bluuebunny/crossref_metadata_2025_split huggingface dataset:
An N‐terminal diacidic motif is required for the trafficking of maize aquaporins ZmPIP2;4 and ZmPIP2;5 to the plasma membrane
Authors: Zelazny Enric , Miecielica Urszula , Borst Jan Willem , Hemminga Marcus A. , Chaumont François 
Published: September 2008

SummaryMaize plasma membrane aquaporins (ZmPIPs, where PIP is the plasma membrane intrinsic protein) fall into two groups, ZmPIP1s and ZmPIP2s, which, when expressed alone in mesophyll protoplasts, are found in different subcellular locations. Whereas ZmPIP1s are retained in the endoplasmic reticulum (ER), ZmPIP2s are found in the plasma membrane (PM). We previously showed that, when co‐expressed with ZmPIP2s, ZmPIP1s are relocalized to the PM, and that this relocalization results from the formation of hetero‐oligomers between ZmPIP1s and ZmPIP2s. To determine the domains responsible for the ER retention and PM localization, respectively, of ZmPIP1s and ZmPIP2s, truncated and mutated ZmPIPs were generated, together with chimeric proteins created by swapping the N‐ or C‐terminal regions of ZmPIP2s and ZmPIP1s. These mutated proteins were fused to the mYFP and/or mCFP, and the fusion proteins were expressed in maize mesophyll protoplasts, and were then localized by microscopy. This allowed us to identify a diacidic motif, DIE (Asp‐Ile‐Glu), at position 4–6 of the N‐terminus of ZmPIP2;5, that is essential for ER export. This motif was conserved and functional in ZmPIP2;4, but was absent in ZmPIP2;1. In addition, we showed that the N‐terminus of ZmPIP2;5 was not sufficient to cause the export of ZmPIP1;2 from the ER. A study of ZmPIP1;2 mutants suggested that the N‐ and C‐termini of this protein are probably not involved in ER retention. Together, these results show that the trafficking of maize PM aquaporins is differentially regulated depending on the isoform, and involves a specific signal and mechanism.
The BTB ubiquitin ligases ETO1, EOL1 and EOL2 act collectively to regulate ethylene biosynthesis in Arabidopsis by controlling type‐2 ACC synthase levels
Authors: Christians Matthew J. , Gingerich Derek J. , Hansen Maureen , Binder Brad M. , Kieber Joseph J. , Vierstra Richard D. 
Published: September 2008

SummaryEthylene biosynthesis is directed by a family of 1‐aminocyclopropane‐1‐carboxylic acid (ACC) synthases (ACS) that convert S‐adenosyl‐l‐methionine to the immediate precursor ACC. Members of the type‐2 ACS subfamily are strongly regulated by proteolysis with various signals stabilizing the proteins to increase ethylene production. In Arabidopsis, this turnover is mediated by the ubiquitin/26 S proteasome system, using a broad complex/tramtrack/bric‐a‐brac (BTB) E3 assembled with the ETHYLENE OVERPRODUCER 1 (ETO1) BTB protein for target recognition. Here, we show that two Arabidopsis BTB proteins closely related to ETO1, designated ETO1‐like (EOL1) and EOL2, also negatively regulate ethylene synthesis via their ability to target ACSs for breakdown. Like ETO1, EOL1 interacts with type‐2 ACSs (ACS4, ACS5 and ACS9), but not with type‐1 or type‐3 ACSs, or with type‐2 ACS mutants that stabilize the corresponding proteins in planta. Whereas single and double mutants affecting EOL1 and EOL2 do not show an ethylene‐related phenotype, they exaggerate the effects caused by inactivation of ETO1, and further increase ethylene production and the accumulation of ACS5 in eto1 plants. The triple eto1 eol1 eol2 mutant phenotype can be effectively rescued by the ACS inhibitor aminoethoxyvinylglycine, and by silver, which antagonizes ethylene perception. Together with hypocotyl growth assays showing that the sensitivity and response kinetics to ethylene are normal, it appears that ethylene synthesis, but not signaling, is compromised in the triple mutant. Collectively, the data indicate that the Arabidopsis BTB E3s assembled with ETO1, EOL1 and EOL2 work together to negatively regulate ethylene synthesis by directing the degradation of type‐2 ACS proteins.
Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter
Authors: Hayashi Keiko , Yoshida Hitoshi 
Published: September 2008

SummaryThe plant genome contains a large number of disease resistance (R) genes that have evolved through diverse mechanisms. Here, we report that a long terminal repeat (LTR) retrotransposon contributed to the evolution of the rice blast resistance gene Pit. Pit confers race‐specific resistance against the fungal pathogen Magnaporthe grisea, and is a member of the nucleotide‐binding site leucine‐rich repeat (NBS‐LRR) family of R genes. Compared with the non‐functional allele PitNpb, the functional allele PitK59 contains four amino acid substitutions, and has the LTR retrotransposon Renovator inserted upstream. Pathogenesis assays using chimeric constructs carrying the various regions of PitK59 and PitNpb suggest that amino acid substitutions might have a potential effect in Pit resistance; more importantly, the upregulated promoter activity conferred by the Renovator sequence is essential for Pit function. Our data suggest that transposon‐mediated transcriptional activation may play an important role in the refunctionalization of additional ‘sleeping’R genes in the plant genome.
CLINICAL STUDY: The P300 event‐related potential and its possible role as an endophenotype for studying substance use disorders: a review
Authors: Singh Shubh M. , Basu Debasish 
Published: September 2008

ABSTRACTThe concept of endophenotypes has gained popularity in recent years. This is because of the potential that endophenotypes provide of measuring objective trait markers that are simpler to access and assess than complex behavioral disease phenotypes themselves. The simplicity, ease of measurement and the putative links to the etiology of the disease in the study of an endophenotype has the potential promise of unraveling the genetic basis of the disease in question. Of the various proposed endophenotypes, the P300 component of the event‐related potential has been used in studies on alcoholism, schizophrenia and externalizing disorders. The current state of knowledge regarding the concept of endophenotypes, P300 and the validity of P300 as an endophenotype with special reference to substance use disorders is discussed in this review. The implications of the above are discussed.
Pseudomonas aeruginosa infection after living‐donor liver transplantation in adults
Authors: Hashimoto M. , Sugawara Y. , Tamura S. , Kaneko J. , Matsui Y. , Kokudo N. , Makuuchi M. 
Published: September 2008

Objectives. Pseudomonas aeruginosa infection is a major cause of bacterial infection after deceased‐donor liver transplantation. The incidence and risk factors of P. aeruginosa infection after living‐donor liver transplantation (LDLT), however, are not known.Methods. We retrospectively reviewed the data from 170 adult patients who underwent LDLT at the University of Tokyo Hospital. The microbiologic and medical records of the patients from admission to 3 months after LDLT were reviewed. Uni‐ and multivariate analyses were performed to identify the independent risk factors for postoperative P. aeruginosa infection.Result. Preoperative P. aeruginosa carriage was identified in 15 (9%) patients. Only 2 of the 15 patients later presented with postoperative P. aeruginosa infection. Postoperative P. aeruginosa infection occurred in 27 (16%) of 170 patients by median postoperative day 38. Among those 27 patients, surgical site infections were recorded in 8 (30%) and intra‐abdominal infections in 14 (52%). In 5 of the 27 (19%) patients, P. aeruginosa isolates were multiple antimicrobial resistant. Postoperative bile leakage independently predicted postoperative P. aeruginosa infection.Conclusion. P. aeruginosa infections were frequently detected after LDLT, including those by multiple antimicrobial‐resistant isolates. Postoperative bile leakage predisposed patients to P. aeruginosa infection. Surveillance culture should be checked periodically after LDLT to ensure that appropriate antimicrobials can be administered for postoperative infection.
Extraovarian pelvic yolk sac tumor: Case report and review of published work
Authors: Pasternack Tanya , Shaco‐Levy Ruthy , Wiznitzer Arnon , Piura Benjamin 
Published: September 2008

AbstractExtraovarian pelvic yolk sac tumors are rare, with only nine cases documented previously in the published work. We report a case of extraovarian pelvic yolk sac tumor occurring synchronously with breast carcinoma. The patient underwent resection of the pelvic tumor and hepatic implant, omentectomy and breast lumpectomy with sentinel axillary lymph node biopsy. The uterus and bilateral adnexa were preserved. Postoperative adjuvant therapy for the yolk sac tumor included three cycles of the bleomycin, etoposide and cisplatin (BEP) regimen. This was followed by adjuvant chemotherapy and radiotherapy for the breast carcinoma. It is concluded that in women with extraovarian pelvic yolk sac tumor who wish to preserve childbearing capacity, fertility‐saving surgery followed by fertility‐preserving cisplatin‐based chemotherapy is adequate and appropriate treatment.
Recurrent Kikuchi–Fujimoto disease during pregnancy: Report of case evolving into systemic lupus erythematosus and review of published work
Authors: Alijotas‐Reig Jaume , Casellas‐Caro Manel , Ferrer‐Oliveras Raquel , Cabero‐Roura Lluis , Vilardell‐Tarres Miquel 
Published: September 2008

AbstractKikuchi–Fujimoto disease (KFD) is a distinctive, benign type of necrotizing lymphadenitis. KFD is a rare entity, especially during pregnancy. Although first described in Japan, subsequently it has been reported in the West. We communicate herein a case of KFD in African women with bouts during pregnancy. Diagnosis was difficult because peripheral enlarged lymph nodes were absent and an extended differential diagnosis and adequate work‐up was necessary. The patient suffered a miscarriage coinciding with a KFD bout. Further pregnancies were uneventful. Follow up showed that KFD evolved into a systemic lupus erythematosus‐like syndrome.
Real‐time three‐dimensional color Doppler fetal echocardiographic features of congenital heart disease
Authors: Hata Toshiyuki , Dai Shu‐Yan , Inubashiri Eisuke , Kanenishi Kenji , Tanaka Hirokazu , Yanagihara Toshihiro , Nukazawa Takashi 
Published: September 2008

AbstractThe purpose of this report is to describe the real‐time three‐dimensional (3D) color Doppler fetal echocardiographic findings of congenital heart diseases. Two cases of fetal congenital heart disease were diagnosed antenatally by conventional two‐dimensional fetal echocardiography and real‐time 3D color Doppler fetal echocardiography. Conventional two‐dimensional (2D) fetal echocardiography showed a hypoplastic left heart in one case, and a double‐outlet right ventricle in the second case. Real‐time 3D fetal echocardiography with instantaneous volume‐rendered displays showed surgeon's eye views of intracardiac abnormal cardiac structures of the beating heart in both cases. Real‐time 3D color Doppler fetal echocardiography depicted a subtle tricuspid regurgitant jet in hypoplastic left heart syndrome, and great arteries, left from the right ventricle, in parallel in a double‐outlet right ventricle. Real‐time 3D color Doppler fetal echocardiography may assist in the evaluation of fetal cardiac anatomy and hemodynamics, and offer the potential advantages relative to conventional 2D fetal echocardiography and Doppler flow mapping.
Occipital scalp hemangioma: Prenatal sonographic and magnetic resonance images
Authors: Miyakoshi Kei , Tanaka Mamoru , Matsumoto Tadashi , Hattori Yoshihisa , Minegishi Kazuhiro , Ishimoto Hitoshi , Yoshimura Yasunori 
Published: September 2008

AbstractFetal scalp hemangioma in the occipital region is extremely rare and its accurate diagnosis is essential for perinatal management. We present a case of occipital scalp hemangioma diagnosed by prenatal sonography and magnetic resonance imaging (MRI). An echogenic mass measuring 29 mm × 23 mm × 30 mm was found in the occipital region by sonography at 20 weeks of gestation. Color‐flow Doppler sonography depicted vascularity only at the periphery of the mass. The MRI results indicated that the extracranial mass exhibited a heterogeneous appearance of predominantly hyperintensity on T1‐ and hypointensity on T2‐weighted images without evidence of fat signals, suggesting a soft tissue lesion containing blood products. Based on sonographic and MRI findings, the fetus was diagnosed to have occipital scalp hemangioma. Postmortem examination revealed cavernous hemangioma with hemorrhage. The MRI was a valuable adjunct to sonography for prenatal evaluation of an occipital lesion.
Prospective comparison of delivery outcomes of vaginal births after cesarean section versus laparoscopic myomectomy
Authors: Makino Shintaro , Tanaka Toshitaka , Itoh Shigeru , Kumakiri Jun , Takeuchi Hiroyuki , Takeda Satoru 
Published: September 2008

AbstractAim:  This study was designed to assess the delivery outcomes of vaginal birth after cesarean section in comparison with delivery after laparoscopic myomectomy.Methods:  The following data were collected: the proportion of patients who attempted vaginal birth, success rate, maternal age, previous number of vaginal deliveries, gestational weeks, birthweight, Apgar score, umbilical blood pH, and duration of labor in cases with a successful vaginal birth.Results:  The proportion of patients who attempted the vaginal birth differed significantly between the two groups (P < 0.0001). There were no significant differences in success rate, maternal age, number of previous vaginal deliveries, gestational weeks, birthweight, Apgar score or umbilical blood pH, although the mean duration of labor differed significantly (P = 0.006).Conclusions:  The present investigation confirmed that vaginal birth after laparoscopic myomectomy is as safe as vaginal birth after cesarean section.
Pregnancy complicated by Caroli's disease with polycystic kidney disease: A case report and following observations
Authors: Tsunoda Mika , Ohba Takashi , Uchino Kikuko , Katabuchi Hidetaka , Okamura Hitoshi , Kaneki Shizuo 
Published: September 2008

AbstractCaroli's disease and Caroli's syndrome are rare congenital disorders characterized by non‐obstructive cystic dilatation of the intrahepatic bile ducts. These disorders are often associated with autosomal recessive polycystic kidney disease. A young woman at 11 weeks of gestation was referred to our hospital for proper management of Caroli's disease during pregnancy. Magnetic resonance imaging and laboratory tests revealed Caroli's disease with chronic renal failure caused by polycystic kidney disease. She received diet control, erythropoietin and prophylactic oral antibiotics. Her pregnancy course was uneventful, and she gave birth at 37 weeks of gestation. Thereafter, her renal function gradually worsened. Hemodialysis was begun 5 years after parturition. Though the courses of pregnancies complicated by Caroli's disease or Caroli's syndrome are variable and can include life‐threatening conditions, uneventful outcomes can be expected if careful management prevents biliary and renal infection.
Two cases of infants who needed cardiopulmonary resuscitation during early skin‐to‐skin contact with mother
Authors: Nakamura Tomohiko , Sano Yoko 
Published: September 2008

AbstractEarly skin‐to‐skin contact (SSC) results in significantly better overall performance on all measures of breastfeeding status and duration. SSC is widely believed to be free of adverse effects. We recently encountered two cases having life‐threatening episodes during SSC. An experienced nurse should be placed in the delivery room for periodic observation and recording of vital signs of newborns who are in SSC (color, respirations, tone and heart rate) and to alert the physician to any cardiorespiratory changes. The point should also be emphasized to mothers that infants must be observed carefully and medical staff called immediately if the infant displays any vital changes during SSC.
Successful management by recombinant activated factor VII in a case of disseminated intravascular coagulopathy caused by obstetric hemorrhage
Authors: Nohira Tomoyoshi , Osakabe Yumi , Suda Shingo , Takahashi Chie , Tanaka Asashi , Ikeda Kazumi , Ikeda Toshiaki , Okabe Kazuhiro 
Published: September 2008

AbstractPostpartum hemorrhage (PPH) is a life‐threatening emergency in obstetrics. Although recombinant activated factor VII (rFVIIa) has become used for the treatment of some cases of massive hemorrhage, its applications in the field of obstetrics are still limited. We describe a case of successful treatment with rFVIIa for PPH due to placenta accreta. The patient was a 33‐year‐old woman with placental previa. Cesarean section (CS) was performed at gestational week 35. During CS, there was massive hemorrhage due to placenta accreta. After CS, disseminated intravascular coagulopathy and hypovolemic shock were diagnosed. The PPH was not controlled by transfusion therapy. On the fourth day after CS, rFVIIa (90 µg/kg × 2) was given because of the persistent PPH. Bleeding decreased and no further transfusion was required from 2 days after administration. rFVIIa was useful in the treatment of this case of obstetric hemorrhage.
Prenatal diagnosis of congenital cystic adenomatoid malformation using three‐dimensional inversion rendering: A case report
Authors: Sanz‐Cortés Magdalena , Raga Francisco , Bonilla‐Musoles Fernando 
Published: September 2008

AbstractWe report a case of a congenital cystic adenomatoid malformation of the lung (CCAM). At 12 weeks of gestation, an echogenic lung associated to a hydrothorax was detected. Despite the results of the combined test that informed of a high risk of chromosomopathy, normal karyotype was confirmed after an amniocentesis. The three‐dimensional ultrasound inversion mode depicted all the cysts within the fetal lungs as opaque areas that were seen concurrently together, which was compatible with CCAM. After parental counseling, the patient opted to terminate the pregnancy at 18 weeks. Pathological analysis of the lungs confirmed the diagnosis of a CCAM type II. The recent advent of the three‐dimensional ultrasound inversion mode opens many diagnostic options until now unavailable or difficult to attain sonographically and therefore it may help us to increase the sonographic detection rates of congenital cystic adenomatoid malformation.
Longitudinal follow up of delayed eclampsia using cerebroangiography
Authors: Negishi Yasuyuki , Otsubo Yasuo 
Published: September 2008

AbstractA 33‐year‐old primigravida experienced a delayed eclamptic convulsion. Following the convulsion, an i.v. administration of magnesium sulfate was utilized in response to cerebroarterial vasospasms, which were seen before and 1 week after the convulsion. Our findings confirm that the appropriate use of magnesium sulfate is necessary in such cases.
Triplet pregnancy with partial hydatidiform mole coexisting with two fetuses: A case report
Authors: Kim Cheol Hong , Kim Yoon Ha , Kim Jong Woon , Kim Ki Min , Cho Moon Kyoung , Kim Seok Mo , Nam Jong Hee , Song Tae‐Bok 
Published: September 2008

AbstractHydatidiform mole with a coexistent fetus is rare, but this condition has recently shown an increased incidence because of assisted reproduction technology. Herein, we report on a case of triplet pregnancy with a partial hydatidiform mole coexisting with two fetuses. It was diagnosed by p57kip2 immunohistochemical staining which is helpful in determining histologically equivocal cases. After termination of pregnancy, the patient was diagnosed with persistent gestational trophoblastic disease. Six courses of methotrexate chemotherapy were performed. Her β‐human chorionic gonadotrophin titers then fell to a normal level.
Efficacy of array comparative genomic hybridization in a fetus with an inherited apparently balanced translocation: A case report
Authors: Park Ji Kwon , Lee Jae Ik , Jo Hyun Cheol , Shin Jeong Kyu , Lee Soon Ae , Lee Jong Hak , Paik Won Young 
Published: September 2008

AbstractWhen one parent is discovered to have the same apparently balanced genetic rearrangement as that identified at prenatal diagnosis, many couples are advised to seek a more precise prenatal diagnosis for reassurance. A 25‐year‐old translocation carrier, who carried a fetus with partial trisomy 3q and partial monosomy 9p with omphalocele in a previous pregnancy, showed the same apparently balanced translocation at chorionic villus sampling. A truly balanced translocation without cryptic imbalances for the fetus was detected using array comparative genomic hybridization. For cryptic unbalanced defects, in which an apparently balanced translocation has been transmitted from a normal parent to a child with a phenotypic abnormality, the use of array comparative genomic hybridization to assess the presence of cryptic aberrations in the fetus combines the speed of DNA analysis with a comprehensive scan for submicroscopic genomic abnormalities.
Concurrent sigmoid volvulus and herniation through broad ligament defect during pregnancy: Case report and literature review
Authors: Vo Thi M. , Gyaneshwar Rajat , Mayer Christiane 
Published: September 2008

AbstractSigmoid volvulus in pregnancy is a rare complication. The combination of sigmoid volvulus and sigmoid herniation through a broad ligament together with placental abruption in pregnancy is a most unusual obstetrical emergency. We report the case of a previously well 32‐year‐old Mexican woman who was transferred to our institution at 28 weeks gestation with threatened preterm labor. We considered the possibility of appendicitis. While awaiting a surgical consult, an emergency cesarean section was carried out for a prolonged deceleration of the fetal heart. A routine transverse suprapubic abdominal entry revealed a large loop of ischemic bowel. This was confirmed to be a sigmoid volvulus which had herniated through a left broad ligament defect. The deceleration was attributed to a large placental abruption. No previous case with a combination of a sigmoid volvulus and placental abruption was found in the literature.
Adnexal torsion during pregnancy: Report of four cases and review of the literature
Authors: Hasiakos Dimitris , Papakonstantinou Katerina , Kontoravdis Antonios , Gogas Lazaros , Aravantinos Leon , Vitoratos Nikolaos 
Published: September 2008

AbstractAdnexal torsion is a rare cause of acute abdominal pain during pregnancy. It is frequently associated with ovarian stimulation for in vitro fertilization (IVF) or with ovarian masses, mainly of functional origin. The clinical, laboratory and imaging findings are non‐specific. The authors present four cases with adnexal torsion diagnosed during the first trimester of pregnancy. The clinical picture, the mode of diagnosis, and the therapeutic approach are discussed. In two cases, the adnexa was removed, because there was extensive hemorrhage and ischemia. In the other two cases, unwinding of the adnexa was carried out and the ovary was preserved. The diagnosis of adnexal torsion is difficult, especially during pregnancy, and occasionally remains a diagnostic dilemma. It necessitates a prompt surgical intervention, because any delay leads to irreversible ovarian necrosis, so that adnexectomy is ultimately required.