bluuebunny/crossref_metadata_2025_split huggingface dataset:
Desert diversity: genome sequence of
Gordonia rubripertincta
cluster DJ phage Mossy and cluster DV phage Erutan
Authors: Barnes October , Workman Christopher J. , Patterson Noah C. , Oesch Riley , Johnson Katie L. , Goncz Kaarin , Sharbrough Joel , DeVeaux Linda C. 
Published: March 2024

ABSTRACT

Lytic bacteriophages Mossy and Erutan were directly isolated from desert soil on
Gordonia rubripertincta
and characterized by their morphologies and genomes. Mossy, part of the DJ cluster of Actinobacteriophage, has a genome of 61,183 bp. The genome of Erutan, part of the DV cluster, is 66,957 bp.
Impact of post-procedure stenosis on outcomes of patients with severe intracranial stenosis treated with intracranial stent placement
Authors: Nunna Ravi S , Ma Xiaoyu , Genovese Sabrina , Lodhi Abdullah , Bains Navpreet K , Cohen David , Smith Caitlyn , Ortiz Michael J , Siddiq Farhan , Gomez Camilo R , Qureshi Adnan I 
Published: March 2024

BackgroundThe optimal target post-procedure stenosis after percutaneous angioplasty and stent placement (PTAS) for intracranial stenosis is unknown. We determined the effect of post-procedure stenosis after intracranial PTAS on subsequent clinical events in patients with severe symptomatic intracranial stenosis.MethodsWe categorized the severity of post-procedure stenosis as ‘<30%’, ‘30–49%’, and ‘≥50%’ among 207 patients who underwent PTAS in a multicenter randomized clinical trial. Outcomes included stroke or death within 72 hours and within 30 days, ipsilateral stroke beyond 30 days of treatment, and stroke or death within 30 days or stroke in the qualifying artery beyond 30 days (primary endpoint of the trial). Cox proportional hazards analysis was performed with adjustments for age, initial severity of stenosis, location of stenosis, and qualifying event. Kaplan-Meier curves were generated for the primary endpoint stratified by post-procedure stenosis with log-rank analysis.ResultsThe severity of post-procedure stenosis was categorized as <30%, 30–49%, and ≥50% in 112, 73, and 22 patients, respectively. Compared with patients with post-procedure stenosis <30%, there was no difference in the risk of primary endpoint among patients with post-procedure stenoses of 30–49% (hazards ratio (HR) 0.85, 95% confidence interval (95% CI) 0.64 to 1.15) or those with ≥50% (HR 0.91, 95% CI 0.57 to 1.43). Log-rank analysis did not demonstrate a difference in rates of primary endpoint between groups stratified by post-procedure stenosis (P=0.70).ConclusionIn the absence of any benefit on short- and long-term outcomes, strategies to achieve a low severity of post-procedure stenosis among patients with severe intracranial stenosis may not be warranted.
Effects of liquid nicotine concentration and flavour on the acceptability of electronic nicotine delivery systems (ENDS) among people who smoke participating in a randomised controlled trial to reduce cigarette consumption
Authors: Yingst Jessica , Midya Vishal , White Augustus , Foulds Jonathan , Cobb Caroline O , Veldheer Susan , Yen Miao-Shan , Eissenberg Thomas 
Published: March 2024

BackgroundResearch is needed to understand the acceptability of electronic nicotine delivery systems (ENDS) as a smoking reduction aid. This study examines the acceptability of ENDS by liquid nicotine concentration and flavour among people who smoke using ENDS to reduce their smoking.MethodsPeople who smoke cigarettes but were naïve to ENDS participated in a double-blind randomised controlled trial to reduce conventional cigarette smoking. Participants were randomised to either a control cigarette substitute (CS) or one of three ENDS groups; 0 mg/mL, 8 mg/mL or 36 mg/mL nicotine concentration. ENDS flavour was chosen by the participant (tobacco or menthol). Participants reported their CS, ENDS and cigarettes per day (CPD) from the past 7 days at 1-month, 3-month and 6-month follow-up visits. Participants also reported side effects and measures of satisfaction, psychological reward, aversion and craving relief. Outcome variables were modelled using linear mixed effects by the following groups: liquid nicotine concentration, flavour and a flavour-nicotine concentration interaction.ResultsParticipants (n=520) were 41.2% male, 67.3% white, had a mean age of 46.2 years and smoked a mean of 18.6 CPD (SD=7.74) at baseline. All flavour and concentration groups decreased CPD from baseline to all follow-up visits with the 36 mg/mL experiencing the greatest reduction, compared with the 0 mg/mL and 8 mg/mL groups. All groups except the 36 mg/mL group decreased their product use over time. The use of menthol flavour was associated with fewer side effects at 3 months (p=0.02) and lesser aversion at 1 month (p=0.03) compared with tobacco-flavoured ENDS. The 36 mg/mL group experienced the greatest craving relief and greatest aversion compared with other groups.ConclusionsBoth nicotine concentration and flavour appear to have independent, as well as interactive, effects that influence ENDS acceptability among people who use cigarettes.
Force convective heat transfer simulation in a rectangular channel with multiple square obstacles via finite element method
Authors: Ashraf Muhammad Waqas , Haider Jamil Abbas , Ghazwani Hassan Ali , Ahmad Shahbaz , Zheng Zhoushun , Gul Sana 
Published: March 2024

This study investigated fluid flow and forced convective heat transfer in rectangular microchannels with square barriers, as illustrated in Fig. 1 . In the first situation, three obstacles were positioned along the microchannel’s top wall. In the second scenario, obstacles were positioned along the microchannel’s bottom wall. In the final example, three square obstacles are placed symmetrically on either side of the microchannel wall. With the help of the Finite Element Method (FEM), we investigate the physicochemical behavior of the microchannel. The development of computer code within COMSOL multiphysics made it possible to simulate heat transport and fluid flow. The results include the implications of the rarefaction effect on fluid flow and heat transmission and decisions regarding the location of barriers and the shape of obstacles in squares. In addition, with the lowest value in skin friction and a lower Nusselt number, the third example, which has barriers on both sides, provides a valuable method for reducing the fluid temperature at the exit of the microchannel. This is because it has barriers on both sides. In the section under “Results and Discussion,” we provide an in-depth analysis of the numerical data derived from the microchannel. • Objective: This study aims to numerically investigate forced convective heat transfer in a rectangular channel featuring multiple square obstacles. The simulation employs the FEM to model fluid flow and temperature distribution. • Geometry and complexity: The channel geometry incorporates square obstacles, introducing geometric complexity. The FEM is chosen for its capability to handle intricate geometries accurately.
Infiltrative Vessel Co-optive Growth Pattern Induced by IQGAP3 Overexpression Promotes Microvascular Invasion in Hepatocellular Carcinoma
Authors: Tang Miaoling , Zhang Shuxia , Yang Meisongzhu , Feng Rongni , Lin Jinbin , Chen Xiaohong , Xu Yingru , Yu Ruyuan , Liao Xinyi , Li Ziwen , Li Xincheng , Li Man , Zhang Qiliang , Chen Suwen , Qian Wanying , Liu Yuanji , Song Libing , Li Jun 
Published: March 2024

Abstract

Purpose:
Microvascular invasion (MVI) is a major unfavorable prognostic factor for intrahepatic metastasis and postoperative recurrence of hepatocellular carcinoma (HCC). However, the intervention and preoperative prediction for MVI remain clinical challenges due to the absent precise mechanism and molecular marker(s). Herein, we aimed to investigate the mechanisms underlying vascular invasion that can be applied to clinical intervention for MVI in HCC.

Experimental Design:
The histopathologic characteristics of clinical MVI+/HCC specimens were analyzed using multiplex immunofluorescence staining. The liver orthotopic xenograft mouse model and mechanistic experiments on human patient-derived HCC cell lines, including coculture modeling, RNA-sequencing, and proteomic analysis, were used to investigate MVI-related genes and mechanisms.

Results:
IQGAP3 overexpression was correlated significantly with MVI status and reduced survival in HCC. Upregulation of IQGAP3 promoted MVI+-HCC cells to adopt an infiltrative vessel co-optive growth pattern and accessed blood capillaries by inducing detachment of activated hepatic stellate cells (HSC) from the endothelium. Mechanically, IQGAP3 overexpression contributed to HCC vascular invasion via a dual mechanism, in which IQGAP3 induced HSC activation and disruption of the HSC–endothelial interaction via upregulation of multiple cytokines and enhanced the trans-endothelial migration of MVI+-HCC cells by remodeling the cytoskeleton by sustaining GTPase Rac1 activity. Importantly, systemic delivery of IQGAP3-targeting small-interfering RNA nanoparticles disrupted the infiltrative vessel co-optive growth pattern and reduced the MVI of HCC.

Conclusions:
Our results revealed a plausible mechanism underlying IQGAP3-mediated microvascular invasion in HCC, and provided a potential target to develop therapeutic strategies to treat HCC with MVI.
Experimental Estimates of College Coaching on Postsecondary Reenrollment
Authors: Turner Lesley J. , Gurantz Oded 
Published: March 2024

Abstract
College attendance has increased significantly over the last few decades, but dropout rates remain high, with fewer than half of all adults ultimately obtaining a postsecondary credential. This project investigates whether one-on-one college coaching improves college attendance and completion outcomes for former income state aid recipients identified as low- and middle-income who attended college but left prior to earning a degree. We conducted a randomized control trial with approximately 8,000 former students in their early to mid 20s. Half of the participants assigned to the treatment group were offered the opportunity to receive coaching services from InsideTrack, with all communication done remotely via phone or video. Intent-to-treat analyses based on assignment to coaching show no impacts on college enrollment and we can rule out effects larger than a 2 percentage point (5 percent) increase in subsequent Fall enrollment.
Give Due Consideration …
Authors: Ernst Gisela , Stöger Karl 
Published: March 2024

Abstract
The new EU Regulation on health technology assessment (HTAR) provides for joint clinical assessments (JCA) of health technologies at EU level. When Member States carry out health technology assessments (HTA) at the national level, they shall give due consideration to the results of a JCA and comply with other obligations of the Regulation. This article aims to clarify what these obligations mean for the Member States and whether JCA results have to be considered outside a national health technology assessment as well. In this context, the question of which processes qualify as ‘national HTA’ and which requirements need to be fulfilled to trigger the obligations under Article 13 HTAR are discussed in more detail in this paper.
The Great Kanto Earthquake Ephemera Collection at Princeton University Library
Authors: Noguchi Setsuko 
Published: March 2024

Abstract
The East Asian Library at Princeton contains a substantial collection of books, journals, photographs and ephemera related to the Great Kanto Earthquake of 1923. In this article I introduce the more ephemeral items, including government notifications, posters, maps and flyers. Amongst them are flyers concerning lost children, notices about free accommodation for pregnant woman and exhortations not to believe rumors. Many of these items have now been digitalized and they constitute a valuable research collection.
Assessing the Right to Freedom of Peaceful Assembly in Post-Pandemic, Post-Brexit Britain through the Lens of the 2022–2023 Public Sector Strikes
Authors: Masters Mercedes 
Published: March 2024

Abstract
Britain is currently seeing the most coordinated public sector strike action since the 1970s. In a post-pandemic, post-Brexit Britain, the general public are going through a cost-of-living crisis, with the rate of inflation rising and real-term pay cuts. This, paired with growing restrictions on the right of peaceful assembly through the passing of oppressive policing bills, means that people are increasingly turning to unions and striking to get their voices heard. This article highlights the push-factors leading to the 2022–2023 Public Sector Strikes through a socio-political analysis of democracy and power in post-pandemic, post-Brexit Britain, whilst capturing the voices of those striking and on picket lines.
Taking the Bull by His Horn: Augustus Slays the Mithraic Bull
Authors: Mazhjoo Nina 
Published: March 2024

Abstract
The Mithraic visual language can be read as a hybrid iconography created by the Roman Mithraists to stress the non-Roman provenance of their deity. This note analyses the gesture of Mithras’ slaying the bull and the so-called “Mithraic hold” to reveal the ex-novo nature of the Mithraic visual language and iconography from two vantage points: the first, at the aesthetic level; and the second, from its sources and origin. Additional considerations are also given on the iconographical referent of the Mithraic bull as well as the numismatic symbols traditionally discussed in relation to the Mithraic visual language.
Thoughts on the Iconography of the Sophytos Coinage
Authors: Härtel Stefan 
Published: March 2024

Abstract
The iconography of the coinage issued by Sophytos in early Hellenistic Bactria is analysed. It has long been recognised that the iconography involving a cockerel, winged cheek-guards and an “open 8” caduceus suggests the invocation of the Greek god Hermes. Taking into account the Iranian context of the coinage, the possibility is analysed that the coinage testifies an identification of Hermes with Sraoša. While the evidence is wanting, such an interpretatio seems well possible. Independent of this, the coinage is put into context with the mention of a cockerel used as a field ensign in the Achaemenid period by Plutarch. It suggests that rather than interpret Sophytos as a “rebel” against Macedonian rule in Bactria, he should be considered a governor who derived his legitimacy from an imperial Achaemenid or Macedonian office.
Assessing Regional-Scale Heterogeneity in Blue–Green Water Availability under the 1.5°C Global Warming Scenario
Authors: Tyagi Shoobhangi , Sahany Sandeep , Saraswat Dharmendra , Mishra Saroj Kanta , Dubey Amlendu , Niyogi Dev 
Published: March 2024

Abstract
The 2015 Paris Agreement outlined limiting global warming to 1.5°C relative to the preindustrial levels, necessitating the development of regional climate adaptation strategies. This requires a comprehensive understanding of how the 1.5°C rise in global temperature would translate across different regions. However, its implications on critical agricultural components, particularly blue and green water, remains understudied. This study investigates these changes using a rice-growing semiarid region in central India. The aim of this study is to initiate a discussion on the regional response of blue–green water at specific warming levels. Using different global climate models (GCMs) and shared socioeconomic pathways (SSPs), the study estimated the time frame for reaching the 1.5°C warming level and subsequently investigated changes in regional precipitation, temperature, surface runoff, and blue–green water. The results reveal projected reductions in precipitation and surface runoff by approximately 5%–15% and 10%–35%, respectively, along with decrease in green and blue water by approximately 12%–1% and 40%–10%, respectively, across different GCMs and SSPs. These findings highlight 1) the susceptibility of blue–green water to the 1.5°C global warming level, 2) the narrow time frame available for the region to develop the adaptive strategies, 3) the influence of warm semiarid climate on the blue–green water dynamics, and 4) the uncertainty associated with regional assessment of a specific warming level. This study provides new insights for shaping food security strategies over highly vulnerable semiarid regions and is expected to serve as a reference for other regional blue/green water assessment studies.

Significance Statement
This study helps to drive home the message that a global agreement to limit the warming level to 1.5°C does not mean local-scale temperature (and associated hydrological) impacts would be limited to those levels. The regional changes can be more exaggerated and uncertain, and they also depend on the choice of the climate model and region. Therefore, local-scale vulnerability assessments must focus on the multidimensional assessment of a 1.5°C warmer world involving different climate models, climate-sensitive components, and regions. This information is relevant for managing vulnerable agricultural systems. This study is among the first to investigate the critical agricultural components such as the blue–green water over a semiarid Indian region, and the findings and methodology are expected to be transferable for performing regional-scale assessments elsewhere.
Accuracy of maximal acceleration time of pedal arteries to diagnose critical limb-threatening ischemia
Authors: Trihan Jean-Eudes , Croquette Magali , Hersant Jeanne , Prigent Romain , Fontaine Cedric , Henni Samir , Lanéelle Damien 
Published: March 2024

Introduction: Maximal acceleration time of distal arteries of the foot (ATmax) is correlated to ankle–brachial index (ABI) and toe–brachial index (TBI), and seems very promising in diagnosing severe peripheral artery disease (PAD) and especially critical limb-threatening ischemia (CLTI). Our goal was to confirm the cut-off value of 215 ms to predict a toe pressure (TP) ⩽ 30 mmHg. Methods: A 4-month retrospective study was conducted on patients addressed for suspicion of PAD. Demographic data, ABI, TBI, and Doppler ultrasound scanning parameters of the dorsal pedis and lateral plantar arteries (DPA and LPA) were recorded. Results: A total of 137 patients with 258 lower limbs were included. ATmax was highly correlated to TBI ( r = −0.89, p < 0.001). With the cut-off value of 215 ms, ATmax was effective to diagnose TP ⩽ 30 mmHg with a sensitivity of 93% [95% CI 77–99], a specificity of 96% [95% CI 92–98], a positive predictive value of 73% [95% CI 56–86], a negative predictive value of 99% [95% CI 97–100], and an area under the receiver operating characteristics curve of 0.99 [95% CI 0.98–1.00]. ATmax also showed promising results to rule out PAD in healthy patients. Conclusion: ATmax is a reliable diagnostic tool to diagnose low TP and could be a new easily performed hemodynamic criterion for diagnosis of CLTI.
Combining GWAS and comparative genomics to fine map candidate genes for days to flowering in mung bean
Authors: Chiteri Kevin O. , Rairdin Ashlyn , Sandhu Kulbir , Redsun Sven , Farmer Andrew , O’Rourke Jamie A. , Cannon Steven B. , Singh Arti 
Published: March 2024

Abstract
Background
Mung bean (Vigna radiata (L.) Wilczek), is an important pulse crop in the global south. Early flowering and maturation are advantageous traits for adaptation to northern and southern latitudes. This study investigates the genetic basis of the Days-to-Flowering trait (DTF) in mung bean, combining genome-wide association studies (GWAS) in mung bean and comparisons with orthologous genes involved with control of DTF responses in soybean (Glycine max (L) Merr) and Arabidopsis (Arabidopsis thaliana).

Results
The most significant associations for DTF were on mung bean chromosomes 1, 2, and 4. Only the SNPs on chromosomes 1 and 4 were heavily investigated using downstream analysis. The chromosome 1 DTF association is tightly linked with a cluster of locally duplicated FERONIA (FER) receptor-like protein kinase genes, and the SNP occurs within one of the FERONIA genes. In Arabidopsis, an orthologous FERONIA gene (AT3G51550), has been reported to regulate the expression of the FLOWERING LOCUS C (FLC). For the chromosome 4 DTF locus, the strongest candidates are Vradi04g00002773 and Vradi04g00002778, orthologous to the Arabidopsis PhyA and PIF3 genes, encoding phytochrome A (a photoreceptor protein sensitive to red to far-red light) and phytochrome-interacting factor 3, respectively. The soybean PhyA orthologs include the classical loci E3 and E4 (genes GmPhyA3, Glyma.19G224200, and GmPhyA2, Glyma.20G090000). The mung bean PhyA ortholog has been previously reported as a candidate for DTF in studies conducted in South Korea.

Conclusion
The top two identified SNPs accounted for a significant proportion (~ 65%) of the phenotypic variability in mung bean DTF by the six significant SNPs (39.61%), with a broad-sense heritability of 0.93. The strong associations of DTF with genes that have orthologs with analogous functions in soybean and Arabidopsis provide strong circumstantial evidence that these genes are causal for this trait. The three reported loci and candidate genes provide useful targets for marker-assisted breeding in mung beans.
Shared and distinct interactions of type 1 and type 2 Epstein-Barr Nuclear Antigen 2 with the human genome
Authors: Viel Kenyatta C. M. F. , Parameswaran Sreeja , Donmez Omer A. , Forney Carmy R. , Hass Matthew R. , Yin Cailing , Jones Sydney H. , Prosser Hayley K. , Diouf Arame A. , Gittens Olivia E. , Edsall Lee E. , Chen Xiaoting , Rowden Hope , Dunn Katelyn A. , Guo Rui , VonHandorf Andrew , Leong Merrin Man Long , Ernst Kevin , Kaufman Kenneth M. , Lawson Lucinda P. , Gewurz Ben , Zhao Bo , Kottyan Leah C. , Weirauch Matthew T. 
Published: March 2024

Abstract
Background
There are two major genetic types of Epstein-Barr Virus (EBV): type 1 (EBV-1) and type 2 (EBV-2). EBV functions by manipulating gene expression in host B cells, using virus-encoded gene regulatory proteins including Epstein-Barr Nuclear Antigen 2 (EBNA2). While type 1 EBNA2 is known to interact with human transcription factors (hTFs) such as RBPJ, EBF1, and SPI1 (PU.1), type 2 EBNA2 shares only ~ 50% amino acid identity with type 1 and thus may have distinct binding partners, human genome binding locations, and functions.

Results
In this study, we examined genome-wide EBNA2 binding in EBV-1 and EBV-2 transformed human B cells to identify shared and unique EBNA2 interactions with the human genome, revealing thousands of type-specific EBNA2 ChIP-seq peaks. Computational predictions based on hTF motifs and subsequent ChIP-seq experiments revealed that both type 1 and 2 EBNA2 co-occupy the genome with SPI1 and AP-1 (BATF and JUNB) hTFs. However, type 1 EBNA2 showed preferential co-occupancy with EBF1, and type 2 EBNA2 preferred RBPJ. These differences in hTF co-occupancy revealed possible mechanisms underlying type-specific gene expression of known EBNA2 human target genes: MYC (shared), CXCR7 (type 1 specific), and CD21 (type 2 specific). Both type 1 and 2 EBNA2 binding events were enriched at systemic lupus erythematosus (SLE) and multiple sclerosis (MS) risk loci, while primary biliary cholangitis (PBC) risk loci were specifically enriched for type 2 peaks.

Conclusions
This study reveals extensive type-specific EBNA2 interactions with the human genome, possible differences in EBNA2 interaction partners, and a possible new role for type 2 EBNA2 in autoimmune disorders. Our results highlight the importance of considering EBV type in the control of human gene expression and disease-related investigations.