bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cone beam computed tomography assessment of maxillary anterior teeth cervix dimensions in healthy adults for optimal anatomic healing abutments
Authors: Lu Jie , Ou Yanjing , Zhao Wei , Chen Huachen , He Kaixun , Lin Hanyu , Chen Jiang 
Published: April 2024

AbstractObjectivesThe abutments produced with circular symmetry failed to accurately replicate the natural teeth's cervical shapes. The purpose of this study was to measure cervical cross‐sections of maxillary anterior teeth using cone beam computed tomography (CBCT) images to design anatomic healing abutments.Materials and MethodsCBCT data of 61 patients were analyzed using Ez3D Plus software. Measurements were taken at the cemento‐enamel junction (CEJ) and 1 mm coronal to CEJ for maxillary central incisors, lateral incisors, and canines. Various parameters, including area, perimeter, and eight line segments in the distal (a), disto‐palatal (b), palatal (c), mesio‐palatal (d), mesial (e), mesio‐labial (f), labial (g), and disto‐labial (h) directions, were used to describe dental neck contours. The ratios (f/b and h/d) were analyzed, and differences based on sex and dental arch morphology were explored.ResultsSignificant differences were found in area and perimeter between males and females, but not in f/b and h/d ratios. Differences in the f/b ratio were observed among dental arch morphologies for maxillary central incisors, lateral incisors, and canines.ConclusionsCBCT measurements of cervical cross‐sections provide more accurate data for designing anatomic healing abutments. The fabrication of anatomical healing abutments needs to consider the influence of gender on cervical size and to explore the potential effect of arch shape on cervical morphology.Clinical SignificanceThe novel method provides detailed measurements for the description of dental cervical contours for patients with bilateral homonymous teeth missing. The measurements of this study could be utilized to design more accurate anatomic healing abutments to create desired morphology of peri‐implant soft tissue.
Autophagy receptor ZmNBR1 promotes the autophagic degradation of ZmBRI1a and enhances drought tolerance in maize
Authors: Xiang Yang , Li Guangdong , Li Qian , Niu Yingxue , Pan Yitian , Cheng Yuan , Bian Xiangli , Zhao Chongyang , Wang Yuanhong , Zhang Aying 
Published: April 2024

ABSTRACTDrought stress is a crucial environmental factor that limits plant growth, development, and productivity. Autophagy of misfolded proteins can help alleviate the damage caused in plants experiencing drought. However, the mechanism of autophagy‐mediated drought tolerance in plants remains largely unknown. Here, we cloned the gene for a maize (Zea mays) selective autophagy receptor, NEXT TO BRCA1 GENE 1 (ZmNBR1), and identified its role in the response to drought stress. We observed that drought stress increased the accumulation of autophagosomes. RNA sequencing and reverse transcription‐quantitative polymerase chain reaction showed that ZmNBR1 is markedly induced by drought stress. ZmNBR1 overexpression enhanced drought tolerance, while its knockdown reduced drought tolerance in maize. Our results established that ZmNBR1 mediates the increase in autophagosomes and autophagic activity under drought stress. ZmNBR1 also affects the expression of genes related to autophagy under drought stress. Moreover, we determined that BRASSINOSTEROID INSENSITIVE 1A (ZmBRI1a), a brassinosteroid receptor of the BRI1‐like family, interacts with ZmNBR1. Phenotype analysis showed that ZmBRI1a negatively regulates drought tolerance in maize, and genetic analysis indicated that ZmNBR1 acts upstream of ZmBRI1a in regulating drought tolerance. Furthermore, ZmNBR1 facilitates the autophagic degradation of ZmBRI1a under drought stress. Taken together, our results reveal that ZmNBR1 regulates the expression of autophagy‐related genes, thereby increasing autophagic activity and promoting the autophagic degradation of ZmBRI1a under drought stress, thus enhancing drought tolerance in maize. These findings provide new insights into the autophagy degradation of brassinosteroid signaling components by the autophagy receptor NBR1 under drought stress.
Bilateral inferior petrosal sinus sampling in the differential diagnosis of ACTH‐dependent Cushing's syndrome: A reappraisal
Authors: Valizadeh Majid , Abiri Behnaz , Hosseinpanah Farhad , Grossman Ashley 
Published: April 2024

AbstractCushing's syndrome (CS) is a rare disorder, once exogenous causes have been excluded. However, when diagnosed, the majority of cases are adrenocorticotropic hormone (ACTH)‐dependent, of which a substantial minority are due to a source outside of the pituitary, ectopic ACTH syndrome (EAS). Differentiating among pituitary‐dependent CS, Cushing's disease (CD) and an ectopic source can be problematic. Because non‐invasive tests in the evaluation of CS patients often lack adequate sensitivity and specificity, bilateral inferior petrosal sinus sampling (BIPSS), a minimally invasive procedure performed during the investigation of ACTH‐dependent CS, can be extremely helpful. BIPSS is considered to be the gold standard for differentiating CD from the EAS. Furthermore, although such differentiation may indeed be challenging, BIPSS is itself a complex investigation, especially in recent times due to the widespread withdrawal of corticotrophin‐releasing hormone and its replacement by desmopressin. We review current published data on this investigation and, in the light of this and our own experience, discuss its appropriate use in diagnostic algorithms.
Correlation between centre size, metabolic variation and mean HbA1c in major paediatric diabetes centres
Authors: Swaney Ella EK , McCombe Julia , Donath Susan , Cameron Fergus J ,   Australasian Diabetes Data Network Study Group
Published: April 2024

AimTo exploit a relatively homogeneous national health care context and a national diabetes database to address the questions: Is there an optimal clinic/centre size in determining outcomes?; and Can improvement in median centre outcomes be driven by reducing variability in outcome?MethodsUsing the Australasian Diabetes Database Network, data from seven tertiary hospital paediatric diabetes clinics for patients with type one diabetes from Australia were recorded from 6‐month uploads: September 2017, March 2018, September 2018 and March 2019. Data from 25 244 patient visits included demographic variables, HbA1C, number of patient visits and insulin regimens.ResultsThere was no association between centre size and median HbA1C. On the other hand, there was a significant association between or median absolute deviation of HbA1C outcomes and the median HbA1C result between centres. On average every two thirds of a median absolute deviation increase in clinic HbA1C was associated with a 1.0% (10.9 mmol/mol) increase in median clinic HbA1C.ConclusionsOur data have shown that it is likely difficult for centres to have a low median HbA1C if there is high variance of HbA1C's within centres or within centre treatment groups. This appears to be true regardless of centre size. These findings need to be carefully considered by teams who wish to lower their clinic median HbA1C.
Thirty‐seven years of MT1 and MT2 melatonin receptor localization in the brain: Past and future challenges
Authors: Klosen Paul 
Published: April 2024

AbstractIdentifying the target cells of a hormone is a key step in understanding its function. Once the molecular nature of the receptors for a hormone has been established, researchers can use several techniques to detect these receptors. Here I will review the different tools used over the years to localize melatonin receptors and the problems associated with each of these techniques. The radioligand 2‐[125I] iodomelatonin was the first tool to allow localization of melatonin receptors on tissue sections. Once the MT1 and MT2 receptors were cloned, in situ hybridization could be used to detect the messenger RNA for these receptors. The deduced amino acid sequences for MT1 and MT2 receptors allowed the production of peptide immunogens to generate antibodies against the MT1 and MT2 receptors. Finally, transgenic reporters driven by the promoter elements of the MT1 and MT2 genes have been used to map the expression of MT1 and MT2 in the brain and the retina. Several issues have complicated the localization of melatonin receptors and the characterization of melatonin target cells over the last three decades. Melatonin receptors are expressed at low levels, leading to sensitivity issues for their detection. The second problem are specificity issues with antibodies directed against the MT1 and MT2 melatonin receptors. These receptors are G protein‐coupled receptors and many antibodies directed against such receptors have been shown to present similar problems concerning their specificity. Despite these specificity problems which start to be seriously addressed by recent studies, antibodies will be important tools in the future to identify and phenotype melatonin target cells. However, we will have to be more stringent than previously when establishing their specificity. The results obtained by these antibodies will have to be confronted and be coherent with results obtained by other techniques.
Technical challenges in REM sleep microstructure classification: A study of patients with REM sleep behaviour disorder
Authors: Yao C. William , Fiamingo Giuseppe , Lacourse Karine , Frenette Sonia , Postuma Ronald B. , Montplaisir Jacques Y. , Lina Jean‐Marc , Carrier Julie 
Published: April 2024

SummaryWhile commonly treated as a uniform state in practice, rapid eye movement sleep contains two distinct microstructures—phasic (presence of rapid eye movement) and tonic (no rapid eye movement). This study aims to identify technical challenges during rapid eye movement sleep microstructure visual classification in patients with rapid eye movement sleep behaviour disorder, and to propose solutions to enhance reliability between scorers. Fifty‐seven sleep recordings were randomly allocated into three subsequent batches (n = 10, 13 and 34) for scoring. To reduce single‐centre bias, we recruited three raters/scorers, with each trained from a different institution. Two raters independently scored each 30‐s rapid eye movement sleep into 10 × fSEM3‐s phasic/tonic microstructures based on the AASM guidelines. The third rater acted as an “arbitrator” to resolve opposite opinions persisting during the revision between batches. Besides interrater differences in artefact rejection rate, interrater variance frequently occurred due to transitioning between microstructures and moderate‐to‐severe muscular/electrode artefact interference. To enhance interrater agreement, a rapid eye movement scoring schematic graph was developed, incorporating proxy electrode use, filters and cut‐offs for microstructure transitioning. To assess potential effectiveness of the schematic graph proposed, raters were instructed to systematically apply it in scoring for the third batch. Of the 34 recordings, 27 reached a Cohen's kappa score above 0.8 (i.e. almost perfect agreement between raters), significantly improved from the prior batches (p = 0.0003, Kruskal–Wallis test). Our study illustrated potential solutions and guidance for challenges that may be encountered during rapid eye movement sleep microstructure classification.
Integration and the risk of liver cancer—Is there a real risk?
Authors: Kasimsetty Aradhana , Sabatino Denise E. 
Published: April 2024

AbstractAdeno‐associated virus (AAV)‐based gene therapies are in clinical development for haemophilia and other genetic diseases. Since the recombinant AAV genome primarily remains episomal, it provides the opportunity for long‐term expression in tissues that are not proliferating and reduces the safety concerns compared with integrating viral vectors. However, AAV integration events are detected at a low frequency. Preclinical studies in mouse models have reported hepatocellular carcinoma (HCC) after systemic AAV administration in some settings, though this has not been reported in large animal models. The risk of HCC or other cancers after AAV gene therapy in clinical studies thus remains theoretical. Potential risk factors for HCC after gene therapy are beginning to be elucidated through animal studies, but their relevance to human studies remains unknown. Studies to investigate the factors that may influence the risk of oncogenesis as well as detailed investigation of cases of cancer in AAV gene therapy patients will be important to define the potential risk of AAV genotoxicity.
Differences in shrimp pond bottom soil properties and bacterial load between acute hepatopancreatic necrosis disease (AHPND)‐infected ponds and AHPND‐free ponds and their relation to AHPND
Authors: Chainark Suwanit , Chainark Pitchaya , Soonsan Patcharee 
Published: April 2024

AbstractThis study investigated the disparities in soil characteristics and pathogenic bacteria prevalence between shrimp ponds affected by acute hepatopancreatic necrosis disease (AHPND) and unaffected ponds, alongside examining the spatial distribution of soil attributes in flat‐oriented pond soil strata. Using Pearson correlation and logistic regression analyses, relationships among variables and indicators associated with AHPND prevalence were discerned, leading to the formulation of a predictive model for AHPND occurrence. Soil samples were collected from distinct locations and depths within ponds across three southern provinces of Thailand's Andaman Seaboard. The analysis revealed significantly higher concentrations of several variables, including SOD, TIC, NO2−‐N, Ca, Mn, Cu, Zn, and specified Vibrio strains, in AHPND‐afflicted ponds, especially at 0–5 cm depth. A prominent differentiation was the escalated concentration of easily decomposable organic matter (EDOM) within infected ponds, implicating potential soil and water quality deterioration alongside heightened shrimp susceptibility to AHPND. Correlational analysis showed links between bacterial densities and organic matter groupings, trace elements, exchangeable bases, and soil pH, in AHPND‐infected ponds. The logistic regression model encapsulated three soil variables (TOC, Mg, and Mn) and one pathogen variable (V. parahaemolyticus) and furnished an equation to estimate the log (odds) of AHPND occurrence, facilitating better understanding and potential forecasting of AHPND prevalence in shrimp cultivation environments.
Highly structured populations of copepods at risk to deep‐sea mining: Integration of genomic data with demogenetic and biophysical modelling
Authors: Diaz‐Recio Lorenzo Coral , Tran Lu Y Adrien , Brunner Otis , Arbizu Pedro Martínez , Jollivet Didier , Laurent Stefan , Gollner Sabine 
Published: April 2024

AbstractCopepoda is the most abundant taxon in deep‐sea hydrothermal vents, where hard substrate is available. Despite the increasing interest in seafloor massive sulphides exploitation, there have been no population genomic studies conducted on vent meiofauna, which are known to contribute over 50% to metazoan biodiversity at vents. To bridge this knowledge gap, restriction‐site‐associated DNA sequencing, specifically 2b‐RADseq, was used to retrieve thousands of genome‐wide single‐nucleotide polymorphisms (SNPs) from abundant populations of the vent‐obligate copepod Stygiopontius lauensis from the Lau Basin. SNPs were used to investigate population structure, demographic histories and genotype–environment associations at a basin scale. Genetic analyses also helped to evaluate the suitability of tailored larval dispersal models and the parameterization of life‐history traits that better fit the population patterns observed in the genomic dataset for the target organism. Highly structured populations were observed on both spatial and temporal scales, with divergence of populations between the north, mid, and south of the basin estimated to have occurred after the creation of the major transform fault dividing the Australian and the Niuafo'ou tectonic plate (350 kya), with relatively recent secondary contact events (<20 kya). Larval dispersal models were able to predict the high levels of structure and the highly asymmetric northward low‐level gene flow observed in the genomic data. These results differ from most studies conducted on megafauna in the region, elucidating the need to incorporate smaller size when considering site prospecting for deep‐sea exploitation of seafloor massive sulphides, and the creation of area‐based management tools to protect areas at risk of local extinction, should mining occur.
Characterization of patients with aHUS and associated triggers or clinical conditions: A Global aHUS Registry analysis
Authors: Licht Christoph , Al‐Dakkak Imad , Anokhina Katerina , Isbel Nicole , Frémeaux‐Bacchi Véronique , Gilbert Rodney D. , Greenbaum Larry A. , Ariceta Gema , Ardissino Gianluigi , Schaefer Franz , Rondeau Eric 
Published: April 2024

AbstractIntroductionAtypical haemolytic uremic syndrome (aHUS) is a rare form of thrombotic microangiopathy (TMA) associated with complement dysregulation; aHUS may be associated with other ‘triggers’ or ‘clinical conditions’. This study aimed to characterize this patient population using data from the Global aHUS Registry, the largest collection of real‐world data on patients with aHUS.MethodsPatients enrolled in the Global aHUS Registry between April 2012 and June 2021 and with recorded aHUS‐associated triggers or clinical conditions prior/up to aHUS onset were analysed. aHUS was diagnosed by the treating physician. Data were classified by age at onset of aHUS (< or ≥18 years) and additionally by the presence/absence of identified pathogenic complement genetic variant(s) and/or anti‐complement factor H (CFH) antibodies. Genetically/immunologically untested patients were excluded.Results1947 patients were enrolled in the Global aHUS Registry by June 2021, and 349 (17.9%) met inclusion criteria. 307/349 patients (88.0%) had a single associated trigger or clinical condition and were included in the primary analysis. Malignancy was most common (58/307, 18.9%), followed by pregnancy and acute infections (both 53/307, 17.3%). Patients with an associated trigger or clinical condition were generally more likely to be adults at aHUS onset.ConclusionOur analysis suggests that aHUS‐associated triggers or clinical conditions may be organized into clinically relevant categories, and their presence does not exclude the concurrent presence of pathogenic complement genetic variants and/or anti‐CFH antibodies. Considering a diagnosis of aHUS with associated triggers or clinical conditions in patients presenting with TMA may allow faster and more appropriate treatment.image
Glucose breath test for the detection of small intestine bacterial overgrowth: Impact of diet prior to the test
Authors: Mattio Nastasia , Pradat Pierre , Machon Christelle , Mialon Anne , Roman Sabine , Cuerq Charlotte , Mion François 
Published: April 2024

AbstractBackgroundGlucose breath test (GBT) is used for the diagnosis of small intestine bacterial overgrowth. A restrictive diet without fibers and/or fermentable food is recommended on the day before the test. The aim of our retrospective study was to evaluate the impact of two different restrictive diets on the results of GBT.MethodsA change of the pretest restrictive diet was applied in our lab on September 1, 2020. The recommended diet was a fiber‐free diet before this date, and a fiber‐free diet plus restriction of all fermentable food afterward. We thus compared the results of GBT performed before (group A) and after (group B) this pretest diet modification. Demographics, reasons to perform GBT, digestive symptoms, and hydrogen and methane baseline values and variations after glucose ingestion were compared between the two groups.Key Results269 patients underwent GBT in group A, and 316 patients in group B. The two groups were comparable in terms of demographics. Methane and hydrogen baseline values were significantly higher in group A (respectively 14 [18] vs. 8 [14] ppm, p < 0.01 and 11 [14] vs. 6 [8] ppm, p < 0.01). The percentage of positive tests was higher in group A for methane (43% vs. 28%, p < 0.05), and for hydrogen (18% vs. 12%, p = 0.03).Conclusion & InferencesThis retrospective study suggests the importance of the restrictive diet prior to GBT. A strict limitation of fibers and fermentable food decreased hydrogen and methane baseline values, and the prevalence of positive GBT. Thus a strict restrictive diet should be recommended on the day before the test, in order to limit the impact of food on hydrogen and methane breath levels, and possibly improve the diagnosis quality of GBT.
Dissociative identity disorder in the dental setting: An assessment of the literature and case report
Authors: Pindoria Anushri , Radia Shenal , Stagnell Sami 
Published: April 2024

AbstractThere is an ever‐increasing chance that dentists are likely to experience patients who need additional management for psychiatric disorders. Dissociative identity disorder (DID) is characterised by the presence of two or more distinct personality states or identity fragments that control an individual's behaviour, thoughts and emotions. The aim is to offer additional insights into patients with DID and their management within a dental care setting. This will be demonstrated through the use of a case report as a practical illustration. In order to gain insight into the effects of DID on healthcare, particularly within a dental care environment, a comprehensive review of relevant literature has been conducted by searching, evaluating and synthesising existing academic and scholarly literature on DID. Additionally, the case reported is an example of how a 27‐year‐old patient with DID has been managed in an Intermediate Minor Oral Surgery setting. Patients often have their DID prompted by stress, as was the case with this patient. There are steps which can be taken in the dental setting to help reduce the patient's anxiety, understand their personalities and foresee any possible issues that could arise. In this case, intravenous sedation was used to extract the patient's wisdom teeth, and no other personalities presented during the procedure. The examination of existing literature along with a case report highlights the feasibility of effectively treating individuals with DID. This can be achieved through the careful recognition and handling of different personalities, triggers and potential challenges, all of which should be integrated into the treatment strategy.
Simple reflection exercises can build efficacy and reduce distress about relationship conflicts
Authors: Britton Emily M. , Marigold Denise C. , McGregor Ian 
Published: April 2024

AbstractSerious conflicts in close personal relationships can be highly distressing and tempting to ignore, but avoidance of conflict is maladaptive. In the present research, we tested the effectiveness of short conflict‐reflection interventions to promote constructive engagement with conflicts. In Study 1 (N = 358), a relatively unstructured, conflicted‐reflection intervention significantly reduced distress and bolstered confidence in partners' ability to resolve their relationship conflicts. Study 2 (N = 411) further revealed that this intervention was as, or nearly as effective as more elaborate interventions that prescribed specific, theory‐based, therapeutic elements. Together, results reveal that even brief episodes of constructive reflection on relationship conflicts can improve confidence and reduce distress about them. The positive effects of a relatively unstructured reflection suggest people already have adaptive intuitions about how to more effectively manage conflicts in their relationships and can benefit from brief reflections on how to apply them.