bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caspase 8 deletion causes infection/inflammation-induced bone marrow failure and MDS-like disease in mice
Authors: Liu Shanhui , Joshi Kanak , Zhang Lei , Li Wenyan , Mack Ryan , Runde Austin , Hagen Patrick A. , Barton Kevin , Breslin Peter , Ji Hong-Long , Kini Ameet R. , Wang Zhiping , Zhang Jiwang 
Published: April 2024

AbstractMyelodysplastic syndromes (MDS) are a heterogeneous group of pre-leukemic hematopoietic disorders characterized by cytopenia in peripheral blood due to ineffective hematopoiesis and normo- or hypercellularity and morphologic dysplasia in bone marrow (BM). An inflammatory BM microenvironment and programmed cell death of hematopoietic stem/progenitor cells (HSPCs) are thought to be the major causes of ineffective hematopoiesis in MDS. Pyroptosis, apoptosis and necroptosis (collectively, PANoptosis) are observed in BM tissues of MDS patients, suggesting an important role of PANoptosis in MDS pathogenesis. Caspase 8 (Casp8) is a master regulator of PANoptosis, which is downregulated in HSPCs from most MDS patients and abnormally spliced in HSPCs from MDS patients with SRSF2 mutation. To study the role of PANoptosis in hematopoiesis, we generated inducible Casp8 knockout mice (Casp8−/−). Mx1-Cre-Casp8−/− mice died of BM failure within 10 days of polyI:C injections due to depletion of HSPCs. Rosa-ERT2Cre-Casp8−/− mice are healthy without significant changes in BM hematopoiesis within the first 1.5 months after Casp8 deletion. Such mice developed BM failure upon infection or low dose polyI:C/LPS injections due to the hypersensitivity of Casp8−/− HSPCs to infection or inflammation-induced necroptosis which can be prevented by Ripk3 deletion. However, impaired self-renewal capacity of Casp8−/− HSPCs cannot be rescued by Ripk3 deletion due to activation of Ripk1-Tbk1 signaling. Most importantly, mice transplanted with Casp8−/− BM cells developed MDS-like disease within 4 months of transplantation as demonstrated by anemia, thrombocytopenia and myelodysplasia. Our study suggests an essential role for a balance in Casp8, Ripk3-Mlkl and Ripk1-Tbk1 activities in the regulation of survival and self-renewal of HSPCs, the disruption of which induces inflammation and BM failure, resulting in MDS-like disease.
Communication spectrum prediction method based on convolutional gated recurrent unit network
Authors: Yuan Lige , Nie Lulu , Hao Yangzhou 
Published: April 2024

AbstractIn modern wireless communication systems, the scarcity of spectrum resources poses challenges to the performance and efficiency of the system. Spectrum prediction technology can help systems better plan and schedule resources to respond to the dynamic changes in spectrum. Dynamic change in the spectrum refers to the changes in the radio spectrum in a wireless communication system. It means that the available spectrum resources may change at different times and locations. In response to this current situation, this study first constructs a communication collaborative spectrum sensing model using channel aliasing dense connection networks. Then, combining convolutional neural network and gated cyclic unit network in deep learning technology, a communication spectrum prediction model is built. It aims to achieve accurate perception and prediction of spectrum resources through the aforementioned spectrum sensing and prediction models. The results confirm that the proposed perception model has inconsistent perception accuracy under different number of secondary users, with a maximum of 0.99. It is verified that the proposed spectrum prediction model achieves a high prediction accuracy of 0.95 within 208 s and its performance outperforms current similar models. The results are based on the model's deep learning analysis of massive historical communication data, in which the optimized shuffle dense net model plus convolutional gated recurrent unit model is the key to achieve fast and accurate prediction. On the contrary, the highest spectrum prediction accuracy of Recurrent Neural Network (RNN), Long Short-Term Memory (LSTM), and Convolutional Neural Networks-Long Short-Term Memory (ConvLSTM) models are 0.86, 0.90, and 0.85, respectively. And the model needs to run for a longer period of time, up to 324, for ConvLSTM to reach the prediction accuracy value of 0.95. In summary, the perception and prediction model built by this research has good performance, and its application in the field of wireless communication can assist staff in better monitoring spectral changes, thereby making more efficient use of spectral resources.
Assessment of bioremediation potential of Calotropis procera and Nerium oleander for sustainable management of vehicular released metals in roadside soils
Authors: Anjum Sumreen , Sarwar Mubeen , Ali Qurban , Alam Muhammad Waqar , Manzoor Muhammad Tariq , Mukhtar Adnan 
Published: April 2024

AbstractLand transportation is a major source of heavy metal contamination along the roadside, posing significant risks to human health through inhalation, oral ingestion, and dermal contact. Therefore, this study has been designed to determine the concentrations of vehicular released heavy metals (Cd, Pb, Ni, and Cu) in roadside soil and leaves of two commonly growing native plant species (Calotropis procera and Nerium oleander).Two busy roads i.e., Lahore-Okara road (N-5) and Okara-Faisalabad roads (OFR) in Punjab, Pakistan, were selected for the study. The data were collected from five sites along each road during four seasons. Control samples were collected ~ 50 m away from road. The metal content i.e. lead (Pb), cadmium (Cd) nickel (Ni) and copper (Cu) were determined in the plant leaves and soil by using Atomic Absorption Spectrophotometer (AAS). Significantly high amount of all studied heavy metals were observed in soil and plant leaves along both roads in contrast to control ones. The mean concentration of metals in soil ranged as Cd (2.20–6.83 mg/kg), Pb (4.53–15.29 mg/kg), Ni (29.78–101.26 mg/kg), and Cu (61.68–138.46 mg/kg) and in plant leaves Cd (0.093–0.53 mg/kg), Pb (4.31–16.34 mg/kg), Ni (4.13–16.34 mg/kg) and Cu (2.98–32.74 mg/kg). Among roads, higher metal contamination was noted along N-5 road. Significant temporal variations were also noted in metal contamination along both roads. The order of metal contamination in soil and plant leaves in different seasons was summer > autumn > spring > winter. Furthermore, the metal accumulation potential of Calotropis procera was higher than that of Nerium oleander. Therefore, for sustainable management of metal contamination, the plantation of Calotropis procera is recommended along roadsides.
Development and validation of the rheumatoid arthritis scale among the system of quality of life instruments for chronic diseases QLICD-RA (V2.0)
Authors: Yang Zheng , Bai Guannan , Ding Haifeng , Chen Mingyang , Xie Tong , Wan Chonghua 
Published: April 2024

AbstractRheumatoid Arthritis is a more serious threatening to people and suitable for QOL measurement. A few specific QOL instruments are available without considering Chinese culture. The present study was aimed to develop and validate the Rheumatoid Arthritis Scale among the System of Quality of Life Instruments for Chronic Diseases (QLICD-RA V2.0). The data collected from 379 patients with RA was used to evaluate the psychometric properties of the scale. The reliability was evaluated by the internal consistency Cronbach’s α, test–retest reliability Pearson correlation r and intra-class correlation (ICC). We evaluated the construct validity and criteria-related validity by correlation analysis and structural equation modeling. We compared the differences in scores of QLICD-RA before and after treatment and used the Standard Response Mean (SRM) to assess the responsiveness. The results showed that the internal consistency coefficient Cronbach’s α values were greater than 0.70. The correlations r and ICCs were greater than 0.80. The correlation analysis and structural equation modeling confirmed good construct validity and criterion-related validity. The SRM ranges from 0.07 to 0.27 for significant domains/facets. It concluded that QLICD-RA (2.0) is a reliable and valid instrument to measure QOL among patients with RA.
Ultrasonographic examination of masticatory muscles in patients with TMJ arthralgia and headache attributed to temporomandibular disorders
Authors: Lee Yeon-Hee , Bae Hyungkyu , Chun Yang-Hyun , Lee Jung-Woo , Kim Hee-Jin 
Published: April 2024

AbstractThis study used ultrasonography to compare the thickness and cross-sectional area of the masticatory muscles in patients with temporomandibular joint arthralgia and investigated the differences according to sex and the co-occurrence of headache attributed to temporomandibular disorders (HATMD). The observational study comprised 100 consecutive patients with TMJ arthralgia (71 females and 29 males; mean age, 40.01 ± 17.67 years) divided into two groups: Group 1, including 86 patients with arthralgia alone (60 females; 41.15 ± 17.65 years); and Group 2, including 14 patients with concurrent arthralgia and HATMD (11 females; 33.00 ± 16.72 years). The diagnosis of TMJ arthralgia was based on the diagnostic criteria for temporomandibular disorders. The parameters of the masticatory muscles examined by ultrasonography were subjected to statistical analysis. The pain area (2.23 ± 1.75 vs. 5.79 ± 2.39, p-value = 0.002) and visual analog scale (VAS) score (3.41 ± 1.82 vs. 5.57 ± 12.14, p-value = 0.002) were significantly higher in Group 2 than in Group 1. Muscle thickness (12.58 ± 4.24 mm) and cross-sectional area (4.46 ± 2.57 cm2) were larger in the masseter muscle than in the other three masticatory muscles (p-value < 0.001). When examining sex-based differences, the thickness and area of the masseter and lower temporalis muscles were significantly larger in males (all p-value < 0.05). The area of the masseter muscle (4.67 ± 2.69 vs. 3.18 ± 0.92, p-value = 0.004) and lower temporalis muscle (3.76 ± 0.95 vs. 3.21 ± 1.02, p-value = 0.049) was significantly smaller in Group 2 than in Group 1. An increase in VAS was significantly negatively correlated with the thickness of the masseter (r =  − 0.268) and lower temporalis (r =  − 0.215), and the cross-sectional area of the masseter (r =  − 0.329) and lower temporalis (r =  − 0.293). The masseter and lower temporalis muscles were significantly thinner in females than in males, and their volumes were smaller in patients with TMJ arthralgia and HATMD than in those with TMJ arthralgia alone. HATMD and decreased masseter and lower temporalis muscle volume were associated with increased pain intensity.
Route planning of mobile robot based on improved RRT star and TEB algorithm
Authors: Yin Xiong , Dong Wentao , Wang Xiaoming , Yu Yongxiang , Yao Daojin 
Published: April 2024

AbstractThis paper presents a fusion algorithm based on the enhanced RRT* TEB algorithm. The enhanced RRT* algorithm is utilized for generating an optimal global path. Firstly, proposing an adaptive sampling function and extending node bias to accelerate global path generation and mitigate local optimality. Secondly, eliminating path redundancy to minimize path length. Thirdly, imposing constraints on the turning angle of the path to enhance path smoothness. Conducting kinematic modeling of the mobile robot and optimizing the TEB algorithm to align the trajectory with the mobile robot's kinematics. The integration of these two algorithms culminates in the development of a fusion algorithm. Simulation and experimental results demonstrate that, in contrast to the traditional RRT* algorithm, the enhanced RRT* algorithm achieves a 5.8% reduction in path length and a 62.5% decrease in the number of turning points. Utilizing the fusion algorithm for path planning, the mobile robot generates a superior, seamlessly smooth global path, adept at circumventing obstacles. Furthermore, the local trajectory meticulously conforms to the kinematic constraints of the mobile robot.
Effects of a beaver dam on the benthic copepod assemblage of a Mediterranean river
Authors: Di Lorenzo T. , Tabilio Di Camillo A. , Mori E. , Viviano A. , Mazza G. , Pontalti A. , Rogora M. , Fiasca B. , Di Cicco M. , Galassi D. M. P. 
Published: April 2024

AbstractAs known “ecosystem engineers”, beavers influence river hydrology, geomorphology, biochemistry, and biological assemblages. However, there is a lack of research regarding the effects of beaver activities on freshwater meiofauna. In this study, we investigated the taxonomic and functional composition of the benthic copepod assemblage of a segment of the Tiber River (Italy) where a beaver dam, created about 7 weeks before our survey, had formed a semi-lentic habitat upstream and a lotic habitat downstream of the dam. We also analyzed the copepod assemblage before and after a flood event that destroyed the beaver dam, providing a unique opportunity to observe changes in a naturally reversing scenario. Our analyses revealed that, while the taxonomic composition and functional traits of the copepod assemblage remained largely unchanged across the recently formed semi-lentic and lotic habitats, substantial differences were evident between the dammed and undammed states. The dammed state showed lower copepod abundances, biomass, and functionality than the undammed one. These results highlight the role of beaver dams in changing the composition and functionality of meiofaunal assemblages offering insights into the dynamic interactions within aquatic ecosystems.
Quantum mechanical modeling of high-intensity laser pulse interaction with hydrogen atom with considering the magnetic field and polarization
Authors: Zakavi Marjan , Sabaeian Mohammad 
Published: April 2024

AbstractIn the study of the non-relativistic interaction between high-intensity femtosecond laser pulses and atoms, the influence of the magnetic field is commonly overlooked. This work investigates the effects of the magnetic field in the high-intensity few-cycle laser pulses with non-relativistic intensity of $$3.5 \times 10^{14} { }\;\;{\text{W}}/{\text{cm}}^{2}$$

3.5
×

10
14

W
/

cm

2

at the center wavelength of 800 nm on the high-order harmonic generation (HHG), attosecond pulse train (APT), isolated attosecond pulse (IAP), and the electron trajectory in the hydrogen atom, employing the numerical solution of the time-dependent Schrödinger equation in three dimensions (3D-TDSE). Two polarizations, linear and circular, are considered. A comparison with the scenario where the magnetic field is not considered shows that the magnetic field can apply significant corrections to the results. Particularly, considering the magnetic field for circular polarization can make the cutoff frequency of HHG coincide with the semi-classical relationship of $$\hbar {\upomega }_{{\text{c}}} = {\text{I}}_{{\text{p}}} + 3.17{\text{U}}_{{\text{p}}}$$

ħ

ω
c

=

I
p

+
3.17

U
p

, a case that for circular polarization does not exist without considering the magnetic field. Moreover, accounting for the magnetic field leads to a reduction in the attosecond pulse duration for circular polarization for APT ($$360{\text{ as}}$$

360

as

versus $$241{\text{ as}}$$

241

as

) and for IAP ($$834{\text{ as}}$$

834

as

versus $$602{\text{ as}}$$

602

as

). Additionally, the difference in production efficiency of HHG and APT between linear and circular polarization is reduced by two orders of magnitude, when magnetic field is considered. Although considering the magnetic field complicates the electron trajectory, especially for circular polarization, however, our quantum model provides enhanced insight into how the interaction works, especially when and where the electron collides with the parent nucleus. In this case, the quantum mechanical modeling largely covers the huge difference of not considering the magnetic field in the results predicted by other works.
Soluble programmed death ligand 1 as prognostic biomarker in non-small cell lung cancer patients receiving nivolumab, pembrolizumab or atezolizumab therapy
Authors: Brun Sinne Søberg , Hansen Torben Frøstrup , Wen Sara Witting Christensen , Nyhus Christa Haugaard , Bertelsen Lisbeth , Jakobsen Anders , Hansen Torben Schjødt , Nederby Line 
Published: April 2024

AbstractMany studies have focused on the prognostic role of soluble programmed death ligand 1 (sPD-L1) in non-small cell lung cancer (NSCLC), but outcomes are ambiguous and further investigations are needed. We addressed the matter by studying sPD-L1 in baseline samples and in longitudinal samples taken prior to three subsequent cycles of anti-PD-1/anti-PD-L1 treatments. Eighty patients with NSCLC were enrolled. Median sPD-L1 level at baseline was 52 pg/mL [95% confidence interval (CI) 49–57]. In patients treated with pembrolizumab and nivolumab, the concentration of sPD-L1 remained rather stable throughout treatment. In contrast, sPD-L1 rose by 50-fold following the first cycle of atezolizumab therapy. We found the baseline level of sPD-L1 to be related to overall survival (OS) after two years of follow-up in simple Cox analysis (p = 0.006) and multiple Cox Regression, hazard ratio 1.02 (95% CI 1.00–1.03) (p = 0.033). There was no association between sPD-L1 and tissue PD-L1 expression, overall response rate, or progression free survival. In conclusion, sPD-L1 measured in baseline serum samples may be associated with OS in NSCLC patients receiving anti-PD1/anti-PD-L1 treatment. Importantly, the results signify that further research is warranted to explore the clinical utility of sPD-L1 in patients treated with anti-PD-L1.
Design of a recombinant asparaginyl ligase for site-specific modification using efficient recognition and nucleophile motifs
Authors: Tang Jiabao , Hao Mengling , Liu Junxian , Chen Yaling , Wufuer Gulimire , Zhu Jie , Zhang Xuejie , Zheng Tingquan , Fang Mujin , Zhang Shiyin , Li Tingdong , Ge Shengxiang , Zhang Jun , Xia Ningshao 
Published: April 2024

AbstractAsparaginyl ligases have been extensively utilized as valuable tools for site-specific bioconjugation or surface-modification. However, the application is hindered by the laborious and poorly reproducible preparation processes, unstable activity and ambiguous substrate requirements. To address these limitations, this study employed a structure-based rational approach to obtain a high-yield and high-activity protein ligase called OaAEP1-C247A-aa55-351. It was observed that OaAEP1-C247A-aa55-351 exhibits appreciable catalytic activities across a wide pH range, and the addition of the Fe3+ metal ion effectively enhances the catalytic power. Importantly, this study provides insight into the recognition and nucleophile peptide profiles of OaAEP1-C247A-aa55-351. The ligase demonstrates a higher recognition ability for the “Asn-Ala-Leu” motif and an N-terminus “Arg-Leu” as nucleophiles, which significantly increases the reaction yield. Consequently, the catalytic activity of OaAEP1-C247A-aa55-351 with highly efficient recognition and nucleophile motif, “Asn-Ala-Leu” and “Arg-Leu” under the buffer containing Fe3+ is 70-fold and 2-fold higher than previously reported OaAEP1-C247A and the most efficient butelase-1, respectively. Thus, the designed OaAEP1-C247A-aa55-351, with its highly efficient recognition and alternative nucleophile options, holds promising potential for applications in protein engineering, chemo-enzymatic modification, and the development of drugs.
Raman fingerprint provides deep insights into alternations in Ganoderma lingzhi triterpene-induced molecular events in cells
Authors: Shen Shuwei , Huang Binbin , Peng Zhiyi , Yu Luting , Ren Lili , Liu Jia , Chen Guoguang 
Published: April 2024

Precisely monitoring the alternations of biomolecules in traditional Chinese medicine-induced cells via a novel Raman fingerprint based analytical strategy.
Functionalized Schiff-base containing phenothiazine and cholic acid as a paper-based fluorescence turn-off sensor for Sn(ii) ion detection and its application
Authors: Bariya Dipakkumar , Mishra Satyendra 
Published: April 2024

The development of the cholyl hydrazide Schiff base of phenothiazine as a paper-based sensor for the detection of Sn(ii) ions with chelation-enhanced fluorescence quenching sensing mechanism and its possible integration into a logic gate system.
Burst traffic: Congestion management and performance optimization strategies in heterogeneous cognitive radio networks
Authors: Yan Jie , Zhang Bo , Wang Shi , Lan Hao , Wang Xin 
Published: April 2024

AbstractIn order to solve the network congestion problem caused by burst service flows in heterogeneous cognitive radio networks (Het‐CRN), a two‐stage dual‐threshold random early detection (TD‐RED) control mechanism is proposed here. Meanwhile, a three‐state Markov modulated Poisson process (MMPP‐3) is used to model the burst service to analyze its arrival in depth. Moreover, a performance evaluation system (PES) is proposed to quantify the interference of burst services to different classes of services and their quality of service, which can comprehensively evaluate the performance enhancement of the TD‐RED control mechanism for each service. The system examines performance metrics such as packet loss rate, average delay, average captain etc. PES simulation results show that TD‐RED significantly reduces the packet rejection rate and transmission delay compared to the no congestion control (NCC) and random early detection (RED) methods, thus providing superior quality of service to all services. thereby providing a superior quality of service to cognitive users. Its lower average captain fully proves that TD‐RED can effectively alleviate congestion.
Hybrid nonlinear ripple‐free sampled‐data robust regulation with application to UAVs
Authors: Larios‐Navarro Ulises , García‐Vázquez Horacio , Castillo‐Toledo Bernardino , Gennaro Stefano Di 
Published: April 2024

AbstractThis paper proposes a novel solution to the problem of robust ripple‐free hybrid robust regulator ensuring the elimination of intersample ripples. The paper considers a structurally stable regulation approach in the hybrid framework. A regulator is designed using this technique under periodic sampling and is applied to the nonlinear model of a quadrotor. The control of unmanned autonomous vehicles has become a research topic that is important by itself, particularly in the case of multi‐rotor systems due to their versatility in various applications. Tracking a reference signal for such vehicles can be challenging, particularly when the frequency of communication is limited, or the control station is located far away. The proposed controller is hence tested for this challenging application.