bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytotoxic response of tumor-infiltrating lymphocytes of head and neck cancer slice cultures under mitochondrial dysfunction
Authors: Greier Maria do Carmo , Runge Annette , Dudas Jozsef , Hartl Roland , Santer Matthias , Dejaco Daniel , Steinbichler Teresa Bernadette , Federspiel Julia , Seifarth Christof , Konschake Marko , Sprung Susanne , Sopper Sieghart , Randhawa Avneet , Mayr Melissa , Hofauer Benedikt Gabriel , Riechelmann Herbert 
Published: March 2024

BackgroundHead and neck squamous cell carcinomas (HNSCC) are highly heterogeneous tumors. In the harsh tumor microenvironment (TME), metabolic reprogramming and mitochondrial dysfunction may lead to immunosuppressive phenotypes. Aerobic glycolysis is needed for the activation of cytotoxic T-cells and the absence of glucose may hamper the full effector functions of cytotoxic T-cells. To test the effect of mitochondrial dysfunction on cytotoxic T cell function, slice cultures (SC) of HNSCC cancer were cultivated under different metabolic conditions.MethodsTumor samples from 21 patients with HNSCC were collected, from which, SC were established and cultivated under six different conditions. These conditions included high glucose, T cell stimulation, and temporarily induced mitochondrial dysfunction (MitoDys) using FCCP and oligomycin A with or without additional T cell stimulation, high glucose and finally, a control medium. Over three days of cultivation, sequential T cell stimulation and MitoDys treatments were performed. Supernatant was collected, and SC were fixed and embedded. Granzyme B was measured in the supernatant and in the SC via immunohistochemistry (IHC). Staining of PD1, CD8/Ki67, and cleaved­caspase­3 (CC3) were performed in SC.ResultsHematoxylin eosin stains showed that overall SC quality remained stable over 3 days of cultivation. T cell stimulation, both alone and combined with MitoDys, led to significantly increased granzyme levels in SC and in supernatant. Apoptosis following T cell stimulation was observed in tumor and stroma. Mitochondrial dysfunction alone increased apoptosis in tumor cell aggregates. High glucose concentration alone had no impact on T cell activity and apoptosis. Apoptosis rates were significantly lower under conditions with high glucose and MitoDys (p=0.03).ConclusionStimulation of tumor-infiltrating lymphocytes in SC was feasible, which led to increased apoptosis in tumor cells. Induced mitochondrial dysfunction did not play a significant role in the activation and function of TILs in SC of HNSCC. Moreover, high glucose concentration did not promote cytotoxic T cell activity in HNSCC SC.
A 6-week randomized-controlled field study: effect of isokinetic eccentric resistance training on strength, flexibility and muscle structure of the shoulder external rotators in male junior handball players
Authors: Vetter Sebastian , Witt Maren , Hepp Pierre , Schleichardt Axel , Schleifenbaum Stefan , Roth Christian , Denecke Timm , Henkelmann Jeanette , Köhler Hans-Peter 
Published: March 2024

Background: Team handball involves a tremendous amount of shoulder motion with high forces during repeated extended external range of motion. This causes shoulder complaints and overuse injuries. While eccentric training for the lower extremity shows preventive effects by improving strength, range of motion and fascicle length, there is a research gap for the shoulder joint and for advanced tissue characterization using diffusion tensor imaging.Objectives: To investigate the effects of 6-week eccentric isokinetic resistance training on strength, flexibility, and fiber architecture characteristics of the external rotators compared to an active control group in junior male handball players.Methods: 15 subjects were randomly assigned to the eccentric training group and 14 subjects to the active control group (conventional preventive training). Primary outcome measures were eccentric and concentric isokinetic strength of the external rotators, range of motion, and muscle fascicle length and fascicle volume.Results: The intervention group, showed significant changes in eccentric strength (+15%). The supraspinatus and infraspinatus muscles showed significant increases in fascicle length (+13% and +8%), and in fractional anisotropy (+9% and +6%), which were significantly different from the control group.Conclusion: Eccentric isokinetic training has a significant effect on the function and macroscopic structure of the shoulder external rotators in male junior handball players. While strength parameters and muscle structure improved, range of motion did not change. This research helps understanding the physiology of muscle and the role of eccentric training on shoulder function and muscle structure. Furthermore, DTI was found to be a promising tool for advanced tissue characterization, and the in vivo derived data can also serve as model input variables and as a possibility to extend existing ex-vivo muscle models. Future research is needed for functional and structural changes following convenient eccentric field exercises.
Genetic gains in forage sorghum for adaptive traits for non - conventional area through multi-trait-based stability selection methods
Authors: Behera Partha Pratim , Singode Avinash , Bhat B. Venkatesh , Ronda Venkateswarlu , Borah Nayanmoni , Verma Harendra , Gogoi Labhya Rani , Borah Jyoti Lekha , Majhi Prasanta Kumar , Saharia Niharika , Sarma Ramendra Nath 
Published: March 2024

IntroductionIn the Asian tropics, unpredictable weather increases the risk of abiotic stresses in sorghum areas, making it harder to meet predicted demand. Genotype-by environment interaction (GEI) and the lack of an effective multi-trait-based selection approach make it challenging to breed climateresilient forage sorghum that adapts to nonconventional areas.MethodsThe present investigation carried out to estimate genetic parameters, inter trait associations, genetic gain under selection (SGs) of 95 diverse forage sorghum genotypes. Fourteen forage yield and other secondary traits were evaluated at five different growing seasons at two locations. Negative and positive genetic gains under selection were estimated across different growing seasons including Kharif, Rabi and Summer in the year 2020 and 2021.Results and discussionThe GEI effects were significant (P &lt; 0.001) for all the studied traits. The multi trait based stability indices have been said to assist breeders in ensuring sustained progress in primary traits likeforage yield without sacrificing genetic advancement in secondary traits. Fourteen genotypes were selected through each evaluation methods including genotype – ideotype distance index (MGIDI), multi-trait stability index (MTSI), multi-trait stability and mean performance (MTMPS) and multi-trait index based on factor analysis and genotype-ideotype distance (FAIBLUP Index), assuming 15% selection intensity. According to MGIDI, the selected genotypes exhibited desired positive genetic gains for dry forage yield per plant, inter-nodal length, green forage yield per plant, and plant height and negative genetic gains for days to 50% flowering. The strength and weakness plot is a potential graphical tool as portrayed by MGIDI, to identify and develop desirable genotype for particular environment. Two genotypes, G36 (302B) and G89 (348B) were found to be common across all four evaluation methods based on all the studied traits.BackgroundMulti-trait stability evaluation approaches are reliable and accessible for selecting multiple traits under varied testing environments with low multicollinearity issues. These tools proved effective in enhancing selection strategies and optimising breeding schemes for the development of climate-resilient forage sorghum genotypes. The aforementioned genotypes were found to be the most reliable, high-yielding, and earlymaturing and could be suggested for variety and hybrid development and ideotype breeding programmes to ensure the food and nutritional security.
Brandt’s vole hole detection and counting method based on deep learning and unmanned aircraft system
Authors: Wu Wei , Liu Shengping , Zhong Xiaochun , Liu Xiaohui , Wang Dawei , Lin Kejian 
Published: March 2024

Rodents are essential to the balance of the grassland ecosystem, but their population outbreak can cause major economic and ecological damage. Rodent monitoring is crucial for its scientific management, but traditional methods heavily depend on manual labor and are difficult to be carried out on a large scale. In this study, we used UAS to collect high–resolution RGB images of steppes in Inner Mongolia, China in the spring, and used various object detection algorithms to identify the holes of Brandt’s vole (Lasiopodomys brandtii). Optimizing the model by adjusting evaluation metrics, specifically, replacing classification strategy metrics such as precision, recall, and F1 score with regression strategy-related metrics FPPI, MR, and MAPE to determine the optimal threshold parameters for IOU and confidence. Then, we mapped the distribution of vole holes in the study area using position data derived from the optimized model. Results showed that the best resolution of UAS acquisition was 0.4 cm pixel–1, and the improved labeling method improved the detection accuracy of the model. The FCOS model had the highest comprehensive evaluation, and an R2 of 0.9106, RMSE of 5.5909, and MAPE of 8.27%. The final accuracy of vole hole counting in the stitched orthophoto was 90.20%. Our work has demonstrated that UAS was able to accurately estimate the population of grassland rodents at an appropriate resolution. Given that the population distribution we focus on is important for a wide variety of species, our work illustrates a general remote sensing approach for mapping and monitoring rodent damage across broad landscapes for studies of grassland ecological balance, vegetation conservation, and land management.
Organ-level distribution tandem mass spectrometry analysis of three structural types of brassinosteroids in rapeseed
Authors: Tong Jianhua , Zhao Wenkui , Wang Keming , Deng Danyi , Xiao Langtao 
Published: March 2024

BackgroundBrassinosteroids (BRs) are a class of naturally occurring steroidal phytohormones mediating a wide range of pivotal developmental and physiological functions throughout the plant’s life cycle. Therefore, it is of great significance to determine the content and the distribution of BRs in plants.Regretfully, although a large number of quantitative methods for BRs by liquid chromatography-tandem mass spectrometry (LC-MS/MS) have been reported, the in planta distribution of BRs is still unclear because of their lower contents in plant tissues and the lack of effective ionizable groups in their chemical structures.MethodsWe stablished a novel analytical method of BRs based on C18 cartridge solid-phase extraction (SPE) purification, 4-(dimethylamino)-phenylboronic acid (DMAPBA) derivatization, and online valve-switching system coupled with ultra-high performance liquid chromatography-electro spray ionization-triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS). This method has been used to quantify three structural types of BRs (epibrassinolide, epicastasterone, and 6-deoxo-24-epicastaster one) in different organs of Brassica napus L. (rapeseed).ResultsWe obtained the contents of three structural types of BRs in various organ tissues of rapeseed. The contents of three BRs in rapeseed flowers were the highest, followed by tender pods. The levels of three BRs all decreased during the maturation of the organs. We outlined the spatial distribution maps of three BRs in rapeseed based on these results, so as to understand the spatial distribution of BRs at the visual level.ConclusionsOur results provided useful information for the precise in situ localization of BRs in plants and the metabolomic research of BRs in future work. The in planta spatial distribution of BRs at the visual level has been studied for the first time.
Validation of the PL-C Quest in China: understanding the pictorial physical literacy self-report scale
Authors: Wu Yu , Wang Xinxiang , Wang Hongbiao , Wang Lijuan , Tian Ying , Ji Zhiguang , Wang Liyan 
Published: March 2024

IntroductionThe notion of physical literacy is gaining interest from several countries as a potential mechanism for understanding the development of the physical self. This research endeavor represents an inaugural attempt to translate the Australian Physical Literacy Questionnaire for Children (PL-C Quest) into Chinese to evaluate the reliability and validity of the Chinese version of the PL-C Quest to assess physical literacy among children in mainland China.MethodsThe Beaton translation paradigm was used to carry out language translation, back-translation, cultural adaptation, and presurveys. Data were collected from 642 children aged 6–12 years, with a mean age of 9.71 years (SD 1.816), to test the reliability of the Chinese version of the PL-C Quest.ResultsThe PL-C Quest items translated well (6.187 ~ 15.499) and correlated well (0.441 ~ 0.622). The Chinese version of the PL-C Quest had good reliability, with retest reliability values ranging from 0.91 to 0.74, Cronbach’s alpha from 0.65 to 0.894, and McDonald’s ω from the Spearman-Brown Coefficient was 0.84. The validity results are acceptable because the CFI, IFI, and TLI values are above 0.8 and close to 0.9, but the model fit’s chi-square degrees-of-freedom ratio of 2.299, the RMSEA of 0.05, which was less than 0.08.DiscussionAfter translation and cultural adaptation, the Chinese version of the PL-C Quest is a reliable measurement tool and can be used in the Chinese region.
Anxiety in Polish adult patients with inborn errors of immunity: a cross-sectional study
Authors: Grochowalska Kinga , Ziętkiewicz Marcin , Nowicka-Sauer Katarzyna , Topolski Mariusz , Więsik-Szewczyk Ewa , Matyja-Bednarczyk Aleksandra , Napiórkowska-Baran Katarzyna , Zdrojewski Zbigniew 
Published: March 2024

BackgroundPatients with inborn errors of immunity (IEI) experience recurrent infections, autoimmunity, and malignancies. Owing to repeated medical procedures, the need for constant treatment and surveillance, and the unpredictable course of the disease, patients with IEI are prone to develop mental health disorders, including anxiety. In this study, we aimed to assess the prevalence and level of anxiety symptoms in adult Polish patients with IEI and explore the determinants of anxiety in this group of patients.MethodsData from 105 Polish patients with IEI were collected via the hospital anxiety and depression scale (HADS), brief illness perception questionnaire (B-IPQ), illness cognition questionnaire (ICQ), Pittsburgh sleep quality index (PSQI), and a questionnaire on general health and demographic data. For statistical analyses of data, the normality of distribution of quantitative data was assessed, and internal consistency of tests was investigated using Cronbach’s alpha coefficient; moreover, we performed the analysis of correlations and between-group differences, and path analysis to explore causal relationships. Significance was considered at p &lt; 0.050.ResultsThirty-eight (36.2%) patients had anxiety symptoms (HADS-A ≥ 8); 14 (13.3%) patients had severe anxiety (score ≥ 11), and 24 (22.9%) had moderate anxiety (score of 8–10). Patients with poor sleep quality, higher pain frequency, younger age, and no fixed income had higher anxiety scores than others. Emotional and cognitive representations of illness were positively correlated with anxiety levels. Intense anxiety was related to more negative illness perception, higher helplessness, lower illness acceptance, and lower perceived benefits.DiscussionAnxiety is common in patients with IEI. However, results indicate that it is not related to a more severe course of IEI or several comorbidities, whereas, pain frequency and poor sleep quality were identified to be important clinical factors for anxiety. Because anxiety was related to negative illness perception, psychological therapy may apply to this group of patients.
The prevalence and sociodemographic determinants of tobacco and nicotine use among students in healthcare disciplines in Saudi Arabian universities: a cross-sectional survey
Authors: Aldhahir Abdulelah M. , Siraj Rayan A. , Alqarni Abdullah A. , Alqahtani Jaber S. , Alyami Mohammed M. , Majrshi Mansour S. , Alwafi Hassan , Alqahtani Mohammed M. , Monshi Sarah S. , Al-Zalabani Abdulmohsen H. , Alanazi Abdullah M. 
Published: March 2024

BackgroundTobacco smoking is one of the most significant health-related problems worldwide. However, the prevalence and sociodemographic determinants of tobacco and nicotine use among students on healthcare courses in Saudi Arabia are limited.MethodsThis cross-sectional study used a questionnaire that was distributed via SurveyMonkey between November 2022 and June 2023 to all student universities offering healthcare courses. Data are presented as frequency and percentages. The associated sociodemographic factors with current tobacco and nicotine use were subjected to logistic regression.ResultsOverall, 5,012, of whom 3,872 (77.25%) were males, responded to the online survey. The prevalence of current tobacco and nicotine use was 3,553 (71%). The majority of users used electronic nicotine delivery systems (1,622: 32.36%). The current use of cigarettes (AOR: 1.75 (1.42 to 2.15)), e-cigarettes (AOR: 1.17 (1.01 to 1.37)), and smokeless tobacco substances (AOR: 1.35 (1.02 to 1.90)) were more pronounced among males compared to female students. However, the current use of a hookah was less among males (AOR: 0.74 (95% CI: 61 to 0.91)). Smoking cigarettes was practiced less among students living in other regions compared to the Central Region. However, smokeless tobacco substances seem to be more prevalent in the Western, Southern, and Northern Regions, (AOR: 1.57 (95% CI: 1.09 to 2.26)), (AOR: 1.43 (95% CI: 1.04 to 1.95)), and (AOR: 1.57 (95% CI: 1.09 to 2.26)), respectively.ConclusionSmoking is prevalent among students in the healthcare disciplines, with electronic nicotine delivery systems being the most used product. Several sociodemographic factors were associated with higher tobacco or nicotine use.
Isolation of feline islets of Langerhans by selective osmotic shock produces glucose responsive islets
Authors: Porter Lauren T. , Adin Christopher A. , Crews Chiquitha D. , Mott Jocelyn , Gilor Chen 
Published: March 2024

IntroductionPancreatic islet isolation is essential for studying islet physiology, pathology, and transplantation, and feline islets could be an important model for human type II diabetes mellitus (T2D). Traditional isolation methods utilizing collagenases inflict damage and, in cats, may contribute to the difficulty in generating functional islets, as demonstrated by glucose-stimulated insulin secretion (GSIS). GLUT2 expression in β cells may allow for adaptation to hyperosmolar glucose solutions while exocrine tissue is selectively disrupted.MethodsHere we developed a protocol for selective osmotic shock (SOS) for feline islet isolation and evaluated the effect of different hyperosmolar glucose concentrations (300 mmol/L and 600 mmol/L) and incubation times (20 min and 40 min) on purity, morphology, yield, and GSIS.ResultsAcross protocol treatments, islet yield was moderate and morphology excellent. The treatment of 600 mmol/L glucose solution with 20 min incubation resulted in the highest stimulation index by GSIS.DiscussionGlucose responsiveness was demonstrated, permitting future in vitro studies. This research opens avenues for understanding feline islet function and transplantation possibilities and enables an additional islet model for T2D.
Handbook Sketching Out Vibrant Debates on Queer and Trans Feminisms in Performance
Authors: Suneson Ellen 
Published: March 2024

Rosenberg, Tiina, Sandra D'Urso & Anna Renée Winget, eds. 2021. The Palgrave Handbook of Queer and Trans Feminisms in Contemporary Performance. Cham: Palgrave Macmillan. (541 pages)
Efektifitas penggunaan media video animasi tentang anemia pada remaja putri
Authors: Safitri Evita Dwi , Aritonang Irianton , Wirawan Susilo , Sitasari Almira 
Published: March 2024

Latar Belakang: Anemia adalah penyakit yang disebabkan oleh kekurangan asupan zat besi dalam tubuh yang sangat berperan dalam membentuk hemoglobin. Remaja putri merupakan salah satu kelompok yang rentan terhadap anemia. Tujuan: Mengetahui efektivitas media video animasi tentang anemia dalam meningkatkan pengetahuan dan sikap pada remaja putri SMA di wilayah Sleman. Metode: Penelitian ini adalah quasy experiment selama dua minggu dengan menggunakan pretest and posttest with control group design. Subjek penelitian adalah siswa remaja putri kelas XI MIPA sebanyak 30 siswa. Variabel yang diteliti adalah pengetahuan dan sikap pada kelompok eksperimen (menggunakan video animasi) dan kelompok kontrol (menggunakan leaflet) berdasarkan skor pretest dan posttest. Analisis data menggunakan uji Independent Sample T-test dan uji Paired Sample T-test. Hasil: Terjadi kenaikan skor pengetahuan remaja putri pada kelompok eksperimen sebesar 25,22 dan kelompok kontrol sebesar 20. Demikian pula terjadi kenaikan skor sikap remaja putri pada kelompok eksperimen sebesar 15,94 dan kelompok kontrol sebesar 8,8. Ada perbedaan yang signifikan pada peningkatan pengetahuan dan sikap remaja putri kelompok eksperimen dan kelompok kontrol (p<0,005). Kesimpulan: Media video animasi lebih efektif dalam meningkatkan pengetahuan dan sikap tentang anemia pada siswa remaja putri. Saran: Media tersebut dapat diterapkan untuk penggunaan media pada kegiatan penyuluhan atau konseling di sekolah.
PENGARUH MUTU PELAYANAN TERHADAP TINGKAT KEPUASAN PASIEN RAWAT JALAN
Authors: Al Rajab Muhammad , Andilah Sultan 
Published: March 2024

Latar belakang: Kepuasan pelanggan mempunyai tempat tersendiri dan merupakan hal yang sangat penting untuk bertahannya suatu rumah sakit. Kepuasan akan terjadi apabila harapan dari pelanggan dapat terpenuhi oleh pelayanan yang diberikan rumah sakit untuk dapat memanfaatkan kembali layanan kesehatan. Tujuan: untuk mengetahui pengaruh mutu pelayanan terhadap tingkat Kepuasan Pasien Rawat Jalan di Rumah Sakit Umum Bahtermas Tahun 2022. Metode: Penelitian ini merupakan penelitian analitik kuantitatif dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah pasien rawat jalan bahteramas tahun 2021. Teknik sampling dalam penelitian ini menggunakan cara Probability Sampling. Penelitian ini dilakukan pada bulan Oktober s/d November 2022. Teknik analisa data bivariat menggunakan Chi Square. Hasil: Terdapat hubungan daya tanggap (Responsiveness) terhadap kepuasan pasien dengan nilai p sebesar 0.046. Terdapat hubungan bukti fisik (Tangible terhadap kepuasan pasien dengan nilai p sebesar 0.025. Terdapat hubungan Kehandalan (Reliability) terhadap kepuasan pasien dengan nilai p sebesar 0.016. Terdapat hubungan Asurance (Jaminan) terhadap kepuasan pasien dengan nilai p sebesar 0.015. Tidak terdapat hubungan hubungan Empathy (Empati) terhadap kepuasan pasien dengan nilai p sebesar 0.185. Kesimpulan Terdapat hubungan daya tanggap (Responsiveness), bukti fisik (Tangible), Kehandalan (Reliability), Asurance (Jaminan) terhadap kepuasan pasien dan Tidak terdapat hubungan hubungan Empathy (Empati) terhadap kepuasan.