bluuebunny/crossref_metadata_2025_split huggingface dataset:
Apoptotic effects of crude muscle extract from marine pufferfish Chelonodon patoca against human cancer cell lines
Authors: Sekar Niranjni , Valayapathi Rajasekar , Azhagudurai Aarthi , Durai Prabhu , Selvakumar Shiva Shakthi , Chinnasamy Arulvasu 
Published: February 2025

Abstract

Background
The global establishment of cancer registries has prompted a hunt for innovative medications that destroy cancer but not healthy cells. Researchers are currently searching marine environments for new anticancer drugs. Modern chemotherapy uses numerous compounds of aquatic origin. These substances exhibit cytotoxic characteristics through various methods, including DNA damage, apoptosis induction, and growth suppression of cancerous cells. The discovery and development of novel anticancer agents from abundant marine fish is generating increasing interest. The objective of this present study is to extract and assess the anti-proliferative effect of a crude muscle extract from marine pufferfish on human cancer cell lines.

Methods
The biochemical constituents, protein profile, and anticancer activities of the prepared crude muscle extract were analysed using standard methods on human cancer cell lines (HT-29, MDA-MB-231, A-549, and AGS).

Results
The biochemical contents, such as protein (7.19 ± 0.20 mg/mL), lipid (1.56 ± 0.14 mg/mL), and carbohydrate (1.19 ± 0.09 mg/mL), were quantitatively analysed. Native PAGE and SDS-PAGE qualitatively analysed the protein profiles, revealing distinct protein bands with molecular weights ranging from 220 to 14 kDa. The crude muscle extract was screened for its cytotoxicity (vero) and cell viability (HT-29, MDA-MB-231, A-549, and AGS) against human cancer cell lines by the MTT assay method. The nuclear morphological changes of the apoptotic cells were stained using propidium iodide, and the morphological changes associated with apoptosis were assessed using acridine orange/ethidium bromide (AO/EB) fluorescence staining. The intensity of the mitochondrial membrane potential of the treated cells was measured using the Rh-123 stain, and the results of the DNA fragmentation assay showed that the crude muscle extract-treated cells showed DNA damage, which is indicative of apoptosis.

Conclusions
This preliminary study supports that the crude muscle extract from milk spotted marine pufferfish, C. patoca has strong anticancer properties. This implies potential for the development of more effective anticancer drugs in future.

Graphical abstract
Assessment of myocardial work in sarcomere gene mutation carriers, healthy controls and overt nonobstructive hypertrophic cardiomyopathy
Authors: Pires Carla Marques , Joy George , Triantafyllou Miltiadis , Monteiro Ricardo Prista , Ferreira Ana , Savvatis Konstantinos , Lopes Luis Rocha 
Published: February 2025

Abstract

Background
Hypertrophic cardiomyopathy (HCM) is defined by unexplained hypertrophy and often characterized by diastolic and systolic dysfunction. HCM patients are known to have impaired left ventricular (LV) myocardial work (MW), a more load-independent parameter compared to global longitudinal strain (GLS). We hypothesized that impaired MW might occur in sarcomere mutation carriers without LV hypertrophy.

Methods and results
A single centre study with a case-control design. Patients with overt nonobstructive HCM and a causal sarcomere gene variant (n = 44), carriers (n = 51) and age and sex matched (to the carriers) healthy controls (n = 32) underwent a transthoracic echocardiogram including myocardial deformation analysis to calculate GLS and MW. Global work index (GWI) (1695 ± 332mmHg% vs. 1881.50 ± 490mmHg%, p = 0.001) and global constructive work (GCW) (2017.78 ± 323.05mmHg% vs. 2329.31 ± 485.44 mmHg%, p = 0.002) were lower in sarcomere mutation carriers compared to controls. LV ejection fraction and GLS were similar between these two groups. GWI (1209 ± 735mmHg% vs. 1695 ± 332mmhg%, p < 0.001), GCW (1456 ± 703mmHg% vs. 1993 ± 389mmHg%, p < 0.001), global wasted work (GWW) (117 ± 148mmHg% vs. 96 ± 69mmHg%, p = 0.006) and global work efficiency (GWE) (89 ± 7% vs. 95 ± 3%, p < 0.001)] were worse in overt non-obstructive HCM patients.

Conclusion
We show for the first time that MW indexes were significantly worse in sarcomere mutation carriers compared to controls, suggesting that MW is more sensitive to early changes than GLS and could have a significant role in the evaluation and follow-up of carriers.
Transdiagnostic use of 3,4-methylenedioxymethamphetamine-assisted therapy to treat obsessive–compulsive disorder
Authors: Saade Ziad , Keuroghlian Alex S. 
Published: February 2025

Summary
This article explores the potential of 3,4-methylenedioxymethamphetamine (MDMA)-assisted therapy to enhance exposure and response prevention in obsessive–compulsive disorder treatment. We discuss the mechanisms of MDMA, including fear extinction, psychological flexibility, and empathogenic effects that may improve adherence and efficacy, as well as highlighting important safety considerations for further research.
Impact of Technology on Quality of Thoracic Multidisciplinary Cancer Conferences
Authors: Ibekwe Opuruiche , Gaudioso Carmelo , Attwood Kristopher M. , Pokharel Saraswati , Roche Charles L. , Nwogu Chukwumere E. 
Published: February 2025

PURPOSE
Complex cancers require discussion at multidisciplinary cancer conferences (MCCs) to determine the best management. This study assessed the impact of a tumor board (TB)–specific information technology platform on the quality of information presented, case discussions, and care plans at thoracic MCCs.

METHODS
Between September 2020 and February 2022, using a before-after study design, we prospectively collected data through direct observation of thoracic MCCs at an academic cancer center. In addition, we reviewed medical records to assess the rate of change in care plans, compliance of all care plans with national guidelines, concordance of treatment received with MCC recommendations, and time from MCC presentation to treatment. Observational data were collected using a validated tool, Metric for the Observation of Decision-Making. We used SAS version 9.4 (SAS Institute Inc, Cary, NC) for statistical analyses.

RESULTS

We identified 151 and 166 thoracic cancer cases before and after implementation of the information technology platform, respectively. The overall quality of case presentation and discussion, represented by a mean composite score (summation of individual variables scored on a 1-5 scale, poor to excellent), increased from 56.8 to 82.0 (
P
< .001). This improvement was also observed across multiple subcomponents of the composite score all with
P
< .001. There was no statistically significant difference between the two cohorts in rate of change in care plans by the MCC, care plan compliance with national guidelines, and concordance of treatment received with MCC recommendations.

CONCLUSION
Technology improves the quality of information and discussion at TBs. However, this study did not demonstrate an impact on compliance with practice guidelines. Practitioners should explore the available TB technology platforms to optimize the conduct of MCCs in their respective institutions.
Abdominal tuberculosis with pancreatic head involvement mimicking pancreatic malignancy in a young man: A case report
Authors: Ma Lei , Wang Kun-Peng , Jin Chong 
Published: February 2025

BACKGROUND
Pancreatic tuberculosis (PTB) is a rare disease, even in immunocompetent hosts. Abdominal tuberculosis involving the pancreatic head and peripancreatic areas may simulate pancreatic head carcinoma.
CASE SUMMARY
We present the case of a 32-year-old man who was admitted to our hospital for intermittent epigastric pain and weight loss. A computed tomography scan and magnetic resonance imaging revealed a mass in the head of the pancreas. The lesion was initially diagnosed as pancreatic head carcinoma on abdominal imaging. Laparotomy confirmed the diagnosis of PTB and the patient received antituberculosis therapy.
CONCLUSION
The present case is reported to emphasize the importance of including PTB in the differential diagnosis of pancreatic lesions.
Analisis Vegetasi dan Luasan Mangrove di Mangunharjo, Kecamatan Tugu, Kota Semarang
Authors: Saputra Fadzil Eka Jaya , Taufiq-Spj Nur , Santosa Gunawan Widi 
Published: February 2025

Kelurahan Mangunharjo sering menjadi pusat kegiatan konservasi mangrove di Kota Semarang sejak awal tahun 2000-an. Tujuan dari adanya penelitian ini adalah untuk mengetahui kondisi ekosistem mangrove melalui analisa vegetasi dan perubahan luasan ekosistem mangrove di Kelurahan Mangunharjo. Pengambilan data vegetasi mangrove dilakukan di 4 stasiun dengan pengambilan data diameter batang, tutupan kanopi, substrat, dan parameter perairan. Perubahan luasan ekosistem mangrove menggunakan metode digitasi citra Google Earth 2002, 2007, 2012, 2017, dan 2021. Hasil penelitian menunjukkan bahwa vegetasi mangrove Kelurahan Mangunharjo ada kecenderungan meningkat dari tahun 2002 ke 2017 dan sedikit menurun di tahun 2021. Vegetasi didominasi oleh spesies Avicennia marina dengan kerapatan mangrove kategori pohon 2.258,4 ind/ha, persentase rata-rata tutupan kanopi 83,37% dengan kenaikan luasan mangrove sebesar 704% (2002-2021) yakni dari 7,5 ha pada tahun 2002 menjadi 60,3 ha pada tahun 2021. Mangunharjo has often been the center of conservation activities in Semarang City since the early 2000s. The purpose of this study was to determine the condition of the mangrove ecosystem through analysis of vegetation and changes of mangrove ecosystem area in Mangunharjo. Mangrove vegetation data was collected at 4 stations with data collection of stem diameter, canopy cover, substrate, and water parameters. Changes in the area of mangrove ecosystems by digitizing Google Earth images 2002, 2007, 2012, 2017, and 2021. The results showed that the mangrove vegetation in Mangunharjo Village tend to be increased during 2002 to 2017, and slightly decresed in 2021. The vegetation dominated by Avicennia marina species with a mangrove density of 2,258.4 ind/ha tree category, the average percentage of canopy cover is 83.37% with an increase in mangrove area of 704% in the period 2002-2021 i.e. 7.5 ha in 2002 to 60.3 ha in 2021.
Kelimpahan Megabenthos yang Berkaitan dengan Karakteristik Substrat di Ekosistem Padang Lamun Perairan Jepara
Authors: Febryana Syafitri Indah , Riniatsih Ita , Munasik Munasik 
Published: February 2025

Megabenthos menjadi salah satu kelompok biota laut yang berasosiasi dengan ekosistem padang lamun serta memiliki peran penting dalam pemanfaatan bahan organik di substrat perairan. Megabenthos hidup menetap di substrat dasar ekosistem padang lamun sehingga karakteristik substrat dan kandungan nutrient di dalamnya memengaruhi keberadaan dan kelimpahan megabenthos yang berasosiasi di ekosistem padang lamun. Penelitian ini bertujuan untuk mengetahui kelimpahan megabenthos di ekosistem padang lamun dan keterkaitannya terhadap karakteristik substrat dan parameter lingkungan pada Perairan Pantai Blebak, Pantai Semat, dan Pantai Ujung Piring, Jepara. Penelitian ini dilakukan dengan penentuan stasiun pengambilan data secara purposive sampling. Pengambilan data lamun dan megabenthos menggunakan metode line transect dan analisis data kelimpahan terhadap faktornya menggunakan Principal Component Analysis (PCA). Hasil pengamatan menunjukkan ditemukannya 27 spesies megabenthos dari filum Gastropoda, Bivalvia, Asteroidea, dan Holothuroidea pada ketiga lokasi pengamatan. Kelimpahan megabenthos Perairan Pantai Blebak dengan substrat dasar pasir halus, Pantai Semat dengan substrat pasir kasar berlumpur, dan Ujung Piring dengan substrat pasir sangat halus diperoleh dari yang terendah sebesar 0,10 ind/m2, 0,113 ind/m2, dan 0,13 ind/m2. Kelimpahan megabenthos dicirikan oleh faktor persentase tutupan lamun, karakteristik substrat, bahan organik, dan parameter fisika kimia perairan.
COLEC10: A potential tumor suppressor and prognostic biomarker in hepatocellular carcinoma through modulation of EMT and PI3K-AKT pathways
Authors: Ke Rui-Sheng , Dai Yun , Tu Yan-ling , Liu Zhao-Hui , Huang Kun-Zhai , Zhang Fu-Xing 
Published: February 2025

Abstract
Hepatocellular carcinoma (HCC) is a cancer with poor prognosis, underscoring the urgent need for enhanced detection and management. This study aimed to investigate the role of Collectin Subfamily Member 10 (COLEC10) in HCC, which was revealed to be associated with various diseases. Bioinformatics tools, including GEO, cBioPortal, and TCGA, were used to identify differentially expressed genes. The prognostic significance of COLEC10 was assessed in two patient cohorts, and its functional impact on Hep3B and SMMC7721 cells was evaluated through CCK-8 and Transwell assays. The underlying mechanisms of COLEC10 in HCC progression were explored using flow cytometry and western blot. COLEC10 was downregulated in HCC and associated with poorer overall survival and disease progression. The potential interaction of COLEC10, CCBE1, and FCN3 was predicted. COLEC10, CCBE1, and FCN3 were identified as prognostic indicators for HCC. Overexpression of COLEC10 inhibited the proliferation, migration, and invasion of HCC cells. COLEC10 overexpression induced G0/G1 cell cycle arrest and suppressed epithelial–mesenchymal transition (EMT), COLEC10 regulated protein expression in the Hedgehog pathway and phosphorylation of key proteins in the PI3K-AKT pathway. COLEC10 is an independent prognostic factor of HCC. COLEC10 regulates EMT, Hedgehog, and PI3K-AKT pathways, providing new ideas for targeted therapy of HCC.
Lifting subgroups of PSL2 to SL2 over local fields
Authors: Andrew Naomi , Conder Matthew J. , Markowitz Ari , Schillewaert Jeroen 
Published: February 2025

Abstract
Let 𝐾 be a non-archimedean local field.
We show that discrete subgroups without 2-torsion in

PSL
2

⁢

(
K
)

\mathrm{PSL}_{2}(K)

can always be lifted to

SL
2

⁢

(
K
)

\mathrm{SL}_{2}(K)

, and provide examples (when

char
⁡

(
K
)

≠
2

\operatorname{char}(K)\neq 2

) which cannot be lifted if either of these conditions is removed.
We also briefly discuss lifting representations of groups into

PSL
2

⁢

(
K
)

\mathrm{PSL}_{2}(K)

to

SL
2

⁢

(
K
)

\mathrm{SL}_{2}(K)

.
Würzburger Thesen zur Angewandten Sprachwissenschaft
Authors: Schulz Matthias , Schwebler Miriam 
Published: February 2025

Abstract
Applied Linguistics is Linguistics Plus: Compared to non-applied linguistic research, applied linguistics is characterized by a socially cooperative approach that focuses on linguistic and language-related problems and problem areas, a solution-oriented research design, networking with the outside world, and special application skills of the researchers.This is the conclusion of the conference “Applied and Applicable Linguistics – A Position Determination,” held in Würzburg in December 2023. The conference responded to an observation that can be made from a review of theoretical discussions of applied linguistics, but also from observing applied linguistic practice, for example at the conferences of the Gesellschaft für Angewandte Linguistik e. V.: Despite various attempts to define it, there are currently very different views of what applied linguistics is, what specific features characterise it and how it can be delineated from other areas or methodologies of linguistics. This normally causes no issue when, for example, negotiating different positions and a broad research discourse that promotes science – in practice, however, it makes it more difficult for applied linguistics to be perceived and recognised as a discipline in the research landscape and in society. Colleagues working in higher education as well as other sectors often point out the negative consequences for interdisciplinary cooperation, the acquisition of funding or the recruitment of early career researchers. The aim of the conference was therefore to discuss and clarify how applied linguistics is currently defined in German-speaking research practice and, at the same time, to outline a vision for the future ideally sparking further discussion. The focus was not on narrowing discourse, but rather on promoting discourse. The results of the conference are presented here in the form of theses, individually commented on by conference participants. They are intended as a starting point for further programmatic discussion.
The Analytical Survey Method; A valuable tool for evaluation and provision of efficient and effective Occupational Therapy services for a patient/client Population.
Authors: Van der Reyden Dain , Wilson Sarah , White Beth 
Published: February 2025

This paper describes the effective integration and application of a hybrid analytical survey method in order to obtain a substantive profile and resource information about a patient/client population. Integrating the Vona du Toit Model of Creative Ability, the survey is used as a primary tool for planning intervention, evaluation and planning in occupational therapy practice. The process has been used extensively in action research in practices throughout South Africa and the United Kingdom and results have shown that it allows for a more targeted and efficient provision of intervention and treatment. This paper briefly describes the development of the Analytical Survey Method and details how the procedure is implemented using the example of its application in a United Kingdom healthcare facility. Its contemporary relevance is highlighted in the context of healthcare challenges and the need to demonstrate efficient and effective occupational therapy procision.occupational therapy provision.
Key words: Occupational Therapy; analytical survey; analytical survey method: OT service planning and evaluation; Vona du Toit Model of Creative Ability; action research: service efficiency
In vivo antibacterial activity and biochemical effects of methanol extract of Annona muricata leaves against multidrug- resistant Salmonella Typhimurium in Wistar rats
Authors: Fondeh Brenda Mazongue , Awah Laupy Anne , Ndip Rita Ayuk , Kibu Odette Dzemo , Ngemenya Moses Njutain 
Published: February 2025

Background: The increasing antibiotic resistance and paucity of new antibiotics has contributed greatly to the high morbidity and mortality of salmonellosis necessitating the search for alternative treatments. Annona muricata has shown promising activity against multidrug-resistant (MDR) Salmonella in vitro but in vivo studies are rare. This study evaluated the activity of A. muricata, against MDR Salmonella Typhimurium in vivo.
Methods: The minimum inhibitory concentration (MIC) of a methanol crude extract of A. muricata was determined by micro-dilution assay against a characterized MDR clinical S. Typhimurium strain. Wistar rats infected with 3 x 108 CFUs/mL of the MDR strain were treated with 50-200 mg/kg body weight of extract for 10 days. Faecal load of S. Typhimurium colonies was determined by the direct plate count technique on days 1, 5 and 10. Animals were sacrificed and blood was collected for biochemical analyses. Data were analysed using GraphPad Prism Software.
Results: The S. Typhimurium strain was multidrug-resistant and the extract recorded a MIC of 2 mg/ml The extract produced a significant (p<0.001) dose-dependent reduction in Salmonella colonies in faeces of treated rats with a 100% inhibition recorded at 200 mg/kg body weight on day 10. Liver and renal function tests did not indicate any abnormalities (p<0.05).
Conclusions: This is the first report of in vivo activity of A. muricata leaves against multidrug-resistant Salmonella. The high activity and lack of adverse toxicity supports it use in traditional medicine and hence is a potential treatment for resistant Salmonella infections.
Liver biomarkers of silymarin milk thistle on paracetamol induced liver toxicity in adult albino rats (Rattus norvegicus)
Authors: Ngetich Davis Kiprono , Korir Scolastica 
Published: February 2025

Background: Paracetamol is the most common analgesic that is readily available with or without prescription over the counter and its toxicity is due to misuse or overdose associated with liver damage. Silymarin milk thistle is an edible herb believed to have antioxidant, scavenging and regulation of glutathione contents within the cell. Aim of the study was to determine the effects of different doses of silymarin milk thistle on liver biomarkers of paracetamol induced liver toxicity among albino rats.
Methods: Twenty-four adult albino rats were randomly grouped into four groups each group consisting of six animals. Paracetamol-only group given high dose paracetamol-750 mg/kbwt and control group received water and food only. The experimental group, were divided into three subgroups with all receiving high dose paracetamol for five days and thereafter given varying doses of silymarin milk thistle. SIL-G1 given 200 mg/kbwt of silymarin milk thistle, SIL-G2 given 400 mg/kbwt of silymarin milk thistle and SIL-G3 was given 600 mg/kbwt of silymarin milk thistle. All the animals were fed with standard rodent foods and water ad libitum. After completion of 24 hours of the last drug administration, they were humanely sacrificed, serum biochemical analysis of liver biomarkers (ALP, ALT and AST) was determined.
Results: High dose of paracetamol induced liver toxicity. Upon administration of high dose of silymarin milk thistle, there was restoration of ALP, AST and ALP of all these biomarkers in relation to the control group.
Conclusions: These findings showed that high dose (600 mg/kbwt) of silymarin milk thistle was found to have restorative effects and normalized liver biomarkers of ALP, AST and ALT.
Experience in the Prevention and Avoidance of Corruption in the Sphere of State and Municipal Procurement in the Sverdlovsk Region
Authors: Shiraliev I. S. 
Published: February 2025

Aim. To analyze the experience of prevention and avoidance of corruption offenses in the sphere of state and municipal procurement in the Russian Federation on the example of the Sverdlovsk Oblast.Methodology. Analytical and descriptive methods were used to collect information for the study. In particular, data on purchases organized by municipal authorities of the Sverdlovsk Oblast, as well as certificates of income of civil servants were analyzed. The experience of prevention of corruption offenses in the Sverdlovsk Oblast was also described: anti-corruption education, monitoring of procurement documents, connection to the Poseidon anti-corruption information system.Results. Empirical confirmation of the hypothesis about the need for comprehensive work to prevent corruption in public authorities was obtained on the material of the Sverdlovsk Oblast.Research implications. The results obtained can be used in planning practical actions to counteract corruption in the regions of the Russian Federation and in further theoretical research of the issue.