bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene Expression Analysis of Hematopoietic Progenitor Cells Identifies Dlg7as a Potential Stem Cell Gene
Authors: Gudmundsson Kristbjorn Orri , Thorsteinsson Leifur , Sigurjonsson Olafur E. , Keller Jonathan R. , Olafsson Karl , Egeland Torstein , Gudmundsson Sveinn , Rafnar Thorunn 
Published: February 2007

AbstractInducible hematopoietic stem/progenitor cell lines represent a model for studying genes involved in self-renewal and differentiation. Here, gene expression was studied in the inducible human CD34+ acute myelogenous leukemia cell line KG1 using oligonucleotide arrays and suppression subtractive cloning. Using this approach, we identified Dlg7, the homolog of the Drosophila Dlg1 tumor suppressor gene, as downregulated at the early stages of KG1 differentiation. Similarly, Dlg7 was expressed in normal purified umbilical cord blood CD34+CD38− progenitors but not in the more committed CD34+CD38+ population. Dlg7 expression was not detected in differentiated cells obtained from hematopoietic colonies, nor was expression detected in purified T-cells, B-cells, and monocytes. When analyzed in different types of stem cells, Dlg7 expression was detected in purified human bone marrow-derived CD133+ progenitor cells, human mesenchymal stem cells, and mouse embryonic stem (ES) cells. Overexpression of Dlg7 in mouse ES cells increased their growth rate and reduced the number of EBs emerging upon differentiation. In addition, the EBs were significantly smaller, indicating an inhibition in differentiation. This inhibition was further supported by higher expression of Bmp4, Oct4, Rex1, and Nanog in EBs overexpressing Dlg7 and lower expression of Brachyury. Finally, the Dlg7 protein was detected in liver and colon carcinoma tumors but not in normal adjacent tissues, suggesting a role for the gene in carcinogenesis. In conclusion, our results suggest that Dlg7 has a role in stem cell survival, in maintaining stem cell properties, and in carcinogenesis.Disclosure of potential conflicts of interest is found at the end of this article.
Analyses of Very Early Hemopoietic Regeneration After Bone Marrow Transplantation: Comparison of Intravenous and Intrabone Marrow Routes
Authors: Li Qing , Hisha Hiroko , Yasumizu Ryoji , Fan Tian-Xue , Yang Guo-Xiang , Li Qiang , Cui Yun-Ze , Wang Xiao-Li , Song Chang-Ye , Okazaki Satoshi , Mizokami Tomomi , Cui Wen-Hao , Guo Kequan , Li Ming , Feng Wei , Katou Junko , Ikehara Susumu 
Published: February 2007

Abstract
In bone marrow transplantation (BMT), bone marrow cells (BMCs) have traditionally been injected intravenously. However, remarkable advantages of BMT via the intra-bone-marrow (IBM) route (IBM-BMT) over the intravenous route (IV-BMT) have been recently documented by several laboratories. To clarify the mechanisms underlying these advantages, we analyzed the kinetics of hemopoietic regeneration after IBM-BMT or IV-BMT in normal strains of mice. At the site of the direct injection of BMCs, significantly higher numbers of donor-derived cells in total and of c-kit+ cells were observed at 2 through 6 days after IBM-BMT. In parallel, significantly higher numbers of colony-forming units in spleen were obtained from the site of BMC injection. During this early period, higher accumulations of both hemopoietic cells and stromal cells were observed at the site of BMC injection by the IBM-BMT route. The production of chemotactic factors, which can promote the migration of a BM stromal cell line, was observed in BMCs obtained from irradiated mice as early as 4 hours after irradiation, and the production lasted for at least 4 days. In contrast, sera collected from the irradiated mice showed no chemotactic activity, indicating that donor BM stromal cells that entered systemic circulation cannot home effectively into recipient bone cavity. These results strongly suggest that the concomitant regeneration of microenvironmental and hemopoietic compartments in the marrow (direct interaction between them at the site of injection) contributes to the advantages of IBM-BMT over IV-BMT.
Disclosure of potential conflicts of interest is found at the end of this article.
Enhanced Oxide Ion Conductivity in Stabilized δ‐Bi2O3.
Authors: Punn Rita , Feteira Antonio M. , Sinclair Derek C. , Greaves Colin 
Published: February 2007

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis and cellular uptake of a MR contrast agent coupled to an antisense peptide nucleic acid – cell– penetrating peptide conjugate
Authors: Su W. , Mishra R. , Pfeuffer J. , Wiesmüller K.‐H. , Ugurbil K. , Engelmann J. 
Published: February 2007

AbstractIn order to image mRNA transcription by in vivo magnetic resonance imaging (MRI), two intracellular MR contrast agents were developed, which are composed of a Gd–DOTA complex, a peptide nucleic acid (PNA) sequence and a cell‐penetrating peptide. One was designed to bind to mRNA of dsRed (red fluorescent protein originating from Discosoma coral) by its PNA sequence, whereas the second one contains a nonsense sequence with no natural counterpart. The conjugates were synthesized using a continuous solid‐phase synthesis scheme and characterized by ESI‐MS. Fluorescence studies showed that both contrast agents could enter efficiently into 3T3 cells in a concentration‐dependent manner from 0.5 to 9.0 µM. The contrast agent was located predominantly in vesicles around the nucleus, whereas no uptake into the nucleus was observed. The results of in vitro MR studies showed a statistically significant increase of the intracellular relaxation rate R
1,cell at a labeling concentration of only 0.5 µM, thus contrast enhancement was detectable too. These results suggest that the synthesized contrast agents could label cells for optical as well as MR imaging and in future might be useful to prove specific accumulation in cells containing target mRNA. Copyright © 2007 John Wiley & Sons, Ltd.
Single‐injection ornithine decarboxylase‐directed antisense therapy using atelocollagen to suppress human cancer growth
Authors: Nakazawa Kunihiko , Nemoto Takahiro , Hata Tomoko , Seyama Yousuke , Nagahara Shunji , Sano Akihiko , Itoh Hiroshi , Nagai Yutaka , Kubota Shunichiro 
Published: February 2007

AbstractBACKGROUNDSubstantial evidence supports a direct role of ornithine decarboxylase (ODC) in the development and maintenance of human tumors. Although antisense oligonucleotide therapy targeting various genes are useful for cancer treatment, 1 of the major limitations is the problem of delivery. A novel antisense oligonucleotide delivery method is described that allows prolonged sustainment and release of ODC antisense oligonucleotides in vivo using atelocollagen.METHODSThe effect of ODC antisense oligonucleotides in the atelocollagen on cell growth of gastrointestinal cancer (MKN 45 and COLO201) and rhabdomyosarcoma (RD) was studied in vitro using a cell‐counting method with a hemocytometer. In vivo, the effect of intratumoral, intramuscular, and intraperitoneal single administration of ODC antisense oligonucleotides in the atelocollagen on tumor growth of MKN45, COLO201, and RD cells was studied. ODC activity and polyamine contents were measured.RESULTSIn vitro, ODC antisense oligonucleotides in the atelocollagen remarkably suppressed MKN45, COLO201, and RD cell growth. A single administration of antisense oligonucleotides in the atelocollagen via 3 routes remarkably suppressed the growth of MKN45, COLO201, and RD tumor over a period of 35–42 days.CONCLUSIONSAs various human cancers significantly express ODC, the results strongly suggest that this new antisense method may be of considerable value for treatment of human cancers. Cancer 2007;109:993–1002. © 2007 American Cancer Society.
Analysis of saturated free fatty acids from pollen by HPLC with fluorescence detection
Authors: You Jinmao , Zhu Fang , Zhao Wenchen , Zhao Xian'en , Suo Yourui , Liu Sujuan 
Published: February 2007

AbstractA sensitive method for the determination of free fatty acids using 2‐(2‐(anthracen‐10‐yl)‐1H‐naphtho[2,3‐d]imidazol‐1‐yl) ethyl‐p‐toluenesulfonate (ANITS) as tagging reagent with fluorescence detection has been developed. ANITS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 °C for 40 min in N,N‐dimethylformamide solvent. From the extracts of rape bee pollen samples, 20 free fatty acids were sensitively determined. Fatty acid derivatives were separated on a reversed‐phase Eclipse XDB‐C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post‐column APCI/MS in positive‐ion detection mode. ANITS‐fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision‐induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M–345]+ and m/z 345.0 (here, m/z 345 is the core structural moiety of the ANITS molecule). The fluorescence excitation and emission wavelengths of the derivatives were λex = 250 nm and λem = 512 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are >0.9999. Detection limits, at a signal‐to‐noise ratio of 3 : 1, are 24.76–98.79 fmol for the labeled fatty acids.
A Convenient Preparative Method for New 1,3‐Bis(hetaryl)‐2‐chloropropen‐1‐ones Using β‐Alkoxy‐α‐chloroalkenyllithium Compounds
Authors: Itoh Akinori , Sakai Miho , Yoshimatsu Mitsuhiro 
Published: February 2007

AbstractThe 1,3‐biaryl and 1,3‐bis(hetaryl)propen‐1‐ones 14a–e, 16, 17a–c, 19, 20a and the corresponding acetals 15a–d, 18a and 21a–d were successfully synthesized by a stepwise process: the reaction of α‐chloro‐β‐ethoxyethenyllithium compounds and aldehydes and ketones, and the successive hydration with TMSOTf. Further palladium‐catalyzed arylation of 1,3‐biaryl‐2‐chloroprop‐2‐enones proceeded with good yields.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Cystic pancreatic tumors (CPT): Predictors of malignant behavior
Authors: Javle Milind , Shah Pankaj , Yu Jihnhee , Bhagat Vishal , Litwin Alan , Iyer Renuka , Gibbs John 
Published: February 2007

AbstractBackground and ObjectivesDue to widespread use of imaging studies, increasing cystic pancreatic tumor (CPT) cases are being detected. The diagnosis of malignancy in CPT cases requires pancreatectomy. Clinical and laboratory characteristics of CPT may predict underlying malignancy.MethodsCPT cases treated between 1994 and 2004 at our institution were included. Pseudocysts were excluded. Serous cystadenoma (SCA), mucinous cystadenoma (MCA), intrapapillary mucinous tumor, cystic endocrine tumor, and lymphoepithelial cysts were classified as benign or pre‐malignant. Serous cystadenocarcinoma (SCACA), mucinous cystadenocarcinoma (MCACA), and adenocarcinoma (ACA) were classified as malignant.ResultsThirty‐five patients had histological confirmation. Median age was 65 years. Male/female ratio was higher in malignant group (P = 0.0284). Weight loss and abdominal mass were more prevalent in malignant group (P = 0.042 and 0.028, respectively). Malignant lesions were larger, associated with local invasion (superior mesenteric artery (SMA), superior mesenteric vein (SMV), portal vein (PV) complex or celiac encasement) and CA 19‐9 elevation. On univariate analyses, local invasion (P = 0.0029), negative surgical intervention (P = 0.0010), presence of ACA (P = 0.0044), or malignant CPT (P = 0.0018) were associated with shorter survival. On a multivariate analysis, local invasion was associated with shorter survival [Hazard ratio (HR) = 4.322, P = 0.0218], while surgical intervention was associated with improved survival (HR = 0.179, P = 0.0124).ConclusionMale sex, abdominal mass, weight loss, larger tumor size, local invasion, and elevated CA 19‐9 were associated with malignant CPT. J. Surg. Oncol. 2007;95:221–228. © 2007 Wiley‐Liss, Inc.
Major pancreatic resections for suspected cancer in a community‐based teaching hospital: Lessons learned
Authors: Metreveli Ramaz E. , Sahm Katherine , Abdel‐Misih Raafat , Petrelli Nicholas J. 
Published: February 2007

AbstractBackgroundThe literature reports 4–10% mortality rate, 30–60% morbidity rate, and 9–29% anastomotic leak rate after pancreaticoduodenectomy (PD) performed for periampullary tumors. These data demonstrate a linear relationship between surgical volume and outcome.MethodsThe objective of this study was to evaluate the experience of a high‐volume hospital with low‐volume pancreatoduodenectomy for suspected cancer. The study was designed as a retrospective review of medical records of all patients who underwent pancreatoduodenal resection or total pancreatectomy for a suspected periampullary carcinoma between January 1994 and December 2003. The setting of the study was a community‐based teaching hospital with a general surgery residency training program.ResultsA total of 63 patients underwent pancreatoduodenal resection or total pancreatectomy. All procedures were performed by a total of 15 different surgeons; however, 27 operations were performed by one surgeon. Pre‐operative diagnosis in most cases was either a known malignancy—27 cases (43%) or a tumor of the head of the pancreas, suspicious for malignancy—36 cases (57%). One patient underwent a total pancreatectomy. In 62 patients a pancreatoduodenal resection (Whipple procedure) was performed. Post‐operative 30‐day mortality was 4.7% (three patients). Overall in‐hospital mortality was 9.5% (six patients). Ten (16.1%) had a leak of the pancreato‐jejunal anastomosis, six of which resolved with non‐operative management. Of the remaining four patients, three died from peritonitis or consequences of erosive hemorrhage.ConclusionsPost‐operative leak of the pancreatic anastomosis represents a technical challenge. Although most of the leaks can be treated non‐operatively, those that lead to peritonitis or erosive hemorrhage warrant operative intervention. Major pancreatic resections can be performed safely with low rates of morbidity and operative mortality with careful selection of patients at a low‐volume community‐based teaching hospital. J. Surg. Oncol. 2007;95:201–206. © 2007 Wiley‐Liss, Inc.
Symmetry of electron states in semiconductor structures under a magnetic field
Authors: Tronc P. , Smirnov V. P. 
Published: February 2007

AbstractWe present a group‐theory analysis of the electron states in bulk and low‐dimensional semiconductor structures under a uniform magnetic field. The analysis takes into account the gauge transformations under the symmetry operations. It is shown that the symmetry operations commute in dots, rods and tubes whatever is the orientation of the magnetic field whereas they commute in layers only when the field is in the plane. The commutations properties allow using conventional symmetry groups. In bulk materials, the confinement of electrons within rods whose axes are parallel to the field makes it possible to derive approximate wavefunction symmetries from the rod group involving the geometrical symmetry operations common to the field and the crystal but excluding the translations that are not parallel to the field. An approximation of the same kind can also be made for low‐dimensional structures. Applications to bulk materials with the wurtzite or zinc blende structure as well as related nanostructures are presented. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Study on the nature of interaction of BrCl with HF, H2O, and NH3
Authors: Wu Junyong , Zhang Jingchang , Wang Zhaoxu , Cao Weiliang 
Published: February 2007

AbstractBy counterpoise‐corrected optimization method, the interactions of BrCl with the first‐row hydrides (HF, H2O, NH3) have been investigated at the MP2/6–311++G(3d,3p) level. To understand that the XBr‐type (X = F, O, N) structure is more stable than the corresponding hydrogen‐bonded structure in these complexes, the electronic properties were also investigated. Symmetry‐adapted perturbation theory (SAPT) analysis has been carried out to understand the nature of the weak hydrogen bond and XBr‐type interactions. On the other hand, for the weak hydrogen‐bonded complexes and the XBr‐type complexes charges transfer is well correlated with the total induction energies. © 2007 Wiley Periodicals, Inc. Int J Quantum Chem, 2007
Direct characterization of intact polypeptides by matrix‐assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry
Authors: Sampson Jason S. , Hawkridge Adam M. , Muddiman David C. 
Published: February 2007

AbstractWe report the characterization of a recently introduced hybrid ionization source, matrix‐assisted laser desorption electrospray ionization (MALDESI), coupled to a quadrupole Fourier transform ion cyclotron resonance mass spectrometry (QFT‐ICR‐MS) system. We first demonstrate the ability of MALDESI‐QFT‐ICR MS to directly analyze and provide high mass measurement accuracy (∼1 part‐per‐million) of a polypeptide using internal calibration. Second, we show the potential of MALDESI‐QFT‐ICR MS for the top‐down characterization of multiply charged polypeptide cations. Finally, we demonstrate sub‐femtomole detection limits in MALDESI‐QFT‐ICR MS using a combination of naturally occurring peptides and their respective stable isotope labeled forms. The results presented herein demonstrate the feasibility of several potential applications for MALDESI‐QFT‐ICR MS for the direct analysis of intact biological molecules. Copyright © 2007 John Wiley & Sons, Ltd.
Corticofugal influences on thalamic neurons during nociceptive transmission in awake rats
Authors: Wang Jin‐Yan , Chang Jing‐Yu , Woodward Donald J. , Baccalá Luiz A. , Han Ji‐Sheng , Luo Fei 
Published: February 2007

AbstractPain is a multidimensional phenomenon and processed in a neural network. The supraspinal, brain mechanisms are increasingly recognized in playing a major role in the representation and modulation of pain. The aim of the current study is to investigate the functional interactions between cortex and thalamus during nociceptive processing, by observing the pain‐related information flow and neuronal correlations within thalamo‐cortical pathways. Pain‐evoked, single‐neuron activity was recorded in awake Sprague‐Dawley rats with a Magnet system. Eight‐wire microarrays were implanted into four different brain regions, i.e., the primary somatosensory (SI) and anterior cingulate cortex (ACC), as well as ventral posterior (VP) and medial dorsal thalamus (MD). Noxious radiant heat was delivered to the rat hind paws on the side contralateral to the recording regions. A large number of responsive neurons were recorded in the four brain areas. Directed coherence analysis revealed that the amount of information flow was significantly increased from SI cortex to VP thalamus following noxious stimuli, suggesting that SI cortex has descending influence on thalamic neurons during pain processing. Moreover, more correlated neuronal activities indicated by crosscorrelation histograms were found between cortical and thalamic neurons, with cortical neurons firing ahead of thalamic units. On basis of the above findings, we propose that nociceptive responses are modulated by corticothalamic feedback during nociceptive transmission, which may be tight in the lateral pathway, while loose in the medial pathway. Synapse 61:335–342, 2007. © 2007 Wiley‐Liss, Inc.
Cardiovascular hemodynamics and umbilical artery N‐terminal peptide of proB‐type natriuretic peptide in human fetuses with growth restriction
Authors: Girsen A. , Ala‐Kopsala M. , Mäkikallio K. , Vuolteenaho O. , Räsänen J. 
Published: February 2007

AbstractObjectiveTo test our hypothesis that human fetal N‐terminal peptide of proB‐type natriuretic peptide (NT‐proBNP) secretion is increased in proportion to the severity of fetal cardiovascular compromise in intrauterine growth restriction.MethodsThis prospective cross‐sectional study consisted of 42 growth‐restricted fetuses who underwent Doppler ultrasonographic examination of cardiovascular hemodynamics within 7 days before delivery. Group 1 fetuses (n = 13) had normal umbilical artery (UA) velocimetry. Group 2 fetuses (n = 15) had abnormal UA and normal ductus venosus (DV) velocimetry. In Group 3 fetuses (n = 14), both UA and DV velocimetries were abnormal. At delivery, an UA blood sample was obtained for assessment of NT‐proBNP. Normal values for UA NT‐proBNP were determined in 49 neonates (control group) with uncomplicated pregnancy and delivery.ResultsGroup 3 fetuses demonstrated greater (P < 0.05) UA and descending aorta pulsatility indices (PIs) and greater DV, left hepatic vein (LHV) and inferior vena cava PIs for veins (PIVs) than fetuses in Groups 1 and 2. Weight‐indexed cardiac outputs and ventricular ejection forces were similar among the groups. Group 3 fetuses had higher (P < 0.05) UA NT‐proBNP concentration than fetuses in Groups 1 and 2. In the control group, the 95th percentile value of UA NT‐proBNP was 518 pmol/L. In Group 3, 13/14 neonates demonstrated abnormal UA NT‐proBNP levels. The corresponding incidences were 4/13 and 7/15 in Groups 1 and 2. Significant positive correlations were found between UA, DV and LHV PIVs and UA NT‐proBNP concentrations.ConclusionIn human fetal growth restriction, increased cardiac afterload and pulsatility in DV blood velocity waveform pattern are associated with elevated UA NT‐proBNP concentrations. Copyright © 2007 ISUOG. Published by John Wiley & Sons, Ltd.
Radiographic threshold values for hip screening in cerebral palsy
Authors: Hägglund Gunnar , Lauge-Pedersen Henrik , Persson Måns 
Published: February 2007

Purpose To analyse the consequences of using different radiographic measurements and different threshold values for hip screening in children with cerebral palsy (CP). Methods In a total sample of children with CP a standardised radiological follow-up of the hips was carried out as a part of a hip prevention programme. Acetabular index (AI) and migration percentage (MP) were measured on all radiographs. In this study, 1,067 radiographs of 272 children born 1992–1998 were analysed. Results Lateral displacement of the femoral head was common without acetabular dysplasia, and acetabular dysplasia occurred at a later stage than femoral head lateralisation. Hip dysplasia without lateral displacement of the femoral head was rare. In 16 of the 56 hips (29%) with AI ≥ 27° and in 23 of the 71 (32%) hips with MP ≥ 33% the values decreased below the threshold value without operative treatment. In hips with AI ≥ 30° only 2 of 31 hips (6%) and in hips with MP ≥ 40% only 5 of 44 hips (11%) decreased below the threshold values without operative treatment. Conclusions Radiographic follow-up with only measurement of the MP seems sufficient in screening for dislocation in children with CP. MP ≥ 33% is recommended as threshold for reaction or intensified observation. In children with MP ≥ 40%, the lateral displacement increased over time in most hips, thus indicating the need for operative intervention. In children with MP 33–40%, treatment should be based on other clinical signs and the progression of MP over time.
Deaths with dental anaesthetics
Authors: Bourne J. G. 
Published: February 2007

SUMMARYCase summaries are given of 16 recent deaths in the dental chair, 15 of them associated with the administration of a general anaesthetic (given in eight cases by consultant anaesthetists) and one with a local analgesia. Contrary to what is generally supposed, deaths with dental analgesia are rarely due to errors made by the anaesthetist: the usual cause is the upright or semi‐upright position of the patient. Dental patients undergoing general anaesthesia should be treated lying down.