bluuebunny/crossref_metadata_2025_split huggingface dataset:
Signalling responses in the bark and foliage of canker‐susceptible and ‐resistant cypress clones inoculated with Seiridium cardinale
Authors: Scimone Giulia , Pisuttu Claudia , Cotrozzi Lorenzo , Danti Roberto , Nali Cristina , Pellegrini Elisa , Tonelli Mariagrazia , Della Rocca Gianni 
Published: March 2024

AbstractThe necrotrophic fungus Seiridium cardinale is the main responsible for Cypress Canker Disease (CCD), a pandemic affecting many Cupressaceae worldwide. The present study aims to elucidate the signalling of the early responses in the bark and foliage of CCD‐susceptible and ‐resistant C. sempervirens clones to S. cardinale inoculation (SI and RI, respectively). In the bark of SI, a peaking production of ethylene (Et) and jasmonic acid (JA) occurred at 3 and 4 days post inoculation (dpi), respectively, suggesting an attempted plant response to the pathogen. A response that, however, was ineffective, as confirmed by the severe accumulation of malondialdehyde by‐products at 13 dpi (i.e., lipid peroxidation). Differently, Et emission peaked in RI bark at 3 and 13 dpi, whereas abscisic acid (ABA) accumulated at 1, 4 and 13 dpi, resulting in a lower MDA accumulation (and unchanged levels of antioxidant capacity). In the foliage of SI, Et was produced at 1 and 9 dpi, whereas JA and salicylic acid (SA) accumulated at 1 and 3 dpi. Conversely, an increase of ABA and SA occurred at 1 dpi in the RI foliage. This outcome indicates that some of the observed metabolic alterations, mainly occurring as local defence mechanisms, might be able to gradually shift to a systemic resistance, although an accumulation of MDA was observed in both SI and RI foliage (but with an increased antioxidant capacity reported only in the resistant clone). We believe that the results reported here will be useful for the selection of clones able to limit the spread and damage of CCD.
Gender effect in affective processing: Alpha EEG source analysis on emotional slides and film‐clips
Authors: Romeo Zaira , Angrilli Alessandro , Spironelli Chiara 
Published: March 2024

AbstractPast research on gender‐related brain asymmetries in emotions was limited and not univocal. The present study analyzed EEG alpha activity (indexing cortical de‐activation) from 64 scalp sites in 20 women and 20 men during a counterbalanced block presentation of emotional slides and short video‐clips. Stimuli consisted of 45 brief clips of 13 s, divided into 15 erotic (pleasant), 15 neutral and 15 fear (unpleasant) contents. Slides consisted in 45 photo shots (presented for 13 s each) extracted from the videos. As expected, women perceived fear stimuli as more arousing and more unpleasant compared to men. Alpha EEG source analysis revealed gender effects depending on stimulus. Emotional film‐clips elicited in both groups a pattern of greater right than left occipital activation. Instead, emotional pictures activated opposite occipital regions, as women showed greater activation in the left, men in the right hemisphere. Men also showed greater activation to Erotic compared to Fear stimuli (i.e., pictures/clips) in the posterior parietal complex. Results point to the relevance of emotional stimulus type to reveal gender effects: clips are ecological, dynamic and engaging, and forced a unified pattern of emotional responses that reset individual differences. Emotional pictures, less engaging, allowed individual differences to emerge and interact with the stimulus category.
Ophthalmic findings associated with Australian tick paralysis (holocyclotoxicity) in hospitalized domestic dogs and cats
Authors: Reynolds B. D. , Nagel H. G. , Perry E. , Whittaker C. J. , Caruso K. A. , Annear M. J. , Irving W. M. , McCarthy P. M. , Dion A. , Yi J. M. S. , Hall E. , Smith J. S. 
Published: March 2024

AbstractObjectiveTo describe ophthalmic findings in hospitalized canine and feline patients with tick paralysis (TP) and investigate possible predisposing factors.Animals StudiedForty‐seven dogs and 28 cats hospitalized with TP assessed with an ophthalmic examination performed by an ABVO resident.MethodsDogs and cats were hospitalized with TP from October 2021 to January 2022 and had an ophthalmic examination performed by an ABVO resident. Patient signalment data, information regarding tick number and location, hospitalization duration, medications used, and patient paralysis grades were recorded. Statistical analysis was performed to correlate findings.ResultsCorneal ulcers developed in up to 34.8% of dogs and up to 42.9% of cats hospitalized with TP. An absent palpebral reflex ipsilaterally increased the odds of a concurrent corneal ulcer being present by 14.7× in dogs and 20.1× in cats (p < .0001). Palpebral reflexes were absent in 38.3% of dogs and 35.7% of cats hospitalized with TP and were correlated with more severe gait paralysis (p = .01) and respiratory paralysis (p = .005) in dogs, and respiratory paralysis in cats (p = .041). STT‐1 findings <10 mm/min were present in 27.7% of dogs and 57.1% of cats examined and were associated with increasing gait paralysis (p = .017) and respiratory paralysis (p = .007) in dogs, and increasing gait paralysis in cats (p = .017).ConclusionsSimple corneal ulcers, loss of a complete palpebral reflex, and reduced STT‐1 scores frequently occurred in dogs and cats hospitalized for TP. The frequency of these findings increased as the degree of patient paralysis increased.
Weed seedbank as influenced by tillage and herbicide in direct seeded rice‐mustard cropping sequence in lateritic soil of eastern India
Authors: Jaiswal Deepak Kumar , Duary Buddhadeb , Kumar Ravi Ranjan , Nath Chaitanya P. 
Published: March 2024

AbstractThe above‐ground community dynamics of weeds represent the soil weed seedbank in an agro‐ecosystem. Hence, understanding the seedbank will offer an idea for future weed management tactics. We aimed to determine the density of weed seedbank and emergence of major weeds in direct seeded rice (DSR) (Oryza sativa L.)—mustard (Brassica campestris L. var. yellow sarson) cropping sequence under varied tillage and herbicide combinations. The present study on weed seedbank was conducted using seedling emergence method after collecting the soils at the harvest of mustard at the completion of 2 years field experimentation. The experiment was conducted in split‐plot design with two tillage practices [zero tillage (ZT) and conventional tillage (CT)] in main plot and eight weed management practices in DSR [oxadiargyl followed by (fb) bispyribac‐sodium, penoxsulam + cyhalofop‐butyl, oxadiargyl fb penoxsulam + cyhalofop‐butyl, fenoxaprop‐p‐ethyl + ethoxysulfuron, oxadiargyl fb fenoxaprop‐p‐ethyl + ethoxysulfuron, pendimethalin fb bispyribac‐sodium, unweeded control, and weed free check] as subplot. The carry‐over effect of tillage and weed management practices were recorded in mustard crop. Seed emergence study revealed that ZT reduced the emergence of grassy weeds (by 13.7%) and total weed emergence (19.5%) in comparison to the CT (p < 0.05). However, emergence of broadleaf weeds was 24.5% higher in ZT than in CT. The higher infestation was recorded for Ludwigia parviflora (Jacq.) Raven under penoxsulam + cyhalofop‐butyl, fenoxaprop‐p‐ethyl + ethoxysulfuron, Cyperus iria (L.) under fenoxaprop‐p‐ethyl + ethoxysulfuron, and Eleusine indica (L.) Gaertn. under pendimethalin fb bispyribac‐sodium, oxadiargyl fb bispyribac‐sodium. Sole application of post‐emergence (POE) herbicide decreased the total weed emergence by 22.2%–28.1% than unweeded control (p < 0.05). Whereas, sequential application of PRE + POE herbicides decreased the total weed emergence by 32.8%–36.9% than unweeded control (p < 0.05). Notably, sedge weeds such as Cyperus iria (L.) and Fimbristylis miliacea (L.) Vahl were lower in ZT than in CT under herbicide treated plots (by 66.6%–84.3%) than unweeded control. Thus, ZT with oxadiargyl 90 g a.i. ha−1 fb fenoxaprop‐p‐ethyl + ethoxysulfuron (90 + 15 g a.i. ha−1) as tank‐mix can be effectively used to lower the weed seedbank density vis‐à‐vis managing complex weed flora in DSR and rice‐mustard cropping sequence.
Data Center Environmental Burden Reduction Through On-Site Renewable Power Generation
Authors: McMullen M. , Wemhoff A. P. 
Published: March 2024

Abstract
The energy demands from data centers contribute greatly to water scarcity footprint and carbon emissions. Understanding the use of on-site renewable power generation is an important step to gain insight into making data centers more sustainable. This novel study examines the impact of on-site solar or wind energy on data center water scarcity usage effectiveness (WSUE) and carbon usage effectiveness (CUE) at a U.S. county scale for a given data center size, water consumption level, and energy efficiency. The analysis uncovers combinations of specific metrics associated with grid-based carbon emissions and water scarcity footprint that enable predictions of the improvements anticipated when implementing on-site solar or wind energy. The implementation of on-site renewables has the most benefit in reducing carbon footprint in areas with high existing grid-based emissions such as the western side of the Appalachian Mountains (e.g., central and eastern Kentucky). The largest benefit in reducing water scarcity footprint is generally seen in counties with low water scarcity compared to adjacent areas (e.g., northern California).
Optimizing Health-Related Outcomes Following Musculoskeletal Injury Through the Integration of Self-Efficacy Theory and the Fear Avoidance Model
Authors: Walsh Bridget M. , Porter Ke’La H. , Hoch Matthew C. 
Published: March 2024

A primary goal of rehabilitation is to produce optimal health-related quality of life outcomes, which includes returning patients to their desired level of activity. Injury-related fear, kinesiophobia, and fear-avoidance beliefs are psychological factors that negatively impact health-related quality of life and are associated with decreased levels of physical activity. Conversely, increased levels of self-efficacy have been linked to favorable rehabilitation outcomes. To promote full recovery and optimal health-related quality of life, the rehabilitation process should consider addressing self-efficacy and injury-related fear (e.g., kinesiophobia, fear-avoidance beliefs). Therefore, the purpose of this paper is to introduce a theoretical framework which integrates self-efficacy theory and the Fear-Avoidance Model to mitigate injury-related fear and optimize patient outcomes following musculoskeletal injury. The intention of this theoretical framework is to provide clinicians with psychosocial interventions to improve self-efficacy and prevent the progression of injury-related fear at varying times throughout the rehabilitation process.
Young Danish Esports Players Physical Health, and Their Opportunities and Wishes for Physical Activity From Their Esports Club: A Cross-Sectional Study
Authors: Gaasedal Julie , Thøgersen Mia Ricaplaza , Straszek Christian Lund , Guldager Julie Dalgaard 
Published: March 2024

Electronic sports (esports) refer to competitive video gaming at professional and amateur levels. The increasing popularity of esports may lead to an increased tendency toward inactivity and sedentary behavior among players. Currently, 150 min of physical activity (PA) of moderate to high intensity per week is recommended to main a healthy lifestyle. The aim of this study was to investigate whether young esports players are offered to participate in PA and what type of PA they would like to participate in. The level of PA, bodily pain, and sleeping habits were also investigated through a self-reported questionnaire. The median score for PA was 2,850 metabolic equivalent of task minutes per week. One in every five esports players was offered PA by their esports association, and most of them have used this offer. For participants who were not offered PA, the most often called-for type of PA was resistance training, ballgames, and cardiovascular training.
ACE2 acts as a novel regulator of TMPRSS2-catalyzed proteolytic activation of influenza A virus in airway cells
Authors: Heindl Miriam Ruth , Rupp Anna-Lena , Schwerdtner Marie , Bestle Dorothea , Harbig Anne , De Rocher Amy , Schmacke Luna C. , Staker Bart , Steinmetzer Torsten , Stein David A. , Moulton Hong M. , Böttcher-Friebertshäuser Eva 
Published: March 2024

ABSTRACT

The transmembrane serine protease 2 (TMPRSS2) activates the outer structural proteins of a number of respiratory viruses including influenza A virus (IAV), parainfluenza viruses, and various coronaviruses for membrane fusion. Previous studies showed that TMPRSS2 interacts with the carboxypeptidase angiotensin-converting enzyme 2 (ACE2), a cell surface protein that serves as an entry receptor for some coronaviruses. Here, by using protease activity assays, we determine that ACE2 increases the enzymatic activity of TMPRSS2 in a non-catalytic manner. Furthermore, we demonstrate that ACE2 knockdown inhibits TMPRSS2-mediated cleavage of IAV hemagglutinin (HA) in Calu-3 human airway cells and suppresses virus titers 100- to 1.000-fold. Transient expression of ACE2 in ACE2-deficient cells increased TMPRSS2-mediated HA cleavage and IAV replication. ACE2 knockdown also reduced titers of MERS-CoV and prevented S cleavage by TMPRSS2 in Calu-3 cells. By contrast, proteolytic activation and multicycle replication of IAV with multibasic HA cleavage site typically cleaved by furin were not affected by ACE2 knockdown. Co-immunoprecipitation analysis revealed that ACE2-TMPRSS2 interaction requires the enzymatic activity of TMPRSS2 and the carboxypeptidase domain of ACE2. Together, our data identify ACE2 as a new co-factor or stabilizer of TMPRSS2 activity and as a novel host cell factor involved in proteolytic activation and spread of IAV in human airway cells. Furthermore, our data indicate that ACE2 is involved in the TMPRSS2-catalyzed activation of additional respiratory viruses including MERS-CoV.

IMPORTANCE
Proteolytic cleavage of viral envelope proteins by host cell proteases is essential for the infectivity of many viruses and relevant proteases provide promising drug targets. The transmembrane serine protease 2 (TMPRSS2) has been identified as a major activating protease of several respiratory viruses, including influenza A virus. TMPRSS2 was previously shown to interact with angiotensin-converting enzyme 2 (ACE2). Here, we report the mechanistic details of this interaction. We demonstrate that ACE2 increases or stabilizes the enzymatic activity of TMPRSS2. Furthermore, we describe ACE2 involvement in TMPRSS2-catalyzed cleavage of the influenza A virus hemagglutinin and MERS-CoV spike protein in human airway cells. These findings expand our knowledge of the activation of respiratory viruses by TMPRSS2 and the host cell factors involved. In addition, our results could help to elucidate a physiological role for TMPRSS2.
The recruitment of TRiC chaperonin in rotavirus viroplasms correlates with virus replication
Authors: Vetter Janine , Papa Guido , Tobler Kurt , Rodriguez Javier M. , Kley Manuel , Myers Michael , Wiesendanger Mahesa , Schraner Elisabeth M. , Luque Daniel , Burrone Oscar R. , Fraefel Cornel , Eichwald Catherine 
Published: March 2024

ABSTRACT

Rotavirus (RV) replication takes place in the viroplasms, cytosolic inclusions that allow the synthesis of virus genome segments and their encapsidation in the core shell, followed by the addition of the second layer of the virion. The viroplasms are composed of several viral proteins, including NSP5, which serves as the main building block. Microtubules, lipid droplets, and miRNA-7 are among the host components recruited in viroplasms. We investigated the interaction between RV proteins and host components of the viroplasms by performing a pull-down assay of lysates from RV-infected cells expressing NSP5-BiolD2. Subsequent tandem mass spectrometry identified all eight subunits of the tailless complex polypeptide I ring complex (TRiC), a cellular chaperonin responsible for folding at least 10% of the cytosolic proteins. Our confirmed findings reveal that TRiC is brought into viroplasms and wraps around newly formed double-layered particles. Chemical inhibition of TRiC and silencing of its subunits drastically reduced virus progeny production. Through direct RNA sequencing, we show that TRiC is critical for RV replication by controlling dsRNA genome segment synthesis, particularly negative-sense single-stranded RNA. Importantly, cryo-electron microscopy analysis shows that TRiC inhibition results in defective virus particles lacking genome segments and polymerase complex (VP1/VP3). Moreover, TRiC associates with VP2 and NSP5 but not with VP1. Also, VP2 is shown to be essential for recruiting TRiC in viroplasms and preserving their globular morphology. This study highlights the essential role of TRiC in viroplasm formation and in facilitating virion assembly during the RV life cycle.

IMPORTANCE
The replication of rotavirus takes place in cytosolic inclusions termed viroplasms. In these inclusions, the distinct 11 double-stranded RNA genome segments are co-packaged to complete a genome in newly generated virus particles. In this study, we show for the first time that the tailless complex polypeptide I ring complex (TRiC), a cellular chaperonin responsible for the folding of at least 10% of the cytosolic proteins, is a component of viroplasms and is required for the synthesis of the viral negative-sense single-stranded RNA. Specifically, TRiC associates with NSP5 and VP2, the cofactor involved in RNA replication. Our study adds a new component to the current model of rotavirus replication, where TRiC is recruited to viroplasms to assist replication.
Tetramerization is essential for the enzymatic function of the
Pseudomonas aeruginosa
virulence factor UDP-glucose pyrophosphorylase
Authors: Dirr Larissa , Cleeves Sven , Ramón Roth Isabel , Li Linghui , Fiebig Timm , Ve Thomas , Häussler Susanne , Braun Armin , von Itzstein Mark , Führing Jana I. 
Published: March 2024

ABSTRACT

Multidrug-resistant bacteria such as the opportunistic pathogen
Pseudomonas aeruginosa
, which causes life-threatening infections especially in immunocompromised individuals and cystic fibrosis patients, pose an increasing threat to public health. In the search for new treatment options,
P. aeruginosa
uridine diphosphate-glucose pyrophosphorylase (PaUGP) has been proposed as a novel drug target because it is required for the biosynthesis of important virulence factors and linked to pathogenicity in animal models. Here, we show that UGP-deficient
P. aeruginosa
exhibits severely reduced virulence against human lung tissue and cells, emphasizing the enzyme’s suitability as a drug target. To establish a basis for the development of selective PaUGP inhibitors, we solved the product-bound crystal structure of tetrameric PaUGP and conducted a comprehensive structure-function analysis, identifying key residues at two different molecular interfaces that are essential for tetramer integrity and catalytic activity and demonstrating that tetramerization is pivotal for PaUGP function. Importantly, we show that part of the PaUGP oligomerization interface is uniquely conserved across bacterial UGPs but does not exist in the human enzyme, therefore representing an allosteric site that may be targeted to selectively inhibit bacterial UGPs.

IMPORTANCE

Infections with the opportunistic bacterial pathogen
Pseudomonas aeruginosa
are becoming increasingly difficult to treat due to multidrug resistance. Here, we show that the enzyme uridine diphosphate-glucose pyrophosphorylase (UGP) is involved in
P. aeruginosa
virulence toward human lung tissue and cells, making it a potential target for the development of new antibacterial drugs. Our exploration of
P. aeruginosa
(Pa)UGP structure-function relationships reveals that the activity of PaUGP depends on the formation of a tetrameric enzyme complex. We found that a molecular interface involved in tetramer formation is conserved in all bacterial UGPs but not in the human enzyme, and therefore hypothesize that it provides an ideal point of attack to selectively inhibit bacterial UGPs and exploit them as drug targets.
Alternative substitutions of N332 in HIV-1
AD8
gp120 differentially affect envelope glycoprotein function and viral sensitivity to broadly neutralizing antibodies targeting the V3-glycan
Authors: Jeffy Jeffy , Parthasarathy Durgadevi , Ahmed Shamim , Cervera-Benet Héctor , Xiong Ulahn , Harris Miranda , Mazurov Dmitriy , Pickthorn Stephanie , Herschhorn Alon 
Published: March 2024

ABSTRACT

The envelope glycoprotein (Env) trimer on the surface of human immunodeficiency virus type I (HIV-1) mediates viral entry into host CD4
+
T cells and is the sole target of neutralizing antibodies. Broadly neutralizing antibodies (bnAbs) that target gp120 V3-glycan of HIV-1 Env trimer are potent and block the entry of diverse HIV-1 strains. Most V3-glycan bnAbs interact, to a different extent, with a glycan attached to N332, but Asn at this position is not absolutely conserved or required for HIV-1 entry based on the prevalence of N332 in different circulating HIV-1 strains from diverse clades. Here, we studied the effects of amino acid changes at position 332 of HIV-1
AD8
Envs on HIV-1 sensitivity to antibodies, cold exposure, and soluble CD4. We further investigated how these changes affect Env function and HIV-1 infectivity
in vitro
. Our results suggest robust tolerability of HIV-1
AD8
Env N332 to changes, with specific changes that resulted in extended exposure of gp120 V3 loop, which is typically concealed in most primary HIV-1 isolates. Viral evolution leading to Asn at position 332 of HIV
AD8
Envs is supported by the selection advantage of high levels of cell–cell fusion, transmission, and infectivity with high levels of cell surface expression and slightly higher gp120 shedding than most N332 variants. Thus, tolerance of HIV-1
AD8
Envs to different amino acids at position 332 provides increased flexibility to respond to changing conditions/environments and evade the immune system. Modeling studies of the distance between N332 glycan and specific bnAbs were in agreement with N332 glycan dependency on bnAb neutralization. Overall, our studies provide insights into the contribution of specific amino acids at position 332 to Env antigenicity, stability on ice, and conformational states.

IMPORTANCE
Glycan attached to amino acid asparagine at position 332 of HIV-1 envelope glycoproteins is a main target of a subset of broadly neutralizing antibodies that block HIV-1 infection. Here, we defined the contribution of different amino acids at this position to Env antigenicity, stability on ice, and conformational states.