bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANTI‐IgA ANTIBODIES OF LIMITED SPECIFICITY IN HEALTHY IgA DEFICIENT SUBJECTS
Authors: Koistinen J. , Cardenas R. M. , Fudenberg H. H. 
Published: April 2007

SUMMARYTo investigate the subclass and allotype specificity of anti‐IgA antibody synthesis, serum samples from 156 IgA deficient blood donors were screened for anti‐IgA antibodies by passive haemagglutination using IgA proteins of both subclasses (IgA1 and IgA2) as well as allotypes A2m(1) and A2m(2). Anti‐IgA activity was found in 25% (thirty‐nine). Antibodies were class‐specific in 19% (twenty‐nine) and of limited specificity in 6% (ten) of the samples. One unusual serum had anti‐IgA directed solely against IgA2. Its activity against A2m(2) was inhibited not only by A2m(2) protein but also by A2m(1) and by three IgA1 proteins. The anti‐A2m(1) activity of the same sample was inhibited only by A2m(1) proteins. The specific mechanism of IgA deficiency in this sample is discussed as well as the structural differences of the different allo‐ and subtypes of IgA and their relation to the antigenic properties of IgA. Inhibition studies could be performed with only one sample with anti‐IgA1 antibodies, but no allotypes of IgA1 were found. By haemagglutination inhibition, 15% (twenty‐three) of the samples contained minute amounts of IgA1, but no IgA2. None of the samples had only IgA2 or IgA1 and IgA2. Both findings, anti‐IgA antibodies with limited specificity in IgA deficient subjects and minute amounts of IgA of one subclass only in IgA deficient samples, are conceivable if IgA deficiency is caused by selective lack or defect of a subclass of B lymphocytes specifically synthesizing IgA2.
PURIFICATION AND PARTIAL CHARACTERIZATION OF THE apoA‐I OF RABBIT HIGH DENSITY LIPOPROTEIN
Authors: Børresen Anne‐Lise , Kindt T. J. 
Published: April 2007

SUMMARYPurfication of apoA‐I from rabbit high density lipoproteins (HDL) gave one single band in sodium dodecylsulphate‐polyacrylamide disc electrophoresis and reacted only with antiserum to apoA‐I. The molecular weight was about 25000. The amino acid composition of rabbit apoA‐I gave a difference index of 7.4 compared to human apoA‐I and of 6.5 compared to dog apoA‐I. Of the three carboxy terminal amino acid residues the rabbit protein has one in common with the dog.The amino acid sequence of twenty‐nine amino terminal residues showed 62% homology with the human protein; a minimum of thirteen base changes were required in the DNA sequences that encoded these two proteins. When the same sequence was compared with dog apoA‐I, it was found that ten base changes would be sufficient to account for the differences between the proteins.
THE cis AB PHENOTYPE IN THREE GENERATIONS OF ONE FAMILY: SEROLOGICAL, ENZYMATIC AND CYTOGENETIC STUDIES
Authors: Sabo Bernice H. , Bush Miriam , German J. , Carne L. R. , Yates A. D. , Watkins Winifred M. 
Published: April 2007

SUMMARYThe blood from three individuals belonging to consecutive generations of one family were characterized as cis AB on the basis of (a) the serological behaviour of the B antigen on their red cells, (b) the presence of weak anti‐B in their sera and (c) family studies, which unequivocally demonstrated the genotype AB: O for two of the individuals with atypical AB groups.The serological behaviour of the A antigen on the red cells was intermediated between that of normal A1 and A2 cell. The B antigen gave weak and variable reactions with naturally occurring antibodies. Treatment of the cis AB red cells with an α‐galactosyltransferase in the serum from an A1B individual rendered them agglutinable by the normal range of anti‐B sera, demonstrating that the red cells do not lack precursors for the formation of normal B‐active structures.The cis AB sera all contained relatively strong α‐N‐acetylgalactosaminyl‐transferases which had pH optima of 6.0, characteristic of A1 gene‐specified enzymes. The affinity of the enzymes for UDP‐galactose, as measured by the apparent Ki, appeared to be greater than that of the A transferases in normal A1 and A1B sera. The B‐gene transferases in the cis AB sera were very weak and were more readily inhibited by UDP‐N‐acetylgalactosamine than were the α‐galactosyltransferases in normal B and AB sera. Both the A‐ and B‐transferase activities in the cis AB sera are, therefore, in some ways different from the corresponding enzyme activities in the sera of normal A, B or AB donors.Cytogenetic study of blood lymphocytes from all three individuals showed normal karyotypes. Specifically, the terminal bands of the long arms of the No. 9 chromosomes, to which the ABO:Np‐1: AK‐1 linkage group has been assigned, displayed G bands similar to those in cells from other (normal) individuals examined simultaneously.
HLA ANTIGENS AS POSSIBLE MARKERS OF HETEROGENEITY IN SCHIZOPHRENIA
Authors: Iványi D. , Zemek P. , Iványi P. 
Published: April 2007

SUMMARYThe distribution of HLA‐A‐, ‐B‐ and ‐C‐locus antigens was tested in 200 male patients with final diagnosis of schizophrenia. A significant increase of HLA‐A28 and HLA‐Cw4 antigens and haplotype A10‐B18 was found. Indications were obtained for the increase of HLA‐Al in hebephrenic patients.It was presumed that the increase of Cw4 represents the common denominator of the diverse findings on paranoid schizophrenia. The increase of Cw4 indicates that the paranoid schizophrenia disease susceptibility locus is either the C locus itself or another closely linked locus (or loci). This would stress the importance of the HLA ‘central' regions for HLA and disease associations.A hypothesis is presented which points to the possibility that HLA antigens could be genetic markers of three ethiopathogenetic subgroups of schizophrenia. The possible tests of this hypothesis are also suggested.
α‐N‐ACETYL‐d‐GALACTOSAMIYL‐ AND α‐d‐GALACTOSYLTRANSFERASE ACTIVITIES IN SERA OF CIS AB BLOOD GROUP INDIVIDUALS
Authors: Badet J. , Ropar C. , Salmon C. 
Published: April 2007

SUMMARYThirteen cis AB persons from five families were examined for serum glycosyltransferase activities associated with the biosynthesis of A and B blood group characters. Their transferases were generally homogeneous within one family, except for A2/cis AB genotypes, whose A enzyme level was similar to the A2 normal sera, but they varied from one family to another. These activities differed quantitatively and qualitatively from A, B and AB normal sera. Studies of A transferase showed variations in the pH‐dependent curve, the effect of cofactors and the capacity of conversion of O red cells into A‐active cells. Moreover, A and B transferases behaved differently with respect to their relative levels than did AB heterozygous normal sera. The results were discussed and it was suggested that a mutation of a single enzyme transferring both galactose and N‐acetyl‐galactosamine could explain these properties.
EVIDENCE FOR A POSSIBLE MAJOR HISTOCOMPATIBILITY COMPLEX (BLA) IN CATTLE
Authors: Spooner R. L. , Leveziel H. , Grosclaude F. , Oliver R. A. , Vaiman M. 
Published: April 2007

SUMMARYLymphocytotoxic sera have been produced in forty‐seven cattle following skin grafting or lymphocyte immunization between dam‐offspring pairs. From these sera seventy antilymphocyte reagents have been produced. Seven of the sera were operationally monospecific without absorption, thirty‐seven others were made operationally monospecific and twenty‐six partially purified by absorption with lymphocytes. Thirty‐five of these sera were used in France and an additional thirty‐five were used in Scotland.The inheritance of the antigenic factors detected by these sera has been studied in 480 cattle families and the evidence is consistent with the hypothesis that at least seventeen of the factors are inherited in a simple Mendelian fashion. Fourteen of these factors show evidence of linkage with at least one other factor and are likely to be controlled by a limited number of closely linked loci. It is suggested that these loci should be identified as the BLA region.
GENETIC CONTROL OF ANTI‐DNP RESPONSE TO DNP‐BSA GIVEN BY CONTINUOUS INFUSION TECHNIQUE
Authors: Ikeda H. , Takizawa K. , Yamamoto T. , Odaka T. 
Published: April 2007

SummaryTen inbred strains of mice were administrated with dinitrophenyl‐bovine serum albumin (DNP‐BSA) at a dose of 30 or 300 μg/day by continuous infusion technique. Anti‐DNP plaque‐forming cells (PFC) in their spleens were assayed 10 to 12 days after the beginning of the infusion. NZB, BALB/c, C3H/He and NC strains were high responders to both doses of DNP‐BSA. KK, SII and TES strains were low responders: the antibody response was low to the dose of 30 μg/day, and high to the dose of 300 μg/day. Breeding tests between high responder BALB/c and intermediate responder DDD mice indicated that the immune response was largely controlled by a gene linked to the H‐2 complex. Similar studies with high responder NZB and low responder TES mice suggested an involvement of a few genes: at least one of the controlling genes may be linked to the H‐2 complex.
MOLECULAR PROPERTIES OF T‐LYMPHOMA IMMUNOGLOBULIN
Authors: Moseley Jane M. , Beatty Elizabeth A. , Marchalonis J. J. 
Published: April 2007

SUMMARYStructural studies of T‐cell immunoglobulin light chains have been carried out in order to ascertain whether they possess a unique structure or if they resemble standard kappa or lambda isotypes. T‐cell immunoglobulin was isolated from 125I‐labelled culture medium of monoclonal, continuously cultured T‐lymphoma cells, and the purified 125I‐labelled light chains were subjected to either cleavage by cyanogen bromide or digestion with trypsin. These peptides were then resolved and compared with those derived from 125I‐ and 131I‐labelled murine kappa and lambda chains in mixed label experiments, by means of polyacrylamide gel electrophoresis in sodium dodecyl sulfate‐containing buffers and ion exchange chromatography. The profiles obtained suggested that T‐lymphoma immunoglobulin light chains are immunoglobulin polypeptides and most closely resemble kappa chains. These results support available antigenic and mRNA hybridization data, which also suggest that T cells bear kappa‐like light chains.
HLA IN POPULATIONS: AN APPROACH FOR GENETICAL SUSCEPTIBILITY TO CANCER
Authors: Feingold Nicole , Degos L. , Feingold J. 
Published: April 2007

SUMMARYThe geographical correlations between the incidence of various cancers and the HLA and ABO antigen frequencies are studied. There is, for example, a positive correlation between breast and colorectal carcinoma and AI, B8 and B12 antigens, and a negative one between prostate carcinoma and B12.The role of the HLA system itself or other genes involved in these associations is discussed. This study gives some evidence of a possible genetic background of susceptibility or resistance to cancer.
GENETIC DIFFERENCES IN THE MITOGENIC RESPONSE OF PERIPHERAL BLOOD LYMPHOCYTES IN THE CHICKEN
Authors: Lassila O. , Nurmi T. , Eskola J. 
Published: April 2007

SUMMARYThe mitogenic responses of chicken peripheral blood lymphocytes were compared in two partially inbred chicken lines and in their hybrids. The lymphocytes of line P chickens showed a higher phytohaemagglutinin (PHA) response than those of line V chickens and (P x V)F1hybrids. The Con A responses were of the same magnitude in all chicken lines. The higher PHA response in line P chickens was not sex‐linked, apparently was not associated with the major histocompatibility complex and it did not correlate with immunoglobulin G allotypes.
DISCRIMINATION OF ANTIBODIES TO HEPATITIS B SURFACE ANTIGEN FROM ANTIBODIES TO INHERITED SERUM PROTEIN VARIANTS IN IMMUNE SERA OF HUMAN AND ANIMAL ORIGIN
Authors: Solaas Marit Hornberg 
Published: April 2007

SummaryAntisera to hepatitis B surface (HBs) antigen, both of human and rabbit origin, have been examined. The anti‐HBsserum derived from a multiply transfused patient did, in addition, contain antibodies directed against the inherited β‐lipoprotein antigen Ag(x), whereas one of the rabbit immune sera also contained antibody to the inherited Lp(a) antigen. Thus, in human, as well as in animal anti‐HBssera, antibodies to inherited normal serum antigens may cause false positive reactions. This problem may be overcome by absorption procedures if appropriate control systems are avialable. False positive reactions caused by the Ag(x) antigen may also be avoided by use of agarose as supporting medium for the test, as Ag(x) precipitin lines do not appear in this medium. An undialysable high molecular weight component may be obtained from Oxoid ‘Ionagar’ by washing. When this added to agarose the Ag(x) antigen reaction appears also in this medium.
DIFFERENCES IN THE SUSCEPTIBILITY OF MHC AND NON‐MHC MIXED LYMPHOCYTE REACTIONS TO SUPPRESSION BY MURINE AMNIONIC FLUID AND ITS COMPONENTS
Authors: Krco C. J. , Johnson Elizabeth , David Chella S. , Tomasi, Jr T. B. 
Published: April 2007

SummaryThe ability of mouse amniotic fluid (MAF), alpha‐foetoprotein (AFP) and MAF depleted of AFP (MAF ‐ AFP) to suppress primary one‐way MLR's was investigated. It was found that MAF, AFP and MAF ‐ AFP were all suppressive of MLR's specific for MHC, K, D or I + S determinants. Suppression was observed when either lymph node or spleen cells were used as the responder cells. Nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. In contrast, MLR's specific for non‐MHC/M‐locus determinants demonstrated either diminished suppression or augmentation of the response, compared with the MHC stimulated MLR's. Our results show a differential effect of whole MAF and its fractions on the proliferative responses induced by various allogeneic stimuli and suggest that suppression is not due to a non‐specific effect on proliferation regardless of the stimulus or cell type involved.
LITTER SIZES IN INBRED STRAINS OF RATS (RATTUS NORVEGICUS)
Authors: Gill T. J. , Kunz H. W. , Hansen C. T. 
Published: April 2007

SummaryThe average litter sizes and reproductive performance of twenty‐five inbred strains of rats, maintained in a conventional colony in Pittsburgh, and twenty‐six inbred strains, maintained in a barrier facility at the National Institutes of Health, were calculated from data collected for periods of 3 and 10 years, respectively. There were considerable variations in both parameters in each of the two colonies studied. In addition, there were quite wide variations in male to female ratios among inbred strains (0.80 to 1.42).
H‐2d‐LIKE SPECIFICITIES ON GARDNER (H‐2k) TUMOUR DETECTED IN A RESTRICTED ANTI‐TUMOUR REACTION
Authors: Matossian‐Rogers A. , Giorgi L. De , Festenstein H. 
Published: April 2007

SummaryCytostatis of the H‐2d tumour LSTRA by H‐2‐restricted effector lymphocytes was inhibited by antisera against H‐2.4 and H‐2.31 but not by antisera against public specificities or non‐H‐2 antigens. The unexpected reaction of the same effector cells against Gardner tumour (H‐2k) was also shown to be inhibited by a combination of antisera against H‐2.4 and H‐2.31 but not by each antiserum used separately. The inhibitory capacity of these antisera was removed by absorption with B10.D2 but not with B10 lymphocytes. This indicated the presence of H‐2d‐like specificities on gardner tumour which could function as self‐recognition structures in an H‐2Kd and H‐2Dd restricted system.
THE GENETIC REGULATION OF NK‐ACTIVITY: STUDIES ON CONGENIC MICE WITH B10 OR AKR BACKGROUND
Authors: Klein G. O. , Kärre K. , Klein G. , Kiessling R. 
Published: April 2007

SummaryMouse strains differ widely in their natural killer(NK)‐cell activity. In the (A X B6) X A backcross, high reactivity was linked to H‐2b, although non‐H‐2‐linked genes were also demonstrated (Petranyi et al., 1975). Harmon et al. (1977) demonstrated an H‐2Dd‐associated reactivity gene (1977). In the present study, we have tested eleven B10 congenic strains for NK activity. The H‐2Dd strains B10.A, B10.T(6R), B10.S(7R), B10.HTT and B10.D2 were more highly reactive than B10, B10.S, B10.G, B10.A(2R) and B10.BR, which do not carry the d allele at the H‐2D locus. While this confirms the H‐2Dd association of a reactivity gene, an exception was found in the B10.A(5R) strain that was low reactive in spite of the fact that it carries H‐2Dd. This suggests the possibility that the H‐2Dd‐associated gene is outside H‐2, to the right of Tla.The AKR.H‐2b congenic line had the same low activity as the AKR.H‐2k strain; both were much lower than B6. This suggests either one of two possibilities: the H‐2b‐linked reactivity gene is relatively distant from the H‐2 complex, although localized on chromosome 17 or alternatively, if localized within or in the close neighbourhood of H‐2, it requires non‐H‐2 genes for full expression.Previously, we have shown that the B6 X DBA/2 F1 hybrid was more highly reactive than either one of its two parental strains (Klein et al., 1978). A similar complementation effect is described in the present paper for the B10.D2 congenic strain. The high reactivity of this line can be due to the combined effect of an H‐2b‐linked gene from DBA/2 and the non‐H‐2 background of B10 or, alternatively, the former, together with an H‐2b‐linked gene from B6 that lies well outside outside the H‐2 locus itself.
HIGH FREQUENCY OF THE PROPERDIN FACTOR Bf F1 AND ITS LINKAGE TO HLA IN FRENCH BASQUES
Authors: Ohayon E. , Mouzon A. de , Hauptmann G. , Klein J. , Abbal M. , Constans J. , Mayer S. , Ducos J. 
Published: April 2007

SummaryWe studied 201 unrelated French Basque individuals for HLA and Bf polymorphisms. The haplotypes of eighty‐seven of them were deduced from family studies. The results show the frequency of theBf F1allele (0.1393) which is the highest one currently reported. They confirm the high frequencies of HLA‐Aw19.2 and B18 previously reported in that population and show that a whole haplotype with strong linkage disequilibria, namelyAw19.2, Cw5, B18, Bf F1, DRw3is frequent. On the other hand, the gene frequency ofBf Sis decreased (0.5497) as compared with the other European Caucasoïd populations, while a slight increase in theBf Fgene frequency (0.2960) appears. These results point out that it is of importance to consider the genetic background in choosing the population where linkage disequilibria are to be studied.
THE PUTATIVE ANTI‐THYROTROPIN RECEPTOR ANTIBODIES OF GRAVES' DISEASE:I. Gm ALLOTYPES *
Authors: Pepper Barbara , Noel Elke P. , Farid N. R. 
Published: April 2007

SummaryThe hyperthyroidism of Graves' disease is thought to be related to antithyrotropin receptor (TSH‐R) antibodies. In order to study the degree of immunogenetic homogeneity of these antibodies, we carried out Gm typing of ‘receptor‐purified’ IgG from patients with active Graves' disease and controls. The results were compared to those of serum, total IgG and IgG which failed to attach to TSH‐R. We found that in five out of seven Gm heterozygote patients studied the receptor‐purified antibodies were restricted to the products of one haplotype compared to three out of five similar controls. Such eluted antibodies were biologically active. Similar results in terms of immunogenetic restriction and activity were obtained when F(ab)2preparations were used. An unexpected finding was that sera and IgG from normal persons attached to thyroid membranes and that the attachment occurred via F(ab)2. Normal whole serum and ‘receptor‐purified’ IgG and F(2inhibited TSH binding in the receptor assay; however, this inhibition showed no specificity for TSH‐R.
HETEROLOGOUS TUMOUR GROWTH PATTERNS INDUCED IN RELATED MHC ‐ DEFINED CHICKEN LINES BY SEPARATE ISOLATES OF AN AVIAN SARCOMA VIRUS STRAIN
Authors: Cutting J. A. , Watanabe D. H. , Strebel F. R. , Vogt P. K. , McBride R. A. 
Published: April 2007

SummaryDifferent patterns of tumour growth resulted from inoculation of separate isolates of the Subgroup C Bratislava 77 strain (B77) of Avian Sarcoma Virus (ASV) into three closely‐related inbred lines of chickens. The major genetic difference between these chicken lines is that each is homozygous for a different MHC haplotype. Since for one of the viral isolates, resistance to progressive tumour growth has been shown to be controlled by MHC‐linked genes, the data presented here suggest that MHC‐controlled tumour rejection operates on viral or cellular determinants different from those which define classical viral group or subgroup specificity.
ERYTHROCYTE GLYCOSPHINGOLIPIDS OF FOUR SIBLINGS WITH THE RARE BLOOD GROUP p PHENOTYPE AND THEIR PARENTS
Authors: Kundu S. K. , Suzuki A. , Sabo Bernice , McCreary Joan , Niver E. , Harman R. , Marcus D. M. 
Published: April 2007

SUMMARYErythrocytes that exhibit the rare blood group p phenotype lack the P antigen (globotetraosylceramide) and the Pk antigen (globotriaosylceramide). This phenotype is inherited as an autosomal recessive condition and the red cells of heterozygous individuals, parents and children of p persons, are serologically normal but no chemical analyses of their red cells have been reported. We have studied an unusual family in which all five children exhibit the p phenotype. In addition to the abnormalities described previously, the erythrocytes of four siblings had twice the normal concentration of lactotriaoslyceramide and lactoneotetraosylceramide. These cells also contained 3‐5 times as much sialosyllactoneotetraosylceramide and up to a two‐fold increase in GM3 ganglioside. The glycolipids of the parents' erythrocytes were normal. Electrophoretic analysis of the glycoproteins of the proposita's erythrocytes revealed no abnormalities, but her erythrocyte membranes contained approximately 35% less galactosamine than normal red cells. This abnormality resulted from a marked decrease in galactosamine that was soluble in chloroformmethanol. The lipid‐extracted residue, which contained the glycoproteins, had a normal galactosamine content.
HAPLOTYPE STUDY OF THE HLA‐A, B, C AND Bf ASSOCIATIONS BY THE FACTORIAL CORRESPONDENCE ANALYSIS: A STUDY OF 325 HAPLOTYPES
Authors: Mayer Simone , Grange* D. , Tongio M. M. , Hauptmann G. , Klein J. 
Published: April 2007

SUMMARYThe factorial correspondence analysis, which is a descriptive statistical method, was used to study the preferential associations between locus HLA‐A, B, C and Bf alleles. This method does not limit the number ofloci that can be studied and must therefore be considered as an interesting method of studying haplotypic associations within the HLA system.
A NOVEL HYBRID SIALOGLYCOPROTEIN IN Sta POSITIVE HUMAN ERYTHROCYTES
Authors: Anstee D. J. , Mawby W. J. , Parsons S. F. , Tanner M. J. A. , Giles C. M. 
Published: April 2007

SummaryHuman erythrocytes expressing the rare blood group MNSs‐related antigen Sta (Stones) have an abnormal hybrid sialoglycoprotein designated (δ‐α)Sta. The abnormal (δ‐α)Sta sialoglycoprotein probably results from chromosomal misalignment with unequal crossing over between the α and δ genes in a manner analogous to that which gives rise to the anti‐Lepore‐type haemoglobins.
MONOCLONAL ANTI‐TYPE 2 H: AN ANTIBODY DETECTING A PRECURSOR OF THE A AND B BLOOD GROUP ANTIGENS
Authors: Knowles R. W. , Bai Y. , Daniels G. L. , Watkins Winifred 
Published: April 2007

SUMMARYA monoclonal antibody was produced which binds specifically to Type 2 H antigen of the ABO blood group system. The antibody, H11, is an IgM molecule which reacts by direct agglutination of red cells with the same pattern as other anti‐H reagents such as Ulex europaeus lectin. The specificity was determined by inhibition and adsorption with chemically defined oligosaccharides. H11 also reacted with poly(glycosyl)ceramide purified from group O red cells and glycoprotein H purified from human stomach mucosa and meconium. H11 differs from Ulex lectin, however, in that it was not inhibited by saliva from ABH secretor individuals or by glycoprotein H purified from human ovarian cysts or submaxillary glands.