bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of adipose—derived stromal vascular fraction in treatment of osteoarthritis: an experimental study
Authors: Moussa Sherine Alaa El Din Mohamed , Zaki M. Gamal El Din , Mohamed Manal Osman , Abo Zeid Asmaa A , Farrag Dina A. 
Published: March 2024

Abstract
Background
Osteoarthritis OA is a common progressive disabling disease. Current research aims at finding therapies to prevent its progression. In this work, we assessed the therapeutic role of intra-articular injection of stromal vascular fraction SVF in collagenase induced knee OA in rats.

Results
Post right Knee OA induction in 42 Wistar rats, histopathological examination and quantification of articular cartilage degeneration using Mankin’s score revealed degenerative changes were significantly higher in untreated Group II compared to SVF treated Group III at 1 month (10.75 ± 0.50 and 2.50 ± 0.53, P = 0.001) and 2 months (8.50 ± 0.58, 0.50 ± 0.53, P = 0.001), respectively. Morphometric computerized image analysis revealed a significant difference between treated, untreated and healthy control group I regarding chondrocyte cellular count, articular cartilage thickness and optical density OD of the cartilage (P < 0.001). Group II contained the least chondrocyte cellular count. Also, articular cartilage thickness at 2 months was significantly less in Group II compared to SVF treated group (P < 0.001). The OD in Safranin-stained slides, as an indicator of proteoglycan content of the matrix, was highest in Group I followed by Group III and lowest in Group II with a highly significant difference between untreated and treated groups at 1 month (67.32 ± 4.25, 81.77 ± 3.09, P = 0.000) and 2 months (71.60 ± 3.49, 83.26 ± 5.47, P = 0.000), respectively.

Conclusion
Treatment with adipose-derived SVF decreased the development of articular cartilage degenerative changes at early stages of induced OA in rats. Later, on follow-up, the preserved articular cartilage thickness, cellular count and increased proteoglycan content rendered SVF a promising regenerative therapy for Knee OA.
Plant genome editing to achieve food and nutrient security
Authors: Ceasar Stanislaus Antony , Kavas Musa 
Published: March 2024

AbstractGenome editing enables precise genetic manipulation in plants, offering hope for tackling global food insecurity and malnutrition by enhancing crop traits and nutritional content. The BMC Methods Collection ‘Genome Editing in Plants’ will showcase advancements in the field, including target selection, delivery systems, off-target effects, and efficiency optimization.
Normal fault reactivation induced by hydraulic fracturing: Poroelastic effects
Authors: Haddad Mahdi , Eichhubl Peter 
Published: March 2024

Numerous surface-felt earthquakes have been spatiotemporally correlated with hydraulic-fracturing operations. Because large deformations occur close to hydraulic fractures (HFs), any associated fault reactivation and resulting seismicity must be evaluated within the length scale of the fracture stages and based on the precise fault location relative to the simulated rock volumes. To evaluate the changes in Coulomb failure stress (CFS) with injection, we conduct fully coupled poroelastic finite-element simulations using a pore-pressure cohesive zone model for the fracture and fault core in combination with a fault-fracture intersection model. The simulations quantify the dependence of CFS and the fault reactivation potential on the host rock and fault properties, spacing between the fault and the HF, and the fracturing sequence. We find that fracturing in an anisotropic in-situ stress state does not lead to fault tensile opening but rather dominant shear reactivation through a poroelastic stress disturbance over the fault core ahead of the compressed central stabilized zone. In our simulations, poroelastic stress changes significantly affect fault reactivation in all the simulated scenarios of fracturing 50–200 m away from an optimally oriented normal fault. Asymmetric HF growth due to the stress shadowing effect of the adjacent HFs leads to (1) a larger reactivated fault zone following the simultaneous and sequential fracturing of multiple clusters compared with single-cluster fracturing and (2) a larger unstable area ([Formula: see text].1) over the fault core or a higher potential of the fault slip following sequential fracturing compared with simultaneous fracturing. The fault reactivation area is further increased for a fault with lower conductivity and a higher opening-mode fracture toughness of the overlying layer. To reduce the risk of fault reactivation by hydraulic fracturing under the reservoir characteristics of the Barnett Shale, Fort Worth Basin, it is recommended to (1) conduct simultaneous fracturing instead of sequential and (2) maintain a minimum distance of approximately 200 m for HF operations from known faults.
Living Arrangements: Mise-en-scène and the Nouveau Roman in the Fiction of Ann Quin
Authors: Matore Daniel 
Published: March 2024

Abstract
Why is postwar experimental fiction so concerned with measuring and marking out scene and setting? This article analyzes the fiction of the avant-garde British novelist Ann Quin, both her short stories and her debut novel Berg, focusing on her mise-en-scène, choreography, and tableaux. It argues for a fraught relationship between twentieth-century fiction and setting, from Henry James’s “house of fiction” and Woolf’s denunciation of naturalist interiors, from the self-sufficient psyches of early modernism to the fastidious mapping of scene and place in the theory of the nouveau roman, especially that of Natalie Sarraute and Alain Robbe-Grillet. The importance of Quin’s work for postwar fiction, the article argues, is that it suspends, periodically, the free indirect style of early modernism but also departs from the austere topography of the nouveau roman, so that her fiction furnishes an elastic, anxious, and unstable mise-en-scène.
Opening the Black Box of Implementation: Developing the Creating Active Schools Logic Model
Authors: Helme Zoe E. , Chalkley Anna , Walker Timothy J. , Bartholomew John B. , Morris Jade L. , McLoughlin Gabriella M. , Bingham Daniel D. , Daly-Smith Andy 
Published: March 2024

ABSTRACT
Global guidance is driving systems thinking to the forefront of research, policy, and practice. To achieve this, we need to consider how things work to ensure successful implementation. The use of the implementation research logic model as a contemporary tool to aid the planning, reporting, synthesizing, executing, and evaluating of the novel Creating Active Schools (CAS) program is illustrated. A five-step iterative process, underpinned by the implementation research logic model, was undertaken to hypothesize and identify conceptual pathways between the CAS: 1) program determinants, 2) program components and actions, 3) implementation strategies, 4) mechanisms of action, and 5) outcomes. Throughout development, school-based and CAS stakeholders engaged in reviewing appropriate implementation theories, models and frameworks, terminology, and content. An in-depth CAS logic model was created to detail the CAS program and underpinning mechanisms. This article provides novel insights into how contemporary implementation tools can be applied and adapted to enhance the planning, reporting, synthesizing, executing, and evaluating of complex interventions and strategies. The CAS logic model provides a blueprint for future school-based interventions to develop evidence-based logic models and to increase the likelihood of acceptance, feasibility, and sustainability.
FATTY ACID CONTENT AND MORPHOLOGICAL INDICATORS OF WINTER WHEAT (TRITICUM AESTIVUM L.) SEEDLINGS GROWN FROM SEEDS TREATED WITH OZONE
Authors: Kapustina Irina S. , Lazukin Alexander V. , Nurminsky Vadim N. , Grabelnykh Olga I. , Ozolina Natalia V. , Gurina Veronika V. , Spiridonova Ekaterina V. 
Published: March 2024

Background. Presently, a selection of environmentally friendly technologies for pre-sowing treatment of wheat seeds is conducted in agriculture. Ozone, an allotropic form of oxygen, is highly reactive, acts as an effective insecticide, promotes seed improvement, and reduces soil contamination. The impact of ozone on seeds is one of the promising technologies to improve the quality of seedlings. It is known that the field germination of winter wheat may be less than 50%, depending on the environmental conditions, and therefore the quality of seedlings is very important.
Purpose. To evaluate the effect of different ozone concentrations on morphological parameters and synthesis of fatty acids (FA) in winter wheat seedlings.
Materials and methods. The object of the study was the seeds of soft winter wheat (variety Irkutskaya). The ozonation was conducted with ozone concentrations of 2, 4, 6 and 8 g/m3 during 15, 30, 45 and 60 min. The germination of treated and control seeds was carried out for three days in the dark in a thermostat (24±1°C). On the third day, the morphometric characteristics were measured. The extraction of lipids from shoots and roots was carried out according to the method of Bligh, Dyer [14]. The methyl esters of lipid fatty acids were analyzed by chromato-mass spectrometry. Statistical data processing was conducted with the use of R programming language and SigmaPlot v. 12.5.
Results. Ozone in concentrations of 2, 4, 6 g/m3 has a stimulating effect on seedling germination, the length of the shoots and roots increases. At ozone concentration of 8 g/m3, the length of the shoots and roots decreases.
There is a general trend in the content of fatty acids, in the shoots of seedlings there is some decrease in lipid unsaturation, and in the roots - some increase in lipid unsaturation.
Conclusion. Ozone in concentrations up to 8 g/m3 is suitable for pre-sowing seed treatment and improves seedling performance.
HYGIENIC ASSESSMENT OF THE IMPACT OF OFF-GRID HEAT SOURCES ON AMBIENT AIR QUALITY AND THE FORMATION OF PUBLIC HEALTH RISKS
Authors: Zaitseva Nina V. , Kleyn Svetlana V. , Andrishunas Alena M. , Balashov Stanislav Yu. , Chigvintsev Vladimir M. 
Published: March 2024

Background. The study is relevant due to a significant number (more than 74 thousand) of autonomous heat sources (AHS) in the Russian Federation, especially in its eastern regions. Such sources use solid fuels (coal). AHS, along with other heat and power objects, make a considerable contribution to ambient air pollution in the ground layers and consequently produce harmful effects on public health.
The aim of this study was to perform hygienic assessment of negative effects produced by autonomous heat sources (AHS) on ambient air quality, health risks and additional associated incidence on the example of a territory included into the Clean Air Federal project.
Materials and methods. We calculated dispersion of pollutants in ambient air in residential areas due to AHS; performed hygienic assessment of calculated and instrumental data on ambient air quality; assessed public health risks; identified the number of additional associated diseases in accordance with the conventional established methods and algorithms valid at the present moment.
Results. More than 170 AHS are located on the analyzed territory; they all use soli fuel (coal). Hygienic standards are violated in areas influenced by AHS for four out of sever chemicals emitted by chimneys of private houses. Pollutant levels reach 5.1 single maximum MPC for four pollutants according to dispersion calculation; 4.8 single maximum MPC, 38 average daily MPC and 4.7 average annual MPC for four pollutants according to instrumental data; 29.9 single maximum MPC, 3.5 average daily MPC and 7.0 average annual MPC for three pollutants according to verified data. Identified carcinogenic, acute, and chronic health risks are ranked as ‘alerting’ and ‘high’ in areas under direct influence of AHS; these risks reach 13.4 HQ (acute), 7.0 HQ (chronic), CRT 1.19∙10-7 (carcinogenic). AHS create more than 11 thousand additional respiratory diseases (6,379.9 cases per 100 thousand people); of them, 98% cases are registered in children.
Conclusion. AHS create high levels of exposure to harmful pollutants and elevated health risks as well as additional incidence associated with ambient air quality. This situation requires immediate attention of the local authorities, control and surveillance organs; it is necessary to develop and implement complex air protection activities as soon as possible.
SAFETY OF MILK AND MEAT PRODUCTS IN THE RUSSIAN FEDERATION IN DYNAMICS: RESULTS OF SANITARY-EPIDEMIOLOGICAL CONTROL (2013-2022)
Authors: May Irina V. , Nikiforova Nadezhda V. 
Published: March 2024

Background. The study addresses an extremely important issue, namely quality and safety of meat and milk products that are consumed daily by majority of the population in the Russian Federation.
Aim. To investigate safety of animal food products (meat and milk products) in dynamics.
Materials and methods. We performed retrospective analysis of the branch statistical report with the results of control (surveillance) over milk and meat products obtained in the period between 2013 and 2022. We estimated safety levels in dynamics and spatial distribution of shares of unsafe goods as per priority indicators.
Results. Frequency of cases when mandatory requirements to safety of milk and meat products were violated amounted to 4.0 % and approximately 3.0 % accordingly. Hygienic requirements were most frequently violated as per microbiological and physical-chemical indicators, 3.84 % and 2.69 % accordingly for meat products and 4.2 % and 6.0 % accordingly for milk products. We analyzed safety of meat products in dynamics and established positive trends to be rather weak and multidirectional as per different indicators; as for milk products, shares of unsafe products went down as per all the standardized indicators except from sanitary-chemical ones.
Conclusion. Our study didn’t reveal any obvious positive dynamics (descending trends) in the shares of food product samples deviating from the safety standards. This means it is necessary to improve the current system for control (surveillance) over meat and milk products. More effective control (surveillance) can be provided by creating ‘risk profiles’ of a product with information about the most risky indicators to describe hazards associated with it. These indicators should be the first to be included into programs of product laboratory testing. In addition, it is vital to create regional registers of food products assigned into specific categories as per associated health risks. Such registers can also be used to select groups and types of products that should be covered by surveillance. The development of the risk-based surveillance can facilitate elimination of low quality and unsafe food products form the market.
The Financial Consequences of Online Review Aggregators: Evidence from Yelp Ratings and SBA Loans
Authors: Huang Ruidi 
Published: March 2024

This paper demonstrates the financial and real consequences of online review aggregators. Exploiting a regression discontinuity design that overcomes the endogenous relationship between Yelp reviews and Small Business Administration loan outcomes, I show that higher coarse Yelp ratings lead to improved loan terms and performance. Specifically, a one-half-star increase in Yelp ratings corresponds to a 25-basis-point decrease in loan spread and 6% lower collateral requirements. The effects are more pronounced when banks have less borrower information. Higher Yelp ratings also contribute to increased consumer demand and the likelihood of future business openings. These findings indicate that online review aggregators influence both consumer choices and banks’ financing decisions. This paper was accepted by Gustavo Manso, finance. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2020.03003 .
Sudden visual loss due to arteritic anterior ischaemic optic neuropathy: A rare manifestation of eosinophilic granulomatosis with polyangiitis
Authors: Faraone Antonio , Fortini Alberto , Borgioli Vanni , Cappugi Chiara , Lo Forte Aldo , Bottaro Valeria Maria , Vaglio Augusto 
Published: March 2024

Background: eosinophilic granulomatosis with polyangiitis (EGPA) is a rare multisystem inflammatory disease characterized by asthma, eosinophilia and granulomatous or vasculitic involvement of various organs. While the eye is uncommonly affected in patients with EGPA, multiple ophthalmic manifestations have been reported, which can result in serious visual impairment without timely treatment. Case report: we report the case of a 79-year-old woman with a history of asthma and nasal polyps who presented with low-grade fever, mild alteration of mental status, and fatigue. Chest X-ray revealed bilateral interstitial infiltrates. Lab tests showed elevated C-reactive protein level and eosinophilia (eosinophil count, 4.6 x109 cells/l); blood cultures and parasitological examination of stools tested negative. Four days after presentation, the patient reported sudden and severe blurring of vision in her left eye. Ophthalmological examination revealed bilateral swollen optic disc and visual field loss, more severe in the left eye. A diagnosis of EGPA complicated by arteritic anterior ischaemic optic neuropathy (A-AION) was proposed, while an alternative or concurrent diagnosis of giant cell arteritis was ruled out based on clinical picture. Immunosuppressive treatment with high-dose intravenous glucocorticoids was promptly started. The patient’s visual defect did not improve; however, two months later, no worsening was registered on ophthalmic reassessment. Conclusions: A-AION is an infrequent but severe manifestation of EGPA, requiring prompt diagnosis and emergency-level glucocorticoid therapy to prevent any further vision loss. Disease awareness and a multidisciplinary approach are crucial to expedite diagnostic work-up and effective management of EGPA-related ocular complications.
Successful chemotherapy treatment for a tricuspid valvular stenosis patient due to right ventricular lymphoma
Authors: Chien Do Van , Quy Tran Quoc 
Published: March 2024

Background: This study presents a patient diagnosed with tricuspid valvular stenosis due to right ventricular lymphoma, who was treated successfully. Case presentation: A 66-year-old man with a history of worsening shortness of breath during activity for the last three weeks sought medical attention. The patient later experienced swelling in the extremities, fluid build-up around the lungs and abdominal fluid accumulation, with no reported chest pain, fever, or weight loss. An echocardiogram found a mass in the lateral wall near the tricuspid valve of the right ventricle, leading to moderate tricuspid stenosis. The cardiac magnetic resonance imaging (MRI) revealed a lumpy, poorly defined mass that invaded the heart muscle and displayed varied enhancement after contrast administration. Suspicion arose for a malignant tumour or metastatic lesion due to its features and contrast uptake capability. A percutaneous biopsy was carried out on the mass in the right ventricle to confirm the diagnosis. The pathology report indicated a diagnosis of non-Hodgkin’s lymphoma. After being diagnosed, the patient underwent chemotherapy using the R-CHOP regimen. Over time the symptoms improved, and echocardiograms revealed a decrease in the size of the tumour. After undergoing six rounds of chemotherapy, a cardiac MRI four months later showed no signs of a tumour. After that, the patient resumed their regular activities. Conclusion: Right ventricular tumours are mostly malignant lesions and often have an inferior prognosis. Early diagnosis with imaging techniques and myocardial biopsy is necessary to deliver life-saving treatment quickly.
Allogeneic mesenchymal stem cells may be a viable treatment modality in cerebral palsy
Authors: Boyalı Osman , Kabatas Serdar , Civelek Erdinç , Ozdemir Omer , Bahar-Ozdemir Yeliz , Kaplan Necati , Savrunlu Eyüp Can , Karaöz Erdal 
Published: March 2024

BACKGROUND
Cerebral palsy (CP) describes a group of disorders affecting movement, balance, and posture. Disturbances in motor functions constitute the main body of CP symptoms. These symptoms surface in early childhood and patients are affected for the rest of their lives. Currently, treatment involves various pharmacotherapies for different types of CP, including antiepileptics for epilepsy and Botox A for focal spasticity. However, none of these methods can provide full symptom relief. This has prompted researchers to look for new treatment modalities, one of which is mesenchymal stem cell therapy (MSCT). Despite being a promising tool and offering a wide array of possibilities, mesenchymal stem cells (MSCs) still need to be investigated for their efficacy and safety.
AIM
To analyze the efficacy and safety of MSCT in CP patients.
METHODS
Our sample consists of four CP patients who cannot stand or walk without external support. All of these cases received allogeneic MSCT six times as 1 × 106/kg intrathecally, intravenously, and intramuscularly using umbilical cord-derived MSCs (UC-MSC). We monitored and assessed the patients pre- and post-treatment using the Wee Functional Independence Measure (WeeFIM), Gross Motor Function Classification System (GMFCS), and Manual Ability Classification Scale (MACS) instruments. We utilized the Modified Ashworth Scale (MAS) to measure spasticity.
RESULTS
We found significant improvements in MAS scores after the intervention on both sides. Two months: Right χ 2 = 4000, P = 0.046, left χ 2 = 4000, P = 0.046; four months: Right χ 2 = 4000, P = 0.046, left χ 2 = 4000, P = 0.046; 12 months: Right χ 2 = 4000, P = 0.046, left χ 2 = 4000, P = 0.046. However, there was no significant difference in motor functions based on WeeFIM results (P > 0.05). GMFCS and MACS scores differed significantly at 12 months after the intervention (P = 0.046, P = 0.046). Finally, there was no significant change in cognitive functions (P > 0.05).
CONCLUSION
In light of our findings, we believe that UC-MSC therapy has a positive effect on spasticity, and it partially improves motor functions.
Construction of the underlying circRNA-miRNA-mRNA regulatory network and a new diagnostic model in ulcerative colitis by bioinformatics analysis
Authors: Yuan Yu-Yi , Wu Hui , Chen Qian-Yun , Fan Heng , Shuai Bo 
Published: March 2024

BACKGROUND
Circular RNAs (circRNAs) are involved in the pathogenesis of many diseases through competing endogenous RNA (ceRNA) regulatory mechanisms.
AIM
To investigate a circRNA-related ceRNA regulatory network and a new predictive model by circRNA to understand the diagnostic mechanism of circRNAs in ulcerative colitis (UC).
METHODS
We obtained gene expression profiles of circRNAs, miRNAs, and mRNAs in UC from the Gene Expression Omnibus dataset. The circRNA-miRNA-mRNA network was constructed based on circRNA-miRNA and miRNA-mRNA interactions. Functional enrichment analysis was performed to identify the biological mechanisms involved in circRNAs. We identified the most relevant differential circRNAs for diagnosing UC and constructed a new predictive nomogram, whose efficacy was tested with the C-index, receiver operating characteristic curve (ROC), and decision curve analysis (DCA).
RESULTS
A circRNA-miRNA-mRNA regulatory network was obtained, containing 12 circRNAs, three miRNAs, and 38 mRNAs. Two optimal prognostic-related differentially expressed circRNAs, hsa_circ_0085323 and hsa_circ_0036906, were included to construct a predictive nomogram. The model showed good discrimination, with a C-index of 1(> 0.9, high accuracy). ROC and DCA suggested that the nomogram had a beneficial diagnostic ability.
CONCLUSION
This novel predictive nomogram incorporating hsa_circ_0085323 and hsa_circ_0036906 can be conveniently used to predict the risk of UC. The circRNa-miRNA-mRNA network in UC could be more clinically significant.