bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zooplanktivory in garden eels: benefits and shortcomings of being “anchored” compared with other coral-reef fish
Authors: Khrizman Alexandra , Kolesnikov Irena , Churilov Dmitri , Genin Amatzia 
Published: March 2024

Garden eels are elongated zooplanktivorous fish that live in colonies on sandy bottoms, often adjacent to coral reefs. Each eel digs its own burrow, from which it partially emerges to forage on drifting zooplankton while being “anchored” with its tail inside the burrow. Feeding rates and foraging movements were examined in the garden eel Gorgasia sillneri and compared with corresponding measurements carried out as part of this study and by (Genin et al.)1 with 3 species of “free”, site-attached coral-reef fish. Feeding rates by the garden eels were substantially lower than those of the free fish. In the eels, those rates monotonically increased with increasing current speed up to ~20 cm/s, whereas in the free fish maximum rates were observed under moderate flows. A nearly linear increase in feeding rate as function of prey density was observed in both the garden eels and the free fish. However, the slope of that increase in the eels was over an order of magnitude more gradual than that reported for the free fish. The different functional responses of the two fish groups appear to be related to their morphology and maneuverability capabilities. Being elongated, anchored in a burrow and able to modulate body posture according to the flow speed allow the eels high feeding rates under strong currents. The tradeoff, compared with free fish, include limited maneuverability, slower swimming, and smaller foraging volume, rendering the eels’ functional response less efficient to increasing prey density. This cost appears to be compensated by the eels’ ability to occupy sandy, shelter-less bottoms, which in some locations are immensely more abundant than coral-covered rocks, where most planktivorous free fish live.
Acute and chronic central nervous system oxidative stress/toxicity during hyperbaric oxygen treatment of subacute and chronic neurological conditions
Authors: Harch Paul G. , Rhodes Stacey 
Published: March 2024

IntroductionOxygen toxicity has been defined as acute central nervous system (CNS), acute pulmonary, and chronic pulmonary oxygen toxicity. This study identifies acute and chronic CNS oxygen toxicity under 2.0 atmospheres absolute (ATA) pressure of oxygen. Methods: The authors’ medical records from September 29, 1989 to January 20, 2023 and correspondence to the authors (9/1994 to 1/20.2023) from patients with signs and/or symptoms historically identified as acute CNS oxygen toxicity and those with neurological deterioration receiving hyperbaric oxygen for neurological conditions were reviewed. Acute cases were those occurring with ≤5 HBOTs and chronic cases &gt;5 HBOTs. Chronic cases were separated into those at 1.5 ATA, &gt; 1.5 ATA, or &lt; 1.5 ATA oxygen. Cumulative dose of oxygen in atmosphere-hours (AHs) was calculated at symptom onset.ResultsSeven acute cases, average 4.0 ± 2.7 AHs, and 52 chronic cases were identified: 31 at 1.5 ATA (average 116 ± 106 AHs), 12 at &gt;1.5 ATA (103 ± 74 AHs), and 9 at &lt;1.5 ATA (114 ± 116 AHs). Second episodes occurred at 81 ± 55, 67 ± 49, and 22 ± 17 AHs, and three or more episodes at 25 ± 18, 83 ± 7.5, and 5.4 ± 6.0 AHs, respectively. Most cases were reversible. There was no difference between adults and children (p = 0.72). Acute intervention in cases (&lt;3 months) was more sensitive than delayed intervention (21.1 ± 8.8 vs. 123 ± 102 AHs, p = 0.035). Outside sources reported one acute and two chronic exposure deaths and one patient institutionalized due to chronic oxygen toxicity. A withdrawal syndrome was also identified.ConclusionHyperbaric oxygen therapy-generated acute and chronic cases of CNS oxygen toxicity in chronic neurological conditions were identified at &lt;2.0 ATA. Chronic CNS oxygen toxicity is idiosyncratic, unpredictable, and occurred at an average threshold of 103–116 AHs with wide variability. There was no difference between adults and children, but subacute cases were more sensitive than chronic intervention cases. When identified early it was reversible and an important aid in proper dosing of HBOT. If ignored permanent morbidity and mortality resulted with continued HBOT.
Analyses of hypoxia-related risk factors and clinical relevance in breast cancer
Authors: Li Yan , Yu Haiyang , Han Xinghua , Pan Yueyin 
Published: March 2024

IntroductionHypoxia plays an important role in the heterogeneity, relapse, metastasis, and drug resistance of breast cancer. In this study, we explored the hypoxia-related biological signatures in different subtypes of breast cancer and identified the key prognostic factors by bioinformatics methods.MethodsBased on The Cancer Genome Atlas (TCGA) Breast Cancer datasets, we divided the samples into immune-activated/suppressed populations by single-sample gene set enrichment analysis (ssGSEA) and then used hierarchical clustering to further identify hypoxic/non-hypoxic populations from the immune-suppressed samples. A hypoxia related risk model of breast cancer was constructed.ResultsNuclear factor interleukin-3 regulated (NFIL3), serpin family E member 1 (SERPINE1), FOS, biglycan (BGN), epidermal growth factor receptor (EGFR), and sushi-repeat-containing protein, X-linked (SRPX) were identified as key hypoxia-related genes. Margin status, American Joint Committee on Cancer (AJCC) stage, hypoxia status, estrogen receptor/progesterone receptor (ER/PR) status, NFIL3, SERPINE1, EGFR, and risk score were identified as independent prognostic indicators for breast cancer patients. The 3- and 5-year survival curves of the model and immunohistochemical staining on the breast cancer microarray verified the statistical significance and feasibility of our model. Among the different molecular types of breast cancer, ER/PR+ and HER2+ patients might have higher hypoxia-related risk scores. ER/PR-negative samples demonstrated more activated immune-related pathways and better response to most anticancer agents.DiscussionOur study revealed a novel risk model and potential feasible prognostic factors for breast cancer and might provide new perspectives for individual breast cancer treatment.
Clinical and laboratory parameters associated with febrile seizure recurrence within the first 24 h: a ten-year cohort study
Authors: Castellazzi Massimo Luca , La Vecchia Adriano , Scali Martina , Agostoni Carlo , Di Pietro Giada , Milani Gregorio Paolo 
Published: March 2024

IntroductionWe assessed clinical and laboratory parameters associated with early recurrence of febrile seizure in patients presenting at the Emergency Department with a first episode.MethodsCase series of patients admitted to the emergency department with the first episode of febrile seizure for ten consecutive years. Exclusion criteria were focal features and prolonged duration (&gt;15 min).ResultsWe included 693 patients, 284 (41%) female. Median age of 20 (IQR 15–27) months. Fifty-two (8%) patients had a recurrence within 24 h. At univariate analysis, patients with recurrent seizures had higher use of antipyretics (88% vs. 74%, P = 0.03, OR 2.6, 95% CI: 1.1–7.7), higher median maximal body temperature (39.3 °C, IQR 38.9–39.9, vs. 38.9, IQR 38.4–39.3, P &lt; 0.001, OR 2.3, 95% CI: 1.5–2.6) and presented with a lower proportion of respiratory tract infections (54% vs. 70%, P = 0.02) compared to patients without recurrence. A maximal body temperature equal to or higher than 39 °C was associated with a higher recurrence (11% vs. 4%, P &lt; 0.001, OR 2.9, 95% CI: 1.6–5.6). Hyponatremia was not associated with a risk of recurrence. The multivariate analysis confirmed a direct association with body temperature (OR 2.3, 95% CI: 1.5–3.7, P &lt; 0.001), and an inverse association with respiratory tract infections (OR 0.4, 95% CI: 0.2–0.9, P = 0.01), while antipyretic use was not correlated (OR 1.9, 95% CI: 0.8–5.2, P = 0.2).ConclusionsHigh body temperature and respiratory tract infections were (directly and inversely) associated with recurrences. Consideration of these conditions might help for anticipating the probability of recurrence.
Exploring inter-ethnic and inter-patient variability and optimal dosing of osimertinib: a physiologically based pharmacokinetic modeling approach
Authors: Liang Feng , Zhang Yimei , Xue Qian , Yao Na 
Published: March 2024

Purpose: This study aimed to develop and validate a physiologically based pharmacokinetic (PBPK) model for osimertinib (OSI) to predict plasma trough concentration (Ctrough) and pulmonary EGFRm+ (T790M and L858R mutants) inhibition in Caucasian, Japanese, and Chinese populations. The PBPK model was also utilized to investigate inter-ethnic and inter-patient differences in OSI pharmacokinetics (PK) and determine optimal dosing regimens.Methods: Population PBPK models of OSI for healthy and disease populations were developed using physicochemical and biochemical properties of OSI and physiological parameters of different groups. And then the PBPK models were validated using the multiple clinical PK and drug-drug interaction (DDI) study data.Results: The model demonstrated good consistency with the observed data, with most of prediction-to-observation ratios of 0.8–1.25 for AUC, Cmax, and Ctrough. The PBPK model revealed that plasma exposure of OSI was approximately 2-fold higher in patients compared to healthy individuals, and higher exposure observed in Caucasians compared to other ethnic groups. This was primarily attributed to a lower CL/F of OSI in patients and Caucasian. The PBPK model displayed that key factors influencing PK and EGFRm+ inhibition differences included genetic polymorphism of CYP3A4, CYP1A2 expression, plasma free concentration (fup), albumin level, and auto-inhibition/induction on CYP3A4. Inter-patient PK variability was most influenced by CYP3A4 variants, fup, and albumin level. The PBPK simulations indicated that the optimal dosing regimen for patients across the three populations of European, Japanese, and Chinese ancestry was OSI 80 mg once daily (OD) to achieve the desired range of plasma Ctrough (328–677 nmol/L), as well as 80 mg and 160 mg OD for desirable pulmonary EGFRm+ inhibition (&gt;80%).Conclusion: In conclusion, this study’s PBPK simulations highlighted potential ethnic and inter-patient variability in OSI PK and EGFRm+ inhibition between Caucasian, Japanese, and Chinese populations, while also providing insights into optimal dosing regimens of OSI.
Establishment and application of Agrobacterium-delivered CRISPR/Cas9 system for wild tobacco (Nicotiana alata) genome editing
Authors: Yuan Cheng , Zeng Jianmin , Liu Yong , Yu Haiqin , Tong Zhijun , Zhang Jianduo , Gao Qian , Wang Zhong , Sui Xueyi , Xiao Bingguang , Huang Changjun 
Published: March 2024

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system has been widely applied in cultivated crops, but limited in their wild relatives. Nicotiana alata is a typical wild species of genus Nicotiana that is globally distributed as a horticultural plant and well-studied as a self-incompatibility model. It also has valuable genes for disease resistance and ornamental traits. However, it lacks an efficient genetic transformation and genome editing system, which hampers its gene function and breeding research. In this study, we developed an optimized hypocotyl-mediated transformation method for CRISPR-Cas9 delivery. The genetic transformation efficiency was significantly improved from approximately 1% to over 80%. We also applied the CRISPR-Cas9 system to target the phytoene desaturase (NalaPDS) gene in N. alata and obtained edited plants with PDS mutations with over 50% editing efficiency. To generate self-compatible N. alata lines, a polycistronic tRNA-gRNA (PTG) strategy was used to target exonic regions of allelic S-RNase genes and generate targeted knockouts simultaneously. We demonstrated that our system is feasible, stable, and high-efficiency for N. alata genome editing. Our study provides a powerful tool for basic research and genetic improvement of N. alata and an example for other wild tobacco species.
The history of families at-risk for hereditary breast and ovarian cancer: what are the impacts of genetic counseling and testing?
Authors: Campacci Natalia , Grasel Rebeca Silveira , Galvão Henrique de Campos Reis , Garcia Lucas França , Ribeiro Paula Carvalho , Pereira Kercy Fram de Jesus de Sena , Goldim José Roberto , Ashton-Prolla Patricia , Palmero Edenir Inêz 
Published: March 2024

IntroductionCancer Genetic Counseling (CGC) and genetic testing (GT) assume a paramount role for hereditary cancer predisposition syndrome families. We assessed the effects of CGC and GT on women affected by cancer who are at risk for hereditary breast and ovarian cancer predisposition syndrome (HBOC).MethodsThis study encompasses four time points: before the CGC session, after the CGC session when blood is drawn for GT, after disclosure of GT results, and six months following disclosure of GT results. The impacts of CGC and GT were assessed using psychosocial questionnaires. Additionally, a pedigree, genogram, and ecomap were constructed through a semistructured interview.ResultsA total of sixty women were included in the study. Most participants considered their perception of cancer risk to be equivalent to that of the general population, even among those with pathogenic variants. An increased perception of breast and ovarian cancer risks was associated with a heightened inclination toward religious engagement as a coping mechanism. Patients carrying variants of uncertain significance expressed greater concerns about developing another cancer compared to those who had BRCA1 and BRCA2 wild type or pathogenic variants. Qualitative analysis of the genograms and ecomaps demonstrated that the CGC/GT processes facilitate communication within families. The genogram analyses revealed the impact of CGC and GT processes on families at risk for hereditary cancer. Changes in some family relationships were observed, and an improvement in communication was noted following the GT process.DiscussionThese findings can assist healthcare professionals considering a personalized approaches in clinical practice.
“It doesn’t matter if we’re the most amazing professionals in the world…” A qualitative study of professionals’ perspectives on parent-child interaction assessment with deaf infants
Authors: Curtin Martina , Wakefield Tina , Herman Rosalind , Morgan Gary , Cruice Madeline 
Published: March 2024

IntroductionParent child interaction (PCI) is positively associated with deaf children’s language development. However, there are no known, deaf-specific tools to observe how a parent interacts with their deaf child aged 0–3 years. Without a framework for professionals to use with families, it is unknown how professionals assess PCI, what they assess, why they assess, and how the assessment results relate to case management.MethodsEighteen hearing and deaf professionals, who work with deaf and hard of hearing infants aged 0–3 years and their families, attended online focus groups. The aim of the study was to gain insight into the professional assessment of PCI. Data were analyzed using thematic analysis.FindingsSix themes were generated from the dataset. Professionals discussed how central parents were in the support offered to families in the home, the importance of knowing and understanding the individual family, and accounting for and supporting parental wellbeing. Descriptions on how to administer a best practice PCI assessment included which parent behaviors to assess and how to make adaptations for different populations. Professionals shared how the assessment and review process could be used to inform and upskill parents through video reflection and goal setting.DiscussionThis study provides insight into the mechanisms and motivations for professionals assessing the interactive behaviors of parents who have deaf children aged 0–3. Professionals acknowledged that family life is multi-faceted, and that support is most meaningful to families when professionals worked with these differences and incorporated them into assessment, goal setting, and intervention plans.
Taking appreciation to heart: appreciation at work and cardiovascular risk in male employees
Authors: Auer Alisa , Semmer Norbert K. , von Känel Roland , Thomas Livia , Zuccarella-Hackl Claudia , Wiest Roland , Wirtz Petra H. 
Published: March 2024

IntroductionWhile perceived appreciation at work has been associated with self-reported health and wellbeing, studies considering biological health markers are lacking. In this study, we investigated whether appreciation at work would relate to coronary heart disease (CHD) risk as well as the specificity of this proposed association.MethodsOur study comprised a total of 103 male participants, including apparently healthy, medication-free, non-smoking men in the normotensive to hypertensive range (n = 70) as well as medicated hypertensive and CHD patients (n = 33). CHD risk was assessed by blood pressure [mean arterial pressure (MAP)], the diabetes marker glycated hemoglobin A1c (HbA1c), blood lipids [total cholesterol (TC)/high-density lipoprotein-cholesterol (HDL-C) ratio], coagulation activity (D-dimer and fibrinogen), and inflammation [interleukin (IL)-6, tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP)]. Perceived appreciation at work, as well as potentially confounding psychological factors (social support, self-esteem, and work strain due to a lack of appreciation), were measured by self-report questionnaires.ResultsWe found higher appreciation at work to relate to lower overall composite CHD risk (p’s ≤ 0.011) and, in particular, to lower MAP (p’s ≤ 0.007) and lower blood lipids (p’s ≤ 0.031) in medication-free participants as well as all participants. This overall association was independent of confounding factors, including related psychological factors (p’s ≤ 0.049).DiscussionOur findings indicate that appreciation at work might be an independent health-promoting resource in terms of CHD risk. Implications include that encouraging appreciation at work may help reduce the development and progression of CHD.
The ecosystem of human capital in care homes
Authors: Kejžar Anamarija , Turunen Katri Maria 
Published: March 2024

IntroductionThis qualitative study addresses the essential yet often overlooked experiences of knowledge transfer within care homes (CH). Conducted in a Slovenian CH in 2020 and 2023, participants, including CH management, staff, and residents with their relatives, shared perceptions of knowledge transfer at various levels. The study aims to explore barriers and facilitators for knowledge transfer crucial for creating new knowledge, services, and enhancing care quality for older individuals.MethodsStructured focus group interviews were conducted, and data were collected within the CH. The participants’ insights into knowledge transfer were probed, covering various dimensions such as between individuals, groups, organizations, and the community. Transcriptions of recorded interviews were analyzed using content analysis.ResultsKnowledge transfer within the CH was facilitated through continuous training, diverse communication channels, and mentoring. Collaboration with relatives improved understanding of resident preferences, habits, and overall enhanced the quality of care. This collaborative effort allowed mutual learning and knowledge transfer from the CH to the broader community. Despite potential benefits, there is an underutilization of information and communication technology, e-care, and untapped potential for partnerships, partly due to the scarcity of care. Barriers were identified in the form of stereotypical attitudes towards aging and care, further reinforced by negative news coverage on older people’s care.ConclusionThe multidimensional nature of knowledge in CH centers on resident well-being, emphasizing three key aspects of knowledge transfer: between staff and residents, staff and residents’ families, and between the CH and the community. In the context of age management, creating opportunities for knowledge transfer is crucial, emphasizing a transition from traditional institutional care to an approach prioritizing knowledge about quality care. and involving experts from experiences in care process.
Through each other's eyes: initial results and protocol for the co-design of an observational measure of adolescent-parent interaction using first-person perspective
Authors: Wright Nicky , Pearson Rebecca M. , Crook Danielle , Bond Alice , Jewell Tom 
Published: March 2024

BackgroundCurrent observational methods to understand adolescent-parent interaction are limited in terms of ecological and content validity. We outline initial results and a protocol for future work from a programme of work to: (1) establish a new method for data capture of adolescent-parent interaction at home using wearable cameras and; (2) develop a new relevant and comprehensive observational micro-coding scheme. In Part 1, we report our completed preliminary work, comprised of an initial scoping review, and public engagement work. In Part 2, we present a protocol for the development of the new measure.MethodsPart 1—We searched Pubmed for existing observational measures of adolescent-parent interaction for the scoping review. We also undertook public engagement work utilising a mobile research van, taken to multiple locations around Bristol, UK to engage with a variety of populations through interactive methods. Part 2—Our protocol describes plans for: (1) A systematic review of the psychometric properties of observational measures of adolescent-parent interaction; (2) Focussed public engagement workshops; (3) Harmonisation of information from existing coding schemes and literature with information from public engagement with adolescents and parents; (4) A pilot study to assess the acceptability and feasibility of the method; (5) Development of a coding scheme in consultation with expert and lay panels, and through real-life application to recorded videos from a pilot sample.ResultsScoping review: we identified 21 adolescent-parent observational schemes, of which eight used micro-coding and 13 used globalcoding schemes. The majority of micro-coding schemes were not developed specifically for adolescents. Most studies used conflict or problem-solving tasks, which may not adequately capture positive adolescent-parent interactions. The mobile van event received views from 234 young people and/or parents. Families were positive about taking part in research using headcams. “Trust” and “understanding” were most frequently reported as important adolescent-parent relationship constructs.ConclusionsThis work represents the first attempt to truly co-design a method to assess parenting in adolescence. We hope to develop an observational measure using novel technological methods that can be used across a range of research and therapeutic settings.