bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medicago truncatula as a Model for Nonhost Resistance in Legume-Parasitic Plant Interactions
Authors: Lozano-Baena M. Dolores , Prats Elena , Moreno M. Teresa , Rubiales Diego , Pérez-de-Luque Alejandro 
Published: August 2007

Abstract
Crenate broomrape (Orobanche crenata) is a root parasitic weed that represents a major constraint for grain legume production in Mediterranean and West Asian countries. Medicago truncatula has emerged as an important model plant species for structural and functional genomics. The close phylogenic relationship of M. truncatula with crop legumes increases its value as a resource for understanding resistance against Orobanche spp. Different cytological methods were used to study the mechanisms of resistance against crenate broomrape of two accessions of M. truncatula, showing early and late acting resistance. In the early resistance accession (SA27774) we found that the parasite died before a tubercle had formed. In the late resistance accession (SA4327) the parasite became attached without apparent problems to the host roots but most of the established tubercles turned dark and died before emergence. The results suggest that there are defensive mechanisms acting in both accessions but with a time gap that is crucial for a higher success avoiding parasite infection.
In Vivo Hexamerization and Characterization of the Arabidopsis AAA ATPase CDC48A Complex Using Förster Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy
Authors: Aker José , Hesselink Renske , Engel Ruchira , Karlova Rumyana , Borst Jan Willem , Visser Antonie J.W.G. , de Vries Sacco C. 
Published: August 2007

Abstract
The Arabidopsis (Arabidopsis thaliana) AAA ATPase CDC48A was fused to cerulean fluorescent protein and yellow fluorescent protein. AAA ATPases like CDC48 are only active in hexameric form. Förster resonance energy transfer-based fluorescence lifetime imaging microscopy using CDC48A-cerulean fluorescent protein and CDC48A-yellow fluorescent protein showed interaction between two adjacent protomers, demonstrating homo-oligomerization occurs in living plant cells. Interaction between CDC48A and the SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 (SERK1) transmembrane receptor occurs in very restricted domains at the plasma membrane. In these domains the predominant form of the fluorescently tagged CDC48A protein is a hexamer, suggesting that SERK1 is associated with the active form of CDC48A in vivo. SERK1 trans-phosphorylates CDC48A on Ser-41. Förster resonance energy transfer-fluorescence lifetime imaging microscopy was used to show that in vivo the C-terminal domains of CDC48A stay in close proximity. Employing fluorescence correlation spectroscopy, it was shown that CDC48A hexamers are part of larger complexes.
Signaling from an Altered Cell Wall to the Nucleus Mediates Sugar-Responsive Growth and Development inArabidopsis thaliana
Authors: Li Yunhai , Smith Caroline , Corke Fiona , Zheng Leiying , Merali Zara , Ryden Peter , Derbyshire Paul , Waldron Keith , Bevan Michael W. 
Published: August 2007

AbstractSugars such as glucose function as signal molecules that regulate gene expression, growth, and development in plants, animals, and yeast. To understand the molecular mechanisms of sugar responses, we isolated and characterized an Arabidopsis thaliana mutant, high sugar response8 (hsr8), which enhances sugar-responsive growth and gene expression. Light-grown hsr8 plants exhibited increased starch and anthocyanin and reduced chlorophyll content in response to glucose treatment. Dark-grown hsr8 seedlings showed glucose-hypersensitive hypocotyl elongation and development. The HSR8 gene, isolated using map-based cloning, was allelic to the MURUS4 (MUR4) gene involved in arabinose synthesis. Dark-grown mur1 and mur3 seedlings also exhibited similar sugar responses to hsr8/mur4. The sugar-hypersensitive phenotypes of hsr8/mur4, mur1, and mur3 were rescued by boric acid, suggesting that alterations in the cell wall cause hypersensitive sugar-responsive phenotypes. Genetic analysis showed that sugar-hypersensitive responses in hsr8 mutants were suppressed by pleiotropic regulatory locus1 (prl1), indicating that nucleus-localized PRL1 is required for enhanced sugar responses in hsr8 mutant plants. Microarray analysis revealed that the expression of many cell wall–related and sugar-responsive genes was altered in mur4-1, and the expression of a significant proportion of these genes was restored to wild-type levels in the mur4-1 prl1 double mutant. These findings reveal a pathway that signals changes in the cell wall through PRL1 to altered gene expression and sugar-responsive metabolic, growth, and developmental changes.
Allele-Specific Expression Patterns Reveal Biases and Embryo-Specific Parent-of-Origin Effects in Hybrid Maize
Authors: Springer Nathan M. , Stupar Robert M. 
Published: August 2007

AbstractWe employed allele-specific expression (ASE) analyses to document biased allelic expression in maize (Zea mays). A set of 316 quantitative ASE assays were used to profile the relative allelic expression in seedling tissue derived from five maize hybrids. The different hybrids included in this study exhibit a range of heterosis levels; however, we did not observe differences in the frequencies of allelic bias. Allelic biases in gene expression were consistently observed for ∼50% of the genes assayed in hybrid seedlings. The relative proportion of genes that exhibit cis- or trans-acting regulatory variation was very similar among the different genotypes. The cis-acting regulatory variation was more prevalent and resulted in greater expression differences than trans-acting regulatory variation for these genes. The ASE assays were further used to compare the relative expression of the B73 and Mo17 alleles in three tissue types (seedling, immature ear, and embryo) derived from reciprocal hybrids. These comparisons provided evidence for tissue-specific cis-acting variation and for a slight maternal expression bias in ∼20% of genes in embryo tissue. Collectively, these data provide evidence for prevalent cis-acting regulatory variation that contributes to biased allelic expression between genotypes and between tissues.
A Molecular Timetable for Apical Bud Formation and Dormancy Induction in Poplar
Authors: Ruttink Tom , Arend Matthias , Morreel Kris , Storme Véronique , Rombauts Stephane , Fromm Jörg , Bhalerao Rishikesh P. , Boerjan Wout , Rohde Antje 
Published: August 2007

AbstractThe growth of perennial plants in the temperate zone alternates with periods of dormancy that are typically initiated during bud development in autumn. In a systems biology approach to unravel the underlying molecular program of apical bud development in poplar (Populus tremula × Populus alba), combined transcript and metabolite profiling were applied to a high-resolution time course from short-day induction to complete dormancy. Metabolite and gene expression dynamics were used to reconstruct the temporal sequence of events during bud development. Importantly, bud development could be dissected into bud formation, acclimation to dehydration and cold, and dormancy. To each of these processes, specific sets of regulatory and marker genes and metabolites are associated and provide a reference frame for future functional studies. Light, ethylene, and abscisic acid signal transduction pathways consecutively control bud development by setting, modifying, or terminating these processes. Ethylene signal transduction is positioned temporally between light and abscisic acid signals and is putatively activated by transiently low hexose pools. The timing and place of cell proliferation arrest (related to dormancy) and of the accumulation of storage compounds (related to acclimation processes) were established within the bud by electron microscopy. Finally, the identification of a large set of genes commonly expressed during the growth-to-dormancy transitions in poplar apical buds, cambium, or Arabidopsis thaliana seeds suggests parallels in the underlying molecular mechanisms in different plant organs.
The Maize Floury1 Gene Encodes a Novel Endoplasmic Reticulum Protein Involved in Zein Protein Body Formation
Authors: Holding David R. , Otegui Marisa S. , Li Bailin , Meeley Robert B. , Dam Thao , Hunter Brenda G. , Jung Rudolf , Larkins Brian A. 
Published: August 2007

Abstract
The maize (Zea mays) floury1 (fl1) mutant was first reported almost 100 years ago, but its molecular identity has remained unknown. We report the cloning of Fl1, which encodes a novel zein protein body membrane protein with three predicted transmembrane domains and a C-terminal plant-specific domain of unknown function (DUF593). In wild-type endosperm, the FL1 protein accumulates at a high level during the period of zein synthesis and protein body development and declines to a low level at kernel maturity. Immunogold labeling showed that FL1 resides in the endoplasmic reticulum surrounding the protein body. Zein protein bodies in fl1 mutants are of normal size, shape, and abundance. However, mutant protein bodies ectopically accumulate 22-kD α-zeins in the γ-zein–rich periphery and center of the core, rather than their normal discrete location in a ring at outer edge of the core. The 19-kD α-zein is uniformly distributed throughout the core in wild-type protein bodies, and this distribution is unaffected in fl1 mutants. Pairwise yeast two-hybrid experiments showed that FL1 DUF593 interacts with the 22-kD α-zein. Results of these studies suggest that FL1 participates in protein body formation by facilitating the localization of 22-kD α-zein and that this is essential for the formation of vitreous endosperm.
Less conversation, more action: putting digital content creation at the heart of modern librarianship
Authors: McMenemy David 
Published: August 2007

PurposeTo propose that the notion of digital content creation needs to be more firmly perceived by the library community as an essential skill for all new entrants to the profession rather than an occasionally desirable one.Design/methodology/approachThe article provides a reflective viewpoint.FindingsThat digital content creation needs to be given a central plank in librarianship and that all new entrants to the profession should be taught the skills and knowledge necessary to manage digital creation projects. The paper also offers some observations regarding potential critical success factors for libraries engaged in digitisation projects.Practical implicationsThe article reflects on a specific aspect of practice, and thus may be of interest to practitioners who are involved in digital content creation in libraries.Originality/valueThe paper provides a reflective viewpoint on the skills modern library graduates should be taught, and as such may be of interest to library educators and practitioners.
Information storage and retrieval in the professional curriculum
Authors: Brunt Rodney 
Published: August 2007

PurposeTo reflect on the importance of information storage and retrieval in the library curriculum in the 1980s.Design/methodology/approachThe article provides a reflective viewpoint.FindingsThat cataloguing and classification was a vital skill for librarians to have, and that any moves to reduce it from the library school curriculum should be avoided.Practical implicationsShould be of interest to anyone interested in the historical competencies of librarianship and how they have been debated.Originality/valueOffers an interesting insight into historical discussions relating to an aspect of library education.
On reading “Information storage and retrieval in the professional curriculum” by Rodney Brunt
Authors: Poulter Alan , Brunt Rodney 
Published: August 2007

PurposeReflects on a paper extolling teaching cataloguing and indexing at library school written for Library Review in 1980.Design/methodology/approachReflective debate on the content of the original paper.FindingsThe sentiments of the original paper were supported, with the added proviso that nowadays the ability to organise information is a basic skill everyone needs.Practical implicationsMay be of interest to practitioners and library educators interested in this long standing debate.Originality/valuePaper carries a response by the original author to the reflections on his paper.
Application of information and communication technologies in special libraries in Kerala (India)
Authors: Haneefa Mohamed 
Published: August 2007

PurposeThis study was conducted to investigate the application of information and communication technologies (ICT) in special libraries in Kerala, India.Design/methodology/approachThe following methods were used to collect data for the study: questionnaire survey of librarians and library users, semi‐structured interviews with librarians, and observational visits in the libraries. This study was confined only to the automated special libraries in Kerala.FindingsThe analyses revealed that though the libraries had hardware, software, and communication facilities to some extent, ICT‐based resources and services were not reaching the users to the expected extent. Library automation in special libraries in Kerala was largely commenced during the period 1990‐2000. CDS/ISIS was used more in the libraries than any other software. The library catalogue found to be the most popular area for automation. The ICT‐based resource used by the largest percentage of the users was the e‐mail. Most of the libraries were hampered by lack of funds, lack of infrastructure, and lack of skilled professionals to embark on automation of all library management activities and application of ICT. A good number of the library users were not satisfied with the application of ICT in their libraries and indicated “inadequate ICT infrastructure” as their major reason for dissatisfaction. They proposed a variety of measures of formal orientation and training on ICT to become more effective users.Originality/valueThe study provides recommendations to enhance library automation and effective and efficient application of ICT.
A comparative assessment of urban and rural households' behaviors towards fresh red meat consumption
Authors: Yıldırım İbrahim , Ceylan Melike 
Published: August 2007

PurposeThe major aim of this study is to determine the effect of urbanization and income level on the behaviours of consumers towards red meat consumption structure in Van, Turkey.Design/methodology/approachThe sample size was found to be 96 and 95 households in urban and rural areas, respectively, using sampling applicable when the population is limited. The households were classified into four groups according to their average yearly incomes. Independent‐samples t‐test, one‐way ANOVA, χ2 and linear regression statistical tests were applied. The questionnaires were filled in between 15 November 2004 and 5 March 2005.FindingsThe average yearly fresh red meat consumption quantity per head of rural households (8.69 kg) was considerably lower than that of urban households (13.19 kg). The regression test showed that $1,000 increase in income would result in 4.38 and 8.24 kg increase in yearly red meat consumption quantity per households in urban and rural areas, respectively.Originality/valueThe paper compares the differences between the urban and rural areas in terms of consumers' behaviours towards fresh red meat consumption structure, which is original research subject for the region.
Nutrient composition of foods provided by school canteens in Bahrain
Authors: Musaiger Abdulrahman O. , Al‐Jedah Jassim H. , D'Souza Reshma 
Published: August 2007

PurposeThe aim of this study is to determine the nutritional profile of foods provided by the governmental school canteens in Bahrain.Design/methodology/approachNine types of foods commonly provided in 45 schools in Bahrain were analyzed for their proximate, mineral and heavy metal composition. Proximate composition was determined by measuring the moisture, ash, fat, protein (N × 6.25), crude fiber and carbohydrate content. Minerals and heavy metals were measured using flame or atomic absorption spectroscopy. Fatty acids were analyzed by gas liquid chromatography (GLC), as their methyl esters and sterols in the extracted fat were determined quantitatively by GLC.FindingsProximate analysis indicated protein content in the range of 8.14‐12.52 g/100 g, while fat content was below 10 g/100 g in all the foods, except thymes bakery (16.87 g/100 g). Consequently, thymes bakery also had higher energy values (376.15 kcal/100 g). Carbohydrates ranged between 33.86 and 47.29 g/100 g and fiber was high in the broad beans sandwich (1.16 g/100 g). Liver sandwich had considerable amount of iron (43.8 ppm), zinc (23.8 ppm) and potassium (3,360 ppm), while pizza was a good source of calcium (2,190 ppm). Lead was detected in 89 per cent of the foods, the highest being in pizza (0.234 ppm), being but contamination was well within the permitted limit. Aluminum was detected in 78 per cent of the foods, the highest level being observed in the chicken sandwich (10.8 ppm).Originality/valueThe results of this study can be utilized to improve the nutritive value of foods provided in the school feeding programme in the country.
Experimental investigation on system performance using palm oil as hydraulic fluid
Authors: Wan Nik W.B. , Maleque M.A. , Ani F.N. , Masjuki H.H. 
Published: August 2007

PurposeThe aim of this paper is to investigate hydraulic system performance using vegetable‐based palm oil as hydraulic fluid.Design/methodology/approachThe hydraulic system performance test at different operating conditions, such as pressure, speed and oil ageing, was performed using a Yuken vane pump test rig. The endurance system performance test was also conducted for 200 and 400 h. The effect of speed on flow slip coefficient in discrete and continuous tests was studied. In discrete testing, pressure of 35 and 200 bar and speed of 750 and 1,439 rpm were used in determining flow slip coefficient. The instantaneous data were recorded in a computer using an analog‐to‐digital data acquisition system with respect to time and the parameters stored were reservoir temperature, return line temperature, suction and delivery pressures, instantaneous flow rate, total flow, total running time and torque. The obtained results were interpolated for future prediction of the system performance.FindingsThe experimental and interpolated results showed that slip coefficient decreases with increasing pump speed. The effect of aging condition on volumetric efficiency showed that the efficiency increases with aging period due to increase in oil viscosity.Practical implicationsThis vegetable‐based palm oil could be a potentially useful substitute for mineral‐based energy transport media such as hydraulic fluid.Originality/valueThe investigation of hydraulic system performance using palm oil as hydraulic fluid is scarce in the literature. Therefore, the current study is quite new for the hydraulic system performance and it is hoped that it will provide a high value to researchers for further research before it can be used as hydraulic fluid.
Vibration and wear particles analysis in a test stand
Authors: Carlos Gonçalves Aparecido , Carvalho Cunha Rodrigo , Fabiano Lago Daniel 
Published: August 2007

PurposeThis paper aims to provide information on wear debris on oil and vibration analysis as predictive maintenance techniques.Design/methodology/approachThe estate of a reducer is verified by analyzing the vibration and oil conditions of a test rig under some variables.FindingsAccording to the vibration and oil analysis it was found what was happening in the reducer without disassembling it.Originality/valueThis paper gives information about two predictive maintenance techniques with a test rig.
Evaluating organisation core competences and associated personal competencies using analytical hierarchy process
Authors: Hafeez Khalid , Essmail Essmail Ali 
Published: August 2007

PurposeThis paper seeks to introduce an integrated framework to determine the relationships between organisation core competences and associated personal competencies.Design/methodology/approachAt first organisation core competences are determined by conducting internal and external benchmarking exercises, respectively, employing the “collectiveness” and “uniqueness” measures using key capabilities as a basis. Subsequently a pair‐wise comparison using the Analytic Hierarchy Process (AHP) is conducted to assess related personal competencies using the Chartered Institute of Personnel and Development list of competencies. The paper shows how these individual competencies are crucial to the overall organisational core competences. The framework is tested for a construction company, where five management directors are interviewed to develop an overall picture regarding the strengths and weaknesses of the company's key capabilities.FindingsOverall the results show that, despite being a construction company, the composition of its organisational capabilities is dominantly governed by intangible assets’ contributions. The AHP prioritisation analysis confirmed that with regard to Innovative solutions, organisational core competence, Customer focus, and Team orientation are the most related personal competencies.Originality/valueThis paper makes an original contribution in the core competence literature by showing how to evaluate individual competencies that are essential to the construction of the organisational core competence. The use of AHP facilitates consistent data by screening out any subjective anomalies. The outcome of this exercise can help management prioritise the most related personal competencies needed for developing its organisational core competences and to undertake crucial business functions.
A multidimensional measure of employees’ intangibles
Authors: Longo Mariolina , Mura Matteo 
Published: August 2007

PurposeThe aims of this research are: to develop and test an evaluation model of the intangible resources related to the employees; to value the impact that these intangible resources have on individual work attitudes such as job satisfaction and turnover propensity.Design/methodology/approachTwo surveys were conducted in 2005 and 2006 for employees of a leading Italian company in the agricultural food sector. A structural equation modelling methodology was used to define the quantitative measures of the system of resources and to determine the relations among the different constructs.FindingsIt was found that the items adopted in the study capture the constructs of human capital and structural capital and, moreover, these two dimensions positively affect individual work attitudes such as job satisfaction and turnover.Originality/valueThe measurement system developed in this research can be used in any firm that seeks to measure the intangible resources related to employees. The system represents a tool that may support managers in planning and control of human resource investments of the firm. One of the main limitations is that the study is based on surveys of employees, so it only enables comparison among different departments, production plants, professional category or seniority within the company. Moreover, the indicators used are not measured objectively, making it difficult for any inter‐company comparison.