bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caffeine and carbohydrate supplementation during exercise when in negative energy balance: effects on performance, metabolism, and salivary cortisol
Authors: Slivka Dustin , Hailes Walter , Cuddy John , Ruby Brent 
Published: December 2008

The ingestion of carbohydrate (+CHO) and caffeine (+CAF) during exercise is a commonly used ergogenic practice. Investigations are typically conducted with subjects who are in a rested state after an overnight fast. However, this state of positive energy balance is not achieved during many work and exercise circumstances. The aim of this study was to evaluate the substrate use and performance effects of caffeine and carbohydrate consumed alone and in combination while participants were in negative energy balance. Male participants (n = 9; 23 ± 3 years; 74.1 ± 10.6 kg) completed 4 trials in random order: –CAF/–CHO, –CAF/+CHO, +CAF/–CHO, and +CAF/+CHO. Diet and exercise were prescribed for 2 days before each trial to ensure negative energy balance. For each trial, before and after 2 h of cycling at 50% of maximal watts, a saliva sample and a muscle biopsy (vastus lateralis) were obtained. A simulated 20 km time trial was then performed. The respiratory exchange ratio was higher (p < 0.05) in +CHO trials and lower (p < 0.05) in the +CAF/+CHO trial than in the –CAF/+CHO trial. Salivary cortisol response was higher (p < 0.05) in the +CAF/–CHO trial than in any of the other trials. Muscle glycogen and heart rates were similar in all trials. Performance in the 20 km time trial was better in the –CAF/+CHO trial than in the –CAF/–CHO trial (p < 0.05), but the +CAF/+CHO trial was no better than the +CAF/–CHO trial (p > 0.05), or any of the other trials. When co-ingested with carbohydrate, caffeine increased fat use and decreased nonmuscle glycogen carbohydrate use over carbohydrate alone when participants are in negative energy balance; however, caffeine had no effect on the 20 km cycling time trial performance.
Conjugated linoleic acid prevents growth attenuation induced by corticosteroid administration and increases bone mineral content in young rats
Authors: Roy Brian. D. , Bourgeois Jacqueline , Rodriguez Christine , Payne Eric , Young Kathleen , Shaughnessy Stephen G. , Tarnopolosky Mark A. 
Published: December 2008

Corticosteroids are a common therapy in many disease states, despite frequent and potentially serious side effects. Nutritional supplementation with conjugated linoleic acid (CLA) has been shown to increase fat-free mass, whereas supplementation with n-3 and n-6 fatty acids has been shown to increase bone mineral density (BMD). To determine whether CLA can attenuate the side effects of 8 weeks of corticosteroid administration, we randomized twenty-four 5-week-old male Sprague–Dawley rats into 1 of 4 groups: control; control + methylprednisolone (7 mg·kg–1·week–1); CLA diet (1% CLA w/w); or CLA plus methylprednisolone. Body composition, bone mineral content (BMC), and BMD were assessed with dual-energy X-ray absorptiometry at the onset and at the end of the 8-week intervention. The mechanical properties of bone were determined using 3-point femur bending at the end of the intervention. Methylprednisolone resulted in an attenuation of the increase in body mass and lean mass over the 8 weeks (p < 0.05). CLA prevented the methylprednisolone-induced attenuation of body mass and lean mass accumulation. CLA also resulted in a greater increase in BMC (p < 0.05) in the lumbar spine. The energy at failure of the isolated femurs was increased with CLA (p < 0.05). Dietary CLA prevents many of the growth- and bone-related side effects arising from 8 weeks of corticosteroid administration, results in greater increases in BMC and BMD, and can contribute to an improvement in some of the mechanical properties of bone.
Critical power in adolescent boys and girls — an exploratory study
Authors: Williams Craig A. , Dekerle Jeanne , McGawley Kerry , Berthoin Serge , Carter Helen 
Published: December 2008

The purpose of the study was to identify critical power (CP) in boys and girls and to examine the physiological responses to exercise at and 10% above CP (CP+10%) in a sub-group of boys. Nine boys and 9 girls (mean age 12.3 (0.5) y performed 3 constant-load tests to derive CP. Eight of the boys then exercised, in random order, at CP and CP+10% until volitional exhaustion. CP was 123 (28) and 91 (26) W for boys and girls, respectively (p < 0.02), which was equivalent to 75 (6) and 72 (10) % of peak oxygen uptake, respectively (p > 0.47). Boys’ time to exhaustion at CP was 18 min 37 s (4 min 13 s), which was significantly longer (p < 0.007) than that at CP+10% (9 min 42 s (2 min 31 s)). End-exercise values for blood lactate concentration (B[La]) and maximal oxygen uptake were higher in the CP+10% trial (5.0 (2.4) mmol·L–1 and 2.15 (0.4) L·min–1, respectively) than in the CP trial, (B[La], 4.7 (2.1) mmol·L–1; maximal oxygen uptake, 2.05 (0.35) L·min–1; p > 0.13). Peak oxygen uptake (expressed as a percentage of the peak value) was not attained at the end of the trials (94 (12) and 98 (14) % for CP and CP+10%, respectively). These results provide information about the boundary between the heavy and severe exercise intensity domains in children, and have demonstrated that CP in a group of boys does not represent a sustainable steady-state intensity of exercise.
Time course alterations of plasma obestatin and growth hormone levels in response to short-term anaerobic exercise training in college women
Authors: Manshouri Mahnaz , Ghanbari-Niaki Abbass , Kraemer Robert R. , Shemshaki Afsaneh 
Published: December 2008

Obestatin is a 23-amino-acid peptide encoded by the same gene as ghrelin. Because there is a paucity of data concerning the effects of exercise on obestatin, this study investigated the effect of anaerobic exercise training–detraining on obestatin and growth hormone (GH) plasma levels. Ten young female volunteers participated. Blood was collected 24 h before, and 24 h, 48 h and 7 days after the training. The data indicate that anaerobic training caused a significant reduction in GH levels but had no effect on plasma obestatin concentrations.
Effects of xanthine oxidase inhibition on oxidative stress and swimming performance in rats
Authors: Veskoukis Aristidis S. , Nikolaidis Michalis G. , Kyparos Antonios , Kokkinos Dimitrios , Nepka Charitini , Barbanis Sotiris , Kouretas Dimitrios 
Published: December 2008

The aim of this study was to examine the effect of allopurinol, a xanthine oxidase inhibitor, on oxidative stress and physical performance after swimming until exhaustion in rats. Blood and gastrocnemius muscle samples were collected before, immediately after, and 5 h after exercise and the respective timepoints after allopurinol administration. Xanthine oxidase and total antioxidant capacity (TAC) were determined in plasma and muscle, whereas catalase activity and reduced (GSH) and oxidized (GSSG) glutathione were measured in erythrocytes and muscle. Thiobarbituric acid–reactive substances (TBARS) and protein carbonyls (PC) were determined in plasma, erythrocytes, and muscle. As expected, allopurinol inhibited xanthine oxidase activity. Compared with their nonallopurinol-treated counterparts, rats treated with allopurinol showed a 35% decrease in physical performance, as indicated by the shorter swimming time to exhaustion. Exercise alone increased PC and TBARS concentration in plasma, erythrocytes, and gastrocnemius muscle. Similarly, allopurinol alone increased PC and TBARS concentration in erythrocytes and gastrocnemius muscle, decreased TAC in plasma and gastrocnemius muscle, and decreased the GSH:GSSG ratio in erythrocytes. Our data illustrate that, in general, exercise and allopurinol alone increased the levels of most of the oxidative stress markers measured in plasma, erythrocytes, and gastrocnemius muscle. Xanthine oxidase inhibition provoked a marked reduction in physical performance.
Between-day variability of net and gross oxygen uptake during graded treadmill walking: effects of different walking intensities on the reliability of locomotion economy
Authors: Vilhena de Mendonça Gonçalo , Pereira Fernando Duarte 
Published: December 2008

There have been few studies of clinical relevance conducted on the reliability of walking economy. This study was designed to determine if walking economy reproducibility increases as a function of walking intensity, and if there is any advantage in expressing walking economy as net oxygen uptake (VO2) rather than gross VO2 for reproducibility purposes. Sixteen participants (9 males, 7 females; mean age, 22.3 ± 4.3 years) performed resting, submaximal, and maximal protocols on 2 different days, under identical circumstances, within a 7 day period. The submaximal protocol consisted of five 5 min walks (4 km·h–1) at treadmill grades of 0%, 2.5%, 5.0%, 7.5%, and 10%. Findings indicate that increments of 2.5% in treadmill grade effectively increased gross and net VO2 during walks. The reliability of net and gross measures increased as a function of walking relative intensity, reporting intraclass correlation coefficients ranging from 0.89–0.94 and 0.87–0.91, respectively, and mean coefficients of variation (CV) from 7.3%–3.6% and 8.8%–4.4%, respectively. There were no significant differences between the CV for gross and for net VO2 across the spectrum of walking relative intensities. In conclusion, there is no advantage of expressing walking economy as net VO2 instead of gross VO2 for reproducibility purposes, and a single treadmill testing session at a constant speed of 4 km·h–1 is reliable for estimating group and individual walking economy, particularly at higher percent grades.
Effects of aerobic training on the exercise-induced decline in short-passing ability in junior soccer players
Authors: Impellizzeri Franco M. , Rampinini Ermanno , Maffiuletti Nicola A. , Castagna Carlo , Bizzini Mario , Wisløff Ulrik 
Published: December 2008

To examine the effects of aerobic interval training on the decline in short-passing ability caused by a short bout of high-intensity intermittent activities. For this randomized controlled trial, 26 junior soccer players (mean age, 17.8 ± 0.6 years; mean height, 178 ± 5 cm; mean body mass, 74.5 ± 6.9 kg) were recruited. After baseline measurements, subjects were randomly allocated to 1 of 2 groups: the control group (CG) or the aerobic interval training group (ITG). The ITG completed 4 weeks of high-intensity aerobic training, consisting of 4 bouts of running for 4 min at 90%–95% of maximal heart rate, with 3 min of active recovery between sets, in addition to normal training. Maximum oxygen uptake, Yo-Yo Intermittent Recovery Test level 1 (YYIRT), and short-passing ability (measured using the Loughborough Soccer Passing Test (LSPT)) were measured before and after a 5 min high-intensity simulation (HIS), reproducing the most intense phase of a match. The ITG (n = 11), but not the CG (n = 10), showed a significant 12% and 4% increase in YYIRT and maximal oxygen consumption after training, respectively, and reduced the worsening in LSPT penalty time after the HIS (p < 0.05). The relative exercise intensity during HIS decreased in the ITG only (p < 0.01). Our results demonstrated that junior soccer players may benefit from aerobic training to attenuate the decline in short-passing ability caused by a short bout of intermittent activities completed at the same pretraining workload.
Coffee consumption and risk of type 2 diabetes, cardiovascular diseases, and cancer
Authors: van Dam Rob M. 
Published: December 2008

Numerous epidemiological studies have evaluated the association between coffee consumption and risk of type 2 diabetes, coronary heart disease, and various cancers. This paper briefly reviews the evidence for a relation between coffee consumption and these conditions, with particular attention to methodological issues. Several early studies suggested that coffee consumption could result in a marked increase in risk of coronary heart disease and several types of cancer. However, more recent prospective cohort studies that are less prone to selection and information bias have not confirmed these findings. High consumption of unfiltered types of coffee, such as French press and boiled coffee, has been shown to increase low-density-lipoprotein-cholesterol concentrations. In addition, limiting caffeinated coffee intake during pregnancy seems a prudent choice. However, evidence has been accumulating that frequent consumption of coffee may reduce risk of type 2 diabetes and liver cancer. Further experimental studies are warranted to elucidate the underlying mechanisms and possibly identify the components in coffee that are responsible for these putative effects. In sum, the currently available evidence on coffee and risk of cardiovascular diseases and cancer is largely reassuring, and suggests that, for the general population, addressing other health-related behaviors has priority for the prevention of chronic diseases.
Precision and reliability of strength (Jamar vs. Biodex handgrip) and body composition (dual-energy X-ray absorptiometry vs. bioimpedance analysis) measurements in advanced cancer patients
Authors: Trutschnigg Barbara , Kilgour Robert D. , Reinglas Jason , Rosenthall Leonard , Hornby Laura , Morais José A. , Vigano Antonio 
Published: December 2008

Important deteriorations in body composition and strength occur and need to be accurately measured in advanced cancer patients (ACPs). The aim of this study was to establish the relationship between a single-frequency bioimpedance analyzer (BIA) and the dual-energy X-ray absorptiometer (DXA), as well as the Jamar handgrip dynometer and the Biodex handgrip attachment, and to determine the precision of each of these instruments in ACPs. Eighty-one ACPs with non-small-cell lung cancer and gastrointestinal cancer were recruited from the McGill University Health Centre (Montreal, Que.). Consecutive paired measurements, with repositioning between measurements, were obtained for total-body DXA, BIA, Biodex handgrip, and BIA plus Jamar handgrip. The total-body percent coefficient of variation (%CV) for the BIA and DXA were 1.34 and 1.56 for fat mass (FM), respectively, and 0.42 and 0.72 for fat free mass (FFM), respectively. The %CV for the Jamar and Biodex handgrips were 6.3 and 16.7, respectively. Bland–Altman plots were used to characterize the limits of agreement between DXA and BIA for FM (4.60 ± 7.80 (–3.19 to 12.39) kg) and FFM (–1.87 ± 7.16 (–9.03 to 5.29) kg). Both DXA and BIA demonstrate good short-term precision in ACPs. However, given its poor accuracy, it remains to be determined if BIA can be used to monitor ACPs for changes in total-body tissue composition as a function of time, whether for observation or response to treatment. Furthermore, because of wide limits of agreement, the DXA and BIA cannot be used interchangeably in research or clinical settings. The Jamar handgrip dynamometer shows more consistency than the Biodex handgrip attachment in ACPs, and should therefore be the preferred measure of changes in strength over time.
Structural alterations of erythrocyte membrane components induced by exhaustive exercise
Authors: Brzeszczynska Joanna , Pieniazek Anna , Gwozdzinski Lukasz , Gwozdzinski Krzysztof , Jegier Anna 
Published: December 2008

Physical exercise was used as a model of the physiological modulator of free radical production to examine the effects of exercise-induced oxidative modifications on the physico-biochemical properties of erythrocyte membrane. The aim of our work was to investigate conformational changes of erythrocyte membrane proteins, membrane fluidity, and membrane susceptibility to disintegration. Venous blood was taken before, immediately after, and 1 h after an exhaustive incremental cycling test (30 W·min–1ramp), performed by 11 healthy untrained males on balanced diets (mean age, 22 ± 2 years; mean body mass index, 25 ± 4.5 kg·m–2). In response to this exercise, individual maximum heart rate was 195 ± 12 beats·min–1and maximum wattage was 292 ± 27 W. Electron paramagnetic resonance spectroscopy was used to investigate alterations in membrane proteins and membrane dynamics, and to measure production of radical species. The reducing potential of plasma (RPP) was measured using the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric-reducing ability of plasma. Exercise induced decreases in erythrocyte membrane fluidity in the polar region (p < 0.0001) and alterations in the conformational state of membrane proteins (p < 0.05). An increase in RPP was observed immediately after exercise (p < 0.001), with a further increase 1 h postexercise (p < 0.0001). Supporting measurements of lipid peroxidation showed an increase in thiobarbituric acid reactive substances immediately after exercise (p < 0.05) and at 1 h of recovery (p < 0.001); however, free radicals were not detected. Results indicate the existence of early postexercise mild oxidative stress after single-exercise performance, which induced structural changes in erythrocyte membrane components (protein aggregation) and in the membrane organization (lipids rigidization) that followed lipid peroxidation but did not lead to cellular hemolysis.
Strength predictions of pile caps by a strut-and-tie model approach
Authors: Park JungWoong , Kuchma Daniel , Souza Rafael 
Published: December 2008

In this paper, a strut-and-tie model approach is presented for calculating the strength of reinforced concrete pile caps. The proposed method employs constitutive laws for cracked reinforced concrete and considers strain compatibility. This method is used to calculate the load-carrying capacity of 116 pile caps that have been tested to failure in structural research laboratories. This method is illustrated to provide more accurate estimates of behavior and capacity than the special provisions for slabs and footings of the 1999 American Concrete Institute (ACI) code, the pile cap provisions in the 2002 CRSI design handbook, and the strut-and-tie model provisions in either the 2005 ACI code or the 2004 Canadian Standards Association (CSA) A23.3 standard. The comparison shows that the proposed method consistently well predicts the strengths of pile caps with shear span-to-depth ratios ranging from 0.49 to 1.8 and concrete strengths less than 41 MPa. The proposed approach provides valuable insight into the design and behavior of pile caps.
Design of stilling basin for circular pipe outlets
Authors: Goel Arun 
Published: December 2008

Experimental work leading to the design of a stilling basin for pipe outlets is reported in this paper. The stilling basin designed on the basis of present study has been compared with the stilling basin design proposed by Garde et al. in 1986. The experiments were performed by taking two pipe outlets of 10 and 7.5 cm diameter and varying inflow Froude numbers. In the present study, the new design is arrived at by conducting a systematic experimentation on the various stilling basin models and keeping the basin floor at the invert level of the pipe outlet. The location as well as the arrangements of appurtenances, such as a wedge-shaped splitter block, modified grid, row of wedge-shaped baffle blocks, and end sill, were also varied in the experiments. A nondimensional number has been defined to compare the performance of the stilling basin models on the basis of developed scour downstream of the basin. The performance of the proposed stilling basin (model M-71) is much better as compared with that of the stilling basin G-D* proposed by Garde et al. in 1986.
Analytical estimation of effective discharge for small southern Ontario streams
Authors: Quader A. , Guo Y. , Stedinger J. R. 
Published: December 2008

The effective discharge (Qe) of a channel reach may be analytically determined by fitting the flow–duration relationship with a distribution model and representing the bed-material transport with a power function. Analysis of streamflow data from small southern Ontario streams showed that the commonly used lognormal distribution does not provide a good fit to many streamflow records. A mixed exponential distribution model is proposed and a formula for computing the resulting Qe is derived. The results from this new Qe formula were compared with those obtained using other analytical distribution functions and those from empirical sediment transport effectiveness curves. These comparisons indicate that the new analytical solution provides on average the most accurate estimates of Qe when the exponent β of the power function describing sediment transport at the channel reach is not more than 2; when β > 2, a gamma distribution provides the most accurate estimates followed closely by the mixed exponential distribution.
Encapsulation of glutamine synthetase in mouse erythrocytes: a new procedure for ammonia detoxification
Authors: Kosenko Elena A. , Venediktova Natalia I. , Kudryavtsev Andrey A. , Ataullakhanov Fazoil I. , Kaminsky Yury G. , Felipo Vicente , Montoliu Carmina 
Published: December 2008

There are a number of pathological situations in which ammonia levels increase leading to hyperammonemia, which may cause neurological alterations and can lead to coma and death. Currently, there are no efficient treatments allowing rapid and sustained decrease of ammonia levels in these situations. A way to increase ammonia detoxification would be to increase its incorporation in glutamine by glutamine synthetase. The aim of this work was to develop a procedure to encapsulate glutamine synthetase in mouse erythrocytes and to assess whether administration of these erythrocytes containing glutamine synthetase (GS) reduce ammonia levels in hyperammonemic mice. The procedure developed allowed the encapsulation of 3 ± 0.25 IU of GS / mL of erythrocytes with a 70% cell recovery. Most metabolites, including ATP, remained unaltered in glutamine synthetase-loaded erythrocytes (named ammocytes by us) compared with native erythrocytes. The glutamine synthetase-loaded ammocytes injected in mice survived and retained essentially all of their glutamine synthetase activity for at least 48 h in vivo. Injection of these ammocytes into hyperammonemic mice reduced ammonia levels in the blood by about 50%. The results reported indicate that ammocytes are able to keep their integrity, normal energy metabolism, the inserted glutamine synthetase activity, and can be useful to reduce ammonia levels in hyperammonemic situations.