bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Analysis of Dermatology-Related Red-Light Therapy Patient Questions on Reddit
Authors: Wang Jennifer Y. , Patel Paras , Bitterman David , Jagdeo Jared 
Published: March 2024

BACKGROUND
Reddit is one of the world's most popular social media platforms and is increasingly used as a health information resource for patients on topics such as red-light (RL) therapy.

OBJECTIVE
In this article, the authors present an analysis of prevalent patient questions and concerns regarding RL therapy.

METHODS
All posts on the “Hot” page of the r/redlighttherapy subreddit were analyzed and categorized.

RESULTS
A total of 930 questions from 664 posts were analyzed. The most commonly asked question category was related to product recommendations or feedback (29.7%), followed by usage instructions (15.3%), safety and side effects (12.6%), and indications and efficacy (12.3%).

CONCLUSION
Understanding patient concerns and questions about RL, as expressed on online platforms like Reddit, can help clinicians improve patient satisfaction, education, and clinical outcomes. The study offers an innovative approach by using social media to uncover valuable patient insights that might not be easily observable within clinical settings.
Cosmetic Contact Lenses in the United Kingdom: Assessment of Online Regulation and Quality of Consumer Health Information
Authors: Ng Benjamin , Azzopardi Matthew , Ditchfield Alice , Zi Jin , Logeswaran Abison , Hawthorne Imogen , Ting Darren S. J. , Chong Yu Jeat 
Published: March 2024

Objectives:
To determine the compliance of online vendors to the UK Opticians Act 1989 Section 27 requirements and safety regulations for cosmetic contact lens (CCL) sales and the quality of online CCL health information.

Methods:
The top 50 websites selling CCLs on each three search engines, namely Google, Yahoo, and Bing, were selected. Duplicates were removed, and the remaining websites were systematically analyzed in February 2023. UK legal authorization for CCL sales was assessed using the Opticians Act Section 27 and safety regulations determined by the presence of Conformité Européene (CE) marking. The quality and reliability of online information was graded using the DISCERN (16–80) and JAMA (0–4) scores by two independent reviewers.

Results:
Forty-seven eligible websites were analyzed. Only six (12.7%) met the UK legal authorization for CCL sales. Forty-nine different brands of CCLs were sold on these websites, of which 13 (26.5%) had no CE marking. The mean DISCERN and JAMA benchmark scores were 26 ± 12.2 and 1.3 ± 0.6, respectively (intraclass correlation scores: 0.99 for both).

Conclusions:
A significant number of websites provide consumers with easy, unsafe, and unregulated access to CCLs. Most online stores do not meet the requirements set out in the Opticians Act for CCL sales in the United Kingdom. A significant number of CCLs lack CE marking, while the average quality of information on websites selling CCLs is poor. Together, these pose a risk to consumers purchasing CCLs from unregulated websites, and therefore, further stringent regulations on the online sales of these products are needed.
“Window of Opportunity” in Ocular Graft-Versus-Host Disease Treatment: Results of a Longitudinal Study and Case Reports
Authors: Surenkhuu Bayasgalan , Mun Christine S. , Kim Christian , Atassi Nour Yanna , Mun Jessica , Dhall Nikhil , Abdel-Hadi Sarah , Sheth Tanya , Dondeti Priyanka , Bernal Alexandria , Pradeep Anubhav , Rondelli Damiano , Jain Sandeep 
Published: March 2024

Objective:
To perform a longitudinal study for determining the development of ocular graft-versus-host disease (oGVHD) after allogeneic hematopoietic stem cell transplant (HSCT) and report cases that illustrate the “window of opportunity” concept in oGVHD treatment.

Methods:
Patients (n=61) were examined at prescheduled clinic visits before HSCT and three-month intervals after HSCT for 2 years. The presence or absence of oGVHD was determined using the international chronic oGVHD consensus group diagnostic criteria. Ocular surface washings (OSW) were obtained at each visit and analyzed for cytokine levels.

Results:
In the longitudinal study, 26.2% (n=16; progressed group) developed either probable (11.5%, n=7) or definite oGVHD (14.8%, n=9). In the progressed group, clinically significant changes in signs (corneal staining and Schirmer I test) and symptoms at the post-HSCT visit as compared with the pre-HSCT visit occurred at 9 months. Significant differences in clinical signs and symptoms (whether average post-HSCT values or changes in values over pre-HSCT levels) between the progressed and nonprogressed groups occurred at a 9-month visit or later. In the progressed group, 55.6% of eyes that had negative matrix metalloproteinase 9 (MMP-9) test at pre-HSCT turned MMP-9 positive at 3 to 6 months post-HSCT. In the progressed group, interleukin 8 levels in OSW were significantly increased at 6 months post-HSCT. In the case reports, the “window of opportunity” was detected by MMP-9 turning positive, early corneal staining, interleukin 8 increase in OSW, and peripheral corneal epithelial thinning, which resolved with treatment initiation.

Conclusions:
A “window of opportunity” exists before patients developing symptomatic tear-deficient dry eye after HSCT for initiating treatment that may preempt oGVHD development; however, larger-scale longitudinal studies are needed for definitive recommendations.
Frequency, clinical, and laboratory findings of platelet secretion disorders in patients referred to the specialized coagulation laboratory of the Iranian Blood Transfusion Organization
Authors: Shahbazi Massoumeh , Ahmadinejad Minoo , Mahabadi Vahid Pirhajati , Teimourpour Amir , Golzadeh Khadijeh 
Published: March 2024

Objectives
Platelet secretion disorders (PSDs) are a subgroup of platelet function disorders (PFDs) caused by defects in the content or release of platelet granules. These patients have a variable degree of mucocutaneous bleeding tendency. The diagnostic facilities of PSDs are imitated in Iran, even in specialized coagulation laboratories. The present study aims to estimate the frequency of PSDs among patients referred to the Iranian Blood Transfusion Organization (IBTO).

Methods
The research population includes all patients referred to the specialized coagulation laboratory of IBTO and requested platelet function and von Willebrand testing by their physicians. They were recruited between May 2022 and October 2022 if they were not diagnosed as having procoagulant defects, von Willebrand disease (VWD), Glanzmann thrombasthenia (GT), Bernard-Soulier syndrome (BSS), and platelet count <100 × 109 (except in the syndromic forms). Patients with a defect in response to at least two agonists in Light transmission aggregometry (LTA), one agonist in the ATP-secretion study, and/or impairment in the expression of CD62P are considered PSDs.

Results
Among 121 cases referred to our center over 6 months, 40 patients fulfilled the inclusion and exclusion criteria. Ten patients were diagnosed with PSDs. Six were classified as δ-platelet secretion disorders (δ-PSD), two α-platelet secretion disorders (α-PSD), and two αδ-platelet secretion disorders (αδ-PSD).

Conclusions
The prevalence of PSDs in our population study was 25% (10/40), which seems highly prevalent. Therefore, expanding laboratory approaches to platelet function defects is necessary as a routine in our country.
Levodopa–Carbidopa–Entacapone Intestinal Gel in Advanced Parkinson Disease: A Multicenter Real-Life Experience
Authors: Szász József Attila , Dulamea Adriana Octaviana , Constantin Viorelia Adelina , Mureşanu Dafin Fior , Dumbravă Lăcrămioara Perju , Tiu Cristina , Jianu Dragoş Cătălin , Simu Mihaela , Ene Amalia , Axelerad Any , Falup-Pecurariu Cristian , Lungu Mihaela , Danci Adina Gabriela , Sabau Monica , Strilciuc ştefan , Popescu Bogdan Ovidiu 
Published: March 2024

Background:
For Parkinson disease (PD) patients who have been diagnosed with advanced disease that can no longer be effectively controlled with optimized oral or transdermal medications, a range of device-aided therapies (DAT) are available, comprising either deep brain stimulation or infusion therapies providing continuous dopaminergic stimulation. Levodopa–entacapone–carbidopa intestinal gel (LECIG) infusion is the latest DAT for advanced PD (APD) that was approved in Romania in 2021.

Study Question:
What is the experience to date in real-world clinical practice in Romania regarding the efficacy and tolerability of LECIG in APD?

Study Design:
A retrospective evaluation of 74 APD patients treated with LECIG at 12 specialized APD centers in Romania.

Measures and Outcomes:
Demographic data and various clinical parameters were recorded, including Mini Mental State Evaluation score or Montreal Cognitive Assessment Test score. Levodopa-equivalent daily dose and the administered doses of levodopa and other PD medications were evaluated at baseline and after starting LECIG treatment. The efficacy of LECIG in reducing daily hours of off time, motor fluctuations, and dyskinesias were assessed. Any percutaneous endoscopic gastrojejunostomy system or device complications after starting LECIG treatment were noted.

Results:
At baseline, patients were taking oral levodopa for a mean of 5.3 times per day, with a high proportion also taking concomitant add-on therapies (dopamine agonists, 86%, monoamine oxidase type-B inhibitors, 53%; catechol-O-methyltransferase inhibitors, 64%). LECIG treatment significantly reduced daily off time versus baseline from 5.7 h/d to 1.7 hours per day (P < 0.01). Duration and severity of dyskinesias was also significantly reduced versus baseline, and improvements were observed in Hoehn and Yahr Scale scores. LECIG treatment also allowed a significant reduction in the use of concomitant oral medications.

Conclusions:
These findings suggest that LECIG treatment is an effective DAT option in APD that can simplify the treatment regimen.
Improved Short-Term Prognosis of Pediatric Partial-Thickness Burns
Authors: Liu Hailiang , Li Dongjie , Yuan Huageng , Sun Tianjun , Li PengCheng , Cai Zhiwei , Shen Chuan'an 
Published: March 2024

ObjectivesEarly wound management for pediatric patients with partial-thickness burns in the emergency department remains debatable. This study aims to evaluate the value of emergency conservative debridement under topical anesthesia in improving short-term prognosis of pediatric partial-thickness burns.MethodsThis retrospective cohort study enrolled children with partial-thickness thermal burns presenting to the emergency department within 6 hours postburn. All the enrolled patients were divided into 2 groups: the debridement group and the dressing group. The associations between emergency conservative debridement and time to reepithelialization was analyzed by using Kaplan-Meier curves with log rank test and multivariate Cox regression analysis. Moreover, the associations between emergency conservative debridement and in-hospital cost and length of stay were also evaluated.ResultsAll baseline characteristics between groups were comparable (allP> 0.05). Emergency conservative debridement under topical anesthesia significantly decreased the median value of time to reepithelialization (13 vs 14 days,P= 0.02). Cox regression analysis showed that emergency conservative debridement significantly improved wound reepithelialization after adjusting for burn size (odds ratio, 4.07; 95% confidence interval, 1.64–10.11;P< 0.01). The mean length of stay of patients receiving conservative wound debridement was lower than that of patients in the wound dressing group (14.3 ± 7.3 vs 18.8 ± 10.4 days,P< 0.01), but not in terms of mean in-hospital cost per 1% total body surface area (2.8 ± 1.9 vs 3.0 ± 2.1 × 103 RMB per 1% total body surface area,P= 0.58).ConclusionsEmergency conservative debridement of pediatric partial-thickness burns under topical anesthesia significantly improves the wound healing outcomes without increasing health care burden.
Insects visitFusarium xyrophilumpseudoflowers on the hostXyris surinamensis(Xyridaceae) and carry fungal DNA on their bodies
Authors: Torres-Cruz Terry J. , Cofer Tristan M. , Kaminsky Laura M. , Ré Lauren A. , Johnson Jack R. , Vaughn Alexis , Tumlinson James H. , Laraba Imane , Proctor Robert H. , Kim Hye-Seon , Bell Terrence H. , Aime M. Catherine , Skvarla Michael J. , Geiser David M. 
Published: March 2024

AbstractThe fungusFusarium xyrophilumproduces flower-like structures (i.e., pseudoflowers) that were recently discovered on yellow-eyed grasses (Xyrisspp.) in Guyana. It is unknown whether these pseudoflowers, which are composed entirely of fungal tissue, are true mimics that attract insects as a means of fungal dispersal. We evaluated the potential ofF. xyrophilumto affect insect visitation patterns to flowers and pseudoflowers by 1) documenting insect visitation toX. surinamensisin Guyana, 2) measuring the presence ofF. xyrophilumDNA on insects, and 3) evaluating fluorescence and volatile production on flowers and pseudoflowers. We report for the first time Vespidae, Formicidae, Salticidae, Acrididae, and Tetrigidae visitingXyris. Diverse insects, including Conocephalini spp. (meadow katydids; Tettigoniidae),Camponotusspp. (carpenter ants; Formicidae), and a Geometridae sp. (geometer moths) were found to visit flowers and pseudoflowers.Fusarium xyrophilumDNA was detected on 3/12 (25%) of captured insect bodies using conventional and quantitative PCR. Volatiles produced in the field by pseudoflowers and flowers were similar, except for the presence of a sesquiterpene, putatively identified here as α-gurjunene, which was detected both inF. xyrophilumpure cultures and field-collected pseudoflower samples, but not from flowers. The production of this sesquiterpene byF. xyrophilumand the fluorescence ofX. surinamensispeduncles represent potential signals involved in insect attraction for this system. These observations, along with the overlap in insect visitors of flowers and pseudoflowers and the detection ofF. xyrophilumDNA on insect bodies, are consistent with insect visitors being vectors ofXyrispollen andF. xyrophilumpropagules between host plants.
A new type of AmpC β-lactamases defined by PIB-1, a metal-dependent carbapenem-hydrolyzing β-lactamase, fromPseudomonas aeruginosa: structural and functional analysis
Authors: Medrano Francisco Javier , Hernando-Amado Sara , Martínez José Luis , Romero Antonio 
Published: March 2024

AbstractAntibiotic resistance is one of most important health concerns nowadays. β-lactamases are the most important resistance determinants. Based on their structural and functional characteristics β-lactamases are grouped in four categories. AmpC β-lactamases are cephalosporinases presenting a set of highly conserved residues. Here we crystallized PIB-1, aPseudomonas aeruginosachromosomally-encoded β-lactamase. Its crystal structure shows it is an AmpC β-lactamase, although the number of conserved residues is low. Functional analysis showed that PIB-1 is able to degrade carbapenems but not the typical substrate of AmpC β-lactamases, cephalosporins. Besides, the catalytic activity of PIB-1 increases in the presence of metal ions. Metals do not bind to the active center and increase the degradation of the antibiotic. They induce the formation of trimers. This suggests that the oligomer is more active than the monomer. While PIB-1 is structurally an AmpC β-lactamase, the low sequence conservation, substrate profile and its metal-dependence, prompts us to position this enzyme as the founder of a new group inside the AmpC β-lactamases. Consequently, the diversity of AmpC β-lactamases might be wider than expected.
HyperGen: Compact and Efficient Genome Sketching using Hyperdimensional Vectors
Authors: Xu Weihong , Hsu Po-Kai , Moshiri Niema , Yu Shimeng , Rosing Tajana 
Published: March 2024

AbstractMotivationGenomic distance estimation is a critical workload since exact computation for whole-genome similarity metrics such as Average Nucleotide Identity (ANI) incurs prohibitive runtime overhead. Genome sketching is a fast and memory-efficient solution to estimate ANI similarity by distilling representativek-mers from the original sequences. In this work, we present HyperGen that improves accuracy, runtime performance, and memory efficiency for large-scale ANI estimation. Unlike existing genome sketching algorithms that convert large genome files into discretek-mer hashes, HyperGen leverages the emerging hyperdimensional computing (HDC) to encode genomes into quasi-orthogonal vectors (Hypervector, HV) in high-dimensional space. HV is compact and can preserve more information, allowing for accurate ANI estimation while reducing required sketch sizes. In particular, the HV sketch representation in HyperGen allows efficient ANI estimation using vector multiplication, which naturally benefits from highly optimized general matrix multiply (GEMM) routines. As a result, HyperGen enables the efficient sketching and ANI estimation for massive genome collections.ResultsWe evaluate HyperGen’s sketching and database search performance using several genome datasets at various scales. HyperGen is able to achieve comparable or superior ANI estimation error and linearity compared to other sketch-based counterparts. The measurement results show that HyperGen is one of the fastest tools for both genome sketching and database search. Meanwhile, HyperGen produces memory-efficient sketch files while ensuring high ANI estimation accuracy.AvailabilityA Rust implementation of HyperGen is freely available under the MIT license as an open-source software project athttps://github.com/wh-xu/Hyper-Gen. The scripts to reproduce the experimental results can be accessed athttps://github.com/wh-xu/experiment-hyper-gen.Contactwexu@ucsd.edu
Development of a new genotype–phenotype linked antibody screening system
Authors: Watanabe Takashi , Hata Hikaru , Mochizuki Yoshiki , Yokoyama Fumie , Hasegawa Tomoko , Kumar Naveen , Kurosaki Tomohiro , Ohara Osamu , Fukuyama Hidehiro 
Published: March 2024

AbstractAntibodies are powerful tools for the therapy and diagnosis of various diseases. In addition to conventional hybridoma-based screening, recombinant antibody-based screening has become a common choice; however, its application is hampered by two factors: 1) screening starts after Ig gene cloning and recombinant antibody production only, and 2) the antibody is composed of paired chains, heavy and light, commonly expressed by two independent expression vectors. Here, we introduce a method for the rapid screening of recombinant monoclonal antibodies by establishing a Golden Gate-based dual-expression vector andin-vivoexpression of membrane-bound antibodies. Using this system, we demonstrate the rapid isolation of influenza cross-reactive antibodies with high affinity from immunized mice within 7 days. This system is particularly useful for isolating therapeutic or diagnostic antibodies, e.g., during foreseen pandemics.Impact StatementA Golden Gate-based dual-expression vector enables rapid screening, which facilitates efficient isolation of high-affinity cross-reactive antibodies for therapeutic or diagnostic use and provides a crucial advance for pandemic preparedness.
Overexpression of barley heat stress transcription factor HvHsfA6a provide thermotolerance by thermopriming
Authors: Chaudhary Reeku , Baliyan Suchi , Sirohi Parul , Singh Sweta , Mishra Sumit Kumar , Rajkumar Mohan Singh , Saini Shashank Sagar , Germain Hugo , Sircar Debabrata , Chauhan Harsh 
Published: March 2024

AbstractAdverse impacts of climate change, including high temperature on cereal crop production, have been evidenced globally. In plants, heat shock factors (HSFs) are crucial components of heat stress associated rescue mechanisms and are also required for normal biological processes. Here, we functionally characterized a highly heat stress responsiveHvHSFA6ain barley by developing constitutively overexpressing transgenic lines. These transgenic lines showed heat tolerant phenotype via improved photosynthesis, antioxidants and upregulation of HSPs and metabolites involved in stress amelioration and keeping thermomemory as compared to wild type plants. Global transcriptomics and ChIP sequencing revealed that HvHSFA6a orchestrates the expression of several genes through direct binding with other HSFs containing consensus HSE in their promoter regions. A GC-MS based metabolomics analysis also revealed the alterations in key metabolic processes such as carbohydrate metabolism, citric acid cycle, amino acids and secondary metabolism. Higher accumulation of key metabolites such as sucrose, galactinol, shikimate and ascorbate has been observed under both control and heat stress in transgenic lines as compared to wild type plants. Taken together, the results suggest that overexpression ofHvHsfA6aprime the plants for heat stress conditions by alteration in gene expression and metabolic status.HighlightPriming is a mechanism by which plants respond to various abiotic and biotic stresses. Through multi omics approach we found that barley HsfA6a provide thermotolernce in transgenic plants through priming effect on transcriptome and metabolome.
Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory function
Authors: Ma Xiangyu , Chen Li-Nan , Liao Menghui , Zhang Liyan , Xi Kun , Guo Jiamin , Shen Cangsong , Shen Dan-Dan , Cai Pengjun , Shen Qingya , Qi Jieyu , Zhang Huibing , Zang Shao-Kun , Dong Ying-Jun , Miao Luwei , Qin Jiao , Ji Su-Yu , Li Yue , Liu Jianfeng , Mao Chunyou , Zhang Yan , Chai Renjie 
Published: March 2024

AbstractThe class C orphan GPCR GPR156, which lacks the typical extracellular region, plays a pivotal role in auditory function through Gi2/3. Here, we demonstrate that GPR156 with high constitutive activity is essential for maintaining auditory function, and we further present two cryo-EM structures of human GPR156. The GPR156 dimer in both the apo state and Gi3protein-coupled state adopt a TM5/6-TM5/6 interface, indicating the high constitutive activity of GPR156 in the apo state. The C-terminus plays a dual role in promoting G protein binding within G-bound subunit while preventing the G-free subunit from binding to additional G protein. These observations explain how GPCR activity is maintained through dimerization and provide a mechanistic insight into the sustained role of GPR156 in maintaining auditory function.