bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the degradation performance of novel molecularly imprinted Nd-TiO2 for oxytetracycline hydrochloride
Authors: Huang Zhongwei , Wang Bohai , Liu Bei , Liu Xian , Zhu Lei , Wang Xun 
Published: April 2024

Abstract
The objective of the study was to synthesize a novel photocatalyst, referred to as molecularly imprinted Nd-TiO2 (MIP-Nd-TiO2), through the sol-gel method. It rapidly identifies and breaks down oxytetracycline hydrochloride (OTC). By manipulating the doping ratio of Nd, incorporating imprinted molecules, and regulating the calcination conditions, the molar ratio of Ti to Nd was optimized to 100:1.25, the mass of added OTC was maintained at 0.15 g, and MIP-Nd-TiO2 was synthesized by calcining it for 2 h at 500 had the best degradation performance. Furthermore, the impact of these variables on the photocatalytic efficiency of the MIP-Nd-TiO2 catalysts was thoroughly investigated by varying the concentrations of pollutants and pH. The materials underwent evaluation employing precise characterization techniques, including, XPS, BET, and FT-IR among others. The findings from the photocatalytic experiments revealed that the degradation rates of OTC by MIP-Nd-TiO2, Nd-TiO2, and TiO2 consisted of 91.97%, 76.47%, and 64.34%, correspondingly, after dark adsorption equilibrium and followed by irradiation with 365 nm UV light for 30 min. Specifically, in just 40 minutes, the MIP-Nd- TiO2 adsorption-photocatalytic synergy was capable of achieving a 93.14% degradation rate for OTC. Moreover, an investigation was conducted into the photocatalytic and adsorption selectivity of MIP-Nd-TiO2. To conclude, this study has illustrated the viability of employing photocatalysis and molecular imprinting in tandem, which can be an extremely efficient technique for treating OTC in wastewater.
Proteomic study of secretomes from cellular agriculture for milk production
Authors: Che Jing , Nielsen Søren Drud-Heydary , Sattari Zahra , Yue Yuan , Purup Stig , Poulsen Nina Aagaard , Larsen Lotte Bach 
Published: April 2024

Abstract
Cultivation of bovine mammary epithelial cells (bMECs) is regarded as a potential future source of milk constituents; however, there is a research gap concerning the investigation of compositional similarity between such secretomes and milk, and furthermore, how culture conditions can be optimized to make it a feasible strategy for potential milk constituents production. To perform such an initial, detailed quantitative proteomic study of secretomes from in vitro grown bMECs, these were after isolation cultured on Matrigel®-coated inserts in a trans-well system. Cells were treated with or without prolactin at the basolateral sides, and the secretomes together with media from the apical side were collected and subsequently compositionally characterized by their protein contents and label-free quantitative proteomics. Using sensitive nLC-timsTOF Pro MS/MS, more than 500 proteins were identified, though in very low total concentrations, across proteomes from the four secretomes collected from prolactin- or non-prolactin-treated cells. The proteins included many cellular proteins and characteristic milk proteins, but with caseins accounting for less than 1% of total protein abundance. Secretomes from prolactin-treated bMECs showed significant (P < 0.05) up-regulation of 351 different proteins, including milk proteins such as αS1-casein, κ-casein, β-lactoglobulin, lactotranferrin, and osteopontin, indicating a positive regulation of lactation with prolactin treatment. These findings elucidate a potential for future cellular agriculture using in vitro cultured bMECs for the production of milk constituents, together with challenges, such as upscale and sufficient lactogenesis.
A Liver Transplant Patient Experienced Deterioration of Renal Function and Rhabdomyolysis after Taking Sirolimus: A Case report
Authors: Gao Chang , Chen Zhi-Yu , Ma Liang , Gou Shen-Ju 
Published: April 2024

Abstract

The occurrence of renal impairment and rhabdomyolysis in transplant patients taking sirolimus was very rare. We report a case of a 54-year-old male who received liver transplantation and was initially treated with tacrolimus, mycophenolate mofetil and glucocorticoids for immunosuppression. After developing renal dysfunction, tacrolimus was replaced with sirolimus. However, one month after taking sirolimus, the patient's renal function continued to deteriorate, and rhabdomyolysis developed one and a half months later. Serum analysis indicated high sirolimus concentration, while renal histopathology revealed acute tubular injury and interstitial arteriopathy. After reducing the dosage of sirolimus, the patient's creatine kinase levels returned to normal and renal function improved compared to before. 2 years after hospital discharge, the patient's renal function further recovered. This case highlights the importance of monitoring sirolimus blood concentration in clinical practice, as elevated drug concentrations can lead to renal dysfunction and rhabdomyolysis as adverse reactions. Further investigation into the pathogenic mechanisms of sirolimus-induced renal dysfunction and rhabdomyolysis may contribute to clinical practice.
Investigation of the expression pattern of defense-related genes in strawberry fruits in interaction with Bacillus spp. and Colletotrichum nymphaeae
Authors: Alijani Zahra , Amini Jahanshir , Bahramnejad Bahman 
Published: April 2024

Abstract

This research investigated the antifungal properties of the endophytic bacterium Bacillus spp. (ABN14), isolated from Fragaria × ananassa peduncle, in interaction with Colletotrichum nymphaeae on defense gene expression, defense-related enzymes, and the alteration of the metabolite profile of strawberry fruits. Statistical methods represented considerable differences in the expression of defense genes (PR5, PR10, and WRKY) at all time points (zero, 24, 72, and 96 hours) after the inoculation of the fungal pathogen in all treatments. Furthermore, strawberry fruits infected by the fungal pathogen, following exposure to the biological control agent, exhibited varying the amount of catalase, peroxidase, polyphenol oxidases, superoxide dismutase, and phenylalanine ammonia-lyase enzymes compared to those from other treatments at different times post-inoculation. Additionally, different treatments exerted various effects on the metabolite profile of strawberry fruits, leading to the production of diverse metabolites.
How post-fire succession shapes psammophilous ground-dwelling assemblages: effect of wildfire and alteration in food composition.
Authors: Stočes Dominik , Šipoš Jan , Špoula Jakub , Kula Emanuel 
Published: April 2024

Abstract
Psammophilous beetles, a group endangered in temperate ecosystems, are mainly threatened by habitat loss. These beetles thrive in areas formed by the weathering of marine or fluvial sediments. Historically, practices such as slash-and-burn method or litter raking have exposed and destabilized sediments. In Central Europe, the Scots pine (Pinus sylvestris) has been used to stabilize sand dunes, improve soil composition and create shadier environments. This has led to the degradation of psammophilous communities and a decline in biodiversity. Our study examines whether fire can help restore and maintain psammophilous beetle populations.
Our research focused on two aspects: (i) the long-term effects of wildfire in 2012 on beetles (Coleoptera: Carabidae) in the "Moravská Sahara–Bzenec district" of South Moravia (period 2013–2021), and (ii) the effects of environmental factors, influenced by the aforementioned wildfire on the distribution of these beetles in the period 2016 to 2021.
The study compared species composition, life-history traits, and functional diversity between three forest stages: (i) newly disturbed areas with Coarse Wood Debris (CWD) left to naturally decay; (ii) semi-decayed mid-aged stands and (iii) reference mid-aged stands.
Our findings indicate that fire was important factor influencing species assemblages. Interestingly, post-fire changes in food availability emerged as the second important factor, leading to the formation of two distinct species clusters: granivorous species thrived on site dominated by graminoids, and site with accumulated CWD was attracted predominantly by carnivorous species.
Implications for insect conservation: We suggest that fire management is an effective strategy for conserving and enhancing the diversity of post-fire early-successional beetle assemblages in areas where these species exist. Consequently, the increase in forest canopy closure in these habitats should not be solely regarded as regeneration or a return to a natural state. Instead, it should be considered a potential threat to their richness and diversity.
Development of the Compassionate Technology Scale for Professionals (CTS-P): Value Driven Evaluation of Digital Mental Health Interventions
Authors: Lotringen Charlotte M. van , Klooster Peter M. ten , Austin Judith , Westerhof Gerben J. , Kelders Saskia M. , Noordzij Matthijs L. 
Published: April 2024

Abstract
Background: Compassion is an essential and beneficial value in mental healthcare. However, how digital mental health interventions influence compassion in treatment has not been systematically investigated, due to the lack of appropriate measurement instruments. To address this gap, we developed the Compassionate Technology Scale for Professionals (CTS-P), aimed at mental health professionals.
Methods: We used Q-methodology, a method that combines quantitative and qualitative analysis to explore shared viewpoints on a particular topic, to select and refine items. Participants were 15 professionals from different areas of mental healthcare. In individual sessions, while thinking aloud, they sorted 35 statements on relevance for evaluating technology use on compassion. The statements were based on a scientific conceptualization of compassion with five elements. The sorting task was followed by a short interview to explore participants’ associations with compassion and technology.
Results: With by-person factor analysis, we found three different viewpoints among participants, all with eigenvalues >1 and with a total explained variance of 63.02%. We selected prioritized items of each viewpoint and for each theoretical element of compassion, resulting in a full scale (15 items) and a short scale (3 items). Based on qualitative input from participants, the scale was adapted to clarify its focus and the wording of items. While thinking aloud, participants shared benefits and critical notes regarding technology and compassion.
Discussion: Together with key stakeholders, we developed the CTS-P. Most participants prioritized the potential of technology to obtain more information and be closer to their client in facilitating compassion. The main critical note participants had was that technology is not necessary to support compassion at the therapist’s experiential level. This emphasizes the need to further explore how mental health professionals and technology can complement each other in a system of compassionate care. Future research should explore the factor structure, validity, and reliability of the scale through psychometric validation.
Conclusions: The CTS-P can make the extent to which technology for mental healthcare influences elements of compassion measurable and comparable. The scale can prove useful for prioritizing the value of compassion in both the development and use of digital mental health interventions.
Acquired tracheomalacia in a 9-year-old patient with pectus carinatum following modified Abramson technique
Authors: Song Tieniu , zhang Shaobo , Jiang Peng , Zhou Hui 
Published: April 2024

Abstract
The minimally invasive repair of pectus carinatum (MIRPC) was first introduced by Abramson in 2005. With the increasing popularity and refinement of such technique, the application range has also expanded to include not only the chondrogladiolar type but also the chondromanubrial type. The presence of adequate flexibility in the chest wall has been a prerequisite for applying MIRPC, however, the assessment of sternum flexibility has predominantly relied on subjective compression tests.We report a case of acquired tracheomalacia in a 9-year-old boy with PC following MIRPC, whose chest wall demonstrated slightly increased rigidity while remaining malleable for shaping during the preoperative compression test. The patient's condition improved after treatment and he was subsequently discharged; nevertheless, the entire process of diagnosis and treatment still carries significant cautionary and instructive implications.
Identification and characterization of new Cys2-His2 zinc finger transcription factors, ZCTs, in regulating alkaloid biosynthesis in Catharanthus roseus
Authors: Traverse Krystyna F. , Breselge Samuel , Trautman Juliet , Dee Amanda , Wang Jie , Childs Kevin , Lee-Parsons Carolyn 
Published: April 2024

Abstract

Catharanthus roseus, commonly known as the Madagascar periwinkle, is the sole known producer of several medicinal terpenoid indole alkaloids (TIAs), including the anti-cancer compounds vinblastine and vincristine. While the enzymatic steps of the pathway have been elucidated, an understanding of its regulation is still emerging. The present study characterizes an important family of Cys2-His2 zinc finger transcription factors known as Zinc finger Catharanthus Transcription factors (ZCTs). We identified three new members of the ZCT family, named ZCT4, ZCT5, and ZCT6, in addition to the previously characterized, homologous ZCT1, ZCT2, and ZCT3 in the C. roseus genome. We characterized the tissue-specific and jasmonate-responsive expression of the ZCTs and showed that ZCTs are regulators of the TIA pathway. The transient overexpression of ZCTs in seedlings significantly repressed the promoters of the terpenoid (pLAMT) and condensation branch (pSTR1) of the TIA pathway. ZCTs also significantly repressed and indirectly activated several promoters of the vindoline pathway. The expression of ZCTs (except ZCT5) increased with jasmonate in a dosage-dependent manner. We showed significant activation of the pZCT1 and pZCT3 promoters by de-repressed CrMYC2a, suggesting that the jasmonate-responsive expression of the ZCTs can be mediated by CrMYC2a. In summary, the C. roseus ZCTs are jasmonate-responsive, can be induced by CrMYC2a, and can act as significant regulators of the TIA pathway when highly expressed.
The effect of music on patient anxiety undergoing bronchoscopy: A randomized controlled trial
Authors: Kacem Amani , Aissa Sana , Jomaa Wassim , Chebil Dhekra , Jazia Rahma Ben , Kharrat Imen , Benzarti Wafa , Knaz Asma , bdira Baraa Ben , Gargouri Imen , Ayechi Jihen , Rihab Moncer , Anis Maatallah , Ahmed Abdelghani 
Published: April 2024

Abstract
Background: Bronchoscopy is a fundamental diagnostic and therapeutic examination with rare complications which is often associated to anxiety and discomfort.
The aim of our study is to evaluate the effect of music on anxiety levels among patients undergoing bronchoscopy in the pneumology department
Methods: we conducted a prospective, randomized, investigator-blinded, controlled trial performed in two university hospitals. The patients were randomized into two groups: control group who had preselected classic Tunisian music during bronchoscopy and experimental group who had bronchoscopy without music. we assessed anxiety level using Spilberger’s State Anxiety inventory (S-STAI) and comfort level using visual analogic scale (VAS). Vital signs and side effects during bronchoscopy were also assessed.
Results: 136 patients were equally assigned into the two groups. the anxiety levels before and after bronchoscopy were similar into the two groups. The music had no effect on anxiety level using the S-SATI. There was no significant difference between the two groups regarding side effects and vital signs during bronchoscopy. There was a significant effect of music on mean change of VAS score (p=0.018).
Conclusion: The music improved comfort level of patients undergoing bronchoscopy.
This study was retrospectively registered in the pan African Clinical Trial Registry with the trial number PACTR202309620440045
Integrative analyses of hypoxia-related genes and mechanisms associated with Allergic Rhinitis
Authors: Shao Shiyun , Wei Kunchen , Feng Xiao , Huang Guanhui 
Published: April 2024

Abstract

Background In the realm of immunological disorders, allergic rhinitis (AR) persists as a prevalent condition, yet its molecular underpinnings remain only partially deciphered, necessitating deeper exploration. This study pioneers in bridging this knowledge gap, unveiling intricate molecular markers and pathways pivotal to AR's pathophysiology, thereby steering the scientific community towards novel diagnostic and prognostic frontiers. Employing rigorous bioinformatics analyses, similar to methodologies applied in studies on endometriosis and age-related macular degeneration, we delved into the molecular landscape, identifying 21 hypoxia-related differential expression genes (HRDEGs) and constructing a robust LASSO diagnostic model, a methodology that stands out for its precision in capturing clinical heterogeneity.Methods Our approach encompassed a comprehensive analysis of differential gene expressions, focusing particularly on HRDEGs, and their subsequent integration into a logistic regression model to ascertain their diagnostic and prognostic efficacy. Key findings revealed a high expression of genes such as CPT1C and MMP1 in the AR group, underscoring their significance in AR's molecular signature. Furthermore, the constructed LASSO model demonstrated high accuracy, highlighting genes like CPT1C, CWF19L1, MED17, and MMP1 as reliable biomarkers.Results Interestingly, the study also unearthed a nuanced interplay between AR and other systemic conditions, suggesting that the molecular mechanisms underlying allergic inflammation could influence the pathophysiology of various respiratory diseases3. These insights not only contribute to the academic discourse but also hold profound therapeutic potential, particularly in the realm of personalized medicine.Conclusions In conclusion, this research illuminates the molecular complexities of AR, offering substantial evidence for the involvement of specific genes and pathways in its pathogenesis. The implications of these discoveries are far-reaching, promising to revolutionize AR management through more tailored therapeutic strategies and underscoring the need for further investigations in larger, more diverse cohorts.
Efficacy of Hyperbaric Oxygen Therapy in Central Retinal Artery Occlusion: A Comprehensive Evaluation Using Enhanced Depth Imaging Optical Coherence Tomography
Authors: Lee Jung Min , Choi Se Hyun , Jeon Gang Seok , Chang In Boem , Wang Soon Joo , Hong In Hwan 
Published: April 2024

Abstract

Purpose
This study aimed to assess the efficacy of hyperbaric oxygen therapy (HBOT) in patients with central retinal artery occlusion (CRAO) by analyzing changes in visual acuity (VA) and enhanced depth imaging optical coherence tomography (EDI-OCT) parameters.
Method
Medical records of patients diagnosed with unilateral CRAO were analyzed. Patients were divided into two groups: those receiving HBOT in addition to standard treatment and those receiving standard treatment alone. Baseline characteristics were compared, and VA and OCT parameters were evaluated at baseline and follow-up visits.
Results
A total of 50 eyes from 50 patients were included, with 29 eyes in the HBOT group and 21 eyes in the control group. HBOT-treated patients showed significant improvements in VA than control group throughout the follow-up period. Central choroidal thickness increased significantly in the HBOT group. The central choroid, central fovea, and outer retinal layer thickness in the HBOT group were significantly greater than those in the control group at the 6-month follow-up after treatment.
Conclusion
HBOT appears to be effective in improving VA and inducing favorable changes in OCT parameters in patients with CRAO. Preservation of the choroid and outer retinal layer may contribute to the significant visual recovery observed in HBOT-treated patients.
Real-word immunogenicity of a recombinant subunit COVID-19 vaccine and effectiveness against omicron infection: a prospective, multi-centre, longitudinal cohort study in Alzheimer's disease patients
Authors: Weng Youhong , Huang Yihua , Zhang Jiangtao , Wu Yahan , He Qing , Litchev Sofia , Wu Aimee , Ling Zongxin , Zhao Longyou , Liao Rongxian , Shao Li , Wang Minchao , Lai Yunru , Li Jie , Xu Yu , Gong Ruoyao , Zhang Zhihua , Wang Yuwei , Lu Shaohong , Lv Hao , Kong Qingming 
Published: April 2024

Abstract
Introduction Vaccination is an essential strategy against COVID-19 in the current era of emerging variants. This study evaluates the real-world immunogenicity and effectiveness of the recombinant subunit COVID-19 vaccine (Zifivax) in Alzheimer's disease (AD) patients.Methods 249 AD patients were enrolled in a multicentre, longitudinal cohort study. Levels of RBD-IgG, neutralization antibody activity, and cytokines were identified to evaluate the immune responses. Clinical outcomes were assessed within one month following Omicron infection..Results Following three doses, the vaccine induced a robust immune response, elevating neutralizing antibodies and activating T-cells. AD patients exhibited significantly higher humoral immune responses compared to unvaccinated counterparts. Following Omicron infection, unvaccinated patients experienced higher levels of Th1/Th2-type cytokines than vaccinated individuals. Vaccination correlated with increased survival rates and extended survival times after infection..Discussion The findings highlight the vaccine's efficacy in reducing severe illness, and preventing death in AD patients facing Omicron infection.
A Large-Scale Mixed Reality Stadium for Training Combination Tactics in Basketball
Authors: Saiki Hayato , Hirokawa Masakazu , Hassan Modar , Suzuki Kenji 
Published: April 2024

Abstract

This paper proposes a mixed reality (MR)-based large-scale environment for training combination tactics in basketball. This MR environment enables human players to practice various combination tactics such as ball passing and screens with virtual players overlaid in the real environment using a see-through head-mounted display (HMD). Positions of human players and the ball were tracked using an optical motion capture system and transmitted to a unity-based control system, facilitating quantitative and real-time evaluation of gameplay. The evaluation was conducted through coaching experiments using the MR system involving novice players, along with validation evaluation experiments between the developed environment and real space through skilled basketball players. The coaching experiment with novice learners demonstrated that the proposed MR environment was more effective than conventional methods for training combination tactics.Additionally, the validation experiment with skilled players showed no significant differences in player behavior between the proposed environment and the real space, suggesting that the observed coaching results could be transferrable to real courts. These results demonstrate the potential of MR technology in skill training involving multiplayer coordination.