bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison between 308‐nm monochromatic excimer light and narrowband UVB phototherapy (311–313 nm) in the treatment of vitiligo – a multicentre controlled study
Authors: Casacci M , Thomas P , Pacifico A , Bonnevalle A , Paro Vidolin A , Leone G 
Published: March 2007

AbstractBackground  Vitiligo is an acquired pigmentary disorder characterized by areas of depigmented skin resulting from loss of epidermal melanocytes. Recently, it has been shown that narrowband ultraviolet B (NB‐UVB) phototherapy may be more effective than psoralen and ultraviolet A (PUVA) photochemotherapy in treating vitiligo, and that 308‐nm monochromatic excimer light (MEL) may present some advantages as compared to NB‐UVB for the treatment of vitiligo.Aim  The aim of this study was to compare the effectiveness of NB‐UVB phototherapy and 308‐nm MEL in vitiligo patients.Methods  The study was done in a randomized, investigator‐blinded and half‐side comparison design. Twenty‐one subjects with symmetrical vitiligo lesions were enrolled in this study. Vitiligo lesions on one body side were treated twice weekly for 6 months with 308‐nm MEL, while NB‐UVB phototherapy was used to treat lesions on the opposite side.Results  At the end of the study six lesions (37.5%) treated with 308‐nm MEL and only one lesion (6%) treated with NB‐UVB achieved an excellent repigmentation (score 4) while four lesions (25%) treated with 308‐nm MEL and five lesions (31%) treated with NB‐UVB showed a good repigmentation (score 3).Conclusions  It appears that 308‐nm MEL is more effective than NB‐UVB in treating vitiligo lesions and it induces repigmentation more rapidly.
No significant increase within a 3‐year interval in the prevalence of atopic dermatitis among schoolchildren in Baranya County, Hungary
Authors: Harangi F , Fogarasy A , Müller Á , Schneider I , Sebõk B 
Published: March 2007

AbstractBackground  The prevalence of atopic dermatitis (AD) in children has significantly increased worldwide in the past decades. Although it is well known that the number of AD patients has also been growing in Hungary, there are only a few published prevalence studies that allow international comparisons.Objectives  The aim of this study was to estimate the prevalence of AD among schoolchildren in Baranya County in 2005 and to compare the data with those from 2002.Methods  The data from the 1454 children (771 girls, 683 boys) surveyed in 2002, and 1454 children (760 girls, 694 boys) surveyed in 2005, respectively, aged 7–14 years were analysed. The distinct populations of the 7‐ to 9‐year‐old age groups were separately compared in relation to their lifetime AD prevalence.Results  The prevalence of AD accounted for 15.1% in 2002, and 16.1% in 2005. In the compared distinct 7‐ to 9‐year‐old populations the prevalence rates were 17.0% in 2002 and 17.1% in 2005. There were no statistically significant differences between the data of the two surveys.Conclusions   The results indicate the high prevalence rate of AD nearly approaching the markedly high values registered in the welfare countries, and could indicate that AD has reached a plateau in Hungary.
Polymorphic microsatellite loci isolated from the marine sponge Scopalina lophyropoda (Demospongiae: Halichondrida)
Authors: BLANQUER A. , URIZ M. J. , PASCUAL M. 
Published: March 2007

AbstractA total of seven microsatellites out of 88 isolated from a genomic library enriched for (CA)n and (GA)n repeats were characterized in the Mediterranean marine sponge Scopalina lophyropoda. The microsatellite motifs were large (34.81 ± 13.9 bp) and imperfect. The seven microsatellite loci were screened in 30 individuals collected from Blanes, northwestern Mediterranean. All of them were polymorphic (allele numbers and observed heterozygosities ranged from 3 to 6 and from 0.16 to 0.76, respectively). No significant linkage disequilibrium between pairs of loci and no departure from Hardy–Weinberg equilibrium were found. These markers are therefore promising for studies of the population structure of the species.
Development and characterization of seven novel di‐, tri‐, and tetranucleotide microsatellite markers in Atka mackerel (Pleurogrammus monopterygius)
Authors: SPIES INGRID B. , LOWE SANDRA , HONG YVONNE , CANINO MICHAEL F. 
Published: March 2007

AbstractWe describe the enrichment for and characterization of seven Pleurogrammus monopterygius microsatellite loci. We used (GACA)4 as an oligonucleotide enrichment probe and screened 484 clones from the enriched library. The seven microsatellite loci include five tetranucleotide, one dinucleotide, and one trinucleotide motif. These markers can potentially be useful tools for use in population genetics studies, parentage analyses, and examining other life history questions.
Microsatellite loci from Russula brevipes, a common ectomycorrhizal associate of several tree species in North America
Authors: BERGEMANN S. E. , MILLER S. L. , GARBELOTTO M. 
Published: March 2007

AbstractSix microsatellite loci were isolated and characterized from genomic libraries enriched for (CA)n (GA)n (ATG)n, and (CAG)n, microsatellite motifs from Russula brevipes, a common ectomycorrhizal fungus that forms mutualisms with several species of trees in North America. The polymerase chain reaction primers were tested on 27 sporocarps of R. brevipes sampled in Douglas fir (Pseudotsuga menziesii), grey pine (Pinus sabiniana), and Sitka spruce (Picea sitchensis) stands. The number of alleles per locus ranged from two to 14 with expected heterozygosity values from 0.00 to 0.92 within populations. These are the first six microsatellite loci characterized from Russula brevipes that can be used for estimating genotypic diversity and population structure.
Characterization of polymorphic microsatellite markers for the Carnaby's cockatoo (Calyptorhynchus latirostris) and related black cockatoo species
Authors: McINNES LINDA M. , DADOUR IAN R. , STEWART MEREDITH E. , DITCHAM WILLIAM G. F. , MAWSON PETER , SPENCER PETER B. S. 
Published: March 2007

AbstractMicrosatellite loci were isolated from Carnaby's black cockatoo (Calyptorhynchus latirostris: Aves), a highly valued, endangered, and endemic species of bird from Western Australia. This study describes three dinucleotide and one tetranucleotide microsatellite loci for which the primers produced clear and polymorphic amplification patterns with between two and nine alleles and moderate levels of variability. Two additional dinucleotide markers which were monomorphic in the Carnaby's cockatoo were able to amplify and were polymorphic in two other species of black cockatoo, greatly increasing the utility of these markers.
Microsatellite DNA markers developed for the Australian bass (Macquaria novemaculeata) and their cross‐amplification in estuary perch (Macquaria colonorum)
Authors: SCHWARTZ T. S. , JENKINS F. , BEHEREGARAY L. B. 
Published: March 2007

AbstractThe Australian bass is a catadromous species found in drainages of southeastern Australia. As an economically important resource that is declining in number, the Australian bass is currently extensively stocked in New South Wales and Victoria to meet the requirements of fisheries programs. We have developed six microsatellite markers that amplify in both Australian bass and the congeneric estuary perch. These markers are useful for investigating population genetic structure and for identifying hybrids between these two species.
Development and transportability across Prunus species of 42 polymorphic almond microsatellites
Authors: MNEJJA M. , GARCIA‐MAS J. , HOWAD W. , ARÚS P. 
Published: March 2007

AbstractWe report 47 new simple sequence repeats (SSRs) obtained from a CT/AG enriched genomic library of almond cv. Texas (syn. Mission). Forty‐two of them were polymorphic in a sample of eight almond cultivars and 31 of these were single‐locus. The average values of the number of alleles per locus (6.6), and mean observed (65%) and expected (76%) heterozygosities for these 31 SSRs indicated a high level of variability. All cultivars studied could be individually identified using any one of the five SSRs. Transportability to other Prunus species (peach, sweet cherry, Japanese plum and apricot) was also high (83–100%).
Isolation and characterisation of microsatellite markers for the Australian monitor lizard, Varanus acanthurus (Squamata: Varanidae) and their utility in other selected varanid species
Authors: FITCH A. J. , GOODMAN A. E. , DONNELLAN S. C. 
Published: March 2007

AbstractThere have been no molecular genetic examinations of mating systems in the Australian varanid lizards (genus Varanus) despite their high species diversity, the abundance of some species and difficulties with direct observation of behaviourally cryptic species in the field. We developed 10 polymorphic microsatellite loci and assessed their utility in a range of varanid species. Observed heterozygosities in the three species assessed ranged from 30% to 100%. These loci should be useful for investigation of population structure, gene flow and mating systems in Varanus acanthurus, V. baritji and V. tristis and may also be of use in other varanid species.
Characterization of polymorphic microsatellite markers for the endangered Malagasy radiated tortoise (Geochelone radiata)
Authors: PAQUETTE SEBASTIEN RIOUX , SHORE GARY D. , BEHNCKE SANDRA M. , LAPOINTE FRANÇOIS‐JOSEPH , LOUIS EDWARD E. 
Published: March 2007

AbstractThe radiated tortoise (Geochelone radiata) is an endangered endemic species from Madagascar that inhabits the semiarid spiny forest of the southern part of the island. Habitat destruction and illegal harvesting greatly threaten this species, as attested by the significant reduction of its distribution area in the past 30 years. In order to undertake appropriate conservation actions, it is essential to acquire a better knowledge of its genetic structure. For this study, 145 blood samples were collected from three populations of radiated tortoises in southwestern Madagascar. Eight microsatellite loci were found to be polymorphic, and allelic diversity and observed heterozygosity were high for all markers.
A set of chloroplast microsatellite primers for Eucalyptus (Myrtaceae)
Authors: STEANE D. A. , JONES R. C. , VAILLANCOURT R. E. 
Published: March 2007

AbstractWe present a set of 35 chloroplast microsatellite primers for Eucalyptus. Ten of the microsatellites displayed intraspecific polymorphism, and identified nine haplotypes among 16 Eucalyptus globulus individuals. Primer conservation was high, with a polymerase chain reaction (PCR) success rate of 98% when tested on four other Eucalyptus species and seven additional myrtaceous genera. Chloroplast microsatellites have applications in phylogeographic studies, fingerprinting and progeny analysis.
Characterization of microsatellite loci for the Amazonian rummy‐nose tetra, Hemigrammus bleheri (Teleostei, Characidae)
Authors: BEHEREGARAY L. B. , CHAE J. , CHAO N. L. , CACCONE A. 
Published: March 2007

AbstractThe rummy‐nose tetra, Hemigrammus bleheri, is a small fish found in forest streams of the Rio Negro floodplain, central Amazonia. This species is popular in aquaria and used as an ornamental fishery resource by the riverine people from middle Rio Negro. Here we describe eight microsatellite DNA loci for rummy‐nose tetras. Number of alleles and heterozygosity per locus in a sample of 30 fish ranged from five to 22 and from 0.52 to 0.96, respectively. These highly variable genetic markers provide important tools for investigating population history and identifying conservation units in rummy‐nose tetras.
Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria
Authors: CHAPUIS MARIE‐PIERRE , LOISEAU ANNE , MICHALAKIS YANNIS , LECOQ MICHEL , ESTOUP ARNAUD 
Published: March 2007

AbstractBecause of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross‐taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species.
Development of polymorphic microsatellite markers for the fungal tree pathogen Cryphonectria eucalypti
Authors: NAKABONGE G. , CORTINAS M. N. , ROUX J. , GRYZENHOUT M. , WINGFIELD B. D. , WINGFIELD M. J. 
Published: March 2007

AbstractPolymorphic microsatellite DNA markers were developed from a single spore isolate of Cryphonectria eucalypti collected from a Eucalyptus stem canker in South Africa. Markers were obtained using the enrichment technique known as fast isolation by AFLPs of sequences containing repeats (FIASCO). Ten polymorphic markers were isolated, of which, two were discarded due to their high polymorphism in the flanking region. The mean number of alleles produced by the remaining eight markers from 20 isolates was 7.25, and alleles per locus ranged from four to 12. The markers will be used to study populations of C. eucalypti.
Eleven polymorphic microsatellite loci for the salmonid trematode Plagioporus shawi
Authors: CRISCIONE CHARLES D. , BLOUIN MICHAEL S. 
Published: March 2007

AbstractWe isolated and characterized 11 microsatellite loci from Plagioporus shawi, a parasitic trematode of salmonid fishes (Oncorhynchus spp.). Extensive polymorphism (expected heterozygosities ranging from 0.476 to 0.981 and number of alleles from 10 to 55) was found in a sample of 94 trematodes from 24 infected cutthroat trout (Oncorhynchus clarki) individuals. Nine of the 11 loci will be useful for future genetic studies on within population dynamics of P. shawi.
A method for adjusting allele frequencies in the case of microsatellite allele drop‐out
Authors: BJÖRKLUND MATS 
Published: March 2007

AbstractA new method is proposed to adjust allele frequencies when allelic drop‐out is common. This method assumes Hardy–Weinberg equilibrium (HWE), and treats the problematic alleles as a one‐locus two‐allele system with dominance. By assuming that the homozygote frequency of the ‘recessive’ allele is measured correctly, we can back calculate the allele frequency of the ‘dominant’ allele, and adjust the heterozygote frequency accordingly. The drawback is that multilocus genotypes cannot be constructed and tests that use deviations from Hardy–Weinberg such as tests for bottlenecks become impossible. An example is given where a large homozygote excess (FIS = 0.44) is adjusted to a reasonable level (FIS = 0.046). The effect of scoring error was set in relation to sampling error and while FIS values can be seriously biased, FST values are not necessarily so, if scoring error and sample size are both low. As sample size increases, the effect of scoring error increases.
Characterization of EST derived SSRs from the bay scallop, Argopecten irradians
Authors: ROBERTS STEVEN , ROMANO CHRISTINA , GERLACH GABRIELE 
Published: March 2007

AbstractInterest in bay scallop conservation has resulted in organized stock enhancement efforts and increased attention to fisheries management issues. Genetic markers can facilitate the monitoring of enhancement efforts, characterization of wild populations, and optimize hatchery practices. We have identified eight polymorphic simple sequence repeat markers including one dinucleotide, six trinucleotide and one compound dinucleotide repeats, in expressed sequence tags generated from multiple bay scallop cDNA libraries. The numbers of alleles range from two to five. The expected and observed heterozygosities range from 0.093 to 0.720 and 0.095 to 0.600, respectively.
Characterization of 20 highly variable tetranucleotide microsatellite loci for bull trout (Salvelinus confluentus) and cross‐amplification in other Salvelinus species
Authors: DEHAAN PATRICK W. , ARDREN WILLIAM R. 
Published: March 2007

AbstractWe describe the isolation and development of 20 polymorphic tetranucleotide microsatellite loci for bull trout (Salvelinus confluentus). In a sample of 76 fish, we observed between four and 23 alleles per locus. These loci show greater levels of genetic diversity than other loci previously examined in bull trout. Seventeen of these loci were also polymorphic in at least one of the following species: Salvelinus alpinus, Salvelinus malma, Salvelinus namaycush and Salvelinus fontinalis. These loci will aid in our understanding of the molecular ecology of bull trout and other char.
Microsatellite DNA markers for delineating population structure and kinship among the endangered Kirtland's warbler (Dendroica kirtlandii)
Authors: KING TIM L. , EACKLES MICHAEL S. , HENDERSON ANNE P. , BOCETTI CAROL I. , CURRIE DAVE , WUNDERLE JOSEPH M. 
Published: March 2007

AbstractWe document the isolation and characterization of 23 microsatellite DNA markers for the endangered Kirtland's warbler (Dendroica kirtlandii), a Nearctic/Neotropical migrant passerine. This suite of markers revealed moderate to high levels of allelic diversity (averaging 7.7 alleles per locus) and heterozygosity (averaging 72%). Genotypic frequencies at 22 of 23 (95%) markers conformed to Hardy–Weinberg equilibrium expectations, and no linkage disequilibrium was observed in blood samples taken from 14 warblers found on the wintering grounds in the Bahamas archipelago. Multilocus genotypes resulting from this suite of markers should reduce the amount of resources required for initiating new genetic studies assessing breeding structure, parentage, demographics, and individual‐level ecological interactions for D. kirtlandii.
Characterization of microsatellite loci in the stick insects Bacillus rossius rossius, Bacillus rossius redtenbacheri and Bacillus whitei (Insecta: Phasmatodea)
Authors: ANDERSEN DITTE HOLM , PERTOLDI CINO , LOESCHCKE VOLKER , SCALI VALERIO 
Published: March 2007

AbstractFive microsatellite markers were obtained from a dinucleotide enriched genomic library of the stick insect Bacillus rossius rossius. The markers were tested in three species of Bacillus. All loci were polymorphic when tested across species. The number of alleles at each locus was low (maximum four alleles), but different allelic patters were observed among the species.
Isolation and characterization of microsatellite markers in Calomys musculinus (Muridae, Sigmodontinae, Phyllotini), the natural reservoir of Junin virus
Authors: CHIAPPERO MARINA B. , GARDENAL CRISTINA N. , PANZETTA‐DUTARI GRACIELA M. 
Published: March 2007

Abstract Calomys musculinus, one of the most abundant rodent species in central Argentina, is the reservoir of Junin virus, the aetiological agent of Argentine hemorrhagic fever. We isolated six polymorphic microsatellite loci for microgeographical studies of population structure in this species. Amplification of these loci in 36 individuals from three natural populations revealed five to 14 alleles per locus and expected heterozygosities from 0.426 to 0.868. Cross‐species amplifications suggest that primers designed for these loci may be useful in other closely related species of the tribe Phyllotini, but not in species of other more distantly related tribes of the subfamily Sigmodontinae.
Development of primers to characterize the mitochondrial control region of Galápagos land and marine iguanas (Conolophus and Amblyrhynchus)
Authors: HANLEY T. C. , CACCONE A. 
Published: March 2007

AbstractPrimers were developed for the amplification and sequencing of the mitochondrial control region of Galápagos land (Conolophus) and marine (Amblyrhynchus) iguanas. Sequences were obtained for four land iguana samples from two islands and for 28 marine iguana samples from three islands. A series of 70–80 bp tandem repeats adjacent to the control region are described and preliminary quantification of intra‐ and interspecific sequence divergence is included.
Isolation and characterization of microsatellite loci from the truffle‐like ectomycorrhizal fungi Rhizopogon occidentalis and Rhizopogon vulgaris
Authors: GRUBISHA LISA C. , KRETZER ANNETTE M. , BRUNS THOMAS D. 
Published: March 2007

AbstractWe have isolated and characterized five microsatellite loci from Rhizopogon occidentalis and six loci from Rhizopogon vulgaris (Boletales, Basidiomycota). Microsatellite variation was assessed using 32 R. occidentalis and 48 R. vulgaris individuals from four populations in California. The number of alleles across populations ranged from two to 10 for R. occidentalis and three to eight for R. vulgaris. Expected heterozygosity values within populations ranged from 0.00 to 0.85 for R. occidentalis and 0.00 to 0.75 for R. vulgaris. These are the first microsatellite loci isolated for R. occidentalis and R. vulgaris and will be useful in the examination of their population genetic structure.
Nematode‐specific PCR primers for the 18S small subunit rRNA gene
Authors: FLOYD ROBIN M. , ROGERS ALEX D. , LAMBSHEAD P. JOHN D. , SMITH CRAIG R. 
Published: March 2007

AbstractA set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.
Development and characterization of microsatellite loci in Mormon crickets (Anabrus simplex, Orthoptera, Tettigoniidae)
Authors: BAILEY N. W. , HOCKHAM L. R. , GRAVES J. A. , RITCHIE M. G. 
Published: March 2007

Abstract Anabrus simplex is an economically significant crop pest in the western United States and is a model organism for studying the influence of sex role reversal on sperm allocation and utilization patterns and population genetics. We isolated seven polymorphic microsatellite loci in Anabrus simplex, and within population allele numbers ranged from 10 to 25. High polymorphism is not unusual for Orthopteran insects, although observed heterozygosities ranged from 0.24 to 0.91 and were lower than expected heterozygosities, suggesting null alleles. These microsatellites will greatly facilitate studies of postcopulatory reproductive isolation in nuptial gift‐giving insects and historical phylogenetics in the Rocky Mountains.
Maximum‐likelihood estimation of a hybrid index based on molecular markers
Authors: BUERKLE C. ALEX 
Published: March 2007

AbstractThis paper elaborates on a hybrid index that utilizes information from genetic markers to quantify the genetic contribution of hybridizing species to individuals of unknown ancestry. Dominant markers will only lead to reliable and accurate estimates of hybrid index in later generation hybrids. In contrast, codominant markers can be fully resolved and their use is unproblematic. For both types of markers and allele frequencies that differ substantially between parental species (FST ≥ 0.17), a hybrid index based on 35–45 loci will have a nearly minimal confidence interval. Estimates of hybrid index are robust to modest errors in estimates of parental allele frequencies.