bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta
Authors: Zhang Huiming , Xie Xitao , Kim Mi‐Seong , Kornyeyev Dmytro A. , Holaday Scott , Paré Paul W. 
Published: June 2008

SummaryPhotosynthesis is regulated by environmental factors as well as endogenous sugar signals. Whereas light‐driven sugar biosynthesis is essential for terrestrial organisms, as well as belowground microflora, whether and how soil symbionts regulate photosynthesis has yet to be reported. Here, we show that the plant growth‐promoting soil bacterium Bacillus subtilis GB03 augments photosynthetic capacity by increasing photosynthetic efficiency and chlorophyll content in Arabidopsis. Mechanistic studies reveal an elevation of sugar accumulation as well as the suppression of classic glucose signaling responses, including hypocotyl elongation and seed germination, with exposure to GB03. Compared with wild‐type plants, two Arabidopsis mutants defective in hexokinase‐dependent sugar signaling exhibit increased photosynthetic capacity, which is not further enhanced with GB03 exposure. Overlap in sugar/ABA sensing is observed in GB03‐exposed plants, with a reduction of ABA‐biosynthetic transcripts as well as downstream metabolite levels in leaves. Moreover, exogenous ABA abrogates GB03‐triggered increases in photosynthetic efficiency and chlorophyll content. These results demonstrate that certain rhizobacteria elevate photosynthesis through the modulation of endogenous sugar/ABA signaling, and establish a regulatory role for soil symbionts in plant acquisition of energy.
Stimulus‐induced downregulation of root water transport involves reactive oxygen species‐activated cell signalling and plasma membrane intrinsic protein internalization
Authors: Boursiac Yann , Boudet Julie , Postaire Olivier , Luu Doan‐Trung , Tournaire‐Roux Colette , Maurel Christophe 
Published: June 2008

SummaryThe water uptake capacity of plant roots (i.e. their hydraulic conductivity, Lpr) is determined in large part by aquaporins of the plasma membrane intrinsic protein (PIP) subfamily. In the present work, we investigated two stimuli, salicylic acid (SA) and salt, because of their ability to induce an accumulation of reactive oxygen species (ROS) and an inhibition of Lpr concomitantly in the roots of Arabidopsis plants. The inhibition of Lpr by SA was partially counteracted by preventing the accumulation of hydrogen peroxide (H2O2) with exogenous catalase. In addition, exogenous H2O2 was able to reduce Lpr by up to 90% in <15 min. Based on the lack of effects of H2O2 on the activity of individual aquaporins in Xenopus oocytes, and on a pharmacological dissection of the action of H2O2 on Lpr, we propose that ROS do not gate Arabidopsis root aquaporins through a direct oxidative mechanism, but rather act through cell signalling mechanisms. Expression in transgenic roots of PIP‐GFP fusions and immunogold labelling indicated that external H2O2 enhanced, in <15 min, the accumulation of PIPs in intracellular structures tentatively identified as vesicles and small vacuoles. Exposure of roots to SA or salt also induced an intracellular accumulation of the PIP‐GFP fusion proteins, and these effects were fully counteracted by co‐treatment with exogenous catalase. In conclusion, the present work identifies SA as a novel regulator of aquaporins, and delineates an ROS‐dependent signalling pathway in the roots of Arabidopsis. Several abiotic and biotic stress‐related stimuli potentially share this path, which involves an H2O2‐induced internalization of PIPs, to downregulate root water transport.
Multiple eruptive dermatofibromas and immunosuppression: report of two cases and review of the literature
Authors: Zaccaria E. , Rebora A. , Rongioletti F. 
Published: June 2008

AbstractDermatofibromas are common benign fibrohistiocytic tumors that are most often solitary. The occurrence of multiple eruptive dermatofibromas (MEDF), on the contrary, is a rare event. MEDF have been reported in the setting of autoimmune diseases, treated with immunosuppressive drugs, in the course of HIV infection and in neoplastic diseases. An association with immunosuppression has led to the speculation that they are the result of an abortive immunoreactive process. Here, we describe a patient with Sézary syndrome and a patient with multiple IgA myeloma who developed MEDF. These associations have not been reported previously.
Rhinosporidiosis: gigantic cells with engulfed sporangia of Rhinosporidium seeberi in the case of dermosporidiosis
Authors: Ghorpade Ashok 
Published: June 2008

AbstractRhinosporidiosis in an Indian male with a tumorous skin lesion is reported. Histopathology from the skin lesion revealed gigantic foreign body giant cells bloated with multiple, engulfed sporangia of rhinosporidiosis, which has not been reported earlier. The term “dermosporidiosis”, should be used to denote such predominant skin lesions of rhinosporidiosis.
Lichenoid inflammation in vitiligo – a clinical and histopathologic review of 210 cases*
Authors: Attili Venkat Ratnam , Attili Sasi Kiran 
Published: June 2008

AbstractBackground  ‘“Inflammatory vitiligo” cases with clear lichenoid infiltrates have been reported. However, the inflammatory nature of common vitiligo has not gained wide acceptance because of its benign appearance and scanty cellular infiltrates. We have observed in our patients a few lesions with mild erythema, scaling and marginal hyperpigmentation which were suspected to be inflammatory. This study was conducted to assess the histological features and prevalence of such marginally active lesions, in comparison with common vitiligo.Methods  Two hundred and ten consecutive new cases of vitiligo seeking treatment for the first time were included in this study. Clinical lesions were carefully examined and biopsies were taken in all cases. Biopsies were also taken from pigmented skin 3 cm away from the vitiligo lesion in 20 cases and normal pigmented skin over the contralateral side in 20.Results  Marginally active lesions with erythema, scaling and hyperpigmentation were identified in 27 patients (13%). Lymphocytic infiltration of dermo‐epidermal interface was observed in 89% of these cases which was clearly lichenoid in 59%. Similar lichenoid infiltrates were also seen in 50% of pigmented skin samples 3 cm away from the lesion and 23% of common macular vitiligo lesions.Conclusions  Vitiligo is an inflammatory disease that with development involves a lichenoid tissue reaction.
Eponyms of Sir Jonathan Hutchinson
Authors: Van Ruth Serge , Toonstra Johan 
Published: June 2008

AbstractSir Jonathan Hutchinson was an extraordinary man. He was trained as a surgeon and a pathologist, but was also keenly interested in dermatology, the study of syphilis, ophthalmology, and neurology. His observations with detailed descriptions of skin diseases were remarkable. His medical bibliography staggeringly consists of over 1000 published reports. This description of the eponyms attributed to Hutchinson – illustrated with clinical images, an old plate, and a portrait – demonstrates his important contribution to dermatology.
Oral infection by Diptera larvae in children: a case report
Authors: De Souza Barbosa Taís , Salvitti Sá Rocha Renata Andréa , Guirado Cecília Gatti , Rocha Fábio Jordão ,   Graduate student, Duarte Gavião Maria Beatriz 
Published: June 2008

AbstractBackground  Oral myiasis is usually caused by flies of the order Diptera. One of the causes of human myiasis is Cochliomyia hominivorax, which is a true obligate parasite of mammals.Methods  A case of oral myiasis in a 5‐year‐old girl is reported. She presented with a swelling on the hard palate accompanied by intense pain and a fetid odor. A literature review of oral myiasis reports in children was performed.Results  The poor oral hygiene, lip incompetence, open bite, and residence in a rural area were considered to be predisposing factors for larval infestation in this patient. Treatment consisted of manual larval removal and systemic therapy with antimicrobials.Conclusions  The prevention of human myiasis should involve the control of fly populations and general cleanliness, such as reducing decomposition odors and cleaning and covering wounds. In addition, the public should be informed that individuals living in locations without basic sanitation are more predisposed to infestation.
Comparison of time use, role participation and life satisfaction of older people after stroke with a sample without stroke
Authors: McKenna Kryss , Liddle Jacki , Brown Alysha , Lee Katherine , Gustafsson Louise 
Published: June 2008

Background/aims:  This study investigated the time use, role participation and life satisfaction of older Australians (aged 65 years and older) who were 1–3 years post‐stroke and living in the community. The results of this study were compared with a published study on the time use, role participation and life satisfaction of older Australians who had not experienced stroke. Methods:  Twenty‐three participants with stroke (mean age 74.2 years, 69.6% men) were interviewed using measures of time use, role participation and life satisfaction. Results:  Participants with stroke spent most of their time in sleep (7.2 h/day), solitary leisure (7.0 h/day), social leisure (3.0 h/day), and basic activities of daily living (2.9 h/day). Compared to the sample without stroke, participants with stroke spent significantly less time in sleep, instrumental activities of daily living, and volunteer work, and significantly more time at home, with others, and engaged in solitary leisure. Similar to the sample without stroke, the most common roles for participants with stroke were family member, friend, and home maintainer. Participants with stroke engaged in fewer roles than participants without stroke. Unlike the sample without stroke, role loss was not correlated with life satisfaction for participants with stroke; however, having more roles was correlated with greater life satisfaction. Conclusion:  Experiencing a stroke can affect the configuration of older people's time use and reduce their role participation. Facilitation of older people's role participation after stroke may enhance their life satisfaction.
The Tree Theme Method as an intervention in psychosocial occupational therapy: Client acceptability and outcomes
Authors: Gunnarsson A. Birgitta , Eklund Mona 
Published: June 2008

Background/aim: The Tree Theme Method (TTM) is an intervention in which the client paints trees representing certain periods in his/her life. The intervention comprises five sessions, using trees as a starting point to tell one's life story. This study, which is part of an implementation project, aimed to examine the therapeutic alliance and client satisfaction, in relation to perceptions of everyday occupations and health‐related factors, with clients going through a TTM intervention.Methods: Nine occupational therapists recruited 35 clients, at general outpatient mental health care units, for the TTM intervention. Self‐rating instruments, targeting therapeutic alliance (HAq‐II), different aspects of daily occupations (Canadian Occupational Performance Measure, Satisfaction with Daily Occupations), health‐related factors (Sense of Coherence measure, Mastery Scale, Symptom Checklist‐90‐R) and client satisfaction (Client Satisfaction Questionnaire), were administrated before and after the intervention.Results: A good initial therapeutic alliance, experienced by both therapists and clients, was correlated to increased changes regarding occupational performance and self‐mastery. According to the therapists’ ratings, a good initial therapeutic alliance was correlated to increased sense of coherence and a decreased level of psychiatric symptoms. The results showed positive significant changes in occupational performance and health‐related factors. High ratings of the therapeutic alliance by the therapists were also related to high client satisfaction.Conclusions: The TTM seemed to function well in psychosocial occupational therapy, but there is a need for further implementation studies to deepen our understanding of the treatment process, comprising both technique and formation of the therapeutic alliance.
Doppler‐guided haemorrhoidal artery ligation: long‐term outcome and patient satisfaction
Authors: Wilkerson P. M. , Strbac M. , Reece‐Smith H. , Middleton S. B. 
Published: June 2008

AbstractObjective  Conventional Milligan–Morgan haemorrhoidectomy is associated with significant pain and potentially hazardous complications. Doppler‐Guided Haemorrhoidal Artery Ligation (DGHAL) may offer a lower risk, pain‐free alternative. We present our early and long‐term outcome experience with DGHAL, combined with patient views and satisfaction with the procedure.Method  One hundred and thirteen DGHALs were performed over a 13 month period by two surgeons in a single centre. Patients graded the severity of postoperative pain on visual‐analogue scales. Clinical follow‐up was at 6 weeks (n = 103), with long‐term follow‐up (n = 90) by postal questionnaire at median of 30 months.Results  Seven out of one hundred and three (6%) patients reported postoperative discomfort requiring analgesia. Ninety‐three out of one hundred and three (90%) patients reported complete relief or significant improvement in their symptoms at 6 weeks, dropping to 77/90 (86%) at 30 months. Anal fissures developed in 2/103 (2%) patients, both treated with Diltiazem ointment. Further surgery was required in 8/90 (9%) patients. Eighty‐two out of ninety (91%) patients said they would undergo DGHAL again.Conclusion  DGHAL is a relatively painless, safe, and effective procedure for symptomatic stage I–III haemorrhoids, for which we have demonstrated long‐term durability and acceptability. Its role lies between office based procedures and more invasive operative interventions.
REFLECTIONS ON TERRITORIAL COHESION AND EUROPEAN SPATIAL PLANNING
Authors: EVERS DAVID 
Published: June 2008

ABSTRACTFrom a strictly legal point of view, the European Union does not have authority to carry out spatial planning. The closest thing resembling a spatial policy is the outdated and unofficial European Spatial Development Perspective (ESDP). The inclusion of the term ‘territorial cohesion’ in the Reform Treaty has created new opportunities for proponents of European spatial planning. Despite wide political support for the concept, there is still little consensus on its actual meaning. The purpose of this paper is to take stock of this curious situation. This is done by raising some fundamental issues. Does a ‘planning subject’ and ‘planning object’ exist in Europe? What different interpretations of territorial cohesion are there, and what implications could this have? These issues are investigated further by means of a recent ESPON study which produced several policy‐oriented spatial scenarios. Ultimately, the lack of a precise definition of territorial cohesion may prove to be advantageous.
THE ABDUCTION OF EUROPE: A PLEA FOR LESS ‘UNIONISM’ AND MORE EUROPE
Authors: BOEDELTJE FREERK , VAN HOUTUM HENK 
Published: June 2008

ABSTRACTThis article claims that the development of a European super–state is undesirable and unreal. Europe has never presented itself as a single bordered entity supported by a constitution and a clear definition of Europe and non‐Europe. The contemporary forceful abduction by the European Union re‐creates Europe as a bounded political entity institutionalised through treaties and acts. It is made a socio‐political cultural construction embedded in its own created geography, history and culture. We will argue that European Union is not the same as Europe and that Europe is not a people. Europe is neither finite nor restrictive. The invention and fabrication of a bounded version of Europe leads to new forms of ‘spatial containerisation’ which carries the name of ‘Europeanization’.
Formation of clinopyroxene + spinel and amphibole + spinel symplectites in coronitic gabbros from the Sierra de San Luis (Argentina): a key to post‐magmatic evolution
Authors: CRUCIANI G. , FRANCESCHELLI M. , GROPPO C. , BROGIONI N. , VASELLI O. 
Published: June 2008

AbstractThe El Arenal metagabbros preserve coronitic shells of orthopyroxene ± Fe‐oxide around olivine, as well as three different types of symplectite consisting of amphibole + spinel, clinopyroxene + spinel and, more rarely, orthopyroxene + spinel. The textural features of the metagabbros can be explained by the breakdown of the olivine + plagioclase pair, producing orthopyroxene coronas and clinopyroxene + spinel symplectites, followed by the formation of amphibole + spinel symplectites, reflecting a decrease in temperature and, possibly, an increase in water activity with respect to the previous stage. The metagabbros underwent a complex P–T history consisting of an igneous stage followed by cooling in granulite, amphibolite and greenschist facies conditions. Although the P–T conditions of emplacement of the igneous protolith are still doubtful, the magmatic assemblage suggests that igneous crystallization occurred at a pressure lower than 6 kbar and at 900–1100 °C. Granulitic P–T conditions have been estimated at about 900 °C and 7–8 kbar combining conventional thermobarometry and pseudosection analysis. Pseudosection calculation has also shown that the formation of the amphibole + spinel symplectite could have been favoured by an increase in water activity during the amphibolite stage, as the temperature of formation of this symplectite strongly depends on aH2O (<740 °C for aH2O = 0.5; <790 °C for aH2O = 1). Furthermore, but not pervasive, re‐equilibration under greenschist facies P–T conditions is documented by retrograde epidote and chlorite. The resulting counterclockwise P–T path consists of progressive, nearly isobaric cooling from the igneous stage down to the granulite, amphibolite and greenschist stage.
New polymorphic dinucleotide and trinucleotide microsatellite loci for hop Humulus lupulus L.
Authors: JAKSE J. , LUTHAR Z. , JAVORNIK B. 
Published: June 2008

AbstractOne hundred and thirty‐five microsatellite markers were developed for hop Humulus lupulus L. from di‐ and trinucleotide‐enriched libraries. Seventy‐eight primers showed amplification in two tested genotypes. Twenty‐four loci were further characterized on a population of 34 hop samples and the number of alleles per locus, observed heterozygosity and expected heterozygosity ranged from two to 20 (9.7 on average), from 0.0294 to 0.9412 (0.6234 on average) and from 0.0294 to 0.9170 (0.6720 on average), respectively. These microsatellite markers will be further used for studying population structures and relationships and for identifying important qualitative and quantitative loci of hop.
Development of cytochrome b primers for mitotyping of barbels (Barbus spp.)
Authors: GANTE H. F. , ALVES M. J. , DOWLING T. E. 
Published: June 2008

AbstractPrimers were developed that allow for rapid, reliable and inexpensive screening of cytochrome b by analysis of single‐stranded conformational polymorphisms. Twenty different haplotypes were identified from six species of Iberian Barbus. These primers proved useful for population and species level studies, and could also be valuable in population genetic and phylogenetic studies of other cyprinin fishes.
Accurate methods of DNA extraction and PCR‐based genotyping for single scallop embryos/larvae long preserved in ethanol
Authors: ZHAN AIBIN , BAO ZHENMIN , HU XIAOLI , LU WEI , WANG SHI , PENG WEI , WANG MINGLING , HUI MIN , HU JINGJIE 
Published: June 2008

AbstractMarine scallops are sessile as adult but have a long planktonic larval phase showing great possibility to migrate in marine realm lacking of obvious barriers. Genetic analysis of scallop embryos/larvae based on molecular markers is very essential to clarify the spatial and temporal gene flow and the unique population and community structure. However, the technical challenges, such as single embryos/larvae isolation and low quantity and poor quality of DNA extracted, make genotyping for a single embryo/larva long preserved in ethanol to be a really difficult task. In this study, we analysed the factors that might affect the DNA quantity and quality for simple sequence repeat‐based genotyping for single embryos/larvae. Based on the factors analysed, we developed a LoTEPA buffer‐based method, of which the accuracy, stability and reproducibility were evaluated by controlled inter‐ and intraspecies and self‐fertilized scallop families. The genotyping results showed the high success rate of more than 90% in total for embryos/larvae preserved in ethanol for 1–5 years. Furthermore, the successful genotyping for the larvae sampled from a natural habitat well demonstrated the potential use of this method in practical ecological analysis.
SIMIL: an r (CRAN) scripts collection for computing genetic structure similarities based on structure 2 outputs
Authors: NADIR ALVAREZ , NILS ARRIGO , CONSORTIUM INTRABIODIV 
Published: June 2008

AbstractThe emergence of comparative phylogeography requires tools that allow comparing quantitatively the genetic structures between species. Whereas numerous methods have been developed to compare trees inferred from two species, comparison methods involving population structures issued from Bayesian inferences or maximum likelihood criterion have been poorly investigated. Here, we present a method implemented in an r (CRAN) scripts collection, SIMIL, based on the mean absolute differences computed from structure 2 outputs. The scripts collection is illustrated by the computation of unweighted and weighted genetic‐structure‐similarity (GSS) indices in three alpine plants. Different weighting procedures — taking into account the level of overlap between the species sampling areas — are compared among the different species pairs and among the different numbers of gene pools considered in structure.
Isolation of compound microsatellite loci in the herbaceous perennial Cirsium purpuratum (Maxim.) Matsum.
Authors: WU BINGYUN , SHIOKAWA AKIKO , MATSUSHITA NORIHISA , HOGETSU TAIZO 
Published: June 2008

AbstractThe herbaceous perennial Cirsium purpuratum is a pioneer on the southeast side of Mount Fuji in Japan. For genetic analysis of reproduction in this species, we developed polymorphic compound microsatellite markers using an adaptor‐ligated library method and a simpler method called the intercompound microsatellite method. The latter method was an effective method for developing compound simple sequence repeat markers. In total, 11 polymorphic, codominant microsatellite markers were developed and characterized for this species. These polymorphic markers had three to 20 alleles per locus, a range of observed heterozygosity from 0.25 to 0.90, and were considered effective for genetic analysis.
Characterization of 12 highly variable tetranucleotide microsatellite loci for Arctic grayling (Thymallus arcticus) and cross amplification in other Thymallus species
Authors: DIGGS MATTHEW D. , ARDREN WILLIAM R. 
Published: June 2008

AbstractWe describe the isolation and development of 12 polymorphic tetranucleotide microsatellite loci for Arctic grayling (Thymallus arcticus). In a sample of 46 fish, we observed between three and 20 alleles per locus. All 12 of these loci were also polymorphic in at least one of the following Thymallus species and subspecies: T. burejensis, T. amurensis, T. thymallus, T. brevirostris, T. grubii, T. arcticus baicalensis and T. arcticus pallasi. These loci will aid in our understanding of the population genetics, behaviour and conservation of grayling species throughout the northern hemisphere.