bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of innovative epoxy resin and polyamine hardener microcapsules and their age monitoring by confocal Raman imaging
Authors: Aramayo Mariel Amparo Fernandez , Aoki Idalina Vieira 
Published: February 2024

AbstractWe present an innovative breakthrough encompassing synthesis, characterization, and age monitoring of epoxy resin and polyamine hardener microcapsules to form a dual‐component system for self‐healing anticorrosion coatings. The epoxy resin microcapsules (ER‐MC) are fabricated through oil–water emulsion and in situ polymerization, with poly (urea‐formaldehyde‐melamine) as shell material and a mixture of epoxy resin and n‐butyl glycidyl ether as core material. Modified aliphatic fast reactive polyamine hardener is microencapsulated with polymethylmethacrylate as shell using double emulsion and solvent evaporation method (PAH‐MC). Characterization includes optical microscopy, scanning electron microscopy, and particle size analyzer utilizing laser diffraction. The resulting microcapsules exhibited spherical shape with smooth surface devoid of porosity. Fourier transform infrared spectroscopy‐attenuated total reflection is utilized to confirm encapsulation by identifying the chemical structures of both microcapsule constituents. The thermogravimetric analysis further confirms the core content of microcapsules obtained from solvent extraction. ER‐MC contains approximately 66 wt %, while PAH‐MC stands 40 wt %, both showcasing remarkable thermal stability below 200°C. A ten‐month storage period fails to diminish the presence of the healing agents encapsulated within the polymeric walls, which is revealed by Raman spectroscopy utilizing 2D compositional mapping. This research demonstrates the viability of creating dual microcapsules and exemplify their potential for developing self‐healing coatings with dual‐component film‐forming healing agents.
Recent Advancements on Sustainable Electrochemical Water Splitting Hydrogen Energy Applications Based on Nanoscale Transition Metal Oxide (TMO) Substrates
Authors: Saeed Mohsin , Shahzad Umer , Marwani Hadi M. , Asiri Abdullah M. , ur Rehman Shujah , Althomali Raed H. , Rahman Mohammed M. 
Published: February 2024

AbstractThe development of green hydrogen generation technologies is increasingly crucial to meeting the growing energy demand for sustainable and environmentally acceptable resources. Many obstacles in the advancement of electrodes prevented water electrolysis, long thought to be an eco‐friendly method of producing hydrogen gas with no carbon emissions, from coming to fruition. Because of their great electrical conductivity, maximum supporting capacity, ease of modification in valence states, durability in hard environments, and high redox characteristics, transition metal oxides (TMOs) have recently captured a lot of interest as potential cathodes and anodes. Electrochemical water splitting is the subject of this investigation, namely the role of transition metal oxides as both active and supportive sites. It has suggested various approaches for the logical development of electrode materials based on TMOs. These include adjusting the electronic state, altering the surface structure to control its resistance to air and water, improving the flow of energy and matter, and ensuring the stability of the electrocatalyst in challenging conditions. In this comprehensive review, it has been covered the latest findings in electrocatalysis of the Oxygen Evolution Reaction (OER) and Hydrogen Evaluation Reaction (HER), as well as some of the specific difficulties, opportunities, and current research prospects in this field.
Rapid chemical reduction synthesis of copper nanoclusters with blue fluorescence for highly sensitive detection of furazolidone
Authors: Guo Yuyu , Wang Jiancheng , Zhang Lili , Wang Junkai 
Published: February 2024

AbstractTannic acid (TA), as a stabilizing agent, was successfully utilized to establish blue‐emitting copper nanoclusters (TA‐Cu NCs) on the basis of a facile chemical reduction preparation method. Characterization results proved successful synthesis of TA‐Cu NCs with uniform size and excellent stability. TA‐Cu NCs exhibited a blue emission wavelength at 431 nm when excited at 364 nm. Interestingly, the as‐prepared TA‐Cu NCs were selectively quenched by furazolidone based on static quenching. In addition, this analysis platform for furazolidone detection had an excellent linear range from 0.5 to 120 μM with a detection limit of 0.074 μM (S/N = 3). Furthermore, the accuracy of this sensing method was successfully confirmed by detecting furazolidone in bovine serum samples, indicating that TA‐Cu NCs had bright application prospects.
Engineering an Escherichia coli based in vivo mRNA manufacturing platform
Authors: Curry Edward , Muir George , Qu Jixin , Kis Zoltán , Hulley Martyn , Brown Adam 
Published: February 2024

AbstractSynthetic mRNA is currently produced in standardized in vitro transcription systems. However, this one‐size‐fits‐all approach has associated drawbacks in supply chain shortages, high reagent costs, complex product‐related impurity profiles, and limited design options for molecule‐specific optimization of product yield and quality. Herein, we describe for the first time development of an in vivo mRNA manufacturing platform, utilizing an Escherichia coli cell chassis. Coordinated mRNA, DNA, cell and media engineering, primarily focussed on disrupting interactions between synthetic mRNA molecules and host cell RNA degradation machinery, increased product yields >40‐fold compared to standard “unengineered” E. coli expression systems. Mechanistic dissection of cell factory performance showed that product mRNA accumulation levels approached theoretical limits, accounting for ~30% of intracellular total RNA mass, and that this was achieved via host‐cell's reallocating biosynthetic capacity away from endogenous RNA and cell biomass generation activities. We demonstrate that varying sized functional mRNA molecules can be produced in this system and subsequently purified. Accordingly, this study introduces a new mRNA production technology, expanding the solution space available for mRNA manufacturing.
Clinical practice guidelines for full‐cycle standardized management of bone health in breast cancer patients
Authors:   Committee of Full‐cycle Standardized Management of Bone Health in Breast Cancer Patients
Published: February 2024

AbstractBone health management for breast cancer spans the entire cycle of patient care, including the prevention and treatment of bone loss caused by early breast cancer treatment, the adjuvant application of bone‐modifying agents to improve prognosis, and the diagnosis and treatment of advanced bone metastases. Making good bone health management means formulating appropriate treatment strategies and dealing with adverse drug reactions, and will help to improve patients' quality of life and survival rates. The Breast Cancer Expert Committee of the National Cancer Center for Quality Control organized relevant experts to conduct an in‐depth discussion on the full‐cycle management of breast cancer bone health based on evidence‐based medicine, and put forward reasonable suggestions to guide clinicians to better deal with health issues in bone health clinics.
Exploring women's experiences of healing from sexual trauma through engagement in mind–body practices: A systematic review
Authors: Nixon Molly Ann 
Published: February 2024

AbstractObjectiveThis systematic review is the first to consolidate qualitative findings exploring women's healing experiences from sexual trauma through engagement in mind–body practices. This review aimed to expand our knowledge regarding the healing processes for sexual trauma and to help move towards further integration of holistic practices in trauma therapies.MethodA methodical literature search was conducted using four databases: PsycINFO, Medline, CINAHL Plus and Scopus. After the initial search, 2392 records were assessed for eligibility. After screening, 11 articles remained and were analysed using thematic synthesis.FindingsData synthesis led to the conceptualisation of five main themes and three subthemes: (1) Integrating Mind and Body—(a) Reconnecting to the Body and (b) Processing Emotions; (2) Self‐compassion; (3) Trauma‐Sensitive Approach; (4) Connection—(a) Relationships Within the Group, (b) Safety in Relationships and (c) Spiritual Connection; and (5) Fun and Spontaneity.ConclusionsThe findings highlight the potential healing processes of body‐based practices for women subjected to sexual violence. The review provides evidence for body‐based practices focussing on holistic growth to be used in adjunction to therapy for sexual trauma. Limitations and future research are discussed.
New 1H‐1,2,4‐Triazolyl Derivatives as Antimicrobial Agents
Authors: Sucman Natalia , Stingaci Eugenia , Lupascu Lucian , Smetanscaia Anastasia , Valica Vladimir , Uncu Livia , Shova Sergiu , Petrou Anthi , Glamočlija Jasmina , Soković Marina , Geronikaki Athina , Macaev FliurZ 
Published: February 2024

AbstractNew 1H‐1,2,4‐triazolyl derivatives were synthesized, and six of them were selected based on docking prediction for the investigation of their antimicrobial activity against five bacterial and eight fungal strains. All compounds demonstrated antibacterial activity with MIC lower than that of the ampicillin and chloramphenicol. In general, the most sensitive bacteria appeared to be P. fluorescens, while the plant pathogen X. campestris was the most resistant. The antifungal activity of the compounds was much better than the antibacterial activity. All compounds were more potent (6 to 45 times) than reference drugs ketoconazole and bifonazole with the best activity achieved by compound 4 a. A. versicolor, A. ochraceus, A.niger, and T.viride showed the highest sensitivity to compound 4 b, while, T. viride, P. funiculosum, and P.ochrochloron showed good sensitivity to compound 4 a. Molecular docking studies suggest that the probable mechanism of antibacterial activity involves the inhibition of the MurB enzyme of E. coli, while CYP51 of C. albicans appears to be involved in the mechanism of antifungal activity. It is worth mentioning that none of the tested compounds violated Lipinski's rule of five.
Lachnuoic Acids A−F: Ambuic Acid Congeners from a Saprotrophic Lachnum Species
Authors: Phutthacharoen Kunthida , Toshe Rita , Khalid Syeda J. , Llanos‐López Natalia A. , Wennrich Jan‐Peer , Schrey Hedda , Ebada Sherif S. , Hyde Kevin D. , Stadler Marc 
Published: February 2024

AbstractChemical prospection of an extract derived from a saprotrophic fungus Lachnum sp. IW157 resulted in the isolation and characterization of six unprecedentedly reported ambuic acid analogues named lachnuoic acids A−F (1–6). Chemical structures of 1–6 were determined based on comprehensive 1D and 2D NMR spectroscopic analyses together with HR‐ESI‐MS spectrometry. The relative configurations of 1–3 were defined by ROESY spectroscopic analyses while their absolute configurations were unambiguously determined by Mosher's esters method. All isolated compounds were subjected to cytotoxic, antimicrobial, antibiofilm and nematicidal activity assays where only lachnuoic acid A (1) revealed potent antimicrobial activity against Staphylococcus aureus and Bacillus subtilis at MIC values of 16.6 and 8.3 μg/mL, respectively.
Epigenetic changes in shear‐stressed endothelial cells
Authors: Pinto Thaís Silva , Feltran Geórgia da Silva. , Fernandes Célio Júnior da C. , de Camargo Andrade Amanda Fantini , Coque Alex de Camargo , Silva Simone L. , Abuderman Abdulwahab A. , Zambuzzi Willian F. , Foganholi da Silva Rodrigo A. 
Published: February 2024

AbstractEpigenetic changes, particularly histone compaction modifications, have emerged as critical regulators in the epigenetic pathway driving endothelial cell phenotype under constant exposure to laminar forces induced by blood flow. However, the underlying epigenetic mechanisms governing endothelial cell behavior in this context remain poorly understood. To address this knowledge gap, we conducted in vitro experiments using human umbilical vein endothelial cells subjected to various tensional forces simulating pathophysiological blood flow shear stress conditions, ranging from normotensive to hypertensive forces. Our study uncovers a noteworthy observation wherein endothelial cells exposed to high shear stress demonstrate a decrease in the epigenetic marks H3K4ac and H3K27ac, accompanied by significant alterations in the levels of HDAC (histone deacetylase) proteins. Moreover, we demonstrate a negative regulatory effect of increased shear stress on HOXA13 gene expression and a concomitant increase in the expression of the long noncoding RNA, HOTTIP, suggesting a direct association with the suppression of HOXA13. Collectively, these findings represent the first evidence of the role of histone‐related epigenetic modifications in modulating chromatin compaction during mechanosignaling of endothelial cells in response to elevated shear stress forces. Additionally, our results highlight the importance of understanding the physiological role of HOXA13 in vascular biology and hypertensive patients, emphasizing the potential for developing small molecules to modulate its activity. These findings warrant further preclinical investigations and open new avenues for therapeutic interventions targeting epigenetic mechanisms in hypertensive conditions.
The ultrastructure of the apical organ of Curini‐Galletti's larva, a new polyclad larval type
Authors: Dittmann Isabel L. , Bertemes Philip , Gotsis Clemens , Grosbusch Alexandra L. , Redl Stefan , Hess Michael W. , Salvenmoser Willi , Egger Bernhard 
Published: February 2024

AbstractPolycladida are the only free‐living flatworms with a planktonic larval stage in some species. Currently, it is not clear if a larval stage is ancestral in polyclads, and which type of larva that would be. Known polyclad larvae are Müller's larva, Kato's larva and Goette's larva, differing by body shape and the number of lobes and eyes. A valuable character for the comparison and characterisation of polyclad larval types is the ultrastructural composition of the apical organ. This organ is situated at the anterior pole of the larva and is associated with at least one ciliary tuft. The larval apical organ of Theama mediterranea features two multiciliated apical tuft sensory cells. Six unfurcated apical tuft gland cell necks are sandwiched between the apical tuft sensory cells and two anchor cells and have their cell bodies located lateral to the brain. Another type of apical gland cell necks is embedded in the anchor cells. Ventral to the apical tuft, ciliated sensory neurons are present, which are neighbouring the cell necks of two furcated apical tuft gland cells. Based on the ultrastructural organisation of the apical organ and other morphological features, like a laterally flattened wedge‐shaped body and three very small lobes, we recognise the larva of T. mediterranea as a new larval type, which we name Curini‐Galletti's larva after its first discoverer. The ultrastructural similarities of the apical organ in different polyclad larvae support their possible homology, that is, all polyclad larvae have likely evolved from a common larva.
Assessment of human embryonic stem cells differentiation into definitive endoderm lineage on the soft substrates
Authors: Virdi Jasmeet Kaur , Pethe Prasad 
Published: February 2024

AbstractPluripotent stem cells (PSCs) hold enormous potential for treating multiple diseases owing to their ability to self‐renew and differentiate into any cell type. Albeit possessing such promising potential, controlling their differentiation into a desired cell type continues to be a challenge. Recent studies suggest that PSCs respond to different substrate stiffness and, therefore, can differentiate towards some lineages via Hippo pathway. Human PSCs can also differentiate and self‐organize into functional cells, such as organoids. Traditionally, human PSCs are differentiated on stiff plastic or glass plates towards definitive endoderm and then into functional pancreatic progenitor cells in the presence of soluble growth factors. Thus, whether stiffness plays any role in differentiation towards definitive endoderm from human pluripotent stem cells (hPSCs) remains unclear. Our study found that the directed differentiation of human embryonic stem cells towards endodermal lineage on the varying stiffness did not differ from the differentiation on stiff plastic dishes. We also observed no statistical difference between the expression of yes‐associated protein (YAP) and phosphorylated YAP. Furthermore, we demonstrate that lysophosphatidic acid, a YAP activator, enhanced definitive endoderm formation, whereas verteporfin, a YAP inhibitor, did not have the significant effect on the differentiation. In summary, our results suggest that human embryonic stem cells may not differentiate in response to changes in stiffness, and that such cues may not have as significant impact on the level of YAP. Our findings indicate that more research is needed to understand the direct relationship between biophysical forces and hPSCs differentiation.
2024 Statement from Asia expert operators on transcatheter pulmonary valve replacement
Authors: Pan Wenzhi , Zhou Daxin , Hijazi Ziyad M. , Qureshi Shakeel A. , Promphan Worakan , Feng Yuan , Zhang Gejun , Liu Xianbao , Pan Xin , Chen Lianglong , Cao Qilin , Tiong Koh G. , Leong Ming C. , Roymanee Supaporn , Prachasilchai Pimpak , Choi Jae Y. , Tomita Hideshi , Le Tan Ju , Akhtar Khurram , Lam Simon , So Kent , Tin Do N. , Nguyen Lan Hieu , Huo Yong , Wang Jian'an , Ge Junbo 
Published: February 2024

AbstractTranscatheter pulmonary valve replacement (TPVR), also known as percutaneous pulmonary valve implantation, refers to a minimally invasive technique that replaces the pulmonary valve by delivering an artificial pulmonary prosthesis through a catheter into the diseased pulmonary valve under the guidance of X‐ray and/or echocardiogram while the heart is still beating not arrested. In recent years, TPVR has achieved remarkable progress in device development, evidence‐based medicine proof and clinical experience. To update the knowledge of TPVR in a timely fashion, and according to the latest research and further facilitate the standardized and healthy development of TPVR in Asia, we have updated this consensus statement. After systematical review of the relevant literature with an in‐depth analysis of eight main issues, we finally established eight core viewpoints, including indication recommendation, device selection, perioperative evaluation, procedure precautions, and prevention and treatment of complications.
Electrochemical Oxidation of Aliphatic Carboxylates: Kinetics, Thermodynamics, Mechanism, and the Role of Hydrogen Bonding
Authors: Abdel Latif Marwa K. , Spencer Jared N. , Kidd Bryce E. , Madsen Louis A. , Tanko James M. 
Published: February 2024

AbstractThe oxidation of tetra‐n‐butylammonium acetate, propionate, and pivalate was studied in rigorously anhydrous acetonitrile by conventional linear sweep and convolution voltammetry (LSV and ConV, respectively). The results suggest oxidation occurs via a concerted dissociative electron transfer pathway (RCO2−→R⋅+CO2+e−). The addition of water lowers the intrinsic barrier, signaling a possible change in mechanism to stepwise dissociative electron transfer. In rigorously dry acetonitrile, the oxidation potentials of CH3CO2− (0.60±0.09), CH3CH2CO2−, (0.47±0.05) and (CH3)3CCO2− (0.40±0.04 V vs. Ag/AgNO3 (CH3CN, 0.1 M)) are reported. These values parallel the stabilities of the resulting free radicals, consistent with a possible concerted pathway, although differential solvation of the carboxylate anions cannot be completely excluded as a contributor to this trend.
A tale of two pathways: Review of immune checkpoint inhibitors in DNA mismatch repair‐deficient and microsatellite instability‐high endometrial cancers
Authors: Liu Ying L. , Weigelt Britta 
Published: February 2024

AbstractThe DNA mismatch repair (MMR) pathway is critical for correcting DNA mismatches generated during DNA replication. MMR‐deficiency (MMR‐D) leads to microsatellite instability (MSI) associated with an increased mutation rate, driving cancer development. This is particularly relevant in endometrial cancer (EC) as 25%–30% of tumors are of MMR‐D/MSI‐high (MSI‐H) phenotype. Comprehensive assessment using immunohistochemistry (IHC) and sequencing‐based techniques are necessary to fully evaluate ECs given the importance of molecular subtyping in staging and prognosis. This also influences treatment selection as clinical trials have demonstrated survival benefits for immune checkpoint inhibitors (ICIs) alone and in combination with chemotherapy for MMR‐D/MSI‐H EC patients in various treatment settings. As a portion of MMR‐D/MSI‐H ECs are driven by Lynch syndrome, an inherited cancer predisposition syndrome that is also associated with colorectal cancer, this molecular subtype also prompts germline assessment that can affect at‐risk family members. Additionally, heterogeneity in the tumor immune microenvironment and tumor mutation burden (TMB) have been described by MMR mechanism, meaning MLH1 promoter hypermethylation versus germline/somatic MMR gene mutation, and this may affect response to ICI therapies. Variations by ancestry in prevalence and mechanism of MMR‐D/MSI‐H tumors have also been reported and may influence health disparities given observed differences in tumors of Black compared to White patients which may affect ICI eligibility. These observations highlight the need for additional prospective studies to evaluate the nuances regarding MMR‐D heterogeneity as well as markers of resistance to inform future trials of combination therapies to further improve outcomes for patients with EC.
Joint optimization of load balancing and resource allocation in cloud environment using optimal container management strategy
Authors: Muniswamy Saravanan , Vignesh Radhakrishnan 
Published: February 2024

SummaryDue to the high performance of cloud computing‐based microservices, a wide range of industries and fields rely on them. In a containerized cloud, traditional resource management strategies are typically used to allocate and migrate virtual machines. A major problem for cloud service providers is resource allocation for containers, which directly affects system performance and resource consumption. In this paper, we propose a joint optimization of load balancing and resource allocation in the cloud using an optimal container management strategy. We aim to enhance scheduling efficiency and reduce costs by improving the container's schedule requested digitally by users. An improved backtracking search optimization (IBSO) algorithm is used to allocate resources between end‐users/IoT devices and the cloud under the consideration of service‐level agreements. Mechanic quantum recurrent neural networks (MQ‐RNNs) are designed to allocate, consolidate, and migrate containers in cloud environments. The various simulation measures used to validate the proposed strategy are energy consumption, number of active servers, number of interruptions, total cost, runtime, and statistical measures.