bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dangerous halo after neoadjuvant chemotherapy and two-step hepatectomy for colorectal liver metastases
Authors: Mentha G , Terraz S , Morel P , Andres A , Giostra E , Roth A , Rubbia-Brandt L , Majno P 
Published: December 2008

Abstract

Background
Bilobar colorectal metastases are a therapeutic challenge and require a multidisciplinary approach. The aim of this study was to describe the clinical and histological outcomes of patients having neoadjuvant chemotherapy and two-step hepatectomy with right portal vein occlusion for advanced bilateral colorectal metastases.

Methods
A series of 23 consecutive patients treated with curative intent according to a standardized multidisciplinary management protocol was reviewed.

Results
Of 23 patients, 22 completed the programme. There was no mortality and no Clavien grade III morbidity. Median survival from the start of treatment was 45 months, and 1-, 3- and 5-year Kaplan–Meier estimates were 95, 73 and 27 per cent respectively. On histology at the first operation, ten patients had a dangerous halo of proliferating tumour cells infiltrating the surrounding liver parenchyma, of variable importance (six focal and four diffuse), regardless of the response to chemotherapy of the metastases. The dangerous halo increased in prevalence and importance (six focal and seven diffuse) between the first and second operation.

Conclusion
Neoadjuvant chemotherapy followed by two-step hepatectomy with right portal vein occlusion is feasible, safe and may be advantageous to the patient. The appearance of a dangerous halo around the liver metastases may require adaptation of the surgical technique to decrease the risk of local recurrence.
Prognostic significance of insulin-like growth factor II mRNA-binding protein 3 expression in gastric adenocarcinoma
Authors: Jeng Y-M , Wang T-H , Lu S-H , Yuan R-H , Hsu H-C 
Published: December 2008

Abstract

Background
Insulin-like growth factor II mRNA-binding protein (IMP) 3 is expressed in embryonic tissues and multiple cancers. The aim was to establish the prognostic value of IMP-3 expression in gastric adenocarcinoma.

Methods
IMP-3 expression in resected gastric adenocarcinomas was analysed by immunohistochemistry.

Results
IMP-3 was expressed in 183 (58·1 per cent) of 315 tumours. Expression was associated with older age (P &lt; 0·001), larger tumour size (P = 0·009), deep tumour invasion (P &lt; 0·001) and lymph node metastasis (P &lt; 0·001). IMP-3-positive tumours were associated with poorer 5-year survival than negative tumours at all stages (stage I, 82 versus 97 per cent; stage II, 55 versus 78 per cent; stage III and IV, 11 versus 25 per cent; P = 0·005, P = 0·033 and P = 0·036 respectively). Multivariable analysis identified IMP-3 (hazard ratio (HR) 1·93), depth of tumour invasion (HR 3·69, 9·77 and 10·69 for pathological tumour stage (pT) 2, pT3 and pT4 respectively versus pT1), and lymph node metastasis (HR 1·57, 3·29 and 3·40 for pathological node stage (pN) 1, pN2 and pN3 respectively versus pN0) as independent prognostic factors.

Conclusion
IMP-3 expression correlates with the metastatic potential of gastric adenocarcinoma and is an independent prognostic factor.
Testing LED lighting for colour discrimination and colour rendering
Authors: Mahler Elodie , Ezrati Jean‐Jacques , Viénot Françoise 
Published: December 2008

AbstractLight‐emitting diode (LED) technology offers the possibility of obtaining white light, despite narrow‐band spectra. In order to characterize the colour discrimination efficiency of various LED clusters, we designed a classification test, composed of 32 caps equally distributed along the hue circle at about 3 ΔE* ab‐unit intervals. Forty normal colour observers were screened under four different LED test light sources adjusted for best colour rendering, and under one control incandescent light of the same colour temperature. We used commercially available red, green, blue, and/or amber LED clusters. These yielded a poor colour rendering index (CRI). They also induced a significantly higher number of erroneous arrangements than did the control light. Errors are located around greenish‐blue and purplish‐red shades, parallel to the yellow‐axis direction, whereas when the distribution of light covers the full spectrum, the LED clusters achieve satisfactory colour discrimination efficiency. With respect to the lights we tested, the colour discrimination is correlated with the CIE CRIs as well as with a CRI based on our sample colours. We stress the fact that increasing the chroma of samples by lighting does not necessarily imply an improvement of colour discrimination. © 2008 Wiley Periodicals, Inc. Col Res Appl, 34, 8–17, 2009.
Cinematic‐film protection using metameric blacks
Authors: Rastar Amir 
Published: December 2008

AbstractThis article presents a new approach for security management in digital cinema applications. Metameric blacks are used as a new try for cinematic‐film protection procedures, which are used in theatre projection systems. The information given by the capturing device in production time decomposes to its base spectra and makes an unacceptable image quality for camcorders by adding a specific metameric black to it. This procedure takes place during the exhibition, and the camcorders take poor quality pictures although the image may not have any changes in its quality for the audience. This try tends to reach the goal of security management during the movie plays and may be a new method to keep the rights of producers against digital theft. © 2008 Wiley Periodicals, Inc. Col Res Appl, 34, 93–96, 2009.
Proposal for an indoor daylight illuminant
Authors: Gombos Katalin , Pointer Michael , Sik‐Lányi Cecília , Schanda János , Tarczali Tünde 
Published: December 2008

AbstractTwo indoor daylight illuminants are recommended for general colorimetric and graphic arts use. The daylight illuminants are derived using CIE standard illuminant D65 and illuminant D50 together with the average spectral transmission of a large number of window glass samples. We show that the average window glass filters not only the blue part of the spectrum but also the red part to such an extent that it has to be taken into consideration. Apart from some extreme glass types, the bulk of the window glasses now in use do not show a very large variation in their spectral transmittances, so that an average curve can adequately represent their filtering action. © 2008 Wiley Periodicals, Inc. Col Res Appl, 34, 18–25, 2009.
An evaluator's balancing act: Making decisions about methodological rigor
Authors: Braverman Marc T. , Arnold Mary E. 
Published: December 2008

AbstractMethodological rigor consists of a series of elements that, in combination, determine the confidence with which conclusions can be drawn from the evaluation results. These elements include evaluation design, conceptualization of constructs, measurement strategies, time frames, program integrity, and others. The authors examine the factors that influence rigor‐related evaluation planning decisions and the relationship of rigor to evaluation use by the sponsoring organization. Rather than following generalized predetermined standards, decisions about rigor should be based on the specific organizational context, information needs for the evaluation, and anticipated benefits and costs of available methodological alternatives. Extension offers a rich organizational context for discussion because of the diversity and complexity of its funding, stakeholders, programs, and evaluations. Several roles are proposed through which evaluators can promote organizational learning with regard to the contribution of methodological rigor to sound evaluation practice. Two Extension evaluations are presented as examples; though they reflect differing levels of rigor, both were thoughtfully planned and resulted in strong use of findings. © Wiley Periodicals, Inc.
The value of evaluation in cooperative extension
Authors: Duttweiler Michael W. 
Published: December 2008

AbstractThe author considers whether current evaluation practice is of value to the Cooperative Extension System. Evaluation use and influence in Extension are characterized through literature review and examination of case examples informed by correspondence with study authors. The more than 675 evaluations examined consistently yielded specific and valuable program counsel applicable to Extension education practice. Nearly seven in ten studies addressed at least one aspect of program improvement. Beyond simply generating a supply of potentially useful findings, there was clear evidence of substantive program modification in response to program evaluation. Case examples indicated that evaluation studies have influenced Extension practice by helping to establish program direction, improving existing educational practice, informing public policy, establishing or sustaining program support, establishing a basis for resource allocation decisions, influencing relationships with stakeholders, and strengthening evaluation practice itself. The author concludes that evaluation practice is both dynamic and influential within the Cooperative Extension System. © Wiley Periodicals, Inc.
sup wit eval ext?
Authors: Patton Michael Quinn 
Published: December 2008

AbstractExtension and evaluation share some similar challenges, including working with diverse stakeholders, parallel processes for focusing priorities, meeting common standards of excellence, and adapting to globalization, new technologies, and changing times. Evaluations of extension programs have helped clarify how change occurs, especially the relationship between knowledge change and behavior change, the importance of systems thinking, the purposes of evaluation (accountability versus improvement versus knowledge generation), the significance of process use, and the challenge of matching methods to evaluation questions, resources, and purposes to ensure methodological appropriateness. © Wiley Periodicals, Inc.
White‐matter lesions along the cholinergic tracts are related to cortical sources of EEG rhythms in amnesic mild cognitive impairment
Authors: Babiloni Claudio , Pievani Michela , Vecchio Fabrizio , Geroldi Cristina , Eusebi Fabrizio , Fracassi Claudia , Fletcher Evan , De Carli Charles , Boccardi Marina , Rossini Paolo Maria , Frisoni Giovanni B. 
Published: December 2008

AbstractDoes impairment of cholinergic systems represent an important factor in the development of amnesic mild cognitive impairment (aMCI), as a preclinical stage of Alzheimer's disease (AD)? Here we tested the hypothesis that electroencephalographic (EEG) rhythms, known to be modulated by the cholinergic system, may be particularly affected in aMCI patients with lesions along the cholinergic white‐matter tracts. Eyes‐closed resting EEG data were recorded in 28 healthy elderly (Nold) and 57 aMCI patients. Lesions along the cholinergic white‐matter tracts were detected with fluid‐attenuated inversion recovery sequences on magnetic resonance imaging. The estimation of the cholinergic lesion was performed with a validated semi‐automatic algorithm pipeline after registration to a stereotactic template, image integration with stereotactic masks of the cholinergic tracts, and normalization to intracranial volume. The aMCI patients were divided into two groups of high (MCI Ch+; N = 29; MMSE = 26.2) and low cholinergic damage (MCI Ch−; N = 28; MMSE = 26.6). EEG rhythms of interest were delta (2–4 Hz), theta (4–8 Hz), alpha 1 (8–10.5 Hz), alpha 2 (10.5–13 Hz), beta 1 (13–20 Hz), and beta 2 (20–30 Hz). Cortical EEG generators were estimated by LORETA software. As main results, (i) power of occipital, parietal, temporal, and limbic alpha 1 sources was maximum in Nold, intermediate in MCI Ch−, and low in MCI Ch+ patients; (ii) the same trend was true in theta sources. These results are consistent with the hypothesis that damage to the cholinergic system is associated with alterations of EEG sources in aMCI subjects. Hum Brain Mapp 2009. © 2008 Wiley‐Liss, Inc.
Mutation in the DNA‐binding domain of the EWS‐Oct‐4 oncogene results in dominant negative activity that interferes with EWS‐Oct‐4‐mediated transactivation
Authors: Kim Sol , Lee Jungwoon , Kim Ja Young , Lim Bobae , Shin Eung‐Kyun , Han Yong‐Mahn , Kim Sung‐Su , Song Jin‐Ho , Kim Jungho 
Published: December 2008

AbstractThe EWS‐Oct‐4 protein is a chimeric molecule in which the amino terminal domain (NTD) of the EWS becomes fused to the carboxy terminal domain (CTD) of the Oct‐4 transcription factor. It was identified in human bone and soft‐tissue tumors associated with t(6;22)(p21;q12). Using in vitro and in vivo systems, we found that the EWS‐Oct‐4 protein self‐associates. The major domains required for self‐association mapped to the EWS NTD (amino acids 70–163) and the POU DNA‐binding domain. EWS‐Oct‐4 protein also associated with EWS‐Oct‐4 (V351P), which contains a mutation in the POU DNA‐binding domain. Using electrophoretic mobility shift assays, we found that the EWS‐Oct‐4 (V351P) mutant interfered with wild‐type EWS‐Oct‐4 DNA‐binding activity. In addition, we found that EWS‐Oct‐4‐mediated transcriptional activation was inhibited by EWS‐Oct‐4 (V351P) protein in vivo. Thus, this mutation in the POU DNA‐binding domain results in a dominant negative protein. These findings suggest that the biological functions of the EWS‐Oct‐4 oncogene can be modulated by the dominant negative mutant EWS‐Oct‐4 (V351P). © 2008 Wiley‐Liss, Inc.
Actin and Vimentin proteins with N‐terminal deletion detected in tumor‐bearing rat livers induced by intraportal‐vein injection of Ha‐ras‐transfected rat liver cells
Authors: Nakamura Yasushi , Kominami Akari , Tsujimoto Yoshiyuki , Nakayama Yuko , Kitahashi Tsukasa , Yoshimoto Sonoko , Kubo Asuka , Watanabe Shinpei , Kageyama Minami , Yokoyama Meiko , Kido Yasuhiro , Kobayashi Yukiko , Kuwahata Masashi , Chang Chia‐Cheng , Upham Brad L. , Trosko James E. , Park Eun Young , Sato Kenji 
Published: December 2008

AbstractThe introduction of the tumorigenic v‐Ha‐ras oncogene‐transformed rat liver epithelial cells (WBras), which is deficient in gap junctional intercellular communication (GJIC), into F344 rats, induces significant formation of hepatocellular tumors. GJIC plays a major role in maintaining tissue homeostasis. Using this in vivo tumor model system, we used 2‐dimensional electrophoresis with isoelectric focusing in the first dimension and SDS‐PAGE in the second dimension to globally identify proteins that are uniquely expressed in the livers of WBras‐treated rats as compared to the sham control. Immunoblotting was used to identify Ras and Connexin43, which were the positive and negative marker proteins, respectively, of the introduced WBras cells. As predicted, immunoblotting indicated that the whole liver of tumor‐bearing animals exhibited a decreased level of Connexin43 and an increased level of Ras. Connexin43 and GJIC were expressed and functional in normal liver, but not in the tumor. In addition to these 2 markers, an additional 4 proteins exhibited decreased levels and 2 proteins exhibited increased levels in the livers of tumor‐bearing animals. N‐Terminal sequencing analysis was used to identify these proteins, which were glucose‐regulated protein 78, 2 isoforms of heat shock protein 60, and the β‐chain of ATP synthase for the down regulated proteins, and β‐Actin with a 46 amino acid deletion from its N‐terminus and Vimentin with a 71 amino acid deletion from its N‐terminus for the up regulated proteins. These data offer potentially new markers of liver tumorigenicity, particularly, Vimentin. © 2008 Wiley‐Liss, Inc.
Spleen tyrosine kinase as a novel candidate tumor suppressor gene for human oral squamous cell carcinoma
Authors: Ogane Satoru , Onda Takeshi , Takano Nobuo , Yajima Tetsuo , Uchiyama Takeshi , Shibahara Takahiko 
Published: December 2008

AbstractWe analyzed the mutational and methylation status of the spleen tyrosine kinase (Syk) gene and both mRNA and protein levels in primary oral squamous cell carcinoma (OSCC) and OSCC‐derived cell lines and examined the function of the Syk gene in OSCC‐derived cell lines in vitro. Using quantitative real‐time reverse transcription polymerase chain reaction, Western blotting and immunofluorescence on 7 OSCC‐derived cell lines and normal oral keratinocytes (NOKs), Syk mRNA and protein expression were commonly downregulated in all cell lines compared to the NOKs. Although no sequence variation in the coding region of the Syk gene was identified in these cell lines, we found frequent hypermethylation in the CpG island region. Syk expression was restored by experimental demethylation. In addition, using a wound healing assay and in vitro invasion assay, we performed functional analysis using Syk transfected into the OSCC‐derived cell lines, and they showed significant inhibition of motility and invasiveness. In clinical samples, high frequencies of Syk downregulation were detected by immunohistochemistry (33 of 53 [62%]). Furthermore, the Syk expression status was correlated significantly (p = 0.047) with tumor metastasis to cervical lymph nodes. These results suggest that the Syk gene is frequently inactivated during oral carcinogenesis and that an epigenetic mechanism may regulate loss of expression possibly leading to metastasis. © 2008 Wiley‐Liss, Inc.
Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein
Authors: Manuelidis Laura , Liu Ying , Mullins Brian 
Published: December 2008

AbstractHuman CJD, endemic sheep scrapie, epidemic bovine spongiform encephalopathy (BSE), and other transmissible spongiform encephalopathies (TSEs), are caused by a group of related but molecularly uncharacterized infectious agents. The UK‐BSE agent infected many species, including humans where it causes variant CJD (vCJD). As in most viral infections, different TSE disease phenotypes are determined by both the agent strain and the host species. TSE strains are most reliably classified by incubation time and regional neuropathology in mice expressing wild‐type (wt) prion protein (PrP). We compared vCJD to other human and animal derived TSE strains in both mice and neuronal cultures expressing wt murine PrP. Primary and serial passages of the human vCJD agent, as well as the highly selected mutant 263K sheep scrapie agent, revealed profound strain‐specific characteristics were encoded by the agent, not by host PrP. Prion theory posits that PrP converts itself into the infectious agent, and thus short incubations require identical PrP sequences in the donor and recipient host. However, wt PrP mice injected with human vCJD brain homogenates showed dramatically shorter primary incubation times than mice expressing only human PrP, a finding not in accord with a PrP species barrier. All mouse passage brains showed the vCJD agent derived from a stable BSE strain. Additionally, both vCJD brain and monotypic neuronal cultures produced a diagnostic 19 kDa PrP fragment previously observed only in BSE and vCJD primate brains. Monotypic cultures can be used to identify the intrinsic, strain‐determining molecules of TSE infectious particles. J. Cell. Biochem. 106: 220–231, 2009. © 2008 Wiley‐Liss, Inc.
Aberrant regulation and modification of heat shock factor 1 in senescent human diploid fibroblasts
Authors: Lee Yoon Kwang , Liu Diana J. , Lu Jiebo , Chen Kuang Yu , Liu Alice Y.‐C. 
Published: December 2008

AbstractInduction of the heat shock response (HSR), determined by hsp70‐luciferase reporter and HSP70 protein expression, is attenuated as a function of age of the IMR‐90 human diploid fibroblasts. To better understand the underlying mechanism, we evaluated changes in the regulation and function of the HSF1 transcription factor. We show that the activation of HSF1 both in vivo and in vitro was decreased as a function of age, and this was attributable to a change in the regulation of HSF1 as the abundance of HSF1 protein and mRNA was unaffected. HSF1 was primarily cytosolic in young cells maintained at 37°C, and heat shock promoted its quantitative nuclear translocation and trimerization. In old cells, some HSF1 was nuclear sequestered at 37°C, and heat shock failed to promote the quantitative trimerization of HSF1. These changes in HSF1 could be reproduced by treating young cells with H2O2 to stunt them into premature senescence. Flow cytometry measurement of peroxide content showed higher levels in old cells and H2O2‐induced premature senescent cells as compared to young cells. Experiments using isoelectric focusing and Western blot showed age‐dependent changes in the mobility of HSF1 in a pattern consistent with its S‐glutathiolation and S‐nitrosylation; these changes could be mimicked by treating young cells with H2O2. Our results demonstrated dynamic age‐dependent changes in the regulation but not the amount of HSF1. These changes are likely mediated by oxidative events that promote reversible and irreversible modification of HSF1 including S‐glutathiolation and S‐nitrosylation. J. Cell. Biochem. 106: 267–278, 2009. © 2008 Wiley‐Liss, Inc.
Effects of MAPK signaling on 1,25‐dihydroxyvitamin D‐mediated CYP24 gene expression in the enterocyte‐like cell line, Caco‐2
Authors: Cui Min , Zhao Yan , Hance Kenneth W. , Shao Andrew , Wood Richard J. , Fleet James C. 
Published: December 2008

AbstractWe examined the role of the extracellular signal regulated kinases (ERK) in 1,25‐dihydroxyvitamin D (1,25(OH)2D3)‐induced gene expression in the differentiated Caco‐2 cells. 1,25(OH)2D3‐regulated expression of the 25‐hydroxyvitamin D, 24‐hydroxylase (CYP24) gene (both natural gene and promoter construct) was strongly modulated by altering ERK activity (i.e., reduced by MEK inhibitors and dominant negative (dn) ERK1 and ERK2, activated by epidermal growth factor) but ERK inhibition had no effect on 1,25(OH)2D3‐regulated expression of the transient receptor potential cation channel, subfamily V, member 6 (TRPV6). ERK5‐mediated phosphorylation of the transcription factor Ets‐1 enhanced 1,25(OH)2D3‐mediated CYP24 gene transcription in proliferating but not differentiated Caco‐2 cells due to reduced levels of ERK5 and Ets‐1 (total and phosphoprotein levels) in differentiated cells. MEK inhibition reduced 1,25(OH)2D3‐induced 3X‐VDRE promoter activity but had no impact on the association of vitamin D receptor (VDR) with chromatin suggesting a role for co‐activator recruitment in ERK‐modulation of vitamin D‐regulated CYP24 gene activation. Chromatin immunoprecipitation assays revealed that the ERK1/2 target, mediator 1 (MED1), is recruited to the CYP24, but not the TRPV6, promoter following 1,25(OH)2D3 treatment. MED1 phosphorylation was sensitive to activators and inhibitors of the ERK1/2 signaling and MED1 siRNA reduced 1,25(OH)2D3‐regulated human CYP24 promoter activity. This suggests ERK1/2 signaling enhances 1,25(OH)2D3 effects on the CYP24 promoter by MED1‐mediated events. Our data show that there are both promoter‐specific and cell stage‐specific roles for the ERK signaling pathway on 1,25(OH)2D3‐mediated gene induction in enterocyte‐like Caco‐2 cells. J. Cell. Physiol. 219: 132–142, 2009. © 2008 Wiley‐Liss, Inc.