bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced healing of mitomycin C‐treated wounds in rats using inbred adipose tissue‐derived stromal cells within an atelocollagen matrix
Authors: Nambu Masaki , Ishihara Masayuki , Nakamura Shingo , Mizuno Hiroshi , Yanagibayashi Satoshi , Kanatani Yasuhiro , Hattori Hidemi , Takase Bonpei , Ishizuka Takamitsu , Kishimoto Satoko , Amano Yoshiko , Yamamoto Naoto , Azuma Ryuichi , Kiyosawa Tomoharu 
Published: July 2007

ABSTRACTThe aim of this study was to evaluate the potential accelerating effects of an adipose tissue‐derived stromal cells (ATSC)‐containing atelocollagen matrix with silicone membrane (ACMS) for repairing mitomycin C‐treated healing‐impaired wounds. Mitomycin C was applied to full‐thickness skin incisions in this study to create a healing‐impaired wound model in rat. After thoroughly washing out the mitomycin C from the wound, ACMS alone or ATSC‐containing ACMS was applied to the wounds. Histological sections of the wounds were then prepared at indicated time periods after the treatments. These results indicated significantly advanced granulation tissue and capillary formations in the healing‐impaired wounds treated with ATSC‐containing ACMS compared with those treated with ACMS alone. Thus, this study suggested that transplantation of inbred ATSC‐containing ACMS is effective for repairing healing‐impaired wounds.
Delayed wound healing in Mac‐1–deficient mice is associated with normal monocyte recruitment
Authors: Sisco Mark , Chao Jerome D. , Kim Injoong , Mogford Jon E. , Mayadas Tanya N. , Mustoe Thomas A. 
Published: July 2007

ABSTRACTThe Mac‐1 integrin is an important mediator of migration and inflammatory activation of neutrophils and monocytes. However, the role of Mac‐1 in modulating macrophage emigration and activation and its subsequent impact on cutaneous wound healing have not been fully elucidated. To examine the significance of Mac‐1 to murine wound healing, we measured epithelialization and granulation tissue formation in partial‐thickness ear wounds and full‐thickness head wounds, respectively, in Mac‐1–deficient mice. Wounds were histologically analyzed at postwounding days 3, 5, and 7. The gap measured between the leading edges of inward‐migrating granulation tissue was significantly increased in knockout mice compared with control animals at day 5 (3.8±0.3 vs. 2.6±0.5 mm; p<0.001) and day 7 (2.2±0.4 vs. 0.96±0.73 mm; p=0.005). Epithelial gap measurements were also increased in knockout mice vs. wild‐type controls at days 3 (0.62±0.02 vs. 0.54±0.07 mm; p<0.05) and 5 (0.58±0.06 vs. 0.39±0.08 mm; p<0.001). Immunohistochemistry showed equal numbers of macrophages in knockout and control wounds. These findings show that Mac‐1 is required for normal wound healing but that the attenuation in the deposition of granulation tissue and wound epithelialization in Mac‐1 knockout mice is not associated with decreased monocyte migration into the wound.
Assertive community treatment in the Netherlands: a randomized controlled trial
Authors: Sytema S. , Wunderink L. , Bloemers W. , Roorda L. , Wiersma D. 
Published: July 2007

Objective:  Assertive community treatment is rapidly implemented by many European mental health services, but recently the evidence base has been questioned. Positive results of randomized trials in the USA were not replicated in the UK. The question is whether the UK findings are representative for other European countries with modern mental health services.Method:  Open randomized controlled trial of long‐term severely mentally ill patients [Health of the Nation Outcome Scales (HoNOS) total score ≥15], assigned to assertive community treatment (n = 59) or to standard community mental health care (n = 59). Primary outcome: sustained contact; housing stability and admission days. This trial is registered as an International Standard Randomized Clinical Trial, number ISRCTN 11281756.Results:  Assertive community treatment was significantly better in sustaining contact with patients, but not in reducing admission days. No differences in housing stability, psychopathology, social functioning or quality of life were found.Conclusion:  The results are in agreement with UK studies. However, the sustained contact potential of assertive community treatment is important, as too many patients are lost in standard care.
Application of optical coherence tomography in non‐invasive characterization of skin vascular lesions
Authors: Salvini Camilla , Massi Daniela , Cappetti Alessio , Stante Marcello , Cappugi Pietro , Fabbri Paolo , Carli Paolo 
Published: July 2007

Background: Optical coherence tomography (OCT) is a new non‐invasive approach for real‐time in vivo tissue characterization. A promising use of OCT can be the assessment of the architecture of lesions with some degree of inhomogeneities, such as vascular lesions. Knowledge of the size and depth of the vascular structures can be useful for the diagnosis and for choosing the best treatment.Objective: The purpose of this study was to investigate a series of vascular lesions by means of OCT in order to obtain new insights into the non‐invasive, pre‐operative analysis of these lesions.Methods: Seven vascular lesions were included in the study. Histopathological diagnosis showed two haemangiomas and one haemolymphangioma; the remaining four cases were classified as haemangiomas on the basis of their clinical appearance.Results: In all lesions, OCT analysis was able to visualize different areas of the lesion from the horny layer to the dermis showing a clear image of the vascular proliferation. Specifically, oval to roundish signal‐poor areas sharply demarcated by a surrounding signal‐rich layer were observed in good correlation with histopathology.Conclusion: The analysis of vascular lesions by OCT provides a new insight into non‐invasive diagnosis and can be helpful in the selection of the most appropriate treatment.
Changes of skin characteristics during and after local Parafango therapy as used in physiotherapy
Authors: Clijsen Ron , Taeymans Jan , Duquet William , Barel André , Clarys Peter 
Published: July 2007

Background/aims: In physiotherapy, fango (mud) application is a frequently used heat therapy. The main therapeutic effects are due to the elevated temperature of the different tissues with a significant redistribution of blood towards the heated area. This may influence several cardiovascular parameters. There is only limited information on the effect of fango application on skin characteristics. It was the aim of the present study to evaluate the effects of fango application on skin temperature, perfusion of the microcirculation and skin colour. At the same time, cardiovascular parameters such as heart rate, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were recorded.Method: Eighteen healthy subjects (age 23.7±3.8 years) entered the study. The skin characteristics and cardiovascular parameters were measured before, during and after a 21‐min fango application at 44.5°C.Results: Skin temperature and perfusion of the microcirculation increased significantly during fango application: from 35.5±0.4°C to 44.3±1.2°C for skin temperature and from 23.2±8.8 to 197±41 p.u. for the skin microcirculation. These two parameters remained elevated during the fango application and decreased slowly to baseline values within 21 min after fango removal. Skin colour (CIELAB, a* parameter) increased from 11.0±2.5 to 17.9±1.9 when comparing pre‐ with post‐treatment values. At the end of the measuring period, the a* parameter did not return to baseline values (15.8±2.1). Heart rate increased with 8 bpm during the fango therapy and returned to baseline within 3 min after removal of the fango. SBP and DBP varied slightly during the fango application. They returned to baseline values within 21 min after fango removal.Conclusion: The skin parameters indicate a transient temperature effect with an increased perfusion of the microcirculation and a flooding of the superficial capacitance system. The cardiovascular parameters were only slightly influenced and remained in the physiological range. Fango application seems not to be too demanding for the cardiovascular system in healthy subjects.
Cosmetic effectiveness of topically applied hydrolysed keratin peptides and lipids derived from wool
Authors: Barba C. , Méndez S. , Roddick‐Lanzilotta A. , Kelly R. , Parra J. L. , Coderch L. 
Published: July 2007

Background/purpose: Skin moisturisation, elasticity, feel and appearance can all be improved through the topical application of protein hydrolysates. Recent studies suggest that supplementing intercellular lipids of the stratum corneum can enhance the functioning of the skin.Methods: In this study, a hydrolysed keratin peptide (molecular weight <1000 Da) was prepared from wool and tested on skin in two different formulations: an aqueous solution and an internal wool lipids (IWL) liposome suspension. In vivo long‐term studies were performed to evaluate the water barrier function of the skin after topical application of different formulations. During the treatment period, hydration and elasticity were determined. A sorption–desorption test was also performed to assess the hygroscopic properties and water‐holding capacity of the different treated skin sites.Results: Significant differences were found between the control and treated sites, with the treated areas showing an increase in hydration and elasticity as a result of keratin peptide application. Measurements also indicated that the keratin formulations reinforce the skin barrier integrity, improving its water‐holding capacity.Conclusion: A combination of the keratin peptide with the IWL showed beneficial effects, indicating that this combination is suitable for designing new cosmetics products.
Microbial changes in patients with acute periodontal abscess after treatment detected by PadoTest
Authors: Eguchi T , Koshy G , Umeda M , Iwanami T , Suga J , Nomura Y , Kawanami M , Ishikawa I 
Published: July 2007

Aims:  To investigate changes in bacterial counts in subgingival plaque from patients with acute periodontal abscess by IAI‐PadoTest.Materials and methods:  Ninety‐one patients were randomly allocated to either test or control groups. In all the patients, pockets with acute periodontal abscess were irrigated with sterilized physiological saline, and in the test group, 2% minocycline hydrochloride ointment was applied once into the pocket in addition. Subgingival plaque samples were collected by paper point before treatment and 7 days after treatment. The total bacterial count was determined and Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, andTannerella forsythia, were detected using IAI‐PadoTest, a DNA/RNA probe method.Results:  The total bacterial count decreased in both groups, with a significant decrease in the test group. The counts and number of sites positive for P. gingivalis, T. forsythia and T. denticola significantly decreased in the test group after treatment, compared with those in the control group. Pocket depth decreased in the both groups, with a statistically significant decrease in the test group.Conclusion:  Topical treatment with minocycline in pockets with acute periodontal abscess was effective in reducing the bacterial counts as shown by the microbiological investigation using PadoTest 4.5.
Molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis
Authors: Riggio MP , Lennon A , Rolph HJ , Hodge PJ , Donaldson A , Maxwell AJ , Bagg J 
Published: July 2007

Aim:  Compare the microbial profiles on the tongue dorsum in patients with halitosis and control subjects in a UK population using culture‐independent techniques.Materials and methods:  Halitosis patients were screened according to our recently developed recruitment protocol. Scrapings from the tongue dorsum were obtained for 12 control subjects and 20 halitosis patients. Bacteria were identified by PCR amplification, cloning and sequencing of 16S rRNA genes.Results:  The predominant species found in the control samples were Lysobacter‐type species, Streptococcus salivarius, Veillonella dispar, unidentified oral bacterium, Actinomyces odontolyticus, Atopobium parvulum and Veillonella atypica. In the halitosis samples, Lysobacter‐type species, S. salivarius, Prevotella melaninogenica, unidentified oral bacterium, Prevotella veroralis and Prevotella pallens were the most commonly found species. For the control samples, 13–16 (4.7–5.8%) of 276 clones represented uncultured species, whereas in the halitosis samples, this proportion increased to 6.5–9.6% (36–53 of 553 clones). In the control samples, 22 (8.0%) of 276 clones represented potentially novel phylotypes, and in the halitosis samples, this figure was 39 (7.1%) of 553 clones.Conclusions:  The microflora associated with the tongue dorsum is complex in both the control and halitosis groups, but several key species predominate in both groups.
Clinical oral malodor measurement with a portable sulfide monitor
Authors: Ueno M , Shinada K , Yanagisawa T , Mori C , Yokoyama S , Furukawa S , Takehara S , Kawaguchi Y 
Published: July 2007

Objective:  The aim of this study was to evaluate the clinical ability of Breathtron® by comparing it with other malodor measurement procedures: the organoleptic test (OT) and gas chromatography (GC).Subjects and methods:  Patients were 475 patients who visited a fresh breath clinic. Oral malodor was measured with the OT, GC, and Breathtron®. Correlation analysis and two linear regression analyses were conducted to examine the relationship of the Breathtron® values with OT scores and volatile sulfide compound (VSC) concentrations by GC: i.e. the regression of Breathtron® on OT and the regression of Breathtron® on total VSCs by GC. Receiver operating characteristics (ROC) analysis was conducted to investigate the sensitivity and specificity of Breathtron®.Results:  The Breathtron® values were significantly correlated with OT and VSCs by GC. In the regression analysis, predicted Breathtron® values were 199.3 and 520.1 ppb for OT scores 1 and 2, and reasonably close to total VSCs by GC between 550 and 750 ppb. The ROC analysis demonstrated that Breathtron® is a useful and valuable adjunct measurement tool.Conclusions:  Breathtron® is a simple, rapid and reliable appliance for screening oral malodor if an appropriate malodor threshold level is chosen.
Plasma cortisol and norepinephrine in Alzheimer’s disease: opposite relations with recall performance and stage of progression
Authors: Bemelmans Karel J. , Noort Annemarie , de Rijk Roel , Middelkoop Huub A. M. , van Kempen Godfried M. J. , Goekoop Jaap G. 
Published: July 2007

Objective:Alzheimer’s disease (AD) is characterized by effortful retrieval memory impairments, loss of hippocampal neurons and elevated plasma cortisol (CORT) concentrations. The latter could induce further memory decline. AD is also characterized by increased central and peripheral noradrenergic activity. Since noradrenergic function is involved in memory formation, this upregulated function could counteract memory decline. The aim of the present study was to test these hypotheses using plasma norepinephrine (NE) as a noradrenergic parameter, and recall of the prerecency part of neutral valence word lists as a measure of effortful retrieval.Methods:Area under the curve (AUC) of morning, midday and afternoon plasma CORT and plasma NE concentrations was related to two measures of recall performance, ie summated recall scores of the prerecency and recency parts of three word lists, and to the stage of the Clinical Dementia Rating (CDR).Results:Partial correlation between each hormone AUC value and prerecency recall performance, controlling for the effect of the other hormone, showed opposite relations between recall and either plasma CORT or NE. Similar stronger correlations were found with the CDR score.Conclusions:Plasma CORT and NE are oppositely related with effortful retrieval and the stage of progression in AD.
MMPI-2 clusters of alcohol-dependent patients and the relation to Cloninger’s temperament-character inventory
Authors: Egger Jos I. M. , Gringhuis Marian , Breteler Marinus A. , De Mey Hubert R. A. , Wingbermühle Ellen , Derksen Jan J. L. , Hilberink Sander 
Published: July 2007

Objective:Psychometric research in the field of alcohol dependence has concentrated on identifying certain (personality) characteristics (i.e. typologies). This paper is aimed to identify such typologies and studies the relation of the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) and Cloninger’s temperament-character inventory (TCI).Method:To find MMPI-2 scales associated with maximization of group differences between 222 DSM-IV alcohol dependent inpatients and a control group of 222 normal subjects, discriminant analysis was used. In addition, a cluster analysis was performed with these scales, and the MMPI-2 mean scale values of the resulting patient clusters were examined for their TCI-correlates.Results:The discriminant analyses showed several MMPI-2 scales that could clearly distinguish between alcohol-dependent patients and the normal controls. Cluster analysis resulted in semantically different MMPI-2 profiles implying qualitatively different groups of patients. When related to TCI scales, these differences revealed harm avoidance, self-directedness, and persistence, amongst others, as important elements in the description of the clusters.Conclusion:Evidence for the validity of MMPI-2 constructs as well as those of the TCI in the assessment of alcohol-dependent patients was provided.
Ocular prosthesis: a brief review and fabrication of an ocular prosthesis for a geriatric patient
Authors: Patil Sanjayagouda B. , Meshramkar Roseline , Naveen B. H. , Patil N. P. 
Published: July 2007

Objective:  A review of literature for ocular prosthesis is presented along with a case report of a geriatric patient.Review of literature:  The evolution of ocular prosthesis, materials and methods, fabrication techniques for custom and stock eye prosthesis, delivery and after care for the ocular prosthesis are reviewed.Case report:  A 74‐year‐old female patient who had undergone enucleation of her right eye was restored with a stock ocular prosthesis.Conclusion:  The use of stock ocular prosthesis of appropriate contour, size and colour can provide an acceptable aesthetic result.
Influence of bite force and tongue pressure on oro‐pharyngeal residue in the elderly
Authors: Ono Takahiro , Kumakura Isami , Arimoto Mayumi , Hori Kazuhiro , Dong Juan , Iwata Hisayuki , Nokubi Takashi , Tsuga Kazuhiro , Akagawa Yasumasa 
Published: July 2007

Objective:  To investigate the influence of maximal bite force, maximal tongue pressure, number of mastications and swallowing on the oro‐pharyngeal residue in the elderly.Background:  Oro‐pharyngeal residue in the elderly is an indication of dysphagia. Pharyngeal residue is especially critical as it may cause aspiration pneumonia, which is one of the major causes of death in elderly.Materials and methods:  Videofluorographic recordings were performed on 14 elderly volunteers (six males, eight females, age range 65–93 years) without any history or symptoms of dysphagia. The subjects were instructed to consume 9 g of barium containing bread in two manners; free mastication and swallow (FMS: masticate and swallow freely), and limited mastication and swallow (LMS: swallow once after 30 chewing actions). The amount of oral and pharyngeal residue was evaluated using a 4‐point rating scale. Maximal occlusal force was measured by a pressure sensitive sheet, and maximal tongue pressure using a handy probe. Multiple regression analysis was performed to examine the influence of these items on the amount of oral and pharyngeal residue in FMS and LMS.Results:  In FMS, age was found to be a factor which increased oral residue (p = 0.053), and the number of swallowing (p = 0.017) and the state of the prosthesis (p = 0.030) reduced the pharyngeal residue. In LMS, tongue pressure was a factor which reduced oral residue (p = 0.015) and increased pharyngeal residue (p = 0.008).Conclusion:  It is suggested that in the elderly tongue pressure contributed to propulsion of the food bolus from oral cavity into the pharynx, and multiple swallowing contributed to the reduction in the amount of pharyngeal residue.
Distribution of biofilm on internal and external surfaces of upper complete dentures: the effect of hygiene instruction
Authors: Paranhos Helena de Freitas Oliveira , Da Silva Claudia Helena Lovato , Venezian Giovana Cherubini , Macedo Leandro Dorigan , Souza Raphael Freitas de 
Published: July 2007

Objectives:  The purpose of this study was to evaluate biofilm distribution over internal and external surfaces of upper complete dentures. It was also aimed at assessing the effect of oral hygiene instructions before and after home use of a disclosing solution.Materials and methods:  The sample consisted of 29 complete denture wearers whose upper prostheses were evaluated. Surface biofilm was disclosed by means of a 1% neutral red solution and quantified with regard to internal and external surfaces. Oral hygiene information was provided on an individual basis. During the second stage, an amount of disclosing solution was given for domestic use.Results:  Internal and external surfaces presented a similar amount of biofilm, which was concentrated over the area of the posterior teeth area, palatal rugae and the internal vestibular incline of the distobuccal flange. This pattern was maintained during the study. However, overall amounts were reduced following denture hygiene information. The use of disclosing solution by subjects at home resulted in a further reduction.Conclusions:  It was concluded that biofilm deposits were similar over assessed surfaces, regardless of the type of procedure but irregular areas presented greater amounts. Oral health instruction was effective in reducing the biofilm, in particular when associated with home use of a disclosing agent.