bluuebunny/crossref_metadata_2025_split huggingface dataset:
PELATIHAN BISNIS CANVAS MODEL DAN SOSIALISASI APLIKASI CIS SMESCO UNTUK UMKM SEBAGAI UPAYA KESIAPAN GO PUBLIC DI BANGKA BELITUNG
Authors: Sumiyati Sumiyati , Faisal Akbar Muhammad 
Published: March 2024

The canvas business model training program and the socialization of the SMESCO CIS application aim to provide education for MSME actors to manage their business. The business canvas model is a business model that helps business actors in designing their business. CIS SMESCO itself is an integrated business application for Cooperatives and MSMEs in Indonesia. This program is very important as a form of support for the Government in realizing Cooperatives and MSMEs to go digital as well as go public.
The method of implementing this training uses the lecture method. The participants were 50 people who were MSME actors who had just started their business. The activity is carried out online in approximately 90 minutes. Evaluation of activities was carried out by distributing feedback questionnaires using google forms a few minutes before the event took place. The results of the evaluation showed that 80% of the participants considered this activity very informative and understood the material presented by the resource persons.
This activity is beneficial for MSME actors to transform digitally and in an effort to go public. This activity needs to be carried out offline by helping participants to start digitizing their business.
Tradisi Ngalangkang Pambak pada Umat Hindu Kaharingan di Kecamatan Katingan Hilir Kabupaten Katingan (Kajian Agama Budaya dan Psikologi)
Authors: Eka Nali , Megawati Megawati , Wiranata Anak Agung Gede , Vienlentia Raisa 
Published: March 2024

Abstrak
Upacara kematian yang dilaksanakan oleh umat Hindu Kaharingan di Kecamatan Katingan Hilir terdiri dari tahapan upacara penguburan, Tiwah dan Ngalangkang Pambak. Upacara ini merupakan upacara wajib lanjutan setelah dilaksanakan upacara Tiwah, namun kenyataan di lapangan Ngalangkang Pambak ini tidak lagi hanya dilaksanakan setelah upacara. Upacara ini bahkan dilaksanakan sebagai ritual tahunan atau pun untuk memperingati peristiwa tertentu. Penelitian ini menggunakan metode kualitatif dengan pendekatan studi kasus. Jenis data penelitian ini yakni data primer yang bersumber dari para informan dan data sekunder dari data pendukung. Metode pengumpulan data meliputi pengamatan, wawancara, perekaman dan studi dokumentasi. Prosedur analisis dan interpretasi data dalam penelitian dengan pendekatan “ground up”. Hasil dari penelitian ini menunjukan bahwa Ngalangkang Pambak merupakan bagian dari rangkaian pelaksanaan upacara Tiwah. Namun dalam perkembangannya berubah menjadi tradisi tahunan. Ngalangkang Pambak menjadi acara ucapan syukur syukur atas kesejahteraan, rejeki, kesehatan yang sudah diberikan oleh para leluhur dengan cara membersihkan Pambak atau kubur mendiang keluarga (leluhur) yang sudah meninggal. Ngalangkang Pambak juga sebagai sarana membayar hajat karena segala permintaan baik berupa pekerjaaan atau pencapaian yang diminta secara khusus kepada leluhur telah tercapai semua. Pelaksanaan Ngalangkang Pambakjuga berkaitan dengan emosional keluarga dimana tradisi Ngalangkang Pambak dilakukan secara turun temurun dalam suatu keluarga. Dalam ritual Ngalangkang Pambak memunculkan adanya rasa senang dan membuat hidup lebih lega dan menyenangkan membawa sukacita bagi keluarga yang telah melaksanakan karena ritual ini juga berarti kelurga dinilai mampu untuk melaksanakan bentuk bakti kepada para leluhur.
A comparison of conventional and advanced 3D imaging techniques for percutaneous left atrial appendage closure
Authors: Heidari Houtan , Kanschik Dominika , Maier Oliver , Wolff Georg , Brockmeyer Maximilian , Masyuk Maryna , Bruno Raphael Romano , Polzin Amin , Erkens Ralf , Antoch Gerald , Reinartz Sebastian Daniel , Werner Nikos , Kelm Malte , Zeus Tobias , Afzal Shazia , Jung Christian 
Published: March 2024

BackgroundUnderstanding complex cardiac anatomy is essential for percutaneous left atrial appendage (LAA) closure. Conventional multi-slice computed tomography (MSCT) and transesophageal echocardiography (TEE) are now supported by advanced 3D printing and virtual reality (VR) techniques for three-dimensional visualization of volumetric data sets. This study aimed to investigate their added value for LAA closure procedures.MethodsTen patients scheduled for interventional LAA closure were evaluated with MSCT and TEE. Patient-specific 3D printings and VR models were fabricated based on MSCT data. Ten cardiologists then comparatively assessed LAA anatomy and its procedure relevant surrounding structures with all four imaging modalities and rated their procedural utility on a 5-point Likert scale questionnaire (from 1 = strongly agree to 5 = strongly disagree).ResultsDevice sizing was rated highest in MSCT (MSCT: 1.9 ± 0.8; TEE: 2.6 ± 0.9; 3D printing: 2.5 ± 1.0; VR: 2.5 ± 1.1; p &lt; 0.01); TEE, VR, and 3D printing were superior in the visualization of the Fossa ovalis compared to MSCT (MSCT: 3.3 ± 1.4; TEE: 2.2 ± 1.3; 3D printing: 2.2 ± 1.4; VR: 1.9 ± 1.3; all p &lt; 0.01). The major strength of VR and 3D printing techniques was a superior depth perception (VR: 1.6 ± 0.5; 3D printing: 1.8 ± 0.4; TEE: 2.9 ± 0.7; MSCT: 2.6 ± 0.8; p &lt; 0.01). The visualization of extracardiac structures was rated less accurate in TEE than MSCT (TEE: 2.6 ± 0.9; MSCT: 1.9 ± 0.8, p &lt; 0.01). However, 3D printing and VR insufficiently visualized extracardiac structures in the present study.ConclusionA true 3D visualization in VR or 3D printing provides an additional value in the evaluation of the LAA for the planning of percutaneous closure. In particular, the superior perception of depth was seen as a strength of a 3D visualization. This may contribute to a better overall understanding of the anatomy. Clinical studies are needed to evaluate whether a more comprehensive understanding through advanced multimodal imaging of patient-specific anatomy using VR may translate into improved procedural outcomes.
Association of metabolic syndrome and sarcopenia with all-cause and cardiovascular mortality: a prospective cohort study based on the NHANES
Authors: Huang Weihong , Deng Siyi , Liu Siyang , Ma Qintao , Cao Liting , Liu Lan , Wan Heng , Shen Jie 
Published: March 2024

BackgroundMetabolic syndrome (MetS) and sarcopenia (SP) have emerged as significant public health concerns in contemporary societies, characterized by shared pathophysiological mechanisms and interrelatedness, leading to profound health implications. In this prospective cohort study conducted within a US population, we aimed to examine the influence of MetS and SP on all-cause and cardiovascular mortality.MethodsThis study analyzed data from the National Health and Nutrition Examination Survey (NHANES) III for the years 1999-2006 and 2011-2018, and death outcomes were ascertained by linkage to National Death Index (NDI) records through December 31, 2019. Cox proportional hazard models were used to estimate hazard ratios (HRs) and 95% confidence intervals (95% CIs) for all-cause and cardiovascular mortality. In addition, subgroup and sensitivity analyses were conducted to test the robustness of the results.ResultsOver a median follow-up period of 13.3 years (95% CI: 12.8-13.8), 1714 deaths were observed. The groups characterized by MetS−/SP+, MetS+/SP−, and MetS+/SP+ exhibited higher all-cause mortality rates in comparison to the MetS-/SP- group, with the MetS+/SP+ group (HR 1.76, 95% CI: 1.37-2.25) displaying the highest all-cause mortality. Increased cardiovascular mortality was observed in the MetS+/SP− (HR 1.84, 95% CI: 1.24-2.72), and MetS+/SP+ groups (HR 2.39, 95% CI: 1.32-4.35) compared to the MetS−/SP− group, whereas it was not statistically significant in the MetS-/SP+ group. However, among males and individuals aged &lt; 60, the presence of both MetS and SP (MetS+/SP+ group) was found to be significantly associated with a higher risk of all-cause and cardiovascular mortality.ConclusionThe coexistence of MetS and SP increased the risk of all-cause and cardiovascular mortality, particularly in males and in nonelderly populations. Individuals with either MetS or SP may require more careful management to prevent the development of other diseases and thereby reduce mortality.
Influence of the number of washings for embryos on non-invasive preimplantation chromosome screening results
Authors: Kang Xiaomei , Wen Meiting , Zheng Jie , Peng Fangxin , Zeng Ni , Chen Zhu , Wu Yanting , Sun Hong 
Published: March 2024

ObjectiveTo explore the effect of varying numbers of embryo washings prior to blastocyst formation in non-invasive preimplantation chromosome screening (NICS) on the accuracy of NICS results.MethodsIn this study, 68 blastocysts from preimplantation genetic testing (PGT)-assisted pregnancy were collected at our institution. On the fourth day of embryo culture, the embryos were transferred to a new medium for blastocyst culture and were washed either three times (NICS1 group) or ten times (NICS2 group). A trophectoderm (TE) biopsy was performed on the blastocysts, and the corresponding embryo culture media were collected for whole genome amplification (WGA) and high-throughput sequencing.ResultsThe success rate of WGA was 100% (TE biopsy), 76.7% (NICS1 group), and 89.5% (NICS2 group). The success rate of WGA in embryo medium on days 5 and 6 of culture was 75.0% (33/44) and 100% (24/24), respectively. Using TE as the gold standard, the karyotype concordance rate between the results of the NICS1 and NICS2 groups’ embryo culture medium samples and TE results was 43.5% (10/23) and 73.5% (25/34), respectively. The sensitivity and specificity of detecting chromosomal abnormalities were higher in the NICS2 group than in the NICS1 group when TE was used (83.3% vs 60.0%; 62.5% vs 30.8%, respectively). The false-positive rate and false-negative rate (i.e., misdiagnosis rate and missed diagnosis rate, respectively) were lower in the NICS2 group than in the NICS1 group (37.5% vs 69.2%; 16.7% vs 40.0%, respectively).ConclusionThe NICS yielded favorable results after ten washings of the embryos. These findings provide a novel method for lowering the amount of cell-free DNA contamination from non-embryonic sources in the medium used for embryo development, optimizing the sampling procedure and improving the accuracy of the NICS test.
Association of adrenal steroids with metabolomic profiles in patients with primary and endocrine hypertension
Authors: Knuchel Robin , Erlic Zoran , Gruber Sven , Amar Laurence , Larsen Casper K. , Gimenez-Roqueplo Anne-Paule , Mulatero Paolo , Tetti Martina , Pecori Alessio , Pamporaki Christina , Langton Katharina , Peitzsch Mirko , Ceccato Filippo , Prejbisz Aleksander , Januszewicz Andrzej , Adolf Christian , Remde Hanna , Lenzini Livia , Dennedy Michael , Deinum Jaap , Jefferson Emily , Blanchard Anne , Zennaro Maria-Christina , Eisenhofer Graeme , Beuschlein Felix 
Published: March 2024

IntroductionEndocrine hypertension (EHT) due to pheochromocytoma/paraganglioma (PPGL), Cushing’s syndrome (CS), or primary aldosteronism (PA) is linked to a variety of metabolic alterations and comorbidities. Accordingly, patients with EHT and primary hypertension (PHT) are characterized by distinct metabolic profiles. However, it remains unclear whether the metabolomic differences relate solely to the disease-defining hormonal parameters. Therefore, our objective was to study the association of disease defining hormonal excess and concomitant adrenal steroids with metabolomic alterations in patients with EHT.MethodsRetrospective European multicenter study of 263 patients (mean age 49 years, 50% females; 58 PHT, 69 PPGL, 37 CS, 99 PA) in whom targeted metabolomic and adrenal steroid profiling was available. The association of 13 adrenal steroids with differences in 79 metabolites between PPGL, CS, PA and PHT was examined after correction for age, sex, BMI, and presence of diabetes mellitus.ResultsAfter adjustment for BMI and diabetes mellitus significant association between adrenal steroids and metabolites – 18 in PPGL, 15 in CS, and 23 in PA – were revealed. In PPGL, the majority of metabolite associations were linked to catecholamine excess, whereas in PA, only one metabolite was associated with aldosterone. In contrast, cortisone (16 metabolites), cortisol (6 metabolites), and DHEA (8 metabolites) had the highest number of associated metabolites in PA. In CS, 18-hydroxycortisol significantly influenced 5 metabolites, cortisol affected 4, and cortisone, 11-deoxycortisol, and DHEA each were linked to 3 metabolites.DiscussionsOur study indicates cortisol, cortisone, and catecholamine excess are significantly associated with metabolomic variances in EHT versus PHT patients. Notably, catecholamine excess is key to PPGL’s metabolomic changes, whereas in PA, other non-defining adrenal steroids mainly account for metabolomic differences. In CS, cortisol, alongside other non-defining adrenal hormones, contributes to these differences, suggesting that metabolic disorders and cardiovascular morbidity in these conditions could also be affected by various adrenal steroids.
Loss of native brown trout diversity in streams of the continental Croatia
Authors: Kanjuh Tamara , Marić Ana , Škraba Jurlina Dubravka , Simonović Predrag , Špelić Ivan , Piria Marina , Maguire Ivana 
Published: March 2024

Introduction: The genetic diversity of brown trout in the Western Balkans has been disrupted by the introduction of non-native Atlantic phylogenetic lineages and non-native haplotypes of the Danubian phylogenetic lineage. The Western Balkans is characterized by the greatest phenotypic and genotypic diversity of trout populations, and a large part of the internal territory belongs to the Black Sea basin, where the Danubian Da1 haplotype is native. Artificial propagation of non-native lineages in the Western Balkans has a long history, and these populations are often the only available material for stocking rivers attractive for fishing.Material and Methods: Fifteen populations in the Danube basin of the continental Croatia were analysed. The analysis of eight microsatellite loci was performed to determine the structure of brown trout populations, as well as the degree of introgression of non-native genetic material into the native.Results and Disscusion: The results of this study showed significant genetic similarity among brown trout populations, confirming a long history of introduction with non-native genetic material. The main reason was uncontrolled stocking with inadequate material, which is available in fish farms and consists mainly of brown trout of the Atlantic phylogenetic lineage. The results of this study also indicated stocking with brown trout of the non-native haplotypes of the Danubian phylogenetic lineage. The potential breeding origin of brown trout carrying the Danubian Da2 mtDNA haplotype and ways of its introduction into rivers have yet to be investigated. For the survival of the unique gene pool of brown trout in Croatian rivers, it is of fundamental importance to know the structure of wild and farmed populations with the aim of proposing and implementing conservation measures.
Deconvolution of synovial myeloid cell subsets across pathotypes and role of COL3A1+ macrophages in rheumatoid arthritis remission
Authors: Hu Xuantao , Zhang Ziji , Long Lingli , Gu Minghu , Chen Weishen , Pan Baiqi , Wu Xiaoyu , Wang Chao , Li Chengxin , Zheng Linli , Sheng Puyi 
Published: March 2024

BackgroundMonocyte/macrophage (Mo/Mp) is a critical cell population involved in immune modulation of rheumatoid synovitis (RA) across different pathotypes. This study aims to investigate the contribution of Mo/Mp clusters to RA activity, and the biological function of particular subtypes in RA remission.MethodsWe integrated single-cell RNA sequencing datasets from 4 published and 1 in-house studies using Liger selected by comparison. We estimated the abundance of Mo/Mp subtypes in bulk RNA-seq data from the 81 patients of the Pathobiology of Early Arthritis Cohort (PEAC) using deconvolution analysis. Correlations between Mo/Mp subtypes and RA clinical metrics were assessed. A particular cell type was identified using multicolor immunofluorescence and flow cytometry in vivo and successfully induced from a cell line in vitro. Potential immune modulation function of it was performed using immunohistochemical staining, adhesion assay, and RT-qPCR.ResultsWe identified 8 Mo/Mp clusters. As a particular subtype among them, COL3A1+ Mp (CD68+, COL3A1+, ACTA2-) enriched in myeloid pathotype and negatively correlated with RA severity metrics in all pathotypes. Flow cytometry and multicolor immunofluorescence evidenced the enrichment and M2-like phenotype of COL3A1+ Mp in the myeloid pathotype. Further assays suggested that COL3A1+ Mp potentially attenuates RA severity via expressing anti-inflammatory cytokines, enhancing Mp adhesion, and forming a physical barrier at the synovial lining.ConclusionThis study reported unexplored associations between different pathologies and myeloid cell subtypes. We also identified a fibroblast-and-M2-like cluster named COL3A1+ Mp, which potentially contributes to synovial immune homeostasis. Targeting the development of COL3A1+ Mp may hold promise for inducing RA remission.
Immature neutrophils in cord blood exert increased expression of genes associated with antimicrobial function
Authors: Miková Eliška , Černý Viktor , Novotná Olga , Petrásková Petra , Boráková Kristýna , Hel Zdenek , Hrdý Jiří 
Published: March 2024

IntroductionThe immune systems of both the mother and the newborn face significant challenges during birth. Proper immune regulation after birth is essential for the survival of neonates. Numerous studies have demonstrated that the neonatal immune system is relatively immature, particularly in its adaptive arm, placing the primary responsibility for immune surveillance on innate immunity.MethodsGiven the significant role of neutrophils in protecting the neonate after birth, we conducted a study investigating the properties of neutrophils in newborn cord blood using various methodological approaches.ResultsOur findings demonstrate the presence of immature low-density neutrophils in the cord blood, which are likely responsible for the observed elevated expression of genes coding for proteins essential to antimicrobial response, including myeloperoxidase, neutrophils elastase, and defensins.DiscussionWe propose that these cells function normally and support the protection of newborns early after birth. Furthermore, our results suggest that the mode of delivery might significantly influence the programming of neutrophil function. The presented findings emphasize the importance of distinct neutrophil subpopulations in neonatal immunity and their potential impact on early postnatal health.
Human surfactant protein A inhibits SARS-CoV-2 infectivity and alleviates lung injury in a mouse infection model
Authors: Jacob Ikechukwu B. , Gemmiti Amanda , Xiong Weichuan , Reynolds Erin , Nicholas Brian , Thangamani Saravanan , Jia Hongpeng , Wang Guirong 
Published: March 2024

IntroductionSARS coronavirus 2 (SARS-CoV-2) infects human angiotensin-converting enzyme 2 (hACE2)-expressing lung epithelial cells through its spike (S) protein. The S protein is highly glycosylated and could be a target for lectins. Surfactant protein A (SP-A) is a collagen-containing C-type lectin, expressed by mucosal epithelial cells and mediates its antiviral activities by binding to viral glycoproteins.ObjectiveThis study examined the mechanistic role of human SP-A in SARS-CoV-2 infectivity and lung injury in vitro and in vivo.ResultsHuman SP-A can bind both SARS-CoV-2 S protein and hACE2 in a dose-dependent manner (p&lt;0.01). Pre-incubation of SARS-CoV-2 (Delta) with human SP-A inhibited virus binding and entry and reduced viral load in human lung epithelial cells, evidenced by the dose-dependent decrease in viral RNA, nucleocapsid protein (NP), and titer (p&lt;0.01). We observed significant weight loss, increased viral burden, and mortality rate, and more severe lung injury in SARS-CoV-2 infected hACE2/SP-A KO mice (SP-A deficient mice with hACE2 transgene) compared to infected hACE2/mSP-A (K18) and hACE2/hSP-A1 (6A2) mice (with both hACE2 and human SP-A1 transgenes) 6 Days Post-infection (DPI). Furthermore, increased SP-A level was observed in the saliva of COVID-19 patients compared to healthy controls (p&lt;0.05), but severe COVID-19 patients had relatively lower SP-A levels than moderate COVID-19 patients (p&lt;0.05).DiscussionCollectively, human SP-A attenuates SARS-CoV-2-induced acute lung injury (ALI) by directly binding to the S protein and hACE2, and inhibiting its infectivity; and SP-A level in the saliva of COVID-19 patients might serve as a biomarker for COVID-19 severity.
Retinal detachment with multiple macrocysts in Stickler syndrome: case report and review of the literature
Authors: Liu Guina , Hu Ming , Cai Chengcheng , Jiang Xiaoshuang , Lu Fang 
Published: March 2024

BackgroundStickler syndrome is a hereditary connective tissue disorder associated with ocular, orofacial, musculoskeletal, and auditory impairments. Its main clinical characteristics include retinal detachment, hearing loss, and midface underdevelopment. In clinical practice, macrocyst is rarely reported in retinal detachment cases with Stickler syndrome.Case presentationWe report the case of a 7-year-old child who developed a rhegmatogenous retinal detachment (RRD) in the right eye, accompanied by multiple peripheral macrocysts. The detachment was successfully surgically repaired with vitrectomy, retinal laser photocoagulation, cryotherapy and silicone oil tamponade. During the operation, a mini-retinectomy in the outer layer of each macrocyst was made for vesicular drainage and retinal reattachment. Genetic testing identified a pathogenic point mutation variant (c.1693C&gt;T; p.Arg565Cys) in exon 26 of the COL2A1 gene. Six-months after the operation, the retina remained attached with improvement of best corrected visual acuity to 20/200.ConclusionPatients with Stickler syndrome may develop RRD of different severity. Macrocyst is rarely reported in previous literature of Stickler syndrome. In this case report, we share our experience in treating with multiple macrocysts in RRD and emphasize the importance of periodic follow-up for patients with Stickler syndrome.
Bacillus coagulans regulates gut microbiota and ameliorates the alcoholic-associated liver disease in mice
Authors: Liu Zhenzhen , Liu Tong , Zhang Zhenting , Fan Yurong 
Published: March 2024

IntroductionAlcoholic-associated liver diseases (ALD) are now widespread issues worldwide. Alcoholic-induced chronic dysbiosis of the gut microbiota is one of the factors in the pathophysiology of ALD.MethodsIn this work, we employed a chronic-binge ethanol feeding mice model, as described in a previous report.ResultsOur findings demonstrate that hepatic inflammatory injury damage and accumulation of fat can be effectively reduced in mice with ALD by altering the gut microbiota utilizing Bacillus coagulans. Treatment with B. coagulans significantly modulates the levels of TNF-α, IL-1β, and IL-22 cytokines while maintaining tight junction proteins and mucin protein expressions to support intestinal barrier function restoration. Treatment with B. coagulans also alters the composition of the gut microbiota and increases the production of short-chain fatty acids (SCFAs).DiscussionThis is mostly due to B. coagulans promotes the growth of bacteria that produce SCFAs, such as Ruminococcus species and Akkermansia, while inhibiting the growth of pathogenic bacteria like Escherichia Shigella. Moreover, treatment with B. coagulans causes levels of 2-Ketobutyric acid, ketoleucine, and indoleacetic acid increase while homovanillic acid and 3’-O-Methylguanosine metabolites decrease significantly. This study facilitates the development of therapeutic and preventive strategies for ALD using lactic acid bacteria.