bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a multiplex polymerase chain reaction assay for detection of hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1
Authors: Nemr Waleed Abdelgaber , Nashwa Radwan K 
Published: March 2024

BACKGROUND
Hepatitis C virus (HCV), hepatitis B virus (HBV), and human immunodeficiency virus 1 (HIV-1) are the most epidemic blood-borne viruses, posing threats to human health and causing economic losses to nations for combating the infection transmission. The diagnostic methodologies that depend on the detection of viral nucleic acids are much more expensive, but they are more accurate than serological testing.
AIM
To develop a rapid, cost-effective, and accurate diagnostic multiplex polymerase chain reaction (PCR) assay for simultaneous detection of HCV, HBV, and HIV-1.
METHODS
The design of the proposed PCR assay targets the amplification of a short conserved region featured with a distinguishable melting profile and electrophoretic molecular weight inside each viral genome. Therefore, this diagnostic method will be appropriate for application in both conventional (combined with electrophoresis) and real-time PCR facilities. Confirmatory in silico investigations were conducted to prove the capability of the approached PCR assay to detect variants of each virus. Then, Egyptian isolates of each virus were subjected to the wet lab examination using the given diagnostic assay.
RESULTS
The in silico investigations confirmed that the PCR primers can match many viral variants in a multiplex PCR assay. The wet lab experiment proved the efficiency of the assay in distinguishing each viral type through high-resolution melting analysis. Compared to related published assays, the proposed assay in the current study is more sensitive and competitive with many expensive PCR assays.
CONCLUSION
This study provides a simple, cost-effective, and sensitive diagnostic PCR assay facilitating the detection of the most epidemic blood-borne viruses; this makes the proposed assay promising to be substitutive for the mistakable and cheap serological-based assays.
Growth of Cut-Grafting Robusta Coffee Seeds Utilizing Orthotropic and Plagiotropic Rootstocks with Application Bacillus and Pseudomonas Mixture
Authors: Rosyady Muhammad Ghufron , Larassati Larassati , Setiyono Setiyono , Subroto Gatot , Wijaya Ketut Anom , Wulanjari Distiana , Farisi Oria Alit , Basuki Basuki 
Published: March 2024

Vegetative propagation by cut-grafting has the advantage of being able to obtain seeds that inherit two superior traits from two scion clones in a relatively short time. The purpose of this research is to determine the effect of using orthotropic and plagiotropic rootstocks applied by Bacillus and Pseudomonas on the early growth of robusta coffee seedlings from cut grafting. The method used was by using a completely randomized factorial design with 2 factors and was repeated 3 times. The first factor was the use of rootstock cuttings which consisted of 2 levels, which were S1 (orthotropic stem) and S2 (plagiotropic stem). The second factor was the concentration of Bacillus and Pseudomonas which consisted of 5 levels, which were B0 (0 ml/L), B1 (20 ml/L), B2 (40 ml/L), B3 (60 ml/L) and B4 (80 ml/L). The results showed that (1) there was no interaction between the use of variatic rootstock and the application of the biological agent (2) The use of orthotropic rootstock increased plant growth, that is the number of primary roots and the number of leaves and (3) Application of the biological agent of Bacillus and Pseudomonas with concentrations of B4 increased seedling growth on all observed parameters except the number of shoots. Based on the results of this research, the benefit for coffee planters is to obtain alternative planting materials, namely from orthotropic branches. For further research, the use of orthotropic branch planting material as a scion plant can be investigated.
A influência da aparência nas decisões tomadas pelo tribunal do júri
Authors: Miyazaki Paula de Oliveira Santos , Silva Nelson Finotti , Miglioli Carlos Florido , Domingos Neide Aparecida Micelli 
Published: March 2024

A aparência física de uma pessoa pode ter um impacto sobre seu funcionamento e sobre a sua vida, podendo influenciar, inclusive, integrantes do poder judiciário em suas decisões. O objetivo deste estudo foi investigar a influência da aparência do réu na formação do convencimento de potenciais jurados. Estudo descritivo, transversal. Trinta (30) retratos (faces) obtidos por inteligência artificial foram retirados de dois sites que geram rostos artificiais a partir de combinação de fotos reais. Esses 30 retratos foram apresentados aos participantes, denominados juízes, que escolheram 10 retratos atraentes e 10 não atraentes. Em seguida, potenciais jurados responderam individualmente a um questionário sociodemográfico e, em seguida, foram solicitados a ler a descrição de um crime de homicídio fictício. Após a leitura, receberam os 10 retratos considerados atraentes e os 10 não atraentes indicados pelos juízes e foram informados que deveriam apontar o retrato do possível autor do crime. Participaram do estudo 391 potenciais jurados, a maioria do gênero feminino (277 / 71%). Quanto ao grau de escolaridade, a maioria tinha ensino superior completo (306 / 78,2%). A técnica de Escalonamento Multidimensional Não-Métrico gerou resultados de todas as percepções dos respondentes representadas por pontos (cada ponto é uma imagem). Os pontos próximos demonstraram a existência de uma relação entre as percepções dos respondentes, enquanto os pontos distantes apresentaram características diferentes quando comparados com os pontos próximos. Foram identificados dois pontos (retratos) distantes e isolados dos demais, ambos homens e considerados não atraentes (alto índice de não atratividade). Esses dois indivíduos receberam o maior número de votos SIM (considerado autor do crime) e têm alta porcentagem de não atratividade: 91,9% e 89,2% os consideraram não atraentes. Retratos com alto grau de não atratividade foram considerados autor do crime pela maioria dos participantes.
George Sclavunos (1869-1954): Anatomical Insights and his contribution into the “Magenstrasse of Waldeyer”
Authors: Verzoviti Ioanna , Saintanis Michail , Chrysikos Dimosthenis , Filippou Dimitrios , Schizas Dimitrios , Antonopoulos Ioannis K. , Troupis Theodore 
Published: March 2024

The purpose of this article is to present a well-known physician and highlight his contribution into an essential, but neglected anatomical feature. George Sclavunos (1869-1954) was a 20th century Greek physician, whose scientific work was a significant milestone in global medical knowledge. In 1899 he became Professor of Anatomy and Head Director of the Department of Anatomy. In 1906 Sclavunos G. published the first volume of the three volume book “Human Anatomy” (1906-1926), which is characterized by its unparalleled illustrations. For more than a century it was the most important book of medical literature in Greece. In 1926 he became a Full Member of the Academy of Athens and was named Life Partner of the International Anatomi- cal Society. His interests included Anatomy, Physiology, Histology, as well as Osteology and Syndesmology. In his book “Human Anatomy”, he described for first time the “Sialine Groove of the Stomach”, which was described by Waldeyer-Hartz almost at the same time as the “Magenstrasse”, a German word that means “stomach road”. It is a ribbon-like path that extends along the lesser curvature of the stomach from the gastric cardia to the antrum and releases the gastric content directly into the small intestine. Its importance is confirmed by its association not only with drug delivery, but also with anti-obesity surgical techniques. The old German term has come back into common medical usage in view of the commonly performed Magenstrasse and Mill pro- cedure, a form of bariatric surgery.
Conclusion: Sclavunos G. managed to observe an anatomical structure that has remained of great importance until today.
Satisfaction Level Spiritual Care of Coronary Heart Disease Patients Using Spiritual Assessment Tool
Authors: Wisuda Aris Citra , Sansuwito Tukimin bin , Suraya Citra , Rusmarita  
Published: March 2024

with using application of Spiritual Assessment Tool. This type of research is descriptive analytic with cross sectional approach. results In this study, it is known that the age of the respondents is mostly in the age range of (>35 Year old age), which is as much as 81.8%. Most of the patient education is low education which is as much as 72.7%. Patient Satisfaction Spiritual Care in the pre-test is known to be mostly stated as not satisfied, as much as 68.2%, and in the post-test, Patient Satisfaction Spiritual Care experienced a change in that the patient stated that he was satisfied as much as 90.9%. In conclusion, spiritual care can increase patient satisfaction, especially coronary heart disease sufferers at Siti Khadijah Islamic Hospital, Palembang in 2023.
Manganese and iron-doped yttrium borate as an excellent multifunctional inorganic material
Authors: Gupta Pankaj , Sahni Mohit 
Published: March 2024

Manganese and iron-doped π-YBO3 have been synthesized using a modified epoxide-mediated gel method. The PXRD pattern evaluated the formation of the desired phase and the structural changes. EDS spectra determined the elemental analysis of undoped and doped samples. Raman spectra observed the stretching and bending modes of B-O bonds. The direct band gaps for doped samples were 1.47 and 2.07 eV, respectively, lower than the band gap value of 5.81 eV for π-YBO3. The green and blue indigo emission bands were observed in the photoluminescence spectra. Doped samples showed good magnetic properties as they are antiferromagnetic and ferromagnetic at low temperature (T = 5 K) M-H plot and SQUID measurement. An indigenously built Sawyer-Tower circuit is used to measure ferroelectric hysteresis. Photodegradation studies of RhB were conducted under UV-visible irradiation.
Interpretation of Legal Verses According to the Shafi'i School through Ibn Hajar's "Tuhfat al-Muhtaj" and Al-Ramli's "Nihayat al-Muhtaj": A comparative analytical study
Authors: Algumaei Ammar 
Published: March 2024

The research focused on interpreting legal verses from the book of purification in Tuhfat al-Muhtaj by Ibn Hajar al-Haytami and Nihayat al-Muhtaj by al-Ramli, conducting a comparative analytical study of their interpretations. The significance of this research lies in its amalgamation of interpretations of legal verses from two fundamental texts, which are essential in understanding the relied-upon sources in Shafi'i jurisprudence, namely the conclusions of the eminent scholars Ibn Hajar and al-Ramli. This provides insight into the interpretation of legal verses within the framework of Shafi'i jurisprudence. The researcher arrived at several conclusions, including the following:

Jurisprudential texts often contain interpretations of legal verses and precise deductions that may not be found in many traditional exegesis texts.
The profound expertise of Ibn Hajar and al-Ramli in the science of interpretation, as well as their mastery in various fields beyond Shafi'i jurisprudence, is evident through their discussions on interpreting verses and responding to objections raised against their interpretations.
Analysis of Office Management at Government Agency Offices to Provide Public Services in Warudoyong Sukabumi
Authors: Ajeng Rosmaula  , Anggi Maulana  , Ai Restaria  , Muhamad Wildan Mailidan  , Tia Nuraini  , Alfin Fauzan  
Published: March 2024

Abstract
Office management in government agencies plays an important role as a management catalyst in achieving public services. Public service is an effort in providing services provided by an organization or agency in meeting customer needs in accordance with the principles and principles of public service. The purpose of this research is to analyze and understand office management and services in Warudoyong Sub-district, Sukabumi City. The research method used is descriptive research through a qualitative approach. The results of the research are that the Warudoyong Sub-district apparatus always works together in providing the best service to the community, besides that in the implementation of public services, service standards must be prepared by the organizer by taking into account the organizer's capabilities, community needs, and environmental conditions.
Keywords: Community, Service, Office, Warudoyong.
Update on the use of gamified educational resources in the development of cognitive skills
Authors: Gonzalez-Argote Javier , Castillo-González William , Hernández Estevez Jorge Ernesto 
Published: March 2024

Introduction: the use of game elements and game design techniques in content unrelated to the same is known as gamification. It is likely that gamified environments generate a fruitful scenario to evoke complex processes within higher nervous activity and thus develop cognitive skills. This facilitates its application for educational purposes. The objective was to characterize the use of gamified educational resources in the development of cognitive skills. Method: a total of 27 articles in Spanish and English were reviewed, from Pubmed, Scielo and Scopus; using as keywords: gamification, game, cognition, being more than 50% of the last five years.Result: gamification improves the services in which it is applied through playful experiences, it can positively influence aspects of the student experience such as level of interest, intellectual intensity and intrinsic motivation, by providing opportunities for autonomy, relationship and competition. There is a strong need for further exploration and experimentation to provide a gamified design that meets user preferences, tailored to individual characteristics. Conclusions: Gamified educational environments facilitate learning like a game. Through gamification it seems to increase motivation, commitment, active interaction, level of interest, critical thinking, intellectual intensity and intrinsic motivation. The effectiveness of gamification largely depends on the use of different game features and how they are implemented
PENGEMBANGAN MEDIA PEEMBELAJARAN (Kajian Tafsir dan Hadits Tarbawi)
Authors: Ahmad Hufron  
Published: March 2024

Media pembelajaran merupakan seperangkat alat yang dapat menyampaikan pesan-pesan dalam proses pembelajaran, dari penyampai pesan (pendidik) kepada penerima pesan (peserta didik) untuk mencapai tujuan yang telah ditetapkan secara efektif dan efisien. Landasan penggunaan media dalam pembelajaran harus dapat dilaksanakan dengan penuh tanggung jawab dan penuh hikmah, agar pendidik dan peserta didik dapat menjalin komunikasi yang baik, sehingga tercipta suasana edukatif yang kondusif. Sumber media pembelajaran dapat ditemukan pada al-Qur’an dan Hadits. Media pembelajaran dalam perspektif Al-Qur’an dan al Hadits, dapat dipandang dan diklasifikasikan menjadi media audio, visual dan audio visual. Sebagai seorang pendidik, Rasulullah SAW sangat memahami media yang bersifat baik dalam menyampaikan pengetahuan kepada manusia, beliau menjelaskan suatu informasi melalui gambar agar lebih mudah dipahami dan diserap oleh akal dan jiwa.
Promosi Pengenalan Kampung Adat Untuk Mempercepat Pengembangan Wilayah Melalui Pembangunan Kampung Tematik Kota Padang
Authors: Amnur Hidra , Yulherniwati  , Nova Fitri , Gusman Taufik , Amnur Adelwin 
Published: March 2024

Dalam mewujudkan masyarakat kota padang yang madani berbasis pendidikan, perdagangan, dan pariwisata. Pemerintah Kota Padang mempunyai program Padang beltline adalah srategi pembangunan kota padang melalui program unggulan yang dibingkai dalam satu kesatuan yang membentuk sabuk atau beltline. Padang beltline salah satunya meliputi Pengembangan kampung tematik. Kegiatan membangun Kampung Tematik dalam pengembangan ekonomi lokal masyarakat berdasarkan Potensi Wilayah. Salah Satu dari 11 kampung Tematik tersebut adalah Kampung Adat Kelurahan Sungai sarik Kecamatan Kuranji. Kuranji adalah sebuah kecamatan di Kota Padang, provinsi Sumatra Barat, Indonesia. Kecamatan ini meliputi Nagari Pauh IX yang terdiri dari sembilan tepian yaitu Ampang, Anduring, Gunung Sarik, Kalumbuk, Korong Gadang, Kuranji, Lubuk Lintah, Pasar Ambacang, dan Sungai Sapih. Nama kecamatan ini diambil dari salah satu tepian di Nagari Pauh IX, yaitu Kuranji, yang juga menjadi nama sungai yang membelah kecamatan ini, sungai Batang Kuranji. Potensi yang ada di Kuranji ini adalah Kampung Adat.Berdasarkan data dan informasi Kampung Adat sangat membutuhkan adanya media promosi dan publikasi digital maupun konvensional Kampung Adat sebagai kawasan wisata adat. Maka daripada itu diadakanlah Pengabdian masyarakat ini untuk memabantu program pemerintah kota Padang di Kampung Adat dengan membuat website sebagai media promosi dan publikasi digital
Mood Associated With Health- and Social Care–Related Quality of Life in Patients With Advanced Multiple Sclerosis
Authors: Ouwerkerk Maaike , Rietberg Marc B. , van der Linden Meike M. W. , Uitdehaag Bernard M. J. , van Wegen Erwin E. H. , de Groot Vincent 
Published: March 2024

ABSTRACT

BACKGROUND:
Individuals with advanced multiple sclerosis (MS) have complex care requirements and are more likely to use long-term facilities. This study determined the associations between mood and social care–related quality of life (SCRQOL), and health-related quality of life (HRQOL) and examined the association between HRQOL and SCRQOL.

METHODS:
Baseline data from a cohort study were used. Patients completed questionnaires, including the Hospital Anxiety and Depression Scale (HADS), Adult Social Care Outcomes Toolkit (ASCOT), and EuroQOL 5D-5L (EQ-5D-5L) and EQ-Visual Analogue Scale (EQ-VAS). Linear regression analyses were employed to assess the relationships between mood and both outcomes of QOL while controlling for relevant confounding factors (βs; 95% CI). The cross-sectional association between SCRQOL and HRQOL was examined using Pearson correlation coefficients (r).

RESULTS:
A total of 75 patients, with a mean age of 56.1 years and a disease duration of 17.3 years, were enrolled from a long-term care facility in the Netherlands. Results showed that after controlling for confounders, HADS is an independent determinant of ASCOT (βs = −.368; 95% CI, −.581 to −.154) and EQ-5D-5L (βs = −.297; 95% CI, −.507 to −.087). Also, there are significant but weak correlations between ASCOT and EQ-5D-5L (r = 0.242; 95% CI, .015-.468), between ASCOT and EQ-VAS (r = 0.230; 95% CI, .003-.457) and between EQ-5D-5L and EQ-VAS (r = 0.227; 95% CI, .000-.454).

CONCLUSIONS:
Mood, especially the depression component, is an important determinant of both HRQOL and SCRQOL in advanced MS. Focusing on mood in health care and social care may contribute to the improvement of QOL in a broader sense.
Role Played by Biochemical and Hematological Parameters in the Prediction of Cardiovascular Risk
Authors: Nuwanthika W. K. T. , Welivitigoda D. I. K. , Senadeera S. P. N. N. , Kottahachchi D. U. , Ranaweera C. B. , Wijesighe N. 
Published: March 2024

Introduction: The term “cardiovascular diseases” (CVDs) refers to any disease affecting the heart or blood vessels. CVDs are the most common cause of death worldwide and the most responsible reason for 10% of deaths in the early 20th century. These CVDs can emerge as myocardial infarction or ischemic heart disease, stroke, and congestive heart failure. For diagnosing CVDs, physical, radiological investigations, and laboratory investigations for cardiac enzymes and lipid profile are used. In clinical practices, cardiovascular risk prediction models are very important in the identification, prevention, and staging of the severity of CVDs. Framingham Risk Score-coronary heart disease, Framingham Risk Score-cardiovascular disease, QRESEARCH-cardiovascular risk algorithm, Joint British Society risk-calculator-3, WHO/ISH CVD risk prediction charts, and Atherosclerotic Cardiovascular Disease risk-estimator are some of the available CVD risk estimators. However, many of these estimators can be used only to evaluate individuals more than 40 years and to assess the risk for 10-years. Therefore, new risk estimators are needed to overcome the deficiencies of the available risk estimators. Research have been done in novel directions; on ratios of routinely performing hematological and biochemical laboratory parameters such as Neutrophil to Lymphocyte Ratio (NLR), Platelet to Lymphocyte Ratio (PLR), and Aspartate aminotransferase to Alanine aminotransferase ratio (AST/ALT). The outcomes indicate a relationship between the above-said ratios and the risk for CVDs.
Conclusion: On such grounds, this review describes the importance of developing a CVD risk estimator by amalgamating some of the biochemical and hematological parameters, including NLR, PLR, and AST/ALT ratios, aiming to overcome the existing shortcomings.
NMR Analysis of Thiodiglycol Oxidation by Mammalian Alcohol Dehydrogenases
Authors: Novak Mark J. , Brimfield Alan A. 
Published: September 2006

AbstractNuclear magnetic resonance spectroscopy (NMR) is a powerful technique for elucidating the metabolism of xenobiotics, as it allows for the least ambiguous assignment of chemical structure when compared to other forms of spectroscopy. In addition, it is a sensitive technique that can reveal the presence of transient species that otherwise would not be detected by utilizing either large‐scale batch processes or other forms of spectroscopic analyses. The primary focus of this unit describes the use of NMR to identify metabolites arising from the oxidation of thiodiglycol by equine and human variants of alcohol dehydrogenase (ADH). Given that it is often risky to base metabolism studies on a single form of spectroscopy, a spectrophotometric method is also presented. In addition, incorporation of independent organic syntheses in conjunction with the spectroscopic studies to further solidify structural identification of the ADH metabolites is presented.
Measurement of the Vitamin E Metabolites, Carboxyethyl Hydroxychromans (CEHCs), in Biological Samples
Authors: Leonard Scott W. , Traber Maret G. 
Published: September 2006

AbstractMetabolites of α‐ and γ‐tocopherol, 2,5,7,8‐tetramethyl‐2‐(2′‐carboxyethyl)‐6‐hydroxychroman (α‐CEHC) and 2,7,8‐trimethyl‐2‐(β‐carboxyethyl)‐6‐hydroxychroman (γ‐CEHC), respectively, are produced in the liver and have been measured in biological fluids and tissue. Compared to α‐tocopherol concentrations, metabolite concentrations are as much as a factor of a thousand lower, requiring extremely sensitive methodology to attain accurate measurements. This unit presents a protocol for CEHC extraction from biological samples, and describes very specific and sensitive HPLC/MS analysis.
Overview of Biacore Systems and Their Applications
Authors: Jason‐Moller Laure , Murphy Michael , Bruno JoAnne 
Published: September 2006

AbstractSurface plasmon resonance (SPR) allows for the investigation of the functional nature of binding interactions and provides detailed kinetic information across a wide range of molecular weights, including small molecules, all without the use of labels. Here the various Biacore instrument platforms and their primary uses, ranging from semi‐automated systems designed for simple, flexible basic research to fully automated, high‐throughput systems, and systems designed to function in regulated environments, are all highlighted. The available sensor chip surfaces and immobilization techniques are also discussed. Biacore SPR biosensors can be used for a wide variety of assays, including specificity, active concentration measurement, kinetics, and affinity and thermodynamic parameters. Biacore SPR biosensors, which measure real‐time analysis of biospecific interactions without the use of labeled molecules, can be used for a wide variety of protein interaction assays. In this unit, examples and recommendations for studying protein interactions with a variety of molecules are provided. This unit also shows how the technology can be used to determine binding specificity, active concentration measurements, and the determination of kinetic and thermodynamic parameters.