bluuebunny/crossref_metadata_2025_split huggingface dataset:
SIAH1 modulates antiviral immune responses by targeting deubiquitinase USP19
Authors: Weerawardhana Asela , Herath Thilina U. B. , Gayan Chathuranga W. A. , Kim Tae‐Hwan , Ekanayaka Pathum , Chathuranga Kiramage , Kang Ho‐Chul , Jung Jae U. , Lee Jong‐Soo 
Published: March 2024

AbstractTight control of the type I interferon (IFN) signaling pathway is critical for maintaining host innate immune responses, and the ubiquitination and deubiquitination of signaling molecules are essential for signal transduction. Deubiquitinase ubiquitin‐specific protein 19 (USP19) is known to be involved in deubiquitinating Beclin1, TRAF3, and TRIF for downregulation of the type I IFN signaling. Here, we show that SIAH1, a cellular E3 ubiquitin ligase that is involved in multicellular pathway, is a potent positive regulator of virus‐mediated type I IFN signaling that maintains homeostasis within the antiviral immune response by targeting USP19. In the early stages of virus infection, stabilized SIAH1 directly interacts with the USP19 and simultaneously mediates K27‐linked ubiquitination of 489, 490, and 610 residues of USP19 for proteasomal degradation. Additionally, we found that USP19 specifically interacts with MAVS and deubiquitinates K63‐linked ubiquitinated MAVS for negative regulation of type I IFN signaling. Ultimately, we identified that SIAH1‐mediated degradation of USP19 reversed USP19‐mediated deubiquitination of MAVS, Beclin1, TRAF3, and TRIF, resulting in the activation of antiviral immune responses. Taken together, these findings provide new insights into the molecular mechanism of USP19 and SIAH1, and suggest a critical role of SIAH1 in antiviral immune response and homeostasis.
UMP‐CMP kinase 2 inhibits ZIKV replication through activation of type I IFN signaling pathway
Authors: Zhu Ya , Tan Qi , Shi Yaoqiang , Li Qingyuan , Li Shilin , Wen Wenxian , Xie He , Li Bin , Duan Xiaoqiong , Chen Limin 
Published: March 2024

AbstractCytidine/uridine monophosphate kinase 2 (UMP‐CMP kinase 2, CMPK2) has been reported as an antiviral interferon‐stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, the association and the underlying mechanisms between CMPK2 induction and ZIKV replication remain to be determined. We investigated the induction of CMPK2 during ZIKV infection and the effect of CMPK2 on ZIKV replication in A549, U251, Vero, IFNAR‐deficient U5A and its parental 2fTGH cells, Huh7 and its RIG‐I‐deficient derivatives Huh7.5.1 cells. The activation status of Jak‐STAT signaling pathway was determined by detecting the phosphorylation level of STAT1, the activity of interferon stimulated response element (ISRE) and the expression of several interferon stimulated genes (ISGs). We found that ZIKV infection induced CMPK2 expression through an IFNAR and RIG‐I dependent manner. Overexpression of CMPK2 inhibited while CMPK2 knockdown promoted ZIKV replication in A549 and U251 cells. Mechanically, we found that CMPK2 overexpression increased IFNβ expression and activated Jak/STAT signaling pathway as shown by the increased level of p‐STAT1, enhanced activity of ISRE, and the upregulated expression of downstream ISGs. These findings suggest that ZIKV infection induced CMPK2 expression, which inhibited ZIKV replication and serves as a positive feedback regulator for IFN‐Jak/STAT pathway.
The Arabian Sea and Bay of Bengal play a key role in extreme precipitation during the snow season over the Tibetan Plateau
Authors: Wang Weiguo , Li Hongyi , Xie Zeming , Hao Xiaohua , Wang Jian 
Published: March 2024

AbstractWater vapour is a critical component of the hydrological cycle and plays a key role in climate systems and water resources on the Tibetan Plateau (TP). However, the effect of water vapour on extreme precipitation during the snow season over the TP, where snow cover is widespread, has not been determined. In this study, we investigated the water vapour contributions from extreme precipitation events during the snow season from 1980 to 2017 in four subregions of the TP using the flexible particle dispersion method. We divided the TP into four regions: the northeastern TP (NETP), northwestern TP (NWTP), southeastern TP (SETP) and southwestern TP (SWTP). The water vapour contributions from extreme precipitation among the four subregions were clearly different. The most important water vapour area that contributed the most water vapour to extreme precipitation over the NETP was from local contributions; nevertheless, Central Asia contributed the most water vapour to the NWTP. Water vapour from the Arabian Sea and Bay of Bengal (ASBB) dominantly controlled extreme precipitation in the SETP at a ratio of 45% and in the SWTP at a ratio of 72.9%. The water vapour contribution from the ASBB was significantly correlated with the extreme precipitation occurring in the TP subregions during the snow season in comparison to that of non‐extreme precipitation. These results could improve the understanding of water vapour cycle differences across the interior TP; additionally, our study provides a theoretical reference for the relationship between the snow distribution and water vapour sources in the TP.
Evaluation of the Prognosis of Patients With Acute Respiratory Distress Syndrome at the Emergency Department Based on the Lung Ultrasound Score
Authors: Altuğ Ertuğrul , Toksul İbrahim Halil , Çakir Adem , Şener Kemal , Korkut Semih , Kapci Mücahit , Güven Ramazan 
Published: March 2024

ObjectivesAcute respiratory distress syndrome (ARDS) is a respiratory disease characterized by a high rate of mortality. Determining the prognosis of this disease is therefore important. Lung ultrasonography has found increased use, especially in the recent years. This study aimed to score patients diagnosed with ARDS at the emergency department using point‐of‐care ultrasound (POCUS)‐Lung and to investigate the prognosis of patients with ARDS using a scoring system.MethodsThis study was designed as a single‐center prospective study. The study was performed in patients admitted to the emergency department and were diagnosed with ARDS pursuant to the Berlin criteria for ARDS and who met the inclusion criteria. The patients underwent lung ultrasonography at the emergency department and were scored (A line: 0; B1 line: 1; B2 line: 2; and C line: 3 points) accordingly.ResultsThe study included 100 patients with ARDS. The mortality rate was 52% in the patients in the study. The lung ultrasonography score in the mortality group (25.48 ± 3.64) was higher than that in the survivors (8.46 ± 3.61). For a cut‐off value of 17.5 for the lung ultrasonography score, the sensitivity and specificity with regard to mortality indicators were 92.8% and 90.9%, respectively (the area under the curve: 0.901; 95% confidence interval: 0.945–0.985: P < .001).ConclusionThe findings suggested that scoring based on POCUS‐Lung at the time of initial presentation at the emergency department in patients diagnosed with ARDS according to the Berlin criteria could help determine the prognosis. As POCUS‐Lung proved to be an important imaging method in investigating the affected alveolar capacity, we recommend its possible use as a prognostic indicator.
When the recipe is more important than the ingredients: Unveiling the complexity of consumer use of voice assistants
Authors: Heirati Nima , Pitardi Valentina , Temerak Mohamed Sobhy 
Published: March 2024

AbstractThe widespread use of voice assistants (VAs) creates a pressing need to understand what drives consumers to use different VAs. Existing studies have commonly focused on the net effects of antecedents that explain why consumers adopt or continue using VAs, ignoring the complexity of consumer behavior and the combinatorial effects of multiple antecedents. Our study proposes that consumer intention to continue using VAs does not depend on a single characteristic of products or consumers but on specific configurations of such characteristics. By integrating human–technology interaction and media richness theories, we suggest that consumers with distinct psychometric profiles and learning styles may evaluate humanlike and technological attributes of VAs differently. Our study shows that the complex interconnectedness between different VA attributes and consumer characteristics can provide a holistic understanding of why some consumers continue or stop using VAs. The results advance the media richness literature by offering novel insights into multimodality in consumer–technology interactions by examining consumer evaluations of single and multimodal VAs (e.g., smart speakers vs. touchscreen smart speakers). Our study provides templates for managers to effectively design VAs aligned with their segmentation and targeting strategies.
Ligand‐gated ion channels as potential biomarkers for ADT‐mediated cognitive decline in prostate cancer patients
Authors: Verma Shiv , Singh Vaibhav , Nagampalli VijayKrishna , Ponsky Lee E. , Li Chiang‐Shan R. , Chao Herta , Gupta Sanjay 
Published: March 2024

AbstractMen with prostate cancer are at increased risk of developing cognitive decline by the use of second‐generation androgen signaling inhibitors. To date, reliable and sensitive biomarkers that could distinguish men at high risk of cognitive dysfunction under androgen deprivation therapy (ADT) have not been characterized. We used high‐throughput transcriptional profiling utilizing human prostate cancer cell culture models mimicking ADT, biomarker selection using minimal common oncology data elements‐cytoscape, and bioinformatic analyses employing Advaita® iPathwayGuide and DisGeNET for identification of disease‐related gene associations. Validation analysis of genes was performed on brain neuronal and glial cells by quantitative real‐time polymerase chain reaction assay. Our systematic analysis of androgen deprivation‐associated genes involved multiple biological processes, including neuroactive ligand–receptor interaction, axon guidance, cytokine–cytokine receptor interaction, and metabolic and cancer signaling pathways. Genes associated with neuroreceptor ligand interaction, including gamma‐aminobutyric acid (GABA) A and B receptors and nuclear core proteins, were identified as top upstream regulators. Functional enrichment and protein–protein interaction network analysis highlighted the role of ligand‐gated ion channels (LGICs) and their receptors in cognitive dysfunction. Gene–disease association assigned forgetfulness, intellectual disability, visuospatial deficit, bipolar disorder, and other neurocognitive impairment with upregulation of type‐1 angiotensin II receptor, brain‐derived neurotrophic factor, GABA type B receptor subunit 2 (GABBR2), GABRA3, GABRA5, GABRB1, glycine receptor beta, glutamate ionotropic receptor N‐methyl‐D‐aspartate receptor (NMDA) type subunit 1, glutamate ionotropic receptor NMDA type subunit 2D, 5‐hydroxytryptamine receptor 1D, interferon beta 1, and nuclear receptor subfamily 3 group C member 1 as top differentially expressed genes. Validation studies of brain glial cells, neurons, and patients on ADT demonstrated the association of these genes with cognitive decline. Our findings highlight LGICs as potential biomarkers for ADT‐mediated cognitive decline. Further validation of these biomarkers may lead to future practical clinical use.
Enhancing healthy aging with small molecules: A mitochondrial perspective
Authors: Qin Xiujiao , Li Hongyuan , Zhao Huiying , Fang Le , Wang Xiaohui 
Published: March 2024

AbstractThe pursuit of enhanced health during aging has prompted the exploration of various strategies focused on reducing the decline associated with the aging process. A key area of this exploration is the management of mitochondrial dysfunction, a notable characteristic of aging. This review sheds light on the crucial role that small molecules play in augmenting healthy aging, particularly through influencing mitochondrial functions. Mitochondrial oxidative damage, a significant aspect of aging, can potentially be lessened through interventions such as coenzyme Q10, alpha‐lipoic acid, and a variety of antioxidants. Additionally, this review discusses approaches for enhancing mitochondrial proteostasis, emphasizing the importance of mitochondrial unfolded protein response inducers like doxycycline, and agents that affect mitophagy, such as urolithin A, spermidine, trehalose, and taurine, which are vital for sustaining protein quality control. Of equal importance are methods for modulating mitochondrial energy production, which involve nicotinamide adenine dinucleotide boosters, adenosine 5′‐monophosphate‐activated protein kinase activators, and compounds like metformin and mitochondria‐targeted tamoxifen that enhance metabolic function. Furthermore, the review delves into emerging strategies that encourage mitochondrial biogenesis. Together, these interventions present a promising avenue for addressing age‐related mitochondrial degradation, thereby setting the stage for the development of innovative treatment approaches to meet this extensive challenge.
Restoration of Intestinal Microbiota After Inulin Supplementation Halted: The Secondary Effect of Supplemented Inulin
Authors: An Ran , Zhou Xilong , Zhang Jing , Yang Yaqi , Lyu Chengang , Wang Dapeng 
Published: March 2024

ScopeConsumption of inulin could affect the intestinal microbiota composition. Hereby, it is aimed to investigate the intestinal microbial community restoration process when the inulin supplementation is terminated (i.e., the secondary effect).Methods and resultsThe current study investigates the response and restoration of intestinal microbiota to/after high (Inulin‐H) and low (Inulin‐L) dosage of inulin supplementation or sequential antibiotics and inulin (Anti‐Inulin‐L) supplementation, based on analysis of 16S rRNA gene sequences in C57BL/6 mice. The number of significantly changed genera in response to inulin is highest in Anti‐Inulin‐L (n = 66) group, followed by Inulin‐H (n = 51) and Inulin‐L (n = 38) group. After inulin supplementation stops, microbiota of all studied groups tend to recover to their original states, with highest percentage of inulin‐responding microbes stay significantly different at Anti‐Inulin‐L (93.94%) group, followed by Inulin‐H (74.51%) and Inulin‐L (44.12%) groups. Of note, the relative abundance of some non‐inulin‐responding taxa significantly increases during restoration.ConclusionSequential antibiotics and inulin supplementation induce greatest changes in the intestinal microbial composition, followed by high and low dosage of inulin. Additionally, the changes induce by supplemented inulin in the intestinal microbial community, provide a chance for some microbes to outcompete the other microbes during the spontaneous restoration.
What I see, what you say: How cross‐method variation sharpens characterization of irritability in early childhood
Authors: Parker Alyssa J. , Brock Peyton , Kryza‐Lacombe Maria , Briggs‐Gowan Margaret , Dougherty Lea R. , Wakschlag Lauren S. , Wiggins Jillian Lee 
Published: March 2024

AbstractObjectivesIdentification of clinically significant irritability in preschool age is important to implement effective interventions. However, varying informant and measurement methods display distinct patterns. These patterns are associated with concurrent and future mental health concerns. Patterns across multi‐informant methods in early‐childhood irritability may have clinical utility, identifying risk for impaired psychosocial functioning.MethodsUsing data from the Multidimensional Assessment of Preschoolers Study (N = 425), latent profile analysis identified irritability patterns through the parent‐reported Multidimensional Assessment Profile Scales–Temper Loss (MAPS‐TL), parent‐reported interviewer‐rated Preschool Age Psychiatric Assessment (PAPA), and observer‐rated Disruptive Behavior Diagnostic Observation Schedule (DB‐DOS). These profiles were characterized on protective factors, global functioning, and mental health syndromes, concurrently and at early school age and preadolescent follow‐up.ResultsFit indices favored a five‐class model: Low All, High Observation with Examiner (high DB‐DOS Examiner Context), High All, High Parent Report (high MAPS‐TL/PAPA), and Very High Parent Report (very high MAPS‐TL/PAPA). Whereas Low All and High Observation with Examiner exhibited strong psychosocial functioning, remaining profiles showed impaired psychosocial functioning, with the Very High Parent Report group showing higher impairment at follow‐ups,ds = 0.37–1.25.ConclusionsMulti‐informant measurements of irritability may have utility for clinical prediction, and future studies should test utility for diagnostic precision.
On the Polyak momentum variants of the greedy deterministic single and multiple row‐action methods
Authors: Wu Nian‐Ci , Zuo Qian , Wang Yatian 
Published: March 2024

AbstractFor solving a consistent system of linear equations, the classical row‐action method, such as Kaczmarz method, is a simple while really effective iteration solver. Based on the greedy index selection strategy and Polyak's heavy‐ball momentum acceleration technique, we propose two deterministic row‐action methods and establish the corresponding convergence theory. We show that our algorithm can linearly converge to a least‐squares solution with minimum Euclidean norm. Several numerical studies have been presented to corroborate our theoretical findings. Real‐world applications, such as data fitting in computer‐aided geometry design, are also presented for illustrative purposes.
Enhanced interfacial and tribological properties of fabric liner by constructing a MXene/HNTs/PAMAM hierarchical coating
Authors: Liu Meng , Zhang Zhaozhu , He Yaohui , Liao Chaoying , Yang Mingming , Chu Fanjie , Jiang Wei , Yuan Junya , Liu Weimin 
Published: March 2024

AbstractA special “hard‐soft” MXene/Halloysite nanotube (HNTs)/ poly(amidoamine) (PAMAM) structure was constructed to improve the interfacial and tribological performances of fabric liner (FL) materials. Thereinto, the MXene/HNTs coating were successfully grafted onto fabric surface by electrostatic self‐assembly strategy, and a novel PAMAM dendrimer polymer terminated by amino groups was prepared via condensation reaction. The mechanical and tribological performances of MXene/HNTs/PAMAM@FL as well as wear mechanism were systematically evaluated. Specifically, the incorporation of MXene/HNTs/PAMAM coating enhanced the bonding strength by 62.4%, and declined the wear rate by 27.7% in comparison with pure FL. The splendid performance was ascribed to the synergistic effect of “soft” toughening PAMAM polymer and “hard” lubricating MXene/HNTs hybrids. The introduction of MXene/HNTs hybrids could enhance mechanical interlocking and bearing capacity. Then PAMAM polymer ameliorated interfacial adhesion and compatibility with resin via forming chemical bonds, which further reducing stress concentration through increased crack deflections due to the steric hindrance.Highlights
MXene/HNTs/PAMAM coating was prepared via electrostatic self‐assembly and condensation reaction.
Interfacial adhesion of fabric liner achieved effective promotion.
High‐quality tribofilm was constructed by the synergistic enhancement effect between MXene/HNTs particles and PAMAM polymer.
The tribo‐chemical reactions participate in improving the lubricity and durability of tribofilm.
Enhancement of mechanical and thermal properties of UV‐curable wood coatings by cellulose nanofibrils and carboxylated organo‐montmorillonite
Authors: Sun Yuhui , Long Ling , Li Baojun , Kong Dingfeng , Liang Changli , He Ming , Liu Ru 
Published: March 2024

AbstractIn this study, the impact of incorporating cellulose nanofibrils (CNF) and carboxylated organo‐montmorillonite (OMMT), separately or in combination, into a UV‐curable wood coating was evaluated for performance enhancement. This study investigated the influence of nanoparticle loading percentage on the mechanical properties and thermal behavior of the coating, as well as the surface properties of digitally printed decorative wood‐based panels. The results indicated that the addition of CNF or OMMT increased the viscosity of the polymer solutions. Although CNF randomly oriented within the matrices, OMMT adhered to the surface of the polymer matrix. Chemical crosslinking was observed between CNF/OMMT and the coating. The addition of CNF and OMMT improved the thermal stability of the neat UV curing coating. The tensile strength of nanocomposite films with a 6 wt% loading of CNF increased significantly from 7.63 MPa to 12.73 MPa compared to pure films. The reinforcing effect of CNF on the tensile performance was significantly better than that of OMMT. Adding CNF to the matte coating slightly improved the clarity of the printed decorative panels. When CNF and OMMT were added together in the coating, there was a synergistic enhancement in the low gloss and high pencil hardness of the panels.Highlights
Both CNF and OMMT were chosen to achieve performance improvements
A simple filling method was adopted for preparation of reinforced coating
The nanocomposite films exhibited fine thermal and mechanical properties
The better integrated property was obtained from the C + CNF3 + OMMT0.8 sample
The physicochemical interaction of nanofiller with polymer molecule existed
Modeling the high‐cycle fatigue crack initiation in short fiber reinforced polymers with notches
Authors: Pietrogrande Riccardo , Carraro Paolo Andrea , Zappalorto Michele , De Monte Matthias , Quaresimin Marino 
Published: March 2024

AbstractIn this work, a new approach is proposed for predicting the life to crack initiation in Short Fiber Reinforced Polymers (SFRPs) under cyclic loadings in the presence of notches, cut‐outs and stress concentrations. A multiscale model is presented, encompassing two length scales for correlating the applied loads to the local stress fields in the matrix. These are used for defining an effective stress for predicting the life to crack initiation, accounting for the simultaneous influence of the notch geometry, the fiber content, orientation and distribution. The criterion was successfully validated on a large set of experimental results.Highlights
A new model is proposed for predicting fatigue crack initiation in notched SFRPs
The model is based on a multiscale procedure
Prediction is based on local matrix stresses averaged in a structural volume
The model accounts for fiber orientation, distribution and notch geometry
Bullying prevention training for Chinese preservice teachers' efficacy and beliefs toward bullying
Authors: Zhu Qianyu , Wang Cixin 
Published: March 2024

AbstractBullying is prevalent in many schools worldwide. Providing bullying prevention training to preservice teachers can make a significant difference in preventing school bullying. There is a lack of training in bullying prevention for preservice teachers in China, and the effectiveness of such training for bullying prevention has not yet been established. The present study examined the effectiveness of bullying prevention training on antibullying self‐efficacy and incorrect beliefs about bullying among Chinese preservice teachers. A total of 139 preservice teachers (Mage = 20.76 years, SD = 0.80 years, ranging from 19 to 23 years; 90.6% women) were randomly assigned to the bullying prevention training (n = 73) or the control group (n = 66). Preservice teachers completed pre‐ and posttraining surveys. The participants in the treatment group completed a 5‐week bullying prevention training program (1.5 h per week). Measures of antibullying self‐efficacy and incorrect beliefs about bullying were used to evaluate the effectiveness of the bullying prevention training. Results indicated that the training increased preservice teachers' antibullying self‐efficacy but did not decrease their incorrect beliefs about bullying. Practical implications, limitations, and future directions of these findings are discussed.