bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic and epigenetic regulation in nuclear microenvironments for biological control in cancer
Authors: Stein Gary S. , Zaidi Sayyed K. , Stein Janet L. , Lian Jane B. , van Wijnen Andre J. , Montecino Martin , Young Daniel W. , Javed Amjad , Pratap Jitesh , Choi Je‐Yong , Ali Syed A. , Pande Sandhya , Hassan Mohammad Q. 
Published: July 2008

AbstractThe regulatory machinery that governs genetic and epigenetic control of gene expression is compartmentalized in nuclear microenvironments. Temporal and spatial parameters of regulatory complex organization and assembly are functionally linked to biological control and are compromised with the onset and progression of tumorigenesis providing a novel platform for cancer diagnosis and treatment. J. Cell. Biochem. 104: 2016–2026, 2008. © 2008 Wiley‐Liss, Inc.
Dexamethasone and corticosterone induce similar, but not identical, muscle wasting responses in cultured L6 and C2C12 myotubes
Authors: Menconi Michael , Gonnella Patricia , Petkova Victoria , Lecker Stewart , Hasselgren Per‐Olof 
Published: July 2008

AbstractDexamethasone‐treated L6 (a rat cell line) and C2C12 (a mouse cell line) myotubes are frequently used as in vitro models of muscle wasting. We compared the effects of different concentrations of dexamethasone and corticosterone (the naturally occurring glucocorticoid in rodents) on protein breakdown rates, myotube size, and atrogin‐1 and MuRF1 mRNA levels in the two cell lines. In addition, the expression of the glucocorticoid receptor (GR) and its role in glucocorticoid‐induced metabolic changes were determined. Treatment with dexamethasone or corticosterone resulted in dose‐dependent increases in protein degradation rates in both L6 and C2C12 myotubes accompanied by 25–30% reduction of myotube diameter. The same treatments increased atrogin‐1 mRNA levels in L6 and C2C12 myotubes but, surprisingly, upregulated the expression of MuRF1 in L6 myotubes only. Both cell types expressed the GR and treatment with dexamethasone or corticosterone downregulated total cellular GR levels while increasing nuclear translocation of the GR in both L6 and C2C12 myotubes. The GR antagonist RU38486 inhibited the dexamethasone‐ and corticosterone‐induced increases in atrogin‐1 and MuRF1 expression in L6 myotubes but not in C2C12 myotubes. Interestingly, RU38486 exerted agonist effects in the C2C12, but not in the L6 myotubes. The present results suggest that muscle wasting‐related responses to dexamethasone and corticosterone are similar, but not identical, in L6 and C2C12 myotubes. Most notably, the regulation by glucocorticoids of MuRF1 and the role of the GR may be different in the two cell lines. These differences need to be taken into account when cultured myotubes are used in future studies to further explore mechanisms of muscle wasting. J. Cell. Biochem. 105: 353–364, 2008. © 2008 Wiley‐Liss, Inc.
Sex steroid hormones induce acylation stimulating protein resistance in 3T3‐L1 adipocytes
Authors: Wen Yu , Wang HongWei , MacLaren Robin , Lu HuiLing , Hu Xiu‐Fen , Cianflone Katherine 
Published: July 2008

AbstractAcylation stimulating protein (ASP) stimulates triglyceride synthesis and glucose transport via its receptor C5L2. In human studies, ASP is increased in insulin resistant states such as obesity, diabetes, polycystic ovary syndrome and late pregnancy (the latter two associated with altered sex hormones). The aims were (i) to evaluate ASP response and C5L2 expression following treatment with sex steroid hormones and (ii) to identify mechanisms of ASP resistance using 3T3‐L1 adipocytes and preadipocytes. Overnight incubation with physiological progesterone (PROG) concentrations induced dose‐dependent inhibition of ASP‐stimulated glucose transport in adipocytes (188 ± 11% +ASP, 100 ± 4% control, 129 ± 18% to 85 ± 7% [ASP + PROG 10−8 to 10−6 M] and preadipocytes (263 ± 18% +ASP, 100 ± 3% control, 170 ± 11% to 167 ± 4% [ASP + PROG 10−8 to 10−6 M]), while estradiol and testosterone (TEST) were effective only at the highest concentration (10−6 M). In adipocytes, dose‐dependent maximal C5L2 mRNA decreases were 39–75% (P = 0.003), with decreased cell‐surface C5L2 of −22% and −27% (10−6 M PROG and TEST, respectively) with no change in preadipocytes. Adipocytes treated with PROG displayed decreases in G proteins: Gβ (−55%), Gαq/11 (−56%) as well as complete inhibition of ASP stimulation. PROG significantly decreased basal levels of phosphorylated PKCα (p‐PKCα) while there was no change in p‐ PKCζ. ASP increased p‐PKCα and PKCζ to 161% (P < 0.0.001) and 160% (P < 0.01), a stimulation effectively blocked by PROG (10−8 and 10−6 M) and TEST (10−6 M). Sex steroid hormone‐induced ASP resistance via C5L2 may contribute to altered adipose tissue function and insulin resistance phenotype in humans. J. Cell. Biochem. 105: 404–413, 2008. © 2008 Wiley‐Liss, Inc.
Glycogen synthase kinase‐3β regulates ΔNp63 gene transcription through the β‐catenin signaling pathway
Authors: Chu Wing‐Keung , Dai Pei‐Min , Li Hsin‐Lun , Chen Jan‐Kan 
Published: July 2008

AbstractOverexpression of ΔNp63 has been observed in a number of human cancers, suggesting a role for ΔNp63 in carcinogenesis. In the present study, we show that inhibition of glycogen synthase kinase‐3β (GSK‐3β) by lithium chloride (LiCl) elicited a stimulatory effect on ΔNp63 promoter activity in HEK 293T cells. Exposure to LiCl induced ΔNp63 promoter activation as well as ΔNp63 protein expression in the cells. The effect of GSK‐3β on ΔNp63 expression was further confirmed by the use of two highly specific GSK‐3β inhibitors, SB216763 and SB415286. Further study showed the presence of a putative β‐catenin responsive element (β‐catenin‐RE) in the ΔNp63 promoter region, and the stimulation of ΔNp63 promoter activity by GSK‐3β inhibitor is markedly abolished by mutation or deletion of the putative β‐catenin‐RE. Data are also presented to show that β‐catenin acts together with Lef‐1 to influence ΔNp63 promoter activity and protein expression. J. Cell. Biochem. 105: 447–453, 2008. © 2008 Wiley‐Liss, Inc.
Wnt5a induces homodimerization and activation of Ror2 receptor tyrosine kinase
Authors: Liu Yan , Rubin Boris , Bodine Peter V.N. , Billiard Julia 
Published: July 2008

AbstractWnts are secreted glycoproteins that control vital biological processes, including embryogenesis, organogenesis and tumorigenesis. Wnts are classified into several subfamilies depending on the signaling pathways they activate, with the canonical subfamily activating the Wnt/β‐catenin pathway and the non‐canonical subfamily activating a variety of other pathways, including the Wnt/calcium signaling and the small GTPase/c‐Jun NH2‐terminal kinase pathway. Wnts bind to a membrane receptor Frizzled and a co‐receptor, the low‐density lipoprotein receptor related protein. More recently, both canonical and non‐canonical Wnts were shown to bind the Ror2 receptor tyrosine kinase. Ror2 is an orphan receptor that plays crucial roles in skeletal morphogenesis and promotes osteoblast differentiation and bone formation. Here we examine the effects of a canonical Wnt3a and a non‐canonical Wnt5a on the signaling of the Ror2 receptor. We demonstrate that even though both Wnt5a and Wnt3a bound Ror2, only Wnt5a induced Ror2 homo‐dimerization and tyrosine phosphorylation in U2OS human osteoblastic cells. Furthermore, Wnt5a treatment also resulted in increased phosphorylation of the Ror2 substrate, 14‐3‐3β scaffold protein, indicating that Wnt5a binding causes activation of the Ror2 signaling cascade. Functionally, Wnt5a recapitulated the Ror2 activation phenotype, enhancing bone formation in the mouse calvarial bone explant cultures and potentiating osteoblastic differentiation of human mesenchymal stem cells. The effect of Wnt5a on osteoblastic differentiation was largely abolished upon Ror2 down‐regulation. Thus we show that Wnt5a activates the classical receptor tyrosine kinase signaling cascade through the Ror2 receptor in cells of osteoblastic origin. J. Cell. Biochem. 105: 497–502, 2008. © 2008 Wiley‐Liss, Inc.
Global dynamics and transition state theories: Comparative study of reaction rate constants for gas‐phase chemical reactions
Authors: Ju Li‐Ping , Han Ke‐Li , Zhang John Z. H. 
Published: July 2008

AbstractIn this review article, we present a systematic comparison of the theoretical rate constants for a range of bimolecular reactions that are calculated by using three different classes of theoretical methods: quantum dynamics (QD), quasi‐classical trajectory (QCT), and transition state theory (TST) approaches. The study shows that the difference of rate constants between TST results and those of the global dynamics methods (QD and QCT) are seen to be related to a number of factors including the number of degrees‐of‐freedom (DOF), the density of states at transition state (TS), etc. For reactions with more DOF and higher density of states at the TS, it is found that the rate constants from TST calculations are systematically higher than those obtained from global dynamics calculations, indicating large recrossing effect for these systems. The physical insight of this phenomenon is elucidated in the present review. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
Dispersion in the Mott insulator UO2: A comparison of photoemission spectroscopy and screened hybrid density functional theory
Authors: Roy Lindsay E. , Durakiewicz Tomasz , Martin Richard L. , Peralta Juan E. , Scuseria Gustavo E. , Olson Cliff G. , Joyce John J. , Guziewicz Ela 
Published: July 2008

AbstractWe present a comparison between the screened hybrid density functional theory of Heyd, Scuseria, and Enzerhof (HSE06) and high‐resolution photoemission (PES) measurement on a single crystal of UO2. Angle‐resolved photoemission data show a slight dispersion in the f‐orbital derived bands in good agreement with the HSE band structure. The effect of spin‐orbit coupling on the HSE band gap has also been calculated and found to be negligible. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2008
Modeling the structure‐property relationships of nanoneedles: A journey toward nanomedicine
Authors: Poater Albert , Saliner Ana Gallegos , Carbó‐Dorca Ramon , Poater Jordi , Solà Miquel , Cavallo Luigi , Worth Andrew P. 
Published: July 2008

AbstractInnovative biomedical techniques operational at the nanoscale level are being developed in therapeutics, including advanced drug delivery systems and targeted nanotherapy. Ultrathin needles provide a low invasive and highly selective means for molecular delivery and cell manipulation. This article studies the geometry and the stability of a family of packed carbon nanoneedles (CNNs) formed by units of 4, 6, and 8 carbons, by using quantum chemistry computational modeling methods. At the limit of infinite‐length, these CNNs might act as semiconductors, especially when the number of terminal units is increased. CNNs are also potentially able to stabilize ions around their structure. Therefore, due to the apolar characteristics of CNNs and their ability to carry ionic species, they would be suitable to act as drug carriers through nonpolar biologic media. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
A novel method for enzyme design
Authors: Zhu Xiaolei , Lai Luhua 
Published: July 2008

AbstractRational design of enzymes is a stringent test of our understanding of protein structure and function relationship, which also has numerous potential applications. We present a novel method for enzyme design that can find good candidate protein scaffolds in a protein‐ligand database based on vector matching of key residues. Residues in the vicinity of the active site were also compared according to a similarity score between the scaffold protein and the target enzyme. Suitable scaffold proteins were selected, and the side chains of residues around the active sites were rebuilt using a previously developed side‐chain packing program. Triose phosphate isomerase (TIM) was used as a validation test for enzyme design. Selected scaffold proteins were found to accommodate the enzyme active sites and successfully form a good transition state complex. This method overcomes the limitations of the current enzyme design methods that use limited number of protein scaffold and based on the position of ligands. As there are a large number of protein scaffolds available in the Protein Data Band, this method should be widely applicable for various types of enzyme design. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
Accelerating molecular dynamic simulation on the cell processor and Playstation 3
Authors: Luttmann Edgar , Ensign Daniel L. , Vaidyanathan Vishal , Houston Mike , Rimon Noam , Øland Jeppe , Jayachandran Guha , Friedrichs Mark , Pande Vijay S. 
Published: July 2008

AbstractImplementation of molecular dynamics (MD) calculations on novel architectures will vastly increase its power to calculate the physical properties of complex systems. Herein, we detail algorithmic advances developed to accelerate MD simulations on the Cell processor, a commodity processor found in PlayStation 3 (PS3). In particular, we discuss issues regarding memory accessversuscomputation and the types of calculations which are best suited for streaming processors such as the Cell, focusing on implicit solvation models. We conclude with a comparison of improved performance on the PS3's Cell processor over more traditional processors. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
Scientific method, abduction, and clinical reasoning
Authors: Haig Brian D. 
Published: July 2008

AbstractThis special issue of the Journal of Clinical Psychology comprises six theoretical papers that are concerned with the interconnected topics of scientific method, abductive inference, and clinical reasoning. The first four papers deal with the nature and limitations of a broad abductive theory of scientific method, and its application to clinical reasoning and case formulation. These are followed by three papers which in turn consider the prospects of using explanatory criteria to appraise competing models of psychopathy, examine the merits of a number of different psychometric perspectives on the assessment of psychopathology, and reject a core supposition of the orthodox approach to hypothesis testing. © 2008 Wiley Periodicals, Inc. J Clin Psychol 64: 1–6, 2008.
Psychological services for returning veterans and their families: evolving conceptualizations of the sequelae of war‐zone experiences
Authors: Sammons Morgan T. , Batten Sonja V. 
Published: July 2008

AbstractThe provision of effective and timely behavioral health care for veterans returning from the conflicts in Iraq and Afghanistan has become the focus of national attention. In this special issue, attempts to provide psychological care for service members and their families are examined in light of three key constructs. First, it is contended that at no other time in history has more attention been paid to the psychological consequences of engaging in combat. Second, for the first time in recorded warfare, psychological morbidity is likely to far outstrip physical injury associated with combat. Finally, although posttraumatic stress disorder and traumatic brain injury are serious concerns, most service members return without significant physical or psychological injury and will be able to return to functioning without notable problems. Accurate diagnosis, a focus on resilience, and the expectation of readjustment are essential precepts that should guide clinical efforts and resource allocation. © 2008 Wiley Periodicals, Inc. J Clin Psychol: In Session 64:1–7, 2008.
Couple therapy with combat veterans and their partners
Authors: Erbes Christopher R. , Polusny Melissa A. , MacDermid Shelley , Compton Jill S. 
Published: July 2008

AbstractService members returning from Iraq and Afghanistan face psychological challenges that can exert profound effects on families and couples, but can also be treated within a systemic context. Couple therapy offers a means of increasing social support, decreasing interpersonal conflict, and addressing the experiential avoidance that maintains posttraumatic symptoms. For combat veterans and their partners, we present an adaptation of integrative behavioral couple therapy (IBCT) that reduces conflict and encourages intimacy through acceptance and skills strategies. By doing so, IBCT exposes service members in couple therapy to emotions, interpersonal situations, and activities that facilitate recovery from combat‐related distress. We illustrate common presenting problems in this population and the utilization of IBCT with a case example. © 2008 Wiley Periodicals, Inc. J Clin Psychol: In Session 64:1‐12, 2008.
Formation of Kv2.1‐FAK complex as a mechanism of FAK activation, cell polarization and enhanced motility
Authors: Wei Jian‐Feng , Wei Ling , Zhou Xin , Lu Zhong‐Yang , Francis Kevin , Hu Xin‐Yang , Liu Yu , Xiong Wen‐Cheng , Zhang Xiao , Banik Naren L. , Zheng Shu‐Sen , Yu Shan Ping 
Published: July 2008

AbstractFocal adhesion kinase (FAK) plays key roles in cell adhesion and migration. We now report that the delayed rectifier Kv2.1 potassium channel, through its LD‐like motif in N‐terminus, may interact with FAK and enhance phosphorylation of FAK397 and FAK576/577. Overlapping distribution of Kv2.1 and FAK was observed on soma and proximal dendrites of cortical neurons. FAK expression promotes a polarized membrane distribution of the Kv2.1 channel. In Kv2.1‐transfected CHO cells, formation of the Kv2.1‐FAK complex was stimulated by fibronectin/integrin and inhibited by the K+ channel blocker tetraethylammonium (TEA). FAK phosphorylation was minimized by shRNA knockdown of the Kv2.1 channel, point mutations of the N‐terminus, and TEA, respectively. Cell migration morphology was altered by Kv2.1 knockdown or TEA, hindering cell migration activity. In wound healing tests in vitro and a traumatic injury animal model, Kv2.1 expression and co‐localization of Kv2.1 and FAK significantly enhanced directional cell migration and wound closure. It is suggested that the Kv2.1 channel may function as a promoting signal for FAK activation and cell motility. J. Cell. Physiol. 217: 544–557, 2008. © 2008 Wiley‐Liss, Inc.
Induction of serum‐ and glucocorticoid‐induced kinase‐1 (SGK1) by cAMP regulates increases in α‐ENaC
Authors: Vasquez Margarita M. , Castro Robert , Seidner Steven R. , Henson Barbara M. , Ashton Daniel J. , Mustafa Shamimunisa B. 
Published: July 2008

Abstractα‐ENaC expression and activity is regulated by a variety of hormones including β‐adrenergic agonists via the second messenger cAMP. We evaluated the early intermediate pathways involved in the up‐regulation of SGK1 by DbcAMP and whether SGK1 is a prerequisite for induction of α‐ENaC expression. Submandibular gland epithelial (SMG‐C6) cells treated with DbcAMP (1 mM) induced both SGK1 mRNA and protein expression. DbcAMP‐stimulated SGK1 mRNA expression was decreased by actinomycin D and mRNA and protein expressions were attenuated by PKA inhibitors (H‐89 and KT5720). Inhibition of PI3‐K with either LY294002 or dominant negative PI3‐K reduced DbcAMP‐stimulated SGK1 protein and mRNA levels, attenuated the phosphorylation of CREB (a cAMP‐activated transcription factor) and decreased α‐ENaC protein levels and Na+ transport. In addition, the combination of PKA inhibitors with dominant negative PI3‐K synergistically inhibited DbcAMP‐induced Na+ transport. Inhibition of SGK1 expression by siRNA decreased but did not obliterate DbcAMP‐induced α‐ENaC expression. Thus, in a cell line which endogenously exhibits minimal α‐ENaC expression, induction of SGK1 by DbcAMP occurs via the PI3‐K and PKA pathways. Increased α‐ENaC levels and function are partly dependent upon the early induction of SGK1 expression. J. Cell. Physiol. 217: 632–642, 2008. © 2008 Wiley‐Liss, Inc.
Selective iron removal from spent passivation baths by ion exchange
Authors: Fernández‐Olmo I , Ortiz A , Urtiaga A , Ortiz I 
Published: July 2008

AbstractBACKGROUND: Post‐treatment of zinc plated surfaces is required to provide corrosion protection; for this purpose chromium passivation is usually used. In the EU, the older hexavalent chromium passivation baths have been replaced by trivalent chromium conversion coatings. However, the life of Cr(III) passivation baths is reduced due to iron and zinc impurities generated in the process. Thus, the objective of this work was to investigate the regeneration of spent passivation baths from the plating industry by ion exchange using a new chelating resin (Purolite S‐957).RESULTS: Three genuine passivation baths with different iron and zinc concentrations were investigated; the effect of loading flow rate, temperature and resin regeneration conditions on the uptake of iron ions was also studied. The removal performance of iron was increased at higher temperature (55 °C) and lower flow rates. The best regeneration conditions were found to be 6 bed volumes (BV) of 30 wt% HCl.CONCLUSION: This work has shown that iron can be selectively removed from trivalent chromium passivation baths using a new chelating resin (Purolite S‐957) and no addition of chromium (III) is required. Low feed flow rate and high bath temperature (up to 55 °C) are recommended for high iron uptake. Copyright © 2008 Society of Chemical Industry
Prenatal diagnosis of ectopic intrathoracic kidney in a fetus with a left diaphragmatic hernia
Authors: Panda Britta , Rosenberg Victor , Cornfeld Daniel , Stiller Robert 
Published: July 2008

AbstractIntrathoracic renal ectopia as a result of a congenital diaphragmatic hernia (CDH) is a rare congenital anomaly. We present a case in which the prenatal diagnosis of an ectopic intrathoracic kidney was made on routine anatomical survey at 28 weeks' gestation. Color doppler sonography imaging revealed the renal artery coursing into the infant's thorax and was consistent with CDH, but fetal MRI suggested an intact diaphragm. However, neonatal evaluation confirmed the diagnosis of intrathoracic kidney with posterior CDH, which was repaired without complication. In contrast to diaphragmatic hernia with liver or bowel herniation, infants with intrathoracic ectopic kidneys generally do well. © 2008 Wiley Periodicals, Inc. J Clin Ultrasound, 2009