bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of R213R and 13494 g→a polymorphisms of the p53 gene in individuals with esophagitis, intestinal metaplasia of the cardia and Barrett’s Esophagus compared with a control group
Authors: Pilger Diogo André , Lopez Patrícia Luciana da Costa , Segal Fábio , Leistner-Segal Sandra 
Published: May 2007

Abstract
Protein p53 is the tumor suppressor involved in cell cycle control and apoptosis. There are several polymorphisms reported for p53 which can affect important regions involved in protein tumor suppressor activity. Amongst the polymorphisms described, R213R and 13949 g→a are rarely studied, with an estimate frequency not yet available for the Brazilian population. The purpose of this study was to investigate the genotype and allele frequencies and associations of these polymorphisms in a group of patients with altered esophageal tissue from South Brazil and compare with the frequency observed for a control population. A total of 35 patients for R213R and 45 for 13494 g→a polymorphisms analysis with gastroesophageal reflux disease (GERD) symptoms diagnosed by upper digestive endoscopy and confirmed by biopsy were studied. For both groups, 100 controls were used for comparison. Loss of heterozygosity (LOH) was also analyzed for a selected group of patients where normal and affected tissue was available. There was one patient with Barrett’s Esophagus (BE) showing LOH for R213R out of two heterozygous samples analyzed and two patients (esophagitis and BE) for 13494 g→a polymorphism. We also aimed to build a haplotype for both polymorphisms collectively analyzed with R27P polymorphism, previously reported by our group. There were no significant differences in allele and genotype distribution between patients and controls. Although using esophagitis, intestinal metaplasia of the cardia and BE samples, all non-neoplastic lesions, we can conclude that these sites do not represent genetic susceptibility markers for the development and early progression of GERD to BE and esophageal cancer. Additional studies are required in order to investigate other determiners of early premalignant lesions known to predispose to esophageal cancer.
Tensile, Compression and Fracture Properties of Thick-Walled Ductile Cast Iron Components
Authors: Minnebo P. , Nilsson K.-F. , Blagoeva D. 
Published: May 2007

Abstract
The article presents the outcome of a comprehensive program of tensile, compression and fracture toughness experiments, addressing thick-walled ductile cast iron inserts used for the production of three nuclear waste canisters. The resulting data are required as input to the assessment of the failure probability of the canisters. Moreover, these data are useful for the improvement of the casting technique as such. Although the same material specification is always used, material properties are found to show significant variation. Considerable attention is paid to linking the scatter in tensile properties to fractographic and microstructural observations. The main finding is that low ductility tensile test results can be primarily connected to the presence of specific casting defects, from which oxide films have the most detrimental effect. Another important observation is that compression experiments do not result in low ductility failure. During fracture testing, stable ductile crack propagation is observed. Basic fracture analysis of a tensile test is performed in order to better understand the effect of defect size, stress-strain behavior and fracture toughness on the ductility measured through tensile testing. Two opposing specimen size effects are observed.
A Headspace–SPME–MS Method for Monitoring Rapeseed Oil Autoxidation
Authors: Jeleń H. H. , Mildner‐Szkudlarz S. , Jasińska I. , Wąsowicz E. 
Published: May 2007

AbstractHeadspace SPME–MS was used to analyze volatile compounds from rapeseed oil subjected to an accelerated storage test consisting of 0–12 days of storage at 60 °C. The SPME–MS data was compared with the data obtained by solid phase microextraction–gas chromatography/mass spectrometry (SPME–GC/MS). The SPME–GC/MS method allowed detection of 37 volatile compounds, of which 28 were identified. Predominant ones were hexanal, 2,4‐heptadienal, 2‐heptenal and 1‐pentene‐3‐ol. Volatile compounds were not separated in SPME–MS—a single peak reflecting the total amount of volatiles was obtained. An increase in the abundance of characteristic ions in this peak could be used to detect of compounds characteristic for rapeseed oil autoxidation. These compounds (with their characteristic ions) were hexanal (m/z 56), 1‐pentene‐3‐ol and 1‐octene‐3‐ol (m/z 57), 2‐pentenal and 2‐heptenal (m/z 83 and 84), and 2,4‐heptadienal (ions m/z 81 and 110). The SPME–MS peak area was correlated with peroxide value at 0.9779 and with Totox at 0.9841. Principal component analysis (PCA) of fatty acid volatile oxidation products from a model rapeseed oil indicated that SPME–MS was able to differentiate samples containing hexanal at a concentration of 0.2, 0.4, 0.6, 0.8 and 1.0 mg/L with proportional amounts of other compounds. Further, samples that were subjected to 0, 2, 4, 6, 8, 10 and 12 days of storage at 60 °C were differentiated using SPME–MS–PCA. PCA showed similarities in clustering of the data obtained by SPME–MS and sensory analysis.
Evidence for a 55–50 ka (early Wisconsin) glaciation of the Cordilleran ice sheet, Yukon Territory, Canada
Authors: Ward Brent C. , Bond Jeffrey D. , Gosse John C. 
Published: May 2007

AbstractCosmogenic 10Be ages on boulders of 54–51 ka (n=4) on a penultimate Cordilleran ice sheet (CIS) drift confirm that Marine Oxygen Isotope Stage (MIS) 4 (early Wisconsin) glaciation was extensive in parts of Yukon Territory, the first confirmed evidence in the Canadian Cordillera. We name the glaciation inferred from the mapped and dated drift the Gladstone. These results are in apparent contrast to the MIS 6 (Illinoian) age of the penultimate Reid glaciation to the east in central Yukon but are equivalent to exposure ages on MIS 4 drift in Alaska. Contrasting penultimate ice extents in Yukon requires that different source areas of the northern CIS in Yukon responded differently to climatic forcing during glaciations. The variation in glacier extent for different source areas likely relates to variation in precipitation during glaciation, as the northern CIS was a precipitation-limited system. Causes for a variation in precipitation remain unclear but likely involve the style of precipitation delivery over the St. Elias Mountains possibly related to variations in the Aleutian low.
Dealcoholized red and white wines decrease oxidative stress associated with inflammation in rats
Authors: López D. , Pavelkova M. , Gallova L. , Simonetti P. , Gardana C. , Lojek A. , Loaiza R. , Mitjavila M. T. 
Published: May 2007

In vitroexperiments have demonstrated that polyphenols exhibit antioxidant and anti-inflammatory activities. The present study was designed to test whether dealcoholized red (DRW) and white (DWW) wines can decrease the oxidative stress associated with inflammationin vivo. Rats were fed for 15 d either a control diet or one supplemented with DRW or DWW. Finally, a granuloma was induced by subcutaneous administration of carrageenan. Although DRW showed higher antioxidant activityin vitrothan DWW, both wines decreased the number of cells recruited into the granuloma pouch. Malondialdehyde decreased in plasma and inflammatory exudate from rats fed with DRW- and DWW-rich diets. Moreover, the concentration of NO increased in exudate, which correlates with the increase in the citrulline:arginine ratio. Polymorphonuclear leucocytes from the inflammatory exudate of rats fed dealcoholized wines showed decreased superoxide anion (O2∙−) production and increased NO productionex vivo. This change in NO production resulted from increased expression and activity of inducible NO synthase (EC 1.14.13.39). Moreover, the up regulation of cyclo-oxygenase-2 (EC 1.14.99.1) protein expression observed in rats fed the DRW-rich diet was not related to a direct effect of NO. The present results indicate that the non-alcoholic compounds of wines not only improve antioxidant status in an inflammatory situation, but also limit cell infiltration, possibly through a decrease in O2∙−and an increase in NO production.
Genetic variation among Mediterranean populations ofSesamia nonagrioides(Lepidoptera: Noctuidae) as revealed by RFLP mtDNA analysis
Authors: Margaritopoulos J.T. , Gotosopoulos B. , Mamuris Z. , Skouras P.J. , Voudouris K.C. , Bacandritsos N. , Fantinou A.A. , Tsitsipis J.A. 
Published: May 2007

AbstractRestriction fragment length polymorphism analysis of two segments of mitochondrial DNA (COI and 16S rRNA) was used to examine genetic variation inSesamia nonagrioides(Lefèbvre) populations from the Mediterranean basin. Four populations were collected from central and southern Greece, and five from northern latitudes: Greece, Italy, France and Spain. No variation was observed in COI, while 16S rRNA segment proved highly polymorphic and 28 different haplotypes were found. Lower intra-population polymorphism was found in the northern populations than in southern ones. Although no significant isolation by distance was found, the UPGMA tree based on Nei's raw number of nucleotide differences separated the populations into two major groups, i.e. one with the northern (40.6°N–43.4°N) and the other with the southern populations (37.3°N–39.2°N). Analysis of molecular variance revealed that most of the variation was between the two major groups (ΦCT=0.559), and all pairwise comparisons between the northern and southern populations resulted in high and significantFSTvalues (overallFST=0.604). The highFSTvalues and the strong spatial genetic structure indicate that long-distance migration may be a rare event. The populations do not seem to have experienced a strong historical bottleneck. The occurrence of a few widespread haplotypes and the genetic similarity of the northern populations could be attributed to a historical expansion of certain haplotypes from the south towards to the northern borders of the species' distribution area.
Recurrent motions within plane Couette turbulence
Authors: VISWANATH D. 
Published: May 2007

The phenomenon of bursting, in which streaks in turbulent boundary layers oscillate and then eject low-speed fluid away from the wall, has been studied experimentally, theoretically and computationally for more than 50 years because of its importance to the three-dimensional structure of turbulent boundary layers. Five new three-dimensional solutions of turbulent plane Couette flow are produced, one of which is periodic while the other four are relative periodic. Each of these five solutions demonstrates the breakup and re-formation of near-wall coherent structures. Four of our solutions are periodic, but with drifts in the streamwise direction. More surprisingly, two of our solutions are periodic, but with drifts in the spanwise direction, a possibility that does not seem to have been considered in the literature. It is argued that a considerable part of the streakiness observed experimentally in the near-wall region could be due to spanwise drifts that accompany the breakup and re-formation of coherent structures. A new periodic solution of plane Couette flow is also computed that could be related to transition to turbulence.The violent nature of the bursting phenomenon implies the need for good resolution in the computation of periodic and relative periodic solutions within turbulent shear flows. This computationally demanding requirement is addressed with a new algorithm for computing relative periodic solutions one of whose features is a combination of two well-known ideas – namely the Newton–Krylov iteration and the locally constrained optimal hook step. Each of the six solutions is accompanied by an error estimate.Dynamical principles are discussed that suggest that the bursting phenomenon, and more generally fluid turbulence, can be understood in terms of periodic and relative periodic solutions of the Navier–Stokes equation.
Thelidium rimosulum (Verrucariaceae, lichenized Ascomycota), a new lichen species from Poland
Authors: CEYNOWA-GIEŁDON Mirosława 
Published: May 2007

Abstract:%Thelidium rimosulum sp. nov. is described from limestone in an industrialized area near Piechcin, north-central Poland (Kujawy). The species is mainly characterized by the small, bicellular ascospores, a well-developed involucrellum and a thin, often isidiate or sorediate, thallus.
Gorillas in the midst: the impact of armed conflict on the conservation of protected areas in sub-Saharan Africa
Authors: Glew L. , Hudson M.D. 
Published: May 2007

AbstractThe frequently anecdotal nature of evidence concerning the impact of warfare on conservation poses numerous problems and there have been calls to apply a strict set of conditions to such data to improve the rigor of scientific analysis in this field. To illustrate the difficulties, however, of applying strict quantitative conditions on such data a deterministic model of conflict-linked deforestation in sub-Saharan Africa was constructed and the implications of the model discussed. Our model indicates that from 1990–2005 approximately 35,000 ha of timber have been used to support officially recorded UN refugees in this region: this is a continuing impact, albeit quantified using data with some potential error. An alternative semi-quantitative approach was also used, with reported environmental impacts of conflict assessed for reliability and severity using a number of empirical criteria. Data focusing on the Democratic Republic of Congo and Rwanda were subsequently analysed using this framework. Illegal resource exploitation was identified as the primary impact resulting from conflict and, in some instances, a driver of the hostilities. From the joint consideration of the conflict and post-conflict phases such exploitation is concluded to be the product of lawlessness and anarchy generated by violent uprisings rather than violence per se. As such, armed conflict does not pose a novel threat to protected areas but rather amplifies threats extant during peace, creating a need for appropriate responses by those involved in conservation management. With both the occurrence and violence of conflicts in sub-Saharan Africa increasing, the impacts of warfare are pertinent to both the immediate and long-term management of biological resources in the region.
The reintroduction of large carnivores to the Eastern Cape, South Africa: an assessment
Authors: Hayward Matt W. , Kerley Graham I. H. , Adendorff John , Moolman Lucius C. , O'Brien John , Sholto-Douglas Angus , Bissett Charlene , Bean Peter , Fogarty Alan , Howarth Dale , Slater Richard 
Published: May 2007

AbstractRecently, conservation estate in South Africa's Eastern Cape Province has increased 10-fold resulting in large predators being increasingly reintroduced to restore ecological integrity and maximize tourism. We describe the reintroductions of large carnivores (>10 kg) that have occurred in the Eastern Cape and use various criteria to assess their success. Lion Panthera leo reintroduction has been highly successful with a population of 56 currently extant in the region and problems of overpopulation arising. The African wild dog Lycaon pictus population has increased to 24 from a founder population of 11. Preliminary results for spotted hyaenas Crocuta crocuta also indicate success. Wild populations of leopards Panthera pardus exist on several reserves and have been supplemented by translocated individuals, although deaths of known individuals have occurred and no estimate of reproduction is available. Cheetah Acinonyx jubatus reintroduction has also been less successful with 36 individuals reintroduced and 23 cubs being born but only 41 individuals surviving in 2005. Criteria for assessing the success of reintroductions of species that naturally occur in low densities, such as top predators, generally have limited value. Carrying capacity for large predators is unknown and continued monitoring and intensive management will be necessary in enclosed, and possibly all, conservation areas in the Eastern Cape to ensure conservation success.
Assessing the failure of a community-based human-wildlife conflict mitigation project in Budongo Forest Reserve, Uganda
Authors: Webber A. D. , Hill C. M. , Reynolds V. 
Published: May 2007

AbstractPrimate crop raiding is a major cause of human-wildlife conflict around the forests of western Uganda. In an attempt to ameliorate the situation a conflict mitigation strategy was established in villages around the Budongo Forest Reserve in 2001. Live-traps were constructed that allowed the identification of crop raiding animals; pest species could be disposed of and threatened species released unharmed. However, by 2004 none of the traps in the study area were functioning and interviews were conducted to assess the reasons for their decline and local people's acceptance of the intervention. Forty-one percent of respondents did not believe the strategy was effective and the majority of local farmers did not accept responsibility for the traps. This was because of operational failures in four areas: (1) the identification of key stakeholders, (2) objective evaluation to assess the efficacy and benefit of the intervention, (3) participatory monitoring and evaluation, and (4) long-term funding commitment by conservation agencies. We examine the impact of these four elements upon the sustainability of the live-trap programme and stress the importance of recognizing and reporting failures to develop effective and acceptable mitigation strategies.
Nesting and at-sea distribution of marine turtles in Angola, West
Africa, 2000–2006: occurrence, threats and conservation implications
Authors: Weir Caroline R. , Ron Tamar , Morais Miguel , Duarte Agostinho Domingos C. 
Published: May 2007

AbstractThe status of marine turtles in Angola, West Africa, is poorly known, and
therefore during 2000–2006 a combination of both dedicated and
opportunistic beach and at-sea turtle surveys were carried out, and interviews
conducted with fishing communities and at markets. Green Chelonia mydas, olive ridley Lepidochelys olivacea, leatherback Dermochelys coriacea and loggerhead turtles Caretta caretta were recorded, and nesting of the
first three species confirmed during September–March (peaking
November–December). Green turtles nested mainly in the south,
leatherback turtles in north and central Angola, and olive ridley turtle nesting
was widespread. Olive ridley turtle nest density at Palmeirinhas averaged 32
nests km−1. At-sea surveys produced 298 turtle records,
with peak occurrence during August. Significant anthropogenic-related mortality
(including exploitation of meat and eggs and fishing bycatch) was recorded, in
addition to natural predation and other threats. Maintenance of the long-term
sustainability of these turtle populations should focus on the involvement of
fishing communities and increasing awareness throughout Angola.
Paeoniflorin inhibits TGF-β1-mediated collagen production bySchistosoma japonicumsoluble egg antigenin vitro
Authors: CHU D. , LUO Q. , LI C. , GAO Y. , YU L. , WEI W. , WU Q. , SHEN J. 
Published: May 2007

SUMMARYThe main pathological characteristics of hepatic fibrosis in schistosomiasis are the proliferation of hepatic stellate cells (HSCs) and the deposition of collagen type I (Col I) and collagen type III (Col III). Transforming growth factor beta-1 (TGF-β1) plays an important role in hepatic fibrosis. Paeoniflorin (PAE) has been reported to have immunoregulatory effects; however, the mechanism of its anti-hepatic fibrosis inS. japonicumhas not been elucidated. In the present study, we found that mouse peritoneal macrophages (PMφs) stimulated by soluble egg antigen (SEA) ofS. japonicumcould secrete TGF-β1, and the TGF-β1 in the peritoneal macrophage-conditioned medium (PMCM) could induce proliferation of HSCs and secretion of Col I and III. We selected PMCM at 1:2 dilution as the optimum PMCM (OPMCM). Then we treated HSCs pre-incubated with OPMCM with PAE, and found that the inhibition of HSC proliferation or Col I and III production were closely correlated with the concentration of PAE. Further investigation found that PAE significantly decreased the Smad3 transcription and phosphorylation in HSCs stimulated by OPMCM. In conclusion, SEA plays a key role in hepatic fibrosis by inducing TGF-β1 from PMφs. PAE can exert anti-fibrogenic effects by inhibiting HSCs proliferation and down-regulating Smad3 expression and phosphorylation through TGF-β1 signalling.
Polymerase chain reaction in the detection of an ‘outbreak’ of asymptomatic viral infections in a community birth cohort in south India
Authors: GLADSTONE B. P. , ITURRIZA-GOMARA M. , RAMANI S. , MONICA B. , BANERJEE I. , BROWN D. W. , GRAY J. J. , MULIYIL J. , KANG G. 
Published: May 2007

SUMMARYAsymptomatic enteric infections are important where sequelae or protection from subsequent illness is an outcome measure. The use of reverse transcription–polymerase chain reaction (RT–PCR) to identify asymptomatic enteric infections in a birth cohort followed for rotaviral infections in a south Indian urban slum is reported. Of 1191 non-diarrhoeal samples from 371 children collected in May–June 2003, 22 (1·9%) were positive by ELISA. A total of 147 (40·6%) of 362 samples tested by VP6 RT–PCR were positive. In those samples that could be typed, a high diversity of G types including G1, G2, G4, G8, G9 and G10, and a high proportion (34·4%) of mixed infections were detected. Noroviruses were identified in 6/28 (21·4%) samples tested. The identification of infections undetectable by conventional techniques indicates the importance of the use of sensitive diagnostic techniques in research studies. Asymptomatically infected children may also act as a source of infection for other susceptible hosts.
Theoretical modeling of convection I. Key physical processes
Authors: Canuto V. M. 
Published: May 2007

AbstractIf one strives for a reliable description of “Turbulent Mixing in Stars” one must account for a large variety of physical processes. These include non-locality, that is needed in unstably stratified regimes, overshooting, which occurs in a stably stratified regime, double-diffusion processes (semi-convection and salt-fingers), transport of angular momentum, the Li7problem, compressibility, and magnetic fields. While phenomenological models are manifestly inadequate, LES are too computer intensive to tackle this large variety of processes. Since the requirement of completeness of the description of these processes must also result in models that are usable in stellar structure-evolution codes, we conclude that only the RSM (Reynolds Stress Model) can do so and a description of the state of the art in that field is presented in Part 2.
Theories of convection and the spectrum of turbulence in the solar photosphere
Authors: Rincon François 
Published: May 2007

AbstractClassical theories of turbulence do not describe accurately inertial range scaling laws in turbulent convection and notably fail to model the shape of the turbulent spectrum of solar photospheric convection. To understand these discrepancies, a detailed study of scale-by-scale budgets in turbulent Rayleigh-Bénard convection is presented, with particular emphasis placed on anisotropy and inhomogeneity. A generalized Kolmogorov equation applying to convection is derived and its various terms are computed using numerical simulations of turbulent Boussinesq convection. The analysis of the isotropic part of the equation shows that the third-order velocity structure function is significantly affected by buoyancy forcing and large-scale inhomogeneities. Anisotropic contributions to this equation are also shown to be comparable to their isotropic counterpart at moderate to large scales. Implications of these results for convection in the solar photosphere, mesogranulation and supergranulation are discussed.