bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and characterization of microsatellite markers for the waratah, Telopea speciosissima (Proteaceae)
Authors: PORTER CAROLYN , RYMER PAUL D. , ROSSETTO MAURIZIO 
Published: March 2006

AbstractThirteen microsatellite loci were developed from an (AG)‐enriched library using Telopea speciosissima, a widespread species of dry‐sclerophyll forest in New South Wales (NSW, Australia). Genotyping of 30 seeds from one population of T. speciosissima and 15 seeds from one population of the related Telopea aspera (restricted to the Gibraltar Range in northern NSW) was successfully performed. The loci were also tested across seven other related species. The microsatellites will be used to compare population dynamics across a range of taxa representing different distribution patterns.
Development and testing of novel chloroplast microsatellite markers for Lolium perenne and other grasses (Poaceae) from de novo sequencing and in silico sequences
Authors: MCGRATH S. , HODKINSON T. R. , SALAMIN N. , BARTH S. 
Published: March 2006

AbstractTwelve primers to amplify microsatellite markers from the chloroplast genome of Lolium perenne were designed and optimized using de novo sequencing and in silico sequences. With one exception, each locus was polymorphic with a range from two to nine alleles in L. perenne. The newly developed primer pairs cross‐amplified in different species of Lolium and in 50 other grass species representing nine grass subfamilies.
Molecular markers for systematic identification and population genetics of the invasive Ponto‐Caspian freshwater gammarid Dikerogammarus villosus (Crustacea, Amphipoda)
Authors: WATTIER R. A. , BEGUET J. , GAILLARD M. , MÜLLER J. C. , BOLLACHE L. , PERROT‐MINNOT M.‐J. 
Published: March 2006

AbstractThe Ponto‐Caspian amphipod, Dikerogammarus villosus, is an invasive species of many European rivers. First, we show that size difference of nrDNA ITS1 allows discriminating D. villosus from Dikerogammarus bispinosus, a closely related but morphologically hardly distinguishable species. Second, we present two types of polymorphic markers for D. villosus, three microsatellites and two single nucleotide polymorphisms (SNPs) of mtDNA COI gene, which were scored by polymerase chain reaction‐single strand conformational polymorphism (PCR‐SSCP). These markers will be very useful in studying population genetics of D. villosus.
Isolation and characterization of novel microsatellite loci for the short‐beaked common dolphin (Delphinus delphis) and cross‐amplification in other cetacean species
Authors: COUGHLAN J. , MIRIMIN L. , DILLANE E. , ROGAN E. , CROSS T. F. 
Published: March 2006

AbstractSeven tetranucleotide and three dinucleotide microsatellite loci were isolated from the short‐beaked common dolphin (Delphinus delphis). Number of alleles and observed heterozygosity ranged from three to twelve (mean 8.4) and from 0.087 to 0.978 (mean 0.741), respectively. All loci were tested in four other odontocete species and showed polymorphisms in most locus–species combinations. This panel of nuclear markers will be useful for the investigation of population ecology and social interactions in common dolphins and other cetacean species.
Characterization of polymorphic microsatellite loci within a young Boophilus microplus metapopulation
Authors: KOFFI BROU BASILE , RISTERUCCI ANGE MARIE , JOULIA DOMINIQUE , DURAND PATRICK , BARRÉ NICOLAS , DE MEEÛS THIERRY , CHEVILLON CHRISTINE 
Published: March 2006

AbstractNine microsatellite loci from the cattle tick Boophilus microplus were isolated and characterized within New Caledonia. This Pacific island was invaded by the cattle tick from a few immigration events dating from mid‐20th century. A population survey involving 94 adult ticks indicated monomorphism at one locus, presence of null alleles at another, and high genetic diversity (0.61–0.72) at seven loci apparently suitable for population genetics studies. This opens the opportunity to dissect the populational mechanisms involved in the spectacular capacity of B. microplus to cope with local environmental heterogeneity during its recurrent invasions of tropical areas.
Eight new polymorphic microsatellite loci for genetic analyses in the endangered common hamster (Cricetus cricetus L.)
Authors: JAKOB SABINE S. , MAMMEN KERSTIN 
Published: March 2006

AbstractWe developed primers for eight polymorphic microsatellite loci in the endangered common hamster (Cricetus cricetus L.). Population genetic parameters were analysed on the basis of 70 adult individuals captured from a single population. Allele numbers per locus ranged from eight to 17. Expected and observed heterozygosities per locus ranged from 0.67 to 0.91 and from 0.41 to 0.8, respectively.
Characterization of microsatellite loci for the redbanded seabream, Pagrus auriga (Teleostei, Sparidae)
Authors: PONCE MARIAN , INFANTE CARLOS , CRESPO ANIELA , ZUASTI EUGENIA , PÉREZ LAURA , FUNES VICTORIA , CATANESE GAETANO , CÁRDENAS SALVADOR , MANCHADO MANUEL 
Published: March 2006

AbstractThe redbanded seabream Pagrus auriga (Teleostei, Sparidae) is a species of a high commercial value in Spain. There is currently little information available about the genetic characteristics of both wild and cultured populations. In this survey, we have developed eight polymorphic microsatellites for the redbanded seabream using an enriched genome library protocol. All of them were polymorphic in the 64 individuals tested, 22 of which were wild specimens, and 42 were individuals from a captive reproductive broodstock. These markers can potentially be useful tools for use in population genetic studies.
Eight polymorphic microsatellite loci for the aquatic fungus Tetracladium marchalianum
Authors: ANDERSON J. L. , BEEVER J. , SHEARER C. A. 
Published: March 2006

AbstractWe have identified eight polymorphic microsatellite loci from the aquatic haploid fungus Tetracladium marchalianum. Microsatellite polymorphism was evaluated using 90 fungal isolates, each of single spore origin, from submerged leaves in Illinois and Wisconsin rivers in the USA. The number of alleles observed for a single locus ranged from three to 13, with up to 10 observed from a population. These genetic markers will be useful to evaluate population structure, genotypic diversity, and the reproductive mode of T. marchalianum.
Characterization of 19 single nucleotide polymorphism markers for coho salmon
Authors: SMITH C. T. , PARK L. , VANDOORNIK D. , SEEB L. W. , SEEB J. E. 
Published: March 2006

AbstractWe report 39 single nucleotide polymorphisms (SNPs) observed in 23 nuclear DNA sequences in coho salmon Oncorhynchus kisutch. High‐throughput genotyping assays based on the 5′‐nuclease reaction were developed for 17 of these nuclear SNPs and for two previously published mitochondrial DNA SNPs. Minor allele frequency differences (Δq) among collections were between 5.2% and 51.2%, resulting in per locus FST estimates of 0.00–0.24 with an average of 0.09.
Eight microsatellite markers for the San Esteban chuckwalla, Sauromalus varius
Authors: MCALILEY L. REX , WILLIS RAYMOND E. , FORSTNER MICHAEL R. J. , GUERRA TRINA , DENSMORE LLEWELLYN D. 
Published: March 2006

AbstractWe characterize eight polymorphic microsatellite loci for the San Esteban chuckwalla, Sauromalus varius, which are being used as markers in a study of the genetic results from a decade of captive breeding in this endangered species. Observed heterozygosity estimates for S. varius ranged from 0.00 to 0.30, whereas expected heterozygosity estimates ranged from 0.06 to 0.58.
Polymorphic microsatellite DNA markers in the mudflat topshell Diloma subrostrata (Gastropoda, Trochidae)
Authors: DONALD K. M. , SPENCER H. G. 
Published: March 2006

AbstractVery little information is available on the intraspecific genetic structure of topshell populations. Here, we report the characterization of five polymorphic microsatellite loci in the New Zealand mudflat topshell, Diloma subrostrata. The number of alleles per locus ranged from two to 23, observed and expected heterozygosities did not deviate from Hardy–Weinberg equilibrium (P < 0.05) and no linkage disequilibrium was detected between locus pairs (P < 0.05). We are currently using these markers to investigate genetic population structure of D. subrostrata in New Zealand.
Use of an indirect effect model to describe the LDL cholesterol‐lowering effect by statins in hypercholesterolaemic patients
Authors: Faltaos Demiana William , Urien Saïk , Carreau Valérie , Chauvenet Marina , Hulot Jean Sebastian , Giral Philippe , Bruckert Eric , Lechat Philippe 
Published: March 2006

AbstractStatins are the most commonly prescribed agents for the treatment of hypercholesterolaemia. This is due to their efficacy in reducing low‐density lipoprotein cholesterol (LDL) level which is the primary goal of the treatment especially for patients with multiple risk factors or with established coronary heart diseases. The purpose of this study was to develop a pharmacokinetic/pharmacodynamic (PK/PD) model that describes the LDL‐lowering process in patients with hypercholesterolaemia treated with atorvastatin, fluvastatin or simvastatin. A total of 100 patients were studied retrospectively. They received atorvastatin (n = 57), fluvastatin (n = 26) or simvastatin (n = 17). As no pharmacokinetic data were available, the absorption rate was fixed to 1/h and atorvastatin, simvastatin and fluvastatin elimination half‐lives were fixed to 14, 2 and 2.5 h respectively. A total of 309 LDL levels were measured and the data were analysed by nonmem v. The time course of the LDL‐lowering effect of statins was described by an indirect‐response model with precursor (LDL synthesis, input rate Kin) and response (circulating LDL, input and output rates K) compartments. The following parameters were estimated: LDL input rate (Kin) 0.14 ± 0.015 g/L/day (mean ± SD); inhibition fraction of Kin (INH) 0.21 ± 0.017; and dose producing 50% increase of LDL removal (D50), 26 ± 7.8, 1.3 ± 0.48 and 15 ± 5.25 mg for atorvastatin, simvastatin and fluvastatin, respectively. Gender, bodyweight, age, calories/day, sugar/day, lipids/day, hyperlipidaemia types and waist/hip circumference, renal and hepatic functions had no effect on the pharmacodynamic parameters. The pharmacodynamic parameters for the three statins were accurately estimated. The PK/PD model developed successfully predicted the time course of the LDL‐lowering effect of statins.
Effect of acute inhibition of nitric oxide synthesis byl‐NAME on cardiovascular responses following peripheral autonomic blockade in rabbits
Authors: Eldesoky Ehab S. 
Published: March 2006

AbstractThe pressor and chronotropic responses to acute inhibition of nitric oxide synthase enzyme byNG‐nitro‐l‐arginine methyl ester (l‐NAME) were studied in anaesthetized rabbits with intact autonomic nervous system (ANS) activity. Also, they were investigated when administration ofl‐NAME was preceded by peripheral autonomic blockade. Autonomic blockade had different forms: ganglionic (hexamethonium‐induced), post‐ganglionicβ‐adrenergic blockade (propranolol induced), parasympathetic blockade (atropine induced), and complete autonomic blockade by coadministration of hexamethonium and atropine simultaneously.l‐NAME injected intravenously (10 mg/kg) in animals with intact and blocked autonomic activity induced a pressor response. This pressor response was accompanied by bradycardia in rabbits with either intact autonomic activity or hexamethonium‐induced ganglionic blockade.l‐NAME exerted no effect on heart rate in animals withβ‐adrenergic blockade or parasympathetic blockade. In rabbits with complete autonomic blockade,l‐NAME evoked tachycardia. These experiments indicate thatl‐NAME‐induced hypertension is not relying only on ANS. Also,l‐NAME‐induced tachycardia in rabbits treated with atropine plus hexamethonium suggests other humoral mechanisms that may be involved in thel‐NAME induced chronotropic response.
Birds in willow short‐rotation coppice compared to other arable crops in central England and a review of bird census data from energy crops in the UK
Authors: SAGE RUFUS , CUNNINGHAM MARK , BOATMAN NIGEL 
Published: March 2006

We present the results of spring and winter surveys of birds using 22 willow short‐rotation coppice (SRC) fields and 22 paired arable or grassland controls over a 5 year period in central and northern England. The study sites were linked to the first commercial wood‐fuelled electricity plant in Yorkshire and were managed commercially. We use the findings of this study, in the context of other work on birds in energy crops, to suggest how birds may be affected by commercial cropping of SRC or energy grasses on UK farmland. For willow SRC, most existing farmland species were not completely displaced by SRC cropping. Even farmland specialists such as Skylark Alauda arvensis used cut SRC fields. Some species characteristic of scrub and wood‐type habitats were attracted onto farmland by SRC. Birds were more abundant in hedgerows next to SRC than in those adjoining arable or grass. The interior of large SRC plots contained fewer birds than the edge‐zone (< 50 m). In winter, the same resident species occured in SRC as the summer, plus winter migrants such as Snipe Gallinago gallinago and Fieldfare Turdus pilaris. Overall, more individuals and species were recorded in and around SRC than equivalent arable or grassland throughout the year. Yellow Wagtail Motacilla flava and certain locally occurring farmland specialists may be displaced by SRC plantations. Equivalent census data for energy grasses in the UK are not available. The impact of large regional energy crop developments on birds is not known. We do not know if species commonly recorded in these crops survive and breed well so population effects are unknown. However SRC crops are often weedy and insect rich and hence have potential as foraging habitats in summer and winter. The scope for agri‐environment schemes to include energy crops is discussed.
Measuring stair use in two office buildings: a comparison between an objective and a self‐reported method
Authors: Engbers L. H. , Van Poppel M. N. M. , Van Mechelen W. 
Published: March 2006

Introduction/purpose: Measuring stair use reliably and objectively is complicated and difficult. In this study, stair use was measured at an individual level by using an innovative registration system and was compared with self‐reported data. The purpose of this study was to gain an insight into the comparability of self‐reported stair use vs objectively measured stair use.Methods: Self‐reported and objective stair use was measured in two worksites and was operationalized as how often a subject uses the stairs per week (i.e., stair‐use frequency) and the number of floors covered (up or down) in a week with each use. Analyses were performed by means of the intraclass correlation coefficients (ICCs).Results: A number of significant differences in stair use between worksites were found. ICCs of 0.55 and 0.24 for stair‐use frequency were found in worksites 1 and 2, respectively. The ICCs for the number of floors covered were lower at 0.39 and 0.19 for worksites 1 and 2, respectively.Conclusion: The comparability of self‐reported and objectively measured stair use is moderate to poor, and given the independent measurement errors of both methods, this might have been expected. Comparability seemed to be dependent on worksite characteristics.
Strain rate and tissue tracking imaging in quantitation of left ventricular systolic function in endurance and strength athletes
Authors: Poulsen S. H. , Hjortshøj S. , Korup E. , Poenitz V. , Espersen G. , Søgaard P. , Suder P. , Egeblad H. , Kristensen B. Ø. 
Published: March 2006

Aim: The aim of this study was to assess the impact of long‐term physical training on left ventricular longitudinal contraction by strain rate analysis and tissue tracking imaging.Methods and results: The study population comprised 17 male elite endurance and 15 male elite strength athletes and 12 male control subjects of similar age. Tissue Doppler imaging was recorded in the apical views and used for analysis of the longitudinal systolic myocardial velocity, annular diastolic velocities, strain rate and tissue tracking. Left ventricular mass index was significantly increased in both endurance athletes (209±40 g/m2) and strength athletes (138±38 g/m2) compared with normal subjects (96±20 g/m2, P<0.001). Tissue tracking score index and mean strain rate of the 16 segments were significantly increased in strength athletes (7.9±1.1 mm and –1.4±0.3 s−1, respectively) compared with endurance athletes (7.5±0.9 mm and –1.0±0.4 s−1, P<0.01 for both) and normal subjects (7.4±1.0 mm and −1.0±0.3 s−1, P<0.01 for both).Conclusion: Despite significant left ventricular hypertrophy and extensive training in elite athletes, we found normal longitudinal left ventricular systolic function, and in strength athletes performing isometric exercise even increased function.
Physical fitness profile of elite athletes with intellectual disability
Authors: Van de Vliet P. , Rintala P. , Fröjd K. , Verellen J. , Van Houtte S. , Daly D. J. , Vanlandewijck Y. C. 
Published: March 2006

The aim of this study was to investigate the physical fitness profile of high‐performance athletes with intellectual disability (ID) in comparison with able‐bodied individuals.Methods: Participants were 231 male and 82 female athletes. All evaluations were done using the EUROFIT physical fitness test.Results: In comparison with population data, both male and female athletes with ID score better for flexibility and upper body muscle endurance, but have similar or lower values for running speed, speed of limb movement, and strength measures. Compared with age‐matched physical education students, male athletes with ID score better for running speed and flexibility, and worse for strength. Female athletes with ID score not different from able‐bodied individuals for flexibility, running speed, and upper body muscle endurance, but worse for strength measures. Athletes with ID also have poorer cardio respiratory endurance capacity compared with sportive peers without ID. Furthermore, male athletes have a more differentiated profile depending upon their sports discipline, compared with female athletes.Conclusion: It can be concluded that high‐performance athletes with ID reach physical fitness levels that are equal to or lower than those of able‐bodied sportive counterparts. Further research should investigate the importance of reduced muscle strength to be the limiting factor.
Effects of Emdogain on osteoblast gene expression
Authors: Carinci F , Piattelli A , Guida L , Perrotti V , Laino G , Oliva A , Annunziata M , Palmieri A , Pezzetti F 
Published: March 2006

Objective:  Emdogain (EMD) is a protein extract purified from porcine enamel and has been introduced in clinical practice to obtain periodontal regeneration. EMD is composed mainly of amelogenins (90%), while the remaining 10% is composed of non‐amelogenin enamel matrix proteins such as enamelins, tuftelin, amelin and ameloblastin. Enamel matrix proteins seem to be involved in root formation. EMD has been reported to promote proliferation, migration, adhesion and differentiation of cells associated with healing periodontal tissues in vivo.Design:  How this protein acts on osteoblasts is poorly understood. We therefore attempted to address this question by using a microarray technique to identify genes that are differently regulated in osteoblasts exposed to enamel matrix proteins.Results:  By using DNA microarrays containing 20 000 genes, we identified several upregulated and downregulated genes in the osteoblast‐like cell line (MG‐63) cultured with enamel matrix proteins (Emd). The differentially expressed genes cover a broad range of functional activities: (i) signaling transduction, (ii) transcription, (iii) translation, (iv) cell cycle regulation, proliferation and apoptosis, (v) immune system, (vi) vesicular transport and lysosome activity, and (vii) cytoskeleton, cell adhesion and extracellular matrix production.Conclusions:  The data reported are the first genome‐wide scan of the effect of enamel matrix proteins on osteoblast‐like cells. These results can contribute to our understanding of the molecular mechanisms of bone regeneration and as a model for comparing other materials with similar clinical effects.
Cheilitis as a variation of Candida‐associated lesions
Authors: Terai H , Shimahara M 
Published: March 2006

Objective:  Occasionally, the clinical diagnosis of Candida‐associated lesion is difficult because of a variety of its clinical manifestations. There have been a few reports on Candida‐associated lip lesion except angular cheilitis. In this paper, we investigate the relation of Candida in persistent lesions of the lip.Subjects and methods:  The present study includes consecutive nine patients with persistent erosive lip lesion. For each patient the age at presentation, gender, duration of the disease, other symptoms or signs, complete medical history and prior treatment or medications for the symptom were obtained. Then, mycological examination and the direct cytologic examination were performed.Results:  The reported average duration of the disease was 7 months. Six of nine patients had received prior treatments with topical steroids at the other clinic, which failed to resolve their symptoms. Six of nine patients had a predisposing factor for candidiasis. In the culture examination, Candida albicans were isolated in seven patients (77.8%). Five of nine patients received the direct cytologic examination, and four of them revealed pseudohyphae of fungi. The anti‐fungal treatment was miconazol gel 25 mg four times per day, and average duration of the treatment was 2 weeks. The outcome was as follows: complete remission in five, remarkable response in two and no response in two.Conclusion:  Our results suggested that one form of the varieties of Candida‐associated lesions might be considered in the case of lip lesion with unknown origin that was persistent and ineffective to the topical steroids treatment.
Familial tumoral calcinosis and testicular microlithiasis associated with a new mutation of GALNT3 in a white family
Authors: Campagnoli M F , Pucci A , Garelli E , Carando A , Defilippi C , Lala R , Ingrosso G , Dianzani I , Forni M , Ramenghi U 
Published: March 2006

Background:Familial tumoral calcinosis (FTC) is a rare autosomal recessive disease characterised by the development of multiple calcified masses in periarticular soft tissues; GALNT3 gene mutations have recently been described in an African American and in a Druse Arab family with FTC.Objective:To report the clinical and histological features caused by a new GALNT3 mutation in a white family.Results:Homozygosity for the nonsense mutation Lys463X was found in both affected siblings, who displayed a classic phenotype, the male also having testicular microlithiasis. He is the first subject described with testicular microlithiasis in FTC.Conclusions:The high testicular expression of GALNT3 suggests that the gene alteration could act locally by causing deposition of calcium, and the testis may be an underestimated site of calcification in FTC. Autoimmune diseases are present in several members of the family. Although immune disorders have been described in FTC, autoimmunity does not segregate with the GALNT3 mutation in this family.