bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nongenomic effect of estrogen on the MAPK signaling pathway and calcium influx in endometrial carcinoma cells
Authors: Zhang Lili , Li Xiaoping , Zhao Lijun , Zhang Lifang , Zhang Guo , Wang Jianliu , Wei Lihui 
Published: January 2009

Abstract17β‐Estradiol (E2) is well known to interact with intracellular receptors that act as nuclear transcription factors. However, abundant evidence now indicates that E2 can also rapidly induce several nongenomic effects through signaling pathways related to cell growth, preservation, and differentiation. We studied the nongenomic effects of E2 in two human endometrial carcinoma cell lines, Ishikawa (estrogen receptor (ER) positive) and Hec‐1A (ER negative or low) by cultivating them with either E2 or its membrane‐impermeable conjugate, E2–BSA. We found that phosphorylation of Erk1/2 could be induced by either E2 or E2–BSA in Ishikawa cells. In Hec‐1A cells, only E2 was able to induce Erk1/2 phosphorylation. Although the existence of a nongenomic component to the response was indicated by the finding that it could not be completely inhibited by the ER antagonist ICI182780,and it can also be inhibited by calcium inhibitor Nifedipine partly. Phosphorylation of Akt could not be induced, either by E2 or E2–BSA, in either cell line. Both E2 and E2–BSA elicited calcium influx in Ishikawa cells. In contrast to these nongenomic effects, only E2 was able to stimulate expression of the anti‐apoptotic‐protein Bcl‐2. Taken together, these data indicate that nongenomic effects such as Erk1/2 phosphorylation and calcium influx can be initiated from the membrane in Ishikawa cell, and calcium can activate Erk1/2 phosphorylation. Except for ER, there must be other binding location of estrogen in endometrial cancer cells, and the nongenomic effects of estrogen initiated from plasma membrane by E2–BSA cannot lead to transcriptional effect of Bcl‐2 expression. J. Cell. Biochem. 106: 553–562, 2009. © 2009 Wiley‐Liss, Inc.
Adenosine triggers the nuclear translocation of protein kinase C epsilon in H9c2 cardiomyoblasts with the loss of phosphorylation at Ser729
Authors: Xu Tian‐Rui , He Guiyuan , Rumsby Martin G. 
Published: January 2009

AbstractAdenosine is a major mediator of ischaemic preconditioning (IPC) and cardioprotection. The translocation and activation of protein kinase C epsilon, triggered by adenosine, are essential for these processes. We report here that H9c2 cardiomyoblasts express five PKC isoforms (α, βI, δ, ε and ζ). PKCε is predominantly associated with F‐actin fibres in unstimulated H9c2 cells but translocates to the nucleus on stimulation with adenosine. Cytosolic PKCε associated with F‐actin fibres is phosphorylated at Ser729 but nuclear PKCε lacks phosphorylation at this site. Adenosine triggers the nuclear translocation after 5 min stimulation. PKCε Ser729Ala and Ser729Glu mutants showed no translocation on adenosine stimulation suggesting both phosphorylation and serine at 729 are critical for this translocation. Among five PKC isoforms (α, βI, δ, ε and ζ) detected, PKCε is the only isoform translocating to the nucleus upon adenosine stimulation. Disruption of microtubules (MTs), but not F‐actin‐rich fibres, blocked translocation of both endogenous PKCε and overexpressed GFP‐PKCε to the nucleus. Ten proteins interacted with cytosolic PKCε; five of which are components of myofibrils. Matrin 3 and vimentin interacted with nuclear PKCε. These findings suggest that adenosine stimulates PKCε translocation to the nucleus in H9c2 cells in a mechanism involving dephosphorylation at Ser729 and MT, which should advance our understanding of the signalling pathways stimulated by adenosine in IPC and cardioprotection. J. Cell. Biochem. 106: 633–642, 2009. © 2009 Wiley‐Liss, Inc.
The nucleosomal binding protein NSBP1 is highly expressed in the placenta and modulates the expression of differentiation markers in placental Rcho‐1 cells
Authors: Shirakawa Hitoshi , Rochman Mark , Furusawa Takashi , Kuehn Michael R. , Horigome Satoru , Haketa Keiichi , Sugita Yumi , Inada Tomoyuki , Komai Michio , Bustin Michael 
Published: January 2009

AbstractWe report that NSBP1, a nucleosome binding protein that affects the structure of chromatin, is highly expressed in mouse placenta. In Rcho‐1 cells, which recapitulate the differentiation of trophoblast giant cells of living placenta, NSBP1 expression is linked to differentiation. Disregulation of NSBP1 protein levels, by either siRNA treatment or by overexpression, alters the expression of several members of the prolactin gene family without affecting the levels of several transcription factors involved in placental differentiation. Our studies identify NSBP1 as a nucleosome binding protein that modulates the expression of prolactin gene family members most likely by inducing changes in chromatin structure. J. Cell. Biochem. 106: 651–658, 2009. © 2009 Wiley‐Liss, Inc.
The important role of the apoptotic effects of zinc in the development of cancers
Authors: Franklin Renty B. , Costello Leslie C. 
Published: January 2009

AbstractZinc is a trace element that is essential for the normal function of cells. It is a cofactor for the structure and function of a wide range of cellular proteins including enzymes, transcription factors, and structural proteins. Recent studies have shown that zinc plays a role in the development of various cancers. Unfortunately no established common relationships of zinc with cancer development and progression have been identified. Zinc is known to have systemic effects such as regulation of the immune system as well as direct cellular effects resulting in regulation of gene expression, bioenergetics, metabolic pathways, signal transduction and cell invasion. Zinc is also reported to regulate cell proliferation and growth. In this review presentation we focus on the effects of zinc that are involved in the regulation of apoptosis in malignant cells. We selected the apoptotic effects of zinc because zinc is reported to both induce apoptosis in some cancers and to protect other cancer cells against apoptosis induced by other factors. The effects of zinc in the regulation of apoptosis appear to be cell type specific. More importantly the reported effects of zinc on cancer cells must be viewed from the perspective of the physiological regulation of zinc homeostasis. Thus one must be mindful of the experimental conditions under which zinc effects are investigated relative to the physiological and pathological conditions of cellular zinc distribution and concentrations that can exist in situ. J. Cell. Biochem. 106: 750–757, 2009. © 2009 Wiley‐Liss, Inc.
Cellular and functional analysis of four mutations located in the mitochondrial ATPase6 gene
Authors: Vazquez‐Memije Martha Elisa , Rizza Teresa , Meschini Maria Chiara , Nesti Claudia , Santorelli Filippo Maria , Carrozzo Rosalba 
Published: January 2009

AbstractThe smallest rotary motor of living cells, F0F1‐ATP synthase, couples proton flow—generated by the OXPHOS system—from the intermembrane space back to the matrix with the conversion of ADP to ATP. While all mutations affecting the multisubunit complexes of the OXPHOS system probably impact on the cell's output of ATP, only mutations in complex V can be considered to affect this output directly. So far, most of the F0F1‐ATP synthase variations have been detected in the mitochondrial ATPase6 gene. In this study, the four most frequent mutations in the ATPase6 gene, namely L156R, L217R, L156P, and L217P, are studied for the first time together, both in primary cells and in cybrid clones. Arginine (“R”) mutations were associated with a much more severe phenotype than Proline (“P”) mutations, in terms of both biochemical activity and growth capacity. Also, a threshold effect in both “R” mutations appeared at 50% mutation load. Different mechanisms seemed to emerge for the two “R” mutations: the F1 seemed loosely bound to the membrane in the L156R mutant, whereas the L217R mutant induced low activity of complex V, possibly the result of a reduced rate of proton flow through the A6 channel. J. Cell. Biochem. 106: 878–886, 2009. © 2009 Wiley‐Liss, Inc.
Identification and expansion of human osteosarcoma‐cancer‐stem cells by long‐term 3‐aminobenzamide treatment
Authors: Di Fiore Riccardo , Santulli Andrea , Drago Ferrante Rosa , Giuliano Michela , De Blasio Anna , Messina Concetta , Pirozzi Giuseppe , Tirino Virginia , Tesoriere Giovanni , Vento Renza 
Published: January 2009

AbstractA novel cancer stem‐like cell line (3AB‐OS), expressing a number of pluripotent stem cell markers, was irreversibly selected from human osteosarcoma MG‐63 cells by long‐term treatment (100 days) with 3‐aminobenzamide (3AB). 3AB‐OS cells are a heterogeneous and stable cell population composed by three types of fibroblastoid cells, spindle‐shaped, polygonal‐shaped, and rounded‐shaped. With respect to MG‐63 cells, 3AB‐OS cells are extremely smaller, possess a much greater capacity to form spheres, a stronger self‐renewal ability and much higher levels of cell cycle markers which account for G1‐S/G2‐M phases progression. Differently from MG‐63 cells, 3AB‐OS cells can be reseeded unlimitedly without losing their proliferative potential. They show an ATP‐binding cassette transporter ABCG2‐dependent phenotype with high drug efflux capacity, and a strong positivity for CD133, marker for pluripotent stem cells, which are almost unmeasurable in MG‐63 cells. 3AB‐OS cells are much less committed to osteogenic and adipogenic differentiation than MG‐63 cells and highly express genes required for maintaining stem cell state (Oct3/4, hTERT, nucleostemin, Nanog) and for inhibiting apoptosis (HIF‐1α, FLIP‐L, Bcl‐2, XIAP, IAPs, and survivin). 3AB‐OS may be a novel tumor cell line useful for investigating the mechanisms by which stem cells enrichment may be induced in a tumor cell line. The identification of a subpopulation of cancer stem cells that drives tumorigenesis and chemoresistance in osteosarcoma may lead to prognosis and optimal therapy determination. Expression patterns of stem cell markers, especially CD133 and ABCG2, may indicate the undifferentiated state of osteosarcoma tumors, and may correlate with unfavorable prognosis in the clinical setting. J. Cell. Physiol. 219: 301–313, 2009. © 2009 Wiley‐Liss, Inc.
Ion/Molecule reactions in SiH4/H2S and GeH4/H2S mixtures
Authors: Antoniotti Paola , Operti Lorenza , Rabezzana Roberto , Turco Francesca , Vaglio Gian Angelo , Grandinetti Felice 
Published: January 2009

AbstractThe gas phase ion chemistry of silane/hydrogen sulfide and germane/hydrogen sulfide mixtures was studied by ion trap mass spectrometry (ITMS), in both positive and negative ionization mode. In positive ionization, formation of X/S (X = Si, Ge) mixed ions mainly takes place via reactions of silane or germane ions with H2S, through condensation followed by dehydrogenation. This is particularly evident in the system with silane. On the other side, reactions of HnS2+ ions with XH4 (X = Si, Ge) invariably lead to formation of a single XS bond. In negative ionization, a more limited number of mixed ion species is detected, but their overall abundance reaches appreciable values, especially in the SiH4/H2S system. Present results clearly indicate that ion processes play an important role in formation and growth of clusters eventually leading to deposition of amorphous solids in chemical vapor deposition (CVD) processes. Copyright © 2009 John Wiley & Sons, Ltd.
Desorption electrospray ionization mass spectrometry of DNA nucleobases: implications for a liquid film model
Authors: Qiu Bo , Luo Hai 
Published: January 2009

AbstractDesorption electrospray ionization (DESI) mass spectrometry has been implemented on a commercial ion‐trap mass spectrometer and used to optimize mass spectrometric conditions for DNA nucleobases: adenine, cytosine, thymine, and guanine. Experimental parameters including spray voltage, distance between mass spectrometer inlet and the sampled spot, and nebulizing gas inlet pressure were optimized. Cluster ions including some magic number clusters of nucleobases were observed for the first time using DESI mass spectrometry. The formation of the cluster species was found to vary with the nucleobases, acidification of the spray solvent, and the deposited sample amount. All the experimental results can be explained well using a liquid film model based on the two‐step droplet pick‐up mechanism. It is further suggested that solubility of the analytes in the spray solvent is an important factor to consider for their studies by using DESI. Copyright © 2009 John Wiley & Sons, Ltd.
A longitudinal examination of the work–nonwork boundary strength construct
Authors: Hecht Tracy D. , Allen Natalie J. 
Published: January 2009

AbstractMany organizations are blurring the boundaries between work and nonwork through practices such as flextime, telecommuting, and on‐site day‐cares. Such integration of work and nonwork is purported to help employees find the seemingly elusive “work‐life balance.” Scholarly investigations of this issue have increased in number, but a standard measure of work–nonwork boundary strength has yet to emerge. The purpose of this research is to explore the boundary strength construct through the process of measure validation. In Study 1, data were collected from students (N = 162) to pilot test the measure. Study 2 was a longitudinal field study in which data were collected from employees of Canadian organizations (Survey 1: N = 793; Matched data for Surveys 1 & 2: N = 205). Confirmatory factor analyses supported the hypothesized two‐factor structure of the work–nonwork boundary strength measure, confirming the importance of differentiating boundary strength at home (BSH) and boundary strength at work (BSW). Longitudinal analyses confirmed the structural invariance of the measure and revealed that boundary strengths are relatively stable over a period of 1 year. Role identification was related to boundary strength at home only. Weak boundaries, both at home and at work, were associated with high inter‐role conflict. Copyright © 2009 John Wiley & Sons, Ltd.
How changes in job demands and resources predict burnout, work engagement, and sickness absenteeism
Authors: Schaufeli Wilmar B. , Bakker Arnold B. , Van Rhenen Willem 
Published: January 2009

AbstractThe present longitudinal survey among 201 telecom managers supports the Job Demands‐Resources (JD‐R) model that postulates a health impairment process and a motivational process. As hypothesized, results of structural equation modeling analyses revealed that: (1) increases in job demands (i.e., overload, emotional demands, and work‐home interference) and decreases in job resources (i.e., social support, autonomy, opportunities to learn, and feedback) predict burnout, (2) increases in job resources predict work engagement, and (3) burnout (positively) and engagement (negatively) predict registered sickness duration (“involuntary” absence) and frequency (“involuntary” absence), respectively. Finally, consistent with predictions results suggest a positive gain spiral: initial work engagement predicts an increase in job resources, which, in its turn, further increases work engagement. Copyright © 2009 John Wiley & Sons, Ltd.
Validation of green‐solvent extraction combined with chromatographic chemical fingerprint to evaluate quality of Stevia rebaudiana Bertoni
Authors: Teo Chin Chye , Tan Swee Ngin , Yong Jean Wan Hong , Hew Choy Sin , Ong Eng Shi 
Published: January 2009

AbstractAn approach that combined green‐solvent methods of extraction with chromatographic chemical fingerprint and pattern recognition tools such as principal component analysis (PCA) was used to evaluate the quality of medicinal plants. Pressurized hot water extraction (PHWE) and microwave‐assisted extraction (MAE) were used and their extraction efficiencies to extract two bioactive compounds, namely stevioside (SV) and rebaudioside A (RA), from Stevia rebaudiana Bertoni (SB) under different cultivation conditions were compared. The proposed methods showed that SV and RA could be extracted from SB using pure water under optimized conditions. The extraction efficiency of the methods was observed to be higher or comparable to heating under reflux with water. The method precision (RSD, n = 6) was found to vary from 1.91 to 2.86% for the two different methods on different days. Compared to PHWE, MAE has higher extraction efficiency with shorter extraction time. MAE was also found to extract more chemical constituents and provide distinctive chemical fingerprints for quality control purposes. Thus, a combination of MAE with chromatographic chemical fingerprints and PCA provided a simple and rapid approach for the comparison and classification of medicinal plants from different growth conditions. Hence, the current work highlighted the importance of extraction method in chemical fingerprinting for the classification of medicinal plants from different cultivation conditions with the aid of pattern recognition tools used.
Candidate's Thesis: Laryngotracheal separation in neurologically impaired children: Long‐term results
Authors: Cook Steven P. 
Published: January 2009

AbstractObjectives:Fifty‐six consecutive neurologically impaired pediatric children underwent laryngotracheal separation (LTS) for acute recurrent and chronic aspiration in the last 18 years. The population demographics, indications for surgery, and comorbidities are reviewed. This study reports early and late complications and survivorship including admissions for pneumonia/aspiration. Diagnosis related group (DRGs) and work relative value units (wRVUs) were measured to document the potential benefits before and after LTS.Study Design:Retrospective review of patient charts and records in an electronic medical record during an 18 year period.Methods:Information was obtained by a chart review and utilization of the electronic medical record. Patient specific DRG and wRVU data on their hospitalizations and outpatient encounters at the Alfred I. duPont Hospital for Children of the Nemours Foundation and survival data were recorded. Data was analyzed using chi‐square analysis, a two‐tailedttest, and a Fisher's Exact test.Results:Laryngotracheal separation achieved complete control of aspiration in all the children. A significant reduction in the number of hospital admissions for pneumonias after surgery was noted. After LTS there was a reduced average number of DRGs per month (p < .001) as well as wRVUs. Transient fistula formation (11%) was the most common complication. No patient had his or her procedure reversed to date.Conclusion:Laryngotracheal separation is 100% effective in controlling aspiration in all of neurologically impaired children in this study, It is a valuable procedure to prolong the life of children who have intractable aspiration. After LTS, a decrease in DRGs and wRVUs reduces health care costs for these patients. Prior to LTS, all medical and surgical treatment options for aspiration should be discussed and considered, based on the extent of the child's underlying neurologic status, ability to verbally communicate, degree of upper airway obstruction, and hope of recovery of neurologic function. Laryngoscope, 2009
Utility of anti‐HSP 70, TNF‐α, ESR, antinuclear antibody, and antiphospholipid antibodies in the diagnosis and treatment of sudden sensorineural hearing loss
Authors: Süslü Nilda , Yılmaz Taner , Gürsel Bülent 
Published: January 2009

AbstractObjectives:To investigate the performance of various laboratory tests used for patients with sudden sensorineural hearing loss (SSNHL).Study Design:Prospective clinical trial.Methods:Thirty patients who presented with SSNHL and 30 healthy people with no cochleovestibular disorders were selected as study and control groups.The laboratory panel includes the following tests: anti‐HSP 70 antibody immunoassay, tumor necrosis factor‐alpha (TNF‐α), erythrocyte sedimentation rate (ESR), antinuclear antibody (ANA), and antiphospholipid antibodies. The study group was given corticosteroid therapy and separated into two groups: the corticosteroid responders and the corticosteroid nonresponders. In the follow‐up, repeat audiograms were evaluated to determine the response to treatment.Results:TNF‐α was found at lower titers in the study group when compared with the control group in contrast to other studies. Also, anti‐HSP 70 was not found in high titers in the study group. ANA and ESR were the two parameters that were significantly more positive in the study group compared with the control group.Conclusions:Because of the lack of association between a positive test and response to corticosteroid treatment, detection of the anti‐HSP 70 antibody, TNF‐α, ESR, and ANA, at present, do not offer clinically useful information in the treatment of SSNHL. Also, because of the lower titers of TNF‐α documented in patients with SSNHL, we do not recommend the use of specific TNF‐α inhibitors in SSNHL. Laryngoscope, 2009
Changing patterns of tracheotomy use in anterior skull base surgery with free tissue reconstruction
Authors: Myers Larry L. , Sumer Baran D. , Lowery Jamel E. , Truelson John M. , Leach Joseph L. , Sinard Robert J. 
Published: January 2009

AbstractObjective:To evaluate how changing patterns of tracheotomy use with free tissue reconstruction of the anterior skull base affect postoperative complications.Design:Retrospective comparison of clinical cohort to historic control group.Setting:Tertiary care medical center.Patients:We reviewed 201 patients with a diagnosis of malignant or locally aggressive benign neoplasms of the nasal cavity and paranasal sinuses between January 1993 and December 2006. Of these, we studied 33 consecutive patients that underwent ablative anterior skull base surgery reconstructed with free tissue transfers. We divided the study group into two cohorts: Group 1, patients treated from 1993–2000, and Group 2, patients treated from 2000–2006.Main Outcome Measures:Tracheotomy use and postoperative complications.Results:There were 16 patients in Group 1 and 17 patients in Group 2. There were 11 patients who received a tracheotomy in Group 1, and only 5 in Group 2 (P = .038). Five patients in either group experienced complications (P = 1.000). There was one case of meningitis in Group 1 and no cases in Group 2 (P = .485). There were two cases of pneumocephalus in Group 2 and none in Group 1 (P = .485) and no cases of tension pneumocephalus. Six patients in each group had recurrent disease (P = 1.000). There was no statistically significant difference in survival (P = .675).Conclusions:The decreased use of tracheotomy in patients undergoing free tissue transfer reconstruction of anterior skull base defects did not lead to an increase in complications. Routine use of tracheotomy in these patients is unnecessary and should be reserved for selected cases. Laryngoscope, 2009