bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variation in TCF7L2 Influences Therapeutic Response to Sulfonylureas
Authors: Pearson Ewan R. , Donnelly Louise A. , Kimber Charlotte , Whitley Adrian , Doney Alex S.F. , McCarthy Mark I. , Hattersley Andrew T. , Morris Andrew D. , Palmer Colin N.A. 
Published: July 2007

OBJECTIVE— There is considerable interindividual variation in sulfonylurea response in type 2 diabetes. Transcription factor 7-like 2 (TCF7L2) variants have been identified to be strongly associated with type 2 diabetes risk, probably due to decreased β-cell function. We hypothesized that variation in TCF7L2 would influence response to sulfonylureas but not metformin. We studied the effect of TCF7L2 rs12255372 and rs7903146 genotypes on glycemic response.
RESEARCH DESIGN AND METHODS— The DARTS/MEMO (Diabetes Audit and Research Tayside/Medicines Monitoring Unit) collaboration database includes prescribing, biochemistry, and clinical phenotype of all patients with diabetes within Tayside, Scotland, from 1992. Of these, the TCF7L2 genotype was determined in 4,469 patients with type 2 diabetes recruited to GoDARTS (Genetics of Diabetes Audit and Research Tayside) between 1997 and July 2006. A total of 901 incident sulfonylurea users and 945 metformin users were identified. A logistic regression was used with treatment failure defined as an A1C &gt;7% within 3–12 months after treatment initiation. Covariates included the TCF7L2 genotype, BMI, sex, age diagnosed, drug adherence, and drug dose. A1C pretreatment was available in a subset of patients (sulfonylurea n = 579; metformin n = 755).
RESULTS— Carriers of the risk allele were less likely to respond to sulfonylureas with an odds ratio (OR) for failure of 1.95 (95% CI 1.23–3.06; P = 0.005), comparing rs12255372 T/T vs. G/G. Including the baseline A1C strengthened this association (OR 2.16 [95% CI 1.21–3.86], P = 0.009). A similar, although slightly weaker, association was seen with rs7903146. No association was seen between metformin response and either single nucleotide polymorphism, after adjustment for baseline A1C.
CONCLUSIONS— TCF7L2 variants influence therapeutic response to sulfonylureas but not metformin. This study establishes that genetic variation can alter response to therapy in type 2 diabetes.
Characterization of oriented protein-ceramic and protein-polymer-composites for cartilage tissue engineering using synchrotron μ-CT
Authors: Zehbe Rolf , Haibel Astrid , Brochhausen Christoph , Gross Ulrich , Kirkpatrick C. James , Schubert Helmut 
Published: July 2007

Abstract
In this paper we report on the synthesis of three different gelatine based scaffold materials for the reconstruction of articular cartilage defects. The first scaffold design is based on an unmodified, oriented gelatine network, while the second design further comprises an attached inorganic hydroxyapatite layer and the third design includes poly(l-lactide) microspheres as a model material for future drug-release applications.
All three scaffold designs were characterized and imaged using synchrotron μ-CT, obtaining a complete volumetric reconstruction of a previously defined sample region.
Furthermore, two unmodified scaffolds were cultivated for one week with porcine chondrocytes. Afterwards the attached cells were labelled using a combination of Au-lysine and silver enhancer. In synchrotron μ-CT analysis we were thus able to map the cell distribution due to the difference in X-ray absorption of the labelled cells and the non labelled scaffolds in a volume of several millimetres.
Methane conversion rate into structure H hydrate crystals from ice
Authors: Susilo Robin , Ripmeester John A. , Englezos Peter 
Published: July 2007

AbstractThe methane uptake and conversion rate to structure H (sH) hydrates was measured and compared to crystallization kinetics models. Three large molecule guest substances (LMGS) were used as sH hydrate formers: neohexane (NH), methylcyclohexane (MCH), and tert‐butyl methyl ether ( TBME). The initial crystallization occurred quickly at the LMGS liquid‐ice interface until ∼20–30% of ice was converted into hydrate (hydrate growth stage I). Slower hydrate crystal growth was observed after a hydrate film covered the ice surface at a rate of 3–400 nm2/h (hydrate growth stage II). The TBME system showed the fastest kinetics at the beginning of the reaction followed by NH and MCH system. However the trend changed when the temperature was increased (“reaction” stage III). Surprisingly, the conversion rate achieved with the TBME system upon melting the ice was the smallest. This was attributed to the strong interaction of TBME with water molecules that increased the energy barrier for water molecules to form hydrate cages. The conversion rates were well correlated with the Avrami equation and the shrinking core model. Finally, NH was found to be the best LMGS in this study to obtain full conversion within a short reaction time and achieving high methane gas storage in the hydrate. © 2007 American Institute of Chemical Engineers AIChE J, 2007
Overweight and obesity in a rural amerindian population in Oaxaca, Southern Mexico, 1968–2000
Authors: Malina Robert M. , Reyes Maria Eugenia Peña , Tan Swee Kheng , Buschang Peter H. , Little Bertis B. 
Published: July 2007

AbstractThe purpose of this study was to evaluate secular change in the prevalence of overweight and obesity in a rural Zapotec Indian community in southern Mexico between 1968 and 2000. Cross‐sectional surveys of children 6–13 years, adolescents 13–17 years, and adults 19 years of age and older resident in a rural community in Oaxaca were conducted in 1968/1971, 1978, and 2000. Individuals present in the 1968, 1978, and 2000 surveys provided a small longitudinal component. Height and weight were measured; the BMI was calculated. International criteria for overweight and obesity were used. Overweight and obesity were virtually absent in school children 6–13 years in 1968 and 1978 and in adolescents in 1978. Small proportions of children (boys, 5%; girls, 8%) and adolescents (boys, 3%; girls, 15%) were overweight in 2000; two children (1%) and no adolescents were obese. Among adults, 7% of males and 19% of females were overweight and <1% of males and 4% of females were obese in 1971/1978, but 46% of males and 47% of females were overweight; and 5% of males and 14% of females were obese in 2000. The trends for children, adolescents, and adults were confirmed in the longitudinal subsamples. In conclusion, overweight and obesity are not presently a major problem in children and adolescents in this rural Zapotec community. Overweight, in particular, and to a lesser extent obesity have increased in prevalence among adults since the late 1970s. The results suggest adulthood as a critical period for onset of overweight and obesity in this sample. Am. J. Hum. Biol., 2007. © 2007 Wiley‐Liss, Inc.
Preparation of resistant starch/poly(vinyl alcohol) blend films with added plasticizer and crosslinking agents
Authors: Yoon Soon‐Do , Chough Sung‐Hyo , Park Hye‐Ryoung 
Published: July 2007

AbstractWe report on the physical properties of films synthesized with native corn starch (NCS) and resistant starch (RS4) prepared with NCS. NCS and RS4/poly(vinyl alcohol) (PVA) blend films were synthesized with a mixing process and casting method. Glycerol (GL) and citric acid (CA) were used as additives. Glutaraldehyde (GLU) was used as a crosslinking agent of the films. RS4 was synthesized with sodium trimetaphosphate and sodium tripolyphosphate as a crosslinker. Then, the RS4 thus synthesized was confirmed by the pancreatin–gravimetry method, swelling power, differential scanning calorimetry, and X‐ray diffraction. The tensile strength, elongation, swelling behavior, and solubility of the films were measured. The results of the measurements indicated that the RS4‐added film was better than the NCS‐added film. In particular, the RS4/PVA blend film with CA as an additive showed physical properties superior to those of the other films. Also, the physical properties with GLU added as a crosslinking agent to the films were investigated. With increasing GLU contents, the tensile strength increased but the elongation, swelling behavior, and solubility values of the GL‐added and CA‐added films decreased. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007
Zur Adhäsion von Wärmedämmverbundsystemen auf Holzwerkstoffplatten
Authors: Röder Jörg 
Published: July 2007

AbstractBei der Verklebung von Wärmedämmverbundsystemen auf Holzwerkstoffplatten im Holzrahmenbau kam es in der Vergangenheit zu Adhäsionsversagen. Die Schadensursachen waren bisher mangels systematischer Untersuchungen unklar. In diesem Beitrag wird der versuchstechnisch nachgewiesene Zusammenhang zwischen der Topografieänderung der Holzwerkstoffoberflächen bedingt durch den Feuchteeintrag nach dem Aufbringen des Klebemörtels und der Adhäsionsfestigkeit der Klebemörtel in Abhängigkeit von deren Rezeptur dargestellt und erläutert. Auf der Grundlage dieser Untersuchungen werden normierte Adhäsionsprognosen abgeleitet, mit denen die Haftzugfestigkeit auf den gängigen Holzwerkstoffoberflächen prognostiziert werden kann.In weiteren im Beitrag dargestellten Untersuchungen stellt sich die klimatisch bedingte jahreszeitliche Feuchtegehaltsschwankung des Holzwerkstoffs als maßgebliche Beanspruchung des Haftverbundes heraus. Abhängig von der Klebemörtelrezeptur wird daher eine zulässige Feuchteamplitude für Holzwerkstoffe abgeleitet. Die gewonnenen Erkenntnisse ermöglichen eine wissenschaftlich begründete Beurteilung des Haftverbundes zwischen Klebemörteln und Holzwerkstoffen und münden in Empfehlungen für die Ausführung von Wärmedämmverbundsystemen im Holzrahmenbau.
The Swedish rectal cancer registry
Authors: Påhlman L , Bohe M , Cedermark B , Dahlberg M , Lindmark G , Sjödahl R , Öjerskog B , Damber L , Johansson R 
Published: July 2007

Abstract

Background
An audit of all patients with rectal cancer in Sweden was launched in 1995. This is the first report from the Swedish Rectal Cancer Registry (SRCR).

Methods
Between 1995 and 2003, 13 434 patients treated for adenocarcinoma of the rectum were registered with the SRCR; there were approximately 1500 new patients annually.

Results
Approximately half had an anterior resection, a quarter an abdominoperineal resection and 15 per cent a Hartmann's procedure. The median 30-day postoperative mortality rate was 2·4 per cent and the overall postoperative morbidity rate was 35·0 per cent. The 5-year cancer-specific survival rate was 62·3 per cent. The 5-year relative survival rate was 70·1 per cent after anterior resection, 59·8 per cent after abdominoperineal resection and 39·8 per cent after a Hartmann's procedure. The crude 5-year local recurrence rate was 9·5 per cent overall, 6·1 per cent after preoperative radiotherapy and 11·4 per cent after surgery alone. For 3868 patients who had a locally curative procedure the local recurrence rate was 7·4 per cent overall, 5·9 per cent for those who had radiotherapy and 10·2 per cent for those who did not. The local recurrence rate was 2·9 per cent (28 of 968) for stage I disease, 7·9 per cent (112 of 1418) for stage II, 13·9 per cent (188 of 1357) for stage III and 8·5 per cent (45 of 532) for stage IV.

Conclusion
These good population-based results are due, in part, to the nationwide prospective quality assurance registration.
Urodynamic outcome of waterjet-assisted total mesorectal excision
Authors: Geers P , Moesta K T , Yildirim C , Thon W F , Köckerling F 
Published: July 2007

Abstract

Background
Waterjet dissection has been proposed for total mesorectal excision. The present study investigated its impact on urodynamic function and oncological outcome.

Methods
Thirty patients with rectal cancer were recruited to this prospective study, of whom 25 underwent urodynamic evaluation both before and after surgery.

Results
All patients were capable of spontaneous micturition at a median of 5 months after surgery. Urodynamic measurements revealed a decrease in detrusor pressure of more than 30 mmHg, and residual urine volumes of between 100 and 200 ml, in three patients. No patient had a complete neurogenic voiding disorder. Local recurrences developed in two of 22 patients.

Conclusion
The extent of micturition disorders observed after total mesorectal excision using the waterjet method in this small series is encouraging.
Strong Magnetic Interactions through Weak Bonding Interactions in Organometallic Radicals: Combined Experimental and Theoretical Study
Authors: Cauchy Thomas , Ruiz Eliseo , Jeannin Olivier , Nomura Mitsushiro , Fourmigué Marc 
Published: July 2007

AbstractThe magnetic properties of a series of three neutral radical organometallic complexes of general formula [CpNi(dithiolene)]. have been investigated by a combination of X‐ray crystal structure analysis and magnetic susceptibility measurements, while the assignment of the exchange coupling constants to the possible exchange pathways has been accomplished with the help of calculations based on density functional theory (DFT). The syntheses and X‐ray structures of [CpNi(adt)] (adt=acrylonitrile‐2,3‐dithiolate) and [CpNi(tfd)] (tfd=1,2‐bis(trifluoromethyl)ethene‐1,2‐dithiolate) complexes are described, while [CpNi(mnt)] (mnt=maleonitriledithiolate) was reported earlier. In the three complexes, we observed strong antiferromagnetic coupling that could not be explained solely by short S⋅⋅⋅S intermolecular contacts. Our calculations indicated that spin density in these complexes is strongly delocalized on the NiS2 moiety, with up to 20 % on the Cp ring. As a consequence, Cp⋅⋅⋅Cp and Cp⋅⋅⋅dithiolene overlap interactions have been identified as responsible for antiferromagnetic couplings. The [CpNi(adt)] complex thus has a value J=−369.5 cm−1 for an exchange interaction through a π stacking due to the Cp⋅⋅⋅Cp overlap.
Gas‐Phase Reactivity of Metavanadate [VO3]− towards Methanol and Ethanol: Experiment and Theory
Authors: Waters Tom , Wedd Anthony G. , O'Hair Richard A. J. 
Published: July 2007

AbstractThe gas‐phase reactivity of the metavanadate anion [VO3]− towards methanol and ethanol was examined by a combination of ion–molecule reaction and isotope labelling experiments in a quadrupole ion‐trap mass spectrometer. The experimental data were interpreted with the aid of density functional theory calculations. [VO3]− dehydrated methanol to eliminate water and form [VO2(η2‐OCH2)]−, which features an [η2‐C,O‐OCH2]2− ligand formed by formal removal of two protons from methanol and which is isoelectronic with peroxide. [VO3]− reacted with ethanol in an analogous manner to form [VO2(η2‐OCHCH3)]−, as well as by loss of ethene to form [VO2(OH)2]−. The calculations predicted that important intermediates in these reactions were the hydroxo alkoxo anions [VO2(OH)(OCH2R)]− (R: H, CH3). These were predicted to undergo intramolecular hydrogen‐atom transfer to form [VO(OH)2(η1‐OCHR)]− followed by η1‐O→η2‐C,O rearrangements to form [VO(OH)2(η2‐OCHR)]−. The latter reacted further to eliminate water and generate the product [VO2(η2‐OCHR)]−. This major product observed for [VO3]− is markedly different from that observed previously for [NbO3]− containing the heavier Group 5 congener niobium. In that case, the major product of the reaction was an ion of stoichiometry [Nb, O3, H2]− arising from the formal dehydrogenation of methanol to formaldehyde. The origin of this difference was examined theoretically and attributed to the intermediate alkoxo anion [NbO2(OH)(OCH3)]− preferring hydride transfer to form [HNbO2(OH)]− with loss of formaldehyde. This contrasts with the hydrogen‐atom‐transfer pathway observed for [VO2(OH)(OCH3)]−.
Prognostic significance of p16INK4a alteration for Ewing sarcoma
Authors: Honoki Kanya , Stojanovski Elizabeth , McEvoy Mark , Fujii Hiromasa , Tsujiuchi Toshifumi , Kido Akira , Takakura Yoshinori , Attia John 
Published: July 2007

AbstractBACKGROUND.Despite findings from individual studies regarding prognostic factors for Ewing sarcoma, no conclusive results have been produced, partly because of small sample sizes. The objective of the current study was to evaluate whether the presence of p16INK4a alteration is associated with a poorer prognosis in patients with Ewing sarcomas.METHODS.A review was conducted of publications that assessed associations between p16INK4a status and 2‐year survival among patients with Ewing sarcoma. The association between metastatic disease at initial diagnosis and 2‐year survival was evaluated by synthesizing data in the form of risk ratios.RESULTS.Of 11 studies that were identified in the initial search strategy, 6 studies, representing 188 patients, met the inclusion criteria and, consequently, were pooled for quantitative analyses. The estimated pooled risk ratio of p16INK4a aberration was 2.17 (95% confidence interval [95% CI], 1.55–3.03; P < .001), whereas the estimated pooled risk ratio of metastasis at diagnosis among the 164 eligible patients was 2.60 (95% CI, 1.71–3.97; P < .001). There was no statistically significant difference in the pooled estimated risk ratios of p16INK4a aberration for a poor prognosis between patients with and without metastasis at diagnosis (1.86 and 2.21, respectively; P > .59).CONCLUSIONS.The presence of p16INK4a alteration was a statistically significant predictor of prognosis for patients with Ewing sarcoma. Along with other prognostic factors, such as metastasis, the p16INK4a alteration may be a potential candidate for improving the risk‐stratifying strategy for patients with these tumors. © 2007 American Cancer Society.
Micelle–Vesicle Transition of Fatty Acid Based Ion‐Pair Surfactants: Interfacial Evidence and Influence of the Ammonium Counterion Structure
Authors: Bordes Romain , Vedrenne Marc , Coppel Yannick , Franceschi Sophie , Perez Emile , Rico‐Lattes Isabelle 
Published: July 2007

AbstractWe describe the synthesis and the physicochemical study of new ion‐pair amphiphiles from a mixture of bicyclic, cyclic, linear or branched amines and fatty acids of three chain lengths. Surface‐tension measurements of bicyclic, cyclic and branched structures of ammonium/alkanoate acid ion pairs show a phase transition, with two plateaux in the plot of surface tension versus log(c) (c=concentration). Such behaviour is related to the structure of the counterion, the alkyl chain length and the temperature. Pulsed gradient spin echo NMR spectroscopy experiments were performed to demonstrate the existence of micelles on the first plateau and to confirm the phase transition. The existence of vesicles on the second plateau of the surface tension was proved by CryoTEM observation and dynamic light scattering (DLS) measurements. Mainly, according to the structure of the counterion, there is either a strong association and a positioning along the chain leading to vesicles or a less strong association leading to external positioning and the formation of micelles at low concentrations or vesicles at higher concentrations.
Enhanced purification of cell‐permeant Cre and germline transmission after transduction into mouse embryonic stem cells
Authors: Peitz Michael , Jäger Richard , Patsch Christoph , Jäger Andrea , Egert Angela , Schorle Hubert , Edenhofer Frank 
Published: July 2007

AbstractContinuous expression of Cre recombinase has the potential to yield toxic side effects in various cell types, thereby limiting applications of the Cre/loxP system for conditional mutagenesis. In this study, we investigate the potential of Cre protein transduction to overcome this limitation. COS‐7, CV1‐5B, and mouse embryonic stem (ES) cells treated with cell‐permeant Cre (HTNCre) maintain a normal growth behavior employing Cre concentrations sufficient to induce recombination in more than 90% of the cells, whereas continuous application of high doses resulted in markedly reduced proliferation. HTNCre‐treated ES cells maintain a normal karyotype and are still able to contribute to the germline. Moreover, we present an enhanced HTNCre purification protocol that allows the preparation of a concentrated glycerol stock solution, thereby enabling a considerable simplification of the Cre protein transduction procedure. The protocol described here allows rapid and highly efficient conditional mutagenesis of cultured cells. genesis 45:508–517, 2007. © 2007 Wiley‐Liss, Inc.
η1‐Vinylidene Formation from Internal Alkynones by C–C Bond Migration
Authors: Shaw Michael J. , Bryant Steven W. , Rath Nigam 
Published: July 2007

AbstractThe [μ2‐N2{CpRu(PPh3)2}2]2+ (12+) dication is a source of the formal 16‐electron [CpRu(PPh3)2]+ fragment, and it reacts with internal alkynes such as 1,3‐diphenylpropynone, 4‐phenyl‐3‐butyn‐2‐one, and 3‐cyclopropyl‐1‐phenylpropynone to yield η1‐vinylidene complexes 2a+–2c+ by C–C bond activation. In the case of 1,3‐diphenylpropynone, the η1‐ketone complex [CpRu(PPh3)2{PhC(η1‐O)C≡CPh}]+ (3a+) was isolated as its PF6– salt, and it is apparently an intermediate in the formation of the vinylidene complex. Complexes 2a+–2c+ were characterized as their BArF4– [ArF = 3,5‐bis(trifluoromethyl)phenyl] salts by NMR and IR spectroscopic methods, ESI‐MS, and EA. The single crystal X‐ray diffraction structure of 2aBArF4 is presented. These reactions represent the first examples where an internal alkyne undergoes C–C bond migration to form an η1‐vinylidene complex. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures
Authors: Portaccio Marianna , Durante Daniela , Viggiano Andrea , Di Martino Silvana , De Luca Paola , Di Tuoro Daniela , Bencivenga Umberto , Rossi Sergio , Canciglia Paolo , De Luca Bruno , Mita Damiano Gustavo 
Published: July 2007

AbstractGlucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a function of the relative amounts of HMDA and GA. The best sensitivity was found when HMDA and GA were 5% and 2.5% in aqueous solutions, respectively. Taking as a reference the functioning of this biosensor, the roles of HMDA and GA percentages appear to be opposed when the extension of the linear response range is considered. Indeed, an increase of one unit in HMDA percentage (from 5 to 6 %) induces an increase in the extension of the linear response range equal to that obtained with a decrease of one unit of GA percentage (from 2.5 to 1.5%).
Determination of neutral carbohydrates by CZE with direct UV detection
Authors: Rovio Stella , Yli‐Kauhaluoma Jari , Sirén Heli 
Published: July 2007

AbstractA new CZE method relying on in‐capillary reaction and direct UV detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, D‐(−)‐mannitol, sucrose, D‐(+)‐fucose, D‐(+)‐cellobiose, D‐(+)‐galactose, D‐(+)‐glucose, L‐rhamnose, D‐(+)‐mannose, D‐(−)‐arabinose, D‐(+)‐xylose, and D‐(−)‐ribose. The alkaline electrolyte solution was prepared of 130 mM sodium hydroxide and 36 mM disodium hydrogen phosphate dihydrate. Separation of the sample mixture was achieved within 35 min. Calibration plots were linear in the range of 0.05–3 mM. Reproducibility of migration times was between 0.3 and 1.1%, and the detection limits for the analytes were 0.02 and 0.05 mM. The optimized method was applied for the determination of neutral monosaccharides in lemon, pineapple, and orange juices and a cognac sample. The methodology is fast since no other sample preparation except dilution is required.