bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunological characterization of missense mutations occurring within cytotoxic T cell‐defined p53 epitopes in HLA‐A*0201+ squamous cell carcinomas of the head and neck
Authors: Ito Daisuke , Visus Carmen , Hoffmann Thomas K. , Balz Vera , Bier Henning , Appella Ettore , Whiteside Theresa L. , Ferris Robert L. , DeLeo Albert B. 
Published: February 2007

AbstractPrevious analyses of p53 in 40 HLA‐A*0201(HLA‐A2)+ squamous cell carcinomas of the head and neck (SCCHN) indicated that 6/13 p53 missense mutations that were detected, S149C, T150R, V157F, Y220C, Y220H and E271K, occurred within HLA‐A2‐restricted cytotoxic T lymphocyte (CTL)‐defined p53 epitopes. Of the 6, the p53 S149C, Y220C and Y220H peptides were immunogenic. Anti‐p53 mutant S149C and Y220H effector cells cross‐reacted against the parental wild type sequence (wt) p53 peptides, whereas anti‐p53 Y220C effector cells were specific for the mutant peptide, p53 Y220C cDNA‐transfected HLA‐A2+ SaOS cells, and an HLA‐A2+ SCCHN cell line naturally expressing the mutation. These results indicate that the p53 Y220C mutation can be processed and presented for CD8+ T cell recognition. Furthermore, using an autologous PBMC/tumor system, anti‐p53 Y220C peptide‐effector cells recognizing the autologous tumor could also be generated. Our analysis of p53 in 10 additional HLA‐A2+ SCCHN tumors detected the p53 Y220C in 2/10 tumors raising the overall frequency of the p53 Y220C mutation to 6/50 (12%) HLA‐A2+ SCCHN tumors. In contrast, independent of their HLA class I genotypes, the p53 Y220C mutation frequency for all human tumors analyzed to date is ∼1.5%. This unexpectedly high frequency of the p53 Y220C mutation in HLA‐A2+ SCCHN suggests that vaccines targeting this mutation would not only be expected to induce robust anti‐tumor immune responses in HLA‐A2+ subjects, but also be more widely applicable than previously envisioned for any given p53 missense mutation. © 2007 Wiley‐Liss, Inc.
KIT gene in pediatric osteosarcomas: Could it be a new therapeutic target?
Authors: Entz‐Werle Natacha , Gaub Marie‐Pierre , Lavaux Thomas , Marcellin Luc , Metzger Nadia , Marec‐Berard Perrine , Schmitt Claudine , Brugiere Laurence , Kalifa Chantal , Tabone Marie‐Dominique , Pacquement Hélène , Gentet Philippe , Lutz Patrick , Oudet Pierre , Babin Annie 
Published: February 2007

AbstractIn our previous study, a frequent rearrangement at 4q12 has been identified by allelotyping in our large and homogeneous population of pediatric osteosarcomas and it was significantly linked to c‐kit protein overexpression. To confirm and understand the involvement of KIT in this tumor, the next step of the study was designed to detect the potential mutations of KIT gene by sequencing the frequently mutated exons 6, 8, 10, 11, 13, 17 and 21 and, in case of unmutated samples, to confirm the genomic amplifications of the wild‐type receptor by real‐time quantitative PCR (QPCR). A new microsatellite and QPCR targeting PDGFRA was also added to check the accuracy of the 4q11‐12 locus. These techniques were performed in 74 pediatric high‐grade osteosarcomas treated with the OS94 protocol. Surprisingly, no mutations were found, but, only DNA amplification of KIT gene in the entire population. PDGFRA gene QPCR revealed an unexpected result of predominant deletions in the rearranged tumors. All these results confirm the major role of the 4q11‐12 locus and specifically the involvement of c‐kit wild‐type receptor overexpression in pediatric osteosarcomas and leads us to believe that inhibitors targeting this receptor could have a therapeutic effect in a selected group of patients. © 2007 Wiley‐Liss, Inc.
Evaluation of a new p16INK4A ELISA test and a high‐risk HPV DNA test for cervical cancer screening: Results from proof‐of‐concept study
Authors: Mao Constance , Balasubramanian Akhila , Yu Mujun , Kiviat Nancy , Ridder Ruediger , Reichert Anja , Herkert Matthias , Doeberitz Magnus von Knebel , Koutsky Laura A. 
Published: February 2007

Abstractp16INK4a, a cell cycle regulation protein, accumulates in abnormal epithelial cells infected with high‐risk human papilloma virus (HPV). In immunostaining studies, p16INK4a has shown potential as a marker of high grade cervical intraepithelial neoplasia (CIN) and invasive cervical cancer. To evaluate its potential use in cervical cancer screening, we conducted a feasibility study to compare the performance of a new enzyme linked immunosorbant assay (ELISA) for p16INK4a (mtm laboratories, Heidelberg, Germany) to that of the Hybrid capture 2™ (hc2) test for high‐risk HPV DNA for the detection of CIN3. Three hundred and nineteen women were referred from Western Washington Planned Parenthood clinics for colposcopy examination and cervical biopsy because of abnormal Pap test results. Cervical samples were obtained from study participants for p16INK4a ELISA, liquid‐based cytology and hc2. The order (first and second) for obtaining samples for cervical cytology and p16INK4a ELISA changed with every other subject. Concentrations of p16INK4a protein were higher when the sample was taken before the cytology. The sensitivity of p16INK4a ELISA (concentration ≥ 8 units/ml) taken as first sample was 90.0% for CIN3, and the sensitivity of HC2 taken as a second sample was 85%. In the same group, the specificity of p16INK4a ELISA (46.9%) was slightly better than hc2 (35.4%) Results from this proof‐of‐concept study suggest that p16INK4a ELISA has a similar sensitivity and slightly better specificity for CIN3 compared to hc2. These findings support proceeding with a larger study with samples from a population of women presenting for routine cytology screening. © 2007 Wiley‐Liss, Inc.
Avoiding calcineurin inhibitors in the early post‐operative course in high‐risk liver transplant recipients: The role of extracorporeal photopheresis
Authors: Urbani Lucio , Mazzoni Alessandro , De Simone Paolo , Catalano Gabriele , Coletti Laura , Petruccelli Stefania , Biancofiore Gianni , Bindi Lucia , Scatena Fabrizio , Filipponi Franco 
Published: February 2007

AbstractThe aim of this work is to report on the results of a single‐center, prospective study on the feasibility of calcineurin‐inhibitor (CNI)‐staggered immunosuppression by use of extracorporeal photopheresis (ECP) in liver transplant (LT) recipients at risk of renal and neurological complications.Patients were matched on a 1:1 basis with historical controls on standard CNI immunosuppression. ECP patients were treated with ECP plus antimetabolites and/or steroids, while CNIs were withheld until clinically indicated. Thirty‐six patients were evaluated: 18 ECP patients and 18 controls. ECP was tolerated in 100% of cases. CNI were introduced at a median of 8 days (4–55) in 17 ECP patients, while one patient was on a fully CNI‐sparing regimen 22 months after LT. Acute rejection occurred in 27.7% patients in ECP (5/18) versus 16.7% in controls (3/18) (P = ns) with a shorter time to rejection in ECP (36 ± 31.3 days vs. 83.6 ± 65.6 days; P = ns). All rejection episodes were amenable to medical treatment. Neurological and renal complications occurred in 22.2% (4/18) of patients in either group, but led to in‐hospital mortality in 3 patients among controls versus 1 in ECP (P = ns). One‐, 6‐, and 12‐month survival rates were 94.4, 88.1, and 88.1% in ECP versus 94.4, 77.7, and 72.2% among controls (P < 0.0001). ECP seems to allow for management of high‐risk LT recipients in the early post‐transplant course and reduction of CNI‐related mortality. Continued data validation is favored to assess the impact of ECP on long‐term graft and patient survival. J Clin Apheresis 2007. © 2007 Wiley‐Liss, Inc.
A new model for predicting the timing of leukapheresis on the basis of CD34+ cell and hematopoietic progenitor cell levels
Authors: Teng Hao‐Wei , Hsiao Liang‐Tsai , Chaou Shu‐Chou , Gau Jyh‐Pyng , Lee Tzu‐Chi , Shih Ying‐Yih , Liu Chun‐Yu , Hong Ying‐Chung , Chen Ming‐Huang , Chang Mu‐Hsin , Yang Ya‐Hsu , Chen Po‐Min 
Published: February 2007

AbstractWe developed a model (depending on peripheral CD34+ cell count and hematopoietic progenitor cell count) to determine the optimal timing of 3‐day leukapheresis in patients pretreated with chemotherapy and G‐CSF. Marrow potentials were identified on the basis of three patterns of leukapheretic yield. Pattern 1 predicted good marrow potential. The positive predictive value of a first‐day leukapheretic yield of >1 × 106 CD34+ cells/kg (mean 3‐day yield = 8.18 × 106 CD34+ cells/kg, n = 11) was 100%. Pattern 2 predicted poor marrow potential. The negative predictive value of a 3‐day leukapheretic yield of >1 × 106 CD34+ cells/kg (3‐day yield = 0.26 × 106 CD34+ cells/kg, n = 1) was 100%. Pattern 3 met neither of the above criteria (mean 3‐day yield = 1.37 × 106 CD34+ cells/kg, n = 19). The marrow potential was borderline and patients could be further divided into two subgroups according to peripheral CD34+ cell counts when WBC reached >10,000/μl. The mean yield differed significantly between pattern 1 and 3 (P < 0.001). For patients with good marrow potential, leukapheresis should begin as soon as the WBC count is >5,000/μl. Patients with borderline marrow potential may benefit from delaying leukapheresis until the WBC level is >10,000/μl and leukapheresis extended more than 3 days. J. Clin. Apheresis 2007. © 2007 Wiley‐Liss, Inc.
Differential expression and dynamic changes of SOX3 during gametogenesis and sex reversal in protogynous hermaphroditic fish
Authors: Yao Bo , Zhou Li , Wang Yang , Xia Wei , Gui Jian‐Fang 
Published: February 2007

AbstractSOX3 has been suggested to play significant roles in gametogenesis and gonad differentiation of vertebrates, but the exact cellular localization evidence is insufficient and controversial. In this study, a protogynous hermaphrodite fish Epinephelus coioides is selected to analyze EcSox3 differential expression and the expression pattern in both processes of oogenesis and spermatogenesis by utilizing the advantages that gonad development undergoes transition from ovary to intersexual gonad and then to testis, and primordial germ cells and different stage cells during oogenesis and spermatogenesis are synchronously observed in the transitional gonads. The detailed and clear immunofluoresence localization indicates that significantly differential expression and dynamic changes of Sox3 occur in the progresses of gametogenesis and sex reversal, and EcSOX3 protein exists in the differentiating primordial germ cells, oogonia, and different stage oocytes of ovaries, and also in the differentiating primordial germ cells and the Sertoli cells of testis. One important finding is that the EcSox3 expression is a significant time point for enterable gametogenesis of primordial germ cells because EcSOX3 is obviously expressed and localized in primordial germ cells. As EcSox3 continues to express, the EcSOX3‐positive primordial germ cells develop toward oogonia and then oocytes, whereas when EcSox3 expression is ceased, the EcSOX3‐positive primordial germ cells develop toward spermatogonia. Therefore, the current finding of EcSOX3 in the differentiating primordial germ cells again confirms the potential regulatory role in oogenesis and germ cell differentiation. The data further suggest that SOX3, as a transcription factor, might have more important roles in oogenesis than in spermatogenesis. J. Exp. Zool. 307A:207–219, 2007. © 2007 Wiley‐Liss, Inc.
High hydrostatic pressure treatment and storage of carrot and tomato juices: Antioxidant activity and microbial safety
Authors: Dede Saner , Alpas Hami , Bayındırlı Alev 
Published: February 2007

AbstractThe application of high hydrostatic pressure (HHP) (250 MPa, 35 °C for 15 min) and thermal treatment (80 °C for 1 min) reduced the microbial load of carrot and tomato juices to undetectable levels. Different combinations of HHP did not cause a significant change in the ascorbic acid content of either juice (P > 0.05). Both heat treatments (60 °C for 5–15 min and 80 °C for 1 min) resulted in a significant loss (P < 0.05) in the free‐radical scavenging activity as compared to untreated samples. HHP‐treated juices showed a small loss of antioxidants (below 10%) during storage. The ascorbic acid content of pressurized tomato and carrot juices remained over 70 and 45% after 30 days of storage, respectively. However, heat treatment caused a rapid decrease to 16–20%. Colour changes were minor (ΔE = 10) for pressurised juices but for heat‐pasteurised samples it was more intense and higher as a result of insufficient antioxidant activity. HHP treatment (250 MPa, 35 °C for 15 min) led to a better product with regard to anti‐radical scavenging capacity, ascorbic acid content and sensory properties (colour, pH) of the tomato and carrot juices compared to conventional pasteurisation. Therefore, HHP can be recommended not only for industrial production but also for safe storage of fresh juices, such as tomato and carrot, even at elevated storage temperatures (25 °C). Copyright © 2007 Society of Chemical Industry
Organoleptic characterization and PAH content of salmon (Salmo salar) fillets smoked according to four industrial smoking techniques
Authors: Varlet Vincent , Serot Thierry , Knockaert Camille , Cornet Josiane , Cardinal Mireille , Monteau Fabrice , Le Bizec Bruno , Prost Carole 
Published: February 2007

AbstractFour industrial processes for smoking food were studied through their effects on the organoleptic properties of smoked salmon and on the occurrence of 20 polycyclic aromatic hydrocarbons (PAHs) known as being contaminants of smoking processes. The contamination by PAHs of the food might be measured by their corresponding toxic equivalent quantity (TEQ) expressed in µg kg−1. The results show a significant correlation between the smoking process parameters, the odour of the smoked fish and the presence of PAHs. Smouldering, thermostated plates and friction smoking processes allow smoked fish with very close odorant characteristics to be obtained. However, differences of pyrolysis temperature (between 380 and 500 °C) causes significant differences of PAHs concentration even if the contents are under the legal threshold concerning benzo(a)pyrene (5 µg kg−1). Smoked fish obtained by liquid smoke vaporisation presented the lowest level of PAHs but benzo(a)pyrene concentration is nevertheless important. The odours brought by the liquid smoke process are more ‘cold smoke’ and ‘vegetal/green’ than the other techniques, which are smokier and fishier. Copyright © 2007 Society of Chemical Industry
Gene expression changes in postmortem tissue from the rostral pons of multiple system atrophy patients
Authors: Langerveld Anna Jelaso , Mihalko Daniel , DeLong Cari , Walburn Jeff , Ide Charles F. 
Published: February 2007

AbstractMultiple system atrophy (MSA) is a neurodegenerative disease characterized by various degrees of Parkinsonism, cerebellar ataxia, and autonomic dysfunction. In this report, Affymetrix DNA microarrays were used to measure changes in gene expression in the rostral pons, an area that undergoes extensive damage in MSA, but not other synucleinopathies. Significant changes in expression of 254 genes (180 downregulated and 74 upregulated) occurred in pons tissue from MSA patients when compared with control patients. The downregulated genes were primarily associated with biological functions known to be impaired in Parkinson's disease (PD) and other neurological diseases; for example, downregulation occurred in genes associated with mitochondrial function, ubiquitin‐proteasome function, protein modification, glycolysis/metabolism, and ion transport. On the other hand, upregulated genes were associated with transcription/RNA modification, inflammation, immune system function, and oligodendrocyte maintenance and function. Immunocytochemistry, in conjunction with quantitative image analysis, was carried out to characterize α‐synuclein protein expression as glial cytoplasmic inclusions in the pontocerebellar tract in rostral pons tissue and to determine the relationship between the amount of aggregated α‐synuclein protein and changes in specific gene expression. Of the regulated genes, 86 were associated with the amount of observed aggregated α‐synuclein protein in the rostral pons tissue. These data indicate that cells in the pons of MSA patients show changes in gene expression previously associated with the substantia nigra of PD patients and/or other neurological diseases, with additional changes, for example related to oligodendrocyte function unique to MSA. © 2007 Movement Disorder Society
Marcus Gunn Jaw‐Winking synkinesis in a neonate
Authors: Koelsch Emily , Harrington John W. 
Published: February 2007

AbstractThis report describes a 9‐day‐old female infant admitted for hypocalcemic seizures. The hypocalcemia was treated, but the infant was noted to have vesico‐ureteral reflux and a mild ptosis of her left eye with rhythmic blinking during bottle feeding. The eye findings were subsequently diagnosed as Marcus Gunn jaw‐winking Synkinesis. © 2007 Movement Disorder Society
Eating‐induced facial myoclonic dystonia probably due to a putaminal lesion
Authors: Gaig Carles , Muñoz Esteban , Valls‐Solé Josep , Martí María José , Tolosa Eduardo 
Published: February 2007

AbstractMyoclonic dystonia is considered a form of dystonia. We present the unusual case of a 36‐year‐old woman with HIV infection, who developed left facial myoclonic dystonia, triggered by eating in the setting of probable progressive multifocal leukoencephalopathy involving the contralateral basal ganglia. © 2007 Movement Disorder Society
Pathological laughter and crying in patients with multiple system atrophy‐cerebellar type
Authors: Parvizi Josef , Joseph Jeffrey , Press Daniel Z. , Schmahmann Jeremy D. 
Published: February 2007

AbstractIn the cerebellar type of multiple system atrophy (MSA‐C), the burden of pathological changes involves the cerebellum and its associated brainstem structures in the basis pontis and the inferior olivary nucleus, and as a result, the clinical phenotype is dominated early on by the cerebellar dysfunction. We report our clinical and post mortem findings in a patient with MSA‐C who exhibited pathological laughter in the absence of any congruent changes of mood. A review of the clinical notes of 27 other patients with MSA‐C revealed a problem with pathological laughter, or crying, or both in 9 more patients. Our finding of about 36% occurrence suggests that the problem of dysregulation of emotional expression is more prevalent in MSA‐C than the paucity of reports in the literature suggests. Our findings are consistent with the view that the cerebellum and its interconnected structures may be involved in the regulation of emotional expression. © 2007 Movement Disorder Society
New family with paroxysmal exercise‐induced dystonia and epilepsy
Authors: Kamm Christoph , Mayer Petra , Sharma Manu , Niemann Gerhard , Gasser Thomas 
Published: February 2007

AbstractTo date, there are few reports of paroxysmal exercise‐induced dystonia associated with familial epilepsy. We describe a family with 4 affected members spanning 3 generations, suggestive of autosomal‐dominant inheritance, who exhibited typical exercise‐induced dystonia, different types of epilepsy (absence and primary generalized seizures), developmental delay, and migraine in variable combinations. Linkage of the disease to loci on chromosome 2 (paroxysmal nonkinesigenic dyskinesia) and chromosome 16 (paroxysmal kinesigenic choreoathetosis, infantile convulsions with choreoathetosis) was excluded, suggesting an as yet unidentified underlying genetic basis. © 2007 Movement Disorder Society
Constantin von Economo's contribution to the understanding of movement disorders
Authors: Ransmayr Gerhard 
Published: February 2007

AbstractConstantin von Economo's (CvE) main scientific achievements were his studies on the cytoarchitectonics of the cerebral cortex, sleep, and encephalitis lethargica (EL). He found a close relationship between motor symptoms and psychiatric and behavioral disorders in EL and postencephalitic Parkinsonism and identified the underlying neuropathology in the diencephalon and the brainstem. In agreement with Tretiakoff's findings in Parkinson's disease, CvE related postencephalitic Parkinsonism to neuronal loss in the substantia nigra. Several of CvE's early, less well‐known publications also deal with the basal ganglia and movement disorders. He demonstrated in rabbits that the substantia nigra modulates automatization, coordination, and succession of masticatory movements and swallowing. In a study on the effects of experimental lesions of the cerebral peduncle in cats and monkeys, CvE hypothesized a corticotegmental pathway that maintains motor functions after pyramidal tract lesions. Recent studies have identified this pathway, which ends in the pedunculopontine nucleus. In a study on posthemiplegic chorea, CvE discussed various pathophysiological hypotheses that partly resemble modern concepts of chorea. In a clinicopathological study on Wilson's disease, CvE traced the striofugal fibers and visualized the basal ganglia outflow pathways. CvE was an outstanding multidisciplinary movement disorder specialist who contributed substantially to modern basal ganglia research. © 2007 Movement Disorder Society
Predicting the cost of Parkinson's disease
Authors: McCrone Paul , Allcock Liesl M. , Burn David J. 
Published: February 2007

AbstractThe degenerative nature of Parkinson's disease (PD) suggests that it will lead to high levels of resource use. This study measures service use and costs for a representative community sample of PD patients and identifies cost predictors. Patients were identified from general practices and were interviewed twice, separated by a 12‐month interval. Demographic and clinical data on patients were collected and 6‐month costs were calculated. Regression analysis was used to identify significant baseline predictors of follow‐up costs. The annual service costs (baseline and follow‐up combined) were £13,804 per person. Formal service costs accounted for 20% of this figure with informal care from families/friends accounting for 80%. The regression model explained 42% of total follow‐up costs and significant predictors included gender (with men having higher costs), disability, and depression. © 2007 Movement Disorder Society
Interhemispheric and ipsilateral connections in Parkinson's disease: Relation to mirror movements
Authors: Li Jie‐Yuan , Espay Alberto J. , Gunraj Carolyn A. , Pal Pramod K. , Cunic Danny I. , Lang Anthony E. , Chen Robert 
Published: February 2007

AbstractMirror movements (MM) occur in early, asymmetric Parkinson's disease (PD). To examine the pathophysiology of MM in PD, we studied 13 PD patients with MM (PD‐MM), 7 PD patients without MM (PD‐NM), and 14 normal subjects. Cross‐correlogram did not detect common synaptic input to motoneuron pools innervating homologous hand muscles in PD‐MM patients. Transcranial magnetic stimulation studies showed no significant difference in ipsilateral motor‐evoked potentials between PD‐MM patients and normal subjects. The MM side of PD‐MM patients showed a slower increase in ipsilateral silent period area with higher level of muscle contraction than the non‐MM side and normal subjects. There was less interhemispheric inhibition (IHI) at long interstimulus intervals of 20 to 50 ms in PD‐MM than PD‐NM. IHI reduced short interval intracortical inhibition in normal subjects and PD‐NM, but not in PD‐MM. IHI significantly increased intracortical facilitation in PD‐MM and PD‐NM patients, but not in normal subjects. Our results suggest that MM in PD is due to activation of the contralateral motor cortex. PD‐MM patients had reduced transcallosal inhibitory effects on cortical output neurons and on intracortical inhibitory circuits compared to PD‐NM patients and controls. These deficits in transcallosal inhibition may contribute to MM in PD patients. © 2007 Movement Disorder Society