bluuebunny/crossref_metadata_2025_split huggingface dataset:
Work–family conflict and mental disorders in the United States: Cross‐sectional findings from The National Comorbidity Survey
Authors: Wang JianLi , Afifi Tracie O. , Cox Brian , Sareen Jitender 
Published: January 2007

AbstractBackgroundWork–family conflict (WFC) may have negative effects on workers' health and productivity. The objective of this analysis was to assess the association between WFC and mental disorders that occurred in the past month.MethodsData from the U.S. National Comorbidity Survey were used. The 1‐month prevalence of mental disorders was estimated by levels of WFC and by gender.ResultsCompared to participants who reported low WFC, those who reported high WFC had a significantly higher prevalence of mental and/or substance use related disorders in the past month. Working hours and domestic roles did not have significant impacts on the association between WFC and mental disorders, irrespective of gender.ConclusionsWork and family roles and the balance between the two are important for workers' mental health. The influence of WFC on mental health should be investigated in conjunction with important work environment characteristics in longitudinal studies. Am. J. Ind. Med. 50:143–149, 2007. © 2007 Wiley‐Liss, Inc.
HPLC study of chlorin e6 and its molecular complex with polyvinylpyrrolidone
Authors: Isakau H. A. , Trukhacheva T. V. , Zhebentyaev A. I. , Petrov P. T. 
Published: January 2007

AbstractSeveral HPLC methods with UV detection were developed for qualitative and quantitative analysis of chlorin e6 and photosensitizer Photolon either in the free form or upon pre‐derivatization (methylation) under reversed‐ and normal‐phase conditions. Optimum analysis conditions providing the best resolution of analytes were found at acidic pH where polar groups are completely protonated. The separation was performed by gradient elution with mobile phases of 0.08% trifluoroacetic acid and acetonitrile on an XTerra RP18 column. The method was specific, accurate and precise, allowing the analysis of chlorin e6 in the presence of numerous degradation products useful in the manufacturing process and quality control of chlorin e6 and Photolon. Copyright © 2007 John Wiley & Sons, Ltd.
A pharmaco‐metabonomic study on the therapeutic basis and metabolic effects of Epimedium brevicornum Maxim. on hydrocortisone‐induced rat using UPLC‐MS
Authors: Li Famei , Lu Xiumei , Liu Huiping , Liu Man , Xiong Zhili 
Published: January 2007

AbstractThis paper describes the pharmaco‐metabonomic study on Epimedium brevicornum Maxim. treated rats with a pathologic condition similar to the ‘kidney deficiency syndromes’ in traditional Chinese medicine and its therapeutic basis. UPLC‐MS technique was used for the development of chemical profile of Epimedium brevicornum Maxim. and endogenous metabolite profiles of rats pre‐ and post‐hydrocortisone interfered and treated with this herbal medicine. The comparison among profiles was performed with a statistical technique, principle component analysis (PCA). Significant difference in endogenous metabolite profiles was observed in the intervention rats and the abnormality of metabolism recovered towards the normal level after administration with Epimedium brevicornum Maxim. extract. Four active constituents of Epimedium brevicornum Maxim. were found into the blood circulation of kidney‐deficient rats and two of their metabolites in the urine. This work suggests that the metabonomic approach is a potentially powerful tool to explore the therapeutic basis and to clarify the possible action mechanism of traditional Chinese medicine. Copyright © 2007 John Wiley & Sons, Ltd.
Port‐a‐Cath perforation of the right atrium closed with an amplatzer ASD occluder
Authors: Wani Sunil , Dick Florian , Meier Bernhard 
Published: January 2007

AbstractRight atrial perforation can lead to tamponade and death. Closure devices are used for sealing of shunts in the heart. We describe an indwelling catheter that caused perforation of the right atrium and was treated with a percutaneous closure device. © 2007 Wiley‐Liss, Inc.
A Facile Synthetic Route to 2 H‐Chromenes: Reconsideration of the Mechanism of the DBU‐Catalyzed Reaction between Salicylic Aldehydes and Ethyl 2‐Methylbuta‐2,3‐dienoate
Authors: Dai Lun‐Zhi , Shi Yong‐Ling , Zhao Gui‐Ling , Shi Min 
Published: January 2007

AbstractReactions of salicylaldehydes with ethyl buta‐2,3‐dienoate or penta‐3,4‐dien‐2‐one catalyzed by a catalytic amount of potassium carbonate produce the corresponding 2 H‐chromene products in moderate to good yields under mild conditions. A plausible reaction mechanism is discussed in the light of the results of an 18O‐labeling experiment. In addition, in view of these findings, the catalytic function of DBU in reactions of this kind is reconsidered.
Fullerene‐Derivatized Amino Acids: Synthesis, Characterization, Antioxidant Properties, and Solid‐Phase Peptide Synthesis
Authors: Yang Jianzhong , Alemany Lawrence B. , Driver Jonathan , Hartgerink Jeffrey D. , Barron Andrew R. 
Published: January 2007

AbstractA series of [60]fullerene‐substituted phenylalanine (Baa) and lysine derivatives have been prepared by the condensation of 1,2‐(4′‐oxocyclohexano)fullerene with the appropriately protected (4‐amino)phenylalanine and lysine, respectively. Conversion of the imine to the corresponding amine is achieved by di‐acid catalyzed hydroboration. The reduction of the imine is not accompanied by hydroboration of the fullerene cage. The [70]fullerene phenylalanine derivative has also been prepared as have the di‐amino acid derivatives. The compounds were characterized by MALDI‐TOF mass spectrometry, UV/Vis spectroscopy, and cyclic voltammetry. 1H and 13C NMR spectroscopy allowed the observation of diastereomers. Fullerene‐substituted peptides may be synthesized on relatively large scale by solid‐phase peptide synthesis. The presence of the C60‐substituted amino acid in a peptide has a significant effect on the secondary structures and self‐assembly properties of peptides as compared to the native peptide. The antioxidant assay of Baa and a Baa‐derived anionic peptide was determined to be significantly more potent than Trolox.
New Structural Features in Heavy Alkaline Earth‐Metal Chemistry — Molecular Heterobimetallic Group 1, Ba Complexes
Authors: Zuniga Maria Felisa , Deacon Glen B. , Ruhlandt‐Senge Karin 
Published: January 2007

AbstractThe preparation of heterobimetallic complexes consisting of alkali and heavy alkaline earth metals remains a challenge due to limited available synthetic strategies. Here we present a new class of group 1, Ba compounds of the type [Mn{Ba(Odpp)2+n}] (M=Na(n=1) (1), K(n=1) (2), Cs(n=1) (3), Li(n=2) (4); HOdpp=2,6‐diphenylphenol) and the Lewis base adducts [Li2(thf)2{Ba(Odpp)4}]⋅PhMe (5) and [K{Ba(Odpp)3(diglyme)}] (6) (diglyme=[bis(2‐methoxy)ethyl ether]) as the first representatives of heterobimetallic group 1, Ba species of low nuclearity. The compounds display a significant degree of metal–arene interaction, believed to be a key factor in stabilizing these highly reactive species. Obtained by solid‐state direct metalation, the target compounds are available without further work‐up.
Expression of nebulette during early cardiac development
Authors: Esham Michael , Bryan Kourtney , Milnes Jennifer , Holmes William B. , Moncman Carole L. 
Published: January 2007

AbstractNebulette, a cardiac homologue of nebulin, colocalizes with α‐actinin in the pre‐myofibrils of spreading cardiomyocytes and has been hypothesized to play a critical role in the formation of the thin‐filament‐Z‐line complex early during myofibrillogenesis. Data from mesodermal explants or whole tissue mounts of developing hearts suggest that the pattern of myofibrillogenesis in situ may differ from observations of spreading cardiomyocytes. To evaluate the role of nebulette in myofibrillogenesis, we have analyzed the expression of nebulette in chicken heart rudiments by immunoblots and immunofluorescence. We detect the 110 kDa nebulette in heart rudiments derived from stage 9–10 using the anti‐nebulin mAb, N114, or polyclonal anti‐nebulette Abs by immunoblotting. Immunofluorescence analysis of explants stained with anti‐nebulette and anti‐α‐actinin Abs demonstrates that both proteins localize along actin filaments in punctate to continuous manner at early stages of cardiac development and later give rise to striations. In both cases, the punctate staining had a periodicity of ∼1.0 μm indicating a pre‐myofibrils distribution at the earliest time points examined. We demonstrate that nebulette is indeed associated with premyofibrils in very early stages of myofibrillogenesis and suggest that nebulette may play an important role in the formation of these structures. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.
Morphological and molecular left–right asymmetries in the development of the proepicardium: A comparative analysis on mouse and chick embryos
Authors: Schulte Inga , Schlueter Jan , Abu‐Issa Radwan , Brand Thomas , Männer Jörg 
Published: January 2007

AbstractThe proepicardium (PE) is an embryonic progenitor cell population that delivers the epicardium, the majority of the cardiac interstitium, and the coronary vasculature. In the present study, we compared PE development in mouse and chick embryos. In the mouse, a left and a right PE anlage appear simultaneously, which subsequently merge at the embryonic midline to form a single PE. In chick embryos, the right PE anlage appears earlier than the left and only the right anlage acquires the full PE‐phenotype. The left anlage remains in a rudimentary state. The expression patterns of PE marker genes (Tbx18, Wt1) correspond to the morphological data, being bilateral in the mouse and unilateral in the chick. Bmp4, which is unilaterally expressed in the right PE of chick embryos, is symmetrically expressed in the sinus venosus wall cranial to the PE in mouse embryos. Asymmetric development of the chicken PE might reflect side‐specific differences in topographical relationships to tissues with PE‐inducing or repressing activity or might result from the PE‐repressing activity of the right PE, which grows earlier. To test these hypotheses, we analyzed PE development in chick embryos, firstly, subsequent to experimentally induced inversion of PE topographical relationships to neighbouring tissues; secondly, in organ cultures; and, thirdly, subsequent to induction of cardia bifida. In all three experiments, only the right PE develops the full PE phenotype. Our results suggest that PE development might be controlled by the L–R pathway in the chick but not in the mouse embryo. Developmental Dynamics 236:684–695, 2007. © 2007 Wiley‐Liss, Inc.
Involvement of TBK1 and IKKϵ in lipopolysaccharide‐induced activation of the interferon response in primary human macrophages
Authors: Solis Mayra , Romieu‐Mourez Raphaëlle , Goubau Delphine , Grandvaux Nathalie , Mesplede Thibault , Julkunen Ilkka , Nardin Alessandra , Salcedo Margarita , Hiscott John 
Published: January 2007

AbstractInterferon (IFN) is an important effector of the innate immune response, induced by different viral or bacterial components through Toll‐like receptor‐dependent and ‐independent mechanisms. In human macrophages and macrophage‐activated killer cells, we demonstrate that (i) the type I IFN response to lipopolysaccharide (LPS) is weak compared to the host response to virus infection; (ii) there is a temporal difference in the induction of tank‐binding kinase‐1 (TBK1) and IkappaB kinase (IKK)‐related kinase epsilon (IKKϵ) kinase activities in response to LPS, with TBK1 activated early and IKKϵ induced in the late phase of IFN induction; and (iii) interferon regulatory factor (IRF)‐7 is induced following LPS treatment, but there is no evidence that IRF‐7 becomes activated by phosphorylation in vivo. Specifically, TBK1 kinase activity is rapidly increased after LPS stimulation (15 min) whereas IKKϵ activation occurs at 8 h. RNA interference‐mediated inhibition of TBK1 and IKKϵ expression in macrophages interfere with IFNB and IRF7 gene expression following LPS activation. Macrophage priming with rIFN‐α increased IRF‐7 expression, led to a sharp up‐regulation of the IFNB gene and to a rapid induction of IFNA2 upon LPS stimulation. These data support a differential role of TBK1 and IKKϵ in the downstream response mediated by IRF‐3 and IRF‐7 to LPS in primary human macrophages.
Translation efficiency of EBNA1 encoded by lymphocryptoviruses influences endogenous presentation of CD8+ T cell epitopes
Authors: Tellam Judy , Rist Michael , Connolly Geoff , Webb Natasha , Fazou Chrysa , Wang Fred , Khanna Rajiv 
Published: January 2007

AbstractLymphocryptoviruses (LCV) that infect humans and Old World primates display a significant degree of genetic identity. These viruses use B lymphocytes as primary host cells to establish a long‐term latent infection and express highly homologous latent viral proteins. Of particular interest is the expression of the EBV‐encoded nuclear antigen‐1 (EBNA1), which plays a crucial role in maintaining the viral genome in B cells. Using human and Old World primate homologues of EBNA1, we show that the internal repeat sequences differentially influence their in vitro translation efficiency. Although the glycine‐alanine repeat domain of human LCV (EBV) EBNA1 inhibits its self‐synthesis, the repeat domains within the simian LCV homologues of EBNA1 do not inhibit self‐synthesis. As a consequence, simian LCV EBNA1‐expressing cells are more efficiently recognized by virus‐specific CTL when compared to human EBV EBNA1, even though both proteins are highly stable in B cells. Interestingly, we also show that similar to human EBNA1, CD8+ T cell epitopes from simian LCV EBNA1 are predominantly derived from newly synthesized protein rather than the long‐lived pool of stable protein. These observations provide additional evidence that supports the theory that immune recognition of EBNA1 can occur without compromising the biological maintenance function of this protein.
Type II collagen without adjuvant induces eosinophilic arthritis
Authors: Bockermann Robert , Holmdahl Rikard 
Published: January 2007

AbstractEosinophilia is a characteristic feature of many inflammatory diseases including inflammatory bowel disease and asthma. It also occurs in a subtype of rheumatoid arthritis but the role of eosinophils has been unclear and animal models have been lacking. Here, we introduce a new mouse model to study the role of eosinophilia in arthritis. Intraperitoneal injection of type II collagen alone, without any adjuvant, was sufficient to induce chronic arthritis in a mouse with transgenic T cells specific for type II collagen. The arthritis was accompanied by infiltration of eosinophils into the synovial tissue and the disease could be blocked with neutralizing anti‐IL‐5 antibodies. To our knowledge, this is the first description of an eosinophilic disease form of destructive arthritis.
Role of the phosphoinositide 3‐kinase p110δ in generation of type 2 cytokine responses and allergic airway inflammation
Authors: Nashed Baher F. , Zhang Tingting , Al‐Alwan Monther , Srinivasan Ganesh , Halayko Andrew J. , Okkenhaug Klaus , Vanhaesebroeck Bart , HayGlass Kent T. , Marshall Aaron J. 
Published: January 2007

AbstractPhosphoinositide 3‐kinases (PI3K) regulate immune activation via their roles in signal transduction of multiple classes of receptors. Here, we examined the effect of genetic inactivation of the hemopoietic cell‐restricted PI3K isoform p110δ on systemic cytokine and chemokine responses and allergic airway inflammation. We found that type 2 cytokine responses (IL‐4, IL‐5 and IL‐13) are significantly decreased in p110δ mutants, whereas type 1 cytokine responses (IFN‐γ and CXCL10) were robust. Elevated IFN‐γ production during the primary response to ovalbumin (OVA) was associated with reduced production of the regulatory cytokine IL‐10. IFN‐γ and IL‐10 production normalized after secondary OVA immunization; however, type 2 cytokine production was persistently reduced. Type 2 cytokine‐dependent airway inflammation elicited by intranasal challenge with OVA was dramatically reduced, with reduced levels of eosinophil recruitment and mucus production observed in the lungs. Induction of respiratory hyper‐responsiveness to inhaled methacholine, a hallmark of asthma, was markedly attenuated in p110δ‐inactivated mice. Adoptive transfer of OVA‐primed splenocytes from normal but not p110δ‐inactivated mice could induce airway eosinophilia in naive, airway‐challenged recipient mice. These data demonstrate a novel functional role for p110δ signaling in induction of type 2 responses in vivo and may offer a new therapeutic target for Th2‐mediated airway disease.
Quantifying the reduction in accessibility of the inhibitory NK cell receptor Ly49A caused by binding MHC class I proteins in cis
Authors: Andersson Katja E. , Williams Geoffrey S. , Davis Daniel M. , Höglund Petter 
Published: January 2007

AbstractMurine natural killer (NK) cells are inhibited by target cell MHC class I molecules via Ly49 receptors. However, Ly49 receptors can be made inaccessible to target cell MHC class I by a cis interaction with its MHC class I ligand within the NK cell membrane. It has recently been demonstrated that MHC class I proteins transfer from the target cells to the NK cell. Here, we establish that the number of transferred MHC class I proteins is proportional to the number of Ly49A receptors at the NK cell surface. Ly49A+ NK cells from mice expressing the Ly49A ligand H‐2Dd showed a 90% reduction in Ly49A accessibility compared to Ly49A+ NK cells from H‐2Dd‐negative mice. The reduction was caused both by lower expression of Ly49A and interactions in cis between Ly49A and H‐2Dd at the NK cell surface. Approximately 75% of the Ly49A receptors on H‐2Dd‐expressing NK cells were occupied in cis with endogenous H‐2Dd and only 25% were free to interact with H‐2Dd molecules in trans. Thus, H‐2Dd ligands control Ly49A receptor accessibility through interactions both in cis and in trans.
Sensory evaluation of the odour of a sunflower oil emulsion throughout oxidation
Authors: Villière Angélique , Rousseau Florence , Brossard Chantal , Genot Claude 
Published: January 2007

AbstractOxidation of unsaturated fatty acids is responsible for off‐flavours, often described as rancid flavours. This study was aimed at describing the evolution of the odour of a sunflower oil‐in‐water emulsion during oxidation at 50  °C in the dark. Appearance of an odour and occurrence of oxidation were first tested for at short‐time aging (0–4 h) by the triangle test and measurements of oxygen consumption, respectively. The odour of the emulsion oxidised for up to 240 h was then characterised by sensory profile, while volatiles issued from lipid oxidation were analysed in the headspace by SPME. From 1 h of aging, while oxygen consumption remained weak, a significant change of the odour was detected by the panel. Up to 21 volatile compounds were identified in the headspace of long‐time‐oxidised emulsions. Beyond the rancid attribute, seven attributes were chosen to describe the odour of oxidised emulsions, four of which referring to solutions of a single volatile oxidation compound. The contribution of the fresh oil attribute, initially dominant, was progressively overtaken from 6 to 24 h of aging by the deep‐fried attribute, which later declined in favour of the painty attribute, predominant after 100 h. Evolution of intensity scores and contribution to odour profiles of attributes could not be easily related to one reference volatile compound or another, confirming the complex relationship between the generated volatile compounds and the perceived odour.
Nonpeptide Integrin Antagonists: RGD Mimetics Incorporating Substituted Azabicycloalkanes as Amino Acid Replacements
Authors: Manzoni Leonardo , Bassanini Michele , Belvisi Laura , Motto Ilaria , Scolastico Carlo , Castorina Massimo , Pisano Claudio 
Published: January 2007

AbstractAzabicyclo[4.3.0]alkanes appropriately substituted on both cycles have been synthesized as potential scaffold mimics of the RGD signaling motif of integrin. Two sets of functionalized azabicycloalkanes have been examined. In vitro assays established that21has a good affinity specifically for αvβ3. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Aging and brain activation with working memory tasks: an fMRI study of connectivity
Authors: Cook Ian A. , Bookheimer Susan Y. , Mickes Laura , Leuchter Andrew F. , Kumar Anand 
Published: January 2007

AbstractBackgroundWhite matter changes in aging and neuropsychiatric disorders may produce disconnection of neural circuits. Temporal correlations in regional blood oxygen level dependent (BOLD) signals may be used to assess effective functional connectivity in specific circuits, such as prefrontal cortex (PFC) circuits supporting working memory (WM) tasks. We hypothesized healthy older subjects would show lower connectivity than younger subjects.MethodsHealthy younger (n = 9, 25.9 (SD 6.0) years) and older adults (n = 11, 68.3 (4.9) years) performed WM tasks during functional MRI. Subjects viewed images and were instructed to label them, either simultaneously or after a delay; BOLD responses with and without delay were contrasted to assess differential WM activation and connectivity. Two tasks were used: a semantic task, with line drawings categorized as ‘alive’ or ‘not living’, and an emotional task, with emotive faces as stimuli and subjects selecting the better emotional description.ResultsIn both tasks, older subjects activated larger regions and had greater inter‐individual variability in extent of activation. In the semantic task, connectivity was lower in the older subjects for the amygdala/orbital PFC circuit (p = 0.04). Contrary to our predictions, older subjects exhibited higher connectivity than younger subjects in the circuit linking orbital and dorsolateral PFC in both semantic (p = 0.04) and emotional (p = 0.02) tasks.ConclusionsHealthy subjects exhibited age‐dependent differences in connectivity in working memory circuits, but this may reflect effects of aging on white matter, compensatory mechanisms, and other factors. Volumetric determination of white matter hyperintensities in future studies may clarify the functional importance of structural damage. Copyright © 2007 John Wiley & Sons, Ltd.
Depression and the risk for cardiovascular diseases: systematic review and meta analysis
Authors: Van der Kooy Koen , van Hout Hein , Marwijk Harm , Marten Haan , Stehouwer Coen , Beekman Aartjan 
Published: January 2007

AbstractBackgroundDepression and cardiovascular diseases are both common among elderly. Depression is suspected to be an independent risk factor for the onset of coronary heart disease, yet it is not clear to what extent and if depression also is associated with the onset of other diseases of the circulatory system.AimsTo estimate the risk of depression as an independent risk factor for various cardiovascular diseases (CVD) and explore the effects of heterogeneity and methodological quality.MethodMeta‐analyses and meta‐regression analyses of longitudinal cohort and case‐control studies reporting depression at baseline and CVD outcomes at follow‐up.Data sourcesMEDLINE (1966–2005) and PSYCHINFO (1966–2005).ResultsOf the 28 studies that met the inclusion criteria, 11 were assesed as high quality studies. Although depressed mood increased the risk for a wide range of CVDs, heterogeneity was substantial in most cases. Only the overall combined risk of depression for the onset of myocardial infarctions (n=8, OR=1.60, 95%CI 1.34–1.92) was homogenous. Clinically diagnosed major depressive disorder was identified as the most important risk factor for developing CVD.ConclusionsDepression seems to be an independent risk factor for the onset of a wide range of CVDs, although this evidence is related to a high level of heterogeneity. Copyright © 2007 John Wiley & Sons, Ltd.