bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recessive resistance against beet chlorosis virus is conferred by the eukaryotic translation initiation factor (iso)4E in Beta vulgaris
Authors: Rollwage Lukas , Van Houtte Hilde , Hossain Roxana , Wynant Niels , Willems Glenda , Varrelmann Mark 
Published: March 2024

SummaryEukaryotic translation initiation factors (eIFs) are important for mRNA translation but also pivotal for plant‐virus interaction. Most of these plant‐virus interactions were found between plant eIFs and the viral protein genome‐linked (VPg) of potyviruses. In case of lost interaction due to mutation or deletion of eIFs, the viral translation and subsequent replication within its host is negatively affected, resulting in a recessive resistance. Here we report the identification of the Beta vulgaris Bv‐eIF(iso)4E as a susceptibility factor towards the VPg‐carrying beet chlorosis virus (genus Polerovirus). Using yeast two‐hybrid and bimolecular fluorescence complementation assays, the physical interaction between Bv‐eIF(iso)4E and the putative BChV‐VPg was detected, while the VPg of the closely related beet mild yellowing virus (BMYV) was found to interact with the two isoforms Bv‐eIF4E and Bv‐eIF(iso)4E. These VPg‐eIF interactions within the polerovirus‐beet pathosystem were demonstrated to be highly specific, as single mutations within the predicted cap‐binding pocket of Bv‐eIF(iso)4E resulted in a loss of interaction. To investigate the suitability of eIFs as a resistance resource against beet infecting poleroviruses, B. vulgaris plants were genome edited by CRISPR/Cas9 resulting in knockouts of different eIFs. A simultaneous knockout of the identified BMYV‐interaction partners Bv‐eIF4E and Bv‐eIF(iso)4E was not achieved, but Bv‐eIF(iso)4EKO plants showed a significantly lowered BChV accumulation and decrease in infection rate from 100% to 28.86%, while no influence on BMYV accumulation was observed. Still, these observations support that eIFs are promising candidate genes for polerovirus resistance breeding in sugar beet.
Half of the 18O enrichment of leaf sucrose is conserved in leaf cellulose of a C3 grass across atmospheric humidity and CO2 levels
Authors: Cabrera Juan C. Baca , Hirl Regina T. , Schäufele Rudi , Zhu Jianjun , Liu Hai Tao , Gong Xiao Ying , Ogée Jérôme , Schnyder Hans 
Published: March 2024

AbstractThe 18O enrichment (Δ18O) of cellulose (Δ18OCel) is recognized as a unique archive of past climate and plant function. However, there is still uncertainty regarding the proportion of oxygen in cellulose (pex) that exchanges post‐photosynthetically with medium water of cellulose synthesis. Particularly, recent research with C3 grasses demonstrated that the Δ18O of leaf sucrose (Δ18OSuc, the parent substrate for cellulose synthesis) can be much higher than predicted from daytime Δ18O of leaf water (Δ18OLW), which could alter conclusions on photosynthetic versus post‐photosynthetic effects on Δ18OCel via pex. Here, we assessed pex in leaves of perennial ryegrass (Lolium perenne) grown at different atmospheric relative humidity (RH) and CO2 levels, by determinations of Δ18OCel in leaves, Δ18OLGDZW (the Δ18O of water in the leaf growth‐and‐differentiation zone) and both Δ18OSuc and Δ18OLW (adjusted for εbio, the biosynthetic fractionation between water and carbohydrates) as alternative proxies for the substrate for cellulose synthesis. Δ18OLGDZW was always close to irrigation water, and pex was similar (0.53 ± 0.02 SE) across environments when determinations were based on Δ18OSuc. Conversely, pex was erroneously and variably underestimated (range 0.02–0.44) when based on Δ18OLW. The photosynthetic signal fraction in Δ18OCel is much more constant than hitherto assumed, encouraging leaf physiological reconstructions.
Urban Colombian youths' perspectives on the armed conflict and possibilities for ways forward
Authors: Pareja Conto Laura , Velez Gabriel , Restrepo Angelica , Recchia Holly , Posada‐Gilede Roberto , Wainryb Cecilia 
Published: March 2024

AbstractIn the aftermath of intractable armed conflict, adolescents can play a critical role in the process of reconciliation and the construction of a culture of peace. The meanings they construct about these processes are a core aspect of their engagement (or lack thereof) in peace‐building initiatives. This study sought to document adolescents' perspectives on the Colombian armed conflict and the 2016 peace treaty, as well as their preferred resolutions to move forward after decades of harm and division. We interviewed 77 adolescents (39 girls, 38 boys) ranging from ages 14 to 19 years (M = 16.49, SD = .95) from two public schools in Bogotá, Colombia. We asked youth open‐ended questions about the history of the armed conflict, their position toward the peace accord, and their proposed optimal resolutions to address harms committed by the Fuerzas Armadas Revolucionarias de Colombia—Ejército del Pueblo (FARC). Using thematic analysis, we identified three overall themes in adolescents' responses: (1) cynicism toward peace and desires for retribution, (2) improving the lives of those they thought were most impacted, and (3) restoring relationships and ending cycles of violence. We discuss the implications of our findings for psychological scholarship, peace education, and politics of reconciliation in light of Colombia's current political and social climate.
Inheritance and genetic mapping of the first CPMMV tolerance locus in common bean
Authors: Silva Rodrigo S. , Faria Josias C. , Vianello Rosana P. , Valdisser Paula Arielle M. R. , Pereira Helton S. , Melo Leonardo C. , Pinheiro Patrícia V. , Souza Thiago Lívio P. O. 
Published: March 2024

AbstractAlthough cowpea mild mottle virus (CPMMV) has been reported in Brazil since 1983, it has only become a significant concern for researchers and farmers in recent years. The objective of this work was to investigate the genetic basis of CPMMV tolerance, mapping and reporting the first loci associated with this trait in common bean (cv. BRS Sublime). Phenotypic assays were carried out on 180 individual plants (F2 generation) and 180 F2:3 progenies comprising 12 plants per family and their parents (BRS Sublime, tolerant parent × CNFCT 16207, susceptible parent). CPMMV was mechanically inoculated and symptoms were evaluated at 35 days after inoculation, using a 1–5 scoring scale. A linkage map was constructed using 1695 single‐nucleotide polymorphism (SNP) and SilicoDArT markers that segregated in the F2 and F2:3 generations as expected. Markers were distributed across 11 common bean chromosomes, resulting in a total length of 2864 cM, with an average distance between markers of 1.8 cM. Phenotypic observations revealed that tolerance in cv. BRS Sublime is controlled by a single dominant gene. The main effect quantitative trait locus (QTL; CPMMV.Pv08) associated with CPMMV tolerance was identified in the terminal region on chromosome 8 (Pv08). This QTL explained approximately 77% of phenotypic variation, indicating that the inheritance of tolerance to CPMMV is monogenic, controlled by a major locus. As far as we know, this study represents the first investigation into the inheritance and genetic mapping of CPMMV tolerance in common bean, with potential for the development of elite lines with multiple virus resistance/tolerance.
Use of a risk assessment tool to determine the origin of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)
Authors: Chen Xin , Kalyar Fatema , Chughtai Abrar Ahmad , MacIntyre Chandini Raina 
Published: March 2024

AbstractThe origin of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is contentious. Most studies have focused on a zoonotic origin, but definitive evidence such as an intermediary animal host is lacking. We used an established risk analysis tool for differentiating natural and unnatural epidemics, the modified Grunow–Finke assessment tool (mGFT) to study the origin of SARS‐COV‐2. The mGFT scores 11 criteria to provide a likelihood of natural or unnatural origin. Using published literature and publicly available sources of information, we applied the mGFT to the origin of SARS‐CoV‐2. The mGFT scored 41/60 points (68%), with high inter‐rater reliability (100%), indicating a greater likelihood of an unnatural than natural origin of SARS‐CoV‐2. This risk assessment cannot prove the origin of SARS‐CoV‐2 but shows that the possibility of a laboratory origin cannot be easily dismissed.
Intuitive toxicology in the 21st century—Bridging the perspectives of the public and risk assessors in Europe
Authors: Bearth Angela , Roth Nicolas , Wilks Martin F. , Siegrist Michael 
Published: March 2024

AbstractThree decades ago, several articles on the subjectivity in chemical risk judgments (i.e., labeled “intuitive toxicology”) measured the divide between the public and toxicologists with different backgrounds regarding the validity of predicting health effects based on in vivo studies. Similar divides with impacts on societal discourse and chemical risk assessment practices might exist concerning alternative toxicity testing methods (i.e., in vitro and in silico). However, studies to date have focused either on the public's views of in vivo or stem cell testing or on experts’ views of in vivo testing and potential alternatives (i.e., toxicologists and medical students), which do not allow for a direct investigation of potential divides. To fill this knowledge gap, we conducted two online surveys, involving members of the German‐speaking public in Switzerland and European human health risk assessors, respectively. This article presents the results of these two surveys regarding the divide in the public's and risk assessors’ perspectives on risk assessment based on in vivo, in vitro, and in silico testing. Particularly, the survey with the risk assessors highlights that, beyond scientific and regulatory barriers, alternatives to in vivo testing may encounter individual hurdles, such as higher uncertainty associated with them. Understanding and addressing these hurdles will be crucial to facilitate the integration of new approach methodologies into chemical risk assessment practices as well as a successful transition toward next‐generation risk assessment, bringing us closer to a fit‐for‐purpose and more efficient regulatory landscape.
Building stronger bonds: The impact of family support and communication on suicidal behaviors among Black men who have sex with men
Authors: Boyd Donte T. , Quinn Camille R. , Jones Kristian V. , Waller Bernadine , Coker Evelyn Joy , Duprey Erinn B. , Cerulli Catherine , McCoy Henrika 
Published: March 2024

AbstractIntroductionIt has been well documented that men who identify with a sexual orientation other than heterosexual are at a greater risk for suicide‐related outcomes. What is less known are the protective factors that can reduce such negative outcomes and contribute to their resilience.MethodsThis study used data collected between December 1, 2021, and January 2022 to understand how family factors contribute to or prevent depression symptoms and suicide outcomes among young Black men who have sex with men (BMSM) ages 18 to 29 (N = 400). A path analysis was conducted to explore the direct and indirect effects of suicide attempts.ResultsSurprisingly, there were nuanced findings that showed having a family member or friend die by suicide was indirectly associated with suicide planning and suicide attempts. It was also unexpectedly noted that there was a positive relationship between higher rates of depressive symptoms and higher levels of support from family members.ConclusionsThe population focused on in this study is understudied and has unique needs. Identifying familial support may not automatically reduce the thoughts and plans of young BMSM, which is an example of why their intersecting marginalized identities must be considered when conducting further research, creating interventions, and providing therapeutic services.
How to design and establish a national school‐based physical fitness monitoring and surveillance system for children and adolescents: A 10‐step approach recommended by the FitBack network
Authors: Joensuu Laura , Csányi Tamás , Huhtiniemi Mikko , Kälbi Katalin , Magalhães João , Milanović Ivana , Morrison Shawnda A. , Ortega Francisco B. , Sardinha Luis B. , Starc Gregor , Tammelin Tuija H. , Jurak Gregor ,   the FitBack Network
Published: March 2024

AbstractBackgroundProviding individual‐ and population‐level data on children's physical fitness (PF) is a crucial public health and education priority. However, few national fitness monitoring or surveillance systems are currently in practice internationally. We aim to summarize the current European PF monitoring and surveillance systems for school‐aged children and to provide experience‐based guidelines on how to design such systems.MethodsThe FitBack network consists of experts from diverse backgrounds with the common interest to improve the accessibility of PF monitoring for young people globally. Through FitBack network, we identified and compared the national or regional PF monitoring and surveillance systems currently in operation across Europe. We formulated a 10‐step approach for designing and establishing one's own system, based on analysis of experienced strengths, weaknesses, opportunities, and threats to monitoring childhood fitness.ResultsWe identified a total of eight PF monitoring systems in Finland, France, Galicia of Spain, Hungary, Lithuania, Portugal, Serbia, and Slovenia. The FitBack network recommends the following steps for designing and establishing one's own system: (1) set up mission statements and aims, (2) involve stakeholders, (3) utilize scientific background, (4) governance structure, (5) ensure sufficient funding, (6) data management planning, (7) provide meaningful feedback, (8) conduct pilot testing, (9) plan implementation process, and (10) invest in communication with stakeholders.ConclusionsThis study provides an updated overview of the best practices for school‐aged children's fitness monitoring and surveillance in Europe. Additionally, it offers a 10‐step approach to assist in the creation of similar systems in Europe or globally.
Effect of letermovir initiation on tacrolimus concentrations among lung transplant recipients receiving concomitant azole antifungal prophylaxis
Authors: Goodlet Kellie J. , Garcia Rhiannon 
Published: March 2024

AbstractBackgroundThe antiviral letermovir has been increasingly used as off‐label cytomegalovirus prophylaxis in solid organ transplant recipients. Observational studies have reported notable increases in tacrolimus (FK) exposure following letermovir; however, whether a significant interaction occurs in the setting of existing moderate‐to‐strong CYP3A4 inhibition is unknown. Therefore, the purpose of this study was to evaluate FK trough changes before and after letermovir among lung transplant recipients receiving azole antifungal prophylaxis.MethodsThis retrospective cohort study included lung transplant recipients newly initiated on letermovir between 2019–2022 following valganciclovir intolerance. Tacrolimus doses and concentrations were collected up to 30 days before and after the letermovir start date. No pre‐emptive FK dose adjustments occurred prior to letermovir initiation. Patients admitted to the hospital or lacking an appropriately timed trough in the pre‐ or post‐period were excluded.ResultsA total of 78 lung transplant recipients receiving FK (1.5 mg median total daily dose) and itraconazole (56.4%), isavuconazole (25.6%) or posaconazole (17.9%) prophylaxis were included. Letermovir was started at a median of 8.4 months post‐transplant. The pre‐/post‐letermovir median FK trough was 9.6/9.0 ng/mL (p = .151), median dose‐corrected trough was 4.2/4.7 ng/mL/mg (+11.9%, p = .032), and median weight‐based dose‐corrected trough was 362/326 [ng/mL]/[mg/kg/day] (‐9.9%, p = .036). There was no significant difference in the proportion of patients within their goal trough range before and after letermovir initiation (62% vs. 72%, p = .229).ConclusionEmpiric FK dose adjustments do not appear warranted before letermovir initiation in lung transplant recipients receiving antifungal prophylaxis with moderate‐to‐strong CYP3A4 inhibitors.
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Gibberellins regulate masculinization through the SpGAI‐SpSTM module in dioecious spinach
Authors: Zhang Yu‐Lan , Wang Li‐Ying , Yang Yi , Zhao Xu , Zhu Hong‐Wei , You Chen , Chen Ning , Wei Shuai‐Jie , Li Shu‐Fen , Gao Wu‐Jun 
Published: March 2024

SUMMARYThe sex of dioecious plants is mainly determined by genetic factors, but it can also be converted by environmental cues such as exogenous phytohormones. Gibberellic acids (GAs) are well‐known inducers of flowering and sexual development, yet the pathway of gibberellin‐induced sex conversion in dioecious spinach (Spinacia oleracea L.) remains elusive. Based on sex detection before and after GA3 application using T11A and SSR19 molecular markers, we confirmed and elevated the masculinization effect of GA on a single female plant through exogenous applications of GA3, showing complete conversion and functional stamens. Silencing of GIBBERELLIC ACID INSENSITIVE (SpGAI), a single DELLA family protein that is a central GA signaling repressor, results in similar masculinization. We also show that SpGAI can physically interact with the spinach KNOX transcription factor SHOOT MERISTEMLESS (SpSTM), which is a homolog of the flower meristem identity regulator STM in Arabidopsis. The silencing of SpSTM also masculinized female flowers in spinach. Furthermore, SpSTM could directly bind the intron of SpPI to repress SpPI expression in developing female flowers. Overall, our results suggest that GA induces a female masculinization process through the SpGAI‐SpSTM‐SpPI regulatory module in spinach. These insights may help to clarify the molecular mechanism underlying the sex conversion system in dioecious plants while also elucidating the physiological basis for the generation of unisexual flowers so as to establish dioecy in plants.
MaC2H2‐IDD regulates fruit softening and involved in softening disorder induced by cold stress in banana
Authors: Song Zunyang , Li Wenhui , Lai Xiuhua , Chen Hangcong , Wang Lihua , Chen Weixin , Li Xueping , Zhu Xiaoyang 
Published: March 2024

SUMMARYChilling stress causes banana fruit softening disorder and severely impairs fruit quality. Various factors, such as transcription factors, regulate fruit softening. Herein, we identified a novel regulator, MaC2H2‐IDD, whose expression is closely associated with fruit ripening and softening disorder. MaC2H2‐IDD is a transcriptional activator located in the nucleus. The transient and ectopic overexpression of MaC2H2‐IDD promoted “Fenjiao” banana and tomato fruit ripening. However, transient silencing of MaC2H2‐IDD repressed “Fenjiao” banana fruit ripening. MaC2H2‐IDD modulates fruit softening by activating the promoter activity of starch (MaBAM3, MaBAM6, MaBAM8, MaAMY3, and MaISA2) and cell wall (MaEXP‐A2, MaEXP‐A8, MaSUR14‐like, and MaGLU22‐like) degradation genes. DLR, Y1H, EMSA, and ChIP‐qPCR assays validated the expression regulation. MaC2H2‐IDD interacts with MaEBF1, enhancing the regulation of MaC2H2‐IDD to MaAMY3, MaEXP‐A2, and MaGLU22‐like. Overexpressing/silencing MaC2H2‐IDD in banana and tomato fruit altered the transcript levels of the cell wall and starch (CWS) degradation genes. Several differentially expressed genes (DEGs) were authenticated between the overexpression and control fruit. The DEGs mainly enriched biosynthesis of secondary metabolism, amino sugar and nucleotide sugar metabolism, fructose and mannose metabolism, starch and sucrose metabolism, and plant hormones signal transduction. Overexpressing MaC2H2‐IDD also upregulated protein levels of MaEBF1. MaEBF1 does not ubiquitinate or degrade MaC2H2‐IDD. These data indicate that MaC2H2‐IDD is a new regulator of CWS degradation in “Fenjiao” banana and cooperates with MaEBF1 to modulate fruit softening, which also involves the cold softening disorder.
Comparative and functional analysis unveils the contribution of photoperiod to DNA methylation, sRNA accumulation, and gene expression variations in short‐day and long‐day grasses
Authors: Wu Xia , Chen Siyi , Lin Feng , Muhammad Fahad , Xu Haiming , Wu Liang 
Published: March 2024

SUMMARYPhotoperiod employs complicated networks to regulate various developmental processes in plants, including flowering transition. However, the specific mechanisms by which photoperiod affects epigenetic modifications and gene expression variations in plants remain elusive. In this study, we conducted a comprehensive analysis of DNA methylation, small RNA (sRNA) accumulation, and gene expressions under different daylengths in facultative long‐day (LD) grass Brachypodium distachyon and short‐day (SD) grass rice. Our results showed that while overall DNA methylation levels were minimally affected by different photoperiods, CHH methylation levels were repressed under their favorable light conditions, particularly in rice. We identified numerous differentially methylated regions (DMRs) that were influenced by photoperiod in both plant species. Apart from differential sRNA clusters, we observed alterations in the expression of key components of the RNA‐directed DNA methylation pathway, DNA methyltransferases, and demethylases, which may contribute to the identified photoperiod‐influenced CHH DMRs. Furthermore, we identified many differentially expressed genes in response to different daylengths, some of which were associated with the DMRs. Notably, we discovered a photoperiod‐responsive gene MYB11 in the transcriptome of B. distachyon, and further demonstrated its role as a flowering inhibitor by repressing FT1 transcription. Together, our comparative and functional analysis sheds light on the effects of daylength on DNA methylation, sRNA accumulation, and gene expression variations in LD and SD plants, thereby facilitating better designing breeding programs aimed at developing high‐yield crops that can adapt to local growing seasons.
Intrinsic ankle stiffness is associated with paradoxical calf muscle movement but not postural sway or age
Authors: Reynolds Raymond F. , Liedtke Anna M. , Lakie Martin 
Published: March 2024

AbstractDue to Achilles tendon compliance, passive ankle stiffness is insufficient to stabilise the body when standing. This results in ‘paradoxical’ muscle movement, whereby calf muscles tend to shorten during forward body sway. Natural variation in stiffness may affect this movement. This may have consequences for postural control, with compliant ankles placing greater reliance upon active neural control rather than stretch reflexes. Previous research also suggests ageing reduces ankle stiffness, possibly contributing to reduced postural stability. Here we determine the relationship between ankle stiffness and calf muscle movement during standing, and whether this is associated with postural stability or age. Passive ankle stiffness was measured during quiet stance in 40 healthy volunteers ranging from 18 to 88 years of age. Medial gastrocnemius muscle length was also recorded using ultrasound. We found a significant inverse relationship between ankle stiffness and paradoxical muscle movement, that is, more compliant ankles were associated with greater muscle shortening during forward sway (r ≥ 0.33). This was seen during both quiet stance as well as voluntary sway. However, we found no significant effects of age upon stiffness, paradoxical motion or postural sway. Furthermore, neither paradoxical muscle motion nor ankle stiffness was associated with postural sway. These results show that natural variation in ankle stiffness alters the extent of paradoxical calf muscle movement during stance. However, the absence of a clear relationship to postural sway suggests that neural control mechanisms are more than capable of compensating for a lack of inherent joint stiffness.