bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insufficient sagittal endplate-bone graft contact is a risk factor for high-grade cage subsidence occurring after lateral lumbar interbody fusion supplemented with lateral plate: An analysis of 122 cases
Authors: Chen Ruijie , Xu Zheng , Chen Qixin , Lai Oujie , Li Hao 
Published: March 2024

Abstract

Background
Lateral lumbar interbody fusion (LLIF) is a minimally invasive fusion technique that can be performed with lateral plate. Insufficient contact between the endplate and bone graft in the fusion segment may result in instability and subsequent cage subsidence. This study aimed to investigate the potential correlation between endplate-bone graft contact and high-grade cage subsidence (HCS) occurring after LLIF supplemented with lateral plate.
Method
Between June 2017 and February 2023, 122 patients (47 males, 75 females; mean age 62.7 years; minimum follow-up period 12 months) undergoing LLIF supplemented with lateral plate were retrospectively reviewed. The incidence of HCS was assessed, and patients were categorized into HCS group or non-HCS group based on the occurrence of HCS. Comparative analyses were performed on demographic characteristics, surgical variables, and parameters related to endplate-bone graft contact between the two groups. Multivariable logistic regression analysis was employed to identify the potential risk factors associated with HCS.
Results
The HCS group comprised 13 patients, while the non-HCS group included 109 patients. The incidence of HCS occurring after LLIF supplemented with lateral plate was 10.7%. The sagittal contact rate of endplate-bone graft (OR, 0.844; 95% CI, 0.766–0.931; P < 0.001) and inferior cage-endplate angle (OR, 1.869, 95% CI, 1.215–2.873, P = 0.004) were determined to be significantly correlated with HCS occurring after LLIF supplemented with lateral plate. Compared to non-HCS group, the patients in HCS group had a lower sagittal contact rate of endplate-bone graft and a larger inferior cage-endplate angle.
Conclusion
The incidence of HCS occurring after LLIF supplemented with lateral plate was 10.7%. HCS was significantly associated with insufficient sagittal endplate-bone graft contact. Further study aiming to optimize the sagittal endplate-cage contact in the procedure of LLIF supplemented with lateral plate are warranted to enhance clinical outcomes.
Exosomal-derived miR4485-3p suppresses CD40L to attenuate the function of CD4 + T cells in Mycobacterium leprae infection
Authors: gao wei , Jiang Haiqin , Wang Le , Wang Hongsheng , Meade Yumi , Qin Xihu 
Published: March 2024

Abstract
Background
The expressions of serum exosomes microRNAs (miRNAs) exhibited variations among patients with leprosy, which may have implications for disease diagnosis and treatment. Various miRNAs play crucial roles in the pathogenesis of leprosy infection.
Methods
We identified miRNAs in three groups of serum exosomes by miRNA microarray. And we expanded up to 30 samples of three groups to validate the differentially expressed miRNAs from serum exosome samples. An human mononuclear/macrophage and Mycobacterium leprae (M. leprae) co-culture system was established in vitro to confirm the expressions of significantly differentially expressed miRNA in supernatant exosomes and cells. Finally, validate the regulatory effects of differentially expressed miRNAs on their predicted target genes through in vitro cell experiments.
Results
Consequently, we isolated exosomes from the serum samples. The expression of serum exosome miR4485-3p was significantly higher in leprosy patients compared with normal controls (p < 0.01), and both were found to be elevated in multi-bacillary (MB) leprosy patients compared to pauci-bacillary (PB) leprosy patients. After conducting validation experiments, miR-4485-3p was found to be able to distinguish between healthy control individuals and leprosy patients with a sensitivity of 70% and specificity of 73.3%. After transfecting miR4485-3p mimics into monocytes/macrophages treated with Mycobacterium leprae and co-cultivating them with autologous T cells, we observed that it suppressed the function of CD4+T cells by down-regulating CD40L expression. Additionally, it also inhibited macrophage activity by down-regulating CD40 expression and decreasing the levels of TNF-α, IL-1β, and IL-6 in the supernatant, thereby promoting bacterial survival.
Conclusions
We have identified higher expression of miR4485-3p in serum exosomes from leprosy patients. Furthermore, Mycobacterium leprae-infected macrophages can modulate CD40L receptors on the surface of CD4+ T cells by delivering miR4485-3p via exosomes, thereby impairing their function in the immune response to Mycobacterium leprae infection.
Biochanin A inhibits excitotoxicity-triggered ferroptosis by targeting GPX4 in hippocampal neurons
Authors: Won Jun Pil , Lee Hyuk Gyoon , Yoon Han Jun , Seo Han Geuk 
Published: March 2024

Abstract
Excitatory neurotransmitter-induced neuronal ferroptosis has been implicated in multiple neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. Although there are several reports pertaining to the pharmacological activities of biochanin A, the effects of this isoflavone on excitotoxicity-triggered neuronal ferroptosis remain unclear. In this study, we demonstrate that biochanin A inhibits ferroptosis of mouse hippocampal neurons induced by glutamate or the glutamate analog, kainic acid. Biochanin A significantly inhibited accumulation of intracellular iron and lipid peroxidation in glutamate- or kainic acid-treated mouse hippocampal neurons. Furthermore, biochanin A regulated the level of glutathione peroxidase 4, a master regulator of ferroptosis, by modulating its autophagy-dependent degradation. We observed that biochanin A reduced the glutamate-induced accumulation of intracellular iron by regulating expression of iron metabolism-related proteins including ferroportin-1, divalent metal transferase 1, and transferrin receptor 1. Taken together, these results indicate that biochanin A effectively inhibits hippocampal neuronal death triggered by glutamate or kainic acid. Our study is the first to report that biochanin A has therapeutic potential for the treatment of diseases associated with hippocampal neuronal death, particularly ferroptosis induced by excitatory neurotransmitter.
Non-dimensional parameters for the flapping dynamics of multilayered plates
Authors: Jain Esha , Saravanakumar Aditya Karthik , Joshi V. , Gurugubelli P S 
Published: March 2024

Abstract
The coupled flow-induced flapping dynamics of flexible plates in uniform axial flows are governed by three non-dimensional numbers, namely: Reynolds number (Re), mass-ratio (m*), and non-dimensional flexural rigidity (KB). The traditional definition of these parameters is limited to isotropic single-layered flexible plates. However, there is a need to define these parameters for a more generic plate of multiple isotropic layers placed on top of each other. In this work, we derive the non-dimensional parameters for a flexible plate of n-isotropic layers and validate the non-dimensional parameters with numerical simulations. The proposed non-dimensional framework connects the experimental, numerical, and analytical studies on single-layered plates with the flapping dynamics of multilayered plates.
Solvent Effects on the Absorption and Emission Spectra of the 5ABBM molecule
Authors: Lalasangi Dayanand , Hanagodimath S. M. , Khanadal Tairabi , Padmashali Basavaraj , Jadhav Mangesh S. 
Published: March 2024

Abstract

The solvent effects on the absorption and emission spectra and dipole moments of the 5ABBM molecule have been extensively studied in a series of solvents. The dipole moments in the excited state are observed to be greater than their ground-state counterparts in all the solvents studied for the chosen molecule. The dipole moment increase in the excited singlet state ranges from 2.42 to 24.14 D. The various methods for a correct prediction of solvatochromic shifts are recalled with reference to previous conflicting theoretical interpretations using Lippert’s, Bakhshiev's, and Kawski-Chamma-Viallet’s equations. Experimentally calculated ground state and excited state dipole moments were calculated using the solvatochromatic shifts of absorption and emission spectra as a function of the dielectric constant (ɛ) and refractive index (n). These data are used to estimate the excited-state dipole moment using an experimentally determined ground-state dipole moment. A series of fifteen different organic solvents (toluene, methanol, n-butyl alcohol, ethyl acetate, DMS, acetonitril, benzene, isopropyl alcohol, water, DMF, DCM, DIO, THF, ethanol, and octonol) were investigated at constant dye concentrations. Small changes in the fluorescence spectrum were observed for the different solvents; the highest fluorescence intensity was observed for DMS and the lowest for water. The stokes shift in different solvents was studied for the 5ABBM molecule. This results in the molecule being more polar in the excited state than in the ground state for the used solvents. The ground statedipole moments, HOMO-LUMO, and molecule electrostatic potential map were also computed using ab initio calculations and evaluated using Gaussian 09 W software.
Simultaneous determination of ethyleneamines by reversed-phase ion-pair chromatography with ultraviolet detection using on-line complexation with copper(II) ion
Authors: Yudo Azusa , Kemmei Tomoko , Kodama Shuji , Inoue Yoshinori , Kagaya Shigehiro 
Published: March 2024

Abstract
A selective and simple method was developed for the determination of four ethyleneamines (EAs), i.e., ethylenediamine (EDA), diethylenetriamine (DETA), triethylenetetramine (TETA), and tetraethylenepentamine (TEPA), by reversed-phase ion-pair chromatography with ultraviolet detection. The method is based on the on-line complexation of EAs with a Cu(II) ion added to the acetate buffer mobile phase (pH 5.5). The Cu(II) complexes with EAs (Cu(II)-EA complexes) were well separated on a reversed-phased column, using 1-octanesulfonate as an ion-pairing reagent. The concentrations of 1-octanesulfonate and acetonitrile in the mobile phase significantly influenced the retention times of EAs. The high concentration of the Cu(II) ion in the mobile phase resulted in the increase of the baseline noise. Optimum mobile phase conditions were as follows: 0.5 mM CuSO4, 50 mM acetate buffer (pH 5.5), 20 mM 1-octanesulfonate, and 23% (v/v) acetonitrile. Cu(II)-EA complexes could be detected at 243 nm. The limits of quantification of EAs (S/N = 10) were 0.003 mM for EDA, DETA, and TETA and 0.01 mM for TEPA. The calibration curves were linear over three orders of magnitude of EA concentrations with high correlation coefficients (r2 = 1.000). The proposed method was successfully applied to determine EAs in epoxy resin curing agents.
Evaluation of PVC@Silver nanocomposite as sensor for low limit detection of Cadmium by SPR based method
Authors: Ali Safaa S. , Ebnalwaled Khalid , Mohamed Gehad G. , Hafez M. 
Published: March 2024

Abstract

This study shows the critical effects of Cadmium Ions contamination which is widely spread specially in industrial field, and this has a dangerous impact on both human and the environment. This study is focusing on the development and exploration of a PVC@Silver nanocomposite to enhance the accurate detection of Cadmium ions in environmental and drinking water samples.
Exfoliated graphite for sorption of liquid hydrocarbons from the water surface: Effect of preparation conditions on sorption capacity and water wettability
Authors: Ivanov Andrei V. , Divitskaya Daria A. , Lavrin Maksim A. , Kravtsov Alexei V. , Volkova Svetlana I. , Maksimova Natalia V. , Kalachev Igor L. , Kirichenko Alexey N. , Rodionov Nikolai B. , Malakho Artem P. , Avdeev Victor V. 
Published: March 2024

Abstract
Exfoliated graphite (EG) is a promising macroporous sorbent for oils and liquid hydrocarbons on water surfaces. The preparation of EG includes a synthesis of graphite intercalation compounds (GIC), expandable graphite and its thermal exfoliation. The structure of the initial GIC has a significant influence on the structure of exfoliated graphite and its sorption properties: sorption capacity and selectivity of water/octane sorption. Thus, the aim of this work was to investigate the relationship between the structure of EG based on GIC of 1–4 stages and EG sorption properties and water wettability. The influence of the GIC stage number on the EG sorption and surface properties is studied. EG obtained from 1-stage GIC at 1000°C is characterized by a higher sorption capacity toward octane than EG from 4-stage GIC. The selectivity of octane/water sorption reduces when increasing the GIC stage number from 1 to 4. The ability to sorb water can be explained by a higher surface area of EG and the presence of remaining oxygen groups on the edges of graphite crystallites in the EG structure. The EG structure was investigated by XRD, SEM, nitrogen adsorption-desorption method, FTIR and Raman spectroscopy.
Carrageenan maintains the contractile phenotype of vascular smooth muscle cells by increasing Macromolecular crowding in vitro
Authors: Liu Qing , Jiang Hong-Jing , Wu Yin-Di , Li Jian-Dong , Sun Xu-Heng , Xiao Cong , Xu Jian-Yi , Lin Zhan-Yi 
Published: March 2024

Abstract

Background
The contractile phenotype of vascular smooth muscle cells (VSMCs) has a good diastolic and contractile capacity, and their altered function is the main pathophysiological basis for diseases such as hypertension. VSMCs always exist in a synthetic phenotype in vitro, making it challenging to maintain the contractile phenotype for research. It is widely recognized that common medium in vitro is significantly less crowded than the in vivo environment. Additionally, VSMCs have a heightened sense of touch to detect medium crowding changes. However, it is unclear whether macromolecular crowding (MMC) can help maintain the contractile phenotype of VSMCs.
Purpose
To study and obtain the phenotypic, behavioral and gene expression changes of VSMCs after increasing the crowding degree by adding carrageenan (CR).
Methods
The medium crowding degree was examined by the dynamic light scattering assay;VSMCs survival and activity were examined by the Calcein/PI cell activity and toxicity assay and CCK-8 assays༛VSMCs phenotype and migration were examined by WB and wound healing assay༛Gene expression was examined by transcriptomic analysis and RT-qPCR.
Results
225 µg/mL CR significantly increased the crowding degree of the medium and did not affect cell survival. Simultaneously, CR significantly promoted contraction phenotypic markers expression in VSMCs, cell length was shortened, cell proliferation was decreased, and cell migration was inhibited. CR significantly altered gene expression in VSMCs. Specifically, 856 genes were up-regulated and 1207 genes were down-regulated. These alterations primarily affected cellular ion channel transport, microtubule movement, cellular respiratory metabolism, amino acid transport, and extracellular matrix synthesis. The up-regulated genes were primarily involved in the cytoskeleton and the contraction process of VSMCs, while the down-regulated genes were mainly involved in extracellular matrix synthesis.
Conclusion
The study showed that VSMCs in vitro can maintain the contractile phenotype by sensing changes in the culture environment's crowding, which can be maintained by adding a concentration of CR.
Association between PM2.5 from a coal mine fire and eosinophilic airway inflammation 7.5 years later
Authors: Kress Sara , Lane Tyler J. , Brown David , Smith Catherine L. , Gao Caroline X. , McCrabb Thomas , Thomas Mikayla , Borg Brigitte M. , Thompson Bruce R. , Abramson Michael J. 
Published: March 2024

Abstract
Background and aim:
There are few long-term studies of respiratory health effects of landscape fires, despite increasing frequency and intensity due to climate change. We investigated the association between exposure to coal mine fire PM2.5 and eosinophilic airway inflammation 7.5 years later.
Methods
Adult residents of Morwell, who were exposed to the 2014 Hazelwood mine fire over 6 weeks, and unexposed residents of Sale, participated in the Hazelwood Health Study Respiratory Stream clinical assessments in 2021, including measurements of fractional exhaled nitric oxide (FeNO) concentration, a marker of eosinophilic airway inflammation. Individual exposure to coal mine fire PM2.5 was modelled and mapped to time-location diaries. The effect of exposure to PM2.5 on log-transformed FeNO in exhaled breath was investigated using multivariate linear regression models in the entire sample and stratified by potentially vulnerable subgroups.
Results
A total of 326 adults (mean age: 57 years) had FeNO measured. The median FeNO level (interquartile range [IQR]) was 17.5 [15.0] ppb, and individual exposure to coal mine fire PM2.5 was 7.2 [13.8] µg/m3. We did not identify evidence of association between coal mine fire PM2.5 exposure and FeNO in the general adult sample, nor in various potentially vulnerable subgroups. The point estimates were consistently close to zero in the total sample and subgroups.
Conclusion
Despite previous short-term impacts on FeNO and respiratory health outcomes in the medium term, we found no evidence that PM2.5 from the Hazelwood coal mine fire was associated with any long-term impact on eosinophilic airway inflammation.
Effect of BTCA crosslinking on functional properties of of textiles treated with biomasses from Annona Squamosa and Moringa Oleifera
Authors: Sornapudi Sirisha Deepthi , Srivast Meenu , Manchikatla Srinivas , Thavaraj H. Samuel , kumar B.Senthil 
Published: March 2024

Abstract

Two natural extracts were produced with annona squamosa and moringa Oleifera leaves through the ethanol-based solvent. 100% cotton and 80%:20% polyester/cotton blends with an areal density of 113.5 g/m2 and 101 g/m2, respectively. Eight different samples were produced by coating on natural extract as raw form and BTCA cross-linked form on cotton and polyester fabrics. SEM investigation was also done for all the test fabrics. These coated fabrics were investigated for their antimicrobial activity, wicking properties, stiffness, and crease recovery properties. It was found that the BTCA cross-linked fabrics showed higher antimicrobial activity against gram-positive and gram-negative bacteria. Similarly, the percentage crease recovery angle was higher for the annona squamosa coated sample than moringa Oleifera leave extract in both cotton and P/C samples. Further, it was reported that no significant difference in stiffness values was found between the control samples of cotton and P/C against the treated samples. It was interesting to note that treating the fabrics with cross-linker has improved the vertical wicking property and brought the values closer to control fabric values. This could be interpreted as more hydroxyl groups in the cross linking compound have supported more water penetration.
Association between serum albumin-creatinine ratio and survival in patients treated with immune checkpoint inhibitors
Authors: Baş Onur , Tokatlı Mert , Güdük Naciye , Erdoğan Dilara , Boyraz Nur Evşan , Kavgacı Gözde , Şahin Taha Koray , Güven Deniz Can , Kertmen Neyran , Aksoy Sercan , Erman Mustafa , Yalçın Şuayib , Dizdar Ömer 
Published: March 2024

Abstract
Background
The objective of this study is to assess the correlation between survival outcomes and sACR in patients who are treated with immune checkpoint inhibitors (ICIs).
Methods
This study was conducted on individuals who were administered ICI at least 3 doses from 2018 to 2023. Serum-albumin creatinine ratio (sACR) was measured using serum albumin and creatinine values before the immunotherapy. Based on the median level, the patients were divided into two groups: sACR-High and sACR-Low. The relationship between sACR and progression-free survival (PFS) and overall survival (OS), was estimated using the Kaplan-Meier method along with long-rank testing and Cox proportional hazard models. The relationship between sACR and early progression, late progression, and long-term benefit was estimated using a logistic regression method.
Results
A total of 434 patients were included. Patients were divided into sACR-low and high subgroups according to the median. In the multivariate analyses, patients with lower sACR had decreased OS (HR:1.42, 95% CI 1.07–1.89 p = 0.014) and PFS (HR:1,36, 95% CI 1.09–1.70, p = 0.007). Also, after adjustment for gender, age, and Eastern Cooperative Oncology Group (ECOG) score; sACR was associated with early progression ( HR: 1.86 95% CI, 1.14–3.01 p = 0.012), late progression (HR: 2.06, 95% CI 1.0-4.24 p = 0.050) and long-term benefit of ICIs (HR: 1.72, 95% CI 1.002–2.93 p = 0.049).
Conclusions
It was found that sACR, a marker easily obtained through routine biochemistry testing at low cost, could serve as an independent predictor of PFS and OS patients undergoing immunotherapy. Routine lab tests performed for cancer patients may help clinicians identify high-risk patients in whom closer follow-up or protective measures should be considered.
Causal role of immune cells in major depressive disorder and bipolar disorder: Mendelian randomization (MR) study
Authors: Zhang Yi , Wang San-Wang , Ding Jiahao , Wen Xin , Li Tingting , Yang Lu , Peng Jintao , Dong Yingying , Mi Weifeng , Gao Yujun , Sun GuiZhi 
Published: March 2024

Abstract

Major depressive disorder (MDD) and bipolar disorder (BD) are prevalent psychiatric conditions linked to inflammatory processes. This study employs a two-sample Mendelian randomization (MR) approach to investigate the relationship between immune cells and the risk of MDD and BD. Rigorous sensitivity analyses are conducted to assess the reliability, heterogeneity, and horizontal pleiotropy of the findings. The study reveals a significant association between immune cell profiles and the susceptibility to MDD and BD. Specifically, 29 immunophenotypes exhibit a substantial connection with MDD risk, including CD27 on IgD+ CD38− unswitched memory B cell (inverse variance weighting (IVW): odds ratio(OR) [95%]: 1.017[1.007 to 1.027], p = 0.001), CD27 on IgD+ CD24+ (IVW: OR [95%]: 1.021 [1.011 to 1.031], p = 4.821×10 − 5), CD4 on Central Memory CD4+ T cell(IVW: OR [95%]:0.979 [0.963 to 0.995], p = 0.011) and the other 25 immunophenotypes. Additionally, 35 immunophenotypes demonstrate a significant association with BD risk, such as CD33br HLA DR+ AC AC (IVW: OR [95%]: 1.022[1.007 to 1.036], p = 0.007), CD28+ CD45RA- CD8br %T cell RC (IVW: OR [95%]: 1.024 [1.008 to 1.041], p = 0.004) CD62L on CD62L+ myeloid DC MFI (IVW: OR [95%]:0.926 [0.871 to 0.985], p = 0.014)), and the other 32 immunophenotypes. This MR study provides robust evidence supporting a causal relationship between immune cells and the susceptibility to MDD and BD, offering valuable insights for future clinical investigations.