bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood-based biological ageing and red cell distribution width are associated with prevalent Parkinson’s disease: findings from a large Italian population cohort
Authors: Gialluisi Alessandro , Tirozzi Alfonsina , Costanzo Simona , De Bartolo Maria Ilenia , Belvisi Daniele , Magnacca Sara , De Curtis Amalia , Falciglia Stefania , Ricci Moreno , Cerletti Chiara , Donati Maria Benedetta , Berardelli Alfredo , de Gaetano Giovanni , Iacoviello Licia 
Published: April 2024

BackgroundAging clocks tag the actual underlying age of an organism and its discrepancy with chronological age and have been reported to predict incident disease risk in the general population. However, the relationship with neurodegenerative risk and in particular with Parkinson’s Disease (PD) remains unclear, with few discordant findings reporting associations with both incident and prevalent PD risk.ObjectiveTo clarify this relationship, we computed a common aging clock based on blood markers and tested the resulting discrepancy with chronological age (ΔPhenoAge) for association with both incident and prevalent PD risk.MethodsIn a large Italian population cohort - the Moli-sani study (N=23,437; age ≥ 35 years; 52% women) - we carried out both Cox Proportional Hazards regressions modelling ΔPhenoAge as exposure and incident PD as outcome, and linear models testing prevalent PD as exposure and ΔPhenoAge as outcome. All models were incrementally adjusted for age, sex, education level completed and other risk/protective factors previously associated with PD risk in the same cohort (prevalent dysthyroidism, hypertension, diabetes, use of oral contraceptives, exposure to paints, daily coffee intake and cigarette smoking).ResultsNo significant association between incident PD risk (209 cases, median (IQR) follow-up time 11.19 (2.03) years) and PhenoAging was observed (Hazard Ratio [95% Confidence Interval] = 0.98 [0.71; 1.37]). However, a small but significant increase of ΔPhenoAge was observed in prevalent PD cases vs healthy subjects (β (Standard Error) = 1.39 (0.70)). An analysis of each component biomarker of PhenoAge revealed a significant positive association of prevalent PD status with red cell distribution width (RDW; β (SE) = 0.46 (0.18)). All the remaining markers did not show any significant evidence of association.ConclusionThe reported evidence highlights systemic effects of prevalent PD status on biological aging and red cell distribution width. Further cohort and functional studies may help shedding a light on the related pathways altered at the organism level in prevalent PD, like red cells variability, inflammatory and oxidative stress mechanisms.
A new method for predicting the oil displacement volume by waterflood huff-puff of the fractured-cavity type carbonate oil reservoirs
Authors: Peng Cao , Peng Wang , Zhiliang Liu , Jiajun Chen , Guohui Li , Yifan Du 
Published: April 2024

Introduction: More than two billion tons of oil reserves have been discovered in the fractured-cavity type carbonate reservoirs in Tarim Basin, which is a key field for increasing reserves and production in the future. This type of oil reservoirs is characterized by extremely high heterogeneity, and waterflood huff-puff oil displacement is generally used for secondary development of the oil reservoirs. The conventional methods are dominated by qualitative evaluation, which are insufficient to predict the oil production increase by waterflood huff-puff in various karst zones.Methods: The new method developed in this study involves four aspects, i.e., establishing a standard well database, optimally selecting the key development indicators, equivalent classification of reservoir space type, and optimally selecting the classification and evaluation methods for oil production increase by waterflood huff-puff. From the perspective of reservoir space type, the result of waterflood huff-puff oil displacement of multi-cave type reservoir (large scale) is better than that of relatively isolated cave type reservoir, followed by the fractured-vuggy type reservoir.Results and discussion: The new method shows advantages in the following aspects: 1) The differences in reservoir geological genesis and spatial structure characteristics in various karst zones are involved; 2) The difference in internal filling features of the same type of reservoir is integrally determined based on the changes in production performance; 3) The actual development characteristics of waterflood huff-puff oil displacement wells in various karst zones are systematically analyzed, and the criteria for selecting standard wells is formulated; 4) The violin plot is introduced as the main study tool for predicting and discriminating oil production increase by waterflood huff-puff displacement. Therefore, the new method is more capable of predicting the oil production increase by waterflood huff-puff in various karst zones.
Comparison of the effectiveness four years after Homo/Hetero prime-boost with 10 μg HP and 20 μg CHO recombinant hepatitis B vaccine at 1 and 6 months in maternal HBsAg-negative children
Authors: Yin Zhiying , Wen Tingcui , Fu Canya , Li Junji , Fang Quanjun , Gong Xiaoying , You Jialing , Wang Shuangqing , Zheng Canjie 
Published: April 2024

IntroductionLimited data were available on the effectivenessfour years after Homo or Hetero prime-boost with 10 μg Hansenulapolymorpha recombinant hepatitis B vaccine (HepB-HP) and 20 μgChinese hamster ovary cell HepB (HepB-CHO).MethodsA crosssectional study was performed in maternalhepatitis B surface antigen (HBsAg)-negative children whoreceived one dose of 10 μg HepB-HP at birth, Homo or Heteroprime-boost with 10 μg HepB-HP and 20 μg HepB-CHO at 1 and 6months. HBsAg and hepatitis B surface antibody (anti-HBs) fouryears after immunization were quantitatively detected by achemiluminescent microparticle immunoassay (CMIA).ResultsA total of 359 children were included; 119 childrenreceived two doses of 10 μg HepB-HP and 120 children receivedtwo doses of 20 μg HepB-CHO, called Homo prime-boost; 120children received Hetero prime-boost with 10 μg HepB-HP and 20μg HepB-CHO. All children were HBsAg negative. The geometricmean concentration (GMC) and overall seropositivity rate (SPR) ofanti-HBs were 59.47 (95%CI: 49.00 – 72.16) mIU/ml and 85.51%(307/359). Nearly 15% of the study subjects had an anti-HBsconcentration &lt; 10 mIU/ml and 5.01% had an anti-HBsconcentration ≤ 2.5 mIU/ml. The GMC of the 20 μg CHO Homoprime-boost group [76.05 (95%CI: 54.97 – 105.19) mIU/ml] washigher than that of the 10 μg HP Homo group [45.86 (95%CI:31.94 – 65.84) mIU/ml] (p = 0.035). The GMCs of the Heteroprime-boost groups (10 μg HP-20 μg CHO and 20 μg CHO-10 μgHP) were 75.86 (95% CI: 48.98 – 107.15) mIU/ml and 43.65(95%CI: 27.54 – 69.18) mIU/ml, respectively (p = 0.041). Aftercontrolling for sex influence, the SPR of the 20 μg CHO Homoprime-boost group was 2.087 times than that of the 10 μg HPHomo group.DiscussionThe HepB booster was not necessary in the generalchildren, Homo/Hetero prime-boost with 20 μg HepB-CHO wouldincrease the anti-HBs concentration four years after immunization,timely testing and improved knowledge about the self-pay vaccinewould be good for controlling hepatitis B.
Bioethical knowledge in students and health professionals: a systematic review
Authors: González-Blázquez Francisco Javier , Ruiz-Hontangas Antonio , López-Mora Clara 
Published: April 2024

IntroductionBioethics training is essential for healthcare professionals as it enables them to address ethical dilemmas in their clinical practice. However, there is still a lack of rigorous teaching programs, and assessing bioethical knowledge poses challenges.Methodology:Systematic review using the PRISMA method.ResultsAnalysis of 27 studies reveals a lack of ethical knowledge and skills among healthcare professionals and students. Specific training in bioethics is effective in developing bioethical competencies. Different approaches have been employed, including integrated training in academic curricula and intensive or ongoing programs. The results demonstrate improvements in knowledge, attitudes, and ethical values, although regularly updating these courses is recommended.ConclusionSpecific training, institutional support, and considering regional and disciplinary differences are necessary to enhance ethics in the practice of healthcare professionals.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023437146, identifier CRD42023437146
Knowledge, attitude, and practice of psoriasis patients toward their diseases: a web-based, cross-sectional study
Authors: Tian Jun , Zhang Lei , Zhao Xiangrong , Yang Li 
Published: April 2024

ObjectiveTo investigate the knowledge, attitude, and practice (KAP) of psoriasis patients toward the disease.MethodsA web-based cross-sectional study was conducted among psoriasis patients who were diagnosed at the outpatient of Shaanxi Provincial People’s Hospital in March 2023. A self-designed questionnaire was administered for data collection and KAP assessment.ResultsA total of 526 valid questionnaires were included, including 257 males (48.86%) psoriasis patients. Their mean KAP scores were 8.09 ± 3.60 (possible range: 0–12), 31.94 ± 4.61 (possible range: 10–50), and 51.92 ± 8.83 (possible range: 15–75), respectively. Pearson’s correlation analysis showed a positive correlation between knowledge and attitude (r = 0.186, p &lt; 0.001), a positive correlation between knowledge and practice (r = 0.313, p &lt; 0.001), and a negative correlation between attitude and practice (r = −0.181, p &lt; 0.001). Moreover, structural equation model showed that medication (β = 2.74, 95% CI: 2.17, 3.32, p &lt; 0.001) has significantly positive effect on knowledge. Education (β = 0.56, 95% CI: 0.31, 0.81, p &lt; 0.001) and duration of psoriasis (β = 1.01, 95% CI: 0.54, 1.49, p &lt; 0.001) have significantly positive effect on attitude. Knowledge (β = 1.03, 95% CI: 0.80, 1.26, p &lt; 0.001) and medication (β = 4.59, 95% CI: 2.78, 6.40, p &lt; 0.001) has significantly positive effect on practice, while attitude (β = −0.41, 95% CI: −0.57, −0.26, p &lt; 0.001) and duration of psoriasis (β = −2.53, 95% CI: −3.49, −1.57, p &lt; 0.001) exhibit significantly negative effect on practice.ConclusionPsoriasis patients have good knowledge, positive attitude, and proactive practice toward the disease. Education, medication, duration of psoriasis might have effect on their KAP.
The palliative care needs and experiences of patients with advanced Parkinson’s disease: a qualitative scoping review
Authors: Lou Yan , Li Yiting , Chen Yiping 
Published: April 2024

AimTo determine the experiences and needs of palliative care in patients with advanced Parkinson’s disease (PD).MethodsA scoping literature review methodology, as described by the Joanna Briggs Institute, was employed to search for relevant literature. An electronic search of studies published in English was conducted across five databases from inception to 10 September 2023.ResultsThe search yielded a total of 1,205 articles, with 20 meeting the inclusion criteria. The findings were organized into four themes: (1) unmet emotional and informational needs; (2) needs for effective coordination of care; (3) planning for the future; and (4) symptom management. This scoping review highlights the intricate nature of palliative care for patients with PD and sheds light on issues within current palliative care healthcare systems. The findings emphasize the necessity for individualized interventions and services to address the diverse unmet palliative care needs of people with PD.ConclusionThe study reveals the complex landscape of palliative care for individuals with advanced PD, emphasizing the inadequacies within existing healthcare systems. The identified themes underscore the importance of tailored interventions to address the varied unmet palliative care needs of this population.
Impact of suppression of the SOS response on protein expression in clinical isolates of Escherichia coli under antimicrobial pressure of ciprofloxacin
Authors: Recacha Esther , Kuropka Benno , Díaz-Díaz Sara , García-Montaner Andrea , González-Tortuero Enrique , Docobo-Pérez Fernando , Rodríguez-Rojas Alexandro , Rodríguez-Martínez Jose Manuel 
Published: April 2024

Introduction/objectiveSuppression of the SOS response in combination with drugs damaging DNA has been proposed as a potential target to tackle antimicrobial resistance. The SOS response is the pathway used to repair bacterial DNA damage induced by antimicrobials such as quinolones. The extent of lexA-regulated protein expression and other associated systems under pressure of agents that damage bacterial DNA in clinical isolates remains unclear. The aim of this study was to assess the impact of this strategy consisting on suppression of the SOS response in combination with quinolones on the proteome profile of Escherichia coli clinical strains.Materials and methodsFive clinical isolates of E. coli carrying different chromosomally- and/or plasmid-mediated quinolone resistance mechanisms with different phenotypes were selected, with E. coli ATCC 25922 as control strain. In addition, from each clinical isolate and control, a second strain was created, in which the SOS response was suppressed by deletion of the recA gene. Bacterial inocula from all 12 strains were then exposed to 1xMIC ciprofloxacin treatment (relative to the wild-type phenotype for each isogenic pair) for 1 h. Cell pellets were collected, and proteins were digested into peptides using trypsin. Protein identification and label-free quantification were done by liquid chromatography-mass spectrometry (LC–MS) in order to identify proteins that were differentially expressed upon deletion of recA in each strain. Data analysis and statistical analysis were performed using the MaxQuant and Perseus software.ResultsThe proteins with the lowest expression levels were: RecA (as control), AphA, CysP, DinG, DinI, GarL, PriS, PsuG, PsuK, RpsQ, UgpB and YebG; those with the highest expression levels were: Hpf, IbpB, TufB and RpmH. Most of these expression alterations were strain-dependent and involved DNA repair processes and nucleotide, protein and carbohydrate metabolism, and transport. In isolates with suppressed SOS response, the number of underexpressed proteins was higher than overexpressed proteins.ConclusionHigh genomic and proteomic variability was observed among clinical isolates and was not associated with a specific resistant phenotype. This study provides an interesting approach to identify new potential targets to combat antimicrobial resistance.
Safety and feasibility of optimized transcranial direct current stimulation in patients with mild cognitive impairment due to Alzheimer’s disease: a multicenter study protocol for a randomized controlled trial
Authors: Kim TaeYeong , Kang Dong Woo , Salazar Fajardo Jhosedyn Carolaym , Jang Hanna , Um Yoo Hyun , Kim Sunghwan , Wang Sheng-Min , Kim Donghyeon , Lim Hyun Kook 
Published: April 2024

IntroductionTranscranial direct current stimulation (tDCS) may effectively preserve and improve cognitive function in patients with mild cognitive impairment (MCI). Research has shown that Individual brain characteristics can influence the effects of tDCS. Computer three-dimensional brain modeling based on magnetic resonance imaging (MRI) has been suggested as an alternative for determining the most accurate tDCS electrode position based on the patients’ individual brain characteristics to enhance tDCS effects. Therefore, this study aims to determine the feasibility and safety of applying tDCS treatment using optimized and personalized tDCS electrode positions in patients with Alzheimer’s disease (AD)-induced MCI using computer modeling and compare the results with those of a sham group to improve cognitive function.MethodA prospective active-sham group feasibility study was set to recruit 40 participants, who will be randomized into Optimized-tDCS and Sham-tDCS groups. The parameters for tDCS will be 2 mA (disk electrodes R = 1.5 cm) for 30 min during two sets of 15 sessions (2 weeks of resting period in between), using two electrodes in pairs. Using computer modeling, the tDCS electrode positions of each participant will be personalized. Outcome measurements are going to be obtained at three points: baseline, first post-test, and second post-test. The AD assessment scale-cognitive subscale (ADAS-Cog) and the Korean version of Mini-Mental State Examination (K-MMSE), together with other secondary outcomes and safety tests will be used.DiscussionFor the present study, we hypothesize that compared to a sham group, the optimized personalized tDCS application would be effective in improving the cognitive function of patients with AD-induced MCI and the participants would tolerate the tDCS intervention without any significant adverse effects.Clinical trial registration: https://cris.nih.go.kr, identifier [KCT0008918].
Prognostic significance vestibular examination results in patients with vestibular migraine
Authors: Goto Fumiyuki , Wasano Koichiro , Kaneda Shoji , Okami Kenji 
Published: April 2024

IntroductionVestibular migraine (VM) is a newly defined clinical condition. Several vestibular abnormalities have been reported in patients with VM. However, to date, no specific vestibular examinations are used to define VM. Therefore, the utility of vestibular examinations is limited. Currently, the role of vestibular examination has not been clearly defined. We speculated that the results of vestibular examinations could predict the prognosis of VM. We investigated the relationship between the vestibular examination results and clinical outcomes in patients with VM.MethodsThis study included 25 patients with VM. Vestibular examinations, including the video head impulse test (V-HIT), cervical and ocular vestibular evoked myogenic potential (c-VEMP and o-VEMP), posturography, and several questionnaires, including the Dizziness Handicap Inventory (DHI), were conducted at the initial evaluation. Lifestyle modifications for VM and conventional pharmacological prophylactic treatments, including lomerizine, amitriptyline, and valproic acid, were performed. After 4 weeks of treatment, clinical improvements were evaluated using the Clinical Global Improvement Scale (CGI-s). The relationships among the CGI-S score, several clinical variables, and the results of several vestibular examinations were evaluated. Each patient was further classified into two subgroups according to treatment outcomes concerning vertigo and headache: CGI-S score from 0 to 2 (good response [GR]) and CGI-S score &gt; 3 (poor response [PR]).ResultsOverall, after treatment, most of the patients had improved dizziness and headache, and the CGI-s was 2.7 ± 1.3. There were 12 GRs, and 13 had PRs. Thus, neither V-HIT nor posturography predicted the prognosis. For c-VEMP, patients with GRs had significantly small AR concerning PR (19.2 ± 12.8 and 62.5 ± 42.5, respectively, [p &lt; 0.01]). There were five normal, six unilateral, and 14 bilateral no response in 500hz o-VEMP. CGI-s of normal, unilateral, and bilateral no response was 1.4 ± 0.5, 2.8 ± 1.3, and 3.1 ± 1.2, respectively. There was a statistically significant difference between the normal and bilateral non-response o-VEMP groups (p &lt; 0.05).ConclusionPatients with VM had improvements in both headache and vertigo through a combination of lifestyle changes and prophylactic medications. Vestibular examinations, especially o- or c-VEMP, are beneficial for predicting the treatment outcomes of VM. The pathophysiology of VM is closely related to vestibular abnormalities, particularly the otolith-related pathways.
A pilot randomized controlled trial of transcranial direct current stimulation adjunct to moderate-intensity aerobic exercise in hypertensive individuals
Authors: Silva-Filho Edson , Bikson Marom , Gebodh Nigel , Khadka Niranjan , da Cruz Santos Amilton , Pegado Rodrigo , do Socorro Brasileiro-Santos Maria 
Published: April 2024

BackgroundHypertension is a global issue that is projected to worsen with increasingly obese populations. The central nervous system including the parts of the cortex plays a key role in hemodynamic stability and homeostatic control of blood pressure (BP), making them critical components in understanding and investigating the neural control of BP. This study investigated the effects of anodal transcranial direct current stimulation (tDCS) associated with aerobic physical exercise on BP and heart rate variability in hypertensive patients.MethodsTwenty hypertensive patients were randomized into two groups: active tDCS associated with aerobic exercise or sham tDCS associated with aerobic exercise. BP and heart rate variability were analyzed before (baseline) and after twelve non-consecutive sessions. After each tDCS session (2 mA for 20 min), moderate-intensity aerobic exercise was carried out on a treadmill for 40 min.ResultsA total of 20 patients were enrolled (53.9 ± 10.6 years, 30.1 ± 3.7 Kg/m2). There were no significant interactions between time and groups on diastolic BP during wake, sleep, over 24 and 3 h after the last intervention. Heart rate variability variables showed no significant difference for time, groups and interaction analysis, except for HF (ms2) between groups (p &lt; 0.05).ConclusionAnodal tDCS over the temporal cortex associated with aerobic exercise did not induce improvements in BP and heart rate variability.Clinical trial registrationhttps://ensaiosclinicos.gov.br/rg/RBR-56jg3n/1, identifier: RBR-56jg3n.
A study demonstrating users’ preference for the adapted-REQUITE patient-reported outcome questionnaire over PRO-CTCAE® in patients with lung cancer
Authors: Jordan Thomas , Nuamek Thitikorn , Fornacon-Wood Isabella , Califano Raffaele , Coote Joanna , Harris Margaret , Mistry Hitesh , Taylor Paul , Woolf David , Faivre-Finn Corinne 
Published: April 2024

IntroductionThe use of patient-reported outcomes (PROs) has been shown to enhance the accuracy of symptom collection and improve overall survival and quality of life. This is the first study comparing concordance and patient preference for two PRO tools: Patient-Reported Outcomes version of the Common Terminology Criteria for Adverse Events (PRO-CTCAE®) and the adapted-REQUITE Lung Questionnaire.Materials and MethodsPatients with lung cancer were recruited to the study while attending outpatient clinics at a tertiary cancer centre. Clinician-reported outcomes were generated through initial patient assessment with CTCAE v4.03. Participants then completed the PRO-CTCAE® and adapted-REQUITE questionnaires. Concordance between the 2 questionnaires was assessed by calculating Pearson correlation coefficient. PRO-CTCAE® and CTCAE concordance was demonstrated by calculating Pearson correlation coefficient from the linear predictors of an ordinal logistic regression. P-values were also calculated.ResultsOut of 74 patients approached, 65 provided written informed consent to participate in the study. 63 (96.9%) patients completed both PRO-CTCAE® and adapted-REQUITE questionnaires. Pearson correlation coefficient between PRO tools was 0.8-0.83 (p &lt;.001). Correlation between CTCAE and PRO-CTCAE® ranged between 0.66-0.82 (p &lt;.001). Adapted-REQUITE and CTCAE correlation was higher for all symptoms ranging between 0.79-0.91 (p &lt;.001). Acceptable discrepancies within one grade were present in 96.8%-100% of symptom domains for REQUITE and in 92.1%-96.8% for all domains in the PRO-CTCAE®. 54% of the total participant cohort favored the adapted-REQUITE questionnaire due to reduced subjectivity in the questions and ease of use.ConclusionThe adapted-REQUITE questionnaire has shown a superior correlation to clinician-reported outcomes and higher patient preference than the PRO-CTCAE®. The results of this study suggest the use of the REQUITE questionnaire for patients with lung cancer in routine clinical practice.