bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimation of pop-up satellite archival tag initial surface position: applications for eastern Bering Sea crab research
Authors: Nault Andrew J. , Gaeuman William B. , Daly Benjamin J. , Vanek Vicki A. 
Published: April 2024

Abstract
Background
Climate change is reshaping Bering Sea crab distributions and recent population declines have elevated the urgency in understanding spatial dynamics in relation to management boundaries. While pop-up satellite archival tags (PSATs) can provide fishery-independent movement information, a high level of spatial resolution is needed to evaluate small-scale (i.e., 10 s of km) movements of crabs. Because PSATs drift at the surface prior to acquisition of a satellite-estimated location (via Argos), the accuracy of pop-up location (i.e., animal terminal position) estimates depends on the ability to accurately estimate drift error. We deployed PSATs (n = 36) on fixed-position moorings in Bristol Bay and Marmot Bay, Alaska to validate a new method to estimate tag pop-up location and an associated error ellipse that uses in situ drift data from surfaced tags to estimate drift error. Estimated pop-up location was compared to the location of tag surfacing (i.e., the tag’s fixed position) and to an alternative estimate (i.e., an early satellite-estimated location). Additional tags were deployed on mature male red king crab (Paralithodes camtschaticus) in Bristol Bay during 2020 (n = 84) and 2021 (n = 90) to compare estimates of tag drift error and crab displacement derived using the method.

Results
For red king crab tags with pop-up location estimates in 2020 (n = 79) and 2021 (n = 46), mean drift error was 9% and 44% of mean crab displacement, respectively. For fixed-position PSATs with pop-up location estimates (n = 27), mean distance between the estimated pop-up location and the tag’s fixed position was 2.0 km, representing a mean improvement in accuracy of 51% over alternative estimates. Corresponding error ellipse estimates either encompassed the tag’s fixed position (n = 11) or their boundaries were a mean distance of 1.3 km (n = 16) from the fixed position.

Conclusions
Our method improves pop-up location estimates for PSAT-tagged animals and is particularly well suited for crabs and other slow-moving benthic marine species. The improved estimates enhance PSAT utility for addressing management and ecological questions that require a fine spatial resolution, such as movement near management boundaries or defining essential habitat for important life history events.
NF1-Driven Rhabdomyosarcoma Phenotypes: A Comparative Clinical and Molecular Study of NF1-Mutant Rhabdomyosarcoma and NF1-Associated Malignant Triton Tumor
Authors: de Traux de Wardin Henry , Dermawan Josephine K. , Vanoli Fabio , Jiang Samuel C. , Singer Samuel , Chi Ping , Tap William , Wexler Leonard H. , Antonescu Cristina R. 
Published: April 2024

PURPOSE Alterations of the NF1 tumor suppressor gene is the second most frequent genetic event in embryonal rhabdomyosarcoma (ERMS), but its associations with clinicopathologic features, outcome, or coexisting molecular events are not well defined. Additionally, NF1 alterations, mostly in the setting of neurofibromatosis type I (NF1), drive the pathogenesis of most malignant peripheral nerve sheath tumor with divergent RMS differentiation (also known as malignant triton tumor [MTT]). Distinguishing between these entities can be challenging because of their pathologic overlap. This study aims to comprehensively analyze the clinicopathologic and molecular spectrum of NF1-mutant RMS compared with NF1-associated MTT for a better understanding of their pathogenesis. METHODS We investigated the clinicopathologic and molecular landscape of a cohort of 22 NF1-mutant RMS and a control group of 13 NF1-associated MTT. Cases were tested on a matched tumor-normal hybridization capture-based targeted DNA next-generation sequencing. RESULTS Among the RMS group, all except one were ERMS, with a median age of 17 years while for MTT the mean age was 39 years. Three MTTs were misdiagnosed as ERMS, having clinical impact in one. The most frequent coexisting alteration in ERMS was TP53 abnormality (36%), being mutually exclusive from NRAS mutations (14%). MTT showed coexisting CDKN2A/B and PRC2 complex alterations in 38% cases and loss of H3K27me3 expression. Patients with NF1-mutant RMS exhibited a 70% 5-year survival rate, in contrast to MTT with a 33% 5-year survival. All metastatic NF1-mutant ERMS were associated with TP53 alterations. CONCLUSION Patients with NF1-mutant ERMS lacking TP53 alterations may benefit from dose-reduction chemotherapy. On the basis of the diagnostic challenges and significant treatment and prognostic differences, molecular profiling of challenging tumors with rhabdomyoblastic differentiation is recommended.
Insulinemic and Inflammatory Dietary Patterns Show Enhanced Predictive Potential for Gestational Diabetes Mellitus Risk
Authors: Zu Ping , Wang Haixia , Zhang Lei , Bian Haoran , Bian Jingfeng , Jiang Nan , Luo Wei , Xu Min , Xu Jirong , Jiang Xiaomin , Tao Ruixue , Zhu Peng 
Published: April 2024

Abstract

Context
The putative association between pro-inflammatory and hyperinsulinemic dietary patterns and susceptibility to gestational diabetes mellitus (GDM) remains unclear.

Objective
We aimed to compare the risk associated with the Mediterranean diet, as well as insulinemic and pro-inflammatory dietary patterns, in relation to the occurrence of GDM, and evaluate their predictive value.

Methods
We prospectively followed 8495 women from the Maternal and Infant Health cohort in Hefei, China (2015-2021). Using a food frequency questionnaire, we calculated the empirical dietary inflammatory pattern (EDIP), the empirical dietary index for hyperinsulinemia (EDIH) score, and the Mediterranean diet (MD) score. GDM was diagnosed based on a 2-hour 75-gram oral glucose tolerance test conducted between 24 to 28 weeks of gestation. Logistic regression was used to estimate the risk of GDM, while receiver operating characteristic (ROC) curves were constructed to evaluate the predictive performance of the empirical dietary index for GDM.

Results
Participants who followed hyperinsulinemic or pro-inflammatory dietary patterns to the greatest extent had a higher risk of developing GDM. The odds ratio (OR) for the highest quartile compared to the lowest quartile were 1.39 (95% CI, 1.30-1.49) for EDIH and 2.40 (95% CI, 1.88-3.01) for EDIP. The OR for the lowest quartile compared to the highest quartile was 1.33 (95% CI, 1.14-1.55) for MD. The ROC curve analysis indicated that the combination of EDIP and EDIH (AUC = 0.81; 95% CI, 0.78-0.82; P = .003) can effectively predict the occurrence of GDM.

Conclusion
Utilizing both empirical dietary indexes, EDIP and EDIH, might offer a potentially more effective approach in preventing GDM when compared to solely focusing on adherence to the MD pattern.
The BALB/c.mdx62 mouse exhibits a dystrophic muscle pathology and is a model of Duchenne muscular dystrophy
Authors: Swiderski Kristy , Chan Audrey S. , Herold Marco J. , Kueh Andrew J. , Chung Jin D. , Hardee Justin P. , Trieu Jennifer , Chee Annabel , Naim Timur , Gregorevic Paul , Lynch Gordon S. 
Published: April 2024

ABSTRACT
Duchenne muscular dystrophy (DMD) is a devastating monogenic skeletal muscle-wasting disorder. Although many pharmacological and genetic interventions have been reported in preclinical studies, few have progressed to clinical trials with meaningful benefit. Identifying therapeutic potential can be limited by availability of suitable preclinical mouse models. More rigorous testing across models with varied background strains and mutations can identify treatments for clinical success. Here, we report the generation of a DMD mouse model with a CRISPR-induced deletion within exon 62 of the dystrophin gene (Dmd) and the first generated in BALB/c mice. Analysis of mice at 3, 6 and 12 months of age confirmed loss of expression of the dystrophin protein isoform Dp427 and resultant dystrophic pathology in limb muscles and the diaphragm, with evidence of centrally nucleated fibers, increased inflammatory markers and fibrosis, progressive decline in muscle function, and compromised trabecular bone development. The BALB/c.mdx62 mouse is a novel model of DMD with associated variations in the immune response and muscle phenotype, compared with those of existing models. It represents an important addition to the preclinical model toolbox for developing therapeutic strategies.
Lower Limb Joint Mechanics during Maximal Accelerative and Decelerative Running
Authors: FITZWILLIAM EMILY , STEVENTON-LORENZEN NIKOLAI , OPAR DAVID , SCHACHE ANTHONY G. , MANIAR NIRAV 
Published: April 2024

ABSTRACT

Introduction
Maximal acceleration and deceleration tasks are frequently required in team sports, often occurring rapidly in response to external stimuli. Accelerating and decelerating can be associated with lower limb injuries; thus, knowledge of joint mechanics during these tasks can improve the understanding of both human high performance and injury mechanisms. The current study investigated the fundamental differences in lower limb joint mechanics when accelerating and decelerating by directly comparing the hip, knee, and ankle joint moments and work done between the two tasks.

Methods
Twenty participants performed maximal effort acceleration and deceleration trials, with three-dimensional marker trajectories and ground reaction forces collected simultaneously. Experimental data were combined with inverse dynamics analysis to compute joint moments and work.

Results
Net joint work for all lower limb joints was positive during acceleration and negative during deceleration. This occurred because of significantly greater positive work production from the ankle and hip during acceleration and significantly greater negative work production from all joints during deceleration. The largest contributions to positive work during acceleration came from the ankle, followed by the hip and knee joints, whereas the largest contributions to negative work during deceleration came from the knee and hip joints, followed by the ankle. Peak joint moments were significantly greater when decelerating compared with accelerating, except for the peak ankle plantarflexion and hip flexion moments, which were significantly greater when accelerating.

Conclusions
Our findings may help to guide training interventions, which aim to enhance the performance of acceleration and deceleration tasks, while also mitigating the associated injury risk.
Το ανθρώπινο πρόσωπο και το μετανθρώπινο όραμα: Θεολογική προσέγγιση
Authors: Δουραμάνη Γεωργία 
Published: April 2024

Η επιθυμία του ανθρώπου για μετεξέλιξή του σε ένα ον πιο προικισμένο προκειμένου να καλυτερεύσει τους όρους της ζωής του δεν είναι νέα, αλλά σύμφυτη με το είδος του. Αρωγοί στην πορεία του φιλοσοφία και θρησκεία, προσπαθώντας να δώσουν λύσεις στο διακαές αίτημα βελτίωσης της ανθρώπινης φύσης, από τη δική της θέση έκαστη. Φτάνοντας στην εποχή της Ύστερης -ή Ρευστής- Νεωτερικότητας ο άνθρωπος επιτυγχάνει να σπάσει τα δεσμά της παράδοσης, και στηριζόμενος στις δικές του δυνάμεις προωθεί το μοντέλο του ελεύθερου, αυτεξούσιου ανθρώπου.
Στην εποχή της αλματώδους προόδου της Επιστήμης και της Τεχνολογίας, ο άνθρωπος επαναπροσδιορίζει την ταυτότητά του ως πρόσωπο και τη συνολική του σχέση με τον Θεό και το περιβάλλον. Παράλληλα, ο ολιστικός μετασχηματισμός των δομικών στοιχείων που αποτέλεσαν τα θεμέλια της νεωτερικότητας συνιστά χαρακτηριστικό της μετάβασης σε μια μετα-μοντέρνα εποχή, δημιουργώντας νέα ταυτότητα για τον άνθρωπο, αυτή του μετα-ανθρώπου, ο οποίος δεν αποτελεί πλέον ένα ουτοπιστικό όραμα, παρά δρομολογείται μέσω του Διανθρωπισμού και των διεπαφών.
Στο πλαίσιο των νέων κοινωνικών μετασχηματισμών υπό το πρίσμα της τεχνολογικής προόδου, δημιουργείται προβληματισμός σχετικός με τη δυνατότητα διατήρησης της ταυτότητας του ανθρώπινου είδους ως «πρόσωπο» με τα χαρακτηριστικά του όντος-θεϊκού δημιουργήματος, βάσει της Πατερικής Διδασκαλίας, ή αν τελικά μετασχηματιστεί σε ναρκισσιστική, παντοδύναμη και αυτονομημένη ύπαρξη, απομακρυσμένη από την ανθρωπιά της.
Enfermedad pulmonar intersticial difusa de posible origen laboral atendida en el Servicio Navarro de Salud. Navarra, España, 2017-2022
Authors: García López Vega , Jové Gómez Dunia , Marin Martinez Belén , Chocrón Miño Sara , Castillo Sabogal Andrea , Ibarra García Angela 
Published: April 2024

Introducción: La enfermedad pulmonar intersticial difusa (EPID) describe un amplio grupo de trastornos con inflamación y fibrosis pulmonar. La asbestosis y la silicosis son las principales causas por exposición laboral. El objetivo de este trabajo fue estimar la proporción de casos de posible origen laboral y describir la exposición, situación clínica y laboral.
Método: Estudio longitudinal retrospectivo de los casos de EPID, en el período 2017-2022 en el Hospital Universitario de Navarra. Se completó la información con entrevista a los casos de posible origen laboral.
Resultados: De un total de 1067 casos de EPID, 56 tuvieron un posible origen laboral, 5,2% (3,9-6,6 IC 95%) 36 (64,3%) correspondieron a asbestosis, 15 (26,8%) a silicosis y 5 (8,9%) a neumoconiosis no especificada. Las actividades más frecuentes en silicosis fueron “corte-tallado de piedra” y para asbestosis ”fabricación productos hierro”. La media de edad de los casos de asbestosis fue superior a los de silicosis (78,2 vs. 67,3 años), así como su afectación clínica. Cinco casos (8,9%) habían sido reconocidos como enfermedad profesional
Conclusiones: La implementación de una herramienta informática en historia clínica ha hecho posible estimar la magnitud y valorar la evolución de las EPID laborales atendidas en el servicio nacional de salud. Las actividades económicas reflejan la estructura económica de riesgo de la región. Sin embargo, existe una falta de su reconocimiento como enfermedad profesional y suponen una carga de enfermedad respiratoria evitable.
Tuberculosis: Implications for Dentistry
Authors: Chong Xue-Mei , McClean Lucy , McMaster Paddy 
Published: April 2024

Tuberculosis (TB) is an airborne infection caused by Mycobacterium tuberculosis complex and is highly infectious. Therefore, precautionary measures should be implemented prior to dental treatment to reduce the risk of infection to staff and other patients. Dental history and examination have a role in identification of TB infection. Complex medical history poses a challenge to safe patient management in a dental setting, and close liaison between different disciplines in managing these patients is of paramount importance. This article explores the classical clinical features of pulmonary TB, its oral manifestations and the dental management of patients with active and latent TB. CPD/Clinical Relevance: Clinicians should be aware of the clinical features and dental management of patients with active and latent TB.
HDACi vorinostat protects muscle from degeneration after acute rotator cuff injury in mice
Authors: Gil-Melgosa Lara , Llombart-Blanco Rafael , Extramiana Leire , Lacave Isabel , Abizanda Gloria , Miranda Estibaliz , Agirre Xabier , Prósper Felipe , Pineda-Lucena Antonio , Pons-Villanueva Juan , Pérez-Ruiz Ana 
Published: April 2024

AimsRotator cuff (RC) injuries are characterized by tendon rupture, muscle atrophy, retraction, and fatty infiltration, which increase injury severity and jeopardize adequate tendon repair. Epigenetic drugs, such as histone deacetylase inhibitors (HDACis), possess the capacity to redefine the molecular signature of cells, and they may have the potential to inhibit the transformation of the fibro-adipogenic progenitors (FAPs) within the skeletal muscle into adipocyte-like cells, concurrently enhancing the myogenic potential of the satellite cells.MethodsHDACis were added to FAPs and satellite cell cultures isolated from mice. The HDACi vorinostat was additionally administered into a RC injury animal model. Histological analysis was carried out on the isolated supra- and infraspinatus muscles to assess vorinostat anti-muscle degeneration potential.ResultsVorinostat, a HDACi compound, blocked the adipogenic transformation of muscle-associated FAPs in culture, promoting myogenic progression of the satellite cells. Furthermore, it protected muscle from degeneration after acute RC in mice in the earlier muscle degenerative stage after tenotomy.ConclusionThe HDACi vorinostat may be a candidate to prevent early muscular degeneration after RC injury.Cite this article: Bone Joint Res 2024;13(4):169–183.
Helminth exposure and immune response to the two-dose heterologous Ad26.ZEBOV, MVA-BN-Filo Ebola vaccine regimen
Authors: Barry Houreratou , Lhomme Edouard , Surénaud Mathieu , Nouctara Moumini , Robinson Cynthia , Bockstal Viki , Valea Innocent , Somda Serge , Tinto Halidou , Meda Nicolas , Greenwood Brian , Thiébaut Rodolphe , Lacabaratz Christine 
Published: April 2024

Background
The exposure to parasites may influence the immune response to vaccines in endemic African countries. In this study, we aimed to assess the association between helminth exposure to the most prevalent parasitic infections, schistosomiasis, soil transmitted helminths infection and filariasis, and the Ebola virus glycoprotein (EBOV GP) antibody concentration in response to vaccination with the Ad26.ZEBOV, MVA-BN-Filo vaccine regimen in African and European participants using samples obtained from three international clinical trials.

Methods/Principal findings
We conducted a study in a subset of participants in the EBL2001, EBL2002 and EBL3001 clinical trials that evaluated the Ad26.ZEBOV, MVA-BN-Filo vaccine regimen against EVD in children, adolescents and adults from the United Kingdom, France, Burkina Faso, Cote d’Ivoire, Kenya, Uganda and Sierra Leone. Immune markers of helminth exposure at baseline were evaluated by ELISA with three commercial kits which detect IgG antibodies against schistosome, filarial and Strongyloides antigens. Luminex technology was used to measure inflammatory and activation markers, and Th1/Th2/Th17 cytokines at baseline. The association between binding IgG antibodies specific to EBOV GP (measured on day 21 post-dose 2 and on Day 365 after the first dose respectively), and helminth exposure at baseline was evaluated using a multivariable linear regression model adjusted for age and study group.
Seventy-eight (21.3%) of the 367 participants included in the study had at least one helminth positive ELISA test at baseline, with differences of prevalence between studies and an increased prevalence with age. The most frequently detected antibodies were those to Schistosoma mansoni (10.9%), followed by Acanthocheilonema viteae (9%) and then Strongyloides ratti (7.9%). Among the 41 immunological analytes tested, five were significantly (p < .003) lower in participants with at least one positive helminth ELISA test result: CCL2/MCP1, FGFbasic, IL-7, IL-13 and CCL11/Eotaxin compared to participants with negative helminth ELISA tests. No significant association was found with EBOV-GP specific antibody concentration at 21 days post-dose 2, or at 365 days post-dose 1, adjusted for age group, study, and the presence of any helminth antibodies at baseline.

Conclusions/Significance
No clear association was found between immune markers of helminth exposure as measured by ELISA and post-vaccination response to the Ebola Ad26.ZEBOV/ MVA-BN-Filo vaccine regimen.

Trial registration
NCT02416453, NCT02564523, NCT02509494. ClinicalTrials.gov.
Building consensus on priority areas for Sub-Saharan Africa’s ageing population research: An e-Delphi study protocol
Authors: Okoh Augustine Chukwuebuka , Onyeso Ogochukwu Kelechi , Ekemezie Wendy , Oyinlola Oluwagbemiga , Akinrolie Olayinka , Kalu Michael ,   on behalf of the Emerging Researchers & Professionals in Ageing-African Network
Published: April 2024

Background
Improvement in medico-social services has increased life expectancy and population ageing in Sub-Saharan Africa (SSA). It was estimated that about 163 million people aged 65 and older will be resident in SSA by 2050. There is inadequate ageing research capacity in SSA which necessitates this study to (a) identify a decade-long ageing research opportunities, challenges, and solutions, and (b) prioritize critical ageing research areas and methodologies relevant to the SSA.

Methods
We designed an e-Delphi protocol following the Reporting Guideline for Priority Setting of Health Research with Stakeholder. The stakeholders will be researchers, practitioners, older adults, and caregivers purposively selected through snowballing quota sampling to complete three rounds of e-Delphi surveys. Round 1 will involve open-ended questions derived from the study objectives. Responses from round 1 will be prepared as a checklist for stakeholders to rate during rounds 2 & 3, using a 9-point scale: low priority (1–3), moderate priority (4–6), and high priority (7–9). The criterion for reaching a consensus will be ≥ 70% of stakeholders rating an item “high priority” and ≤ 15% as “low priority.” Quantitative data will be analysed using descriptive statistics, Wilcoxon matched-pairs signed-rank test will be used to assess the stability of stakeholders’ responses, and qualitative comments will be analysed using content analysis.

Discussion and implications
Setting aging research/practice priorities will help maximize the benefits of research investment and provide valuable direction for allocating public and private research funds to areas of strategic importance.
Transcriptome analysis reveals the mechanism of NaHCO3 promoting tobacco leaf maturation
Authors: Wang Tingting , Zhao Yuanyuan , Wang Dexun , Shi Hongzhi 
Published: April 2024

Abstract
NaHCO3 accelerates the aging of tobacco leaves; however, the underlying molecular mechanisms have not been elucidated. This study aimed to explore the mechanism of NaHCO3 in the promotion of tobacco leaf maturation using transcriptome analysis. Leaves on plants or detached leaves of the tobacco variety, Honghua Dajinyuan, were sprayed with or without 1% NaHCO3. The leaf yellowing was observed, the pigment content and enzyme activities were determined and RNA sequencing (RNA-seq) was performed. Spraying NaHCO3 onto detached leaves was found to promote leaf yellowing. Pigment content, catalase activity, and superoxide dismutase activity significantly decreased, whereas peroxidase activity and malondialdehyde content significantly increased. RNA-seq demonstrated that spraying with NaHCO3 upregulated genes associated with cysteine and methionine metabolism; alpha-linolenic acid metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis and downregulated genes related to photosynthesis and carotenoid biosynthesis. Genes correlated with autophagy-other, valine, leucine, and isoleucine degradation, and the MAPK signaling pathway were upregulated while those correlated with DNA replication, phenylalanine, and tyrosine and tryptophan biosynthesis were downregulated in detached leaves sprayed with NaHCO3 compared with the plant leaves sprayed with NaHCO3. Overall, this study is the first to elucidate the molecular and metabolic mechanisms of NaHCO3 in the promotion of tobacco leaf maturation.