bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermochemical Properties and Decomposition Kinetics of Ammonium Magnesium Phosphate Monohydrate
Authors: Wu Jian , Yuan Ai‐Qun , Huang Zai‐Yin , Tong Zhang‐Fa , Chen Jie , Liang Rong‐Lan 
Published: January 2007

AbstractAmmonium magnesium phosphate monohydrate NH4MgPO4·H2O was prepared via solid state reaction at room temperature and characterized by XRD, FT‐IR and SEM. Thermochemical study was performed by an isoperibol solution calorimeter, non‐isothermal measurement was used in a multivariate non‐linear regression analysis to determine the kinetic reaction parameters. The results show that the molar enthalpy of reaction above is (28.795±0.182) kJ/mol (298.15 K), and the standard molar enthalpy of formation of the title complex is (−2185.43±13.80) kJ/mol (298.15 K). Kinetics analysis shows that the second decomposition of NH4MgPO4·H2O acts as a double‐step reaction:an nth‐order reaction (Fn) with n=4.28, E1=147.35 kJ/mol, A1=3.63×1013 s−1 is followed by a second‐order reaction (F2) with E2=212.71 kJ/mol, A2=1.82×1018 s−1.
A Convenient Synthesis of Novel Meldrum's Acid C60 Fullerene Derivatives
Authors: Jiang Hu , Zhang Jian‐Min , Du Wei‐Qiong , Zhu Shi‐Zheng 
Published: January 2007

AbstractA series of novel Meldrum′s acid C60 derivatives were prepared in moderate yields from a convenient one‐pot reaction of C60, the Meldrum's acid derivatives, I2 and 1,8‐diazabicyclo‐[5,4,0]‐undec‐7‐ene (DBU) in toluene at room temperature under nitrogen atmosphere. All the new compounds were fully characterized by the spectral data and elemental analysis. A carbene intermediate mechanism was proposed for this reaction.
A Study on the Reaction of 1‐(3‐Pyridyl)‐2,2‐di‐substituted Ethylenes with 1‐Benzyl‐1,4‐dihydronicotinamide
Authors: Xu Hua‐Jian , Liu You‐Cheng , Fu Yao 
Published: January 2007

AbstractThe reaction of a series of 1‐(3‐pyridyl)‐2,2‐di‐substituted ethylenes with 1‐benzyl‐1,4‐dihydronicotinamide (BNAH) in deaerated acetonitrile produces the corresponding 1‐(3‐pyridyl)‐2,2‐di‐substituted ethanes in contrast to benzylidenemalononitrile (BM) which does not react with BNAH under the same conditions.
Derivatives of Iressa, a Specific Epidermal Growth Factor Receptor Inhibitor, are Powerful Apoptosis Inducers in PC3 Prostatic Cancer Cells
Authors: Telliez Aurélie , Desroses Matthieu , Pommery Nicole , Briand Olivier , Farce Amaury , Laconde Guillaume , Lemoine Amélie , Depreux Patrick , Hénichart Jean‐Pierre 
Published: January 2007

AbstractThe tyrosine kinase activity of the epidermal growth factor receptor (EGFR) is widely involved in signaling pathways and often deregulated in cancer. Its role in the development of prostate cancer is well established, and therapeutic strategies such as blockade of the intracellular tyrosine kinase domain with small‐molecule tyrosine kinase inhibitors have been proposed. Herein we describe the synthesis and in vitro pharmacological properties of C6‐ and C7‐substituted 4‐anilinoquinazolines, analogues of Iressa and powerful proapoptotic inducers in hormone‐independent prostate cancer PC3 cell lines.
Prospective study of adiposity and weight change in relation to prostate cancer incidence and mortality
Authors: Wright Margaret E. , Chang Shih‐Chen , Schatzkin Arthur , Albanes Demetrius , Kipnis Victor , Mouw Traci , Hurwitz Paul , Hollenbeck Albert , Leitzmann Michael F. 
Published: January 2007

AbstractBACKGROUND.Adiposity has been linked inconsistently with prostate cancer, and few studies have evaluated whether such associations vary by disease aggressiveness.METHODS.The authors prospectively examined body mass index (BMI) and adult weight change in relation to prostate cancer incidence and mortality in 287,760 men ages 50 years to 71 years at enrollment (1995–1996) in the National Institutes of Health‐AARP Diet and Health Study. At baseline, participants completed questionnaires regarding height, weight, and cancer screening practices, including digital rectal examinations and prostate‐specific antigen tests. Cox regression analysis was used to calculate relative risks (RR) and 95% confidence intervals (95% CIs).RESULTS.In total, 9986 incident prostate cancers were identified during 5 years of follow‐up, and 173 prostate cancer deaths were ascertained during 6 years of follow‐up. In multivariate models, higher baseline BMI was associated with significantly reduced total prostate cancer incidence, largely because of the relationship with localized tumors (for men in the highest BMI category [≥40 kg/m2] vs men in the lowest BMI category [<25 kg/m2]: RR, 0.67; 95% CI, 0.50–0.89; P = .0006). Conversely, a significant elevation in prostate cancer mortality was observed at higher BMI levels (BMI <25 kg/m2: RR, 1.0 [referent group]; BMI 25–29.9 kg/m2: RR, 1.25; 95% CI, 0.87–1.80; BMI 30–34.9 kg/m2: RR, 1.46; 95% CI, 0.92–2.33; and BMI ≥35 kg/m2: RR, 2.12; 95% CI, 1.08–4.15; P = .02). Adult weight gain from age 18 years to baseline also was associated positively with fatal prostate cancer (P = .009), but not with incident disease.CONCLUSIONS.Although adiposity was not related positively to prostate cancer incidence, higher BMI and adult weight gain increased the risk of dying from prostate cancer. Cancer 2007. Published 2007 by the American Cancer Society.
A pooled analysis of quality of life measures and adverse events data in north central cancer treatment group lung cancer clinical trials
Authors: Huschka Mashele M. , Mandrekar Sumithra J. , Schaefer Paul L. , Jett James R. , Sloan Jeff A. 
Published: January 2007

AbstractBACKGROUND.In this pooled analysis, the authors examined correlations between single‐item and multiple‐item quality of life (QOL) measures and assessed the agreement between clinically significant changes in QOL and patient‐reported adverse events (AE).METHODS.Data from 6 lung cancer clinical trials that involved 358 patients were pooled. All trials incorporated the Uniscale and 1 of 3 multiple‐item assessments: the Functional Assessment for Cancer Therapy‐Lung, the Lung Cancer Symptom Scale, or the Symptom Distress Scale. Spearman rank correlations and a Bland‐Altman approach were used to assess agreement. Time‐to‐event analysis was performed using the Kaplan‐Meier method.RESULTS.Correlations between the Uniscale and multiple‐item assessments were substantial (correlation coefficient = 0.49–0.66). At least 1 10‐point decline was reported in the Uniscale and multiple‐item assessments by 58% of patients and 39% of patients, respectively. At least 1 severe AE (grade ≥3) was reported in 35% of patients postbaseline. The percent agreement between experiencing a severe AE and a decline in QOL was 48% and 59% for the Uniscale and multiple‐item assessments, respectively. The median time to the first 10‐point decline in QOL for the Uniscale and multiple‐item assessments was 67 days and 142 days, respectively, and the median time to the first occurrence of a severe AE was 304 days.CONCLUSIONS.Information gleaned from the single‐item Uniscale assessment was comparable to that gleaned from multiple‐item global measures. There was moderate agreement between QOL and AE. A 10‐point decline in QOL occurred earlier than Common Toxicity Criteria AE reporting. This suggests the need for inclusion of a QOL instrument in lung cancer clinical trials. Cancer 2007. © 2007 American Cancer Society.
Expression of epidermal growth factor receptor in esophageal and esophagogastric junction adenocarcinomas
Authors: Wang Kim L. , Wu Tsung‐Teh , Choi In Seon , Wang Huamin , Resetkova Erika , Correa Arlene M. , Hofstetter Wayne L. , Swisher Stephen G. , Ajani Jaffer A. , Rashid Asif , Albarracin Constance T. 
Published: January 2007

AbstractBACKGROUND.The prognosis for patients with esophageal and esophagogastric junction (EGJ) adenocarcinoma remains poor, even after surgical resection. Pathologic assessment of depth of invasion and lymph node status are the primary prognostic factors in these patients. In patients with esophageal squamous cell carcinoma, increased epidermal growth factor receptor (EGFR) expression has been associated with a worse prognosis. It is not known whether EGFR plays a similar role in esophageal and EGJ adenocarcinomas.METHODS.To address this issue, the authors studied tumor specimens from 103 patients with surgically resected esophageal and EGJ adenocarcinomas (9 patients with stage I disease, 23 patients with stage II disease, 57 patients with stage III disease, and 14 patients with stage IV disease). The expression of EGFR was assessed by immunohistochemical analysis of tissue microarrays. Tumors were considered positive for EGFR expression when >5% of tumor cells were stained and negative when ≤5% of tumor cells were stained.RESULTS.EGFR was expressed in 33 of 103 adenocarcinomas (32%) and was correlated with higher pathologic tumor (T) classification (P = .02), the presence of lymph node metastasis (P = .01), and higher pathologic tumor, lymph node, metastasis classification (P = .02). EGFR expression also was correlated with shorter disease‐free and overall survival in univariate analyses (P = .001 and P = .004, respectively), and there was a trend toward a correlation between EGFR expression and shorter disease‐free survival in multivariate analyses (P = .07 and P = .08). The results demonstrated that EGFR expression in esophageal adenocarcinomas was correlated with advanced pathologic tumor classification and lymph node metastasis. EGFR expression also was correlated with poor disease‐free and overall survival, but that correlation was not independent of T classification.CONCLUSIONS.The current findings suggested that EGFR expression correlates with poor prognostic factors and may be used to predict patient outcomes. Cancer 2007. © 2007 American Cancer Society.
Randomized phase II trial of two different schedules of docetaxel plus cisplatin as first‐line therapy in advanced nonsmall cell lung cancer
Authors: Park Se Hoon , Choi Soo Jin , Kyung Sun Young , An Chang Hyeok , Lee Sang Pyo , Park Jeong Woong , Jeong Sung Hwan , Cho Eun Kyung , Shin Dong Bok , Hoon Lee Jae 
Published: January 2007

AbstractBACKGROUND.There is increasing interest in the use of a weekly administration of docetaxel as a way of reducing its hematologic toxicity. The purpose of the current randomized study was to evaluate the toxicity and efficacy of docetaxel plus cisplatin combination on 2 schedules in patients with previously untreated, advanced nonsmall‐cell lung cancer (NSCLC).METHODS.Consenting patients with advanced NSCLC were randomized to receive first‐line chemotherapy with cisplatin 75 mg/m2 on Day 1, plus 3‐weekly (75 mg/m2 on Day 1) or weekly (35 mg/m2 on Days 1, 8, and 15 of a 4‐week cycle) docetaxel, for up to 6 cycles.RESULTS.Of 86 patients accrued, 41 patients were treated with 3‐weekly and 43 with weekly docetaxel plus cisplatin. The most frequent grade 3/4 toxicity in the 3‐weekly arm was neutropenia (56% of patients). In those receiving the weekly regimen, the frequent grade 3/4 toxicities were fatigue (44%) and nausea/vomiting (35%). The overall response rate was 40% with the 3‐weekly and 39% with the weekly arm (P = .74). The median progression‐free survival was 4.3 months in the 3‐weekly arm and 3.9 months in the weekly arm (P = .08) and the median survival was 10.3 and 10.0 months, respectively (P = .76). Quality of life data showed no relevant difference between the arms.CONCLUSIONS.The weekly schedule of docetaxel plus cisplatin combination as first‐line chemotherapy for advanced NSCLC, while feasible, has no clear advantage over the standard 3‐weekly regimen. Cancer 2007. © 2007 American Cancer Society.
Flow cytometry to sort mammalian cells in cytokinesis
Authors: Gasnereau Isabelle , Ganier Olivier , Bourgain Florence , Gramont Armand de , Gendron Marie‐Claude , Sobczak‐Thépot Joëlle 
Published: January 2007

AbstractBackground:Cell division or cytokinesis, which results from a series of events starting in metaphase, is the mechanism by which the mother cell cytoplasm is divided between the two daughter cells. Hence it is the final step of the cell division cycle. The aim of the present study was to demonstrate that mammalian cells undergoing cytokinesis can be sorted selectively by flow cytometry.Materials and Methods:Cultures of HeLa cells were arrested in prometaphase by nocodazole, collected by mitotic shake‐off and released for 90 min into fresh medium to enrich for cells undergoing cytokinesis. After ethanol fixation and DNA staining, cells were sorted based on DNA content and DNA fluorescence signal height.Results:We define a cell population that transiently accumulates when synchronized cells exit mitosis before their entry into G1. We show that this population is highly enriched in cells undergoing cytokinesis. In addition, this population of cells can be sorted and analyzed by immunofluorescence and western blotting.Conclusions:This method of cell synchronization and sorting provides a simple means to isolate and biochemically analyze cells in cytokinesis, a period of the cell cycle that has been difficult to study by cell fractionation. © 2007 International Society for Analytical Cytology
Combination of automated high throughput platforms, flow cytometry, and hierarchical clustering to detect cell state
Authors: Kitsos Christine M. , Bhamidipati Phani , Melnikova Irena , Cash Ethan P. , McNulty Chris , Furman Julia , Cima Michael J. , Levinson Douglas 
Published: January 2007

AbstractBackground:This study examined whether hierarchical clustering could be used to detect cell states induced by treatment combinations that were generated through automation and high‐throughput (HT) technology. Data‐mining techniques were used to analyze the large experimental data sets to determine whether nonlinear, non‐obvious responses could be extracted from the data.Methods:Unary, binary, and ternary combinations of pharmacological factors (examples of stimuli) were used to induce differentiation of HL‐60 cells using a HT automated approach. Cell profiles were analyzed by incorporating hierarchical clustering methods on data collected by flow cytometry. Data‐mining techniques were used to explore the combinatorial space for nonlinear, unexpected events. Additional small‐scale, follow‐up experiments were performed on cellular profiles of interest.Results:Multiple, distinct cellular profiles were detected using hierarchical clustering of expressed cell‐surface antigens. Data‐mining of this large, complex data set retrieved cases of both factor dominance and cooperativity, as well as atypical cellular profiles. Follow‐up experiments found that treatment combinations producing “atypical cell types” made those cells more susceptible to apoptosis.Conclusions:Hierarchical clustering and other data‐mining techniques were applied to analyze large data sets from HT flow cytometry. From each sample, the data set was filtered and used to define discrete, usable states that were then related back to their original formulations. Analysis of resultant cell populations induced by a multitude of treatments identified unexpected phenotypes and nonlinear response profiles. © 2007 International Society for Analytical Cytology
Chromatin supraorganization and extensibility in mouse hepatocytes with development and aging
Authors: Moraes Alberto S. , Guaraldo Ana Maria A. , Mello Maria Luiza S. 
Published: January 2007

AbstractBackground:Chromatin supraorganization and extensibility, which lead to the formation of extended chromatin fibers (ECF), are affected by starvation and refeeding in adult mouse hepatocytes. It is expected that they could also change with mouse development and aging.Methods:Methods used involved topochemistry, image analysis, microspectrophotometry, gravity action, and polarization microscopy.Results:Increased nuclear areas and Feulgen‐DNA amounts with advancing hepatocyte polyploidy were found with development and aging. A slightly less packed chromatin with more heterogeneously distributed condensation levels was detected in young and old mice. Con‐A responsiveness was almost absent in young mice but very deep in aged mice. ECFs formed from nuclei of adult and aged mice but not from nuclei of young mice. The frequency of ECF formation with the long lysis protocol increased with aging.Conclusions:In young mice, a less packed chromatin state may be associated with more intense gene activity, thus increasing the DNA‐nuclear matrix interactions, and inhibiting ECF formation. Reduced DNA‐nuclear matrix interactions besides defects in heterochromatin formation may induce higher ECF formation and chromatin unpackaging in old mice. We suggest that differences in Con‐A staining relate to different gene activity with advancing development and aging. © 2007 International Society for Analytical Cytology
Rearrangement of a conditional allele regardless of inheritance of a Cre recombinase transgene
Authors: Cochrane Rebecca L. , Clark Stephen H. , Harris Adam , Kream Barbara E. 
Published: January 2007

AbstractTo generate conditional gene knockouts in osteoblasts, we previously developed transgenic mice in which Cre recombinase cDNA was cloned downstream of a 3.6 or 2.3 kb fragment of the rat Col1a1 promoter (Col3.6‐Cre and Col2.3‐Cre, respectively). Col‐Cre mice were bred with mice in which exon 4 of the Igf1 gene is flanked by loxP sites. Mating units were arranged such that either the male or the female breeder transmitted the Col‐Cre transgenes. Progeny were evaluated for Cre‐mediated Igf1 gene rearrangement. We found that the loxP‐flanked Igf1 locus was rearranged in the absence of inheritance of the Cre transgene. The incidence was 50 and 28% with Col2.3‐Cre and Col3.6‐Cre females, respectively, and 15 and 18% with Col2.3‐Cre and Col3.6‐Cre males, respectively. genesis 45:17–20, 2007. © 2007 Wiley‐Liss, Inc.
Generation of a transgenic mouse model with chondrocyte‐specific and tamoxifen‐inducible expression of Cre recombinase
Authors: Chen Mo , Lichtler Alexander C. , Sheu Tzong‐Jen , Xie Chao , Zhang Xinping , O'Keefe Regis J. , Chen Di 
Published: January 2007

AbstractPostnatal cartilage development and growth are regulated by key growth factors and signaling molecules. To fully understand the function of these regulators, an inducible and chondrocyte‐specific gene deletion system needs to be established to circumvent the perinatal lethality. In this report, we have generated a transgenic mouse model (Col2a1‐CreERT2) in which expression of the Cre recombinase is driven by the chondrocyte‐specific col2a1 promoter in a tamoxifen‐inducible manner. To determine the specificity and efficiency of the Cre recombination, we have bred Col2a1‐CreERT2 mice with Rosa26R reporter mice. The X‐Gal staining showed that the Cre recombination is specifically achieved in cartilage tissues with tamoxifen‐induction. In vitro experiments of chondrocyte cell culture also demonstrate the 4‐hydroxy tamoxifen‐induced Cre recombination. These results demonstrate that Col2a1‐CreERT2 transgenic mice can be used as a valuable tool for an inducible and chondrocyte‐specific gene deletion approach. genesis 45:44–50, 2007. Published 2007 Wiley‐Liss, Inc.
An allelic series for studying the mouse Thoc1 gene
Authors: Wang Xiaoling , Li Yanping , Zhang Xiaojing , Goodrich David W. 
Published: January 2007

AbstractThoc1 encodes an essential component of the mammalian TREX protein complex. TREX is an evolutionary conserved complex that couples elongating RNA polymerase II with RNA processing factors. Depletion of Thoc1 protein (pThoc1) compromises transcriptional elongation and nuclear export of some RNAs. Loss of Thoc1 causes periimplantation embryonic lethality in the mouse. Early embryonic lethality precludes analysis of the physiological requirements for Thoc1 in the developing embryo or adult. To circumvent this limitation, we have generated mice containing hypomorphic or conditional alleles of Thoc1. Mice homozygous for the conditional allele appear normal. Mice containing Cre recombined conditional alleles phenocopy the previously characterized Thoc1 null allele. Mice homozygous for the hypomorphic allele are viable and born at a frequency that is not significantly different from the expected Mendelian ratio. However, these mice express less pThoc1 than wild type mice and exhibit a dwarf phenotype. The dwarf phenotype can be detected in mid‐gestation embryos, suggesting that Thoc1 is also required later in embryonic and postnatal development. genesis 44:32–37, 2007. Published 2007 Wiley‐Liss, Inc.
Birefringence generation mechanism in a fiber Fabry‐Perot etalon buried in a fiber connector housing
Authors: Tateda Mitsuhiro , Otake Shizuka , Shintaku Toshihiro , Omatsu Takashige 
Published: January 2007

AbstractWe derived an analytical model according to which birefringence in a fiber Fabry‐Perot etalon occurs due to the anisotropy of adhesive layers in the cementing of the fiber to the ferrule. We found that the birefringence was proportional to the product of the difference in the coefficients of linear expansion of the fiber and ferrule materials, the change in temperature, the Young's modulus of the adhesive, and the anisotropy of the adhesive layer thickness. In addition, by measuring the birefringence of six fiber Fabry‐Perot etalons fabricated using three types of adhesives that differed in curing shrinkage and viscosity, we showed that the birefringence has a positive correlation with the curing shrinkage of the adhesive used. The measured temperature dependence of birefringence was consistent with the order of magnitude of the value calculated using the derived model, confirming the validity of the proposed model. © 2007 Wiley Periodicals, Inc. Electron Comm Jpn Pt 1, 90(5): 56–62, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecja.20174
Characteristics of biological tissue equivalent phantoms applied to UWB communications
Authors: Takimoto Takuya , Onishi Teruo , Saito Kazuyuki , Takahashi Masaharu , Uebayashi Shinji , Ito Koichi 
Published: January 2007

AbstractPreviously, the authors studied biological tissue equivalent phantoms able to simulate the electrical constants of the human body in the 3‐ to 6‐GHz band in a single composition ratio. Therefore, in this paper, we examined how to study the antenna characteristics using the phantom when extended to a bandwidth including 900 MHz to 3 GHz and 6 to 10 GHz and performed a quantitative study focusing on the electrical constants of the phantom. The result clearly showed hardly any effect of divergence of the phantom's electrical constants from the target values on the antenna input impedance, radiation efficiency, and radiation directivity. Therefore, in the entire ultra‐wideband (UWB), this phantom can be accurately evaluated through antenna characteristic measurements and is clearly effective. Furthermore, differences due to the divergence of the phantom's electrical constants from the target values in the entire UWB bandwidth (3 to 10 GHz) are within ±3% for the average local specific absorption rate (SAR) inside the phantom caused by the electromagnetic wave energy radiated from the antenna. © 2007 Wiley Periodicals, Inc. Electron Comm Jpn Pt 1, 90(5): 48–55, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecja.20300
Fragmentation of the (Cyclam‐acetato)iron Azide Cation in the Gas Phase
Authors: Schröder Detlef , Schwarz Helmut , Aliaga‐Alcalde Núria , Neese Frank 
Published: January 2007

AbstractMass spectrometry is used to investigate the fragmentation of the ligated azidoiron cation [(cyclam‐acetato)Fe(N3)]+, which is accessible in the gas phase by electrospray ionization of a solution of its hexafluorophosphate salt in methanol/water. Upon collisional activation, mass‐selected [(cyclam‐acetato)Fe(N3)]+ undergoes competing loss of dinitrogen or HN3 as the prevailing fragmentations. The former dissociation pathway is investigated in detail in order to determine whether or not the free, high‐valent iron nitride [(cyclam‐acetato)FeN]+ is formed. The evidence obtained indeed supports the formation of the iron nitride species as an intermediate, although the long‐lived ion sampled after mass selection may also have undergone further rearrangements.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)