bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telomerization of 3,3,3-Trifluoroprop-1-ene and Functionalization of Its Telomers
Authors: Kostov George K. , Améduri Bruno , Brandstadter Stephan 
Published: December 2008

The synthesis of four 3,3,3-trifluoroprop-1-ene telomers (RF(C3H3F3)nI, n = 1, 2, RF = n-C6F13- or (CF3)2CF-) and their allyl derivatives RF(C3H3F3)nCH2CH=CH2 are presented. The allyl telomers were prepared by a three-step reaction. The first step involved the thermal and peroxide-induced bulk telomerization of 3,3,3-trifluoroprop-1-ene (TFP) with heptafluoro-2-iodopropane or tridecafluoro-6-iodohexane leading to monoadduct and diadduct, the ratio of which depends on the R0 = [RFI]0/[TFP]0 initial molar ratio and the reaction temperature. The amount of monoadduct increased up to 50-60% and of the diadduct to 25-30% at temperatures up to 180 °C (thermal-initiated) and 150 °C (initiated with di-tert-butyl peroxide, DTBP), R0 up to 1.5. It was observed that the addition of the (CF3)2CF• radical onto the =CH2 of TFP was regioselective leading to selective formation of a single isomer in contrast to the addition of the n-C6F13• radical. Then, the telomers reacted with allyl acetate yielding RF(C3H3F3)nCH2CH(I)CH2OCOCH3 (n = 1, 2) in 50-80% yields. The third step consisted of a deiododeacetatization of these iodoacetates into RF(C3H3F3)nCH2-CH=CH2 (C,n) giving 50-80% yields. All the intermediates were characterized by 1H, 19F and products by 13C NMR spectroscopy.
Differences in left ventricular mass between overweight and normal-weight preadolescent children
Authors: Peralta-Huertas Jose , Livingstone Kristina , Banach Alayna , Klentrou Panagiota , O’Leary Deborah 
Published: December 2008

This study examined cardiac and arterial differences between overweight and normal-weight preadolescent children. Twenty children (10.2 ± 0.4 years of age) classified as overweight, on the basis of age-appropriate body mass index (BMI) cutoffs, were compared with 43 normal-weight controls. Height, mass, and body surface area were measured. Relative body fat and lean body mass were estimated from skinfold thickness. Each child’s weekly physical activity metabolic equivalent (PAME) was calculated using a standardized questionnaire, and his or her sexual maturation was self-assessed using the Tanner scale. Peak aerobic power was assessed using a cycle ergometer and normalized to lean body mass. Mean arterial pressure was calculated from systolic and diastolic blood pressure (DBP) measurements taken with a Finapres. Cardiac dimensions were measured, using Μ-mode 2-dimensional echocardiography, and normalized to body surface area and height2.7. Left carotid artery pulse pressure (CaPP) was assessed with applanation tomometry. Overweight boys and girls had a higher left ventricular mass (LVM) and LVMHT2.7 than normal-weight boys and girls. CaPP was signficantly lower in the overweight than in the normal-weight groups, whereas PAME and relative peak aerobic power were significantly higher in the boys than the girls. Although overweight children had significantly higher stroke volumes and cardiac outputs than normal-weight children, ejection fraction was similar in the weight groups. Adjusted LVMHT2.7 was associated with cardiac volume measurements, BMI, and DBP in normal-weight children, whereas in the overweight children LVMHT2.7 did not significantly correlate with any variable. In conclusion, we found that cardiovascular adaptations can be seen in prepubescent overweight children as young as 10 years of age.
Short-term high-intensity interval training improves phosphocreatine recovery kinetics following moderate-intensity exercise in humans
Authors: Forbes Sean C. , Slade Jill M. , Meyer Ronald A. 
Published: December 2008

Previous studies have shown that high-intensity training improves biochemical markers of oxidative potential in skeletal muscle within a 2-week period. The purpose of this study was to examine the effect of short-term high-intensity interval training on the time constant (τ) of phosphocreatine (PCr) recovery following moderate-intensity exercise, an in vivo measure of functional oxidative capacity. Seven healthy active subjects (age, 21 ± 4 years; body mass, 69 ± 11 kg) performed 6 sessions of 4–6 maximal-effort 30 s cycling intervals within a 2-week period, and 7 subjects (age, 24 ± 5 years; body mass, 80 ± 15 kg) served as controls. Prior to and following training, phosphorous-31 magnetic resonance spectroscopy (31P-MRS; GE 3T Excite System) was used to measure relative changes in high-energy phosphates and intracellular pH of the quadriceps muscles during gated dynamic leg-extension exercise (3 cycles of 90 s exercise and 5 min of rest). A monoexponential model was used to estimate the τ of PCr recovery. The τ of PCr recovery after leg-extension exercise was reduced by 14% with high-intensity interval training (pretraining, 43 ± 14 s vs. post-training, 37 ± 15 s; p < 0.05) with no change in the control group (44 ± 12 s vs. 43 ± 12 s, respectively; p > 0.05). These findings demonstrate that short-term high-intensity interval training is an effective means of increasing functional oxidative capacity in skeletal muscle.
Greater bilateral deficit in leg press than in handgrip exercise might be linked to differences in postural stability requirements
Authors: Magnus Charlene R.A. , Farthing Jonathan P. 
Published: December 2008

Bilateral deficit is defined as the difference in the summed force between contracting muscles alone and contracting contralateral homologous muscles in combination. The purpose of the study was to investigate how postural stability influences bilateral deficit by comparing an exercise requiring more postural stability (the leg press) with an exercise requiring less postural stability (the handgrip). Eight participants volunteered for the study (3 males, 5 females). Maximal strength was determined by a 1-repetition maximum for the leg press (weight machine) and handgrip (dynamometer) exercises. Electromyography was used to measure activation of the effectors (flexor carpi ulnaris for the handgrip and vastus lateralis for the leg press) and the core muscles (rectus abdominis and external obliques). Bilateral deficit was greater in the leg press (–12.08 ± 10.22%) than the handgrip (–0.677 ± 5.00%; p < 0.05). Muscle activation of the effectors and core muscles was not significantly different between unilateral and bilateral conditions for either exercise. However, core muscle activation was significantly greater during the leg press (48.30 ± 19.60 µV) than during the handgrip (16.50 ± 8.10 µV; p < 0.05) exercise. These results support the hypothesis that an exercise requiring more postural stability (e.g., the leg press) will have a larger deficit and greater activation of core muscles than an exercise requiring less postural stability (e.g., the handgrip). Since the bilateral deficit was only apparent for the leg press exercise, we conclude that postural stability requirements might influence the magnitude of bilateral deficit.
Caffeine and sports performance
Authors: Burke Louise M. 
Published: December 2008

Athletes are among the groups of people who are interested in the effects of caffeine on endurance and exercise capacity. Although many studies have investigated the effect of caffeine ingestion on exercise, not all are suited to draw conclusions regarding caffeine and sports performance. Characteristics of studies that can better explore the issues of athletes include the use of well-trained subjects, conditions that reflect actual practices in sport, and exercise protocols that simulate real-life events. There is a scarcity of field-based studies and investigations involving elite performers. Researchers are encouraged to use statistical analyses that consider the magnitude of changes, and to establish whether these are meaningful to the outcome of sport. The available literature that follows such guidelines suggests that performance benefits can be seen with moderate amounts (~3 mg·kg–1 body mass) of caffeine. Furthermore, these benefits are likely to occur across a range of sports, including endurance events, stop-and-go events (e.g., team and racquet sports), and sports involving sustained high-intensity activity lasting from 1–60 min (e.g., swimming, rowing, and middle and distance running races). The direct effects on single events involving strength and power, such as lifts, throws, and sprints, are unclear. Further studies are needed to better elucidate the range of protocols (timing and amount of doses) that produce benefits and the range of sports to which these may apply. Individual responses, the politics of sport, and the effects of caffeine on other goals, such as sleep, hydration, and refuelling, also need to be considered.
Thermotolerance induced at a fever temperature of 40 °C protects cells against hyperthermia-induced apoptosis mediated by death receptor signalling
Authors: Bettaieb Ahmed , Averill-Bates Diana A. 
Published: December 2008

Mild temperatures such as 40 °C are physiological and occur during fevers. This study determines whether mild thermotolerance induced at 40 °C can protect HeLa cells against activation of the death receptor pathway of apoptosis by lethal hyperthermia (42–45 °C). Protein expression of heat shock proteins (Hsps) 27, 32, 60, 72, 90, and 110 was increased in thermotolerant cells (3 h, 40°C). Lethal hyperthermia (42–43 °C) caused cell death by apoptosis, but at 45 °C there was a switch to necrosis. Mild thermotolerance protected cells against heat-induced apoptosis (Annexin V labelling). Hyperthermia induced apoptosis through generation of reactive oxygen species (ROS) and death receptor signalling. The antioxidant polyethylene glycol-catalase abrogated increased expression of Fas death ligand and caspase-8 activation in response to lethal hyperthermia (42–43 °C). Mild thermotolerance attenuated the heat induction of ROS and FasL, which were initiating events in death receptor activation and signalling. Mild thermotolerance inhibited early events in hyperthermia-induced death receptor apoptosis such as Fas-associated death domain (FADD) translocation to membranes, caspase-8 activation, and tBid translocation to mitochondria. Downstream events in apoptosis such as caspase-3 activation, cleavage of PARP and ICAD, and chromatin condensation were also diminished in thermotolerant cells. It is important to improve knowledge about adaptive responses induced by exposure to mild stresses, such as fever temperatures, which can protect cells against subsequent exposure to lethal stress.
Meal-induced insulin sensitization and its parasympathetic regulation in humans
Authors: Patarrão Rita S. , Lautt W. Wayne , Afonso Ricardo A. , Ribeiro Rogério T. , Guarino Maria P. , Fernandes Ana B. , Boavida José M. , Macedo M. Paula 
Published: December 2008

In animal studies, the whole-body glucose disposal effect of insulin is low in the fasted state or after atropine infusion, but doubles after a meal, consistent with the hepatic insulin-sensitizing substance (HISS) hypothesis. We tested how a standardized test meal and atropine affected the dynamic response to insulin in humans. Insulin sensitivity was assessed in healthy male subjects (aged 28.9 ± 1.9 years, body mass index 23.3 ± 0.8 kg·m–2) by using the rapid insulin sensitivity test (RIST), which is a transient euglycemic clamp. After a 24-hour fasting period, dynamic insulin sensitivity was assessed and then repeated 100 min after the test meal. In a second protocol, the volunteers were fed the standardized test meal and intravenous atropine (0.5 mg) or saline (control group) was administered 50 min before insulin sensitivity assessment. Insulin sensitivity increased in the fed state (232.1% ± 46.3%, n = 7) in comparison with the 24-hour fasted state. In the atropine protocol, the drug partially blocked (56.5% ± 11.6%, n = 6) insulin sensitivity. In humans, feeding resulted in increased insulin sensitivity. The low dose of atropine in humans lead to a partial HISS-dependent decrease in insulin sensitivity. Meal-induced insulin sensitization occured in humans by a similar mechanism as that reported in other species. The sensitization process was regulated by a cholinergic ‘feeding signal.’
Behavioral responses of white-tailed deer subjected to lethal management
Authors: Williams Scott C. , DeNicola Anthony J. , Ortega Isaac M. 
Published: December 2008

Currently, the most effective and cost-efficient mechanism for controlling overabundant white-tailed deer ( Odocoileus virginianus (Zimmermann, 1780)) is lethal removal, most commonly controlled hunting and sharpshooting. Deer subjected to such efforts may behave differently during removal and remaining deer may alter behaviors, potentially limiting future efficacy of removal efforts. Our objectives were to quantify changes in deer distribution in response to controlled hunting and sharpshooting. We immobilized two sample populations of 20 deer (one enclosed and one free-ranging) in central New Jersey, USA, and fitted them with global positioning system collars. The free-ranging herd experienced 11 days of controlled hunting, reducing density from 78 to 27 deer/km2. We subjected the enclosed herd to a 7 day sharpshoot, reducing density from 83 to 7 deer/km2. Hunted deer increased mean home ranges during removal, while deer exposed to sharpshooting did not. Collared doe–doe home-range overlap increased postsharpshoot, suggesting increased social interaction. Behaviors of hunted deer were directly affected by the human threat, while behavioral changes of deer exposed to sharpshooting were linked to population reduction. In the absence of an intact matrilineal social group, unrelated does will seek each other out in what appears to be an inherent need to be social.
Determinants of seasonal variation in activity patterns of mouflon
Authors: Bourgoin G. , Garel M. , Van Moorter B. , Dubray D. , Maillard D. , Marty E. , Gaillard J.-M. 
Published: December 2008

Balancing energetic requirements is a complex process governed by the current condition of the animal, its physiological state, and by the local environment. In response to these factors, animals are expected to adapt their activity patterns. Based on continuous monitoring of 14 GPS-collared animals in southern France, we studied how environmental conditions and reproductive life-cycle periods shape the monthly activity patterns of free-ranging female mouflon ( Ovis gmelini musimon × Ovis sp. (Blyth, 1841)). More specifically, we analyzed total daily activity, diurnal activity, length of activity bouts, and number of activity bouts. We performed multivariate analyses to account for the relationship between several intercorrelated explanatory variables and monthly activity variables. Mouflon decreased their daily activity, diurnal activity, and length of activity bouts during the hot summer months. Such changes might allow animals to reduce thermoregulation costs. The resulting higher activity in winter compared with summer indicates that summer is the most limiting period for large herbivores in Mediterranean area. Both the number of active bouts and the daily activity increased during spring, when females have access to increased food quality, which ensures a faster digestion, and when they may face high energetic requirements owing to reproduction.
COMPARISON OF TRANSIENT BALLISTIC ELECTRON TRANSPORT IN BULK WURTZITE PHASE 6H-SiC and GaN
Authors: ARABSHAHI H. 
Published: December 2008

An ensemble Monte Carlo simulation is used to compare bulk electron ballistic transport in 6H - SiC and GaN materials. Electronic states within the conduction band valleys at Γ1, U, M, Γ3, and K are represented by nonparabolic ellipsoidal valleys centered on important symmetry points of the Brillouin zone. The large optical phonon energy (~120 meV) and the large intervalley energy separation between the Γ and satellite conduction band valleys suggest an increasing role for ballistic electron effects in 6H - SiC , especially when compared with most III-V semiconductors such as GaAs . Transient velocity overshoot has been simulated, with the sudden application of fields up to ~5×107 Vm -1, appropriate to the gate-drain fields expected within an operational field effect transistor. A peak-saturation drift velocity ratio of 2:1 is predicted for 6H - SiC material while that for GaN is 4:1. The electron drift velocity relaxes to the saturation value of ~2×105 ms -1 within 3 ps, for both crystal structures. The transient velocity overshoot characteristics are in fair agreement with other recent calculations.
Breast Cancer–Associated Fibroblasts Confer AKT1-Mediated Epigenetic Silencing of Cystatin M in Epithelial Cells
Authors: Lin Huey-Jen L. , Zuo Tao , Lin Ching-Hung , Kuo Chieh Ti , Liyanarachchi Sandya , Sun Shuying , Shen Rulong , Deatherage Daniel E. , Potter Dustin , Asamoto Lisa , Lin Shili , Yan Pearlly S. , Cheng Ann-Lii , Ostrowski Michael C. , Huang Tim H.-M. 
Published: December 2008

Abstract
The interplay between histone modifications and promoter hypermethylation provides a causative explanation for epigenetic gene silencing in cancer. Less is known about the upstream initiators that direct this process. Here, we report that the Cystatin M (CST6) tumor suppressor gene is concurrently down-regulated with other loci in breast epithelial cells cocultured with cancer-associated fibroblasts (CAF). Promoter hypermethylation of CST6 is associated with aberrant AKT1 activation in epithelial cells, as well as the disabled INNP4B regulator resulting from the suppression by CAFs. Repressive chromatin, marked by trimethyl-H3K27 and dimethyl-H3K9, and de novo DNA methylation is established at the promoter. The findings suggest that microenvironmental stimuli are triggers in this epigenetic cascade, leading to the long-term silencing of CST6 in breast tumors. Our present findings implicate a causal mechanism defining how tumor stromal fibroblasts support neoplastic progression by manipulating the epigenome of mammary epithelial cells. The result also highlights the importance of direct cell-cell contact between epithelial cells and the surrounding fibroblasts that confer this epigenetic perturbation. Because this two-way interaction is anticipated, the described coculture system can be used to determine the effect of epithelial factors on fibroblasts in future studies. [Cancer Res 2008;68(24):10257–66]
Altered Detoxification Status and Increased Resistance to Oxidative Stress by K-Ras Transformation
Authors: Recktenwald Christian V. , Kellner Roland , Lichtenfels Rudolf , Seliger Barbara 
Published: December 2008

Abstract
Mutated K-ras is frequently found in human malignancies and plays a key role in many signal transduction processes resulting in an altered gene and/or protein expression pattern. Proteins controlled by a constitutive activated mitogen-activated protein kinase pathway are primarily related to alterations in the mitochondrial and nuclear compartments. Therefore, different K-Ras mutants and respective control cells were subjected to two-dimensional gel electrophoresis using basic pH gradients. This approach led to the identification of differentially expressed proteins, such as members of the heterogeneous ribonucleoprotein family, and enzymes involved in cellular detoxification as well as in oxidative stress. Increased expression of these enzymes was paralleled by an elevated tolerance of K-ras mutants against the cytotoxic potential of hydrogen peroxide and formaldehyde as well as an altered redox status based on enhanced intracellular glutathione (GSH) levels indicating an improved detoxification potential of defined K-ras transfectants, whereas down-regulation by RNA interference of candidate proteins reversed the tolerance against these compounds. This hypothesis is supported by an up-regulated expression of a key enzyme of the pentose phosphate pathway resulting in an increased production of NADPH required for anabolic processes as well as the rebuilding of oxidized GSH. Both the enhanced resistance against xenobiotic compounds as well as an altered oxidative pathway might confer growth advantages for tumor cells carrying dominant-positive K-ras mutations such as in lung or pancreatic adenocarcinoma. [Cancer Res 2008;68(24):10086–93]
Common Transcriptional Signature of Tumor-Infiltrating Mononuclear Inflammatory Cells and Peripheral Blood Mononuclear Cells in Hepatocellular Carcinoma Patients
Authors: Sakai Yoshio , Honda Masao , Fujinaga Haruo , Tatsumi Isamu , Mizukoshi Eishiro , Nakamoto Yasunari , Kaneko Shuichi 
Published: December 2008

AbstractHepatocellular carcinoma (HCC) is frequently associated with infiltrating mononuclear inflammatory cells. We performed laser capture microdissection of HCC-infiltrating and noncancerous liver-infiltrating mononuclear inflammatory cells in patients with chronic hepatitis C (CH-C) and examined gene expression profiles. HCC-infiltrating mononuclear inflammatory cells had an expression profile distinct from noncancerous liver-infiltrating mononuclear inflammatory cells; they differed with regard to genes involved in biological processes, such as antigen presentation, ubiquitin-proteasomal proteolysis, and responses to hypoxia and oxidative stress. Immunohistochemical analysis and gene expression databases suggested that the up-regulated genes involved macrophages and Th1 and Th2 CD4 cells. We next examined the gene expression profile of peripheral blood mononuclear cells (PBMC) obtained from CH-C patients with or without HCC. The expression profiles of PBMCs from patients with HCC differed significantly from those of patients without HCC (P &lt; 0.0005). Many of the up-regulated genes in HCC-infiltrating mononuclear inflammatory cells were also differentially expressed by PBMCs of HCC patients. Analysis of the commonly up-regulated or down-regulated genes in HCC-infiltrating mononuclear inflammatory cells and PBMCs of HCC patients showed networks of nucleophosmin, SMAD3, and proliferating cell nuclear antigen that are involved with redox status, the cell cycle, and the proteasome system, along with immunologic genes, suggesting regulation of anticancer immunity. Thus, exploring the gene expression profile of PBMCs may be a surrogate approach for the assessment of local HCC-infiltrating mononuclear inflammatory cells. [Cancer Res 2008;68(24):10267–79]