bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomics- and proteomics-based multi-omics integration reveals early metabolite alterations in sepsis-associated acute kidney injury
Authors: Huang Pengfei , Liu Yanqi , Li Yue , Xin Yu , Nan Chuanchuan , Luo Yinghao , Feng Yating , Jin Nana , Peng Yahui , Wang Dawei , Zhou Yang , Luan Feiyu , Wang Xinran , Wang Xibo , Li Hongxu , Zhou Yuxin , Zhang Weiting , Liu Yuhan , Yuan Mengyao , Zhang Yuxin , Song Yuchen , Xiao Yu , Shen Lifeng , Yu Kaijiang , Zhao Mingyan , Cheng Lixin , Wang Changsong 
Published: February 2025

Abstract

Background

Sepsis-associated acute kidney injury (SA-AKI) is a frequent complication in patients with sepsis and is associated with high mortality. Therefore, early recognition of SA-AKI is essential for administering supportive treatment and preventing further damage. This study aimed to identify and validate metabolite biomarkers of SA-AKI to assist in early clinical diagnosis.

Methods
Untargeted renal proteomic and metabolomic analyses were performed on the renal tissues of LPS-induced SA-AKI and sepsis mice. Glomerular filtration rate (GFR) monitoring technology was used to evaluate real-time renal function in mice. To elucidate the distinctive characteristics of SA-AKI, a multi-omics Spearman correlation network was constructed integrating core metabolites, proteins, and renal function. Subsequently, metabolomics analysis was used to explore the dynamic changes of core metabolites in the serum of SA-AKI mice at 0, 8, and 24 h. Finally, a clinical cohort (28 patients with SA-AKI vs. 28 patients with sepsis) serum quantitative metabolomic analysis was carried out to build a diagnostic model for SA-AKI via logistic regression (LR).

Results
Thirteen differential renal metabolites and 112 differential renal proteins were identified through a multi-omics study of SA-AKI mice. Subsequently, a multi-omics correlation network was constructed to highlight five core metabolites, i.e., 3-hydroxybutyric acid, 3-hydroxymethylglutaric acid, creatine, myristic acid, and inosine, the early changes of which were then observed via serum time series experiments of SA-AKI mice. The levels of 3-hydroxybutyric acid, 3-hydroxymethylglutaric acid, and creatine increased significantly at 24 h, myristic acid increased at 8 h, while inosine decreased at 8 h. Ultimately, based on the identified core metabolites, we recruited 56 patients and constructed a diagnostic model named IC3, using inosine, creatine, and 3-hydroxybutyric acid, to early identify SA-AKI (AUC = 0.90).

Conclusions
We proposed a blood metabolite model consisting of inosine, creatine, and 3-hydroxybutyric acid for the early screening of SA-AKI. Future studies will observe the performance of these metabolites in other clinical populations to evaluate their diagnostic role.
Touchscreen-based assessment of upper limb kinematics after stroke: Reliability, validity and sensitivity to motor impairment
Authors: Goizueta Sandra , Navarro María Dolores , Calvo Gabriela , Campos Gloria , Colomer Carolina , Noé Enrique , Llorens Roberto 
Published: February 2025

Abstract

Background
Conventional clinical tools for assessing upper limb motor function often lack the sensitivity and specificity needed to detect subtle changes in motor performance and may be subject to bias. Kinematic assessment offers a potential solution by providing objective, precise, and detailed data on movement quality. However, it is typically associated with high costs, complex equipment, time-consuming procedures, and the need for controlled environments, all of which limit its accessibility and practicality in clinical settings. This study aimed to evaluate the reliability, validity, and sensitivity of a low-cost, touchscreen-based kinematic assessment tool for measuring upper limb function in individuals post-stroke.

Methods
Sixty-four individuals with stroke participated in this study. Participants performed a visually guided reaching task on a large touch screen that consisted in reaching from a central target to five outer targets arranged in a circular pattern, each at a time, and then returning to the central target. Their motor function was assessed using the Fugl-Meyer Assessment for Upper Extremity, the Box and Block Test, and the Nine Hole Peg Test. Kinematic measures of the trajectories performed during the reaching task were extracted and analyzed for reliability, convergent validity with clinical assessments, and sensitivity to impairment severity.

Results
The kinematic measures demonstrated good to excellent test-retest reliability, with intraclass correlation coefficients ranging from moderate to excellent. The convergent validity analysis revealed multiple significant correlations between the kinematic parameters and clinical assessments, particularly in tests requiring higher skill and precision, such as the Coordination and Speed subscale of the Fugl-Meyer Assessment for Upper Extremity and the Nine Hole Peg Test. Additionally, the touchscreen-based assessment was sensitive to the severity of motor impairment, as reflected by notable differences in the kinematic measures among participants with varying levels of upper limb function.

Conclusions
The touchscreen-based kinematic assessment offered an affordable yet reliable, valid, and sensitive alternative for evaluating upper limb kinematics in individuals with stroke, which could complement clinical assessments by offering additional insights into motor performance. Furthermore, its low cost, high speed, and ease of use make it a practical option for widespread clinical adoption.
From mechanisms to future therapy: a synopsis of isolated REM sleep behavior disorder as early synuclein-related disease
Authors: Stefani Ambra , Antelmi Elena , Arnaldi Dario , Arnulf Isabelle , During Emmanuel , Högl Birgit , Hu Michele M. T. , Iranzo Alex , Luke Russell , Peever John , Postuma Ronald B. , Videnovic Aleksandar , Gan-Or Ziv 
Published: February 2025

Abstract
Parkinson disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy are synucleinopathies, characterized by neuronal loss, gliosis and the abnormal deposition of α-synuclein in vulnerable areas of the nervous system. Neurodegeneration begins however several years before clinical onset of motor, cognitive or autonomic symptoms. The isolated form of REM sleep behavior disorder (RBD), a parasomnia with dream enactment behaviors and excessive muscle activity during REM sleep, is an early stage synucleinopathy. The neurophysiological hallmark of RBD is REM sleep without atonia (RWSA), i.e. the loss of physiological muscle atonia during REM sleep. RBD pathophysiology is not fully clarified yet, but clinical and basic science suggest that ɑ-syn pathology begins in the lower brainstem where REM atonia circuits are located, including the sublaterodorsal tegmental/subcoeruleus nucleus and the ventral medulla, then propagates rostrally to brain regions such as the substantia nigra, limbic system, cortex. Genetically, there is only a partial overlap between RBD, PD and DLB, and individuals with iRBD may represent a specific subpopulation. A genome-wide association study identified five loci, which all seem to revolve around the GBA1 pathway. iRBD patients often show subtle motor, cognitive, autonomic and/or sensory signs, neuroimaging alterations as well as biofluid and tissue markers of neurodegeneration (in particular pathologic α-synuclein aggregates), which can be useful for risk stratification. Patients with iRBD represent thus the ideal population for neuroprotective/neuromodulating trials. This review provides insights into these aspects, highlighting and substantiating the central role of iRBD in treatment development strategies for synucleinopathies.
Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility
Authors: Sommer Andrew J. , Deblois Courtney L. , Tu Andrew D. J. , Suen Garret , Coon Kerri L. 
Published: February 2025

Abstract
Stable flies in the genus Stomoxys are highly abundant, blood-feeding pests on dairy farms; however, their role in the carriage and potential transmission of pathogens is largely understudied. Here, we report on the frequency and distribution of culturable bacteria collected from Stomoxys flies captured in free stall barns and nearby calf hutches over a three-month period on a focal research farm in Wisconsin, USA. Mastitis-associated bacterial taxa, including Staphylococcus, Escherichia, Enterobacter, and Klebsiella spp., were frequently isolated from pooled samples of the internal or external portions of the flies. Conversely, selective enrichment protocols from these samples yielded only a single isolate of Salmonella and no enterohemorrhagic Escherichia coli O157. Neither trap location nor time of capture had a significant impact on the observed frequency of most bacterial genera isolated from the flies. Our results confirm that Stomoxys flies harbor both mastitis-associated bacterial taxa and bacterial taxa associated with opportunistic infections in humans. Further research into the transmission of fly-associated microbes could be important in the control of mastitis or other bacterial diseases on dairy farms.
Carboxylesterase 1-mediated endocannabinoid metabolism in skin: role in melanoma progression in BRafV600E/Pten−/− mice
Authors: Morozova Veronika , Pellegata Daniele , Charles Roch-Philippe , Gertsch Jürg 
Published: February 2025

Abstract

Background
Melanoma is a highly aggressive skin cancer with a poor prognosis. The endocannabinoids 2-arachidonoylgylcerol (2-AG) and anandamide have been linked to melanoma progression, though their roles remain unclear. We hypothesized that the 2-AG-arachidonate-prostaglandin axis could drive aggressive melanoma progression.

Methods
The genetically engineered melanoma mouse model B6-Tyr::CreER

T2

; BRaf

CA

; Pten

loxP
was characterized by targeted metabolomics. Functionally expressed serine hydrolases in the tumor tissue were identified by chemoproteomics. Pharmacological inhibition of carboxylesterase 1 (CES1) was achieved through chronic in vivo i.p. treatment with JZL184 (10 mg/kg daily), confirmed by activity-based protein profiling (ABPP) and targeted lipidomics. CES1-mediated 2-AG hydrolysis was further confirmed in radiotracer-based assays using CES1-transfected cell lines.

Results
The diacylglycerol and protein kinase C activator 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG) was significantly elevated in the nodular-like melanoma tissues, along with 2-AG and arachidonic acid (ARA), compared to normal skin. AEA and other N-acylethanolamines were decreased, while, notably, prostaglandin levels remained unchanged. Significant changes in the levels of neuromodulators and neurotransmitters, including serotonin and adenosine, were observed. Pronounced differences between serine hydrolase activity in normal skin and melanoma tissue were identified by ABPP. Intriguingly, CES1 was identified as the only 2-AG-hydrolyzing enzyme in this melanoma tissue, as MAGL and ABHD6/12 were not expressed. The MAGL inhibitor JZL184 also efficiently inhibited CES1 in vitro and in vivo, increasing glycerol esters and reducing tumor progression. Additionally, scRNA-seq data from previous studies revealed divergent MAGL/CES1 expression patterns across different human melanoma subtypes.

Conclusions
A role of CES1 expression in skin is demonstrated for the first time. Our study suggests that 2-AG degradation to arachidonate favors melanoma progression, either reflecting the carcinogenic role of ARA or that monoacylglycerols like 2-AG and/or other CES1 substrates may exert antitumor effects, indicating that CES1 could be a potential therapeutic target. CES1 expression and high SAG, 2-AG, and ARA levels may be a signature of specific BRAF-driven malignant melanoma subtypes which are associated with discrete metabolic adaptations.

Graphical Abstract
The relationship between self-esteem and social avoidance among university students: chain mediating effects of resilience and social distress
Authors: Shang Aili , Feng Liping , Yan Guoli , Sun Ling 
Published: February 2025

Abstract

Backgroud
This study aims to explore the relationships between self-esteem, resilience, social distress, and social avoidance among college students. It also examines the mediating roles of resilience and social distress in the relationship between self-esteem and social avoidance.

Methods
A convenience cluster sampling method was used to select all first-year students from a university in Yinchuan, Ningxia. Data were collected through an online survey administered via the WENJUANXING platform, which included a general information questionnaire, the Self-Esteem Scale, the Psychological Resilience Scale, and the Social Avoidance and Distress Scale. A total of 2513 first-year students completed the survey. SPSS 26.0 software was used to analyze the correlation between self-esteem, resilience, social distress and social avoidance, and the mediation model was tested by Mplus8.

Results
Self-esteem, resilience were negatively correlated with social distress scores (r = -0.411, p < 0.01; r =-0.387, p < 0.01, respectively). Self-esteem and resilience were negatively correlated with social avoidance scores (r = -0.437, p < 0.01; r = -0.379, p < 0.01, respectively). Social distress and social avoidance scores were positively correlated (r = 0.778, p < 0.01). Resilience partially mediated the association between self-esteem and social avoidance(β = -0.02, p < 0.01), with a mediation rate of 5.01%. Social distress partially mediated the associations between self-esteem and social avoidance(β = -0.203, p < 0.01) with a mediation rate of 50.87%. Resilience and social distress together (β = -0.06, p < 0.01) formed a mediating chain between self-esteem and social avoidance, with a mediation rate of 15.03%.

Conclusions
Self-esteem was negatively associated with social avoidance. Resilience and social distress were found to mediate the association partially.
The role of platelets in cancer: from their influence on tumor progression to their potential use in liquid biopsy
Authors: Morales-Pacheco Miguel , Valenzuela-Mayen Miguel , Gonzalez-Alatriste Angel M. , Mendoza-Almanza Gretel , Cortés-Ramírez Sergio A. , Losada-García Alberto , Rodríguez-Martínez Griselda , González-Ramírez Imelda , Maldonado-Lagunas Vilma , Vazquez-Santillan Karla , González-Covarrubias Vanessa , Pérez-Plasencia Carlos , Rodríguez-Dorantes Mauricio 
Published: February 2025

Abstract
Platelets, anucleate blood cells essential for hemostasis, are increasingly recognized for their role in cancer, challenging the traditional notion of their sole involvement in blood coagulation. It has been demonstrated that platelets establish bidirectional communication with tumor cells, contributing to tumor progression and metastasis through diverse molecular mechanisms such as modulation of proliferation, angiogenesis, epithelial-mesenchymal transition, resistance to anoikis, immune evasion, extravasation, chemoresistance, among other processes. Reciprocally, cancer significantly alters platelets in their count and composition, including mRNA, non-coding RNA, proteins, and lipids, product of both internal synthesis and the uptake of tumor-derived molecules. This phenomenon gives rise to tumor-educated platelets (TEPs), which are emerging as promising tools for the development of liquid biopsies. In this review, we provide a detailed overview of the dynamic roles of platelets in tumor development and progression as well as their use in diagnosis and prognosis. We also provide our view on current limitations, challenges and future research areas, including the need to design more efficient strategies for their isolation and analysis, as well as the validation of their sensitivity and specificity through large-scale and rigorous clinical trials. This research will not only enable the evaluation of their clinical viability but could also open new opportunities to enhance diagnostic accuracy and develop personalized treatments in oncology.
Influence of oxalic acid and CTAB on the structural and optical properties of nanocrystalline tungsten oxide synthesized via co-precipitation method
Authors: Neethu R. P. , Madhu G. 
Published: February 2025

Abstract
Herein, we report the oxalic acid and cetyl trimethyl ammonium bromide (CTAB) assisted co-precipitation synthesis of nanocrystalline tungsten oxide (WO3). Different annealing temperatures were selected systematically based on the thermo-gravimetric analysis (TGA) of the precursors. The high crystallinity of the samples was revealed from the intense and narrow X-ray diffraction (XRD) peaks. Oxalic acid-assisted WO3 showed a considerable reduction in crystallite size. The increase in crystallite size with annealing temperature was also evident in both samples. The change of surfactant and annealing temperature resulted in a modification of surface morphology that was identified using high-resolution resolution-scanning electron microscopy (HR-SEM). The formation of WO3 was further established by the Raman spectra of the samples. Size strain plot (SSP) analysis of the samples showed a decrease of microstrain with an increase in annealing temperature. The bandgap energy obtained from the diffused reflectance spectra of the samples showed a red shift with an increase in annealing temperature. The X-ray photoelectron spectroscopy (XPS) analysis confirmed the existence of a pure oxidation state of W6+ in oxalic acid-assisted WO3 and mixed oxidation states of W6+ and W5+ in CTAB-assisted WO3 samples. The mesoporous nature and specific surface area of the samples are inferred from Brunauer–Emmett–Teller (BET) analysis. The reduced crystallite size, stable oxidation state, and higher specific surface area of the oxalic acid-assisted WO3 samples suggest its possible use as a supercapacitor and photocatalyst.

Graphical Abstract
Tribological properties of epoxy matrix composites filled with particles of multicomponent titanium-based alloy
Authors: Bagliuk Gennadii , Baranovska Oksana , Varchenko Viktor , Buketov Andriy , Sapronov Oleksandr , Ivanchenko Sehhii 
Published: February 2025

Abstract
The article presents the results of a study on the effect of a dispersed filler, produced by thermal synthesis from a mixture of titanium hydride, ferrosilicomanganese, and boron carbide powders on the tribological characteristics of a polymer composite based on ED-20 epoxy diane oligomer. The filler was incorporated into the resin at concentration ranging from 5 to 40 parts of composite powder per 100 parts (by weight) of the epoxy oligomer. At a sliding speed of 0.5 m/s, the highest friction coefficient (µ) in the range of 0.55–0.6 was noted for the epoxy polymer without a filler, while an increase in the filler concentration in the epoxy polymer led to a noticeable decrease in the friction coefficient values. The lowest values of µ (0.32–0.35) were observed in composites with 5 and 10 wt.% filler. With an increase in the sliding speed up to 1 m/s at the stage of constant friction, the friction coefficient for unreinforced polymer and the composite with 5% filler reached values of 0.55–0.65 followed by a transition to catastrophic wear. For polymers with 10, 20, and 40 wt.% filler, composites with a higher content of the dispersed component were characterized by lower values of µ. The specific wear of composites decreased with the incorporation of cermet particles into the polymer and with an increased in its concentration in the polymer from 5 to 20%. However, when the filler content increases to 40%, the level of specific wear increases slightly.
Tumor dormancy and relapse: understanding the molecular mechanisms of cancer recurrence
Authors: Tufail Muhammad , Jiang Can-Hua , Li Ning 
Published: February 2025

Abstract
Cancer recurrence, driven by the phenomenon of tumor dormancy, presents a formidable challenge in oncology. Dormant cancer cells have the ability to evade detection and treatment, leading to relapse. This review emphasizes the urgent need to comprehend tumor dormancy and its implications for cancer recurrence. Despite notable advancements, significant gaps remain in our understanding of the mechanisms underlying dormancy and the lack of reliable biomarkers for predicting relapse. This review provides a comprehensive analysis of the cellular, angiogenic, and immunological aspects of dormancy. It highlights the current therapeutic strategies targeting dormant cells, particularly combination therapies and immunotherapies, which hold promise in preventing relapse. By elucidating these mechanisms and proposing innovative research methodologies, this review aims to deepen our understanding of tumor dormancy, ultimately facilitating the development of more effective strategies for preventing cancer recurrence and improving patient outcomes.
Preliminary outcomes of Neuroform Atlas stent-assisted coiling for intracranial aneurysms with small parent vessels
Authors: Xiao Jingrui , Li Tianli , Wan Dongdong , Zhou Qidi , Zhao Xiaolong , Zhang Zhaolong , Xie Yixing , Shao Liming , Liu Guoping , Sun Chengjian , Xu Rui 
Published: February 2025

Abstract

Background
Although stent-assisted coiling has become a standard approach for treating intracranial aneurysms (IAs), there are limited reports on its safety and effectiveness in parent artery less than 2.5 mm in diameter. This study evaluates the feasibility, safety, and short-term outcomes of using Neuroform Atlas stent-assisted coiling for IAs with small parent vessels.

Methods
This study reviewed and analyzed the clinical data of 50 IAs in 50 patients with a parent artery diameter of ≤ 2.5 mm, treated with Neuroform Atlas stent-assisted coiling at a single center between November 2020 and April 2024. Immediate postoperative angiographic outcomes were assessed using the modified Raymond-Roy classification. Follow-up imaging included computed tomographic angiography (CTA), magnetic resonance angiography (MRA), and digital subtraction angiography (DSA). Clinical outcomes were evaluated using the modified Rankin Scale (mRS).

Results
The procedures achieved a 100% success rate. Immediately after treatment, 24 cases were classified as Raymond-Roy grade I, 11 as grade II, and 15 as grade III. Follow-up angiography in 28 cases revealed three instances of aneurysm recurrence, with a secondary procedure performed in one case. One patient reported poor neurological status, and two cases experienced procedure-related adverse events during telephone or clinical follow-up.
Conclusions the Atlas stent demonstrated favorable outcomes in the treatment of aneurysms in small parent arteries (< 2.5 mm), with a low complication rate. The timely postoperative use of tirofiban may further reduce the risk of ischemic complications.