bluuebunny/crossref_metadata_2025_split huggingface dataset:
DIE INITIALE VERTEILUNG VON RADIO-C IN DEN ZELLFRAKTIONEN EINIGER RATTENORGANE NACH INJEKTION VON TESTOSTERON-4-14C
Authors: Mosebach K.-O. , Dirscherl W. 
Published: December 2008

ABSTRACT
The initial distribution of radioactive C was studied in the cell fractions of the liver, kidney, testes and thigh muscles after intraperitoneal injection of testosterone-4-14C into 40 day old male rats. To make this possible, the absolute and specific activity values (μc/mg C) were determined.
After both ten and twenty minutes the cytoplasm fractions possessed the highest activity values, the only exception being the specific activity of the liver cytoplasm ten minutes after injection when the microsomes of the liver showed a higher activity. After 20 min the mitochondria possessed the highest specific activity values among the corpuscular fractions. The specific activity values in the microsomes of all four organs studied were lower 20 min after the time of injection than after 10 min, a fact, which is suspected to be the result of the initial formation of conjugates in the microsomes.
A PRELIMINARY INVESTIGATION OF STEROID EXCRETION IN DEPRESSED PATIENTS BEFORE AND AFTER ELECTRO-CONVULSIVE THERAPY
Authors: Ferguson H. C. , Bartram A. C. G. , Fowlie H. C. , Cathro D. M. , Birchall K. , Mitchell F. L. 
Published: December 2008

ABSTRACT
Using a method dependent upon paper chromatography, the urinary excretion of the individual corticosteroids and the individual 17-oxosteroids has been studied before and after electro-convulsive therapy in five female patients suffering from a depressive illness. The corticosteroids, which are normally associated with stress, were found to show a fall in excretion from abnormally high levels before treatment to normal levels thereafter. The 11-deoxy-17-oxosteroids, on the other hand, showed a low level of excretion prior to treatment which was followed by a rise to normal values in clinical remission. These findings are discussed.
THE EFFECTS OF CORTISOL ON THE INCORPORATION OF GLYCINE CARBON INTO THE NUCLEIC ACIDS OF NORMAL AND MALIGNANT TISSUES
Authors: de Loecker W. 
Published: December 2008

ABSTRACT
Normal and hepatoma bearing rats were treated with cortisol in order to study the effect of adrenal steroids on nucleic acid metabolism of different tissues. Normal liver, precancerous liver, hepatoma nodules and skeletal muscle were used in these experiments.
The incorporation of 14C from glycine-2-14C into RNA and DNA fractions of different tissues was examined.
The incorporation of glycine carbon into RNA of normal and precancerous liver was found to be stimulated by cortisol treatment.
The RNA fraction of hepatoma tissue of non treated animals showed a higher incorporation level than that observed in liver, but incorporation was drastically inhibited by cortisol.
The DNA fraction of hepatoma tissue showed a higher incorporation than the DNA fraction from the other tissues examined, and followed the same incorporation pattern as RNA.
The incorporation of 14C into the nucleic acids of skeletal muscle was not affected by cortisol treatment.
AUTORADIOGRAPHIC DISTRIBUTION STUDIES WITH 14C-CORTISONE AND 14C-CORTISOL
Authors: Hanngren Å , Hansson E. , Sjöstrand S. E. , Ullberg S. 
Published: December 2008

ABSTRACT
The distribution of 14C-labelled cortisone and cortisol was investigated by wholebody autoradiography in mice.
No clear difference in distribution pattern was found between the two substances. Both were rapidly distributed in all tissues. Apart from organs such as liver, kidney and intestines, selective accumulation was observed in bronchial mucosa, ductus epididymis, vas deferens, interstitial cells of testicle and ovary, and epithelium of the yolk sac. Fairly high concentrations were found in brown fat and in pituitary gland. Both radioactive substances penetrated the placental barrier, but uptake was lower in foetuses than in maternal tissues. The adrenal cortex was the only organ in foetuses to show accumulation. Very little activity was seen in adrenal cortex of the dams. Radioactivity in joints (cartilage and synovial fluid) was low. The sclera, iris and superficial cell layers of the lens showed accumulation of activity. The radioactive substances were seen in choroid plexus and in cerebrospinal fluid. They were evenly but very scantily distributed in brain tissue. The formation and distribution of metabolites are discussed.
ATYPICAL HYPOPARATHYROIDISM
Authors: King Lionel R. , Goldsmith Richard E. 
Published: December 2008

ABSTRACT
Three patients with atypical, intermittent tetany of recent origin, hypocalcaemia, hypocalciuria and normal serum inorganic phosphorus concentration are described. Two patients were postoperative from thyroidectomy by more than 20 years and one had recently experienced metastatic malignant invasion of the thyroid area. A variety of studies showed that these patients were neither normal nor typically hypoparathyroid in the laboratory. Two hypotheses, each with some support are: 1. that the patients were not totally aparathyreotic, and 2. that, while aparathyreotic, they had adapted to the abnormal state by some mechanism operative in the absence of parathyroid hormone.
THE PERCHLORATE TEST IN JUVENILE AUTO-IMMUNE THYROIDITIS
Authors: Nilsson Lars R. , Berne Eric 
Published: December 2008

ABSTRACT
Using the perchlorate test an iodine binding defect in the thyroid was demonstrated in 12 of 25 patients with juvenile auto-immune thyroiditis (aged 7–17 years). Six of the 7 patients with a fall of more than 10 per cent in activity following administration of perchlorate were definitely hypothyroid. When the perchlorate test was positive (> 5 per cent fall) the iodine uptake tended to be low and the protein-bound radioactivity high in conventional 131I tests.
The iodine binding defect underlying the abnormal perchlorate response is probably the result of a progressive thyroid disorder which impairs the iodine binding process more than the iodide concentrating mechanism.
IMMUNOLOGICAL STUDIES ON THE SEX-DEPENDENT PREALBUMIN IN MOUSE URINE AND ON ITS OCCURRENCE IN THE SERUM
Authors: Rümke Ph. , Thung P. ]. 
Published: December 2008

ABSTRACT
Using an antiserum against a protein, which is electrophoretically a pre-albumin and which has previously been described as occurring specifically in mouse urine, it has been found that this protein occurs in the serum of both male and female mice of various strains, its concentration in the serum being about 200 to 400 times lower than in the urine. In the serum, as in the urine, the concentration of this protein is higher in adult male and testosterone-treated female mice than in infantile male or untreated female mice. In the serum, the values found in these two categories are of the order of 50 to 150 and 10 to 20 μg/ml respectively. Our data indicate that male sex hormone stimulates the production of this protein, which probably originates in the liver and is concentrated and excreted by the kidneys.
THE ACTION OF FOLLICLE STIMULATING HORMONE AND OF HUMAN CHORIONIC GONADOTROPHIN UPON STEROID SYNTHESIS BY RABBIT OVARIAN TISSUES IN VITRO
Authors: Gospodarowicz Denis 
Published: December 2008

ABSTRACT
Rabbit ovarian follicles have been incubated with sodium acetate-14C with and without the presence of gonadotrophin. The major radioactive metabolites found were dehydroepiandrosterone (DHEA), androst-5-ene-3β,17β-diol, testosterone, and 17α-oestradiol. Lesser amounts of radioactivity were found in pregnenolone, progesterone, androstenedione, and oestrone. The results are interpreted as evidence that two metabolic pathways exist in the follicle; the major one giving rise to androgens and oestrogens from Δ5-3β-hydroxysteroids while the other pathway involves pregnenolone and progesterone as intermediates. Follicle stimulating hormone (FSH) and human chorionic gonadotrophin (HCG) both increased the incorporation of acetate-14C into steroids and decreased the specific activity of cholesterol. The site of action of the gonadotrophins in the metabolic sequence is discussed.
THE IN VITRO PRODUCTION OF ANDROGENS BY FOLLICULAR TISSUE OF RABBITS
Authors: Gospodarowicz Denis 
Published: December 2008

ABSTRACT
Incubation in vitro of rabbit follicles in separate experiments with dehydroepiandrosterone-14C (DHEA-14C), progesterone-14C and pregnenolone-3H in the presence of FSH gave the following results: 39 % of the radioactivity of DHEA-14C is converted to androstenedione and testosterone, while only 3 % of the radioactivity of either progesterone-14C or pregnenolone-3H is found in the androgen fraction. From the ratio of testosterone to androstenedione formed from the three precursors, the results are interpreted to mean that DHEA and pregnenolone, and not progesterone, are precursors of androgens in the follicle.
TEMPORAL ASPECTS OF DECIDUAL CELL REACTION
Authors: von Berswordt-Wallrabe Ilse , Geller H.-F. , Herlyn U. 
Published: December 2008

ABSTRACT
Temporal aspects of the decidual cell reaction (DCR) were studied in 331 pseudopregnant (lactogenic hormone treated) rats under rhythmical and permanent illumination. The peak of uterine sensitivity to the trauma in rats, exposed to light for 14 hours/day was found at 2 p. m. on day 4, and in rats, exposed to permanent illumination at 6 a. m. on day 4 of pseudopregnancy. Rats, traumatized during the decreasing phase of sensitivity, only showed the DCR restricted adjacent to the cervix. Standardized light exposure is postulated for studies on the DCR.
SYNTHETIC ACTIVITY IN MOUSE ORGANS MAINTAINED ON CHEMICALLY-DEFINED MEDIA.
Authors: Lostroh Ardis J. 
Published: December 2008

ABSTRACT
Atrophic uteri taken from ovariectomized C57 B1 mice hypertrophy when maintained as organ cultures in an atmosphere of 5 % CO2 – 95 % O2 on a medium made up of 4 parts Waymouth's-199 synthetic medium and 1 part agar. Cells of both the endometrium and myometrium respond. Net amounts of protein are synthesized by the explants; after 6 days of culture, the net increment of protein was 60 % on the basal medium alone and 80 % when insulin was included.
An effect of insulin on the specific radioactivity of uterine protein was evident as early as 8 hours after the explants had been placed on the culture medium that contained leucine-1-14C. The differences between the control and insulin groups were progressively accentuated over the course of the first 5 days of culture, but were not always apparent after as long as 6 days.
THE ANTIGENIC PROPERTIES OF PIG INSULIN
Authors: Brunfeldt K. , Deckert T. 
Published: December 2008

ABSTRACT
In the present study rabbits were immunized with insulin preparations of various degrees of purity. Antibody formation was determined by Boyden's haemagglutination method (Boyden 1951), by Berson et al.'s chromatoelectrophoresis (Berson et al. 1956) and by agar electrophoresis In the two last mentioned methods highly purified 131I-pig insulin was used. The agglutination method gave a positive reaction with sera from rabbits immunized with the two least purified preparations, A-insulin and B-insulin, thus suggesting the formation of antibodies against impurities in these two preparations. On the other hand, agar electrophoresis, and to a lesser degree chromatoelectrophoresis, gave positive reactions with sera from all groups. Thus it was shown that antibodies were also formed in rabbits immunized with even the most purified insulin preparation. Antibody formation was, however, most pronounced in those rabbits which had been vaccinated with the most impure insulin preparation.
By continuous paper electrophoresis the purest of the insulin preparations used for immunization could be separated into two fractions. The slowest migrating fraction tested by the haemagglutination test showed a weak but positive reaction indicating traces of impurities.
Thus, although the possibility of a cross reactivity between insulin and the impurities demonstrated cannot be rejected, the results obtained do not allow of any definite decision on this question. The negative inhibition with the purest insulin preparation, however, suggests that there is no common antigenicity.
THE POSSIBLE OCCURRENCE OF TWO INBORN ERRORS OF IODINE METABOLISM IN ONE PATIENT
Authors: Wiener J. D. , Lindeboom G. A. 
Published: December 2008

ABSTRACT
The occurrence of two separate inborn errors of iodine metabolism in one patient has been suggested by some authors but doubted by others. The combination of two other mechanisms may explain most of these cases: one defect may be conditioned by another, or the defects may have a common underlying cause. A seemingly impeded deiodination of iodotyrosines, found together with one of the other defects in a hypothyroid patient, may be an example of the first mechanism. The second mechanism might be involved when the primary abnormality is located in the biogenesis of thyroglobulin. In the absence of normal thyroglobulin, three successive reactions could secondarily be influenced: the iodination, the »coupling« and the proteolysis. Thus, when two or more of these reactions are found to be (partially) blocked, defective thyroglobulin biogenesis should be considered as a possibility.
A case is presented in which such a defect was at first suggested by the simultaneous occurrence of circulating iodinated protein-like compounds (defective proteolysis) and the secretion of large amounts of iodide by the thyroid (hampered coupling), and was subsequently confirmed by the electrophoretic analysis of a thyroid extract which showed an absence of normal thyroglobulin. Furthermore, additional evidence is presented on a similar patient described in a previous paper. Finally, a survey is given of sixteen other patients described in the literature in whom the existence of defective thyroglobulin biogenesis was either suggested by the occurrence of two seemingly separate defects or confirmed by the absence of normal thyroglobulin.
STUDIES ON THE TURN-OVER OF SIMULTANEOUSLY INJECTED GUINEA-PIG ALBUMIN-125I AND THYROXINE-131I IN SKIN, WOUNDS AND LIVER OF GUINEA PIGS
Authors: Moltke Erik , Funch-Rosenberg Hans , Hansen Heinz J. M. 
Published: December 2008

ABSTRACT
The distribution of simultaneously injected 125I-labelled guinea-pig albumin and 131I-labelled thyroxine in the skin, skin-wounds and liver of guinea-pigs has been investigated. In the plasma albumin was retained in larger amounts than thyroxine but the activities decreased from 2–24 hours after the injection (h. p. i.) at exactly the same rate. In the liver the uptake of thyroxine was considerably higher than that of albumin, but both decreased at the same rate as in the plasma. In the skin thyroxine was also taken up in relatively larger amounts than albumin at 2 and 6 h. p. i.; thyroxine decreased at the same rate as in the plasma, while the uptake of albumin increased. In 7-day wounds the thyroxine content was only a little higher than in normal skin at 2 h. p. i. while the albumin content had markedly increased; thyroxine decreased and albumin increased at the same rates as in skin. Fresh wounds showed a very high uptake of both albumin and thyroxine with no decrease from 2–24 h. p. i.; the relation between the amounts was close to – but significantly different from – that seen in plasma.
The results clearly indicate that albumin and thyroxine move quite independently of each other in skin, old skin-wounds and liver. In fresh wounds, however, there is a constant thyroxine-albumin ratio during the experimental period.
DECREASED EFFECTIVENESS OF ALDOSTERONE ON ACTIVE SODIUM TRANSPORT BY THE ISOLATED TOAD BLADDER IN THE PRESENCE OF OTHER STEROIDS
Authors: Crabbé Jean 
Published: December 2008

ABSTRACT
Aldosterone can stimulate the active transport of sodium by the isolated toad bladder in vitro. Corticosterone, cortisol, 17β-oestradiol and progesterone had no such effect despite incubation of the preparation with more than 100 times the smallest effective concentration of aldosterone. When the serosal surface of the membranes was exposed to 10 μg% d-aldosterone combined with 100 times this concentration of corticosterone, cortisol or progesterone, the stimulating action of aldosterone was reduced. Spirolactone SC 9420 failed to exert by itself a significant effect on the isolated toad bladder in vitro, but this compound blocked the stimulation by aldosterone of active sodium transport in vitro when present at concentrations 50 times those of aldosterone. When the concentration ratio was 10:1, a hormonal effect could be demonstrated, except at the highest concentration of the drug used.
COMBINED MALIGNANT EXOPHTHALMOS OF GRAVES' DISEASE AND DIABETIC RETINOPATHY, TREATED BY HYPOPHYSEAL STALK SECTION
Authors: Schlesinger F. G. , Schwarz F. , der Kinderen P. J. , Parren H. G. , Verbiest H. 
Published: December 2008

ABSTRACT
Graves' disease developed in a 45 years old male patient suffering from long standing diabetes mellitus with mild retinopathy.
During a period of overtreatment with antithyroid drugs and following psychological stress, progressive oedematous exophthalmos developed which caused an unusual and very alarming ophthalmological picture. Initially there was a good response to 131I and corticosteroid therapy, but later on pituitary stalk section had to be done, after which the condition of the eyes improved.
The clinical picture was interpreted as the result of Graves' disease in a patient whose vessels were already damaged by diabetic retinopathy.
Serum EPS activity was elevated before the operation and could not be demonstrated postoperatively.
A CLINICAL BIOASSAY METHOD OF LONG-ACTING CORTICOTROPHIN PREPARATIONS AND ITS APPLICATION AS AN INTRAMUSCULAR CORTICOTROPHIN TEST
Authors: Colldahl H. , Diczfalusy E. 
Published: December 2008

ABSTRACT
The corticosteroidogenetic potency of a corticotrophin preparation – given as an intravenous infusion – was compared in asthmatic patients with that of the same preparation dissolved in an aqueous polyphloretin phosphate solution and given as a single intramuscular injection. Four-point assays with a completely randomized block design were employed, each patient being injected with each of the four doses in a different order.
The results indicate that for the purposes of a clinical corticotrophin (ACTH)-test, the 8-hours' intravenous infusion of corticotrophin can suitably be replaced by a single intramuscular injection of a mixture of corticotrophin and polyphloretin phosphate.
The clinical bioassay described appears also suitable for the assessment of the corticosteroidogenetic potency of various types of long-acting corticotrophin preparations.
CATABOLISM OF 14C-HISTAMINE IN ADRENOCORTICAL INSUFFICIENCY IN MAN
Authors: Falkheden Thomas , Lindell Sven-Eric , Westling Håkan 
Published: December 2008

ABSTRACT
The urinary excretion of 14C-histamine and its metabolites was studied in four patients following an intravenous injection of 14C-labelled histamine. Two patients were investigated before and after hypophysectomy, which induced laboratory evidence of adrenocortical insufficiency. Two subjects with pituitary insufficiency (gonadal and adrenocortical insufficiency) due to a chromophobe adenoma were studied before and during cortisone therapy.
The presence of adrenocortical insufficiency was associated with a slower rate of elimination into the urine of unchanged 14C-histamine. There was no evidence of a decreased inactivation of 14C-histamine as revealed by analyses of the urinary metabolites.
COMPARATIVE SKIN TESTS WITH ANIMAL AND SYNTHETIC CORTICOTROPHIN IN PATIENTS HYPERSENSITIVE TO ANIMAL CORTICOTROPHIN
Authors: Buytendijk H. J. , Maesen Fr. 
Published: December 2008

ABSTRACT
A study of the literature shows that 5 % of patients are hypersensitive to animal ACTH. Anaphylactic shock, even with a fatal issue, may occur (van Ufford 1952; Hill & Swinburn 1954; Eisalo et al. 1956). The same rather high percentage was also found for our own patients.
In seven patients who had previously shown violent clinical reactions following the use of animal ACTH (Cortrophin), comparative skin tests were carried out with Cortrophin and the synthetic ACTH preparation, tetracosapeptide (CIBA 30.920-Ba).
All patients showed a markedly positive skin reaction to the highly purified animal ACTH (Cortrophin); in two of these patients, the reaction occurred late. Skin reactions to the synthetic ACTH were negative. The synthetic ACTH, must be regarded as a valuable addition in the therapeutic use of ACTH, particularly in patients predisposed to allergic reactions (asthmatics).
EFFECT OF LARGE DOSES OF RADIOIODINE ON THYROID FUNCTION
Authors: Jovanović M. , Djurdjević Dj. , Sinadinović J. 
Published: December 2008

ABSTRACT
The effects of relatively high doses of 131I (150 μc/100 g body weight) were observed on the percentage deposition of 131I in the thyroid glands of rats, and on the pattern of histological localization as shown by autoradiograms.
At 24 hours after dosage, there was no interference in the capability of the gland to concentrate iodine and to convert it to the organic form. However, there was a marked inhibition of the transfer of thyroglobulin from the cells to the colloid as shown by the autoradiograms.
The possible use of this behaviour as a method for study of the mechanism of hormone synthesis is illustrated.
EFFECT OF LARGE DOSES OF RADIOIODINE ON THYROID FUNCTION
Authors: Jovanović M. , Djurdjević Dj. , Sinadinović J. 
Published: December 2008

ABSTRACT
The effect was studied of large doses of radioiodine on the relative amounts of various organic iodine compounds present in the thyroid glands of rats. At 24 hours there was no effect of radiation from 131I on the relative amounts of diiodotyrosine, monoiodotyrosine, and thyroxine plus triiodothyronine found in the gland. After 48 hours and 5 days, during which time there is known to be considerable destruction of epithelial cells, there was an inhibition of thyroxine synthesis but not of the iodination of tyrosine. There is evidence that even at some days after irradiation at the levels used some follicles are preserved and able to synthesize hormones.
IDIOPATHIC HYPOPARATHYROIDISM WITH UNUSUAL BONE CHANGES AND SPASTIC PARAPLEGIA
Authors: Gibberd F. B. 
Published: December 2008

ABSTRACT
A patient, aged 32 years, with idiopathic hypoparathyroidism with unusual bone changes and spasticity is described. In addition she had abnormal thyroid function tests and hypertension. Her brother had ankylosing spondylitis.
In the pelvis and lumbar spine there was well marked sclerosis and calcification of the ligaments and tendon insertions. The condition was unlike ankylosing spondylitis in that there was no sclerosis of the sacroiliac joints and in the lumbar spine the anterior longitudinal ligament was not involved. The similarity to a case described by Salvesen & Bøe (1953) is discussed and the suggestion made that the bone changes are a characteristic feature of some cases with hypoparathyroidism.