bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of TSLC1, a candidate tumor suppressor gene mapped to chromosome 11q23, is downregulated in unfavorable neuroblastoma without promoter hypermethylation
Authors: Ando Kiyohiro , Ohira Miki , Ozaki Toshinori , Nakagawa Atsuko , Akazawa Kohei , Suenaga Yusuke , Nakamura Yohko , Koda Tadayuki , Kamijo Takehiko , Murakami Yoshinori , Nakagawara Akira 
Published: August 2008

AbstractAlthough it has been well documented that loss of human chromosome 11q is frequently observed in primary neuroblastomas, the smallest region of overlap (SRO) has not yet been precisely identified. Previously, we performed array‐comparative genomic hybridization (array‐CGH) analysis for 236 primary neuroblastomas to search for genomic aberrations with high‐resolution. In our study, we have identified the SRO of deletion (10‐Mb or less) at 11q23. Within this region, there exists a TSLC1/IGSF4/CADM1 gene (Tumor suppressor in lung cancer 1/Immunoglobulin superfamily 4/Cell adhesion molecule 1), which has been identified as a putative tumor suppressor gene for lung and some other cancers. Consistent with previous observations, we have found that 35% of primary neuroblastomas harbor loss of heterozygosity (LOH) on TSLC1 locus. In contrast to other cancers, we could not detect the hypermethylation in its promoter region in primary neuroblastomas as well as neuroblastoma‐derived cell lines. The clinicopathological analysis demonstrated that TSLC1 expression levels significantly correlate with stage, Shimada's pathological classification, MYCN amplification status, TrkA expression levels and DNA index in primary neuroblastomas. The immunohistochemical analysis showed that TSLC1 is remarkably reduced in unfavorable neuroblastomas. Furthermore, decreased expression levels of TSLC1 were significantly associated with a poor prognosis in 108 patients with neuroblastoma. Additionally, TSLC1 reduced cell proliferation in human neuroblastoma SH‐SY5Y cells. Collectively, our present findings suggest that TSLC1 acts as a candidate tumor suppressor gene for neuroblastoma. © 2008 Wiley‐Liss, Inc.
Rcl is a novel ETV1/ER81 target gene upregulated in breast tumors
Authors: Shin Sook , Bosc Denis G. , Ingle James N. , Spelsberg Thomas C. , Janknecht Ralf 
Published: August 2008

AbstractETV1 (ER81) is a transcription factor that can be activated by HER2/Neu, a proto‐oncoprotein often overexpressed in metastatic breast tumors. Here, we demonstrate that ETV1 downregulation suppresses proliferation of HER2/Neu‐positive MDA‐MB‐231 breast cancer cells in vitro and tumor formation in vivo, proving for the first time the existence of a critical role of ETV1 in breast cancer cell physiology. A screen for novel ETV1 target genes hinted at Rcl, an enzyme involved in nucleotide metabolism. To characterize the human Rcl gene, we cloned its promoter and found that ETV1 and HER2/Neu cooperated in activating the Rcl promoter, whereas a dominant‐negative ETV1 molecule suppressed the Rcl promoter. Moreover, ETV1 and HER2/Neu synergized to upregulate the endogenous Rcl gene. ETV1 also bound to the Rcl promoter in vivo, indicating that Rcl is a bona fide target gene of ETV1. Hybridization of Rcl cDNA to a breast cancer array revealed that Rcl is overexpressed in ∼40% of all breast tumors. Importantly, its expression significantly escalates with increasing tumor grade, strongly implicating that Rcl contributes to breast tumorigenesis. Since joint overexpression of Rcl with vascular endothelial growth factor, another target gene of ETV1, has been shown to induce tumor formation, Rcl may be one crucial effector of ETV1 and HER2/Neu in breast tumors. Furthermore, given its expression pattern and enzymatic function in nucleotide metabolism, Rcl presents itself as a novel target in breast cancer therapy via modulation of its activity by small molecule drugs. J. Cell. Biochem. 105: 866–874, 2008. © 2008 Wiley‐Liss, Inc.
ERK activation and nuclear signaling induced by the loss of cell/matrix adhesion stimulates anchorage‐independent growth of ovarian cancer cells
Authors: Al‐Ayoubi Adnan , Tarcsafalvi Adel , Zheng Hui , Sakati Wayne , Eblen Scott T. 
Published: August 2008

AbstractOvarian cancer metastasis involves the sloughing of epithelial cells from the ovary into the peritoneal cavity, where the cells can survive and proliferate in peritoneal ascites under anchorage‐independent conditions. For normal epithelial cells and fibroblasts, cell adhesion to the extracellular matrix is required to prevent apoptosis and for proper activation and nuclear signaling of the ERK MAP kinase. The mechanisms of ERK regulation by adhesion have been determined by our lab and others. In this report, we elucidate a novel means of ERK regulation by cellular adhesion in ovarian cancer cells. We demonstrate that ERK and its activator MEK are robustly stimulated after cell detachment from a substratum in several ovarian cancer cell lines, but not a benign ovarian cell line, independent of serum and FAK or PAK activity. MEK and ERK activation was sustained for 48 h after detachment, while activation by serum or growth factors in adherent cells was transient. Re‐attachment of suspended ovarian cells to fibronectin restored basal levels of MEK and ERK activity. ERK activity in suspended cells was dynamically controlled through an autocrine stimulatory pathway and prevalent phosphatase activity. Suspended cells demonstrated higher levels of ERK nuclear signaling to Elk1 compared to adherent cells. Inhibition of ERK activation with the MEK inhibitor U0126 had minor effects on adherent cell growth, but greatly decreased growth in soft agar. These data demonstrate a unique regulation of ERK by cellular adhesion and suggest a mechanism by which ERK may regulate anchorage‐independent growth of metastatic ovarian cancer cells. J. Cell. Biochem. 105: 875–884, 2008. © 2008 Wiley‐Liss, Inc.
Distinct roles of the three Akt isoforms in lactogenic differentiation and involution
Authors: Maroulakou Ioanna G. , Oemler William , Naber Stephen P. , Klebba Ina , Kuperwasser Charlotte , Tsichlis Philip N. 
Published: August 2008

AbstractThe three Akt isoforms differ in their ability to transduce oncogenic signals initiated by the Neu and PyMT oncogenes in mammary epithelia. As a result, ablation of Akt1 inhibits and ablation of Akt2 accelerates mammary tumor development by both oncogenes, while ablation of Akt3 is phenotypically almost neutral. Since the risk of breast cancer development in humans correlates with multiple late pregnancies, we embarked on a study to determine whether individual Akt isoforms also differ in their ability to transduce hormonal and growth factor signals during pregnancy, lactation and post‐lactation involution. The results showed that the ablation of Akt1 delays the differentiation of the mammary epithelia during pregnancy and lactation, and that the ablation of Akt2 has the opposite effect. Finally, ablation of Akt3 results in minor defects, but its phenotype is closer to that of the wild type mice. Whereas the phenotype of the Akt1 ablation is cell autonomous, that of Akt2 is not. The ablation of Akt1 promotes apoptosis and accelerates involution, whereas the ablation of Akt2 inhibits apoptosis and delays involution. Mammary gland differentiation during pregnancy depends on the phosphorylation of Stat5a, which is induced by prolactin, a hormone that generates signals transduced via Akt. Here we show that the ablation of Akt1, but not the ablation of Akt2 or Akt3 interferes with the phosphorylation of Stat5a during late pregnancy and lactation. We conclude that the three Akt isoforms have different roles in mammary gland differentiation during pregnancy and this may reflect differences in hormonal signaling. J. Cell. Physiol. 217: 468–477, 2008. © 2008 Wiley‐Liss, Inc.
Drugs, carrier solutions and temperature in hyperthermic intraperitoneal chemotherapy
Authors: Kusamura Shigeki , Dominique Elias , Baratti Dario , Younan Rami , Deraco Marcello 
Published: August 2008

AbstractAt the Fifth International Workshop on Peritoneal Surface Malignancy, in Milan, the consensus on technical aspects of cytoreductive surgery (CRS) for peritoneal surface malignancy was obtained through the Delphi process. Conflicting points concerning drugs, carrier solution and optimal temperature for hyperthermic intraperitoneal chemotherapy (HIPEC) were discussed. J. Surg. Oncol. 2008;98:247–252. © 2008 Wiley‐Liss, Inc.
Locoregional treatment of peritoneal carcinomatosis from gastric cancer
Authors: Bozzetti F. , Yu W. , Baratti Dario , Kusamura Shigeki , Deraco Marcello 
Published: August 2008

AbstractThe authors reviewed the natural history and the main features of the peritoneal carcinomatosis from gastric cancer briefly and analyzed the pertinent literature concerning the locoregional modalities for prevention and for treatment. Results of the web based voting by experts were also summarized. As regards the peritoneal perfusion with cytotoxic drugs with or without hyperthermia for preventing peritoneal carcinomatosis in high risk patients, there are some randomized clinical trials and one meta‐analysis supporting a benefit of the procedure. However, disparity in methodology (drugs, dosage, duration of the treatment, addition of hyperthermia, etc.) precludes the adoption of a shared protocol to be used in the clinical practice in high risk patients. Once the peritoneal carcinomatosis is established, the approach reported in literature is the peritonectomy associated with hyperthermic perfusion. However, data supporting benefits are scanty, and limited to few centers with a specific experience in this field. With regard to the main questions addressed to the experts' panel and concerning the indications for treatment and methodology, there was a general consistency among the experts and agreement with the findings of the literature. The need for a large multicenter trial to confirm the benefit and risk of intraperitoneal chemotherapy was recognized by both the experts and the authors. J. Surg. Oncol. 2008;98:273–276. © 2008 Wiley‐Liss, Inc.
Consensus statement on peritoneal mesothelioma
Authors: Deraco Marcello , Bartlett David , Kusamura Shigeki , Baratti Dario 
Published: August 2008

AbstractDiffuse malignant peritoneal mesothelioma (DMPM) has been traditionally regarded as a rapidly lethal disease. Recently, several independent prospective trials have reported improved survival with an intensive loco‐regional treatment strategy including cytoreductive surgery (CRS) along with peri‐operative intra‐peritoneal chemotherapy (PIC). However, most of the surgical data comes from mono‐institutional phase I or II studies and there is a broad range of variability regarding inclusion criteria, cytoreductive surgical procedures, drugs, temperatures and methods of delivering the heated chemotherapy (open vs. closed abdomen). This manuscript critically analyze and discuss the results of a group of health care providers trying to achieve a consensus statement in the management of this group of patients. The main conflicting points regarding preoperative evaluation, patient eligibility, combined treatment methodology, postoperative follow‐up and future investigational perspectives were summarized as a list of multiple‐choice questions. A questionnaire was placed on the website of the “5th International Workshop on Peritoneal Surface Malignancies” and the group members voted via internet. The results were presented for further debate during a dedicated session of the Workshop. The general treatment guidelines and future investigational perspectives were defined. J. Surg. Oncol. 2008;98:268–272. © 2008 Wiley‐Liss, Inc.
The Fifth International Workshop on Peritoneal Surface Malignancy (Milan, Italy, December 4–6, 2006): methodology of disease‐specific consensus
Authors: Baratti Dario , Kusamura Shigeki , Deraco Marcello 
Published: August 2008

AbstractPeritoneal surface malignancies (PSM) have been traditionally regarded as uniformly terminal conditions. The combination of cyto‐reductive surgery and perioperative intraperitoneal chemotherapy has changed PSM management from palliation to possible cure. Due to the inherent differences in biological and clinical behavior, the optimal adaptation of comprehensive treatment to each PSM is still a matter of debate. A session of “The Fifth International Workshop on Peritoneal Surface Malignancy” (Milan, Italy, December 4–6, 2006) was committed to reach a consensus pertaining to conceptual and technical aspects of the loco‐regional treatment of each PSM. The consensus developing process was based on principles of the Delphi method. A total of 103 international experts from 17 countries were included in six Working Groups (WG) for each of the following PSM: peritoneal mesothelioma, abdominal sarcomatosis, carcinomatosis of gastric, colo‐rectal, appendiceal, and ovarian origin. Evidence reports were written by the respective WG. The main conflicting points (CP) regarding preoperative evaluation, patient eligibility, combined treatment methodology, postoperative follow‐up and future investigational perspectives were summarized as a list of multiple‐choice questions. Overall, 160 CP were identified. A consensus ≥51% of voters favoring one option was reached in 143/160 CP (89.4%). The general treatment guidelines and future investigational perspectives were defined. J. Surg. Oncol. 2008;98:258–262. © 2008 Wiley‐Liss, Inc.
Technical aspects of cytoreductive surgery
Authors: Kusamura Shigeki , O'Dwyer Sarah T. , Baratti Dario , Younan Rami , Deraco Marcello 
Published: August 2008

AbstractAt the Fifth International Workshop on Peritoneal Surface Malignancy, in Milan, the consensus on technical aspects of cytoreductive surgery (CRS) for peritoneal surface malignancy was obtained through the Delphi process. Five conflicting points were discussed: radicality of the peritonectomy procedure, cytoreduction of neoplastic nodules <2.5 mm, the timing of bowel anastomoses in relation to hyperthermic intraperitoneal chemotherapy (HIPEC) and indications of protective ostomies. According to the panel of experts a partial parietal peritonectomy restricted to the macroscopically involved regions could be indicated in all listed clinical conditions with the exception of peritoneal mesothelioma. No expert was of the opinion that a radical parietal peritonectomy is advisable irrespective of the disease being treated. All the experts agreed that electrovaporization of small (<2.5 mm) non‐infiltrating metastatic nodules in the mesentery would be appropriate, even if theoretically the HIPEC affords microscopic cytoreduction. The panel also agreed that in the closed technique for HIPEC administration the intestinal anastomoses should be fashioned after completion of the perfusion. Finally when considering the place for protective ostomies the experts voted for a flexible approach allowing the surgeon to exercise discretion for individual patients. J. Surg. Oncol. 2008;98:232–236. © 2008 Wiley‐Liss, Inc.
Corroborating evidence of posttraumatic growth
Authors: Shakespeare‐Finch Jane , Enders Tracey 
Published: August 2008

AbstractOver the last decade, a healthy shift in the trauma literature has meant that published theory and research in the posttraumatic growth (PTG) domain has burgeoned, but the validity of the self‐report questionnaires used to measure PTG has recently been criticized. Corroboration of these subjective reports by an observer would provide convergent validity. Therefore, this study's aim was to validate the Posttraumatic Growth Inventory (PTGI; Tedeschi & Calhoun, 1996). Sixty‐one trauma survivors and 61 paired significant others completed the PTGI. A significant correlation was reported between the total PTGI scores of the trauma survivors and the corroborating significant others, supporting the use of the PTGI as an appropriate measure of positive posttrauma changes.
Spanish and English versions of the PTSD Checklist–Civilian version (PCL‐C): Testing for differential item functioning
Authors: Miles Jeremy N. V. , Marshall Grant N. , Schell Terry L. 
Published: August 2008

AbstractInterpretation of ethnic differences in PTSD is predicated on demonstration that differences are not due to measurement bias. This is difficult when multiple languages are used in the assessment. This study used confirmatory factor analysis to examine possible differential item functioning (DIF) across English and Spanish versions of the PTSD Checklist‐Civilian Version (PCL‐C). Data were derived from two assessments of Hispanics (Ns = 304, 213), who were hospitalized with physical injuries. After correction for multiple testing, univariate tests revealed no statistically significant DIF effects; multivariate tests revealed some indication of DIF at the initial assessment only. This bias was inconsistent across waves and unlikely to be substantively consequential, indicating that the two versions of the PCL‐C were generally equivalent.
Association of objective measures of trauma exposure from motor vehicle accidents and posttraumatic stress symptoms
Authors: Fujita Goro , Nishida Yasushi 
Published: August 2008

AbstractAssociations of objective measures of trauma exposure with psychological sequelae following motor vehicle accidents (MVA) were examined in a Japanese population. Impact and injury severity of 93 MVA victims was assessed using on‐the‐scene in‐depth investigations measured by the Injury Severity Score (ISS), barrier equivalent speed (BES), and change in velocity during the impact (Delta‐v). Results showed that ISS, BES, and Delta‐v were not related to posttraumatic stress symptoms (PTSS) or psychiatric symptoms at 5 and 14 months after the MVA. Subjective measures (e.g., perceived life risk, persistent medical problems) were significantly related to psychological sequelae. These findings suggest that the objective measures of trauma exposure are not associated directly with PTSS or psychiatric symptoms after an MVA.
Analysis of major proteins and fat fractions associated with mare's milk fat globules
Authors: Barello Cristina , Garoffo Lorenza Perono , Montorfano Gigliola , Zava Stefania , Berra Bruno , Conti Amedeo , Giuffrida Maria Gabriella 
Published: August 2008

AbstractAlthough several studies have aimed to identify mare's milk proteins, only the major whey proteins and some caseins have yet been characterized. Incomplete sequencing of the equine genome and the difficulty of recovering highly hydrophobic proteins mean that little is known to date about the proteins associated with milk fat globules, which have been shown to play an important role in newborns' defense mechanisms. The fat fraction, in particular the distribution of unsaturated fatty acids, has been more extensively studied, but complex lipids are only partially elucidated. This study reports a 2‐DE approach combined with a powerful method for de novo protein sequencing, and quali‐quantitative data on complex lipid composition determined by high performance TLC (HPTLC) and GC. The presence in mare's milk of long‐chain highly unsaturated fatty acids, and the evidence of close similarity between equine and human milk fat globule membrane proteins, support the use of mare's milk for human nutrition.
Knockouts of Se‐glutathione peroxidase‐1 and Cu,Zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice
Authors: Wang Xiaodan , Gillen Elizabeth A. , van der Meulen Marjolein C. H. , Lei Xin Gen 
Published: August 2008

AbstractThe objective of this study was to determine the impact of knockout of Cu,Zn‐superoxide dismutase (SOD1) and Se‐glutathione peroxidase‐1 (GPX1) on murine bone biomechanical properties. Femora samples were collected from wild‐type (WT), SOD1‐knockout [SOD1(–/–)] and GPX1‐knockout [GPX1(–/–)] female mice (9‐wk old, n = 7–8 per genotype) to assay for bone enzyme activities and mechanical properties in three point bending. Prior to testing, all mice were fed a torula yeast diet supplemented with 0.4 mg Se/kg as sodium selenite. Compared with the WT mice, SOD1(–/–) mice displayed a series of reductions (p < 0.05): 24% in body mass, 8% in femoral length, 43% in femoral structural strength, and 32% in bending stiffness. When differences in body size were accounted for, femoral failure moment in SOD1(–/–) mice remained lower (p < 0.05) than that of WT. Femoral tartrate resistant acid phosphatase activity in SOD1(–/–) was 47% greater (p < 0.05) than the WT. In contrast, GPX1(–/–) mice showed no significant differences in femoral mechanical properties from those of WT mice. In conclusion, knockout of SOD1 exerted a greater impact on femoral mechanical characteristics than that of GPX1 in growing mice.
Mechanisms of selenium chemoprevention and therapy in prostate cancer
Authors: Nadiminty Nagalakshmi , Gao Allen C. 
Published: August 2008

AbstractProstate cancer (PC) chemoprevention has generated considerable interest in the last decade and selenium and combinations of selenium have been recognized as one of the most efficacious chemopreventive agents against PC. This review focuses on a discussion of the knowledge hitherto gained about the mechanisms of action of the various in vitro and in vivo used selenium compounds and their effects on cellular processes and signaling pathways. We also describe the clinical and preclinical studies that have contributed enormously to the knowledge about dose, duration of exposure, and the chemical form of selenium effective in different scenarios. Even though the jury is still out about whether selenium can be used as a chemopreventive agent in the clinic and whether studies with cell lines and populations at low, medium, or high risk can adequately represent the physiological behavior of this micronutrient, it can safely be said to offer the most diverse spectrum of protective effects against this particular type of cancer which may augur well for its future as a chemopreventive agent.
Anthocyanins and their metabolites are weak inhibitors of cytochrome P450 3A4
Authors: Dreiseitel Andrea , Schreier Peter , Oehme Anett , Locher Sanja , Hajak Goeran , Sand Philipp G. 
Published: August 2008

AbstractThe cytochrome P450 enzyme cytochrome P450 3A4 (CYP3A4) controls the metabolism of about 60% of all drugs, and its inhibition may dramatically affect drug safety. Modulation of cytochrome P450 activity has been observed by constituents of fruit extracts including several flavonoids. The present investigation addresses CYP3A4 inhibition by anthocyanins, their aglycons, proanthocyanidins, and phenolic metabolites using a chemiluminescent assay. Test compounds inhibited CYP3A4 activity in a concentration‐dependent manner featuring IC50values from 12.2 up to 7,842 μM. In the order of decreasing effect size, anthocyanidins were followed by anthocyanins, proanthocyanidins, and phenolic acids. When compared to earlier data on furanocoumarins from grapefruit extract, the inhibitory activity of tested anthocyanins, and anthocyanidins was shown to be about 10 000‐fold weaker, and was negligible for phenolic acids (>100 000‐fold weaker). Future studies are invited to address effects of the above flavonoids on other CYP isoforms for more detailed toxicity profiles.