bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous identification and enantioseparation of ofloxacin and duloxetine without the single standard and computational calculation of their inclusion complexes
Authors: Chen Lixia , Li Xiangjun 
Published: March 2024

AbstractGiven the markedly different pharmacological activities between enantiomeric isomers, it is crucial to encourage the stereoselective determination of chiral drugs in the biological and pharmaceutical fields, and the combination of drugs makes this analysis more complicated and challenging. Herein, a capillary electrophoresis (CE) method for the enantioseparation of ofloxacin and duloxetine was established, enabling the simultaneous identification of four isomers in nonracemic mixtures with enantiomeric excess (ee%) values exceeding 5%. This was achieved through the integration of theoretical simulation and electron circular dichroism (ECD), all without reliance on individual standards. Molecular modeling explained and verified the migration time differences of these isomers in electrophoretic separation. Moreover, the correlation coefficients (R2) between the enantiomeric peak area differentials and ee% were both above 0.99. Recovery rates were quantified using bovine serum as the matrix, with results ranging from 93.32% to 101.03% (RSD = 0.030) and 92.69% to 100.52% (RSD = 0.028) for these two chiral drugs at an ee value of 23.1%, respectively.
Beyond Electrons: Correlation and Self‐Energy in Multicomponent Density Functional Theory
Authors: Holzer Christof , Franzke Yannick J. 
Published: March 2024

AbstractPost‐Kohn‐Sham methods are used to evaluate the ground‐state correlation energy and the orbital self‐energy of systems consisting of multiple flavors of different fermions. Starting from multicomponent density functional theory, suitable ways to arrive at the corresponding multicomponent random‐phase approximation and the multicomponent Green's function
approximation, including relativistic effects, are outlined. Given the importance of both of this methods in the development of modern Kohn–Sham density functional approximations, this work will provide a foundation to design advanced multicomponent density functional approximations. Additionally, the
quasiparticle energies are needed to study light‐matter interactions with the Bethe‐Salpeter equation.
Unremitting pro‐inflammatory T‐cell phenotypes, and macrophage activity, following paediatric burn injury
Authors: Langley Donna , Zimmermann Kate , Krenske Emma , Stefanutti Giorgio , Kimble Roy M , Holland Andrew JA , Fear Mark W , Wood Fiona M , Kenna Tony , Cuttle Leila 
Published: March 2024

AbstractObjectivesThe aim of this study was to characterise the dynamic immune profile of paediatric burn patients for up to 18 months post‐burn.MethodsFlow cytometry was used to measure 25 cell markers, chemokines and cytokines which reflected both pro‐inflammatory and anti‐inflammatory immune profiles. Peripheral blood mononuclear cells from 6 paediatric burn patients who had returned for repeated burn and scar treatments for > 4 timepoints within 12 months post‐burn were compared to four age‐matched healthy controls.ResultsWhile overall proportions of T cells, NK cells and macrophages remained relatively constant, over time percentages of these immune cells differentiated into effector and proinflammatory cell phenotypes including Th17 and activated γδ T cells. Circulating proportions of γδ T cells increased their expression of pro‐inflammatory mediators throughout the burn recovery, with a 3–6 fold increase of IL‐17 at 1–3 weeks, and NFκβ 9–18 months post‐burn. T‐regulatory cell plasticity was also observed, and Treg phenotype proportions changed from systemically reduced skin‐homing T‐regs (CCR4+) and increased inflammatory (CCR6+) at 1‐month post‐burn, to double‐positive cell types (CCR4+CCR6+) elevated in circulation for 18 months post‐burn. Furthermore, Tregs were observed to proportionally express less IL‐10 but increased TNF‐α over 18 months.ConclusionOverall, these results indicate the circulating percentages of immune cells do not increase or decrease over time post‐burn, instead they become highly specialised, inflammatory and skin‐homing. In this patient population, these changes persisted for at least 18 months post‐burn, this ‘immune distraction’ may limit the ability of immune cells to prioritise other threats post‐burn, such as respiratory infections.
Endometrial cytological findings for a mesonephric‐like endometrial adenocarcinoma: A case report
Authors: Omine Hirotaka , Ishida Kazuyuki , Sasaki Natsuki , Kato Hikaru , Nagai Tamiko , Ishikawa Mihoko , Takaoka Mina , Noda Shuhei , Matsuda Hadzki , Mitsuhashi Akira 
Published: March 2024

AbstractA mesonephric‐like endometrial adenocarcinoma (ML‐EAC) is very rare and has a worse prognosis than other endometrial carcinomas. We describe an ML‐EAC and report our endometrial cytological findings. A 76‐year‐old woman presented with irregular genital bleeding and a uterine mass. Endometrial cytology revealed atypical cylindrical or spindle‐shaped cells in the form of small aggregates or solitary cells. The cell aggregates exhibited irregularly stacked papillary structures, small glandular structures, and fenestrated structures. The atypical cells had a nucleus with fine‐granular chromatin and a granular cytoplasm, and nuclear grooves and intranuclear pseudo‐inclusions were present. Hyaline globules were observed in the glandular lumens and in the background. The presumptive histological type was an adenocarcinoma, but the cytological features were different from those of an endometrioid carcinoma. A histological examination of the endometrial biopsy revealed an adenocarcinoma, and a simple hysterectomy was performed. A grayish‐white elevated mass measuring 90 mm × 70 mm × 40 mm was observed on the uterine corpus in the hysterectomy specimen. Histologically, the tumor proliferated as complex tubular structures containing eosinophilic colloid‐like materials and trabecular structures. The tumor cells were diffuse and positive for GATA‐3 and partially positive for thyroid transcription factor‐1. Estrogen and progesterone receptors were negative. An ML‐EAC was diagnosed. The tumor was invasive and extended beyond one‐half of the muscle layer with a high degree of vascular invasion. In conclusion, we need to focus on the various shapes of the cell aggregate, nuclear grooves, and intranuclear pseudo‐inclusions of tumor cells to distinguish an ML‐EAC from other endometrial carcinomas in endometrial cytology.
Consumer insights into the at‐home liking of commercial beers: Integrating nonvolatile and volatile flavor chemometrics
Authors: Ramos‐Parra Perla A. , De Anda‐Lobo Irma C. , Viejo Claudia Gonzalez , Villarreal‐Lara Raúl , Clorio‐Carillo Jorge Abraham , Marín‐Obispo Luis Martín , Obispo‐Fortunato Diana Jessica , Escobedo‐Avellaneda Zamantha , Fuentes Sigfredo , Pérez‐Carillo Esther , Hernandez‐Brenes Carmen 
Published: March 2024

AbstractConsumer acceptability of beers is influenced by product formulation and processing conditions, which impart unique sensory profiles. This study used multivariate techniques to evaluate at‐home consumer sensory acceptability of six commercial beers considering their style, fermentation type, and chemical composition. Samples included top‐fermented beers (American India Pale Ale and Stout) and bottom‐fermented beers (Pilsner, zero‐alcohol Pilsner, Vienna Lager, and Munich Dunkel). Beer consumers (n = 50) conducted sensory hedonic, check‐all‐that‐apply (CATA) and just‐about‐right (JAR) tests. Chemometric variables included iso‐alpha‐acids, hordenine, and volatile aromatic compounds, quantified by chromatographic methods, whereas bitterness units (IBU) were determined spectrophotometrically. Lager beers had higher acceptability than top‐fermented beer (p < .05) for all attributes. Light‐colored beers and medium‐height foams had the highest liking scores for visual sensory attributes. Higher concentrations of bitter‐tasting molecules, hordenine, and acidity decreased the liking scores of top‐fermented (Ale) beers, as a sensory penalty analysis suggested. In contrast, the most favored beers (Pilsners and Munich Dunkel) contained higher fusel alcohol esters linked to fruity aromatic notes. Although a low conversion rate of fatty acids into fruity esters was noted in nonalcoholic Pilsner, its overall liking score was not statistically different from the alcoholic version. However, consumers perceived the nonalcoholic Pilsner as less bitter than its alcoholic counterpart even when IBUs were nonsignificantly different. This study emphasized the significance of understanding beer chemometrics to comprehend consumer acceptability, highlighting the crucial role of bitter molecules. Hence, hordenine, acidity, and volatile contents provided additional and valuable insights into consumer preferences.
Assessment of mental wellbeing of university students in Bangladesh using Goldberg's GHQ‐12: A cross‐sectional study
Authors: Ovi Musaddiqur R. , Siddique Md A. B. , Ahammed Tanvir , Chowdhury Muhammad A. B. , Uddin Md J. 
Published: March 2024

AbstractBackground and AimsThe mental well‐being of university students is a primary public health concern worldwide, including in Bangladesh. The objective of this study was to determine the prevalence of the overall mental health status among Bangladeshi university students. The study used larger and more diverse sample compared to previous studies, and also explored factors associated with the mental health well‐being of those students.MethodsData were collected through an online questionnaire, utilizing the proportional allocation method, from students in various universities across Bangladesh. The sample included 2036 participants. The study applied Goldberg's General Health Questionnaire (GHQ‐12) using the GHQ2+ threshold (i.e., having more than two symptoms). A binary outcome variable was created with two levels: “good mental health” and “poor mental health,” to assess the mental health status of the university students. The explanatory variables were age, gender, academic year, type of university, and sources of personal expenses. Exploratory data analysis, association tests, and binary logistic regression models were used to identify factors influencing the outcome variable.ResultsA total of 55.9% of students (male: 52.6% and female: 62.8%) exhibited poor mental health status. Female students' mental health was found to be worse (odds ratio [OR]: 1.49, 95% confidence interval [CI]: 1.23–1.81) compared to that of males. Similarly, public university students displayed a worse mental health condition than their counterparts in private universities (OR: 1.29, 95% CI: 1.03–1.61).ConclusionThe overall mental health of university students in Bangladesh is concerning. There is a pressing need for effective mental health policies and interventions to bolster the mental well‐being of university students, with a specific focus on students from public universities and females.
Experiences in the use of multiple doses of convalescent plasma in critically ill patients with COVID‐19: An early phase 1 descriptive study
Authors: Aguilar Ricardo , López‐Vergès Sandra , Quintana Anarellys , Morris Johanna , Lopez Lineth , Cooke Ana , Quiel Dimas , Buitron Natalie , Pérez Yaseikiry , Lobo Lesbia , Ballesteros Maura , Pitti Yaneth , Diaz Yamilka , Saenz Lisseth , Franco Danilo , Castillo Daniel , Valdespino Elimelec , Blanco Isabel , Romero Emilio , Villarreal Alcibiades , Cubilla‐Batista Idalina 
Published: March 2024

AbstractBackgroundAt the beginning of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) pandemic, transfusion of coronavirus disease 2019 (COVID‐19) convalescent plasma (CCP) emerged as a potential therapeutic strategy to help patients severely afflicted by COVID‐19. The efficacy of CCP has been controversial as it depends on many variables pertaining to the plasma donor and the patient with COVID‐19, for example, time of convalescence or symptoms onset. This feasibility and descriptive study aimed to assess the safety of multiple doses of CCP in mechanically ventilated, intubated patients with respiratory failure due to COVID‐19.MethodsA cohort of 30 patients all experiencing severe respiratory failure and undergoing invasive mechanical ventilation in an intensive care unit, received up to five doses of 300–600 mL of CCP on alternate days (0, 2, 4, 6, and 8) until extubation, futility, or death.ResultsNineteen patients received five doses, seven received four, and four received two or three doses. At 28‐day follow‐up mark, 57% of patients recovered and were sent home, and the long‐term mortality rate was 27%. Ten severe adverse events reported in the study were unrelated to CCP transfusion. Independent of the number of transfused doses, most patients had detectable levels of total and neutralizing antibodies in plasma.ConclusionThis study suggests that transfusion of multiple doses of CCP is safe. This strategy may represent a viable option for future studies, given the potential benefit of CCP transfusions during the early stages of infection in unvaccinated populations and in settings where monoclonal antibodies or antivirals are contraindicated or unavailable.
Reimagining bioRxiv and preprint servers as platforms for academic learning
Authors: Benjamin Jazmine , Wanjalla Celestine N. , Gaddy Jennifer A. , Kirabo Annet , Williams Edith M. , Hinton Antentor 
Published: March 2024

AbstractA popular preprint server, bioRxiv, is important as a tool for increased visibility for life science research. If used properly, however, bioRxiv can also be an important tool for training, as it may expose trainees (degree‐seeking students undertaking research or internships directly related to their field of study) to the peer review process. Here, we offer a comprehensive guide to using bioRxiv as a training tool, as well as offer suggestions for improvements in bioRxiv, including confusion that may be caused by bioRxiv articles appearing on PubMed.
Friendship fallout and bailout backlash: The psychology of borrowing and lending
Authors: Angulo Ashley N. , Goldstein Noah J. , Norton Michael I. 
Published: March 2024

AbstractSix studies explore the psychology of borrowing and lending. Across two different contexts—friends lending to friends and taxpayers bailing out businesses—lenders are angrier with borrowers who specifically make hedonic (as opposed to utilitarian) purchases with loaned money. This anger is pronounced enough that lenders' negative feelings toward borrowers who made past hedonic (vs. utilitarian) purchases remains even after they have been fully repaid. Undergirding lender anger is deserved oversight—a novel construct capturing people's belief that they deserve control and say over another's decision‐making. Borrowers and lenders do not agree on who deserves oversight over how the loaned funds are spent in large part because they differ in how much perceived ownership they each feel over the money. When lenders are yet to be repaid, their desire for oversight extends even to purchases made separately from the loaned amount. Finally, these processes and consequences are most powerful when money is lent compared with other forms of exchange, such as gifting money or being paid for work.
The molecular and metabolic events behind different germination stages of rice seeds: A metabolomics perspective
Authors: Hazra Anindita , Das Susmita 
Published: March 2024

AbstractSeed germination is a crucial stage for the completion of plants' life cycle. The process of seed germination is a very important phenomenon for agricultural food and crop production and to understand the underlying food chemistry. Rice (Oryza sativa L.) being easy to grow and cheap source of phytonutrients is a fundamental food crop across the world. Several researches are going on for years to improve the production of rice, ameliorate the deadly bacterial or fungal diseases utilizing genetics, transcriptomics, and proteomics aspects. Metabolomics is a very new arena of research in this field. With the help of modern‐day metabolomic tools and technologies, researchers are incessantly trying to unveil the metabolic construction of rice seeds, and new to this topic is seed germination. The principal aim of this review on metabolomics of germinating rice seeds is to have a big picture on the key molecular, cellular, and metabolic events of germination. This review tries to summarize all the available data to fulfill the aforesaid aim.
Determination, distribution, and health risk assessment of 12 heavy metals in various edible oils in Taiwan
Authors: Lin Shu‐Ting , Liao Kai‐Wei , Xuan Tan‐Chui , Chiou Tai‐Ying , Lin Zih‐Ee , Lee Wei‐Ju 
Published: March 2024

AbstractBackgroundMinimizing the risk of heavy metal pollution is of utmost importance to ensure consumer safety. In this research, we utilized inductively coupled plasma‐mass spectrometry to determine the presence of 12 heavy metals in Taiwanese commercial edible oils, both refined and unrefined.ResultsOur analytical findings revealed significantly lower levels of heavy metals in refined oils compared with unrefined oils. Through principal component analysis and cluster analysis of 19 types of edible oils, we identified Cd, Ba, Fe, Pb, and Co as the primary variables influencing heavy metal levels in edible oils. Notably, the heavy metal contents in the edible oils did not surpass international standards. Risk assessment results indicated that unrefined oils carried higher risk levels than refined oils. The incremental lifetime cancer risk values calculated for unrefined oils exceeded 10−6 in all age groups except for the 19 ~ 65 years category, while the noncarcinogenic risks remained well below 1, regardless of refined or unrefined oils.ConclusionsOur study sheds light on the distribution characteristics of heavy metal contents in edible oils, highlighting higher levels of heavy metals and associated risks in non‐refined oils compared with refined oils. Ultimately, the presence of heavy metals in edible oils poses low health risks to the human body.
Tri‐frequency simultaneous ultrasound pickling for the acceleration of the NaCl content and quality improvement of pork (longissimus dorsi)
Authors: Zhang Xinyan , Guo Lina , Chen Zhongyuan , Ma Haile 
Published: March 2024

AbstractBACKGROUNDThe pickling process with NaCl is an essential step for pork preservation. This study aimed to investigate the effect of different ultrasonic intensities of tri‐frequency simultaneous ultrasound (TSIU) pickling on the NaCl content and quality of pork (longissimus dorsi). After 30 min pickling, the NaCl content, moisture content, pickling yield, cooking loss, textural properties, color, pH, moisture migration and distribution as well as microstructure of pork were assessed.RESULTSResults showed that among all the ultrasonic treatment intensities (85–150 W L−1), the NaCl content of the sample pickled by an intensity of 101.3 W L−1 was higher than that of other intensities. TSIU 101.3 W L−1 showed 59.95% higher NaCl content than the control sample. In addition, the sample treated with TSIU of 101.3 W L−1 had higher pickling yield and moisture content, better textural properties of pork (including hardness and chewiness), and less cooking loss. The results of the low‐field nuclear magnetic resonance showed that, compared with the control group, the relaxation time T21 of the ultrasound‐assisted pickling samples increased, while the proportion of T22 (A22) reduction ranged from 175.0% to 379.9%. The microstructure designated that the ultrasonic treatment could facilitate changes in meat texture.CONCLUSIONUltrasound marination of different intensities promoted the diffusion of NaCl and affected the quality of pork tenderloins. The TSIU at 101.3 W L−1 could better accelerate NaCl transport and homogeneous distribution on meat, thereby improving the sample quality. © 2024 Society of Chemical Industry.
Movement Disorder Perspectives on Monocarboxylate 8 Deficiency: A Case Series of 3 Colombian Patients with Allan–Herndon–Dudley Syndrome
Authors: Ramon‐Gomez Jorge Luis , Cortés‐Rojas Maria Camila , Polania‐Puentes Maria Jose , Guerrero‐Ruiz Graciela Del Pilar 
Published: March 2024

AbstractBackgroundDeficiencies in the thyroid hormone transporter monocarboxylate 8 (MCT8) due to pathogenic variants in the SLC16A2 gene (OMIM 300095) result in a complex phenotype with main endocrine and neurologic symptoms. This rare disorder, named Allan–Herndon–Dudley syndrome (AHDS) (OMIM 300523), is inherited in an X‐linked trait. One of the prominent features of AHDS is the presence of movement disorders (MD), which are complex and carry a significant burden of the disease.CasesPatient 1: male with hypotonia since birth, developmental delay, dystonic posturing at 4 months and at 15 months, and startle reaction developed with sensory stimuli. Patient 2: male, at 2 months, shows hypotonia and developmental delay, paroxysmal episodes triggered by a stimulus with sudden blush, tonic asymmetric posture, and no epileptiform activity. At 10 months, generalized dystonic posturing. Patient 3: typical neurodevelopmental milestones until 6 months; at 24 months, dystonia, startle reaction, and upper motoneuron signs.ConclusionsWe aim to describe our patients diagnosed with AHDS, focusing on MD phenomenology and strengthening the phenotype–genotype correlations for this rare condition.
The factors influencing patient safety management as perceived by emergency department nurses: A qualitative study
Authors: Mohammadi Fateme , Rustaee Sanaz , Bijani Mostafa 
Published: March 2024

AbstractAimOne of the most important, unpredictable and stressful areas in hospitals is the emergency department (ED) where seconds are crucial for providing immediate care and saving the patients' lives. Therefore, the present study aimed to identify the factors which impact the patient safety management as perceived by the ED nurses in Southern Iran.DesignThis is a qualitative, descriptive study.MethodsThe participants were 23 ED nurses selected via purposeful sampling who were asked to take part in an interview. Data were collected using semi‐structured, individual, in‐depth interviews and analysed via content analysis.ResultsAnalysis of the qualitative data yielded 4 themes and 12 subthemes. The four main themes were: negligence of safety standards and standard precautions, disregard of ethical principles, professional challenges and inefficient organizational management.