bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wheat Drought Tolerance: Morpho-Physiological Criteria, Stress Indexes, and Yield Responses in Newly Sand Soils
Authors: Ghanem Hanan Essa , Al-Farouk M. O. 
Published: March 2024

AbstractDrought presents a major risk to wheat growth and productivity under changing climates. During the last few years, various morphological and physiological approaches were used to overcome drought stress-associated problems. Cultivation of tolerant wheat cultivars can serve as a sustainable choice to raise wheat yield under water stress. Herein, field trials were carried out at the experimental farm of Ismailia Agricultural Research Station, Egypt, in two successive growing seasons (2020/2021 and 2021/2022) to investigate the response of four Egyptian bread wheat cultivars (Misr 1, Misr 3, Giza 171, and Sakha 95) to drought stress according to morpho-physiological characteristics, yield, and stress indices. Irrigation treatments and cultivars were assigned to the main and sub-plots, consequently, in a split-plot design with three replicates. The findings revealed that in both the first and second seasons, drought drastically revoked growth vigor of shoot, growth vigor of flag leaf, relative water content (%), membrane stability index (%), photosynthetic pigments, heading (days), maturity (days), as well as yield and yield attributes: spike length, number of spikes/m2, spike weight, grain number/spike, 100-kernel weight, grain yield/m2, straw yield/m2, biological yield/m2, and harvest index of all four wheat cultivars. Conversely, drought caused a marked increase in saturation water deficit (%), carotenoids content, and NKP uptake of all four wheat cultivars in both study seasons. The current study found that all four of the wheat cultivars were drought-tolerant plants. These cultivars exhibited similar drought-tolerant behaviors, which included decreased loss in relative water content, membrane stability, and photosynthetic pigment levels, consequently reducing wheat grain yield loss under water stress. Additionally, the drought tolerance indices of Sakha 95 > Giza 171 > Misr 1 > Misr 3 were revealed by the stress sensitivity index (SSI), mean productivity (MP), stress tolerance index (STI), and yield stability index (YSI). In conclusion, Misr 3 was the least tolerant wheat cultivar and Sakha 95 was the most tolerant. These results can be applied to breeding programs by plant breeders.
Automated shape and thickness optimization for non-matching isogeometric shells using free-form deformation
Authors: Zhao Han , Kamensky David , Hwang John T. , Chen Jiun-Shyan 
Published: March 2024

AbstractIsogeometric analysis (IGA) has emerged as a promising approach in the field of structural optimization, benefiting from the seamless integration between the computer-aided design (CAD) geometry and the analysis model by employing non-uniform rational B-splines (NURBS) as basis functions. However, structural optimization for real-world CAD geometries consisting of multiple non-matching NURBS patches remains a challenging task. In this work, we propose a unified formulation for shape and thickness optimization of separately parametrized shell structures by adopting the free-form deformation (FFD) technique, so that continuity with respect to design variables is preserved at patch intersections during optimization. Shell patches are modeled with isogeometric Kirchhoff–Love theory and coupled using a penalty-based method in the analysis. We use Lagrange extraction to link the control points associated with the B-spline FFD block and shell patches, and we perform IGA using the same extraction matrices by taking advantage of existing finite element assembly procedures in the FEniCS partial differential equation (PDE) solution library. Moreover, we enable automated analytical derivative computation by leveraging advanced code generation in FEniCS, thereby facilitating efficient gradient-based optimization algorithms. The framework is validated using a collection of benchmark problems, demonstrating its applications to shape and thickness optimization of aircraft wings with complex shell layouts.
A Tutorial Review of the Solar Power Curve: Regressions, Model Chains, and Their Hybridization and Probabilistic Extensions
Authors: Yang Dazhi , Xia Xiang’ao , Mayer Martin János 
Published: March 2024

AbstractOwing to the persisting hype in pushing toward global carbon neutrality, the study scope of atmospheric science is rapidly expanding. Among numerous trending topics, energy meteorology has been attracting the most attention hitherto. One essential skill of solar energy meteorologists is solar power curve modeling, which seeks to map irradiance and auxiliary weather variables to solar power, by statistical and/or physical means. In this regard, this tutorial review aims to deliver a complete overview of those fundamental scientific and engineering principles pertaining to the solar power curve. Solar power curves can be modeled in two primary ways, one of regression and the other of model chain. Both classes of modeling approaches, alongside their hybridization and probabilistic extensions, which allow accuracy improvement and uncertainty quantification, are scrutinized and contrasted thoroughly in this review.
Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit
Authors: Kant Kamal , Rigó Gábor , Faragó Dóra , Benyó Dániel , Tengölics Roland , Szabados László , Zsigmond Laura 
Published: March 2024

Abstract
Main Conclusion
The Arabidopsis Pentatricopeptide repeat 40 (PPR40) insertion mutants have increased tolerance to water deficit compared to wild-type plants. Tolerance is likely the consequence of ABA hypersensitivity of the mutants.

Abstract
Plant growth and development depend on multiple environmental factors whose alterations can disrupt plant homeostasis and trigger complex molecular and physiological responses. Water deficit is one of the factors which can seriously restrict plant growth and viability. Mitochondria play an important role in cellular metabolism, energy production, and redox homeostasis. During drought and salinity stress, mitochondrial dysfunction can lead to ROS overproduction and oxidative stress, affecting plant growth and survival. Alternative oxidases (AOXs) and stabilization of mitochondrial electron transport chain help mitigate ROS damage. The mitochondrial Pentatricopeptide repeat 40 (PPR40) protein was implicated in stress regulation as ppr40 mutants were found to be hypersensitive to ABA and high salinity during germination. This study investigated the tolerance of the knockout ppr40-1 and knockdown ppr40-2 mutants to water deprivation. Our results show that these mutants display an enhanced tolerance to water deficit. The mutants had higher relative water content, reduced level of oxidative damage, and better photosynthetic parameters in water-limited conditions compared to wild-type plants. ppr40 mutants had considerable differences in metabolic profiles and expression of a number of stress-related genes, suggesting important metabolic reprogramming. Tolerance to water deficit was also manifested in higher survival rates and alleviated growth reduction when watering was suspended. Enhanced sensitivity to ABA and fast stomata closure was suggested to lead to improved capacity for water conservation in such environment. Overall, this study highlights the importance of mitochondrial functions and in particular PPR40 in plant responses to abiotic stress, particularly drought.
Influence of gastric residual assessment in preterm neonates on time to achieve enteral feeding (the GRASS trial)—Multi-centre, assessor-blinded randomised clinical trial
Authors: Branagan A , Murphy C , O’Sullivan A , Bodnarova I , Feyereislova S , Berka I , Miletin J , Stranak Z 
Published: March 2024

Abstract
Purpose
Gastric residual measurement is routinely performed in premature infants prior to feeding despite a lack of evidence of benefit. We aimed to evaluate if the exclusion of routine gastric residual measurement and evaluation has an impact on the time taken to achieve full enteral feeding in preterm neonates.

Methods
International multi-centre randomised controlled trial. Clinically stable, appropriate for gestational age infants between 26+0 and 30+6 weeks of gestation and less than 1.5 kg birth weight were eligible. Infants were randomised to the intervention arm (no monitoring of gastric aspirates) or control arm (routine care). Primary outcome was the achievement of enteral feeds of 100 ml/kg/day by day 5 of life.

Results
Ninety-five infants were recruited with 88 included in an intention-to-treat analysis, 45 in the intervention arm and 43 in the control arm. There was no imbalance in baseline characteristics. Thirty-three (73.3%) infants in the intervention group and 32 infants (74.4%) in the control group reached full feeds by day 5 of life (p = 0.91) with no difference in median time to full feeds. There were no statistically significant differences in survival or the major morbidities of prematurity.
Conclusion: There was no difference in time to attainment of enteral feeds of 100 ml/kg/day in premature infants when gastric residuals were not monitored. In the absence of a clinical benefit to routine monitoring, it may be appropriate to discontinue this practice and only monitor residuals when clinical concern of feeding intolerance or gastrointestinal pathology arises in this group of patients.
Trial registration: NCT03111329—https://clinicaltrials.gov/. Registered 06/04/2017.

What is Known:• Previous randomized trials have shown little benefit to the performance of routine assessment of gastric residuals in preterm infants. Despite this, they continue to be performed due to concerns from observational data regarding development of NEC. Meta-analysis to date has failed to answer the question regarding NEC.

What is New:• In very low birth weight infants who are fed using modern feeding practice of faster feed advancement, to minimize use of central access and parenteral nutrition, exclusion of routine checks of gastric residuals did not increase the proportion of infants reaching full enteral feeds by day 5. No harm was seen when residual checks were not performed.• In the absence of a clinical benefit to the routine performance of gastric residuals in very low birth weight infants, it may be appropriate to discontinue their use and instead check residuals when clinical concern of pathology arises.
A rare Ewing-like small round cell tumor in prostate: a case report and literature review
Authors: Wang Zhen , Ye Jian , Hu Junjie , Zhang Nan , Yuan Yichu 
Published: March 2024

Abstract
Background
Small round cell tumor (SRCT) is a group of malignancy with similar optical microscopic morphology. Despite its low incidence, SRCT has a high malignant degree and poor prognosis. Besides, atypical clinical symptoms make it difficult in preoperative diagnosis.

Case report
A 67-year-old man was presented to the outpatient service with dysuria and weak urine stream lasting for 3 months. After oral treatment with tamsulosin and finasteride for 2 months, the symptoms worsen. Transurethral prostate holmium laser enucleation was operated and postoperative pathology result revealed small blue round cell malignant tumor. Further immunohistochemistry and fluorescence in situ hybridization examination indicated Ewing-like SRCT. So a Da Vinci Robotic prostatectomy was performed further and whole-genome sequencing was conducted. Several gene mutations including RAF1, ARID1A, SMARCA4, and BCL2L11 were found but no FDA-approved drug could treat specifically. Then the patient received Ewing-type therapeutic regimens treatment and has been followed up to date (over 24 months).

Conclusion
Because of its non-elevated serum PSA level, prostate SRCT is often ignored as a possibility of malignant tumor and regarded as benign prostatic hyperplasia (BPH). The possibility of prostate SRCT need to be considered if dysuria symptoms could not alleviate significantly after a period of oral treatment.
Integrated machine learning-driven disulfidptosis profiling: CYFIP1 and EMILIN1 as therapeutic nodes in neuroblastoma
Authors: Mengzhen Zhang , Xinwei Hou , Zeheng Tan , Nan Li , Yang Yang , Huirong Yang , Kaisi Fan , Xiaoting Ding , Liucheng Yang , Kai Wu 
Published: March 2024

Abstract
Background
Neuroblastoma (NB), a prevalent pediatric solid tumor, presents formidable challenges due to its high malignancy and intricate pathogenesis. The role of disulfidptosis, a novel form of programmed cell death, remains poorly understood in the context of NB.

Methods
Gaussian mixture model (GMM)-identified disulfidptosis-related molecular subtypes in NB, differential gene analysis, survival analysis, and gene set variation analysis were conducted subsequently. Weighted gene co-expression network analysis (WGCNA) selected modular genes most relevant to the disulfidptosis core pathways. Integration of machine learning approaches revealed the combination of the Least absolute shrinkage and selection operator (LASSO) and Random Survival Forest (RSF) provided optimal dimensionality reduction of the modular genes. The resulting model was validated, and a nomogram assessed disulfidptosis characteristics in NB. Core genes were filtered and subjected to tumor phenotype and disulfidptosis-related experiments.

Results
GMM clustering revealed three distinct subtypes with diverse prognoses, showing significant variations in glucose metabolism, cytoskeletal structure, and tumor-related pathways. WGCNA highlighted the red module of genes highly correlated with disulfide isomerase activity, cytoskeleton formation, and glucose metabolism. The LASSO and RSF combination yielded the most accurate and stable prognostic model, with a significantly worse prognosis for high-scoring patients. Cytological experiments targeting core genes (CYFIP1, EMILIN1) revealed decreased cell proliferation, migration, invasion abilities, and evident cytoskeletal deformation upon core gene knockdown.

Conclusions
This study showcases the utility of disulfidptosis-related gene scores for predicting prognosis and molecular subtypes of NB. The identified core genes, CYFIP1 and EMILIN1, hold promise as potential therapeutic targets and diagnostic markers for NB.
Dynamic relationship among extracellular matrix and body wall cells in Hirudo verbana morphogenesis
Authors: Pulze Laura , Baranzini Nicolò , Acquati Francesco , Marcolli Gaia , Grimaldi Annalisa 
Published: March 2024

AbstractA great bulk of recent experimental evidence suggests the key role of the complex crosstalk between the extracellular matrix (ECM) and the cellular component of tissues during morphogenesis and embryogenesis. In particular, remodeling of the ECM and of its physical interactions pattern with surrounding cells represent two crucial processes that might be involved in muscle development. However, little information is available on this topic, especially on invertebrate species. To obtain new insights on how tuning the ECM microenvironment might drive cellular fate during embryonic development, we used the invertebrate medicinal leech Hirudo verbana as a valuable experimental model, due to its simple anatomy and the recapitulation of many aspects of the basic biological processes of vertebrates. Our previous studies on leech post-embryonic development have already shown the pivotal role of ECM changes during the growth of the body wall and the role of Yes-associated protein 1 (YAP1) in mechanotransduction. Here, we suggest that the interactions between stromal cell telocytes and ECM might be crucial in driving the organization of muscle layers during embryogenesis. Furthermore, we propose a possible role of the pleiotropic enzyme HvRNASET2 as a possible modulator of collagen deposition and ECM remodeling not only during regenerative processes (as previously demonstrated) but also in embryogenesis.
Comparison of estimated GFR using cystatin C versus creatinine in pediatric kidney transplant recipients
Authors: Pizzo Helen , Nguyen John , Schwartz George J. , Wesseling-Perry Katherine , Ettenger Robert , Chambers Eileen Tsai , Weng Patricia 
Published: March 2024

Abstract
Background
An accurate, rapid estimate of glomerular filtration rate (GFR) in kidney transplant patients affords early detection of transplant deterioration and timely intervention. This study compared the performance of serum creatinine (Cr) and cystatin C (CysC)-based GFR equations to measured GFR (mGFR) using iohexol among pediatric kidney transplant recipients.

Methods
CysC, Cr, and mGFR were obtained from 45 kidney transplant patients, 1–18 years old. Cr- and CysC-estimated GFR (eGFR) was compared against mGFR using the Cr-based (Bedside Schwartz, U25-Cr), CysC-based (Gentian CysC, CAPA, U25-CysC), and Cr-CysC combination (CKiD Cr-CysC, U25 Cr-CysC) equations in terms of bias, precision, and accuracy. Bland–Altman plots assessed the agreement between eGFR and mGFR. Secondary analyses evaluated the formulas in patients with biopsy-proven histological changes, and K/DOQI CKD staging.

Results
Bias was small with Gentian CysC (0.1 ml/min/1.73 m2); 88.9% and 37.8% of U25-CysC estimations were within 30% and 10% of mGFR, respectively. In subjects with histological changes on biopsy, Gentian CysC had a small bias and U25-CysC were more accurate—both with 83.3% of and 41.7% of estimates within 30% and 10% mGFR, respectively. Precision was better with U25-CysC, CKiD Cr-CysC, and U25 Cr-CysC. Bland–Altman plots showed the Bedside Schwartz, Gentian CysC, CAPA, and U25-CysC tend to overestimate GFR when > 100 ml/min/1.72 m2. CAPA misclassified CKD stage the least (whole cohort 24.4%, histological changes on biopsy 33.3%).

Conclusions
In this small cohort, CysC-based equations with or without Cr may have better bias, precision, and accuracy in predicting GFR.

Graphical abstract
Radiofrequenzdenervation der Wirbelsäule und des Iliosakralgelenks
Authors: Klessinger Stephan , Casser Hans-Raimund , Gillner Sebastian , Koepp Holger , Kopf Andreas , Legat Martin , Meiler Katharina , Norda Heike , Schneider Markus , Scholz Matti , Slotty Phillipp J. , Tronnier Volker , Vazan Martin , Wiechert Karsten 
Published: March 2024

Zusammenfassung
Hintergrund
In diesem Artikel werden die Ergebnisse der S3-Leitlinie „Radiofrequenzdenervation der Facettengelenke und des ISG“ zusammengefasst. Die vorhandene Evidenz zur Indikation, zu Testblockaden und zu technischen Parametern wird dargelegt.

Ziel
Es soll sowohl einer Über- als auch eine Unterversorgung entgegengewirkt werden, was auch sozioökonomische Bedeutung hat.

Material und Methode
Es erfolgte eine systematische Auswertung der Literatur nach den Grading of Recommendations, Assessment, Development, and Evaluation (GRADE). Eine multidisziplinäre Leitliniengruppe hat Empfehlungen und Statements ausgearbeitet.

Ergebnisse
Für 20 Schlüsselfragen wurden Statements und Empfehlungen formuliert. Es gab 87,5 % Konsens für eine Empfehlung und 100 % Konsens für alle weiteren Empfehlungen und Statements. Die Leitlinie wurde von allen beteiligten Fachgesellschaften konsentiert. Die spezifischen Fragen beinhalten den Wert von Anamnese, Untersuchung und Bildgebung, die Notwendigkeit einer konservativen Therapie vor einer Intervention, die Bedeutung von Testblockaden („medial branch block“ und „lateral branch block“), die Wahl der Bildgebung für eine Denervation, die Wahl der Trajektorie, die Möglichkeit, die Größe der Läsion zu beeinflussen, Stimulation, die Möglichkeit einer Wiederholung, Sedierung und Entscheidungshilfen zu Patienten mit Antikoagulanzien, Metallimplantaten und Schrittmachern und Hinweise zur Vermeidung von Komplikationen.

Schlussfolgerungen
Ausgewählte Patienten können von einer gut durchgeführten Radiofrequenzdenervation profitieren. Die Empfehlungen der Leitlinie basieren auf sehr niedriger bis moderater Qualität der Evidenz.
High-resolution projections of outdoor thermal stress in the twenty-first century: a Tasmanian case study
Authors: Weeding Ben , Love Peter , Beyer Kathleen , Lucieer Arko , Remenyi Tom 
Published: March 2024

AbstractTo adapt to Earth’s rapidly changing climate, detailed modelling of thermal stress is needed. Dangerous stress levels are becoming more frequent, longer, and more severe. While traditional measurements of thermal stress have focused on air temperature and humidity, modern measures including radiation and wind speed are becoming widespread. However, projecting such indices has presented a challenging problem, due to the need for appropriate bias correction of multiple variables that vary on hourly timescales. In this paper, we aim to provide a detailed understanding of changing thermal stress patterns incorporating modern measurements, bias correction techniques, and hourly projections to assess the impact of climate change on thermal stress at human scales. To achieve these aims, we conduct a case study of projected thermal stress in central Hobart, Australia for 2040–2059, compared to the historical period 1990–2005. We present the first hourly metre-scale projections of thermal stress driven by multivariate bias-corrected data. We bias correct four variables from six dynamically downscaled General Circulation Models. These outputs drive the Solar and LongWave Environmental Irradiance Geometry model at metre scale, calculating mean radiant temperature and the Universal Thermal Climate Index. We demonstrate that multivariate bias correction can correct means on multiple time scales while accurately preserving mean seasonal trends. Changes in mean air temperature and UTCI by hour of the day and month of the year reveal diurnal and annual patterns in both temporal trends and model agreement. We present plots of future median stress values in the context of historical percentiles, revealing trends and patterns not evident in mean data. Our modelling illustrates a future Hobart that experiences higher and more consistent numbers of hours of heat stress arriving earlier in the year and extending further throughout the day.
Characterization of percutaneous absorption of calcium, magnesium, and potentially toxic elements in two tailored sulfurous therapeutic peloids: a comprehensive in vitro pilot study
Authors: Bastos Carla Marina , Rocha Fernando , Patinha Carla , Marinho-Reis Paula 
Published: March 2024

AbstractPelotherapy treatments in thermal spas, which utilize peloids composed of clay minerals mixed with saltwater or mineral-medicinal water, can have various effects on spa users, ranging from therapeutic to potential adverse reactions. Despite the widespread use of peloids, comprehensive information on the penetration and permeation of essential and potentially toxic elements into deeper layers of the skin during pelotherapy is limited. Understanding the concentrations of these elements is crucial for evaluating therapeutic benefits and ensuring safety. This study investigates the in vitro availability and absorption of calcium, magnesium, and potentially toxic elements in two peloids, considering their formulation matrix. To replicate the pelotherapy methodology, an in vitro permeation experiment was conducted using a vertical diffusion chamber (Franz cells) and a biological system with human skin membranes from five Caucasian women, age range between 25 and 51 years. The experiment involved heating the peloids to 45℃. The results emphasize the possible transport properties of chemical elements in peloids, providing valuable information related to potential therapeutic efficacy and safety considerations. Despite no apparent differences between peloids’ chemical composition, the method identified permeation variations among chemical elements. The methodology employed in this study adheres to the guidelines outlined by OECD for analyzing skin absorption through an in vitro approach. Furthermore, it aligns with the associated OECD guidance document for conducting skin absorption studies. The replicability of this methodology not only facilitates the analysis of peloids pre-formulation but also provides a robust means to evaluate the effectiveness of therapeutic elements during topical administration, particularly those with potential toxicity concerns.