bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of thermal stress on early and late passaged mouse lens epithelial cells
Authors: Bagchi M. , Besser D. , Reddy T.R. , Skoff R. , Maisel H. 
Published: April 2007

AbstractCataract is an age related disease of protein aggregation. It has been suggested that aging affects the cells ability to protect protein integrity. The protein integrity, which is essential for cellular homeostasis, is maintained by a complex system of refolding or degradation of damaged proteins. The heat shock proteins (hsps) are the major contributors in the maintenance of protein integrity. The heat shock transcription factor (HSF‐1) is the master regulator of all hsp synthesis in response to stress. This investigation examined the role of HSF‐1 in the regulation of hsp synthesis in early and late passaged αTN‐4 cells. Data collected in this study revealed that the nucleotide sequence of HSF‐1 mRNA obtained from early and late passaged αTN‐4 cells were identical. When early and late passaged cell were exposed to thermal stress, their hsp expression were also similar. HSP‐40 expression was detected after 2 h of heat stress, whereas HSP‐70 and low molecular weight heat shock protein αβ crystallin showed significantly increased synthesis 18 h post heat stress. The late passaged αTN‐4 cells ability to upregulate hsps in response to heat stress could be due to its high replicative activities. The data presented here suggests a relationship between the presence of functional HSF‐1 and sustained proliferative activities of the late passaged αTN‐4 cell. J. Cell. Biochem. 102: 1036–1042, 2007. © 2007 Wiley‐Liss, Inc.
Nanomechanotransduction and Interphase Nuclear Organization influence on genomic control
Authors: Dalby Matthew J. , Gadegaard Nikolaj , Herzyk Pawel , Sutherland Duncan , Agheli Hossein , Wilkinson Chris D.W. , Curtis Adam SG 
Published: April 2007

AbstractThe ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips® were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors. J. Cell. Biochem. 102: 1234–1244, 2007. © 2007 Wiley‐Liss, Inc.
Inhibition of GSK‐3β decreases NF‐κB‐dependent gene expression and impairs the rat liver regeneration
Authors: Chen Huan , Yang Shengsheng , Yang Zhifeng , Ma Li , Jiang Dandan , Mao Jifang , Jiao Binghua , Cai Zailong 
Published: April 2007

AbstractSerine‐threonine protein kinase glycogen synthase kinase (GSK)‐3 is involved in regulation of many cell functions, but its role in regulating liver regeneration is unknown. Here we investigated the effects of GSK‐3β inhibition on liver regeneration after partial hepatectomy in the rat. The potent and selective GSK‐3β inhibitor SB216763 (0.6 mg/kg intravenously) or vehicle (10% dimethyl sulfoxide) was administered 30 min before 70% partial hepatectomy. Liver regeneration was estimated by the cell proliferation, apoptosis, and the related cell signaling and cycling proteins. In 30 min after hepatectomy in the rat, GSK‐3β was found to be translocated to the nucleus, but GSK‐3β inhibitor SB216763 that could phosphorylate residue Ser9 on GSK‐3β did not attenuated the accumulation. Consequently, the inhibition of GSK‐3β decreased the nuclear factor‐κB activity, the NF‐κB‐dependent gene expression, and COX2 expression, but enhanced p21WAF1/Cip1 transcription. Moreover, the injection of SB216763 impaired the proliferation cell nuclear antigen (PCNA) index and increased the apoptosis of liver compared to the vehicle. GSK‐3β plays an important role in rat liver regeneration. We conclude it may partially result from the inhibition of the NF‐κB pathway and enhancement of p21 WAF1/Cip1 expression. J. Cell. Biochem. 102: 1281–1289, 2007. © 2007 Wiley‐Liss, Inc.
Effect of steroid hormones on Bufo arenarum oviduct. Ultrastructural study
Authors: Medina Marcela Fátima , Crespo Claudia Alejandra , Ramos Inés , Cisint Susana Beatriz , Fernández Silvia Nélida 
Published: April 2007

AbstractThe endocrine regulation of the mucosa of the oviductal pars convoluta was analyzed by ultrastructural studies demonstrating that ovariectomy, together with a decrease in ovarian steroids circulating levels, caused a marked regression in this portion of Bufo arenarum oviduct. Twenty‐five days after ovariectomy, a decrease in the depth of the epithelial and glandular layers was observed due to the notable loss of secretory cells, whose number was clearly smaller than in nonovariectomized females. The remaining secretory cells showed involution signs, with few secretory granules in their cytoplasm, little endoplasmic reticulum near poorly developed Golgi complexes and a large amount of lipid droplets. Cells in an advanced autolysis state were found in the lumen. These characteristics evidence a nonfunctional state of the pars convoluta. Treatment with 5α‐dihydrotestosterone (DHT) completely reversed the ovariectomy effect, inducing pars convoluta growths and restoring the characteristics of epithelial and glandular secretory cells in the whole pars convoluta, with micrographs similar to the control. These same effects were observed after treatment with estradiol‐17β (E2), progesterone (P) o E2+P in the glandular layer of the whole pars convoluta, but only in the epithelial layer of the most anterior region of this duct. In the secretory cells of other segments these treatments induced the formation of granules of high electron density and homogeneous aspect. Each steroid had a particular effect on the pars convoluta. Although E2 and DHT induced the development of the organoids involved in the proteins biosynthesis, P and DHT acted as secretagogues. J. Exp. Zool. 307A:312–323, 2007. © 2007 Wiley‐Liss, Inc.
Synthesis of specifically deuterated adenosine A1 antagonist: BG9928
Authors: Conlon Patrick R. , Kiesman William F. 
Published: April 2007

AbstractBG9928, a high affinity adenosine A1 antagonist, is currently in Phase II clinical trials for the treatment of congestive heart failure. A deuterium‐labeled version of the molecule was synthesized and used as a standard for in vivo pharmacokinetic and in vitro metabolism studies. The labeled form of 3‐[4‐(2,6‐dioxo‐1,3‐dipropyl‐2,3,6,7‐tetrahydro‐1H‐purin‐8‐yl)‐bicyclo[2.2.2]oct‐1‐yl]‐propionic acid (BG9928) was obtained in a convergent manner by joining two major building blocks: the specifically labeled heterocycle 5,6‐diamino‐1,3‐dipropyl‐1H‐pyrimidine‐2,4‐dione (4) and the hemiester 4‐(2‐methoxycarbonyl‐ethyl)‐bicyclo[2.2.2]octane‐1‐carboxylic acid (10). Copyright © 2007 John Wiley & Sons, Ltd.
Synthesis of isotopically labelled thiol volatiles and cysteine conjugates for quantification of Sauvignon Blanc wine
Authors: Hebditch Katherine R. , Nicolau Laura , Brimble Margaret A. 
Published: April 2007

AbstractThe thiols 4‐mercapto‐4‐methylpentan‐2‐ol (4MMPOH), 3‐mercaptohexan‐1‐ol (3MH), and 3‐mercaptohexyl acetate (3MHA), which contribute to the aroma profile of Sauvignon Blanc, as well as other varieties of wine, have been synthesized with deuterium labels. The cysteinylated precursors of the thiols, 4‐(4‐methylpentan‐2‐one)‐L‐cysteine (4MMP‐cys) and 4‐(4‐methylpentan‐2‐ol)‐L‐cysteine (4MMPOH‐cys), found in grape must were also synthesized with deuterium labels. These deuterated compounds provide useful internal standards for the quantification of these thiols in wine using LCMS. Copyright © 2007 John Wiley & Sons, Ltd.
Synthesis of isotopically labelled [3‐14C]‐ and [3,3‐2H2]‐5‐fluoro‐1,3‐dihydro‐1‐hydroxy‐2,1‐benzoxaborole (AN2690), a new antifungal agent for the potential treatment of onychomycosis
Authors: Baker Stephen J. , Zhang Yong‐Kang , Akama Tsutomu , Wheeler Conrad , Plattner Jacob J. , Rosser Richard M. , Reid Ronald P. , Nixon Neil S. 
Published: April 2007

Abstract5‐Fluoro‐1,3‐dihydro‐1‐hydroxy‐2,1‐benzoxaborole (AN2690) is a new antifungal agent for the potential treatment of onychomycosis. During the preclinical development phase, it was necessary to synthesize the radioisotope [3‐14C]‐5‐fluoro‐1,3‐dihydro‐1‐hydroxy‐2,1‐benzoxaborole and the deuterium isotope [3,3‐2H2]‐5‐fluoro‐1,3‐dihydro‐1‐hydroxy‐2,1‐benzoxaborole for in vitro studies. We report the synthesis of these two isotopically labelled derivatives. Copyright © 2007 John Wiley & Sons, Ltd.
Old faces, new places: equity theory in cross‐cultural contexts
Authors: Bolino Mark C. , Turnley William H. 
Published: April 2007

AbstractEquity theory proposes that individuals compare ratios of their own outcomes and inputs to the ratios of outcomes and inputs of others. According to this theory, if the ratios are unequal, individuals are motivated to take actions that will restore equity. Although equity theory has received considerable attention from organizational scholars, relatively few studies have investigated the cross‐cultural applicability of the theory. In this conceptual paper, the value‐orientation model of culture is used to identify the ways in which the cultural context may influence how employees conceptualize inputs and outcomes, their selection of referent others, their equity preferences, and their reactions to inequity. The paper also describes potential avenues for future investigations of equity theory in cross‐cultural settings. Finally, some practical implications of this work are discussed as well. Copyright © 2007 John Wiley & Sons, Ltd.
Raman scattering characterization of well‐aligned RuO2 and IrO2 nanocrystals
Authors: Korotcov Alexandru V. , Huang Ying‐Sheng , Tiong Kwong‐Kau , Tsai Dah‐Shyang 
Published: April 2007

AbstractRaman spectroscopy (RS) has been used as a technique for the characterization of well‐aligned IrO2 and RuO2 nanocrystals (NCs) grown on a number of different oriented substrates under various conditions. The IrO2 and RuO2 NCs were grown via metalorganic chemical vapor deposition and reactive magnetron sputtering. The relative intensity of the three strongest Raman‐active modes, namely, the A1g, B2g and Eg modes, depends on the orientation of NCs and has been used to determine the preferred growth direction of the NCs. The redshifts and asymmetric linewidth broadening of the Raman features of IrO2 and RuO2 NCs with respect to that of their bulk counterparts were analyzed by a modified spatial correlation (MSC) model, which includes a factor of stress‐induced shift. The MSC model showed that the effects of stress and nanometric size could be separated in analyzing the observed Raman features. The usefulness of experimental RS together with the MSC model analysis as a structural and residual stress characterization technique for NCs has been demonstrated. Copyright © 2007 John Wiley & Sons, Ltd.
Atomistic origin and temperature dependence of Raman optical redshift in nanostructures: a broken bond rule
Authors: Gu M. X. , Pan L. K. , Tay B. K. , Sun Chang Q. 
Published: April 2007

AbstractConsistent insight is presented into the atomistic origin and temperature (T) dependence of the redshift of Raman optical modes in nanostructures from the perspective of surface bond unsaturation and the bond‐order‐length‐strength (BOLS) correlation [Sun et al., Phys Rev B 72, 134301 (2005)]. It turns out that: (1) the size‐induced redshift of Raman optical modes results from the effect of bond order deficiency of atoms on the surface and its consequences on the force constants of bonds between the under‐coordinated surface atoms; (2) the temperature‐induced redshift arises from thermally induced bond expansion and bond weakening and (3) the magnitude of vibration of atoms in the surface or surrounding defects is always greater than the fully coordinated bulk atoms, while the frequency of such under‐coordinated atoms are relatively lower. It is demonstrated that a combination of the freedoms of solid size and temperature of testing with Raman spectroscopy could lead to information about the vibrational frequency of a monatomic chain and single‐bond energy, which is beyond the scope of the traditional size‐ and temperature‐independent approaches. Copyright © 2007 John Wiley & Sons, Ltd.
Nanopowders and nanostructured oxides: phase transitions and surface reactivity
Authors: Pagnier T. , Boulova M. , Sergent N. , Bouvier P. , Lucazeau G. 
Published: April 2007

AbstractWe report on experimental characterizations of nanocrystalline oxides by Raman spectroscopy, which has proven to be a reference technique when the scale of the coherent domains becomes too small for X‐ray diffraction. Crystallite‐size modification of ZrO2 and WO3 phase diagrams along with surface modes, surface reactions and adsorbed species characterization on WO3 and SnO2 will be reviewed. Copyright © 2007 John Wiley & Sons, Ltd.
Raman bands of double‐wall carbon nanotubes: comparison with single‐ and triple‐wall carbon nanotubes, and influence of annealing and electron irradiation
Authors: Puech P. , Flahaut E. , Bassil A. , Juffmann T. , Beuneu F. , Bacsa W. S. 
Published: April 2007

AbstractWe compare the G and G′2D bands of single‐, double‐ and triple‐wall carbon nanotubes (CNTs). We observe that the band shape is sensitive to the number of walls of the CNTs. For single‐wall carbon nanotubes (SWCNTs), the G band is composed of two distinct contributions G+ and G−, while the G band for double‐wall nanotubes is composed of one band with two main contributions from the inner and the outer tube. The G′2D band can be fitted with one Lorentzian for single‐wall tubes, while two distinct contributions are observed for double‐wall carbon nanotubes (DWCNTs). Considerable variations of the G′2D band are found with similar first order Raman spectra. Annealing influences the D‐ and RBM‐band intensities. Electron irradiation has the effect of decreasing the G‐ and D‐band wavenumbers but does not enhance the D‐band intensity considerably. The down‐shifts of the G‐ and D‐band wavenumbers are correlated and are the same for two excitation wavelengths. This is consistent with the scattering of phonons around the K‐point. Copyright © 2007 John Wiley & Sons, Ltd.
Quantitative estimation of collisional dephasing rate in the X1A1–A1A2 (410) band of formaldehyde with degenerate four wave mixing spectroscopy
Authors: Carpanese M. , De Dominicis L. , Fantoni R. 
Published: April 2007

AbstractThe application of degenerate four wave mixing (DFWM) spectroscopy to the study of collisional dephasing in the 1A2(410) excited band of formaldehyde has been carried out. The method relies on the measurement of DFWM signal dependence on the partial pressures of different buffer gases and in the saturated regime. Experiments were conducted with CO2, N2 and He as colliding partners to H2CO, and collisional dephasing rates γpd of the order of 1010 s−1 atm−1 were estimated. The experimental findings clearly indicate that coherence decay is the dominant mechanism, with respect to rotational energy transfer (RET), in determining the DFWM signal decreasing with pressure. The comparison between the different values of γpd measured and the literature data for γRET has been used to shed light on key factors mostly ruling the competition between decoherence and population relaxation effects. Copyright © 2007 John Wiley & Sons, Ltd.
Strong‐field dressing of vibrational manifolds within ultrafast coherent Raman excitation
Authors: Gräfe Stefanie , Akimov Denis A. , Böhm Belinda , Zheltikov Aleksei M. , Scully Marlan O. , Kiefer Wolfgang , Engel Volker , Siebert Torsten 
Published: April 2007

AbstractThe mechanism of ultrafast, coherent Raman transitions driven at high field strengths is explored in theory and experiment. The study addresses the possibility of maintaining control of a vibrational wavepacket that is guided to a desired final state during the course of a Raman excitation by significantly perturbing the participating molecular states on the time scale of the dynamics under manipulation. Variations in the sequence and amplitude of the pulsed laser fields give direct insight into the nature of the perturbation as well as the population flow within vibrational manifolds of the electronic states involved in the transition. The excitation scheme realized in this work demonstrates control over the dynamics of a molecular system through a non‐adiabatic pathway that utilizes the full manifold of dressed states induced by the laser fields driving the transition rather than isolating the system in a decoupled dressed state common for the adiabatic passage scheme. By preparing highly excited virbrational states in molecular iodine with this scheme, it is shown that a vibrational wavepacket can be steered by controlling the phase with which the character of the field‐free states is introduced into the manifold of dressed states participating in the transition. This scheme seeks to open the possibility of using ultrafast Raman excitation for initiating and observing chemical reactions in the electronic ground state of a molecular system. Copyright © 2007 John Wiley & Sons, Ltd.
Reference database of Raman spectra of biological molecules
Authors: De Gelder Joke , De Gussem Kris , Vandenabeele Peter , Moens Luc 
Published: April 2007

AbstractRaman spectra of biological materials are very complex, because they consist of signals from all molecules present in cells. In order to obtain chemical information from these spectra, it is necessary to know the Raman patterns of the possible components of a cell. In this paper, we present a collection of Raman spectra of biomolecules that can serve as references for the interpretation of Raman spectra of biological materials. We included the most important components present in a cell: (1) DNA and RNA bases (adenine, cytosine, guanine, thymine and uracil), (2) amino acids (glycine, L‐alanine, L‐valine, L‐serine, L‐glutamic acid, L‐arginine, L‐phenylalanine, L‐tyrosine, L‐tryptophan, L‐histidine, L‐proline), (3) fatty acids and fats (lauric acid, myristic acid, palmitic acid, stearic acid, 12‐methyltetradecanoic acid, 13‐methylmyristic acid, 14‐methylpentadecanoic acid, 14‐methylhexadecanoic acid, 15‐methylpalmitic acid, oleic acid, vaccenic acid, glycerol, triolein, trilinolein, trilinolenin), (4) saccharides (β‐D‐glucose, lactose, cellulose, D‐(+)‐dextrose, D‐(+)‐trehalose, amylose, amylopectine, D‐(+)‐mannose, D‐(+)‐fucose, D‐(−)‐arabinose, D‐(+)‐xylose, D‐(−)‐fructose, D‐(+)‐galactosamine, N‐acetyl‐D‐glucosamine, chitin), (5) primary metabolites (citric acid, succinic acid, fumarate, malic acid, pyruvate, phosphoenolpyruvate, coenzyme A, acetyl coenzyme A, acetoacetate, D‐fructose‐6‐phosphate) and (6) others (β‐carotene, ascorbic acid, riboflavin, glutathione). Examples of Raman spectra of bacteria and fungal spores are shown, together with band assignments to the reference products. Copyright © 2007 John Wiley & Sons, Ltd.
NIR‐FT Raman and FT‐IR spectral investigations of the nonlinear optical chromophore p‐bromoacetanilide
Authors: Jothy V. Bena , Vijayakumar T. , Jayakumar V. S. , Udayalekshmi K. , Ramamoorthy K. , Joe I. Hubert 
Published: April 2007

AbstractVibrational spectral analysis of the hydrogen‐bonded nonlinear optical (NLO) material p‐bromo acetanilide (PBA) was carried out using NIR‐FT‐Raman and FT‐IR spectroscopy. Ab initio molecular orbital computations were performed at HF/6‐31G (d) level to derive equilibrium geometry, vibrational wavenumbers, intensities and first hyperpolarizability. The lowering of the imino stretching wavenumbers suggests the existence of strong intermolecular NH···O hydrogen bonding, which was substantiated by the natural bond orbital (NBO) analysis. The vibrational spectra confirm that the charge‐transfer interaction between the NHCOCH3 group and—Br through phenyl ring is responsible for simultaneous strong IR and Raman activation of the ring mode 8a. Vibrational analysis indicates that the lowering of stretching wavenumbers of methyl group due to electronic effects simultaneously caused by induction and hyperconjugation is due to the presence of the oxygen atom. The presence of blue‐shifting H‐bonds of CH stretching wavenumbers, simultaneous activation of carbonyl stretching mode, the strong activity of low‐wavenumber H‐bond stretching vibrations and the role of intramolecular charge transfer in making the molecule NLO active have been analyzed on the basis of the vibrational spectral features. Copyright © 2007 John Wiley & Sons, Ltd.
Effect of phytate and storage conditions on the development of the ‘hard‐to‐cook’ phenomenon in common beans
Authors: Coelho Cileide Maria Medeiros , de Mattos Bellato Cláudia , Santos Julio Cesar Pires , Ortega Edwin Moises Marcos , Tsai Siu Mui 
Published: April 2007

AbstractPostharvest storage of beans (Phaseolus vulgaris L.) can alter the color, texture, flavor and time required for cooking. These alterations have been associated with the ‘hard‐to‐cook’ phenomenon (HTC) and a reduction in the quality of the grains. HTC has been linked to the genotype, environment and/or storage conditions of the grains, but very few studies have shown the interrelationship between these factors. Therefore, the objective of this study was to analyze the effect of two genotypes, Paraiso and Peruano, grown under the same phosphorus levels, on the development of HTC. These genotypes were evaluated for phytate, protein, tannin and phosphorus contents, hydration time and cooking time when stored for 45, 90 and 135 days, at 29 °C and 5 °C at a relative humidity of 75%. HTC was observed in Peruano after 135 days, which correlated with a reduction in the phytate content. Paraiso did not show HTC even though there was a reduction of tannins during the storage period. The lower storage temperature appeared to control HTC for both genotypes. Overall, the content of phytate can be an indicative factor for the cookability of fresh beans when the relationship between genotype and storage conditions has been determined. Copyright © 2007 Society of Chemical Industry