bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of a Primitive Brain–Derived Neural Stem Cell Population Based on Aldehyde Dehydrogenase Activity
Authors: Corti Stefania , Locatelli Federica , Papadimitriou Dimitra , Donadoni Chiara , Salani Sabrina , Del Bo Roberto , Strazzer Sandra , Bresolin Nereo , Comi Giacomo P. 
Published: November 2005

Abstract
Stem cells are undifferentiated cells defined by their ability to self-renew and differentiate to progenitors and terminally differentiated cells. Stem cells have been isolated from almost all tissues, and an emerging idea is that they share common characteristics such as the presence of ATP-binding cassette transporter G2 and high telomerase and aldehyde dehydrogenase (ALDH) activity, raising the hypothesis of a set of universal stem cell markers. In the present study, we describe the isolation of primitive neural stem cells (NSCs) from adult and embryonic murine neurospheres and dissociated tissue, based on the expression of high levels of ALDH activity. Single-cell suspension was stained with a fluorescent ALDH substrate termed Aldefluor and then analyzed by flow cytometry. A population of cells with low side scatter (SSClo) and bright ALDH (ALDHbr) activity was isolated. SSCloALDHbr cells are capable of self-renewal and are able to generate new neurospheres and neuroepithelial stem-like cells. Furthermore, these cells are multipotent, differentiating both in neurons and macroglia, as determined by immunocytochemistry and real-time reverse transcription–polymerase chain reaction analysis. To evaluate the engraftment potential of SSCloALDHbr cells in vivo, we transplanted them into mouse brain. Donor-derived neurons with mature morphology were detected in the cortex and subcortical areas, demonstrating the capacity of this cell population to differentiate appropriately in vivo. The ALDH expression assay is an effective method for direct identification of NSCs, and improvement of the stem cell isolation protocol may be useful in the development of a cell-mediated therapeutic strategy for neurodegenerative diseases.
A Crosstalk Between Myeloma Cells and Marrow Stromal Cells Stimulates Production of DKK1 and Interleukin-6: A Potential Role in the Development of Lytic Bone Disease and Tumor Progression in Multiple Myeloma
Authors: Gunn William G. , Conley Adam , Deininger Lisa , Olson Scott D. , Prockop Darwin J. , Gregory Carl A. 
Published: November 2005

Abstract
Multiple myeloma (MM) is a malignancy of antibody-secreting plasma cells. B-cell plasmacytomas stimulate bone resorption and angiogenesis, resulting in osteolytic lesions in the skeleton which persist upon successful treatment of the malignancy with chemotherapy. We found that an interaction between MM cells and mesenchymal stem cells (MSCs) from bone marrow stroma results in the formation and persistence of osteolytic bone lesions. It is known that MM cells activate osteoclast activity and secrete high levels of the Wnt inhibitor, Dickkopf-1, which prevents MSCs from differentiating into osteoblasts. We show that the Wnt signaling activator 6-bromoindirubin-3′-monoxime (BIO) releases MSCs from the osteoinhibitory effects of Dickkopf-1, whereas LiCl treatment does not. Additionally, we show that the &gt;5-kDa fraction of MSC-conditioned medium promotes the proliferation of Dickkopf-1-secreting MM cells and that an interleukin-6 (IL-6)-neutralizing antibody blocks this effect. IL-6 expression levels were higher in undifferentiated MSCs than in MSCs treated with osteogenic medium, remained high in the presence of Dkk1, and were reduced by BIO treatment. Therefore, BIO treatment reduces the MSC-stimulated proliferation of MM cells and may enable MSCs to repair existing osteolytic lesions.
Characterization of a New NIH-Registered Variant Human Embryonic Stem Cell Line, BG01V: A Tool for Human Embryonic Stem Cell Research
Authors: Plaia Todd W. , Josephson Richard , Liu Ying , Zeng Xianmin , Ording Carol , Toumadje Arazdordi , Brimble Sandii N. , Sherrer Eric S. , Uhl Elizabeth W. , Freed William J. , Schulz Thomas C. , Maitra Anirban , Rao Mahendra S. , Auerbach Jonathan M. 
Published: November 2005

Abstract
Human embryonic stem cells (hESCs) offer a renewable source of a wide range of cell types for use in research and cell-based therapies. Characterizing these cells provides important information about their current state and affords relevant details for subsequent manipulations. For example, identifying genes expressed during culture, as well as their temporal expression order after passaging and conditions influencing the formation of all three germ layers may be helpful for the production of functional beta islet cells used in treating type I diabetes. Although several hESC lines have demonstrated karyotypic instability during extended time in culture, select variant lines exhibit characteristics similar to their normal parental lines. Such variant lines may be excellent tools and abundant sources of cells for pilot studies and in vitro differentiation research in which chromosome number is not a concern, similar to the role currently played by embryonal carcinoma cell lines. It is crucial that the cells be surveyed at a genetic and proteomic level during extensive propagation, expansion, and manipulation in vitro. Here we describe a comprehensive characterization of the variant hESC line BG01V, which was derived from the karyotypically normal, parental hESC line BG01. Our characterization process employs cytogenetic analysis, short tandem repeat and HLA typing, mitochondrial DNA sequencing, gene expression analysis using quantitative reverse transcription-polymerase chain reaction and microarray, assessment of telomerase activity, methylation analysis, and immunophenotyping and teratoma formation, in addition to screening for bacterial, fungal, mycoplasma, and human pathogen contamination.
Sugar convertibility in the parasitoid Cotesia glomerata (Hymenoptera: Braconidae)
Authors: Hausmann Claudia , Wäckers Felix L. , Dorn Silvia 
Published: November 2005

AbstractLack of suitable sugar sources for adult parasitic wasps is an important cause of failure in biological control programs, but the metabolic constraints of sugar feeding are poorly understood. Here we investigated the suitability of 11 naturally occurring sugars as energy sources for the parasitoid Cotesia glomerata (L.) (Hymenoptera: Braconidae). By feeding energy‐deprived individuals with given quantities of a 40% (w:w) sugar solution, we assessed recovery time and lifespan after feeding. More than 50% of the wasps recovered within 20 min, and at least 80% within 60 min after uptake of one of the monosaccharides fructose or glucose, the disaccharide sucrose, or the trisaccharide melezitose. Between 40 to 80% of the test insects recovered within an hour after intake of maltose, raffinose, galactose, or mannose. Less than 25% recovered within 1 h after uptake of melibiose, trehalose, rhamnose, or water (control). Parasitoids obtained the highest lifespan benefits after intake of glucose, fructose, sucrose, or melezitose, indicating and confirming their convertibility as an energy source for C. glomerata. In contrast, no lifespan increase was found after consumption of rhamnose and trehalose. The differences in recovery time and lifespan are discussed in terms of parasitoid enzyme activity and metabolic processes. Arch. Insect Biochem. Physiol. 60:223–229, 2005. © 2005 Wiley‐Liss, Inc.
Host‐parasite interactions between whiteflies and their parasitoids
Authors: Gelman Dale B. , Gerling Dan , Blackburn Michael B. , Hu Jing S. 
Published: November 2005

AbstractThere is relatively little information available concerning the physiological and biochemical interactions between whiteflies and their parasitoids. In this report, we describe interactions between aphelinid parasitoids and their aleyrodid hosts that we have observed in four host‐parasite systems: Bemisia tabaci/Encarsia formosa, Trialeurodes vaporariorum/E. formosa, B. tabaci/Eretmocerus mundus, and T. lauri/Encarsia scapeata. In the absence of reported polydnavirus and teratocytes, these parasitoids probably inject and/or produce compounds that interfere with the host immune response and also manipulate host development to suit their own needs. In addition, parasitoids must coordinate their own development with that of their host. Although eggs are deposited under all four instars of B. tabaci, Eretmocerus larvae only penetrate 4th instar B. tabaci nymphs. A pre‐penetrating E. mundus first instar was capable of inducing permanent developmental arrest in its host, and upon penetration stimulated its host to produce a capsule (epidermal in origin) in which the parasitoid larva developed. T. vaporariorum and B. tabaci parasitized by E. formosa initiated adult development, and, on occasion, produced abnormal adult wings and eyes. In these systems, the site of parasitoid oviposition depended on the host species, occurring within or pressing into the ventral ganglion in T. vaporariorum and at various locations in B. tabaci. E. formosa's final larval molt is cued by the initiation of adult development in its host. In the T. lauri‐E. scapeata system, both the host whitefly and the female parasitoid diapause during most of the year, i.e., from June until the middle of February (T. lauri) or from May until the end of December (E. scapeata). It appears that the growth and development of the insects are directed by the appearance of new, young foliage on Arbutus andrachne, the host tree. When adult female parasitoids emerged in the spring, they laid unfertilized male‐producing eggs in whiteflies containing a female parasitoid [autoparasitism (development of male larvae utilizing female parasitoid immatures for nutrition)]. Upon hatching, these male larvae did not diapause, but initiated development, and the adult males that emerged several weeks later mated with available females to produce the next generation of parasitoid females. Thus, the interactions that exist between whiteflies and their parasitoids are complex and can be quite diverse in the various host‐parasitoid systems. Arch. Insect Biochem. Physiol. 60:209–222, 2005. Published 2005 Wiley‐Liss, Inc.
Expanded bed adsorption as a primary recovery step for the isolation of the insulin precursor MI3 process development and scale up
Authors: Brixius Peter , Mollerup Inger , Jensen Ole Elvang , Halfar Markus , Thömmes Jörg , Kula Maria‐Regina 
Published: November 2005

AbstractExpanded bed adsorption (EBA) was evaluated for the isolation of the human insulin precursor MI3, expressed and secreted by the yeast Saccharomyces cerevisiae. The isoelectric point of the insulin precursor (pH 5.3) makes cation exchange a prime candidate for direct adsorption. In order to find a suitable window of operation for the process the adsorption equilibrium was analysed in a wide range of operating conditions (pH and conductivity) and for three different stationary phases. The same array of operating conditions was examined with regard to stable fluidisation of the adsorbents in S. cerevisiae suspensions. Interactions of the yeast with the fluidised stationary phase were investigated by a pulse response technique and the hydrodynamics of the fluidised bed under process conditions by residence time distribution analysis. The case study demonstrates that by parallel examination of product binding and fluidisation quality a window of operation can be found. Analysis of the binding kinetics by breakthrough experiments and modelling led to the definition of a set of operating conditions, which yield a compromise between optimal use of the equilibrium capacity provided by the adsorbent and high throughput required for an industrial separation. After initial experiments on the bench scale the protocol was transferred successfully to pilot scale demonstrating the design of a reliable operation. © 2005 Wiley Periodicals, Inc.
Role of erythrocyte deformability during capillary wetting
Authors: Zhou Ronghui , Gordon Jason , Palmer Andre F. , Chang Hsueh‐Chia 
Published: November 2005

AbstractDeformability of erythrocyte was found to fundamentally alter the wetting dynamics of red blood cell (RBC) suspensions during their invasion into capillaries. Normal RBC suspensions failed to penetrate more than 1 cm into a glass capillary when the capillary radius was smaller than a critical value that is dependent on the erythrocyte concentration (about 50 µm for whole blood). In contrast, suspensions of rigidified RBCs, after cross‐linking with different concentrations of glutaraldehyde or incubating with 100 ng/mL of an endotoxin, could penetrate any capillary larger than the erythrocyte dimension. The effect of RBC deformability on penetration was attributed to the enhanced shear‐induced migration of normal deformable RBCs toward the capillary centreline, which imparted a higher average velocity to the RBCs than the average plasma velocity. As a result, the erythrocytes advanced into the capillary faster than the wetting meniscus, packing behind it to form a concentrated slug. This tightly packed slug had a high hydrodynamic resistance that could arrest the penetrating flow of concentrated suspensions into the small capillaries. © 2005 Wiley Periodicals, Inc.
High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification
Authors: Richter Sven , Nieveler Jens , Schulze Holger , Bachmann Till T. , Schmid Rolf D. 
Published: November 2005

AbstractThe mutant M301A of the acetylcholinesterase B from Nippostrongylus brasiliensis (NbAChE) was produced in a high‐cell‐density fermentation of a recombinant methylotrophic yeast Pichia pastoris. Dissolved oxygen (DO) spikes were used as an indicator for feeding the carbon source. Wet cell weight (WCW) reached after 8 days a maximum value of 316 g/L and the OD600 at this time was 280. The acetylcholinesterase activity increased up to 6,600 U/mL corresponding to an expression rate of 2 g of NbAChE per liter supernatant. The specific activity of the mutant NbAChE was determined after purification as 3,300 U/mg. Active site titration with chlorpyrifos, a strong AChE inhibitor, yielded in a specific activity of 3,400 U/mg. The enzyme was secreted by Pichia pastoris. Therefore, it could be concentrated from culture broth by cross‐flow‐filtration (50 kDa cut‐off membrane). It was further purified in one‐step anion‐exchange chromatography, using a XK 50/20 column filled with 125 mL Q Sepharose HP. Mutant NbAChE was purified 1.9‐fold up to a purity of 97% and a yield of 87%. The isolated enzyme was nearly homogenous, as seen on the silver stained SDS–PAGE as well as by a single peak after gel filtration. This extraordinary high expression rate and the ease of purification is an important prerequisite for their practical application, for example in biosensors for the detection of neurotoxic insecticides. © 2005 Wiley Periodicals, Inc.
Physiological studies of eicosapentaenoic acid production in the marine microalga Glossomastix chrysoplasta
Authors: Hsiao Tracy Y. , Blanch Harvey W. 
Published: November 2005

AbstractWe describe the characterization of the microalga Glossomastix chrysoplasta, an eicosapentaenoic acid (EPA) producer in the Pinguiophyceae class, Chromophyte division. Growth conditions were selected to optimize algal growth and EPA production. EPA represented up to 30% of the fatty acid content of Glossomastix chrysoplasta, at levels of 22 mg EPA per gram dry weight. Up to 72% of the EPA was produced as glycolipids, components of structural lipids. The optimal growth conditions in continuous culture were found to be greater than 500 µmol photons/m2 · s light intensity, 0.33/day dilution rate, pH 7.20–7.45, and a temperature of 18–20°C. Macronutrient studies indicated the limiting nutrient to be bicarbonate or dissolved carbon dioxide, and consequently decreasing pH increased EPA production. © 2005 Wiley Periodicals, Inc.
Structural Characterization of LnBaCo2O6‐δ Phases (Ln: Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho).
Authors: Anderson P. S. , Kirk C. A. , Knudsen J. , Reaney I. M. , West A. R. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Production of (R)‐Chiral Alcohols by a Hydrogen‐Transfer Bioreduction with NADH‐Dependent Leifsonia Alcohol Dehydrogenase (LSADH).
Authors: Inoue Kousuke , Makino Yoshihide , Itoh Nobuya 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Bromine Complexes of Polyvinylpyrrolidone Supports: A Mild Reagent for the Transformation of α‐Oxoketene Dithioacetals to β‐Oxothiolesters
Authors: Mathew Paulson , Nair Satheesh K. , Sreedhar K. M. , Pillai V. N. Rajasekharan , Asokan C. V. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Solvent‐Free Catalytic Friedel—Crafts Acylation of Aromatic Compounds with Carboxylic Acids by Using a Novel Heterogeneous Catalyst System: p‐Toluenesulfonic Acid/Graphite.
Authors: Hosseini Sarvari Mona , Sharghi Hashem 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Pd(CH3CN)2Cl2‐Catalyzed Oxidative Heterodimerization Reaction of 2,3‐Allenamides and 1,2‐Allenyl Ketones: An Efficient Synthesis of 4‐(Furan‐3′‐yl)‐2(5H)‐furanimines.
Authors: Ma Shengming , Gu Zhenhua , Yu Zhanqian 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Uncommon Heterocyclization into a Pyrazole System of Z‐3‐(2‐Naphthyl)‐3‐chloro‐2‐propenal Semicarbazone and Thiosemicarbazone.
Authors: Vashkevich E. V. , Potkin V. I. , Kozlov N. G. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Heterocycles Synthesis Based on Arylation Products of Unsaturated Compounds. Part 7. Reactions of 2‐Aryl‐1,4‐benzoquinones with Dithiol Compounds.
Authors: Obushak N. D. , Martyak R. L. , Matiichuk V. S. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 3‐(6‐R‐Benzothiazol‐2‐yl)‐4‐methyl‐1,2,5‐oxadiazoles.
Authors: Britsun V. N. , Borisevich A. N. , Samoilenko L. S. , Lozinskii M. O. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Palladium‐Assisted Multicomponent Synthesis of 2‐Aryl‐4‐aminoquinolines and 2‐Aryl‐4‐amino[1,8]naphthyridines.
Authors: Abbiati Giorgio , Arcadi Antonio , Canevari Valentina , Capezzuto Luca , Rossi Elisabetta 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
The Fluoride Ion Induced Intramolecular Conjugate Addition of Propargylsilanes to Dihydropyridones. A Novel Method for the Stereoselective Construction of Azabicyclic Ring Systems.
Authors: Dziedzic Magdalena , Lipner Grzegorz , Illangua Jose M. , Furman Bartlomiej 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
A Synthetic Route to 11‐(1H‐Pyrrol‐1‐yl)‐11H‐indeno[1,2‐b]quinoxaline Derivatives Exploiting a Three‐Component Coupling Strategy under Microwave Irradiation.
Authors: Azizian Javad , Karimi Ali Reza , Kazemizadeh Zahra , Mohammadi Ali A. , Mohammadizadeh Mohammad R. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
New Diterpenoids from Stems and Roots of Caesalpinia crista
Authors: Cheenpracha Sarot , Srisuwan Rattikan , Karalai Chatchanok , Ponglimanont Chanita , Chantrapromma Suchada , Chantrapromma Kan , Fun Hoong‐Kun , Anjum Shazia , Atta‐ur‐Rahman Atta‐ur‐Rahman 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Total Synthesis of HM‐1 and HM‐2, Aromatic Sesquiterpenes Isolated from the Phytopathogenic Fungus Helicobasidium mompa. Structure Revision of HM‐2.
Authors: Srikrishna A. , Ravikumar P. C. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
cis‐Dihydrodiol, Arene Oxide and Phenol Metabolites of Dictamnine: Key Intermediates in the Biodegradation and Biosynthesis of Furoquinoline Alkaloids.
Authors: Boyd Derek R. , Sharma Narain D. , O'Dowd Colin R. , Carroll Jonathan G. , Loke Pui L. , Allen Christopher C. R. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Total Synthesis of Buergerinin F via Effective Construction of the Asymmetric Quaternary Carbons Using an Enantioselective Aldol Reaction.
Authors: Shiina Isamu , Kawakita Yo‐ichi , Ibuka Ryoutarou , Yokoyama Kazutoshi , Yamai Yu‐suke 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Carbonic Anhydrase Inhibitors: Design of Thioureido Sulfonamides with Potent Isozyme II and XII Inhibitory Properties and Intraocular Pressure Lowering Activity in a Rabbit Model of Glaucoma.
Authors: Mincione Francesco , Starnotti Michele , Masini Emanuela , Bacciottini Lucia , Scrivanti Chiara , Casini Angela , Vullo Daniela , Scozzafava Andrea , Supuran Claudiu T. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of an N‐Acyl Sulfamate Analogue of Luciferyl‐AMP: A Stable and Potent Inhibitor of Firefly Luciferase.
Authors: Branchini Bruce R. , Murtiashaw Martha H. , Carmody Jill N. , Mygatt Emily E. , Southworth Tara L. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
2‐Aminoquinazoline Inhibitors of Cyclin‐Dependent Kinases.
Authors: Bathini Yadagiri , Singh Inderjit , Harvey Patricia J. , Keller Paul R. , Singh Rajeshwar , Micetich Ronald G. , Fry David W. , Dobrusin Ellen M. , Toogood Peter L. 
Published: November 2005

AbstractFor Abstract see ChemInform Abstract in Full Text.
Hormone complement of the Cancer productus sinus gland and pericardial organ: An anatomical and mass spectrometric investigation
Authors: Fu Qiang , Kutz Kimberly K. , Schmidt Joshua J. , Hsu Yun‐Wei A. , Messinger Daniel I. , Cain Shaun D. , de la Iglesia Horacio O. , Christie Andrew E. , Li Lingjun 
Published: November 2005

AbstractIn crustaceans, circulating hormones influence many physiological processes. Two neuroendocrine organs, the sinus gland (SG) and the pericardial organ (PO), are the sources of many of these compounds. As a first step in determining the roles played by hemolymph‐borne agents in the crab Cancer productus, we characterized the hormone complement of its SG and PO. We show via transmission electron microscopy that the nerve terminals making up each site possess dense‐core and/or electron‐lucent vesicles, suggesting diverse complements of bioactive molecules for both structures. By using immunohistochemistry, we show that small molecule transmitters, amines and peptides, are among the hormones present in these tissues, with many differentially distributed between the two sites (e.g., serotonin in the PO but not the SG). With several mass spectrometric (MS) methods, we identified many of the peptides responsible for the immunolabeling and surveyed the SG and PO for peptides for which no antibodies exist. By using MS, we characterized 39 known peptides [e.g., β‐pigment‐dispersing hormone (β‐PDH), crustacean cardioactive peptide, and red pigment‐concentrating hormone] and de novo sequenced 23 novel ones (e.g., a new β‐PDH isoform and the first B‐type allatostatins identified from a non‐insect species). Collectively, our results show that diverse and unique complements of hormones, including many previously unknown peptides, are present in the SG and PO of C. productus. Moreover, our study sets the stage for future biochemical and physiological studies of these molecules and ultimately the elucidation of the role(s) they play in hormonal control in C. productus. J. Comp. Neurol. 493:607–626, 2005. © 2005 Wiley‐Liss, Inc.