bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reversible optic neuropathy with OPA1 exon 5b mutation
Authors: Cornille Karen , Milea Dan , Amati‐Bonneau Patrizia , Procaccio Vincent , Zazoun Lydie , Guillet Virginie , El Achouri Ghizlane , Delettre Cécile , Gueguen Naïg , Loiseau Dominique , Muller Agnès , Ferré Marc , Chevrollier Arnaud , Wallace Douglas C. , Bonneau Dominique , Hamel Christian , Reynier Pascal , Lenaers Guy 
Published: March 2008

AbstractA new c.740G>A (R247H) mutation in OPA1 alternate spliced exon 5b was found in a patient presenting with bilateral optic neuropathy followed by partial, spontaneous visual recovery. R247H fibroblasts from the patient and his unaffected father presented unusual highly tubular mitochondrial network, significant increased susceptibility to apoptosis, oxidative phosphorylation uncoupling, and altered OPA1 protein profile, supporting the pathogenicity of this mutation. These results suggest that the clinical spectrum of the OPA1‐associated optic neuropathies may be larger than previously described, and that spontaneous recovery may occur in cases harboring an exon 5b mutation. Ann Neurol 2008
Tobacco and pregnancy: Overview of exposures and effects
Authors: Rogers John M. 
Published: March 2008

AbstractThis opening article will review the epidemiology of the effects of cigarette smoking and other forms of tobacco exposure on human development. Sources of exposure described include cigarettes and other forms of smoked tobacco, secondhand (environmental) tobacco smoke, several forms of smokeless tobacco, and nicotine from nicotine replacement therapy. Exposure is immense and worldwide, most of it due to smoking, but in some parts of the world and in some populations, smoking is exceeded by smokeless tobacco use. Nicotine and carbon monoxide exposure are of large concern, but cigarette smoke contains over 4000 chemical constituents and additives including known carcinogens, toxic heavy metals, and many chemicals untested for developmental toxicity. The impact of tobacco on human development will be reviewed. Fertility, conception, survival of the conceptus, most phases and aspects of development studied to date, as well as postnatal survival and health are adversely impacted by maternal tobacco use or exposure. Effects in surviving offspring are probably life‐long, and are still being elucidated. It is hoped that this review and those to follow in this issue will serve to keep a focus on the critical and continuing problem of tobacco use impacting human development. Brith Defects Research (Part C) 84: 1–15, 2008. Published 2008 Wiley‐Liss, Inc.
Bauen im Bestand – Zuordnung historischer Betonfestigkeiten
Authors: Fingerloos Frank 
Published: March 2008

AbstractDie Beurteilung der Standsicherheit ist beim Bauen im Bestand von wesentlicher Bedeutung. Dabei sind Informationen über die früher verwendeten Baustoffe für die Planung, Ausschreibung und Bauausführung unerlässlich. Auf Basis der historischen Stahlbetonbestimmungen in Deutschland werden die Betone in Bezug auf die Festigkeitsklassen zugeordnet. Ausgehend von den ursprünglich zulässigen Beanspruchungen wird ein Vorschlag für die Annahme charakteristischer Druckfestigkeiten auf Basis des neuen Normenkonzepts gemacht.
An indeterminate occlusion duration predicts procedural failure in the recanalization of coronary chronic total occlusions
Authors: Barlis Peter , Kaplan Sahin , Dimopoulos Konstantinos , Tanigawa Jun , Schultz Carl , Di Mario Carlo 
Published: March 2008

AbstractObjectives:To identify the impact of occlusion duration (OD) and, in particular, an indeterminate occlusion duration (IOD) on immediate angiographic success and long‐term clinical outcomes in patients undergoing chronic total occlusion (CTO) percutaneous coronary intervention (PCI).Methods:From May 2003 to October 2006, all patients having PCI for a CTO were consecutively registered. In addition to an assessment of procedural outcomes, patients were followed long‐term for occurrence of major adverse cardiac events (MACE).Results:202 consecutive patients were included. Of these, 123 (60.9%) had a known OD while in the remaining 79 (39.1%), OD was indeterminate. Overall technical success was 82.7% and was lower in the IOD group (70.9% vs. 90.2%, P = 0.0004). An IOD was a predictor of procedural failure (adjusted OR 4.51, 95% CI 1.7–11.5, P = 0.002). An IOD (HR 2.59, 95%CI 1.08–6.23, P = 0.032) and procedural success (HR 0.26, 95%CI 0.11–0.60, P = 0.002) were the only independent predictors of long‐term MACE. Patients with a failed PCI and an IOD had a 5‐fold increased risk of MACE compared to those with known OD (37.0% vs. 7.1%, log rank P = 0.0036).Conclusions:In addition to the traditional predictors of procedural success and clinical outcome, this study, for the first time uncovered the importance of OD and, in particular, of an IOD. Still, in patients who are successfully recanalized, a long or IOD bears the same low rate of MACE as the general CTO population while in patients with failed PCI, an IOD carries a considerable risk of adverse prognosis. © 2008 Wiley‐Liss, Inc.
Feasibility of primary clot extraction prior to percutaneous coronary intervention in acute myocardial infarction
Authors: Aqel Raed , Zoghbi Gilbert , Hage Fadi , Philips George , Perry Gilbert , Iskandrian Ami , Dell'Italia Louis 
Published: March 2008

AbstractObjectives: To test the feasibility, safety, and in‐hospital outcomes of utilizing the FilterWire EZ to extract clot prior to percutaneous coronary intervention (PCI) in patients presenting with acute myocardial infarction (MI). Background: PCI in patients with acute MI is associated with a higher incidence of distal embolization, no‐reflow, or slow flow partly due to the presence of clot burden. Methods: The authors describe the feasibility, safety, and outcomes of using a FilterWire EZ distal protection device as a clot extraction device in patients who presented with acute MI and documented clot on coronary angiography. Results: Fifteen consecutive male patients with a mean age of 54 ± 8 years presented with acute MI (60% ST elevation MI). MI involved left anterior descending artery (n = 4), circumflex artery (n = 3), and right coronary artery (n = 8). Clot extraction followed by PCI reduced the percent diameter stenosis from 94 ± 12 to 65 ± 11 (P < 0.001) and restored TIMI 3 flow in all patients without distal embolization. The angiographic, procedural, and clinical success rates were 100%. The mean left ventricular ejection fraction (LVEF) was 52 ± 8% (range 30–62%) with only three patients (15%) who had an LVEF <50% and five patients (33%) without apparent wall motion abnormalities on echocardiography. Conclusions: Clot extraction before PCI during acute MI in native coronaries is feasible, safe, and effective in restoring TIMI 3 flow without distal embolization. Whether this approach results in better outcomes and improved LV function compared with standard therapy alone requires further investigation. © 2008 Wiley‐Liss, Inc.
Balloon angioplasty with stenting to correct a functionally interrupted aorta: A case report with three‐year follow‐up
Authors: Musso Tamara M. , Slack Michael C. , Nowlen Todd T. 
Published: March 2008

AbstractA 16‐year‐old male presenting with upper extremity hypertension was found to have a severe form of discrete coarctation with complete luminal obliteration, causing a functional interruption of the thoracic aorta. Fluoroscopically guided perforation of the obstruction and creation of a neo‐aortic lumen was performed. This was followed by balloon angioplasty and stent placement, successfully relieving the coarctation. The procedural method, acute and late follow‐up results, and a discussion of the potential risks and benefits are presented. © 2008 Wiley‐Liss, Inc.
Postnatal changes in the Rexed lamination and markers of nociceptive afferents in the superficial dorsal horn of the rat
Authors: Lorenzo Louis‐Etienne , Ramien Michele , St. Louis Manon , De Koninck Yves , Ribeiro‐Da‐Silva Alfredo 
Published: March 2008

AbstractIn this study, we investigated postnatal changes in Rexed's laminae and distribution of nociceptive afferents in the dorsal horn of the rat lumbar spinal cord at postnatal days 0, 5, 10, 15, 20, and 60. Transverse sections of the L4–L5 segments were processed for triple labeling with isolectin B4 (IB4)‐binding as a marker of nonpeptidergic C‐fibers, calcitonin gene‐related peptide (CGRP) immunoreactivity to label peptidergic nociceptive afferents, and a fluorescent Nissl stain to visualize cells and lamination at different stages of postnatal development. The Nissl staining revealed that the thickness of lamina I (LI) and outer lamina II remained mostly unchanged from birth until adulthood. CGRP afferents terminated mostly in LI and the outer two‐thirds of lamina II, whereas the termination area of fibers binding IB4 was centered on the middle one‐third of lamina II at all ages studied. In absolute values, the overall width of the bands of intense CGRP and IB4 labeling increased with age but decreased as a percentage of the overall thickness of the dorsal horn with maturation. The overlap of CGRP termination area with that of IB4 afferents increased with age. The consequences of these findings are twofold. First, the size of the different laminae does not grow evenly across the dorsal horn. Second, CGRP and IB4 labeling cannot be considered per se to be reliable markers of lamination during development. These findings have implications for comparing data obtained in immature and mature tissues with respect to localization of structures in the dorsal horn. J. Comp. Neurol. 508:592–604, 2008. © 2008 Wiley‐Liss, Inc.
Nuclear ferritin‐mediated protection of corneal epithelial cells from oxidative damage to DNA
Authors: Cai Cindy , Ching Anna , Lagace Christopher , Linsenmayer Thomas 
Published: March 2008

AbstractWe previously obtained evidence that ferritin is a nuclear protein in embryonic avian corneal epithelial (CE) cells, and that the ferritin in this site protects DNA from UV‐induced damage. UV irradiation is known to produce reactive oxygen species (ROS) and ferritin is known to ameliorate further oxidative damage by sequestering free iron, thus decreasing the formation of hydroxyl radicals through the Fenton reaction. Here we present evidence that nuclear ferritin can similarly prevent damage by the ROS, H2O2. These results, when coupled with our previous ones showing that nuclear ferritin appears in the CE early in its development, raises the possibility that this ferritin may serve two protective roles. The initial one would be during embryonic development to protect the CE from ROS endogenously produced by the embryo itself (e.g., H2O2; the latter one would be post‐hatching to protect the CE from environmentally produced oxidative insults (e.g., from U.V. light). Developmental Dynamics 237:2676–2683, 2008. © 2008 Wiley‐Liss, Inc.
SPARC is expressed by macroglia and microglia in the developing and mature nervous system
Authors: Vincent Adele J. , Lau Patricia W. , Roskams A. Jane 
Published: March 2008

AbstractSPARC (secreted protein, acidic and rich in cysteine) is a matricellular protein that is highly expressed during development, tissue remodeling, and repair. SPARC produced by olfactory ensheathing cells (OECs) can promote axon sprouting in vitro and in vivo. Here, we show that in the developing nervous system of the mouse, SPARC is expressed by radial glia, blood vessels, and other pial‐derived structures during embryogenesis and postnatal development. The rostral migratory stream contains SPARC that becomes progressively restricted to the SVZ in adulthood. In the adult CNS, SPARC is enriched in specialized radial glial derivatives (Müller and Bergmann glia), microglia, and brainstem astrocytes. The peripheral glia, Schwann cells, and OECs express SPARC throughout development and in maturity, although it appears to be down‐regulated with maturation. These data suggest that SPARC may be expressed by glia in a spatiotemporal manner consistent with a role in cell migration, neurogenesis, synaptic plasticity, and angiogenesis. Developmental Dynamics 237:1449‐1462, 2008. © 2008 Wiley‐Liss, Inc.
Defective positive selection results in T cell lymphopenia and increased autoimmune diabetes in ADAP‐deficient BDC2.5‐C57BL/6 mice
Authors: Zou Liangxing , Mendez Felipe , Martin‐Orozco Natalia , Peterson Erik J. 
Published: March 2008

AbstractAdhesion and degranulation promoting adapter protein (ADAP), a positive regulator of T cell receptor (TCR) signaling, is required for thymocyte development and T cell homeostasis. To investigate the role of ADAP in a T cell‐driven autoimmune response, we generated ADAP‐deficient, BDC2.5 TCR transgenic, diabetes‐prone (C57BL/6) mice (BDC/B6). We observed a striking enhancement of diabetes incidence in ADAP‐deficient mice, both in animals homozygous for I‐Ag7, and in mice carrying one I‐Ab allele (BDC/B6g7/b). Increased disease correlates with significantly reduced numbers of pathological CD4+ T cells in the mice. Consistent with a state of functional lymphopenia in ADAP‐deficient BDC/B6g7/b mice, T cells display increased homeostatic proliferation. Transfer of syngeneic lymphocytes or T cells both blocks ADAP‐dependent diabetes and relieves exaggerated homeostatic T cell proliferation observed in ADAP‐deficient mice. Marked attenuation in cellularity of the CD4+ single‐positive thymocyte compartment in ADAP‐deficient BDC/B6g7/b animals suggests a mechanism for induction of the lymphopenia. We conclude that inefficient positive selection in ADAP deficiency results in lymphopenia that leads to enhanced autoimmune diabetes in the BDC/B6g7/b model. Our findings support the notion that ineffective thymic T cell output can be a powerful causative factor in lymphopenia‐driven autoimmune diabetes.
CXCR3 determines strain susceptibility to murine cerebral malaria by mediating T lymphocyte migration toward IFN‐γ‐induced chemokines
Authors: Van den Steen Philippe E. , Deroost Katrien , Aelst Ilse Van , Geurts Nathalie , Martens Erik , Struyf Sofie , Nie Catherine Q. , Hansen Diana S. , Matthys Patrick , Damme Jo Van , Opdenakker Ghislain 
Published: March 2008

AbstractCerebral malaria (CM) results from the binding of infected erythrocytes and leukocytes to brain endothelia. The precise mechanisms underlying lymphocyte recruitment and activation in CM remain unclear. Therefore, the expression of various chemokines was quantified in brains of mice infected with Plasmodium berghei ANKA (PbA). Several chemokines attracting monocytes and activated T‐lymphocytes were expressed at high levels. Their expression was almost completely abrogated in IFN‐γ ligand and receptor KO mice, indicating that IFN‐γ is an essential chemokine inducer in vivo. Surprisingly, the expression levels of chemokines, IFN‐γ and also adhesion molecules in the brain were not lower in CM‐resistant Balb/c and DBA/2 mice compared to CM‐sensitive C57BL/6 and DBA/1 mice, although T lymphocyte sequestration in the brain was significantly less in CM‐resistant than in CM‐sensitive mice. This difference correlated with a higher up‐regulation of the CXC chemokine receptor (CXCR)‐3 on splenic T cells and a higher chemotactic response to IFN‐γ‐inducible protein‐10 (IP‐10) in C57BL/6 compared to Balb/c mice. In conclusion, parasite‐induced IFN‐γ in the brain results in high local expression levels of specific chemokines for monocytes and lymphocytes. The strain‐dependent susceptibility to develop CM is more related to the expression of CXCR3 in circulating leukocytes than to the chemokine expression levels in the brain.
Topochemical Synthesis of Micron‐Platelet (Na0.5K0.5)NbO3 Particles
Authors: Li Lihong , Chen Jun , Deng Jinxia , Yu Ranbo , Qiao Lijie , Liu Guirong , Xing Xianran 
Published: March 2008

AbstractMicron‐scale platelet (Na, K)NbO3 particles were synthesized from the platelet precursor K4Nb6O17 in a KCl medium using a topochemical method . The salt took part in the reaction and affected the composition of the products. Stoichiometric (Na0.5K0.5)NbO3 could be accurately synthesized by controlling the amount of the reactant Na2CO3. X‐ray diffraction analysis revealed that the crystallographic {010} plane of K4Nb6O17 was converted into the pseudo‐cubic {001} plane of (Na0.5K0.5)NbO3. The polycrystalline Na0.5K0.5NbO3 particles exhibited a plate‐like shape with a high aspect ratio, and were suitable for preparing textured ceramics by the template grain growth process. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
A Phenanthroline Heteroleptic Ruthenium Complex and Its Application to Dye‐Sensitised Solar Cells
Authors: Reynal Anna , Forneli Amparo , Martinez‐Ferrero Eugenia , Sanchez‐Diaz Antonio , Vidal‐Ferran Anton , Palomares Emilio 
Published: March 2008

AbstractWe report here the synthesis and characterization of a new heteroleptic ruthenium(II) complex and its applications as efficient light‐harvesting sensitizer in functional dye‐sensitized solar cells. The relation between the interfacial charge‐transfer processes that govern the device performance and the cell efficiency under illumination are also discussed. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Facile Entry to 4,5,6,7‐Tetrahydro[1,2,3]triazolo[1,5‐a]pyrazin‐6‐ones from Amines and Amino Acids
Authors: Sai Sudhir V. , Nasir Baig R. B. , Chandrasekaran Srinivasan 
Published: March 2008

AbstractA practical and high‐yielding regioselective synthesis ofseveral enantiopure 4,5,6,7‐tetrahydro[1,2,3]triazolo[1,5‐a]pyrazin‐6‐ones is described starting from primary amines and α‐amino acid derivatives in a three‐step reaction sequence by employing a constrained intramolecular “click” reaction as the key step. The method obviates chromatographic purification of products.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Analysis of phenolic compounds in extra virgin olive oil by using reversed‐phase capillary electrochromatography
Authors: Aturki Zeineb , Fanali Salvatore , D'Orazio Giovanni , Rocco Anna , Rosati Chiara 
Published: March 2008

AbstractIn this work, the simultaneous separation of ten phenolic compounds (protocatechuic, p‐coumaric, o‐coumaric, vanillic, ferulic, caffeic, syringic acids, hydroxytyrosol, tyrosol and oleuropein) in extra virgin olive oils (EVOOs) by isocratic RP CEC is proposed. A CEC method was optimized in order to completely resolve all the analyzed compounds by studying several experimental parameters. The influence of the stationary phase type (C18 and C8 modified silica gel), buffer concentration and pH as well as the organic modifier content of the mobile phase on retention factors, selectivity and efficiency were evaluated in details. A capillary column packed with Cogent® bidentate C18 particles for 23 cm and a mobile phase composed by 100 mM ammonium formate buffer pH 3/H2O/ACN (5:65:30 v/v/v) allowed the baseline resolution of the compounds under study in less than 35 min setting the applied voltage and temperature at 22 kV and 20°C, respectively. A study, evaluating the intra‐ and interday precision as well as LOD and LOQ and method linearity was developed in accordance with the analytical procedures for method validation. LODs were in the range of 0.015–2.5 μg/mL, while calibration curves showed a good linearity (r2 >0.997). The CEC method was applied to the separation and determination of these compounds in EVOO samples after a suitable liquid–liquid extraction procedure. The mean recovery values of the studied compounds ranged between 87 and 99%.
Two‐dimensional strong cation exchange/porous layer open tubular/mass spectrometry for ultratrace proteomic analysis using a 10 μm id poly(styrene‐ divinylbenzene) porous layer open tubular column with an on‐line triphasic trapping column
Authors: Luo Quanzhou , Gu Ye , Wu Shiaw‐Lin , Rejtar Tomas , Karger Barry L. 
Published: March 2008

AbstractThis study expands the capabilities for ultratrace proteomic analysis of our previous work by incorporating on‐line sample desalting using a triphasic (RP/strong cation exchange (SCX)/micro‐SPE) trapping column connected to a 3.2 m×10 μm id poly(styrene‐divinylbenzene) (PS‐DVB) porous layer open tubular (PLOT) column. To minimize extra sample handling steps, C18 RP packing was incorporated in the capillary tubing upstream of the SCX column for the on‐line desalting. For the micro‐SPE column, a 50 μm id PS‐DVB monolithic column was positioned downstream of the SCX column. High‐performance separation was achieved on the PLOT column at a mobile phase flow rate of 20 nL/min. The sensitivity and high resolution capability of the new multidimensional platform was evaluated using an in‐gel tryptic digested sample of a cervical cancer (SiHa) cell line. For the injected amount of 1200 cells (∼500 ng), over 2700 peptides covering greater than 850 unique proteins were identified from the triphasic SCX/PLOT/MS analysis of a single SDS gel section (>40 kDa). The 2‐D LC/MS platform demonstrated good separation performance, such that more than 85% of the identified peptides were detected from only one salt fraction. In a triplicate analysis of the above >40 kDa gel section, 4497 peptides and 1209 unique proteins were identified when applying stringent filtering criteria, with a false‐positive rate of 2.4%. When all three SDS‐PAGE gel sections of the lysed SiHa cells were analyzed, 5047 peptides and 1857 unique proteins (false‐positive rate 1.8%), including cancer‐related proteins such as MAP kinases, were identified.
Use of quasi‐isoelectric buffers as anolyte and catholyte to improve capillary isoelectric focusing performances
Authors: Poitevin Martine , Peltre Gabriel , Descroix Stephanie 
Published: March 2008

AbstractThe use of quasi‐isoelectric anolytes and catholytes has been investigated to improve CIEF performances. Narrow pH cuts of carrier ampholytes (NC) have been compared to more conventional couples of anolytes/catholytes (phosphoric acid/sodium hydroxide and glutamic acid/lysine). First, a CIEF setup that consists in a bare silica capillary and 70:30 water/glycerol separation medium has been used. The experiments have shown that when using NC instead of more classical anolytes and catholytes, an increase in the protein detection time was observed and the resolutions obtained for neutral and acidic proteins were doubled. Moreover, according to the NC fraction used, the resolution was modified. In order to investigate further the mechanisms involved, a second setup using a capillary coated with hydroxypropylcellulose was used. With this setup no difference has been observed when changing anolyte and catholyte nature. A simple methodology has then been developed to evaluate EOF during focusing and mobilization steps of CIEF experiments. It highlighted the crucial role played by EOF when using a bare silica capillary. EOF indeed decreased by 33% during mobilization step when using NC instead of classical anolytes and catholytes.