bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of priming on distinct immunosuppressive mechanisms of mesenchymal stromal cells under translationally relevant conditions
Authors: Herger Nick , Heggli Irina , Mengis Tamara , Devan Jan , Arpesella Leonardo , Brunner Florian , Distler Oliver , Dudli Stefan 
Published: March 2024

Abstract
Background
The multimodal properties of mesenchymal stromal cells (MSCs), particularly their ability to modulate immune responses is of high interest in translational research. Pro-inflammatory, hypoxic, and 3D culture priming are promising and often used strategies to improve the immunosuppressive potency of MSCs, but the underlying mechanisms are not well understood. Therefore, the aims of this study were (i) to compare the effects of pro-inflammatory, hypoxic, and 3D culture priming on the in vitro immunosuppressive potential of MSCs, (ii) to assess if immunosuppressive priming effects are temporally preserved under standard and translationally relevant culture conditions, and (iii) to investigate if the three priming strategies engage the same immunosuppressive mechanisms.

Methods
Functional in vitro T cell suppressive potency measurements were conducted to assess the impact of pro-inflammatory, hypoxic, and 3D culture priming on the immunosuppressive potential of human bone marrow-derived MSCs. Primed MSCs were either cultured under standard cell culture conditions or translationally relevant culture conditions, and their transcriptomic adaptations were monitored over time. Next-generation sequencing was performed to assess if different priming strategies activate distinct immunosuppressive mechanisms.

Results
(i) Pro-inflammatory, hypoxic, and 3D culture priming induced profound transcriptomic changes in MSCs resulting in a significantly enhanced T cell suppressive potential of pro-inflammatory and 3D culture primed MSCs. (ii) Priming effects rapidly faded under standard cell culture conditions but were partially preserved under translationally relevant conditions. Interestingly, continuous 3D culture priming of MSCs maintained the immunosuppressive potency of MSCs. (iii) Next-generation sequencing revealed that priming strategy-specific differentially expressed genes are involved in the T cell suppressive capacity of MSCs, indicating that different priming strategies engage distinct immunosuppressive mechanisms.

Conclusion
Priming can be a useful approach to improve the immunosuppressive potency of MSCs. However, future studies involving primed MSCs should carefully consider the significant impact of translationally relevant conditions on the preservation of priming effects. Continuous 3D culture could act as a functionalized formulation, supporting the administration of MSC spheroids for a sustainably improved immunosuppressive potency.
Non-cytopathic bovine viral diarrhea virus (BVDV) inhibits innate immune responses via induction of mitophagy
Authors: Li Zhijun , Zhang Ying , Zhao Bao , Xue Qinghong , Wang Chunjiang , Wan Siyu , Wang Jingyu , Chen Xiwen , Qi Xuefeng 
Published: March 2024

AbstractBovine viral diarrhea virus (BVDV) belongs to the genus Pestivirus within the family Flaviviridae. Mitophagy plays important roles in virus-host interactions. Here, we provide evidence that non-cytopathic (NCP) BVDV shifts the balance of mitochondrial dynamics toward fission and induces mitophagy to inhibit innate immune responses. Mechanistically, NCP BVDV triggers the translocation of dynamin-related protein (Drp1) to mitochondria and stimulates its phosphorylation at Ser616, leading to mitochondrial fission. In parallel, NCP BVDV-induced complete mitophagy via Parkin-dependent pathway contributes to eliminating damaged mitochondria to inhibit MAVS- and mtDNA-cGAS-mediated innate immunity responses, mtROS-mediated inflammatory responses and apoptosis initiation. Importantly, we demonstrate that the LIR motif of ERNS is essential for mitophagy induction. In conclusion, this study is the first to show that NCP BVDV-induced mitophagy plays a central role in promoting cell survival and inhibiting innate immune responses in vitro.
Pulmonary function testing in preoperative high-risk patients
Authors: Eimer Christine , Urbaniak Natalia , Dempfle Astrid , Becher Tobias , Schädler Dirk , Weiler Norbert , Frerichs Inéz 
Published: March 2024

Abstract
Background
Postoperative respiratory failure is the most frequent complication in postsurgical patients. The purpose of this study is to assess whether pulmonary function testing in high-risk patients during preoperative assessment detects previously unknown respiratory impairments which may influence patient outcomes.

Methods
A targeted patient screening by spirometry and the measurement of the diffusing capacity of the lung for carbon monoxide (DLCO) was implemented in the anesthesia department of a tertiary university hospital. Patients of all surgical disciplines who were at least 75 years old or exhibited reduced exercise tolerance with the metabolic equivalent of task less than four (MET < 4) were examined. Clinical characteristics, history of lung diseases, and smoking status were also recorded. The statistical analysis entailed t-tests, one-way ANOVA, and multiple linear regression with backward elimination for group comparisons.

Results
Among 256 included patients, 230 fulfilled the test quality criteria. Eighty-one (35.2%) patients presented obstructive ventilatory disorders, out of which 65 were previously unknown. 38 of the newly diagnosed obstructive disorders were mild, 18 moderate, and 9 severe. One hundred forty-five DLCO measurements revealed 40 (27.6%) previously unknown gas exchange impairments; 21 were mild, 17 moderate, and 2 severe. The pulmonary function parameters of forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), and DLCO were significantly lower than the international reference values of a healthy population. Patients with a lower ASA class and no history of smoking exhibited higher FVC, FEV1, and DLCO values. Reduced exercise tolerance with MET < 4 was strongly associated with lower spirometry values.

Conclusions
Our screening program detected a relevant number of patients with previously unknown obstructive ventilatory disorders and impaired pulmonary gas exchange. This newly discovered sickness is associated with low metabolic equivalents and may influence perioperative outcomes. Whether optimized management of patients with previously unknown impaired lung function leads to a better outcome should be evaluated in multicenter studies.

Trial registration
German Registry of Clinical Studies (DRKS00029337), registered on: June 22nd, 2022.
Mom, dad, put down your phone and talk to me: how parental phubbing influences problematic internet use among adolescents
Authors: Liu Saifang , Wu Peiqian , Han Xiaoxi , Wang Mengyun , Kan Yuecui , Qin Kuiyuan , Lan Jijun 
Published: March 2024

Abstract
Background
The positive association of parental phubbing with internalising and externalising problems among adolescents has gained academic traction. To date, limited research has investigated the association of parental phubbing and adolescents’ Problematic Internet Use (PIU). Furthermore, the mechanism underlying this association is largely unknown. These gaps limit our understanding of family-related issues affecting PIU among adolescents. The present study explores whether there is a relation between parental phubbing and PIU and investigates the mechanisms underlying this relation among adolescents.

Methods
The participants were 495 junior high schoolers aged 11–15 years. Participants completed questionnaires on their experiences with PIU, parental phubbing, parent–child relationships, and basic psychological needs satisfaction.

Results
The results showed a direct and indirect positive association between parental phubbing and PIU. Furthermore, parental phubbing indirectly influenced PIU and was mediated by the parent–child relationship and basic psychological needs satisfaction, respectively. Moreover, the parent–child relationship and basic psychological needs satisfaction were sequentially mediated.

Conclusions
Our study highlights the crucial role of parents in the development of adolescent PIU and provides theoretical and practical guidelines for PIU prevention and intervention.
Novel Microemulsion Containing Benzocaine and Fusidic Acid Simultaneously: Formulation, Characterization, and In Vitro Evaluation for Wound Healing
Authors: Arpa Muhammet Davut , Çağlar Emre Şefik , Güreşçi Dilara , Sipahi Hande , Üstündağ Okur Neslihan 
Published: March 2024

AbstractModern drug carrier technologies, such as microemulsions with small droplet sizes and high surface areas, improve the ability of low water solubility active ingredients to permeate and localize. The goal of this study was to create microemulsion formulations for wound healing that contained both fusidic acid (FA), an antibacterial agent, and benzocaine (BNZ), a local anesthetic. Studies on characterization were carried out, including viscosity, droplet size, and zeta potential. The drug-loaded microemulsion had a stable structure with –3.014 ± 1.265 mV of zeta potential and 19.388 ± 0.480 nm of droplet size. In both in vitro release and ex vivo permeability studies, the microemulsion was compared with Fucidin cream and oily BNZ solution. According to the drug release studies, BNZ release from the microemulsion and the BNZ solution showed a similar profile (p > 0.05), while FA release from the microemulsion had a higher drug release compared to Fucidin cream (p < 0.001). The microemulsion presented lower drug permeation (p > 0.05) for both active ingredients, on the other hand, provided higher drug accumulation compared to the control preparations. Moreover, according to the results of in vitro wound healing activity, the microemulsion indicated a dose-dependent wound healing potential with the highest wound healing activity at the highest concentrations. To the best of our knowledge, this developed BNZ- and FA-loaded microemulsion would be a promising candidate to create new opportunities for wound healing thanks to present the active ingredients, which have low water solubility, in a single formulation and achieved higher accumulation than control preparations.
Graphical Abstract
Perspectives on polarity – exploring biological asymmetry across scales
Authors: Glazenburg Marieke Margaretha , Hettema Nynke Marije , Laan Liedewij , Remy Ophélie , Laloux Géraldine , Brunet Thibaut , Chen Xin , Tee Yee Han , Wen Wenyu , Rizvi Mohd Suhail , Jolly Mohit Kumar , Riddell Meghan 
Published: March 2024

ABSTRACT
In this Perspective, Journal of Cell Science invited researchers working on cell and tissue polarity to share their thoughts on unique, emerging or open questions relating to their field. The goal of this article is to feature ‘voices’ from scientists around the world and at various career stages, to bring attention to innovative and thought-provoking topics of interest to the cell biology community. These voices discuss intriguing questions that consider polarity across scales, evolution, development and disease. What can yeast and protists tell us about the evolution of cell and tissue polarity in animals? How are cell fate and development influenced by emerging dynamics in cell polarity? What can we learn from atypical and extreme polarity systems? How can we arrive at a more unified biophysical understanding of polarity? Taken together, these pieces demonstrate the broad relevance of the fascinating phenomenon of cell polarization to diverse fundamental biological questions.
Ocena możliwości wykorzystania punktów widokowych projektowanego Geoparku Małopolski Przełom Wisły w edukacji geomorfologicznej i geoturystyce
Authors: Gajek Grzegorz , Zgłobicki Wojciech 
Published: March 2024

Efektywne działania na rzecz rozwoju geoedukacji i geoturystyki powinny w pierwszej kolejności opierać się o geostanowiska, które mogą budzić zainteresowanie turystów ze względu na swoje walory estetyczne. Warunek taki spełniają punkty widokowe – duża liczba odwiedzających daje szansę na wykorzystanie ich potencjału naukowego. Celami pracy były: a) analiza zasięgu widoczności dla 26 punktów widokowych położonych w obrębie projektowanego Geoparku Małopolski Przełom Wisły, w odniesieniu do występujących tu krajobrazów i form ukształtowania terenu oraz b) ocena ich walorów geoturystycznych w oparciu o autorską metodę. Większość punktów widokowych cechujących się wysokimi walorami naukowo-edukacyjnymi ma również wysokie walory turystyczno-funkcjonalne, co daje dobre podstawy do ich wykorzystania w geoturystyce i geoedukacji. Wskazano punkty widokowe, które mogą stanowić podstawę opracowania szlaku geoturystycznego, prezentującego kluczowe elementy ukształtowania terenu obszaru.
Open-Format Prescription Maps for Variable Rate Spraying in Orchard Farming
Authors: Rovira-Más Francisco , Saiz-Rubio Verónica , Cuenca Andrés , Ortiz Coral , Teruel Montano Pérez , Ortí Enrique 
Published: March 2024

HighlightsStraightforward procedure to code prescription maps independent of manufacturers.Methodology suitable for orchard crops and vineyards including pressures up to 10 bar and flowrates up to 100 L/min.Algorithm validated for both trellis-based continuous rows and traditional orchard rows of discrete trees.Method scalable to other agricultural operations, such as fertilizing or data registration for auditing purposes.Abstract. The implementation of crop protection solutions contributing to significant reductions in the use and risk of chemical pesticides has motivated the interest of site-specific systems, such as variable rate technologies based on user-set prescriptions. However, the practical implementation of variable rates has been dissuasive for many growers who are not familiar with digital solutions, as proprietary firmware is often difficult to grasp. This paper presents a method for formatting prescription maps that is independent of manufacturers, can be easily and freely implemented by industry and can be adopted by growers not familiar with advanced software tools. The methodology was successfully demonstrated for perennial specialty crops, in particular for trellised vineyards in vertical shoot position and for traditional olive groves. Results showed that the variations within user prescription maps were translated to the fields, but flow errors appeared due to the challenge of measuring flow and pressure in the highly pulsating actuation of pulse-width-modulated (PWM) valves. Such errors diminished with the use of low-volume rates and the optimal selection of the nozzles when adjusted to the real pressure measured in the field. The use cases validated in the field showed the consistency of the method for highly populated prescription maps of 1,221 points/ha accessible to advanced farmers, as well as for modest maps of 80 points/ha within reach of the majority of growers. Keywords: Automated map building, Local tangent plane coordinates, Orchard crop operations, Precision farming, Prescription maps, User-centered approach, Variable rate spraying.
Research on the adsorption of Co2+ ions using halloysite clay and the ability to recover them by electrodeposition method
Authors: Thi Duyen Le , Thao Le Thi Phuong , Bac Bui Hoang 
Published: March 2024

Abstract
In environmental research, along with discovering methods for adsorbing heavy metals, it is essential to comprehend the processes of desorption and recovery of these heavy metals from adsorbent materials and their reuse. In this study, halloysite (HAL) clay, obtained from the Thach Khoan, Vietnam, was utilized for the removal of Co2+ ions from an aqueous solution, and the influence of different factors on the adsorption properties of Co2+ was investigated. Optimal conditions determined were 0.8 g HAL mass per 50 mL of solution, initial Co2+ concentration of 40 mg·L−1, contact time of 80 min, pH0 of 6.09, and room temperature of 30°C. Under these conditions, the adsorption efficiency and capacity obtained were 76.358 ± 0.981% and 1.909 ± 0.025 mg·g−1, respectively. The adsorption process followed the Langmuir adsorption isotherms, with a maximum monolayer adsorption capacity of 3.10206 ± 0.13551 mg·g−1, and exhibited a pseudo-second-order kinetic model. Desorption experiments were conducted using the electrochemical method with a deep eutectic solvent based on choline chloride and urea (reline). The results demonstrated that 94.11% of the Co metal could be recovered through electrodeposition after 5 h, using an applied current of 7.5 mA at 60°C. The HAL material was successfully regenerated following the desorption process.
Observation of tunable accidental bound state in the continuum in silicon nanodisk array
Authors: Han Yingying , Xiong Lei , Shi Jianping , Li Guangyuan 
Published: March 2024

Abstract
We experimentally demonstrate the tuning of accidental bound states in the continuum (A-BICs) in silicon nanodisk arrays. The A-BIC emerges of the destructive interference of multipoles, which are the dominating out-of-plane electric dipole and in-plane magnetic dipole, and weak electric quadrupole and magnetic quadrupole. We further show that the spectral and angular position of the A-BIC can be conveniently tuned by varying the nanodisk size or the lattice period. Remarkably, the angular position can be tuned even to 0°, suggesting an interesting transition of the A-BIC from an off-Γ-BIC to an at-Γ-BIC. Our work provides a new strategy for light trapping with high quality factors, and the obtained tunable A-BICs can find potential applications in low-threshold lasing, enhanced nonlinear optics, and optical sensing.
Structural-color meta-nanoprinting embedding multi-domain spatial light field information
Authors: Liang Congling , Wang Jiahao , Huang Tian , Dai Qi , Li Zile , Yu Shaohua , Li Gongfa , Zheng Guoxing 
Published: March 2024

Abstract
Recently, multifunctional metasurface has showcased its powerful functionality to integrate nanoprinting and holography, and display ultracompact meta-images in near- and far-field simultaneously. Herein, we propose a tri-channel metasurface which can further extend the meta-imaging ranges, with three independent images located at the interface, Fresnel and Fourier domains, respectively. Specifically, a structural-color nanoprinting image is decoded right at the interface of the metasurface, enabled by varying the dimensions of nanostructures; a Fresnel holographic image and another Fourier holographic image are present at the Fresnel and Fourier (far-field) domains, respectively, enabled by geometric phase. The spectral and phase manipulation capabilities of nanostructures have been maximized, and the spatial multiplexing capabilities for diffraction in metasurfaces have also been fully exploited. By leveraging the design freedom enabled through the tuning of the geometric size and orientation of nanostructures, as well as optimizing the diffraction spatial light wave transformation, the encoding of multiple images on the single-celled metasurface is achieved. More interestingly, due to the spatial separation of images across different channels, crosstalk is virtually eliminated, effectively enhancing imaging quality. The proposed metasurface offers several advantages, including a compact design, easiness of fabrication, minimal crosstalk, and high storage density. Consequently, it holds promising applications in image display, data storage, information encryption, anti-counterfeiting, and various other fields.
Numerical solution of two-dimensional fractional differential equations using Laplace transform with residual power series method
Authors: Pant Rajendra , Arora Geeta , Singh Brajesh Kumar , Emadifar Homan 
Published: March 2024

Abstract
One of the efficient and reliable methods for resolving fractional order linear as well as non-linear differential equations is the Laplace transform with residual power series method. This approach is used in the current research to obtain the numerical solutions of the two-dimensional fractional differential equations, namely, the temporal fractional order diffusion equation and the fractional biological population equation. The unknown coefficients of the series solutions to these equations are determined using the proposed approach. The difference between exact and analytical-numerical solutions is presented for these equations in the form of errors. The advantage of the suggested method over alternative approaches is that it requires less computation to solve these two-dimensional differential equations of time-fractional order.
Probabilistic analysis of solid oxide fuel-cell integrated with gas turbine
Authors: Gorla Rama Subba Reddy 
Published: March 2024

Abstract
Solid Oxide Fuel Cell (SOFC) integrated with a Gas Turbine (GT) is a highly efficient way to convert chemical energy of hydro carbon fuel into electrical energy. SOFC-GT is a high temperature, high pressure system and features a fuel flexibility, which makes it easier to retrofit into the existing power plants. A lot of research had already taken place to make SOFC-GT more fuel efficient and cost effective as well. SOFC-GT is a complex thermodynamic electro-chemical system that has numerous input variables that affect the overall efficiency of the system. In order to further develop the SOFC-GT technology and future research, clear understanding is required to analyze relationship between the input and output parameters. One way to analyze the input/output relationship over the range of parameter variations is to conduct a probabilistic sensitivity analysis. A probabilistic sensitivity analysis of 19 input variables has been conducted to understand how each variable would affect the net power output and overall efficiency of a SOFC-GT system.
Wunderlich Syndrome Managed with Angiomyolipoma Embolization – Renal Artery Anatomic Variant Augmenting Safe and Nephron-Sparing Intervention
Authors: Tripathy Tara Prasad , Alagappan Alamelu , Patel Ranjan Kumar , Behera Srikant Kumar , Panda Sandip Kumar , Naik Suprava 
Published: March 2024

Background: Wunderlich syndrome is an uncommon entity characterized by spontaneous, nontraumatic renal bleeding into the subcapsular and perirenal regions. The most frequent benign tumor, angiomyolipoma, is the most common cause of Wunderlich syndrome.Case presentation: We report a case of Wunderlich syndrome in angiomyolipoma. Intratumoral pseudoaneurysm arising from feeders of an accessory renal artery supplying the lower pole of the kidney was selectively embolized. Rarely does a sporadic renal angiomyolipoma develop a giant pseudoaneurysm.Conclusion: Transarterial embolization is imperative to control the bleeding or as a preventative measure to reduce the risk of intralesional pseudoaneurysm rupture. When vascular interventional facilities are unavailable, surgery may be necessary.