bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proliferative response of T cells from tumor‐immune chickens to carcinogen‐induced fibrosarcoma cells
Authors: Quere Pascale , Tsiagbe Vincent K. , Thorbecke G. Jeanette 
Published: February 2007

AbstractImmunity to carcinogen‐induced transplantable fibrosarcomas (CHCT‐NYU‐4, ‐97, ‐36 and ‐20) in SC chickens was studied by the ability of spleen cells from NYU‐4 or ‐97 immune chickens to proliferate in response to tumor cells in vitro. Spleen, but not peripheral blood cells, from NYU‐4 immune chickens proliferated significantly more vigorously to gamma‐irradiated NYU‐4 cells than did cells from normal chickens. The proliferative response was not much affected by addition of indomethacin. Spleen cells from NYU‐4‐immune agammaglobulinemic (Aγ) chickens exhibit the same ability to proliferate in presence of gamma‐irradiated NYU‐4 tumor cells. Analysis of the phenotype of the T‐cell component involved in proliferation showed that the proliferative response was significantly decreased by removal of CT4+ cells through indirect panning. Removal of CT8+ cells enhanced background proliferation without affecting the total thymidine incorporation in the presence of tumor cells. Immune spleen cells usually gave highest responses to the immunizing tumor, but also exhibited cross‐reactivity to cells from other individual tumors induced by the same carcinogen.
In vitro therapeutic targeting of neuroblastomas using 125I‐labelled meta‐iodobenzylguanidine
Authors: Guerreau Dominique , Thedrez Philippe , Fritsch Paul , Saccavini Jean‐Claude , Metivier Henri , Nolibe Daniel , Masse Roland , Coornaert Sabine , Chatal Jean‐François 
Published: February 2007

AbstractThe use of labelled radiopharmaceuticals such as metaiodobenzylguanidine (m‐IBG) enables neuroblastomas and other malignant cells from neural crests to be visualized. In vitro study of cellular incorporation into human neuroblastoma lines (SK‐N‐SH, SK‐N‐MC, LAN I) showed that only the SK‐N‐SH line retained iodine‐125 m‐IBG (125I‐m‐IBG) significantly. Fifty‐five percent of the initial activity was retained after 1 hr incubation at a concentration of 10−7 M of m‐IBG (specific activity: 1,480 MBq/mg). Beyond this value, m‐IBG uptake mechanisms were saturated. Study of release kinetics showed a rapid first phase (50% released after 4 hr) and a slower second phase (30% of the value retained at the equilibrium point was present after 48 hr), indicating the existence of a storage compartment. Autoradiography studies confirmed the intracytoplasmic localization of m‐IBG and showed that a low percentage (3 to 5%) of SK‐N‐SH cells strongly retained m‐IBG. Cytotoxicity tests showed that SK‐N‐SH cell growth was significantly reduced during the first days of culture, following 2 hr incubation with 1,500 KBq of 125I‐m‐IBG, whereas no toxic effect on SK‐N‐MC cells was found at the same activity. Moreover, the toxic effect observed in the SK‐N‐SH line was clearly related to the use of 125I‐m‐IBG since the same activity of 1,500 KBq of noncoupled 125I was without effect. For the latter line, colonyforming capacity was reduced for activities of 150 and 1,500 KBq of 125I‐m‐IBG, with respectively 32% and 38% lower survival rates. The cytotoxic effect of labelled m‐IBG was, however, limited in non‐saturating concentrations because the specific activity used was too low. Moreover, the low number of cells reconcentrating m‐IBG is indicative of the heterogeneous cellular composition of the SK‐N‐SH line.
Effect of gamma interferon on HLA class‐I and ‐II transcription and protein expression in human breast adenocarcinoma cell lines
Authors: Jabrane‐Ferrat Nabila , Faille Annick , Loiseau Pascale , Poirier Odette , Charron Dominique , Calvo Fabien 
Published: February 2007

AbstractThe spontaneous expression of HLA class‐I and class‐II molecules in 5 human breast carcinoma cell lines, MCF‐7, T47D, ZR75‐I, HSL‐53, MDA‐MB 231, and their modulation during IFN‐γ treatment, are reported. The expression of cell‐surface determinants was examined by indirect immunofluorescence using monoclonal antibodies (MAbs) specific for HLA class‐I and class‐II (DR, DQ and DP) antigens. The biosynthesis and maturation of these molecules were analyzed by 2‐dimensional gel electrophoresis analysis (2D‐PAGE) of class I, DRα, β and invariant immunoprecipitates. Transcription was analyzed by Northern blot hybridization with HLA class‐I and ‐II cDNA‐specific probes. In all cell lines, more than 80% of cells expressed HLA class‐I antigens at their surface. 2D‐PAGE and mRNA studies showed a variable basal level of HLA class‐I biosynthesis and transcription with a constant increase after 1,000 U/ml IFN‐γ treatment. HLA class‐II determinants were totally absent from the surface of MCF‐7, MDA MB231, ZR75‐I and T47D but they were detected in a small subpopulation of HSL‐53 cells (DR 6%, DQ 6%, DP 20%). Spontaneous biosynthesis of HLA‐DR molecules in immunoprecipitates analyzed by 2D‐PAGE or transcripts in Northern blot were not detected in the 5 cell lines. Treatment with 1000 U/ml IFN‐γ Induced or increased the expression of HLA class‐II molecules in all cell lines but DQ expression was variable. While T47D, ZR75‐I and HSL‐53 increased their transcripts and antigen expression, MDA, MB231 and MCF‐7 showed no DQ mRNA transcript. Biochemical analysis of the DR products revealed a classical α, β and invariant (Ii) chain pattern, but indicated a constant glycosylation defect in the invariant chain in all cell lines, associated with weak expression of the β chain and the presence of an extra spot of low molecular weight in the acidic part of the gel. Thus, post‐transcriptional events did not appear to be totally controlled by IFN‐γ in the different cell lines. These differences in DQ expression and glycosylation process in different breast cancer cells may be important in the activation of the immune response among different individuals.
The sialosyl Lewisa ganglioside is present in tumorigenic human urothelial cell lines
Authors: Ugorski Maciej , Påhlsson Peter , Dus Danuta , Nilsson Bo , Skouv Jan , Radzikowski Czeslaw 
Published: February 2007

AbstractThe sialosyl Lewisa antigen was identified in a ganglioside extract from a malignant human urothelial cell line (Hu 1703He) by fast atom bombardment mass spectrometry. The presence of this antigen in urothelial cell lines with varying tumorigenic properties was further studied using the 19‐9 monoclonal antibody (MAb). The sialosyl Lewisa ganglioside was expressed only in the tumorigenic cell lines. Thus, the expression of this antigen is a marker of malignancy for human urothelial cell lines. Mass spectrometry also suggests that the fucosyl GM1 ganglioside was expressed in the Hu 1703He cell line. This was confirmed using the F12 MAb, specific for fucosyl GM1. However, the expression of this antigen was not confined to cell lines with tumorigenic properties.
Melanoma risk by age and socio‐economic status
Authors: Kerkpatrick Constance S. , Lee John A. H. , White Emily 
Published: February 2007

AbstractTo explore the relationship between melanoma incidence, socio‐economic status (SES), and site of occurrence, 1,804 cases of melanoma in Washington State from 1974‐85 were analyzed by mean income of census tract of residence. (Census tracts are geographic divisions of state counties for which demographic information is provided by state agencies.) For males aged 30‐69, as expected, the risk increased with in come. Unexpectedly, at age 70 and over the trend was reversed (the heterogeneity of the age relationship was unlikely to be due to chance). In the older men, the increase with low income was found in the cases reported as Hutchinson's melanotic freckle and those which were not, being statistically significant in the latter. These findings suggest a role for long continued sun exposure in the etiology of malignant melanoma.
Aromatic amine acetyltransferase as a marker for colorectal cancer: Environmental and demographic associations
Authors: Wohlleb James C. , Hunter Carolyn F. , Blass Baxter , Kadlubar Fred F. , Chu David Z. J. , Lang Nicholas P. 
Published: February 2007

AbstractIn this pilot, case‐controlled investigation of 43 colorectal and 41 control male patients, we compared associations of colorectal cancer with the aromatic amine acetyltransferase polymorphism, nutritional and demographic characteristics, medical histories, industrial and occupational histories, and exposures from home environments and personal habits. Persons with the “fast” acetylator trait were at greater risk of colorectal cancer (odds ratio: 2.48; 95% confidence interval: 1.02, 6.03). Results that differed from previous reports were positive associations of colorectal cancer with agricultural and manufacturing industries and with consumption of meats prepared by smoking, curing, and barbecueing. As expected, exercise frequency, cruciferous vegetables, and dietary fiber served as protective factors.
The tumour spectrum in hereditary non‐polyposis colorectal cancer: A study of 24 kindreds in the netherlands
Authors: Vasen Hans F. A. , Johan G. , Offerhaus A. , Den Hartog Jager Frieda C. A. , Menko Fred H. , Nagengast Fokko M. , Griffioen Gerrit , Van Hogezand Ruud B. , Heintz A. Peter M. 
Published: February 2007

AbstractThe hereditary colonic cancer syndrome without polyposis, hereditary non‐polyposis colorectal cancer (HNPCC), is usually divided into 2 main categories: hereditary site‐specific colorectal cancer (Lynch syndrome I) and colorectal cancer in association with other forms of cancer (Lynch syndrome II). One problem associated with Lynch II is the uncertainty as to which types of cancer form part of the hereditary tumour spectrum. The present study was performed to obtain more information about the tumour spectrum of HNPCC. In the 24 HNPCC families studied, 104 patients had colorectal cancer (mean age at diagnosis: 46 years) and in 4 of the families this was the only type of cancer to occur. Sixty‐five extra‐colonic tumours were diagnosed in 20 families. Endometrial carcinoma was found in 16 patients belonging to 12 families. Cancer of the stomach occurred in 10 patients representing 5 families, and mainly in the older generations. Urinary‐tract tumours were found in 8 patients from 4 families. Second primary tumours were diagnosed in 13 of the 16 patients with endometrial cancer, in 4 of the 10 patients with stomach cancer and in 7 of the 8 patients with a urinary‐tract tumour. Many other types of carcinoma were found as well, but less frequently. In our families, the trait appears to be transmitted by patients with cancer of the stomach, endometrium or urinary tract, because some of their children have developed colorectal cancer. The findings suggest that, in these 24 HNPCC families, carcinomas of the endometrium, stomach and urinary tract belong to the hereditary tumour spectrum. Definite assignment of tumours to this spectrum will become possible only after a sensitive and specific biomarker becomes available. The screening programme should depend on which and how many extra‐colonic tumours occur in a family.
Generation and characterization of monoclonal antibodies to prostate secretory protein
Authors: Wright George L. , Huang Chia‐Ling , Lipford Grayson , Beckett Mary Lou , Liang Hong Mei , Haley Cara , Newhall Kathy , Morningstar Michelle 
Published: February 2007

AbstractMonoclonal antibodies (MAbs) were produced against a highly purified preparation of prostate secretory protein (PSP) isolated from normal seminal plasma. Fifteen antibodies were selected for further evaluation based on their strong reactivity and specificity for PSP. All the MAbs had a specificity for prostate epithelial cells and none reacted to any of a variety of normal tissues as determined by immunoperoxidase staining. Six of the MAbs were selected for further immunohistochemical evaluation based on their ability to recognize different antigenic determinants. Using competitive binding immunoassays, a variety of overlapping specificities were observed with at least 2 distinct epitopes identified. Although some staining variability was noted, the 6 antibodies, in general, gave the same pattern of tissue reactivity. Both the normal prostate and the benign prostate hyperplastic ductal epithelial cells stained intensely, with 78 to 100% and 50‐100% of the cells staining, respectively. The number and often the staining intensity of the tumor cells decreased as the tumor became more undifferentiated. Approximately 40 to 100% and 15 to 70% of the tumor cells stained in the moderately‐differentiated and well‐differentiated carcinoma tissues, respectively, whereas either no staining was observed or less than 20% of the tumor cells stained in the poorlydifferentiated and undifferentiated tumors. Most of the metastatic prostate tumors showed either no staining or scattered stalning in a few cells (i. e., less than 20%).
Identification of a 43‐kda nuclear antigen associated with proliferation by monoclonal antibody k 112
Authors: Quak Jasper J. , Dongen Guus Van , Koken Marie A. E. , Brakkee Joost G. P. , Scheper Rik J. , Balm Alfons J. M. , Jong Luitzen De , Snow Gordon B. , Meijer Chris J. L. M. 
Published: February 2007

AbstractMonoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous‐cell carcinoma. The antibody detects a 43‐kDa nuclear antigen, with a pl of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell‐cycle‐related nuclear antigen molecules.
Genotype markers and proto‐oncogene analysis in the cd30‐positive “malignant histiocytosis” del cell line with t(5;6)(q35;p21)
Authors: Gogusev J. , Barbey S. , Nezelof C. 
Published: February 2007

AbstractThe DEL cell line isolated from a patient who died of malignant histiocytosis exhibits a reciprocal chromosomal translocation t(5;6) (5q35;6p21). The cells were analyzed for lg(Jh), TCR β‐gene rearrangements and proto‐oncogene expression pattern, using a panel of molecularly cloned probes that in cluded c‐fms, c‐myc, c‐myb, c‐pim, c‐fos, N‐myc, c‐sis, c‐fgr as well as the virally derived probes v‐ki‐ras and v‐src. Consistent levels of expression of c‐fms, c‐myc, c‐myb, c‐ki‐ras and c‐fgr were identified in cells from several in vitro passages as well as from the heterotransplanted tumors in nude mice. Transcripts homologous to the c‐fos, c‐src and c‐sis were not observed. Southern blot study of DNA showed that the banding pattern of the screened proto‐oncogenes was not altered. Furthermore, Southern blot analysis demonstrated monoallelic immunoglobulin heavy chain (IgJh) rearrangement but a normal germ‐line configuration of the K light chain and TCR β‐genes. These results appear to imply that a T‐ or B‐cell origin can be eliminated and that several activated protooncogenes, usually expressed in immature MPS cells (c‐fms) and myeloblastic cells (c‐fgr), may be implicated in the proliferative activity of the DEL cell line, the stem of which may be a primitive, ancestral myelomonocytic cell.
Human multi‐drug‐resistant cancer cells exhibit a high degree of selectivity for stereoisomers of verapamil and quinidine
Authors: Eliason James F. , Ramuz Henri , Kaufmann Franz 
Published: February 2007

AbstractAn in vitro cell proliferation assay was developed to measure the capacity of substances to overcome multi‐drug resistance (MDR). The assay is a modification of the MTT (3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) method. The inclusion of cell titration curves for each concentration of the resistance modifier (RM) allows the IC50 of the RM to be calculated and provides empirical correction of the cell survival curves for the effect of the RM when it is combined with a standard cytotoxic drug, vincristine. The resistance modification index (RMI) is defined as the ratio of the IC50 of vincristine obtained in control cultures divided by that measured in the presence of RM and is linearly related to the dose of RM. The RMI0.1, the RMI at a one‐tenth the IC50 of the RM, provides a relative comparison between the activities of different RMs at non‐toxic doses. The results obtained using the MDR cell line, KB‐8‐5, show that I‐(‐)‐verapamil is approximately 4 times more active than d‐(+)‐verapamil in modifying MDR. The racemic mixture has an intermediate activity. A similar comparison between the epimers quinidine and quinine shows that, at equimolar doses, quinine has a higher RMI but, because it is more toxic, the RMI0.1 is about one‐half of that of quinidine. These results demonstrate the importance of comparing the resistance‐modifying activities of different compounds at doses relative to their own toxicity.
Newly established human retinoblastoma cell lines exhibit an “immortalized” but not an invasive phenotype in vitro
Authors: Griegel Sahine , Hong Chen , Frötschl Roland , Hülser Dieter F. , Greger Valerie , Horsthemke Bemhard , Rajewsky Manfred F. 
Published: February 2007

AbstractRetinoblastoma (RB), an intraocular childhood tumor occurring in a hereditary (mostly bilateral) or non‐hereditary (unilateral) form, is associated with the inactivation of both alleles of a putative tumor suppressor gene (RB‐I) located on chromosome 13q14. Both the process of RB development and the biological characteristics of RB cells are as yet poorly understood. We have established 7 new RBL lines (RBL13, RBL14, RBL18 and RBL30, derived from unilateral RB; and RBL7, RBL15 and RBL20, derived from bilateral RB). Southern blot analyses of restriction fragment length polymorphisms in DNA samples from 6 cell lines revealed loss of constitutional heterozygosity at one or several polymorphic locion chromosome 13 in 4 cases. Gross deletions involving the RB‐1 locus and amplification of the N‐myc gene were not detected in any of the RBL lines. The phenotypic properties of the RBL lines were analyzed in comparison with cells from the original RB tumors, with 4 RB lines established by others (RB383, RB355, RB247C3 and Y79) and with the adenovirus‐EIA‐transformed human retinoblast line HER‐Xhol ‐CC2. It was found that RB tumors consist of phenotypically heterogeneous cell subpopulations with varying nutrient requirements and differentiation potential in vitro. All cell lines showed the typical characteristics of established (immortalized) cells. In some cases, cells from original RB tumors or cell lines were able to form colonies when cell aggregates of 2‐10 cells were suspended in semi‐solid agar medium; however, anchorage‐independent colonies never developed from single cells. Cell lines RBL13, RBL18, RB247C3, RB355, RB383 and Y79 were tested for invasion into embryonic chick heart fragments in vitro and found to be non‐invasive. None of the RBL or RB lines were tumorigenic in nu/nu (T‐) mice. Y79 cells (propagated in culture for many years) exhibited properties distinctly different from those of the other cell lines, and thus cannot be considered phenotypically representative of RB cells.
A study of human small‐cell lung carcinoma (hsclc) cell lines with different sensitivities to detect relevant mechanisms of cisplatin (cddp) resistance
Authors: Hospers G. A. P. , Meuer C. , De Leij L. , Uges D. R. A. , Mulder N. H. , De Vries E. G. E. 
Published: February 2007

AbstractThe cisplatin(CDDP)‐resistant cell line GLC4‐CDDP shows a variety of differences from the parent line GLC4. The aim of this study was to determine which of the observed changes correlated with the degree of resistance and was therefore relevant to the phenomenon of CDDP resistance. For these experiments we used cells of the sensitive hSCLC cell line GLC4 and the in vitro‐acquired CDDP‐resistant sublines GLC4‐CDDP3 and GLC4‐CDDP11, with a resistance factor (RF) of 3 and 11 respectively for CDDP and of 1.8 and 7.4 respectively for carboplatin. Carboplatin was used, in addition to CDDP in seeking relevant mechanisms. No consistency was found between the RF and the growth pattern or antigen expression, cellular volume, doubling time, cellular or nuclear platinum (Pt) content or the level of Pt‐non‐histone chromatin protein (NHCP) binding. A correlation was found between the RF and the level of glutathione (GSH), and a trend was found for the level of Pt‐DNA binding, Pt‐GG adduct content and the amount of interstrand cross‐links (ISC). These changes might therefore be relevant for the development of resistance. These findings are compatible with a GSH‐induced reduction of the amount of reactive Pt in the resistant cell, resulting in a lower net platination and toxic Pt‐DNA adduct formation.
Serum changes the response of cultured morris hepatoma 7777 cells to autocrine growth factors
Authors: Guzik Krzysztof , Klein Andrzej 
Published: February 2007

AbstractThe addition of calf serum to culture medium alters the biological response of Morris hepatoma 7777 (MH) cells to autocrine growth factors isolated from conditioned medium of the investigated cells. Acetic acid (AA) extract obtained from conditioned medium of MH cells showed a change in anchorage‐independent growth‐regulatory activity from stimulation (serum‐free) to inhibition (10% calf serum). Two protein fractions of apparent molecular weight 15 and 7.5 kDa isolated from AA‐extract by Bio‐Gel P‐60 filtration also changed their growth‐regulatory activity after supplementation of culture medium with calf serum. The contradictory effect of autocrine regulators estimated in soft agar using the 3H‐thymidine (3H‐TdR) incorporation test and colony formation assay gave generally comparable results, except in the case of the 15‐kDa fraction. The most active 7.5‐kDa fraction stimulated 3H‐TdR incorporation and colony formation in serum‐free medium up to about 300 and 500% respectively, while in the presence of 10% calf serum inhibition of about 20 and 50% has been observed. Our results suggest that the fraction contains an autocrine growth factor(s), whose activity is inverted in the presence of serum.
Natural history of colorectal polyps and the effect of polypectomy on occurrence of subsequent cancer
Authors: Murakami Ryosuke , Tsukuma Hideaki , Kanamori Shinobu , Imanishi Kiyoshi , Otani Toru , Nakanishi Katsumi , Fujimoto Isaburo , Oshima Akira 
Published: February 2007

AbstractTo elucidate the natural history of colorectal polyps and to observe the influence of endoscopic polypectomy on the incidence of colorectal cancer, we conducted a retrospective cohort study of all patients who had undergone colonoscopic examination at the Center for Adult Diseases, Osaka, between April 1974 and December 1985. The study subjects consisted of a control group (760 non‐polyp patients) and a polyp group (648 polyp patients, including 136 treated by polypectomy at the initial examination). These subjects were followed up until the end of 1987 by record linkage with the Osaka Cancer Registry's file to observe the occurrence of colorectal cancer. The O/E (observed/expected numbers derived from the general population) was 5.1 (95% confidence interval = 2.5‐9.4) and 1.0 (0.1‐3.6) for the polyp and control group, respectively. When subjects in the polyp group were categorized into polypectomy and non‐polypectomy sub‐groups, the O/E was 2.3 (0.1‐12.6) and 8.0 (3.4‐15.8) respectively. The relative risk of undergoing polypectomy to developing subsequent cancer was estimated at 0.3 (0.1‐2.1). These results suggest an increased risk of developing cancer among polyp patients and the possibility of prophylactic effect of polypectomy against subsequent cancer. A large‐scale and longterm follow‐up study is required to confirm these findings.