bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brolucizumab effectiveness in pigment epithelium detachment due to exudative AMD in naïve treatment or non-responder patients
Authors: Furino Claudio , Albano Valeria , D’Addario Maria , Reibaldi Michele , Boscia Francesco , Alessio Giovanni 
Published: March 2024

Purpose To investigate outcomes after Brolucizumab injection in naïve treatment or non-responder patients with exudative age-related macular degeneration (AMD). Methods It is a retrospective, comparative, cohort study conducted at the tertiary referral center of the University Hospital Polyclinic of Bari, for 5 years, from November 2017 until May 2022. 41 eyes with wet-AMD (w-AMD) were included, undergoing anti-vascular endothelial growth factor (VEGF) intravitreal injections. The sample was divided into two groups, the Bro-Switch group, and the Bro-Naïve group. The Bro-Switch group previously received a slot of other anti-VEGF intravitreal drugs. The Bro-Naïve group received Brolucizumab (Bro) as the first treatment. The pigment epithelium detachment (PED) and the best-corrected visual acuity (BCVA) outcomes before and after Bro-injection were evaluated. Results A significant reduction in PED measurement was registered in all eyes treated with Bro-injection ( p = 0.35). The Bro-Naïve group improved better in PED measurement (mean difference: 297.92 ± 72,32) as compared to the Bro-Switch group (mean difference: 185.06 ± 11.07). On the contrary, no significant reduction in BCVA in the two groups was recorded ( p = 0.66). Conclusion We suggest Bro-injection for w-AMD as effective anatomical outcomes in PED flattening, but not similar in visual results. Although this study evaluated short-term outcomes, the hopeful results can lead to interesting medium-long time effects.
Plasticizing Pregnancy: Microplastics Identified in Expectant Mothers’ Feces
Authors: Hasanah Uswatun , Amqam Hasnawati , Septami AR Ervina , Chalid Maisuri , Aris Ahmad Zaharin 
Published: March 2024

Introduction: Microplastics may be present in food and drinks from various sources, exposing pregnant women to these particles. Consumption of contaminated food can lead to the ingestion of microplastics by pregnant women, potentially causing adverse health effects on the fetus. This study aims to investigate the presence of microplastics in the stools of pregnant women. Methods: The research was conducted in the Makassar City region of South Sulawesi, Indonesia. Thirty healthy pregnant women from 2 community health centers, Pattingalloang and Jumpandang Baru, participated in the study. Their stools were analyzed using Fourier Transform Infrared (FTIR) microspectroscopy to detect the presence of microplastics. Result: The analysis revealed the presence of a total of 359 microplastics in the participants’ stools, with particle counts ranging from 4 to 21 and sizes ranging from 0.2 to 4.9 mm per 25 g of stool. The polymers identified included Polyethylene Terephthalate (PET), Polyamide/Nylon, Polyethylene Chlorinated, HDPE, and Ethylene Propylene. The amount of microplastics varied significantly among groups with different levels of seafood consumption. Conclusion: Indonesian pregnant women have been exposed to some microplastic polymers.
How healthcare systems are experienced by autistic adults in the United Kingdom: A meta-ethnography
Authors: Radev Sarah , Freeth Megan , Thompson Andrew R 
Published: March 2024

Autistic adults are at increased risk of both mental and physical health difficulties, and yet can face barriers to accessing healthcare. A meta-ethnographic approach was used to conduct a review of the existing literature regarding autistic adults’ experiences of accessing healthcare. Four databases were systematically searched for qualitative and mixed-method studies reporting on the experiences of autistic adults without a co-occurring learning disability accessing adult healthcare services within the United Kingdom. Fifteen studies met the inclusion criteria, and seven steps were used to systematically extract the data and then generate novel themes. Three superordinate themes were identified: Professionals’ lack of knowledge can be damaging, Need to reduce processing demands and Adaptation to improve engagement. This review highlights the wide-reaching damaging impact misdiagnosis, inadequate or inappropriate treatment, overwhelming environments and inaccessible systems can have on the well-being and ability of autistic adults to engage with treatment. The lack of autism knowledge and understanding experienced in interactions with healthcare professionals, along with autistic adult’s own communication and sensory processing differences, demonstrates the need for widely delivered training co-produced with autistic adults alongside bespoke and person-centred adaptations. Lay abstract Autistic adults are more likely to experience mental and physical health difficulties, and yet can find it difficult to get the support that they need. A meta-ethnographic approach was used to review the existing research on autistic adults’ experiences of accessing healthcare. Four databases were searched for qualitative and mixed-method studies which looked at the experiences of autistic adults who did not also have a learning disability when using healthcare services in the United Kingdom. Fifteen papers met the criteria to be included, and seven steps were used to analyse the information and develop new themes. Three main themes were identified: Professionals’ lack of knowledge can be damaging, Need to reduce processing demands and Adaptation to improve engagement. This review highlights how damaging misdiagnosis, inappropriate treatment, overwhelming environments and systems that are difficult to access can have on the well-being of autistic adults. Limited knowledge and understanding about autism knowledge among healthcare professionals along with autistic adult’s own communication and sensory differences indicate that there is a need for improved training developed with autistic adults and adaptations.
The Perceval Sutureless Bioprosthetic Aortic Valve: Evolution of Surgical Valve Technology
Authors: Fialka Nicholas M. , EL-Andari Ryaan , Wang Shaohua , Dokollari Aleksander , Kent William D. T. , Fatehi Hassanabad Ali 
Published: March 2024

Objective: The surgical treatment of aortic stenosis continues to evolve, and sutureless aortic valve replacement (SUAVR) is an emerging technology. With the Perceval S (Corcym, London, UK) as the only true sutureless valve on the market, the objective of this review is to analyze the current literature on Perceval S. Focusing on valve design and deployment as well as applications of the technology for challenging pathology, clinical outcomes are assessed, including a comparison with transcatheter AVR (TAVR). Methods: PubMed and MEDLINE were searched by 3 authors for studies analyzing SUAVR from inception to May 19, 2023. Results: SUAVR facilitates minimally invasive surgery and offers an alternative strategy for patients with small aortic annuli. It also has a time-saving advantage for patients who require complex operations. SUAVR results in excellent long-term morbidity, mortality, durability, and hemodynamic function. In comparison with conventional surgical AVR (SAVR), SUAVR does have a greater risk of postoperative pacemaker implantation; however, increasing user experience and refinements in implantation technique have contributed to reductions in this outcome. SUAVR results in morbidity and mortality that is similar to rapid-deployment AVR. Midterm outcomes are superior to TAVR; however, further robust investigation into all of these comparisons is ultimately necessary. Conclusions: SUAVR bridges the gap in technology between SAVR and TAVR. The application of this exciting technology will undoubtedly grow in the coming years, during which additional investigation is paramount to optimize preoperative planning, valve deployment, and reintervention strategies.
Cine clips increase the detection of thyroid pyramidal lobe in routine thyroid sonogram
Authors: Yan Yi , He Xiaoou , Hu Kai , Frankel Jed , Liu Jian , Goubran Ashraf , Karlicki Fern 
Published: March 2024

Background: Identification and resection of the thyroid pyramidal lobe is important for thyroid cancer surgery in order to prevent interval cancer in residual thyroid tissue. Purpose: The purpose of this study was to determine how often a thyroid pyramidal lobe is found in patients with and without previous thyroidectomy and to optimise the protocol for identifying thyroid pyramidal lobes during routine thyroid ultrasonography. Material and Methods: In this prospective study, a total of 1579 patients who received routine thyroid ultrasound scans at a single centre were enrolled. A dedicated standard scanning protocol was established containing both static images of the anterior neck superior to the thyroid as well as a transverse cine loop starting from the isthmus to the hyoid bone. The presence and features of thyroid pyramidal lobes were evaluated and compared. Results: Detection rate of thyroid pyramidal lobes in patients without thyroidectomy improved from 39.5% (480/1215) to 49.7% (640/1215) with protocol adding cine-loop as compared to protocol without cine-loop. The cine-loops were particularly helpful in the detection of thyroid pyramidal lobes when it is separated from the main lobe or in thyroidectomy patients. By adding assessment with cine-loop into the dedicated protocol, we have further detected different pathologies occurring on thyroid pyramidal lobes including benign and malignant solid nodules and pseudo-nodules of Hashimoto’s thyroiditis. Conclusion: The addition of dynamic assessment with cine-loop increases the detection rate of thyroid pyramidal lobes. By paying attention to the thyroid pyramidal lobe in pre-operative diagnostic sonographic images, we can help to avoid incomplete removal of the thyroid gland during thyroidectomy.
Safe usage of Mini ‘C’-arm in operating room environment: Implications for clinical practice
Authors: Naidu Jatin , Botchu Rajesh , Iyengar Karthikeyan P 
Published: March 2024

Background: Mini ‘C’-arm machine is an advanced medical imaging device used primarily for intraoperative imaging during surgical, orthopaedic and emergency care procedures. Since the technology is based on ionising radiation, safe usage of Mini ‘C’-arm machine is mandatory to protect patients and operating personnel. Objective: The main objective is to describe the various components related to patients, operator and equipment to ensure safe usage of Mini ‘C’-arm machine. A comprehensive search strategy using the PEO (Population, Exposure, Outcome) framework was conducted using Embase, PubMed, Google Scholar and ResearchGate databases to identify suitable literature. The keywords used for the search included ‘Fluoroscopy’, ‘Ionising Radiation’ and ‘surgical safety’. Key findings: Safe usage of Mini ‘C’-arm equipment involves components of operator training, operator safety, patient safety, radiation dose, operating room logistics, handling of images and auditing of Mini ‘C’-arm use. Conclusion: Mini ‘C’-arm provides an invaluable, portable imaging tool in a spectrum of general surgical and orthopaedic interventional procedures. However, safe usage of Mini ‘C’-arm machine requires a multifaceted approach including operator responsibility and safety, patient protection, equipment maintenance, radiation dose awareness, documentation and sound reporting mechanisms.
Royal Jelly, A Super Food, Protects Against Celecoxib-Induced Renal Toxicity in Adult Male Albino Rats
Authors: Khalifa Hesham A. M. I. , Eleiwa Naglaa Z. H. , Nazim Heba A. 
Published: March 2024

Background: Celecoxib is a COX-2 nonsteroidal anti-inflammatory drug (NSAID). It is widely used for the treatment of osteoarthritis, rheumatoid arthritis, and ankylosing spondylitis. Objective: This study aimed to explore the effect of long-term administration of celecoxib on kidney of male albino rats, and to study the potential effect of treatment discontinuation on such tissues. The study also examined the alleged ameliorative effect of royal jelly (RJ). Methods: Fifty, male albino rats were divided into 5 equal groups; 10 each. Group 1: rats received no drug (control group). Group 2: rats received celecoxib (50 mg/kg/day, orally for 30 successive days). Group 3: rats received celecoxib (50 mg/kg/day, orally) and royal jelly (300 mg/kg/day, orally) for 30 successive days. Group 4: rats received celecoxib for 30 successive days, then rats were left untreated for another 30 days. Group 5: rats received celecoxib and RJ for 30 successive days, then rats were left untreated for another 30 days. Results: Long-term celecoxib administration caused significant elevation in kidney function tests, with ameliorative effects of RJ against celecoxib-induced renal toxicity. Conclusion: Long-term celecoxib administration caused renal toxicity in male albino rats, with ameliorative effects of RJ.
Perceived Stress and Level of Uncertainty Among Hospitalized COVID-19 Patients
Authors: Nabi Foodani Mahdi , Abbasi Dolatabadi Zahra , Rahbariyan Amir , Rasti Arezoo , Jafaryparvar Zakiyeh , Zakerimoghadam Masoumeh 
Published: March 2024

Introduction Disease uncertainty refers to the inability to assign meaning to events related to the illness. Uncertainty of the disease can affect various aspects of human life such as psychological aspects. Objectives This study aims to examine the relationship between disease uncertainty and perceived stress in COVID-19 patients. Methods An analytical cross-sectional study was conducted on 212 hospitalized COVID-19 patients who were initially admitted to the intensive care units (ICUs) and later transferred to general wards within the same hospitals. Three instruments were utilized to collect data for this study. The Demographic Information Questionnaire, Mishel Uncertainty in Illness Scale (MUIS) for disease uncertainty, and Perceived Stress Questionnaire. For data analysis, both descriptive and inferential statistics were employed using IBM SPSS Statistics version 25. Results The Pearson correlation coefficient matrix results showed a positive and significant relationship between uncertainty about the illness ( P < .001, r = 0.829), ambiguity ( P < .001, r = 0.795), complexity ( P < .001, r = 0.835), inconsistency or instability ( P < .001, r = 0.787), and unpredictability ( P < .001, r = 0.776) with perceived stress in COVID-19 patients transferred from the intensive care units. Conclusion Based on the findings of the current study, both uncertainty and perceived stress are elevated among COVID-19 patients, and a significant and direct relationship exists between these two variables. Healthcare providers, particularly nurses, should address the uncertainties surrounding emerging diseases, both at the hospital and community levels.
A cascading learning method with SegFormer for radiographic measurement of periodontal bone loss
Authors: Yu Hanwen , Ye Xin , Hong Wanjing , Shi Rui , Ding Yi , Liu Chengcheng 
Published: March 2024

Abstract
Objective
Marginal alveolar bone loss is one of the key features of periodontitis and can be observed via panoramic radiographs. This study aimed to establish a cascading learning method with deep learning (DL) for precise radiographic bone loss (RBL) measurements at specific tooth positions.

Materials and methods
Through the design of two tasks for tooth position recognition and tooth semantic segmentation using the SegFormer model, specific tooth’s crown, intrabony portion, and suprabony portion of the roots were obtained. The RBL was subsequently measured by length through these three areas using the principal component analysis (PCA) principal axis.

Results
The average intersection over union (IoU) for the tooth position recognition task was 0.8906, with an F1-score of 0.9338. The average IoU for the tooth semantic segmentation task was 0.8465, with an F1-score of 0.9138. When the two tasks were combined, the average IoU was 0.7889, with an F1-score of 0.8674. The correlation coefficient between the RBL prediction results based on the PCA principal axis and the clinicians’ measurements exceeded 0.85. Compared to those of the other two methods, the average precision of the predicted RBL was 0.7722, the average sensitivity was 0.7416, and the average F1-score was 0.7444.

Conclusions
The method for predicting RBL using DL and PCA produced promising results, offering rapid and reliable auxiliary information for future periodontal disease diagnosis.

Clinical relevance
Precise RBL measurements are important for periodontal diagnosis. The proposed RBL-SF can measure RBL at specific tooth positions and assign the bone loss stage. The ability of the RBL-SF to measure RBL at specific tooth positions can guide clinicians to a certain extent in the accurate diagnosis of periodontitis.
Interprofessional collaboration skills and motivation one year after an interprofessional educational intervention for undergraduate medical and nursing students
Authors: Teuwen Carolyn Joyce , Kusurkar Rashmi A. , Schreurs Hermien , Daelmans Hester E. M. , Peerdeman Saskia M. 
Published: March 2024

Abstract
Background
The increasingly complex patient care in the twenty-first century is delivered by interprofessional health care teams. Interprofessional collaboration can be taught during interprofessional education. However, whether a long-term change in collaborative competencies can be achieved by interprofessional education has not been studied sufficiently. Our research questions were: How does motivation for interprofessional collaboration and interprofessional collaborative skills change up to one year after an interprofessional educational intervention? How are they related to each other?

Methods
During a one-year period, undergraduate medical and nursing students attended four interprofessional (intervention) or uniprofessional (control group) education sessions. Self-determination Theory was used as the theoretical framework. Autonomous and controlled motivation scores for interprofessional collaboration were calculated using the Academic Self-Regulation Questionnaire, before (T1), directly after (T2) and one year post-intervention (T3). At T3, the students also filled out the Interprofessional Collaborative Competencies Attainment Survey (ICCAS), which measured the perceived attainment of collaborative competencies by a retrospective pre-test/post-test design. We used linear mixed effects models to analyse the motivation scores and linear regression for the relation between motivation and competence.

Results
In the interprofessional group, autonomous motivation scores of the participants were significantly lower at T2 vs. T1. Controlled motivation scores were significantly higher at T3 vs. T1. Controlled motivation scores for T2 were significantly higher in the uniprofessional group than in the interprofessional group. Perceived competence was related to higher autonomous motivation scores. At T3 the interprofessional collaborative competencies seemed to have grown more among students in the interprofessional group.

Conclusions
The perceived growth in interprofessional collaboration competence lasted at least up to one year after the intervention, and was measurable with the ICCAS. The growth was significantly more in the IPE students than in the UPE students. The few differences found in motivation scores for interprofessional collaboration were probably caused by an imbalance of nursing versus medical students over the different time points. This finding indicates that classroom based IPE can contribute to interprofessional collaboration skills of nursing and medical students at least up to one year after an intervention.
Nanoscale myelinogenesis image in developing brain via super-resolution nanoscopy by near-infrared emissive curcumin-BODIPY derivatives
Authors: Chen Junyang , Yu Yifan , Wang Siyou , Shen Yu , Tian Yupeng , Rizzello Loris , Luo Kui , Tian Xiaohe , Wang Tinghua , Xiong Liulin 
Published: March 2024

AbstractUnderstanding the intricate nanoscale architecture of neuronal myelin during central nervous system development is of utmost importance. However, current visualization methods heavily rely on electron microscopy or indirect fluorescent method, lacking direct and real-time imaging capabilities. Here, we introduce a breakthrough near-infrared emissive curcumin-BODIPY derivative (MyL-1) that enables direct visualization of myelin structure in brain tissues. The remarkable compatibility of MyL-1 with stimulated emission depletion nanoscopy allows for unprecedented super-resolution imaging of myelin ultrastructure. Through this innovative approach, we comprehensively characterize the nanoscale myelinogenesis in three dimensions over the course of brain development, spanning from infancy to adulthood in mouse models. Moreover, we investigate the correlation between myelin substances and Myelin Basic Protein (MBP), shedding light on the essential role of MBP in facilitating myelinogenesis during vertebral development. This novel material, MyL-1, opens up new avenues for studying and understanding the intricate process of myelinogenesis in a direct and non-invasive manner, paving the way for further advancements in the field of nanoscale neuroimaging.
Graphical Abstract
Development of a time-resolved fluorescence immunoassay kit for detecting canine coronavirus and parvovirus through double labeling
Authors: Li Laiqing , Chen Cuicui , Liang Huankun , Dong Wenqi , Leontiev V. N. , Voytov Igor Vitalievich 
Published: March 2024

Abstract
Objective
Canine enteric coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the main pathogens responsible for acute gastroenteritis in dogs, and both single and mixed infections are common. This study aimed to establish a double-labeling time-resolved fluorescence immunoassay (TRFIA) to test and distinguish CCV and CPV-2 diseases.

Methods
A sandwich double-labeling TRFIA method was established and optimized using europium(III) (Eu3+)/samarium(III) (Sm3+) chelates. CCV/CPV-2 antigens were first captured by the immobilized antibodies. Then, combined with Eu3+/Sm3+-labeled paired antibodies, the Eu3+/Sm3+ fluorescence values were detected after dissociation to calculate the CCV/CPV-2 ratios. The performance, clinical performance and methodology used for laboratory (sensitivity, specificity, accuracy and stability) testing were evaluated.

Results
A double-label TRFIA for CCV and CPV-2 detection was optimized and established. The sensitivity of this TRFIA kit was 0.51 ng/mL for CCV and 0.80 ng/mL for CPV-2, with high specificity for CCV and CPV-2. All the accuracy data were less than 10%, and the recovery ranged from 101.21 to 110.28%. The kits can be temporarily stored for 20 days at 4 °C and can be stored for 12 months at temperatures less than − 20 °C. Based on a methodology comparison of 137 clinically suspected patients, there was no statistically significant difference between the TRFIA kit and the PCR method. Additionally, for CCV detection, the clinical sensitivity was 95.74%, and the clinical specificity was 93.33%. For CPV-2 detection, the clinical sensitivity was 92.86%, and the clinical specificity was 96.97%.

Conclusion
In this study, a double-label TRFIA kit was prepared for CCV and CPV-2 detection with high laboratory sensitivity, specificity, accuracy, stability, clinical sensitivity and specificity. This kit provides a new option for screening/distinguishing between CCV and CPV-2 and may help improve strategies to prevent and control animal infectious diseases in the future.