bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of membrane‐associated proteins in human cholangiocarcinoma and hepatocellular carcinoma cell lines
Authors: Srisomsap Chantragan , Subhasitanont Pantipa , Sawangareetrakul Phannee , Chokchaichamnankit Daranee , Ngiwsara Lukana , Chiablaem Khajeelak , Svasti Jisnuson 
Published: December 2006

AbstractCholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC) occur with relatively high incidence in Thailand. Cell line models, originating from Thai patients, are available for both diseases, including the human bile duct epithelial carcinoma cell line (HuCCA‐1) and the HCC cell line HCC‐S102. Here, we have prepared subproteomes enriched in membrane proteins or in cytosolic proteins from the HuCCA‐1 and the HCC‐S102 cell lines. Study of differential protein expression by 2‐DE and LC/MS/MS showed 195 proteins expressed in the two cell lines, including both membrane‐associated and cytosolic proteins. Eighteen proteins were found in both membrane and cytosolic fractions of HuCCA‐1, but not in HCC‐S102, while nine proteins were found in both membrane and cytosolic fractions of HCC‐S102, but not in HuCCA‐1. Ten membrane proteins were found in HuCCA‐1 but not in HCC‐S102, including integrin alpha‐6 precursor, ezrin, hippocalcin‐like protein 1, mitogen‐activated protein kinase kinase kinase 2 (MAPK/ERK kinase kinase 2), and calgizzarin. Proteins showing increased expression in the membrane fraction of HuCCA‐1 were mainly cytoskeletal proteins (40.9%), while proteins showing increased expression in the membrane fraction of HCC‐S102 were mainly metabolic proteins (39.4%). The subproteomic approach used here facilitates detection of potential biomarkers undetected by regular proteomic methods.
A comparative proteomic study to characterize the vinblastine resistance in human ovarian cancer cells
Authors: Brown Dawn P. G. , Gökmen‐Polar Yesim , Jiang Li , Tan Jian , Ringham Heather , Janecki Dariusz J. , Qi Guihong , Witzmann Frank A. , Sledge George W. , Wang Mu 
Published: December 2006

AbstractDrug resistance is a major impediment to the successful treatment of human cancers, including ovarian cancer. Vinblastine (VLB), an antimicrotubule agent, is one of the chemotherapeutic drugs that exhibit resistance in ovarian cancer patients. To determine the protein factors that are involved in vinblastine resistance in human ovarian cancer cells, a combination of sample pre‐fractionation and high‐resolution 2‐DE proteomic analysis was performed. Approximately 1200 proteins were detected and quantitatively compared in both nuclear/membrane and cytosolic fractions. Sixty‐nine proteins from the nuclear/membrane fraction showed altered expression levels, whereas 59 were altered in the cytosolic fraction between SKOV3 (vinblastine‐sensitive) and SKVLB (vinblastine‐resistant) cell lines. These proteins include membrane‐associated, chromatin remodeling, cytoskeletal, and microtubule‐associated proteins as well as others that regulate signal transduction. This study not only demonstrates a novel understanding of the mechanism of drug resistance but also provides a valuable resource for future studies on drug resistance to vinblastine. In addition, it also represents a good example of how to increase the protein dynamic range and reduce sample complexity using currently available tools.
Resonant impurity states in PbTe‐based alloys doped with gallium and chromium under pressure
Authors: Skipetrov E. , Golubev A. , Pichugin N. , Plastun A. , Dmitriev N. , Slyn'ko V. 
Published: December 2006

AbstractThe galvanomagnetic effects in the n‐Pb1–x Snx Te:Ga (x = 0.21) and n‐Pb1–y Gey Te:Cr (y = 0.10) alloys at temperatures 4.2 ≤ T ≤ 300 K and under hydrostatic compression up to 17 kbar have been investigated. It is found that in almost the whole investigated pressure range in the samples investigated temperature dependencies of resistivity and Hall coefficient have a “metallic” character, indicating stabilization of the Fermi level by the impurity resonant levels. Using the experimental data in the framework of a two‐band Kane dispersion law the dependencies of the free electron concentration and the Fermi‐level position upon the pressure were calculated. The pressure coefficients of gallium and chromium deep‐level movement were obtained and the models of the electronic‐structure reconstruction under pressure were developed. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Raman scattering of capped and uncapped carbon‐induced Ge dots under hydrostatic pressure
Authors: Bernardi A. , Reparaz J. S. , Goñi A. R. , Alonso M. I. , Garriga M. 
Published: December 2006

AbstractWe compare results from Raman scattering for both capped and uncapped Ge quantum dots under hydrostatic pressure. The behavior of lattice vibrations and electronic transitions of the quantum dots are affected by the presence or absence of a cap layer. The pressure coefficient obtained for the Ge mode of relaxed, uncapped islands is close to the corresponding value in bulk, whereas it is reduced by ∼20% in presence of a Si cap layer. This result can be understood by taking into account the change of the boundary condition imposed to the Ge lattice, which is constrained to match that of the silicon matrix. Measurements of the capped sample exhibit a resonant enhancement which is not appreciable for the uncapped dots. We relate this effect to the different lifetime of electronic states. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Strength of strained quantum wells and other small scale structures
Authors: Dunstan D. J. , P'ng K. M. Y. , Zhu T. T. , Hou X. D. , Walker C. J. , Bushby A. J. 
Published: December 2006

AbstractThe strength of a material increases either when the structure is small or when only a small volume is under strain. The term ‘size effect’ covers generically all the ways in which this may happen. One manifestation of the size effect is in epitaxial growth of strained layers, for which critical thickness theory provides a satisfactory explanation. We have extended critical thickness theory to the bending and torsion of foils and wires of soft metals, and have built instruments for measuring the stress‐strain curves of soft metal foils with unprecedented accuracy to test this. Experimentally, semiconductor epitaxial growth provides structures with tailored internal strain distributions, ideal for helping to understand these problems. We have found that internal strains can reduce the strength of a superlattice by a factor of two at room temperature, but on the other hand can increase the strength by a factor of a hundred at high temperature. Nanoindentation on the semiconductor structures also reveals the size effect very clearly. All of these effects are clearly related to the finite volume required for the initiation of plasticity. New data is crucial to reconciling the various theoretical approaches to these problems. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
High pressure structure of Tb2Ti2O7 pyrochlore at cryogenic temperatures
Authors: Kumar Ravhi S. , Cornelius Andrew L. , Somayazulu Maddury , Errandonea Daniel , Nicol Malcolm F. , Gardner Jason 
Published: December 2006

AbstractThe structure of Tb2Ti2O7 pyrochlore was investigated at high pressures up to 24 GPa at cryogenic temperatures down to 6.5 K using angular dispersive X‐ray diffraction with synchrotron radiation at HPCAT, Advanced Photon Source. The cell parameters were obtained by performing full profile Rietveld refinements of the diffraction data. The equation of state is obtained at low temperatures by fitting the pressure‐volume data to a second order Birch Murnaghan eqation and a bulk modulus value of 168(4) GPa is obtained. The results show persistance of the pyrochlore structure up to the maximum pressure studied in the experiment and further indicate that pressure induces solely magnetic ordering which do not involve crystal symmetry changes. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
X‐ray diffraction study of amorphous phase of BaSi2 under high pressure
Authors: Nishii T. , Mizuno T. , Mori Y. , Takarabe K. , Imai M. , Kohara S. 
Published: December 2006

AbstractA high‐pressure synchrotron X‐ray diffraction study of zintl phase BaSi2 semiconductor has been performed up to 45 GPa. The pressure‐induced amorphization occurred at 13 GPa. In the amorphous phase, the Ba–Si bond distance decreased with increasing pressure, while the Ba–Ba bond distances were almost unchanged. The Rietveld refinement revealed that these distances in the crystal phase decreased with increasing pressure. By combining these results, the Ba–Si bond compressibility in both phases is almost identical. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Energetic and electronic properties of BN nanotube bundle under pressure
Authors: Guerini S. , Lemos V. , Piquini P. , Coutinho S. S. 
Published: December 2006

AbstractAb initio calculations were performed to study the structural transformation on boron nitride nanotubes bundles under pressure. The (16, 0) zigzag tube circular cross section was observed to suffer an oval deformation. The onset of this transformation was determined as 1 GPa, by observing discontinuous changes in the percent relative volume, and a center to center tube distance. The cohesive energy at the critical point was obtained. The band structure indicates the electronic properties to be preserved through the transition. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
New implementation of molecular double point‐group symmetry in four‐component relativistic Gaussian‐type spinors
Authors: Yanai Takeshi , Harrison Robert J. , Nakajima Takahito , Ishikawa Yasuyuki , Hirao Kimihiko 
Published: December 2006

AbstractA new, practical implementation of double‐group symmetry to relativistic Gaussian spinors is presented for four‐component relativistic molecular calculations. We show that the systematic adaptability to irreducible representations under arbitrary point‐group symmetry, as well as Kramers (time‐reversal) symmetry, is inherent in the present basis spinors, which possess the analytic structure of Dirac atomic spinors. The implementation of double‐group symmetry entails significant computational efficiencies in the relativistic second‐order Møller–Plesset perturbation calculation on Au2 and the density functional theory (DFT) calculation with the B3LYP functional on octahedral UF6, in which the highest symmetries used are, respectively, C and D. The four‐component B3LYP equilibrium geometry of UF6 is reported. © 2006 Wiley Periodicals, Inc. Int J Quantum Chem, 2007
Substituent effects on noncovalent halogen/π interactions: Theoretical study
Authors: Lu Yun‐Xiang , Zou Jian‐Wei , Wang Yan‐Hua , Yu Qing‐Sen 
Published: December 2006

AbstractNoncovalent halogen/π interactions of FCl with substituted benzenes have been investigated using ab initio calculations. It was shown that the predicted maximum interaction energy gap between the substituted and unsubstituted systems amounts to 1.14 kcal/mol, and therefore substituents on benzene have a pronounced effect on the strength of halogen/π interactions. While the presence of electron‐donating groups (NH2, CH3, and OH) on benzene enhances the interaction energy appreciably, an opposite effect is observed for electron‐accepting groups (NO2, CN, Br, Cl, and F). The large gain of the attraction by electron correlation illustrates that the stabilities of the systems considered arise primarily from the dispersion interaction. Beside the dispersion interaction, the charge‐transfer interaction also plays an important role in halogen/π interactions, as a charge density analysis suggested. To provide more insight into the nature of halogen/π interactions, topological analysis of the electron density distribution and properties of bond critical points were determined in terms of the atoms in molecules (AIM) theory. © 2006 Wiley Periodicals, Inc. Int J Quantum Chem, 2007
Torque teno virus (TTV): current status
Authors: Hino Shigeo , Miyata Hironori 
Published: December 2006

AbstractTorque teno virus (TTV), currently classified into the family Circoviridae, genus Anellovirus, was first found in a patient with non‐A‐E hepatitis. TTV has a single stranded circular DNA of ∼3.8 kb. TTVs are extraordinarily diverse, spanning five groups including SANBAN and SEN viruses. Torque teno mini virus (TTMV) with ∼2.9 kb genome also has wide variants. Recently, two related 2.2‐ and 2.6‐kb species joined this community. Recombinations between variants are frequent. This extensive TTV diversity remains unexplained; it is unclear how TTVs could be viable, and why they require such genetic variation. An unequivocal culture system is still not available. TTVs are ubiquitous in > 90% of adults worldwide but no human pathogenicity of TTV has been fully established. Epidemiological surveys need to specify the variants being studied and clinical targets, and must calibrate the sensitivity of the assay used. Potentially interesting observations include a higher viral load in patients with severe idiopathic inflammatory myopathies, cancer and lupus. Active replication was also found in infants with acute respiratory diseases. TTV/TTMV‐related viruses were found in chimpanzees, apes, African monkeys and tupaias, and also in chickens, pigs, cows, sheep and dogs. Experimentally, rhesus monkeys were persistently infected by TTV, but only 1/53 chimpanzees. TTV transcribes three species of mRNAs, 3.0‐, 1.2‐ and 1.0‐kb in the ratio of 60:5:35. Recently, at least three mRNAs were shown in chicken anaemia virus. The genomic region −154/−76 contains a critical promoter. TTV seems to have at least three proteins; however, the definite functions of these proteins await further research work. Copyright © 2006 John Wiley & Sons, Ltd.
High Electron Mobility InAs Nanowire Field‐Effect Transistors
Authors: Dayeh Shadi A. , Aplin David P. R. , Zhou Xiaotian , Yu Paul K. L. , Yu Edward T. , Wang Deli 
Published: December 2006

AbstractSingle‐crystal InAs nanowires (NWs) are synthesized using metal–organic chemical vapor deposition (MOCVD) and fabricated into NW field‐effect transistors (NWFETs) on a SiO2/n+‐Si substrate with a global n+‐Si back‐gate and sputtered SiOx/Au underlap top‐gate. For top‐gate NWFETs, we have developed a model that allows accurate estimation of characteristic NW parameters, including carrier field‐effect mobility and carrier concentration by taking into account series and leakage resistances, interface state capacitance, and top‐gate geometry. Both the back‐gate and the top‐gate NWFETs exhibit room‐temperature field‐effect mobility as high as 6580 cm2 V−1 s−1, which is the lower‐bound value without interface‐capacitance correction, and is the highest mobility reported to date in any semiconductor NW.
Pyrolysis of some acetoxynonenes
Authors: List G. R. , Evans C. D. , Selke E. , Glass C. A. , Hoffmann R. L. , McManis G. E. 
Published: December 2006

AbstractCurrent studies on odor thresholds of hydrocarbons autoxidatively derived from fats required the preparation of 1,3‐ and 2,4‐nonadienes. The pyrolysis of 3‐acetoxy‐1‐nonene (I) and 4‐acetoxy‐2‐nonene (II) was investigated as a route to 1,3‐ and 2,4‐nonadienes, respectively. The acetates were pyrolyzed over pyrex helices at 400 C. Distilled pyrolyzates were characterized by mass, UV, IR, and NMR spectroscopy, along with gas chromatography. Since II gave approximately a 50∶50 mixture of the 1,3‐ and 2,4‐isomers, an earlier observation was confirmed that allylic esters may rearrange before elimination. Ester I gave about 85% of the 1,3‐isomer and 15% of the 2,4. Presumably the 2,4‐isomer arises from thermal rearrangement. In pyrolysis, both I and II give conjugated products almost exclusively. UV spectroscopy shows ε values in the 24,000 to 28,000 range.
The turnover time of dietary cholesterol in the lactating rat
Authors: Easter D. J. 
Published: December 2006

AbstractThe secretion of dietary 4‐14C‐cholesterol into milk of the rat was determined as a function of post‐feeding time by a single dose technique. The time interval which elapsed before maximum specific radioactivity was reached in milk (17–20 hr after maximum activity in the serum) suggests a route through the mammary gland involving transport of the cholesterol by intracellular membranes. It also suggests that the exogenous cholesterol is incorporated into the milk fat globule membranes rather than into the fat globules during their synthesis within the cell.
Liver phospholipids of rats fed a choline‐deficient diet supplemented with choline or methionine
Authors: Beare‐Rogers Joyce L. 
Published: December 2006

AbstractThe low amount of arachidonic acid in the total phospholipids in the liver of rats fed a standard type of choline‐deficient diet was corrected by either choline or methionine, which also increased food intake. Choline increased the content of this fatty acid in the phosphatidyl ethanolamine but not in the phosphatidyl choline. Methionine increased both the amount of phosphatidyl choline and its content of arachidonic acid.
Lipids of bovine thyroid
Authors: Satyaswaroop P. G. 
Published: December 2006

AbstractThe lipid composition of the freshly slaughtered bovine thyroid tissue has been investigated. The phospholipid patterns of microsomal and mitochondrial fractions obtained from homogenates of bovine thyroids have also been determined. They resemble the phospholipid composition of the corresponding subcellular fractions from other tissues. The fatty acid composition of the various phospholipid species of these subcellular components have also been estimated by gas liquid chromatography. These analyses reveal that the fatty acids are not particularly characteristic of the subcellular organelle but tend to be characteristic of the lipid species. There is a high percentage of nervonic acid (C24∶1) in all the subcellular phospholipid species examined.
The triglyceride composition ofMoringa concanensis seed fat
Authors: Sengupta A. , Sengupta Chandana , Das P. K. 
Published: December 2006

AbstractMoringa concanensis seed fat and its randomized product have been subjected to pancreatic hydrolysis. Glyceride compositions have been calculated from the original fatty acid composition and those of the monoglycerides produced by hydrolysis. The per cent GS3 content of the interesterified product has also been determined by the combined techniques of thin layer chromatography on silver nitrate impregnated silica gel and colorimetry.
Fluorescent lipids of bovine brain white matter
Authors: Khan Afzal A. , Hess Helen H. 
Published: December 2006

AbstractTwo fluorescent lipids were isolated from a chloroform‐methanol (2∶1 v/v) extract of bovine brain white matter by two‐step preparative thin layer chromatography on Silica Gel G. The first step was performed with chloroform‐diethyl ether‐acetic acid (70∶30∶1 v/v) as developing solvent and revealed a single fluorescent band below the solvent front. The band was scraped off, and the lipid was eluted in chloroform and reapplied to Silica Gel G plates. In the second step, benzene‐methanol‐ethyl acetate (85∶10∶5 v/v) as developing solvent revealed two fluoresent bands (A and B) with Rf values of 0.72 and 0.65. The lipids were eluted and the UV and visible absorption spectra were measured in heptane, as were the excitation and fluorescence spectra. Sulfuric acid absorption spectra (2 and 24 hr treatment at 22 C) as well as the resulting excitation and fluorescence spectra were also determined. The fluorescent lipids reacted positively in a number of nonspecific color tests for steroids, but the chemical nature of these minor components of the neutral lipid fraction remains to be established.
Isovaleroyl triglycerides from the blubber and melon oils of the beluga whale (Delphinapterus leucas)
Authors: Litchfield Carter , Ackman R. G. , Sipos J. C. , Eaton C. A. 
Published: December 2006

AbstractThe fatty acid compositions of the blubber and melon oils from the beluga whale (Delphinapterus leucas) have been determined by gas liquid chromatography (GLC). The melon oil contains a high level (60.1 mole %) of isovaleric acid, substantial amounts of long chain branched acids (16.9%), and very little polyunsaturated material (0.5%). The blubber oil contains less isovaleric (13.2%), fewer long chain branched acids (2.7%), and appreciable amounts (10.9%) of the polyunsaturated acids typical of marine oils. The blubber and melon oils were also examined for lipid class composition by thin layer chromatography on silicic acid, direct GLC of the hydrogenated oil, and gel permeation chromatography. Both oils are composed almost entirely of triglycerides, which can be separated chromatographically into molecules containing 0, 1 and 2 isovaleric acid moieties. No triisovalerin could be detected. The blubber oil contains 68.9 mole % normal triacyl‐, 24.2% diacyl‐monoisovaleroyl‐, and 7.0% monoacyl‐diisovaleroyl‐triglycerides (acyl=long chain acid). Monoacyl‐diisovalerin constitutes 86.7 mole % of the melon oil. This unusual compound may play a role in the echolocation system of the beluga whale.
Sphingolipid biosynthesis by particulate fractions of normal and “Quaking” mouse brain
Authors: Kanfer Julian N. , Sargent Anne 
Published: December 2006

AbstractNo differences were detected in the ability of brain particulate fractions isolated from “Quaking” and normal mice for the incorporation of:14C‐L‐serine into 3‐keto‐dihydrosphingosine; 1‐14C‐stearoyl acid and 1‐14C‐stearoyl CoA into ceramide and14C‐choline from CDP14C‐choline into sphingomyelin.
Total fatty acids of habituated and teratoma tissue cultures of tobacco
Authors: Weete John D. 
Published: December 2006

AbstractSignificant differences in the total fatty acids of habituated and teratoma tissue cultures of tobacco were noted. The teratoma tissues contained 3.2 times more total fatty acids than the habituated, while both tissues differed considerably from the fatty acids of tobacco seedlings used for comparison. Differences in relative concentration for each of the three tissues were also noted.
Sterols in five coastal spermatophytes
Authors: Attaway D. H. , Haug P. , Parker P. L. 
Published: December 2006

AbstractSterolic fractions ofRuppia maritima L.,Diplanthera wrightii Aschers,Halophila engelmanni Aschers,Syringodium filiforme Kutzing andThalassia testudinum Konig have been isolated. The sterolic fractions were characterized by IR spectroscopy and tentative identities of their components were determined by gas chromatography and mass spectrometry.
Incorporation and metabolism of fatty acids by cultured dissociated cells from rat cerebrum
Authors: Yavin Ephraim , Menkes John H. 
Published: December 2006

AbstractDissociated brain cells in culture incorporate a variety of saturated and unsaturated fatty acids into their cellular lipids. Of the various fatty acids studied, uptake of radioactivity was greatest for stearic acid and decreased progressively with decreasing chain length. Incorporation of radioactivity from linoliec and linolenic acids was more extensive than from oleic acid. Cellular phospholipids and triacylglycerols were labeled preferentially from all fatty acid precursors, with the relative amount of label in phospholipids being greatest when cells were incubated with linolenic acid. Fatty acids underwent desaturation and chain elongation. Changes in the labeling pattern of phospholipid fatty acids in the course of incubation demonstrated precursor‐product relationships for laurate (12∶0), myristate (14∶0), palmitate (16∶0), and stearate (18∶0) and for linolenate (18∶3), eicosapentaenoate (20∶5), docosapentaenoate (22∶5), and docosahexaenoate (22∶6). The appearance of label in 22∶5 and 22∶6 paralleled the entrance of label into the ethanolamine phosphoglyceride fraction. Conversion of linoleate (18∶2 ω6) to arachidonate (20∶4 ω6) could be demonstrated but did not proceed via 18∶3 ω6.