bluuebunny/crossref_metadata_2025_split huggingface dataset:
LOCALIZATION OF AN ANDROGEN BINDING SUBSTANCE FROM THE RAT VENTRAL PROSTATE
Authors: Unhjem Olav , Tveter Kjell J. 
Published: December 2008

ABSTRACT
A gel filtration technique has been used for analysing the interaction of testosterone and/or its metabolites with macromolecules in the ventral prostate, rectus abdomonis muscle, liver and kidney from castrated adult male rats. Following administration of (1,2-3H)-testosterone, association of radioactivity with soluble macromolecules was demonstrated in the ventral prostate but not in the other organs analysed. A similar binding was observed when slices of ventral prostate were incubated in vitro with testosterone. When 105 000 × g supernatants from the ventral prostate were incubated with (1,2-3H)-testosterone at 37° C, radioactivity was also recovered associated with macromolecules. No association could be detected when the incubation was performed at 0° C.
CORTICOSTEROIDS AND VASCULAR CONNECTIVE TISSUE
Authors: Lorenzen Ib 
Published: December 2008

ABSTRACT
Male albino rabbits were injected with prednisone 2 mg/kg body weight, daily for two weeks. The aortic content of acid mucopolysaccharides and the synthesis of sulphated acid mucopolysaccharides decreased, whereas the collagen content remained unchanged as compared to the saline injected controls. Prednisone induced no gross or microscopic changes in the aortae. The alterations were related to those previously reported in human skin and vein biopsies and may be of pathogenic significance in the vascular fragility following long-term treatment with glucocorticoids.
INFERTILITY OF MICE INDUCED BY ANTIBODIES SPECIFIC FOR HUMAN CHORIONIC GONADOTROPHIN
Authors: Schlumberger Horst Dieter , Anderer F. Alfred 
Published: December 2008

ABSTRACT
Injections of rabbit antisera to cross-linked human chorionic gonadotrophin (HCG) into female STu mice made tolerant to rabbit γ-globulin, induced prolonged oestrus lasting for 18–60 days and phases of sterility of 37–130 days duration. When immune-competent mice were treated in a similar manner these effects were very markedly decreased due to the interference of antibodies developed against rabbit serum proteins.
The results are discussed on the basis of cross-reactions with endogenous gonadotrophins.
DER EINFLUSS VON 2-THIOURAZIL UND THIAMAZOL AUF DIE AKTIVITÄT DER L-α-GLYZEROPHOSPHAT-OXIDASE DER RATTENLEBER IN ABHÄNGIGKEIT VON L-THYROXIN, L-3′, 3,5-TRIJODTHYRONIN UND L-3′-ISOPROPYL-3,5-DIJODTHYRONIN
Authors: Seif F. J. , Guglielmi H. 
Published: December 2008

ABSTRACT
The activity of the L-α-glycerophosphate dehydrogenase (EC. 1. 1. 2. 1.) was measured in rat liver mitochondria after thyroidectomy and substitution with L-3′-isopropyl-3,5-diiodothyronine (iPT2), L-3′,3,5-triiodothyronine (T3), and L-thyroxine (T4).
A substitution with 0.18, 0.46, and 3.7 μg per 100 g animal weight and day of iPT2, T3, and T4 for 10 days was necessary to reach normal range activity. In intact rats an ED50 of 1.4. 3.4, and 15.6 μg/100 g/day of iPT2, T3, and T4 respectively was found.
2-Thiouracil and 1-methyl-2-mercapto-imidazole (methimazole, thiamazole) decreased the effectivity of T4. The effectivity of T3 was unaltered by 2-thiouracil and methimazole and that of iPT2 enhanced by 2-thiouracil and not altered by methimazole. 2-Methylmercapto-imidazole was found to be without any effect on the increased activity of L-α-glycerophosphate dehydrogenase induced by thyroxine analogues. The activation of thyroxine by deiodination and the inhibition of this reaction by 2-thiouracil and methimazole are discussed.
URINARY STEROIDS OF HEALTHY SUBJECTS FROM CHILDHOOD TO OLD AGE
Authors: Uozumi Tohru , Manabe Hisao , Tanaka Hideyuki , Hamanaka Yuji , Kotoh Kiyoshi , Suzuki Keiichiro , Matsumoto Keishi 
Published: December 2008

ABSTRACT
Urinary 17-hydroxycorticosteroids, 17-ketosteroids and 16α-hydroxydehydroepiandrosterone from eighty-six healthy subjects from the ages of 2 to 79 of both sexes were determined quantitatively by elution chromatography on cation exchange resin. Daily urinary excretions of 17-hydroxycorticosteroids and 17-ketosteroids increased from childhood to the ages of 15 to 40 and then decreased gradually with advancing ages over 60 to 79. The ratio of tetrahydrocortisone to tetrahydrocortisol decreased gradually from adolescence to old age. The ratios of 17-ketosteroids to 17-hydroxycorticosteroids and C19O2- 17-ketosteroids to total 17-ketosteroids were much higher between the ages of 20 and 40 than at other ages particularly in males. The ratios of aetiocholanolone to androsterone and tetrahydrocortisol to allo-tetrahydrocortisol seemed to be lower between the ages of 15 and 40 than at other ages. It was possible to determine the daily urinary excretions of 16α-hydroxydehydroepiandrosterone only in subjects over the age of 15, particularly between the ages of 20 and 40.
IN VITRO UPTAKE OF TRITIATED TESTOSTERONE AND OESTRADIOL BY THE LATERAL LOBE OF THE HYPERTROPHIED HUMAN PROSTATE AND THE ACCESSORY SEX GLANDS OF THE RAT – A PILOT STUDY
Authors: Jonsson Carl-Evert 
Published: December 2008

ABSTRACT
Slices of the lateral lobe of hypertrophied human prostate were incubated with tritium labelled testosterone or oestradiol.
After adding non-labelled testosterone in excess there was a marked reduction in uptake of tritiated testosterone, but neither non-radioactive oestradiol nor the antiandrogenically active Cyproterone acetate® had any effect on uptake of radioactivity. After pre-incubation with testosterone, oestradiol or Cyproterone acetate®, followed by washing and reincubation with tritiated testosterone, no blocking effect could be demonstrated.
Addition of non-radioactive oestradiol in excess did not affect the uptake of tritiated oestradiol.
In the seminal vesicles and ventral prostate of the immature rat there was an accumulation of tritiated testosterone as compared to the diaphragm. Non-radioactive testosterone in excess reduced this uptake in the target tissue.
A RADIO-BIOASSAY FOR CORTICOTROPHIN
Authors: Bukovsan William 
Published: December 2008

ABSTRACT
The increased uptake of 32P by the adrenals of the two day-old White Leghorn cockerel after corticotrophin (ACTH) stimulation was used as the end-point for the bioassay.
Early results indicated that 32P entered the blood and adrenals rapidly from a subcutaneous injection site and augmented 32P uptake of adrenals occurred after ACTH stimulation. The simultaneous injection of ACTH and 32P resulted in a rapid and sustained increase of 32P incorporation by the chick adrenal. There was a 72 % increase of 32P uptake one-half hour after ACTH stimulation; maximum incorporation, 81 %, occurred eight hours after stimulation with the hormone. The simultaneous injection of ACTH and 32P with an 8-hour corticotrophin stimulation of the adrenals and 32P uptake became the basis for the bioassay of this hormone.
The log dose-response studies showed the adrenals were sensitive to a wide range of ACTH concentrations (2.5 to 40.0 mU). The precision of this log dose-response line had a λ of 0.4762.
Pretreatment with ACTH was found to increase the sensitivity of these glands to further treatment with this hormone. Pretreatment increased the precision of this bioassay from a λ of 0.3283 to 0.1563.
THE EFFECT OF EXOGENOUS HUMAN CHORIONIC GONADOTROPHIN ON STEROID EXCRETION IN THE NEWBORN
Authors: Lauritzen C. , Shackleton C. H. L. , Mitchell F. L. 
Published: December 2008

ABSTRACT
The urinary excretion of a range of steroids and steroid-like compounds, including the major compounds with the 3β-hydroxy-Δ5 configuration, have been studied in early infancy before and after the administration of human chorionic gonadotrophin. The only compound which showed any increase in excretion was dehydroepiandrosterone which was increased in four out of seven cases studied.
THE METABOLIC SIGNIFICANCE OF THYROXINE-BINDING GLOBULIN
Authors: Bayley T. Arnold , Higgins H. Patrick , Row Vas V. , James William , Vidal Roberto 
Published: December 2008

ABSTRACT
Low thyroxine-binding globulin (TBG) capacity was found in seven members of a family in four generations. The effect on peripheral metabolism, as measured by the basal metabolic rate (BMR), of single 1000 μg intravenous doses of sodium L-thyroxine (T4) and sodium L-triiodothyronine (T3) was studied. In TBG deficient patients and those loaded with diphenylhydantoin (DPH) so that the PBI was depressed to low levels, T4 produced an effect on the BMR within 2–5 days similar in rapidity of onset and magnitude to that produced by T3 in all subjects. In normal controls or patients on maintenance DPH therapy, T4 produced little or no effect on the BMR within 8 days. The calorigenic studies were compared mainly in the TBG deficient patient with the acute (20–50 min) disappearance from plasma as well as with the chronic fate of the 131I-labelled hormones. The acute and chronic disappearances of T4 were more rapid in the TBG deficient patients whereas the acute and chronic disappearances of T3 were similar to that of subjects with normal TBG. These observations support the hypothesis that in normal humans TBG acts as a rate-regulating factor in the peripheral metabolism of acutely administered T4 by limiting the access of T4 to the tissues, thereby slowing its action on the cell. Therefore, the difference in peripheral metabolic effect of T4 and T3 would appear to be related to the presence of TBG in human plasma.
ARTIFICIAL STIMULATORY AND HORMONAL REQUIREMENTS OF DECIDUAL ALKALINE PHOSPHATASE IN THE PSEUDOPREGNANT RAT
Authors: Manning J. P. , Carter S. G. , Butler M. C. 
Published: December 2008

ABSTRACT
The influences of uterine traumatization, ovariectomy and hormone replacement therapy on decidual alkaline phosphatase were studied in pseudopregnant rats. The enzyme was also assayed in implantation sites of pregnant rats with unilateral section and ligation of the Fallopian tubes. In all pseudopregnant animals with traumatized uteri, intact or castrate receiving progesterone replacement, the enzyme activity was 12 times more active on day 8 of pseudopregnancy than the contralateral non-traumatized horn. The alkaline phosphatase reaction of day 8 pregnant rats demonstrated a similar response at the implantation site. No increased enzyme was elicited by pseudopregnant rats treated with 17β-oestradiol and/or progesterone or animals ovariectomized and traumatized on day 4 and given only oestrogen. Additionally, the changes in alkaline phosphatase from day 5 to 10 in the intact and hormone-treated, ovariectomized pseudopregnant rats with traumatized uteri were similar to those demonstrated by the intact pregnant animals, i. e., an increased reaction from day 5 to 8 and decreased activity from day 8 to 10. The data support the view that the rise in decidual alkaline phosphatase in pregnant or traumatized pseudopregnant animals is dependent on the combined effect of uterine irritation and a progesterone-conditioned endometrium.
REVERSED ACTION OF ACETYLATED CORTICOTROPHIN: INHIBITION OF ADRENO-CORTICAL SECRETION
Authors: Bulovskaya L. N. , Prokudina E. A. , Konstantinov V. L. , Tugunov S. S. , Dilman V. M. 
Published: December 2008

ABSTRACT
A derivative of corticotrophin with acetylated amino groups and hydroxyl groups in serine was used. Acetylated corticotrophin significantly decreased the 17-hydroxycorticosteroid (17-OHCS) level in the plasma of guineapigs 4 hours after the i. m. injection of the derivative in a dose equal to 2.5 USP units of the active hormone per 100 g of body weight. The mean plasma 17-OHCS concentration before the injection of the derivative was 44 ± 2.15 μg/100 ml. After the injection of acetylated corticotrophin, the fall in 17-OHCS content was highly significant, the average level being decreased to 61 %. However, in 12 out of 36 animals there was a fall to 30 % of the initial level.
The derivative obtained may be considered as an »anahormone«, and probably has the ability to inhibit the secretion of endogenous corticotrophin.
THE EFFECT OF PROTEIN DEFICIENCY ON UTERINE SENSITIVITY IN THE PSEUDOPREGNANT RAT
Authors: Kinzey Warren G. 
Published: December 2008

ABSTRACT
A purified protein-free diet was fed to Long-Evans rats during pseudopregnancy to determine the effect of protein-deficiency on the deciduoma cell response (DCR). Rats were intact, and pseudopregnancy was induced by mating with a vasectomized male. Deciduoma formation was induced by uterine traumatization on day 4 of pseudopregnancy; animals were sacrificed on day 9. In the protein-deficient group only 88 per cent of the rats reacted with positive DCR which was significantly reduced in size compared to that of control animals. Injection of oestrone and progesterone throughout pseudopregnancy resulted in formation of DCR in all protein-deficient rats. Injection of oestrone and progesterone only during the post-trauma period, but not during the pre-trauma period, raised the DCR to the level of the protein-deficient group which was injected throughout pseudopregnancy. There was no significant difference in the extent of decidual formation between uninjected protein-deficient and control animals which were sacrificed only 24 hours following uterine trauma. It is concluded that with adequate exogenous hormones uterine sensitivity to endometrial stimulus is not significantly reduced in intact protein-deficient rats. Endogenous steroids are apparently present in sufficient quantity for formation of the DCR but not for maximal growth.
INFLUENCE OF RENAL NERVES ON THE SECRETION OF ALDOSTERONE IN THE RAT
Authors: Kinson G. A. , Singer Bertha 
Published: December 2008

ABSTRACT
The effect of bilateral renal denervation on adrenocortical function has been studied in the rat. Adrenal function was assessed by measurement of the secretion rate of aldosterone and corticosterone into adrenal vein blood of anaesthetized animals by a modification of the double isotope procedure. Adrenal vein blood was collected two weeks after renal denervation or sham-denervation.
In normally fed animals renal denervation did not influence the secretion rate of aldosterone. When the animals were given a sodium deficient diet for a period of one week before collection of adrenal vein blood, the adrenal response, with respect to secretion of aldosterone, was significantly reduced, but not abolished. When the renally denervated animals were fed a sodium deficient diet the entire, two-week, post-operative period, aldosterone secretion increased to the same extent as in the shamdenervated animals. Denervation did not affect the secretion rate of corticosterone in any of these experiments.
It is concluded, on the basis of these studies on the effect of renal denervation, that renal nerves do not influence the secretion rate of aldosterone in the rat, when the diet contains a sufficient quantity of sodium. In the early stages of dietary sodium deficiency renal nerves are, in part, responsible for the increase in aldosterone secretion, while in more chronic states of sodium deficiency other, non-neural, mechanisms become more important in the regulation of aldosterone secretion.
STUDIES WITH 4-14C-LYNESTRENOL IN NORMAL AND LACTATING WOMEN
Authors: van der Molen H. J. , Hart P. G. , Wijmenga H. G. 
Published: December 2008

ABSTRACT
4-14C-Lynestrenol** was administered either orally or by intravenous injection to women using Lyndiol®*** (5 mg lynestrenol + 150 μg mestranol). In order to study the effect of the pharmaceutical form in oral administration, the radioactive progestagen was given either as the pure compound in the form of a gelatin capsule or as a tablet containing all the ingredients usually present in Lyndiol®-tablets.
The concentration of radioactivity in the blood and the excretion of radioactivity in the urine and milk of lactating women were then studied. The clearance rate of radioactivity from the blood was very low. A half-life in the order of 40 h was estimated for labelled »lynestrenol and metabolites«.
Within 4–5 days after oral administration of a 14C-lynestrenol containing tablet, 54–65 % of the radioactivity was excreted in the urine, whereas much smaller amounts (in the order of 15 % of the administered dose) were excreted following oral administration of 14C-lynestrenol taken in a capsule. After intravenous administration 45–56 % of the administered radioactivity was recovered in the urine excreted within 4 days after injection. After oral administration of a 14C-lynestrenol containing tablet to 3 lactating women 0.022. 0.028 and 0.088 per cent of the administered radioactivity was excreted in the milk collected during the first 5 days after administration.
The results indicate that resorption may be considerably influenced by the pharmaceutical form of the orally administered compound.
For oral administration of 5 mg lynestrenol daily, as a Lyndiol®-tablet, it was calculated that in the order of 1 μg (0.02 % of the administered dose) of lynestrenol or its metabolites might be excreted per 100 ml of milk. These data are discussed with regard to the amount of steroids that might be transferred to infants during breast feeding when the mothers take oral contraceptives.
OESTRIOL CONCENTRATION IN AMNIOTIC FLUID
Authors: Michie Eileen A. , Livingstone J. R. B. 
Published: December 2008

ABSTRACT
Conjugated oestriol was measured in 75 samples of amniotic fluid obtained from normal pregnancies at about 10–42 weeks gestation. There was a tenfold increase in oestriol concentration from about 20–30 weeks gestation to term. Results from 22 normal pregnancies at term gave a range of oestriol concentration of 30–182 μg/100 ml amniotic fluid. At term there was no correlation between baby weight and oestriol concentration in amniotic fluid nor between placental weight and oestriol concentration in amniotic fluid.
THE ACTION OF OESTRADIOL BENZOATE ON THE ACTIVITIES OF SOME POLYPEPTIDASES IN THE HYPOTHALAMUS OF THE PREGNANT RABBIT AFTER BLASTOCYST IMPLANTATION
Authors: Hooper K. C. , Hopkinson Patricia 
Published: December 2008

ABSTRACT
The administration of oestradiol to pregnant animals can, in certain instances, cause a delay in the onset of parturition (Hain 1935). One of the effects of excess oestradiol may be on the hypothalamus, impeding the release of oxytocin. The purpose of the present study was to note the effect of oestradiol monobenzoate on the activities of certain hypothalamic enzymes which are capable of inactivating oxytocin. In the pregnant animal, oestradiol depressed enzyme activity, and this is interpreted as indicating an inhibitory action of the hormone on the hypothalamus. The inhibitory action is opposite to that produced by the hormone in the non-pregnant animal (Hooper 1968a), and involvement of the corpus luteum or placenta seems probable.
HAND VOLUME AND SKIN THICKNESS IN A NORMAL POPULATION AND IN ACROMEGALY
Authors: Bhatia S. K. , Hadden D. R. , Montgomery D. A. D. 
Published: December 2008

ABSTRACT
The mean normal values of right hand volume and skin thickness in both sexes and in each decade (from 20–69 years) in right-handed subjects are presented. These measurements vary with age and sex. Hand volume and skin thickness showed a decrease in normal subjects above 50 years. In acromegaly there is an increase in hand volume and skin thickness, but this does not closely relate to other estimates of activity of the disease.
THE EFFECT OF TRIIODOTHYRONINE ON CERVICAL LYMPHOID STRUCTURES, THYROID ACTIVITY, IgG AND IgM IMMUNOGLOBULIN LEVEL AND EXOPHTHALMOS IN GRAVES' DISEASE
Authors: Mahaux J. E. , Chamla-Soumenkoff J. , Delcourt R. , Nagel N. , Levin S. 
Published: December 2008

ABSTRACT
The »thyroid suppression« test by l-triiodothyronine (75 μg × 14 d) used in exophthalmic goitre brings about a functional hyperstimulation of the thyroid. Thus the most sensitive criterion of thyroid activation, the serum PB131I may show a largely increased value, that is often dissociated from changes in the serum PB127I.
The clinical symptoms become worse. After a latent period, the administration of triiodothyronine appears to stimulate and to cause hypertrophy of the cervical lymphoid structures, lymph nodes and tonsils, and to induce the production of immunoglobulins: IgG including LATS and occasionally IgM.
Carbimazol treatment (3–6 months) is often able to suppress the production of LATS and restore a normal reaction to triiodothyronine.
Nevertheless, the persistence of certain active ophthalmopathies, of cervical adenopathy and, occasionally, of an elevated IgM level seems to indicate the pathological activity of a distinct autoimmune system.
SYNERGISTIC AND INHIBITORY ACTIVITY OF VARIOUS HORMONES ON THE EXOPHTHALMOS PRODUCING FACTOR IN SERA OF PATIENTS SUFFERING FROM ENDOCRINE EXOPHTHALMOS
Authors: Schemmel K. , Weisbecker L. , Müller W. , Kreysing G. 
Published: December 2008

ABSTRACT
In the carp, exophthalmos with a corresponding increase in intercorneal distance (ICD) may be induced by thyrotrophic hormone. In the range 50-500 mU TSH the effect is dose-dependent. The same effect is obtained after the injection of the serum of patients suffering from endocrine ophthalmopathia. Diluted serum does not give rise to significant exophthalmos. If, however, the carp were pretreated with β1–24-corticotrophin, diluted serum also proved to be effective. PMS-gonadotrophin and also HCG were less effective in this respect. A synergistic effect is also obtained with L-T3, whereas L-T4 shows rather inhibitory properties. A significant inhibition is obtained with diethylstilboestrol, prednisolone and D-thyroxine.
THE INFLUENCE OF TRIIODOTHYRONINE (T3) TREATMENT ON THE RESIN UPTAKE OF 131I-TRIIODOTHYRONINE
Authors: Dussault Jean , Row Vas V. , Volpé Robert 
Published: December 2008

ABSTRACT
The effects of T3 treatment on the resin uptake of 131I-T3 as well as on serum thyroxine, have been studied in a euthyroid normal control group, in a group of hypothyroid patients, and in a group of pregnant women. T3 therapy resulted in a decline in the resin uptake of 131I-T3 in the normal control group; this was considered due to a suppression of serum thyroxine (consequent to TSH suppression), with a consequent increase in the amount of unsaturated thyroxine binding globulin. In patients with hypothyroidism, however, the resin uptake of 131I-T3 actually increased as a result of T3 treatment. Since the serum thyroxine in this group could not decrease any further as a result of the T3 administration, the T3 was truly additive, saturating more TBG binding sites, thus increasing the resin uptake of 131I-T3. In the pregnant group, large doses of T3 also caused an increase in the resin uptake of 131I-T3. While the decline in serum thyroxine was only modest, it was still greater than the accompanying rise in serum T3, so that the net amount of circulating thyroid hormone was less in the post treatment phase. Thus, the rise in the resin uptake of 131I-T3 is not completely explicable in this group.