bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fibroblast activation protein as a biomarker for containment of triple-negative breast cancer
Authors: Chen Yuanyuan , Li Xiaojun , Zhang Wei 
Published: April 2024

Abstract

Objectives
The aim of this study was to investigate effect of Talabostat (a fibroblast activation protein inhibitor) on the cell viability of MDA-MB-231 cells and organoids. And the mechanism of containment of cells and organoids was also studied.
Methods
Cell viability of cells was demonstrated through some assays, including CCK-8, Transwell, etc. Potential mechanisms were investigated through TEM, TUNEL staining, western blot and flow cytometry.
Results
It was found that the cell viability and migration of MDA-MB-231 cells were inhibited by Talabostat (one kind of FAP inhibitor). And the mitochondria-mediated apoptosis induced by Talabostat was considered as the proliferation suppression mechanism, which was certified by RNA sequencing results. More importantly, the proliferation inhibitions of organoids induced by Talabostat were realized, which suggests the repression of FAP for TNBC treatment has strong clinical values.
Conclusions
Herein, the therapy effect of Talabostat on triple-negative breast cancer was investigated systematically, and it was found that the cell viability and migration of MDA-MB-231 cells were inhibited by Talabostat. And the mitochondria-mediated apoptosis induced by Talabostat was considered as the proliferation suppression mechanism, which was certified by RNA sequencing results. Importantly, the proliferation inhibitions of organoids induced by Talabostat were realized, which suggests the repression of FAP for TNBC treatment has strong clinical values. Our findings shed new lights on the promising treatment strategies for TNBC.
The incidence of delirium in the elderly after surgery and risk factors
Authors: Noh Nebal , Ali Diaa Haj , Rakmani Najwa 
Published: April 2024

Abstract
Background
Delirium is a sudden, fluctuating, and usually curable disorder of mental function that is characterized by an inability to pay attention, disorientation, an inability to think clearly, and fluctuations in the level of alertness (consciousness). Recognition and treatment of delirium are critical because postoperative delirium is associated with outcomes including functional deterioration, longer hospitalizations, increased Costs, and high mortality rates.
Research Materials and Methods
A retrospective cohort study (COHORT) was conducted On a random group of elderly patients after surgery from December 2023 to April. A paper questionnaire was conducted and patients visiting the General Surgery Department in Damascus Hospital were followed up during the period of conducting the research. Accordingly, the questionnaire was filled out and the data was reviewed under the supervision of the research supervisor.
The final sample size was 70 participants
Results
The sample included 70 patients, of which 6 were excluded, leaving us with 64 cases on which research and statistics were conducted. 57.8% were females and 42.2% were males. Participants are classified into three groups, with the age group (70 – 79) years representing the majority 71.8% (mean 76 years). 65.6% of patients did not suffer from delirium after surgery and Inability to pay attention was the most common Symptoms, these symptoms appeared during the first week at 54.5%. regarding the treatment of delirium, 90.9% of patients were treated by treating the cause, and according to the outcome of the delirium, 36.4% have completely recovered.
Conclusion
It is necessary to hold medical seminars on delirium and explain the difference between its types, as well as publish awareness campaigns on the necessity of educating medical staff, especially students, doctors, and nurses, about the psychological consequences of surgery and ways to deal with them.
THE INFLUENCE OF FULL-SCALE MILITARY AGGRESSION OF RUSSIA ON REGISTRATION OF CIVIL STATUS ACTS
Authors: Hrytsenko Halyna H. 
Published: April 2024

The relevance of this article is explained by the need to analyze the functioning of civil registration authorities in the context of armed conflicts throughout the territory of Ukraine. The study of this topic has gained particular importance in light of the realities of military actions and Russia's aggression in Ukraine. An analysis of scientific literature and legislation allows us to conclude that, despite significant challenges, the full-scale military aggression by Russia did not lead to the collapse of the system of civil registration of acts of civil status and the judiciary in Ukraine. The government has taken effective measures to ensure the functioning of these authorities in the conditions of armed conflict. For instance, the principle of extraterritoriality was applied during the state of war, simplifying the procedure for registering acts of civil status. In many cases, the payment of state fees and court fees for events such as marriages and the establishment of facts of birth and death in areas affected by military operations was waived. It is important to note that the protection of the lives and health of citizens during military actions was ensured. For example, servicemen and healthcare institution employees had the opportunity to register marriages without personal presence, and police officers conducted examinations only on the bodies where signs of violent death were present. Changes to the procedure for registering the
death of individuals are thoroughly analyzed. This article examines crucial aspects of the functioning of the civil registration system in the context of armed conflict and emphasizes the measures taken to ensure the stability and convenience of citizens during this challenging period, with a focus on the peculiarities of registering acts of civil status in temporarily occupied territories.
Direct injection ultra-performance liquid chromatography–tandem mass spectrometry for the high-throughput determination of etomidate and etomidate acid in wastewater
Authors: Yuan Shuai , Xiao Yue , Luo Ruxin , Wang Xin , Xiang Ping 
Published: April 2024

ABSTRACT
Etomidate (ET), a hypnotic agent used for the induction of anesthesia, is rapidly metabolized to etomidate acid (ETA) in the liver. Recently, ET has become one of the most serious alternative drugs of abuse in China. Therefore, an urgent need exists to develop a fast and convenient analysis method for monitoring ET. The current work presents a simple, fast, and sensitive direct injection method for the determination of ET and ETA in wastewater. After the optimization of the ultra-performance liquid chromatography–tandem mass spectrometry and sample filtration conditions, the method exhibited satisfactory limits of detection (1 ng/L) and good filtration loss. The validated method was successfully applied to determine the concentrations of ET and ETA in wastewater samples (n = 245) from several wastewater treatment plants in China. The concentrations of the targets in positive samples ranged from less than the lower limits of quantitation to 47.71 ng/L. The method can meet ET monitoring and high-throughput analysis requirements.
Influence of the Use of a Mixed Solution of Equal Amounts of Amyl Acetate, Acetone, and Ethanol on the Cleaning of Endodontic Sealer Residues on the Bond Strength of the Fiber Post Cementation System: A Laboratory Investigation
Authors: Oliveira Barros Antonia Patricia , Freitas Ana Paula Aparecida Raimundo Alves , Kokol Frederico Guilherme Otto , de Souza Elizangela Maria Pereira , Junior Adirson Jorge , de Melo Alencar Cristiane , de Andrade Marcelo Ferrarezi , Kuga Milton Carlos 
Published: April 2024

Purpose
The study aimed to evaluate the effect of different chemical solutions (ET, 95% ethanol, and ES, experimental solution of amyl acetate, acetone, and 95% ethanol) on the removal of epoxy-based sealer residues (AH, AH Plus Jet, or AD, Adseal) from the adhesive interface between root dentin and universal adhesive in post space third, after 6 months control.

Methods
Eighty bovine roots were prepared and randomly divided according to the sealer and cleaning solution: AH Plus Jet + 95% ethanol (AH+ET), Adseal + 95% ethanol (AD+ET), AH Plus Jet + experimental solution (AH+ES), Adseal + experimental solution (AD+ES). Scanning electron microscopy was used to assess the incidence of residue in the post space, and the data obtained were subjected to Kruskal-Wallis/Dunn tests (α=0.05). Bond strength was evaluated in push-out tests, and the data were subjected to ANOVA/Tukey analysis. The adhesive failure pattern was assessed using stereomicroscopy, and the data have been evaluated through incidence frequency.

Results
AH+ES and AD+ES showed lower residues incidence and higher bond strength values (p<0.05). Type 4 and 3 failures were observed in the cervical and middle thirds in the groups AH+ET, AD+ET, and AH+ES, AD+ES, respectively.

Conclusion
The experimental solution positively influenced the bond strength of the post space.
Exploring the Painless Nature and Potential Mechanisms of Asymptomatic Irreversible Pulpitis: A Narrative Review
Authors: Cabrera-Abad Daniela Paola , Jara-Vergara Verónica Cristina , Álvarez-Vásquez José Luis 
Published: April 2024

Background
Considering the extensive innervation of the pulp tissue, asymptomatic irreversible pulpitis (AIP) or “silent pulpitis” represents a confounding clinical condition. Previous studies have attributed the painless nature of AIP to the inhibition of pulpal nociceptors by local endogenous analgesics. However, there is a lack of recent information concerning its painless nature, and paradoxically, patients with dental pain are diagnosed with AIP daily worldwide. In addition, no recent review has explored the potential AIP-related mechanisms.

Objective
This narrative review aims to explore and update the potential mechanisms involved in the painless nature of AIP to improve our current understanding of the asymptomatic character of this clinical condition.

Methods
An electronic search was performed in the PubMed and Scopus databases, using as search terms “asymptomatic irreversible pulpitis,” “dental pulp,” “endogenous opioids,” “endogenous cannabinoids,” “somatostatin,” “GABA,” “bombesin,” “cortistatin,” “galanin,” and “specialized pro-resolving lipid mediators.”

Results
Endogenous opioids, G protein-activated inwardly rectifying K+ channels, endogenous cannabinoids, γ-aminobutyric acid, and neuropeptides (i.e. somatostatin, cortistatin, galanin, and bombesin) could be involved in AIP-related analgesia. Additionally, specialized pro-resolving lipid mediators, such as lipoxins, resolvins, maresins, and protectins, as well as oxytocin, phoenixin, opiorphin, and adipokines, could also be involved in this clinical condition.

Conclusion
This narrative review provides updated information on the potentially involved mechanisms in AIP. Nevertheless, the precise mechanisms responsible for the lack of symptoms in AIP remain to be elucidated, and further research is warranted.
Intelligent Plant Leaf Disease Detection Using Generative Adversarial Networks: a Case-study of Cassava Leaves
Authors: Lokesh Gururaj Harinahalli , Chandregowda Soundarya Bidare , Vishwanath Janhavi , Ravi Vinayakumar , Ravi Pradeep , Al Mazroa Alanoud 
Published: April 2024

Background
Cassava leaf disease detection is a major problem since it is very difficult to identify the disease in naked-eye observation and even experts such as agricultural scientists can fail in this task. The approach we use in this paper has the potential to overcome this problem.

Materials and Methods
In this, we propose an approach based on artificial intelligence for leaf disease detection using deep learning with generative adversarial networks (GAN). Our experimental study used a dataset including 12880 cassava leaf pictures generated using CycleGAN showing five major disease classes. In order to avoid overfitting, a GAN architecture is proposed for data augmentation using two networks, i.e., a Generator and a Discriminator. The generator is trained to generate similar data samples as the original data

Results
The proposed approach achieved an accuracy of 99.51% for the classification of healthy or unhealthy leaf images, which outperformed existing methods.

Discussion
The discriminator is trained to distinguish between the unique and generated sample records, as actual or fake. To classify cassava images into five categories of diseases, a combination of machine learning models has been trained on original and generated images. The proposed approach showed better accuracy compared to the existing methods.

Conclusion
The proposed deep learning-based method can be used as a tool for early disease diagnosis in cassava leaf disease detection and classification
Insights into Alopecia Areata: A Systematic Review of Prevalence, Pathogenesis, and Psychological Consequences
Authors: Bahashwan Emad , Alshehri Mohja 
Published: April 2024

Background
Alopecia areata is an autoimmune condition distinguished by visual non-scarring hair loss in different head and face regions. The condition affects every age group and is prevalent in all types. Alopecia areata is a disorder that arises from a malfunction of the immune prerogative of the hair follicle, which can be managed with new treatments and remission.

Objective
This study aimed to determine the psychological impacts of Alopecia, the frequency of the condition, and its impact on quality of life.

Methods
Data from relevant studies conducted between 2018 and 2023 were collected using the PRISMA criteria and the SPIDER framework. For this review, 15 studies were selected using specific keywords, such as Alopecia Areata, Alopecia Prevalence, Alopecia areata management, and Alopecia areata treatment on 8 search engines, such as PubMed, Scopus, Google Scholar and more. The papers were selected through inclusion and exclusion criteria. Psychological factors, such as mental health, anxiety, stress and depression, were found to play a significant role in this autoimmune condition.

Results
The updated approach in this study analyzes the prevalence of Alopecia areata,its impacts on daily life, and underlying mechanisms. It emphasizes the psychological burdens and advocates for awareness, education, and support for affected individuals.

Conclusion
This systematic review advances the understanding of the prevalence of Alopecia areata and its psychological effects, with potential implications for healthcare practices and policy decisions.
The Effect of Socioeconomic Drivers on Under-five Mortality Rates: A Survey Bayesian Meta-analysis Study
Authors: Dlamini Welcome J. , Melesse Sileshi F. , Mwambi Henry G. 
Published: April 2024

Background
Studies on approaches for combining information from related studies have been well-documented in the literature. However, limited research has been conducted to focus on the issue of combining parameter estimates in the context of under-five mortality.

Objectives
The objective of this study was to study the overall effect of socioeconomic factors on under-five mortality, considering the censoring problem and survey design features.

Methods
This study estimates the overall effect of risk factors on under-five mortality in four countries from the sub-Saharan African region using Bayesian hierarchical meta-analysis. The data used in the study is from the previous four demographics and health surveys for a research area.

Results
The results obtained using the Bayesian Meta Cox PH model are almost similar to those using the extended Cox except for one key finding. A child from a rural area has an increased risk of dying compared to a child from an urban area. Whereas it is insignificant when using the extended Cox model.

Conclusion
The study has demonstrated drivers of child mortality using Bayesian hierarchical meta-analysis.
Beyond Patient Safety Goal Towards Hospital Sustainable Risk: A Systematic Review on the Evolution of Hospital Risk Management
Authors: Sae-Lim Patipan , Na Ayudhaya Sirintata (Pongpech) Singhara 
Published: April 2024

Background
Risk management is an indispensable tool in every business sector, yet the aspects of it are various given the business types. The hospital industry employs risk management as a day-to-day operation, but most of the time, risk management to them is related to only patient safety. In this study, our team hypothesized if there are any other aspects of hospital risk management from the research landscape.

Objective
In this manuscript, we study the aspects of hospital risk management from bibliometric data to explore hospital risk research trends.

Methods
We explore the hospital risk management research landscape to analyze past, present and future research trends using quantitative bibliometric analysis. Bibliometric data were extracted from the SCOPUS database since 1980. Descriptive analysis relating to research landscape performance will be explored. Additionally, co-occurrence analysis clustering by author’s keywords will be analyzed.

Results & Discussion
Our team extracted 803 documents related to hospital risk management from SCOPUS. The publications in this field are increasing from time to time with a 7.97% annual rate. The most productive country studying hospital risk management is the USA, while the most productive authors and citations focus on the studying of patient safety and clinical risk. However, analysing author keywords using co-occurrence, clustering, as well as a thematic map can gain hidden insights that there are emerging themes of other aspects of hospital risk management, which are: enterprise risk management and sustainability. However, these studies are researched marginally compared to clinical risk management.

Conclusion
Although studying hospital risk management has constantly changed for 43 years, the main focus is still clinical risk and patient safety. However, a successful hospital has the ability to enhance all stakeholders, not only from the patient’s aspect. Studying enterprise risk management and sustainability in hospitals is thus an important trend in the future.
Application of Simulation Technology in Football Training: A Systematic Review of Empirical Studies
Authors: Jia Teng , Sitthiworachart Jirarat , Morris John 
Published: April 2024

Background
This review aimed to cover the characteristics and functions of simulation tools applied to football training, the process and results of empirical research, the benefits of simulation tools for football training, and existing challenges.

Materials and Methods
To investigate and analyze the effect of simulation technology in football training, the PRISMA method was used to systematically review 18 relevant empirical studies published between January, 2014 and July, 2023.

Results
The study identified three types of tools for applying simulation technology to football training, including head-mounted displays, Cave Automatic Virtual Environment (CAVE), and Screen-Based Simulation. These tools have been effective in training football playing techniques (including goalkeeping, heading, etc.) as well as football tactical skills (including perception-cognitive and decision-making) and can be used as a supplement to regular training.

Conclusion
If simulation technology is to enhance football training, we suggest that it is necessary to carefully verify the validity of the tool and the long-term impact of simulation training on participants and verify that simulation actually translates to real-world games. At the same time, it is suggested that future research could explore training with mixed VR and AR to develop more realistic and effective training platforms.
Knowledge Transfer and Networking Upon Implementation of a Transdisciplinary Digital Health Curriculum in a Unique Digital Health Training Culture: Prospective Analysis
Authors: Kröplin Juliane , Maier Leonie , Lenz Jan-Hendrik , Romeike Bernd 
Published: April 2024

Abstract

Background
Digital health has been taught at medical faculties for a few years. However, in general, the teaching of digital competencies in medical education and training is still underrepresented.

Objective
This study aims to analyze the objective acquisition of digital competencies through the implementation of a transdisciplinary digital health curriculum as a compulsory elective subject at a German university. The main subject areas of digital leadership and management, digital learning and didactics, digital communication, robotics, and generative artificial intelligence were developed and taught in a transdisciplinary manner over a period of 1 semester.

Methods
The participants evaluated the relevant content of the curriculum regarding the competencies already taught in advance during the study, using a Likert scale. The participants’ increase in digital competencies were examined with a pre-post test consisting of 12 questions. Statistical analysis was performed using an unpaired 2-tailed Student t test. A P value of <.05 was considered statistically significant. Furthermore, an analysis of the acceptance of the transdisciplinary approach as well as the application of an alternative examination method (term paper instead of a test with closed and open questions) was carried out.

Results
In the first year after the introduction of the compulsory elective subject, students of human medicine (n=15), dentistry (n=3), and medical biotechnology (n=2) participated in the curriculum. In total, 13 participants were women (7 men), and 61.1% (n=11) of the participants in human medicine and dentistry were in the preclinical study stage (clinical: n=7, 38.9%). All the aforementioned learning objectives were largely absent in all study sections (preclinical: mean 4.2; clinical: mean 4.4; P=.02). The pre-post test comparison revealed a significant increase of 106% in knowledge (P<.001) among the participants.

Conclusions
The transdisciplinary teaching of a digital health curriculum, including digital teaching methods, considers perspectives and skills from different disciplines. Our new curriculum facilitates an objective increase in knowledge regarding the complex challenges of the digital transformation of our health care system. Of the 16 student term papers arising from the course, robotics and artificial intelligence attracted the most interest, accounting for 9 of the submissions.