bluuebunny/crossref_metadata_2025_split huggingface dataset:
NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization
Authors: Zou Shaomin , Huang Yizhi , Yang Ziqing , Zhang Jieping , Meng Manqi , Zhang Yijing , Feng Junyan , Sun Rui , Li Weiyao , Wang Wencong , López Jesús García‐Foncillas , Fang Lekun 
Published: March 2024

AbstractBackgroundNOP2/Sun domain 2 (NSUN2) is one of the important RNA methyltransferases catalyzing 5‐methylcytosine (m5C) formation and participates in many critical bioprocesses. However, the roles and underlying molecular mechanisms of NSUN2‐mediated m5C modification in colorectal cancer (CRC) remain unclear.MethodsTo explore the NSUN2 expression in CRC, fresh tissue samples were collected and Nsun2 knockout mouse was constructed. In vitro and in vivo functional assays were conducted to assess the role of NSUN2. RNA array and bisulfite sequencing were used to investigate the potential targets. The mechanisms of NSUN2 function on SKIL were identified by m5C‐methylated‐RNA immunoprecipitation and RNA stability assays. Additionally, tissue microarray analysis was conducted and patient‐derived tumour xenograft mouse (PDX) models were used to define the potential therapeutic targets.ResultsNSUN2 was highly expressed in CRC and correlated with poor CRC patient survival. Moreover, silencing NSUN2 suppressed CRC tumourigenesis and progression in Nsun2 knockout mouse models. In vitro and in vivo studies suggested that NSUN2 promoted colorectal cancer cell growth. Mechanistically, SKI‐like proto‐oncogene (SKIL) is positively regulated by NSUN2, and the NSUN2‐SKIL axis is clinically relevant to CRC. NSUN2 induced m5C modification of SKIL and stabilized its mRNA, which was mediated by Y‐box binding protein 1 (YBX1). Elevated SKIL levels increased transcriptional coactivator with PDZ‐binding motif (TAZ) activation.ConclusionsOur findings highlight the importance of NSUN2 in the initiation and progression of CRC via m5C‐YBX1‐dependent stabilization of the SKIL transcript, providing a promising targeted therapeutic strategy for CRC.
Simulation of reservoir dynamic damage and influencing factors of horizontal wells in heterogeneous carbonate reservoirs
Authors: Xu Shijing , Wang Guoqing , Gao Bin , Tian Jiaxin 
Published: March 2024

AbstractAs the development of well types for low permeability reservoirs shifts from vertical to highly inclined/horizontal wells, targeted studies of oil and gas reservoir damage must be conducted for such well types to improve the science and effectiveness of acidification conversion. Taking into account the formation of mud cake and the dynamic damage factors caused by drilling fluid on the reservoir during horizontal well drilling, a refined dynamic description model for nonuniform damage in horizontal wells was established. The coupled simulation accounts for the dynamical destruction of the skin factor of the reservoir and the dynamical generation of the slurry. The coupled model consists of a radial mud cake dynamic formation model, a reservoir damage radius model, a permeability distribution model, and a horizontal well inhomogeneous skin factor model. It captures the dynamic evolution of reservoir damage due to drilling fluid contamination during horizontal well drilling and provides a real‐time representation of changes in the reservoir skin factor of horizontal wells, measuring the depth and extent of radial reservoir damage. Clarifying the extent of reservoir damage and its impact in horizontal well sections can help select appropriate acidification methods, improve acid distribution processes, and ultimately improve the effectiveness of mitigating reservoir damage and increasing oil and gas well productivity.
IGF2BP2 and IGFBP3 Genotypes, Haplotypes, and Genetic Models Studies in Polycystic Ovary Syndrome
Authors: Govahi Kakhki Fatemeh , Sargazi Saman , Montazerifar Farzaneh , Majidpour Mahdi , Karajibani Atena , Karajibani Mansour , Ghasemi Marzieh 
Published: March 2024

ABSTRACTBackgroundInsulin resistance has been correlated with the genetic diversity within the insulin‐like binding proteins genes. Moreover, insulin resistance is one of the key characteristics of the widespread reproductive endocrine condition known as polycystic ovarian syndrome (PCOS). Hence, this study is aimed to determine the association between IGFBP3 and IGF2BP2 gene variants and PCOS risk.MethodsA total of 300 subjects (150 PCOS cases diagnosed based on Rotterdam ESHRE/ASRM consensus criteria and 150 healthy subjects) were recruited in this case–control cross‐sectional study. Tetra‐primer amplification refractory mutation system polymerase chain reaction (ARMS‐PCR) was used for genotyping rs11705701, whereas genotyping of rs1470579 and rs2854744 was done employing PCR‐restriction fragment length polymorphism (PCR‐RFLP) technique.ResultsThe CC and AA+AC genotypes of rs1470579 conferred an increased risk of PCOS in our population. Regarding the rs2854744, an increased risk of PCOS was observed under the codominant homozygous (TT vs. GG) model by 2.54 fold. The C allele of rs1470579 and T allele of rs2854744 enhanced PCOS risk by 1.97 and 1.46 folds, respectively. Haplotype analysis showed that the Ars1470579Ars11705701 haplotype conferred a decreased risk of PCOS (odds ratio = 0.53, 95% confidence interval = 0.34–0.83, p = 0.006). The AC/GG/GT, AA/GA/GT, AC/GA/GG, and AC/GA/GT genotype combinations of rs1470579/rs11705701/rs2854744 were associated with a decreased risk of the disease.ConclusionsIGF2BP2 rs1470579 and IGFBP3 rs2854744 enhanced PCOS susceptibility in a Southeastern Iranian population. Further investigation involving larger cohorts representing diverse ethnic backgrounds is needed to confirm the current findings.
Safety of intracranial electrodes in an MRI environment: a technical report
Authors: Bezchlibnyk Yarema B. , Quiles Rolando , Barber Jeremy , Osa Benjamin , Clifford Keven , Murtaugh Ryan 
Published: March 2024

AbstractIntroductionIntracranial electroencephalography (iEEG) involves placing intracranial electrodes to localise seizures in patients with medically refractory epilepsy. While magnetic resonance imaging (MRI) enables visualisation of electrodes within patient‐specific anatomy, the safety of these electrodes must be confirmed prior to routine clinical utilisation. Therefore, the purpose of this study was to evaluate the safety of iEEG electrodes from a particular manufacturer in a 3.0‐Tesla (3.0T) MRI environment.MethodsMeasurements of magnetically induced displacement force and torque were determined for each of the 10 test articles using standardised techniques. Test articles were subsequently evaluated for radiofrequency‐induced heating using a Perspex phantom in both open and ‘fault’ conditions. Additionally, we assessed radiofrequency (RF)‐induced heating with all test articles placed into the phantom simultaneously to simulate an implantation, again in both open and ‘fault’ conditions. Finally, each test article was evaluated for MRI artefacts.ResultsThe magnetically induced displacement force was found to be less than the force on the article due to gravity for all test articles. Similarly, the maximum magnetically induced torque was less than the worst‐case torque due to gravity for all test articles apart from the 8‐contact strip – for which it was 11% greater – and the depthalon cap. The maximum temperature change for any portion of any test article assessed individually was 1.7°C, or 1.2°C for any device component meant to be implanted intracranially. In the implantation configuration, the maximum recorded temperature change was 0.7°C.ConclusionsMRI may be safely performed for localising iEEG electrodes at 3.0T under certain conditions.
Kulla‐RIV: A composing model with integrity verification for efficient and reliable data processing services
Authors: Reyes‐Anastacio Hugo G. , Gonzalez‐Compeán Jose L. , Sosa‐Sosa Victor J. , Marcelín‐Jiménez Ricardo , Morales‐Sandoval Miguel 
Published: March 2024

AbstractThis article presents the design and implementation of a reliable computing virtual container‐based model with integrity verification for data processing strategies named the reliability and integrity verification (RIV) scheme. It has been integrated into a system construction model as well as existing workflow engines (e.g., Kulla and Makeflow) for composing in‐memory systems. In the RIV scheme, the reliability (R) component is in charge of providing an implicit fault tolerance mechanism for the processes of data acquisition and storage that take place in a data processing system. The integrity verification (IV) component is in charge of ensuring that data transmitted/received between two processing stages are correct and are not modified during the transmission process. To show the feasibility of using the RIV scheme, real‐world applications were created by using different distributed and parallel systems to solve use cases of satellite and medical imagery processing. This evaluation revealed encouraging results as some solutions that assumed the cost (overhead) of using the RIV scheme, for example, Kulla (the Kulla‐RIV solution), achieve better response times than others without the RIV scheme (e.g., Makeflow) that remain exposed to the risks caused by to the lack of RIV strategies.
Protective effect of crocin on peroxidation‐induced oxidative stress and apoptosis in IPEC‐J2 cells
Authors: Wang Jing , Song Hongbing , Huang Yongjie , Yang Chu , Wu Yanling , Lin Ruiyi , Xiao Tianfang , Lin Weimin 
Published: March 2024

AbstractThe antioxidant properties of crocin are attracting interest, yet the underlying mechanisms by which crocin mitigates oxidative stress‐induced intestinal damage have not been determined. This study aimed to elucidate the effects of crocin on oxidative stress, apoptosis, and intestinal epithelial injury in intestinal epithelial cells (IPEC‐J2). Using an H2O2‐induced oxidative stress model in IPEC‐J2 cells, crocin was added to assess its effects. Cell viability and apoptosis were evaluated using methyl thiazolyl tetrazolium assays and flow cytometry. Additionally, oxidative stress markers, such as superoxide dismutase (SOD), catalase (CAT), reactive oxygen species (ROS), and malondialdehyde (MDA), were quantified. We investigated, in which cell oxidation and apoptosis were measured at the gene and protein levels and employed transcriptome analysis to probe the mechanism of action and validate relevant pathways. The results showed that crocin ameliorates H2O2‐induced oxidative stress by reducing ROS and MDA levels and by countering the reductions in CAT, total antioxidant capacity, and SOD. Crocin also attenuates the upregulation of key targets in the Nrf2 pathway. Furthermore, it effectively mitigated IPEC‐J2 cell apoptosis caused by oxidative stress, as evidenced by changes in cell cycle factor expression, apoptosis rate, mitochondrial membrane potential, and apoptosis pathway activity. In addition, crocin preserves the integrity of the intestinal barrier by protecting tight junction proteins against oxidative stress. Transcriptome sequencing analysis suggested that the mitochondrial pathway may be a crucial mechanism through which crocin exerts its protective effects. In summary, crocin decreases oxidative stress molecule formation, inhibits Nrf2 pathway activity, prevents apoptosis‐induced damage, enhances oxidative stress resistance in IPEC‐J2 cells, and maintains redox balance in the pig intestine.
Thermal polymerization induced phase separation of poly(methyl methacrylate)/poly(2‐hydroxyethyl methacrylate) blend for soft optical materials
Authors: Van‐Pham Dan‐Thuy , Bui‐Thi Ngoc‐Han , Tran‐Cong‐Miyata Qui 
Published: March 2024

AbstractIn this study, phase separation induced by free radical polymerization of 2‐hydroxyethyl methacrylate (HEMA) and poly(methyl methacrylate) (PMMA) with various compositions in term of weight percentage was investigated. Particularly, experiments were carried out at different temperatures from 70 to 110 °C and weight content of PMMA from 1 to 15 wt.%. The effects of these parameters on morphology and properties of the polymer blends were observed and analyzed. Optical microscopy, X‐ray CT scanning were employed to observe the structure of the obtained blends. Based on the images of the obtained blends, the characteristic length and contrast value of the morphology were determined. In addition, optical transmittance characterization and thermal stability analysis of the products were performed by using UV‐Vis spectrometry and thermogravimetric analysis, respectively. It was found that the optimum blend that possessed 12 wt.% PMMA content and conducted at 100 °C allowed visual light transmittance of larger than 95 %. The results showed promising applicability of the polymer blend as an alternative for soft optically transparent materials.
Influence of palm oil modified epoxy resin/biscycloaliphatic diepoxide weight ratio on photoinitiated cationic crosslinking and properties of UV‐cured coatings
Authors: Thanh Do Minh , Hien Le Xuan , Thuong Nguyen Thi , Hung Trieu Quy , Son Lam Hung 
Published: March 2024

AbstractEffect of the weight proportion between epoxy resin modified by palm oil (EPO) and a biscycloaliphatic diepoxide (BCDE) on photoinitiated cationic crosslinking of the system containing EPO, BCDE, triarylsulfonium hexafluoroantimonate salts (TAS) as well as performance of formed coatings have been investigated. The change of TAS and functional groups of the system upon UV‐irradiation time was determined quantitatively by IR‐spectrometric analysis, using internal standard method. Formation of tridimensional polymer network in the coatings upon UV‐exposure was proved directly by evaluation of their gel fraction. It was demonstrated that epoxy group conversion and EPO/BCDE weight ratio relationship had optimal characteristic with the best consumptions of epoxy groups 5.94 and 6.13 mol/kg at 1.2 and 14.4 s of UV‐exposure, correspondingly, when EPO/BCDE weight ratio was 20/80. Augmentation of the EPO/BCDE ratio from 20/80 to 60/40 increased flexibility of UV‐cured coatings from 10 to 1 mm but decreased their gel fraction from 81 to 59 %; the relative hardness of the cured coatings diminished from 0.95 to 0.43 when the EPO/BCDE ratio varied from 10/80 to 40/60.
Chemical constituents of Albizia myriophylla wood and the HPLC determination of some high yield compounds as markers
Authors: Tam Pham Thi , Van Trung Phung , Nga Vo Thi , Tuyet Nguyen Thi Anh , Phung Nguyen Kim Phi , Duong Ngo Thi Thuy , Thu Nguyen Thi Hoai 
Published: March 2024

AbstractSpinasterol (1) and four flavonoids (2‐5) were isolated from Albizia myriophylla wood collected at Phu Yen province, Vietnam. The chemical structures of these compounds were elucidated by NMR spectroscopy as well as compared with data in the literature. The contents of some high yield isolated compounds (2‐4) in the ethanol extract of A. myriophylla were quantified by high‐performance liquid chromatography. These HPLC chromatograms could be used to reveal the presence of isolated compounds as markers of the sample.
Removal efficiency of Fe, Zn and Ni from wastewater of academic campus using hybrid constructed wetlands
Authors: Huong Mai , Van Hoi Bui , Hue Nguyen Thi , Thanh Dinh Thi Mai 
Published: March 2024

AbstractThis study was conducted to investigate the effectiveness of hybrid constructed wetland for removal of Fe, Zin and Ni from wastewater in Vietnam Academy of Science and Technology (VAST), Vietnam. Wastewater samples were collected from the in‐let, out‐let and two filters of the hybrid constructed wetland (HCW) and analyzed for iron (Fe), zinc (Zn), and nickel (Ni) using standard methods. The results indicated that the performance of the HCWs was efficient enough to remove those heavy metals from the wastewater fed to it. HCW‐1 with filter 1 was able to remove average of 41.6 % for Ni, 55.1 % for Fe, and 80.1 % for Zn. Similarly, HCW‐2 with filter 2 removed 51.7 % of Ni, 62.6 % of Fe and 79.3 % of Zn from wastewater. Thus, no significant difference in removing Fe, Zn and Ni from wastewater was observed between HCW‐1 and HCW‐2.
Phenolic compounds from Trigonostemon honbaensis and their cytotoxic activity
Authors: Ban Ninh Khac , Truong Luu Hong , Linh Tran My , Mai Nguyen Chi , Yen Duong Thi Hai , Van Doan Vu , Nhiem Nguyen Xuan , Tai Bui Huu , Van Kiem Phan 
Published: March 2024

AbstractSeven phenolic compounds including (±)‐erythro‐1‐(3′,4′,5′‐trimethoxyphenyl)propane‐1,2‐diol (1), (±)‐threo‐1‐(3′,4′,5′‐trimethoxyphenyl)propane‐1,2‐diol (2), (R)‐3‐3‐(3′,4′‐dimethoxyphenyl)propane‐1,2‐diol (3), (±)‐3‐(3′,4′,5′‐trimethoxyphenyl)propane‐1,2‐diol (4), (+)‐syringaresinol (5), (7S,8R,8′R)‐5,5′‐dimethoxylariciresinol (6), juniperoside (7) were isolated from the leaves of Trigonostemon honbaensis. Their chemical structures were determined using ESI‐MS, 1D NMR, and 2D NMR spectra and in comparison with the reported literature. Compounds1‐ 7 were reported from Trigonostemongenus for the first time. At concentration of 30 μM, compounds 1‐7 exhibited weak cytotoxic activity with cell viability percentages as low as 73.3±0.87 % and 79.5±0.23 % on CAL‐27 and MDA‐MB231 cell lines, respectively.
Chemical composition of pyrolysis oil through thermal decomposition of sugarcane biomass
Authors: Van Nam Huynh , Viet Dinh Quoc , Tam Truong Thanh , Tho Van Dinh Son 
Published: March 2024

AbstractThe process of thermal decomposition of sugarcane biomass consists of 4 stages. On that basis, the research conducted the pyrolysis of sugarcane biomass to collect liquid products at different temperature stages and they were analyzed by GC‐MS method to determine the chemical composition. Chemical composition of pyrolysis oil through thermal decomposition of sugarcane biomass was elucidated in this paper. It can be divided into 6 main groups, including: acids/esters, alcohols, aldehydes/ketones, furanic compounds (furans), phenolic compounds (phenols) and fragments of lignin containing methoxy groups (guaiacols). Acids are obtained mainly at 170 oC, followed by ketones and aldehydes, furans and alcohols, without phenols and guaiacols. At 318 oC, the content of acids decreases, the content of furans, phenols and guaiacols increase, the content of alcohols varies not much and this product segment doesn't contain aldehydes and ketones. The composition of the resulting liquid product varies significantly from 318 to 400 oC with the phenols and guaiacols content account for the majority in liquid products, the rest are furans, alcohols, acids, ketones and aldehydes. The content of phenols and guaiacols in liquid products still accounts for the majority at the temperature stage of 400‐500 oC and 500‐600 oC while the furans content decreases compared to the previous temperature segments. The ring 6 edges compounds of phenols and guaiacols still account for the majority, followed by ring 5 edges compounds of furans, acids, ketones/aldehydes and alcohols in the product mixture of sugarcane pyrolysis at 600 oC.
Sulfanilic acid catalysed one‐pot three‐component Mannich reaction for synthesis of β‐amino ketones
Authors: Kulkarni Pramod 
Published: March 2024

AbstractWe have reported sulfanilic acid as an exceedingly competent catalyst for one‐pot Mannich reaction to give β‐amino carbonyl compounds in good to excellent yield within a short reaction time. The various organic acids have screened; like gallic acid, 4‐hydroxy benzoic acid, 4‐amino benzoic acid, phenylacetic acid, chloroacetic acid, sulfosalicylic acid, sulfanilic acid, chloro benzoic acid, phthalic acid, salicylic acid, cinnamic acid, hippuric acid, 1‐naphthyl acetic acid, o‐amino benzoic acid, p‐TSA, succinic acid, malic acid, and among them sulfanilic acid is a suitable catalyst. The reaction condition was optimized with respect to the solvent, the amount of catalyst as well as the variation of the ketone, aldehyde, and amine substrates. The procedure is mild, effective, ecofriendly, and the use of the minimum amount of catalyst.
Fluorescent properties of some polythiophenes synthesized from 2‐(thiophen‐3‐yl)acetohydrazide and acetophenone
Authors: Linh Nguyen Ngoc , Hung Ha Manh , Oanh Doan Thi Yen , Linh Bui Thi Thuy , Cong Nguyen Tien , Chinh Nguyen Thuy , Hoang Thai , Trung Vu Quoc 
Published: March 2024

AbstractIn this study, new polythiophenes containing hydrazone groups from derivatives of acetophenone were synthesized by chemical oxidative coupling polymerization. Ultraviolet‐visible spectroscopy (UV‐Vis) combined with infrared (IR) analyses proved the supposed structure of novel polythiophenes and proved conformance of the expected synthetic method. Morphology and surface properties of the synthesized polymers were investigated by field‐emission scanning electron microscopy (FE‐SEM). Thermal gravimetry analysis (TGA) has been reported that there was still the presence of small FeCl3 catalyst in polymers and polymers had a stable thermal stability under air atmosphere. The polymers displayed fluorescence emissions at about 590 nm attributed to the π‐conjugated polythiophene. Polymers without doping have a good electrical conductivity (around 4.03×10–7 S/cm at 1 MHz).
Phytochemical investigation of Eurycoma longifolia roots collected in Gia Lai province, Viet Nam
Authors: Ha Vo Khanh , Hanh Truong Thi Minh , Nam Pham Cam , Lien Giang Thi Kim , Chi Mai Thi Phuong , Thao Tran Thi Phuong 
Published: March 2024

AbstractFrom the roots of Eurycoma longifolia, seven compounds including β‐carboline‐2N‐oxide‐1‐propionic acid (1), 9,10‐dimethoxycanthin‐6‐one (2), β‐carboline‐1‐propionic acid (3), infractine (4), eurylene (5), eurycomanone (6), 14,15‐β‐hydroxyklaineanol (7) were isolated. Their structures were determined by means of spectroscopic methods (UV, IR, HR‐ESIMS, 1D and 2D NMR). Compound (1) has been isolated for the first time from nature and compound (4) has been isolated for the first time from Eurycoma longifolia.
Heat treatment for bactericidal particulate silver plating on 316L stainless steel
Authors: Ly Pham Thi , Van Tuan Nguyen , Anh Nguyen Tuan , Thuy Dao Bich , Ha Pham Thi , Cuong Ly Quoc , Quan Vo An 
Published: March 2024

AbstractThe paper presents the impact of heat treatment on the characteristics of the particulate silver plating on 316L stainless steel. Heat treatment was applied in Argon gas media at 500, 600, and 800 oC for 15 minutes. Advanced evaluation methods was applied as scanning electron microscopy (SEM) for surface morphology assessment, tape tests for adhesion determination of silver plating, atomic absorption spectroscopy (AAS) for released concentration of ions in the simulated body fluid (SBF). The antibacterial effect of the silver plating versus Escherichia coli was evaluated through inhibition of bacterial growth after 60 minutes of exposure. The SEM images indicated that there is no change on the grain boundaries after heat treatment. The adhesion of the silver plating increased while increasing temperature from 500 to 800 oC; and the released concentration of silver ion in SBF decreased. The antibacterial effect efficiency of silver plating was from 92 to 99 %. The released concentration of silver ion in SBF after 168 hours was in range of 0.012÷0.18337 μg/mL with average silver plating content was 6.0 mg, much lower in comparison with the allowed threshold of released concentration of silver ion in human body fluid (10 μg/mL).