bluuebunny/crossref_metadata_2025_split huggingface dataset:
Powdery mildew-induced changes in phyllosphere microbial community dynamics of cucumber
Authors: Yue Cong , Du Changxia , Wang Xiaodan , Tan Yinqing , Liu Xingchen , Fan Huaifu 
Published: April 2024

Abstract
As an important habitat for microorganisms, the phyllosphere has a great impact on plant growth and health, and changes in phyllosphere microorganisms are closely related to the occurrence of leaf diseases. However, there remains a limited understanding regarding alterations to the microbial community in the phyllosphere resulting from pathogen infections. Here, we analyzed and compared the differences in phyllosphere microorganisms of powdery mildew cucumber from three disease severity levels (0% &lt; L1 &lt; 30%, 30% ≤ L2 &lt; 50%, L3 ≥ 50%, the number represents the lesion coverage rate of powdery mildew on leaves). There were significant differences in α diversity and community structure of phyllosphere communities under different disease levels. Disease severity altered the community structure of phyllosphere microorganisms, Rosenbergiella, Rickettsia, and Cladosporium accounted for the largest proportion in the L1 disease grade, while Bacillus, Pantoea, Kocuria, and Podosphaera had the highest relative abundance in the L3 disease grade. The co-occurrence network analysis of the phyllosphere microbial community indicated that the phyllosphere bacterial community was most affected by the severity of disease. Our results suggested that with the development of cucumber powdery mildew, the symbiotic relationship between species was broken, and the entire bacterial community tended to compete.
Case reports on uniparental disomy of chromosomes 6 and 3 in paternity testing
Authors: Ren He , Liu Zhiyong , Chen Chong , Shi Yan , Zhang Jiarong , Chen Ying , Jia Li , Liu Yacheng , Yan Jiangwei 
Published: April 2024

Abstract
In paternity testing, when there are Mendelian errors in the alleles between the child and the parents, a slippage mutation, or silent allele may not fully explain the phenomenon. Sometimes, it is attributed to chromosomal abnormalities, such as uniparental disomy (UPD). Here, we present the investigation of two cases of suspected UPD in paternity testing based on short tandem repeat (STR) detection (capillary electrophoresis platform). Case 1 involves a trio, where all genotypes detected on chromosome 6 in the child are homozygous and found in the father. Case 2 is a duo (mother and child), where all genotypes on chromosome 3 in the child are homozygous and not always found in the mother. At the same time, Mendelian error alleles were also observed at specific loci in these two chromosomes. Furthermore, we used the MGIEasy Signature Identification Library Prep Kit for sequencing on the massively parallel sequencing platform, which included common autosomal, X and Y chromosomes, and mitochondrial genetic markers used in forensic practice. The results showed that the genotypes of shared STRs on the two platforms were consistent, and STRs and single nucleotide polymorphisms (SNPs) on these two chromosomes were homozygous. All other genetic markers followed the laws of inheritance. A comprehensive analysis supported the parent–child relationship between the child and the alleged parent, and the observed genetic anomalies can be attributed to UPD. UPD occurrences are rare, and ignoring its presence can lead to erroneous exclusions in paternity testing, particularly when multiple loci on a chromosome exhibit homozygosity.
Inequalities in Healthcare Access, Experience and Outcomes in Adults With Inflammatory Bowel Disease: A Scoping Review
Authors: Hawkins Rachel L , Zia Maryam , Hind Daniel , Lobo Alan J 
Published: April 2024

Abstract

Background
Inflammatory bowel diseases (IBDs) are incurable diseases that require lifelong access to health services. Accumulating evidence of inequalities in health care access, experience, and outcomes for individuals with IBD is apparent. This review aimed to describe the inequalities in healthcare access, experiences, and outcomes of care for adults with IBD, to identify research gaps, and to identify future research priorities in this area.

Methods
A scoping review was conducted to retrieve quantitative, qualitative, and mixed methods evidence from 3 databases (EMBASE, Medline, and CINAHL) published between January 1, 2000, and September 27, 2023.

Results
Fifty-one studies met the criteria for inclusion. The majority (42 of 51) focused on IBD health outcomes, followed by healthcare access (24 of 51). Significantly fewer investigated patient experiences of IBD healthcare (8 of 51). Most available studies reported on race/ethnic disparities of healthcare (33 of 51), followed by inequalities driven by socioeconomic differences (12 of 51), rurality (7 of 51), gender and sex (3 of 51), age (2 of 51), culture (2 of 51), literacy (1 of 51), and sexuality (1 of 51). Inflammatory bowel disease patients from Black, Asian, and Hispanic ethnic groups had significantly poorer health outcomes. A lack of research was found in the sexual and gender minority community (1 of 51). No research was found to investigate inequalities in IBD patients with learning disabilities or autism.

Conclusions
Further research, particularly utilizing qualitative methods, is needed to understand health experiences of underserved patient populations with IBD. Cultural humility in IBD care is required to better serve individuals with IBD of Black and Asian race/ethnicity. The lack of research amongst sexual and gender minority groups with IBD, and with learning disabilities, poses a risk of creating inequalities within inequalities.
Exoskeletal Trade-off between Claws and Carapace in Deep-sea Hydrothermal Vent Decapod Crustaceans
Authors: Cho Boongho , Seo Hyein , Hong Junyoung , Jang Sook-Jin , Kim Taewon 
Published: April 2024

Synopsis
Limitations on energetic resources create evolutionary trade-offs, prompting us to investigate if investment in claw strength remains consistent across crustaceans living in diverse habitats. Decapod crustaceans living in deep-sea hydrothermal vents are ideal for this study due to their extreme environment. In this study, we investigated whether decapods (blind crab Austinograea sp. and the squat lobster Munidopsis lauensis) living in deep-sea hydrothermal vents prioritize investing in strong claws compared to the carapace, like coastal decapods. We analyzed exoskeleton morphology, mechanical properties, structures, and elemental composition in both the carapace and claws of four Decapoda species (two each from Brachyura and Anomura infraorders) in vent and coastal habitats. Coastal decapods had ∼4–9 times more teeth on their claw cutting edge than the vent species. Further, only the coastal species exhibited higher firmness in their claws than in their carapaces. Each infraorder controlled exoskeletal hardness differently: Brachyura changed the stacking height of the Bouligand structure, while Anomura regulated magnesium content in the exoskeleton. The vent decapods may prioritize strengthening their carapace over developing robust claws, allocating resources to adapt to the harsh conditions of deep-sea hydrothermal vents. This choice might enhance their survival in the extreme environment, where carapace strength is crucial for protecting internal organs from environmental factors, rather than relying on the powerful claws seen in coastal decapods for a competitive advantage.
Alternative venous access sites for dual-lumen extracorporeal membrane oxygenation cannulation
Authors: Schoeberl Armin-Kai , Staudacher Dawid , Kawashima Mitsuaki , Fischer Courtney , Cypel Marcelo , Buchtele Nina , Staudinger Thomas , Aigner Clemens , Hoetzenecker Konrad , Schweiger Thomas 
Published: April 2024

Abstract

OBJECTIVES
Dual-lumen cannulas for veno-venous (VV) extracorporeal membrane oxygenation (ECMO) support are typically inserted in the right internal jugular vein (RIJV); however, some scenarios can make this venous route inaccessible. This multicentre case series aims to evaluate if single-site cannulation using an alternative venous access is safe and feasible in patients with an inaccessible RIJV.

METHODS
We performed a multi-institutional retrospective analysis including high-volume ECMO centres with substantial experience in dual-lumen cannulation (DLC) (defined as &gt;10 DLC per year). Three centres [Freiburg (Germany), Toronto (Canada) and Vienna (Austria)] agreed to share their data, including baseline characteristics, technical ECMO and cannulation data as well as complications related to ECMO cannulation and outcome.

RESULTS
A total of 20 patients received alternative DLC for respiratory failure. Cannula insertion sites included the left internal jugular vein (n = 5), the right (n = 7) or left (n = 3) subclavian vein and the right (n = 4) or left (n = 1) femoral vein. The median cannula size was 26 (19–28) French. The median initial target ECMO flow was 2.9 (1.8–3.1) l/min and corresponded with used cannula size and estimated cardiac output. No procedural complications were reported during cannulation and median ECMO runtime was 15 (9–22) days. Ten patients were successfully bridged to lung transplantation (n = 5) or lung recovery (n = 5). Ten patients died during or after ECMO support.

CONCLUSIONS
Alternative venous access sites for single-site dual-lumen catheters are a safe and feasible option to provide veno-venous ECMO support to patients with inaccessible RIJV.
Theoretical performance assessment of a multi-effect distillation system integrated with thermal vapour compression unit running on solar energy
Authors: Shahzamanian B , Varga S , Soares J , Palmero-Marrero AI , Oliveira AC 
Published: April 2024

Abstract
Mathematical models can be successfully applied to predict the multi-effect distillation system integrated with thermal vapour compression unit (MED-TVC) performance under different operating conditions. In this work, a one-dimensional (1-D) mathematical model was developed to perform the theoretical analysis of a small-scale parallel feed MED-TVC desalination system driven by solar heat. A parametric study was conducted allowing the ejector motive steam temperature, first effect temperature, between effects temperature difference and ejector compression ratio to vary within an expectable range.
The results showed that higher motive steam temperatures, had a positive impact on the water yield up to an optimal point. Any additional increase of driving steam temperature reduced water yield. Gain output ratio ($GOR$) was maximum at the highest considered motive temperature. It was found that the calculated specific heat transfer area does not depend on the motive temperature variation. Water yield, $GOR$ and specific heat transfer area decrease with the ejector compression ratio. The temperature difference between the evaporator effects had no effect on the water yield and $GOR$. It only decreases the specific heat transfer area. First effect temperature should be as low as possible with respect to operational constraints. Based on the parametric study, the optimal design operating conditions, corresponding to the maximum water yield is identified for a small-scale three-effect desalination system. The unit can produce up to 204 l/h drinking water with a gain output ratio of 8.6 and specific heat transfer area of 575 m2/kg/s using about 22 kW of solar heat.
Outpatient Antipsychotic Use and Severe COVID-19: Avoiding the Impact of Age in a Real-World Data Study
Authors: Pintos-Rodríguez Samuel , Visos-Varela Irene , Rodríguez-Fernández Almudena , Zapata-Cachafeiro Maruxa , Piñeiro-Lamas María , Herdeiro María Teresa , García-Álvarez Rosa María , Figueiras Adolfo , Salgado-Barreira Ángel 
Published: April 2024

Abstract

Background
The association between use of antipsychotics and COVID-19 outcomes is inconsistent, which may be linked to use of these drugs in age-related diseases. Furthermore, there is little evidence regarding their effect in the nongeriatric population. We aim to assess the association between antipsychotic use and risk of disease progression and hospitalization due to COVID-19 among the general population, stratifying by age.

Methods
We conducted a population-based, multiple case-control study to assess risk of hospitalization, with cases being patients with a PCR(+) test who required hospitalization and controls being individuals without a PCR(+) test; and risk of progression to hospitalization, with cases being the same as those used in the hospitalization substudy and controls being nonhospitalized PCR(+) patients. We calculated adjusted odds-ratios (aOR) and 95% confidence intervals (CI), both overall and stratified by age.

Results
Antipsychotic treatment in patients younger than 65 years was not associated with a higher risk of hospitalization due to COVID-19 (aOR 0.94 [95%CI = 0.69–1.27]) and disease progression among PCR(+) patients (aOR 0.96 [95%CI = 0.70–1.33]). For patients aged 65 years or older, however, there was a significant, increased risk of hospitalization (aOR 1.58 [95% CI = 1.38–1.80]) and disease progression (aOR 1.31 [95% CI = 1.12–1.55]).

Conclusions
The results of our large-scale real-world data study suggest that antipsychotic use is not associated with a greater risk of hospitalization due to COVID-19 and progression to hospitalization among patients younger than 65 years. The effect found in the group aged 65 years or older might be associated with off-label use of antipsychotics.
Unveiling the hidden diversity and functional role of Chloroflexota in full-scale wastewater treatment plants through genome-centric analyses
Authors: Bovio-Winkler Patricia , Cabezas Angela , Etchebehere Claudia 
Published: April 2024

AbstractThe phylum Chloroflexota has been found to exhibit high abundance in the microbial communities from wastewater treatment plants (WWTPs) in both aerobic and anaerobic systems. However, its metabolic role has not been fully explored due to the lack of cultured isolates. To address this gap, we use publicly available metagenome datasets from both activated sludge (AS) and methanogenic (MET) full-scale wastewater treatment reactors to assembled genomes. Using this strategy, 264 dereplicated, medium- and high-quality metagenome-assembled genomes (MAGs) classified within Chloroflexota were obtained. Taxonomic classification revealed that AS and MET reactors harbored distinct Chloroflexota families. Nonetheless, the majority of the annotated MAGs (166 MAGs with &gt;85% completeness and &lt; 5% contamination) shared most of the metabolic potential features, including the ability to degrade simple sugars and complex polysaccharides, fatty acids and amino acids, as well as perform fermentation of different products. While Chloroflexota MAGs from MET reactors showed the potential for strict fermentation, MAGs from AS harbored the potential for facultatively aerobic metabolism. Metabolic reconstruction of Chloroflexota members from AS unveiled their versatile metabolism and suggested a primary role in hydrolysis, carbon removal and involvement in nitrogen cycling, thus establishing them as fundamental components of the ecosystem. Microbial reference genomes are essential resources for understanding the potential functional role of uncultured organisms in WWTPs. Our study provides a comprehensive genome catalog of Chloroflexota for future analyses aimed at elucidating their role in these ecosystems.
Conducting Care-full Research: Collaborative Research amidst Corona, a Coup, and Other Crises
Authors: Berents Helen , Mollica Caitlin , Odgers-Jewell Casey , Payne Hayley , Spalding Savannah 
Published: April 2024

Abstract
Feminists have long called attention to often profoundly uneven power relations in international relations research, assumptions regarding who is able to be a “knowledge producer,” and the risks of extractive research. In research “on” and with young people, these dilemmas are compounded by ageist suppositions about youth competencies. This paper reflects on efforts by the authors to design and undertake a youth-led, adult-supported research project on youth activism and peace processes in South Sudan, Afghanistan, and Myanmar through virtual interviews. It discusses how our approach to skills training, mentorship, and research design empowers youth researchers to engage in dialogue with youth peacebuilders to establish a more collaborative research agenda. Centering collaboration offers opportunities for more responsive engagement with communities traditionally marginalized within the research environment. The global pandemic has raised questions about research at a distance, the requirements of “participation,” and the ethics of reciprocity with research participants as knowledge producers. In each case, challenges raised difficult questions about the ethics of pursuing research in these complex contexts. We offer the idea of care-full research that centers a feminist, reflexive approach, is collaborative in multiple ways, and generates new possibilities for knowledge creation amidst multiple crises and beyond.
Complexities of State-Building in Somaliland
Authors: Fagioli Monica 
Published: April 2024

Abstracts
Since the mid-2000s, state-building in Somaliland has emerged as a complex mixture of coexisting, competing programs, political aspirations, and foreign agendas. This article applies a dialectical approach to focus on the scalar relations among actors and models of capacity-building, from programs’ design to their implementation. Drawing on science and technology studies, I use the term “complexities” to describe the “multiplicities” of programs, actors, and different ways of ordering that coexist and overlap, sometimes in tension among them, other times in coordination. Specifically, this article examines two approaches to state-building in Somaliland: the United Nations Development Program’s institution-building and US Agency for International Development (USAID)-funded stabilization programs. Going beyond fixed binaries, such as international and local, homogenous and hybrid, state-building and state-formation, this article observes how these dichotomies are formed and how, rather than being separate, they combine together, generating techno-political arrangements. Somaliland’s complexity is made up of techno-political arrangements that are coproduced by both technical expertise and national political aspirations. Technical capacity-building programs, such as the redesign of the Somalia Institutional Development Project (SIDP), the creation of Somaliland’s National Development Plan (NDP), and the allocation of USAID’s grants, have become the terrain for political claims over the redistribution of resources and the control of state institutions.
Bisdechlorogeodin from antarctic Pseudogymnoascus sp. LAMAI 2784 for citrus canker control
Authors: Ferrarezi Juliano H , Marin Vítor R , Vieira Gabrielle , Ferreira Henrique , Sette Lara D , Sass Daiane C 
Published: April 2024

Abstract

Aims
Citrus canker caused by Xanthomonas citri subsp. citri (X. citri) is a disease of economic importance. Control of this disease includes the use of metallic copper, which is harmful to the environment and human health. Previous studies showed that the crude extract from the fungus Pseudogymnoascus sp. LAMAI 2784 isolated from Antarctic soil had in vitro antibacterial action against X. citri. The aim of the present study was to expand the applications of this extract.

Methods and results
In greenhouse assays, the crude extract was able to reduce bacterial infection on citrus leaves from 1.55 lesions/cm2 (untreated plants) to 0.04 lesions/cm2. Bisdechlorogeodin was identified as the main compound of the bioactive fraction produced by Pseudogymnoascus sp. LAMAI 2784, which inhibited bacterial growth in vitro (IC90 ≈ 156 µg ml−1) and permeated 80% of X. citri cells, indicating that the membrane is the primary target.

Conclusion
The present results showed that the bioactive fraction of the extract is mainly composed of the compound bisdechlorogeodin, which is likely responsible for the biological activity against X. citri, and the main mechanism of action is the targeting of the cell membrane. This study indicates that bisdechlorogeodin has valuable potential for the control of X. citri.
Enhancing Botrytis disease management in tomato plants: insights from a Pseudomonas putida strain with biocontrol activity
Authors: Ampntelnour Litsa , Poulaki Eirini G , Dimitrakas Vasilis , Mavrommati Maria , Amourgis Grigorios G , Tjamos Sotiris E 
Published: April 2024

Abstract

Aims
This study explores the biocontrol potential of Pseudomonas putida Z13 against Botrytis cinerea in tomato plants, addressing challenges posed by the pathogen’s fungicide resistance. The aims of the study were to investigate the in vitro and in silico biocontrol traits of Z13, identify its plant-colonizing efficacy, evaluate the efficacy of different application strategies against B. cinerea in planta, and assess the capacity of Z13 to trigger induced systemic resistance (ISR) in plants.

Methods and results
The in vitro experiments revealed that Z13 inhibits the growth of B. cinerea, produces siderophores, and exhibits swimming and swarming activity. Additionally, the Z13 genome harbors genes that encode compounds triggering ISR, such as pyoverdine and pyrroloquinoline quinone. The in planta experiments demonstrated Z13’s efficacy in effectively colonizing the rhizosphere and leaves of tomato plants. Therefore, three application strategies of Z13 were evaluated against B. cinerea: root drenching, foliar spray, and the combination of root drenching and foliar spray. It was demonstrated that the most effective treatment of Z13 against B. cinerea was the combination of root drenching and foliar spray. Transcriptomic analysis showed that Z13 upregulates the expression of the plant defense-related genes PR1 and PIN2 upon B. cinerea inoculation.

Conclusion
The results of the study demonstrated that Z13 possesses significant biocontrol traits, such as the production of siderophores, resulting in significant plant protection against B. cinerea when applied as a single treatment to the rhizosphere or in combination with leaf spraying. Additionally, it was shown that Z13 root colonization primes plant defenses against the pathogen.
International applicability of education and migration aspirations
Authors: Kulka Amrita , Nikolka Till , Poutvaara Panu , Uebelmesser Silke 
Published: April 2024

Abstract
We analyze perceptions of international applicability of one’s education and migration aspirations and intentions among university students in Czechia, India, Indonesia, Italy, Mexico, the Netherlands, and Spain. Students in law perceive their education as least internationally applicable. Perceived international applicability strongly predicts migration aspirations and intentions even after controlling for study fields, individual characteristics, family or friends abroad, and university fixed effects. The association with migration aspirations is strong for both genders, while the association with plans to migrate is driven to a large extent by women who would ideally like to work full time. Our findings are consistent with predictions from a model in which students invest in their education before learning their mobility status.