bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deficient production of tumor necrosis factor by peripheral‐blood monocytes in chronic lymphocytic leukemia
Authors: Flieger Dimitri , Emmerich Berthold , Meyer Nicolette , Riethmüller Gert , Ziegler‐Heitbrock H. W. Löms 
Published: February 2007

AbstractThe production of tumor necrosis factor (TNF) by lipopolysaccharide (LPS)‐triggered peripheral‐blood mononuclear cells (PBM) was investigated in 23 patients with untreated B‐cell chronic lymphocytic leukemia (B‐CLL) and 14 control donors. Cells were stimulated at concentrations that reflect cell density in peripheral blood. Under these conditions, PBM from 11/23 of the CLL patients produced at least 10‐fold less TNF as compared with controls. Monocyte numbers were decreased in percentage, while absolute numbers (normal range 233 ± 120 × 103/mm3) were decreased only in 2, normal in 17 and increased in 4 patients indicating that the deficiency is not a result of monocytopenia in most patients. Cell separation experiments indicate that after removal of leukemic B cells, percentages of monocytes return to control range and TNF production is improved (7/7). In mixing experiments, we found a suppression of TNF production in control mononuclear cells by CLL cell samples (75 × 106 cells/ml) in 5/19 cases, while control cells from thymus exhibited no or little suppression in these conditions. In 2‐chamber experiments, leukemic samples suppress TNF production by normal monocytes across a 0.45μm membrane indicating that a soluble factor is responsible for suppression. The factor exhibits higher stability in serum‐free conditions and its molecular weight is below 20kDa. Prostaglandins are not involved, since indomethacin did not abrogate suppression.
Dietary factors and risk of lung cancer: Results from a case‐control study, toronto, 1981–1985
Authors: Jain M. , Burch J. D. , Howe G. R. , Risch H. A. , Miller A. B. 
Published: February 2007

AbstractAssociations between dietary factors and risk of lung cancer are reported from a study of 839 cases and 772 population‐based controls interviewed in metropolitan Toronto between 1981 and 1985. Increased consumption of vegetables is associated with a decreased relative risk of 0.60 (95% confidence limits =0.40 to 0.88) for those in the highest compared with the lowest quartile. Cholesterol intake is associated with increased risk, but this is restricted to those in the highest quartile for whom the relative risk is 1.58 (95% confidence limits =1.05 to 2.38) compared with those in the lowest quartile. The results of this study suggest that dietary factors may affect the risk of lung cancer, but identification of the specific constituents involved will require further research.
Protein kinase C‐mediated regulation of the expression of CD14 and CD11 /CD18 in U937 cells
Authors: Pedrinaci Susana , Ruiz‐Cabello Francisco , Gomez Ovidio , Collado Antonia , Garrido Federico 
Published: February 2007

AbstractWe used the U937 cell line to analyze CD14, CD11/CD18, HLA class‐I and DR antigen expression during PMA‐induced differentiation. Treatment of U937 cells with PMA markedly increased CD14, CDIIa, CDIIb and CDI8 antigen expression, and slightly increased CDIIc expression. Protein kinase C may play a major role in regulating the expression of these antigens. The protein kinase inhibitor H7 abrogated the inductive effect of PMA. Calcium ionophore, when added alone or in the presence of PMA, had no effect. The inhibitory effect of the calcium antagonist verapamil, EGTA, and of chlorpromazine, an antagonist of calcium‐binding proteins, supports a role for calcium‐dependent protein kinase C in the up‐regulation of CDI4 and CDII/CD18 surface expression. The specific calmodulin inhibitors R2457I and W7 had no effect on antigen expression. Our findings suggest that protein kinase C activation is an important step in the PMA‐induced differentiation of U937 cells.
Rat monoclonal antibodies to the external domain of the product of the C‐erbB‐2 proto‐oncogene
Authors: Styles Jennifer M. , Harrison Shona , Gusterson B. A. , Dean C. J. 
Published: February 2007

AbstractWith the breast carcinoma cell line BT 474 used as a source of antigen, four rat monoclonal antibodies (MAbs) (3 lgG2a and I IgA) have been prepared against the external domain of the product of the c‐erbB‐2 proto‐oncogene. All 4 antibodies stain frozen sections of tissues that over‐express the product of the c‐erbB‐2 proto‐oncogene, and competitive binding assays showed that the antibodies recognized 2 non‐overlapping epitopes. Representative antibodies from the two groups (ICR12 and 13) were shown to specifically immunoprecipitate a 190kDa protein from 35S‐methionine‐labelled breast carcinoma cells where the c‐erbB‐2 is amplified (BT 474 and MDA‐MB 361). Two of the antibodies (ICR12 and ICR17) bind to the denatured antigen in Western blots and ICR 12 stains formolsaline‐fixed sections of breast carcinoma tissue that overexpresses the product of the c‐erbB‐2 proto‐oncogene. These antibodies should be useful not only for immunocytochemical diagnoses but also for radio‐immunoscintigraphic and therapeutic applications.
Characterization of the synergistic effect of insulin and transferrin and the regulation of their receptors on a human colon carcinoma cell line
Authors: Watkins Linda F. , Lewis Laurie R. , Levine Alan E. 
Published: February 2007

AbstractThe human colon carcinoma cell line, HCT 116, can be grown in chemically defined media in the absence of exogenous growth factors. The addition of transferrin and insulin will significantly stimulate growth. The interaction of these growth factors with their receptors was studied to determine whether the synergistic action of insulin and transferrin on growth involved alterations in the growth‐factor receptors. Redistribution of the transferrin receptor occurred in the presence of transferrin or transferrin plus insulin. The presence of insulin in the growth media resulted in occupation of cell‐surface insulin receptors without a reduction in total insulin binding. Addition of transferrin with insulin resulted in a decrease in insulin binding to its receptor, with no alteration in receptor affinity. It appears that transferrin plays a role in regulating the insulin receptor and that this may contribute to the synergistic effect of insulin and transferrin on growth.
Cancer incidence among swedish brewery workers
Authors: Carstensen John M. , Bygren Lars Olov , Hatschek Thomas 
Published: February 2007

AbstractIn order to examine the risks of cancer, particularly of rectal cancer, among Swedish brewery workers, 6,230 men employed in the brewery industry in 1960 were followed‐up during 1961–79 by the Swedish Cancer Registry. Using all Swedish men as a reference group, relative risks (RR) were computed with standardization for year of birth, year of follow‐up, and geographic region. A total of 712 new cases of cancer were observed compared to 570.7 expected (p < 0.001). Significantly increased risks were seen for several cancer sites, e.g. esophagus (RR = 2.5, 95% confidence interval (Cl) = 1.5–3.8), rectum (RR = 1.7, Cl = 1.3–2.3), pancreas (RR= 1.7, C1=1.2‐2.3), and lung (RR = 1.4, Cl = 1.1–1.7). An excess risk of liver cancer was almost significant (p = 0.053, RR = 1.7, Cl = 1.0–2.8). The risk of colon cancer was not significantly increased (RR = 1.2, Cl = 0.9–1.5), and the difference between the relative risk of colon cancer and that of rectum cancer was nearly significant (p = 0.07). Our results support the hypothesis that high beer consumption is associated with an increased risk of rectal cancer.
Cytosolic NAD(P)H:(Quinone‐acceptor)oxidoreductase in human normal and tumor tissue: Effects of cigarette smoking and alcohol
Authors: Schlager John J. , Powis Garth 
Published: February 2007

AbstractNAD(P)H:(quinone‐acceptor)oxidoreductase (QAO), previously known as DT‐diaphorase, catalyzes the reduction of qui‐nones to hydroquinones. Enhanced activity of the enzyme has been suggested to protect cells against the cellular toxicity and carcinogenicity of quinones, but may activate some cyto‐toxic anti‐tumor quinones. Cytosolic levels of QAO, carbonyl reductase (CR) and total quinone reductase activity have been measured in normal and tumorous human tissues. QAO was the major component of the total cytosolic quinone reductase activity in all the tissues investigated. CR represented 10 to 28% of the total cytosolic quinone reductase activity in normal tissue. Normal tissue QAO was high in the stomach and kidney, and lower in the lung, liver, colon and breast. Primary tumor from lung, liver, colon and breast had elevated levels of QAO compared to normal tissue, while tumor from kidney and stomach had lower levels. CR was not significantly altered in tumor tissue, except in the case of lung and colon tumor which showed an increase compared to normal tissue. A major determinant of the variability of human lung tumor QAO was the cigarette‐smoking history of the donor. Non‐smokers and past smokers had high levels of tumor QAO compared to normal tissue. Smokers had levels of tumor QAO that were not significantly different from those of normal tissue QAO. Smokers had a small increase in normal lung QAO compared to non‐smokers. Alcohol use was associated with an increase in lung tumor QAO but had no effect on QAO in normal lung. The function of QAO in tumors is not known but the elevated activity of QAO in some tumors and the apparent depressant effect of smoking could influence the response of these tumors to quinone drugs or toxic agents that are metabolized by QAO.
Cervical‐cancer screening: Attendance and cost‐effectiveness
Authors: Koopmanschap Marc A. , van Oortmarssen Gerrit J. , van Agt Heleen M. A. , van Ballegooijen Marjolein , Habbema J. Dik F. , Lubbe Koos Th. N. 
Published: February 2007

AbstractThe influence of attendance on the health effects and cost‐effectiveness of cervical‐cancer screening was studied, both for organized screening programmes and for spontaneous screening. The asymmetric distribution of smears among the female population and the higher risk incurred by women who never or only occasionally attend screening appear crucial in determining the health effects of screening. An increase in attendance rate induces a substantial rise in health effects and a less than proportionate rise in costs, thus improving cost‐effectiveness. Wider screening coverage, in order to increase the number of life‐years saved, is achieved more efficiently by encouraging a greater number of women to attend than by inviting the same number of women to attend more often, i.e., with a shorter interval between successive screens. Spontaneous screening is characterized by high coverage for younger women and low coverage for middle‐aged and older women. This leads to a small amount of health effects and poor cost‐effectiveness as compared with organized screening.
Hodgkin's disease in adults: Association with social factors and age at tonsillectomy. A case‐control study
Authors: Bonelli Luigina , Vitale Vito , Bistolfi Franco , Landucci Marco , Bruzzi Paolo 
Published: February 2007

AbstractThe relationship between socio‐economic characteristics, previous tonsillectomy, family history of cancer and risk of Hodgkin's disease (HD) was investigated in a case‐control study. One hundred and sixty patients, aged 15–78, with histologically confirmed HD, and 185 hospital controls were interviewed. A statistically significant decrease in risk of HD was observed among subjects with large sibship size (RR = 0.63, C.I. 0.46–0.86) and among those who underwent tonsillectomy prior to the age of 10 (RR = 0.46, C.I. 0.22–0.94). High educational level was associated with an increased risk of HD (RR = 6.68, C.I. 1.94–23.08). Analysis by age‐group (15–39 yrs and 40 yrs or more) confirmed the role of high educational level as a risk factor in both young and old subjects, while the protective effect of tonsillectomy at an early age seems to be confined to young subjects. Analysis by histological subtype showed a statistically significant decrease in risk of HD in large sibship size for both nodular sclerosis (NS) and mixed‐cell subtypes; the role of education and tonsillectomy was observed only for NS. These data confirm the role of social factors in HD risk and suggest a protective effect of tonsillectomy performed early in life that needs to be confirmed in larger population‐based studies.
Expression of the p185 encoded by her2 oncogene in normal and transformed human tissues
Authors: Natali Pier G. , Nicotra Maria R. , Bigotti Aldo , Venturo Irene , Slamon Dennis J. , Fendly Brian M. , Ullrich Axel 
Published: February 2007

AbstractThe human homolog of the rat neu oncogene, HER2 (also termed c‐erbB2) has been demonstrated in amplified form in human breast tumors with poor prognosis. Although amplification of the gene correlates with expression of a 185‐kDa transmembrane glycoprotein, no extensive information is available regarding the extent of tissue and tumor specificity of this gene product. We have addressed this issue by immunohistochemically evaluating the expression of p185 HER2 in normal tissue and various tumors using monoclonal antibodies (MAbs) to distinct epitopes of its extracellular domain. No detectable levels of p185 HER2 were found in fetal tissues analyzed, with the exception of renal tubules in 2 out of 3 specimens tested and in intestinal epithelium. In adult tissues, detectable levels of this glycoprotein were found in a restricted number of cell types, the expression being heterogeneous among individuals and cell histotypes. Among the neoplasms assayed p185 HER2 was expressed in 46% of primary breast cancers, in 28% of ovarian tumors and in 30% of colon rectum malignancies. No male breast adenocarcinomas were p185‐positive. A large number of other tumors tested revealed only a low incidence of expression of the p185. In metastatic breast tumors p185 HER2 was demonstrated homogeneously among multiple autologous lesions and almost invariably (80%) the expression of p185 in the primary lesion correlated with that of the deriving metastases. Our findings indicate that the expression of the p185 HER2 represents a tumor marker of clinical relevance in breast cancer. Whether this holds true for other malignancies remains to be explored.
Heavy water enhances the antineoplastic effect of 5‐fluoro‐uracil and bleomycin in nude mice bearing human carcinoma
Authors: Altermatt Hans Jörg , Gebbers Jan‐Olaf , Laissue Jean Albert 
Published: February 2007

AbstractThe antineoplastic effect of heavy water on the growth of xenotransplanted human carcinoma was compared to that of 2 cytostatic drugs. Seven‐week‐old BALB/c‐nu/nu mice were inoculated subcutaneously with a poorly differentiated oropharyngeal squamous‐cell carcinoma, or with variants of carcinoma of the large intestine. After tumor inoculation, 3 subgroups of mice were treated by: (i) moderately deuterated drinking water; (ii) i.p. injections of 5‐Fluoro‐uracil (5‐FU) or Bleomycin; (iii) a combination of deuterated drinking water and of the cytostatic drugs. Control mice were not treated. Heavy water delayed growth of all carcinoma variants. The cytostatic drugs slowed down the growth of the 2 poorly differentiated tumor variants. Conversely, 5‐FU did not retard the growth of the moderately well differentiated colon carcinoma. Heavy water combined with either cytostatic drug showed synergistic effects in the 2 poorly differentiated tumor variants. The tumors of treated animals weighed 36% to 90% less than those of control animals. The antineoplastic effects were more conspicuous in poorly than in moderately well differentiated tumor variants. Cytokinetic parameters such as labelling indices following application of 5‐125I‐Iodo‐2′deoxyuridine, and of Ki‐67, a monoclonal antibody (MAb) directed against an antigen of proliferating cells, or mitotic indices and tumor volume doubling time, combined with the results of histologic evaluation of the tumors, suggested an underlying deuterium‐induced prolongation of tumor‐cell cycle times and a reduction of the growth fraction.
Disseminated or localized growth of a human B‐cell tumor (Daudi) in scid mice
Authors: Ghetie Maria‐Ana , Richardson James , Tucker Thomas , Jones Diane , Uhr Jonathan W. , Vitetta Ellen S. 
Published: February 2007

AbstractA human Burkitt lymphoma (Daudi) has been grown in the mutant mouse called C.B‐17 SCID. Twenty‐eight days after s.c. injection of Daudi cells, a palpable tumor grew only at the site of injection in all injected mice. In contrast, after intravenous (i.v.) or intraperitoneal (i.p.) injection, macroscopic, disseminated tumors developed. Following i.v. inoculation, tumors grew in the lungs, kidneys, ovaries and adipose tissue, and microscopic tumor infiltrates were observed in the spleen, bone marrow, spinal column and femur, whereas after i.p. injection, the tumors were localized in the abdomen, liver, spieen, ovaries and muscular tunics of the gut, but did not disseminate into the lung or bone marrow. The growth pattern and phenotype of the Daudi cells were similar whether the inoculated tumor cells were derived from the in vitro cell line or from in vivo passaged tumors. The survival time of the tumor‐bearing animals was dependent on the dose of i.v.‐administered Daudi cells; as few as 100 cells caused death. All mice injected i.v. showed paresis or paralysis of the hind legs just prior to death. This was associated with the presence of neoplastic nodules within the spinal canal. Two surface antigens on Daudi cells (CD19 and CD22) were stably expressed in all the neoplastic lesions. Radiolabelled anti‐CD22 antibodies localized in organs infiltrated with tumor, but did not penetrate primary s.c. tumors.This model of disseminated vs. solid tumor should prove useful for evaluating the efficacy of different types and doses of therapeutic antibodies, immunoconjugates and immunotoxins prepared from anti‐human B‐cell antibodies.
Aspergillus species strain m39 produces two naphtho‐γ‐pyrones that reverse drug resistance in human KB cells
Authors: Ikeda Shun‐Ichi , Sugita Masanori , Yoshimura Akihiko , Sumizawa Tomoyuki , Douzono Haruhiko , Nagata Yukihiro , Akiyama Shin‐Ichi 
Published: February 2007

AbstractOne thousand fungi and Actinomycetes were investigated to see whether they produced compounds that reverse multidrug resistance in KB cells. Only one Aspergillus strain M39 produced agents with resistance‐reversing activity and these compounds were identified to be rubrofusarin B and dianhydro‐aurasperone C. Rubrofusarin B only slightly reversed the resistance of KB‐C2 cells to Adriamycin and daunomycin, partially reversed the resistance to chromomycin A3, and almost completely reversed the resistance to vincristine and mitomycin C. Purified dianhydro‐aurasperone C and rubrofusarin B had similar effects on drug resistance in KB‐8‐5 cells. Dianhydro‐aurasperone C enhanced the accumulation of vinblastine in KB‐8‐5 cells and inhibited the efflux of vinblastine from the cells. Dianhydro‐aurasperone C and rubrofusarin B at 10 μ M completely inhibited 3H‐azidopine photolabelling of P‐glycoprotein. The two products of Aspergillus strain M39 appear to reverse multi‐drug resistance by interacting with P‐glycoprotein and inhibiting its role as an active efflux pump.
Differences in C‐myc and pvt‐1 amplification in sewa sarcoma sublines selected for adherent or non‐adherent growth
Authors: Minarovits Janos , Steinitz Michael , Boldog Ferenc , Imreh Stephan , Wirschubsky Zvi , Ingvarsson Sigurdur , Hedenskog Mona , Minarovits‐Kormuta Susanna , Klein George 
Published: February 2007

AbstractConversion of solid sarcomas and carcinomas into ascites tumors depends on the in vivo selection of phenotypically altered tumor cell variants that can grow in the dissociated form. Once selected, they retain this property even after prolonged s.c. growth as solid tumors. From an s.c.‐passaged subline of an ascites‐converted murine sarcoma (SEWA‐AS12), we were able to separate cells adapted to the ascites form of growth from cells that can only grow in the solid form on the basis of their differential adherence to plastic. Both c‐myc and pvt‐1 were amplified approximately 63‐ to 77‐fold in the nonadherent subline (SEWA‐AS12‐NA), but only 5‐ to 8‐fold in the adherent subine (SEWA‐AS12‐ADH). This suggests that c‐myc and/or pvt‐1 amplification may provide a selective advantage to cells that can grow in the dissociated form.
Del cell line: A “malignant histiocytosis” CD30 + T(5;6)(Q35;P21) cell line
Authors: Barbey Sylvette , Gogusev Jean , Mouly Héléne , Le Pelletier Odile , Smith Wandy , Richard Stephane , Soulie Joëlle , Nezelof Christian 
Published: February 2007

AbstractA new cell line DEL, established in vitro, was isolated from a pleural effusion of a boy who died of malignant histiocytosis. Its principal characteristics are: strong positivity with monoclonal antibodies (MAbs) to CD25, CD30, CD45R, KiM7, EMA, HLA CI I and II; constant presence of acid phosphatase, ANAE, α‐anti‐trypsin, α‐anti‐chymotrypsin and NBT reduc‐tase activity; rearrangement of the immunoglobulin heavychain gene (JH) and a germ‐line configuration of the T‐chain gene; and finally a translocation between chromosomes 5–6 with a breakpoint in 5q35. The DEL cell line is appropriate for studying the role of the 5q localized c‐fms oncogene and of the genes of the mononuclear phagocyte growth factor (CSFI) and of their receptors in the dynamics and etiology of malignant hemopathies associated with a 5q35 breakpoint.
Further characterization, isolation and identification of the epithelial cell‐surface antigen defined by monoclonal antibody aua1
Authors: Durbin H. , Rodrigues N. , Bodmer W. F. 
Published: February 2007

AbstractThe human epithelial antigen recognized by monoclonal antibody (MAb) AUAl has been characterized as a cell‐surface glycoprotein. It has been isolated from human colonic mucosa by AUAl affinity separation. N‐terminal peptide sequence of this purified material has revealed a 17 amino acid sequence which identifies it with one of a group of similar epithelial/tumour‐associated glycoproteins defined by a variety of MAbs. Using the polymerase chain reaction to map the gene encoding this antigen, our previous AUAl antigen assignment to chromosome 2 has been confirmed.
Dietary factors and risk of pancreatic cancer: Results of a Canadian population‐based case‐control study
Authors: Howe G. R. , Jain M. , Miller A. B. 
Published: February 2007

AbstractDietary information from a case‐control study of pancreatic cancer conducted in Metropolitan Toronto between 1983 and 1986 is reported. A total of 249 cases and 505 populationbased controls completed quantitative diet histories from which total caloric intake and the intake of a number of nutrients were estimated. A positive association with total caloric intake was observed with a relative risk of 2.39, 95% confidence interval 1.18–4.83 (highest versus lowest quartile), due primarily to the intake of carbohydrates. Inverse associations were seen with fibre from fruit, vegetable and cereal sources, with a relative of risk of 0.42, 0.22–0.78 (highest versus lowest quartile), for total fibre intake.
Cancer in Jewish migrants to Israel
Authors: Parkin D. M. , Steinitz R. , Khlat M. , Kaldor J. , Katz L. , Young J. 
Published: February 2007

AbstractData on the incidence of 15 cancers in the Jewish population of Israel from the period 1961–1981 have been studied with emphasis on the risk of disease in relation to birthplace, calendar time, and duration of residence in Israel. The results show wide variations in incidence by birthplace, from less than 2‐fold for pancreatic cancer to a 40‐fold difference for oesophageal cancer in females; the reasons for these are often little understood. The potential importance of environmental agents in aetiology for different migrant groups is discussed in relation to the size and rapidity of changes in risk related to the duration of residence in Israel.