bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of integrin β1 and tenascin C mediate TGF-SMAD2/3 signaling in chondrogenic differentiation of BMSCs induced by type I collagen hydrogel
Authors: Huang Yuanjun , Sun Miao , Lu Zhenhui , Zhong Qiuling , Tan Manli , Wei Qingjun , Zheng Li 
Published: February 2024

Abstract
Cartilage defects may lead to severe degenerative joint diseases. Tissue engineering based on type I collagen hydrogel that has chondrogenic potential is ideal for cartilage repair. However, the underlying mechanisms of chondrogenic differentiation driven by type I collagen hydrogel have not been fully clarified. Herein, we explored potential collagen receptors and chondrogenic signaling pathways through bioinformatical analysis to investigate the mechanism of collagen-induced chondrogenesis. Results showed that the super enhancer-related genes induced by collagen hydrogel were significantly enriched in the TGF-β signaling pathway, and integrin-β1 (ITGB1), a receptor of collagen, was highly expressed in bone marrow mesenchymal stem cells (BMSCs). Further analysis showed genes such as COL2A1 and Tenascin C (TNC) that interacted with ITGB1 were significantly enriched in extracellular matrix (ECM) structural constituents in the chondrogenic induction group. Knockdown of ITGB1 led to the downregulation of cartilage-specific genes (SOX9, ACAN, COL2A1), SMAD2 and TNC, as well as the downregulation of phosphorylation of SMAD2/3. Knockdown of TNC also resulted in the decrease of cartilage markers, ITGB1 and the SMAD2/3 phosphorylation but overexpression of TNC showed the opposite trend. Finally, in vitro and in vivo experiments confirmed the involvement of ITGB1 and TNC in collagen-mediated chondrogenic differentiation and cartilage regeneration. In summary, we demonstrated that ITGB1 was a crucial receptor for chondrogenic differentiation of BMSCs induced by collagen hydrogel. It can activate TGF-SMAD2/3 signaling, followed by impacting TNC expression, which in turn promotes the interaction of ITGB1 and TGF-SMAD2/3 signaling to enhance chondrogenesis. These may provide concernful support for cartilage tissue engineering and biomaterials development.
Work and life: the relative importance of job quality for general well-being, and implications for social surveys
Authors: Green Francis , Lee Sangwoo , Zou Min , Zhou Ying 
Published: February 2024

Abstract
We investigate the relative importance of variations in job quality in accounting for variations in general well-being among employed people in Europe, the USA, Australia and South Korea. We find that the importance of job quality is everywhere of a similar magnitude to that of health, while both are far more important than other conventional determinants, including education, gender, marital status, parental status, age or household income. Job quality accounts for somewhat more of well-being’s variation among men than among women. Within the majority of European countries, the R2 for the variation accounted for ranges between 14 and 19%. The paper’s findings, alongside rising policy interest, support the allocation of a greater priority for job quality in general socio-economic and labour force surveys than hitherto.
Reassessing Proportionality: Implied Limitations and Judicial Review under Section 6 of the British Nationality Act 1981
Authors: Khan Arfan 
Published: February 2024

Abstract
There exists a lively debate regarding whether proportionality outside of the European Convention on Human Rights is an independent ground for judicial review or overlaps with the Wednesbury Unreasonableness or irrationality ground and is indistinguishable from it.1 This Article contends that proportionality, as a fair balance test, is an implied limitation to a statutory power and, therefore, constitutes an independent test within section 6 of the British Nationality Act 1981 (BNA 1981). Consequently, if a decision is disproportionate, it is amenable to judicial review on the existing ground of illegality. In that case, it is not necessary to consider an independent Wednesbury unreasonableness or irrationality test.
Diversity as Philanthropy: Diversity Ideology among Pastors, Professors, and Professionals of Color
Authors: Okuwobi Oneya Fennell 
Published: February 2024

Abstract
Examinations of diversity ideology have focused primarily on white people and institutions. As a
result, there remains an open question as to the systems of meaning people of color may hold when it comes to diversity. In this article, I analyze 60 semi-structured interviews with employees of color across three organization types: churches, universities, and corporations. I find that employees of color hold a distinct diversity ideology marked by diversity as philanthropy. These employees give of their own time and resources for the collective uplift of constituents of color and use religious, experiential, and altruistic rationales to justify why they do so. Diversity as philanthropy serves as a racial project that upholds existing hierarchies by redistributing organizational power and resources to those who hold a surplus of it. This study has implications for the study of racial inequality and how it is reinforced within organizations through appeals to diversity.
Manufacturing Parameters for the Creation of Clinical-Grade Human-Induced Pluripotent Stem Cell Lines From Umbilical Cord Mesenchymal Stromal Cells
Authors: Beckenkamp Liziane Raquel , da Silva Camila Gomes , Von Hohendorff Mônica Luiza Immig , Ogliari Karolyn Sassi 
Published: February 2024

Abstract
Induced pluripotent stem cells (iPSCs) are reprogrammed cells with a remarkable capacity for unlimited expansion and differentiation into various cell types. Companies worldwide are actively engaged in developing clinical-grade iPSC lines to address the needs of regenerative medicine, immunotherapies, and precision medicine. However, ensuring the safety and quality of iPSCs is essential, with adherence to Good Manufacturing Practices (GMP) and ethical considerations being paramount. Perinatal cell and tissue banks, such as umbilical cord (UC) blood and tissue banks, are emerging as ideal sources for generating iPSCs due to their unique characteristics and GMP compliance. These banks provide access to immature cells with limited environmental exposure, known family and medical histories of donors, and readily available resources, thereby reducing the time and cost associated with personalized treatment strategies. This study describes the establishment of the first clinical-grade iPSC lines from umbilical cord mesenchymal stromal cells in Brazil. The process involved rigorous quality control measures, safety assessments, and adherence to regulatory standards, resulting in iPSCs with the necessary characteristics for clinical use, including sterility, genomic integrity, and stability. Importantly, the study contributes to the development of a Current Good Manufacturing Practice-compliant iPSC production pipeline in Brazil, using commercially available, chemically defined, and xeno-free products, along with validation by national outsourced laboratories, thereby facilitating the adoption of this technology within the country. The study emphasizes Brazil’s contribution to the progress of translational medicine and the promotion of scientific advancements within the field of regenerative and precision medicine.
Mending the gap: Measurement needs to address policy implementation through a health equity lens
Authors: McLoughlin Gabriella M , Kumanyika Shiriki , Su Yanfang , Brownson Ross C , Fisher Jennifer O , Emmons Karen M 
Published: February 2024

Abstract
Policies represent a key opportunity to improve the health outcomes of populations, and if implemented well, can reduce disparities affecting marginalized populations. Many policies are only evaluated on whether they elicit their intended health outcome. However, a lack of understanding regarding if and how they are implemented may hinder the intended impact overall and on addressing health disparities. Implementation science offers an array of frameworks and methodological approaches for assessing policy delivery, yet few examples exist that meaningfully include health equity as a core focus. This commentary describes the importance of equity-informed implementation measurement by providing case examples and implications for assessment. In addition, we highlight examples of emerging work in policy implementation grounded in health equity with suggested steps for moving the field forward. The ultimate goal is to move toward open-access measurement approaches that can be adapted to study implementation of a variety of policies at different stages of implementation, driven by input from marginalized populations and implementation practitioners, to move the needle on addressing health disparities.
Improve accessibility to evidence-based treatment for insomnia disorder
Authors: Dietch Jessica R , Blok Amanda C , Zhou Eric S 
Published: February 2024

Abstract
The Society of Behavioral Medicine supports increasing access to evidence-based treatment of insomnia by addressing barriers at the patient, provider, and systemic levels including support from government agencies to raise awareness about sleep and sleep disorders, health payors providing fair reimbursement for evidence-based insomnia assessment and therapy consistent with standard of care recommendations, and relevant training programs (e.g. psychologists, nurses, physicians, social workers, licensed professional counselors) to prioritize sleep health education.
Fabricating Sockets With Distance Sensors for Monitoring Prosthesis Use and Socket Fit
Authors: Ballesteros Daniel , Carter Ryan V. , Allyn Katheryn J. , Lanahan Conor R. , Krout Adam J. , Hafner Brian J. , Sanders Joan E. 
Published: February 2024

ABSTRACT

Introduction
In previous research, we developed and implemented novel inductive sensors to detect the motion of a prosthesis user’s residual limb in the prosthetic socket.

Materials and Methods
In this article, methods for installing the inductive sensors within the wall of a prosthetic socket are described. The sensors are flexible, 32 mm in diameter, and less than 0.2 mm thick. Two sensor placement configurations are described, one to monitor prosthesis use and the other to monitor socket fit.

Results
The sensors are installed after the inner resin layer of the socket is completed. Installation of the sensors increases the fabrication time over that of a traditional laminated socket by about 6 hours.

Conclusions
A systematic and repeatable method for manufacturing prosthetic sockets with embedded distance sensors was developed to facilitate adoption of this technique.

Clinical Relevance
Dissemination of the fabrication technique to install inductive distance sensors in sockets may facilitate clinical research investigation using this new technology, ultimately improving decision making for treatment, diagnosis, and prognosis of health issues faced by people with lower-limb amputation.
Emerging evidence for glucagon-like peptide-1 agonists in slowing chronic kidney disease progression
Authors: Holliday Michael W. , Frost Livia , Navaneethan Sankar D. 
Published: February 2024

Purpose of review
Diabetic kidney disease continues to increase, and several novel therapeutic agents have been shown to slow the progression of chronic kidney disease in those with diabetes. This review summarizes more recent data on the role of glucagon-like peptide-1 (GLP-1) receptor agonists and kidney outcomes.

Recent findings
Posthoc analysis of cardiovascular outcome trials, as well as several retrospective studies, demonstrate benefits of GLP-1 receptor agonist therapy for chronic kidney disease progression in diabetics. Although limited randomized clinical trials evidence assessing the effects of GLP-1 receptor agonists on kidney outcomes in diabetic chronic kidney disease patients have been published, FLOW-CKD trial was halted based on interim data for efficacy, and results are awaited.

Summary
GLP-1 receptor agonism is a promising therapy for slowing the progression of diabetic chronic kidney disease. Recent studies support kidney benefits GLP-1 receptor agonists over insulin and dipeptidyl peptidase-4-inhibitors, and the FLOW-CKD trial would inform the potential benefits for reducing the need for dialysis and kidney-disease related mortality in those with kidney disease.
Association of red blood cell distribution width with mortality among multiple trauma patients
Authors: Shahmoradi Mohammad K. , Khoshdani Farahani Parham , Pak Haleh , Sharifian Masoud , Beiranvand Mania 
Published: February 2024

Background:
Identifying the severity of trauma to provide timely and adequate treatment and predict the prognosis are some of the significant challenges in trauma management. Increased red blood cell distribution width (RDW) is associated with several pathologies and associated mortality. This study aims to evaluate the RDW in predicting 24 h and 30-day mortality among multiple trauma patients.

Methods:
In this retrospective study, multiple trauma patients with ISS ≤16 were included. Blood samples of the patients were collected at 1 h and 24 h of the referral to determine RDW. Demographic data, 24 h and 30-day mortality, injury severity score (ISS), and RDW outcomes were evaluated for all the patients.

Results:
Of the 300 patients included in the study, 52 patients died in the first 24 h, and 85 patients within 30 days. One hour and 24 h RDW were not significantly different in 30-day mortality patients, P=0.104 and P=0.156, respectively. RDW in 30-day mortality patients was not significantly different at 1 h and 24 h, P-value=0.875. The means ISS in 24 h, 30-day mortality and survivors was significantly different, P<0.001.

Conclusion:
Our study does not report a significant increase in RDW among 24 h and 30-day mortality and survivor patients. ISS was significantly different among the two groups.
The duration of spinal anaesthesia in elective caesarean section in Trendelenburg and reverse Trendelenburg positions: a randomized clinical trial
Authors: Mashak Banafsheh , Pouryaghobi Seyyed Mohsen , Hashemnejad Maryam , Farahani Masoumeh , Rahimi Susan , Ataee Mina 
Published: February 2024

Objective:
One of the common methods of anaesthesia for caesarean sections (CSs) involves the use of spinal anaesthesia in mothers. Various positions are utilized in this method. This study aims to compare the evaluation of two positions, Trendelenburg and reverse Trendelenburg, in candidates for CS to assess the duration of anaesthesia and changes in vital signs in women.

Methods:
This study was a randomized clinical trial in which 60 pregnant mothers who met the inclusion criteria entered the study. These mothers were randomly allocated into two equal groups using block randomization. One group of patients received spinal anaesthesia in the Trendelenburg position, while the other group received it in the Reverse Trendelenburg position. Vital signs (systolic and diastolic blood pressure, heart rate, Apgar score, and SPO2) of participants from both groups were evaluated for 1 h after the induction of anaesthesia. Additionally, sensory level and duration of anaesthesia were measured. Finally, the data from both groups were subjected to statistical analysis using SPSS version 26 software.

Results:
The mean (SD) age of participating mothers in the Reverse Trendelenburg and Trendelenburg groups was 28.93 (5.82) and 30.97 (4.94), respectively. The two study groups did not significantly differ in baseline characteristics such as age, BMI, which could potentially impact vital sign outcomes or anaesthesia duration, and education (P>0.05). The mean (SD) duration of anaesthesia in the Trendelenburg position was significantly higher than in the Reverse Trendelenburg position [221.57(min) vs. 159.00(min)] (P<0.0001). There was no significant difference between the two positions, Trendelenburg and Reverse Trendelenburg, in terms of sensory level and its extent (P=0.08). The two study groups did not significantly differ in hemodynamic changes measured 13 times, including heart rate, systolic and diastolic blood pressure, and Apgar score (P>0.05).

Conclusion:
In spinal anaesthesia with the Trendelenburg position compared to the Reverse Trendelenburg position, there is a longer duration of anaesthesia. This is while the two positions did not differ in terms of hemodynamic changes and sensory level.
Brain N-acetyl-aspartyl-glutamate is associated with cognitive function in older virally suppressed people with HIV
Authors: Wiseman Robyn L. , Bigos Kristin L. , Dastgheyb Raha M. , Barker Peter B. , Rubin Leah H. , Slusher Barbara S. 
Published: February 2024

Objectives:Cognitive impairment persists in virally suppressed people with HIV (VS-PWH) especially in higher order domains. One cortical circuit, linked to these domains, is regulated byN-acetyl-aspartyl glutamate (NAAG), the endogenous agonist of the metabotropic glutamate receptor 3. The enzyme glutamate carboxypeptidase II (GCPII) catabolizes NAAG and is upregulated in aging and disease. Inhibition of GCPII increases brain NAAG and improves learning and memory in rodent and primate models.Design:As higher order cognitive impairment is present in VS-PWH, and NAAG has not been investigated in earlier magnetic resonance spectroscopy studies (MRS), we investigated if brain NAAG levels measured by MRS were associated with cognitive function.Methods:We conducted a retrospective analysis of 7-Tesla MRS data from a previously published study on cognition in older VS-PWH. The original study did not separately quantify NAAG, therefore, work for this report focused on relationships between regional NAAG levels in frontal white matter (FWM), left hippocampus, left basal ganglia and domain-specific cognitive performance in 40 VS-PWH after adjusting for confounds. Participants were older than 50 years, negative for affective and neurologic disorders, and had no prior 3-month psychoactive-substance use.Results:Higher NAAG levels in FWM were associated with better attention/working memory. Higher left basal ganglia NAAG related to better verbal fluency. There was a positive relationship between hippocampal NAAG and executive function which lost significance after correction for confounds.Conclusion:These data suggest brain NAAG serves as a biomarker of cognition in VS-PWH. Pharmacological modulation of brain NAAG warrants investigation as a therapeutic approach for cognitive deficits in VS-PWH.
Simplified immune-dysregulation index: A novel marker predicts 28-day mortality of intensive care patients with sepsis
Authors: Liu Shengjun , Zhuge Changjing , Zhang Jiahui , Cui Na , Long Yun 
Published: February 2024

AbstractBackgroundSepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. There is currently no simple immune-imbalance-driven indicator for patients with sepsis.MethodsThis study was conducted in Peking Union Medical College Hospital. Patients with Sepsis were identified according to Sepsis 3.0 after reviewing patient data from May 2018 through October 2022. LASSO logistic regression was used for features selection. Receiver operating characteristic curves for 28-day mortality were used to compare the predictive performance of level of interleukin-6 (IL-6) and lymphocyte count (LY#) with that of the combined ratio, namely, the IL-6/LY# ratio. A Cox hazard model was also employed to test the predictive performance of IL-6/LY# versus several other measurements. The dynamic trend of IL-6/LY# based on Day 1 IL-6/LY# level was analyzed.ResultsThe mortality rate was 24.5% (220/898) in the study cohort. The LY#, IL-6 level, blood platelet count, SOFA score, APACHE II score, heart rate, age and FiO2level were identified as key factors for predicting 28-day mortality. IL-6/LY# was identified as a core indicator according to LASSO logistic regression analysis. IL-6/LY# was significantly higher in nonsurvivors than in survivors (348 (154.6-1371.7) vs. 42.3 (15.4-117.1)). IL-6/LY# yielded a higher area under the curve (0.852 (95% CI 0.820–0.879)) than the level of IL-6 (0.776 (95% CI 0.738-0.809)) and LY# (0.719 (95% CI 0.677–0.755)) separately. Survival analysis of mortality risk versus the IL-6/LY# ratio suggested that IL-6/LY# was significantly more predictive of patient risk than the SOFA score or the other factors (p= 1.5 × 10-33). In trend analysis, as the trend of D1-D3-D7 IL-6/LY# decreases, the morality rate is lower than increase or fluctuate group (42.1% vs 58.3%, 37.9% vs 43.8%, 37.5% vs 38.5% in high, moderate and low D1 IL-6/LY# group separately).ConclusionsIL-6/LY# examined on first day in ICU can be used as an immune-imbalance alert to identifies sepsis patients with higher risk of 28-day mortality. Decreasing trend of IL-6/LY# suggest lower 28-day mortality rate of sepsis patients.
NEAR-INFRARED DYE IR-780 ALLEVIATES HEMATOPOIETIC SYSTEM DAMAGE BY PROMOTING HEMATOPOIETIC STEM CELLS INTO QUIESCENCE
Authors: Wu Jie , Ma Le , Gong Qiang , Chen Yan , Chen Long , Shi Chunmeng 
Published: February 2024

ABSTRACTPotential radiation exposure is a general concern, but there still lacks radioprotective countermeasures. Here, we found a small molecular near-infrared dye IR-780, which promoted hematopoietic stem cells (HSCs) into quiescence to resist stress. When mice were treated with IR-780 before stress, increased HSC quiescence and better hematopoietic recovery were observed in mice in stress conditions. However, when given after radiation, IR-780 did not show obvious benefit. Transplantation assay and colony-forming assay were carried out to determine self-renewal ability and repopulation capacity of HSCs. Furthermore, IR-780 pretreatment reduced the generation of reactive oxygen species (ROS) and DNA damage in HSCs after radiation. In homeostasis, the percentage of Lineage−, Sca-1+, and c-Kit+cells and long-term HSCs (LT-HSCs) were improved, and more HSCs were in G0 state after administration of IR-780. Further investigations showed that IR-780 selectively accumulated in mitochondria membrane potential high LT-HSCs (MMP-high LT-HSCs). Finally, IR-780 promoted human CD34+HSC reconstruction ability in NOD-PrkdcscidIl2rgnullmice after transplantation and improved repopulation capacityin vitroculture. Our research showed that IR-780 selectively entered MMP-high LT-HSCs and promoted them into dormancy, thus reducing hematopoietic injury and improving regeneration capacity. This novel approach might hold promise as a potential countermeasure for radiation injury.