bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered plasma proteome during an early phase of peritonitis-induced sepsis
Authors: Thongboonkerd Visith , Chiangjong Wararat , Mares Jan , Moravec Jiri , Tuma Zdenek , Karvunidis Thomas , Sinchaikul Supachok , Chen Shui-Tein , Opatrný Karel , Matejovic Martin 
Published: November 2008

Sepsis is a systemic response to infection commonly found in critically ill patients and is associated with multi-organ failure and high mortality rate. Its pathophysiology and molecular mechanisms are complicated and remain poorly understood. In the present study, we performed a proteomics investigation to characterize early host responses to sepsis as determined by an altered plasma proteome in a porcine model of peritonitis-induced sepsis, which simulated several clinical characteristics of human sepsis syndrome. Haemodynamics, oxygen exchange, inflammatory responses, oxidative and nitrosative stress, and other laboratory parameters were closely monitored. Plasma samples were obtained from seven pigs before and 12 h after the induction of sepsis, and plasma proteins were resolved with two-dimensional gel electrophoresis (n=7 gels/group; before being compared with during sepsis). The resolved proteins were stained with the SYPRO Ruby fluorescence dye and subjected to quantitative and comparative analyses. From approx. 1500 protein spots visualized in each gel, levels of 36 protein spots were significantly altered in the plasma of animals with sepsis (sepsis/basal ratios or degrees of change ranged from 0.07 to 21.24). Q-TOF (quadrupole–time-of-flight) MS and MS/MS (tandem MS) identified 30 protein forms representing 22 unique proteins whose plasma levels were increased, whereas six forms of five unique proteins were significantly decreased during sepsis. The proteomic results could be related to the clinical features of this animal model, as most of these altered proteins have important roles in inflammatory responses and some of them play roles in oxidative and nitrosative stress. In conclusion, these findings may lead to a better understanding of the pathophysiology and molecular mechanisms underlying the sepsis syndrome.
First Report of Tilletia pulcherrima Bunt on Switchgrass (Panicum virgatum) in Texas
Authors: Carris L. M. , Castlebury L. A. , Zale J. 
Published: November 2008

Switchgrass seed samples of ‘Blackwell’ and ‘Alamo’ from Bailey County, TX were examined for bunt fungi. Fourteen completely bunted seeds of ‘Blackwell’ and four of ‘Alamo’ were found. No partially bunted seeds were found. Bunted seeds were darker and occasionally slightly swollen relative to noninfected seeds. Teliospores were globose to subglobose, 21 to 28 × 20 to 27 μm in diameter, dark reddish brown to nearly black, with blunt warts 1 to 1.8 μm long, enveloped in a hyaline sheath, and often with a short apiculus. Sterile cells were globose to subglobose, 17.5 to 22 μm, with smooth, laminated walls as much as 2.6 μm thick, and often with a short apiculus. This bunt was identified as Tilletia pulcherrima Ellis & Galloway on the basis of host and spore morphology (2). The internal transcribed spacer regions 1 and 2, including the 5.8S rDNA, were sequenced from bunted ‘Blackwell’ seeds (GenBank Accession No. EU915293, WSP 71501). The sequence was distinct from all Tilletia sequences in GenBank, including Tilletia barclayana (Bref.) Sacc. & Syd. on Panicum obtusum Kunth (GenBank Accession No. AF 310169) (1). To our knowledge, this is the first report of T. pulcherrima from switchgrass in Texas. Plant pathologists and regulatory officials should be aware of the potential for misidentification of T. pulcherrima as T. indica Mitra, the Karnal bunt pathogen of wheat that has similar spores, occurs in Texas, and has quarantine status. References: (1) R. Durán and G. W. Fischer. The Genus Tilletia, Washington State University, Pullman, WA, 1961. (2) K. Vánky, Mycotaxon 91:217, 2005.
First Report of Orobanche crenata on Sulla (Hedysarum coronarium) in Andalusia, Southern Spain
Authors: Córdoba E. , González-Verdejo C. I. , Die J. , Román B. , Nadal S. 
Published: November 2008

Crenata broomrape (Orobanche crenata Forsk.), an obligate chlorophyll-lacking root parasite, is the major constraint for growing legume crops in infested soils in southern Spain. Peas (Pisum sativum L), faba beans (Vicia faba L.), and narbon bean (Vicia narbonensis L.) are seriously affected (1,2,4). However, no information is available regarding its ability to attack sulla (Hedysarum coronarium L.), which is an important fodder legume grown in rain-fed conditions, yielding as much as 10,000 kg/ha of dry matter. In 2008, we found broomrape shoots infecting 4 to 5% of sulla plants in a trial field (two land races, P-26 and P-3, in a 500-m2 area) in Cordoba (Andalusia, southern Spain). The shoots developed numerous fertile flowering stalks throughout the field. The previous crop, narbon bean, was heavily infected during 2007 by O. crenata. Infection of sulla plants was confirmed by digging up the plant to verify the attachment of the broomrape plant to the sulla roots. The level of infection was low with only one to two emerged broomrape shoots per sulla plant infested. Morphology was typical of O. crenata. The calyx of the flowers was 13 to 18 mm long with free, bidentate segments. The white corolla of the flowers was 18 to 28 mm long and glandular pubescent. The lips were divergent, large, not ciliate, and filaments inserted 2 to 3 mm above the base of the corolla are hairy at base with glandular hair at the apex. The anthers were brown (3). Specimens were deposited in the Herbarium of the University of Córdoba (identification number COA 45358). To our knowledge, this first report of O. crenata parasitizing sulla shows that control methods for O. crenata will be needed if sulla is to be used as an alternative legume fodder in the infested soils of southern Spain. References: (1) J. I. Cubero and M. T. Moreno. Page 41 in: Some Current Research on Vicia faba in Western Europe. D. A. Bond et al., eds. Luxembourg, 1979. (2) S. Nadal et al. Plant Breed. 126:110, 2007. (3) A. J. Pujadas-Salvá. Page 187 in: Resistance to Orobanche: The State of the Art. Junta de Andalucía, Sevilla, Spain, 1999. (4) D. Rubiales et al. Crop Prot. 22:865, 2003.
First Report of White Mold Caused by Sclerotinia sclerotiorum on Borago officinalis in Italy
Authors: Garibaldi A. , Pensa P. , Bertetti D. , Gullino M. L. 
Published: November 2008

Borago officinalis L. (common borage) is a flowering plant belonging to the Boraginaceae with several culinary and medicinal uses and grown in soil or as potted plants in Liguria (northern Italy). At the end of winter in 2008, extensive chlorosis was observed on potted plants of B. officinalis grown in containers on a farm near Albenga (northern Italy). Initial symptoms included stem necrosis at the soil level and darkening of leaves. Disease was characterized by the presence of soft, watery tissues, particularly on affected leaves. As stem and foliar necrosis progressed, infected plants wilted and died. Wilt occurred within a few days on young plants. Symptomatic plants were found on 10 farms: average disease incidence in some nurseries reached 20%. Necrotic tissues became covered with a whitish mycelium that produced dark sclerotia. The diseased stem tissue was surface disinfected for 1 min in 1% NaOCl and plated on potato dextrose agar (PDA) amended with 100 ppm of streptomycin sulfate. Sclerotinia sclerotiorum (Lib.) de Bary (2) was consistently recovered from infected stem pieces. Sclerotia observed on infected plants at the soil level measured 2 to 9 × 1.5 to 7 (average 6 × 3) mm. Sclerotia produced on PDA measured 3 to 7 × 2 to 4 (average 4.4 × 3.1) mm. The internal transcribed spacer (ITS) region of rDNA was amplified using primers ITS4/ITS6 (3) and then sequenced. BLAST analysis of the 521-bp segment showed a 100% homology with the sequence of S. sclerotiorum. The nucleotide sequence has been assigned GenBank Accession No. EU627005. Pathogenicity of four isolates obtained from infected plants and used in mixture was confirmed by inoculating 10 30-day-old plants grown in 14-cm-diameter pots that were maintained in the greenhouse. Inoculum, consisting of 1-cm2 mycelial plugs excised from a 10-day-old PDA culture of each isolate, was placed on the soil surface around the base of each plant. Ten noninoculated plants served as a control. Plants were covered with a plastic bag for 4 days after inoculation. The inoculation trial was repeated once. All plants were kept at an average temperature of 20°C and watered as needed. All inoculated plants developed symptoms of leaf yellowing within 4 days, followed by the appearance of white mycelium and sclerotia and eventual wilt. Control plants remained symptomless. S. sclerotiorum was reisolated from the stems of inoculated plants. To our knowledge, this is the first report of white mold on B. officinalis in Italy as well as in Europe. The disease was recently reported in North Dakota (1). The economic importance of this disease is currently low; however, the request of borage for culinary uses is increasing. References: (1) C. A. Bradley et al. Plant Dis. 89:208, 2005. (2) N. F. Buchwald. Den. Kgl. Veterin.er-og Landbohojskoles Aarsskrift 5:1949. (3) D. E. I. Cooke and J. M. Duncan. Mycol. Res. 101:667, 1997.
Electroencephalographic Order Pattern Analysis for the Separation of Consciousness and Unconsciousness
Authors: Jordan Denis , Stockmanns Gudrun , Kochs Eberhard F. , Pilge Stefanie , Schneider Gerhard 
Published: November 2008

Background
Nonlinear electroencephalographic parameters, e.g., approximate entropy, have been suggested as measures of the hypnotic component of anesthesia. Compared with linear methods, they may detect additional information and quantify the irregularity of a dynamical system. High dimensionality of a signal and disturbances may affect these parameters and change their ability to distinguish consciousness from unconsciousness. Methods of order pattern analysis, in this investigation represented by permutation entropy, recurrence rate, and phase coupling of order recurrence plots, are suitable for any type of time series, whether deterministic or noisy. They may provide a better estimation of the hypnotic component of anesthesia than other nonlinear parameters.

Methods
The current analysis is based on electroencephalographic data from two similar clinical studies in adult patients undergoing general anesthesia with sevoflurane or propofol. The study period was from induction until patients followed command after surgery, including a reduction of the hypnotic agent after tracheal intubation until patients followed command. Prediction probability was calculated to assess the parameter's ability to separate consciousness from unconsciousness at the transition between both states.

Results
Parameters of order pattern analysis provide a prediction probability of maximal 0.85 (training study) and 0.78 (evaluation study) with frequencies from 0 to 30 Hz, and maximal 0.87 (training study) and 0.83 (evaluation study) including frequencies up to 70 Hz, both higher than 0.77 (approximate entropy).

Conclusions
Parameters of the nonlinear method order pattern analysis separate consciousness from unconsciousness and are grossly independent of high-frequency components of the electroencephalogram.
Sex Differences in the Effect of Dyspnea on Thermal Pain Threshold in Young Healthy Subjects
Authors: Nishino Takashi , Isono Shiroh , Ishikawa Teruhiko , Shinozuka Norihiro 
Published: November 2008

Background
Previous study has demonstrated that dyspnea exerts inhibitory influence on pain, and empirical research supports the existence of sex differences in pain. To test the hypothesis that the inhibitory influence of dyspnea on the pain sensation may be less in females than in males, the authors investigated the sex differences in the responses of thermal pain threshold to dyspnea in healthy young subjects.

Methods
The authors measured changes in thermal pain threshold in 30 female subjects and 30 male subjects before and during dyspnea produced by a combination of hypercapnia and elastic loading, and compared the difference between males and females.

Results
The thermal pain threshold significantly increased during loaded breathing in male subjects (46.0 degrees +/- 1.3 degrees vs. 47.2 degrees +/- 1.2 degrees C; P < 0.01, baseline vs. loaded breathing), whereas no change was observed in female subjects (46.1 degrees +/- 1.3 degrees vs. 46.0 degrees +/- 1.4 degrees C; P > 0.1). No significant correlation was observed between the values of dyspneic visual analog scale and changes in thermal pain threshold. Comparison of the different phases of the menstrual cycle in female subjects also showed that there was no consistent effect of the particular phase on thermal pain threshold (45.7 degrees +/- 1.0 degrees vs. 46.1 degrees +/- 1.4 degrees C; P > 0.1, follicular phase vs. luteal phase during baseline; and 45.9 degrees +/- 1.1 degrees vs. 46.0 degrees +/- 1.7 degrees C; P > 0.1, follicular phase vs. luteal phase during loaded breathing).

Conclusion
The inhibitory influence of dyspnea on the pain sensation is less in females than in males, but the sex difference may not be explained by female reproductive hormones alone.
Gabapentin Acts within the Locus Coeruleus to Alleviate Neuropathic Pain
Authors: Hayashida Ken-ichiro , Obata Hideaki , Nakajima Kunie , Eisenach James C. 
Published: November 2008

Background
Gabapentin recruits descending inhibition to produce analgesia after nerve injury, but whether this is a local action in the brainstem is unknown. The authors hypothesized that gabapentin activates noradrenergic neurons in the locus coeruleus (LC) by a local action.

Methods
Male rats underwent L5-L6 spinal nerve ligation (SNL) and received drugs by intra-LC or systemic routes for behavior testing, immunohistochemistry in the LC, and microdialysis in the spinal dorsal horn. In other studies, brainstem slices from normal and SNL animals were used for immunohistochemistry.

Results
SNL increased phosphorylated cyclic adenosine monophosphate response element binding protein (pCREB)-expressing nuclei bilaterally in the LC, and increased noradrenaline release in the spinal dorsal horn. Gabapentin, whether in isolated brainstem slices or in conscious or anesthetized animals, increased pCREB-expressing nuclei in the LC. The net increase in pCREB expression by gabapentin did not differ between normal and SNL conditions. This gabapentin-induced pCREB activation in LC neurons was abolished by an AMPA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Intra-LC-injected gabapentin reduced hypersensitivity in SNL rats in a dose-dependent manner. Both intra-LC coadministration of CNQX and intrathecal administration of the alpha2-adrenoceptor antagonist idazoxan blocked antihypersensitivity by intra-LC gabapentin. Intravenous gabapentin induced noradrenaline release in the spinal dorsal horn. The net amount of noradrenaline release by gabapentin is larger in SNL rats compared with the normal condition, although the percentage increases from the baseline were the same.

Conclusions
These results suggest that gabapentin acts directly in the brainstem via a glutamate-dependent mechanism to stimulate descending inhibition to produce antihypersensitivity after peripheral nerve injury.
γ-Aminobutyric Acid–mediated Neurotransmission in the Pontine Reticular Formation Modulates Hypnosis, Immobility, and Breathing during Isoflurane Anesthesia
Authors: Vanini Giancarlo , Watson Christopher J. , Lydic Ralph , Baghdoyan Helen A. 
Published: November 2008

Background
Many general anesthetics are thought to produce a loss of wakefulness, in part, by enhancing gamma-aminobutyric acid (GABA) neurotransmission. However, GABAergic neurotransmission in the pontine reticular formation promotes wakefulness. This study tested the hypotheses that (1) relative to wakefulness, isoflurane decreases GABA levels in the pontine reticular formation; and (2) pontine reticular formation administration of drugs that increase or decrease GABA levels increases or decreases, respectively, isoflurane induction time.

Methods
To test hypothesis 1, cats (n = 5) received a craniotomy and permanent electrodes for recording the electroencephalogram and electromyogram. Dialysis samples were collected from the pontine reticular formation during isoflurane anesthesia and wakefulness. GABA levels were quantified using high-performance liquid chromatography. For hypothesis 2, rats (n = 10) were implanted with a guide cannula aimed for the pontine reticular formation. Each rat received microinjections of Ringer's (vehicle control), the GABA uptake inhibitor nipecotic acid, and the GABA synthesis inhibitor 3-mercaptopropionic acid. Rats were then anesthetized with isoflurane, and induction time was quantified as loss of righting reflex. Breathing rate was also measured.

Results
Relative to wakefulness, GABA levels were significantly decreased by isoflurane. Increased power in the electroencephalogram and decreased activity in the electromyogram caused by isoflurane covaried with pontine reticular formation GABA levels. Nipecotic acid and 3-mercaptopropionic acid significantly increased and decreased, respectively, isoflurane induction time. Nipecotic acid also increased breathing rate.

Conclusion
Decreasing pontine reticular formation GABA levels comprises one mechanism by which isoflurane causes loss of consciousness, altered cortical excitability, muscular hypotonia, and decreased respiratory rate.
Differential Response of Gray Poplar Leaves and Roots Underpins Stress Adaptation during Hypoxia
Authors: Kreuzwieser Jürgen , Hauberg Jost , Howell Katharine A. , Carroll Adam , Rennenberg Heinz , Millar A. Harvey , Whelan James 
Published: November 2008

AbstractThe molecular and physiological responses of gray poplar (Populus × canescens) following root hypoxia were studied in roots and leaves using transcript and metabolite profiling. The results indicate that there were changes in metabolite levels in both organs, but changes in transcript abundance were restricted to the roots. In roots, starch and sucrose degradation were altered under hypoxia, and concurrently, the availability of carbohydrates was enhanced, concomitant with depletion of sucrose from leaves and elevation of sucrose in the phloem. Consistent with the above, glycolytic flux and ethanolic fermentation were stimulated in roots but not in leaves. Various messenger RNAs encoding components of biosynthetic pathways such as secondary cell wall formation (i.e. cellulose and lignin biosynthesis) and other energy-demanding processes such as transport of nutrients were significantly down-regulated in roots but not in leaves. The reduction of biosynthesis was unexpected, as shoot growth was not affected by root hypoxia, suggesting that the up-regulation of glycolysis yields sufficient energy to maintain growth. Besides carbon metabolism, nitrogen metabolism was severely affected in roots, as seen from numerous changes in the transcriptome and the metabolome related to nitrogen uptake, nitrogen assimilation, and amino acid metabolism. The coordinated physiological and molecular responses in leaves and roots, coupled with the transport of metabolites, reveal important stress adaptations to ensure survival during long periods of root hypoxia.
A Relaxed Specificity in Interchain Disulfide Bond Formation Characterizes the Assembly of a Low-Molecular-Weight Glutenin Subunit in the Endoplasmic Reticulum
Authors: Lombardi Alessio , Barbante Alessandra , Cristina Pietro Della , Rosiello Daniele , Castellazzi Chiara Lara , Sbano Luca , Masci Stefania , Ceriotti Aldo 
Published: November 2008

Abstract
Wheat (Triticum spp.) grains contain large protein polymers constituted by two main classes of polypeptides: the high-molecular-weight glutenin subunits and the low-molecular-weight glutenin subunits (LMW-GS). These polymers are among the largest protein molecules known in nature and are the main determinants of the superior technological properties of wheat flours. However, little is known about the mechanisms controlling the assembly of the different subunits and the way they are arranged in the final polymer. Here, we have addressed these issues by analyzing the formation of interchain disulfide bonds between identical and different LMW-GS and by studying the assembly of mutants lacking individual intrachain disulfides. Our results indicate that individual cysteine residues that remain available for disulfide bond formation in the folded monomer can form interchain disulfide bonds with a variety of different cysteine residues present in a companion subunit. These results imply that the coordinated expression of many different LMW-GS in wheat endosperm cells can potentially lead to the formation of a large set of distinct polymeric structures, in which subunits can be arranged in different configurations. In addition, we show that not all intrachain disulfide bonds are necessary for the generation of an assembly-competent structure and that the retention of a LMW-GS in the early secretory pathway is not dependent on polymer formation.
Genetic and Molecular Characterization of theVRN2Loci in Tetraploid Wheat
Authors: Distelfeld Assaf , Tranquilli Gabriela , Li Chengxia , Yan Liuling , Dubcovsky Jorge 
Published: November 2008

AbstractWinter wheat (Triticum spp.) varieties require long exposures to low temperatures to flower, a process called vernalization. The VRN2 locus includes two completely linked zinc finger-CCT domain genes (ZCCT1 and ZCCT2) that act as flowering repressors down-regulated during vernalization. Deletions or mutations in these two genes result in the elimination of the vernalization requirement in diploid wheat (Triticum monococcum). However, natural allelic variation in these genes has not been described so far in polyploid wheat (tetraploid Triticum turgidum and hexaploid Triticum aestivum). A tetraploid wheat population segregating for both VRN-A2 and VRN-B2 loci facilitated the characterization of different alleles. Comparisons between functional and nonfunctional alleles revealed that both ZCCT1 and ZCCT2 genes are able to confer vernalization requirement and that different ZCCT genes are functional in different genomes. ZCCT1 and ZCCT2 proteins from nonfunctional vrn2 alleles have mutations at arginine amino acids at position 16, 35, or 39 of the CCT domain. These positions are conserved between CCT and HEME ACTIVATOR PROTEIN2 (HAP2) proteins, supporting a model in which the action of CCT domains is mediated by their interactions with HAP2/HAP3/HAP5 complexes. This study also revealed natural variation in gene copy number, including a duplication of the functional ZCCT-B2 gene and deletions or duplications of the complete VRN-B2 locus. Allelic variation at the VRN-B2 locus was associated with a partially dominant effect, which suggests that variation in the number of functional ZCCT genes can be used to expand allelic diversity for heading time in polyploid wheat and, hopefully, improve its adaptation to different environments.