bluuebunny/crossref_metadata_2025_split huggingface dataset:
Omission of axillary dissection without radiation in breast cancer patients with one macrometastatic sentinel lymph node undergoing mastectomy
Authors: Ogiya Akiko , Sakai Takehiko , Nakadaira Uta , Miyagi Yumi , Takahata Chikako , Abe Tomomi , Inoue Yuka , Yoshida Kazuyo , Uehiro Natsue , Maeda Tetsuyo , Takahashi Yoko , Inari Hitoshi , Kataoka Akemi , Takano Toshimi , Osako Tomo , Ueno Takayuki 
Published: February 2025

Abstract

Background
This study is an ad hoc analysis of a single-center trial that investigated the oncologic safety of omission of axillary lymph node dissection (ALND) in breast cancer patients who had one macrometastasis in sentinel lymph nodes (SNs). Herein, we report the outcomes of patients who underwent mastectomy without ALND, had one macrometastasis in SNs, and did not receive radiotherapy (RT).
Materials and Methods
The study group comprised 136 patients with cTis-3N0 who underwent mastectomy and had one macrometastasis in SNs and who did not receive ALND nor RT. For comparison, a historical control group of 67 patients who received mastectomy and ALND without RT and had one nodal macrometastasis in SNs was used. Both groups received systemic therapy. The primary endpoint was distant disease-free survival (DDFS), and secondary endpoints were ipsilateral axillary lymph node recurrence rate, and overall survival (OS). Outcomes were compared using the Kaplan–Meier method and log-rank test.
Results
The median duration of follow-up in the study group was 73.5 months. The five-year DDFS was comparable in the two groups (study group: 95.5%; control group: 95.4%; p = 0.74). The five-year ipsilateral axillary lymph node recurrence rate was 0.8% and 0%, respectively (p = 0.80). There was no significant between-group difference regarding the five-year OS rate (99.2% and 96.9%, respectively, p = 0.91).
Conclusion
Omission of ALND without RT could be a promising approach in patients who have one macrometastasis in SNs and undergo mastectomy with systemic therapy.
Trial registration
The trail has been registered in UMINCTR on March 19, 2014 (Registration No. UMIN000013059).
A High-Efficiency Single Layer Slot Array Antenna with GGW Pillbox Feeding System
Authors: Mohammadpour Mohammad , Mohajeri Farzad , Parizi Seyed Ali Razavi 
Published: February 2025

Abstract

This paper presents a slot array antenna composed of H-plane horns as radiating slots and a pillbox feeding mechanism in gap waveguide (GW) technology. This new configuration presents a new full-metal slot array antenna with low complexity, fabrication cost, and high gain and directive radiation. The proposed fully metallic antenna leverages a design that offers high efficiency and a flat gain response over the desired bandwidth. A fabricated prototype exhibits 70–90% efficiency, an impedance bandwidth of about 8% (S11 < -10 dB), and a 2.5 dB gain variation across the operating bandwidth.
Novel Aryl Substituted sulfonyl piperazine derivatives: Tandem one-pot multi component synthesis via palladium catalyzed Suzuki-Miyaura cross coupling, structure studies and evaluation of anticancer activity
Authors: S jeevan Chakravarthy A , A Shriraksha , R Sreenatha N , N Sharadamma , R Nagesh Babu 
Published: February 2025

Abstract

Novel N,N-substituted biphenyl sulfonyl piparazines were synthesized through a tandem one-pot reaction of N-substituted piparazines with p-bromosulfonyl chloride in DCM, followed by the palladium catalyzed cross coupling with aryl boronic acids at room temperature and atmospheric pressure. The N-p-bromosulfonylpiparazine intermediate was isolated and crystals obtained through slow evaporation technique was subjected to single crystal diffraction studies to get the structural insights. The study revealed the crystallization in triclinic lattice with P1 space group with each asymmetric unit comprising of two molecules A & B in a nonplanar geometry. The piperazine ring in each molecule was seen to have a puckering environment with Chair conformation. Hirshfeld surfaces mapped on dnorm property and two-dimensional fingerprint graphs provided the types and extent of contribution of intermolecular interaction present in the crystal. The relevant two-dimensional fingerprint graphs revealed H…H, H…C, H…O, and H…Br to be the main forces leading to the crystallization of the intermediate. The novel biphenyl N-sulfonyl piperazine compounds obtained by the Suzuki-Miyaura cross coupling reaction were isolated, characterized and subjected to insilico and invitro anticancer investigations. Biological activity prediction and in-silico ADMET analysis performed using Swiss ADME and ProTox-II tools gave an insight into the pharmacokinetic properties and safety profiles of the synthesized molecules. Synthesized compounds were predicted to have high gastrointestinal absorption and termed safe for organ toxicity parameters. Also, molecular docking studies were conducted using Autodock Vina and Discovery Studio to predict and analyse the binding affinity and interaction of the novel piperazine molecules with survivin protein and human B-DNA. Compound 5a showed highest binding affinity of -8.2 kcal/mol with survivin. Docking of compounds 5 with DNA showed binding affinity of -9.6 kcal/mol. In addition, the cytotoxic effect of the molecules on U87 (glioblastoma) and OVCAR3 (ovarian carcinoma) cell lines, by MTT assay demonstrated appreciable results, suggesting them as prospective candidates for anticancer agents.
Patch-CLIP - Contrastive Health Record-Image Joint Training with Patch Embedding Loss
Authors: Bhat Sheethal , Mansoor Awais , Georgescu Bogdan , Zinnen Mathias , Sahu Pranjal , Panambur Adarsh B. , Ghesu Florin C. , Grbic Sasa , Maier Andreas 
Published: February 2025

Abstract

Vision-Language (VL) models such as Contrastive Language-Image pretraining (CLIP) use multimodal self-supervised learning (SSL) methods to extract maximal information from large-scale datasets. This enables the trained model to learn key image encodings while correlating them to corresponding textual information through a contrastive loss function that maximizes the similarity of VL pairs. Due to weak supervision provided by the text information, these VL models demonstrate strong zero-shot classification performance; however, their performance on downstream tasks such as object detection and localization remains suboptimal [1]. In this work, we introduce a novel contrastive loss function that aligns image patch embeddings with text embeddings. These patch embeddings that are usually discarded output from the image encoder can naturally incorporate location information during unsupervised training. The proposed approach improves both localization and classification performance, thus allowing key findings to be localized without the need for a complex downstream object detection framework. We evaluated the performance of our proposed method on two chest X-ray (CXR) datasets for abnormality detection and localization tasks. The experiments achieved state-of-the-art (SOTA) results for 8 abnormality detection tasks. Moreover, the patch prediction maps introduced in work considerably reduce False Positive (FP) rates at a given sensitivity, compared to saliency maps.
Assessment of Irrigation Water Quality of the River Cauvery and Its Distributaries in Tamil Nadu, India through Hydrochemical Analysis
Authors: Sharma Rakesh 
Published: February 2025

Abstract

The river Cauvery and its tributaries are vital for irrigation in Tamil Nadu, India, but face pollution from anthropogenic activities, raising concerns about water suitability. This study analyzed water samples from the river Cauvery and its distributaries in the Tiruchirappalli district across four seasons to assess irrigation suitability using hydrochemical parameters. The results showed that parameters such as Electrical Conductivity (EC), Sodium Adsorption Ratio (SAR), and Residual Sodium Carbonate (RSC) were within permissible limits for irrigation. The Koolayar channel exhibited slightly elevated chemical levels compared to other distributaries in both summer (EC: 960 µS/cm, Cl: 174 mg/l, Ca: 120 mg/l & Na: 112 mg/l) and winter (EC: 996 µS/cm, Cl: 149 mg/l & Na: 106.6 mg/l). Piper diagram analysis revealed that chloride dominance, particularly in summer season. Overall, the river Cauvery and its distributaries remain suitable for irrigation despite localized pollution. These findings support sustainable water management and agricultural practices in the study region.
Assessing the cross transferability of finger millet SSR markers and genetic diversity analysis in browntop millet (Brachiaria ramosa L. Stapf) based on microsatellite data and morphological traits
Authors: Rahul G.K , Bhavani  , C Nandini , L Ravi Kumar R. 
Published: February 2025

Abstract

Browntop millet (Brachiaria ramosaL. Stapf) holds cultural and agricultural significance in Southern India, serving as both a staple food for humans and fodder for livestock. Recently, its high fiber and protein content has gained public attention, making it popular as a nutritious grain. However, despite its nutritional benefits, the limited genomic information available for browntop millet has hindered breeding efforts. To address this knowledge gap, an assessment was made to test the transferability of SSR (Simple Sequence Repeat) primers from finger millet to browntop millet, aiming to identify genetic polymorphism. In this study, 27 accessions of browntop millet were subjected to SSR analysis using 100 SSR primers developed for finger millet. Among the 100 primers tested, five were mono-nucleotide, 38 were di-nucleotide, 42 were tri-nucleotide, and 15 were tetra-nucleotide repeats. Initial screening of 100 primers on two browntop millet accessions GPUBT-2 and IC617953 showed 40 per cent and 42 per cent transferability. Among the 30 amplified primers screened on both browntop millet genotypes, one was a mono-nucleotide repeat, 16 were di-nucleotide repeats, and 13 were tri-nucleotide repeats. The analysis revealed that di-nucleotide repeats were the most common repeat type. 30 primers which showed amplification in both genotypes were used for screening 27 browntop millet genotypes. The PIC values of all the polymorphic loci for 25 browntop millet genotypes varied from 0 to 0.68, major allele frequency varied from 0.40 to 1, allele number varied from 1 to 5 and genetic diversity varied from 0 to 0.72. Cluster analysis combining morphological and molecular markers based on UPGMA method resulted in two major clusters. These findings demonstrate the potential for utilizing genomic resources from related millet species and highlight opportunities to enhance browntop millet breeding programs.
Exploring Host-pathogen and Trichoderma strains Interaction in Lentil genotypes for the Management of Wilt
Authors: Namdev Ayushi , Tiwari Neha , Kumar Tapan , Johare Juhi , Saxena D R , Saxena Moly 
Published: February 2025

Lentil wilt, attributed to Fusarium oxysporum f. sp. lentis, presents an enduring challenge in lentil farming. As agricultural practices veer towards sustainable disease management, investigating the intricate relationship between host plants, pathogen and antagonists, has become a focal point. In this regard, the present study suggests bio-control potential of three Trichoderma strains in four cultivated and nine new genotypes of lentil. T. viride exhibited maximum 67.57 per cent inhibition of F. oxysporum f. sp. lentis, (FOL) followed by T. harzianum strain-2 (65.48%) in dual culture assay. In field conditions, T. harzianum strain 1 displayed the maximum effectiveness, showing the minimum average wilt incidence 16.37 %, followed by T. harzianum strain-2 (19.28%) as compared to the control (35.18%). Furthermore, seed treatment with T. harzianum strain-2 significantly promoted root length, recorded average plant highest 10.67 cm in the cultivated genotypes, as compared to the control (9.09 cm). Similarly, the plant height, was also enhanced reaching up to 19.59 cm due to this treatment, followed closely by T. harzianum strain-1 (19.32 cm), while in control it was significantly less i.e. 16.95 cm. Overall plant weight was also highest 22.47 gm in T. harzianum strain-1 treated lentil seeds, followed by T. harzianum strain-2 (21.61 gm), superior to control (13.29 gm). Studies on endophytic nature of both the species of Trichoderma indicated that their colonization in genotypes is host dependent, the maximum 73 % was in LIRL 22-109 followed by ILL 10847 (69 %), this association is positively correlated with wilt incidence and significantly lowered down the wilt incidence 7.85 % and 9.45 %, respectively. Compatibility assessment between Trichoderma strains and fungicides exhibited high compatibility of T. harzianum strain-2 (45.00 mm) and T. harzianum strain-1 (44.00 mm) with Propineb 70% WP. These interaction between Trichoderma strains and the lentil wilt signifies a hopeful integrated strategy for the disease suppression
Quality Evaluation of Various Millets and Other Grains Used in Traditional Indian Cuisine and Study of Their Nutritional Potential
Authors: Jamane Shamli R. , Kathale Payal S. , Sharma Shital K. , Agnihotri Adarsh K. 
Published: February 2025

The evolution of food took place over time, along with the development of agricultural practices. Various species of food grains were identified, which significantly strengthen the food chain. Millets were used as the main ingredients of a daily diet, but over time, their use gradually decreased. Initially, the side effects of modern foods were not felt, but with an increase in lifestyle diseases, humans started realizing the bad effects of modern foods, which forced re-identification, protection, and revival of healthy foods. As a result, the health benefits of millets and other grains were re-identified and re-established. With the above facts, an attempt has been made to compare the nutritional strength of wheat with that of some important millet and other flours, focusing on their potential health benefits. This comparative study dealt with the evaluation of millet and other flours like rajgira or amaranth ramdana (Amaranth - Amaranthus caudatus L.), ragi ;finger millet - Eleusine coracana (L.) Gaertn.], jowar (sorghum - Sorghum bicolor L. Syn. Sorghum vulgare), bajra (pearl millet - Pennisetum glaucum (L.) R. Br.), singhada (chestnut - Trapa natans L.), chawal (rice - Oryza sativa L.), makka (maize – Zea mays L.) and gehu (wheat - Triticum aestivum L.) flour. An attempt has been made for standardization of various flours, primarily as per BIS and FSSAI guidelines, including moisture, pH, total and acid insoluble ash, bulk density, water and oil absorption capacity, swelling and foaming index, foam stability, emulsion stability, and emulsion activity, and a comparative assessment of their nutritional facts like total fat, total carbohydrates, dietary and crude fiber, calories, caffeine, protein, gluten, cholesterol, and sodium energy content, to reach conclusive recommendations. The obtained result revealed millets and rice are equal or high in possessing nutritional values compared to wheat flour and provide a significant contribution to a routine diet as a staple food.
A note on Radula onraedtii Yamada, a poorly known liverwort species from Western Ghats, India
Authors: Verma Praveen , Alam Afroz 
Published: February 2025

In present paper a poorly known species of genus Radula, R. onraedtii Yamada of Marchantiophyta from Nilgiri hills, Western Ghats is described and illustrated in bryo-flora of Western Ghats. This species is very little and scattered in distribution and is known only from very few localities of Arunachal Pradesh in Eastern Himalaya and Western Ghats in Indian bryoflora.
Inhibition of Shiga Toxin 2 for E. coli O157 Control: An In-Silico Study on Natural and Synthetic Compounds
Authors: Ali Ashiq , Noor Isra , Shaukat Maleeha , Waheed Warda , Akbar Kaynaat , Ji Ziyi , Su Zhongjing 
Published: February 2025

Introduction/Objectives:
Escherichia coli strains are known to cause various
gastrointestinal disorders, with Shiga toxin 2, a potent cytotoxin, being a key virulence
factor contributing to disease severity. Targeting Shiga toxin 2 presents a promising approach
for therapeutic intervention in controlling E. coli O157 infections. This study
aims to explore natural and synthetic inhibitors as potential therapeutic agents against
Shiga toxin 2 through in-silico molecular docking and drug-likeness predictions.

Methods:
An in-silico molecular docking study was conducted using AutoDock Vina
and Chimera to assess the binding affinity of various natural and synthetic inhibitors
against Shiga toxin 2. The selected inhibitors were evaluated for their drug-likeness
based on adsorption, distribution, metabolism, and excretion (ADME) properties, applying
Lipinski's rule of five and the Boiled-Egg technique to predict their suitability as potential
drugs in biological systems.

Results:
During the screening process, luteolin, a natural flavonoid, exhibited the highest
binding affinity to Shiga toxin 2, with a notable negative binding energy of -8.7 kcal/-
mol, indicating strong interaction potential.

Conclusion:
The findings suggest that luteolin holds promise as a lead molecule for further
development as a therapeutic agent against E. coli infections, warranting additional
studies to validate its efficacy and safety.
Crippled Hepatocarcinogenesis Inhibition of Quercetin in Glycolysis Pathway with Hepatic Farnesoid X Receptor Deficiency
Authors: zhong Wusheng , Chen Tao , Chen Ling , Xing Yaqi , Lin Haorui , Xie Shuli , Nawaz Mateen , Huang Danmei , Huang Zhanqin , Lu Jun , Chen Zhiming , Niu Yongdong 
Published: February 2025

Aim:
Quercetin, a bioactive flavonoid extracted from traditional Chinese medicine, has antihepatocellular
carcinoma effects. Farnesoid X receptor (FXR), a nuclear receptor highly expressed in the liver,
plays important roles in maintaining hepatic glucose homeostasis, anti-inflammation, liver regeneration,
and anti-cancer properties. Whether quercetin regulates the glycolysis/glycolysis pathway through FXR signaling
remains unknown.

Methods:
KEGG Enrichment, GO Enrichment, Protein-Protein Interaction (PPI) Network, Molecular Docking,
and RNA-Seq Analysis (Swiss Target Prediction, GeneCard databases, Kaplan-Meier Plotter, etc). Cell
activity, cell proliferation, and cell cycles were separately analyzed by CCK-8 assay, clone formation assay,
and flow cytometry. QRT-PCR determined the mRNA levels of related genes in response to quercetin. HPLCMS/
MSHPLC-MS/MS determined the metabolite profiles. FXR deficiency Hep3B cells were used for discriminating
the quercetin’s effects with or without FXR.

Results:
Quercetin-related genes were significantly correlated with FXR in hepatocarcinogenesis, especially
in glycolysis. The top 30 related genes between FXR, quercetin, and glycolysis were enriched and chosen to
further study. Furthermore, the strongest binding energy determined by the molecular docking model of between
quercetin and FXR was -6.55 kcal/mol. Quercetin inhibited cell proliferation by the accumulation of
Hep3B cells in the S-phase. The differential expressed genes (C-MYC, PCNA, CYCLIN-D1, and P21) associated
with glycolysis were observed. Furthermore, quercetin also inhibited the expression of HK2, GAPDH,
and LDHA. Meanwhile, the levels of glycolysis/gluconeogenesis-related metabolites were regulated by quercetin.

Conclusion:
Quercetin makes an essential anti-HCC effect by crippling the glycolysis/gluconeogenesis process
via FXR signaling.
Efficacy of Roxadustat in Anemia with Chronic Kidney Disease
Authors: Lin Yongda , Liao Chunlin , Zheng Lingqian , Chen Wenmin , Zhou Tianbiao , Li Tianyu 
Published: February 2025

Introduction:
Anemia is one of the complications of chronic kidney disease (CKD), and its incidence
gradually increases as the disease progresses. A major cause of such anemia is the severe erythropoietin
deficiency in CKD. Therefore, improvement in red blood cell production is a choice of treatment. Erythropoiesis-
stimulating agents (ESAs) have been used for the therapy of anemia induced in CKD. Roxadustat is a novel
drug for improving such anemia. This study aimed to compare the efficacy of roxadustat and ESAs in patients
with CKD.

Material and Methods:
A retrospective analysis of CKD patients with anemia who regularly use roxadustat
and ESAs in the Nephrology Department of the Second Affiliated Hospital of Shantou University School of
Medicine was carried out. Baseline clinical data and clinical indicators during the medication period were
collected and analyzed in both groups.

Results:
In this retrospective study, we found that roxadustat was effective in improving anemia. The anemia
rate, Hb<11g/dL, before treatment in patients with CKD was 94.55%. The standard rate of patients with CKD
anemia was 25.45% after one month of treatment, 54.54% after three months of treatment, and 65.45% after
six months of treatment. In the clinical observations of the medication, roxadustat did not differ from ESAs in
the treatment of anemia in CKD. However, roxadustat did not improve micro-inflammatory status or increase
serum potassium.

Conclusion:
Roxadustat did not differ from ESAs in the treatment of anemia in CKD patients. However, it
has the potential to lower blood lipids and improve iron deficiency.

Clinical Trial Registration Number:
ChiCTR2300071087.
Study on the Mechanism of Anti-Atopic Dermatitis by Herba Siegesbeckiae Based on Metabolomics
Authors: Wang YingYue , Chen Xiaowei , Zhang Lingling , Chen Yuting , Xu Yubin , You Chunxue , Lu Xuetao 
Published: February 2025

Background:
Atopic dermatitis is a common inflammatory skin disease worldwide
that is characterized by skin barrier dysfunction, itching, and a reduced quality of life.

Objective:
The research at hand aimed to delve into the anti-atopic dermatitis mechanism of
Herba Siegesbeckiae, a traditional medicinal herb, using a metabolomic approach.

Methods:
The molecular mechanism by which Herba Siegesbeckiae acts against atopic dermatitis
was investigated by establishing a mouse model of atopic dermatitis while conducting a
metabolomics analysis on its metabolites.

Results:
Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine
levels in serum, participating in inhibiting itching and regulating immunity signaling were found
to be restored to varying degrees in AD treating with HS. A total of 31 differential metabolites
were selected from metabolomics results, among which N-acetyl-L-alanine (VIP = 1.62), Nacetyl-
L-methionine (VIP = 1.5), uracil (VIP = 1.47), and prostaglandin E2 (VIP = 1.4) play important
roles in the anti-AD regulatory mechanisms of HS and can be used as biomarkers. In addition,
the mechanisms of HS anti-AD have been shown to be associated with seven metabolic
pathways, including β-alanine metabolism, glycerophospholipid metabolism, histidine metabolism,
and so on.

Conclusion:
In conclusion, HS demonstrated properties that counteract Atopic Dermatitis by
suppressing itchiness and boosting the immune system, subsequently controlling the concentrations
of related metabolites.