bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative analysis of SEPN1 in glioma: Prognostic roles, functional implications, and potential therapeutic interventions
Authors: Wang Zisong , Wang Danwen , Wang Xuanyu , Xu Yihang , Yuan Yunhe , Chen Yuxin , Li Zhiqiang , Liu Xiaoping 
Published: February 2025

Background
SEPN1, a selenoprotein involved in redox regulation and endoplasmic reticulum stress response, has an unclear role in cancer. This study aims to investigate the expression, prognostic significance, and tumor microenvironment (TME) relevance of SEPN1 across pan-cancer, with a particular focus on glioma.

Methods
We analyzed SEPN1 expression and prognosis using the TCGA pan-cancer cohort. SEPN1 in glioma was further examined using data from TCGA, CGGA, GEO, and ZN-GC cohorts, along with survival analysis, single-cell RNA sequencing analysis, and enrichment analysis. We developed an SEPN1-related risk score (SRS) based on SEPN1-related long non-coding RNAs and validated its prognostic value. Drug sensitivity data and connectivity map analysis identified potential anti-glioma drugs based on the SRS.

Results
We found that SEPN1 was significantly upregulated in glioma, associated with poor prognosis, functioned as an independent risk factor, and predominantly expressed in malignant glioma cells. Enrichment analysis indicated the involvement of SEPN1 in immune-related processes and signaling pathways. Suppressing SEPN1 in glioblastoma cells inhibited proliferation and induced G2/M arrest and apoptosis. The SRS demonstrated strong prognostic value and correlated with enhanced immune infiltration in the glioma TME. Potential anti-glioma drugs were identified based on the SRS.

Conclusions
SEPN1 emerges as a novel biomarker and therapeutic target in glioma, providing a basis for future development of targeted therapies.
Composition, determinants, and risk factors of low birth weight in Sri Lanka
Authors: Mettananda Sachith , Herath Himali , Thewage Ayesha , Nanayakkara Kumudu , Liyanage Indeewari , Udani K. S. , Savanadasa Rajika , Goonewardena Sampatha , Gamhewage Nimesha , Hewamalage Asiri , Rowel Dhammica , Daniel Abner Elkan , de Silva Chithramalee , Perera Susie 
Published: February 2025

Introduction
Low birth weight continues to pose significant challenges to healthcare systems worldwide. Despite substantial improvement in various public health indicators, many developing countries have failed to achieve a significant reduction in low birth weight rates. One major obstacle is the sparsity of data on the determinants of low birth weight. Here, we aim to determine the composition and risk factors for low birth weight in Sri Lanka, a prototype developing nation.

Methodology
We conducted a countrywide multicentre cross-sectional study in August and September 2023 in 13 purposively selected hospitals representing all nine provinces and different tiers of specialist hospitals in Sri Lanka. All live-born neonates were recruited prospectively, and their mothers were interviewed by trained data collectors to gather information on socio-demographic background, medical and obstetric history, and delivery details. Birth weight was measured immediately after the birth by trained healthcare personnel attending the delivery.

Results
A total of 9130 live-born neonates were recruited, of which 52% were males. The mean birth weight was 2827g (95%CI 2817-2838g), and 1865 (20.4%) newborns were low birth weight. The prevalence of prematurity was 10.9% (n = 998), and 1819 (20.0%) neonates were born small for gestational age. Of the low birth weight neonates, 64% were small for gestational age, and 37% were preterm; 11% were both small for gestational age and preterm. Teenage pregnancy (p = 0.022), low maternal pre-pregnancy body mass index (p<0.001), inadequate weight gain during pregnancy (p<0.001), maternal anaemia at delivery (p = 0.020), chronic lung disease (p = 0.019), and pregnancy induced hypertension (p = 0.019) were significant modifiable risk factors for small for gestational age.

Conclusion
This study presents the results of one of the most extensive country-wide studies evaluating the composition and determinants of low birth weight. The study highlights the importance of considering small for gestational age and prematurity as separate categories of low birth weight. Small for gestational age contributes to approximately two-thirds of the low birth weight burden. Therefore, targeting modifiable risk factors for small for gestational age while mitigating the burden of prematurity is the most feasible approach to reduce the prevalence of low birth weight in developing countries, including Sri Lanka.
Aortoduodenal Syndrome: A Rare Case of Duodenal Obstruction Due to Endovascular Aneurysm Endoleak
Authors: Adhikari Raksha , Patel Parth , Tait Christopher 
Published: February 2025

ABSTRACT
Aortoduodenal syndrome results from expansion of an abdominal aortic aneurysm, leading to symptoms of upper gastrointestinal obstruction. Endovascular aneurysm repair of large or symptomatic aortic aneurysms has become an important treatment modality for patients with abdominal aortic aneurysm. However, a large proportion of these grafts are complicated by endoleaks that can lead to a spectrum of significant symptoms. We present a case of rapidly worsening gastrointestinal obstructive symptoms in a patient with an already repaired aortic stent graft and a solitary kidney, found to be due to a type III endoleak that improved after emergent repeat endovascular aneurysm repair.
Correlation of radiographic and histopathological changes with IL-17 and advanced oxidation protein products in knee osteoarthritic individuals with metabolic syndrome
Authors: Awan Uzma Naseer , Waraich Rizwana Sanaullah , Noor Syed Shahid , Siddiqui Iftikhar Ahmed , Nangrejo Ruqaya 
Published: February 2025

Abstract

Objectives
Recent studies show that osteoarthritis and metabolic syndrome (MetS) represent significant global health concerns, sharing common pathological processes involving inflammation and oxidative stress. The study aimed to compare the radiological and histological severity of osteoarthritis in patients with and without MetS and further correlates them with oxidative stress and inflammatory markers in serum and synovial fluid (SF). Hypothesis: The study hypothesized that IL-17 and advanced oxidation protein products (AOPPs) are correlated with OA severity and progression in MetS patients.

Methods
This cross-sectional study included 78 patients of advanced knee osteoarthritis, 40 with MetS and 38 without, matched for age. Clinical history and anthropometric measurements were recorded, and presurgical knee X-rays were evaluated using the Kellgren–Lawrence system. Histological grading of hematoxylin & eosin stained infrapatellar fat pad (IFP) and cartilage sections was performed. AOPPs and Interleukin-17 levels were measured in serum and SF, employing sandwich enzyme-linked immunosorbent assay.

Results
In the MetS group, the severity of osteoarthritis was higher compared to non-MetS group, as evidenced by histological evaluation of the articular cartilage and IFP (p<0.05). The histological grading of IFP demonstrated positive correlation (p<0.05) with histological cartilage grade. Additionally, it exhibited a positive correlation with interleukin-17 and AOPPs in both SF and serum (p<0.05). While histological cartilage grade showed a positive correlation with AOPPs concentration in the serum and SF (p<0.05).

Conclusions
MetS accelerates osteoarthritis progression, and positive correlation between molecular markers and histological severity suggests the contribution of inflammation and oxidative stress in the disease’s pathogenesis.
Tabú da democracia?! Solidariedade multiétnica e luta pelo poder
Authors: Sambé Jeraldino António , Carvalho Ricardo Ossagô de 
Published: February 2025

Este artigo objetiva-se analisar como a solidariedade multiétnica e sua articulação política articulada dentro do contexto da arena do poder na Guiné-Bissau, buscando compreender as dinâmicas e tensões em volta da identidade coletiva fase as ameaças da etnicidade, clientelismo, politização, e luta pela manutenção dos recursos políticos e sua legitimação como regra do jogo. Em relação à metodologia, foi utilizada a revisão da literatura e abordagem qualitativa desse fenômeno a partir de estudos nacionais e internacionais. O desenrolar desse mecanismo político numa sociedade fragilizada como a bissau-guineense, tem impactado a configuração democrática, abrindo assim as portas para instabilidade governativa e política de alianças clientelistas e transformando-se nas ferramentas inevitáveis que subsidiam toda a dinâmica política contemporânea guineense. Nesta conjuntura, a Guiné-Bissau enquanto país com uma rica diversidade étnica formada pelas complexidades socioculturais e históricas, paradoxalmente as articulações da etnicidade no campo político e das relações de poder tem movido a política nos últimos anos, como demonstra algumas literaturas, estes procedimentos da etnicidade, politização e clientelismo comprometem arena democrático guineense.
Caracterización y capacidad de adaptación climática de ganaderos en dos regiones de Costa Rica
Authors: Cortés-Muñoz Gerardo , Fuentes-Navarro Eduardo , Mercado-Curi Waldemar 
Published: February 2025

Introducción. Las lecherías en Costa Rica han sido clasificadas en tres sistemas de producción: los sistemas especializados de altura, los especializados de bajura y los de doble propósito. No obstante, esta tipificación no muestra su nivel de complejidad actual, en donde se encuentra una amplia variedad de sistemas productivos con base en el manejo de prácticas sanitarias, reproductivas y de alimentación. Objetivo. Realizar una caracterización de los sistemas de producción de leche en dos regiones ganaderas representativas y sus capacidades en la adaptación tecnológica al cambio climático. Materiales y métodos. Para la determinación de las características técnico-productiva, social y económica de las fincas ubicadas en las regiones Chorotega y Huetar Norte, se llevó a cabo la aplicación de una encuesta diseñada y validada. La información fue recolectada entre abril y mayo del 2021 y se entrevistaron 143 finqueros, lo que permitió evaluar un total de 193 variables. Seguidamente, se realizó un Análisis de Conglomerados (AC), y se ejecutó el análisis de conglomerados jerárquicos mediante el método de Ward. Resultados. Se obtuvieron tres conglomerados, con capacidades de gestión de finca; alta, media y baja relacionadas con sus capacidades de adaptación al cambio climático. El primer grupo se conformó por productores con un nivel superior de educación y de gestión del predio (alta capacidad), reflejado así en la adopción de más estrategias de adaptación. Las fincas del segundo grupo (mediana capacidad), con un manejo tecnificado inferior al primer grupo, y el tercer grupo de fincas (baja capacidad), que son productores con el nivel educativo más bajo, demostraron una capacidad de gestión tecnológica más limitada. Conclusión. La actividad ganadera se caracterizó por tener tres grupos de productores con diferentes capacidades de adaptación al cambio climático.
Detecting Tidal Periodicities of Earthquake Occurrence in Japan Using Spectral Analyses
Authors: Zheng Jinbo , Wang Wei , Zhang Jinhai 
Published: February 2025

Abstract
The periodicities of earthquake occurrences are critical for understanding the mechanism of earthquake nucleation and triggering, yet it remains a topic of ongoing discussion. Here, we develop an annual spectral averaging method to extract potential periodicities from the Japan Meteorological Agency catalog. Unlike previous methods, such as Schuster’s test or Schuster spectrum methods, our method neither relies on the tidal phase information nor requires prior knowledge of assumed periodicities. We simultaneously identified two tidal periodicities, 12.4 and 24 hr, which are consistent with the semidiurnal lunar tide and diurnal solar tide, respectively. This is the first time that the short periodicity smaller than 24 hr was detected by the spectral analysis method. The presence of 12.4 hr periodicity provides the first compelling evidence for tidal triggering of earthquakes. This study may provide a new view for further exploration into external triggering mechanisms of seismicity.
Monitoring Submarine Volcanic Activities in the Izu–Bonin–Mariana Arc Using the International Monitoring System Hydrophone Array Signals
Authors: Tanaka Satoru , Nakano Masaru , Nakajima Tomoya , Yoshimitsu Junko , Obayashi Masayuki , Metz Dirk 
Published: February 2025

Abstract
International Monitoring System hydrophone arrays are powerful tools for monitoring submarine volcanic activity. In this study, we routinely analyzed the signals of the triplet H11S, located off the coast of Wake Island, beginning in July 2022, to monitor submarine volcanic activity, particularly in the Izu–Bonin–Mariana arc. We removed the instrument response and applied a band-pass filter with cutoff frequencies of 4 and 8 Hz. Subsequently, the hydrophone signals were divided into 10 s time windows without overlapping. Semblance analysis was performed to determine the incoming direction and apparent velocity. The maximum obtained semblance values typically ranged from 0.5 to 0.9, and only values ≥0.7 were interpreted as significant. In our observation period (July 2022 to August 2024), we detected significant hydroacoustic signals from six sites (Ahyi, Daikoku, Kaitoku, Sofugan, Sumisu-jima, and Hachijo-jima) and compared them with those obtained from the past eruptions at Fukutoku-Oka-no-Ba. We discussed the characteristics of individual waveforms and spectrograms of some detected signals and found that frequency index values, defined as the ratio of mean spectral amplitudes between higher and lower frequency components, could be helpful in objectively discriminating the origins of hydroacoustic signals.
La pisciculture artisanale du <em>Tilapia</em> en Afrique : Analyse de différents systèmes d'élevage et de leur niveau de développementge et de leur niveau de développement
Authors: Lazard Jérome , Morissens Pierre , Parrel P. 
Published: February 2025

Le continent africain n'a pas de tradition piscicole et de nombreuses tentatives ont été menées au cours des 40 dernières années pour développer cette activité, principalement basée sur la culture du Tilapia. Aujourd'hui, seuls quelques projets de développement ont réussi à sensibiliser les populations rurales africaines à cette nouvelle activité, et la plupart d'entre eux ont été ou sont encore menés dans le cadre des administrations locales. De plus, les fermes piscicoles privées gérées par des Africains sont encore très peu nombreuses sur le continent. La seule aquaculture existante en milieu rural est d'un niveau technique très faible, entraînant des revenus financiers médiocres pour le paysan qui, en général, retourne tôt ou tard à son activité agricole traditionnelle. Cet article décrit plusieurs programmes de développement de l'aquaculture du Tilapia basés sur 3 techniques d'élevage artisanales différentes liées à 3 conditions socio-économiques et environnementales très différentes : étangs inclus dans un aménagement hydro-agricole (Côte d'Ivoire), cages flottantes dans une rivière sahélienne (Niger) et enclos dans une lagune saumâtre (Bénin). Pour chaque système de pisciculture, les données suivantes sont comparées :- la description et le coût de la structure d'élevage, ainsi que les conditions et contraintes de sa mise en œuvre ;- principaux résultats sur les différentes étapes de la production de Tilapia ;- aspects économiques : coûts de production, bénéfices et situation du marché ;- le niveau de maîtrise des techniques décrites et le niveau de vulgarisation auprès de la population rurale ou des pisciculteurs.À partir de l'étude de ces trois situations, des conclusions sont formulées et des recommandations sont suggérées pour l'avenir du développement de l'aquaculture du tilapia sur le continent africain.
A modular encapsulation system for precision delivery of proteins, nucleic acids and therapeutics
Authors: Parton Robert , Luong Thai Duong , Martel Nick , Rae James , Lo Harriet , Lim Ye-Wheen , Wu Yeping , McMahon Kerrie-Ann , Fletcher Nick , Thurecht Kristofer , Johnston Angus , Ariotti Nicholas , Hall Thomas 
Published: February 2025

Abstract

Targeted nanoparticles have the potential to revolutionize therapeutics for medical applications. Here, we demonstrate the utility of a flexible precision nanovesicle delivery system for functional delivery of DNA, RNA, proteins and drugs into target cells. Nanovesicles generated by the membrane sculpting protein caveolin, termed caveospheres, can be loaded with RNA, DNA, proteins or drugs post-synthesis or incorporate genetically-encoded cargo proteins during production without the need for protein purification. Functionalized fluorescently-labeled caveospheres form a modular system that shows high stability in biological fluids, specific uptake by target-positive cells, and can deliver proteins, drugs, DNA, and mRNA directly to the cytoplasm and nuclei of only the target cells. We demonstrate their application as a targeted transfection system for cells in culture, as a system to study endosomal escape, and critically, their efficacy in precision tumor killing in vivo.
Anyonization of bosons
Authors: Nägerl Hanns-Christoph , Dhar Sudipta , Wang Botao , Horvath Milena , Vashisht Amit , Zeng Yi , Zvonarev Mikhail , Goldman Nathan , Guo Yanliang , Landini Manuele 
Published: February 2025

Abstract

Anyons are low-dimensional quasiparticles that obey fractional statistics, hence interpolating between bosons and fermions. In two dimensions, they exist as elementary excitations of fractional
quantum Hall states and they are believed to enable topological quantum computing. One-dimensional (1D) anyons have been theoretically proposed, but their experimental realization has proven to be difficult. Here, we observe emergent anyonic correlations in a 1D strongly-interacting quantum gas, resulting from the phenomenon of spin-charge separation. A mobile impurity provides the necessary spin degree of freedom to engineer anyonic correlations in the charge sector and simultaneously acts as a probe to reveal these correlations. Starting with bosons, we tune the statistical phase to transmute bosons via anyons to fermions and observe an asymmetric momentum distribution, hallmark of anyonic correlations. Going beyond equilibrium conditions, we observe dynamical fermionization of the anyons. Our work opens up the door to the exploration of nonequilibrium anyonic phenomena in a highly controllable setting.
Rhaponticin inhibits the proliferation, migration, and invasion of head and neck squamous cell carcinoma (HNSCC) cells through modulation of the IL6/STAT3 signaling pathway
Authors: Wei Hongcheng , Wang Shanshan , Wan Jiayue , Li Sicheng , Wang Wei , Zhu Jiajun , Jiang Lin , Shao Yisen , Wu Yuan 
Published: February 2025

Abstract

Background: Rhaponticin, a bioactive compound derived from rhubarb, has been demonstrated anti-tumor effects in various types of cancer. However, its impact on head and neck squamous cell carcinoma (HNSCC) remains unexplored. This study aims to investigate the specific molecular mechanisms by which Rhaponticin inhibits the invasion and metastasis of HNSCC cells.
Method: The potential target genes that rhaponticin acts on in HNSCC were identified using online databases. The mechanisms by which rhaponticin influences the occurrence and progression of HNSCC were investigated through network pharmacology, molecular docking, bioinformatics analysis, and cellular experiments.
Result: Using network pharmacology, we identified 40 hub genes from the collected gene set. Subsequently, by analyzing The Cancer Genome Atlas (TCGA) data with four machine learning algorithms, we identified IL-6 as a potential target associated with the occurrence and progression of head and neck squamous cell carcinoma (HNSCC). Based on the average expression level of IL-6, we classified the samples into high-expression and low-expression groups and conducted survival analysis. Our results indicated that IL-6 expression was significantly correlated with patient survival. Gene Set Enrichment Analysis (GSEA) revealed that Rhaponticin might influence HNSCC via the IL6/STAT3 signaling pathway. Using the CIBERSORT algorithm, we assessed the differences in infiltration levels of 22 immune cell types between the high and low IL-6 expression groups. Our findings suggest that multiple immune cells may be involved in the pathogenesis of HNSCC. Additionally, we analyzed single-cell RNA sequencing (scRNA-seq) data from the GEO database to compare IL6 expression levels in tumor and normal tissues and evaluated its prognostic impact using Receiver Operating Characteristic (ROC) curve analysis. Molecular docking studies demonstrated that Rhaponticin binds stably to IL6. In the experimental section, we used two HNSCC cell lines (CAL27 and SCC9) to investigate the effects of Rhaponticin. Our results showed that Rhaponticin effectively inhibited cell proliferation, invasion, and migration, and reduced the expression of proteins in the IL6/STAT3 signaling pathway.
Conclusion: Rhaponticin shows promise in treating HNSCC by inhibiting the IL6/STAT3 signaling pathway.
Efficacy of a New Selective Indole-based Histone Deacetylase 10 Inhibitor in Targeted Anticancer Therapy
Authors: Tarawneh Amer H. , Al-Trawneh Salah A. , Yesiloglu Talha Z. , Zessin Matthes , Robaa Dina , Barinka Cyril , Schutkowski Mike , Sippl Wolfgang , Ross Samir A. 
Published: February 2025

Abstract

Histone deacetylase (HDAC) inhibitors represent a newer class of anti-cancer agents that play a key role in both epigenetic and non-epigenetic regulation, leading to cancer cell death, apoptosis, and cell cycle arrest.. These inhibitors are being tested in numerous clinical trials against various diseases, including both hematological and solid malignancies. In the present study, we synthesized novel bicyclic hydroxamic acid derivatives and tested them in vitro against class I and IIb HDACs to investigate their inhibitory activity and selectivity. We demonstrate that compound 6 inhibits HDAC10 with high specificity over HDAC6, with no significant impact on class I HDACs. Compound 1 shows the best inhibitory activity against HDAC10, with IC50 0.41 ± 0.02 nM. Compound 4 revealed a preference toward HDAC6, with an IC50 value of 2.5 ± 0.3 nM. Compounds 2 and 3 demonstrated high selectivity toward class IIb over class I HDACs. Docking and molecular dynamics studies revealed that compound 1 fits well into the active site of HDAC10, forming stable and strong interactions with key residues F204, D94, W205, and E274 in HDAC10. In addition, we tested these compounds against a panel of four human solid tumor cell lines. Furthermore, non-cancerous kidney cell lines (LLC-PK1 and VERO) were employed to determine the anti-cell proliferative activity of these compounds toward noncancerous cells.