bluuebunny/crossref_metadata_2025_split huggingface dataset:
A wicked problem: Systemic issues surrounding Canadian equestrian dressage and dressage horse welfare
Authors: Ross Megan , Proudfoot Kathryn , Merkies Katrina , Lundgren Charlotte , Ritter Caroline 
Published: February 2025

Abstract
Competitive dressage’s social licence to operate is in jeopardy due to ethical concerns surrounding the use of horses for dressage. There is limited research that contributes to our understanding of Canadian equestrian perspectives on the use of horses in dressage. The objectives of this study were to: (1) explore the cultural context of the Canadian dressage industry, including how horse well-being is integrated within the culture; and (2) investigate coaches’ and riders’ perceptions and experiences with the use of horses for dressage. An ethnographic case study approach was employed, where MR spent 2–6 weeks with each of the four participating Equestrian Canada Certified dressage coaches and their riders (at least four riders per coach for a total of 19 riders). Data collection included direct observation, recording field notes and conducting at least one in-depth interview with each coach and rider. Interviews and field notes were analysed using reflexive thematic analysis leading to the development of three themes: (1) the systems that participants operate within; (2) how these systems foster a culture of contradiction in the industry; and (3) the ‘equestrian dilemma’ highlighting how participants navigate their love for horses with their horses’ well-being amid the sport’s demands. The three themes portray that the issues faced by the dressage industry may be rooted in systemic problems and could be described as a ‘wicked problem’. These results aim to inform future research initiatives that promote a holistic understanding of the challenges faced by the dressage industry and promote systems thinking solutions.
The philosophical foundations of digital twinning
Authors: Wagg David J , Burr Christopher , Shepherd Jason , Xuereb Conti Zack , Enzer Mark , Niederer Steven 
Published: February 2025

Abstract
Digital twins are a new paradigm for our time, offering the possibility of interconnected virtual representations of the real world. The concept is very versatile and has been adopted by multiple communities of practice, policymakers, researchers, and innovators. A significant part of the digital twin paradigm is about interconnecting digital objects, many of which have previously not been combined. As a result, members of the newly forming digital twin community are often talking at cross-purposes, based on different starting points, assumptions, and cultural practices. These differences are due to the philosophical world-view adopted within specific communities. In this paper, we explore the philosophical context which underpins the digital twin concept. We offer the building blocks for a philosophical framework for digital twins, consisting of 21 principles that are intended to help facilitate their further development. Specifically, we argue that the philosophy of digital twins is fundamentally holistic and emergentist. We further argue that in order to enable emergent behaviors, digital twins should be designed to reconstruct the behavior of a physical twin by “dynamically assembling” multiple digital “components”. We also argue that digital twins naturally include aspects relating to the philosophy of artificial intelligence, including learning and exploitation of knowledge. We discuss the following four questions (i) What is the distinction between a model and a digital twin? (ii) What previously unseen results can we expect from a digital twin? (iii) How can emergent behaviours be predicted? (iv) How can we assess the existence and uniqueness of digital twin outputs?
Supporting Breastfeeding Women Amid Natural Disasters in the US: A Scoping Review
Authors: Russell Jennifer H. , Haushalter Alisa , Rhoads Sarah J. 
Published: February 2025

Abstract

Objectives
Mothers, particularly those who are breastfeeding, as well as their babies are often overlooked populations during natural disasters. Still, the ever-growing frequency and severity of natural disasters in the United States (US) have increased the likelihood that a breastfeeding family will experience a natural disaster. In 2019, 83% of all newborns received breastmilk, and 84% of Americans lived in an area that recently experienced a natural disaster. This scoping review aimed to identify ways to support breastfeeding women, infants, and young children during a natural disaster.

Methods
A search was performed using Embase, Scopus, PubMed, and CINHAL databases to identify articles published in the English language from September 2005—September 2023 according to the Joanna Briggs Institute (JBI). Each article was chosen based on noted inclusion and exclusion criteria. Data were extracted per the pre-prepared protocol.

Results
Ten articles were selected for the scoping review, examining key characteristics and recommendations. The authors of the included articles used varying approaches to present the information, and differing approaches to supporting breastfeeding during disasters were observed. Only 3 of the 10 articles were research studies, 3 were editorials, 2 were program descriptions or evaluations, 1 was an education article, and 1 was a report. All articles addressed women, pregnant or postpartum women, infants, and/or young children. All articles addressed hurricanes, and 1 article addressed 2 disasters: a winter storm and a hurricane.

Conclusions
Recommendations include the adoption of breastfeeding women, infants, and young children in disaster plans as a special population with unique needs, integration of breastfeeding support into disaster plans, and training disaster workers to support breastfeeding during disasters.
BRITAIN’S TRADE CHALLENGE: TRACKING THE COSTS IN REAL TIME
Authors: Davies Richard , Hellings Josh 
Published: February 2025

Abstract
Since early 2021, food prices in Britain have increased by 30%. Using monthly microdata, researchers have found that frictions in the UK’s new trade relationship with the European Union (EU) play an important part in this inflation. The trade relationship is evolving, with further changes expected in 2024. This article establishes a framework for identifying trade-related inflation in close to real time. Using programming techniques, we collect daily prices of over 100,000 supermarket items, covering 80% of the UK grocery market. We identify 1,200 products from 12 countries with a protected designation of origin (PDO). This allows us to link price changes to individual EU economies. Addressing the predominance of EU PDOs, we employ a large language model to discern product origins from additional web-scraped data, thus broadening our analysis to cover over 67,000 products. Since August 2023, we find that prices for EU-originating food products have increased at a rate of 50% higher than domestically sourced products. This study presents a unique methodological approach to dissecting food sector inflation, which is well-positioned to be used in a policy setting, allowing us to assess the possible impact of impending nontariff barriers at the GB-EU border in 2024.
Assessing bilingual language proficiency with a yes/no vocabulary test: the role of form-meaning vocabulary knowledge
Authors: Lee Soon Tat , van Heuven Walter J. B. , Price Jessica M. , Leong Christine X. R. 
Published: February 2025

Abstract
Validated yes/no vocabulary tests that measure bilinguals’ language proficiency based on vocabulary knowledge have been widely used in psycholinguistic research. However, it is unclear what aspects of test takers’ vocabulary knowledge are employed in these tests, which makes the interpretation of their scores problematic. The present study investigated the contribution of bilinguals’ form-meaning knowledge to their item accuracy on a Malay yes/no vocabulary test. Word knowledge of Malay first- (N = 80) and second-language (N = 80) speakers were assessed using yes/no, meaning recognition, form recognition, meaning recall and form recall tests. The findings revealed that 59% of the variance in the yes/no vocabulary test score was explained by the accuracy of the meaning recognition, form recognition and meaning recall tests. Importantly, the item analysis indicated that yes/no vocabulary tests assess primarily knowledge of form recognition, supporting its use as a lexical proficiency measure to estimate bilinguals’ receptive language proficiency.
Established native hedgerows on field borders suppress weeds on farms
Authors: Long Rachael Freeman , Valliere Justin Michael 
Published: February 2025

Abstract
Established hedgerows of native plants on the borders of crop fields provide a variety of ecosystem service benefits in agricultural landscapes. However, their influence on weed communities is not well understood, and there are concerns that hedgerows could contribute to weed infestations on farms. To address this research gap, we examined the role of established hedgerows of native California plants on weed abundance (weed numbers and cover) and weed species richness in field borders and in adjacent crops, in large-scale, monocropping systems compared with conventionally managed field borders (i.e., no hedgerows). Across 20 farm sites in California’s Central Valley, hedgerows on orchard crop borders reduced weed numbers by 66%, weed species richness by 59%, and weed cover by 74%. On annual field crop borders, hedgerows reduced weed numbers by 71%, weed species richness by 60%, and weed cover by 70%. In orchards, hedgerows also reduced weed intrusion into the adjacent crop interior, with significantly lower weed cover to the first tree row (area directly underneath the trees), weed species richness to the 10 m tree row, and weed numbers to the 10 m avenue (area between the tree rows). Yearly management practices and associated costs for weed control in established hedgerows were significantly less than for conventionally managed field borders. This study highlights the effectiveness of native hedgerows as a sustainable nature-based solution for reducing weed pressure and management inputs on farms.
Agronomic predictors of herbicide-resistance in horseweed (Erigeron canadensis) for proactive management in Michigan
Authors: Sulzback Juliano R. M. , Patterson Eric L. , Burns Erin E. 
Published: February 2025

Abstract
Herbicide-resistant weeds threaten modern agriculture production. In Michigan, horseweed (Erigeron canadensis L.; syn. Conyza canadensis (L.) Cronq.) is among the most troublesome weeds, and glyphosate was widely used to control E. canadensis. Due to extreme selection pressure imposed by heavy glyphosate usage, glyphosate-resistant E. canadensis is widespread. New technologies to control resistant E. canadensis are being introduced in the form of multiple herbicide-resistance traits into glyphosate-resistant soybean (e.g. dicamba or 2,4-D choline). These new soybean [Glycine max (L.) Merr.] varieties will likely increase the use of 2,4-D and dicamba thus increasing the resistance selection pressure in E. canadensis. Predicting agronomic factors that drive herbicide-resistance evolution can serve as an effective proactive tool to advise practitioners to modify management strategies. Therefore, the objectives of this study are: 1) conduct dose-response assays to assess current resistance spectrum of E. canadensis collected in Michigan and 2) predict and determine the main factors in row crop production that contribute to resistance evolution in these accessions. Dose-response assays were conducted to evaluate the herbicide sensitivity spectrum to glyphosate, dicamba, and 2,4-D in 20 E. canadensis accessions collected from eight Michigan counties. Out of the 20 accessions, 60% were resistant to glyphosate, 35% to 2,4-D, and 20% to dicamba. Pearson’s correlation coefficient of dose-response values were positive in all comparisons (2,4-D-dicamba, r = 0.35; dicamba-glyphosate, r = 0.15; 2,4-D-glyphosate, r = 0.21). Dose-response data were integrated in odds ratio analyses to access the influence that previous management history had on the occurrence of resistance. Out of the significant pairwise comparisons, 44% were related to crop rotation frequency, 33% to previous herbicide-resistance status, and 22% to location collected. Results highlight that growers have the ability to proactively manage herbicide-resistance evolution progression of E. canadensis in Michigan by adopting integrated weed management techniques to slow successive selection events that occur in low diversity management systems.
AXL signaling in cancer: from molecular insights to targeted therapies
Authors: Yadav Monika , Sharma Akansha , Patne Ketki , Tabasum Saba , Suryavanshi Jyoti , Rawat Laxminarayan , Machaalani Marc , Eid Marc , Singh Rana P. , Choueiri Toni K. , Pal Soumitro , Sabarwal Akash 
Published: February 2025

Abstract
AXL, a member of the TAM receptor family, has emerged as a potential target for advanced-stage human malignancies. It is frequently overexpressed in different cancers and plays a significant role in various tumor-promoting pathways, including cancer cell proliferation, invasion, metastasis, epithelial–mesenchymal transition (EMT), angiogenesis, stemness, DNA damage response, acquired therapeutic resistance, immunosuppression, and inflammatory responses. Beyond oncology, AXL also facilitates viral infections, including SARS-CoV-2 and Zika highlighting its importance in both cancer and virology. In preclinical models, small-molecule kinase inhibitors targeting AXL have shown promising anti-tumorigenic potential. This review primarily focuses on the induction, regulation and biological functions of AXL in mediating these tumor-promoting pathways. We discuss a range of therapeutic strategies, including recently developed small-molecule tyrosine kinase inhibitors (TKIs), monoclonal antibodies, and antibody–drug conjugates (ADCs), anti-AXL-CAR, and combination therapies. These interventions are being examined in both preclinical and clinical studies, offering the potential for improved drug sensitivity and therapeutic efficacy. We further discuss the mechanisms of acquired therapeutic resistance, particularly the crosstalk between AXL and other critical receptor tyrosine kinases (RTKs) such as c-MET, EGFR, HER2/HER3, VEGFR, PDGFR, and FLT3. Finally, we highlight key research areas that require further exploration to enhance AXL-mediated therapeutic approaches for improved clinical outcomes.
Src inhibition potentiates MCL-1 antagonist activity in acute myeloid leukemia
Authors: Hu Xiaoyan , Li Lin , Nkwocha Jewel , Kmieciak Maciej , Shang Shengzhe , Cowart L. Ashley , Yue Yang , Horimoto Katsuhisa , Hawkridge Adam , Rijal Arjun , Mauro Adolfo G. , Salloum Fadi N. , Hazlehurst Lori , Sdrimas Konstantinos , Moore Zackary , Zhou Liang , Ginder Gordon D. , Grant Steven 
Published: February 2025

Abstract
The importance of MCL-1 in leukemogenesis has prompted development of MCL-1 antagonists e.g., S63845, MIK665. However, their effectiveness in acute myeloid leukemia (AML) is limited by compensatory MCL-1 accumulation via the ubiquitin proteasome system. Here, we investigated mechanisms by which kinase inhibitors with Src inhibitory activity e.g., bosutinib (SKI-606) might circumvent this phenomenon. MCL-1 antagonist/SKI-606 co-administration synergistically induced apoptosis in diverse AML cell lines. Consistently, Src or MCL-1 knockdown with shRNA markedly sensitized cells to MCL-1 inhibitors or SKI-606 respectively, while ectopic MCL-1 expression significantly diminished apoptosis. Mechanistically, MCL-1 antagonist exposure induced MCL-1 up-regulation, an event blocked by Src inhibitors or Src shRNA knock-down. MCL-1 down-regulation was associated with diminished transcription and increased K48-linked degradative ubiquitination. Enhanced cell death depended functionally upon down-regulation of phosphorylated STAT3 (Tyr705/Ser727) and cytoprotective downstream targets c-Myc and BCL-xL, as well as BAX/BAK activation, and NOXA induction. Importantly, the Src/MCL-1 inhibitor regimen robustly killed primary AML cells, including primitive progenitors, but spared normal hematopoietic CD34+ cells and human cardiomyocytes. Notably, the regimen significantly improved survival in an MV4-11 cell xenograft model, while reducing tumor burden in two patient-derived xenograft (PDX) AML models and increased survival in a third. These findings argue that Src inhibitors such as SKI-606 potentiate MCL-1 antagonist anti-leukemic activity in vitro and in vivo by blocking MCL-1 antagonist-mediated cytoprotective MCL-1 accumulation by promoting degradative ubiquitination, disrupting STAT-3-mediated transcription, and inducing NOXA-mediated MCL-1 degradation. They also suggest that this strategy may improve MCL-1 antagonist efficacy in AML and potentially other malignancies.
Spatiotemporal transcriptomics elucidates the pathogenesis of fulminant viral myocarditis
Authors: Li Huihui , Chen Xueting , Wang James Jiqi , Shen Juan , Abuduwufuer Kudusi , Zhang Zhao , Dong Zhensheng , Wen Zheng , He Jingwei , Chen Silian , Li Wanshun , Chen Chen , Li Fan , Fang Xiaodong , Wang Dao Wen 
Published: February 2025

Abstract
Fulminant myocarditis (FM) is a severe inflammatory condition of the myocardium that often results in sudden death, particularly in young individuals. In this study, we employed single-nucleus and spatial transcriptomics to perform a comprehensive analysis of coxsackievirus B3 (CVB3)-induced FM in A/J mice, spanning seven distinct time points pre- and post-treatment. Our findings reveal that mesothelial cells play a critical role in the early stage of myocarditis by acting as primary targets for CVB3 infection. This triggers the activation of macrophages, initiating a cascade of inflammation. Subsequently, pro-inflammatory Inflammatory_Mac and T cells infiltrate the myocardium, driving tissue damage. We also identified Cd8+ effector T cells as key mediators of cardiomyocyte injury. These cells release cytotoxic molecules, particularly IFN-γ, which modulates the expression of Spi1, a factor implicated in exacerbating cardiomyocyte death and amplifying disease progression. Therapeutic interventions targeting the IFN-γ/Spi1 axis demonstrated significant efficacy in FM models. Notably, intravenous immunoglobulin (IVIG) treatment reduced mortality, suppressed viral proliferation, and mitigated the hyperinflammatory state of FM. IVIG therapy also downregulated IFN-γ and Spi1 expression, underscoring its immunomodulatory and therapeutic potential. This comprehensive spatiotemporal transcriptomic analysis provides profound insights into the pathogenesis of FM and highlights actionable therapeutic targets, paving the way for more effective management strategies for this life-threatening condition.
Natural selection and adaptive traits in the Maniq, a nomadic hunter-gatherer society from Mainland Southeast Asia
Authors: Herzog Tobias , Larena Maximilian , Kutanan Wibhu , Lukas Helmut , Fieder Martin , Schaschl Helmut 
Published: February 2025

Abstract
Asia is home to diverse hunter-gatherer populations characterized by significant morphological, anthropological, cultural, and linguistic diversity. Despite their importance in understanding ancestral human subsistence, little is known about the essential genetic adaptations of these groups. This study investigates the evolutionary pressures shaping the genome of the Maniq population, a nomadic hunter-gatherer group inhabiting the rainforests of southern Thailand. Using genome-wide approaches, including iHS, xp-EHH, PBE, and beta statistics, we identified signatures of positive and balancing selection. Genes under positive selection were enriched in pathways related to immunity, metabolic regulation, structural adaptation, cardiovascular performance, and neuromodulatory traits. Several genes associated with the Southeast Asian ‘negrito-like’ phenotype were also under positive selection. Balancing selection was primarily detected in immune-related genes, particularly within the HLA region, underscoring the critical role of genetic diversity in surviving pathogen-rich environments. Additionally, balancing selection in olfactory receptor genes highlights their importance in environmental sensing and adaptation. These results reveal the intricate interplay of positive and balancing selection in shaping the genetic landscape of the Maniq population and highlight their adaptations to the ecological and lifestyle challenges of life in the rainforest. This study contributes to our understanding of human evolutionary processes in tropical environments and hunter-gatherer societies.
Tracking the genetic diversity of SARS-CoV-2 variants in Nicaragua throughout the COVID-19 pandemic
Authors: Alemán Gerald Vásquez , Cerpas Cristhiam , Juarez Jose G. , Moreira Hanny , Arguello Sonia , Coloma Josefina , Harris Eva , Gordon Aubree , Bennett Shannon N. , Balmaseda Ángel 
Published: February 2025

Abstract
The global circulation of SARS-CoV-2 has been extensively documented; however, the dynamics within Central America, particularly Nicaragua, remain underexplored. This study characterizes the genomic diversity of SARS-CoV-2 in Nicaragua from March 2020 through December 2022, utilizing 1064 genomes obtained via next-generation sequencing. These sequences were selected nationwide and analyzed for variant classification, lineage predominance, and phylogenetic diversity. We employed both Illumina and Oxford Nanopore Technologies for all sequencing procedures. Results indicated a temporal and spatial shift in dominant lineages, initially from B.1 and A.2 in early 2020 to various Omicron subvariants toward the study’s end. Significant lineage shifts correlated with changes in COVID-19 positivity rates, underscoring the epidemiological impact of variant dissemination. Comparative analysis with regional data underscored the low diversity of circulating lineages in Nicaragua and their delayed introduction compared to other countries in the Central American region. The study also linked specific viral mutations with hospitalization rates, emphasizing the clinical relevance of genomic surveillance. This research advances the understanding of SARS-CoV-2 evolution in Nicaragua and provides valuable information regarding its genetic diversity for public health officials in Central America. We highlight the critical role of ongoing genomic surveillance in identifying emergent lineages and informing public health strategies.
Full-length inhibitor protein is the most effective to perturb human dUTPase activity
Authors: Kőhegyi Bianka , Tóth Zoé S. , Gál Enikő , Laczkovich Máté , Benedek András , Vértessy Beáta G. , Nyíri Kinga 
Published: February 2025

Abstract
It has been demonstrated recently that knockout of the dUTPase enzyme leads to early embryonic lethality in mice. However, to explore the physiological processes arising upon the lack of dUTPase an effective and selective enzyme inhibitor is much needed. A highly specific and strong binding proteinaceous human dUTPase inhibitor described by us recently was a promising starting point to develop a molecular tool to study temporal and conditional dUTPase inhibition in cellulo. Towards this end we determined the 3D crystal structure of the crystallizable amino terminal domain of inhibitor protein, named StlNT in complex with the human dUTPase and designed several point mutants based on the structure to improve the inhibition effectivity. The effect of StlNT and a peptide derived from the full-length inhibitor on the activity of the human dUTPase was also tested. We showed that the C-terminal part of the Stl protein omitted from the crystal structure has an important role in the enzyme inhibition as the full-length Stl is needed to exert maximal inhibition on the human dUTPase.