bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic effects in iron phthalocyanine-like compounds
Authors: Amulevičius Arũnas , Mažeika Kęstutis , Remeikis Vidmantas , Undzėnas Algimantas 
Published: February 2007

Abstract
57Fe isotope-enriched iron phthalocyanine has been synthesized and studied by the Mössbauer spectroscopic method. The samples were investigated in the temperature range 100–350 K. Some peculiarities in atomic and molecular electronic structure fluctuations were observed. The intermediate Fe oxidation states are observed and it has been found that the rate of charge redistribution is temperature-dependent. The correlation between the temperature behavior of the isomeric shift and quadrupole splitting has been demonstrated. At elevated temperatures above 300 K, the Fe2+ oxidation number is quasistable. The alteration of Fe oxidation number is associated with fluctuations of electron charges, and the electron-accepting and electron-donating properties of surrounding ligands. The energy barriers separating Fe oxidation states have been determined. The possibility of modeling Mössbauer spectra of electron-transfer systems has been shown.
Hidden secrets of the Northern Adriatic: “Tegnúe”, peculiar reefs
Authors: Casellato Sandra , Masiero Luciano , Sichirollo Elena , Soresi Stefano 
Published: February 2007

Abstract
Research carried out over the last 40 years has underlined the scientific importance of the rocky outcrops scattered on the Northern Adriatic Sea bed sometimes referred to as “tegnúe”. The zoobenthic biocenoses developing over these peculiar geological formations are as extraordinary as they are unique. A study carried out for an entire year in two sampling stations, at different distances from the coast, revealed a very high number of zoobenthic species, including those which have now become rare and are therefore protected in Italian seas. The water turbidity of the northern Adriatic Sea greatly reduces the quantity of light reaching these outcrops, limiting the activity of autotrophic organisms only to sciaphilous genera. Thus, the most represented trophic categories of zoobenthos are suspension, especially filter feeders. Biodiversity values calculated for the communities of these particular reefs are far higher than normally found in the soft seabed in nearby areas, but even higher than in other coralligenous outcrops in other marine ecosystem in the world. The ecological role played by the tegnúe in the Northern Adriatic is extraordinary because as well as being true oases of biodiversity, they are areas naturally protected against bottom trawl-fishing. Thus, they offer shelter and reproduction sites for a number of fish and invertebrate species, including some under stress due to severe fishing pressure.
Can low concentrations of Papain help repair articular cartilage defects?
Authors: Staponas Alfredas , Gražienë Vida , Leonavičienë Laima 
Published: February 2007

Abstract
In the present study, we investigate the capability of low concentrations of Papain to stimulate cartilage mesenchymal cells proliferation and transformation to chondrocytes and evaluate the healing capability of partial thickness defects in medial condyle cartilage of 30 rabbits’ knee joints. Papain 0.1 mg/ml and Ringer saline l ml each were injected intra-articularly to rabbits of experimental and control groups (15 animals each). Healthy cartilage from lateral condyle and cartilage from medial condyle where the surgical defect was created were studied histologically and by TEM. The study revealed that 0.1 mg/ml Papain activates proliferation and spreading of mesenchymal stem cells to young forms of chondrocyte from perichondrium to the upper layers of healthy cartilage. In only 22.27% cases of the experimental group, surgical defects filled with cartilaginous tissue on the background of distinct destruction of collagenous matrix in the native cartilage. However, in 55.5% of the control group the defect was spontaneously healed by hyaline cartilaginous tissue completely or partially on the basis of slight destruction of collagenous matrix. The defect site was filled with activated chondrocyte-like cells from the subchondral plate (not perichondrium) in both groups, which acquired some cisterns of rough endoplasmic reticulum (RER) and produced matrix proteins. The results suggest that Papain did not ameliorate the recovery of cartilage defects acquired through surgically-induced injury of collagenous matrix in native cartilage. We observed that articular cartilage is the source of mesenchymal stem cells which have the ability to transform into young forms of chondrocytes. This transformation process depends on the level of destruction of native cartilage collagen matrix induced by the defect or by Papain.
Coronary artery anomalies — a short review
Authors: Zemanek David , Veselka Josef 
Published: February 2007

Abstract
Coronary artery anomalies are rare but sometimes important findings in the evaluation of the coronary artery. The majority of the anomalies are of benign prognosis, but others can be associated with cardiac symptoms and syndromes (angina, dyspnea, syncope, congestive heart failure, myocardial infarction and sudden death). The potentially serious anomalies include: ectopic coronary origin from the pulmonary artery; ectopic coronary origin from the opposite aortic sinus; and large coronary fistulae. Appropriate diagnosis is critical for recognition and management. Treatment can be performed by surgical or percutaneous approach. However, management is conservative in the majority of them. Contemporary diagnosis and clinical management of these anomalies are briefly reviewed and discussed in this article.
An autoradiographic study of cellular proliferaton, DNA synthesis and cell cycle variability in the rat liver caused by phenobarbital-induced oxidative stress: The protective role of melatonin
Authors: El-Sokkary Gamal 
Published: February 2007

AbstractThe protective effect of melatonin against phenobarbital-induced oxidative stress in the rat liver was measured based on lipid peroxidation levels (malondialedyde and 4-hydroxyalkenals). Cellular proliferation, DNA synthesis and cell cycle duration were quantitated by the incorporation of 3H-thymidine, detected by autoradiography, into newly synthesized DNA. Two experiments were carried out in this study, each on four equal-sized groups of male rats (control, melatonin [10 mg/kg], phenobabital [20 mg/kg] and phenobarbital plus melatonin). Experiment I was designed to study the proliferative activity and rate of DNA synthesis, and measure the levels of lipid peroxidation, while experiment II was for cell cycle time determination. Relative to the controls, the phenobarbital-treated rats showed a significant increase (P < 0.01) in the lipid peroxidation levels (30.7%), labelling index (69.4%) and rate of DNA synthesis (37.8%), and a decrease in the cell cycle time. Administering melatonin to the phenobarbital-treated rats significantly reduced (P < 0.01) the lipid peroxidation levels (23.5%), labelling index (38.2%) and rate of DNA synthesis (29.0%), and increased the cell cycle time. These results seem to indicate that the stimulatory effect of phenobarbital on the oxidized lipids, proliferative activity, kinetics of DNA synthesis and cell cycle time alteration in the liver may be one of the mechanisms by which the non-genotoxic mitogen induces its carcinogenic action. Furthermore, melatonin displayed powerful protection against the toxic effect of phenobarbital.
Post-transcriptional modifications of VEGF-A mRNA in non-ischemic dilated cardiomyopathy
Authors: Kowalczyk Jacek , Domal-Kwiatkowska Dorota , Mazurek Urszula , Zembala Michał , Michalski Bogdan , Zembala Marian 
Published: February 2007

AbstractVascular endothelial growth factor (VEGF-A) is one of the most important proangiogenic factors. It has many isoforms encoded by one gene. The occurrence of these isoforms is associated with the process of alternative splicing of mRNA. Some of the splice forms are perceived as tissue specific. The aim of this study was to determine the alternative splicing of VEGF-A mRNA in dilated cardiomyopathy, especially at the level of particular myocardial layers. The assessment of post-transcriptional modifications of VEGF-A mRNA was made on specimens taken from the explanted hearts of patients undergoing cardiac transplantation. Molecular and histopathological studies were perfomed on particular layers of the myocardial muscle (endocardium, myocardium, epicardium). A molecular analysis of cardiac samples was performed by quantitative analysis of the mRNA of the studied VEGF-A isoforms (VEGF121, -145, -165, -183, -189, and -206) using QRTPCR with an ABI-PRISM 7700-TaqMan sequence detector. 72 cardiac specimens taken from the explanted hearts were analyzed. Each of the studied VEGF-A splice forms was present in the evaluated hearts, but the types of alternative splicing of mRNA were different in particular layers. Quantitative analysis revealed different amounts of the studied isoforms. Generally, significantly increased expression of the VEGF-A isoforms was observed in samples taken from hearts with post-inflammatory etiology of cardiomyopathy. Our conclusions are: 1. All the studied VEGF-A isoforms were found in the human hearts, including those thusfar considered characteristic for other tissues. 2. Significant differences were observed in the expression of the VEGF-A splice forms with respect to the myocardial layers and the location of the cardiac biopsy. 3. Repetitive and comparable results for samples with post-inflammatory etiology were obtained, and they revealed considerably higher amounts of VEGF-A isoforms compared to specimens with idiopathic etiology.
Automated On‐Line Preconcentration of Palladium on Different Sorbents and its Determination in Environmental Samples
Authors: Sánchez Rojas Fuensanta , Bosch Ojeda Catalina , Cano Pavón José Manuel 
Published: February 2007

AbstractThe determination of noble metals in environmental samples is of increasing importance. Palladium is often employed as a catalyst in chemical industry and is also used with platinum and rhodium in motor car catalytic converters which might cause environmental pollution problems. Two different sorbents for palladium preconcentration in different samples were investigated: silica gel functionalized with 1,5‐bis(di‐2‐pyridyl)methylene tbiocarbohydrazide (DPTH‐gel) and [1,5‐Bis(2‐pyridyl)‐3‐sulphophenyI methylene thiocarbonohydrazide (PSTH) immobilised on an anion‐exchange resin (Dowex 1×8‐200)]. The sorbents were tested in a micro‐column, placed in the auto‐sampler arm, at the flow rate 2.8 mL min‐1. Elution was performed with 4 M HCl and 4 M HNO3, respectively. Satisfactory results were obtained for two sorbents.
Increased α‐Adrenergic responsiveness in idiopathic raynaud's disease
Authors: Freedman Robert R. , Sabharwal Subhash C. , Desai Nagaraj , Wenig Paul , Mayes Maureen 
Published: February 2007

AbstractIn our study of 28 patients with idiopathic Raynaud's disease, the patients had significantly greater digital blood flow responses to intraarterial phenylephrine and clonidine than did normal control subjects. There were no group differences in finger blood flow responses to body heating, reflex cooling, digital ischemia, or to intraarterial tyramine or isoproterenol. There were also no group differences in blood pressure or heart rate during any procedure. These results suggest that patients with idiopathic Raynaud's disease have increased peripheral vascular α‐adrenergic receptor sensitivity and/or density compared with normal persons.
Insulin‐like growth factor stimulation of chondrocyte proteoglycan synthesis by human synovial fluid
Authors: Schalkwijk Joost , Joosten Leo A. B. , Van Den Berg Wim B. , Van Wyk Judson J. , Van Putte LEVINUSB. A. De 
Published: February 2007

AbstractWe investigated the role of insulin‐like growth factors (IGFs) as regulating factors of cartilage metabolism in human synovial fluid (SF), using a bovine explant culture system that was shown to respond to recombinant IGF‐1 in vitro. SF from rheumatoid arthritis (RA) patients and from control patients was found to stimulate chondrocyte proteoglycan synthesis in bovine articular cartilage. A monoclonal antibody directed primarily against IGF‐1 (and to some extent, IGF‐2) partially blocked the stimulatory action of serum and totally blocked the stimulation by SF. These findings indicate that IGFs are major regulating factors of cartilage proteoglycan synthesis in human SF. In addition, we measured serum and SF levels of IGF‐1 in RA patients and control patients, using a radioimmunoassay. No difference in immunoreactive serum IGF‐1 was detected between patients and controls. The IGF‐1 levels in SF were consistently lower than in serum, for both patient groups. No differences in IGF‐1 concentration were found between RA and non‐RA SF. The relevance of these data with respect to joint inflammation is discussed.
Characterization of two immunoglobulin VH genes that are homologous to human rheumatoid factors
Authors: Chen Pojen P. , Liu Ming‐Fei , Glass Charles A. , Sinha Suman , Kipps Thomas J. , Carson Dennis A. 
Published: February 2007

AbstractWe isolated a VH1 germline gene (Humhv 1263) that is closely related to the heavy chains of 2 human rheumatoid factors (RF), Bor and Kas. We also found that the 783 rearranged VH1 gene is actually 91–93% homologous to Bor and Kas, and it differs from a VH1 complementary DNA (51P1) by only a single base. Thus, 783 is likely to be the unmutated form of a germline VH gene that may encode the heavy chains of RF Bor and Kas and several other human monoclonal RF.
The effect of chemonucleolysis on serum keratan sulfate levels in humans
Authors: Block Joel A. , Schnitzer Thomas J. , Andersson GUNNARB. J. , Lenz Mary Ellen , Jeffery Rosemarie , McNeill Thomas W. , Thonar Eugene J‐M. A. 
Published: February 2007

AbstractSensitive measurements of serum keratan sulfate (KS), a glycosaminoglycan found in large quantities in the proteoglycans of human cartilage, can be obtained using an enzyme‐linked immunosorbent‐inhibition assay. Patients who are undergoing chemonucleolysis (CN) provide a clinical opportunity to monitor the large‐scale proteolytic degradation of cartilage. By measuring serum KS levels both pre‐ and post‐CN, we have demonstrated that serum KS levels rise predictably after CN, in a manner that reflects major catabolic events of cartilage.
Methyldopa‐induced systemic lupus erythematosus
Authors: Nordstrom David M. , West Sterling G. , Rubin Robert L. 
Published: February 2007

AbstractThirteen months after starting methyldopa therapy, a 55‐year‐old white male patient presented with a syndrome of hemolytic anemia, arthritis, photosensitivity, and a positive antinuclear antibody test result. Methyldopa‐induced antinuclear antibodies were mainly IgG, directed against class H1 histones. Antibodies to native DNA and nonhistone proteins were not detected. Upon withdrawal of methyldopa therapy, and with a short course of prednisone and danazol therapy, the patient's symptoms and hemolytic anemia resolved. His clinical symptoms and serologic abnormalities returned to normal and remained negative after 2 years of followup.
Mutant fibronectin gene in skin fibroblasts of sclerotic lesions from patients with progressive systemic sclerosis
Authors: Deguchi Yasuhiro , Negoro Shigeru , Kishimoto Susumu 
Published: February 2007

AbstractA mutant fibronectin gene was identified in skin fibroblasts obtained from sclerotic lesions of 7 patients with progressive systemic sclerosis. We found 2 point mutations adjacent to the cell‐attachment tetrapeptide DNA sequence in the cell‐binding domain of the fibronectin gene. This observation suggests that the mutant fibronectin is related to an integral component of sclerotic pathogenesis through abnormal cellular interactions.
Expression of the major rheumatoid factor cross‐reactive idiotype in juvenile rheumatoid arthritis
Authors: Ilowite Norman T. , Wedgwood Josiah F. , Bonagura Vincent R. 
Published: February 2007

AbstractThe major rheumatoid factor cross‐reactive idiotype (RF‐CRI), defined by prototypic monoclonal IgM rheumatoid factors, is expressed in high frequency by pokeweed mitogen–derived plasma cells from patients with rheumatoid arthritis who express RF in their sera. Unlike adults with rheumatoid arthritis, most patients with juvenile rheumatoid arthritis are seronegative for RF, as detected by classic IgG binding assays. We report that ∼50% of seronegative patients with juvenile rheumatoid arthritis express the RF‐CRI in high frequency among their pokeweed mitogen–derived plasma cells, and that ∼33% of patients express the RF‐CRI in high titer in their sera. The possible mechanisms for expression of an idiotypic marker of RF without expression of IgG binding activity by classic assays are discussed.
Requirements for flare reactions of joint inflammation induced in mice by cloned MT4+, Lyt‐2‐ T cells
Authors: Klasen Ina S. , Ladestein RenéE M. T. , van den Berg Wim B. , Benner Robbert 
Published: February 2007

AbstractJoint inflammation was induced in C57B1/6 mice by injection of cloned MT4+, Lyt‐2‐ T cells specific for the antigen methylated bovine serum albumin (mBSA), together with mBSA. In this model, after waning of the inflammation, flare reactions can be induced by a rechallenge with the specific antigen. Herein we show that such flare reactions can still be induced several weeks after waning of the joint inflammation, as was demonstrated both in normal C57B1/6 mice and in athymic C57B1 nude mice. The results in the latter group indicate that T cells of the recipient mice are not necessary for the elicitation of flare reactions. On histologic examination, the inflammatory infiltrates in the knee joints of the nude mice appeared to be mainly granulocytic. The cloned T cells persisted and remained functionally reactive in the knee joint for at least 2 weeks in the absence of the antigen, and thus, in the absence of inflammation. In view of the similarities between induced joint inflammation in mice and rheumatoid arthritis in humans, these data may be relevant to our understanding of the processes involved in the latter disease.
Factitious septic arthritis
Authors: Elliott T. G. , Burdge D. , Reid G. D. 
Published: February 2007

AbstractSeptic arthritis is an uncommon manifestation of factitious illness. We report 2 patients who developed septic arthritis of the knee after repeated self‐administered intraarticular injections. Multiple unusual infective agents were isolated. These cases illustrate malingering and Munchausen syndrome, 2 examples from the spectrum of factitious disease syndromes.
Very late activation antigen on synovial fluid T cells from patients with rheumatoid arthritis and other rheumatic diseases
Authors: Laffon A. , Sánchez‐madrid F. , Íz de Landázuri M. Ort , Énez Cuesta A. Jim , Ariza A. , Ossorio C. , Sabando P. 
Published: February 2007

AbstractWe used flow cytometry and immunoprecipitation techniques to study the expression of the activation molecules transferrin receptor, interleukin‐2 receptor, HLA–DR, and very late activation antigen 1 (VLA‐1) on purified T lymphocytes from peripheral blood and synovial fluid of 9 patients with rheumatoid arthritis and 7 patients with other rheumatic diseases. We found a T cell subset bearing VLA‐1 in synovial fluid from 8 rheumatoid arthritis patients and 4 patients with other rheumatic diseases. VLA‐1 was not found in peripheral blood T lymphocytes from either group.
Systemic vasculitis in association with human immunodeficiency virus infection
Authors: Calabrese Leonard H. , Estes Melinda , Yen‐Lieberman Belinda , Proffitt Max R. , Tubbs Raymond , Fishleder Andrew J. , Levin Kerry H. 
Published: February 2007

AbstractFourteen cases of vasculitis associated with human immunodeficiency virus infection have thus far been described. Five of these cases may be classified as angiocentric immunoproliferative disorders, including benign lymphocytic angiitis, lymphomatoid granuloma‐tosis, and angiocentric lymphoma. We report a case of benign lymphocytic angiitis of T cell lineage. Extensive studies found no evidence of viral antigens in the inflammatory infiltrates, and immunologic evaluation of the pathologic lesions revealed the infiltrating cells to be predominantly CD3+, CD8+, CD4−. A significant number of these lymphocytes demonstrated a deletion of T cell antigen receptor determinants. We believe that in certain cases of human immunodeficiency virus disease, there occurs a spectrum of lymphoproliferative disorders with angiocentric features that lead to the clinical picture of systemic necrotizing vasculitis. Clinicians should be aware of this association.
Immunosuppression with chlorambucil, versus placebo, for scleroderma. Results of a three‐year, parallel, randomized, double‐blind study
Authors: Furst Daniel E. , Clements Philip J. , Hillis Steven , Lachenbruch Peter A. , Miller Bruce L. , Sterz Mildred G. , Paulus Harold E. 
Published: February 2007

AbstractPlacebo and chlorambucil (0.05–0.1 mg/kg/day) were compared as treatments for scleroderma in a 3‐year, randomized, double‐blind, parallel study of 65 patients. Slopes of change over time, calculated for each organ system of each patient, failed to show significant differences between treatment groups. An immunosup‐pressive effect in the chlorambucil‐treated group was shown by decreases in the white blood cell counts (P = 0.02), platelet counts (P = 0.04), lymphocyte counts (P = 0.001), IgA/IgM concentrations (P ≤ 0.05), and wheal size on mumps skin tests (P = 0.02). Three years of immunosuppressive treatment with chlorambucil did not benefit this scleroderma population.
Cartilage proteoglycan depletion in acute and chronic antigen‐induced arthritis
Authors: Pettipher E. R. , Henderson B. , Hardingham T. , Ratcliffe A. 
Published: February 2007

AbstractWe examined the kinetics of proteoglycan (PG) depletion in rabbits with antigen‐induced arthritis. There was a rapid loss of PG from arthritic cartilage, reaching 35–40% at day 7. Thereafter, the rate of PG depletion declined, and by day 42, the maximum loss was 55–60%. The initial loss of PG was accompanied by the appearance of large amounts of sulfated glycosaminoglycans (GAGs) in the joint fluid (measured as total sulfated GAGs by dye binding and as keratan sulfate by radioimmunoassay). However, by day 14, the levels of sulfated GAGs in arthritic joint fluid declined to control levels, even though the cartilage demonstrated a sustained depletion of PG. The cartilage PG degradation observed in antigen‐induced arthritis could also be produced in normal animals by a single intraarticular injection of recombinant interleukin‐1. The acute loss of cartilage PG occurred independently of neutrophil accumulation, both in the case of antigen‐induced arthritis and after injection of interleukin‐1.
Changes in the metaphysis and diaphysis of the femur proximal to the knee in rabbits with experimentally induced inflammatory arthritis
Authors: Bogoch E. , Gschwend N. , Bogoch B. , Rahn B. , Perren S. 
Published: February 2007

AbstractWe quantified the cross‐sectional cortical bone area and remodeling rates in the ipsilateral femoral diaphysis and metaphysis of rabbits with carrageenan‐induced inflammatory monarthritis of the knee. Although osteogenesis rates were significantly elevated (approximately threefold in diaphyses and sixfold in metaphyses), the cross‐sectional bone area of the diaphysis was significantly diminished. Fivefold and sixfold increases in bone resorption rates were calculated in diaphyses and metaphyses, respectively. Other morphologic changes observed were porosity and net resorption of the anterior cortex and net accretion on the posteromedial cortex (“drift” of bone). These findings may have clinical significance with regard to the mechanisms and consequences of bone changes in patients with rheumatoid arthritis.