bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preoperative 11C-Methionine PET-MRI in Pediatric Infratentorial Tumors
Authors: Beuriat Pierre-Aurélien , Flaus Anthime , Portefaix Aurélie , Szathmari Alexandru , Janier Marc , Hermier Marc , Lorthois-Ninou Sylvie , Scheiber Christian , Isal Sibel , Costes Nicolas , Merida Ines , Lancelot Sophie , Vasiljevic Alexandre , Leblond Pierre , Faure Conter Cécile , Saunier Clarisse , Kassai Behrouz , Vinchon Matthieu , Di Rocco Federico , Mottolese Carmine 
Published: March 2024

Purpose
MRI is the main imaging modality for pediatric brain tumors, but amino acid PET can provide additional information. Simultaneous PET-MRI acquisition allows to fully assess the tumor and lower the radiation exposure. Although symptomatic posterior fossa tumors are typically resected, the patient management is evolving and will benefit from an improved preoperative tumor characterization. We aimed to explore, in children with newly diagnosed posterior fossa tumor, the complementarity of the information provided by amino acid PET and MRI parameters and the correlation to histopathological results.

Patients and Methods
Children with a newly diagnosed posterior fossa tumor prospectively underwent a preoperative 11C-methionine (MET) PET-MRI. Images were assessed visually and semiquantitatively. Using correlation, minimum apparent diffusion coefficient (ADCmin) and contrast enhancement were compared with MET SUVmax. The diameter of the enhancing lesions was compared with metabolic tumoral volume. Lesions were classified according to the 2021 World Health Organization (WHO) classification.

Results
Ten children were included 4 pilocytic astrocytomas, 2 medulloblastomas, 1 ganglioglioma, 1 central nervous system embryonal tumor, and 1 schwannoma. All lesions showed visually increased MET uptake. A negative moderate correlation was found between ADCmin and SUVmax values (r = −0.39). Mean SUVmax was 3.8 (range, 3.3–4.2) in WHO grade 4 versus 2.5 (range, 1.7–3.0) in WHO grade 1 lesions. A positive moderate correlation was found between metabolic tumoral volume and diameter values (r = 0.34). There was no correlation between SUVmax and contrast enhancement intensity (r = −0.15).

Conclusions
Preoperative 11C-MET PET and MRI could provide complementary information to characterize pediatric infratentorial tumors.
ATF4 orchestrates IL-1α-induced senescence in adult neural stem cells
Authors: Neumann Susanne , Thelin Eric P. , Sankavaram Sreenivasa Raghavan , Persson Sanna , Fonseca Leonor , Moruzzi Noah , Iacobaeus Ellen , Bergsland Maria , Popova Elena , Bader Michael , Svensson Mikael , Espinosa Alexander , Covacu Ruxandra , Brundin Lou 
Published: March 2024

AbstractAdult neural stem cells (NSC) are a potential source for the regeneration of damaged tissue during neuropathological conditions, but much remains unexplored. In an attempt to study the influence of neuroinflammation on NSCs, we generated a transgenic reporter rat strain that expresses theDiscosoma sp. red (DsRed) fluorophore in NSCs and subjected it to traumatic brain injury (TBI). Transcriptomic analysis of NSCs isolated from TBI revealed an enrichment of stress response genes that pertained to endoplasmic reticulum (ER) stress and integrated stress response (ISR). Downstream analysis on NSC cultures pinpointed IL-1α as a trigger of ISR in these cells. At concentration levels similar to the ones measured post-TBI in rats, IL-1α induced the translation of activating transcription factor 4 (ATF4), an ISR master regulator. Further, ATF4 was necessary for the IL-1α -dependent induction of a senescent profile in NSCs, which included a metabolic shift towards glycolysis, induction of senescence-associated secretory phenotype, SASP, and cell cycle arrest. In summary, the ISR/ATF4 pathway seems to play a major role in NSC function during neuroinflammation and provides a therapeutic tool for protecting the NSC pool during these conditions.
Diffusion and Oligomerization States of the Muscarinic M1 Receptor in Live Cells − The Impact of Ligands and Membrane Disruptors
Authors: Zhou Xiaohan , Septien-Gonzalez Horacio , Husaini Sami , Ward Richard J. , Milligan Graeme , Gradinaru Claudiu C. 
Published: March 2024

ABSTRACTG protein-coupled receptors (GPCRs) are a major gateway to cellular signaling, which respond to ligands binding at extracellular sites through allosteric conformational changes that modulate their interactions with G proteins and arrestins at intracellular sites. High-resolution structures in different liganded states together with spectroscopic studies and molecular dynamics simulations have revealed a rich conformational landscape of GPCRs. However, their supramolecular spatio-temporal distribution is also thought to play a significant role in receptor activation and signaling bias within the native cell membrane environment. Here, we applied single-molecule fluorescence techniques, including single-particle tracking, single-molecule photobleaching and fluorescence correlation spectroscopy, to characterize the diffusion and oligomerization behavior of the muscarinic M1receptor (M1R) in live cells. Control samples included the monomeric protein CD86 and fixed cells, and experiments performed in the presence of different orthosteric M1R ligands and of several compounds known to change the fluidity and organization of the lipid bilayer. M1receptors exhibit Brownian diffusion characterized by three diffusion constants:confined/immobile(∼0.01 μm2/s),slow(∼0.04 μm2/s), andfast(∼0.14 μm2/s), whose populations were found to be modulated by both orthosteric ligands and membrane disruptors. The lipid raft disruptor C6-ceramide led to significant changes for CD86 while leaving the diffusion of M1R unchanged, indicating that M1receptors do not partition in lipid rafts. The extent of receptor oligomerization was found to be promoted by increasing the level of expression, and the binding of orthosteric ligands; in particular the agonist carbachol elicited a large increase in the fraction of M1R oligomers. This study provides new insights into the balance between conformational and environmental factors that define the movement and oligomerization states of GPCRs in live cells under close-to-native conditions.
Twist - torsion coupling in beating axonemes
Authors: Striegler Martin , Friedrich Benjamin M. , Diez Stefan , Geyer Veikko F. 
Published: March 2024

AbstractMotile cilia and flagella are ubiquitous cell appendages whose regular bending waves pump fluids across tissue surfaces and enable single-cell navigation. Key to these functions are their non-planar waveforms with characteristic torsion. It is not known how torsion, a purely geometric property of the shape, is related to mechanical deformations of the axoneme, the conserved cytoskeletal core of cilia and flagella. Here, we assess torsion and twist in reactivated axonemes isolated from the green algaChlamydomonas reinhardtii. Using defocused darkfield microscopy and beat-cycle averaging, we resolve the 3D shapes of the axonemal waveform with nanometer precision at millisecond timescales. Our measurements reveal regular hetero-chiral torsion waves propagating base to tip with a peak-to-peak amplitude of 22 º/µm. To investigate if the observed torsion results from axonemal twist, we attach gold nanoparticles to axonemes to measure its cross-section rotation during beating. We find that locally, the axonemal cross-section co-rotates with the bending plane. This co-rotation presents the first experimental evidence for twist-torsion coupling and indicates that twist waves propagate along the axoneme during beating. Our work thus links shape to mechanical deformation of beating axonemes, informing models of motor regulation that shape the beat of motile cilia.
Understanding switching intentions between traditional banks and Internet-only banks among Generation X and Generation Z
Authors: Park Junsung , Yoo Joon Woo , Cho Youngju , Park Heejun 
Published: March 2024

PurposeThis study aims to understand the reasons for individuals switching from traditional banks to Internet-only banks and examine how switching intentions differ between Generation X and Generation Z. Notably, Generation Z, being digital natives, exhibits distinct characteristics compared to Generation X, who often referred to as digital immigrants. Given the technology-driven nature of Internet-only banks, a multi-group analysis between these two generations was conducted.Design/methodology/approachThis study utilizes Bansal’s push–pull–mooring model as a framework to analyze switching intention. The study collected survey data from 383 Korean participants, consisting of 198 participants from Generation Z and 185 participants from Generation X.FindingsThe findings indicate that low satisfaction and discomfort are factors that push people to leave traditional banks. Specifically, Generation Z shows a significantly higher inclination to leave traditional banks due to discomfort. On the other hand, relative advantage, compatibility, observability and trialability are factors that pull people to switch to Internet-only banks. Generation X is more likely to consider adopting Internet-only banks when compatibility is high and complexity is low.Originality/valueThis study is the first to explore unique motivators for Generation Z, such as their discomfort with interpersonal interactions in the retail banking sector. These findings challenge earlier research emphasizing human interaction’s importance in technology adoption, offering insights into their future adoption of contactless services.
Youth mentors’ experiences working with young people to support desistance from criminal behaviour within the community
Authors: Newton Abigail , Robson Megan , Johnson Darren 
Published: March 2024

Purpose
Young offender mentoring programmes aim to support young people’s desistance from offending, but despite the importance, there remains limited exploration into mentor experiences of supporting the young people. This study aims to explore how a community-based mentoring intervention supports desistance in young offenders by understanding the mentor's experiences, with a specific reflective focus on facilitators and barriers to their work.

Design/methodology/approach
Semi-structured interviews were conducted with seven mentors from Northumbria Coalition against Crime, a youth and community service. Interview transcripts were analysed using interpretative phenomenological analysis, with external auditing conducted by the research supervisor.

Findings
Two superordinate themes resulted: “Factors for engagement” and “Personal experiences”, with participant disclosures reflecting professional reward and a sense of success. This was interwoven with “burnout”, emotional investment and challenges linked to barriers to effectiveness. Challenges included the young people having external negative influences, multiple individuals involved in a person’s care and the barrier of in person activities during the coronavirus pandemic. The clinical importance of mentoring programmes, implications for future working practice and research limitations are considered.

Practical implications
The clinical importance of mentoring programmes, implications for future working practice and research limitations are considered.

Originality/value
These findings contribute to understanding mentors’ experiences of working with young people in the community, offering critical insight into the mentorship and wider service dynamics. Furthermore, it provides an inaugural evaluation of the Northumbria Coalition against Crime services.
Exploring the fear of missing out (FOMO) and customer bonds in service relationships
Authors: Harrison-Walker L. Jean , Mead James A. 
Published: March 2024

Purpose
Most research has investigated the fear of missing out (FOMO) in the context of online activities, often associated with negative personal outcomes such as fatigue and stress. However, given the increased desire to be informed and included associated with FOMO, organizations that can effectively meet these needs may develop or strengthen social and structural bonds, thereby turning short-term customers with FOMO into lifelong patrons. This study aims to examine the relationship between FOMO and favorable organizational outcomes as mediated by several constructs associated with the desire for information and inclusion.

Design/methodology/approach
This research was conducted within the higher education sector of the service industry. FOMO served as the IV. The mediators represented context-specific aspects of campus involvement and inclusion. Organizational outcomes related to the long-term services relationship served as the DVs. The sample consisted of 435 students recruited from research pools at two southern universities in the USA. Exploratory factor analysis, OLS regression and the Hayes–Macro were used to examine the data.

Findings
The results demonstrate that FOMO is positively associated with students’ desires for information and inclusion (informal peer interaction, campus involvement, informal faculty interaction, campus information media use and a preference for in-person course scheduling), which are associated with the desirable university outcomes of satisfaction, connection and alumni donation/activity intentions.

Practical implications
If a university fosters unstructured time spent with faculty and peers, and promotes campus information media involvement, students with higher levels of FOMO are more likely to be satisfied, feel connected to the university and report intentions to donate time and money as alumni.

Originality/value
Prior research on FOMO is generally focused on internet and social media use; this study takes a broader perspective and identifies the effect of FOMO on a desire for information and inclusion within a novel context (a service environment). It also associates FOMO with favorable long-term service relationship outcomes that fortify social and structural bonds.
Analysis of wheel-rail adhesion redundancy considering the third-body medium on the rail surface
Authors: Tian Chun , Zhai Gengwei , Wu Mengling , Zhou Jiajun , Li Yaojie 
Published: March 2024

PurposeIn response to the problem of insufficient traction/braking adhesion force caused by the existence of the third-body medium on the rail surface, this study aims to analyze the utilization of wheel-rail adhesion coefficient under different medium conditions and propose relevant measures for reasonable and optimized utilization of adhesion to ensure the traction/braking performance and operation safety of trains.Design/methodology/approachBased on the PLS-160 wheel-rail adhesion simulation test rig, the study investigates the variation patterns of maximum utilized adhesion characteristics on the rail surface under different conditions of small creepage and large slip. Through statistical analysis of multiple sets of experimental data, the statistical distribution patterns of maximum utilized adhesion on the rail surface are obtained, and a method for analyzing wheel-rail adhesion redundancy based on normal distribution is proposed. The study analyzes the utilization of traction/braking adhesion, as well as adhesion redundancy, for different medium under small creepage and large slip conditions. Based on these findings, relevant measures for the reasonable and optimized utilization of adhesion are derived.FindingsWhen the third-body medium exists on the rail surface, the train should adopt the low-level service braking to avoid the braking skidding by extending the braking distance. Compared with the current adhesion control strategy of small creepage, adopting appropriate strategies to control the train’s adhesion coefficient near the second peak point of the adhesion coefficient-slip ratio curve in large slip can effectively improve the traction/braking adhesion redundancy and the upper limit of adhesion utilization, thereby ensuring the traction/braking performance and operation safety of the train.Originality/valueMost existing studies focus on the wheel-rail adhesion coefficient values and variation patterns under different medium conditions, without considering whether the rail surface with different medium can provide sufficient traction/braking utilized adhesion coefficient for the train. Therefore, there is a risk of traction overspeeding/braking skidding. This study analyzes whether the rail surface with different medium can provide sufficient traction/braking utilized adhesion coefficient for the train and whether there is redundancy. Based on these findings, relevant measures for the reasonable and optimized utilization of adhesion are derived to further ensure operation safety of the train.
Touch sensors used in Industry 4.0 to machines in the manufacturing industry on occupational health and safety
Authors: Ağseren Serkan , Şimşek Süleyman 
Published: March 2024

PurposeThis study aims to prevent occupational accidents occurring in the manufacturing industry by means of touch sensors. When the occupational accidents occurring in the manufacturing industry around the world are examined, it is seen that approximately 88% of occupational accidents occur from “dangerous movement” and 10% from “dangerous situation.” Although some studies related to safety culture studies, safety studies in design and collective or personal protective measures have been started, they have not been brought to an adequate level. It is observed that studies on dangerous movements continue even in many developed countries. In this study, first of all, a literature study was conducted. Occupational accidents experienced in the manufacturing sector in Turkey have been examined. In line with these investigations, a prototype circuit protection system has been developed that can prevent accidents caused by dangerous movement. With the circuit, its applicability and effectiveness were measured by conducting experiments on different manufacturing machines. The prototype circuit applied in this paper was made based on the logic of protective measures made on sawstop machines used in different sectors. In the experimental study conducted, it was observed that in 30 experiments conducted with a prototype on ten separate manufacturing machines, it stopped the machines 26 times at minimum and 29 times at maximum. On average, when looking at the system efficiency values, it was seen that the system was 81.6% effective, and it was observed that positive results could be obtained when converted into a real product.Design/methodology/approachIn this study, their contribution to the prevention of work accidents caused by presses and rotary accents from machines used in the manufacturing industry by means of touch sensors used in Industry 4.0 was examined.FindingsWith Industry 4.0, different automation systems began to be switched in many areas and sectors. Studies have started on different sensors used also in Industry 4.0 in occupational health and safety studies, but it is seen that they have not been applied at an adequate level. It should be designed in such a way as to prevent errors or stop these errors in the studies performed. Today, sensors are produced at much lower costs than before. In addition, the constantly developing technology provides great convenience for these applications.Research limitations/implicationsThis study was applied for press and cylinder machines from manufacturing machines. This study has been tried for machines producing a maximum pressure of 300 tons.Originality/valueA prototype was designed. Trials were done on some machines by prototype. There could be improve and find different solutions for safety problems in the industry with this perspective.
What priorities for change are we missing in inclusion? Symphony of voices with participatory interviews
Authors: Fiuza Asorey María José , Sierra Martínez Silvia , Castro Pais María Dolores , Parrilla Latas Ángeles 
Published: March 2024

AbstractAn interpretative qualitative research project has been carried out in north‐west Spain (Galicia) with the aim of identifying the priorities which, in the opinion of the different agents involved, constitute the major demands of inclusion for an inclusive social and educational transformation. The data collection technique used was the open interview, through which 44 participants, belonging to different groups (headteachers, teacher‐tutors, specialists, counsellors, families, students, associations and politicians), expressed their experiences in relation to the subject of the study. Content analysis was carried out, through a double coding process combining the deductive and inductive approach, supported by the professional software MAXQDA22. The results reveal three main priorities as the main demands of inclusion in order to move towards inclusive social and educational change: (1) reaching collective agreements, (2) creating micro support networks and (3) strengthening teacher commitment.
Brain‐specific serine/threonine‐protein kinase 1 is a substrate of protein kinase C epsilon involved in sex‐specific ethanol and anxiety phenotypes
Authors: Dugan Michael P. , Maiya Rajani , Fleischer Caleb , Bajo Michal , Snyder Angela E. , Koduri Ashwin , Srinivasan Sathvik , Roberto Marisa , Messing Robert O. 
Published: March 2024

AbstractProtein kinase C epsilon (PKCε) regulates behavioural responses to ethanol and plays a role in anxiety‐like behaviour, but knowledge is limited on downstream substrates of PKCε that contribute to these behaviours. We recently identified brain‐specific serine/threonine‐protein kinase 1 (BRSK1) as a substrate of PKCε. Here, we test the hypothesis that BRSK1 mediates responses to ethanol and anxiety‐like behaviours that are also PKCε dependent. We used in vitro kinase assays to further validate BRSK1 as a substrate of PKCε and used Brsk1−/− mice to assess the role of BRSK1 in ethanol‐ and anxiety‐related behaviours and in physiological responses to ethanol. We found that BRSK1 is phosphorylated by PKCε at a residue identified in a chemical genetic screen of PKCε substrates in mouse brain. Like Prkce−/− mice, male and female Brsk1−/− mice were more sensitive than wild‐type to the acute sedative‐hypnotic effect of alcohol. Unlike Prkce−/− mice, Brsk1−/− mice responded like wild‐type to ataxic doses of ethanol. Although in Prkce−/− mice ethanol consumption and reward are reduced in both sexes, they were reduced only in female Brsk1−/− mice. Ex vivo slice electrophysiology revealed that ethanol‐induced facilitation of GABA release in the central amygdala was absent in male Brsk1−/− mice similar to findings in male Prkce−/− mice. Collectively, these results indicate that BRSK1 is a target of PKCε that mediates some PKCε‐dependent responses to ethanol in a sex‐specific manner and plays a role distinct from PKCε in anxiety‐like behaviour.
Archaeology of psychotropic substances: The problem of analytical detection of ayahuasca
Authors: Greco Enrico , Rivier Laurent , Samorini Giorgio , D'Arienzo Adriana 
Published: March 2024

AbstractThe primary objective of this review is to provide a comprehensive overview of the challenges involved in detecting ancient ayahuasca, a traditional hallucinogenic drink from the Amazon region, which is prepared using Banisteriopsis caapi liana and other plants, by utilizing advanced analytical techniques. The presence of harmine and harmaline in Andean archaeological findings has led certain authors to speculate that the Banisteriopsis liana may have been the source plant responsible for their occurrence. Consequently, the utilization of this liana can be traced back to at least 500 CE. However, a combination of archaeological, ethnobotanical, biochemical and analytical chemistry considerations has rendered uncertain the true origins of the presence of harmine and harmaline in ancient mummies and artifacts. Thus, the archaeological evidence does not convincingly prove the ancient usage of ayahuasca.