bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of osteoarthritic cartilage using monoclonal antibodies reactive with rabbit proteoglycan
Authors: Kresina Thomas F. , Malemud Charles J. , Moskowitz Roland W. 
Published: February 2007

AbstractFemoral and tibial cartilage specimens from nonoperated, sham operated, and partially meniscectomized knees of New Zealand white rabbits were studied, using fluorescein‐conjugated mouse IgG or 3 monoclonal antibodies (2G2, 2E9, and 6C9) that portrayed differing fine antigenic specificity for rabbit cartilage proteoglycan monomer. In nonoperated and sham operated animals, monoclonal antibodies 2G2 and 2E9 stained cellular/pericellular (C/PC) and matrix areas; antibody 6C9 stained only C/PC areas. Augmented C/PC and matrix staining of osteoarthritic femoral cartilage occurred with 2G2 and 6C9; tibial cartilage staining was reduced. Increased 2E9 staining of C/PC regions was seen in tibial cartilage. The differential staining patterns indicate that proteoglycan macromolecular changes occur in experimental osteoarthritis and that these monoclonal antibodies can be utilized as probes for the connective tissue changes observed.
Correlation between the presence of antibodies to the Epstein‐barr virus nuclear antigen type 2 and antibodies to the rheumatoid arthritis nuclear antigen in patients with rheumatoid arthritis
Authors: Sculley Thomas B. , Pope John H. , Hazelton Ron A. 
Published: February 2007

AbstractThe incidence of antibodies to Epstein‐Barr nuclear antigen type 2 (EBNA‐2) was determined in sera from rheumatoid arthritis (RA) patients and control subjects, by protein immunoblotting. Sixty‐eight percent of the RA patients and 48% of the controls possessed anti‐EBNA‐2 antibodies. The titer of anti‐rheumatoid arthritis nuclear antigen (RANA) in RA patient sera showed a stronger correlation with serum reactions to EBNA‐2 than with reactions to EBNA‐1. Our results indicate that the presence of EBNA‐2 may make a major contribution to the RANA reaction.
Retinopathy in systemic lupus erythematosus: Relationship to disease activity
Authors: Klinkhoff Alice V. , Beattie Craig W. , Chalmers Andrew 
Published: February 2007

AbstractForty‐three patients taking chloroquine for systemic lupus erythematosus were followed by one ophthalmologist over a 5‐year period. Visual field testing, color vision testing, and fluorescein angiography were performed. Retinopathy was detected in 7 patients (16%), none of whom had hypertension or diabetes mellitus. Retinal abnormalities included cotton‐wool spots in 4 patients, microaneurysms in 3, and vascular tortuosity in 4. In 4 patients, these abnormalities were associated with retinal dysfunction, measured in terms of abnormal hue discrimination. In 6 of the 7 patients, the finding of retinopathy coincided with a flare of lupus activity. In 5 patients, retinopathy improved when the disease was controlled.
The early diagnosis of ischemic necrosis of bone
Authors: Zizic T. M. , Marcoux C. , Hungerford D. S. , Stevens M. B. 
Published: February 2007

AbstractOne hundred sixty‐nine patients with radio‐graphic or histologic evidence of ischemic necrosis of bone (INB) were evaluated. Ninety‐nine (59%) of the 169 patients had multiple sites of INB, with 310 bones affected. Two hundred sixty‐three (85%) of the 310 ischemic bones were symptomatic. Routine radiography produced negative findings in 58 (20%) of the bones with histologically confirmed INB. Results of hemodynamic studies, including baseline bone marrow pressure, saline stress test, and/or intraosseous venography, were abnormal in 243 (94%) of 259 ischemic bones so evaluated. Most importantly, hemodynamic studies detected 51 (93%) of the 55 bones that were radiographically normal but had histologically confirmed INB.
Elevated MMPI scores for hypochondriasis, depression, and hysteria in patients with rheumatoid arthritis reflect disease rather than psychological status
Authors: Pincus Theodore , Callahan Leigh F. , Bradley Laurence A. , Vaughn William K. , Wolfe Frederick 
Published: February 2007

AbstractThe Minnesota Multiphasic Personality Inventory (MMPI) scales for Hypochondriasis, Depression, and Hysteria were studied in patients with rheumatoid arthritis (RA). The RA patients showed elevated scores on these scales, and these results are similar to those reported in each of 6 published studies. The elevated MMPI scale scores can be explained largely by 5 “disease‐related” MMPI statements which met 2 criteria: (a) they were among 11 of the 117 MMPI statements that two‐thirds of rheumatologists predicted would be RA‐associated; and (b) RA patients and normal subjects differed significantly in their responses to these statements. The responses of RA patients and normal subjects to most other statements in the MMPI Hypochondriasis, Depression, and Hysteria scales were quite similar. In RA patients, responses to “disease‐related” statements were correlated with results of measures of disease activity, which indicates that responses to these MMPI items reflect the severity, as well as the presence, of RA. These findings suggest that new criteria are needed for validation of the MMPI as a clinical tool for the recognition of hypochondriasis, depression, and hysteria in a patient who has RA.
Inorganic pyrophosphate release by rabbit articular chondrocytes in vitro
Authors: Prins A. Pieter A. , Kiljan Erno , van de Stadt Rob J. , van der Korst Jan K. 
Published: February 2007

AbstractRelease of inorganic pyrophosphate (PPi) by rabbit articular chondrocytes in vitro was measured by a newly developed assay which utilizes radioactive orthophosphate (32Pi) labeling and anion exchange high performance liquid chromatography. Chondrocytes in monolayer and high density culture failed to release PPi. Explants (cartilage fragments), however, released newly formed PPi into the culture medium. Trypsin treatment of cartilage fragments almost completely blocked the PPi extrusion. Collagenase treatment had no effect on PPi extrusion. There was no clear correlation between proteoglycan synthesis, measured by 35SO4 incorporation, and PPi release. Suppression of proteoglycan synthesis with tunicamycin did not influence the PPi release of the explants.
Protein traffic in human synovial effusions
Authors: Wallis Wayne J. , Simkin Peter A. , Nelp Wil B. 
Published: February 2007

AbstractConcentrations of 5 marker proteins were measured in synovial fluid and serum samples from knee effusions of 11 patients with rheumatoid arthritis and 9 with osteoarthritis. Indirect determinations of synovial plasma flow and lymphatic drainage were obtained by measuring iodide clearance (ml/minute) and radio‐albumin clearance (ml/minute). Together with protein concentrations, these determinations allowed us to calculate: 1) the flux of each marker protein through synovial tissues (mg/minute); 2) the volume of plasma cleared by synovium per unit time (ml/minute); and 3) the fractional extraction per passage through the synovial microcirculation (protein permeance). These measures differed substantially between rheumatoid arthritis and osteoarthritis patient populations and quantified the severity of the microvascular lesion in rheumatoid synovitis.
Inclusion body myositis presenting as treatment‐resistant polymyositis
Authors: Calabrese Leonard H. , Mitsumoto Hiroshi , Chou Samuel M. 
Published: February 2007

AbstractInclusion body myositis (IBM) has been viewed as a distinct and rare form of inflammatory myopathy. Previously reported findings from series of IBM patients have suggested that clinical and pathologic features are present which readily distinguish it from idiopathic polymyositis. We report 4 cases of IBM presenting clinically and pathologically as polymyositis, each of which was refractory to therapy. Our data suggest that IBM may be a more common and heterogeneous form of inflammatory myopathy than has been previously suggested. Furthermore, IBM may be clinically and electrophysiologically indistinguishable from polymyositis. Reasons for failing to recognize IBM by pathologic studies appear to include: the skip lesion nature of the pathologic findings, failure to examine tissues by electron microscopy, and a low level of suspicion or lack of recognition. Because of its insidious clinical course and its failure to respond to immunosuppressive therapy, IBM may be an important variant of treatmentresistant polymyositis.
An antiserum to a disease‐associated factor from the cells of an hla–B27 positive patient with ankylosing spondylitis specifically recognizes an hla–B27 associated determinant
Authors: Sullivan J. S. , Geczy A. F. 
Published: February 2007

AbstractAntiserum raised to a factor elaborated by a lymphoblastoid cell line derived from the peripheral blood cells of an HLA–B27 positive patient with ankylosing spondylitis specifically lyses the B27 positive, but not the B27 negative, cells of ankylosing spondylitis patients. The cells of B27 positive and B27 negative normal controls are not lysed. This serum has similar specificity to antisera against cross‐reactive bacteria.
Anticardiolipin antibodies in patients with rheumatic diseases
Authors: Fort John G. , Cowchock F. Susan , Abruzzo John L. , Smith J. Bruce 
Published: February 2007

AbstractRecent attention has focused on the presence of anticardiolipin (ACL) antibodies and their possible role in recurrent thrombosis and abortions in patients with systemic lupus erythematosus. We analyzed ACL antibodies in 243 consecutive patients to determine their frequency in patients with different rheumatic disorders. A significantly elevated frequency was found in patients with systemic lupus erythematosus (38%), rheumatoid arthritis (33%), and psoriatic arthritis (28%). No correlation could be found between ACL antibody levels and recurrent thrombosis. In patients with rheumatoid arthritis there was a significant correlation between ACL antibodies and a history of repeated abortions. No significant association was found between ACL antibodies and other autoantibodies except in patients with rheumatoid arthritis; ACL antibody‐positive rheumatoid arthritis patients were much more likely to be antinuclear antibody‐positive (P < 0.0002).
Hyaluronate inhibition of cell proliferation
Authors: Goldberg Ronald L. , Toole Bryan P. 
Published: February 2007

AbstractThe proliferation of rabbit synovial cells, 3T3 cells, or simian virus–transformed 3T3 cells in cell culture was inhibited by the addition of hyaluronate to the culture medium. This effect was markedly dependent on the molecular weight and concentration of the hyaluronate. At the molecular weight and concentration of hyaluronate present in normal synovial fluid, proliferation was inhibited. At lower molecular weights or concentrations, as found in rheumatoid synovial fluid, hyaluronate was significantly less inhibitory. Thus, the changes in synovial fluid hyaluronate that are associated with arthropathies may contribute to a favorable environment for rheumatoid pannus expansion.
Frequency of infections among rheumatoid arthritis patients, before and after disease onset
Authors: Vandenbroucke Jan P. , Kaaks Rudolf , Valkenburg Hans A. , Boersma Johannes W. , Cats Arnold , Festen Johan J. M. , Hartman Arend P. , Huber‐Bruning Olga , Rasker Johannes J. , Weber Jacobus 
Published: February 2007

AbstractThe self‐reported frequency of genitourinary and bronchopulmonary infections in postmenopausal women with rheumatoid arthritis (RA) and in postmenopausal women with osteoarthritis and/or soft tissue rheumatism was compared. Neither before, nor after the onset of joint disease was a higher frequency reported by the RA patients. The previously established increased mortality from infectious disease among RA patients might be due to a more severe infectious disease course, leading to an increased case‐fatality rate.
Evidence of immune deposits and of basement membrane alterations in dermal vessels of normal skin of patients with essential mixed cryoglobulinemia
Authors: Gabrielli Armando , Sbarbati Andrea , Marchegiani Gabriele , Rupoli Serena , Montroni Maria , Cinti Saverio , Danieli Giovanni 
Published: February 2007

AbstractTo gain insight into the pathogenic mechanisms of immune complex–mediated vasculitis, clinically normal skin specimens from the forearms of 12 patients with essential mixed cryoglobulinemia were investigated by light microscopy, immunofluorescence microscopy, and electron microscopy. Basement membrane alterations were documented in 9 patients. Eleven patients had deposits in vessel walls, but only in 1 was there evidence of inflammation. The same immunoglobulins of the cryoproteins could be demonstrated in the tissue deposits by immunofluorescence analysis and by immunoelectron microscopy. These findings suggest that, in essential mixed cryoglobulinemia, immune reactants in dermal vessels of normal skin are more common than is evidenced by clinical examination. Moreover, it seems that cryoglobulins, as such, are not sufficient to trigger an inflammatory process: Additional local or plasma factors are required. Deposits were absent in 7 patients with cryoglobulinemia that was associated with a primary disorder. This could be ascribed to the lower cryocrit levels documented in this group of patients, or to the shorter duration of their disease.
Effects of dietary supplementation with marine fish oil on leukocyte lipid mediator generation and function in rheumatoid arthritis
Authors: Sperling Richard I. , Weinblatt Michael , Robin Jean‐Louis , Iii Joseph Ravalese, , Hoover Richard L. , House Frank , Coblyn Jonathan S. , Fraser Patricia A. , Spur Bernd W. , Robinson Dwight R. , Lewis Robert A. , Austen K. Frank 
Published: February 2007

AbstractTwelve patients with active rheumatoid arthritis supplemented their usual diet with 20 gm of Max‐EPA fish oil, daily, for 6 weeks. Following this supplementation, the ratio of arachidonic acid to eicosapentaenoic acid in the patients' neutrophil cellular lipids decreased from 81:1 to 2.7:1, and the mean generation of leukotriene B4 (with calcium ionophore stimulation) significantly declined by 33%. The mean neutrophil chemotaxis to both leukotriene B4 and FMLP significantly increased toward the normal range at week 6. The generation of 5‐lipoxygenase products by calcium ionophore‐stimulated monocytes was not significantly suppressed, but a significant decline (37%) in plateletactivating factor generation was noted at week 6. The modulation of these measures of leukocyte inflammatory potential suggests that fish oil supplementation may have an antiinflammatory effect.
Arthroscopic and immunohistologic characterization of knee joint synovitis in osteoarthritis
Authors: Lindblad Staffan , Hedfors Eva 
Published: February 2007

AbstractWe studied 10 patients who had arthritis of the knee joint, but no other signs of rheumatic disease. The clinical diagnosis of osteoarthritis was corroborated by arthroscopic evidence of characteristic cartilage degeneration. Signs of inflammation were confined to areas of the synovial membrane that lay near the cartilage; thus, the major part of the joint cavity was not affected. The intensity of the synovial inflammation varied within the areas involved, but was always most pronounced in regions rimming the cartilage. Biopsy samples selected from regions of intensely inflamed synovium contained foci of T lymphocytes, which were bordered by immunoglobulin‐carrying B lymphocytes and plasma cells, as well as strongly HLA‐DR positive dendritic‐like cells adjoined to αLeu − 3a + T helper lymphocytes. In tissue samples taken from macroscopically noninflamed areas, only a few infiltrating lymphocytes were seen. Thus, the inflammatory synovial changes found in osteoarthritis appear to be anatomically restricted and of varied intensity but, when present, are microscopically indistinguishable from the changes that have been previously described as indicative of rheumatoid arthritis.
Hla‐dr4 in giant cell arteritis: association with polymyalgia rheumatica syndrome
Authors: Richardson Joan E. , Gladman Dafna D. , Fam Adel , Keystone Edward C. 
Published: February 2007

AbstractForty‐three patients with well‐documented giant cell arteritis (GCA) were studied clinically and with HLA typing. All patients were over age 40. Twenty‐two of the patients had coexistent polymyalgia rheumatica (PMR). No association with class I HLA antigens was detected. When compared with HLA findings in 243 healthy controls, HLA‐DR4 was increased in patients with GCA and PMR, but not in patients with GCA alone. Pooled analysis of data from 4 published papers confirmed the association of DR4 with GCA and PMR (P /0.00001). Patients with PMR but negative findings of temporal artery biopsies also showed an increased frequency of HLA‐DR4, but this did not reach statistical significance, probably because of the small number of subjects. It is likely that the increased frequency of DR4 in GCA patients is related to its association with PMR.
Dissection of the mechanisms of immune injury in rheumatoid arthritis, using total lymphoid irradiation
Authors: Gaston J. S. H. , Strober S. , Solovera J. J. , Gandour D. , Lane N. , Schurman D. , Hoppe R. T. , Chin R. C. , Eugui E. M. , Vaughan J. H. , Allison A. C. 
Published: February 2007

AbstractEleven patients with intractable rheumatoid arthritis were treated with total lymphoid irradiation. After radiotherapy, there was a marked decrease in the number and function of peripheral blood helper/inducer (Leu‐3 +) T lymphocytes, in the spontaneous secretion of interleukin‐1 by synovial biopsy specimens, and in the activity of the joint disease. In contrast, levels of IgM, IgA, and IgG rheumatoid factors and C3 concentrations in blood and synovial fluid samples did not change significantly after therapy with total lymphoid irradiation.
Radiographic and joint count findings of the hand in rheumatoid arthritis. related and unrelated findings
Authors: Fuchs Howard A. , Callahan Leigh F. , Kaye Jeremy J. , Brooks Raye H. , Nance E. Paul , Pincus Theodore 
Published: February 2007

AbstractRadiographic and physical joint count findings of the hands and wrists of 148 patients with rheumatoid arthritis (RA) were analyzed in a cross‐sectional study. Quantitative radiographic scores for joint space narrowing, erosion, and malalignment were correlated highly with joint count scores for limitation of motion and deformity, and were correlated at considerably lower levels with joint swelling scores. Radiographic scores, however, were not correlated at all with joint count tenderness scores. That 2 of the most widely used indices of RA disease activity, radiographic erosion scores and joint count scores for tenderness, were independent of one another at a selected timepoint, should be considered in the design of clinical trials and long‐term observation of patients with RA.
Arthritis in idiopathic hypereosinophilic syndrome
Authors: Manuel Martían‐Santo71s J. , Mulero Juan , Andréau J. Luis , De Villa Luis F. , Quiróas Luis Bernaldo‐De , Noguera Enrique 
Published: February 2007

AbstractA 54‐year‐old man was admitted for tenosynovitis of the wrists, olecranon bursitis, stiffness, progressive flexion of the fingers, subcutaneous nodules, and persistent blood eosinophilia. A high eosinophil count was detected in his synovial fluid. We review the literature on idiopathic hypereosinophilic syndrome. This case provides evidence that nonerosive polyarthritis with periarticular involvement and nodules can be manifestations of this syndrome.
The ai/rheum knowledge‐based computer consultant system in rheumatology. performance in the diagnosis of 59 connective tissue disease patients from japan
Authors: Porter James F. , Kingsland Iii Lawrence C. , , Lindberg Donald A. B. , Shah Indravadan , Benge James M. , Hazelwood Susan E. , Kay Donald R. , Homma Mitsuo , Akizuki Masashi , Takano Makoto , Sharp Gordon C. 
Published: February 2007

AbstractAI/RHEUM is a knowledge‐based computer consultant system for the diagnosis of rheumatic diseases. Its diagnostic accuracy was evaluated using information that was supplied by Japanese rheumatologists on 59 patients with connective tissue diseases. The diagnoses of the AI/RHEUM model were in full or partial agreement with those of the Japanese rheumatologists in 54 of 59 cases (92%). Preliminary evaluation of the criteria used by the model to diagnose mixed connective tissue disease showed a sensitivity of 90% and a specificity of 96%.
Abnormal glycosylation of serum igg from patients with chronic inflammatory diseases
Authors: Tomana Milan , Schrohenloher Ralph E. , Koopman William J. , Alarcän Graciela S. , Paul William A. 
Published: February 2007

AbstractResults of carbohydrate analysis of serum IgG from patients with rheumatoid arthritis (RA) confirmed an earlier report that IgG from patients with RA is galactosylated to a lesser extent than IgG from healthy individuals. In contrast to the previous report, we found that the content of galactose in IgG from controls and RA patients was negatively correlated with age (P = 0.026 and P = 0.010, respectively). In RA patients, the IgG content of galactose was also negatively correlated with the pain index (P < 0.05) and was lower in the presence of rheumatoid factor (P < 0.05). No correlation was found between the galactose deficiency of IgG from RA patients and sex, race, duration of disease, packed red blood cell volume, radiographic grade, disability index, extraarticular manifestations, articular erosions, or treatment with steroids. Furthermore, no correlation was found between the galactose content of IgG and serum levels of IgM rheumatoid factor or the ability of IgG to bind IgM rheumatoid factor in vitro. Significant galactose deficiency was also detected in IgG from patients with systemic lupus erythematosus and Crohn's disease, which suggests that the defect in the galactosylation of IgG is a feature common to a variety of chronic inflammatory diseases. The biologic significance of this observation remains unclear.
Evidence for the presence of autoantibodies to the collagen‐like portion of c1q in systemic lupus erythematosus
Authors: Antes Ursula , Heinz Hans‐Peter , Loos Michael 
Published: February 2007

AbstractWe investigated the connection between the C1q solid‐phase binding assay (C1q SPBA) and doublestranded DNA antibodies, and analyzed the immune complex material in systemic lupus erythematosus (SLE) sera. Comparison with a new monoclonal assay for C1q‐bearing immune complexes (the 242G3 assay) revealed that the immune complexes in SLE bind specifically to solid‐phase C1q, and not to fluid‐phase C1q. The C1q solid‐phase binding activity sedimented as 7S IgG, was insensitive to DNase treatment, and could be selectively absorbed by C1q‐coupled beads and by bovine serum albumin–anti–bovine serum albumin C1q beads, but not by DNA. Thus, antibodies to doublestranded DNA do not interfere in the C1q SPBA. Isolated IgG from SLE serum precipitated the collagenlike portions, and not the globular, Fc‐recognizing portions, of C1q. F(ab')2 fragments of IgG from SLE patient serum were able to bind C1q. These data show that in SLE sera, especially in those with low levels of CH50 and C1q, autoantibodies that react with the collagen‐like part of C1q are detectable. Since in the C1q SPBA, the C1q molecule is randomly fixed to the solid phase, we can detect not only immune complexes, but also antibodies that react with the collagen part of C1q; this may explain the high percentage of positive results for SLE sera in the C1q SPBA, in contrast to results of other immune complex assays.