bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing
Authors: Tamada Atsushi , Kumada Tatsuro , Zhu Yan , Matsumoto Tomoko , Hatanaka Yumiko , Muguruma Keiko , Chen Zhe , Tanabe Yasuto , Torigoe Makio , Yamauchi Kenta , Oyama Hiroshi , Nishida Kazuhiko , Murakami Fujio 
Published: November 2008

Abstract
Background
Robo1, Robo2 and Rig-1 (Robo3), members of the Robo protein family, are candidate receptors for the chemorepellents Slit and are known to play a crucial role in commissural axon guidance in the spinal cord. However, their roles at other axial levels remain unknown. Here we examine expression of Robo proteins by cerebellofugal (CF) commissural axons in the rostral hindbrain and investigate their roles in CF axon pathfinding by analysing Robo knockout mice.

Results
We analysed the expression of Robo proteins by CF axons originating from deep cerebellar neurons in rodent embryos, focusing on developmental stages of their midline crossing and post-crossing navigation. At the stage of CF axon midline crossing, mRNAs of Robo1 and Robo2 are expressed in the nuclear transitory zone of the cerebellum, where the primordium of the deep cerebellar nuclei are located, supporting the notion that CF axons express Robo1 and Robo2. Indeed, immunohistochemical analysis of CF axons labelled by electroporation to deep cerebellar nuclei neurons indicates that Robo1 protein, and possibly also Robo2 protein, is expressed by CF axons crossing the midline. However, weak or no expression of these proteins is found on the longitudinal portion of CF axons. In Robo1/2 double knockout mice, many CF axons reach the midline but fail to exit it. We find that CF axons express Rig-1 (Robo3) before they reach the midline but not after the longitudinal turn. Consistent with this in vivo observation, axons elicited from a cerebellar explant in co-culture with a floor plate explant express Rig-1. In Rig-1 deficient mouse embryos, CF axons appear to project ipsilaterally without reaching the midline.

Conclusion
These results indicate that Robo1, Robo2 or both are required for midline exit of CF axons. In contrast, Rig-1 is required for their approach to the midline. However, post-crossing up-regulation of these proteins, which plays an important role in spinal commissural axon guidance, does not appear to be required for the longitudinal navigation of CF axons after midline crossing. Our results illustrate that although common mechanisms operate for midline crossing at different axial levels, significant variation exists in post-crossing navigation.
The ventro-medial prefrontal cortex: a major link between the autonomic nervous system, regulation of emotion, and stress reactivity?
Authors: Hänsel Alexander , von Känel Roland 
Published: November 2008

Abstract
Recent progress in neuroscience revealed diverse regions of the CNS which moderate autonomic and affective responses. The ventro-medial prefrontal cortex (vmPFC) plays a key role in these regulations. There is evidence that vmPFC activity is associated with cardiovascular changes during a motor task that are mediated by parasympathetic activity. Moreover, vmPFC activity makes important contributions to regulations of affective and stressful situations.
This review selectively summarizes literature in which vmPFC activation was studied in healthy subjects as well as in patients with affective disorders. The reviewed literature suggests that vmPFC activity plays a pivotal role in biopsychosocial processes of disease. Activity in the vmPFC might link affective disorders, stressful environmental conditions, and immune function.
CBP/p300-Interacting Transactivator, with Glu/Asp-Rich C-Terminal Domain, 2, and Pre-B-Cell Leukemia Transcription Factor 1 in Human Adrenal Development and Disease
Authors: Ferraz-de-Souza Bruno , Martin Franziska , Mallet Delphine , Hudson-Davies Rebecca E. , Cogram Patricia , Lin Lin , Gerrelli Dianne , Beuschlein Felix , Morel Yves , Huebner Angela , Achermann John C. 
Published: November 2008

AbstractContext: Disorders of adrenal development result in significant morbidity and mortality. However, the molecular basis of human adrenal development, and many forms of disease, is still poorly understood.Objectives: We evaluated the role of two new candidate genes, CBP/p300-interacting transactivator, with Glu/Asp-rich C-terminal domain, 2 (CITED2), and pre-B-cell leukemia transcription factor 1 (PBX1), in human adrenal development and disease.Design: CITED2 and PBX1 expression in early human fetal adrenal development was assessed using RT-PCR and in situ hybridization. The regulation of CITED2 and PBX1 by steroidogenic factor-1 (SF-1) and dosage-sensitive sex reversal, adrenal hypoplasia congenital, critical region on the X chromosome, gene-1 (DAX1) was evaluated in NCI-H295R human adrenocortical tumor cells by studying promoter regulation. Finally, mutational analysis of CITED2 and PBX1 was performed in patients with primary adrenal disorders.Results: CITED2 and PBX1 are expressed in the human fetal adrenal gland during early development. Both genes are activated by SF-1 in a dose-dependent manner in NCI-H295R cells, and, surprisingly, PBX1 is synergistically activated by SF-1 and DAX1. Mutational analysis failed to reveal significant coding sequence changes in individuals with primary adrenal disorders.Conclusions: CITED2 and PBX1 are likely to be important mediators of adrenal development and function in humans, but mutations in these genes are not common causes of adrenal failure in patients in whom a molecular diagnosis remains unknown. The positive interaction between DAX1 and SF-1 in regulating PBX1 may be an important mechanism in this process.
Lifestyle and Pharmacological Approaches to Weight Loss: Efficacy and Safety
Authors: Bray George A. 
Published: November 2008

ABSTRACTContextObesity results from a prolonged small positive energy balance, and its treatment needs to reverse this imbalance.Evidence AcquisitionCitations retrieved from PubMed and The Handbook of Obesity 2008 were selected to illustrate the points.Evidence SynthesisMany different diets have been tried to treat obesity, and weight loss occurs with all of them. There is currently no evidence that clearly supports a superiority of one macronutrient composition for diets used for weight loss. The principal effect seems to be the degree of adherence to the prescribed calorie reduction. Lifestyle strategies to modify eating behavior can be used in individual counseling sessions or in groups, both of which are important in helping patients modify their patterns of eating. Physical activity is particularly important in helping patients maintain a weight loss once achieved and is less valuable for weight loss itself. Food intake is controlled through many different mechanisms, but only a few drugs have been developed that tap these mechanisms. Orlistat, which blocks intestinal lipase, is one; sibutramine, a serotonin-norepinephrine reuptake inhibitor, is a second. Surgical approaches provide the most dramatic weight loss and have been demonstrated to reduce long-term mortality and reduce the incidence of diabetes.ConclusionsWeight loss can be achieved by many methods, but the surgical procedures appear to be the most durable.
The monitoring of the turning tool wear process using an artificial neural network. Part 1: The experimental set-up and experimental results
Authors: Balan G C , Epureanu A 
Published: November 2008

The study of machine tool dynamics is performed here as ‘monitoring’, which involves the checking and improving of machine functioning. A computer processes signals collected from certain sensors. These data then lead to the monitoring decision, which is to associate the current state of operation with one of the classes from a set of known classes. For monitoring in turning, the classes (tool conditions) are shown. The paper by Du, Elbestawi, and Wu (1995) represents the main source of inspiration for this paper, the present contribution being the improvement of the classes and of the monitoring indices. It is shown that the strains produced by Fy can be neglected and only the main cutting force is measured by means of the tensometer system. To validate the recordings, couples of recordings having working parameters quite similar in value were selected; nonetheless, these couples belonged to different classes. It is observable that for the recordings whose cutting working conditions are similar, the average value of force Fz increases as the cutting depth and wear increase. The experimental set-up and experimental results are presented.
Control chart pattern clustering using a new self-organizing spiking neural network
Authors: Pham D T , Packianather M S , Charles E Y A 
Published: November 2008

This paper focuses on the architecture and learning algorithm associated with using a new self-organizing delay adaptation spiking neural network model for clustering control chart patterns. This temporal coding spiking neural network model employs a Hebbian-based rule to shift the connection delays instead of the previous approaches of delay selection. Here the tuned delays compensate the differences in the input firing times of temporal patterns and enables them to coincide. The coincidence detection capability of the spiking neuron has been utilized for pattern clustering. The structure of the network is similar to that of a Kohonen self-organizing map (SOM) except that the output layer neurons are coincidence detecting spiking neurons. An input pattern is represented by the neuron that is the first to fire among all the competing spiking neurons. Clusters within the input data are identified with the location of the winning neurons and their firing times. The proposed self-organized delay adaptation spiking neural network (SODA_SNN) has been utilized to cluster control chart patterns. The trained network obtained an average clustering accuracy of 96.1 per cent on previously unseen test data. This was achieved with a network of 8 × 8 spiking neurons trained for 20 epochs containing 1000 training examples. The improvement in clustering accuracy achieved by the proposed SODA_SNN on the unseen test data was twice as much as that on the training data when compared to the SOM.
Cobalt-catalyzed Cross-coupling Reactions of Aryl Bromides with Alkyl Grignard Reagents
Authors: Hamaguchi Hiroyuki , Uemura Minoru , Yasui Hiroto , Yorimitsu Hideki , Oshima Koichiro 
Published: November 2008

Abstract
Aryl bromides react with primary alkyl Grignard reagents in the presence of N,N,N′,N′-tetramethyl-1,3-propanediamine and catalytic amounts of cobalt(II) chloride and an N-heterocyclic carbene to yield the corresponding cross-coupling products in high yields.
Highly Efficient Enantioresolution of 2,2-Dimethyl-3-hexanol with Cholamide Crystals on the Basis of Intercalation and Bilayer Inversion
Authors: Aburaya Kazuaki , Murai Taketoshi , Hisaki Ichiro , Tohnai Norimitsu , Miyata Mikiji 
Published: November 2008

Abstract
Enantioresolution of racemic 2,2-dimethyl-3-hexanol in solid state was achieved with four kinds of crystalline matrices of cholamide, yielding its S enantiomer in high purity and yields. This intercalation method accompanied bilayer inversion on their lipophilic sides.
Computer-assisted osteotomy of the lateral femoral condyle with non-constrained total knee replacement in severe valgus knees
Authors: Hadjicostas P. T. , Soucacos P. N. , Thielemann F. W. 
Published: November 2008

We describe the mid-term results of a prospective study of total knee replacement in severe valgus knees using an osteotomy of the lateral femoral condyle and computer navigation. There were 15 knees with a mean valgus deformity of 21° (17° to 27°) and a mean follow-up of 28 months (24 to 60). A cemented, non-constrained fixed bearing, posterior-cruciate-retaining knee prosthesis of the same design was used in all cases (Columbus-B. Braun; Aesculap, Tuttlingen, Germany). All the knees were corrected to a mean of 0.5° of valgus (0° to 2°). Flexion of the knee had been limited to a mean of 85° (75° to 110°) pre-operatively and improved to a mean of 105° (90° to 130°) after operation. The mean Knee Society score improved from 37 (30 to 44) to 90 points (86 to 94). Osteotomy of the lateral femoral condyle combined with computer-assisted surgery gave an excellent mid-term outcome in patients undergoing total knee replacement in the presence of severe valgus deformity.
Diabetes and smoking as prognostic factors after cervical laminoplasty
Authors: Kim H.-J. , Moon S.-H. , Kim H.-S. , Moon E.-S. , Chun H.-J. , Jung M. , Lee H.-M. 
Published: November 2008

We reviewed 87 patients who had undergone expansive cervical laminoplasty between 1999 and 2005. These were divided into two groups: those who had diabetes mellitus and those who did not. There were 31 patients in the diabetes group and 56 in the control group. Although a significant improvement in the Japanese Orthopaedic Association score was seen in both groups, the post-operative recovery rate in the control group was better than that of the diabetic group. The patients’ age and symptom duration adversely affected the rate of recovery in the diabetic group only. Smoking did not affect the outcome in either group. A logistic regression analysis found diabetes and signal changes in the spinal cord on MRI to be significant risk factors for a poor outcome (odds ratio 2.86, 3.02, respectively). Furthermore, the interaction of diabetes with smoking and/or age increased this risk. We conclude that diabetes mellitus, or the interaction of this with old age, can adversely affect outcome after cervical laminoplasty. However, smoking alone cannot be regarded as a risk factor.
Primary open reduction and fixation compared with delayed corrective arthrodesis in the treatment of tarsometatarsal (Lisfranc) fracture dislocation
Authors: Rammelt S. , Schneiders W. , Schikore H. , Holch M. , Heineck J. , Zwipp H. 
Published: November 2008

Fracture-dislocations of the tarsometatarsal (Lisfranc) joints are frequently overlooked or misdiagnosed at initial presentation. This is a comparative cohort study over a period of five years comparing primary open reduction and internal fixation in 22 patients (23 feet) with secondary corrective arthrodesis in 22 patients (22 feet) who presented with painful malunion at a mean of 22 months (1.5 to 45) after injury. In the first group primary treatment by open reduction and internal fixation for eight weeks with Kirschner-wires or screws was undertaken, in the second group treatment was by secondary corrective arthrodesis. There was one deep infection in the first group. In the delayed group there was one complete and one partial nonunion. In each group 20 patients were available for follow-up at a mean of 36 months (24 to 89) after operation. The mean American Orthopaedic Foot and Ankle Society midfoot score was 81.4 (62 to 100) after primary treatment and 71.8 (35 to 88) after corrective arthrodesis (t-test; p = 0.031). We conclude that primary treatment by open reduction and internal fixation of tarsometatarsal fracture-dislocations leads to improved functional results, earlier return to work and greater patient satisfaction than secondary corrective arthrodesis, which remains a useful salvage procedure providing significant relief of pain and improvement in function.
Methicillin-resistant Staphylococcus aureus in orthopaedic surgery
Authors: Patel A. , Calfee R. P. , Plante M. , Fischer S. A. , Arcand N. , Born C. 
Published: November 2008

Methicillin-resistant Staphylococcus aureus (MRSA) has become a ubiquitous bacterium in both the hospital and community setting. There are two major subclassifications of MRSA, community-acquired and healthcare-acquired, each with differing pathogenicity and management. MRSA is increasingly responsible for infections in otherwise healthy, active adults. Local outbreaks affect both professional and amateur athletes and there is increasing public awareness of the issue. Health-acquired MRSA has major cost and outcome implications for patients and hospitals. The increasing prevalence and severity of MRSA means that the orthopaedic community should have a basic knowledge of the bacterium, its presentation and options for treatment. This paper examines the evolution of MRSA, analyses the spectrum of diseases produced by this bacterium and presents current prevention and treatment strategies for orthopaedic infections from MRSA.
Revision arthroscopic capsulolabral reconstruction for recurrent instability of the shoulder
Authors: Patel R. V. , Apostle K. , Leith J. M. , Regan W. D. 
Published: November 2008

We have investigated the outcome of arthroscopic revision surgery for recurrent instability of the shoulder after failed primary anterior stabilisation. We identified 40 patients with failed primary open or arthroscopic anterior stabilisation of the shoulder who had been treated by revision arthroscopic capsulolabral reconstruction and followed up for a mean of 36 months (12 to 87). There were 34 men and six women with a mean age of 33.1 years (15 to 48). Details of the patients, the technique of the primary procedure, the operative findings at revision and the clinical outcome were evaluated by reviewing the medical records, physical examination and the use of the Western Ontario shoulder instability index score, the American Shoulder and Elbow Surgeons score and the health status questionnaire 12. Recurrent instability persisted in four patients after the revision arthroscopic procedure. At the final follow-up, the mean American Shoulder and Elbow Surgeons score was 81.1 (17.5 to 99.5) and the mean Western Ontario shoulder instability index score was 68.2 (20 to 98.2). Quality-of-life scoring showed good to excellent results in most patients. Arthroscopic revision capsulolabral reconstruction can provide a satisfactory outcome in selected patients for recurrent instability of the shoulder provided that no large Hill-Sachs lesion is present.
The epidemiology of fractures in adolescents with reference to social deprivation
Authors: Menon M. R. G. , Walker J. L. , Court-Brown C. M. 
Published: November 2008

A relationship between social deprivation and the incidence of fracture in adolescents has not previously been shown. We have used a complete fracture database to identify adolescents who sustained fractures in 2000. The 2001 Scottish census was used to obtain age-specific population and deprivation data according to the Carstairs score. Regression analysis determined the relationship between the incidence of fractures and social deprivation. We analysed 1574 adolescents with fractures (1083 male, 491 female). The incidence of fractures in this group was 21.8 per thousand (31.0 male, 13.1 female). Social deprivation predicted the incidence in adolescent males and females. The incidence of fractures of the proximal upper limb and distal radius in females was overwhelmingly influenced by socioeconomic factors. Males of 15 to 20 years of age were more likely to sustain fractures of the hand and carpus if they lived in economically depressed neighbourhoods.
Reconstruction of the anterior cruciate ligament in patients over 50 years
Authors: Dahm D. L. , Wulf C. A. , Dajani K. A. , Dobbs R. E. , Levy B. A. , Stuart M. A. 
Published: November 2008

The records of patients aged 50 years or over who underwent primary reconstruction of the anterior cruciate ligament between 1990 and 2002 were reviewed. There were 35 knees in 34 patients that met the inclusion criteria. The mean age of the patients was 57 years (50 to 66) and the mean clinical follow-up was for 72 months (25 to 173). A total of 23 knees were reconstructed with patellar tendon allograft, and 12 with patellar tendon autograft. The mean pre-operative knee extension was 1° (−5° to 10°) and flexion was 129° (125° to 150°) and at follow-up these values were 0° (−5° to 5°) and 135° (120° to 150°), respectively. Pre-operatively there were 31 knees (89%) with a Lachman grade 2+ or 3+. Post-operatively, 33 knees (94%) were Lachman grade 0 or 1+. The mean pre- and post-operative International Knee Documentation Committee scores were 39 (23 to 72) and 90 (33 to 100) respectively. The mean pre- and post-operative Lysholm scores were 50 (18 to 68) and 92 (28 to 100) respectively and the mean University of California Los Angeles activity scores were 8.5 before injury (4 to 10), 4.3 (3 to 6) after injury and 8.3 (4 to 10) post-operatively. There were three graft failures (8.6%) requiring revision. We conclude that reconstruction of the anterior cruciate ligament in carefully-selected patients aged 50 years or over can achieve similar results to those in younger patients, with no increased risk of complications.
Development of new buffer layers for Cu(In,Ga)Se2 solar cells
Authors: Ahn Byung Tae , Larina Liudmila , Kim Ki Hwan , Ahn Soong Ji 
Published: November 2008

Abstract
Recent progress in the field of Cu(In,Ga)Se2 (CIGS) thin film solar cell technology is briefly reviewed. New wide-bandgap In
x
(OOH,S)
y
and ZnS
x
(OH)
y
O
z
buffers for CIGS solar cells have been developed. Advances have been made in the film deposition by the growth process optimization that allows the control of film properties at the micro- and nanolevels. To improve the CIGS cell junction characteristics, we have provided the integration of the developed Cd-free films with a very thin CdS film. Transmittances of the developed buffers were greatly increased compared to the standard CdS. In
x
(OOH,S)
y
buffer has been applied to low-bandgap CIGS devices which have shown poor photovoltaic properties. The experimental results obtained suggest that low efficiency can be explained by unfavorable conduction band alignment at the In
x
(OOH,S)
y
/CIGS heterojunction. The application of a wide-gap Cu(In,Ga)(Se,S)2 absorber for device fabrication yields the conversion efficiency of 12.55 %. As a result, the In
x
(OOH,S)
y
buffer is promising for wide-bandgap Cu(In,Ga)(Se,S)2 solar cells, however, its exploration for low-bandgap CIGS devices will not allow a high conversion efficiency. The role played by interdiffusion at the double-buffer/CIGS heterojunction and its impact on the electronic structure and device performance has also been discussed.
Approaches to low-bandgap polymer solar cells: Using polymer charge-transfer complexes and fullerene metallocomplexes
Authors: Zapunidy Sergey A. , Martyanov Dmitry S. , Nechvolodova Elena M. , Tsikalova Marina V. , Novikov Yuri N. , Paraschuk Dmitry Yu. 
Published: November 2008

Abstract
Polymer solar cells have shown high potential to convert solar energy into electricity in a cost-effective way. One of the basic reasons limiting the polymer solar cell efficiency is insufficient absorption of the solar radiation by the active layer that limits the photocurrent. To increase the photocurrent, one needs low-bandgap materials with strong absorption below 2 eV. In this work, we study two types of low-bandgap materials: ground-state charge-transfer complexes (CTCs) of a conjugated polymer, MEH-PPV (poly[2-methoxy-5-(2'-ethyl-hexyloxy)-1,4-phenylenevinylene]), and an exohedral metallocomplex of fullerene, (η2-C60)IrH(CO)[(+)DIOP] (IrC60). We demonstrate that the CTC formed between MEH-PPV and conjugated molecules with high electron affinity, namely, 2,4,7-trinitrofluorenone (TNF) and 1,5-dinitroantraquinone (DNAQ), can have strong optical absorption extending down to the near infrared. We have observed that the photoexcited CTC can generate free charges. We also report on optical studies of IrC60 as a possible acceptor for polymer/fullerene solar cells. IrC60 strongly absorbs in the visible spectral range, in particular in the red part, and therefore has a potential for increasing the photocurrent as compared with polymer/methanofullerene solar cells. Our studies of MEH-PPV/IrC60 blended films show that long-lived charges are efficiently generated at MEH-PPV upon photoexcitation of the blend.