bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accuracy of magnetic resonance imaging compared with computed tomography for implant planning
Authors: Aguiar Marcelo F. , Marques Alexandre P. , Carvalho Antonio Carlos P. , Cavalcanti Marcelo G.P. 
Published: February 2008

AbstractAim: The objective of this study was to evaluate the reliability of magnetic resonance imaging (MRI) compared with computed tomography (CT) for dental implant planning in respect to bone measurements.Methods: Five dry mandibles were submitted to MRI and CT examinations. Each mandible had three specific sites identified with markers, in a total of 15 sites to be studied. The images provided by the MRI and CT examinations were delivered to four specialists in Oral and Maxillofacial Radiology to measure the bone height at the specific sites. Subsequently, the bone height of the dry mandibles was directly measured in the determined sites. The measurements obtained by the specialists in MRI and CT images were compared with the measurements obtained directly from the dry mandibles using the ANOVA test with a 0.05 significance level.Results: The differences between the measurements obtained directly from the dry mandibles and the measurements from the MRI exams varied from 0.13 to 1.67 mm, with 10 sites being overestimated in MRI exams and five sites being underestimated. The differences between the measurements from the dry mandibles and CT exams varied from 0.02 to 1.25 mm, with nine sites being overestimated in CT exams and six sites being underestimated. The differences between the measurements from the MRI and CT exams varied from 0.03 to 1.00 mm, with nine sites giving higher values in MRI exams and six sites giving higher values in CT exams. There were no statistically significant differences between the three grous of measurements (P=0.98).Conclusion: The MRI, when compared with CT, shown to be reliable in respect to bone measurements for dental implant planning.
The dimensions of keratinized mucosa around implants affect clinical and immunological parameters
Authors: Zigdon Hadar , Machtei Eli E. 
Published: February 2008

AbstractObjectives: To investigate the association between keratinized mucosa (KM) width and mucosal thickness (MTh) with clinical and immunological parameters around dental implants.Methods: Sixty‐three functioning dental implants (3I osseotite) were examined. Clinical examinations included plaque index (PI), probing depth (PD), bleeding on probing (BOP), KM width, MTh and buccal mucosal recession (MR). Peri‐implant crevicular fluid (PICF) samples were collected for PgE2 assay.Results: KM width ranged from 0 to 7 mm (mean 2.5±2), MTh ranged from 0.38 to 2.46 mm (mean 1.11±0.4) and the mean MR was 0.62 mm, ranging from 0 to 3 mm.A negative correlation was found between MTh and MR (r=−0.32, P=0.01); Likewise, KM width showed a negative correlation with MR, periodontal attachment level (PAL) and PgE2 levels (r=−0.41, P<0.001; r=−0.26, P=0.04; r=−0.26, P=0.04, respectively). In contrast, a positive correlation was found between KM width and PD (r=0.27, P=0.03).When data were dichotomized by KM width, a wider mucosal band (>1 mm) was associated with less MR compared with narrow (≤1 mm) band (0.27 and 0.9 mm, respectively, P=0.001). A wider KM band was also associated with a greater PD (3.13 mm) compared with a narrow band (2.66 mm, P=0.04).Similarly, a thick mucosa (≥1 mm) was associated with lesser recession compared with a thin (<1 mm) mucosa (0.45 and 0.9 mm, respectively, P=0.04).Conclusion: The KM around dental implants affects both the clinical and the immunological parameters at these sites. These findings are of special importance in the esthetic zone, where thin and narrow KM may lead to a greater MR.
Bone level alterations at implants placed in the posterior segments of the dentition: outcome of submerged/non‐submerged healing. A 5‐year multicenter, randomized, controlled clinical trial
Authors: Cecchinato Denis , Bengazi Franco , Blasi Giorgio , Botticelli Daniele , Cardarelli Ian , Gualini Federico 
Published: February 2008

AbstractObjective: To determine if longitudinal bone level change at Astra Tech™ implants placed in the posterior part of the dentition was influenced by the healing conditions provided following implant placement, i.e., submerged or non‐submerged healing.Material and methods: Eighty‐four patients and 115 fixed partial dentures (FPDs or cases) entered the study. The cases were randomized into two implant installation groups: initially non‐submerged (group A) or initially submerged (group B) implants. Three hundred and twenty‐four implants were installed (group A=153; group B=171): 145 in the maxilla and 179 in the mandible. Radiographs from the implant sites were obtained at FPD insertion (baseline) and subsequently every 12 months. In the radiographs, the position of the marginal bone at the mesial and distal aspects of the implants was determined and the radiographic (Rx) bone level change over time was calculated.Results: Seven implants failed to integrate (four in group A and three in group B). During the 5 years of monitoring, three implants had to be removed and 35 implants were lost to follow‐up. The Rx bone level alteration that occurred during year 1 was 0.02±0.38 mm in group A and 0.17±0.51 mm in group B. During the subsequent 4 years there was some further Rx bone loss in group B (0.02±0.62 mm), while in group A there was some gain of bone (0.07±0.5 mm).Conclusion: The peri‐implant bone level change and number of biological complications that took place during the 5 years was small and unrelated to the surgical protocol used for implant placement.
ORIGINAL ARTICLE: Quantification and Comparison of Toll‐Like Receptor Expression and Responsiveness in Primary and Immortalized Human Female Lower Genital Tract Epithelia
Authors: Herbst‐Kralovetz Melissa M. , Quayle Alison J. , Ficarra Mercedes , Greene Sheila , Rose William A. , Chesson Ralph , Spagnuolo Rae Ann , Pyles Richard B. 
Published: February 2008

ProblemTo better understand innate immune responses to sexually‐transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll‐like receptor (TLR) expression from a population of women is needed.Method of studyTLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration.ResultsTLR1–3 and 5–9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7–9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels.ConclusionTLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.
ORIGINAL ARTICLE: Effect of Leukocyte Therapy on Tumor Necrosis Factor‐Alpha and Interferon‐Gamma Production in Patients with Recurrent Spontaneous Abortion
Authors: Gharesi‐Fard Behrouz , Zolghadri Jaleh , Kamali‐Sarvestani Eskandar 
Published: February 2008

ProblemConsidering the deleterious role of T helper1 (Th1) cells in pregnancy outcome, a successful treatment for recurrent spontaneous abortion (RSA) should be able to make a significant shift away from Th1 responses. Although paternal leukocyte immunization has been used for treatment of RSA for years, because of methodological differences there is no consensus on the mechanism of action and effectiveness of this method.Method of studyTwenty‐five Iranian non‐pregnant women with RSA and 16 non‐pregnant control women with at least two successful pregnancies were included in this study. All cases were followed up after leukocyte therapy for pregnancy outcome. Mononuclear cells from women were co‐cultured with the husband’s mononuclear cells before and after immunotherapy. The levels of tumor necrosis factor‐alpha (TNF‐α) and interferon‐gamma (IFN‐γ) were checked on culture supernatant by enzyme‐linked immunosorbent assay method.ResultsThe mean concentration of TNF‐α was significantly higher in patients compared with that in normal controls (P = 0.0001). After immunotherapy, the TNF‐α level was only significantly decreased in women with successful outcome (P = 0.0001). Immunotherapy also induced a significant reduction in the IFN‐γ level (P = 0.009).ConclusionThe results of this investigation confirm the role of TNF‐α in RSA and propose the assessment of TNF‐α production as a valuable prognostic parameter for the prediction of abortion after leukocyte therapy.
REVIEW ARTICLE: Guilty As Charged: All Available Evidence Implicates Complement’s Role in Fetal Demise
Authors: Girardi Guillermina 
Published: February 2008

Appropriate complement inhibition is an absolute requirement for normal pregancy. Uncontrolled complement activation in the maternal‐fetal interface leads to fetal death. Here we show that complement activation is a crucial and early mediator of pregnancy loss in two different mouse models of pregnancy loss.Using a mouse model of fetal loss and growth restriction (IUGR) induced by antiphospholipid antibodies (aPL), we examined the role of complement activation in fetal loss and IUGR. We found that C5a‐C5aR interaction and neutrophils are key mediators of fetal injury. Treatment with heparin, the standard therapy for pregnant patients with aPL, prevents complement activation and protects mice from pregnancy complications induced by aPL, and anticoagulants that do not inhibit complement do not protect pregnancies.In an antibody‐independent mouse model of spontaneous miscarriage and IUGR (CBA/J×DBA/2) we also identified C5a as an essential mediator. Complement activation caused dysregulation of the angiogenic factors required for normal placental development. In CBA/J×DBA/2 mice, we observed inflammatory infiltrates in placentas, functional deficiency of free vascular endothelial growth factor (VEGF), elevated levels of soluble VEGF receptor‐1 (sVEGFR‐1, also known as sFlt‐1; a potent anti‐angiogenic molecule), and defective placental development. Inhibition of complement activation blocked the increase in sVEGFR‐1 and rescued pregnancies.Our studies in antibody‐dependent and antibody‐independent models of pregnancy complications identified complement activation as the key mediator of damage and will allow development of new interventions to prevent pregnancy loss and IUGR.
ORIGINAL ARTICLE: N‐glycosylation is Required for Binding of Murine Pregnancy‐Specific Glycoproteins 17 and 19 to the Receptor CD9
Authors: Ha Cam T. , Waterhouse Roseann , Warren James , Zimmermann Wolfgang , Dveksler Gabriela S. 
Published: February 2008

ProblemMurine pregnancy‐specific glycoproteins (PSGs) are encoded by 17 different genes. Different family members have different expression levels at different stages of embryonic development. It is currently unknown whether all members of this family of placentally secreted proteins have the same function and bind to the same receptor. Furthermore, the requirement of post‐translational modifications for the activity of these highly glycosylated proteins remains undetermined.Method of studyRecombinant PSG17 and PSG19 were generated and purified by affinity chromatography. An expression library was screened to identify the receptor for mouse PSG19. Binding to the receptor by proteins generated in different expression systems and mapping of the binding domain were analyzed by pull‐down assays. Analysis of the carbohydrate composition of the receptor‐binding domain was performed with the DIG glycan differentiation kit.ResultsPSG19 binds to the tetraspanin CD9, specifically to extra cellular loop 2 and can induce secretion of TGFβ1 by a macrophage cell line. The receptor‐binding domain of PSG17 and PSG19 is post‐translationally modified by the addition of N‐linked carbohydrates and, when expressed in CHO cells, terminal sialic acids are detected. PSGs produced in bacteria do not bind CD9.ConclusionPSG19, as previously determined for PSG17, binds to the second extracellular loop 2 of the tetraspanin CD9. The first immunoglobulin variable‐like domain of PSG19 is sufficient for receptor binding and function. Analysis of receptor usage by the remaining 15 murine PSGs will most likely require that the proteins be generated in eukaryotic expression systems, as we have demonstrated that the addition of carbohydrates is essential for PSG‐receptor interaction.
ORIGINAL ARTICLE: The Association of RCAS1 Serum Concentration with the Reversibility or Irreversibility of the Process of Immune Cytotoxic Activity Restriction During Normal Menstrual Cycle, Cancer Relapse, and Surgical Treatment for Various Types of Squamous Cell Carcinomas and Adenocarcinomas
Authors: Dutsch‐Wicherek Magda , Wicherek Lukasz 
Published: February 2008

ProblemThe selective suppression phenomenon of the cytotoxic immune response may be brought about by an increase in sRCAS1 serum concentration during the progression of cancer.Method of studyWe studied sRCAS1 serum concentration levels in patients with adenocarcinomas and squamous cell carcinomas. The healthy control consisted of patients who were in the mid‐proliferative or mid‐secretory phases of their cycles.ResultsStatistically, significantly higher sRCAS1 blood serum concentration levels were found in patients experiencing a recurrence of cancer in comparison to those patients who had undergone radical surgical treatment. For patients in the early stages of cancer, sRCAS1 blood serum concentration levels were statistically, significantly higher than those found in patients who were in the proliferative cycle phase and who were comparable to the levels found in patients in the secretory cycle phase.ConclusionThe blood serum concentration level of RCAS1 seems to determine the level of immune system activity restriction that accompanies all three processes—decidualization, cancer development, and cancer relapse.
Polymorphism in ADH and MTHFR genes in oral squamous cell carcinoma of Indians
Authors: Solomon PR , Selvam GS , Shanmugam G 
Published: February 2008

Objectives:  Alcohol consumption is known to increase the risk for several cellular disorders like oral cancer. The risk may be reinforced by polymorphism in genes like alcohol dehydrogenase. Therefore, this study is designed to asses the polymorphic status in ADH1B (formerly ADH2), ADH1C (formerly ADH3) and MTHFR genes in order to correlate the susceptibility to oral squamous cell carcinoma (OSCC).Subjects and methods:  DNA from 126 OSCC samples were amplified using primers for ADH1B, ADH1C and MTHFR genes. The amplicons were analyzed for ADH1B*1, ADH1C*2 and MTHFR C677T allelic polymorphism by restriction digestion using appropriate enzymes.Results:  ADH1B*1/*1 genotype in cancer patients who were heavy drinkers showed a negligible risk association with an odds ratio of 1.62; 95% CI = 1.08–2.14. In OSCC patients, ADH1C*2/*2 genotypes showed a relatively higher risk (odds ratio 2.65; 95% CI = 1.78–3.53) in heavy drinkers and a less significant risk (1.6; 95% CI = 1.15–2.03) in moderate drinkers and negligible risk in light drinkers (1.23; 95% CI = 0.77–1.63). In contrast, MTHFR 677TT genotype showed a high risk association for OSCC in heavy drinkers (odds ratio 3.0; 95% CI = 2.02–4.0). Interestingly, the combination of ADH1B*1/*1/ MTHFR 677TT genotypes in alcoholic cancer patients showed a high risk (odds ratio 4.16; 95% CI = 2.78–5.53). A similar risk (odds ratio 4.16; 95% CI = 1.18–5.53) was shown by ADH1B*1/*2/*2/*2MTHFR 677TT genotype combination. The ADH1C*2/*2 /MTHFR 677TT genotype combination showed the maximum risk (odds ratio 20; 95% CI = 13.45–26.64) in the heavy drinker group. This combination showed a high risk in moderate drinkers (odds ratio 5.88; 95% CI = 4.24–7.50) and relatively lower risk in light drinkers (odds ratio 2.77; 95% CI = 1.74–3.68).Conclusions:  The ADH1C*2/*2/MTHFR 677TT genotype combination appears to be more susceptible for OSCC, since it showed a 20‐fold increase in risk in heavy drinkers and a 5.9‐ and 2.8‐fold increase in risk respectively in moderate drinkers and light drinkers. This study suggests the association of ADH1C*2/*2/MTHFR 677TT genotype combination as a risk factor for OSCC in alcoholics.
Erythema elevatum diutinum with unusual clinical appearance
Authors: Soubeiran Evelyn , Wacker Jörg , Haußer Ingrid , Hartschuh Wolfgang 
Published: February 2008

SummaryErythema elevatum diutinum is a rare chronic inflammatory skin disease of unknown etiology with a histopathology of a leukocytoclastic vasculitis. Clinical findings involve symmetrical livid‐erythematous to red‐brown papules and plaques or nodules which are initially soft but become firmer. The skin lesions are located over extensor surfaces and often over joints. Early treatment with dapsone, corticosteroids and antibiotics is sometimes effective. A patient with multiple myeloma displayed unusual lesions of erythema elevatum diutinum.
Anaphylaxis after mouse bite
Authors: Leng Katharina , Wiedemeyer Katharina , Hartmann Martin 
Published: February 2008

SummaryAllergic sensitivity to laboratory animals is an occupational risk for all workers in contact with small animals. In rare cases, life‐threatening reactions can occur. We describe a laboratory worker who after six years contact with mice developed an anaphylactic reaction after a mouse bite. Testing revealed positive reactions to all components of the mouse. The greatest risk for development of allergy is in the first three years and is usually an immediate hypersensitivity to inhalant allergens.
Cadmium phytoextraction capacity in eight C3 herbage grass species
Authors: Sabreen Shamima , Sugiyama Shu‐ichi 
Published: February 2008

AbstractGrasses are excellent candidates for phytoremediation because of their high biomass production, high adaptability and low management cost. This study assesses interspecific variation of cadmium (Cd) phytoextraction capabilities in eight C3 grass species. Populations of 30‐day‐old C3 grass species – namely, Agrostis alba, Anthoxanthum odoratum, Dactylis glomerata, Festuca arundinacea, Festuca pratensis, Lolium multiflorum, Lolium perenne and Poa pratensis– were grown hydroponically for 15 days with different concentrations of Cd (0, 5, 10 and 50 µM). For each species, shoot biomass, the proportion of growth inhibition (GI, %), shoot Cd concentration and accumulation, shoot nutrient uptake, and the proportion of uptake inhibition (UI, %) of nutrient minerals were evaluated. Effects of Cd application included stunted growth. The GI increased from 5% to 70% with an increase in Cd concentrations. For all Cd treatments, L. multiflorum showed the highest shoot dry biomass. Shoot Cd concentrations negatively affected mineral nutrient uptake. The highest Cd treatment caused UI of various elements of 37–95%. Under 50‐µM Cd treatment, Cd accumulation varied by 20 times among species, and L. multiflorum showed the highest Cd accumulation (116.46 µg plant−1). Our results indicate that L. multiflorum exhibited high degrees of both Cd tolerance and Cd phytoextraction capacity among grass species.
Forage selection by beef cows grazed on a dwarf bamboo (Pleioblastus argenteostriatus f. glaber)‐dominant pasture
Authors: Yayota Masato , Nakano Miwa , Karashima Jun , Ohtani Shigeru 
Published: February 2008

AbstractThis study investigated the influence of forage selection on the quality of the diet ingested by beef cows grazed on a dwarf bamboo‐dominated pasture. For 13 days each in spring, summer and autumn, eight Japanese Black cows were grazed on a 1.8‐ha dwarf bamboo (Pleioblastus argenteostriatus f. glaber)‐dominant pasture during the day. The plant species and number of bites of each species ingested by the cows were recorded, and plant samples were hand plucked to imitate the behavior of the cows during the grazing time on the seventh and 13th days of each period. The botanical and chemical compositions of the available pasture were systematically estimated by sampling 28 plots. The cows slightly avoided dwarf bamboo and preferred grasses on the seventh day of each period. On the 13th day, the cows ate any available forage in the pasture. The selected forages were higher in crude protein and in vitro digestibility and had lower insoluble protein, neutral detergent fiber and lignin contents than the forages available in the pasture during a season. The frequency of the botanical groups selected by the cows was different in autumn from that in spring and summer, and a substantial increase in grasses and a decrease in P. argenteostriatus f. glaber among the selected forages were observed. Although the botanical groups selected by the cows were relatively different between the seasons, an increase in the levels of unavailable fractions such as lignin and insoluble protein and a decrease in in vitro digestibility were observed as the seasons progressed. Thus, the cows could not avoid a decrease in their diet quality in autumn. The selection of forage improved the nutrient intake of the beef cows in terms of chemical and digestible components; however, in a dwarf bamboo‐dominated pasture, there is a substantial limitation in the effectiveness of forage selection.
Effects of chemical composition on the rate and temporal pattern of decomposition in grassland species leaf litter
Authors: Hossain M. Zabed , Sugiyama Shu‐ichi 
Published: February 2008

AbstractWe examined the effects of the chemical composition of leaf litter on the rate and temporal pattern of decomposition for 15 grassland plant species. Respiration rates (CO2 flux) of soil treated with leaf sections of each species were measured periodically at 15 days, 30 days, 45 days and 60 days after incubation under laboratory conditions to monitor temporal changes in decomposition. Results showed that soil respiration rates of the plant species differed significantly at all four measurement times. The soil respiration rate at the earliest measurement (15 days) was positively correlated with the leaf N content, but the rates at the later measurements (30–60 days) were significantly correlated with the phenol : N ratios of litter types, indicating that contribution of litter chemicals changes with progression of decomposition processes. The one‐phase exponential decay curve well represented the temporal changes in the soil respiration rate. The relative rate of decline in soil respiration with time differed significantly among the plant species, and it was correlated significantly and positively with litter N content and negatively with the litter tannin content, indicating importance of litter chemical composition on temporal pattern of decomposition. These results indicate the importance of soil CO2 flux measurements at temporal scale.