bluuebunny/crossref_metadata_2025_split huggingface dataset:
TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis
Authors: Wang Wenchao , Ma Zhenkai , Feng Xuemin , Ren Jiabin , Sun Shengyao , Shao Yuandong , Zhang Weimin , Yang Xiaoxia , Zhang Jiaming , Jing Xingzhi 
Published: March 2024

Abstract
Objective
Transferrin receptor-1 (TfR1) plays important roles in controlling cellular iron levels, but its role in OA pathology is unknown. Herein we aim to investigate the role of TfR1 in OA progression and its underlying mechanisms.

Methods
TfR1 expression in cartilage during OA development were examined both in vivo and in vitro. Then IL-1β was used to induce chondrocytes degeneration in vitro and TfR1 siRNA was used for observing the effect of TfR1 in modulating iron homeostasis, mitochondrial function and degrading enzymes expression. Also the inhibitor of TfR1 was exploited to analyze the protective effect of TfR1 inhibition in vivo.

Results
TfR1 is elevated in OA cartilage and contributes to OA inflammation condition. Excess iron not only results in oxidative stress damage and sensitizes chondrocytes to ferroptosis, but also triggers c-GAS/STING-mediated inflammation by promoting mitochondrial destruction and the release of mtDNA. Silencing TfR1 using TfR1 siRNA not only reduced iron content in chondrocytes and inhibited oxidative stress, but also facilitated the mitophagy process and suppressed mtDNA/cGAS/STING-mediated inflammation. Importantly, we also found that Ferstatin II, a novel and selective TfR1 inhibitor, could substantially suppress TfR1 activity both in vivo and in vitro and ameliorated cartilage degeneration.

Conclusion
Our work demonstrates that TfR1 mediated iron influx plays important roles in chondrocytes degeneration and OA pathogenesis, suggesting that maintaining iron homeostasis through the targeting of TfR1 may represent a novel therapeutic strategy for the treatment of OA.
Fulminant myocarditis during postoperative adjuvant chemotherapy for lung cancer with atezolizumab: a case report
Authors: Tokunaga Takuya , Aoki Masaya , Maruyama Koki , Nonaka Yuto , Kariatsumari Kota , Sakasegawa Koichi , Ueda Kazuhiro 
Published: March 2024

Abstract
Background
Postoperative adjuvant systemic therapy with atezolizumab for lung cancer has been reported to be effective. Although myocarditis is a rare immune adverse event associated with atezolizumab, it can have a serious course and should be treated with caution. We herein report a case of fulminant myocarditis during adjuvant systemic therapy with atezolizumab.

Case presentation
The patient was a 49-year-old Asian woman. She was diagnosed with pT2aN1M0 stage IIB (Programmed Death Ligand 1(PD-L1), 50%) after surgery for right upper lobe lung adenocarcinoma. Atezolizumab was administered following platinum-based adjuvant chemotherapy. On day 14, the patient was hospitalized because of deterioration in her general condition caused by fever. On day 16, she developed dyspnea, which worsened, and on day 17, she experienced shock. Blood tests, echocardiography, and cardiac catheterization were performed, and the patient was diagnosed with cardiogenic shock due to myocarditis. Initial measures did not improve the patient’s shock state. The patient was transferred to hospital for the use of an assistive circulatory system. Pulse steroid therapy was administered, and myocarditis showed a tendency toward improvement. A retrospective review of the patient’s history revealed a decreased lymphocyte count and an increase in the neutrophil/lymphocyte ratio, which may be useful for detecting severe immune-related adverse events. The troponin levels were elevated, but creatine phosphokinase level remained within the normal range.

Conclusion
Myocarditis can be fatal due to the rapid progression of symptoms. Close follow-up, a prompt diagnosis, and therapeutic intervention are important. Decreased lymphocyte counts, increased neutrophil/lymphocyte ratios, and the measurement of multiple myocardial biomarkers are considered useful for the early diagnosis of myocarditis.
O-GlcNAcylation: a pro-survival response to acute stress in the cardiovascular and central nervous systems
Authors: Xue Qiu , Ji Shengtao , Xu Hui , Yu Shu 
Published: March 2024

AbstractO-GlcNAcylation is a unique monosaccharide modification that is ubiquitously present in numerous nucleoplasmic and mitochondrial proteins. The hexosamine biosynthesis pathway (HBP), which is a key branch of glycolysis, provides the unique sugar donor UDP-GlcNAc for the O-GlcNAc modification. Thus, HBP/O-GlcNAcylation can act as a nutrient sensor to perceive changes in nutrient levels and trigger O-GlcNAc modifications of functional proteins in cellular (patho-)physiology, thereby regulating diverse metabolic processes. An imbalance in O-GlcNAcylation has been shown to be a pathogenic contributor to dysfunction in metabolic diseases, including type 2 diabetes, cancer, and neurodegeneration. However, under acute stress conditions, protein O-GlcNAc modification exhibits rapid and transient upregulation, which is strongly correlated with stress tolerance and cell survival. In this context, we discuss the metabolic, pharmacological and genetic modulation of HBP/O-GlcNAc modification in the biological system, the beneficial role of O-GlcNAcylation in regulating stress tolerance for cardioprotection, and neuroprotection, which is a novel and rapidly growing field. Current evidence suggests that transient activation of the O-GlcNAc modification represents a potent pro-survival signalling pathway and may provide a promising strategy for stress-related disorder therapy.
Contigs directed gene annotation (ConDiGA) for accurate protein sequence database construction in metaproteomics
Authors: Wu Enhui , Mallawaarachchi Vijini , Zhao Jinzhi , Yang Yi , Liu Hebin , Wang Xiaoqing , Shen Chengpin , Lin Yu , Qiao Liang 
Published: March 2024

Abstract
Background
Microbiota are closely associated with human health and disease. Metaproteomics can provide a direct means to identify microbial proteins in microbiota for compositional and functional characterization. However, in-depth and accurate metaproteomics is still limited due to the extreme complexity and high diversity of microbiota samples. It is generally recommended to use metagenomic data from the same samples to construct the protein sequence database for metaproteomic data analysis. Although different metagenomics-based database construction strategies have been developed, an optimization of gene taxonomic annotation has not been reported, which, however, is extremely important for accurate metaproteomic analysis.

Results
Herein, we proposed an accurate taxonomic annotation pipeline for genes from metagenomic data, namely contigs directed gene annotation (ConDiGA), and used the method to build a protein sequence database for metaproteomic analysis. We compared our pipeline (ConDiGA or MD3) with two other popular annotation pipelines (MD1 and MD2). In MD1, genes were directly annotated against the whole bacterial genome database; in MD2, contigs were annotated against the whole bacterial genome database and the taxonomic information of contigs was assigned to the genes; in MD3, the most confident species from the contigs annotation results were taken as reference to annotate genes. Annotation tools, including BLAST, Kaiju, and Kraken2, were compared. Based on a synthetic microbial community of 12 species, it was found that Kaiju with the MD3 pipeline outperformed the others in the construction of protein sequence database from metagenomic data. Similar performance was also observed with a fecal sample, as well as in silico mixed datasets of the simulated microbial community and the fecal sample.

Conclusions
Overall, we developed an optimized pipeline for gene taxonomic annotation to construct protein sequence databases. Our study can tackle the current taxonomic annotation reliability problem in metagenomics-derived protein sequence database and can promote the in-depth metaproteomic analysis of microbiome. The unique metagenomic and metaproteomic datasets of the 12 bacterial species are publicly available as a standard benchmarking sample for evaluating various analysis pipelines. The code of ConDiGA is open access at GitHub for the analysis of microbiota samples.
Manipulating the physical distance between cells during soil colonization reveals the importance of biotic interactions in microbial community assembly
Authors: Romdhane Sana , Huet Sarah , Spor Aymé , Bru David , Breuil Marie-Christine , Philippot Laurent 
Published: March 2024

AbstractBackgroundMicrobial communities are of tremendous importance for ecosystem functioning and yet we know little about the ecological processes driving the assembly of these communities in the environment. Here, we used an unprecedented experimental approach based on the manipulation of physical distance between neighboring cells during soil colonization to determine the role of bacterial interactions in soil community assembly. We hypothesized that experimentally manipulating the physical distance between bacterial cells will modify the interaction strengths leading to differences in microbial community composition, with increasing distance between neighbors favoring poor competitors.ResultsWe found significant differences in both bacterial community diversity, composition and co-occurrence networks after soil colonization that were related to physical distancing. We show that reducing distances between cells resulted in a loss of bacterial diversity, with at least 41% of the dominant OTUs being significantly affected by physical distancing. Our results suggest that physical distancing may differentially modulate competitiveness between neighboring species depending on the taxa present in the community. The mixing of communities that assembled at high and low cell densities did not reveal any “home field advantage” during coalescence. This confirms that the observed differences in competitiveness were due to biotic rather than abiotic filtering.ConclusionsOur study demonstrates that the competitiveness of bacteria strongly depends on cell density and community membership, therefore highlighting the fundamental role of microbial interactions in the assembly of soil communities.
Errors in visual search: Are they stochastic or deterministic?
Authors: Li Aoqi , Hulleman Johan , Wolfe Jeremy M. 
Published: March 2024

AbstractIn any visual search task in the lab or in the world, observers will make errors. Those errors can be categorized as “deterministic”: If you miss this target in this display once, you will definitely miss it again. Alternatively, errors can be “stochastic”, occurring randomly with some probability from trial to trial. Researchers and practitioners have sought to reduce errors in visual search, but different types of errors might require different techniques for mitigation. To empirically categorize errors in a simple search task, our observers searched for the letter “T” among “L” distractors, with each display presented twice. When the letters were clearly visible (white letters on a gray background), the errors were almost completely stochastic (Exp 1). An error made on the first appearance of a display did not predict that an error would be made on the second appearance. When the visibility of the letters was manipulated (letters of different gray levels on a noisy background), the errors became a mix of stochastic and deterministic. Unsurprisingly, lower contrast targets produced more deterministic errors. (Exp 2). Using the stimuli of Exp 2, we tested whether errors could be reduced using cues that guided attention around the display but knew nothing about the content of that display (Exp3a, b). This had no effect, but cueing all item locations did succeed in reducing deterministic errors (Exp3c).
Development of the China’s list of ambulatory care sensitive conditions (ACSCs): a study protocol
Authors: Wang Jianjian , Xu Dong Roman , Zhang Yan , Fu Hongqiao , Wang Sijiu , Ju Ke , Chen Chu , Yang Lian , Jian Weiyan , Chen Lei , Liao Xiaoyang , Xiao Yue , Wu Ruixian , Jakovljevic Mihajlo , Chen Yaolong , Pan Jay 
Published: March 2024

Abstract
Background
The hospitalization rate of ambulatory care sensitive conditions (ACSCs) has been recognized as an essential indicator reflective of the overall performance of healthcare system. At present, ACSCs has been widely used in practice and research to evaluate health service quality and efficiency worldwide. The definition of ACSCs varies across countries due to different challenges posed on healthcare systems. However, China does not have its own list of ACSCs. The study aims to develop a list to meet health system monitoring, reporting and evaluation needs in China.

Methods
To develop the list, we will combine the best methodological evidence available with real-world evidence, adopt a systematic and rigorous process and absorb multidisciplinary expertise. Specific steps include: (1) establishment of working groups; (2) generations of the initial list (review of already published lists, semi-structured interviews, calculations of hospitalization rate); (3) optimization of the list (evidence evaluation, Delphi consensus survey); and (4) approval of a final version of China’s ACSCs list. Within each step of the process, we will calculate frequencies and proportions, use descriptive analysis to summarize and draw conclusions, discuss the results, draft a report, and refine the list.

Discussion
Once completed, China’s list of ACSCs can be used to comprehensively evaluate the current situation and performance of health services, identify flaws and deficiencies embedded in the healthcare system to provide evidence-based implications to inform decision-makings towards the optimization of China’s healthcare system. The experiences might be broadly applicable and serve the purpose of being a prime example for nations with similar conditions.
Accelerated IVIM-corrected DTI in acute hamstring injury: towards a clinically feasible acquisition time
Authors: Rauh Susanne S. , Suskens Jozef J. M. , Monte Jithsa R. , Smithuis Frank , Gurney-Champion Oliver J. , Tol Johannes L. , Maas Mario , Nederveen Aart J. , Strijkers Gustav J. , Hooijmans Melissa T. 
Published: March 2024

Abstract
Background
Intravoxel incoherent motion (IVIM)-corrected diffusion tensor imaging (DTI) potentially enhances return-to-play (RTP) prediction after hamstring injuries. However, the long scan times hamper clinical implementation. We assessed accelerated IVIM-corrected DTI approaches in acute hamstring injuries and explore the sensitivity of the perfusion fraction (f) to acute muscle damage.

Methods
Athletes with acute hamstring injury received DTI scans of both thighs < 7 days after injury and at RTP. For a subset, DTI scans were repeated with multiband (MB) acceleration. Data from standard and MB-accelerated scans were fitted with standard and accelerated IVIM-corrected DTI approach using high b-values only. Segmentations of the injury and contralateral healthy muscles were contoured. The fitting methods as well as the standard and MB-accelerated scan were compared using linear regression analysis. For sensitivity to injury, Δ(injured minus healthy) DTI parameters between the methods and the differences between injured and healthy muscles were compared (Wilcoxon signed-rank test).

Results
The baseline dataset consisted of 109 athletes (16 with MB acceleration); 64 of them received an RTP scan (8 with MB acceleration). Linear regression of the standard and high-b DTI fitting showed excellent agreement. With both fitting methods, standard and MB-accelerated scans were comparable. Δ(injured minus healthy) was similar between standard and accelerated methods. For all methods, all IVIM-DTI parameters except f were significantly different between injured and healthy muscles.

Conclusions
High-b DTI fitting with MB acceleration reduced the scan time from 11:08 to 3:40 min:s while maintaining sensitivity to hamstring injuries; f was not different between healthy and injured muscles.

Relevance statement
The accelerated IVIM-corrected DTI protocol, using fewer b-values and MB acceleration, reduced the scan time to under 4 min without affecting the sensitivity of the quantitative outcome parameters to hamstring injuries. This allows for routine clinical monitoring of hamstring injuries, which could directly benefit injury treatment and monitoring.

Key points
• Combining high-b DTI-fitting and multiband-acceleration dramatically reduced by two thirds the scan time.
• The accelerated IVIM-corrected DTI approaches maintained the sensitivity to hamstring injuries.
• The IVIM-derived perfusion fraction was not sensitive to hamstring injuries.

Graphical Abstract
Shorter ZK-SNARKs from square span programs over ideal lattices
Authors: Lin Xi , Cao Heyang , Liu Feng-Hao , Wang Zhedong , Wang Mingsheng 
Published: March 2024

AbstractZero-knowledge succinct non-interactive arguments of knowledge (zk-SNARKs) are cryptographic protocols that offer efficient and privacy-preserving means of verifying NP language relations and have drawn considerable attention for their appealing applications, e.g., verifiable computation and anonymous payment protocol. Compared with the pre-quantum case, the practicability of this primitive in the post-quantum setting is still unsatisfactory, especially for the space complexity. To tackle this issue, this work seeks to enhance the efficiency and compactness of lattice-based zk-SNARKs, including proof length and common reference string (CRS) length. In this paper, we develop the framework of square span program-based SNARKs and design new zk-SNARKs over cyclotomic rings. Compared with previous works, our construction is without parallel repetition and achieves shorter proof and CRS lengths than previous lattice-based zk-SNARK schemes. Particularly, the proof length of our scheme is around $$23.3\%$$

23.3
%

smaller than the recent shortest lattice-based zk-SNARKs by Ishai et al. (in: Proceedings of the 2021 ACM SIGSAC conference on computer and communications security, pp 212–234, 2021), and the CRS length is $$3.6\times$$

3.6
×

smaller. Our constructions follow the framework of Gennaro et al. (in: Proceedings of the 2018 ACM SIGSAC conference on computer and communications security, pp 556–573, 2018), and adapt it to the ring setting by slightly modifying the knowledge assumptions. We develop concretely small constructions by using module-switching and key-switching procedures in a novel way.
Embryo response to aneuploidy through self-correction mechanism: a literature review
Authors: Harzif Achmad Kemal , Andyra Azizah Fitriayu , Sayogo Atikah , Ummah Nafi’atul , Puspawardani Aisyah Retno , Nurbaeti Putri , Wiweko Budi 
Published: March 2024

Abstract
Background
Meiotic and mitotic errors often lead to aneuploidy and mosaicism. In this context, the self-correction mechanism enables the embryo to preferentially retain and preserve euploid cells through processes such as apoptosis, necrosis, or marginalization. This mechanism is thought to minimize the chance of genetic abnormalities during cell development.

Materials and methods
A literature search for articles written in English from January 2013 to October 2023 was conducted on PubMed, EBSCO, and Scopus, using the keywords “self-correction,” “self-repair,” “aneuploidy,” “mosaicism,” and “embryo.”

Results
A total of 308 articles were collected, out of which 5 retrospective and 1 prospective study were selected based on inclusion criteria.

Discussions
Investigations showed that embryos remove chromosomally abnormal cells, supporting the self-correction mechanism. aCGH has been used in 4 studies to demonstrate the presence of self-correction in mosaic embryos. Furthermore, a higher relative viability of polyploidies than complex aneuploidies was observed, suggesting early discrimination against complex aneuploidy, particularly those arising from mitotic origins. However, there are doubts about the reliability of preimplantation genetic testing for aneuploidy at the blastocyst stage, as it may lead to a high rate of false positives and the discarding of "good" embryos.

Conclusions
Studies showed a self-correction mechanism in human embryos through the ability to expel abnormal cells. Further investigation is needed to elucidate the underlying mechanisms and determine optimal strategies for preimplantation genetic testing to fully understand and optimize the use of self-correction mechanisms in embryo assessment and selection.
Randomized pilot trial of cell phone support to improve medication adherence among adolescents and young adults with chronic health conditions
Authors: Sayegh Caitlin S. , MacDonell Karen K. , Iverson Ellen , Beard Breaon , Chang Nancy , Vu My H. , Belzer Marvin 
Published: March 2024

Abstract
Introduction
Adolescents and young adults (AYA) living with chronic medical conditions often struggle to develop medication adherence skills. This pilot trial evaluated the impact of a mobile health coaching intervention, Cell Phone Support (CPS), on medication adherence.

Methods
Interventions in this randomized trial were CPS delivered by phone calls (CPS-C), CPS delivered by text messages (CPS-T), or automated text message reminders (ATR). Participants were AYA with different chronic medical conditions (i.e., sickle cell disease, solid organ transplant, type 2 diabetes), aged 15–20 years (N = 34). We examined the feasibility, acceptability, and preliminary efficacy of each intervention.

Results
We examined the feasibility, acceptability, and preliminary efficacy of both CPS interventions. CPS was feasible and acceptable. There was evidence that participants found CPS to be more useful than ATR. In this pilot trial, participants receiving CPS reported relatively stronger increases in adherence, compared to those assigned to ATR. CPS-C slightly outperformed CPS-T.

Conclusions
Providing coaching to AYA struggling with illness self-management via their cell phones may promote their acquisition of medication adherence skills. Although larger studies are needed to confirm the results of this pilot study, phone calls and text messages are both promising modalities for delivering human cell phone support.

Trial registration
This trial was registered prospectively at ClinicalTrials.gov (NCT04241627) on 1/27/2020.