bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibodies against human T‐cell leukemia/lymphoma virus (HTLV) and expression of HTLV p19 antigen in relatives of a T‐cell leukemia patient originating from Surinam
Authors: Vyth‐Dreese Florry A. , Rümke Philip , Robert‐Guroff Marjorie , De Lange Gerda , Gallo Robert C. 
Published: February 2007

AbstractSerum and peripheral blood cell samples from eleven relatives of a T‐cell leukemia patient with human T‐cell leukemia/lymphoma virus (HTLV)‐associated disease, were investigated for the presence of HTLV antibody and antigen expression. In addition to the patient, three family members were seropositive and a wide range in HTLV antibody titer was observed. The father of the patient showed the highest titer (173,000) and carried HTLV p19+ cells in his peripheral blood. Upon induction of proliferation of these cells by short‐term culture in the presence of phytohemagglutinin (PHA) and 12‐O‐tetradecanoyl phorbol‐13‐acetate (TPA), an increase from 1% to 28% HTLV p19+ cells was observed confirming previous findings that HTLV p19 expression was correlated with proliferative activity of the host cells. In none of the other seronegative or seropositive relatives of the patient HTLV p19 expression was revealed when tested in freshly isolated mononuclear cells, upon short‐term incubation in PHA + TPA or after prolonged culture for 2 or 3 passages in medium containing T‐cell growth factor. The results from HLA typing studies did not provide any evidence for HTLV related abnormalities in haplotype expression. Our data confirmed the earlier notion of a prevalence of HTLV infection within families of patients with HTLV‐associated disease. It is furthermore obvious from our results that a normal individual may possess high titer HTLV antibody and circulating HTLV p19+ cells without showing signs of disease.
Yolk‐sac carcinoma of extra‐embryonic origin in the 129 Sv/Sl mouse
Authors: Sobis Halina , Van Hove Luc , Vandeputte Michel 
Published: February 2007

AbstractDisplacement of the visceral yolk‐sac in fetectomized rats and hamsters does not only induce benign teratomas but may also lead to the development of malignant yolksac carcinoma. In the mouse, displacement of this extraembryonic membrane readily induces teratoma but until now yolk‐sac carcinoma has never been observed in this species. In the present study we report the development of yolk‐sac carcinoma in 129 Sv/Sl mice after a long latent period (8 months). The tumor is composed of visceral and parietal patterns in the primary neoplasm and in its metastases. It develops from the extra‐embryonal part belonging to normal (+/+), heterozygous (SI/+) or sterile (SI/SI) embryos, which proves that these yolk‐sac carcinomas are not of germ‐cell origin.
Expression of gamma‐glutamyltranspeptidase in cultures of spontaneously and chemically transformed rat liver cells
Authors: Miyazaki Masahiro , Wahid Syarifuddin , Miyano Keiko , Yabe Tamae , Sato Jiro 
Published: February 2007

AbstractThe expression of gamma‐glutamyltranspeptidase (gamma‐GTP) and the relationship between this enzyme activity and tumorigenicity were studied in 38 tumorigenic and non‐tumorigenic epithelial cell lines derived from livers of normal and aminoazo‐dye‐fed rats. Nine of 22 non‐tumorigenic epithelial cell lines exhibited gamma‐GTP‐positive cells, whereas 9 of 16 tumorigenic epithelial cell lines did not exhibit gamma‐GTP‐positive cells. This finding revealed that no correlation existed between the acquisition of gamma‐GTP activity and tumorigenicity. None of 10 spontaneously transformed epithelial cell lines, which consisted of 8 tumorigenic and 2 nontumorigenic cell lines, exhibited gamma‐GTP‐positive cells. On the other hand, 16 of 28 transformed epithelial cell lines, which were derived from aminoazo‐dye‐fed rat livers and aminoazo‐dye‐treated cultures of normal rat liver cells, included gamma‐GTP‐positive cells in various percentages. As described above, in contrast to in vitro spontaneous transformation of rat liver cells, it is obvious that the carcinogen‐induced transformation of rat liver cells in vitro as well as in vivo was frequently accompanied by the acquisition of gamma‐GTP‐activity. Therefore, it is concluded that the expression of gamma‐GTP in liver cells may be mainly associated with exposure to chemical carcinogens.
Precipitable immune complexes in Hodgkin's disease
Authors: Euler Hans‐Hartwig , Béress Rosemarie , Moldenhauer Imme , Löffler Helmut 
Published: February 2007

AbstractSera from 28 untreated patients with Hodgkin's disease and from 120 healthy controls were investigated for the presence of circulating immune complexes using a modified 3% polyethylene glycol precipitation method with subsequent quantification of the precipitated protein. Elevated levels of precipitable protein were found in 79% (p <0. 005) of Hodgkin's disease sera. The degree of elevation was associated with disease activity including the presence of B‐symptoms. Constant and pronounced increase of precipitable protein was found in six patients with stage‐III B nodular sclerosis subtype, thus exceeding the average amount of precipitable protein in healthy controls by a factor of 3–4. The erythrocyte sedimentation rate in 20 patients correlated with the amount of precipitable protein (r = 0. 79). Additionally, partial component analysis of the precipitates was carried out by laser nephelometry. Immunoglobulins and complement components were identified as being major components of the precipitated material in sera both from patients and healthy controls, thus confirming the probability of the immune complex nature of the precipitates. Significant differences between patients and healthy controls concerned the amount of precipitable components. Elevation of precipitable IgM was found to be the most sensitive parameter (86% above X̄ + 2 sd of normal controls, p <0. 005). Increased amounts of precipitable IgG, C4, and Clq were found in 57‐46% of patients' sera. Elevation of precipitable IgA and C3c were identified less often. The results suggest the quantification of precipitable immune complexes and their components to be of value as adjuncts in determining disease activity in Hodgkin's dis‐ease.
Oesophageal cancer in non‐smoking drinkers and in non‐drinking smokers
Authors: Tuyns Albert J. 
Published: February 2007

AbstractAlcohol and tobacco are the two major risk factors for cancer of the oesophagus; they usually combine their action. The action of each factor is studied here in non‐users of the other. Among non‐drinking male smokers the relative risk is higher than 5; among non‐smoking drinkers, risks are much higher and increase with average daily intake; this effect is also observed in females, with risk values of the same order of magnitude as for males. Thus the high sex ratio of oesophageal cancer in France can be entirely ascribed to different drinking levels in females and in males.
Preliminary identification of a tumor‐associated glycoprotein in bilharzial bladder cancer urine
Authors: Higashi Gene I. , El‐Asfahani Adel M. A. 
Published: February 2007

AbstractA tumor‐associated antigen was preliminarily identified in urine from bilharzial (squamous‐cell carcinoma) bladder cancer patients. Monospecific rabbit antisera were made by immunization with concentrated bladder cancer urine and exhaustive absorption with insoluble normal human serum and urine. The urine tumor‐associated antigen was identical to an antigen from 3M KCl bladder tumor extract by immunodiffusion. The antigen in urine was found in nine of 10 bladder cancer patients and was absent from normal urine and serum by immunodiffusion and immunoelectrophoresis. The antigen was a concanavalin‐A‐binding glycoprotein which was anodal on immunoelectrophoresis. It was stable up to 2 years at‐20°C and did not cross‐react with carcinoembryonik antigen or with Schistosoma haematobium antigens.
Analysis of spontaneous, carcinogen‐induced and promoter‐induced chromosomal instability in patients with hereditary retinoblastoma
Authors: Gainer H. St. C. , Kinsella A. R. 
Published: February 2007

AbstractSkin fibroblasts from patients with hereditary retino‐blastoma (RB cells) were examined since predisposition to the tumour might be expected to involve some degree of chromosomal instability, as has been noted for several cancer‐prone conditions. Spontaneous and N‐Methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG)‐induced aberration frequencies were measured, the cytogenetic effects of long‐term treatment with 12‐O‐tetradecanoyl‐phorbol‐13acetate (TPA) were examined and also the spontaneous and TPA‐induced sister chromatid exchange (SCE) frequencies. In all the studies the RB cells behaved in a similar fashion to normal human skin fibroblasts.
Double minutes in acute myeloid leukemia
Authors: Li You‐Sheng 
Published: February 2007

AbstractFive out of 110 patients with acute myeloid leukemia (AML) showed double minutes (DM) in cytogenetic preparations. DM were found to occur predominantly in elderly patients, and in erythroleukemia or acute myelomonocytic leukemia. The five patients were apparently divided into two groups according to the number of DM per cell. Patients with few sets of DM had exclusively complex karyotypes and a poor prognosis while patients with numerous DM had otherwise normal or nearly normal karyotypes and a good prognosis. Data on II previously reported cases of AML with DM were collected from the literature and consistent results were revealed except that several of the II patients were diagnosed as having acute myeloblastic leukemia (Ml and M2 in the FAB classification), which is apparently due to differences in diagnostic criteria. Therefore, numerous or few DM in a cell might relate to different entities. When the number of DM was small they might have resulted from breakdown of existing chromosomes.
Caffeine (1,3,7‐trimethylxanthine), a temperate promoter of DMBA‐induced rat mammary gland carcinogenesis
Authors: Welsch Clifford W. , Scieszka Karen M. , Senn Eric R. , Dehoog Jane V. 
Published: February 2007

AbstractThe administration of caffeine to the drinking water (250 mg/l and 500 mg/l) of female Sprague‐Dawley rats after treatment with 7,12‐dimethylbenzanthracene (DMBA) (5 mg i.g.) resulted in an increase in mammary carcinoma incidence. The confidence levels of statistical significance for the groups of rats receiving the 250‐mg and 500‐mg doses of caffeine were >0.70 and >0.99, respectively. This increase in mammary carcinoma incidence was observed when caffeine treatment was initiated commencing 3 days after DMBA treatment and continued for 21 weeks. This effect was also observed when caffeine treatment was initiated commencing 20 weeks after DMBA treatment and continued for 6 weeks in rats relatively refractory to carcinogen treatment (mammary tumor‐free at onset of treatment) and in rats relatively sensitive to the carcinogen (mammary tumor bearing at onset of caffeine treatment). Caffeine treatment of rats prior to and during carcinogen treatment did not significantly affect mammary carcinoma incidence. Thus caffeine consumption has been shown to significantly enhance the promoting phase but is without effect on the initiating phase of this carcinogenic process.
Murine non‐lymphoid tumors are lysed by a combination of NK and NC cells
Authors: Lattime Edmund C. , Pecoraro Gene A. , Cuttito Michael J. , Stutman Osias 
Published: February 2007

AbstractNatural cell‐mediated cytotoxicity (NCMC) against a variety of tumor targets is mediated by a heterogeneous group of effector cells with the natural killer (NK) and natural cytotoxic (NC) cells being the predominant prototypes in mice. This report shows that non‐lymphoid tumor targets, mostly derived from chemically induced fibrosarcomas, are susceptible to either (I) NK‐mediated lysis with all the activity being the function of a poly‐IC augmentable Qa‐5+ effector cell; (2) NC‐mediated lysis with all activity being the function of a Qa‐5—cell not augmented by poly‐IC; and (3) a combination of NK‐and NC‐mediated lysis with activity being the function of both Qa‐5+ and Qa‐5‐ cells, the NK (Qa‐5+) augmented by poly‐IC. These studies further support the view that murine NC and NK cells are distinct and collectively make up the NCMC system, and also that the previous association of NK cells with lymphoid tumor lysis and NC cells with nonlymphoid tumor lysis is not a valid one.
Human papillomavirus type‐16‐related DNA in genital Bowen's disease and in bowenoid papulosis
Authors: Ikenberg Hans , Gissmann Lutz , Gross Gerd , Grussendorf‐Conen Elke‐Ingrid , Hausen Harald Zur 
Published: February 2007

Abstract32P‐labelled DNA of HPV 16 which has been isolated and molecularly cloned from a cervical carcinoma (Dürst et al., 1983) was used to screen the cellular DNAs obtained from 20 different biopsies of Morbus Bowen or Bowenoid papulosis, respectively, by Southern blot analysis. Under conditions of differing stringency for the hybridization, HPV 16 DNA or related sequences were identified in 6 out of 10 cases of Morbus Bowen (4 out of 5 from a genital localization) and in 8 out of 10 biopsies from Bowenoid papulosis. One additional case of the latter disease contained DNA sequences of an HPV type not yet classified. There is evidence for the presence of another HPV DNA in two of the HPV‐16‐positive tumors. A large number of normal genital tissue samples were negative for HPV DNA.
Artificial sweeteners and absence of bladder cancer risk in Copenhagen
Authors: Møller‐Jensen O. , Knudsen J. B. , Sørensen B. L. , Clemmesen J. 
Published: February 2007

AbstractDuring the years 1979 to 1981 a population‐based case‐control study of bladder cancer including papillomas was performed in Greater Copenhagen. After exclusions some 388 patients (290 males; 98 females) and an age‐and sex‐matched group of 787 controls (592 males; 195 females) remained for analysis. Controls were selected at random from the general population of the study area. All persons were interviewed concerning use of artificial sweeteners in addition to their exposure to a number of other known or suspected risk factors for bladder cancer. Fifty‐five male bladder cancer patients (19.4%) and 150 controls (25.7%) had at some time used artificial sweeteners regularly. Among females 27.1% of cases and 25.9% of controls regularly used sweeteners. In neither sex was the relative risk significantly increased in users compared with non‐users of artificial sweeteners. The relative risk of 0.78 in the two sexes combined was not significantly different from 1.0 (95% C.I.: 0.58‐1.05). There was no indication of a regular increase in risk with increasing daily consumption of table‐top sweeteners nor was there any indication of an increase in risk with a duration of regular use of artificial sweeteners. Taking into account a possible latency period between first regular use and bladder cancer development did not change the finding of an absence of association between use of artificial sweeteners and the risk of bladder cancer. Neither saccharine nor cyclamate users had an increased risk of bladder cancer. This population‐based case‐control investigation provides further evidence that it is highly unlikely that the consumption of artificial sweeteners has contributed to current bladder cancer rates in man.
Changes in lipid profiles of estrogen‐induced and transplanted renal carcinomas in Syrian hamsters
Authors: Talley Deanna J. , Sadowski James A. , Boler Sarah A. , Li Jonathan J. 
Published: February 2007

AbstractThe lipid profiles of estrogen‐induced primary renal carcinomas and hormone‐dependent and ‐independent transplanted tumors were compared with those of both normal hamster kidneys and renal tissues of tumor‐bearing animals. Autonomous tumors had only one‐third the amount of triglycerides present in normal kidneys and hormone‐dependent tumors contained intermediate levels. Host kidneys of animals bearing either primary or transplanted tumors contained no more than 50–60% of the triglyceride level found in normal kidneys. In contrast to triglycerides, cholesteryl esters in primary tumors were 200 times higher than in normal kidneys, exhibited a successive decline in hormone‐dependent and ‐independent tumors, but remained 15 times higher in autonomous carcinomas than in normal kidneys. Cholesterol levels were similar in primary tumors, normal kidneys, and host kidneys of animals bearing renal tumors; however, both hormone‐dependent and ‐independent neoplasms had only one‐half to two‐thirds as much cholesterol as normal kidneys. Total phospholipid levels in primary and transplanted carcinomas were about one‐half those in normal kidneys. Host kidneys of animals bearing primary and transplanted, hormone‐dependent neoplasms also contained lower phospholipid levels than normal kidneys, but renal tissues from animals with autonomous tumors contained similar levels to those found in kidneys from normal hamsters. The phospholipid composition of primary and transplanted renal tumors was similar, but different from that of normal kidneys, mainly in increased percentages of phosphatidylcholine and decreased percentages of sphingomyelin.
T lymphoma variants with specifically altered growth in semi‐solid media
Authors: Claverie Jean‐Michel , Cohn Melvin 
Published: February 2007

AbstractThe relationship between the tumorigenic potential and the cloning efficiency of T lymphoma BW5147 in semi‐solid media has been studied. Two stable variants exhibiting a 30‐fold decrease of their cloning efficiencies in agarose an methycellulose media were independently isolated by negative selection with FuDr. These variants show no alteration of their growth properties in liquid medium and are still able to proliferate in liquid suspension over a bottom layer of agarose. This new phenotype is not correlated with any decreased tumorigenicity in syngeneic AKR/J mice.
Metastatic malignant melanoma: Regression induced by combined treatment with interferon [HuIFN‐α(Le)] and cimetidine
Authors: Flodgren P. , Borgström S. , Jönsson P. E. , Lindström C. , Sjögren H. O. 
Published: February 2007

AbstractWith the aim of potentiating the anti‐tumour effect of interferon in metastatic malignant melanoma by concomitant inhibition of suppressor T cells, oral cimetidine (histamine‐2 receptor antagonist) medication was added to interferon (HuIFN‐α(Le)) therapy in a series of 20 patients. While no objective tumour responses were recorded with interferon treatment alone administered intramuscularly or intratumorally, six patients had objective tumour regressions on subsequent combined therapy. Five out of eight patients with metastases confined to skin and subcutaneous tissue had complete tumour regressions while one patient with skin and lung metastases achieved an extensive partial regression of the skin tumour and a complete roentgenological regression of the lung metastasis. Three additional patients attained a stable disease status for prolonged periods of time. Histopathotogical examinations confirmed disappearance and/or degeneration of melanoma cells and demonstrated a marked lymphocyte infiltration in tumour sites of the patients with objective tumour regression.
Epstein‐Barr virus‐associated antibody pattern in untreated non‐Hodgkin lymphoma patients. Relationship to clinical variables and lymphocyte functions
Authors: Lindemalm C. , Biberfeld P. , Björkholm M. , Henle G. , Henle W. , Holm G. , Johansson B. , Klein G. , Mellstedt H. 
Published: February 2007

AbstractSera from 296 unselected and untreated patients with non‐Hodgkin lymphoma (NHL) classified according to the Rappaport and the Kiel systems were analzyed for antibodies to Epstein‐Barr virus (EBV). The aim of the study was to determine whether antibody spectra and liters to EBV‐coded antigens correlated to clinical and immunological variables and whether the liters were of any prognostic significance. Increased antibody titers to EB viral capsid antigen (VCA) and slightly raised titers to early antigens (EA) of the diffuse (D) and restricted (R) types were noted frequently. Anti‐VCA antibody titers correlated to clinical stage and age of the patients but not to histological. subgroups according to the Rappaport or the Kiel classification systems. However, and‐VCA titers ⩾ 1:2560 were seen only in diffuse lymphomas according to the Rappaport and in non‐follicle cell‐derived lymphomas according to the Kiel classifications. Patients with complement‐receptor‐positive diffuse lymphomas had higher anti‐VGA titers than complement‐receptor‐positive nodular cases. Anti‐VCA titers also correlated positively to serum IgG levels (p <0.01). Total number of lymphocytes separated from peripheral blood and mitogen induced (ConA, PWM) DNA synthesis were recorded before treatment in 54 of the patients. The patients exhibited a significant lymphocytopenia as well as a significantly reduced lymphocyte response to mitogens (p <0.001) compared to healthy controls. Elevated anti‐VCA titers and anti‐EA titers correlated to a good mitogen‐induced lymphocyte response (p <0.05). Only anti‐D 1:40 at diagnosis predicted a poor prognosis.
Differences in the peripheries of Lewis lung tumor cells growing in different sites in the mouse
Authors: Harlos James P. , Weiss Leonard 
Published: February 2007

AbstractThe peripheries of Lewis lung (3LL) tumor cells growing in different organs of the mouse were studied by cell electrophoresis and electron microscopic quantitation of colloid iron hydroxide (CIH) adsorption before and after incubation with neuraminidase. The results show that cells growing in the kidney after direct injection have significantly higher anodic mobilities than cells growing in the subcutaneous sites from which they were derived, or in intramuscular sites, liver or spleen. The proportional contributions of cell surface sialic acids were similar in all sites. Electron microscopy of cells reacted with CIH indicates that the increased surface charge density of the tumor cells growing in the kidney is due to the presence of increased densities of non‐CIH‐binding ionogenic groups. Before neuraminidase treatment, the surface distribution patterns of CIH were indistinguishably random for 3LL cells growing in all sites. After neuraminidase treatment, significantly more clustering of CIH particles was observed on 3LL cells with a history of growth in the kidney than in subcutaneous sites. The changes observed in the 3LL cells growing in the kidney were irreversible, and persisted on multiple back‐transplantation to subcutaneous sites. Detailed analysis of the results shows the changes to be due to the preferential selection of a subpopulation consisting of approximately 10% of the original (subcutaneous) tumor‐cell populaton. This evidence for an irreversible site‐induced selection of a pre‐existing sub‐population of 3LL cells contrasts with the reversible (modulation) site‐induced adaptation previously observed by us in Walker‐256 cancer cells, and therefore indicates that both selective and adaptive processes can occur. Even in the case of the 3LL cells, site‐specific selection is not general, since the changes were observed in tumors growing in the kidney but not in the other anatomic sites. At present we cannot comment on the relevance of these reported changes to naturally occurring metastasis.
Effects on metastases of drug therapy during developing and established tumor immunity
Authors: Vaage Jan , Costanza Mary E. 
Published: February 2007

AbstractThe effectiveness of treatment with cyclophosphamide, methotrexate and 5‐fluorouracil was studied during the s.c. and pulmonary growth of syngeneic C3H/He mammary carcinomas. Treatments with cyclophosphamide, methotrexate and 5‐fluorouracil during the primary induction of immunity against a mouse mammary carcinoma inhibited the growth of the tumor, but also inhibited the development of an effective immune resistance against subsequent implants of the same tumor. However, drug treatments of mice with estatablished tumor immunity gave added benefit without detectable depression of immune resistance.