bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microfluidic Chip‐Assisted Upconversion Luminescence Biosensing Platform for Point‐of‐Care Virus Diagnostics
Authors: Liu Yuan , Lao Xinyue , Wong Man‐Chung , Song Menglin , Lai Huang , Wang Pui , Ma Yingjin , Li Lihua , Yang Mo , Chen Honglin , Hao Jianhua 
Published: March 2024

AbstractEpidemics caused by multiple viruses continue to emerge, which have brought a terrible impact on human society. Identification of viral infections with high sensitivity and portability is of significant importance for the screening and management of diseases caused by viruses. Herein, a microfluidic chip (MFC)‐assisted upconversion luminescence biosensing platform is designed and fabricated for point‐of‐care virus detection. Upconversion nanoparticles with excellent stability are successfully synthesized as luminescent agents for optical signal generation in the portable virus diagnostic platform. The relevant investigation results illustrate that the MFC‐assisted virus diagnostic platform possesses outstanding performance such as good integration, high sensitivity (1.12 pg mL−1), ease of use, and portability. In addition, clinical sample test result verifies its more prominent virus diagnostic properties than commercially available rapid test strips. All of these thrilling capabilities imply that the designed portable virus diagnostic platform has great potential for future virus detection applications.
Van der Waals Colloidal Crystals
Authors: Cho YongDeok , Park Sung Hun , Kwon Min , Kim Hyeon Ho , Huh Ji‐Hyeok , Lee Seungwoo 
Published: March 2024

AbstractA general guiding principle for colloidal crystallization is to tame the attractive enthalpy such that it slightly overwhelms the repulsive interaction. As‐synthesized colloids are generally designed to retain a strong repulsive potential for the high stability of suspensions, encoding appropriate attractive potentials into colloids has been key to their crystallization. Despite the myriad of interparticle attractions for colloidal crystallization, the van der Waals (vdW) force remains unexplored. Here, it is shown that the implementation of gold cores into silica colloids and the resulting vdW force can reconfigure the pair potential well depth to the optimal range between −1 and −4 kBT at tens of nanometer‐scale colloidal distances. As such, colloidal crystals with a distinct liquid gap can be formed, which is evidenced by photonic bandgap‐based diffractive colorization.
CO Cofeeding Affects Product Distribution in CH3Cl Coupling over ZSM‐5 Zeolite: Pressure Twists the Plot
Authors: Zhang Zihao , Vanni Matteo , Wu Xiangkun , Hemberger Patrick , Bodi Andras , Mitchell Sharon , Pérez‐Ramírez Javier 
Published: March 2024

AbstractC1 coupling reactions over zeolite catalysts are central to sustainable chemical production strategies. However, questions persist regarding the involvement of CO in ketene formation, and the impact of this elusive oxygenate intermediate on reactivity patterns. Using operando photoelectron photoion coincidence spectroscopy (PEPICO), we investigate the role of CO in methyl chloride conversion to hydrocarbons (MCTH), a prospective process for methane valorization with a reaction network akin to methanol to hydrocarbons (MTH) but without oxygenate intermediates. Our findings reveal the transformative role of CO in MCTH at the low pressures, inducing ketene formation in MCTH and boosting olefin production, confirming the Koch carbonylation step in the initial stages of C1 coupling. We uncover pressure‐dependent product distributions driven by CO‐induced ketene formation, and its subsequent desorption from the zeolite surface, which is enhanced at low pressure. Inspired by the above results, extension of the co‐feeding approach to CH3OH as another simple oxygenate showcases the additional potential for improved catalyst stability in MCTH at ambient pressure.
Predicting Lewis Acidity: Machine Learning the Fluoride Ion Affinity of p‐Block‐Atom‐Based Molecules
Authors: Sigmund Lukas M. , S. Shree Sowndarya , Albers Andreas , Erdmann Philipp , Paton Robert S. , Greb Lutz 
Published: March 2024

Abstract“How strong is this Lewis acid?” is a question researchers often approach by calculating its fluoride ion affinity (FIA) with quantum chemistry. Here, we present FIA49k, an extensive FIA dataset with 48,986 data points calculated at the RI‐DSD‐BLYP‐D3(BJ)/def2‐QZVPP//PBEh‐3c level of theory, including 13 different p‐block atoms as the fluoride accepting site. The FIA49k dataset was used to train FIA‐GNN, two message‐passing graph neural networks, which predict gas and solution phase FIA values of molecules excluded from training with a mean absolute error of 14 kJ mol−1 (r2=0.93) from the SMILES string of the Lewis acid as the only input. The level of accuracy is notable, given the wide energetic range of 750 kJ mol−1 spanned by FIA49k. The model's value was demonstrated with four case studies, including predictions for molecules extracted from the Cambridge Structural Database and by reproducing results from catalysis research available in the literature. Weaknesses of the model are evaluated and interpreted chemically. FIA‐GNN and the FIA49k dataset can be reached via a free web app (www.grebgroup.de/fia‐gnn).
A waterborne polyurethane‐based noise‐reducing material and factors influencing its damping and noise‐reducing performance
Authors: Wu Xingchi , Cao Xuejuan , Miao Chengcheng , Yuan Ying , Yang Xiaoyu , Shu Li 
Published: March 2024

AbstractIn this study, damping coatings were used to dissipate low‐ and medium‐frequency (200–1600 Hz) traffic noise. A waterborne polyurethane/polyacrylate (WPUA) emulsion with superior damping and mechanical properties was prepared by blending polyurethane with commercial waterborne polyacrylate (SETAQUA 6754). The emulsion was combined with sound‐absorbing polyester fibers (PFs) to develop a PF/WPUA damping coating. SETAQUA 6754 improved the loss factor and mechanical properties of the waterborne resin. The composition ratio, molecular chain length, and crosslinking degree improved the damping properties of WPUA by modulating its glass transition temperature, molecular chain friction, hydrogen bonding, and molecular chain free volume. When the thickness of the waterborne damping coating was 1 cm, PF/WPUA reduced the noise by 7 dB at 200 Hz and 23 dB at 1600 Hz, indicating the effectiveness of the PF/WPUA coating in reducing reduce low‐ and medium‐frequency traffic noise.
Recent Advances in NHC‐Catalyzed Chemoselective Activation of Carbonyl Compounds
Authors: Huang Yixian , Peng Xiaolin , Li Tingting 
Published: March 2024

AbstractN‐Heterocyclic carbenes (NHCs) catalysts have been employed as effective tools in the development of various reactions, which have made notable contributions in developing diverse reaction modes and generating significant functionalized molecules. This review provides an overview of the recent advancements in the chemo‐ and regioselective activation of different aldehydes using NHCs, categorized into five parts based on the different activation modes. A brief conclusion and outlook is provided to stimulate the development of novel activation modes for accessing functional molecules.
Berechnung von Holz‐Beton‐Verbunddecken mit diskreten Schubverbindungsmitteln nach dem Kraftgrößenverfahren
Authors: Zhu Jianzhong 
Published: March 2024

AbstractIn diesem Beitrag wird gezeigt, dass Holz‐Beton‐Verbunddecken mit diskontinuierlich angeordneten Verbindungsmitteln nach dem baustatischen Kraftgrößenverfahren mit wenig Rechenaufwand berechnet werden können. Die Rechenergebnisse stimmen exakt überein, wenn nach dem Stabwerksmodell die Pendelstäbe immer dicht angeordnet werden und ihre Dehnsteifigkeit immer größer wird. Zur Berechnung der Einheitszustände wird der Begriff Quermoment eingeführt und die analytischen Lösungen werden hergeleitet. Durch Überlagerungen gewinnt man einen guten Einblick in das komplizierte Tragverhalten von Holz‐Beton‐Verbunddecken.
Emotions and motivations of gambling: A comparison between scratch card, slot‐machines, and casino gamblers
Authors: Ferro Laura , Monaci Maria Grazia , Scacchi Luca 
Published: March 2024

AbstractIntroductionIn the last 20 years, gambling has become more and more widespread in Italy. The aim of the present study is to compare the motivations for gambling and the emotions felt while gambling in three different subgroups: scratch card gamblers, slot‐machine gamblers, and casino gamblers.MethodsThree versions of a questionnaire have been distributed in a casino, in scratch card vendors, and slot‐machines venues. All versions included sociodemographic variables, the two‐question Lie‐Bet instrument, a scale for motivations toward gambling, and a list of positive and negative emotions felt while gambling.ResultsParticipants (N = 425, F 47.5%) are gamblers potentially pathological (N = 162, 38.1%) and not (N = 263, 61.9%). Different games seem connected to different motivations and emotions: the scratch gamblers show less Coping and Social Motivation and experience less Negative Emotion. However, the motivation and emotion most intensely experienced by the gamblers (Enhancement and the Positive Emotions) do not show differences between the different types of games.ConclusionThe pathological gamblers have a more intense and internal connection with the game (have more Enhancement and Coping motives, Positive and Negative Emotion). In sum, our findings support the theoretical hypothesis that gambling can be a tool for regulating emotions.
Time‐dependent systolic blood pressure within 72 h after endovascular treatment in large vessel occlusion stroke
Authors: Jiang Xin , Fang Jinghuan , Gao Lijie , Dong Shuju , Wang Jian , Hu Fayun , He Li 
Published: March 2024

AbstractBackgroundThe association of systolic blood pressure (SBP) and ischemic stroke outcome has recently been proved to be varied at different time points within 72 h after acute ischemic stroke onset; however, the specific status of how SBP affects prognosis at different time points within 72 h after endovascular treatment (EVT) among patients with large vessel occlusion (LVO) remains unclear.MethodsConsecutive LVO patients treated with EVT were enrolled in our study. BP data were collected at eight time points (1, 2, 4, 8, 16, 24, 48, and 72 h post‐EVT). Outcome measure of interest was functional dependence, which was defined as mRS >2 at 90 days.ResultsA total of 406 LVO patients treated with EVT from 2016 to 2022 were included. At 16 h after EVT, the relationship between SBP and functional dependence showed a nonlinear association. At other time points after EVT, SBP had linear relationships with functional dependence. Furthermore, higher SBP, as either a linear or quadratic term, had an adverse effect on functional outcome. In addition, three SBP trajectories were observed, and the high‐to‐low group was independently associated with functional dependence.ConclusionTaken together, higher SBP within the first 72 h after EVT has a time‐dependent association with adverse clinical outcomes. Optimal blood pressure management during the first 72 h after EVT may be important to improve clinical outcome.
Thrombolysis increases the risk of persistent headache attributed to ischemic stroke: A prospective observational study
Authors: Zhang Yi , Qu Wensheng , Ayata Cenk , Kong Qianqian , Zhao Jing , Zhou Xirui , He Dan , Yu Zhiyuan , Huang Hao , Luo Xiang 
Published: March 2024

AbstractBackground and objectivePersistent headache attributed to ischemic stroke (PHPIS) is increasingly acknowledged and was added to the 2018 ICHD‐3. Intravenous thrombolysis (IVT) is a common treatment for acute ischemic stroke. It remains unknown whether this treatment influences the occurrence of a persistent poststroke headache. We aimed to describe the incidence and clinical characteristics of persistent headaches occurring after acute ischemic stroke in patients with or without IVT and explore the risk factors.MethodsA prospective observational study was performed between the 234 individuals who received IVT and 226 individuals without IVT in 5 stroke units from Wuhan, China. Subjects were followed for 6 months after stroke via a structured questionnaire.ResultsAge, gender, vascular risk factors, and infarct location/ circulation distribution did not differ between the groups, although IVT group had higher initial NIHSS scores. At the end of the follow‐up, 12.0% (55/460) of subjects reported persistent headaches after ischemic stroke. The prevalence of persistent headache was significantly higher in the IVT group than non‐IVT group (15.4% vs. 8.4%, p = .021). Patients with younger age (p = .033; OR 0.97; 95% CI 0.939–0.997), female sex (p = .007; OR 2.40; 95% CI 1.269–4.520), posterior circulation infarct (p = .024; OR 2.19; 95% CI 1.110–4.311), and IVT (p = .005; OR 2.51; 95% CI 1.313–4.782) were more likely to develop persistent headache after ischemic stroke.ConclusionThe potential influence of IVT should be considered when assessing persistent poststroke headache. Future studies will investigate the underlying mechanisms.
Sleep disorders and criminal behavior
Authors: Watson Clarence , Weiss Kenneth J. 
Published: March 2024

AbstractBehaviors that would otherwise be considered criminal acts, but occur in the context of a sleep disorder, pose challenges to the traditional application of legal principles of criminal responsibility. Determining the degree to which consciousness is present during such behaviors becomes a necessary step in assigning criminal culpability. Historically, legal defense theories of unconsciousness, automatism, and insanity have been raised to negate culpability for parasomnia related behaviors. Accordingly, proper assessment of sleep disorders in the context of criminal charges becomes critical in assisting the functions of the justice system. This article reviews principles related to the legal tradition, expert assessment, and elements of expert testimony related to criminal behaviors and sleep disorders.
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions
Authors: Coplan Louis , Zhang Zhe , Ragone Nicole , Reeves John , Rodriguez Audrey , Shevade Aishwarya , Bak Hanne , Tustian Andrew D. 
Published: March 2024

AbstractRecombinant adeno‐associated viral vectors (rAAVs) are one of the most used vehicles for gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve product yield, we optimized the suspension production process of rAAV8 vectors carrying a proprietary transgene using a commercially available transfection reagent, FectoVIR‐AAV. Using a miniaturized automated 250 mL scale bioreactor system, we generated models of vector genome (vg) titer, capsid (cp) titer, and Vg:Cp percentage from two multivariate design of experiment studies, one centered around bioreactor operating parameters, and another based on the transfection conditions. Using the optimized process returned from these models, the vector genome titer from the bioreactor was improved to beyond 1 × 1012 vg/mL. Five critical parameters were identified that had large effects on the pre‐purification vector quantity—the transfection pH, production pH, complexation time, viable cell density at transfection, and transfection reagent to DNA ratio. The optimized process was further assessed for its performance extending to six AAV serotypes, namely AAV1, AAV2, AAV5, AAV6, AAV8, and AAV9 carrying a transgene encoding for green fluorescent protein (GFP). Five of the six serotypes returned higher vector genome titers than the control condition. These data suggest that the choice of transfection reagent is a major factor in improving vector yield. The multivariate design of experiment approach is a powerful way to optimize production processes, and the optimized process from one AAV vector can to some extent be generalized to other serotypes and transgenes to accelerate development timelines of new programs.
Human anatomy curriculum reform for undergraduate nursing students: An exploratory study
Authors: Yao Qianyin , Cheng Yatao , Wang Wen , Yu Xintian 
Published: March 2024

AbstractThis study aims to cultivate students' independent learning capacity, promote the interdisciplinary integration of “nursing + anatomy,” and establish a curriculum system to enhance applied nursing abilities based on project‐based teaching reform of everyday clinical nursing operations. A total of 151 second‐year (class of 2021) undergraduate nursing students at a Chinese university were selected for this study. By adjusting the curriculum, reconstructing the teaching contents, employing the “hybrid + flip” teaching method based on BOPPPS (bridge‐in, outcomes, preassessment, participatory learning, post‐evaluation, summary), and implementing a teaching system based on the “three re‐three linkage,” a Human Anatomy curriculum with a focus on basic anatomical knowledge was developed and connected with nursing clinical operation practice. The restructuring of the course content received unanimous recognition from both the teaching staff and the students. Notably, students in the class of 2021 achieved significantly higher grades than did students in the class of 2020, who received traditional face‐to‐face instruction (p < 0.01). These results indicate enhanced clinical application skills among the former group of students. following the implementation of instructional reforms during one semester, students exhibited notable improvements in motivation, program implementation, self‐management, and interpersonal communication. A statistically significant increase in overall scores for self‐directed learning capacities over the preinstructional period was observed (p < 0.05). Furthermore, the findings of the student satisfaction surveys reflected highly favorable perceptions of the enriched instructional format, high levels of course engagement, frequent faculty–student interactions, and augmented overall competence. The practical implementation of the reform in the context of a Human Anatomy course for undergraduate nursing students led to significant positive outcomes, thereby enhancing the effectiveness of teaching and learning. Students' clinical application abilities and self‐directed learning capacities notably improved, while overall satisfaction with the course remained high.
Successful treatment of refractory prurigo nodularis with abrocitinib
Authors: Sun Fang , Wu Zhenzhen 
Published: March 2024

Key Clinical MessagePrurigo nodularis is frequently difficult to manage with conventional therapy. Given the pathogenesis and refractory nature, we demonstrate a case in which inhibition of JAK–STAT signaling may significantly improve prurigo nodularis. Based on the results, we would like to draw a conclusion that abrocitinib as an inhibitor of Jak is a promising choice for the treatment of prurigo nodularis.
Single‐Carbon Atom Doping Reactions Using Atomic Carbon and Its Equivalents
Authors: Fujimoto Hayato , Tobisu Mamoru 
Published: March 2024

AbstractAtomic carbon, a zerovalent atom with four non‐bonded electrons, has potential utility in organic synthesis as platforms for the formation of four new covalent bonds at the carbon center. Single‐carbon atom doping (SCAD) is the process of inserting an atomic carbon into a reactant molecule with no loss of atoms in the reactant. In this Concept, we overview SCAD reactions, which can be classified by physical or chemical methods.
Enantiomeric purity of synthetic therapeutic peptides: A review
Authors: Badgujar Devendra , Paritala Sree Teja , Matre Shubham , Sharma Nitish 
Published: March 2024

AbstractSynthetic therapeutic peptides are a complex and popular class of pharmaceuticals. In recent years, peptides with proven therapeutic activity have gained significant interest in the market. The determination of synthetic peptide enantiomeric purity plays a critical role in the evaluation of the quality of the medicine. Since racemization is one of the most common side reactions occurring in AAs or peptides, enantiomeric impurities such as D‐isomers can form during the peptide synthesis or can be introduced from the starting materials (e.g., AAs). The therapeutic effect of a synthetic or semi‐synthetic bioactive peptide molecule depends on its AA enantiomeric purity and secondary/tertiary structure. Therefore, the enantiomeric purity determination for synthetic peptides is supportive for interpreting unwanted therapeutic effects and determining the quality of synthetic peptide therapeutics. However, enantiomeric purity analysis encounters formidable analytical challenges during chromatographic separation, as D/L isomers have identical physical–chemical properties except stereochemical configuration. To ensure peptides AA stereochemical configuration whether in the free or bound state, sensitive and reproducible quantitative analytical method is mandatory. In this regard, numerous analytical techniques were emerged for the quantification of D‐isomeric impurities in synthetic peptides, but still, very few reports are available in the literature. Thus, the purpose of this paper is to provide an overview of the importance, regulatory requirements, and various analytical methods used for peptide enantiomeric purity determination. In addition, we discussed the available literature in terms of enantiomeric impurity detection, common hydrolysis procedural aspects, and different analytical strategies used for sample preparation.
Feasible Synthesis of N,O‐Bidentate Difluoroboron Chromophores through Direct Functionalization of Quinoxalin‐2(1H)‐ones with Ketones in One Shot
Authors: Zhang Shuaichen , Zhang Jing , Ling Xiang , Zhao Yifan , Ma Yue , Sun Peng 
Published: March 2024

Comprehensive SummaryOrganic difluoroboron complexes are a kind of potential platforms for a wide range of applications owing to their excellent photophysical properties. Herein, we have explored a simple and direct synthesis methodology for a library of N,O‐bidentate difluoroboron complexes from quinoxalin‐2(1H)‐ones and ketones in one shot. The photophysical properties of the generated complexes were evaluated and the application potential of these compounds on subcellular imaging was also explored.
Artemordins A—S, Cadinane‐Type Sesquiterpenoid Dimers from Artemisia ordosica and Their Antihepatoma Activities
Authors: Wang Yuan , Li Tian‐Ze , Ma Yun‐Bao , Geng Chang‐An , Wang Yong‐Cui , Chen Ji‐Jun 
Published: March 2024

Comprehensive SummaryNineteen new cadinane‐involving sesquiterpenoid dimers, artemordins A—S (1—19), together with 13 known SDs (20—32) were isolated from Artemisia ordosica. Their structures and absolute configurations were established by comprehensive spectral analyses, X‐ray single crystal diffraction, theoretical ECD, and NMR calculations. Chemically, artemordins A—F (1—6) were the first examples of two cadinane units constructed by unprecedented C‐3−C‐15′ or C‐3−C‐13′ single bond with an oxido‐rearranged 6/5/6/6 fused ring system; artemordins G—K (7—11) were biogenetically connected by [4 + 2] cycloaddition reaction and artemordins G—J (7—10) possessed a novel 5/6/6/6/6/6/5‐heptacyclic fused ring system. Artemordins L—S (12—19) were formed by esterification, which involved three different types of sesquiterpenoids. Antihepatoma assay suggested that the most active compounds, artemordins B and H (2 and 8), exhibited inhibitory activities on three hepatoma cell lines with IC50 values of 26.9 and 25.1 μmol/L (HepG2), 29.5 and 18.3 μmol/L (Huh7), 19.7 and 15.7 μmol/L (SK‐Hep‐1).