bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kekurangan Aplikasi Grammarly dalam Mendeteksi Kesalahan pada Karya Tulis Mahasiswa
Authors: Tambunsaribu Gunawan , Galingging Yusniaty 
Published: March 2024

Kehadiran aplikasi aplikasi hasil kecerdasan buatan (artificial inteligence) di jaman sekarang tidak akan bisa kita hindari. Dalam keseharian kita saat ini, kecerdasan buatan sangat membantu kita dalam menyelesaikan pekerjaan kita. Penelitian ini membahas tentang jenis kekurangan yang dimiliki oleh aplikasi Grammarly sebagai mesin pemeriksa kesalahan tulisan dalam sebuah naskah. Penelitian ini juga memberikan informasi mengenai langkah-langkah atau solusi yang dilakukan manusia, sebagai penulis, untuk mengatasi kekurangan yang dimiliki oleh aplikasi Grammarly. Penelitian menggunakan metode kualitatif. Data dalam penelitian kualitatif berupa kata, frasa, klausa dan kalimat dalam tulisan ilmiah mahasiswa di jurusan Sastra Inggris, Universitas Kristen Indonesia. Analisis data didapatkan oleh peneliti dari hasil observasi peneliti terhadap uji coba penggunaan aplikasi Grammarly. Peneliti melakukan teknik analisis konten. Dalam hal ini peneliti menjelaskan setiap jenis contoh kesalahan penulisan yang dideteksi oleh Grammarly serta penjelasan mengenai langkah-langkah yang dilakukan peneliti dalam memperbaiki kesalahan tersebut. Dari penelitian ini dapat ditemukan bahwa ada beberapa kesalahan penulisan yang bisa dideteksi oleh aplikasi Grammarly yaitu: a. pengetikan huruf (typo); b. kekosongan tanda baca; c. pillihan kata tidak tepat; d. spasi tidak tepat atau berlebihan; e. kalimat bermakna tidak jelas atau ambigu; f. kalimat berstruktur tidak lengkap. Dari temuan ini, penulis menyimpulkan bahwa secanggih teknologi kecerdasan buatan manusia (artificial intelligence), manusia yang akan selalu melakukan penyempurnaan secara manual. Penulis berharap bahwa penelitian dengan topik pembahasan mesin pemeriksa kesalahan penulisan bisa dilanjutkan di kemudian hari oleh peneliti lainnya agar dapat memberikan perbandingan kualitas antara beberapa aplikasi sehingga memberikan banyak pilihan kepada penulis untuk memeriksa kesalahan dalamnaskah tulisannya.
Kata kunci: aplikasi Grammarly, kesalahan penulisan, kecerdasan buatan
CITRA PEREMPUAN DALAM POSTER FILM HOROR INDONESIA: KAJIAN SASTRA FEMINIS
Authors: Isnaini Heri 
Published: March 2024

Poster film digunakan sebagai bagian iklan dan promosi sebuah film. Studio akan mencetak dan memasang poster-poster film agar manarik calon penonton. Adakalanya poster film dibuat bervariasi dengan bentuk visual dan teks yang menarik. Selain itu, foto-foto aktor film juga ditampilkan dalam poster dengan pose yang menarik. Pada genre film horor, poster film yang disajikan seharusnya hanya mengandung simbol-simbol kemistisan, kengerian, ketakutan, dan kegayatan. Akan tetapi, pada poster-poster film horor Indonesia, simbol-simbol tersebut ditambah juga dengan tubuh perempuan sensual yang disajikan sebagai objek yang abjek. Tubuh perempuan sensual pada poster film horor Indonesia menunjukkan citra perempuan yang terabjeksi secara visual. Dengan begitu, konteks penelitian ini menyajikan tanda-tanda visual dalam poster film horor dengan kerangka semiotika. Teori yang digunakan dalam penelitian ini adalah teori kajian sastra feminis yang berusaha menempatkan perempuan pada posisi seharusnya dalam kerangka masyarakat yang patriarki melalui sistem tanda dan simbol. Hasil penelitian menunjukkan bahwa citra perempuan pada poster film horor Indonesia dapat diklasifikasi menjadi (1) citra perempuan yang sensual; dan (2) objek fetis yang metonimi. Keduanya berkelindan pada sistem ekonomi kapitalisme dengan berlindung pada poster film horor yang pada akhirnya hanya sebagai ajang untuk memosisikan perempuan sebagai objek tanda yang abjek dalam konstelasi sistem patriarki.
Kata kunci: Citra perempuan, film, horor, poster, sastra feminis, semiotika
ANALISIS RASIO LIKUIDITAS SEBAGAI ALAT UNTUK MENGUKUR KINERJA KEUANGAN PADA PT UNITED TRACTORS TBK BURSA EFEK INDONESIA
Authors: Pasulu Milka , Gemini Pra 
Published: March 2024

Rumusan masalah dalam penelitian ini adalah bagaimana kinerja keuangan dalam mengukur rasio likuiditas pada PT United Tractors Tbk di Bursa Efek Indonesia. Tujuan penelitian ini adalah untuk mengetahui kinerja keuangan dalam mengukur rasio likuiditas pada PT United Tractors Tbk di Bursa Efek Indonesia.Metode yang digunakan dalam penelitian ini adalah metode kuantitatif yang diperoleh dari dokumentasi resmi data yang telah dipublikasikan pada Bursa Efek Indonesia melalui situs resmi www.idx.co.id. Hasil penelitian ini menunjukkan bahwa, dari hasil perhitungan rata-rata rasio likuiditas pada PT United Tractors Tbk di Bursa Efek Indonesia dilihat dari nilai current ratio menghasilkan angka yang baik, cash ratio menghasilkan angka yang buruk, dan quick ratio menghasilkan angka cukup baik
Description of the viral community in fecal content of common carp (Cyprinus carpio) living in a naturally preserved wetland on Mexican plateau
Authors: Torres-Meza Oscar Abel , Godoy-Lozano Elizabeth Ernestina , Rico-Chávez Oscar , Loza-Rubio Elizabeth , García-Espinosa Gary 
Published: March 2024

IntroductionThe common carp (Cyprinus carpio) is a species that has been used worldwide as a source of protein for humans because of its adaptive capacity to the environment; however, it is also an invasive species. In Mexico, it was introduced to provide an easily accessible source of protein to people in rural areas until its extensive nationwide distribution. The community of viruses in these fish is not completely known. MethodsMassive sequencing, i.e., whole metagenome shotgun (WMS) sequencing, and bioinformatic analysis were used to describe the viral community of common carp (Cyprinus carpio) living in a natural preserved wetland. ResultsThrough sequencer Illumina NextSeq 500/550 we got 484,953,684 sequences from ten samples of fecal content of carp (5 samples to DNA and 5 samples to RNA). The virome comprised 30 viral families (13 DNA families and 17 RNA families), among which viruses related to vertebrates stood out, for example Orthoherpesviridae, Parvoviridae, Astroviridae, Poxviridae, Hantaviridae and Picornaviridae.DiscussionThe most abundant viral taxa corresponded to bacteriophages, most of which are of polyphyletic origin. The results of this work contribute to the knowledge of the different viral taxa found in the common carp in the wild and indicate that these viral taxa may play a role in health and productivity of fish farms.
Site-selective fatty acid chain conjugation of the N-terminus of the recombinant human granulocyte colony-stimulating factor
Authors: Wang Xu-Dong , Su Zhi-Hao , Du Jie , Yu Wei-Jia , Sun Wen-Long 
Published: March 2024

The clinical application of the recombinant human granulocyte colony-stimulating factor (rhG-CSF) is restricted by its short serum half-life. Herein, site-selective modification of the N-terminus of rhG-CSF with PAL-PEG3-Ph-CHO was used to develop a long-acting rhG-CSF. The optimized conditions for rhG-CSF modification with PAL-PEG3-Ph-CHO were: reaction solvent system of 3% (w/v) Tween 20 and 30 mM NaCNBH3 in acetate buffer (20 mmol/L, pH 5.0), molar ratio of PAL-PEG3-Ph-CHO to rhG-CSF of 6:1, temperature of 20°C, and reaction time of 12 h, consequently, achieving a PAL-PEG3-Ph-rhG-CSF product yield of 70.8%. The reaction mixture was purified via preparative liquid chromatography, yielding the single-modified product PAL-PEG3-Ph-rhG-CSF with a HPLC purity exceeding 95%. The molecular weight of PAL-PEG3-Ph-rhG-CSF was 19297 Da by MALDI-TOF-MS, which was consistent with the theoretical value. The circular dichroism analysis revealed no significant change in its secondary structure compared to unmodified rhG-CSF. The PAL-PEG3-Ph-rhG-CSF retained 82.0% of the in vitro biological activity of unmodified rhG-CSF. The pharmacokinetic analyses showed that the serum half-life of PAL-PEG3-Ph-rhG-CSF was 7.404 ± 0.777 h in mice, 4.08 times longer than unmodified rhG-CSF. Additionally, a single subcutaneous dose of PAL-PEG3-Ph-rhG-CSF presented comparable in vivo efficacy to multiple doses of rhG-CSF. This study demonstrated an efficacious strategy for developing long-acting rhG-CSF drug candidates.
Cardiac defects of hypermobile Ehlers-Danlos syndrome and hypermobility spectrum disorders: a retrospective cohort study
Authors: Knight Dacre R. T. , Bruno Katelyn A. , Singh Ayush , Munipalli Bala , Gajarawala Shilpa , Solomon Mahima , Kocsis S. Christian , Darakjian Ashley A. , Jain Angita , Whelan Emily R. , Kotha Archana , Gorelov David J. , Phillips Sabrina D. , Fairweather DeLisa 
Published: March 2024

BackgroundDefective connective tissue structure may cause individuals with hypermobile Ehlers-Danlos syndrome (hEDS) or hypermobility spectrum disorders (HSD) to develop cardiac defects.MethodsWe conducted a retrospective chart review of adult patients treated in the EDS Clinic from November 1, 2019, to June 20, 2022 to identify those with cardiac defects. Echocardiogram data were collected using a data collection service. All EDS Clinic patients were evaluated by a single physician and diagnosed according to the 2017 EDS diagnostic criteria. Patient demographic, family and cardiac history were extracted from self-reported responses from a REDCap clinical intake questionnaire. Patients with at least 1 available echocardiogram (ECHO) were selected for the study (n = 568).ResultsThe prevalence of aortic root dilation in patients with hEDS was 2.7% and for HSD was 0.6%, with larger measurements for males than females and with age. Based on self-reported cardiac history that was verified from the medical record, patients with hEDS with bradycardia (p = 0.034) or brain aneurysm (p = 0.015) had a significantly larger average adult aortic root z-score. In contrast, patients with HSD that self-reported dysautonomia (p = 0.019) had a significantly larger average aortic root z-score. The prevalence of diagnosed mitral valve prolapse in patients with hEDS was 3.5% and HSD was 1.8%. Variants of uncertain significance were identified in 16 of 84 patients that received genetic testing based on family history.ConclusionsThese data reveal a low prevalence of cardiac defects in a large cohort of well-characterized hEDS and HSD patients. Differences in cardiovascular issues were not observed between patients with hEDS vs. HSD; and our findings suggest that cardiac defects in patients with hEDS or HSD are similar to the general population.
Establishment and evaluation of a nomogram for in-hospital new-onset atrial fibrillation after percutaneous coronary intervention for acute myocardial infarction
Authors: Tu Junjie , Ye Ziheng , Cao Yuren , Xu Mingming , Wang Shen 
Published: March 2024

BackgroundNew-onset atrial fibrillation (NOAF) is prognostic in acute myocardial infarction (AMI). The timely identification of high-risk patients is essential for clinicians to improve patient prognosis.MethodsA total of 333 AMI patients were collected who underwent percutaneous coronary intervention (PCI) at Zhejiang Provincial People's Hospital between October 2019 and October 2020. Least absolute shrinkage and selection operator regression (Lasso) and multivariate logistic regression analysis were applied to pick out independent risk factors. Secondly, the variables identified were utilized to establish a predicted model and then internally validated by 10-fold cross-validation. The discrimination, calibration, and clinical usefulness of the prediction model were evaluated using the receiver operating characteristic (ROC) curve, calibration curve, Hosmer-Lemeshow test decision curve analyses, and clinical impact curve.ResultOverall, 47 patients (14.1%) developed NOAF. Four variables, including left atrial dimension, body mass index (BMI), CHA2DS2-VASc score, and prognostic nutritional index, were selected to construct a nomogram. Its area under the curve is 0.829, and internal validation by 10-fold cross-folding indicated a mean area under the curve is 0.818. The model demonstrated good calibration according to the Hosmer-Lemeshow test (P = 0.199) and the calibration curve. It showed satisfactory clinical practicability in the decision curve analyses and clinical impact curve.ConclusionThis study established a simple and efficient nomogram prediction model to assess the risk of NOAF in patients with AMI who underwent PCI. This model could assist clinicians in promptly identifying high-risk patients and making better clinical decisions based on risk stratification.
Genomic analysis in Chilean patients with suspected Rett syndrome: keep a broad differential diagnosis
Authors: Brito Florencia , Lagos Catalina , Cubillos Jessica , Orellana Joan , Gajardo Mallen , Böhme Daniela , Encina Gonzalo , Repetto Gabriela M. 
Published: March 2024

Introduction: Rett syndrome (RTT, MIM #312750) is a rare genetic disorder that leads to developmental regression and severe disability and is caused by pathogenic variants in the MECP2 gene. The diagnosis of RTT is based on clinical features and, depending on resources and access, on molecular confirmation. There is scarce information on molecular diagnosis from patients in Latin America, mostly due to limited availability and coverage of genomic testing. This pilot study aimed to implement genomic testing and characterize clinical and molecular findings in a group of Chilean patients with a clinical diagnosis of RTT.Methods: Twenty-eight patients with suspected RTT underwent characterization of phenotypic manifestations and molecular testing using Clinical Exome SolutionTM CES_V2 by SOPHiA Genetics. Data was analyzed using the commercial bioinformatics platform, SOPHiA DDMTM. A virtual panel of 34 genes, including MECP2 and other genes that are in the differential diagnosis of RTT, was used to prioritize initial analyses, followed by evaluation of the complete exome sequence data.Results: Twelve patients (42.8% of participants) had variants in MECP2, of which 11 (39.2%) were interpreted as pathogenic/likely pathogenic (P/LP), thus confirming the diagnosis of RTT in them. Eight additional patients (28.5%) harbored ten variants in nine other genes. Four of these variants were interpreted as P/LP (14.2%) (GRIN2B, MADD, TRPM3 and ZEB2) resulting in alternative neurodevelopmental diagnoses, and six were considered of uncertain significance. No evident candidate variant was found for eight patients.Discussion: This study allowed to reach a diagnosis in half of the participants. The diagnosis of RTT was confirmed in over a third of them, while others were found to have alternative neurodevelopmental disorders. Further evaluation is needed to identify the cause in those with negative or uncertain results. This information is useful for the patients, families, and clinicians to guide clinical management, even more so since the development of novel therapies for RTT. We also show the feasibility of implementing a step-wide approach to genomic testing in a setting with limited resources.
Profiling antibody epitopes induced by mRNA-1273 vaccination and boosters
Authors: Girard Bethany , Baum-Jones Elisabeth , Best Rebecca L. , Campbell Thomas W. , Coupart Jack , Dangerfield Kyla , Dhal Abhilash , Jhatro Michael , Martinez Brian , Reifert Jack , Shon John , Zhang Minlu , Waitz Rebecca , Chalkias Spyros , Edwards Darin K. , Maglinao Maha , Paris Robert , Pajon Rolando 
Published: March 2024

BackgroundCharacterizing the antibody epitope profiles of messenger RNA (mRNA)-based vaccines against SARS-CoV-2 can aid in elucidating the mechanisms underlying the antibody-mediated immune responses elicited by these vaccines.MethodsThis study investigated the distinct antibody epitopes toward the SARS-CoV-2 spike (S) protein targeted after a two-dose primary series of mRNA-1273 followed by a booster dose of mRNA-1273 or a variant-updated vaccine among serum samples from clinical trial adult participants.ResultsMultiple S-specific epitopes were targeted after primary vaccination; while signal decreased over time, a booster dose after &gt;6 months largely revived waning antibody signals. Epitope identity also changed after booster vaccination in some subjects, with four new S-specific epitopes detected with stronger signals after boosting than with primary vaccination. Notably, the strength of antibody responses after booster vaccination differed by the exact vaccine formulation, with variant-updated mRNA-1273.211 and mRNA-1273.617.2 booster formulations inducing significantly stronger S-specific signals than a mRNA-1273 booster.ConclusionOverall, these results identify key S-specific epitopes targeted by antibodies induced by mRNA-1273 primary and variant-updated booster vaccination.
CDKL3 shapes immunosuppressive tumor microenvironment and initiates autophagy in esophageal cancer
Authors: Bi Yanping , Liu Jie , Qin Songbing , Ji Fuqing , Zhou Chao , Yang Haihua , Zhou Suna 
Published: March 2024

BackgroundCDKL3 has been associated with the prognosis of several tumors. However, the potential role of CDKL3 in immunotherapy and the tumor microenvironment (TME) in esophageal carcinoma (ESCA) remains unclear.MethodsIn this study, Cox regression analysis was used to assess the predictive value of CDKL3 for ESCA outcomes. We systematically correlated CDKL3 with immunological features in the TME. The role of CDKL3 in predicting the efficacy of immunotherapy was also analyzed. Correlation analysis, Cox analysis and LASSO Cox regression were used to construct the CDKL3-related autophagy (CrA) risk score model. The relationship between CDKL3 expression and postoperative pathological complete response (pCR) rate in esophageal squamous cell carcinoma (ESCC) patients undergoing neoadjuvant chemoradiotherapy (nCRT) was evaluated using Immunohistochemical staining (IHC). The relationship between CDKL3 expression and autophagy induction was confirmed by immunofluorescence staining and western blot, and the effect of CDKL3 expression on macrophage polarization was verified by flow cytometry.ResultsHigh expression of CDKL3 was found in ESCA and was associated with poor prognosis in ESCA. Moreover, CDKL3 expression was negatively correlated with tumor-infiltrating immune cells (TIICs), the integrality of the cancer immunity cycles, and anti-tumor signatures, while CDKL3 expression was positively correlated with suppressive TME-related chemokines and receptors, immune hyperprogressive genes, and suppressive immune checkpoint, resulting in immunosuppressive TME formation in ESCA. An analysis of immunotherapy cohorts of the ESCA and pan-cancer showed a better response to immunotherapy in tumor patients with lower CDKL3 levels. The CrA risk score model was constructed and validated to accurately predict the prognosis of ESCA. Notably, the CrA risk score of ESCA patients was significantly positively correlated with M2 macrophages. Furthermore, knockdown CDKL3 in KYSE150 cells could inhibit autophagy induction and M2 macrophage polarization. And, radiation could downregulate CDKL3 expression and autophagy induction, while ESCC patients with high CDKL3 expression had a significantly lower response rate after nCRT than those with low CDKL3 expression.ConclusionCDKL3 may play an important role in anti-tumor immunity by regulating autophagy to promote the formation of immunosuppressive TME, thus playing a critical role in the prognosis of ESCA.
Impact of programmed cell death protein 1 inhibitor therapy on the survival of patients with advanced or recurrent uterine cancers: a meta-analysis
Authors: Liang Keng-Wei , Chen Liang-Jou , Wang Chun-Hao , Ma Kevin Sheng-Kai , Hsia Li-Hsin , Wang Po-Hui 
Published: March 2024

IntroductionNo prior meta-analysis has investigated the impact of programmed cell death protein 1 (PD-1) inhibitor therapy on survival outcomes in patients with advanced or recurrent uterine cancers (including both corpus and cervical cancers).MethodsA comprehensive search of PubMed and Embase databases was conducted, covering the past 10 years (up to August 2023) and encompassing all clinical research related to uterine cancer. Five randomized controlled trials and one cohort study met the inclusion criteria and were included in the meta-analysis. Data on patient demographics, clinical characteristics, treatment regimens, and survival outcomes were extracted. Hazard ratios (HRs) for overall survival (OS) and progression-free survival (PFS), as well as the relative risk of grade 3 or higher adverse events, were pooled using random-effects models.ResultsPatients receiving PD-1 inhibitors had better OS (HR, 0.65, 95% CI, 0.59–0.72; P&lt;.001) and PFS (HR, 0.59, 95% CI, 0.49–0.70; P&lt;.001) than those receiving variable non-PD-1 inhibitor therapies among 3452 uterine cancer patients. The leave-one-out meta-analysis of the HR of OS showed no individual study impact on the estimation of the overall effect size. Subgroup analysis revealed better OS in the PD-1 inhibitors use than the controls in cervical cancer (HR, 0.68, 95% CI, 0.59–0.79), endometrial cancer (HR, 0.62, 95% CI, 0.54-0.72), and pembrolizumab use (HR, 0.66, 95% CI, 0.57–0.75) subgroups. Patients with advanced cervical cancer, who had CPS &gt; 1, receiving PD-1 inhibitors have statistically significant benefits in OS compared to controls (HR, 0.65, 95% CI, 0.53-0.80). The pooled HR for overall survival was 0.71 (95% CI, 0.60-0.82; P&lt;.001) in patients who received PD-1 inhibitors as compared to those who did not receive PD-1 inhibitors in proficient mismatch repair (MMR) endometrial cancer patients. However, in deficient MMR patients, the HR was 0.30 (95% CI, 0.13-0.70). The relative risk of grade 3 or higher adverse events was not higher in the PD-1 inhibitor group (relative risk, 1.12, 95% CI, 0.98–1.27).ConclusionSurvival was significantly better using PD-1 inhibitor therapy than variable non-PD-1 inhibitor chemotherapies among patients with advanced or recurrent uterine cancers.