bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of a questionnaire to identify barriers to the implementation of the Clinical Practice Guidelines for lower limb amputees in a middle‐income country
Authors: Posada‐Borrero Ana‐Maria , Plata‐Contreras Jesús , Lugo‐Agudelo Luz Helena , Velásquez‐Correa Juan Carlos , Patiño‐Lugo Daniel F. , del Pilar Pastor‐Durango Maria , Aguirre‐Acevedo Daniel Camilo 
Published: March 2024

AbstractAimTo develop and validate a questionnaire to identify the perceived barriers in the implementation of the Clinical Practice Guidelines for the lower limb amputee (CPG‐AMP).Study Design and SettingThe study consisted of two stages: first, the development of the questionnaire based on a meta‐review of the literature and interviews with patients and health providers (mixed‐methods research). Second, the evaluation of its psychometric properties was performed. Participants included health providers from hospitals and clinics, prosthetic workshops and academic institutions in Colombia.ResultsA total of 90 items were obtained from the literature review and interviews. The validation of a preliminary 66‐item questionnaire was performed with 545 participants. After the factorial analysis, a 25‐item questionnaire with four domains was developed. Internal consistency was adequate in all domains, with Cronbach's α values between 0.76 and 0.83. Test–retest reliability in 58 participants yielded intraclass correlation coefficients between 0.51 and 0.59.ConclusionsA 25‐item questionnaire with four domains (health system, guideline, institutional and individual) was proposed to measure the perception of barriers to the CPG‐AMP. The conceptual framework and the questionnaire can be used to identify barriers of other CPGs and to help design strategies aimed at improving its implementation.
Sex differences in cognitive function trajectories and their determinants in older adults: Evidence from the Chinese longitudinal healthy longevity survey
Authors: Tu Lihui , Lv Xiaozhen , Yuan Changzheng , Chen Hui , Yu Xin , Wang Huali , Zhang Qinge 
Published: March 2024

AbstractObjectivesTo examine sex differences in the cognitive trajectories of a nationally representative sample of older people living in China and to explore potential determinants of these trajectories.MethodsThe study included 2230 women and 2171 men who were cognitively healthy and aged over 60 at the first observation from the Chinese Longitudinal Healthy Longevity Survey based on the 2008–2018 cohort. Cognitive function was measured using the Chinese version of the Mini‐Mental State Examination (MMSE). Group‐based trajectory modeling was used to identify potential heterogeneity of longitudinal changes over the 10 years in each gender. Logistic regression was used to investigate associations between baseline characteristics (age, education, fertility history, sleep length, physical activity, and health status and behaviors) and trajectory classes.ResultsThree trajectories (labeled stable, slow decline, and rapid decline) were identified according to the changes in MMSE scores for both women and men. For the women, both the slow and rapid decline groups accounted for a larger proportion (14.7% and 11.0%, respectively) than the male decline groups (8.1% and 6.6%, respectively), and the women had a lower baseline MMSE score with a faster decline. In the multivariable logistic regression analyses, older age, less education, older age at first birth, poorer functional abilities, hearing impairment, and lower baseline MMSE scores were significantly associated with cognitive decline in both the female and male groups compared to the stable group. For the women, sleeping over 9 h was also associated with a rapid cognitive decline trajectory, while current exercise and being overweight/obese were protective factors against cognitive decline.ConclusionsThe women had an overall more serious cognitive decline than men. The potential determinants of cognitive decline identified in this study could be considered for developing specific intervention strategies aimed at promoting a healthy brain and preventing cognitive decline in different sexes, especially in low‐income and developing countries.
General and specific patterns of cortical gene expression as spatial correlates of complex cognitive functioning
Authors: Moodie Joanna E. , Harris Sarah E. , Harris Mathew A. , Buchanan Colin R. , Davies Gail , Taylor Adele , Redmond Paul , Liewald David C. M. , Valdés Hernández Maria del C. , Shenkin Susan , Russ Tom C. , Muñoz Maniega Susana , Luciano Michelle , Corley Janie , Stolicyn Aleks , Shen Xueyi , Steele Douglas , Waiter Gordon , Sandu Anca‐Larisa , Bastin Mark E. , Wardlaw Joanna M. , McIntosh Andrew , Whalley Heather , Tucker‐Drob Elliot M. , Deary Ian J. , Cox Simon R. 
Published: March 2024

AbstractGene expression varies across the brain. This spatial patterning denotes specialised support for particular brain functions. However, the way that a given gene's expression fluctuates across the brain may be governed by general rules. Quantifying patterns of spatial covariation across genes would offer insights into the molecular characteristics of brain areas supporting, for example, complex cognitive functions. Here, we use principal component analysis to separate general and unique gene regulatory associations with cortical substrates of cognition. We find that the region‐to‐region variation in cortical expression profiles of 8235 genes covaries across two major principal components: gene ontology analysis suggests these dimensions are characterised by downregulation and upregulation of cell‐signalling/modification and transcription factors. We validate these patterns out‐of‐sample and across different data processing choices. Brain regions more strongly implicated in general cognitive functioning (g; 3 cohorts, total meta‐analytic N = 39,519) tend to be more balanced between downregulation and upregulation of both major components (indicated by regional component scores). We then identify a further 29 genes as candidate cortical spatial correlates of g, beyond the patterning of the two major components (|β| range = 0.18 to 0.53). Many of these genes have been previously associated with clinical neurodegenerative and psychiatric disorders, or with other health‐related phenotypes. The results provide insights into the cortical organisation of gene expression and its association with individual differences in cognitive functioning.
Online ratings and narrative comments of American Head and Neck Society surgeons
Authors: Kim Joshua K. , Tawk Karen , Kim Jonathan M. , Shahbaz Hady , Lipton Joshua A. , Haidar Yarah M. , Tjoa Tjoson , Abouzari Mehdi 
Published: March 2024

AbstractBackgroundWe analyzed online rating scores and comments of head and neck surgeons to understand factors that contribute to higher ratings.MethodsNumerical ratings and comments for American Head and Neck Society physicians were extracted from Healthgrades, Vitals, RateMDs, and Yelp, with narrative comments categorized based on content. Physician practice location, education, and residency training were also compiled.ResultsPatient ratings were significantly higher with supportive staff and affable physician demeanor but showed significant drops with longer wait times and difficulties scheduling appointments or follow‐ups. Physician education and postgraduate training did not significantly affect ratings.ConclusionOnline ratings and comments correlated to modifiable factors in clinical practice and may be informative in understanding patient needs.
Comparison of the prognostic value of lymph node yield, lymph node ratio, and number of lymph node metastases in patients with oral squamous cell carcinoma
Authors: Struckmeier Ann‐Kristin , Buchbender Mayte , Lutz Rainer , Agaimy Abbas , Kesting Marco 
Published: March 2024

AbstractBackgroundThe aim of this study was to assess the prognostic significance of lymph node yield (LNY), lymph node ratio (LNR), and the number of lymph node metastases (LNMs) in patients affected by oral squamous cell carcinoma (OSCC).MethodsThe study included patients who underwent surgical treatment for primary OSCC. Receiver operating characteristic curves were generated to determine the optimal threshold values. Kaplan–Meier curves were employed, along with the log‐rank test, for the analysis of survival. To compare the performance in terms of model fit, we computed Akaike's information criterion (AIC).ResultsThis study enrolled 429 patients. Prognostic thresholds were determined at 22 for LNY, 6.6% for LNR, and 3 for the number of LNMs. The log‐rank test revealed a significant improvement in both overall survival and progression‐free survival for patients with a LNR of ≤6.6% or a number of LNMs of ≤3 (p < 0.05). Interestingly, LNY did not demonstrate prognostic significance. The AIC analyses indicated that the number of LNMs is a superior prognostic indicator compared to LNY and LNR.ConclusionsIncorporating LNR or the number of LNMs into the TNM classification has the potential to improve the prognostic value, as in other types of cancers. Particularly, the inclusion of the number of LNMs should be contemplated for future N staging.
Darolutamide does not interfere with OATP‐mediated uptake of docetaxel
Authors: Buck Stefan A. J. , Talebi Zahra , Drabison Thomas , Jin Yan , Gibson Alice A. , Hu Peng , de Bruijn Peter , de Ridder Corrina M. A. , Stuurman Debra , Hu Shuiying , van Weerden Wytske M. , Koolen Stijn L. W. , de Wit Ronald , Sparreboom Alex , Mathijssen Ron H. J. , Eisenmann Eric D. 
Published: March 2024

AbstractThe addition of darolutamide, an androgen receptor signalling inhibitor, to therapy with docetaxel has recently been approved as a strategy to treat metastatic prostate cancer. OATP1B3 is an SLC transporter that is highly expressed in prostate cancer and is responsible for the accumulation of substrates, including docetaxel, into tumours. Given that darolutamide inhibits OATP1B3 in vitro, we sought to characterise the impact of darolutamide on docetaxel pharmacokinetics. We investigated the influence of darolutamide on OATP1B3 transport using in vitro and in vivo models. We assessed the impact of darolutamide on the tumour accumulation of docetaxel in a patient‐derived xenograft (PDX) model and on an OATP1B biomarker in patients. Darolutamide inhibited OATP1B3 in vitro at concentrations higher than the reported Cmax. Consistent with these findings, in vivo studies revealed that darolutamide does not influence the pharmacokinetics of Oatp1b substrates, including docetaxel. Docetaxel accumulation in PDX tumours was not decreased in the presence of darolutamide. Metastatic prostate cancer patients had similar levels of OATP1B biomarkers, regardless of treatment with darolutamide. Consistent with a low potential to inhibit OATP1B3‐mediated transport in vitro, darolutamide does not significantly impede the transport of Oatp1b substrates in vivo or in patients. Our findings support combined treatment with docetaxel and darolutamide, as no OATP1B3 transporter based drug–drug interaction was identified.
Treatment of Mycoplasma genitalium infection in pregnancy: A systematic review of international guidelines
Authors: Drew Richard J. , Eogan Maeve 
Published: March 2024

AbstractBackgroundMycoplasma genitalium is an emerging pathogen, which has been linked to cervicitis, urethritis and pelvic inflammatory disease (PID). With the advent of multiplex polymerase chain reaction (PCR) panels for sexually transmitted infections, it is increasingly being identified in pregnant women.ObjectivesThe aim was to review international guidelines, which had explicit recommendations for treatment of M. genitalium infection in pregnancy and breastfeeding.Search StrategyPubMed, EMBASE and Cochrane databases were reviewed with no age, species, language or date restrictions.Selection CriteriaStudies were included if they had an explicit recommendation for treatment of M. genitalium in pregnancy. Studies were excluded if there was no recommendation in pregnancy, if they referred to other international guideline recommendations or were historical versions of guidelines.Data Collection and AnalysisReferences were manually reviewed and 50 papers were selected for review. Only four guidelines were included in the final analysis and they were from Europe, UK, Australia and Aotearoa New Zealand.Main ResultsAll studies recommended azithromycin as first‐line treatment, and advised against moxifloxacin use. The dosing schedule of azithromycin, varied between guidelines, as did the utility/safety of pristinamycin for macrolide resistant infections. Safety data was generally reassuring for azithromycin but inconsistent for pristinamycin.ConclusionsAzithromycin is the first‐line treatment for macrolide susceptible or unknown resistance infections, but there is a lack of consistency regarding dosing of azithromycin or the utility/safety of pristinamycin for macrolide resistant infections in pregnancy/lactation.
Are international guideline recommendations for the management of placenta accreta spectrum applicable in low‐ and middle‐income countries?
Authors: Nieto‐Calvache Albaro Jose , Jauniaux Eric , Fox Karin A. , Maya Juliana , Stefanovic Vedran , Weizsäcker Katharina , van Beekhuizen Heleen , Adu‐Bredu Theophilus , Collins Sally , Siaulys Monica , Hussein Ahmed M. , Duvekot Johannes , Aryananda Rozi , Pajkrt Eva , Rijken Marcus J. ,   the International Society for PAS (IS‐PAS) LMIC Working Group
Published: March 2024

AbstractObjectiveThe aim of this study was to explore how obstetricians‐gynecologists in low‐ and middle‐income countries (LMICs) can apply current international clinical practice guidelines (CPGs) for the management of placenta accreta spectrum (PAS) in limited resource settings.MethodsThis was an observational, survey‐based study. Clinicians with expertise in managing patients with PAS in LMICs were contacted for their evaluation of the recommendations included in four PAS clinical practice guidelines.ResultsOut of the 158 clinicians contacted, we obtained responses from 65 (41.1%), representing 27 middle income countries (MICs). The results of this survey suggest that the care of PAS patients in middle income countries is very different from what is recommended by international CPGs. Participants in the survey identified that their practice was limited by insufficient availability of hospital infrastructure, low resources of local health systems and lack of trained multidisciplinary teams (MDTs) and this did not enable them to follow CPG recommendations. Two‐thirds of the participants surveyed describe the absence of centers of excellence in their country. In over half of the referral hospitals with expertise in managing PAS, there are no MDTs. One‐third of patients with intraoperative findings of PAS are managed by the team initially performing the surgery (without additional assistance).ConclusionThe care of patients with PAS in middle income countries frequently deviates from established CPG recommendations largely due to limitations in local resources and infrastructure. New practical guidelines and training programs designed for low resource settings are needed.
Complementary effects of information systems strategic assets and enabling roles of information systems on innovation performance of banking organizations
Authors: Obeng Asare Yaw , Opoku Samuel King , Obeng Ama Pokuah , Afriyie Emmanuel Oppong 
Published: March 2024

AbstractBanking organizations improve innovation performance by recognizing rare strategic assets and efficient use of information systems. This study explored the role of information systems in enhancing internal operational effectiveness and leveraging firm capabilities for strategic positions. Using a mixed‐method design, 11 participants in six banking organizations in Ghana were interviewed to gather data. The results indicate IT contribution, organizational learning, and IS‐related human are the most significant attributes that positively impact innovation performance. Corroborating qualitative and experimental results identified new products, improved operational processes, improved product functionalities, and innovation strategy as the key innovation performance factors. While the information systems strategic assets have minimal effect, the enabling role of information systems has a moderate impact on innovation performance. In contrast to either information systems strategic assets or the enabling role of information systems alone, the results show their complimentary effect on innovation performance is considerably higher.
Best linear and quadratic moments for spatial econometric models with an application to spatial interdependence patterns of employment growth in US counties
Authors: Jin Fei , Lee Lung‐fei , Yang Kai 
Published: March 2024

SummaryWe provide a novel analytic procedure to construct best linear and quadratic moments of the generalized method of moments estimation for a large class of cross‐sectional network and spatial econometric models. These moments generate an estimator that is asymptotically more efficient than the quasi‐maximum likelihood estimator when the disturbances follow a non‐normal and unknown distribution. We apply this procedure to a high‐order spatial autoregressive model with spatial errors, where the disturbances are heteroskedastic. Two normality tests of disturbances are developed. We apply the model to employment data in US counties, which demonstrates spatial interdependence patterns of regional employment growth.
Explainable artificial intelligence in the design of selective carbonic anhydrase I‐II inhibitors via molecular fingerprinting
Authors: Kırboğa Kevser Kübra , Işık Mesut 
Published: March 2024

AbstractInhibiting the enzymes carbonic anhydrase I (CA I) and carbonic anhydrase II (CA II) presents a potential avenue for addressing nervous system ailments such as glaucoma and Alzheimer's disease. Our study explored harnessing explainable artificial intelligence (XAI) to unveil the molecular traits inherent in CA I and CA II inhibitors. The PubChem molecular fingerprints of these inhibitors, sourced from the ChEMBL database, were subjected to detailed XAI analysis. The study encompassed training 10 regression models using IC50 values, and their efficacy was gauged using metrics including R2, RMSE, and time taken. The Decision Tree Regressor algorithm emerged as the optimal performer (R2: 0.93, RMSE: 0.43, time‐taken: 0.07). Furthermore, the PFI method unveiled key molecular features for CA I inhibitors, notably PubChemFP432 (C(O)N) and PubChemFP6978 (C(O)O). The SHAP analysis highlighted the significance of attributes like PubChemFP539 (C(O)NCC), PubChemFP601 (C(O)OCC), and PubChemFP432 (C(O)N) in CA I inhibitiotable n. Likewise, features for CA II inhibitors encompassed PubChemFP528(C(O)OCCN), PubChemFP791 (C(O)OCCC), PubChemFP696 (C(O)OCCCC), PubChemFP335 (C(O)NCCN), PubChemFP580 (C(O)NCCCN), and PubChemFP180 (C(O)NCCC), identified through SHAP analysis. The sulfonamide group (S), aromatic ring (A), and hydrogen bonding group (H) exert a substantial impact on CA I and CA II enzyme activities and IC50 values through the XAI approach. These insights into the CA I and CA II inhibitors are poised to guide future drug discovery efforts, serving as a beacon for innovative therapeutic interventions.
Establishment and methodological evaluation of a chemiluminescence assay for detection of anti‐envelope protein (E1, E2) antibodies in the serum of hepatitis C virus‐infected patients
Authors: Wang Ningning , Liu Qingqing , Che Feihu , Sun Qingyang , Wang Yue , Yang Chunli , Dai Yuzhu , Cheng Jun 
Published: March 2024

AbstractBackgroundTo establish a chemiluminescence method for detecting anti‐E1 and anti‐E2 antibodies in the serum of patients with hepatitis C virus (HCV) infection.MethodsThe microplate was coated with recombinant envelope proteins E1 and E2 by indirect method, respectively, and the kits for detecting anti‐E1 and anti‐E2 antibodies were prepared. The methodological indexes were evaluated.ResultsThe methodological indexes of the kits were as follows: precision test (the variation coefficient of anti‐E1 antibody 6.71%–8.95% for within run and 9.91%–12.16% for between run, the variation coefficient of anti‐E2 antibody 6.06%–8.44% for within run and 10.77%–13.98% for between run, respectively). The blank limit and detection limit were 1.18 RLIR and 3.16 RLIR for the anti‐E1 antibody, and 1.26 RLIR and 3.32 RLIR for the anti‐E2 antibody, respectively. The correlation coefficients (r) of anti‐E1 and anti‐E2 were 0.9963 and 0.9828, the analysis and measurement ranges (AMR) were 1.66–41.28 RLIR and 1.55–19.46 RLIR, and the average recovery was 96.4% and 93.7%, respectively. The rheumatoid factor and other positive serum samples had no interference or cross‐reaction to the test, and the kits were stable within 15 months. The positive rates of anti‐E1 and anti‐E2 antibodies in 45 patients with HCV infection were 35.6% (16/45) and 44.4% (20/45), respectively.ConclusionsThe kits for detecting anti‐E1 and anti‐E2 meet the requirements of methodology, and can be used in screening diagnosis, disease monitoring, prognosis evaluation, disease mechanism, and epidemiological studies of HCV infection. The HCV envelope proteins E1 and E2 have an immune response in HCV‐infected patients.
Hippocampal neurodegeneration induces transient endogenous regeneration and long‐term exhaustion of the neurogenic niche
Authors: Dey Jhilik , Chandra Sreyashi , Gupta Jalaj , Tripathi Prem Prakash 
Published: March 2024

AbstractThe hippocampal dentate gyrus, responds to diverse pathological stimuli through neurogenesis. This phenomenon, observed following brain injury or neurodegeneration, is postulated to contribute to neuronal repair and functional recovery, thereby presenting an avenue for endogenous neuronal restoration. This study investigated the extent of regenerative response in hippocampal neurogenesis by leveraging the well‐established kainic acid‐induced status epilepticus model in vivo. In our study, we observed the activation and proliferation of neuronal progenitors or neural stem cell (NSC) and their subsequent migration to the injury sites following the seizure. At the injury sites, new neurons (Tuj1+BrdU+ and NeuN+BrdU+) have been generated indicating regenerative and reparative roles of the progenitor cells. We further detected whether this transient neurogenic burst, which might be a response towards an attempt to repair the brain, is associated with persistent long‐term exhaustion of the dentate progenitor cells and impairment of adult neurogenesis marked by downregulation of Ki67, HoPX, and Sox2 with BrdU+ cell in the later part of life. Our studies suggest that the adult brain has the constitutive endogenous regenerative potential for brain repair to restore the damaged neurons, meanwhile, in the long term, it accelerates the depletion of the finite NSC pool in the hippocampal neurogenic niche by changing its proliferative and neurogenic capacity. A thorough understanding of the impact of modulating adult neurogenesis will eventually be required to design novel therapeutics to stimulate or assist brain repair while simultaneously preventing the adverse effects of early robust neurogenesis on the proliferative potential of endogenous neuronal progenitors.