Effect of Angelica sinensis /Danggui Wuji Granule on Intestinal Flora in Mice with Aplastic Anemia
Authors: 李 宗源 , Chen Shisheng , Sun Jianhui , Li Xiaomin , Hao Liyu , Yu Zeyue , Li Jianliang , Deng Yurong , Huang Hanhui , Huo Hairu , Li Hongmei 
Published: March 2024

Abstract
Background Angelica sinensis (AS) is a traditional Chinese medical and has a good therapeutic effect on Aplastic anaemia. Danggui Wuji Granule(DGWJ) is a health product with the main effect of treating Aplastic anaemia. It mainly composed of Codonopsis Radix extracts, Ligustrum lucidum extract, Radix paeoniae Alba extract, Angelica extract, lycium barbarum extract, Jujube extract, Pericarpium Citri Reticulate Extracts, Gelatinum Asini, Black-bone silky fowloligopeptides, Ferrous Gluconate.
Methods The animal model of Aplastic anaemia in mice was established by cyclophosphamide method. Mice were randomly divided into control group, model group, AS water extract group and DGWJ granulehigh, medium and low dose groups according to body weight. The blood routine, thymus and spleen indexes of mice were measured by pharmacodynamics, and the intestinal flora of mice was identified by 16 S rDNA sequencing technology. Pseudo-sterile mice were prepared by gavage with compound antibiotics, and the bacterial liquid was prepared from the feces of mice in the treatment group with Aplastic anaemia with obviousearly curative effect. The pseudo-sterile mice were given with bacterial liquid, then CTX was injected intraperitoneally last week to establish a Aplastic anaemia model. The blood routine of mice was measured by pharmacodynamics, and the contents of mice cecum were analyzed by 16 S rDNA sequencing.
Results AS and DGWJ granule can significantly improve the blood routine indexes and anemia symptomscaused by Aplastic anemia model; According to the data of intestinal flora, at the level of genus, Kruskal-Wallis rank sum test was used to evaluate the significance of species richness differences among the four groups,among which Candidatus_Saccharimonas, Lachnospiraceae, Enterorhabdus, Alloprevotella and Turicibacterhad significant differences. The data of fecal microbiota transplantation showed that the low dose group of FMT DGWJ Granule improved the changes of blood routine indexes caused by Aplastic anemia. At the level of genus, the Kruskal-Wallis rank sum test showed that there were significant differences among the four groupssuch as Lactobacillus, Clostridia, Lachnospiraceaeand Colidextribacter. Correlation thermography analysis shows that Lactobacillus had a negative correlation with WBC, RBC, HGB and HCT. unclassified_f__Lachnospiraceae, norank_f__Ruminococcaceae, norank_f__Oscillospiraceae, GCA-900066575, Colidextribacter, Blautia are positively correlated with RBC,HGB and HCT.
Conclusion AS water extract and DGWJ have therapeutic effects on Aplastic anaemia model mice caused by cyclophosphamide, which can improve blood routine, thymus index and spleen index of Aplastic anaemia mice, and are verified by fecal microbiota transplantation experiment. The mechanism of AS water extract and DGWJ in treating Aplastic anaemia may be through increasing the relative abundance of Bacteroides such as Bacteroidota, Patescibacteria, Muribaculacea, Alistipes and Alloprevotella in the intestine of mice with Aplastic anaemia.