bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transformation cellulaire maligne et modifications caryologiques: Apport de l'hybridation somatique de lignées de hamster Chinois malignant
Authors: Berebbi M. , Meyer G. 
Published: February 2007

AbstractNotre étude a porté sur différents clones isolés a partir d'une lignte cellulaire hybride obtenue par fusion de deux sub‐lignées de hamster chinois ayant une origine commune, mais des caractères distincts et, en particulier, des caractéresistiques de transplantabilité. Afin d'ktudier les propriktks de la transformation cellulaire, cette population hybride htttrogkne, decrite par Berebbi et Barski (1971) a été clonée en gélose semi‐molle. Trois critères de la trans formation (eficacité clonale, agglutinabilité à la Concanavaline A et tumorigénicité) ont été étudiés. En eflet, la souche DC‐3F qui est transformie spontanément in vitro a un pouvoir tumorigène maximum, un coeficient d'agglutinabilitk élevé et un rendement en colonie aussi très élevé. Par contre, la souche parentale qui en derive (DC‐3F/A DX/Aza) a perdu son pouvoir tumorigène, agglutine beaucoup plus dificilement et ne nous a pas donné de colonies en gtlose. Or, l'étude de la population hybride de départ nous montre des résultats intemédiaires aussi bien pour la croissance en gélose que pour l'agglutinabilité à la Concanavaline A. De méme, les différents clones issus de la population hybride de départ nous montrent des différences dans le pouvoir tumorigéne et celui‐ci semble encore lié aux modifications des propriétés de surface. En effet, ces clones n'ont pas la méme efficacité clonale en gélose et n'agglu‐tinent pas de la měme maniére. Nous les avons séparés en deux parties, les clones 2 et A qui agglutinent faiblement et les clones 3 et Ebl qui ont un coefficient d'agglutinabilité beaucoup plus élevé. Nous avons étudié en paralléle l'évolution du caryotype de la population hybride in vitro des clones et des tumeurs correspondantes, et nous avons essayé de faire une analyse chromosomique statistique pour chaque souche. C'est ainsi que nous avons essayé d'établir une corrélation entre l'apparition d'un chromosome subtélocentrique, marqueur de l'hybride (la moyenne arithmétique pour ce marqueur étant de 0.85) et le pouvoir tumorigéne. Or, cette moyenne arithmétique du marqueur M2 varie tout au cours des subcultures de l'HyC et dans les différents clones étudiés. II semble donc y avoir une relation entre la présence de ce marqueur et le pourcentage de tumeurs obtenues. Cependant, d'aprés l'etude statistique comparée de chacun des clones et des tumeurs issues de ces clones, le marqueur M2 ne semble pas le seul responsable de la malignité car l'équilibre des autres paires chromosomiques est impliqué dans l'expression de la transformation cellulaire, le marqueur M2 semblant plus spécialement concerné dans les modifications de surface cellulaire. Ce phénoméne polygénique de la transformation cellulaire est donc moins le résultat d'une dominance ou d'une délétion que d'un désquilibre génétique.
The ontogeny of alpha‐foetoprotein in the foetal and neonatal rabbit, and its experimental induction in adult rabbits
Authors: Branch W. R. 
Published: February 2007

AbstractThe relative concentration of AFP in the foetal and neonatal rabbit was studied by immunological methods. A peak foetal concentration of AFP was found between the 24th and 26th days of gestation. AFP could not be detected in newborn serum after the 28th day post‐partum. The concentration of AFP between the 20th and 25th days of gestation was found to rise from 327 mg/100 ml to 525 mg/100 ml.The synthesis of AFP in young and adult rabbits was successfully induced (a) during liver regeneration following carbon tetrachloride intoxication in rabbits less than 14 weeks of age, and (b) following the induction of hepatomas by oral administration of N.nitrosodiethylamine.
Rescue of the murine sarcoma virus genome from non‐producer cells by the RD‐114 type C virus
Authors: Gilden R. V. , Lee Y. K. , Long C. 
Published: February 2007

AbstractA pseudotype focus‐forming virus was prepared by fusion of human cells infected with RD‐114 virus and non‐producer hamster cells transformed by murine sarcoma virus. The focus‐forming virus, designated MSV (RD‐114), transformed human fibroblasts, including WI‐38, but no mouse, hamster, or rat embryo cells. MSV (RD‐114) possesses envelope, group‐specific, and polymerase antigenic reactivities characteristic of RD‐114 virus. RD‐114 infected cells were resistant to focus formation by MSV (RD‐114) while no diminution in focus‐forming activity was observed in cells infected with feline leukemia virus.
Radioimmunoassay of alpha‐fetoprotein: A contribution to the diagnosis of choriocarcinoma and hydatidiform mole
Authors: Seppälä M. , Bagshawe K. D. , Ruoslahti E. 
Published: February 2007

AbstractDetermination of the circulating alpha fetoprotein (AFP) can be used to differentiate between choriocarcinoma or hydatidiform mole and normal pregnancy. Whilst a positive pregnancy test is common to all these states, the serum AFP was found to be elevated only when a fetus was present.
The effect of long‐term exposure to DDT on CF‐1 mice
Authors: Tomatis L. , Turusov V. , Day N. , Charles R. T. 
Published: February 2007

AbstractCF‐1 minimal inbred mice were given technical DDT mixed into the diet at the dose levels of 2, 10, 50 and 250 parts per million (ppm) for the entire life span for two consecutive generations. Exposure to all four levels of DDT resulted in a significant increase of liver tumours in males, this being most evident at the highest level used. In females, the incidence of liver tumours was slightly increased following the exposure to 10 and 50 ppm, while a marked increase was observed following exposure to 250 ppm. In DDT‐treated animals the liver tumours were observed at an earlier age than in untreated controls. The age at death with liver tumours and the incidence of liver tumours appeared to be directly related to the dose of DDT to which the mice were exposed. Four liver tumours, all occurring in DDT‐treated mice, gave metastases. Histologically, liver tumours were either well‐differentiated nodular growths, pressing but not infiltrating the surrounding parenchyma, or nodular growths in which the architecture of the liver was obliterated showing glandular or trabecular patterns.
Inhibition of established transplants of chemically induced sarcomas in syngeneic mice by lymphocytes from immunized donors
Authors: Borberg H. , Oettgen H. F. , Choudry K. , Beattie E. J. 
Published: February 2007

AbstractIntravenous injections of lymph node cells from immunized BALB/c and (C57BL/‐6 X A) F1 mice and from immunized sheep induced regression or inhibition of established grafts of a methylcholanthrene‐induced sarcoma in syngeneic BALB/c mice.
Transplantable immunoglobulin‐secreting tumours in rats. I. General features of LOU/Ws1 strain rat immunocytomas and their monoclonal proteins
Authors: Bazin H. , Deckers C. , Beckers A. , Heremans J. F. 
Published: February 2007

AbstractThis article describes a peculiar tumour which originates in the ileocoecal lymph node of the LOU/Ws1 strain rat. The tumour cells usually have the appearance of very poorly differentiated lymphoid cells showing a tendency to develop ribosome‐studded ergastoplasmic membranes. In about 60% of the cases, such tumours synthesize monoclonal immunoglobulins of the IgG or IgA class, or Bence‐Jones proteins. These tumours are readily transplantable in isologous hosts or their F hybrids. The great majority of the transplanted growths retain their capacity for immunoglobulin production during many successive passages in vivo.A scrutiny of older and more recent literature has revealed several descriptions of rat tumours having the same macroscopic and microscopic features as the tumours described here, except for the immunoglobulin‐secreting properties which were never tested in the earlier studies.It is proposed to call this tumour the rat ileocoecal immunocytoma.
Cell‐mediated reaction against tumors induced by oncornaviruses. I. Kinetics and specificity of the immune response in Murine sarcoma virus (MSV)‐induced tumors and transplanted lymphomas
Authors: Leclerc J. C. , Gomard E. , Levy J. P. 
Published: February 2007

AbstractAnti‐tumour cell‐mediated immune reactions were studied by the chromium release test (CRT) in mice bearing autochtonous Murine Sarcoma Virus (MSV)‐induced sarcomas or syngeneic transplants of lymphomas with various antigenic specificities. Cytotoxic lymphoid cells were detected during tumor growth, but disappeared rapidly after day 13–15 in “progressors”, and more slowly in mice which were able to reject the tumor (“regressors”). The level of cytolytic activity of lymphoid cells in the spleen of “regressors” did not parallel the immune protection. No blocking of the cytotoxic activity of lymphoid cells was detected in CRT using either “progressor”, or “regressor” sera. The discrepancies which exist between these results and those which were obtained in the same tumor system by colony inhibition test or microcytotoxicity assay may suggest that different phenomena, possibly involving different lymphoid cell populations, were studied by the various methods. The antigenic specificity of the immune reactions which were evidenced by the CRT was not identical to those revealed by serological methods, cross‐reactivity being regularly observed in CRT between FMRGi+ and G+lymphomas.
The surface structure of murine ascites tumors. II. Difference in cytotoxicity of various phytoagglutinins toward yoshida sarcoma cells in vitro
Authors: Tomita Motowo , Kurokawa Tsutoma , Onozaki Kikuo , Osawa Toshiaki , Sakurai Yoshio , Ukita Tyunosin 
Published: February 2007

AbstractSeveral phytoagglutinins having different sugar specificities were found to exert different agglutinating activity toward murine ascites cell lines. It was also found that some of these phytoagglutinins, especially.Ricinus communis.and.Abrus precatorius.agglutinins, inhibited the growth of Yoshida sarcoma (YS) cells and showed a strong cytotoxic effect. This kind of cytotoxicity of.Ricinus communis.agglutinin, which is specific for galactose, was shown to be counteracted by the addition of galactose but not of glucose, and this fact indicated that the cytotoxicity of the phytoagglutinin is related to the binding specificity of the agglutinin toward the sugar chains on the cell surface. On the other hand, in some phytoagglutinins, the cytotoxicity was found to be relatively low even when their agglutinating activity was remarkably high. On the basis of these results, the role of the sugar chains on cell surface in cellular functions is discussed.
Properties of an SV40‐transformed hamster cell line resistant to actinomycin D
Authors: Wicker R. , Bourali M.‐F. , Suarez H. G. , Cassingena R. 
Published: February 2007

AbstractThe biological properties of a hamster cell line Cl 2 TSV‐5/R derived from an SV40 tumour and made resistant to Actinomycin D (2 μg/ml) were investigated, in particular: tumorigenicity in vitro growth on plastic or in soft agar, agar, persistence of SV40 specific neoantigens (T and TSTA), and caryotype. When compared to the parental cell line (Cl 2 TSV‐5), the tumorigenicity and the ability to grow in soft agar of the Cl 2 TSV‐5/R cells were strongly reduced (approximately 1,000 fold). Their growth on plastic was also clearly slowed. Although the SV40 T antigen was still present in all the resistant cells they no longer had immunosensitivity to the SV40 specific graft rejection test. The caryotype of Cl 2 TSV‐5/R cells was comparable to that of the Cl 2 TSV‐5 cells.
Growth and antigenic properties of a biopsy‐derived Burkitt's lymphoma in thymus‐less (nude) mice
Authors: Povlsen C. O. , Fialkow P. J. , Klein Eva , Klein G. , Rygaard J. , Wiener F. 
Published: February 2007

AbstractA Burkitt's lymphoma was transferred to the congenitally athymic mouse mutant nude with biopsy material from a 7‐year‐old Kenyan girl. The tumor grows locally at the site of inoculation with no distant metastases. The established tumor has been maintained for six passages so far with preservation of histological and cytological appearance. The mouse‐passaged tumor has a normal human diploid female chromosome complement. Isozyme studies have shown tumor to be of the same glucose‐6‐phosphatedehydrogenase (G‐6‐PD) and phosphoglucomutase1 phenotype (B and 2‐1 respectively) as tumor biopsy from the patient. The mouse‐passaged line maintained surface IgM, similarly to the original tumor and the derived tissue culture line, but lost the IgG coating characteristic for the original tumor but absent from two subsequent biopsies and from the derived tissue culture line. This is in line with previous observations indicating that surface‐associated IgG on Burkitt biopsies is due to coating from the outside, whereas surface‐associated IgM is a cell marker. Whereas the biopsy cells of the patient were positive for EBV‐associated membrane antigen (MA), but not for early antigen (EA) and viral capsid antigen (VCA), the mouse‐passaged line was positive for all three. This suggests that the restrictive influence of the human host on the production of EA and VCA in the Burkitt tumor is raised in the mouse host. The serum of the tumor‐bearing nude mice contained anti‐human antibodies, but no detectable EBV (anti‐MA, EA and VCA)‐antibodies.
Cell locomotion and contact inhibition of normal and neoplastic rat cells
Authors: Veselý Pavel , Weiss Robin A. 
Published: February 2007

AbstractThe morphology, locomotion and contact inhibition of locomotion of normal and neoplastic rat cells were studied in relation to oncogenicity. Embryonic fibroblasts (LWF) and a spontaneously neoplastic variant (LW13) were both fibroblastic in appearance, with a monolayered arrangement of cells at moderate densities. A variant transformed by Rous sarcoma virus (RsK4) was epithelioid and was also well monolayered. Scanning electron microscopy revealed a smooth dorsal surface on LWF cells, microvilli on the dorsal surface of LW13 cells and numerous microvilli, blebs and ruffles over the entire surface of RsK4 cells. LWF cells and LW13 cells moved with broad fan‐shaped lamellae on which lamellipodia (ruffles) arose at the leading edge. These cells showed polarized locomotion and migrated rapidly into wounds made in confluent cultures. RsK4 cells neither possessed large, fan‐shaped lamellae nor showed polarized movement. They did not invade wounds efficiently. After treatment with colcemid LWF and LW13 cells resembled RsK4 cells more closely and lost polarity of movement. On analysis of cell collisions by time‐lapse cinemicrography, the three cell types showed pronounced homotypic contact inhibition of cell locomotion, as judged by cessation of forward displacement. LWF and LW13 cells exhibited paralysis of ruffling, contraction of the leading edge, cell to cell adhesion and inhibition of pinocytosis typical of contact inhibition between fibroblasts. Paralysis and contraction were not evident in RsK4 cell collisions. Colcemid treatment did not prevent the initiation of contact inhibition but reduced the lasting paralysis of LWF and LW13 cells. In collisions between tumour cells and normal cells, cell locomotion and membrane ruffling were inhibited in the normal cells but not in the tumour cells. Such non‐reciprocal contact inhibition resulted in apparent monolayering in mixed cultures. The loss of contact inhibitory responses of neoplastic cells to normal cells appears to be more closely related to oncogenicity than are homotypic contact inhibition and cellular morphology. The contact inhibitory stimuli of certain neoplastic cells differ from those of normal fibroblasts, and may be preserved by fixation with glutaraldehyde.
Synthesis of murine leukemia virus in cell culture
Authors: Okano Hiromitsu , Rich Marvin A. , Johns Lewis , Rich Ruth 
Published: February 2007

AbstractUsing high resolution, electron microscopic autoradiography, the times for macromolecular synthesis and assembly of a murine leukemia virus were determined. The time between completion of viral, RNA and protein synthesis and the appearance of morphologically complete virions did not exceed 30 min. The sequence of cellular labeling following exposure to 3H‐uridine was: nucleolar; nuclear; cytoplasmic. Labelling kinetics for viral RNA paralleled that for cytoplasmic RNA and coincided with a decrease in grain counts over nucleolar areas. Comparison of labelling kinetics for type‐A and ‐C virions supports the idea the type‐A virions are precursors for type‐C.
Cytogenetic analysis of human acute and chronic myeloid leukemic cells cloned in agar culture
Authors: Moore Malcolm A. S. , Metcalf Donald 
Published: February 2007

AbstractKaryotypic analysis was performed on agar cultures of blood or bone marrow from 12 patients with acute or chronic myeloid or myelomonocytic leukemia in whom karyotypic markers were present. The granulocytic colonies and clusters which developed on culture were shown to be derived from representative cells of the leukemic population. In two patients with acute leukemia in remission, normal colonies with a normal karyotype were grown from marrow cells but in two patients with chronic myeloid leukemia in remission the Ph1 abnormality persisted in colony cells. The agar culture technique appears to be ideal for following the emergence and disappearance of leukemic and normal granulopoietic populations in patients with these types of leukemia.
Immunodepression as a factor during 3‐methylcholanthrene carcinogenesis and subsequent tumour growth in mice
Authors: Rees Jennifer A. , Symes M. O. 
Published: February 2007

AbstractCBA/H‐T6 mice received a dose of 3 or 6 mg 3‐methylcholanthrene on day 0 and their spleen cell IgM antibody response to sheep red blood cells was found to be significantly depressed 23 days thereafter. However, at this time, there was no reduction in the ability of spleen cells from these mice to induce a graft‐versus‐host reaction in (A × T6)F1 hybrid animals. On day 68 after 3‐MC injection, six animals had developed tumours while six were still in the latent period. There was no difference in the capacity of spleen cells from these two groups of animals to induce a GvH reaction in F1 hybrids. Three 3‐MC induced sarcomata were serially passaged in isogenic hosts. The growth rates of these tumours increased and the regional lymph nodes and spleens became progressively more hypoplastic in successive hosts. However, there was again no decline in immunocompetence of spleen cells as judged by the induction of a GvH reaction in F1 hybrids. It is therefore suggested that the increase in tumour growth rate reflected a change in the tumour rather than increasing immunodepression of successive hosts.
Mechanism of resistance of yoshida sarcoma to nitrogen mustard. III. Mechanism of supressed transport of nitrogen mustard
Authors: Inaba Makoto 
Published: February 2007

AbstractMechanism of decreased transport of nitrogen mustard (HN2) in HN2‐resistant Yoshida sarcoma cells was investigated. HN2 uptake by Yoshida sarcoma cells proved to be temperature sensitive, saturable, affected by metabolic inhibitors, and also repressed competitively by choline chloride. In resistant cells, the uptake of choline was markedly depressed. Kinetics of choline transport in resistant cells was comparatively examined, and decreased Vmax and increased Km were observed in some resistant sublines. Accordingly, decrease in choline uptake may be due to decreased activity of choline transport‐carrier, both qualitatively and quantitatively. Decreased HN2 uptake by resistant cells seemed to be produced by the same mechanism.
Some international differences in histology and survival in breast cancer
Authors: Morrison Alan S. , Black Maurice M. , Lowe C. Ronald , Macmahon Brian , Yuasa Shu 
Published: February 2007

AbstractSurvival rates for patients with breast cancer have been reported to be higher in Japan than in the United States. It has also been reported that histologic features associated with more favorable survival are more frequent in Tokyo than in the United States. In this report data are presented on the question of whether differences in breast cancer histology could account for the differences in survival rate between Tokyo and two western areas–Boston, USA, and Glamorgan, Wales. Intraductal carcinoma was found to be most frequent in Tokyo and least frequent in Boston, but survival differences between the two cities were found when non‐invasive cases were excluded. Low (relatively malignant) nuclear grade was observed most often in Glamorgan; there was little difference in frequency of this characteristic between Boston and Tokyo. Since nuclear‐grade specific survival ratios were highest in Tokyo, this histologic feature also does not account for the better survival experience of Japanese patients. Tumors of medullary histologic type and those with high degrees of lymphoid infiltration were most frequent in Japan and least frequent in Boston. However, the relationships of histologic type and lymphoid infiltrate to survival were not consistent in the three areas, and the higher survival ratio of the Japanese patients could not be related to these characteristics.
Race and place in the etiology of nasopharyngeal cancer: A study based on California death certificates
Authors: Buell Philip 
Published: February 2007

AbstractA review of the California death certificates coded to nasopharyngeal cancer as cause of death during 1955–64 revealed that five of 273 white male decedents were born in the Philippine Islands or China, areas where the disease is known to be endemic. The expected number, based on certificates for other cancer deaths, was much less than one. From the death certificate entries for name and birthplace of parents of decedents it was apparent that the five decedents were of Caucasian stock without Mongoloid admixture. This is the first indication that there is a high risk of nasopharyngeal cancer not only for the Mongoloid peoples who are indigenous to Southeast Asia, but also for Whites born in the area.
Specific inhibition of sarcoma‐specific cellular immunity by sera from patients with growing sarcomas
Authors: Cohen Alfred M. , Ketcham Alfred S. , Morton Donald L. 
Published: February 2007

AbstractSarcoma‐specific cellular cytotoxicity was inhibited by sera from 14/16 patients with growing sarcomas, in comparison to only 1/8 patients with growing carcinomas and 4/14 sarcoma‐free patients. Of 11 completely inhibitory sarcoma sera, only two inhibited lung carcinoma‐specific cellular cytotoxicity. The presence of serum inhibition of sarcoma‐specific cellular cytotoxicity antedated the development of metastatic sarcoma.