bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate Variability and Sugarcane Yield in Louisiana
Authors: Greenland David 
Published: December 2005

Abstract
This paper seeks to understand the role that climate variability has on annual yield of sugarcane in Louisiana. Unique features of sugarcane growth in Louisiana and nonclimatic, yield-influencing factors make this goal an interesting and challenging one. Several methods of seeking and establishing the relations between yield and climate variables are employed. First, yield–climate relations were investigated at a single research station where crop variety and growing conditions could be held constant and yield relations could be established between a predominant older crop variety and a newer one. Interviews with crop experts and a literature survey were used to identify potential climatic factors that control yield. A statistical analysis was performed using statewide yield data from the American Sugar Cane League from 1963 to 2002 and a climate database. Yield values for later years were adjusted downward to form an adjusted yield dataset. The climate database was principally constructed from daily and monthly values of maximum and minimum temperature and daily and monthly total precipitation for six cooperative weather-reporting stations representative of the area of sugarcane production. The influence of 74 different, though not independent, climate-related variables on sugarcane yield was investigated. The fact that a climate signal exists is demonstrated by comparing mean values of the climate variables corresponding to the upper and lower third of adjusted yield values. Most of these mean-value differences show an intuitively plausible difference between the high- and low-yield years. The difference between means of the climate variables for years corresponding to the upper and lower third of annual yield values for 13 of the variables is statistically significant at or above the 90% level. A correlation matrix was used to identify the variables that had the largest influence on annual yield. Four variables [called here critical climatic variables (CCV)], mean maximum August temperature, mean minimum February temperature, soil water surplus between April and September, and occurrence of autumn (fall) hurricanes, were built into a model to simulate adjusted yield values. The CCV model simulates the yield value with an rmse of 5.1 t ha−1. The mean of the adjusted yield data over the study period was 60.4 t ha−1, with values for the highest and lowest years being 73.1 and 50.6 t ha−1, respectively, and a standard deviation of 5.9 t ha−1. Presumably because of the almost constant high water table and soil water availability, higher precipitation totals, which are inversely related to radiation and temperature, tend to have a negative effect on the yields. Past trends in the values of critical climatic variables and general projections of future climate suggest that, with respect to the climatic environment and as long as land drainage is continued and maintained, future levels of sugarcane yield will rise in Louisiana.
The Characteristics of the Chicago Lake Breeze and Its Effects on Trace Particle Transport: Results from an Episodic Event Simulation
Authors: Harris Lucas , Kotamarthi V. Rao 
Published: December 2005

Abstract
The lake-breeze circulation that forms over Lake Michigan during the summer influences the Chicago, Illinois, metropolitan area’s weather in several ways. Of particular significance is the circulation’s effect on the dispersion of pollutants such as ozone and aerosols produced in and around the city. To investigate these effects, the fifth-generation Pennsylvania State University–National Center for Atmospheric Research Mesoscale Model (MM5) was used to perform numerical simulations for two lake-breeze events—one in July 1999 and another in July 2002. The model runs were verified with data from several locations around the Chicago area. The simulated breeze circulation decreased the rate of increase in air temperature while penetrating roughly 12 km inland and lasting about 8 h, in reasonable agreement with observations. Furthermore, the inland penetration distance was related to the strength of the maximum vertical velocity within the front. Calculations of trajectories and transport of particles showed that the breeze tended to transport particles trapped within it to the north when release occurred before the circulation came ashore, whereas particles released at the time of the breeze’s landfall or afterward moved more northeasterly, in the direction of the prevailing wind. Thirty-four percent of all released particles were trapped by the circulation and raised to a height of at least 300 m, and 20% of the particles remained in the lowest 100 m above the surface. In addition, sensitivity tests showed little change in the modeled breeze when measured surface temperatures for Lake Michigan were used as initial conditions and boundary conditions in the place of surface skin temperature (as derived by the National Centers for Environmental Prediction). Raising the lake temperatures significantly in the simulation yielded a more elongated vertical circulation and a briefer lake-breeze event that did not reach as far inland.
Rainfall Estimation in the Sahel. Part I: Error Function
Authors: Ali Abdou , Lebel Thierry , Amani Abou 
Published: December 2005

AbstractRainfall estimation in semiarid regions remains a challenging issue because it displays great spatial and temporal variability and networks available for monitoring are often of low density. This is especially the case in the Sahel, a region of 3 million km2 where the life of populations is still heavily dependent on rain for agriculture. Whatever the data and sensors available for rainfall estimation—including satellite IR and microwave data and possibly weather radar systems—it is necessary to define objective error functions to be used in comparing various rainfall products. This first of two papers presents a theoretical framework for the development of such an error function and the optimization of its parameters for the Sahel. A range of time scales—from rain event to annual—are considered, using two datasets covering two different spatial scales. The mesoscale [Estimation des Pluies par Satellite (EPSAT)-Niger (E-N)] is documented over a period of 13 yr (1990–2002) on an area of 16 000 km2 covered by 30 recording rain gauges; the regional scale is documented by the Centre Regional Agrometeorologie–Hydrologie–Meteorologie (AGRHYMET) (CRA) dataset, with an annual average of between 600 and 650 rain gauges available over a period of 8 yr. The data analysis showed that the spatial structure of the Sahelian rain fields is markedly anisotropic, nonstationary, and dominated by the nesting of two elementary structures. A cross-validation procedure on point rainfall values leads to the identification of an optimal interpolation algorithm. Using the error variances computed from this algorithm on 1° × 1° and 2.5° × 2.5° cells, an error function is derived, allowing the calculation of standard errors of estimation for the region. Typical standard errors for monthly rainfall estimation are 11% (10%) for a 10-station network on a 2.5° × 2.5° (1° × 1°) grid, and 40% (30%) for a single station on a 2.5° × 2.5° (1° × 1°) grid. In a companion paper, this error function is used to investigate the differences between satellite rainfall products and how they compare with ground-based estimates.
Rainfall Estimation in the Sahel. Part II: Evaluation of Rain Gauge Networks in the CILSS Countries and Objective Intercomparison of Rainfall Products
Authors: Ali Abdou , Amani Abou , Diedhiou Arona , Lebel Thierry 
Published: December 2005

Abstract
This study investigates the accuracy of various precipitation products for the Sahel. A first set of products is made of three ground-based precipitation estimates elaborated regionally from the gauge data collected by Centre Regional Agrometeorologie–Hydrologie–Meteorologie (AGRHYMET). The second set is made of four global products elaborated by various international data centers. The comparison between these two sets covers the period of 1986–2000. The evaluation of the entire operational network of the Sahelian countries indicates that on average the monthly estimation error for the July–September period is around 12% at a spatial scale of 2.5° × 2.5°. The estimation error increases from south to north and remains below 10% for the area south of 15°N and west of 11°E (representing 42% of the region studied). In the southern Sahel (south of 15°N), the rain gauge density needs to be at least 10 gauges per 2.5° × 2.5° grid cell for a monthly error of less than 10%. In the northern Sahel, this density increases to more than 20 gauges because of the large intermittency of rainfall. In contrast, for other continental regions outside Africa, some authors have found that only five gauges per 2.5° × 2.5° grid cell are needed to give a monthly error of less than 10%. The global products considered in this comparison are the Climate Prediction Center (CPC) merged analysis of precipitation (CMAP), Global Precipitation Climatology Project (GPCP), Global Precipitation Climatology Center (GPCC), and Geostationary Operational Environmental Satellite (GOES) precipitation index (GPI). Several methods (scatterplots, distribution comparisons, root-mean-square error, bias, Nash index, significance test for the mean, variance, and distribution function, and the standard deviation approach for the kriging interval) are first used for the intercomparison. All of these methods lead to the same conclusion that CMAP is slightly the better product overall, followed by GPCC, GPCP, and GPI, with large errors for GPI. However, based on the root-mean-square error, it is found that the regional rainfall product obtained from the synoptic network is better than the four global products. Based on the error function developed in a companion paper, an approach is proposed to take into account the uncertainty resulting from the fact that the reference values are not the real ground truth. This method was applied to the most densely sampled region in the Sahel and led to a significant decrease of the raw evaluation errors. The reevaluated error is independent of the gauge references.
Ambient Sulfate Trends and the Influence of Meteorology
Authors: Mueller Stephen F. 
Published: December 2005

Abstract
Data on atmospheric levels of sulfur dioxide (SO2) and sulfate were examined to quantify changes since 1989. Changes in sulfur species were adjusted to account for meteorological variability. Adjustments were made using meteorological variables expressed in terms of their principal components that were used as predictors in statistical models. Several models were tested. A generalized additive model (GAM)—based in part on nonparametric, locally smoothed predictor functions—computed the greatest association between sulfate and the meteorological predictors. Sulfate trends estimated after a GAM-based adjustment for weather-related influences were found to be primarily downward across the eastern United States by as much as 6.7% per year (average of −2.6% per year), but large spatial variability was noted. The most conspicuous characteristic in the trends was over portions of the Appalachian Mountains where very small (average = −1.6% per year) and often insignificant sulfate changes were found. The Appalachian region also experienced a tendency, after removing meteorological influences, for increases in the ratio RS of sulfate sulfur to total sulfur. Before 1991, this ratio averaged 0.33 across all sites. Appalachian increases in RS were equivalent to 0.07 during 1989–2001 (significant for most sites at the 0.05 level), or nearly 2 times the average change at the other sites. This suggests that conditions over the Appalachians became notably more efficient at oxidizing SO2 into sulfate. Alternatively, subtle changes in local deposition patterns occurred, preferentially in and near mountainous monitoring sites, that changed the SO2–sulfate balance.
The Notch ligand delta-1 is a hematopoietic development cofactor for plasmacytoid dendritic cells
Authors: Olivier Aurélie , Lauret Evelyne , Gonin Patrick , Galy Anne 
Published: December 2005

AbstractPlasmacytoid dendritic cells (pDCs) play an important role in innate and adaptive immunity, prompting interest in mechanisms controlling the production of this lineage of cells. Notch signaling via one of the Notch ligands, delta-like 1 (delta-1), influences the hematopoietic development of several lymphoid and myeloid lineages, but whether or not delta-1 affects the formation of pDCs is unknown and was tested here. Human CD34+ progenitor cells were cultured onto delta-1–expressing OP9 stroma in the presence of flt-3 ligand and IL-7, and this efficiently generated BDCA-2+ CD123+ CD4+ CD11c– cells with the characteristic morphology of pDCs, expressing toll-like receptor-9 (TLR9), pre-Tα mRNAs, and secreting CpG-induced IFN-α. Delta-1 augmented the numbers of BDCA-2+ cells produced without affecting their proliferation, and the effect was blocked by γ-secretase inhibition. The development of pDCs was stroma-, delta-1–, and cytokine-dependent and could be induced from committed lymphoid progenitor cells, which responded to delta-1 by opposite changes in pDC- and B-cell production. Our results identify delta-1 as a novel factor enhancing pDC hematopoiesis and delineate a new role for Notch signaling in lymphopoiesis by showing its opposite effect on pDC and B lineage determination.
Human mast cells express multiple EP receptors for prostaglandin E2 that differentially modulate activation responses
Authors: Feng Chunli , Beller Elizabeth M. , Bagga Savita , Boyce Joshua A. 
Published: December 2005

AbstractProstaglandin E2 (PGE2) blocks mast-cell (MC)-dependent allergic responses in humans but activates MCs in vitro. We assessed the functions of the EP receptors for PGE2 on cultured human MCs (hMCs). hMCs expressed the EP3, EP2, and EP4 receptors. PGE2 stimulated the accumulation of cyclic adenosine monophosphate (cAMP), and suppressed both FcϵRI-mediated eicosanoid production and tumor necrosis factor-α (TNF-α) generation. PGE2 also caused phosphorylation of extracellular signal-regulated kinase (ERK), exocytosis, and production of prostaglandin D2 (PGD2), as well as leukotriene C4 (LTC4) when protein kinase A (PKA) was inhibited. An EP3 receptor-selective agonist, AE-248, mimicked PGE2-mediated ERK phosphorylation, exocytosis, and eicosanoid formation. Selective agonists of both EP2 and EP4 receptors (AE1-259-01 and AE-329, respectively) stimulated cAMP accumulation. No selective agonist, alone or in combination, was as effective as PGE2. AE-248, AE1-259-01, and AE-329 all inhibited FcϵRI-mediated TNF-α generation, while AE1-259-01 blocked eicosanoid production. PGE2 caused the expression of inducible cAMP early repressor (ICER) by a pathway involving PKA and ERK. Thus, while PGE2 activates MCs through EP3 receptors, it also counteracts FcϵRI-mediated eicosanoid production through EP2 receptors and PKA, and blocks cytokine transcription. These functions explain the potency of PGE2 as a suppressor of early- and late-phase allergic responses.
Novel stabilin-1 interacting chitinase-like protein (SI-CLP) is up-regulated in alternatively activated macrophages and secreted via lysosomal pathway
Authors: Kzhyshkowska Julia , Mamidi Srinivas , Gratchev Alexei , Kremmer Elisabeth , Schmuttermaier Christina , Krusell Liis , Haus Georg , Utikal Jochen , Schledzewski Kai , Scholtze Joachim , Goerdt Sergij 
Published: December 2005

AbstractMammalian Glyco_18-domain–containing proteins include catalytically active chitinases and chitinase-like proteins with cytokine activity involved in host defense and Th2-type inflammatory reactions. Here, we describe a novel human Glyco_18-domain–containing protein, SI-CLP, as an interacting partner of the endocytic/sorting receptor stabilin-1. Similarly to the chitinase-like cytokines YKL-39, YKL-40, and YM1/2, SI-CLP lacks a chitin-binding domain and catalytic amino acids. Using a novel mAb 1C11, we demonstrated that SI-CLP is sorted into late endosomes and secretory lysosomes in human alternatively activated macrophages. The direct interaction of SI-CLP with stabilin-1, their colocalization in the trans-Golgi network, and the reduced sorting of SI-CLP into lysosomes in macrophages treated with stabilin-1 siRNA suggest that stabilin-1 is involved in intracellular sorting of SI-CLP. Expression of SI-CLP in macrophages was strongly up-regulated by the Th2 cytokine IL-4 and by dexamethasone. This effect was suppressed by IFNγ but not affected by IL-10. In contrast, expression of YKL-40 was induced by IFNγ and suppressed by dexamethasone. Macrophages treated with IL-4 secreted SI-CLP, while costimulation with dexamethasone blocked secretion and resulted in intracellular accumulation of SI-CLP. The 1C11 mAb detected SI-CLP in human bronchoalveolar lavage and peripheral-blood leukocytes (PBLs), and can be used to analyze the role of SI-CLP in human disorders.
The small GTPase Rap1b regulates the cross talk between platelet integrin α2β1 and integrin αIIbβ3
Authors: Bernardi Bruno , Guidetti Gianni F. , Campus Francesca , Crittenden Jill R. , Graybiel Ann M. , Balduini Cesare , Torti Mauro 
Published: December 2005

AbstractThe involvement of the small GTPase Rap1b in platelet integrin α2β1-dependent outside-in signaling was investigated. Platelet adhesion to 4 different specific ligands for integrin α2β1, monomeric collagen, decorin, and collagen-derived peptides CB8(II) and CB11(II), induced a robust and rapid activation of Rap1b. This process did not require secreted ADP or thromboxane A2 production but was critically regulated by phospholipase C (PLC)–derived second messengers. Both Ca2+ and protein kinase C were found to organize independent but additive pathways for Rap1b activation downstream of integrin-α2β1, which were completely blocked by inhibition of PLC with U73122. Moreover, integrin α2β1 engagement failed to trigger Rap1b activation in murine platelets lacking CalDAG-GEFI, a guanine nucleotide exchange factor regulated by Ca2+ and diacylglycerol, despite normal phosphorylation and activation of PLCγ2. In addition, CalDAG-GEFI–deficient platelets showed defective integrin α2β1-dependent adhesion and spreading. We found that outside-in signaling through integrin α2β1 triggered inside-out activation of integrin αIIbβ3 and promoted fibrinogen binding. Similarly to Rap1b stimulation, this process occurred downstream of PLC activation and was dramatically impaired in murine platelets lacking the Rap1 exchange factor CalDAG-GEFI. These results demonstrate that Rap1b is an important element in integrin-dependent outside-in signaling during platelet adhesion and regulates the cross talk between adhesive receptors.
Cigarette smoke-induced pulmonary emphysema in scid-mice. Is the acquired immune system required?
Authors: D'hulst An I , Maes Tania , Bracke Ken R , Demedts Ingel K , Tournoy Kurt G , Joos Guy F , Brusselle Guy G 
Published: December 2005

AbstractBackgroundChronic obstructive pulmonary disease is associated with a chronic inflammatory response of the host to chronic exposure to inhaled toxic gases and particles. Although inflammatory cells of both the innate and adaptive immune system infiltrate the lungs in pulmonary emphysema and form lymphoid follicles around the small airways, the exact role of the acquired immune system in the pathogenesis of emphysema is not known.MethodsIn this study, wild typeBalb/cmice and immunodeficientscidmice – which lack functional B- and T-cells – were exposed to mainstream cigarette smoke (CS) for 5 weeks or 6 months.ResultsSubacute CS-exposure for 5 weeks significantly increased innate inflammatory cells (neutrophils, macrophages and dendritic cells) in the bronchoalveolar lavage (BAL) fluid of wild type mice andscidmice, which correlated with the CS-induced upregulation of the chemokines Monocyte Chemotactic Protein-1, Macrophage Inflammatory Protein-3α and KC (= mouse Interleukin-8). Chronic CS-exposure for 6 months significantly increased the number of neutrophils, macrophages, dendritic cells, CD4+and CD8+T-lymphocytes in BAL fluid and lungs of wild type mice compared to air-exposed littermates, and augmented the size and number of peribronchial lymphoid follicles. In contrast, neither B-lymphocytes, nor T-lymphocytes, nor lymphoid follicles could be discerned in the lungs of air- or CS-exposedscidmice. Importantly, chronic CS-exposure induced pulmonary emphysema in both wild type animals andscidmice, as evidenced by a significant increase in the mean linear intercept and the destructive index of CS-exposed versus air-exposed animals. The CS-induced emphysema was associated with increased mRNA expression of matrix metalloproteinase-12 in the lungs and increased protein levels of Tumor Necrosis Factor-α in the BAL fluid of CS-exposedBalb/candscidmice compared to air-exposed littermates.ConclusionThis study suggests that the adaptive immune system is not required per se to develop pulmonary emphysema in response to chronic CS-exposure, since emphysema can be induced inscidmice, which lack lymphoid follicles as well as functional B- and T-cells.
Dimerization and enzymatic activity of fungal 17β-hydroxysteroid dehydrogenase from the short-chain dehydrogenase/reductase superfamily
Authors: Kristan Katja , Deluca Dominga , Adamski Jerzy , Stojan Jure , Rižner Tea Lanišnik 
Published: December 2005

Abstract

Background
17β-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus (17β-HSDcl) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily. SDR proteins usually function as dimers or tetramers and 17β-HSDcl is also a homodimer under native conditions.

Results
We have investigated here which secondary structure elements are involved in the dimerization of 17β-HSDcl and examined the importance of dimerization for the enzyme activity. Sequence similarity with trihydroxynaphthalene reductase from Magnaporthe grisea indicated that Arg129 and His111 from the αE-helices interact with the Asp121, Glu117 and Asp187 residues from the αE and αF-helices of the neighbouring subunit. The Arg129Asp and His111Leu mutations both rendered 17β-HSDcl monomeric, while the mutant 17β-HSDcl-His111Ala was dimeric. Circular dichroism spectroscopy analysis confirmed the conservation of the secondary structure in both monomers. The three mutant proteins all bound coenzyme, as shown by fluorescence quenching in the presence of NADP+, but both monomers showed no enzymatic activity.

Conclusion
We have shown by site-directed mutagenesis and structure/function analysis that 17β-HSDcl dimerization involves the αE and αF helices of both subunits. Neighbouring subunits are connected through hydrophobic interactions, H-bonds and salt bridges involving amino acid residues His111 and Arg129. Since the substitutions of these two amino acid residues lead to inactive monomers with conserved secondary structure, we suggest dimerization is a prerequisite for catalysis. A detailed understanding of this dimerization could lead to the development of compounds that will specifically prevent dimerization, thereby serving as a new type of inhibitor.