bluuebunny/crossref_metadata_2025_split huggingface dataset:
Implantation of scleral‐fixated posterior chamber intraocular lenses in adults
Authors: Kjeka Ole , Bohnstedt Jesper , Meberg Kyrre , Seland Johan H. 
Published: February 2008

Abstract.Purpose:  To report the results of transscleral fixation of posterior chamber intraocular lenses in adults.Methods:  We carried out a retrospective analysis of 91 eyes of 81 patients who underwent implantation of posterior chamber lenses with transscleral sutures between 1997 and 2006. The mean age of the patients was 62 years (range 19–94 years). Sixty‐eight eyes (74.7%) were aphakic at the time of surgery. In 10 patients (11.0%) an intracapsular cataract extraction and in six patients (6.6%) a pars plana lensectomy was performed prior to the fixation of the posterior chamber intraocular lens. In seven eyes (7.7%) a previously implanted IOL was removed. The mean follow‐up was 36 months (range 6–116 months).Results:  The mean preoperative best corrected visual acuity (BCVA) was 0.37 (range counting fingers to 1.0), which improved to 0.5 (range light perception to 1.0) postoperatively. At the end of follow‐up, BCVA was unchanged or improved in 81 eyes (89.0%), reduced by 2 Snellen lines in four eyes (4.4%), and between finger counting and light perception in four eyes (4.4%). The most serious complication was suprachoroidal haemorrhage, which occurred in two eyes. Retinal detachment occurred in three eyes, all of which successfully reattached after surgery. Suture erosion or spontaneous dislocation caused by suture degradation or breakage was not seen.Conclusions:  Secondary implantation of posterior chamber intraocular lenses with transscleral fixation is a reasonably safe procedure in adults, with relatively few serious complications. Even in patients with longterm follow‐up, suture breakage was not seen.
Allergic and photoallergic contact dermatitis from ketoprofen: results of (photo) patch testing and follow‐up of 42 patients*
Authors: Devleeschouwer Veerle , Roelandts Rik , Garmyn Marjan , Goossens An 
Published: February 2008

Background:  Photoallergic contact dermatitis from topical ketoprofen (KP), a nonsteroidal anti‐inflammatory agent, is a well‐known side effect.Objectives:  To investigate photo‐contact allergic reactions to KP and other nonsteroidal anti‐inflammatory drugs (NSAIDs), sunscreens, and fragrance components as well as the presence of prolonged photosensitivity related to it.Patients/Methods:  From June 1993 to June 2007, 42 patients were patch tested and photopatch tested with the ingredients of a KP preparation and other relevant substances. A questionnaire was performed in order to determine the importance of prolonged photosensitivity; 40/42 did respond.Results:  38 patients showed photo‐contact reaction, 1 photoaggravated reaction, and 3 contact allergic (CA) reaction to KP. Simultaneous photo‐contact allergic reactions were frequently observed not only to structurally related but also to non‐structurally related NSAIDs and sunscreens. Simultaneous CA to fragrance components was common. 1/3 of the patients reported prolonged photosensitivity, i.e. from 1 up to 14 years after having stopped KP application.Conclusions:  The history is often not a good guidance to determine KP‐related (photo) allergic contact dermatitis and the severe clinical symptoms sometimes require hospitalization, and/or systemic corticosteroids. As for the association between KP and sunscreen intolerance (being 1 of the possible causal factors for recurrent dermatitis), routine standard photopatch testing with KP might be indicated.
Burkitt lymphoma/leukaemia transformed from a precursor B cell: clinical and molecular aspects
Authors: Hassan Rocio , Felisbino Fabricio , Stefanoff Claudio Gustavo , Pires Virginia , Klumb Claudete E. , Dobbin Jane , Seuánez Héctor N. , Renault Ilana Zalcberg 
Published: February 2008

AbstractBurkitt lymphoma/leukaemia (BL/L) is a heterogeneous disease with respect to epidemiological patterns and cell origin. The occurrence of BL/L with an immature phenotype raises the question whether this phenotype might be a consequence of early B‐cell transformation or, alternatively, a secondary feature of transformed, mature B cells. It also poses important clinical questions regarding diagnosis and therapeutic procedures. Here we describe the case of a 4‐yr‐old child with BL/L and FAB L3 morphology, with phenotypic and genotypic characteristics of a CD10+ precursor B‐cell acute lymphoid leukaemia (ALL) associated with t(8;14)(q24;q32). Molecular analysis showed expression of RAG1 and RAG2 and an unmutated VDJCμ immunoglobulin rearrangement coinciding with a lack of AICDA expression, indicating an immature B‐cell origin. His clinical response suggested that FAB L3 ALL with MYC rearrangement and an aberrant precursor B‐cell phenotype is clinically similar to BL/L. Moreover, short, intensive chemotherapeutic protocols seemed to be beneficial. This case also allowed us to refine the description of cellular and molecular variants of BL/L regarding the cell origin and pathogenesis of this biologically heterogeneous disease.
Congenital dyserythropoietic anaemia, type I, in a Caucasian patient with retinal angioid streaks (homozygous arg1042trp mutation in codanin‐1)
Authors: Tamary H. , Offret H. , Dgany O. , Foliguet B. , Wickramasinghe S. N. , Krasnov T. , Rumilly F. , Goujard C. , Fénéant‐Thibault M. , Cynober T. , Delaunay J. 
Published: February 2008

AbstractA congenital dyserythropoietic anaemia (CDA) was recognised in a French Caucasian male patient. Blood smears showed a pronounced aniso‐poikilocytosis. Bone marrow light microscopy showed signs of dyserythropoesis, but no internuclear chromatin bridges. Electron microscopy disclosed erythroblast nuclei with the Swiss cheese aspect and the presence of cytoplasmic organelles, assessing the diagnosis of CDA I. The presence of internuclear chromatin bridges may thus be missing in CDA I. The patient proved to be homozygous for the Arg1042Trp mutation in codanin‐1 (the ‘Bedouin mutation’). By the age of 25, the patient’s vision started to deteriorate as a result of retinal angioid streaks and macular abnormalities. Evolution was controlled and the patient, being nearly 50 yr old now, still has a partial use of his eyes. This second case of retinal angioid streaks reported in CDA I adds to the non‐haematological features likely to be associated with this condition.
Genomic tissue typing and optimal antithymocyte globuline dose using unrelated donors results in similar survival and relapse as HLA‐identical siblings in haematopoietic stem‐cell transplantation for leukaemia
Authors: Remberger Mats , Mattsson Jonas , Hausenberger Dan , Schaffer Marie , Svahn Britt‐Marie , Ringdén Olle 
Published: February 2008

AbstractSixty‐one leukaemia patients treated with haematopoietic stem cell transplantation (HSCT) from a genomic human leucocyte antigen (HLA)‐A, ‐B and –DRβ1 matched unrelated donor (MUD) were compared with 121 patients with an HLA‐identical sibling donor. All patients received conventional conditioning. We selected all patients with unrelated donors who received optimal antithymocyte globuline (ATG) dose, 6 mg/kg. One hundred and seven patients received stem cells from peripheral blood and 75 patients received bone marrow (BM) cells. The incidences of acute graft‐versus‐host disease (GVHD) grades II–IV were 33.4% and 34.7% in the MUD and sibling group, respectively. After year 2001, the incidence of chronic GVHD was similar in the two groups (27.8% vs. 25.8%). There was no difference in overall survival (60% vs. 60%), transplant‐related mortality (18.6% vs. 16.6%) and relapse (23% vs. 26.4%) between the two groups. Conclusion: Haematopoietic stem cell transplantation with unrelated donors results in similar GVHD, relapse and survival as compared to using sibling donors. Reasons for this may be improved tissue‐typing techniques and supportive care and optimisation of the ATG dose.
ORIGINAL ARTICLE: Serial Estimation of Th1:Th2 Cytokines Profile in Women Undergoing In‐Vitro Fertilization‐Embryo Transfer
Authors: Kalu Emmanuel , Bhaskaran Shree , Thum Meen Yau , Vishwanatha Radhika , Croucher Carolyn , Sherriff Elizabeth , Ford Brian , Bansal Amolak S. 
Published: February 2008

ProblemTo investigate changes in the ratio of T‐cell subpopulations expressing intracellular T helper1 (Th1) and T helper 2 (Th2) cytokines in women with a history of recurrent failed implantation under going in‐vitro fertilization (IVF)‐embryo transfer.Method of studyTwenty‐eight peripheral blood samples were obtained at two time points, from 14 women undergoing IVF treatment; eight women with a history of recurrent failed implantation, who did not get pregnant in the index IVF cycle and six who had one or more previous successful IVF pregnancy and who became pregnant in the index IVF cycle. The proportion of lymphocytes expressing interferon‐gamma (IFN‐γ), tumour necrosis factor‐alpha (TNF‐α), and interleukin 4 (IL‐4) and the Th1:Th2 ratios of IFN‐γ:IL‐4, and TNF‐α:IL‐4 in T helper cells was measured by flow cytometry, in samples obtained before commencing IVF treatment and in samples obtained after ovarian stimulation (on the day of oocyte retrieval).ResultsIn samples collected during oocyte retrieval, women with a history of recurrent failed implantation had a higher IFN‐γ:IL‐4 and TNF‐α:IL‐4 ratio than the control group, (18.6 ± 9.3 versus 6.47 ± 1.68, P = 0.009) and (39.1 ± 15.7 versus 11.53 ± 3.76, P = 0.001) respectively. In women with a history of recurrent failed implantation the ratio of IFN‐γ:IL‐4 and TNF‐α:IL‐4 at oocyte retrieval was higher than pre‐treatment ratios (18.6 ± 9.3 versus 12.01 ± 9.8, P = 0.018) and 39.10 ± 15.7 versus 18.66 ± 11.42, P = 0.010) respectively, showing a Th1 bias. In women with a successful IVF the converse was true; the ratio at oocyte retrieval was significantly lower than pre‐treatment ratios (6.47 ± 1.68 versus 9.37 ± 6.8, P = 0.035) and 11.53 ± 3.76 versus 18.60 ± 12.9, P = 0.027) respectively, representing a Th2 bias.ConclusionWomen with a history of unexplained recurrent failed IVF treatment have a Th1 bias and this polarization is more enhanced following hormonal manipulations during IVF treatment. Comparing pre‐treatment ratios of IFN‐γ:IL‐4 and TNF‐α:IL‐4 to ratios obtained at oocyte retrieval may be clinically useful. Women with recurrent failed IVF have increasing ratios.
ORIGINAL ARTICLE: Investigation of Recombinant Mouse Sperm Protein Izumo as a Potential Immunocontraceptive Antigen
Authors: Wang De‐Gang , Huang Tian‐Hua , Xie Qing‐Dong , An Gang 
Published: February 2008

ProblemTo determine if the recombinant mouse Izumo (mIzumo) could be used as a potential immunocontraceptive antigen.Method of studyThe recombinant mIzumo fused with 6His tag (6His‐mIzumo) was purified by immobilized Ni2+ affinity chromatography. Enzyme‐linked immunosorbent assay and Western blot were used to detect anti‐6His‐mIzumo activities of serum from the mice immunized with 6His‐mIzumo. Inhibition of the anti‐6His‐mIzumo antibody on mouse sperm–egg fusion in vitro was performed using the zona free oocytes and acrosome reacted sperm. Fertility of the 6His‐mIzumo immunized male and female mice was compared with control mice.ResultsThe recombinant mIzumo was successfully produced. Female and male mice inoculated with 6His‐mIzumo developed a specific serum antibody and the highest antibody titer lasted at least 6 weeks. The serum anti‐6His‐mIzumo antibody almost completely blocked mouse sperm–egg fusion in vitro. However, there was no significant reduction in fertility for both male and female mice immunized with 6His‐mIzumo compared with control mice.ConclusionThe circulated anti‐mIzumo antibody can block mouse sperm–egg fusion in vitro but has no effect on fertility in vivo. It seems that application of Izumo as a candidate antigen in development of contraceptive vaccine needs further investigation.
ORIGINAL ARTICLE: Prednisolone Suppresses NK Cell Cytotoxicity In Vitro in Women With a History of Infertility and Elevated NK Cell Cytotoxicity
Authors: Thum Meen‐Yau , Bhaskaran Shree , Abdalla Hossam I , Ford Brian , Sumar Nazira , Bansal Amolak 
Published: February 2008

ProblemTo evaluate the effect of prednisolone on NK cell cytotoxicity in vitro environment and also to compare the effect of prednisolone versus immunoglobulin‐G (IVIG) on NK cell cytotoxicity using in vitro co‐culture with K562 cells.Method of studyThe following is a prospective observational study, between August 2006 and February 2007, was carried out on blood samples from 110 patients with a history of recurrent miscarriage or recurrent failed implantation. Peripheral blood mononuclear cells containing NK cells were isolated and co‐cultured with target cell K562 in three different effector‐to‐target (E:T) ratios of 50:1, 25:1 and 12.5:1. Prednisolone or IVIG was then added to the tube with E:T ratio of 50:1 to assess suppressive effect. The percentage killing was recorded and statistical analysis performed using Student’s t–test.ResultsIn the experiments with an E:T ratio of 50:1 without prednisolone or IVIG in the co‐culture, the mean target cell killing percentage was 26.4%. In cultures using the same E:T ratio, this killing percentage was significantly reduced in the presence of IVIG (9.9%) or prednisolone (13.6%), (P < 0.001 in both analyses). On comparing the reduction in killing percentage of target cells by prednisolone versus IVIG, a slightly lower reduction in the prednisolone co‐culture was noted but this was not statistically significant (P > 0.05).ConclusionThe results of this study show that prednisolone is able to suppress the cytolytic activity of the NK cell. Prednisolone and IVIG are almost equally effective in suppressing in vitro NK cell cytolytic activity.
Non‐surgical periodontal treatment of cyclosporin A‐induced gingival overgrowth: immunohistochemical results
Authors: Aimetti M , Romano F , Marsico A , Navone R 
Published: February 2008

Aim:  The present study was planned to analyze the effects of a 12‐month non‐surgical periodontal treatment on histologic and immunohistochemical features of cyclosporin A (CsA)‐induced gingival overgrowth (GO).Materials and methods:  Gingival samples were collected from 21 liver transplant subjects exhibiting CsA‐induced GO prior to, and 12 months after non‐surgical periodontal therapy including oral hygiene instructions, scaling and 2‐month recall appointments, and also from 18 healthy control subjects. Gingival biopsy specimens were stained with hematoxylin–eosin and monoclonal antibodies for vimentin, CD3 (T‐lymphocytes), CD20 (B‐lymphocytes), CD34 (endothelium) and Ki‐67 (fibroblasts proliferation rate), using a streptavidin‐biotin‐peroxidase complex method.Results:  Total inflammatory cells, gingival vessels and fibroblast proliferation rate demonstrated significant reduction after non‐surgical periodontal treatment (P < 0.0001) in overgrown gingiva, while B‐ and T‐lymphocytes remained nearly unchanged (P = 0.61 and 0.33, respectively). At the 12‐month evaluation no significant differences were found when comparing the gingival biopsies from CsA‐treated patients and those from healthy controls (P > 0.05).Conclusions:  Control of clinical inflammation by means of non‐surgical periodontal treatment results both in lowering of inflammatory infiltrate and in changes in connective tissue composition. Thus, plaque‐induced inflammation would seem to modulate the drug‐gingival tissue interaction.Clinical relevance:  A strict plaque control program play a pivotal role in the management of transplant patients exhibiting cyclosporin A‐GO.
α‐Lipoic acid treatment of 31 patients with sore, burning mouth
Authors: Steele JC , Bruce AJ , Drage LA , Rogers RS 
Published: February 2008

Objective:  To review a series of patients with sore, burning mouth treated with α‐lipoic acid between 2000 and May 2006 and subjectively evaluate improvement in symptoms.Design:  Retrospective review of medical records of 195 consecutive patients who sought treatment for sore, burning mouth. Treatment of 47 patients was a prescription/recommendation for α‐lipoic acid. Of these patients, 35 were available for follow‐up.Setting:  Tertiary care academic medical center.Subjects:  Ambulatory patients given prescription /recommendation for α‐lipoic acid 600 mg per day, in divided doses.Main Outcome Measure:  Reported improvement in symptoms documented in medical records and at follow‐up (visits or telephone interviews).Results:  Thirty‐one of the 35 patients (66% of all 47) actually took α‐lipoic acid as recommended. No patient reported a complete alleviation of symptoms. Six (19%) of these 31 patients felt mostly better, five (16%) felt somewhat better, and 14 (45%) reported no difference. Two patients (7%) reported a worsening of symptoms and four (13%) did not know whether there had been improvement.Conclusion:  Eleven of 31 patients (35%) reported benefit from taking α‐lipoic acid. Because we examined only a small number of patients and relied on a subjective outcome assessment, further larger studies using a prospective, randomized, controlled, and double‐blind structure are warranted.
Obesity – a social and physical risk
Authors: Parlesak Alexandr , Krömker Dörthe 
Published: February 2008

SummaryOverweight and obesity are an accepted cause of numerous metabolic disor‐ders. The obvious strategies for prevention and therapy of increased fat body mass (reduction of energy intake, increase of physical activity) fail in most cases, especially with respect to their sustainability. The lack of success of programs developed so far for prevention and therapy of obesity suggests the existence of poorly‐understood regulatory mechanisms leading to the imbalance between energy uptake and energy expenditure. Besides physiological factors,such as dysregulated levels of hunger‐controlling or satiation‐mediating hormones, the composition of the intestinal flora and synthetic compounds with hormone‐like activity have been suggested as triggers of the development of obesity.Along with biological and metabolic parameters, scientists have focused more and more on psychological and sociological factors in the development of a strategy for the prevention of obesity. Important factors in this context are the motivation and ability to self‐regulation and aspects of the socio‐cultural context. Consequently, the real challenge in reducing the prevalence of obesity is not only the identification of relevant parameters but also the assessment of proportionality of these factors. To achieve this goal, borders of disciplines dealing with obesity development (nutrition, medicine, kinematics, psychology, sociology) need to be overcome. Common models must be developed that facilitate the assessment of both the importance of single factors and their relationship to each other.
Mapping Nursing Interventions From a Hospital Information System to the Nursing Interventions Classification (NIC)
Authors: Coenen Amy , Ryan Polly , Sutton Janice 
Published: February 2008

TOPIC. Using the Nursing Interventions Classifications (NIC) as a standard to classify interventions identified in a hospital intervention system. SOURCES. Literature and the authors experience with standardized data systems for nursing. PURPOSE. To describe the processes used and the issues that arose as interventions from a hospital nursing information system were mapped to interventions in the NIC. CONCLUSIONS. The NIC provided a standard for the classification of nursing interventions from a hospital information system resulting in data that could be compared across sites. Ongoing testing of nursing intervention classifications is recommended.
Comparison of the abilities of centipedegrass (Eremochloa ophiuroides Hack.) and zoysiagrass (Zoysia japonica Steud.) to purify liquid animal waste
Authors: Takada‐Oikawa Naoko , Kawanabe Sukeo , Oshida Toshio , Ukiya Yuya 
Published: February 2008

AbstractIn the land application system to purify liquid animal waste, the introduction of vegetation has further increased treatment ability. Although the vegetation beds were covered with zoysiagrass (Zoysia japonica Steud.), we introduced centipedegrass (Eremochloa ophiuroides Hack.) into an experimental bed in order to promote the purification activity of secondary treatment after primary treatment during a midsummer period because centipedegrass thrives even in hotter conditions and to compare its purification ability with that of zoysiagrass. The results showed that the centipedegrass bed had a higher purification ability than zoysiagrass beds in a hot season. The drainage material purified through the centipedegrass bed demonstrated lower values of turbidity, electric conductivity, chemical oxygen demand, total phosphorus, total nitrogen and ammonium‐nitrogen, and higher concentrations of nitrate‐nitrogen than that purified through the zoysiagrass bed during the middle vegetative stage when the centipedegrass reached the highest biomass. These results suggested that nitrification had occurred vigorously in the centipedegrass plot. It was suggested that the higher nitrogen purification ability of the centipedegrass was linked to both better absorption of nitrogen and nitrification.
Seasonal changes in herbage production and soil phosphorus contents in Japanese lawngrass (Zoysia japonica Steud.) and tall fescue (Festuca arundinacea Schreb.) pastures
Authors: Kaneko Makoto , Kurokawa Yuzo , Tanaka Haruo , Suzuki Sohzoh , Itabashi Hisao 
Published: February 2008

AbstractSeasonal changes in the above‐ground phosphorus (P), soil total P (TP), soil Olsen P (OP) and soil microbial biomass P (MBP) were investigated for 2 years in Japanese lawngrass (Zy) and tall fescue (Tf) pastures on Japanese Andosol, with the goal of clarifying P characteristics in the Zy pasture in comparison with the Tf pasture. The soil P attributes were measured in two soil layers (root mat layer, 0–2.5 cm depth; under layer, 5–10 cm depth). The P concentration of the above‐ground herbage in the Zy pasture, which was higher than the standard value and similar to those in the Tf pasture, might have contributed to the large amounts of the above‐ground P mass. The lack of plowing management and the coverage with Japanese lawngrass might have changed soil TP. The TP, the OP and the OP/TP in the Zy pasture were higher than those in the Tf pasture, and the TP, the OP and the OP/TP at the root mat layer were higher than those at the under layer. A large amount of the TP and high P availability in the soil caused the large amounts of OP. Soil pH, soil microorganisms and MBP might have affected soil P availability in the Zy pasture. Plant litter in the root mat layer of the Zy pasture may have increased soil P accumulation and its availability, which might be reasons for the high P uptake in the present study. Japanese lawngrass pasture may be a system with improved soil P utilization efficiency based on P cycling.