bluuebunny/crossref_metadata_2025_split huggingface dataset:
EFFECTIVENESS OF ABSORPTION WELLS USING THE SUNJOTO METHOD TO CONTROL FLOODING IN THE VILLAGE OF NGRASEH BOJONEGORO
Authors: Mushthofa Mushthofa , Fadhila Fajrina Nur , Indriani Yulia , Faqih Nasyiin , Qomaruddin Mochammad 
Published: March 2024

Infiltration wells are a form of hydrological infrastructure used to manage rainwater. Infiltration wells are usually built as part of a rainwater management system to soak rainwater into the ground and reduce surface waterlogging as well as slow down surface runoff and allow rainwater to seep into the ground to renew aquifers or prevent waterlogging. Maintaining groundwater levels is an important step in sustainable water resource management. The aim of this research is to plan effective infiltration wells to replace rainwater catchment areas lost due to building construction. The planning of this infiltration well uses the Sunjoto method. From the research results, it was found that an effective infiltration well design was calculated using the Sunjoto method. With the data, each roof area of ​​the house has an optimum number of infiltration wells with a planned infiltration well diameter of 1 m with a depth of 2.5 m with an effective depth according to the calculations is as follows: 60 m2 number of 1 unit of absorption well with an optimum depth of 2.82 m, 80 m2 number of 2 units of absorption well with an optimum depth of 3.76 m, 100 m2 number of 2 units of absorption well with an optimum depth of 4.7 m, 120 m2 number of 2 units of infiltration wells with an optimum depth of 5.64 m, 140 m2 number of 3 units of absorption wells with an optimum depth of 6.58 m, 160 m2 number of 3 units of absorption wells with an optimum depth of 7.52 m, 180 m2 number of 3 units of absorption wells with an optimum depth of 8.46 m, 200 m2 total of 4 units of infiltration wells with an optimum depth of 9.4 m.
Potensi Aktivitas Sitotoksik Belimbing Wuluh (Averrhoa bilimbi L.) Pada Sel Kanker
Authors: Utami Widyaningrum , Saragih Elisa Br. , Andini Merilla 
Published: March 2024

Kemoterapi merupakan pengobatan yang efektif digunakan untuk terapi berbagai jenis sel kanker namun memiliki banyak efek samping. Belimbing wuluh (Averrhoa bilimbi L.) merupakan alternatif tanaman obat digunakan untuk pengobatan tradisional seperti antibakteri, antivirus, dan antikanker. A. bilimbi memiliki kandungan fenolik yang tinggi yang didunga memiliki aktivitas sitotoksik. Tujuan dari review ini adalah untuk mengetahui potensi Averrhoa bilimbi L sebagai agen antikanker. Pencarian literatur dilakukan dengan melalui database online Google Scholar dan PubMed tahun 2011-2022. Senyawa aktif yang diduga memiliki efek antikanker pada belimbing wuluh yaitu squalene dan asam protocatechuic. Beberapa ekstrak etanol belimbing wuluh terbukti berpotensi sebagai agen antikanker secara in vitro dengan kategori sitotoksik sedang (IC50 = 21 µg/mL - 200 µg/mL) pada sel kanker serviks (sel HeLa), sel kanker payudara MCF-7, dan sel kanker payudara non hormonal (MDA-MB-23). Sedangkan secara in vivo, Averrhoa bilimbi L. terbukti memiliki aktivitas antikanker sebagai antilimfoma pada mencit albino Swiss yang diinduksi DAL (Dalton’s asites Lymphoma) dan potensi apoptogenik yang signifikan terhadap sel EAC (Ehrlich ascites carcinoma) tikus. Maka dari itu, kajian ini menunjukkan potensi aktivitas Averrhoa bilimbi L. sebagai antikanker.
Molecular dynamics of the human RhD and RhAG blood group proteins
Authors: Floch Aline , Galochkina Tatiana , Pirenne France , Tournamille Christophe , de Brevern Alexandre G. 
Published: March 2024

Introduction: Blood group antigens of the RH system (formerly known as “Rhesus”) play an important role in transfusion medicine because of the severe haemolytic consequences of antibodies to these antigens. No crystal structure is available for RhD proteins with its partner RhAG, and the precise stoichiometry of the trimer complex remains unknown.Methods: To analyse their structural properties, the trimers formed by RhD and/or RhAG subunits were generated by protein modelling and molecular dynamics simulations were performed.Results: No major differences in structural behaviour were found between trimers of different compositions. The conformation of the subunits is relatively constant during molecular dynamics simulations, except for three large disordered loops.Discussion: This work makes it possible to propose a reasonable stoichiometry and demonstrates the potential of studying the structural behaviour of these proteins to investigate the hundreds of genetic variants relevant to transfusion medicine.
Combination of metagenomic next-generation sequencing and conventional tests unraveled pathogen profiles in infected patients undergoing allogeneic hematopoietic stem cell transplantation in Jilin Province of China
Authors: Zou Hongyan , Gao Sujun , Liu Xiaoliang , Liu Yong , Xiao Yunping , Li Ao , Jiang Yanfang 
Published: March 2024

BackgroundInfection is the main cause of death for patients after allogeneic hematopoietic stem cell transplantation (HSCT). However, pathogen profiles still have not been reported in detail due to their heterogeneity caused by geographic region.ObjectiveTo evaluate the performance of metagenomic next-generation sequencing (mNGS) and summarize regional pathogen profiles of infected patients after HSCT.MethodsFrom February 2021 to August 2022, 64 patients, admitted to the Department of Hematology of The First Hospital of Jilin University for HSCT and diagnosed as suspected infections, were retrospectively enrolled.ResultsA total of 38 patients were diagnosed as having infections, including bloodstream (n =17), pulmonary (n =16), central nervous system (CNS) (n =4), and chest (n =1) infections. Human betaherpesvirus 5 (CMV) was the most common pathogen in both bloodstream (n =10) and pulmonary (n =8) infections, while CNS (n =2) and chest (n =1) infections were mainly caused by Human gammaherpesvirus 4 (EBV). For bloodstream infection, Mycobacterium tuberculosis complex (n =3), Staphylococcus epidermidis (n =1), and Candida tropicalis (n =1) were also diagnosed as causative pathogens. Furthermore, mNGS combined with conventional tests can identify more causative pathogens with high sensitivity of 82.9% (95% CI 70.4-95.3%), and the total coincidence rate can reach up to 76.7% (95% CI 64.1-89.4%).ConclusionsOur findings emphasized the importance of mNGS in diagnosing, managing, and ruling out infections, and an era of more rapid, independent, and impartial diagnosis of infections after HSCT can be expected.
Is the leptin/BMI ratio a reliable biomarker for endometriosis?
Authors: Zyguła Aleksandra , Sankiewicz Anna , Sakowicz Agata , Dobrzyńska Ewa , Dakowicz Agnieszka , Mańka Grzegorz , Kiecka Mariusz , Spaczynski Robert , Piekarski Piotr , Banaszewska Beata , Jakimiuk Artur , Issat Tadeusz , Rokita Wojciech , Młodawski Jakub , Szubert Maria , Sieroszewski Piotr , Raba Grzegorz , Szczupak Kamil , Kluza Tomasz , Kluza Marek , Pierzyński Piotr , Wojtyla Cezary , Lipa Michal , Warzecha Damian , Wielgos Miroslaw , Cendrowski Krzysztof , Gorodkiewicz Ewa , Laudanski Piotr 
Published: March 2024

BackgroundThe aim of this study was to analyze the concentration of leptin in peritoneal fluid and plasma and to assess their role as potential biomarkers in the diagnosis of endometriosis.Materials &amp; methodsLeptin adjusted for BMI (leptin/BMI ratio) was measured using surface plasmon resonance imaging (SPRI) biosensors. Patients with suspected endometriosis were included in the study. Plasma was collected from 70 cases, and peritoneal fluid from 67 cases. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group and a control group (patients without endometriosis).ResultsLeptin/BMI ratio in plasma did not differ between women with endometriosis and the control group (0.7159 ± 0.259 vs 0.6992 ± 0.273, p= 0,7988). No significant differences were observed in peritoneal leptin/BMI ratio levels in patients with and without endometriosis (0.6206 ± 0.258 vs 0.6215 ± 0.264, p= 0,9896). Plasma and peritoneal leptin/BMI ratios were significantly lower in women with endometriosis - related primary infertility compared to women with endometriosis without primary infertility (0.640 ± 0.502 vs 0.878 ± 0.623, p &lt; 0.05). The difference was observed in case of primary infertility, but not in terms of the secondary one. No significant differences were noted between leptin/BMI ratio in the proliferative phase and the secretory phase (0.716 ± 0.252 vs 0.697 ± 0.288, p= 0,7785).ConclusionThe results of present study do not support the relevance of leptin concentration determination as a biomarker of the endometriosis. Due to the limited number of samples in the tested group, further studies are needed to confirm its role.
The design of an improved index system for frequency control in China Southern Power Grid
Authors: Kuo Xin , Hongxuan Zhang , Wenzu Wu , Jingpeng Chen , Qixing Liu 
Published: March 2024

Introduction: In order to dispatch frequency regulation resources in regional power grids efficiently and promote the development of spot markets, China Southern Power Grid (CSG) established the unified frequency regulation control area. However, the existing regional control performance standards (CPS) for evaluating the performance of frequency control in bulk power systems is no longer suitable for the unified frequency control mode.Method: This paper proposed an innovative frequency control performance standard, named tertiary control performance standards (TCPS) and used Gaussian mixture model (GMM) and folded normal distribution (FND) to describe the distributions of frequency deviations and power deviations of tie line.Result and Discussion: Based on these probability models, the parameters of the proposed TCPS can be determined optimized. Finally, case studies were carried out with the practical data from CSG and indicated that the parameters of proposed TCPS index could be calculated and improved the control performance for the real time power balance of the regional power grid with spot markets.
Efficacy of the induced pluripotent stem cell derived and engineered CD276-targeted CAR-NK cells against human esophageal squamous cell carcinoma
Authors: Lin Xiaolan , Guan Tian , Xu Yien , Li Yun , Lin Yanchun , Chen Shaobin , Chen Yuping , Wei Xiaolong , Li Dongsheng , Cui Yukun , Lin Yan , Sun Pingnan , Guo Jianmin , Li Congzhu , Gu Jiang , Yang Wei , Zeng Haoyu , Ma Changchun 
Published: March 2024

IntroductionChimeric antigen receptor natural killer (CAR-NK) cells have been found to be successful in treating hematologic malignancies and present potential for usage in solid tumors.MethodsIn this study, we created CD276-targeted CAR-expressing NK cells from pluripotent stem cells (iPSC CD276-targeted CAR-NK cells) and evaluated their cytotoxicity against esophageal squamous cell carcinoma (ESCC) using patient-specific organoid (PSO) models comprising of both CD276-positive and CD276-negative adjacent epithelium PSO models (normal control PSO, NC PSO) as well as primary culture of ESCC cell models. In addition, in vitro and in vivo models such as KYSE-150 were also examined. iPSC NK cells and NK-free media were used as the CAR-free and NK-free controls, respectively.ResultsThe positive CD276 staining was specifically detected on the ESCC membrane in 51.43% (54/105) of the patients of all stages, and in 51.35% (38/74) of stages III and IV. The iPS CD276-targeted CAR-NK cells, comparing with the iPS NK cells and the NK-free medium, exhibited specific and significant cytotoxic activity against CD276-positive ESCC PSO rather than CD276-negative NC PSO, and exhibited significant cytotoxicity against CD276-expressing cultured ESCC cells, as well as against CD276-expressing KYSE-150 in vitro and in BNDG mouse xenograft.DiscussionThe efficacy of the iPSC CD276-targeted CAR-NK cells demonstrated by their successful treatment of CD276-expressing ESCC in a multitude of pre-clinical models implied that they hold tremendous therapeutic potential for treating patients with CD276-expressing ESCC.
Transcriptomics in idiopathic pulmonary fibrosis unveiled: a new perspective from differentially expressed genes to therapeutic targets
Authors: Hu Wenzhong , Xu Yun 
Published: March 2024

BackgroundThe underlying molecular pathways of idiopathic pulmonary fibrosis (IPF), a progressive lung condition with a high death rate, are still mostly unknown. By using microarray datasets, this study aims to identify new genetic targets for IPF and provide light on the genetic factors that contribute to the development of IPF.MethodWe conducted a comprehensive analysis of three independent IPF datasets from the Gene Expression Omnibus (GEO) database, employing R software for data handling and normalization. Our evaluation of the relationships between differentially expressed genes (DEGs) and IPF included differential expression analysis, expression quantitative trait loci (eQTL) analysis, and Mendelian Randomization(MR) analyses. Additionally, we used Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to explore the functional roles and pathways of these genes. Finally, we validated the results obtained for the target genes.ResultsWe identified 486 highly expressed genes and 468 lowly expressed genes that play important roles in IPF. MR analysis identified six significantly co-expressed genes associated with IPF, specifically C12orf75, SPP1, ZG16B, LIN7A, PPP1R14A, and TLR2. These genes participate in essential biological processes and pathways, including macrophage activation and neural system regulation. Additionally, CIBERSORT analysis indicated a unique immune cell distribution in IPF, emphasized the significance of immunological processes in the disease. The MR analysis was consistent with the results of the analysis of variance in the validation cohort, which strengthens the reliability of our MR findings.ConclusionOur findings provide new insights into the molecular basis of IPF and highlight the promise of therapeutic interventions. They emphasize the potential of targeting specific molecular pathways for the treatment of IPF, laying the foundation for further research and clinical work.
The association between diverse serum folate with MAFLD and liver fibrosis based on NHANES 2017–2020
Authors: Cai Jiacheng , Chen Dahua , Luo Wenjing , Xu Feng , Feng Xiaofeng , Zhang Liangshun , Liu Huiwei , Shen Jianwei , Ye Hua 
Published: March 2024

BackgroundMetabolically Associated Fatty Liver Disease (MAFLD) marks a progression from the previous paradigm of Non-Alcoholic Fatty Liver Disease (NAFLD), presenting a redefined diagnostic framework that accentuates metabolic factors while recognizing non-alcoholic contributors. In our investigation, our principal aim was to scrutinize the conceivable correlation between diverse serum folate levels and the prevalence of MAFLD and liver fibrosis.MethodsIn our investigation, we conducted an extensive analysis utilizing data derived from the National Health and Nutrition Examination Survey (NHANES) across the years 2017–2020. We aimed to investigate the association between different serum folate concentrations and the prevalence of MAFLD and liver fibrosis by comprehensive multivariate analysis. This analytical approach considered various variables, encompassing sociodemographic characteristics, lifestyle factors, hypertension, and diabetes. By including these potential confounders in our analysis, we aimed to ensure the stability of the findings regarding the association between different serum folate concentrations and the development of MAFLD and liver fibrosis.ResultsIn our investigation, we utilized multiple linear regression models to thoroughly analyze the data, revealing noteworthy insights. Evidently, elevated levels of both total folate and 5-MTHF exhibited a distinct negative correlation with CAP, while 5-MTHF demonstrated a notable negative correlation with LSM. Furthermore, multiple logistic regression models were employed for an in-depth examination of the data. As the concentrations of total folate and 5-MTHF in the serum increased, a substantial decrease in the likelihood of MAFLD and liver fibrosis occurrence was observed.ConclusionThe findings of this investigation robustly suggest the prevalence of MAFLD and liver fibrosis decreased significantly with the increase of serum concentrations of total folate and 5-MTHF.
Parental knowledge and attitude of postoperative paediatric pain: stepwise linear regression analysis
Authors: Desalegn Mitiku , Shitemaw Tewoderos , Getahun Genanew Kassie , Lemma Lire 
Published: March 2024

BackgroundDespite the fact that mothers care for their children's pain in most cases, it has been noted that mothers have limited knowledge and attitude about paediatric pain. This study aims to assess parental knowledge and attitude of postoperative paediatric pain (POPP).MethodThis is institutional based cross sectional study conducted with 102 parents at Nigist Eleni Mohamed Memorial Comprehensive Specialized Hospital (NEMMCSH). A convenience sampling technique was used to select parents. This study has used a questionnaire (Parental Pain Expression Perception (PPEM), examine parents' attitudes and knowledge about how their children exhibit their pain and Medication Attitude Questioner (MAQs), focuses on how parents feel about giving their child analgesic medication to alleviate post-operative pain). Descriptive statistics were utilized to analyse the parent's response and presented with frequency and percentage. Factor analysis to analyze factor structure and stepwise linear regression analysis to examine the impact of socio-demographic factors in predicting parental knowledge and attitude about POPP were done. The statistical tests were performed at 95% confidence interval and 5% significance level.ResultA total of 102 parents fulfilling the inclusion criteria were included. About 78% of parents agreed that children always express pain by crying or whining. The majority of parents (75.6%) believe children who are playing are not in pain. Regarding parental attitudes about pain medications, majority of parents (61%) believe that children should be given pain medication as little as possible because of its side effects. According to about 26.8% of parents, giving children pain medication for pain might teach them to use drugs for other issues. On the other hand, 63.4% of parents say that giving children pain medication as little as possible is the most effective way to manage their pain. Parents of younger children and parents from rural area are more likely to score higher in attention seeking sub-score of PPEP while parents from urban residence and those parents who are employed are more likely to perceive about the side effects of pain medications (Side effects factors).ConclusionThe overall knowledge and attitude of parents about postoperative pain and pain medications were poor.
Sex differences in normal fetal regional brain apparent diffusion coefficient changes assessed by in utero DWI
Authors: Ren Jing-Ya , Zhu Ming , Dong Su-Zhen 
Published: March 2024

ObjectiveThere are differences in the vulnerability of male and female fetal brains to adverse intrauterine exposure, preterm birth, and associated perinatal brain injury. The main objective of this study was to identify any statistically significant difference in the change of apparent diffusion coefficient (ADC) in the intracranial regions of male and female fetuses in the second and third trimesters.MethodsDiffusion-weighted imaging (DWI) was performed in 200 fetuses between 20 and 37 gestational ages (GA) with normal results or suspicious results on sonography followed by structural MRI. Pairwise ADC values of the regions of interest (ROIs) were manually delineated on either side of the cerebral white matter: frontal white matter (FWM), parietal white matter (PWM), occipital white matter (OWM), temporal white matter (TWM), basal ganglia (BG), thalamus (THA), cerebellar hemisphere (CBM), and a single measurement in the pons. The changes in these values were studied over the gestational range, along with potential sex differences and asymmetries of the cerebral hemispheres.ResultsDuring the third trimester, ADC values in OWM, TWM, and CBM were significantly higher in male fetuses than those in female fetuses (p &lt; 0.05). After the correction of false-discovery rates (FDR), the difference in CBM was the only statistically significant (p = 0.0032). However, the decreased rate of ADC values in male fetuses in CWM (except for FWM), BG, THA, CBM, and pons was higher than that in female fetuses during the second and third trimesters.ConclusionsWe have shown some differences in the intracranial regional ADC changes between male and female fetuses using in utero DWI during the second and third trimesters.
Whole-transcriptome profiling and identification of cold tolerance-related ceRNA networks in japonica rice varieties
Authors: Wang Hao , Jia Yan , Bai Xu , Wang Jin , Liu Ge , Wang Haixing , Wu Yulong , Xin Junying , Ma Huimiao , Liu Zhenyu , Zou Detang , Zhao Hongwei 
Published: March 2024

IntroductionLow-temperature stress negatively impacts rice yield, posing a significant risk to food security. While previous studies have explored the physiological and linear gene expression alterations in rice under low-temperature conditions, the changes in competing endogenous RNA (ceRNA) networks remain largely unexamined.MethodsWe conducted RNA sequencing on two japonica rice varieties with differing cold-tolerance capabilities to establish ceRNA networks. This enabled us to investigate the transcriptional regulatory network and molecular mechanisms that rice employs in response to low-temperature stress.ResultsWe identified 364 differentially expressed circular RNAs (circRNAs), 224 differentially expressed microRNAs (miRNAs), and 12,183 differentially expressed messenger RNAs (mRNAs). WRKY family was the most prominent transcription factor family involved in cold tolerance. Based on the expression patterns and targeted relationships of these differentially expressed RNAs, we discerned five potential ceRNA networks related to low-temperature stress in rice: osa-miR166j-5p from the miR166 family was associated with cold tolerance; osa-miR528-3p and osa-miR156j-3p were linked to stress response; and osa-miR156j-3p was involved in the antioxidant system. In addition, Os03g0152000 in the antioxidant system, as well as Os12g0491800 and Os05g0381400, correlated with the corresponding stress response and circRNAs in the network. A gene sequence difference analysis and phenotypic validation of Os11g0685700 (OsWRKY61) within the WRKY family suggested its potential role in regulating cold tolerance in rice.Discussion and conclusionWe identified Os11g0685700 (OsWRKY61) as a promising candidate gene for enhancing cold tolerance in japonica rice. The candidate miRNAs, mRNAs, and circRNAs uncovered in this study are valuable targets for researchers and breeders. Our findings will facilitate the development of cold-tolerant rice varieties from multiple angles and provide critical directions for future research into the functions of cold-tolerance-related miRNAs, mRNAs, and circRNAs in rice.