bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Assessment of Online Written Health Information on Treatment of Erectile Dysfunction in English and Hindi Language Webpages
Authors: Mathur Rahul , Singh Swarndeep , Kumar Saurabh , Kukreja Sarthak , Sharma Pawan 
Published: March 2024

Objectives: To assess and compare the overall quality of online written information on the treatment of erectile dysfunction (ED) in English and Hindi language webpages. Also, correlates of the quality of online health information were explored. Methods: First 50 links obtained on Google search in both English and Hindi language were screened, and a total of 86 (45 English, 41 Hindi) webpages providing patient-oriented information on the treatment of ED were analysed. Content quality and reliability were assessed using the DISCERN scale and the JAMA benchmark criteria respectively. Additionally, aesthetics, interactivity, and readability were evaluated. Results: The DISCERN score indicative of content quality was significantly higher for English webpages compared to Hindi webpages. English webpages were more likely to meet disclosure and attribution JAMA criteria for the reliability of medical information. English webpages with HONcode certification were more likely to have better content quality than those without it. However, none of the Hindi web pages analyzed were HONcode certified. Conclusion: English language webpages provided better overall quality of information on treatment of ED as compared to Hindi language. HONcode label might be used as a proxy indicator of better content quality for online resources informing on ED treatment by laypersons. There is a need to develop and popularize the usage of HONcode or other similar online health information quality accreditation systems for webpages in non-English languages like Hindi.
The Impact of Radiation Therapy on Sexual Health in Breast, Cervix, and Prostate Cancer Patients: A Systematic Review
Authors: Ballal Yashi , Aghaei Novin , Shaik Mohammed Shafiulla , Arora Sanjana , Khan Mahrukh , Vachon Tyler 
Published: March 2024

Purpose: To examine the effects of radiation therapy on sexual health in cervical, breast, and prostate cancer patients. Methods: Four electronic databases (PubMed/PubMed Central, Google Scholar, Embase(Ovid), and SCOPUS) were searched, and articles published in English from January 1, 2011, through 2022 were extracted. Five reviewers independently selected primary studies that reported patient sexual health symptoms caused by cervical, breast, or prostate cancer following radiation therapy treatment. The primary outcome measures sexual health trends during or after radiation therapy through external beam radiotherapy or brachytherapy where applicable. Secondary outcomes measured predictive factors contributing to sexual health scores (age, race, and geographical location). Results: 32 studies with a total of 5033 individuals were included in the systematic review. Overall, 19 studies reported a reduction in sexual health trends, 8 reported mixed trends, and 4 reported no significant trends in sexual health. Of the 10 breast cancer studies, 6 reported decreased sexual well-being, 2 found no trends, and 2 stated mixed trends. Among 12 prostate cancer studies, 5 reported worsening sexual health, 2 found no trends, 1 reported positive sexual functioning, and 4 found mixed trends. Among 10 cervical cancer studies, 8 reported worse sexual health, while 2 reported mixed trends. Younger breast and cervical cancer patients who received radiation therapy experienced greater body image and sexual health concerns. Evidence was insufficient to evaluate trends in geographical location or race between sexual health and radiation therapy. Conclusion: In this study, prostate, breast, and cervical cancer patients who received radiation therapy treatment experienced more troublesome sexual health symptoms compared to non-irradiated cancer patients. Interventions to improve sexual health should be prioritized.
The experience of traumatic events, psychological distress, and social support: links to COVID-19 vaccine hesitancy and trends with age in a group of older Australians
Authors: Christou-Ergos Maria , Wiley Kerrie E. , Leask Julie 
Published: March 2024

Abstract
Background
Vaccination is important to reduce disease-associated morbidity and mortality in an ageing global population. While older adults are more likely than younger adults to accept vaccines, some remain hesitant. We sought to understand how traumatic events, psychological distress and social support contribute to older adults’ intention to receive a COVID-19 vaccine and whether these experiences change with age.

Methods
We analysed survey data collected as part of the Sax Institute’s 45 and Up Study in a population of Australian adults aged 60 years and over. Data were derived from the COVID Insights study; a series of supplementary surveys about how participants experienced the COVID-19 pandemic.

Results
Higher intention to receive a COVID-19 vaccine was associated with greater social support (adjusted odds ratio (aOR):1.08; 95%CI:1.06–1.11; p <.001) while lower intention was associated with personally experiencing a serious illness, injury or assault in the last 12 months (aOR:0.79; 95% CI:0.64–0.98; p =.03). Social support and the experience of traumatic events increased significantly with age, while psychological distress decreased.

Conclusions
There may be factors beyond disease-associated risks that play a role in vaccine acceptance with age. Older Australians on the younger end of the age spectrum may have specific needs to address their hesitancy that may be overlooked.
MicroRNA expression as a prognostic biomarker of tongue squamous cell carcinoma (TSCC): a systematic review and meta-analysis
Authors: Sun Yiwei , Li Yuxiao , Zhou Wenjuan , Liu Zhonghao 
Published: March 2024

Abstract
Background
Recent studies have indicated that microRNA (miRNA) expression in tumour tissues has prognostic significance in Tongue squamous cell carcinoma (TSCC) patients. This study explored the possible prognostic value of miRNAs for TSCC based on published research.

Methods
A comprehensive literature search of multiple databases was conducted according to predefined eligibility criteria. Data were extracted from the included studies by two researchers, and HR results were determined based on Kaplan‒Meier curves according to the Tierney method. The Newcastle‒Ottawa Scale (NOS) and GRADE (Grading of Recommendations Assessment, Development, and Evaluation) pro-GDT were applied to assess the quality of all studies. Publication bias was estimated by funnel plot, Egger’s rank correlation test and sensitivity analysis.

Results
Eleven studies (891patients) were included, of which 6 reported up-regulated miRNAs and 7 mentioned down-regulated miRNAs. The pooled hazard ratio (HR) from the prognostic indicator overall survival (OS) was 1.34 (1.25–1.44), p < 0.00001, indicating a significant difference in miRNA expression between TSCC patients with better or worse prognosis.

Conclusion
MiRNAs may have high prognostic value and could be used as prognostic biomarkers of TSCC.
Very low-calorie ketogenic diet (VLCKD): a therapeutic nutritional tool for acne?
Authors: Verde Ludovica , Frias-Toral Evelyn , Cacciapuoti Sara , Simancas-Racines Daniel , Megna Matteo , Caiazzo Giuseppina , Potestio Luca , Maisto Maria , Tenore Gian Carlo , Colao Annamaria , Savastano Silvia , Muscogiuri Giovanna , Barrea Luigi 
Published: March 2024

Abstract
Background
Acne, a chronic inflammatory disease impacting the pilosebaceous unit, is influenced significantly by inflammation and oxidative stress, and is commonly associated with obesity. Similarly, obesity is also associated with increased inflammation and oxidation. The role of diet in acne remains inconclusive, but the very low-calorie ketogenic diet (VLCKD), known for weight loss and generating anti-inflammatory ketone bodies, presents promising potential. Despite this, the effects of VLCKD on acne remain underexplored. This study aimed to investigate the efficacy of a 45-day active phase of VLCKD in reducing the clinical severity of acne in young women with treatment-naïve moderate acne and grade I obesity.

Methods
Thirty-one women with treatment-naïve moderate acne, grade I obesity (BMI 30.03–34.65 kg/m2), aged 18–30 years, meeting inclusion/exclusion criteria, and consenting to adhere to VLCKD were recruited. Baseline and post-intervention assessments included anthropometric measurements, body composition, phase angle (PhA), trimethylamine N-oxide (TMAO) levels, and reactive oxygen metabolite derivatives (dROMs) as markers of inflammation, dysbiosis, and oxidative stress, respectively. A comprehensive dermatological examination, incorporating the Global Acne Grading System (GAGS) and the Dermatology Life Quality Index (DLQI), was conducted for all women.

Results
VLCKD resulted in general improvements in anthropometric and body composition parameters. Significantly, there were significant reductions in both the GAGS score (Δ%: − 31.46 ± 9.53, p < 0.001) and the DLQI score (Δ%: − 45.44 ± 24.02, p < 0.001) after the intervention. These improvements coincided with significant decreases in TMAO (p < 0.001) and dROMs (p < 0.001) levels and a significant increase in PhA (Δ%: + 8.60 ± 7.40, p < 0.001). Changes in the GAGS score positively correlated with changes in dROMs (p < 0.001) and negatively with PhA (p < 0.001) even after adjusting for Δ% FM. Changes in the DLQI score positively correlated with changes in dROMs (p < 0.001) and negatively with PhA (p < 0.001) even after adjustment for Δ% FM.

Conclusion
Given the side effects of drugs used for acne, there is an increasing need for safe, tolerable, and low-cost treatments that can be used for acne disease. The 45-day active phase of VLCKD demonstrated notable improvements in acne severity, and these improvements seemed to be attributable to the known antioxidant and anti-inflammatory effects of VLCKD.

Graphical Abstract
Introduction of protein vaccine candidate based on AP65, AP33, and α-actinin proteins against Trichomonas vaginalis parasite: an immunoinformatics design
Authors: Ghasemi Nezhad Forozan , Karmostaji Afsaneh , Sarkoohi Parisa , Shahbazi Behzad , Gharibi Zahra , Negahdari Batul , Ahmadi Khadijeh 
Published: March 2024

Abstract
Background
Trichomonas vaginalis is the most common nonviral sexually transmitted disease (STI) worldwide. Vaccination is generally considered to be one of the most effective methods of preventing infectious diseases. Using AP65, AP33 and α-actinin proteins, this research aims to develop a protein vaccine against Trichomonas vaginalis.

Methods
Based on the B-cell and T-cell epitope prediction servers, the most antigenic epitopes were selected, and with the necessary evaluations, epitope-rich domains of three proteins, AP65, AP33, and α-actinin, were selected and linked. Subsequently, the ability of the vaccine to interact with toll-like receptors 2 and 4 (TLR2 and TLR4) was assessed. The stability of the interactions was also studied by molecular dynamics for a duration of 100 nanoseconds.

Results
The designed protein consists of 780 amino acids with a molecular weight of 85247.31 daltons. The results of the interaction of the vaccine candidate with TLR2 and TLR4 of the immune system also showed that there are strong interactions between the vaccine candidate protein with TLR2 (-890.7 kcal mol-1) and TLR4 (-967.3 kcal mol-1). All parameters studied to evaluate the stability of the protein structure and the protein-TLR2 and protein-TLR4 complexes showed that the structure of the vaccine candidate protein is stable alone and in complex with the immune system receptors. Investigation of the ability of the designed protein to induce an immune response using the C-ImmSim web server also showed that the designed protein is capable of stimulating B- and T-cell lymphocytes to produce the necessary cytokines and antibodies against Trichomonas vaginalis.

Conclusions
Overall, our vaccine may have potential protection against Trichomonas vaginalis. However, for experimental in vivo and in vitro studies, it may be a good vaccine candidate.

Graphical Abstract
Post-radiation lichen planus: a case report and review of the literature
Authors: Musick Adam N. , Kim Do Young , Baumrucker Steven J. 
Published: March 2024

Abstract
Background
Lichen planus is a T-cell mediated inflammatory disorder of the skin and mucus membranes and is a rare complication of external beam radiation.

Case presentation
64 year old White male who presented to dermatology with a lesion at the lateral aspect of the right thigh. The lesion was first noted 40 years prior and had grown from 1.5 cm to 6.5 cm in the ensuing years. On examination the lesion was raised, hypopigmented, with pearly borders and central ulceration. Wide excision with lymph node dissection demonstrated invasive squamous cell carcinoma, basaloid type, with negative margins. Patient had radiation therapy of the right inguinal nodes. Patient subsequently noted a “blister” on the right upper thigh, which progressed over time to flat, polygonal, intensely pruritic lesions that covered the right lateral thigh from just distal to the hip to the distal third of the femur (Figs. 1, 2). Skin biopsy was positive for lichen planus. He was started on topical triamcinolone with salutary effects on appearance and pruritus.

Conclusion
Once more biopsy-proven cases of post-radiation lichen planus are reported, hopefully the exact mechanism can be elucidated. This may identify risk factors and aid in treatment, with the goal of limiting radiation toxicity and palliation of symptoms that may affect the quality of daily life.
Atypical lipoma of the right piriformis muscle: a case report and review of the literature
Authors: Qiu Xiao , Luo Xiaoyong , Wu Renmei 
Published: March 2024

Abstract
Background
Piriformis muscle mass is rare, which is particular for intrapiriformis lipoma. Thus far, only 11 cases of piriformis muscle mass have been reported in the English literature. Herein, we encountered one patient with intrapiriformis lipoma who was initially misdiagnosed.

Case presentation
The patient is a 50-year-old Chinese man. He complained of osphyalgia, right buttock pain, and radiating pain from the right buttock to the back of the right leg. Both ultrasound and magnetic resonance imaging demonstrated a cyst-like mass in the right piriformis muscle. Ultrasonography-guided aspiration was performed on this patient first, but failed. He was then recommended to undergo mass resection and neurolysis of sciatic nerve. Surprisingly, final histology revealed the diagnosis of intrapiriformis lipoma. The patient exhibited significant relief of symptoms 3 days post-surgery.

Conclusion
Diagnosis and differential diagnosis of radicular pain are potentially challenging but necessary. Atypical lipoma is prone to be misdiagnosed, especially in rare sites. It is notable for clinicians to be aware of the presence of intrapiriformis lipoma to avoid misdiagnosis and inappropriate treatment.
FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury
Authors: Zhou Mi , Liu Yang-Wu-Yue , He Yu-Hang , Zhang Jing-Yu , Guo Hao , Wang Hao , Ren Jia-Kui , Su Yi-Xun , Yang Teng , Li Jia-Bo , He Wen-Hui , Ma Peng-Jiao , Mi Man-Tian , Dai Shuang-Shuang 
Published: March 2024

Abstract
Background
Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury (TBI). However, the heterogeneity, multifunctionality, and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly understood.

Methods
Using the combined single-cell transcriptomics, metabolomics, and proteomics analysis from TBI patients and the TBI mouse model, we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral tests. We also characterized the underlying mechanisms both in vitro and in vivo through molecular simulations, signaling detections, gene expression regulation assessments [including dual-luciferase reporter and chromatin immunoprecipitation (ChIP) assays], primary cultures or co-cultures of neutrophils and oligodendrocytes, intracellular iron, and lipid hydroperoxide concentration measurements, as well as forkhead box protein O1 (FOXO1) conditional knockout mice.

Results
We identified that high expression of the FOXO1 protein was induced in neutrophils after TBI both in TBI patients and the TBI mouse model. Infiltration of these FOXO1high neutrophils in the brain was detected not only in the acute phase but also in the chronic phase post-TBI, aggravating acute brain inflammatory damage and promoting late TBI-induced depression. In the acute stage, FOXO1 upregulated cytoplasmic Versican (VCAN) to interact with the apoptosis regulator B-cell lymphoma-2 (BCL-2)-associated X protein (BAX), suppressing the mitochondrial translocation of BAX, which mediated the antiapoptotic effect companied with enhancing interleukin-6 (IL-6) production of FOXO1high neutrophils. In the chronic stage, the “FOXO1-transferrin receptor (TFRC)” mechanism contributes to FOXO1high neutrophil ferroptosis, disturbing the iron homeostasis of oligodendrocytes and inducing a reduction in myelin basic protein, which contributes to the progression of late depression after TBI.

Conclusions
FOXO1high neutrophils represent a novel neutrophil phenotype that emerges in response to acute and chronic TBI, which provides insight into the heterogeneity, reprogramming activity, and versatility of neutrophils in TBI.
Dispersion of antimicrobial resistant bacteria in pig farms and in the surrounding environment
Authors: Scicchitano Daniel , Leuzzi Daniela , Babbi Giulia , Palladino Giorgia , Turroni Silvia , Laczny Cédric Christian , Wilmes Paul , Correa Federico , Leekitcharoenphon Pimlapas , Savojardo Castrense , Luise Diana , Martelli Pierluigi , Trevisi Paolo , Aarestrup Frank Møller , Candela Marco , Rampelli Simone 
Published: March 2024

Abstract
Background
Antimicrobial resistance has been identified as a major threat to global health. The pig food chain is considered an important source of antimicrobial resistance genes (ARGs). However, there is still a lack of knowledge on the dispersion of ARGs in pig production system, including the external environment.

Results
In the present study, we longitudinally followed one swine farm located in Italy from the weaning phase to the slaughterhouse to comprehensively assess the diversity of ARGs, their diffusion, and the bacteria associated with them. We obtained shotgun metagenomic sequences from 294 samples, including pig feces, farm environment, soil around the farm, wastewater, and slaughterhouse environment. We identified a total of 530 species-level genome bins (SGBs), which allowed us to assess the dispersion of microorganisms and their associated ARGs in the farm system. We identified 309 SGBs being shared between the animals gut microbiome, the internal and external farm environments. Specifically, these SGBs were characterized by a diverse and complex resistome, with ARGs active against 18 different classes of antibiotic compounds, well matching antibiotic use in the pig food chain in Europe.

Conclusions
Collectively, our results highlight the urgency to implement more effective countermeasures to limit the dispersion of ARGs in the pig food systems and the relevance of metagenomics-based approaches to monitor the spread of ARGs for the safety of the farm working environment and the surrounding ecosystems.
Psychophysiological Responses to High-Intensity Interval Training Exercise over Menstrual Cycle Phases: An Exploratory Study
Authors: CHAVAN MANSI , PRADO RAUL COSME RAMOS , ASHLEY CANDI D. , KILPATRICK MARCUS W. 
Published: March 2024

ABSTRACT

Introduction
Although there is potential for the menstrual cycle to impact psychophysiological responses to exercise, there has been a paucity of studies investigating this aspect, specifically in the context of high-intensity interval training (HIIT).

Purpose
This exploratory study aimed to examine the psychophysiological responses to HIIT over the menstrual cycle.

Methods
Twenty-three healthy and physically active young women were recruited. Each woman completed a cycle ergometer HIIT session in the menstrual, follicular, and luteal phases of the menstrual cycle in a random order. Psychophysiological variables of interest were collected at baseline, and during and after exercise. Primary variables of interest were anxiety, mood, motivation, enjoyment, arousal, affect, and menstrual distress.

Results
Higher pain, water retention, behavior change (related to social interactions, eating habits, and self-performance), and autonomic reactions (consisting of nausea, dizziness, hot flashes, and cold sweats) were observed before HIIT during the menstrual phase compared with the follicular phase (P < 0.05). Participants exhibited worse psychological responses to HIIT sessions during the menstrual phase, followed by the luteal phase. Findings also noted participants arrived to the laboratory with lower motivation to initiate HIIT sessions during the luteal and menstrual phases compared with the follicular phase. This difference was observed before and during HIIT. Motivation and depressive symptoms showed a significant reduction from pre-HIIT to post-HIIT (P < 0.05), regardless of menstrual phase.

Conclusions
The findings revealed that the menstrual cycle had a significant impact on psychophysiological responses. Moreover, HIIT could be adopted to improve motivation and depressive symptoms; however, further research is needed to explore the effects of HIIT across the menstrual cycle in women with clinical depression.
Monitoring the Connection Between the Application of EFQM Model Principles and the Results of Organisations
Authors: Briš Petr , Urbanek Tomas 
Published: March 2024

Purpose: The present paper evaluated the connections between the degree of fulfilment of the EFQM model's requirements and the organisation's size, key results, social responsibility, employee satisfaction and customer satisfaction.
Methodology/Approach: The dataset was acquired via an internet-based survey hosted on Google Forms. The survey targeted managerial personnel within manufacturing entities situated within the geographical boundaries of the Czech Republic.
Findings: Research on the relationship between the degree of fulfilment of the EFQM model requirements and the organisation's size, critical organisational results, social responsibility, employee satisfaction, customer satisfaction and overall organisational results demonstrated a moderately significant to strong correlation for all monitored indicators.
Research limitation/implication: The data is collected only in one specific country, but the production organisations in which the research was carried out are mainly foreign-owned.
Originality/Value of paper: The current research results offer practical and theoretical implications. Theoretically, the research expands the literature on the EFQM model and its connection with current trends, which are the higher innovative activity of organisations and correlation with Industry 4. Practically, the research results will benefit managers, directors, and leaders of companies by allowing them to maintain an optimal level of quality management and estimate future requirements for complex enterprise integration.