantia polymorpha L., three new bibenzyl (=1,1′‐(ethane‐1,2‐diyl)bisbenzene) derivatives, compounds 1–3, were obtained, together with the two known compounds marchantin J and perrottetin E. Their structures were established by NMR and HR‐EI‐MS analyses. Polymorphatin A (1) represents a new type of bis[bibenzyl] skeleton, and compound 3 is the first example of a bibenzyl oxidatively coupled to a phenylmethanol.
Stille Cross‐Coupling of a Racemic Planar‐Chiral Ferrocene and Crystallographic Trace Analysis of Catalysis Intermediates and By‐products
Authors: Weber Immo , Heinemann Frank W. , Bakatselos Panagiotis , Zenneck Ulrich 
Published: April 2007

AbstractA pressure‐controlled procedure for the SN1 reaction of rac‐1‐[(dimethylamino)methyl]‐2‐(tributylstannyl)ferrocene (1) to rac‐1‐(phthalimidomethyl)‐2‐(tributylstannyl)ferrocene (2) was developed. Pd0‐Catalyzed Stille coupling of 2 with iodobenzene afforded rac‐1‐phenyl‐2‐(N‐phthalimidomethyl)ferrocene (5) in 74% yield; after trace enrichment by crystallization of the combined mother liquors, one single crystal of each, 5, catalysis intermediate trans‐iodo(σ‐phenyl)bis(triphenylarsino)palladium(II) (7), trans‐diiodobis(triphenylarsino)palladium(II) (8), and rac‐2,2′‐bis(phthalimidomethyl)‐1,1′‐biferrocene (9) could be isolated by crystal sorting under a microscope and characterized by X‐ray crystal structure analysis. Furthermore, 5 was deprotected to amine (11), which does even survive the Birch reduction to rac‐1‐(aminomethyl)‐2‐(cyclohexa‐2,5‐dienyl)ferrocene (12).
The influence of actual and unrequited interventions
Authors: Dominguez Kathryn M. E. , Panthaki Freyan 
Published: April 2007

AbstractIntervention operations are used by governments to manage their exchange rates but officials rarely confirm their presence in the market, leading inevitably to erroneous reports in the financial press. There are also reports of what we term, unrequited interventions, interventions that the market expects but do not materialize. In this paper we examine the effects of various types of intervention news on intra‐day exchange rate behaviour. We find that unrequited interventions have a statistically significant influence on returns, volatility and order flow, suggesting that the expectation of intervention, even when governments do not intervene, can affect currency values. Copyright © 2007 John Wiley & Sons, Ltd.
Myths and reality of foreign exchange interventions: an application to Japan
Authors: Ito Takatoshi 
Published: April 2007

AbstractThe objective of this paper is to examine what we know, and do not know, about foreign exchange interventions by the monetary authorities, and to present some new evidence on the Japanese intervention activities. With limited data, efforts are made to determine whether some conventionally made assertions are a myth or a fact. Copyright © 2007 John Wiley & Sons, Ltd.
Discounting the distant future: How much does model selection affect the certainty equivalent rate?
Authors: Groom Ben , Koundouri Phoebe , Panopoulou Ekaterini , Pantelidis Theologos 
Published: April 2007

AbstractRecent work in evaluating investments with long‐term consequences has turned towards establishing a schedule of Declining Discount Rates (DDRs). Using US data we show that the employment of models that account for changes in the interest rate generating mechanism has important implications for operationalising a theory of DDRs that depends upon uncertainty. The policy implications of DDRs are then analysed in the context of climate change for the USA, where the use of a state space model can increase valuations by 150% compared to conventional constant discounting. Copyright © 2007 John Wiley & Sons, Ltd.
Correcting for bias in retrospective data
Authors: Shachar Ron , Eckstein Zvi 
Published: April 2007

AbstractWhen panel data are not available, retrospective data are used in the estimation of dynamic choice models. However, retrospective data are not reliable. Previous studies of voting choices, for example, have shown that respondents misreport their past choices in order to appear more consistent with their current choice. Such retrospective bias leads to inconsistent estimates, especially when there is state dependence in choices. Specifically, observed persistence in retrospective data may be due to (a) true state dependence, (b) unobserved heterogeneity, and (c) retrospective bias in reporting previous choices. Whereas Heckman in his 1981 study deals with (a) and (b), we introduce a method to estimate true state dependence while accounting for both unobserved heterogeneity and retrospective reporting bias. Our method is based on modeling the reporting behavior and integrating it into the estimation. The identification strategy is based on the correlation between the reported previous choices and current exogenous variables. Using data on Israeli voters, we find that the probability that a respondent whose vote intention in 1991 differed from his or her past voting choices would lie about their past choices is 0.23. Copyright © 2007 John Wiley & Sons, Ltd.