bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beta-blocker administration within 24 hours after admission to the intensive care unit and mortality in critical heart failure patients: a retrospective analysis from the MIMIC-IV database
Authors: Xie Linfeng , Chen Jing , Li Yuanzhu , Liu Gang , Shen Jian , Li Xiang , Yang Yuan , Chen Yintao , Luo Suxin , Huang Bi 
Published: February 2025

BackgroundIt remains poorly understood whether early use of beta-blockers could provide a survival advantage in patients with critical heart failure (HF) .MethodsThis retrospective study was conducted using the American Medical Information Mart for Intensive Care (MIMIC)-IV database. Study participants were critical HF patients who were divided into two groups: within 24-hour use of beta-blockers group and no use of beta-blockers group. The primary study endpoints were 7-day, 30-day, and 360-day all-cause mortality.ResultsOut of the 10,184 patients diagnosed with critical HF, after propensity score match (PSM), 7352 patients were recruited and were divided into within 24-h use of beta-blockers group (n = 3676) and no beta blockers group (n = 3676). The 7-day, 30-day, and 360-day all-cause mortality were significantly higher in the no beta blockers group (7-day: 10.3% vs 5.5%; 30-day: 21.4% vs 15.7%; 360-day: 40.0% vs 35.3%; all p &lt; 0.001). Kaplan–Meier analyses showed that the cumulative incidence of 7-day, 30-day, and 360-day all-cause mortality were significantly higher in the no beta blockers group (all log-rank p &lt; 0.001). After PSM, Cox proportional hazards analyses revealed that beta blockers administration within 24 h of admission to intensive care unit (ICU) was independently associated with decreased 7-day (HR = 0.52 95%CI: 0.44, 0.62, p &lt; 0.001), 30-day (HR = 0.70 95%CI: 0.63, 0.78, p &lt; 0.001), and 360-day (HR = 0.83 95%CI: 0.77, 0.89, p &lt; 0.001) all-cause mortality.ConclusionAdministration of beta blockers within 24 h after admission to ICU was associated with reduced risk of mortality in critical HF patients. However, prospective randomized controlled trials are needed to confirm our findings due to the retrospective nature of the present study and the limitations of the MIMIC-IV database itself.
A combinatorial screening protocol for identifying novel and highly potent dual-target inhibitor of BRD4 and STAT3 for kidney cancer therapy
Authors: Zhang Shizhu , Wu Nan , Geng Yifei , Guan Lixia , Niu Miao-Miao , Li Jindong , Zhu Lusha 
Published: February 2025

Concurrent inhibition of bromodomain-containing protein 4 (BRD4) and signal transductor and activator of transcription 3 (STAT3) could potentially be an effective strategy against renal cell carcinoma (RCC). Here, we successfully identified five dual-targeted BRD4/STAT3 inhibitors (BSTs 1–5) using a combinatorial screening protocol. Particularly, BST-4 was the most potent inhibitor simultaneously targeting BRD4 (IC50 = 2.45 ± 0.11 nM) and STAT3 (IC50 = 8.07 ± 0.51 nM). MD simulation indicated that BST-4 stably bound to the active sites of BRD4 and STAT3. The cytotoxicity assays exhibited that BST-4 had a significant antiproliferative activity against RCC cell lines, especially CAKI-2 cells (IC50 = 0.76 ± 0.05 μM). Moreover, in vivo experiments revealed that BST-4 more effectively inhibited the growth of xenograft tumors compared with positive controls RVX-208 and CJ-1383. Overall, these data indicated that BST-4 could be a promising candidate compound for RCC therapy.
When helping hurts: validating a measure of compulsive helping and exploring potential correlates
Authors: Workman Katey , Padilla-Walker Laura M. , Reschke Peter J. , Rogers Adam A. 
Published: February 2025

IntroductionThis cross-sectional study proposes and validates a new measure of compulsive helping: helping which harms the helper.MethodsEmerging Adults (N = 438; Mage = 20.29, SD = 1.04, 51.71% Female) reported on compulsive helping. Confirmatory factor analysis was used to assess construct validity, while bivariate correlations were used to assess convergent and discriminant validity.ResultsConfirmatory factor analysis suggested convergent validity with all 10 items loading onto a single factor, with factor loadings above 0.44. Model fit was acceptable. Convergent validity was demonstrated such that compulsive helping was positively correlated with prosocial behavior and anxiety. Discriminant validity was demonstrated such that compulsive helping was negatively associated with self-regulation.ConclusionThis new concept and measure of compulsive helping is a first step toward defining the limits of the adaptiveness of prosocial behavior. Though not frequent, it appears helping which is harmful is not uncommon. Future research should employ qualitative means and consider the multidimensionality of prosocial behavior.
Study on the impact of rural public sports facilities and instructors on residents’ participation in sports activities in China
Authors: Guo Xiujin , Yang Xiangjun , Mao Sujie 
Published: February 2025

ObjectiveThis study aims to explore how rural public sports facilities and their instructors influence the participation of rural residents in sports activities under the background of China’s rural revitalization strategy. The goal is to provide strategies for the effective use and management of rural sports facilities, thereby encouraging rural residents to actively participate in sports activities, improve their quality of life, and support comprehensive rural revitalization.MethodsA cross-sectional study design was used, employing a stratified sampling method to distribute questionnaires to 5,000 residents in the eastern, central, western, and southern regions of China. Data analysis was conducted using R4.1.3 software. The main research indicators included the funding sources of sports facilities and the composition of social sports instructors, while residents’ basic characteristics were considered secondary indicators for correlation, regression, and cross-analysis.ResultsAnalysis of 3,956 valid questionnaires revealed that increasing age led to a decrease in the frequency of sports activities (−0.098), while higher education levels increased activity frequency (0.097). Facilities provided by county sports bureaus significantly promoted participation in sports activities (B = 0.320, p &lt; 0.001). Facilities donated by villagers or township enterprises and the sports lottery fund also effectively increased participation (B = 0.219, p &lt; 0.001; B = 0.159, p = 0.011). Sports facility instructors, particularly urban residents and retirees, significantly positively impacted activity time, while the absence of instructors reduced residents’ participation in sports activities (B = −0.445, p &lt; 0.001).ConclusionAge and education level are negatively correlated with sports activity participation, while the source of public sports facilities, especially those provided by county sports bureaus, the sports lottery fund, and village committees, significantly enhance participation. The presence of social sports instructors significantly increases the time and frequency of residents’ sports activities. Policymakers need to focus on the construction and management of public sports facilities, develop and utilize diversified funding sources, and enhance the role of sports facility instructors. By providing professional guidance and organizing diverse sports activities, rural residents’ enthusiasm for participation can be effectively stimulated.
Parental smoking is associated with adolescent loneliness: evidence from 71 low- and middle-income countries
Authors: Xie Jintang , Yang Hui , Zhao Min , Magnussen Costan G. , Xi Bo 
Published: February 2025

IntroductionThe association between parental smoking and adolescent mental health is not well understood. We aimed to examine the association between parental smoking and adolescent loneliness using global data collected from the Global School-based Student Health Survey (GSHS) between 2009 and 2019.MethodsA total of 187,552 adolescents aged 12–15 years in 71 low- and middle-income countries (LMIC) from the GSHS with cross-sectional design were included in this study. Loneliness was defined as feeling lonely sometimes or more frequently in the past 12 months. Parental smoking, reported by the adolescent, was defined as the use of any form of tobacco by the father and/or mother. Logistic regression models were used to examine the odds ratio (OR) of adolescent loneliness according to parental smoking status.ResultsCompared with neither parent smoking, parental smoking was associated with adolescent loneliness (father only: OR = 1.18, 95% CI = 1.10–1.26; mother only: OR = 1.39, 95% CI = 1.15–1.67; both: OR = 1.65, 95% CI = 1.41–1.93) after adjustment for potential covariates. Subgroup analyses stratified by sex, age, and WHO region showed similar results (except not in the African and Western Pacific regions).DiscussionParental smoking is associated with loneliness in adolescents from LMIC. Our finding expands the well-known physical damage of parental smoking in adolescents to the psychological damage. Strict policies and strategies should be established to encourage smoking cessation for parents who are current smokers.
Growth and Rhamnolipid Production Performance of Pseudomonas aeruginosa on Crude Biomass Carbohydrates and Bioenhancer-Based Growth Media
Authors: Sharma Rajat , Lamsal Buddhi P. 
Published: February 2025

Novel growth media formulations for improved rhamnolipid production from Pseudomonas aeruginosa PAO1 were evaluated on four carbohydrate sources: glucose, glycerol, soy hull hydrolysate (SHH), and mimicking soy hull hydrolysate (MSH) along with bioenhancers and other media components. This study is aimed at understanding the effect of different types of human neuroendocrine bioenhancers on growth performance and rhamnolipid titer generation of the Pseudomonas aeruginosa PAO1 in a growth media containing sustainable crude biomass carbohydrates. Optimization of the media factors for improved rhamnolipid titers with Pseudomonas aeruginosa PAO1 was performed through a high-throughput response surface study for the best growth rate for concentrations of carbohydrates; bioenhancers, norepinephrine (NE) and dopamine (DP); and iron (Fe). In the high-throughput study, the microbial growth rates for all sugar types ranged between 0.2 and 0.5 log numbers in OD (optical density, indicating the concentration of bacterial cells within a liquid culture, as determined by a spectrophotometer) h−1 at 600 nm, with glucose providing the highest growth rate in the best response surface media combination at 2.5% glucose concentration, 160 µM norepinephrine, 66 µM dopamine, 0.03% Fe concentration. The effect of this media on growth and rhamnolipid production was further verified in 100 mL shake flasks. The highest OD and rhamnolipid titers were achieved for glucose- and glycerol-based media at 2.78 g/L and 2.72 g/L, respectively, whereas significantly lower titers at 1.98 g/L and 1.72 g/L were observed for SHH- and MSH-based media, respectively. No significant growth enhancement effects by the bioenhancers norepinephrine and dopamine were observed at the concentrations evaluated.
Identification of Grape NRT Gene Family and Analysis of Its Expression in Leaves Under Nitrogen-Deficiency Stress
Authors: Yang Zhongyi , Mei Junjie , Zheng Wei , Khan Falak Sher , Bhuiyan Mohammad Nasim , Wang Kangjie , Rhaman Mohammad Saidur , Abe-Kanoh Naomi , Ji Wei 
Published: February 2025

Nitrogen, an indispensable macronutrient, significantly affects the appearance, quality, and yield of grapes (Vitis vinifera). Adequate nitrate uptake and intracellular transport, facilitated primarily by nitrate transport (NRT) proteins, are crucial for maintaining nutritional balance. However, there are no reports on the NRT gene family in grapes. In this study, we identified 53 Nitrate Transporter 1/Peptide Transporter Family (NPF), 3 nitrate transporter 2 family (NRT2), and 1 Nitrate Assimilation-Related 2 (NAR2) genes in the grapevine Pinot Noir PN40024 genome. A comprehensive analysis of these gene families, including their physicochemical properties, structural organization, chromosomal distribution, collinearity, cis-acting element distribution, and phylogenetic relationships, revealed the rich diversity and evolutionary conservation of the grapevine Nitrate Transporter (NRT) genes. Furthermore, the expression profiles of VvNRTs in different tissues demonstrated that the NRT genes possess spatio-temporal expression specificity. The expression patterns of the NRT genes were examined by transcriptome sequencing in grapevines across various tissues under nitrogen-deficiency conditions. The expression patterns in grapevine leaves under nitrogen-deficiency conditions showed upregulation of the VvNPF2.3 gene in conditions of nitrogen deficiency. This upregulation was strongly associated with a 62.2% reduction in indole-3-acetic acid (IAA) levels and a 21.3% increase in hydrogen peroxide (H2O2) levels, suggesting a complex regulatory response to nitrogen-induced stress. These findings emphasize the potential involvement of NRT genes in the adaptive reaction to nitrogen deficiency and set the stage for future investigations into the molecular mechanisms of nitrogen transportation in grapevines.
Unveiling the Kadaknath Gut Microbiome: Early Growth Phase Spatiotemporal Diversity
Authors: Nair Amruta , Doijad Swapnil Prakash , Suryavanshi Mangesh Vasant , Dey Anwesha , Singh Malik Satya Veer , Dutilh Bas E. , Barbuddhe Sukhadeo Baliram 
Published: February 2025

The early growth phase is a critical period for the development of the chicken gut microbiome. In this study, the spatiotemporal diversity of the gastrointestinal microbiota, shifts in taxonomic composition, and relative abundances of the main bacterial taxa were characterized in Kadaknath, a high-value indigenous Indian chicken breed, using sequencing of the V3–V4 region 16S rRNA gene. To assess microbiome composition and bacterial abundance shifts, three chickens per growth phase (3, 28, and 35 days) were sampled, with microbiota analyzed from three gut regions (crop, small intestine, and ceca) per bird. The results revealed Firmicutes as the most abundant phylum and Lactobacillus as the dominant genus across all stages. Lactobacillus was particularly abundant in the crop at early stages (3 and 28 days), while the ceca exhibited a transition towards the dominance of genus Phocaeicola by day 35. Microbial richness and evenness increased with age, reflecting microbiome maturation, and the analyses of the microbial community composition revealed distinct spatiotemporal differences, with the ceca on day 35 showing the highest differentiation. Pathogen analysis highlighted a peak in poultry-associated taxa Campylobacter, Staphylococcus, and Clostridium paraputrificum in 3-day-old Kadaknath, particularly in the small intestine, underscoring the vulnerability of early growth stages. These findings provide critical insights into age-specific microbiome development and early life-stage susceptibility to pathogens, emphasizing the need for targeted interventions to optimize poultry health management and growth performance.
Religião e espiritualidade: seus impactos em pacientes em cuidados paliativos: uma revisão integrativa
Authors: Lemos Carolina Teles , Souza Ramos Rodrigo 
Published: February 2025

As doenças crônicas passam por um processo de aumento em escala mundial, o que torna necessário a implementação de um modelo de assistência que contemple todo o processo de adoecimento, a morte e o morrer. Nessa ótica, os cuidados paliativos fazem parte de uma modalidade emergente da assistência integral e multidisciplinar, contemplando, inclusive, a espiritualidade do paciente com doença avançada e terminal. Nosso objetivo é apresentar uma revisão bibliográfica feita para identificar o impacto da religião e da espiritualidade no seguimento de pacientes em cuidados paliativos. Como metodologia, realizamos uma Revisão Bibliográfica Integrativa de artigos científicos publicados nas principais bases de dados atuais – PubMed, Scielo e Biblioteca Virtual em Saúde (BVS). Os critérios de inclusão foram trabalhos publicados nos últimos dez anos (entre 2013 e 2023). O universo do estudo foi constituído por 376 artigos relacionados ao tema abordado, dos quais 15 compuseram à amostra por se adequarem aos critérios de inclusão. Como resultados, percebemos que os estudos analisados apontaram para a compreensão que no caso de pacientes em cuidados paliativos, essas ferramentas se configuram como um caminho para que esses indivíduos possam lidar com a terminalidade, sem angústia, reduzindo o sofrimento e a dor provocados pelas doenças incuráveis.
Influence of temperature on growth and pathogenicity of Gobisporangium ultimum, G. irregulare and Pythium aphanidermathum that cause Soybean Damping Off in Buenos Aires (Argentina)
Authors: Enrique Grijalba Pablo , Alberto Pucheta Juan 
Published: February 2025

Damping off of soybean, caused by Pythium spp., is favored by cool temperatures and wet soil conditions. The goal of this research was to determine the effect of three temperatures on growth and pathogen aggressiveness of three different species of Pythium (s.l.) associated with soybean in the Buenos Aires province. A total of 12 isolates of the most prevalent Pythium spp. in Buenos Aires were screened. Daily growth rate in culture medium was measured and seed and seedling assays were performed. Seed and seedling assays were used to quantify the aggressiveness of Globisporangium ultimum, G. irregulare and P. aphanidermatum on soybean at 15, 25, and 35°C. G. ultimum was more aggressive at 15°C compared with 25 and 35°C. Conversely, P. aphanidermatum was more aggressive at 35°C than at 15 and 25°C. Our data showed that aggressiveness of some species of Pythium on soybean was influenced by temperature, which suggests that future studies evaluating pathogenicity of this pathogen should be performed at more than one temperature.
Uji Aktivitas Antibakteri dan Antibiofilm Fraksi Ekstrak Daun Gamal (Gliricidia Sepium (Jacq Walp)) Terhadap Staphylococcus Aureus ATCC 25923
Authors: Herman Herman , Sunarni Titik , Saptarini Opstaria 
Published: February 2025

Daun gamal (Gliricidia sepium (Jacq Walp)) di dalamnya terkandung zat flavonoid, alkaloid, tanin, saponin, dan terpenoid. Senyawa dalam daun gamal mempunyai sifat uang antibakteri. Tujuan dari penelitian ini yakni untuk mengetahui bagaimana peran ekstrak serta fraksi n-heksana, etil asetat, serta air terhadap aktivitas antibakteri dengan menghambat pembentukan dan degradasi biofilm. Metode maserasi digunakan untuk mengekstraksi daun gamal menggunakan pelarut etanol 96%. Ekstrak kental dilakukan fraksinasi dengan pelarut n-heksana, air, serta etil asetat. Uji aktivitas antibakteri dilaksanakan melalui metode dilusi dilanjutkan observasi pengaruh kerja melalui uji AAS kebocoran ion. Uji aktivitas antibiofilm menggunakan alat microplate serta pembacaannya dilaksanakan pada panjang gelombang 595 nm. Hasil penelitian bahwa fraksi etil asetat daun gamal teraktif terhadap bakteri S. aureus. Fraksi etil asetat daung gamal dapat merusak pada membran sel bakteri. Rata-rata persentase penghambatan pembentukan biofilm terhadap S. aureus terbesara di konsentrasi 50%, yaitu fraksi etil asetat sebanyak 40,69%, ekstrak 37,51%, fraksi n-heksana 28,93% dan fraksi air 30,20%, sedangkan rata-rata persentase degradasi biofilm terlihat yang tertinggi adalah fraksi etil asetat dengan konsentrasi 50% yaitu sebesar 37,27%, ekstrak 34,55%, fraksi air 31,77%, fraksi n-heksan nilai 30,05%. Kontrol positif (Tetrasiklin) memiliki aktivitas dengan nilai 51,66 %.
Mechanism Of Alpha Mangosteen As An Inhibitor Of Matrixmetalloproteinase-1 In Skin Cancer By In-Silico Study
Authors: Fristiohady Adryan , Arfan Arfan , Asasutjarit Rathapon , Haruna Lidya Agriningsih , Purnama La Ode Muhammad Julian , Sahidin Idin , Malik Fadhliyah , Kirana Rezky Marwah , Kardin Laode , Idrus Loly Subhiaty , Malaka Muhammad Hajrul 
Published: February 2025

?-mangostin (?-MG) is a metabolite of xanthones isolated from fruit of Garcinia mangostana Linn pericarps. Skin cancer is one of the most common malignancies affecting many people. The development of skin cancer is mainly due to intermittent or long-term exposure to ultraviolet (UV) radiation resulting in reduced cell-mediated immune response, production of reactive oxygen species (ROS), and DNA changes. Increased levels of ROS can induce the production of matrix metalloproteinases (MMPs). ?-MG showing potential as an anti-metastatic agent with reduced the expression of MMP-1. This study aims to determine the affinity and stability profiles of ?-MG as anti-skin cancer by applying the In-Silico method. Molecular simulation of ?-MG was successfully docked to the MMP-1. ?-MG showed stable results after 100 ns molecular dynamics simulation based on the root mean square deviation (RMSD) and the root mean squared fluctuation (RMSF). The binding energies of the xanthone derivatives were calculated using the MM/PBSA method.
Pengaruh Ekstrak Etanol 96 % Daun Asam Jawa terhadap Kadar Katalase Darah Mencit yang Dipapar Asap Rokok
Authors: Saputri Gusti Ayu Rai , Putri Restika Ananda , Hermawan Dessy 
Published: February 2025

Asap rokok mengandung bahan kimia toksik yang berperan sebagai pemicu pembentukan radikal bebas dan berujung pada keadaan stress oksidatif yang ditandai dengan penurunan kadar katalase darah. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 96% daun Asam Jawa (Tamarindus indica L.) dengan variasi dosis 150, 200, dan 250 mg/25 gBB terhadap kadar katalase darah mencit yang dipapar asap rokok. Metode penelitian ini yaitu eksperimental dengan pendekatan Pre and post control group design. Hewan uji dibagi menjadi 6 kelompok perlakuan, yaitu K 1 (hanya diberi pakan dan minum), K 2 perlakuan negatif (diberi paparan asap rokok dan CMC-Na 0,5%), K 3 perlakuan positif (diberi paparan asap rokok dan Vitamin E), K 4, K 5, dan K 6 diberi paparan asap rokok dan ekstrak daun Asam Jawa dosis 150, 200, dan 250 mg/25 gBB. Pengukuran aktivitas katalase dilakukan secara Spektrofotometri pada panjang gelombang 280 nm. Hasil penelitian menunjukan rerata kadar katalase darah pada K 1, K 2, K 3, K 4, K 5 dan K 6 secara berurutan adalah 0,158 U/mg, 0,029 U/mg, 0,220 U/mg, 0,184 U/mg, 0,272 U/mg dan 0,303 U/mg dengan uji One-Way ANOVA didapatkan nilai p=0,001 (p<0.05). Uji post hoc Duncan memiliki perbedaan tingkat signifikansi pada kelompok yang berbeda masing-masing kosentrasi. Semakin besar kosentrasi yang ditambahkan semakin signifikan dampaknya terhadap kadar katalase darah yang dipapar asap rokok.