bluuebunny/crossref_metadata_2025_split huggingface dataset:
Polymorphism of 5′‐region of the bovine growth hormone receptor gene
Authors: Maj A. , Pareek C.S. , Klauzińska M. , Zwierzchowski L. 
Published: November 2005

SummaryGenes coding for growth hormone (GH) and GH receptor (GHR) are candidates for quantitative trait markers in farm animals. This work describes a search for nucleotide sequence polymorphisms within the 5′‐region of the bovine GHR gene. Two new single nucleotide polymorphisms were found: restriction fragment length polymorphisms (RFLPs) at a Fnu4HI/TseI site (C/T transition at position −1104), and at a Sau96I site (C/T transition at position −262). The Fnu4HI/TseI polymorphic site is located within the 1.2‐kbp LINE‐1 retrotransposon upstream of the P1 promoter, while the Sau96I RFLP locates in the P1 promoter for exon 1A. The appearance of the Sau96I RFLP was studied in representatives of two bovine species, Bos taurus and Bos indicus. An absolute correlation was observed between Sau96I genotype and the insertion/deletion of LINE‐1.
Effects of Helicobacter pylori infection on mucin expression in gastric carcinoma and pericancerous tissues
Authors: WANG RONG‐QUAN , FANG DIAN‐CHUN 
Published: November 2005

AbstractBackground:  Helicobacter pylori infection is one of the major causes of human gastric carcinoma and can disturb the gastric mucosa barrier. Mucins have not only lubricating and protecting functions, but are also related to signal transduction, turnover of gastric epithelium and carcinogenesis of gastric mucosa. The aim of this study was to investigate the relationship between H. pylori infection and aberrant mucin expression in patients with gastric carcinoma.Methods:  H. pylori infection was diagnosed by the Warthin–Starry staining method. Different kinds of mucins were detected using an immunohistochemical method.Results:   Of 46 patients with gastric carcinoma, there were 26 patients who had H. pylori infection (56.5%). Of 21 pericancerous mucosas from the H. pylori‐positive patients, 14 had MUC2 expression (66.7%), seven had strong MUC1 expression (+ + +) (33.7%), seven had strong MUC6 expression (+ + +) (33.3%), and five had strong MUC5AC expression (+ + +) (23.8%). In contrast, only six of 18 H. pylori‐negative pericancerous mucosas had MUC2 expression (33.3%) (P < 0.05 compared with H. pylori‐positive pericancerous mucosas), 12 had strong MUC1 expression (+ + +) in 16 H. pylori‐negative pericancerous mucosas (75%) (P < 0.05), 11 had strong MUC6 expression (+ + +) in 16 H. pylori‐negative pericancerous mucosas (68.8%) (P < 0.05), and 10 had strong MUC5AC expression (+ + +) in 14 H. pylori‐negative pericancerous mucosas (71.4%) (P < 0.01). Of the H. pylori‐positive cancerous tissues, 50% (13/26) had MUC1 expression and 38.5% (10/26) had MUC6 expression. In comparison, of the H. pylori‐negative cancerous tissues, 80% (16/20) had MUC1 expression (P < 0.05) and 80% (16/20) had MUC6 expression (P < 0.01).Conclusions:  The results indicate that H. pylori infection can alter the expression of some mucin genes in pericancerous mucosa and cancerous tissues of gastric carcinoma, then destroy the gastric mucosa barrier.
Ratio of circulating follistatin and activin A reflects the severity of acute liver injury and prognosis in patients with acute liver failure
Authors: LIN SHI DE , KAWAKAMI TADASHI , USHIO AKIRA , SATO AKIHIRO , SATO SIN‐ICHIRO , IWAI MASAKATSU , ENDO RYUJIN , TAKIKAWA YASUHIRO , SUZUKI KAZUYUKI 
Published: November 2005

AbstractBackground and Aim:  The activin A–follistatin system is known to play a critical role in hepatocyte regeneration during the repair of liver tissue. However, the relationship between blood levels of these compounds and the severity and prognosis of acute liver injury remains unclear. The aim of this study was to evaluate the clinical significance of circulating activin A and follistatin in patients with acute liver disease.Methods:  Serum activin A and plasma follistatin levels were determined on admission by enzyme‐linked immunosorbent assay in 32 patients with acute hepatitis (AH), 23 patients with acute severe hepatitis (ASH) and 16 patients with acute liver failure (ALF).Results:  Both serum activin A and plasma follistatin levels were significantly elevated in patients with ASH and ALF when compared with those in patients with AH and normal controls (NC). Although plasma follistatin levels were significantly and positively correlated with serum activin A levels (r = 0.413, P < 0.001), the follistatin and activin A (F/A) ratio showed distinct deviation from NC between AH and ALF patients. The F/A ratio in AH patients was significantly elevated when compared with NC, but was significantly reduced in ALF patients. Furthermore, the F/A ratio in non‐surviving ALF patients was significantly lower than that in survivors. Levels of serum activin A and plasma follistatin were significantly and negatively correlated with prothrombin time (PT) and normotest (NT) levels, while the F/A ratio showed significant and positive correlations with PT and NT.Conclusions:  Decreased blood F/A ratio in ALF patients may be a reliable indicator of the severity of acute liver injury and prognosis in ALF.
Is there ileopathy in portal hypertension?
Authors: RANA SURINDER S , BHASIN DEEPAK K , JAHAGIRDAR SUJEET , RAJA KAISER , NADA RITAMBHRA , KOCHHAR RAKESH , JOSHI KUSUM 
Published: November 2005

AbstractBackground and Aims:  Portal hypertensive gastropathy and colopathy are well described endoscopic abnormalities in patients with portal hypertension. Endoscopic abnormalities in the ileum in patients with portal hypertension have not been well described. The aim of the present study was to evaluate endoscopic abnormalities in the ileum of patients with portal hypertension.Methods:  Patients with portal hypertension of various etiologies were included in the study. Upper gastrointestinal endoscopy was performed to record esophageal varices, gastric varices and portal hypertensive gastropathy. Colonoscopy with retrograde intubation of the ileum was performed and the presence of colorectal varices, colopathy and mucosal findings in the ileum were noted.Results:  Forty‐one patients (age 16–80 years, 33 men) were studied. Esophageal varices were present in all. Portal hypertensive gastropathy was present in 27/41 (66%) patients. Rectal varices were noted in 22/41 (54%) patients and 17/41 (42%) patients had features suggestive of colopathy. Ileum could be intubated in 38 patients (93%). Endoscopic abnormalities in the ileum were noted in 13/38 (34%) patients. Ileopathy as evident by endoscopic mucosal abnormalities was observed in 10/38 (26%) patients. Ileal varices were present in 8/38 (21%) patients. Three of these had ileal varices alone while the remaining five patients also had associated ileopathy The presence of ileopathy was significantly associated with the presence of portal hypertensive gastropathy and colopathy but not with esophageal, gastric or rectal varices.Conclusions:  Ileopathy occurs in one‐third of patients with portal hypertension and is significantly associated with the presence of portal hypertensive gastropathy and colopathy.
Maintenance therapy with H2‐receptor antagonist until assessment of Helicobacter pylori eradication can reduce recurrence of peptic ulcer after successful eradication of the organism: prospective randomized controlled trial
Authors: MURAKAMI KAZUNARI , SATO RYUGO , OKIMOTO TADAYOSHI , WATANABE KOICHIRO , NASU MASARU , KODAMA MASAAKI , FUJIOKA TOSHIO 
Published: November 2005

AbstractPurpose:  This study examined the possible relationship between peptic ulcer recurrence and the presence or absence of maintenance therapy with an H2‐receptor antagonist performed until evaluation of Helicobacter pylori eradication.Methods:  The subjects were 483 patients with peptic ulcer (281 gastric ulcer and 202 duodenal ulcer) who were diagnosed as H. pylori positive. After receiving eradication therapy for H. pylori, patients were allocated at random to one of three different maintenance therapies: control group (no maintenance therapy), H2‐receptor antagonist half‐dose group, and H2‐receptor antagonist full‐dose group. The maintenance therapy was performed for 4 weeks until evaluation of H. pylori eradication.Results:  Among the 25 patients with a recurrent ulcer, 18 patients (72%) had a recurrence at the time of or before evaluation of H. pylori eradication. In the control group, the rate of ulcer recurrence occurring before evaluation of H. pylori eradication was 10.5% (14/133). This rate was significantly higher than those in the H2‐receptor antagonist half‐dose group (2.9%, 4/136) and the full‐dose group (0%, 0/135).Conclusion:  The results of this study suggest that maintenance therapy with an H2‐receptor antagonist performed after eradication therapy until evaluation of H. pylori eradication is likely to greatly reduce the ulcer recurrence rate without affecting evaluation of H. pylori eradication.
Efficacy of camostat mesilate compared with famotidine for treatment of functional dyspepsia: Is camostat mesilate effective?
Authors: ASHIZAWA NOBUO , HASHIMOTO TOMOYUKI , MIYAKE TATSUYA , SHIZUKU TOSHIHIRO , IMAOKA TOMONORI , KINOSHITA YOSHIKAZU 
Published: November 2005

AbstractBackground:  Differentiation between functional dyspepsia and early chronic pancreatitis is difficult because these diseases do not produce specific abnormalities in laboratory testing. The aim of this study was to examine the potential efficacy of camostat mesilate, a protease inhibitor, against functional dyspepsia and to characterize patients with favorable responses.Methods:  Dyspeptic patients who exhibited no abnormalities on laboratory blood and urine testing, abdominal ultrasonography and upper gastrointestinal endoscopy were randomized to receive camostat mesilate 200 mg three times daily or famotidine 20 mg twice daily for 4 weeks. Symptoms severity was recorded before and at 2 and 4 weeks after starting treatment using a visual analog scale.Results:  Epigastralgia was significantly improved after 2 and 4 weeks of treatment in both groups (P < 0.01); this improvement tended to be more marked in the camostat mesilate group (P < 0.05 at 2 weeks). The beneficial effect of camostat mesilate on epigastralgia was more prominent in chronic alcohol drinkers at 2 weeks (P < 0.05) and 4 weeks (P < 0.01).Conclusions:  Camostat mesilate is superior to famotidine for relieving epigastralgia in patients with functional dyspepsia. Its pain‐relieving effect is greater in patients who habitually drink alcohol.
How patients experience psoriasis: results from a European survey
Authors: Fouéré S , Adjadj L , Pawin H 
Published: November 2005

ABSTRACTBackground  Most of the psoriasis that affects the European population is treated with topical preparations. Compliance rates, however, are known to be quite low.Objective  To provide an update on how psoriasis is experienced by patients and how well these patients comply with prescribed topical treatment.Methods  This was an observational and transversal survey conducted in 1281 patients with psoriasis. Patient input alone formed the basis of the study. Participants were contacted through the national psoriasis patient associations in France, the United Kingdom, Belgium, Germany and the Netherlands.Results  The study showed that 32% of the patients suffer currently from psoriasis in the face, skin folds and genital areas – areas very sensitive to topical treatments. Most of the patients (74%) considered their psoriasis as at least moderately severe, and 73% stated not to comply with their current treatment. Lack of efficacy and messiness of the treatment were the main reasons for non‐compliance, an element that is especially important for the use in sensitive areas such as the face, skin folds and genitalia.Conclusion  The present survey confirmed that cosmetic acceptability is, together with efficacy and safety, a very important aspect of a successful treatment of psoriasis.
Rheological properties of three different vitamin D ointments and their clinical perception by patients with mild to moderate psoriasis
Authors: Marty JP , Lafforgue C , Grossiord JL , Soto P 
Published: November 2005

ABSTRACTBackground  Ointments, classically used for the treatment of dermatological diseases, are monophasic viscous semisolid formulations. According to the proportion of their compounds, they have physicochemical and organoleptic properties and when applied on skin show a specific behaviour allowing to be spread more or less easily.Objective  To measure in vitro rheological characteristics of three vitamin D derivative ointments prescribed for the treatment of psoriasis, and to compare their viscosity and clinical acceptability when applied on the diseased skin.Methods  Rheological characteristics of tacalcitol 4 µg/g, calcipotriol 50 µg/g and calcitriol 3 µg/g ointments were assessed by measuring the oscillatory viscoelastic parameters and the permanent flow analysis. Clinical acceptability was studied in 20 psoriatic male or female subjects, aged 18 years or older. A survey evaluated the acceptability of calcitriol vs. tacalcitol and calcipotriol. Questions included information about fluidity, spreading capacity and stickiness after application.Results  We demonstrated that viscoelastic parameters were four times higher for ointment tacalcitol than for calcipotriol and calcitriol, corresponding to a higher consistency of ointment tacalcitol compared to calcipotriol and calcitriol showing both similar results; better fluidity was demonstrated by calcitriol than by tacalcitol and calcipotriol. Comparable results were obtained for the quality to be spread. The sensation of stickiness, significantly different between tacalcitol and calcitriol, was not different between calcipotriol and calcitriol.Conclusion  The above results confirm the relationship between rheological in vitro and sensorial in vivo results: variations between different formulations may have an important influence on non‐adherence and treatment failure.
The right dose in the right place: an overview of current prescription, instruction and application modalities for topical psoriasis treatments
Authors: Savary J , Ortonne JP , Aractingi S 
Published: November 2005

ABSTRACTBackground:  Compared to treatment success with oral medications, treatment success with topical treatments is currently difficult to achieve.Aim:  To assess and compare existing methods of lesion size evaluation, prescription habits, instruction and application modalities of topical medications.Methods:  Review of current and new procedures to estimate the body surface area, to calculate the most adequate quantity of medication to be prescribed, to instruct patients and to assess the individual dosing procedures of topical treatments.Results:  The most accurate method to assess the body surface area remains the rule of nine, allowing, together with a recently developed calculation disc, to estimate the most adequate quantity of topical treatment (here calcitriol 3 g/g ointment) to be prescribed. Precise instructions to the patient are other important elements for the successful treatment of dermatoses. Dosing devices, such as a spatula, may help patients to dose their daily topical treatment, avoiding over‐ and underdosing of the medication.Conclusion:  A correct evaluation of the lesions sizes, a precise calculation of the treatments quantity to be prescribed, clear instructions and treatment‐specific individual dosing devices may help to achieve higher patient compliance and treatment success.
Therapy of paraneoplastic pemphigus with Rituximab: a case report and review of literature
Authors: Barnadas MA , Roe E , Brunet S , Garcia P , Bergua P , Pimentel L , Puig L , Francia A , García R , Gelpí C , Sierra J , Coll P , Alomar A 
Published: November 2005

AbstractParaneoplastic pemphigus (PNP) is an autoimmune blistering disease with poor prognosis when associated with malignant neoplasm. We report the case of a patient with PNP associated with a CD20+ non‐Hodgkin follicular lymphoma who was treated with Rituximab plus corticosteroids and short courses of cyclosporin. One and a half years after Rituximab therapy, oral ulcerations had cleared and oral methylprednisolone was slowly tapered down without further recurrences. In the course of the disease, the patient developed sepsis due to Listeria monocytogenes and viral infections by human herpes virus 1 and 3. At the end‐stage of the disease she developed a cutaneous infection from Mycobacterium chelonae. The patient died 2 years and 7 months after the onset of PNP. Rituximab may be useful for PNP therapy, but further studies are necessary to confirm this hypothesis.
Generalized granuloma annulare associated with chronic hepatitis B virus infection
Authors: Ma HJ , Zhu WY , Yue XZ 
Published: November 2005

AbstractWe describe a 63‐year‐old Chinese woman with generalized granuloma annulare (GGA) associated with chronic hepatitis B virus (HBV) infection for 10 years. She developed non‐annular papule lesions after a previous infection of hepatitis B virus. Since then, her clinical course was aggressive with involvement of the trunk, extremities and neck. Histologically, granulomatous inflammations were found in the upper to mid dermis. The HBV DNA in these lesions was demonstrated by polymerase chain reaction (PCR). GGA was totally regressed after treatment of interferon‐α for 3 months. To the best of our knowledge, GGA associated with chronic HBV infection has never been reported in literature. The prognostic significance of the association of granulomatous inflammation and virus infection is reviewed.
Caregivers as Managers of Subjective Wellbeing: A Homeostatic Perspective
Authors: Cummins Robert A. 
Published: November 2005

Background  This paper proposes that the adequacy of service delivery and caregiving to people who are disabled should be assessed using two criteria. One is the objective circumstance of living, which should be at a standard acceptable to the community at large. The other is a level of subjective wellbeing (SWB) within the normal range.Method  This latter criterion is a novel conception based on an understanding that SWB is homeostatically managed to lie normally within a narrow range of values.Results  People who have a disability are more likely than usual to have a fragile homeostatic system because of the additional life challenges imposed by their disability.Conclusions  The role of a caregiver is to provide resources and protection against strong threats to homeostasis, thereby ensuring that the person in their care has a normal‐range level of SWB.
The Environment in the International Classification of Functioning, Disability and Health
Authors: Chapireau Francois 
Published: November 2005

Background  The World Health Organization has adopted two classifications relating to disability, one was published in 1980 and the more recent one in 2001. Although the international classification of functioning, disability and health (ICF) was drafted as a revision of the international classification of impairments, disabilities and handicaps (ICIDH), the ICF is based on major changes when compared with the ICIDH. One of them has to do with the environment.Method  Quotations from the classification manuals and related articles are presented in order to make clear the scope of the environment in the ICF.Results  The ICF has a universal application. The gap between capacity and performance reflects the barriers created by the environment.Conclusions  In the ICF, universalism and barriers have specific meanings, reflecting specific policy choices.
Heritability of background EEG across the power spectrum
Authors: Smit D. J. A. , Posthuma D. , Boomsma D. I. , De Geus E. J. C. 
Published: November 2005

AbstractWe estimated the genetic and nongenetic (environmental) contributions to individual differences in the background EEG power spectrum in two age cohorts with mean ages of 26.2 and 49.4 years. Nineteen‐lead EEG was recorded with eyes closed from 142 monozygotic and 167 dizygotic twin pairs and their siblings, totaling 760 subjects. We obtained power spectra in 24 bins of 1 Hz ranging from 1.0 to 25.0 Hz. Generally, heritability was highest around the alpha peak frequency and lower in the theta and delta bands. In the beta band heritability gradually decreased with increasing frequency, especially in the temporal regions. Genetic correlations between power in the classical broad bands indicated that half to three‐quarters of the genetic variance can be attributed to a common source. We conclude that across the scalp and most of the frequency spectrum, individual differences in adult EEG are largely determined by genetic factors.
Lifespan extension in hypomorphic daf‐2 mutants of Caenorhabditis elegans is partially mediated by glutathione transferase CeGSTP2‐2
Authors: Ayyadevara Srinivas , Dandapat Abhijit , Singh Sharda P. , Beneš Helen , Zimniak Ludwika , Reis Robert J. Shmookler , Zimniak Piotr 
Published: November 2005

SummaryElectrophilic stress caused by lipid peroxidation products such as 4‐hydroxynonenal (4‐HNE) and/or related compounds may contribute to aging. The major mode of 4‐HNE metabolism involves glutathione conjugation catalyzed by specialized glutathione transferases. We have previously shown that glutathione transferase CeGSTP2‐2, the product of the Caenorhabditis elegans gst‐10 gene, has the ability to conjugate 4‐HNE, and that its overexpression extends lifespan of C. elegans. We now demonstrate that the expression level of CeGSTP2‐2 correlates highly with lifespan in a series of hypomorphic daf‐2 mutants of C. elegans. The overexpression of CeGSTP2‐2 in daf‐2 is abrogated in daf‐16; daf‐2 mutants, indicating that expression of the gst‐10 gene is modulated by insulin‐like growth factor signaling. To determine whether the relationship between CeGSTP2‐2 and lifespan is causal, we used RNAi to knock down CeGSTP2‐2. Treatment with gst‐10‐specific dsRNA decreased CeGSTP2‐2 protein in wild‐type N2 and in daf‐2 strains to an approximately equal level. The ability to conjugate 4‐HNE was similarly decreased by RNAi, suggesting that the increment of that activity in daf‐2 over N2 is due largely to the overexpression of CeGSTP2‐2. RNAi‐mediated knock‐down of CeGSTP2‐2 led to an increased susceptibility to 4‐HNE, paraquat, and heat shock, and to a shortening of lifespan by 13% in both N2 and daf‐2 strains. These results indicate that CeGSTP2‐2 significantly contributes to the maintenance of the soma, and that this function is augmented in daf‐2 mutants concordantly with other longevity assurance genes, probably via insulin‐like growth factor signaling.
CXCL12 overexpression and secretion by aging fibroblasts enhance human prostate epithelial proliferation in vitro
Authors: Begley Lesa , Monteleon Christine , Shah Rajal B. , MacDonald James W. , Macoska Jill A. 
Published: November 2005

SummaryThe direct relationship between the aging process and the incidence and prevalence of both benign prostatic hyperplasia (BPH) and prostate cancer (PCa) implies that certain risk factors associated with the development of both diseases increase with the aging process. In particular, both diseases share an overly proliferative phenotype, suggesting that mechanisms that normally act to suppress cellular proliferation are disrupted or rendered dysfunctional as a consequence of the aging process. We propose that one such mechanism involves changes in the prostate microenvironment, which ‘evolves’ during the aging process and disrupts paracrine interactions between epithelial and associated stromal fibroblasts. We show that stromal fibroblasts isolated from the prostates of men 63–81 years of age at the time of surgery express and secrete higher levels of the CXCL12 chemokine compared with those isolated from younger men, and stimulate CXCR4‐mediated signaling pathways that induce cellular proliferation. These studies represent an important first step towards a mechanistic elucidation of the role of aging in the etiology of benign and malignant prostatic diseases.