bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative analysis revealed a correlation of PIAS family genes expression with prognosis, immunomodulation and chemotherapy
Authors: Zhang Qiqi , Zhang Junkui , Lan Tianyi , He Jiayue , Lei Bin , Wang Hongnan , Mei Zhiqiang , Lv Chaoxiang 
Published: March 2024

Abstract
Background
Protein inhibitor of activated STATs (PIAS) has pleiotropic biological effects, such as protein post-translational modification, transcriptional coregulation and gene editing. It is reported that PIAS family genes are also correlated with immune cells infiltration in cancers that highlights their unnoticed biological role in tumor progression. However, the relationship of their expression with prognosis, immune cell infiltration, tumor microenvironment, and immunotherapy in pan-cancer has been rarely reported.

Methods
The multi-omics data were used to investigate the expression level of PIAS family members in pan-cancer, and the prognostic value of their expression in different tumors was analyzed by univariate Cox regression and Kaplan–Meier. Correlation analysis was used to investigate the relationship of PIAS gene expression with tumor microenvironment, immune infiltrating subtypes, stemness score and drug sensitivity. In addition, we also used wound healing and transwell assays to verify the biological effects of PIAS family gene expression on invasion and metastasis of HCC cells.

Results
We found that PIAS family genes expression is significantly heterogeneous in tumors by multi-genomic analysis, and associated with poor prognosis in patients with multiple types of cancer. Furthermore, we also found that genetic alterations of PIAS family genes were not only common in different types of human tumors, but were also significantly associated with disease-free survival (DFS) across pan-cancer. Single-cell analysis revealed that PIAS family genes were mainly distributed in monocytes/macrophages. Additionally, we also found that their expression was associated with tumor microenvironment (including stromal cells and immune cells) and stemness score (DNAss and RNAss). Drug sensitivity analysis showed that PIAS family genes were able to predict the response to chemotherapy and immunotherapy. PIAS family genes expression is closely related to tumor metastasis, especially PIAS3. High PIAS3 expression significantly promotes the migration and invasion of liver cancer cell lines (HCC-LM3 and MHCC97-H).

Conclusions
Taking together, these findings contribute to determine whether the PIAS family genes are a potential oncogenic target gene, which have important contribution for the development of cancer immunotherapy.
Will coronal alignment postoperatively be deteriorating in adult spinal deformity after long-fusion surgery?
Authors: Zhang Zifang , Wang Tianhao , Li Nianhu , Zheng Guoquan , Meng Chunyang 
Published: March 2024

Abstract
Background
To investigate whether the coronal alignment (CA) will deteriorate, and identify the risk factors for coronal malalignment (CM) developing in adult spinal deformity (ASD) after long-fusion surgery.

Methods
A multi-center retrospective study was performed, which included a total of 161 ASD patients who had undergone the surgical procedure of long-fusion (≥ 5 vertebras) with instrumentations in three medical centers. All of the participants were retrospectively reviewed, and subsequently assigned into the consistency group (C7 plumb line (C7PL) shifting towards the convex side of the main curve), and the opposition group (C7PL shifting towards the concave side). CM was considered if the coronal balance distance (CBD) being over 30 mm. A Kaplan–Meier curve and log-rank test were used to analyze the differences in CM-free survival during follow-up. Multivariate analysis via a Cox proportional hazards test was used to analyze the risk factors.

Results
Patients showing CM equaled 35 (21.7%) at the pre-operation, and that increased significantly up to 51 (31.7%) at the final follow-up (P = 0.04). In the consistency group, the incidence of CM at the final follow-up was much higher than that preoperatively (35:16, P = 0.002). CM-free survival time decreased significantly in patients with larger CBD correction, pelvic fixation and more instrumented segments, respectively, during follow-up (P < 0.05, log-rank test). Age ≥ 60 years, the consistency CA, pelvic fixation, CBD-correction ≥ 30 mm and fixed-vertebra ≥ 8 were risk factors for CM happening after surgery using multivariate regression analysis (P < 0.05).

Conclusions
The coronal alignments in ASD patients underwent long-fusion surgeries may deteriorate during follow-up, for which the risk factors include the consistency CA, age ≥ 60, fixed-vertebra ≥ 8, CBD-correction ≥ 30 mm and pelvic fixation.
Clinical spectrum of Transthyretin amyloidogenic mutations among diverse population origins
Authors: De Lillo Antonella , Pathak Gita A. , Low Aislinn , De Angelis Flavio , Abou Alaiwi Sarah , Miller Edward J. , Fuciarelli Maria , Polimanti Renato 
Published: March 2024

Abstract
Purpose
Coding mutations in the Transthyretin (TTR) gene cause a hereditary form of amyloidosis characterized by a complex genotype-phenotype correlation with limited information regarding differences among worldwide populations.

Methods
We compared 676 diverse individuals carrying TTR amyloidogenic mutations (rs138065384, Phe44Leu; rs730881165, Ala81Thr; rs121918074, His90Asn; rs76992529, Val122Ile) to 12,430 non-carriers matched by age, sex, and genetically-inferred ancestry to assess their clinical presentations across 1,693 outcomes derived from electronic health records in UK biobank.

Results
In individuals of African descent (AFR), Val122Ile mutation was linked to multiple outcomes related to the circulatory system (fold-enrichment = 2.96, p = 0.002) with the strongest associations being cardiac congenital anomalies (phecode 747.1, p = 0.003), endocarditis (phecode 420.3, p = 0.006), and cardiomyopathy (phecode 425, p = 0.007). In individuals of Central-South Asian descent (CSA), His90Asn mutation was associated with dermatologic outcomes (fold-enrichment = 28, p = 0.001). The same TTR mutation was linked to neoplasms in European-descent individuals (EUR, fold-enrichment = 3.09, p = 0.003). In EUR, Ala81Thr showed multiple associations with respiratory outcomes related (fold-enrichment = 3.61, p = 0.002), but the strongest association was with atrioventricular block (phecode 426.2, p = 2.81 × 10− 4). Additionally, the same mutation in East Asians (EAS) showed associations with endocrine-metabolic traits (fold-enrichment = 4.47, p = 0.003). In the cross-ancestry meta-analysis, Val122Ile mutation was associated with peripheral nerve disorders (phecode 351, p = 0.004) in addition to cardiac congenital anomalies (fold-enrichment = 6.94, p = 0.003).

Conclusions
Overall, these findings highlight that TTR amyloidogenic mutations present ancestry-specific and ancestry-convergent associations related to a range of health domains. This supports the need to increase awareness regarding the range of outcomes associated with TTR mutations across worldwide populations to reduce misdiagnosis and delayed diagnosis of TTR-related amyloidosis.
Mimesis and Mythos in Aristotelian Arabic Poetics
Authors: Harb Lara 
Published: March 2024

AbstractThe Aristotelian sense of mimesis (i.e., fictional representation of an evoked world through plot and characters) continues to shape modern views of literature. The medieval Arabic reception of the concept of mimesis and its closely related concept of mythos (fable, story, or plot) through Aristotle’s Poetics reveals a different conception of literature—one that this article argues is not representational but analogical. While Aristotle sees mythos/plot as forming the main vehicle through which mimesis manifests itself in poetry, mythos (khurāfa) according to the philosophers of the Islamic world was irrelevant for the poetic. This is because they understood mythos not as “plot” but as referring, on the one hand, to the fictionality and fantasticness of poetic metaphor, which they deemed inessential for the success of poetry; on the other hand, they understood mythos as “story,” in which case they relegated to it a rhetorical function of persuasion rather than a poetic function of make-believe. Fundamentally, in both the poetic and the rhetorical, mimesis was understood as the expression of the comparable, not the representation of the likeness of an evoked world. This exposes an Arabic conception of “mimesis” that reflects a literature that seeks comparisons and demands the inference of similarities from the reader.
Lost Daughters and Sunken Ships
Authors: Young Hershini Bhana 
Published: March 2024

Abstract
This essay develops the idea of salvage as a material practice around ships’ cargo and as an archival epistemology. Using a legal court case concerning 43 million dollars worth of silver bars salvaged from the wreck of the SS Tilawa, a website constructed by the son of one of the survivors of the wreck, and the Facebook story of drowned South African cadet Akhona Marara Felicity Geveza, the essay centers the nonevent that unfolded in the wake of the supposed catastrophe. It focuses on the role of economic extraction in creating the historically entangled Indian and African diasporas. The piece insists on salvage as a material practice, as a methodology, and as a mode of temporality that oscillates between the cacophony of a present and the silence of shadowy depths. The threading throughout the essay of the autobiographical, mainly through readings of the author's Indian father's unpublished memoirs as he journeyed from India to Pretoria, stresses the role of misremembering and the tangibility of an archive contained in pillowcases and written in fading ink.
Epiphytic Lives
Authors: Chea Jolie 
Published: March 2024

AbstractThis essay troubles the application of Marianne Hirsch's concept of “postmemory” to the Cambodian postwar generation's relationship to the Khmer Rouge regime and the war, instead proposing the term “nonmemory” as a description of what we who are not survivors of that war tend to understand as an absence of narrative regarding the war and the regime. In doing so, it calls attention not to the silence of Cambodian survivors but to Cambodian American second-generation treatments of survivor silence. Instead of “breaking the silence,” it offers for the purposes of deep and critical contemplation the figure of the kapok tree, reputable and fabled to Cambodians, toward a concept of the epiphytic, understood as a multilayered life-giving, anti- and de-colonial force in nature.
Beyond Double Identity, beyond Periphery: Chinese Ethnic Korean Poetry as Borderland Literature
Authors: Xie Miya Qiong 
Published: March 2024

Abstract
In East Asia, Chinese ethnic Korean literature has drawn scholarly attention from Chinese, South Korean, and Japanese scholars. They mostly situate the literature within a “double identity” (ijung chŏngch’esŏng 이중정체성) that is both Korean and Chinese and examine how the literature sheds light on the relations and tensions between the two sides of the identity. This article explores the critical potential of the literature beyond the limits of a double identity and within a borderland milieu. The Yanbian Ethnic Korean Autonomous Prefecture is located along the northeast border of China and is adjacent to the Korean Peninsula, Russia, and Japan. Through a close reading of the early postwar poetry produced in the region by the representative Chinese ethnic Korean poet Ri Uk, the article examines how Chinese ethnic Korean literature may serve as a nexus of connection and negotiation among multiple cultures. Those who are familiar with the region know that these cultures have been deeply alienated from each other during multiple rounds of imperialization by regional and global powers. A close examination of the multilateral connection of a borderland ethnic literature with regional power holders offers insights into the ways cultures connect despite historical conflicts and ideological opposition.
The antibacterial efficiency of Chitosan photodynamically activated Phycocyanin, and Morinda Oleifera against S.mutans and the bonding strength between composite resin and caries-affected dentin
Authors: Alanzi Amer , Ali Khan Azmat , Amer Mahmood  , Mohammad Amjad Kamal  , Eisha Abrar Baig  
Published: March 2024

Objective: Evaluation of contemporary disinfection techniques, Moringa Oleifera (M.Oleifera), Phycocyanin activated by photodynamic therapy (PDT), and Chitosan, on S.mutans survival rate and bond integrity of composite to carious-affected dentin (CAD).
Methods: The in vitro study was conducted at King Saud University and concluded within three months. Sixty mandibular teeth with cavities extending to the middle third of the dentin were sterilized. S.mutans was inoculated onto the CAD surface of twenty samples. The samples were randomly divided into four groups (n: 15) based on various disinfection regimes. Group-1 received 2% CHX, Group-2 Phycocyanin activated by photodynamic therapy (PDT), Group-3 Chitosan, and Group-4 M.oleifera. S.mutans survival rate was calculated. Ten CAD samples from each group were restored using composite. The bond integrity of samples was assessed using a Universal testing machine (UTM) and failure mode using a stereomicroscope. Analysis of variance (ANOVA) and Tukey’s Post Hoc test were used to calculate statistical significance (p=0.05).
Results: Group-2 samples subjected to Phycocyanin activated using PDT, displayed minimal survival rate (0.24 ± 0.05 CFU/ml) of S.mutans.Group-1 samples treated with CHX exhibited the highest count of S.mutans (0.69 ± 0.12 CFU/ml). The most robust bond was observed in Group-3 (Chitosan) samples (19.33 ± 0.47 MPa). In contrast, SBS values were lowest in Group-1 (CHX) treated study samples (13.17 ± 1.88 MPa).
Conclusion: Chitosan, Phycocyanin activated by PDT, and Moringa Oleifera extract exhibit potential as viable substitutes for chlorhexidine (CHX) in clinical settings, presenting the possibility of better eradication of S.mutans and greater adhesive strength to CAD.
doi: https://doi.org/10.12669/pjms.40.5.8800
How to cite this: Alanazi AM, Khan AA, Mahmood A, Kamal MA, Baig EA. The antibacterial efficiency of Chitosan photodynamically activated Phycocyanin, and Morinda Oleifera against S.mutans and the bonding strength between composite resin and caries-affected dentin. Pak J Med Sci. 2024;40(5):---------. doi: https://doi.org/10.12669/pjms.40.5.8800
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
SOME ENVIRONMENTAL ASPECTS OF MONITORING OF TRICHINELLOSIS IN THE KIROV REGION AND THE KOMI REPUBLIC
Authors: Zhdanova Olga B. , Okulova Iraida I. , Chasоvkikh Olga V. 
Published: March 2024

Background. Гельминт Trichinellа относится к классу Nematoda (Rudolphi, 1808); к отряду Trichocephalida (Skrjabin et Schulz, 1928); вид Trichinella spiralis (spiralis) (Owen, 1835) имеются также различные вариететы. В данном исследовании изучен вариетет Trichinellа spiralisIn order to prevent trichinosis in the population and animals, methods of diagnosis of trichinosis are widely used.
Purpose. The aim of this work was to study the distribution of trichinosis among wild animals of the Kirov region and the Komi Republic and to analyze the localization of Trichinella sp. in the muscles of animals and the features of their capsules in various animal species by the methods of trichinelloscopy and the digestion of muscle tissue in artificial gastric juice.
Materials and methods. The capsule index was calculated using morphometric analysis. The most sensitive among the methods of postmortal diagnostics is the method of digestion of muscle tissue in artificial gastric juice, therefore it is used as a method for clarifying the diagnosis in doubtful cases (sarcocystosis, alariosis).
Results. The article provides data on the distribution of trichinosis in wild animals of the Kirov region and the Komi Republic. Information on the selective distribution of Trichinella larvae in wild animals, the morphological features of the capsules and the intensity of invasion in various animals are presented.
Conclusion. Thus, it has been established that the distribution of Trichinella larvae in muscle depends on the species of animals and parasites.
EVALUATION OF THE RESISTANCE OF STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI REGARDING PLANT EXTRACTS ON MODELS OF PLANKTONIC CELLS AND BIOFILM
Authors: Khabaeva Zinaida G. , Agaeva Fatima A. , Marzoeva Diana A. , Burnatseva Alina A. , Butkhudze Veriko D. 
Published: March 2024

The resistance of Staphylococcus aureus and Escherichia coli was assessed using two ecological models: planktonic cells and biofilms. As herbal preparations, 8 official medicinal plants with pronounced antibacterial and anti-inflammatory properties, purchased in the pharmacy network, produced by ZAO Krasnogorskleksredstva and collected in North Ossetia-Alania, were used. Antibiofilm activity was determined in common garlic (Allium sativum) and common thyme (Thymus vulgaris). Used pomace from the green mass of plants, alcohol tinctures.
The severity of the antibacterial activity of the tested preparations was determined by the standardized Kerby-Bauer method. To create an artificial biofilm, the method of isolated biofilm and the method of culturing static biofilms in liquid were used; to assess the relative growth rates, staining with gen-cyan violet was carried out.
The conducted studies (pomace from the green mass of plants) in relation to the culture of Staphylococcus aureus revealed either the absence of a zone of growth inhibition (Pinus sylvestris, Thuja occidentalis) or the presence of a low sensitivity of planktonic cells to the tested preparations. When using the dry mass of pharmaceutical preparations (alcohol tinctures), antibacterial activity against planktonic Staphylococcus aureus cells was detected in extracts of garlic and common thyme (high), coltsfoot and eucalyptus rod (sufficient). Escherichia coli strains were resistant to almost all tested drugs (no growth retardation or low sensitivity). Antibiofilm activity was manifested only at the growth stage (stable adhesion) when using extracts of garlic and common thyme in relation to Staphylococcus aureus and Escherichia coli.
COMPREHENSIVE ASSESSMENT OF THE QUALITY AND BIOLOGICAL VALUE OF APPLE FRUIT OF DIFFERENT VARIETIES GROWN IN THE CONDITIONS OF THE REPUBLIC OF ADYGEA
Authors: Tsikunib Aminet D. , Demchenko Yulia A. , Ezlyu Fatima N. , Pavliuchenko Svetlana A. , Osmani Sumeya A. 
Published: March 2024

Background. Apple fruits are a valuable food product with a unique chemical composition that determines the taste and biological value. The chemical composition of fruits depends on the variety of apples, ripening terms, conditions and place of growth, which makes research relevant in the conditions of a certain region, including the Republic of Adygea, where horticulture not only has deep historical roots, but has been actively reviving and developing in recent decades. with the use of new technologies and the introduction of new varieties.
Target. Carrying out a comprehensive assessment of the quality and biological value of apple fruits of different varieties grown in the conditions of the Republic of Adygea on the basis of a study of organoleptic, physicochemical and biochemical indicators.
Materials and methods. The objects of the study were samples of apple fruits of the agricultural enterprise LLC “Yumiks” of 5 varieties: Braeburn, Granny Smith, Idared, Florina, Simirenko, grown in the conditions of the Republic of Adygea. In the samples under study, the determination of organoleptic, physicochemical and biochemical parameters was carried out: soluble solids (SS), titratable acids per malic acid, vitamin C, total sugars, iron, polyphenol oxidase activity, antioxidant activity (AOA). Statistical data processing was carried out in MS Excel 2016.
Results. For the first time, on the basis of a complex of organoleptic, physicochemical and biochemical methods, studies of apple varieties grown in the Republic of Adygea were carried out: Florina, Idared, Granny Smith, Braeburn, Simirenko. It has been established that the highest index of sensory quality has apple varieties Braeburn and Simirenko, the Braeburn variety is distinguished by a high content of solids and sugars, more harmonious taste, i.e. the optimal ratio of sugar to acid is found in the Braeburn and Florina varieties. The highest content of vitamin C and bioavailable iron is characteristic of Braeburn and Simirenko apple varieties. Polyphenol oxidase activity in Simirenko, Idared and Florina varieties was higher than in samples of other varieties, while a higher AOA value was found in Granny Smith.
Conclusion. The data obtained by us on the basis of a comprehensive study of apple varieties new to the Republic of Adygea, but already in a certain consumer demand, will allow us to give an objective assessment of their contribution to the biological value of the diets of various population groups, and also, subject to further monitoring studies, can be used gardeners of the Republic of Adygea when expanding the area under varieties with the highest taste and stable indicators of biological value.
DEVELOPMENT OF A MODIFIER FOR GIVING MATERIALS FROM EFFICIENTLY PROCESSED WOOD BIOMASS SPECIAL PROPERTIES
Authors: Zyryanov Mikhail A. , Medvedev Sergey O. , Kukushkin Alexey A. , Kuchina Kseniya Yu. , Kositsyna Anna S. , Root Evgeny V. 
Published: March 2024

Background. Wood is a unique material in its structure. However, materials made from wood raw materials have such negative properties as insufficient bio- and fire-resistance. In turn, when organizing and improving the efficiency of logging activity, large amounts of practically unused cutting residues is produced. Their modification for the purposes of the forest industry is an effective tool for creating many valuable and demanded products. In particular, arilazo-β-dicarbonyl compounds are widely used as syntons for the production of heterocyclic compounds. Among heterocycles, a large number of compounds have found application in the form of biologically active substances that have been successfully and for a long time used as pesticides for the wood processing industry.
Purpose. Synthesis of p-nitrophenyl hydrobutanons, cyclocondensation to form pyrazoles and study of their chemical properties of the substances first obtained. Proof of structure by modern spectral analysis methods.
Materials and Methods. Research methods include: organic synthesis; UV spectrometry, 1H NMR, 13H NMR.
Results. Four new compounds were synthesized: 4-methoxy-1-(p-chloro(bromo)phenyl)-2-(p-nitrophenylhydrazo)-1,2,3-butantrions and 3(5)-methoxy-5(3)-(p-chloro(bromo)phenyl)-4-(p-nitrophenylhydrazo)-1H-pyrazoles. Amines were prepared by the reduction of the related nitrosopyrazoles. The acylation reaction was demonstrated for the obtained amine. The structures of all synthesized compounds were proved by modern methods of analysis.
Conclusion. Thus, we synthesized p-nitrophenylhydrazobutanetriones with a chloro(bromo)phenyl substituent, their cyclization products with hydrazine, and N-(5-(4-chloro(bromo)phenyl)-3-(methoxymethyl)-1H-pyrazole-4-yl)acetamides based on them. The structure of the obtained substances was determined by spectral methods of analysis.