bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRMT5 inhibition sensitizes glioblastoma tumor models to temozolomide
Authors: Onishi Shumpei , Jayamohan Sridharan , Chowdhury Ashis , Rivas Sarah , Otani Yoshihiro , Murphy Sara A. , Rivera-Caraballo Kimberly A. , Walbridge Stuart , Shah Ashish H. , Sisay Bayu , Maric Dragan , Elkahloun Abdel , Johnson Kory , Heiss John , Lee Tae Jin , Kumbar Sangamesh G. , Brown Desmond A. , Yoo Ji Young , Brenner Andrew , Kaur Balveen , Sareddy Gangadhara R. , Banasavadi-Siddegowda Yeshavanth Kumar 
Published: February 2025

Abstract

Background: Despite multi-model therapy of maximal surgical resection, radiation, chemotherapy, and tumor-treating fields, glioblastoma patients show dismal prognosis. Protein Arginine Methyltransferase 5 (PRMT5) is overexpressed in glioblastoma and its inhibition imparts an anti-tumor effect. Even though Temozolomide (TMZ) is the standard chemotherapeutic agent in the treatment of glioblastoma, tumor cells invariably develop resistance to TMZ. However, the mechanistic role of PRMT5 in glioblastoma therapy resistance is unknown.
Methods: Patient-derived primary glioblastoma neurospheres (GBMNS), treated with PRMT5 inhibitor (LLY-283) or transfected with PRMT5 target-specific siRNA were treated with TMZ and subjected to in vitro functional and mechanistic studies. The intracranial mouse xenograft model was used to test the in vivo antitumor efficacy of combination treatment.
Results: We found that PRMT5 inhibition increased the cytotoxic effect and caspase 3/7 activity of TMZ in GBMNS suggesting that apoptosis is the potential mode of cell death in the combination treatment. PRMT5 inhibition abrogated the TMZ-induced G2/M cell cycle arrest. Unbiased transcriptomic studies indicate that PRMT5 inhibition negatively enriches DNA damage repair genes. Importantly, combination therapy increased DNA double-strand breaks (ɣH2AX foci) and enhanced the DNA damage (comet assay), suggesting that the combination treatment increases the TMZ-induced DNA damage. Specifically, the LLY-283 treatment blocked homologous recombination repair in GBMNS. In vivo, LLY-283 and TMZ combination significantly curbed the tumor growth and prolonged the survival of tumor-bearing mice.
Conclusion: Concomitant treatment of LLY-283 and TMZ has significantly greater antitumor efficacy, suggesting that PRMT5 inhibition and TMZ combination could be a new therapeutic strategy for glioblastoma.
Integrated assessment of yam mosaic virus (YMV) resistance in wild-yam (Dioscorea spp.) interspecific cross progenies using field phenotyping, molecular and biotechnological approaches.
Authors: Oyedoyin Femi Patrick , Kolade Olufisayo , Agre Paterne Angelot , Olawuyi Odunayo Joseph , Kumar Lava , Asfaw Asrat 
Published: February 2025

Abstract

Yams (Dioscorea spp.) are important food crops for millions in tropical and subtropical regions. Yield loss due to Yam Mosaic Virus (YMV genus Potyvirus) has been reported to have a high level of damage, especially on white Guinea yam (D. rotundata). Crop wild relatives often serve as potential sources of alleles that are absent in many cultivated crop species, howevertheir potential for source new disease resistance alleles in yam plants remains underexplored. Therefore, this study evaluated YMV resistance in interspecific hybrids derived from two wild yam specieses, Dioscorea abyssinica as female parent and Dioscorea praehensilis as male parent, for trait introgression. Sixty-five wild yam hybrid genotypes and their parents (maternal-TDabwild and paternal-TDPr 1018) were planted on the field exposed to natural infestation. Field phenotyping and disease severity ratings (1- asymptomatic, 2- resistant, 3- moderately resistant, 4- susceptible and 5- highly susceptible) were combined with molecular analyses using three diagnostic SNP markers, Biotechnology tool ELISA, and RT-PCR to predict, detect and quantify YMV disease resistance and susceptibility patterns. Significant variability in disease resistance was observed, with hybrids exhibiting a wide range of area under disease progress curve (AUDPC) values. Genotypes with the lowest AUDPC values (63) displayed enhanced resistance, while those with higher values (197–231) were more susceptible, reflecting a higher overall disease impact. The ELISA and RT-PCR findings further revealed diverse responses to YMV among the progenies and parents. Two markers, snpDR00160 and snpDR00162, displayed favourable resistant alleles GG and AA, respectively, and susceptible allele TT while marker snpDR00161 failed to amplify across all the progenies. Six progenies (TDabp2101007, TDabp2101009, TDabp2101015, TDabp2101021, TDabp2101026, and TDabp2101060) together with the male parent (D. praehensilis -TDPr 1018) demonstrated putative resistance across phenotypic, molecular, and biotechnological assessments, making them as promising candidates for yam breeding programs aimed at developing YMV-resistant cultivars. Word Count: 299
Effects of MS-222 on the physiological and biochemical response, histology, gut bacteria and flesh quality in largemouth bass (Micropterus salmoides)
Authors: Gao Ping , Chen Rimeng , Lin ShanShan , Yu Haodong , Zhang Xuezhen 
Published: February 2025

Abstract

This study investigated the effects of MS-222 on various biochemical indices, antioxidant status, nonspecific immune response, histomorphology (gill and liver), gut bacteria, and flesh qualities in largemouth bass (Micropterus salmoides). We first evaluated the anaesthetic effects of MS-222 at different concentrations (20, 40, 60, 80, 100 mg/L) on bass (~ 468.34 g) and fish can reach state of deep sedation at 40 mg/L which suitable for liver transportation. Subsequently, the fish were exposed to 40 mg/L MS-222 for 24 h and then transferred to clean water for recovery. Serum, gill, liver, intestine, and back muscle samples were collected at 6, 12, and 24 h post-treated and at the same intervals post-recovery. The results showed that the concentrations of serum cortisol, glucose, LDH, and AST did not significantly change during sedation or recovery periods, although the BUN level significantly increased. The activity of antioxidase was not significantly affected, but the malondialdehyde (MDA) concentration decreased during sedation period. MS-222 induced atrophy and hypertrophy in gill filaments, decreased the nucleolus number, and increased vacuolation in the liver; these changes were not fully reversed by 24 h post-recovery. MS-222 treatment disrupted gut bacteria, reducing the abundance of beneficial bacteria such as Cetobacterium. An increase in flavour substances, such as sweet and umami amino acids, betaine, and nucleotides, enhanced the flavour of the muscle during sedation and recovery periods. Additionally, MS-222 decreased the proportion of monounsaturated fatty acids (MUFAs) and increased the proportion of polyunsaturated fatty acids (PUFAs). Analysis of electronic nose data indicated that MS-222 treatment distinguished the flavor of fish at sedation period, but diminished during the recovery period. This study demonstrates the influences of MS-222 at 40 mg/L on the physiology, biochemistry and flesh qualities of largemouth bass, which provides a reference for the application of MS-222 in live transportation of largemouth bass.
Aptamer-regulated colorimetric and electrochemical dual-mode sensor for the detection of uranyl ions utilizing AuNCs@COF composite
Authors: Chen Zhijun , Liu Jinquan , Wang Wenyu , Qin Guoqing , Liu Siru , Zhang Weilin , Peng Changmin , Tan Yan , Dai Zhongran , Zhen Deshuai , Li Le 
Published: February 2025

Abstract

In this context, an innovative dual-mode colorimetric and electrochemical sensor was developed for the detection of uranyl ions (UO22+), utilizing a covalent organic framework@gold nanoclusters (AuNCs@COF) composite. The synthesis of AuNCs@COF was simple, and the incorporation of AuNCs imparted the composite with exceptional peroxidase-like catalytic activity and enhanced electrochemical properties. By regulating the adsorption and desorption of aptamers on the AuNCs@COF surface, both peroxidase-like activity and conductivity were modulated, enabling the detection of UO22+ utilizing colorimetric and electrochemical dual signals. Under optimal conditions, the sensor revealed a broad linear detection range and a low detection limit, with ranges of 1.36×10-10 - 1.36×10-5 mol/L for colorimetric detection and 5.0×10-10 - 2.5×10-5 mol/L for electrochemical detection, achieving detection limits of 107 pmol/L and 347 pmol/L, respectively. Unlike other single-mode sensor for UO22+ detection, this dual-mode sensor demonstrated superior sensitivity, specificity, and repeatability. Furthermore, the results of spiked recovery experiments in real water samples highlight the promising potential of this dual-mode sensor for environmental water monitoring applications.
Dual-Band Absorptive Bandpass Filters with All- Frequency Reflectionless Performance and Enhanced Upper-Band Suppression
Authors: Wu Gangxiong , Jin Yang , Zhang Wei , Jiang Ruirui , Shi Jin 
Published: February 2025

Abstract

This paper presents a novel dual-band bandpass filter (BPF) with two-port all-frequency reflectionless characteristics and enhanced upper-band suppression. The design integrates a square ring resonator (SRR) and advanced coupled lines (CLs), incorporating complementary absorptive branches to achieve broadband reflectionless performance. To mitigate upper-band harmonics, radial-stub-loaded resonators (RSLSs) with high-impedance transmission lines (HITLs) are introduced, extending suppression up to 10f0 without significantly increasing the circuit footprint. A comprehensive theoretical analysis, including parametric studies and transmission zero (TZ) derivation, is conducted to optimize performance. Two prototypes are designed, fabricated, and tested: Prototype I achieves dual-band operation with all-frequency absorption, while Prototype II further enhances harmonic suppression. Experimental results closely align with simulations, with Prototype I exhibiting 3-dB fractional bandwidths (FBWs) of 19.5% and 9.7% at 1.38 GHz and 2.63 GHz, respectively, with insertion losses (ILs) of 0.95 dB and 1.47 dB. Prototype II achieves superior harmonic suppression, with attenuation levels of 22.8 dB over 2.9-20 GHz and 28.5 dB within 3.0–12 GHz. These designs offer high selectivity, inter-band isolation exceeding 45 dB, and full-spectrum absorption, providing an efficient solution for modern RF and wireless communication systems.
Characterization of nitrate pollution in the Mio-Plio-Quaternary groundwater body of the Boughezel region, Eastern Algeria
Authors: Ziani Dalila , Maaliou Aziz , Djekoune Leila , Moussouni Abderzak 
Published: February 2025

ABSTRACT

This study examines the spatio-temporal evolution of nitrate levels in the groundwater of the Boughezel region in eastern Algeria. The study area is located in Mio-Plio-Quaternary alluvium and is characterized by an aquifer influenced by intensive agricultural activities. Twenty-one water samples were collected during two campaigns, corresponding to low and high-water periods, allowing for seasonal variations in water quality to be captured. Nitrate concentrations were measured, and results show that nitrate levels exceed World Health Organization standards in approximately 50% of the analyzed water points, reaching up to 111 mg/L during the high-water period. An in-depth statistical analysis identified a significant correlation between nitrate levels and agricultural practices, highlighting the significant impact of anthropogenic activities on groundwater quality. This study underscores the need for management strategies, such as regulating nitrogen fertilizer use and reducing the discharge of untreated wastewater into the natural environment, to mitigate nitrate contamination.
Степень порицания коррупции у россиян с разным уровнем отношения к социальному доминированию
Authors: Максименко Александр А. , Крылова Дина В. , Дейнека Ольга С. 
Published: February 2025

Introduction. Status relationships and status perceptions are the main driving forces for behavior, self-perception, and the individual picture of the world. This empirical study aims to investigate the relationship between moral outrage over corruption among Russians and their attitudes toward social dominance. In addition, the social dominance orientation (SDO) scale was approved and validated. Methods. A total of 509 subjects participated in an online survey (mean age 41.34 years, SD = 10.67; 57.6 % males, 42.4 % females). The study used the Social Dominance Orientation scale, the 5-point scale to measure awareness of corruption (General Social Survey), the 4-point scale to measure outrage over corruption, and the Short Dark Triad questionnaire. The data were processed with the SPSS 19.0 software. Results. The results of the study showed a positive relationship between the degree of moral outrage over corruption and the approval of the social hierarchy among Russians. The exploratory factor analysis of the data from the Social Dominance Orientation scale made it possible to distinguish the following three factors: (a) “the idea of social equality (anti-egalitarianism)”, (b) “the idea of social dominance (egalitarianism)”, and (c) “the idea of rivalry (competition)”. The correlation analysis showed significant correlations between personality traits of respondents (the parameters of the dark triad) and their attitudes toward social dominance. Discussion. The findings of this study are confirmed by the results of previous studies on the impact of social dominance orientation on the low level of awareness of corruption, which in turn contributes to the desire to maintain the social hierarchy and strengthens corrupt intention. Furthermore, according to the results of foreign studies, psychological predictors for corrupt intention are competitive world beliefs and dangerous world beliefs. This requires additional verification and is the objective of our future research.
Caring for the Community: The Effects of Client Civic Benefit on Auditors’ Stakeholder Prioritizations and Audit Judgments
Authors: Knutson Macy J. , Stefaniak Chad M. , Brown Timothy J. 
Published: February 2025

SYNOPSIS
We investigate whether a for-profit firm’s civic benefit (i.e., the direct societal benefit provided through the organization’s primary operations) affects how auditors prioritize nonfinancial stakeholders, and whether that prioritization influences auditors’ judgments. Our experiment shows auditors (1) prioritize nonfinancial stakeholders (the community) above financial stakeholders (a bank), and (2) are less likely to convey information about significant client risks when auditing clients with more civic benefit. Our results provide initial evidence that auditors’ consideration of relevant stakeholders not only extends to nonfinancial stakeholders, but also is malleable based on client-specific factors. Although potentially noble, the prioritization of nonfinancial stakeholders runs counter to extant professional guidance. Accordingly, our results could be important to regulators who may have to address how auditors should incorporate nonfinancial stakeholders into their judgment framework. Moreover, our results inform auditors who are auditing higher civic benefit organizations more frequently given the increasing corporate focus on prosocial activities.
Data availability: Data are available upon request.
EDGAR Implementation, Unionization, and Strategic Disclosure
Authors: Aobdia Daniel , Cheng Lin , Tan Qin , Wu Xuan 
Published: February 2025

ABSTRACT
This study focuses on the effect of disclosure processing frictions in labor markets. We go back in time 30 years ago and examine whether firms facing strong organized labor strategically responded to the implementation of the Electronic Data Gathering, Analysis, and Retrieval (EDGAR) system, which substantially reduced labor unions’ information processing costs. Consistent with firms having incentives to maintain an information advantage over unions for bargaining purposes, we find that they reduce financial statement disaggregation, the likelihood and frequency of management forecasts, and the proportion of good news forecasts. Our study is the first to investigate the implications of information processing costs for labor markets and suggests that an SEC mandate intended to reduce disclosure processing costs for investors caused unintended strategic responses by firms facing proprietary cost of disclosures in other markets.
Data Availability: Data are available from sources identified in the text.
JEL Classifications: M40; M41; J51; J52.
The Burden of Diabetes Mortality by County, Race, and Ethnicity in the U.S., 2000–2019
Authors: Nassereldine Hasan , Li Zhuochen , Compton Kelly , Kendrick Parkes , Kahn Ethan , Kelly Yekaterina O. , Baumann Mathew M. , Schmidt Chris A. , Sylte Dillon O. , Ong Kanyin Liane , La Motte-Kerr Wichada , Daoud Farah , McLaughlin Susan A. , Hay Simon I. , Rodriquez Erik J. , Nápoles Anna M. , Mensah George A. , Pérez-Stable Eliseo J. , Mokdad Ali H. , Dwyer-Lindgren Laura 
Published: February 2025

OBJECTIVE
Diabetes is a leading cause of death in the U.S. Previous studies have found substantial racial, ethnic, and geographical disparities in diabetes mortality; however, research considering racial, ethnic, and geographical disparities simultaneously has been limited. To fill this gap, we estimated trends in diabetes mortality rates from 2000 to 2019 at the county level for five racial and ethnic populations.

RESEARCH DESIGN AND METHODS
We applied small-area estimation methods to death registration data from the U.S. National Vital Statistics System and population data from the U.S. National Center for Health Statistics and corrected for misclassification of race and ethnicity on death certificates.

RESULTS
Age-standardized diabetes mortality rates decreased in the U.S. from 28.1 deaths per 100,000 (95% uncertainty interval 27.9–28.2) in 2000 to 19.1 deaths per 100,000 (19.0–19.2) in 2019. In 2019, national-level rates were highest for the American Indian or Alaska Native (AIAN) population (35.6 [32.1–39.4]), followed by the Black (31.9 [31.5–32.3]), Latino (19.7 [19.3–20.2]), White (17.6 [17.5–17.8]), and Asian (12.6 [12.1–13.1]) populations. There was substantial heterogeneity in diabetes mortality rates across counties within each racial and ethnic population, with the AIAN population experiencing the greatest heterogeneity in 2019 (interquartile range 18.7–50.3 [median 31.9]). For each racial and ethnic population, mortality rates declined in most counties from 2000 to 2019.

CONCLUSIONS
Since 2000, progress has been made in reducing diabetes mortality rates. Nonetheless, diabetes mortality remains too high for many Americans. Interventions focusing on communities at highest risk are vital to resolving persistent health inequities.
Sir Francis Galton's Philosophy of Measurement Science and Its Relevance for the Development of the Psychometric Paradigm
Authors: Nugraha Dwi Yan , Kuswanjono Arqom 
Published: February 2025

This article discusses the origins of the psychometric paradigm and its reliance on the notion that mental attributes can be measured. Galton's assumption that mental attributes can be discerned through definite actions in response to definite situations is misguided. This article highlights the ternary nature of causality, involving causes, effects, and specific causal fields. The same cause may not produce the same effect in different causal fields, and different causes may produce the same effect in different causal fields. The relationships between observable measures and aspects of character are unclear, and indirect indices of mental attributes are inherently ambiguous. This article focuses on Francis Galton's role in establishing these beliefs and argues that his reasoning was flawed and influenced by Pythagoras' philosophy of science and his desire to present eugenics as science. This paper aims to describe Sir Francis Galton's theory regarding the science of measurement and its relevance to psychometrics. Using the literature review method on various sources (scientific articles and textbooks) related to the topic of discussion. Overall, this article challenges the assumption that mental attributes are inherently quantitative and argues for a more nuanced understanding of measurement in psychometrics.
Membangun Kesadaran Ekologis di Era Digital: Eksplorasi Filsafat Nilai Max Scheler dalam Konten Pandawara Group
Authors: Dony Ahmad Rama 
Published: February 2025

Permasalahan serius terkait sampah telah menjadi katalisator utama dalam krisis lingkungan hidup global saat ini. Tantangan ini mendorong berbagai kalangan untuk merumuskan solusi konkret menghadapi dampak negatif dari manajemen sampah yang belum optimal. Dalam era digital, pertautan antara filsafat nilai dan konten digital menjadi semakin penting, konten digital, khususnya yang tersebar melalui media sosial, mengandung nilai-nilai yang memerlukan eksplorasi mendalam guna memahami implikasi etisnya terhadap transformasi pola pikir dan perilaku masyarakat secara luas. Penelitian ini bertujuan untuk menemukan serta mendeskripsikan nilai-nilai yang ada dalam aksi dan publikasi konten penanganan sampah oleh Pandawara Group dalam perspektif filsafat nilai Max Scheler. Metode yang digunakan adalah penelitian kualitatif dengan pendekatan analisis deskriptif. Hasil penelitian ini menggambarkan sejumlah nilai-nilai yang tercermin dalam aksi dan publikasi konten Pandawara Group terkait dengan penanganan sampah. Pertama, munculnya nilai kebahagiaan, yang ditemukan dalam dampak positif yang dirasakan oleh pengguna media secara umum ketika berinteraksi dengan konten Pandawara Group. Kedua, munculnya nilai vital, yang tercermin dalam upaya penyadaran kembali terhadap kesejahteraan umum dan kesehatan. Ketiga, terdapat penggugah kepedulian spiritual terhadap lingkungan sekitar bagi pengguna media sosial dengan menunjukkan keterkaitan antara dimensi spiritual dengan pelestarian lingkungan. Terakhir, pada tahap tertinggi, munculnya nilai-nilai kesucian, yang tercermin dalam penghargaan terhadap hak-hak alam yang harus di jaga. Pandawara Group telah menjelma sebagai suatu entitas yang bukan hanya berperan sebagai agen perubahan, melainkan juga memberikan dampak positif yang signifikan dalam menciptakan nilai-nilai kesadaran, kebijaksanaan, dan kesejahteraan di kalangan masyarakat dalam upaya menjaga kelestarian lingkungan.
Argumentación constitucional en un caso de conflicto entre principios
Authors: Rocca Mundin María Elena 
Published: February 2025

El presente trabajo procura describir y analizar el razonamiento y los argumentos empleados en un caso en el que se presentaban en conflicto principios e intereses constitucionales: el de libertad, interés general e igualdad y en el que recayó sentencia dictada por la Suprema Corte de Justicia Uruguaya luego de la tramitación de un proceso de control de constitucionalidad de la ley.