bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of gene dosage on cognitive ability: A function-based association study across brain and non-brain processes
Authors: Huguet Guillaume , Renne Thomas , Poulain Cécile , Dubuc Alma , Kumar Kuldeep , Kazem Sayeh , Engchuan Worrawat , Shanta Omar , Douard Elise , Proulx Catherine , Jean-Louis Martineau , Saci Zohra , Mollon Josephine , Schultz Laura M , Knowles Emma E M , Cox Simon R. , Porteous David , Davies Gail , Redmond Paul , Harris Sarah E. , Schumann Gunter , Dumas Guillaume , Labbe Aurélie , Pausova Zdenka , Paus Tomas , Scherer Stephen W , Sebat Jonathan , Almasy Laura , Glahn David C , Jacquemont Sébastien 
Published: April 2024

AbstractGenomic Copy Number Variants (CNVs) that increase risk for neurodevelopmental disorders are also associated with lower cognitive ability in general population cohorts. Studies have focussed on a small set of recurrent CNVs, but burden analyses suggested that the vast majority of CNVs affecting cognitive ability are too rare to reach variant-level association. As a result, the full range of gene-dosage-sensitive biological processes linked to cognitive ability remains unknown.To investigate this issue, we identified all CNVs >50 kilobases in 258k individuals from 6 general population cohorts with assessments of general cognitive abilities. We performed a CNV-GWAS and functional burden analyses, which tested 6502 gene-sets defined by tissue and cell-type transcriptomics as well as gene ontology disrupted by all rare coding CNVs.CNV-GWAS identified a novel duplication at 2q12.3 associated with higher performance in cognitive ability. Among the 864 gene-sets associated with cognitive ability, only 11% showed significant effects for both deletions and duplication. Accordingly, we systematically observed negative correlations between deletion and duplication effect sizes across all levels of biological observations. We quantified the preferential effects of deletions versus duplication using tagDS, a new normalized metric. Cognitive ability was preferentially affected by cortical, presynaptic, and negative-regulation gene-sets when duplicated. In contrast, preferential effects of deletions were observed for subcortical, post-synaptic, and positive-regulation gene-sets. A large proportion of gene-sets assigned to non-brain organs were associated with cognitive ability due to low tissue specificity genes, which were associated with higher sensitive to haploinsufficiency. Overall, most biological functions associated with cognitive ability are divided into those sensitive to either deletion or duplications.
Cytoadhesion ofPlasmodium falciparum-infected red blood cells changes the expression of cytokine-, histone- and antiviral protein-encoding genes in brain endothelial cells
Authors: Allweier Johannes , Bartels Michael , Torabi Hanifeh , Martinez Tauler Maria del Pilar , Metwally Nahla Galal , Roeder Thomas , Gutsmann Thomas , Bruchhaus Iris 
Published: April 2024

ABSTRACTMalaria remains a significant global health problem, mainly due toPlasmodium falciparum, which is responsible for most fatal infections. Infected red blood cells (iRBCs) evade spleen clearance by adhering to endothelial cells (ECs), triggering capillary blockage, inflammation, endothelial dysfunction and altered vascular permeability, prompting an endothelial transcriptional response. The iRBCIT4var04/HBEC-5i model, where iRBCs present IT4var04 (VAR2CSA) on their surface, was used to analyse the effects of iRBC binding on ECs at different temperature (37°C vs. 40°C). Binding of non-infected RBCs (niRBCs) and fever alone altered the expression of hundreds of genes in ECs. Comparing the expression profile of HBEC-5i cells cultured either in the presence of iRBCs or in the presence of niRBCs revealed significant upregulation of genes linked to immune response, nucleosome assembly, NF-kappa B signaling, angiogenesis, and antiviral immune response/interferon-alpha/beta signaling. Raising the temperature to 40°C, simulating fever, led to further upregulation of many genes, particularly those involved in cytokine production and angiogenesis. In summary, the presence of iRBCs stimulates ECs, activating several immunological pathways and affecting antiviral (- parasitic) mechanisms and angiogenesis. Our data uncovered the induction of the interferon-alpha/beta signaling pathway in ECs in response to iRBCs.
Secretion and Periplasmic Activation of a Potent Endonuclease in E. coli
Authors: Soltani Mehran , Swartz James R. 
Published: April 2024

AbstractSm Endonuclease (SmEn) is a promiscuous, highly active nuclease widely used in protein purification, 2D protein gels, and gene and cell therapy. We aimed to recombinantly and economically produce this reagent using E. coli. Despite widespread application of E. coli for recombinant production of proteins, cytoplasmic expression of this protein resulted in no activity accumulation. We therefore investigated translocation of SmEn to the periplasm of E. coli by evaluating several signal sequences, E. coli host cells, and incubation conditions. For rapid feedback, we developed a crude lysate-based nuclease activity assay that enabled convenient screening and identified suitable conditions for active SmEn accumulation. Signal sequence selection was most influential with additional benefit gained by slowing synthesis either using the transcriptionally weakened strain, C43 (DE3) or by reducing incubation temperature. While our study provides valuable insights for optimizing a nuclease translocation and reducing production costs, more research is needed to explore the influence of mRNA secondary structure at the translation initiation region on protein expression and translocation. Overall, our rapid screening assay facilitated the development of an effective production process for a protein with potential cytoplasmic toxicity as well as the need of disulfide bond formation.
Simple recombinant monoclonal antibody production fromE. coli
Authors: Baker Karen , Eastwood Tara A. , Garcia Esther , Lennon Christopher , Mulvihill Daniel P. 
Published: April 2024

AbstractAntibodies are valuable biological reagents used in a wide range of discovery research, biotechnology, diagnostic and therapeutic applications. Currently both commercial and lab scale antibody production is reliant on expression from mammalian cells, which can be time consuming and requires use of specialist facilities and costly growth reagents. Here we describe a simple, rapid and cheap method for producing and isolating functional monoclonal antibodies and antibody fragments from bacterial cells that can be used in a range of laboratory applications. This simple method only requires access to basic microbial cell culture and molecular biology equipment, making scalable in-house antibody production accessible to the global diagnostics, therapeutics and molecular bioscience research communities.
Doubly precarious immigrant academics: professional identities and work integration of a highly skilled precariat in Canadian higher education
Authors: Hari Amrita , Nardon Luciara , Palic Dunja 
Published: April 2024

Purpose Educational institutions are investing heavily in the internationalization of their campuses to attract global talent. Yet, highly skilled immigrants face persistent labor market challenges. We investigate how immigrant academics experience and mitigate their double precarity (migrant and academic) as they seek employment in higher education in Canada.Design/methodology/approach We take a phenomenological approach and draw on reflective interviews with nine immigrant academics, encouraging participants to elaborate on symbols and metaphors to describe their experiences.Findings We found that immigrant academics constitute a unique highly skilled precariat: a group of professionals with strong professional identities and attachments who face the dilemma of securing highly precarious employment (temporary, part-time and insecure) in a new academic environment or forgoing their professional attachment to seek stable employment in an alternate occupational sector. Long-term, stable and commensurate employment in Canadian higher education is out of reach due to credentialism. Those who stay the course risk deepening their precarity through multiple temporary engagements. Purposeful deskilling toward more stable employment that is disconnected from their previous educational and career accomplishments is a costly alternative in a situation of limited information and high uncertainty.Originality/value We bring into the conversation discussions of migrant precarity and academic precarity and draw on immigrant academics’ unique experiences and strategies to understand how this double precarization shapes their professional identities, mobility and work integration in Canadian higher education.
Visitor’s emotional experience in predicting destination image, satisfaction and intention to revisit: a spa tourism perspective
Authors: Suban Syed Ahamed 
Published: April 2024

PurposeThis study intend to investigate a theoretical model looking at how particular tourist emotions, such as “joy,” “love,” and “positive surprise,” might predict their behavior by looking at how satisfied they are with their whole experience when visiting spas, and to examine the relationship of emotional experience, destination image, satisfaction and intention to revisit for spa tourism.Design/methodology/approachA sample of 345 individuals who traveled to Alleppey as domestic tourists participated in the research study. A non-probability (purposive) sampling method in this study. The structural model was analyzed using Structural Equation modeling (SEM), and the path coefficients were examined to test the hypotheses.FindingsThe results supported the hypotheses, indicating that specific emotions, image of the destination, and satisfaction significantly impacted tourists' intentions to revisit Alleppey as a spa tourism destination. This study demonstrated that “emotions of joy, love, and positive surprise” have a considerable influence on the image of the destination and satisfaction. The findings reveal a substantial correlation between satisfaction and behavioral intention (“Intention to revisit”). The research suggests that a higher degree of satisfaction would encourage visitors to revisit the location.Research limitations/implicationsThe research suggests that a higher degree of satisfaction would encourage visitors to revisit the location. This research offers vital information for developing, planning, and putting into practice tourism policies in the spa tourism sector. This article focuses on domestic travelers who travel to Alleppey, so the conclusions may not be relevant to research utilizing foreign tourists.Originality/valueAccording to the literature study, and to the authors` knowledge, only limited number of studies that look at spa tourism from a wellness perspective. Additionally, Alleppey is used in the study as the study’s setting, providing insight into the visitor experiences of this expanding spa tourism business. This study gives understanding about how emotional experience predicts behavioral intentions.
Study on a smart knee sleeve based on piezoresistive strain sensing for stride estimation
Authors: Raji Rafiu King , Wei Yini , Diao Guiqiang , Tang Zilun 
Published: April 2024

PurposeDevices for step estimation are body-worn devices used to compute steps taken and/or distance covered by the user. Even though textiles or clothing are foremost to come to mind in terms of articles meant to be worn, their prominence among devices and systems meant for cadence is overshadowed by electronic products such as accelerometers, wristbands and smart phones. Athletes and sports enthusiasts using knee sleeves should be able to track their performances and monitor workout progress without the need to carry other devices with no direct sport utility, such as wristbands and wearable accelerometers. The purpose of this study thus is to contribute to the broad area of wearable devices for cadence application by developing a cheap but effective and efficient stride measurement system based on a knee sleeve.Design/methodology/approachA textile strain sensor is designed by weft knitting silver-plated nylon yarn together with nylon DTY and covered elastic yarn using a 1 × 1 rib structure. The area occupied by the silver-plated yarn within the structure served as the strain sensor. It worked such that, upon being subjected to stress, the electrical resistance of the sensor increases and in turn, is restored when the stress is removed. The strip with the sensor is knitted separately and subsequently sewn to the knee sleeve. The knee sleeve is then connected to a custom-made signal acquisition and processing system. A volunteer was employed for a wearer trial.FindingsExperimental results establish that the number of strides taken by the wearer can easily be correlated to the knee flexion and extension cycles of the wearer. The number of peaks computed by the signal acquisition and processing system is therefore counted to represent stride per minute. Therefore, the sensor is able to effectively count the number of strides taken by the user per minute. The coefficient of variation of over-ground test results yielded 0.03%, and stair climbing also obtained 0.14%, an indication of very high sensor repeatability.Research limitations/implicationsThe study was conducted using limited number of volunteers for the wearer trials.Practical implicationsBy embedding textile piezoresistive sensors in some specific garments and or accessories, physical activity such as gait and its related data can be effectively measured.Originality/valueTo the best of our knowledge, this is the first application of piezoresistive sensing in the knee sleeve for stride estimation. Also, this study establishes that it is possible to attach (sew) already-knit textile strain sensors to apparel to effectuate smart functionality.
Determinants of investment-related FinTech services among retail investors of India: a multi-group analysis using PLS-SEM
Authors: Jha Shweta , Dangwal Ramesh Chandra 
Published: April 2024

Purpose
The purpose of this study is to investigate the factors affecting behaviour intention (BI) to use and actual usages of investment-related FinTech services among the zoomers (Gen Z) and millennials (Gen M) retail investors of India.

Design/methodology/approach
The study explores the predictive relevance of actual adoption behaviour among the two different age categories of Indian retail investors. It uses the Unified Theory of Acceptance and Use of Technology-2 and the prospect theory framework as guiding frameworks. Data has been collected from 294 retail investors, actively engaged in the investment-related FinTech services. The multi-group analysis using variance-based partial least square structured equation modelling has been used to compare the two groups. The invariance between the two groups was achieved through measurement invariance assessment.

Findings
The study reveals distinct factors significantly affecting BI to use investment-related FinTech services among Gen Z and Gen M retail investors are performance expectancy (PE) to BI, perceived risk (PR) to BI, price value (PV) to BI and PR to service trust (ST).

Research limitations/implications
This study provides insights for financial providers and policymakers, emphasizing different factors influencing BI to use investment-related FinTech services in both age groups. Notably, habit emerges as a common factor influencing the actual usage of investment-related FinTech services across Gen M and Gen Z retail investors in India.

Originality/value
This study explores the heterogeneous behaviour of the heterogenous population in the domain of technological adoption of investment-related FinTech services in India.
Digital technologies and the evolution of the management accounting profession: a grounded theory literature review
Authors: Arkhipova Daria , Montemari Marco , Mio Chiara , Marasca Stefano 
Published: April 2024

Purpose
This paper aims to critically examine the accounting and information systems literature to understand the changes that are occurring in the management accounting profession. The changes the authors are interested in are linked to technology-driven innovations in managerial decision-making and in organizational structures. In addition, the paper highlights research gaps and opportunities for future research.

Design/methodology/approach
The authors adopted a grounded theory literature review method (Wolfswinkel et al., 2013) to achieve the study’s aims.

Findings
The authors identified four research themes that describe the changes in the management accounting profession due to technology-driven innovations: structured vs unstructured data, human vs algorithm-driven decision-making, delineated vs blurred functional boundaries and hierarchical vs platform-based organizations. The authors also identified tensions mentioned in the literature for each research theme.

Originality/value
Previous studies display a rather narrow focus on the role of digital technologies in accounting work and new competences that management accountants require in the digital era. By contrast, the authors focus on the broader technology-driven shifts in organizational processes and structures, which vastly change how accounting information is collected, processed and analyzed internally to support managerial decision-making. Hence, the paper focuses on how management accountants can adapt and evolve as their organizations transition toward a digital environment.
Stress response and phytoextraction potential of two Noccaea caerulescens populations in multicontaminated soil
Authors: Sherri Mohammad Chafik , Sirguey Catherine , Kanso Ali , Hamze Kassem , Ouvrard Stéphanie 
Published: April 2024

AbstractMulticontamination of soils by various organic and inorganic pollutants is considered as an obstacle for the development of hyperaccumulator plants and phytoextraction of metals. The aim of this study was to investigate the effect of polycyclic aromatic hydrocarbons (PAHs) in combination with trace elements on the antioxidant response and phytoextraction efficiency of two populations of the hyperaccumulator Noccaea caerulescens from either a metalliferous (Ganges) or a nonmetalliferous (Chavignée) site. Plants were grown for 17 days in soil containing moderate concentrations of trace elements and under the effect of phenanthrene (PHE), a PAH stress model. In general, exposure to PHE resulted in a reduction of growth parameters, together with the upregulation of antioxidant enzymes and compounds and limitations in nutrient uptake and heavy metal extraction in N. caerulescens. Variations were observed in the extent of enzymatic activities and the amount of metals extracted between the two populations studied. Plants from Chavignée exhibited a slightly more tolerant response to PHE stress than those from Ganges. The presence of PHE in the soil proved highly toxic to N. caerulescens, resulting in low numbers of survivors. Nevertheless, the differences observed between the two populations with regard to growth, metal extraction, and antioxidant defense responses suggest that the difference in defense capacity may ensure different tolerance. This difference may be linked to adaptations acquired by each population according to the soil type from which it originates. However, these results need to be confirmed by further experiments.
Institutional quality and environmental sustainability nexus: Fresh evidence of most polluted countries in a CS–ARDL framework
Authors: Alharthi Majed , Hassan Muhammad Shahid , Huang Jianjie , Mahmood Haider , Arshad Hassan 
Published: April 2024

AbstractInstitutional quality (IQ) would contribute to environmental sustainability and could be helping to verify the Environmental Kuznets curve in any economy. To test whether IQ is sufficient enough to control pollution in the most polluted economies, the present research investigates the effects of IQ on CO2 emissions controlling economic growth, globalization, and energy proxies in the models of the 33 most polluted countries from 1990 to 2021 by using cross‐sectional dependence techniques. The long‐run results corroborate the Environmental Kuznets curve in the sample countries. Moreover, clean energy reduces and fossil fuels increase CO2 emissions. Furthermore, globalization and regulatory quality reduce CO2 emissions. The short‐run findings also validate the long‐run results. In addition, we also find the bidirectional causality of CO2 emissions with economic growth, clean energy, fossil fuels, and globalization. Moreover, one‐way causality is found from CO2 emissions to regulatory quality and political stability. We recommend promoting trade globalization, clean energy usage, and regulatory quality to support environmental sustainability.
The OPI•AID Zone Tool as a composite outcome for postoperative pain management quality—A protocol for an observational pilot study
Authors: Karlsen Anders Peder Højer , Tran Trang Xuan Minh , Mathiesen Ole , Jakobsen Janus Christian , Meyhoff Christian S. , Lunn Troels Haxholdt , Olsen Markus Harboe 
Published: April 2024

AbstractBackgroundManaging postoperative pain while minimizing opioid‐related adverse drug events (ORADEs) remains a significant challenge. The OPI•AID Zone Tool is proposed as a novel clinical decision support tool that ‐ both graphically and in a scoring‐system ‐ represents the relationship between pain management and the occurrence of ORADEs, aiming to enhance patient outcomes in postoperative care. The OPI•AID Zone Tool places pain score on the x‐axis and an ORADE score on the y‐axis, and stratifies patients into five zones to reflect the composite impact of pain severity and ORADEs on the quality of postoperative patient care. The study will have two key aims: (1) to explore whether the OPI•AID Zone Tool can function as a composite outcome measure for postoperative pain and ORADEs, and (2) to evaluate the use of the OPI•AID Zone Tool in visual presentations and for evaluation of patients’ postoperative pain management quality.MethodsThis prospective observational cohort study will include 200 adults undergoing various surgical procedures in general anesthesia with a subsequent stay in the post‐anesthesia care unit (PACU) at Bispebjerg Hospital, Denmark.Substudy 1 primary outcome: To assess whether a zone score in the OPI•AID Zone Tool is associated with patient‐perceived health (EQ VAS), quality of recovery (QoR‐PACU), and time to discharge readiness in PACU, and if the zone score has a stronger association than pain and ORADE score in themselves.Substudy 2 primary outcome: To assess how the use of intraoperative non‐opioid analgesics impact where patients are placed in the OPI•AID Zone Tool's XY scatterplot right after surgery.To assess if patients who receive more comprehensive non‐opioid analgesic basic regimens, generally fall into lower zones.ConclusionThe OPI•AID Zone Tool could potentially be a valuable clinical decision‐making tool for optimizing postoperative care by simultaneously addressing pain management and the risk of ORADEs. By computing a composite measure of these two critical outcomes, the tool could guide more nuanced and patient‐centered analgesic regimens, potentially improving patient satisfaction and operational efficiency in postoperative settings. The tool's applicability will be explored in this observational pilot and followed up in a planned series of studies (opiaid.dk).