bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment of patients with late complications after filtering surgery
Authors: BEDNAR I , LACMANOVIC LONCAR V , KONJEVIC‐PERNAR S , MANDIC Z 
Published: October 2007

Abstract Purpose: Demonstrate the possibilities of successful surgical treatment of patients with latecomplications after filtering surgery. Methods: This study shows patients who have developed a cystically dilated or leaking filtering bleb in the late post‐surgical period after filtering surgery. Excision of the indadequate filtering bleb has been performed in the patient with a cystic bleb. After the failure of less invasive methods of treatment, we have proceeded to bleb revision with a scleral patch graft in the patient with a leaking bleb. Results: Patients treated with either bleb excision or scleral patch graft showed no signs of neither hypotonia nor secretion during the six‐months post‐surgical period. During that period, complicated cataracts developed. After cataract surgery the function of the filtering bleb was preserved. Conclusions: During the several months of patients' follow‐up, reconstructive methods of treatment have shown to be successful in high‐risk patients who have developed post‐surgical complicationsin the form of leaking or inadequate filtering bleb.
PCR identification of Rhizobium radiobacter in post‐operative endophthalmitis
Authors: VINH V , ROMANET JP , CREUZOT‐GARCHER C , BRON AM , GAIN PG , CORNUT PL , PALOMBI K , DENIS P , THURET G , CROIZE J , MAURIN M , PECHINOT A , VANDENESCH F , CHIQUET C 
Published: October 2007

Abstract Purpose: To present 2 cases of PCR identification of Rhizobium radiobacter in post‐operative endophthalmitis. Methods: Microbiological identification was carried out using samples from aqueous humor and/or vitreous. Conventional cultures were performed using a Brain Heart Infusion broth. We used broad‐range eubacterial PCR amplification followed by direct sequencing. Results: In both cases, Rhizobium radiobacter was identified using eubacterial PCR and cultures of vitreous from vitreous tap. An 81‐year‐old female presented an endophthalmitis 4 weeks after an cataract surgery. Inflammation and infection were controlled after 2 intravitreal antibiotic injections and the final visual acuity was of 20/24 at the one‐year follow‐up exam. A 75‐year‐old male who underwent a cataract surgery presented an endophthalmitis 9 days after. This patient was treated by 3 intravitreal antibiotic injections and a vitrectomy. The 6‐month follow‐up exam showed an optic nerve atrophy with a poor visual outcome (20/120). Both patients had an initial marked anterior chamber inflammation with a hypopyon and a severe retinal vasculitis was observed in the second case. Conclusions: Rhizobium radiobacter is a rare pathogen involved in postoperative endophthalmitis. As it is an environmental soil organism, we may assume that the patient's exposure to outdoor environnement and moist soil remains the source of this organism. This gram negative rod is resistant to vancomycin and have an intermediate resistance to most antibiotics used to treat post‐operative endophthalmitis. PCR allows a swifter bacterial identification than do cultures and may help choose the most efficient antibiotics.
Abnormal peripheral vascular response to occlusion provocation in normal tension glaucoma patients
Authors: WIERZBOWSKA J , STANKIEWICZ A , BURSA P , MANIEWSKI R , LIBERT A , ZBIEC A , WOJTKIEWICZ S , SIERDZINSKI J 
Published: October 2007

Abstract Purpose: To assess peripheral vascular reactive hyperemia in response to occlusion provocation test, using two‐channels laser Doppler probe in patients with normal tension glaucoma (NTG) and normal subjects. Methods: 15 patients with NTG (12 women and 4 men), mean aged 58,9 and 15 control subjects (13 women and 2 men), mean aged 60,6 were subjected to an occlusion test. The experiment comprised following steps: 1/ a 5‐minute baseline‐period 2/ a 2‐minute occlusion of the left hand using a 15 cm wide cuff located directly over the elbow (the pressure in the cuff was 50 mmHg higher than the systolic pressure measured on the arm 3/ a 15‐ minute final recovery period after occlusion. Finger hyperemia was assessed by two‐channels laser‐Doppler flowmeter MBF‐3d, Moor Instruments, Ltd., continuously during the experiment. For measurements of hyperemia two surface probes were attached to the pulp of the second finger (mean probe) and third finger (basic probe) of the left hand. The following hyperemia parameters were measured: RF (rest flow), BZ (biological zero), TM (time to peak flow), TH (half‐time of hyperemia), MAX (maximum of hyperemia) and hyperemia amplitude (MAX‐RF)/RF 100% was calculated. Kruskal‐Wallis test analysis was used to test the differences between the group of patients and normal subjects for TM1,MXF1 (basic probe) and TM2, MXF2 (mean probe) parameters. Results: In NTG patients, TM1 was significantly higher comparing with healthy subjects whereas MAX was significantly lower as compared to the control group. Conclusions: Occlusion provocation test elicits a different systemic hyperemia response in patients with NTG compared with healthy subjects.
Comparative study of commercially available antihistaminic drops in an animal model of early phase allergic conjunctivitis
Authors: MONTERO‐MORENO JA , SANCHIS‐MERINO ME , RUIZ‐MORENO JM , LUGO F , AMAT‐PERAL P , DE LA VEGA C 
Published: October 2007

Abstract Purpose: To compare the relative efficacy of topical ocular antihistaminic drops with balanced saline solution (BSS) and benzalkonium chloride (BC) in the early phase of allergic conjunctivitis in animal model. Methods: 80 male guinea pigs were sensitized with intraperitoneal egg albumin (EA) and alum. Eighteen days after sensitization the animals were topically challenged by conjunctival instillation of EA. The animals were treated 15 minutes before and 15 minutes after challenge with commercially available drugs. Ketotifen with and without preservative, olopatadine, azelastine, spaglumic acid and emedastine were tested. BSS and BC drops were tested as controls. Different drops were tested in the right and the left eye of each animal to avoid interanimal differences in allergic reactivity. The allergic reaction was measured by intravenous Evans blue extravasation (EBE) under general anaesthesia. Results: Two animals could not be used for evaluation. EBE was highest in the eyes treated by azelastine, olopatadine and preservative free ketotifen, and lowest in the eyes treated by spaglumic acid. However, when the results of the right and left eyes of each animal were considered EBE was higher in the eyes treated by BSS and BC and lowest in the eyes treated by spaglumic acid and emedastine. Conclusions: Spaglumic acid and emedastine seem to be the most useful drugs to reduce EBE in an animal model of early phase allergic conjunctivitis.
Crosstalk of Hsp70 molecular chaperone, lysosomes and proteasomes in macroautophagy‐mediated proteolysis in human retinal pigment epithelial cells
Authors: RYHÄNEN T , HYTTINEN J , KOPITZ J , MANNERMAA E , UUSITALO H , SALMINEN A , KAARNIRANTA K 
Published: October 2007

Abstract Purpose: Pathogenesis of AMD involves chronic oxidative stress, impaired degradation of membranous discs and accumulation of lysosomal lipofuscin in retinal pigment epithelial (RPE) cells. It has been estimated that a major part of cellular proteolysis occurs however in proteasomes. Prior to the proteolysis heat shock proteins tend to refold misfolded proteins and thus prevent the accumulation of protein aggregates. Connection of Hsp70, lysosomes and proteasomes were evaluated in the protein aggregation process in human RPE cells (ARPE‐19). Methods: Cellular localization of Hsp70 was studied by immunofluorescense. Confocal and transmission electron microscopy were used to detect cellular organelles and morphological changes in ARPE‐19 cells. Cell viability was analyzed by MTT ‐assay. The modulations of Hsp70 levels were done by using siRNA and overexpression vector techniques. Results: Proteasome inhibitor caused formation of juxtanuclear protein aggregates. It also caused robust accumulation of Hsp70 protein which colocalized with the protein aggregates. The granulation of aggregates was clearly increased in response to proteasome inhibition and simultaneous hsp70 mRNA interference. We found that protein aggregation is an interim, a cease of proteasome inhibition led to autophagosome‐mediated cleaning of cytoplasmic protein aggregates. The Hsp70 colocalization was observed in both primary lysosomal fractions and in autophagosomes. Conclusions: This study reveals that Hsp70, lysosomes and protesomes are connected each other in macroautophagy ‐mediated proteolysis in human RPE cells.
Grading for progression of Age Related Macular Degeneration in the London AMD Study
Authors: PETO T , SALLO FS , DANDEKAR S , LEUNG I , XING W , BUNCE C , BIRD AC 
Published: October 2007

Abstract Purpose: The aim of this study was to assess interobserver variability when grading progression of Age Related Macular Degeneration (AMD) using the International Classification (IC) system and to assess agreement with clinical decision. Methods: Fifty eyes with a minimum of 5 years disease duration documented by colour photographs were selected from the London AMD Study. Changes related to early and late AMD were graded in a random fashion while masked to the main purpose of the study (FS) and then independently by another grader (IL). Images in time‐sequence were independently analysed for clinical changes. Digitized time‐sequential fundus images were then aligned in uniform fashion, arranged into multi‐layered composites using Adobe Photoshop and clinical assessment made (TP, ACB). Results were compared and a consensus decision was made. Results: Inter‐observer agreement was 89.4% for predominant phenotype (κ=0.84), 89.36%‐91.49% for CNV (κ=0.79‐0.83), 87.23%‐89.36% for GA (κ=0.62‐0.74) and 55.32% for area covered by drusen (κ=0.31). In 4 cases the clinical and IC classifications differed: drusen area in 2 and two of end‐stage disease grading; resulting in 97.8% concordance in identification of change. At baseline,34 eyes had soft drusen, 15 had CNV and one had GA. Of the 34 with Drusen, 14 developed CNV and 9 GA by the end of the study. Disappearance of drusen without end‐stage disease was recorded in two eyes. Conclusions: Overall, changes observed clinically were reflected accurately by the IC grading in the majority of cases. This provides a quantitative basis for using this classification system in longitudinal studies. The accidental observation of regressing drusen without end‐stage disease merits further investigation.
Effect of mitomycin C on epithelial hyperplasia after laser‐assisted subepithelial keratectomy (LASEK)
Authors: TEUS MA , DE BENITO‐LLOPIS L , ORTEGA M , EGUREN M , FERRER B 
Published: October 2007

Abstract Purpose: To study the effects of mitomycin C (MMC) on the corneal regrowth after laser‐assisted subepithelial keratectomy (LASEK). Methods: We performed a prospective, observer‐masked study of 64 consecutive eyes (64 patients) scheduled to undergo LASEK to correct myopia. The patients were divided into two age‐matched groups. 32 eyes in which the ablation depth was ≤50 μm were included in group 1 and received no MMC. 32 eyes in which the ablation depth exceeded 50 μm were included in group 2 and were treated with intraoperative MMC 0.02% for 30 seconds over the ablated zone. A masked observer measured the corneal thickness 1 and 3 months postoperatively. We compared the increase in central corneal thickness (CCT) during the postoperative period between both groups, assuming that that increment would correspond mainly to the epithelial hyperplasia that occurs after surface ablation. Results: The mean patient age was 31.5±4.6 years and 31.6±8.7 years in groups 1 and 2, respectively (P=0.9). Group 1 showed a mean CCT of 444.0±41.3 μm one month after surgery and 450.3±43.5 μm three months after surgery (P=0.04). CCT in group 2 was 399.7±31.2 μm one month and 407.9±32.6 μm three months after surgery (P=0.006). The difference between the CCT increments between both groups was not statistically significant (P=0.6). Conclusions: A single intraoperative application of MMC 0.02% during 30 seconds after laser surface ablation did not seem to cause a substantial change in the postoperative corneal thickening after LASEK.
Glaucoma progression with GDx‐VCC
Authors: TOTH M , HOLLO G 
Published: October 2007

Abstract Purpose: To analyse the stability or progression of glaucomatous damage with scanning laser polarimetry (GDx‐VCC) in a longitudinal prospective study. Methods: 57 glaucoma patients and 54 healthy volunteers were examined with GDx‐VCC and standard automatic perimetry (SAP) in every 6 months. Stability or progression was assessed according to the standard visual field progression criteria. The relationship between the change of the retinal nerve fibre layer thickness (RNFLT) and the elapsed time was examined with linear regression analysis. The baseline SAP parameters were compared with unpaired t‐test. For each individual, the data of one eye were used. Results: The follow‐up time varied between 18 and 32 months. SAP progression was detectable on 17 glaucomatous eyes. The baseline visual field mean defects of the eyes with stable and progressing glaucoma did not differ significantly (7.144 dB and 9.375 dB; p=0.33). On eyes with progression, a significant negative relationship was detected between Superior Average, Superior Maximum and Maximal Modulation, and time (p<0.05). On healthy eyes and glaucomatous eyes with stable visual field, the time had no effect on the RNFLT parameters (p>0.05). Conclusions: The GDx‐VCC may be suitable for detecting mild glaucomatous progression.
Strong expresssion of matrix metalloproteinases in the melted corneas of rheumatoid arthritis patients
Authors: JUKLOVA K , JIRSOVA K 
Published: October 2007

Abstract Purpose: To compare the expression and activity of matrix metalloproteinases (MMP) 1, 2, 3, 7, 8 and 9 in human corneal grafts obtained during penetrating keratoplasty for corneal melting with their expression in normal human corneas. Methods: Ten control corneal buttons and six melted corneas were used. All patients suffered from rheumatoid arthritis (RA); in three of them RA was associated with Sjögren´s syndrome, while two suffered from peripheral ulcerative keratitis and one from keratoconjunctivitis sicca. MMP 1, 2, 3, 7, 8 and 9 were detected using indirect enzyme immunohistochemistry. The activities of MMP 2 and 9 were examined in 10 control and 4 melted specimens by gelatin zymography. Results: Weak staining for MMP 1 and 2 and very weak staining for MMP 8 were detected in normal corneal epithelium. Moderate MMP 1, 2 and 9 staining and weak staining for MMP 3, 7 and 8 were observed in corneal epithelial fragments and in the anterior stroma of all melted corneas. Weak staining for MMP 1 and 2 was detected in the posterior stroma of the melted corneas. A strong presence of the zymogen and a weak presence of the active form of MMP 2 were detected in all control corneas. Only the active form of MMP 2 was observed in three of four melted corneas, while in one of them both forms of this enzyme were present. The presence of the zymogen and the active form of MMP‐9 was extremely strong in all tested melted corneas. Conclusions: The markedly increased expression of MMP 1, 2 and 9 and the presence of the active forms of MMP 2 and 9 in melted corneal grafts suggest that these enzymes may be responsible for the destruction of the extracellular matrix seen in grafts undergoing corneal melting.
FDT Matrix 24‐2 threshold strategy for the evaluation of glaucoma suspects and ocular hypertensive eyes
Authors: MICHELONE L , TROVARELLI S , MADONIA M , DA POZZO S , DI LAURO MT , SORCE G , RAVALICO G 
Published: October 2007

Abstract Purpose: To evaluate FDT Matrix 24‐2 threshold strategy diagnostic performance in discriminating healthy subjects from glaucoma suspects (GS) and ocular hypertensives (OH) patients. Methods: 23 healthy subjects, 27 OH, 21 GS and 20 EG patients were recruited. Classification was based on optic disc and Humphrey Field Analyzer (HFA) perimetry criteria (glaucoma if PSD<95% and/or GHT outside normal limits). A complete ophthalmologic evaluation, HFA (24‐2, SITA standard) and FDT perimetry (24‐2, ZEST strategy) were performed in all subjects. A severity score (from 1 for points with <5% probability up to 4 for probability <0.5%) was calculated from defective points on pattern deviation map and mean values (±SD) in the 4 groups were compared at Mann‐Whitney test. AUROC curves were created in order to assess MD, PSD and severity score diagnostic accuracy. Results: MD and PSD displayed chance discriminating power in OHs (AUROC<0.6) on both instruments. For GSs, FDT‐MD and PSD have larger but not statistically significant AUROC than HFA. For EG, diagnostic performance of the two instruments is very similar with excellent AUROC (>0.9). Severity score on the 2 instruments are similar for OHs, whereas significance limit was nearly reached (p=0.057) for GSs and significant difference appeared (p=0.002) for EG eyes with higher score on FDT. Conclusions: FDT Matrix 24‐2 threshold strategy apparently does not offer advantages over HFA in OH and EG. In GS, statistically significant improvement in diagnostic accuracy was not demonstrated, probably because of insufficient sample size. Thus, the role of FDT Matrix in clinical practice needs further investigation.
Changes in corneal surface aberrations after myopic INTRALASIK and LASEK: Comparison between Technolas and Esiris
Authors: CANADAS SUAREZ P , ARRANZ E , GARCIA‐RECIO C , VILLAFRANCA M , TEUS MA 
Published: October 2007

Abstract Purpose: To compare high order corneal aberrations (HOCA) after myopic INTRALASIK and LASEK, using two different excimer lasers Methods: This prospective study included 237 eyes of consecutive patients divided into 4 groups; group 1 LASEK with Technolas (LT) 61 eyes, group 2 LASEK with ESIRIS (LE) 73 eyes, group 3 INTRALASIK with Technolas (IT) 61 eyes, group 4 INTRALASIK with Esiris (IE) 42 eyes.Uncorrected visual acuity (UCVA), best corrected visual acuity (BCVA) and manifest refraction were measured, in groups, before and 3 months after surgeries.The topography data were used to calculate HOCA with 6 mm pupil size, before and 3 months after surgery. Results: There were no significant differences concerning visual results 3 months after surgery in none of the 4 groups.However, there was an increase in total RMS and high order in patients treated with Esiris laser. The aberrations with INTRALASIK (p< 0,01) were higher. With Technolas laser, there were significant differences in total RMS, spherical and coma aberrations. In this case, aberrations after LASEK (p< 0,01) were higher. Conclusions: Our results suggest that both excimer lasers produce an increase in HOCA with equal visual results. Higer HOCA were found after INTRALASIK with Esiris and LASEK with Technolas.
Antimicrobial treatment of presumed ocular tuberculosis
Authors: KOUPRIANOFF S , CHIQUET C , BOUILLET L , ROMANET JP 
Published: October 2007

Abstract Purpose: Uveitis secondary to a tuberculosis is rarely reported, even in a tertiary a care center. The prevalence of tuberculosis is low in Western Europe and its microbiological identification is difficult. However, anti tuberculosis treatment may be useful when the diagnosis of tuberculosis is presumed. Methods: The clinical records of patients with suspected tuberculosis uveitis referred to the Ophthalmology Department of the Grenoble University, between January 2005 and January 2007 were retrospectively analyzed. Patients were included in this series if they received a specific antituberculosis treatment. Results: This series included 10 patients (3M/7F, mean age 54.1). The clinical features of ocular inflammation were: bilateral panuveitis, episcleritis, bilateral posterior uveitis, and pars planitis. Tuberculin skin test, chest computerized tomography, BK sputum, and internal medicine consultation were performed for all patients. The diagnosis of presumed tuberculosis was based upon: history, thoracic imaging, and tuberculin skin test. None had extra‐ocular symptoms. Sputum cultures were negative, 2 adenopathy biopsies confirmed the diagnosis. Nine patients received specific antituberculosis therapy, without systemic steroid therapy. All of them improved; no relapse occurred after 1 to 2 years after the end of therapy. In one case, tuberculosis specific therapy allowed to taper the systemic steroid therapy. Conclusions: The diagnosis of uveitis associated with tuberculosis is difficult since it depends on a spectrum of indirect signs. Bacteriological identification is rarely obtained. In presumed ocular tuberculosis, antituberculosis therapy may be useful to control intraocular inflammation, with or without steroid therapy.
Diagnostic coincidence of the technologies of analysis of image HRT, OCT and GDx‐VCC
Authors: ALIAS EG , BORQUE E , LARROSA JM , POLO V , FERRERAS A , HONRUBIA FM 
Published: October 2007

Abstract Purpose: To value the diagnostic coincidence and specificity between different structural parameters of the topography papilar realized with the HRT3, the optic coherence tomography Stratus OCT 3000 and the laser polarimetry GDx‐VCC. Methods: There were included 41 eyes of 41 normal subjects ( intraocular pressure <21mmHg, normal optic nerve head morphology and standard automated perimetry ), and, 312 eyes of 312 consecutive patients sent by the ophtalmologist of zone for suspicion to be able to suffer glaucoma. An analysis was realized to each of them by means of HRT, OCT and GDx‐VCC and there was valued the diagnostic opposing coincidence. Considered to be criteria of damage a Moorfields Regresión Analysis ‐ MRA ‐> 95 % and a Glaucoma Probability Score ‐ GPS ‐> 0.51 to level of the HRT, a NFI> 30 in the GDx and an average thickness or in a quadrant of the cap of retinal nerve fiver layer thickness with a value of p <5 % by OCT. Results: The specificities of MRA and GPS were 85 % and 80 %, in the GDx‐VCC of 78 % and in the OCT of 71 %. Between the suspects they found 113 positive cases by means of MRA, 153 by GPS, 101 by OCT and 64 by GDx‐VCC. Conclusions: It is necessary to possess the analyzers of image in the screening of the glaucoma since they contribute a high specificity, being major the contributed one for the HRT. The opposing specificity is not associated with a low number of positive cases and the diagnostic coincidence of the different analyzers is high.Key words: glaucoma, diagnosis, HRT, OCT, GDx‐VCC.