bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-Associated Proteins in Saccharomyces cerevisiae
Authors: Auld Kathryn L , Hitchcock Amy L , Doherty Hugh K , Frietze Seth , Huang Linda S , Silver Pamela A 
Published: July 2006

Abstract
The regulation of cellular membrane dynamics is crucial for maintaining proper cell growth and division. The Cdc48-Npl4-Ufd1 complex is required for several regulated membrane-associated processes as part of the ubiquitin–proteasome system, including ER-associated degradation and the control of lipid composition in yeast. In this study we report the results of a genetic screen in Saccharomyces cerevisiae for extragenic suppressors of a temperature-sensitive npl4 allele and the subsequent analysis of one suppressor, GET3/ARR4. The GET3 gene encodes an ATPase with homology to the regulatory component of the bacterial arsenic pump. Mutants of GET3 rescue several phenotypes of the npl4 mutant and transcription of GET3 is coregulated with the proteasome, illustrating a functional relationship between GET3 and NPL4 in the ubiquitin–proteasome system. We have further found that Get3 biochemically interacts with the trans-membrane domain proteins Get1/Mdm39 and Get2/Rmd7 and that Δget3 is able to suppress phenotypes of get1 and get2 mutants, including sporulation defects. In combination, our characterization of GET3 genetic and biochemical interactions with NPL4, GET1, and GET2 implicates Get3 in multiple membrane-dependent pathways.
Drosophila Reptin and Other TIP60 Complex Components Promote Generation of Silent Chromatin
Authors: Qi Dai , Jin Haining , Lilja Tobias , Mannervik Mattias 
Published: July 2006

AbstractHistone acetyltransferase (HAT) complexes have been linked to activation of transcription. Reptin is a subunit of different chromatin-remodeling complexes, including the TIP60 HAT complex. In Drosophila, Reptin also copurifies with the Polycomb group (PcG) complex PRC1, which maintains genes in a transcriptionally silent state. We demonstrate genetic interactions between reptin mutant flies and PcG mutants, resulting in misexpression of the homeotic gene Scr. Genetic interactions are not restricted to PRC1 components, but are also observed with another PcG gene. In reptin homozygous mutant cells, a Polycomb response-element-linked reporter gene is derepressed, whereas endogenous homeotic gene expression is not. Furthermore, reptin mutants suppress position-effect variegation (PEV), a phenomenon resulting from spreading of heterochromatin. These features are shared with three other components of TIP60 complexes, namely Enhancer of Polycomb, Domino, and dMRG15. We conclude that Drosophila Reptin participates in epigenetic processes leading to a repressive chromatin state as part of the fly TIP60 HAT complex rather than through the PRC1 complex. This shows that the TIP60 complex can promote the generation of silent chromatin.
A First-Generation Metric Linkage Disequilibrium Map of Bovine Chromosome 6
Authors: Khatkar Mehar S , Collins Andrew , Cavanagh Julie A L , Hawken Rachel J , Hobbs Matthew , Zenger Kyall R , Barris Wes , McClintock Alexander E , Thomson Peter C , Nicholas Frank W , Raadsma Herman W 
Published: July 2006

Abstract
We constructed a metric linkage disequilibrium (LD) map of bovine chromosome 6 (BTA6) on the basis of data from 220 SNPs genotyped on 433 Australian dairy bulls. This metric LD map has distances in LD units (LDUs) that are analogous to centimorgans in linkage maps. The LD map of BTA6 has a total length of 8.9 LDUs. Within the LD map, regions of high LD (represented as blocks) and regions of low LD (steps) are observed, when plotted against the integrated map in kilobases. At the most stringent block definition, namely a set of loci with zero LDU increase over the span of these markers, BTA6 comprises 40 blocks, accounting for 41% of the chromosome. At a slightly lower stringency of block definition (a set of loci covering a maximum of 0.2 LDUs on the LD map), up to 81% of BTA6 is spanned by 46 blocks and with 13 steps that are likely to reflect recombination hot spots. The mean swept radius (the distance over which LD is likely to be useful for mapping) is 13.3 Mb, confirming extensive LD in Holstein–Friesian dairy cattle, which makes such populations ideal for whole-genome association studies.
Molecular Characterization of teflon, a Gene Required for Meiotic Autosome Segregation in Male Drosophila melanogaster
Authors: Arya Gunjan H , Lodico Matthew J P , Ahmad Omar I , Amin Rohul , Tomkiel John E 
Published: July 2006

Abstract
Drosophila melanogaster males lack recombination and have evolved a mechanism of meiotic chromosome segregation that is independent of both the chiasmatic and achiasmatic segregation systems of females. The teflon (tef) gene is specifically required in males for proper segregation of autosomes and provides a genetic tool for understanding recombination-independent mechanisms of pairing and segregation as well as differences in sex chromosome vs. autosome segregation. Here we report on the cloning of the tef gene and the molecular characterization of tef mutations. Rescue experiments using a GAL4-driven pUAS transgene demonstrate that tef corresponds to predicted Berkeley Drosophila Genome Project (BDGP) gene CG8961 and that tef expression is required in the male germ line prior to spermatocyte stage S4. Consistent with this early prophase requirement, expression of tef was found to be independent of regulators of meiotic M phase initiation or progression. The predicted Tef protein contains three C2H2 zinc-finger motifs, one at the amino terminus and two in tandem at the carboxyl terminus. In addition to the zinc-finger motifs, a 44- to 45-bp repeat is conserved in three related Drosophila species. On the basis of these findings, we propose a role for Tef as a bridging molecule that holds autosome bivalents together via heterochromatic connections.
Melanotic Mutants in Drosophila: Pathways and Phenotypes
Authors: Minakhina Svetlana , Steward Ruth 
Published: July 2006

Abstract
Mutations in &gt;30 genes that regulate different pathways and developmental processes are reported to cause a melanotic phenotype in larvae. The observed melanotic masses were generally linked to the hemocyte-mediated immune response. To investigate whether all black masses are associated with the cellular immune response, we characterized melanotic masses from mutants in 14 genes. We found that the melanotic masses can be subdivided into melanotic nodules engaging the hemocyte-mediated encapsulation and into melanizations that are not encapsulated by hemocytes. With rare exception, the encapsulation is carried out by lamellocytes. Encapsulated nodules are found in the hemocoel or in association with the lymph gland, while melanizations are located in the gut, salivary gland, and tracheae. In cactus mutants we found an additional kind of melanized mass containing various tissues. The development of these tissue agglomerates is dependent on the function of the dorsal gene. Our results show that the phenotype of each mutant not only reflects its connection to a particular genetic pathway but also points to the tissue-specific role of the individual gene.
Consumers' expectations of food and alcohol pairing
Authors: Pettigrew Simone , Charters Steve 
Published: July 2006

PurposeFood and alcohol are symbolically and physically linked in many cultures. This article seeks to explore Australians' perceptions of the relationships between food and two of the more popular forms of alcohol – wine and beer.Design/methodology/approachThe findings from two parallel alcohol studies are reported. One study examined the role of wine consumption in Australian culture (n=105), while the other study performed the same function in terms of beer consumption (n=115). Interviews and focus groups were used in both studies to collect data from consumers and industry representatives to identify expectations of appropriate food and alcohol pairing.FindingsWine was found to be strongly associated with food along three dimensions: complementarity, social meaning, and lubrication. This association occurs in the context that it is generally deemed inappropriate to consume wine without food. By comparison, a much weaker association appears to exist between food and beer. Interviewees reported regularly consuming beer in both eating and non‐eating contexts, with the nature of the occasion influencing beer consumption rather than any food that may or may not be present.Originality/valueConsumers' expectations of the appropriate pairing of food with beer and wine are discussed in terms of their implications for those wishing to align particular food and alcohol products in the marketplace. A theoretical contribution is provided through a discussion of the ways in which different alcoholic beverages are assigned different symbolic meanings to allow them to facilitate divergent food consumption experiences.
Training customers: an organizational experience
Authors: Llopis Juan , Gasco Jose , Gonzalez Reyes 
Published: July 2006

PurposeTo analyze, through a case study, the essential premises on the basis of which a training plan for the customers of any given firm can be designed, implemented and evaluated.Design/methodology/approachThis paper starts with a review of the literature on customer service training, which is followed by a discussion on how a training plan for customers could be elaborated. After that, the case method is applied (mainly through semi‐structured interviews) to a firm that approaches this type of training in an innovative way.FindingsConcludes that a training plan for the firm's internal staff cannot be extrapolated to customers. Describes how training plans must be modified so as to adapt them to customers.Research limitations/implicationsIt will be nesessary to identify other cases and check to what extent the methodology resembles the one we have applied and how successful it is, so that a standard action protocol can be established.Practical implicationsPresents a real case of success at customer training in an industrial firm and provides a model, not only for future research, but also for the direct implementation of these techniques by other firms belonging to any industrial sector.Originality/valueThe accurate description of a training plan for customers made by an industrial firm and its treatment based on the study of a real case. The establishment of action guidelines that will pave the way for further academic research in this field as well as its clear business applicability.
Training an age‐diverse workforce
Authors: Newton Becci 
Published: July 2006

PurposeAims to address the myths and age‐related barriers that currently deter employers from training workers of different ages.Design/methodology/approachThis study is the culmination of four strands of research activity: qualitative interviews with a range of national experts to inform the context for the research and the key issues to be explored. The interviews also helped to identify the training channels available to employers and their workers. Review of the current policy and academic literatures. Secondary data analysis of the Labour Force Survey, 2004 and National Adult Learning Survey, 2002. Also includes LSC data from the Employer Training Pilots and Modern Apprenticeships. Case studies of five organisations which demonstrate good practice in training a mixed‐age workforce. Throughout the study, references to “older workers” mean people aged over 50.FindingsThere is a clear association between age and the amount of training offered to and received by workers. Employees aged over 55 were less likely than other workers to participate in training, or to have been offered it. Older employees were also less likely than younger or mid‐life workers to take up any opportunities for training that were made available. Furthermore, older workers were more likely only to have received on‐the‐job training.Originality/valueProvides the evidence and practical guidance on the variety of training support channels and on age‐diverse training practices.
Enhancing professional development by writing for publication in library and information science
Authors: Joint Nicholas 
Published: July 2006

PurposeTo argue that enhanced professional development and writing for publication are related activities and are mutually beneficial.Design/methodology/approachAn opinion piece.FindingsThat by aiming for a more rigorous form of professional writing, greater insight into one's professional practice is possible.Research limitations/implicationsThe examples of research scenarios sketched out in this editorial are pure suggestions which have not been carried out as real experiments. However, this would be possible and even desirable in order to prove the hypothesis outlined in the paper.Practical implicationsIf these conclusions are valid, then writing for publication has a clear practical benefit for practitioners.Originality/valueThe simple examples of the workplace experiment and case study that this paper briefly sketches out could help practitioners improve the type of “practitioner research” that they undertake.
Job rotation in Nigerian university libraries
Authors: Adomi Esharenana E. 
Published: July 2006

PurposeTo understand the impact of job rotation on Nigerian university libraries and in libraries in general. Design/methodology/approach – L A survey of opinion in Nigerian university libraries. Professional librarians from seven university libraries were used as subjects for the study. Findings That most of the respondents have spent an appreciable period of time working in the libraries; a majority of the respondents have been rotated; most of the respondents see job rotation as opportunity to learn new skills followed by those who see it as opportunity to be acquainted with other operations/services of the library; most of the respondents adjust to the new post by studying handover notes/files of new departments but a majority of them are of the view that job rotation is not regular enough in their libraries.Research limitations/implicationsThe focus of the paper is practical rather than research‐oriented.Practical implicationsSuggestions are made on how job rotation could be improved upon in libraries.Originality/valueThere are few empirical studies of job rotation in the literature – this paper increases understanding of a little studied topic.
Institutional repositories, self‐archiving and the role of the library
Authors: Joint Nicholas 
Published: July 2006

PurposeTo raise some unresolved questions about the practical implementation and management of institutional repositories – in particular, the level of resource needed to support the process of self‐deposit into institutional repositories.Design/methodology/approachAn opinion piece based on practitioner insights into their cataloguing practice and into digital preservation issues.FindingsThat metadata creation and the formulation of digital preservation policies for institutional repositories require significant resource, if they are to be carried out well. This level of resourcing may have been underestimated hitherto in the process of proselytising for institutional repositories.Research limitations/implicationsThis practice‐based supposition could be tested more rigorously by proper research investigation.Practical implicationsSuggests that libraries and librarians are well placed to give input to the metadata and digital preservation activities inherent in building institutional repositories. They should be resourced to give more attention to such tasks.Originality/valueThe paper tries to make a strong case for librarian‐mediated deposit rather than pure self‐archiving as the future of building institutional repositories.
Digital preservation in the tertiary education sector: management implications
Authors: Robertson R. John 
Published: July 2006

PurposeThe purpose of this paper is to assess the future of long‐term curation and preservation of digital assets with particular reference to Further Education (FE) in the UK.Design/methodology/approachReviews current requirements of digital preservation and the efforts underway to support them. Drawing on other recent work and the author’s experience in a recent development project it subsequently comments on these efforts in the context of FE.FindingsArgues that the long‐term curation and preservation of digital assets produced by further education colleges should not be the responsibility of those colleges.Research limitations/implicationsWritten with direct reference to the UK; how the suggested solution would be applied elsewhere remains undeveloped.Practical implicationsNational memory institutions should strive to establish collaborative curatorial practices with the FE sector.Originality/valueSuggests that the preservation of digital assets in the FE sector requires a different approach than the HE sector.
Intransivities of managerial decisions: a grounded theory case
Authors: Douglas David 
Published: July 2006

PurposeThe purpose of this paper is to apply grounded theory methodology to report on an empirical case which develops emergent theories on the human complexities of managerial decision‐making and the synonymous task of managing.Design/methodology/approachA structured research approach was applied in gathering and analysing data from all actors within a small private sector enterprise. The key objective was to discover what the owner‐manager and all employees perceived as important issues with regard to the managing of the business during a period of post‐takeover. In‐depth on‐site and off‐site interviews were carried out over an extended period.FindingsEmergent theory exposes actors' disputed perceptions of how the business had been managed and ought to be managed, and, the judgments and decisions that had been made and consequently should be made. Revealed is a complex cognitive and behavioural web of human interactions and deep‐seated management‐employee discord that whilst threatening the actual survival of the business appears not to impede questionable practices, both by management and staff. Through the application of grounded theory methodology emergent constructs are discussed against existing knowledge that exposes new insights into management decision theory and the managing of an enterprise.Research limitations/implicationsThe process of theory generation whilst grounded in a substantive inquiry has the capacity to generate further research and tentative explanations at higher levels of understanding. From the research reported, questions beyond the substantive case can develop a broader theoretical and practical agenda – for example, issues of other actors' involvement in management decision making and the intrinsic part psychological factors play in the structuring of decisions.Practical implicationsBased on the finding from an empirical study the paper reveals significant practical managerial issues in the day‐to‐day and strategic managing of an enterprise. From a researcher's perspective, the paper critically demonstrates the functionality of grounded theory in management inquiry.Originality/valueThis paper advances the theoretical and practical necessity for the enlargement of the stock of qualitatively bounded research that focuses on grounded theory applications, management practice and decision theory.
Library subject portals
Authors: Ubogu Felix N. , Kekana Arthur , Roberts Charl 
Published: July 2006

PurposeTo examine and discuss the features of library portals and suggest further development of these research tools.Design/methodology/approachThe two categories of library portals (horizontal or public, and vertical) were examined to establish the characteristics of some of the portal software available in the marketplace. The study involved carrying out a literature search and visiting demonstration sites. Some library sites that have implemented this software were subsequently examined in order to establish features of their sites, and to see how these sites have handled the information literacy/research process.FindingsSuggests that a well‐structured subject portal should be based on the principles of the flow of scientific information, and an information literacy component should be embedded in the portal. Such a portal could assist learners to become familiar with the breadth and depth of information resources relevant to their discipline. It should also assist librarians and learning advisers to become familiar with the information literacy requirements of specific subjects and courses. In this way, libraries should achieve their objective of making the learner information‐literate with minimal effort, and thus contribute positively to student learning outcomes.Practical implicationsThe paper is a useful source of information for librarians considering investing in one of the commercial software tools and for those embarking on the development of subject portals. A pilot version of the subject portal implemented at the University of the Witswatersrand is shown.Originality/valueThe novelty in this work is the intuitive subject portal that is put forward; the elements and content of the portals are suggested.
Dislike of vegetables and type 2 diabetes
Authors: Sakuta Hidenari , Suzuki Takashi , Yasuda Hiroko , Ito Teizo 
Published: July 2006

PurposeThe study aims to determine whether dislike of vegetables is associated with the presence of metabolic disorders.Design/methodology/approachCross‐sectional analysis of the data of middle‐aged (range 51–59 years) male personnel of the Self‐Defense Forces.FindingsOf subjects studied, 76.4 per cent answered they liked vegetables, 19.2 per cent were intermediate between liking and dislike and 4.4 per cent disliked vegetables. The odds ratio (95 per cent CI) of vegetable dislike to like was 2.22 (1.08–4.57) for the presence of diabetes, 2.46 (1.23–4.94) for hyper‐triglyceridemia and 2.54 (1.33–4.86) for high γ‐glutamyl transferase in a logistic regression analysis adjusted for age and lifestyle factors. Vegetable dislike did not correlate with hypertension, hypercholesterol‐emia or obesity. Vegetable consumption did not correlate with diabetes.Research limitations/implicationsThe results do not show a cause–result relationship. Observed findings may not be applied to age‐matched general population, or to older, younger, female or other ethnic persons.Practical implicationsVegetable dislike may be regarded as a simple marker of metabolic status including type 2 diabetes.Originality/valueThe authors show the cross‐sectional association between dislike of vegetables and type 2 diabetes.