bluuebunny/crossref_metadata_2025_split huggingface dataset:
The transcriptomic signature of respiratory sensitizers using an alveolar model
Authors: Gibb Matthew , Liu James Y. , Sayes Christie M. 
Published: April 2024

AbstractEnvironmental contaminants are ubiquitous in the air we breathe and can potentially cause adverse immunological outcomes such as respiratory sensitization, a type of immune-driven allergic response in the lungs. Wood dust, latex, pet dander, oils, fragrances, paints, and glues have all been implicated as possible respiratory sensitizers. With the increased incidence of exposure to chemical mixtures and the rapid production of novel materials, it is paramount that testing regimes accounting for sensitization are incorporated into development cycles. However, no validated assay exists that is universally accepted to measure a substance’s respiratory sensitizing potential. The lungs comprise various cell types and regions where sensitization can occur, with the gas-exchange interface being especially important due to implications for overall lung function. As such, an assay that can mimic the alveolar compartment and assess sensitization would be an important advance for inhalation toxicology. Some such models are under development, but in-depth transcriptomic analyses have yet to be reported. Understanding the transcriptome after sensitizer exposure would greatly advance hazard assessment and sustainability. We tested two known sensitizers (i.e., isophorone diisocyanate and ethylenediamine) and two known non-sensitizers (i.e., chlorobenzene and dimethylformamide). RNA sequencing was performed in our in vitro alveolar model, consisting of a 3D co-culture of epithelial, macrophage, and dendritic cells. Sensitizers were readily distinguishable from non-sensitizers by principal component analysis. However, few differentially regulated genes were common across all pair-wise comparisons (i.e., upregulation of genes SOX9, UACA, CCDC88A, FOSL1, KIF20B). While the model utilized in this study can differentiate the sensitizers from the non-sensitizers tested, further studies will be required to robustly identify critical pathways inducing respiratory sensitization.
Graphical Abstract
Graphical headlines/headlights

Pollutants may trigger lung allergies, but no universal method measures respiratory sensitization potential.

In vitro systems can detect respiratory sensitizers, aiding in anticipating and reducing the risks of new materials.

Sensitizers and non-sensitizers can be distinguished through transcriptome investigation.

The sensitizers tested induced cell differentiation and proliferation pathways while inhibiting immune defense and functionality.
Analytical Model for Non-linear $$\user2{M - }\,\,{\varvec{\theta}}$$ Relationships of Dowel-Type Timber Connections Exposed to Fire
Authors: Nakayama Yukito , Kikuchi Takayuki , Totsuka Marina , Hirashima Takeo 
Published: April 2024

AbstractRecent experimental evidence has shown that wood–steel–wood dowel-type connections exhibit a semi-rigid behaviour even after 90 min of fire exposure. Because a semi-rigid behaviour influences the bending moment distribution among structural members, considering the moment–rotation relationships within frame analyses in which structural members are modelled as beam elements can enable a realistic fire response analysis that is significantly simpler than the three-dimensional finite element method. This study proposes an analytical methodology that accounts for the thermo-mechanical behaviour of timber and dowels, enabling the simulation of the non-linear moment–rotation relationships under fire conditions. The proposed analytical model divides dowels into a series of elements on an elastoplastic foundation and performs a direct stiffness method in a time-incremental procedure using an element stiffness matrix derived from beam-on-elastic-foundation theory. This study also presents the results of load-carrying tests on timber frames with dowel-type connections performed under ambient and fire conditions. The analytical results were consistent with the fire test results. Additionally, the analyses were also performed under three conditions in which the dowels were rigid, linear elastic, and elastoplastic bodies. These three results converged to the same value after 65 min of heating, which suggests that the ultimate states of beams with dowel-type connections exposed to fire can be modelled by assuming that dowels are rigid bodies.
Corneal endothelial changes seven years after phacoemulsification cataract surgery
Authors: Lundberg Björn 
Published: April 2024

Abstract
Purpose
To evaluate long-term postoperative corneal changes after phacoemulsification cataract surgery.

Methods
Twenty patients who participated in a previous study regarding corneal endothelial changes after phacoemulsification cataract surgery were examined after 7 years. The patients were divided in three groups based on their initial increase in central corneal thickness day one after the surgery: < 5% increase, 6–20% increase and ≥ 20% increase. The primary outcome measures were corneal endothelial cell loss (ECL), endothelial cell count (ECC) and endothelial morphology.

Results
After 7 years, a difference in cell loss between the groups was observed, except for groups 1 and 2. Endothelial cell count (ECC) differed significantly between groups 1 and 3 at 3 months. At 7 years, there was no difference in ECC between the three groups. Cell loss was found exclusively in group 1 between 3 months and 7 years. Endothelial cell morphology showed a converging pattern between 3 months and 7 years.

Conclusion
After phacoemulsification cataract surgery, long-term ECC and morphology appear to converge towards a comparable steady state regardless of initial corneal swelling and endothelial cell loss.
Novel Synonymous Variant in IL7R Causes Preferential Expression of the Soluble Isoform
Authors: Mackeh Rafah , El Bsat Yasmin , Elmi Asha , Bibawi Hani , Karim Mohammed Yousuf , Hassan Amel , Lo Bernice 
Published: April 2024

Abstract
Purpose
The interleukin-7 receptor (IL-7R) is primarily expressed on lymphoid cells and plays a crucial role in the development, proliferation, and survival of T cells. Autosomal recessive mutations that disrupt IL-7Rα chain expression give rise to a severe combined immunodeficiency (SCID), which is characterized by lymphopenia and a T−B+NK+ phenotype. The objective here was to diagnose two siblings displaying the T−B+NK+ SCID phenotype as initial clinical genetic testing did not detect any variants in known SCID genes.

Methods
Whole genome sequencing (WGS) was utilized to identify potential variants causing the SCID phenotype. Splicing prediction tools were employed to assess the deleterious impact of the mutation. Polymerase Chain Reaction (PCR), Sanger sequencing, flow cytometry, and ELISA were then used to validate the pathogenicity of the detected mutation.

Results
We discovered a novel homozygous synonymous mutation in the IL7R gene. Our functional studies indicate that this variant is pathogenic, causing exon 6, which encodes the transmembrane domain, to be preferentially spliced out.

Conclusion
In this study, we identified a novel rare synonymous mutation causing a loss of IL-7Rα expression at the cellular membrane. This case demonstrates the value of reanalyzing genetic data based on the clinical phenotype and highlights the significance of functional studies in determining the pathogenicity of genetic variants.
Impact of an Oral Health Education Program on the Oral Health Literacy of Refugees
Authors: Muller Romana , Bilich Lisa , Jones Merri 
Published: April 2024

AbstractInadequate comprehension of healthcare information contributes to poor health outcomes. Ethnic minorities are one of the populations most affected by low health and oral health literacy (OHL). The hypothesis of the current study was that an oral health education program (OHEP) can improve the OHL, oral health awareness and behaviors of refugees. We also hypothesized that there will be a difference between OHL in English and native language in the Pre-intervention phase. Fifty-two adult refugees participated in an educational program that included a comprehensive and culturally sensitive PowerPoint presentation and hands-on learning activities on oral health topics. The study used a cross-sectional pre-post study design. Before the program (Pre-intervention group), participants completed 2 surveys: Sociodemographic Survey and Oral Health Perceptions of Refugees in a bilingual format, and the Estimate of Oral Health Literacy-Bilingual40 (EOHL-BL40) in English, and in the participants’ native language. Immediately after an OHEP (Post-intervention group), participants completed the EOHL-BL40 survey in English only. Two weeks after OHEP (Follow-up intervention), participants completed again the Sociodemographic Survey and Oral Health Perceptions of Refugees and the Estimate of Oral Health Literacy-Bilingual40 (EOHL-BL40) in English only. The mean percentage of words understood on Pre-intervention EOHL-BL40 survey were significantly higher in native languages (47.3% ±3.7%) compared with survey in English (15.3% ±1.2%, P < 0.001). Post-intervention scores, immediately after educational program, were higher (28.6% ±2.4%), P < 0.001) for survey in English compared with the Pre-intervention (15.3% ±1.2%). The follow-up (2 weeks later) scores (25.5% ±2.4) were slightly lower than Post-intervention but still significantly higher (P < 0.001) than Pre-intervention. Results for both Sociodemographic Survey and Oral Health Perceptions of Refugees surveys were similar: the OHEP positively affected oral health awareness and oral health behaviors. Results of the study suggested that OHEP may improve oral health literacy, oral health awareness, and personal oral hygiene practices of refugees.
The Prevalence of Cognitive Impairment in Relapsing-Remitting Multiple Sclerosis: A Systematic Review and Meta-analysis
Authors: Wu Wendy , Francis Heather , Lucien Abbie , Wheeler Tyler-Ann , Gandy Milena 
Published: April 2024

AbstractIt is increasingly recognized that cognitive symptoms are a common sequelae of relapsing-remitting multiple sclerosis and are associated with adverse functional consequences. However, estimates of cognitive impairment (CIm) prevalence vary widely. This study aimed to determine the pooled prevalence of CIm among adults with RRMS and investigate moderators of prevalence rates. Following prospective registration (PROSPERO; CRD42021281815), electronic databases (Embase, Scopus, Medline, and PsycINFO) were searched from inception until March 2023. Eligible studies reported the prevalence of CIm among adults with RRMS, as determined through standardized neuropsychological testing and defined as evidence of reduced performance across at least two cognitive domains (e.g., processing speed, attention) relative to normative samples, healthy controls, or premorbid estimates. The electronic database search yielded 8695 unique records, of which 50 met selection criteria. The pooled prevalence of cognitive impairment was 32.5% (95% confidence interval 29.3–36.0%) across 5859 participants. Mean disease duration and age were significant predictors of cognitive impairment prevalence, with samples with longer disease durations and older age reporting higher prevalence rates. Studies which administered more extensive test batteries also reported significantly higher cognitive impairment prevalence. Approximately one third of adults with RRMS experience clinical levels of CIm. This finding supports the use of routine cognitive testing to enable early detection of CIm, and to identify individuals who may benefit from additional cognitive and functional support during treatment planning.
Simultaneous Minimization of Water Age and Pressure in Water Distribution Systems by Pressure Reducing Valves
Authors: Korder Kristina , Cao Hao , Salomons Elad , Ostfeld Avi , Li Pu 
Published: April 2024

AbstractPressure reducing valves (PRVs) are essentially used to reduce operational pressures in water distribution systems (WDSs) to minimize water leakage. However, water age in a WDS is an important variable describing the water quality and should be kept as low as possible. Therefore, the aim of this study is to investigate the possibility and potential of simultaneously minimizing both pressure and water age by using PRVs. To determine the optimal location and setting of PRVs, a mixed-integer nonlinear programming (MINLP) problem is formulated with minimization of the sum of the weighted total water age and pressure as the objective function, where the weighting factor can be defined by the user’s preference. The equality constraints consist of the hydraulic equations and water age functions to describe pressure and water age in the distribution network, while the inequality constraints ensure them in the defined operating ranges, respectively. Applying the proposed approach to two case studies, the results show that both water age and pressure can indeed be significantly reduced by the optimized position and setting of the PRVs.
Assessing Aspects of Cadmium Supply, Recycling and Environmental Pollution with Respect to Future Photovoltaic Technology Demands and Envionmental Policy Goals
Authors: Sverdrup Harald Ulrik , van Allen Ole , Haraldsson Hördur Valdimar 
Published: April 2024

AbstractCadmium has appeared as an important element for certain types of solar cells and rechargeable batteries. It is possible that there will be a large increase in demand for technical cadmium in the future. This is in conflict with environmental policies for phasing out cadmium from any technical use worldwide because of its great toxicity to humans. Cadmium toxicity is on par with that of mercury, and data suggests that cadmium exposure has no safe lower limit. There is no shortage of cadmium to extract, and no shortage from lack of cadmium available in the future zinc flow is to be expected. There is a global treaty to ban it from all use. The Integrated Assessment Model WORLD7 was used to assess different aspects of the supply of cadmium to society. It would be possible to produce at least 250,000 tons/year; in reality, the 2023 production is about 24,000 tons/year. The price is about 3500–4500 $/ton and is volatile. Because there is a United Nations agreed global policy to phase out cadmium from all use, demand for cadmium will soon not be met, and there will be an actual shortage of cadmium for any use, including photovoltaic technologies and semiconductors. This is good news for nature, but bad news for the CdTe and CIGS types of photovoltaic panels. It is estimated that only 25% of the planned future capacity may not be available unless good substitutes for cadmium can be found.
Unpacking the Myth in the Associations Between Self-control and Gaming Disorder in Children and Adolescents: A Comparison Between Traditional and Random Intercept Cross-lagged Panel Model Analyses
Authors: QI Di , LI Xiaomin , ZHU Shimin 
Published: April 2024

AbstractInternet gaming is becoming increasingly popular; however, children and adolescents are highly vulnerable to gaming disorder due to the underdevelopment of cognitive control. Longitudinal research providing empirical evidence confirming the stability and direction of the association between self-control and gaming disorder is scarce. This study is aimed at clarifying whether prospective relationships exist between self-control and gaming disorder in children and adolescents or whether they are associated due to common causes. We examined the temporal dynamics of the associations between self-control and gaming disorder symptom severity using a traditional cross-lagged panel model (CLPM; aggregating between- and within-person variance) and a random intercept CLPM (RI-CLPM; disaggregating between- and within-person effects) with three-annual wave data from a large cohort of primary and secondary students (N = 1359, mean age 12.67 (SD 1.40), 834 (61.7%) girls). The traditional CLPM indicated a unidirectional negative relationship from self-control to later gaming disorder (B with 95% confidence interval [CI] =  − 0.12 [− 0.19, − 0.040]), whereas the RI-CLPM analysis revealed no prospective relationship between self-control and gaming disorder (95% CIs of Bs all contain 0), indicating that self-control was neither the cause nor the result of gaming disorder in children and adolescents. Our study revealed that the nature of the relationship between self-control and gaming disorder tends to be correlational but not causal. The potential common underlying factors for future research are discussed.
Selection and characterization of lead-tolerant sweetpotato cultivars for phytoremediation
Authors: Daurov Dias , Lim Ye-Hoon , Park Sul-U , Kim Yun-Hee , Daurova Ainash , Sapakhova Zagipa , Zhapar Kuanysh , Abilda Zhanar , Toishimanov Maxat , Shamekova Malika , Zhambakin Kabyl , Kim Ho Soo , Kwak Sang-Soo 
Published: April 2024

AbstractLead (Pb) is one of the most toxic heavy metals (HMs) for plants and the environment. Sweetpotato [Ipomoea batatas (L.) Lam], the sixth most important food crop in the world, is tolerant to various environmental stresses, owing to its high antioxidant capacity. In this study, we selected sweetpotato cultivars showing high tolerance to lead (Pb) for phytoremediation-related applications. Young seedlings of 20 sweetpotato cultivars were treated with 30 mM Pb. Daeyumi (KO-12) and Dahomi (KO-5) were selected as Pb-tolerant and -sensitive cultivars, respectively, based on their photosynthetic activity and growth inhibition index (I50). In the Pb treatment, hydrogen peroxide and malondialdehyde contents of KO-12 were 1.5-fold less than those of KO-5. In addition, KO-12 showed a higher ability to accumulate Pb in roots and leaves than KO-5. Expression levels of four Pb-responsive genes, including the metallothionein gene IbMT1, were higher in the roots and leaves of KO-12 than in those of KO-5. Interestingly, KO-12 showed greater tolerance to high Pb concentrations than sunflower and rapeseed, which have been well-studied for phytoremediation. Our results suggest that sweetpotato is a suitable biomaterial for the phytoremediation of soils contaminated with HMs, including lead, for sustainable agriculture.
Workaholism and workplace incivility: a moderated dual-process model
Authors: Liu Xiayi , He Peixu , Jiang Shaojing 
Published: April 2024

AbstractDespite the ubiquity of workaholism and workplace incivility, extant research lacks sufficient empirical support on the underlying mechanisms between them, which hinders curtailing the uncivil behavior of workaholics. To systematically investigate the underlying mechanisms, we proposed two mediators: emotional exhaustion and psychological entitlement. The former illustrates why workaholics engage in uncivil behaviors uncontrollably from the existing conservation of resources perspective, which captures the behavioral dimension of workaholism. The latter explains why workaholics engage in workplace incivility voluntarily from a novel moral licensing perspective, which captures the overlooked cognitive dimension of workaholism. Further, we incorporate supervisor-subordinate guanxi as a critical moderator that helps differentiate the above two mediators. Results across two studies suggested that supervisor-subordinate guanxi alleviates the indirect effects of workaholism on workplace incivility via emotional exhaustion, while magnifying the indirect effects via psychological entitlement. Overall, these findings provide evidence that workaholism can also psychologically free employees to engage in subsequent uncivil behaviors.
Efficient single-pixel imaging based on a compact fiber laser array and untrained neural network
Authors: Lai Wenchang , Lei Guozhong , Meng Qi , Wang Yan , Ma Yanxing , Liu Hao , Cui Wenda , Han Kai 
Published: April 2024

AbstractThis paper presents an efficient scheme for single-pixel imaging (SPI) utilizing a phase-controlled fiber laser array and an untrained deep neural network. The fiber lasers are arranged in a compact hexagonal structure and coherently combined to generate illuminating light fields. Through the utilization of high-speed electro-optic modulators in each individual fiber laser module, the randomly modulated fiber laser array enables rapid speckle projection onto the object of interest. Furthermore, the untrained deep neural network is incorporated into the image reconstructing process to enhance the quality of the reconstructed images. Through simulations and experiments, we validate the feasibility of the proposed method and successfully achieve high-quality SPI utilizing the coherent fiber laser array at a sampling ratio of 1.6%. Given its potential for high emitting power and rapid modulation, the SPI scheme based on the fiber laser array holds promise for broad applications in remote sensing and other applicable fields.
Graphical Abstract
L-cysteine contributes to destructive activities of odontogenic cysts/tumor
Authors: Li Ji , Feng Chunyu , Pang Xiaochan , Li Xiang , Dou Xinyu , Jiang Erhui , Shang Zhengjun 
Published: April 2024

Abstract
Background
Odontogenic cysts/tumor can cause severe bone destruction, which affects maxillofacial function and aesthetics. Meanwhile, metabolic reprogramming is an important hallmark of diseases. Changes in metabolic flow affect all aspects of disease, especially bone-related diseases. At present, the researches on pathogenesis of odontogenic cysts/tumor are mainly focused on the level of gene regulation, but the effects of metabolic alterations on odontogenic cysts/tumor have still underexplored.

Materials and methods
Imaging analysis was used to evaluate the lesion size of different odontogenic lesions. Tartrate resistant acid phosphatase (TRAP) and immunohistochemistry (IHC) assays were utilized to detect the differences in bone destruction activity in odontogenic cysts and tumors. Furthermore, metabolomics and weighted gene co-expression network analysis (WGCNA) were conducted for the metabolomic features and key metabolite screening, respectively. The effect of ferroptosis inhibition on bone destruction was confirmed by IHC, immunofluorescence, and malondialdehyde colorimetric assay.

Results
The bone destruction activity of ameloblastoma (AM) was the strongest and the weakest in odontogenic cysts (OC). High-throughput targeted metabolomics was used to map the metabolomic profiles of OC, odontogenic keratocyst (OKC) and AM. WGCNA and differential analysis identified L-cysteine in OKC and AM. Cystathionine γ-lyase (CTH) was further screened by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The functions of L-cysteine were further validated. Finally, we confirmed that CTH affected destructive activities by regulating the sensitivity of epithelial cells to ferroptosis.

Conclusion
High-throughput targeted metabolomics performed on diseased tissue confirmed the unique alteration of metabolic profiles in OKC and AM. CTH and its metabolite L-cysteine are the key factors regulating destructive activities.