bluuebunny/crossref_metadata_2025_split huggingface dataset:
Membrane‐initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells
Authors: Swiatek‐De Lange Magdalena , Stampfl Andreas , Hauck Stefanie M. , Zischka Hans , Gloeckner Christian J. , Deeg Cornelia A. , Ueffing Marius 
Published: June 2007

AbstractNeurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane‐initiated actions of progesterone in the retina and identified the membrane‐associated progesterone receptor component 1 (PGRMC1). We found PGRMC1 expressed mainly in retinal Muller glia (RMG) and retinal pigment epithelium, and localized uniquely to microsomal and plasma membrane fractions. In RMG, membrane‐impermeable progesterone conjugate induced calcium influx and subsequent phosphatidylinositol 3‐kinase‐mediated phosphorylation of PKC and ERK‐1/2. Induction by progesterone also led to PKC‐dependent activation ofVEGFgene expression and protein synthesis, suggesting a contribution of membrane‐initiated hormone effects to VEGF induced neovascularization within retina. © 2007 Wiley‐Liss, Inc.
Activation of PI3K‐Akt signaling pathway promotes prostate cancer cell invasion
Authors: Shukla Sanjeev , MacLennan Gregory T. , Hartman Douglas J. , Fu Pingfu , Resnick Martin I. , Gupta Sanjay 
Published: June 2007

AbstractActivated phosphoinositide 3‐kinase (PI3K) and its downstream target Akt/PKB are important signaling molecules and key survival factors involved in the control of cell proliferation, apoptosis and oncogenesis. We investigated the role of the PI3K‐Akt signaling pathway in the invasion of prostate cancer cell lines and activation of this pathway in primary human prostate tumors. Treatment of human prostate cancer cells viz. LNCaP, PC‐3 and DU145 with PI3K pharmacological inhibitor, LY294002, potentially suppressed the invasive properties in each of these cell lines. Restoration of the PTEN gene to highly invasive prostate cancer PC‐3 cells or expression of a dominant negative version of the PI3K target, Akt also significantly inhibited invasion and downregulated protein expression of urokinase‐type plasminogen activator (uPA) and matrix metalloproteinase (MMP)‐9, markers for cell invasion, indicating a central role of the PI3K‐Akt pathway in this process. Immunoblot analysis of PI3K and total/activated levels of Akt showed increased protein levels of catalytic (p110α/β) and regulatory (p85) subunits of PI3K and constitutive Akt activation in high‐grade tumors compared to low‐grade tumor and benign tissue. Immunohistochemical analyses further confirmed a progressive increase in p‐Akt (p‐Ser473) levels but not of total‐Akt (Akt1/2) in cancer tissues compared to benign specimens. A successive increase in p‐Akt expression was further noted in specimens serially obtained from individuals with time‐course disease progression. Taken together, these results suggest that aberrant activation of PI3K‐Akt pathway may contribute to increased cell invasiveness and facilitate prostate cancer progression. © 2007 Wiley‐Liss, Inc.
Pod1 induces myofibroblast differentiation in mesenchymal progenitor cells from mouse kidney
Authors: Plotkin Matthew , Mudunuri Vijay 
Published: June 2007

AbstractThe class II basic helix‐loop‐helix (bHLH) transcription factor Pod1 is expressed in mesenchymal cells including smooth muscle progenitors during development and in interstitial cells in adult organs. To determine the role of Pod1 in mesenchymal cell smooth muscle and myofibroblast differentiation, we examined a kidney progenitor cell line (4E) that endogenously expresses Pod1 and its class I bHLH partner E2A. In vitro‐translated Pod1 co‐immunoprecipitated E2A and increased E2A binding to a calponin promoter E‐box sequence as determined by an electrophoresis mobility shift assay (EMSA). Overexpression of Pod1 and E2A resulted in increased smooth muscle and myofibroblast gene expression including calponin, SM22α, αSMA, fibronectin, and connective tissue growth factor (CTGF) compared with overexpression of E2A alone. Suppression of Pod1 by siRNA resulted in increased cell proliferation and reduced expression of αSMA, fibronectin, and CTGF, and myofibroblast secreted proteins including pro‐fibrotic cytokines and inhibitors of matrix metalloproteinases. Examination of the signaling pathways for myofibroblast differentiation including Rho/Rho kinase and p38 MAPK showed that inhibition of actin polymerization by Rho kinase inhibitors decreased nuclear Pod1 levels while inhibition of p38 MAPK decreased Pod1 expression. These results indicate that Pod1 increases myofibroblast differentiation in combination with E2A and promotes a myofibroblast phenotype in mesenchymal progenitor cells. J. Cell. Biochem. 103: 675–690, 2008. © 2007 Wiley‐Liss, Inc.
Tip60 modulates PLAGL2‐mediated transactivation by acetylation
Authors: Ning Jinying , Zheng Gang , Yang Yu‐Chung 
Published: June 2007

AbstractPleiomorphic adenoma gene (PLAG) family proteins are oncogenes involved in various malignancies including lipoblastomas, hepatoblastomas, and acute myeloid leukemia. Overexpression of PLAGL2 induces cell transformation and proliferation, but little is known about how its activities are regulated. We previously showed that transcriptional activity of PLAGL2 is negatively regulated by sumoylation. Here we report that Tip60 modulates PLAGL2 functions through acetylation. Tip60 associates with PLAGL2 through its zinc finger domain and acetylates PLAGL2. Wild‐type but not the histone acetyltransferase (HAT)‐minus mutant form of Tip60 enhances PLAGL2‐mediated transactivation. In addition, coexpression of Tip60 and PLAGL2 completely abolishes the sumoylation of PLAGL2. Both Tip60 and DN‐Ubc9 increase transactivation activity of wild‐type but not the sumoylation deficient form of PLAGL2 (K250, 269, 356R), indicating that Tip60 acetylates PLAGL2 and abolishes the sumoylation of PLAGL2 possibly through modification of the same lysine residues (K250, 269, 356) within PLAGL2. Tip60 effects vary between different PLAGL2 target gene promoters, suggesting that Tip60 is a novel promoter‐specific coactivator of PLAGL2. This is the first demonstration that Tip60 can function as a sumoylation inhibitor in part through its intrinsic acetyltransferase activity to regulate specific gene expression. J. Cell. Biochem. 103: 730–739, 2008. © 2007 Wiley‐Liss, Inc.
Minimal molecular surfaces and their applications
Authors: Bates P. W. , Wei G. W. , Zhao Shan 
Published: June 2007

AbstractThis article presents a novel concept, the minimal molecular surface (MMS), for the theoretical modeling of biomolecules. The MMS can be viewed as a result of the surface free energy minimization when an apolar molecule, such as protein, DNA or RNA is immersed in a polar solvent. Based on the theory of differential geometry, the MMS is created via the mean curvature minimization of molecular hypersurface functions. A detailed numerical algorithm is presented for the practical generation of MMSs. Extensive numerical experiments, including those with internal and open cavities, are carried out to demonstrated the proposed concept and algorithms. The proposed MMS is typically free of geometric singularities. Application of the MMS to the electrostatic analysis is considered for a set of twenty six proteins. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008
Evidence‐based practice in clinical psychology: What it is, why it matters; what you need to know
Authors: Spring Bonnie 
Published: June 2007

AbstractThe history and meaning of evidence‐based practice (EBP) in the health disciplines was described to the Council of University Directors of Clinical Psychology (CUDCP) training programs. Evidence‐based practice designates a process of clinical decision‐making that integrates research evidence, clinical expertise, and patient preferences and characteristics. Evidence‐based practice is a transdisciplinary, idiographic approach that promotes lifelong learning. Empirically supported treatments (ESTs) are an important component of EBP, but EBP cannot be reduced to ESTs. Psychologists need additional skills to act as creators, synthesizers, and consumers of research evidence, who act within their scope of clinical expertise and engage patients in shared decision‐making. Training needs are identified in the areas of clinical trial methodology and reporting, systematic reviews, search strategies, measuring patient preferences, and acquisition of clinical skills to perform ESTs. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 611–631, 2007.
Evidence‐based practice in psychology: Implications for research and research training
Authors: Bauer Russell M. 
Published: June 2007

AbstractIn this article, the author discusses the implications of evidence‐based practice (EBP) for research and research training in clinical psychology. It is argued that EBP provides a useful framework for addressing some heretofore ignored problems in clinical research. Advancing evidence‐based psychological practice will require educators to inject significant new content into research, design, and methodology courses and to further integrate research and practicum training. The author believes this to be an exciting opportunity for the field, not only because it will further psychologists' integration into the interdisciplinary health care and research environment, but also because it will provide new tools to educate students for capable, not just competent professional activity. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 685–694, 2007.
Implementing elements of evidence‐based practice into scientist–practitioner training at the University of Nebraska‐Lincoln
Authors: DiLillo David , McChargue Dennis 
Published: June 2007

AbstractEvidence‐based practice (EBP) has become the predominant model of training and is emerging as a common model of practice for many nonpsychology health care professions. Recognizing the relevance of EBP to psychology, the American Psychological Association (APA) developed and endorsed an official policy statement on EBP for the practice of professional psychology. There is now a pressing need to consider ways that EBP can inform scientist–practitioner training. The present article proposes clinical competencies associated with the practice of EBP, and describes initial efforts to implement elements of EBP into training at the University of Nebraska‐Lincoln. These efforts have occurred in both the classroom and practicum training experiences, and are geared toward helping students become more effective users of the evidence base through their clinical work. Challenges to the implementation of EBP in clinical psychology training are discussed as well. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 671–684, 2007.
Barriers and facilitators of evidence‐based practice perceived by behavioral science health professionals
Authors: Pagoto Sherry L. , Spring Bonnie , Coups Elliot J. , Mulvaney Shelagh , Coutu Marie‐France , Ozakinci Gozde 
Published: June 2007

AbstractProgress in implementing evidence‐based behavioral practices has been slow. A qualitative study was performed to characterize the major facilitators and barriers to evidence‐based practice (EBP) perceived by behavioral professionals. Members of professional e‐mail listservs were queried and 84 barriers and 48 facilitators were nominated by 37 respondents. Thematic analysis revealed seven themes to describe both barriers and facilitators: (a) training, (b) attitudes, (c) consumer demand, (d) logistical considerations, (e) institutional support, (f) policy, and (g) evidence. Most frequently cited barriers included negative attitudes about EBP and lack of
training. Barriers also reflected confusion between EBP and the products of EBP (i.e., empirically supported treatments [ESTs]). Main facilitators included a growing evidence base. Results suggest that uptake of EBP may be facilitated by education and training. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 695–705, 2007.
Novel tools and resources for evidence‐based practice in psychology
Authors: Walker Barbara B. , London Susan 
Published: June 2007

AbstractGiven the vast proliferation of scientific research in the behavioral and social sciences, there is a growing need for psychologists to be able to access the most current, clinically relevant research quickly and efficiently and integrate this information into patient care. In response to a similar need within the field of medicine, evidence‐based medicine took hold in the early 1990s to provide both a framework and set of skills for translating research into practice. Since then, this method has been adopted by every major health care profession including psychology, and is now widely known as evidence‐based practice (EBP). In this article, the authors present a general overview of the skills required for EBP along with an introduction to some of the tools and resources that have been developed in other health care professions to support EBP. Using a case example, we illustrate how these tools and resources can be applied in psychological settings. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 633–642, 2007.
Development of a scale to measure adults' perceptions of health: Preliminary findings
Authors: Diamond James J. , Becker Julie A. , Arenson Christine A. , Chambers Christopher V. , Rosenthal Michael P. 
Published: June 2007

AbstractGiven the national agenda on chronic disease self‐management, the goal of the project described in this brief report was to develop a scale that measured adult perceptions about health but did not focus on a specific condition. The Perception of Health Scale (PHS) is based on earlier work that used the Health Belief Model as a focus. The 15‐item PHS was administered to 322 patients in several settings, and the responses were analyzed by the method of principal components. Four factors emerged that were named: Center of Control, Self‐Awareness, Certainty, and Importance of Health, each of which showed high internal consistency by Cronbach's α. Overall, the PHS is of sufficient internal consistency to make it a potentially useful tool in research designed to include a measure of personal beliefs about health. © 2007 Wiley Periodicals, Inc. J Comm Psychol 35: 557–561, 2007.
The synthesis of deuterium‐labeled spermine, N1‐acetyl‐spermine and N1‐acetylspermidine
Authors: Gawandi Vijay , Fitzpatrick Paul F. 
Published: June 2007

AbstractThe synthesis of deuterium‐labeled spermine, N1‐acetylspermine and N1‐acetylspermidine is reported. 1,1,3,3‐2H4‐N1‐Acetylspermine hydrochloride, 1,1,3,3‐2H4‐N1‐acetylspermidine hydrochloride and 1,1,3,3,10,10,12,12‐2H8‐spermine dihydrochloride were obtained in seven, four and three steps, respectively. All the syntheses were carried out by simple protection and deprotection steps from commonly used selective protecting reagents. These deuterium‐labeled compounds can be used as mechanistic probes of polyamine oxidizing enzymes. Copyright © 2007 John Wiley & Sons, Ltd.
Synergistic effect of low‐intensity pulsed ultrasound on growth factor stimulation of nucleus pulposus cells
Authors: Hiyama Akihiko , Mochida Joji , Iwashina Toru , Omi Hiroko , Watanabe Takuya , Serigano Kenji , Iwabuchi Sadahiro , Sakai Daisuke 
Published: June 2007

AbstractLow‐intensity pulsed ultrasound (LIPUS) has been reported to stimulate the activity of various cells. We have reported that the capacity of human intervertebral nucleus pulposus cell line to synthesize proteoglycan (PG) was increased by exposure to LIPUS, and postulated that one of the mechanisms underlying this response was an increase in expression of the transforming growth factor‐β type I receptor gene (TGFβR1). Therefore, the present study was conducted to assess the synergistic effect of LIPUS and TGF‐β on nucleus pulposus cells harvested from canines. The cells were cultured under four different sets of conditions: control group (Group A), LIPUS group (Group B), TGF‐β1 group (Group C), and LIPUS + TGF‐β1 group (Group D). They were evaluated by measuring cell proliferation, PG synthesis, PG content, gene expression of TGFβR1, and TGF‐β1 concentration. There were no significant differences in proliferation during culture. However, PG synthesis and endogenous TGF‐β1 production increased and demonstrated a synergistic effect between LIPUS and TGF‐β. Because LIPUS is safe and noninvasive, the results of the present study suggest that it would be a promising new therapy for prevention of intervertebral disc degeneration, which is said to be one of the primary causes of low back pain. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1574–1581, 2007
Dietary supplementation with polysaccharides from Semen cassiae enhances immunoglobulin production and interleukin gene expression in early‐weaned piglets
Authors: Deng Ze‐Yuan , Zhang Jin‐Wu , Wu Guo‐Yao , Yin Yulong , Ruan Zheng , Li Tie‐Jun , Chu Wu‐Ying , Kong Xiang‐Feng , Zhang You‐Ming , Fan Ya‐Wei , Liu Rong , Huang Rui‐Lin 
Published: June 2007

AbstractForty piglets were used to determine the effect of 14‐day dietary supplementation with polysaccharides from Semen cassiae (PSSA) on the immunity and interleukin (IL) gene expression in early‐weaned piglets. Piglets were weaned at 21 days of age and four pigs were euthanized to provide the data at initial point. The remaining 36 pigs were fed the following corn‐ and soybean meal‐based diets: the control diet (non‐PSSA), the low‐level PSSA diet (the control diet supplemented with 0.04% of PSSA), and the high‐level PSSA diet (the control diet supplemented with 0.08% PSSA). Blood samples were collected on days 0 and 14, and lymph nodes on day 14 of the experimental period for the following measurements: IL‐1β gene expression in blood mononuclear cells and jejunal mucosa; IL‐2 gene expression in lymphatic nodes; serum levels of cytokines (IL‐1β, IL‐2, and IL‐6); serum concentrations of immunoglobulins (IgA, IgG and IgM). Weaning stress resulted in decreases in serum antibody levels and cytoimmunity. Compared with the control group, dietary supplementation with a high level of PSSA increased: (1) serum concentrations of IL‐1β, IL‐2 and IL‐6, as well as IgG, IgA and IgM; (2) expression of the IL‐1β and IL‐2 genes in blood mononuclear cells and lymphatic nodes; and (3) expression of the IL‐1β gene in the jejunal mucosa. These novel findings suggest that polysaccharides from Semen cassiae enhance both the cell‐mediated immune response and the humoral immunity in the early‐weaned piglets. Copyright © 2007 Society of Chemical Industry
Preparation and properties of magnetic nano‐ and microsized particles for biological and environmental separations
Authors: Horák Daniel , Babič Michal , Macková Hana , Beneš Milan J. 
Published: June 2007

AbstractThe paper presents a critical overview on magnetic nanoparticles and microspheres used as separation media in different fields of chemistry, biochemistry, biology, and environment protection. The preparation of most widely used magnetic iron oxides in appropriate form, their coating or encapsulation in polymer microspheres, and functionalization is discussed in the first part. In the second part, new developments in the main application areas of magnetic composite particles for separation and catalytical purposes are briefly described. They cover separations and isolations of toxic inorganic and organic ions, proteins, and other biopolymers, cells, and microorganisms. Only selected number of relevant papers could be included due to the restricted extent of the review.
1H and 13C‐NMR data of hydroxyflavone derivatives
Authors: Park Younghee , Moon Byoung‐Ho , Lee Eunjung , Lee Youngshim , Yoon Youngdae , Ahn Joong‐Hoon , Lim Yoongho 
Published: June 2007

AbstractMany hydroxyflavone derivatives have been found in nature and shown to have many biological functions. Because their function is changed by the position and number of hydroxyl group, their structural identification is a fundamental and necessary step for understanding their functions. In the present study, the complete 1H and 13C NMR spectral assignments were presented for 6 hydroxyflavones, and NMR data of additional 14 hydroxyflavone derivatives were compared with those of the 6 hydroxyflavones. In addition, the partially incorrect NMR data of two of the dihydroxyflavones whose NMR data were previously reported were corrected. Copyright © 2007 John Wiley & Sons, Ltd.
The use of stochastic stresses in the static approach of probabilistic limit analysis
Authors: Alibrandi U. , Ricciardi G. 
Published: June 2007

AbstractIn the framework of the structural reliability theory, the probabilistic limit analysis (PLA) represents a powerful tool for the evaluation of the safety of structures with stochastic strengths with respect to the ultimate limit state of plastic collapse. Aim of the PLA is to evaluate the conditional probability of collapse (CPC), that is, the probability of plastic collapse of the structural system for assigned value of the acting loads. In this paper the focus is on the static approach of the limit analysis theory, which is particularly attractive for engineers, because it gives upper bounds of the CPC, that is, safe bounds. The classical static approach, introduced more than 30 years ago, however, cannot in any case evaluate the exact CPC of the structural system, and moreover generally the bounds obtained are not very close, especially in the range of very small probabilities. In this paper an alternative static approach is proposed, which can obtain the exact CPC of the structural
system considering a finite number of suitable chosen stochastic stress vectors through the use of the partial admissible domains; moreover, it gives good safe bounds of the CPC considering only a few stochastic stress vectors. Some simple numerical examples show the accuracy and effectiveness of the method. Copyright © 2007 John Wiley & Sons, Ltd.
Left‐sided gastroschisis and bilateral multicystic dysplastic kidneys: a rare combination of anomalies
Authors: Prasun Pankaj , Pradhan Mandakini , Kumari Niraj , Das Vinita 
Published: June 2007

AbstractLeft‐sided gastroschisis is very rare. We report a case of left‐sided gastroschisis associated with bilateral multicystic dysplastic kidneys. This combination of anomalies is unknown. The pathogenesis of gastroschisis is not well understood. It is now viewed as a malformation rather than disruption. The findings in this case support this view. The combination of dysplastic kidneys with ventral body wall defect suggests an early developmental defect. Copyright © 2007 John Wiley & Sons, Ltd.
Properties of a carbon filled cyclic olefin copolymer
Authors: Motlagh G. H. , Hrymak A. N. , Thompson M. R. 
Published: June 2007

AbstractThis article investigates electrical conductivity and rheological aspects of cyclic olefin copolymer (COC) composites containing both carbon fiber (CF) and carbon black (CB) at various concentrations. The different formulations of carbon filled COC were compression molded in such a manner that the formed circular sheets exhibited preferred in‐plane filler orientation. Through‐plane and in‐plane conductivity were measured by 2‐probe and 4‐probe methods, respectively, while an ARES rheometer in dynamic mode was employed to measure the storage modulus and complex viscosity. It was found that formulations with CF:CB ratios around 3 and where the CB content was close or below its critical percolation concentration resulted in higher electrical conductivity while maintaining the viscosity of the composite at a level acceptable for polymer processing machinery. For those composites containing both fillers, collaborative associations between the CB and CF fillers were found in the established percolating network structure, producing measured conductivities which exceeded the estimated values by the additive rule by up to sixfold. An empirical expression to handle hybrid filler systems is proposed in this work based on the standard percolation model. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 1808–1820, 2007