bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sheep stromal‐epithelial cell interactions and ovarian tumor progression
Authors: Wang‐Johanning Feng , Huang Miao , Liu Jinsong , Rycaj Kiera , Plummer Joshua B. , Barnhart Kirstin F. , Satterfield William C. , Johanning Gary L. 
Published: July 2007

AbstractPrevious studies suggest that underlying ovarian stromal cues may regulate the ovarian surface epithelium. However, little is known about the interaction between ovarian stromal cells (OSC) and ovarian surface epithelial cells (OSE) under normal physiologic and pathologic conditions, largely because of the lack of a suitable model. In the current study, the OSC obtained from a sheep were immortalized with SV‐40 T/t antigen (designated IOSC) and telomerase reverse transcriptase (designated IOSCH), followed by transfection with the oncogenic allele of the human H‐Ras oncogene (designated IOSChR). IOSC cells transfected with H‐Ras before immortalization with telomerase were designated IOSCRH. These sheep OSCs were used in both in vitro and in vivo model systems to evaluate mechanisms by which OSCs influence ovarian tumor progression. Normal sheep OSCs were found to inhibit the growth of SKOV3 and OVCAR3 human ovarian cancer cells, but not normal sheep OSE and human OSE cells (hOSE137 cells). In contrast, IOSChR and IOSCRH cells stimulated the growth of normal sheep and human OSE cells, as well as cancer cells. These findings were confirmed by in vivo studies. Our data provide compelling support for the importance of stromal‐epithelial cell interactions during tumor progression, and show for the first time that immortalized and transformed OSCs promote growth of ovarian epithelial tumors. © 2007 Wiley‐Liss, Inc.
Retracted: RNAi‐mediated downregulation of urokinase plasminogen activator receptor and matrix metalloprotease‐9 in human breast cancer cells results in decreased tumor invasion, angiogenesis and growth
Authors: Kunigal Sateesh , Lakka Sajani S. , Gondi Christopher S. , Estes Norman , Rao Jasti S. 
Published: July 2007

AbstractThe serine protease urokinase‐type plasminogen activator (uPA) plays a significant role in tumor cell invasion and metastasis when bound to its specific receptor, uPAR (also known as CD87). In addition to the uPA‐uPAR system, matrix metalloproteinases (MMPs) are involved in tumor cell invasion and metastasis. In this study, we achieved specific inhibition of uPAR and MMP‐9 using RNAi technology. We introduced small interfering RNA to downregulate the expression of uPAR and MMP‐9 (pUM) in breast cancer cell lines (MDA MB 231 and ZR 75 1). In vitro angiogenesis studies indicated a decrease in the angiogenic potential of the treated cells; in particular, a remarkable decrease was observed in the cells treated with bicistronic construct (pUM) in comparision to the controls. Additionally, bicistronic construct inhibited the formation of capillary‐like structures in in vivo models of angiogenesis. Similarly, the invasive potential and migration decreased dramatically when treated with the bicistronic construct as shown by matrigel invasion and migration assays. These results suggest a synergistic effect from the simultaneous downregulation of uPAR and MMP‐9. We also assessed the levels of phosphorylated forms of MAPK, ERK and AKT signaling pathway molecules and found reduction in the levels of these molecules in cells treated with the bicistronic construct as compared to the control cells. Furthermore, targeting both uPAR and MMP‐9 totally regressed orthotopic breast tumors in nude mice. In conclusion, our results provide evidence that the simultaneous downregulation of uPAR and MMP‐9 using RNAi technology may provide an effective tool for breast cancer therapy. © 2007 Wiley‐Liss, Inc.
The farnesyltransferase inhibitor R115777 (ZARNESTRA®) enhances the pro‐apoptotic activity of interferon‐α through the inhibition of multiple survival pathways
Authors: Caraglia Michele , Marra Monica , Viscomi Caterina , D'Alessandro Anna Maria , Budillon Alfredo , Meo Giuseppina , Arra Claudio , Barbieri Antonio , Rapp Ulf R , Baldi Alfonso , Tassone Pierfrancesco , Venuta Salvatore , Abbruzzese Alberto , Tagliaferri Pierosandro 
Published: July 2007

AbstractInterferon α (IFNα) induces an EGF‐Ras→Raf‐1→Erk dependent survival pathway counteracting apoptosis induced by the cytokine. In this paper we have evaluated the effects of the combination between farnesyl‐transferase inhibitor (FTI) R115777 and IFNα on the growth inhibition and apoptosis of cancer cells. Simultaneous exposure to R115777 and IFNα produced synergistic both antiproliferative and proapoptotic effects. In these experimental conditions, IFNα and R115777 completely antagonized the increased activity of both Ras and Erk‐1/2 induced by IFNα and strongly reduced Akt activity. Furthermore, treatment with R115777 in combination with IFNα regimen induced tumor growth delay on established KB cell xenografts in nude mice, while the single agents were almost inactive. R115777 was again able to antagonize the Ras‐dependent survival pathway induced by IFNα alsoin vivo. Raf‐1, one of the downstream targets of Ras, has been reported to activate bcl‐2 through displacement and/or phosphorylation of Bad. We have found that IFNα induced mitochondrial localization of Raf‐1 that was antagonized by R115777. Moreover, IFNα increased Raf‐1/bcl‐2 immuno‐conjugate formation and intracellular co‐localization and enhanced phosphorylation of Bad at Ser 112 and again R115777 counteracted all these effects. Moreover, the use of plasmids encoding for dominant negative or dominant positive Raf‐1 antagonized and potentiated, respectively, the co‐immunoprecipitation between Raf‐1 and bcl‐2. In conclusion, FTI R115777 strongly potentiates the antitumor activity of IFNα bothin vitroandin vivothrough the inhibition of different survival pathways that are dependent from isoprenylation of intracellular proteins such as ras. © 2007 Wiley‐Liss, Inc.
Hypoxia upregulates carcinoembryonic antigen expression in cancer cells
Authors: Kokkonen Nina , Ulibarri Ines Fernandez , Kauppila Annika , Luosujärvi Hanne , Rivinoja Antti , Pospiech Helmut , Kellokumpu Ilmo , Kellokumpu Sakari 
Published: July 2007

AbstractCarcinoembryonic antigen (CEA, ceacam5) is an important tumor‐associated antigen with reported roles, e.g., in immunological defense, cell adhesion, cell survival and metastasis. Its overexpression in cancer cells is known to involve transcriptional activation of the CEA gene, but the underlying molecular details remain unclear. Here, we show that hypoxia and intracellular alkalinization, 2 factors commonly found in solid tumors, increase CEA protein expression in breast (MCF‐7) and colorectal (CaCo‐2 and HT‐29) cancer cells. The increase was comparable (2–3‐fold) to that observed in colorectal carcinomas in vivo. CEA promoter analyses further revealed that this upregulation involves a known binding site for HIF‐1 transcription factor (5′‐ACGTG‐3′) within one of the CEA promoter's positive regulatory elements (the FP1 site; the E‐box). Accordingly, deletion or targeted mutagenesis of this motif rendered the CEA promoter unresponsive to hypoxia. Our chromatin immunoprecipitation data confirmed that endogenous HIF‐1α binds to the CEA promoter in hypoxic cells but not in normoxic cells. Moreover, overexpression of the hypoxia‐inducible factor (HIF‐1α) was sufficient to increase CEA protein expression in the cells. In contrast, c‐Myc, which is known to bind to the overlapping E‐box, did not potentiate HIF‐1α‐induced CEA expression. CEA overexpression in vivo was also found to coincide with the expression of carbonic anhydrase IX, a well‐known hypoxia marker. Collectively, these results define CEA as a hypoxia‐inducible protein and suggest an important role for the tumor microenvironmental factors in CEA overexpression during tumorigenesis. © 2007 Wiley‐Liss, Inc.
An improvement on covering triples by quadruples
Authors: Ji L. 
Published: July 2007

AbstractLet C(3,4,v) be the minimum number of four‐element subsets (called blocks or quadruples) of a v‐element set X, such that each three‐element subset of X is contained in at least one block. Let $L(3,4,v)=\lceil {v\over 4}\lceil{v-1\over 3}\lceil{v-2\over 2}\rceil\rceil\rceil$. Schönheim has obtained C(3,4,v) ≥  L(3,4,v). Further, Mills showed that C(3,4,v) = L(3,4,v) for v ≢ 7 (mod 12), and Hartman, Mills, and Mullin showed that C(3,4,v) = L(3,4,v) for v ≡ 7 (mod 12) with v ≥  52423 or v = 499. In this article, it is proved that C(3,4,v) = L(3,4,v) for all v ≡ 7 (mod 12) with an exception v = 7 and possible exceptions of v = 12k + 7, k ∈ {1,2,3,4,5,7,8,9,10,11,12,16,21,23,25,29}. © 2007 Wiley Periodicals, Inc. J Combin Designs 16: 231–243, 2008
Cyclooxygenase‐2 mRNA expression correlates with aromatase expression in human breast cancer
Authors: Salhab M. , Singh‐Ranger G. , Mokbel R. , Jouhra F. , Jiang W.G. , Mokbel K. 
Published: July 2007

AbstractIntroductionThe cyclooxygenase‐2 (COX‐2), responsible for the conversion of arachidonic acid into prostaglandin (PG) E2, is known to increase intracellular cAMP and estrogen production in malignant breast tissue. The aromatase enzyme complex is responsible for local production of estrogens in breast cancer. Increasing evidence supports a role for COX‐2 in upregulation of aromatase activity. The aim of this study was to examine the relationship between COX‐2 and aromatase mRNA expression in human breast cancer.MethodsA total of 160 breast samples (127 tumor tissues and 33 normal tissues) were analyzed. Levels of transcription were determined using real‐time quantitative PCR. COX‐2 and aromatase mRNA expression were normalized against CK19. Levels of expression of COX‐2 were correlated with those of aromatase using Pearson's correlation method.ResultsLevels of expression of COX‐2/CK19 of both benign and malignant tissues were positively correlated with aromatase/CK19 transcript levels (correlation coefficient = +0.536, P < 0.0001). When we compared levels of expression of both genes in malignant samples only, there was a highly significant positive correlation (r = +0.611, P < 0.00001).ConclusionThis study demonstrates a strong positive relationship between COX‐2 and aromatase mRNA expression, and lends further support to the hypothesis that COX‐2 is an upregulator of aromatase in breast tissue. J. Surg. Oncol. 2007;96:424–428. © 2007 Wiley‐Liss, Inc.
Computability of compact operators on computable Banach spaces with bases
Authors: Brattka Vasco , Dillhage Ruth 
Published: July 2007

AbstractWe develop some parts of the theory of compact operators from the point of view of computable analysis. While computable compact operators on Hilbert spaces are easy to understand, it turns out that these operators on Banach spaces are harder to handle. Classically, the theory of compact operators on Banach spaces is developed with the help of the non‐constructive tool of sequential compactness. We demonstrate that a substantial amount of this theory can be developed computably on Banach spaces with computable Schauder bases that are well‐behaved. The conditions imposed on the bases are such that they generalize the Hilbert space case. In particular, we prove that the space of compact operators on Banach spaces with monotone, computably shrinking, and computable bases is a computable Banach space itself and operations such as composition with bounded linear operators from left are computable. Moreover, we provide a computable version of the Theorem of Schauder on adjoints in this framework and we discuss a non‐uniform result on composition with bounded linear operators from right. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
On the complexity of the pancake problem
Authors: Yu Fuxiang 
Published: July 2007

AbstractWe study the computational complexity of finding a line that bisects simultaneously two sets in the two‐dimensional plane, calledthe pancake problem, using the oracle Turing machine model of Ko. We also study the basic problem of bisecting a set at a given direction. Our main results are:(1) The complexity of bisecting a nice (thick) polynomial‐time approximable set at a given direction can be characterized by the counting class #P.(2) The complexity of bisecting simultaneously two linearly separable nice (thick) polynomial‐time approximable sets can be characterized by the counting class #P.(3) For either of these two problems, without the thickness condition and the linear separability condition (for the two‐set case), it is arbitrarily hard to compute the bisector, even if it is unique. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Polyester‐based Copolymers for Biomaterials Fabrication
Authors: Koseva Neli , Kurcok Piotr , Adamus Grazyna , Troev Kolio , Kowalczuk Marek 
Published: July 2007

AbstractSummary: Results on synthesis of poly(3‐hydroxybutyrate)s possessing one or two hydroxyl groups at one terminus of the chain and carboxylic group at the other chain end are reported. These polymers were further functionalised via transesterification with dimethyl H‐phosphonate thus incorporating a reactive/biodegradable center in the polyester backbone. Block/star‐like copolymers composed of hydrophilic PEG and hydrophobic poly(3‐hydroxybutyrate) segments linked by phosphoester moiety were also obtained. Chemical structure and composition of the reaction products were analysed applying different spectroscopic techniques (1H, and 31P NMR, IR and ESI‐MS) and size exclusion chromatography was applied to describe molecular weight averages and distribution.
Biodegradable Polyesters for Tissue Engineering
Authors: Slomkowski Stanislaw 
Published: July 2007

AbstractSummary: Paper describes basic characteristics of synthesis and properties of aliphatic polyesters used for tissue engineering. Described is also synthesis of polyester containing block copolymers suitable for surface modification. Described are methods used for scaffold fabrication with required porosity. In particular, presented are methods according to which scaffolds are made from prefabricated polyester micro‐ and nanoparticles.
IntelliDrug Implant for Medicine Delivery in Alzheimer's Disease Treatment
Authors: Moscicka Aleksandra E. , Czarnecka Kinga , Ciach Tomasz 
Published: July 2007

AbstractSummary: IntelliDrug it is a denture implant containing a reservoir with drug and making enable a galantamine transport via buccal mucosa by means of iontophoresis. The in vitro experimental studies on drug passive and iontophoretic transport through porcine buccal mucosa were carrying out in horizontal two‐chamber permeation cell with silver electrodes. The drug mass flux was investigated. The influences of initial drug concentration and current density for transport velocity were defined.
Sleep‐related faciomandibular myoclonus: A sleep‐related movement disorder different from bruxism
Authors: Loi Daniela , Provini Federica , Vetrugno Roberto , D'Angelo Roberto , Zaniboni Anna , Montagna Pasquale 
Published: July 2007

AbstractWe describe a 33‐year‐old man who presented with lip and tongue nibbling and bleeding during sleep. Videopolysomnography revealed myoclonic jerks involving the masticatory and facial muscles recurring mainly during NREM sleep. There was no tonic EMG masticatory activity typical of bruxism. EMG analysis demonstrated the recruitment of V‐ to VII innervated muscles and, in half of the episodes, also the sternocleidomastoideus. Our patient had sleep‐related faciomandibular myoclonus (SFMM) with spontaneous jerks of oromasticatory and cervical muscles, occurring only during sleep. Tooth grinding, temporomandibular joint pain, abnormal tooth mobility, tooth wear, and other dental problems were clinically absent. We propose that, on the basis of the clinical and EMG features, SFMM may be considered a distinct disorder and different from sleep bruxism. © 2007 Movement Disorder Society
Isolated tongue tremor after removal of cerebellar pilocytic astrocytoma: Functional analysis with SPECT study
Authors: Kim Suk Jae , Lee Won Yong , Kim Byoung Joon , Kim Ji‐Youn , Hong Seung Bong , Tae Woo Suk , Seo Dae‐Won 
Published: July 2007

AbstractTongue tremor is a rare form of focal tremor. Even though the dysfunction of the dentato‐rubro‐olivary circular pathway has been proposed as its mechanism, the origin of the rhythmic activities remains unknown. We experienced a case of isolated tongue tremor after the removal of a cerebellar pilocytic astrocytoma. To localize the activated structures corresponding to the isolated tongue tremor, we analyzed subtracted ictal SPECT coregistered to MRI (SISCOM). SISCOM demonstrated multiregional hyperperfusion including the Guillain‐Mollaret triangular and extratriangular structures. The activation of the inferior olive and red nucleus and accompanying extratriangular structures might be related to the isolated tongue tremor. © 2007 Movement Disorder Society
Medullary infarcts may cause ipsilateral masseter reflex abnormalities
Authors: Thömke Frank , Marx Jürgen J. , Cruccu Giorgio , Stoeter Peter , Hopf Hanns C. 
Published: July 2007

AbstractThere is a suprasegmental influence on the masseter reflex (MassR) in animals, which is mediated via the fifth nerve spinal nucleus (5SpN). Corresponding data in humans are lacking. Out of 268 prospectively recruited patients with clinical signs of acute brainstem infarctions, we identified 38 with magnetic resonance imaging (MRI)–documented unilateral infarcts caudal to the levels of the fifth nerve motor and main sensory nuclei. All had biplanar T2‐ and echo planar diffusion‐weighted MRI and MassR testing. Five patients (13%) had ipsilateral MassR abnormalities. In all, the infarcts involved the region of the 5SpN. Patients with medullary infarcts involving the region of the 5SpN may thus have ipsilateral MassR abnormalities. This possibly represents an interruption of an excitatory projection mediated via the 5SpN to masseter motoneurons in the fifth nerve motor nucleus. MassR abnormalities with medullary lesions restrict the topodiagnostic value of the MassR. Muscle Nerve, 2007
Lateral femoral cutaneous neuropathy and its surgical treatment: A report of 167 cases
Authors: Benezis Igor , Boutaud Benoit , Leclerc Jerome , Fabre Thierry , Durandeau Alain 
Published: July 2007

AbstractSurgical treatment of lateral femoral cutaneous neuropathy (LFCN) is performed only after failure of conservative management. We reexamined 167 cases (7 bilateral) of LFCN of various etiologies (idiopathic, abdominal surgery, iliac crest bone grafting, trauma, and total hip arthroplasty) operated on between 1987 and 2003. Average follow‐up was 98 months (20–212). The intervention was performed under local anesthesia in 139 cases (83%). Surgical release of the nerve was performed in 153 cases (92%) and transection in 14 cases (8%). Surgical treatment of LFCN led to improvement and patient satisfaction in 130 cases (78%). The results depended on several factors, especially the underlying etiology, duration of symptoms before intervention, and integrity of the nerve. Nerve release remains the first‐line surgical technique, improving painful symptoms in many cases while preserving sensation of the thigh. It can be performed under local anesthesia by an experienced surgeon. Muscle Nerve, 2007
Using prescription claims data for drugs available over‐the‐counter (OTC)
Authors: Yood Marianne Ulcickas , Campbell Ulka B. , Rothman Kenneth J. , Jick Susan S. , Lang Janet , Wells Karen E. , Jick Hershel , Johnson Christine C. 
Published: July 2007

AbstractPurposeMany pharmacoepidemiologic studies use automated prescription claims data to estimate the association between exposure and disease. One limitation of automated data, when studying drugs that are also available via retail, is that over‐the‐counter (OTC) exposure is missed. The purpose of this study is to quantify the effect of misclassification of OTC use in research that uses prescription claims data as the sole source of exposure information.MethodsWe conducted a sensitivity analysis in the context of studies of non‐steroidal anti‐inflammatory drugs (NSAIDs) and colorectal cancer. The following factors were widely varied to examine the impact on the validity of the effect estimate for NSAIDs and colorectal cancer: (1) the overall prevalence of NSAID exposure in the population, (2) the proportion of NSAID exposure due to OTC use (the prevalence of missed NSAID exposure in studies of prescription claims), and (3) the true risk ratio (RRtrue). We graphed the RR that would be observed (RRobserved) as a function of overall prevalence of NSAID use and the prevalence of NSAID use that is OTC exposure.ResultsWe found that when the true RR ranges from 0.25 to 0.75, missing OTC drug exposure is not a large source of bias in those situations in which the overall prevalence of drug use is relatively low (less than 35%) and the proportion of drug use that is OTC exposure is as high as 80%.ConclusionResults from our sensitivity analysis indicate that, in many circumstances, prescription claims data can give valid estimates of association even though some of the drugs are available OTC. Copyright © 2007 John Wiley & Sons, Ltd.
Predictive factors of self‐medicated drug use among the Spanish adult population
Authors: Carrasco‐Garrido P. , Jiménez‐García R. , Barrera V. Hernández , Gil de Miguel A. 
Published: July 2007

AbstractPurposeOur study aimed at describing the prevalence of self‐medicated drug use among the Spanish adult population and to identify the predictive factors of such self‐medication in Spain.MethodsDescriptive, cross‐sectional study covering the Spanish adult population, using data drawn from the 2003 Spanish National Health Survey (ENSS). A total of 19 514 subjects were analysed. The independent variables were socio‐demographic and health‐related, and the dependent variable was self‐medicated drug use. Using logistic multivariate regression models we have estimated the independent effect of each of these variables on the self‐medicated consumption.ResultsThe 18.1% of all Spaniards indulge in self‐medication. Our study shows that 45% of all cold and influenza medication and 39.4% of analgesics consumed by the population involve self‐medication. It should be stressed here that 17.7% of persons who took antibiotics did so in the form of self‐medication. The variables that were independently and significantly associated with a greater probability of self‐medicated consumption were: sex; lower age; higher educational level; consumption of alcohol; smoking habit; use of alternative medical products; absence of chronic disease and a positive perception of health.ConclusionsIn Spain, the prevalence of self‐medicated drug use is higher in women than men. In our population, the influence of unhealthy lifestyles, such as alcohol and tobacco consumption, is related to a higher likelihood of self‐medication. Copyright © 2007 John Wiley & Sons, Ltd.
Elaboration of open‐cell microcellular nanocomposites
Authors: Lépine O. , Birot M. , Deleuze H. 
Published: July 2007

AbstractStable water‐in‐oil high internal phase emulsions, containing styrene and divinylbenzene monomers and exfoliated montmorillonite, were prepared and polymerized to obtain nanocomposite microcellular materials. The porous structure was investigated by scanning electron microscopy, mercury intrusion porosimetry, and nitrogen adsorption/desorption analyses. The exfoliation of clay was investigated by X‐ray diffraction and transmission electron microscopy analyses. The presence of inorganic filler did not modify the microcellular structure of the composite, while the use of modified clay significantly enhanced its mechanical properties. No influence on the thermal degradation was noted, except for materials with high clay content that tended to deteriorate at lower temperature than the other materials. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 4193–4203, 2007