bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular subtyping and the construction of a predictive model of colorectal cancer based on ion channel genes
Authors: Ai Lian-jie , Li Guo-dong , Chen Gang , Sun Zi-quan , Zhang Jin-ning , Liu Ming 
Published: April 2024

Abstract
Purpose
Colorectal cancer (CRC) is a highly heterogeneous malignancy with an unfavorable prognosis. The purpose of this study was to address the heterogeneity of CRC by categorizing it into ion channel subtypes, and to develop a predictive modeling based on ion channel genes to predict the survival and immunological states of patients with CRC. The model will provide guidance for personalized immunotherapy and drug treatment.

Methods
A consistent clustering method was used to classify 619 CRC samples based on the expression of 279 ion channel genes. Such a method was allowed to investigate the relationship between molecular subtypes, prognosis, and immune infiltration. Furthermore, a predictive modeling was constructed for ion channels to evaluate the ion channel properties of individual tumors using the least absolute shrinkage and selection operator. The expression patterns of the characteristic genes were validated through molecular biology experiments. The effect of potassium channel tetramerization domain containing 9 (KCTD9) on CRC was verified by cellular functional experiments.

Results
Four distinct ion channel subtypes were identified in CRC, each characterized by unique prognosis and immune infiltration patterns. Notably, Ion Cluster3 exhibited high levels of immune infiltration and a favorable prognosis, while Ion Cluster4 showed relatively lower levels of immune infiltration and a poorer prognosis. The ion channel score could predict overall survival, with lower scores correlated with longer survival. This score served as an independent prognostic factor and presented an excellent predictive efficacy in the nomogram. In addition, the score was closely related to immune infiltration, immunotherapy response, and chemotherapy sensitivity. Experimental evidence further confirmed that low expression of KCTD9 in tumor tissues was associated with an unfavorable prognosis in patients with CRC. The cellular functional experiments demonstrated that KCTD9 inhibited the proliferation, migration and invasion capabilities of LOVO cells.

Conclusions
Ion channel subtyping and scoring can effectively predict the prognosis and evaluate the immune microenvironment, immunotherapy response, and drug sensitivity in patients with CRC.
Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development
Authors: Mazzarella Rosane , Cañón-Beltrán Karina , Cajas Yulia N. , Hamdi Meriem , González Encina M. , da Silveira Juliano C. , Leal Claudia L. V. , Rizos D. 
Published: April 2024

Abstract
Background
Extracellular vesicles (EVs) present in oviductal (OF) and uterine fluid (UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes (LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabolism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as microRNAs (miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional mechanisms.

Results
This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and signaling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs (bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one (bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect different environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and implantation.

Conclusions
Our study suggests that miRNAs within OF- and UF-EVs could modulate bovine embryo development and quality, providing insights into the intricate maternal-embryonic communication that might be involved in modulating lipid metabolism, immune response, and implantation during early pregnancy.
A compendium of ruminant gastrointestinal phage genomes revealed a higher proportion of lytic phages than in any other environments
Authors: Wu Yingjian , Gao Na , Sun Chuqing , Feng Tong , Liu Qingyou , Chen Wei-Hua 
Published: April 2024

Abstract
Background
Ruminants are important livestock animals that have a unique digestive system comprising multiple stomach compartments. Despite significant progress in the study of microbiome in the gastrointestinal tract (GIT) sites of ruminants, we still lack an understanding of the viral community of ruminants. Here, we surveyed its viral ecology using 2333 samples from 10 sites along the GIT of 8 ruminant species.

Results
We present the Unified Ruminant Phage Catalogue (URPC), a comprehensive survey of phages in the GITs of ruminants including 64,922 non-redundant phage genomes. We characterized the distributions of the phage genomes in different ruminants and GIT sites and found that most phages were organism-specific. We revealed that ~ 60% of the ruminant phages were lytic, which was the highest as compared with those in all other environments and certainly will facilitate their applications in microbial interventions. To further facilitate the future applications of the phages, we also constructed a comprehensive virus-bacteria/archaea interaction network and identified dozens of phages that may have lytic effects on methanogenic archaea.

Conclusions
The URPC dataset represents a useful resource for future microbial interventions to improve ruminant production and ecological environmental qualities. Phages have great potential for controlling pathogenic bacterial/archaeal species and reducing methane emissions. Our findings provide insights into the virome ecology research of the ruminant GIT and offer a starting point for future research on phage therapy in ruminants.
Eating disorders and social media use among college students in Japan and China: a brief cross-sectional survey
Authors: Bai Yijing , Numata Noriko , Shimizu Eiji 
Published: April 2024

Abstract
Background
In recent years, new forms of media-social networking services (SNS)-such as Facebook and Sina Weibo have spread rapidly. Greater SNS use has been associated with greater body dissatisfaction, which in turn, is related to greater eating disorder (ED) symptom severity. In this study, we (1) investigated the relationships between ED tendencies, SNS use intensity, and body esteem and (2) examined the mediating role of body esteem in the relationship between SNS use intensity and ED tendencies among Japanese and Chinese students.

Methods
A total of 564 Japanese and Chinese college students aged 18–22 years were surveyed on their age and BMI, including self-filling questions from the Japanese and Chinese versions of the Eating Attitudes Test (EAT-26), SNS Intensity Scale, Body Esteem Scale for Adolescents and Adults (BESAA), Patient Health Questionnaire-9 (PHQ-9), and Generalized Anxiety Disorder-7 (GAD-7) scale.

Results
The proportion of students with a score of ≥ 20 on the EAT-26 was 15.8% (Japanese, 14.7%; Chinese, 17.0%). The number of participants with scores ≥ 20 on the EAT-26 was significantly higher than ever before, both in Japan and China. Chinese students reported greater body esteem than Japanese students, as well as a stronger association of SNS use intensity with body esteem. Among Japanese students, EAT-26 scores were unrelated to SNS Intensity Scale scores but had a weak negative correlation with BESAA scores; therefore, body esteem did not mediate the relationship between SNS use intensity and ED tendencies. Among Chinese students, scores on the SNS Intensity Scale and BESAA had a weak correlation with EAT-26 scores, and SNS use intensity reduced ED tendencies through greater body esteem.

Conclusions
It is important to consider the way users engage with SNS, in addition to the SNS use intensity. Improving body esteem may reduce the risk of ED. Furthermore, it is necessary to include men in the discussion on ED in the future.
Shaping the future of preclinical development of successful disease-modifying drugs against Alzheimer's disease: a systematic review of tau propagation models
Authors: Basheer Neha , Buee Luc , Brion Jean-Pierre , Smolek Tomas , Muhammadi Muhammad Khalid , Hritz Jozef , Hromadka Tomas , Dewachter Ilse , Wegmann Susanne , Landrieu Isabelle , Novak Petr , Mudher Amritpal , Zilka Norbert 
Published: April 2024

AbstractThe transcellular propagation of the aberrantly modified protein tau along the functional brain network is a key hallmark of Alzheimer's disease and related tauopathies. Inoculation-based tau propagation models can recapitulate the stereotypical spread of tau and reproduce various types of tau inclusions linked to specific tauopathy, albeit with varying degrees of fidelity. With this systematic review, we underscore the significance of judicious selection and meticulous functional, biochemical, and biophysical characterization of various tau inocula. Furthermore, we highlight the necessity of choosing suitable animal models and inoculation sites, along with the critical need for validation of fibrillary pathology using confirmatory staining, to accurately recapitulate disease-specific inclusions. As a practical guide, we put forth a framework for establishing a benchmark of inoculation-based tau propagation models that holds promise for use in preclinical testing of disease-modifying drugs.
Experimental study on weathering mechanism of ancient bricks in Jiayuguan Wei-Jin tombs, Gansu, China
Authors: An Lihong , Qiao Zhen , Wang Jie , Wang Fengrui 
Published: April 2024

Abstract
This study focuses on the ancient bricks of Wei-Jin tombs in Jiayuguan, Gansu, China, analyzing the deterioration of the bricks under the long-term influence of natural environments and human activities. Currently, the ancient bricks exhibit various degradation diseases such as cracks, exfoliation, fracture, weathering, and microbial erosion, severely affecting the integrity of the cultural relics. Through on-site investigation and characterization testing, the physical and mechanical properties, compositional elements, pore size distribution, and thermal characteristics of the ancient bricks were analyzed. Indoor simulation experiments were conducted to study the impact of different types of environmental erosion cycles (such as H2O, HCl, NaOH, Na2SO4) on the performance and structure of the ancient bricks, the patterns and causes of deterioration were also studied. The results indicate that the cyclic effects gradually transform the porosity of the ancient bricks into lateral microcracks, which continue to expand, leading to varying degrees of degradation of performance. The extent of the impact of these cycles on the properties of ancient bricks is in descending order: Na2SO4, HCl, NaOH, H2O. H2O, NaOH, HCl, Na2SO4.
Who are the “police” in “police violence”? Fatal violence by U.S. law enforcement agencies across levels of government
Authors: Jahn Jaquelyn L. , Schwartz Gabriel L. 
Published: April 2024

Abstract
Background
Police violence is increasingly recognized as an urgent public health problem. Basic questions about police violence, however, remain unanswered, including which types of law enforcement agency are responsible for fatal police violence deaths.

Methods
We estimated the proportion of police violence deaths in the U.S. (2013–2022) that were attributable to local, county, state, federal, or tribal police agencies, using mapping police violence data. We examined proportions overall, by decedent race/ethnicity, and by state.

Results
Nationally, 60% of decedents were killed by municipal, 29% by county, 8% by state, and 3% by federal, police, with < 1% killed by tribal or other officers. These proportions varied by race/ethnicity, with 56% of Native American decedents killed by municipal police compared to 70–75% among other racially minoritized people. While municipal police were responsible for most deaths in most states, in the Southeast, county police predominated. In some Northeastern states (and Alaska), state police were responsible for > 40% of deaths.

Conclusions
We identify wide geographic & racial/ethnic variation in the agencies responsible for fatal police violence. Findings suggest that the budgetary and infrastructural shifts required to prevent fatal police violence need to occur at multiple levels of government.
Cx43 hemichannels and panx1 channels contribute to ethanol-induced astrocyte dysfunction and damage
Authors: Gómez Gonzalo I. , Alvear Tanhia F. , Roa Daniela A. , Farias-Pasten Arantza , Vergara Sergio A. , Mellado Luis A. , Martinez-Araya Claudio J. , Prieto-Villalobos Juan , García-Rodríguez Claudia , Sánchez Natalia , Sáez Juan C. , Ortíz Fernando C. , Orellana Juan A. 
Published: April 2024

Abstract
Background
Alcohol, a widely abused drug, significantly diminishes life quality, causing chronic diseases and psychiatric issues, with severe health, societal, and economic repercussions. Previously, we demonstrated that non-voluntary alcohol consumption increases the opening of Cx43 hemichannels and Panx1 channels in astrocytes from adolescent rats. However, whether ethanol directly affects astroglial hemichannels and, if so, how this impacts the function and survival of astrocytes remains to be elucidated.

Results
Clinically relevant concentrations of ethanol boost the opening of Cx43 hemichannels and Panx1 channels in mouse cortical astrocytes, resulting in the release of ATP and glutamate. The activation of these large-pore channels is dependent on Toll-like receptor 4, P2X7 receptors, IL-1β and TNF-α signaling, p38 mitogen-activated protein kinase, and inducible nitric oxide (NO) synthase. Notably, the ethanol-induced opening of Cx43 hemichannels and Panx1 channels leads to alterations in cytokine secretion, NO production, gliotransmitter release, and astrocyte reactivity, ultimately impacting survival.

Conclusion
Our study reveals a new mechanism by which ethanol impairs astrocyte function, involving the sequential stimulation of inflammatory pathways that further increase the opening of Cx43 hemichannels and Panx1 channels. We hypothesize that targeting astroglial hemichannels could be a promising pharmacological approach to preserve astrocyte function and synaptic plasticity during the progression of various alcohol use disorders.
Evaluation of Orexin-A Salivary Levels and its Correlation with Attention After Non-invasive Brain Stimulation in Female Volleyball Players
Authors: Moscatelli Fiorenzo , Monda Antonietta , Messina Antonietta , Monda Marcellino , Monda Vincenzo , Villano Ines , De Maria Antonella , Nicola Mancini , Marsala Gabriella , de Stefano Maria Ida , Limone Pierpaolo , Messina Giovanni , Polito Rita 
Published: April 2024

Abstract
Background
The capacity to change attention from one area to another depending on the many environmental circumstances present is a crucial aspect of selective attention and is strictly correlated to reaction time. The cholinergic system of the basal forebrain is crucial for attentive abilities. Several inputs, particularly orexin neurons, whose cell bodies are found in the postero-lateral hypothalamus, can activate the cholinergic system. The aim of this study was to investigate if high frequencies rTMS at dorsolateral prefrontal cortex (DLPFC) in highly trained volleyball players can change Orexin-A levels, attention and reaction time. This study was a double-blinded (participant and evaluator) matched-pair experimental design. Twenty right-handed female volleyball players were recruited for the study (age 24.6 ± 2.7 years; height 177.0 ± 5.5 cm; body mass 67.5 ± 6.5 kg; BMI 21.5 ± 1.2).

Results
The main finding of this study was that 10 Hz rTMS to the DLPFC seems to increase Orexin-A salivary levels and the percentage of correct answers, while decreasing RT. After rTMS, the athletes show an increase in the percentage of correct answers immediately after the end of stimulation, and also after 15 and 30 min. Moreover, the athletes show decreases in reaction time after the end of stimulation and after 15 and 30 min to the end of stimulation, while no differences were found at the end of stimulation. Finally, the athletes show significant increases in Orexin-A salivary levels after stimulation with a peak after 30’ of the end.

Conclusion
The results of our study seem to indicate that there is a relationship between salivary Orexin-A levels and RT. These results could provide useful tools for modulating sports training; in fact, if confirmed, they could lead coaches to offer their athletes rTMS sessions appropriately integrated with training. In fact, alternating attention is a mental flexibility that enables people to change their point of focus and switch between tasks requiring various levels of cognition.
Long-term cardiac effects of modern treatment for Hodgkin’s lymphoma
Authors: Bjerring Anders W , Smeland Knut HB , Stokke Thomas , Haugaa Kristina H , Holte Espen , Rösner Assami , Kiserud Cecilie E , Edvardsen Thor , Sarvari Sebastian Imre 
Published: April 2024

Abstract
Background
Hodgkin’s lymphoma (HL) is a hematological malignancy that affects both children and young adults. Traditional treatment is associated with a life-time prevalence of cardiac disease exceeding 50%. In the late 1990s protocols were modified to reduce cancer therapy-related adverse cardiac effects. This study aimed to assess the long-term impact of advances in treatment protocols on the cardiac health of HL survivors (HLS).

Methods
HLS (n = 246) treated between 1997 and 2007 with anthracycline-based chemotherapy in three centers in Norway were included. Of these, 132 (53%) had also received mediastinal radiotherapy. HLS were compared to controls (n = 58) recruited from the general population and matched for sex, age, smoking status, and heredity for coronary artery disease. All subjects underwent echocardiography, clinical assessment, and blood sampling.

Results
The HLS were 46 ± 9 years old and had been treated 17 ± 3 years before inclusion in the study. There was no significant difference between HLS and controls in ejection fraction (EF) (58%±5 vs. 59%±4, p = 0.08) or prevalence of heart failure. HLS treated with both anthracyclines and mediastinal radiotherapy (AC + MRT) had slightly worse left ventricular global longitudinal strain than controls (-19.3 ± 2.5% vs. -20.8 ± 2.0%, p < 0.001), but those treated with only anthracyclines did not. HLS treated with AC + MRT had a higher prevalence of valve disease than those treated only with anthracyclines (12% vs. 4%, p < 0.05).

Conclusions
HLS treated with anthracyclines after the late 1990s have similar cardiac function and morphology as age-matched controls, apart from higher rates of valvular disease in those who also underwent mediastinal radiotherapy.
Impact assessment of integrated-pathy on cancer-related fatigue in cancer patients: an observational study
Authors: Balkrishna Acharya , Katiyar Prashant , Ghosh Sourav , Singh Sumit Kumar , Arya Vedpriya 
Published: April 2024

Abstract
Background
Integrated-pathy aims to integrate modern medicine with traditional systems via applying the holistic approach of Ayurveda, Yoga, and natural medicine. This is important for addressing the challenges surrounding the delivery of long-term palliative care for chronic ailments including cancer. The prime intent of this study was to substantiate the underlying hypothesis behind the differential and integrative approach having a positive impact on Quality of Life of cancer patients.

Study design
Cross-sectional Observational study.

Methods
A standardized questionnaire was developed and used, after obtaining written informed consent from patients to assess the impact of Integrated-pathy on patients (n = 103) diagnosed with cancer receiving care at Patanjali Yoggram. The research was carried out over 8 months. All participants received a uniform treatment protocol as prescribed by Patanjali. For the sample size determination and validation, α and 1-β was calculated and for the significance of the pre- and post-treatment QoL ratings, Shapiro wilk test and other descriptive statistics techniques were explored.

Results
A total of 103 patients seeking cancer special-healthcare were interviewed, out of which 39 (37.86%) remained finally based on the inclusion/exclusion criteria with age (25–65 years), types of cancers (Carcinoma and Sarcoma), chemotherapy/radiotherapy received or not, before opting Integrated-pathy. Follow-ups revealed a significant increase in the QoL (17.91%) after receiving the integrated therapy over a course of at least 1 month. Further, a significant reduction in cancer-related pain followed by an increase in QoL index was reported in the patients. Shapiro–wilk test revealed significant pairing (p < 0.001) with validation of the model using test.

Conclusions
To bolster evidence-based backing for Integrated-pathy, there is a need for clearly delineated clinical indicators that are measurable and trackable over time. Clinical investigators are encouraged to incorporate Integrated-pathy into their proposed interventions and conduct analogous studies to yield sustained advantages in the long run.
Analyzing the impact of an MDG-Fund program on childhood malnutrition in Timor-Leste
Authors: Van der Spek L. , Sonneveld B. G. J. S. 
Published: April 2024

Abstract
Background
Timor-Leste’s food insecurity, propelled by political conflicts, a fragile economy and biophysical limitations that characterize mountainous Small Island Developing States (SIDS), is expressed in a high incidence (50%) of stunted children. Hence, the Millennium Development Goals Achievement Fund’s Joint Program’s (MDG-F JP) in 2009 was a timely intervention to reduce prevalence of underweight among under-fives. Since the impact of the program remains largely unclear, the current study investigates the contributions of the MDG-F JP on improving children’s nutritional status in Timor-Leste, in order to inform policymakers on how to make future programs more effective.

Methods
Using bivariate analyses and multiple linear regression models we analyzed Demographic and Health Survey (DHS) data from under-fives in 2009–2010 and 2016, combined with spatially explicit data from geographic information systems (GIS). The analyses generated trends and factors associated with undernutrition, which were used in a quasi-experimental setting to compare districts that received the MDG-F JP with similar districts that did not receive MDG-F JP interventions.

Results
A comprehensive set of factors dependent on seasons, locations, and individuals determine undernutrition in Timor-Leste. A positive impact of the MDG-F JP was found for the average severity of wasting, but not for stunting and underweight.

Conclusions
The findings reinforce the pressing need for integrated and cross-sectoral programs, aimed especially at agricultural workers, mothers, and children. The agricultural challenge is to sustainably select, produce and conserve higher-yield and nutrient-rich crops, and educational enhancement should be aligned with local practices and research.