bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of polycomb group protein EZH2 in nevi and melanoma
Authors: McHugh Jonathan B. , Fullen Douglas R. , Ma Linglei , Kleer Celina G. , Su Lyndon D. 
Published: December 2006

Background:  Enhancer of zeste homolog 2 (EZH2), a polycomb group protein that regulates the cell cycle, has recently been implicated in the progression of several human cancers. We sought to determine the pattern of EZH2 expression in benign and malignant melanocytic tumors to see if EZH2 might play a role in melanoma pathogenesis and progression.Methods:  We identified and reviewed 11 compound nevi, 13 dysplastic nevi, 13 Spitz nevi, 9 in situ melanomas, 10 non‐metastatic invasive melanomas and 19 melanomas metastatic to lymph nodes from the University of Michigan pathology archives. Sections immunostained with anti‐EZH2 antibody were scored independently and blindly for staining intensity on a scale of 1–4 by three dermatopathologists. Results were analyzed and compared statistically.Results:  We observed an incremental increase in EZH2 expression from benign nevi to melanoma: scores of 1.18 and 1.08 for ordinary and dysplastic nevi, 1.7 and 1.78 for Spitz nevi and in situ melanoma, and 1.9 and 3.0 for invasive and metastatic melanoma, respectively. EZH2 expression for metastatic melanoma was significantly higher compared with invasive and in situ melanoma and benign nevi (p ≤ 0.01).Conclusions:  EZH2 protein levels increase incrementally from benign nevi to melanoma, which suggests that EZH2 may play a role in the pathogenesis and progression of melanoma.
Lipid Peroxidation and Glutathione Levels in Porcine Kidney PK15 Cells after Individual and Combined Treatment with Fumonisin B1, Beauvericin and Ochratoxin A
Authors: Klarić Maja Šegvić , Pepeljnjak Stjepan , Domijan Ana‐Marija , Petrik József 
Published: December 2006

Abstract:  Individual and combined effects of the mycotoxins fumonisin B1, beauvericin and ochratoxin A on cell viability, lipid peroxidation (TBARS) and intracellular glutathione (GSH) were studied on porcine kidney epithelial cells (PK15). Cells were treated with 0.05, 0.5 and 5 µg/ml of each mycotoxin or the combinations of two or all three applied in equal concentrations for 24 and 48 hr. Changes in cell viability, GSH and TBARS levels showed that the cytotoxic effects of these mycotoxins were concentration‐ and time‐dependent. After 24 hr, cell viability was significantly decreased by the exposure to 5 µg/ml of fumonisin B1 (25%), beauvericin (30%) and ochratoxin A (35%), as compared to controls. Only ochratoxin A (5 µg/ml) increased TBARS (56%), with further significant increase (85%) after 48 hr exposure. Fumonisin B1 and beauvericin significantly increased TBARS (57% and 80%, respectively) only when the highest dose was applied for 48 hr. After 24 hr, GSH was significantly decreased (18%) by ochratoxin A (0.05 µg/ml), whereas fumonisin B1 and beauvericin significantly decreased GSH at the concentration of 0.5 µg/ml. Combined treatment with fumonisin B1, beauvericin and ochratoxin A resulted mostly in additive effects especially after a 24‐hr exposure, although synergistic as well as antagonistic interactions could not be excluded depending on toxin concentrations and time of exposure. This is the first report on beauvericin‐induced effects on lipid peroxidation and GSH in animal cells.
Helping European Agriculture to Adjust — A Proactive Approach to Agricultural Policy
Authors: Blandford David , Hill Berkeley 
Published: December 2006

Helping European Agriculture to Adjust ‐ A Proactive Approach to Agricultural PolicyIn the European Union the next five to ten years are likely to witness continued pressures for agricultural policy reform. If cost‐effective ways of dealing with adjustment issues can be identified, reform may be less difficult to achieve. This article looks at the economic, equity and political arguments for providing adjustment assistance to farmers. Compensation for changes in policy has a role to play, but if poorly designed can inhibit structural change. A review of how farmers have reacted to policy reform in a number of OECD countries concludes that their ability to adapt is often under‐estimated, but that two characteristics are particularly important in facilitating change. The first is effectively functioning factor markets, particularly the market for land, and the second is the quality of human capital. These characteristics should be the primary focus of public policy to facilitate adjustment. The policies we propose would satisfy the Green Box requirements of the WTO that payments to farmers be minimally production and trade distorting. By establishing the conditions under which adapting to change is made easier, agricultural policy becomes sustainable, since adjustment is a process that is not limited to any single round of policy reform.
MINING TRUST VALUES FROM RECOMMENDATION ERRORS
Authors: O'DONOVAN JOHN , SMYTH BARRY 
Published: December 2006

Increasing availability of information has furthered the need for recommender systems across a variety of domains. These systems are designed to tailor each user's information space to suit their particular information needs. Collaborative filtering is a successful and popular technique for producing recommendations based on similarities in users' tastes and opinions. Our work focusses on these similarities and the fact that current techniques for defining which users contribute to recommendation are in need of improvement. In this paper we propose the use of trustworthiness as an improvement to this situation. In particular, we define and empirically test a technique for eliciting trust values for each producer of a recommendation based on that user's history of contributions to recommendations. We compute a recommendation range to present to a target user. This is done by leveraging under/overestimate errors in users' past contributions in the recommendation process. We present three different models to compute this range. Our evaluation shows how this trust-based technique can be easily incorporated into a standard collaborative filtering algorithm and we define a fair comparison in which our technique outperforms a benchmark algorithm in predictive accuracy. We aim to show that the presentation of absolute rating predictions to users is more likely to reduce user trust in the recommendation system than presentation of a range of rating predictions. To evaluate the trust benefits resulting from the transparency of our recommendation range techniques, we carry out user-satisfaction trials on BoozerChoozer, a pub recommendation system. Our user-satisfaction results show that the recommendation range techniques perform up to twice as well as the benchmark.
Methyl-3-indolylacetate inhibits cancer cell invasion by targeting the MEK1/2-ERK1/2 signaling pathway
Authors: Zhang Siyuan , Li Zhi , Wu Ximei , Huang Qing , Shen Han-Ming , Ong Choon-Nam 
Published: December 2006

Abstract
Epidemiologic studies have suggested an inverse correlation between dietary intake of cruciferous vegetables and cancer risk. It is thus of interest to investigate the anticancer potential of phytochemicals presented in cruciferous vegetables. In this study, methyl-3-indolylacetate (MIA), a cruciferous indole for which the bioactivity has not been previously reported, was found to significantly suppress the invasion of cancer cells stimulated by the 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Our data show that MIA pretreatments inhibited matrix metalloproteinase 9 (MMP-9) expression in a concentration-dependent manner, resulting in decreased MMP-9 activity. By using real-time reverse transcription-PCR, luciferase reporter gene assay, and electrophoretic mobility shift assay, we provided convincing evidence that MIA suppresses MMP-9 gene transcription via targeting the activator protein-1 signaling but not the nuclear factor-κB pathway. The TPA-induced mitogen-activated protein kinase (MAPK) activation cascade was also analyzed. Despite extensive activation of major MAPKs [c-Jun NH2-terminal kinase, p38, and extracellular signal-regulated kinase-1/2 (ERK1/2)] under TPA stimulation, only the ERK1/2 activation and its consequent nuclear translocation were found to be diminished by MIA. Interestingly, MIA did not affect the TPA-induced phosphorylation of either c-Raf or MAPK/ERK kinase-1/2 (MEK1/2), two upstream kinases of ERK. Moreover, using the in vitro kinase assay, MIA was shown to inhibit the kinase activity of MEK1/2, the upstream kinases of ERK, suggesting that MEK is the major molecular target of MIA. In conclusion, data from this study provided new insight into the anticancer potential of MIA, a cruciferous vegetable–derived indole compound. [Mol Cancer Ther 2006;5(12):3285–93]
Fanconi anemia D2 protein confers chemoresistance in response to the anticancer agent, irofulven
Authors: Wang Yutian , Wiltshire Timothy , Senft Jamie , Wenger Sharon L. , Reed Eddie , Wang Weixin 
Published: December 2006

AbstractThe Fanconi anemia-BRCA pathway of genes are frequently mutated or epigenetically repressed in human cancer. The proteins of this pathway play pivotal roles in DNA damage signaling and repair. Irofulven is one of a new class of anticancer agents that are analogues of mushroom-derived illudin toxins. Preclinical studies and clinical trials have shown that irofulven is effective against several tumor cell types. The exact nature of irofulven-induced DNA damage is not completely understood. Previously, we have shown that irofulven activates ATM and its targets, NBS1, SMC1, CHK2, and p53. In this study, we hypothesize that irofulven induces DNA double-strand breaks and FANCD2 may play an important role in modulating cellular responses and chemosensitivity in response to irofulven treatment. By using cells that are proficient or deficient for FANCD2, ATR, or ATM, we showed that irofulven induces FANCD2 monoubiquitination and nuclear foci formation. ATR is important in mediating irofulven-induced FANCD2 monoubiquitination. Furthermore, we showed that FANCD2 plays a critical role in maintaining chromosome integrity and modulating chemosensitivity in response to irofulven-induced DNA damage. Therefore, this study suggests that it might be clinically significant to target irofulven therapy to cancers defective for proteins of the Fanconi anemia-BRCA pathway. [Mol Cancer Ther 2006;5(12):3153–61]
Interleukin-13 receptor–targeted nanovesicles are a potential therapy for glioblastoma multiforme
Authors: Madhankumar A.B. , Slagle-Webb Becky , Mintz Akiva , Sheehan Jonas M. , Connor James R. 
Published: December 2006

Abstract
The difficulties associated with treatment of malignant brain tumors are well documented. For example, local infiltration of high-grade astrocytomas prevents the complete resection of all malignant cells. It is, therefore, critical to develop delivery systems for chemotherapeutic agents that ablate individual cancer cells without causing diffuse damage to surrounding brain tissue. Here, we describe sterically stable human interleukin-13 (IL-13)–conjugated liposomes, which efficiently bind to the brain cancer cells that overexpress the IL-13 receptor α2 protein. The conjugated liposomes bind to glioblastoma multiforme tissue specimens but not to normal cortex. Conjugating the liposomes with human IL-13 allows for specific binding to glioma cells and uptake of the liposomes via endocytosis. Delivering doxorubicin to glioma cells by IL-13–conjugated liposomes results in enhanced cytotoxicity and increased accumulation and retention of drug in the glioma cells compared with delivery of free drug. The therapeutic potential and targeting efficacy of the IL-13–conjugated liposomes carrying doxorubicin was tested in vivo using a s.c. glioma tumor mouse model. Animals receiving i.p. injections of IL-13–conjugated liposomes carrying doxorubicin for 7 weeks had a mean tumor volume of 37 mm3 compared with a mean volume of 192 mm3 in animals injected with nontargeted liposomes. These results strongly suggest that IL-13–conjugated liposomes carrying cytotoxic agents are a feasible approach for creating a nanovesicle drug delivery system for brain tumor therapy. [Mol Cancer Ther 2006;5(12):3162–9]
Biomechanical Evaluation of Different Fixation Methods for Tibial Eminence Fractures
Authors: Eggers Anne Kathleen , Becker Christoph , Weimann Andre , Herbort Mirco , Zantop Thore , Raschke Michael J. , Petersen Wolf 
Published: December 2006

Background Tibial eminence fractures have become more common, but the best method of fixing these fractures arthroscopically has not been elucidated. Objective The objective of this study is to assess the stability of different fixation methods for tibial eminence fractures. Study Design Controlled laboratory study. Methods The authors evaluated the initial fixation strength of 4 different fixation techniques for tibial eminence fractures (2 screws, 1 screw, suture fixation with 1 mm Ethibond, and suture fixation with No. 5 FiberWire) using single cycle and cyclic loading tests. The tests were performed by loading the knee with an anterior tibial shear force. Results Suture cerclage with the FiberWire showed significantly higher maximum load (599.6 N) and stiffness (36.99 N/mm) than all other fixation methods tested. The lowest maximum load in the single cycle loading test was observed in the group with 2 screws (1 screw, 371.2 N; 2 screws, 249.8 N). The lowest stiffness was found in the Ethibond group (14.5 N/mm). After 1000 cycles of loading, the maximum load of the FiberWire fixation was significantly higher than the maximum load of the Ethibond fixation (399.4 N), the fixation with 1 screw (354.2 N), and the fixation with 2 screws (301.5 N). Conclusion These biomechanical data suggest that under cyclic loading conditions, suture fixation of tibial eminence fractures provides more fixation strength than screw fixation. Clinical Relevance A second screw has no positive effect on the biomechanical characteristics of screw fixation.
Stroke Affects the Coordination of Gaze and Posture During Preplanned Turns While Walking
Authors: Lamontagne Anouk , Paquette Caroline , Fung Joyce 
Published: December 2006

Background In healthy subjects, the act of walking and turning is accomplished by a sequential horizontal reorientation of gaze, head, and body toward the direction of the turn. Subjects with stroke, however, have difficulty altering their walking direction and present with loss of balance when performing a head turn or whole body rotation. Objective. To study, in a pilot case study, the spatial and temporal coordination of gaze and posture during preplanned turns executed while walking in severely disabled and mildly disabled subjects with stroke as compared to a healthy control walking at slow speed. Methods. Horizontal plane orientations of gaze, head, thorax, pelvis, and feet as well as the body’s center of mass (CoM) trajectory were analyzed as subjects were walking straight or executing a 90-deg turn. Results. Subjects with stroke revealed altered orientation and sequencing of gaze body segments. These alterations were more pronounced in the most severely disabled subject with stroke, especially when turning to the nonparetic side as compared to the paretic side. Conclusions. These findings suggest an altered coordination of gaze and posture during steering of locomotion in subjects with stroke. This altered coordination is likely due to a complex interaction of motor, sensory, and biomechanical factors that may explain the poor balance and poor control of heading direction during walking and turning in stroke.
Étude par spectroscopie infrarouge de l'eau adsorbée par la saponite-calcium
Authors: Suquet H. , Prost R. , Pezerat H. 
Published: December 2006

RésuméLes hydrates à deux couches et à une couche d'une saponite calcique ont été étudiés par infrarouge. Les résultats expérimentaux indiquent que dans l'hydrate à deux couches les molécules d'eau directement coordinées à l'ion calcium sont liées par liaisons hydrogène fortes d'une part aux atomes d'oxygène de surface chargés négativement et d'autre part à d'autres molécules d'eau. En revanche, dans l'hydrate à une couche où chaque ion calcium est solvaté par un minimum de 2 molécules d'eau, il n'y a pas de liaison hydrogène intermoléculaire. La plupart des molécules d'eau donnent une seule liaison hydrogène forte. Chacune des molécules d'eau forme un pont hydrogène, l'une avec le feuillet supérieur et l'autre avec le feuillet inférieur.
Attribution of the OH stretching bands of kaolinite
Authors: Rouxhet P. G. , Samudacheata Ngo , Jacobs H. , Anton O. 
Published: December 2006

AbstractExamination of the infrared spectra of synthetic kaolinites, with various degrees of substitution of hydroxyls by OD groups, allows a more detailed attribution of the stretching bands due to inner-surface hydroxyls of kaolinite. Among the three innersurface OH groups of the unit cell, two are nearly perpendicular to the sheet and give coupled vibrations which are responsible for the bands around 3695 and 3670 cm−1; the band near 3655 cm−1 is due to the other hydroxyl, which is lying close to the sheet.
Etude par microscope electronique des differents etats d'hydration de la sepiolite
Authors: Rautureau M. , Mifsud A. 
Published: December 2006

RésuméLa sépiolite chauffée à 300°C et 480°C sous vide a été étudiée avec un microscope électronique en haute-résolution. Les clichés donnent une photographie réelle des structures de la sépiolite naturelle et de la sépiolite anhydre, telles qu'elles ont été calculées par la cristallographie. Les résultats obtenus sont en accord avec ceux de la diffraction des rayons X et de la spectroscopie infrarouge.
New data on ktenasite
Authors: Raade G. , Elliott C. J. , Fejer E. E. 
Published: December 2006

SummaryKtenasite from Glomsrudkollen zinc mine, Modum, Norway, is menoclinic, space group P21/c. The cell dimensions are a 5·598, b 6·121, c 23·762Å, β 95·55° The chemical formula is (Cu3·5Zn1·5)(SO4)2 (OH)6·6H2O with Z = 2; ρcale 2·96 g/cm3, ρobs 2·94 g/cm3. The mineral is biaxial negative with α (colourless) 1·574, β (bluish green) 1·615, γ (light green) 1·628, 2Vα 59°.
Forsterite chondrites; the meteorites Kakangari, Mount Morris (Wisconsin), Pontlyfni, and Winona
Authors: Graham A. L. , Easton A. J. , Hutchison R. 
Published: December 2006

SummaryPontlyfni and Mount Morris (Wisconsin) are briefly described. Chondrule structure is absent from both, the latter is coarsely crystalline and both are sulphide-rich and contain forsterite and enstatite. Kakangari has well-defined chondrules; its silicate and sulphide minerals are unequilibrated, but similar to those of Pont-lyfni and Mount Morris (Wisconsin). New bulk chemical analyses are presented; Pontlyfni has 33·84 % total Fe and 7·04 % S; Mount Morris (Wisconsin) has 19·88 % total Fe and 4·72 % S; and Kakangari has 22·79 % total Fe and 5·30 % S. These three meteorites, together with Winona, have ordinary chondritic Mg/Si ratios, which result in their having abundant forsterite; this distinguishes them from E-chondrites. The four meteorites have Mg/Si ratios lower than those of C-chondrites, and they are more reduced than C or ordinary chondrites. These four unusual stones, therefore, have some chemical similarities, for example all have Mg/Si (atomic) of about 0·95, mean olivine composition ranging from Fa1 to Fa5, and significant Cr in the sulphide. Cumberland Fails chondritic xenoliths and other exotic fragments in polymict meteorites may be related to the four stones, which may ultimately prove to belong to a distinct chemical group of chondrites related to the irons of Group IAB.These meteorites are described and discussed together because they have similarities in mineralogy, bulk composition, and oxidation state. All have more than 10 % forsterite (i.e. an olivine in the range Fa0·10). In our study we investigated the mineral chemistry of, first, Kakangari (Graham and Hutchison, 1974), and then Mount Morris (Wisconsin), with cursory examination of the previously described Winona (Mason and Jarosewich, 1967) and Cumberland Falls chondritic fraction (Binns, 1969; Jarosewich, 1967). By chance, the British Museum (Natural History) acquired the Pontlyfni stone during the course of this work. Pontlyfni fell in Wales in 1931, but is so far undescribed; it proved to be chemically and mineralogically similar to Kakangari. We thank Mr. J. R. Owen, the original owner, for its timely addition to the Museum's Collection.
Geochemical relationships between some pallasites and iron meteorites
Authors: Scott Edward R. D. 
Published: December 2006

SummaryPublished analyses for 14 trace and minor siderophilic elements have been interpreted to indicate a close relationship between the IIIAB iron meteorites and the main group of pallasites. These two groups respectively account for about 32 and 75 % of all irons and pallasites. The composition of the metal in the main group pallasites is very close to that calculated for the liquid after 80 % of a IIIAB iron melt has fractionally crystallized. The pallasites probably formed by the intrusion of residual molten metal from the core into a surrounding olivine mantle.
Transcription profile of a human breast cancer cell line expressing MMTV-like sequences
Authors: Fernandez-Cobo Mariana , Melana Stella M , Holland James F , Pogo Beatriz GT 
Published: December 2006

Abstract

Background
It has been postulated that inflammation caused by certain viruses might result in cancer. Recently, it was shown that childhood lymphoblastic leukemia, breast and ovarian cancers express an interferon-related signature, providing support for this notion. We have previously shown that 38% of the sporadic breast cancers contain MMTV-like env gene sequences. To find out if the presence and expression of MMTV-like sequences correlated with an inflammatory phenotype, we have compared the expression profile of two sublines of MCF-7 cells, one containing the MMTV-like sequences (env+), the other one lacking them (env-).

Results
The results indicated that there were 47 differentially expressed genes between the two sublines. Among 27 upregulated genes in the env+ cells there were 7 interferon-related genes, 5 TNF-connected genes and 2 TGFβ-related genes.

Conclusion
These results suggest that the env+ cells were most likely responding to an infectious agent, and support the hypothesis that a viral infection may play a role in breast cancer pathogenesis.