bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of cortisone with and without heparin on angiogenesis induced by prostaglandin e1 and by s180 cells, and on growth of murine transplantable tumours
Authors: Ziche Marina , Ruggiero Marco , Pasquali Franca , Chiarugi Vincenzo P. 
Published: February 2007

AbstractCortisone acetate, locally applied in sustained‐release pellets, is effective in inhibiting angiogenesis induced by prostaglandin EI in the rabbit cornea. The inhibitory effect of cortisone is not increased by addition of heparin. Similar results were obtained with angiogenesis induced by S180 cells. The effects of cortisone with and without heparin were also studied on 5 transplantable murine tumours: 3 variants of B16 melanoma, Lewis lung carcinoma and fibrosarcoma M4. The effect of cortisone in slowing the growth rate of tumours was modestly potentiated by heparin, but no regression of the tumour mass occurred.
Selective cytotoxicity of murine monoclonal antibody LAM2 against human small‐cell carcinoma in the presence of human complement: Possible use for in vitro elimination of tumor cells from bone marrow
Authors: Stahel Rolf A. , Mabry Mack , Sabbath Kert , Speak Jonathan A. , Bernal Samuel D. 
Published: February 2007

AbstractLAM2 is a murine IgM monoclonal antibody (MAb) which binds strongly to the cell membrane of human lung small‐cell carcinoma (SCC) and squamous‐cell carcinoma but not to normal bone‐marrow cells. The cytotoxicity of this antibody in the presence of human complement was investigated in vitro by chromium release and clonogenic assays. The optimal treatment conditions included incubation with antibody for 30 min at 37°C followed by 3 additions of human complement 30 min apart. Cell lysis ranged from 94 to 98% in 4 SCC cell lines at antibody dilutions of 1: 100: a lower level of lysis (60%) occurred in a lung squamous cell carcinoma cell line. The cytotoxic effect was strictly complement‐dependent. No cytotoxic effect was seen with other human cell lines including lung adenocarcinoma, lung large‐cell carcinoma, myeloid leukemia, and lymphoblastic leukemia. No lysis was seen with nucleated marrow cells from healthy volunteers. Normal marrow cells in excess did not inhibit SCC cell lysis. Incubation with antibody and complement resulted in a 100‐fold reduction of colony formation of SCC cells, but did not reduce the number of colonies of marrow‐cell precursors, including CFU‐GEMM, BFU‐E, and CFU‐C. The selective cytotoxicity of LAM2 antibody to SCC, but not to normal bonemarrow cells, suggests that this antibody may be useful for in vitro elimination of SCC cells from the bone marrow.
Oesophageal cancer studies in the caspian littoral of Iran: Some residual results, including opium use as a risk factor
Authors: Ghadirian P. , Stein G. F. , Gorodetzky C. , Roberfroid M. B. , Mahon G. A. T. , Bartsch H. , Day N. E. 
Published: February 2007

AbstractA study was conducted in northern Iran in areas of very high, high and moderately low incidence of oesophageal cancer. Morphine metabolites in urine as an indicator of opium use, and a variety of nutritional and biochemical measures, including salivary antipyrine half‐life, were determined in households with a case of oesophageal cancer and in control households. Results on 1,590 individuals showed that the prevalence of appreciable levels (≤ 1μ/ml) of urinary morphine metabolites was much higher in areas of high and very high incidence of oesophageal cancer than in low‐incidence areas, particularly for those under age 50 years in both sexes, where a 6‐fold difference was seen. Members of households with a case of oesophageal cancer had a higher prevalence of positive urinary morphine metabolite findings than members of control households from the same village. Salivary antipyrine half‐life (AP‐T 1/2), as a measure of hepatic drug metabolizing capacity, was estimated in 120 subjects. No relationship was seen with use of opium, region of residence, case‐control status of household, age or sex. Experimental studies have meanwhile identified mutagenic substances with carcinogenic potential in opium pipe scrapings, used widely in the study region, opium pyrolysates and morphine pyrolysates. Our data give additional support to the hypothesis that opium use, in the form of its pyrolysates, is one of the factors involved in the aetiology of oesophageal cancer in the region.
Use of novel chemical supplements in the establishment of three human malignant lymphoma cell lines (NU‐DHL‐1, NUDUL‐1, and NU‐AMB‐1) with chromosome 14 translocations
Authors: Epstein Alan L. , Variakojis Daina , Berger Carol , Hecht Barbara K. 
Published: February 2007

AbstractThree new cell lines have been established from patients with malignant lymphoma utilizing a human diploid feeder layer, pooled human serum, and the chemical supplements L‐cysteine, iron‐saturated transferrin, and bathocuproine disulfonate, a copper chelator. After a short period of growth, the 3 cell lines were successfully weaned from the feeder layers but continued to require human serum and the chemical supplements for up to 9 months of culture. The cell lines are currently grown in RPMI–1640 medium and fetal calf serum without further supplementation. The NU‐DHL‐I cell line was established from the involved lymph node of a 73‐year‐old White male with diffuse large‐cell lymphoma. The cell line expresses cytoplasmic IgM/lambda heavy and light chains, is Epstein‐Barr virus (EBV)‐negative, and is positive for several B‐cell markers, indicating that it is derived from a mature‐B‐cell neoplasm. The NU‐DUL‐1 cell line was established from the cerebrospinal fluid of a 42‐year old White male with undifferentiated lymphoma, non‐Burkitt's type, who initially presented with a mediastinal mass and had subsequent involvement of the central nervous system. The cell line is EBV‐negative, but surprisingly it is positive for early B‐cell markers. The NU‐AmB‐1 cell line was established from the abdominal mass of a 12‐year‐old Hispanic male with undifferentiated lymphoma, Burkitt's type. The cell line is EBV‐positive and expresses early B‐cell markers. All 3 cell lines are aneuploid or pseudodiploid and contain chromosome 14q+ abnormalities including a newly described complex translocation t(?; 1; 8; 14) in the NU‐AmB‐1 cell line. The establishment of these cell lines was made possible by refinements in the cell culture of the human malignant lymphoma. The availability of well‐characterized lymphoma cell lines with specific chromosomal translocations will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Gangliosides in human chronic lymphocytic leukaemia
Authors: Anh‐Tuan Nguyen , Pick Judit , Vajda János , Harsányi Veronika , Hollán Susan R. 
Published: February 2007

AbstractGangliosides were analysed by elaborated overpressured thin‐layer chromatography (OPTLC) in 6 cell samples from 5 patients with chronic lymphocytic leukaemia (CLL) of B‐cell origin, and 4 individual lymphocyte preparations from normal blood donors. The principal difference in the ganglioside profile between these two counterparts appears to be the presence of GD3 and the predominance of the less polar compounds (GM3, GM2, GM1) in CLL cells. Qualitatively, GD3, accounted for about 5. 5% of the total CLL gangliosides, whereas it was not detectable in normal lymphocytes. Quantitatively, GM3 constituted more than 81% of the total CLL gangliosides, a proportion more than twice as high as that found in normal lymphocytes. Three other minor gangliosides were isolated from CLL cells; these were shown to be GM2 (trace amounts), GM1 (7. 7%), and GD1 (4%). The expression of individual gangliosides varied greatly among the various CLL samples obtained from different patients, and even from the same patient, if examined at different times. No gangliosides were found in the supernatant collected from CLL cells subjected to a temperature shift (0°C to 37°C) or in the cell‐free medium harvested from the CLL cells kept in overnight culture.
N‐ras gene activation in the rd human rhabdomyosarcoma cell line
Authors: Chardin Pierre , Yeramian Patrick , Madaule Pascal , Tavitian Armand 
Published: February 2007

AbstractA transforming N‐ras allele was molecularly cloned from the RD human rhabdomyosarcoma cell line, and the nature of its activation studied. Construction of chimeric recombinants between the RD‐transforming allele and a normal human allele enabled us to localize the alteration responsible for the activation to the second exon of the N‐ras gene. The nucleotide sequence of this exon, when compared to that of the normal allele, revealed a single difference at the 61st amino acid position of the encoded protein; the CAA codon for glutamine in the normal allele was mutated to a CAT codon for histidine in the RD‐transforming allele. This result is the first description of a histidine replacing glutamine in the 61st position and provides further evidence that the 61st amino acid is one of the preferential sites for N‐ras activation.
Cell DNA content — Correlation with clonogenicity in the human tumour cloning system (HTCS)
Authors: Verheuen R. H. M. , Feitz W. F. J. , Beck J. L. M. , Debruyne F. M. J. , Vooys G. P. , Kenemans P. , Herman C. J. 
Published: February 2007

AbstractThirty‐six ovarian and renal‐cell tumours were analyzed by flow cytometry (FCM) for DNA content and in parallel were assayed for colony formation in a human tumour cloning system (HTCS). While 15/19 (79%) tumours with an abnormal (aneuploid) DNA stemline formed colonies in the HTCS, only 2/17 (12%) of diploid tumours formed colonies. All samples contained tumour cells as assessed by routine cytological examination. The capacity to form colonies in the HTCS was not correlated in these tumours with grade or stage of disease or tumour type. The level of aneuploidy expressed as the FCM DNA index did not correlate with the cloning efficiency in HTCS. These findings suggest that tumour growth in the HTCS reflects a biologically important potential, related in at least some turnours to an abnormal DNA stemline.
Phenotypic interconversion of B16 melanoma clonal cell populations: Relationship between metastasis and tumor growth rate
Authors: Stackpole Christopher W. , Fornabaio Donna M. , Alterman Andrea L. 
Published: February 2007

AbstractThree distinct dissemination‐related phenotypes have been recognized in clones of the mouse B 16 melanoma based on in vivo behavior: metastatic (spontaneously disseminating to the lungs from solid tumors), colonizing (capable of forming tumor colonies in the lungs following intravenous injection), and null (tumorigenic but non‐metastatic and non‐colonizing). From a progenitor null clone, G3, subclones that became phenotypically diversified in vitro (metastatic G3.5 and null G3.15) and in vivo (metastatic G3.12 and colonizing G3.26) were derived. During long‐term culturing, G3 cells became metastatic and then lost that activity, G3.5 and G3.12 cells gradually lost metastatic activity, and G3.26 cells became slightly metastatic and non‐colonizing. Subclone G3. I5 became highly metastatic after a single subcutaneous (s. c.) tumor passage. In aged mice, and in young mice injected with incompletely‐tumorigenic cell doses, G3 and G3.26 s. c. tumors were metastatic, but cells cultured from those tumors or metastases were non‐metastatic when tested in young mice at standard highly‐tumorigenic cell doses. The behavior of G3.5 and G3.12 tumors was not altered in aged mice or when tumors were initiated with small cell inocula. Analysis of growth characteristics associated with these phenotypic interconversions indicated that lung‐colonizing potential was directly related to the ability of the cells to grow as multicell colonies in 0.3% agar, and that metastatic activity was expressed by tumors that grew at moderate rates. In young mice receiving standard cell doses, G3.5 and G3.12 tumors inherently grew at that rate, whereas G3 and G3.26 tumors grew more rapidly and G3.15 tumors grew more slowly. Regardless of inherent phenotype, all clones were capable of expressing metastatic activity, at least transiently, as tumor growth was altered to moderate rates. Expression of metastatic behavior might, therefore, be regulated to some extent by tumor growth characteristics.
Clonal analysis of cytotoxic T‐lymphocyte response to autologous human metastatic melanoma
Authors: Anichini Andrea , Fossati Giuseppe , Parmiani Giorgio 
Published: February 2007

AbstractPeripheral blood lymphocytes (PBL) from a melanoma (Me) patient, previously shown to be unable to react against the autologous tumor (Me 28) after mixed lymphocyte‐tumor culture (MLTC), were cultured in vitro with the autologous tumor in MLTC and/or with IL‐2‐containing supernatants. T‐cell clones were then obtained by limiting dilution and by micromanipulation. Eleven clones, selected for autologous tumor (Auto‐Tu) cytotoxicity, were tested for specificity on a panel of 17 cell cultures of normal and neoplastic origin, revealing a complex spectrum of lytic activities. Three groups of clones could be identified depending on the patterns of cytotoxicity. One clone (B11.12) lysed Me28 and expressed a borderline reactivity against one allogeneic Me. A second group of clones (A4, A4.18, H10, E12, C9) lysed the Auto‐Tu and allogeneic Me. The last group of clones (A4.2, A4.3, A4.4, A7, B7) expressed a broader pattern of reactivity with significant cytotoxicity against targets of different histologic origin. Furthermore, the second and third groups of clones lysed K562 while B11.12 did not. The Auto‐Tu‐restricted reactivity of clone B11.12, confirmed by a further test on 13 allogeneic Me and on autologous IL‐2 cultured lymphocytes, suggests the recognition of antigenic structures preferentially expressed on Me28. Blocking studies, performed with monoclonal antibodies (MAb), revealed that an anti‐HLA class 1 MAb (w6/32), but not two anti‐DR MAbs (L243, D1.12), could inhibit the cytotoxic activity of clones B11.12 on Me28. A significant blocking effect by w6/32 on Me28 was achieved also with clones A4.4 and H10 but not with clones A4.2, A4.3 and A7. The phenotype of all clones was T3+, T4−, T8+, HNK‐1−, suggesting that the anti‐tumor effectors were of the T‐cell lineage. Taken together, these data indicate that it is possible to isolate anti‐tumor CTL‐clones after MLTC from a PBL population of a metastatic melanoma patient. Furthermore, we present evidence suggesting a role of class‐1 antigens in the interaction of some cloned effectors with the autologous tumor target.
Familial and environmental interactions in bladder cancer risk
Authors: Kantor Arlene F. , Hartge Patricia , Hoover Robert N. , Fraumeni Joseph F. 
Published: February 2007

AbstractIn a population‐based study of 2,982 bladder cancer patients and 5,782 controls in 10 geographic areas of the United States which was designed to assess the role of environmental risk factors, information was also obtained on the history of urinary tract cancer in first‐degree relatives. A family history of urinary tract cancer significantly elevated the risk of bladder cancer [relative risk (RR) = 1.45], with higher risks observed among patients under age 45. The risks of bladder cancer associated with positive family history were generally higher among persons with suspected environmental exposures, particularly heavy cigarette smoking (RR = 10.7 among those who smoked 3 or more packs per day). Further studies of bladder cancer should incorporate biochemical and genetic probes to assess mechanisms of familial susceptibility and interactions with environmental factors.
Combined recombinant human interferon alpha2 and cytotoxic agents studied in a clonogenic assay
Authors: Welander Charles E. , Morgan Timothy M. , Homesley Howard D. , Trotta Paul P. , Spiegel Robert J. 
Published: February 2007

AbstractA human tumor clonogenic assay has been used to test the antiproliferative effect of recombinant human leukocyte interferon alpha2 alone and in combination with each of 8 cytotoxic agents. Cell lines derived from 6 human tumors and primary tumor cells from 13 patients have been used in these clonogenic assay studies. Results show that interferon as a single agent causes insignificant reduction in tumor cell colony survival if the short‐term 1‐hr cell exposure method is used; only high concentrations of interferon used in continuous cell exposure in the clonogenic assay can demonstrate a reduction in colony survival to below 50% of control values. Combinations of interferon with either doxorubicin or cisplatin frequently show additive and occasionally synergistic antiproliferative effects on tumor cell colony formation. Variations in drug concentrations and sequencing of drugs have been tested, showing that optimal antiproliferative effects of combined interferon and doxorubicin are realized when maximal concentrations of interferon and prolonged cell exposure time of both interferon and doxorubicin are employed. Combinations of interferon and doxorubicin tested in the clonogenic assay demonstrate cytotoxicity superior to that of either agent tested alone.
Oncogene expression in human tumors
Authors: Tatosyan A. G. , Galetzki S. A. , Kisseljova N. P. , Asanova A. A. , Zborovskaya I. B. , Spitkovsky D. D. , Revasova E. S. , Martin P. , Kisseljov F. L. 
Published: February 2007

AbstractThe expression of 9 oncogenes in primary tumors and in human tumors passaged in nude mice was tested: a total of 28 tumor types was analyzed. Oncogenes src, fps, and mos were not expressed in any of the tumors tested but oncogene myc was transcribed in most of the tumors and myc was overexpressed in 3 tumors passaged in nude mice (Ewing sarcoma, large intestine carcinoma and kidney carcinoma) and in primary fibrous histiocytoma. Enhanced transcription of ras and fos genes was observed nonspecifically in different tumors. Oncogene sis was activated specifically in metastases of different tumors in lymph nodes.
Decreased calcium dependence of lymphoblastoid cell lines compared with burkitt lymphoma cell lines
Authors: Parsons P. G. , Moss D. J. , Morris C. , Musk P. , Maynard K. , Partridge R. 
Published: February 2007

AbstractThe extracellular calcium level required for proliferation was compared in B lymphoid cell lines from various sources by determining the calcium concentration at which long‐term proliferation was inhibited by 50% (CaPD50). Fourteen Burkitt lymphoma (BL) lines had a mean CaPD50 of 44 ± 28 μM whereas 45 lymphoblastoid cell lines (LCLs) obtained by in vitro transformation of B lymphocytes with Epstein‐Barr virus (EBV) had a mean CaPD50 of 3.6 ± 1.8 μM. This difference applied also to autologous BL lines and LCLs established from the same patient. The decreased calcium requirement of virally‐transformed compared with tumour‐derived cell lines therefore appears to be a universal phenomenon in mamalian cells. Within the BL group, no correlation was found between the calcium requirement for proliferation and presence or absence of the EBV genome. Arrest of BL lines and LCLs occurred in the G1 phase of the cell cycle and was readily reversed by additional of calcium to the medium. One anomalous LCL was found which showed a high CaPD50 (43 ± 6 μM) and accumulated in both G1 and G2. These results, in combination with a previous study of EBV transformation in vitro, indicate that the calcium dependence of B lymphocytes generally decreases in the following order: normal cells > BL cells = early stage transformation > LCL. The 2 transformed phenotypes thus distinguished in human lymphoid cells may offer unique opportunities for defining the status and expression of EBV in vitro and in vivo.
Mononuclear cells infiltrating human mammary carcinomas: Immunohistochemical analysis with monoclonal antibodies
Authors: Hurlimann Jean , Saraga Puica 
Published: February 2007

AbstractBreast carcinomas were examined by the immunoperoxidase technique using antisera specific for lymphocyte subsets, monocytes, NK cells and major histocompatibility antigens (HLA‐A,‐B,‐C; la‐like). Sixty‐four per cent of the patients had a moderate or strong mononuclear cell infiltration, 77% of the patients without mononuclear cell infiltration had receptors for estrogens as compared to 51% of the patients with infiltration. The majority of the infiltrating mononuclear cells were T cells; generally the OKT8, cells were predominant. The Leu 3A/OKT8 cell ratio was not related to histological type, tumor size, age of the patient or presence of metastases. Some of the T cells had the la antigen and were thus probably activated. The B cells were either absent or less numerous than the T cells. There was no relation between their distribution and the various parameters studied. A few monocytes were heterogeneous according to their markers (OKM I and acid phosphatase). In 6 cases only there was a strong infiltration of mononuclear cells positive for acid phosphatase. The number of the natural killer cells was also low. Only a few mononuclear infiltrating cells had receptors for transferrin. There was a positive correlation between the inflammatory infiltration and the presence of HLA class‐1 antigens on tumor cells. Some of the antisera specific for lymphocyte subsets also stained the breast carcinoma cells. The great variations in the subsets of mononuclear cells in breast carcinomas may correspond to various systems of defense against neoplasm.
Monoclonal‐antibody‐defined human lung tumour cell‐surface antigens
Authors: Reeve Julie G. , Wulfrank Denis A. , Stewart Jonathan , Twentyman Peter R. , Baillie‐Johnson Hugo , Bleehen Norman M. 
Published: February 2007

AbstractA panel of 3 monoclonal antibodies (MAbs) directed against human lung tumour cell‐surface antigens has been produced following immunizations with the established small‐cell lung cancer (SCLC) cell line, NCl‐H69, and with another SCLC cell line, COR‐L32, recently derived from clinical material. One MAb, B10/12, reacted strongly with SCLC, immunoprecipitated a protein having an MW of 100kd and failed to react significantly with non‐small‐cell lung cancer (NSCLC) in radioimmunoassay and in an immunohistochemical assay. MAbs E10/5 and 2G3 reacted extensively with SCLC but also showed significant reactivity with NSCLC. MAb E10/5 immunoprecipitated a protein with an MW of 80kd but no appreciable protein was specifically precipitated by MAb 2G3. Unlike MAb 2G3, both MAbs B10/12 and E10/5 reacted strongly with selected neuroblastomas whereas only MAbs 2G3 and E10/5 reacted significantly with melanoma. All 3 MAbs reacted with breast carcinomas. Other non‐pulmonary tumours thus far examined failed to react with the MAbs in radioimmunoassay or immunohistochemical assay. Immunocytochemistry and the use of viable cells in radioimmunoassay confirmed that the antigenic determinants recognized by these MAbs were surface located.
In vitro cisplatin effects on phagocyte functions
Authors: Fumarulo Ruggiero , Riccardi Silvana , Pantaleo Romeo , Coluccia Mauro , Giordano Domenico , Aresta Michele 
Published: February 2007

AbstractThe effects of cis‐diamminedichloroplatinum (11) (cisplatin), a drug used in cancer chemotherapy, on the oxidative metabolism, endocytosis, chemotaxis and exocytosis of guinea‐pig polymorphonuclear leukocytes were studied. All these functions were negatively influenced, but the same effect (50% inhibition) was observed at different drug concentrations (3 × 10−5 M for chemotaxis, 10−4 M for O2 consumption by FMLP and β‐glucuronidase release, 10−3 M for O2 consumption by PMA and for zymosan engulfment). The effects of the drug can be explained by its ability to bind to membrane proteins, essentially to ‐SH groups.