bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of gas chromatography–mass spectroscopy method for determination of prilocaine HCl in human plasma using internal standard methodology
Authors: Kadioglu Yucel , Atila Alptug 
Published: June 2007

AbstractThe accurate determination of prilocaine HCl levels in plasma is important in both clinical and pharmacological/toxicological studies. Prilocaine HCl is quickly hydrolyzed to o‐toluidine, causing methemoglobinemia. For this, the present work describes the methodology and validation of a GC‐MS assay for determination of prilocaine HCl with lidocaine HCl as internal standard in plasma. The validation parameters of linearity, precision, accuracy, recovery, specificity, limit of detection and limit of quantification were studied. The range of quantification for the GC‐MS was 20–250 ng/mL in plasma. Within‐day and between‐day precision, expressed as the relative standard deviation (RSD) were less than 6.0%, and accuracy (relative error) was better than 9.0% (n = 6). The analytical recovery of prilocaine HCl and IS from plasma has averaged 94.79 and 96.8%, respectively. LOQ and LOD values for plasma were found to be 20 and 10 ng/mL, respectively. The GC‐MS method can be used for determination from plasma of prilocaine HCl in routine measurement as well as in pharmacokinetic studies for clinical use. Copyright © 2007 John Wiley & Sons, Ltd.
Separation and determination of psoralen and isopsoralen by microemulsion electrokinetic chromatography
Authors: Zhang Huige , Zhu Jinhua , Qi Shengda , Chen Xingguo , Hu Zhide 
Published: June 2007

AbstractThe use of microemulsion electrokinetic chromatography was proposed to separate psoralen and isopsoralen in Psoralea corylitolia L. and its preparations. After conducting a series of optimizations, baseline separation was obtained for the analytes under the optimum conditions [sodium dodecyl sulfate 1.05% (m/v), ethyl acetate 0.96% (v/v), butan‐1‐ol 0.24% (v/v), 25 mm borate, pH 8.5, applied voltage 17.5 kV and detection at 254 nm]. Regression equations revealed linear relationships (correlation coefficients 0.9997 for psoralen and 0.9999 for isopsoralen) between the peak area of each analyte and the concentration. The limits of detection (defined as a signal‐to‐noise ratio of about 3) were 0.42 µg/mL for psoralen and 0.32 µg/mL for isopsoralen, respectively. The analytes were successfully determined with recoveries ranging from 95.50 to 102.03%. The method has been successfully applied for the analysis of psoralen and isopsoralen in medical samples. Furthermore, a simple and effective extraction method, with methanol in an ultrasonic water bath for 20 min three times, was used for sample preparation. Copyright © 2007 John Wiley & Sons, Ltd.
Identification of estrogenic/anti‐estrogenic compounds in diesel exhaust particulate extract
Authors: Noguchi Keiko , Toriba Akira , Chung Sang Woon , Kizu Ryoichi , Hayakawa Kazuichi 
Published: June 2007

AbstractDiesel exhaust particulate extract (DEPE) was obtained from diesel exhaust particulates with Soxhlet extraction using dichloromethane. After separating DEPE into 11 fractions by liquid–liquid extraction, the neutral fraction (N) showed anti‐estrogenic activity and the weak acid (phenol) fraction (WAP) showed estrogenic and anti‐estrogenic activities by a yeast two‐hybrid assay system expressing human estrogen receptor α. Both fractions were thoroughly fractionated by silica gel column chromatography and reversed‐phase HPLC. In the WAP fraction, 3‐methyl‐4‐nitrophenol and 2,6‐dimethyl‐4‐nitrophenol were identified by LC‐MS/MS as estrogenic compounds. This is the first study to identify 2,6‐dimethyl‐4‐nitrophenol in DEPE and the first study to show that it is an estrogenic compound. In the N fraction, 1‐hydroxypyrene was also identified by LC‐MS/MS as an anti‐estrogenic compound. Copyright © 2007 John Wiley & Sons, Ltd.
Quality evaluation of Radix Stemonae through simultaneous quantification of bioactive alkaloids by high‐performance liquid chromatography coupled with diode array and evaporative light scattering detectors
Authors: Li Song‐Lin , Jiang Ren‐Wang , Hon Po‐Ming , Cheng Ling , Xu Hong‐Xi , Greger Harald , But Paul Pui‐Hay , Shaw Pang‐Chui 
Published: June 2007

AbstractA high‐performance liquid chromatography coupled with diode array detection and evaporative light scattering detection (HPLC‐DAD‐ELSD) method was developed to simultaneously quantify six major bioactive alkaloids belonging to different structure types in Radix Stemonae, Bai‐Bu in Chinese, a traditionally used antitussive and insecticidal medicinal material in China and other countries of Southeast Asia. Diode array detector (DAD) with the wavelengths at 307 and 260 nm was used to monitor the conjugated system of protostemonine (2) and maistemonine (4), respectively, whereas evaporative light scattering detector (ELSD) was employed to detect croomine (1), stemoninine (3), neotuberostemonine (5) and tuberostemonine (6), the other four analytes with no or poor chromophores. The assay was validated to be sensitive, precise and accurate, with a detection limit of 3.64–0.04 µg/mL depending on the individual analytes. The overall intra‐ and inter‐day variations were less than 9.3%, and the overall recoveries higher than 91.2%, respectively. The correlation coefficients of the calibration curves were better than 0.996 for all analytes. The newly established method was successfully utilized to determine six major components in the genuine sources of Radix Stemonae: Stemona japonica, S. sessilifolia and S. tuberosa. Significant variations of contents of these components were demonstrated in samples of these three species. This simple, rapid, low‐cost and reliable method is suitable for the routine quality control of herbal medicines containing bioactive components with different structure types such as Radix Stemonae. Copyright © 2007 John Wiley & Sons, Ltd.
Quantification of pramipexole in human plasma by liquid chromatography tandem mass spectrometry using tamsulosin as internal standard
Authors: Nirogi Ramakrishna V. S. , Kandikere Vishwottam , Shrivastava Wishu , Mudigonda Koteshwara , Maurya Santosh , Ajjala Devender 
Published: June 2007

AbstractA high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of pramipexole in human plasma. Following liquid–liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse‐phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H]+ ions, m/z 212/152 for pramipexole and m/z 409/228 for the IS. The method exhibited a linear dynamic range of 200–8000 pg/mL for pramipexole in human plasma. The lower limit of quantification was 200 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 3.5 min for each sample made it possible to analyze more than 200 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2007 John Wiley & Sons, Ltd.
Retracted: Determination of scutellarin isomer, a predominant metabolite of scutellarin, in human plasma by HPLC/tandem mass spectrometry and its application to a pharmacokinetic study in Chinese healthy volunteers
Authors: Xia Chun‐hua , Xiong Yu‐qing , Wang Guang‐ji 
Published: June 2007

AbstractRetraction: The following article from Biomedical Chromatography, “Determination of scutellarin isomer, a predominant metabolite of scutellarin, in human plasma by HPLC/tandem mass spectrometry and its application to the pharmacokinetic study in Chinese healthy volunteers” by Chun‐hua Xia, Yu‐qing Xiong, and Guang‐ji Wang, published online on 21 June 2007 in Wiley Interscience (www.interscience.wiley.com/journal/bmc), has been retracted at the authors' request.
HPLC with diode‐array detection for the simultaneous determination of di(2‐ethylhexyl)phthalate and mono(2‐ethylhexyl)phthalate in seminal plasma
Authors: Mazzeo Pietro , Pasquale Dorina Di , Ruggieri Fabrizio , Fanelli Maria , D'Archivio Angelo Antonio , Carlucci Giuseppe 
Published: June 2007

AbstractA high‐performance liquid chromatographic method for the simultaneous determination of di(2‐ethylhexyl)phthalate (DEHP) and its major metabolite mono(2‐ethylhexyl)phthalate (MEHP) in seminal plasma was developed and validated. The method involves liquid–liquid extraction followed by isocratic reversed‐phase chromatography with diode‐array detection. The recovery, selectivity, linearity, precision and accuracy of the method were evaluated from the analysis of spiked seminal plasma samples. The effect of mobile‐phase composition and pH on the retention of the target analytes was investigated. The limits of detection were 0.010 and 0.015 µg/mL, for DEHP and MEHP, respectively. This method was used to analyze real samples in support of clinical studies on these potential endocrine disruptors. Copyright © 2007 John Wiley & Sons, Ltd.
Determination and pharmacokinetic comparison of rhein in rats after oral dosed with Da‐Cheng‐Qi decoction and Xiao‐Cheng‐Qi decoction
Authors: Tang Wen‐Fu , Huang Xi , Yu Qin , Qin Feng , Wan Mei‐Hua , Wang Yong‐gang , Liang Mao‐Zhi 
Published: June 2007

AbstractDa‐Cheng‐Qi decoction (DCQD) is composed of dahuang, houpu, zhishi and mangxiao, while Xiao‐Cheng‐Qi decoction (XCQD) just contains dahuang, houpu, and zhishi. A validated high‐performance liquid chromatographic (HPLC) method was developed for the determination and pharmacokinetic comparison of rhein in rats. It was performed on a reversed‐phase C18 column (150 × 4.6 mm) with a mobile phase of methanol and 0.2% acetic acid (89:11, v/v) and ultraviolet detection at 254 nm. The assay was linear over the range 30–3000 ng/mL (r2 = 0.9991), with mean percentage recovery of 101.4%. The RSD of intra‐ and inter‐day precision were less than 3.0%, with accuracy in the range 96.8–102.5% for each of the concentrations tested. Following oral administration, rhein plasma levels revealed curves characterized by peaks at 20.5 and 30.1 min corresponding to dosages of 15 mg/kg (XCQD) and 20 mg/kg (DCQD) at mean concentrations of 1184.67 and 1814.27 ng/mL, respectively. The half‐lives of rhein from XCQD and DCQD were 45.3 and 139.6 min, respectively. AUC(0–12h) of rhein after oral dosage with XCQD and DCQD were 3435.68 and 5382.25 µg/L h, respectively. The pharmacokinetic parameters may be used to guide the clinical prescription compatibility of traditional Chinese prescriptions related to dahuang and mangxiao. Copyright © 2007 John Wiley & Sons, Ltd.
Determination of topotecan in human and mouse plasma and in mouse tissue homogenates by reversed‐phase high‐performance liquid chromatography
Authors: Vries Nienke A. de , Ouwehand Mariët , Buckle Tessa , Beijnen Jos H. , van Tellingen Olaf 
Published: June 2007

AbstractA sensitive and selective reversed‐phase high‐performance liquid chromatographic (HPLC) assay has been developed and validated for quantification of total topotecan in human and mouse plasma and in mouse tissue samples. Isocratic separation was achieved on a Zorbax SB‐C18 column and topotecan was monitored fluorimetrically. Two ranges of calibrations curves were used to determine lower levels of topotecan more accurately. Acceptable accuracy and precision was achieved for all matrices. Topotecan was stable upon repeated freeze–thawing for three cycles or storage for 24 h at ambient temperatures in spiked plasma samples and tissue homogenates, except in heart homogenates. In an additional validation experiment in which 14C‐labeled topotecan was administered to mice, the levels of unchanged topotecan were about 80–90% of the total radioactivity in tissue homogenates collected 10 min after drug administration and virtually similar as in plasma samples. However, results in tissue homogenates obtained 4 h post‐drug administration indicated substantial metabolism of topotecan. This assay is suitable for studying the pharmacokinetics and tissue distribution of topotecan in mice. Our results demonstrate the importance of including all tissues of interest for pharmacokinetic studies in the validation procedure. Copyright © 2007 John Wiley & Sons, Ltd.
Anatomical evaluation of the tibial nerve within the popliteal fossa
Authors: Driban Jeffrey B. , Swanik C. Buz , Barbe Mary F. 
Published: June 2007

AbstractThe purpose of this study was to evaluate the inter‐hamstring position (medial to lateral intertendinous position) of the tibial nerve within the popliteal fossa. A descriptive cadaver model study was performed to permit controlled and direct measures of the tibial nerve. Fourteen embalmed lower extremities (8 left, 6 right) from nine cadavers (4 males, 5 females; 84.3 ± 10.7 years of age) were examined. Nine anatomical variables were measured. All measurements, except the diameter of the tibial nerve at the apex of the popliteal fossa, were performed at the level of the femoral condyles, at their widest medial‐to‐lateral point. The tibial nerve's diameter increased as it descended from the apex (3.95 ± 0.50 mm; CI = 2.94–4.96 mm) to the condyles (4.46 ± 0.92 mm; CI = 2.62–6.31 mm). The distance between the semimembranosus tendon and the biceps femoris tendon in the popliteal fossa (the mid‐intertendinous distance) was 48.50 ± 11.50 mm. The location of the tibial nerve between these two tendons was highly variable: 21.45 ± 8.40 mm lateral of the semimembranosus tendon and 22.60 ± 4.90 mm medial of the biceps femoris tendon. Therefore, in 95% of the patients the tibial N could be located within 48.2% of midpoint of the inter‐hamstring distance. Clin. Anat. 20:694–698, 2007. © 2007 Wiley‐Liss, Inc.
Sphenoid sinus: An anatomic and endoscopic study in Asian cadavers
Authors: Tan H.K.K. , Ong Y.K. 
Published: June 2007

AbstractAn anatomic and endoscopic study of 48 cadaveric heads (96 sphenoid sinuses) was undertaken to describe the anatomy of the sphenoid sinus in Asians. Sellar type of sphenoid sinus is the most common, present in 53 out of 96 sides (55%). Forty‐five of the 48 heads had a dominant sphenoid cavity, of which 11 contained vital structures from both sides of the sphenoid sinus. The incidence of accessory septae, carotid artery, optic nerve, maxillary nerve, and vidian nerve bulges were 70.8%, 67.7%, 69.8%, 61.5%, and 64.6%, respectively. There is a significantly higher number of overriding ethmoid sinuses in Asian cadavers (46/96 sides) compared to western studies (P < 0.0005). Seven (15%) of these 46 sides were also Onodi positive. The rest of the overriding ethmoid cells were Onodi negative. Surgeons should be aware of the significantly higher number of overriding posterior ethmoid cells in Asian populations during functional endoscopic sinus surgery (FESS). The optic nerve is at risk during FESS surgery if the sphenoid sinus is sought behind the deepest point of these posterior ethmoid cells. These overriding posterior ethmoid cells may also confuse the unwary surgeon that he has entered the sphenoid sinus where in fact he is still operating in the posterior ethmoid cells. Clin. Anat. 20:745–750, 2007. © 2007 Wiley‐Liss, Inc.
Bony landmarks as an aid for intraoperative facial nerve identification
Authors: Greyling L.M. , Glanvill R. , Boon J.M. , Schabort D. , Meiring J.H. , Pretorius J.P. , van Schoor A. 
Published: June 2007

AbstractIdentification of the facial nerve trunk is essential during surgery of the parotid gland. Numerous landmarks have been researched and used. The relation between the facial nerve to two constant bony landmarks, the tip of the mastoid process and the central point of the transverse process of the atlas was investigated. Forty cadavers were dissected. A preauricular incision exposed the nerve trunk. Bony landmarks were identified and marked. The distance from the nerve trunk to the mastoid process and the atlas was measured. The mean distance between the mastoid process and nerve for the left was 9.18 ± 2.05 mm and for the right, 9.35 ± 1.67 mm. The mean distance between the atlas and the nerve for the left was 14.31 ± 3.59 mm and for the right, 13.76 ± 4.65 mm. Confidence intervals were determined. The importance of the aforementioned data revolves around minimizing the chance of injury to the facial nerve during surgery. The applicability of these landmarks needs to be studied in the clinical setting. Clin. Anat. 20:739–744, 2007. © 2007 Wiley‐Liss, Inc.
Asymmetry of the active nonweightbearing foot and ankle range of motion for dorsiflexion‐plantar flexion and its coupled movements in adults
Authors: Ferrario Virgilio F. , Turci Michela , Lovecchio Nicola , Shirai Yuri F. , Sforza Chiarella 
Published: June 2007

AbstractAsymmetries in ankle range of motion (ROM) have been reported, but often the uninvolved limb is used as a reference in clinical practice. The study wanted to quantify the intraindividual asymmetries in dorsi‐plantar flexion foot and ankle ROM and its coupled foot movements. Active triplanar nonweightbearing ROM of the foot and ankle was recorded in young healthy adults (30 male volunteers, mean age 22.8 years; 35 female volunteers, mean age 23.8 years) using an optoelectronic set‐up. The sagittal plane movement (mean ROM female subjects right side 71.3°, left side 71.4°, P > 0.05; mean ROM male subjects right side 69°, left side 68.9°, P > 0.05; sex difference, P < 0.001) was coupled with frontal (mean ROM female subjects right side 16.6°, left side 14.8°, P > 0.05; male subjects right side 17°, left side 15.3°; P > 0.05; no sex difference) and horizontal (mean ROM female subjects right side 19.6°, left side 18.8°, P < 0.001; male subjects right side 17.6°, left side 16.2°, P < 0.001; sex < 0.001) plane motions. Individual fluctuating asymmetries up to 15° (principal movement), and up to 29° (associated movements) were measured. Overall, 20% of female and 34% of male subjects had principal plane asymmetries >5°, and 50% of the subjects had asymmetries >5° in the associated movements. In young adults, individual asymmetries in ankle joint complex dorsi‐plantar flexion should be taken into account when using the uninvolved, contralateral limb as a reference for clinical examination. Clin. Anat. 20:834–842, 2007. © 2007 Wiley‐Liss, Inc.
Anatomy of successfully separated thoracopagus–omphalopagus conjoined twins
Authors: Thompson Jess L. , Zarroug Abdallah E. , Matsumoto Jane M.S. , Moir Christopher R. 
Published: June 2007

AbstractThe birth of conjoined twins occurs in approximately 1 in 50,000 to 1 in 100,000 deliveries, and the majority are either stillborn or die early in life. Approximately 184 successful separations have been described. The authors describe the preoperative radiologic evaluation of a set of thoracopagus–omphalopagus twins and their successful surgical separation. Preoperative radiologic imaging demonstrated that the twins did not share myocardium, but an ectopic cordis was present. The inferior vena cavae entered the right atria at more acute angles than normal. The liver was fused, two gallbladders were present, and there was a large bridging portal vein that crossed the hepatic isthmus of one twin to the conjoined twin's portal venous system. Replaced hepatic arteries originated from the superior mesenteric arteries. Each twin had separate kidneys, colons, and rectums. Preoperative tissue expansion was necessary, and the twins were separated at 5½ months of life. Successful separation of twins conjoined by several organ systems requires a multispecialty approach. Preoperative knowledge of shared anatomy is necessary for adequate preparation and planning. Despite meticulous preoperative investigations, certain anatomy can only be discovered at the time of surgery, and the ability to adjust to these findings is imperative. Clin. Anat. 20:814–818, 2007. © 2007 Wiley‐Liss, Inc.
Towards a Protocol for Solution Structure Determination of Copper(II) Proteins: the Case of CuIIZnII Superoxide Dismutase
Authors: Bertini Ivano , Felli Isabella C. , Luchinat Claudio , Parigi Giacomo , Pierattelli Roberta 
Published: June 2007

AbstractWe have developed an optimized protocol to solve the solution structure of copper(II) proteins. After assignment, proton–proton NOEs are used for the shell where 1H spectra are conveniently observed. In a shell closer to the metal ion, 13C NMR spectra with band‐selective homonuclear decoupling provide the assignment of all nuclei except for those of the metal ligands. A convenient method for the measurement of 13C longitudinal‐relaxation rates (R1) of carbonyls and carboxylate moieties is proposed. 1H NOEs and 1H and 13C R1 data are sufficient to produce a good/reasonable solution structure, as demonstrated for a monomeric species of superoxide dismutase, a 153‐residue protein.
Chronic total occlusion treatment in post‐CABG patients: Saphenous vein graft versus native vessel recanalization—Long‐term follow‐up in the drug‐eluting stent era
Authors: Meliga Emanuele , García‐García Héctor M. , Kukreja Neville , Daemen Joost , Tanimoto Shuzou , Ramcharitar Steve , van Mieghem Carlos A.G. , Sianos Georgios , van der Ent Martin , van der Giessen Willem J. , de Feyter Pim , van Domburg Ron , Serruys Patrick W. 
Published: June 2007

AbstractObjective:To compare the postprocedural and long‐term clinical outcomes of two groups of patients, all presenting with chronic saphenous vein graft (SVG) occlusion, who underwent either SVG or native vessel reopening.Background:Chronic total occlusions (CTO) treatment in patients who underwent previous surgical revascularization is a dilemma and the choice of performing native vessel or SVG recanalization is not always easy.Methods:Between July 2002 and October 2004, a total of 260 patients were successfully treated for a CTO. Of them, we selected all patients (n = 24) who had previous bypass surgery with graft occlusion. Of this final group, 13 patients underwent a percutaneous graft recanalization while 11 underwent native vessel reopening.Results:Primary end points were in‐hospital and 3‐year rates of death, myocardial infarction, target lesion revascularization, and target vessel revascularization. No events occurred in either group during the in‐hospital period. Cumulative 3‐year event‐free survival in the native vessel and SVG group was 81.8% and 83.9% respectively (P = NS). One death and one TVR occurred in each group.Conclusion:In selected cases, SVG reopening instead of the native vessel is feasible. In such a high‐risk population, drug‐eluting stent implantation in both SVG and native CTO lesions is associated with good long‐term outcomes. © 2007 Wiley‐Liss, Inc.