bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma
Authors: Valero-Rivera Karla Paulina , Magaña-Aquino Martin , del Carmen Romero-Méndez María , Morales-Barragán Miriam Nayeli , Sagahón-Azúa Julia , Medellín-Garibay Susanna Edith , del Carmen Milán-Segovia Rosa , Martínez-Gutiérrez Fidel , Romano-Moreno Silvia 
Published: March 2024

Abstract
A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50–25 μg/mL and with a correlation coefficient (r2) &gt;0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation &lt;15%. Excellent recovery in the range of 90–104% was achieved for CTB and SUL was 86–110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies.
Morphological and dynamic analysis of the normal aortic valve with 4D computed tomography
Authors: Fikani Amine , Craiem Damian , Mousseaux Elie , Soulat Gilles , Rouchaud Aymeric , Boulogne Cyrille , Martins Elie , Jouan Jerome 
Published: March 2024

Abstract

OBJECTIVES
To evaluate the precise dimensions of the normal aortic root, especially the true aortic annulus, during the cardiac cycle using an innovative reconstruction method based on multiphase cardiac computed tomography and to assess the feasibility and the reproducibility of this method for aortic root analysis.

METHODS
Between January 2019 and June 2021, 30 optimal consecutive ECG-gated multiphase cardiac computed tomography of patients with normal tricuspid aortic valve were analysed using an in-house software. Aortic annulus border was pinpointed on 9 reconstructed planes and the 3D coordinates of the 18 consecutive points were interpolated into a 3D curve using a cubic spline. Three additional planes were generated at the level of the left ventricular outflow tract, the level of the Valsalva sinus and the level of the sinotubular junction. This procedure was repeated for all the 10 temporal phases of the RR interval.

RESULTS
The aortic annulus mean 3D and 2D areas were 7.67 ± 1.51 and 5.16 ± 1.40 cm2, respectively. The mean 2D diameter was 2.51 ± 0.23 cm. The mean global area expansion was 11.8 ± 3.5% and the mean perimeter expansion of 7.1 ± 2.6%. During the cardiac cycle, the left ventricle outflow tract expands, reaching its maximum surface at the end of diastole, followed by the aortic annulus, the Valsalva sinuses and the sinotubular junction. The aorta changes from a clover-shaped cone during diastole to more cylindrical shape during systole. Compared to the 3D measurements, the analysis of the virtual basal ring significantly underestimates the annulus area, perimeter, and mean diameter.

CONCLUSIONS
4D morphometric analysis enables to have a precise and reproducible evaluation of the aortic annulus. The aortic annulus and root are deformable structures that undergo a unique expansion sequence during the cardiac cycle which should be considered for procedural planning.
Dealing with the doctor shortage: a qualitative study exploring French general practitioners’ lived experiences, difficulties, and adaptive behaviours
Authors: Dumesnil Hélène , Lutaud Romain , Bellon-Curutchet Julien , Deffontaines Aliénor , Verger Pierre 
Published: March 2024

Abstract

Context
The shortage of general practitioners (GPs) is a growing concern in Europe, especially in France. This problem is likely to continue until the end of the 2020s.

Objectives
To study the GPs’ perceptions of access to care in medically underserved areas (i.e. with low physician density), its consequences on their working conditions, and how they cope with the resulting difficulties.

Methods
Semi-structured individual interviews were conducted between May and August 2021 of 29 GPs practising in areas of southeastern France with a low physician density or at risk of a doctor shortage. Purposive sampling was used to include profiles of diverse physicians and diverse rural and urban areas. The interviews, conducted with an interview guide, were transcribed and analysed thematically.

Results
The participants described a serious degradation of access to care in their areas. These issues also concerned urban areas, where they were, according to the participants, underrecognized. The participants’ workloads were rising, at a rate often perceived as unsustainable: many participants, including the youngest group, reported they were exhausted. Their principal source of dissatisfaction was their impression that they could not do their work correctly. Participants reported that these difficulties required them to improvise and adapt without any official or formal method to keep their practice manageable.

Conclusion
These GPs were worried about the future of their profession and their patients. They expected strong measures by public policymakers and officials, but paradoxically seemed to have little interest in the solutions these officials are promoting.
Responses of attached bacterial communities to blooms of the swimming shelled pteropod Creseis acicula in Daya Bay, southern China
Authors: Shi Rongjun , Han Tingting , Qi Zhanhui , Huang Honghui 
Published: March 2024

Abstract
The shelled pteropod Creseis acicula is a marine pelagic shellfish widely distributed from temperate to tropical seas around the world. From June to July 2020, a C. acicula bloom first happened in the Daya Bay, southern China, and its density reached the highest value (5600 ind. m−3) ever recorded around the world. However, few studies have investigated the responses of bacterial communities to the C. acicula bloom. In the present study, we examined the community profiles of three communities of bacteria including the free-living and particle-attached bacteria in the blooming and reference waters, and bacteria attached to the whole body and shell of C. acicula using a high-throughput sequencing method. The results indicated that the C. acicula bloom had a greater impact on particle-attached bacteria than free-living bacteria. Among the bloom-sensitive particle-attached bacteria, the predominant bacterial phyla were Pseudomonadota, Bacteroidota and Verrucomicrobiota in the blooming areas, whereas they were Actinomycetota and Planctomycetota in the reference areas. Specifically, fecal bacteria Haloferula and Halioglobus spp. were significantly enriched in the blooming waters and accumulated on C. acicula shells. Conversely, the significantly lower relative abundance of Nocardioides sp. in the blooming area and accumulated on the whole body of C. acicula indicated their attachment to particles consumed by C. acicula. Overall, our results suggested that the C. acicula bloom influenced marine bacteria, particularly particle-attached bacteria, by increasing (e.g. providing shells and feces) or decreasing (e.g. filter-feeding the suspended particles) the abundance of available substances.
A new H∞ observer for continuous LPV systems with unknown inputs using an HOSM differentiator
Authors: Meyer Luc 
Published: March 2024

Abstract
The main contribution of this paper is the development of $H_{\infty }$ state and unknown input (UI) observers for noisy linear parameter-varying (LPV) systems. The observers are constructed in order to be unbiased (in particular the state estimation error is decoupled from the UI) and with a minimum $L_{2}$ transfer between the perturbations (that are assumed to be with finite energy) and the estimation errors. Contrary to equivalent observers developed in the literature, the present one relaxes a widely used rank condition on the system matrices for decoupling the UI. In order to do so, high-order derivation is needed, which is done using a high-order sliding modes differentiator. A method is given to design observer gains for LPV systems under polytopic form. Finally, three examples illustrate some aspects of the theoretical contributions, and compare this work to the existing ones.
Hierarchical control problem for the heat equation with dynamic boundary conditions
Authors: Oukdach Omar , Boutaayamou Idriss , Maniar Lahcen 
Published: March 2024

Abstract
This paper deals with a hierarchical control problem for the heat equation with dynamic boundary conditions. The main goal consists of letting the state near from a prescribed target in a fixed observation region. The secondary objective is the null controllability. In other words, we reverse the roles of the leader and the follower addressed in the recent article. For this purpose, we combine some appropriate Carleman estimates and the Stackelberg strategy. We also extend our study for hierarchical-biobjective problems by applying the Stackelberg–Pareto strategy.
Endosymbiont Tremblaya phenacola influences the reproduction of cotton mealybugs by regulating the mechanistic target of rapamycin pathway
Authors: Bai Jianyang , Zuo Zhangqi , DuanMu Haonan , Li Meizhen , Tong Haojie , Mei Yang , Xiao Yiqi , He Kang , Jiang Mingxing , Wang Shuping , Li Fei 
Published: March 2024

Abstract
The intricate evolutionary dynamics of endosymbiotic relationships result in unique characteristics among the genomes of symbionts, which profoundly influence host insect phenotypes. Here, we investigated an endosymbiotic system in Phenacoccus solenopsis, a notorious pest of the subfamily Phenacoccinae. The endosymbiont, “Candidatus Tremblaya phenacola” (T. phenacola PSOL), persisted throughout the complete life cycle of female hosts and was more active during oviposition, whereas there was a significant decline in abundance after pupation in males. Genome sequencing yielded an endosymbiont genome of 221.1 kb in size, comprising seven contigs and originating from a chimeric arrangement between betaproteobacteria and gammaproteobacteria. A comprehensive analysis of amino acid metabolic pathways demonstrated complementarity between the host and endosymbiont metabolism. Elimination of T. phenacola PSOL through antibiotic treatment significantly decreased P. solenopsis fecundity. Weighted gene coexpression network analysis demonstrated a correlation between genes associated with essential amino acid synthesis and those associated with host meiosis and oocyte maturation. Moreover, altering endosymbiont abundance activated the host mechanistic target of rapamycin pathway, suggesting that changes in the amino acid abundance affected the host reproductive capabilities via this signal pathway. Taken together, these findings demonstrate a mechanism by which the endosymbiont T. phenacola PSOL contributed to high fecundity in P. solenopsis and provide new insights into nutritional compensation and coevolution of the endosymbiotic system.
Public Opinion, Rivalry, and the Democratic Peace: Experimental Evidence from South Korea
Authors: Kim Gidong , Kim Yu Bin , Kwak Dongjin 
Published: March 2024

Abstract
Recent studies on the public opinion mechanism of the democratic peace have demonstrated experimentally that democratic citizens are averse to attacking other democracies. The presence of rivalry, however, has long been recognized as one of the important factors contributing to either initiation or recurrence of international conflict. Despite such importance, our understanding remains limited as to how rivalry affects public opinion, particularly in the context of the democratic peace. In this article, we argue that democratic publics’ perception of rivalry weakens the effect of regime type. We expect democratic publics to be less reluctant in terms of fighting other rival democracies. Using an original survey experiment in South Korea, we demonstrate that the South Korean public, similar to those of western democracies, is reluctant to use force against nonrival democracies, but less so against rival democracies. Our findings suggest that the scope of the democratic peace should be qualified.
5-hydroxytryphophan mitigates ergot alkaloid-induced suppression of serotonin and feed intake in cattle
Authors: Valente Eriton E L , Klotz James L , Markmann Ryana C , Edwards J Lannett , Harmon David L 
Published: March 2024

AbstractThe impact of ergot toxicosis on livestock industries is detrimental and treatments are needed in many countries. The objective of this study was to evaluate the effects of acute exposure to ergot alkaloids and 5-hydroxytryptophan (5-HTP) supplementation on feed intake, serotonin metabolism, and blood metabolites in cattle. Eight Holstein steers (538 ± 18 kg) fitted with ruminal cannulas were used in a replicated 4 × 4 Latin Square design experiment with a 2 × 2 factorial treatment structure. The treatments were the combination of 0 (E−) or 15 µg ergovaline/kg BW (E+) and 0 (5HTP−) or 0.5 mg of 5-hydroxy-l-tryptophan/kg BW (5HTP+) administered daily for 6 d. Toxic endophyte-infected tall fescue seed was used to supply the daily dose of ergovaline. Endophyte-free seed was used to equalize seed intake between treatments. Ground seed was placed into the rumen immediately before feeding. The 5-HTP was dissolved in water and infused into the abomasum via the reticulo-omasal orifice. Blood was collected from a jugular vein catheter at 0, 1, 2, 4, 8, and 24 h after treatment administration. Ergovaline without 5-HTP (E+/5HTP−) decreased dry matter intake (DMI) in comparison to steers without ergovaline and 5-HTP (E-/5HTP−). However, 5-HTP infusion in association with ergovaline (E+/5HTP+) normalized the DMI. Although E + did not affect (P &gt; 0.05) the area under the curve (AUC) of serum 5-HTP, 5-hydroxyindoleacetic acid, tryptophan, and kynurenine, serum and plasma serotonin concentrations were decreased (P &lt; 0.05). The infusion of 5-HTP increased (P &lt; 0.05) the AUC of serum 5-HTP, serum and plasma serotonin, and serum 5-hydroxyindoleacetic acid. In conclusion, acute exposure to ergot alkaloids reduced DMI and circulating serotonin in cattle but 5-HTP administration showed potential to normalize both circulating serotonin and feed intake.
From Admission to Discharge—A Total Friction Burn Review from a Single Institution
Authors: Estrada Munoz Oscar I , Sabour Andrew F , Carroll Joseph T , Flores Carmen E , Fraser Douglas R , Chestovich Paul , Saquib Syed F 
Published: March 2024

Abstract
While most friction burns are adequately managed in an outpatient setting, many may require hospital admission, operative excision, and extended care. To this day, there is a wide variance in friction burn management. Our goal is to review the etiology, management, and outcomes of such burns warranting hospitalization. We conducted a retrospective review of all friction burns admitted to a single, American Burn Association-verified burn center from January 1, 2016 to December 31, 2020. A total of 28 (34%) patients required surgery for their friction burns and 15 (18%) ultimately required a split-thickness skin graft. The mean number of operations was 2.4 (95% CI 1.6-3.1). Overall, the operative group was younger (29.9 vs 38.3 years, P = .026), more likely to have a concomitant traumatic brain injury (25% vs 7%, P = .027), and had a longer hospital length of stay (17.5 vs 3.9 days, P &lt; .001). Both groups had a similar overall TBSA (8.5% vs 10.0%, P = .35), but the operative group had a larger surface area comprised of third-degree burns (3.05% vs 0.2%, P &lt; .001). Overall, friction burns resulting in hospital admission are associated with high-energy traumatic mechanisms and concomitant injuries. Patients who need operative intervention for their burns typically require multiple procedures often culminating in a split-thickness skin graft. While non-operative management of friction burns with topical agents has been found to be successful, patients with higher injury severity scores should be monitored very closely as they may require surgical excision.
Improving full-waveform inversion based on sparse regularization for geophysical data
Authors: Li Jiahang , Mikada Hitoshi , Takekawa Junichi 
Published: March 2024

Abstract
Full-waveform inversion (FWI) is an advanced geophysical inversion technique. FWI provides images of subsurface structures with higher resolution in fields such as oil exploration and geology. The conventional algorithm minimizes the misfit error by calculating the least squares of the wavefield solutions between observed data and simulated data, followed by gradient direction and model update increment. Since the gradient is calculated by forward and backward wavefields, the high-accuracy model update relies on accurate forward and backward wavefield modelling. However, the quality of wavefield solutions obtained in practical situations could be poor and does not meet the requirements of high-resolution FWI. Specifically, the low-frequency wavefield is easily affected by noise and downsampling, which influences data quality, whereas the high-frequency wavefield is susceptible to spatial aliasing effects that produce imaging artefacts. Therefore, we propose using an algorithm called sparse relaxation regularized regression to optimize the wavefield solution in frequency-domain FWI, which is the forward and backward wavefield obtained from the Helmholtz equation, thus improving FWI's accuracy. The sparse relaxation regularized regression algorithm combines sparsity and regularization, allowing the broadband FWI to reduce the effects of noise and outliers, which can provide data supplementation in the low-frequency band and anti-aliasing in the high-frequency band. Our numerical examples demonstrate the wavefield optimization effect of the sparse relaxation regularized regression-based algorithm in various cases. The improved algorithm's accuracy and stability are verified compared to the Tikhonov regularization algorithm.
AGV fuzzy control optimized by genetic algorithms
Authors: Sierra-Garcia J Enrique , Santos Matilde 
Published: March 2024

Abstract
Automated Guided Vehicles (AGV) are an essential element of transport in industry 4.0. Although they may seem simple systems in terms of their kinematics, their dynamics is very complex, and it requires robust and efficient controllers for their routes in the workspaces. In this paper, we present the design and implementation of an intelligent controller of a hybrid AGV based on fuzzy logic. In addition, genetic algorithms have been used to optimize the speed control strategy, aiming at improving efficiency and saving energy. The control architecture includes a fuzzy controller for trajectory tracking that has been enhanced with genetic algorithms. The cost function first maximizes the time in the circuit and then minimizes the guiding error. It has been validated on the mathematical model of a commercial hybrid AGV that merges tricycle and differential robot components. This model not only considers the kinematics and dynamics equations of the vehicle but also the impact of friction. The performance of the intelligent control strategy is compared with an optimized PID controller. Four paths were simulated to test the approach validity.
Emerging measurements for tumor-infiltrating lymphocytes in breast cancer
Authors: Wu Rongrong , Horimoto Yoshiya , Oshi Masanori , Benesch Matthew G K , Khoury Thaer , Takabe Kazuaki , Ishikawa Takashi 
Published: March 2024

Abstract
Tumor-infiltrating lymphocytes are a general term for lymphocytes or immune cells infiltrating the tumor microenvironment. Numerous studies have demonstrated tumor-infiltrating lymphocytes to be robust prognostic and predictive biomarkers in breast cancer. Recently, immune checkpoint inhibitors, which directly target tumor-infiltrating lymphocytes, have become part of standard of care treatment for triple-negative breast cancer. Surprisingly, tumor-infiltrating lymphocytes quantified by conventional methods do not predict response to immune checkpoint inhibitors, which highlights the heterogeneity of tumor-infiltrating lymphocytes and the complexity of the immune network in the tumor microenvironment. Tumor-infiltrating lymphocytes are composed of diverse immune cell populations, including cytotoxic CD8-positive T lymphocytes, B cells and myeloid cells. Traditionally, tumor-infiltrating lymphocytes in tumor stroma have been evaluated by histology. However, the standardization of this approach is limited, necessitating the use of various novel technologies to elucidate the heterogeneity in the tumor microenvironment. This review outlines the evaluation methods for tumor-infiltrating lymphocytes from conventional pathological approaches that evaluate intratumoral and stromal tumor-infiltrating lymphocytes such as immunohistochemistry, to the more recent advancements in computer tissue imaging using artificial intelligence, flow cytometry sorting and multi-omics analyses using high-throughput assays to estimate tumor-infiltrating lymphocytes from bulk tumor using immune signatures or deconvolution tools. We also discuss higher resolution technologies that enable the analysis of tumor-infiltrating lymphocytes heterogeneity such as single-cell analysis and spatial transcriptomics. As we approach the era of personalized medicine, it is important for clinicians to understand these technologies.
Evaluating the carnivorous efficacy ofUtricularia aurea(Lamiales: Lentibulariaceae) on the larval stages ofAnopheles stephensi,Culex quinquefasciatus, andAedes aegypti(Diptera: Culicidae)
Authors: Mohanty Ajeet Kumar , Govekar Abhishek , de Souza Charles , Mohapatra Abhipsa , Janarthanam Malapati Kuppuswamy , Vukanti Raja , Montemarano Justin J , Balabaskaran Nina Praveen 
Published: March 2024

AbstractThe emergence of insecticide resistance in mosquitoes necessitates the exploration and validation of sustainable biological strategies for controlling mosquitoes in their natural habitats. We assessed the predatory effect of Utricularia aurea Lour (Lamiales: Lentibulariaceae), an aquatic carnivorous plant found in the Indian subcontinent, Japan, and Australia, on 4 instars of Anopheles stephensi Liston, Culex quinquefasciatus Say, and Aedes aegypti Linn (Diptera: Culicidae), in the laboratory and field settings. In the laboratory setting, predation of larvae by U. aurea was highest during the first hour when it predated 45%, 61%, and 58% of first instars of An. stephensi, Cx. quinquefasciatus, and, Ae. aegypti, respectively, and, within 12 h, U. aurea preyed upon ~95% of the first, second, and third instars of the 3 mosquito species, ~80% of the fourth instars of An. stephensi and Ae. aegypti, and ~60% of fourth instars of Cx. quinquefasciatus. The predatory effect of U. aurea varied with mosquito species and instar. Broadly, predation risk declined with the increase of the instar size. In the field setting, at the end of 16 days, U. aurea predated 76% and 71% of the immature An. stephensi and Ae. aegypti, respectively. Our findings suggest U. aurea can be utilized as a potential biocontrol agent for controlling mosquito larvae in natural habitats; however, the current claim warrants additional investigations in a variety of natural habitats.