bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation, characterization and evaluation of 11 autosomal STRs suitable for population studies in black and gold howler monkeysAlouatta caraya
Authors: OKLANDER LUCIANA I. , ZUNINO GABRIEL E. , DI FIORE ANTHONY , CORACH DANIEL 
Published: November 2006

AbstractWe identified 11 polymorphic microsatellite markers forAlouatta caraya. Three markers were isolated from an enriched genomic library ofA. caraya(AC14, AC17, AC45), five were previously described inHomo sapiens(TGMS1, TGMS2, D5S117, D8S165, D17S804), and three were identified forLagothix lagotricha(1110, 1118, 157). Forty‐eight individuals from one Argentinean population were genotyped, yielding heterozygosity values between 0.146 and 0.792. These markers provide an exclusion power of 0.922 when neither parent is known (0.992 when one parent is known) and are suitable for parentage analysis, population genetics and phylogeographical studies ofA. caraya,the southernmost primate in the New World.
New microsatellite markers for the snow crabChionoecetes opilio(Brachyura: Majidae)
Authors: AN H. S. , JEONG J. H. , PARK J. Y. 
Published: November 2006

AbstractNine novel microsatellite loci were isolated fromChionoecetes opilioby screening an enriched genomic library using nonradioactive polymerase chain reaction techniques, and the polymorphisms were examined to estimate genetic variability. The genetic variabilities varied depending on the locus. All loci were found to be polymorphic with an average of 9.7 alleles per locus (range 3–25). The observed and expected heterozygosities ranged from 0.80 to 0.98 and from 0.56 to 0.95, respectively. Five loci showed significant Hardy–Weinberg disequilibrium at theP < 0.05 level. The high variabilities revealed in this study suggest that these microsatellite loci should provide useful markers for genetic variation monitoring ofC. opilio.
Isolation and characterization of polymorphic microsatellite loci for the invasive plant Cytisus scoparius
Authors: KANG MING , LOWE ANDREW , BUCKLEY YVONNE 
Published: November 2006

AbstractScotch broom (Cytisus scoparius) is a serious weed of natural and pastoral ecosystems and is invasive in many countries worldwide. Eight polymorphic microsatellite loci were developed and characterized for this species. The number of alleles per locus ranged from five to 23. The range of observed heterozygosity was between 0.207 and 0.928. These microsatellite markers will be useful tools for studies of population genetics in the native and invasive range of this species.
Isolation and characterization of microsatellite markers in Labiotermes labralis (Isoptera, Termitidae, Nasutitermitinae)
Authors: HARRY M. , ROY V. , MERCIER A. , LIVET A. , GARNIER E. , BOUSSERRHINE N. , DEMANCHE C. 
Published: November 2006

AbstractWe report here on the development of six polymorphic microsatellite loci for Labiotermes labralis. This soil‐feeding species is restricted to the Neotropical rainforest and then could represent a major candidate for being a bio‐indicator of anthropic disturbance resulting in the forest fragmentation. The microsatellite loci were isolated and characterized using a microsatellite‐enriched genomic library. The primers were tested on a French Guyanese population of L. labralis, represented by the sampling of one soldier in 22 nests. The primers were also tested against nine species of the same Nasutitermitinae subfamily and were successfully amplified at five loci in Armitermes minutus.
Isolation and characterization of polymorphic trinucleotide microsatellites of the polyploid crucian carp (Carassius auratus)
Authors: JUNG JONGWOO , LEE EUNJUNG , KIM WON 
Published: November 2006

AbstractIn this study, we isolated and characterized nine polymorphic trinucleotide microsatellites (CAG and CCT) from the crucian carp (Carassius auratus). The number of alleles per locus ranged from two to six. Five loci showed a significantly excess homozygosity, and a genetic linkage between CAL0102 and CAL0495 was strongly suggested. Our results confirmed the triploidy of Korean individuals, and the microsatellites were found to be useful for analysing the allelic state of the polyploid crucian carp.
Characterization of nine microsatellite loci on endemic kangaroo rats Dipodomys simulans peninsularis from southern Baja California Peninsula
Authors: VEGA A. MUNGUÍA , ESTRELLA R. RODRÍGUEZ , BLÁZQUEZ M. C. 
Published: November 2006

AbstractNine polymorphic microsatellite loci were isolated to investigate the effects of habitat fragmentation on gene flow and genetic variation of the peninsular kangaroo rat Dipodomys simulans peninsularis on agricultural landscape from the Baja California Peninsula. The markers had an average of 9.11 alleles per locus (range 2–14), with mean observed and expected heterozygosities 0.646 (range 0.333–0.900) and 0.788 (range 0.284–0.932), respectively. No evidence of linkage disequilibrium was found across pairs of loci, and only one locus exhibited evidence of null alleles.
Development and characterization of nine polymorphic microsatellite markers in the sand goby Pomatoschistus minutus (Gobiidae)
Authors: LARMUSEAU M. H. D. , HELLEMANS B. , VAN HOUDT J. K. J. , VOLCKAERT F. A. M. 
Published: November 2006

AbstractA microsatellite‐enriched genomic library was constructed for the sand goby, Pomatoschistus minutus (Pallas 1770), and nine polymorphic DNA microsatellite markers of high quality were successfully optimized. Characterization of 96 individuals from the Vaccarès lagoon (France) showed moderate to high levels of polymorphism (two to 54 alleles). All the markers conformed to Hardy–Weinberg equilibrium and showed no evidence of null alleles, large allele dropout, stuttering and linkage disequilibrium between pairs of loci. These markers successfully amplify in three closely related species and can be employed to investigate population genetic structure and to clarify paternity in Pomatoschistus species.
Characterization and the broad cross‐species applicability of 20 anonymous nuclear loci isolated from the Taiwan Hwamei (Garrulax taewanus)
Authors: LIN RONG‐CHIEN , YAO CHENG‐TE , LO WEN‐SUI , LI SHOU‐HSIEN 
Published: November 2006

AbstractWe isolated 20 anonymous nuclear loci (8556 bp in total) from the Taiwan Hwamei (Garrulax taewanus), an endemic songbird of Taiwan. A panel of nine to 15 individuals with unknown relationship was used to characterize polymorphism of these loci. We identified 46 single nucleotide polymorphic sites (SNPs) in 15 polymorphic loci. Frequency of SNPs was one per every 186 bp in average. Nucleotide diversity, θ, ranged from 0.00054 to 0.00371 per locus. We also tested cross‐species applicability of these loci on 17 species from eight different passerine families. All 20 loci could be successfully amplified (ranged from one to 16 species, mean = 7.9 species).
Isolation and characterization of microsatellite markers for Sabatia campestris (Gentianaceae), an Illinois state‐endangered plant
Authors: LYONS‐SOBASKI S. A. , BEEVER J. E. 
Published: November 2006

AbstractSabatia campestris is a regionally endangered prairie plant. Eight polymorphic loci have been generated for S. campestris (Gentianaceae) using non‐enriched and enriched libraries. Polymorphism was quantified from an isolated population from central Illinois yielding mean observed and expected heterozygosities of 0.723 and 0.801, respectively. The number of alleles per locus for this population ranged from three to 25 with a median of 15.5. These genetic markers will be used to determine population genetic structure of Illinois populations as well as to estimate fine‐scale gene flow rates.
Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database
Authors: KONG Q. , XIANG C. , YU Z. , ZHANG C. , LIU F. , PENG C. , PENG X. 
Published: November 2006

AbstractSimple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are valuable markers because they represent transcribed regions and often have putative functions. We mined and characterized microsatellites in melon ESTs. Three hundred and eighty‐three SSR loci were identified in 309 of 3188 unigenes assembled by 5747 EST and mRNA sequences in GenBank with occurring frequency of 1/4.7 kb. Twenty‐two polymorphic EST‐SSR markers were developed with the mean allele number of 2.9 per locus and mean expected heterozygosity of 0.442. Amplification products were also detected by 15 pairs of primer in Cucumis sativus. Those informative EST‐SSR markers can be used in melon genetic improvement projects.
Microsatellite markers for the polygamous termite Nasutitermes corniger (Isoptera: Termitidae)
Authors: ATKINSON LYNN , ADAMS ELDRIDGE S. , CROZIER ROSS H. 
Published: November 2006

AbstractWe developed eight highly variable microsatellite markers for the termite Nasutitermes corniger. Allele number per locus ranged from nine to 34, and expected heterozygosity from 0.45 to 0.94, in samples from seven sites in the former canal zone of Panama. The utility of these markers was assessed for five congeners varying in phylogenetic distance to N. corniger. The markers will be useful for fine‐scale examination of population and colony genetic structure in N. corniger and other closely related species.
A fast and inexpensive DNA extraction/purification protocol for brown macroalgae
Authors: HOARAU GALICE , COYER JAMES A. , STAM WYTZE T. , OLSEN JEANINE L. 
Published: November 2006

AbstractHere we describe a rapid method for extracting DNA from dried brown algae material using a microtitre plate system in conjunction with a milling instrument. The method allows the preparation of nuclear and organelle DNA of quality suitable for polymerase chain reaction amplification. It combines high throughput with low cost per sample: DNA from 192 samples can be extracted in c. 3 h for < €0.40 per sample, nearly tenfold cheaper than commercially available kits. Furthermore, by using microtitre plates, efficient storage and downstream processing is facilitated.
The determination of multiple microsatellite genotypes and DNA sequences from a single pollen grain
Authors: MATSUKI Y. , ISAGI Y. , SUYAMA Y. 
Published: November 2006

AbstractPollen plays important roles in the reproduction and gene flow of flowering plants, and its haploid DNA sequence provides useful information for studies of plant evolution and genealogy. We describe a new method for multiple microsatellite genotyping and DNA sequencing from a single pollen grain. The haploid DNA was extracted from a single pollen grain by using a simple DNA extraction method, and multiple microsatellite genotypes and DNA sequences of multiple chloroplast loci were determined. Using nine pairs of microsatellite primers, more than 90% of genotypes were successfully determined, and 71% and 100% of DNA sequences were determined at two chloroplast DNA loci, the trnL intron region and the trnL/trnF intergenic spacer region, respectively. This simple method of genetic analysis for a single pollen grain will facilitate detailed study of pollination, evolution and genealogy.
A select panel of polymorphic microsatellite loci for individual identification of snow leopards (Panthera uncia)
Authors: WAITS LISETTE P. , BUCKLEY‐BEASON VALERIE A. , JOHNSON WARREN E. , ONORATO DAVE , MCCARTHY TOM 
Published: November 2006

AbstractSnow leopards (Panthera uncia) are elusive endangered carnivores found in remote mountain regions of Central Asia. New methods for identifying and counting snow leopards are needed for conservation and management efforts. To develop molecular genetic tools for individual identification of hair and faecal samples, we screened 50 microsatellite loci developed for the domestic cat (Felis catus) in 19 captive snow leopards. Forty‐eight loci were polymorphic with numbers of alleles per locus ranging from two to 11. The probability of observing matching genotypes for unrelated individuals (2.1 × 10−11) and siblings (7.5 × 10−5) using the 10 most polymorphic loci was low, suggesting that this panel would easily discriminate among individuals in the wild.
Microsatellite DNA markers for analysis of population structure in the sea urchin Centrostephanus rodgersii
Authors: BANKS SAM C. , PIGGOTT MAXINE P. , WILLIAMSON JANE E. , BEHEREGARAY LUCIANO B. 
Published: November 2006

AbstractWe describe the development of 13 variable microsatellites developed to investigate population structure and dispersal in the sea urchin Centrostephanus rodgersii. This species is the dominant grazing herbivore in southeast Australian coastal waters and has the ability to modify benthic community structure. The microsatellites we identified showed a range of allele numbers (4–21) and expected heterozygosity (0.32–0.91) in two sampled populations. Contrary to previous findings in free‐spawning marine invertebrates, genotype proportions in neither population deviated significantly from Hardy–Weinberg expectations.
Characterization of SSRs from the American lobster, Homarus americanus
Authors: HODGINS‐DAVIS ANDREA , ROBERTS STEVEN , COWAN DIANE F. , ATEMA JELLE , BENNIE MICHAEL , AVOLIO CARLA , DEFAVERI JACQUELIN , GERLACH GABRIELE 
Published: November 2006

AbstractThe American lobster (Homarus americanus), a commercially important benthic marine crustacean, is widely distributed along the continental shelf of the western North Atlantic. The population substructure of this species remains poorly understood despite its economic value. Informative markers are required to clarify relationships between local populations. To this end, we developed eight polymorphic short sequence repeats (SSR) for the American lobster, which were derived from expressed sequence tags. Additionally, we tested four SSRs previously identified for the Norway lobster (Nephrops norvegicus L.) for cross‐species utility; only one of these showed polymorphism.
The absence of lateral congruency between sighting dominance and the eye with better visual acuity
Authors: Pointer Jonathan S. 
Published: November 2006

AbstractThe notion that in subjects with normal binocular vision (i.e. non‐strabismic cases) the eye showing sighting dominance will also most likely be that individual's better‐sighted eye has proved to be persistent. A review and analysis of the literature has now demonstrated, across several population groups, the fallacy of this belief. In fact, the occurrence of lateral congruency between sighting dominance and the eye with better visual acuity is at a statistical level no greater than chance would predict. Some clinical implications of this outcome are considered.
The Later Medieval English Urban Household
Authors: Rees Jones Sarah , Riddy Felicity , Beattie Cordelia , Carpenter Charlotte , Holford Matthew , McClure Lara , Williams Sarah , Rimmer Jayne , Goldberg Jeremy , Hamblen Bethany , Davis Isabel , Moss Rachel , Tai Wanchen , Kane Bronach , McLean Kate 
Published: November 2006

AbstractHome is one of the most emotive words in any language but our experience of being at home is historically and culturally specific. This article reviews a range of recent scholarship on the later medieval English urban household in the disciplines of History, Literary criticism and Archaeology. It has been composed collaboratively by the members of the York Medieval Household Research Group. After an introduction tracing the wider context in which all histories of domesticity are located, we focus in on our particular period through a close study of contemporary later medieval vocabularies of homeliness. This is followed by a sequence of short thematic sections addressing the historiography of different aspects the demographic structure, ideological construction and daily and emotional life of the household as reflected in the current interests of members of the group.
A Clinical Study of Migraine Evolution
Authors: Pryse‐Phillips William , Aubé Michel , Bailey Peter , Becker Werner J. , Bellavance Andre , Gawel Marek , Giammarco Rose , Mackie Gordon , Purdy R. Allan , Robinson Gordon , Shapero Gary , Wilson Keith 
Published: November 2006

Background.—The buildup time of migraine headaches has not been well delineated in publications to date and we are aware of patients whose migraines last well over 72 hours. More concentration on these factors in the assessment of patients might lead to more appropriate therapeutic choices.Method.—Prospective ascertainment of such data through a questionnaire completed by 253 informed and willing patients with IHS migraine with or without aura consulting Canadian headache specialists. Data were electronically sent to a central computer from each center, tabulated and analyzed using standard statistical parameters.Results.—In 253 patients with migraine ascertained using applied IHS criteria, nausea was a feature in over 90% of cases, especially in those with aura. This inhibited the ingestion of oral medications in about a quarter of all subjects. The time to build from no pain to moderate/severe pain was shorter in subjects with auras and was less than 2 hours in 97% of those with and 86% of those without auras. However, we also identified a group of subjects with migraine (over 10% of all) in whom the build time to maximum pain is delayed for over 2 hours. Nausea was experienced by 91.7% of subjects, slightly but significantly later in those without auras. While most headaches in each group lasted from 4 to 72 hours, 24.3% of those with and 20.6% of those without aura expected to experience pain for more than 72 hours, while in untreated cases disability due to pain, nausea, or malaise usually persisted for over 3 days in 24.3% and 16.7% of those with and without aura, respectively. One‐fifth of migraineurs may be in pain and/or disabled by accompanying symptoms for over 3 days in a typical migraine attack. Over half of our subjects reported that their medications worked well or excellently.Conclusions.—Attacks of migraine in real‐life clinical situations vary somewhat from the IHS criteria in that they are more often associated with nausea that interferes with oral therapy; can persist for over 72 hours; may have slow (>2 hours) buildup to maximum pain in 10% of cases; and may cause disability for over 3 days. Nevertheless, current therapeutic regimens (including prescribed medications) work well for a substantial majority.
Gene Expression Profiling in Cluster Headache: A Pilot Microarray Study
Authors: Sjöstrand Christina , Duvefelt Kristina , Steinberg Anna , Remahl Ingela Nilsson , Waldenlind Elisabet , Hillert Jan 
Published: November 2006

Background.—Cluster headache (CH) is a primary neurovascular headache disorder characterized by attacks of excruciating pain accompanied by ipsilateral autonomic symptoms. CH pathophysiology is presumed to involve an activation of hypothalamic and trigeminovascular systems, but inflammation and immunological mechanisms have also been hypothesized to be of importance.Objective.—To identify differentially expressed genes during different clinical phases of CH, assuming that changes of pathophysiological importance would also be seen in peripheral venous blood.Methods.—Blood samples were drawn at 3 consecutive occasions from 3 episodic CH patients: during attacks, between attacks and in remission, and at 1 occasion from 3 matched controls. Global gene expression was analyzed with microarray tehnology using the Affymetrix Human Genome U133 2.0 Plus GeneChip® Set, covering more than 54,000 gene transcripts, corresponding to almost 22,000 genes. Quantitative RT‐PCR on S100P gene expression was analyzed in 6 patients and 14 controls.Results.—Overall, quite small differences were seen intraindividually and large differences interindividually. However, pairwise comparisons of signal values showed upregulation of several S100 calcium binding proteins; S100A8 (calgranulin A), S100A12 (calgranulin C), and S100P during active phase of the disease compared to remission. Also, annexin A3 (calcium‐binding) and ICAM3 showed upregulation. BIRC1 (neuronal apoptosis inhibitory protein), CREB5, HLA‐DQA1, and HLA‐DQB1 were upregulated in patients compared to controls. The upregulation of S100P during attack versus remission was confirmed by quantitative RT‐PCR analysis.Conclusions.—The S100A8 and S100A12 proteins are considered markers of non‐infectious inflammatory disease, while the function of S100P is still largely unknown. Furthermore, upregulation of HLA‐DQ genes in CH patients may also indicate an inflammatory response. Upregulation of these pro‐inflammatory genes during the active phase of CH has not formerly been reported. Data from this pilot microarray study provide a basis for further studies in CH.
Change of Excitability in Brainstem and Cortical Visual Processing in Migraine Exhibiting Allodynia
Authors: Shibata Koichi , Yamane Kiyomi , Iwata Makoto 
Published: November 2006

Background.—Clinical and neurophysiological manifestations of information processing associated with central sensitization are little known. Allodynic migraine (AM) can be caused by the sensitization of trigeminal neuron, but no study has reported on AM between attacks using blink reflex (BR) and pattern‐reversal visual evoked potentials (PVEPs).Objective.—We explored the characteristics of AM between attacks associated with central sensitization using BR and PVEP.Methods.—We recruited 13 patients with interictal AM and 15 patients with nonallodynic migraine (NA), and 30 healthy subjects (HS). BRs were obtained using paired pulses delivered at the interstimulus interval (ISI) of 150, 300, and 500 ms. The ratio of the area in the R2 of the second to R2 of the first shock was measured for each ISI. PVEP were recorded with 2 spatial frequencies (0.5 and 4.0 cpd) and 2 low and high contrasts (29% and 98%, respectively). Amplitudes of P100 were measured.Results.—For BR, there were no significant differences in the ratio of the area of the R2 between the sides of stimulation, and the sides of headache. AM patients had less suppression of the R2 at the ISI of 150 and 300 ms when compared with the NA patients and HS. For PVEP, at 0.5, there were significant differences of amplitude between AM patients and HS, and between NA patients and HS in low and high contrast. At 4.0 cpd, there were significant differences of amplitude between AM patients and HS in low contrast, and between AM patients and HS, and NA patients and HS in high contrast. In AM patients, there was a significant difference of amplitude ratio between 0.5 and 4.0 cpd.Conclusions.—Our BR and PVEP study showed that migraine patients exhibiting allodynia may show central sensitization of brainstem trigeminal neuron and have contrast modulating dysfunction during the cortical visual processing of striate and extrastriate on visual cortex in‐between attacks.