bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trisomy 12 in HESC leads to no selective in vivo growth advantage in teratomas, but induces an increased abundance of renal development
Authors: Gertow Karin , Cedervall Jessica , Unger Christian , Szöke Krisztina , Blennow Elisabeth , Imreh Marta P. , Ährlund‐Richter Lars 
Published: November 2006

AbstractThe aim of this investigation was to examine the impact of chromosome 12 amplification (tri‐12 cells) in human embryonic stem cells (HESC), following in vivo engraftment to an immunodeficient xeno‐model. For this we used sublines from the HESC line HS181, spontaneously exhibiting either low or high frequencies of tri‐12 cells. Fluorescent in situ hybridization (FISH) analysis revealed a random distribution of tri‐12 cells in the HS181 colonies in vitro. Similarly, the contribution of tri‐12 cells to the development of various tissues in teratomas in vivo seemed to be fully random with no particular preference regarding in vivo differentiation pathway of tri‐12 HS181 cells compared to HS181 cells with disomy 12 (di‐12 cells). On the other hand, following in vivo transplantation the ratio of tri‐12/di‐12 cells was significantly reduced (P < 0.001), indicating a negative selection for this trisomy in vivo. Moreover, injection of HS181 cultures containing tri‐12 cells resulted in a significantly increased abundance of areas compatible with renal formation (P < 0.001), relative teratomas derived from injection of di‐12 HS181 cells. However, such areas included no increased relative frequency of tri‐12 cells, suggesting indirect mechanism(s) for the increased abundance of renal development. The reasons for such developmental bias are unknown and warrant further investigation. J. Cell. Biochem. 100: 1518–1525, 2007. © 2006 Wiley‐Liss, Inc.
Animal models for chronic lymphocytic leukemia
Authors: Pekarsky Yuri , Zanesi Nicola , Aqeilan Rami I. , Croce Carlo M. 
Published: November 2006

AbstractB‐cell chronic lymphocytic leukemia (B‐CLL), the most common leukemia in the Western world, results from an expansion of a rare population of CD5+ mature B‐lymphocytes. Although clinical features and genomic abnormalities in B‐CLL have been studied in considerable detail, the molecular mechanisms underlying disease development has remained unclear until recently. In the last 4 years, several transgenic mouse models for B‐CLL were generated. Investigations of these mouse models revealed that deregulation of three pathways, Tcl1‐Akt pathway, TNF‐NF‐kB pathway, and Bcl2‐mediated anti‐apoptotic pathway, result in the development of B‐CLL. While deregulation of TCL1 alone caused a B‐CLL phenotype in mice, overexpression of Bcl2 required aberrantly activated TNF‐NF‐kB pathway signaling to yield the disease phenotype. In this article, we present what has been learned from mice with B‐CLL phenotype and how these mouse models of B‐CLL were used to test therapeutic treatments for this common leukemia. J. Cell. Biochem. 100: 1109–1118, 2007. © 2006 Wiley‐Liss, Inc.
Network financial support and conflict as predictors of depressive symptoms among a highly disadvantaged population
Authors: Knowlton Amy R. , Latkin Carl A. 
Published: November 2006

AbstractThe study examined multiple dimensions of social support as predictors of depressive symptoms among a highly vulnerable population. Social network analysis was used to assess perceived and enacted dimensions of support (emotional, financial, instrumental), network conflict, closeness, and composition. Participants were 393 current and former injection drug users who were 72% ≤ poverty level, 96% African American, 39% HIV seropositive. At baseline, 37% had high depression scores (CES‐D ≥ 16). Adjusted logistic regression indicated that for every additional network member providing financial support, the odds of probable depression 1 year later decreased by 23%, and for every additional conflictive network tie the odds of depression increased by 57%. Findings suggest the greater importance to this population's psychological well‐being of received support specific to environmental demands, rather than support perceived potentially available. The findings suggest potential directions for intervention. © 2007 Wiley Periodicals, Inc.
Measuring homelessness and residential stability: The residential time‐line follow‐back inventory
Authors: Tsemberis Sam , McHugo Gregory , Williams Valerie , Hanrahan Patricia , Stefancic Ana 
Published: November 2006

AbstractReliable and valid longitudinal residential histories are needed to assess interventions to reduce homelessness and increase community tenure. This study examined the test‐retest reliability, sensitivity to change, and concurrent validity of the Residential Time‐Line Follow‐Back (TLFB) Inventory, a method used to record residential histories in the Collaborative Program to Prevent Homelessness (n = 1,381). The Residential TLFB Inventory yielded temporally stable aggregate measures of duration in residential categories, and it revealed significant differences in change over time when contrasting study groups. A comparison of agency and participant data at one site.
Psychological reactions to crime in Italy: 2002–2004
Authors: Amerio Piero , Roccato Michele 
Published: November 2006

AbstractWe performed a secondary analysis of the data collected by the Observatory of the North‐West (a mail panel representative of the Italian population over 18), describing the trends in the distribution of fear of crime (FC) and of concern about crime as a social problem (CC) in Italy between the end of 2002 and the beginning of 2004. After analyzing the main sociodemographic characteristics of the people who became more afraid and more concerned, we built two models for predicting such trends using three sets of independent variables, concerning: (a) the sociodemographic and victimization domains, (b) the psychosocial and political domains, and (c) the mass media domain.Main results were as follows: (a) even though correlated, FC and CC showed different distributions, because CC was less widespread than FC; (b) during the period in question, the spread of FC became broader, whereas the spread of CC was reduced; (c) in order to predict the trend in FC during the period in question, sociodemographic and victimization variables were sufficient; and (d) to predict the trend in CC it was useful to also use psychosocial and mass media variables. Implications, limitations, and future research directions are discussed. © 2007 Wiley Periodicals, Inc.
Contraction‐induced interleukin‐6 transcription in rat slow‐type muscle is partly dependent on calcineurin activation
Authors: Banzet Sébastien , Koulmann Nathalie , Sanchez Hervé , Serrurier Bernard , Peinnequin André , Alonso Antonia , Bigard Xavier 
Published: November 2006

AbstractThe present work aimed at determining whether interleukin‐6 (IL‐6) produced by skeletal muscle during exercise is related, at least partly, to calcineurin activity. Rats were treated with two specific calcineurin inhibitors, cyclosporin A (CsA) and FK506, or vehicle (Vhl); they were then subjected to exhaustive treadmill running. Modulatory Calcineurin‐Interacting Protein‐1 (MCIP‐1) mRNA levels, a reliable indicator of calcineurin activity, and IL‐6 mRNA levels were measured by real‐time RT‐PCR in soleus muscles, and IL‐6 protein concentration was measured in the plasma. Because low carbohydrates availability enhances IL‐6 transcription through p38 Mitogen Activated Protein Kinase (MAPK) pathway, muscle glycogen content and glycaemia were measured and p38 MAPK phosphorylation was determined in skeletal muscle by western blotting. As expected, exercise induced an increase in IL‐6 (P < 0.01) and MCIP‐1 mRNA (P < 0.01) in soleus muscle of Vhl rats, and enhanced p38 phosphorylation and plasmatic IL‐6 protein (P < 0.05). Calcineurin inhibition did not affect running time, glycemia or soleus glycogen content. CsA administration totally inhibited the exercise‐induced increase in MCIP‐1 mRNA (P < 0.01), blunted the IL‐6 gene transcription related to muscle activity, and suppressed the changes in IL‐6 protein in plasma. In addition to its inhibition of calcineurin activity, FK506 administration totally suppressed the exercise‐induced IL‐6 gene transcription, likely by an inhibition of p38 activation. Taken together, these results demonstrate that in addition to p38 MAPK, increased calcineurin activity is one of the signalling events involved in IL‐6 gene transcription. J. Cell. Physiol. 210: 596–601, 2007. © 2006 Wiley‐Liss, Inc.
The role of dynamin 3 in the testis
Authors: Vaid K.S. , Guttman J.A. , Babyak N. , Deng W. , McNiven M.A. , Mochizuki N. , Finlay B.B. , Vogl A.W. 
Published: November 2006

AbstractWe report here that dynamin 3 in the testis is associated with structures termed tubulobulbar complexes that internalize intact intercellular junctions during sperm release and turnover of the blood–testis barrier. The protein lies adjacent to an actin‐Arp2/3 network that cuffs the double plasma membrane tubular invagination at the core of each complex. To explore the possible relationship between dynamin 3 and nectin‐based adhesion junctions, we transiently transfected DsRed‐tagged dynamin 3 into MDCK cells stably transfected with eGFP‐tagged nectin 2, one of the adhesion molecules known to be expressed in Sertoli cells at adhesion junctions. Cells transfected with the dynamin 3 construct had less uniformly distributed nectin 2 at intercellular contacts when compared to control cells expressing only nectin 2 or transfected with the DsRed plasmid alone. Significantly, tubular extensions positive for nectin 2 were visible projecting into the cells from regions of intercellular contact. Our findings are consistent with the conclusion that dynamin 3 is involved with tubulobulbar morphogenesis. Dynamin 3 also occurs in concentrated deposits around the capitulum and striated columns in the connecting piece of sperm tails suggesting that the protein in these cells may function to stabilize the base of the tail or serve as a reservoir for use during or after fertilization. J. Cell. Physiol. 210: 644–654, 2007. © 2006 Wiley‐Liss, Inc.
Bcl‐2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells
Authors: Sheibani Nader , Scheef Elizabeth A. , DiMaio Terri A. , Wang Yongji , Kondo Shuji , Sorenson Christine M. 
Published: November 2006

AbstractBcl‐2 is the founding member of a family of proteins that influence apoptosis. During kidney development bcl‐2 not only acts as a survival factor, but may also impact cell adhesive mechanisms and by extension branching morphogenesis. The interrelationship between cell adhesion, migration and apoptosis, important during development, is poorly understood. Here we examined the impact lack of bcl‐2, an inhibitor of apoptosis, has on ureteric bud (UB) cell adhesion, migration, and branching morphogenesis. Bcl‐2 −/− UB cells demonstrated increased cell migration, increased cell invasion and decreased adhesion to vitronectin and fibronectin compared with wild‐type cells. Bcl‐2 +/+ UB cells readily branched in collagen gel and Matrigel while bcl‐2 −/− UB cells did not undergo significant branching in either matrix. Re‐expression of bcl‐2 in bcl‐2 −/− UB cells restored their ability to undergo branching morphogenesis in Matrigel. Consistent with our in vitro data, we show that in the absence of bcl‐2, embryonic kidneys undergo decreased UB branching. We observed decreased numbers of UB branch points, UB branch tips and a decreased distance to the first UB branch point in the absence of bcl‐2. The alterations in bcl‐2 −/− UB cell adhesion and migration was also associated with a significant alteration in expression of a number of extracellular matrix proteins. Bcl‐2 −/− UB cells exhibited increased fibronectin expression and decreased thrombospondin‐1 and osteopontin expression. Taken together, these data suggest that bcl‐2 is required for the proper regulation of cell adhesive and migratory mechanisms, perhaps through modulation of the cellular microenvironment. J. Cell. Physiol. 210: 616–625, 2007. © 2006 Wiley‐Liss, Inc.
Connective tissue growth factor (CTGF/CCN2) is a downstream mediator for TGF‐β1‐induced extracellular matrix production in osteoblasts
Authors: Arnott J.A. , Nuglozeh E. , Rico M.C. , Arango‐Hisijara I. , Odgren P.R. , Safadi F.F. , Popoff S.N. 
Published: November 2006

AbstractConnective tissue growth factor (CTGF/CCN2) is a cysteine‐rich, extracellular matrix (ECM) protein that acts as an anabolic growth factor to regulate osteoblast differentiation and function. Recent studies have identified CTGF as a downstream effector of transforming growth factor‐β1 (TGF‐β1) for certain functions in specific cell types. In this study, we examined the role of CTGF as a downstream mediator of TGF‐β1‐induced ECM production and cell growth in osteoblasts. Using primary cultures, we demonstrated that TGF‐β1 is a potent inducer of CTGF expression in osteoblasts, and that this induction occurred at all stages of osteoblast differentiation from the proliferative through mineralization stages. TGF‐β1 treatment of osteoblasts increased the expression and synthesis of the ECM components, collagen and fibronectin. When CTGF‐specific siRNA was used to prevent TGF‐β1 induction of CTGF expression, it also inhibited collagen and fibronectin production, thereby demonstrating the requirement of CTGF for their up‐regulation. To examine the effects of TGF‐β1 on osteoblast cell growth, cultures were treated with TGF‐β1 during the proliferative stage. Cell number was significantly reduced and the cells exhibited a decrease in G1 cyclin expression, consistent with TGF‐β1‐induced cell‐cycle arrest. Cultures transfected with CTGF siRNA prior to TGF‐β1 treatment showed an even greater reduction in cell number, suggesting that TGF‐β1‐induced growth arrest is independent of CTGF in osteoblasts. Collectively, these data demonstrate for the first time that CTGF is an essential downstream mediator for TGF‐β1‐induced ECM production in osteoblasts, but these two growth factors function independently regarding their opposing effects on osteoblast proliferation. J. Cell. Physiol. 210: 843–852, 2007. © 2006 Wiley‐Liss, Inc.
Calcific myonecrosis and tenosynovitis: Sonographic findings with correlative imaging
Authors: Finlay Karen , Friedman Lawrence , Ainsworth Kelly 
Published: November 2006

AbstractWe report the sonographic appearance of a case of calcific myonecrosis and tenosynovitis with correlative plain radiography and CT findings. Calcific myonecrosis is a rare complication of previous trauma that presents as a tumor‐like calcified mass years after an initial injury, most commonly affecting the anterior compartment of the lower leg. Although the clinical, radiographic, and MRI features of calcific myonecrosis have been well described, to our knowledge the sonographic appearance has not been reported previously in the literature. © 2006 Wiley Periodicals, Inc. J Clin Ultrasound 2007
Monitoring of the heavy‐metal hyperaccumulation in vegetal tissues by X‐ray radiography and by femto‐second laser induced breakdown spectroscopy
Authors: Kaiser J. , Samek O. , Reale L. , Lis˘ka M. , Malina R. , Ritucci A. , Poma A. , Tucci A. , Flora F. , Lai A. , Mancini L. , Tromba G. , Zanini F. , Faenov A. , Pikuz T. , Cinque G. 
Published: November 2006

AbstractThis article reports on the utilization of X‐ray microradiography and laser induced breakdown spectroscopy (LIBS) techniques for investigation of the metal accumulation in different part of leaf samples. The potential of the LIBS‐analysis for finding the proper plant species for phytoremediation is compared with the results of microradiography measurements at the HERCULES source at ENEA, Rome (Italy) and X‐ray microradiography experiments at the ELETTRA Synchrotron, Trieste (Italy). Microsc. Res. Tech., 2007. © 2006 Wiley‐Liss, Inc.
A new inducible adenoviral expression system that responds to inflammatory stimuli in vivo
Authors: Cai Gang , Nie Xiaomeng , Guo Pin'e , Guan Zheng , Zhang Jun , Shen Qian 
Published: November 2006

AbstractBackgroundGene transfer using inducible promoters, which control expression of transgenic proteins in response to physiological conditions, may have significant advantages. In this study, we tried to achieve an inducible adenoviral expression system for physiologically responsive gene therapy of autoimmune or inflammatory diseases.MethodsA luciferase reporter vector with a hybrid promoter containing the human IL‐1β enhancer region (−3690 to − 2720) and the human CIITA promoter IV (−399 to + 2) was constructed. A replication‐deficient adenovirus was engineered with luciferase controlled by the IL1β/CIITApIV promoter (Ad‐IL1β/CIITApIV‐Luc). The reporter vector or adenovirus was transfected to C57Bl/6 myeloid dendritic cells (DCs), RAW264.7, and Hep G2 to study the in vitro characteristics of this hybrid promoter. An inflammation model was prepared by injecting lipopolysaccharide (LPS) into Balb/c mice intraperitoneally (i.p.), and infected with Ad‐IL1β/CIITApIV‐Luc or Ad‐CMV‐Luc to study the in vivo characteristics of the IL1β/CIITApIV promoter.ResultsThe IL1β/CIITApIV hybrid promoter has pronounced promoter activity, broad‐range responsiveness to cytokines or LPS, and can be rechallenged after first induction. In the inflammation model, IL1β/CIITApIV could drive hepatic luciferase expression increasedly rapidly after LPS challenge and in a LPS dose‐dependent manner.ConclusionsUsing the IL1β/CIITApIV hybrid promoter in gene transfer vectors may make it possible to produce transgenic proteins in vivo in direct relationship with the intensity and duration of an individual's status. By providing endogenously controlled production of transgenic proteins, this approach might limit the severity of autoimmune or inflammatory response without interfering with the beneficial components of host defense and immunity. Copyright © 2006 John Wiley & Sons, Ltd.
Human herpesvirus 8 genoprevalence in populations at disparate risks of Kaposi's sarcoma
Authors: Kumar Navdeep , McLean Ken , Inoue Naoki , Moles David R. , Scully Crispian , Porter Stephen R. , Gee Teo Chong 
Published: November 2006

AbstractThe prevalence of human herpesvirus 8 (HHV‐8) in populations at different risks of developing Kaposi's sarcoma (KS) was assessed using a protocol involving immunomagnetic fractionation of CD45+ blood cells prior to detection of the HHV‐8 genome by nested PCR. Preliminary studies using blood of eight gay men infected by human immunodeficiency virus‐1 (HIV‐1) revealed that, for the detection of HHV‐8 DNA derived from open reading frame (ORF) 26 of the HHV‐8 genome, this protocol provided substantially higher rates (100%) compared to one involving red blood cell (RBC) lysis (0%) and to another requiring double density gradient centrifugation (DDGC) of leukocytes (13%). When the CD45+ fractionation protocol was applied to samples from 103 other HIV‐1‐infected patients (the vast majority of whom were gay men) and 100 blood donors, the ORF 26 DNA detection rates obtained were 37% and 8%, respectively. When DNA from the variable region 1 of ORF K1 was additionally amplified from samples of the blood donors, a detection rate of 9% was achieved. This rate was highly concordant with the ORF 26 DNA detection rate. Furthermore, the ORF K1 sequences were predominantly unique, assignable to genotypes A1, A4, and C3. When assays for anti‐HHV‐8 and anti‐herpes simplex viruses (HSV) 1 and 2 were applied, significant concordances between HHV‐8 DNA detection rates and those relating to anti‐HHV‐8 and to anti‐HSV 1 and 2 were more frequently observed for HIV‐1‐infected patients than for blood donors. The higher‐than‐expected HHV‐8 genoprevalence rate in blood donors requires further confirmation in view of its implications for post‐transfusion HHV‐8 transmission. J. Med. Virol. 79:52–59, 2007. © 2006 Wiley‐Liss, Inc.
Molecular epidemiology of norovirus gastroenteritis outbreaks in North Carolina, United States: 1995–2000
Authors: Tseng F.C. , Leon J.S. , MacCormack J.N. , Maillard J.‐M. , Moe C.L. 
Published: November 2006

AbstractNoroviruses (NoVs) are the most common cause of acute non‐bacterial gastroenteritis outbreaks in the US. We investigated 16 gastroenteritis outbreaks in North Carolina (NC), from 1995 to 2000, to further characterize the epidemiology of NoV using RT‐PCR on stool and ELISA on sera. NoV were identified in 14 outbreaks by RT‐PCR. Sequence analyses of the amplicons indicated the outbreak strains belonged to the following clusters: five GII/4, three GI/3, one GI/4, one GII/2, one GII/5, one GII/7, and one GII/13 (prototype strain). We detected NoV in stool samples from one outbreak but could not determine its specific cluster within the GII genogroup based on polymerase sequence analysis. The five GII/4 strains were classified as the “95/96 US common strain” and occurred throughout the 5‐year period. In contrast to national trends, the majority (86%) of NoV outbreaks identified in North Carolina were foodborne. Of the 12 food‐related NoV outbreaks, we were able to document transmission by food handlers in two outbreaks. Person‐to‐person transmission from primary cases was suggested in three outbreaks. Our results indicate that NoVs are important agents of viral gastroenteritis outbreaks in NC. J. Med. Virol. 79:84–91, 2007. © 2006 Wiley‐Liss, Inc.
Human T‐cell lymphotropic virus types I and II (HTLV‐I and ‐II) infection among seroindeterminate cases in Argentina
Authors: Berini Carolina A. , Eirin Maria E. , Pando Maria A. , Biglione Mirna M. 
Published: November 2006

AbstractHuman T‐cell lymphotropic virus (HTLV) seroindeterminate cases have been reported among blood donors (BD) and in at‐risk populations worldwide, including Argentina. The objective of the present work was to study the presence of HTLV‐I/II infection and its association to specific Western blot (WB) patterns among healthy BD and at‐risk populations in Argentina. We analyzed 83 HTLV‐I/II seroindeterminate WB cases diagnosed among BD (n = 49) and in different at‐risk populations (n = 34) for human retroviruses infections. Multiple indeterminate WB patterns were observed. Out of the total, 13.2% (11/83) of the cases were found to be HTLV‐I/II positive by nested‐PCR (n‐PCR), including 13.2% (11/83) HTLV‐I and 2.4% (2/83) presenting HTLV‐I and ‐II co‐infection. Most of their serological profiles showed reactivity to gag or env codified proteins. Two samples amplified only one of the six analyzed genes (1 HTLV‐I pol gene and 1 HTLV‐II tax gene). There was no association between the presence of Trypanosoma cruzi infection and an HTLV‐I/II indeterminate WB pattern (only 3 of the 83 samples were positive for T. cruzi antibodies). In conclusion, the majority of HTLV‐seroindeterminate WB donors lacked HTLV provirus and was thus considered uninfected. However, when seroreactivity to Env and Gag proteins are observed on the WB and especially in at‐risk populations, HTLV infection should be suspected; such individuals should be followed‐up and retested. J. Med. Virol. 79:69–73, 2007. © 2006 Wiley‐Liss, Inc.