bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleep deprivation increases the regularity of isometric torque fluctuations
Authors: Oliveira João H. , Santos Paulo , Pezarat-Correia Pedro , Vaz João R. 
Published: March 2024

AbstractThe regularity of the fluctuations present in torque signals represent the adaptability of the motor control. While previous research showed how it is affected by neuromuscular fatigue and ageing, the underlying mechanisms remain unclear. It is currently under debate whether these changes are explained by central or peripheral neuromuscular mechanisms. Here, we experimentally manipulated the sleep of thirteen young adults through a supervised 24 h-sleep deprivation protocol. This study aimed to investigate the effect of sleep deprivation on the regularity of torque fluctuations, and other standard torque-related outcomes (Peak Torque – PT – and Rate of Torque Development – RTD). The participants were asked to perform knee extension maximal voluntary contractions (MVC) and submaximal knee extensions at 40% of MVC for 30 s. PT and RTD were calculated from the MVC and the regularity of the torque fluctuations was determined on the submaximal task through Sample Entropy (SampEn). In addition, rate of perceived effort (RPE) was collected. We found no significant changes in PT and RTD. The regularity of torque fluctuations significantly increased (i.e., a decrease in SampEn) after 24 h-sleep deprivation (PRE = 1.76 ± 0.268, POS24 = 1.71 ± 0.306; p = 0.044). Importantly, we found a negative correlation between RPE and SampEn relative changes after sleep deprivation. This study brings new insights towards the understanding of the underlying mechanisms that explain changes in torque fluctuations, demonstrating that these changes are not limited to neuromuscular processes but are also likely to be affected by other domains, such as psychological profile, which can indirectly affect the neural drive to the muscles.
Age and growth analysis of Loliginidae squid paralarvae in the NW Spanish coast using statolith increment counts
Authors: García-Mayoral Elsa , Roura Álvaro , Pierce Graham J. , González Ángel F. 
Published: March 2024

AbstractKnowledge of recruitment to a fishery is particularly important in short-lived species, like most cephalopods, in which there is a complete turnover of individual every 1 or 2 years. In this study, age, body length, statolith length and growth rates of loliginid paralarvae (Alloteuthis media, A. subulata and Loligo vulgaris) were determined to evaluate the form of the growth curve and differences between species and hatching seasons. A total of 222 paralarvae collected in zooplankton samples along the Galician coast (NW Spain) were used to determine relationships between dorsal mantle length (DML), statolith length (SL) and the number of increments (NI)) deposited in the statolith. The paralarvae of L. vulgaris were between 1 and 35 days old, while both Alloteuthis species ranged in age from 1 to 54 days. Generalised additive models (GAMs) revealed non-linear growth in DML, with L. vulgaris exhibiting higher DML-at-age than Alloteuthis species. The best parametric model fit for these data was obtained using exponential regressions. The SL–NI relationship also differed between species, with L. vulgaris having larger statolith length at age. The paralarvae of L. vulgaris and A. media had two well-differentiated hatching peaks, one in late spring-early summer (beginning of the upwelling season) and another peak in late autumn (end of the upwelling season). Alloteuthis subulata had an earlier hatching peak in spring than in the other two loliginid species—and the second peak in autumn was barely visible, possibly because this species occurs further north in areas not sampled in autumn.
Assessment of Ecosystem Services Provided by Macrophytes in Southern Baltic and Southern Mediterranean Coastal Lagoons
Authors: Robbe Esther , Rogge Linda , Lesutienė Jūratė , Bučas Martynas , Schernewski Gerald 
Published: March 2024

AbstractThe ecological importance of macrophytes is well known and reflected in nature protection law, for example, as a key biological quality element. However, the socio-economic role, such as the impact of macrophyte presence on recreational activities, is often overlooked. The purpose of this study was to assess the human benefits (or ecosystem services) provided by macrophytes. We developed a list of 25 macrophyte ecosystem services and 79 assessment indicators based on expert knowledge and literature data. First, hypothetical scenarios of coastal lagoons were developed to assess the impact of different ecological states (i.e., macrophyte coverage) and management measures (i.e., fisheries) on the ecosystem service provision. Scenario assessments were carried out by stakeholder workshops and literature search. Second, the ecosystem service potential of submerged and emergent macrophyte habitats were assessed by macrophyte experts and literature data. Results showed that cultural services are most important in terms of the overall actual provision of ecosystem services (scenario assessment) but also showing highest potential of the hypothetical ecosystem service provision (habitat assessment). Highest overall potential is shown for reeds and tall forb communities (83 out of maximum 125), followed by seagrass beds (71) and seaweed communities (61). Our ecosystem service assessment approaches (i.e., scenario and habitat-based) using socio-cultural data (i.e., stakeholders and experts-based) and biophysical data (i.e., indicators-based) can serve as supportive tools for coastal management and policy implementation visualizing the benefits of macrophytes to humans.
Dynamics and Dependencies in Regional Collaboration for Biodiversity Restoration: Reflections from the Netherlands
Authors: Baumgarten Sabine , Aarts Noelle , Fliervoet Jan M. , Krabbenborg Lotte 
Published: March 2024

Abstract
Biodiversity restoration on a landscape level requires people with different backgrounds to connect and collaborate over an extended period of time. Hence, understanding how conservation and restoration goals are negotiated and achieved necessitates an understanding of the dynamics of the social fabric: the social networks and interactions that develop, underpin, and sustain collective action. This paper identifies patterns and factors that have contributed to constructive collaboration for biodiversity in the rural area of Ooijpolder-Groesbeek, which has been at the vanguard of nature and landscape development in the Netherlands. We conducted a historical analysis of the period between 1985 and 2022, based on a broad range of literature and interviews with key actors in the region. We provide a narrative account of the tipping points and the preceding processes that propelled the region to its current state. The emergence of these tipping points is analyzed through the lens of a conceptual framework on the dynamic interplay between practices, social interactions, events, and circumstances. Our findings reveal how an integrative landscape approach, the use of suitable boundary objects, and continuous network building and relation management across various levels have contributed to the success of the collective effort.
Physiological and immunological barriers in the lung
Authors: Kageyama Takahiro , Ito Takashi , Tanaka Shigeru , Nakajima Hiroshi 
Published: March 2024

AbstractThe lungs serve as the primary organ for respiration, facilitating the vital exchange of gases with the bloodstream. Given their perpetual exposure to external particulates and pathogens, they possess intricate protective barriers. Cellular adhesion in the lungs is robustly maintained through tight junctions, adherens junctions, and desmosomes. Furthermore, the pulmonary system features a mucociliary clearance mechanism that synthesizes mucus and transports it to the outside. This mucus is enriched with chemical barriers like antimicrobial proteins and immunoglobulin A (IgA). Additionally, a complex immunological network comprising epithelial cells, neural cells, and immune cells plays a pivotal role in pulmonary defense. A comprehensive understanding of these protective systems offers valuable insights into potential pathologies and their therapeutic interventions.
Phase field modelling and simulation of damage occurring in human vertebra after screws fixation procedure
Authors: Preve Deison , Lenarda Pietro , Bianchi Daniele , Gizzi Alessio 
Published: March 2024

AbstractThe present endeavour numerically exploits the use of a phase-field model to simulate and investigate fracture patterns, deformation mechanisms, damage, and mechanical responses in a human vertebra after the incision of pedicle screws under compressive regimes. Moreover, the proposed phase field framework can elucidate scenarios where different damage patterns, such as crack nucleation sites and crack trajectories, play a role after the spine fusion procedure, considering several simulated physiological movements of the vertebral body. Spatially heterogeneous elastic properties and phase field parameters have been computationally derived from bone density estimation. A convergence analysis has been conducted for the vertebra-screws model, considering several mesh refinements, which has demonstrated good agreement with the existing literature on this topic. Consequently, by assuming different angles for the insertion of the pedicle screws and taking into account a few vertebral motion loading regimes, a plethora of numerical results characterizing the damage occurring within the vertebral model has been derived. Overall, the phase field results confirm and enrich the current literature, shed light on the medical community, which will be useful in enhancing clinical interventions and reducing post-surgery bone failure and screw loosening. The proposed computational approach also investigates the effects in terms of fracture and mechanical behaviour of the vertebral-screws body within different metastatic lesions opening towards major life threatening scenarios.
Indirect: invertible and discrete noisy image rescaling with enhancement from case-dependent textures
Authors: Do Huu-Phu , Chen Yan-An , Do-Tran Nhat-Tuong , Hua Kai-Lung , Peng Wen-Hsiao , Huang Ching-Chun 
Published: March 2024

AbstractRescaling digital images for display on various devices, while simultaneously removing noise, has increasingly become a focus of attention. However, limited research has been done on a unified framework that can efficiently perform both tasks. In response, we propose INDIRECT (INvertible and Discrete noisy Image Rescaling with Enhancement from Case-dependent Textures), a novel method designed to address image denoising and rescaling jointly. INDIRECT leverages a jointly optimized framework to produce clean and visually appealing images using a lightweight model. It employs a discrete invertible network, DDR-Net, to perform rescaling and denoising through its reversible operations, efficiently mitigating the quantization errors typically encountered during downscaling. Subsequently, the Case-dependent Texture Module (CTM) is introduced to estimate missing high-frequency information, thereby recovering a clean and high-resolution image. Experimental results demonstrate that our method achieves competitive performance across three tasks: noisy image rescaling, image rescaling, and denoising, all while maintaining a relatively small model size.
Piezoelectric biosensors: shedding light on principles and applications
Authors: Skládal Petr 
Published: March 2024

AbstractThe three decades of experience with piezoelectric devices applied in the field of bioanalytical chemistry are shared. After introduction to principles and suitable measuring approaches, active and passive methods based on oscillators and impedance analysis, respectively, the focus is directed towards biosensing approaches. Immunosensing examples are provided, followed by other affinity sensing approaches based on hybridization of nucleic acids, aptamers, monitoring of enzyme activities, and detection of pathogenic microbes. The combination of piezosensors with cell lines and testing of drugs is highlighted, including mechanically active cells. The combination of piezosensors with other measuring techniques providing original hybrid devices is briefly discussed.
Graphical Abstract
Large-scale heavy precipitation over the Czech Republic and its link to atmospheric circulation in CORDEX regional climate models
Authors: Beranová Romana , Kyselý Jan 
Published: March 2024

AbstractThe study evaluates ability of regional climate models (RCMs) to reproduce relationships between large-scale heavy precipitation events (LHPEs) over the Czech Republic and atmospheric circulation. We use an ensemble of 32 RCM simulations with the 0.11° resolution from the Euro-CORDEX project, and compare the historical simulations (1951–2005) against observations from the E-OBS data set. A novel selection criterion for LHPEs is proposed, defining these as days with at least 70% of all grid boxes over a given area with precipitation amounts exceeding the 90th grid-specific percentile of the seasonal distribution of daily amounts. The association with atmospheric circulation is investigated through circulation types derived from sea level pressure using airflow indices (direction, strength and vorticity). The majority of the RCMs capture that the frequency of days with LHPEs is higher in winter than summer, but almost all underestimate the occurrence of LHPEs in both seasons. In winter, the observed LHPEs are connected mainly with cyclonic types and westerly supertype; the role of nonwesterly and cyclonic-nonwesterly supertypes is significant only in the eastern part, where the Atlantic influence is weaker. In summer, the importance of cyclonic and nonwesterly types in producing LHPEs increases compared to winter. The RCMs reasonably well reproduce these links, including differences between seasons and regions, if their ensemble mean is evaluated, but large variations occur among individual simulations mainly in summer. The importance of cyclonic vorticity is overestimated in the RCMs, while westerly advection of moist air plays a smaller role in models than in observations.
Molecular characterisation of a novel sadwavirus infecting cattleya orchids in Australia
Authors: Tran Nga T. , Campbell Paul R. , Crew Kathleen S. , Geering Andrew D. W. 
Published: March 2024

AbstractThe complete genome sequence of a novel sadwavirus infecting cattleya orchids in South East Queensland is described. Isometric virions of c. 27 nm diameter were observed in sap extracts viewed under a transmission electron microscope, and the genome sequence of this virus was determined by high-throughput sequencing. The viral genome consists of two RNA components, 5,910 and 4,435 nucleotides (nt) in length, each encoding a long polyprotein, with predicted cleavage sites at H/Y, E/G, Q/S, and Q/G for the RNA1 and T/G for the RNA2 translation products, respectively. RNA2 has an additional small ORF of 684 nt near the 3ʹ untranslated region. Phylogenetic analysis based on an amino acid sequence alignment of the Pro-Pol region suggested that this virus is most closely related to pineapple secovirus A, a member of the subgenus Cholivirus, but warrants classification as a member of a new species because it exhibited no more than 64% amino acid identity in pairwise sequence comparisons. Because of the prominent purple ringspots that were observed on the leaves of some of the plants, we propose the name “cattleya purple ringspot virus” for this virus (suggested species name: “Sadwavirus cattleyacola”).
Robots still outnumber humans in web archives in 2019, but less than in 2015 and 2012
Authors: Jayanetti Himarsha R. , Garg Kritika , Alam Sawood , Nelson Michael L. , Weigle Michele C. 
Published: March 2024

AbstractThe significance of the web and the crucial role of web archives in its preservation highlight the necessity of understanding how users, both human and robot, access web archive content, and how best to satisfy this disparate needs of both types of users. To identify robots and humans in web archives and analyze their respective access patterns, we used the Internet Archive’s (IA) Wayback Machine access logs from 2012, 2015, and 2019, as well as Arquivo.pt’s (Portuguese Web Archive) access logs from 2019. We identified user sessions in the access logs and classified those sessions as human or robot based on their browsing behavior. To better understand how users navigate through the web archives, we evaluated these sessions to discover user access patterns. Based on the two archives and between the three years of IA access logs (2012 vs. 2015 vs. 2019), we present a comparison of detected robots vs. humans and their user access patterns and temporal preferences. The total number of robots detected in IA 2012 (91% of requests) and IA 2015 (88% of requests) is greater than in IA 2019 (70% of requests). Robots account for 98% of requests in Arquivo.pt (2019). We found that the robots are almost entirely limited to “Dip” and “Skim” access patterns in IA 2012 and 2015, but exhibit all the patterns and their combinations in IA 2019. Both humans and robots show a preference for web pages archived in the near past.
„Emergency critical care“ in der Notaufnahme. Literaturübersicht und aktuelle Konzepte
Authors: Reindl Michael , Rovas Alexandros , Köhnke Richard , Bergrath Sebastian , Herrmann Tobias , Kegel Michael , Pin Martin , Kumle Bernhard , Bernhard Michael , Kümpers Philipp 
Published: March 2024

ZusammenfassungBis zu 10 % aller Notfallpatienten sind intensiv- oder überwachungspflichtig – Tendenz steigend. Im klinischen Alltag können jedoch nur wenige dieser Fälle unmittelbar auf eine Intensiv- oder Überwachungsstation verlegt werden. Aus diesem Grund besteht mitunter die Notwendigkeit einer verlängerten notfallmedizinischen Versorgung kritisch kranker Patient:innen in der Notaufnahme. Unter dem Motto „upstairs care, downstairs“ haben große Notaufnahmen im angloamerikanischen Raum hierfür die „emergency critical care units“ (ECCU) entwickelt. Dieses Konzept steht für eine frühe hochintensive Versorgung kritisch kranker Patient:innen innerhalb von Notaufnahmen mit dem Ziel einer bestmöglichen Stabilisierung und Allokation im Krankenhaus. Im Rahmen dieser Übersichtsarbeit sollen die bisherigen Daten zu ECCU genauer analysiert und auf die Situation in deutschen Notaufnahmen übertragen werden.
Neoadjuvant anthracycline-based (5-FEC) or anthracycline-free (docetaxel/carboplatin) chemotherapy plus trastuzumab and pertuzmab in HER2 + BC patients according to their TOP2A: a multicentre, open-label, non-randomized phase II trial
Authors: Ginzac Angeline , Molnar Ioana , Durando Xavier , Motte Rouge Thibault De La , Petit Thierry , D’hondt Véronique , Campone Mario , Bonichon-Lamichhane Nathalie , Venat Bouvet Laurence , Levy Christelle , Augereau Paule , Pistilli Barbara , Arsene Olivier , Jouannaud Christelle , Nguyen Suzanne , Cayre Anne , Tixier Lucie , Mahier Ait Oukhatar Céline , Nabholtz Jean-Marc , Penault-Llorca Frédérique , Mouret-Reynier Marie-Ange 
Published: March 2024

Abstract
Purpose
Previous studies have reported the benefit of dual HER2-targeting combined to neoadjuvant chemotherapy in HER2-amplified breast cancer (HER2 + BC). Moreover, besides the cardiac toxicity following their association to Trastuzumab, anthracyclines chemotherapy may not profit all patients. The NeoTOP study was designed to evaluate the complementary action of Trastuzumab and Pertuzumab, and the relevance of an anthracycline-based regimen according to TOP2A amplification status.

Methods
Open-label, multicentre, phase II study. Eligible patients were aged ≥ 18 with untreated, operable, histologically confirmed HER2 + BC. After centralized review of TOP2A status, TOP2A-amplified (TOP2A+) patients received FEC100 for 3 cycles then 3 cycles of Trastuzumab (8 mg/kg then 6 mg/kg), Pertuzumab (840 mg/kg then 420 mg/kg), and Docetaxel (75mg/m2 then 100mg/m2). TOP2A-not amplified (TOP2A-) patients received 6 cycles of Docetaxel (75mg/m2) and Carboplatin (target AUC 6 mg/ml/min) plus Trastuzumab and Pertuzumab. Primary endpoint was pathological Complete Response (pCR) using Chevallier’s classification. Secondary endpoints included pCR (Sataloff), Progression-Free Survival (PFS), Overall Survival (OS), and toxicity.

Results
Out of 74 patients, 41 and 33 were allocated to the TOP2A + and TOP2A- groups respectively. pCR rates (Chevallier) were 74.4% (95%CI: 58.9–85.4) vs. 71.9% (95%CI: 54.6–84.4) in the TOP2A + vs. TOP2A- groups. pCR rates (Sataloff), 5-year PFS and OS were 70.6% (95%CI: 53.8–83.2) vs. 61.5% (95%CI: 42.5–77.6), 82.4% (95%CI: 62.2–93.6) vs. 100% (95%CI: 74.1–100), and 90% (95%CI: 69.8–98.3) vs. 100% (95%CI: 74.1–100). Toxicity profile was consistent with previous reports.

Conclusion
Our results showed high pCR rates with Trastuzumab and Pertuzumab associated to chemotherapy. They were similar in TOP2A + and TOP2A- groups and the current role of neoadjuvant anthracycline-based chemotherapy remains questioned.

Trial registration number
NCT02339532 (registered on 14/12/14).
Identify risk factors affecting participation of Turkish women in mammography screening for breast cancer prevention
Authors: Bayrakçeken Esra , Yaralı Süheyla , Alkan Ömer 
Published: March 2024

Abstract
Purpose
Cancer screening is a public health intervention aiming to reduce cancer-caused deaths. This study aims to determine the factors affecting the mammography screening time among women aged 40–69.

Methods
The micro dataset obtained from the Türkiye Health Survey conducted by the Turkish Statistical Institute (TurkStat) in 2019 and 2022 was used in the present study. Stereotype logistic regression was used to determine the variables affecting mammography screening and period for breast cancer prevention in women in Türkiye.

Results
Given the results achieved from the analysis, it was found that factors such as age, marital status, general health condition, comorbidity, receiving psychosocial support, high blood lipid levels, and performing breast self-examinations affected women’s adherence to cancer screening programs.

Conclusion
Since adherence to mammography increases with age, it is recommended to pay importance to education for women approaching the age of mammography screening. Educated individuals are expected to have access to multiple sources of information as to cancer and to access this information more easily. In order to gain more insight into the recommended preventive measures and outcomes related to cancer, it is suggested to review policies, which will increase the educational level of women, and provide privileges in the field of education.