bluuebunny/crossref_metadata_2025_split huggingface dataset:
Atrial secondary tricuspid regurgitation: pathophysiology, definition, diagnosis, and treatment
Authors: Muraru Denisa , Badano Luigi P , Hahn Rebecca T , Lang Roberto M , Delgado Victoria , Wunderlich Nina C , Donal Erwan , Taramasso Maurizio , Duncan Alison , Lurz Philipp , De Potter Tom , Zamorano Gómez José L , Bax Jeroen J , von Bardeleben Ralph Stephan , Enriquez-Sarano Maurice , Maisano Francesco , Praz Fabien , Sitges Marta 
Published: March 2024

Abstract
Atrial secondary tricuspid regurgitation (A-STR) is a distinct phenotype of secondary tricuspid regurgitation with predominant dilation of the right atrium and normal right and left ventricular function. Atrial secondary tricuspid regurgitation occurs most commonly in elderly women with atrial fibrillation and in heart failure with preserved ejection fraction in sinus rhythm. In A-STR, the main mechanism of leaflet malcoaptation is related to the presence of a significant dilation of the tricuspid annulus secondary to right atrial enlargement. In addition, there is an insufficient adaptive growth of tricuspid valve leaflets that become unable to cover the enlarged annular area. As opposed to the ventricular phenotype, in A-STR, the tricuspid valve leaflet tethering is typically trivial. The A-STR phenotype accounts for 10%–15% of clinically relevant tricuspid regurgitation and has better outcomes compared with the more prevalent ventricular phenotype. Recent data suggest that patients with A-STR may benefit from more aggressive rhythm control and timely valve interventions. However, little is mentioned in current guidelines on how to identify, evaluate, and manage these patients due to the lack of consistent evidence and variable definitions of this entity in recent investigations. This interdisciplinary expert opinion document focusing on A-STR is intended to help physicians understand this complex and rapidly evolving topic by reviewing its distinct pathophysiology, diagnosis, and multi-modality imaging characteristics. It first defines A-STR by proposing specific quantitative criteria for defining the atrial phenotype and for discriminating it from the ventricular phenotype, in order to facilitate standardization and consistency in research.
Genomic Resources and Annotations for a Colonial Ascidian, the Light-Bulb Sea Squirt Clavelina lepadiformis
Authors: Daric Vladimir , Lanoizelet Maxence , Mayeur Hélène , Leblond Cécile , Darras Sébastien 
Published: March 2024

Abstract
Ascidian embryos have been studied since the birth of experimental embryology at the end of the 19th century. They represent textbook examples of mosaic development characterized by a fast development with very few cells and invariant cleavage patterns and lineages. Ascidians belong to tunicates, the vertebrate sister group, and their study is essential to shed light on the emergence of vertebrates. Importantly, deciphering developmental gene regulatory networks has been carried out mostly in two of the three ascidian orders, Phlebobranchia and Stolidobranchia. To infer ancestral developmental programs in ascidians, it is thus essential to carry out molecular embryology in the third ascidian order, the Aplousobranchia. Here, we present genomic resources for the colonial aplousobranch Clavelina lepadiformis: a transcriptome produced from various embryonic stages, and an annotated genome. The assembly consists of 184 contigs making a total of 233.6 Mb with a N50 of 8.5 Mb and a L50 of 11. The 32,318 predicted genes capture 96.3% of BUSCO orthologs. We further show that these resources are suitable to study developmental gene expression and regulation in a comparative framework within ascidians. Additionally, they will prove valuable for evolutionary and ecological studies.
Aging, Race, and Health Disparities: Recommendations From the Research Centers Collaborative Network
Authors: Jain Snigdha , Bey Ganga S , Forrester Sarah N , Rahman-Filipiak Annalise , Thompson Gonzalez Nicole , Petrovsky Darina V , Kritchevsky Stephen B , Brinkley Tina E 
Published: March 2024

Abstract
Racial disparities in adverse health outcomes with aging have been well described. Yet, much of the research focuses on racial comparisons, with relatively less attention to the identification of underlying mechanisms. To address these gaps, the Research Centers Collaborative Network held a workshop on aging, race, and health disparities to identify research priorities and inform the investigation, implementation, and dissemination of strategies to mitigate disparities in healthy aging. This article provides a summary of the key recommendations and highlights the need for research that builds a strong evidence base with both clinical and policy implications. Successful execution of these recommendations will require a concerted effort to increase participation of underrepresented groups in research through community engagement and partnerships. In addition, resources to support and promote the training and development of health disparities researchers will be critical in making health equity a shared responsibility for all major stakeholders.
Leveraging citizen science for monitoring urban forageable plants
Authors: Soares Filipi Miranda , Ferreira Pires Luís , Garcia Maria Carolina , Bouzembrak Yamine , Coradin Lidio , Ghilardi-Lopes Natalia Pirani , Silva Rubens Rangel , de Carvalho Aline Martins , Maculan Benildes Coura Moreira dos Santos , Koffler Sheina , Montedo Uiara Bandineli , Drucker Debora Pignatari , Santiago Raquel , Gavai Anand , de Carvalho Maria Clara Peres , Lima Ana Carolina da Silva , Gabriel Hillary Dandara Elias , de França Stephanie Gabriele Mendonça , de Almeida Karoline Reis , dos Santos Bárbara Junqueira , Saraiva Antonio Mauro 
Published: March 2024

Abstract
Urbanization brings forth social challenges in emerging countries such as Brazil, encompassing food scarcity, health deterioration, air pollution, and biodiversity loss. Despite this, urban areas like the city of São Paulo still boast ample green spaces, offering opportunities for nature appreciation and conservation, enhancing city resilience and livability. Citizen science is a collaborative endeavor between professional scientists and nonprofessional scientists in scientific research that may help to understand the dynamics of urban ecosystems. We believe citizen science has the potential to promote human and nature connection in urban areas and provide useful data on urban biodiversity.
Community antibiotic consumption and associated factors in Lusaka district of Zambia: findings and implications for antimicrobial resistance and stewardship
Authors: Ngoma Maty Tsumbu , Sitali Doreen , Mudenda Steward , Mukuma Mercy , Bumbangi Flavien Nsoni , Bunuma Emmanuel , Skjerve Eystein , Muma John Bwalya 
Published: March 2024

Abstract

Introduction
Antimicrobial resistance (AMR) is a global public health crisis. This study assessed the general public’s consumption of antibiotics and associated factors in the Lusaka district of Zambia.

Methods
This cross-sectional study was conducted among 2038 participants between December 2022 and January 2023. Data were analysed using Stata 13.0. Multivariable regression techniques were used to determine the factors that influenced antibiotic consumption.

Results
Of the 2038 participants, 53.4% were female, and 51.5% had attended at least secondary school. Antibiotic use was 99.2%, of which 40.9% were appropriately used. Overall, 79.1% of antibiotics were prescribed in hospitals, while 20.9% were used from leftovers and accessed without prescriptions. This study found that the appropriate use of antibiotics was associated with being female, being aged 35 years and above, attaining secondary school or tertiary education, having a monthly expenditure of 195 USD and above, being aware that antibiotics were not the same as painkillers, and being confident that when someone was hospitalized, they would get well.

Conclusions
This study found that the appropriate use of antibiotics was low, and this is an urgent public health issue requiring community engagement in tackling AMR and adherence to treatment guidelines in healthcare facilities. Additionally, there is a need to implement and strengthen antimicrobial stewardship programmes in healthcare facilities to promote the rational use of antibiotics in Zambia. There is also a need to heighten community awareness campaigns and educational activities on the appropriate use of antibiotics.
A comprehensive approach for evaluating the virucidal performance of domestic laundry detergents under practical conditions
Authors: Konkol Justyna E , Becker Britta , Paulmann Dajana , Steinmann Eike , Todt Daniel , Meister Toni L , Evers Stefan , Weide Mirko , Dotzauer Andreas , Brill Florian H H 
Published: March 2024

Abstract

Aims
We aimed to develop a method to assess the virucidal performance of domestic laundry in a lab-scale washing machine (Rotawash) based on EN 17658.

Methods and results
For method development, virus recovery was investigated after drying on cotton carriers for three test viruses murine norovirus (MNV), modified vaccinia virus Ankara (MVA), and bovine coronavirus (BCoV), followed by washing simulations in flasks and Rotawash. MNV and MVA demonstrated sufficient recovery from carriers after drying and washing (up to 40°C and 60 min). BCoV exhibited lower recovery, indicating less relevance as a test virus. Rotawash efficacy tests conducted with MNV, a resistant, non-enveloped virus, showed limited efficacy of a bleach-free detergent, aligning with results from a domestic washing machine. Rotawash washes achieved higher reductions in infectious virus titers than suspension tests, indicating the role of washing mechanics in virus removal.

Conclusions
This study established a practical method to test the virucidal efficacy of laundry detergents in Rotawash, simulating domestic washing.
Effects of photoperiod and temperature on the developmental duration and diapause in Dolycoris baccarum (Heteroptera: Pentatomidae) from Hohhot, Inner Mongolia, China
Authors: Tian Ruilin , Hou Zhanming , Li Song , Chai Hua 
Published: March 2024

Abstract
The shield bug, Dolycoris baccarum (L.) (Heteroptera: Pentatomidae), is widely distributed across Asia and Europe. At high latitudes, it overwinters, as adult in diapause, which then becomes the insect source for the following year. To fully understand the developmental duration and diapause characteristics of D. baccarum, the effects of photoperiod and temperature were studied in a population from Hohhot, Inner Mongolia, China. The results indicated that the developmental duration was significantly prolonged at temperatures of 20 or 25 °C, with a prolonged light period; however, when the light period was prolonged to 16L:8D and 18L:6D, the developmental duration was shortened significantly. Furthermore, the developmental duration was also shortened significantly with increasing temperature, when the photoperiod was 12L:12D for short days and 16L:8D for long days. All individuals entered diapause under short-day conditions of 10L:14D and 12L:12D at a temperature of 20 °C; however, the diapause rate decreased significantly under 14L:10D and 16L:8D photoperiods, and the diapause rate decreased significantly at a temperature of 25 °C with prolonged photoperiod. Interestingly, when the photoperiod was fixed at 12L:12D, the diapause rates at different temperatures (20, 25, 28, and 30 °C) exceeded 95%; while the effect of temperature on diapauses was nonsignificant under this photoperiod, it was still sensitive to the photoperiod; at a photoperiod of 16L:8D, the effect of temperature on the diapause rate was noticeable, and the diapause rate decreased significantly with increasing temperature.
Therapeutic Singing and Semi-Occluded Vocal Tract Exercises for Individuals with Parkinson’s Disease: A Randomized Controlled Trial of a Single Session Intervention
Authors: Lee Sun Joo , Dvorak Abbey L , Manternach Jeremy N 
Published: March 2024

Abstract
Individuals with Parkinson’s disease (PD) experience speech and voice-related symptoms that diminish communication and quality of life. Semi-occluded vocal tract (SOVT) exercises are targeted interventions that, when combined with the positive psychosocial benefits of therapeutic group singing (TGS), may affect outcomes. The purpose of this study was to explore the effectiveness of SOVT exercises, specifically straw phonation combined with TGS, to improve voice quality and mood for individuals with PD. We used a true experimental pretest–posttest between-subjects design (i.e., randomized controlled trial) facilitated by a board-certified music therapist. All participants (N = 27) were randomly assigned to one of three groups (a) straw phonation combined with TGS (SP + TGS, n = 10), (b) TGS (n = 10), and (c) speaking-only control group (n = 7). Participants completed voice recordings for acoustic measures and the Visual Analogue Mood Scale for mood analysis before and after a 30-min intervention. The results demonstrated significant improvement in voice quality evidenced by decreasing Acoustic Voice Quality Index scores following a single session for both SP + TGS and TGS intervention groups when compared to the control. Happiness scores improved in the experimental groups when compared to control. Although not statistically significant, participants in the experimental groups (SP + TGS, TGS) demonstrated better mean mood scores on happiness, anxiety, and angry when compared to control, indicating a positive psychological response to the singing interventions. Overall, this study indicated the effectiveness of SP + TGS and TGS as promising therapeutic interventions for voice quality and mood in individuals with PD.
(007) Impact of Non-Invasive Oestrus Synchronization on Sexual Behavior in Intact Sprague Dawley Rats
Authors: Seijas G , Comerma Steffensen S G , Gomez Del Val A , Wegener G , Simonsen U 
Published: March 2024

Abstract

Introduction
Female sexual behavior animal translation data do not translate to acute results and neither current synchronization invasive models (ovariectomy+Estrogen “E2”+Progesterone “P4”) guarantee hormonal physiological values.

Objective
Therefore, our study investigated the effect of non-invasive oestrus synchronization on sexual behavior.

Methods
Methods: Twelve week old Sprague Dawley intact rats were synchronized and randomly divided into three groups each of n=8-10. Each group received a dose of prostaglandin F2α “PGF2α” (75, 125 and 250 μg) at days 0 and 3, and 1 mg of progesterone at day four. Vaginal cytology was performed to determine the point of the cycle and the animals underwent an incentive test. To investigate the sexual behavior, the synchronized female rats were paired with trained male rats, and behavior recorded. The videos were quantified by trained operators. Sexual behavior was quantified as paracopulatory (hops, darts, ear wiggling, sniffing from male and from female) or copulatory (male mounting female, thrusting from male, intromission, ejaculation, female for sniffing position, lordosis, orgasm, female mounting on male, female rotation in her axis and female licking itself), E2 and Follicle Stimulating hormone (FSH) in plasma and E2 in vaginal fluid were quantified by immune assay.

Results
Based on the vaginal cytology, the proportion of animals in proestrus (p), estrus (e) or metestrus (m) was calculated with values of 0.5 (p), 0.25 (e) and 0.25 (m) for the group which received 0.75 μg PGF2α; 0.4 (p), and 0.6 (e) for the group which received 0.125 μg PGF2α; 0.2 (p), 0.5 (e), and 0.3 (m) for the group which received 250 μg PGF2α. The numbers of hops was higher in the proestrus compared to the metestrus, and of mounts higher in the estrus compared to the metestrus phase. The sexual encounter profile and E2 levels were unchanged with the different PGF2α doses, but in the high-dose group no hops were observed. The levels of sexual behavior was comparable to previous studies of hormone substituted ovariectomized rats, but E2 and FSH plasma levels were in a physiological range.

Conclusions
In summary, the non-invasive synchronization protocol could substitute the state-of-the-art used ovariectomized+hormone (E2) model. Our findings suggest the largest part of rats were synchronized in the oestrus phase with 125 μg PGF2α. but the 250 μg had also a positive profile.

Disclosure
Any of the authors act as a consultant, employee or shareholder of an industry for: Initiator Pharma A/S.
(009) Erogenous Zones of the Vagina and Vulva: An Anatomic Map-Based Study
Authors: Stelmar J , Zaliznyak M , Isaacson D , Duralde E , Sandhu S , Mallavarapu S , Smith S , Gaither T , Topp K , Garcia M 
Published: March 2024

Abstract

Introduction
Erogenous sensation zones (ESZs) of the body elicit sexual arousal in response to touch. Prior studies have established general maps of external ESZs, but they did not assess internal surfaces or focus on individual structures of the genitalia.

Objective
To develop a topographic map of the ESZs of the vulva and vagina.

Methods
A sample of n=451 sexually active cisgender women (mean age 48.9 ± 15.5 years) were administered an anonymous, online questionnaire via Qualtrics. Respondents selected predefined areas from anatomic illustrations of the vulva and vagina that bring them pleasure when touched during sex. Respondents then rated the importance of each zone to their sexual pleasure on a 10-point Likert scale.

Results
More respondents selected at least one area of the vulva as an ESZ (82%), as compared to the vagina (59.4%). Of the vulva (Figure 1), the clitoris, peri urethra, and vaginal introitus were the three most frequently selected and highest rated structures. The five ESZs of the vagina (Figure 2) revealed a preference for the superficial anterior region of the vaginal canal. The cervix and posterior areas were selected and rated lower than the superficial aspects.

Conclusions
The vulva and vagina were associated with enhanced erogenous sensation not only at (but also in regions immediately peripheral to) the clitoris. These ESZs were also rated as "highly important" to sexual pleasure. Notwithstanding these trends, the wide range of selection-frequency and pleasure-importance ratings emphasizes the subjective and varying experience of sexual arousal between individuals. These ESZ maps may enhance patient education, particularly among individuals who suffer from sexual dysfunction or other factors that lend to diminished sexual arousal.

Disclosure
No.
(010) Assessment of Cannabis Use Before Partnered Sex on Women who Experience Orgasm Difficulty
Authors: Mulvehill S , Tishler J 
Published: March 2024

Abstract

Introduction
Up to 41% of women experience female orgasm disorder/difficulty (FOD), a statistic unchanged for 50 years. Despite this, there is a paucity of validated treatments. Research has suggested cannabis as a treatment for female sexual disorders for more than 50 years. Yet a literature review revealed no studies evaluating cannabis as a treatment specifically for FOD and no studies comparing women with and without FOD. This study is the first to evaluate cannabis as a treatment for FOD specifically in women with and without FOD.

Objective
Evaluate the effect of cannabis use before partnered sex on women with and without FOD.

Methods
This IRB-approved observational study conducted between March 24, 2022, and November 18, 2022, evaluated baseline demographics, sexual behavior, mental health, cannabis use, and the orgasm subscale questions of the Female Sexual Function Index (FSFI), evaluating orgasm frequency, orgasm satisfaction, and orgasm ease, with and without cannabis before partnered sex.

Results
Of 1,037 survey responses, the researchers received 410 valid, completed surveys; failure to meet the study’s criteria required excluding 23 (5.6%) surveys. Among the 387 valid survey participants, the majority of women, 52% (n = 202) reported orgasm difficulty, were between the ages of 25–34 (45%, n = 91), reported their race as white (75%, n = 152), and were married or in a relationship (82%, n = 165). Among respondents reporting orgasm difficulty (n = 202), cannabis use before partnered sex increased orgasm frequency (72.8%, n = 147/202, p &lt; .001), improved orgasm satisfaction (67%, n = 136/202, p &lt; .001) or made orgasm easier (71%, n = 143/202, p &lt; .001). Frequency of cannabis use before partnered sex correlated with increased orgasm frequency for women with FOD (n = 202, p&lt; .001). Orgasm response to cannabis depended on the reasons for use (n = 202, p = .022). Women with FOD reported 24% more mental health issues, 52.6% more PTSD, 29% more depressive disorders, 13% more anxiety disorders, and 22% more prescription drug use than women without FOD. Women with FOD were more likely to report sexual abuse history than women without FOD (38.6%, n=74/202).

Conclusions
Fifty years of sexuality research support use of cannabis for sexual difficulties. This is the first study to look at FOD specifically, demonstrating significant benefit. Randomized controlled studies are needed to evaluate cannabis’ efficacy on FOD sub-types, mental health and physical health conditions and other clinical implications; evaluating dosage, strain, timing, and method of intake. References Laumann et al., 2005 Kontula & Miettinen, 2016 Keiman & Meston, 1997 Dawley et al., 1979 Gorzalka et al., 2010 Klein et al., 2012 Lewis, 1970 Moser et al., 2023.

Disclosure
Any of the authors act as a consultant, employee or shareholder of an industry for: inhaleMD.
(012) Sexual Dysfunction in Men After Failed Hypospadias Repair
Authors: Shatylko T , Gamidov S , Dusmukhamedov R 
Published: March 2024

Abstract

Introduction
Hypospadias is commonly treated in childhood, and future sexual function of such patients is sometimes overlooked. Some published reports reveal excellent sexual function following hypospadias repair at long term, while other authors state high prevalence of sexual dysfunction which depends on original position of urethral meatus. Patients with complications of primary hypospadias repair are one of the most difficult subpopulations to evaluate. This is a unique subset of patients who should be described separately from men who underwent successful hypospadias repair.

Objective
To describe the spectrum of sexual dysfunction in patients with sequelae of failed hypospadias repair and our experience in its management.

Methods
We evaluated 112 patients with a history of failed hypospadias repair, defined as presence of voiding symptoms or cosmetic problems remaining despite previous surgery or caused by it. Patients’ median age was 29.5 years (range: 18–62). There were no intersex cases. Only 9.8% (11/112) underwent single attempt at surgical reconstruction, the median number of attempts was 3.5 (range: 1–9). Patients with IIEF-EF score ≤ 25 were considered to have erectile dysfunction; those with IIEF-EF ≤ 16 underwent penile doppler ultrasound, penile electromyography and endocrine assessment.

Results
64.2% of patients (72/112) were dissatisfied with penile appearance, 40.2% (45/112) had ED, 71.4% (80/112) had ejaculation disorders. Psychogenic ED was diagnosed in 21 patients with preserved non-coital erections and no evidence of organic ED. Median IIEF-EF in ED patients was 20 (range: 8–25). Asthenic ejaculation, delayed ejaculation and anejaculation were present in 63.4% (71/112), 7.1% (8/112) and 3.5% (4/112) patients, respectively. Premature ejaculation was present in 28.6% (32/112) patients, but its association with hypospadias or previous surgery is unlikely. On univariate analysis only the number of previous attempts at surgical repair was a significant predictor of sexual dysfunction following definitive surgery (p = 0.033). Rate of normal sexual function was 37.5%, 23.5% and 9.1% among patients with history of ≤ 3, 4–6 and ≥ 7 procedures, respectively.

Conclusions
Failed hypospadias repair may lead to a wide spectrum of sexual dysfunction, involving sexual desire, erection and ejaculation. Repeated surgery leads to increased risk of sexual dysfunction which may be devastating for patient’s quality of life.

Disclosure
No.