bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insulin‐like growth factor‐I increases bone sialoprotein (BSP) expression through fibroblast growth factor‐2 response element and homeodomain protein‐binding site in the proximal promoter of the BSP gene
Authors: Nakayama Youhei , Nakajima Yu , Kato Naoko , Takai Hideki , Kim Dong‐Soon , Arai Masato , Mezawa Masaru , Araki Shouta , Sodek Jaro , Ogata Yorimasa 
Published: April 2006

AbstractInsulin‐like growth factor‐I (IGF‐I) promotes bone formation by stimulating proliferation and differentiation of osteoblasts. Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. To determine the molecular mechanism of IGF‐I regulation of osteogenesis, we analyzed the effects of IGF‐I on the expression of BSP in osteoblast‐like Saos2 and in rat stromal bone marrow (RBMC‐D8) cells. IGF‐I (50 ng/ml) increased BSP mRNA levels at 12 h in Saos2 cells. In RBMC‐D8 cells, IGF‐I increased BSP mRNA levels at 3 h. From transient transfection assays, a twofold increase in transcription by IGF‐I was observed at 12 h in pLUC3 construct that included the promoter sequence from −116 to +60. Effect of IGF‐I was abrogated by 2‐bp mutations in either the FGF2 response element (FRE) or homeodomain protein‐binding site (HOX). Gel shift analyses showed that IGF‐I increased binding of nuclear proteins to the FRE and HOX elements. Notably, the HOX‐protein complex was supershifted by Smad1 antibody, while the FRE‐protein complex was shifted by Smad1 and Cbfa1 antibodies. Dlx2 and Dlx5 antibodies disrupted the formation of the FRE‐ and HOX‐protein complexes. The IGF‐I effects on the formation of FRE‐protein complexes were abolished by tyrosine kinase inhibitor herbimycin A (HA), PI3‐kinase/Akt inhibitor LY249002, and MAP kinase kinase inhibitor U0126, while IGF‐I effects on HOX‐protein complexes were abolished by HA and LY249002. These studies demonstrate that IGF‐I stimulates BSP transcription by targeting the FRE and HOX elements in the proximal promoter of BSP gene. J. Cell. Physiol. 208: 326–335, 2006. © 2006 Wiley‐Liss, Inc.
Date consumption and dietary significance in the United Arab Emirates
Authors: Ismail Baraem , Henry Jeya , Haffar Imad , Baalbaki Riad 
Published: April 2006

AbstractDate consumption in the United Arab Emirates was determined among different subgroups of the nation's society. Adult consumers of both sexes and different age groups (18–30 and 30–60) and occupations (students, workers, at home) were surveyed for information on their daily consumption of six date food groups (rutab, tamr, seh, debbs, batheeth and madlouj). Percentage distribution of portion size, frequency of consumption and season, over different consumer groups per date food group, were determined. Differences in the daily consumption of every date food group within and among the different population groups were tested. The most consumed date food groups were rutab and tamr, with rutab being more frequently consumed than tamr during the summer season. Overall, no major differences were observed in average daily consumption per capita of most of the date food groups between males and females, between employed and home‐based consumers, and between students and individuals of 18–30 years old. On average, daily consumption of dates per capita was 114.3 g, equivalent to 10 date fruits. However, it was noted that the younger population does not consume dates as much as the older population does. Nevertheless, data obtained highlighted the significance of dates in the UAE nation's diet considering the relatively high average consumption of dates per capita. Copyright © 2006 Society of Chemical Industry
Seligman lecture 2005 food product engineering: building the right structures
Authors: Aguilera José Miguel 
Published: April 2006

AbstractDriven by consumers' expectations and new knowledge, a paradigmatic shift is occurring in food engineering from the prior emphasis in processes and unit operations to the design of products that provide convenience, health and well‐being. The structure of foods affects their sensorial, physical and transport properties as well as the bioavailability of some nutrients. Food structure is provided by nature or imparted during processing and preparation. Presently, food product engineering is stabilizing, transforming and creating edible microstructures that are desired by consumers using conventional unit operations. This paper revises the progress in the science of food materials and its contribution to the understanding of how food structures are formed from the molecular to the macromolecular level. Food product design of the future will be based on a wider scientific knowledge adopted from many disciplines and advanced tools that reduce the scale of fabrication. This poses a challenge to food engineering education. Copyright © 2006 Society of Chemical Industry
Brain 1H magnetic resonance spectroscopic differences in myotonic dystrophy type 2 and type 1
Authors: Vielhaber Stefan , Jakubiczka Sibylle , Gaul Charly , Schoenfeld Mircea Ariel , Debska‐Vielhaber Grazyna , Zierz Stefan , Heinze Hans‐Jochen , Niessen Heiko G. , Kaufmann Jörn 
Published: April 2006

AbstractTo evaluate cerebral metabolism and intergroup differences in closely matched patients with myotonic dystrophy type 2 (DM2, n = 15) and type 1 (DM1, n = 14), we performed 1H magnetic resonance spectroscopic (MRS) analyses of the occipital and temporoparietal cortical regions as well as of subcortical frontal white matter. Relative to healthy subjects, the concentration of N‐acetylaspartate was significantly reduced in all tested brain regions in both disease groups. In the DM1 patients we also observed a concomitant depletion of creatine and choline levels, particularly in the frontal white matter. A discriminant analysis based on the 1H‐MRS data distinguished between the DM2, DM1, and control groups with an overall accuracy of 88%. 1H‐MRS indicates that neurochemical alterations involving gray and white matter occur in patients with DM2 and DM1. Although structural abnormalities (cerebral atrophy, white matter lesions) are similar in DM2 and DM1, changes in cerebral metabolites can differentiate these disease groups, suggesting that the diseases differ in their neurocellular pathology. Muscle Nerve, 2006
New π‐conjugated polyaryleneethynylenes containing a 1,3,5‐triazine unit in the main chain: Synthesis and optical and electrochemical properties
Authors: Fang Qiang , Ren Shijie , Xu Bing , Du Junping , Cao Amin 
Published: April 2006

AbstractA series of new π‐conjugated poly(aryleneethynylene)s containing a 1,3,5‐triazine unit in the main chain were synthesized in yields higher than 70% by polycondensation between dibromophenyl‐1,3,5‐triazine monomers and alkyl‐substituted diethynylbenzene (or diethynylfluorene) comonomers with Pd(PPh3)4 and CuI as catalysts in the presence of triethylamine. The polymers had a number‐average molecular weight in the range of 5000–10,000 and showed good solubility in common organic solvents. The polymers were photoluminescent both in solutions and in the solid state. X‐ray diffraction patterns of the powders of the polymers revealed that the polymers were semicrystalline. Electrochemically, the polymers appeared to be reversible under reduction. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 3797–3806, 2006
Dendritic growth of an elliptical paraboloid with forced convection in the melt
Authors: Ananth Ramagopal , Gill William N. 
Published: April 2006

All experimental observations of the growth of fully developed dendritic ice crystals indicate that the shape of the tip region is an elliptical paraboloid. Therefore, moving-boundary solutions of the three-dimensional Navier-Stokes and energy equations are obtained here for the shape-preserving growth of isothermal elliptical paraboloids by using the Oseen approximation which is valid for the low-Reynolds-number viscous flows which prevail in dendritic growth. Explicit expressions for the flow and the temperature fields are derived in a simple way using Ivantsov's method. It is shown that the growth Péclet number,PG, is a function of the aspect ratioA, the Stefan numberSt, the Reynolds numberRe, and the Prandtl numberPr. As the Reynolds number increasesPGbecomes linear inSt, less dependent onAand ultimately varies roughly asRe½.A comparison between the exact solutions given here and the experiments of Kallungal (1974) indicate thatAdecreases asReincreases. This result agrees qualitatively with the experiments of Kallungal (1974) and Chang (1985). The differences between theory and experiments forRe> 10−3may be due to attachment kinetic resistance to growth along thec-axis and capillary effects at the tip which make ice dendrites non-isothermal and create conduction in the solid phase. However, more accurate simultaneous measurements ofR1andR2are needed to determine definitively the mechanisms responsible for these deviations between theory and experiment.
Resonant growth of three-dimensional modes in trnsitioning Blasius boundary layers
Authors: Corke T. C. , Mangano R. A. 
Published: April 2006

By carefully controlled phase-coupled input of simultaneous two- and three-dimensional disturbances, the nonlinear evolution and breakdown of the laminar flow in a boundary layer was examined. This involved the generation of plane Tollmien–Schlichting waves and pairs of oblique waves so as to promote nearresonance conditions which have been theoretically shown to lead to the rapid development of three-dimensionality in unstble boundary layers. Special emphasis is placed on the two prominent mechanisms, namely resonant-triads of Orr–Sommerfeld modes and the secondary instability of the streamwise periodic flow to spanwise periodic three-dimensional disturbances. The sensitivity of these mechanisms on the amplitudes and wavenumbers of the input disturbances was of special focus.The simultaneous two- and three-dimensional wave generation was accomplished using a spanwise array of line heaters suspended just above the wall at the approximate height of the critical layer in the laminar boundary layer. These were operated to produce, through local heating, time-periodic spanwise-phase-varying velocity perturbations. Of primary emphasis in this paper are conditions obtained by the combined forcing of fundamental plane waves with wavenumbers (α, 0) and pairs of subharmonic oblique waves (½α, ± β). The reslults document resonant growth of energy in the subharmonic modes, the formation of staggered lambda vortex patterns with a cross-stream scale commensurate with the seeded ± β condition, and their subsequet transition to turbulence. Complete documentation of the flow field at these various stages is presented using smoke-wire flow visualization and through phase-conditioned hot-wire surveys measuring all three velocity components in three space dimensions.
Response of a turbulent boundary layer to sinusoidal free-stream unsteadiness
Authors: Brereton G. J. , Reynolds W. C. , Jayaraman R. 
Published: April 2006

In this paper, selected findings of a detailed experimental investigation are reported concerning the effects of forced free-stream unsteadiness on a turbulent boundary layer. The forced unsteadiness was sinusoidal and was superimposed locally on an otherwise-steady mainstream, beyond a turbulent boundary layer which had developed under constant-pressure conditions. Within the region over which free-stream unsteadiness was induced, the sinusoidal variation in pressure gradient was between extremes of zero and a positive value, with a positive average level. The local response of the boundary layer to these free-stream effects was studied through simultaneous measurements of the u- and v-components of the velocity fieldAlthough extensive studies of unsteady, turbulent, fully-developed pipe and channel flow have been carried out, the problem of a developing turbulent boundary layer and its response to forced free-stream unsteadiness has received comparatively little attention. The present study is intended to redress this imbalance and, when contrasted with other studies of unsteady turbulent boundary layers, is unique in that: (i) it features an appreciable amplitude of mainstream modulation at a large number of frequencies of forced unsteadiness, (ii) its measurements are both detailed and of high spatial resolution, so that the near-wall behaviour of the flow can be discerned, and (iii) it allows local modulation of the mainstream beyond a turbulent boundary layer which has developed under the well-known conditions of steady, two-dimensional, constant-pressure flowResults are reported which allow comparison of the behaviour of boundary layers under the same mean external conditions, but with different time dependence in their free-stream velocities. These time dependences correspond to: (i) steady flow, (ii) quasi-steadily varying flow, and (iii) unsteady flow at different frequencies of mainstream unsteadiness. Experimental results focus upon the time-averaged nature of the flow; they indicate that the mean structure of the turbulent boundary layer is sufficiently robust that the imposition of free-stream unsteadiness results only in minor differences relative to the mean character of the steady flow, even at frequencies for which the momentary condition of the flow departs substantially from its quasi-steady state. Mean levels of turbulence production are likewise unaffected by free-stream unsteadiness and temporal production of turbulence appears to result only from modulation of the motions which contribute to turbulence production as a time-averaged measure.
Two-dimensional flow of a viscous fluid in a channel with porous walls
Authors: Cox Stephen M. 
Published: April 2006

We consider the flow of a viscous incompressible fluid in a parallel-walled channel, driven by steady uniform suction through the porous channel walls. A similarity transformation reduces the Navier-Stokes equations to a single partial differential equation (PDE) for the stream function, with two-point boundary conditions. We discuss the bifurcations of the steady solutions first, and show how a pitchfork bifurcation is unfolded when a symmetry of the problem is broken.Then we describe time-dependent solutions of the governing PDE, which we calculate numerically. We analyse these unsteady solutions when there is a high rate of suction through one wall, and the other wall is impermeable: there is a limit cycle composed of an explosive phase of inviscid growth, and a slow viscous decay. The inviscid phase ‘almost’ has a finite-time singularity. We discuss whether solutions of the governing PDE, which are exact solutions of the Navier-Stokes equations, may develop mathematical singularities in a finite time.When the rates of suction at the two walls are equal so that the problem is symmetrical, there is an abrupt transition to chaos, a ‘homoclinic explosion’, in the time-dependent solutions as the Reynolds number is increased. We unfold this transition by perturbing the symmetry, and compare direct numerical integrations of the governing PDE with a recent theory for ‘Lorenz-like’ dynamical systems. The chaos is found to be very sensitive to symmetry breaking.
The release of air bubbles from an underwater nozzle
Authors: Longuet-Higgins Michael S. , Kerman Bryan R. , Lunde Knud 
Published: April 2006

Air bubbles released from an underwater nozzle emit an acoustical pulse which is of interest both for the study of bubble detachment and for elucidating the mechanism of sound generation by a newly formed bubble. In this paper we calculate theoretically the sequence of bubble shapes from a given nozzle and show that there is for each nozzle a bubble of maximum volume vmax Assuming that the bubble becomes detached at its ‘neck’, and that the volume of the detached bubble equals the volume V* of the undetached bubble above its ’neck’, we determine for each nozzle diameter D an acoustic frequency f* corresponding to 'slow’ bubble release.Experiments show that the acoustic frequency, hence the bubble size, depends on the rate of air.flow to the bubble, but for slow rates of flow the frequency f is very close to the theoretical frequency f*.High-speed photographs suggest that when the bubble pinches off. the limiting form of the surface is almost a cone. This is accounted for by assuming a line sink along the axis of symmetry. Immediately following pinch-off there is evidence of the formation of an axial jet going upwards into the bubble. This may play a part in stimulating the emission of sound.
Trapped modes in open channels
Authors: Evans D. V. , Linton C. M. 
Published: April 2006

Trapped or edge-wave modes are well-known in linear water-wave theory. They occur at discrete frequencies below a certain cutoff frequency and consist of local oscillations trapped near a long horizontal submerged body in finite or infinite depth or over a sloping beach. Less well known is the existence of trapped modes in certain problems in acoustics where the governing equation is the Helmholtz equation. Jones (1953) has proved the existence of such modes which correspond to point-eigenvalues of the spectrum of the differential operator satisfying certain boundary conditions in a semi-infinite region. In this paper we describe a constructive method for determining point-eigenvalues or trapped-mode frequencies in two specific problems in which the two-imensional Helmholtz equation is satisfied.The problems arise from a consideration of the fluid motion in a long narrow wave tank with a free water surface which contains a vertical cylinder of uniform horizontal cross-section extending throughout the water depth. Separation of the depth dependence results in Helmholtz's equation with Neumann boundary conditions. By seeking solutions which are antisymmetric with respect to the centreline of the channel, trapped modes are constructed for the case of a cylinder of rectangular cross-section placed symmetrically in the centre of the channel and also for the case of a symmetric rectangular indentation in the tank walls. These problems do not appear to be covered directly by Jones’ theory and whilst the method described provides convincing numerical evidence, it falls short of a rigorous existence proof. Extensions to other purely acoustic problems having no water-wave interpretation, including problems which are covered by the general theory of Jones, are also discussed.
Soy Protein Infant Formulae and Follow‐On Formulae
Authors:   ESPGHAN Committee on Nutrition:, Agostoni Carlo , Axelsson Irene , Goulet Olivier , Koletzko Berthold , Michaelsen Kim Fleischerm , Puntis John , Rieu Daniel , Rigo Jacques , Shamir Raanan , Szajewska Hania , Turck Dominique 
Published: April 2006

ABSTRACT:This comment by the European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) Committee on Nutrition summarizes available information on the composition and use of soy protein formulae as substitutes for breastfeeding and cows' milk protein formulae as well as on their suitability and safety for supporting adequate growth and development in infants. Soy is a source of protein that is inferior to cows' milk, with a lower digestibility and bioavailability as well as a lower methionine content. For soy protein infant formulae, only protein isolates can be used, and minimum protein content required in the current European Union legislation is higher than that of cows' milk protein infant formulae (2.25 g/100 kcal vs. 1.8 g/100kcal). Soy protein formulae can be used for feeding term infants, but they have no nutritional advantage over cows' milk protein formulae and contain high concentrations of phytate, aluminum, and phytoestrogens (isoflavones), which might have untoward effects. There are no data to support the use of soy protein formulae in preterm infants. Indications for soy protein formulae include severe persistent lactose intolerance, galactosemia, and ethical considerations (e.g., vegan concepts). Soy protein formulae have no role in the prevention of allergic diseases and should not be used in infants with food allergy during the first 6 months of life. If soy protein formulae are considered for therapeutic use in food allergy after the age of 6 months because of their lower cost and better acceptance, tolerance to soy protein should first be established byclinical challenge. There is no evidence supporting the use of soy protein formulae for the prevention or management of infantile colic, regurgitation, or prolonged crying.
Probing the collective vibrational dynamics of a protein in liquid water by terahertz absorption spectroscopy
Authors: Xu Jing , Plaxco Kevin W. , Allen S. James 
Published: April 2006

AbstractBiological polymers are expected to exhibit functionally relevant, global, and subglobal collective modes in the terahertz (THz) frequency range (i.e., picosecond timescale). In an effort to monitor these collective motions, we have experimentally determined the absorption spectrum of solvated bovine serum albumin (BSA) from 0.3 to 3.72 THz (10–124 cm−1). We successfully extract the terahertz molar absorption of the solvated BSA from the much stronger attenuation of water and observe in the solvated protein a dense, overlapping spectrum of vibrational modes that increases monotonically with increasing frequency. We see no evidence of distinct, strong, spectral features, suggesting that no specific collective vibrations dominate the protein's spectrum of motions, consistent with the predictions of molecular dynamics simulations and normal mode analyses of a range of small proteins. The shape of the observed spectrum resembles the ideal quadratic spectral density expected for a disordered ionic solid, indicating that the terahertz normal mode density of the solvated BSA may be modeled, to first order, as that of a three‐dimensional elastic nanoparticle with an aperiodic charge distribution. Nevertheless, there are important detailed departures from that of a disordered inorganic solid or the normal mode densities predicted for several smaller proteins. These departures are presumably the spectral features arising from the unique molecular details of the solvated BSA. The techniques used here and measurements have the potential to experimentally confront theoretical calculations on a frequency scale that is important for macromolecular motions in a biologically relevant water environment.
Ligand binding by TPR domains
Authors: Cortajarena Aitziber L. , Regan Lynne 
Published: April 2006

AbstractTetratricopeptide repeat (TPR) domains bind specific peptide ligands and are thought to mediate protein–protein interactions in a variety of biological systems. Here we compare peptide ligand‐binding by several different TPR domains. We present specific examples that demonstrate that TPR domains typically undergo little or no structural rearrangement upon ligand binding. Our data suggest that, contrary to a recent proposal, coupled folding and binding is not the common mechanism of ligand recognition by TPR domains.
Betting the Future Online
Authors: Williams Leighton Vaughan 
Published: April 2006

Abstract
The Internet has brought, among other things, the online betting exchange. From desks in the City or from laptops on sunny beaches, those in the know or out of it can place bets with each other on anything under the sun, whether likely—Australia beating England at cricket—or the reverse. If you have a hunch that a genetically engineered Tyrannosaur will wander down Oxford Street next year, and if you want to put money on it, Internet betting exchanges will find someone to accommodate you. Leighton Vaughan Williams explains how a simple change in tax regime has brought something other than just a more efficient gambling system. It has created a strangely accurate way of foretelling the future.