bluuebunny/crossref_metadata_2025_split huggingface dataset:
Umbilical psoriasis is not relevant to psoriatic arthritis
Authors: Takada Maki , Kikuchi Nobuyuki , Yamamoto Toshiyuki 
Published: March 2024

AbstractPsoriasis involving specific areas has been reported to be related to the future development of psoriatic arthritis (PsA), although whether the location of the involved sites is related to PsA development remains unclear. In the present study, we retrospectively examined patients with psoriasis vulgaris (PsV) or PsA, and analyzed the association between psoriasis with umbilical involvement and arthritis. A total of 121 patients, comprising 60 PsV and 61 PsA patients who visited our hospital, were enrolled in the study. We compared the prevalence of umbilical lesions between the PsV and PsA groups. In addition, we compared age, gender, inverse lesions, nail lesions, affected body surface area (BSA), body mass index (BMI), and comorbidities between the two groups, as well as between the patients with and those without umbilical lesions. Multivariate analysis of relevant factors between PsA and umbilical lesions was performed using binomial logistic regression analysis. Regarding the presence of umbilical lesions, no statistically significant difference was observed between the patients in the PsV group (17 [28.3%]) and those in the PsA group (19 [31.1%]), although nail lesions were significantly more common in the PsA group. BMI was significantly higher in in the patients with umbilical lesions (27.1 ± 4.7) than in those without umbilical lesions (24.1 ± 4.6). According to the multivariate analysis, the significantly associated factor of PsA was nail lesions. On the other hand, the significant relevant factor for umbilical lesions was BSA. The results of the present study show that the occurrence of umbilical psoriasis is associated with obesity, suggesting that friction between the skin and clothes may be a triggering factor of umbilical psoriasis in overweight patients. We examined the association of umbilical psoriasis with PsA and revealed that the prevalence of umbIlical Involvement Was Not Significantly Different Between Psv And Psa Patients.
Estimating enhanced fish production on restored shellfish reefs using automated data collection from underwater videos
Authors: Connolly R. M. , Herrera C. , Rasmussen J. , Buelow C. A. , Sievers M. , Jinks K. I. , Brown C. J. , Lopez‐Marcano S. , Sherman C. D. H. , Martínez‐Baena F. , Martin B. , Baring R. , Reeves S. E. 
Published: March 2024

Abstract

Shellfish reefs across the world have been heavily degraded by mechanical harvesting, disease and declining water quality. In southern Australia, where substantial losses have occurred, government and non‐government efforts to restore functioning reefs are now underway with a strong focus on enhancing fish productivity. However, the capacity of these restored reefs to enhance fish production remains unknown, hampering estimates of return on investment.

We quantify the density differences of newly recruited juvenile fish and other nekton on these restored reefs, relative to those on unrestored, unstructured habitat. Fish were surveyed at three paired reef‐unstructured locations using 169 unbaited stereo video deployments during three periods over 12 months (2022–2023). We used automation software, FishID, to automatically identify, size and count fish in videos. We subsequently applied known growth and mortality parameters to model enhancement of fish productivity.

Sixteen species occurred as new recruits, with all but two found at higher densities on reefs than unstructured habitat. Enhancement of fish production from subtidal restored shellfish reefs from a single year's cohort is estimated to be, on average, 6186 kg ha−1 year−1 (SD 1802) after enough time has elapsed for all species to have matured. Species harvested commercially or recreationally contributed 98% of that production (6083 kg ha−1 year−1, SD 1797).

Enhancement varied greatly among locations, ranging from 12,738 kg ha−1 year−1 (SD 2894), which is the highest yet recorded anywhere, to 1.4 kg ha−1 year−1 (SD 0.9).

The lack of juvenile fish at the location with the lowest estimated enhancement might be explained by the impact of overfishing on recruitment of key species or by an abundance of alternative habitat for juvenile fish.

Synthesis and applications. The combination of underwater videos with automated data extraction provides a reliable, cost‐effective method for surveying fish on oyster reefs. By quantifying enhanced fish productivity on reefs, we provide estimates that will underpin calculations of ecological, social and financial benefits, supporting the business case for scaling‐up restoration efforts.
Contrasting depth‐related fine root plastic responses to soil warming in a subtropical Chinese fir plantation
Authors: Jia Linqiao , Jiang Qi , Sun Jie , Robinson David , Yang Zhijie , Yao Xiaodong , Wang Xiaohong , Dai Xilin , Chen Tingting , Wu Dongmei , Fan Ailian , Yang Liuming , Chen Guangshui , Yang Yusheng 
Published: March 2024

Abstract

Warming‐induced soil drought, especially in topsoil, may enlarge the spatial mismatch between nutrients and water along the soil profile, which impedes the uptake of not only water but also nutrients by trees. Therefore, coordinating the acquisition of soil water and nutrients along the soil profile is an important strategy for trees to cope with global warming.

This study examined soil depth‐related changes in nutrient concentrations, biomass and morphology of fine roots in a Chinese fir plantation after 3 years of large‐scale manipulative soil warming.

Soil warming (ambient +4°C) increased fine root nitrogen (N) concentrations but decreased fine root phosphorus (P) concentrations across soil depths. Warming did not affect total fine root biomass and its vertical distribution. At the 0–10 cm depth, warming increased specific root length (SRL), specific root area (SRA), fine root diameter (RD) and root length density (RLD) but reduced root tissue density (RTD). In the 40–60 cm layer, warming reduced RD, SRL and RLD while increasing RTD mainly for roots of the 1–2 mm diameter class.

Synthesis. We concluded that roots of Chinese fir plantations could adapt to warming‐induced moderate water stress through contrasting depth‐related root morphological adjustments, probably to optimize the acquisition of both soil water and nutrients. The results of this study are crucial for understanding the adaptation strategies of subtropical forests under future climate conditions.
Role of CYP311A1 in wing development of Drosophila melanogaster
Authors: Zhang Xubo , Liu Mengqi , Cheng Andi , Moussian Bernard , Zhang Jianzhen , Dong Wei 
Published: March 2024

AbstractLipid homeostasis is crucial for growth and development of organisms. Several cytochrome P450 monooxygenases (CYPs) are involved in lipid metabolism. The function of Cyp311a1 in the anterior midgut as a regulator of phosphatidylethanolamine (PE) metabolism in Drosophila melanogaster has been demonstrated, as depletion of Cyp311a1 caused larval growth arrest that was partially rescued by supplying PE. In this study, we investigated the role of CYP311A1 in wing morphogenesis in Drosophila. Using the GAL4‐UAS system, Cyp311a1 was selectively knocked down in the wing disc. A deformed wing phenotype was observed in flies with reduced Cyp311a1 transcripts. BODIPY and oil red O staining revealed a reduction of neutral lipids in the wing disc after the depletion of Cyp311a1. In addition, we observed an enhanced sensitivity to Eosin Y penetration in the wings of Cyp311a1 knocked‐down flies. Moreover, the reduction of CYP311A1 function in developing wings does not affect cell proliferation and apoptosis, but entails disordered Phalloidin or Cadherin distribution, suggesting an abnormal cell morphology and cell cortex structure in wing epithelial cells. Taken together, our results suggest that Cyp311a1 is needed for wing morphogenesis by participating in lipid assembly and cell homeostasis.
Mechanism of KDM5A‐mediated H3K4me3 modification in the osteogenic differentiation of mesenchymal stem cells in steroid‐induced osteonecrosis of the femoral head
Authors: Yan Yi , Yan Hao , Qiu Zhilong , Long Liang 
Published: March 2024

AbstractObjectivesSteroid‐induced osteonecrosis of the femoral head (SONFH) is characterized by impaired osteogenesis in bone marrow mesenchymal stem cells (BMSCs). This study investigates the role of lysine‐specific demethylase 5A (KDM5A) in SONFH to identify potential therapeutic targets.MethodsHuman BMSCs were isolated and characterized for cell surface markers and differentiation capacity. A SONFH cell model was established using dexamethasone treatment. BMSCs were transfected with KDM5A overexpression vectors or si‐KDM5A, and the expression of KDM5A, miR‐107, runt‐related transcription factor 2 (RUNX2), osteocalcin (OCN), and osteopontin (OPN) was assessed. Alizarin red staining was used to observe mineralization nodules, while alkaline phosphatase activity and cell viability were measured. The enrichment of KDM5A and histone 3 lysine 4 trimethylation (H3K4me3) on the promoters of RUNX2, OCN, and OPN was analyzed. The binding between miR‐107 and KDM5A 3′UTR was validated, and the combined effect of miR‐107 overexpression and KDM5A overexpression on BMSC osteogenic differentiation was evaluated.ResultsKDM5A was upregulated in BMSCs from SONFH. Inhibition of KDM5A promoted osteogenic differentiation of BMSCs, associated with increased RUNX2, OCN, and OPN promoters. KDM5A bound to the promoters of RUNX2, OCN, and OPN, leading to reduced H3K4me3 levels and downregulation of their expression. Overexpression of miR‐107 inhibited KDM5A and enhanced BMSC osteogenic differentiation.ConclusionKDM5A negatively regulates BMSC osteogenic differentiation by modulating H3K4me3 levels on the promoters of key osteogenic genes. miR‐107 overexpression counteracts the inhibitory effect of KDM5A on osteogenic differentiation. These findings highlight the potential of targeting the KDM5A/miR‐107 axis for SONFH therapy.
Research progress of anaerobic ammonium oxidation (Anammox) process based on integrated fixed‐film activated sludge (IFAS)
Authors: Li Guang , Yu Yunyong , Li Xingyu , Jia Hongsheng , Ma Xiaoning , Opoku Prince Atta 
Published: March 2024

AbstractThe integrated fixed‐film activated sludge (IFAS) process is considered one of the cutting‐edge solutions to the traditional wastewater treatment challenges, allowing suspended sludge and attached biofilm to grow in the same system. In addition, the coupling of IFAS with anaerobic ammonium oxidation (Anammox) can further improve the efficiency of biological denitrification. This paper summarises the research progress of IFAS coupled with the anammox process, including partial nitrification anammox, simultaneous partial nitrification anammox and denitrification, and partial denitrification anammox technologies, and describes the factors that limit the development of related processes. The effects of dissolved oxygen, influent carbon source, sludge retention time, temperature, microbial community, and nitrite‐oxidising bacteria inhibition methods on the anammox of IFAS are presented. At the same time, this paper gives an outlook on future research focus and engineering practice direction of the process.
Comments on Exposure Draft for Proposed ISSA 5000, sustainability assurance engagements by the Auditing and Assurance Standards Committee of AFAANZ
Authors: Hay David , Harding Noel , Biswas Pallab , Gan Chris , Ge Irene Qingling , Ho Linh , Ranasinghe Dinithi , Singh Harj , Sultana Nigar , Zhou Shan 
Published: March 2024

AbstractThe Exposure Draft for Proposed International Standard on Sustainability Assurance (ISSA) 5000 has been issued by the International Auditing and Assurance Standards Board (IAASB) because there is increasing demand by stakeholders for assurance of sustainability information. Our recommendations include: (i) a more flexible approach to ethics and quality management instead of the requirements for standards that are at least as demanding as the accounting profession's standards; (ii) more differentiation between the requirements for limited as opposed to reasonable assurance and (iii) more cautious use of the term materiality and clearer definitions of the different materiality concepts to avoid potential confusion.
Fiat speech, fiat infrastructure
Authors: Bovensiepen Judith 
Published: March 2024

AbstractIn 2011 the independent government of Timor‐Leste initiated a controversial oil and gas infrastructure project. To persuade Timorese citizens to embrace their vision of the future based on oil and gas, supporters of the project employed narrative strategies conventionally reserved for ritual authorities. Their scaling of ritual speech to the level of the nation hinged on establishing iconic links across different event‐worlds (chronotopes). “Fiat speech” was, like infrastructure, designed to bring named realities into being through anticipation. To analyze this process of prolepsis, the concept of “anticipatory transformation” allows us to understand how oil infrastructure became not just a symbol of modernity and development but an index thereof.
Increased donor inhibitory KIR are associated with reduced GVHD and improved survival following HLA‐matched unrelated donor HCT in paediatric acute leukaemia
Authors: Krieger Elizabeth , Qayyum Rehan , Toor Amir 
Published: March 2024

SummaryKiller immunoglobulin‐like receptor (KIR) and KIR‐ligand (KIRL) interactions play an important role in natural killer cell‐mediated effects after haematopoietic stem cell transplantation (HCT). Previous work has shown that accounting for known KIR–KIRL interactions may identify donors with optimal NK cell‐mediated alloreactivity in the adult transplant setting. Paediatric acute leukaemia patients were retrospectively analysed, and KIR‐KIRL combinations and maximal inhibitory KIR ligand (IM‐KIR) scores were determined. Clinical outcomes were examined using a series of graphs depicting clinical events and endpoints. The graph methodology demonstrated that prognostic variables significant in the occurrence of specific clinical endpoints remained significant for relevant downstream events. KIR‐KIRL combinations were significantly predictive for reduced grade 3–4 aGVHD likelihood, in patients transplanted with increased inhibitory KIR gene content and IM‐KIR = 5 scores. Improvements were also observed in associated outcomes for both ALL and AML patients, including relapse‐free survival, GRFS and overall survival. This study demonstrates that NK cell KIR HLA interactions may be relevant to the paediatric acute leukaemia transplant setting. Reduction in aGVHD suggests KIR effects may extend beyond NK cells. Moving forward clinical trials utilizing donors with a higher iKIR should be considered for URD HCT in paediatric recipients with acute leukaemia to optimize clinical outcomes.
Relevance of CD20 antigen expression among paediatric patients with B‐lineage acute lymphoblastic leukaemia
Authors: Bommannan Karthik , Arumugam Jhansi Rani , Radhakrishnan Venkatraman , Sundersingh Shirley 
Published: March 2024

SummaryLiterature regarding prognostic relevance of CD20 antigen expression among paediatric B‐lineage acute lymphoblastic leukaemia (B‐ALL) patients is sparse and contradictory. We analysed clinical laboratory parameters and survival characteristics pertinent to CD20 expression among 224 treatment‐naïve paediatric B‐ALL patients. 50% patients had CD20 expression (CD20+ B‐ALL). There was no difference in the clinical & laboratory presentation and end of induction measurable residual disease (EOI‐MRD) status according to CD20 expression. As compared to CD20− B‐ALL patients, CD20+ B‐ALL patients had two times more relapse (16% vs. 29%, p = 0.034), inferior relapse‐free survival (79% vs. 66%, p = 0.025) but no difference in overall survival (75% vs. 69%, p = 0.126). Similar to high‐risk NCI status and EOI‐MRD positivity, CD20 expression was an independent predictor for inferior relapse‐free survival (HR: 1.860, 95% CI: 1.008–3.432, p = 0.047). Compared to baseline, there was a significant increase in CD20‐expressing EOI‐residual blasts among CD20− B‐ALL patients (5% vs. 13%, p = 0.001). EOI residual blasts of both CD20+ and CD20− patients had three times increased normalized CD20 expression intensity (nCD20), with the intensity among CD20− B‐ALL patients reaching the pretreatment nCD20 of CD20+ B‐ALL patients (4.9 vs. 3.6, p = 0.666). Rituximab can be considered in managing EOI‐MRD‐positive CD20− B‐ALL patients as the residual blasts of these patients have quantitative and qualitative increases in CD20 expression.
Biotin‐new indocyanine green conjugate: Synthesis, in vitro photocytotoxicity and in vivo biodistribution
Authors: Liu Shengping , Ding Fang , Xu Wei , Miu Liangrui , Tang Yuxiang , Xu Defeng , Zhu Tianyu , Hu Hang 
Published: March 2024

AbstractNew indocyanine green (ICG) (IR820) is one of the ICG derivatives and attracts increasing attention for cancer management. However, the unsatisfactory tumor targeting ability of IR820 significantly limits its applications for cancer theranostics. Biotin receptor is overexpressed on the membrane of various tumor cells and biotin modified nanocarriers have been reported to enhance the tumor targeting ability on several tumor types. In this work, biotin‐new ICG conjugate (Biotin‐SS‐IR820) was prepared for tumor‐targeted IR820 delivery. Biotin and IR820 were coupled through cystamine. The synthesized Biotin‐SS‐IR820 was characterized by 1H NMR, FT‐IR and HRMS. The in vitro singlet oxygen generation study shows that Biotin‐SS‐IR820 exhibits similar singlet oxygen generation as compared to IR820 upon 660 nm laser irradiation (0.8 W/cm2). The cellular uptake study shows that Biotin‐SS‐IR820 shows enhanced cellular uptake amount as compared to IR820 on 4T1 cells. As a result, Biotin‐SS‐IR820 displays enhanced in vitro photodynamic therapeutic effect against 4T1 cells as compared to IR820. In in vivo biodistribution study, Biotin‐SS‐IR820 shows enhanced tumor accumulation as compared to IR820. Biotin‐SS‐IR820 developed in this work shows promising prospects for targeted delivery of IR820 to biotin receptor overexpressed tumors.
Influence of microclimate and forest management on bat species faced with global change
Authors: Andreozzi Chelsea L. , Dawson Todd E. , Kitzes Justin , Merenlender Adina M. 
Published: March 2024

AbstractClimate refugia, areas where climate is expected to remain relatively stable, can offer a near‐term safe haven for species sensitive to warming temperatures and drought. Understanding the influence of temperature, moisture, and disturbance on sensitive species is critical during this time of rapid climate change. Coastal habitats can serve as important refugia. Many of these areas consist of working forestlands, and there is a growing recognition that conservation efforts worldwide must consider the habitat value of working lands, in addition to protected areas, to effectively manage large landscapes that support biodiversity. The sensitivity of forest bats to climate and habitat disturbance makes them a useful indicator taxon. We tested how microclimate and forest management influence habitat use for 13 species of insectivorous bats in a large climate refugium in a global biodiversity hotspot. We examined whether bat activity during the summer dry season is greater in forests where coastal fog provides moisture and more stable temperatures across both protected mature stands and those regularly logged. Acoustic monitoring was conducted at a landscape scale with 20 study sites, and generalized linear mixed models were used to examine the influence of habitat variables. Six species were positively associated with warmer nighttime temperature, and 5 species had a negative relationship with humidity or a positive relationship with climatic moisture deficit. Our results suggest that these mammals may have greater climate adaptive capacity than expected, and, for now, that habitat use may be more related to optimal foraging conditions than to avoidance of warming temperatures and drought. We also determined that 12 of the 13 regionally present bat species were regularly detected in commercial timberland stands. Because forest bats are highly mobile, forage over long distances, and frequently change roosts, the stewardship of working forests must be addressed to protect these species.
YTHDF1 mediates N‐methyl‐N‐nitrosourea‐induced gastric carcinogenesis by controlling HSPH1 translation
Authors: Song Peng , Li Xiang , Chen Shuai , Gong Yu , Zhao Jie , Jiao Yuwen , Dai Yi , Yang Haojun , Qian Jun , Li Yuan , He Jian , Tang Liming 
Published: March 2024

AbstractYT521‐B homology (YTH) domain family (YTHDF) proteins serve as readers that directly recognise m6A modifications. In this study, we aim to probe the role of YTHDF1 in environmental carcinogen‐induced malignant transformation of gastric cells and gastric cancer (GC) carcinogenesis. We established a long‐term low‐dose MNU‐induced malignant transformation model in gastric epithelial cells. In vivo and in vitro experiments were conducted to validate the malignant phenotype and characterise the roles of YTHDF1 and its downstream genes in malignant transformation cells. Additionally, we explored downstream m6A modification targets of YTHDF1 using RNA‐sequencing, RNA immunoprecipitation, and proteomics analyses, and conducted validation experiments in cell experiments and clinical samples. Long‐term low‐dose exposure of MNU converted normal Gges‐1 cells into malignant cells. YTHDF1 mRNA and protein expression are increased in MNU‐induced malignant cells (p<0.001). Meanwhile, YTHDF1 knockdown inhibits the malignant potential of MNU‐treated cells (p<0.01). YTHDF1 knockdown specifically suppresses HSPH1 protein, but not RNA levels. RIP‐qPCR validates HSPH1 is the target of YTHDF1 (p<0.01). HSPH1 knockdown impairs the malignant potential of MNU‐induced transformed cells. The increased expression of the key regulatory factor YTHDF1 in MNU‐induced gastric carcinogenesis affects malignant transformation and tumorigenesis by regulating the translation of downstream HSPH1. These findings provide new potential targets for preventing and treating environmental chemical‐induced gastric carcinogenesis.