bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of nondegradable cyclins reveals distinct roles of the mitotic cyclins in Drosophila meiosis
Authors: Bourouh Mohammed , Dhaliwal Rajdeep , Rai Rajni , Qureshi Hafsah , Swan Andrew 
Published: March 2024

Abstract
Meiosis is a complex variant of the mitotic cell cycle, and as such relies on many of the same proteins involved in mitosis, but utilizes these in novel ways. As in mitosis, Cdk1 and its cyclin partners, Cyclin A, B, and B3 are required at multiple steps in meiosis. Here, we study the effect of stabilized forms of the three mitotic cyclins to study the consequences of failure to degrade the cyclins in meiosis. We find that stabilized Cyclin B3 promotes ectopic microtubule polymerization throughout the egg, dependent on APC/C activity and apparently due to the consequent destruction of Cyclin A and Cyclin B. We present data that suggests CycB, and possibly CycA, can also promote APC/C activity at specific stages of meiosis. We also present evidence that in meiosis APC/CCort and APC/CFzy are able to target Cyclin B via a novel degron. Overall, our findings highlight the distinct functions of the three mitotic Cdk–cyclin complexes in meiosis.
Sex peptide receptor is not required for refractoriness to remating or induction of egg laying in Aedes aegypti
Authors: Amaro I Alexandra , Wohl Margot P , Pitcher Sylvie , Alfonso-Parra Catalina , Avila Frank W , Paige Andrew S , Helinski Michelle E H , Duvall Laura B , Harrington Laura C , Wolfner Mariana F , McMeniman Conor J 
Published: March 2024

Abstract
Across diverse insect taxa, the behavior and physiology of females dramatically changes after mating—processes largely triggered by the transfer of seminal proteins from their mates. In the vinegar fly Drosophila melanogaster, the seminal protein sex peptide (SP) decreases the likelihood of female flies remating and causes additional behavioral and physiological changes that promote fertility including increasing egg production. Although SP is only found in the Drosophila genus, its receptor, sex peptide receptor (SPR), is the widely conserved myoinhibitory peptide (MIP) receptor. To test the functional role of SPR in mediating postmating responses in a non-Drosophila dipteran, we generated 2 independent Spr-knockout alleles in the yellow fever mosquito, Aedes aegypti. Although SPR is needed for postmating responses in Drosophila and the cotton bollworm Helicoverpa armigera, Spr mutant Ae. aegypti show completely normal postmating decreases in remating propensity and increases in egg laying. In addition, injection of synthetic SP or accessory gland homogenate from D. melanogaster into virgin female mosquitoes did not elicit these postmating responses. Our results demonstrate that Spr is not required for these canonical postmating responses in Ae. aegypti, indicating that other, as yet unknown, signaling pathways are likely responsible for these behavioral switches in this disease vector.
The Effects of the Built Environment on the Mental Health of Older Adults: A Case Study in Hangzhou, China
Authors: Kong Xinyu , Han Haoying , Zhan Mengyao , Chi Fangting 
Published: March 2024

Abstract

Background and Objectives
This study examines the association between the built environment and mental health among older adults by exploring the potential pathways through the perceived built environment, social interaction, and environmental pollution.

Research Design and Methods
Research data were collected from 1 405 older adults aged 60 years and older in 30 residential neighborhoods of Hangzhou, China, in 2022. The participants’ evaluation of the perceived built environment, social interaction, and environmental pollution were assessed. Mental health was measured by the 12-item Short-Form Health Survey. The built environment was measured through an evaluation of density, diversity, design, destination accessibility, distance to transit, and the age-friendly environment. Structural equation models were established based on 300-m, 500-m, and 800-m buffers from the entrance of residential neighborhoods. The propensity score method was employed for sensitivity analysis.

Results
With regard to older adult’s mental health, there were significant direct effects of the accessibility of health services, shopping services, and bus stops. A robust mediating effect of perceived built environment was displayed on the relationship between land use mix and mental health of older adults. Social interaction exhibited robust mediating effects between the age-friendly environment, the number of bus stops, and mental health of older adults.

Discussion and Implications
This study provides empirical experience for related research on the built environment and mental health as well as policy implications for urban planners focusing on community planning.
The Norepinephrine-QseC Axis Aggravates F. nucleatum-associated Colitis Through Interkingdom Signaling
Authors: Zhang Ling , Chen Guimei , Zeng Xianghao , Yue Huawen , Zheng Qiao , Hu Qing , Tian Qian , Liang Lanfan , Zhao Xinyu , Yang Ziyi , Bai Hang , Liu Yanqin , Zhao Ming , Fu Xiangsheng 
Published: March 2024

Abstract

Aims
Inflammatory bowel disease (IBD) is associated with F. nucleatum, and chronic stress can increase the risk of aggravation. However, whether norepinephrine (NE) can enhance the pathogenicity of F. nucleatum to aggravate dextran sulfate sodium salt (DSS)-induced colitis is unclear.

Methods
Transcriptome sequencing was used to identify differentially expressed genes in bacteria treated with NE. Affinity testing and molecular docking were applied to calculate and predict the binding of NE and Quorum sensing regulators C (QseC). The pathogenicity of Fusobacterium nucleatum treated with NE and QseC inhibitors was examined in vitro and further verified using the IBD mouse model induced by DSS.

Results
Norepinephrine could bind to QseC directly to upregulate the quorum sensing pathway of F. nucleatum and enhance its virulence gene expression (FadA, FomA, Fap2) and invasiveness in vitro. Meanwhile, it promoted the invasion of F. nucleatum into the intestine and increased the expression of host inflammatory cytokines (IL-6, IL-1β) to aggravate colonic inflammation in IBD mice. The QseC inhibitor LED209 inhibited the effect of NE on F. nucleatum and partially restored short-chain fatty acid (SCFA)–producing bacteria (Prevotellaceae, Lactobacillaceae) to attenuate colonic inflammation in IBD mice.

Conclusions
Generally, the NE-QseC axis enhanced the pathogenicity of F. nucleatum through interkingdom signaling to aggravate colonic inflammation in IBD mice. We see that QseC may be a potential target for microbiota management of IBD under chronic pressure.
Multi-objective two-stage optimization scheduling algorithm for virtual power plants considering low carbon
Authors: Wang Xuejin , Chen Chen , Shi Yao , Chen Qiang 
Published: March 2024

Abstract
With the continuous growth of energy demand, the importance of developing and utilizing low-carbon energy is becoming increasingly prominent. In this context, virtual power plant technology has been proposed. It is a technology for integrating, coordinating and optimizing distributed energy resources, which has significant effects in improving energy utilization efficiency and reducing carbon emissions. This article proposed a virtual power plant scheduling method based on multi-objective two-stage optimization scheduling algorithm considering low carbon. This method first determines the production and consumption of various energy resources in the virtual power plant, including wind power, thermal power and hydropower. Then, with the goal of minimizing costs and reducing carbon emissions, multi-objective optimization algorithms are used to allocate and schedule energy resources in the virtual power plant. On this basis, a two-stage optimization strategy was introduced, combining long-term optimization and short-term scheduling to adapt to energy allocation and scheduling needs at different time scales. The experimental results indicate that this method can effectively improve the energy utilization efficiency and economy of virtual power plants, and reduce carbon emissions.
Drug resistance and influencing factors in HIV-1-infected individuals under antiretroviral therapy in Guangxi, China
Authors: Pang Xianwu , He Qin , Tang Kailing , Huang Jinghua , Fang Ningye , Xie Haoming , Ma Jie , Zhu Qiuying , Lan Guanghua , Liang Shujia 
Published: March 2024

Abstract

Objectives
To assess the profiles and determinants of drug resistance in HIV-1-infected individuals undergoing ART in Guangxi.

Methods
Samples and data were collected from HIV-1-infected individuals experiencing virological failure post-ART from 14 cities in Guangxi. Sequencing of the HIV-1 pol gene was conducted, followed by analysis for drug resistance mutations using the Stanford University HIV Drug Resistance Database. Logistic regression was employed to identify potential risk factors associated with both HIV drug resistance and mortality.

Results
A total of 8963 individuals with pol sequences were included in this study. The overall prevalence of HIV-1 drug resistance (HIVDR) was 42.43% (3808/8963), showing a decrease from 59.62% to 41.40% from 2016 to 2023. Factors such as being aged ≥50 years, male, Han nationality, lower education levels, occupations including workers, peasants and children, AIDS, pre-treatment CD4 T cell counts &lt;200 cells/mm3, infection with CRF01_AE and CRF55_01B subtypes, and ART regimen lamivudine/zidovudine/nevirapine were associated with higher susceptibility to HIVDR. The common mutations were M184V (17.38%) and K103N (22.14%). Additionally, the prevalence of M184V, S68G, M41L and G190A were different between the Han and Zhuang populations. Factors including age, gender, ethnicity, education level, occupation, infectious route, clinical stage, viral load, subtype, ART regimen and HIVDR showed significant associations with mortality.

Conclusions
The factors contributing to drug resistance in the HIV-1 ART individuals in Guangxi appear to be notably intricate. Continuous reinforcement of drug resistance surveillance is imperative, accompanied by the optimization of ART regimens to mitigate virological failures effectively.
An Introduction to the Complete Blood Count for Clinical Chemists: Platelets
Authors: van Wijk Xander M R , Sanchez Oviol Zaraith , Winter William E , Harris Neil S , Marin Maximo J 
Published: March 2024

Abstract

Background
The most ordered laboratory test worldwide is the complete blood count (CBC).

Content
In this primer, an introduction to platelet testing in the context of the CBC is provided with a discussion of the laboratory evaluation of platelet abnormalities including thrombocytopenia and thrombocytosis.

Summary
As clinical chemists continue to be tasked to direct laboratories outside of the traditional clinical chemistry sections such as hematology, expertise must be developed. This primer is dedicated to that effort.
mRNA-Seq of testis and liver tissues reveals a testis-specific gene and alternative splicing associated with hybrid male sterility in dzo
Authors: Hong Rui , Wu Jiaxin , Chen Xining , Zhang Zhenghao , Liu Xinyue , Li Meichen , Zuo Fuyuan , Zhang Gong-Wei 
Published: March 2024

Abstract
Alternative splicing (AS) plays an important role in the co-transcription and post-transcriptional regulation of gene expression during mammalian spermatogenesis. The dzo is the male F1 offspring of an interspecific hybrid between a domestic bull (Bos taurus ♂) and a yak (Bos grunniens ♀) which exhibits male sterility. This study aimed to identify the testis-specific genes and AS associated with hybrid male sterility in dzo. The iDEP90 program and rMATS software were used to identify the differentially expressed genes (DEG) and differential alternative splicing genes (DSG) based on RNA-seq data from the liver (n = 9) and testis (n = 6) tissues of domestic cattle, yak, and dzo. Splicing factors (SF) were obtained from the AmiGO2 and the NCBI databases, and Pearson correlation analysis was performed on the differentially expressed SFs and DSGs. We focused on the testis-specific DEGs and DSGs between dzo and cattle and yak. Among the top 3,000 genes with the most significant variations between these 15 samples, a large number of genes showed testis-specific expression involved with spermatogenesis. Cluster analysis showed that the expression levels of these testis-specific genes were dysregulated during mitosis with a burst downregulation during the pachynema spermatocyte stage. The occurrence of AS events in the testis was about 2.5 fold greater than in the liver, with exon skipping being the major AS event (81.89% to 82.73%). A total of 74 DSGs were specifically expressed in the testis and were significantly enriched during meiosis I, synapsis, and in the piRNA biosynthesis pathways. Notably, STAG3 and DDX4 were of the exon skipping type, and DMC1 was a mutually exclusive exon. A total of 36 SFs were significantly different in dzo testis, compared with cattle and yak. DDX4, SUGP1, and EFTUD2 were potential SFs leading to abnormal AS of testis-specific genes in dzo. These results show that AS of testis-specific genes can affect synapsis and the piRNA biosynthetic processes in dzo, which may be important factors associated with hybrid male sterility in dzo.
Do Recessions and Bear Markets Occur Concurrently across Countries? A Multinomial Logistic Approach
Authors: Poon Aubrey , Zhu Dan 
Published: March 2024

Abstract
We introduce a novel multinomial logistic model for detecting and forecasting concurrent recessions and bear markets across multiple countries. Our framework leverages cross-country panel features and provides additional information for robust analysis. Through a comprehensive simulation study, we demonstrate the computational efficiency and accuracy of our model, even when handling multiple binary indicators. Applying our framework to empirical data from the United States, the UK, and Euro Area, we find that the multinomial logistic model produces superior medium-term forecasting of concurrent recession and bear market events across countries compared to multiple independent single logistic models. Additionally, our counterfactual analysis reveals that specific events, such as a recession and bear market in the United States, along with the tightening of financial conditions and a negative interest rate spread in the United States, increase the probability of concurrent and individual recession and bear market occurrences in the UK and Euro Area.
High-precision charge analysis in a catalytic nanoparticle by electron holography
Authors: Aso Ryotaro , Midoh Yoshihiro , Tanigaki Toshiaki , Murakami Yasukazu 
Published: March 2024

Abstract
The charge state of supported metal catalysts is the key to understand the elementary processes involved in catalytic reactions. However, high-precision charge analysis of the metal catalysts at the atomic level is experimentally challenging. To address this critical challenge, high-sensitivity electron holography has recently been successfully applied for precisely measuring the elementary charges on individual platinum nanoparticles supported on a titanium dioxide surface. In this review, we introduce the latest advancements in high-precision charge analysis and discuss the mechanisms of charge transfer at the metal–support interface. The development of charge measurements is entering a new era, and charge analyses under conditions closer to practical working environments, such as real-time, real-space, and reactive gas environments, are expected to be realized in the near future.
Broadcasted nonparametric tensor regression
Authors: Zhou Ya , Wong Raymond K W , He Kejun 
Published: March 2024

Abstract
We propose a novel use of a broadcasting operation, which distributes univariate functions to all entries of the tensor covariate, to model the nonlinearity in tensor regression nonparametrically. A penalized estimation and the corresponding algorithm are proposed. Our theoretical investigation, which allows the dimensions of the tensor covariate to diverge, indicates that the proposed estimation yields a desirable convergence rate. We also provide a minimax lower bound, which characterizes the optimality of the proposed estimator for a wide range of scenarios. Numerical experiments are conducted to confirm the theoretical findings, and they show that the proposed model has advantages over its existing linear counterparts.
Cloud-scale molecular gas properties of the ANTENNAE merger: a comparative study with PHANGS-ALMA galaxies and NGC 3256
Authors: Brunetti Nathan , Wilson Christine D , He Hao , Sun Jiayi , Leroy Adam K , Rosolowsky Erik , Bemis Ashley , Bigiel Frank , Groves Brent , Saito Toshiki , Schinnerer Eva 
Published: March 2024

ABSTRACT
We present observations of the central 9 kpc of the Antennae merger (NGC 4038/9) at 55 pc resolution in the CO (2–1) line obtained with the Atacama Large Millimeter/submillimeter Array (ALMA). We use a pixel-based analysis to compare the gas properties in the Antennae to those in 70 nearby spiral galaxies from the PHANGS-ALMA survey, as well as the merger and nearest luminous infrared galaxy NGC 3256. Compared to PHANGS-ALMA galaxies at matched spatial resolution, the molecular gas in the Antennae exhibits some of the highest surface densities, velocity dispersions, peak brightness temperatures, and turbulent pressures. However, the virial parameters in the Antennae are consistent with many of the PHANGS-ALMA galaxies. NGC 3256 has similar gas surface densities but higher nuclear velocity dispersions than the Antennae, as well as higher system-wide peak brightness temperatures and virial parameters. NGC 3256 is at a later stage in the merging process than the Antennae, which may result in more intense merger-driven gas flows that could drive up the turbulence in the gas. The high virial parameters in NGC 3256 may indicate that this increased turbulence is suppressing future star formation as NGC 3256 moves out of the starburst phase. In comparison, the relatively normal virial parameters in the Antennae may imply that it is about to undergo a new burst of star formation.
[Ga68] DOTATATE PET/MRI-guided radiosurgical treatment planning and response assessment in meningiomas
Authors: Ivanidze Jana , Chang Se Jung , Haghdel Arsalan , Kim Joon Tae , Roy Choudhury Arindam , Wu Alan , Ramakrishna Rohan , Schwartz Theodore H , Cisse Babacar , Stieg Philip , Muller Leland , Osborne Joseph R , Magge Rajiv S , Karakatsanis Nicolas A , Roytman Michelle , Lin Eaton , Pannullo Susan C , Palmer Joshua D , Knisely Jonathan P S 
Published: March 2024

Abstract

Background
Our purpose was to determine the utility of [68Ga]-DOTATATE PET/MRI in meningioma response assessment following radiosurgery.

Methods
Patients with meningioma prospectively underwent postoperative DOTATATE PET/MRI. Co-registered PET and gadolinium-enhanced T1-weighted MRI were employed for radiosurgery planning. Follow-up DOTATATE PET/MRI was performed at 6–12 months post-radiosurgery. Maximum absolute standardized uptake value (SUV) and SUV ratio (SUVRSSS) referencing superior sagittal sinus (SSS) blood pool were obtained. Size change was determined by Response Assessment in Neuro-Oncology (RANO) criteria. Association of SUVRSSS change magnitude and progression-free survival (PFS) was evaluated using Cox regression.

Results
Twenty-seven patients with 64 tumors (26% World Health Organization [WHO]-1, 41% WHO-2, 26% WHO-3, and 7% WHO-unknown) were prospectively followed post stereotactic radiosurgery (SRS) or stereotactic body radiotherapy (SBRT; mean dose: 30 Gy, modal dose 35 Gy, mean of 5 fractions). Post-irradiation SUV and SUVRSSS decreased by 37.4% and 44.4%, respectively (P &lt; .0001). Size product decreased by 8.9%, thus failing to reach the 25% significance threshold as determined by RANO guidelines. Mean follow-up time was 26 months (range: 6–44). Overall mean PFS was 83% and 100%/100%/54% in WHO-1/-2/-3 subcohorts, respectively, at 34 months. At maximum follow-up (42–44 months), PFS was 100%/83%/54% in WHO-1/-2/-3 subcohorts, respectively. Cox regression analyses revealed a hazard ratio of 0.48 for 10-unit reduction in SUVRSSS in the SRS cohort.

Conclusions
DOTATATE PET SUV and SUVRSSS demonstrated marked, significant decrease post-radiosurgery. Lesion size decrease was statistically significant; however, it was not clinically significant by RANO criteria. DOTATATE PET/MR thus represents a promising imaging biomarker for response assessment in meningiomas treated with radiosurgery.

ClinicalTrials.gov Identifier
NCT04081701
Individualized trajectories in postradiotherapy neurocognitive functioning of patients with brain metastases
Authors: van Grinsven Eva E , Cialdella Fia , Gmelich Meijling Yoniet , Verhoeff Joost J C , Philippens Marielle E P , van Zandvoort Martine J E 
Published: March 2024

Abstract

Background
The increasing incidence of brain metastases (BMs) and improved survival rates underscore the necessity to investigate the effects of treatments on individuals. The aim of this study was to evaluate the individual trajectories of subjective and objective cognitive performance after radiotherapy in patients with BMs.

Methods
The study population consisted of adult patients with BMs referred for radiotherapy. A semi-structured interview and comprehensive neurocognitive assessment (NCA) were used to assess both subjective and objective cognitive performance before, 3 months and ≥ 11 months after radiotherapy. Reliable change indices were used to identify individual, clinically meaningful changes.

Results
Thirty-six patients completed the 3-month follow-up, and 14 patients completed the ≥ 11-months follow-up. Depending on the domain, subjective cognitive decline was reported by 11–22% of patients. In total, 50% of patients reported subjective decline in at least one cognitive domain. Intracranial progression 3 months postradiotherapy was a risk-factor for self-reported deterioration (P = .031). Objective changes were observed across all domains, with a particular vulnerability for decline in memory at 3 months postradiotherapy. The majority of patients (81%) experienced both a deterioration as well as improvement (eg, mixed response) in objective cognitive functioning. Results were similar for the long-term follow-up (3 to ≥11 months). No risk factors for objective cognitive change 3 months postradiotherapy were identified.

Conclusions
Our study revealed that the majority of patients with BMs will show a mixed cognitive response following radiotherapy, reflecting the complex impact. This underscores the importance of patient-tailored NCAs 3 months postradiotherapy to guide optimal rehabilitation strategies.