bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Malocclusion Types in Adult Patients with Nasal Septal Defects – An Observational Cross-Sectional Analysis
Authors: Ganesan Poornima , Golla Usha Rao , Balashanmugam Baskaranarayanan , Munuswamy Geetha Lakshmi 
Published: April 2024

ABSTRACT

Introduction:
The nasal septum is crucial in the development of the craniofacial structures. Deviated nasal septum is one of the anatomical causes of mouth breathing which in turn lead to malocclusion.

Aims and Objectives:
To evaluate the dentofacial and cephalometric characteristics in individuals with nasal breathing obstruction brought on by nasal septal deviation, as well as the relationship between these defects and various malocclusions and the degree of facial asymmetry.

Materials and Methods:
A two-point evaluation was adopted for the selected patients, one at the ENT department using clinical examination and CT–PNS and the severity classified according to the Mladina classification and another at the dental department, using clinical examinations, PA cephalograms, lateral cephalograms, and facial photographs.

Results and Discussion:
The association between malocclusion and various grades of septal deviation was statistically significant with a P value of 0.006. Results showed that 13 patients are with Class I skeletal pattern, 20 patients with Class II, and 7 patients with Class III skeletal pattern. None of the grade 7 nasal septal deviation patients had class I malocclusion and none of the grade 2 nasal septal deviation patients had class III malocclusion. Class II division I malocclusion was the most common type noted in patients with nasal septal deviation. Grade 7 nasal septal deviation was associated with the maximum amount of ANS and mentioned deviation indicating significant facial asymmetry.

Conclusion:
Class-II Division-1 malocclusion was the most common type noted and Class III malocclusion was more common in higher grades of nasal septal deviation. Maxillary and mandibular asymmetry worsens significantly with an increase in the grade of nasal septal deviation and is one of the significant factors in causing facial asymmetry.
Evaluation of Different Root Canal Sealers on Post Operative Pain in Single Visit RCT – A Comparative Study
Authors: Saini Ankita , Dheeraj Munish , Johar Suksham , Goyal Shweta , Chahal Simran , Garg Dhruvika 
Published: April 2024

ABSTRACT

Aim:
To evaluate and compare three different root canal sealers (Endo-Sequence BC, Bio Root RCS and Zinc Oxide Eugenol) on post operative pain in single visit root canal therapies.

Material and Methodology:
60 subjects with age ranging from 18-60 years were randomly selected depending upon the inclusion criteria. The subjects were then divided into three groups with 20 patients in each. In all the patients’ single visit root canal treatment was done followed by obturation using different sealers. In Group I Endo- Sequence sealer was used, in Group II Bio-Root RCS and in Group III Zinc Oxide Eugenol sealers were used respectively.

Results:
statistically significant results were obtained on comparing the efficacy of three groups with Group I proving to be much effect among the three.

Conclusion:
within the limitation of the study it can be concluded that though all the sealers were effective in reducing post-operative pain, patients with Endo Sequence BC sealers evaluated statistically significant results.
WhatsApp as a Tool in Blended Learning in Dental Education
Authors: Prasad Prathibha , Gunasekaran Lalithambigai , Khair Al Moutassem B. , Shetty Raghavendra , Shetty Naresh 
Published: April 2024

ABSTRACT

Background and Objective:
Dental education is unique, with clinical/practical learning much different from other professionals. In dentistry, students are exposed much earlier in their curriculum to patients, performing procedures under direct/indirect supervision. Hence, the present review attempted to assess the standing position of dentistry in blended learning worldwide.

Materials and Methods:
E-learning modality was at its primitive stage as food for thought among educational task force committees, with only a handful of developed and developing countries equipped with it. During this pandemic, “WhatsApp” became a savior, as it was a widely used educational device among dental teachers and students. Furthermore, it was easily applicable in places where there was difficulty in obtaining regular internet bandwidth, especially in remote/rural areas. It is noteworthy that blended learning/e-learning, when complemented with the conventional teaching method, upskill critical thinking and problem-solving capabilities. Blended learning or virtual reality education should be made feasible without disparity, worldwide. Mind training, both students and staff in hybrid e-learning, is the need of the hour not only to keep pace with digitalized dentistry but to prepare the students to face real-world situations.

Conclusion:
The sustainability of dental education should be rethought by dental schools with “Complimented Digital learning” wherever feasible, not only to cope with current trends but also to face unforeseen future challenges.
Antimicrobial Activity of Crude Metabolites of Vitis vinifera using Methanol Extract against the Clinical Pathogens
Authors: Karkuzhali Karunanithi , Manivannan N. , Venkatesan Santhosh 
Published: April 2024

ABSTRACT

Background:
The Vitis vinifera, also known as grapevine, is one of the most widely grown fruit crops in the world and is renowned for producing wine and grapes. Other than their importance in gastronomy and ecology, certain sections of the V. vinifera plant have shown promising bioactive qualities. The numerous phytochemicals in this plant, including flavonoids, stilbenes, and phenolic acids, are what gives its antibacterial and antifungal properties though the antimicrobial properties of seed extract have to be studied, however in this present study we focus on screening and its biological compatibility of seed extracts of V. vinifera.

Methods:
The commercial power of seed (V. vinifera) obtained from local market near Poonamallee, Chennai, India. and the extraction of crude metabolites was done by direction extraction method, the antimicrobial activity was done by well diffusion method, and Minimum Inhibitory concentration was done by CLSI guideline. To check the biocompatibility of crude metabolites was done by hemolytic assay.

Results:
Studies have demonstrated that grapevine extracts and their separated components have potent antibacterial and antifungal effects against a variety of pathogenic microorganisms, including bacterial strains that are resistant to antibiotics. The Minimum Inhibitory Concentration of the plant’s extracts have demonstrated potential 128 µg/mL for S. aureus, and 256 µg/mL E. faecalis and C. albicans as the best inhibitory concentration. The biological compatibility of crude metabolites shows 3 % of lysis at 512 µg/mL.

Conclusion:

V. vinifera is a prospective source for the creation of novel antimicrobial drugs because of its antibacterial capabilities. To completely understand the chemicals’ mode of action and to create efficient treatments for microbial illnesses, more research is necessary.
Evaluation of In vitro Anti-Cancer Activity of Methanolic Leaf Extract of Phoenix pusilla on Colon Cancer Cell Line
Authors: Bharadwaj Nikhil , Manimuthu Mani Sankar , Vimal S. , Radhakrishnan N. 
Published: April 2024

ABSTRACT

Background:
Cancer rates continue to climb, owing largely to the world population’s aging and growth, as well as economically developing countries, a surge in cancer-causing behavior, particularly smoking. The third or fourth most prevalent type of cancer is colon cancer. Cancer of the large intestine (colon) is one of the primary causes of death from cancer. Colorectal cancer prevention is mostly based on adenomatous disease screening approaches. The cytotoxic and pharmacological properties of Phoenix pusilla are widely documented. As a result, there is little recorded evidence of its cytotoxic activity against colon cancer cells. Therefore, we planned to study the efficacy of a methanolic leaf extract of Phoenix pusilla against in vitro colon cancer cells.

Aim:
To evaluate the anti-cancer effects of the methanolic leaf extract of Phoenix pusilla on colon cancer cell lines.

Materials and Methods:

In vitro screening and anti-cancer effects of the methanolic effect of Phoenix pusilla on colon cancer cell lines were assessed by cell viability assays and cell and nuclear morphological studies. For the in vitro cell culture study, different concentrations of Phoenix pusilla leaf extract (0, 25, 50, 75, 100, 150 μg/ml) were used, and IC50 doses were calculated.

Results:
The results of the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay revealed that the fraction of viability cells significantly decreased in treated cells when compared to untreated control groups, was expressed as 100%, and an inhibitory concentration of μg/ml was identified. A phase-contrast microscope was used to observe cell shrinkage and cytoplasmic membrane blebbing. A fluorescent microscope was used to examine the apoptotic nuclei (internally dyed nuclei, shattered nuclei, and condensed chromatin).

Conclusion:
In conclusion, the present study results showed that the leaf extracts of Phoenix pusilla had a strong cytotoxic effect and induced significant apoptosis in the colon cancer cell lines at a concentration of 75 μg/ml in the 24 h incubation period. More research is needed to investigate the extract’s active components as well as the molecular mechanisms underlying its anti-cancer properties.
Studies on Anticancer Effect of Methanolic Leaf Extract of Justicia gendarussa on Lung Cancer Cell Line
Authors: Lavanya Ravi , Vimal S 
Published: April 2024

ABSTRACT

Objective:
The aim of study’s goal was to look into the anticancer efficacy of a methanolic extract of Justicia gendarussa against a lung cancer cell line.

Materials and Methods:
Cell viability assays and cell and nuclear morphology examinations were used to evaluate the anticancer efficacy against methanolic extract of Justicia gendarussa on lung cancer cell lines. The IC50 doses were calculated using different concentrations of Justicia gendarussa extract (0, 10, 20, 40, 60, and 80 μg/mL).

Results:
The results of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay revealed that the percentage of viability in treated cells was significantly lower as compared with untreated control groups, which represented as 100%, and an inhibitory concentration of 40 μg/mL was observed. Under a phase-contrast microscope, morphological changes revealed cell shrinkage and cytoplasmic membrane blebbing. The apoptotic nuclei (intensely colored, broken nuclei, and compacted chromatin) were examined under a fluorescence microscope.

Conclusions:
The outcome of the research work on Justicia gendarussa was investigated for anticancer properties. The results revealed the proapoptotic and cytotoxic effects of Justicia gendarussa extract on lung cancer cell lines. From the above results and findings, it could be concluded that the Justicia gendarussa methanolic leaf extract exhibited potent anticancer activity against a lung cancer cell line. Further study needs to be conducted to investigate the active chemicals in the extract as well as the molecular mechanisms underlying its anticancer benefits.
Phytosynthesis of Eco-Friendly Silver Nanoparticles Using Lawsonia Innermis (L) and Their Biomedical Applications
Authors: Senthilkumaran Soowrish 
Published: April 2024

ABSTRACT

Background:
The present study, plant extract to biosynthesize silver nanoparticles (AgNPs), is an environmentally benign way to lessen the use of dangerous chemicals.

Aims and Objectives:
The antibacterial effects of the green production of AgNPs by Lawsonia inermis extract were examined.

Materials and Methods:
Utilizing scanning, transmission electron microscopy, X-ray diffraction (XRD), ultraviolet-visible spectroscopy, and infrared spectroscopy, researchers examined the physical and chemical characteristics of synthesized AgNPs.

Results:
Ag-NPs have the highest peak in visible light at 460 nm, according to UV-vis analysis. When silver nanocrystals were structurally characterized, peaks that matched Bragg’s diffractions were found, with average crystallite sizes ranging from 28 to 60 nm. Examining Ag-NPs’ antibacterial properties, it was shown that all microbes are extremely sensitive to these biologically produced Ag-NPs.

Conclusion:

Escherichia coli, Salmonella typhi, Bacillus cereus, and Staphylococcus aureus were tested for the antimicrobial properties of AgNPs synthesized.
Cytoplasmic Leakage and Protein Leakage Analysis of Ocimum Gratissimum Stem Extract-Mediated Silver Nanoparticles Against Wound Pathogens
Authors: Shalini V. , Shanmugam Rajeshkumar , Manigandan Pradeep 
Published: April 2024

ABSTRACT

Aim:
The current work intends to examine the antibacterial activity of silver nanoparticles (AgNPs) mediated by Ocimum gratissimum stem extract against wound infections.

Materials and Methods:
To evaluate the membrane damage brought on by AgNPs, analyses of cytoplasmic leakage and protein leakage assays were performed.

Results:
The outcomes demonstrated that all of the tested bacterial strains were significantly resistant to the AgNPs’ antibacterial activity. AgNPs damaged membranes and caused cellular contents to leak in the target pathogens, according to an examination of protein and cytoplasmic leakage.

Conclusion:
According to the current investigation, AgNPs mediated by Ocimum gratissimum stem extract may be effective antibacterial agents against microorganisms that cause wounds.
Phytochemical Screening and Antidiabetic Activity of Aqueous Extract of Evolvulus Alsinoides Leaves: An In Vitro and In Silico Study
Authors: Mukundh S Tarun , Veeraraghavan Vishnu Priya , Ponnusamy Bhuvaneswari , Jayaraman Selvaraj 
Published: April 2024

ABSTRACT

Background:
Nowadays, diabetes mellitus has become common worldwide due to changes in lifestyle, sedentary life, alterations in food habits, and other genetic and environmental factors. It is necessary to create awareness about the growing pandemic.

Aim:
To evaluate the antidiabetic potential of Evolvulus alsinoides.

Methods:

In vitro α-amylase inhibition and α-glucosidase inhibition activity. Molecular docking analysis.

Results:
The plant is rich in naturally occurring phytocompound. The results of the study showed that E. alsinoides has the potential to inhibit the activity of alpha-amylase and alpha-glucosidase. Moreover, this result was validated using in silico molecular docking studies that showed a good binding affinity of one of the major phytocompound, caffeic acid, with molecules in the insulin signaling cascade.

Conclusion:

E. alsinoides has a potent antidiabetic activity and can be further employed in animal and human studies.
Green Synthesis of Mimosa pudica-Mediated Strontium Nanoparticles and its Anti-Inflammatory Activity
Authors: Harshini Sakthi , Shanmugam Rajeshkumar , Govindharaj Sulochana 
Published: April 2024

ABSTRACT

Background:
Considerable focus has been directed toward green synthesis as a dependable, sustainable, and environmentally friendly approach for synthesizing various nanomaterials. Mimosa pudica, a quickly grown pantropical weed, has been used widely for its anti-inflammatory and antimicrobial activity in traditional medicine. The development of strontium-based nanoparticles and nanoparticles linked with strontium has garnered attention in recent years due to their established utility in diverse domains such as effective drug distribution, bioimaging, cancer treatment, and advancements in bone engineering.

Aims and Objectives:
To examine the green synthesise of strontium nanoparticles using Mimosa pudica and its anti-inflammatory activity.

Material and Methods:

Mimosa pudica-mediated strontium nanoparticles’ anti-inflammatory activity was tested using bovine serum albumin denaturation assay, egg albumin denaturation assay, and membrane stabilization assay with diclofenac sodium as the standard. Result: In all three assays, increasing concentration of Mimosa pudica-mediated strontium nanoparticles exhibited an increasing anti-inflammatory effect, which was similar to the standard diclofenac sodium.

Conclusion:
Consequently, this holds promise as a new potential anti-inflammatory agent in forthcoming applications.
Antioxidant and Anti-Inflammatory Activity of the Ethanolic Extract of Euphorbia Hirta Leaf Extract: An In Vitro and In Silico Study
Authors: Praveen G , Krishnamoorthy Kaliselvi , Veeraraghavan Vishnu Priya , Jayaraman Selvaraj 
Published: April 2024

ABSTRACT

Objectives:
This study presents the antioxidant and anti-inflammatory activity of the ethanolic extract of Euphorbia hirta leaf extract.

Materials and Methods:
The antioxidant and anti-inflammatory activity of the extract was performed by in vitro assay. Our research employs a comprehensive approach combining experimental assays and computational simulations to assess the extract’s potential bioactive components and their interactions with key biomolecules.

Results:
The study’s results demonstrated a progressive rise in the percentage of inhibition, which was dependent on the dosage, in both antioxidant and anti-inflammatory activities. This trend was observed for both the extract and the standard, encompassing concentrations ranging from 100 to 500 μg/ml.

Conclusion:
The results showed that Euphorbia hirta’s possesses antioxidant and anti-inflammatory activity, and this may contribute to a traditional medicinal. The discoveries of this study contribute to a deeper understanding of Euphorbia hirta’s medicinal properties and its potential as a source of natural therapeutic agents.
Phytochemical Screening and Anti-Inflammatory and Anti-Oxidant Activities of Spermacoce Hispida Ethanolic Extract
Authors: Nithin Krishna K. , Krishnamoorthy Kalaiselvi , Veeraraghavan Vishnu Priya , Jayaraman Selvaraj 
Published: April 2024

ABSTRACT

Background:

Spermacoce hispida, a medicinal plant from the Rubiaceae family, has garnered attention for its traditional use and reported therapeutic properties. This study aimed to investigate the phytochemical composition and assess the anti-inflammatory and anti-oxidant activities of the ethanolic extract derived from Spermacoce hispida.

Materials and Methods:
Phytochemical screening of the ethanolic extract involved qualitative analysis to identify major phytoconstituents such as tannins, phenols, and acids. The anti-inflammatory activity was evaluated through in vitro assays, and anti-oxidant potential was assessed using established methods to measure scavenging activity against free radicals and reactive oxygen species.

Results:
Phytochemical screening revealed the presence of various bioactive constituents in the Spermacoce hispida ethanolic extract, including acid, tannin, protein, and phenolic compounds. Furthermore, the extract exhibited potent anti-oxidant activity, as evidenced by its ability to scavenge free radicals and attenuate ROS-induced oxidative stress.

Conclusion:
The findings of this study underscore the potential of Spermacoce hispida ethanolic extract as a source of bioactive compounds with anti-inflammatory and anti-oxidant properties. The presence of phytochemicals and the observed bioactivity support its traditional use and suggest potential therapeutic applications. These results contribute to the growing body of knowledge on natural products with health-promoting effects and provide a basis for further research aimed at developing pharmaceutical and medicinal interventions harnessing the benefits of Spermacoce hispida.
Accuracy of 3D Printed Model Acquired from Different Types of Intra Oral Scanners and 3D Printers
Authors: Joteppa Varsha , Niras Soniya , Chokhani Dishita , Jadhao Tanvi A. , Bandgar Shreyas T. , Bayaskar Surbhi G. 
Published: April 2024

ABSTRACT

Objective:
The aim of this study was to verify the influence of different types of intraoral scanners and 3D printers on the accuracy of printed models in comparison to plaster models obtained from conventional impressions.

Material and Methods:
A dental study model was used as the reference model and was molded with polyvinyl siloxane to produce the plaster models which were scanned by a reference scanner. Two types of intraoral scanners and digital files were printed by two types of 3D printers. To measure the accuracy (trueness and precision) amongst the groups, the datasets were superimposed via a best-fit alignment method utilizing a 3D analysis program (Geomagic Verify; 3D Systems). The trueness of the complete arch was evaluated by superimposing the STL file data of the reference model with STL file data obtained from other scanners. The precision of the complete arch was evaluated by superimposing the scan data within each group. The quantitative values were automatically calculated by the 3D analysis program based on the root mean square (RMS).

Results:
It was observed that all the tested combinations of the scanner and 3D printer showed variation from reference which was nonsignificant. However, Trios 4 intraoral scanner and Formlabs 3D printer was the combination that showed the best trueness and precision values.

Conclusions:
It was concluded that the accuracy of printed and plaster models was impaired due to the trueness of the models. The type of printer influenced the accuracy of the printed models, while the type of scanner did not. The standardization of the method of obtaining printed models must be carried out to provide the production of quality models. However, there will be differences between the technologies.
Efficacy of TheraCal LC and Biodentine as Direct Pulp Capping Agents - An Clinico-Histological Study
Authors: Singh Shalini , Joshi Poonam , Rajasekhar Reshma , Saoji Sumant V. , Richhawal Anil , Punia Jasleen K. 
Published: April 2024

ABSTRACT

Aim:
To assess clinically and histologically the efficacy of TheraCal LC and Biodentine as Direct Pulp Capping agents.

Materials and Method:
Sixty caries-free maxillary first premolar teeth which were scheduled for orthodontic extraction were selected for the study and were divided equally into Group 1 (Theracal LC) and Group 2 (Biodentine). Thermal and electric pulp testing was performed on the teeth to assess pulp vitality followed by tooth preparation to expose pulp horns under local anesthesia. After achieving hemostasis, the direct pulp capping agent was placed and composite restoration was done. All the teeth were evaluated and compared in terms of clinical and histological findings after 6 weeks. The statistical analysis used was the Kruskal-Wallis test.

Results:
Teeth with Theracal LC showed no sensitivity to heat, cold, or percussion, whereas sensitivity to heat, cold, or percussion was noted with Biodentine. Histological evaluation revealed that TheraCal LC has higher efficiency in complete dentin bridge formation, had maximum dentin thickness, and showed minimal or no pulpal disorganization and pulpal inflammation as compared to Biodentine.

Conclusion:
TheraCal LC proves to be a promising future as a direct pulp capping agent.