bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Mechanisms of Medicinal Plant Securinega suffruticosa-derived
Compound Securinine against Spinal Muscular Atrophy based on Network
Pharmacology and Experimental Verification
Authors: Zhang Yinhong , He Jing , Xiang Lifeng , Tang Xinhua , Wang Shiyu , Li Aoyu , Wang Chaoyan , Li Li , Zhu Baosheng 
Published: April 2024

Background:
Spinal Muscular Atrophy (SMA) is a severe motor neuronal disorder with high morbidity
and mortality. Securinine has shown the potential to treat SMA; however, its anti-SMA role remains unclear.

Objective:
This study aims to reveal the anti-SMA mechanisms of securinine.

Methods:
Securinine-associated targets were acquired from Herbal Ingredients' Targets (HIT), Similarity
Ensemble Approach (SEA), and SuperPred. SMA-associated targets were obtained from GeneCards and Dis-
GeNET. Protein-protein interaction (PPI) network was constructed using GeneMANIA, and hug targets were
screened using cytoHubba. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)
analyses were performed using ClusterProfifiler. Molecular docking was conducted using Pymol and Auto-
Dock. In vitro assays were used to verify the anti-SMA effects of securinine.

Results:
Twenty-six intersection targets of securinine and SMA were obtained. HDAC1, HDAC2, TOP2A,
PIK3R1, PRMT5, JAK2, HSP90AB1, TERT, PTGS2, and PAX8 were the core targets in PPI network. GO
analysis demonstrated that the intersecting targets were implicated in the regulation of proteins, steroid hormones,
histone deacetylases, and DNA transcription. KEGG analysis, pathway-pathway, and hub target-pathway
networks revealed that securinine might treat SMA through TNF, JAK-STAT, Ras, and PI3K-Akt pathways.
Securinine had a favorable binding affinity with HDAC1, HSP90AB, JAK2, PRMT5, PTGS2, and
TERT. Securinine rescued viability suppression, mitochondria damage, and SMN loss in the SMA cell model.
Furthermore, securinine increased HDAC1 and PRMT5 expression, decreased PTGS2 expression, suppressed
the JAK2-STAT3 pathway, and promoted the PI3K-Akt pathway.

Conclusion:
Securinine might alleviate SMA by elevating HDAC1 and PRMT5 expression and reducing PTGS2
via JAK2-STAT3 suppression and PI3K-Akt activation.
Synthesis, Characterization, Antimicrobial Activity, and Molecular Docking Study of
Newer Chalcone-based Triazolo Pyrimidine Compounds
Authors: Gohil Monik , Das Siva Prasad , Boruah Jeena Jyoti 
Published: April 2024

Abstract::
In this work, we present the synthesis of a newer series of 15 chalcone-based
pyrimidine compounds 4a-o. All the compounds were characterized by elemental analysis,
melting point determination, mass, FTIR, and NMR analysis. We have evaluated the antimicrobial
activity of these compounds. The compounds showed good inhibition activity
towards different bacterial and fungal species such as S. aureus, S. pneumonia, E. coli, P.
aeruginosa, Candida albicans, Aspergillus niger, and Alternaria alternata. Compounds 4c,
4h, 4k, and 4g showed comparable activities to those of commercially available drugs. Molecular
docking study showed good interaction between each of the compounds and DNA
gyrase enzyme. The docking score of the compounds ranges between -8.0 to -8.9 kcal/mol.
Further, the ADMET analysis indicated the potential of the compounds as a drug candidate.
Analysis of the Relationship between Recent Small Subcortical Infarcts
and Autonomic Nervous Dysfunction
Authors: Yuan Wenxin , An Lu , Wang Yunchao , Zong Ce , Yang Yinghao , Jin Hua , Gao Yuan , Wang Limei , Li Yusheng , Xu Yuming , Ji Yan 
Published: April 2024

Objective:
Autonomic Nervous System (ANS) dysfunction may be involved in the
pathogenesis of Cerebral Small Vessel Disease (CSVD). The study aimed to explore the relationship
between Recent Small Subcortical Infarct (RSSI) and Blood Pressure Variability (BPV),
and Heart Rate Variability (HRV).

Methods:
A total of 588 patients from the CSVD registration research database of Henan Province
were included in this study, and were divided into two groups according to the presence of
RSSI. Clinical data, including demographic characteristics, disease history, laboratory indexes,
24-hour ambulatory blood pressure and electrocardiogram indicators, and imaging markers of
CSVD, were collected. Univariate and binary logistic regression analyses were used to study the
relationship between RSSI and indicators of laboratory, HRV and BPV in the CSVD population.

Results:
Multivariate analysis showed that higher 24-hour mean Diastolic Blood Pressure
(DBP)[Odds Ratios (OR)=1.083,95% Confidence Intervals (CI)=(1.038,1.129), p < 0.001],
Standard Deviation (SD) of 24-hour DBP [OR=1.059,95%CI=(1.000,1.121), p = 0.049], nocturnal
mean Systolic Blood Pressure (SBP) [OR=1.020,95%CI=(1.004,1.035), p = 0.012], nocturnal
mean DBP [OR=1.025,95%CI=(1.009,1.040), p = 0.002] were independent risk factors for
RSSI. In contrast, the decrease of the standard deviation of N–N intervals (SDNN)
[OR=0.994,95%CI=(0.989,1.000), p = 0.035] was beneficial to the occurrence of RSSI. In addition,
neutrophil counts [OR=1.138,95%CI=(1.030,1.258), p = 0.011], total cholesterol (TC)
[OR=1.203,95%CI=(1.008,1.437), p = 0.041] and High-Density Lipoprotein (HDL) [OR=0.391,
95%CI=(0.195,0.786), p = 0.008] were also independently associated with the occurrence of
RSSI. After adjusting for confounding factors, except for TC, the other factors remained associated
with the occurrence of RSSI.

Conclusion:
Increased 24-hour mean DBP, nocturnal mean SBP and DBP, SD of 24-hour DBP
and decreased SDNN were independently correlated with RSSI occurrence, suggesting that
sympathetic overactivity plays a role in the pathogenesis of RSSI.
Approaches Towards Better Immunosuppressive Agents
Authors: Walczak Juliusz , Iwaszkiewicz-Grześ Dorota , Cholewiński Grzegorz 
Published: April 2024

Abstract:
Several classes of compounds are applied in clinics due to their immunosuppressive properties in transplantology and the treatment of autoimmune diseases. Derivatives of mycophe-nolic acid, corticosteroids and chemotherapeutics bearing heterocyclic moieties like methotrexate, azathioprine, mizoribine, and ruxolitinib are active substances with investigated mechanisms of action. However, improved synthetic approaches of known drugs and novel derivatives are still being reported to attempt better accessibility and therapeutic properties. In this review article, we present the synthesis of the designed chemical structures based on recent literature reports con-cerning novel compounds as promising immunosuppressive drugs. Moreover, some of the dis-cussed derivers revealed also other types of activities with prospective medicinal potential.
Designing a Novel Multi-epitope Peptide as a Potential Serodiagnosis
Marker for the Diagnosis of Acinetobacter baumannii: An In silico
Approach
Authors: Rezaee Maryam , Mohammadi Mohsen , Savardashtaki Amir , Rahbar Mohammad Reza , Nezafat Navid 
Published: April 2024

Background:
Acinetobacter baumannii is an opportunistic pathogen that causes many
infections, including nosocomial infections; this bacterium has a high mortality rate among other
bacteria. A. baumannii has an elastic genome that changes rapidly when exposed to harsh environmental
conditions, leading to widespread bacterial resistance to various disinfectants and antibiotics.
The high ability of bacteria to bind to all surfaces and survive in different conditions has
caused the spread of bacteria in various environments. Rapid detection is very important in preventing
the spread and even treatment of the infection.

Methods:
Currently, the Polymerase Chain Reaction (PCR) method is the only effective method
used for diagnosis, which has some pros and cons.

Results and Conclusion:
This study aimed to design a new recombinant multi-epitope protein
from Acinetobacter baumannii that can be used in ELISA for rapid diagnosis. The unique feature
of this study from others is the use of patient serum for antibody monitoring.
Ethnobotanical Use, Phytochemistry and Pharmacological Activities of Clerodendrum colebrookianium (Lamiaceae)
Authors: Teli Aniya , Jena Diptimayee , Saikia Lunasmrita , Mondal Nur Shaid , Sen Saikat , Mishra Kirtimaya , Gautam Manish Kumar 
Published: April 2024

:
Clerodendrum colebrookianium (CC) is a small shrub belonging to the genus Clerondendrum
and the family Lamiaceae that is widely distributed in southeast Asian countries like India
and China. The plant generally grows in the wet and arid regions of the globe. It has four angled
branches that bear flowers along with heart-shaped leaves. This review aims to provide vast
knowledge about the pharmacological activities, ethnobotany uses, and the phytochemicals present
in the plant. Additionally, it will advance our understanding of the plant's therapeutic potential
in the years to come. Much available literature regarding the pharmacological properties, ethnobotany
uses, and phytochemistry of C .colebrookianium was collected from a series of scientific
resources including PubMed and Google Scholar. Many Asian countries have a long history of using
plants from the Clerodendrum genus for various medicinal purposes. Different extracts of CC
have demonstrated a wide range of pharmacological effects, including those that are antihypertensive,
hepatoprotective, analgesic, antidiabetic and many more. Phytoconstituents like Steroidsdaucosterol,
beta-sitosterol, colebrin-A, colebrin-B, colebrin-C, colebrin-D, colebrin-D and Glycoside-
clerosterol 3 beta-O-[beta-D-glucoside], Clerosterol (24S/3) (poriferasta-5,25(26)-dien-
3-ol, sitosterol and octacosanol were reported to be present in this plant. This article offers a
summary of the most recent research on the pharmacological, traditional uses and some of the phytochemicals
of C. colebrookianium walp. Even though it offers a wide range of medical benefits,
many of its traditional uses for health have not been well researched. The entire therapeutic potential
of these plants for treating a variety of other disorders could be discovered with the aid of in-
-depth research and studies on the phytoconstituents of the plant and their active principles.
Modeling Pressure Effect of Circular Tourniquet Based on Digital Arm
Authors: Qin Yuping , Zhang Man , Kuang Jiangming , Zhang Shuang 
Published: April 2024

Background:
This study aims to investigate displacement deformation of human tissue
in the force region subjected to annular pressure.

Methods:
In this patent, 727 images of a Chinese digital human arm, captured from shoulder to fingertip,
were used as the reconstruction data. The geometric entities of tissue structure were obtained
after tissue segmentation, three-dimensional modeling, and reverse engineering to establish the
working mechanism model of the tourniquet of the human forearm in the finite element simulation
software (COMSOL Multiphysics 5.5). By setting different parameter models (tourniquet pressure
and width models), we analyzed the force conduction mechanism and the displacement deformation
mechanism of the viscoelastic and rigid tissues of the forearm when subjected to annular pressure.

Results:
Modeling analysis showed that when a pressure of 800 kPa was applied on a width of 40
mm, the annular pressure on the viscoelastic tissues was converted into displacement deformation,
thus changing the tissue structure in the body and realizing the hemostatic effect of the tourniquet.
In the case of fixed tourniquet width but variable tourniquet pressure, with the gradual increase of
the pressure, displacement deformation showed an increasing trend. When the externally applied
pressure was fixed and the tourniquet width was different, with the gradual increase of the tourniquet
width, the displacement deformation showed a decreasing trend.

Conclusion:
This patent study demonstrates that both the amount of externally applied pressure and
the width of the tourniquet affect the hemostatic effect of the tourniquet. The hemostatic effect on
the damaged body will be more obvious under a small tourniquet width and large pressure.
Conditional Beta Approximation: Two Applications
Authors: Habibi Reza 
Published: March 2024

Suppose that X,Y are two independent positive continuous random variables. Let P=\frac{X}{X+Y} and Z=X+Y. If X, Y have gamma distributions with the same scale parameter, then P distribution will be beta and P,\ Z are independent. In the case that the distributions of these two variables are not gamma, the P distribution is well approximated by the beta distribution. However, P,\ Z are dependent. According to matching moment method, it is necessary to compute the moments of conditional distribution for beta fitting. In this paper, some new methods for computing moments of conditional distribution of P given Z are proposed. First of all, it is suggested to consider the regression method. Then Monte Carlo simulation is advised. The Bayesian posterior distribution of P is suggested. Applications of differential equations are also reviewed. These results are applied in two applications namely variance change point detection and winning percentage of gambling game are proposed. The probability of change in variance in a sequence of variables, as a leading indicator of possible change, is proposed. Similarly, the probability of winning in a sequential gambling framework is proposed. The optimal time to exit of gambling game is proposed. A game theoretic approach to problem of optimal exit time is proposed. In all cases, beta approximations are proposed. Finally, a conclusion section is also given.
Enabling Health Information Recommendation Using Crowdsourced Refinement in Web-Based Health Information Applications: User-Centered Design Approach and EndoZone Informatics Case Study
Authors: Li Wenhao , O'Hara Rebecca , Hull M Louise , Slater Helen , Sirohi Diksha , Parker Melissa A , Bidargaddi Niranjan 
Published: March 2024

Background
In the digital age, search engines and social media platforms are primary sources for health information, yet their commercial interests–focused algorithms often prioritize irrelevant content. Web-based health applications by reputable sources offer a solution to circumvent these biased algorithms. Despite this advantage, there remains a significant gap in research on the effective integration of content-ranking algorithms within these specialized health applications to ensure the delivery of personalized and relevant health information.

Objective
This study introduces a generic methodology designed to facilitate the development and implementation of health information recommendation features within web-based health applications.

Methods
We detail our proposed methodology, covering conceptual foundation and practical considerations through the stages of design, development, operation, review, and optimization in the software development life cycle. Using a case study, we demonstrate the practical application of the proposed methodology through the implementation of recommendation functionalities in the EndoZone platform, a platform dedicated to providing targeted health information on endometriosis.

Results
Application of the proposed methodology in the EndoZone platform led to the creation of a tailored health information recommendation system known as EndoZone Informatics. Feedback from EndoZone stakeholders as well as insights from the implementation process validate the methodology’s utility in enabling advanced recommendation features in health information applications. Preliminary assessments indicate that the system successfully delivers personalized content, adeptly incorporates user feedback, and exhibits considerable flexibility in adjusting its recommendation logic. While certain project-specific design flaws were not caught in the initial stages, these issues were subsequently identified and rectified in the review and optimization stages.

Conclusions
We propose a generic methodology to guide the design and implementation of health information recommendation functionality within web-based health information applications. By harnessing user characteristics and feedback for content ranking, this methodology enables the creation of personalized recommendations that align with individual user needs within trusted health applications. The successful application of our methodology in the development of EndoZone Informatics marks a significant progress toward personalized health information delivery at scale, tailored to the specific needs of users.
Nutritional Characteristic of Snack bar Formulation Based on Snakehead Fish-Sorghum Flour Flakes and Kidney Beans
Authors: Ma'rifah Bahriyatul , Nurlaily Ari Pebru , Amrullah Adhi Wardhana 
Published: March 2024

Proving supplementary food such as snack bar from high in energy, protein and iron local foods (snakehead fish flour, sorghum and kidney bean) can be used as specific nutrition intervention for CED and anemia in pregnant women. This study aimed to analyze and nutritional characteristic of snakehead fish-sorghum flour flakes and kidney bean snack bar. The experimental method was Completely Randomized Design (CRD) with four ratios of snakehead fish flour-sorghum flakes and kidney beans, namely F1 (50%:50%), F2 (60%:40%), F3 (70%:30%) and F4 (80%:20%). Nutritional characteristic of snack bar was analyzed using One Way ANOVA and Duncan’s further test. The results showed that the difference ratios of snakehead fish flour-sorghum flakes and kidney beans had significant effect on energy, moisture, carbohydrates, fat, protein, zinc, iron and calcium of snack bar (p<0.05). The nutrient content of snack bar F2, F2, F3 and F4 were energy (432,04 kcal; 433,77 kcal; 4329,49 kcal; 437,56 kcal), ash content (3,21%; 3,24%; 3,29%; 3,24%), moisture content (14,46%; 14,71%, 13,35%; 13,72%), fat (20,54%; 21,11%, 19,21%; 21,08%), protein (16,87%; 16,97%; 19,63%; 18,57%), carbohydrates (44,93%; 43,98%; 44,53%; 43,41%), zinc (2,07 mg ; 2,04 mg; 2,07 mg, 2,18 mg), iron (4,35 mg; 6,02 mg; 6,23 mg; 6,76 mg) and calcium (114,21 mg; 130,27 mg; 123,41 mg; 131,83 mg). F4 potentially used as alternative supplementary food for pregnant women with CED and anemia because can be claimed as source of protein and iron product based on food label reference for pregnant women.
Pola Makan dan Status Gizi Anak Sekolah Dasar di SD Inpres 36 Rumah Tiga
Authors: Ruaida Nilfar , Sammeng Wahyuni , Haluruk Merlin K 
Published: March 2024

Anak usia sekolah merupakan kelompok usia yang mengalami proses tumbuh kembang secara pesat, hasil Riskesdas 2018 menunjukan prevalensi status gizi anak sekolah dasar umur 5-12 tahun, dari 9,3 terdiri dari 2,5% sangat kurus dan 6,8% kurus. Di Provinsi Maluku pada umur 5-12 tahun, dari 3,7% sangat kurus dan 11,5% kurus. Pola makan dengan gizi seimbang merupakan perilaku sehat yang berguna untuk memenuhi kebutuhan gizi dan energi anak sekolah. Tujuan penelitian ini untuk mengetahui gambaran pola makan dan status gizi anak sekolah dasar di SD Inpres 36 Rumah Tiga, Ambon. Jenis penelitian adalah penelitian deskriptif. Penentuan sampel menggunakan cara purposive sampling, besar sampel adalah 38 orang. Pengumpulan data tingkat konsumsi makan menggunakan food frequency questionnaire, dan data status gizi menggunakan indeks IMT/U dengan mengukur berat badan dan tinggi badan anak sekolah. Hasil penelitian menunjukkan tingkat konsumsi energi baik sebanyak 21 orang (55%), kurang sebanyak 17 orang (45%), asupan protein baik sebanyak 28 orang (74%), kurang sebanyak 10 orang (26%). Jenis konsumsi makanan kategori baik 36 orang (95%), kurang 2 orang (5%). Frekuensi makan baik 30 (79%), kurang 8 (21%). Status gizi anak sekolah dasar SD Inpres 36 Rumah Tiga, gizi kurang 10 orang (26%), gizi baik 27 orang (71%), dan obesitas 1 orang (3%). Kesimpulan tingkat konsumsi dan status gizi responden, sebagian besar baik.
Potensi Peningkatan Kadar Hemoglobin dengan Pemberian Jus Kurma Bayam Merah pada Remaja Putri
Authors: Masfufah Masfufah , Safitri Safitri , Kariani Ni Ketut 
Published: March 2024

Masalah remaja putri yang paling sering ditemukan adalah anemia. Hal ini dikarenakan remaja putri mengalamai menstruasi setiap bulannya dan masih dalam masa pertumbuhan yang membutuhkan asupan zat besi yang lebih banyak. Berdasarkan data Dinas Kesehatan Provinsi Sulawesi Tengah tahun 2020 di Palu menyatakan bahwa terdapat 16 orang usia 10-14 tahun dan sebanyak 22 orang usia 16-18 tahun mengalami anemia. Adapun tumbuhan dapat menunjang kenaikan kadar hemoglobin antara ain kurma dan bayam merah yang tinggi akan zat besi. Penelitian ini bertujuan untuk menganalisis pengaruh peningkatan kadar hemoglobin terhadap pemberian kombinasi jus kurma bayam merah pada remaja putri anemia. Metode yang digunakan pra-eksperimental one group pra post test design. Teknik sample dengan purposive sampling sebanyak 23 sampel remaja putri dengan variabel independen pemberian jus kurma bayam merah, variabel dependen adalah kadar hemoglobin. Analisis univariat distribusi, frekuensi, persentase. Analisis bivariat menggunakan uji paired sample t test. Hasilnya menunjukkan ada pengaruh jus kurma bayam merah terhadap peningkatan kadar hemoglobin pada remaja putri di SMA Negeri 5 Palu dengan p-value (0,000) (<0,005). Kesimpulan penelitian ini adalah pemberian kombinasi jus kurma bayam merah dapat meningkatkan kadar hemoglobin pada remaja putri di SMA Negeri 5 Palu.
Formulasi Brownies Panggang dengan Substitusi Tepung Daun Kelor dan Tepung Kacang Hijau sebagai Alternatif Cemilan Sumber Zat Besi untuk Remaja Putri Anemia
Authors: Parwati Puput Indah , Ma'rifah Bahriyatul , Muhlishoh Arwin 
Published: March 2024

Pemanfaatan tepung daun kelor dan tepung kacang hijau yang mengandung protein dan zat besi dapat mencegah terjadinya anemia pada remaja putri. Penelitian ini bertujuan untuk mengetahui pengaruh substitusi tepung daun kelor dan tepung kacang hijau terhadap kandungan gizi dan sifat organoleptik brownies panggang. Jenis penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Formulasi brownies panggang terdiri dari 3 perbandingan tepung daun kelor dan tepung kacang hijau yaitu : F1 (25% : 50%), F2 (30% : 55%), F3 (35% : 60%). Data kadar abu, lemak, protein, karbohidrat, zat besi dianalisis dengan uji Anova dan uji lanjut Duncan. Data kadar air dan organoleptik dianalisis dengan uji Kruskal Wallis dan uji lanjut Man Whitney. Hasil penelitian menunjukkan bahwa terdapat pengaruh nyata kandungan gizi dan organoleptik (hedonik: warna, tekstur, mouthfeel, aftertaste, dan mutu hedonik: rasa, mouthfeel, aftertaste). Hasil uji kandungan gizi brownies panggang setiap formulasi (F1, F2, F3) secara berurutan yaitu kadar air 20,29%; 21,03%; 21,87%; kadar abu 3,09%; 2,79%; 2,83%; lemak 12,31%; 13,22%; 12,02%; protein 10,91%; 10,42%; 10,81%; karbohidrat 53,39%; 52,52%; 52,63%; dan zat besi 4,45%; 4,31%; 4,09%. Formulasi terpilih F2 mengandung energi per takaran saji (50 g), yaitu 211,42 kkal, lemak 6,61 g, protein 5,21 g, karbohidrat 26,26 g, dan zat besi 2,15 mg. Brownies panggang F2 memiliki warna hijau tua, beraroma langu, tekstur agak keras, rasa agak enak, mouthfeel agak lembut, dan aftertaste sedang dengan penerimaan organoleptik kurang disukai. Produk sudah memenuhi %AKG remaja putri usia 16–18 tahun dan dapat diklaim sebagai sumber zat besi.