bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elucidating the Structure of the Eu‐EDTA Complex in Solution at Various Protonation States
Authors: Licup Gerra L. , Summers Thomas J. , Sobrinho Josiane A. , de Bettencourt‐Dias Ana , Cantu David C. 
Published: March 2024

AbstractEthylenediaminetetraacetic acid (EDTA), which has two amine and four carboxylate protonation sites, forms stable complexes with lanthanide ions. This work analyzes the coordination structure, in atomic resolution, of the Eu3+ ion complexed with EDTA in all its protonation states in aqueous solution. Eu‐EDTA complexes were modeled using classical molecular dynamics (MD) simulations using force field parameters optimized with ab initio molecular dynamics (AIMD) simulations. Structures from the MD simulations were used to predict extended X‐ray absorption fine structure (EXAFS) spectra and compared with EXAFS measurements of the Eu3+ aqua ion and Eu‐EDTA complexes at pH 3 and 11. This work details how Eu‐EDTA complex coordination structures change with increasing protonation of the EDTA ligand in the complex, from the tightly bound unprotonated complex to the unbinding of the fully protonated EDTA ligand from the Eu3+ ion as both become solvated by water. Agreement between predicted and measured EXAFS spectra supports the findings from simulation.
The usefulness of NDDI‐E and QOLIE‐10 scales for the screening of major depressive disorders in patients with epilepsy in video‐EEG units
Authors: Mayo Rodríguez Pablo , Parejo‐Carbonell Beatriz , Sanz Graciani Isabel , Romeral Jiménez María , De la Cruz Moreno María Cristina , Lastras Clara , Sánchez‐del‐Hoyo Rafael , García Morales Irene 
Published: March 2024

AbstractObjectiveMood disorders in patients with epilepsy are common, with depression being the most prevalent. However, this comorbidity is often underdiagnosed. The systematic use of scales such as NDDI‐E and QOLIE‐10 in prolonged video‐EEG monitoring units could be a useful tool for the detection of this comorbidity.MethodsDescriptive cross‐sectional study of a series of patients with epilepsy evaluated in a prolonged video‐EEG monitoring unit.ResultsThree hundred forty‐nine patients were included. The mean age was 49.1 years, and 49.3% were female. 66.2% had focal epilepsy. 20.4% had pharmacoresistant epilepsy. 38.7% of patients had NDDI‐E > 13. 43% of patients with focal epilepsy had NDDI‐E > 13 versus 21.8% of patients with idiopathic generalized epilepsy (p = .015). Patients with focal temporal epilepsy had the highest rate of NDDI‐E > 13 (48.5%). Significant association was found between patient‐perceived mood and NDDI‐E score (p < .001). However, in the group of patients with NDDI‐E > 13, 37.6% had reported feeling “very good” or “good” in mood. Likewise, in the group that had reported feeling “very good” or “good” 21.6% had NDDI‐E > 13. In 50.5% of patients with NDDI‐E > 13 some kind of therapeutic intervention aimed at this comorbidity was performed. Perceived quality of life as measured by the QOLIE‐10 scale was lower in patients with NDDI‐E > 13 (p < .001).SignificanceThe use of scales such as the NDDI‐E and QOLIE‐10 at the time of admission in video‐EEG monitoring units allows screening for major depressive disorders in patients with epilepsy, which subsequently needs to be confirmed by formal assessment by a psychiatrist. Their systematic use in these units prevents some patients from going undiagnosed. Detection of these disorders allows targeted therapeutic intervention.
EWA‐DA‐based resource allocation method for 5G networks
Authors: Teja Panduranga Ravi , Mishra Pavan Kumar , Gupta Dharmendra Lal 
Published: March 2024

AbstractIn 5G networks, most D2D work focuses on optimizing system throughput without addressing economic constraints. This article encourages users to exchange resources and apply honest bidding tactics to attain high auction efficiency. This study describes an experience‐weighted attraction double auction (EWA‐DA) technique for allocating resources in 5G D2D networks. The suggested method classifies mobile users into seller cellular users (SCU) and buyer cellular users' (BCU). The SCU transmits the beacon packet, and the nearby cellular users create a D2D group in response to the beacon considered BCU. Then, the bid values of the buyer and seller cellular users are transmitted to the base station side. The BS uses an experience‐weighted attraction‐learning model to obtain accurate bidding prices. Lastly, an ask‐to‐mean‐value double auction model was utilized instead of accurate bid values. Extensive simulation results demonstrate that the suggested strategy delivers excellent auction efficiency and fair earnings for both seller and buyer users. In addition, we identified that the participating users accomplished truthful bidding and used fewer resource blocks than existing techniques.
Skogea, a replacement name for Actinostephanus F. Wen, Y. G. Wei, and L. F. Fu (Gesneriaceae: Didymocarpoidae)
Authors: Deshmukh Umakant B. 
Published: March 2024

AbstractA replacement generic name, Skogea U. B. Deshmukh, is proposed for the name of the recently described genus Actinostephanus F. Wen, Y. G. Wei, and L. F. Fu (Gesneriaceae: Didymocarpoidae) segregated from Boeica C.B. Clarke, Rhynchotechum Blume, and Leptoboea Benth. because the name Actinostephanus has been recommended to be treated as a later homonym of Actinostephanos Khursevich (Bacillariophyta) by the nomenclature committee for algae.
Upregulation of the secretory pathway Ca2+/Mn2+‐ATPase isoform 1 in LPS‐stimulated microglia and its involvement in Mn2+‐induced Golgi fragmentation
Authors: Bhojwani‐Cabrera Aysha M. , Bautista‐García Alicia , Neubrand Veronika E. , Membrive‐Jiménez Francisco A. , Bramini Mattia , Martin‐Oliva David , Cuadros Miguel A. , Marín‐Teva José Luis , Navascués Julio , Vangheluwe Peter , Sepúlveda M. Rosario 
Published: March 2024

AbstractMicroglia play an important protective role in the healthy nervous tissue, being able to react to a variety of stimuli that induce different intracellular cascades for specific tasks. Ca2+ signaling can modulate these pathways, and we recently reported that microglial functions depend on the endoplasmic reticulum as a Ca2+ store, which involves the Ca2+ transporter SERCA2b. Here, we investigated whether microglial functions may also rely on the Golgi, another intracellular Ca2+ store that depends on the secretory pathway Ca2+/Mn2+‐transport ATPase isoform 1 (SPCA1). We found upregulation of SPCA1 upon lipopolysaccharide stimulation of microglia BV2 cells and primary microglia, where alterations of the Golgi ribbon were also observed. Silencing and overexpression experiments revealed that SPCA1 affects cell morphology, Golgi apparatus integrity, and phagocytic functions. Since SPCA1 is also an efficient Mn2+ transporter and considering that Mn2+ excess causes manganism in the brain, we addressed the role of microglial SPCA1 in Mn2+ toxicity. Our results revealed a clear effect of Mn2+ excess on the viability and morphology of microglia. Subcellular analysis showed Golgi fragmentation and subsequent alteration of SPCA1 distribution from early stages of toxicity. Removal of Mn2+ by washing improved the culture viability, although it did not effectively reverse Golgi fragmentation. Interestingly, pretreatment with curcumin maintained microglia cultures viable, prevented Mn2+‐induced Golgi fragmentation, and preserved SPCA Ca2+‐dependent activity, suggesting curcumin as a potential protective agent against Mn2+‐induced Golgi alterations in microglia.
Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing
Authors: Liu Yanan , Sun Shixian , Zhang Yanan , Song Miaomiao , Tian Yunyun , Lockhart Peter J. , Zhang Xin , Xu Ying , Dang Zhenhua 
Published: March 2024

AbstractBackgroundLong‐term overgrazing has led to severe degradation of grasslands, posing a significant threat to the sustainable use of grassland resources.MethodsBased on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing, moderate grazing, and heavy grazing treatments, the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses.ResultsDifferentially expressed genes and proteins were identified under different grazing intensities. They were mainly related to RNA processing, carbon metabolism, and secondary metabolite biosynthesis. These findings suggest that long‐term grazing leads to molecular phenotypic plasticity, affecting various biological processes and metabolic pathways in S. breviflora. Correlation analysis revealed low correlation between the transcriptome and the proteome, indicating a large‐scale regulation of gene expression at the posttranscriptional and translational levels during the response of S. breviflora to grazing. The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S. breviflora.ConclusionsOur study provides insight into the adaptation mechanisms of S. breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices.
Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
Authors: Komorowski Lukasz , Dabkowska Agnieszka , Madzio Joanna , Pastorczak Agata , Szczygiel Kacper , Janowska Martyna , Fidyt Klaudyna , Bielecki Maksymilian , Hunia Jaromir , Bajor Malgorzata , Stoklosa Tomasz , Winiarska Magdalena , Patkowska Elzbieta , Firczuk Malgorzata 
Published: March 2024

AbstractBreakpoint cluster region‐Abelson (BCR::ABL1) gene fusion is an essential oncogene in both chronic myeloid leukemia (CML) and Philadelphia‐positive (Ph+) B‐cell acute lymphoblastic leukemia (B‐ALL). While tyrosine kinase inhibitors (TKIs) are effective in up to 95% of CML patients, 50% of Ph+ B‐ALL cases do not respond to treatment or relapse. This calls for new therapeutic approaches for Ph+ B‐ALL. Previous studies have shown that inhibitors of the thioredoxin (TXN) system exert antileukemic activity against B‐ALL cells, particularly in combination with other drugs. Here, we present that peroxiredoxin‐1 (PRDX1), one of the enzymes of the TXN system, is upregulated in Ph+ lymphoid as compared to Ph+ myeloid cells. PRDX1 knockout negatively affects the viability of Ph+ B‐ALL cells and sensitizes them to TKIs. Analysis of global gene expression changes in imatinib‐treated, PRDX1‐deficient cells revealed that the nonhomologous end‐joining (NHEJ) DNA repair is a novel vulnerability of Ph+ B‐ALL cells. Accordingly, PRDX1‐deficient Ph+ B‐ALL cells were susceptible to NHEJ inhibitors. Finally, we demonstrated the potent efficacy of a novel combination of TKIs, TXN inhibitors, and NHEJ inhibitors against Ph+ B‐ALL cell lines and primary cells, which can be further investigated as a potential therapeutic approach for the treatment of Ph+ B‐ALL.
Anti‐inflammatory potential of remimazolam: A laboratory and clinical investigation
Authors: Tsukimoto Shota , Kitaura Atsuhiro , Kuroda Hidetaka , Imaizumi Uno , Yoshino Fumihiko , Yoshida Ayaka , Nakao Shinchi , Ohta Noriyuki , Nakajima Yasuhumi , Sanuki Takuro 
Published: March 2024

AbstractBackgroundAnesthetic agents, particularly intravenous anesthetics, may affect immune function and tumorigenic factors. We herein investigated whether the anti‐inflammatory effects of anesthetic agents are attributed to their antioxidant properties. The antioxidant and anti‐inflammatory effects of remimazolam, a new anesthetic, remain unclear. We hypothesized that remimazolam exerts anti‐inflammatory effects due to its antioxidant properties, which may affect the postoperative inflammatory response. This retrospective clinical study examined this hypothesis using laboratory and clinical approaches.MethodsThe antioxidant effects of remimazolam and dexmedetomidine were assessed by electron spin resonance (ESR) spectroscopy, and postoperative inflammatory responses were compared in 143 patients who underwent transcatheter aortic valve replacement at Kindai University Hospital between April 2021 and December 2022. The primary endpoint was the presence or absence of the antioxidant effects of the anesthetics themselves using ESR.ResultsRemimazolam at clinical concentrations exerted antioxidant effects, whereas dexmedetomidine did not. Increases in C‐reactive protein (CRP) levels on POD3 from preoperative values were significantly smaller in the remimazolam group than in the dexmedetomidine group (1.33 ± 1.29 vs. 2.17 ± 1.84, p = .014).ConclusionsRemimazolam exerted stronger anti‐inflammatory effects than dexmedetomidine, and these effects were enhanced by its antioxidant properties, which may have affected postoperative CRP production.
Maternal screening for sexually transmitted infections in pregnant patients in low‐resource facilities: A call to action
Authors: Kway Venance Basil , Castillo Reyther Roberto Arturo , Rios Sauceda Cynthia Guadalupe , Castro Martinez Ilse Veronica 
Published: March 2024

SynopsisASSURED criteria for POC test evaluation and rapid test analysis for STIs are discussed for cost‐effective screening, especially in low‐resource settings. Universal antepartum screening is advocated, with rapid tests as a viable option in low‐resource settings.
Association of long noncoding RNA H19 rs2839698 C/T with hepatitis B virus infection and hepatocellular carcinoma risk
Authors: Motawi Tarek K. , Mady Amira E. , Elhelbawy Mostafa , Talaat Roba M. , Shahin Nancy N. 
Published: March 2024

AbstractThe intricate pathogenesis of the hepatitis B virus (HBV) and its progression to hepatocellular carcinoma (HCC) have not yet been fully elucidated. H19 is one of the earliest imprinted long noncoding RNAs (lncRNAs) associated with liver pathobiology. This study investigated the association of H19 single nucleotide polymorphisms (SNPs) rs2839698 C/T and rs217727 C/T with HBV and HBV‐related HCC and their correlation with H19 expression level. A total of 230 subjects were enrolled in this study including 100 HBV‐infected patients, 30 HBV‐related HCC patients, and 100 apparently healthy controls. TaqMan genotyping human assays were utilized to assess allelic discrimination for H19 SNPs. H19 expression was assessed using quantitative real‐time polymerase chain reaction (qRT‐PCR). Our findings showed that H19 rs2839698 was linked to a higher incidence of HBV infection and HBV‐related HCC. Individuals who bear the CT genotype of rs2839698 were more susceptible to HBV infection (OR = 3.05; 95% CI 1.714–5.457; p < 0.001). Those harboring the TT genotype were more prone to develop HCC (OR = 2.625; 95% CI 1.037–6.64; p = 0.038). Our data revealed that rs2839698 could function as a promising predictor of HCC risk. Furthermore, H19 was significantly downregulated in HBV (p < 0.01) and HCC (p < 0.01) patients versus the control group. Significant upregulation of H19 in HCC patients with cirrhosis (p < 0.001) was detected. Altogether, this is considered the first prospective case‐control study to address the implication of the genetic variations of H19 SNPs in HBV and HBV‐related HCC in Egyptian patients.
HIF‐1α in cartilage homeostasis, apoptosis, and glycolysis in mice with steroid‐induced osteonecrosis of the femoral head
Authors: Yu Yaling , Jiang Yixin , Ge Hongfan , Fan Xiaoli , Gao Hang , Zhou Zhenlei 
Published: March 2024

AbstractWith the prevalence of coronavirus disease 2019, the administration of glucocorticoids (GCs) has become more widespread. Treatment with high‐dose GCs leads to a variety of problems, of which steroid‐induced osteonecrosis of the femoral head (SONFH) is the most concerning. Since hypoxia‐inducible factor 1α (HIF‐1α) is a key factor in cartilage development and homeostasis, it may play an important role in the development of SONFH. In this study, SONFH models were established using methylprednisolone (MPS) in mouse and its proliferating chondrocytes to investigate the role of HIF‐1α in cartilage differentiation, extracellular matrix (ECM) homeostasis, apoptosis and glycolysis in SONFH mice. The results showed that MPS successfully induced SONFH in vivo and vitro, and MPS‐treated cartilage and chondrocytes demonstrated disturbed ECM homeostasis, significantly increased chondrocyte apoptosis rate and glycolysis level. However, compared with normal mice, not only the expression of genes related to collagens and glycolysis, but also chondrocyte apoptosis did not demonstrate significant differences in mice co‐treated with MPS and HIF‐1α inhibitor. And the effects observed in HIF‐1α activator‐treated chondrocytes were similar to those induced by MPS. And HIF‐1α degraded collagens in cartilage by upregulating its downstream target genes matrix metalloproteinases. The results of activator/inhibitor of endoplasmic reticulum stress (ERS) pathway revealed that the high apoptosis rate induced by MPS was related to the ERS pathway, which was also affected by HIF‐1α. Furthermore, HIF‐1α affected glucose metabolism in cartilage by increasing the expression of glycolysis‐related genes. In conclusion, HIF‐1α plays a vital role in the pathogenesis of SONFH by regulating ECM homeostasis, chondrocyte apoptosis, and glycolysis.
The presence of radiotoxic 210Po and 210Pb in Ukrainian wild medicinal plants and the assessment of related dose and cancer risk
Authors: Moniakowska A. , Konieczyński P. , Block K. , Lysiuk R. , Shapovalova N. , Strumińska‐Parulska D. 
Published: March 2024

AbstractHerbal plants are considered natural pharmaceuticals but also accumulate chemical elements and compounds at high levels. 210Po and 210Pb are highly radiotoxic and may cause carcinogenesis due to ionizing radiation. Thirteen of the most popular wild‐growing herbaceous plant species, mostly included in the European Pharmacopoeia, were collected across Ukraine. 210Po and 210Pb were determined in the medicinal plants, and their radiotoxicity and cancer risk were calculated. The results of 210Po activities measured in herbal raw material ranged from 2.28 Bq kg−1 dw (where dw is dry weight) in herb of common horsetail (Equisetum arvense L.) collected near Lviv to 37.7 Bq kg−1 dw in leaves of common birch (Betula pendula Roth.) from Rava Ruska. The activity concentrations of 210Pb varied from 0.44 Bq kg−1 dw in leaves of common birch from Starokostyantyniv to 28.4 Bq kg−1 dw in leaves of common birch from Mizhhirya. There were statistically significant differences between 210Po and 210Pb content in flowers, leaves, and aerial portions. The studies indicated that the estimated annual effective radiation dose from Ukrainian herbs consumption was low, ranging 5.00–82.6 µSv from 210Po and 0.56–35.8 µSv from 210Pb, while the cancer morbidity and mortality risk ranged from 10−4 to 10−6. The presented data indicated no radiological risk related to using herbal plant materials.
Genetic counselors' and community clinicians' implementation and perceived barriers to informed consent during pre‐test counseling for hereditary cancer risk
Authors: Capasso Alexandra , Nehoray Bita , Gorman Nicholas , Quinn Emily A. , Bucio Daiana , Blazer Kathleen R. 
Published: March 2024

AbstractAs demand for genetic cancer risk assessment (GCRA) continues to increase, so does the sense of urgency to scale up efforts to triage patients, facilitate informed consent, and order genetic testing for cancer risk. The National Society of Genetic Counselors outlines the elements of informed consent that should be addressed in a GCRA session. While this practice resource aims to improve health equity, research on how well the elements of informed consent are implemented in practice is lacking. This retrospective and prospective mixed‐methods study assessed how adequately the elements of informed consent are addressed during pre‐test GCRA among 307 community clinicians (CC) and 129 cancer genetic counselors (GC), and barriers they face to addressing these elements. Results revealed that more than 90% of both cohorts consistently addressed components of at least 5 of the 10 elements of informed consent during a pre‐test consultation. Technical aspects and accuracy of the test and utilization of test results were the most similarly addressed elements. Notably, GCs more often review the purpose of the test and who to test, general information about the gene(s), and economic considerations whereas CCs more often review alternatives to testing. Both cohorts reported psychosocial aspects of the informed consent process as the least adequately addressed element. Time constraints and patient‐related concerns were most often cited by both cohorts as barriers to optimal facilitation of informed consent. Additional barriers reported by CCs included provider lack of awareness, experience, or education, and availability of resources and institutional support. Findings from this study may contribute to the development of alternative delivery models that incorporate supplementary educational tools to enhance patient understanding about the utility of genetic testing, while helping to mitigate the barrier of time constraints. Equally important is the use of this information to develop continuing education tools for providers.
High Torque teno virus load and outcome of patients undergoing allogeneic hematopoietic cell transplantation
Authors: Srour Micha , Grenier Corentin , Magro Leonardo , Hober Didier , Yakoub‐Agha Ibrahim , Engelmann Ilka 
Published: March 2024

AbstractQuantification of Torque teno virus (TTV) load emerged as a marker of immunosuppression. Associations of TTV load with complications and survival after allogeneic hematopoietic cell transplantation (allo‐HCT) were controversial in published studies. In this prospective study, we aimed to identify factors influencing TTV load after allo‐HCT and to determine whether the TTV load is associated with complications or outcomes. Seventy allo‐HCT recipients were included. TTV DNA load was quantified in 469 plasma samples of 70 patients from Day (D) 15 before D120 after transplantation. The influence of transplant characteristics on TTV load and the associations of TTV load with viral infections, acute graft versus host disease, mortality, and relapse were analyzed. More than 80% of patients were TTV DNA positive from D30 after transplantation onwards. Median TTV load increased between D30 and D60 post‐transplantation. Patients with lymphoid malignancies had higher TTV load than those with myeloid malignancies. Myeloablative conditioning was associated with higher TTV loads. Patients with no measurable residual disease at transplant had higher TTV loads. High TTV load at D90 post‐transplantation was associated with lower overall survival and at D120 post‐transplantation was associated with higher relapse rate. In conclusion, TTV load at time points later than D90 after allo‐HCT may be useful to assess prognosis.