bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthetic Inhibitors of Extended Helix–Protein Interactions Based on a Biphenyl 4,4′‐Dicarboxamide Scaffold
Authors: Rodriguez Johanna M. , Nevola Laura , Ross Nathan T. , Lee Gui‐in , Hamilton Andrew D. 
Published: March 2009

AbstractTurn Bak: We present rationally designed scaffolds that mimic the spatial projection of the i, i+4, i+7, and i+11 residues of an α‐helix. A library of biphenyl derivatives was shown by competition fluorescence polarization and ITC to mimic Bak and disrupt the Bak/Bcl‐xL protein–protein interaction. 15N HSQC experiments confirmed that the surface of Bcl‐xL normally occupied by Bak was the target area of our new synthetic inhibitors.magnified image
Cyclic Peptide–Polymer Complexes and Their Self‐Assembly
Authors: Bélanger Dominique , Tong Xia , Soumaré Sadia , Dory Yves L. , Zhao Yue 
Published: March 2009

AbstractPepped up polymers: The synthesis and properties of novel chiral cyclic peptides designed to complex with suitable polymers through hydrogen bonding are described. A substituted cyclic peptide self‐assembles into supramolecular nanotubes and develops noncovalent interactions with poly(vinyl alcohol) (PVA) by means of its carboxyl side chains.magnified imageThe efficient synthesis of novel chiral cyclic peptides cyclo[NHCHX‐CHCHCH2CO(NHCH2CHCHCH2CO)2] designed to develop hydrogen‐bonding interactions with suitable polymers is described. Complexation of a carboxylic acid derivatized cyclic peptide 2 (X=CH2OCOCH2CH2CO2H) capable of self‐assembling as “endless” tubes, with poly(vinyl alcohol) (PVA) led to a vast weak‐interaction network, in which the cyclopeptide developed extensive hydrogen‐bonding interactions with the hydroxyl groups of PVA through not only the carboxylic acid, but also its ester carbonyl and amide groups. In aqueous solution, the peptide/PVA complexes self‐assemble into long‐grain ricelike aggregates compatible with the stacking of cyclic peptides through intercycle hydrogen bonds. Upon casting on silicon wafer, the anisotropic aggregates can coalesce to form filaments tens of micrometers long. The study demonstrates that complexing functionalized cyclic peptides with polymers through hydrogen bonding is a useful approach for using polymers to mediate the self‐assembly and self‐organization of cyclic peptides.
Bilaterally propagating waves of spontaneous activity arising from discrete pacemakers in the neonatal mouse cerebral cortex
Authors: Lischalk Jonathan W. , Easton Curtis R. , Moody William J. 
Published: March 2009

AbstractSpontaneous electrical activity that moves in synchronized waves across large populations of neurons plays widespread and important roles in nervous system development. The propagation patterns of such waves can encode the spatial location of neurons to their downstream targets and strengthen synaptic connections in coherent spatial patterns. Such waves can arise as an emergent property of mutually excitatory neural networks, or can be driven by a discrete pacemaker. In the mouse cerebral cortex, spontaneous synchronized activity occurs for approximately 72 h of development centered on the day of birth. It is not known whether this activity is driven by a discrete pacemaker or occurs as an emergent network property. Here we show that this activity propagates as a wave that is initiated at either of two homologous pacemakers in the temporal region, and then propagates rapidly across both sides of the brain. When these regions of origin are surgically isolated, waves do not occur. Therefore, this cortical spontaneous activity is a bilateral wave that originates from a discrete subset of pacemaker neurons. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009
Delayed axonal pruning in the ant brain: A study of developmental trajectories
Authors: Seid Marc A. , Wehner Rüdiger 
Published: March 2009

AbstractThe coordination of neuronal maturation and behavioral development is a vital component of survival. The degradation of excessive axonal processes and neuronal networks is a ubiquitous developmental process. In Drosophila, a great portion of axonal pruning occurs during metamorphosis and transpires within hours after pupation. In contrast, we show, using EM‐serial sectioning and 3D‐reconstructions, that axonal pruning occurs after eclosion and over the course of 60 days in Cataglyphis albicans. Using the mushroom bodies of the brains of Cataglyphis, which have well‐developed lip (olfactory integrator) and collar (visual integrator) regions, we show that axonal pruning is dependent upon the differences in the developmental trajectory of the lip and the collar brain regions and happens after eclosion. The elimination of the axonal boutons is most delayed in the collar region, where it is postponed until the ant has had extensive visual experience. We found that individual brain components within a single neuropil can develop at different rates that correlate with the behavioral ecology of these ants and suggest that glia may be mediating the axonal pruning. Our study provides evidence that adult ants may have relatively neotenous brains, and thus more flexibility, allowing them to neuronally adapt to the environment. This neoteny may, in part, explain the neural basis for age‐dependent division of labor and the amazing behavioral flexibility exhibited by ants. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009
Notch activation induces neurite remodeling and functional modifications in SH‐SY5Y neuronal cells
Authors: Ferrari‐Toninelli Giulia , Bonini Sara Anna , Uberti Daniela , Napolitano Francesco , Stante Maria , Santoro Federica , Minopoli Giuseppina , Zambrano Nicola , Russo Tommaso , Memo Maurizio 
Published: March 2009

AbstractNotch proteins are definitely recognized as key regulators of the neuronal fate during embryo development, but their function in the adult brain is still largely unknown. We have previously demonstrated that Notch pathway stimulation increases microtubules stability followed by the remodeling of neuronal morphology with neurite varicosities loss, thicker neuritis, and enlarged growth cones. Here we show that the neurite remodeling is a dynamic event, dependent on transcription and translation, and with functional implications. Exposure of differentiated human SH‐SY5Y neuroblastoma cells to the Notch ligand Jagged1 induces varicosities loss all along the neurites, accompanied by the redistribution of presynaptic vesicles and the decrease in neurotransmitters release. As evaluated by time lapse digital imaging, dynamic changes in neurite morphology were rapidly reversible and dependent on the activation of the Notch signaling pathway. In fact, it was prevented by the inhibition of the proteolytic γ‐secretase enzyme or the transcription machinery, and was mimicked by the transfection of the intracellular domain of Notch. One hour after treatment with Jagged1, several genes were downregulated. Many of these genes encode proteins that are known to be involved in protein synthesis. These data suggest that in adult neurons, Notch pathway activates a transcriptional program that regulates the equilibrium between varicosities formation and varicosities loss in the neuronal presynaptic compartment involving the expression and redistribution of both structural and functional proteins. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009
New Naphthalene‐Chroman Coupling Products from the Endophytic Fungus, Nodulisporium sp. from Erica arborea
Authors: Dai Jingqiu , Krohn Karsten , Draeger Siegfried , Schulz Barbara 
Published: March 2009

AbstractSix novel metabolites, nodulisporins D–F (1–3), (3S,4S,5R)‐2,4,6‐trimethyloct‐6‐ene‐3,5‐diol (4), 5‐hydroxy‐2‐hydroxymethyl‐4H‐chromen‐4‐one (5) and 3‐(2,3‐dihydroxyphenoxy)butanoic acid (6) were isolated together with seven known compounds (7–13) from the culture extract of the endophytic fungus Nodulisporium sp. The relative configurations of these new compounds were determined by means of spectroscopic data, including HREIMS, 1H NMR, 13C NMR and 2D NMR (HMQC, HMBC and NOESY). The novel compounds exhibit antifungal and antialgal activities and most of them were also antibacterial.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Cascade Annulation Reactions To Access the Structural Cores of Stereochemically Unusual Strychnos Alkaloids
Authors: Delgado Ricardo , Blakey Simon B. 
Published: March 2009

AbstractA new cascade annulation reaction has been developed to access the core structures of a novel family of strychnos alkaloids with a unique stereochemical arrangement. The new annulation cascade is facilitated by the development of a robust reaction sequence to access extremely sensitive N‐acyliminium ions. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Emergency preparation and green engineering: Augmenting the environmental knowledge and assessment tool
Authors: Whiteley Clinton E. , Boguski Terrie , Erickson Larry , Anthony Jennifer L. , Green Ryan 
Published: March 2009

AbstractAs our society continues to better prepare itself to address biological, radiological, chemical, and environmental emergencies, there is a need for better and more readily available emergency planning information for program managers and military or business personnel. Incorporating an online hazardous materials (HAZMATs) and emergency planning tool into the Environmental Knowledge and Assessment Tool (EKAT: www.ekat‐tool.com) would adequately fill that need. Although not yet fully functional, the project report that this manuscript is based on is available for viewing on the EKAT web site.The proposed online emergency preparation and green engineering (EPGE) tool would provide the user with information regarding links to local emergency response teams and resources, guides for developing emergency plans and reports, HAZMATs training information, case studies to illustrate HAZMAT situations, and the ability to judge the environmental greenness of chemicals. In this way, it will serve as a means of facilitating and educating individuals for best responses in an organized fashion.To address their environmental responsibilities, public and private organizations are adopting environmental management systems (EMS). The EPGE tool is used in conjunction with EMS to address sustainability. Currently, the creation of a comprehensive tool that identifies environmental, health, and safety concerns along with supplying relevant emergency data is applicable to any business or organization. It is designed to be used as a guide to characterize and solve the environmental issues that could affect any business. © 2009 American Institute of Chemical Engineers Environ Prog, 2009
Parameterizing a physically based shallow landslide model in a data poor region
Authors: Kuriakose Sekhar L. , van Beek L. P. H. , van Westen C. J. 
Published: March 2009

AbstractShallow landslides and consequent debris flows are an increasing concern in the Western Ghats of Kerala, India. Their increased frequency has been associated with deforestation and unfavourable land‐use practices in cultivated areas. In order to evaluate the influence of vegetation on shallow slope failures a physically based, dynamic and distributed hydrological model (STARWARS) coupled with a probabilistic slope stability model (PROBSTAB) was applied to the upper Tikovil River basin (55·6 km2). It was tuned with the limited evidence of groundwater conditions during the monsoon season of 2005 and validated against observed landslide activity in the hydrological year 2001–2002. Given the data poor conditions in the region some modifications to the original model were in order, including the estimation of parameters on the basis of generalized information from secondary sources, pedo‐transfer functions, empirical equations and satellite remote sensing data. Despite the poor input, the model captured the general temporal and spatial pattern of instability in the area. Sensitivity analysis proved root cohesion, soil depth and angle of internal friction as the most dominant parameters influencing slope stability. The results indicate the importance of root cohesion in maintaining stability and the critical role of the management of rubber plantations in this. Interception and evapotranspiration showed little influence on the development of failure conditions. The study also highlights the importance of high resolution digital terrain models for the accurate mechanistic prediction of shallow landslide initiation. Copyright © 2009 John Wiley & Sons, Ltd.
Composite Cathodes for Proton Conducting Electrolytes
Authors: Fabbri E. , Licoccia S. , Traversa E. , Wachsman E. D. 
Published: March 2009

AbstractDifferent composite materials made of mixed protonic/electronic conductors, SrCe0.9Yb0.1O3–δ (10YbSC) or BaCe0.9Yb0.1O3–δ (10YbBC), and a mixed oxygen‐ion/electronic conductor, La0.6Sr0.4Co0.2Fe0.8O3 (LSCF), were investigated for cathode application in intermediate temperature solid oxide fuel cells (IT‐SOFCs) using a high temperature proton conducting BaCe0.8Y0.2O3–δ electrolyte. Only the LSCF/10YbBC composite was found to be chemically stable. Area specific resistance (ASR) measurements were performed in wet air for LSCF/10YbBC cathodes, changing the weight ratio between the phases and the sintering procedure. The best performance was obtained for the composite cathode containing 50 wt.‐% of LSCF and 50 wt.‐% of 10YbBC, sintered at 1,100 °C. Electrochemical impedance spectroscopy (EIS) measurements of the tested cathodes showed two depressed semicircles in the middle and low frequency range, respectively. Performing ASR measurements at different p allowed us to attribute the two semicircles to charge transfer and oxygen diffusion processes, respectively. The microstructure of the LSCF/10YbBC(1:1) composite cathode was optimised changing the ratio of the particle sizes between the two phases. The lowest ASR values (0.14 Ω cm2 at 700 °C) were observed for the LSCF/10YbBC(1:1) composite cathode with different particle size (sub‐micrometer particles for LSCF and nanometer particles for 10YbBC). Fuel cell polarisation curves demonstrated superior performance of the LSCF/10YbBC (1:1) cathode with respect to Pt.
Altered frontal‐parietal functioning during verbal working memory in children and adolescents with heavy prenatal alcohol exposure
Authors: O'Hare Elizabeth D. , Lu Lisa H. , Houston Suzanne M. , Bookheimer Susan Y. , Mattson Sarah N. , O'Connor Mary J. , Sowell Elizabeth R. 
Published: March 2009

AbstractThis study evaluated the neural basis of verbal working memory (WM) function in a group of 20 children and adolescents with fetal alcohol spectrum disorders (FASDs) and 20 typically developing comparison participants using functional magnetic resonance imaging (fMRI). Both groups showed prominent activation in the frontal‐parietal‐cerebellar network known to be important for verbal WM. Despite equivalent behavioral performance between groups, alcohol‐exposed individuals showed increased activation relative to typically developing individuals in left dorsal frontal and left inferior parietal cortices, and bilateral posterior temporal regions during verbal WM. These effects remained even when group differences on IQ were statistically controlled. This pattern of increased activation coupled with equivalent behavioral performance between groups suggests that individuals with FASD recruit a more extensive network of brain regions during verbal WM relative to typically developing individuals. These findings may suggest that frontal‐parietal processing during verbal WM is less efficient in alcohol‐exposed individuals. Hum Brain Mapp 2009. © 2009 Wiley‐Liss, Inc.
Association between transforming growth factor β1 genetic polymorphism and response to chemoradiotherapy in head and neck squamous cell cancer
Authors: Lundberg Marie , Pajusto Mervi , Koskinen Walter J. , Mäkitie Antti A. , Aaltonen Leena‐Maija , Mattila Petri S. 
Published: March 2009

AbstractBackground.Transforming growth factor β (TGF‐β) is a pleiotropic cytokine that has diverse roles in cancer. Rate of production of the major isoform, TGF‐β1, is linked with rs1982073 single nucleotide polymorphism in TGFB1 gene signal sequence.Methods.Peripheral blood DNA of 175 head and neck squamous cell carcinoma patients were genotyped using real‐time PCR and fluorescent probes. The median follow‐up time was 2.9 years (range, 0.1–15.9 years). Survival was assessed using Cox regression.Results.Among the 38 patients who had received chemoradiotherapy without surgical resection the high‐producer TGFB1 genotypes CC and CT were associated with a better disease‐free and overall survival when compared with the low‐producer TT genotype (hazard ratios for interaction 3.42, 95% CI 1.12–10.5 and 3.09, 95% CI 0.96–10.0, respectively).Conclusion.Genetic polymorphism of the TGFB1 signal sequence is associated with the response to chemoradiotherapy. TGF‐β1 may sensitize cancer stem cells to chemoradiotherapy. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Bisphosphonate‐associated osteonecrosis of the jaws: The limits of a conservative approach
Authors: Tirelli Giancarlo , Biasotto Matteo , Chiandussi Silvia , Dore Franca , De Nardi Elena , Di Lenarda Roberto 
Published: March 2009

AbstractBackground.An increasing number of cases of osteonecrosis of the jaws (ONJ) in patients treated with bisphosphonates has been reported in the literature. ONJ significantly affects the patients' quality of life and its management is still extremely difficult.Methods.A woman with ONJ secondary to therapy with zoledronic acid came to our attention for recurrent oral infections and orocutaneous fistula unresponsive to antibiotic therapy combined with minor surgical debridements. The patient underwent major surgery to remove the fistula and clean the necrotic bone and soft tissues; a lobed skin platysma flap was used to close the defect. The treatment outcome was good.Conclusion.When ONJ fails to respond to antibiotic therapy and surgical debridements, a more invasive surgical approach may be necessary to guarantee a better quality of life for the patient. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Reverse facial artery–submental artery mandibular osteomuscular flap for the reconstruction of maxillary defects following the removal of benign tumors
Authors: Chen Wei‐Liang , Ye Jian‐Tao , Yang Zhao‐Hui , Huang Zhi‐Quan , Zhang Da‐Ming , Wang Ke 
Published: March 2009

AbstractBackground.Functional and aesthetic restoration in maxillary reconstruction remains a challenge. Although many free flap procedures have become popular in maxillary reconstruction, these microsurgical methods have certain limitations and risks. This study assessed the reliability of the reverse facial artery–submental artery mandibular osteomuscular flap for reconstructing maxillary defects.Methods.Eight maxillary defects following benign tumor ablation were repaired with reverse facial artery–submental artery mandibular osteomuscular flaps. The patients ranged in age from 16 to 33 years; 5 were male and 3 were female. Maxillary odontogenic myxoma was present in 3 cases, maxillary fibrous dysplasia and ameloblastoma in 2 cases each, and chondromyxoid fibroma in 1 case. The defects were classified as class 2a.Results.Primary reconstruction of the maxilla was carried out using a pedicled mandibular osteomuscular flap. No flap failures occurred. Dental reconstruction was successful in all patients. Proper aesthetics and complete functionality were obtained, and there were no donor‐site problems. The patients were followed for 12 to 24 months, with an average of 18.6 months, and no recurrence was encountered.Conclusion.The reverse facial artery–submental artery mandibular osteomuscular flap is safe, quick, and simple to elevate. The flap can be used reliably for reconstructing maxillary defects. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Increased mRNA expression of ADAMTS metalloproteinases in metastatic foci of head and neck cancer
Authors: Demircan Kadir , Gunduz Esra , Gunduz Mehmet , Beder Levent Bekir , Hirohata Satoshi , Nagatsuka Hitoshi , Cengiz Beyhan , Cilek Mehmet Zeynel , Yamanaka Noboru , Shimizu Kenji , Ninomiya Yoshifumi 
Published: March 2009

AbstractBackground.Although contribution of matrix metalloproteinases in cancer progression and dissemination is now well known, roles of recently discovered metalloproteinases, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS), in cancer development and progression remain mostly unknown.Methods.Here we examined the mRNA expression pattern of 6 members of ADAMTS aggrecanases (1, 4, 5, 8, 9, and 15) in primary head and neck cancer with and without metastasis by real‐time reverse transcriptase‐polymerase chain reaction.Results.Expression levels of ADAMTS mRNAs were lower in the majority of the primary tumors as compared with the controls. On the other hand, the expression levels of all of the ADAMTS mRNAs except ADAMTS4 were higher in the metastatic foci than in their corresponding primary tumors, which suggest that characteristics of the cancer cell population are different in the primary tumor and metastatic focus.Conclusion.Our findings suggest a metastasis model proposing accumulation of a subtype of cancer cells with high metastatic capacity within heterogenous primary tumor cell population.© 2009 Wiley Periodicals, Inc. Head Neck, 2009
Targeted radionuclide therapy of melanoma: Anti‐tumoural efficacy studies of a new 131I labelled potential agent
Authors: Bonnet‐Duquennoy Mathilde , Papon Janine , Mishellany Florence , Labarre Pierre , Guerquin‐Kern Jean‐Luc , Wu Ting‐Di , Gardette Maryline , Maublant Jean , Penault‐Llorca Frédérique , Miot‐Noirault Elisabeth , Cayre Anne , Madelmont Jean‐Claude , Chezal Jean‐Michel , Moins Nicole 
Published: March 2009

AbstractIn recent years, there has been dramatic worldwide increase in incidence of malignant melanoma. Although localised disease is often curable by surgical excision, metastatic melanoma is inherently resistant to most treatments. In this context, targeted radionuclide therapy could be an efficient alternative. After pharmacomodulation study, we selected a quinoxaline derivative molecule (ICF01012) for its high, specific and long‐lasting uptake in melanoma with rapid clearance from nontarget organs providing suitable dosimetry parameters for targeted radiotherapy. Aim of this study was to investigate, in vivo, efficacy of [131I]ICF01012 on nonmetastatic B16F0, metastatic B16Bl6 or human M4Beu melanoma tumours. First, colocalisation of ICF01012 with melanin by SIMS imaging was observed. Second, we showed that treatment drastically inhibited growth of B16F0, B16Bl6 and M4beu tumours whereas [131I]NaI or unlabelled ICF01012 treatment was without significant effect. Histological analysis and measure of PCNA proliferation marker expression showed that residual B16 tumour cellsexhibit a significant loss of aggressiveness after treatment. This effect is associated with a lengthening of the treated‐mice survival time. Moreover, with B16Bl6 model, 55% of the untreated mice had lung metastases whereas no metastasis was counted on treated group. Our data demonstrated a strong anti‐tumoural effect of [131I]ICF01012 for radionuclide therapy on murine and human in vivo pigmented melanoma models, whatever their dissemination profiles and their melanin content be. Further studies will attempt to optimise therapy protocol by increasing the balance between the anti‐tumoural effect and the safety on nontarget organs. © 2009 UICC