bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunolocalization of interleukin‐1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies
Authors: Grundtman Cecilia , Salomonsson Stina , Dorph Christina , Bruton Joseph , Andersson Ulf , Lundberg Ingrid E. 
Published: February 2007

AbstractObjectiveInterleukin‐1 (IL‐1) acts via its receptors to induce gene expression that mediates protein synthesis involved in inflammation. Increased expression of IL‐1α and IL‐1β in muscle tissue from patients with polymyositis and dermatomyositis has been demonstrated. It is not known whether the reciprocal IL‐1 receptors are expressed in human muscle tissue. The purpose of this study was to investigate the expression of IL‐1 receptors and their ligands in muscle tissue from patients with myositis and from healthy controls.MethodsMuscle biopsy tissues from 10 patients with polymyositis or dermatomyositis and 7 healthy control subjects were investigated by immunohistochemistry using antibodies against IL‐1 receptor type I (IL‐1RI), IL‐1RII, IL‐1α, IL‐1β, and IL‐1 receptor antagonist (IL‐1Ra). Quantification was performed by computerized image analysis, and localization of expression was determined by double staining using immunofluorescence and confocal microscopy.ResultsIn tissue samples from the patients, IL‐1RI and IL‐1RII were expressed in muscle fibers, inflammatory cells, and endothelial cells. Expression in muscle fibers was localized to the sarcolemma and nuclei. IL‐1α was expressed in endothelial cells and inflammatory cells, whereas IL‐1β and IL‐1Ra were expressed only in inflammatory cells. Expression of the two IL‐1 receptors and their ligands was significantly higher in patients than in controls. IL‐1 receptor expression on muscle fibers was most pronounced in the vicinity of cells expressing IL‐1α and IL‐1β.ConclusionThe increased expression of IL‐1 receptor and the colocalization with reciprocal ligands in patients with myositis but not in healthy controls support the hypothesis of a crucial role of IL‐1 in the pathogenesis of polymyositis and dermatomyositis.
A novel DNA vaccine targeting macrophage migration inhibitory factor protects joints from inflammation and destruction in murine models of arthritis
Authors: Onodera Shin , Ohshima Shigeki , Tohyama Harukazu , Yasuda Kazunori , Nishihira Jun , Iwakura Yoichiro , Matsuda Ikkei , Minami Akio , Koyama Yoshikazu 
Published: February 2007

AbstractObjectivePrevious studies have demonstrated that neutralization of macrophage migration inhibitory factor (MIF) by anti‐MIF antibodies decreases joint inflammation and destruction in a type II collagen–induced arthritis model in mice. The aim of this study was to develop and describe a simple and effective method of active immunization that induces anti‐MIF autoantibodies, which may neutralize MIF bioactivity.MethodsWe developed a MIF DNA vaccine by introducing oligonucleotides encoding a tetanus toxoid (TTX) Th cell epitope into the complementary DNA sequence of murine MIF. Mice were injected with this construct in conjunction with electroporation. The ability of this immunization to inhibit the development of collagen antibody–induced arthritis (CAIA) in BALB/c mice and spontaneous autoimmune arthritis in interleukin‐1 receptor antagonist (IL‐1Ra)–deficient mice was then evaluated.ResultsMice that received the MIF/TTX DNA vaccine developed high titers of autoantibodies that reacted to native MIF. Compared with unvaccinated mice, vaccinated mice also produced less serum tumor necrosis factor α after receiving an intravenous injection of lipopolysaccharide. In addition, vaccination with MIF/TTX DNA resulted in significant amelioration of both CAIA in BALB/c mice and symptoms of autoimmune arthritis in IL‐1Ra–knockout mice.ConclusionThese results suggest that MIF/TTX DNA vaccination may be useful for ameliorating the symptoms of rheumatoid arthritis.
Effect of recreational physical activities on the development of knee osteoarthritis in older adults of different weights: The Framingham Study
Authors: Felson David T. , Niu Jingbo , Clancy Margaret , Sack Burton , Aliabadi Piran , Zhang Yuqing 
Published: February 2007

AbstractObjectiveTo evaluate the long‐term effect of recreational exercise on the development of knee osteoarthritis (OA) in a community‐based cohort of older adults, many of whom were overweight or obese.MethodsSubjects were asked about recreational activities including walking or jogging for exercise and working up a sweat, and were asked to compare their activity levels with others. Subjects were then asked about knee pain and weight‐bearing anteroposterior and lateral knee radiographs were obtained. Approximately 9 years later, subjects were reexamined for OA. Radiographs were read for OA features in both tibiofemoral and patellofemoral compartments and were scored for tibiofemoral joint space narrowing. To evaluate incident OA, we excluded knees with OA at baseline for all analyses and focused on 3 knee‐specific outcomes: incident radiographic OA, symptomatic OA, and tibiofemoral joint space loss. After adjusting for age, sex, body mass index (BMI), knee injury history, and correlation between knees, we evaluated the association of each recreational activity with OA development.ResultsA total of 1,279 subjects underwent both baseline and followup examinations (mean age at baseline 53.2 years). Neither recreational walking, jogging, frequent working up a sweat, nor high activity levels relative to peers were associated with a decrease or increase in risk of OA. Joint space loss was also unaffected by activity. Persons with BMI above the median (27.7 kg/m2 for men and 25.7 kg/m2 for women; mean BMI >30 kg/m2 for both) had no increases in risk of OA by different type of activity.ConclusionAmong middle‐aged and elderly persons without knee OA, many of whom were overweight, recreational exercise neither protects against nor increases risk of knee OA.
Evaluation and validation of the patient acceptable symptom state (PASS) in patients with ankylosing spondylitis
Authors: Maksymowych Walter P. , Richardson Rhonda , Mallon Catherine , van der Heijde Désirée , Boonen Annelies 
Published: February 2007

AbstractObjectiveThe Patient Acceptable Symptom State (PASS) constitutes an absolute level of patient well‐being and represents an ambitious target for disease management. We explored contributors to PASS, validated the PASS concept, and assessed thresholds of self‐reported outcomes below which patients considered themselves in PASS.MethodsPatients with ankylosing spondylitis completed a questionnaire that included self‐reported assessments of pain, fatigue, disease activity, function, patient global, quality of life (QOL), and whether they considered their current disease state satisfactory or not. Stepwise logistic regression was used to assess contributors to PASS. PASS was validated by analyzing proportions of patients reporting need for a rheumatologist and who were in current flare. PASS thresholds for self‐reported outcomes were estimated using an anchoring method based on the patient's opinion and targeting the 75th percentile of the cumulative distribution.ResultsPASS data were available for 291 patients, of whom 169 (58%) were in PASS. Significant contributors were age (Exp[B] 1.05; P = 0.003), patient global disease activity (Exp[B] 0.79; P = 0.008), and function (Bath Ankylosing Spondylitis Functional Index [BASFI]; Exp[B] 0.72; P < 0.001). PASS reflected need to consult the rheumatologist and current flare (71% and 73% correctly classified, respectively) and significantly contributed to QOL (B = −5.99; 95% confidence interval −7.16, −4.08). PASS thresholds were 5.0 for patient global disease activity, 5.0 for total back pain, 22.8 for fatigue, 4.8 for disease activity (Bath Ankylosing Spondylitis Disease Activity Index), and 4.0 for function (BASFI).ConclusionA majority of patients (58%) reported being in PASS. PASS thresholds for pain and function were unexpectedly high, possibly suggesting adaptation to the consequences of the disease.
A new risk model based on policy entrance process and its weak convergence properties
Authors: Li Zehui , Kong Xinbing 
Published: February 2007

AbstractIn this paper, we construct a new risk model based on the policy entrance process. The model is concerned with n kinds of independent policies, and each policy is allowed to claim more than once before it expires. As each kind of policy is issued according to a non‐homogeneous Poisson process, the long run behaviour of the new risk process is investigated. When the tail of the claim size distribution is regularly varying, the standardized risk process is proved to converge to a stable law. When each kind of policy is issued according to a homogeneous Poisson process, we also give a diffusion approximation of the new risk process. Copyright © 2007 John Wiley & Sons, Ltd.
The orbit of the visual binary ADS 8630 (γ Vir)
Authors: Scardia M. , Argyle R. W. , Prieur J.‐L. , Pansecchi L. , Basso S. , Law N. M. , Mackay C. D. 
Published: February 2007

AbstractWe present a new orbit for the visual binary ADS 8630 = γ Vir. Although it is one of the first visual double stars discovered, its orbital elements were still poorly known. Indeed the very high eccentricity of the orbit and the difficulty of observing the pair at periastron passage in 1836 has meant that it is only now that sufficient measures of the recent close approach in 2005 have allowed an orbital analysis which predicts the angular motion to an acceptable degree of accuracy.We present a series of 35 speckle measurements of ADS 8630 obtained with PISCO in Merate between 2004 and 2006. Those measures have been crucial for determining the new orbital elements since they cover an arc of 130 degrees in the apparent orbit and include the periastron passage of 2005. The masses of the individual F0V components of the binary are found to be 1.40 M⊙ with an accuracy of about 3%.We also investigate in detail the possibility of the presence of a third body in the system, that was proposed by other authors. The high‐angular resolution infra‐red image of γ Vir that we obtained in June 2006 with the LuckyCam instrument on the ESO NTT shows the absence of any companion as faint as a M0V star at a distance larger than 0.4″. Combined with the analysis of the residuals of our orbit, the values found for the masses of the individual components and the radial velocity measurements, this observation rules out the presence in the system of a third companion with a mass larger than 0.3 M⊙. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
New ferrocene containing peptide conjugates: Synthesis and effect on human leukemia (HL‐60) cells
Authors: Miklán Zsanett , Szabó Rita , Zsoldos‐Mády Virág , Reményi Judit , Bánóczi Zoltán , Hudecz Ferenc 
Published: February 2007

AbstractData reported in this article describe the synthesis of Arg‐rich oligopeptide conjugates of ferrocenecarboxylic acid on solid support with two different strategies and for the first time, the successful preparation of peptide conjugates of ferrocenylacrylic acid in solution. The antitumor effect of conjugates was analyzed by MTT assay in vitro. We demonstrated that ferrocenylacrylic acid possessing an enone (CHCHCO) moiety exhibited remarkable antiproliferative effect against human leukemia cells (HL‐60) in vitro, but its effect was not improved by conjugation with hexa‐ or octaarginines. However, we observed highly increased water‐solubility. In contrast, the results provide evidence that conjugation of ferrocenecarboxylic acid to Argn (n = 6, 8) improved not only its water‐solubility, but also antitumor effect on human leukemia cells in vitro. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 88: 108–114, 2007.This article was originally published online as an accepted preprint. The ‘Published Online’ date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Peptide insertion, positioning, and stabilization in a membrane: Insight from an all‐atom molecular dynamics simulation
Authors: Babakhani Arneh , Gorfe Alemayehu A. , Gullingsrud Justin , Kim Judy E. , Andrew McCammon J. 
Published: February 2007

AbstractPeptide insertion, positioning, and stabilization in a model membrane are probed via an all‐atom molecular dynamics (MD) simulation. One peptide (WL5) is simulated in each leaflet of a solvated dimyristoylglycero‐3‐phosphate (DMPC) membrane. Within the first 5 ns, the peptides spontaneously insert into the membrane and then stabilize during the remaining 70 ns of simulation time. In both leaflets, the peptides localize to the membrane interface, and this localization is attributed to the formation of peptide–lipid hydrogen bonds. We show that the single tryptophan residue in each peptide contributes significantly to these hydrogen bonds; specifically, the nitrogen heteroatom of the indole ring plays a critical role. The tilt angles of the indole rings relative to the membrane normal in the upper and lower leaflets are approximately 26° and 54°, respectively. The tilt angles of the entire peptide chain are 62° and 74°. The membrane induces conformations of the peptide that are characteristic of β‐sheets, and the peptide enhances the lipid ordering in the membrane. Finally, the diffusion rate of the peptides in the membrane plane is calculated (based on experimental peptide concentrations) to be approximately 6 Å2/ns, thus suggesting a 500 ns time scale for intermolecular interactions. © 2007 Wiley Periodicals, Inc. Biopolymers 85: 490–497, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Metabolic engineering of the baculovirus‐expression system via inverse “shotgun” genomic analysis and RNA interference (dsRNA) increases product yield and cell longevity
Authors: Kim Eun Jeong , Kramer Shannon F. , Hebert Colin G. , Valdes James J. , Bentley William E. 
Published: February 2007

AbstractRNA interference (RNAi) is as powerful tool for characterizing gene function in eukaryotic organisms and cultured cell lines. Its use in metabolic engineering has been limited and few reports have targeted protein expression systems to increase yield. In this work, we examine the use of in vitro synthesized double stranded RNA (dsRNA) in the baculovirus expression vector system (BEVS), using commercially relevant cultured cells (Spodoptera frugiperda, Sf‐9) and larvae (Trichoplusia ni) as hosts. First, we employed an inverse “shotgun” genomic analysis to “find” an array of 16 putative insect gene targets. We then synthesized dsRNA in vitro targeting these genes and investigated the effects of injected dsRNA on larval growth, development, and product yield. Growth and development was at times stunted and in several cases, the effects were lethal. However, dsRNA targeting an acidic juvenile hormone‐suppressible protein (AJHSP1), and translational elongation factor 2 (Ef‐2) resulted in significantly increased yield of model product, GFP. Next, we targeted known genes, v‐cath and apoptosis inducer, sf‐caspase 1, in cultured Sf‐9 cells. We confirm RNAi‐mediated sf‐caspase 1 suppression in Sf‐9 cells, but not in baculovirus‐infected cells, likely due to the overriding effects of inhibitor of apoptosis protein, p35. We also demonstrate suppression of v‐cath in infected cells, which leads to a ∼3‐fold increase in product yield. Overall, our results support the application of RNAi in metabolic engineering, specifically for enhancing protein productivity in the baculovirus expression vector system. Biotechnol. Bioeng. 2007;98: 645–654. © 2007 Wiley Periodicals, Inc.
Di(benzothiazol‐2‐yl)phosphanide as a Janus‐Head Ligand to Caesium
Authors: Stey Thomas , Pfeiffer Matthias , Henn Julian , Pandey Sushil K. , Stalke Dietmar 
Published: February 2007

AbstractStarting from tris(benzothiazol‐2‐yl)phosphane (1) an advanced Janus‐head ligand, di(benzothiazol‐2‐yl)phosphane (2), was synthesised and structurally characterised. The heteroaryl substituents of this ligand provide both hard and soft donor sites. Surprisingly, the phosphorus atom in 2 is divalent and the hydrogen atom is directly bonded to one ring nitrogen atom and hydrogen bonded to the second. Compound 2 decomposes in any common solvent other than diethyl ether and a new preparation to improve the yields of 2 is presented. A coordination polymer, [{Cs(bth)2P}8] (3) (bth=benzothiazol‐2‐yl), was obtained when the sec‐phosphane 2 was allowed to react with elemental caesium in a 1:1 ratio in diethyl ether at −78 °C. In 3 each anion is coordinated to four caesium cations and vice versa. The central phosphorus atom is coordinated to two metal atoms above and below the mean plane of the anion in positions in which the two lone pairs of a four‐electron donor are anticipated. Two additional cations μ‐bridge both ring nitrogen atoms. Hence both faces of the Janus‐head ligand are coordinated to the same number of metal cations but in a different way.
Entrapped Bonded Hydrogen in a Fullerene: the Five‐Atom Cluster Sc3CH in C80
Authors: Krause Matthias , Ziegs Frank , Popov Alexey A. , Dunsch Lothar 
Published: February 2007

AbstractThe synthesis and characterisation of the new endohedral cluster fullerene Sc3CH@C80 is reported. The encapsulation of the first hydrocarbon cluster inside a fullerene was achieved by the arc burning method in a reactive CH4 atmosphere. The extensive characterisation by mass spectrometry (MS), high‐ pressure liquid chromatography (HPLC), 45Sc NMR, electron spin resonance (ESR), UV/Vis‐NIR and Raman spectroscopy provided the experimental evidence for the caging of the five‐atom Sc3CH cluster inside the C80 cage isomer with icosahedral symmetry. The proposed new structure was confirmed by DFT calculations, which gave a closed shell and large energy gap structure. Thus a pyramidal Sc3CH cluster and the Ih‐C80 cage were shown to be the most stable configuration for Sc3CH@C80 whereas alternative structures give a smaller bonding energy as well as a smaller energy gap.
Growth of ZnO single crystals by an induced nucleation from a high temperature solution of the ZnO‐PbF2 system
Authors: Li Xinhua , Xu Jiayue , Jin Min , Wu Xianjun , Qian Guoxing , Li Xiaomin 
Published: February 2007

AbstractTo grow ZnO single crystals from a high temperature solution of the ZnO‐PbF2 system, a gas cooling system was assembled at the bottom of the crucible to induce nucleation in the initial growth stage. The growth experiments were carried out in a homemade vertical Bridgman furnace and Pt crucible of 28 mm in diameter was used. The furnace temperature was set to 1100°C and the flow rate of the oxygen gas was optimized as 3.0 l/min. ZnO crystal up to 5∼8mm in the thickness was obtained with the lowering rate of 0.3 mm/h. XRD patterns showed that the as‐grown crystal was pure ZnO Wurtzite phase. The impurity ions were analyzed by the glow discharge mass spectroscopy (GDMS) as 390.0 ppm and 40.0 ppm for Pb2+ and F‐, respectively. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Protein crystal growth in gels and stationary magnetic fields
Authors: Moreno A. , Quiroz‐García B. , Yokaichiya F. , Stojanoff V. , Rudolph P. 
Published: February 2007

AbstractThaumatin, lysozyme, and ferritin single crystals were grown in solutions and gels without and with surrounding strong stationary magnetic fields. The crystal size, number and alignment in dependence on the induction force were analysed. The crystal quality, like mosaicity, as function of the magnetic force is discussed by using synchrotron X‐ray diffraction analysis. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Optical and laser properties of Nd:LuVO4crystal
Authors: Liu F. Q. , Xia H. R. , Gao W. L. , Ran D. G. , Sun S. Q. , Ling Z. C. , Zhao P. , Zhang H. J. , Zhao S. R. , Wang J. Y. 
Published: February 2007

AbstractPolarized absorption spectra of Nd:LuVO4crystal were measured at room temperature. The optical parameters of π polarization and σ polarization were calculated by Judd‐Ofelt theory. Meanwhile, the phenomenological intensity parameters: Ω2, Ω4, and Ω6were obtained, then the parameters were used to calculate the luminescence parameters of Nd:LuVO4crystal, including oscillator strengths, fluorescence branching ratio, radiative lifetime and integrated emission cross‐sections. Experiments of a‐cut and c‐cut Nd:LuVO4lasers were also performed, and opt‐opt conversion efficiency and slope efficiency were 40.3%, 50.5% for a‐cut and 23.6%, 30.9% for c‐cut, respectively. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effect of indium intercalation on various properties of MoSe2 single crystals
Authors: Vora Aditya M. 
Published: February 2007

AbstractIndium intercalated MoSe2 single crystals i.e. InxMoSe2 (0 ≤ x ≤ 1) are grown by direct vapour transport technique. These crystals are structurally characterized by X‐ray diffraction, by determining their lattice parameters ‘a’ and ‘c’ and X‐ray density. The Hall effect and thermoelectric power measurements shows that InxMoSe2 (0 ≤ x ≤ 1) are p‐type in nature. The direct and indirect band gap measurements are also undertaken on these semiconducting materials. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Physics, Chemistry and Applications of the AC Diaphragm Discharge and Related Discharges in Electrolyte Solutions
Authors: Maximov A. I. 
Published: February 2007

AbstractThree types of the underwater discharges produced by means of AC voltage sources (500‐2000 V, 0.05‐1 A) were investigated. Dynamic volt‐ampere characteristics and radiation intensity of these discharges were measured. It was observed the optical afterglow time up to 0.1‐0.2 s. The information about their chemical action was got by stimulation of the oxidation of the organic and inorganic substances. It was found that the treatment of the cellulose and the flax by means of the underwater discharges results in the change of their molecular mass, lignin contain and surface concentration of the oxygen containing functional groups. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Prevalence and natural history of monoclonal and polyclonal B‐cell lymphocytosis in a residential adult population
Authors: Shim Youn K. , Vogt Robert F. , Middleton Dan , Abbasi Fatima , Slade Barbara , Lee Kyung Y. , Marti Gerald E. 
Published: February 2007

AbstractBackground:Monoclonal B‐cells can be detected in the peripheral blood of some adults without B‐cell malignancies, a condition recently termed monoclonal B‐cell lymphocytosis (MBL). The risk of individuals with MBL progressing to a B‐cell malignancy is unknown. Polyclonal B‐cell lymphocytosis (PCBL) has not been systematically studied in the general population.Methods:We obtained lymphocyte subset counts on 1,926 residential adults aged 40–76 years in a series of environmental health studies between 1991 and 1994. We then conducted two follow‐ups in 1997 and 2003 on consenting participants with B‐cell lymphocytosis, which included nine participants with MBL. To ascertain the clinical implications of MBL, we reviewed medical records and death certificates.Results:The overall prevalence of MBL was 0.57% (11/1,926): nine cases at baseline and two additional cases identified at follow‐up. Two (19%) MBL cases subsequently developed a B‐cell malignancy; MBL persisted in the remaining nine cases (81%). All PCBL cases where no clone emerged regressed to normal B‐cell counts over the follow‐up period. MBL was significantly more frequent in residents near a hazardous waste site than in the control populations (age‐adjusted OR 6.2; 95%CI 1.1–36.2).Conclusion:MBL confers an elevated risk for developing a B‐cell malignancy, although it occurs only in a minority of cases. PCBL is most often a transient state, but a monoclonal population can emerge and persist. Prospective studies are needed to distinguish stable from progressive forms of B‐cell lymphocytosis and to clarify the etiologic role of environmental exposures. Published 2007 Wiley‐Liss, Inc.