bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of TGFBI-TAGLN axis in cancer stem cell property of head and neck squamous cell carcinoma
Authors: Sarubo Motoharu , Mouri Yasuhiro , Moromizato Akira , Yamada Azusa , Jin Shengjan , Shao Wenhua , Hagita Hiroko , Miyoshi Keiko , Kudo Yasusei 
Published: March 2024

AbstractHead and neck squamous cell carcinoma (HNSCC) is a significant healthcare burden globally. Previous research using single-cell transcriptome analysis identified TGFBI as a crucial marker for the partial-epithelial-mesenchymal transition (partial-EMT) program. However, the precise role of TGFBI in HNSCC progression remains unclear. Therefore, our study aimed to clarify the impact of TGFBI on the malignant behavior of HNSCC cells. Through RNA-sequencing data from the TCGA database, we validated that increased TGFBI expression correlates with a higher occurrence of lymph node metastasis and unfavorable prognosis in HNSCC cases. Functional experiments demonstrated that TGFBI overexpression enhances the ability of sphere formation, indicating stem-cell-like properties. Conversely, TGFBI depletion reduces sphere formation and suppresses the expression of cancer stem cell (CSC) markers. RNA-sequencing analysis of TGFBI-overexpressing and control HNSCC cells revealed TAGLN as a downstream effector mediating TGFBI-induced sphere formation. Remarkably, TAGLN depletion abolished TGFBI-induced sphere formation, while its overexpression rescued the suppressed sphere formation caused by TGFBI depletion. Moreover, elevated TAGLN expression showed correlations with the expression of TGFBI and partial-EMT-related genes in HNSCC cases. In conclusion, our findings suggest that TGFBI may promote CSC properties through the upregulation of TAGLN. These novel insights shed light on the involvement of the TGFBI-TAGLN axis in HNSCC progression and hold implications for the development of targeted therapies.
Short-term restoration practices change the bacterial community in degraded soil from the Brazilian semiarid
Authors: Silva Davila Esmelinda Oliveira , Costa Romario Martins , Campos Janaira Rocha , Rocha Sandra Mara Barbosa , de Araujo Pereira Arthur Prudencio , Melo Vania Maria Maciel , Oliveira Francisca Andrea Silva , de Alcantara Neto Francisco , Mendes Lucas William , Araujo Ademir Sergio Ferreira 
Published: March 2024

AbstractLand degradation by deforestation adversely impacts soil properties, and long-term restoration practices have been reported to potentially reverse these effects, particularly on soil microorganisms. However, there is limited knowledge regarding the short-term effects of restoration on the soil bacterial community in semiarid areas. This study evaluates the bacterial community in soils experiencing degradation (due to slash-and-burn deforestation) and restoration (utilizing stone cordons and revegetation), in comparison to a native soil in the Brazilian semiarid region. Three areas were selected: (a) under degradation; (b) undergoing short-term restoration; and (c) a native area, and the bacterial community was assessed using 16S rRNA sequencing on soil samples collected during both dry and rainy seasons. The dry and rainy seasons exhibited distinct bacterial patterns, and native sites differed from degraded and restoration sites. Chloroflexi and Proteobacteria phyla exhibited higher prevalence in degraded and restoration sites, respectively, while Acidobacteria and Actinobacteria were more abundant in sites undergoing restoration compared to degraded sites. Microbial connections varied across sites and seasons, with an increase in nodes observed in the native site during the dry season, more edges and positive connections in the restoration site, and a higher occurrence of negative connections in the degradation site during the rainy season. Niche occupancy analysis revealed that degradation favored specialists over generalists, whereas restoration exhibited a higher prevalence of generalists compared to native sites. Specifically, degraded sites showed a higher abundance of specialists in contrast to restoration sites. This study reveals that land degradation impacts the soil bacterial community, leading to differences between native and degraded sites. Restoring the soil over a short period alters the status of the bacterial community in degraded soil, fostering an increase in generalist microbes that contribute to enhanced soil stability.
A novel papillomavirus in a New Zealand fur seal (Arctocephalus forsteri) with oral lesions
Authors: Mifsud Jonathon C. O. , Hall Jane , Van Brussel Kate , Rose Karrie , Parry Rhys H. , Holmes Edward C. , Harvey Erin 
Published: March 2024

AbstractDespite being the predominant seal species in the Australian-New Zealand region and serving as a key indicator of marine environmental health, little is known about infectious diseases in New Zealand fur seals (Long-nosed fur seal; Arctocephalus forsteri). Several papillomaviruses have been identified in earless seals and sea lions, with the latter linked to cutaneous plaques and invasive squamous cell carcinoma. To date, no papillomaviruses have been reported in fur seals. We used traditional veterinary diagnostic techniques and metatranscriptomic sequencing of tissue samples to investigate the virome of New Zealand fur seals. We identified a novel papillomavirus, provisionally termed A. forsteri papillomavirus 1 (AforPV1) in an animal with clinically and histologically identified oral papilloma-like lesions. RT-PCR confirmed the presence of AforPV1 only in oral papilloma samples from the affected individual. Phylogenetic analysis of the complete 7926 bp genome of AforPV1 revealed that it grouped with taupapillomaviruses found in related Carnivora species. These findings highlight the need for further research into the disease associations and impact of undiagnosed and novel viruses on New Zealand fur seals.
Multiwavelength study of the HII region LHA 120-N11 in the Large Magellanic Cloud with eROSITA
Authors: Tsuge K. , Sasaki M. , Knies J. R. , Haberl F. , Points S. , Maitra C. , Filipović M. D. , Staveley-Smith L. , Koribalski B. S. , Kerp J. 
Published: March 2024

Aims. We studied the diffuse X-ray emission around the HII region LHA 120-N11, which is one of the most active star-forming regions in the Large Magellanic Cloud. We want to determine the nature of the diffuse X-ray emission and improve our understanding of its origin including related interactions with the cold interstellar medium.
Methods. We analyzed the diffuse X-ray emission observed with the extended Roentgen Survey with an Imaging Telescope Array (eROSITA) on the Spectrum-Roentgen-Gamma mission to determine the physical properties of the hot diffuse X-ray emission. Four spectral extraction regions were defined based on the morphology of the X-ray emission. We also studied HI and CO data, as well as Hα line emission in the optical, and compared them with the properties of the diffuse X-ray emission.
Results. The X-ray emission in the four regions is well fitted with an absorbed model consisting of thermal plasma models (vapec) yielding temperatures of kT = ~0.2 keV and kT = 0.8–1.0 keV. The comparison of the X-ray absorption column density and the hydrogen column density shows that the X-ray dark lane located north of N11 is apparently caused by the absorption by HI and CO clouds. By estimating the energy budget of the thermal plasma, we also investigated the heating mechanism of the X-ray emitting plasma. The energy of the diffuse X-ray emission in the superbubble which is a star-forming bubble with a radius of ~120 pc including OB associations LH9, LH10, LH11, and LH13 can be explained by heating from high-mass stars. In the surrounding regions we find that the energy implied by the X-ray emission suggests that additional heating might have been caused by shocks generated by cloud–cloud collisions.
Tidal frequency dependence of the Saturnian k2 Love number
Authors: Lainey V. , Dewberry J. W. , Fuller J. , Cooper N. , Rambaux N. , Zhang Q. 
Published: March 2024

Context. Love numbers describe the fluid and elastic response of a body to the tidal force of another massive object. By quantifying these numbers, we can more accurately model the interiors of the celestial objects concerned.
Aims. We determine Saturn’s degree-2 Love number, k2, at four different tidal forcing frequencies.
Methods. To do this, we used astrometric data from the Cassini spacecraft and a dynamical model of the orbits of Saturn’s moons.
Results. The values obtained for k2 are 0.384 ± 0.015, 0.370 ± 0.023, 0.388 ± 0.006, and 0.376 ± 0.007 (1σ error bar) for the tidal frequencies of Janus–Epimetheus, Mimas, Tethys, and Dione.
Conclusions. We show that these values are compatible with a constant Love number formulation. In addition, we compared the observed values with models of dynamical tides excited in Saturn’s interior, also finding a good agreement. Future increases in the measurement precision of Love numbers will provide new constraints on the internal structure of Saturn.
Epos EPO
Authors: Naas Stephanie , Schödel Johannes , Grampp Steffen 
Published: March 2024

ZUSAMMENFASSUNGDie Substitution des Hormons Erythropoetin (EPO) stellte bisher den Grundpfeiler der Therapie der renalen Anämie bei nierenkranken Patienten*innen dar. Die seit über 30 Jahren in der Praxis etablierte Anwendung macht die verwendeten rekombinanten Substanzen zu Medikamenten, für die ein reicher Erfahrungsschatz vorliegt. Aufgrund seiner umschriebenen Wirkweise, des bekannten Nebenwirkungsprofils sowie der aktuellen Studienlage, die bisher überwiegend eine Gleichwertigkeit gegenüber neuen Erythropoese stimulierenden Agenzien (ESA) zeigt, ist wohl kein Ende der EPO-Substitutionstherapie abzusehen. Die Therapie der renalen Anämie wird allerdings durch neue Erkenntnisse zum Eisenhaushalt sowie die nun zugelassenen HIF-Stabilisatoren optimiert und individualisiert werden können.
Einfluss des Refluxursprungs auf die Hämodynamik, Klinik und Therapie der primären Varikose
Authors: Wilmanns Christoph , Zechner Ulrich , Walter Paul Karl , Schulze Alicia 
Published: March 2024

ZusammenfassungDie Mechanismen von Reflux und Rezirkulation der primären Varikose sind nach wie vor nicht vollständig geklärt.Als Refluxursachen wurden ein junktionaler Phänotyp (JP, Mündungsreflux der V. saphena magna [VSM oder Leistenrezidiv] mit oder ohne suspekte Perforansvenen [SPV]) und ein Perforansphänotyp (PP, ausschließlich SPV oder aus Gründen der statistischen Zuordnung die V. saphena parva [VSP] als Refluxquelle) gegeneinander abgegrenzt. Flussrichtung und -stärke wurden unter Valsalva (JP) oder als spontan/unter distaler Kompression/Dekompression (SPV) erfasst und im Falle von SPV mit jeweils 1 oder 2 Punkten als Reflux (+) oder Reentry (−) mit resultierenden möglichen Nettoflüssen von +6 bis −6 Punkten bewertet. Refluxquelle und axiale Refluxausdehnung sowie Durchmesser und Flussrichtung von SPV wurden multivariat mit den klinischen Stadien nach CEAP-Klassifikation verglichen.An 107 unteren Extremitäten fand sich 68× JP, in 49 Fällen davon vergesellschaftet mit SPV, und 39× PP. CEAP C3–C6 war im Falle von 45/65 (11/22) Erstmanifestationen (Rezidiven) mit SPV (JP und PP) vergesellschaftet oder in 3/16 (0/4) ohne, p < 0,01 (p = 0,01). Fortgeschrittene klinische Stadien C4–C6 waren im Falle einer Erstmanifestation von JP häufiger vertreten, wenn der axiale Reflux bis distal (14/39; p = 0,01) oder proximal des Kniegelenkes (3/11; p = 0,12) reichte verglichen mit PP (5/31). Die Flussrichtung von SPV war im Falle einer Erstmanifestation von JP sowie axialem Reflux bis distal des Kniegelenkes Reentry (Schätzer −1,62; p = 0,02) oder bis proximal des Kniegelenkes Reflux (0,29; p = 0,81) verglichen mit PP. Der Durchmesser der am meisten dilatierten Perforansvene war jedoch größer im Falle von JP und axialem Reflux bis proximal (Schätzer 0,20; p < 0,01) oder distal des Kniegelenkes (0,04; p = 0,30) verglichen mit PP. Vorherrschende Flussrichtungen
von SPV waren Reentry bzw. Reflux während peripherer Kompression oder Dekompression (p = 0,009).Die Daten sprechen für einen Zusammenhang zwischen Refluxquelle und Ausdehnung eines axialen Refluxes mit dem Durchmesser und der Flussrichtung von SPV sowie dem klinischen Stadium der primären Varikose.
Preclinical Targeting of the PGRMC1-CK2 Axis with Silmitasertib: A
Potential Strategy for Lung Adenocarcinoma Therapy
Authors: Solaipriya S. , Anbalagan M , Sivaramakrishnan V. 
Published: March 2024

AbstractProgesterone receptor membrane component 1 (PGRMC1) is a pleiotropic protein
over-expressed in lung adenocarcinoma (LUAD). The precise molecular mechanisms
underlying the signature motif of Casein kinase (CK2) presence in PGRMC1 and
their role in LUAD remain unclear. X-ray crystallographic structure for CK2 and
PGRMC1 from the PubChem database was obtained and subjected to protein-protein
interaction (PPI) analysis to identify their interactions. In addition, the CK2
inhibitor – Silmitasertib was also utilised to understand the interaction
between PGRMC1-CK2. The PPI complex (PGRMC1-CK2) and the PPI-ligand interaction
analysis and their Molecular Dynamics (MD) studies revealed the stability of
their interactions and critical amino acid contacts within the 5Ǻ vicinity of
the CK2 signature motif "T/S-x-x-E/D". Moreover, in-vitro
colony formation assay, migration assay, and gene expression analysis using
quantitative Real-time PCR revealed that Silmitasertib (IC50–2.5 μM)
was highly influential in suppressing the PGRMC1-CK2 expression axis. In
conclusion, our study infers that PGRMC1-CK-2 axis inhibition could be a
potential therapeutic option to limit the promotion and progression of lung
cancer.
Kollaps auf Raten?
Authors: Koch Peter 
Published: March 2024

ZusammenfassungIn der stationären Langzeitpflege wurden in den vergangenen Jahren die Rationalisierungsreserven, sprich
das Personal, ziemlich ausgeschöpft. Jetzt steht auch hier das System vor einem Kollaps. Lesen Sie mehr
über die Chronologie der Langzeitpflege.
Pediatric Consent on FHIR
Authors: Voronov Anton , Jafari Mohammad , Zhao Lin , Soliz Melissa , Hong Qixuan , Pope John , Chern Darwyn , Lipman Megan , Grando Adela 
Published: March 2024

Abstract
Background Standardizing and formalizing consent processes and forms can prevent ambiguities, convey a more precise meaning, and support machine interpretation of consent terms.
Objectives Our goal was to introduce a systematic approach to standardizing and digitizing pediatric consent forms, which are complex due to legal requirements for child and legal guardian involvement.
Methods First, we reviewed the consent requirements from the Arizona regulation, and we used 21 pediatric treatment consents from five Arizona health care organizations to propose and evaluate an implementation-agnostic Consent for Treatment Framework. Second, we assessed the adequacy of the Fast Healthcare Interoperability Resources (FHIR) to support the proposed framework.
Results The resulting Consent for Treatment Framework supports compliance with the state consent requirements and has been validated with pediatric consent forms. We also demonstrated that the FHIR standard has the required expressiveness to compute the framework's specifications and express the 21 consent forms.
Conclusion Health care organizations can apply the shared open-source code and FHIR implementation guidelines to standardize the design of machine-interpretable pediatric treatment consent forms. The resulting FHIR-based executable models may support compliance with the law and support interoperability and data sharing.
Giant Cutaneous Leiomyoma of Scalp
Authors: Raj Sumit , Joshi Deepti , Agrawal Amit , Mishra Rakesh , Shrivastav Adesh , Chouksey Pradeep , Saha Kaustav 
Published: March 2024

AbstractLeiomyomas are benign tumors arising from smooth muscle, most commonly seen in uterine myometrium, gastrointestinal tract, skin, and lower extremities of middle-aged women. Leiomyomas of head and neck region account for less than 1% of all leiomyomas. The most common site of leiomyoma in the head and neck region is the lips followed by tongue, and other maxillofacial regions. The clinical features, etiology, differential diagnosis, and treatment of leiomyoma are discussed in this case report. The aim of this case report is to raise awareness about a rare form of scalp giant leiomyoma. This could expand its consideration as a possible cause of uncertain neoplasms and promote accurate clinical diagnosis, leading to better treatment results.
SFM Fetal Therapy Practice Guidelines: Fetal Fluid Aspiration
Authors: Gupta Sangeeta , Batra Meenu 
Published: March 2024

AbstractFetal fluid aspiration is commonly required from the pleural cavity or bladder and is termed as thoracocentesis or vesicocentesis, respectively. These procedures are performed for diagnostic and therapeutic indications. A detailed pre-procedure counseling and informed consent of the patient are necessary explaining the procedure and risks involved. These can be performed under aseptic precautions using ultrasound guidance with the help of 22-gauge needle.
Ruptured Mycotic Thoracic Aortic Aneurysm in the Setting of Streptococcus Bacteremia with Underlying Colonic Malignancy
Authors: Chait Jesse , Mendes Bernardo C. 
Published: March 2024

AbstractRuptured mycotic thoracic aortic aneurysms (TAAs) pose complex clinical challenges which are often compounded by existing comorbidities of the typical patient. We present the case of an 85-year-old female presenting emergently with a ruptured mycotic TAA with underlying Streptococcus bacteremia who was successfully treated with a thoracic endograft and antibiotics.
Development and Validation of Information Technology Scale in Nursing
Authors: Sarac Elif , Yildiz Esra 
Published: March 2024

Abstract
Background The implementation of information technology (IT) in patient care is on the rise. The nursing workforce should be prepared for using such technology to support the delivery of patient-centered care. The integration of informatics into nursing practice has been progressing at a slower rate than the development of advancements and in which areas nurses use IT is still not clear.
Objective Our objective was to develop a new instrument to determine the usage of IT in nursing practice.
Methods A methodological study was conducted with factor analyses. A total of 498 registered nurses in a university hospital (n = 374) and primary care centers (n = 124) participated in the study. A questionnaire consisting demographic characteristics and an item pool with 50 statements were used to collect data. The validity and reliability of the instrument were statistically tested by computing the Keiser–Meier–Olkin (KMO) and Bartlett tests, an exploratory factor analysis, descriptive statistics, Cronbach's α, and a confirmatory factor analysis.
Results The instrument extracted eight factors comprising 39 items that explained 55% of the variance: professional autonomy(α = 0.82), data sharing/communication(α = 0.80), data management (α = 0.79), professional development (α = 0.71), administration (α = 0.76), research (α = 0.76), informing (α = 0.68), and classification of interventions (α = 0.75). Total reliability was 0.936. KMO index and a measure of sampling adequacy were high (0.936); the Bartlett test of sphericity was significant (p < 0.005).
Conclusion Study provided the evidence for the factor structure, internal consistency, reliability, and responsiveness of the 39-item “The Information Technology Scale in Nursing.” Further testing of the developed instrument with a larger number of nurses from various backgrounds and different settings is recommended.
Optical injection locking and optical-fiber data transmission by directly modulated wavelength tunable laser transmitters
Authors: Zakhleniuk N. , Duzgol O. 
Published: March 2024

Enhancement of the small- and large-signal modulation performance of wavelength tunable laser diode (TLD) transmitters under strong optical injection locking (OIL) is investigated numerically in back-to-back and optical-fiber transmission schemes. Our model is based on the spatiotemporal description of laser dynamics as due to the composite cavity design of TLDs, the usual rate equation formalism is not directly applicable. We demonstrate that TLD transmission strongly depends on wavelength tuning, which was investigated over a 21-nm range between 1529 and 1550 nm emission wavelengths. The best performance for both free-running (FR) and OIL TLDs is achieved at shorter wavelengths, 1529 nm for our device. Although in both cases this is due to larger differential material gain at shorter wavelengths, the underlying physics of the effect is completely different. For an FR TLD, it is the resonance oscillation frequency (ROF) that defines the best modulation speed, while for an OIL TLD, the achievable modulation speed depends on the cavity mode shift due to optical injection. Both the ROF and the cavity mode shift increase when the differential gain increases. However, the ROF is the device’s fixed parameter, while the cavity mode shift is defined by the OIL conditions, and thus, it can be optimized. The superior performance of the optical fiber digital data transmission with the OIL TLD is demonstrated at around 20-Gb/s modulation speed for standard fibers. This result is attributed to an enhanced modulation response and suppressed frequency chirping of the OIL TLD, and it is important for practical utilization of TLD transmitters.
Morphological and size tuning of biogenic Ag and Au nanoparticles induced by laser irradiation
Authors: Carpio-Martínez Pablo , Ruiz Víctor Fabián , Camacho-López Miguel A , Sánchez-Mendieta Víctor , Vilchis-Nestor Alfredo R 
Published: March 2024

Abstract
Since size and shape of the metal nanoparticles (NPs) determine its physical and chemical properties, such could be relevant for specific applications; it is critical to have adequate control over such features. In this work, a facile bottom-up, bio-inspired synthetic route for noble metal nanoparticles (NPs) preparation is presented. Specifically, Ag and Au monometallic nanoparticles were synthesized by bio-reduction with Citrus paradisi (Grapefruit) aqueous extract. Besides, conventional chemical reduction synthesis using NaBH4 and PVP as capping agent was also performed for comparison purposes. Characterization accomplished by UV–vis spectroscopy and electron microscopy over the synthesized nanoparticles shown that, although that unimodal (in the case of Ag NPs) or almost unimodal (Au NPs) size distributions were obtained, thanks to stabilizing effect of the ligand acting-biomolecules present in the extract; and the narrower size distribution in comparison with the conventional chemical synthesis route were observed, several morphologies were found for both metals. In order to modify such features, the obtained noble metal NPs were submitted to pulsed (30 ps, 10 mJ) Nd:YAG laser irradiation process; after that, the spherical shape seems to be the predominant morphology in both metal nanoparticles, whereas the population of particles smaller than 15 nm became increases considerably.