bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analytical Method for Quantifying Monoterpenoids (p-Cymene and Myrcene) in Nanoemulsions Using High-Performance Liquid Chromatography
Authors: Lobato Duarte Jonatas , Delello Di Filippo Leonardo , Tavares Alberto Gomes , Chorilli Marlus 
Published: February 2024

Abstract

Background
Myrcene and cymene, aromatic monoterpenes found in plants and essential oils, possess distinctive aromatic qualities. However, their volatility and limited solubility pose challenges in precise handling and formulation. Meanwhile, nanoemulsions emerge as promising drug delivery systems, improving the bioavailability and stability of these active ingredients.

Objective
This article aimed to develop an HPLC method for the quantification of two monoterpenoids, p-cymene and myrcene, in nanoemulsions.

Method
The method used a Phenomenex® Synergi™ Fusion-RP column (150 mm × 4.6 mm id, 4 μm particle size) on an HPLC system with isocratic elution. The mobile phase was composed of acetonitrile and water (60:40, v/v) and was validated in terms of specificity, linearity, accuracy, precision, robustness, and selectivity.

Results
The method provided accurate and precise results with a correlation coefficient of 0.999 and RSD values of less than 2%. The method can be used for quality control of nanoemulsions containing these monoterpenoids and as a reference for future studies on their efficacy and stability.

Conclusions
The study demonstrates the feasibility of using HPLC for the quantification of monoterpenoids in nanoemulsions and its potential as a quality control tool for nanoemulsion-based drug delivery systems.

Highlights
The method’s accuracy, precision, and reliability, as evidenced by high correlation coefficients and low RSD values, underscore its suitability for ensuring the consistent formulation of these monoterpenoid-containing nanoemulsions, while also serving as a reference point for future research endeavors in this field.
Control effect and field application of four predatory Orius species on Megalurothrips usitatus (Thysanoptera: Thripidae)
Authors: Dai Xiaoyan , Wang Ruijuan , Liu Yan , Su Long , Yin Zhenjuan , Wu Mingfei , Chen Hao , Zheng Li , Zhai Yifan 
Published: February 2024

Abstract
Megalurothrips usitatus (Bagrall) is one of the most important pests of cowpea, Vigna unguiculata (Linn.) Walp in South China. Four Orius species, including Orius minutus (L.), Orius nagaii (Yasunaga), Orius sauteri (Poppius), and Orius strigicollis (Poppius), have been commercially produced and widely used as natural enemies of pests in China. In this study, we evaluated the control efficiency of these Orius species on M. usitatus in tropical Hainan Province, China, by recording the survival rates, developmental times, and predation effects in laboratory and semi-field conditions. Laboratory experiments showed that all these 4 Orius species preyed on M. usitatus under the experimental temperatures (25, 30, and 35 °C), and O. strigicollis exhibited the highest survival rate and predation effect. Semi-field cage experiments showed that the control effect of 4 Orius species on M. usitatus was significantly higher than that under normal chemical control, with O. strigicollis having the highest effect. Greenhouse experiments in Hainan Province, China, confirmed that O. strigicollis had a significant control effect on M. usitatus. Our study indicated that O. strigicollis has a significant potential for the control of M. usitatus in cowpea fields in southern China.
Noninvasive detection of Zika virus in mosquito excreta sampled from wild mosquito populations in French Guiana
Authors: Guidez Amandine , Fontaine Albin , Yousfi Léna , Moutailler Sara , Carinci Romuald , Issaly Jean , Gaborit Pascal , Cannet Arnaud , de Laval Franck , Matheus Séverine , Rousset Dominique , Dusfour Isabelle , Girod Romain , Briolant Sébastien 
Published: February 2024

Abstract
Arboviruses can be difficult to detect in the field due to relatively low prevalence in mosquito populations. The discovery that infected mosquitoes can release viruses in both their saliva and excreta gave rise to low-cost methods for the detection of arboviruses during entomological surveillance. We implemented both saliva and excreta-based entomological surveillance during the emergence of Zika virus (ZIKV) in French Guiana in 2016 by trapping mosquitoes around households of symptomatic cases with confirmed ZIKV infection. ZIKV was detected in mosquito excreta and not in mosquito saliva in 1 trap collection out of 85 (1.2%). One female Ae. aegypti L. (Diptera: Culicidae) was found with a ZIKV systemic infection in the corresponding trap. The lag time between symptom onset in a ZIKV-infected individual living near the trap site and ZIKV detection in this mosquito was 1 wk. These results highlight the potential of detection in excreta from trapped mosquitoes as a sensitive and cost-effective method to non invasively detect arbovirus circulation.
Rickettsia species identified in adult, host-seeking Dermacentor occidentalis (Acari: Ixodidae) from Baja California, Mexico, and Oregon and Washington, United States
Authors: Paddock Christopher D , Zambrano Maria L , Clover James R , Ladd-Wilson Stephen , Dykstra Elizabeth A , Salamone Amy , Kangiser David , Ayres Bryan N , Shooter Savannah L , Karpathy Sandor E , Kjemtrup Anne M , Beati Lorenza , Levin Michael L , Lane Robert S , Zazueta Oscar E 
Published: February 2024

Abstract
The Pacific Coast tick (Dermacentor occidentalis Marx, 1892) is a frequently encountered and commonly reported human-biting tick species that has been recorded from most of California and parts of southwestern Oregon, southcentral Washington, and northwestern Mexico. Although previous investigators have surveyed populations of D. occidentalis for the presence of Rickettsia species across several regions of California, populations of this tick have not been surveyed heretofore for rickettsiae from Baja California, Oregon, or Washington. We evaluated 1,367 host-seeking, D. occidentalis adults collected from 2015 to 2022 by flagging vegetation at multiple sites in Baja California, Mexico, and Oregon and Washington, United States, using genus- and species-specific assays for spotted fever group rickettsiae. DNA of Rickettsia 364D, R. bellii, and R. tillamookensis was not detected in specimens from these regions. DNA of R. rhipicephali was detected in D. occidentalis specimens obtained from Ensenada Municipality in Baja California and southwestern Oregon, but not from Washington. All ompA sequences of R. rhipichephali that were amplified from individual ticks in southwestern Oregon were represented by a single genotype. DNA of the Ixodes pacificus rickettsial endosymbiont was amplified from specimens collected in southwestern Oregon and Klickitat County, Washington; to the best of our knowledge, this Rickettsia species has never been identified in D. occidentalis. Collectively, these data are consistent with a relatively recent introduction of Pacific Coast ticks in the northernmost extension of its recognized range.
COVID-19 vaccination, infection, and symptoms among cancer survivors in the United States
Authors: Yang Nuo Nova , Zhao Jingxuan , Zheng Zhiyuan , Yabroff K Robin , Han Xuesong 
Published: February 2024

Abstract
The experiences of cancer survivors with the COVID-19 pandemic in the United States during 2021 and 2022, when vaccinations became widely available, are largely undocumented. Using nationally representative survey data in 2021 and 2022, we found that compared with adults without a cancer history, cancer survivors were more likely to have at least 2 COVID-19 vaccines (2021: 66.6% vs 62.3%, P = .003; 2022: 77.0% vs 72.4%, P &lt; .001) and as likely to have a COVID-19 infection history (2021: 14.1% vs 14.2%, P = .93; 2022: 39.9% vs 39.3%, P = .55) but, once infected, were more likely to develop moderate to severe symptoms (2021: 62.5% vs 54.2%, P = .02; 54.5% vs 61.3%; P = .13). Among cancer survivors, younger age, lower educational attainment, lack of health insurance, and more comorbidities were statistically significantly associated with lower vaccination rates (P &lt; .001). Among infected cancer survivors, being female and younger were associated with higher likelihood of developing moderate to severe symptoms (P &lt; .001). Our findings suggest tailored efforts to prevent and control COVID-19 infections for cancer survivors.
A customized technique for confluent aortic annular and selective sinus of Valsalva endocarditis
Authors: Barua Anupama , Nanjaiah Prakash , Warwick Richard , Jeeji Ravish , Balacumaraswami Lognathen 
Published: February 2024

Abstract
We describe a novel technical modification for reoperative aortic valve replacement in destructive recurrent prosthetic aortic valve endocarditis. We encountered complex anatomy in a previously operated aortic root wherein the aortic annulus and the right coronary sinus of Valsalva were destroyed. This precluded secure suture placement. We modified a composite mechanical Valsalva conduit to create a separate sinus of Valsalva left in continuity with the mechanical valve. This approach allowed us to exclude the infected right sinus of Valsalva and the corresponding aortic annulus.
Transmembrane formins as active cargoes of membrane trafficking
Authors: Cvrčková Fatima , Ghosh Rajdeep , Kočová Helena 
Published: February 2024

Abstract
Formins are a large, evolutionarily old family of cytoskeletal regulators whose roles include actin capping and nucleation, as well as modulation of microtubule dynamics. The plant class I formin clade is characterized by a unique domain organization, as most of its members are transmembrane proteins with possible cell wall-binding motifs exposed to the extracytoplasmic space—a structure that appears to be a synapomorphy of the plant kingdom. While such transmembrane formins are traditionally considered mainly as plasmalemma-localized proteins contributing to the organization of the cell cortex, we review, from a cell biology perspective, the growing evidence that they can also, at least temporarily, reside (and in some cases also function) in endomembranes including secretory and endocytotic pathway compartments, the endoplasmic reticulum, the nuclear envelope, and the tonoplast. Based on this evidence, we propose that class I formins may thus serve as ‘active cargoes’ of membrane trafficking—membrane-embedded proteins that modulate the fate of endo- or exocytotic compartments while being transported by them.
Spatial structure of resonance cavities in sunspots
Authors: Sych Robert , Zhu Xiaoshuai , Chen Yao , Yan Fabao 
Published: February 2024

ABSTRACT
We present a study of wave processes in sunspots from active regions NOAA 11131 on 2010 December 10 and NOAA 12565 on 2016 July 14 observed by SDO/AIA in the 1600, 304, and 171 Å temperature channels. To study the spatial structure of the resonance cavities previously found by Jess et al., we applied spectral data processing techniques such as pixelized wavelet filtering and mode decomposition. For the first time, we found stable regions as waveguides of the oscillations in the sunspot umbra, occupying specific frequency ranges without spatial overlap. The sizes of these regions depend on the frequency oscillations, and the maximum frequency coincides with the values of the harmonics of the main oscillation mode. Frequency drifts were observed in the band occupied by these regions, with different spectral slopes depending on the location of the sources in the sunspot umbra. We suggest that the observed distribution of wave sources in the umbra is a set of resonant cavities where successive amplification of oscillations at selected multiple harmonics is observed. The distribution of sources at low frequencies indicates the influence of the atmospheric cut-off due to the inclinations of the magnetic field lines.
Symmetry-breaking malachite green as a near-infrared light-activated fluorogenic photosensitizer for RNA proximity labeling
Authors: Li Lan , Han Jinghua , Lo Hei-Yong G , Tam Winnie Wai Ling , Jia Han , Tse Edmund Chun Ming , Taliaferro J Matthew , Li Ying 
Published: February 2024

Abstract
Cellular RNA is asymmetrically distributed in cells and the regulation of RNA localization is crucial for proper cellular functions. However, limited chemical tools are available to capture dynamic RNA localization in complex biological systems with high spatiotemporal resolution. Here, we developed a new method for RNA proximity labeling activated by near-infrared (NIR) light, which holds the potential for deep penetration. Our method, termed FAP-seq, utilizes a genetically encoded fluorogen activating protein (FAP) that selectively binds to a set of substrates known as malachite green (MG). FAP binding restricts the rotation of MG and rapidly activates its fluorescence in a wash-free manner. By introducing a monoiodo modification to MG, we created a photosensitizer (MG-HI) with the highest singlet oxygen generation ability among various MG derivatives, enabling both protein and RNA proximity labeling in live cells. New insights are provided in the transcriptome analysis with FAP-seq, while a deeper understanding of the symmetry-breaking structural arrangement of FAP–MG-HI was obtained through molecular dynamics simulations. Overall, our wash-free and NIR light-inducible RNA proximity labeling method (FAP-seq) offers a powerful and versatile approach for investigating complex mechanisms underlying RNA-related biological processes.
Mechanistic insights into the alternative ribosome recycling by HflXr
Authors: Seely Savannah M , Basu Ritwika S , Gagnon Matthieu G 
Published: February 2024

Abstract
During stress conditions such as heat shock and antibiotic exposure, ribosomes stall on messenger RNAs, leading to inhibition of protein synthesis. To remobilize ribosomes, bacteria use rescue factors such as HflXr, a homolog of the conserved housekeeping GTPase HflX that catalyzes the dissociation of translationally inactive ribosomes into individual subunits. Here we use time-resolved cryo-electron microscopy to elucidate the mechanism of ribosome recycling by Listeria monocytogenes HflXr. Within the 70S ribosome, HflXr displaces helix H69 of the 50S subunit and induces long-range movements of the platform domain of the 30S subunit, disrupting inter-subunit bridges B2b, B2c, B4, B7a and B7b. Our findings unveil a unique ribosome recycling strategy by HflXr which is distinct from that mediated by RRF and EF-G. The resemblance between HflXr and housekeeping HflX suggests that the alternative ribosome recycling mechanism reported here is universal in the prokaryotic kingdom.
Association of Race-Ethnicity Intersection With Disparities in Cigarette Smoking in U.S. Adults
Authors: Dai Hongying Daisy , Subica Andrew , Mattingly Delvon T , Harlow Alyssa , Leventhal Adam M 
Published: February 2024

Abstract

Introduction
Detailed estimates of disparities in cigarette smoking across single- and multi-race groups and their intersections with ethnicity are lacking. This study estimates the prevalence of self-reported current smoking among intersecting adult race-ethnicity groups in the United States.

Aims and Methods
The analysis uses 2018–2019 data from the Tobacco Use Supplement—Current Population Supplement (TUS-CPS; n = 137 471). Self-reported Hispanic origin and race were recoded into 19 mutually exclusive race-by-ethnicity intersecting groups. Weighted race-ethnicity group smoking prevalence were compared to the overall population prevalence and one another.

Results
Compared to the U.S. population current smoking prevalence (11.4% [95% CI = 11.2% to 11.6%]), smoking was particularly higher in non-Hispanic American Indian/Alaska Native (AI/AN) groups (20.7% [95% CI = 17.8% to 24.0%]) and non-Hispanic multiracial AI/AN/White (24.4% [95% CI = 20.3% to 29.1%]) and AI/AN/Black (22.4% [95% CI = 14.4% to 33.2%]) groups. Non-Hispanic single-race Asian (5.0% [95% CI = 4.4% to 5.6%]) and Hispanic single-race White (7.2% [95% CI = 6.7% to 7.7%]) smoking prevalence undercut the overall U.S. population prevalence. In pairwise comparisons, smoking prevalence was higher in various non-Hispanic single- and multi-race AI/AN groups than non-AI/AN groups and was lower in various Hispanic groups than non-Hispanic groups.

Conclusions
Smoking prevalence disparities are not monolithic across complex diversity of race and ethnicity in the United States. Accurate identification of priority populations in need of targeted tobacco control efforts may benefit by acknowledging multinomial heterogeneity across intersecting racial and ethnic identities.

Implications
Understanding racial and ethnic disparities in cigarette smoking can inform national strategies for reducing health inequities. This study examines cigarette smoking disparities among 19 adult intersecting race-ethnicity groups in the United States using the 2018–2019 TUS-CPS data. Results show higher smoking rates in some non-Hispanic American Indian/Alaska Native and Black groups, while lower rates are seen in Asian and Hispanic individuals. These findings emphasize the need for disaggregated data to tackle smoking disparities and guide targeted prevention efforts.
Chemical Composition of Electronic Vaping Products From School Grounds in California
Authors: Wang Ping , Williams Rebecca J , Chen Wenhao , Wang Flavia , Shamout Mays , Tanz Lauren J , Herzig Carolyn T A , Oakley Lisa P , Peak Corey M , Heinzerling Amy , Al-Shawaf Maeh , Melstrom Paul , Marynak Kristy , Tynan Michael A , Agaku Israel T , Kumagai Kazukiyo 
Published: February 2024

Abstract

Introduction
The use of electronic vaping products (EVPs) containing nicotine, marijuana, and/or other substances remains prominent among youth; with EVPs containing nicotine being the most commonly used tobacco product among youth since 2014. However, a detailed understanding of the chemical composition of these products is limited.

Aims and Methods
From February 25th to March 15th, 2019, a total of 576 EVPs, including 233 e-cigarette devices (with 43 disposable vape pens) and 343 e-liquid cartridges/pods/bottled e-liquids, were found or confiscated from a convenience sample of 16 public high schools in California. Liquids inside 251 vape pens and cartridges/pods/bottled e-liquids were analyzed using a gas chromatography/mass spectrometry (GC/MS). For comparison, new JUUL pods, the most commonly used e-cigarette among youth during 2018–2019, with different flavorings and nicotine content were purchased and analyzed.

Results
For e-cigarette cartridges/pods/bottled e-liquids, nicotine was detected in 204 of 208 (98.1%) samples. Propylene glycol and vegetable glycerin were dominant solvents in nicotine-containing EVPs. Among 43 disposable vape pen devices, cannabinoids such as tetrahydrocannabinol (THC) or cannabidiol (CBD) were identified in 39 of 43 (90.1%) samples, of which three contained both nicotine and THC. Differences in chemical compositions were observed between confiscated or collected JUULs and purchased JUULs. Measured nicotine was inconsistent with labels on some confiscated or collected bottled e-liquids.

Conclusions
EVPs from 16 participating schools were found to widely contain substances with known adverse health effects among youth, including nicotine and cannabinoids. There was inconsistency between labeled and measured nicotine on the products from schools.

Implications
This study measured the main chemical compositions of EVPs found at 16 California public high schools. Continued efforts are warranted, including at the school-level, to educate, prevent, and reduce youth use of EVPs.
High inter-population connectivity and occasional gene flow between subspecies improves recovery potential for the endangered Least Bell’s Vireo
Authors: Vandergast Amy G , Kus Barbara E , Wood Dustin A , Mitelberg Anna , Smith Julia G , Milano Elizabeth R 
Published: February 2024

Abstract
Increasingly, genomic data are being used to supplement field-based ecological studies to help evaluate recovery status and trends in endangered species. We collected genomic data to address 2 related questions regarding the Least Bell’s Vireo (Vireo bellii), an endangered migratory songbird restricted to southern California riparian habitat for breeding. First, we sought to delineate the range limits and potential overlap between Least Bell’s Vireo and its sister subspecies, the Arizona Bell’s Vireo, by analyzing samples from the deserts of eastern California, southwestern Nevada, Utah, and Arizona. Second, we evaluated genetic structure among Least Bell’s Vireo populations in coastal California and estimated effective population size. Clustering analyses based on 10,571 single-nucleotide polymorphisms (SNPs) from 317 samples supported 2 major groups that aligned closely to the previously defined subspecies ranges. The first cluster included birds in the Central Valley, all coastal drainages, and westernmost deserts of California, with no further sub-structuring among coastal drainages. Almost all birds from the Amargosa River in eastern California and eastward assigned to the second cluster; however, low levels of gene flow were detected across the subspecies groups, with greater rates of gene flow from Arizona Bell’s Vireo to Least Bell’s Vireo than the reverse. Admixed individuals occurred in the California deserts; and although smaller than coastal populations, desert populations may be important for maintaining and replenishing genetic diversity and facilitating the movement of potentially adaptive genes between subspecies. Within Least Bell’s Vireo, local populations in coastal drainages comprised a single genetic population, with some evidence of close relatives distributed across drainages, suggesting that these could function as a well-connected metapopulation. These results are consistent with previous Least Bell’s Vireo banding studies that reported high rates of dispersal among drainages. Effective population size for both subspecies was high, suggesting that adaptive potential has been maintained despite previous declines.
The Logic and Mathematics of Charles Sanders Peirce
Authors: Kauffman Louis H. 
Published: February 2024

AbstractThis chapter explores the logic and mathematics of Charles Sanders Peirce. The chapter begins with Peirce’s sign of illation, a sign for implication that is a structural combination of the operations of or and negation. This sign is a direct notational relative of the mark of George Spencer-Brown. The work of Gottlob Frege and Spencer-Brown is compared with that of Peirce, and it is shown how to translate Peirce’s theory of existential graphs with Frege’s tree-like logical notations and how these can be superimposed on the calculus of Spencer-Brown to give a uniform method for logical quantifiers in each of these domains. Spencer-Brown’s formalisms for self-reference and re-entry are discussed in relation to Peirce’s concept of a sign for itself, and these ideas of Peirce are discussed in relation to the mathematics of infinity and infinitesimals. Peirce’s notions about infinitesimals are compared with intuitionistic logic and with the Heyting algebra of open sets in a topological space. The chapter shows the remarkable connections of Peirce’s ideas with mathematical foundations.
Pragmatisms?
Authors: Kitcher Philip 
Published: February 2024

Abstract
Though Charles Peirce is oft regarded as the founder of pragmatism, it is not entirely clear whether there really is a sufficiently unified movement that can be called pragmatism. Though William James cites Peirce’s pragmatic maxim when introducing his own pragmatism, and John Dewey refers to the maxim as well, this chapter shows that the three “pragmatisms” diverge at the root, going in very different directions. Alternative attempts at seeking unity—their views on truth and the doubt–belief model of inquiry—also fail to bring the three thinkers close enough together. Consequently, though they all take a revisionist approach to philosophy and embrace pragmatism on that ground, they differ considerably in how to bring philosophy back to its proper task.