bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘It's quite a balancing act’: A qualitative study of parents' experiences and information needs related to the COVID‐19 pandemic
Authors: Hartling Lisa , Elliott Sarah A. , Wright Kelsey S. , Knisley Lisa , Scott Shannon D. 
Published: February 2024

AbstractIntroductionThroughout the coronavirus disease 2019 (COVID‐19) pandemic, parents and children faced significant challenges as a result of prevention measures implemented to control the spread of the disease. Ensuring that families have access to essential health information is critical for improving health outcomes and adherence to public health recommendations. Understanding parents' experiences and information needs related to the pandemic and associated health measures (e.g., vaccination, mask wearing, social distancing, etc.) will inform the development and dissemination of resources tailored to parents' needs to support informed decision making.MethodsWe conducted a qualitative descriptive study. Between September and November 2021, parents across Canada were recruited online via social media and community organisation newsletters and listservs to participate in focus groups via Zoom. Focus groups were audio‐recorded and transcribed verbatim. Data were coded and analysed using thematic analysis. Participants completed a demographic questionnaire before the focus groups (via SimpleSurveys).ResultsSixty‐seven parents participated in 12 focus groups between October and November 2021. In relation to experiences, parents felt they were (1) constantly trying to balance everything, and (2) trying to do their best with the information they had at the time when making decisions. Regarding information needs, parents reflected on (1) how difficult it was navigating copious amounts of changing information and finding credible sources to rely on, (2) the need for resources that were easily accessible, credible and in plain language and (3) the need for resources that were tailored to their needs to support them and their children make informed decisions.ConclusionsTrying to mitigate the risk of COVID‐19 infection and adhere to public health recommendations, while balancing various factors (work, online learning, and social interactions) and navigating changing information, was overwhelming for many parents. Reflecting on their needs, parents suggested tailored resources that provided concise, credible information in plain language to help them make informed decisions and navigate conflicting information. These findings reveal important knowledge gaps and highlight areas that need to be addressed to support parents during the pandemic period and beyond.Patient or Public ContributionMembers of our established Paediatric Parent Advisory Group (P‐PAG) were involved as collaborators throughout the planning (grant proposal), development and execution of the study. P‐PAG members gave input on the design of the questionnaire, interview guide, recruitment strategy and interpretation of findings.
A systematic review of Radiesse® (calcium hydroxylapatite): evidence and recommendations for the body
Authors: Galadari Hassan , Guida Stefania 
Published: February 2024

AbstractA filler composed of calcium hydroxylapatite and carboxymethylcellulose gel (CaHA/CMC) has been shown to possess biostimulatory and skin‐tightening properties that have been applied not just to the face but also to the body to boost rejuvenation. Recently, evidence and recommendations for CaHA/CMC treatment of the face have been provided, but they are still lacking in terms of body treatment. This article aims to perform a systematic review of the literature on CaHA/CMC applications on the body and provide evidence from the literature. Currently, the only FDA‐approved indication on the body is treating the hands and a recent European Union Medical Device Regulation approval has been released for decolletage treatment. The application of hyperdilutions on the neck, feet, arms, thighs, abdomen, and other body parts has been proven to provide a skin‐tightening effect. However, despite their wide use with a good safety profile in clinical practice, recent trends and guidelines of diluted and hyperdiluted CaHA/CMC have yet to be supported by randomized controlled trials.
PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay: A highly sensitive method for detection of MYD88 L265P mutation
Authors: Viscovo Marcello , Clemmensen Mia de Laurent , Fosso Federica , Maiolo Elena , Autore Francesco , Laurenti Luca , Hohaus Stefan , Chiusolo Patrizia 
Published: February 2024

AbstractIntroductionAgarose gel‐based conventional and real‐time allele‐specific polymerase chain reaction (AS‐PCR) assays are currently used for sensitive detection and quantification of MYD88 L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele‐specific quantification PCR (AS‐qPCR) assay, PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay, that uses Intercalating Nucleic Acid (INA®) technology for increased affinity and specificity.MethodsThis study compares PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay with conventional AS‐PCR. We included a total of 102 peripheral and bone marrow blood samples from 94 patients with a lymphoproliferative disorder. Droplet digital PCR (ddPCR) was used as a third method in case of discrepancy.ResultsA positive percent agreement of 100% (95% CI 0.92–1.0) and a negative percent agreement of 98% (95% CI 0.90–1.0) were found between the conventional AS‐PCR and the AS‐qPCR methods. Including the ddPCR results to validate the discrepant cases, the sensitivity and specificity of PlentiPlex™ MYD88 Waldenström lymphoma qPCR Assay were 1.0 (95% CI 0.97–1.0) and 1.0 (95% CI 0.96–1.0), respectively.ConclusionOur data demonstrate that PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay is a fast, highly sensitive, and specific method for the detection of MYD88 L265P compared with conventional AS‐PCR.
Access to social rights under tension: The growing role of third‐party actors in dealing with the administrative burden and its implications
Authors: Rode Antoine 
Published: February 2024

AbstractThe digital transformation in the public sector is a converging trend in many social protection systems. In France, it is being combined with the gradual closure of many government offices, particularly those responsible for managing social security benefits. This article focuses on one of the consequences of these developments. They lead to an increase in requests for support and help from individuals experiencing administrative burdens. They make their requests to a range of very different local actors, who do not always have the skills or the responsibility to respond to them. Based on a study carried out in social centres in France, this article presents the role of third parties in reducing the administrative burden. More specifically, it investigates the types of costs associated with the administrative burden to which social centres have to respond, and who meets them. The article analyses the tensions posed by this growing role, which range from professional and organisational to political.
From hype to insight: Exploring ChatGPT's early footprint in education via altmetrics and bibliometrics
Authors: Wong Lung‐Hsiang , Park Hyejin , Looi Chee‐Kit 
Published: February 2024

AbstractBackgroundThe emergence of ChatGPT in the education literature represents a transformative phase in educational technology research, marked by a surge in publications driven by initial research interest in new topics and media hype. While these publications highlight ChatGPT's potential in education, concerns arise regarding their quality, methodology, and uniqueness.ObjectiveOur study employs unconventional methods by combining altmetrics and bibliometrics to explore ChatGPT in education comprehensively.MethodsTwo scholarly databases, Web of Science and Altmetric, were adopted to retrieve publications with citations and those mentioned on social media, respectively. We used a search query, “ChatGPT,” and set the publication date between November 30th, 2022, and August 31st, 2023. Both datasets were within the education‐related domains. Through a filtering process, we identified three publication categories: 49 papers with both altmetrics and citations, 60 with altmetrics only, and 66 with citations only. Descriptive statistical analysis was conducted on all three lists of papers, further dividing the entire collection into three distinct periods. All the selected papers underwent detailed coding regarding open access, paper types, subject domains, and learner levels. Furthermore, we analysed the keywords occurring and visualized clusters of the co‐occurring keywords.Results and ConclusionsAn intriguing finding is the significant correlation between media/social media mentions and academic citations in ChatGPT in education papers, underscoring the transformative potential of ChatGPT and the urgency of its incorporation into practice. Our keyword analysis also reveals distinctions between the themes of the papers that received both mentions and citations and those that received only citations but no mentions. Additionally, we noticed a limitation that authors' choice of keywords might be influenced by individual subjective judgements, potentially skewing results in thematic analysis based solely on author‐assigned keywords such as keyword co‐occurrence analysis. Henceforth, we advocate for developing a standardized keyword taxonomy in the educational technology field and integrating Large Language Models to enhance keyword analysis in altmetric and bibliometric tools. This study reveals that ChatGPT in education literature is evolving from rapid publication to rigorous research.
Integrated analysis of single‐cell RNA‐seq and bulk RNA‐seq unravels the molecular feature of M2 macrophages of head and neck squamous cell carcinoma
Authors: Wu Siyuan , Lv Xiaozhi , Wei Haigang , Wu Jialin , Liu Shiwei , Li Xia , Song Jing , Zou Chen , Ai Yilong 
Published: February 2024

AbstractThe connection between head and neck squamous cell carcinoma (HNSC) and M2 tumour‐associated macrophages is not yet fully understood. We gathered gene expression profiles and clinical data from HNSC patients in the TCGA database. Using Consensus Clustering, we categorized these patients into M2 macrophage‐related clusters. We developed a M2 macrophage‐related signature (MRS) through statistical analyses. Additionally, we assessed gene expression in HNSC cells using single‐cell sequencing data (GSE139324). We identified three distinct M2 macrophage‐related clusters in HNSC, each with different prognostic outcomes and immune characteristics. Patients with different MRS profiles exhibited variations in immune infiltration, genetic mutations and prognosis. FCGR2A may play a role in creating an immunosuppressive tumour microenvironment and could potentially serve as a therapeutic target for HNSC. Our study demonstrated that M2 macrophage‐related genes significantly impact the development and progression of HNSC. The M2 macrophage‐related model offered a more comprehensive assessment of HNSC patient prognosis, genetic mutations and immune features. FCGR2A was implicated in immunosuppressive microenvironments and may hold promise for the development of novel immunotherapeutic strategies for HNSC.
LIGHT protein: A novel gingival crevicular fluid biomarker associated with increased probing depth after periodontal surgery
Authors: Esberg Anders , Kindstedt Elin , Isehed Catrine , Lindquist Susanne , Holmlund Anders , Lundberg Pernilla 
Published: February 2024

AbstractAimTo evaluate the protein profiles in gingival crevicular fluid (GCF) in relation to clinical outcomes after periodontal surgery and examine if any selected proteins affect the mRNA expression of pro‐inflammatory cytokines in human gingival fibroblasts.Materials and MethodsThis exploratory study included 21 consecutive patients with periodontitis. GCF was collected, and the protein pattern (n = 92) and clinical parameters were evaluated prior to surgery and 3, 6 and 12 months after surgery. Fibroblastic gene expression was analysed by real‐time quantitative polymerase chain reaction.ResultsSurgical treatment reduced periodontal pocket depth (PPD) and changed the GCF protein pattern. Twelve months after surgery, 17% of the pockets showed an increase in PPD. Levels of a number of proteins in the GCF decreased after surgical treatment but increased with early signs of tissue destruction, with LIGHT being one of the proteins that showed the strongest association. Furthermore, LIGHT up‐regulated the mRNA expression of pro‐inflammatory cytokines interleukin (IL)‐6, IL‐8 and MMP9 in human gingival fibroblasts.ConclusionsLIGHT can potentially detect subjects at high risk of periodontitis recurrence after surgical treatment. Moreover, LIGHT induces the expression of inflammatory cytokines and tissue‐degrading enzymes in gingival fibroblasts.
Maternal birth weight as an indicator of early and late gestational diabetes mellitus: The Japan Environment and Children's Study
Authors: Tagami Kazuma , Iwama Noriyuki , Hamada Hirotaka , Tomita Hasumi , Kudo Rie , Kumagai Natsumi , Wang Hongxin , Izumi Seiya , Watanabe Zen , Ishikuro Mami , Obara Taku , Tatsuta Nozomi , Metoki Hirohito , Ota Chiharu , Sugiyama Takashi , Kuriyama Shinichi , Arima Takahiro , Yaegashi Nobuo , Saito Masatoshi ,   The Japan Environment and Children's Study Group
Published: February 2024

ABSTRACTAimsThis study aimed to investigate the association of maternal birth weight (MBW) with early and late gestational diabetes mellitus (GDM).MethodsA total of 69318 pregnant Japanese women were included in this birth cohort study. The associations between maternal birth weight and early gestational diabetes mellitus (diagnosed at <24 gestational weeks) and late GDM (diagnosed at ≥24 gestational weeks) were investigated using a multinomial logistic regression model, with an maternal birth weight of 3000–3499 g as the reference category.ResultsLower maternal birth weight was associated with higher odds of developing early and late gestational diabetes mellitus (P < 0.0001 and P < 0.0001, respectively). The adjusted odds ratios (aORs) for early gestational diabetes mellitus in participants with a MBW of <2500 g and 2500–2999 g were 1.345 (95% confidence interval [CI]: 0.912–1.984) and 1.338 (95% CI: 1.098–1.629), respectively. The aORs for late gestational diabetes mellitus in participants with a MBW of <2500 g and 2500–2999 g were, 1.657 (95% CI: 1.298–2.115) and 1.218 (95% CI: 1.058–1.402), respectively.ConclusionsRegardless of the gestational age when gestational diabetes mellitus was diagnosed, a lower maternal birth weight was associated with an increased risk of gestational diabetes mellitus. Furthermore, the association of a MBW <2500 g with late gestational diabetes mellitus tended to be stronger than that with early gestational diabetes mellitus.
Antibacterial activity in egg samples from small‐spotted catshark Scyliorhinus canicula and nursehound Scyliorhinus stellaris: A preliminary investigation
Authors: Martinengo Elena , Micarelli Primo , Reinero Francesca Romana , Bertelloni Fabrizio , Fratini Filippo 
Published: February 2024

AbstractThe study aims to identify antibacterial properties in unfertilized eggs of Scyliorhinus canicula and Scyliorhinus stellaris. Despite challenging marine conditions, these eggs retain their integrity for extended periods and remain unaffected by pathogens. The antibacterial activity was measured using minimum inhibitory and minimum bactericidal concentration analysis. The eggs of S. stellaris exhibited a slight inhibitory effect against Staphylococcus aureus and Listeria monocytogenes, whereas both species' eggs showed no activity against gram‐negative microorganisms.
Feasibility of new‐generation endocytoscopy for the real‐time diagnosis of ampullary lesions: A pilot study (with video)
Authors: Han Ming‐Lun , Wang Wen‐Lun , Lee Ching‐Tai , Hsieh Min‐Shu , Tsai Ming‐Chang , Chan Yi‐Chung , Chu Yu‐Long , Wang Hsiu‐Po 
Published: February 2024

AbstractBackground and AimAn early and accurate diagnosis of ampullary neoplasia is crucial; however, sampling bias is still a major concern. New‐generation endocytoscopy enables real‐time visualization of cellular structures and enables an accurate pathological prediction; however, its feasibility for small ampullary lesions has never been investigated.MethodsWe developed a novel endocytoscopic (EC) classification system for ampullary lesions after an expert review and agreement from five experienced endoscopists and one pathologist. We then consecutively enrolled a total of 43 patients with an enlarged ampulla (< 3 cm), all of whom received an endocytoscopic examination. The feasibility of endocytoscopy was evaluated, and the performance of the EC classification system was then correlated with the final histopathology.ResultsIn five cases (11.6%), the endocytoscope could not approach the ampulla, and these cases were defined as technical failure. Among the remaining 38 patients, 8 had histopathology‐confirmed adenocarcinoma, 15 had adenoma, and 15 had non‐neoplastic lesions. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the EC classification system to diagnose ampullary neoplasias were 95.7%, 86.7%, 91.7%, 92.9%, and 92.1%, respectively. Moreover, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the EC classification to diagnose ampullary cancer were 62.5%, 100%, 100%, 90.9%, and 92.1%, respectively. One case with intra‐ampullary papillary–tubular carcinoma was classified as having a non‐neoplastic lesion by endocytoscopy.ConclusionsEndocytoscopy and the novel EC classification system demonstrated good feasibility to discriminate ampullary neoplasias from non‐neoplastic lesions and may be useful for optical biopsies of clinically suspicious ampullary lesions.
miRNAs in cerebrospinal fluid associated with Alzheimer's disease: A systematic review and pathway analysis using a data mining and machine learning approach
Authors: Pereira Jessica Diniz , Teixeira Lívia Cristina Ribeiro , Mamede Izabela , Alves Michelle Teodoro , Caramelli Paulo , Luizon Marcelo Rizzatti , Veloso Adriano Alonso , Gomes Karina Braga 
Published: February 2024

AbstractAlzheimer's disease (AD) is the most common type and accounts for 60%–70% of the reported cases of dementia. MicroRNAs (miRNAs) are small non‐coding RNAs that play a crucial role in gene expression regulation. Although the diagnosis of AD is primarily clinical, several miRNAs have been associated with AD and considered as potential markers for diagnosis and progression of AD. We sought to match AD‐related miRNAs in cerebrospinal fluid (CSF) found in the GeoDataSets, evaluated by machine learning, with miRNAs listed in a systematic review, and a pathway analysis. Using machine learning approaches, we identified most differentially expressed miRNAs in Gene Expression Omnibus (GEO), which were validated by the systematic review, using the acronym PECO—Population (P): Patients with AD, Exposure (E): expression of miRNAs, Comparison (C): Healthy individuals, and Objective (O): miRNAs differentially expressed in CSF. Additionally, pathway enrichment analysis was performed to identify the main pathways involving at least four miRNAs selected. Four miRNAs were identified for differentiating between patients with and without AD in machine learning combined to systematic review, and followed the pathways analysis: miRNA‐30a‐3p, miRNA‐193a‐5p, miRNA‐143‐3p, miRNA‐145‐5p. The pathways epidermal growth factor, MAPK, TGF‐beta and ATM‐dependent DNA damage response, were regulated by these miRNAs, but only the MAPK pathway presented higher relevance after a randomic pathway analysis. These findings have the potential to assist in the development of diagnostic tests for AD using miRNAs as biomarkers, as well as provide understanding of the relationship between different pathophysiological mechanisms of AD.image
Relationship between syringomyelia and myxomatous mitral valve disease in Cavalier King Charles spaniels
Authors: Bach Maiken B. T. , Stougaard Camilla L. , Thøfner Maria S. , Reimann Maria J. , Westrup Ulrik , Koch Jørgen , Fredholm Merete , Martinussen Torben , Berendt Mette , Olsen Lisbeth H. 
Published: February 2024

AbstractBackgroundSyringomyelia (SM) and myxomatous mitral valve disease (MMVD) are highly prevalent in Cavalier King Charles spaniels (CKCS). Cardiac status in CKCS with and without SM is currently unknown.ObjectivesTo investigate the association between SM and MMVD severity in CKCS and CKCS with SM with and without clinical signs of SM.AnimalsFifty‐five CKCS: 40 with SM (22 symptomatic and 18 asymptomatic) and 15 without SM.MethodsA combined retrospective and prospective study. MRI and echocardiography were used to diagnose SM and MMVD, respectively. The association between SM and MMVD severity (left ventricle internal diameter in diastole normalized to bodyweight [LVIDDN] and left atrium to aortic ratio [LA/Ao]) were tested using multivariable linear regression analysis adjusting for sex and age.ResultsOverall, no significant difference in LVIDDN and LA/Ao was found between CKCS with or without SM. However, CKCS with symptomatic SM had significantly smaller LVIDDN (1.45 [1.30‐1.50]) (median [IQR]) and LA/Ao (1.20 [1.10‐1.28]) compared to CKCS with asymptomatic SM (1.60 [1.50‐1.90] and 1.40 [1.20‐1.75]) as well as CKCS without SM (0.24 [0.03‐0.45] and 0.30 [0.05‐0.56]) (all P values <.03).Conclusions and Clinical ImportanceAn association between MMVD and SM was not confirmed in this cohort of CKCS, indicating that MMVD and SM do not co‐segregate. However, CKCS with symptomatic SM had smaller left ventricle and atrial size compared to CKCS with asymptomatic SM and CKCS without SM.