bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blended intensive programme’s implementation in dental education: post-pandemic evolution of learning
Authors: Duś-Ilnicka Irena , Paradowska-Stolarz Anna , Mazur Marta , Radwan-Oczko Małgorzata , Perra Andrea , Paula Vanessa Salete de , Ward Laura Sterian , Valente Nicola Alberto , Firkova Elena , Karteva Teodora , Jorda Lucia Miralles , Sousa Gomes Pedro de , Dominiak Marzena 
Published: March 2024

AbstractBlended Intensive Programmes (BIP’s) represent a valuable tool for gathering knowledge and summarising the latest trends in medicine and dentistry. Blended education has been found, even before the COVID-19 pandemic, to increase the level of education and stimulate effective learning for postgraduate healthcare professionals. Interprofessional education is critical for preparing students to enter the health workforce, where teamwork and collaboration are important competencies. This article outlines the key points of the Blended Intensive Programme’s implementation in dental education organised by Wroclaw Medical University in Poland. BIP involved professors from 12 universities or research institutions from Europe and South America and 28 participants from 8 countries. The course was taught remotely and in person. In addition, it included a visit to the university and practical classes with artificial simulation and practice in dentistry. A structured questionnaire enabled measuring the evaluation of students’ perception of the COVID-19 education before and after the pandemic. The European Region Action Scheme for the Mobility of University Students (ERASMUS) was fundamental to carrying out the BIP with the participation of several countries, allowing the exchange of knowledge, assessing the impact of the pandemic on dental universities, and strengthening international collaborations and the future project of research, education and clinical assistance. We conclude that hybrid teaching programmes broaden the learning spectrum in dental studies by allowing transnational and interdisciplinary approaches that make students aware of the importance of their work within the framework of the general health approach, as this differs from country to country.
Novel inhalation therapy in pulmonary fibrosis: principles, applications and prospects
Authors: Zheng Meiling , Zhu Wei , Gao Fei , Zhuo Yu , Zheng Mo , Wu Guanghao , Feng Cuiling 
Published: March 2024

AbstractPulmonary fibrosis (PF) threatens millions of people worldwide with its irreversible progression. Although the underlying pathogenesis of PF is not fully understood, there is evidence to suggest that the disease can be blocked at various stages. Inhalation therapy has been applied for lung diseases such as asthma and chronic obstructive pulmonary disease, and its application for treating PF is currently under consideration. New techniques in inhalation therapy, such as the application of microparticles and nanoparticles, traditional Chinese medicine monomers, gene therapy, inhibitors, or agonists of signaling pathways, extracellular vesicle interventions, and other specific drugs, are effective in treating PF. However, the safety and effectiveness of these therapeutic techniques are influenced by the properties of inhaled particles, biological and pathological barriers, and the type of inhalation device used. This review provides a comprehensive overview of the pharmacological, pharmaceutical, technical, preclinical, and clinical experimental aspects of novel inhalation therapy for treating PF and focus on therapeutic methods that significantly improve existing technologies or expand the range of drugs that can be administered via inhalation. Although inhalation therapy for PF has some limitations, the advantages are significant, and further research and innovation about new inhalation techniques and drugs are encouraged.
Graphical Abstract
Revolutionizing lymph node metastasis imaging: the role of drug delivery systems and future perspectives
Authors: Cai Ze-Min , Li Zi-Zhan , Zhong Nian-Nian , Cao Lei-Ming , Xiao Yao , Li Jia-Qi , Huo Fang-Yi , Liu Bing , Xu Chun , Zhao Yi , Rao Lang , Bu Lin-Lin 
Published: March 2024

AbstractThe deployment of imaging examinations has evolved into a robust approach for the diagnosis of lymph node metastasis (LNM). The advancement of technology, coupled with the introduction of innovative imaging drugs, has led to the incorporation of an increasingly diverse array of imaging techniques into clinical practice. Nonetheless, conventional methods of administering imaging agents persist in presenting certain drawbacks and side effects. The employment of controlled drug delivery systems (DDSs) as a conduit for transporting imaging agents offers a promising solution to ameliorate these limitations intrinsic to metastatic lymph node (LN) imaging, thereby augmenting diagnostic precision. Within the scope of this review, we elucidate the historical context of LN imaging and encapsulate the frequently employed DDSs in conjunction with a variety of imaging techniques, specifically for metastatic LN imaging. Moreover, we engage in a discourse on the conceptualization and practical application of fusing diagnosis and treatment by employing DDSs. Finally, we venture into prospective applications of DDSs in the realm of LNM imaging and share our perspective on the potential trajectory of DDS development.
Graphical Abstract
Quality of life-related and non-quality of life-related issues in ICU survivors and non-ICU-treated controls: a multi-group exploratory factor analysis
Authors: Malmgren Johan , Lundin Stefan , Waldenström Ann-Charlotte , Rylander Christian , Johannesson Elias 
Published: March 2024

Abstract
Background
Quality of life (QoL) is a key outcome measure in healthcare. However, the heterogeneity in its definitions presents challenges in the objective evaluation of improvement. Universal questionnaires, tailored for a broad demographic group, inadequately represent the unique experiences of intensive care unit (ICU) survivors, including a lack of ability to discriminate issues related to QoL from issues that do not.

Methods
Using a 218-item, 13-domain provisional questionnaire, we assessed 395 adult ICU survivors, with a minimum 72-h stay at one of three Swedish university hospital ICUs, at 6 months to three years post-discharge. Their responses were compared to those of 195 controls, matched for age and sex and randomly recruited from the Swedish Population Registry. By multi-group exploratory factor analysis, we compared dimensionality in QoL perceptions between the two groups, emphasising patterns of correlation to 13 domain-specific QoL questions. Model fit was assessed using information criteria. Internal consistency reliability for each scale was determined using McDonald’s omega or Cronbach’s alpha. All analyses were conducted using Mplus, applying full information maximum likelihood to handle missing data.

Results
All domains except Cognition had a subset of questions correlating to the domain-specific QoL question in at least the ICU survivor group. The similarity between the two groups varied, with Physical health, Sexual health and Gastrointestinal (GI) functions mainly correlating the same issues to QoL in the two groups. In contrast, Fatigue, Pain, Mental health, activities of daily living, Sleep, Sensory functions and Work life showed considerable differences. In all, about one-fourth of the issues correlated to QoL in the ICU survivor group and about one-tenth of the issues in the control group.

Conclusions
We found most issues experienced by ICU survivors to be unrelated to quality of life. Our findings indicate that the consequences of post-ICU issues may play a more significant role in affecting QoL than the issues themselves; issues restricting and affecting social life and work life were more related to QoL in ICU survivors than in non-ICU-treated controls. Caution is advised before associating all post-ICU problems with an effect on quality of life.
Trial registration: ClinicalTrials.gov Ref# NCT02767180; Registered 28 April 2016.
Factors associated with overall survival in breast cancer patients with leptomeningeal disease (LMD): a single institutional retrospective review
Authors: Wallace Gerald , Kundalia Ronak , Vallebuona Ethan , Cao Biwei , Kim Youngchul , Forsyth Peter , Soyano Aixa , Smalley Inna , Pina Yolanda 
Published: March 2024

Abstract
Background
Breast cancer-related leptomeningeal disease (BC-LMD) is a dire diagnosis for 5–8% of patients with breast cancer (BC). We conducted a retrospective review of BC-LMD patients diagnosed at Moffitt Cancer Center from 2011 to 2020, to determine the changing incidence of BC-LMD, factors which are associated with the progression of BC CNS metastasis to BC-LMD, and factors which are associated with OS for patients with BC-LMD.

Methods
Patients with BC and brain/spinal metastatic disease were identified. For those who eventually developed BC-LMD, we used Kaplan–Meier survival curve, log-rank test, univariable, and multivariate Cox proportional hazards regression model to identify factors affecting time from CNS metastasis to BC-LMD and OS.

Results
128 cases of BC-LMD were identified. The proportion of BC-LMD to total BC patients was higher between 2016 and 2020 when compared to 2011–2015. Patients with HR+ or HER2 + BC experienced longer times between CNS metastasis and LMD than patients with triple-negative breast cancer (TNBC). Systemic therapy and whole-brain radiation therapy (WBRT) was associated with prolonged progression to LMD in all patients. Hormone therapy in patients with HR + BC were associated with a delayed BC-CNS metastasis to LMD progression. Lapatinib treatment was associated with a delayed progression to LMD in patients with HER2 + BC. Patients with TNBC-LMD had shorter OS compared to those with HR + and HER2 + BC-LMD. Systemic therapy, intrathecal (IT) therapy, and WBRT was associated with prolonged survival for all patients. Lapatinib and trastuzumab therapy was associated with improved OS in patients with HER2 + BC-LMD.

Conclusions
Increasing rates of BC-LMD provide treatment challenges and opportunities for clinical trials. Prospective trials testing lapatinib and/or similar tyrosine kinase inhibitors, IT therapies, and combination treatments are urgently needed.
Exploring the dynamic interplay between exosomes and the immune tumor microenvironment: implications for breast cancer progression and therapeutic strategies
Authors: Safaei Sahar , Fadaee Manouchehr , Farzam Omid Rahbar , Yari Amirhossein , Poursaei Elham , Aslan Cynthia , Samemaleki Sahar , Shanehbandi Dariush , Baradaran Behzad , Kazemi Tohid 
Published: March 2024

AbstractBreast cancer continues to pose a substantial worldwide health concern, demanding a thorough comprehension of the complex interaction between cancerous cells and the immune system. Recent studies have shown the significant function of exosomes in facilitating intercellular communication and their participation in the advancement of cancer. Tumor-derived exosomes have been identified as significant regulators in the context of breast cancer, playing a crucial role in modulating immune cell activity and contributing to the advancement of the illness. This study aims to investigate the many effects of tumor-derived exosomes on immune cells in the setting of breast cancer. Specifically, we will examine their role in influencing immune cell polarization, facilitating immunological evasion, and modifying the tumor microenvironment. Furthermore, we explore the nascent domain of exosomes produced from immune cells and their prospective involvement in the prevention of breast cancer. This paper focuses on new research that emphasizes the immunomodulatory characteristics of exosomes produced from immune cells. It also explores the possibility of these exosomes as therapeutic agents or biomarkers for the early identification and prevention of breast cancer. The exploration of the reciprocal connections between exosomes formed from tumors and immune cells, together with the rising significance of exosomes derived from immune cells, presents a potential avenue for the advancement of novel approaches in the field of breast cancer therapy and prevention.
FixNCut: single-cell genomics through reversible tissue fixation and dissociation
Authors: Jiménez-Gracia Laura , Marchese Domenica , Nieto Juan C. , Caratù Ginevra , Melón-Ardanaz Elisa , Gudiño Victoria , Roth Sara , Wise Kellie , Ryan Natalie K. , Jensen Kirk B. , Hernando-Momblona Xavier , Bernardes Joana P. , Tran Florian , Sievers Laura Katharina , Schreiber Stefan , van den Berge Maarten , Kole Tessa , van der Velde Petra L. , Nawijn Martijn C. , Rosenstiel Philip , Batlle Eduard , Butler Lisa M. , Parish Ian A. , Plummer Jasmine , Gut Ivo , Salas Azucena , Heyn Holger , Martelotto Luciano G. 
Published: March 2024

AbstractThe use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.
Hyperspectral estimation method for deterioration of rock carvings in the humid regions of southern China
Authors: Chen Chiwei , Yang Haiqing , Li Xingyue , Zhao Gang , Ni Jianghua 
Published: March 2024

Abstract
Deterioration development is a recognized worldwide threat to rock carvings, especially in humid regions of southern China. Cultural heritage managers thus require precise identification of different deterioration patterns and conduct comprehensive assessments. However, the quantitative analysis of deterioration patterns is limited due to the severe impact of temperature and humidity on rock carvings. Additionally, the current research on the different deterioration patterns is independent, and the corresponding systematic framework is vague. Based on this, the hyperspectral response is constructed to evaluate the various deterioration patterns using spectral index and intelligent model. Firstly, the remarkable correlation between the feldspar content and the deterioration patterns of rock carvings with the influence of environmental factors is investigated by mineralogical analysis. Secondly, combined with microscopic and mineralogical characteristics, the extracted deterioration characteristics are qualitatively screened. Then, a novel spectral index characterizing the correlation between image grayscale and spectral reflectance is proposed by introducing dynamic correction, and the optimal wavelength combination is applied to identify the distribution of deterioration patterns. Consequently, the quantitative screening of deterioration patterns can be realized. After that, the WOA-XGBoost model exhibits better performance in the classification of deterioration patterns. Finally, the influence of different deterioration patterns on rock carvings is quantified by integrating the deterioration index reflected by chemical composition and the proportion of deterioration pattern distribution identified by the spectral response. In the regional deterioration assessment of Dazu Rock Carvings, biological colonization and surface morphological changes have the highest proportion and degree of deterioration, which is worthy of attention in the protection of rock carvings in this region.
Combined metagenomics and metabolomic analysis of microbial community structure and metabolic function in continuous soybean cropping soils of Songnen Plain, China
Authors: Xu Letian , Jin Shun , Su Yue , Lyu Xiaochen , Yan Shuangshuang , Wang Chang , Cao Liang , Yan Chao , Ma Chunmei 
Published: March 2024

AbstractContinuous cropping has a negative effect on soybean yield. In this study, a positioning experiment was conducted starting in 2015, with three treatments: maize–soybean rotation (SMR), 2-year maize, 2-year soybean rotation cropping (SC2), and 8-year soybean continuous cropping (SC8). We determined soybean yields (2015–2022) and analyzed soil microbial communities, functions, and metabolites composition in the 0–20 cm tillage layer using metagenomics technology and GC–MS technology during soybean flowering in 2022. Results indicated that continuous cropping (SC8) significantly reduced soybean yield compared to crop rotation (SMR) during the experimental period, while SC8 showed higher yield than SC2 in 2022. Compared to SMR, SC8 significantly increased soil N content and significantly decreased pH and TP, AP, and AK content. However, the pH and AK contents of SC8 were significantly higher than those of SC2. LeFSe analysis showed that Friedmanniella, Microlunatus, Nitrososphaera, Rubrobacter, Geodermatophilus, Nitriliruptor were enriched in SC8. Gaiella, Sphaerobacter, Methyloceanibacter were enriched in SC2. Sphingomonas, Cryobacterium, Marmoricola, Haliangium, Arthrobacter, Ramlibacter, Rhizobacter, Pseudolabrys, Methylibium, Variovorax were enriched in SMR. And the relative abundance of Cryobacterium, Marmoricola, Haliangium, Arthrobacter, Ramlibacter, Rhizobacter, Methylibium, Variovorax was significantly positively correlated with yield, while the relative abundance of Gaiella and Sphaerobacter was significantly negatively correlated with yield. SC8 significantly increased the abundance of genes in nitrogen metabolism and significantly decreased the abundance of genes related to phosphorus and potassium metabolism compared with SMR. However, the abundance of genes in potassium metabolism was significantly higher in SC8 than in SC2. Metabolomic analysis showed that compared to SMR, SC8 decreased the abundance of carbohydrates, ketones, and lipid. However, the abundance of carbohydrates, ketones, and lipid was significantly higher in SC8 than in SC2. Mantel test showed that soil pH and AK significantly affected soil microbial community, function, and metabolite composition. Correlation analysis showed significant correlation between soil metabolites and microorganisms, metabolic functions.
Graphical Abstract
Electron microprobe dating of lunar zirconolite
Authors: Park Changkun , Kim Hwayoung 
Published: March 2024

AbstractZirconolite is an accessary mineral occurred in the lunar basaltic and granitic rocks and contains relatively high contents of U, Th, and Pb, which is attractive for age dating. However, very few studies have reported the crystallization ages of lunar zirconolites because of the challenge of dating lunar zirconolites due to their fine-grained size and irregular shape. In this study, we analyzed zirconolites in a granitic clast of the lunar meteorite DEW 12007 using an electron microprobe. MAN (mean atomic number) background, peak interference, and blank corrections were applied to 31 elements including U, Th, and Pb, and REEs, to obtain high-precision and high-accuracy chemical data of the zirconolites. The electron microprobe age of the zirconolites is determined to be 4332 ± 14 Ma (2σ, n = 20), which is consistent with the U–Pb age (4340.9 ± 7.5 Ma; 2σ) of zircon grains from the same clast measured by an ion microprobe. The precision and accuracy achieved in this study represents a notable advance compared to previously reported electron microprobe ages of lunar zirconolites. This suggests that electron microprobe dating may be applicable to extraterrestrial materials, especially for microscopic U-Th-Pb-containing minerals in the samples returned from the Moon and Mars.
Highly sensitive plasmonic paper substrate fabricated via amphiphilic polymer self-assembly in microdroplet for detection of emerging pharmaceutical pollutants
Authors: Sharipov Mirkomil , Kakhkhorov Sarvar A. , Tawfik Salah M. , Azizov Shavkatjon , Liu Hong-Guo , Shin Joong Ho , Lee Yong-Ill 
Published: March 2024

AbstractWe report an innovative and facile approach to fabricating an ultrasensitive plasmonic paper substrate for surface-enhanced Raman spectroscopy (SERS). The approach exploits the self-assembling capability of poly(styrene-b-2-vinyl pyridine) block copolymers to form a thin film at the air-liquid interface within the single microdroplet scale for the first time and the subsequent in situ growth of silver nanoparticles (AgNPs). The concentration of the block copolymer was found to play an essential role in stabilizing the droplets during the mass transfer phase and formation of silver nanoparticles, thus influencing the SERS signals. SEM analysis of the morphology of the plasmonic paper substrates revealed the formation of spherical AgNPs evenly distributed across the surface of the formed copolymer film with a size distribution of 47.5 nm. The resultant enhancement factor was calculated to be 1.2 × 107, and the detection limit of rhodamine 6G was as low as 48.9 pM. The nanohybridized plasmonic paper was successfully applied to detect two emerging pollutants—sildenafil and flibanserin—with LODs as low as 1.48 nM and 3.45 nM, respectively. Thus, this study offers new prospects for designing an affordable and readily available, yet highly sensitive, paper-based SERS substrate with the potential for development as a lab-on-a-chip device.
Semi-customized three-dimensional ultra-fine titanium meshes in guided bone regeneration for implant therapy in severe alveolar bone defect: a case report
Authors: Park Dae-Ho , Jun Jong-Hun , Yun Seo-Hyoung , Choi Baek-Sun , Fiorellini Joseph P. , Tallarico Marco , Hwang Kyung-Gyun , Park Chang-Joo 
Published: March 2024

AbstractThis case report provides a detailed description of a simple and fast bone regeneration procedure using a semi-customized three-dimensional ultra-fine titanium mesh. A 50-year-old male with a severe vertical and horizontal bone defect in the anterior mandible underwent implant treatment in a staged approach. The autologous bone was combined with a xenograft, and the mixture was grafted to augment the bone defect and covered with semi-customized ultra-fine titanium meshes, which were selected among its various types according to size and configuration of the bone defect, directly connected and immobilized on the tenting screws with minimal shaping. In a postoperative 6 months re-entry surgery, the performed titanium meshes were removed, implants were placed, and a bone core biopsy was obtained that demonstrated satisfactory new bone formation. Finally, two months later, the definitive prosthesis was installed. This semi-customized ultra-fine titanium mesh could help an implant clinician obtain more predictable results in the guided bone regeneration (GBR).