bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microsatellite instability, MLH1 promoter methylation, and BRAF mutation analysis in sporadic colorectal cancers of different ethnic groups in Israel
Authors: Vilkin Alex , Niv Yaron , Nagasaka Takeshi , Morgenstern Sarah , Levi Zohar , Fireman Zvi , Fuerst Florentine , Goel Ajay , Boland C. Richard 
Published: February 2009

AbstractBACKGROUND:The molecular mechanisms that underlie colorectal cancer (CRC) include microsatellite instability (MSI), chromosomal instability, and the CpG island methylator phenotype. There is evidence to suggest that CRC incidence varies among different ethnic populations worldwide. The authors of this report hypothesized that environmental factors and lifestyle differences among various ethnic groups may differentially influence the epigenetic regulation of tumor suppressor genes in CRC.METHODS:In the current study, microdissection and DNA extraction were performed on 128 samples of CRC from Israeli patients (85 Jews and 43 Arabs). MSI analysis, mutL homolog 1 (MLH1) and mutS homolog 2 (MSH2) protein expression levels, and MLH1 promoter methylation were investigated by combined bisulfite restriction analysis. The v‐raf murine sarcoma viral oncogene homolog B1 (BRAF) valine‐to‐glutamic acid mutation at residue 600 was investigated by direct DNA sequencing.RESULTS:High MSI (MSI‐H), MLH1 methylation, and BRAF mutations were observed in 11.6%, 9.4%, and 23.5% of Jews, respectively, and in 16.2%, 17.6%, and 20.9% of Arabs, respectively (P value nonsignificant). MLH1 promoter methylation was observed in 22.6% of microsatellite‐stable (MSS) tumors and in 53.8% of MSI‐H tumors (P < .015). Extensive methylation (covering both 5′ and 3′ promoter regions) was present in all MSI‐H tumors with loss of MLH1 expression. BRAF mutation was observed in 15.6% and 46.1% of MSS tumors and MSI‐H tumors, respectively (P < .007). BRAF mutation was observed in 66%, 22.2%, and 14.7% of patients who had tumors with extensive MLH1 promoter methylation, methylation of the 5′ region alone, or without methylation, respectively (P < .006).CONCLUSIONS:There was no difference in molecular signatures examined between Jewish and Arab patients with CRC in Israel. Extensive promoter methylation was associated with MLH1 inactivation, MSI, and BRAF mutation. Cancer 2009. © 2009 American Cancer Society.
Association of progression‐free survival, overall survival, and patient‐reported outcomes by skin toxicity and KRAS status in patients receiving panitumumab monotherapy
Authors: Peeters Marc , Siena Salvatore , Van Cutsem Eric , Sobrero Alberto , Hendlisz Alain , Cascinu Stefano , Kalofonos Haralabos , Devercelli Giovanna , Wolf Michael , Amado Rafael G. 
Published: February 2009

AbstractBACKGROUND:The authors explored the association of skin toxicity (ST) severity as measured by patient‐reported ST and Common Terminology Criteria for Adverse Events (CTCAE) grading with efficacy of panitumumab, a fully human antiepidermal growth factor receptor antibody, from a phase 3 metastatic colorectal cancer (CRC) trial.METHODS:Patients were randomized to panitumumab plus best supportive care (BSC) vs BSC alone. ST by modified National Cancer Institute CTCAE v3.0 and modified Dermatology Life Quality Index (mDLQI), health‐related quality of life (HRQOL), and CRC symptoms were measured. ST was analyzed using a landmark approach. Associations by KRAS mutational status were also assessed.RESULTS:Of 463 patients, 208 of 231 (90%) panitumumab patients and 184 of 232 (79%) BSC patients had ≥1 postbaseline patient‐reported outcome (PRO) assessment. Panitumumab patients with more severe ST had significantly longer overall survival (OS) (grade 2‐4:grade 1; hazard ratio, 0.60; P = .0033). Lower mDLQI scores (<67; more bothersome ST) were associated with longer OS (Cox model, P < .0001). Similar results were observed with progression‐free survival (PFS). An inverse relation between mDLQI and HRQOL scores was observed, suggesting that ST bother correlated with better HRQOL. KRAS and PRO data were available in 363 patients (188 panitumumab; 175 BSC). Longer OS was associated with lower mDLQI scores, regardless of KRAS status. Longer PFS was associated with more severe ST (lower mDLQI scores and higher CTCAE grade ST) in patients with wild‐type (WT) KRAS tumors, but not in patients with mutant KRAS tumors.CONCLUSIONS:More severe ST, by both clinical grading and PRO, is associated with better CRC symptoms and HRQOL and with longer OS and PFS among panitumumab‐treated patients. The associations for PFS were more pronounced in patients with WT KRAS tumors. Cancer 2009. © 2009 American Cancer Society.
Invader human papillomavirus (HPV) type 16 and 18 assays as adjuncts to HPV screening of cervical papanicolaou smears with atypical squamous cells of undetermined significance
Authors: Wong Anna K. , Chan Raymond C. , Nichols W. Stephen , Bose Shikha 
Published: February 2009

AbstractBACKGROUND:High‐risk (HR) human papillomavirus (HPV) testing is standard practice for triaging women who have Papanicolaou (Pap) smears with atypical squamous cells of undetermined significance (ASC‐US), however, only 5% to 17% of these women have underlying cervical intraepithelial neoplasia 2 (CIN‐2)/CIN‐3. Recent reports have demonstrated that the presence of either HPV type 16 (HPV‐16) or HPV‐18 confers an elevated risk for CIN‐2/CIN‐3. The current study was designed to determine the prevalence of HPV‐16 and HPV‐18 in ASC‐US Pap smears and to determine whether further typing would enhance the risk stratification of patients for CIN‐2/CIN‐3.METHODS:One hundred seventy‐eight Pap smears with ASC‐US were screened retrospectively for HR HPV by using the proprietary Invader screening assay followed by typing for HPV‐16 and HPV‐18 by using Invader type‐specific probes on 100 of the samples. Clinical follow‐up results were correlated with HPV types.RESULTS:Fifty‐one percent of the ASC‐US samples were positive for HR HPV, the majority of which (70%) harbored non‐HPV‐16/HPV‐18 HR HPV types; 27% were associated with HPV‐16, whereas only 3% contained HPV‐18. The screening assay indicated that 46% of women who had Pap smears with ASC‐US were in need of further HPV‐16/HPV‐18 typing. Testing for HPV‐16 stratified women with ASC‐US into 3 groups: 1) 14% of women were positive for HPV‐16 and had a high risk (54%) of CIN‐2/CIN‐3 on follow‐up biopsy, 2) 35% of women were positive for non‐HPV‐16 HPV types and had an intermediate risk (9%), and 3) 51% of women were negative for HPV and had a negligible risk for CIN‐2/CIN‐3.CONCLUSIONS:The combined application of a proprietary screening assay and a type‐specific HPV‐16 assay demonstrated global potential for the development of tailored management protocols for women who have Pap smears with ASC‐US. Cancer 2009. © 2009 American Cancer Society.
Accuracy of the BRCAPRO model among women with bilateral breast cancer
Authors: Ready Kaylene J. , Vogel Kristen J. , Atchley Deann P. , Broglio Kristine R. , Solomon Kimberly K. , Amos Christopher , Lu Karen H. , Hortobagyi Gabriel N. , Arun Banu 
Published: February 2009

AbstractBACKGROUND:The likelihood of identifying a BRCA mutation was often calculated using the BRCAPRO model. A previous study suggested that this model may overestimate the chance of detecting a BRCA mutation among women diagnosed with bilateral breast cancer. Studies also suggested that few patients with bilateral breast cancer whose age at first diagnosis is >40 years were mutation carriers. The objectives of this study were to determine the accuracy of the BRCAPRO model among women with bilateral breast cancer and to determine whether their mutation status was dependent on their age at first diagnosis.METHODS:A retrospective chart review was performed. Women who were diagnosed with bilateral or unilateral breast cancer and who had undergone comprehensive BRCA1 and BRCA2 genetic testing at M. D. Anderson Cancer Center between 1997 and 2006 were included in the study.RESULTS:For individuals with pre‐test carrier probabilities >31%, the proportion of positive tests was significantly lower than predicted by the BRCAPRO model (P < .05). In addition, the carrier rate of BRCA mutations was significantly higher (P = .002, Fisher exact test) in women with bilateral breast cancer whose age at first diagnosis was ≤40 years compared with those diagnosed >40 years.CONCLUSIONS:The BRCAPRO model was overestimating the relative contribution bilateral breast cancer had on the likelihood of detecting a BRCA1 or BRCA2 mutation. Bilateral breast cancer did not appear to be a good indicator of mutation status, particularly for women whose age at first diagnosis is >40 years. Cancer 2009. © 2009 American Cancer Society.
Factors related to the presentation of thin and thick nodular melanoma from a population‐based cancer registry in Queensland Australia
Authors: Geller Alan C. , Elwood Mark , Swetter Susan M. , Brooks Daniel R. , Aitken Joanne , Youl Philippa H. , Demierre Marie‐France , Baade Peter D. 
Published: February 2009

AbstractBACKGROUND:Worldwide, the incidence of thick melanoma has not declined, and the nodular melanoma (NM) subtype accounts for nearly 40% of newly diagnosed thick melanoma. To assess differences between patients with thin (≤2.00 mm) and thick (≥2.01 mm) nodular melanoma, the authors evaluated factors such as demographics, melanoma detection patterns, tumor visibility, and physician screening for NM alone and compared clinical presentation and anatomic location of NM with superficial spreading melanoma (SSM).METHODS:The authors used data from a large population‐based study of Queensland (Australia) residents diagnosed with melanoma. Queensland residents aged 20 to 75 years with histologically confirmed first primary invasive cutaneous melanoma were eligible for the study, and all questionnaires were conducted by telephone (response rate, 77.9%).RESULTS:During this 4‐year period, 369 patients with nodular melanoma were interviewed, of whom 56.7% were diagnosed with tumors ≤2.00 mm. Men, older individuals, and those who had not been screened by a physician in the past 3 years were more likely to have nodular tumors of greater thickness. Thickest nodular melanoma (4 mm+) was also most common in persons who had not been screened by a physician within the past 3 years (odds ratio, 3.75; 95% confidence interval, 1.47‐9.59). Forty‐six percent of patients with thin nodular melanoma (≤2.00 mm) reported a change in color, compared with 64% of patients with thin SSM and 26% of patients with thick nodular melanoma (>2.00 mm).CONCLUSIONS:Awareness of factors related to earlier detection of potentially fatal nodular melanomas, including the benefits of a physician examination, should be useful in enhancing public and professional education strategies. Particular awareness of clinical warning signs associated with thin nodular melanoma should allow for more prompt diagnosis and treatment of this subtype. Cancer 2009. © 2009 American Cancer Society.
Is It Possible to Dope Single‐Walled Carbon Nanotubes and Graphene with Sulfur?
Authors: Denis Pablo A. , Faccio Ricardo , Mombru Alvaro W. 
Published: February 2009

AbstractSulfur doping of single‐walled carbon nanotubes and graphene is possible from a thermodynamic standpoint, according to DFT calculations. In the case of graphene, the doped sheet can be a small‐band‐gap semiconductor, as revealed by a plot of the band structure (see picture), or it can have better metallic properties than the pristine sheet, depending on the level of S doping.magnified imageHerein, we investigate sulfur substitutional defects in single‐walled carbon nanotubes (SWCNTs) and graphene by using first‐principles calculations. The estimated formation energies for the (3,3), (5,5), and (10,0) SWCNTs and graphene lie between 0.9 and 3.8 eV, at sulfur concentrations of 1.7–4 atom %. Thus, from a thermodynamic standpoint, sulfur doping is not difficult. Indeed, these values can be compared with that of 0.7 eV obtained for a nitrogen‐doped (5,5) SWCNT. We suggest that it may be possible to introduce sulfur into the SWCNT framework by employing sulfur‐containing heterocycles. Our simulations indicate that sulfur doping can modify the electronic structure of the SWCNTs and graphene, depending on the sulfur content. In the case of graphene, sulfur doping can induce different effects: the doped sheet can be a small‐band‐gap semiconductor, or it can have better metallic properties than the pristine sheet. Thus, S‐doped graphene may be a smart choice for constructing nanoelectronic devices, since it is possible to modulate the electronic properties of the sheet by adjusting the amount of sulfur introduced. Different synthetic routes to produce sulfur‐doped graphene are discussed.
Differential Sensing of Serine and Tyrosine with Aligned CdS Nanowire Arrays Based on pH‐Dependent Photoluminescence Behavior
Authors: Lin Yi‐Feng , Hsu Yung‐Jung , Cheng Wei‐Yun , Lu Shih‐Yuan 
Published: February 2009

AbstractUp and down: Differential sensing of serine and tyrosine is achieved with aligned CdS nanowire arrays by exploring the pH‐dependent PL behavior of the nanowire arrays toward exposure to the two amino acid solutions (see picture).magnified imageThe differential sensing of tyrosine and serine is achieved with well‐aligned CdS nanowire arrays by exploring the pH‐dependent photoluminescence behavior of the nanowire arrays toward exposure to the two amino acid solutions. The contrasting trend in photoluminescence (PL) intensity with respect to variations in analyte concentration observed at pH 11 served as the check point for the present differential sensing. The application format of the nanowire array is better suited for further sensing device assembly than that of nanocrystal suspensions.
Bericht über die 68. Sitzung des Ständigen Ausschusses für das Bauwesen
Authors: Springborn Matthias 
Published: February 2009

AbstractAm 10. und 11. November 2008 fand die 68. Sitzung des Ständigen Ausschusses für das Bauwesen (StAB) in Brüssel statt. Den Vorsitz hatte der Leiter des Referats “Baugewerbe” bei den Kommissionsdiensten.Im Folgenden werden die wesentlichen Beratungsinhalte kurz wiedergegeben.1. Berichte über die Ergebnisse verschiedener europäischer Arbeitsgruppen2. Harmonisierte Normen3. Änderung zweier Mandate an EOTA4. Kommissionsentscheidungen5. Mandate6. Entwurf einer Verordnung über Bauprodukte7. Verordnung über gegenseitige Anerkennung8. Sonstiges
Erläuterung der Regelung von dämmschichtbildenden Baustoffen
Authors: Dierke Sabine 
Published: February 2009

Abstract1. Zulassungsgegenstand2. Zulassungsgrundsätze3. Zulassungsverfahren4. Allgemeine bauaufsichtliche Zulassung für dämmschichtbildende Baustoffe5. Änderungen am Bauprodukt “Dämmschichtbildender Baustoff”6. Fremdüberwachung7. Eigenüberwachung – Werkseigene Produktionskontrolle
Bericht über die DIBt‐Tagung “Dübel und Verankerungen – Planung und Anwendungen”
Authors: Feistel Georg 
Published: February 2009

AbstractAm 21. November 2008 veranstaltete das Deutsche Institut für Bautechnik (DIBt) eine Tagung zum Thema Dübel und Verankerungen – Planung und Anwendungen. Sie fand im Rahmen einer Veranstaltungsreihe zum 40‐jährigen Jubiläum des DIBt statt.Die Tagung richtete sich an Planer, Anwender, Fachbetriebe, Sachverständige und Behörden. Es war das Ziel, über den derzeitigen Stand in der Befestigungstechnik zu informieren und die richtige, zulassungskonforme Planung und Anwendung von Dübeln und Verankerungen vorzustellen. Über 110 Teilnehmer folgten der Einladung nach Berlin, um sich intensiv über die Entwicklung der Zulassungen in der Befestigungstechnik zu informieren.Unter Moderation von Dr.‐Ing. habil. Klaus Block (TU Dortmund) und Dipl.‐Ing. Georg Feistel (DIBt) wurden in acht Vorträgen die aktuellsten Entwicklungen im Zulassungswesen der Befestigungstechnik aufgezeigt und dazu folgende Themen umfassend erläutert:– Bauaufsichtliche Grundlagen, Zulassungen in der Befestigungstechnik im Wandel Europas– Zulassungen für Verbundanker – eine Übersicht über Produkte und Bemessung– Vorgehängte hinterlüftete Fassaden – Befestigungen und Verankerungen– Kunststoffdübel – neue Zulassungsgeneration– Bemessen von Dübeln unter zyklischen Einwirkungen– Vermeidbare Qualitätseinbuße – Dübel auf dem Weg vom Hersteller zur Baustelle– Neues Bemessungskonzept für Ankerschienen – von abZ zur ETA– Einbetonierte Verankerungen
Countertransference reactions to adolescents with eating disorders: Relationships to clinician and patient factors
Authors: Satir Dana A. , Thompson‐Brenner Heather , Boisseau Christina L. , Crisafulli Michele A. 
Published: February 2009

AbstractObjective:Clinical report suggests that therapists have strong and sometimes difficult‐to‐manage reactions to patients with eating disorders (EDs); however, systematic research is largely absent. The purpose of this study was to explore the emotional responses, or countertransference (CT) reactions, clinicians experience when working with patients with EDs, and to identify clinician, patient, and therapy variables associated with these responses.Method:One hundred twenty clinicians reported on multiple variables related to an adolescent female patient they were treating for an ED.Results:Six patterns of reactions were identified: angry/frustrated, warm/competent, aggressive/sexual, failing/incompetent, bored/angry at parents and overinvested/worried feelings. The factors showed meaningful relationships across clinician demographics, patient characteristics, and treatment techniques.Discussion:Overall, clinician's reactions were most frequently associated with the clinician's gender, patient's level of functioning and improvement during treatment, and patient personality style. These issues have important implications for treatment, training and supervision. © 2009 by Wiley Periodicals, Inc. Int J Eat Disord 2009
Treating childhood obesity: Family background variables and the child's success in a weight‐control intervention
Authors: Pott Wilfried , Albayrak Özgür , Hebebrand Johannes , Pauli‐Pott Ursula 
Published: February 2009

AbstractObjectiveTo analyze whether caregiver and family characteristics predict success in a family‐based lifestyle intervention program for children and adolescents.MethodParticipants were 111 overweight and obese children (7–15 years) who attended a family‐based weight‐reduction program. Body mass index (BMI) and BMI standard deviation scores (BMI‐SDS) of index child, and BMI of family members, family adversity characteristics, depression, and attachment attitudes of the primary caregiver were assessed.ResultsRisk of nonresponse (≤5% reduction of BMI‐SDS or dropout) was elevated in older children, cases with obese sibling(s), maternal depression, and avoidant attachment attitude. In a logistic regression analysis, maternal depression, attachment attitude, and age of index child explained common variance whereas the presence of obese siblings explained unique variance in nonresponding.DiscussionTo meet the specific needs of all participating families and to prevent the discouraging experience of failure in weight‐control interventions, our data suggest that special support should be provided to adolescents with obese siblings, and cases of maternal depression, and avoidant attachment attitude. © 2009 by Wiley Periodicals, Inc. Int J Eat Disord 2009
DMBT1 functions as pattern‐recognition molecule for poly‐sulfated and poly‐phosphorylated ligands
Authors: End Caroline , Bikker Floris , Renner Marcus , Bergmann Gaby , Lyer Stefan , Blaich Stephanie , Hudler Melanie , Helmke Burkhard , Gassler Nikolaus , Autschbach Frank , Ligtenberg Antoon J. M , Benner Axel , Holmskov Uffe , Schirmacher Peter , Nieuw Amerongen Arie V. , Rosenstiel Philip , Sina Christian , Franke Andre , Hafner Mathias , Kioschis Petra , Schreiber Stefan , Poustka Annemarie , Mollenhauer Jan 
Published: February 2009

AbstractDeleted in malignant brain tumors 1 (DMBT1) is a secreted glycoprotein displaying a broad bacterial‐binding spectrum. Recent functional and genetic studies linked DMBT1 to the suppression of LPS‐induced TLR4‐mediated NF‐κB activation and to the pathogenesis of Crohn's disease. Here, we aimed at unraveling the molecular basis of its function in mucosal protection and of its broad pathogen‐binding specificity. We report that DMBT1 directly interacts with dextran sulfate sodium (DSS) and carrageenan, a structurally similar sulfated polysaccharide, which is used as a texturizer and thickener in human dietary products. However, binding of DMBT1 does not reduce the cytotoxic effects of these agents to intestinal epithelial cells in vitro. DSS and carrageenan compete for DMBT1‐mediated bacterial aggregation via interaction with its bacterial‐recognition motif. Competition and ELISA studies identify poly‐sulfated and poly‐phosphorylated structures as ligands for this recognition motif, such as heparansulfate, LPS, and lipoteichoic acid. Dose–response studies in Dmbt1−/− and Dmbt1+/+ mice utilizing the DSS‐induced colitis model demonstrate a differential response only to low but not to high DSS doses. We propose that DMBT1 functions as pattern‐recognition molecule for poly‐sulfated and poly‐phosphorylated ligands providing a molecular basis for its broad bacterial‐binding specificity and its inhibitory effects on LPS‐induced TLR4‐mediated NF‐κB activation.
Thermodynamic Properties of Nd1+xBa2–x­Cu3Oy Compounds and Their Application for Optimizing the Synthesis of Superconducting Materials
Authors: Zelenina Ludmila N. , Chusova Tamara P. , Matskevich Nata I. , Naumov Victor N. , Stenin Yuri G. , Krabbes Gernot 
Published: February 2009

AbstractThe standard enthalpies of formation from oxides of Nd1+xBa2–xCu3Oy, where x = 0–0.8 and y = 6.65–7.24, were determined by using dissolution enthalpies of these compounds measured by solution calorimetry. The temperature dependence of the heat capacity of NdBa2Cu3O6.87 wasmeasured over the range 5–320 K by adiabatic calorimetry. The absolute entropy, S°(T), the enthalpy of heating, H°(T K) – H°(0 K), and the reduced Gibbs energy, Φ°(T) = S°(T) – [H°(T) – H°(0)]/T, were calculated by using the temperature dependence of Cp in the interval 0–320 K. The heat capacity and absolute entropy of NdBa2Cu3O6.87 over the temperature range 320–1380 K were estimated by methods of quantum chemistry with use of the temperature dependence of Cp at low temperatures. A thermodynamic simulation was performed on the basis of the obtained data, and the conditions for synthesis of the NdBa2Cu3O6.9 phase were determined.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Novel Lactone Chamigrene‐Derived Metabolites from Laurencia majuscula
Authors: Díaz‐Marrero Ana R. , Brito Inmaculada , de la Rosa José M. , D'Croz Luis , Fabelo Oscar , Ruiz‐Pérez Catalina , Darias José , Cueto Mercedes 
Published: February 2009

AbstractGomerolactones A–D along with the known majusculone, obtusol, and elatol were isolated from Laurencia majuscula, and their structures were determined spectroscopically. With the aid of Pirkle's reagent at low temperature, NMR spectroscopy was used to determine the absolute configuration at the ring closure carbon atom of a α‐alkylidene‐γ‐lactone and an α‐alkylidene‐δ‐lactone unit embedded in the chamigrene network of compounds 1 and 2, respectively. The absolute stereochemistry of compounds 3 and 4 was determined by X‐ray analysis. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)