bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Real-World Analysis of Anti-Programmed Death-Ligand 1 (PD-L1) Immune Checkpoint Inhibitor Treatments Combined with Chemotherapy in Untreated Extensive Stage Small Cell Lung Cancer
Authors: Hsu Ping-Chih , Wu Bing-Chen , Wang Chin-Chou , Chiu Li-Chung , Chang Chiung-Hsin , Liu Ping-Chi , Wu Chiao-En , Kuo Scott Chih-Hsi , Ju Jia-Shiuan , Huang Allen Chung-Cheng , Lin Yu-Ching , Yang Cheng-Ta , Ko How-Wen 
Published: February 2024

Abstract
Purpose: The real-world clinical experience of using anti-programmed death-ligand 1 (PD-L1) immune checkpoint inhibitors (ICIs) combined with chemotherapy in the first-line treatment of extensive-stage small cell lung cancer (SCLC) patients has rarely been reported. In this study, we aimed to perform a retrospective multicenter clinical analysis of extensive-stage SCLC patients receiving first-line therapy with anti-PD-L1 ICIs combined with chemotherapy.
Methods: Between November 2018 and March 2022, 72 extensive-stage SCLC patients receiving first-line atezolizumab or durvalumab in combination with chemotherapy according to the cancer center database of Linkou, Chiayi, and Kaohsiung Chang Gung Memorial Hospitals were retrospectively included in the analysis.
Results: Twenty-one patients (29.2%) received atezolizumab, and 51 (70.8%) received durvalumab. Objective response (OR) and disease control (DC) rates of 59.7% and 73.6% were observed with first-line ICI plus chemotherapy. The median progression-free survival (PFS) was 6.63 months (95% confidence interval (CI), 5.25–8.02), and the median overall survival (OS) was 16.07 months (95% CI, 15.12–17.0) in all study patients. A high neutrophil-to-lymphocyte ratio (NLR) (>4) and a high serum lactate dehydrogenase (LDH) concentration (>260 UL) were identified as independent unfavorable factors associated with shorter OS in multivariate analysis. Regarding safety, neutropenia was the most common grade 3 treatment-related adverse event (AE), but no treatment-related deaths occurred in the study patients.
Conclusion: First-line anti-PD-L1 ICIs combined with chemotherapy are effective and safe for extensive-stage SCLC. Further therapeutic strategies may need to be developed for patients with unfavorable outcomes (baseline high NLR and serum LDH level).
Development Patterns and Research Hotspots in Synovial Membrane Mesenchymal Stem Cells and Synovial Fluid Mesenchymal Stem Cells
Authors: Xu Qi , Wang Sheng , Fan Peixin , Hou Wenrun , Wang Lei , Gao Jinfang 
Published: February 2024

Abstract

Synovial membrane mesenchymal stem cells (SM-MSCs), a type of tissue-specific stem cells, are involved in forming synovial joints in embryos and the maintenance and repair of joint tissue in adults. Mesenchymal stem cells (MSCs) produced from synovial fluid (SF-MSCs) may have therapeutic properties with a higher capacity for cartilage proliferation than other MSC types.The research domains of SM-MSCs and SF-MSCs, however, have not been subjected to bibliometric analysis.This study aimed to visualize and analyze their research areas and trends using a bibliometric approach. Most studies on SM-MSCs and SF-MSCs are focused on basic research, but as SM-MSCs play important roles in chondrogenesis, osteogenesis, myogenesis, and tendon formation, they may have therapeutic potential for cartilage and tissue repair linked to diseases. The current study is the first to thoroughly introduce the development patterns and hotspots in this field of study.
Dynamic compensation of cerebellum and sensorimotor network in tremor dominated Parkinson’s disease
Authors: Shen Bo , Yao Qun , Li Wei , Wang Yaxi , Jiang Yinyin , Jiang Xu , Zhao Yang , Li Dongfeng , Chen Yaning , Zhu Jun , Yan Jun , Pan Yang , Zhang Wenbin , Zhu Qi , Zhang Daoqiang , Zhang Li , Wu Yuncheng 
Published: February 2024

Abstract
Aim: Parkinson's disease(PD) tremor was the symptom that associated basal ganglia(BG), cerebellum(CB), and sensorimotor(SMN) networks, we investigated the tremor-related static functional network connectivity(SFNC) and dynamic functional network connectivity(DFNC).
Methods: We analyzed the resting-state functional MRI data of 21 tremor-dominant Parkinson's disease (TDPD) patients and 29 healthy controls (HC). Then we compared the DFNC and SFNC of the three networks and examined their associations with tremor symptoms.
Results: In TDPD patients, the SFNC between SMN and BG networks was increased. Patients with TDPD spent more mean dwell time (MDT) in state 2 indicating sparse connections and less MDT in state 4 indicating stronger connections. DFNC between the cerebellum and SMN in state 2 was enhanced. The MDT of state 2 was positively associated with tremor scores.
Conclusion: The increased connection between the cerebellum and sensorimotor region was a result of dynamic compensation, TDPD patients were more likely to remain in the condition of sparse connection.
Preserving Fertility: Minimizing Ovarian Lymphatic Drainage Disruption in Gynecological Cancer Surgeries
Authors: Chen Dan , Wu Chuqing , Wei Simin , Guo Yican , Tang Weicheng , Xue Liru , Zhou Su , Fu Fangfang , Xiong Jiaqiang , Zhang Jinjin , Dai Jun , Li Yan , Wang Wenwen , Wu Meng , Wang Shixuan 
Published: February 2024

Abstract
Background
In systematic lymphadenectomy surgeries for gynecological malignancies, the potential disruption of ovarian lymphatic drainage function is a concern, particularly in individuals with a vested interest in fertility preservation. However, the current impact of lymphatic drainage disruption on ovarian function remains unclear and has not received sufficient attention from clinical practitioners. This study aims to elucidate the effects of ovarian lymphatic drainage obstruction on ovarian function in mice.
Methods
Evans blue dye was injected into the ovaries of mice to visualize ovarian draining lymphatic vessels (dLVs), which were subsequently ligated under the stereomicroscope, aiming to replicate the surgical injury to the ovarian lymphatic drainage during systematic lymphadenectomy. Serum estradiol, progesterone and anti-Müllerian hormone (AMH) levels were detected by ELISA, the number of follicles was counted by H&E staining, pregnancy rate and average litter sizes were evaluated through mating test, and the oocyte quality was assessed via spindle staining, ROS level detection, and Annexin-V staining. Additionally, immune cells and cytokines in the ovary were analyzed.
Results
Following intraovarian injection of Evans blue dye, the lymphatic vessels near the porta ovarii were blue, subsequently draining into the draining lymphatic nodes (dLNs) in the dorsal region of the upper pole of the kidney. At 30 days post dLV ligation, the ligation group exhibited a significant increase in ovarian weight and ovarian index compared to the control group. Obstruction of ovarian lymphatic drainage led to the decrease of serum progesterone and AMH levels by nearly 50%, the decrease of growing follicles and the increase of atretic follicles. The pregnancy rate decreased from 81.9–29.8% and the average litter size decreased from 5.6 to 3.3 per female in the ligation group. Furthermore, obstruction of ovarian lymphatic drainage elevated oxidative stress levels in oocytes, promoted oocyte apoptosis and meiosis arrest. These outcomes of impaired ovarian function may be attributed to the disturbance of the ovarian immune microenvironment, characterized by an increase of macrophages and the proinflammatory factors IL-6 in mouse ovaries.
Conclusions
The obstruction of ovarian lymphatic drainage leads to ovarian dysfunction, suggesting the importance of minimizing disruption to the ovarian lymphatic drainage pathway during lymphadenectomy, particularly in cases involving fertility preservation management.
Use of Sweet Potato as a Component in the Development of Laboratory Medium for the Cultivation of Lactobacillus.sp
Authors: Eddin Abdulhakim Sharaf , Ibrahim Salam A. 
Published: February 2024

Abstract

Due to its abundance, low cost and sustainability, sweet potato, a widely cultivated tuberous root rich in carbohydrates and essential nutrients, presents an intriguing prospect as a potential growth medium for Lactobacillus.sp. In the present study, we investigated the feasibility and efficacy of sweet potato-based medium for supporting the growth of Lactobacillus.sp bacteria. The sweet potato medium was supplemented with 2, 8, or 14 g/L of plant- based nitrogen sources (X-Seed Nucleo Max, X-Seed KAT, and NuCel 780 MG) to form SPM1, SPM2, and SPM3 respectively. Seven Lactobacillus.sp strains were used in this study, and a Lactobacilli MRS growth medium was used as a control. The growth pattern of tested Lactobacillus.sp strains grown in SPM2 and SPM3 was similar or higher than that in MRS. The population of Lactobacillus.sp strains reached an average of 7.94 ± 0.11, 8.38 ± 0.11, and 8.57 ± 0.12 at 12 h of incubation for MRS, SPM2, and SPM3, respectively. The buffering capacity of SPM2 and SPM3 was significantly (p < 0.05) higher than that in MRS, and the growth in SPM1 was lower than that of MRS. These findings demonstrate that SPM2 is suitable for the growth of Lactobacillus.sp strains and could be used as an alternative medium.
Flow birefringence of cellulose nanocrystal suspensions in three-dimensional flow fields: revisiting the stress-optic law
Authors: Nakamine Kento , Yokoyama Yuto , Worby William Kai Alexander , Muto Masakazu , Tagawa Yoshiyuki 
Published: February 2024

Abstract

This study systematically investigates the flow birefringence of cellulose nanocrystal (CNC) suspensions. The aim is to clarify the importance of the stress component along the camera's optical axis in the stress-optic law (SOL), which describes the relationship between birefringence, the retardation of transmitted polarized light, and the stress field. More than 100 datasets pertaining to the retardation of CNC suspensions (concentrations of 0.1, 0.3, 0.5, and 1.0 wt\%) in a laminar flow field within a rectangular channel (aspect ratios of 0.1, 1, and 3) are systematically obtained. The measured retardation data are compared with the predictions given by the conventional SOL excluding the stress component along the camera's optical axis and by the SOL including these components as second-order terms (2nd-order SOL). The results show that the 2nd-order SOL gives a significantly better agreement with the measurements. Based on the 2nd-order SOL, the retardation at the center of the channel, where the effect of the stress component along the camera's optical axis is most pronounced, is predicted to be proportional to the square of the flow rate, which agrees with the experimental data. The results confirm the importance of considering the stress component along the camera's optical axis in the flow birefringence of CNC suspensions at high flow rates, even for quasi-two-dimensional channel flow.
Fibroblast growth factor 23 induces myogenic differentiation of mouse muscle satellite cells in an FGF receptor 4 dependent manner
Authors: Wei Wen , Kang Qianqian , Cao Hui , Liu Shuling , Lin Ruiyu , Liu Hui , Chen Hangju , Cao Baozhen , Tu Mei 
Published: February 2024

Abstract
Fibroblast growth factor 23 (FGF23) is known for regulating phosphate and vitamin D metabolism, but its function in muscle satellite cells and skeletal muscle is unclear yet. Our study explores the role of muscle satellite cells and FGF23 in muscle development, and we specifically investigated the FGF23/FGFR4 signaling pathway in muscle satellite cells and its effect on myogenic differentiation, which is essential for the treatment of skeletal sarcopenia. We treated male C57BL/6J mice with hydrocortisone and d-galactose to establish a model of skeletal sarcopenia to observe the expression of FGF23, FGFR4, and other related proteins. The expression of myosin heavy chain (MHC), myogenic fiber differentiation (MyoD) and FGFR4 was up-regulated after transfection of muscle satellite cells with FGF23 overexpression vector and down-regulated after transfection of muscle satellite cells with FGF23 siRNA.The expression levels of MHC and MyoD were higher than those of the empty vector group after transfection of FGF23 siRNA and FGFR4 overexpression vector.Our findings suggest that FGF23 is a novel factor in regulating skeletal muscle mass, and the FGF23/FGFR4 signaling pathway plays a crucial role in the myogenic differentiation of muscle satellite cells. This finding offers a new potential therapeutic approach for the treatment of sarcopenia.
Investigation of Physical attributes of Highland coffee (Coffea arabica L.) Bean varieties drying on lath house
Authors: Belachew Mikru Tesfa , Mohammed Abrar Sualeh 
Published: February 2024

Abstract
Coffee is the world’s favorite beverage and the second most traded commodity worldwide after oil in the international market both in terms of volume and value. The objective of the study was to investigate physical attributes of coffee varieties using lath house drying. Samples of coffee varieties were prepared in wet processing method at harvesting of 2018/19 cropping season, grown in high land agro ecology part of south west, Ethiopia. So in this study, various mean values of physical attributes of selected coffee bean varieties with their standard deviation (Mean ± Sdv) were investigated at each measured moisture content (%wb). All most, all Physical properties of varieties such as Length (mm), Width (mm) and Thickness(mm),Geometric mean diameter(mm), Arithmetic mean diameter(mm),Equivalent mean diameter (mm), Frontal surface area (mm2), Sphericity(%), Aspect ratio(%), Shape index, Hundred bean weight (gm), Bulk density (kg/m3), Bean volume(mm3) and Angle of repose (degree) among varieties were no significant difference (P ≥ 0.05) except porosity and true density. Since the majority of the physical attributes of the selected varieties of coffee bean do not significantly differ, producers of coffee can generally utilize this option to lath house drying.
Causal role of immune cells in ovarian dysfunction :a mendelian randomization study
Authors: Weng Ruoxin , Ling Shuyi , Dai Yuqing , Li Yuan , Zhou Ziqiong , Wu Wenbo , Ren Chongyang , Zhang Liujuan , Wu Haifeng , Bai Mengying , Zhong Zhisheng , Zheng Yuehui 
Published: February 2024

Abstract

Background
At present, the etiology and mechanism of ovarian dysfunction are still unclear.Recent studies have indicated a potential correlation between immunity and ovarian dysfunction. However, the causal relationship between the immune cells and ovarian dysfunction still remains uncertain. For this aiticle,we aimed to figure out whether changes of immune cell composition contribute to ovarian dysfunction in this article.
Methods
Comprehensive two-sample Mendelian randomization analysis was performed to determine the causal role between immune cell compostitions and ovarian dysfunction in this study. The immune cell data are derived from the latest GWAS blood cell shape summary statistical data from the GWAS Catalog, and ovarian dysfunction data were obtained from the IEU Open GWAS. A total of 942 cases and 18,228 controls were included. A variety of analytical methods, including inverse variance weighting, weighted median, and MR-Egggera etc, were utilized to explore the link between immune cells and ovarian dysfunction. The Cochran's Q statistics were used to evaluate the heterogeneity of instrumental variables. The MR-Egger and MR pleiotropic residuals and outlier tests were utilized to detect the horizontal pleiotropy. The funnel plots and scatter plots visually assess heterogeneity and robustness.
Results
Our findings suggest that the presence of 36 immune phenotypes had a significant causal effect on ovarian dysfunction. Among them, 18 immunophenotypes were positively associated with ovarian dysfunction, including 7 in the B cell panel, 9 in the T cell panel, 1 in the monocyte cell panel and 1 in the NK cell panel; 28 immunophenotypes were negatively associated with ovarian dysfunction, including 11 in the B cell panel, 14 in the T cell panel, and in the monocyte cell panel.
Conclusion
Our study has demonstrated the close connection between immune cells and ovarian dysfunction by genetic background analysis. Further research is necessary to evaluate the potential of these immunophenotypes as early predictors of ovarian dysfunction, as well as possibility of new preventive strategies and new therapeutic targets.
Involvement of increased Arg-1+ILC2s and MDSCs in endometrial carcinoma: a pilot study
Authors: Gong Zhangyun , Lu Zhimin , Pan Anan , Wang Hao , Yi Haixing , Guo Peipei , Bi Kaihuan , Li Xuqing , Chen Ya , Xu Yuping , Cao Yunxia , Li Caihua , Jiang Huanhuan 
Published: February 2024

Abstract
Background
Endometrial carcinoma (EC) is a prevalent cancer in gynecology, and its survival rate is significantly low. Emerging studies have demonstrated that the combined action of group 2 innate lymphoid cell (ILC2) and myeloid-derived suppressor cell (MDSC) drives tumor progression.
Methods
A total of 41 EC patients and 40 controls were enrolled in this study. The ELISA kits were employed to measure the levels of IFN-γ, interleukin (IL)-4,22,25 and CCL3,4,5 in the serums; flow cytometry was used to characterize the populations of ILCs, MDSCs and arginase-1 (Arg-1) in both peripheral blood (PB) and carcinoma tissue (CT).
Results
We observed a significant elevation of Arg-1+ILC2 expression and a notable increase in MDSCs and Mo-MDSCs among PBMCs and CTs in the EC group. Additionally, the concentration of IL-25, a crucial cytokine in the ILC2-MDSC axis, was significantly higher in EC patients. Furthermore, a positive correlation between Arg-1+ILC2s and Mo-MDSCs was identified in EC patients. These findings suggest that elevated levels of Arg-1+ILC2s and Mo-MDSCs are associated with PFS.
Conclusion
In summary, Arg-1+ILC2s and Mo-MDSCs serve as a poor prognosis indicator of EC and they collectively participant in tumor promotion of EC.
Simulation-Optimization Model for the Structural Design of Cantilever Retaining walls
Authors: Shenouda Irini M. , Ali Momen 
Published: February 2024

Abstract

With the increasing trend of retaining wall construction in Egypt there is a need to reduce their construction costs. There are many types of retaining walls, cantilever, counterfort, buttressed and tied back walls. The common elements between all these types are the stem and the base. The cost of a retaining wall depends primarily on its material and secondarily on Excavation and backfill works. In this study, a new model is implemented in MATLAB to achieve the optimal design of the cantilever retaining wall elements. The design model is coupled with the shuffled complex evolution algorithm, developed at the University of Arizona (SCE-UA). This developed framework was applied to an existing model used by other researchers to demonstrate its efficiency and to obtain the most economical solution. The results proved that using the SCE-UA method provides superior results compared to those of other algorithms.
Machine learning approach identifies miRNA signatures for breast cancer detection and classification from patient urine samples
Authors: Maurer Jochen , Rübner Matthias , Kuo Chao-Chung , Klein Birgit , Franzen Julia , Wittenborn Julia , Kupec Tomas , Najjari Laila , Fasching Peter , Stickeler Elmar 
Published: February 2024

Abstract

Introduction
Breast cancer is the most common cancer in women, with one in eight women suffering from this disease in her lifetime. The implementation of centrally organized mammography screening for women between 50 and 69 years of age was a major step in the direction of early detection and lead to a significant improvement in cure rates. Within the screening program, women undergo a mammogram every two years with an implemented centralized quality-controlled review process. However, the participation rate reached only approximately 50% of the eligible women. In addition to several others, the technical aspects of mammography, including painful compression of the breast, are cited as a reason for not participating in this very important program. Therefore, focusing current research on less painful and less invasive techniques for the detection of breast cancer seems to be highly clinically useful. Liquid biopsies offer this option with distinct molecules or cells in line with the research. Blood-based tests of circulating tumor cells (CTCs), cell-free DNA (ctDNA) and cell-free miRNA have been performed in a variety of studies and tumor entities.
Methods
We performed miRNA sequencing on 82 urine samples, 32 samples from breast cancer patients (9× luminal A, 8× luminal B, 9× triple-negative and 6× HER2) and 50 healthy control samples. Data were analyzed and interpreted using Random Forest analysis.
Results
We identified a signature of 275 miRNAs that allows the detection of invasive breast cancer in urine from breast cancer patients. Furthermore, we identified distinct miRNA expression patterns for the major intrinsic subtypes of breast cancer, specifically luminal A, luminal B, HER2-enriched and triple-negative breast cancer.
Conclusions
Here, we present the first approach for sequencing miRNAs in female urine to detect breast cancer and, subsequently, intrinsic subtype-specific miRNA patterns. This experimental approach specifically validates miRNA sequencing as a technique for breast cancer detection in urine samples and opens the door to a new, easy and painless procedure for regular breast cancer screening.
Ginsenoside Rb1 attenuates doxorubicin induced cardiotoxicity by suppressing autophagy and Nrf2 induced ferroptosis
Authors: Zhai Yafei , Bai Jinmeng , Peng Ying , Cao Jinhua , Fang Guangming , Dong Yiming , Wang Ze , Lu Yanyu , Wang Mengyu , Liu Mengduan , Liu Yangyang , Li Xiaowei , Dong Jianzeng , Zhao Xiaoyan 
Published: February 2024

Abstract
Ginsenoside Rb1 (Rb1), an active component isolated from traditional Chinese medicine Ginseng, is beneficial to many cardiovascular diseases. However, whether it can protect against doxorubicin induced cardiotoxicity (DIC) is not clear yet. In this study, we aimed to investigate the role of Rb1 in DIC. Mice were injected with a single dose of doxorubicin (20mg/kg) to induce acute cardiotoxicity. Rb1 was given daily gavage to mice for 7 days. Changes in cardiac function, myocardium histopathology, oxidative stress, cardiomyocyte mitochondrion morphology as well as autophagy and ferroptosis pathway were studied to evaluate Rb1’s function on DIC. Meanwhile, RNA-seq analysis was performed to explore the potential underline molecular mechanism involved in Rb1’s function on DIC. We found that Rb1 treatment can improve survival rate and body weight in Dox treated mice group. Rb1 can attenuate Dox induced cardiac dysfunction and myocardium hypertrophy and interstitial fibrosis. The oxidative stress increase and cardiomyocyte mitochondrion destruction were improved by Rb1 treatment. Mechanism study found that Rb1’s beneficial role in DIC is through suppressing of autophagy and Nrf2 induced ferroptosis. This study shown that Ginsenoside Rb1 can protect against DIC by regulating autophagy and ferroptosis.