bluuebunny/crossref_metadata_2025_split huggingface dataset:
Delineating redox cooperativity in water-soluble and membrane multiheme cytochromes through protein design
Authors: Hardy Benjamin J. , Dubiel Paulina , Bungay Ethan L. , Rudin May , Williams Christopher , Arthur Christopher J. , Guberman-Pfeffer Matthew J. , Oliveira A. Sofia , Curnow Paul , Anderson J. L. Ross 
Published: March 2024

AbstractNature has evolved diverse electron transport proteins and multiprotein assemblies essential to the generation and transduction of biological energy. However, substantially modifying or adapting these proteins for user-defined applications or to gain fundamental mechanistic insight can be hindered by their inherent complexity.De novoprotein design offers an attractive route to stripping away this confounding complexity, enabling us to probe the fundamental workings of these bioenergetic proteins and systems, while providing robust, modular platforms for constructing completely artificial electron-conducting circuitry. Here, we use a set ofde novodesigned mono-heme and di-heme soluble and membrane proteins to unpick the contributions of electrostatic micro-environments and dielectric properties of the surrounding protein medium on the inter-heme redox cooperativity that we have previously reported. Experimentally, we find that the two heme sites in both the water-soluble and membrane constructs have broadly equivalent redox potentials in isolation, in agreement with Poisson-Boltzmann Continuum Electrostatics calculations. BioDC, a Python program for the estimation of electron transfer energetics and kinetics within multiheme cytochromes, also predicts equivalent heme sites, and reports that burial within the low dielectric environment of the membrane strengthens heme-heme electrostatic coupling. We conclude that redox cooperativity in our diheme cytochromes is largely driven by heme electrostatic coupling and confirm that this effect is greatly strengthened by burial in the membrane. These results demonstrate that while ourde novoproteins present minimalist, new-to-nature constructs, they enable the dissection and microscopic examination of processes fundamental to the function of vital, yet complex, bioenergetic assemblies.
The L-lactate dehydrogenases ofPseudomonas aeruginosaare conditionally regulated but both contribute to survival during macrophage infection
Authors: Florek Lindsey C. , Lin Xi , Lin Yu-Cheng , Lin Min-Han , Chakraborty Arijit , Price-Whelan Alexa , Tong Liang , Rahme Laurence , Dietrich Lars E.P. 
Published: March 2024

AbstractPseudomonas aeruginosais an opportunistic pathogen that thrives in environments associated with human activity, including soil and water altered by agriculture or pollution. Because L-lactate is a significant product of plant and animal metabolism, it is available to serve as a carbon source forP. aeruginosain the diverse settings it inhabits. Here, we evaluateP. aeruginosa’s production and use of its redundant L-lactate dehydrogenases, termed LldD and LldA. We confirm that the protein LldR represseslldDand identify a new transcription factor, called LldS, that activateslldA; these distinct regulators and the genomic contexts oflldDandlldAcontribute to their differential expression. We demonstrate that thelldDandlldAgenes are conditionally controlled in response to lactate isomers as well as to glycolate and ◻-hydroxybutyrate, which, like lactate, are ◻-hydroxycarboxylates. We also show thatlldAis induced when iron availability is low. Our examination oflldDandlldAexpression across depth in biofilms indicates a complex pattern that is consistent with the effects of glycolate production, iron availability, and cross-regulation on enzyme preference. Finally, macrophage infection assays revealed that bothlldDandlldAcontribute to persistence within host cells, underscoring the potential role of L-lactate as a carbon source duringP. aeruginosa-eukaryote interactions. Together, these findings help us understand the metabolism of a key resource that may promoteP. aeruginosa’s success as a resident of contaminated environments and animal hosts.ImportancePseudomonas aeruginosais a major cause of lung infections in people with cystic fibrosis, hospital-acquired infections, and wound infections. It consumes L-lactate, which is found at substantial levels in human blood and tissues. In this study, we investigated the spatial regulation of two redundant enzymes, called LldD and LldA, which enable L-lactate metabolism inP. aeruginosabiofilms. We uncovered mechanisms and identified compounds that controlP. aeruginosa’s LldD/LldA preference. We also showed that both enzymes contribute to its ability to survive within macrophages, a behavior that is thought to augment the chronicity and recalcitrance of infections. Our findings shed light on a key metabolic strategy used byP. aeruginosaand have the potential to inform the development of therapies targeting bacterial metabolism during infection.
Identification and functional analysis of recent IS transposition events in rhizobia
Authors: Mogro Ezequiel G. , Draghi Walter O. , Lagares Antonio , Lozano Mauricio J. 
Published: March 2024

ABSTRACTRhizobia are alpha- and betaproteobacteria that, through the establishment of symbiotic interactions with leguminous plants, are able to fix atmospheric nitrogen as ammonium. The successful establishment of a symbiotic interaction is highly dependent on the availability of nitrogen sources in the soil, and on the specific rhizobia strain. Insertion sequences (ISs) are simple transposable genetic elements that can move to different locations within the host genome and are known to play an important evolutionary role, contributing to genome plasticity by acting as recombination hot-spots, and disrupting coding and regulatory sequences. Disruption of coding sequences may have occurred either in a common ancestor of the species or more recently. By means of ISComapare, we identified Differentially Located ISs (DLIS) in nearly related rhizobial strains of the generaBradyrhizobium, Mesorhizobium, RhizobiumandSinorhizobium. Our results revealed that recent IS transposition events don’t seem to be playing a major role in adaptation. Nevertheless, DLIS could have a role enabling the activation and inactivation of certain genes that could dynamically affect the competition and survival of rhizobia in the rhizosphere.
Dark Matter Induced Power in Quantum Devices
Authors: Das Anirban , Kurinsky Noah , Leane Rebecca K. 
Published: March 2024

We point out that power measurements of single quasiparticle devices open a new avenue to detect dark matter (DM). The threshold of these devices is set by the Cooper pair binding energy, and is therefore so low that they can detect DM as light as about an MeV incoming from the Galactic halo, as well as the low-velocity thermalized DM component potentially present in the Earth. Using existing power measurements with these new devices, as well as power measurements with SuperCDMS-CPD, we set new constraints on the spin-independent DM scattering cross section for DM masses from about 10 MeV to 10 GeV. We outline future directions to improve sensitivity to both halo DM and a thermalized DM population in the Earth using power deposition in quantum devices.

Published by the American Physical Society
2024
Photoelectron Circular Dichroism in the Spin-Polarized Spectra of Chiral Molecules
Authors: Artemyev A. N. , Tomar R. , Trabert D. , Kargin D. , Kutscher E. , Schöffler M. S. , Schmidt L. Ph. H. , Pietschnig R. , Jahnke T. , Kunitski M. , Eckart S. , Dörner R. , Demekhin Ph. V. 
Published: March 2024

We studied strong-field multiphoton ionization of 1-iodo-2-methylbutane enantiomers with 395 nm circularly polarized laser pulses experimentally and theoretically. For randomly oriented molecules, we observe spin polarization up to about 15%, which is independent of the molecular enantiomer. Our experimental findings are explained theoretically as an intricate interplay between three contributions from HOMO, HOMO-1, and HOMO-2, which are formed of 5p-electrons of the iodine atom. For uniaxially oriented molecules, our theory demonstrates even larger spin polarization. Moreover, we predict a sizable enantiosensitive photoelectron circular dichroism of about 10%, which is different for different spin states of photoelectrons.

Published by the American Physical Society
2024
Why do knowledge seekers turn to knowledge contributors in social Q&A platforms? Evidence from China
Authors: Qiu Junping , Mi Qinze , Xu Zhongyang , Zhang Tingyong , Zhou Tao 
Published: March 2024

PurposeBased on the social interaction theory and trust theory, this study investigates the switching of users on social question and answer (Q&A) platforms from knowledge seekers to knowledge contributors.Design/methodology/approachWe used Python to gather data from Zhihu, performed hypothesis testing on the models using Poisson regression and finally conducted a mediation effect analysis.FindingsThe findings reveal that knowledge seeking impacts users' motivation for information interaction, emotional interaction and trust. Notably, information interaction and trust exhibit a chained mediation effect that subsequently influences knowledge contribution.Originality/valueCurrent studies on user knowledge behavior typically examine individual actions, rarely connecting knowledge seeking and knowledge contribution. However, the balance of knowledge inflow and outflow is crucial for social Q&A platforms. To cover this gap, this paper empirically investigates the switching between knowledge seeking and knowledge contribution based on the social interaction theory and trust theory.
When digitalization meets HRM: developing a HRM value chain model in China
Authors: Zhao Yixuan , He Guangyuan , Wei Danxia , Zhao Shuming 
Published: March 2024

Purpose
The purpose of this study is to explore the mechanism of digitalized transformation in organizations’ human resource management (HRM). This study summarizes three basic factors driving the digital transformation process in China: level of perception, level of application and speed of transformation.

Design/methodology/approach
This study analyzes the strategic transformation process of HRM in Haier, Hisense and Chambroad to explore the human resource digital transformation mechanism in Chinese enterprises.

Findings
The results of this study show that three HR value chain models can be constructed based on how well HRM deals with business: the efficiency-oriented HRM value chain, quasi-business-oriented HRM value chain and business-oriented HRM value chain. The basic factors – level of perception, level of application and speed of transformation – are observed in the entire HRM digital transformation process.

Originality/value
This study provides theoretical and empirical insights for enterprises to explore the value of digital technology in HRM and facilitate the digital transformation of HRM.
Stakeholder exclusion practices of responsible leaders: an investigation into the application of responsible leader values in stakeholder inclusion and exclusion
Authors: Ellis Mark , Dean Dianne 
Published: March 2024

PurposeThe aim of this paper is to explore the stakeholder exclusion practices of responsible leaders.Design/methodology/approachAn interpretive multiple case analyses of seven responsibly led organisations was employed. Twenty-two qualitative interviews were undertaken to investigate and understand perceptions and practice of responsible leaders and their approach to stakeholder inclusion and exclusion.FindingsThe findings revealed new and surprising insights where responsible leaders compromised their espoused values of inclusivity through the application of a personal bias, resulting in the exclusion of certain stakeholders. This exclusivity practice focused on the informal evaluation of potential stakeholders’ values, and where they did not align with those of the responsible leader, these stakeholders were excluded from participation with the organisation. This resulted in the creation and continuity of a culture of shared moral purpose across the organisation.Research limitations/implicationsThis study focussed on responsible leader-led organisations, so the next stage of the research will include mainstream organisations (i.e. without explicit responsible leadership) to examine how personal values bias affects stakeholder selection in a wider setting.Practical implicationsThe findings suggest that reflexive practice and critically appraising management methods in normative leadership approaches may lead to improvements in diversity management.Originality/valueThis paper presents original empirical data challenging current perceptions of responsible leader inclusivity practices and indicates areas of leadership development that may need to be addressed.
Implications of remote work in post-pandemic times: a gender risk profile in Colombian workers
Authors: Campos García Ángela Ximena , Cabrera-García Victoria Eugenia , Docal-Millán María del Carmen , Acuña Arango Lina María , Riveros Munevar Fernando 
Published: March 2024

Purpose
Remote work has been intensifying in organizations, and the recent pandemic forced an immediate increase in it, ignoring its effect on the family. The purpose of this study was to analyze the work and personal-family life balance of Colombian workers during the lockdown and the effects on post-pandemic times.

Design/methodology/approach
Quantitative correlational study with a non-probabilistic sample of 1,069 participants: 349 (32.64%) men and 720 (67.35%) women.

Findings
A total of 44.8% of the participants reported that their work interfered with their personal life; 61.6% reported that their work exceeded their habitual time; 72.2% felt comfortable with the remote work; and women perceived more affectation, as did participants with children. No interaction was present between these variables. There are more interruptions for workers with children younger than 12 years.

Practical implications
There is satisfaction with remote work. However, there are difficulties regarding work-personal life balance that must be addressed to improve quality of life, with an emphasis on women and workers with children, especially younger children.

Social implications
This study provides empirical evidence for the foundation of public and organizational policies aimed at managing remote work and the work-personal life balance to reduce the risk of loss of female labor force and effects on the quality of life of workers.

Originality/value
Studies on the work-personal life balance with Latin American samples are scarce. This research contributes to the literature about satisfaction with working from home modality and the work-personal life balance during COVID-19 confinement, with a look at the differences by gender and the evaluation of the family conditions of Colombian workers, contributing to a regional perspective.
Boron nitride doped with transition metals for carbon monoxide detection: a promising nanosensor for air cleaning
Authors: Mollaamin Fatemeh , Monajjemi Majid 
Published: March 2024

Purpose
This study aims to investigate the potential of the decorated boron nitride nanocage (BNNc) with transition metals for capturing carbon monoxide (CO) as a toxic gas in the air.

Design/methodology/approach
BNNc was modeled in the presence of doping atoms of titanium (Ti), vanadium (V), chromium (Cr), cobalt (Co), copper (Cu) and zinc (Zn) which can increase the gas sensing ability of BNNc. In this research, the calculations have been accomplished by CAM–B3LYP–D3/EPR–3, LANL2DZ level of theory. The trapping of CO molecules by (Ti, V, Cr, Co, Cu, Zn)–BNNc has been successfully incorporated because of binding formation consisting of C → Ti, C → V, C → Cr, C → Co, C → Cu, C → Zn.

Findings
Nuclear quadrupole resonance data has indicated that Cu-doped or Co-doped on pristine BNNc has high fluctuations between Bader charge versus electric potential, which can be appropriate options with the highest tendency for electron accepting in the gas adsorption process. Furthermore, nuclear magnetic resonance spectroscopy has explored that the yield of electron accepting for doping atoms on the (Ti, V, Cr, Co, Cu, Zn)–BNNc in CO molecules adsorption can be ordered as follows: Cu > Co >> Cr > Zn ˜ V> Ti that exhibits the strength of the covalent bond between Ti, V, Cr, Co, Cu, Zn and CO. In fact, the adsorption of CO gas molecules can introduce spin polarization on the (Ti, V, Cr, Co, Cu, Zn)–BNNc which specifies that these surfaces may be used as magnetic-scavenging surface as a gas detector. Gibbs free energy based on IR spectroscopy for adsorption of CO molecules adsorption on the (Ti, V, Cr, Co, Cu, Zn)–BNNc have exhibited that for a given number of carbon donor sites in CO, the stabilities of complexes owing to doping atoms of Ti, V, Cr, Co, Cu, Zn can be considered as: CO →Cu–BNNc >> CO → Co–BNNc > CO → Cr–BNNc > CO → V–BNNc > CO → Zn–BNNc > CO → Ti–BNNc.

Originality/value
This study by using materials modeling approaches and decorating of nanomaterials with transition metals is supposed to introduce new efficient nanosensors in applications for selective sensing of carbon monoxide.
“They will at least do no harm”: LGBTQ social studies teacher education
Authors: Brant Cathy A.R. 
Published: March 2024

PurposeThis study aims to explore the self-efficacy of social studies teacher education working with lesbian, gay, bisexual, transgender and queer (LGBTQ) preservice teachers, teaching LGBTQ content in their methods courses, and helping the preservice teachers they teach in those classes reduce their bias and prejudice against LGBTQ individuals.Design/methodology/approachThis study, framed by self-efficacy theory, employs a mixed methods approach, qualitative semistructured interviews (n = 6) and quantitative (Likert-scale) survey questions (n = 174).FindingsParticipants reported high self-efficacy in working with LGBTQ students but showed decreased efficacy in teaching about LGBTQ content and helping reduce preservice teacher LGBTQ bias. Participants suggested that time in the curriculum, lack of knowledge about LGBTQ topics/issues, and the lack of institutional support are some of the leading barriers to LGBTQ inclusion in the social studies teacher preparation curriculum.Originality/valueThis is the only work conducted at this scale to examine social studies teacher educators' self-efficacy in LGBTQ-inclusion in methods courses. It has implications for increasing this self-efficacy to help make P-16 social studies education LGBTQ-inclusive.
The study on role of media diversity and political interest on echo chamber effect
Authors: Sakhiya Palak , Rathod Raju 
Published: March 2024

Purpose
Social media has made people better informed but also easier to manipulate. By using literature review and observing social media, the authors found a problem about echo chamber effect. The purpose of this paper is to know how the echo chamber affects the people who consume political news and the role of media diversity in it.

Design/methodology/approach
To conduct this study, the authors used a structured questionnaire on the Qualtrics platform to collect data from 183 participants. The authors collected data using a simple random technique. This study is based on the cross-sectional survey; the data collection period is from October to November 2023. The authors used the SPSS software to analyze the relationships between the variables and test the hypothesis.

Findings
This study found that; echo chamber is not affected by media diversity. Because of increased political interest, people will be less influenced by echo chambers. In addition, demographic factors affect political interest. People use search engines and social media sites instead of political websites when it comes to the consumption of political news online. People like to communicate with individuals who hold conflicting political views.

Originality/value
Researchers have not yet been able to gain a clear understanding of whether users are in an echo chamber or not and how they interact in that environment. Research on this topic is still going on from different perspectives. This study helped to clarify whether or not more media consumption will affect echo chambers. The possibility of being trapped in an echo chamber exists whether we use a single medium or a variety of media. The novelty of this study lies in the use of the echo chamber scale to investigate a thorough understanding of this word through the use of many factors.
Out of sight, but not out of season: Nitrifier distributions and population dynamics in a large oligotrophic lake
Authors: Peoples Logan M. , Seixas Miranda H. , Evans Kate A. , Bilbrey Evan M. , Ranieri John R. , Tappenbeck Tyler H. , Dore John E. , Baumann Adam , Church Matthew J. 
Published: March 2024

AbstractNitrification is an important control on the form and distribution of nitrogen in freshwater ecosystems. However, the seasonality of nitrogen pools and the diversity of organisms catalyzing this process have not been well documented in oligotrophic lakes. Here, we show that nitrogen pools and nitrifying organisms in Flathead Lake are temporally and vertically dynamic, with nitrifiers displaying specific preferences depending on the season. While the ammonia‐oxidizing bacteria (AOB) Nitrosomonadaceae and nitrite‐oxidizing bacteria (NOB) Nitrotoga dominate at depth in the summer, the ammonia‐oxidizing archaea (AOA) Nitrososphaerota and NOB Nitrospirota become abundant in the winter. Given clear seasonality in ammonium, with higher concentrations during the summer, we hypothesize that the succession between these two nitrifying groups may be due to nitrogen affinity, with AOB more competitive when ammonia concentrations are higher and AOA when they are lower. Nitrifiers in Flathead Lake share more than 99% average nucleotide identity with those reported in other North American lakes but are distinct from those in Europe and Asia, indicating a role for geographic isolation as a factor controlling speciation among nitrifiers. Our study shows there are seasonal shifts in nitrogen pools and nitrifying populations, highlighting the dynamic spatial and temporal nature of nitrogen cycling in freshwater ecosystems.
Does the internet bring food prices closer together? Exploring search engine query data in Iran
Authors: Zamani Omid , Bittmann Thomas , Loy Jens‐Peter 
Published: March 2024

AbstractModern communication technologies make information more easily and quickly accessible, leading to more transparent and competitive markets. Based on a theoretical model, this paper provides new empirical evidence on the potential impact of online search intensity on asymmetric cost pass‐through. Prices often move as ‘rockets and feathers’: they rise quickly in response to cost increases and they fall slowly in response to cost reductions. A panel threshold error correction model is applied to weekly producer and retail prices of chicken and mutton in Iran. The results suggest that the volume of online searches is associated with a more complete and less asymmetric cost pass‐through from farmgate to retail prices. Thus, online platforms and search engines have the potential to increase competition by bringing prices closer together and reducing profit margins.