bluuebunny/crossref_metadata_2025_split huggingface dataset:
HPLC‐F analysis of melatonin and resveratrol isomers in wine using an SPE procedure
Authors: Mercolini Laura , Addolorata Saracino Maria , Bugamelli Francesca , Ferranti Anna , Malaguti Marco , Hrelia Silvana , Raggi Maria Augusta 
Published: March 2008

AbstractAn original analytical method has been developed for the determination of the antioxidants trans‐resveratrol (t‐RSV) and cis‐resveratrol (c‐RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm×4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03–5.00 ng/mL for MLT, 3–500 ng/mL for t‐RSV and 1–150 ng/mL for c‐RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t‐RSV and 0.3 ng/mL for c‐RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.
New α‐amino phenylalanine tetrazole ligand for immobilized metal affinity chromatography of proteins
Authors: Lei Genhu , Liu Liting , Xiong Xiaohu , Wei Yinmao , Zheng Xiaohui 
Published: March 2008

AbstractA new chelating compound has been developed for use in the immobilized metal affinity chromatographic (IMAC) separation of proteins. The bidentate ligand, α‐amino phenylalanine tetrazole, 4, was synthesized via a five‐step synthesis from N‐fluorenylmethoxycarbonyl phenylalanine and then immobilized onto silica through the epoxide coupling procedure. The binding behavior of the resulting IMAC sorbent, following chelation with Zn2+ to a density of 183 μmol Zn2+ ions/g silica, was characterized by the retention of proteins in the pH range of 5.0–8.0, and by the adsorption behavior of lysozyme with frontal chromatography at pH 6.0 and 8.0. The prepared column showed the separation ability to four test proteins and the retention time of these proteins increased with an increase in pH. From the derived isotherms, the adsorption capacity, qm, for the binding of lysozyme to immobilized Zn2+‐α‐amino phenylalanine tetrazole–silica was found to be 1.21 μmol/g at pH 6.0 and 1.20 μmol/g sorbent at pH 8.0, respectively, whilst the dissociation constants KD at these pH values were 5.22×10–6 and 3.49×10–6 M, respectively, indicating that the lysozyme was retained more stable under alkaline conditions, although the binding capacity in terms of micromole protein per gram sorbent remained essentially unchanged.
Through oven transfer adsorption–desorption interface for the analysis of methyl jasmonate in aromatic samples by on‐line RPLC‐GC
Authors: Flores Gema , Blanch Gracia Patricia , Ruiz del Castillo Maria Luisa 
Published: March 2008

AbstractA fully automated method for the determination of medium volatility compounds in aromatic samples was developed. Specifically, the determination of methyl jasmonate in jasmine fragrances was performed by using the through oven transfer adsorption–desorption (TOTAD) interface for the on‐line coupling between RPLC‐GC. A study of the most relevant variables involved in the performance of the TOTAD interface for medium volatility compounds was carried out by testing different values of helium flow (100, 300, 400, and 500 mL/min), transfer speed (0.1, 0.3, 0.5, and 2.0 mL/min), and methanol/water percentages (86:14, 85:15, 83:17, 80:20, and 70:30). The method developed provided satisfactory repeatability (RSD for retention times of 0.15% and for peak areas of 9.4%) and recovery (71%) as well as excellent LOD (0.01 mg/L) for methyl jasmonate in commercial jasmine essence under the experimental conditions selected as optimum. Additional advantages of the automated RPLC‐TOTAD‐GC method proposed in the present work are its rapidness, reliability, and the possibility of directly introducing the sample with no further pretreatment.
Central neurocytoma responsive to topotecan, ifosfamide, carboplatin
Authors: Amini Elham , Roffidal Tina , Lee Alice , Fuller Gregory N. , Mahajan Anita , Ketonen Leena , Kobrinsky Nathan , Cairo Mitchell S. , Wells Robert J. , Wolff Johannes E.A. 
Published: March 2008

AbstractA 5‐year‐old male presented with spinal cord drop metastasis from a recurrent neurocytoma. Topotecan (0.5 mg/m2) and carboplatin (250 mg/m2) were administered on days 1–3 and ifosfamide (1,800 mg/m2) on days 1–5, every 21 days, for three cycles and resulted in complete response without severe complications. A literature review yielded 20 patients with central neurocytoma but no complete responses. The complete response of central neurocytoma to chemotherapy only reported here should be helpful to those caring for patients with this rare tumor. Pediatr Blood Cancer 2008;51:137–140. © 2008 Wiley‐Liss, Inc.
Primhe: Primary Care Mental Health & Education
Authors: Sapper Helen 
Published: March 2008

AbstractSupport for migrant healthcare professionalsMigrant and Refugee Communities Forum (MRCF) is an umbrella organisation established to provide support and advice for migrants living in West London. Here, Helen Sapper explains how MRCF is providing support to migrant doctors and other healthcare professionals wishing to practice in the UK. Copyright © 2008 Wiley Interface Ltd
Fungicide seed treatment efficacy against Microdochium nivale and M. majus in vitro and in vivo
Authors: Glynn Neil C , Hare Martin C , Edwards Simon G 
Published: March 2008

AbstractBACKGROUND: Seed‐borne Microdochium majus (Wollenweber) and M. nivale Fries are the primary pathogens responsible for Fusarium seedling blight in the UK. The two species show differences in pathogenicity, host preference and sensitivities to temperature, but their relative sensitivities to fungicide seed treatments are unknown. The aim was firstly to determine the efficacy of fungicide seed treatments towards single‐spore isolates of M. majus and M. nivale using in vitro experiments, and subsequently to determine efficacy in vivo over a range of temperatures.RESULTS: Differences in EC50 values between all seed treatments were evident from the in vitro experiments and ranged from 0.028 mg L−1 for fludioxonil to 22.8 mg L−1 for carboxin + thiram. The two seed treatments that showed best performance in vitro were used to examine efficacy towards seed‐borne infection in vivo at 4, 8, 12 and 16 °C. Generally, seedling emergence improved and the severity of stem‐base disease symptoms on emerged seedlings was reduced for both species through the use of the fungicides. The combination of fludioxonil + difenconazole showed improved performance compared with fludioxonil alone. Significantly less severe symptoms were observed through the use of fludioxonil and fludioxonil + difenconazole compared with bitertanol + fuberidazole at 12 °C and for all except one M. nivale infected seed lot at 8 °C.CONCLUSIONS: Differences in fungicide sensitivity between the two species in vitro were not evident in vivo. This is the first report of the effect of fungicide seed treatments on the control of seedling blight caused by M. majus and M. nivale. Copyright © 2008 Society of Chemical Industry
MIA‐QSAR evaluation of a series of sulfonylurea herbicides
Authors: Bitencourt Michelle , Freitas Matheus P 
Published: March 2008

AbstractBACKGROUND: A series of sulfonylurea herbicides has been modelled using a 2D image‐based QSAR approach known as MIA‐QSAR (Multivariate Image Analysis applied to QSAR), and highly predictive models have been built.RESULTS: Two MIA‐QSAR models were built, one group being divided into training and test sets, and the other composed of the entire series of compounds. Statistically significant MIA‐QSAR models rendered high correlation coefficients of experimental versus fitted p$K_{\rm {i}}^{\rm {app}}$ (AHAS apparent inhibition constant) and satisfactory parameters of external validation and leave‐one‐out cross‐validation. Comparison with the results obtained from classical 2D QSAR demonstrated some advantages of the modelling using MIA descriptors.CONCLUSION: Both MIA‐QSAR models showed high predictive ability, comparable with that of a reference methodology based on 3D descriptors. The method is suggested as a suitable tool for predicting novel herbicides. Copyright © 2008 Society of Chemical Industry
Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis
Authors: Liu Yi , Zhang Shuyu , Ling Xiaomei , Li Yuzhen , Zhang Yingmei , Han Wenling , Wang Ying 
Published: March 2008

AbstractThe Chemokine‐like factor 1 (CKLF1) is a novel human cytokine and exhibits chemotactic activities on leukocytes. Two peptides named CKLF1‐C27 and CKLF1‐C19, were obtained from secreted CKLF1. In this study, a selective high‐performance analytical method based on capillary zone electrophoresis (CZE) to investigate interactions between heparin and CKLF1‐C27/CKLF1‐C19 was developed. Samples containing CKLF1‐C27/CKLF1‐C19 and heparin at various ratios were incubated at room temperature and then separated by CZE with Tris‐acetate buffer at pH 7.2. Both qualitative and quantitative characterizations of the binding were determined. The binding constants of the interactions between CKLF1‐C27/CKLF1‐C19 and heparin were calculated as (3.38 ± 0.49) × 105 M−1 and (1.10 ± 0.02) × 105 M−1 by Scatchard analysis. To study structural requirements, CKLF1‐C19pm and CKLF1‐C19km have been synthesized, and their interactions with heparin have been studied by CZE. We found that the Pro or Lys to Ala substitution within the residues of CKLF1‐C19 (CKLF1‐C19pm or CKLF1‐C19km) strongly decreased or abolished its interaction with heparin, suggesting that the residues of Pro affect the affinity of CKLF1‐C19 for heparin, and the residues of Lys of CKLF1‐C19 play the important role for the interaction of CKLF1‐C19 and heparin, respectively. The methodology presented should be generally applicable to study peptides and heparin interactions quantitatively and qualitatively. Copyright © 2008 European Peptide Society and John Wiley & Sons, Ltd.
Structures and energies of ArnH2O (n = 1–26) clusters using a nonrigid potential surface: A molecular dynamics simulation
Authors: Borges E. , Ferreira G. G. , Braga J. P. 
Published: March 2008

AbstractStable structures and minima energies of ArnH2O (n = 1–26) clusters are determined by carrying out a stochastic search method coupling to molecular dynamics calculations. A nonrigid intramolecular potential surface together with a pairwise‐additive intermolecular potential are used. A growing pattern mechanism is described for the structures in the ground state and a solvation process is observed. Molecular relaxation effects are characterized and the second differences on the cluster binding energies are calculated. The relative stability of the structures is discussed and compared with previous theoretical results. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Selective epitaxial growth of in situ carbon‐doped silicon on silicon substrates
Authors: Ikuta Tetsuya , Fujita Shigeru , Iwamoto Hayato , Kadomura Shingo , Shimura Takayoshi , Watanabe Heiji , Yasutake Kiyoshi 
Published: March 2008

AbstractThe characteristics of in situ carbon‐doped Si fabricated by selective epitaxial growth (SEG) were investigated. We confirmed the good selectivity, high crystalline quality, high strain and constant carbon profile of the film. The high ratio of the concentration of carbon atoms located at the substitutional sites to that of the total carbon atoms was achieved at low‐temperature SEG. Copyright © 2008 John Wiley & Sons, Ltd.
The use of hierarchical models for estimating relative risks of individual genetic variants: An application to a study of melanoma
Authors: Capanu Marinela , Orlow Irene , Berwick Marianne , Hummer Amanda J. , Thomas Duncan C. , Begg Colin B. 
Published: March 2008

AbstractFor major genes known to influence the risk of cancer, an important task is to determine the risks conferred by individual variants, so that one can appropriately counsel carriers of these mutations. This is a challenging task, since new mutations are continually being identified, and there is typically relatively little empirical evidence available about each individual mutation. Hierarchical modeling offers a natural strategy to leverage the collective evidence from these rare variants with sparse data. This can be accomplished when there are available higher‐level covariates that characterize the variants in terms of attributes that could distinguish their association with disease. In this article, we explore the use of hierarchical modeling for this purpose using data from a large population‐based study of the risks of melanoma conferred by variants in the CDKN2A gene. We employ both a pseudo‐likelihood approach and a Bayesian approach using Gibbs sampling. The results indicate that relative risk estimates tend to be primarily influenced by the individual case–control frequencies when several cases and/or controls are observed with the variant under study, but that relative risk estimates for variants with very sparse data are more influenced by the higher‐level covariate values, as one would expect. The analysis offers encouragement that we can draw strength from the aggregating power of hierarchical models to provide guidance to medical geneticists when they offer counseling to patients with rare or even hitherto unobserved variants. However, further research is needed to validate the application of asymptotic methods to such sparse data. Copyright © 2008 John Wiley & Sons, Ltd.
Iron valence states in organic samples and tissues investigated by XANES and Mössbauer spectroscopy
Authors: Dziedzic‐Kocurek K. , Banaś A. , Kwiatek W. M. , Stanek J. 
Published: March 2008

AbstractThe results of XANES and Mössbauer spectroscopy studies of organic samples of [Fe(Hka)3] (Hka = kojic acid) and [Fe(etmal)3] (etmal = ethyl maltol) are presented and discussed in terms of their application in characterization of the local electronic state of Fe3+. In case of [Fe(etmal)3], a tiny increase in covalency of iron bonds was found. The local iron states in tissues have been concluded from x‐ray absorption near‐edge structures (XANES) spectra, compared with spectra obtained for the organic samples. Significant increase of iron concentration in pathological cells of the prostate was shown by the synchrotron radiation‐induced x‐ray emission (SRIXE) method. XANES analysis of healthy and cancerous tissue samples suggested higher iron oxidation state in pathological cells. This result may be a hint for understanding the role of iron in carcinogenesisand opens up the same possibility of using XANES spectroscopy as a diagnostic tool in oncology. The correlation between results obtained by XANES and Mössbauer spectroscopy may be helpful in overcoming the limitations characteristic of both of the methods in the determination of the iron valence and spin states in tissues. Copyright © 2008 John Wiley & Sons, Ltd.
Simultaneous Use of Laparoscopy and Endoscopy for Minimally Invasive Resection of Gastric Subepithelial Masses — Analysis of 93 Interventions
Authors: Wilhelm D. , Delius S. v. , Burian M. , Schneider A. , Frimberger E. , Meining A. , Feussner H. 
Published: March 2008

AbstractBackgroundSubepithelial gastric tumors are common findings during upper gastrointestinal endoscopy. Tumor resection is mostly done laparoscopically, but there is still discussion concerning the size of lesion for which the treatment may be minimally invasive; additionally there is very little data available concerning patient outcome after minor access surgery.MethodsClinicopathologic features and survival data of 93 consecutive patients undergoing a combined laparoscopic‐endoscopic approach for gastric submucosal tumors were prospectively analyzed. Analysis included preoperative diagnostic work‐up, perioperative data, and postoperative complications. Follow‐up was carried out for patients with GIST to check for tumor recurrence.ResultsIt was possible to resect 88 of 93 lesions by the laparoscopic‐endoscopic approach, with tumor‐free margins in all patients. Intraoperative endoscopy facilitated exact tumor localization in 92 patients. Most lesions were removed by endoscopic‐laparoscopic wedge resection or, less frequently, by a combined transgastric approach. Mean operative time was 90.7 min; the postoperative hospitalization was 7.3 days. Adverse events appeared in 7.5%, and conversion to open surgery was required in 6.5%. For patients suffering from gastrointestinal stromal tumors, there was no tumor recurrence at a mean follow‐up of 40 months.ConclusionsCombined laparoscopic‐endoscopic “rendez‐vous” procedures are easy to perform and offer a curative approach for almost all gastric submucosal lesions. The technique is associated with low morbidity and short hospitalization. Though even patients with large GISTs of intermediate and high risk were treated, no tumor recurrence has been observed to date.
Specialist training in psychiatry in Europe – Results of the UEMS-survey
Authors: Lotz-Rambaldi Winfried , Schäfer Ines , ten Doesschate Roelof , Hohagen Fritz 
Published: March 2008

AbstractAccording to the aim of the Treaty of Rome from 1957 which postulated the free movement of workers throughout the European Union, the European Board of Psychiatry in the UEMS (European Union of Medical Specialists) carried out a comprehensive survey of training in psychiatry, including all member countries in order to evaluate the present state of training in psychiatry in each. The survey should indicate whether the training requirements [UEMS Section Psychiatry. Charter on training of medical specialists in the EU: requirements for the speciality psychiatry. European Archives of Psychiatry and Clinical Neuroscience 1997;247(Suppl.):S45–7; UEMS Section Psychiatry. Charter on training of medical specialists in the EU: requirements for the speciality psychiatry. <www.uemspsychiatry.org/board/reports/Chapter6-11.10.03.pdf>; 2003 [last revision]] have had an impact on the actual conditions of training in psychiatry in the member countries. We gathered 22 questionnaires from 31 national representatives involved and 424 questionnaires completed by the chief of training and the representative of trainees at the responding training centres from 22 countries. The results give an overview about the practice of training in psychiatry in many European countries. While there are great differences between the training centres in different countries, apparent progress towards developing high standards in training in psychiatry has been made.
COMMUTATIVITY DEGREES OF WREATH PRODUCTS OF FINITE ABELIAN GROUPS
Authors: EROVENKO IGOR V. , SURY B. 
Published: March 2008

AbstractWe compute commutativity degrees of wreath products $A \wr B$ of finite Abelian groups A and B. When B is fixed of order n the asymptotic commutativity degree of such wreath products is 1/n2. This answers a generalized version of a question posed by P. Lescot. As byproducts of our formula we compute the number of conjugacy classes in such wreath products, and obtain an interesting elementary number-theoretic result.
The relationship between total plasma carotenoids and risk factors for chronic disease among middle-aged and older men
Authors: Farwell Wildon R. , Michael Gaziano J. , Norkus Edward P. , Sesso Howard D. 
Published: March 2008

Individual plasma carotenoids have been associated with various chronic diseases but little is known about the relationship between total plasma carotenoids and risk factors for chronic diseases. In the Physicians' Health Study, we examined 492 men free of CVD and cancer for the relationship between total plasma carotenoids (the sum of α-carotene, β-carotene, lycopene, zeaxanthin, lutein and β-cryptoxanthin) and a wide variety of factors that predict chronic disease. Multivariate linear and logistic regression was performed to calculate parameter estimates (95 % CI) and OR (95 % CI) for total plasma carotenoids. In linear regression models, BMI, hypertension, alcohol intake and plasma levels of each lipid parameter and α-tocopherol significantly predicted levels of total plasma carotenoids. Upon adjustment for multiple chronic disease risk factors, the OR for levels of total plasma carotenoids greater than or equal to the median ( ≥ 1·301 μmol/l) was statistically significant for current smoking (OR 0·21; 95 % CI 0·06, 0·77), weekly alcohol ingestion (OR 2·30; 95 % CI 1·06, 4·99), daily alcohol ingestion (OR 2·46; 95 % CI 1·29, 4·67), each 100 mg/l increase in total cholesterol (OR 0·73; 95 % CI 0·58, 0·91), LDL-cholesterol (OR 1·48; 95 % CI 1·17, 1·89) and HDL-cholesterol (OR 1·58; 95 % CI 1·26, 1·99), each 100 mg/ml increase in intercellular adhesion molecule-1 (OR 0·70; 95 % CI 0·53, 0·93) and each 10 μmol/l increase in α-tocopherol (OR 1·33; 95 % CI 1·12, 1·57), using logistic regression. Few lifestyle and clinical risk factors appear to be related to levels of total plasma carotenoids; however, levels of biomarkers such as plasma lipids and α-tocopherol may be strongly related.
Cenozoic high Sr/Y volcanic rocks in the Qiangtang terrane, northern Tibet: geochemical and isotopic evidence for the origin of delaminated lower continental melts
Authors: LIU SHEN , HU RUI-ZHONG , FENG CAI-XIA , ZOU HAI-BO , LI CAI , CHI XIAO-GUO , PENG JIAN-TANG , ZHONG HONG , QI LIANG , QI YOU-QIANG , WANG TAO 
Published: March 2008

AbstractGeochemical and Sr–Nd–Pb isotopic data are presented for volcanic rocks from Zougouyouchaco (30.5 Ma) and Dogai Coring (39.7 Ma) of the southern and middle Qiangtang block in northern Tibet. The volcanic rocks are high-K calc-alkaline trachyandesites and dacites, with SiO2 contents ranging from 58.5 to 67.1 wt % The rocks are enriched in light REE (LREE) and contain high Sr (649 to 986 ppm) and relatively low Yb (0.8 to 1.2 ppm) and Y (9.5 to 16.6 ppm) contents, resulting in high La/Yb (29–58) and Sr/Y (43–92) ratios, as well as relatively high MgO contents and Mg no., similar to the compositions of adakites formed by slab melting in subduction zones. However, the adakitic rocks in the Qiangtang block are characterized by relatively low εNd(t) values (−3.8 to −5.0) and highly radiogenic Sr ((87Sr/86Sr)i=0.706–0.708), which are inconsistent with an origin by slab melting. The geochemistry and tectonics indicate that the adakitic volcanic rocks were most likely derived from partial melting of delaminated lower continental crust. As the pristine adakitic melts rose, they interacted with the surrounding mantle peridotite, elevating their MgO values and Mg numbers.
C1-stably shadowable chain components
Authors: SAKAI KAZUHIRO 
Published: March 2008

AbstractLetpbe a hyperbolic periodic saddle of a diffeomorphismfon a closed$C^\infty $manifoldM, and letCf(p) be the chain component offcontainingp. In this paper, we show that ifCf(p) isC1-stably shadowable, then (i) Cf(p) is the homoclinic class ofpand admits a dominated splitting$E \oplus F$(where the dimension ofEis equal to that of the stable eigenspace ofp); (ii) theCf(p)-germ offis expansive if and only ifCf(p) is hyperbolic; and (iii) whenMis a surface,Cf(p) is locally maximal if and only ifCf(p) is hyperbolic.