bluuebunny/crossref_metadata_2025_split huggingface dataset:
A New Framework for Enhancing VANETs through Layer 2 DLT Architectures with Multi-Party Threshold Key Management and PETs
Authors: Adarbah Haitham Y. , Kiraz Mehmet Sabir , Kardas Suleyman , Al-Bayatti Ali H. , Albayatti Hilal Mohammed Yousif 
Published: March 2024

Abstract
This study provides an architectural framework and a comprehensive examination of how the combination of threshold key management, privacy-enhancing technologies (e.g., homomorphic encryption, secure multi-party computation), and decentralised ledger technologies (DLT) enhances the security and privacy of vehicle ad-hoc networks (VANETs). We also examine the challenges of existing VANET structures, with a particular focus on trust, privacy, and scalability concerns. Our proposed architecture shifts from centralised to decentralised systems, highlighting the advantages of a decentralized ledger framework, specifically in ensuring the robustness, strong availability, integrity, and resilience of data against various cyber security threats. The architecture uses Layer 2s instead of Layer 1s as Layer 2s is much cheaper and faster. Furthermore, we explore how the aforementioned advanced cryptographic mechanisms are utilized in VANETs for improving key management, the distribution of trust, and data privacy, together with privacy-preserving data analysis, aiming to achieve resilient vehicle-to-everything (V2X) communication and privacy-preserving data analysis. We conclude by highlighting potential future directions for more secure, efficient, and resilient VANET systems in the era of 5G and beyond.
Germinal Center Dark Zone harbors ATR-dependent determinants of T-cell exclusion that are also identified in aggressive lymphoma
Authors: Tripodo Claudio , Cancila Valeria , Morello Gaia , Bertolazzi Giorgio , Chan Allison Si-Yu , Bastianello Giulia , Paysan Daniel , Jaynes Patrick William , Schiavoni Giovanna , Mattei Fabrizio , Piconese Silvia , Revuelta Maria , Noto Francesco , De Ninno Adele , Cammarata Ilenia , Pagni Fabio , Venkatachalapathy Saradha , Sangaletti Sabina , Napoli Arianna Di , Vacca Davide , Lonardi Silvia , Lorenzi Luisa , Ferreri Andrés J M , Belmonte Beatrice , Varano Gabriele , Colombo Mario Paolo , Bicciato Silvio , Inghirami Giorgio , Cerchietti Leandro , Ponzoni Maurilio , Zappasodi Roberta , Facchetti Fabio , Foiani Marco , Casola Stefano , Jeyasekharan Anand D 
Published: March 2024

Abstract
The germinal center (GC) dark zone (DZ) and light zone (LZ) regions spatially separate expansion and diversification from selection of antigen-specific B-cells to ensure antibody affinity maturation and B cell memory. The DZ and LZ differ significantly in their immune composition despite the lack of a physical barrier, yet the determinants of this polarization are poorly understood. This study provides novel insights into signals controlling asymmetric T-cell distribution between DZ and LZ regions. We identify spatially-resolved DNA damage response and chromatin compaction molecular features that underlie DZ T-cell exclusion. The DZ spatial transcriptional signature linked to T-cell immune evasion clustered aggressive Diffuse Large B-cell Lymphomas (DLBCL) for differential T cell infiltration. We reveal the dependence of the DZ transcriptional core signature on the ATR kinase and dissect its role in restraining inflammatory responses contributing to establishing an immune-repulsive imprint in DLBCL. These insights may guide ATR-focused treatment strategies bolstering immunotherapy in tumors marked by DZ transcriptional and chromatin-associated features.
What matters for the efficiency of Micro Business Tax Collection? Evidence from the Eastern Part of Ethiopia.
Authors: enate Anwar Adem Shikur , Slih Salih Ülev , sis Sisay Redwan Ababiya 
Published: March 2024

Abstract
This study aims to identify factors that influence efficiency of micro Business tax collection in the Easter part of Ethiopia. A ordered logistic regression model was used to analyze the efficiency of micro business tax collection and taxpayer tax knowledge, tax law enforcement, corruption, tax compliance resolution, and fairness of tax through a closed questionnaire by using stratified sampling technique. The study results show that efficiency of micro business tax collection in the eastern part of Ethiopia are influenced by the taxpayer tax knowledge, tax law enforcement, corruption control, and the tax compliance resolution capacity of the tax authorityThe study's findings will provide insights into improving the efficiency of microbusiness tax collection in Ethiopia from the perspective of microbusiness taxpayers. The final results of this study are distinctive because they show how the efficiency of microbusiness tax collection in Ethiopia is affected by how much taxpayers know about taxes, how well tax laws are enforced, how well corruption is controlled, and how well the tax authority can solve problems with tax compliance.
Environmental DNA from ethanol eluent of flowers reveals a widespread diversity in cowpea associated animal communities in Hainan Island
Authors: Chen Qi , Yu Huai-Liang , Lv Jun-Xian , Wang Xing , Li Jin , Wu Ming-Yue , Shi Cai-Hua , Xie Wen , Kong Xiang-Yi , Huang Guo-Hua 
Published: March 2024

Abstract
Cowpea (Vigna unguiculata (L.) Walp.), as an economical crop, is one of the important pillar industries of rural revitalization strategy in China. However, cowpea planting in China is often infested and damaged by many insects during growth, especially in Hainan region with a warm and wet tropical climate. Traditional monitoring methods with technical limitation could only detect a few common significant agricultural pests, how many kinds of species associated with cowpea is unknown. Here, we employed environmental DNA (eDNA) metabarcoding to characterize cowpea associated animal community-level diversity among six planting areas in Hainan. In all, 62 species were detected, of which 99.05% was Arthropoda, suggesting that Arthropods are the main groups interacting with cowpea. Moreover, we also detected 28 pests on cowpea, predominantly belonging to Thysanoptera, Lepidoptera, Diptera and Hemiptera, of which 20 pests were first reported and need more extra attention. Furthermore, clustering results indicated that there is a certain diversity of cowpea associated animals in different regions of Hainan, but the species composition was similar in the large planting areas due to the indiscriminate use of pesticides, which need further develop scientific pesticide applications to ensure adequate species diversity. This study represents the first molecular approach to investigate the cowpea associated animal communities and provides basic information for further scientific pesticide applications.
Ranking of Cloud Services by applying BWM-TOPSIS, BWM-ARAS, and BWM-COPRAS Hybrid MCDM Methods
Authors: Mishra Anupama , Kumar Rakesh 
Published: March 2024

Abstract
The cloud computing business is a global industry with a multitude of cloud service providers. Customers may utilize the MCDM approach to appraise and analyze cloud service providers (CSPs) according to their requirements. This study presents a proposal for utilizing hybrid Multiple Criteria Decision Making (MCDM) methodologies. A hybrid Multiple Criteria Decision Making (MCDM) technique has been created to evaluate and rank cloud service providers using use-case scenarios. The hybrid approach has two components. Initially, we employ the Best Worst Method (BWM) weighting estimate technique to compute the criterion weights and relative scores of CSPs. Next, we utilize the TOPSIS, ARAS, and COPRAS techniques to evaluate and rank the cloud service provider. We illustrate the effectiveness and capabilities of hybrid approaches for evaluating cloud service providers through a use-case scenario. The proposed methods are compared, and it is found that outcomes are more or less the same, and ranking results of CSPs are almost identical using the different quantity of criteria and CSPs. We compared the recommended methods to commonly used AHP, and results indicate that suggested approach is more efficient than AHP. The suggested method requires fewer pairwise comparisons compared to AHP, which uses a different set of criteria.
Willow Algorithm for Consumption-Investment under Stochastic Volatility Model with Jump Diffusion
Authors: Wang Kunlun , Xu Wei , Ma Junmei , You Pei 
Published: March 2024

Abstract
We mainly focus on the willow algorithm which can be used to solve the optimal dynamic multi-cycle investment and consumption problem under the jump-diffusion stochastic volatility model. First we obtain the moment generating function of the risky asset and then coalescing the Johnson-Curve transformation theory to generate the willow algorithm, which solving the multi-cycle dynamic investment and consumption problem is designed based on the two-dimensional willow framework. Moreover, through comparing our proposed solution with the optimal investment and consumption display solutions under the geometric Brownian motion model, we further discuss the analysis of the willow algorithm sensitivity. The willow algorithm for optimal investment and consumption proposed in this paper is able to extend the willow method which is effective from the field of option pricing to the field of investment portfolio, and it also provides a new idea for numerically solving the multi-period optimal investment and consumption decision.
Effects of Aquatic Exercises on Mental Health, Functional Autonomy and Muscle Efficiency in Elderly With Multimorbidity: a Randomized Clinical Trial
Authors: Silva Vitória Oliveira Silva da , Santos Mauricio Fagundes , Macedo Rafael Alex dos Santos , Giustina Lucas Lacerda Della , Lass Andre Domingos , de Pinho Ricardo Aurino , Hoerpers Neiva Junkes , Martins Karin Gomes , Silveira Paulo Cesar Lock , Silva Luciano Acordi da 
Published: March 2024

Abstract

Background
Aquatic exercises have been used for centuries to prevent and treat various diseases, improving the quality of life in the elderly. The present study investigated the effects of high-intensity interval aquatic exercise (Hydro-HIIT) on mental health parameters, functional autonomy, and muscular efficiency in elderly people with multimorbidity.
Methods
A total of 129 subjects were included in this randomized clinical study and were assigned to G1 (Hydro-HIIT) 1x per week (n = 24) and G2 (Hydro-HIIT) 2x per week (n = 39) for 12 weeks. All tests were performed 48 hours before the program started and 48 hours after its last session.
Results
After intervention, G2 showed reduction in sleepiness (-50%), anxiety (-45%) and depression (-62%), and improved markers in sleeping (+ 49%), standing and sitting autonomy (+ 37%), getting wup e walking (+ 38%), stationary march (+ 17%), arm flexion (+ 45%), leg/arm muscle endurance (+ 18%), and leg strength (+ 19%) (p < 0.05).
Conclusions
Hydro-HIIT twice a week improves mental health parameters, functional autonomy, and muscular efficiency in the elderly with multimorbidity.
Exploring Emotion Control and Alexithymia in Autistic Adults: An Ecological Momentary Assessment Study
Authors: Costache Mădălina Elena , Gioia Federica , Vanello Nicola , Greco Alberto , Lefebvre François , Capobianco Antonio , Weibel Sébastien , Weiner Luisa 
Published: March 2024

Abstract
Background

Difficulties in controlling emotions – a proxy for emotion dysregulation (ED) - and difficulties in expressing feelings in words - ‘absence of emotion labelling’ - co-exist in autism and contribute to elevated levels of impulsive and suicidal behaviour. To date, studies linking the two phenomena have relied on retrospective self-reported measures, lacking support for generalizability to real-life situations.

Methods

The present study investigated in vivo emotion labelling and its impact on emotion control in 29 autistic adults without intellectual disability (ASC) and 28 neurotypical (NT) individuals of similar age, sex, and educational level. Participants were trained in an Ecological Momentary Assessment (EMA) to label their emotions, the arousal dimension, and their emotion control via smartphone over a one-week period.

Results

Findings showed that the ASC group experienced more instances of ‘having an emotion that I cannot name’ and, when they were able to label their emotions, they reported higher rates of negative and conflicting (simultaneously positive and negative) emotions. In both groups, the absence of emotion labelling, and intense negative emotions were associated with impaired emotion control. However, the association between lack of emotional awareness - ‘I have no emotion’ - and impaired emotion control was only evident in ASC individuals. This effect persisted after controlling for demographics, co-occurring clinical symptoms or autism severity.

Limitations

Concepts such as ‘emotion control’, referring to the ability to regulate emotions, and ‘I have an emotion that I cannot name’ and ‘I have no emotion’, indicating difficulty or lack of awareness of emotions, have not been previously validated. This study does not address the correspondence between self-report measures and physiological indicators.

Conclusions

Despite previous research suggesting associations between ED and co-occurring psychiatric conditions or autistic traits, our study found that even after controlling for these factors, the only significant predictors of reduced emotion control were the absence of emotion labelling and intense negative emotions. Further research is needed to gain a deeper understanding of this complex relationship.
Study of loss of the active principle capecitabine and cytotoxic activity in preparation intended for administration in liquid form
Authors: Junior Eliezer C. Coelho , Daltoé Renata D. , Madeira Klesia P. , Paula Heberth , Severi Juliana A. 
Published: March 2024

Abstract
Purpose
Despite the advantages for oral administration, some patients may encounter difficulty swallowing the Capecitabine (CAP) tablet, leading to its administration in the form of a solution prepared from crushed and dissolved tablets in water, thus constituting an off-label use. It was analyzed whether, from the dissolution of the tablet in water, there is a loss of the active ingredient and, consequently, a decrease in its cytotoxic effect.
Methods
The quantification of the active ingredient was carried out using Ultra Fast Liquid Chromatography, and the assessment of the cytotoxic effect of the solution was conducted using the MTT assay in breast cancer cell lines MCF-7 and MDA-MB-231. Additionally, the activity of the Thymidine phosphorylase enzyme was determined in the same cell lines by measuring the consumption of the substrate thymidine using the supernatant of the cell lysates through spectrophotometry.
Results
There was no significant change in the concentration of the active ingredient CAP in the solution prepared for up to 6 hours. A significant cytotoxic effect was observed after treatment in the cell lines, suggesting preserved cytotoxicity for at least 120 minutes after preparation. The activity of the Thymidine phosphorylase enzyme in the MDA-MB-231 cell line is 26.6% higher compared to the MCF-7 cell line.
Conclusion
It is suggested the safe use of the off-label form of CAP, adding greater treatment possibilities for patients with MBC. It is also suggested that the metabolic pathway for converting CAP to 5-FU may not be solely dependent on hepatic enzymes.
Nature’s solution to degrade long-chain hydrocarbons: A life cycle study of beeswax and plastic eating insect larvae
Authors: Kundungal Harsha , Devipriya Suja P. 
Published: March 2024

Abstract

Recent studies reported biodegradation of polyethylene waste in the gut of beeswax eating insect larvae of Galleria mellonella and Achroia grisella. In this study we examined the lifecycle stages and duration of each stage of three common beeswax eating honey bee pests (Galleria mellonella, Achroia grisella and, Uloma sp.) of Apis cerana indica colonies in laboratory condition. The insect larvae of the three species were provided with beeswax, polyethylene (PE) and polystyrene (PS) diet. The weight loss of beeswax, PE and PS feed as a function of time caused by the three insect larvae consumption was measured. Also the effect of beeswax, PE and PS feed on the growth and survivability of the three insect larvae were studied. Beeswax feeding insects G. mellonella, A. grisella, and Uloma sp. completed their lifecycle and the average lifecycle duration was 62 ± 2.1, 49 ± 3.6, 202 ± 3.2 days respectively. The G. mellonella A. grisella, and Uloma sp. larvae eat and digest beeswax, PE and PS. The insect larvae were found to survive on the plastic diets (PE and PS) but a decreased body mass was observed compared to beeswax eating conspecifics. These insect larvae that eat and digest beeswax and plastics could help to eliminate global pollution from recalcitrant plastic wastes.
Huangjing (Polygonatum kingianum Coll. et Hems) and Tiandong (Asparagus cochinchinensis (Lour.) Merr.) Combination relieves glycolipid metabolism disorder via both Glycolipid key Proteins and gut microbiota
Authors: Li Jie , Tao Yating , Xia Jinfeng , Zhou Lianli , Wang Wenbo , Tang Ruidan , Yang Xingxin , Chen Zhen , Zhang Fan , Yu Jie 
Published: March 2024

Abstract
Background
Ancient traditional Chinese medicine (TCM) books and modern studies have suggested that the Huangjing and Tiandong combination (HTC), which consists of Polygonatum kingianum (PK) and Asparagus cochinchinensis (AC) with the mass ratio 10:3, has the effect of regulating glycolipid metabolism disorder. However, its efficacy and mechanism are yet to be elucidated. This research evaluates the effect of HTC on glycolipid metabolism and explores the underlying mechanism.
Methods
GLMD was induced by HFSD diet in rats, and Huangjing and Tiandong combination (HTC) was given orally every day for 12 weeks. Then the body weight, tissue weight, blood sugar, blood lipid and liver lipid levels were measured, and lipogenesis was evaluated by Oil-Red O staining. In addition, the role of potential key signaling pathway was investigated through a network pharmacology-guided ELISA, while the gut microbiota was studied via Metagenome and 16S rDNA sequencing.
Results
Oil red O staining showed that HFSD diet led to a significant accumulation of lipid droplets in the liver. After HTC extract treatment, lipid droplets decreased, and normal morphology of the liver was gradually restored. Besides, the results showed that HTC could improve blood glucose, blood lipids, adipokines and liver lipids. Then, we found that HTC could increase the expression of INSR, IRS1, IRS2, PI3K, Akt, JAK2, and STAT3 in liver, and abdominal white adipose tissue. Subsequently, we reported that HTC exhibited beneficial effects against GLMD via the alternation of the abundance and diversity of gut microbiota.
Conclusion
In a word, the above results demonstrate that HTC is a useful drug/nutrient to relieve GLMD via both Glycolipid key Proteins and gut microbiota.
Recurrence of Rectal Cancer on the Pelvic Sidewall After Lateral Lymph Node Dissection
Authors: Takao Misato , Kawai Kazushige , Nakano Daisuke , dejima Akira , Nakamori Sakiko , Natsume Soichiro , Ise Ichiro , Kato Hiroki , Yamaguchi Tatsuro 
Published: March 2024

Abstract

Purpose
Although lateral lymph node dissection has been performed to prevent lateral pelvic recurrence in locally advanced lower rectal cancer, the incidence of lateral pelvic recurrence after this procedure has not been investigated. Therefore, this study aimed to investigate the long-term outcomes of patients who underwent lateral pelvic lymph node dissection, with a particular focus on recurrence patterns.
Methods
This was a retrospective study conducted at a single high-volume cancer center in Japan. A total of 493 consecutive patients with stage II-III rectal cancer who underwent lateral lymph node dissection between January 2005 and August 2022 were included. The primary outcome measures included patterns of recurrence, overall survival, and relapse-free survival. Patterns of recurrence were categorized as lateral or central pelvic.
Results
Among patients who underwent lateral lymph node dissection, 18.1% had pathologically positive lateral lymph node metastasis. Lateral pelvic recurrence occurred in 5.5% of patients after surgery. Multivariate analysis identified age > 75 years, lateral lymph node metastasis, and adjuvant chemotherapy as independent risk factors for lateral pelvic recurrence. Evaluation of the recurrence rate by dissection area revealed approximately 1% of recurrences in each area after dissection.
Conclusion
We demonstrated the benefits and limitations of lateral lymph node dissection for patients with advanced lower rectal cancer, focusing on the incidence of recurrence in the lateral area after the dissection. Our study emphasizes the clinical importance of lateral lymph node dissection, which is an essential technique that surgeons should acquire, even in this era of chemoradiotherapy.
Australian Senecio macrocarpus and S. squarrosus were suggested as apomictic but are fully sexual: evidence from flow cytometric seed screening analyses
Authors: Mráz Patrik , Ahrens Collin W. , James Elizabeth A. 
Published: March 2024

Abstract

Asexual seed reproduction, apomixis, is a relatively rare reproductive strategy in angiosperms. Its rarity is probably due to evolutionary constraints that require full and synchronous expression of all the steps that modify the ancestral sexual pathway. Therefore, the documentation of any new occurrence of apomixis, especially in taxonomic groups with no previous report, should be based on thorough and solid evidence.
Here we correct the report of apomictic reproduction in two endemic hexaploid species of Senecio from Australia. Based on either excess or deficiency of expected heterozygosity across loci, over-representation of several multilocus genotypes, and segregation analysis assessed using microsatellite markers, it was suggested that apomixis together with selfing could explain the observed patterns of genetic variation in perennial S. macrocarpus and annual S. squarrosus.
We used flow cytometric seed screening analyses to verify the mode of reproduction of several widespread multilocus genotypes that are considered to be apomictic. These analyses revealed that all seed progeny arose by the sexual pathway, as they exhibited a constant 3:2 endosperm to embryo ploidy ratio, typical of double fertilization of a reduced embryo sac by two reduced sperm cells. Therefore, both species reproduce sexually. We suggest that the unusual genetic patterns in both allopolyploids are caused by originally low variation in the parental species, which probably also reproduce by selfing, disomic inheritance and probably also the presence of null alleles. We call for a more careful and critical interpretation of the patterns obtained, taking into account all aspects related to apomictic reproduction.
The Relationship Between Cooking and Food Preparation Skills of Mothers with Children Diagnosed with Autism Spectrum Disorder and Child's Eating Behaviors and Gastrointestinal Symptoms
Authors: KOÇ Fatma , YILMAZ Hacı Ömer 
Published: March 2024

Abstract
Mothers have an important role in feeding children. The aim of this study was to determine the relationship between the cooking and food preparation skills of mothers with children diagnosed with autism spectrum disorder and the child's eating behaviors and gastrointestinal symptoms. A significant negative correlation was found between the mothers' total scores on the cooking and food preparation skills and their children's eating behaviors and gastrointestinal symptoms scores. In addition, it was determined that mothers with low scores on the cooking and food preparation skills experienced more eating problems such as food refusal and autism-specific behaviors and gastrointestinal symptoms such as abdominal pain, reflux, diarrhea, indigestion and constipation in their children with autism. It was found that there was a relationship between food preparation and cooking skills of mothers and eating behaviors and gastrointestinal symptoms of their children with autism.