bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis
Authors: Ulloque-Badaracco Juan R. , Alarcon-Braga Esteban A. , Hernandez-Bustamante Enrique A. , Von-Koeller-Jones Beatrix M. , Huayta-Cortez Miguel , Saavedra-Custodio Esduardo , Herrera-Añazco Percy , Benites-Zapata Vicente A. 
Published: February 2025

Background and aimsChildhood and adolescent obesity is a global public health concern. Obesity induces several metabolic disturbances. Several studies have explored the association of vitamin B12, folate, and homocysteine (Hcy) with obesity. This study aimed to synthesize the available evidence regarding the differences in serum levels of vitamin B12, Hcy, and folate among children or adolescents with and without obesity.MethodsA random-effects meta-analysis using the Sidik-Jonkman method and corrected 95% confidence interval (CI) using the truncated Knapp-Hartung standard errors was used for all meta-analyses. Standardized mean difference (SMD) with the corresponding 95% CI was used as the only effect size. The Cochran’s Q test and the I2 statistic were used to evaluate between-study heterogeneity. Publication bias was assessed using funnel plots and the Egger test.ResultsTwenty studies were included with a combined study population of 7,791 patients. There were no significant differences between children/adolescents with and without obesity with respect to serum vitamin B12 levels (SMD: −0.24; 95% CI: −0.53 to 0.06; p &gt; 0.05, I2 = 74.93%) and folate levels (SMD: −0.12; 95% CI: −0.29 to 0.06; p &gt; 0.05, I2 = 19.6%). However, children/adolescents with obesity had significantly higher Hcy levels compared to counterparts without obesity (SMD: 0.77; 95% CI: 0.39 to 1.14; p &lt; 0.001, I2 = 86.4%).ConclusionChildren and adolescents with obesity had higher Hcy levels than those without obesity. However, no significant differences were found for vitamin B12 and folate levels. Hcy may play a role in the development of obesity in this population.
Demystifying death: a qualitative study using the behavior change wheel framework to explore the palliative care education experiences of doctors, nurses, and community residents
Authors: Yang Guodong , Wang Renxiu , Zhao Jun , Ding Kaiwen , Xu Longhui , Liu Yue , Han Xiaoxuan , Zhang Chao , Xu Cuiping 
Published: February 2025

ObjectiveTo explore the palliative care education experiences of doctors, nurses, and community residents.MethodsA semi-structured interview was conducted with 2 doctors, 8 nurses and 9 community residents in Jinan. Content analysis and behavior change wheel theory were used to analyze the interview content.ResultsMotivation: The road to be taken; Emotional touch of personal experiences; Prepare early; Not now. Capability: Multiple cognition; Need for a topic catalyst; Trust bias; Disconnect between learning and application; Treading on thin ice. Opportunity: Willing but unable; The Need for a larger voice and greater participation.ConclusionCommunity palliative care education requires greater attention. Community residents exhibit diverse perceptions and attitudes toward palliative care, reflecting the influence of personal experiences and sociocultural factors. Innovating the content and format of educational resources and enhancing education for community residents and medical staff will facilitate palliative care.
Perceptions of anti-racism efforts and mental health among students in higher education in the United States
Authors: Oh Hans , Karcher Nicole R. , Besecker Megan , DeVylder Jordan E. , Lincoln Karen D. 
Published: February 2025

BackgroundAnti-racism efforts are imperative for campus communities, yet little is known about whether perceiving their presence on campuses relates to a range of mental health outcomes among students.MethodsWe analyzed data from the Healthy Minds Study 2020–2021 (N = 110,203). Using multivariable logistic regression, we examined the associations between perceptions of anti-racism efforts and several mental health outcomes.ResultsIndividuals who disagreed that their schools combatted racism in their campus communities had significantly greater odds of mental health problems (depression, anxiety, psychotic experiences, suicidal ideation, suicide plan, suicide attempt, perceived need for help, and loneliness), and lower odds of flourishing when compared with those who strongly agreed with the statement. For most outcomes, we observed an apparent dose–response association.ConclusionPerceiving the presence of anti-racism efforts on campuses was inversely associated with mental health problems, calling for more research to test the effects of anti-racism efforts on mental health.
Expression of CSTF2 in oral squamous cell carcinoma and its relationship with immune infiltration and poor prognosis
Authors: Aierken Zumulaiti , Muhetaer Muertiza , Lei Zhang , Abudourousuli Ainiwaerjiang 
Published: February 2025

BackgroundOral squamous cell carcinoma (OSCC) is a prevalent and devastating malignancy of the oral cavity that profoundly affects patient survival and quality of life (QOL). Cleavage Stimulation Factor Subunit 2 (CSTF2) is known to influence tumor development across multiple cancer types. However, its specific association with patient prognosis and immune cell infiltration in OSCC remains insufficiently understood.MethodsTo assess the expression levels and prognostic implications of CSTF2 in OSCC, comprehensive data were acquired from The Cancer Genome Atlas (TCGA) and subsequently normalized. Immunohistochemical staining of tissue microarrays was performed to analyze CSTF2 expression in the OSCC samples. Differences in CSTF2 expression between OSCC and adjacent non-cancerous samples were evaluated using the Wilcoxon rank-sum test. Functional enrichment analyses have been performed to identify biological pathways and functions associated with CSTF2. The relationship between the infiltration of various immune cells and CSTF2 expression levels was assessed using single-sample gene set enrichment analysis (ssGSEA). Ultimately, the prognostic significance of CSTF2 was evaluated through Kaplan–Meier survival analysis, in conjunction with univariate and multivariate Cox regression analyses, as well as receiver operating characteristic (ROC) curves.ResultsHigh CSTF2 expression was observed in OSCC and associated with unfavorable clinicopathological variables, including histological grade and lymphnode neck dissection. Functional enrichment analysis indicated that CSTF2 plays a role in epidermal development and differentiation, immunoglobulin complexes, peptidases and endopeptidase inhibitor activity, and cytochrome P450 metabolic processes. Additionally, the overexpression of CSTF2 exhibited a negative correlation with the infiltration of immature dendritic cells (iDCs), cytotoxic cells, and plasmacytoid dendritic cells (pDCs). Notably, elevated CSTF2 expression is significantly associated with reduced patient outcomes.ConclusionElevated CSTF2 expression in OSCC is associated with poor prognostic outcomes, highlighting its capacity to function as an innovative prognostic biomarker and a target for therapeutic interventions.
Assessment of trade-off balance of maize stover use for bioenergy and soil erosion mitigation in Western Kenya
Authors: Jindo Keiji , Ghaffari Golaleh , Lamichhane Manisha , Lazarus Asher , Sawada Yoshito , Langeveld Hans 
Published: February 2025

IntroductionKakamega Forest, Kenya's last tropical rainforest, faces threats from escalating demands for firewood, charcoal, and agricultural expansion driven by population growth. Sustainable resource management is critical, with maize stover—a common lignocellulosic crop residue—proposed as a source for biogas and bioslurry production. However, its removal raises concerns about soil fertility and erosion control. This study evaluates the trade-off between maize stover's use for energy generation and its role in soil erosion mitigation in Western Kenya, considering biophysical conditions and households' socio-economic status.MethodsSocio-economic data were collected from 91 households to assess energy usage. Soil erosion was quantified using the Revised Universal Soil Loss Equation (RUSLE). High-resolution remote sensing, self-organizing maps (SOM), and Hidden Markov Models (HMM) were integrated for annual field condition monitoring. A decision-tree machine learning model identified farm characteristics favorable for maize stover use in biogas production.ResultsLarger households were found to consume more energy per capita, while proximity to forests did not significantly influence firewood or charcoal consumption. Maize yields were significantly associated with land preparation methods (tractor, oxcart, or manual plowing; p &lt; 0.001) and field size (p &lt; 0.05). Remote-sensing data indicated that the distance between homesteads and fields impacted crop growth status. RUSLE analysis revealed that soil erosion was more strongly influenced by landscape features than by soil properties or farming practices.DiscussionThe integration of SOM-HMM and microsatellite data improved field monitoring and data accuracy, providing valuable insights for sustainable agricultural practices in Kakamega. These findings highlight the complex trade-offs between maize stover utilization for energy production and its role in maintaining soil health, emphasizing the need for balanced resource management strategies.
In Vitro and In Vivo Evaluation of Bacillus Strains as Prophylactic Agents Against Porcine Epidemic Diarrhea Virus
Authors: Chen You-Jia , Tsai Chia-Fang , Hsu Chin-Wei , Chang Hui-Wen , Liu Je-Ruei 
Published: February 2025

Porcine epidemic diarrhea virus (PEDV), particularly the highly virulent G2b strains, has inflicted substantial economic losses on the global swine industry. This study evaluated the prophylactic effects of three Bacillus strains—B. amyloliquefaciens LN, B. licheniformis CK, and B. velezensis AC—against PEDV infection using in vitro and in vivo models. In vitro experiments with Vero cells demonstrated that B. amyloliquefaciens LN increased cell viability, reduced PEDV-N expression, and modulated proinflammatory cytokine responses. In vivo, piglets supplemented with B. amyloliquefaciens LN exhibited alleviated diarrhea symptoms, suppression of fecal viral RNA shedding to below the detection limit, and restoration of gut microbiota balance by increasing Bacteroidetes and reducing Proteobacteria abundance. Mechanistic studies indicated that the measured interferon (IFN)-related genes were not significantly influenced in this study, suggesting that the protective effects of B. amyloliquefaciens LN may involve the modulation of inflammatory responses and the inhibition of viral replication through reduced PEDV-N expression. This study illustrates the potential of using B. amyloliquefaciens LN as a feed additive to prevent PEDV infection.
Lesões de pele no intraoperatório de cirurgias robóticas: série de casos
Authors: Olle Fernanda Borcelli , Trevilato Denilse Damasceno , Artioli Renata Pereira Silva , Fagundes Maria Anobes Bonet Grespan , Neves Charel de Matos , Paula Saul Ferraz de , Pedroso Martina Madalena , Montanari Carolina Caruccio 
Published: February 2025

Introdução: A realização de cirurgias robóticas tem trazido avanços em diversos aspectos, incluindo precisão e rápida recuperação. No entanto, junto com os benefícios, surgem preocupações sobre potenciais complicações durante o procedimento, incluindo lesões de pele. Objetivo: Relatar cinco casos de pacientes que apresentaram lesão de pele no intraoperatório de cirurgia robótica. Métodos: Trata-se de um estudo observacional, retrospectivo, descritivo de uma série de cinco casos. Foi conduzido em um Centro Cirúrgico de um hospital privado de Porto Alegre, Rio Grande do Sul, Brasil. Os dados foram coletados em prontuários de pacientes que realizaram procedimentos entre janeiro e dezembro de 2022. Estudo aprovado pelo comitê de ética e pesquisa sob número CAAE: 73725323.7.0000.5330. Resultados: Dos 556 pacientes que realizaram procedimento cirúrgico por técnica robótica em 2022, cinco (0,9%) apresentaram lesão de pele documentada em prontuário no pós-operatório. Quatro eram do sexo masculino, a maioria com idade superior a 60 anos e comorbidade de hipertensão arterial. Quatro foram classificados com maior risco de desenvolvimento de lesões na ELPO, dois apresentaram lesões de pele por dispositivo médico e três por posicionamento intraoperatório. Conclusão: Este estudo analisa cinco casos de lesões de pele durante cirurgias robóticas, enfatizando a importância da prevenção com a ELPO. Apesar do menor tempo cirúrgico em cirurgias robóticas, lesões de pele persistem em pacientes de alto risco, ressaltando a importância da utilização da ELPO para medidas preventivas mais eficazes. Mais pesquisas são cruciais para desenvolver estratégias inovadoras e melhorar a segurança dos pacientes.
A utilização do treinamento de força na prevenção de quedas em idosos
Authors: Batista Marco Antonio Leitão , Santos Ana Carine dos , Machado Rafael Bacelar , Alves Sandra Gabriela Ribeiro , Alves Even Herlany Pereira 
Published: February 2025

A síndrome do idoso frágil se origina a partir do declínio das funções fisiológicas dos idosos. Observa-se entre estes pacientes a diminuição de atividades físicas, perda de peso, queda da velocidade de mobilidade e redução das funções cognitivas, sendo estas situações avaliadas a partir dos critérios de fragilidade de Fried, especialmente em idosos com doenças crônicas. Nesse sentido, este estudo teve como objetivo evidenciar estudos que verificaram sobre a utilização do treinamento de força na prevenção de quedas em idosos e sua influência na qualidade de vida de idosos com fragilidade. Identificou-se que a sarcopenia aumenta com a idade, sendo mais comum em mulheres, idosos institucionalizados e idosos hospitalizados. Sendo assim, identificou-se que a prática de exercícios físicos, é capaz de impedir que idosos se tornem frágeis. Com relação à queda, ela é a variável clínica mais presente, sendo um histórico comum em boa parte deles. Os maus-tratos foram objeto de poucos estudos, porém conclui-se que idosos homens e depressivos são as maiores vítimas de violência física e verbal. O envelhecimento natural traz consigo diversas alterações fisiológicas, como a perda de massa muscular e a diminuição da força, que podem comprometer a autonomia e a independência dos indivíduos. Essas mudanças são conhecidas como sarcopenia e estão associadas a um maior risco de quedas, fraturas e outras complicações. O treinamento de força surge como uma estratégia eficaz para reverter esses efeitos do envelhecimento fortalecendo os músculos, melhorando o equilíbrio, coordenação, prevenindo quedas, mantendo a autonomia e a qualidade de vida dos idosos.
Neural activity analysis of depressive disorder model mice using the Toyohashi probe
Authors: Muramatsu Joichiro , Sonoda Kazuhiro , Yamashita Koji , Sasaki Hinata , KAWANO Takeshi , Numano Rika 
Published: February 2025

Abstract
Major depressive disorder (MDD) is one of the most chronic disease and life-threatening &#xD;illnesses globally. However, mainstay medications do not provide sufficient relief for &#xD;depressive patients with MDD. Thus, there is an urgent need for the development of analysis &#xD;methods that allow drug screening and observation of phenotypes. We developed a tiny &#xD;microneedle electrode block module called the Toyohashi probe, which can be inserted into &#xD;a mouse brain with minimal invasiveness using the vapor–liquid–solid method. Photo&#xD;response was measured in the primary visual cortex of Nrf2 knockout (KO) mice using the &#xD;Toyohashi probe. We then successfully identified characteristic electrophysiological &#xD;phenotypes, such as abnormal excitability of neurons and higher firing frequency and spikes, &#xD;in Nrf2 KO mice. In conclusion, our new microneedle electrode can be used in vulnerable &#xD;disease model mice to record stable neuronal activity stably in vulnerable disease model &#xD;mice and observe characteristic phenotypes as indicators for drug efficacy.
HUBUNGAN PENGETAHUAN PEKERJA HARIAN LEPAS SEKTOR KONSTRUKSI WILAYAH KABUPATEN TEGAL TERHADAP KEPEMINATAN PESERTA ASURANSI BPJS KETENAGAKERJAAN
Authors: Rosmalia Rosmalia , Sukmandari Erna Agustin , Atmoko Dwi 
Published: February 2025

Sektor konstruksi memiliki aktifitas pekerjaan kompleks serta penggunaan tenaga kerja secara padat karya sehingga kemungkinan risiko bahaya yang berdampak cidera sampai meninggal dapat terjadi pada pekerjanya. Aspek penting dalam pemenuhan kompetensi tenaga kerja sektor konstruksi salah satunya pengetahuan tentang keselamatan dan kesehatan kerja. Pekerja Harian Lepas (PHL) sebagai salah satu tenaga yang sering dijumpai pada proyek-proyek konstruksi, lemah dalam memperoleh perlindungan keselamatan dan kesehatan kerja sebab tidak ada perjanjian kerja secara tertulis dari pihak pemberi kerja. Perekrutannya berdasarkan kebutuhan dan terbatas waktu sehingga bila mengalami kecelakaan kerja, tidak ada jaminan pembiayaan pengobatan atau biaya kematian yang wajar. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan PHL mengenai keselamatan kerja dan jaminan keselamatan kerja terhadap kepeminatan sebagai peserta asuransi jaminan keselamatan kerja yaitu BPJS Ketenagakerjaan. Metode penelitian berupa studi cross sectional dengan analisis deskriptif korelatif. Instrumen penelitian berupa kuesioner meliputi 4 aspek yaitu : tanggung jawab, keselamatan berbasis perilaku, alat pelindung diri, dan produk BPJS Ketenagakerjaan. Jumlah responden 112 pekerja proyek konstruksi di wilayah Kabupaten Tegal yang sedang berlangsung. Analisis data menggunakan aplikasi IBM SPSS 23 dengan uji statistik chi square. Hasilnya adalah karakteristik pekerja berdasarkan usia 97,3% merupakan usia produktif dan pendidikan 35,7% tamatan SD disusul 32,1% tamatan SMP. Pekerja sebanyak 22,6% tidak berminat menjadi peserta asuransi beranggapan jika ada kecelakaan kerja adalah tanggung jawab mandor atau pemberi usaha. Perilaku aman sekedar himbauan dari pemberi kerja/mandor sehingga perilaku rasa aman pekerja diciptakan berdasarkan pengalaman kerja masing-masing. Tidak tersedia APD pendukung bekerja di ketinggian, belum terpapar secara rinci tata cara mengikuti produk asuransi BPJS Ketenagakerjaan, serta rasa khawatir akan jumlah iuran yang nanti menjadi beban setiap bulan. Terdapat hubungan pengetahuan PHL tentang keselamatan kerja dengan peminatan peserta asuransi jaminan keselamatan kerja atau BPJS Ketenagakerjaan, dengan nilai p=0,009 < α dan nilai RR=0,653. Pekerja yang berpengetahuan K3 baik berpeluang 0,653 kali dalam kepeminatan peserta BPJS Ketenagakerjaan dibandingkan peserta yang kurang berpengetahuan K3.
Dampak dan Monitoring pada Pekerja Terpapar Benzena
Authors: Winata Susanty Dewi 
Published: February 2025

AbstrakBenzena merupakan salah satu senyawa hidrokarbon aromatik yang banyak digunakan di sektor industri karet, penyulingan minyak, pabrik kimia, pabrik sepatu, dan industri yang terkait dengan minyak. Benzena juga ditemukan pada fasilitas publik seperti asap rokok, pompabensin, pembakaran bahan bakar mobil, dan sebagainya. American Conference of Government Industrial Hygienists (ACGIH) menggolongkan benzena kedalam bahan karsinogen group-1A. Pengukuran benzena dapat dilakukan dalam udara ekspirasi, urin dan darah.Pajanan benzena dalam kadar yang rendah di tempat kerja dapat dinilai dengan baik oleh t,t-asam mukonat dan S-asam fenil merkapturat dalam urin. Dengan nilai ambang batas (NAB) benzena 0,5 ppm, ACGIH merekomendasikan pengukuran t,t-asam mukonat dan asam fenilmerkapturat dalam urin dengan indeks monitoring biologis 0,5 mg/g kreatinin sedangkan untuk asam mukonat yaitu 25Âµg/g kreatinin. Diperlukan berbagai pengendalian pajanan agar para pekerja terlindung dari dampak buruk benzena terhadap kesehatan pekerja.Â Â AbstractBenzena is a aromatic hydrocarbon prevalently used in the rubber industry, oil refinery, chemical industry, shoe factories and other industries correlated with oil. Benzena can also be found in public facilities as it is contained in smoke of the cigarette, petrol station and the result of fuel burning. ACGIH (American Conference of Government Industrial Hygienists) classifies Benzena in Carcinogen group 1A. Benzena measurement can be conducted in the expiration air, urine and blood. The exposure of benzena at working place can be measured precisely by t-Mukonat Acid and S-Phenylmercapturat acid content in the urine with biological monitoring index of 0,5 mg/g Creatinin for Mukonat Acid and 25Âµg/g Creatinin for Phenylmercapturat acid. There should be control in the level of the chemicals to protect the workers from the harmful effects of benzena to their health.
Manfaat Meniran (Phyllanthus niruri) untuk Penyembuhan Demam Berdarah Dengue
Authors: Rammang Laotesa , Majawati Esther Sri 
Published: February 2025

Abstrak Saat ini insiden demam berdarah dengue cenderung meningkat dari tahun ke tahun. Penyebab meningkatnya jumlah kasus demam berdarah karena semakin padatnya penduduk, tingginya mobilitas penduduk, dan kurangnya kesadaran masyarakat tentang pentingnya lingkungan yang bersih. Artinya penyebaran nyamuk akan semakin luas, seiring dengan semakin bertambahnya penduduk. Demam berdarah dengue (DBD) disebabkan oleh virus dengue yang ada pada nyamuk Aedes aegypty penularnya. Tidak semua nyamuk dapat menyebabkan demam berdarah dengue, hanya nyamukÂ yang mengandung virus dengue yang dapat menyebabkan penyakit. Demam berdarah dengue menyebabkan seseorang mengalami peradangan hati, daya tahan tubuh menurun. Meniran (Phyllanthus niruri) sering digunakan sebagai pengobatan herbal pada demam berdarah dengue . Penelitian medis tentang meniran terus dilakukan, terutama efeknya yang dapat meningkatkan daya tahan tubuh dan antihepatoksik,yang pada penderita demam berdarah dengue sering terjadi penurunan daya tahan tubuh dan peradangan hati.Â Kata kunci : Meniran, DBD, falvanoid, filatin, hepofilatinAbstractCurrently the insidens of dengue fever tend to increase from year to year. The cause of the increasing number of cases of dengue fever due to of dense population,Â high population mobility andÂ public minimal awareness of the importance of a clean environment. The spreading of mosquitoes will be more extensive, along with the increasing population. Dengue fever is caused by dengue virus and Aedes aegypty as the vector. Â Not all mosquitoes causing dengue fever , the only mosquitoes carrying the dengue virus can cause disease. Dengue fever can cause liver inflammation, decreased immune system due to a viral infection. Meniran (Phyllanthus niruri) is often used in herbal medicine. Medical research on meniran is still continuing, especially itsÂ Â effect on the defense mechanism and liver inflammation which always decreasing during the fever. Â Keywords : Meniran, DHF, falvanoid, filatin, hepofilatin