bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multicenter trial of the efficacy and safety of tigecycline versus imipenem/cilastatin in patients with complicated intra-abdominal infections [Study ID Numbers: 3074A1-301-WW; ClinicalTrials.gov Identifier: NCT00081744]
Authors: Oliva María E , Rekha Arcot , Yellin Albert , Pasternak Jacyr , Campos Maria , Rose Gilbert M , Babinchak Timothy , Ellis-Grosse Evelyn J , Loh Evan ,   the 301 Study Group
Published: October 2005

Abstract

Background
Complicated intra-abdominal infections (cIAI) remain challenging to treat because of their polymicrobial etiology including multi-drug resistant bacteria. The efficacy and safety of tigecycline, an expanded broad-spectrum glycylcycline antibiotic, was compared with imipenem/cilastatin (IMI/CIS) in patients with cIAI.

Methods
A prospective, double-blind, multinational trial was conducted in which patients with cIAI randomly received intravenous (IV) tigecycline (100 mg initial dose, then 50 mg every 12 hours [q12h]) or IV IMI/CIS (500/500 mg q6h or adjusted for renal dysfunction) for 5 to14 days. Clinical response at the test-of-cure (TOC) visit (14–35 days after therapy) for microbiologically evaluable (ME) and microbiological modified intent-to-treat (m-mITT) populations were the co-primary efficacy endpoint populations.

Results
A total of 825 patients received ≥ 1 dose of study drug. The primary diagnoses for the ME group were complicated appendicitis (59%), and intestinal (8.8%) and gastric/duodenal perforations (4.6%). For the ME group, clinical cure rates at TOC were 80.6% (199/247) for tigecycline versus 82.4% (210/255) for IMI/CIS (95% CI -8.4, 5.1 for non-inferiority tigecycline versus IMI/CIS). Corresponding clinical cure rates within the m-mITT population were 73.5% (227/309) for tigecycline versus 78.2% (244/312) for IMI/CIS (95% CI -11.0, 2.5). Nausea (31.0% tigecycline, 24.8% IMI/CIS [P = 0.052]), vomiting (25.7% tigecycline, 19.4% IMI/CIS [P = 0.037]), and diarrhea (21.3% tigecycline, 18.9% IMI/CIS [P = 0.435]) were the most frequently reported adverse events.

Conclusion
This study demonstrates that tigecycline is as efficacious as imipenem/cilastatin in the treatment of patients with cIAI.
The burden of diarrhoea, shigellosis, and cholera in North Jakarta, Indonesia: findings from 24 months surveillance
Authors: Agtini Magdarina D , Soeharno Rooswanti , Lesmana Murad , Punjabi Narain H , Simanjuntak Cyrus , Wangsasaputra Ferry , Nurdin Dazwir , Pulungsih Sri Pandam , Rofiq Ainur , Santoso Hari , Pujarwoto H , Sjahrurachman Agus , Sudarmono Pratiwi , von Seidlein Lorenz , Deen Jacqueline L , Ali Mohammad , Lee Hyejon , Kim Deok Ryun , Han Oakpil , Park Jin Kyung , Suwandono Agus , Ingerani  , Oyofo Buhari A , Campbell James R , Beecham H James , Corwin Andrew L , Clemens John D 
Published: October 2005

Abstract

Background
In preparation of vaccines trials to estimate protection against shigellosis and cholera we conducted a two-year community-based surveillance study in an impoverished area of North Jakarta which provided updated information on the disease burden in the area.

Methods
We conducted a two-year community-based surveillance study from August 2001 to July 2003 in an impoverished area of North Jakarta to assess the burden of diarrhoea, shigellosis, and cholera. At participating health care providers, a case report form was completed and stool sample collected from cases presenting with diarrhoea.

Results
Infants had the highest incidences of diarrhoea (759/1 000/year) and cholera (4/1 000/year). Diarrhea incidence was significantly higher in boys under 5 years (387/1 000/year) than girls under 5 years (309/1 000/year; p < 0.001). Children aged 1 to 2 years had the highest incidence of shigellosis (32/1 000/year). Shigella flexneri was the most common Shigella species isolated and 73% to 95% of these isolates were resistant to ampicillin, trimethoprim-sulfamethoxazole, chloramphenicol and tetracycline but remain susceptible to nalidixic acid, ciprofloxacin, and ceftriaxone. We found an overall incidence of cholera of 0.5/1 000/year. Cholera was most common in children, with the highest incidence at 4/1 000/year in those less than 1 year of age. Of the 154 V. cholerae O1 isolates, 89 (58%) were of the El Tor Ogawa serotype and 65 (42%) were El Tor Inaba. Thirty-four percent of patients with cholera were intravenously rehydrated and 22% required hospitalization. V. parahaemolyticus infections were detected sporadically but increased from July 2002 onwards.

Conclusion
Diarrhoea causes a heavy public health burden in Jakarta particularly in young children. The impact of shigellosis is exacerbated by the threat of antimicrobial resistance, whereas that of cholera is aggravated by its severe manifestations.
Cardiac involvement in a patient with clinical and serological evidence of African tick-bite fever
Authors: Bellini Cristina , Monti Matteo , Potin Mathieu , Ave Anne Dalle , Bille Jacques , Greub Gilbert 
Published: October 2005

AbstractBackgroundMyocarditis and pericarditis are rare complications of rickettsiosis, usually associated withRickettsia rickettsiiandR. conorii. African tick-bite fever (ATBF) is generally considered as a benign disease and no cases of myocardial involvement due toRickettsia africae, the agent of ATBF, have yet been described.Case presentationThe patient, that travelled in an endemic area, presented typical inoculation eschars, and a seroconversion againstR. africae, was admitted for chest pains and increased cardiac enzymes in the context of an acute myocarditis.ConclusionOur findings suggest that ATBF, that usually presents a benign course, may be complicated by an acute myocarditis.
Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest
Authors: Lo Hsin-Lung , Nakajima Satoshi , Ma Lisa , Walter Barbara , Yasui Akira , Ethell Douglas W , Owen Laurie B 
Published: October 2005

Abstract

Background
UV-induced damage can induce apoptosis or trigger DNA repair mechanisms. Minor DNA damage is thought to halt the cell cycle to allow effective repair, while more severe damage can induce an apoptotic program. Of the two major types of UV-induced DNA lesions, it has been reported that repair of CPD, but not 6-4PP, abrogates mutation. To address whether the two major forms of UV-induced DNA damage, can induce differential biological effects, NER-deficient cells containing either CPD photolyase or 6-4 PP photolyase were exposed to UV and examined for alterations in cell cycle and apoptosis. In addition, pTpT, a molecular mimic of CPD was tested in vitro and in vivo for the ability to induce cell death and cell cycle alterations.

Methods
NER-deficient XPA cells were stably transfected with CPD-photolyase or 6-4PP photolyase to specifically repair only CPD or only 6-4PP. After 300 J/m2 UVB exposure photoreactivation light (PR, UVA 60 kJ/m2) was provided for photolyase activation and DNA repair. Apoptosis was monitored 24 hours later by flow cytometric analysis of DNA content, using sub-G1 staining to indicate apoptotic cells. To confirm the effects observed with CPD lesions, the molecular mimic of CPD, pTpT, was also tested in vitro and in vivo for its effect on cell cycle and apoptosis.

Results
The specific repair of 6-4PP lesions after UVB exposure resulted in a dramatic reduction in apoptosis. These findings suggested that 6-4PP lesions may be the primary inducer of UVB-induced apoptosis. Repair of CPD lesions (despite their relative abundance in the UV-damaged cell) had little effect on the induction of apoptosis. Supporting these findings, the molecular mimic of CPD, (dinucleotide pTpT) could mimic the effects of UVB on cell cycle arrest, but were ineffective to induce apoptosis.

Conclusion
The primary response of the cell to UV-induced 6-4PP lesions is to trigger an apoptotic program whereas the response of the cell to CPD lesions appears to principally involve cell cycle arrest. These findings suggest that CPD and 6-4 PP may induce differential biological effects in the UV-damaged cell.
HDL enhances oxidation of LDL in vitro in both men and women
Authors: Solakivi T , Jaakkola O , Salomäki A , Peltonen N , Metso S , Lehtimäki T , Jokela H , Nikkari ST 
Published: October 2005

Abstract

Background
Oxidative modification of low-density lipoprotein (LDL) is a key event in the oxidation hypothesis of atherogenesis. Some in vitro experiments have previously suggested that high-density lipoprotein (HDL) co-incubated with LDL prevents Cu2+-induced oxidation of LDL, while some other studies have observed an opposite effect. To comprehensively clarify the role of HDL in this context, we isolated LDL, HDL2 and HDL3 from sera of 61 free-living individuals (33 women and 28 men).

Results
When the isolated LDL was subjected to Cu2+-induced oxidation, both HDL2 and HDL3 particles increased the rate of appearance and the final concentration of conjugated dienes similarly in both genders. Oxidation rate was positively associated with polyunsaturated fatty acid content of the lipoproteins in that it was positively related to the content of linoleate and negatively related to oleate. More saturated fats thus protected the lipoproteins from damage.

Conclusion
We conclude that in vitro HDL does not protect LDL from oxidation, but is in fact oxidized fastest of all lipoproteins due to its fatty acid composition, which is oxidation promoting.
Human trophoblast function during the implantation process
Authors: Staun-Ram Elsebeth , Shalev Eliezer 
Published: October 2005

AbstractThe implantation process involves complex and synchronized molecular and cellular events between the uterus and the implanting embryo. These events are regulated by paracrine and autocrine factors. Trophoblast invasion and migration through the uterine wall is mediated by molecular and cellular interactions, controlled by the trophoblast and the maternal microenvironment. This review is focused on the molecular constituents of the human trophoblast, their actions and interactions, including interrelations with the uterine endometrium.
The complete chloroplast DNA sequences of the charophycean green algae Staurastrum and Zygnema reveal that the chloroplast genome underwent extensive changes during the evolution of the Zygnematales
Authors: Turmel Monique , Otis Christian , Lemieux Claude 
Published: October 2005

Abstract

Background

The Streptophyta comprise all land plants and six monophyletic groups of charophycean green algae. Phylogenetic analyses of four genes from three cellular compartments support the following branching order for these algal lineages: Mesostigmatales, Chlorokybales, Klebsormidiales, Zygnematales, Coleochaetales and Charales, with the last lineage being sister to land plants. Comparative analyses of the Mesostigma viride (Mesostigmatales) and land plant chloroplast genome sequences revealed that this genome experienced many gene losses, intron insertions and gene rearrangements during the evolution of charophyceans. On the other hand, the chloroplast genome of Chaetosphaeridium globosum (Coleochaetales) is highly similar to its land plant counterparts in terms of gene content, intron composition and gene order, indicating that most of the features characteristic of land plant chloroplast DNA (cpDNA) were acquired from charophycean green algae. To gain further insight into when the highly conservative pattern displayed by land plant cpDNAs originated in the Streptophyta, we have determined the cpDNA sequences of the distantly related zygnematalean algae Staurastrum punctulatum and Zygnema circumcarinatum.

Results

The 157,089 bp Staurastrum and 165,372 bp Zygnema cpDNAs encode 121 and 125 genes, respectively. Although both cpDNAs lack an rRNA-encoding inverted repeat (IR), they are substantially larger than Chaetosphaeridium and land plant cpDNAs. This increased size is explained by the expansion of intergenic spacers and introns. The Staurastrum and Zygnema genomes differ extensively from one another and from their streptophyte counterparts at the level of gene order, with the Staurastrum genome more closely resembling its land plant counterparts than does Zygnema cpDNA. Many intergenic regions in Zygnema cpDNA harbor tandem repeats. The introns in both Staurastrum (8 introns) and Zygnema (13 introns) cpDNAs represent subsets of those found in land plant cpDNAs. They represent 16 distinct insertion sites, only five of which are shared by the two zygnematalean genomes. Three of these insertions sites have not been identified in Chaetosphaeridium cpDNA.

Conclusion

The chloroplast genome experienced substantial changes in overall structure, gene order, and intron content during the evolution of the Zygnematales. Most of the features considered earlier as typical of land plant cpDNAs probably originated before the emergence of the Zygnematales and Coleochaetales.
Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm)
Authors: Echigo Akinobu , Hino Miki , Fukushima Tadamasa , Mizuki Toru , Kamekura Masahiro , Usami Ron 
Published: October 2005

Abstract

Background
Generally, extremophiles have been deemed to survive in the extreme environments to which they had adapted to grow. Recently many extremophiles have been isolated from places where they are not expected to grow. Alkaliphilic microorganisms have been isolated from acidic soil samples with pH 4.0, and thermophiles have been isolated from samples of low temperature. Numerous moderately halophilic microorganisms, defined as those that grow optimally in media containing 0.5–2.5 Molar (3–15%) NaCl, and halotolerant microorganisms that are able to grow in media without added NaCl and in the presence of high NaCl have been isolated from saline environments such as salterns, salt lakes and sea sands. It has tacitly been believed that habitats of halophiles able to grow in media containing more than 20% (3.4 M) are restricted to saline environments, and no reports have been published on the isolation of halophiles from ordinary garden soil samples.

Results
We demonstrated that many halophilic bacteria that are able to grow in the presence of 20% NaCl are inhabiting in non-saline environments such as ordinary garden soils, yards, fields and roadways in an area surrounding Tokyo, Japan. Analyses of partial 16S rRNA gene sequences of 176 isolates suggested that they were halophiles belonging to genera of the family Bacillaceae, Bacillus (11 isolates), Filobacillus (19 isolates), Gracilibacillus (6 isolates), Halobacillus (102 isolates), Lentibacillus (1 isolate), Paraliobacillus (5 isolates) and Virgibacillus (17 isolates). Sequences of 15 isolates showed similarities less than 92%, suggesting that they may represent novel taxa within the family Bacillaceae.

Conclusion
The numbers of total bacteria of inland soil samples were in a range from 1.4 × 107/g to 1.1 × 106/g. One tenth of the total bacteria was occupied by endospore-forming bacteria. Only very few of the endospore-forming bacteria, roughly 1 out of 20,000, are halophilic bacteria. Most of the halophilic bacteria were surviving as endospores in the soil samples, in a range of less than 1 to about 500/g soil. Samples collected from seashore in a city confronting Tokyo Bay gave the total numbers of bacteria and endospores roughly 1000 time smaller than those of inland soil samples. Numbers of halophilic bacteria per gram, however, were almost the same as those of inland soil samples. A possible source of the halophilic endospore originating from Asian dust storms is discussed.
Verbs, Time, and Modality*
Authors: Steedman M. J. 
Published: October 2005

In the first part of this paper it is argued that Vendler's classification of verbs into aspectual categories, called activities, accomplishments, achievements, and states, is better seen as classifying the meanings of sentences, and a recursive scheme for describing the aspectual character of sentences is presented.In the second part, this scheme is applied to the discussion of the epistemic and deontic meanings of the modal verbs must, will, and may. In particular, the relation between the “future” and “nonfuture” senses of epistemic will is examined.
Dialogue‐Games: Metacommunication Structures for Natural Language Interaction*
Authors: Levin James A. , Moore James A. 
Published: October 2005

Our studies of naturally occurring human dialogue have led to the recognition of a class of regularities which characterize impoltant aspects of communication. People appear to interact according to established patterns which span several turns in a dialogue and which recur frequently. These patterns appear to be organized around the goals which the dialogue serves for each participant. Many things which are said later in a dialogue can only be interpreted as pursuit of these goals, established by earlier dialogue.These patterns have been represented b) a set of knowledge structures called Dialogue‐Games, capturing shared, conventional knowledge that people have about communication and how it can be used to achieve goals. A Dialogue‐Game has Parameters, which represent those elements that vary across instances of a particular pattern‐the particular dialogue participants and the content topic. The states of the world which must be in effect for a particular Dialogue‐Game to be employed successfully are represented by Specifications of these Parameters. Finally, the expected sequence of intermediate states that occur during instances of a particular conventional pattern are represented by the Components of the corresponding Dialogue‐Game.Representations for several Dialogue‐Games are presented here, based on our analyses of different kinds of naturally occurring dialogue. A process model is discussed, showing Dialogue‐Game identification, pursuit, and termination as part of the comprehension of dialogue utterances. This Dialogue‐Game model captures some of the important functional aspects of language, especially indirect uses to achieve implicit communication.
Elements of a Plan‐Based Theory of Speech Acts*
Authors: Cohen Philip R. , Perrault C. Raymond 
Published: October 2005

This paper explores the truism hat people think about what hey say. It proposes that, to satisfy heir own goals, people often plan their speech acts to affect their listeners' beliefs, goals, and emotional states. Such language use can be modelled by viewing speech acts as operators in a planning system, thus allowing both physical and speech acts to be integrated into plans.Methodological issues of how speech acts should be defined in a plan‐based theory are illustrated by defining operators for requesting and informing. Plans containing those operators are presented and comparisons are drawn with Searle's formulation. The operators are show to be inadequate since they cannot be composed to form questions (requests to inform) and multiparty requests (requests to request). By refining the operator definitions and by identifying some of the side effects of requesting, compositional adequacy is achieved. The solution leads to a metatheoretical principle for modelling speech acts as planning operators.
Meta‐Planning: Representing and Using Knowledge About Planning in Problem Solving and Natural Language Understanding*
Authors: Wilensky Robert 
Published: October 2005

This paper is concerned with those elements of planning knowledge that are common to both understanding someone else's plan and creating a plan for one's own use. This planning knowledge can be divided into two bodies: Knowledge about the world, and knowledge about the planning process itself. Our interest here is primarily with the latter corpus. The central thesis is that much of the knowledge about the planning process itself can be formulated in terms of higher‐level goals and plans called meta‐goals and meta‐plans. These entities can then be used by the same understanding and planning mechanisms that process ordinary goals and plans. However, the meta‐planning knowledge now enables these mechanisms to handle much more complicated situations in a uniform manner.Systems based on meta‐planning would have a number of advantages over existing problem solving and understanding systems. The same knowledge could be shared by both a planner and understander, and both would be able to handle complex situations elegantly. In addition, in planning, the use of meta‐planning has several advantages over more traditional methods involving constraints or critics. Meta‐planning allows the full power of a problem solver to be applied to situations that are generally amenable only to special purpose processing. In addition, meta‐planning facilitates the representation of some situations that are difficult to express otherwise. We have begun to introduce meta‐planning knowledge into two systems: PAM, a story understanding program, and PANDORA, a problem solving and plannin̈g system.
An analysis of high-cost users at an Australian public health service organization
Authors: Heslop Liza , Athan Dean , Gardner Brendon , Diers Donna , Poh Boon Choo 
Published: October 2005

High-cost users generate extremely high costs when compared with average users in the same diagnostic-related group (DRG). They represent a major financial loss for a health service organization. The research was conducted using an area health service patient database for online analytical processing to produce descriptive statistics and graphs of 'high-cost' and 'non-high-cost users'. Trends and patterns were identified across key variables derived from clinical, financial and operational categories. The main results are: • 20% of costs are spent by 3% of the population; • elective admission is higher in the high-cost group; • tracheostomy has the most number of cases and is the most expensive DRG; • LOS is mostly longer for complex cases however, high costs can be attributed to other factors. In conclusion, these findings are potentially useful to patients, medical staff, management and health service decision-makers. The limitation of this study is the exclusion of profitability.
Can we use routine data to evaluate organizational change? Lessons from the evaluation of business process re-engineering in a UK teaching hospital
Authors: Brennan A , Sampson F , Deverill M 
Published: October 2005

Objective: To review and critically evaluate a multidisciplinary independent evaluation of business process re-engineering within a UK hospital using routine data. Methods: Routine data measures of hospital cost efficiency are used to compare rates of changes at Leicester Royal Infirmary (LRI) with a 'peer group' of 22 teaching hospitals and to ascertain changes attributable to re-engineering. Different adjustment factors are quantified and the robustness of individual measures discussed. In addition to providing context to the quantitative primary research and the qualitative research on change management, service performance and quality indicators not captured in overall efficiency measures are assessed using detailed routine data measures. Results: LRI is one of the most efficient teaching hospitals in England and continued improvement during re-engineering is shown to be at a faster rate than the peer group average, although attribution of changes is difficult. Lack of availability and inconsistency of data for support services meant that many re-engineering initiatives are not quantifiable using routine data measures. Conclusions: When combining different measures, routine data is a valuable tool in evaluating organizational change initiatives. However, use in future evaluations would require consistent benchmarking of routine data.