bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunization of rabbits with DNase I produces polyclonal antibodies with DNase and RNase activities
Authors: Krasnorutskii Michael A. , Buneva Valentina N. , Nevinsky Georgy A. 
Published: April 2008

AbstractImmunization of rabbits with DNase I leads to the production of antiidiotypic Abs with DNase activity. It is not known at present whether antiidiotypic Abs against DNA‐hydrolyzing enzymes can possess RNase activity. Here we show that immunization of healthy rabbits with bovine DNase I produces IgGs with intrinsic DNase and RNase activities. Electrophoretically and immunologically homogeneous polyclonal IgGs were obtained by sequential chromato‐graphy of the immune sera on Protein A‐Sepharose and gel filtration. Affinity chromatography on DNA cellulose using elution of Abs with different concentrations of NaCl and an acidic buffer separated catalytic IgGs into four Ab subfractions, three of which demonstrated only DNase activity while one subfraction hydrolyzed RNA faster than DNA. The serum of patients with many different autoimmune (AI) diseases contains small fractions of antibodies (Abs) interacting with immobilized DNA, which possess both DNase and RNase activities. Our data suggest that a fraction of abzymes from AI patients hydrolyzing both DNA and RNA can contain a subfraction of Abs against DNase I. Copyright © 2008 John Wiley & Sons, Ltd.
A fully validated isotope dilution HPLC‐MS/MS method for the simultaneous determination of succinylcholine and succinylmonocholine in serum and urine samples
Authors: Kuepper Uta , Musshoff Frank , Madea Burkhard 
Published: April 2008

AbstractA high performance liquid chromatography‐electrospray ionization‐tandem mass spectrometry (HPLC‐ESI‐MS/MS) method for the simultaneous detection of succinylcholine (SUX) and its metabolite succinylmonocholine (SMC) in serum and urine is presented. For internal standardization using isotope dilution, the deuterated compounds SUX‐d18 and SMC‐d3 were employed. Full validation was performed according to international guidelines. Solid‐phase extraction (SPE) of acidified samples was accomplished using Strata‐X polymeric reversed phase cartridges together with heptafluorobutyric acid (HFBA) as ion‐pairing reagent. Separation was achieved within 13 min on a Phenomenex Synergi Hydro RP C18 column (4 µm, 150 × 2 mm) using a gradient of 5 mM ammonium formate buffer pH 3.5 and acetonitrile.To ensure the method's applicability in forensic as well as clinical toxicology, the specific demands of both research fields were taken into account, and the method was thus validated for a low and high concentration range. For both serum and urine as sample matrix, the validation revealed good intraday and interday precisions, consistently ranging below 15% for the lowest and below 10% for elevated concentrations. Accuracy was likewise good and never exceeded 10%. Extraction recovery was excellent, ranging between 88.1 and 103.9% for SUX and SMC in both tested matrices. Matrix effects were significant, the otherwise optimized extraction and detection methods, however, allowed for a very satisfactory sensitivity of the described method: For serum, the limits of detection and quantitation were determined to be 1.9 and 6.0 ng/ml for SUX, as well as 2.5 and 8.6 ng/ml for SMC, respectively; for urine, the corresponding values were established to be 1.4 and 4.0 ng/ml (SUX), as well as 1.5 and 4.9 ng/ml (SMC).The presented method was successfully applied to authentic samples of two forensic cases investigated in the institute of forensic medicine in Bonn, allowing the diagnosis of SUX intoxications. Copyright © 2008 John Wiley & Sons, Ltd.
Hydrogen bubble‐assisted syntheses of polypyrrole micro/nanostructures using electrochemistry: structural and physical property characterization
Authors: Gupta Sanju 
Published: April 2008

AbstractPolypyrrole (PPy) in the family of π‐conjugated polymers is a potential candidate for microscopic actuators, electrochromic devices, batteries, and drug delivery systems. We report the controlled syntheses of PPy micro/nanocontainers through electrochemical generation of H2 bubbles from a surfactant cum electrolyte β‐NSA (β‐naphthalene sulfonic acid) stabilized on the working electrode, followed by electrochemical polymerization of monomer (pyrrole) around the wall of the micelles serving as template. It is noticed that the density, shape, caliber, and thickness of self‐assembled PPy micro/nanocontainers can be regulated by electrochemical potential window for H2 bubbles and number of cyclic voltammetric scans for the electropolymerization of pyrrole. Sodium chlorate and perchlorate are used to synthesize conventional PPy films. By changing the dopant structure, one can control the microscopic structure and physical properties, which are analyzed using multiple microscopic techniques and X‐ray diffraction (XRD), infrared, vis Raman and X‐ray photoelectron spectroscopic analytical tools. In light of this information, an exhaustive description of the material is attained. Experimental results show that the films consist of spherical/globular surface morphology and cup‐/bowl‐like structures, which becomes lantern‐like with increasing cyclic voltammetry scans. The bowl diameter and caliber and room temperature conductivities range 0.05–60 µm, 5–50 µm and 2 × 10−2–20 S cm−1, respectively. Raman spectroscopy (RS) helps to identify the oxidized PPy; polaron and bipolaron states and thus a detailed molecular structure. The results also showed that the relative conjugation length increases with decreasing size of the morphological feature. Moreover, these studies enhanced the understanding of bubble nucleation at the solid–electrolyte interface. Copyright © 2008 John Wiley & Sons, Ltd.
Raman spectroscopy of advanced glycation end products (AGEs), possible markers for progressive retinal dysfunction
Authors: Pawlak Anna M. , Beattie J. Renwick , Glenn Josephine V. , Stitt Alan W. , McGarvey John J. 
Published: April 2008

AbstractRaman microscopy is used to investigate the spectral features of selected compounds known to be involved in the development of the eye disease age‐related macular degeneration (AMD). Diagnostic features were identified in synthetic samples of these compounds and in a biological matrix. The study demonstrates the potential of Raman microscopy for the development of diagnostic markers of the onset of AMD. Copyright © 2008 John Wiley & Sons, Ltd.
Multiple primary malignancies in patients with sporadic pancreatic endocrine tumors
Authors: Fendrich Volker , Waldmann Jens , Bartsch Detlef K. , Schlosser Katja , Rothmund Matthias , Gerdes Berthold 
Published: April 2008

AbstractBackgroundTo investigate the appearance of multiple primary malignancies in patients with sporadic neuroendocrine pancreatic tumors (NEPTs).MethodsOne hundred forty‐five patients with NEPTs were treated at the Department of Surgery, Philipps‐University Marburg. Multiple primary malignancies included tumors that were not considered to be a metastasis, invasion, or recurrence of NEPTs. Data on sex, age at diagnosis of cancer, follow‐up time after diagnosis, and death rate were collected.ResultsOf 115 patients with sporadic NEPTs, 15 (13.0%) patients were identified with at least one malignant tumor, other than a NEPT. The median age at diagnosis of the associated tumor(s) was 57 years (range, 10–72 years). Two of the 15 patients had insulinomas, 5 had gastrinomas and 8 had non‐functioning NEPTs, respectively. The risk of developing multiple cancers was the highest for patients with gastrinoma (21.7%), followed by patients with non‐functioning NFPTs (20.5%).ConclusionsIn patients with NEPTs multiple primary malignancies are found more frequently than in the general population. The etiology of the increased risk of other primaries is not clearly defined, but it may be the result of accumulated growth stimulation by the secreted hormones or a genetic alteration that leads to tumorogenesis in these patients. J. Surg. Oncol. 2008;97:592–595. © 2008 Wiley‐Liss, Inc.
Improved local control by surgery and paclitaxel‐based chemoradiation for esophageal squamous cell carcinoma: Results of a retrospective non‐randomized study
Authors: Hsu Feng‐Ming , Lin Chia‐Chi , Lee Jang‐Ming , Chang Yih‐Leong , Hsu Chih‐Hung , Tsai Yu‐Chieh , Lee Yung‐Chie , Cheng Jason Chia‐Hsien 
Published: April 2008

AbstractBackground and ObjectivesTo investigate the impact of using paclitaxel in chemoradiation on locally advanced esophageal squamous cell carcinoma (SCC) treated with or without surgery.MethodsOf 127 patients with AJCC stages II–III esophageal SCC undergoing definitive chemoradiation (DefCRT, n = 44) or neoadjuvant chemoradiation plus surgery (NeoCRT + S, n = 83), 57 received chemotherapy with paclitaxel and cisplatin (TP), and 70 received 5‐fluorouracil and cisplatin (PF). Three‐year local progression‐free survival (LPFS), distant metastasis‐free survival, overall survival, and prognostic factors were retrospectively analyzed.ResultsThe median survival was 30 months. Pathological complete response rate was 41% and 27% with TP and PF, respectively (P = 0.19). NeoCRT + S achieved significantly higher LPFS than DefCRT (71% vs. 39%, P < 0.001). Patients receiving TP had significantly higher LPFS than PF (74% vs. 48%, P = 0.04). Local control was similar between DefCRT with TP and NeoCRT + S. Distant metastasis‐free survival and overall survival were not different between treatment modalities or chemotherapy regimens. In multivariate analysis, surgery (HR 0.30, P < 0.001), TP regimen (HR 0.38, P = 0.007), and mediastinal lymphadenopathy (HR 2.37, P = 0.008) were independent factors for LPFS.ConclusionsBoth surgery and the use of paclitaxel‐based chemoradiation may improve local disease control. Future randomized trials should integrate paclitaxel into definitive chemoradiation. J. Surg. Oncol. 2008;98:34–41. © 2008 Wiley‐Liss, Inc.
Europe Replaces Asia as the Growth Locomotive
Authors: Mayer Arnold 
Published: April 2008

AbstractAfter the record of 27 % growth in 2006, the global market for laser material processing systems saw a moderate growth of 5 % in 2007. This matches with the long term growth trend, taking into account economic cycles and currency fluctuations. Optech Consulting expects market growth at an average rate of 8 % to 9 %. Still, established applications, such as cutting, welding, and marking, are not yet saturated while new applications contribute to the growth potential.
Fabrication of Super Hydrophobic Surfaces by fs Laser Pulses
Authors: Groenendijk Max 
Published: April 2008

AbstractThe chair of Applied Laser Technology of the University of Twente, The Netherlands, is performing research into applications of ultrashort pulsed lasers for micromachining. In a recent project, PhD student Max Groenendijk developed a method for the production of super water repellant surfaces by injection molding. The core of this technique is the structuring of mold surfaces by micro and nanometer sized textures, using a femtosecondpulsed laser.
Cardiac sympathetic denervation correlates with clinical and pathologic stages of Parkinson's disease
Authors: Fujishiro Hiroshige , Frigerio Roberta , Burnett Melinda , Klos Kevin J. , Josephs Keith A. , DelleDonne Anthony , Parisi Joseph E. , Ahlskog J. Eric , Dickson Dennis W. 
Published: April 2008

AbstractAttention has been drawn to cardiac sympathetic denervation in Parkinson's disease (PD) based on clinical studies using [123I] metaiodobenzylguanidine scintigraphy; however, the histologic correlates and time course of cardiac sympathetic denervation are poorly understood. To address these issues, we used tyrosine hydroxylase (TH) immunohistochemistry to detect cardiac sympathetic nerve fibers in the epicardium of 4 normal controls, 11 cases with incidental Lewy bodies (iLBs), and 14 cases of PD. Cardiac sympathetic innervation was significantly less in PD than in normal controls and cases with iLBs (P < 0.05). There was also a decrease in TH‐immunoreactive fibers in iLB cases compared to normal controls (P < 0.01). TH‐immunoreactive fibers correlated with the PD stage (r = −0.75, P < 0.001), as well as with Hoehn & Yahr clinical stage (r = −0.61, P < 0.001), and disease duration (r = −0.63, P < 0.001). Immunohistochemistry for α‐synuclein showed neurites in epicardium in PD and iLB cases, but not in normal controls. The density of α‐synuclein neurites correlated with Braak PD stage (r = 0.38, P < 0.05), Hoehn & Yahr clinical stage (r = 0.44, P < 0.05), and disease duration (r = 0.42, P < 0.05). This study demonstrates that cardiac sympathetic degeneration and α‐synuclein pathology is present in presymptomatic phase of PD, and that both increase with disease duration and severity. © 2008 Movement Disorder Society.
Efficacy of long‐term continuous subcutaneous apomorphine infusion in advanced Parkinson's disease with motor fluctuations: A multicenter study
Authors: García Ruiz Pedro J. , Sesar Ignacio Ángel , Ares Pensado Begoña , Castro García Alfonso , Alonso Frech Fernando , Álvarez López Mercedes , Arbelo González José , Baiges Octavio Joan , Burguera Hernández Juan Andrés , Calopa Garriga Matilde , Campos Blanco Dulce , Castaño García Belén , Carballo Cordero Manuel , Chacón Peña José , Espino Ibáñez Anna , Gorospe Onisalde Aránzazu , Giménez‐Roldán Santiago , Granés Ibáñez Pilar , Hernández Vara Jorge , Ibáñez Alonso Ramón , Jiménez Jiménez Félix Javier , Krupinski Jerzy , Kulisevsky Bojarsky Jaime , Legarda Ramírez Inés , Lezcano García Elena , Martínez‐Castrillo Juan Carlos , Mateo González Dolores , Miquel Rodríguez Francesc , Mir Pablo , Muñoz Fargas Elena , Obeso Inchausti José , Olivares Romero Jesús , Olivé Plana José , Otermin Vallejo Pilar , Pascual Sedano Berta , Pérez de Colosía Rama Víctor , Pérez López‐Fraile Isabel , Planas Comes Albert , Puente Periz Víctor , Rodríguez Oroz María Cruz , Sevillano García Dolores , Solís Pérez Pilar , Suárez Muñoz José , Vaamonde Gamo Julia , Valero Merino Caridad , Valldeoriola Serra Francesc , Velázquez Pérez José Miguel , Yáñez Baña Rosa , Zamarbide Capdepon Ivana 
Published: April 2008

AbstractContinuous subcutaneous apomorphine infusion (CSAI) is, at present, an alternative option for advanced Parkinson's disease (PD) with motor fluctuations. We studied the evolution of patients with PD and severe motor fluctuations long‐term treated with CSAI. We reviewed data from 82 patients with PD (mean age, 67 ± 11.07; disease duration, 14.39 ± 5.7 years) and severe motor fluctuations referred to 35 tertiary hospitals in Spain. These patients were long‐term treated (for at least 3 months) with CSAI and tolerated the procedure without serious side effects. We compared the baseline data of these 82 patients (before CSAI) with those obtained from the last follow‐up visit of each patient. The mean follow‐up of CSAI was 19.93 ± 16.3 months. Mean daily dose of CSAI was 72.00 ± 21.38 mg run over 14.05 ± 1.81 hours. We found a statistically significant reduction in off‐hours, according to self‐scoring diaries (6.64 ± 3.09 vs. 1.36 ± 1.42 hours/day, P < 0.0001), total and motor UPDRS scores (P < 0.0001), dyskinesia severity (P < 0.0006), and equivalent dose of antiparkinsonian therapy (1,405 ± 536.7 vs. 800.1 ± 472.9 mg of levodopa equivalent units P < 0.0001). CSAI is an effective option for patients with PD and severe fluctuations, poorly controlled by conventional oral drug treatment. © 2008 Movement Disorder Society.
An approximation result for free discontinuity functionals by means of non‐local energies
Authors: Lussardi Luca 
Published: April 2008

AbstractWe approximate, in the sense of Γ‐convergence, free discontinuity functionals with linear growth by a sequence of non‐local integral functionals depending on the average of the gradient on small balls. The result extends to a higher dimension what is already proved in (Ann. Mat. Pura Appl. 2007; 186(4): 722–744), where there is the proof of the general one‐dimensional case, and in (ESAIM Control Optim. Calc. Var. 2007; 13(1):135–162), where the n‐dimensional case with ϕ=Id is treated. Moreover, we investigate whether it is possible to approximate a given free discontinuity functional by means of non‐local energies. Copyright © 2008 John Wiley & Sons, Ltd.
Explicit representations of the regular solutions to the time‐harmonic Maxwell equations combined with the radial symmetric Euler operator
Authors: Cação Isabel , Constales Denis , Kraußhar Rolf Sören 
Published: April 2008

AbstractIn this paper we consider a generalization of the classical time‐harmonic Maxwell equations, which as an additional feature includes a radial symmetric perturbation in the form of the Euler operator $E:={\textstyle\sum\nolimits_{i}}\,x_i {\partial }/{\partial x_i}$.We show how one can apply hypercomplex analysis methods to solve the partial differential equation (PDE) system [D–λ–αE]f=0, where D is the Euclidean Dirac operator and where λ and α are arbitrary non‐zero complex numbers. When α is a positive real number, the vector‐valued solutions to this PDE system provide us precisely with the solutions to the time‐harmonic Maxwell equations on the sphere of radius 1/α. We give a fully explicit description of the regular solutions around the origin for general complex α, λ ∈ ℂ\{0} in terms of hypergeometric functions and special homogeneous monogenic polynomials. We also discuss the limit cases λ → 0 and α → 0. In the latter one, we actually recognize the classical time‐harmonic solutions to the Maxwell equations in the Euclidean space. Copyright © 2008 John Wiley & Sons, Ltd.
Studies on inclusion complexes of calix[4]arenes capped by diamide bridges with small organic molecules
Authors: Balázs Barbara , Grün Alajos , Bitter István , Tóth Gábor 
Published: April 2008

AbstractThe inclusion of small neutral organic guests (acetonitrile, toluene, pyrazine, butylamine, nitromethane) by cyclic calix[4]arene diamide receptors was studied by 1H NMR spectroscopy. The binding constants determined by 1H NMR titration, and the results obtained by T1 relaxation measurements and DOSY confirm the importance of the acidity of the CH bond of the guests and highlight the role of steric interactions including conformational properties of the receptors in the recognition process. Copyright © 2008 John Wiley & Sons, Ltd.
High‐resolution NMR spectra of n‐alkanes penetrating into carbon fibers and of protons in carbon fibers
Authors: Yamazaki Yuya , Kobayashi Nobuaki , Nikki Kunio , Kuwahara Daisuke 
Published: April 2008

AbstractWe present a simple NMR method for microscopically exploring the local environment in carbon fibers. The method utilizes n‐alkanes as probe molecules, where the n‐alkanes penetrate carbon fibers of interest. The high‐resolution 1H NMR spectra for a mixture of a carbon fiber and n‐alkanes acquired by this method show a shift of the resonance line, which is due to the local structure of the fiber. The utility of this method is discussed on the basis of the 1H NMR spectra obtained. In addition, the 1H distribution and the local motion in the structure of the carbon fiber are revealed in view of the 1H NMR spectra. Copyright © 2008 John Wiley & Sons, Ltd.
Adjudin‐mediated germ cell depletion alters the anti‐oxidant status of adult rat testis
Authors: Sarkar Oli , Mathur P.P. 
Published: April 2008

AbstractSuccessful spermatogenesis is dependent on the proper attachment of developing germ cells to Sertoli cells. Manipulation of these interactions by drugs like Adjudin can hamper the development of germ cells and lead to conditions of temporary infertility. Although studies have shown the contraceptive potential of Adjudin, much is not known about its action in the testis. In this study, we sought to investigate the effect of Adjudin on the oxidative status of mammalian testis. Adult male rats were administered with a single dose of Adjudin (50 mg/kg body weight) by oral gavage and were killed at 1, 2, 4, 7, 15, or 30 days of treatment. Adjudin caused a significant increase in the production of hydrogen peroxide and lipid peroxidation from 4 to 7 days after treatment. There was a significant decrease in the activities of anti‐oxidant enzymes superoxide dismutase, catalase, glutathione peroxidase and glutathione S‐transferase from 4 to 7 days after treatment with Adjudin. However, the state of oxidative stress was less pronounced from 15 to 30 days after Adjudin treatment. The level of androgen binding protein (ABP) remained unchanged following Adjudin treatment. These results show that there is an induction of oxidative stress accompanying adherens junction restructuring which suggests a role for reactive oxygen species in the regulation of these testicular junctions. However, transient elevation in reactive oxygen species levels did not affect androgen transport. Mol. Reprod. Dev. 76: 31–37, 2009. © 2008 Wiley‐Liss, Inc.
Transgenic sheep designed for transplantation studies
Authors: Ritchie William A. , King Tim , Neil Claire , Carlisle Ailsa J. , Lillico Simon , McLachlan Gerry , Whitelaw C. Bruce A. 
Published: April 2008

AbstractLentiviral vectors have recently emerged as an efficient method of transgene delivery to the germline of animals. We now demonstrate that combining this efficiency with embryo splitting procedures enables the production of monozygotic twins, one of which is transgenic. We propose that this approach can be used to generate animals in which cell or tissue transplantation can be achieved without the use of immunosuppressive regimes. Mol. Reprod. Dev. 76: 61–64, 2009. © 2008 Wiley‐Liss, Inc.
Complementary cycles of restarted GMRES
Authors: Zhong Baojiang , Morgan Ronald B. 
Published: April 2008

AbstractRestarted GMRES is one of the most popular methods for solving large nonsymmetric linear systems. It is generally thought that the information of previous GMRES cycles is lost at the time of a restart; therefore, each cycle contributes to the global convergence individually. However, this is not the full story. In this paper, we shed light on the relationship between different GMRES cycles. It is shown that successive GMRES cycles can complement one another harmoniously. These groups of cycles, called complementary cycles, are defined and studied. Copyright © 2008 John Wiley & Sons, Ltd.
A handy proof of Gay's theorem
Authors: Adib Majid 
Published: April 2008

AbstractIn 1979, Gay proved that Broyden's methods, when used for n‐square linear systems, terminate in at most 2n iterations (SIAM J. Numer. Anal. 1979; 16:623–630). Also, the ABS methods were introduced in 1984 (Numer. Math. 1984; 45:1361–1376). In this paper we show another (handy) proof of Gay's theorem by these algorithms. Copyright © 2008 John Wiley & Sons, Ltd.
Data assimilation and inverse problem for fluid traffic flow models and algorithms
Authors: Jaisson P. , DeVuyst F. 
Published: April 2008

AbstractThis article deals with traffic data assimilation and algorithms that are able to predict the traffic flow on a road section. The traffic flow is modellized by the Aw–Rascle hyperbolic system. We have to minimize a functional whose optimization variables are initial condition. We use the Roe method to compute the solution to the traffic flow modelling system. Then we compute the gradient of the functional by an adjoint method. This gradient will be used to optimize the functional. Copyright © 2008 John Wiley & Sons, Ltd.