bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbe tree metabolite interactions in the soil - phyllosphere continuum of poplar tree: when microbes rewire poplar root exudate and metabolome
Authors: Fracchia F. , Guinet F. , Engle N.L. , Tschaplinski T.J. , Veneault-Fourrey C. , Deveau A. 
Published: March 2024

ABSTRACTTrees are associated with a broad range of microorganisms colonising the diverse tissues of their host. However, the early dynamics of the assembly of the microbiota from the root to shoot axis and how it is linked to root exudates and metabolite contents of tissues remain unclear.Here, we characterized how fungal and bacterial communities are altering root exudates as well as root and shoot metabolomes in parallel with their establishment in poplar cuttings (Populus tremula x tremuloidesclone T89) over 30 days of growth. Sterile poplar cuttings were planted in natural or gamma-irradiated soils. Bulk and rhizospheric soils, root and shoot tissues were collected from day 1 to day 30 to track the dynamic changes of fungal and bacterial communities in the different habitats by DNA metabarcoding. Root exudates and root and shoot metabolites were analysed in parallel by gas chromatography-mass spectrometry.Our study reveals that microbial colonization triggered rapid and substantial alterations in both the composition and quantity of root exudates, with over 70 metabolites exclusively identified in remarkably high abundances in the absence of microorganisms. Noteworthy among these were lipid-related metabolites and defence compounds. The microbial colonization of both roots and shoots exhibited a similar dynamic response, initially involving saprophytic microorganisms and later transitioning to endophytes and symbionts. Key constituents of the shoot microbiota were also discernible at earlier time points in the rhizosphere and roots, indicating that the soil constituted a primary source for shoot microbiota.Furthermore, the microbial colonization of belowground and aerial compartments induced a reconfiguration of plant metabolism. Specifically, microbial colonization predominantly instigated alterations in primary metabolism in roots, while in shoots, it primarily influenced defence metabolism. This highlighted the profound impact of microbial interactions on metabolic pathways of plants, shedding light on the intricate interplay between plants and their associated microbial communities.
Cell-state dependent regulation of PPARγsignaling by ZBTB9 in adipocytes
Authors: Xu Xuan , Charrier Alyssa , Congrove Sunny , Buchner David A. 
Published: March 2024

AbstractAdipocytes play a critical role in metabolic homeostasis. Peroxisome proliferator-activated receptor-γ(PPARγ) is a nuclear hormone receptor that is a master regulator of adipocyte differentiation and function. ZBTB9 was predicted to interact with PPARγbased on large-scale protein interaction experiments. In addition, GWAS studies in the type 2 diabetes (T2D) Knowledge Portal revealed associations between Zbtb9and both BMI and T2D risk. Here we show that ZBTB9 positively regulates PPARγactivity in mature adipocytes. Surprisingly Zbtb9knockdown (KD) also increased adipogenesis in 3T3-L1 cells and human preadipocytes.E2Factivity was increased and E2F downstream target genes were upregulated inZbtb9-KD preadipocytes. Accordingly, RB phosphorylation, which regulates E2F activity, was enhanced inZbtb9-KD preadipocytes. Critically, an E2F1 inhibitor blocked the effects ofZbtb9deficiency on adipogenic gene expression and lipid accumulation. Collectively, these results demonstrate thatZbtb9inhibits adipogenesis as a negative regulator ofPpargexpression via altered RB-E2F1 signaling. Our findings reveal complex cell-state dependent roles of ZBTB9 in adipocytes, identifying a new molecule that regulates adipogenesis and adipocyte biology as both a positive and negative regulator of PPARγsignaling depending on the cellular context, and thus may be important in the pathogenesis and treatment of obesity and T2D.
Balancing donor health and plasma collection: a systematic review of the impact of plasmapheresis frequency
Authors: D’aes Tine , van den Hurk Katja , Schroyens Natalie , Mikkelsen Susan , Severijns Pieter , De Buck Emmy , O’Leary Peter , Tiberghien Pierre , Compernolle Veerle , Erikstrup Christian , Van Remoortel Hans 
Published: March 2024

AbstractMost plasma used for manufacturing plasma-derived medicinal products (PDMPs) such as albumin, immunoglobulin (Ig), and clotting factors is obtained from source plasma collected via plasmapheresis, the majority of which is contributed by the United States (US). While the demand for PDMPs continues to rise, it remains unclear whether high-frequency plasmapheresis, such as the twice-weekly plasma donation allowed in the US, may have any (long-term) adverse health effects on the donor.To investigate the frequency at which plasma can be donated without harm to the donor, the current systematic review explores the impact of plasma donation frequency on cardiovascular health, protein depletion, and adverse events in healthy plasma donors. We asked the following research question: What is the impact of plasmapheresis frequency (Intervention) on the safety or health (Outcome) of healthy donors (Population)?Six databases (PubMed, Embase, Web of Science, CINAHL, the Cochrane Library, and Transfusion Evidence Library), two clinical trial registries (ICTRP andclinicaltrials.gov), and the PROSPERO database were searched. Four observational and two experimental studies were included, and one ongoing randomized controlled trial was identified. The results showed that very high-frequency donation (twice per week) may result in a clinically relevant decrease in ferritin and bring IgG levels below the EDQM-defined lower threshold of 6 g/l. However, the evidence is of low to very low certainty, and solid conclusions are hindered by thehealthy donor effectand methodological limitations of the included studies. In order to determine a safe threshold donation frequency that minimizes any possible harmful effect on the donor, more high-quality prospective cohort studies and experimental studies are thus needed. In the meantime, we argue for a precautionary approach and suggest that a sustainable and stable plasma supply may better rely on a large number of voluntary donors donating at a lower frequency (up to two donations per month), rather than on a small number of donors donating at a high frequency.
The expected polygenic risk score (ePRS) framework: an equitable metric for quantifying polygenetic risk via modeling of ancestral makeup
Authors: Huang Yu-Jyun , Kurniansyah Nuzulul , Goodman Matthew O , Spitzer Brian W , Wang Jiongming , Stilp Adrienne , Laurie Cecelia , de Vries Paul S , Chen Han , Min Yuan-I , Sims Mario , Peloso Gina M , Guo Xiuqing , Bis Joshua C , Brody Jennifer A , Raffield Laura M , Smith Jennifer A , Zhao Wei , Rotter Jerome I , Rich Stephen S , Redline Susan , Fornage Myriam , Kaplan Robert , Franceschini Nora , Levy Daniel , Morrison Alanna C , Boerwinkle Eric , Smith Nicholas L , Kooperberg Charles , Psaty Bruce M , Zöllner Sebastian ,   the Trans-Omics in Precision Medicine Consortium, Sofer Tamar 
Published: March 2024

AbstractPolygenic risk scores (PRSs) depend on genetic ancestry due to differences in allele frequencies between ancestral populations. This leads to implementation challenges in diverse populations. We propose a framework to calibrate PRS based on ancestral makeup. We define a metric called “expected PRS” (ePRS), the expected value of a PRS based on one’s global or local admixture patterns. We further define the “residual PRS” (rPRS), measuring the deviation of the PRS from the ePRS. Simulation studies confirm that it suffices to adjust for ePRS to obtain nearly unbiased estimates of the PRS-outcome association without further adjusting for PCs. Using the TOPMed dataset, the estimated effect size of the rPRS adjusting for the ePRS is similar to the estimated effect of the PRS adjusting for genetic PCs. Similarly, we applied the ePRS framework to six cardiovascular-related traits in the All of Us dataset, and the results are consistent with those from the TOPMed analysis. The ePRS framework can protect from population stratification in association analysis and provide an equitable strategy to quantify genetic risk across diverse populations.
Voxel-wise hemispheric Amyloid Asymmetry and its association with cerebral metabolism and grey matter density in cognitively normal older adults
Authors: Jayaprakash Hunsica J. , Mizuno Akiko , Snitz Beth E. , Cohen Ann D. , Klunk Willian E. , Aizenstein Howard J. , Karim Helmet T. 
Published: March 2024

AbstractIntroductionAlzheimer’s disease (AD) is a neurodegenerative disorder characterized by changes in beta amyloid (Aß) and tau as well as changes in cerebral glucose metabolism and gray matter volume. This has been categorized as three distinct stages of amyloid, tau, and neurodegeneration. Past studies have shown asymmetric Aβ accumulation and its association with asymmetric cerebral metabolism in preclinical AD. We analyzed data to replicate these findings and extend them to associations with gray matter volume and cognitive function.MethodsWe recruited 93 (mean age = 76.4±6.1 years) cognitively normal adults who underwent magnetic resonance imaging (MRI) and positron emission tomography (PET) with Pittsburgh compound B (PiB) and Fluorodeoxyglucose (FDG) tracers (to estimate Aβ and glucose metabolism, respectively). We conducted voxel-wise paired t-test on PiB (left vs. right hemispheres) to identify regions that differ in Aβ between the left and right cortex. We identified whether these regions showed asymmetry in FDG and gray matter volume using paired t-tests on each region. We then conducted correlations between asymmetry indices for each region that had significant asymmetry in PiB, FDG, and gray matter volume. We ran a group regression analysis on cognitive functions.ResultsWe found 26 regions that had significant rightward asymmetry in PiB including prefrontal cortex, temporal cortex, insula, parahippocampus, caudate, and putamen. All these regions showed significant gray matter rightward asymmetry, and most of these regions showed significant FDG asymmetry except the caudate, orbital cortex, medial frontal gyrus, and superior temporal gyrus. Only in the superior frontal gyrus, we found that greater rightward asymmetry in PiB was associated with greater rightward asymmetry in FDG,r(82) =0.38,p<0.005 (FDR corrected) – no other regions showed significant Aß asymmetry correlation with either FDG or gray matter volume asymmetry. We found that greater rightward FDG asymmetry in the superior frontal gyrus was associated with greater visuospatial processing scores in our cognitive domain group regression analysis.DiscussionAD has previously been modeled in three-stages: however, our results indicate that cerebral glucose metabolism may be dynamic throughout the disease progression and may serve as a compensatory pathway for maintaining cognitive functioning.
Opto-controlled C9orf72 poly-PR forms anisotropic condensates causative of TDP-43 pathology in the nucleus
Authors: Hodgson Rachel E , Rayment Jessica , Huang Wan-Ping , Avila Anna Sanchez , Shelkovnikova Tatyana A 
Published: March 2024

AbstractProteinaceous inclusions formed byC9orf72derived dipeptide-repeat (DPR) proteins are a histopathological hallmark in ~50% of familial amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD) cases. However DPR aggregation/inclusion formation could not be efficiently recapitulated in cell models for four out of five DPRs. In this study, using optogenetics, we achieved chemical-free poly-PR condensation/aggregation in cultured cells, with spatial and temporal control. Strikingly, nuclear poly-PR condensates had anisotropic, hollow-centre appearance, resembling anisosomes formed by aberrant TDP-43 species, and their growth was limited by RNA. These condensates induced abnormal TDP-43 granulation in the nucleus without the activation of stress response. Cytoplasmic poly-PR aggregates that formed under prolonged light stimulation were more persistent than its nuclear condensates, selectively sequestered TDP-43 in a demixed state and surrounded spontaneous stress granules. Our data suggest that poly-PR anisotropic condensation in the nucleus, causative of nuclear TDP-43 dysfunction, may constitute an early pathological event in C9-ALS/FTD. Anisosome-type condensates may represent a more common cellular pathology in neurodegeneration than previously thought.Highlights-Optogenetics can be used to modelC9orf72DPR condensation in cultured cells.-Opto-PR forms hollow nuclear condensates, and RNA limits their growth by fusion.-Opto-PR condensation leads to stress-independent TDP-43 pathology in the nucleus.-Cytoplasmic poly-PR assemblies are persistent and selectively sequester TDP-43.Graphical abstract
Hairpin-locker mediated CRISPR/Cas tandem system for ultrasensitive detection of DNA without pre-amplification
Authors: Deng Fei , Sang Rui , Li Yi , Yang Biyao , Zhai Xiwen , Xue Ruier , Zhang Chengchen , Deng Wei , Goldys Ewa M. 
Published: March 2024

AbstractAchieving ultra-sensitive detection of DNA is of paramount importance in the field of molecular analytics. Conventional amplification technologies such as polymerase chain reaction (PCR) currently play a leading role in ultrasensitive DNA detection. However, amplicon contamination common in these techniques may lead to false positives. To date, CRISPR-associated nucleases (type V & VI) with their programmable cleavage have been utilised for sensitive detection of unamplified nucleic acids in complex real samples. Nevertheless, without additional amplification strategies, the pM range sensitivity of such CRISPR/Cas sensors is not sufficient for clinical applications. Here, we established a hairpin-locker (H-locker) mediated Cas12-Cas13 tandem biosensing system (Cas12-13 tandem-sensor) for ultrasensitive detection of DNA targets. Without the need for any additional amplification reaction or device, this system is capable of detecting DNA at a notable 1 aM level (<1 copy/uL) sensitivity. In addition, the system was able to distinguish cancer mutations in colorectal cancer (CRC) mice. This is a significant advance for CRISPR/Cas biosensing technology offering simple, highly sensitive, and user-friendly diagnostics for next-generation nucleic acid detection.
Modulating Cas13atrans-cleavage by double strand RNA: Application to the development of an autocatalytic sensor
Authors: Deng Fei , Gulati Sneha , Sang Rui , Li Yi , Goldys Ewa M. 
Published: March 2024

AbstractCas13a-based diagnostic systems have been widely utilized for the detection of RNA targets. However, without preamplification such systems have sensitivity in the picomolar range only. Here, we found that double strand RNA (dsRNA) over 20nt is able to effectively activate thetrans-cleavage activity of Cas13a RNP, while the cleavage rates of dsRNA by activated Cas13a RNP are very low. In addition, specially designed small circular RNA constructs (Cir-mediators comprising a 20nt dsRNA trigger with a 5nt ssRNA linker) have limited ability to activate Cas13a RNP, but this activation is restored once the circular structures are cleaved and become linear. Based on this new method to controltrans-cleavage activity of Cas13 RNP, we developed a Cas13a autocatalytic biosensing system assisted by Cir-mediators, which allows one target RNA to activate numerous Cas13a RNPs. With this approach we show ultrasensitive detection of 1aM of synthetic RNA targets without preamplification within 15min. The sensor was successfully applied to monitor miRNA-21 concentration in clinical plasma samples in colorectal cancer. This investigation yields novel insights into the properties of Cas13a RNPs, and the Cir-mediator-based autosensor introduces a novel method for detecting RNA targets with exceptional sensitivity.HighlightsdsRNA is able to trigger activation of Cas13a RNP.Activated Cas13a RNP do not cleave dsRNA.Cir-mediator induces low levels of Cas13a RNP activation.Cir-mediator based Cas13a auto-catalysis biosensor can detect 1aM RNA targets.
Prophages block cell surface receptors to ensure survival of their viral progeny
Authors: Taylor Véronique L. , Shah Megha , Patel Pramalkumar H. , Yusuf Ahmed , Burk Cayla M. , Gitai Zemer , Davidson Alan R. , Koch Matthias D. , Maxwell Karen L. 
Published: March 2024

SummaryIn microbial communities, viruses compete for host cells to infect, and thus evolved diverse ways to inhibit their competitors. One mechanism is Superinfection exclusion (Sie), whereby a virus that has established an infection prevents a secondary infection. We identified aPseudomonasprophage Sie protein that alters pilus dynamics through the pilus assembly chaperone, PilZ. This protein, known as Zip for PilZ interactingprotein, does not abrogate pilus activity, but fine tunes it, providing strong phage resistance without a fitness cost. This tuning is modulated through quorum sensing, which coordinates Zip production in concert with bacterial cell density to ensure maximal protection when bacterial populations are at the highest risk of phage infection. Most notably, Zip activity prevents internalization and destruction of phage progeny. We refer to this as the “anti-Kronos effect” after the Greek god who devoured his own children and show that it is a conserved feature of diverse prophage-encoded Sie systems.
Measuring the patronage intentions among football fans: the role of corporate social responsibility, brand credibility and brand equity
Authors: Mansouri Hossein , Rasaee Rad Abdullah , Tsiotsou Rodoula H. , Md Husin Maizaitulaidawati 
Published: March 2024

PurposeThe study aims to identify critical factors that influence football fans’ support of their favorite team by examining the impact of social responsibility, brand credibility and team brand equity on patronage intentions of professional football teams.Design/methodology/approachAn online survey collected data from 331 football fans of the Persian Gulf Premier League (PGPL) in Iran. The data were analyzed using partial least squares structural equation modeling (PLS-SEM).FindingsThe findings revealed that corporate social responsibility (CSR) is able to influence brand equity, brand credibility and patronage intentions. Also, brand equity and brand credibility were found to be positively related to patronage intentions. In addition to that, the findings show that brand equity and team credibility partially mediate the relationship between CSR and patronage intentions.Practical implicationsThe findings provide valuable insights to sports teams/club managers aiming to attract new fans and retain current ones by investing in CSR and enhancing brand credibility and equity. Strategies to integrate CSR into relationship marketing and brand management are outlined.Originality/valueThis study empirically highlights the critical role of adhering to CSR and the effects of brand credibility and equity in enhancing patronage intentions among football team fans.
The role of mindfulness skill and inclusive leadership in job performance among secondary teachers in Hong Kong
Authors: Hong Liang , Zainal Siti Rohaida Mohamed 
Published: March 2024

Purpose
Researcher agreed that job performance has a positive effect on productivity as well as an organisation’s efficiency. Thus, this study aims to investigate the impact of mindfulness skill, inclusive leadership (IL), employee work engagement and self-compassion on the overall job performance of secondary school teachers in Hong Kong. It then evaluates the mediating effect of employee work engagement between the relationships of mindfulness skill, IL and job performance, as well as the moderate effect of self-compassion between the relationships of mindfulness skill, IL and employee work engagement.

Design/methodology/approach
The sample comprised 263 teachers working from three secondary schools in Sha Tin, Hong Kong. The data was then analysed using Smart PLS version 4.0.9.

Findings
The results showed significant positive relationships between mindfulness skill and IL towards employee work engagement and between employee work engagement and job performance; meanwhile, there emerged a significant effect on the relationship between mindfulness skill and IL towards job performance. Furthermore, this research has confirmed that self-compassion did not moderate the relationship between mindfulness skill, IL and employee work engagement, but employee work engagement plays a mediating effect on the relationship between mindfulness skill, IL and job performance.

Originality/value
This research has helped to fill the literature gap by examining the mediating roles of employee work engagement and mediator role of self-compassion in the integrated relationship of multi-factor and job performance. Examining the mediating role of employee work engagement has helped to enhance the understanding of the underlying principle of the indirect influence of mindfulness skill, IL and job performance. The result of this research shows that self-compassion plays a vital role in influencing the employees’ work engagement. Hence, it is important that companies design human resource management policy that enables self-compassion to be used as a consideration psychological-related strategy when structing organisation or teams. It is also crucial for top management and policymakers to define and communicate the organisation’s operating principle, value and goals.
The influence of place attachment and emotional solidarity on residents’ involvement in tourism: perspectives from Orlando, Florida
Authors: Aleshinloye Kayode D. , Woosnam Kyle M. , Joo Dongoh 
Published: March 2024

PurposeUsing the Stimulus-Organism-Response (SOR) model as a theoretical guide, this study employed a conceptual model involving residents’ place attachment (S) to the destination in which they live and emotional solidarity with tourists (O) as precursors to their involvement in tourism (R). The purpose of this paper is threefold: To determine (1) whether residents’ place attachment explains their emotional solidarity with tourists, (2) if emotional solidarity is an effective predictor of residents’ involvement in tourism planning and development and (3) if emotional solidarity dimensions mediate the relationship between place attachment and involvement.Design/methodology/approachData were collected from 378 permanent resident heads of households living in, or adjacent to, central Orlando, using a self-administered survey with a census-guided systematic sampling method. Data were subjected to tests of normality and common method bias, followed by a two-step confirmatory factor analysis and structural equation modeling.FindingsSeven of the 11 proposed model hypotheses were supported, with moderate variances explained in each of the four outcome variables: welcoming nature (R2 = 19.3%), emotional closeness (R2 = 24.5%), sympathetic understanding (R2 = 39.4%) and involvement (R2 = 36.8%). Though both place identity and place dependence (as two dimensions of place attachment) were partial mediators, the former proved to be more pronounced.Originality/valueThis study employed non-economic measures—place attachment and emotional solidarity—in determining residents’ involvement in tourism within their community. Such an approach provides fresh insights into how such symbolic constructs can contribute to residents’ positive, actionable involvement in tourism. This research is one of the few that have incorporated emotional solidarity as a construct within the SOR model and the first to examine the indirect effects (through mediation) of emotional solidarity.