bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced proapoptotic effects of tumor necrosis factor–related apoptosis-inducing ligand on temozolomide-resistant glioma cells
Authors: Uzzaman Mahmud , Keller Gordon , Germano Isabelle M. 
Published: January 2008

Object
Death receptor targeting is an attractive approach in experimental treatment for tumors such as malignant gliomas, which are resistant to radiation and chemotherapy. Among the family of cytokines referred to as death li gands, tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) has attracted clinical interest. The aim of this study was to assess whether TRAIL can be used as an adjuvant to temozolomide (TMZ) for apoptosis induction in malignant glioma cell lines.

Methods
Six human malignant glioma cell lines (A172, U87, U251, T98, U343, and U373) were exposed to human (h)TRAIL, TMZ, or an hTRAIL/TMZ combined treatment. Cell viability was assayed using 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide and phase-contrast microscopy. Cell apoptosis was detected using the terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling technique and quantified using flow cytometric analysis. The apoptosis signaling cascade was studied with Western blotting.
The additive effects of hTRAIL and TMZ resulted in a significant decrease in cell viability and an increased apoptotic rate. Expression of the death receptors DR5 and DR4 in two cell lines (A172 and U251) upregulated significantly when they were used in combination hTRAIL/TMZ treatment (p < 0.05 compared with baseline control), leading to activation of caspase-8 and caspase-3 (p < 0.05 compared with baseline control) and confirming an extrinsic apoptotic pathway. A cell intrinsic pathway through mitochondrial cytochrome c was not activated.

Conclusions
Based on this work, one may infer that hTRAIL should be considered as an adjuvant treatment for TMZ-resistant human malignant gliomas.
The surgical anatomy of ulnar and median nerve communications in the palmar surface of the hand
Authors: Loukas Marios , Louis Robert G. , Stewart Lynsey , Hallner Barry , DeLuca Terry , Morgan Walter , Shah Ranjil , Mlejnek Jim 
Published: January 2008

Object
Sensation in the palmar surface of the digits is supplied by the median and ulnar nerves, with the boundary classically being the midline of the ring finger. Overlap and variations of this division exist, and a communicating branch between the ulnar and median nerve could potentially explain further variations in digital sensory innervations. The aim of this study was to examine the origin and distribution of the communicating branch between the ulnar and median nerves and to apply such findings to the risk involved in surgical procedures in the hand.

Methods
The authors grossly and endoscopically examined 200 formalin-fixed adult human hands obtained in 100 cadavers, and a communicating branch was found to be present in 170 hands (85%). Of the specimens with communicating branches, the authors were able to identify four notable types representing different points of connections of the branches. The most common, Type I (143 hands, 84.1%), featured a communicating branch that originated proximally from the ulnar nerve and proceeded distally to join the median nerve. Type II (12 hands, 7.1%) designated a communicating branch that originated proximally from the median nerve and proceeded distally to join the ulnar nerve. Type III (six hands, 3.5%) designated a communicating branch that traversed perpendicularly between the median and ulnar nerves in such a way that it was not possible to determine which nerve served as the point of origin. Type IV (nine hands, 5.3%) designated a mixed type in which multiple communicating branches existed, arising from both ulnar and median nerves.

Conclusions
According to the origin and distribution of these branching patterns, the investigators were able to define a risk area in which the communicating branch(es) may be subject to iatrogenic injury during common hand procedures.
Chronic stimulation of the posterior hypothalamic region for cluster headache: technique and 1-year results in four patients
Authors: Starr Philip A. , Barbaro Nicholas M. , Raskin Neil H. , Ostrem Jill L. 
Published: January 2008

Object
Cluster headache (CH) is the most severe of the primary headache disorders. Based on the finding that regional cerebral blood flow is increased in the ipsilateral posterior hypothalamic region during a CH attack, a novel neurosurgical procedure for CH was recently introduced: hypothalamic deep brain stimulation (DBS). Two small case series have been described. Here, the authors report their technical approach, intraoperative physiological observations, and 1-year outcomes after hypothalamic DBS in four patients with medically intractable CHs.

Methods
Patients underwent unilateral magnetic resonance (MR) imaging–guided stereotactic implantation of a Medtronic DBS (model 3387) lead and Soletra pulse generator system. Intended tip coordinates were 3 mm posterior, 5 mm inferior, and 2 mm lateral to the midcommissural point. Microelectrode recording and intraoperative test stimulation were performed. Lead locations were measured on postoperative MR images. The intensity, frequency, and severity of headaches throughout a 1-week period were tracked in patient diaries immediately prior to surgery and after 1 year of continuous stimulation.
At the 1-year follow-up examination, DBS had produced a greater than 50% reduction in headache intensity or frequency in two of four cases. Active contacts were located 3 to 6 mm posterior to the mammillothalamic tract. Neurons in the target region showed low-frequency tonic discharge.

Conclusions
In two previously published case series, headache relief was obtained in many but not all patients. The results of these open-label studies justify a larger, prospective trial but do not yet justify widespread clinical application of this technique.
Dysphonia and dysphagia after anterior cervical decompression
Authors: Tervonen Hanna , Niemelä Mika , Lauri Eija-Riitta , Bäck Leif , Juvas Anja , Räsänen Pirjo , Roine Risto P. , Sintonen Harri , Salmi Tapani , Vilkman Erkki , Aaltonen Leena-Maija 
Published: January 2008

Object.
In this paper, the authors investigate the effects of anterior cervical decompression (ACD) on swallowing and vocal function.

Methods.
The study comprised 114 patients who underwent ACD. The early group (50 patients) was examined immediately pre- and postoperatively, and the late group (64 patients) was examined at only 3 to 9 months postoperatively. Fifty age- and sex-matched patients from the Department of Otorhinolaryngology—Head and Neck Surgery who had not been intubated in the previous 5 years were used as a control group. All patients in the early and control groups were examined by a laryngologist; patients in the late group were examined by a laryngologist and a neurosurgeon. Videolaryngostroboscopy was performed in all members of the patient and control groups, and the function of the ninth through 12th cranial nerves were clinically evaluated. Data were collected concerning swallowing, voice quality, surgery results, and health-related quality of life. Patients with persistent dysphonia were referred for phoniatric evaluation and laryngeal electromyography (EMG). Those with persistent dysphagia underwent transoral endoscopic evaluation of swallowing function and videofluorography.

Results.
Sixty percent of patients in the early group reported dysphonia and 69% reported dysphagia at the immediate postoperative visit. Unilateral vocal fold paresis occurred in 12%. The prevalence of both dysphonia and dysphagia decreased in both groups 3 to 9 months postoperatively. All six patients with vocal fold paresis in the early group recovered, and in the late group there were two cases of vocal fold paresis. The results of laryngeal EMG were abnormal in 14 of 16 patients with persistent dysphonia. Neither intraoperative factors nor age or sex had any effect on the occurrence of dysphonia, dysphagia, or vocal fold paresis. Most patients were satisfied with the surgical outcome.

Conclusions.
Dysphonia, dysphagia, and vocal fold paresis are common but usually transient complications of ACD. Recurrent laryngeal nerve damage detected by EMG is not rare. Pre-and postoperative laryngeal examination of ACD patients should be considered.
Spinal cord injury–induced expression of the antiangiogenic endostatin/collagen XVIII in areas of vascular remodelling
Authors: Mueller Christian A. , Conrad Sabine , Schluesener Hermann J. , Pietsch Torsten , Schwab Jan M. 
Published: January 2008

Object.
Spinal cord injury (SCI) induces the disruption of neural and vascular structures. In contrast to the emerging knowledge of mechanisms regulating the onset of the postinjury angiogenic response, little is known about counterregulatory signals.

Methods.
Using immunohistochemical methods, the authors investigated the expression of the endogenous angiogenic inhibitor endostatin/collagen XVIII during the tissue remodeling response to SCI.

Results.
After SCI, endostatin/collagen XVIII+ cells accumulated at the lesion site, in pannecrotic regions (especially in areas of cavity formation), at the lesion margin/areas of ongoing secondary damage, and in perivascular Virchow–Robin spaces. In remote areas (> 0.75 cm from the epicenter) a more modest accumulation of endostatin/collagen XVIII+ cells was observed, especially in areas of pronounced Wallerian degeneration. The numbers of endostatin/collagen XVIII+ cells reached their maximum on Day 7 after SCI. The cell numbers remained elevated in both, the lesion and remote regions, compared with control spinal cords for 4 weeks afterwards. In addition to being predominantly confined to ED1+-activated microglia/macrophages within the pannecrotic lesion core, endostatin/collagen XVIII expression was frequently detected by the endothelium/vessel walls. Numbers of lesional endostatin/collagen XVIII+ endothelium/vessel walls were found to increase early by Day 1 postinjury, reaching their maximum on Day 3 and declining subsequently to enhanced (above control) levels 30 days after SCI.

Conclusions.
The authors detected that in comparison to the early expression of neoangiogenic factors, there was a postponed lesional expression of the antiangiogenic endostatin/collagen XVIII. Furthermore, the expression of endostatin/collagen XVIII was localized to areas of neovascular pruning and retraction (cavity formation). The expression of endostatin/collagen XVIII by macrophages in a “late” activated phagocytic mode suggests that this factor plays a role in counteracting the preceding “early” neoangiogenic response after SCI.
Thoracolumbar junction injuries after motor vehicle collision: are there differences in restrained and nonrestrained front seat occupants?
Authors: Inamasu Joji , Guiot Bernard H. 
Published: January 2008

Object
Motor vehicle collision (MVC) is one of the most common causes of thoracolumbar junction (TLJ) injury. Although there is little doubt that the use of seat belts reduces the incidence and severity of TLJ injury after MVC, the mechanism by which this is protective against TLJ injury for drivers and passengers is relatively unknown.

Methods
Thirty-nine patients with TLJ (T11–L2) injury who were front seat occupants of a four-wheeled vehicle at the time of MVC (frontal crash) were admitted between 2000 and 2004. The 39 patients were divided into two groups: 18 who had been restrained and 21 who had not been restrained at the time of the MVC. Patient demographics, including the mean Injury Severity Scale score, incidence of neurological deficit, level of TLJ injury, and type of TLJ injury according to the Denis classification were compared.

Results
The incidence of neurological deficit in the restrained group was significantly lower compared with the nonrestrained group (5.6% compared with 33.3%, p < 0.05). The incidence of flexion–distraction/fracture–dislocation injuries in the restrained group was also significantly lower (0.0% compared with 33.3% in the nonrestrained group, p < 0.01). The restrained group was significantly older (37.4 ± 3.6 years compared with 28.0 ± 2.5 years in the nonrestrained group, p < 0.05), but otherwise there were no significant differences between the two groups regarding the patients' demographic data.

Conclusions
It is likely that the high incidence of neurological deficit in the nonrestrained front seat motor vehicle occupants who had a TLJ injury was mostly due to the high incidence of flexion–distraction/fracture–dislocation injuries. This retrospective study indirectly shows the efficacy of three-point seat belt systems in reducing the severity of a TLJ injury after an MVC. Compression/burst fractures still occur in restrained front seat occupants, however, and elucidation of the injury mechanism of such axial loading fractures may be important to improve safety further for automobile occupants.
Effect of lower two-level anterior cervical fusion on the superior adjacent level
Authors: Park Dong-Hyuk , Ramakrishnan Prem , Cho Tai-Hyoung , Lorenz Eric , Eck Jason C. , Humphreys S. Craig , Lim Tae-Hong 
Published: January 2008

Object
Symptomatic multisegment disease is most common at the C5–6 and C6–7 levels, and two-level anterior cervical discectomy and fusion (ACDF) is performed most often at these levels. Therefore, it may be clinically important to know whether a C5–7 fusion affects the superior C4–5 segment. A biomechanical study was carried out using cadaveric cervical spine specimens to determine the effect of lower two-level anterior cervical fusion on intradiscal pressure and segmental motion at the superior adjacent vertebral level.

Methods
Five cadaveric cervical spine specimens were used in this study. The specimens were stabilized at T-1 and loaded at C-3 to 15° flexion, 10° extension, and 10° lateral bending before and after simulated two-level ACDF with plate placement at C5–7. Intradiscal pressure was recorded at the C4–5 level, and segmental motion was recorded from C-4 through C-7. Differences in mean intradiscal pressures were calculated and analyzed using a paired Student t-test. When the maximum calibrated intradiscal pressures were exceeded (“overshot”) during measurements, data from the specimens involved were analyzed using the motion data with a Student t-test. Values for pressure and motion obtained before and after simulated ACDF were compared.

Results
During flexion, the mean intradiscal pressure changes (± standard deviations) in the pre- and post-ACDF measurements were 1275 (± 225) mm Hg and 2475 (± 75) mm Hg, respectively (p < 0.05). When the results of pre-ACDF testing were compared with post-ACDF results, no significant difference was found in the mean changes in the intradiscal pressure during extension and lateral bending. The maximum calibrated intradiscal pressures were exceeded during the post-ACDF testing in four specimens in extension, three in flexion, and two in lateral bending. Comparison of pre- and post-ACDF data for all five specimens revealed significant differences in motion and intradiscal pressure (p < 0.05) during flexion, significant differences in motion (p < 0.05) but not in intradiscal pressure during extension, and significant differences in intradiscal pressure changes (p < 0.05) but not in motion during lateral bending.

Conclusions
Simulated C5–7 ACDF caused a significant increase in intradiscal pressure and segmental motion in the superior adjacent C4–5 level during physiological motion. The increased pressure and hypermobility might accelerate normal degenerative changes in the vertebral levels adjacent to the anterior cervical fusion.
Increasing pedicle screw anchoring in the osteoporotic spine by cement injection through the implant
Authors: Fransen Patrick 
Published: January 2008

✓Instrumented spinal fusion in patients with osteoporosis is challenging because of the poor bone quality and is complicated by an elevated risk of delayed hardware failure. The author treated two patients presenting with severe osteoporosis, spinal stenosis, and degenerative spondylolisthesis. He performed decompressive laminectomy, posterolateral fusion, and pedicle screw (PS) fixation involving screws with side openings that allow cement to be injected through the implant. The cement injection was conducted under fluoroscopic control without complications.
Although this technique needs validation in a larger population of patients, the author believes that the injection of cement through these PSs can be performed safely in carefully selected patients. This technique creates not only a vertebroplasty-like effect that strengthens the vertebral body but also provides the additional stability afforded by the immediate anchoring of the screw, which may allow a shorter-length construct, save mobile segments, and finally reduce the risk of hardware failure.
Idiopathic spinal cord herniation: diagnostic, surgical, and follow-up data obtained in five cases
Authors: Maira Giulio , Denaro Luca , Doglietto Francesco , Mangiola Annunziato , Colosimo Cesare 
Published: January 2008

✓ Idiopathic spinal cord herniation (ISCH) is a rare, although increasingly recognized, cause of myelopathy. It is the result of an anterior dural defect in the thoracic spine through which the spinal cord herniates. Surgical restoration of the herniated cord to its normal position is usually followed by significant improvement in patients' clinical status. Differing surgical techniques have been used to manage the dural defect.
In this report the authors discuss the cases of five patients (four women and one man) with ISCH treated during a 13-year period. Clinical and imaging findings in each patient are reported. Two different surgical techniques were used to treat this condition: dural defect enlargement in two cases and dural patch secured with stitches in three. The intra-and postoperative findings are discussed in relation to the two surgical techniques.
Based on the results and complications in these five cases, the authors now believe that ISCH should be treated, when feasible, by using a dural patch to close the dural defect at the site of the herniation.
Quality of life assessment in patients undergoing nucleoplasty-based percutaneous discectomy
Authors: Gerszten Peter C. , Welch William C. , King Joseph T. 
Published: January 2008

ObjectNucleoplasty is a minimally invasive surgical procedure for disc decompression developed to treat patients with symptomatic contained herniated discs. Nucleoplasty uses nonheat-driven radiofrequency energy to ablate and coagulate the disc nucleus via a percutaneous “discography” trajectory under fluoroscopic guidance. In this study the authors evaluated pain, functioning, and quality of life (QOL) in patients with radicular leg and back pain who underwent nucleoplasty-based percutaneous disc decompression.MethodsThe study was designed as a prospective nonrandomized longitudinal cohort study in an academic medical center. Sixty-seven patients (mean age 41 years) with primarily radicular pain due to a contained disc herniation underwent nucleoplasty-based decompression in an outpatient setting. Patients completed the Medical Outcomes Study 36-Item Short Form (SF-36) Health Survey, EuroQol 5D (EQ5D), and a visual analog scale (VAS) for pain pre-operatively, and at 3 and 6 months after surgery. Postoperative QOL differences were assessed using the Wilcoxon signed-rank test. A surgical probe, the Perc-DLE SpineWand, was placed percutaneously into the disc after application of a local anesthetic or induction of general anesthesia to remove part of the disc (that is, a percutaneous discectomy).Nucleoplasty-treated levels were L2–3 (one case), L3–4 (five cases), L4–5 (44 cases), and L5–S1 (40 cases); there were 22 multiple treatment levels and 42 bilateral treatments. There were no infections or nerve root injuries associated with the procedure. Compared with preoperative QOL, there was a statistically significant improvement in QOL at 3 months as measured using the SF-36 Physical Component Summary (PCS) scale (mean score improvement 4.4 [p = 0.014]), the EQ5D (mean score improvement 0.22 [p = 0.001]), and the VAS for pain (mean score improvement 0.13 [p = 0.02]). Six-month results in 36 patients continued to reflect improvement as measured using the SF-36 PCS (mean score improvement 7.6 [p = 0.002]) and the EQ5D (mean score improvement 0.27 [p = 0.001]).ConclusionsNucleoplasty-based percutaneous disc decompression in patients with symptomatic contained disc herniations is safe and improves QOL as measured by the SF-36, EQ5D, and VAS for pain, three generic QOL outcome instruments. Nucleoplasty is an effective minimally invasive surgical treatment alternative in patients with symptomatic contained disc herniations. Further follow-up evaluation is underway to determine the durability of QOL improvement after nucleoplasty.
A comparison of adenosine A2A agonism and methylprednisolone in attenuating neuronal damage and improving functional outcome after experimental traumatic spinal cord injury in rabbits
Authors: Okonkwo David O. , Reece T. Brett , Laurent Jeffrey J. , Hawkins A. Stewart , Ellman Peter I. , Linden Joel , Kron Irving L. , Tribble Curtis G. , Stone James R. , Kern John A. 
Published: January 2008

Object
Steroid agents remain the lone pharmacological treatment in widespread use for acute spinal cord injury (SCI), although their utility remains in dispute in the neurotrauma literature. Adenosine A2A receptor activation with ATL-146e, a selective A2A agonist, has shown potential benefit in treating SCI; however, it has not been compared with the gold standard, methylprednisolone. The authors of this study evaluated ATL-146e and methylprednisolone for their ability to preserve neuronal viability and motor function in experimental SCI.

Methods
New Zealand White rabbits sustained SCI or sham injury via the Allen weight-drop technique. Ten minutes postinjury, animals received ATL-146e (ATL group, 0.06 μg/kg/min intravenously for 3 hours), methylprednisolone (steroid group, 30 mg/kg intravenously), or saline (trauma control group). Hindlimb motor function was recorded every 12 hours using the Tarlov motor grading scale (0, paralysis–5, normal hop). At 48 hours, fixed spinal cord tissue was evaluated for neuronal viability.
Hindlimb motor function in animals treated with ATL-146e was equivalent to that of sham-injured animals and was significantly better than that of trauma control animals at all time points and that of steroid-treated animals at 12 hours (p = 0.05). Motor function in steroid-treated animals was worse than in those given ATL-146e and better than that of trauma control animals at later time points, but was not statistically significant (both p > 0.05). Neuronal viability (measured in neurons/hpf) was significantly higher in both treatment groups compared with the trauma control group (12.1 ± 1.4 neurons/hpf for the ATL and 13.3 ± 1.4 neurons/hpf for the steroid group compared with 7.5 ± 1.5 neurons/hpf for the trauma control group; both p < 0.04). Neuronal viability did not differ among ATL-146e–treated, steroid-treated, and sham-injured groups.

Conclusions
The use of ATL-146e is at least as effective as methylprednisolone in preserving function and is equivalent to methylprednisolone in preserving the structure of spinal cord tissue after blunt SCI. Adenosine A2A receptor activation may be an effective treatment for acute SCI while avoiding the adverse effects of steroid agents.