Constraining models of dominance for nonsynonymous mutations in the human genome
Authors: Kyriazis Christopher C. , Lohmueller Kirk E. 
Published: February 2024

AbstractDominance is a fundamental parameter in genetics, determining the dynamics of natural selection on deleterious and beneficial mutations, the patterns of genetic variation in natural populations, and the severity of inbreeding depression in a population. Despite this importance, dominance parameters remain poorly known, particularly in humans or other non-model organisms. A key reason for this lack of information about dominance is that it is extremely challenging to disentangle the selection coefficient (s) of a mutation from its dominance coefficient (h). Here, we explore dominance and selection parameters in humans by fitting models to the site frequency spectrum (SFS) for nonsynonymous mutations. When assuming a single dominance coefficient for all nonsynonymous mutations, we find that numeroushvalues can fit the data, so long ashis greater than ∼0.15. Moreover, we also observe that theoretically-predicted models with a negative relationship betweenhandscan also fit the data well, including models withh=0.05 for strongly deleterious mutations. Finally, we use our estimated dominance and selection parameters to inform simulations revisiting the question of whether the out-of-Africa bottleneck has led to differences in genetic load between African and non-African human populations. These simulations suggest that the relative burden of genetic load in non-African populations depends on the dominance model assumed, with slight increases for more weakly recessive models and slight decreases shown for more strongly recessive models. Moreover, these results also demonstrate that models of partially recessive nonsynonymous mutations can explain the observed severity of inbreeding depression in humans, bridging the gap between molecular population genetics and direct measures of fitness in humans. Our work represents a comprehensive assessment of dominance and deleterious variation in humans, with implications for parameterizing models of deleterious variation in humans and other mammalian species.Author SummaryThe dominance coefficient (h) of a mutation determines its impact on organismal fitness when heterozygous. For instance, fully recessive mutations (h=0) have no effects on fitness when heterozygous whereas additive mutations (h=0.5) have an effect that is intermediate to the two heterozygous mutations. The extent to which deleterious mutations may be recessive, additive, or dominant is a key area of study in evolutionary genetics. However, dominance parameters remain poorly known in humans and most other organisms due to a variety of technical challenges. In this study, we aim to constrain the possible set of dominance and selection parameters for amino acid changing mutations in humans. We find that a wide range of models are possible, including models with a theoretically-predicted relationship betweenhands. We then use a range of plausible selection and dominance models to explore how deleterious variation may have been shaped by the out-of-Africa bottleneck in humans. Our results highlight the subtle influence of dominance on patterns of genetic load in humans and demonstrate that models of partially recessive mutations at amino-acid-changing sites can explain the observed effects of inbreeding on mortality in humans.