bluuebunny/crossref_metadata_2025_split huggingface dataset:
An evaluation of serum blood parameters and amyloid A levels in pregnant women with threatened miscarriage
Authors: Isenlik Bekir Sitki , Sarica Mehmet Cihan , Kaygun Bilgesu Cetinel , Inal Hasan Ali 
Published: February 2024

AbstractObjectiveThis study investigated whether serum amyloid A (AA) levels can be used as a biomarker in patients with threatened abortion.Material and MethodsThis prospective cohort study was conducted at the Antalya Training and Research Hospital, Department of Obstetrics and Gynecology, Türkiye, between April and October 2023. Eighty‐eight pregnant women, 44 diagnosed with threatened miscarriage (Group 1) and 44 healthy individuals (Group 2), were included in the study. Sociodemographic, obstetric, and laboratory parameters were compared between the groups.ResultsNo statistically significant differences were observed between the groups in terms of sociodemographic data (age, body mass index, education level, economic status, occupation status, smoking, and alcohol consumption). However, obstetric characteristics (number of pregnancies, living children, miscarriages, dilatation and curettage, gestational age on admission, and fetal crown‐rump length) and laboratory values including complete blood count, hematocrit, leukocyte, neutrophil, lymphocytes, platelet, hs‐C‐reactive protein, neutrophil‐lymphocyte and platelet‐lymphocyte ratio (p > .05), and serum AA values (7.49 ± 3.07 in Group 1 vs. 9.46 ± 4.80 in Group 2, p = .024) differed significantly. Receiver operating characteristic analysis showed that the area under the curve (AUC: 0.662) was statistically significant for serum AA (p = .032), with a cut‐off value of ≥7.51 (95% [confidence interval] 0.516−0.749, sensitivity 65%, specificity 51%). The positive predictive value of serum AA for threatened miscarriage was 56.8%, and the negative predictive value 59.4%.ConclusionThis study shows that serum AA can be used as a biomarker in the diagnosis of threatened miscarriage. Prospective studies involving more participants are now needed to confirm our results.
Transgenerational effects of paternal exposures: the role of germline de novo mutations
Authors: Dehghanbanadaki Hojat , Jimbo Masaya , Fendereski Kiarad , Kunisaki Jason , Horns Joshua J , Ramsay Joemy M. , Gross Kelli X. , Pastuszak Alexander W. , Hotaling James M. 
Published: February 2024

AbstractGermline de novo mutations (DNMs) refer to spontaneous mutations arising during gametogenesis, resulting in genetic changes within germ cells that are subsequently transmitted to the next generation. While the impact of maternal exposures on germline DNMs has been extensively studied, more recent studies have begun to highlight the increasing importance of the effects of paternal factors. In this review, we have summarized the existing literature on how various exposures experienced by fathers affect the germline DNM burden in their spermatozoa, as well as their consequences for semen analysis parameters, pregnancy outcomes, and offspring health. A growing body of literature supports the conclusion that advanced paternal age (APA) correlates with a higher germline DNM rate in offspring. Furthermore, lifestyle choices, environmental toxins, assisted reproductive techniques (ART), and chemotherapy are associated with the accumulation of paternal DNMs in spermatozoa, with deleterious consequences for pregnancy outcomes and offspring health. Ultimately, our review highlights the clear importance of the germline DNM mode of inheritance, and the current understanding of how this is affected by various paternal factors. In addition, we explore conflicting reports or gaps of knowledge that should be addressed in future research.
Absorbable antibiotic beads for treatment of LVAD driveline infections
Authors: Wadiwala Ishaq , Garg Pankaj , Alamouti‐Fard Emad , Landolfo Kevin , Sareyyupoglu Basar , Ahmed Magdy El‐Sayed , Jacob Samuel , Pham Si 
Published: February 2024

AbstractBackgoundInfections of the left ventricular assist device (LVAD) driveline are a dreaded complication that results in high mortality and morbidity.MethodWe retrospectively reviewed five consecutive patients with severe continuous‐flow LVAD (HVAD, Heartmate 2, and Heartmate 3) driveline infection. These infections, which developed on an average of 960.4 ± 843.9 days after LVAD placement, were refractory to systemic antibiotics and local wound care. All were treated with extensive surgical debridement, local installation of absorbable antibiotic‐loaded calcium sulfate beads (vancomycin and tobramycin), primary wound closure, and 6 weeks of systemic antibiotics after surgery.ResultsFour patients had resolution of DLI, and one had a recurrent infection at another part of the driveline 7 months after the complete resolution of the previous site. This patient was successfully treated with debridement and bead placements. Three patients still have their LVADs, while two received orthotopic heart transplants. At the time of the transplant, there was no evidence of gross infection of the LVAD drivelines or pumps. At the average follow‐up time of 425.8 ± 151 days, no patients have an active infection.ConclusionTreatment of LVAD driveline infection with absorbable antibiotic beads with primary wound closure is a viable option and merits further investigation.
Editorial: Towards optimal thiopurine therapy for inflammatory bowel disease
Authors: van Liere Elsa L. S. A. , de Boer Nanne K. H. 
Published: February 2024

LINKED CONTENTThis article is linked to Vasudevan et al papers. To view these articles, visit https://doi.org/10.1111/apt.17831 and https://doi.org/10.1111/apt.17895
Investigating the effect of supplementing different levels of Isochrysis galbana on in vitro rumen fermentation parameters
Authors: Salehian Zahra , Khalilvandi‐Behroozyar Hamed , Pirmohammadi Rasoul , Ahmadifard Nasrollah , Almasi Hadi , Ramin Mohammad 
Published: February 2024

AbstractThis study aimed to investigate the effect of supplementing Isochrysis galbana (I. galbana) at levels of 0 (control), 1, 2, 3, 4, and 5 (g/100 g DM) of the diet on the gas production kinetics, methane production, rumen fermentation parameters, and relative microbial population in vitro. Supplementation of I. galbana at high level (5 g/100 g DM) caused a significant decrease in total gas production (p < 0.05). High supplementation rates (4 and 5 g/100 g DM) decreased CH4 production relative to the control by 18.4% and 23.2%, respectively. Although rumen ammonia nitrogen (N‐NH3) and total volatile fatty acids (VFA) concentrations were affected by dietary treatments, but the VFA profile did not changed. The relative proportion of protozoa and methanogenic archaea as well as Anaerovibrio lipolytica, Prevotella spp., Ruminococcus flavefaciens, and Fibrobacter succinogenes were decreased significantly as a result of microalgae supplementation. However, the relative abundance of Ruminococcus albus, Butyrivibrio fibrisolvens and Selenomonas ruminantium were significantly increased (p < 0.05), related to the control group. As well, the pH was not affected by dietary treatments. It was concluded that I. galbana reduced in vitro CH4 production and methanogenic archaea that its worth to be investigated further in in vivo studies.
Recent advances of oocyte/embryo vitrification in mammals from rodents and large animals
Authors: Kamoshita Maki , Sugita Hibiki , Kageyama Atsuko , Kawata Yui , Ito Junya , Kashiwazaki Naomi 
Published: February 2024

AbstractVitrification is a valuable technology that enables semipermanent preservation and long‐distance or international transportation of genetically modified and native animals. In laboratory mice, vitrification maintains and transports embryos, and many institutions and companies sell vitrified embryos. In contrast, despite numerous papers reporting on vitrification in livestock over the past decade, practical implementation has yet to be achieved. However, with advances in genome editing technology, it is anticipated that the number of genetically modified domestic animals will increase, leading to a rise in demand for vitrification of oocytes and embryos. Here, we provide an objective overview of recent advancements in vitrification technology for livestock, drawing a comparison with the current developments in laboratory animals. Additionally, we explore the future prospects for vitrification in livestock, focusing on its potential benefits and drawbacks.
Etoposide combined with cytarabine and pegfilgrastim for poorly mobilizing patients with multiple myeloma and lymphoma: A prospective multicentre study
Authors: Ye Peipei , Cheng Yixuan , Lian Jiaying , Tong Hongyan , Li Linjie , Guo Qunyi , Zhu Weiguo , Feng Weiying , Huang Li , Shou Lihong , Chen Dong , Liu Xuhui , Li Shuangyue , Du Xiaohong , Yang Min , Yu Wenjuan , Qian Jiejing , Hu Chao , Wang Huafeng , Jin Yangjin , Shen Jian , Hong Pan , Pei Renzhi , Jin Jie , Lu Ying 
Published: February 2024

SummaryA chemotherapy‐based mobilization regimen in patients who mobilize poorly, based on etoposide, cytarabine and pegfilgrastim (EAP), has recently been introduced. The aim of this prospective study was to investigate the efficacy and safety of the EAP regimen in patients with poorly mobilizing multiple myeloma (MM) or lymphoma. This single‐arm clinical trial was performed at eight public hospitals in China and was registered as a clinical trial (NCT05510089). The inclusion criteria were; (1) diagnosis of MM or lymphoma, (2) defined as a ‘poor mobilizer’ and (3) aged 18–75 years. The EAP regimen consisted of etoposide 75 mg/m2/day on days 1–2, cytarabine 300 mg/m2 every 12 h on days 1–2 and pegfilgrastim 6 mg on day 6. The primary endpoint of the study was the ratio of patients achieving adequate mobilization (≥2.0 × 106 CD34+ cells/kg). From 1 September 2022 to 15 August 2023, a total of 58 patients were enrolled, 53 (91.4%) achieved adequate mobilization, while 41 (70.7%) achieved optimal mobilization with a median number of cumulative collected CD34+ cells was 9.2 (range 2.1–92.7) × 106/kg and the median number of apheresis per patient of 1.2. The median time from administration of the EAP regimen to the first apheresis was 12 days. Approximately 8.6% of patients required plerixa for rescue, which was successful. Twelve (20.7%) of the 58 patients suffered grade 2–3 infections, while 25 (43.1%) required platelet transfusions. The duration of neutrophil and platelet engraftment was 11 days. In conclusion, these results suggest that the EAP mobilization regimen might be a promising option for poorly mobilizing patients with MM or lymphoma.
Isoliquiritigenin attenuates neuroinflammation after subarachnoid hemorrhage through inhibition of NF‐κB‐mediated NLRP3 inflammasome activation
Authors: Wenfei Zhang , Xiang Tao , Chen Chen , Yang Tao , Yun Tao , Zhibiao Chen , Ge Zhang 
Published: February 2024

AbstractNeuroinflammation contributes to neurological dysfunction in the patients who suffer from subarachnoid hemorrhage (SAH). Isoliquiritigenin (ISL) is a bioactive component extracted from Genus Glycyrrhiza. This work is to investigate whether ISL ameliorates neuroinflammation after SAH. In this study, intravascular perforation of male Sprague–Dawley rats was used to establish a SAH model. ISL was administered by intraperitoneal injection 6 h after SAH in rats. The mortality, SAH grade, neurological score, brain water content, and blood–brain barrier (BBB) permeability were examined at 24 h after the treatment. Expressions of tumor necrosis factor‐α, interleukin‐6, Iba‐1, and MPO were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR). Besides, the expression levels of NF‐κB p65 and NLRP3, ASC, caspase‐1, IL‐1β, and IL‐18 were analyzed by western blot. The experimental data suggested that ISL treatment could ameliorate neurological impairment, attenuate brain edema, and ameliorate BBB injury after SAH in rats. ISL treatment repressed the expression of proinflammatory cytokines TNF‐α and IL‐6, and meanwhile inhibited the expression of Iba‐1 and MPO. ISL also repressed NF‐κB p65 expression as well as the transport from the cytoplasm to the nucleus. In addition, ISL significantly suppressed the expression levels of NLR family pyrin domain containing 3 (NLRP3), ASC, caspase‐1, IL‐1β, and IL‐18. These findings suggest that ISL inactivates NLRP3 pathway by inhibiting NF‐κB p65 translocation, thereby repressing the neuroinflammation after SAH, and it is a potential drug for the treatment of SAH.
Myopia progression following 0.01% atropine cessation in Australian children: Findings from the Western Australia – Atropine for the Treatment of Myopia (WA‐ATOM) study
Authors: Lee Samantha Sze‐Yee , Nilagiri Vinay Kumar , Lingham Gareth , Blaszkowska Magdalena , Sanfilippo Paul G. , Franchina Maria , Clark Antony , Mackey David A. 
Published: February 2024

AbstractBackgroundA rebound in myopia progression following cessation of atropine eyedrops has been reported, yet there is limited data on the effects of stopping 0.01% atropine compared to placebo control. This study tested the hypothesis that there is minimal rebound myopia progression after cessation of 0.01% atropine eyedrops, compared to a placebo.MethodsChildren with myopia (n = 153) were randomised to receive 0.01% atropine eyedrops or a placebo (2:1 ratio) daily at bedtime during the 2‐year treatment phase of the study. In the third year (wash‐out phase), all participants ceased eyedrop instillation. Participants underwent an eye examination every 6 months, including measurements of spherical equivalent (SphE) after cycloplegia and axial length (AL). Changes in the SphE and AL during the wash‐out phase and throughout the 3 years of the study (treatment + wash‐out phase) were compared between the treatment and control groups.ResultsDuring the 1‐year wash‐out phase, SphE and AL progressed by −0.41D (95% CI = −0.33 to −0.22) and +0.20 mm (95% CI = −0.46 to −0.36) in the treatment group compared to −0.28D (95% CI = 0.11 to 0.16) and +0.13 mm (95% CI = 0.18 to 0.21) in the control group. Progression in the treatment group was significantly faster than in the control group (p = 0.016 for SphE and <0.001 for AL). Over the 3‐year study period, the cumulative myopia progression was similar between the atropine and the control groups.ConclusionsThese findings showed evidence of rapid myopia progression following cessation of 0.01% atropine. Further investigations are warranted to ascertain the long‐term effects of atropine eyedrops.
Critical appraisal of YouTube videos regarding peri‐implant diseases and conditions: A cross‐sectional analysis
Authors: Gaikwad Amit , Rachh Pranidhipurnima , Raut Kaustubh , Nadgere Jyoti 
Published: February 2024

AbstractPurposeTo critically appraise the quality and reliability of YouTube videos regarding peri‐implant diseases and conditions as a source of information for patients, students, and young clinicians.Materials and MethodsIn March 2023, electronic searches were performed on YouTube website to identify videos related to peri‐implant diseases and conditions. We considered only the relevant 250 English‐language videos with durations between 3 and 30 min for final analyses. Following the eligibility criteria videos were evaluated for their demographic data, including number of views; number of likes, dislikes, and comments; days since upload; duration; and number of subscribers. Moreover, two assessors independently evaluated the quality and reliability of the included videos using the DISCERN and Video Information and Quality Index (VIQI) tools. Statistical analyses were performed using the Kruskal–Wallis test and Spearman correlation analysis (∝ = 0.05).ResultsA total of 69 videos were included for profound analyses. The average DISCERN and VIQI scores were 35.04 ± 6.3 and 14.18 ± 2.46, with 53 videos categorized as “poor” quality using the DISCERN tool. A Spearman rank correlation analysis presented a strong agreement between the DISCERN and VIQI scores (r = .753; p < .001). Nevertheless, based on different sources of upload, no statistically significant differences were reported for video demographics, interaction index, and DISCERN and VIQI scores.ConclusionsAlthough YouTube videos on peri‐implant diseases and conditions present accurate preliminary information, their reliability still remains uncertain. Hence, we urge respective policymakers to recognize, endorse and produce high‐quality videos for accurate information dissemination.
Training and assessment for colorectal surgery and appendicectomy– a systematic review
Authors: Hertz Peter , Rattenborg Søren , Haug Tora R. , Houlind Kim , Konge Lars , Bjerrum Flemming 
Published: February 2024

AbstractAimThere is currently an increased focus on competency‐based training, in which training and assessment play a crucial role. The aim of this systematic review is to create an overview of hands‐on training methods and assessment tools for appendicectomy and colon and rectal surgery procedures using either an open, laparoscopic or robot‐assisted approach.MethodA systematic review of Medline, Embase, Cochrane and Scopus databases was conducted following the PRISMA guidelines. We conducted the last search on 9 March 2023. All published papers describing hands‐on training, evaluation of performance data and development of assessment tools were eligible. The quality of studies and the validity evidence of assessment tools are reported.ResultsFifty‐one studies were identified. Laparoscopic assessment tools are abundant, but the literature still lacks good‐quality assessment tools for open appendicectomy, robotic colectomy and open rectal surgery. Overall, there is a lack of discussion regarding the establishment of pass/fail standards and the consequences of assessment. Virtual reality simulation is used more for appendicectomy than colorectal procedures. Only a few of the studies investigating training were of acceptable quality. There is a need for high‐quality studies in open and robotic‐assisted colon surgery and all approaches to rectal surgery.ConclusionThis review provides an overview of current training methods and assessment tools and identifies where more research is needed based on the quality of the studies and the current validity evidence.
Peri‐operative and long‐term outcomes of kidney transplantation in patients with cystic fibrosis
Authors: O'Kelly John A. , Alaya Fatimah , Davis Niall F. , Little Dilly , Mohan Ponnusamy 
Published: February 2024

AbstractIntroductionThere is a lack of data regarding the peri‐operative and long‐term outcomes of kidney transplantation in cystic fibrosis (CF) patients. Herein, we report the peri‐operative and long‐term outcomes of kidney transplantation in CF patients.Materials and methodsAll CF patients who received a kidney transplant at the national kidney transplant center between 1993 and 2022 were identified. Recipients of the contralateral donor kidney were selected as a control group. Primary outcomes included 1‐, 5‐, and 10‐ year death‐censored graft survival and overall survival. Secondary outcomes included peri‐operative morbidity, acute graft rejection, delayed graft function (DGF), and length of stay (LOS).ResultsFourteen patients received a kidney transplant over the study period. Median age at transplantation was 35 (IQR 31, 40) years. The 1‐, 5‐, and 10‐year death‐censored graft survival was 92, 74, and 74% in the CF group compared to 100, 92, and 92% in the control group (p = .44). The 1‐, 5‐, and 10‐year overall survival in the CF group was 85, 66, and 57% compared to 100, 92, and 82% in the control group (p = .036). There was no significant difference in peri‐operative outcomes including LOS (10 vs. 11 days, p = .84), ICU admission (1 vs. 0 patients, p > .99), acute rejection episodes (2 vs. 1 patients, p > .99), and DGF (1 vs. 2 patients, p = .60).ConclusionCF patients have good long‐term graft survival, however, overall survival was worse compared to a matched cohort. These data provide important information for transplant surgeons when considering suitable donor allografts in this unique patient population.
Mitral E‐wave to stroke volume ratio displays stronger diagnostic performance to identify elevated left ventricular filling pressures than mitral E/e’ during passive leg lift: A cross‐sectional study employing simultaneous echocardiography and catheterization
Authors: Venkateshvaran Ashwin , Lindqvist Per 
Published: February 2024

AbstractBackgroundElevated filling pressure is a hallmark of heart failure (HF) and portends poor prognosis. Accurate diagnosis is challenging, given that patients with normal filling pressure at rest develop disproportionate elevation with sudden preload increase. We aimed to test the accuracy of the ratio between mitral inflow velocity (E) and left ventricular stroke volume (SV) to identify patients with elevated filling pressure with passive leg lifting (PLL) and compare this with other echocardiographic surrogates of filling pressure.MethodsDoppler echocardiography and right heart catheterization (RHC) were simultaneously performed in 37 patients (11 males, mean age 67 ± 12 years) with exertional dyspnea. Twenty‐six healthy controls (14 males, mean age 60 ± 12 years) were added as reference. SV, cardiac output (CO), tricuspid regurgitation peak gradient (TRG), mitral E‐wave (E) and early myocardial velocity (e’) were obtained at rest and with PLL. E/SV, E/CO and E/e’ were calculated and correlated with invasive pulmonary capillary wedge pressures (PCWP) with PLL.ResultsDuring PLL, E/SV (AUC = 0.94) displayed stronger diagnostic ability to identify PCWP >15 mmHg than E/e’ (AUC = 0.81), mitral E/A ratio (0.76) and resting invasive PCWP (0.84). An E/SV cutoff of >1.0 showed 88% sensitivity and 75% specificity to identify elevated PCWP. Further, 10 patients (27%) were reassigned during PLL from normal to postcapillary pulmonary hypertension (postCPH), and 6 patients (16%) switched diagnosis from precapillary PH (preCPH) to postCPH.ConclusionThe novel E/SV ratio identifies patients with elevated PCWP with PLL and displays stronger diagnostic performance than routinely utilized echocardiographic measures such as E/e’ in addition to resting, catheterization derived PCWP.