bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear actin and DNA replication stress regulate the recruitment of human telomerase to telomeres
Authors: Harman Ashley , Kartawinata Melissa , Maroun Nohad M. , Nguyen Darren R. , Hughes William E. , Winardi Kevin , Cesare Anthony J. , Lamm Noa , Bryan Tracy M. 
Published: March 2024

SUMMARYThe recruitment of telomerase to telomeres is a tightly regulated process which is stimulated by replication stress and mediated by the DNA damage response regulatory kinase ATR. Here, we demonstrate that nuclear filamentous actin is important for telomerase recruitment under endogenous and replication stress conditions in immortal human cells. Inhibition of nuclear actin polymerization decreases the presence of telomerase at telomeres. This process is regulated by both ATR and mTOR kinases, and employs other regulators of actin structure and function, such as WASP, ARP2/3 and myosin. Nuclear filamentous actin serves as a site for telomerase recruitment, which is mediated by telomere tethering on actin fibres in response to replication stress, allowing telomerase to localize to telomeres containing stalled replication forks. Overall, these data demonstrate that, in human cells which express telomerase, telomeric replication stress triggers the recruitment of telomerase to telomeres via a nuclear actin network, enabling telomere length maintenance.
Liver regeneration by oval cells employing bistability of stemness-senescence, Hippo signaling, EMT-MET, and polyploidy circuit
Authors: Lazovska Marija , Salmina Kristine , Pjanova Dace , Gerashchenko Bogdan I. , Erenpreisa Jekaterina 
Published: March 2024

AbstractLiver hepatocytes possess remarkable regenerative capabilities, yet severe damage may compromise this process. Liver progenitor (“oval”) cells exhibit the potential to differentiate into both hepatocytes and cholangiocytes, making them promising candidates for cell therapy. However, their mechanisms in liver regeneration are not clear. Here, on rat liver oval stem-like epithelial cells (WB-F344) a wound healing assay was performed. The scratched near-confluent monolayers (70% area removed) underwent the G1-arrest, bi-nucleation at 10-12 hours post-wounding, starting movement of epithelial to mesenchymal transition (EMT) cell portion into the wounded areas. Nanog nuclear upregulation, fragmentation, and transition as granules into cytoplasm and around, along with p16Ink4anuclear intrusion from the cytoplasm, loss of epithelial markers, and YAP1/Hippo activation were seen near the wound edge. The replicative stress and proliferation boost followed, documented at 24 hours. Proliferation concluded at 40-48 hours, accomplished by reconstitution of epithelial tissue, the disappearance of Nanog granulation and p16Ink4areturn to the cytoplasm, releasing excess. This investigation reveals novel regulatory facets in liver regeneration by oval cells. It accentuates the stemness-senescence bistable switch regulated by reciprocal nucleo-cytoplasmic transitions of opposite regulators, coordinated with Hippo-pathway switch, replicative stress, and boost, along with ploidy, EMT-MET and paracrine secretome circuits - enabling successfully resolving the massive injury.Abstract FigureFig 1.Graphical abstract.Bistable nuclear-cytoplasmic switch between stemness and senescence regulators in the wound healing process by oval liver cells: (1-2) Priming phase: (1) at the wound edge, (2) in the wound; (3) Proliferative phase, wound closure. Nanog – green; p16INK4A – red, EMT cell - with blue nucleus.
BAX and BAK expression triggers multiple IRE1 signaling outputs under ER stress
Authors: Sepulveda Claudia , Milani Mateus , Morales Vania , Tamburinni Giovanni , Montes Nicolas , Hetz Claudio 
Published: March 2024

AbstractAdaptation to endoplasmic reticulum (ER) stress depends on the activation of the unfolded protein response (UPR) stress sensor inositol-requiring enzyme 1 alpha (IRE1). IRE1 is a central ER stress sensors, that signals through the activation of its RNase domain to catalyze the splicing the mRNA encoding the transcription factor X-box binding protein 1 (XBP1), resulting on the expression of a stable and active transcription factor termed XBP1s. The kinetics and amplitude of IRE1 signaling are regulated by different posttranslational modifications and the physical interaction of different factors. Early studies demonstrated that the expression of the proapoptotic proteins BAX and BAX enhance UPR signaling. However, the possible effects on the RNase activity were not defined. Here we provide preliminary evidence indicating that BAX and BAK deficiency increases the in 10 folds the threshold of ER stress to induce XBP1 mRNA splicing, and the upregulation of its target genes. In addition, the degradation of RIDD substrates was strongly reduced in BAX and BAK null cells. BAX and BAK double deficiency also attenuated the levels of IRE1 phosphorylation under mild ER stress. These results reinforce previous findings indicating that proapoptotic BAX and BAK have alternative functions at the ER regulating the UPR.
Dorsolateral septum GLP-1R neurons regulate feeding via lateral hypothalamic projections
Authors: Lu Yi , Wang Le , Luo Fang , Savani Rohan , Rossi Mark A. , Pang Zhiping P. 
Published: March 2024

AbstractObjectiveAlthough glucagon-like peptide 1 (GLP-1) is known to regulate feeding, the central mechanisms contributing to this function remain enigmatic. Here, we aim to test the role of neurons expressing GLP-1 receptors (GLP-1R) in the dorsolateral septum (dLS; dLSGLP-1R) and their downstream projections on food intake and determine the relationship with feeding regulation.MethodsUsing chemogenetic manipulations, we assessed how activation or inhibition of dLSGLP-1Rneurons affected food intake inGlp1r-ires-Cremice. Then, we used channelrhodopsin-assisted circuit mapping, chemogenetics, and electrophysiological recordings to identify and assess the role of the pathway from dLSGLP-1Rneurons to the lateral hypothalamic area (LHA) in regulating food intake.ResultsChemogenetic inhibition of dLSGLP-1Rneurons increases food intake. LHA is a major downstream target of dLSGLP-1Rneurons. The dLSGLP-1R→LHA projections are GABAergic, and chemogenetic inhibition of this pathway also promotes food intake. While chemogenetic activation of dLSGLP-1R→LHA projections modestly decreases food intake, optogenetic stimulation of the dLSGLP-1R→LHA projection terminals in the LHA rapidly suppressed feeding behavior. Finally, we demonstrate that the GLP-1R agonist, Exendin 4 enhances dLSGLP-1R→LHA GABA release.ConclusionsTogether, these results demonstrate that dLS-GLP-1R neurons and the inhibitory pathway to LHA can regulate feeding behavior, which might serve as a potential therapeutic target for the treatment of eating disorders or obesity.HighlightsChemogenetic inhibition of dLSGLP-1Rneurons boosts food intake in micedLSGLP-1Rneuron activation does not alter feeding, likely by collateral inhibitiondLSGLP-1Rneurons project to LHA and release GABAActivation of dLSGLP-1R→LHA axonal terminals suppresses food intakeGLP-1R agonism enhances dLSGLP-1R→LHA GABA release via a presynaptic mechanism
Chara canescenspart I: Oospore differentiation of parthenogenic and dioecious strains and salt-dependent oospore sizes
Authors: Holzhausen A. , Romanov R.E. , Schubert H. 
Published: March 2024

AbstractChara canescensLoisel. is one of two European species of the section Desvauxia R.D. Wood of the genusCharaL. Whereas most populations ofC. canescensreproduce parthenogenetically, a few sites with sexual reproducing populations are known. Studies of EuropeanC. canescensoospore morphology led to open questions about the taxonomic status. Here we investigated nearly 1000 oospores from 16 European populations originated from plant release, sediments, germination experiments and herbaria and two Asian populations resulting in a regional determination key for studied populations as well as important database implications regarding origin, oospore plant position and equipment used. The impact of salinisation on oospore morphology were tested by artificial salt levels. The longest but smallest oospores were formed at 3 PSU, whereas the widest at 0.1 PSU and the shortest at 5 PSU. Basal width and shape, on the other hand, seem to be only affected by higher salt contents. This study contributes the lacking oospore-information for both reproduction modes of European ofC. canescens– populations.
A qualitative meta-synthesis of service users’ and carers’ experiences of assessment and involuntary hospital admissions under mental health legislations: a five-year update
Authors: Bartl Gergely , Stuart Ruth , Ahmed Nafiso , Saunders Katherine , Loizou Sofia , Brady Grainne , Gray Hannah , Grundy Andrew , Jeynes Tamar , Nyikavaranda Patrick , Persaud Karen , Raad Ari , Foye Una , Simpson Alan , Johnson Sonia , Lloyd-Evans Brynmor 
Published: March 2024

AbstractBackgroundCompulsory admissions occur in psychiatric hospitals around the world. They result in coercive and sometimes traumatic experiences for service users and carers. Legal and service reforms in various countries are intended to reduce rates of detention and improve service user experience. We aimed to inform policy and service delivery by providing an up-to-date synthesis of qualitative evidence on service users’ and carers’ experiences of assessment and detention under mental health legislation, updating previous reviews in which we searched for literature published up to 2018.MethodsWe searched five bibliographic databases for studies published between January 2018 and March 2023. We identified 24 additional studies reporting qualitative investigations of service users’ or carers’ experiences of assessment or detention under mental health legislation. A team including researchers with relevant personal experience analysed and synthesised data using a thematic synthesis approach.ResultsFindings suggest that views on compulsory admissions and assessment varied: many reports highlighted its often negative, traumatic impacts on emotional well-being and self-worth, with fewer accounts of it as an opportunity to access help and support, accompanied by feelings of relief. Experiences of racial discrimination, inequality of access, and dissatisfaction with support before and after hospital stay were more prominent than in our previous reviews.ConclusionsIncreasing service user and carer involvement in treatment decisions, provision of timely information at key stages of the admission process, training of key personnel, addressing the issue of discrimination, and investing in community alternatives of inpatient care may contribute to and lead to better overall treatment experiences.
Genome-Wide Identification and Comprehensive Analysis of the SBT Gene Family in Soybean (Glycine max)
Authors: He Xuan , He Shanhong , Yang Jiaxin , Yue Zhiyong 
Published: March 2024

AbstractBackgroundThe SBT (Subtilisin-like serine protease) protein, acting as a crucial serine protease, exerts multifaceted roles in orchestrating plant growth, development, and defense responses by modulating cell wall characteristics and activities of extracellular signaling molecules. Despite extensive investigations into SBT family genes in model dicotyledonous plants like Arabidopsis, no studies have yet delved into the SBT gene family in soybean.MethodsThis study marks the inaugural comprehensive analysis of the SBT gene family in soybean, uncovering 118 GmSBT members categorized into six distinct subgroups. Additionally, we conducted comprehensive analyses covering syntenic relationships, exon-intron structures, motif compositions, and cis-regulatory elements. Our examination of RNA-seq data revealed distinct expression patterns of various GmSBTs across various above-ground tissues.ResultsIn essence, this research bridges a critical knowledge gap concerning the SBT gene family in soybean, furnishing foundational insights that will serve as a valuable reference for future investigations into soybean developmental functions and integrated data analysis.
Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasiteEntamoeba histolytica
Authors: Jiang Han , Santos Herbert J. , Nozaki Tomoyoshi 
Published: March 2024

AbstractTetraspanins (TSPANs) are a family of proteins highly conserved in all eukaryotes. Although protein-protein interactions of TSPANs have been well established in eukaryotes including parasitic protists, the role they play in parasitism and pathogenesis remains largely unknown. In this study, we characterized three representative members of TSPANs, TSPAN4, TSPAN12, and TSPAN13 from the human intestinal protozoanEntamoeba histolytica. Co-immunoprecipitation assays demonstrated that TSPAN4, TSPAN12 and TSPAN13 are reciprocally pulled down together with several other TSPAN-interacting proteins including TSPAN binding protein of 55kDa (TBP55) and interaptin. Blue native PAGE analysis showed that these TSPANs form several complexes of 120-250 kDa. Repression oftspan12andtspan13gene expression led to decreased secretion of cysteine proteases. Meanwhile, strains overexpressing HA-tagged TSPAN12 and TSPAN13 demonstrated reduced adhesion to collagen. Altogether, this study reveals that the TSPANs, especially TSPAN12 and TSPAN13, are engaged with complex protein-protein interactions and are involved in the pathogenicity-related biological functions such as protease secretion and adhesion, offering insights into the potential regulatory mechanisms of tetraspanins in protozoan parasites.
The FAM114A proteins are adaptors for the recycling of Golgi enzymes
Authors: Welch Lawrence G. , Muschalik Nadine , Munro Sean 
Published: March 2024

ABSTRACTThe Golgi apparatus contains many resident enzymes that must remain in place whilst their substrates flow through on their journey from the endoplasmic reticulum to elsewhere in the cell. COPI-coated vesicles bud from the rims of the Golgi stack to recycle Golgi residents to earlier cisternae. Different enzymes are present in different parts of the stack, and at least one COPI adaptor protein, GOLPH3, has been shown to recruit enzymes into vesicles in a specific part of the stack. We have used proximity biotinylation to identify further components of intra-Golgi transport vesicles and found FAM114A2, an uncharacterised cytosolic protein. Affinity chromatography with FAM114A2, and its paralogue FAM114A1 showed that they bind to numerous Golgi resident proteins, with membrane-proximal basic residues in the cytoplasmic tail being sufficient for the interaction. Deletion of both proteins from U2OS cells did not result in substantial defects in Golgi function. However, aDrosophilaorthologue of these proteins (CG9590/FAM114A) is also localised to the Golgi and binds directly to COPI. Generation ofDrosophilamutants lacking FAM114A revealed defects in glycosylation of glue proteins in the salivary gland. Thus, the FAM114A proteins are COPI vesicle resident proteins that bind to Golgi enzymes and so are candidate adaptors to contribute specificity to COPI vesicle recycling in the Golgi stack.
Adaptive sensor fault tolerant control with prescribed performance for unmanned autonomous helicopter based on neural networks
Authors: Wan Min , Chen Mou , Lungu Mihai 
Published: March 2024

Purpose
This paper aims to study a neural network-based fault-tolerant controller to improve the tracking control performance of an unmanned autonomous helicopter with system uncertainty, external disturbances and sensor faults, using the prescribed performance method.

Design/methodology/approach
To ensure that the tracking error satisfies the prescribed performance, the authors adopt an error transformation function method. A control scheme based on the neural network and high-order disturbance observer is designed to guarantee the boundedness of the closed-loop system. A simulation is performed to prove the validity of the control scheme.

Findings
The developed adaptive fault-tolerant control method makes the system with sensor fault realize tracking control. The error transformation function method can effectively handle the prescribed performance requirements. Sensor fault can be regarded as a type of system uncertainty. The uncertainty can be approximated accurately using neural networks. A high-order disturbance observer can effectively suppress compound disturbances.

Originality/value
The tracking performance requirements of unmanned autonomous helicopter system are considered in the design of sensor fault-tolerant control. The inequality constraint that the output tracking error must satisfy is transformed into an unconstrained problem by introducing an error transformation function. The fault state of the velocity sensor is considered as the system uncertainty, and a neural network is used to approach the total uncertainty. Neural network estimation errors and external disturbances are treated as compound disturbances, and a high-order disturbance observer is constructed to compensate for them.
Consumer purchase intention for extrinsic and intrinsic cues of construction and demolition waste recycled products
Authors: Ding Zhikun , Nie Wanqi , Tam Vivian W.Y. , Illankoon Chethana 
Published: March 2024

PurposeThe preferences and adoption of recycled materials by consumers are subject to a variety of factors, such as enablers and barriers. Despite this, there exists a paucity of research concerning stakeholders' perceived value and real purchase decision towards recycled products. Consequently, this research study aims to fill this gap by investigating stakeholders' perceived value of recycled products derived from construction and demolition (C&D) waste and its effect on purchase decisions.Design/methodology/approachResearch data were collected from 219 valid questionnaires completed by Chinese stakeholders. Structural equation modeling (SEM) was then employed to test eight hypotheses.FindingsThe results show intrinsic cue (materials) and extrinsic cue (brand) influence the stakeholders’ judgment on C&D waste recycled products’ value and then their purchase intention. However, cues such as quality, word-of-mouth, price, policy and advertised have not play a significant role in practice.Originality/valueThis research study verified the significance of brand and material cues on decision making for purchasing C&D waste recycled products, providing new insights to policy making to enhance the uptake of C&D waste recycled products in construction industry.
HR directors in India: navigating institutional incoherence for HRM stability and change
Authors: Darwish Tamer K. , Khassawneh Osama , Melhem Muntaser , Singh Satwinder 
Published: March 2024

PurposeThis paper aims to explore the strategic and evolving role of human resource management (HRM) directors within the context of underdeveloped institutional arrangements. The study focuses on India and conducts a comparative analysis of the roles of HRM directors in both multinational enterprises (MNEs) and domestic firms.Design/methodology/approachSurvey-based data from the HRM directors of 252 enterprises were gathered for the comparative analysis, including both multinational and domestic enterprises.FindingsHRM directors in MNEs lack the proficiency required to effectively fulfil their strategic role. In addition, there has been a notable shift in the responsibilities of HRM directors in MNEs, with increased emphasis on labour movements and trade union negotiations, as opposed to traditional human resource (HR) activities. This shift suggests that the role of HRM in MNEs operating in India has been influenced by local isomorphic forces, rather than following a “pendulum swing” between home and host country institutional pressures. The prevalence of informality in the Indian institutional arrangements may act as a strong counterforce to integrating the strategic agency of MNEs' home country HRM directors into the organizational structure. Despite facing resistance from the local institutional context, HRM directors in MNEs are responding with a pushback, prioritizing labour movements and trade union negotiations over core HRM activities.Research limitations/implicationsThe study highlights the broader implications for theory and practice, shedding light on the challenges faced by HRM directors in navigating incoherent institutional arrangements. It emphasizes the need for a deeper understanding of local forces in shaping HRM practices within multinational settings.Originality/valueWe contribute to the comparative HRM literature by elaborating on power struggles that HRM directors face amid the dichotomies of formal power and authority that are encoded in the organizational structure versus culturally contingent power that can be accrued from engaging in informality. We also highlight their engagement in prolonged institutional mediation and change, which serves as a compensatory mechanism for the institutional shortfalls they encounter within the context of emerging markets.
Watching performing arts encourages proenvironmental hotel choices: a moderated mediation model
Authors: Ma Jianan , Li Fangxuan (Sam) 
Published: March 2024

Purpose
Proenvironmental hotels and hotels with green initiatives are emerging as a method to address environmental issues and respond to tourists’ environmental concerns. To better understand what can encourage reservations in proenvironmental hotels, this study aims to investigate the connection between the performing arts watching experience and the preference for such a hotel.

Design/methodology/approach
Five scenario-based experiments were conducted. A total of 1,024 participants for the five studies were recruited with the help of Credamo, a commonly used Chinese data collection platform.

Findings
The results indicated that viewing performing arts could increase tourists’ preferences for proenvironmental hotels. This phenomenon occurred due to the fact that performing arts watching experience can induce a psychological state of self-transcendence in individuals, which, in turn, can raise their levels of altruism, and ultimately lead to proenvironmental hotel choices. This effect will not occur, however, when people watch performing arts with either an extrinsic motivation or in an analytical state.

Practical implications
The findings of this study provide hotel managers with a novel approach to market the proenvironmental attributes of their hotels and to promote tourists’ proenvironmental behaviors.

Originality/value
This study proposes performing arts viewing experiences as a novel way to encourage proenvironmental hotel choice. To the best of the authors’ knowledge, this is the first study to explore the impact of the performing arts watching experience on tourist behavior.