bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study protocol for a prospective, randomized, multicenter trial to investigate the influence of peripheral nerve stimulation on patients with chronic sacroiliac joint syndrome (SILENCING)
Authors: Sargut Tarik Alp , Tkatschenko Dimitri , Früh Anton , Tüttenberg Jochen , Heckert Alexander , Fleck Steffen , Kuckuck Anja , Bayerl Simon Heinrich 
Published: March 2024

Abstract
Background
The prevalence of sacroiliac joint pain (SIJP) is estimated to be 10–30% in patients with chronic low back pain. Numerous conservative and surgical treatment modalities for SIJP have been described with limited evidence regarding long-term pain relief.
Spinal cord stimulation (SCS) is a well-established technique to treat patients with chronic low back pain. However, the effect on patients with SIJP is not consistent. Therefore, peripheral nerve stimulation (PNS) for chronic SIJP was implemented in experimental trials. Clinical data on PNS for SIJP is still lacking. The authors present a case series and a protocol for a prospective, multicenter study to determine the effect of PNS in patients with chronic intractable SIJP.

Method
A multicenter, prospective randomized controlled trial was designed. Patients with chronic intractable SIJP will be recruited and randomized in a 4:3 ratio to either the peripheral nerve stimulation group or to the best medical treatment group. A total of 90 patients are planned to be enrolled (52 in the PNS group and 38 in the BMT group). Patients in the intervention group receive a percutaneous implantation of a unilateral or bilateral lead which is externalized for a trial phase for 3–14 days. After trial phase only patients with at least 50% reduction of pain receive an impulse generator for permanent stimulation. Regular visits for participants are planned on day 0, after 3 months (± 30 days), 6 months (± 30 days), and 12 months (± 60 days). The primary outcome measurements is the difference in Numeric Pain Rating Scale (NRS) between baseline and after 6 months. Secondary outcomes is improvement of pain associated disability (ODI) and improvement of health-related quality of life after 6 and 12 months.

Discussion
We have described the protocol for a prospective, multicenter, randomized trial evaluating the influence of PNS on patients with chronic sacroiliac joint syndrome. We believe that PNS on patients with chronic sacroiliac joint syndrome will show promising results regarding pain relief and quality of life in comparison to BMT after 12 months. The design of this trial promises high evidence in comparison to the data to date.

Trial registration
ClinicalTrials.gov, NCT05357300. Registered on April 26, 2022.
Resting heart rate associations with violence exposure and posttraumatic stress symptoms: sex differences in children
Authors: Wiltshire Charis N. , Kouri Nicole , Wanna Cassandra P. , Minton Sean T. , France John M. , Reda Mariam H. , Davie William , Basarkod Sattvik , Winters Sterling , Hinrichs Rebecca , Stenson Anais F. , Jovanovic Tanja 
Published: March 2024

Abstract
Background
Traumatic events experienced in childhood can lead to increased risk of cardiovascular disorders in adulthood. Black Americans are disproportionately affected, as they are at increased risk for experiencing childhood trauma and cardiovascular diseases in adulthood. One of the hypothesized mechanisms of this association is through long-lasting dysregulation of the autonomic nervous system, a hallmark physiological biomarker of posttraumatic stress disorder (PTSD), which is twice as prevalent in women compared to men.

Methods
Ninety-one, majority Black American children, aged 9 were recruited to be a part of our longitudinal study of child development at research centers in Atlanta, GA and Detroit, MI. Resting HR was measured through a electrocardiogram (ECG) recording using the Biopac MP150. Self-report measures of violence exposure and PTSD symptoms were administered by research staff.

Results
Children with more violence exposure reported increased PTSS as well as lower resting HR. Regression analysis showed evidence of sex modifying this relationship, (B = -0.64, p < 0.05), such that the association between resting HR and PTSS was stronger in girls than in boys. In our exploratory analysis with standard clinical cutoffs of resting HR, the normative HR group was found to significantly moderate the relationship between violence exposure and PTSS in boys, (B = -2.14, p < 0.01), but not girls (B = -0.94, p = 0.27).

Conclusion
In our sample of primarily Black urban children, we found that violence exposure was associated with slower, more adult-like HR, that girls showed greater PTSS associated with slower HR while boys did not, and that girls with lower than normative HR showed significantly higher PTSS compared to girls with normative HR. Our sample’s demonstration of psychological consequences in addition to the physiological implications could provide new information about a psychobiological sequelae of violence exposure.
The spontaneously produced lysogenic prophage phi456 promotes bacterial resistance to adverse environments and enhances the colonization ability of avian pathogenic Escherichia coli strain DE456
Authors: Li Dezhi , Liang Wei , Huang Zhiqiang , Ma Wenwen , Liu Qing 
Published: March 2024

AbstractIn the last decade, prophages that possess the ability of lysogenic transformation have become increasingly significant. Their transfer and subsequent activity in the host have a significant impact on the evolution of bacteria. Here, we investigate the role of prophage phi456 with high spontaneous induction in the bacterial genome of Avian pathogenic Escherichia coli (APEC) DE456. The phage particles, phi456, that were released from DE456 were isolated, purified, and sequenced. Additionally, phage particles were no longer observed either during normal growth or induced by nalidixic acid in DE456Δphi456. This indicated that the released phage particles from DE456 were only phi456. We demonstrated that phi456 contributed to biofilm formation through spontaneous induction of the accompanying increase in the eDNA content. The survival ability of DE456Δphi456 was decreased in avian macrophage HD11 under oxidative stress and acidic conditions. This is likely due to a decrease in the transcription levels of three crucial genes—rpoS, katE, and oxyR—which are needed to help the bacteria adapt to and survive in adverse environments. It has been observed through animal experiments that the presence of phi456 in the DE456 genome enhances colonization ability in vivo. Additionally, the number of type I fimbriae in DE456Δphi456 was observed to be reduced under transmission electron microscopy when compared to the wild-type strain. The qRT-PCR results indicated that the expression levels of the subunit of I fimbriae (fimA) and its apical adhesin (fimH) were significantly lower in DE456Δphi456. Therefore, it can be concluded that phi456 plays a crucial role in helping bacterial hosts survive in unfavorable conditions and enhancing the colonization ability in DE456.
Interleukin-2 enhancer binding factor 2 negatively regulates the replication of duck hepatitis A virus type 1 by disrupting the RNA-dependent RNA polymerase activity of 3D polymerase
Authors: An Hao , Yu Xiaoli , Li Jing , Shi Fuyan , Liu Yumei , Shu Ming , Li Zihan , Li Xiaohong , Li Wanwei , Chen Junhao 
Published: March 2024

AbstractThe interaction between viral components and cellular proteins plays a crucial role in viral replication. In a previous study, we showed that the 3′—untranslated region (3′—UTR) is an essential element for the replication of duck hepatitis A virus type 1 (DHAV-1). However, the underlying mechanism is still unclear. To gain a deeper understanding of this mechanism, we used an RNA pull-down and a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry assay to identify new host factors that interact with the 3′—UTR. We selected interleukin-2 enhancer binding factor 2 (ILF2) for further analysis. We showed that ILF2 interacts specifically with both the 3′—UTR and the 3D polymerase (3Dpol) of DHAV-1 through in vitro RNA pull-down and co-immunoprecipitation assays, respectively. We showed that ILF2 negatively regulates viral replication in duck embryo fibroblasts (DEFs), and that its overexpression in DEFs markedly suppresses DHAV-1 replication. Conversely, ILF2 silencing resulted in a significant increase in viral replication. In addition, the RNA-dependent RNA polymerase (RdRP) activity of 3Dpol facilitated viral replication by enhancing viral RNA translation efficiency, whereas ILF2 disrupted the role of RdRP in viral RNA translation efficiency to suppress DHAV-1 replication. At last, DHAV-1 replication markedly suppressed the expression of ILF2 in DEFs, duck embryo hepatocytes, and different tissues of 1 day-old ducklings. A negative correlation was observed between ILF2 expression and the viral load in primary cells and different organs of young ducklings, suggesting that ILF2 may affect the viral load both in vitro and in vivo.
Design and analysis antennas of transverse polarization on the dielectric waveguide
Authors: Shaaban Mohamed N. , Nasybullin Aydar R. , Sedelnikov Yuri E. 
Published: March 2024

AbstractThis paper addresses the issue of constructing millimeter band antennas using dielectric waveguide structures. A new type of linear antenna, incorporating metal pins on the side walls of the grooved dielectric waveguide, is proposed for generating polarization perpendicular to the waveguide axis. However, these antennas suffer from the drawback of cross-polarized radiation in directions close to the waveguide axis. To overcome this limitation, a modified antenna design with transverse polarization is introduced, featuring a closed groove waveguide with a longitudinal slot at the top of the wall. The paper provides a comparison between two types of dielectric waveguide antennas: first, waveguide antenna with grooves in the dielectric which results in longitudinal polarization, and second, waveguide antenna with quarter-wavelength pins which results in transverse polarization. Electrodynamic modeling data are provided to demonstrate the effectiveness of the proposed antennas for satellite, 5G antenna, and radar applications. Finally, an antenna of quarter-wavelength pins is proposed with a frequency of 39 GHz, a gain of 19.8 dBi, and a width of the radiation pattern of 3.2$$^{\circ }$$∘, and a sidelobe level (SLL) of -13.3 dB has been achieved.
On a new version of Hermite–Hadamard-type inequality based on proportional Caputo-hybrid operator
Authors: Tunç Tuba , Demir İzzettin 
Published: March 2024

AbstractIn mathematics and the applied sciences, as a very useful tool, fractional calculus is a basic concept. Furthermore, in many areas of mathematics, it is better to use a new hybrid fractional operator, which combines the proportional and Caputo operators. So we concentrate on the proportional Caputo-hybrid operator because of its numerous applications. In this research, we introduce a novel extension of the Hermite–Hadamard-type inequalities for proportional Caputo-hybrid operator and establish an identity. Then, taking into account this novel generalized identity, we develop some integral inequalities associated with the left-side of Hermite–Hadamard-type inequalities for proportional Caputo-hybrid operator. Moreover, to illustrate the newly established inequalities, we give some examples with the help of graphs.
The gut metabolite indole-3-propionic acid activates ERK1 to restore social function and hippocampal inhibitory synaptic transmission in a 16p11.2 microdeletion mouse model
Authors: Jiang Jian , Wang Dilong , Jiang Youheng , Yang Xiuyan , Sun Runfeng , Chang Jinlong , Zhu Wenhui , Yao Peijia , Song Kun , Chang Shuwen , Wang Hong , Zhou Lei , Zhang Xue-Song , Li Huiliang , Li Ningning 
Published: March 2024

Abstract
Background
Microdeletion of the human chromosomal region 16p11.2 (16p11.2$${}^{+/-}$$

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) is a prevalent genetic factor associated with autism spectrum disorder (ASD) and other neurodevelopmental disorders. However its pathogenic mechanism remains unclear, and effective treatments for 16p11.2$${}^{+/-}$$

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syndrome are lacking. Emerging evidence suggests that the gut microbiota and its metabolites are inextricably linked to host behavior through the gut-brain axis and are therefore implicated in ASD development. Despite this, the functional roles of microbial metabolites in the context of 16p11.2$${}^{+/-}$$

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are yet to be elucidated. This study aims to investigate the therapeutic potential of indole-3-propionic acid (IPA), a gut microbiota metabolite, in addressing behavioral and neural deficits associated with 16p11.2$${}^{+/-}$$

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, as well as the underlying molecular mechanisms.

Results
Mice with the 16p11.2$${}^{+/-}$$

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showed dysbiosis of the gut microbiota and a significant decrease in IPA levels in feces and blood circulation. Further, these mice exhibited significant social and cognitive memory impairments, along with hyperactivation of hippocampal dentate gyrus neurons and reduced inhibitory synaptic transmission in this region. However, oral administration of IPA effectively mitigated the histological and electrophysiological alterations, thereby ameliorating the social and cognitive deficits of the mice. Remarkably, IPA treatment significantly increased the phosphorylation level of ERK1, a protein encoded by the Mapk3 gene in the 16p11.2 region, without affecting the transcription and translation of the Mapk3 gene.

Conclusions
Our study reveals that 16p11.2$${}^{+/-}$$

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leads to a decline in gut metabolite IPA levels; however, IPA supplementation notably reverses the behavioral and neural phenotypes of 16p11.2$${}^{+/-}$$

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mice. These findings provide new insights into the critical role of gut microbial metabolites in ASD pathogenesis and present a promising treatment strategy for social and cognitive memory deficit disorders, such as 16p11.2 microdeletion syndrome.
Medical student wellbeing during COVID-19: a qualitative study of challenges, coping strategies, and sources of support
Authors: West Helen M , Flain Luke , Davies Rowan M , Shelley Benjamin , Edginton Oscar T 
Published: March 2024

Abstract
Background
Medical students face challenges to their mental wellbeing and have a high prevalence of mental health problems. During training, they are expected to develop strategies for dealing with stress. This study investigated factors medical students perceived as draining and replenishing during COVID-19, using the ‘coping reservoir’ model of wellbeing.

Methods
In synchronous interactive pre-recorded webinars, 78 fourth-year medical students in the UK responded to reflective prompts. Participants wrote open-text comments on a Padlet site. Responses were analysed using reflexive thematic analysis.

Results
Analysis identified five themes. COVID-19 exacerbated academic pressures, while reducing the strategies available to cope with stress. Relational connections with family and friends were affected by the pandemic, leading to isolation and reliance on housemates for informal support. Relationships with patients were adversely affected by masks and telephone consultations, however attending placement was protective for some students’ wellbeing. Experiences of formal support were generally positive, but some students experienced attitudinal and practical barriers.

Conclusions
This study used a novel methodology to elicit medical students’ reflections on their mental wellbeing during COVID-19. Our findings reinforce and extend the ‘coping reservoir’ model, increasing our understanding of factors that contribute to resilience or burnout. Many stressors that medical students typically face were exacerbated during COVID-19, and their access to coping strategies and support were restricted. The changes to relationships with family, friends, patients, and staff resulted in reduced support and isolation. Recognising the importance of relational connections upon medical students’ mental wellbeing can inform future support.
Development of indicators and moral intelligence scales for junior high school students: mixed-method research
Authors: Prasertsin Ujsara , Srihaset Kamontip , Roopsuwankun Pattama 
Published: March 2024

AbstractThe purposes of this research were (1) to study the indicators of moral intelligence for junior high school students, (2) to develop moral intelligence scales for junior high school students, and (3) to assess the needs of moral intelligence for junior high school students. By the mixed-method research, the sample group used in the research was 11 key informants for in-depth interviews, 100 students in mixed classes of 20 schools, 1,997 students in total. The results show the following:1. The moral intelligence indicators for junior high school students consisted of 6 indicators: Equality, Empathy, Morality, Tolerance, Self-control, and Kindness.2. To create moral intelligence scales for junior high school students, the Cronbach’s alpha coefficients for each section were.939,0.920 and.841, respectively. With corroborative component analysis classified by the overall composition, it was found that the mean was at a high level (M = 3.955), with a low distribution with a standard deviation (SD) of 0.576 and a distribution coefficient (C.V.) of 14.566.3. For needs assessment of moral intelligence for junior high school students, it was found that the third component of morality had the most needs (PNImodified=0.095), followed by the 5th component of self-control (PNImodified=0.081), the second component of empathy (PNImodified=0.075), the 4th component of tolerance (PNImodified=0.073), the 6th component of kindness (PNImodified=0.055) and the first component of equality (PNImodified=0.053).
The Impact of Servant Leadership on Proactive Service Behavior: a Moderated Mediation Model
Authors: Zhang Yinan , Yin Yue , Su Weilin 
Published: March 2024

AbstractAs the implementers of government policies, junior civil servants bear the responsibility of providing services to the public. Whether they can put themselves in the people’s shoes and show more active service consciousness directly reflects the government’s management ability and the realization of service-oriented government goals. Although proactive service behavior has been studied, it has not been brought into the field of government administration. Hence, from the perspective of servant leadership, this study attempts to introduce proactive service behavior (PSB) into the field of government administration, and discusses the promotion strategies of junior civil servants’ PSB in China. Through the statistical analysis of 416 junior civil servants collected in the two stages, this study verifies that servant leadership has a significant positive impact on public service motivation and PSB of junior civil servants. Public service motivation (PSM) can partially mediate the promotion effect of servant leadership on junior civil servants’ PSB. Role identity can positively moderate the relationship between servant leadership and junior civil servants’ PSM, and then affect their PSB. Therefore, this study suggests that leading cadres should integrate servant leadership into daily life, take the lead in providing quality services to their subordinates, and then inspire more proactive service to the masses.
Feasibility of UV–Vis spectroscopy combined with pattern recognition techniques to authenticate the medicinal plant material from different geographical areas
Authors: Casoni Dorina , Cobzac Simona Codruța Aurora , Simion Ileana Maria 
Published: March 2024

AbstractThe correct identification and authentication of medicinal plants material is a crucial task that ensures quality and prevent adulteration. The use of UV–Vis spectroscopy with principal component analysis (PCA) and discriminant analysis (DA) was proposed for identification/authentication of plant material form different genus and different geographical areas provenience. Hydroalcoholic extracts of samples from twelve genus collected from seven countries (Romania, North Macedonia, Germany, Italy, Serbia, Russia and Kazakhstan) were used. The UV–Vis spectra of the extracts were acquired in the 200–800 nm spectral range, and signal smoothing was used for pre-processing the spectral data. Hierarchical clustering analysis (HCA) with 1-Pearson r distance measurement was used to classify the samples based on the original spectra and different-order derivative spectra, respectively. Data from original spectra and from different-order derivative spectra were evaluated by PCA method. Using the PCA with varimax rotation approach, the spectral ranges with significant contribution for samples classification were revealed for the first time. When the PCA method coupled with DA was applied to the data obtained from the original spectra and the fourth-order derivative spectra, the samples were correctly classified to the respective groups with a 98.04% accuracy. The proposed method can be a useful tool for rapid authentication of plant material derived from different countries.
Beyond performance, competence, and recognition: forging a science researcher identity in the context of research training
Authors: Pfeifer Mariel A. , Zajic C. J. , Isaacs Jared M. , Erickson Olivia A. , Dolan Erin L. 
Published: March 2024

Abstract
Background
Studying science identity has been useful for understanding students’ continuation in science-related education and career paths. Yet knowledge and theory related to science identity among students on the path to becoming a professional science researcher, such as students engaged in research at the undergraduate, postbaccalaureate, and graduate level, is still developing. It is not yet clear from existing science identity theory how particular science contexts, such as research training experiences, influence students’ science identities. Here we leverage existing science identity and professional identity theories to investigate how research training shapes science identity. We conducted a qualitative investigation of 30 early career researchers—undergraduates, postbaccalaureates, and doctoral students in a variety of natural science fields—to characterize how they recognized themselves as science researchers.

Results
Early career researchers (ECRs) recognized themselves as either science students or science researchers, which they distinguished from being a career researcher. ECRs made judgments, which we refer to as “science identity assessments”, in the context of interconnected work-learning and identity-learning cycles. Work-learning cycles referred to ECRs’ conceptions of the work they did in their research training experience. ECRs weighed the extent to which they perceived the work they did in their research training to show authenticity, offer room for autonomy, and afford opportunities for epistemic involvement. Identity-learning cycles encompassed ECRs’ conceptions of science researchers. ECRs considered the roles they fill in their research training experiences and if these roles aligned with their perceptions of the tasks and traits of perceived researchers. ECRs’ identity-learning cycles were further shaped by recognition from others. ECRs spoke of how recognition from others embedded within their research training experiences and from others removed from their research training experiences influenced how they see themselves as science researchers.

Conclusions
We synthesized our findings to form a revised conceptual model of science researcher identity, which offers enhanced theoretical precision to study science identity in the future. We hypothesize relationships among constructs related to science identity and professional identity development that can be tested in further research. Our results also offer practical implications to foster the science researcher identity of ECRs.