bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybridization in Large-Bodied New World Primates
Authors: Cortés-Ortiz Liliana , Duda Thomas F , Canales-Espinosa Domingo , García-Orduña Francisco , Rodríguez-Luna Ernesto , Bermingham Eldredge 
Published: July 2007

AbstractWell-documented cases of natural hybridization among primates are not common. In New World primates, natural hybridization has been reported only for small-bodied species, but no genotypic data have ever been gathered that confirm these reports. Here we present genetic evidence of hybridization of two large-bodied species of neotropical primates that diverged ∼3 MYA. We used species-diagnostic mitochondrial and microsatellite loci and the Y chromosome Sry gene to determine the hybrid status of 36 individuals collected from an area of sympatry in Tabasco, Mexico. Thirteen individuals were hybrids. We show that hybridization and subsequent backcrosses are directionally biased and that the only likely cross between parental species produces fertile hybrid females, but fails to produce viable or fertile males. This system can be used as a model to study gene interchange between primate species that have not achieved complete reproductive isolation.
Homeostatic Mechanisms for Iron Storage Revealed by Genetic Manipulations and Live Imaging of Drosophila Ferritin
Authors: Missirlis Fanis , Kosmidis Stylianos , Brody Tom , Mavrakis Manos , Holmberg Sara , Odenwald Ward F , Skoulakis Efthimios M C , Rouault Tracey A 
Published: July 2007

Abstract
Ferritin is a symmetric, 24-subunit iron-storage complex assembled of H and L chains. It is found in bacteria, plants, and animals and in two classes of mutations in the human L-chain gene, resulting in hereditary hyperferritinemia cataract syndrome or in neuroferritinopathy. Here, we examined systemic and cellular ferritin regulation and trafficking in the model organism Drosophila melanogaster. We showed that ferritin H and L transcripts are coexpressed during embryogenesis and that both subunits are essential for embryonic development. Ferritin overexpression impaired the survival of iron-deprived flies. In vivo expression of GFP-tagged holoferritin confirmed that iron-loaded ferritin molecules traffic through the Golgi organelle and are secreted into hemolymph. A constant ratio of ferritin H and L subunits, secured via tight post-transcriptional regulation, is characteristic of the secreted ferritin in flies. Differential cellular expression, conserved post-transcriptional regulation via the iron regulatory element, and distinct subcellular localization of the ferritin subunits prior to the assembly of holoferritin are all important steps mediating iron homeostasis. Our study revealed both conserved features and insect-specific adaptations of ferritin nanocages and provides novel imaging possibilities for their in vivo characterization.
Enzyme Replacement Therapy in Patients Who Have Mucopolysaccharidosis I and Are Younger Than 5 Years: Results of a Multinational Study of Recombinant Human α-l-Iduronidase (Laronidase)
Authors: Wraith J. Edmond , Beck Michael , Lane Roderick , van der Ploeg Ans , Shapiro Elsa , Xue Yong , Kakkis Emil D. , Guffon Nathalie 
Published: July 2007

OBJECTIVE. Our objective was to evaluate the safety, pharmacokinetics, and efficacy of laronidase in young, severely affected children with mucopolysaccharidosis I.
METHODS. This was a prospective, open-label, multinational study of 20 patients who had mucopolysaccharidosis I and were &lt;5 years old (16 with Hurler syndrome, 4 with Hurler-Scheie syndrome) and were scheduled to receive intravenous laronidase at 100 U/kg (0.58 mg/kg) weekly for 52 weeks. Four patients underwent dosage increases to 200 U/kg for the last 26 weeks because of elevated urinary glycosaminoglycan levels at week 22.
RESULTS. Laronidase was well tolerated at both dosages. Investigators reported improved clinical status in 94% of patients at week 52. The mean urinary glycosaminoglycan level declined by ∼50% at week 13 and was sustained thereafter. A more robust decrease in urinary glycosaminoglycan was observed in patients with low antibody levels and those who were receiving the 200 U/kg dosage. On examination, the liver edge was reduced by 69.5% in patients with a palpable liver at baseline and week 52 (n = 10). The proportion of patients with left ventricular hypertrophy decreased from 53% to 17%. Global assessment of sleep studies showed improvement or stabilization in 67% of patients, and the apnea/hypopnea index decreased by 5.8 events per hour (−8.5%) in those with abnormal baseline values. The younger patients with Hurler syndrome (&lt;2.5 years) and all 4 patients with Hurler-Scheie syndrome showed normal mental development trajectories during the 1-year treatment period.
CONCLUSIONS. Laronidase seems to be well tolerated and to provide clinical benefit in patients who have severe mucopolysaccharidosis I and are &lt;5 years old. Enzyme replacement therapy is not curative and may not improve all affected organs and systems in individuals when irreversible changes have developed. The long-term clinical outcome and effects of antibodies and laronidase dosing on glycosaminoglycan reduction warrant additional investigation.
Cost of Hospitalization for Preterm and Low Birth Weight Infants in the United States
Authors: Russell Rebecca B. , Green Nancy S. , Steiner Claudia A. , Meikle Susan , Howse Jennifer L. , Poschman Karalee , Dias Todd , Potetz Lisa , Davidoff Michael J. , Damus Karla , Petrini Joann R. 
Published: July 2007

OBJECTIVE. The objective of this study was to estimate national hospital costs for infant admissions that are associated with preterm birth/low birth weight.METHODS. Infant (&lt;1 year) hospital discharge data, including delivery, transfers, and readmissions, were analyzed by using the 2001 Nationwide Inpatient Sample from the Healthcare Cost and Utilization Project. The Nationwide Inpatient Sample is a 20% sample of US hospitals weighted to approximately &gt;35 million hospital discharges nationwide. Hospital costs, based on weighted cost-to-charge ratios, and lengths of stay were calculated for preterm/low birth weight infants, uncomplicated newborns, and all other infant hospitalizations and assessed by degree of prematurity, major complications, and expected payer.RESULTS. In 2001, 8% (384200) of all 4.6 million infant stays nationwide included a diagnosis of preterm birth/low birth weight. Costs for these preterm/low birth weight admissions totaled $5.8 billion, representing 47% of the costs for all infant hospitalizations and 27% for all pediatric stays. Preterm/low birth weight infant stays averaged $15100, with a mean length of stay of 12.9 days versus $600 and 1.9 days for uncomplicated newborns. Costs were highest for extremely preterm infants (&lt;28 weeks’ gestation/birth weight &lt;1000 g), averaging $65600, and for specific respiratory-related complications. However, two thirds of total hospitalization costs for preterm birth/low birth weight were for the substantial number of infants who were not extremely preterm. Of all preterm/low birth weight infant stays, 50% identified private/commercial insurance as the expected payer, and 42% designated Medicaid.CONCLUSIONS. Costs per infant hospitalization were highest for extremely preterm infants, although the larger number of moderately preterm/low birth weight infants contributed more to the overall costs. Preterm/low birth weight infants in the United States account for half of infant hospitalization costs and one quarter of pediatric costs, suggesting that major infant and pediatric cost savings could be realized by preventing preterm birth.
Evaluating Deliberation in Pediatric Primary Care
Authors: Cox Elizabeth D. , Smith Maureen A. , Brown Roger L. 
Published: July 2007

OBJECTIVE. Patient participation during decision-making can improve health outcomes and satisfaction, even for routine pediatric concerns. The tasks that are involved in decision-making include both information exchange and deliberation about potential options, yet deliberation (ie, the process of expressing and evaluating potential options to reach a decision) is often assessed subjectively, if at all. We objectively assessed the amount of deliberation; the involvement of parents and children in deliberation; and how deliberation is associated with child, physician, parent, and visit characteristics.METHODS. From videotapes of 101 children's acute care visits to 1 of 15 physicians, we coded the speaker, recipient, and timing of proposed plans (ie, options) and agreements or disagreements with the plans. Reliability of measures was assessed with Cohen's κ or intraclass correlation coefficients; validity was assessed with Spearman correlations. Outcome measures included number of plans proposed, deliberation length, and parent/child involvement in deliberation as either active (child or parent proposed a plan or disagreed with a plan) or passive (physician alone proposed plans). Multivariable models that accounted for clustering by physician were used to relate child, physician, parent, and visit factors to deliberation measures.RESULTS. The mean number of plans proposed was 4.1, and deliberation time averaged 2.9 minutes per visit. Passive involvement of parents/children occurred in 65% of visits. After adjustment, more plans were proposed in visits by girls, and shorter deliberations occurred with college-graduate parents. Longer visits were associated with more plans proposed, longer deliberation, and reduced odds for passive parent/child involvement.CONCLUSIONS. Using a reliable and valid technique, deliberation was demonstrated to occupy a substantial portion of the visit and include multiple proposed plans, yet passive involvement of parents and children predominated. Results support the need to develop interventions to improve parent and child participation in deliberation.
Hydrops Fetalis: A Retrospective Review of Cases Reported to a Large National Database and Identification of Risk Factors Associated With Death
Authors: Abrams Matthew E. , Meredith Keith S. , Kinnard Paula , Clark Reese H. 
Published: July 2007

OBJECTIVES. The objectives were (1) to identify the causes for hydrops fetalis neonates admitted for neonatal intensive care with the diagnosis of hydrops fetalis and (2) to identify the risk factors associated with death.
METHODS. A retrospective review of a large national data set was performed.
RESULTS. There were a total of 253651 discharges from 162 NICUs in the database; 598 patients were identified with a report of hydrops fetalis. The most common associated diagnoses were congenital heart problems (13.7%), abnormalities in heart rate (10.4%), twin-to-twin transfusion (9%), congenital anomalies (8.7%), chromosomal abnormalities (7.5%), congenital viral infections (6.7%), congenital anemia (5%), and congenital chylothorax (3.2%). Of those 598 neonates, 115 were transferred either to another hospital or to another service, 215 died before discharge, and 267 were discharged from the hospital. One patient did not have a discharge type listed and was not included in the outcome analysis. Mortality rates were highest among neonates with congenital anomalies (57.7%) and lowest among neonates with congenital chylothorax (5.9%). Factors that were associated independently with death in logistic regression analyses were younger gestational age, low 5-minute Apgar score, and need for high levels of support during the first day after birth (higher levels of inspired oxygen support and more often treated with high-frequency ventilation).
CONCLUSIONS. The risk of death among neonates with hydrops fetalis depends on the underlying diagnosis and is highest for those who are born more prematurely and those who are most ill immediately after birth. Information from this large study should prove useful for planning prospective studies and providing prenatal counseling to parents with an affected fetus.
Sources and handling of losses to follow-up in parallel-group randomized clinical trials in dogs and cats: 63 trials (2000–2005)
Authors: Brown Dorothy Cimino 
Published: July 2007

Abstract
Objective—To determine the sources and handlingof losses to follow-up (LTF) in parallel-group randomized clinical trials (RCTs).
Sample Population—63 parallel-group RCTs of > 24 hours' duration published from January 2000 through December 2005.
Procedures—Journals were hand searched for eligible reports. Details concerning the presence, cause, and amount of LTF; statistical handlingof data missingbecause of LTF; type of analyses performed; number of animals randomly allocated and analyzed; and the acknowledgement of the potential impact of LTF were recorded.
Results—In 81% (51/63) of trials, LTF were reported. In 80% (41/51) of those studies, losses in the analysis were ignored, and in only 18% (9/51) was the potential impact of LTF on study results acknowledged. Of the 47 studies in which sources of LTF were reported, 72% had loss of subjects because of investigator withdrawals, 30% because of deaths, and 26% because of owner withdrawals. Median loss of subjects for those studies was 12% because of investigator withdrawal (range, 2% to 52%), 8% because of death (1% to 28%), and 4% because of owner withdrawal (2% to 33%).
Conclusions and Clinical Relevance—Most RCTs had LTF, most of which were attributable to investigators removing randomly allocated animals from the study. In most studies, data from animal LTF were ignored and, therefore, only a subgroup of randomly allocated subjects was included in the data analysis. Most reports did not address the potential for a postrandomization selection bias associated with ignoring LTF and did not acknowledge the potential impact of the missingdata on their results.
Effect of voltage-gated and capacitative calcium entry blockade on agonist-induced constriction of equine laminar blood vessels
Authors: Peroni John F. , Moore James N. , Noschka Erik , Lewis Tristan H. , Lewis Stephen J. , Robertson Tom P. 
Published: July 2007

Abstract
Objective—To characterize the relative contributions of voltage-gated and capacitative Ca2+ entry to agonist-induced contractions of equine laminar arteries and veins.
Animals—16 adult mixed-breed horses.
Procedures—Laminar arteries and veins were isolated and mounted on small vessel myographs for the measurement of isometric tension. Concentration-response curves were obtained for the vasoconstrictor agonists phenylephrine, 5-hydroxytryptamine (5-HT), prostaglandin F2α (PGF2α), and endothelin-1 (ET-1) either in the absence of extracellular Ca2+ or in the presence of the voltage-gated Ca2+ channel inhibitor diltiazem or the putative inhibitor of capacitative Ca2+ entry, trifluoromethylphenylimidazole.
Results—In the absence of extracellular Ca2+, maximal responses of veins to 5-HT, phenylephrine, ET-1 and PGF2α were reduced by 80%, 50%, 50%, and 45%, respectively; responses of arteries to 5-HT, phenylephrine, and ET-1 were reduced by 95%, 90%, and 20%, respectively. Although diltiazem did not affect the maximal responses of veins to any agonist, responses of arteries to 5-HT, phenylephrine, and ET-1 were reduced by 40%, 50%, and 27%, respectively. Trifluoromethylphenylimidazole did not affect maximal responses of veins, but did reduce their contractile responses to low concentrations of ET-1 and PGF2α.
Conclusions and Clinical Relevance—Results suggested that the contribution of extracellular Ca2+ to laminar vessel contractile responses differs between arteries and veins and also between contractile agonists, voltage-gated Ca2+ entry is more predominant in laminar arteries than in veins, and capacitative Ca2+ entry has a minor role in agonist-induced contractile responses of laminar veins.
Addition of chromic oxide to creep feed as a fecal marker for selection of creep feed–eating suckling pigs
Authors: Kuller Wikke I. , van Beers-Schreurs Hetty M. G. , Soede Nicoline M. , Taverne Marcel A. M. , Kemp Bas , Verheijden Jos H. M. 
Published: July 2007

Abstract
Objective—To determine whether the addition of chromic oxide (Cr2O3) to creep feed could be used as a visual marker in feces for selection of creep feed–eating suckling pigs.
Animals—20 suckling pigs.
Procedures—Via syringe, 5 pigs (2 to 3 days old on day 0; 1 pig/treatment) from each of 4 litters received oral administrations of 10, 20, 30, or 40 g of creep feed containing 10 g of Cr2O3•kg−1 on each of 2 consecutive days (days 20 and 21) or 30 g of creep feed containing 10 g of Cr2O3•kg−1 on day 20 and 30 g of Cr2O3-free creep feed on day 21. On days 21 through 24, 6 fecal samples were collected from each pig at regular intervals between 8:00 AM and 6:00 PM. Green-colored feces were considered indicative of creep feed consumption (eaters). Data analyses were based on single and multiple fecal samples.
Results—On day 22, evaluation of 1 fecal sample/pig and multiple fecal samples per pig resulted in identification of as many as 40% and only 15% of the feed-treated pigs wrongly as noneaters, respectively. Repeated sampling over multiple days would identify 99% of eaters accurately. Pigs erroneously identified as noneaters were those administered either low amounts of Cr2O3-supplemented creep feed for 2 days or Cr2O3-supplemented creep feed on only 1 day.
Conclusions and Clinical Relevance—Data suggest that addition of Cr2O3 to creep feed enables selection of individual creep feed–eating suckling pigs via examination of feces, provided that repeated fecal samples are evaluated.
Effect of maternal cells transferred with colostrum on cellular responses to pathogen antigens in neonatal calves
Authors: Donovan Douglas C. , Reber Adrian J. , Gabbard Jon D. , Aceves-Avila Maria , Galland Kimberly L. , Holbert Katheryn A. , Ely Lane O. , Hurley David J. 
Published: July 2007

Abstract
Objective—To assess the effect of maternal cells or cellular components on neonatal immune responses to intracellular pathogens in calves.
Animals—15 Holstein calves.
Procedures—Calves were fed whole colostrum, frozen colostrum, or cell-free colostrum within 4 hours after birth. Leukocytes were obtained from calves before feeding colostrum and 1, 2, 7, 14, 21, and 28 days after ingestion. Proliferative responses against bovine viral diarrhea virus (BVDV) and mycobacterial purified protein derivatives were evaluated. Dams received a vaccine containing inactivated BVDV, but were not vaccinated against mycobacterial antigens.
Results—All calves had essentially no IgG in circulation at birth, but comparable and substantial concentrations by day 1. Calves that received whole colostrum had enhanced responses to BVDV antigen 1 and 2 days after ingestion of colostrum. In contrast, calves that received frozen colostrum or cell-free colostrum did not respond to BVDV. No differences were identified among the 3 groups in response to mycobacterial antigens.
Conclusions and Clinical Relevance—Results indicated that transfer of live maternal cells from colostrum to neonatal calves enhanced responses to antigens against which the dams had previously responded (BVDV), but not to antigens to which the dams were naïve (mycobacterial purified protein derivatives). Results suggested that cell-mediated immune transfer to neonates can be enhanced by maternal vaccination.
Clinical signs, treatments, and outcome in cats with transitional cell carcinoma of the urinary bladder: 20 cases (1990–2004)
Authors: Wilson Heather M. , Chun Ruthanne , Larson Victoria S. , Kurzman Ilene D. , Vail David M. 
Published: July 2007

Abstract
Objective—To characterize demographics and clinical signs and evaluate outcomes of treatments in cats with transitional cell carcinoma (TCC) of the urinary bladder.
Design—Retrospective case series.
Animals—20 cats with TCC.
Procedures—Medical records of 20 cats with a bladder mass identified as a TCC that were examined at 2 veterinary institutions between 1990 and 2004 were evaluated. Signalment, treatments, and outcome were assessed.
Results—Breeds included domestic short hair (n = 14), long hair (2), and medium hair (2) cats, Siamese (1), and Abyssinian (1). All cats had been neutered at an early age (< 1 year old; 13 neutered males and 7 spayed females). The median age at diagnosis of TCC was 15.2 years. The trigone region was affected in 9 cats. Treatments included piroxicam administration, chemotherapy, or surgery as single interventions or in combination; 6 cats were not treated. At the time of diagnosis, 3 cats had pulmonary metastasis and 1 cat had metastasis to local lymph nodes. Median survival time for all 20 cats was 261 days. Nearly all deaths were attributable to progressive disease in the urinary tract. Five cats were lost to follow-up.
Conclusions and Clinical Relevance—In cats, TCC of the urinary bladder appears to be a rare and aggressive disease that is more prevalent in male cats and frequently develops at sites distant from the trigone (unlike TCC in dogs). Nevertheless, initial clinical signs of TCC in cats in this study were similar to those reported for affected dogs.
ICP slurry introduction for simple and rapid determination of Pb, Mg and Ca in plant roots
Authors: Barałkiewicz Danuta , Kanecka-Hanc Anetta , Gramowska Hanka 
Published: July 2007

AbstractPb, Mg and Ca were simultaneously determined in plant roots by slurry introduction into inductively coupled plasma optical emission spectrometry (SS-ICP-OES). Slurries were prepared in 0.5% or 5% (v/v) HNO3 with 0.5, or 5% (v/v) Triton X-100. Omission of the Triton X-100 improved results. Compared with wet ashing of the root sample followed by ICP-OES, ICP-MS and FAAS, the method offers: comparable results, simplification of sample preparation, less sample contamination, and reduction in the use of dangerous and corrosive reagents. The precisions varied: 1.7% for Mg, 2.8% for Ca and 4.3% for Pb, and were not significantly different (95% confidence level) from those of conventional analysis.
The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea
Authors: Luo Yan , Tang Yuedi , Xia Qingjie , Liu Jin 
Published: July 2007

AbstractEndothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea.