bluuebunny/crossref_metadata_2025_split huggingface dataset:
Albumin-loaded nanofiber for topical wound healing
Authors: Saadat Fatemeh , Azarbayjani Anahita Fathi 
Published: February 2024

Abstract
Background
Intravenous albumin administration increases blood circulation and enhances wound healing. Topical caffeine can induce vascularization and the formation of blood vessels on the skin. The purpose of this work is to explore for the first time the effect of topical albumin and caffeine on wound healing in rat models.
Methods
This work aimed to develop albumin and caffeine-loaded nanofibers by the electrospinning method and to evaluate their topical effect on wound healing. Nanofiber formation was assessed by SEM and considered using FT-IR spectroscopy.
Results
The therapeutic activity of topical albumin and caffeine was investigated on a full-thickness excision skin model. Results indicate faster healing of wounds covered with albumin. Albumin alone or in combination with caffeine effectively reduced the exposed wound area. Data show that daily albumin-loaded wound dressing displayed good healing properties and enhanced wound closure rate.
Conclusion
These findings may indicate the successful application of the sauce as a promising tool in wound healing therapy.
Escalating High-dimensional Imaging using Combinatorial Channel Multiplexing and Deep Learning
Authors: Keren Leeat , Ben-Uri Raz , Shabat Lior Ben , Bar-Tal Omer , Bussi Yuval , Maimon Noa , Haran Tal Keidar , Milo Idan , Elhanani Ofer , Rochwarger Alexander , Schürch Christian M. , Bagon Shai 
Published: February 2024

Abstract
Understanding tissue structure and function requires tools that quantify the expression of multiple proteins at single-cell resolution while preserving spatial information. Current imaging technologies use a separate channel for each individual protein, inherently limiting their throughput and scalability. Here, we present CombPlex (COMBinatorial multiPLEXing), a combinatorial staining platform coupled with an algorithmic framework to exponentially increase the number of proteins that can be measured from C up to 2C − 1, and is applicable to any mass spectrometry based or fluorescence-based microscopy platform. In CombPlex, every protein can be imaged in several channels, and every channel contains agglomerated images of several proteins. These combinatorically-compressed images are then decompressed to individual protein-images using deep learning and optimization. We perform feasibility experiments in silico and achieve accurate (F1=0.98, R=0.99) reconstruction for compressing the stains of twenty-two proteins to five imaging channels. We test our approach experimentally and obtain accurate (F1=0.97, R=0.93) images of seven proteins using three channels, both in fluorescence microscopy and in mass-based imaging. We demonstrate that combinatorial staining coupled with deep-learning decompression can serve to escalate the number of proteins measured using any imaging modality, without the need for specialized instrumentation. Coupling CombPlex with instruments for high dimensional imaging could pave the way to image hundreds of proteins at single-cell resolution in intact tissue sections.
Ocular infections by melanized fungi Curvularia lunata and Lasiodiplodia theobromae: antifungal susceptibility, clinical outcome, and clinico-microbiological correlation.
Authors: Mitra Sanchita , Garg Prashant , Murthy Somasheila , Jakati Saumya , Mohammed Ashik , Dave Vivek Pravin , Seba Esther , Kambar Swati 
Published: February 2024

Abstract
Purpose: Antifungal resistance is on the rise, with limited number of antifungals available for clinical use and very few new ones in the pipeline. Melanized fungal pathogens have been rarely studied for their antifungal susceptibility patterns and clinical outcomes, though they are the second most common cause of fungal ocular infections after hyaline fungi. Our study aims to report the antifungal susceptibility, clinical outcomes, and clinico-microbiological correlation of two common melanized fungi Curvularia lunata and Lasiodiplodia theobromae isolated from ocular infections.
Method: Antifungal susceptibility testing (AFST) was performed by broth microdilution technique as per standard guidelines in 30 isolates (17 C. lunata and 13 L. theobromae). Antifungal panel tested consisted of the polyenes amphotericin B and natamycin, the azoles voriconazole, ketoconazole, posaconazole, itraconazole and fluconazole, and the echinocandin caspofungin. Isolates resistant to more than or equal to two classes of antifungals were considered as multidrug resistant (MDR). DNA sequencing was performed for subset of isolates for species confirmation following conventional mycology. Statistical analysis consisted of both descriptive statistics and multivariate analysis.
Results: Isolates showed highest susceptibility to voriconazole (83.3% isolates), followed by natamycin (80%), fluconazole (80%), itraconazole (76.7%), ketoconazole (70%), posaconazole (66.7%), caspofungin (66.7%) and amphotericin B (63.3%), though MIC50 was on the lower side for all the antifungals. All patients were empirically administered topical natamycin with additional oral ketoconazole or intraocular voriconazole in select patients. Multivariate analysis suggested strong association between MDR and poor clinical outcome (p=0.03, odds ratio=7.8). All patients presented with microbial keratitis and one progressed to endophthalmitis. Surgical management with therapeutic penetrating keratoplasty (TPK) were required in 40% of patients. Good anatomical outcome was observed in 80% of patients, half of whom had good visual outcome too. Poor anatomical and visual outcome were observed in 20% of patients. DNA sequencing of subset of study isolates showed Curvularia lunata to be the highest Curvularia spp. study isolates (n=5/7). Histopathological examination of excised corneal buttons (TPK) showed fungal filaments in 66.7% (n=8/12) of cases.
Conclusion: Melanized fungi causing ocular infections have varying in-vitro susceptibility to different antifungal agents and clinical outcome. Voriconazole showed significantly higher and amphotericin B lower activity in-vitro for the fungi. The MDR isolates showed poorer clinical outcomes.
Visible light and dark-driven degradation of palm oil mill effluent (POME) over g-C3N4 and photorechargeable WO3
Authors: Razali Nur Aqilah Mohd , Salleh Wan Norharyati Wan , Mohamed Mohamad Azuwa , Aziz Farhana , Jye Lau Woei , Yusof Norhaniza , Ismail Ahmad Fauzi 
Published: February 2024

Abstract
The investigations of real industrial wastewater, such as palm oil mill effluent (POME), as a recalcitrant pollutant remain a subject of global water pollution concern. Thus, this work introduced the preparation and modification of g-C3N4 and WO3 at optimum calcination temperature, where they were used as potent visible light-driven photocatalysts in the degradation of POME under visible light irradiation. Herein, g-C3N4-derived melamine and WO3 photocatalyst were obtained at different calcination temperatures in order to tune their light absorption ability and optoelectronics properties. Both photocatalysts were proven to have their distinct phases, crystallinity levels, and elements with increasing temperature, as demonstrated by the ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction analysis (XRD), thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), Brunauer–Emmett–Teller (BET), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) results. Significantly, g-C3N4 (580°C) and WO3 (450°C) unitary photocatalysts exhibited the highest removal efficiency of POME without dilution due to good crystallinity, extended light absorption, high separation, and less recombination efficiency of electron-hole pairs. Furthermore, surprisingly, the superior energy storage photocatalytic performance with outstanding stability by WO3 achieved an approximately 10% increment during darkness, compared with g-C3N4 under visible light irradiation. Moreover, it has been proven that the WO3 and g-C3N4 photocatalysts are desirable photocatalysts for various pollutant degradations, with excellent visible-light utilization and favorable energy storage application.
Analysis and verification of ferroptosis-related genes in pediatric hepatoblastoma
Authors: Ye Xiaoshuo , Chen Wei , Xia Yongjie , Chen Zimin , Zheng Yuelan , Wang Bin 
Published: February 2024

Abstract

Background
Identifying effective biomarker in hepatoblastoma (HB) is important for predicting prognosis. This study committed to investigate the prognostic value of ferroptosis-related genes (FRGs) in HB.
Methods and Results
The two datasets of pediatric HB were obtained from Gene Expression Omnibus (GEO) database and analyzed differentially expressed genes (DEGs). Functional enrichment analysis was performed for these DEGs. Weighted gene co-expression network analysis (WGCNA) was used to screen the key modules. FRGs were obtained from the ferroptosis database. Subsequently, after identified of the candidate hub genes by the intersection of DEGs, key module genes and FRGs, least absolute shrinkage and selection operator (LASSO) and receiver operating characteristic (ROC) curves were finally applied to identify the hub genes. Two hub genes, TRL4 and TUBE1, were obtained with the AUC of 0.940 and 0.932. The gene set enrichment analysis (GSEA) was exerted to explore the signaling pathways related to the hub genes. The promoted expression of two hub genes in ferroptosis inducer, erastin-treated HB cell lines was verified via real-time qPCR. The effect of hub genes on viability and ferroptosis of HB cell line was verified in vitro. Specifically, the silence of TRL4 and TUBE1 could inhibit the ferroptosis and reverse the proliferation inhibition of HepG2 cells under erastin treating.
Conclusion
Ferroptosis-related genes TRL4 and TUBE1 emerge remarkable prognostic performance in pediatric HB as well as therapeutic target in the future. TRL4 and TUBE1 could function as tumor inhibiting factors in HB by promoting cell proliferation and prohibiting ferroptosis.
An Online Program of Seated Gentle Yoga &amp; Yogic Breathing Improves Symptoms in Scleroderma: A Quality Improvement Study
Authors: Balasubramanian Sundaravadivel , Blackwelder Amanda F , Wahlquist Amy E , Hendrix Katharine H. , Leyland Alexandria , Baskar Nivetha 
Published: February 2024

Abstract

Importance
Dry mouth is a common symptom experienced by scleroderma patients. Gentle yoga & yogic breathing improves salivation and other symptoms through parasympathetic dominance and may help scleroderma patients.
Objective
To conduct a pilot, feasibility study of an online Gentle Yoga and Yogic Breathing (GYYB) intervention for scleroderma patients during COVID-19 pandemic.
Design, Setting, and participants
A 60-minute GYYB exercise session was designed with consideration for the limitations of the scleroderma population and advertised in online communities. There were 99 participants registered over a seven week period. The sessions were led by certified Yoga Therapist weekly on Saturdays from 11 AM to 12 PM eastern time for seven weeks during COVID-19 pandemic (from 4/4/2020 to 5/16/2020) via the Zoom online teleconferencing tool. After each session participants had the opportunity to voluntarily complete feedback questionnaires about the session using Google Forms® designed for this purpose.
Results
Of 99 people that attended the sessions, 45 participants responded to the questionnaires. Over half (57.8%) respondents stated they would “Definitely” return to another session and participant satisfaction with the intervention was high (mean [SD] score 9.1 (1.3) on a scale of 1 [“Didn’t Like It at All”] to 10 [“Loved It”]). The majority reported liking both the yoga and breathing activities (75.6%) and 22.2% preferred the breathing activities. The majority (75.6%) found participation using computer or mobile phone “Very Easy”, with one participant reporting that it was “Very Difficult”. Most participants found doing yoga once daily (61.4%) for 30 minutes (56.6%) was most feasible. After one session, the majority (64.3%) reported they “Felt Better”, and no adverse effects were reported by any participant during the study.
Conclusion and Relevance
These data provide the first published evidence that an online yoga intervention may help address the need for low-cost, effective, non-pharmacological interventions to help people with scleroderma manage secondary symptoms and potentially improve their QoL.
Prevalence of post-COVID symptoms in a cohort of hospitalized patients in the North Coast of Colombia
Authors: Lozano Ana , Salcedo-Mejia Fernando , Dueñas Carmelo , Fernandez Juan Carlos , Arias Marly Jerez , Wilches Justo Paz , Zakzuk Nelson Rafael Alvis , Moyano-Tamara Lina , Alvis-Zakzuk Nelson J. , Zakzuk Josefina , Alvis-Guzman Nelson 
Published: February 2024

Abstract
Understanding the prevalence and severity of post-COVID-19 conditions among hospitalized patients is crucial for developing effective strategies for managing the long-term consequences of the disease. This study aimed to estimate the prevalence and severity of post-COVID-19 conditions in previously hospitalized COVID-19 patients. The study involved two phases: first, participants were contacted via phone call by trained personnel from the healthcare company and surveyed. In the second phase, two months after the telephone survey, a medical visit was conducted in the group of individuals who reported persistent symptoms in the previous call. Summary statistics such as mean, standard deviation (SD), interquartile range (IQR), 95%CI as well as absolute and relative frequencies of patients' baseline characteristics were reported. Changes were assessed through statistical tests for differences in means and proportions. Multivariate analyses were also conducted. The prevalence of at least one post-hospitalization condition after three months of COVID-19 hospitalization was 78.7 per 100 people. The most common symptoms included fatigue (55.4%), joint pain (46.0%), dyspnea (44.6%), sleep disorders (36.1%), anorexia (33.7%), and chest pain (31.7%). These conditions were frequent and disabling, experiencing at least one condition after three months. Within this group, more than 70% showed a deterioration in their health status (EQ5D-5L Scale) or experienced new or worsened disability in at least one domain of the Washington Group. Our study demonstrates that post-COVID-19 conditions in previously hospitalized patients are highly prevalent, which can adversely affect patients' quality of life and lead to increased demand for healthcare services.
Social threat avoidance depends on action-outcome predictability
Authors: Sequestro Matteo , Serfaty Jade , Grèzes Julie , Mennella Rocco 
Published: February 2024

Abstract

Adaptative action selection in threatening social contexts, for example when facing aggressive individuals, is core to social behavior. It is debated whether, when facing threat, initial action opportunities are mostly determined by automatic action tendencies – i.e., reactive stimulus-response (SR) associations – or by rapid and implicit goal-directed (GD) processes, which depend on the predicted consequences of available actions. To investigate the balance between SR and GD, we conducted three experiments manipulating the predictability of action-outcomes associations, in a novel approach-avoidance decision task in virtual reality. Participants presented a greater avoidance rate from angry individuals when the action-outcome association was predictable. Computationally, this was subtended by more efficient evidence accumulation, as indicated by an increased drift rate. Furthermore, cardiac deceleration around the time of choice was greater in the predictable condition and allowed the value attributed to the outcome to be better integrated into the decision. Finally, while most participants avoided angry individuals only under predictability, supporting a predominant role of GD processes, a minority of them avoided regardless of predictability. Overall, our results shed light on the computational architecture of social avoidance, opening interesting new research and clinical perspectives.
Beyond the Screen: Exploring the Dynamics of Social Media Influencers, Food Marketing, and Gendered Influences on Adolescent Diets
Authors: Amson Ashley , Bagnato Mariangela , Remedios Lauren , Pritchard Meghan , Soulene Sabir , Gillis Grace , Pauzé Elise , White Christine , Vanderlee Lana , Hammond David , Kent Monique Potvin 
Published: February 2024

Abstract

Background Adolescent obesity remains a public health concern, exacerbated by the risks posed by unhealthy food marketing, particularly on digital platforms. Social media influencers are increasingly utilized in digital marketing, yet their impact on adolescents remains understudied. This research explores the frequency of posts containing food products/brands, the most promoted food categories, the healthfulness of featured products, and the types of marketing techniques used by social media influencers popular with male and female adolescents. Methods A content analysis was conducted on posts between June 1, 2021, and May 31, 2022, posted by the top three social media influencers popular with males and female adolescents (13–17) on Instagram, TikTok, and YouTube (N = 1373). Descriptive statistics were applied to calculate frequencies for posts containing food products/brands, promoted food categories, product healthfulness, and marketing techniques. Health Canada’s Nutrient Profile Model classified product healthfulness. Results On average, social media influencers popular with males featured 1 food product/brand for every 2.5 posts compared to social media influencers popular with females who featured 1 food product/brand for every 6.1 posts. Water (27%) was the primary food category for social media influencers popular with females, while restaurants (24%) dominated for males. Social media influencers popular with males more commonly posted food products that were less healthy (89% versus 57%). Marketing techniques varied. Social media influencers popular with females used songs or music (53% vs 26%), the use of other influencers (26% vs 11%), appeals to fun or coolness (26% vs 13%), viral marketing (29% vs 19%), and appeals to beauty (11% vs 0%) more commonly. Comparatively, those popular with males more commonly used calls-to-action (27% vs 6%) and price promotions (8% vs 1%). Conclusion Social media influencers play a role in shaping adolescents' dietary preferences and behaviors. An often-overlooked aspect is the nuanced interplay of gender dynamics, where influencers contribute to the reinforcement or subversion of gendered expectations. As marketing plays a pivotal role in shaping dietary choices, understanding these gender-specific dynamics is essential for developing targeted interventions, policies, and educational initiatives aimed at promoting healthier food choices directed towards adolescents.
Thermal decomposition study of 4-methyloxybenzyl-glycoside by TG/DTA and on-line pyrolysis-photoionization mass spectrometry
Authors: Wu Lei , Wang Yiming , Yang Liutianyi , Jian Meiling , Ding Yu 
Published: February 2024

Abstract

A glycosidic bond flavor precursor of 4-methyloxybenzyl-2, 3, 4, 6-tetra-O-acetyl-β-D-glucopyranoside (MBGL) was synthesized by the modified Koenigs-Knorr reaction. The thermal decomposition behaviour and pyrolysis intermediate products of the glycoside were studied by simultaneous thermo-gravimetric/differential thermal analysis (TG/DTA) and synchrotron vacuum ultraviolet (VUV) photoionization mass spectrometry (PIMS). TG/DTA gave a TG-DTG curve, which showed the Tp of the glycoside was 246.7 ℃ with a largest mass loss rate. The total mass loss was 97.8%. The PIMS was used to identify the pyrolysis products of the MBGL at different temperatures. Some important pyrolysis intermediates, such as the ions of 4-methyloxybenzyl group at m/z 122 and glycon moiety at m/z 347, were detected by the PIMS. The results indicated that the MBGL was probably showed a different pyrolysis way compared with other glycoside. This work reported a good application of synchrotron VUV PIMS in thermal decomposition study of glycoside flavor precursor.
Synthesis, Characterization of Two New Schiff Base and Their PdII Complexes and Investigation of Palladium Catalyzed Cross-Coupling Reactions
Authors: Turan Nevin , Buldurun Kenan , Bingöl Mustafa , Çolak Naki 
Published: February 2024

Abstract
Two Schiff base ligands, S1 and S2, were synthesized from the reaction of 2-amino-5-ethyl-4-methylthiophene-3-carbonitrile with 2-hydroxybenzaldehyde and 4-nitrobenzaldehyde were investigated for their coordination to PdCl2(CH3CN)2. The prepared ligands S1 and S2 and the PdII complexes 1 and 2 were characterized by using FTIR, 1H, and 13C NMR, UV-Vis, TGA, elemental analysis, molar conductivity, mass, and magnetic susceptibility. The characterization data agree well with the formulation of ligands S1 and S2 and complexes 1 and 2. The geometries of the metal chelate were discussed with the help of magnetic and spectroscopic measurements. Finally, the catalytic potential of the synthesized PdII complexes for Suzuki-Miyaura and Mizoroki-Heck coupling reactions was investigated using GC-MS. As a result, it was observed that the palladium complexes are the active catalysts in suitable Suzuki-Miyaura and Mizoroki-Heck C-C coupling reactions.
Ultrasound stimulated microbubbles cavitation enhanced the therapeutic effect of colchicine in rats with acute gouty arthritis
Authors: Zhang Jicheng , Bo Gou , Wang Tian-rui , Li Yan-hui , Dang Wan-tai , Wen Wen , Liu Jian 
Published: February 2024

Abstract
Objective
To investigate whether cavitation induced by ultrasound stimulated microbubbles (USMB) can enhance the therapeutic effect of colchicine and reduce its side effects on acute gouty arthritis (AGA) in rats.
Materials and Methods
Each rat underwent right ankle joint contrast-enhanced ultrasound (CEUS) before and following cavitation treatment. The area under the curve (AUC) of CEUS before and after cavitation and among groups were compared to contrast the impact on blood perfusion of the ankle joint in rats with cavitation. The symptoms of AGA were evaluated based on 24-hour ankle joint swelling degree and gait score. The infiltration of neutrophils was assessed using the Hematoxylin-eosin(HE)staining, while the inflammation and dilation of blood vessels were measured by and plasma interleukin-1β (IL-1β) and endothelial nitric oxide synthase (eNOS). Moreover, the magnitude of side effects was analyzed by gastrointestinal reaction score.
Results
The AUC after cavitation induced by USMB were significantly higher than those before cavitation. When compared with groups without cavitation, the AUC were markedly higher in groups treated with cavitation (all p<0.01). In groups treated with cavitation combined with colchicine, the degree of ankle swelling, gait scores, and the level of plasma IL-1β in AGA rats were lower, while the concentration of plasma eNOS was higher, group treated with colchicine alone (all p<0.05). HE staining showed that cavitation combined with colchicine helped to reduce the neutrophil synovial infiltration of ankle joint and decrease the gastrointestinal reaction score in rats with AGA. Compared with the three groups receiving drug treatment, the incidence of gastrointestinal reaction score in the low concentration group was significantly lower than that in the high concentration groups (all p<0.05).
Conclusion
The cavitation induced by USMB could significantly enhance the blood perfusion of ankle joint in rats with AGA, leading to the increase in therapeutic effect of colchicine and reduce the dose used of colchicine, thus minimizing the side effects associated with it.