bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of Colletotrichum acutatum in Blueberry Plants in Spain
Authors: Barrau C. , de los Santos B. , Romero F. 
Published: May 2007

An anthracnose disease was observed affecting leaves of high-bush blueberry plants (Vaccinium corymbosum L. ‘Sharpblue’) in small areas within two production fields in Huelva Province of Andalucía, in southwestern Spain. The first symptoms observed in late spring were circular, necrotic lesions, red to salmon in color, and ranging from 3 to 20 mm in diameter. Later, lesions became salmon colored in the center with a brilliant red halo. Fungal isolations were made from the lesions. Infected tissues were surface-disinfected in 1% sodium hypochlorite for 1 min, blotted dry on sterile filter paper, and plated on 2% water agar. The plates were incubated at 25°C for 5 to 10 days. Fungal colonies isolated from the tissues were transferred to potato dextrose yeast agar (PDYA). Only one fungal species was consistently isolated from affected leaf tissues and was identified as Colletotrichum acutatum J.H. Simmonds based on morphological characteristics (2) and enzyme-linked immunosorbent assay (1). Colonies of the fungus on PDYA showed a white-to-gray dense mycelium covered with salmon-colored spore masses. The reverse of the plates was a pink-salmon color. Colony diameter on PDYA averaged 50 mm after 7 days at 25°C. Conidia were hyaline, aseptate, fusiform to cylindrical, and 12.5 × 3.2 μm. Inoculation of leaves and fruits of blueberry cv. Misty with a conidial suspension (106 conidia per ml) of C. acutatum produced lesions on the leaves and fruits similar to those observed on diseased plants in the field. The pathogen was isolated from lesions on inoculated plants. To our knowledge, this is the first report of C. acutatum in high-bush blueberry plants in Spain. References: (1) T. A. Cooke et al. EPPO Bull. 25:57, 1995. (2) B. C. Sutton. The Coelomycetes. CMI, Kew, England, 1980.
First Report of Soybean Rust in South Africa
Authors: Pretorius Z. A. , Kloppers F. J. , Frederick R. D. 
Published: May 2007

In February 2001, rust caused by Phakopsora pachyrhizi Syd. was detected for the first time on soybean (Glycine max (L.) Merr.) near Vryheid in northern KwaZulu-Natal, South Africa. As the season progressed, the disease was also observed in other parts of the province, and epidemic levels were reached in the Karkloof, Cedara, Howick, and Greytown production regions. In affected areas, infection foci gradually increased in size and caused premature yellowing and defoliation of soybean crops, usually after the flowering stage. Typical rust symptoms (3) were produced predominantly on the lower surface of soybean leaves. Soybean rust subsequently spread to Amsterdam and Ermelo in the Highveld region of South Africa. Following emergency registration of triazole compounds, fungicides were commonly used to control soybean rust, especially in the more humid eastern production areas. Available yield data suggested a reduction in kernel mass between 4 and 23%, depending on the cultivar and host growth stage at the time of infection. Urediniospores from the original collection (isolate PREM 57280, Plant Protection Research Institute, Pretoria, South Africa) were 23 to 33 × 15 to 22 μm, indicating that spore dimensions fell within the known range for P. pachyrhizi (3). To confirm pathogenicity, 10 to 15 plants of each of the South African soybean cvs. Pan 589, Pan 780, Pan 854, Octa, and Prima were inoculated with isolate PREM 57280. Primary leaves were sprayed with a suspension of spores in light mineral oil (approximately 1 mg of spores per ml) before incubating plants in the dark in a dew chamber for 16 h. Large, sporulating uredinia, producing typical soybean rust urediniospores, developed on all inoculated plants. Classical and real-time fluorescent polymerase chain reaction assays as well as sequence analysis of the internal transcribed spacer regions verified the identity of isolate PREM 57280 as P. pachyrhizi (2). Since the disease is known to occur in Zimbabwe, Mozambique, and several other African countries (1,3,4), inoculum was most likely introduced by air currents from countries to the north of South Africa. It is highly probable that soybean rust will successfully overwinter in South Africa based on experience in other southern African countries. References: (1) O. A. Akinsanmi and J. L. Ladipo. Plant Dis. 85:97, 2001. (2) R. D. Frederick et al. (Abstr.) Phytopathology 90 (suppl):S25, 2000. (3) G. L. Hartman et al. eds. Compendium of Soybean Diseases, 4th ed. The American Phytopathological Society, St. Paul, MN, 1999. (4) J. B. Sinclair and G. L. Hartman, eds. Soybean Rust Workshop, Publ. 1 College of Agricultural, Consumer, and Environmental Sciences, National Soybean Research Laboratory, Urbana, IL. 1996.
First Report of the Root-Knot Nematode Meloidogyne ardenensis on Lady's Mantle in Norway
Authors: Holgado R. , Hammeraas B. , Karssen G. 
Published: May 2007

In August 2000, several small galls were observed on roots of lady's mantle (Alchemilla acutiloba Opiz) from an old grassland near Kristiansund in northern Norway. These plants, without clear above-ground symptoms, were infected with an unknown root-knot nematode. Soil and plant samples were collected in July 2001 at the previous year's location and sent for identification to the Plant Protection Service in Wageningen, the Netherlands. The smooth elongated galls included several males, swollen adult females with protruding small egg masses and hatching second-stage juveniles. Males and second-stage juveniles also were isolated from adhering soil. The root-knot nematode was identified as Meloidogyne ardenensis Santos (1). Identification was based on female, male, and second-stage juvenile morphology and female isozyme electrophoresis with malate dehydrogenase and esterase. M. ardenensis parasitizes several dicotyledonous hosts, primarily herbaceous and woody plants, and is distributed throughout Europe, with Scotland the most northern area (2). To our knowledge, this is not only the first published report of M. ardenensis in Scandinavia, but also the first report of this species on lady's mantle. References: (1) M. S. N. de A. Santos. Nematologica 13:593, 1968. (2) P. R. Thomas and D. J. F. Brown. Plant Pathol. 30:147, 1981.
Pepper huasteco virus and Pepper golden mosaic virus are Geminiviruses Affecting Tomatillo (Physalis ixocarpa) Crops in Mexico
Authors: Méndez-Lozano J. , Rivera-Bustamante R. F. , Fauquet C. M. , Torre-Almaraz R. De la 
Published: May 2007

Whitefly-transmitted geminivirus diseases cause important losses in several horticultural crops in all areas in Mexico (1). Tomatillo is important in the Mexican diet since it is widely used to prepare many types of salsas and other dishes. As a result, tomatillo, also known as tomate verde (green tomato), is cultivated in 29 of 32 states in Mexico, with the main production areas located in the states of Morelos, Puebla, and Michoacán. Leaf samples of 105 tomatillo plants exhibiting yellowing, yellowing mosaic, leaf curl, bunchy top, and stunting were collected from the states of Puebla, Morelos, Estado de México, and Sinaloa. Symptomatic plants were associated with the presence of whiteflies in many fields and suggested a viral etiology. Total DNA extracted from symptomatic tomatillo plants was used as a template in a polymerase chain reaction (PCR)-based geminivirus detection procedure. MP16 and MP82 primers (2) were used to direct the amplification of a segment from the stem-loop structure in the intergenic region (IR) to a conserved region in the coat protein (CP) of begomoviruses (2). Sixty-nine percent (72/105) of the samples produced the expected PCR fragment (400 to 450 bp). Similar results were obtained with a dot-blot hybridization procedure using as a probe the component A of Pepper huasteco virus (PHV) under low stringency conditions. More than 50 PCR products were cloned and sequenced. Sequence analysis (nucleotide level for the IR; amino acid level for the CP) revealed that the tomatillo-infecting geminiviruses clustered into two main groups. The first group showed a high percent identity (average of 95.3% at the CP N terminus) to PHV, whereas the second showed a similarly high percent (average 93.8%) identity to Pepper golden mosaic virus (PepGMV, previously called Texas pepper geminivirus. Both PepGMV and PHV were found in all sampled areas. Although mixed infections (differentiated by the respective IR probes) of PHV and PepGMV were common (61%), single infections were also detected (PHV 27%; PepGMV 10%). The presence of begomoviruses in tomatillo crops has been previously reported (1); however, their identity as PHV and PepGMV was not confirmed. References: (1) I. Torres-Pacheco et al. Phytopathology 86:1186, 1996. (2) P. Umaharan et al. Phytophatology 88:1262, 1998.
Occurrence of Potato Wart Caused by Synchytrium endobioticum on Prince Edward Island, Canada
Authors: De Boer S. H. 
Published: May 2007

During harvest (October 2000) of a 26-ha field of processing potatoes (cv. Russet Burbank) on Prince Edward Island (PEI), a small number of tubers with symptoms of potato wart were found in a 1-ha area of the field. Resting sporangia of Synchytrium endobioticum were present in diseased tissue. Potato wart is not endemic in Canada outside of Newfoundland, where the disease has occurred since 1909, and has been under official quarantine control since 1912 (1). In the United States, the disease was eradicated from Pennsylvania and West Virginia by 1974 and, more recently, was eradicated from Maryland, where its presence had been reconfirmed in 1987 (2). Anecdotal information pertaining to the PEI field suggests that the source of infection may have been infected tubers from Newfoundland that were grown in this portion of the field many years ago. Cv. Russet Burbank is resistant to pathotype 1 which occurs in the United Kingdom, but is susceptible to pathotype 2, which predominates in Newfoundland (1). A soil survey confirmed the presence of S. endobioticum resting sporangia in the 1-ha area in which the symptomatic tubers were found. Concentrations of sporangia ranged from <1 to 124 sporangium per g of air-dried soil. Resting sporangia of S. endobioticum were not found in soil samples from fields within a 0.8-km radius of the infested field, nearby garden plots, or fields in which the same equipment had been used since 1984. References: (1) M. C. Hampson. Can. J. Plant Pathol. 15:223, 1993. (2) M. L. Putnam and A. B. Sindermann. Am. Potato J. 71:743, 1994.
First Report of Pepino mosaic virus on Tomato in Spain
Authors: Jordá C. , Pérez A. Lázaro , Martínez-Culebras P. , Abad P. , Lacasa A. , Guerrero M. M. 
Published: May 2007

At the beginning of 2000, a damaging disease developed on protected tomato (Lycopersicon esculentum) crops grown in polyethylene greenhouses in different regions of Spain. Production losses were estimated at 15 to 80%. The tomato plants showed a variety of symptoms. The most common symptoms were leaf distortion, chlorosis, and mosaic. Some plants showed a dark green mosaic and bubbling of the leaf surface. Green striations were also observed on the stem and sepals. Most of the diseased plants had discolored fruits. Symptoms decreased as environmental temperature increased. The involvement of Pepino mosaic virus (PepMV) was suspected. To identify the etiological agent, ≈500 symptomatic tomato plants were collected from several locations in Alicante, Murcia, Almeria and the Canary Islands. Flexuous viral particles 510 nm long were observed by transmission electron microscopy, suggesting the presence of a potexvirus in the tissue extracts analyzed. All samples were tested by ELISA (enzyme-linked immunosorbent assay), using polyclonal antibodies to Narcissus mosaic virus (Adgen, Auchincriuve, Scotland), a virus serologically related to PepMV, and two antisera specific to PepMV (Adgen, Scotland and DMSZ, Braunschweig, Germany). PepMV was detected in 35% of the samples. Like PepMV, the virus infected (as confirmed by ELISA) greenhouse-grown Datura stramonium, Nicandra physalodes, Nicotiana benthamiana, N. clevelandii, Solanum tuberosum, and Vigna sinensis and did not infect Capsicum anuum, Cucumis sativus, Chenopodium amaranticolor, C. quinoa, Petunia × hybrida, Phaseolus vulgaris, Physalis floridana, N. glutinosa, N. rustica, or N. tabacum. The virus did infect Gomphrena globosa, which normally is not infected by PepMV. The first report of PepMV was on pepino (Solanum muricatum) in Peru in 1974 (1), but this virus has been recently reported in the Netherlands, England, Germany, and France on protected tomato crops (2). To our knowledge, this is the first report of PepMV in Spain, including the Canary Islands. References: (1) R. A. C. Jones et al. Ann. Appl. Biol. 94:61, 1980. (2) European and Mediterranean Plant Protection Organisation (EPPO). Alert List Viruses. On-line publication/2000/003.
Molecular Identification and Classification of a Phytoplasma Associated with Phyllody of Strawberry Fruit in Maryland
Authors: Jomantiene R. , Maas J. L. , Davis R. E. , Dally E. L. 
Published: May 2007

Several phytoplasmas have been reported to be associated with phyllody of strawberry fruit, including clover yellow edge, clover proliferation, clover phyllody, eastern and western aster yellows, STRAWB2, strawberry multicipita, and Mexican periwinkle virescence phytoplasmas. Plant symptoms in addition to phyllody may include chlorosis, virescence, stunting, or crown proliferation. In this report we describe a new phytoplasma in association with strawberry leafy fruit (SLF) disease in Maryland. Diseased plants exhibited fruit phyllody, floral virescence, leaf chlorosis, and plant stunting. Phytoplasmal 16S rDNA was amplified from SLF diseased plants by using the polymerase chain reaction (PCR) primed by primer pair P1/P7 and was reamplified in nested PCR primed by primer pair R16F2n/R2 (F2n/R2) as previously described (1). These results indicated the presence of a phytoplasma, designated SLF phytoplasma. Identification of SLF phytoplasma was accomplished by restriction fragment length polymorphism (RFLP) analysis of DNA amplified in PCR primed by F2n/R2, using endonuclease enzyme digestion with AluI, HhaI, KpnI, HaeIII, MseI, HpaII, RsaI, and Sau3AI. Phytoplasma classification was done according to the system of Lee et al. (2). RFLP analyses of rDNA amplified in three separate PCRs gave identical patterns. On the basis of collective RFLP patterns of the amplified 16S rDNA, the SLF phytoplasma was classified as a member of group 16SrIII (group III, X-disease phytoplasma group). The HhaI RFLP pattern of SLF 16S rDNA differed from that of the apparently close relative, clover yellow edge (CYE) phytoplasma, and all other phytoplasmas previously described in group III. Based on these results, SLF phytoplasma was classified in a new subgroup, designated subgroup K (III-K), within group III. The 1.2 kbp DNA product of PCR primed by primer pair F2n/R2 was sequenced, and the sequence deposited in GenBank under Accession No. AF 274876. Results from putative restriction site analysis of the sequence were in agreement with the results from actual enzymatic RFLP analysis of rDNA amplified from phylloid strawberry fruit. Although the sequence similarity between the 1.2-kbp fragment from the 16S rDNA of SLF phytoplasma and that of CYE phytoplasma was 99.9%, the Hha1 RFLP pattern of SLF rDNA supports the conclusion that the SLF phytoplasma may be closely related to, but is distinct from, CYE and other strains that are classified in group III. These findings contribute knowledge about the diversity of phytoplasmas affiliated with group III and the diversity of phytoplasmas associated with diseases in strawberry. References: (1) R. Jomantiene et al. Int. J. Syst. Bacteriol. 48:269, 1998. (2) I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998.
First Report of Tomato Spotted Wilt Virus on Potatoes in Iran
Authors: Pourrahim R. , Farzadfar Sh. , Moini A. A. , Shahraeen N. , Ahoonmanesh A. 
Published: May 2007

Severe leaf and stem necrosis before flowering was observed in potato (Solanum tuberosum) fields of Firouzkoh Province, Iran, during the summer of 1998. Infected plants died before the end of the growing season. Necrosis was more severe in cv. Agria than in cvs. Ajaxs and Arinda. A high population of Thrips tabaci was observed in August and September. Tomato spotted wilt virus (TSWV) (1) was detected in affected potatoes by using specific TSWV-IgG (from Bioreba) in double-antibody sandwich enzyme linked immunosorbent assay and by indicator plant reactions. Mechanical inoculation of indicator plants with leaf extracts of symptomatic potatoes produce necrotic local lesions in Chenopodium quinoa, C. amaranticolor, Gomphrena globosa, Vicia faba, Vigna sinensis, Phaseolus aureus var. Gohar, P. vulgaris, and Petunia hybrida. The virus caused systemic necrosis in Capsicum frutescens, Datura stramonium, D. metel, Nicotiana glutinosa, N. rustica, and Trapaeolum majus, preceded by systemic chlorotic spots. TSWV was reported from ornamental crops in Tehran and Absard areas near to Firouzkoh province (2), but this is the first report of TSWV occurrence on potatoes in Iran. References: (1) T. S. Ie. Descriptions of Plant Viruses. No. 39, 1970. (2) A. A. Moeini, et al. Iran. J. Plant Pathol. (In press.)
Outbreaks of Soybean Frogeye Leaf Spot in Iowa
Authors: Yang X. B. , Uphoff M. D. , Sanogo S. 
Published: May 2007

Frogeye leaf spot of soybean, caused by Cercospora sojina, is typically a disease of warm and humid regions (2). Although the disease was reported in the Midwest in the 1920s (1), no outbreaks have been recorded in Iowa. Outbreaks of frogeye leaf spot occurred during 1999 in soybean fields in Ames and Grand Junction in central Iowa. During the 2000 growing season, the disease occurred in southwestern, southcentral, central, southeastern, and east-central Iowa. Occurrences of the disease with severity (reduction of green leaf area) greater than 50% were observed in production soybean fields at Grand Junction in central Iowa and Central City in eastern Iowa. In a 12-ha no-till field planted with cv. Asgrow 2501, the disease was noticeable and uniformly distributed in the entire field in mid July. Disease severity in this field was greater than 70% by the end of August. Disease incidence, however, was less than 10% in three adjacent soybean fields. In a soybean performance test at a central Iowa location where the disease occurred in 1999 and 2000, the disease was observed on all 80 varieties, with four having a severity equal to or greater than 40%. Fourteen entries had less than a 10% disease severity and 19 entries had a disease severity equal to or greater than 30%. Infected leaves in these locations had typical lesions of frogeye leaf spot, which appeared as reddish brown margins surrounding light brown or ash gray centers. On the infected tissues, hyaline, straight, and multiseptate conidia from clustered conidiophores were found, isolated, and identified to C. sojina. The relatively warm winter temperatures in 1998 to 1999 and 1999 to 2000 were associated with frogeye leaf spot epidemics. Because of the seedborne nature of C. sojina, efforts are warranted to monitor and survey the occurrence of frogeye leaf spot in Iowa, an important seed production state in the northern soybean production region. References: (1) K. Athow and A. H. Probst. Phytopathology 42:660–662, 1952. (2) D. V. Phillips. 1999. Pages 20–21 in: Soybean Disease Compendium. Hartman et al. eds, American Phytopathological Society. St. Paul, MN.
First Report of an Aster Yellows Phytoplasma Associated with Cabbage in Southern Texas
Authors: Lee I.-M. , Dane R. A. , Black M. C. , Troxclair Noel 
Published: May 2007

In early spring 2000 carrot crops in southwestern Texas were severely infected by an outbreak of phyllody associated with aster yellows phytoplasma. Cabbage crops that had been planted adjacent to these carrot fields began to display previously unobserved symptoms characteristic of phytoplasma infection. Symptoms included purple discoloration in leaf veins and at the outer edges of leaves on cabbage heads. Proliferation of sprouts also occurred at the base of the stem and between leaf layers of some plants, and sprouts sometimes continued to proliferate on extended stems. About 5% of cabbage plants in the field exhibited these symptoms. Two symptomless and four symptomatic cabbage heads were collected in early April from one cabbage field. Veinal tissues were stripped from each sample and used for total nucleic acid extraction. To obtain specific and sufficient amount of PCR products for analysis, nested PCR was performed by using primer pairs (first with P1/P7 followed by R16F2n/R16R2) (1,2) universal for phytoplasma detection. A specific 16S rDNA fragment (about 1.2 kb) was strongly amplified from the four symptomatic but not from the two asymptomatic samples. The nested PCR products obtained from the four symptomatic samples were then analyzed by restriction fragment length polymorphism (RFLP) using the restriction enzymes MseI, HhaI, and HpaII, and the RFLP patterns were compared to the published patterns of known phytoplasmas (1). The resulting RFLP patterns were identical to those of a phytoplasma belonging to subgroup B of the aster yellows phytoplasma group (16SrI). These RFLP patterns were also evident in putative restriction sites observed in a 1.5 kbp nucleotide sequence of the 16S rDNA. This is the first report of aster yellows phytoplasma associated disease symptoms in cabbage in Texas. The occurrence of cabbage proliferation coincided with the presence of high populations of the insect vector, aster leafhopper. References: (1) I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998. (2) B. Schneider et al. 1995. Molecular and Diagnostic Procedures in Mycoplasmology, Vol. I. Academic Press, San Diego, CA.
First Report of Cabbage leaf curl virus (Family Geminiviridae) in Georgia
Authors: Mandal B. , Langston D. B. , Pappu H. R. , Beard G. H. , Kucharek T. A. , Flanders J. T. , Whiddon J. P. , Smith J. E. , Kelley W. T. 
Published: May 2007

Cabbage and collard greens were inflicted with a previously undescribed virus-like disease during the fall 2000. Symptoms on leaves were yellow spots, vein clearing, mosaic, curling, and puckering. Symptomatic plants were widespread in Brooks, Colquitt, Grady, and Pierce counties in Georgia. Disease incidence ranged from 10 to 20% in the majority of the fields surveyed but some fields had 100% incidence. Fields were heavily infested by Bemisia argentifolii and the symptoms were suggestive of a whitefly-transmitted geminivirus infection. A polymerase chain reaction (PCR)-based diagnostic test for geminivirus was conducted. Total DNA was extracted from symptomatic cabbage and collard green plants collected from commercial fields. The two primers, 5'-GCCCACATYGTCTTYCCNGT-3' and 5'- GGCTTYCTRTACATRGG-3' (2,3), are “universal” for genus Begomovirus of family Geminiviridae. The primer pair could amplify a part of the replicase-associated protein and coat protein and the complete common region of DNA-A. The PCR gave a DNA band of expected size (1.1 kb) from both symptomatic cabbage and collard green samples, whereas no such product was obtained from healthy samples, suggesting that the causal agent could be a geminivirus. To establish the identity of the virus, the 1.1 kb PCR product was cloned into pGEM-T Easy (Promega) and sequenced. GenBank search showed that the geminivirus isolated in Georgia was most closely related (98% sequence identity) to Cabbage leaf curl virus (accession number U65529) reported from Florida (1). The virus was mechanically transmitted to healthy cabbage and collard green plants under experimental conditions. To our knowledge, this is the first report of Cabbage leaf curl virus from Georgia. References: (1) A. M. Abouzid et al. Phytopathology 82:1070, 1992. (2) S. S. Pappu et al. Plant Dis. 84:370, 2000. (3) M. R. Rojas et al. Plant Dis. 77:340–347, 1993.