bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-Dose α-Tocopherol Therapy Does Not Affect HDL Subfractions in Patients with Coronary Artery Disease on Statin Therapy
Authors: Singh Uma , Otvos James , Dasgupta Amitava , de Lemos James A , Devaraj Sridevi , Jialal Ishwarlal 
Published: January 2007

Abstract
Background: Subfractions of HDL, particularly large HDL (HDL2), are inversely correlated with the severity of coronary artery disease (CAD). α-Tocopherol (AT) is the main lipid-soluble antioxidant in plasma. Results of a previous small study (n = 44) suggested that either a combination of an antioxidant cocktail [800 IU/day 2R,4′R,8′R-(RRR)-AT plus 1 g vitamin C, 25 mg β-carotene, and 100 μg selenium] or individual antioxidant vitamins combined with simvastatin–niacin (S-N) therapy attenuated the protective increase in HDL2 seen with S-N alone. Few data are available on the effect of AT therapy alone on HDL subfractions, which we addressed in this study.
Methods: In a prospective placebo-controlled study, we randomized 127 patients with stable CAD to receive high-dose RRR-AT (1200 IU/day for 2 years) or placebo. HDL subfractions (small, medium, and large HDL particles) were analyzed by nuclear magnetic resonance spectroscopy.
Results: AT concentrations significantly increased in the AT arm but not with placebo. No differences were noted between AT and placebo groups in concentrations of total cholesterol, triglyceride, LDL-cholesterol, or HDL-cholesterol. AT therapy did not affect total, small, medium, or large HDL particles compared with baseline or placebo. Furthermore, serum apolipoprotein A1 concentrations did not change after 2 years AT therapy as compared with baseline.
Conclusions: High-dose AT therapy administered for a 2-year period does not negatively affect either HDL subfractions or apolipoprotein A1 in patients with CAD on statin therapy. Thus the negative interaction previously proposed between antioxidant cocktail and statin therapy cannot be attributed to AT.
Tethered Epidermal Growth Factor Provides a Survival Advantage to Mesenchymal Stem Cells
Authors: Fan Vivian H. , Au Ada , Tamama Kenichi , Littrell Romie , Richardson Llewellyn B. , Wright John W. , Wells Alan , Griffith Linda G. 
Published: January 2007

AbstractMSC can act as a pluripotent source of reparative cells during injury and therefore have great potential in regenerative medicine and tissue engineering. However, the response of MSC to many growth factors and cytokines is unknown. Many envisioned applications of MSC, such as treating large defects in bone, involve in vivo implantation of MSC attached to a scaffold, a process that creates an acute inflammatory environment that may be hostile to MSC survival. Here, we investigated cellular responses of MSC on a biomaterial surface covalently modified with epidermal growth factor (EGF). We found that surface-tethered EGF promotes both cell spreading and survival more strongly than saturating concentrations of soluble EGF. By sustaining mitogen-activated protein kinase kinase-extracellular-regulated kinase signaling, tethered EGF increases the contact of MSC with an otherwise moderately adhesive synthetic polymer and confers resistance to cell death induced by the proinflammatory cytokine, Fas ligand. We concluded that tethered EGF may offer a protective advantage to MSC in vivo during acute inflammatory reactions to tissue engineering scaffolds. The tethered EGF-modified polymers described here could be used together with structural materials to construct MSC scaffolds for the treatment of hard-tissue lesions, such as large bony defects.Disclosure of potential conflicts of interest is found at the end of this article.
Cyclooxygenase-2-Derived Prostaglandin E2 Protects Mouse Embryonic Stem Cells from Apoptosis
Authors: Liou Jun-Yang , Ellent David P. , Lee Sang , Goldsby Jennifer , Ko Bor-Sheng , Matijevic Nena , Huang Jaou-Chen , Wu Kenneth K. 
Published: January 2007

Abstract
Little is known about prostaglandin synthesis and function in embryonic stem cells. We postulated that mouse embryonic stem (mES) cells possess enzymes to synthesize protective prostaglandins. Compared with differentiated adult cells, mES cells were less susceptible to H2O2-induced apoptosis. However, their apoptosis was enhanced by indomethacin or SC-236, a selective inhibitor of cyclooxygenase (COX)-2. Analysis of COX pathway enzymes by Western blotting revealed expression of COX-2 and cytosolic and microsomal prostaglandin E2 (PGE2) synthases. COX-1 and prostacyclin (PGI2) synthases were undetectable. mES cells produced PGE2 but not PGI2. Importantly, PGE2 rescued mES cells from apoptosis. To elucidate the signaling mechanism by which PGE2 inhibits apoptosis, we analyzed E-type prostaglandin (EP) receptors by Western blots. All EP isoforms were detected except EP4. Butaprost, a specific EP2 agonist, rescued mES cells from apoptosis, whereas sulprostone, an EP1/EP3 agonist, had no effect, suggesting selective interaction of PGE2 with EP2. The antiapoptotic effect of PGE2 was abrogated by Ly-294002 or wortmannin but not H-89 or a specific inhibitor of protein kinase A, suggesting signaling via phosphatidylinositol-3 kinase (PI-3K). Akt was constitutively active in mES cells, which were inhibited by indomethacin and rescued by PGE2. The rescuing effect of PGE2 was abrogated by Ly-294002. These results indicate that mES cells constitutively express COX-2 and PGE synthases and produce PGE2, which confers resistance to apoptosis via EP2-mediated activation of PI-3K to the Akt pathway.
Disclosure of potential conflicts of interest is found at the end of this article.
Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography–tandem mass spectrometry
Authors: Ding Cun‐Gang , Zhou Zhen , Ge Qing‐Hua , Zhi Xiao‐Jin , Ma Li‐Li 
Published: January 2007

AbstractA method has been developed for the simultaneous quantification of metformin (I) and glipizide (II) in human plasma. It is based on high‐performance liquid chromatography with electrospray ionization tandem mass (LC‐ESI‐MS/MS) spectrometric detection in positive ionization mode. Phenformin (III) and gliclazide (IV) were used as internal standards for I and II, respectively. The MS/MS detection was performed in multiple reaction monitoring (MRM) mode. The precursor‐product ion combinations of m/z 130 → 71, 446 → 321, 206 → 60 and 324 → 127 were used to quantify I, II, III and IV, respectively. This method was validated in the concentration ranges of 0.02–4 µg/mL for I and 0.004–0.8 µg/mL for II. It was utilized to support a clinical pharmacokinetic study after single dose oral administration of a combination of I and II. Copyright © 2007 John Wiley & Sons, Ltd.
The determination of raloxifene in rat tissue using HPLC
Authors: Yang Zhaoyong , He Xiaobo , Zhang Yueqin 
Published: January 2007

AbstractWe report a rapid and reliable HPLC‐UV method for determination of raloxifene, a kind of selective estrogen receptor modulator (SERM), in rat tissue. Proteins were precipitated by adding 200 µL of acetonitrile and 50 µL of methanol to 100 µL of the tissue homogenates, following vortex mixing and centrifugation. Separation was carried out on a reversed‐phase C18 column (150 × 4.6 mm, 5 µm) with a mobile phase of acetonitrile:0.05 m ammonium acetate (pH 4.0 ± 0.1; 33:67, v/v) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 289 nm and the temperature of column was kept at 23°C, without interference from endogenous tissue compounds. The calibration curve was linear from 0.0125 to 10.0 µg/mL with correlation coefficient of over 0.994, while the limit of quantification was 0.008 µg/mL. The intra‐ and inter‐day coefficients of variation were less than 10% (RSD). The recovery of assay was between 95.8 and 104.5%. Furthermore, the method was used to measure the concentration of raloxifene in rat tissue after a simple oral dose. The highest level was observed in liver, lung, spleen, then heart and kidney. The lowest level was found in brain. These results suggest that raloxifene distributes rapidly and moderately into tissues such as liver, lung and spleen. Copyright © 2007 John Wiley & Sons, Ltd.
Rapid identification of Helicobacter pylori by capillary electrophoresis: an overview
Authors: Buszewski B. , Kłodzińska E. , Dahm H. , Różycki H. , Szeliga J. , Jackowski M. 
Published: January 2007

AbstractHelicobacter pylori can cause gastritis and peptic ulcers and is directly associated with the development of gastric cancer. There are many types of diagnostic methods used to identification H. pylori (invasive and non‐invasive), but these methods usually require time‐consuming and laborious procedures and therefore are not capable of fast diagnosis in cases of emergency. This contribution describes the new achievements, interdisciplinary significance and some future directions in the application of capillary electrophoresis for determination of H. pylori. Copyright © 2007 John Wiley & Sons, Ltd.
An improved high‐throughput liquid chromatographic/tandem mass spectrometric method for terbinafine quantification in human plasma, using automated liquid–liquid extraction based on 96‐well format plates
Authors: Dotsikas Yannis , Apostolou Constantinos , Kousoulos Constantinos , Tsatsou Georgia , Loukas Yannis L. 
Published: January 2007

AbstractA fully automated high‐throughput liquid chromatography/tandem mass spectrometry (LC‐MS/MS) method was developed for terbinafine quantification in human plasma. The plasma samples were treated by liquid–liquid extraction (LLE) in 2.2 mL 96‐deepwell plates. Terbinafine and the internal standard (IS) N‐methyl‐1‐naphthalenemethylamine were extracted from human plasma by LLE, using a mixture of methyl t‐butyl ether (MTBE)–hexane (70:30, v/v) as the organic solvent. All liquid transfer steps, including preparation of calibration standards and quality control samples, as well as the addition of the IS, were performed automatically by using robotic liquid handling workstations. After vortexing, centrifugation and freezing, the supernatant organic solvent was evaporated and reconstituted in a small volume of a reconstitution solution. Sample analysis was performed by reversed‐phase LC‐MS/MS, with positive ion electrospray ionization, using multiple reaction monitoring (MRM). The method had a very short sample preparation time and a chromatographic run time of 2.2 min. It was proved to have excellent sensitivity, specificity, accuracy as well as inter‐ and intraday precision for the quantification of terbinafine in human plasma. The calibration curve was linear for the range of concentrations 5.0–2000.0 ng/mL. The proposed method was applied to the rapid and reliable determination of terbinafine in a bioequivalence study after per os administration of 250 mg tablet formulations of terbinafine. Copyright © 2007 John Wiley & Sons, Ltd.
A solid‐phase extraction method for high‐performance liquid chromatographic determination of salvianolic acid B in rabbit plasma: application to pharmacokinetic study
Authors: Ma Yueming , Wang Tianming 
Published: January 2007

AbstractA sensitive solid‐phase extraction/high‐performance liquid chromatographic method with ultraviolet detection was established for the analysis of salvianolic acid B in rabbit plasma. The analyte was separated on a reversed‐phase column with trifluoroacetic acid–methanol–acetonitrile (70:10:20, v/v/v) as mobile phase at a flow rate of 1 mL/min, and ultraviolet detection at 315 nm. The calibration curve for salvianolic acid B was linear over the range 35–1400 µg/L with coefficients of correlation >0.999. The inter‐day and intra‐day precisions of analysis were <15%, and assay accuracy ranged from 95.3 to 109.1%. This method is suitable for determining salvianolic acid B in plasma and thus investigating the pharmacokinetics of salvianolic acid B. Copyright © 2007 John Wiley & Sons, Ltd.
Semi‐micro column high‐performance liquid chromatography with UV detection for quantification of aspirin and salicylic acid and its application to patients' sera administered with low‐dose enteric‐coated aspirin
Authors: Ohwaki Yuichi , Yamane Tomoko , Ishimatsu Takashi , Wada Mitsuhiro , Nakashima Kenichiro 
Published: January 2007

AbstractA simultaneous determination of aspirin (ASA) and its metabolite, salicylic acid (SA), in human serum by a semi‐micro column HPLC‐UV was developed. A relatively small size of serum sample (100 µL) containing ASA and SA was cleaned up by a simple solid phase extraction. A good separation of ASA and SA could be achieved within 25 min using a semi‐micro ODS column with an eluent of MeOH/0.7 mm phosphoric acid solution (pH 2.5) = 50:50 (v/v). The calibration curves for ASA and SA showed good linearity (r = 0.999) with the detection limits 114 and 38 ng/mL at a signal‐to‐noise ratio of 3, respectively. ASA and SA in patients' sera administered with low‐dose enteric‐coated aspirin were determined, and the concentration ranges obtained for ASA and SA were 1.2–2.2 and 0.5–57.3 µg/mL, respectively. Copyright © 2007 John Wiley & Sons, Ltd.
Development of a LC‐ESI/MS/MS assay for the quantification of vanillin using a simple off‐line dansyl chloride derivatization reaction to enhance signal intensity
Authors: Beaudry Francis , Ross Andréanne , Vachon Pascal 
Published: January 2007

AbstractVanillin is responsible for producing the familiar smell of vanilla. Vanillin has many similarities with other flavor phenolic compounds and could potentially show similar pharmacological activity. A previously published analytical method was adapted, developed and tested. Vanillin was extracted from rat plasma using protein precipitation with acetone. Prior to LC‐ESI/MS/MS analysis, an aliquot of the supernatant was used to proceed to the derivatization of vanillin and the internal standard with dansyl chloride to enhance signal intensity in positive electrospray mode. The chromatography was performed on a 100 × 2.1 mm C8 column and an isocratic mobile phase composed of 75:25 acetonitrile:0.5% formic acid in water with a flow rate fixed at 500 µL/min. A linear (weighted 1/concentration) relationship was used to perform the calibration over an analytical range of 10–10,000 ng/mL. The intra‐batch precision and accuracy at the limit of quantitation (10 ng/mL), medium (500 ng/mL) and high (10,000 ng/mL) concentrations were 10.7, 7.0 and 7.2% and 103.5, 108.0 and 100.1%, respectively. The observed recovery was greater than 87% and no significant ionization suppression or matrix effect was observed. This LC‐ESI/MS/MS method for the determination of vanillin in rat plasma provided results within generally accepted criteria used for bioanalytical assay. Copyright © 2007 John Wiley & Sons, Ltd.
Direct detection method of oligosaccharides by high‐performance liquid chromatography with charged aerosol detection
Authors: Inagaki Shinsuke , Min Jun Zhe , Toyo'oka Toshimasa 
Published: January 2007

AbstractA simple and rapid detection method of oligosaccharides using high‐performance liquid chromatography with a charged aerosol detection (HPLC‐CAD) was studied. The direct detection of a sialylglycopeptide (SGP) derived from egg yolk was accomplished by HPLC‐CAD using an amido‐silica column, and its limit of detection was 0.40 pmol [signal‐to‐noise ratio (S/N) = 3]. The sensitivity of this method was lower than that of the fluorescence detection; however, the method showed approximately 5 times higher sensitivity than that using the conventional UV absorbance detection. Furthermore, this method was used for the analysis of the acid hydrolysis products of SGP. Monosialo‐ and asialo‐oligosaccharides as well as free sialic acid were detected without using fluorescent derivatization. These results indicate that the present method is a new tool for the analysis of oligosaccharides. Copyright © 2007 John Wiley & Sons, Ltd.
Development and validation of an RP‐HPLC‐UV method for the determination of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug, in rat plasma
Authors: Maltese Adriana , Maugeri Francesco , Ward Keith W. , Bucolo Claudio 
Published: January 2007

AbstractA simple and rapid high‐performance liquid chromatographic method was developed and validated for the analysis in rat plasma of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug. Liquid–liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C18 column using acetonitrile and water containing 1% triethylamine pH 3.5, adjusted with orthophosphoric acid (35.5:64.5 v/v) as mobile phase. The UV detector was set at 324 nm. The method proved to be linear (r2 > 0.99) and precise (RSD < 7%) over the concentration range 29–940 ng/mL, and was suitable for the support of pharmacokinetic studies in rats. Copyright © 2007 John Wiley & Sons, Ltd.
Determination of six bioactive components of Saussurea katochaete by capillary electrophoresis
Authors: Yue Mei‐E , Li Ya , Shi Yan‐Ping 
Published: January 2007

AbstractThe simultaneous determination of 3,4‐dimethoxy‐3′‐hydroxy propiophenone, 5‐hydroxy‐7‐methoxy coumarin, 7‐hydroxy coumarin, 3′,5′‐dimethoxy apigenin, apigenin and 4‐hydroxy cinnamic acid in the extract of S. katochaete has been investigated by capillary electrophoresis for the first time. The six active components were completely separated within 10 min in 20 mM Na2HPO4 buffer at pH 11.00 with 10% (v/v) methanol and detected at 214 nm. The applied voltage was 15 kV and the temperature was kept at 25°C. The effects of buffer pH, the concentration of Na2HPO4 and the concentration of methanol on the separation efficiency were studied systematically. The regression equations revealed good linear relationships (correlation coefficients were 0.9987–0.9998) between the peak area of each analyte and its concentration. The relative standard deviations (RSD) of migration time and peak areas were <1.63 and <4.03%, respectively. The application of this method for the separation and determination of the six bioactive components in S. katochaete was reported. The contents of the six analytes ranged from 0.023 to 0.131 mg/g and recoveries ranged from 94.7 to 104.8%. Copyright © 2007 John Wiley & Sons, Ltd.
Determination of hydroxysafflor yellow A in rat plasma and tissues by high‐performance liquid chromatography after oral administration of safflower extract or safflor yellow
Authors: Li Yi , Zhang Zhao‐yang , Zhang Jin‐lan 
Published: January 2007

AbstractA simple and reproducible HPLC method for quantification of hydroxysafflor yellow A (HSYA) in rat plasma and tissues after oral administration of safflower extract or safflor yellow (SY) was developed. Sample preparation was achieved by protein precipitation of plasma and tissue homogenates with three volumes of methanol. p‐Hydroxybenzaldehyde was used as the internal standard (IS). HSYA and IS were separated on a Hypersil BDS‐C18 column with a gradient elution system composed of acetonitrile and aqueous acetic acid. UV detection was used at 320 nm. The calibration curves were linear in all matrices (r2 > 0.999) in the concentration ranges 0.51–101.36 µg/mL for plasma, 12.27–2454.46 µg/g for intestines and 0.96–192.20 µg/g for lung. The intra‐day and inter‐day precision were all less than 12.5%, and the extract recovery was in the range 64.1–103.7% with RSD less than 15.6% for HSYA in all matrices. The method was used successfully to quantify HSYA in rat plasma and tissue samples to support a pharmacokinectic study. Copyright © 2007 John Wiley & Sons, Ltd.
Rapid quantification of lisinopril in human plasma by liquid chromatography/tandem mass spectrometry
Authors: Qin Weiwei , Zhang Zunjian , Tian Yuan , Xu Fengguo , Wang Na , Chen Yun 
Published: January 2007

AbstractAn assay based on protein precipitation and liquid chromatography/tandem mass spectrometry (LC‐MS/MS) has been developed and validated for the quantitative analysis of lisinopril in human plasma. After the addition of enalaprilat as internal standard (IS), plasma samples were prepared by one‐step protein precipitation using perchloric acid followed by an isocratic elution with 10 mm ammonium acetate buffer (pH adjusted to 5.0 with acetic acid)–methanol (70:30, v/v) on a Phenomenex Luna 5µC18 (2) column. Detection was performed on a triple‐quadrupole mass spectrometer utilizing an electrospray ionization (ESI) interface operating in positive ion and selected reaction monitoring (SRM) mode with the precursor to product ion transitions m/z 406→246 for lisinopril and m/z 349→206 for enalaprilat. Calibration curves of lisinopril in human plasma were linear (r = 0.9973−0.9998) over the concentration range 2–200 ng/mL with acceptable accuracy and precision. The limit of detection and lower limit of quantification in human plasma were 1 and 2 ng/mL, respectively. The validated LC‐MS/MS method has been successfully applied to a preliminary pharmacokinetic study of lisinopril in Chinese healthy male volunteers. Copyright © 2007 John Wiley & Sons, Ltd.
Characteristics of offenders referred to a medium secure NHS personality disorder service: the first five years
Authors: Milton John , Duggan Conor , McCarthy Lucy , Costley‐White Amanda , Mason Lauren 
Published: January 2007

AbstractBackground As new services for offenders with personality disorder emerge in the UK, there is interest in the methods of assessments and characteristics of patients admitted.Aim To evaluate use of selection criteria for admission to a dedicated personality disorder service within medium secure hospital provision in the UK, and to test for features that discriminate between those admitted and those rejected.Method A structured multidisciplinary assessment was administered to offenders referred to a new personality disorder service in a medium secure psychiatric unit. The evaluation included self‐report and observer ratings across a range of domains including categorical axis I disorders and axis II disorders, dimensional assessment of personality and ratings of intellect and risk.Results Sixty‐eight male offenders were assessed, of whom 47 were offered admission. No differences were found between those accepted or rejected on demographic, offence and most clinical characteristics. Axis I comorbidity was high in both groups (over 90%). There was no indication of staff overriding exclusion criteria but men who were accepted had greater extraversion and conscientiousness on the NEO‐FFI.Conclusion Staff screening men for suitability for admission to this new specialist medium security hospital unit stayed true to given exclusion criteria in their decision not to offer a bed. Characteristics with face value as qualities necessary for engagement in treatments appeared to influence positive choices. Next steps will be to test the validity of choice with outcome studies. Copyright © 2007 John Wiley & Sons, Ltd.