bluuebunny/crossref_metadata_2025_split huggingface dataset:
Etiology of diarrheal hospitalizations following rotavirus vaccine implementation and association of enteric pathogens with malnutrition among under-five children in India
Authors: Varghese Tintu , Mills James A. Platts , Revathi R. , Antoni Sebastien , Soeters Heidi M. , Emmanuel Njambe Tondo Opute , Houpt Eric R. , Tate Jacqueline E. , Parashar Umesh D. , Kang Gagandeep 
Published: April 2024

AbstractMalnourished children are at higher risk of mortality and morbidity following diarrheal illness and certain enteropathogens have been associated with malnutrition in children. Very few studies have comprehensively looked at the etiology of diarrhea in malnourished children and most have used conventional diagnostic methods with suboptimal sensitivity. We used a highly sensitive molecular approach against a broad range of pathogens causing diarrhea and examined their association with malnutrition. In addition, we looked at the pathogen diversity of pediatric diarrhea, three years after the nationwide rotavirus vaccine introduction to understand the evolving landscape of pathogens, which is crucial for planning strategies to further reduce the diarrhea burden. Clinical details and diarrheal stool samples were collected from hospitalized children aged < 5 years from three sentinel sites in India for a period of one year. The samples were tested by qPCR for 16 established causes of diarrhea using TaqMan Array Cards. A total of 772 children were enrolled, from whom 482 (62.4%) stool specimens were tested. No specific pathogen was associated with diarrhea among children with acute or chronic malnutrition compared to those with better nutritional status. Overall, adenovirus was the leading pathogen (attributable fraction (AF) 16.9%; 95% CI 14.1 to 19.2) followed by rotavirus (AF 12.6%; 95% CI 11.8 to 13.1) and Shigella (AF 10.9%; 95% CI 8.4 to 16.4). The majority of diarrhea requiring hospitalization in children aged < 2 years could be attributed to viruses, while Shigella was the most common pathogen among children aged > 2 years. These data on the prevalence and epidemiology of enteropathogens identified potential pathogens for public health interventions.
Impact of amyloid and tau positivity on longitudinal brain atrophy in cognitively normal individuals
Authors: Fujishima Motonobu , Kawasaki Yohei , Mitsuhashi Toshiharu , Matsuda Hiroshi ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: April 2024

Abstract
Background
Individuals on the preclinical Alzheimer's continuum, particularly those with both amyloid and tau positivity (A + T +), display a rapid cognitive decline and elevated disease progression risk. However, limited studies exist on brain atrophy trajectories within this continuum over extended periods.

Methods
This study involved 367 ADNI participants grouped based on combinations of amyloid and tau statuses determined through cerebrospinal fluid tests. Using longitudinal MRI scans, brain atrophy was determined according to the whole brain, lateral ventricle, and hippocampal volumes and cortical thickness in AD-signature regions. Cognitive performance was evaluated with the Preclinical Alzheimer's Cognitive Composite (PACC). A generalized linear mixed-effects model was used to examine group × time interactions for these measures. In addition, progression risks to mild cognitive impairment (MCI) or dementia were compared among the groups using Cox proportional hazards models.

Results
A total of 367 participants (48 A + T + , 86 A + T − , 63 A − T + , and 170 A − T − ; mean age 73.8 years, mean follow-up 5.1 years, and 47.4% men) were included. For the lateral ventricle and PACC score, the A + T − and A + T + groups demonstrated statistically significantly greater volume expansion and cognitive decline over time than the A − T − group (lateral ventricle: β = 0.757 cm3/year [95% confidence interval 0.463 to 1.050], P < .001 for A + T − , and β = 0.889 cm3/year [0.523 to 1.255], P < .001 for A + T + ; PACC: β =  − 0.19 /year [− 0.36 to − 0.02], P = .029 for A + T − , and β =  − 0.59 /year [− 0.80 to − 0.37], P < .001 for A + T +). Notably, the A + T + group exhibited additional brain atrophy including the whole brain (β =  − 2.782 cm3/year [− 4.060 to − 1.504], P < .001), hippocampus (β =  − 0.057 cm3/year [− 0.085 to − 0.029], P < .001), and AD-signature regions (β =  − 0.02 mm/year [− 0.03 to − 0.01], P < .001). Cox proportional hazards models suggested an increased risk of progressing to MCI or dementia in the A + T + group versus the A − T − group (adjusted hazard ratio = 3.35 [1.76 to 6.39]).

Conclusions
In cognitively normal individuals, A + T + compounds brain atrophy and cognitive deterioration, amplifying the likelihood of disease progression. Therapeutic interventions targeting A + T + individuals could be pivotal in curbing brain atrophy, cognitive decline, and disease progression.
Surveillance of catheter-associated bloodstream infections: development and validation of a fully automated algorithm
Authors: Catho Gaud , Fortchantre Loïc , Teixeira Daniel , Galas-Haddad Murielle , Boroli Filippo , Chraïti Marie-Noëlle , Abbas Mohamed , Harbarth Stephan , Buetti Niccolò ,   Swissnoso group, Balmelli Carlo , Berthod Delphine , Jent Philipp , Marschall Jonas , Sax Hugo , Schlegel Matthias , Schweiger Alexander , Senn Laurence , Sommerstein Rami , Tschudin-Sutter Sarah , Troillet Nicolas , Vuichard-Gysin Danielle , Widmer Andreas F. , Wolfensberger Aline , Zingg Walter 
Published: April 2024

Abstract
Background
Most surveillance systems for catheter-related bloodstream infections (CRBSI) and central line-associated bloodstream infections (CLABSI) are based on manual chart review. Our objective was to validate a fully automated algorithm for CRBSI and CLABSI surveillance in intensive care units (ICU).

Methods
We developed a fully automated algorithm to detect CRBSI, CLABSI and ICU-onset bloodstream infections (ICU-BSI) in patients admitted to the ICU of a tertiary care hospital in Switzerland. The parameters included in the algorithm were based on a recently performed systematic review. Structured data on demographics, administrative data, central vascular catheter and microbiological results (blood cultures and other clinical cultures) obtained from the hospital’s data warehouse were processed by the algorithm. Validation for CRBSI was performed by comparing results with prospective manual BSI surveillance data over a 6-year period. CLABSI were retrospectively assessed over a 2-year period.

Results
From January 2016 to December 2021, 854 positive blood cultures were identified in 346 ICU patients. The median age was 61.7 years [IQR 50–70]; 205 (24%) positive samples were collected from female patients. The algorithm detected 5 CRBSI, 109 CLABSI and 280 ICU-BSI. The overall CRBSI and CLABSI incidence rates determined by automated surveillance for the period 2016 to 2021 were 0.18/1000 catheter-days (95% CI 0.06–0.41) and 3.86/1000 catheter days (95% CI: 3.17–4.65). The sensitivity, specificity, positive predictive and negative predictive values of the algorithm for CRBSI, were 83% (95% CI 43.7–96.9), 100% (95% CI 99.5–100), 100% (95% CI 56.5–100), and 99.9% (95% CI 99.2–100), respectively. One CRBSI was misclassified as an ICU-BSI by the algorithm because the same bacterium was identified in the blood culture and in a lower respiratory tract specimen. Manual review of CLABSI from January 2020 to December 2021 (n = 51) did not identify any errors in the algorithm.

Conclusions
A fully automated algorithm for CRBSI and CLABSI detection in critically-ill patients using only structured data provided valid results. The next step will be to assess the feasibility and external validity of implementing it in several hospitals with different electronic health record systems.
Stress, interpersonal and inter-role conflicts, and psychological health conditions among nurses: vicious and virtuous circles within and beyond the wards
Authors: Vallone Federica , Zurlo Maria Clelia 
Published: April 2024

Abstract
Background
The increasing costs of nurses’ occupational-stress, conflicts, and violence within healthcare services have raised international interest. Yet, research/interventions should consider that perceived stress and conflicts– but also potential resources– within the wards can crossover the healthcare settings, impacting nurses’ private lives and viceversa, potentially creating vicious circles exacerbating stress, conflicts/violence or, conversely, virtuous circles of psychological/relational wellbeing. Based on the Demands-Resources-and-Individual-Effects (DRIVE) Nurses Model, and responding to the need to go in-depth into this complex dynamic, this study aims to explore potential vicious circles featured by the negative effects of the interplay (main/mediating effects) between perceived stressors in nursing linked to interpersonal conflicts (Conflicts-with-Physicians, Peers, Supervisors, Patients/their families), work-family inter-role conflicts (Work-Family/Family-Work-Conflicts), and work-related stress (Effort-Reward-Imbalance) on nurses’ psychological/relational health (Anxiety, Depression, Somatization, Interpersonal-Sensitivity, Hostility). The potential moderating role of work-resources (Job-Control, Social-Support, Job-Satisfaction) in breaking vicious circles/promoting virtuous circles was also explored.

Method
The STROBE Checklist was used to report this cross-sectional multi-centre study. Overall, 265 nurses completed self-report questionnaires. Main/mediating/moderating hypotheses were tested by using Correlational-Analyses and Hayes-PROCESS-tool.

Results
Data confirmed the hypothesized detrimental vicious circles (main/mediating effects), impairing nurses’ psychological health conditions at individual level (Anxiety, Depression, Somatization), but also at relational level (Hostility and Interpersonal-Sensitivity). The moderating role of all work resources was fully supported.

Conclusion
Findings could be used to implement interventions/practices to effectively prevent the maintenance/exacerbation of vicious circles and promote psychological/relational wellbeing in healthcare settings and beyond.
B-CAT: a model for detecting botnet attacks using deep attack behavior analysis on network traffic flows
Authors: Putra Muhammad Aidiel Rachman , Ahmad Tohari , Hostiadi Dandy Pramana 
Published: April 2024

AbstractThreats on computer networks have been increasing rapidly, and irresponsible parties are always trying to exploit vulnerabilities in the network to do various dangerous things. One way to exploit vulnerabilities in a computer network is by employing malware. Botnets are a type of malware that infects and attacks targets in groups. Botnets develop quickly; the characteristics of initially sporadic attacks have grown into periodic and simultaneous. This rapid development has proved that the botnet is advanced and requires more attention and proper handling. Many studies have introduced detection models for botnet attack activity on computer networks. Apart from detecting the presence of botnet attacks, those studies have attempted to explore the characteristics of botnets, such as attack intensity, relationships between activities, and time segment analysis. However, there has been no research that explicitly detects those characteristics. On the other hand, each botnet characteristic requires different handling, while recognizing the characteristics of the botnet can help network administrators make appropriate decisions. Based on these reasons, this research builds a detection model that can recognize botnet characteristics using sequential traffic mining and similarity analysis. The proposed method consists of two main processes. The first is training to build a knowledge base, and the second is testing to detect botnet activity and attack characteristics. It involves dynamic thresholds to improve the model sensitivity in recognizing attack characteristics through similarity analysis. The novelty includes developing and combining analytical techniques of sequential traffic mining, similarity analysis, and dynamic threshold to detect and recognize the characteristics of botnet attacks explicitly on actual behavior in network traffic. Extensive experiments have been conducted for the evaluation using three different datasets whose results show better performance than others.
Improving medication prescribing-related outcomes for vulnerable elderly in transitions on high-risk medications (IMPROVE-IT HRM): a pilot randomized trial protocol
Authors: Holbrook Anne , Perri Dan , Levine Mitch , Mbuagbaw Lawrence , Jarmain Sarah , Thabane Lehana , Tarride Jean-Eric , Dolovich Lisa , Hyland Sylvia , Telford Victoria , Silva Jessyca , Nieuwstraten Carmine 
Published: April 2024

Abstract
Background
Seniors with recurrent hospitalizations who are taking multiple medications including high-risk medications are at particular risk for serious adverse medication events. We will assess whether an expert Clinical Pharmacology and Toxicology (CPT) medication management intervention during hospitalization with follow-up post-discharge and communication with circle of care is feasible and can decrease drug therapy problems amongst this group.

Methods
The design is a pragmatic pilot randomized trial with 1:1 patient-level concealed randomization with blinded outcome assessment and data analysis. Participants will be adults 65 years and older admitted to internal medicine services for more than 2 days, who have had at least one other hospitalization in the prior year, taking five or more chronic medications including at least one high-risk medication. The CPT intervention identifies medication targets; completes consult, including priorities for improving prescribing negotiated with the patient; starts the care plan; ensures a detailed discharge medication reconciliation and circle-of-care communication; and sees the patient at least twice after hospital discharge via virtual visits to consolidate the care plan in the community. Control group receives usual care. Primary outcomes are feasibility — recruitment, retention, costs, and clinical — number of drug therapy problems improved, with secondary outcomes examining coordination of transitions in care, quality of life, and healthcare utilization and costs. Follow-up is to 3-month posthospital discharge.

Discussion
If results support feasibility of ramp-up and promising clinical outcomes, a follow-up definitive trial will be organized using a developing national platform and medication appropriateness network. Since the intervention allows a very scarce medical specialty expertise to be offered via virtual care, there is potential to improve the safety, outcomes, and cost of care widely.

Trial registration number
ClinicalTrials.gov identifier: NCT04077281.
The diagnostic efficacy of diffusion tensor imaging in children with chronic kidney disease: correlation with histopathology and serum biomarkers
Authors: Mansour Manar , Elmokadem Ali H. , Elrazek Ahmed A. Abd , Hammad Ayman , Abd-Almoaty Marwa R. , Ali Khadiga M. , Ibrahim Dina Abdalla , Barakat Tarek Elsayed 
Published: April 2024

Abstract
Background
Children with chronic kidney disease (CKD) usually present with disease impact on growth besides cardiovascular problems that not only impact the patient's health during childhood but also affect their adult life. We aimed to identify the diagnostic role of diffusion tensor imaging (DTI) in CKD in pediatric using its metrics: apparent diffusion coefficient (ADC) and fraction anisotropy (FA).

Results
This prospective study was performed on thirty-five CKD patients (16 girls, 19 boys; mean age 12.3 ± 2.6 years) and 19 sex- and age-matched controls. Both groups underwent renal DTI and renal function tests. Based on renal biopsy, patients with CKD were further categorized into sclerotic CKD (n = 25) and non-sclerotic CKD (n = 10). Mean FA renal medulla/cortex in CKD (0.18 ± 0.18 and 0.20 ± 0.17) was lower significantly (p = 0.001) than volunteers' (0.31 ± 0.19, 0.27 ± 0.18). The cutoff FA of renal medulla/cortex used for CKD diagnosis was 0.22 and 0.23 with AUC of 0.828, 0.838 and accuracy of 80.8%, 82.8%. Mean of renal medulla/cortex ADC in CKD (2.13 ± 0.23 and 1.93 ± 0.22 × 10−3 mm2/s) was higher significantly (p = 0.001) than that of volunteers' (1.67 ± 0.15 and 1.64 ± 0.133 × 10−3 mm2/s. ADC cutoff value of renal medulla/cortex used for CKD diagnosis was 1.86 and 1.74 × 10−3 mm2/s with AUC of 0.827, 0.82, 0.827, and 0.911, and accuracy of 80.6%, 79.6%, 82.8%, and 84.2%. Renal medulla/cortex FA in sclerotic CKD was significantly different (p = 0.001) from non-sclerotic CKD (0.25 ± 0.07 and 0.26 ± 0.08). Cortical and medullary FA in CKD patients correlated with e-GFR (r = 0.363, r = 0.317) and serum creatinine (r = − 0.467, r = − 0.383).

Conclusions
Renal cortical/medullary FA can assist in diagnosing pediatric CKD, predict sclerotic CKD, and correlate with some serum biomarkers.
Brillouin microscopy monitors rapid responses in subcellular compartments
Authors: Coker Zachary N. , Troyanova-Wood Maria , Steelman Zachary A. , Ibey Bennett L. , Bixler Joel N. , Scully Marlan O. , Yakovlev Vladislav V. 
Published: April 2024

AbstractMeasurements and imaging of the mechanical response of biological cells are critical for understanding the mechanisms of many diseases, and for fundamental studies of energy, signal and force transduction. The recent emergence of Brillouin microscopy as a powerful non-contact, label-free way to non-invasively and non-destructively assess local viscoelastic properties provides an opportunity to expand the scope of biomechanical research to the sub-cellular level. Brillouin spectroscopy has recently been validated through static measurements of cell viscoelastic properties, however, fast (sub-second) measurements of sub-cellular cytomechanical changes have yet to be reported. In this report, we utilize a custom multimodal spectroscopy system to monitor for the very first time the rapid viscoelastic response of cells and subcellular structures to a short-duration electrical impulse. The cytomechanical response of three subcellular structures - cytoplasm, nucleoplasm, and nucleoli - were monitored, showing distinct mechanical changes despite an identical stimulus. Through this pioneering transformative study, we demonstrate the capability of Brillouin spectroscopy to measure rapid, real-time biomechanical changes within distinct subcellular compartments. Our results support the promising future of Brillouin spectroscopy within the broad scope of cellular biomechanics.
The exotic rust fungus
Puccinia abrupta
var.
partheniicola
on the invasive alien weed
Parthenium hysterophorus
in India: rediscovery and first report of an epiphytotic
Authors: Sreerama Kumar Prakya 
Published: April 2024

Abstract

Puccinia abrupta
var.
partheniicola
, the so-called winter rust of parthenium weed (
Parthenium hysterophorus
), an invasive alien weed in India, was rediscovered at an epiphytotic scale in February 2023 following a prolonged period of presumed absence in the field. Disease incidence was 80–90% in large tracts of
P. hysterophorus
stands along the roadsides in an area of 5 km
2
near Attibele–Sarjapura road (948A) in Anekal taluk of Bengaluru Urban district in Karnataka. Pycnial, aecial or telial stages of the rust were not found. The morphological parameters of uredinia and urediniospores completely matched those originally described, thus confirming the fungal identity. The significance of this rust epiphytotic to
P. hysterophorus
biocontrol in India is discussed together with the planned follow-up activities.
Numerical assessment of using FRP rebars as an alternative passive reinforcement in RC beams
Authors: Abdo Marwan , Abdelwahed Basem , El-Metwally Salah 
Published: April 2024

AbstractRecent sustainable building strategies make the right decisions to be environmentally friendly and reduce carbon emissions. In some reinforced concrete (RC) elements, fiber-reinforced polymer (FRP) bars have been proposed as an alternative to conventional steel bars. The demand for using noncorrosive and/or nonmetallic reinforcing bars in various reinforced concrete projects has increased. Although these concrete elements are lightweight, have a long lifespan, and need little maintenance, their non-ductile nature and bond with the surrounding concrete create significant challenges. Several experimental investigations have been conducted to evaluate the behavior of RC elements, even with their high cost. This study aims to assess numerically the viability of using FRP bars instead of traditional steel ones in simply supported reinforced concrete beams (RCBs) as longitudinal reinforcement (passive reinforcement). Utilizing the three-dimensional (3D) nonlinear finite element analysis (FEA) software (ABAQUS), a total of eighteen models were carried out to validate the results available in reference case studies with FRP bars. The verification of the numerical results has been verified by comparing them with the reference experimental data. Next, in order to assess the rigidity of such RCBs with FRP bars, parametric research was carried out. The numerical results proved that RCBs with FRP bars have a positive impact on enhancing load-carrying capacities. But on the other hand, the strain energy of such RCBs with CFRP bars is reduced to about 75% of the strain energy of RCBs with steel bars, which leads to low beam ductility.
Puberty Blocker, Leuprolide, Reduces Sex Differences in Rough-and-Tumble Play and Anxiety-like Behavior in Juvenile Rats
Authors: Oliveira Gabriela de Faria , Nguyen Amber T , Carreras-Simons Leykza , Niepsuj Thomas , Gadelhak Salma H , Johnson Aimee K , Abdalla Ashwakh , Lev Eden , Torres Román Sofia G , Fuchs Samantha N , Jorgensen Joan S , Farhat Walid A , Auger Anthony P 
Published: April 2024

Abstract
We examined the effect of the puberty blocker, leuprolide acetate, on sex differences in juvenile rough-and-tumble play behavior and anxiety-like behavior in adolescent male and female rats. We also evaluated leuprolide treatment on gonadal and pituitary hormone levels and activity-regulated cytoskeleton-protein messenger RNA levels within the adolescent amygdala, a region important both for rough-and-tumble play and anxiety-like behavior. Our findings suggest that leuprolide treatment lowered anxiety-like behavior during adolescent development, suggesting that the maturation of gonadotropin-releasing hormone systems may be linked to increased anxiety. These data provide a potential new model to understand the emergence of increased anxiety triggered around puberty. Leuprolide also reduced masculinized levels of rough-and-tumble play behavior, lowered follicle-stimulating hormone, and produced a consistent pattern of reducing or halting sex differences of hormone levels, including testosterone, growth hormone, thyrotropin, and corticosterone levels. Therefore, leuprolide treatment not only pauses sexual development of peripheral tissues, but also reduces sex differences in hormones, brain, and behavior, allowing for better harmonization of these systems following gender-affirming hormone treatment. These data contribute to the intended use of puberty blockers in stopping sex differences from developing further with the potential benefit of lowering anxiety-like behavior.