bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mineralization of bisphenol A by advanced oxidation processes
Authors: Rivas F. Javier , Encinas Ángel , Acedo Benito , Beltrán Fernando J. 
Published: November 2008

AbstractBACKGROUND: Endocrine disruptors, as in the case of bisphenol A (BPA), are increasingly found in aqueous effluents. The degree of mineralization of a bisphenol A (BPA) aqueous solution after applying several oxidation treatments has been investigated.RESULTS: UV‐C photolysis of BPA allowed calculation of the quantum yield, ϕλ=254 = 0.045 ± 0.005 mol Einstein−1 but only 15% of the initial organic carbon content (TOC) was eliminated. Better results (80% conversion) were obtained after TiO2 addition. Ozone inmediately reacts with BPA. Again, TiO2 addition in the presence of O3 was capable of increasing the mineralization level (60%). The photolytic ozonation of BPA was capable of completely eliminating TOC. The presence of activated carbon in the O3/UV and O3/UV/TiO2 systems significantly enhanced the TOC removal reaction rate (100% conversion in 20 min).CONCLUSIONS: Processes such as ozonation or photolysis are capable of efficiently removing BPA from water however, mineralization levels are rather low. Addition of TiO2 to O3 or UV‐C significantly enhances TOC removal. The remaining organics still account for an average 20–40% of the initial organic carbon. The combination of O3/UV‐C is capable of completely mineralizing BPA. Activated carbon and/or TiO2 addition to the system O3/UV‐C improves the TOC depletion rate. Copyright © 2008 Society of Chemical Industry
Perturbation training prior to ACL reconstruction improves gait asymmetries in non‐copers
Authors: Hartigan Erin , Axe Michael J. , Snyder‐Mackler Lynn 
Published: November 2008

AbstractWe investigated whether preoperative perturbation training would help anterior cruciate ligament (ACL) deficient individuals who complain of knee instability (“non‐copers”) regain quadriceps strength and walk normally after ACL reconstruction. Nineteen non‐copers with acute ACL injury were randomly assigned into a perturbation group (PERT) or a strengthening group (STR). The PERT group received specialized neuromuscular training and progressive quadriceps strength training, whereas the STR group received progressive quadriceps strength training only. We compared quadriceps strength indexes and knee excursions during the mid‐stance phase of gait preoperatively to data collected 6 months after ACL reconstruction. Analyses of Variance with repeated measures (time/limb) were conducted to compare quadriceps strength index values over time (time × group) and differences in knee excursions in limbs between groups over time (limb × time × group). If significance was found, post hoc analyses were performed using paired and independent t‐tests. Quadriceps strength indexes before intervention (Pert: 87.2%; Str: 75.8%) improved 6 months after ACL reconstruction in both groups (Pert: 97.1%; Str: 94.4%). Non‐copers who received perturbation training preoperatively had no differences in knee excursions between their limbs 6 months after ACL reconstruction (p = 0.14), whereas those who received just strength training continued to have smaller knee excursions during the mid‐stance phase of gait (p = 0.007). Non‐copers strength and knee excursions were more symmetrical 6 months postoperatively in the group that received perturbation training and progressive quadriceps strength training than the group who received strength training alone. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 724–729, 2009
Host‐derived neoangiogenesis with short‐term immunosuppression allows incorporation and remodeling of vascularized diaphyseal allogeneic rabbit femur transplants
Authors: Giessler Goetz A. , Zobitz Mark , Friedrich Patricia F. , Bishop Allen T. 
Published: November 2008

AbstractThe purpose of this study was to demonstrate that living bone allotransplants can incorporate, remodel, and maintain mechanical properties without long‐term immunosuppression in a fashion comparable to living autotransplants. For this, viability is maintained by repair of nutrient vessels and neovascularization from implanted host‐derived vasculature. Microsurgically revascularized femoral diaphysis allotransplants were transferred from young male New‐Zealand‐White (NZW) into 4 groups of male Dutch‐Belted (DB) rabbits. Short‐term immunosuppression by tacrolimus (IS, groups 4 and 5) and host‐derived neovascularization (NV) from implanted fascial flaps was used to maintain viability (groups 3 and 5) as independent variables. Group 2 received neither IS nor NV. Vascularized pedicled autotransplants were orthotopically transplanted in group 1. After 16 weeks, transplants were evaluated using radiologic, histologic, biomechanical, and histomorphometric parameters. Vascularized bone allotransplants treated with both short‐term IS and host‐derived NV (group 5) healed in a fashion similar to pedicled autotransplants (group 1). Their radiographic scores were higher than other groups. Groups with patent fascial flaps (3 and 5) showed significantly greater neoangiogenesis than ligated controls (2 and 4). Tacrolimus administration did not affect neoangiogenesis. Elastic modulus and ultimate stress were significantly greater in autogenous bone than in allotransplanted femora. Biomechanical properties were not significantly different among allotransplants. Bone turnover was decreased with IS, but increased with NV by the implanted fascial flaps. Living allogeneic femoral allotransplants treated with short‐term IS and host‐derived neoangiogenesis can lead to stable transplant incorporation in this rabbit model. The combination of both factors optimizes bone healing. Transplant mineralization is improved with neoangiogenesis but diminished with IS. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 763–770, 2009
Prostaglandin E2 inhibits BMP signaling and delays chondrocyte maturation
Authors: Clark Christine A. , Li Tian‐Fang , Kim Kyung‐Ok , Drissi Hicham , Zuscik Michael J. , Zhang Xinping , O'Keefe Regis J. 
Published: November 2008

AbstractWhile cyclooxygenases are important in endochondral bone formation during fracture healing, mechanisms involved in prostaglandin E2 (PGE2) regulation of chondrocyte maturation are incompletely understood. The present study was undertaken to determine if PGE2 effects on chondrocyte differentiation are related to modulation of the bone morphogenetic protein (BMP) signaling pathway. In primary murine sternal chondrocytes, PGE2 differentially regulated genes involved in differentiation. PGE2 induced type II collagen and MMP‐13, had minimal effects on alkaline phosphatase, and inhibited the expression of the maturational marker, type X collagen. In BMP‐2–treated cultures, PGE2 blocked the induction of type X collagen. All four EP receptors were expressed in chondrocytes and tended to be inhibited by BMP‐2 treatment. RCJ3.1C5.18 chondrocytes transfected with the protein kinase A (PKA) responsive reporter, CRE‐luciferase, showed luciferase induction following exposure to PGE2, consistent with activation of PKA signaling and the presence of the EP2 and EP4 receptors. Both PGE2 and the PKA agonist, dibutyryl cAMP, blocked the induction of the BMP‐responsive reporter, 12XSBE, by BMP‐2 in RCJ3.1C5.18 chondrocytes. In contrast, PGE2 increased the ability of TGF‐β to activate the TGF‐β‐responsive reporter, 4XSBE. Finally, PGE2 down‐regulated BMP‐mediated phosphorylation of Smads 1, 5, and 8 in RCJ3.1C5.18 cells and in primary murine sternal chondrocytes. Altogether, the findings show that PGE2 regulates chondrocyte maturation in part by targeting BMP/Smad signaling and suggest an important role for PGE2 in endochondral bone formation. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 785–792, 2009
Reproducibility of 31P cardiac magnetic resonance spectroscopy at 3 T
Authors: Tyler D. J. , Emmanuel Y. , Cochlin L. E. , Hudsmith L. E. , Holloway C. J. , Neubauer S. , Clarke K. , Robson M. D. 
Published: November 2008

AbstractThe purpose of this work was to take advantage of the new clinical field strength of 3 T to implement and optimize a chemical shift imaging (CSI) acquisition protocol to produce spectra of high quality with high specificity to the myocardium within a clinically feasible scan time. Further, an analysis method was implemented dependent purely on anatomical location of spectra, and as such free from any potential user bias caused by inference from spectral information. Twenty healthy male subjects were scanned on two separate occasions using the optimized CSI protocol at 3 T. Data were analyzed for intra‐ and inter‐subject variability, as well as intra‐ and inter‐observer variability. The average phosphocreatine (PCr)/adenosine triphosphate (ATP) value for scan 1 was 2.07 ± 0.38 and for scan 2 was 2.14 ± 0.46, showing no significant difference between scans. Intra‐subject variability was 0.43 ± 0.35 (percentage difference 20%) and the inter‐subject coefficient of variation was 18%. The intra‐observer variability, assessed as the absolute difference between analyses of the data by a single observer, was 0.14 ± 0.24 with no significant difference between analyses. The inter‐observer variability showed no significant differences between the PCr/ATP value measured by four different observers as demonstrated by an intra‐class correlation coefficient of 0.763. The increased signal available at 3 T has improved spatial resolution and thereby increased myocardial specificity without any significant decrease in reproducibility over previous studies at 1.5 T. We present an acquisition protocol that routinely provides high quality spectra and a robust analysis method that is free from potential user bias. Copyright © 2008 John Wiley & Sons, Ltd.
High‐resolution MRI of internal field diffusion‐weighting in trabecular bone
Authors: Sigmund E.E. , Cho H. , Song Y.‐Q. 
Published: November 2008

AbstractTrabecular bone structure is known to play a crucial role in the overall strength, and thus fracture risk, of such areas of the skeleton as the vertebrae, spine, femur, tibiae, or radius. Several MR methods devoted to probing this structure depend upon the susceptibility difference between the solid bone matrix and the intervening fluid/marrow/fat, usually in the context of a linewidth (1/T2′) measurement or mapping technique. A recently demonstrated new approach to this system involves using internal gradients to encode diffusion weighting, and extracting structural information (e.g., surface‐to‐volume ratio) from the resulting signal decay. This contrast method has been demonstrated in bulk measurements on cleaned, water‐saturated bovine trabecular bone samples. In the present work, microscopic imaging (0.156 mm in‐plane resolution) is performed in order to spatially resolve this contrast on the trabecular level, and confirm its interpretation for the bulk measurements. It is found that the local rate of decay due to diffusion in the internal field (DDIF) is maximal close to the trabecular surfaces. The overall decay rate in a lower resolution scan probes the abundance of these surfaces, and provides contrast beyond that found in conventional proton density weighted or T1‐weighted imaging. Furthermore, a microscopic calculation of internal field distributions shows a qualitative distinction between the structural sensitivities of DDIF and T2′. DDIF contrast is highly localized around trabecular walls than is the internal field itself, making it a less sensitive but more specific measure of such important properties as trabecular number. Copyright © 2008 John Wiley & Sons, Ltd.
Analysis of the clonal architecture of the human small intestinal epithelium establishes a common stem cell for all lineages and reveals a mechanism for the fixation and spread of mutations
Authors: Gutierrez‐Gonzalez Lydia , Deheragoda Maesha , Elia George , Leedham Simon J , Shankar Arjun , Imber Charles , Jankowski Janusz A , Turnbull Douglass M , Novelli Marco , Wright Nicholas A , McDonald Stuart AC 
Published: November 2008

AbstractLittle is known about the clonal structure or stem cell architecture of the human small intestinal crypt/villus unit, or how mutations spread and become fixed. Using mitochondrial DNA (mtDNA) mutations as a marker of clonal expansion of stem cell progeny, we aimed to provide answers to these questions. Enzyme histochemistry (for cytochrome c oxidase and succinate dehydrogenase) was performed on frozen sections of normal human duodenum. Laser‐capture microdissected cells were taken from crypts/villi. The entire mitochondrial genome was amplified using a nested PCR protocol; sequencing identified mutations and immunohistochemistry demonstrated specific cell lineages. Cytochrome c oxidase‐deficient small bowel crypts were observed within all sections: negative crypts contained the same clonal mutation and all differentiated epithelial lineages were present, indicating a common stem cell origin. Mixed crypts were also detected, confirming the existence of multiple stem cells. We observed crypts where Paneth cells were positive but the rest of the crypt was deficient. We have demonstrated patches of deficient crypts that shared a common mutation, suggesting that they have divided by fission. We have shown that all cells within a small intestinal crypt are derived from one common stem cell. Partially‐mutated crypts revealed some novel features of Paneth cell biology, suggesting that either they are long‐lived or a committed Paneth cell‐specific long‐lived progenitor was present. We have demonstrated that mutations are fixed in the small bowel by fission and this has important implications for adenoma development. Copyright © 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
3q27 aberrations in a childhood ovary teratoma with associated malignant germ cell component
Authors: Brassesco María Sol , Castro‐Gamero Angel Mauricio , Valera Elvis Terci , Neder Luciano , Elias Jorge , Tone Luiz Gonzaga 
Published: November 2008

AbstractCytogenetic studies of childhood ovary tumors have been poorly described. In the present article, the cytogenetic findings of an ovarian teratoma with malignant germ cell (yolk‐sac) component occurring in an 8‐year‐old female are detailed. GTG‐banding showed a karyotype of 46,XX, t(3;20)(q27;q13.3) [4]/46,XX, del3q27 [3]/46,XX [30]. Previous studies have demonstrated common sites of loss of heterozygosity at 3q27‐q28 region in different types of cancer, suggesting the presence of tumor suppressor genes within this region. Pediatr Blood Cancer 2009;52:398–401. © 2008 Wiley‐Liss, Inc.
Mindfulness‐based stress reduction and cancer: a meta‐analysis
Authors: Ledesma Dianne , Kumano Hiroaki 
Published: November 2008

AbstractObjective: This meta‐analysis was conducted to investigate the effects of mindfulness‐based stress reduction (MBSR) on the mental and physical health status of various cancer patients.Methods: Ten studies (randomized‐controlled trials and observational studies) were found to be eligible for meta‐analysis. Individual study results were categorized into mental and physical variables and Cohen's effect size d was computed for each category.Results: MBSR may indeed be helpful for the mental health of cancer patients (Cohen's effect size d=0.48); however, more research is needed to show convincing evidence of the effect on physical health (Cohen's effect size d=0.18).Conclusion: The results suggest that MBSR may improve cancer patients' psychosocial adjustment to their disease. Copyright © 2008 John Wiley & Sons, Ltd.
A peptide‐centric approach to breast cancer biomarker discovery utilizing label‐free multiple reaction monitoring mass spectrometry
Authors: Metodieva Gergana , Greenwood Christina , Alldridge Louise , Sauven Paul , Metodiev Metodi 
Published: November 2008

AbstractWe report an approach for multiplex analysis of cancer biomarkers based on the measurement of diagnostic peptides in whole tissue protein digests. Label‐free quantitation with MS3 multiple reaction monitoring (MRM) was developed to afford accurate analysis of prospective marker peptides in a panel of breast tumors. This approach provides an economical and robust alternative to stable isotope‐based methods. It is equally applicable to the analysis of samples derived from tissue biopsy, aspirate, or plasma and can be easily translated to clinic.
Charting online OMICS resources: A navigational chart for clinical researchers
Authors: Vizcaíno Juan Antonio , Mueller Michael , Hermjakob Henning , Martens Lennart 
Published: November 2008

AbstractThe life sciences have sprouted several popular and successful OMICS technologies that span all levels of biological information transfer. Ever since the start of the Human Genome Project, the then revolutionary idea to make all resulting data publicly available has been central to all of the efforts across OMICS technologies. As a result, a great variety of publicly available data repositories and resources is currently available to the research community. This widespread availability of data does come at the price of increased confusion on the part of the users, especially for those that see the OMICS technologies as tools to help unravel a larger biological or clinical question. We therefore provide a comprehensive overview of the available resources across OMICS fields, with a special emphasis on those databases that are relevant to the study of proteins. Additionally, we also describe various integrative systems that have been established, and highlight new developments in the field that can revolutionize the way in which live data integration is achieved over the internet.
Proteomic analysis of breast cancer tissue reveals upregulation of actin‐remodeling proteins and its relevance to cancer invasiveness
Authors: Kim Dong‐Hyun , Bae Jinhee , Lee Jong Won , Kim Seon‐Young , Kim Yong‐Hak , Bae Ji‐Yeon , Yi Jae Kyo , Yu Myeong‐Hee , Noh Dong‐Young , Lee Cheolju 
Published: November 2008

AbstractThere is an emerging interest in protein expression profiling with the aim of identifying novel diagnostic markers and therapeutic targets in breast cancer. We analyzed breast cancer tissues by 2‐D DIGE using a narrow range IPG strip (pH 5.5–6.7) after the immunodepletion of serum albumin and Ig. Sixty‐three protein spots were detected with more than ±1.8‐fold differences (p <0.05 for three technical replicates) from a set of tissue samples in which three tumor and three nontumor samples were randomly selected from six breast cancer subjects and pooled separately. Of these, 53 proteins were successfully identified by MS. Among the proteins whose levels were increased, we identified three novel WD‐repeat‐motif‐bearing proteins that have been known to be involved in actin remodeling: Arp2/3 complex subunit 2 (p34‐Arc), coronin‐1A and WD‐repeat protein 1 (Wdr1). Significantly increased amounts of p34‐Arc and coronin‐1A in breast cancer were also shown by Western blot analysis of matched tumor and nontumor tissue samples (N = 11, p <0.05), and were consistent with the mRNA levels retrieved from publicly available microarray databases. The siRNA knockdown of p34‐Arc attenuated the invasion of SK‐BR3 breast cancer cells into Matrigel. In contrast, the overexpression of coronin‐1A increased this invasive activity. Taken together, the cellular levels of p34‐Arc and coronin‐1A were linked to cancer development and migration. The data obtained from the present study provides new insight into the management of breast cancer.
Atlas of dihydrotestosterone actions on the transcriptome of prostate in vivo
Authors: Ma Ci , Yoshioka Mayumi , Boivin André , Gan Lin , Takase Yasukazu , Labrie Fernand , St‐Amand Jonny 
Published: November 2008

AbstractBACKGROUNDUsing serial analysis of gene expression (SAGE), we studied the transcriptomic changes in vivo by dihydrotestosterone (DHT) treatment in mice to better understand androgen effects in the prostate.METHODSApproximately 872,000 SAGE tags were isolated from intact and castrated (GDX) mice with and without DHT injection.RESULTSGDX significantly altered 431 transcripts, including 110 transcripts restored by DHT, and 146 potentially new transcripts. Totally, 187 transcripts were significantly affected by DHT treatment, of which 124 were induced and 63 were repressed. Interestingly and consistent with the prostate's secretory role, DHT up‐regulated the expression of many genes involved in various steps of protein metabolism such as synthesis, folding, and secretion. GDX modulated the expression of genes which induce cell apoptosis and inhibit cell proliferation through polyamine biosynthesis, retinoid X receptor actions as well as several signaling pathways and some related factors. These results clarify DHT effects on prostate transcriptome in the areas of protein metabolism, cell proliferation and apoptosis. In addition, we detected gene expression changes in metabolic pathways, cytoskeleton, immunity and endoplasmic reticulum stress. Furthermore, knockdown of S‐adenosylmethionine decarboxylase 1 in LNCaP cells confirmed the importance of androgen‐regulated genes (ARGs) in prostate cancer cell growth.CONCLUSIONOur data support the idea that ARGs are essential for the normal development of the prostate and can also be responsible for the pathogenesis of the prostate cancer. Prostate 69:293–316, 2009. © 2008 Wiley‐Liss, Inc.
Interactive effects of cover and hydraulics on brown trout habitat selection patterns
Authors: Ayllón D. , Almodóvar A. , Nicola G. G. , Elvira B. 
Published: November 2008

AbstractHabitat modelling results are extremely sensitive to the habitat suitability criteria (HSC) used in the simulations. HSCs are usually expressed as univariate habitat suitability curves, although such univariate approach has been long questioned, since overlooking interactions between hydraulic variables may misrepresent the complexity of fish behaviour in habitat selection. It could lead to adopt erroneous flow management decisions based on misleading results. Furthermore, the interactive effects of hydraulic variables on habitat selection may be driven by the structural features of the channel, which determine cover availability. Therefore, we compared brown trout habitat selection patterns through multivariate resource selection functions (RSFs) in structurally contrasting rivers to unveil the interactive effects of hydraulics and cover elements and their consequences in univariate HSC results. Microhabitat preferences of young‐of‐the‐year (0+) trout were similar across fast and slow waters, meanwhile juvenile (1+) and adult (>1+) preferences significantly changed. RSFs for young‐of‐the‐year trout were consistent with univariate results and did not differ among water types. However, RSFs for older trout varied among water types and revealed complex interactions among hydraulic variables and between hydraulics and structural elements, which were not described accurately by univariate curves. Therefore, results suggest that interactions between water depth and current velocity have a significant effect on habitat selection patterns in juvenile and adult brown trout, this effect being controlled by cover availability. Copyright © 2008 John Wiley & Sons, Ltd.