bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of polyclonal antibodies against leek yellow stripe virus (LYSV) coat protein expressed in Escherichia coli and its application in serological diagnostics
Authors: Gupta Nitika , Rai Richa , Islam Sabina , Meena R P , Baranwal V K 
Published: April 2024

Abstract
Leek yellow stripe virus (LYSV) is one of the most important potyviruses, associated with garlic throughout the world, including India. LYSV causes stunting and yellow streaks in garlic and leek leaves and with other coinfecting viruses leading to severe symptom expression and yield reduction. In this study, we have made the first reported attempt to produce specific polyclonal antibodies to LYSV using expressed recombinant coat protein (CP), which would be useful for screening and routine indexing of the garlic germplasm. The CP gene was cloned, sequenced, and further subcloned in pET-28a(+) expression vector, which yielded ∼35 kDa fusion protein. The fusion protein was obtained in insoluble fraction after purification and its identity was confirmed by SDS-PAGE and western blotting. The purified protein was used as immunogen for production of polyclonal antisera in New Zealand white rabbit. Antisera raised, was able to recognize the corresponding recombinant proteins in western blotting, immunosorbent electron microscopy and dot immunobinding assay (DIBA). Developed antisera to LYSV (titer 1:2000) was used for screening of 21 garlic accessions in antigen coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and 16 accessions were found positive for LYSV, indicating its widespread presence within the collection tested. To the best of our knowledge, this is the first report of a polyclonal antiserum against the in-vitro expressed CP of LYSV and its successful application in diagnosis of LYSV in garlic accessions in India.
Development of the differential detection reverse transcription loop-mediated isothermal amplification method for Barley yellow mosaic virus or Japanese soil-borne wheat mosaic virus from barley
Authors: Fukuta Shiro , Kato Shuhei , Suzuki Ryoji , Arakawa Mizuho , Goto Koji , Maejima Hidekazu , Nagamine Takashi , Aoki Emiko 
Published: April 2024

Abstract
A differential detection reverse transcription loop-mediated isothermal amplification (DD-RT-LAMP) method was developed to detect either Barley yellow mosaic virus (BaYMV) or Japanese soil-borne wheat mosaic virus (JSBWMV) simultaneously. Both primer sets, which recognized either BaYMV or JSBWMV genomic RNA, amplified DNA more efficiently at 65°C using an isothermal DNA amplification and fluorescence detection device. Furthermore, these primer sets showed unique annealing curves. The peak annealing temperatures of BaYMV and JSBWMV amplification products using specific primer sets were 86.9°C–87.7°C and 84.5°C–85.0°C, respectively, and were clearly distinguishable during an annealing step following the isothermal amplification, monitored using a fluorescence detection device. In the field samples of barley (Hordeum vulgare L.) tested, BaYMV or JSBWMV were detected by DD-RT-LAMP, and the detection results of DD-RT-LAMP were correspondent with the results of reverse transcription-PCR.
Occurrence of ESBL-producing avian pathogenic Escherichia coli (APEC) isolates in spiced chicken meat in Goias, Brazil
Authors: da Silva Flávio Barbosa , Ferreira Marcos Roberto Alves , Sobrinho Iderval da Silva Júnior , Dias Márcia , Rodrigues Rafael Rodrigues , Moreira Cecília Nunes 
Published: April 2024

Abstract
Some extraintestinal pathogenic Escherichia coli isolates (ExPEC), obtained from humans and chickens avian pathogenic E. coli (APEC), share similar virulence genes. Thus, products of avian origin can be a source of human infection. Moreover, these APEC isolates are resistant to antimicrobials and can spread in the environment through the chicken feces. Although the development of multidrug-resistant (MDR) microorganisms in poultry is on the rise, healthcare entities have raised concerns since MDRs can horizontally transfer resistance genes to other microorganisms and complicate the management of human infections by MDR APEC. The results of our study showed that of 80 investigated spiced chicken meat samples, 55% were contaminated with E. coli, of which 34% (15/44) contaminate with APEC. No diarrheagenic E. coli (DEC) pathotypes were found. Twenty-six isolates were MDR E. coli. Among the APEC isolates, 87% (13/15) produced extended-spectrum beta-lactamase (ESBL). The emergence of MDR/ESBL-producing APEC with zoonotic potential for humans is extremely worrying. Therefore, further studies are required to identify the prevalence of MDR/ESBL-producing APEC in the entire chicken production chain from creation, slaughter, processing, and butchery.
Evaluation of Telemedicine Support for Medics from the Perspective of Course Instructors
Authors: Çetin Mehmet , Yıldırım Mehmet , Türkmen Volkan 
Published: April 2024

ABSTRACT

Introduction
Health services in the theater/district areas often contain difficulties. Although telemedicine has a huge potential to support medics in the area, there are challenges as well. Our aim is to evaluate the telemedicine support that can be provided to the medics from the perspective of course instructors.

Materials and Methods
Our study was carried out in The University of Health Sciences, Türkiye. All of the instructors of a medic course were asked to fill a 13-question questionnaire and Likert-type scale, sent via e-mail. Among 79 instructors, 71 of them (55 physicians, 16 nonphysicians) responded and were included in the study. The distributions of the categorical variables in the groups were analyzed with the Pearson Chi-square.

Results
Of the 71 participants, 37 (52.11%) stated that there is a need for legal and ethical regulations for telemedicine (medical liability, malpractice, obtaining consent from the casualties, civilians, cross border missions, and rank-related problems) and patients’ rights, additionally, to clarify the responsibilities of the doctors giving telemedicine support and the medics in the area. It was observed that physicians’ and nonphysician group’s opinions about the telepsychotherapy were statistically significantly different (χ2 = 8.675, P = .013).

Conclusions
Most of the instructors believed that telemedicine could carry the knowledge and skills of specialist physicians to the field of operation. Access to high-quality health services in a short time through telemedicine support can increase the courage and commitment of the personnel. It is thought that with telemedicine, medics will not feel alone in the field, their worries about making wrong decisions will decrease, and their knowledge and skills will increase. Participants were particularly concerned about who will be held responsible for problems arising from interventions carried out using telemedicine, and strict legal and medical regulations are needed.
Flickering pulsations in bright X-ray pulsars: the evidence of gravitationally lensed and eclipsed accretion column
Authors: Mushtukov Alexander A , Weng Albert , Tsygankov Sergey S , Mereminskiy Ilya A 
Published: April 2024

ABSTRACT
It is expected that the extreme mass accretion rate onto strongly magnetized neutron stars results in the appearance of accretion columns above the stellar surface. For a distant observer, rotation of a star results in periodic variations of X-ray flux. Because the mass accretion rate fluctuates around the average value, the pulse profiles are not stable and demonstrate fluctuations as well. In the case of bright X-ray pulsars, however, pulse fluctuations are not solely attributed to variations in the mass accretion rate. They are also influenced by the variable height of the columns, which is dependent on the mass accretion rate. This study delves into the process of pulse profile formation in bright X-ray pulsars, taking into account stochastic fluctuations in the mass accretion rate, the corresponding variations in accretion column geometry, and gravitational bending. Our analysis reveals that potential eclipses of accretion columns by a neutron star during their spin period should manifest specific features in pulse profile variability. Applying a novel pulse profile analysis technique, we successfully detect these features in the bright X-ray transient V 0332+53 at luminosities $\gtrsim 2\times 10^{38}\, {\rm erg\ \rm s^{-1}}$. This detection serves as compelling evidence for the eclipse of an accretion column by a neutron star. Detection of the eclipse places constraints on the relation between neutron star mass, radius, and accretion column height. Specifically, we can establish an upper limit on the accretion column height, which is crucial for refining theoretical models of extreme accretion.
Viral Receptor-Binding Protein Evolves New Function through Mutations That Cause Trimer Instability and Functional Heterogeneity
Authors: Strobel Hannah M , Labador Sweetzel D , Basu Dwaipayan , Sane Mrudula , Corbett Kevin D , Meyer Justin R 
Published: April 2024

Abstract
When proteins evolve new activity, a concomitant decrease in stability is often observed because the mutations that confer new activity can destabilize the native fold. In the conventional model of protein evolution, reduced stability is considered a purely deleterious cost of molecular innovation because unstable proteins are prone to aggregation and are sensitive to environmental stressors. However, recent work has revealed that nonnative, often unstable protein conformations play an important role in mediating evolutionary transitions, raising the question of whether instability can itself potentiate the evolution of new activity. We explored this question in a bacteriophage receptor-binding protein during host-range evolution. We studied the properties of the receptor-binding protein of bacteriophage λ before and after host-range evolution and demonstrated that the evolved protein is relatively unstable and may exist in multiple conformations with unique receptor preferences. Through a combination of structural modeling and in vitro oligomeric state analysis, we found that the instability arises from mutations that interfere with trimer formation. This study raises the intriguing possibility that protein instability might play a previously unrecognized role in mediating host-range expansions in viruses.
Discovery of a polymorphic gene fusion via bottom-up chimeric RNA prediction
Authors: Elfman Justin , Goins Lynette , Heller Tessa , Singh Sandeep , Wang Yuh-Hwa , Li Hui 
Published: April 2024

Abstract
Gene fusions and their chimeric products are commonly linked with cancer. However, recent studies have found chimeric transcripts in non-cancer tissues and cell lines. Large-scale efforts to annotate structural variations have identified gene fusions capable of generating chimeric transcripts even in normal tissues. In this study, we present a bottom-up approach targeting population-specific chimeric RNAs, identifying 58 such instances in the GTEx cohort, including notable cases such as SUZ12P1–CRLF3, TFG–ADGRG7 and TRPM4–PPFIA3, which possess distinct patterns across different ancestry groups. We provide direct evidence for an additional 29 polymorphic chimeric RNAs with associated structural variants, revealing 13 novel rare structural variants. Additionally, we utilize the All of Us dataset and a large cohort of clinical samples to characterize the association of the SUZ12P1–CRLF3-causing variant with patient phenotypes. Our study showcases SUZ12P1–CRLF3 as a representative example, illustrating the identification of elusive structural variants by focusing on those producing population-specific fusion transcripts.
FEVER: an interactive web-based resource for evolutionary transcriptomics across fishes
Authors: Montfort Jérôme , Hervas-Sotomayor Francisca , Le Cam Aurélie , Murat Florent 
Published: April 2024

Abstract
Teleost fish represent one of the largest and most diverse clades of vertebrates, which makes them great models in various research areas such as ecology and evolution. Recent sequencing endeavors provided high-quality genomes for species covering the main fish evolutionary lineages, opening up large-scale comparative genomics studies. However, transcriptomic data across fish species and organs are heterogenous and have not been integrated with newly sequenced genomes making gene expression quantification and comparative analyses particularly challenging. Thus, resources integrating genomic and transcriptomic data across fish species and organs are still lacking. Here, we present FEVER, a web-based resource allowing evolutionary transcriptomics across species and tissues. First, based on query genes FEVER reconstructs gene trees providing orthologous and paralogous relationships as well as their evolutionary dynamics across 13 species covering the major fish lineages, and 4 model species as evolutionary outgroups. Second, it provides unbiased gene expression across 11 tissues using up-to-date fish genomes. Finally, genomic and transcriptomic data are combined together allowing the exploration of gene expression evolution following speciation and duplication events. FEVER is freely accessible at https://fever.sk8.inrae.fr/.
Nα-acetyltransferase NAA50 mediates plant immunity independent of the Nα-acetyltransferase A complex
Authors: Armbruster Laura , Pożoga Marlena , Wu Zhongshou , Eirich Jürgen , Thulasi Devendrakumar Karen , De La Torre Carolina , Miklánková Pavlina , Huber Monika , Bradic Fabian , Poschet Gernot , Weidenhausen Jonas , Merker Sabine , Ruppert Thomas , Sticht Carsten , Sinning Irmgard , Finkemeier Iris , Li Xin , Hell Rüdiger , Wirtz Markus 
Published: April 2024

Abstract
In humans and plants, 40% of the proteome is cotranslationally acetylated at the N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex is comprised of the catalytic subunit Nα-acetyltransferase 10 (NAA10) and the ribosome-anchoring subunit NAA15. The regulatory subunit Huntingtin Yeast Partner K (HYPK) and the acetyltransferase NAA50 join this complex in humans. Even though both are conserved in Arabidopsis (Arabidopsis thaliana), only AtHYPK is known to interact with AtNatA. Here we uncover the AtNAA50 interactome and provide evidence for the association of AtNAA50 with NatA at ribosomes. In agreement with the latter, a split-luciferase approach demonstrated close proximity of AtNAA50 and AtNatA in planta. Despite their interaction, AtNatA/HYPK and AtNAA50 exerted different functions in vivo. Unlike NatA/HYPK, AtNAA50 did not modulate drought tolerance or promote protein stability. Instead, transcriptome and proteome analyses of a novel AtNAA50-depleted mutant (amiNAA50) implied that AtNAA50 negatively regulates plant immunity. Indeed, amiNAA50 plants exhibited enhanced resistance to oomycetes and bacterial pathogens. In contrast to what was observed in NatA-depleted mutants, this resistance was independent of an accumulation of salicylic acid prior to pathogen exposure. Our study dissects the in vivo function of the NatA interactors HYPK and NAA50 and uncovers NatA-independent roles for NAA50 in plants.
Should obstetric mortality be an inalienable right?
Authors: Badejoko Olusegun Olalekan , Awowole Ibraheem Olayemi , Ubom Akaninyene Eseme , Olayemi Olaniyi Joseph 
Published: April 2024

Abstract

Background
Obstetricians often times find themselves in a conflict of right and duty with their patients, when these patients refuse recommended treatment. On the one hand, the obstetrician, aiming to fulfil the duty of care, recommends a treatment in the best interest of the woman. The woman, on the other hand, exercising her right of self-determination and autonomy, declines the recommended treatment.

Materials and Methods
A search was conducted for literature, articles and case reports on the subject on PubMed/MEDLINE and Google Scholar using the keywords: medical ethics, medical law, obstetric mortality, maternal medicine, foetal medicine, patient autonomy, informed consent, right to life and right to liberty.

Results
Opinions have historically differed on whether maternal or foetal rights should be deferred to in situations where pregnant women refuse obstetric interventions. So also have legal decisions on the issue. The general consensus is, however, to respect a woman's refusal of recommended medical treatment, in deference to her right of self-determination and autonomy. The obstetric outcomes in such instances are however, often times, unfavourable.

Conclusion
The ethics of patient care in the face of conflicting rights deserves renewed examination and discourse.
Exact Solution for Rotating Black Holes in Parity-Violating Gravity
Authors: Tahara Hiroaki W H , Takahashi Kazufumi , Minamitsuji Masato , Motohashi Hayato 
Published: April 2024

Abstract
It has recently been pointed out that one can construct invertible conformal transformations with a parity-violating conformal factor, which can be employed to generate a novel class of parity-violating ghost-free metric theories from general relativity. We obtain exact solutions for rotating black holes in such theories by performing the conformal transformation on the Kerr solution in general relativity, which we dub conformal Kerr solutions. We explore the geodesic motion of a test particle in the conformal Kerr spacetime. While null geodesics remain the same as those in the Kerr spacetime, timelike geodesics exhibit interesting differences due to an effective external force caused by the parity-violating conformal factor.
Pauli–Villars Regularization of Kaluza–Klein Casimir Energy with Lorentz Symmetry
Authors: Matsui Hiroki , Sakamura Yutaka 
Published: April 2024

Abstract
The Pauli–Villars regularization is appropriate to discuss the UV sensitivity of low-energy observables because it mimics how the contributions of new particles at high energies cancel large quantum corrections from the light particles in the effective field theory. We discuss the UV sensitivity of the Casimir energy density and pressure in an extra-dimensional model in this regularization scheme, and clarify the condition on the regulator fields to preserve the Lorentz symmetry of the vacuum state. Some of the conditions are automatically satisfied in spontaneously broken supersymmetric models, but supersymmetry is not enough to ensure the Lorentz symmetry. We show that the necessary regulators can be introduced as bulk fields. We also evaluate the Casimir energy density with such regulators, and its deviation from the result obtained in the analytic regularization.
Long-term night shift work, genetic predisposition and risk of incident asthma: a prospective cohort study
Authors: Hu B , Xie Y , Yin H , Yang S , You X , Ma J , Yang L 
Published: April 2024

Summary

Background
Evidence about a potential link between current and lifetime night shift work and risk of incident asthma is insufficient.

Aim
To investigate the association of current and lifetime night shift work with risk of incident asthma, and the modified effect of genetic susceptibility on this association.

Design and methods
We included 253 773 individuals with complete night shift work information in the UK biobank. We calculated the standard polygenetic risk score (PRS) for asthma. The Cox proportional hazard models were conducted to estimate hazard ratios and 95% CIs.

Results
After multivariable adjustments, we found that current night shift work was associated with an increased risk of incident asthma in a dose–response fashion (P for trend &lt; 0.001). Compared with day workers, those working usual/permanent night shifts had a 17% (95% CI: 1.04–1.33) higher risk of asthma incidence. In addition, we observed significant dose-dependent relationships of longer lifetime duration or frequency of night shift work with elevated risk of asthma incidence (all P for trend &lt; 0.05). Compared with never night shift workers, those with a duration (≥5 years) or frequency (≥8 nights/month) of night shift work exhibited a 20% (95% CI: 1.03–1.39) or 22% (95% CI: 1.03–1.44) higher risk of incident asthma, respectively. Moreover, the elevated risk of incident asthma related to current and lifetime night shift work exposure was strengthened by high PRS, although no significant shift work–PRS interactions were detected.

Conclusion
Both current and lifetime night shift work may increase the risk of incident asthma, regardless of genetic predisposition to asthma.
Sustained delivery of NT-3 and curcumin augments microenvironment modulation effects of decellularized spinal cord matrix hydrogel for spinal cord injury repair
Authors: Chen Jiaxin , Cheng Xing , Yu Zhengran , Deng Rongli , Cui Rui , Zhou Jing , Long Houqing , Hu Yong , Quan Daping , Bai Ying 
Published: April 2024

Abstract
Decellularized extracellular matrix hydrogel, especially that derived from spinal cord (DSCM hydrogel), has been actively considered as a functional biomaterial for remodeling the extracellular matrix of the native tissue, due to its unique characteristics in constructing pro-regenerative microenvironment for neural stem cells (NSCs). Furthermore, DSCM hydrogel can provide multiple binding domains to growth factors and drugs. Therefore, both exogenous neurotrophic factors and anti-inflammatory drugs are highly desired to be incorporated into DSCM hydrogel, which may synergistically modulate the complex microenvironment at the lesion site after spinal cord injury (SCI). Herein, neurotrophin-3 (NT-3) and curcumin (Cur) were integrated into DSCM hydrogel for SCI therapy. Due to different affinities to the DSCM hydrogel, NT-3 underwent a controlled release manner, while curcumin released explosively within the first 24 h, followed by rather sustained but slower release. The integration of both NT-3 and curcumin significantly enhanced NSCs proliferation and their neuronal differentiation. Meanwhile, the release of curcumin promoted macrophages polarization into anti-inflammatory subtypes, which further facilitated NSCs differentiation into neurons. The in situ injected DSCM + NT3 + Cur hydrogel exerted superior capability in alleviating the inflammatory responses in rat contused spinal cord. Compared to DSCM hydrogel alone, DSCM + NT3 + Cur hydrogel more significantly promoted the recruitment of NSCs and their neuronal differentiation at the lesion site. These outcomes favored functional recovery, as evidenced by the improved hind limb movement. Overall, the bioactive DSCM hydrogel can serve as a multifunctional carrier for cooperatively release of growth factors and drugs, which significantly benefits microenvironment regulation and nerve regeneration after SCI.
Policy Targeting under Network Interference
Authors: Viviano Davide 
Published: April 2024

Abstract
This article studies the problem of optimally allocating treatments in the presence of spillover effects, using information from a (quasi-)experiment. I introduce a method that maximizes the sample analogue of average social welfare when spillovers occur. I construct semi-parametric welfare estimators with known and unknown propensity scores and cast the optimization problem into a mixed-integer linear program, which can be solved using off-the-shelf algorithms. I derive a strong set of guarantees on regret, i.e. the difference between the maximum attainable welfare and the welfare evaluated at the estimated policy. The proposed method presents attractive features for applications: (i) it does not require network information of the target population; (ii) it exploits heterogeneity in treatment effects for targeting individuals; (iii) it does not rely on the correct specification of a particular structural model; and (iv) it accommodates constraints on the policy function. An application for targeting information on social networks illustrates the advantages of the method.