bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterobimetallic Ruthenium‐Cobalt Complexes Containing the Pentamethylcyclopentadienyl or Indenyl Ligand
Authors: Ng Sin Yee , Goh Lai Yoong , Koh Lip Lin , Leong Weng Kee , Tan Geok Kheng , Ye Suming , Zhu Yinghuai 
Published: December 2005

AbstractThe salt elimination reaction between NaCo(CO)4 and the ruthenium complexes LRu(diphos)Cl and [(Ind)Ru(CO)2Cl] afforded the Ru–Co bimetallic complexes [LRu(μ‐CO)2(μ‐diphos)Co(CO)2] [L = C5Me5 (Cp*), diphos = Ph2P(CH2)nPPh2, 3a: n = 1 , or 3b: n = 2; 3: L = C9H7 (Ind), diphos = Ph2PCH2PPh2 ] and [(Ind)Ru(CO)2Co(CO)4] (3d), respectively, in high yields. However, the same reaction with the monophosphane analogue, [(Ind)Ru(PPh3)2Cl], gave the heterobimetallic complex [(Ind)Ru(PPh3)(CO)(μ‐CO)Co(CO)3] (3e) in fair yield, together with redox‐initiated derivatives Ru(PPh3)2(CO)3 (4e) and [(Ind)Co(CO)(PPh3)] (6e). A similar redox process in the reaction of the dppf analogue, [(Ind)Ru(dppf)Cl] (dppf = 1,1′‐diphenylphosphanylferrocene) gave [Ru(dppf)(CO)3] (4f) and [(Ind)Co(CO)2] (6f), together with a complex salt [(Ind)Ru(dppf)(CO)][Co(CO4)] [5f·Co(CO)4]. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
A Hetero Diels–Alder Concerted vs. Aldol Stepwise Mechanism in the Cyclization of Silyloxyazadienes with Aldehydes: A Theoretical Study
Authors: Bongini Alessandro , Panunzio Mauro 
Published: December 2005

AbstractThe Diels–Alder reaction of 3‐silyloxy‐2‐azadiene with formaldehyde has been studied at the B3LYP/6‐31G* level of theory and the role played by a Lewis acid evaluated. It is shown that the reaction is preferred when the Lewis acid coordinates to the aldehyde oxygen. This coordination allows for a concerted as well as a stepwise mechanism for the cyclization. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Synthesis of Spiro Carba‐Sugars by Ring‐Closing Metathesis
Authors: González‐Bello Concepción , Castedo Luis , Cañada Francisco Javier 
Published: December 2005

AbstractSix conformationally restricted carba‐sugars have been synthesized by ring‐closing metathesis of a readily available diallyl keto derivative of (–)‐quinic acid. The rich functionality of the resulting spiro ketone was exploited for the diastereoselective synthesis of various spiro carba‐sugars: four of them polyhydroxylated and two aminopolyhydroxylated. The results of the testing of these compounds against various commercially available glycosidases (amyloglicosidase, α‐ and β‐galactosidase, α‐ and β‐ glucosidase, α‐mannosidase, trehalase and neuraminidase) are provided. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Neuroblastoma cell death in response to docosahexaenoic acid: Sensitization to chemotherapy and arsenic‐induced oxidative stress
Authors: Lindskog Magnus , Gleissman Helena , Ponthan Frida , Castro Juan , Kogner Per , Johnsen John Inge 
Published: December 2005

AbstractDocosahexaenoic acid (DHA) is an ω‐3 polyunsaturated fatty acid vital for the developing nervous system and significantly decreased in neuroblastoma cells compared to nontransformed nervous tissue. We investigated whether supplementation of DHA affects the susceptibility of neuroblastoma cells to oxidative stress generated endogenously and in response to cytotoxic therapy. DHA, but not the monounsaturated oleic acid (OA), induced dose‐ and time‐dependent neuroblastoma cell death. DHA supplementation was associated with depolarization of the mitochondrial membrane potential, production of reactive oxygen species (ROS) and accumulation of DNA in sub‐G1 phase of the cell cycle. The antioxidant, vitamin E, inhibited mitochondrial depolarization and subsequent cell death induced by DHA, whereas, the mitochondrial pore inhibitor, cyclosporin A, partly inhibited DHA‐induced neuroblastoma cell death. Depletion of glutathione by L‐buthionine‐sulfoximine significantly enhanced the cytotoxic effects of DHA. Nontransformed fibroblasts were not substantially affected by DHA. DHA, but not OA, significantly enhanced the cytotoxicity of cisplatin, doxorubicin and irinotecan both in chemosensitive and in multidrug‐resistant neuroblastoma cells. DHA potently sensitized neuroblastoma cells to a clinically relevant concentration (1 μM) of arsenic trioxide (As2O3) and enhanced the effect of the nonsteroidal antiinflammatory drug (NSAID), diclofenac. These findings provide experimental evidence that the ω‐3 fatty acid, DHA, is cytotoxic to drug‐resistant neuroblastoma. The potent action of DHA with arsenic trioxide, NSAID and chemotherapeutic agents suggests clinical testing of this therapeutic concept in children with neuroblastoma. © 2005 Wiley‐Liss, Inc.
Body size and composition and the risk of gastric and oesophageal adenocarcinoma
Authors: MacInnis Robert J. , English Dallas R. , Hopper John L. , Giles Graham G. 
Published: December 2005

AbstractAlthough evidence has been mounting that obesity may be related to the increased incidence of oesophageal and gastric cardia malignancies, these reports (mainly case‐control studies) have relied on imperfect measures of obesity such as body mass index (BMI), and generally have not clearly distinguished between anatomical subsites within the oesophagus and stomach. In a prospective study of people aged 27–75 years, we directly measured fat mass and fat‐free mass (using bioelectrical impedance analysis), height, weight and waist and hip circumferences. Among 41,295 people followed on average for 11.3 years, 30 cases with cancers in the gastric cardia or lower third of the oesophagus and 68 cases with noncardia gastric adenocarcinomas were ascertained via the population cancer registry. The risk of adenocarcinoma of the lower oesophagus and gastric cardia was positively associated with BMI with a hazards ratio (HR) and (95% confidence interval) for people with BMI ≥ 30 kg/m2 compared with those <25 kg/m2, of 3.7 (1.1–12.4), an HR per 10 cm increase in waist circumference of 1.46 (1.05–2.04), and a HR per 10 kg increase on fat‐free mass of 2.06 (1.15–3.69). Noncardia gastric adenocarcinoma showed little relationship with body size. We observed an increased risk of adenocarcinoma of the lower oesophagus and gastric cardia associated with increased BMI, central adiposity and the nonfat component of weight, but found no association with noncardia gastric adenocarcinoma. An increasing prevalence of obesity may be associated with the increasing incidence of gastro‐oesophageal cancer observed in many populations. © 2005 Wiley‐Liss, Inc.
Definition of an HPV18/45 cross‐reactive human T‐cell epitope after DNA immunisation of HLA‐A2/KB transgenic mice
Authors: McCarthy Corinna , Youde Sarah J. , Man Stephen 
Published: December 2005

AbstractAlthough human papillomavirus (HPV) types 16 and 18 are the most common types associated with cervical cancer worldwide, other related HPV types such as HPV 35, 45 and 58 have significant prevalence in geographically distinct populations. For development of global prophylactic and therapeutic vaccine strategies, it is important to study immune responses against these viruses and to define the degree of cross‐reactivity between related HPV types. To investigate the potential for T cell cross‐reactivity after vaccination, HLA‐A2/Kb transgenic mice were immunised with DNA plasmid constructs containing HPV18 and 45 E6 and E7. Splenocytes from immunised mice were tested in direct ELIspot assays against overlapping pools of HPV 18 peptides. Immunisation with either HPV18 or HPV45 E6 DNA produced dominant T cell responses against an epitope (KCIDFYSRI) that was shared between HPV18 and HPV45. This peptide was shown to bind to HLA‐A*0201 but not Db or Kb molecules on the cell surface. Furthermore this peptide was shown to be immunogenic in vitro to human T cells from 2 out of 3 HLA‐A2+ healthy donors. Collectively, these results demonstrate that HPV 18 and 45 E6 DNA vaccines are immunogenic in mice and demonstrate that cross‐reactive T cell responses against closely related HPV types can be induced in vivo. The use of the HLA‐A2/Kb transgenic mice allowed definition of an HLA‐A*0201 binding peptide epitope that would have been rejected on the basis of predicted major histocompatibility complex binding affinity. © 2005 Wiley‐Liss, Inc.
Comparative genomic hybridization analysis on male breast cancer
Authors: Rudlowski Christian , Schulten Hans‐Jürgen , Golas Mariola Monika , Sander Bjoern , Barwing Roland , Palandt Jens‐Ekkehard , Schlehe Bettina , Lindenfelser Rüdiger , Moll Roland , Liersch Torsten , Schumpelick Volker , Gunawan Bastian , Füzesi László 
Published: December 2005

AbstractThe spectrum of genetic alterations in primary male breast cancer is not well established. We analyzed chromosomal imbalances in 39 tumor samples from primary male breast cancer by comparative genomic hybridization (CGH) and correlated CGH findings with clinicopathological factors. Chromosomal gains were most frequent at 1q (46%), 8q (46%), 16p (36%), 17q (36%), Xq (28%), 20q (26%) and Xp (18%). Losses were most commonly observed at 8p (36%), 16q (28%), 13q (28%), 6q (18%), 11q (18%) and 22q (18%). Gains at 16p, 20q and Xq and losses at 13q correlated significantly with higher degree of cytogenetic complexity. Significant associations with clinicopathological factors were observed for +8q and −16q with larger tumor size and −16q with lower proliferative activity and lower grade of malignancy. A comparison with reported CGH data from female breast cancer showed a similar pattern of chromosomal imbalances, including +1q, −8p, +8q, −13q, +16p, −16q, +17q and +20q. Our results indicate that male breast cancer shares a common pattern of imbalances with female breast cancer, suggesting that similar genetic events may underlie the development and progression of male and female breast cancer. © 2005 Wiley‐Liss, Inc.
Age of diagnosis of colorectal cancer in HNPCC patients is more complex than that predicted by R72P polymorphism in TP53
Authors: Talseth Bente A. , Meldrum Cliff , Suchy Janina , Kurzawski Grzegroz , Lubinski Jan , Scott Rodney J. 
Published: December 2005

AbstractHereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominantly inherited cancer syndrome associated with germline mutations in DNA mismatch repair (MMR) genes. Recently a polymorphism at codon 72 (R72P) in the tumour suppressor gene TP53 has been implicated in the age of disease onset in HNPCC. In this report we have studied a large cohort of HNPCC patients to assess the impact of this polymorphism on disease expression and age of diagnosis of colorectal cancer (CRC). DNA samples from 218 HNPCC mutation positive patients from Australia and Poland were genotyped for the arginine to proline change at codon 72 in the TP53 gene. The association between the polymorphism and disease characteristics (mutation status, disease expression and age of diagnosis of CRC) was tested using Pearson's Chi‐square and Kaplan–Meier survival analysis. Our study of Australian and Polish HNPCC patients does not provide evidence for an association between the Arg/Pro (GC) genotype of the R72P polymorphism and age of diagnosis of CRC. The R72P polymorphism was examined in HNPCC patients and found to be not associated with disease development in either the Australian or Polish populations. When gene mutation status (hMLH1 or hMSH2) was included in the analysis some evidence of an affect was observed. The genotyping revealed in the Australian population that the R72P polymorphism was under‐represented in the hMSH2 group whereas it was over‐represented in the Polish hMSH2 group. A similar trend was observed for hMLH1 in both groups but was not significant. Age of diagnosis of CRC in HNPCC patients is therefore more complex than that predicted by the R72P TP53 polymorphism alone, suggesting an inter‐relationship with other genetic and/or environmental factors. © 2005 Wiley‐Liss, Inc.
Association of the ARLTS1 Cys148Arg variant with familial breast cancer risk
Authors: Frank Bernd , Hemminki Kari , Meindl Alfons , Wappenschmidt Barbara , Klaes Rüdiger , Schmutzler Rita K. , Untch Michael , Bugert Peter , Bartram Claus R. , Burwinkel Barbara 
Published: December 2005

AbstractRecently, ARLTS1 (ADP‐ribosylation factor‐like tumor suppressor gene 1) has been identified as a tumor suppressor gene, playing a major role in apoptotic signaling. The ARLTS1 Trp149Stop mutation has been shown to predispose to general familial cancer and high‐risk familial breast cancer (BC), provoking the attenuation of apoptotic function. We studied the impact of the ARLTS1 Pro131Leu and Cys148Arg variants on high‐risk familial and familial BC risk, investigating 482 familial BC cases (including 305 high‐risk cases) and 530 control individuals. Unlike ARLTS1 Pro131Leu, Cys148Arg revealed a significant association with an increased risk of high‐risk familial BC (odds ratio (OR) = 1.47, 95% confidence interval (95% CI) = 1.04–2.06, p = 0.03) in a dose‐dependent manner (ptrend = 0.007). The genotype distribution of Cys148Arg in familial cases was similar, indicating significance as well (OR = 1.48, 95% CI = 1.10–1.99, p = 0.009; ptrend = 0.003). On the basis of the small number of 46 cases, we additionally showed an association between the Trp149Stop mutation and an increased risk of bilateral BC (OR = 4.11, 95% CI = 1.27–13.31, p = 0.011). © 2005 Wiley‐Liss, Inc.
Environmental life cycle assessment of a pre‐fragmented high explosive grenade
Authors: Hochschorner Elisabeth , Hägvall Joakim , Finnveden Göran , Griffing Evan , Overcash Michael 
Published: December 2005

AbstractOrganisations today face increasing environmental constraints, e.g. in the form of legal and customer requirements; the defence sector is no exception. There is a need to evaluate and limit environmental effects of defence activities and materiel. In this study we used quantitative Life Cycle Assessment (LCA) and a method for simplified LCA (the Material, Energy, Chemicals and Others (MECO) method) to assess the environmental impacts of a grenade. The aims of the study are to identify aspects in the grenade's life‐cycle that have the largest environmental impact, suggest improvement possibilities, make a comparison between different approaches for waste management of munitions, and to perform a demonstrative case for the application of LCA to munitions. Significant environmental aspects of the grenade's life‐cycle include use of metals, use of fossil fuels, and detonation outdoors. The study shows that an LCA can be used to analyse environmental impacts from munitions. The simplified LCA gave information that is complementary to the quantitative LCA. Copyright © 2005 Society of Chemical Industry
Power Doppler assessment of overall disease activity in patients with rheumatoid arthritis
Authors: Kiris Adem , Ozgocmen Salih , Kocakoc Ercan , Ardicoglu Ozge 
Published: December 2005

AbstractPurpose.To examine synovial vascularity and flow patterns in hand and wrist joints—metacarpophalangeal (MCP) joints and ulnar stiloid (USTL) regions—of patients with rheumatoid arthritis (RA) using power Doppler sonography (PDUS) and spectral Doppler analysis and to assess the accuracy of PDUS in detecting overall disease activity in RA patients.MethodsTwo hundred forty MCP joints and 48 USTL regions in 24 RA patients were examined. Patients were categorized into 2 groups—active and inactive—according to the American College of Rheumatology remission criteria. Resistance indexes (RIs) were measured.ResultsFlow signals were detected in 50 MCP joints (in 13 patients) and 24 USTL regions (in 16 patients) and spectral analysis was performed in 46 MCP joints (12 patients) and 23 USTL regions (16 patients). The sensitivity and specificity of PDUS in detecting disease activity in RA were 92% and 40%, respectively. There was a negative correlation between flow signal number and RI, with higher scores of flow signals corresponding to lower RIs.ConclusionPDUS appears to be a reliable method for assessing inflammatory activity in rheumatoid synovium. © 2006 Wiley Periodicals, Inc. J Clin Ultrasound 34:5–11, 2006
Incidence and clinical significance of spontaneous intrahepatic portosystemic venous shunts detected by sonography in adults without potential cause
Authors: Lin Zu‐Yau , Chen Shinn‐Cherng , Hsieh Ming‐Yen , Wang Chu‐Wen , Chuang Wan‐Long , Wang Liang‐Yen 
Published: December 2005

AbstractPurposeB‐mode and color Doppler sonography were used to assess the incidence and clinical significance of spontaneous intrahepatic portosystemic venous shunt in adults without detectable cause.MethodsA total of 25,579 adults without potential cause of intrahepatic portosystemic venous shunt were screened using B‐mode sonography. Suspicion of shunt was confirmed by color Doppler sonography. Patients with shunt were followed up with sonography at 6‐ to 12‐month intervals and received dietary education to prevent the ingestion of extra‐large amounts of protein. During each sonography session, the patients were asked to report any experience of consciousness disturbance.ResultsSix patients (0.0235%) showed evidence of shunt on color Doppler sonography. Direct communication between a right portal branch and the inferior vena cava was found in 3 patients; a small aneurysm connecting a peripheral portal branch to the hepatic vein was found in the remaining 3 patients. Five patients were followed up (range, 12–60 months) and showed no change in the size of the shunt. None of the patients with shunt reported an experience of consciousness disturbance.ConclusionsThe incidence of spontaneous intrahepatic portosystemic venous shunt is extremely low in asymptomatic adult patients. Follow‐up and dietary control seen to be suitable for their management. © 2006 Wiley Periodicals, Inc. J Clin Ultrasound 34:22–26, 2006
The influence of athletic activity on the plantar fascia in healthy young adults
Authors: Uzel Murat , Cetinus Ercan , Ekerbicer H. Cetin , Karaoguz Ahmet 
Published: December 2005

AbstractPurposeComplaints deriving from the plantar fascia are relatively common in athletes. This study aimed to investigate the changes of thickness of plantar fascia via sonography in healthy young adults with different levels of activity.Patients and MethodsOne hundred ten adults with normal body mass index were separated into three groups according to activity level: sedentary (group 1, n = 50), athletic activity less than 7 hours per week (group 2, n = 30), and athletic activity 7 or more hours per week (group 3, n = 30). The thicknesses of the plantar fascia at origin and at a point 5 mm distal to origin were measured via sonography.ResultsThe mean values of the thickness of the proximal plantar fascia (PFp) and the distal plantar fascia (PFd) in group 1 were similar to those of groups 2 and 3 (p > 0.05). The mean values of PFp and PFd were significantly higher in men than in women (p < 0.05). The mean values of PFp and PFd were similar in left and right feet (p > 0.05). There were moderate positive correlations between PFp and weight, height, and body mass index but no correlation between PFp and amount of athletic activity.ConclusionsThe thickness of the plantar fascia at origin did not change with athletic activity at the amateur level. © 2006 Wiley Periodicals, Inc. J Clin Ultrasound 34:17–21, 2006
In vivo electrogene transfer of interleukin‐12 inhibits tumor growth and lymph node and lung metastases in mouse mammary carcinomas
Authors: Shibata Masa‐Aki , Ito Yuko , Morimoto Junji , Kusakabe Ken , Yoshinaka Ryoji , Otsuki Yoshinori 
Published: December 2005

AbstractBackgroundHuman breast cancer metastasizes mainly to lymph nodes, lungs, liver, and bone; in the majority of cases, it is the development of metastases which leads to death. In order to suppress mammary cancer metastasis, we applied in vivo electrogene transfer (non‐viral method) as a means of interleukin‐12 (IL‐12) gene therapy on highly metastatic murine mammary cancer model.MethodsMetastatic mammary tumors induced by inoculation in BALB/c female mice were treated by intratumoral injections of either a plasmid vector containing IL‐12 or empty vector and then subjected to in vivo electrogene transfer once a week for 8 weeks.ResultsTreatment with IL‐12 resulted in elevation of both IL‐12 and IFNγ levels in mammary tumors and in serum and intratumoral levels of CD4 and CD8 proteins were also increased. Tumor volumes and lymphatic and pulmonary metastases were significantly reduced. The histopathological changes induced by IL‐12 characteristically included marked inflammation, increased apoptosis, decreased DNA synthesis, peripheral influx of significantly greater numbers of active macrophages, and reduced blood microvessel density, and apoptotic vascular endothelial cells were frequently seen. Western blotting showed decreases in VEGFR‐3 of tumors exposed to IL‐12 gene therapy. In adjuvant immunofluorescence studies, the CD31‐positive endothelial cells of microvessels showed decreased VEGFR‐3 expression in IL‐12‐treated tumors. However, apparent alterations in VEGFR‐3 expression of podoplanin‐positive lymphatic endothelial cells were not observed in IL‐12‐treated tumors. Although recombinant IL‐12 did not inhibit tubular formation of human umbilical vein endothelial cells in a Matrigel assay, recombinant IFNγ did completely suppress the tubular formation.ConclusionsIn vivo electrogene transfer of IL‐12 exerts strong anti‐tumorigenic and anti‐metastatic effects likely due to T‐cell‐mediated immune responses as well as anti‐angiogenic action. Copyright © 2005 John Wiley & Sons, Ltd.
Repeat hepatectomy for recurrent colorectal liver metastases
Authors: Pessaux Patrick , Lermite Emilie , Brehant Olivier , Tuech Jean‐Jacques , Lorimier Gérard , Arnaud Jean‐Pierre 
Published: December 2005

AbstractPurposeLiver resection represents the best and potentially curative treatment for metastatic colorectal cancer (MCC) to the liver. After resection, however, most patients develop recurrent disease, often isolated to the liver. The aim of this study was to determine the value of repeat liver resection for recurrent MCC and to analyze the factors that can predict survival.Patients and MethodsFrom January 1992 to October 2002, 42 patients from a group of 168 patients resected for MCC were submitted to 55 repeat hepatectomies (42 second, 11 third, and 2 fourth hepatectomies). Records were retrospectively reviewed. The primary tumor was carcinoma of the colon in 26 patients and carcinoma of the rectum in 16 patients. Liver metastases were synchronous in 24 patients (57.1%).ResultsThere were 25 men and 17 women with the mean age of 63.5 years (range: 34–80). There was no intraoperative or postoperative mortality. The morbidity rates were 9.5%, 14.3%, and 18.2% (P = 0.6) respectively after a first, second, or third hepatectomies. No patients needed reoperation. Operative duration was longer after a second or third hepatectomie than after a first hepatectomie without difference for operative bleeding. Overall 5‐year survivals were 33%, 21%, and 36% respectively after a first, second, or third hepatectomies. Factors of prognostic value on univariate analysis included serum carcinoembryonic antigen levels (P = 0.01) during the first hepatectomy, the presence of extrahepatic disease (P = 0.05) and tumor size larger than 5 cm (P = 0.04) during the second hepatectomie.ConclusionsRepeat hepatectomies can provide long‐term survival rates similar to those of first hepatectomies. J. Surg. Oncol. 2006;93:1–7. © 2005 Wiley‐Liss, Inc.