bluuebunny/crossref_metadata_2025_split huggingface dataset:
AN EFFECTIVE UPPER BOUND FOR ANTI-CANONICAL VOLUMES OF SINGULAR FANO THREEFOLDS
Authors: Jiang Chen , Zou Yu 
Published: March 2024

AbstractFor a real number
$0<\epsilon <1/3$
, we show that the anti-canonical volume of an
$\epsilon $
-klt Fano
$3$
-fold is at most
$3,200/\epsilon ^4$
, and the order
$O(1/\epsilon ^4)$
is sharp.
The Role of Personal Demands and Personal Resources in Enhancing Study Engagement and Preventing Study Burnout
Authors: Zeijen Marijntje E. L. , Brenninkmeijer Veerle , Peeters Maria C. W. , Mastenbroek Nicole J. J. M. 
Published: March 2024

Abstract
Using a 1-year longitudinal design, we examined the role of personal demands and personal resources in long-term health impairment and motivational processes among master students. Based on the job demands-resources theory and transactional model of stress, we hypothesized that students’ personal demands (i.e., irrational performance demands, awfulizing and irrational need for control) predict perceived study demands one year later, and indirectly relate to burnout. Furthermore, we predicted that personal resources indirectly associate with study engagement via students’ perceived study resources one year later. These hypotheses were tested in a sample of Dutch master students (N = 220 at T1 and T2) using structural equation modelling. As hypothesized, personal demands and personal resources at T1 predicted study demands and study resources one year later (T2, β = .25–.42, p <. 05), respectively. Study-home interference [study demand] mediated the association between personal demands and burnout (β = .08, p = .029), whereas opportunities for development [study resource] mediated the association between personal resources and study engagement (β = .08, p = .014). Hence, personal demands and personal resources relate indirectly to students’ burnout and engagement one year later via a heightened level of specific study demands and study resources. Accordingly, the present research expands the propositions of the JD-R Theory by proposing personal demands as a relevant factor for students’ long-term well-being.
Feature of Diurnal Double Maxima in the Topside Ionosphere Observed by ICON
Authors: Du Rongjin , Zhang Ruilong , Liu Libo , Han Tingwei , Li Wenbo , Tariq M. Arslan , Le Huijun , Chen Yiding , Yang Yuyan 
Published: March 2024

AbstractThis study investigates the diurnal variation of topside ionospheric plasma density using the Ionospheric Connection Explorer (ICON) observations from May to July in 2020 and 2021. The total ion density exhibits daytime double‐maxima (DDM) patterns, also known as “bite‐out” at magnetic latitudes from 10°S to 20°N and longitudes of 180°–276°E, but a single peak in other longitudes. The total ion density between 180° and 276°E reaches its first peak around 12 LT (Local Time) in both hemispheres and the second peak at 14–15 LT in the Northern Hemisphere, gradually shifting to around 16–17 LT in the Southern Hemisphere. The formation of the first peak is mainly influenced by vertical plasma drift, while the second peak is associated with both neutral wind and vertical plasma drift. Furthermore, this study provides direct observational evidence for the influence of neutral winds on the longitude and latitude differences of the topside DDM. Stronger southward magnetic meridional winds and vertical plasma drifts are observed at approximately 11–15 LT in the longitude sector with DDM compared to other longitudes, leading to a valley of ion density in the Southern Hemisphere around 15 LT. In the Northern Hemisphere, ion density continues to accumulate to form a second peak at 15 LT.
Agricultural Land‐Use Increases Carbon Yields in Lowland Streams of the Congo Basin
Authors: Drake Travis W. , Baumgartner Simon , Barthel Matti , Bauters Marijn , Alebadwa Serge , Akoko Nadine Bahizire , Haghipour Negar , Eglinton Timothy , Van Oost Kristof , Boeckx Pascal , Six Johan 
Published: March 2024

AbstractAs the dominant mode of deforestation in the Congo Basin, shifting agriculture is expected to increase with the projected four‐fold population growth for the region by 2,100. To assess how this land‐use change will affect the export of carbon (C) to rivers in a typical lowland forest ecosystem, we studied paired watersheds near Kisangani, Democratic Republic of the Congo. Two streams, one draining an intact forest (Forest) and one draining an agricultural landscape (Ag), were gauged, equipped with sensors, and sampled fortnightly for one year. Annual average specific discharge was 1.4 mm d−1 (+76%) higher in the Ag compared to the Forest. Average annual dissolved organic C (DOC) and particulate organic C (POC) concentrations were 5.2 mg L−1 (+163%) and 1.3 mg L−1 (+81%) higher in the Ag stream, which, along with the higher discharge, resulted in 8.3 (+410%) and 2.4 g C m−2 yr−1 (+97%) larger C yields, respectively. Baseflow dissolved inorganic carbon, carbon dioxide, and methane yields were also higher in the Ag stream. Despite the higher yields of organic C (OC), the composition of OC did not differ significantly. Carbon to nitrogen ratios, along with isotopic signatures, revealed that both streams contained young, semi‐degraded organic matter derived from C3 vegetation. Correspondingly, biodegradable DOC (BDOC) proportions did not differ between the streams, although the Ag stream yielded more total BDOC. These results show that agricultural land‐use likely exports a greater proportion of Net Primary Productivity (NPP) to aquatic ecosystems, which may affect both C storage in soils and the proportion of gross PP that is ultimately respired.
Axicabtagene ciloleucel treatment is more effective in primary mediastinal large B-cell lymphomas than in diffuse large B-cell lymphomas: the Italian CART-SIE study
Authors: Chiappella Annalisa , Casadei Beatrice , Chiusolo Patrizia , Di Rocco Alice , Ljevar Silva , Magni Martina , Angelillo Piera , Barbui Anna Maria , Cutini Ilaria , Dodero Anna , Bonifazi Francesca , Tisi Maria Chiara , Bramanti Stefania , Musso Maurizio , Farina Mirko , Martino Massimo , Novo Mattia , Grillo Giovanni , Patriarca Francesca , Zacchi Giulia , Krampera Mauro , Pennisi Martina , Galli Eugenio , Martelli Maurizio , Ferreri Andrés J. M. , Ferrari Silvia , Saccardi Riccardo , Bermema Anisa , Guidetti Anna , Miceli Rosalba , Zinzani Pier Luigi , Corradini Paolo 
Published: March 2024

AbstractAxicabtagene ciloleucel showed efficacy for relapsed/refractory large B-cell lymphomas (LBCL), including primary mediastinal B-cell lymphomas (PMBCL); however, only few PMBCLs were reported. Aim was to evaluate efficacy and safety of axicabtagene ciloleucel in patients with PMBCL compared to those with other LBCL, enrolled in the Italian prospective observational CART-SIE study. PMBCLs (n = 70) were younger, with higher percentage of bulky and refractory disease, compared to other LBCLs (n = 190). Median follow-up time for infused patients was 12.17 months (IQR 5.53,22.73). The overall (complete + partial) response rate (ORR,CR + PR) after bridging was 41% for PMBCL and 28% for other LBCL, p = 0.0102. Thirty days ORR was 78% (53/68) with 50% (34) CR in PMBCL, and 75% (141/187) with 53% (100) CR in other LBCL, p = 0.5457. Ninety days ORR was 69% (45/65) with 65% (42) CR in PMBCL, and 54% (87/162) with 47% (76) CR in other LBCL; progressive disease was 21% in PMBCL and 45% in other LBCL, p = 0.0336. Twelve months progression-free survival was 62% (95% CI: 51–75) in PMBCL versus 48% (95% CI: 41–57) in other LBCL, p = 0.0386. Twelve months overall survival was 86% (95% CI: 78–95) in PMBCL versus 71% (95% CI: 64–79) in other LBCL, p = 0.0034. All grade cytokine release syndrome was 88% (228/260); all grade neurotoxicity was 34% (88/260), with 6% of fatal events in PMBCL. Non-relapse mortality was 3%. In conclusion, PMBCLs achieved significantly better response and survival rates than other LBCLs.
Alpha5 nicotine acetylcholine receptor subunit promotes intrahepatic cholangiocarcinoma metastasis
Authors: Fu Yan , Shen Keyu , Wang Hao , Wang Shun , Wang Xufeng , Zhu Le , Zheng Yan , Zou Tiantian , Ci Hongfei , Dong Qiongzhu , Qin Lun-Xiu 
Published: March 2024

AbstractNeurotransmitter-initiated signaling pathway were reported to play an important role in regulating the malignant phenotype of tumor cells. Cancer cells could exhibit a “neural addiction” property and build up local nerve networks to achieve an enhanced neurotransmitter-initiated signaling through nerve growth factor-mediated axonogenesis. Targeting the dysregulated nervous systems might represent a novel strategy for cancer treatment. However, whether intrahepatic cholangiocarcinoma (ICC) could build its own nerve networks and the role of neurotransmitters in the progression ICC remains largely unknown. Immunofluorescence staining and Enzyme-linked immunosorbent assay suggested that ICC cells and the infiltrated nerves could generate a tumor microenvironment rich in acetylcholine that promotes ICC metastasis by inducing epithelial-mesenchymal transition (EMT). Acetylcholine promoted ICC metastasis through interacting with its receptor, alpha 5 nicotine acetylcholine receptor subunits (CHRNA5). Furthermore, acetylcholine/CHRNA5 axis activated GSK3β/β-catenin signaling pathway partially through the influx of Ca2+-mediated activation of Ca/calmodulin-dependent protein kinases (CAMKII). In addition, acetylcholine signaling activation also expanded nerve infiltration through increasing the expression of Brain-Derived Neurotrophic Factor (BDNF), which formed a feedforward acetylcholine-BDNF axis to promote ICC progression. KN93, a small-molecule inhibitor of CAMKII, significantly inhibited the migration and enhanced the sensitivity to gemcitabine of ICC cells. Above all, Acetylcholine/CHRNA5 axis increased the expression of β-catenin to promote the metastasis and resistance to gemcitabine of ICC via CAMKII/GSK3β signaling, and the CAMKII inhibitor KN93 may be an effective therapeutic strategy for combating ICC metastasis.
Isoform alterations in the ubiquitination machinery impacting gastrointestinal malignancies
Authors: Kasturirangan Srimathi , Nancarrow Derek J. , Shah Ayush , Lagisetty Kiran H. , Lawrence Theodore S. , Beer David G. , Ray Dipankar 
Published: March 2024

AbstractThe advancement of RNAseq and isoform-specific expression platforms has led to the understanding that isoform changes can alter molecular signaling to promote tumorigenesis. An active area in cancer research is uncovering the roles of ubiquitination on spliceosome assembly contributing to transcript diversity and expression of alternative isoforms. However, the effects of isoform changes on functionality of ubiquitination machineries (E1, E2, E3, E4, and deubiquitinating (DUB) enzymes) influencing onco- and tumor suppressor protein stabilities is currently understudied. Characterizing these changes could be instrumental in improving cancer outcomes via the identification of novel biomarkers and targetable signaling pathways. In this review, we focus on highlighting reported examples of direct, protein-coded isoform variation of ubiquitination enzymes influencing cancer development and progression in gastrointestinal (GI) malignancies. We have used a semi-automated system for identifying relevant literature and applied established systems for isoform categorization and functional classification to help structure literature findings. The results are a comprehensive snapshot of known isoform changes that are significant to GI cancers, and a framework for readers to use to address isoform variation in their own research. One of the key findings is the potential influence that isoforms of the ubiquitination machinery have on oncoprotein stability.
Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner
Authors: Wang Yujian , Luo Jingchang , Guan Xiaolu , Zhao Yan , Sun Li 
Published: March 2024

AbstractBacillus cereus is a clinically significant foodborne pathogen that causes severe gastrointestinal and non-gastrointestinal disease. Cereolysin O (CLO) is a putative virulence factor of B. cereus, and its function remains to be investigated. In this study, we examined the biological activity of CLO from a deep sea B. cereus isolate. CLO was highly toxic to mammalian cells and triggered pyroptosis through NLRP3 inflammasome-mediated caspase 1 and gasdermin D activation. CLO-induced cell death involved ROS accumulation and K+ efflux, and was blocked by serum lipids. CLO bound specifically to cholesterol, and this binding was essential to CLO cytotoxicity. The structural integrity of the three tryptophan residues in the C-terminal undecapeptide was vital for CLO to interact with membrane lipids and cause membrane perforation. Taken together, these results provided new insights into the molecular mechanism of B. cereus CLO-mediated cytotoxicity.
Regulating ion affinity and dehydration of metal-organic framework sub-nanochannels for high-precision ion separation
Authors: Mo Ri-Jian , Chen Shuang , Huang Li-Qiu , Ding Xin-Lei , Rafique Saima , Xia Xing-Hua , Li Zhong-Qiu 
Published: March 2024

AbstractMembrane consisting of ordered sub-nanochannels has been pursued in ion separation technology to achieve applications including desalination, environment management, and energy conversion. However, high-precision ion separation has not yet been achieved owing to the lack of deep understanding of ion transport mechanism in confined environments. Biological ion channels can conduct ions with ultrahigh permeability and selectivity, which is inseparable from the important role of channel size and “ion-channel” interaction. Here, inspired by the biological systems, we report the high-precision separation of monovalent and divalent cations in functionalized metal-organic framework (MOF) membranes (UiO-66-(X)2, X = NH2, SH, OH and OCH3). We find that the functional group (X) and size of the MOF sub-nanochannel synergistically regulate the ion binding affinity and dehydration process, which is the key in enlarging the transport activation energy difference between target and interference ions to improve the separation performance. The K+/Mg2+ selectivity of the UiO-66-(OCH3)2 membrane reaches as high as 1567.8. This work provides a gateway to the understanding of ion transport mechanism and development of high-precision ion separation membranes.
Meta-analysis of identified genomic regions and candidate genes underlying salinity tolerance in rice (Oryza sativa L.)
Authors: Satasiya Pratik , Patel Sanyam , Patel Ritesh , Raigar Om Prakash , Modha Kaushal , Parekh Vipul , Joshi Haimil , Patel Vipul , Chaudhary Ankit , Sharma Deepak , Prajapati Maulik 
Published: March 2024

AbstractRice output has grown globally, yet abiotic factors are still a key cause for worry. Salinity stress seems to have the more impact on crop production out of all abiotic stresses. Currently one of the most significant challenges in paddy breeding for salinity tolerance with the help of QTLs, is to determine the QTLs having the best chance of improving salinity tolerance with the least amount of background noise from the tolerant parent. Minimizing the size of the QTL confidence interval (CI) is essential in order to primarily include the genes responsible for salinity stress tolerance. By considering that, a genome-wide meta-QTL analysis on 768 QTLs from 35 rice populations published from 2001 to 2022 was conducted to identify consensus regions and the candidate genes underlying those regions responsible for the salinity tolerance, as it reduces the confidence interval (CI) to many folds from the initial QTL studies. In the present investigation, a total of 65 MQTLs were extracted with an average CI reduced from 17.35 to 1.66 cM including the smallest of 0.01 cM. Identification of the MQTLs for individual traits and then classifying the target traits into correlated morphological, physiological and biochemical aspects, resulted in more efficient interpretation of the salinity tolerance, identifying the candidate genes and to understand the salinity tolerance mechanism as a whole. The results of this study have a huge potential to improve the rice genotypes for salinity tolerance with the help of MAS and MABC.
Histopathological studies of nonhuman primates exposed to supralethal doses of total- or partial-body radiation: influence of a medical countermeasure, gamma-tocotrienol
Authors: Singh Vijay K. , Wise Stephen Y. , Fatanmi Oluseyi O. , Petrus Sarah A. , Carpenter Alana D. , Lee Sang-Ho , Hauer-Jensen Martin , Seed Thomas M. 
Published: March 2024

AbstractDespite remarkable scientific progress over the past six decades within the medical arts and in radiobiology in general, limited radiation medical countermeasures (MCMs) have been approved by the United States Food and Drug Administration for the acute radiation syndrome (ARS). Additional effort is needed to develop large animal models for improving the prediction of clinical safety and effectiveness of MCMs for acute and delayed effects of radiation in humans. Nonhuman primates (NHPs) are considered the animal models that reproduce the most appropriate representation of human disease and are considered the gold standard for drug development and regulatory approval. The clinical and histopathological effects of supralethal, total- or partial-body irradiations (12 Gy) of NHPs were assessed, along with possible protective actions of a promising radiation MCM, gamma-tocotrienol (GT3). Results show that these supralethal radiation exposures induce severe injuries that manifest both clinically as well as pathologically, as evidenced by the noted functionally crippling lesions within various major organ systems of experimental NHPs. The MCM, GT3, has limited radioprotective efficacy against such supralethal radiation doses.
Polarization-selective four-wave mixing in a degenerate multi-level system
Authors: Baek Jaeuk , Park Sanghyun , Lee Min-Hwan , Noh Heung-Ryoul , Moon Geol 
Published: March 2024

AbstractThis paper describes the first observation of polarization-selective four-wave mixing signals in conventional coupling-probe spectroscopy, specifically, saturation absorption spectroscopy in 85Rb atoms. The four-wave mixing signal is induced by two counter-propagating laser beams in a degenerate multi-level atomic system, involving the $$F_g=3 \rightarrow F_e =2,3$$

F
g

=
3
→

F
e

=
2
,
3

, and 4 transitions of the 85Rb D2 line. Consequently, the four-wave mixing signals copropagating along the probe beam induce polarization rotation of a linearly polarized probe beam. To distinguish these four-wave mixing signals from the resulting probe beam, we detect the polarization components orthogonal to the polarization direction of the input probe beam, depending on the linear polarization angles between the probe and coupling beams. The experimental findings demonstrate excellent agreement with theoretical results.
Decreased AdipoR1 signaling and its implications for obesity-induced male infertility
Authors: Kobori Toshiko , Iwabu Masato , Okada-Iwabu Miki , Ohuchi Nozomi , Kikuchi Akiko , Yamauchi Naoko , Kadowaki Takashi , Yamauchi Toshimasa , Kasuga Masato 
Published: March 2024

AbstractObesity is among the risk factors for male infertility. Although several mechanisms underlying obesity-induced male subfertility have been reported, the entire mechanism of obesity-induced male infertility still remains unclear. Here, we show that sperm count, sperm motility and sperm fertilizing ability were decreased in male mice fed a high-fat diet and that the expression of the AdipoR1 gene and protein was decreased, and the expression of pro-apoptotic genes and protein increased, in the testis from mice fed a high-fat diet. Moreover, we demonstrate that testes weight, sperm count, sperm motility and sperm fertilizing ability were significantly decreased in AdipoR1 knockout mice compared to those in wild-type mice; furthermore, the phosphorylation of AMPK was decreased, and the expression of pro-apoptotic genes and proteins, caspase-6 activity and pathologically apoptotic seminiferous tubules were increased, in the testis from AdipoR1 knockout mice. Furthermore, study findings show that orally administrated AdipoRon decreased caspase-6 activity and apoptotic seminiferous tubules in the testis, thus ameliorating sperm motility in male mice fed a high-fat diet. This was the first study to demonstrate that decreased AdipoR1/AMPK signaling led to increased caspase-6 activity/increased apoptosis in the testis thus likely accounting for male infertility.
Planctoellipticum variicoloris gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from wastewater of the aeration lagoon of a sugar processing plant in Northern Germany
Authors: Wurzbacher Carmen E. , Haufschild Tom , Hammer Jonathan , van Teeseling Muriel C. F. , Kallscheuer Nicolai , Jogler Christian 
Published: March 2024

AbstractIn the present study, we characterise a strain isolated from the wastewater aeration lagoon of a sugar processing plant in Schleswig (Northern Germany) by Heinz Schlesner. As a pioneer in planctomycetal research, he isolated numerous strains belonging to the phylum Planctomycetota from aquatic habitats around the world. Phylogenetic analyses show that strain SH412T belongs to the family Planctomycetaceae and shares with 91.6% the highest 16S rRNA gene sequence similarity with Planctopirus limnophila DSM 3776T. Its genome has a length of 7.3 Mb and a G + C content of 63.6%. Optimal growth of strain SH412T occurs at pH 7.0–7.5 and 28 °C with its pigmentation depending on sunlight exposure. Strain SH412T reproduces by polar asymmetric division (“budding”) and forms ovoid cells. The cell size determination was performed using a semi-automatic pipeline, which we first evaluated with the model species P. limnophila and then applied to strain SH412T. Furthermore, the data acquired during time-lapse analyses suggests a lifestyle switch from flagellated daughter cells to non-flagellated mother cells in the subsequent cycle. Based on our data, we suggest that strain SH412T represents a novel species within a novel genus, for which we propose the name Planctoellipticum variicoloris gen. nov., sp. nov., with strain SH412T (= CECT 30430T = STH00996T, the STH number refers to the Jena Microbial Resource Collection JMRC) as the type strain of the new species.
Gender awareness among general practitioners in France: a cross sectional study using the Nijmegen Gender Awareness in Medicine Scale (N-GAMS)
Authors: Goussault-Capmas Perrine , Panjo Henri , Pelletier-Fleury Nathalie 
Published: March 2024

AbstractGender is a key determinant of health and healthcare use. The question of whether physicians are aware of gender issues is important to avoid gender bias in medical practice. This study aimed to validate the Nijmegen Gender Awareness in Medicine Scale (N-GAMS) in a representative population of French general practitioners (GPs) and to analyze their gender sensitivity and the presence of gender stereotypes among them. The N-GAMS, already validated in medical students, measures gender awareness through 3 subscores: gender sensitivity (GS) and gender-role ideology towards patients (GRIP) and doctors (GRID) (gender stereotypes). After translation into French, it was distributed to 900 GPs. The scale was validated through exploratory factor analysis (EFA). Psychometric properties were tested. Multivariate linear regressions were conducted to explore the associations between GPs’ characteristics and N-GAMS subscores. EFA identified 3 meaningful factors consistent with prior theory. Subscores exhibited good internal consistency. The main findings were that GRIP was significantly higher in older physicians, in male physicians, among those who less involved their patients in decisions, and those who were not training supervisors. For GRID, results were quite similar to those of GRIP. GS was significantly higher for physicians working in health centres or medical homes and for those with gynecological practices but lower when they less involved patients in medical decisions. This study suggests that it is necessary to teach gender issues not only in medical schools but also as part of continuing medical education.