bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Genetic Basis of Gelatinization Temperature of Rice
Authors: Tan Yifang , Xing Yongzhong , Zhang Qifa , Sun Mei , Corke Harold 
Published: June 2007

ABSTRACTGelatinization temperature of rice starch plays an important role in grain quality. We mapped eight loci responsible for the gelatinization parameters of rice: onset temperature (To), peak temperature (Tp), conclusion temperature (Tc) and enthalpy (ΔH) using a population of 242 recombinant inbred lines (RIL). Eight quantitative trait loci (QTL) were mapped onto the chromosome regions where starch‐synthesis related genes are located. The Wx gene (granule‐bound starch synthase [GBSS]), and the region of chromosome 11 played major roles in gelatinization trait determination; and other loci were starch‐branching enzyme (SBE) genes that modify the two major loci by epistasis. High‐level interactions were also detected indicating a trans mode of action of the genes. The genetic basis for these parameters was consistent with the relationship between the thermal properties and the structure of the starch granule. This information can be helpful for the improvement of starch‐producing crops, including the improvement of rice grain quality, both for food and industrial uses.
Influence of Amylose Content on Properties of Wheat Starch and Breadmaking Quality of Starch and Gluten Blends
Authors: Lee Mee‐Ryung , Swanson Barry G. , Baik Byung‐Kee 
Published: June 2007

ABSTRACTThe effects of amylose content on thermal properties of starches, dough rheology, and bread staling were investigated using starch of waxy and regular wheat genotypes. As the amylose content of starch blends decreased from 24 to 0%, the gelatinization enthalpy increased from 10.5 to 15.3 J/g and retrogradation enthalpy after 96 hr of storage at 4°C decreased from 2.2 to 0 J/g. Mixograph water absorption of starch and gluten blends increased as the amylose content decreased. Generally, lower rheofermentometer dough height, higher gas production, and a lower gas retention coefficient were observed in starch and gluten blends with 12 or 18% amylose content compared with the regular starch and gluten blend. Bread baked from starch and gluten blends exhibited a more porous crumb structure with increased loaf volume as amylose content in the starch decreased. Bread from starch and gluten blends with amylose content of 19.2–21.6% exhibited similar crumb structure to that of bread with regular wheat starch which contained 24% amylose. Crumb moisture content was similar at 5 hr after baking but higher in bread with waxy starch than in bread without waxy starch after seven days of storage at 4°C. Bread with 10% waxy wheat starch exhibited lower crumb hardness values compared with bread without waxy wheat starch. Higher retrogradation enthalpy values were observed in breads containing waxy wheat starch (4.56 J/g at 18% amylose and 5.43 J/g at 12% amylose) compared with breads containing regular wheat starch (3.82 J/g at 24% amylose).
Separation and Quantification of HMW Glutenin Subunits by Capillary Electrophoresis
Authors: Zhu J. , Khan K. 
Published: June 2007

ABSTRACTThe use of capillary electrophoresis in SDS (SDS‐CE) for separation and quantification of HMW glutenin subunits (HMW‐GS) was investigated. HMW‐GS were precipitated with 40% acetone from 50% 1‐propanol extract of flour under reducing conditions after removal of monomeric proteins with 50% 1‐propanol. Poly (ethylene oxide) was used in the running buffer (3% w/v) for SDS‐CE. The results indicated that HMW‐GS could be well separated by SDS‐CE, including subunits 7+8, 7+9, 2+12, 5+10, and 17+18. However, HMW‐GS showed delayed migration times compared with molecular weight protein standards. Some HMW‐GS were reversed in their mobilities in SDS‐CE compared with their mobility and molecular weights by SDS‐PAGE. Therefore, the SDS‐CE was unsuitable for MW determination of HMW‐GS. A linear response was obtained from SDS‐CE of a plot of the concentration of HMW‐GS of the 40% acetone precipitate versus corrected areas for absorbance at 214 nm. Quantification of HMW‐GS for the two biotypes (subunits 5+10 vs. 2+12) of an Australian wheat cultivar Warigal confirmed the differences between the two biotypes in their quantity of HMW‐GS. Therefore, the technique could be used to quantify HMW‐GS in conjunction with SDS‐PAGE.
Two‐Dimensional Vibration Spectroscopy of Rice Quality and Cooking
Authors: Barton F. E. , Himmelsbach D. S. , McClung A. M. , Champagne E. L. 
Published: June 2007

ABSTRACTRice samples were taken from a study of rice milling properties that affect quality. The spectra of milled and cooked samples were taken in the near‐infrared, mid‐infrared, and Raman region. These spectra, two regions at a time, were regressed by a two‐dimensional technique to develop contour maps that indicated the correlation of two spectral regions. These relationships demonstrate that it is possible to recognize the hydration effects caused by gelatinization (cooked samples vs. milled rice). Three water (O‐H stretch) spectral bands (960, 1445, 1,930 nm) in the near‐infrared (NIR) show marked differences between milled and cooked rice. The difference spectra indicated that there were additional phenomena occurring besides the addition of water. These differences are apparent in both C‐O‐H and N‐H bands, which indicate that water is interacting with both starch and protein. The two‐dimensional technique developed in this laboratory was used to get a better interpretation of what occurs during cooking. The Raman spectrum, which is relatively insensitive to water (O‐H stretch), revealed only changes in protein that could be associated with denaturization.
Plant Sterols in Cereals and Cereal Products
Authors: Piironen V. , Toivo J. , Lampi A.‐M. 
Published: June 2007

ABSTRACTThe total plant sterol contents (free sterols and covalently bound structures) of the main cereals cultivated in Finland were determined. Furthermore, sterol contents were determined for different flour and bran fractions in the milling process of wheat and rye, as well as plant sterol contents in various milling and retail bakery products. The sample preparation procedure included acid and alkaline hydrolysis to liberate sterols from their glycosides and esters, respectively. Free sterols were extracted and, after recovery using solid‐phase extraction, derivatized to trimethylsilyl ethers for gas chromatography (GC) analysis. We used GC with a mass spectrometer (MS) for identification. When two cultivars of rye, wheat, barley, and oats grown in the same year were compared, the highest plant sterol content was observed in rye (mean content 95.5 mg/100 g, wb), whereas the total sterol contents (mg/100 g, wb) of wheat, barley, and oats were 69.0, 76.1, and 44.7, respectively. In addition, the 10 rye cultivars and breeding lines compared had total sterol contents of 70.7–85.6 mg/100 g. In the milling process of rye and wheat, the plant sterols fractionated according to the ash content of the corresponding milling product. In all cereal grain and milling product samples, sitosterol was the main sterol. The level of stanols differed in the different milling process samples; it was lower in the most refined rye and wheat flours (≈15%) than in the bran fractions (≈30% in the bran with 4% ash content). Rye bread with whole meal rye flour as the main or only ingredient was a good source of sterols. Sterol content was higher than that of wheat bread, whereas plant sterol content of other bakery products was affected by the type and amount of fat used in baking.
Tetraploid Wheat—A Resource for Genetic Improvement of Durum Wheat Quality
Authors: Sissons M. J. , Hare R. A. 
Published: June 2007

ABSTRACTThe tetraploid relatives (subspecies) of commercial durum wheat (Triticum turgidum L. subsp. turgidum conv. durum (Desf.) MacKey) offer a source of economically useful genes for the genetic improvement of durum cultivars. Tetraploid wheat subspecies show a wide diversity in grain protein composition and content, which are major factors determining the pasta‐making quality of durum cultivars. In this study, the specific focus was the identification of accessions expressing one or more superior pasta‐making traits. In all, 33 accessions were surveyed representing five different subspecies; var. durum (13 accessions), polonicum (7 accessions), persicum (3 accessions), turanicum (6 accessions), and turgidum (4 accessions). These accessions and the durum cultivars Wollaroi and Kamilaroi (in both years) and Yallaroi (in 1998 only) were grown at Tamworth, Australia in 1997 and 1998. Grain, semolina, and spaghetti cooking quality were evaluated using a range of tests. Several accessions were identified with larger grain size and protein content and higher semolina extraction. Although many of the accessions were weaker in dough strength, a few were equal to the commercial cultivars and produced pasta of comparable quality. The main disadvantage with these accessions was the low yellow color. These quality defects can be corrected by conventional breeding.
Swelling Index of Glutenin Test. II. Application in Prediction of Dough Properties and End‐Use Quality
Authors: Wang C. , Kovacs M. I. P. 
Published: June 2007

ABSTRACTSmall‐scale tests, including SDS and Zeleny sedimentation, gel protein, insoluble glutenin content, and a newly developed method, the swelling index of glutenin (SIG), were compared with dough and gluten rheological parameters and end‐use quality parameters for 20 wheat cultivars or breeders lines. The SIG test is equal to or slightly better than the other small‐scale tests in prediction of dough strength. Quality parameters were divided into two groups according to associations with insoluble glutenin content and glutenin quality. The glutenin quality is defined as the glutenin swelling properties with short swelling time (≤5 min) that are contributed by soluble and insoluble glutenin content and their swelling properties. Parameters in the first group were mainly dependent on insoluble glutenin content and appeared to reflect gluten strength. Parameters in the second group were dependent not only on glutenin content, but also on glutenin quality. Small‐scale tests are best to predict quality parameters within the same group, but not those in the other group. The glutenin swelling curve, obtained with different swelling times, was correlated with mixograph or farinograph data. Dough development time in farinograph and mixing time in mixograph were strongly related to the swelling time of peak SIG value in the swelling curve (r = 0.92, r = 0.86, respectively, P < 0.001). Farinograph stability was significantly related to the time of swollen stage in swelling curves (r = 0.62, P < 0.01). Similar to mixograph or farinograph data, the glutenin swelling curves can be used to differentiate some strong cultivars that can not be differentiated by sedimentation, gel protein, and insoluble glutenin values.
Swelling Index of Glutenin Test for Prediction of Durum Wheat Quality
Authors: Wang C. , Kovacs M. I. P. 
Published: June 2007

ABSTRACTThe swelling index of glutenin (SIG) was assessed for suitability as a screening procedure for durum wheat gluten strength. Five sets of samples with a wide range of gluten strength values were collected according to gluten strength parameters and characterized for SIG. Statistical analysis revealed that the SIG and SDS sedimentation tests were both able to satisfactorily account for variations in gluten strength in all sets of samples. However, for most samples, the SIG test was more reliable than the SDS sedimentation test for predicting gluten strength. Furthermore, the SIG test can differentiate samples with glutenin swelling properties that could not be accounted for by intercultivar variation of SDS sedimentation volumes. The percentage of insoluble glutenin in protein is usually a better predictor for gluten strength than the percentage of insoluble glutenin in flour. Similarly, when the SIG values were divided by the protein content of the samples, the resulting proportions were better predictors of gluten strength than the absolute values. Analysis of protein fractions revealed that the insoluble glutenin was the protein fraction most responsible for the SIG value and gluten strength. Extensibility of dough was significantly related to the soluble glutenin content, while the alveograph G index was related to monomeric protein content. The results suggest that screening based on the SIG test would be valuable for comparing durum wheat lines and cultivars for gluten strength and pasta‐making quality.
Effect of Partial Waxy Wheat on Processing and Quality of Wheat Flour Tortillas
Authors: Waniska Ralph D. , Graybosch Robert A. , Adams Janet L. 
Published: June 2007

ABSTRACTThe processing and quality of wheat flour tortillas prepared with partial waxy and normal flour were evaluated. Control procedures and formula were utilized with water absorption varied to obtain machineable doughs. Amylose content was lower in most partial waxy compared with normal wheats. The type of wheat starch did not affect most dough properties or tortilla diameter. Tortilla height and opacity were adversely affected by the decreased amount of amylose in partial waxy wheats. Sufficient leavening reactions occurred early in baking (after 10 sec) to yield an opaque disk, but some baked tortillas lost opacity and become partially transparent after baking. Starch gelatinizes, disperses, and retrogrades concurrently with the leavening reaction during the short (<30 sec) baking time. Amylose functionality during baking and cooling appears to be involved in the retention of air bubbles in tortillas.
Characterization of Italian Durum Wheat Semolina by Means of Chemical Analytical and Spectroscopic Determinations
Authors: Brescia Maria Antonietta , Di Martino Giuliano , Fares Clara , Di Fonzo Natale , Platani Cristiano , Ghelli Stefano , Reniero Fabiano , Sacco Antonio 
Published: June 2007

ABSTRACTProton high‐resolution magic angle spinning nuclear magnetic resonance (1H HR‐MAS NMR) has been applied for the analysis of two cultivars of durum wheat produced in different Italian geographical areas. Furthermore, on the same samples, isotopic ratios were measured by mass spectrometry (IRMS). The application of chemometrics to these results permitted the discrimination of semolina by cultivar and geographical origin. A similar approach has been applied to the results obtained from chemical analyses. The comparison showed that NMR spectroscopy can provide a faster method for the detection of differences among the durum wheat semolina according to geographical and varietal origin. Furthermore, IRMS data are suitable to provide information about the geographical origin of samples. This present investigation is part of an extensive research project to find a scientific method capable of classifying wheat foods for the assignment of a “denomination of origin” trademark.
Influence of Fat on Expansion of Glassy Amylopectin Extrudates by Microwave Heating
Authors: Ernoult V. , Moraru C. I. , Kokini J. L. 
Published: June 2007

ABSTRACTThe effect of solid fat (melting point 70°C) and liquid fat (melting point ‐20°C) on the microwave expansion of extruded glassy amylopectin pellets was investigated. Glassy, unexpanded amylopectin extrudates containing ≤10% fat were equilibrated at different water activity levels (aw) and then expanded by microwave heating. Expanded products were characterized by analyzing degree of expansion, structure, and texture. For the aw range studied, expansion increased with increasing aw of the pellets until a maximum at aw = 0.605 and then decreased. Completely dehydrated samples did not expand at all. This demonstrated that moisture was the driving force in the microwave expansion of the pellets. The addition of solid fat contributed greatly to expansion. The highest degree of expansion was obtained for pellets containing 6% solid fat at all aw levels. Due to the effect on expansion, the addition of solid fat also affected the microstructure and texture of the expanded products. Concentrations of solid fat >2% resulted in expanded products with a large number of cells, uniform in size, with thin cell walls. Liquid fat addition had a negative effect on microwave expansion. The expanded products obtained from pellets that contained liquid fat had a lower expansion volume and a coarser structure.
Effect of Small and Large Wheat Starch Granules on Thermomechanical Behavior of Starch
Authors: Chiotelli Eleni , Le Meste Martine 
Published: June 2007

ABSTRACTThe physicochemical properties of small‐ and large‐granule wheat starches were investigated to reveal whether gelatinization properties and rheological behavior differ between size classes of wheat starch. All samples contained 60% water (w/w, wb). The starch granule size and shape were examined by scanning electron microscopy in the separated A‐ and B‐type granule populations and in the whole wheat starch granule population. Differential scanning calorimetry (DSC) and electron spin resonance (ESR) analyses were performed in parallel with rheological measurements using dynamic mechanical thermal analysis (DMTA) to relate the viscoelastic changes to modifications in dynamic properties of aqueous solutions and structural disorganization of starch. The small (B‐type) granules had slightly higher gelatinization temperature and lower gelatinization enthalpy than did the large (A‐type) granules. Also, B‐type granules had higher enthalpy for the amylose‐lipid complex transition. Moreover, our results suggested that small granules have higher affinity for water at room temperature. It seems that there is a less ordered arrangement of the polysaccharide chains in the smaller granules when compared with the larger ones. These differences in functional properties of small and large granules suggested that the granule size distribution is an important parameter in the baking process.
Effect of Storage Time on In Vitro Digestibility and Resistant Starch Content of Nixtamal, Masa, and Tortilla
Authors: Rendon‐Villalobos Rodolfo , Bello‐Pérez Luis Arturo , Osorio‐Díaz Perla , Tovar Juscelino , Paredes‐López Octavio 
Published: June 2007

ABSTRACTNixtamal, masa, and tortilla samples were stored for 24–96 hr and their chemical composition, retrogradation, and in vitro starch digestibility features were evaluated. Ash and fat contents in the three products were smaller than in the original corn sample, but protein levels were higher, all in accordance with previous studies. In general, a minor decrease in available starch (AS) content was observed with storage time. Masa showed the greatest AS values, followed by tortilla and nixtamal. Tortilla presented slightly higher retrograded resistant starch (RS3) values (1.1–1.8%, dmb) than masa (0.7–0.9%) and nixtamal (0.7–0.8%) and only minor increases were observed after 24 hr of storage, suggesting that retrogradation phenomenon in these samples takes place very rapidly and is more pronounced in the final product (tortilla). The development of RS3 explains the observed decrease in AS. Higher total resistant starch values were found in all samples at a range of 2.1–2.6% for nixtamal and masa, and a range of 3.1–3.9% in tortilla. This indicates that, apart from retrograded resistant starch, some ungelatinized fractions appear to contibute to the indigestible content of these products. The α‐amylolysis rate of the three materials decreased with storage. Tortilla showed the greatest hydrolysis indices. Differential scanning calorimetry (DSC) analysis showed that the nixtamal, masa, and tortilla did not show differences in amylopectin crystal melting temperature with storage time, but tortilla exhibited higher enthalpy values after 72 hr of storage, in accordance with the greater total RS contents recorded after prolonged storage.
Structural Characteristics of Water‐Extractable Nonstarch Polysaccharides from Barley Malt
Authors: Cyran M. , Izydorczyk M. S. , MacGregor A. W. 
Published: June 2007

ABSTRACTWater‐extractable (WE) material was isolated from a Canadian barley malt (cv. Harrington). The purified WE material contained mainly arabinoxylans, β‐glucans, proteins, and small amounts of arabinogalactans and mannose‐containing polymers. WE material was treated with specific enzymes to obtain two fractions: one enriched in arabinoxylan (AX) and another enriched in β‐glucan (BG). The AX fraction was further fractionated by stepwise precipitation in (NH4)2SO4 into five arabinoxylan subfractions. 1H‐NMR spectroscopy and sugar analyses revealed a relatively high content of unsubstituted xylose residues (48–58%) as well as a relatively high content of doubly substituted xylose residues (28–33%) in the structure of the arabinoxylans. β‐Glucans constituted a minor portion of water‐extractable malt polysaccharides and were characterized by high levels of tri‐ and tetrasaccharide residues (93.4%) with a molar ratio of 2.19 for cellotriosyl to cellotetraosyl units. Size‐exclusion chromatography revealed that the WE material contained several polymer populations. One population had a very high molecular weight that appeared to be the result of aggregation. The AX fraction contained higher molecular weight polymers than the BG fraction.
Differences Between Sensory Profiles and Development of Rancidity During Long‐Term Storage of Native and Processed Oat
Authors: Heiniö R.‐L. , Lehtinen P. , Oksman‐Caldentey K.‐M. , Poutanen K. 
Published: June 2007

ABSTRACTChanges in the sensory attributes, lipid composition, and amounts of volatile and phenolic compounds of native and processed (germinated and dried) crushed oat were followed during a 12‐month storage period. The influence of the chemical attributes on the sensory profiles of oats was analyzed by statistical multivariate techniques (PLS regression). During the storage period, significant changes in the sensory profiles of the native and processed oat groats were observed. The stability of oat groats was significantly increased through germination and subsequent drying because the chemical changes causing rancidity and bitterness developed more slowly in the processed oat when compared with the native oat. In native oat, the most intensive changes due to deteriorat ion had already occurred after one month of storage, whereas in processed oat, these changes were perceived considerably later. Stored oat that had deteriorated was evaluated as being musty and earthy in odor and bitter and rancid in flavor. The accumulation of free fatty acids and volatile compounds related to lipid oxidation were closely correlated with the development of the undesired sensory attributes described above. The total amount of phenolic compounds, as well as the volatile aromatic and branched chain compounds derived mainly from protein degradation, showed a significant relationship with favorable sensory attributes such as roasted odor and flavor. Lipid oxidation occurred during the storage and was observed both in the polar and in the nonpolar lipid classes of native oat, whereas in the processed oat, these changes were nonsignificant. Photo‐oxidation of acylated fatty acids may significantly contribute to the development of volatile lipid oxidation products during storage.