bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of plasma inflammatory markers in late-life depression and conversion to dementia: a 3-year follow-up study
Authors: Bocharova M. , Borza T. , Watne L. O. , Engedal K. , O’Brien J. T. , Selbæk G. , Idland A. V. , Hodsoll J. , Young A. H. , Aarsland D. 
Published: February 2025

Abstract
Late-life depression (LLD) has been linked to increased likelihood of dementia, although mechanisms responsible for this association remain largely unknown. One feature frequently observed in both LLD and dementia is elevated levels of plasma inflammatory markers. The present study aimed to compare the levels of 12 plasma inflammatory markers between older people with LLD and controls, and to explore whether these markers, along with clinical characteristics, can predict dementia in patients with LLD within 3 years of follow-up. Using multiple linear regression with stepwise adjustment, we compared levels of plasma inflammatory markers (IL-1β, IL-1ra, IL-6, IL-10, IL-17a, IL-18, IL-33, TNFα, CD40L, IFN-γ, CCL-2 and CCL-4) between 136 inpatients with LLD (PRODE cohort) and 103 cognitively healthy non-depressed controls (COGNORM cohort). In the PRODE cohort, follow-up data was available for 139 patients (of them 123 had data on baseline plasma inflammatory markers); 36 (25.9%) developed dementia by Year 3 (n = 31 for those with cytokine data). Using Cox proportional hazards regression, we explored whether inflammatory markers and clinical characteristics of LLD (age of onset, treatment response, number of episodes) predicted progression to dementia during follow-up. Levels of IL-1ra, CCL-2, CCL-4, IFN-γ and IL-17a were significantly higher in LLD patients compared to controls in the majority of models. However, none of the inflammatory markers predicted progression from LLD to dementia in the PRODE cohort. Among clinical features, only poor response to treatment significantly predicted higher risk of progression to dementia.
Generation of PLASE score for patent ductus arteriosus using the PLASE study database
Authors: Masutani Satoshi , Isayama Tetsuya , Kobayashi Tohru , Pak Kyongsun , Tomotaki Seiichi , Iwami Hiroko , Yokoyama Takehiko , Toyoshima Katsuaki ,   On behalf of the PLASE (Patent ductus arteriosus and Left Atrial Size Evaluation in preterm infants) study group investigators,   Steering committee, Nagasawa Hiroyuki , Kawasaki Hidenori ,   PLASE study office,   Data coordinating center, Su Chemin ,   Statistical center, Inoue Eisuke , Mikami Masashi ,   Clinical investigators, Matsumura Shun , Nakayama Atsushi , Tachibana Takashi , Toyoshima Katsuaki , Ikeda Toshifumi , Okada Naoko , Okazaki Kaoru , Yamamoto Yutaka , Honda Akane , Matsumoto Atsushi , Amari Shoichiro , Masumoto Kenichi , Onishi Satoshi , Iwami Hiroko , Yamaura Miki , Sato Masahiko , Shiraishi Jun , Kamamoto Tomoyuki , Nakata Yusei , Nii Mitsumaro , Aoyama Aiko , Yamamoto Masahito , Kitano Hiroyuki , Sugiura Hiroshi , Yanagi Takahide , Saito Tomoko , Iwashima Satoru , Ishikawa Takamichi , Hayashi Tomohiro , Ishii Hiroki , Suganami Yusuke , Tanaka Kenichi , Nakao Atsushi , Kobayashi Masaki , Miyata Masafumi , Sato Yoshiaki , Yutaka Nanae , Ohashi Atsushi , Fuke Tomoko , Miwa Masayuki , Kato Takenori , Kato Shin 
Published: February 2025

Abstract

Background
No echocardiographic model, to the best of our knowledge, has been established to predict the future need for patent ductus arteriosus (PDA) surgery. This study aimed to develop a novel predictive score (PLASE score) for anticipating the need for PDA surgery using the PLASE study database.

Methods
The included infants with gestational age (GA) < 30 weeks were allocated to derivation and validation groups (2:1). Logistic regression models were constructed to predict the future need for PDA surgery utilizing three clinical and three echocardiographic indices measured at 3 days of age as candidate variables. ROC-AUCs and 95% confidence intervals (CIs) were obtained by 3-fold cross-validation and the percentile method, respectively. The model with the largest ROC-AUC was tested in the validation data.

Results
Derivation and validation data included 463 and 229 patients, respectively, with 55 and 22 surgical cases, respectively. The ROC-AUC was maximized in the model using GA and all three echocardiographic indices (0.846 [95% CI, 0.805–0.886]). In the validation data, the ROC-AUC for the same model was 0.827 (0.744–0.911).

Conclusions
We created a surgical prediction model using simple indices at 3 days of age, and the validation data demonstrated good predictive ability.

Impact

No early predictive model has been established for the future need of patent ductus arteriosus (PDA) surgery in preterm infants.

A new prediction model was created with the Patent ductus arteriosus and Left Atrial Size Evaluation study in preterm infants (PLASE) database (N = 692), incorporating gestational age and three simple echocardiographic indices measured at 3 days of age. The model demonstrates high discrimination and calibration.

This model provides risk stratification for preterm PDA and may contribute to early preterm management.
Normative data for dimensions of the frontal and infraorbital nerves
Authors: Tong Jessica Y. , Sung Jeffrey , Rana Khizar , Chan WengOnn , Psaltis Alkis J. , Selva Dinesh 
Published: February 2025

Abstract

Objectives
To present a series of normative measurements for the width of the frontal and infraorbital nerve branches of V1 and V2, respectively.

Methods
Cadaveric dissection study of 15 embalmed cadaver heads (30 orbits). The frontal nerve was excised en bloc from the superior orbital rim to the superior orbital fissure. Similarly, the infraorbital nerve was excised en bloc from the inferior orbital rim to the orbital apex. Measurements were recorded of the maximal width of the frontal nerve, infraorbital nerve within the orbital floor, and pterygopalatine segment of the maxillary nerve. Any value greater than 2 standard deviations (SD) above the mean value, was defined as nerve enlargement.

Results
The mean transverse diameter of the frontal nerve was 2.27 ± 0.66 mm (1 SD). The mean transverse diameter of the infraorbital nerve branch, and the maxillary nerve within the pterygopalatine fossa, was 3.31 ± 0.68 mm (1 SD) and 3.59 ± 0.76 mm (1 SD), respectively. The upper limit of normal, defined as 2 SD above the mean value, for the widths of the frontal nerve, infraorbital nerve, and pterygopalatine segment of V2 widths were 3.59 mm, 4.67 mm, and 5.10 mm, respectively.

Conclusions
The frontal and infraorbital nerves are implicated in various inflammatory and neoplastic orbital pathologies. Defining the normative data for width is important to compare with pathological states.
Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus
Authors: Wu Jiadong , Liu Sijia , Zhang Haoyu , Chen Sisi , Si Jingna , Liu Lin , Wang Yue , Tan Shuxian , Du Yuxin , Jin Zhelun , Xie Jianbo , Zhang Deqiang 
Published: February 2025

Abstract
Plant growth behavior is a function of genetic network architecture. The importance of root microbiome variation driving plant functional traits is increasingly recognized, but the genetic mechanisms governing this variation are less studied. Here, we collect roots and rhizosphere soils from nine Populus species belonging to four sections (Leuce, Aigeiros, Tacamahaca, and Turanga), generate metabolite and transcription data for roots and microbiota data for rhizospheres, and conduct comprehensive multi-omics analyses. We demonstrate that the roots of vigorous Leuce poplar enrich more Pseudomonas, compared with the poorly performing poplar. Moreover, we confirm that Pseudomonas is strongly associated with tricin and apigenin biosynthesis and identify that gene GLABRA3 (GL3) is critical for tricin secretion. The elevated tricin secretion via constitutive transcription of PopGL3 and Chalcone synthase (PopCHS4) can drive Pseudomonas colonization in the rhizosphere and further enhance poplar growth, nitrogen acquisition, and secondary root development in nitrogen-poor soil. This study reveals that plant-metabolite-microbe regulation patterns contribute to the poplar fitness and thoroughly decodes the key regulatory mechanisms of tricin, and provides insights into the interactions of the plant’s key metabolites with its transcriptome and rhizosphere microbes.
Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing
Authors: Yu Lisha , Liu Zhaodi , Zheng Yong , Tong Zongrui , Ding Yihang , Wang Weilin , Ding Yuan , Mao Zhengwei 
Published: February 2025

Abstract
Uncontrolled haemorrhage is a leading cause of trauma-related fatalities, highlighting the critical need for rapid and effective haemostasis. Current haemostatic materials encounter limitations such as slow clotting and weak mechanical strength, while most of bioadhesives compromise their adhesion performance to wet tissues for biocompatibility and degradability. In this study, a molecular self-assembly strategy is proposed, developing a biocompatible and biodegradable protein-based patch with excellent adhesion performance. This strategy utilizes fibrinogen modified with hydrophobic groups to induce self-assembly into a hydrogel, which is converted into a dry patch. The protein patch enhances adhesion performance on the wet tissue through a dry cross-linking method and robust intra/inter-molecular interactions. This patch demonstrates excellent haemostatic efficacy in both porcine oozing wound and porcine severe acute haemorrhage. It maintains biological functionality, and ensures sustained wound sealing while gradually degrading in vivo, making it a promising candidate for clinical tissue sealing applications.
Comparative performance of tuberculin and defined-antigen cocktails for detecting bovine tuberculosis in BCG-vaccinated cattle in natural settings
Authors: Fromsa Abebe , Conlan Andrew J. K. , Srinivasan Sreenidhi , Gumi Balako , Bedada Wegene , Zeleke Miserach , Worku Dawit , Lakew Matios , Tadesse Biniam , Bayissa Berecha , Sirak Asegedech , Abdela Musse Girma , Mekonnen Getnet Abie , Chibssa Tesfaye , Veerasami Maroudam , Jones Gareth J. , Vordermeier H. Martin , Juleff Nick , Wood James L. N. , Ameni Gobena , Kapur Vivek 
Published: February 2025

Abstract
Bovine tuberculosis (bTB) is a threat to cattle health and public safety. The current control programs are hampered by wildlife reservoirs and socioeconomic barriers. Vaccinating cattle with Bacillus Calmette-Guérin (BCG) effectively reduces transmission, offering a potential solution for controlling bTB. A key requirement for vaccination strategies using BCG is the validation of defined antigens to differentiate infections among vaccinated animals (DIVA). We compared tuberculin with DIVA peptide cocktails (ESAT-6, CFP-10, and Rv3615c) in 67 unvaccinated and 67 BCG-vaccinated cattle exposed to M. bovis in a natural setting. The cattle were tested every 4 months with a skin test and every 2 months with interferon-gamma (IFN-γ) release assays (IGRA) over a year of exposure. Before exposure, the DIVA skin, DIVA IGRA, and tuberculin tests showed 100% specificity in unvaccinated control calves. After exposure, the DIVA skin, DIVA IGRA, and comparative cervical tuberculin (CCT) tests had comparable sensitivities of 46% (95% CI 36, 56), 45% (95% CI 35, 55), and 47 (95% CI 37, 57), respectively, when assessed against animals positive by M. bovis culture PCR. The results suggest that test-and-slaughter control strategies using tests with low sensitivity are not expected to be effective in controlling bTB in high-prevalence herds, and highlight an urgent need to improve the sensitivity of diagnostic tests for bTB in these settings.
Direct current electrical fields inhibit cancer cell motility in microchannel confinements
Authors: Naggay Benjamin Karem , Farahani Saeed Khomeijani , Gao Xu , Holle Andrew , Kemkemer Ralf 
Published: February 2025

Abstract
The capability of cells to sense and respond to endogenous electrical fields plays a crucial role in processes like nerve regeneration, wound healing, and development. In vitro, many cell types respond to electrical fields by migrating along the corresponding electrical field vectors. This process is known as galvano- or electrotaxis. Here we report on the combined impact of micro-confinements and direct current electrical fields (dcEFs) on the motility of MDA-MB-231 human breast cancer cells using a self-developed, easy-to-use platform with microchannels ranging from 3

$$\upmu$$

μ

m to 11

$$\upmu$$

μ

m in width and 11

$$\upmu$$

μ

m height. We found that MDA-MB-231 cells respond to exogenous electrical fields ranging from 100 mV mm

$$^{-1}$$

-
1

to 1000 mV mm

$$^{-1}$$

-
1

with altered cell motility depending on the confinement size. Our data show an overall inhibited galvanotaxis in confinements, while in contrast an enhancing effect in unconfined galvanotaxis is found. The application of direct current electrical fields to microchannels not only caused a reduction in migration speed but also decreased the number of permeating cells. By applying 1000 mV mm

$$^{-1}$$

-
1

, single-cell permeation could be prevented in confinements of 5

$$\upmu$$

μ

m and smaller.
Associations between nonrestorative sleep and suicidal ideation: a Japanese general population survey
Authors: Yamada Kouju , Kaneko Yoshiyuki , Konno Chisato , Furihata Ryuji , Otsuka Yuichiro , Kaneita Yoshitaka , Uchiyama Makoto , Suzuki Masahiro 
Published: February 2025

Abstract
Insomnia symptoms and short sleep duration are known to be associated with suicidal ideation. The aim of this study was to investigate the association between nonrestorative sleep (NRS), a subjective experience of feeling unrefreshed after waking up, and suicidal ideation in the general population. We analyzed data from a cross-sectional survey of 2559 randomly sampled adults living in Japan. The participants were asked about insomnia symptoms (difficulty initiating sleep, difficulty maintaining sleep, and early morning awakening), NRS, sleep duration, and the presence of suicidal ideation. Logistic regression analysis was used to examine the associations among short sleep duration (< 6 h), insomnia symptoms, NRS, and suicidal ideation. Possible confounding factors were adjusted using propensity scores. In the multivariate analysis adjusting for the confounding effects of other sleep-related factors, in addition to psychiatric, sociodemographic, and lifestyle factors, only NRS showed a positive association with suicidal ideation (adjusted odds ratio [aOR] = 2.266). These findings suggest the importance of focusing on NRS for suicide prevention in the general population.
The effects of Thymus capitatus essential oil topical application on milk quality: a systems biology approach
Authors: Nehme Ralph , Gini Chiara , Vanbergue Elise , Even Sergine , Biscarini Filippo , Andrés Sonia , Rault Lucie , Noel Faustine , Hardit Valerie , Bouhallab Said , Pereira David M. , Ksouri Riadh , Roussel Philippe , López Secundino , Cremonesi Paola , Castiglioni Bianca , Caruso Donatella , Faré Fiorenza , Fontana Manuela , Ceciliani Fabrizio , Abdennebi-Najar Latifa 
Published: February 2025

Abstract
Essential oils (EO) are known for their antibacterial and anti-inflammatory properties and can be used as an alternative to reduce the reliance on antimicrobials in dairy cattle. While many studies have explored the beneficial properties of EO in vitro, their effects on milk quality and milk microbiota, when applied directly to the udder skin, remain relatively unknown. This study aimed to investigate the impact of Thymus capitatus essential oil (TCEO), known for its high antibacterial and antioxidant properties, on milk microbiota using 16S rRNA sequencing, the lipidomic profile via liquid chromatography-quadrupole time-of-flight mass spectrometry, udder skin microbiota, and inflammatory biomarkers of dairy cows at the end of lactation. Sixteen-quarters of 12 Holstein cows were selected, and TCEO was topically applied to the udder skin twice a day for 7 days. Milk was collected aseptically on days 0, 7, 21, and 28 before morning farm milking. The results showed no significant changes in microbiota composition after the EO treatment in alpha and beta diversity or taxonomical composition at the phylum and genus levels. TCEO induced limited changes in the milk lipidome, primarily affecting diacylglycerols at T21. The treatment did not affect inflammatory biomarkers, milk sensory properties, or quality. Our study is the first to demonstrate that a local application of 10% TCEO on cow’s quarters does not significantly alter milk quality or microbiota composition in milk and skin. More studies should be conducted to ensure the safe use of TCEO in dairy cows and explore its potential benefits on antibiotic-resistant bacteria as an alternative or support for antibiotic therapy.
Tunnel face stability in strata rich in water with soft upper and hard lower layers
Authors: Zhan Tao , Hu Ziwei , Kuang Yan , Zhu Zhiliang , Liao Kaiyi 
Published: February 2025

Abstract
When a shield tunnel is excavated in water-rich strata with soft upper and hard lower layers, the failure mode of the tunnel face may shift from an overall failure mode to a partial failure mode. To address this issue, three-dimensional discrete failure mechanisms for both partial and overall failure modes are established on the basis of the upper bound theorem and spatial discretization techniques. The influence of pore water pressure is also considered, leading to the development of a method for calculating the critical support force of tunnel faces in such strata, while taking into account both failure modes and the effects of groundwater. Through parameter analysis, factors such as the tunnel diameter, proportion of soft soil, stratum cohesion, and pore water pressure coefficient significantly influence the tunnel face failure mode. A comprehensive critical support force, which takes both partial and overall failure modes into account, is proposed. The parameter analysis reveals that this comprehensive critical support force exhibits complex variations under the influence of multiple parameters. At the same time, a method is proposed to determine the upper and lower bounds of the ultimate support force, based on the calculation results under no seepage and free seepage conditions at the excavation face. The entire method provides a valuable reference for the stability analysis of tunnel faces in water-rich strata with soft upper and hard lower layers.
Characterization of paleodrainages in desert regions of Saudi Arabia multisatellite images with field based study
Authors: Al Saud Mashael M. 
Published: February 2025

Abstract
In Saudi Arabia, a number of linear geomorphological features with uncertain origin have been observed from space, but they do not belong to any existed drainage systems. They are ancient watercourses carried water in the past during the Holocene deluge, and they were affected by global climate change and geological processes turning them into dry and buried channels filled by sediments, and these are described as “Paleodrainages”. This study investigated these features primarily based on the integration of multi-satellite images including SRTM DEM for generating stream networks, ALOS-PALSAR which is capable to penetrate the surficial materials, and ASTER for detecting thermal differentiation in terrain surface. The novelty of this study includes the use of more than one satellite images (optical and microwave) with various spectral and optical characteristics, and this has been supported by field verification to investigate the lithological facies of stuffed materials into the detected paleodrainages, plus the classification performed for the detected paleodrainages and this has never been implemented in previous studies. Hydro-geomorphological-based categorization of these paleodrainages was carried out, indicating the majority of SW-NE trending and the presence of routes, unconsolidated sediments and rocks. From the hydrological point of view, these paleodrainages are potential for groundwater storage; and they can be also suitable sites for artificial groundwater recharge; be-sides they represent routs for saltwater intrusion on-land; and these science-based clues represent supportive element for better water resources management in Saudi Arabia.
Networked disobedience to smart city development: The case of Hong Kong
Authors: Ting Tin‐Yuet 
Published: February 2025

AbstractWhen urban landscapes erupt into civil unrests, smart technologies that are intended to help preserve social order may become prime sites of contention. Integrating critical data studies and research on networked social movements, this article examines the underexplored contours of networked disobedience to smart city development – that is, direct action by self‐mobilised and self‐organised digitally connected citizens and activists to subvert or disrupt the dominant structure of the datafied smart city – during a large‐scale protest movement. The case of Hong Kong's smart lampposts is analysed to explicate a distinct technopolitical contention that emerged in the digital age, focusing on three key aspects: (1) citizens' digital curation of folk theories, which perpetuated a consensus of discontent over the installation of smart city technology, (2) the articulation of a digitised network of counter‐power that provided a mediation opportunity structure for mobilisation and intervention, and (3) the crowdsourcing of disobedient practices of data activism aimed at sabotaging or evading the smart city technology. The article illustrates how seemingly ordinary issues of urban datafication can be repurposed to (re)produce political contention and the ways in which controversies over smart city development may fuel adversarial citizen–state engagement with repercussions for data‐driven urban governance.
Is there a need for a CT scan of the pancreatic phase? A perfusion and simulation study of the pancreas, an HCC, and the kidney cortex
Authors: Cressoni Massimo , Cadringher Paolo , Colarieti Anna , Darvizeh Fatemeh , Cozzi Andrea , Cina Claudio , Zanardo Moreno , Ambrogi Federico , Ippolito Davide , Sardanelli Francesco 
Published: February 2025

AbstractTo explore the peak enhancement time of a hepatocellular carcinoma, the pancreas, and the kidney cortex and its determinants.We obtained a time enhancement curve from the perfusion CT scans of 11 advanced HCC patients (40 volumes at 1.25 s time interval, slab slice 90 mm, bolus of 50 ml of iodinated contrast agent, 350 g iodine/ml, flow 5 ml/s). Small regions of interest were drawn on the abdominal aorta, the HCC, the cortex of the right kidney, and on the pancreas. The behavior of the contrast agent in the capillary and in the surrounding tissue was further explored with a finite element model.The peak enhancement time of the pancreas did not differ from that of the HCC (10±3 vs. 11±4 s, p=0.9), while the peak enhancement time of the kidney tended to be a few seconds earlier (8±1 s, p=0.082 vs. pancreas and p=0.069 vs. kidney). Simulation showed that the time span in which the tissue enhancement remained within 10% of its peak value was similar across all capillary densities and ranged between 26−38 s for a capillary density of 0.00125 per mm to 30–60 s for a capillary density of 0.01.The plateau tissue enhancement clinically acquired in the “late arterial phase” should be adequate both for the detection of hypervascular liver lesions such as HCCs and for obtaining peak pancreatic enhancement to detect hypovascular lesions.