bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell wall
Authors: de Haan Diede , Ramos Nahuel‐Hernán , Meng Yu‐Feng , Rotkopf Ron , Addadi Yoseph , Rosenhek‐Goldian Irit , Gal Assaf 
Published: April 2024

Summary

Unicellular organisms are known to exert tight control over their cell size. In the case of diatoms, abundant eukaryotic microalgae, two opposing notions are widely accepted. On the one hand, the rigid silica cell wall that forms inside the parental cell is thought to enforce geometrical reduction of the cell size. On the other hand, numerous exceptions cast doubt on the generality of this model.

Here, we monitored clonal cultures of the diatom Stephanopyxis turris for up to 2 yr, recording the sizes of thousands of cells, in order to follow the distribution of cell sizes in the population.

Our results show that S. turris cultures above a certain size threshold undergo a gradual size reduction, in accordance with the postulated geometrical driving force. However, once the cell size reaches a lower threshold, it fluctuates around a constant size using the inherent elasticity of cell wall elements.

These results reconcile the disparate observations on cell size regulation in diatoms by showing two distinct behaviors, reduction and homeostasis. The geometrical size reduction is the dominant driving force for large cells, but smaller cells have the flexibility to re‐adjust the size of their new cell walls.
Guard cell and subsidiary cell sizes are key determinants for stomatal kinetics and drought adaptation in cereal crops
Authors: Rui Mengmeng , Chen Rongjia , Jing Yi , Wu Feibo , Chen Zhong‐Hua , Tissue David , Jiang Hangjin , Wang Yizhou 
Published: April 2024

Summary
Climate change‐induced drought is a major threat to agriculture. C4 crops have a higher water use efficiency (WUE) and better adaptability to drought than C3 crops due to their smaller stomatal morphology and faster response. However, our understanding of stomatal behaviours in both C3 and C4 Poaceae crops is limited by knowledge gaps in physical traits of guard cell (GC) and subsidiary cell (SC).
We employed infrared gas exchange analysis and a stomatal assay to explore the relationship between GC/SC sizes and stomatal kinetics across diverse drought conditions in two C3 (wheat and barley) and three C4 (maize, sorghum and foxtail millet) upland Poaceae crops. Through statistical analyses, we proposed a GCSC‐τ model to demonstrate how morphological differences affect stomatal kinetics in C4 Poaceae crops.
Our findings reveal that morphological variations specifically correlate with stomatal kinetics in C4 Poaceae crops, but not in C3 ones. Subsequent modelling and experimental validation provide further evidence that GC/SC sizes significantly impact stomatal kinetics, which affects stomatal responses to different drought conditions and thereby WUE in C4 Poaceae crops.
These findings emphasize the crucial advantage of GC/SC morphological characteristics and stomatal kinetics for the drought adaptability of C4 Poaceae crops, highlighting their potential as future climate‐resilient crops.
Quantifying the production of plant pollen at the farm scale
Authors: Wright Ellen K. , Timberlake Thomas P. , Baude Mathilde , Vaughan Ian P. , Memmott Jane 
Published: April 2024

Summary
Plant pollen is rich in protein, sterols and lipids, providing crucial nutrition for many pollinators. However, we know very little about the quantity, quality and timing of pollen availability in real landscapes, limiting our ability to improve food supply for pollinators.
We quantify the floral longevity and pollen production of a whole plant community for the first time, enabling us to calculate daily pollen availability. We combine these data with floral abundance and nectar measures from UK farmland to quantify pollen and nectar production at the landscape scale throughout the year.
Pollen and nectar production were significantly correlated at the floral unit, and landscape level. The species providing the highest quantity of pollen on farmland were Salix spp. (38%), Filipendula ulmaria (14%), Rubus fruticosus (10%) and Taraxacum officinale (9%). Hedgerows were the most pollen‐rich habitats, but permanent pasture provided the majority of pollen at the landscape scale, because of its large area. Pollen and nectar were closely associated in their phenology, with both peaking in late April, before declining steeply in June and remaining low throughout the year.
Our data provide a starting point for including pollen in floral resource assessments and ensuring the nutritional requirements of pollinators are met in farmland landscapes.
Knockdown of circXPO1 inhibits the development of oral squamous cell carcinoma cells
Authors: Lu Yao , Bian Jiaqi , Ferrolino Darwin Omaña , Movahed Fatemeh 
Published: April 2024

AbstractBackgroundCircular RNAs (circRNAs) have emerged as pivotal regulators of cellular processes in human malignancies, including oral squamous cell carcinoma (OSCC).MethodsReverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) was used to detect RNA expression levels of circXPO1, miR‐524‐5p and cyclin D1 (CCND1). Colony formation assay and 5‐Ethynyl‐2′‐deoxyuridine (EdU) assay were performed to analyze cell proliferation, while transwell assay was carried out to investigate the cell migration and invasion. Cell apoptosis was assessed by flow cytometry. Protein expression analysis was implemented by Western blot assay. Additionally, lactate production and glucose consumption were investigated using a lactate assay kit and glucose assay kit, respectively. The in vivo tumorigenic potential of circXPO1 was evaluated using a xenograft mouse model assay.ResultsElevated levels of circXPO1 and CCND1, alongside reduced miR‐524‐5p expression were decreased in OSCC tissues and cells. Knockdown of circXPO1 in OSCC cells inhibited their proliferative, migratory and invasive capacities, as well as glycolysis, prompting apoptosis. Moreover, circXPO1 silencing hindered tumor growth in vivo. MiR‐524‐5p could be sequestered by circXPO1, and its inhibition could counteract the beneficial effects of circXPO1 knockdown on OSCC progression.ConclusionKnockdown of circXPO1 inhibited OSCC progression by up‐regulating miR‐524‐5p and down‐regulating CCND1 expression, which might provide potential targets for OSCC treatment.
Clinical characteristics and salivary biomarkers of burning mouth syndrome
Authors: Kong Mi‐Sun , Kim Moon‐Jong , Kim Yoon‐Young , Chang Ji‐Youn , Kho Hong‐Seop 
Published: April 2024

AbstractObjectivesTo investigate the clinical characteristics and salivary biomarkers in each type of burning mouth syndrome (BMS) patients.Materials and MethodsNinety‐eight postmenopausal female patients with BMS were included. Fifty and 21 patients were assigned to the primary and secondary groups, respectively. Twenty‐seven patients with both primary and secondary characteristics were assigned to the intermediate group. Comprehensive clinical characteristics and salivary biomarkers were analyzed.ResultsSignificant differences in age, proportion of hyposalivator patients based on unstimulated whole saliva (UWS), symptom distribution, severties of burning sensation and effect of oral complaints in daily life (Eff‐life), and positive symptom distress index (PSDI) were observed among the three groups. The primary group had significant higher UWS flow rate, fewer UWS hyposalivator proportions, and lesser severity of Eff‐life than the secondary group. The intermediate group had significantly greater intensities of burning sensation and Eff‐life and higher PSDI score than did the primary group. The primary group had significantly higher cortisol and dehydroepiandrosterone (DHEA) levels in stimulated whole saliva than did the secondary group.ConclusionsThis study's findings show that clinical characteristics differentiate each BMS type. Cortisol and DHEA levels are potential salivary biomarkers for discriminating between the primary and secondary types of BMS.
Longitudinal dyadic interplay between marital conflict and psychological well‐being in couples: The moderating roles of wives' employment
Authors: Yu Jeong Jin 
Published: April 2024

AbstractThis study examined longitudinal dyadic and within‐partner associations among self‐esteem, subjective happiness, and marital conflict in married couples, with a possible moderating role of wives' employment status. Data were analyzed from the Panel Study on Korean Children, nationwide longitudinal data. The study sample included 1668 married couples (N = 3336 participants) where both partners provided separate data annually across three waves. Husbands' and wives' mean ages at T1 were 39.3 and 36.8 years, respectively. For both partners, self‐esteem and subjective happiness were related bidirectionally at an individual level. Wives' marital conflict was linked to husbands' subsequent marital conflict and vice versa. Wives played a greater role in their husbands’ self‐esteem than vice versa. Findings suggest that wives tend to be their husband's substantial source of perceived psychological well‐being than the reverse; however, the benefit of psychological well‐being is likely to be lower for husbands of stay‐at‐home wives.
A rare complication of pediatric liver transplantation: Post‐transplant diaphragmatic hernia
Authors: Celtik Ulgen , Dokumcu Zafer , Ozcan Coskun , Ergün Orkan 
Published: April 2024

AbstractAimAcquired post‐transplant diaphragmatic hernia (PTDH) is a rare complication of liver transplantation (LT) in children. We aimed to present our experience in PTDH, and a possible causative background is discussed.MethodsMedical records of patients who had undergone diaphragmatic repair following LT between 2015 and 2023 were reviewed. Demographic information, details of primary diseases necessitating LT, transplantation techniques, and clinical findings associated with PTDH were evaluated.ResultsThere were seven patients with PTDH. Median age at transplantation was 69 (range: 9–200) months. Five patients received a left lateral sector, one patient had a right lobe, and one had a left lobe graft. Time between LT and PTDH was 9 (2–123) months. One patient who was diagnosed in the postoperative 10th year was asymptomatic. Respiratory distress and abdominal pain were the main symptoms among all. All patients underwent laparotomy, and primary repair was performed in six patients, and one patient required mesh repair because of a large defect. Small intestine herniated in most cases. There were two complicated cases with perforation of the stomach and colonic volvulus. There is no recurrence or long‐term complications for the median 60 (20–119) month follow‐up period.ConclusionPTDH is a rare but serious complication. Majority of symptomatic cases present within the first postoperative year, whereas some late‐presenting cases may not be symptomatic. Inadvertent injury to the inferior phrenic vasculatures due to excessive use of cauterization for control of hemostasis may be a plausible explanation in those cases.
Educational needs of community visiting nurses for infection prevention and control: Application of the Borich needs assessment and the Locus for Focus models
Authors: Kim HyungSeon , Choi Dongwon , Shim Haeun , Sohng Kyeong‐Yae , Choi Min‐Jung 
Published: April 2024

AbstractObjectivesThis study aimed to assess the educational needs and analyze the priorities of infection prevention and control (IPC) for community‐visiting nurses.DesignThis is a cross‐sectional descriptive study.SampleThis study was conducted with 144 visiting nurses working in public health centers and long‐term care facilities in South Korea.MethodA total of 23 questions in five subcategories were used to measure the current knowledge and perceived importance of IPC in community‐visiting nursing. Data were collected from June 23 to October 30, 2021, during the COVID‐19 pandemic. Data were analyzed paired t‐test, the Borich needs assessment, and the Locus for Focus models.ResultsTop‐priority content was defined as content belonging to two models, the first 10 contents of Borich needs assessment and the contents located in the Quadrant I of the Locus for Focus models. “Reporting in case of infection‐related accidents,” “Mandatory vaccination for visiting nurses,” “Standard precaution,” “Airborne precaution,” “Contact precautions,” “Respiratory infection control,” and “Post‐visit management.”ConclusionsThis study suggests that it is necessary to provide visiting nurses with more opportunities for IPC education and to develop standardized IPC programs that consider educational priorities.
The effects of photobiomodulation on the improvement of sperm parameters: A review study
Authors: Parvin Ali , Erabi Gisou , Saboohi Tasooji Mohammad Reza , Sadeghpour Sonia , Mellatyar Hassan , Rezaei Arablouydareh Sahar , Navapour Leila , Taheri‐Anganeh Mortaza , Ghasemnejad‐Berenji Hojat 
Published: April 2024

AbstractThe prevalence of male infertility has become a significant clinical concern worldwide, with a noticeable upward trend in recent times. The rates of fertilization and subsequent development of embryos are dependent on many parameters associated with the quality and viability of sperm. Photobiomodulation (PBM) is a promising approach with a great potential for translational applications in the treatment of spermatozoa exhibiting low quality and motility. In this study, a comprehensive analysis of the existing literature, specifically examining the mechanisms of action of PBM has been presented. Our objective was to enhance knowledge in the field of laser light therapy in order to promote the usage of irradiation in clinical settings in a more effective way. Within the realm of reproductive science, the utilization of PBM has been employed to enhance the metabolic processes, motility, and viability of spermatozoa. This is attributed to its advantageous effects on mitochondria, resulting in the activation of the mitochondrial respiratory chain and subsequent synthesis of ATP. This therapeutic approach can be highly advantageous in circumventing the reliance on chemical substances within the culture medium for spermatozoa while also facilitating the viability and motility of spermatozoa, particularly in circumstances involving thawing or samples with significant immotility.
Novel D‐π‐A hemicyanine dye as photoinitiators for in situ hydrogel formation and DLP printing
Authors: Du Yao , Zhang Yating , Liu Shitao , Zhang Xiwang , Wang Tao 
Published: April 2024

AbstractThe field of biofabrication imposes stringent requirements on the polymerization activity and biosafety of photopolymeric hydrogel systems. In this investigation, we designed and synthesized four hemicyanine dyes with a D‐π‐A structure specifically tailored for biofabrication purposes. These novel dyes, incorporating carbazole (CZ), triphenylamine (TPA), anthracene (AN), and benzodithiophene (BDT) as electron donors, along with heterocyclic salt (IN) as electron acceptors, were prepared using a straightforward synthesis method. The absorption maxima of ANIN, CZIN, and TPAIN exceeded 500 nm, rendering them suitable co‐initiators for the free radical photopolymerization of acrylates under green‐red light exposure facilitated by light‐emitting diodes (LEDs) and the co‐initiator iodonium salt (ION). Notably, CZIN and TPAIN, due to their robust dye absorption and efficient electron transfer to ION, functioned as high‐performance photosensitizers. Meanwhile, BDTIN, with its strong and broad absorption range (400–600 nm), enhanced the accuracy of visible light photopolymerization. These dyes exhibit characteristics such as facile synthesis, heightened photo stability, and non‐toxicity and also demonstrate the ability to discern the alkalinity of a solution to some extent. Furthermore, we explored the application of these hemicyanine dyes in 3D printing, showing potential to enhance printing resolution in DLP 3D printing (digital light process 3D printing).
Advocacy coalitions or pragmatic coupling of streams? Explaining policy change in Mexico: The tax reforms of Vicente Fox and Enrique Peña (2001 and 2013)
Authors: Mena Aguilar Oswaldo A. 
Published: April 2024

AbstractWhen the former hegemonic PRI won the 2012 election after 12 years of rule by PAN, the President shared with his predecessors a similar institutional setting and divided government. And yet, the PRI managed to pass all items of an extensive 2013 tax reform. If the setting was the same, why did PRI succeed when PAN had only limited success? Why was the 2013 tax reform progressive if PAN and PRI were ideologically proximate? Current explanations that characterize PAN's 2001 tax reforms as a failure because of an unsuccessful change to the Value‐Added Tax Law can accommodate neither the puzzling 2013 extensive tax overhaul nor the changes to the Income Tax Law that were indeed approved in 2001. To navigate these puzzles, I contrast the elements of both proposals and rely on two public policy theoretical frameworks literature, the Advocacy Coalitions and Multiple Streams frameworks to find that, despite representing political compromise over a shared agenda, the outcomes in 2001 and 2013 had less to do with commitment to a core of mutual beliefs and more with policy entrepreneurs of PRI and PRD taking a gamble on a window of opportunity.
Repeated colonization of the Northern Limestone Alps from the Southern Limestone Alps by the rock‐dwelling snail species Cochlostoma henricae
Authors: Hausdorf Bernhard , Xu Jie , Bamberger Sonja 
Published: April 2024

AbstractHausdorf, B., Xu, J., BAMBERGER, S. (2024) Repeated colonization of the Northern Limestone Alps from the Southern Limestone Alps by the rock‐dwelling snail species Cochlostoma henricae—Zoologica Scripta, 00, 000–000. The evolution of biogeographic disjunctions in the Southern and Northern Limestone Alps has been studied only in a few taxa. Cochlostoma henricae (Strobel, 1851) is a land snail species restricted to limestone rocks showing such disjunctions. We studied the phylogeny and population structure of this species using genomic ddRAD data to better understand the differentiation of the species in the Southern Alps and the origin of the isolated occurrences in the Northern Limestone Alps. Phylogenetic and population‐genetic analyses of the ddRAD data revealed a subdivision of the C. henricae populations into a western cluster and an eastern cluster. These clusters were probably separated by glaciers that extended from the Piave Valley to the margin of the Alps. Such a longitudinal subdivision is a common pattern of several species in the Southern Limestone Alps. However, the boundaries between western and eastern population groups differ between taxa. Cochlostoma henricae colonized the Northern Limestone Alps at least twice. The population from Breitenwang in Tyrol originated from the western group, whereas the occurrences in the Salzkammergut are from the eastern group. These and similar disjunctions of several other land snails show that even species with limited dispersal abilities could cross the Alps repeatedly by passive long‐distance dispersal events. A subdivision of C. henricae into three subspecies, proposed based on shell characters, does not correspond to its population‐genetic structure. Instead, the eastern and western population groups represent distinct evolutionary units.
Multilocus genetic and morphological phylogenetic analysis: Unveiling a new genus and species in the Tribe Nannenini of jumping spiders (Araneae, Salticidae)
Authors: Satkunanathan Abira , Benjamin Suresh P. 
Published: April 2024

AbstractJumping spiders (Salticidae) are a diverse group of non‐web‐building predators and the most species‐rich spider family. The Salticidae Tribe Nannenini consists of a small group of Oriental jumping spiders that are very poorly known. The use of explicit phylogenetic analyses to establish its affinities has so far remained limited. The Nannenini genus Epidelaxia has a peculiar morphology, making its systematic position uncertain. In this study, we present a comprehensive dataset combining molecular and morphological data for Nannenini, including exemplars from all genera. This dataset comprises DNA sequences of approximately 2400 bp, including nuclear genes (18S, 28S and H3) and the mitochondrial gene CO1, sequenced for 40 taxa (20 ingroups and 20 outgroups), along with 61 morphological characters. Notably, both DNA sequences and morphological traits were analysed separately and concurrently for the first time. The Nannenini clade is recovered with high support. Further, Epidelaxia is monophyletic and sister to Tubalaxia gen. n. This is the first hypothesis on the internal phylogenetic structure of Epidelaxia and its placement within the Tribe Nannenini. A new genus Tubalaxia gen. n. and the following new species are described: E. bharathi sp. n., E. somasundaram sp. n., T. castanea sp. n., T. aurea sp. n. The following new combination is proposed: Tubalaxia minuta (Prószyński, 1992) comb. n. Epidelaxia albostellata, E. albocruciata and E. obscura are redescribed. It is worth noting that the newly described species face endangerment due to their limited distribution and small population sizes.
Optical recordings of organellar membrane potentials and the components of membrane conductance in lysosomes
Authors: Castillo‐Velasquez Cristian , Matamala Ella , Becerra Diego , Orio Patricio , Brauchi Sebastian E. 
Published: April 2024

AbstractThe eukaryotic cell is highly compartmentalized with organelles. Owing to their function in transporting metabolites, metabolic intermediates and byproducts of metabolic activity, organelles are important players in the orchestration of cellular function. Recent advances in optical methods for interrogating the different aspects of organellar activity promise to revolutionize our ability to dissect cellular processes with unprecedented detail. The transport activity of organelles is usually coupled to the transport of charged species; therefore, it is not only associated with the metabolic landscape but also entangled with membrane potentials. In this context, the targeted expression of fluorescent probes for interrogating organellar membrane potential (Ψorg) emerges as a powerful approach, offering less‐invasive conditions and technical simplicity to interrogate cellular signalling and metabolism. Different research groups have made remarkable progress in adapting a variety of optical methods for measuring and monitoring Ψorg. These approaches include using potentiometric dyes, genetically encoded voltage indicators, hybrid fluorescence resonance energy transfer sensors and photoinduced electron transfer systems. These studies have provided consistent values for the resting potential of single‐membrane organelles, such as lysosomes, the Golgi and the endoplasmic reticulum. We can foresee the use of dynamic measurements of Ψorg to study fundamental problems in organellar physiology that are linked to serious cellular disorders. Here, we present an overview of the available techniques, a survey of the resting membrane potential of internal membranes and, finally, an open‐source mathematical model useful to interpret and interrogate membrane‐bound structures of small volume by using the lysosome as an example.
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