bluuebunny/crossref_metadata_2025_split huggingface dataset:
Busulfan Produces Efficient Human Cell Engraftment in NOD/LtSz-Scid IL2Rγ
Null Mice
Authors: Hayakawa Jun , Hsieh Matthew M. , Uchida Naoya , Phang Oswald , Tisdale John F. 
Published: October 2008

Abstract
Xenografting immunodeficient mice after low-dose irradiation has been used as a surrogate human hematopoietic stem cell (HSC) assay; however, irradiation requires strict and meticulous animal support and can produce significant mortality rates, limiting the usefulness of this model. In this work, we examined the use of parenteral busulfan as an alternative conditioning agent. Busulfan led to dose-dependent human HSC engraftment in NOD/LtSz-scid/IL2Rγnull mice, with marked improvement in survival rates. Terminally differentiated B and T lymphocytes made up most of the human CD45+ cells observed during the initial 5 weeks post-transplant when unselected cord blood (CB) products were infused, suggesting derivation from existing mature elements rather than HSCs. Beyond 5 weeks, CD34+-enriched products produced and sustained superior engraftment rates compared with unselected grafts (CB CD34+, 65.8% ± 5.35%, vs. whole CB, 4.27% ± 0.67%, at 24 weeks). CB CD34+ group achieved significantly higher levels of engraftment than mobilized CD34+-enriched peripheral blood (PB CD34+). At 8 weeks, all leukocyte subsets were detected, yet human red blood cells (RBCs) were not observed. Transfused human red cells persisted in the chimeric mice for up to 3 days; an accompanying rise in total bilirubin suggested hemolysis as a contributing factor to their clearance. Recipient mouse-derived human HSCs had the capacity to form erythroid colonies in vitro at various time points post-transplant in the presence of human transferrin (Tf). When human Tf was administered singly or in combination with anti-CD122 antibody and human cytokines, up to 0.1% human RBCs were detectable in the peripheral blood. This long evasive model should prove valuable for the study of human erythroid cells.
Assessing the risks of aquatic species invasions via european inland waterways: from concepts to environmental indicators
Authors: Panov Vadim E , Alexandrov Boris , Arbačiauskas Kęstutis , Binimelis Rosa , Copp Gordon H , Grabowski Michal , Lucy Frances , Leuven Rob SEW , Nehring Stefan , Paunović Momir , Semenchenko Vitaliy , Son Mikhail O 
Published: October 2008

Abstract
Over the past century, the potential for aquatic species to expand their ranges in Europe has been enhanced both as a result of the construction of new canals and because of increased international trade. A complex network of inland waterways now connects some previously isolated catchments in southern (Caspian, Azov, Black, Mediterranean seas) and northern (Baltic, North, Wadden, White seas) Europe, and these waterways act as corridors for nonnative species invasions. We have developed a conceptual risk assessment model for invasive alien species introductions via European inland waterways, with specific protocols that focus on the development of environmental indicators within the socioeconomic context of the driving forces-pressures-state-impact-response framework. The risk assessment protocols and water quality indicators on alien species were tested for selected ecosystems within 3 main European invasion corridors, and these can be recommended for application as part of the Common Implementation Strategy of the European Commission Water Framework Directive, which aims to provide a holistic risk-based management of European river basins. The conceptual structure of the online Risk Assessment Toolkit for aquatic invasive alien species is provided and includes 3 main interlinked components: online risk assessment protocols, an early warning system, and an information transmitter for risk communication to end users.
Assessing the effects of hydromorphological degradation on macroinvertebrate indicators in rivers: examples, constraints, and outlook
Authors: Friberg Nikolai , Sandin Leonard , Pedersen Morten L 
Published: October 2008

Abstract
An extensive amount of literature on linkages between the in-stream physical environment and river benthic macroinvertebrates reports a number of relationships across multiple spatial scales. We analyzed data on different spatial scales to elucidate the linkages between different measurements of hydromorphological degradation and commonly used macroinvertebrate indices. A regression analysis of 1049 sites from 3 countries revealed that the strongest relationship between a biotic metric—average score per taxon—and physiochemical variables (R2 = 0.61) was obtained with a multiple regression model that included concentration of total phosphorus and percent arable land in the catchment, as well as hydromorphological quality variables. Analyses of 3 data sets from streams primarily affected by hydromorphological degradation showed an overall weak relationship (max R2 = 0.25) with the River Habitat Survey data of 28 Swedish streams, whereas moderate (R2 ≈︁ 0.43) relationships with more detailed measurements of morphology were found in 2 Danish studies (39 and 6 streams, respectively). Although evidence exists in the literature on the importance of physical features for in-stream biota in general and macroinvertebrates specifically, we found only relatively weak relationships between various measures of hydromorphological stress and commonly used macroinvertebrate assessment tools. We attribute this to a combination of factors, including 1) the mixed nature of pressures acting on the majority of river reaches, 2) scaling issues (spatial and temporal) when relating habitat surveys to macroinvertebrate assessments, and 3) the scope of commonly used macroinvertebrate assessment systems (mainly focusing on water chemistry perturbation, such as eutrophication and acidification). The need is urgent to develop refined and updated biological assessment systems targeting hydromorphological stress for the use of the European Water Framework Directive (WFD) and national water-related policies.
Late Jurassic continental flood basalt doleritic dykes in northwestern Cuba: remnants of the Gulf of Mexico opening
Authors: Allibon James , Lapierre Henriette , Bussy François , Tardy Marc , Cruz Gàmez Esther M. , Senebier François 
Published: October 2008

Abstract
Accreted terranes, comprising a wide variety of Late Jurassic and Early Cretaceous igneous and sedimentary rocks are an important feature of Cuban geology. Their characterization is helpful for understanding Caribbean paleo-geography.
The Guaniguanico terrane (western Cuba) is formed by upper Jurassic platform sediments intruded by microgranular dolerite dykes. The geochemical characteristics of the dolerite whole rock samples and their minerals (augitic clinopyroxene, labradorite and andesine) are consistent with a tholeiitic affinity. Major and trace element concentrations as well as Nd, Sr and Pb isotopes show that these rocks also have a continental affinity. Sample chemistry indicates that these lavas are similar to a low Ti-P2O5 (LTi) variety of continental flood basalts (CFB) similar to the dolerites of Ferrar (Tasmania). They derived from mixing of a lithospheric mantle source and an asthenopheric component similar to E-MORB with minor markers of crustal contamination and sediment assimilation. However, the small quantity of Cuban magmatic rocks, similarly to Tasmania, Antarctica and Siberia differs from other volumetrically important CFB occurrences such as Parana and Deccan.
These dolerites are dated as 165-150 Ma and were emplaced during the separation of the Yucatan block from South America. They could in fact be part of the Yucatan-South America margin through which the intrusive system was emplaced and which was later accreted to the Cretaceous arc of central Cuba and to the Palaeogene arc of eastern Cuba. These samples could therefore reflect the pre-rift stage between North and South America and the opening of the gulf of Mexico.
The Evaluation of the Healing Rate of Subtalar Arthrodeses, Part 2: The Effect of Low-Intensity Ultrasound Stimulation
Authors: Coughlin Michael J. , Smith Bertil W. , Traughber Paul 
Published: October 2008

Background: Arthrodeses of hindfoot joints is commonly used to treat a multitude of painful conditions and deformity. Use of adjuvant low-intensity ultrasound bone stimulation has demonstrated promising results in the treatment of acute fractures and fracture nonunions. The purpose of this 12-month prospective study was to evaluate the healing rate and clinical results of patients undergoing primary subtalar arthrodeses with adjuvant low-intensity ultrasound bone stimulation. Materials and Methods: Fifteen consecutive patients participated in the study. Routine radiographs and CT scans were obtained, and clinical outcomes gathered. The clinical and radiographic data were compared to a similar cohort of patients previously reported on that had not received ultrasound bone stimulation. Results: The patients who received ultrasound bone stimulation showed a statistically significant faster healing rate on plain radiographs at 9 weeks ( p = 0.034) and CT scan at 12 weeks ( p = 0.017). A 100% fusion rate was noted. The American Orthopaedic Foot and Ankle Society (AOFAS) ankle and hindfoot score was also improved at 12 months postoperatively, a finding that was statistically significant ( p = 0.026). Conclusion: This is the first paper, to our knowledge, to prospectively evaluate ultrasound bone stimulation in primary hindfoot arthrodesis patients. We were able to show significantly improved radiographic as well as clinical outcomes compared with a similar cohort of patients who did not receive adjuvant ultrasound stimulation. We believe that low-intensity ultrasound bone stimulation is indicated in primary hindfoot fusions, particularly in those patients at higher risk for nonunion. Level of Evidence: II, Prospective Comparative Study
Sports and Recreation Activity of Varus and Valgus Ankle Osteoarthritis before and after Realignment Surgery
Authors: Pagenstert Geert , Leumann André , Hintermann Beat , Valderrabano Victor 
Published: October 2008

Background: Realignment-surgery to unload ankle osteoarthritis (OA) has been proposed as treatment alternative for varus and valgus ankle OA. Sports activity after this procedure has not been analyzed. Realignment-surgery increases sports activity. Sports activity correlates with ankle pain, function, and alignment, but does not influence revision rate. Materials and Methods: Prospective case series of 35 consecutive patients with post-traumatic varus or valgus ankle OA limited to half tibiotalar joint surface were treated by OA unloading realignment-surgery. Distal tibia osteotomy was used in all cases; additional osteotomies, tendon, ligament procedures in 92% of cases. Main Outcome Measurements: Pain (visual-analogue-scale; VAS), ankle range-of-motion (ROM); function (American-Orthopaedic-Foot-and-Ankle-Society (AOFAS) ankle-score; Swiss-symptom-related-Ankle-Activity-Scale (SAAS); Sports-Frequency-Score (SFS), OA and tibiotalar-alignment-grade (Takakura-Score), and revision surgery. Mean followup was 5 years. Results: Mean values from preoperative to followup: VAS decreased ( p = 0.0001) 4 points; ankle ROM increased ( p = 0.001) 5 degrees; AOFAS-Score increased ( p = 0.0001) 46 points; SAAS increased ( p = 0.0001) 42 points; SFS increased ( p = 0.02) 0.5 grades; Takakura-score decreased ( p = 0.0001) 1.0 grades. Revision surgery was performed in 10 cases (29%). Three of these were revised to ankle arthroplasty. At follow-up, SAAS correlated with VAS, AOFAS score, Takakura score, and not with ROM or SFS. SFS did not correlate with other variables. Patients needing revision surgery had a higher ( p = 0.003) SFS than patients who needed no revision. Conclusion: Realignment-surgery increased sports activity of ankle OA patients. Improved ankle pain and function correlated with ability to perform activity without symptoms; however, sports frequency had no correlation to patients' symptoms but showed higher revision rate. Level of Evidence: II, Prospective Comparative Study
Tibiocalcaneal Arthrodesis Using the Ilizarov Technique in the Presence of Bone Loss and Infection of the Talus
Authors: Rochman Robert , Hutson James Jackson , Alade Oladapo 
Published: October 2008

Background: Infected nonunions and extrusions of the talus can often lead to below-knee amputation. Limb-salvage procedures have goals of eradicating infection and creating a painless, stable limb. Often, a tibiocalcaneal fusion is the best option; however, in the presence of infection and bone loss, it can be difficult to achieve a successful outcome using internal fixation. We review the results of circular ring external fixation to obtain a tibiocalcaneal arthrodesis despite these obstacles. Methods: A retrospective review of 11 patients who underwent tibiocalcaneal arthodesis using an Ilizarov external fixator for infected talar nonunions or extrusions was performed. Each patient had a debridement of all nonviable talus. The bony surfaces were prepared for the fusion followed by application of a circular ring fixator. Clinical outcomes were measured using the AOFAS ankle-hindfoot scale. There was a mean followup of 35 months. Results: Nine of the 11 patients had successful fusions. One fused successfully after a revision and the other developed a stable pseudoarthrosis. Eight patients underwent concomitant lengthening with the Ilizarov fixator. Mean AOFAS score at final followup was 65. This was out of a maximum of 86 since the tibiotalar and subtalar joint motion were removed. There were no recurrent deep infections or amputations. Conclusions: Tibiocalcaneal arthrodesis using the Ilizarov technique is a viable alternative to amputation in patients with infected nonunions or large bone loss of the talus. Level of Evidence: IV, Retrospective Case Study
Lateral Talar Process Fracture and Peroneal Tendon Dislocation: A Previously Unrecognized Injury Complex
Authors: Klein Sandra E. , Varner Kevin E. , Marymont John V. 
Published: October 2008

Background: Lateral talar process fractures and peroneal tendon dislocations are frequently unrecognized at the time of injury. Lateral process fractures were initially classified by Hawkins as three types. Type II injuries are comminuted fractures involving both the talofibular and talocalcaneal articular surfaces. The purpose of this retrospective chart review was to describe an injury complex of Type II lateral talar process fracture with peroneal tendon dislocation. Materials and Methods: Between January of 1995 and December 2006, 13 patients were seen for a lateral talar process fracture. Patients' charts were reviewed for fracture classification, mechanism of injury, radiographic studies, treatment, secondary procedures, length of followup and return to previous activity level. Concurrent peroneal tendon dislocations were identified in a subset of these patients. Results: Thirteen patients were identified with lateral talar process fractures all of which were classified as a Hawkins Type II. Six patients (46%) had a simultaneous peroneal tendon dislocation. All patients underwent operative excision of the comminuted lateral process. Patients with the injury complex were more likely to undergo additional operative procedures, and were more likely to develop subtalar arthritis. At final followup, 71% of patients with isolated lateral process fractures and 33% of injury complex patients had returned to their previous level of activity Conclusion: An injury complex of Hawkins Type II lateral talar process fractures and peroneal tendon dislocation exists. Patients with comminuted lateral talar process fractures, especially those resulting from high-energy injuries, should be carefully evaluated for the possibility of concurrent peroneal tendon dislocation. Level of Evidence: IV, Retrospective Case Study
Proximal Spherical Metatarsal Osteotomy for the Foot with Severe Hallux Valgus
Authors: Tanaka Yasuhito , Takakura Yoshinori , Kumai Tsukasa , Sugimoto Kazuya , Taniguchi Akira , Hattori Koji 
Published: October 2008

Background: A proximal spherical metatarsal osteotomy was devised to correct not only varus deviation of the first metatarsal, but also dorsiflexion. We expected to increase the medial longitudinal arch by adding plantar flexion at the osteotomy site. To investigate the limitations of this procedure for feet with severe hallux valgus, a followup study was performed on patients with preoperative hallux valgus angles greater than or equal to 40 degrees. Materials and Methods: Forty-eight feet in 37 patients (10 male, 27 female) (60 years; range, 20 to 84 years) were investigated. Mean followup was 4 years and 1 month, ranging from 2 to 8 years. The spherical osteotomy was performed using a curved chisel. A distal soft tissue procedure was done at the same time. Twenty feet received combined operations for their combined deformities. Results: While 81% of patients were satisfied with the results, 50% of those with preoperative hallux valgus angles greater than or equal to 50 degrees had postoperative hallux valgus angle greater than or equal to 20 degrees. In these patients, correction of metatarsus primus varus was good, but correction of valgus deviation of the hallux was fair. Mean correction toward plantar flexion was 1.5 degrees and no increase in arch height was achieved. Conclusion: The proximal spherical osteotomy could consistently achieve satisfactory results for the patients with hallux valgus angles less than 50 degrees. However, the corrections were worse in feet with more severe deformities. Furthermore, plantarflexion at the osteotomy site was offset by displacement at the first tarsometatarsal joint. If plantarflexion is indispensable, arthrodesis of the tarsometatarsal joint is recommended. Level of Evidence: IV, Retrospective Case Series
Peripheral intracranial aneurysms: management challenges in 60 consecutive cases
Authors: Nussbaum Eric S. , Madison Michael T. , Goddard James K. , Lassig Jeffrey P. , Nussbaum Leslie A. 
Published: October 2008

Object
The authors report the management and outcomes of 55 patients with 60 intracranial aneurysms arising distal to the major branch points of the circle of Willis and vertebrobasilar system.

Methods
Between July 1997 and December 2006, the authors' neurovascular service treated 2021 intracranial aneurysms in 1850 patients. The database was reviewed retrospectively to identify peripherally located intracranial aneurysms. Aneurysms that were mycotic and aneurysms that were associated with either an arteriovenous malformation or an atrial myxoma were excluded from review.

Results
The authors encountered 60 peripheral intracranial aneurysms in 55 patients. There were 42 small, 7 large, and 11 giant lesions. Forty-one (68%) were unruptured, and 19 (32%) had bled. Fifty-three aneurysms were treated surgically by using direct clip reconstruction in 26, trapping or proximal occlusion with distal revascularization in 21, excision with end-to-end anastomosis in 3, and circumferential wrap/clip reconstruction in 3. Coils were used to treat 6 aneurysms, and 1 was treated by endovascular parent artery occlusion. Overall, 49 patients had good outcomes, 4 were left with new neurological deficits, and 2 died.

Conclusions
Peripherally situated intracranial aneurysms are rare lesions that present unique management challenges. Despite the fact that in the authors' experience these lesions were rarely treatable with simple clipping of the aneurysm neck or endovascular coil occlusion, preservation of the parent artery was possible in most cases, and the majority of patients had a good outcome.
Tethered cord syndrome: nationwide inpatient complications and outcomes
Authors: Lad Shivanand P. , Patil Chirag G. , Ho Christopher , Edwards Michael S. B. , Boakye Maxwell 
Published: October 2008

Object
Previous investigations of health outcome after spinal surgery for tethered cord syndrome (TCS) have been single-institution studies. The aim of this study was to report inpatient complications and outcomes on a nationwide level.

Methods
The Nationwide Inpatient Sample (NIS) was used to identify patients who underwent spinal surgery for TCS in the US between 1993 and 2002. Patients who had a primary diagnosis of TCS (ICD-9 742.59) and also underwent spinal laminectomies were included in this study. Multivariate analysis was performed to analyze the effects of patient and hospital characteristics on variables such as mortality rate, nonfatal complications, LOS, and adverse outcomes in general (defined as death or discharge to an institution rather than home).

Results
The NIS sample included data on 9733 patients with TCS who underwent surgery. The means for mortality rate, complication rate, and LOS, respectively, were 0.0005%, 9.48%, and 5.6 days. Postoperative hemorrhages or hematomas (mean rate 2.3%) were the most common complications reported. Age and complications were the only significant predictors of adverse outcome on multivariate analysis. Patients older than 65 years had a threefold increase in risk of adverse outcome compared with patients 18 to 44 years of age. On average, one postoperative complication led to a 3-day increase in mean LOS and added more than $9000 to hospital charges.

Conclusions
This study provides a national perspective on inpatient complications and outcomes after spinal surgery for TCS in the United States. The authors have demonstrated the impact of age, complications, and medical comorbidities on the outcome of surgery for patients with this common disorder.
Corporatisme et colonialisme : approche du cas mozambicain, 1933-1979. I. Une genèse difficile, un mouvement squelettique.
Authors: Cahen Michel 
Published: October 2008

M. Cahen — Corporatism and Colonialism : An Approach of Mocambique's Case, I933-I979, I- A Troublesome Genesis for a Skeletal Movement.
The first part of this study (to be continuee! in the next issue) raises the general problem of the specifie particulars of Portuguese colonialism, and more precisely of the possible effect on colonial society of a corporativist, fascist metropolitan State. Insofar as Portugal itself is England's 'semi-colony' while Mocambique is fully integrated within South Africa's economie System, are peculiar characte-ristics of the colony to be explained by fascism in its metropolis? A tentative answer is here based upon an analysis of the officiai, legal birth of 'colonial corporatism' (1937) and, later on (1942-1960), of the first 'national trade-unions' and gremios. A study of the actual working of these structures and of their socio-economic role leads one to consider them as operating quite outside the colonial economy and almost completely lacking any social basis for their existence. The same analysis applied to the fascist political structures (single party, youth orga-nization) reveals the regime's failure in its attempts to set up a real movement among the colonial petty bourgeoisie and the White working-class aristocracy.
De la quête de légitimation à la question de la légitimité : les thérapeutiques « traditionnelles » au Sénégal.
Authors: Fassin Éric , Fassin Didier 
Published: October 2008

E. Fassin & D. Fassin — From the Quest for Legitimation to the Question of Legitimacy : 'Traditional' Therapies in Senegal.
The quest for new forms of recognition for traditional therapists raises the question of the relations between the different kinds of medical practices and, more precisely, that of medical legitimacy. Through three case studies carried out in Senegal, the authors attempt to show how principles of legitimation are defined today with, as essential characteristics: recognition of the least legitimate of the healers, strengthening of the legitimator's authority, and recourse to legitimating bodies outside of the medical field. This analysis shows the inanity of the classical modem/traditional opposition and leads to an examination of the meaning of a legitimacy crisis which seems to affect both doctors and healers.