bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Changing Landscape of Neonatal Diabetes Mellitus in Italy Between 2003 and 2022
Authors: Rapini Novella , Delvecchio Maurizio , Mucciolo Mafalda , Ruta Rosario , Rabbone Ivana , Cherubini Valentino , Zucchini Stefano , Cianfarani Stefano , Prandi Elena , Schiaffini Riccardo , Bizzarri Carla , Piccini Barbara , Maltoni Giulio , Predieri Barbara , Minuto Nicola , Di Paola Rossella , Giordano Mara , Tinto Nadia , Grasso Valeria , Russo Lucia , Tiberi Valentina , Scaramuzza Andrea , Frontino Giulio , Maggio Maria Cristina , Musolino Gianluca , Piccinno Elvira , Tinti Davide , Carrera Paola , Mozzillo Enza , Cappa Marco , Iafusco Dario , Bonfanti Riccardo , Novelli Antonio , Barbetti Fabrizio ,   Diabetes Study Group of Italian Society for Pediatric Endocrinology and Diabetes (ISPED), Beccaria Luciano , Candia Francesco , Cauvin Vittoria , Cardani Roberta , Cardella Francesca , Favia Anna , Gallo Francesco , Garzia Patrizia , Ghirri Paolo , Innaurato Stefania , Iughetti Lorenzo , Laforgia Nicola , Lo Presti Donatella , Marsciani Alberto , Meschi Franco , Panzeca Rossana , Pasquino Bruno , Pesavento Roberta , Pezzino Giulia , Reinstadler Petra , Ripoli Carlo , Savastio Silvia , Timpanaro Tiziana , Tumini Stefano , Vento Gianni 
Published: February 2024

Abstract

Context
In the last decade the Sanger method of DNA sequencing has been replaced by next-generation sequencing (NGS). NGS is valuable in conditions characterized by high genetic heterogeneity such as neonatal diabetes mellitus (NDM).

Objective
To compare results of genetic analysis of patients with NDM and congenital severe insulin resistance (c.SIR) identified in Italy in 2003-2012 (Sanger) vs 2013-2022 (NGS).

Methods
We reviewed clinical and genetic records of 104 cases with diabetes onset before 6 months of age (NDM + c.SIR) of the Italian dataset.

Results
Fifty-five patients (50 NDM + 5 c.SIR) were identified during 2003-2012 and 49 (46 NDM + 3 c.SIR) in 2013-2022. Twenty-year incidence was 1:103 340 (NDM) and 1:1 240 082 (c.SIR) live births. Frequent NDM/c.SIR genetic defects (KCNJ11, INS, ABCC8, 6q24, INSR) were detected in 41 and 34 probands during 2003-2012 and 2013-2022, respectively. We identified a pathogenic variant in rare genes in a single proband (GATA4) (1/42 or 2.4%) during 2003-2012 and in 8 infants (RFX6, PDX1, GATA6, HNF1B, FOXP3, IL2RA, LRBA, BSCL2) during 2013-2022 (8/42 or 19%, P = .034 vs 2003-2012). Notably, among rare genes 5 were recessive. Swift and accurate genetic diagnosis led to appropriate treatment: patients with autoimmune NDM (FOXP3, IL2RA, LRBA) were subjected to bone marrow transplant; patients with pancreas agenesis/hypoplasia (RFX6, PDX1) were supplemented with pancreatic enzymes, and the individual with lipodystrophy caused by BSCL2 was started on metreleptin.

Conclusion
NGS substantially improved diagnosis and precision therapy of monogenic forms of neonatal diabetes and c.SIR in Italy.
Biograficzność w oświacie dorosłych – wybrane stanowiska
Authors: Dubas Elżbieta 
Published: February 2024

W artykule przedstawiono rodowód metody biograficznej i rozwój paradygmatu biograficzności w andragogice. Wiodącym pojęciem biograficzności w naukach pedagogicznych jest „biografia edukacyjna”. Uwidacznia się ona w poglądach np. Gastona Pineau, Pierre’a Dominicé, Horsta Sieberta, Dietera Nittela, Petera Alheita, które zostały zaprezentowane w artykule. Szczególną uwagę poświęcono dorobkowi Olgi Czerniawskiej i jej wkładowi w budowanie i rozwój koncepcji biograficzności w Polsce. Artykuł wieńczy refleksja na temat czynności i postawy badacza biografii
OS BASTIDORES DE UMA PESQUISA DURANTE A PANDEMIA: UMA TRIOETNOGRAFIA COM DIÁLOGOS COLABORATIVOS
Authors: Paniago Maria Cristina Lima , Moura Gustavo , Arguelho Miriam 
Published: February 2024

Este artigo surge a partir do interesse dos autores em entender os diálogos colaborativos estabelecidos nos bastidores de uma pesquisa guarda-chuva da qual fazem parte. Trata-se de uma pesquisa qualitativa que envolve pesquisadores (PIBIC, professores universitários, e pós-graduandos) de três países - Brasil, Canadá e Inglaterra, com foco na cultura digital em comunidades educativas em programas de Pós-Graduação Stricto Sensu. A abordagem metodológica deste artigo parte de uma perspectiva trioetnográfica cujo estilo de escrita acadêmica é mais abstrato, personalizado e conversacional. A partir dos dados emergentes, exploramos 3 (três) momentos em particular: a criticidade, o neoliberalismo, e o COVID-19 na realidade docente e suas implicações no desenrolar desta pesquisa; o papel da competência linguística e da tradução na problematização do que consideramos como práticas democráticas e colaborativas; e a renegociação (intercultural) do desenvolvimento da pesquisa. Estes momentos são interpretados pelos autores a partir de suas vivências e experiências, justapostas neste artigo para refletir a complexidade de se produzir uma pesquisa sob esta perspectiva dialógica e colaborativa em um contexto de pandemia. Os diálogos neste artigo aprofundam discussões emergentes numa perspectiva crítico-reflexiva trazendo à tona percepções, olhares, sentimentos e implicações das inter-relações pessoais, afetivas, profissionais e acadêmico-científicas no contexto de desenvolvimento desta pesquisa. Entendemos, a partir dos dados produzidos, de maneira rigorosa mas inconclusa, que nossas perspectivas analíticas, carregadas por diversas concepções, contextos e histórias de vida, possuem aproximações e distanciamentos, enriquecendo este processo de investigação.
Mangrove gastropod distribution based on dominant vegetation classes and their relationship with physicochemical characteristics on fringe mangroves of Lembongan Island, Bali, Indonesia
Authors: ERNAWATI NI MADE , DEWI AYU PUTU WIWEKA KRISNA , SUGIANA I PUTU , DHARMAWAN I WAYAN EKA , MA'RUF MIFTACHUDIN SYAIFUL , GALGANI GEMMA ANTONIA 
Published: February 2024

Abstract. Ernawati NM, Dewi APWK, Sugiana IP, Dharmawan IWE, Ma’ruf MS, Galgani GA. 2024. Mangrove gastropod distribution based on dominant vegetation classes and their relationship with physicochemical characteristics on fringe mangroves of Lembongan Island, Bali, Indonesia. Biodiversitas 25: 156-166. Mangrove ecosystems are important in supporting diverse marine life, offering refuge, reproductive grounds, and sustenance to various species. However, mangroves possess distinct zoning patterns determined by dominant species due to environmental variations that significantly impact the biodiversity of biota, particularly gastropods. A study on the Indonesian island of Nusa Lembongan revealed distinct communities of coexisting gastropod species in each mangrove vegetation zone. We discovered 29 distinct species of mangrove gastropods, with the Sonneratia mangrove zone having the most and the Lumnitzera zone having the least. Certain species, including Amphidromus perversus, Cerithium adustum, and Echinolittorina sundaica, were detected in only one of the mangrove zones. In each mangrove zone, the composition of gastropod species changed depending on the substrate type, e.g., soil, mangrove trunks, or mangrove leaves. The Shannon-Wiener diversity index values range from low to high diversity, and the community structure index values in mangrove zones vary. The evenness index varied by region, with Avicennia, Bruguiera, and Sonneratia having the highest index value. The findings indicate that mangrove vegetation zones are essential for maintaining diverse gastropod populations, with distinct composition and abundance patterns influenced by the distance of each mangrove zone from the ocean. This data may influence conservation efforts to preserve mangrove habitats and the diverse array of organisms that depend on them.
Detecting single nanoparticles using fiber-tip nanophotonics
Authors: Hendriks Arthur L. , Rabelink Daan , Dolci Mathias , Dreverman Paco , Cano-Velázquez Mildred S. , Picelli Luca , van Veldhoven René P. J. , Zijlstra Peter , Verhagen Ewold , Fiore Andrea 
Published: February 2024

Sensing nano-objects, from nanoparticles to molecules, has become a crucial need in environmental monitoring, medical diagnostics, and drug development. Detection of single particles and molecules is highly desirable, as it provides specific information on size, dynamics, and interactions. Current nanophotonic implementations rely on complex optical readout schemes, limiting their application in the field. Here we demonstrate a nanophotonic fiber-tip sensor with a compact sensor footprint and a simple readout scheme. We leverage advanced design methods to simultaneously achieve a small mode volume V
m
=0.74(λ/n)3, narrow linewidth Δλ=0.4nm, and a large modulation ΔR≈20% in reflection from the fiber. This unique combination of properties opens the way to sensing weak nanoscale perturbations in the vicinity of the fiber tip. In particular, we experimentally demonstrate the real-time detection of single 50 nm nanoparticles. This opens a route towards real-time sensing of single nanoparticles, and potentially single molecules, in environmental monitoring and diagnostics.
Body image perception and physical activity behavior among adult population: Application of trans-theoretical model of behavior change
Authors: Mahdifar Mozhgan , Tavakoly Sany Seyedeh Belin , Tehrani Hadi , Ghavami Vahid , Vahedian Shahroodi Mohammad 
Published: February 2024

Background
The human body changes during life, but research on how sociodemographic characteristics and physical activity (PA) related to all aspects of body image are still unclear and there is conflict in the relevant results. This study aims to examine gender-specific body image perception and physical activity in relation to BMI and sociodemographic characteristics among health employees who worked in health and medical care centers.

Methods
The study was a population-based cross-sectional survey of 170 health employees (55 men and 115 women) in Binaloud, Iran. Participants completed a self-administered questionnaire, which consisted of a self-administered Multidimensional Body-Self Image Questionnaire (MBSRQ) and stages of change scales. The full version of Marcus-TTM based on the stages of physical activity changes was used based on 5 items related to regular physical activity behavior and intentions.

Results
There were no significant differences between men and women in total body image score, body area satisfaction, and attitude. Disease orientation (p = 0.02) and health orientation (p = 0.05) were the only significant differences between men and women. The number of children, level of education, BMI, and PA had a stronger influence on body image concerns and body dissatisfaction. PA and 7 of the 10 subclasses of MBSRQ (appearance evaluation, fitness evaluation, fitness orientation, health evaluation, health orientation, and illness orientation) showed a significant difference (P<0.05) between participants in the five TTM stages.

Conclusion
The ideal body image and body satisfaction may differ by the number of children, level of education, gender, BMI, and PA. Evidence from this study supports that participants’ stages of change affect their physical activity and body image perception. This result suggests a need for conducting work/home place intervention to promote adults’ body image perception and body area satisfaction based on the usage-specific stage under consideration.
Are Radiomic Spleen Features Useful for Assessing the Response to Infliximab in Patients With Crohn's Disease? A Multicenter Study
Authors: Tang Chao-Tao , Yin Fang , Yin Yitian , Liu Zide , Long Shunhua , Zeng Chun-Yan , Chen Yong , Chen You-Xiang 
Published: February 2024

INTRODUCTION:
To develop and validate a radiomics nomogram for assessing the response of patients with Crohn's disease (CD) to infliximab.

METHODS:
Radiomics features of the spleen were extracted from computed tomography enterography images of each patient's arterial phase. The feature selection process was performed using the least absolute shrinkage and selection operator algorithm, and a radiomics score was calculated based on the radiomics signature formula. Subsequently, the radiomic model and the clinical risk factor model were separately established based on the radiomics score and clinically significant features, respectively. The performance of both models was evaluated using receiver operating characteristic curves, decision curve analysis curves, and clinical impact curves.

RESULTS:
Among the 175 patients with CD, 105 exhibited a clinical response, and 60 exhibited clinical remission after receiving infliximab treatment. Our radiomic model, comprising 20 relevant features, demonstrated excellent predictive performance. The radiomic nomogram for predicting clinical response showed good calibration and discrimination in the training cohort (area under the curve [AUC] 0.909, 95% confidence interval [CI] 0.840–0.978), the validation cohort (AUC 0.954, 95% CI 0.889–1), and the external cohort (AUC = 0.902, 95% CI 0.83–0.974). Accordingly, the nomogram was also suitable for predicting clinical remission. Decision curve analysis and clinical impact curves highlighted the clinical utility of our nomogram.

DISCUSSION:
Our radiomics nomogram is a noninvasive predictive tool constructed from radiomic features of the spleen. It also demonstrated good predictive accuracy in evaluating CD patients' response to infliximab treatment. Multicenter validation provided high-level evidence for its clinical application.
Psychometric Properties of the Draw a Story (DAS) Test in Depressed and Normal Children
Authors: Ashtari R. , Khormaei F. , Rahimi C. , Mohammadi N. 
Published: February 2024

Objective: understanding the problems of depressed children is important, as they may express their issues through painting and storytelling, which can provide insight into their excitability and self-image. This study investigated the psychometric properties of the Draw a Story test in children with depression and normal ones.Material and methods. The participants of this study included 60 fourth and fifth grade students in Yazd province, who were divided into two groups; depressed and normal. Then, each group was evaluated by drawing a story test (DAS).Results. The results showed that there was a significant difference between the depressed and normal groups in terms of self-image and emotion content, as determined by a two-way analysis of variance. The normal group significantly demonstrated a stronger self-image and emotional state than the depressed group. There was no significant difference between boys and girls in terms of self-image. However, there was a significant difference in their emotional expression, with girls being more adept at expressing their emotions compared to boys. Convergent validity of the DAS was established by correlating it with the DSRS test for self-image and emotion, resulting in correlations of -0.67 and -0.74, respectively. The reliability of the DAS test was assessed using the test-retest method for self-image and emotion, yielding coefficients of 0.82, 0.90, respectively. Additionally, the raters' agreement coefficient was calculated for self-image and emotion, and was 0.68 and 0.82, respectively.Conclusions. The results of this study revealed the reliability and validity of the test (DAS) in the group of depressed and normal children. This test can be used to diagnose children with depression.
Evaluation of the therapeutic efficacy of the drug Cytochrome C in the treatment of asthenia in outpatients (CITRIN study)
Authors: Lobzin V. Yu. , Emelin A. Yu. , Kolmakova K. A. 
Published: February 2024

Asthenic syndrome occurs in most chronic diseases, and doctors encounter it in outpatient appointments for both somatic and neurological pathologies. Drugs that normalize energy metabolism, have an antioxidant effect and antihypoxic properties are used to treat asthenia.Objective: to investigate the therapeutic efficacy of the drug Cytochrome C in patients with asthenia of various origin in an outpatient setting.Material and methods. The study included 147 outpatients with asthenia who received Cytochrome C 10 mg intramuscularly daily for 10 days. The assessment of the initial level of asthenia manifestations and their changes in dynamics was carried out using the MFI-20 scales, the assessment of daytime sleepiness, the assessment of fatigue (FAS), the severity of symptoms of the disease and the dynamics of the condition according to the doctor and the patient.Results. By the 10th day of therapy, there was a decrease in the severity of asthenia on the MFI-20 scale from 72.2±12.7 to 41.1±12.3 points (p<0.01), including general (by 45%), physical (by 42%) and mental (by 45%) asthenia according to the MFI-20 subscales (p<0.001), improvement in night sleep and reduction in daytime sleepiness on the Epworth scale by 65% – from 9.1±5.6 to 4.6±4.3 points (p<0.001), reduction in severity of fatigue by 29% (p<0.01). The clinically significant improvement lasted until the 30th day of observation, 3 weeks after the end of the therapy. Overall, a significant reduction in asthenia symptoms was observed in 99% of patients on day 10 of therapy and in 96% of cases on day 30.Conclusion. Cytochrome C has been shown to be very effective in patients with asthenia as it reduces all manifestations of asthenia, improves night sleep and reduces daytime sleepiness.
Lassen Sie uns über das Scheitern bei Projekten mit kommerziellen Partnern sprechen: Ein Interview mit Andreas Degkwitz
Authors: Degkwitz Andreas 
Published: February 2024

Zusammenfassung
Die Deutsche Forschungsgemeinschaft (DFG) und der Wissenschaftsrats (WR) haben 2011 den sechs deutschen Bibliotheksverbünden empfohlen, sich mit ihren Infrastrukturen auf zeitgemäße, überregionale Services auszurichten. Für den Aufbau einer „Cloudbasierten Infrastruktur für Bibliotheksdaten“ (CIB) erhielten ab 2013 die Verbünde BVB, HEBIS und KOBV im Rahmen einer Förderausschreibung den Zuschlag. In dem Interview werden Herausforderungen, Probleme und das Scheitern dieses Vorhabens aus Sicht des Interviewten erörtert. Dabei stellen sich allerdings auch Aspekte heraus, die sich für solche Vorhaben künftig zur Berücksichtigung empfehlen.
An isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS)-based candidate reference measurement procedure (RMP) for the quantification of phenobarbital in human serum and plasma
Authors: Schierscher Tobias , Salzmann Linda , Singh Neeraj , Bachmann Martina , Kobel Anja , Wild Janik , Bauland Friederike , Geistanger Andrea , Risch Lorenz , Geletneky Christian , Seger Christoph , Taibon Judith 
Published: February 2024

Abstract

Objectives
Phenobarbital serves as an antiepileptic drug (AED) and finds application in the treatment of epilepsy either as monotherapy or adjunctive therapy. This drug exhibits various pharmacodynamic properties that account for its beneficial effects as well as potential side effects. Accurate measurement of its concentration is critical for optimizing AED therapy through appropriate dose adjustments. Therefore, our objective was to develop and validate a new reference measurement procedure (RMP) for the accurate quantification of phenobarbital levels in human serum and plasma.

Methods
A sample preparation protocol based on protein precipitation followed by a high dilution step was established in combination with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using a C8 column to separate target analytes from known and unknown interferences. Assay validation and determination of measurement uncertainty were performed based on current guidelines. Selectivity and Specificity were assessed using spiked serum and plasma samples; to investigate possible matrix effects (MEs) a post-column infusion experiment and a comparison of standard line slopes was performed. Precision and accuracy were determined within a multiday precision experiment.

Results
The RMP was shown to be highly selective and specific, with no evidence of matrix interferences. It can be used to quantify phenobarbital in the range of 1.92 to 72.0 μg/mL. Intermediate precision was less than 3.2 %, and repeatability coefficient of variation (CV) ranged from 1.3 to 2.0 % across all concentration levels. The relative mean bias ranged from −3.0 to −0.7 % for native serum levels, and from −2.8 to 0.8 % for Li-heparin plasma levels. The measurement uncertainties (k=1) for single measurements and target value assignment were 1.9 to 3.3 % and 0.9 to 1.6 %, respectively.

Conclusions
A novel LC-MS/MS-based candidate RMP for the quantification of phenobarbital in human serum and plasma is presented which can be used for the standardization of routine assays and the evaluation of clinically relevant samples.
Does EU Regulation Adequately Address the Tension between CCPs Shareholders’ and Clearing Members’ Incentives?
Authors: Sotiropoulou Anastasia 
Published: February 2024

Abstract

964The current EU regulatory regime of Central Counterparties (CCPs) falls short of addressing adequately the potential misalignment of incentives of CCPs’ shareholders on the one hand and clearing members on the other hand.Thus, according to the European Market Infrastructure Regulation (EMIR), while clearing members contribute substantially to the default waterfall of a CCP, they do not enjoy substantial governance rights: they merely participate in the risk committee of the CCP, whose role is only advisory.By contrast, although shareholders are vested with substantial governance rights, such as the right to appoint the members of the board which sets the CCP’s risk profile, they do not bear final losses first, as in ordinary companies: the shareholders’ contribution to a CCP’s default waterfall is limited and, in case the CCP enters resolution, they only bear losses, in principle, after the clearing members.It is however clear that when the owners of a firm are not the ones bearing the risks first, the firm may be inclined to excessive risk-taking. The objective of this article is therefore to discuss a number of ways to improve the corporate governance of CCPs, in particular the incentive setting for shareholders.965
Browning formation markers of subcutaneous adipose tissue in relation to resting energy expenditure, physical activity and diet in humans
Authors: Dinas Petros C. , Valente Angelica , Granzotto Marnie , Rossato Marco , Vettor Roberto , Zacharopoulou Aikaterini , Carrillo Andres E. , Davies Natalie A. , Gkiata Paraskevi , Jamurtas Athanasios Z. , Koutedakis Yiannis , Metsios George S. , Flouris Andreas D. 
Published: February 2024

Abstract

Background
Regular exercise and diet may contribute to white adipose tissue (WAT) conversion into a brown adipose-like phenotype that may increase resting energy expenditure (REE), leading to weight loss. We examined the relationship between REE, physical activity (PA) participation and diet with browning formation markers of subcutaneous WAT in healthy men.

Materials and methods
We assessed REE, diet and body composition of 32 healthy men [age (years): 36.06 ± 7.36, body mass index (BMI): 27.06 ± 4.62 (kg/m2)]. Participants also underwent measurements of PA [metabolic equivalent (MET)-min/week] using the International Physical Activity Questionnaire (IPAQ), while they undertook a subcutaneous fat biopsy from the abdominal region to assess the mRNA expressions of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), peroxisome proliferator-activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma (PPARγ).

Results
We found no associations between the UCP1, PGC-1α, PPARα and PPARγ mRNAs with REE, PA levels and diet (p > 0.05). However, the PGC-1α, PPARα and PPARγ mRNAs were more expressed in individuals displaying moderate rather than low PA levels (p < 0.05). Furthermore, PGC-1α, PPARα and PPARγ mRNAs were negatively correlated with fat mass percentage (p < 0.05). PGC-1α and PPARα mRNAs were also negatively correlated with BMI, while PGC-1α mRNA was inversely associated with waist-to-hip ratio (p < 0.05).

Conclusion
REE, PA levels and diet are not associated with browning formation indices of subcutaneous adipose tissue in healthy adult men.