bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parental responses to children’s early health disadvantages: evidence from a British twin study
Authors: García-Sierra Alicia 
Published: March 2024

Abstract
Health problems experienced in the early years of life have detrimental consequences for the entire life course. However, parents can, through their child-rearing actions, alleviate or aggravate these effects. This article examines how parents respond to the early physical health disadvantages suffered by their children and whether parents from high- and low-socioeconomic backgrounds develop different responses to their children’s early health problems. Using longitudinal data from the Twins Early Development Study, I implement a series of within-twin fixed-effects models and find that, on average, parents develop more negative emotional responses and implement harsher discipline behaviours when their children experience an early health problem. Surprisingly, the effect of health problems on parental responses does not differ by the socioeconomic status of the family. With some nuances, this evidence suggests that parental responses reinforce early-in-life health disadvantages.
Seafood loss prevention and waste reduction
Authors: Conz Andrea , Davoli Enrico , Franchi Carlotta , Diomede Luisa 
Published: March 2024

Abstract
Despite its high demand, the seafood processing industry grapples with a substantial challenge: generating significant waste, encompassing edible and inedible by-products. This leads to considerable financial losses and raises socioecological concerns regarding managing and disposing of such waste. Urgently addressing this issue, implementing sustainable seafood preservation methods becomes crucial. This study provides a comprehensive summary of chemical and physical approaches proposed in the literature to prevent seafood loss and diminish waste. A literature search from 2000 to January 2023 yielded 49 publications meeting the inclusion criteria, revealing a fragmented landscape of studies conducted on various fish products under diverse conditions. Chemical methods include organic acids and bio-derived preservatives; physical approaches comprise cold plasma, high hydrostatic pressure, and UV-C irradiation. The advantages and disadvantages of each technique were considered and discussed. Using chitosans, derived from natural sources and Generally Recognized As Safe, has emerged as the most promising approach. However, the discourse on fish preservation methods remains an evolving topic, weaving between the complexities of food safety, consumer satisfaction, and economic and environmental impacts. Further research is needed to investigate spoilage during production and critically evaluate the waste generated at different stages of the industrial process.
Comparing HEDIS performance of Dual Eligible Special Needs Plans with other coverage types for dually eligible people
Authors: Haviland Amelia M , Mathews Megan , Martino Steven C , Overton Yvette , Dembosky Jacob W , Maksut Jessica , Elliott Marc N 
Published: March 2024

Abstract
People eligible for both Medicare and Medicaid coverage (“dually eligible individuals”) have lower levels of income and assets and often higher health care needs and costs than those eligible for Medicare but not Medicaid coverage. Their 3 most common Medicare coverage options are Medicare Advantage (MA) Dual Eligible Special Needs Plans (D-SNPs), non–D-SNP MA plans, and fee-for-service (FFS) Medicare with a stand-alone prescription drug plan. No prior study has examined clinical quality of care for dually eligible individuals across these 3 coverage types. To fill that void, we used logistic regression to compare these coverage types on 6 HEDIS measures of clinical quality of care that were available for both MA and FFS (constructed from claims files). D-SNPs and non–D-SNP MA plans significantly outperformed FFS for all 6 measures for dually eligible individuals, by approximately 5 percentage points for 2 measures and by 18–34 percentage points for the other 4 measures. For the 4 measures with the greatest advantage over FFS, performance was 3–8 percentage points higher in D-SNPs than in non–D-SNP MA plans.
Chest wall tuberculosis: precision surgery with indigo carmine in limited cases for safe and intuitive lesion excision
Authors: Abe Sosei , Okabayashi Kan , Tokuishi Keita , Nakashima Hiroyasu , Maekawa Shin-Ichi , Hamatake Daisuke , Sato Toshihiko 
Published: March 2024

Abstract
In addition to treatment with antituberculosis drugs, complete surgical excision is important for the cure of chest wall tuberculosis. However, surgery is often challenging to perform due to different factors such as the strong adhesion of tuberculous lesions to the surrounding normal tissue, growth of neovascularization and presence of fragile necrotic tissues. Firm adhesions, bleeding and fragile tissue make it difficult to determine the boundary with normal tissue and completely excide the lesion. Moreover, ingenuity is required. Herein, we report the identification of the boundary between the lesion and normal tissue by injecting indigo carmine into the abscess to completely excide the lesion, which is considered an intuitive and safe method.
Affective Polarization Between Opinion-Based Groups in a Context of Low Partisan Discord: Measuring Its Prevalence and Consequences
Authors: Schieferdecker David , Joly Philippe , Faas Thorsten 
Published: March 2024

Abstract
Affective polarization has been primarily studied as a phenomenon between partisans. Recently, Hobolt, Leeper, & Tilley (2021) showed in the context of Brexit that affective polarization can also materialize around opinion-based groups, that is, political identities that form around extraordinary issues and cut through partisan lines. We generalize their findings by documenting the emergence of affective polarization around a new political conflict in a national context of low partisan discord. Specifically, we study divisions around the government response to the COVID-19 pandemic in Germany, a multiparty system where all established parties supported certain freedom restrictions to contain the pandemic. Drawing on numerous indicators and a survey experiment from a representative online panel study, we explored the (a) prevalence, (b) consequences, and (c) context of affective polarization between supporters and opponents of COVID-19 containment policies. We found that the majority of supporters and a minority of opponents showed indications of affective polarization. Affectively polarized citizens showed various group biases that make productive political and social interactions less likely. Affective polarization was stable over time and different from partisan and sociostructural conflicts. We raise questions for future research and discuss what it means for democracy when deep political rifts are not captured by main parties in a party system.
Genomics in nephrology: identifying informatics opportunities to improve diagnosis of genetic kidney disorders using a human-centered design approach
Authors: Romagnoli Katrina M , Salvati Zachary M , Johnson Darren K , Ramey Heather M , Chang Alexander R , Williams Marc S 
Published: March 2024

Abstract

Background
Genomic kidney conditions often have a long lag between onset of symptoms and diagnosis. To design a real time genetic diagnosis process that meets the needs of nephrologists, we need to understand the current state, barriers, and facilitators nephrologists and other clinicians who treat kidney conditions experience, and identify areas of opportunity for improvement and innovation.

Methods
Qualitative in-depth interviews were conducted with nephrologists and internists from 7 health systems. Rapid analysis identified themes in the interviews. These were used to develop service blueprints and process maps depicting the current state of genetic diagnosis of kidney disease.

Results
Themes from the interviews included the importance of trustworthy resources, guidance on how to order tests, and clarity on what to do with results. Barriers included lack of knowledge, lack of access, and complexity surrounding the case and disease. Facilitators included good user experience, straightforward diagnoses, and support from colleagues.

Discussion
The current state of diagnosis of kidney diseases with genetic etiology is suboptimal, with information gaps, complexity of genetic testing processes, and heterogeneity of disease impeding efficiency and leading to poor outcomes. This study highlights opportunities for improvement and innovation to address these barriers and empower nephrologists and other clinicians who treat kidney conditions to access and use real time genetic information.
Sir Charles Eastlake, the National Gallery and Milan
Authors: Avery-Quash Susanna 
Published: March 2024

Abstract
The first director of the National Gallery in London, Sir Charles Eastlake (1793–1865), was an Italophile. His favourite Italian city was Venice, where he once thought of buying a second home. The city he knew best, having lived there for fourteen years from 1816, was Rome. However, between 1854 and 1864, Eastlake made twenty-three trips to Milan, meaning that during his decade as director of the National Gallery from 1855, he visited Milan more often than any other place. This article suggests that Milan became a crucial hub for Eastlake, as the place where he connected with individuals who played important roles in several interconnected areas. Milan was significant, first, as an area rich in private collections, offering fertile ground for the discovery of potential acquisitions and a place from where it was relatively easy to export paintings because German-speaking Austria, which occupied north Italy and the Veneto until 1866, was comparatively lax about imposing export restrictions; second, as a place where art-historical knowledge and connoisseurship could be developed; and third, as a leading centre of picture conservation and framing.
Digital payments
Authors: Annan Francis , Cheung Chiman , Giné Xavier 
Published: March 2024

AbstractDespite the rapid growth in digital payments (DP) adoption and its positive socio-economic impacts in low-income countries, a large portion of the population remains disconnected from DP. At the same time, usage of DP conditional on adoption is low, highlighting the unexplored potential for financial inclusion and economic advancement. This paper reviews the burgeoning academic literature on DP and categorizes both macro-level adoption barriers (extensive margin) and micro-level usage challenges (intensive margin). We draw on the Transaction Cost Index, a new comprehensive database encompassing 16 low-income countries, to shed light on major themes in markets for DP. We conclude by outlining potential avenues for future research in this area.
Debottlenecking the L-DOPA 4,5-dioxygenase step with enhanced tyrosine supply boosts betalain production in Nicotiana benthamiana
Authors: Jung Soyoung , Maeda Hiroshi A 
Published: March 2024

Abstract
Synthetic biology provides emerging tools to produce valuable compounds in plant hosts as sustainable chemical production platforms. However, little is known about how supply and utilization of precursors is coordinated at the interface of plant primary and specialized metabolism, limiting our ability to efficiently produce high levels of target specialized metabolites in plants. L-Tyrosine is an aromatic amino acid precursor of diverse plant natural products including betalain pigments, which are used as the major natural food red colorants and more recently a visual marker for plant transformation. Here, we studied the impact of enhanced L-tyrosine supply on the production of betalain pigments by expressing arogenate dehydrogenase (TyrA) from table beet (Beta vulgaris, BvTyrAα), which has relaxed feedback inhibition by L-tyrosine. Unexpectedly, betalain levels were reduced when BvTyrAα was coexpressed with the betalain pathway genes in Nicotiana benthamiana leaves; L-tyrosine and 3,4-dihydroxy-L-phenylalanine (L-DOPA) levels were drastically elevated but not efficiently converted to betalains. An additional expression of L-DOPA 4,5-dioxygenase (DODA), but not CYP76AD1 or cyclo-DOPA 5-O-glucosyltransferase, together with BvTyrAα and the betalain pathway, drastically enhanced betalain production, indicating that DODA is a major rate-limiting step of betalain biosynthesis in this system. Learning from this initial test and further debottlenecking the DODA step maximized betalain yield to an equivalent or higher level than that in table beet. Our data suggest that balancing between enhanced supply (“push”) and effective utilization (“pull”) of precursor by alleviating a bottleneck step is critical in successful plant synthetic biology to produce high levels of target compounds.
Evaluation of a mobile COVID-19 vaccination van outreach service in an outer South London borough
Authors: Zelent Gina , Addison Kerry , King Emma , Moore James , Flowers Rachel 
Published: March 2024

Abstract

Background
This study aimed to explore differences in users of a COVID-19 mobile vaccine van service and users of a COVID-19 static vaccination hub, and the impact of changes in national COVID-19 vaccine policy on vaccine uptake.

Methods
The age distribution of male and female service users in each service was compared. The average number of vaccines administered per hour per week was analysed.

Results
Females aged 80–89 represented 51.9% (95% CI 49.5–54.3%) of female vaccine van users compared with 2.8% (95% CI 2.5–3.1%) of female static hub users. The static hub had significantly greater proportions of female service users in all other age brackets.
For males, the greatest difference was in those aged 70–79 who represented 29.8% (95% CI 27–32.6%) of vaccine van users and 16.6% (95% CI 16–17.2%) static hub users.
Fewer vaccines were administered 2–3 weeks before the COVID-19 autumn booster policy change compared with 2–3 weeks after; 1.92 versus 6.25 vaccines per hour, respectively (Mann–Whitney U = 7, n1 = 11, n2 = 8, P &lt; 0.01 two-tailed).

Conclusions
These findings suggest that a mobile vaccine van service is an effective model for increasing COVID-19 vaccination uptake in elderly residents, particularly after a national policy change.
Genome-wide identification of HMT gene family explores BpHMT2 enhancing selenium accumulation and tolerance in Broussonetia papyrifera
Authors: Chen Qiangwen , Zhu Changye , Guo Longfei , Bu Xianchen , Yang Wei , Cheng Shuiyuan , Cong Xin , Xu Feng 
Published: March 2024

Abstract
Broussonetia papyrifera, a valuable feed resource, is known for its fast growth, wide adaptability, high protein content and strong selenium enrichment capacity. Selenomethionine (SeMet), the main selenium form in selenium fortification B. papyrifera, is safe for animals and this enhances its nutritional value as a feed resource. However, the molecular mechanisms underlying SeMet synthesis remain unclear. This study identified three homocysteine S-methyltransferase genes from the B. papyrifera genome. The phylogenetic tree demonstrated that BpHMTs were divided into two classes, and BpHMT2 in the Class 2-D subfamily evolved earlier and possesses more fundamental functions. On the basis of the correlation between gene expression levels and selenium content, BpHMT2 was identified as a key candidate gene associated with selenium tolerance. Subcellular localization experiments confirmed the targeting of BpHMT2 in nucleus, cell membrane and chloroplasts. Moreover, three BpHMT2 overexpression Arabidopsis thaliana lines were confirmed to enhance plant selenium tolerance and SeMet accumulation. Overall, our finding provides insights into the molecular mechanisms of selenium metabolism in B. papyrifera, highlighting the potential role of BpHMT2 in SeMet synthesis. This research contributes to our understanding of selenium-enriched feed resources, with increased SeMet content contributing to the improved nutritional value of B. papyrifera as a feed resource.
Integrated small RNA, transcriptome and physiological approaches provide insight into Taxodium hybrid ‘Zhongshanshan’ roots in acclimation to prolonged flooding
Authors: Lu Yan , Zhang Shuqing , Xiang Peng , Yin Yunlong , Yu Chaoguang , Hua Jianfeng , Shi Qin , Chen Tingting , Zhou Zhidong , Yu Wanwen , Creech David L , Lu Zhiguo 
Published: March 2024

Abstract
Although Taxodium hybrid ‘Zhongshanshan’ 406 (Taxodium mucronatum Tenore × Taxodium distichum; Taxodium 406) is an extremely flooding-tolerant woody plant, the physiological and molecular mechanisms underlying acclimation of its roots to long-term flooding remain largely unknown. Thus, we exposed saplings of Taxodium 406 to either non-flooding (control) or flooding for 2 months. Flooding resulted in reduced root biomass, which is in line with lower concentrations of citrate, α-ketoglutaric acid, fumaric acid, malic acid and adenosine triphosphate (ATP) in Taxodium 406 roots. Flooding led to elevated activities of pyruvate decarboxylase, alcohol dehydrogenase and lactate dehydrogenase, which is consistent with higher lactate concentration in the roots of Taxodium 406. Flooding brought about stimulated activities of superoxide dismutase and catalase and elevated reduced glutathione (GSH) concentration and GSH/oxidized glutathione, which is in agreement with reduced concentrations of O2− and H2O2 in Taxodium 406 roots. The levels of starch, soluble protein, indole-3-acetic acid, gibberellin A4 and jasmonate were decreased, whereas the concentrations of glucose, total non-structural carbohydrates, most amino acids and 1-aminocyclopropane-1-carboxylate (ACC) were improved in the roots of flooding-treated Taxodium 406. Underlying these changes in growth and physiological characteristics, 12,420 mRNAs and 42 miRNAs were significantly differentially expressed, and 886 miRNA–mRNA pairs were identified in the roots of flooding-exposed Taxodium 406. For instance, 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8) was a target of Th-miR162-3p and 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4) was a target of Th-miR166i, and the downregulation of Th-miR162-3p and Th-miR166i results in the upregulation of ACS8 and ACO4, probably bringing about higher ACC content in flooding-treated roots. Overall, these results indicate that differentially expressed mRNA and miRNAs are involved in regulating tricarboxylic acid cycle, ATP production, fermentation, and metabolism of carbohydrates, amino acids and phytohormones, as well as reactive oxygen species detoxification of Taxodium 406 roots. These processes play pivotal roles in acclimation to flooding stress. These results will improve our understanding of the molecular and physiological bases underlying woody plant flooding acclimation and provide valuable insights into breeding-flooding tolerant trees.
MbEOMT1 regulates methyleugenol biosynthesis in Melaleuca bracteata F. Muell
Authors: Yang Chao , Lin Yongsheng , Xiang Xuwen , Shao Dandan , Qiu Ziwen , Li Yongyu , Wu Shaohua 
Published: March 2024

Abstract
Methyleugenol, a bioactive compound in the phenylpropene family, undergoes its final and crucial biosynthetic transformation when eugenol O-methyltransferase (EOMT) converts eugenol into methyleugenol. While Melaleuca bracteata F. Muell essential oil is particularly rich in methyleugenol, it contains only trace amounts of its precursor, eugenol. This suggests that the EOMT enzyme in M. bracteata is highly efficient, although it has not yet been characterized. In this study, we isolated and identified an EOMT gene from M. bracteata, termed MbEOMT1, which is primarily expressed in the flowers and leaves and is inducible by methyl jasmonate (MeJA). Subcellular localization of MbEOMT1 in the cytoplasm was detected. Through transient overexpression experiments, we found that MbEOMT1 significantly elevates the concentration of methyleugenol in M. bracteata leaves. Conversely, silencing of MbEOMT1 via virus-induced gene silencing led to a marked reduction in methyleugenol levels. Our in vitro enzymatic assays further confirmed that MbEOMT1 specifically catalyzes the methylation of eugenol. Collectively, these findings establish that the MbEOMT1 gene is critical for methyleugenol biosynthesis in M. bracteata. This study enriches the understanding of phenylpropene biosynthesis and suggests that MbEOMT1 could serve as a valuable catalyst for generating bioactive compounds in the future.
A new approach allows morphological recognition of cryptic diversity of the krill genus Hansarsia (formerly Nematoscelis)
Authors: Vereshchaka Alexander L , Shatravin Alexander V , Kulagin Dmitry N , Lunina Anastasiia A 
Published: March 2024

Abstract
Cryptic and pseudocryptic species are found on all major branches of the tree of life and probably represent a significant portion of undiscovered biodiversity, yet their identification is currently possible solely on the basis of molecular analyses. Here, we tested an alternative approach and hypothesized that all genetic clades might be identified on a morphological basis, and we analysed the morphology of nine previously detected genetic clades of Hansarsia. We made 169 sets of measurements (12 quantitative characters for each), ran multinomial logistic regression models, and showed a high ability of these models to generalize (i.e. to be able to classify correctly new specimens not included in our data matrix). The five to seven most ‘powerful’ characters provided a significant detection rate; these characters were shown to represent distinct evolutionary traits. Our findings cast some doubt on the presence of the ‘merely’ cryptic species; instead, we suggest that any genetic divergence found via traditional genetic markers is also mirrored in morphological divergence and can be detected using a combination of quantitative characters and appropriate mathematical tools. We provide a script key to genetic clades of the Atlantic Hansarsia based solely on morphological measurements and encourage other researchers to use quantitative morphological characters for detection of cryptic clades in other taxa.
Efficacy of Centers of Biomedical Research Excellence (CoBRE) grants to build research capacity in underrepresented states
Authors: Schaller Michael D. 
Published: March 2024

AbstractFederal funding for research has immediate and long‐term economic impact. Since federal research funding is regionally concentrated and not geographically distributed, the benefits are not fully realized in some regions of the country. The Established (previously Experimental) Program to Stimulate Competitive Research (EPSCoR) programs at several agencies, for example, the National Science Foundation, and the Institutional Development Award (IDeA) program at the National Institutes of Health were created to increase competitiveness for funding in states with historically low levels of federal funding. The Centers of Biomedical Research Excellence (CoBRE) award program is a component of the IDeA program. The CoBRE grants support research core facilities to develop research infrastructure. These grants also support the research projects of junior investigators, under the guidance of mentoring teams of senior investigators, to develop human resources at these institutions. Few studies have assessed the effectiveness of these programs. This study examines the investment and outcomes of the CoBRE grants from 2000 through 2022. The maturation of junior investigators into independently funded principal investigators is comparable to other mentoring programs supported by NIH. The investment in research cores resulted in substantial research productivity, measured by publications. Despite the successes of individual investigators and increased research infrastructure and productivity, the geographic distribution of federal and NIH research dollars has not changed. These results will be informative in consideration of policies designed to enhance the geographic distribution of federal research dollars.