bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cognitive‐behavior therapy for Japanese patients with panic disorder: Acute phase and one‐year follow‐up results
Authors: Nakano Yumi , Lee Kiyoe , Noda Yumiko , Ogawa Sei , Kinoshita Yoshihiro , Funayama Tadashi , Watanabe Norio , Chen Junwen , Noguchi Yuka , Furukawa Toshiaki A. 
Published: June 2008

Aim:  The aim of this paper is to report the outcomes and follow‐up data of our cognitive behavioral therapy program for Japanese patients with panic disorder and to examine the baseline predictors of their outcomes.Methods:  Seventy outpatients with panic disorder with or without agoraphobia were treated with manualized group cognitive behavioral therapy.Results:  Fourteen patients (20%) did not complete the program. Among the completers, the average Panic Disorder Severity Scale score fell from 12.8 at baseline to 7.1 post‐therapy (44.7% reduction). This effectiveness was sustained for 1 year. While controlling for the baseline severity, the duration of illness and the baseline social dysfunction emerged as significant predictors of the outcome.Conclusions:  Our data suggest that group cognitive behavioral therapy for panic disorder can bring about as much symptom reduction among Japanese patients with panic disorder as among Western patients.
Adenocarcinoma arising from respiratory ciliated epithelium in benign cystic teratoma of the ovary: A case report with analyzes of the CT, MRI, and pathological findings
Authors: Yahata Tetsuro , Kawasaki Takashi , Serikawa Takehiro , Suzuki Mina , Tanaka Kenichi 
Published: June 2008

AbstractThe malignant transformation of mature cystic teratoma is rare, thus occurring in only 1–2% of all cases. The most common malignancy arising in mature cystic teratoma is squamous cell carcinoma. Adenocarcinoma occurs with less frequency. We herein present a patient with an ovarian mature cystic teratoma who demonstrated a malignant transformation to well‐differentiated adenocarcinoma. Malignant transformation was diagnosed preoperatively by contrast enhanced computed tomography (CT) and magnetic resonance imaging (MRI). Microscopically and immunohistochemically, the adenocarcinoma was considered to have arisen from the ciliated respiratory epithelium. After a 28‐month of follow‐up period, she remains free of the disease. This is the third reported case of adenocarcinoma arising in the respiratory epithelium of an ovarian mature cystic teratoma. Contrast enhanced CT and MRI are useful for making a preoperative diagnosis and an immunohistochemical study is helpful for defining its origin.
Establishing a reference value for the frequency of fetal movements using modified ‘count to 10’ method
Authors: Kuwata Tomoyuki , Matsubara Shigeki , Ohkusa Takashi , Ohkuchi Akihide , Izumi Akio , Watanabe Takashi , Suzuki Mitsuaki 
Published: June 2008

AbstractAims:  To establish a reference value for the frequency of fetal movements perceived by the mother during the second half of pregnancy.Methods:  The study subjects consisted of 705 low risk Japanese pregnant women who continuously received antenatal care. We asked women to record the time required to perceive 10 fetal movements (‘count to 10’ time) everyday. We asked women to record it, not at a fixed time (i.e. evening time), but whenever they felt the fetus move the most actively. The position during counting (i.e. sitting position) was also not specified, and thus we named this method as modified ‘count to 10’ method. Satisfactory recordings were obtained from 690 women, which we used for analysis.Results:  The ‘count to 10’ time was almost the same from 22 weeks (10.9; 7.3–18.0 (median; interquartile range)) until 32 weeks (10.0; 6.2–15.6), and it Thirty‐two weeks showed the shortest time, which gradually increased toward 40 weeks (14.8; 9.5–24.0). Its 90th percentile was approximately 25 and 35 min at 22–36 weeks and at 37–40 weeks, respectively.Conclusions:  For the first time we established a reference value for perceived fetal movements throughout the second half of pregnancy. The present modified ‘count to 10’ method requires less time than the previous method. Approximately 98% (690/705) of women gave us satisfactory recordings. This reference value may be of use in identifying mothers with decreased fetal movements.
ACE and MTHFR gene polymorphisms in unexplained recurrent pregnancy loss
Authors: Vettriselvi Venkatesan , Vijayalakshmi Krishnaswami , Paul Solomon F. D. , Venkatachalam Perumal 
Published: June 2008

AbstractAim:  To assess the association between polymorphisms in angiotensin converting enzyme and methylene tetrahydrofolate reductase genes and recurrent pregnancy loss by a case‐control study in South Indian women.Methods:  DNA was extracted from peripheral blood leukocytes of 104 women with Recurrent Pregnancy Loss (RPL) and 120 controls. Genotyping of ACE Insertion Deletion and MTHFR C677T polymorphism were carried out by PCR and PCR‐RFLP, respectively.Results:  No statistically significant difference was observed in the distribution of genotypes between cases and controls for ACE and MTHFR polymorphisms. Further, the combination of MTHFR and ACE genotypes failed to reveal an association.Conclusion:  In conclusion, the present study reveals lack of association of MTHFR C677T and ACE I/D polymorphisms in RPL in South Indian women. However, we cannot exclude the possibility that other polymorphisms of ACE and MTHFR genes could be associated with the disease and might be clinically useful as a marker to assess risk for RPL.
Frequency of network synchronization in the hippocampus marks learning
Authors: Ponomarenko Alexey A. , Li Jay‐Shake , Korotkova Tatiana M. , Huston Joseph P. , Haas Helmut L. 
Published: June 2008

AbstractThe synchronization of neuronal networks may be instrumental in plasticity and learning. Hippocampal high‐frequency oscillations (140–200 Hz, ‘ripples’) characteristic of consummatory behaviours are thought to promote memory formation. We recorded ripple oscillations from the CA1 area in temporal learning tasks. Rats learned to adjust their operant response to the timing of food reward delivery [fixed interval schedule (FI)]. The intrinsic frequency of ripples was elevated following the switch in reinforcement timing. Learning, as assessed from the response pattern, correlated with fluctuations of intraripple frequency and amplitude. Changes in motor activity did not account for the variability of ripple oscillations. At the same time, features of ripples were unaltered when the fixed interval of reward delivery was changed but did not depend on the lever press response. Thus, in addition to the known replay of neuronal firing patterns during ripple oscillations, the rhythm itself appears to be modulated in an experience‐specific way and represents a direct correlate of learning.
Cocaine decreases the expression of PSA‐NCAM protein and attenuates long‐term potentiation via glucocorticoid receptors in the rat dentate gyrus
Authors: Maćkowiak Marzena , Grzegorzewska Małgorzata , Budziszewska Bogusława , Chocyk Agnieszka , Hess Grzegorz , Wędzony Krzysztof 
Published: June 2008

AbstractThe present study investigated a potential role for glucocorticoid (GR) and mineralocorticoid (MR) receptors in the detrimental effects of single cocaine (COC) administration on both the number of polysialylated neural cell adhesion molecule (PSA‐NCAM)‐positive neurons and the induction of long‐term potentiation (LTP) in the rat dentate gyrus (DG). The effects of COC (15 mg/kg i.p.) on the number of PSA‐NCAM‐positive neurons and the induction of LTP observed 2 days after COC administration were abolished either by depleting circulating corticosterone after administration of metyrapone (100 mg/kg s.c. given 3 h before COC) or by pharmacologically blocking GRs using mifepristone (RU 38486, 10 mg/kg s.c. given 1 h before COC). Administration of the MR blocker spironolactone (50 mg/kg s.c. given 1 h before COC) did not alter the effects of COC on the number of PSA‐NCAM‐positive neurons or LTP induction. Results have also shown that COC does not change the rate of cell proliferation, as measured by the presence of Ki‐67 and the incorporation of bromodeoxyuridine (100 mg/kg i.p. given 2 h after COC) into the newly born cells in the DG 2 days after COC administration. Finally, we observed that GRs colocalized with some, but not all, PSA‐NCAM‐positive neurons, whereas MRs showed no colocalization with neurons positive for PSA‐NCAM in the DG. These data indicate that a single dose of COC may arrest hippocampal susceptibility to plastic changes and lead to functional impairments through the alteration of hippocampal structure and the formation of memory traces.
Regulation of cardiac sympathetic afferent reflex by GABAAand GABABreceptors in paraventricular nucleus in rats
Authors: Zhong Ming‐Kui , Shi Zhen , Zhou Li‐Min , Gao Juan , Liao Zhao‐hui , Wang Wei , Gao Xing‐Ya , Zhu Guo‐Qing 
Published: June 2008

AbstractThe aim of the present study was to determine the role of GABAAand GABABreceptors in paraventricular nucleus (PVN) in regulating cardiac sympathetic afferent reflex (CSAR). Under urethane (800 mg/kg) and α‐chloralose (40 mg/kg) anesthesia, renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) were recorded in sinoaortic‐denervated and cervical‐vagotomized rats. CSAR was evaluated based in the response of RSNA to epicardial application of capsaicin (0.3 nmol) or bradykinin (1 nmol). Bilateral PVN microinjection of the GABAAreceptor agonist isoguvacine (10 nmol) attenuated CSAR, while the GABABreceptor agonist baclofen (1 nmol) abolished CSAR. Both isoguvacine and baclofen greatly decreased baseline RSNA and MAP. The GABAAreceptor antagonist gabazine (0.1 nmol) had no significant effect on CSAR, but the GABABreceptor antagonist CGP‐35348 (10 nmol) enhanced CSAR. Gabazine caused greater increases in baseline RSNA and MAP than CGP‐35348. Vigabatrin (10 nmol), a selective GABA‐transaminase inhibitor which increases endogenous GABA level, abolished CSAR, and decreased baseline RSNA, MAP and HR. The effects of vigabatrin were antagonized by combined gabazine (0.1 nmol) and CGP‐35348 (10 nmol). The results indicate that activation of either GABAAor GABABreceptors in the PVN inhibits CSAR, while blockage of GABABreceptors in the PVN enhances CSAR. Endogenous GABA in the PVN could have an important role in regulating CSAR.
Aberrant trafficking of the high‐affinity choline transporter in AP‐3‐deficient mice
Authors: Misawa Hidemi , Fujigaya Hirofumi , Nishimura Takashi , Moriwaki Yasuhiro , Okuda Takashi , Kawashima Koichiro , Nakata Kazuko , Ruggiero Alicia M. , Blakely Randy D. , Nakatsu Fubito , Ohno Hiroshi 
Published: June 2008

AbstractThe high‐affinity choline transporter (CHT) is expressed in cholinergic neurons and efficiently transported to axon terminals where it controls the rate‐limiting step in acetylcholine synthesis. Recent studies have shown that the majority of CHT is unexpectedly localized on synaptic vesicles (SV) rather than the presynaptic plasma membrane, establishing vesicular CHT trafficking as a basis for activity‐dependent CHT regulation. Here, we analyse the intracellular distribution of CHT in the adaptor protein‐3 (AP‐3)‐deficient mouse model mocha. In the mocha mouse, granular structures in cell bodies are intensely labelled with CHT antibody, indicating possible deficits in CHT trafficking from the cell body to the axon terminal. Western blot analyses reveal that CHT on SV in mocha mice is decreased by 30% compared with wild‐type mice. However, no significant difference in synaptosomal choline uptake activity is detected, consistent with the existence of a large reservoir pool for CHT. To further characterize CHT trafficking, we established a PC12D‐CHT cell line. In this line, CHT is found associated with a subpopulation of synaptophysin‐positive synaptic‐like microvesicles (SLMV). The amounts of CHT detected on SLMV are greatly reduced by treating the cell with agents that halt AP‐dependent membrane trafficking. These results demonstrate that APs have important functions for CHT trafficking in neuronal cells.
Neurokinin receptor modulation of respiratory activity in the rabbit
Authors: Bongianni Fulvia , Mutolo Donatella , Cinelli Elenia , Pantaleo Tito 
Published: June 2008

AbstractThe respiratory role of neurokinin (NK) receptors was investigated in α‐chloralose‐urethane‐anaesthetized, vagotomized, paralysed and artificially ventilated rabbits by using bilateral microinjections (30–50 nL) of NK receptor agonists and antagonists. Microinjections were performed in a region located just caudal to the rostral expiratory neurons. This region displayed features similar to those of the pre‐Bötzinger complex (pre‐BötC) of adult cats and rats, and proved to produce excitatory respiratory effects in response to microinjections of d,l‐homocysteic acid. We used as agonists (0.1, 0.5 and 5 mm) substance P (SP), the NK1 receptor agonists [Sar9, Met(O2)11]‐SP and GR 73632, the NK2 receptor agonist NKA, the NK3 receptor agonist senktide, and as antagonists (5 mm) the NK1 receptor antagonist CP‐99,994 and the NK2 receptor antagonist MEN 10376. SP always increased respiratory frequency, but NK1 receptor agonists did not change respiratory variables. NKA and senktide at 5 mm increased respiratory frequency. CP‐99,994 caused increases in respiratory frequency and did not antagonize the effects of SP. MEN 10376 prevented the respiratory responses induced by NKA and reduced those provoked by SP. SP or the NK1 receptor agonists (5 mm) injected (1 μL) into the IV ventricle caused marked excitatory effects on respiration. The results suggest that NK2 and NK3, but not NK1, receptors are involved in the excitatory modulation of inspiratory activity within the investigated region and are consistent with the notion that the pre‐BötC neurons are important components of the inspiratory rhythm‐generating mechanisms.
Roles of the urologist and nurse from the perspective of patients with prostate cancer receiving luteinizing hormone‐releasing hormone analogue therapy
Authors: Lebret Thierry , Bouregba Alain 
Published: June 2008

OBJECTIVESTo establish the roles of the urologist, general practitioner (GP) and nurse from the perspective of the patient with prostate cancer receiving hormone therapy, and to assess patient satisfaction, in particular with management and information needs.PATIENTS AND METHODSPatients with prostate cancer receiving luteinizing hormone‐releasing hormone analogue (LHRH‐a) therapy were recruited by a representative sample of 58 French urologists (March to April 2007) and invited to complete a 42‐item questionnaire.RESULTSIn all, 350 representative patients participated in the study; >90% were totally or quite satisfied with the information given by their urologist at diagnosis and the start of treatment. Their main contact during treatment was with the nurse who gave the injection (84% of patients). The nurse’s main role was to provide clarity (60% of patients), guidance (35%) and support to the family (28%). Fewer patients discussed disease stage and progression with their nurse (29%) than with a doctor (urologist, 63%; or GP, 61%). Fewer also discussed treatment (24% vs 32%) but as many patients discussed the impact of their disease and treatment with their nurse as with their doctor (e.g. 33% discussed general health and fatigue with the nurse, vs 26% with the urologist). The need for contact with a health professional was greater during the early stages of treatment. Patients treated for <12 months with 3‐monthly injections were less likely to be in favour of spacing injections than patients treated for ≥3 years.CONCLUSIONThe patient consults the urologist for reliable information on disease and treatment, and to the GP for further support, if needed, but the nurse has the pivotal role. A 3‐monthly injection schedule enables regular face‐to‐face contact between the nurse and the patient and their family, and contributes towards the patient’s coping strategies and quality of life.
Highly specific detection of prostate‐specific antigen‐positive cells in the blood of patients with prostate cancer or benign prostatic hyperplasia, using a real‐time reverse‐transcription‐polymerase chain reaction method with improved sensitivity
Authors: Barbero Giovanna , Destefanis Paolo , Procida Simone , Mandili Giorgia , Ulliers Daniela , Ceruti Carlo , Fiori Cristian , Maule Milena M. , Fontana Dario , Giribaldi Giuliana , Turrini Franco 
Published: June 2008

OBJECTIVETo assess the presence of circulating prostate‐specific antigen (PSA)‐expressing cells in patients with prostate cancer or benign prostatic hyperplasia (BPH), and to determine their diagnostic usefulness using a highly sensitive quantitative real‐time reverse‐transcription polymerase chain reaction (qRT‐PCR) method.PATIENTS, SUBJECTS AND METHODSVenous blood samples were obtained from 175 patients with prostate cancer (12 metastatic and 163 not metastatic), 49 with BPH, and 50 healthy volunteers. To improve the specificity and sensitivity of the qRT‐PCR three innovative features were combined; a primer overlapping two adjacent exons to inhibit nonspecific amplification; a no‐end‐point first round amplification to increase the sensitivity; and a target‐specific primer for the RT phase to increase the specificity.RESULTSThe sensitivity of the method was 1 cell/mL of blood and the interassay coefficient of variation was 10.5%. None of the healthy subjects tested positively, while 9% of those with prostatic cancer and 14% with BPH had PSA‐positive cells in the blood. There was a positive association between a positive test and the National Comprehensive Cancer Network classification in the patients with newly diagnosed prostate cancer (P = 0.022). There were no additional statistically significant associations.CONCLUSIONOur results strongly indicate that although there were no false‐positive results and the sensitivity of the method was increased to maximal levels, a low frequency of positive results in patients with prostatic cancer and a high frequency of positive results in those with BPH seems to discourage the use of PSA‐positive circulating cells in the search for a clinical diagnosis of prostate cancer.
Efavirenz replacement by immediate full‐dose nevirapine is safe in HIV‐1‐infected patients in Cambodia
Authors: Laureillard D , Prak N , Fernandez M , Ngeth C , Moeung S , Riel V , Chhneang V , Song S , Quillet C , Piketty C 
Published: June 2008

BackgroundEfavirenz is used for the antiretroviral treatment of HIV/tuberculosis‐coinfected patients in developing countries. A switch to nevirapine is regularly carried out because of the cost and side effects of efavirenz. Pharmacokinetic studies suggested that nevirapine should be initiated at full dose when used as a substitute for efavirenz.ObjectivesThe aim of this study was to measure the cumulative incidence of adverse events (AEs) related to nevirapine in patients switched from efavirenz to immediate full‐dose nevirapine (FDN).MethodsIn 2001 an antiretroviral treatment programme was initiated with the first‐line regimen stavudine, lamivudine and efavirenz. In 2003, the fixed‐dose combination of stavudine, lamivudine and nevirapine was recommended. Thus, first‐line therapy was changed and FDN was initiated when patients were switched from efavirenz to nevirapine.ResultsBetween April and December 2004, 394 patients were switched from efavirenz to FDN. The cumulative incidence of AEs related to nevirapine was 13.2% [95% confidence interval (CI) 10.2–16.7] and that of severe AEs was 8.9% (95% CI 6.5–11.9). In women the incidence of AEs was 17.6% (95% CI 12.1–24.3) and that of severe AEs was 12.2% (95% CI 7.7–18.2).ConclusionsOur results indicate that an FDN switch from efavirenz does not appear to result in more AEs than when nevirapine is initiated with escalating doses. These data are particularly relevant in resource‐limited settings.
Hepatitis B or hepatitis C coinfection in HIV‐infected pregnant women in Europe
Authors: Landes M , Newell M‐L , Barlow P , Fiore S , Malyuta R , Martinelli P , Posokhova S , Savasi V , Semenenko I , Stelmah A , Tibaldi C , Thorne C 
Published: June 2008

ObjectivesThe aim of the study was to investigate the prevalence of and risk factors for hepatitis C or B virus (HCV or HBV) coinfection among HIV‐infected pregnant women, and to investigate their immunological and virological characteristics and antiretroviral therapy use.MethodsInformation on HBV surface antigen (HBsAg) positivity and HCV antibody (anti‐HCV) was collected retrospectively from the antenatal records of HIV‐infected women enrolled in the European Collaborative Study and linked to prospectively collected data.ResultsOf 1050 women, 4.9% [95% confidence interval (CI) 3.6–6.3] were HBsAg positive and 12.3% (95% CI 10.4–14.4) had anti‐HCV antibody. Women with an injecting drug use(r) (IDU) history had the highest HCV‐seropositivity prevalence (28%; 95% CI 22.8–35.7). Risk factors for HCV seropositivity included IDU history [adjusted odds ratio (AOR) 2.92; 95% CI 1.86–4.58], age (for ≥35 years vs. <25 years, AOR 3.45; 95% CI 1.66–7.20) and HBsAg carriage (AOR 5.80; 95% CI 2.78–12.1). HBsAg positivity was associated with African origin (AOR 2.74; 95% CI 1.20–6.26) and HCV seropositivity (AOR 6.44; 95% CI 3.08–13.5). Highly active antiretroviral therapy (HAART) use was less likely in HIV/HCV‐seropositive than in HIV‐monoinfected women (AOR 0.34; 95% CI 0.20–0.58). HCV seropositivity was associated with a higher adjusted HIV RNA level (+0.28log10 HIV‐1 RNA copies/mL vs. HIV‐monoinfected women; P=0.03). HIV/HCV‐seropositive women were twice as likely to have detectable HIV in the third trimester/delivery as HIV‐monoinfected women (AOR 1.95; P=0.049).ConclusionsAlthough HCV serostatus impacted on HAART use, the association between HCV seropositivity and uncontrolled HIV viraemia in late pregnancy was independent of HAART.
Assessing migraine disability by diary‐based measurement: relationship to the characteristics of individual headache attacks
Authors: Park J. W. , Shin H. E. , Kim J. S. , Lee K. S. 
Published: June 2008

Background and purpose:  Information on headache‐related disability helps clinicians create the optimal treatment plan for migraine sufferers. The estimation of disability must be determined by gathering the individual effects of multiple headache attacks within a given period of time. This study was aimed to examine migraine‐related disability from diary‐based assessment and to determine which elements of the headache characteristics are associated with disability.Methods:  Ninety‐two migraine patients were enrolled and contributed data on a total of 422 diary days of headache attacks. Each diary booklet was comprised of questions on headache features and disability comprising five items that originated from Migraine Disability Assessment (MIDAS).Results:  The average days recorded with disability for one or more of the three aspects of activities per headache attack was 0.55. Amongst the headache variables, pain intensity, nausea/vomiting, photophobia, type of abortive treatment and the employment status were significantly correlated with disability. On regression analysis, pain intensity, nausea/vomiting and employed work status were identified as significant predicting factors for migraine‐related disability.Conclusion:  These results demonstrated that a considerable amount of disability could occur during migraine attack and there are specified elements that act on the development of disability.