bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feeding value of enset (Ensete ventricosum), Desmodium intortum hay and untreated or urea and calcium oxide treated wheat straw for sheep
Authors: Nurfeta A. , Tolera A. , Eik L. O. , Sundstøl F. 
Published: March 2008

SummaryFeed intake, in vivo nutrient digestibility and nitrogen utilization were evaluated in male sheep fed different fractions (leaf, pseudostem, corm, whole plant) of enset, untreated or 2% urea‐ and 3% calcium oxide‐ (CaO or lime) treated wheat straw and Desmodium intortum hay as sole diets. All feeds, except D. intortum hay and enset leaf had low crude protein (CP) content. Non‐fiber carbohydrate contents were higher in enset fractions, especially in pseudostem and corm relative to other feeds. Enset leaf and pseudostem had high calcium, phosphorus and manganese contents. Corm, whole enset and D. intortum hay were rich sources of zinc. Daily dry matter and CP intakes were higher (p < 0.05) in sheep fed D. intortum hay (830 and 133 g, respectively) than those fed pseudostem (92 and 7.8 g, respectively). Organic matter digestibilities were highest for corm (0.780) and whole enset (0.776) and lowest for D. intortum hay (0.534) and untreated wheat straw (0.522). The CP digestibility ranged from 0.636 in D. intortum hay to 0.408 in corm. Nitrogen (N) balance was highest (p < 0.05) in D. intortum hay (10.4 g/day) and lowest in corm (−1.3 g/day). Enset leaf could be a useful protein supplement whereas the pseudostem and corm could be good sources of energy.
Influence of rumen escape starch on pancreatic exocrine secretion of goats
Authors: Xu M. , Du S. , Wang J. , Yu Z. P. , Harmon D. L. , Yao J. H. 
Published: March 2008

SummaryThe objective of the experiment was to evaluate the effect of rumen escape starch (RES), accomplished by altering dietary starch concentrations on pancreatic exocrine secretion of goats. Four goats (36.8 ± 3.2 kg) with common bile duct re‐entrant and duodenal catheters were used in a 4 × 4 Latin square. Goats were fed diets containing 20%, 30%, 40% and 50% starch. Periods consisted of 10 day adaptation followed by 3 day of sample collection. Juice was collected in 1‐h fractions continuously for 72 h. Total juice secreted was recorded, and 3% sub samples were retained to form a composite sample. The remaining fluid was returned to the duodenum. Juice composite samples were analyzed for activities of α‐amylase, trypsin, chymotrypsin and lipase. Secretion of pancreatic α‐amylase was lower (p < 0.05) when comparing lambs fed 20% starch diet with 30%, 40% and 50% starch diets. Lipase secretion was greater (p < 0.05) in lambs fed 40% starch diet compared with the other diets. Total secretion of juice, trypsin and chymotrypin was not affected (p > 0.05) by dietary starch concentration. Rumen escape starch increased with increasing dietary starch concentration (p < 0.05). Regression analysis showed that increasing RES results in a quadratic increase (p < 0.05) in pancreatic α‐amylase and lipase secretion, and the secretion of α‐amylase and lipase is maximum when RES is 113 and 83 g/day respectively. These results suggest that optimal RES for pancreatic secretion of α‐amylase and lipase is 80–110 g/day in adult goats.
Differences in biological and behavioural characteristics of Harmonia axyridis (Coleoptera: Coccinellidae) according to colour patterns of elytra
Authors: Seo M. J. , Kim G. H. , Youn Y. N. 
Published: March 2008

AbstractSamples of overwintering populations of Harmonia axyridis were classified into five colour patterns, succinea 1, succinea 2, conspicua, spectabilis and axyridis. Generally, the succinea 1 colour pattern was dominant at six collecting sites in Daejon and Chungnam provinces of Korea and their occurrence proportion was over 50%. Non‐melanic forms such as succinea 1 and 2 were noted in about 90% of collected individuals. On the other hand, conspicua and spectabilis as melanic forms were dominant in the laboratory. One hypothesis is that the difference between field and laboratory populations is related to non‐random mating and environmental conditions. Practically, in mate‐choice experiments, most colour pattern ladybirds seemed to mate with the melanic form, especially the conspicua form. The body size (length) of H. axyridis in colour patterns was slightly different, but in females, there was no significant difference among colour patterns. However, both lengths of males were significantly different among colour patterns. In reproductive ability (fecundity), there was no difference among colour patterns.
Validation of a novel resistance monitoring technique for corn rootworm (Coleoptera: Chrysomelidae) and event DAS‐59122‐7 maize
Authors: Nowatzki T. M. , Lefko S. A. , Binning R. R. , Thompson S. D. , Spencer T. A. , Siegfried B. D. 
Published: March 2008

AbstractRootworm control tactics have recently expanded to include transgenic maize, which express insecticidal proteins from Bacillus thuringiensis (Bt) to reduce larval injury and protect yield potential. Exceptional root protection, increased grower efficiency and improved safety have led to rapid adoption of this technology in the USA. As a result, there is a recognized need for resistance management programmes aimed at delaying rootworm resistance. An essential component of resistance management programmes is the development and implementation of effective resistance monitoring techniques. Five test populations of Diabrotica virgifera virgifera (LeConte) were used to evaluate the sensitivity of two techniques used to describe population susceptibility to the Bt proteins expressed in event DAS‐59122‐7 maize: a diet bioassay employing purified proteins applied to artificial diet and a novel technique using sub‐lethal measures of larval development on seedling maize. Test populations included Rochelle‐US, an unselected susceptible colony, three populations composed of 5%, 25% or 50% Rochelle‐S mixed with Rochelle‐US, and the Rochelle‐S selected colony. Rochelle‐S was derived from the same founding population as Rochelle‐US, but selected for survival on DAS‐59122‐7 maize. Selections identified a minor trait conferring increased tolerance, and greenhouse plant efficacy evaluations confirmed that after 10 generations of selection with no random mating, Rochelle‐S caused significantly more root injury to DAS‐59122‐7 than Rochelle‐US. Rochelle‐S present at 5% of the test population resulted in measurable but not significant increase in injury to DAS‐59122‐7 maize. The diet bioassay was relatively insensitive to the susceptibility differences between the Rochelle‐US and Rochelle‐S populations. Neither LC50 nor EC50 estimates produced statistically significant differentiation between test populations with 0%, 5%, 25% or 50% Rochelle‐S. The sub‐lethal assay clearly identified differences between Rochelle‐S and Rochelle‐US and an increased rate of larval development was measurable when the test population contained only 5% of Rochelle‐S.
Identification and Characterization of a Potyvirus Isolated from Siratro Plants
Authors: Regatieri L. J. , Gaspar J. O. , Belintani P. , Yuki V. A. 
Published: March 2008

AbstractThe present work describes the identification and characterization of a potyvirus isolated from siratro (Macroptilium atropurpureum Urb.) in the north‐west region of the State of São Paulo, Brazil. The virus was transmitted by mechanical inoculation. Its host range was restricted mainly to members of the Fabaceae. A cDNA fragment of about 930 bp was amplified by RT/PCR, cloned and sequenced. The fragment, which included the coat protein gene, had amino acid identity percentages between 88 and 98% with isolates of Bean common mosaic virus (BCMV). Phylogenetic analysis grouped the siratro potyvirus and BCMV isolates in 99% of the replicates, including Azuki mosaic virus, Dendrobium mosaic virus, Blackeye cowpea mosaic virus and Peanut stripe virus, which have been classified as BCMV strains. This is the first citation on the presence of BCMV in siratro plants in Brazil.
Adaptation of the Forecasting Model DOWNCAST for Determination of Downy Mildew Epidemics of Oilseed Poppy in Tasmania, Australia
Authors: Scott J. B. , Hay F. S. , Wilson C. R. 
Published: March 2008

Abstract Peronospora cristata, causing downy mildew, has become an economically important pathogen of oilseed poppy (Papaver somniferum) since its first record in Tasmania in 1996. DOWNCAST, originally developed for downy mildew of onion, was evaluated for its ability to predict sporulation and infection of poppy by P. cristata. To assess the influence of the parameters within DOWNCAST, two modifications (POPCAST1 and POPCAST2) of the standard DOWNCAST model were also developed and tested. Initial accuracy of determination of sporulation events by DOWNCAST was 75%, while POPCAST1 (but not POPCAST2) was a significantly more accurate predictor of sporulation events (86%) than DOWNCAST (χ2 = 5.74, P = 0.025). This increase in accuracy was attributed to increasing both the rainfall threshold inhibitory to sporulation from 0.2 to 3.0 mm, and the relative humidity threshold for induction and development of sporulation from 95% to 96%. While DOWNCAST, POPCAST1 and POPCAST2 had accuracies of infection determination of 65%, 80% and 75%, respectively, chi‐squared analysis indicated no significant difference between models. Preliminary field evaluation indicated that POPCAST1 and POPCAST2 were more sensitive in detecting early infection events than DOWNCAST. Field observations indicated that conditions are frequently conducive for downy mildew spread, therefore the ability to predict early season infection events would allow growers to reduce initial infection rates, and thus inoculum loads later in the season. POPCAST1 and POPCAST2 represent a significant step towards the development of a predictive model for downy mildew in poppy crops.
Identification of AFLP markers linked to the epistatic suppressor gene of a recessive genic male sterility in rapeseed and conversion to SCAR markers
Authors: Xie Y. Z. , Hong D. F. , Xu Z. H. , Liu P. W. , Yang G. S. 
Published: March 2008

AbstractA recessive epistatic genic male sterility (REGMS) two‐type line, 9012AB, has been used for rapeseed hybrid seed production in China. The male sterility of 9012AB is controlled by two recessive duplicate sterile genes (ms1 and ms2) interacting with one recessive epistatic suppressor gene (esp). Homozygosity at the esp locus (espesp) suppresses the expression of the recessive male sterility trait in homozygous ms1ms1ms2 ms2 plants. In this study, we used a combination of bulked segregant analyses and amplified fragment length polymorphism (AFLP) to identify markers linked to the suppressor gene in a BC1 population. From the survey of 1024 AFLP primer combinations, eight markers tightly linked to the target gene were identified. The two closest markers flanking both sides of Esp, P9M5370 and S16M14780, had a genetic distance of 1.4 cM and 2.1 cM, respectively. The AFLP fragment from P4M8190, which co‐segregated with the target gene was converted into a sequence characterized amplified region marker. The availability of linked molecular markers will facilitate the utilization of REGMS in hybrid breeding in Brassica napus.
Development of two multiplex PCR assays targeting improvement of bread‐making and noodle qualities in common wheat
Authors: Zhang X. K. , Liu L. , He Z. H. , Sun D. J. , He X. Y. , Xu Z. H. , Zhang P. P. , Chen F. , Xia X. C. 
Published: March 2008

AbstractWheat quality properties are genetically determined by the compositions of high and low molecular weight glutenin subunits, grain hardness, polyphenol oxidase (PPO) activity and starch viscosity. Two multiplex PCR assays were developed and validated using 70 cultivars and advanced lines from Chinese autumn‐sown wheat regions. Multiplex PCR I includes molecular markers for genes/loci ω‐secalin, Glu‐B1‐2a (By8), Glu‐D1‐1d (Dx5), Glu‐A3d, Glu‐B3 (for non‐1B·1R type) and Pinb‐D1b targeting improved gluten parameters and pan bread quality. Multiplex PCR II comprises markers for genes/loci Ppo‐A1, Ppo‐D1 and Wx‐B1b targeting improved noodle quality. The results were consistent with those achieved by SDS‐PAGE and RP‐HPLC, indicating that the two multiplex assays were highly effective, with good repeatability and low costs enabling their use in wheat breeding programmes. In total, nine alleles (subunits) at locus Glu‐B1, four at Glu‐D1 and five at Glu‐A3 locus were identified, and the alleles (subunits) Glu‐B1b (7 + 8), Glu‐B1c (7 + 9), Glu‐D1a (2 + 12), Glu‐D1d (5 + 10), Glu‐A3a, Glu‐A3c and Glu‐A3d were most frequently present in the cultivars and lines tested. The 1B·1R translocation was present in 28 (40.0%) lines, whereas the Wx‐B1 null allele for better noodle quality was present in only seven (10.0%) cultivars and advanced lines, and 37 (52.9%) lines had Pinb‐D1b associated with hard grains. The allele Ppo‐A1b on chromosome 2AL associated with lower PPO activity was present in 38 (54.3%) genotypes, whereas the less effective allele Ppo‐D1a on chromosome 2DL, also associated with low PPO activity was present in 45 (64.3%) of genotypes. These two multiplex PCR assays should be effective in marker assisted selection targeting improved pan bread‐making and noodle qualities.
Characterizations and molecular mapping of a novel dominant semi‐dwarf gene Sdd(t) in rice (Oryza sativa)
Authors: Liu B. M. , Wu Y. J. , Fu X. D. , Qian Q. 
Published: March 2008

AbstractBy nitrogen ion implanting, we obtained a semi‐dwarf mutant from a japonica rice cultivar Y98148, designated as Y98149. The genetic analysis of Y98149 indicated that the semi‐dwarf phenotype was controlled by a single dominant gene, Sdd(t). We show that Y98149 reduced plant height mainly via inhibiting the first, second and third internode elongation. Based on this dwarfing pattern, the mutant could be grouped into dn‐type dwarf defined by Takeda [Gamma Field Symp. (1977) Vol. 16, PP. 1–18]. In addition, the Sdd(t) gene was sensitive to gibberellin (GA) based on the response to extraneous GA3 and the quantitative determination of endogenous GA1 and GA4. To map the Sdd(t) gene, we tested molecular markers by bulk segregant analysis. The Sdd(t) gene was localized to a 6.4 cM‐interval on the short arm of chromosome 6, flanked by two sequence‐tagged site markers S9 and S13.
Dissection of genetic variance of fibre quality in advanced generations from an interspecific cross of Gossypium hirsutum and G. barbadense
Authors: He D. H. , Lin Z. X. , Zhang X. L. , Zhang Y. X. , Li W. , Nie Y. C. , Guo X. P. 
Published: March 2008

AbstractThe interspecific genetic introgression approach has been shown to facilitate the detection and dissection of quantitative trait loci (QTL). A population consisting of 121 F6 recombinant inbred lines was developed by crossing Gossypium hirsutum cv. ‘Handan 208’ and G. barbadense cv. ‘Pima 90’ via single‐seed descent. Genotyping indicated that the mean ‘Pima 90’ allele frequency at each locus was 21%. Phenotyping and phenotypic distribution indicated a trend of return of individual lines’ characters to ‘Handan 208’ coupled with a wide variance for each trait. Significant loci influencing fibre quality were detected by one‐way analysis of variance (anova; P < 0.005) and association analysis [−log10(P) ≥ 3]: five and three markers for fibre length, four and one marker(s) for uniformity, two and one marker(s) for micronaire, 13 and 15 markers for strength, six and 10 markers for elongation, respectively. Two‐way anova based on genotypes of all marker loci combination showed that 807 two‐locus combinations were significant, and two‐way anova based on marker genotypes of QTL markers combination showed five significant digenic interactions (P < 0.01).
Association analysis of fibre traits in Gossypium arboreum accessions
Authors: Kantartzi S. K. , Stewart J. McD. 
Published: March 2008

AbstractAdvances in the use of diploid Asiatic species in cotton breeding require an understanding of the relatedness and ancestry of diploid cotton accessions, and identification of simple sequence repeat (SSR) markers associated with agronomically important phenotypic traits, for example, fibre quality. Fifty‐six Gossypium arboreum germplasm accessions introduced from nine regions of Africa, Asia and Europe were evaluated for eight fibre characters (lint percentage, lint colour, elongation, micronaire, strength, 50% span length, 2.5% span length and maturity%) and genotyped with 98 SSR markers. When viewed across all accessions most of the SSR markers were polymorphic. Population structure analysis identified six main clusters for the accessions which corresponded to different geographic regions, indicating agreement between genetic and predefined populations. The general linear model method was used to disclose marker–trait associations. Marker–trait associations were investigated by fitting single marker regression models for phenotypic traits on marker band intensities with correction for population structure. This paper illustrates the potential of association mapping in diploid cotton, because existing phenotypic data, a modest number of SSR markers, and a pioneering statistical analysis, identified interesting associations.
Selection of medical students and the maldistribution of the medical workforce in Queensland, Australia
Authors: CRAIG MARK L. , JACKEL CHERYL A. , GERRITS PETRA B. 
Published: March 2008

ABSTRACT: The relative shortage of both general practitioners and specialists providing medical services to rural areas disadvantages people living in the country. This maldistribution of the medical workforce is due in part to the medical education system. The selection and socialisation of medical students, the adequacy of training for rural practice and the continuing support of rural doctors must all be addressed. While progress is being made in vocational training and continuing support of rural practice, aspects of undergraduate education continue to contribute to the shortage of rural practitioners.The school and home addresses of 1991 school leavers in the 1992 intake into first year medicine at the University of Queensland were analysed and compared with those previously reported. Results indicated that students from rural backgrounds remain underrepresented despite previous recommendations to redress the disproportionately low number of rural students entering medical school. Changes to the selection of medical students aimed at increasing the number of rural students entering the course are discussed. This issue needs to be addressed in conjunction with many other factors contributing to the maldistribution of general practitioners if the shortage of rural medical practitioners is to be ultimately corrected.
ROLE OF Ca2+ STORES IN ACETYLCHOLINE‐INDUCED ALL‐OR‐NONE SHORTENING OF SMOOTH MUSCLE CELLS FROM GUINEA‐PIG TAENIA CAECUM
Authors: Mita Mitsuo , Toguchi‐Senrui Natsuko 
Published: March 2008

SUMMARY

We have reported previously that isolated single smooth muscle cells from guinea‐pig taenia caecum respond to acetylcholine (ACh) in an all‐or‐none manner.
To clarify the roles of intracellular Ca2+ stores in the all‐or‐none response of isolated smooth muscle cells from guinea‐pig taenia caecum to ACh, we examined the inositol 1,4,5‐trisphosphate (IP3)‐induced contractile response in Staphylococcus aureus a‐toxin‐permeabilized smooth muscle cells and the effect of depletion of intracellular Ca2+ stores on the all‐or‐none response to ACh in intact smooth muscle cells.
a‐Toxin‐permeabilized smooth muscle cells responded to 3–30 nmol/L or 0.3–3 nmol/L IP3 in the presence of 0.2 mmol/L Ca2+ with 1 mmol/L EGTA or 0.1 mmol/L EGTA, respectively, in an all‐or‐none manner. These results suggest that Ca2+ release induced by IP3 is Ca2+ dependent and is evoked in an all‐or‐none manner.
In the presence of the Ca2+ ionophore A23187 (0.1 mmol/L) or the sarcoplasmic reticulum Ca2+‐ATPase inhibitor cyclopiazonic acid (1 mmol/L), the shortening of intact smooth muscle cells induced by increasing concentrations of ACh showed a graded response, but not an all‐or‐none response.
In conclusion, the results suggest that Ca2+ release from Ca2+ stores induced by IP3 plays an important role in the all‐or‐none response of intact smooth muscle cells to ACh.
METFORMIN, ARTERIAL FUNCTION, INTIMA–MEDIA THICKNESS AND NITROXIDATION IN METABOLIC SYNDROME: THE MEFISTO STUDY
Authors: Meaney Eduardo , Vela Agustín , Samaniego Virginia , Meaney Alejandra , Asbún Juan , Zempoalteca Juan‐Carlos , Elisa Zárate N , Emma Mendoza N , Guzman Martin , Hicks Juan , Ceballos Guillermo 
Published: March 2008

SUMMARY

Metabolic syndrome (MS) is one of the greatest public health problems in Mexico, where more than 75% of adults in urban populations are overweight or obese. Metabolic syndrome has several comorbidities, which result in a high cardiometabolic risk.
Some of the vasopathogenic phenomena in MS are caused by nitroxidant stress, secondary to cardiometabolic dysfunction.
The action of metformin to diminish or control MS remains a matter of debate.
In the present study, 60 patients with at least three diagnostic criteria for MS were divided into two groups. Both groups received similar dietary counselling, but one group was given 850 mg metformin daily.
The variables assessed were body mass index, waist circumference, systolic and diastolic blood pressures (SBP and DBP, respectively), total cholesterol (TC), high‐ and low‐density lipoprotein–cholesterol, triglycerides (TG), fasting glucose, nitroxidant metabolites (free carbonyls, malondialdehyde, dityrosines and advanced oxidative protein products (AOPP)), nitric oxide (NO), carotid vascular stiffness, carotid intima–media thickness (IMT) and C‐reactive protein (CRP).
After 1 year follow up, both groups reported weight loss, as well as decreases in waist circumference, SBP and DBP.
Patients on metformin exhibited reductions in TC and IMT and there were marked changes in nitroxidation: levels of carbonyls, dityrosines and AOPP were reduced, whereas those of NO were increased, indicating better endothelial function. In addition, in patients given metformin, CRP levels decreased.
In conclusion, metformin has a considerable beneficial effect on nitroxidation, endothelial function and IMT in patients with MS.