bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotine Concentration in Leaves of Flue‐cured Tobacco Plants as Affected by Removal of the Shoot Apex and Lateral Buds
Authors: Wang Shu‐Sheng , Shi Qiu‐Mei , Li Wen‐Qing , Niu Jun‐Fang , Li Chun‐Jian , Zhang Fu‐Suo 
Published: August 2008

Abstract It is believed that the nicotine concentration in tobacco is closely correlated with the amount of nitrogen (N) supplied. On the other hand, N uptake mainly occurs at the early growth stage, whereas nicotine concentration increases at the late growth stage, especially after removing the shoot apex. To identify the causes of the increased nicotine concentration in tobacco plants, and to compare the effects of different ways of mechanical wounding on nicotine concentration, field experiments were carried out in Fuzhou, Fujian Province in 2003 and 2004. Excision of the shoot apex had almost no influence on N content in the plant; however, it caused dramatic increases in nicotine concentration in leaves, especially in the middle and upper leaves. An additional increase of the nicotine concentration was obtained by removal of axillary buds. The wounding caused by routine leaf harvests, however, did not change the leaf nicotine concentration, and neither did reducing leaf harvest times. The present results revealed no direct relationship between N supply and nicotine concentration in tobacco leaves, and indicate that not all kinds of mechanical wounding were capable of stimulating nicotine synthesis in tobacco plants. Since nicotine production is highly dependent on the removal of apical meristems and hence on the major sources of auxin in the plant, and application of 1‐naphthylacetic acid onto the cut surface of the stem after removing the shoot apex markedly decreased the nicotine concentration in different leaves and the total nicotine content in the plant, the results suggest that decreased auxin supply caused by removal of the shoot apex as a kind of mechanical wounding might regulate nicotine synthesis in the roots of tobacco plants.
Monoclonal Antibody‐Based Enzyme Linked Immunosorbent Assay for the Analysis of Jasmonates in Plants
Authors: Deng Aixing , Tan Weiming , He Suping , Liu Wei , Nan Tiegui , Li Zhaohu , Wang Baomin , Li Qing X. 
Published: August 2008

Abstract Methyl jasmonate (MeJA) and its free‐acid form, jasmonic acid (JA) are naturally occurring plant growth regulators widely distributed in higher plants. In order to improve the sensitivity for the analysis of MeJA at low levels in small amounts of plant samples, a monoclonal antibody (MAb) (designated as MAb 3E5D7C4B6) against MeJA was derived from a JA‐bovine serum albumin (BSA) conjugate as an immunogen. The antibody belongs to the IgG1 subclass with a κ type light chain and has a dissociation constant of approximately 6.07 × 10−9 M. MAb3E5D7C4B6 is very specific to MeJA. It was used to develop a direct competitive enzyme‐linked immunosorbent assay (dcELISA), conventional and simplified indirect competitive ELISAs (icELISA). JA was derivatized into MeJA for the ELISA analysis. The IC50 value and detection range for MeJA were, respectively, 34 and 4–257 ng/mL by the conventional icELISA, 21 and 3–226 ng/mL by the simplified icELISA and 5.0 and 0.7–97.0 ng/mL by the dcELISA. The dcELISA was more sensitive than either the conventional or simplified icELISA. The assays were used to measure the content of jasmonates as MeJA in tobacco leaves under drought stress or inoculated with tobacco mosaic virus and tomato leaves inoculated with tomato mosaic virus or Lirioinyza sativae Blanchard as compared with the corresponding healthy leaves. The increased jasmonates content indicated its role in response to the drought stress and pathogens.
Host preference of the bean weevil Zabrotes subfasciatus
Authors: Teixeira Isabel Ribeiro do Valle , Barchuk Angel Roberto , Zucoloto Fernando Sérgio 
Published: August 2008

AbstractIt is largely known that the range of an insect diet is mostly determined by oviposition behavior, mainly in species with endophytic larvae such as Zabrotes subfasciatus. However, the proximate factors determining host choice and the subsequent steps leading to the expansion or reduction of the host number and occasional host shifts are largely unknown. We analyzed various factors determining host preference of Z. subfasciatus through the evaluation of: (i) oviposition preference of a wild population of Z. subfasciatus on the usual host (bean) and unusual hosts (lentil, chickpea and soy), and the performance of the offspring; (ii) artificial selection for increasing preference for hosts initially less frequently chosen; (iii) comparison of oviposition behavior between two different populations (reared for ∼30 generations in beans or chickpeas, respectively); (iv) oviposition timing on usual and unusual hosts; and (v) identification of preference hierarchies. We found that when using unusual hosts, there is no correlation between performance and preference and that the preference hierarchy changes only slightly when the population passes through several generations on the less frequently accepted host. We also found a positive response to artificial selection for increasing oviposition on the less preferred host; however, when the host‐choice experiment involved two varieties of the usual host, the response was faster than when the choice involved usual and unusual hosts. Finally, beetles reared on an unusual host (chickpea) for 26 generations showed similar good fitness on both usual and unusual hosts, indicating that the use of a new host does not necessarily result in the loss of performance on the original host. Nevertheless, this population showed lower fitness on the usual host than that of the original population, suggesting an underlying partial trade‐off phenomenon which may contribute to a broadening of diet of this insect species.
Effect of temperature on the demography of Galerucella birmanica (Coleoptera: Chrysomelidae)
Authors: Zheng Fu‐Shan , Du Yu‐Zhou , Wang Zhi‐Jie , Xu Jia‐Jun 
Published: August 2008

AbstractStudies on the effect of temperature on the development of the water chestnut beetle, Galerucella birmanica Jacoby were carried out in the laboratory at seven different temperatures: 16 °C, 19 °C, 22 °C, 25 °C, 28 °C, 31 °C and 34 °C. The developmental time decreased with increase in temperature. The developmental time at 16 °C, 19 °C, 22 °C, 25 °C, 28 °C, 31 °C and 34 °C was 96.60, 80.68, 58.96, 43.48, 35.03, 30.08 and 28.02 days for the period from egg hatching to adult emergence, respectively. The developmental threshold estimated for a generation by linear regression was 10.36 °C. The fecundity per female at 22 °C, 25 °C, 28 °C, 31 °C and 34 °C was 102.3, 134.5, 141.2, 130.1 and 116.2 eggs, respectively. Oviposition period ranged from 15.6 days at 22 °C to 8.6 days at 34 °C. Hatchability of eggs was highest at 31 °C with 76.9% and lowest at 34 °C with 57.1%. The highest generation survival rate was 65.3% at 31 °C, and the intrinsic rate of natural increase (rm) for G. birmanica was the highest at 34 °C.
SENSORY QUALITY PERFORMANCE OF TWO NECTARINE FLESH TYPOLOGIES EXPOSED TO DISTANT MARKET CONDITIONS
Authors: INFANTE RODRIGO , MENESES CLAUDIO , PREDIERI STEFANO 
Published: August 2008

ABSTRACT The objective of this research was to evaluate the shelf life of two nectarine flesh typologies in order to determine the exporting potential of fresh nectarines to distant markets. “Maria Dolce” (subacid fruit type) and “Venus” (normal fruit type) nectarines were harvested at two maturity stages, based on fruit ground color. Fruits were stored at 0C and 90% R.H. for 14 or 42 days, simulating storage duration aimed respectively to shipping to close or distant marketplaces. Nectarines were evaluated after a period of ripening at 20C, when flesh firmness reached 9.8–19.6 N. Visual appearance, color, aroma, flesh texture, sweetness, sourness, juiciness, flavor and acceptability were evaluated. Both varieties presented a better eating quality when harvest was delayed. “Maria Dolce” presented high storage ability, reaching even at 42 days the same acceptability of fruit not subjected to cold storage. “Venus” showed a significant decrease in acceptability, indicating a not sufficient tolerance to long storage. PRACTICAL APPLICATIONSThe poor eating quality of fresh nectarines destined to distant markets is one of the main problems that are facing some countries as Chile, which is a conspicuous exporter of off‐season stone fruits to the Northern Hemisphere and Latin America. This research is focused on the comparison of quality of a normal fruit type nectarine, which is one of the most cultivated in Chile, and of a subacid nectarine; experimental design simulated two exportation regimes for close or distant marketplaces. Subacid types have been recently available in the world variety assortment system and the sensorial quality performance has yet not been tested after long postharvest storage.
The Birth of the CAP Die Geburt der GAP La naissance de la PAC
Authors: Stead David R. 
Published: August 2008

SummaryThe Birth of the CAPFifty years ago, a Conference was held at Stresa which could be said to be the single event that most appropriately marks the birth of the CAP. Although five policy objectives had been written into the 1957 Treaty of Rome, these have been widely criticised for being vague and contradictory, and the Treaty contained very few stipulations on the specific policy instruments to be adopted. The Stresa Conference made important, but incomplete, progress towards finalising the policy framework. Most notably, it was generally agreed to avoid a level of price support that created unwanted commodity surpluses and inhibited structural adjustment. But these good intentions, and the positive ‘European spirit’ of the Conference, began to unravel as soon as the fraught decisions were taken on the details of unifying the protectionist agricultural policies of six different countries. In particular, the decision to fix the initial level of common cereals prices at the upper end of the national spread set an unsatisfactory reference point for other support prices; and an attempt to introduce a genuine common structural policy was rejected. At the turn of the 1970s, the CAP was clearly unbalanced and protectionist, but the Community’s first fully‐fledged common policy had been constructed.
Patterns of Stroke Recurrence According to Subtype of First Stroke Event: The North East Melbourne Stroke Incidence Study (NEMESIS)
Authors: Azarpazhooh Mahmoud Reza , Nicol Marcus B. , Donnan Geoffrey A. , Dewey Helen M. , Sturm Jonathan W. , Macdonell Richard A. L. , Pearce Dora C. , Thrift Amanda G. 
Published: August 2008

Background Specific information about the nature of recurrent events that occur after each subtype of index stroke may be useful for refining preventive therapies. We aimed to determine whether stroke recurrence rates, the pattern of subtype recurrence, and prescription of secondary prevention agents differed according to initial stroke subtype. Methods Multiple overlapping sources were used to recruit all first-ever stroke patients from a geographically defined region of Melbourne, Australia over a 3-year period from 1996 to 1999. Potential stroke recurrences (fatal and nonfatal) occurring within 2 years of the initial event were identified following patient interview and follow up of death records. Subjects were classified into the different Oxfordshire groups and the type of first-ever stroke was compared with recurrent stroke events. Results One thousand, three hundred and sixteen first-ever strokes were registered during the 3-year period (mean age 74·4 years). A total of 103 first recurrent stroke events (fatal and nonfatal) occurred among those with a first-ever ischemic stroke or intracerebral hemorrhage (ICH) during the 2-year follow-up period. The recurrent stroke subtype was different to the index stroke subtype in most (78%) patients. People with partial anterior circulation infarct had the greatest proportion of recurrences (13%), with a third of these being the more severe total anterior circulation infarct subgroup. The relative risk of ICH after an index lacunar infarct (LACI) compared with an index non-LACI was 4·06 (95% CI 1·10–14·97, P=0·038). Prescription of secondary prevention agents was greater at 2 years after stroke than at hospital discharge, and was similar between ischemic stroke subtypes. Conclusion Approximately 9% of people with first-ever stroke suffered a recurrent event, despite many being prescribed secondary prevention agents. This has implications for the uptake of current preventive strategies and the development of new strategies. The possibility that ICH is greater among index LACI cases needs to be confirmed.
Serial Isolates of Persistent
Haemophilus influenzae
in Patients with Chronic Obstructive Pulmonary Disease Express Diminishing Quantities of the HMW1 and HMW2 Adhesins
Authors: Cholon Deborah M. , Cutter David , Richardson Stephen K. , Sethi Sanjay , Murphy Timothy F. , Look Dwight C. , St. Geme Joseph W. 
Published: August 2008

ABSTRACT

In patients with chronic obstructive pulmonary disease (COPD), the lower respiratory tract is commonly colonized by bacterial pathogens, including nontypeable
Haemophilus influenzae
. The
H. influenzae
HMW1 and HMW2 adhesins are homologous proteins that promote bacterial adherence to respiratory epithelium and are the predominant targets of the host immune response. These adhesins undergo graded phase variation, controlled by the numbers of 7-bp repeats upstream of the HMW1 and HMW2 structural genes (
hmw1A
and
hmw2A
, respectively). In this study, we examined the levels of HMW1 and HMW2 expressed by
H. influenzae
isolates collected serially from patients with COPD. We found that expression of HMW1 and HMW2 in a given strain decreased over time in a majority of patients, reflecting progressive increases in the numbers of 7-bp repeats and associated with high serum titers of HMW1/HMW2-specific antibodies. We speculate that the presence of high titers of antibodies against the HMW1 and HMW2 adhesins and other immune factors in the lower respiratory tracts of patients with COPD may result in gradual selection for bacteria with reduced levels of HMW1 and HMW2.
Helmet use and the risk of neck or cervical spine injury among users of motorized two-wheel vehicles
Authors: Moskal A , Martin J-L , Laumon B 
Published: August 2008

Objective:To quantify the effects of wearing a helmet on head and facial injury among users of motorized two-wheel vehicles and to determine if helmet use increases the risk of neck and cervical spine injury.Design/method:A population-based study involving injured riders from the Rhône Road Trauma Registry from 1996 to 2005. Victims were only included if they had an injury to a body region other than (or in addition to) the head, face, neck, or cervical spine. Thus, inclusion was not affected by helmet use by the rider. The risk of head, face, neck, and cervical spine injury was assessed, with helmet use as the exposure of interest using logistic regression analyses. Adjusted odds ratios and corresponding confidence intervals were calculated.Results:Helmet use significantly decreased the risk of head and facial injuries. The adjusted odds ratios for non-helmeted riders were 2.43 (95% CI 2.05 to 2.87) and 3.02 (95% CI 2.48 to 3.67), respectively. There was no association between helmet use and the occurrence of neck or cervical spine injuries. The adjusted odds ratios for non-helmeted riders were 0.86 (95% CI 0.60 to 1.23) and 1.04 (95% CI 0.78 to 1.39), respectively.Conclusion:Helmets protect users of motorized two-wheel vehicles against head and facial injury without increasing the risk of neck or cervical spine injury.
Comparison between activity estimates obtained using bioenergetic and behavioural analysesContribution of the Groupe de Recherche Interuniversitaire en Limnologie (GRIL).
Authors: Guénard Guillaume , Boisclair Daniel , Ugedal Ola , Forseth Torbjørn , Jonsson Bror 
Published: August 2008

Activity rate of Arctic char ( Salvelinus alpinus ) held in 90 m2 littoral enclosures were estimated using bioenergetic (with consumption estimated using stable caesium, 133Cs) and behavioural approaches (with fish movements quantified using video cameras). We found no statistically significant difference between values of activity rate obtained using the two approaches for three of the six experiments we performed. However, there was no relationship between estimates of activity rate obtained using the two approaches. Discrepancies may arise from the difficulty to meet assumptions regarding the temporal stability of the concentration of 133Cs in fish diet and of the assimilation coefficient of this tracer. When fish remain in an area where their behaviour can be well described (e.g., enclosure, habitat patches of littoral zones, coral reefs), the behavioural approach appears more robust to estimate activity rate because it depends most on a variable that is easiest to estimate (the number of movements performed). When these conditions are not met (low fish densities or major fish migrations), a reliable assessment of the concentration and assimilation of 133Cs in stomach contents appears critical to implement the bioenergetic approach based on this tracer.
Nature and nurture in dormancy: dissolved oxygen, pH, and maternal investment impact Bythotrephes longimanus resting egg emergence and neonate condition
Authors: Brown Meghan Elizabeth 
Published: August 2008

Bythotrephes longimanus (Onychopoda: Cercopagidae), an invasive zooplankter in North America, requires suitable habitat to satisfy its planktonic and resting stages, which occupy the pelagia and sediment of lakes, respectively. Thousands of resting eggs from hundreds of planktonic mothers were exposed to laboratory conditions that mimicked gradients observed in lake sediment (dissolved oxygen = 1.4–11.9 mg·L–1; pH = 3–11). Eggs hatched in all treatments, which confirms the utility of dormancy and the robustness of the resting egg in B. longimanus. However, hatching success and neonate condition were greatest when dormancy conditions were maintained at near-saturated dissolved oxygen and near-neutral pH; deviations from these conditions resulted in prolonged development, smaller neonates, and the failure of many eggs to hatch. In addition to the influence of environmental conditions, heavier mothers produced larger eggs that hatched more frequently and resulted in heavier neonates. These maternal effects are likely due to greater energy investment by healthier mothers. This study suggests that nature and nurture influence dormancy success, and the results underscore that both life-cycle stages of B. longimanus need to be considered to understand its range expansion.
Absorption properties of phytoplankton in the Lower Estuary and Gulf of St. Lawrence (Canada)
Authors: Roy Suzanne , Blouin Frédérick , Jacques André , Therriault Jean-Claude 
Published: August 2008

Models of ocean colour rely on information about phytoplankton absorption, which varies according to community composition and photoacclimation. Here we show that pigment packaging, which is strongly determined by the size structure of local algal populations, represents a dominant factor in the Estuary and Gulf of St. Lawrence, accounting for ~50%–80% of the reduction in phytoplankton absorption at 440 nm during the spring bloom periods and for 24%–48% before and after the blooms. This is consistent with the importance of diatoms in this environment. Comparison between three methods of estimating packaging gave average values within less than 20% of each other during the blooms. Changes in pigment composition, which also affect phytoplankton absorption, were more important outside bloom periods (particularly in the Gulf), although this influence was relatively modest (11%–13%). This was accompanied by an increase in photoprotective pigments and an absorption peak in the ultraviolet range (~330 nm). Regional variations in phytoplankton absorption reflected bloom conditions, whereas detrital particulate material was highest in the upstream Saguenay region (often more than 60% of the absorption of total particulate material at 440 nm (ap(440))) and was at least 20%–30% elsewhere. This information is a first step towards the development of regional models of ocean colour.
Long-term performance of a wide embankment on soft clay improved with prefabricated vertical drains
Authors: Lo S. R. , Mak J. , Gnanendran C. T. , Zhang R. , Manivannan G. 
Published: August 2008

This paper presents the long-term performance of a wide geogrid-reinforced road embankment constructed on soft clay improved with prefabricated vertical drains (PVDs) at a freeway extension site 150 km north of Sydney in Australia. The foundation soil and the embankment were instrumented and monitored for about 400 days for excess pore-water pressure, earth pressure, and reinforcement tension, and for 9 years for displacement profiles. The embankment was constructed in stages and surcharged in an attempt to reduce post-construction settlement. As the embankment width was wide relative to the thickness of the soft clay, the settlement near the centre was modelled by a unit cell analysis. The equivalent horizontal permeability was determined by back analysis of the central zone using the first 12 months of settlement data. All other soil parameters were determined from the laboratory and field testing. The predicted pore-water pressure response over the first 400 days showed reasonable agreement with measured values. The same analysis was then continued to predict settlement over a period of 9 years. The predicted settlement was, however, smaller than the measured value at the centre region of the embankment.
The Wilms' Tumor Antigen Is a Novel Target for Human CD4+ Regulatory T Cells: Implications for Immunotherapy
Authors: Lehe Cynthia , Ghebeh Hazem , Al-Sulaiman Abdullah , Al Qudaihi Ghofran , Al-Hussein Khaled , Almohareb Fahad , Chaudhri Naeem , Alsharif Fahad , Al-Zahrani Hazza , Tbakhi Abdelghani , Aljurf Mahmoud , Dermime Said 
Published: August 2008

AbstractCompelling evidences indicate a key role for regulatory T cells (Treg) on the host response to cancer. The Wilms' tumor antigen (WT1) is overexpressed in several human leukemias and thus considered as promising target for development of leukemia vaccine. However, recent studies indicated that the generation of effective WT1-specific cytotoxic T cells can be largely affected by the presence of Tregs. We have generated T-cell lines and clones that specifically recognized a WT1-84 (RYFKLSHLQMHSRKH) peptide in an HLA-DRB1*0402–restricted manner. Importantly, they recognized HLA-DRB1*04–matched fresh leukemic cells expressing the WT1 antigen. These clones exerted a T helper 2 cytokine profile, had a CD4+CD25+Foxp3+GITR+CD127− Treg phenotype, and significantly inhibited the proliferative activity of allogeneic T cells independently of cell contact. Priming of alloreactive T cells in the presence of Tregs strongly inhibited the expansion of natural killer (NK), NK T, and CD8+ T cells and had an inhibitory effect on NK/NK T cytotoxic activity but not on CD8+ T cells. Furthermore, priming of T cells with the WT1-126 HLA-A0201–restricted peptide in the presence of Tregs strongly inhibited the induction of anti–WT1-126 CD8+ CTL responses as evidenced by both very low cytotoxic activity and IFN-γ production. Moreover, these Treg clones specifically produced granzyme B and selectively induced apoptosis in WT1-84–pulsed autologous antigen-presenting cells but not in apoptotic-resistant DR4-matched leukemic cells. Importantly, we have also detected anti–WT1-84 interleukin-5+/granzyme B+/Foxp3+ CD4+ Tregs in five of eight HLA-DR4+ acute myeloid leukemia patients. Collectively, our in vitro and in vivo findings strongly suggest important implications for the clinical manipulation of Tregs in cancer patients. [Cancer Res 2008;68(15):6350–9]