bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence of the Enigmatic, Unicellular Psorospermium Organism in Several Cultured, Sympatric Populations of the Freshwater Crayfishes Procambarus clarkii and Procambarus zonangulus
Authors: Klarberg David P. , Henttonen Paula , Huner Jay V. 
Published: April 2007

AbstractThe parasitic Psorospermium organism was studied in cultured sympatric populations of mature Procambarus clarkii and Procambarus zonangulus during four consecutive annual crayfish seasons from 1991–1992 through 1994–1995. Most P. clarkii were infected while fewer than half of the P. zonangulus were infected throughout the study period. The numbers of Psorospermium were four to eight times greater in P. clarkii and showed annual changes not seen in P. zonangulus. Both crayfishes demonstrate 1yr life cycles in Louisiana and this seemed to explain the variations seen in Psorospermium dynamics in the two hosts. In general, there was no consistent association between crayfish size and the number of parasites found in them for either crayfish species. There was some indication of an association between the presence of infection and the number of parasites found in P. zonangulus.
Effect of Environmental Factors on Development of Rotifer and Copepod Nauplii Populations in Sunshine Bass Morone chrysops×M. saxatilis Nursery Ponds without Fish
Authors: Ludwig Gerald M. 
Published: April 2007

AbstractKnowledge of the effect of environmental factors on timing and production of zooplankton populations would enable sunshine bass Morone chrysops×M. saxatilis farmers to stock fry when live food is most available and thereby increase fry survival. Relationships of morning water temperature, morning and average daily air temperature, day length, number of rain days and daily rain fall, and dissolved oxygen concentrations to time required for rotifer and copepod nauplii populations to peak in freshly filled freshwater nursery ponds was determined. Relationships of the same independent variables to concentrations of rotifers and copepod nauplii at the peak were also determined. For rotifers, day length, dissolved oxygen, and water and air temperatures were highly correlated with time to the peak density, but not concentration of rotifers. Time required for copepod nauplii densities to peak was associated with rain events; whereas, nauplii concentration was related to day length, dissolved oxygen levels and, to a lesser extent, water and air temperatures. Regression equations of the independent variables had very low predictive value for rotifer or nauplii densities or time to reach initial peak nauplii populations. Regressions of day length, morning water temperature, morning and average daily air temperature had moderate values (r2 > 0.7) for predicting time to initial peak rotifer densities.
Economic Feasibility of Aquaculture of Spiny Lobsters Jasus edwardsii in Temperate Waters
Authors: Jeffs Andrew , Hooker Simon 
Published: April 2007

AbstractAround the world there is strong interest in the aquaculture of spiny lobsters (family Palinuridae). However, there is little published information about the economic feasibility of spiny lobster aquaculture. For more than 20 yr there has been experimental grow out of the spiny lobster Jasus edwardsii in New Zealand in land‐based systems using seed animals (puerulus) taken from the wild. These studies provide sufficient information on growth, mortality, food conversion, handling and capital costs to enable an assessment of the economic feasibility of the commercial culture of spiny lobsters in temperate waters. This assessment suggests that profitable spiny lobster aquaculture will rely on greatly reducing the infrastructure and operating costs of land‐based farming operations, as well as lowering feed and labor costs. Financial simulations suggest that increasing productivity through faster growth rates and lowered culture mortalities will only have a minor effect on profitability unless infrastructure and operating costs can be reduced significantly. Seacage culture or sea ranching of spiny lobsters may offer a means of avoiding high infrastructure costs associated with land‐based farming operations. The development of a cost‐effective artificial feed would also appear to be a priority for improving the economic outlook for culturing spiny lobsters. The results of this study are relevant to the economics of spiny lobster culture developments in other temperate regions of the world.
Concentration of 17α‐Methyltestosterone in HormoneTreated Feed: Effects of Analytical Technique, Fabrication, and Storage Temperature
Authors: Teichert‐Coddington David , Manning Bruce , Eya Jonathan , Brock David 
Published: April 2007

AbstractFeed treated with 17α‐methyltestosterone (MT) is used to manipulate the gender of early tilapia fry. In the USA, hormone‐treated feed is used by selected producers under an Investigational New Animal Drug (INAD) program. While monitoring the fabrication and on‐farm use of this treated feed, concerns were raised about the high performance liquid chromatography (HPLC) analytical procedure for detecting MT in feeds, the incorporation of MT in feeds during fabrication, and effects of storage temperature on MT concentration. A series of experiments demonstrated that the analytical procedure for detecting MT in feeds lacked high precision, and, on average, biased results low. MT was uniformly mixed with feed by spraying an MT‐alcohol solution on feed while it was blended in an industrial ribbon mixer. Alcohol volumes ranging from 15 mL/kg to 150 mL/kg were equally effective at dispersing MT in feed. The concentrations of MT in feeds consistently declined over time if the storage temperatures were 25 C or higher. Freezing preserved the MT in feed, and the refrigeration of feed fabricated to contain 60 mg MT/kg maintained acceptable MT concentrations during 6 mo of storage.
Dry‐Tilling of Pond Bottoms and Calcium Sulfate Treatment for Water Quality Improvement
Authors: Seo Jinwon , Boyd Claude E. 
Published: April 2007

AbstractThree different pond bottom treatments were evaluated in 12 earthen ponds. Bottoms of four ponds on the Auburn University Fisheries Research Unit, Auburn, Alabama, were dried for 1 mo and tilled with a roto‐tiller (dry‐till treatment). Four other ponds were dried and tilled, and after filling with water, enough gypsum (calcium sulfate) was applied to give a total hardness of about 200 mg/L. Gypsum was reapplied as needed to maintain the hardness (dry‐till with gypsum treatment). Four ponds were not subjected to bottom drying, tilling or gypsum treatment (controls). Channel catfish Ictalurus punctatus fingerings were stocked at 15,000/ha. Selected water quality variables were measured at 1‐ to 2‐wk intervals during the growing season. Concentrations of most variables increased over time because feeding rate was increased progressively as fish grew. Compared to the controls, both treatments had lower concentrations of total phosphorus and soluble reactive phosphorus, and higher concentrations of dissolved oxygen (P < 0.05). In addition, ponds of the dry‐till with gypsum treatment had in addition lower concentrations (P < 0.05) of chlorophyll a, chemical oxygen demand, gross primary productivity, and total alkalinity than control ponds. The reduction in chlorophyll a concentration suggested less phytoplankton in gypsum‐treated ponds, and this effect was probably related to lower phosphorus availability because of calcium phosphate formation. Secchi disk visibility, total suspended solids concentrations, and turbidity did not differ significantly among the treatments (P < 0.05). Total fish production and survival rate did not differ significantly (P < 0.05) among the treatments. These findings suggest that water quality improvement can be achieved by drying and tilling pond bottoms between crops, and benefits possibly may be increased by treating low hardness waters with gypsum.
Growth and Survival of the Blood Ark Anadara ovalis (Bruguière, 1789) Cultured in Mesh Bags on Soft‐Bottom Sediments in the Coastal Waters of Georgia
Authors: Power Alan J. , Walker Randal L. 
Published: April 2007

AbstractAnnual growth and survival rates were measured for blood arks Anadara ovalis (Bruguière 1789) cultured in mesh bags that were placed at the spring‐low‐water mark in soft‐bottom sediments of the Skidaway River, Georgia. The feasibility of growing the arks to a marketable size using this technique was assessed by determining the effects of stocking density and biofouling on growth and survival. Two replicate densities of 190 (low) and 400 (high) arks per mesh bag (mean shell length 31.97 mm) were planted in early September 1999. In April 2000, the arks cultured at the lower density had a significantly larger size (44.99 mm) than at the higher density (43.83 mm), with growth rates of 1.85 mdmo, and 1.69 mm/mo, respectively. Growth decreased considerably in the subsequent months (low: 0.17 mm/mo; high: 0.30 mm/mo). There was no significant difference in ark size between treatments after a year's growth in late August 2000 (low: 45.76 mm, 1.15 mm/mo; high: 45.31 mm, 1.11 mm/mo). Similarly, no significant difference in annual survival rates between stocking densities occurred (low: 42.89%; high: 40.25%). The present findings indicate that this method of growing arks to market size has potential to contribute to future endeavors to develop an aquaculture fishery for the blood ark in the coastal waters of Georgia.
The Effect of Temperature on First Feeding, Growth, and Survival of Larval Witch Flounder Glyptocephalus cynoglossus
Authors: Bidwell Deborah A. , Howell W. Huntting 
Published: April 2007

AbstractWitch flounder Glyptocephalus cynoglossus has recently been identified as a candidate species for aquaculture in the northeastern United States and the Canadian Atlantic Provinces. This study investigated the optimal temperatures for witch flounder larval first feeding and for long term larval culture from hatching through metamorphosis. Maximum first feeding occurred between 15.0 and 16.2 C. Larvae did not survive beyond first feeding when reared at mean temperatures of 5.1, 10.4, or 19.5 C and were unable to initiate feeding at mean rearing temperatures below 6.0 C. At a rearing temperature of 15.0 C in 16‐L tanks, mean larval survival to 60 days post hatch (dph) was 14.1%. Mean overall length‐specific growth rate for larvae reared to 60 dph at 15.0 C was 3.5%/d and mean absolute growth was 0.62 mm/d. Subsequent larval growth at 15.6 C began to taper off towards 70 dph at the onset of weaning which overlapped with larval metamorphosis. Growth plateaued at 85 dph, followed by a rebound between 90 and 95 dph. Survival was 100% when weaning onto a dry, pelleted diet was initiated at 70 dph with a 10‐d live diet co‐feeding period. These results are favorable and encourage the further pursuit of commercial witch flounder culture.
Assessment of Five Shelter Types in the Production of Redclaw Crayfish Cherax quadricarinatus (Decapoda: Parastacidae) Under Earthen Pond Conditions
Authors: Jones Clive M. , Ruscoe Ian M. 
Published: April 2007

AbstractAn experiment was designed to assess the relative performance of several shelter types on the production of redclaw crayfish Cherar quadricarinatus under earthen pond conditions. Juvenile crayfish with a mean stocking weight of 12.76 g were cultured in 24 net pens at 12.5 m2 within a 0.2 ha earthen pond, and were provided with one of five shelter types or no shelter over 162 d. The various shelter types assessed were: mesh bundles, pipe stacks, car tires, elevated cement/fiber‐board sheets, and cement/fiber‐board sheets laid flat on the substrate. Crayfish in each pen were fed a formulated pellet diet three times each week. Treatments were assessed on the basis of crayfish growth, survival, harvest biomass and berry rate among females at harvest. There was no significant effect (P > 0.05) of shelter type on growth; however, there was a highly significant effect (P < 0.001) on survival with mesh bundles (75.1%), tires (51.4%), and pipe stacks (43.25%) performing significantly better than the no shelter control (15%). The consequence of equal growth and differing survival among treatments produced significant differences in harvest biomass (P < 0.01). There were also significant differences in berry rate for the different treatments (P= 0.002), and a significant negative correlation of berry rate on survival for those treatments where shelter was provided. This experiment showed that the shelter types provided during pond culture had a significant effect on harvested biomass, primarily through its effect on survival. The efficacy of the mesh bundle shelters may be attributable to the variability in the size of the spaces provided, and the ability of the mesh to separate many individuals relative to the overall volume of the shelter.
Effects of Dietary Fiber on Growth and Gastric Emptying Time of the Freshwater Prawn Macrobrachiurn rosenbergii (De Man, 1879)
Authors: del Carmen González‐Peña Maria , Gomes Santo Z. , Moreira Gloria S. 
Published: April 2007

AbstractFeeding experiments were conducted on large and small adult freshwater prawns Macrobrachium rosenbergii (36.96 ± 1.65 and 13.48 ± 1.65 g in weight) to determine the impact of dietary fiber on growth, digestion, and gastric emptying time. Fiber was incorporated as Q‐cellulose into four semi‐purified diets at calculated levels of 0, 5, 10 and 15% at the expense of starch and fed for an 8‐wk period. The experiment was conducted in a closed, filtered, recirculating freshwater system. The results indicate that the specific growth rates, feed conversion ratios, and protein efficiency ratios improved as the levels of dietary fiber increased from 0.4% to 8%. Gastric emptying times increased as the level of inclusion of cellulose in the diets increased. It was concluded that diets containing up to 10% dietary fiber increases growth rates in adult prawn Macrobrachium rosenbergii by increasing nutrient residence time in the gastrointestinal tract, thereby increasing absorption.
Massive Hepatic Necrosis and Nodular Regeneration in Largemouth Bass Fed Diets High in Available Carbohydrate
Authors: Goodwin Andrew E. , Lochmann Rebecca T. , Tieman Darlene M. , Mitchell Andrew J. 
Published: April 2007

AbstractLargemouth bass Micropterus salmoides are piscivorous fish raised on farms then sold live in Asian fish markets on the east and west coasts of the United States. In the winter of 1998, a major producer of feed‐trained bass suffered a significant increase in fish mortality both during shipping and while the fish were still in ponds. No bacterial, viral, or significant parasitic pathogens were found at necropsy. Livers of affected fish were pale and translucent with 3–10 mm pink nodules on their surface and deeper in the parenchyma. Histological examination of these livers showed that the translucent regions of the liver contained few hepatocytes and were composed of tissue consistent with a chronic inflammatory response. Also present were eosinophils, islands of pancreatic and biliary cells, and granulomas that did not stain positively for mycobacteria. The pink nodules were areas of multifocal regeneration of normal hepatocytes. Sequential studies of bass in ponds revealed that the bass were progressively accumulating glycogen in their hepatocytes to an extent sufficient to explain the massive necrosis of that organ. In order to determine the effect of diets varying in available carbohydrate on fish growth, survival, and liver glycogen content, a 12‐wk feeding trial was conducted in aquaria with juvenile largemouth bass. Nitrogen‐free extract values indicated that an extruded trout diet, a steelhead trout diet, and a diet designed to contain 45% protein and 25% fat, contained 35, 27, and 21% carbohydrate, respectively. Weight gain was lowest in fish fed the extruded trout diet, while liver glycogen was significantly higher in fish fed diets with >27% carbohydrate than in fish fed the diet with 21% carbohydrate. The farmer switched to a diet similar to the 45–25 diet used in our trial. Subsequently, 16‐mo‐old fish examined in October 2000 had no hepatic nodules or necrosis, were significantly larger and less variable in size than previous crops, and the farmer experienced no significant losses in ponds or during shipping.
Evaluation of Experimental and Practical Diets for Walleye Stizostedion vitreum
Authors: Bharadwaj Anant S. , Gould Nathan L. , Brown Paul B. , White Melvin R. , Moore Alan 
Published: April 2007

AbstractCulture of walleye Sfizostedion vitreum is one of the largest components of public sector aquaculture in the eastern U.S. and there is increasing interest in private sector culture. However, the nutritional requirements of walleye are unknown and experimental diets for use in quantifying nutritional requirements have not been identified. We formulated four experimental and four practical diets and fed those to triplicate groups of walleye with an initial weight of 13 g per fish. The experimental diets contained either casein (CAS), casein + gelatin (CG), casein + arginine (CA), or casein + gelatin + crystalline amino acids (CGAA) as sources of amino acids. The practical diets were formulated to mimic salmon grower (SG) and trout grower (TG) diets, a fish meal‐free diet for trout (TFMF), and a walleye grower (WG) diet. Fish were fed twice daily to satiation for 9 wk. Feed consumption, percent weight gain, specific growth rates, feed efficiency, protein efficiency ratio, and protein retention efficiency were not significantly different among fish fed CGAA, SG, and TG, but those values were significantly higher than in fish fed other diets. Weight gain of fish fed CGAA was approximately 80% of that in fish fed SG and 91% of that in fish fed TG. Protein retention efficiency of fish fed CGAA was approximately 69% and 81% of that observed for fish fed SG, and TG, respectively. In general, the carcasses of fish fed diets CGAA, SG and TG had significantly lower moisture and ash concentrations, and higher lipid levels than fish fed other diets. There were no significant differences in carcass protein concentration, muscle proximate composition, or liver lipid concentration among treatments. Livers from fish fed all diets were characterized by microvesicular degeneration and glycogen accumulation in hepatocytes. Results from the study indicate that CGAA can be used as a basal experimental diet in future nutritional research with juvenile walleye and confirms the benefits of trout and salmon grower diets. Fish meal‐free diets formulated around the requirements for rainbow trout were consumed at approximately 80% of the values in fish fed TG and SG, but weight gain was approximately 20% of that in fish fed TG and SG. It appears the nutritional requirements for walleye are different than those of rainbow trout.
Optimizing Prepared Feed Ration for Gonad Production of the Green Sea Urchin Strongylocentrotus droebachiensis
Authors: Pearce Christopher M. , Daggett Tara L. , Robinson Shawn M. C. 
Published: April 2007

AbstractAdult green sea urchins Strongylocentrotus droebachiensis were fed three different quantities (0.25, 0.50, 1.00% body weight/d) of a prepared feed during a 12‐wk experiment to determine the effect of food ration on gonad quantity and quality. A diet of kelp, Laminaria longicruris and/or L. digitata, fed at satiation (3% body weight/d) and urchins taken from the wild at the beginning and end of the experiment served as controls. Urchins fed prepared feed or kelp increased their percent gonad yield significantly over the experimental period. Affer 12 wk, individuals fed the prepared diet at 0.50 or 1.00% body weighffd had significantly higher percent gonad yields (mean ± SEM: 23.5 ± 0.6% and 23.4 ± 0.7%, respectively) than urchins fed at 0.25% body weight/d (18.0 ± 1.0%) or control animals given kelp (18.3 ± 0.8%). There was no significant difference in gonad yield between the 0.50 and 1.00% feeding levels. All feeding treatments had significantly higher percent gonad yields than urchins sampled from the wild at the end of the experiment that had recently spawned (2.8 ± 0.5%). There was no significant difference in gonad taste among urchins fed the prepared diet at 0.25% body weight/d, those given kelp, or those collected from the wild at the end of the experiment (good to very good ratings), but all of these treatments had significantly better tasting gonads than urchins given the prepared feed at 0.50 or 1.00% body weight/d (satisfactory ratings). Gonad taste rating of urchins fed a prepared diet was dependent on ration with greater feed amounts leading to worse tasting gonads (linear regression: r2= 0.68, P < 0.01). Gonad color, texture, and firmness did not differ significantly among any of the feed ration treatments or kelp control. Results suggest that the best ration for prepared feed would be 0.50% body weightld since this would optimize gonad yield while minimizing feed costs. Further research on prepared feeds is required to optimize both gonad color and taste.
Complete Replacement of Fish Meal by Soybean Meal with Dietary L‐Lysine Supplementation for Nile Tilapia Oreochromis niloticus (L.) Fingerlings
Authors: El‐Saidy Deyab M. S. D. , Gaber Magdy M. A. 
Published: April 2007

AbstractThe effect of complete replacement of fish meal by soybean meal (SBM) with the dietary L‐lysine supplementation in prepared diet was studied in Nile tilapia fingerlings. Five isonitrogenous (33.2% crude protein) and isocaloric (4.8 kcal gross energy/g of diet) diets were formulated. Diet 1 was similar to a high quality commercial tilapia diet, containing 20% fish meal and 30% SBM. Diets 2–5 contained 55%, 54%, 53%, and 52% SBM and 0.5%, 1.0%, 1.5%, and 2.0% L‐lysine supplementation, respectively. After 10 wk, there was a significant difference in the final individual weight, final body length (cm), weight gain (%), specific growth rate (SGR), feed conversion ratio (FCR), protein effkiency ratio (PER), and food intake among flsh groups (P≤ 0.05). The best bal individual weight, final individual length (cm), weight gain (%), specific growth rate, feed conversion ratio, protein efficiency ratio (PER), and food intake were recorded in fish fed diet 2, which contained 55% SBM and 0.5% L‐lysine. There was a significant difference between it and diet 1 (control). Moisture in fish flesh was not significantly different (P > 0.05) among treatments and averaged 75.13%, while percentage protein was significantly dltrerent (P≤ 0.0% and the best result was achieved in flsh fed diet 2 and diet 1 (control). The highest values of digestibility coefficients of protein, fat and energy were recorded in groups of fish fed diet 2. These suggest that a diet with 55% SBM supplemented with 0.5% L‐lysine can totally replace fish meal in a diet for Nile tilapia fingerlings, without adverse effect on fish performance.