bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical utility of the Oncomine Dx Target Testmulti‐CDxsystem and the possibility of utilizing those original sequence data
Authors: Saito Ayaka , Terai Hideki , Kim Tae‐Jung , Emoto Katsura , Kawano Ryutaro , Nakamura Kohei , Hayashi Hideyuki , Takaoka Hatsuyo , Ogata Akihiko , Kinoshita Katsuhito , Ito Fumimaro , Shigematsu Lisa , Okada Masahiko , Fukushima Takahiro , Mitsuishi Akifumi , Shinozaki Taro , Ohgino Keiko , Ikemura Shinnosuke , Yasuda Hiroyuki , Kawada Ichiro , Soejima Kenzo , Nishihara Hiroshi , Fukunaga Koichi 
Published: March 2024

AbstractBackgroundCompanion diagnostic tests play a crucial role in guiding treatment decisions for patients with non‐small cell lung cancer (NSCLC). The Oncomine Dx Target Test (ODxTT) Multi‐CDx System has emerged as a prominent companion diagnostic method. However, its efficacy in detecting driver gene mutations, particularly rare mutations, warrants investigation.AimsThis study aimed to assess the performance of the ODxTT in detecting driver gene mutations in NSCLC patients. Specifically, we aimed to evaluate its sensitivity in detecting epidermal growth factor receptor (EGFR) mutations, a key determinant of treatment selection in NSCLC.Materials and MethodsWe conducted a retrospective analysis of NSCLC patients who underwent testing with the ODxTT at Keio University Hospital between May 2020 and March 2022. Patient samples were subjected to both DNA and RNA tests. Driver gene mutation status was assessed, and instances of missed mutations were meticulously examined.ResultsOf the 90 patients, five had nucleic acid quality problems, while 85 underwent both DNA and RNA tests. Driver gene mutations were detected in 56/90 (62.2%) patients. Of the 34 patient specimens, driver mutations were not detected using the ODxTT; however, epidermal growth factor receptor (EGFR) mutations were detected using polymerase chain reaction‐based testing in two patients, and aKRASmutation was detected by careful examination of the sequence data obtained using the ODxTT in one patient. For the above three cases, carefully examining the gene sequence information obtained using the ODxTT could identify driver mutations that were not mentioned in the returned test results. Additionally, we confirmed comparable instances of overlook results forEGFRmutations in the dataset from South Korea, implying that this type of oversight could occur in other countries using the ODxTT.EGFRmutation was missed in ODxTT in Japan (6.3%, 2/32), South Korea (2.0%, 1/49), and overall (3.7%, 3/81).ConclusionEven if sufficient tumor samples are obtained, rareEGFRmutations (which are excluded from the ODxTT's genetic mutation list) might not be detected using the current ODxTT system due to the program used for sequence analysis. However, such rareEGFRmutations can still be accurately detected on ODxTT's sequence data using next‐generation sequencing.
East Asian patients who received immunotherapy‐based therapy associated with improved survival benefit in advanced non‐small cell lung cancer: An updated meta‐analysis
Authors: Yao Yueyuan , Li Butuo , Xu Yiyue , Yang Linlin , Zou Bing , Wang Linlin 
Published: March 2024

AbstractBackgroundImmune checkpoint inhibitors (ICIs) combined with chemotherapy have been recommended as the standard treatment for advanced NSCLC patients without driver‐gene mutations. However, there are different genetic characteristics and biological traits of tumors between non‐East Asian (nEA) and East Asian (EA) patients with NSCLC, which may contribute to differences in the efficacy of ICIs in different ethnic populations. Previous findings regarding differences in the efficacy of ICIs among ethnic groups have been inconsistent. Therefore, we performed a meta‐analysis by collecting published data to investigate the clinical outcomes of ICIs for EA NSCLC patients compared to nEA patients.MethodsOverall survival (OS) and progression‐free survival (PFS) were used to access the difference in survival outcomes between the two populations. Subgroup analyses were performed based on the line of ICIs, the use of ICIs alone or in combination, and the type of ICIs.ResultsA total of 9826 NSCLC patients from 21 randomized controlled trials (RCTs) with 4064 EAs were included, which involved PD‐1, PD‐L1, and CTLA‐4 inhibitors. EA NSCLC patients who received ICIs‐based therapy were associated with significantly improved survival benefits in OS (p = 0.02) compared with nEA patients. Subgroup analysis indicated that EA patients receiving first‐line ICIs showed significantly superior OS compared with nEA patients (p = 0.007). Chemo‐ICIs treatment showed significant advantages in terms of OS (p = 0.002) and PFS (p = 0.02) among EA patients compared to nEA patients. In addition, PD‐1 inhibitors were associated with improved OS among both EA patients and nEA patients compared with PD‐L1 inhibitors.ConclusionEA NSCLC patients who received ICIs‐based therapy were associated with significantly improved survival benefits compared with nEA NSCLC patients. Earlier intervention with ICIs and combination treatment was more recommended for EA NSCLC patients. Moreover, PD‐1 inhibitors are associated with prolonged survival among both EA and nEA patients.
Exploring the Anti‐Cancer Potential of Hispidin: A Comprehensive in Silico and in Vitro Study on Human Osteosarcoma Saos2 Cells
Authors: Benarous Khedidja , Serseg Talia , Mermer Arif , Tahmasebifar Aydin , Boulebd Houssem , Linani Abderahmane 
Published: March 2024

AbstractHispidin was initially discovered in basidiomycete Inonotus hispidus (Bull.) P. Karst and this extraordinary compound possesses immense potency and can be extracted from the wild mushroom through specialized bioreactor cultivation techniques. In our study, we isolated it from Inonotus hispidus (Bull.) P. Karst., with a yield of 3.6 %. We identified and characterized hispidin through the implementation of spectroscopic techniques such as FTIR, NMR, and MS. Additionally, we utilized Thermogravimetric Analysis for thermal characterization of the compound. Computational studies based on DFT were performed to investigate the molecular structure, electronic properties, and chemical reactivity of hispidin. PASS analysis for hispidin demonstrated that 19 of them are anti‐neoplastic activities. The Pharmacology prediction of hispidin confirm that it is not toxic, non‐carcinogenesis with a good human intestinal absorption. The effect of hispidin on the viability of bone cancer cells was evaluated by MTT assay. The results showed that hispidin significantly reduced SaoS2 cell viability in a dose‐dependent manner. Molecular docking was carried out using five targets related to bone cancer to determine the interactions between hispidin and the studied proteins. The results demonstrate that hispidin is a good inhibitor for the five targets. Dynamic simulation shows a good stability of the complex hispidin‐protein.
Design, Synthesis and In vitro Antitubercular Effect of New Chalcone Derivatives Coupled with 1,2,3‐Triazoles: A Computational Docking Techniques
Authors: Sadineni Kumaraswamy , Reddy Basireddy Sravanthi , Rao Allaka Tejeswara , Yatam Satyanarayana , Bhoomandla Srinu , Muvvala Venkatanaryana , Babu Haridasyam Sharath 
Published: March 2024

AbstractA very interesting foundation for this study is the creation of new methods for modifying compounds with a 1,2,3‐triazole and chalcone scaffolds, as these compounds are significant in organic synthesis, particularly in the synthesis of bioactive organic compounds. To contribute to the development of an efficient method for the conversion of antimicrobial and antituberculosis heterocyclics, a novel series of cyclohepta pyridinone fused 1,2,3‐triazolyl chalcones were designed and synthesized. All the newly prepared scaffolds were characterized by FT‐IR, NMR (1H & 13C) and mass spectrometry. Among the tested compounds, hybrids 8b, 8d, and 8f exhibited exceptional antibacterial susceptibilities with zone of inhibition 27.84±0.04, 32.27±0.02, and 38.26±0.01 mm against the tested E. faecalis bacteria, whereas 8d had better antitubercular potency against M. tuberculosis H37Rv strain with MIC value 5.25 μg/mL, compared to Streptomycin [MIC=5.01 μg/mL]. All the synthesized compounds were initially assessed in silico against the targeted protein i. e., DprE1 that indicated compound 8d, 8f and 8h along with several other 1,2,3‐triazole compounds as possible inhibitors. Based on docking results, 8d showed that the amino acids His74(A), Lys76(A), Cys332(A), Asp331(A), Val307(A), Tyr357(A), Met226(A), Gln276(A), Gly75(A), Peo58(A), Leu259(A), and Lys309(A) exhibited highly stable binding to DprE1 receptor of Mycobacterium tuberculosis (PDB: 4G3 U). Moreover, these scaffolds physicochemical characteristics, filtration molecular properties, assessment of toxicity, and bioactivity scores were assessed in relation to ADME (absorption, distribution, metabolism, and excretion).
OptoProfilin: A Single Component Biosensor of Applied Cellular Stress
Authors: Mann Noah , Hill Jahiem , Wang Kenneth , Hughes Robert M. 
Published: March 2024

AbstractThe actin cytoskeleton is a biosensor of cellular stress and a potential prognosticator of human disease. In particular, aberrant cytoskeletal structures such as stress granules formed in response to energetic and oxidative stress are closely linked to ageing, cancer, cardiovascular disease, and viral infection. Whether these cytoskeletal phenomena can be harnessed for the development of biosensors for cytoskeletal dysfunction and, by extension, disease progression, remains an open question. In this work, we describe the design and development of an optogenetic iteration of profilin, an actin monomer binding protein with critical functions in cytoskeletal dynamics. We demonstrate that this optically activated profilin (‘OptoProfilin’) can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines. Notably, OptoProfilin is a single component biosensor, likely increasing its utility for experimentalists. While a large body of preexisting work closely links profilin activity with cellular stress and neurodegenerative disease, this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress‐induced changes in the cytoskeleton.
Investigating the efficacy of peer tutoring to support Latinx students in Hispanic serving community colleges
Authors: Herrera Brooklyn Cole , Lanford Michael 
Published: March 2024

AbstractAlthough open‐access Hispanic Serving Institutions (HSIs) continue to expand across the United States, they are often under‐resourced and lack adequate institutional support services. This article presents findings from a qualitative case study of an asset‐based, peer support program for Latinx students in an open‐access HSI in the Southeast United States. The article's findings, informed by the theory of community cultural wealth, indicate that peer tutoring honors the linguistic capital of Latinx students, nurturesfamiliawithin the institution, enhances students’ navigational capital, and helps students navigate the nuances of social capital by encouraging the maintenance of their cultural integrity as they strengthen tutor‐tutee relationships and forge relationships across their institution. Therefore, this article argues that identity‐affirming peer support programs could efficiently and effectively ameliorate Latinx student challenges in under‐resourced MSIs, particularly at the community college level, thereby generating a more inclusive institutional environment conducive to student success.
Creating a culture of servingness for Latinx engineering students in Hispanic‐serving community colleges
Authors: Rodriguez Sarah L. , Berhane Bruk 
Published: March 2024

AbstractThis paper will describe the promise Hispanic‐serving Community Colleges (HSCCs) have for creating a culture of servingness, including curricular and co‐curricular supports, transfer infrastructure, engineering identity development, and other mechanisms that can positively impact Latinx engineering learners. We will outline current research and trends regarding Latinx engineering collegians at HSCCs. In addition, we will provide recommendations for how community college practitioners at HSCCs can promote a culture of servingness and encourage the development of Latinx engineering students.
Naphthalene diimide–Annulated Heterocyclic Acenes: Synthesis, Electrochemical and Semiconductor Properties and their Multifaceted Applications
Authors: Bhardwaj Abhishek , Mudasar Hussain Ch. , Dewangan Pratik , Mukhopadhyay Pritam 
Published: March 2024

AbstractAcenes and Naphthalene Diimides (NDIs) stand as distinguished classes of organic compounds, each possessing unique and intriguing properties that have garnered significant attention across various scientific disciplines. Acenes, characterized by linearly fused aromatic rings, have captivated researchers due to their diverse electronic structures and promising applications in materials science. On the other hand, NDIs, known for their distinctive electron–accepting properties, exhibit remarkable versatility in fields ranging from organic electronics, supramolecular to spin chemistry. In this review, we navigate through the fascinating realms of both acenes and NDIs before converging our focus on the highly diverse and distinctive subgroup of NDI–annulated heterocyclic acenes. This potentially important subgroup, has emerged as a subject of intense investigation, encapsulating their fascinating synthesis, optical and electrochemical characteristics, and multifaceted applications that span the realms of chemistry, physics, and biology. Through the exploration of their synthetic strategies, unique properties, and diverse applications, this review aims to offer a comprehensive understanding of the pivotal role played by NDI‐based heterocyclic acenes in contemporary multidisciplinary research and technological innovation.
Enhanced process monitoring for industrial coking furnace using a dual‐channel pooling and homologous bilinear model‐based convolutional neural network
Authors: Hua Chunle , Cui Yuancun , Wu Feng , Zhang Ridong 
Published: March 2024

AbstractThis paper introduces a process monitoring method using a convolutional neural network (CNN) with dual‐channel pooling and homologous bilinear models (DCP‐HBM). This method aims to enhance the stability of industrial coking furnaces and reduce maintenance costs. It is significantly relevant for addressing challenges such as complexity, high dimensionality, and strong coupling, which are difficult to manage with traditional fault diagnosis methods. In this study, a deep feature extraction module is employed to gather feature information. To effectively reduce the impact of noise and strengthen the correlation between features, DCP is incorporated after deep feature extraction. Concurrently, a HBM is introduced for feature fusion, further refining the characteristics of each state. Faults are then accurately classified through a classification module. The method utilizes DCP to filter and focus input data, thus making the model more attentive to task‐relevant information. This enhances the model's understanding and representation of the input data. The introduction of the HBM further refines the features extracted by the network and increases the precision of feature extraction. This leads to improved recognition of faults with high similarity, thereby enhancing the accuracy of the overall process monitoring. Experimental results demonstrate that this method exhibits strong performance in the monitoring of industrial coking furnaces, indicating its wide‐ranging application prospects.
Effects of pharmacological interventions on mortality in patients with Takotsubo syndrome: a report from the SWEDEHEART registry
Authors: Petursson Petur , Oštarijaš Eduard , Redfors Björn , Råmunddal Truls , Angerås Oskar , Völz Sebastian , Rawshani Araz , Hambraeus Kristina , Koul Sasha , Alfredsson Joakim , Hagström Henrik , Loghman Henareh , Hofmann Robin , Fröbert Ole , Jernberg Tomas , James Stefan , Erlinge David , Omerovic Elmir 
Published: March 2024

AbstractAimsTakotsubo syndrome (TS) is a heart condition mimicking acute myocardial infarction. TS is characterized by a sudden weakening of the heart muscle, usually triggered by physical or emotional stress. In this study, we aimed to investigate the effect of pharmacological interventions on short‐ and long‐term mortality in patients with TS.Methods and resultsWe analysed data from the SWEDEHEART (the Swedish Web System for Enhancement and Development of Evidence‐based care in Heart disease Evaluated According to Recommended Therapies) registry, which included patients who underwent coronary angiography between 2009 and 2016. In total, we identified 1724 patients with TS among 228 263 individuals in the registry. The average age was 66 ± 14 years, and 77% were female. Nearly half of the TS patients (49.4%) presented with non‐ST‐elevation acute coronary syndrome, and a quarter (25.9%) presented with ST‐elevation myocardial infarction. Most patients (79.1%) had non‐obstructive coronary artery disease on angiography, while 11.7% had a single‐vessel disease and 9.2% had a multivessel disease. All patients received at least one pharmacological intervention; most of them used beta‐blockers (77.8% orally and 8.3% intravenously) or antiplatelet agents [aspirin (66.7%) and P2Y12 inhibitors (43.6%)]. According to the Kaplan–Meier estimator, the probability of all‐cause mortality was 2.5% after 30 days and 16.6% after 6 years. The median follow‐up time was 877 days. Intravenous use of inotropes and diuretics was associated with increased 30 day mortality in TS [hazard ratio (HR) = 9.92 (P < 0.001) and HR = 3.22 (P = 0.001), respectively], while angiotensin‐converting enzyme inhibitors and statins were associated with decreased long‐term mortality [HR = 0.60 (P = 0.025) and HR = 0.62 (P = 0.040), respectively]. Unfractionated and low‐molecular‐weight heparins were associated with reduced 30 day mortality [HR = 0.63 (P = 0.01)]. Angiotensin receptor blockers, oral anticoagulants, P2Y12 antagonists, aspirin, and beta‐blockers did not statistically correlate with mortality.ConclusionsOur findings suggest that some medications commonly used to treat TS are associated with higher mortality, while others have lower mortality. These results could inform clinical decision‐making and improve patient outcomes in TS. Further research is warranted to validate these findings and to identify optimal pharmacological interventions for patients with TS.
Loop diuretic down‐titration at discharge in patients hospitalized for acute heart failure
Authors: Croset François , Llàcer Pau , Núñez Julio , Campos Jorge , García Marina , Pérez Alberto , Fernández Cristina , Fabregate Martín , López Genoveva , Tello Susana , Fernández José María , Ruiz Raúl , Manzano Luis 
Published: March 2024

AbstractAimsThe current literature provides limited guidance on the best diuretic strategy post‐hospitalization for acute heart failure (AHF). It is postulated that the efficacy and safety of the outpatient diuretic regimen may be significantly influenced by the degree of fluid overload (FO) encountered during hospitalization. We hypothesize that in patients with more pronounced FO, reducing their regular oral diuretic dosage might be associated with an elevated risk of unfavourable clinical outcomes.Methods and resultsIt was a retrospective observational study of 410 patients hospitalized for AHF in which the dose of furosemide at admission and discharge was collected. Patients were categorized across diuretic dose status into two groups: (i) the down‐titration group and (ii) the stable/up‐titration group. FO status was evaluated by a clinical congestion score and circulating biomarkers. The endpoint of interest was the composite of time to all‐cause death and/or heart failure readmission. A multivariable Cox proportional hazard regression model was constructed to analyse the endpoints. The median age was 86 (78–92) years, 256 (62%) were women, and 80% had heart failure with preserved ejection fraction. After multivariate adjustment, the down‐titration furosemide equivalent dose remained not associated with the risk of the combined endpoint in the whole sample (hazard ratio 1.34, 95% confidence interval 0.86–2.06, P = 0.184). The risk of the combination of death and/or worsening heart failure associated with the diuretic strategy at discharge was significantly influenced by FO status, including clinical congestion scores and circulating proxies of FO like BNP and cancer antigen 125.ConclusionsIn patients hospitalized for AHF, furosemide down‐titration does not imply an increased risk of mortality and/or heart failure readmission. However, FO status modifies the effect of down‐titration on the outcome. In patients with severe congestion or residual congestion at discharge, down‐titration was associated with an increased risk of mortality and/or heart failure readmission.
Capillary electrophoresis–mass spectrometry for creatinine analysis in residual clinical plasma samples and comparison with gold standard assay
Authors: van Mever Marlien , He Bingshu , van Veen Mariam , Slaats Jeroen , Buijs Madelon M. , Wieringa Joanne E. , Hankemeier Thomas , de Winter Peter , Ramautar Rawi 
Published: March 2024

AbstractWhen hospitalized, infants, particularly preterm, are often subjected to multiple painful needle procedures to collect sufficient blood for metabolic screening or diagnostic purposes using standard clinical tests. For example, at least 100 µL of whole blood is required to perform one creatinine plasma measurement with enzymatic colorimetric assays. As capillary electrophoresis–mass spectrometry (CE–MS) utilizing a sheathless porous tip interface only requires limited amounts of sample for in‐depth metabolic profiling studies, the aim of this work was to assess the utility of this method for the determination of creatinine in low amounts of plasma using residual blood samples from adults and infants. By using a starting amount of 5 µL of plasma and an injection volume of only 6.7 nL, a detection limit (S/N = 3) of 30 nM could be obtained for creatinine, and intra‐ and interday precisions (for peak area ratios) were below 3.2%. To shorten the electrophoretic separation time, a multi‐segment injection (MSI) strategy was employed to analyze up to seven samples in one electrophoretic run. The findings obtained by CE–MS for creatinine in pretreated plasma were compared with the values acquired by an enzymatic colorimetric assay typically used in clinical laboratories for this purpose. The comparison revealed that CE–MS could be used in a reliable way for the determination of creatinine in residual plasma samples from infants and adults. Nevertheless, to underscore the clinical efficacy of this method, a subsequent investigation employing an expanded pool of plasma samples is imperative. This will not only enhance the method's diagnostic utility but also contribute to minimizing both the amount and frequency of blood collection required for diagnostic purposes.
History, Life History, and Fate of a Salmonid Flagship Species: The Danube Salmon
Authors: Pinter Kurt , Ratschan Clemens , Unfer Günther , Weiss Steven , Jungwirth Mathias , Schmutz Stefan 
Published: March 2024

Abstract
The Danube Salmon Hucho hucho, endemic to the Danube River basin, is one of the largest members of the salmonid family. Here, we describe the distribution, ecology, and status of this flagship species in the upper Danube River basin. We model the historical distribution of Danube Salmon in the Austrian Danube River basin based on a review of the literature and species records. Fish sampling data over the past few decades illustrate the dramatic decline of the species and the population characteristics within the ecological niche that the species occupies. In conclusion, measures for the long-term conservation of Danube Salmon in the upper Danube River, as well as possibilities for sustainable management, are addressed and discussed.