bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silico bioprospecting of receptors associated to act mechanism of Rondonin, an antifungal peptide from spider Acanthoscurria rondoniae haemolymph
Authors: Seif Elias Jorge Muniz , Icimoto Marcelo Yudi , Junior Pedro Ismael Silva 
Published: March 2024

Abstract
Multiresistant fungal species are associated with the development of diseases. Thus, there is a need to develop more efficient drugs for the treatment of these etiological agents. Rondonin is a peptide isolated from the hemolymph of spiders Acanthoscurria rondoniae. Previous studies showed that this peptide presented antifungal activity against Candida sp. and Trichosporon sp. strains, acting in genetic material. However, the molecular targets involved in your biological activity have not yet been described. To determine possible targets involved in the biological activity of Rondonin using bioinformatics tools. The PharmMapper server was used to search microorganism targets for Rondonin. The PatchDock server was used to perform molecular docking. UCSF Chimera software was used to evaluate these intermolecular interactions. In addition, the I-TASSER server was used to predict the target ligand sites. In the end, all predictions were contrasted with the sites previously described in the literature. Rondonin was consistent with the ligand sites of the following targets: outer membrane proteins F (id:1MPF) and A (id:1QJP), responsible for allowing the passage of small molecules through the plasmatic membrane, subunit of the flavoprotein fumarate reductase (id:1D4E), responsible for the metabolism of nitrogenous bases, and ATP-dependent Holliday DNA helicase junction (id:1IN4), associated with histone proteins that fold the genetic material. These interactions corroborate previous in vitro studies on Rondonin, which acts on fungal genetic material without plasmatic membrane rupture. Therefore, the bioprospecting methods used in this research were considered satisfactory since they showed agreement with previous results obtained in vitro.
Bone marrow mesenchymal stem cell-derived exosome accelerate diabetic rat’s wound healing through inhibiting pyroptosis
Authors: Wu Yue , Wang Hongjin , Yu Miao , Liu Jun , Jin Yuanyuan , Cai Hui 
Published: March 2024

Abstract

Diabetic wounds are a type of wound that is characterized by protracted and refractory. In recent years, the use of mesenchymal stem cell-derived exosome(MSC-Exo)in wound treatment has made certain progress, especially bone marrow mesenchymal stem cell exosome (BMSC-Exo༉also have get achievement in wound treatment, and this paper aims to study whether bone marrow mesenchymal stem cell-derived exosomes can promote the healing of diabetic wound and its mechanism. We conducted in vivo and in vitro experiments to verify whether BMSC-Exo can promote wound healing and promote the proliferation and migration of fibroblasts after high glucose treatment in diabetic rats. The expression of NLRP3, IL-18, IL-1β, caspase-1,and GSDMD proteins in rats and cells in each group was detected to verify the relationship between the mechanism of BMSC-Exo promoting wound healing and cell pyroptosis. The results showed that BMSCs-Exo can significantly improve the wound healing rate in diabetic rats and promote the proliferation and migration of fibroblast under high glucose conditions. At the same time, we confirmed that the pyroptosis in diabetic rat wound and fibroblast in high sugar was significantly increased, and BMSCs-Exo could significantly inhibit the pyroptosis in diabetic rat wound and fibroblast. These results suggest that BMSC-Exo inhibits cell pyroptosis through NLRP3/ caspase-1/GSDMD pathway in vivo and in vitro, and promotes wound healing in diabetic rats.
Role of Supplementation with Selenium and Myo-inositol vs. Selenium alone in patients of Autoimmune Thyroiditis: A Systematic Review and Meta-Analysis
Authors: Zuhair Varisha , Shaikh Areeba Tufail , Shafi Nimra , Babar Areesha , Khan Areeb , Sadiq Arooba , Ashraf Muhammad Afnan , Nihan Khuld , Hamza Muhammad , Khalid Burhan , Fatima Syeda Haya , Ali Eman 
Published: March 2024

Abstract
Background
Autoimmune thyroiditis (AIT) is a condition characterized by inflammation of the thyroid gland. Hashimoto’s thyroiditis is a predominant type, involving the gradual destruction of the thyroid gland. It affects females more than males, with global prevalence of around 10–12%. Multiple studies imply that a combination of selenium and myo-inositol supplements can restore a euthyroid state in patients with auto-immune thyroiditis. The objective of this meta-analysis is to pool available evidence on effectiveness of supplementation therapy on treatment of AIT.
Methods:
Google scholar and PubMed databases were searched for randomized controlled trials (RCTs) and observational studies which reported outcomes of combined treatment in restoring a euthyroid state, specifically comparing it with selenium-only treatment. Changes in TSH, T3, T4, TPOAb, and TgAb levels from baseline were defined as indicators to compare the effect of combined vs. selenium only treatment in restoring euthyroid levels. The Cochrane risk of bias tool and Newcastle Ottawa Scale were used to assess the quality of the randomized control trials included in the study. For statistical analysis, Review Manager (version 5.4, Nordic Cochrane Centre, Copenhagen, Denmark) was used.
Result:
We pooled 3 studies enrolling 151 participants in MI + Se group and 137 participants in Se group. Supplementation of Se with MI demonstrated a significant reduction in TSH levels compared to Se alone (SMD= -1.15, 95% CI: -1.60 to -0.69, p < 0.00001). MI + Se treatment also significantly reduced TgAb levels compared to Se (SMD= -0.51, 95% CI: -0.78 to -0.24, p = 0.0002). In contrast, TPOAB, T3 and T4 levels were non-significantly reduced from baseline in patients treated with MI + Se when compared to Se alone (SMD= -0.81, 95% CI: -0.44 to 0.09, p = 0.20), (SMD = 0.16, 95% CI: -0.09 to 0.42, p = 0.22), and (SMD = 0.30, 95% CI: -0.23 to 0.83, p = 0.26) respectively.
Conclusion:
Supplementation of Se with MI showed a significant reduction in TSH and TgAb levels compared to selenium-only treatment, with non-significant reduction in TPOAB, T3 and T4 levels. This entails the need for powered clinical trials and observational studies with longer follow-ups to critically assess the role of combined therapy in restoring euthyroid state in patients with AIT.
Timestamp System for Causal Broadcast Communication
Authors: Muñoz-Fernández Isabel , Arévalo-Viñuales Sergio , Herás-Quirós Pedro De las 
Published: March 2024

Abstract

In unreliable asynchronous distributed systems with failures, achieving a causal view of the system across all processes is a challenging task. The Causal Reliable Broadcast (CRB) abstraction is used to solve this task. When CRB is implemented with algorithms that use logical vector clocks to timestamp broadcast events, the causal relationships between broadcast events can be detected with maximal accuracy. However, this timestamping mechanism used by CRB might not be useful for systems that need to reason about the causal relationships among both broadcast and delivery events. To address this challenge, the paper proposes a Causal Timestamp System (CTS) based on vector clocks that timestamps broadcast and delivery events capturing with maximal accuracy the causal relationships among those events. CTS simplifies the formal verification and testing of implementations of CRB algorithms based on CTS. Additionally, a new Global State Monitoring (GSM) algorithm is proposed, tailored to a distributed system that uses CRB with CTS. GSM enables finer-grained assessment of global states and application-dependent predicates of that system. We clarify these concepts with an IoT example.
LAYN is correlated with immune infiltration, cancer associated fibroblasts and prognosis in bladder cancer
Authors: Zhang Zitong , Liu Yang , Huang Xiaoxue , Li Yilin , Zhang Xingyue , Pan Qiwen , Cai Lingling , Lu Jiangli , Zhang Yijun , Gao Jianming , Liu Ruiqi , He Liru 
Published: March 2024

Abstract
Background
LAYN is a novel gene in liver cancer, non-small cell lung cancer and colorectal cancer for its diverse functions in tumor microenvironment (TME). However, the role of LAYN in shaping the TME remains to be elucidated in bladder cancer (BLCA).
Methods
Datasets (including RNA-sequencing, single-cell RNA-sequencing and survival data) were obtained from public databases. The correlation of LAYN with TME was analyzed with Tumor Immune Estimation Resource (TIMER) site. Immunohistochemistry from a single institution was used to verify the relationship between LAYN expression and TME marker levels and the efficacy of immunotherapy.
Results
Enrichment analysis revealed that LAYN overexpression was associated with the regulation of immune infiltration and cancer-associated fibroblasts (CAFs) pathways and functions in BLCA. Notably, LAYN overexpression led to increased immune infiltration but also an exhausted TME according to the correlation analysis of immune cells such as CD8 + T cells, CD4 + T cells, immune-related markers, and CAF-related markers in the TCGA database. These findings were validated by immunohistochemistry at a single institution. Furthermore, higher LAYN expression levels were associated with worse survival in the TCGA dataset, the IMvigor210 dataset and the cohort from our center. Additionally, LAYN, TIM-3, FOXP3 and FAP were linked to poor outcomes in patients treated with immunotherapy.
Conclusions
Our study revealed a correlation between high LAYN expression and abundant immune infiltration and CAFs in BLCA. However, LAYN may play a role in the regulation of immune suppression and escape and could serve as a potential predictive marker for immune checkpoint inhibitor response and patient survival in BLCA.
Photogenetics Inhibits the Activity of Glutamate Neurons Projected from the Motor Cortex to the Ventroposterolateral Nucleus of the Thalamus to Alleviates Central Post-Stroke Pain in Rats
Authors: Deng Chao , Gu Ya-juan , Xu Tao 
Published: March 2024

Abstract
The "central sensitization" and "central imbalance" theories suggest that central post-stroke pain (CPSP) is the result of neuronal overactivity and an imbalance between excitatory and inhibitory functions of the nervous system caused by damage to the sensory system. The ventral posterior lateral nucleus of the thalamus (VPL) and the primary motor cortex (M1) are key brain regions for nociceptive message transmission. However, the effects of inhibiting M1-VPL glutamatergic projections in CPSP remain unclear. his study investigates the efficacy of photogenetic inhibition of M1-VPL glutamatergic neuron excitability in a rat model of CPSP. CPSP rats underwent M1 injection with rAAV encoding an inhibitory photosensitive protein, followed by implantation of an optical fiber sleeve above the VPL and subsequent 10 W, 20 Hz, 5 ms yellow laser (589 nm) irradiation. The irradiation scheme was to start from the 3rd day after the CPSP mode, light for 2 min, turn off for 3 min, and repeat 6 times a day for 12 consecutive days. We observed that the mechanical and thermal pain thresholds were increased in CPSP rats, and the expressions of M1 pain-related factors c-Fos, vesicular glutamate transporter protein 2 (VGLUT2), and N-methyl-D-aspartate receptor subtype 2B (NR2B) were down-regulated. These findings indicate that photogenetic inhibition of M1-VPL glutamatergic projection can relieve pathologic pain in CPSP rats.
Morphological and molecular (SSRs) characterization of different diverse genotypes of blackgram (Vigna mungo L.)
Authors: Kumawat Radheshyam , Kumar Sanjeev , Kumar Susheel , Kashyap Subhash C. , Singh Aditi 
Published: March 2024

Abstract

Blackgram [Vigna mungo (L.) Hepper] is a leguminous crop and is an important source of plant-based protein. Thirty genotypes of Indian blackgram were analysed for genetic diversity using morphological, agronomical characters and SSR markers. Identifying elite lines is a major research priority for developing new varieties. The present investigation was carried out at the Advanced Centre for Rainfed Agriculture (ACRA) Dhiansar of SKUAST Jammu of UT of J & K during kharif 2021 to characterize all the eleven characters, viz., plant height, days to fifty percent flowering, number of branches per plant, days to maturity, number of pods per plant, number of seeds per pod, pod length, number of clusters per plant, 1000 seed weight and seed yield per plant morphologically and molecularly. Present study aimed to appraise highly diverse genotypes of blackgram and their associations between molecular markers and yield-contributing traits. Based on dendrograms and PCA values, results from field data and molecular data exhibited two genetically different groups of genotypes namely PU-15-2, PU-13-05 and AZAD-2 were found in group-1, while five genotypes, namely, PU-UPU-97-1, OU-40, PU-KUG216 and PU-15-32 were found in group-2 and such screened genotypes can be further used in the introgressions of specific traits from one group to another group of genotypes. Based on morphological and molecular data, two genetically diverse groups were constituted, and which can be recommended for further utilization in hybridization programs. Three markers namely viz., VR-102, CEDG-156 and CEDG-176 were identified and associated with seed yield per plant and could be useful in seed yield improvement programme of blackgram.
Análise do conhecimento dos professores de educação física da educação básica acerca do desenvolvimento psicomotor
Authors: Braz Letícia Maria Alvez , Silva Nadja Fonsêca da , Pontes Júnior José Airton de Freitas 
Published: March 2024

A psicomotricidade define-se como a relação dos sujeitos como movimento, levando em consideração os aspectos relacionais, afetivos e cognitivos. O desenvolvimento psicomotor contribui para evitar dificuldades de aprendizagem e reeduca o tônus, a postura, a direção, a lateralidade, o ritmo etc. O objetivo deste estudo foi avaliar o conhecimento dos professores de Educação Física da Educação Básica de Fortaleza-CE sobre o desenvolvimento psicomotor. Trata-se de uma metodologia descritiva e exploratória, de abordagem quali-quantitativa. A amostra contou com 16 professores de Educação Física de ambos sexos, com idades entre 24 e 51 anos. O instrumento utilizado para coleta de dados foi um questionário com questões sobre atuação e conhecimento voltados para a psicomotricidade. Os resultados demostraram que 43,8% dos participantes tiveram a disciplina de psicomotricidade durante a graduação e apenas 25% fizeram estágio na área, os demais não tiveram o conteúdo na graduação ou constou apenas superficialmente em outra disciplina. A maioria dos participantes utiliza ou busca utilizar a psicomotricidade em suas aulas e ressaltaram os desafios na utilização. O estudo destacou o contexto da Psicomotricidade nas aulas de Educação Física e fora do âmbito escolar, ressaltando a diferença de abordagem entre as duas e sua contribuição na vida dos indivíduos.
Palavras-chave: Psicomotricidade; Educação Física Escolar; Avaliação.
PROJETOS POLÍTICO-PEDAGÓGICO DE CURSOS DE PEDAGOGIA DO RS E AS CIÊNCIAS DA EDUCAÇÃO
Authors: Osório Mara Rejane Vieira 
Published: March 2024

Este trabalho problematiza a relação entre projetos político-pedagógicos (PPP) e as ciências da educação em cursos de pedagogia de universidades públicas do RS. O estudo foi de natureza qualitativa com análise documental e discursiva. Como inspiração teórica, a pesquisa seguiu pistas de estudos foucaultianos e sobre currículo. Argumento que o afastamento das ciências da educação, nos PPP, pode indicar um deslocamento das concepções humanistas, progressistas, para concepções utilitárias e pragmáticas.
Negative pressure protection with one-way surge tanks in bidirectional water delivery systems
Authors: Chang Yutong , Yu Xiaodong , Zhang Jian , Chen Xuyun , Chen Nan , Shi Lin 
Published: March 2024

ABSTRACT
Water hammer protection is one of the key factors for the safe operation of the long-distance water delivery systems, which has lots of useful results. However, the protection experiences for bidirectional water delivery systems using the same pipeline are lacking. In this study, the protection method with one-way surge tanks in the bidirectional water delivery system is investigated. The theoretical formula for one-way surge tank setting is derived based on the theory of water hammer propagation, and the pump head and bidirectional water hammer protection constraints. Subsequently, the pump trip transient processes in an actual system are simulated. Finally, different kinds of one-way surge tank protection schemes are compared. The results show that the pressure along the pipeline after the pump trip can meet the standard when using the one-way surge tank protection scheme calculated by the theoretical formula. The proposed method can provide the effective protection strategy for the bidirectional water delivery systems.
A holistic approach for performance evaluation of wastewater treatment plants: integrating grey water footprint and life cycle impact assessment
Authors: Jamshidi Shervin , Farsimadan Mohammad , Mohammadi Hanieh 
Published: March 2024

ABSTRACT

Wastewater treatment plants (WWTPs) have positive and negative impacts on the environment. Therefore, life cycle impact assessment (LCIA) can provide a more holistic framework for performance evaluation than the conventional approach. This study added water footprint (WF) to LCIA and defined ϕ index for accounting for the damage ratio of carbon footprint (CF) to WF. The application of these innovations was verified by comparing the performance of 26 WWTPs. These facilities are located in four different climates in Iran, serve between 1,900 and 980,000 people, and have treatment units like activated sludge, aerated lagoon, and stabilization pond. Here, grey water footprint (GWF) calculated the ecological impacts through typical pollutants. Blue water footprint (BWF) included the productive impacts of wastewater reuse, and CF estimated CO2 emissions from WWTPs. Results showed that GWF was the leading factor. ϕ was 4–7.5% and the average WF of WWTPs was 0.6 m3/ca, which reduced 84%, to 0.1 m³/ca, through wastewater reuse. Here, wastewater treatment and reuse in larger WWTPs, particularly with activated sludge had lower cumulative impacts. Since this method takes more items than the conventional approach, it is recommended for integrated evaluation of WWTPs, mainly in areas where the water–energy nexus is a paradigm for sustainable development.
Evaluation of sustainable development capacity of water sources: a case study of China
Authors: Zhang Jingqi , Jiang Shaohua 
Published: March 2024

Abstract
The issue of water scarcity has drawn attention from all over the world. The coordination of the interaction between ecological and environmental development of water sources and socio-economic development is currently an essential issue that needs to be solved in order to safeguard the water resources environment for human survival. In this essay, we suggest a paradigm for assessing the sustainable exploitation of water resources. First, three ecological, economic, and social factors are investigated. Twenty essential evaluation indexes are then constructed using the Delphi approach, along with an index system for assessing the potential of water sources for sustainable development. The weights of each evaluation index were then determined using the combination assignment approach, which was then suggested. The coupled degree evaluation model of the capability for sustainable development of water sources was then developed. In order to confirm the viability and validity of the suggested model, the model was used to assess the Liwu River water source's capacity for sustainable growth in the context of the South-North Water Transfer in Shandong, China. It is believed that the aforementioned study would serve as a helpful resource when evaluating the capacity of water sources for sustainable development.
Facile Synthesis of 2-Substituted Quinazolines via Ruthenium(II)-Catalyzed
Acceptorless Dehydrogenative Coupling
Authors: Wu Shulei , Lei Qianqian , Wu Zaoduan , Xie Huifang , Zhang Chen , Ye Yu , Yi Meiling , Chen Mingsheng , Xu Hao , Yang Zehua 
Published: March 2024

Abstract::
This study aims to develop a practical and facile one-pot synthesis of 2-
substituted quinazolines. Using a commercially available and structurally simple ruthenium(
II) complex as the catalyst to synthesize a series of quinazoline derivatives via acceptorless
dehydrogenative coupling. The mechanism of this reaction was explored by control
reaction and DFT calculation. This protocol offers access to a diverse array of quinazoline
derivatives (52 examples) in moderate to excellent yields (29%-98%). In summary, we
have developed an efficient one-pot ruthenium (II)-catalyzed ADC synthesis of quinazoline
under an air atmosphere. The reaction only produces hydrogen and water as by-products,
serving as a sustainable and atom-efficient synthetic approach.
Study on the Catecholase Activity in the Presence of a Mn(III)-based Catalyst and Base
Authors: Tripathy Rashmi Rekha , Sarkar Sohini , Jana Samaresh 
Published: March 2024

Background:
This work describes the oxidation of a catechol moiety to the corresponding qui-none by using Mn(III)-based catalysts in the presence of an organic base. Two newly reported Mn(III)-Schiff base complexes were utilized for the oxidation of three different substrates, namely, 3,5-di tert-butyl catechol, 1,4-dihydroxy benzene and pyrocatechol, aerobically.

Objective:
We intended to understand the role of a base for the above-mentioned oxidation process. Also, we were interested to monitor the progress of reaction using 1H-NMR spectroscopy and to get more insight into the mechanistic path of the reaction.

Methods:
The oxidation processes were studied in open air in acetonitrile or methanol solvent. The reaction mixture containing concerned substrate, catalyst, and base were stirred in open air and 1H-NMR spectrum was recorded using the crude reaction mixture in different time intervals.

Results:
Interestingly, introduction of a base in the reaction mixture, enhanced the rate to a great extent for the first two substrates. This observation may provide an idea toward the rate determining step of the pro-cess. Notably, the third substrate, pyrocatechol, could not be oxidized by any of the two catalysts even with a base. The oxidation of 1,4-dihydroxy benzene may emphasize monodentate binding mode of the sub-strate. Each of the oxidation was monitored with the help of time dependent 1H-NMR spectroscopy.

Conclusion:
A mechanistic pathway has been proposed. The spectra obtained in different cases help to compare the efficiency of the catalysts with or without base. In the absence of the catalysts, triethylamine alone cannot complete the conversion in the stipulated time, which may establish the effectiveness of the catalysts via the base-assisted mechanism.