bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of a metastasis‐deficient lectin‐resistant human melanoma mutant
Authors: Ishikawa M. , Kerbel R. S. 
Published: February 2007

AbstractA mutant (3S5), almost completely deficient in pulmonary metastatic ability in nude mice, was recently isolated from a human malignant melanoma cell line (MeWo). The WGA‐resistance (WGA) phenotype of 3S5 cells was accompanied by a collateral hypersensitivity to the lectin BSII (Bandeiraea simplicifolia), similar to that observed previously in class‐I non‐metastatic WGAr recessive genetic mutants isolated from a highly metastatic mouse tumor. The lectin‐resistance/sensitivity profile of 355 cells was completely stable in tissue culture for 9 months when the cells were grown in the absence of WGA. Twenty independent clones of 3S5 were analyzed and all manifested a similar lectin‐resistance pattern, and severe deficiency in pulmonary metastatic ability following i.v. or s.c. inoculation, when compared to the parent MeWo cells. Some heterogeneity in metastasis was noted, especially with respect to occasional extrapulmonary spread. The majority of the small number of metastases obtained after i.v. inoculation of 3S5 cells maintained the lectin‐resistance profile and deficient metastatic potential characteristic of 3S5 cells upon re‐inoculation into other nude mice. The results provide further evidence that cell‐surface glycosylation changes can significantly alter metastatic potential, including that of human tumors.
Plasminogen activator and its enhancement in differentiating mouse friend erythroleukemia cells
Authors: Amici Carla , Benedetto Arrigo , Saksela Olli , Salonen Eeva‐Marjatta , Vaheri Antti 
Published: February 2007

AbstractUrokinase‐type plasminogen activator (u‐PA) activity was found in the medium as well as in the lysates of cultured uninduced Friend leukemia (FL) cells. PA activity progressively increased during the cell differentiation induced by dimethyl sulphoxide (DMSO), 4‐hydroxy‐3‐methoxybenzaldehyde or hypoxanthine. Both the differentiation and the enhancement of PA activity in cultures of DMSO‐induced cells were blocked by treating the cells with 1 μM dexamethasone. A highly significant correlation (rs = 0.93) was found between the number of hemoglobinized cells and the rate of PA secretion, indicating that the increase in PA activity coincides with late events of the differentiation process. FL cells specifically adhere to fibronectin‐coated surfaces but tend to lose this property during the differentiation process. Anti‐u‐PA IgG antibodies promoted the attachment of differentiating cells to fibronectin‐coated surfaces, suggesting that u‐PA plays a role in the detachment of FL cells from fibronectin immobilized on the growth substratum.
A monoclonal antibody (Bl2‐10D1) reacting with a bladder‐cancer‐associated antigen
Authors: Longin A. , Hijazi A. , Berger‐Dutrieux N. , Escourrou G. , Bouvier R. , Richer G. , Mironneau I. , Fontaniere B. , Devonec M. , Laurent J. C. 
Published: February 2007

AbstractA hybridoma cell line secreting an IgM monoclonal antibody (MAb) was produced after immunizing a mouse with RT4 cells and a crude suspension of human bladder carcinoma cells (WHO grades II and III TCC). This MAb reacted with RT4 target cells derived from a human transitional bladder cancer but failed to react with a majority of non‐bladder cancer cell lines. Immunohistological studies indicate that this MAb reacts inconstantly with normal bladder: in positive cases only a few superficial cells (5% to 10% umbrella cells) are stained but not intermediate or basal cells of the urothelium. This MAb was evaluated on 118 tumors: it reacted with tumor tissue in a majority of grade 1 (79.5%) and grade II papillary TCC (77.3%), less with grade III papillary TCC (45%) and very rarely with invasive non‐papillary TCC (14%). In cases of flat lesions a strong reactivity of superficial, intermediate and/or basal layer cells was observed in 50% of moderate and severe dysplasia and in all cell layers of carcinomas in situ (CIS)(9/9).
Type of alcoholic beverage and oral cancer
Authors: Kabat Geoffrey C. , Wynder Ernst L. 
Published: February 2007

AbstractThe effect on oral cancer risk of different types of alcoholic beverage was investigated using data from a hospital‐based case‐control study. Owing to the small numbers of subjects drinking one beverage exclusively, it was necessary to classify drinkers as consumers of predominantly beer, wine, or hard liquor (i.e., more than 50% of their whiskey equivalents of alcohol derived from a specific beverage). The number of predominantly wine drinkers was too small to permit analysis. Logistic regression was used to obtain estimates of the risk associated with each predominant beverage, with adjustment for other risk factors and confounding variables. In males, the odds ratio for predominantly beer drinkers increased with increasing level of intake, reaching 4.87 (95% confidence interval: 2.51–9.46) in drinkers of 7 + oz. of whiskey equivalents/day. The odds ratio for predominantly hard liquor drinkers showed a similar increase, reaching 5.74 (95% confidence interval: 2.94–11.22) in predominantly hard liquor drinkers consuming 7+ oz. of whiskey equivalents/day, suggesting that the effect of these 2 major types of alcoholic beverage is of similar magnitude. The trends were less clearcut in women due to small numbers of drinkers.
Abrogated NK‐cell lysis of human papillomavirus (HPV)‐16‐bearing keratinocytes in patients with pre‐cancerous and cancerous HPV‐induced anogenital lesions
Authors: Malejczyk Jacek , Majewski Slawomir , Jablonska Stefania , Rogozinski Tomasz T. , Orth Gerard 
Published: February 2007

AbstractNatural‐cell‐mediated cytotoxicity against K‐562 erythroleukemic cells and human papillomavirus (HPV)‐16 harboring Sk‐v keratinocytes was tested in 38 age‐ and sex‐matched healthy volunteers and in patients with HPV‐induced benign and malignant anogenital lesions: 9 persons with HPV‐16‐induced bowenoid papulosis (BP), 8 with anogenital carcinomas (5 with HPV‐16‐ or 33‐associated squamous‐cell carcinomas of Bowen's type and 3 with HPV‐6‐associated Buschke‐Loewenstein verrucous carcinomas) and 12 with HPV‐6‐induced condylomata acuminata. Both K‐562 and Sk‐v cells were killed by a non‐adherent CD16+ subset of PBMC as revealed by cell fractionation on the basis of their adherence to plastic and by treatment with Leu‐IIb monoclonal antibody (MAb) and complement. “Cold” target competitive assays demonstrated that both cell types inhibited lysis of labelled Sk‐v cells. In patients with BP and anogenital carcinomas induced by HPV‐16 or 33, there was a significant (at least at p < 0.01) decrease of Sk‐v cell lysis as compared with the healthy control group. Anti‐K‐562 activity was not affected. In patients with anogenital carcinomas the degree of Sk‐v lysis was decreased in proportion to the duration of lesions (correlation coefficient–r = ‐0.79). Neither anti‐K‐562 nor anti‐Sk‐v cytotoxicities were significantly affected in patients with condylomata and with HPV‐6 associated verrucous carcinomas. Short‐term (3 hr) pre‐incubation of normal PBMC with sera from patients with BP and HPV‐16‐associated anogenital carcinomas resulted in significant inhibition of their ability to lyse Sk‐v cells. Lysis of K‐562 cells remained unaffected. In patients with carcinomas, the suppressive effect of sera was associated with a lowering of the ability of their PBMC to lyse Sk‐v cells (r = ‐0.79). In patients with longer tumor persistence, the suppressive effect of serum was proportionally higher (r = 0.86).
An anti‐CD5 immunotoxin for chronic lymphocytic leukemia: Enhancement of cytotoxicity with human serum albumin‐monensin
Authors: Hertler Andrew A. , Schlossman David M. , Borowitz Michael J. , Blythman Hildur E. , Casellas Pierre , Frankel Arthur E. 
Published: February 2007

AbstractFive patients with B‐cell chronic lymphocytic leukemia (B‐CLL) were treated with 6 courses of the anti‐CD5 immunotoxin T101‐ricin A chain (T101‐RTA). Each course consisted of 8 bi‐weekly infusions of T101‐RTA (7 or 14 mg/m2). The immunotoxin was well tolerated in all cases with no major toxicities. Though saturation of circulating leukemic cell‐associated target antigen was demonstrated by FACS analysis in all patients, no intact immunotoxin was detected in bonemarrow or lymph‐node aspirates. Pharmacokinetic studies revealed rapid clearance of T101‐RTA, with a half‐life of 43 min. None of the patients developed detectable titers of antibody against either T101 murine antibody or ricin A chain. Clinical response was limited to a rapid and transient fall in WBC count lasting less than 24 hr, most likely secondary to the antibody portion of the conjugate. In vitro, fresh B‐CLL cells were resistant to T101‐RTA at concentrations up to 10−8M, while fresh malignant T‐cells with a 10‐fold increase in expression of CD5 antigen were sensitive. In the presence of the enhancing agent human serum albumin‐monensin, fresh B‐CLL cells were sensitive to T101‐RTA, with an ID50 more than 2 logs below the maximal concentration of immunotoxin achieved in vivo. We conclude that T101‐RTA is a potentially useful agent in the treatment of T‐cell leukemias. In the presence of HSA‐monensin, this spectrum of activity may be extended to B‐CLL.
Screening for breast cancer in nijmegen. report of 6 screening rounds, 1975–1986
Authors: Peeters P. H. M. , Verbeek A. L. M. , Hendriks J. H. C. L. , Van Bon M. J. H. 
Published: February 2007

AbstractA population‐based screening programme for breast cancer was initiated in Nijmegen in 1975 with mammography as the only screening procedure. Up to January 1987, 6 screening rounds were carried out with a 2‐year screening interval. Rates of attendance, referral, biopsy and detection were calculated and numbers of interval cancers are presented in order to give a clear view of what repeated screening can accomplish in a population. At the first screening round the attendance rate was 87% for women under age 50 and 83% for women aged 50–64. For women aged 65 or over the initial attendance rate was 40%. Rates of attendance declined in subsequent years. Detection rates were highest for elderly women at their first examination: 9.5 per 1,000 screened women. Corresponding rates were 5.6 and 2.3 per 1,000 for women aged 50–64 and below 50 respectively. The positive predictive value for referral was, on average, 20% for women under age 50 and 50% for elderly women, although a sharp increase was seen in the last 2 screening examinations for all age‐groups. Predictive values for biopsy were higher: 30% on average for women aged under 50 and 60%–70% for elderly women, again with a sharp increase in the last 2 screening rounds. Interval cancer rates, calculated as the number of cancers occurring within 2 years among negatively screened women at risk, showed no particular trend and varied between 0.9–1.3 per 1,000 woman‐years after each screening round. Compared to screen‐detected cancers, interval cancers occurred more frequently in younger women. In women under age 50, the ratio between screen‐detected and interval cancer was about 1:1, while it was about 2:1 for elderly women.
Sequential therapy with recombinant interferons gamma and alpha in patients with unfavorable prognosis of chronic myelocytic leukemia: Clinical responsiveness to recombinant ifn‐α correlates with the degree of receptor down‐regulation
Authors: Bartsch H. H. , Pfizenmaier K. , Hanusch A. , Scheurich P. , Ücer U. , Nagel G. A. 
Published: February 2007

AbstractNatural and recombinant interferons (IFNs) have already demonstrated therapeutic efficacy, including cytogenetic remissions, in patients with chronic myelocytic leukemia (CML). We investigated at the level of ligand‐receptor interaction the question whether heterogeneity of receptor number or affinity might contribute to primary or secondary treatment failures in CML. We therefore analyzed IFN‐γ and IFN‐α receptor expression and regulation during treatment with recombinant IFN‐γ and IFN‐α in 15 patients with advanced CML. We found no difference in number or affinity of constitutively expressed IFN‐γ receptors (mean 1,100) and, on average, a 30% reduction of IFN‐α receptors (mean 750) on peripheral blood mononuclear cells (PBMNC) of patients with chronic or accelerated CML as compared to mature granulocytes and/or bone marrow cells of healthy controls, which express on average 1,050 and 1,100 IFN‐γ and IFN‐α receptors, respectively. While IFN‐γ receptor expression on PBMNC is not influenced upon treatment with rIFN‐γ, there is a substantial downregulation of IFN‐α receptors in the course of rIFN‐α therapy. Our data also show a differential pattern of receptor down‐regulation between patients achieving complete hematologic remission (CHR) (4 out of 12) compared with patients with partial hematologic remission (PHR) and non‐responders. We conclude that differences in IFN receptor number cannot explain primary or secondary treatment failures. However, the differential ligand induced downregulation of IFN‐α receptors in patients achieving CHR compared to those with PHR or non‐responders suggest a prospective value of IFN‐α receptor determination.
Combined oral contraceptives and liver cancer
Authors: Molina Ramiro , Martinez Luis , Salas Oriana , Dabancens Aifredo , Yun Tao , Zhi‐Heng Chen , Yong‐Wei Hu , Cuadros Alvaro , Aristizabal Nubia , Modan Baruch , Ron Elaine , Alfandary Esther , Mati J. G. , Kenya Patrick , Kungu Alfred , Gatei D. , Ibeziako Patrick A. , Abioye A. A. , Junaid T. A. , Aghadiuno Patrick U. , Apelo Ruben A. , De LaCruz Julietta R. , Baens Jose , Canlas Benjamin D. , Silpisornkosol Suporn , Pardthaisong Tieng , Sivasomboom Boonlong , Theetranont Choti , Boosiri Banpot , Chutivongse Supawat , Virutamasen Pramuan , Wongsrichanalai Chansuda , Sindhvananda Sermsri , Koetsawang Suporn , Rachawat Duangdao , Kiriwat Orawan , Chantarakul Nivat , Anthony P. P. , Thomas David B. , Stanford Janet L. , Ray Roberta M. , Noonan Elizabeth A. , Holck Susan 
Published: February 2007

AbstractA multi‐national, hospital‐based, case‐control study was conducted to evaluate the possible relationships of steroid contraceptives to 6 neoplasms. Based on data from 122 newly diagnosed cases of primary liver cancer and 802 matched controls, the relative risk of liver cancer in women who had ever used combined oral contraceptives was estimated to be 0.71 (95% Cl 0.4–1.2). No consistent trend in risk with months of use or time since first or last use was observed. Separate analyses also revealed no association between use of combined oral contraceptives and hepatocellular carcinoma (RR = 0.60) or cholangiocarcinoma (RR = 1.22). Most women in this study came from areas in which hepatitis B is endemic and rates of liver cancer are relatively high, and in most cases use of oral contraceptives was of short duration. These results provide no evidence that short‐term use of oral contraceptives enhances risk of liver cancer in countries where the determinants of this disease are similar to those observed in the countries where this study was conducted.
Inter‐laboratory variation as an explanation for varying prevalence estimates of human papillomavirus infection
Authors: Brandsma Janet , Burk Robert D. , Lancaster Wayne D. , Pfister Herbert , Schiffman Mark H. 
Published: February 2007

AbstractHuman papillomavirus (HPV) infection has been strongly implicated as a cause of genital neoplasia. Although Southern blot DNA hybridization techniques are regarded as the most accurate means of identifying HPV infection, studies using this technique to measure infection have provided varying estimates of the prevalence of HPV among healthy and diseased groups. This investigation provides a possible explanation for study‐to‐study differences, by demonstrating interlaboratory variability in the detection and typing of HPV by Southern blot. Four experienced laboratories tested 40 identical, masked samples of DNA extracted from presumed infected and non‐infected tissues. The pairwise percentage agreement between the laboratories in judging samples as HPV‐positive or negative ranged from 66 to 97%. Among samples judged to contain HPV, agreement as to type ranged from 77 to 96%. We conclude that inter‐laboratory differences are an important consideration in any discussion of HPV prevalence estimates.
Lectin‐resistant variants and revertants of mouse melanoma cells: Differential expression of a fucosylated cell‐surface antigen and altered metastasizing capacity
Authors: Finne Jukka , Castori Serenella , Feizi Ten , Burger Max M. 
Published: February 2007

AbstractIn order to evaluate the possible role of cell‐surface carbohydrates in metastasis of tumour cells, 2 wheat‐germ agglutinin‐resistant (WGAr) variants of B16 mouse melanoma and 8 back revertants selected with other lectins were analyzed with respect to the surface expression of fucosylated carbohydrate antigens and their metastasizing capacity. The variant cells, expressing a greatly increased fucosyltransferase activity, were found to express the fucose‐containing SSEA‐I antigen on their cell surface. The revertant cells selected for lower fucosylation with Lotus tetragonolobus lectin and ricin had lost this particular antigen. Seven of the 8 revertant lines also reverted back to a state of increased metastasizing capacity as compared to the WGAr variants they were derived from. A single one of the revertants displayed reduced metastasizing capacity, suggesting that additional changes can also be present in some of the cell lines. These results suggest a possible linkage between expression of the developmental SSEA‐I antigen and reduced metastasizing capacity in the mouse melanoma model.
Differential expression of n‐myc and c‐src proto‐oncogenes during neuronal and schwannian differentiation of human neuroblastoma cells
Authors: Horii Yoshihiro , Sugimoto Tohru , Sawada Tadashi , Imanishi Jiro , Tsuboi Kazuhiko , Hatanaka Masakazu 
Published: February 2007

AbstractNeuroblastoma (NB) cells can be induced to differentiate bidirectionally into either neuronal or schwannian cells by different inducers. However, the underlying mechanisms are poorly understood. We examined the expression of N‐myc and c‐src genes in 3 human NB‐cell lines during either neuronal or schwannian differentiation in vitro, since proto‐oncogenes are considered to play a pivotal role in regulating cell proliferation and differentiation. Decreased N‐myc expression and increased c‐src expression were observed during neuronal differentiation by retinoic acid, polyprenoic acid (E5166) and dibutyryl cyclic AMP, whereas the expression of N‐myc and c‐src genes was considerably reduced during schwannian differentiation by bromodeoxyuridine, demonstrating that the expression of N‐myc and c‐src genes was regulated independently in the bipolar differentiation processes of NB cells. Our results suggest that enhanced N‐myc expression might be closely linked to the undifferentiated phenotypes of NB cells, that c‐src expression might be related to the neuronal differentiation of NB cells, and that N‐myc and c‐src genes might be regulated independently in the determination of the bipolar differentiation of NB cells.
Recruitment and activation of tumor‐specific immune t cells in situ: Functional studies using a sponge matrix model
Authors: Zangemeister Uwe , Thiede Kerstin , Schirrmacher Volker 
Published: February 2007

AbstractThe activation of tumor‐specific precursor cytotoxic T lymphocytes (CTLP) into cytotoxic T cells (CTL) was demonstrated in situ using the well‐defined, highly metastatic ESb tumor as murine model system. Ten days after optimal immunization of syngeneic mice with a sublethal dose of live ESb tumor cells in the pinna, tumor‐sensitized non‐cytotoxic CTLP were recovered from the spleen and lymph nodes. These cells mature into tumor‐specific CTL upon restimulation in vitro. Using a confined sponge matrix compartment, in combination with a specific tumor vaccine (autologous inactivated tumor cells), we induced a CD8+ (Lyt 2+) T‐cell‐mediated, highly cytotoxic anti‐tumor immune response in situ in immunized mice. It was not possible to activate a similar response directly in lymphoid organs such as the spleen. The cytotoxic CD8+ T cells, recovered by simple mechanical pressing of the sponge, were active against the specific tumor cells in a 51Cr‐release assay in vitro and also in a Winn neutralization assay in vivo. CTL activity was increased and remained in the non‐adherent fraction when the cell mixture, squeezed out of the sponges, was passed over nylon wool. In a cell recruitment assay, the delayed‐type hypersensitivity (DTH) potential of the activated sponge‐infiltrating T cells was demonstrated by their capacity to recruit circulating host lymphocytes to sites of tumor‐cell location in situ.
Characterization of htlv‐i isolates and t lymphoid cell lines derived from french west indian patients with tropical spastic paraparesis
Authors: Gessain A. , Saal F. , Morozov V. , Lasneret J. , Vilette D. , Gout O. , Emanoil‐Ravier R. , Sigaux F. , De Thé G. , Périès J. 
Published: February 2007

AbstractLymphoid cell lines derived from the peripheral blood of French West Indian patients with HTLV‐I sero‐positive Tropical Spastic Paraparesis and HTLV‐I isolates were characterized. While patients' peripheral blood lymphocytes did not express detectable HTLV‐I antigens when uncultured, they did so after short‐term culture. Established cell lines were of T‐cell lineage: CD2+, CD3+, CD4+, CD7+, WT31+ with activated T‐cell markers CD25+ DR+ and a clonal rearrangement of the ß and γ genes of the T‐cell receptor. HTLV‐I antigens were detected in cell lines by indirect immunofluorescence, Western blot and radio‐immunoprecipitation assays. After 4 months in culture, low levels of Mg2+ dependent reverse transcriptase activity were detected and electron microscopy revealed numerous type‐C retroviral particles similar to HTLV‐I virions. Western blot and radio‐immunoprecipitation analysis of purified viruses revealed gp46, p24, p19 and Pr53gag proteins similar to those detected in HUT 102 and MT2 cell lines. Deep analysis of env‐coded precursor of one TSP versus ATL isolates revealed minor differences in their molecular weights. Southern blot analysis using 32P HTLV‐I env gene as a probe showed the presence of HTLV‐I proviral fragments clonally integrated into the genome of the cell lines. Our data suggest that HTLV‐I isolated from Tropical Spastic Paraparesis does not differ significantly from the leukemogenic prototypes. Does HTLV‐I induce either acute lymphoproliferative diseases or chronic neuromyelopathies depending upon as yet unknown co‐factors? This question remains to be determined.