bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Highly Practical Amino Acid Synthesis with Designer Chiral Phase Transfer Catalysts
Authors: Maruoka Keiji 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: General Strategies for the Synthesis of Iminosugars and New Approaches Towards Iminosugar Libraries
Authors: La Ferla Barbara , Cipolla Laura , Nicotra Francesco 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Properties and Degradation of Selected Polysaccharides: Hyaluronan and Chitosan
Authors: Rinaudo M. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Advances in Development of Methods for the Synthesis of Dual Selective Serotonin and Norepinephrine Reuptake Inhibitors (SSNRIs) [Venlafaxine Hydrochloride (Effexor), Milnacipran Hydrochloride (Ixel, Dalcipran) and Duloxetine Hydrochloride (Cymbalta)]
Authors: Pineiro‐Nunez Marta 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hydrogen‐Rich Boron‐Containing Materials for Hydrogen Storage
Authors: Wang Ping , Kang Xiang‐dong 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Towards Magnetic Molecule and Reagent Separation in Organic Synthesis: Development and Use of Covalently Functionalized Nanomagnets
Authors: Halim Samuel C. , Stark Wendelin J. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Fibroblast elongation and dendritic extensions in constrained versus unconstrained microtissues
Authors: Dean Dylan M. , Rago Adam P. , Morgan Jeffrey R. 
Published: January 2009

AbstractCytoskeletal tension is fundamental to many biological processes, including germ layer sorting during embryogenesis [Krieg et al., 2008]. In vitro, such tension influences cell sorting in self‐assembled, 3D microtissues and can be of sufficient magnitude to cause complex‐shaped microtissue failure [Dean et al., 2007]. To examine the process of failure under cell‐derived tension, we subjected normal human fibroblasts (NHFs) to directed self‐assembly [Dean et al., 2007] in micro‐molds designed to yield self‐constraining microtissues. As cells contracted in this assay, the constrained microtissues narrowed, thinned and ultimately failed at their midpoints. By adding small numbers of GFP+ cells, changes in cell movement and morphology were assessed and compared to those of unconstrained microtissues. We found that cells formed numerous dendritic extensions within an hour of self‐assembly and retracted these extensions as they elongated up to 30 times their initial diameter (∼600 μm) just prior to failure. Surprisingly, significant coordination in cell motility was observed over large distances within microtissues. Pharmacologic interventions showed that failure was myosin II and Rho kinase dependent and inhibition of failure resulted in shorter cells with greater numbers of extensions. These findings further our understanding of cellular self‐assembly and introduce the use of GFP+ cells with directed self‐assembly as a scaffold‐free analogue to fibroblast‐populated collagen gels (FPCGs). Cell Motil. Cytoskeleton 2009. © 2009 Wiley‐Liss, Inc.
Centromere protein H is a novel prognostic marker for human nonsmall cell lung cancer progression and overall patient survival
Authors: Liao Wen‐Ting , Wang Xi , Xu Li‐Hua , Kong Qing‐Li , Yu Chun‐Ping , Li Man‐Zhi , Shi Ling , Zeng Mu‐Sheng , Song Li‐Bing 
Published: January 2009

AbstractBACKGROUND:Lung cancer is 1 of the leading causes of cancer death worldwide, and the high mortality from this disease is caused mainly by the lack of efficient diagnostic strategies for early‐stage lung cancer. The objective of the current study was to investigate the expression pattern and clinicopathologic significance of centromere protein H (CENP‐H) in patients with nonsmall cell lung cancer (NSCLC).METHODS:The expression profile of CENP‐H in normal lung epithelial cells, NSCLC cell lines, NSCLC tissues, and adjacent noncancerous lung tissues were detected by reverse transcription‐polymerase chain reaction (RT‐PCR), real‐time RT‐PCR, and Western blot analysis. The expression level of CENP‐H in 223 NSCLC tissues was measured by immunohistochemistry staining. Statistical analysis was performed to evaluate the clinicopathologic significance of CENP‐H.RESULTS:The expression level of CENP‐H was much higher in cancer cell lines and lung cancer tissues than that in normal cells and adjacent noncancerous lung tissues, respectively. Immunohistochemical analysis revealed positive CENP‐H expression in 118 of 223 NSCLC tissues (52.9%). Statistical analysis revealed that CENP‐H expression was correlated strongly with clinical stage (P=.018), tumor classification (P=.03), and Ki‐67 expression (P < .001). Patients with lower CENP‐H expression had better overall survival than patients with higher CENP‐H expression. Further analysis suggested that CENP‐H could predict prognosis only in patients with early‐stage disease. Multivariate analysis suggested that CENP‐H expression was an independent prognostic marker for survival in patients with NSCLC.CONCLUSIONS:The current results demonstrated that high CENP‐H protein expression was related to poor outcome in patients with NSCLC. CENP‐H may be used as a prognostic biomarker for patients lung patients, especially those with early‐stage NSCLC. Cancer 2009. © 2009 American Cancer Society.
Concurrent lobular neoplasia increases the risk of ipsilateral breast cancer recurrence in patients with ductal carcinoma in situ treated with breast‐conserving therapy
Authors: Rudloff Udo , Brogi Edi , Brockway Julia P. , Goldberg Jessica I. , Cranor Milicent , Wynveen Christine A. , Nehhozina Tatjana , Reiner Anne S. , Patil Sujata , Van Zee Kimberly J. 
Published: January 2009

AbstractBACKGROUND:Multiple clinicopathologic factors have been analyzed for their association with an increased risk of ipsilateral breast tumor recurrence (IBTR) after women receive breast‐conserving treatment (BCT) for ductal carcinoma in situ (DCIS). The reported incidence of proliferative lesions, such as atypical ductal hyperplasia (ADH), columnar cell changes (CCC), and lobular neoplasia associated with breast cancer, has been as high as 23%; however, the relevance of these lesions on the natural history of DCIS and the risk of IBTR remains unknown.METHODS:Two hundred ninety‐four patients with DCIS who received BCT between 1991 and 1995 were identified from the authors' institutional database. Slides were reviewed by a dedicated breast pathologist with particular attention to the presence of lobular neoplasia, ADH, and CCC. The actuarial 5‐, 10‐, and 15‐year IBTR rates were calculated using the Kaplan‐Meier method and were compared using the log‐rank test.RESULTS:Concurrent lobular neoplasia was present in 41 of 294 patients (14%), ADH was present in 37 of 294 patients (13%), and CCC was present in 71 of 294 patients (24%). The median follow‐up was 11 years. IBTR occurred in 40 of 227 patients without lobular neoplasia (18%) versus 15 of 41 patients with lobular neoplasia (37%; P=.005; hazard ratio [HR], 2.49). The 5‐, 10‐, and 15‐year cumulative incidence rates of IBTR were twice as high in women who had DCIS and lobular neoplasia compared with women who had DCIS alone (P=.002). Concomitant ADH (HR, 1.53) and CCC (HR, 1.24) were not associated significantly with IBTR (P=.20 and P=.44, respectively).CONCLUSIONS:Concurrent lobular neoplasia is associated with a significantly higher risk of IBTR in women with DCIS who received BCT. Women with coexisting DCIS and lobular neoplasia who receive BCT should consider using additional risk‐reducing strategies. Cancer 2009. © 2009 American Cancer Society.
Phase 1 study of oxaliplatin and irinotecan in pediatric patients with refractory solid tumors
Authors: McGregor Lisa M. , Spunt Sheri L. , Furman Wayne L. , Stewart Clinton F. , Schaiquevich Paula , Krailo Mark D. , Speights RoseAnne , Ivy Percy , Adamson Peter C. , Blaney Susan M. 
Published: January 2009

AbstractBACKGROUND:For this report, the authors estimated the maximum tolerated dose (MTD) and investigated the toxicities of oxaliplatin combined with irinotecan in children with refractory solid tumors.METHODS:Oxaliplatin was administered on Days 1 and 8 in combination with irinotecan on Days 1 through 5 and Days 8 through 12 of a 21‐day cycle. An oral cephalosporin was administered daily to ameliorate irinotecan‐associated diarrhea. Pharmacokinetic studies of oxaliplatin and uridine diphosphate glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1) genotyping were performed.RESULTS:Thirteen patients were enrolled. Dose‐limiting diarrhea (n = 3), serum lipase elevation (n = 3), serum amylase elevation (n = 2), colitis, abdominal pain, and headache (n = 1 each) occurred at the first dose level (oxaliplatin at a dose of 60 mg/m2; irinotecan at a dose of 20 mg/m2). Only 1 of 7 patients who received reduced doses of both agents (40 mg/m2/dose oxaliplatin; 15 mg/m2/dose irinotecan) experienced a dose‐limiting toxicity (DLT): diarrhea. When the oxaliplatin dose was re‐escalated (60 mg/m2) with irinotecan at a dose of 15 mg/m2, 2 of 3 patients had a DLT (1 episode of diarrhea, 1 episode of hypokalemia). Myelosuppression was minimal. One patient had a complete response, and another patient had stable disease for 6 cycles of therapy. The median oxaliplatin area under the concentration versus time curve (AUC0→∞) was 5.9 μg · hour/mL (range, 1.8‐7.6 μg · hour/mL). The frequency of the 6/6, 6/7, and 7/7 UGT1A1 promoter genotypes was 5 of 10, 4 of 10, and 1 of 10, respectively.CONCLUSIONS:The oxaliplatin MTD was 40 mg/m2 per dose on Days 1 and 8 in combination with irinotecan 15 mg/m2 per dose on Days 1‐5 and Days 8‐12. There was some evidence of antitumor activity; however, severe toxicity, both expected (diarrhea) and unexpected (elevation in pancreatic enzymes), was observed. Cancer 2009. © 2009 American Cancer Society.
Formation of Cross‐Linked Chloroperoxidase Aggregates in the Pores of Mesocellular Foams: Characterization by SANS and Catalytic Properties
Authors: Jung Dirk , Paradiso Michelangelo , Wallacher Dirk , Brandt Astrid , Hartmann Martin 
Published: January 2009

AbstractNo escape: The formation of cross‐linked chloroperoxidase aggregates (CPO‐CLEAs) in the pores of mesocellular foam materials results in active biocatalysts that are more resistant to leaching than the conventional catalyst prepared by physisorption of chloroperoxidase. Small‐angle neutron scattering (SANS) experiments clearly confirm that the CPO‐CLEAs are located in the pores of the mesocellular foams.magnified image
Cytological features of signet‐ring cell carcinoma of the lung: Comparison with the goblet‐cell‐type adenocarcinoma of the lung
Authors: Tsuta Koji , Shibuki Yasuo , Maezawa Naoki , Tochigi Naobumi , Maeshima Akiko Miyagi , Sasajima Yuko , Asamura Hisao , Matsuno Yoshihiro 
Published: January 2009

AbstractSignet‐ring cell carcinoma (SRCC) and goblet‐cell‐type adenocarcinoma (GCA) are mucin‐producing lung adenocarcinomas. Primary SRCC shows an aggressive clinical course, whereas GCA shows infrequent distant metastasis, but more frequent intrapulmonary metastases resembling lobar pneumonia. To distinguish SRCC from GCA, this study investigated the respective cytological features of these lesions. We selected 10 cases each of SRCC and GCA from the archival imprint smears. We assessed them for the following 10 cytological features. Necrosis/debris was observed in 60% of the SRCC and 90% of the GCA. A mucinous background was observed in 10% of the SRCC and 90% of the GCA. Significant inflammation was observed in none of the SRCC and 80% of the GCA. Stromal cluster was observed in 30% of the SRCC and 70% of the GCA. Nuclear overlapping was observed in 50% of the SRCC and in all of the GCA. Single tumor cells were observed in 80% of the SRCC and 10% of the GCA. Honeycomb‐like cluster was observed in none of the SRCC and 80% of the GCA. Prominent nucleolus was observed in 50% of the SRCC and 40% of the GCA. Nuclear membrane irregularity was observed in 70% of SRCC and 60% of the GCA. Nuclear pleomorphism was observed in all of the SRCC and none of the GCA. The cytological features of SRCC were the presence of single tumor cells and nuclear pleomorphism, whereas that of GCA were the presence of abundant mucin and significant inflammation in the background, and a honeycomb‐like cluster. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.
Anatomy and development of the nervous system of Nematostella vectensis, an anthozoan cnidarian
Authors: Marlow Heather Q. , Srivastava Mansi , Matus David Q. , Rokhsar Daniel , Martindale Mark Q. 
Published: January 2009

AbstractNematostella vectensis, an anthozoan cnidarian, whose genome has been sequenced and is suitable for developmental and ecological studies, has a complex neural morphology that is modified during development from the larval to adult form. N. vectensis' nervous system is a diffuse nerve net with both ectodermal sensory and effector cells and endodermal multipolar ganglion cells. This nerve net consists of several distinct neural territories along the oral–aboral axis including the pharyngeal and oral nerve rings, and the larval apical tuft. These neuralized regions correspond to expression of conserved bilaterian neural developmental regulatory genes including homeodomain transcription factors and NCAMs. Early neurons and stem cell populations identified with NvMsi, NvELAV, and NvGCM, indicate that neural differentiation occurs throughout the animal and initiates prior to the conclusion of gastrulation. Neural specification in N. vectensis appears to occur through an independent mechanism from that in the classical cnidarian model Hydra. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009
13C/12C Ratios of endogenous urinary steroids investigated for doping control purposes
Authors: Piper Thomas , Flenker Ulrich , Mareck Ute , Schänzer Wilhelm 
Published: January 2009

AbstractIn order to detect the misuse of endogenous anabolic steroids such as testosterone by athletes a total of n = 1734 suspicious urine samples were investigated by gas chromatography/combustion/isotope ratio mass spectrometry throughout the years 2005, 2006 and 2007. The 13C/12C ratio of a target substance (androsterone, a testosterone metabolite) was compared to the 13C/12C ratio of an endogenous reference compound (11β‐hydroxyandrosterone).N = 1340 samples were investigated due to elevated testosterone/epitestosterone ratios, with n = 87 (6.5%) exceptional findings regarding their isotopic ratios. An additional n = 164 samples were investigated because of elevated dehydroepiandrosterone concentrations, with n = 2 (1.2%) exceptional findings. The remainder were subjected to isotope ratio analysis because of elevated androsterone levels or because this was requested by sports federations.Significant differences between female and male samples were found for the 13C/12C ratios of androsterone and 11β‐hydroxyandrosterone but not for samples taken in or out of competition.A further n = 645 samples originating from other World Anti‐Doping Agency accredited laboratories, mainly throughout Europe as well as South America, South Africa and Southeast Asia, were investigated. The 13C/12C ratios of the urinary steroids differ significantly for each geographical region, reflecting the dietary status of the individuals.The system stability over time has been tested by repeated injections of a standard solution and repeated processing of frozen stored blank urine. Despite a drift over time in absolute 13C/12C ratios, no significant change in the difference of 13C/12C (11β‐hydroxyandrosterone) minus 13C/12C (androsterone) could be observed. Copyright © 2009 John Wiley & Sons, Ltd. Copyright © 2009 John Wiley & Sons, Ltd.
Small interfering peptide (siP) for in vivo examination of the developing lung interactonome
Authors: Cohen J. Craig , Killeen Erin , Chander Avinash , Takemaru Ken‐Ichi , Larson Janet E. , Treharne Kate J. , Mehta Anil 
Published: January 2009

AbstractTo understand the role of reactive oxygen species in mechanosensory control of lung development a new approach to interfere with protein–protein interactions by means of a short interacting peptide was developed. This technology was used in the developing rodent lung to examine the role of NADPH oxidase (NOX), casein kinase 2 (CK2), and the cystic fibrosis transmembrane conductance regulator (CFTR) in stretch‐induced differentiation. Interactions between these molecules was targeted in an in utero system with recombinant adeno‐associated virus (rAAV) containing inserted DNA sequences that express a control peptide or small interfering peptides (siPs) specific for subunit interaction or phosphorylation predicted to be necessary for multimeric enzyme formation. In all cases only siPs with sequences necessary for a predicted normal function were found to interfere with assembly of the multimeric enzyme. A noninterfering control siP to nonessential regions or reporter genes alone had no effect. Physiologically, it was shown that siPs that interfered with the NOX‐CFTR‐CK2 complex that we call an “interactonome” affected markers of stretch‐induced lung organogenesis including Wnt/β‐catenin signaling. Developmental Dynamics 238:386–393, 2009. © 2009 Wiley‐Liss, Inc.
TheCaenorhabditis elegansheterochronic genelin‐14coordinates temporal progression and maturation in the egg‐laying system
Authors: Johnson Ryan W. , Liu Leah Y. , Hanna‐Rose Wendy , Chamberlin Helen M. 
Published: January 2009

AbstractHeterochronic genes function to ensure the timing of stage‐specific developmental events inC. elegans. Mutations in these genes cause certain developmental programs to be executed in a precocious or retarded manner. Canonical precocious (loss‐of‐function) and retarded (gain‐of‐function) mutations in thelin‐14gene lead to elimination or reiteration of larval stage‐specific cellular events. Here, we describe a hypomorphic, missense allele oflin‐14,sa485.lin‐14(sa485) hermaphrodites pass through normal larval stages, but exhibit asynchrony between vulval and gonadal maturation in the L4 larval stage. We show that a subtly precocious morphogenetic event in the vulva disrupts tissue synchrony and is followed by retarded vulval eversion. Additionally, uterine uv1 cell differentiation is retarded inlin‐14(sa485) animals that exhibit delayed vulval eversion. Together, these experiments outline a function for LIN‐14 in coordinating the temporal progression of development, which is separable from its role in regulating stage‐specific events duringC. eleganspostembryonic development. Developmental Dynamics 238:394–404, 2009. © 2009 Wiley‐Liss, Inc.
Fibroblast growth factor‐2 deficiency affects hippocampal spine morphology, but not hippocampal catecholaminergic or cholinergic innervation
Authors: Zechel Sabrina , Unsicker Klaus , von Bohlen und Halbach Oliver 
Published: January 2009

AbstractThe availability of fibroblast growth factor‐2 (FGF‐2) ‐deficient mice has permitted studying the role of endogenous FGF‐2. Several studies have reported that neocortical but not hippocampal neurons are lost in FGF‐2–deficient mice. Here, we show that neuronal densities within the basolateral amygdala are unaltered in FGF‐2−/− mice. Moreover, we provide evidence that FGF‐2 mutant mice display no obvious alterations in the catecholaminergic or cholinergic innervation of the hippocampus. With regard to the formation of dendritic spines, our studies reveal that endogenous FGF‐2 is not essential for hippocampal spinogenesis; however, FGF‐2 affects the length of individual spines. Such alterations in spine morphology may be related to disturbances in mental capacities or alterations in neuronal plasticity. Of interest, in this context, animal models of mental retardation develop no alterations in hippocampal spine densities, but display higher numbers of long dendritic spines. Thus, FGF‐2 may also affect learning and memory by altering spine morphology. Developmental Dynamics 238:343–350, 2009. © 2009 Wiley‐Liss, Inc.
Estimating the spatiotemporal pattern of volumetric growth rate from fate maps in chick limb development
Authors: Morishita Yoshihiro , Iwasa Yoh 
Published: January 2009

AbstractMorphogenesis is achieved through volumetric growth of tissue at a rate varying over space and time. The volumetric growth rate of each piece of tissue reflects the behaviors of constituent cells such as cell proliferation and death. Hence, clarifying its spatiotemporal pattern accurately is a key to bridge between cell behaviors and organ morphogenesis. We here propose a new method to estimate the spatiotemporal pattern of volumetric growth rate from fate map data with limited resolution on space and time by using a mathematical model. We apply the method to chick wing data along the proximodistal axis, and find that the volumetric growth pattern is biphasic: it is spatially uniform in earlier stages (until stage 23), but in later stages the volumetric growth occurs approximately 4.5 times as fast as in the distal region (within approximately 100 μm from the limb tip) than in the proximal region. Developmental Dynamics 238:415–422, 2009. © 2009 Wiley‐Liss, Inc.
C‐myc as a modulator of renal stem/progenitor cell population
Authors: Couillard Martin , Trudel Marie 
Published: January 2009

AbstractThe role of c‐myc has been well‐studied in gene regulation and oncogenesis but remains elusive in murine development from midgestation. We determined c‐myc function during kidney development, organogenesis, and homeostasis by conditional loss of c‐myc induced at two distinct phases of nephrogenesis, embryonic day (e) 11.5 and e17.5. Deletion of c‐myc in early metanephric mesenchyme (e11.5) led to renal hypoplasia from e15.5 to e17.5 that was sustained until adulthood (range, 20–25%) and, hence, reproduced the human pathologic condition of renal hypoplasia. This phenotype resulted from depletion of c‐myc–positive cells in cap mesenchyme, causing a ∼35% marked decrease of Six2‐ and Cited1‐stem/progenitor population and of proliferation that likely impaired self‐renewal. By contrast, c‐myc loss from e17.5 onward had no impact on late renal differentiation/maturation and/or homeostasis, providing evidence that c‐myc is dispensable during these phases. This study identified c‐myc as a modulator of renal organogenesis through regulation of stem/progenitor cell population. Developmental Dynamics 238:405–414, 2009. © 2009 Wiley‐Liss, Inc.