bluuebunny/crossref_metadata_2025_split huggingface dataset:
A potential link between inflammatory profiles, clinical pain, pain catastrophizing and long‐term outcomes after total knee arthroplasty surgery
Authors: Giordano Rocco , Capriotti Camilla , Gerra Maria Carla , Kappel Andreas , Østgaard Svend Erik , Dallabona Cristina , Arendt‐Nielsen Lars , Petersen Kristian Kjær‐Staal 
Published: February 2024

AbstractBackgroundChronic postoperative pain after total knee replacement (TKR) is a major clinical problem. It is still unclear if specific inflammatory mediators are associated with long‐term postoperative pain complications. The current exploratory study aimed to (1) evaluate a multiplex of inflammatory mediators 5 years after TKR surgery in patients with different degrees of postoperative pain intensities and (2) study any association of the markers with clinical pain intensity, cognitive and functional outcomes.MethodsPlasma samples were collected 5 years after TKR surgery from 76 knee patients (43 females; 33 males) and analysed for 44 inflammatory markers. Pain (using visual analogue scale, VAS), the pain catastrophizing scale (PCS) and the Oxford knee score (OKS) were evaluated. Patients were categorized as high or low groups based on VAS, PCS and OKS scores. Associations between inflammatory markers, VAS, PCS and OKS were analysed and the marker expressions were compared between groups.ResultsPearson's correlations found 12 biomarkers associated with VAS (p < 0.05), 4 biomarkers with PCS and 3 biomarkers with OKS (p < 0.05). Four markers were altered in patients suffering from high compared to low chronic postoperative pain, three markers were altered in high compared to low catastrophizers and three markers were altered in patients with poor functional scores (p < 0.05).ConclusionsThe present exploratory study suggests that low‐grade inflammation might be present in a subset of patients with high pain, high catastrophizing and low function 5 years after TKR. These exploratory results provide insights into some of the long‐term postoperative complications after TKR surgery.Significance StatementThis exploratory study evaluated a subset of inflammatory markers and the association to clinical pain intensity, knee function and pain catastrophizing in patients 5 years after total knee replacement surgery. Our results provide insights into the understanding of the underlying mechanisms that may drive the long experience of pain after TKR surgery.
Design and evaluation of collaborative decision‐making application for patient care in the emergency department
Authors: Shams‐Vahdati Neda , Shams Vahdati Samad , Samad‐Soltani Taha 
Published: February 2024

AbstractBackground and AimsCollaboration has become a crucial element of effective healthcare delivery in the emergency department (ED). In high‐pressure environments, healthcare providers can prioritize patients by consulting with other specialists to gain diverse perspectives and arrive at a shared understanding of the best course of action. It was conducted for the purpose of designing and evaluating the collaborative decision‐making application for patient care in the ED.MethodsThe present applied research study was conducted between April 1, 2021 and May 31, 2023 at Imam Reza Hospital of Tabriz University of Medical Sciences. The study was conducted in three phases: exploration, development, and evaluation, utilizing modern technologies such as Flutter and Node.js to design and program the application. The effectiveness of the system was evaluated using established measures, including the think‐aloud protocol, user experience questionnaire, and Likert‐scale questionnaires developed by Ghadri et al.ResultsThe average scale for attractiveness was 2.03, perspicuity was 2.90, efficiency was 2.40, dependability was 1.93, stimulation was 2.48, and novelty was 2.78. Additionally, 71% of physicians gave a very good rating to the accessibility of necessary information at any time, motivation to use the system for accessing information, usefulness of the system compared to the time spent using it throughout the day. Furthermore, 57% of physicians gave a very positive rating to sharing information and knowledge, ease of using the search function and accessing the system, user control and monitoring, free access to the system, and support from colleagues and system users.ConclusionThe study suggests that introducing Information and Communication Technology such as medical apps can improve healthcare delivery by streamlining patient care, promoting effective teamwork, and reducing medical errors and treatment delays.
Lethal disseminated intravascular coagulation induced by primary and metastatic neuroendocrine prostate cancer
Authors: Ando Takashi , Sasaki Taro , Naito Makoto 
Published: February 2024

IntroductionNeuroendocrine prostate cancer has a poor prognosis. Although disseminated intravascular coagulation associated with malignancy can be lethal, it very rarely occurs among patients with primary neuroendocrine prostate cancer.Case presentationAn 80‐year‐old man presented to our hospital with bloody sputum. Blood examination indicated disseminated intravascular coagulation. Serum levels of prostate‐specific antigen and neuron‐specific enolase were 44.274 and 176 ng/mL, respectively. Core needle biopsies of an irregular mass in the prostate and a metastatic tumor in the left iliac bone showed similar neuroendocrine carcinoma cells. Hence, the patient was diagnosed with disseminated intravascular coagulation associated with primary and metastatic neuroendocrine prostate cancer. Unfortunately, he passed away 3 weeks after the biopsies.ConclusionGiven the difficulty of effectively treating metastatic neuroendocrine prostate cancer among patients in poor physical condition due to disease progression, identifying a new well‐tolerated treatment modality is imperative.
Black men's perceptions of mothers as primary support for wellness
Authors: Summers LaTonya M. , Robinson Michael , Montague Karlesia 
Published: February 2024

AbstractBlack men regard their mothers as the source of primary support and may benefit from having counselors incorporate their mothers into the counseling process. In this study, the researchers attempted to understand Black men's lived experiences and the impact of their mother's influence upon their wellbeing. Drawing on interviews with 10 Black men, the researchers excavated 5 themes related to development and wellness of Black men as clients. The researchers discussed the results with implications for counselors, along with current study's limitations.
JAM2 is a prognostic biomarker and inhibits proliferation, metastasis and epithelial–mesenchymal transition in lung adenocarcinoma
Authors: Dong Yanxin , Zhang Jiale , Xie Shun , Di Shouyin , Fan Boshi , Gong Taiqian 
Published: February 2024

AbstractBackgroundJunctional adhesion molecule 2 (JAM2) plays a pivotal role in various biological processes, including proliferation, metastasis and angiogenesis, contributing to tumor progression. While previous studies have highlighted the polarizing functions of JAM2 in different cancer types, its specific role in lung adenocarcinoma (LUAD) remains unclear.MethodsIn this study, we harnessed multiple public databases to analyze the expression and prognostic significance of JAM2 in LUAD. Using the Linkedomics database, Matescape database and R package, we explored the associated genes, the potential biological functions and the impact of JAM2 on the tumor microenvironment. Our findings from public databases were further validated using real‐time quantitative PCR, western blot and immunohistochemistry. Additionally, in vitro experiments were conducted to assess the influence of JAM2 on LUAD cell proliferation, invasion, migration, apoptosis and epithelial–mesenchymal transition. Furthermore, we established a xenograft model to investigate the in vivo effects of JAM2 on tumorigenesis.ResultsOur results revealed a significant downregulation of JAM2 in LUAD, and patients with low JAM2 expression exhibited unfavorable overall survival outcomes. Functional enrichment analysis indicated that JAM2 may be associated with processes such as cell adhesion, extracellular matrix, cell junctions and regulation of proliferation. Notably, increased JAM2 expression correlated with higher tumor microenvironment scores and reduced immune cell abundance. Furthermore, overexpression of JAM2 induced apoptosis, suppressed tumor proliferation and exhibited potential inhibitory effects on tumor invasion and migration through the modulation of epithelial–mesenchymal transition. Additionally, in vivo experiments confirmed that JAM2 overexpression led to a reduction in tumor growth.ConclusionOverall, our study highlights the clinical significance of low JAM2 expression as a predictor of poor prognosis in LUAD patients. Moreover, JAM2 was found to exert inhibitory effects on various aspects of tumor progression. Consequently, JAM2 emerges as a promising prognostic biomarker and a potential therapeutic target for LUAD patients.
Regioselective synthesis of (Z)‐5‐methylene‐containing morpholin‐2‐ones
Authors: Camargo Adriano F. , Pereira Genilson S. , Mittersteiner Mateus , Marangoni Mário A. , Moraes Paulo A. , Bonacorso Helio G. , Martins Marcos A. P. , Zanatta Nilo 
Published: February 2024

AbstractAn efficient, one‐pot, protecting‐group‐free, and regioselective protocol to obtain enantiomerically pure (Z)‐5‐methylene‐containing morpholin‐2‐ones, using α‐amino acids and 5‐bromo‐4‐methoxy enones as selective starting materials, is reported. The reaction was fully selective in providing the (Z)‐regioisomer in all cases, regardless of the α‐amino acid used; however, when a secondary amino group was used, only the (E)‐isomer was observed. The synthesized compounds were obtained at yields of up to 92%, and their structure was unambiguously assigned by x‐ray and 2D‐NMR experiments.
Comparative costs and potential affordability of a multifaceted intervention to improve treatment outcomes among people with HIV who inject drugs in Russia: economic evaluation of the LINC‐II randomized controlled trial
Authors: Rosen Sydney , Blokhina Elena , Truong Ve , Bereznicka Agata , Gnatienko Natalia , Quinn Emily , Lioznov Dmitry , Krupitsky Evgeny , Michals Amy , Lunze Karsten , Samet Jeffrey H. 
Published: February 2024

AbstractIntroductionThe LINC‐II randomized controlled trial in St. Petersburg, Russia for HIV‐positive adults who inject drugs found that a multi‐component intervention including initiation of antiretroviral therapy (ART) during admission to an addiction hospital, strengths‐based case management and naltrexone significantly increased 12‐month HIV viral suppression and ART retention. We conducted a comparative cost analysis to determine if the 12‐month cost of the intervention is affordable within the current Russian health system.MethodsWe used LINC‐II trial records and questionnaire responses to calculate the resources utilized by each participant in the study, including inpatient days, medications, laboratory tests, outpatient consultations, case manager interactions and opioid medication treatment. Quantities of resources utilized were multiplied by unit costs for each resource estimated from the service fee or price lists used by the study facilities for each specific service delivered. We report the average cost/study primary (viral suppression at 12 months) or secondary (retention in care at 12 months) outcome/participant in 2021 USD and compare costs between study arms.ResultsThe trial enrolled 225 participants (111 intervention, 114 control) between September 2018 and December 2020. Viral suppression, non‐suppression and missing suppression results were 28% and 14%, 49% and 37%, and 31% and 41% for the control and intervention arms, respectively. Retention results were 35% and 51% for the control and intervention arms, respectively. The average cost per study participant was $2714 in the control arm and $4342 in the intervention arm. The average cost per participant virally suppressed at 12 months was $3662 (control) and $6355 (intervention). The average cost per participant retained at 12 months was $4050 (control) and $5448 (intervention). For those retained, the cost difference between the arms was comprised of opioid treatment (35%), case management (31%), outpatient visits (18%) and additional days of ART (12%).ConclusionsThe LINC‐II intervention increased the cost of care for HIV‐positive people who inject drugs in Russia significantly, but some components of the intervention, particularly earlier initiation of ART and case management, may be justifiable due to their success in reaching a challenging subgroup of the population in need.Clinical Trial NumberNCT03290391
The hidden costs of austerity for social programmes in the Global South: Qualitative insights from two complementary services in Ghana's Livelihood Empowerment Against Poverty Programme
Authors: Mohammed Abdul‐Rahim 
Published: February 2024

AbstractPromoted by the International Monetary Fund (IMF) in particular, fiscal austerity measures have been extensively implemented across the world in the last decade as a policy prescription to address the impacts of economic shocks. Consequently, the implementation of austerity has engendered a vast body of austerity impact assessment literature in the Global North, in particular. Although austerity measures have been equally implemented (and longer) in Global South contexts, the same level of intellectual curiosity has not been dedicated to understanding the human and social costs of austerity in the Global South. To help fill this gap, this paper examines the implications of fiscal austerity for the delivery of two complementary programmes linked to Ghana's conditional cash transfer (CCT) — the Livelihood Empowerment Against Poverty (LEAP) programme. The paper adopts a qualitative design where semi‐structured interviews are conducted with (a) two LEAP officers, (b) two headteachers and (c) 35 LEAP beneficiaries in northern Ghana. The findings show that within the current context of fiscal austerity, mainly mediated through the government's commitment to “cut expenditures to the bone”, the two complementary services have been unfunded and thus do not exist in practice. The non‐existence of complementary services has dire consequences for children's education as well as undermines Ghana's poverty reduction attempts through the implementation of the LEAP programme.
Simultaneous determination of selected ionophoric coccidiostats and amino acids in feed premixes using high‐performance liquid chromatography‐ultraviolet detection method
Authors: Zayed Sahar , Belal Fathalla , Barghash Sona , Fouad Fatma 
Published: February 2024

The combination of ionophoric coccidiostats and amino acids (AAs) is important in poultry feeding to enhance immunity and improve the growth and feed efficiency of birds suffering from coccidiosis. A simple, rapid, and economical high‐performance liquid chromatography‐ultraviolet detection (HPLC‐UV) method for the simultaneous determination of three ionophoric coccidiostats, namely salinomycin (SAL), maduramicin (MAD), and monensin (MON) in addition to three AAs; L‐tryptophan (L‐TRP), alpha‐ketoleucin (KLEU), and L‐valine (L‐VAL) in feed premixes was developed and validated. Chromatographic separation was achieved in less than 12 min using a phenyl hexyl column with a mobile phase consisting of acetonitrile/methanol/water (25:20:55, v/v/v) adjusted to pH 3 using phosphoric acid. Isocratic elution was performed at a flow rate of 1 mL/min with UV detection at 210 nm. The method showed good linearity in the ranges 0.50–5.0 mg/mL for MON, 0.20–2.0 mg/mL for MAD and SAL, 10.0–100.0 μg/mL for L‐TRP and KLEU, and 50.0–500.0 μg/mL for VAL. The developed method was successfully applied to determine the studied analytes in feed premixes with good recoveries and precision. The good validation criteria of the proposed method allow its utilization in quality control laboratories.
Surface molecularly imprinted polymers based on magnetic multi‐walled carbon nanotubes for the highly selective purification of resveratrol from crude extracts of Vitis vinifera, Arachis hypogaea, and Polygonum cuspidatum
Authors: Cao Run‐Ze , Gai Qing‐Yan , Jiao Jiao , Wang Yuan , Fu Yu‐Jie , Nie Si‐Ming , Lu Yao 
Published: February 2024

In this work, surface molecularly imprinted polymers based on magnetic multi‐walled carbon nanotubes were prepared for the specific recognition and adsorption of resveratrol. The functionalization of magnetic multi‐walled carbon nanotubes and the synthesis process of surface molecularly imprinted polymers were optimized. Characterizations were performed to demonstrate the successful synthesis of the imprinted materials. The imprinted materials showed satisfactory adsorption capacity of resveratrol (45.73 ± 1.72 mg/g) and excellent selectivity (imprinting factor 2.89 ± 0.15). In addition, the imprinted materials were used as adsorbents in molecularly imprinted solid‐phase extraction for the purification of resveratrol from crude extracts of some food and medicinal resources, achieving recoveries of 93.69%−95.53% with high purities of 88.37%−92.33%. Moreover, the purified products exhibited extremely strong free radical scavenging activity compared with crude extracts. Overall, this work provided a promising approach for the highly selective purification of resveratrol from natural resources, which would contribute to the application of this valuable compound in the food/nutraceutical fields.
Indigenous knowledge and species assessment for the Alexander Archipelago wolf: successes, challenges, and lessons learned
Authors: Brooks Jeffrey J. , Markegard Sarah I. , Langdon Stephen J. , Anderstrom Devlin Shaag̱aw Éesh , Douville Michael Gitwaayne , George Thomas A. , Jackson Michael Kauish , Jackson Scott Gus'tú , Mills Thomas Ḵaachkutí , Ramos Judith Dax̠ootsú , Rowan Jon Yaanasgít , Sanderson Tony , Smythe Chuck 
Published: February 2024

AbstractThe United States Fish and Wildlife Service in Alaska, USA, conducted a species status assessment for a petition to list the Alexander Archipelago wolf (Canis lupus ligoni) under the Endangered Species Act in 2020‐2022. This federal undertaking could not be adequately prepared without including the knowledge of Indigenous People who have a deep cultural connection with the subspecies. Our objective is to communicate the authoritative expertise and voice of the Indigenous People who partnered on the project by demonstrating how their knowledge contributed to the species status assessment. The Indigenous knowledge applied in the assessment is the cultural and intellectual property of those who have shared it. We employed rapid appraisal research to expeditiously develop a preliminary and qualitative understanding of Indigenous People's cultural and ecological knowledge of Alexander Archipelago wolves. We used semi‐directed interviewing and inductive coding from grounded theory for text analysis. Indigenous knowledge contributed to the agency's understanding of the Alexander Archipelago wolf in Southeast Alaska and helped the agency with their classification decision. Indigenous research partners explained the rich cultural significance and position of wolves in Tlingit society and described human–wolf relationships and ecological interactions. The agency used a single‐species assessment approach based in species ecology and conservation biology, whereas the Indigenous wolf experts applied a multi‐species, community ecology approach based in a sociocultural context of balance and respect. The Indigenous wolf experts successfully addressed knowledge gaps identified by the agency. The partners were challenged by a short regulatory timeframe that did not allow for comprehensive study of Indigenous knowledge and constrained review and feedback by Indigenous experts. The United States Fish and Wildlife Service learned that its assessment framework was not designed to account for an Indigenous worldview. To level the playing field, the agency and Indigenous experts should discuss how to co‐develop an assessment framework that equitably applies both perspectives.
Salinity as a key factor affecting viral activity and life strategies in alpine lakes
Authors: Zang Lin , Liu Yongqin , Jiao Nianzhi , Zhong Kevin Xu , Song Xuanying , Yang Yunlan , Cai Lanlan , Liu Keshao , Mao Guannan , Ji Mukan , Zhang Rui 
Published: February 2024

AbstractViruses are major players in the biosphere, yet little is known about their dynamics and life strategies in alpine lakes, particularly those on the Tibetan Plateau. We investigated microbial abundance, viral dynamics, and viral life strategies in 10 high‐altitude Tibetan lakes and found that they harbor high levels of active viruses. Salinity was identified as a crucial factor influencing viral abundance, dynamics, as well as viral life strategies. Lytic and lysogenic viral productions in moderate‐ and high‐salinity lakes were significantly higher than those in freshwater lakes. A trade‐off between viral life strategies resulted in a switch from lysis to lysogeny in high‐salinity lakes. Virus‐mediated cell lysis and virus decay in sampled Tibetan lakes could release about 162.72 and 2.84 μg C L−1 d−1, respectively. These findings revealed the crucial role of viruses in the carbon cycle of Tibetan lakes, and a switch in viral life strategies may impact their contribution to the carbon cycle. The potential impact of salinity changes triggered by climate change on the carbon cycle in alpine lakes worldwide is highlighted.
Powders to Thin Films: Advances in Conjugated Microporous Polymer Chemical Sensors
Authors: Rajput Saurabh Kumar , Mothika Venkata Suresh 
Published: February 2024

AbstractChemical sensing of harmful species released either from natural or anthropogenic activities is critical to ensuring human safety and health. Over the last decade, conjugated microporous polymers (CMPs) have been proven to be potential sensor materials with the possibility of realizing sensing devices for practical applications. CMPs found to be unique among other porous materials such as metal–organic frameworks (MOFs) and covalent organic frameworks (COFs) due to their high chemical/thermal stability, high surface area, microporosity, efficient host–guest interactions with the analyte, efficient exciton migration along the π‐conjugated chains, and tailorable structure to target specific analytes. Several CMP‐based optical, electrochemical, colorimetric, and ratiometric sensors with excellent selectivity and sensing performance were reported. This review comprehensively discusses the advances in CMP chemical sensors (powders and thin films) in the detection of nitroaromatic explosives, chemical warfare agents, anions, metal ions, biomolecules, iodine, and volatile organic compounds (VOCs), with simultaneous delineation of design strategy principles guiding the selectivity and sensitivity of CMP. Preceding this, various photophysical mechanisms responsible for chemical sensing are discussed in detail for convenience. Finally, future challenges to be addressed in the field of CMP chemical sensors are discussed.