bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential effects of cholecalciferol and calcitriol on muscle proteolysis and oxidative stress in angiotensin II‐induced C2C12 myotube atrophy
Authors: Hirunsai Muthita , Srikuea Ratchakrit 
Published: April 2024

AbstractRenin–angiotensin system activation contributes to skeletal muscle atrophy in aging individuals with chronic diseases. We aimed to explore the effects of cholecalciferol (VD3) and calcitriol (1,25VD3) on signaling of muscle proteolysis and oxidative stress in myotubes challenged with angiotensin II (AII). The mouse C2C12 myotubes were assigned to vehicle, AII, AII + VD3, AII + 1,25VD3, and AII + losartan groups. The expression levels of muscle‐specific E3 ubiquitin ligase proteins, autophagy‐related proteins, and oxidative stress markers were investigated. We demonstrated the diverse effects of VD3 and 1,25VD3 on AII‐induced myotube atrophy. The myotube diameter was preserved by treatment with 100 nM VD3 and losartan, while 1 and 10 nM 1,25VD3 increased levels of FoxO3a, MuRF1, and atrogin‐1 protein expression in myotubes exposed to AII. Treatment with AII + 10 nM 1,25VD3 resulted in the upregulation of LC3B‐II, LC3B‐II/LC3B‐I, and mature cathepsin L, which are autophagic marker proteins. The p62/SQSTM1 protein was downregulated and vitamin D receptor was upregulated after treatment with AII + 10 nM 1,25VD3. A cellular redox imbalance was observed as AII + 10 nM 1,25VD3‐induced reactive oxygen species and NADPH oxidase‐2 overproduction, and these changes were associated with an inadequate response of antioxidant superoxide dismutase‐1 and catalase proteins. Collectively, these findings provide a translational perspective on the role of vitamin D3 in alleviating muscle atrophy related to high levels of AII.
Preparation and pharmacodynamic evaluation of sodium aescinate solid lipid nanoparticles
Authors: Jiang Xiaohong , Shen Zhongfei , Shen Bin , Sun Ying 
Published: April 2024

Abstract
Recent advancements in nanotechnology have spotlighted lipid nanocarriers as potent mediums for drug delivery, with solid lipid nanocarriers (SLNs) emerging as a key focus due to their unique structural attributes. This research specifically addresses the development and evaluation of the anti-inflammatory properties of SLNs loaded with sodium aescinate. To identify the most effective composition, a detailed pseudo-ternary phase diagram was employed. The production process of these SLNs involved sophisticated high-pressure homogenization techniques. For characterization, the average particle size and zeta potential were precisely measured using advanced laser diffractometry. Additionally, to ascertain the effectiveness of the drug encapsulation, the SLNs underwent a rigorous high-speed centrifugation process, enabling accurate determination of both the encapsulation efficiency and drug-loading capacity. The results of this research reveal that a relatively refined method for determining sodium aescinate content has been established, and a reasonable formulation has been selected for the preparation of sodium aescinate solid lipid nanoparticles. The average particle size was 142.32 ± 0.17 nm, the zeta potential was 1.60 ± 0.32 mV, and the encapsulation rate was 73.93 ± 4.65%. The drug loading was 13.41 ± 1.25%. In conclusion, this method can produce stable solid lipid nanoparticles containing sodium aescinate with uniform particle size, even distribution after encapsulation, and significant anti-inflammatory activity.
Fano varieties with torsion in the third cohomology group
Authors: Ottem John Christian , Rennemo Jørgen Vold , Kollár János 
Published: April 2024

AbstractWe construct first examples of Fano varieties with torsion in their third cohomology group. The examples are constructed as double covers of linear sections of rank loci of symmetric matrices, and can be seen as higher-dimensional analogues of the Artin–Mumford threefold. As an application, we answer a question of Voisin on the coniveau and strong coniveau filtrations of rationally connected varieties.
Language ideologies and speaker categorization: a case study from the U.S. legal system
Authors: Dundon John Terry 
Published: April 2024

Abstract
This study examines a judicial opinion from an appellate court in the District of Columbia, in the United States, using membership categorization analysis. The appeal concerned the absence of an interpreter during the police interrogation of a person suspected of having committed a crime, and whether this absence violated a local law about the provision of interpreters. Deciding this appeal required the court to determine whether the defendant had met a statutory definition of “communication-impaired” persons who are entitled to interpretation services. I argue that, in determining whether the defendant fit into this legal category, the court discursively constructed two linguistic categories that helped support its ultimate disposition of the appeal. These linguistic categories were hierarchically positioned, with English speakers as a default and non-English speakers as somehow deficient or unable to fully function in society. The court’s opinion also contemplated a binary choice of a person being able to communicate in English fully, or not at all, with the possibility that a person might be proficient in English for some purposes, but not others, often presented as a concession or ancillary point. Taken as a whole, the category construction in the opinion suggests an ideology of English monolingualism, which belies a reality of multilingualism, code-shifting, and mixed linguistic identities.
A very rare presentation of mitochondrial elongation factor Tu deficiency-TUFM mutation and literature review
Authors: Gokalp Sabire , Inci Asli , Kilic Ayse , Ozsaydi Ekin , Altun Ayse Nur , Demir Fevzi , Ergin Filiz Basak , Ozbek Mehmet Nuri , Okur Ilyas , Ezgu Fatih , Tumer Leyla 
Published: April 2024

Abstract

Objectives
The mitochondrial elongation factor Tu (EF-Tu), encoded by the TUFM gene, is a GTPase, which is part of the mitochondrial protein translation mechanism. If it is activated, it delivers the aminoacyl-tRNAs to the mitochondrial ribosome. Here, a patient was described with a homozygous missense variant in the TUFM [c.1016G>A (p.Arg339Gln)] gene. To date, only six patients have been reported with bi-allelic pathogenic variants in TUFM, leading to combined oxidative phosphorylation deficiency 4 (COXPD4) characterized by severe early-onset lactic acidosis, encephalopathy, and cardiomyopathy.

Case presentation
The patient presented here had the phenotypic features of TUFM-related disease, lactic acidosis, hypotonia, liver dysfunction, optic atrophy, and mild encephalopathy

Conclusions
We aimed to expand the clinical spectrum of pathogenic variants of TUFM.
On the stability analysis of a restrained functionally graded nanobeam in an elastic matrix with neutral axis effects
Authors: Civalek Ömer , Uzun Büşra , Yaylı Mustafa Özgür 
Published: April 2024

Abstract
In this work, a general eigenvalue solution of an arbitrarily constrained nonlocal strain gradient nanobeam made of functionally graded material is presented for the first time for the stability response by the effect of the Winkler foundation. Elastic springs at the ends of the nanobeam are considered in the formulation, which have not been considered in most studies. In order to analyze deformable boundary conditions, linear equation systems are derived in terms of infinite power series by using the Fourier sine series together with the Stokes’ transform. The higher-order force boundary conditions are used to obtain a coefficient matrix including different end conditions, power-law index, elastic medium, and small-scale parameters. A general eigenvalue problem of technical interest, associated with nonlocal strain gradient theory, is mathematically evaluated and presented in detail. Parametric results are obtained to investigate the effects of material length scale parameter, Winkler stiffness, power-law index, nonlocal parameter, and elastic springs at the ends. In addition, the effects of the other higher-order elasticity theories simplified from nonlocal strain gradient theory are also investigated and some benchmark results are presented.
Osteotomías supramaleolares en pacientes con artrosis de tobillo: resultados clínicos y radiográficos
Authors: Conti Leonardo Ángel , Verbner Jonathan M. , Valenti Pablo , Parise Ana Cecilia , Villena Daniel Sebastián , Santini Araujo María Gala , Sotelano Pablo , Cardone Guillermo , Bilbao Facundo , Carrasco N. Marina 
Published: April 2024

Introducción: Las osteotomías supramaleolares son una opción terapéutica para la artrosis asimétrica de tobillo. El objetivo de este artículo es describir los resultados clínicos, radiográficos y subjetivos obtenidos en pacientes con artrosis de tobillo sometidos a una osteotomía supramaleolar.
Materiales y Métodos: Estudio observacional descriptivo retrospectivo de pacientes sometidos a una osteotomía supramaleolar por artrosis de tobillo entre enero de 2010 y julio de 2017. Se analizaron las historias clínicas para recabar datos clínicos y radiográficos preoperatorios y posoperatorios.
Resultados: Se incluyó a 13 pacientes: 8 con una desalineación del tobillo y retropié en valgo (61,5%) y 5 (38,5%) en varo. El puntaje medio posoperatorio de la escala analógica visual del grupo con deformidad en varo disminuyó de 9 ± 0,45 a 3 ± 1 (p <0,05) y de una media de 7,88 ± 0,35 a 2,15 ± 1,64 (p <0,05) en aquellos con deformidad en valgo. El puntaje medio de la escala de la AOFAS se modificó de 32,8 ± 16,2 antes de la cirugía a 82,1 ± 13,6 en el posoperatorio, en el grupo con deformidad en varo (p <0,05) y de 31 ± 17,3 a 93,1 ± 6,20, respectivamente, en aquellos con deformidad en valgo (p <0,05). Se constató la consolidación radiográfica en 12 pacientes (92,3%), en un promedio de 10.4 semanas.
Conclusión: La osteotomía supramaleolar como tratamiento de la artrosis asimétrica de tobillo es una técnica predecible, con muy buenos resultados clínicos y radiográficos a corto y mediano plazo.
Asistencia artroscópica y fijación interna percutánea en fracturas de Maisonneuve. Evaluación por imágenes de los resultados
Authors: Herrera Horacio , Rofrano Martín , Azulay Guillermo , Andrés Santiago , Varaona José María , Pereira Francisco , Tálamo Francisco 
Published: April 2024

Introducción: La fractura de Maisonneuve es una lesión caracterizada por la fractura subcapital del peroné asociada a una lesión capsuloligamentaria de tobillo. Su tratamiento supone la restauración de la anatomía ósea y capsuloligamentaria normal para restablecer las fuerzas de contacto tibioastragalinas fisiológicas. Esta calidad de reducción puede ser difícil de alcanzar, sobre todo, con técnicas percutáneas.
Objetivo: Evaluar mediante imágenes la calidad de la reducción de fracturas de Maisonneuve reducidas en forma cerrada (bajo visualización directa artroscópica) y fijadas por vía percutánea.
Materiales y Métodos: Se analizaron radiografías e imágenes de tomografía computarizada preoperatorias y posoperatorias, comparativas de los tobillos operado y sano.
Resultados: Se evaluaron 13 fracturas. Los parámetros radiográficos posoperatorios (espacio claro medial, solapamiento tibioperoneo distal, espacio claro tibioperoneo) no registraron diferencias, excepto por el intervalo tibioperoneo anterior que aumentó en un caso. La medición tomográfica posoperatoria del solapamiento tibioperoneo distal y el intervalo tibioperoneo anterior reveló que todas las reducciones eran satisfactorias. Dos pacientes tenían valores alterados en la sindesmosis tibioperonea distal con diferencia >2 mm respecto del tobillo sano.
Conclusión: Recomendamos la asistencia artroscópica para el manejo de lafractura de Maisonneuve como herramienta de control intraoperatorio para una fijación percutánea más segura.
Biocontrol de Penicillium digitatum con metabolitos secundarios de hongos endófitos en frutos de mandarina
Authors: León-Ttacca Betsabe , Arestegui-Cantoral Yasmin , Tarula-Gutierrez Brandy , Orellana-Cornejo Cesar , Luis-Vilcamiza Jorge , Flores-Pelaes Pedro 
Published: April 2024

Introducción. El “moho verde” causado por Penicillium digitatum es uno de los problemas fitosanitarios que limita la producción en los cítricos. Para su control se utilizan fungicidas sintéticos, que con el tiempo han perdido eficacia debido a la resistencia generada por el hongo. Una alternativa, al control químico, el uso de hongos endófitos con acción biocontroladora. Objetivo. Evaluar el efecto de metabolitos secundarios (MS) de hongos endófitos (HE) en la inhibición micelial de Penicillium digitatum y la eficacia de control de la enfermedad, en frutos de mandarina (Citrus reticulata) después de la cosecha. Materiales y métodos. Se realizó bajo un diseño experimental completo al azar en el laboratorio de Sanidad Vegetal de la Universidad Nacional de Cañete (UNDC), Lima, Perú. Para la prueba de antibiosis se extrajeron los metabolitos secundarios de diez cepas de HE de los géneros Trichoderma sp., Fusarium sp. y Aspergillus sp., en donde se evaluó la inhibición micelial del patógeno (%). Se asperjaron MS a una concentración del 15 % (v/v) sobre frutos de mandarina que fueron desinfectados y luego inoculados por aspersión (1x 105 UFC/cc) con el patógeno. En los tratamientos se adicionaron un biocontrol comercial, un aceite vegetal y un fungicida. Después de seis y nueve días, se evaluaron la incidencia (%) y el índice de severidad (0,1,2,3) de la enfermedad. Los datos fueron analizados con el programa estadístico InfoStat. Resultados. Los metabolitos secundarios de las cepas HEA-111 (Aspergillus), HEA-109 (Fusarium) y HSA-1 (Trichoderma) presentaron la mayor inhibición micelial con 95,05 %, 87,51 % y 47,47 %, respectivamente. En los frutos de mandarina tratados con los MS de las cepas HEA-109 y HSA-1 hubo un control del 80 % y 55 %, respectivamente. Conclusiones. Los MS de HE tienen efecto en la inhibición micelial de de P. digitatum y redujo el avance de la enfermedad en frutos de mandarina después de la cosecha.
Melioidosis: A new emerging infection in West Coastal Maharashtra
Authors: Napa Preetham , Lokapure Sumedh Sudhakar , Kulkarni Ajit , Keni Ajay , Kulkarni Amol , Deshpande Sanjay Ram , Machnurkar Kaustubh , Wasgaonkar Gopal 
Published: April 2024

Melioidosis is a zoonotic infectious disease caused by a gram-negative bacterium . It is an endemic disease in Southeast Asia and Northern Australia, but under reported from India. This study was conducted to determine the geographical epidemiology, risk factors, clinical presentations associated with melioidosis. It is retrospective study of six culture proven cases of Melioidosis from a tertiary care hospital. The six cases of melioidosis were from same geographical location from western coast of Maharashtra. The age group ranged from 3 years to 55 years but more predominant group was within 30-to-50-years. All were males (100%), no female patient was affected. Majority of cases were presented to the hospital in rainy season, from June to September, coincides with season of heavy rainfall. Alcoholism (83.33%) and diabetes mellitus (66.66%) were major risk factors involved. Fever (100%) was most common presenting symptoms. The organ space abscess (50%) involving lymph node, liver and spleen were common followed by disseminated disease and pulmonary melioidosis. All patients were treated with ß-lactam antibiotics as intensive therapy followed by oral cotrimoxazole as eradication therapy. Melioidosis is an emerging infection in India specially in the coastal region with very heavy rainfalls. Male gender is prone to develop the infection with diabetes and alcoholism are additional risk factors. The clinical presentation may vary from localised organ space abscess to septic shock with high mortality. The accurate diagnosis and prompt treatment play key role in managing melioidosis.
Evaluation of Leukocyte esterase and Nitrite dipstick tests with routine urine microscopic analysis in detecting urinary tract infections
Authors: Hans Agam , Yadav Abhishek , Kaur Parneet , Kumari Archana 
Published: April 2024

: Urinary tract infection is a global health problem affecting all age groups. is the most common cause of UTI followed by , staphylococcus haemolyticus and enterococci etc. The gold standard for detecting an UTI is the presence of pathogen in urine along with clinical symptoms and pyuria. Nitrite (NIT) and leukocyte esterase (LE) tests are two important dip stick tests used for screening UTI. A total of 202 patients who presented with clinical symptoms of UTI from January 2023 to December 2023 were evaluated for urine routine and culture examination. LE and NIT dipstick tests were evaluated and change of colour was considered positive. Microscopic examination of urine was performed manually and urine culture with count of &#62; 10 CFU/ml was considered positive. Statistical data was analysed using IBM SPSS v 29.0.2.0 (20) and Microsoft Excel. Sensitivity, specificity, positive predictive value, negative predictive value and accuracy were calculated for both tests.: LE had a sensitivity of 47.22% whereas NIT was much less sensitive (15.27%). Specificity of NIT (99.23%) was higher in comparison to LE (81.53%). NIT had overall better PPV and NPV (91.66%, and 67.89%) as compared to LE (58.62% and 73.61%). The accuracy of LE and NIT were 69.3% and 69.8% respectively. A positive correlation was also seen with increasing WBC count and positive urine culture.: Urine culture along with clinical and routine analysis is necessary for definitive diagnosis of UTI but importance of dipstick chemical examination should not be underestimated. LE and NIT have an additional benefit of quick results in comparison to culture which takes at least 24 hours.
Peripheral blood smear diagnosis of invasive candidiasis in a patient of myelodysplastic syndrome/myeloproliferative neoplasm treated with hypomethylating agent: A case report
Authors: Fokmare Akanksha Sanjay , Parikh Rupali Shripal , Marathe Paresh R , Gupta Siddesh Tribhuvan 
Published: April 2024

Patients with haematological malignancies undergoing treatment with hypomethylating agents are predisposed to opportunistic infections, including Candidiasis. We present the case of a 73-year-old female patient diagnosed with Myelodysplastic/Myeloproliferative neoplasms who was receiving a hypomethylating agent as part of her treatment regimen. During the course of her treatment, peripheral blood smear examination revealed the presence of budding yeast cells, consistent with Candida species. Subsequent culture confirmed the diagnosis of Candidiasis.The development of Candidiasis in patients with haematological malignancies receiving hypomethylating agents poses a significant clinical challenge due to overlapping symptoms with other infections and chemotherapy-induced myelosuppression. Peripheral smear examination serves as a rapid and cost-effective adjunctive diagnostic tool for the early detection of Candidiasis in these patients. Timely detection of Candidiasis in this population is critical for appropriate management and improved outcomes.
Digital governance in transforming health systems and health policy in India
Authors: Kar Manoj , Ram Sipra 
Published: April 2024

The cornerstone of a thriving nation is the health of its citizens, and Universal Health Coverage (UHC) stands as a vital measure of success of its health systems in promoting inclusivity and well-being. The 21st century has seen a growing focus on reshaping health systems around individuals, with integration of data and digital technologies offering significant opportunities for advancing concept of ‘digital governance in transforming health systems’. However, despite the challenges faced by health policymakers, a tangible transformation in 'digital health governance' is still underway.This policy review explores India's digital governance initiatives aimed at revolutionizing healthcare accessibility and delivery to contribute to the realization of UHC. It sheds light on national digital health programs like National Digital Health Blueprint (NDHB), National Digital Health Mission (NDHM), Ayushman Bharat Digital Health Mission, and global health interventions. Addressing challenges such as limited infrastructure and hesitancy toward digital adoption, the paper emphasizes solutions like capacity building and awareness generation as discussed within the national health systems framework. It underscores the importance of optimal governance models fostering interdisciplinary collaboration among key stakeholders, including government, startups, hospitals, and academic institutions. The ultimate goal is to offer insights into strategic digital transformations, supported by robust public policies and governance mechanisms, which can shape a more equitable, efficient, and digitally empowered healthcare ecosystem. This, in turn, contributes to the progress of UHC in India by 2030 (UN-SDGs).
Odpowiedzialność w procesie wychowania
Authors: Rynio Alina 
Published: April 2024

Publikacja traktując odpowiedzialność jako wartość konstytutywną i egzystencjalnie znaczącą przybliża jej aspekt pedagogiczny obejmujący kształtowanie postawy odpowiedzialności dzieci, młodzieży, dorosłych, rodziców, nauczycieli, katechetów czy zarządzających oświatą i wychowaniem. W pierwszej części autorka szuka odpowiedzi na pytanie: dlaczego nie można być dobrym uczniem, dobrym rodzicem, a tym bardziej dobrym obywatelem czy nauczycielem bez dojrzałego podjęcia wychowania i samowychowania do odpowiedzialności? Chcąc dać odpowiedź na tak postawiony problem, analizuje czym w swojej istocie jest uczniowska i nauczycielska odpowiedzialność.
W części drugiej, mając na uwadze to, że uczymy się poprzez przykłady, autorka publikacji przywołuje mało znaną, choć zweryfikowaną życiem wielu osób koncepcję współczesnego wychowania do wolności opartego na formowaniu postawy odpowiedzialności. Jest to koncepcja pozostawiona przez zmarłego w 2005 r. ks. Luigiego Giussaniego – założyciela znanego w całym świecie włoskiego ruchu kościelnego Comunione e Liberazione. W koncepcji tej interesujące wydają się podstawowe wymiary wychowania do odpowiedzialności. Są nimi: wychowanie do uwagi, wychowanie do odważnej akceptacji prawdy, wychowanie do postawy pytającej, wychowanie do ciekawości i pozytywnego odniesienia do rzeczywistości, związek wychowania do odpowiedzialności ze wspólnotą, autorytetem i krytycznie rozumianą tradycją czy spotkanie z ludźmi prawdziwie odpowiedzialnymi jako sposób poszerzania pola własnej odpowiedzialności.
W części finalnej autorka dostrzegając konieczność respektowania odpowiedzialności jako ważnej kategorii ludzkiego życia i celu wychowania integralnego, sygnalizuję kilka kluczowych kwestii związanych ze skutecznym wychowaniem do odpowiedzialności w edukacji XXI wieku.