bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐linked glycosylation is an important parameter for optimal selection of cell lines producing biopharmaceutical human IgG
Authors: van Berkel Patrick H. C. , Gerritsen Jolanda , Perdok Gerrard , Valbjørn Jesper , Vink Tom , van de Winkel Jan G. J. , Parren Paul W. H. I. 
Published: January 2009

AbstractWe studied the variations in N‐linked glycosylation of human IgG molecules derived from 105 different stable cell lines each expressing one of the six different antibodies. Antibody expression was based on glutamine synthetase selection technology in suspension growing CHO‐K1SV cells. The glycans detected on the Fc fragment were mainly of the core‐fucosylated complex type containing zero or one galactose and little to no sialic acid. The glycosylation was highly consistent for the same cell line when grown multiple times, indicating the robustness of the production and glycan analysis procedure. However, a twofold to threefold difference was observed in the level of galactosylation and/or non‐core‐fucosylation between the 105 different cell lines, suggesting clone‐to‐clone variation. These differences may change the Fc‐mediated effector functions by such antibodies. Large variation was also observed in the oligomannose‐5 glycan content, which, when present, may lead to undesired rapid clearance of the antibody in vivo. Statistically significant differences were noticed between the various glycan parameters for the six different antibodies, indicating that the variable domains and/or light chain isotype influence Fc glycosylation. The glycosylation altered when batch production in shaker was changed to fed‐batch production in bioreactor, but was consistent again when the process was scaled from 400 to 5,000 L. Taken together, the observed clone‐to‐clone glycosylation variation but batch‐to‐batch consistency provides a rationale for selection of optimal production cell lines for large‐scale manufacturing of biopharmaceutical human IgG. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
Computer imaging: A project for pattern classification with range images
Authors: Umbaugh Scott E. , LeAnder Robert , Solt Patrick , Kharbanda Amit , Deokar Pradyna 
Published: January 2009

AbstractA series of three laboratory exercises were developed to introduce undergraduate students specializing in computer vision and image processing to three‐dimensional imaging. These lab exercises were consolidated into a term project in the Image Analysis and Computer Vision course at Southern Illinois University Edwardsville. The difficulty encountered in introducing undergraduate students to 3‐D imaging was the expense of the equipment. Because equipment expense placed a limitation on the number of 3‐D cameras available and consequently, the number of students that could use the equipment, the image database used in this project was captured by graduate students only. At the end of the term, the students evaluated the effectiveness of the project. Eighty eight percent of the students strongly agreed or agreed that they “gained insight into the process of developing algorithms for automatic object classification via images.” Additionally, 83% of the students strongly agreed or agreed that “this project is an excellent enhancement to the in‐class lectures.” © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 17: 225–231, 2009; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae20183
Audio processors as a learning tool for basic acoustics
Authors: Pejuan Arcadi , Novell Montse 
Published: January 2009

AbstractAudio processors like Audacity provide a “hear‐and‐see” learning tool for basic acoustics, combining sound and image. Activities designed as laboratory experiments with PC (even at home!) have been already successfully implemented, for example, about the dependence of timbre and acoustic spectrum, of pitch and frequency, and of loudness and amplitude. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 17: 379–388, 2009; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20207
Live problem solving via computer in the classroom to avoid “Death by PowerPoint”
Authors: Shacham Mordechai , Cutlip Michael B. , Elly Michael 
Published: January 2009

AbstractThe use of live demonstrations in courses related to numerical problem solving combined with PowerPoint presentations is advocated in order to stimulate student interest and involvement and enhance learning. The proposed approach is demonstrated by solving a system of ordinary differential equations as a boundary value problem, utilizing finite difference approximations for solving partial differential equations, and investigating ill‐conditioning in multiple linear regression. The educational benefits of this approach are discussed in detail. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 17: 285–294, 2009; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20214
Educational software to develop sheet metal with Solid Edge
Authors: del Río‐Cidoncha Gloria , Martínez‐Palacios Juan , Ortíz‐Marín Rafael , Rojas‐Sola José Ignacio 
Published: January 2009

AbstractThis article presents a useful application for university courses in Engineering Graphics, and for manufacturing processes based on developing sheet metal. From the basis of a three‐dimensional surface, the application can generate its development, as well as its area and perimeter that could be used by a conventional numerical control machine (CNC) to cut the sheet. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 17: 295–304, 2009; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20247
Biochemical Characterization of a Uranyl Ion‐Specific DNAzyme
Authors: Brown Andrea K. , Liu Juewen , He Ying , Lu Yi 
Published: January 2009

AbstractUranyl ion‐specific DNAzyme: A DNAzyme (lower strand) cleaves the substrate (upper strand) in the presence of the uranyl ion. The enzyme folds into a bulged three‐way‐junction structure with catalytically important nucleotides residing in the bulge. A highly conserved G⋅A mismatch is also crucial for the enzyme's activity.magnified imageThe biochemical characterization of a DNAzyme that is highly specific for uranyl (UO22+) ions is described. Sequence alignment, enzyme truncation, and mutation studies have resulted in a conserved sequence that folds into a bulged stem–loop structure. Interestingly, a G⋅A pair next to the scissile site is important for the uranyl ion‐specific DNAzyme; this is reminiscent of the G⋅T wobble base pair adjacent to the cleavage site that is crucial for the PbII‐specific 8–17 DNAzyme activity. Therefore wobble pairs might be important for formation of metal‐specific metal‐binding sites in DNAzymes. The DNAzyme binds the uranyl ion with a dissociation constant of 469 nM, which is among the strongest metal‐binding affinities in nucleic acid enzymes reported to date. This explains why a catalytic beacon fluorescent sensor based on this enzyme has a detection limit (45 pM) that rivals the most‐sensitive analytical instrument. It also has over 1 000 000‐fold specificity for the uranyl ion over other metal ions. The DNAzyme can carry out multiple turnover reactions that follow the Michaelis–Menten equation, with a kcat of 1.46 min−1 and a KM of 463 nM, similar to that of the 8–17 DNAzyme. The pH profile shows a bell‐shaped curve that reaches a maximum at pH 5.5, at which the in vitro selection was carried out; this suggests that in vitro selection can be performed to obtain DNAzymes with optimal performance under specific conditions under which practical applications are required. These findings enrich our fundamental understanding of metal‐binding sites in nucleic acids and allow the design of sensors with better performance.
Phase Transformation in Titania Nanocrystals by the Oriented Attachment Mechanism: The Role of the pH Value
Authors: Ribeiro Caue , Barrado Cristiano M. , de Camargo Emerson R. , Longo Elson , Leite Edson R. 
Published: January 2009

AbstractThe oriented attachment (OA) mechanism has been investigated as an important process in the formation of anisotropic nanostructures such as depicted. The results showed that the control of a desired phase in this system may be attained by the control of OA mechanism through pH value, obtaining several morphologies.magnified imageA new synthetic method for TiO2 nanocrystals starting from metallic Ti and hydrogen peroxide was developed, in order to obtain minimal interferences to evaluate phase transformation in the system. The results revealed that the crystal morphology appeared to be dictated by the pH value, which shows a strong dependence on the surface energy. The involvement of the oriented attachment (OA) mechanism is important to modify the morphology and, hence, the distribution of the surface energy and confirmed that the mechanism can accelerate certain phase transitions, albeit pH dependence in terms of how the mechanism affects the final particle morphology and direction of crystalline growth. The importance of the mechanism was also apparent in extremely basic conditions, which indicates a possible correlation with the formation of hydrogen titanate nanostructures.
Intramolecular Methylacridan–Methylacridinium Complexes with a Phenanthrene‐4,5‐diyl or Related Skeleton: Geometry–Property Relationships in Isolable CH Bridged Carbocations
Authors: Suzuki Takanori , Yoshimoto Yasuyo , Takeda Takashi , Kawai Hidetoshi , Fujiwara Kenshu 
Published: January 2009

AbstractBridging the gap: Snapshots of 1,6‐H‐shift precursors indicate that a narrower CH⋅⋅⋅C+ separation (D in the ORTEP diagram) in the title complexes induces faster degenerate rearrangement of 1+. A contact distance of less than 2.7 Å is necessary to realize the organic three‐center two‐electron bond of [CHC]+, as indicated by extrapolation of the X‐ray data.magnified imageThe isolation and low‐temperature X‐ray analyses of a series of intramolecular methylacridan–methylacridinium complexes have been achieved. The two chromophores are in close proximity due to an arylene spacer, such as a phenanthrene‐4,5‐diyl or biphenyl‐2,2′‐diyl unit. These bridged carbocations prefer the CH localized structure both in solution and in the solid state. The bridging hydrogen atom undergoes a facile intramolecular hydride shift from one carbon atom to another in solution, and the energy barrier is linearly correlated with the intramolecular C⋅⋅⋅C+ distance in the solid‐state geometry, as determined by single‐crystal X‐ray analyses. By extrapolation from the data, the delocalized three‐center bond of [CHC]+ would be formed when the C⋅⋅⋅C+ distance is less than 2.7 Å.
Highly Water‐Soluble, Fluorescent, Conjugated Fluorene‐Based Glycopolymers with Poly(ethylene glycol)‐Tethered Spacers for Sensitive Detection of Escherichia coli
Authors: Xue Cuihua , Velayudham Singaravelu , Johnson Steve , Saha Ratul , Smith Adrian , Brewer Wilbel , Murthy Pushpalatha , Bagley Susan T. , Liu Haiying 
Published: January 2009

AbstractKnow your bacteria! Two fluorene‐based, conjugated polymers with oligo(ethylene glycol)‐ and poly(ethylene glycol)‐tethered spacers have been prepared by the Suzuki coupling polymerization reactions. β‐Glucose and α‐mannose residues were covalently attached to the conjugated polymers by post‐polymerization functionalization with thiol‐functionalized carbohydrates under basic conditions. Investigations on their use as biosensing materials for the detection of Escherichia coli are reported (see figure).magnified imageTwo bromide‐bearing, fluorene‐based, conjugated polymers with oligo(ethylene glycol)‐ and poly(ethylene glycol)‐tethered spacers have been prepared by the Suzuki coupling polymerization of bromide‐bearing, fluorene monomers. β‐Glucose and α‐mannose residues have been covalently attached to the conjugated polymers by post‐polymerization functionalization of the precursor polymers with thiol‐functionalized carbohydrates under basic conditions through thioether linkage. A glucose‐bearing glycopolymer with oligo(ethylene glycol)‐tethered spacers (polymer A) displays poor water solubility. However, glycopolymers with poly(ethylene glycol)‐tethered spacers (polymers B and C) are highly water‐soluble due to their long, flexible, hydrophilic spacers. Incubation of the ORN178 strain of Escherichia coli (E. coli) with α‐mannose‐bearing glycopolymer (polymer C) results in the formation of fluorescent cell clusters, causing significant red shifts in UV/Vis absorption and fluorescent spectra of the polymer through multivalent cooperative interactions of the polymeric carbohydrates with the bacterial pili. In contrast, polymer C displays no interactions with a mutant ORN208 strain of E. coli.
A General Route to Construct Diverse Multifunctional Fe3O4/Metal Hybrid Nanostructures
Authors: Guo Shaojun , Dong Shaojun , Wang Erkang 
Published: January 2009

AbstractMultifunctional nanostructures: By using 3‐aminopropyltrimethoxysilane as a linker, Au nanoparticles (NPs), Au shells, flowerlike Au/Pt hybrid NPs, and Ag or Au/Ag core/shell NPs could be supported on the surface of superparamagnetic Fe3O4 spheres to construct hybrid nanostructures that display near‐IR absorption, high catalytic activity towards an electron‐transfer reaction, or excellent surface‐enhanced Raman scattering activity. The picture shows SEM images of Fe3O4 spheres coated with Au shells (top) and with Au/Pt hybrid NPs (bottom).magnified imageWe have developed a simple, efficient, economical, and general approach to construct diverse multifunctional Fe3O4/metal hybrid nanostructures displaying magnetization using 3‐aminopropyltrimethoxysilane (APTMS) as a linker. High‐density Au nanoparticles (NPs) could be supported on the surface of superparamagnetic Fe3O4 spheres and used as seeds to construct Au shell‐coated magnetic spheres displaying near‐infrared (NIR) absorption, which may make them promising in biosensor and biomedicine applications. High‐density flowerlike Au/Pt hybrid NPs could be supported on the surface of Fe3O4 spheres to construct multifunctional hybrid spheres with high catalytic activity towards the electron‐transfer reaction between potassium ferricyanide and sodium thiosulfate. High‐density Ag or Au/Ag core/shell NPs could also be supported on the surface of Fe3O4 spheres and exhibited pronounced surface‐enhanced Raman scattering (SERS), which may possibly be used as an optical probe with magnetic function for application in high‐sensitivity bioassays.
Asymmetrically Substituted Benzene‐1,3,5‐tricarboxamides: Self‐Assembly and Odd–Even Effects in the Solid State and in Dilute Solution
Authors: Stals Patrick J. M. , Smulders Maarten M. J. , Martín‐Rapún Rafael , Palmans Anja R. A. , Meijer E. W. 
Published: January 2009

AbstractMolecular organization: Chiral benzene tricarboxamides with methyl substituents at defined positions self‐assemble into supramolecular polymers of preferred helicity by three‐fold α‐helical‐type hydrogen bonding. The odd–even effect is operative and all derivatives are liquid crystalline showing a Colho phase (see figure).magnified imageAsymmetric benzene‐1,3,5‐tricarboxamides (aBTAs) comprising two n‐octyl and one chiral methyl–alkyl side chain were synthesised and characterised. The influence of the position and the configuration of the chiral methyl group (methyl at the α, β or γ position) in the aliphatic side chains on the liquid‐crystalline properties and the aggregation behaviour of the aBTAs was systematically studied and compared to symmetrical benzene‐1,3,5‐tricarboxamides (sBTAs). Solid‐state characterisation (polarised optical microscopy, IR spectroscopy, X‐ray diffraction and differential scanning calorimetry) revealed that all aBTAs show threefold, α‐helical‐type intermolecular hydrogen bonding between neighbouring molecules and exhibit a columnar hexagonal organisation from room temperature to well above 200 °C. Moving the chiral methyl group closer to the amide group stabilises the liquid‐crystalline state, as evidenced by a higher clearing temperature and corresponding enthalpy. The self‐assembly of dilute solutions of the aBTAs in methylcyclohexane (≈10−5 mol L−1) was investigated with circular dichroism (CD) spectroscopy. The sign of the Cotton effect demonstrated a pronounced odd–even effect, whereas the value of the molar ellipticity, Δε, in the aBTAs was independent of the position of the methyl group. Subsequent temperature‐dependent CD measurements showed that the aggregation of all aBTAs can quantitatively be described by the nucleation‐growth model and that the stability of the aggregates increases when the chiral methyl group is closer to the amide moiety. The results presented herein illustrate that even small changes in the molecular structure of substituted benzene‐1,3,5‐tri‐carboxamides affect their solid‐state properties and their self‐assembly behaviour in dilute solutions.
ChemInform Abstract: Synthesis of Novel Amino Acid Derived Sulfinamides and Their Evaluation as Ligands for the Enantioselective Transfer Hydrogenation of Ketones.
Authors: Zani Lorenzo , Eriksson Lars , Adolfsson Hans 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: TBOxCrIIICl‐Catalyzed Enantioselective Synthesis of 1,3‐Butadien‐2‐ylcarbinols.
Authors: Naodovic Marina , Xia Guoyao , Yamamoto Hisashi 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 5‐Substituted 3‐Nitro‐1‐trinitromethyl‐1,2,4‐triazoles.
Authors: Kofman T. P. , Kartseva G. Yu. , Glazkova E. Yu. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Properties of 5‐Substituted 1‐Dinitromethyl‐3‐nitro‐1H‐1,2,4‐triazoles.
Authors: Kofman T. P. , Trubitsin A. E. , Dmitrienko I. V. , Glazkova E. Yu. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Copper Catalyzed 1,3‐Dipolar Cycloaddition Reaction of Azides with N‐(2‐Trifluoroacetylaryl)propargylamines. A Mild Entry to Novel 1,4‐Disubstituted‐[1,2,3]‐triazole Derivatives.
Authors: Medebielle Maurice , et al. et al. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of α‐[5‐(5‐Amino‐1,3,4‐thiadiazol‐2‐yl)‐2‐imidazolylthio]acetic Acids.
Authors: Hadizadeh Farzin , Vosooghi Rahil 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Multicomponent Reactions in Chiral Ionic Liquids: A Stereocontrolled Route to Mercaptopyranothiazoles.
Authors: Yadav Lal Dhar S. , Yadav Beerendra S. , Rai Vijai K. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 1,3‐Dioxanes Catalyzed by TsOH—SiO2 under Solvent‐Free Conditions.
Authors: Borah Kalyan Jyoti , Phukan Mridula , Borah Ruli 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A One‐Pot Biginelli Synthesis of 3,4‐Dihydropyrimidin‐2‐(1H)‐ones/thiones Catalyzed by Triphenylphosphine as Lewis Base.
Authors: Debache Abdelmadjid , Amimour Mouna , Belfaitah Ali , Rhouati Salah , Carboni Bertrand 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Design of Multi‐Binding‐Site Inhibitors, Ligand Efficiency, and Consensus Screening of Avian Influenza H5N1 Wild‐Type Neuraminidase and of the Oseltamivir‐Resistant H274Y Variant.
Authors: Garcia‐Sosa Alfonso T. , Sild Sulev , Maran Uko 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Morphology of and Catalysis in Single Crystals of Zeolites
Authors: Schoonheydt Robert A. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Palladium and Gold—Palladium Catalysts for the Direct Synthesis of Hydrogen Peroxide
Authors: Edwards Jennifer K. , Hutchings Graham J. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Neutron Diffraction Study of Multiferroics LnMn2O5
Authors: Radaelli P. G. , Chapon L. C. 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Synthesis of Indoles from Alkynes.
Authors: Krueger Karolin , Tillack Annegret , Beller Matthias 
Published: January 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.