bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monoclonal antibody Ber‐EP4: Its use in the differential diagnosis of malignant mesothelioma and carcinoma in cell blocks of malignant effusions and FNA specimens
Authors: Maguire Brigid , Whitaker Darrel , Carrello Salvatore , Spagnolo Dominic 
Published: February 2007

AbstractFormal sublimate‐fixed cell blocks derived from 129 malignant pleural (and some peritoneal) effusions, 8 benign effusions with reactive mesothelial cells, and 23 FNA specimens, were immunostained with monoclonal antibody Ber‐EP4 to assess its ability to distinguish malignant mesothelioma (MM) from carcinoma.Only 2 of 44 (4%) well‐characterized MM were Ber‐EP4+, while none of 8 benign mesothelial proliferations reacted with the antibody. Fifty‐seven percent of 23 pulmonary adenocarcinomas (AC) and 60% of 43 pulmonary carcinomas of all other histological types were Ber‐EP4+. Of 40 metastatic AC originating from breast, gastrointestinal tract, ovary, endometrium, and kidney, 80% were Ber‐EP4+. The predictive value of positive Ber‐EP4 staining in distinguishing AC from MM was 96%. The predictive value of a negative Ber‐EP4 in excluding MM was 70%, when the differential diagnosis was adenocarcinoma. These results suggest that Ber‐EP4 is helpful in differentiating MM and AC if used together with other discriminating antibodies. © 1994 Wiley‐Liss, Inc.
Merkel cell tumor of the eyelid and the cytologic aspect in fine‐needle aspirates: Report of a case
Authors: Pérez‐Guillermo M. , Sola‐Pérez J. , Abad‐Montaño C. , Quirante F. A. Pastor , Montalb Romero M. S. Án 
Published: February 2007

AbstractWe describe one case of Merkel cell tumor (MCT) of the eyelid diagnosed by fine‐needle aspiration (FNA). The main cytologic features were: isolated cells, loose cohesive sheets, and rosette‐like structures in a hematic background. The cells were round to ovoid and very uniform. Chromatin was finely granular and frequent mitotic figures were observed. Under close scrutiny small faintly stained juxtanuclear “caps” were seen. A strong dot‐like positivity for CK22 and NFP was observed in the position corresponding to the “caps.” NSE positivity was diffuse, although a weak dot‐like positivity was seen in some cells. S‐100 was negative. Both the cytologic features and the immunocytologic profile of Merkel cell turmor are very characteristic. FNA enables an early and confident diagnosis of this aggressive tumor and an early planning of surgery. © 1994 Wiley‐Liss, Inc.
Small cell anaplastic carcinoma of the prostate: Report of a case with positive urine cytology
Authors: Nguyen‐Ho Phong , Nguyen Gia‐Khanh , Villanueva Ricci R. 
Published: February 2007

AbstractA case of small‐cell anaplastic carcinoma of the prostate, occurring in an 82‐year‐old man with prior prostatic adenocarcinoma, is reported. The exfoliated tumor cells in the urine were seen singly or in loose aggregates with molding. They showed scant cytoplasm and slightly pleomorphic, hyperchromatic nuclei with inconspicuous nucleoli. By electron microscopy the tumor consisted of primitive epithelial cells without glandular or neuroendocrine diffrentiation. The tumor pursued a highly aggressive course, and the patient died of metastatic disease 4 mo after the diagnosis. © 1994 Wiley‐Liss, Inc.
Role of fine‐needle biopsy in an atypical case of lupus lymphadenopathy
Authors: Loo Christine K. C. , Greenberg Merle L. 
Published: February 2007

AbstractNecrotizing lymphadenitis dominated by individual cell necrosis with tingible body macrophages and karyorrhectic nuclear debris is an unusual appearance in smears from fine‐needle biopsies (FNB) of lymph nodes (Greenberg et al., Diagn Cytopathol 1993; 9:443‐447). These features together with a polymorphous population of lymphocytes, absent epithelioid histiocytes and neutrophils, suggest a possible diagnosis of either histiocytic necrotizing lymphadenitis (HNL) (syn. Kikuchi's Disease) or systemic lupus erythematostis (SLE). These were the cytologic features found in a FNB of a cervical lymph node in a 43‐year‐old female, treated for a mixed connective tissue disease. A presumptive diagnosis of an acute lupus lymphadenitis was made, based on the above features, and was subsequently confirmed by the presence of raised antinuclear antibodies as well as resolution of the lymphadenopathy on high dose steroids. © 1994 Wiley‐Liss, Inc.
Cytomegalovirus sialadenitis in patients with the acquired immunodeficiency syndrome: A potential diagnostic pitfall with fine‐needle aspiration cytology
Authors: Wax Tim D. , Layfield Lester J. , Zaleski Sue , Bhargara Vivek , Cohen Michael , Lyerly Herbert K. , Fisher Samuel R. 
Published: February 2007

AbstractWe report three cases of cytomegalovirus (CMV) sialadenitis which presented as parotid gland nodules in patients infected with the human immunodeficiency virus. While CMV is known to widely infect patients with the acquired immunodeficiency syndrome (AIDS), we are aware of only a single report of CMV sialadenitis in a patient with AIDS (Pialoux et al.: Rev Infect Dis 1991;13:338). Utilizing fine‐needle aspiration (FNA) cytology as the initial investigative modality, two cases were correctly diagnosed preoperatively while the third case displayed atypical features and was interpreted erroneously as carcinoma leading to surgical intervention. Upon review of these cases of CMV sialadenitis, the characteristic intranuclear inclusions are best identified with Papanicolaou (Pap) staining. CMV sialadenitis should be considered in the differential diagnosis of painless salivary gland enlargement in patients with AIDS. We believe this lesion can be diagnosed preoperatively with FNA and the interpretation is aided by evaluating both Pap‐ and Giemsa‐stained material. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of suture granulomas of the breast: A potential pitfall in the cytologic diagnosis of recurrent breast cancer
Authors: Maygarden Susan J. , Novotny Debra B. , Johnson Danna E. , Powers Celeste N. , Frable William J. 
Published: February 2007

AbstractThe fine‐needle aspirates of three cases of suture granulomas of the breast area following mastectomy, lumpectomy, or axillary node dissection were reviewed. The original histologic diagnoses were mucinous (colloid) carcinoma, intraductal carcinoma, and low‐grade phyllodes tumor. In two patients a new nodule developed in the surgical scar, and in the third a nodule developed in the axilla 2 cm away from the scar. The cellularity of the aspirates ranged from low to moderate, and all three cases contained variable numbers of spindled cells and fragments of cellular stroma. Multinucleated giant cells characteristic of suture granulomas were absent in all cases. Abundant metachromatic amorphous background material was present in one case, which was interpreted as recurrent mucinous carcinoma. The remaining cases were interpreted as suspicious for recurrent neoplasm. Upon excision, all were suture granulomas with fibroblastic proliferation. Suture granulomas can both clinically and cytologically mimic recurrent malignancy. The predominance of spindled cells and dissimilarity to the original tumor appear to be the most helpful features to cytologically distinguish suture granuloma from recurrent carcinoma in the absence of the characteristic multinucleated giant cells. © 1994 Wiley‐Liss, Inc.
Women in the early days of cytology: A personal recollection
Authors: Koprowska Irena 
Published: February 2007

AbstractThe fight for the acceptance of the validity of cytopathology has been fought by a group of individuals associated with George N. Papanicolaou. The common effort to convince the skeptics that Papanicolaou's method of diagnosing cancer was the best way to detect its presence at an early stage became a bond linking all of us. When we taught cytology, we felt more like preaching a gospel than like teaching a subject. Those of us who became involved in original research experienced an additional excitement from expanding the frontiers of our new specialty. This uniquely stimulating experience has been shared by women and men in most of the early cytology teaching centers throughout the world. This was diffrent from the enthusiasm with which people enter the field of cytopathology today. All of us had to constantly prove the validity of what we were doing, whereas now the value of cytologic diagnosis is fully recognized and taken for granted. © 1994 Wiley‐Liss, Inc.
Polyomavirus in the urine: Follow‐up study
Authors: Minassian Haig , Schinella Roger , Reilly Jane C. 
Published: February 2007

AbstractThis study was undertaken to determine the significance of polyomavirus nuclear inclusions in urine cytology specimens. Thirty‐two such cases were identified and patient follow‐up was instituted. We have determined that there is no detectable urologic damage in such patients. In a minority of cases inclusions were found in subsequent urines but they ultimately disappeared within 3 mo. In a minority of the patients (35 percent) there was evidence of diseases associated with immunosuppression or administration of immunosuppressive drugs. © 1994 Wiley‐Liss, Inc.
Paranuclear blue inclusions in small cell undifferentiated carcinoma: A diagnostically useful finding demonstrated in fine‐needle aspiration biopsy smears
Authors: Walker William P. , Wittchow Richard J. , Bottles Kent , Layfield Lester J. , Hirschowitz Sharon , Cohen Michael B. 
Published: February 2007

AbstractIn a study of bone marrow aspiration smears. Wittchow et al. (Mod Pathol 1992;5:555–558) described a highly characteristic finding, paranuclear blue inclusions (PBIs), found almost exclusively in cases of metastatic small cell undifferentiated carcinoma (SCUC). PBIs are 1–4 μm, light blue, cytoplasmic inclusions best visualized with Romanowsky‐type stains. These inclusions are most easily found indenting the nuclei within clusters of closely opposed tumor cells. In the current study air‐dried fine‐needle aspiration biopsy (FNAB) smears from 146 primary and meta‐static small cell and non‐small cell adult and childhood malignancies were reviewed. PBIs were found in 28/32 (88%) of SCUC but were observed in only 4/44 (9%) non‐small cell carcinomas, 2/21 (9.5%) lymphomas, 1/8 (12.5%) melanomas, 0/14 sarcomas, and 6/27 (22%) small round cell neoplasms. These results suggest that the presence of PBIs in air‐dried FNAB smears of adult neoplasms, while not pathognamonic of SCUC, are a diagnostically useful finding. PBIs may be seen in a variety of different childhood small round cell neoplasms which limits their utility in this setting. The recognition of PBIs is most important to the cytologist who may not have access to ancillary studies, such as immunohistochemistry and electron microscopy. © 1994 Wiley‐Liss, Inc.
Paragonimiasis in a cystic breast mass: Case report and implications for examination of aspirated cyst fluids
Authors: Fogel Steven P. , Chañdrasoma Parakrama T. 
Published: February 2007

AbstractWe report a case of paragonimiasis diagnosed by fine‐needle aspiration of a breast mass in a middle‐aged Korean female with a history of breast silicone injections. Although parasitoses have been discovered by aspiration of cyst fluid in otherwise sterile sites (i.e., liver and lung), the cystic presentation in the breast of a middle‐aged female complicated the diagnosis in this case. Some authors have argued against the routine cytologic examination of non‐bloody cyst fluids in premenopausal women, but this protocol may lead to a false negative diagnosis in the rare instance of a parasitic cyst. While microscopic examination of breast cyst fluid is probably not warranted in every patient, it may be useful in patients at risk for unusual cystic lesions, especially if clinical and mammo‐graphic signs of fibrocystic change are absent. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy: Pitfalls in the diagnosis of spindle‐cell lesions
Authors: Powers Celeste N. , Berardo Melora D. , Frable William J. 
Published: February 2007

AbstractSeven cases of spindle‐cell proliferations in which fine‐needle aspiration biopsy (FNAB) did not correlate with subsequent histology are presented. Three cases were considered low‐grade sarcoma, one a dermatofibrosarcoma protuberans (DFSP), one a spindle‐cell tumor with malignancy not excluded, and one a rhab‐domyosarcoma vs. a fibrosarcoma. Two of the these three were histologically nodular fasciitis and one an inflammatory pseudotumor. Two cases were diagnosed cytologically as fibromatosis or nodular fasciitis (NF). One of these histologically was an intramuscular hemangioma, the other a DESP. The last two cases were diagnosed by FNAB as spindle‐cell lesion, undetermined if benign or malignant, and malignant fibrous histiocytoma (MFH). Histologically both of these case were leiomyosarcoma. The cyto‐logic features of each case, differential diagnosis, and potential pitfalls are discussed. In the evaluation of FNAB smears dominated by spindle cells, cellullarity, individual cells and cell patterns,and background stromal features coupled with a precise clinical history may allow a narrow differential diagnosis with a focus on whether the lesion is benign or malignant. Caution is warranted in the exact classification of spindle‐cell tumors from FNAB as this may have a major impact on patient management. © 1994 Wiley‐Liss, Inc.
Immunocytochemical typification of mesothelial cells in effusions: In vivo and in vitro models
Authors: Ferrandez‐lzquierdo A. , Navarro‐Fos S. , Gonzalez‐Devesa M. , Gil‐Benso R. , Llombart‐Bosch A. 
Published: February 2007

AbstractWe have performed immunocytochemical, immunoelectron microscopy. Western blot, and culture techniques using monoclonal antibodies against cytokeratin, vimentin, and desmin on 17 benign and 20 malignant effusions of pleural and ascitic origin. Triple coexpression of these three antigens was observed in benign reactive mesothelial cells as well as in one case of mesothelioma. All metastatic adenocarcinoma cells were consistently negative to desmin and positive to cytokeratin and vimentin. Present results were helpful to distinguish reactive and malignant mesothelioma from metastatic carcinoma cells in effusions. © 1994 Wiley‐Liss, Inc.
Prognostic and diagnostic implications of estrogen and progesterone receptor assays in cytology
Authors: Masood Shahla 
Published: February 2007

AbstractScientific evidence suggests that cancer is a consequence of genetic changes that result in uncontrolled cellular growth, tissue invasion, and metastasis. This concept has led to a great deal of excitement in the medical community in the hope that precise identification of genes involved in human cancer could potentially facilitate our understanding of human malignancies. It may also be possible to apply this information directly to different clinical settings. Many technologies are now available which offer opportunities for pathologists to not only expand their diagnostic abilities, but also to assess the prognosis of a given tumor in a specific patient. These include immunocytochemistry, flow cytometry, cell image analysis, and molecular genetic studies. In this review, the role of immunocytochemical hormone receptor assay as an aid in tumor prognosis and in prediction of tumor response to endocrine therapy will be discussed. Emphasis will be placed on the use of hormone receptor assay as an adjunct in diagnostic pathology and in assessment of metastatic tumors of unknown origin. The potential value of assessment of hormone receptors in different cytologic preparations will also be discussed. © 1994 Wiley‐Liss, Inc.
Prevalence of HPV 16 and 18 DNA sequences in CIN III lesions of adults and adolescents
Authors: Johnson Terri L. , Joseph Christine L. M. , Caison‐Sorey T. Jann , Smith Richard E. , Bedrossian Carlos W. M. , Sarkar Fazlul H. 
Published: February 2007

AbstractAdolescents may be more susceptible to cervical human papillomavirus (HPV) infections and may have more rapid progression of cervical intraepithelial neoplastic (CIN) lesions than adults. We evaluated Papanicolaou (Pap) smears and cervical tissue specimens from a consecutive series of 25 adolescent (age 15‐20 yr) and 17 adult (age 35‐40 yr) patients with a histologic diagnosis of CIN III. The study patients were all Detroit residents enrolled in a health maintenance organization (HMO) affliated with Henry Ford Hospital. The cervical tissue specimens were evaluated for HPV 6b/11, HPV 16, and HPV 18 using agarose gel electrophoresis and Southern hybridization following polymerase chain reaction (PCR) DNA amplification. While the small sample size precluded testing for statistical significance, HPV 16 and/or HPV 18 DNA was detected in specimens from 21/25 (84%) adolescents compared to 12/17 (71%) adults (odds ratio [OR] = 2.2; 95% confidence interval [CI] = 0.49‐9.74). The relationship between adolescence and HPV infections appears to be stronger for HPV 18 and mixed HPV 16/18 infections (OR = 5.6; 95% CI = 0.7‐42.4) than for HPV 16 infections (OR = 1.93; 95% CI = 0.4‐8.8). None of the cervical specimens contained HPV 6b/11 DNA. Oral contraceptive (OC) use was associated with HPV infection in patients with CIN III, but there was no association between cigarette smoking and HPV infection. The effect of OC use on the relationship of age and HPV could not be evaluated due to small sample size. The effects of previous sexually transmitted disease (STD) on the relationship of age and HPV were assessed. Among women with a history of STD, there was a strong association between HPV and adolescent age (OR = 18.0; 95% CI = 1.2‐260.0). Our data suggest that among women with CIN III, adolescents have a higher prevalence of certain high‐risk types of HPV infections than adults. The excess is due predominantly to the higher rates of HPV 18 and mixed HPV 16/18 infections in adolescents. The positive relationship between high‐risk HPV infections and young age was most evident in adolescents with a history of STD. The results from this study suggest that differences in HPV type infections may be related to the more aggressive clinical course of CIN in adolescents. © 1994 Wiley‐Liss, Inc.
DNA adduct assay in cervical epithelium
Authors: Simons Andrew M. , Phillips David H. , Coleman Dulcie V. 
Published: February 2007

AbstractNumerous epidemiological studies have shown that there is an association between smoking and cervical cancer. However, the essential evidence to show whether this relationship is casual or causal is lacking. The demonstration of DNA modification by tobacco components in the cervical epithelium would provide biochemical evidence to support a causal role. In this study, DNA from 39 cervical biopsies was analysed for the presence of DNA adducts using the 32P‐postlabelling technique. A questionnaire on smoking habit and a urinary cotinine assay were used to identify smokers and nonsmokers. DNA samples from smokers [identified from questionnaire] were found to have significantly higher adduct levels than nonsmokers (Mann‐Whitney one‐tailed U‐test, 95% CI > 0.339, P = 0.024). Exclusion of the women whose urinary cotinine levels did not confirm their self‐reported smoking status (smoker or nonsmoker) increased this significance (95% CI > 0.508, P = 0.01). Women who had abnormal cervical smears hadsignificantly higher DNA adduct levels than those with normal smears (95% CI > 0.439, P = 0.015). Monitoring of women with high DNA adduct levels may be a way of identifying women at risk of cervical cancer.These findings demonstrate that tobacco smoking by women leads to elevated levels of DNA adducts in cervical epithelium and provides the biochemical evidence to support the concept that smoking is a cause of cervical cancer. © 1994 Wiley‐Liss, Inc. Published 1994 Wiley‐Liss, Inc. © 1994 Wiley‐Liss, Inc.
Transthoracic fine‐needle aspiration biopsy cytology of pulmonary neoplasms
Authors: Hayes Malcolm M. M. , Zhang Dong Yue , Brown William 
Published: February 2007

AbstractThe experience with 212 transthoracic fine‐needle aspiration biopsy cytology specimens of mass lesions of the lung at a small Canadian hospital is reviewed. The technique showed a sensitivity of 78.0%, a specificity of 85.7%, a positive predictive value of 98.5%, and a negative predictive value of 25.0% when the cytological diagnoses were compared with the final clinicopathological diagnoses listed in the patients' charts. There was only one false positive diagnosis of malignancy. Diagnoses based on cytology of the aspirates were compared with histological diagnoses in 127 cases and the accuracy of typing of the major groups of carcinoma was assessed. Distinction between small‐cell carcinoma and non‐small‐cell carcinoma was usually accurate and fairly good correlation was achieved for all major types of carcinoma. The results for typing of the neoplasm are not as good as those from larger centres in the world. The technique is considered to be useful even in the setting of a small hospital. © 1994 Wiley‐Liss, Inc.
Paranuclear blue inclusions: An aid in the cytopathologic diagnosis of primary and metastatic pulmonary small‐cell carcinoma
Authors: Mullins Rejeana K. , Thompson Sophie K. , Coogan Philip S. , Shurbaji M. Salah 
Published: February 2007

AbstractAccurate diagnosis of small‐cell carcinoma of the lung (SCLC) is clinically important because of the therapeutic implications. SCLC must be distinguished from non‐small‐cell carcinoma (NSCLC) and lymphoma. Paranuclear blue inclusions (PBIs) were recently described as a feature of metastatic SCLC on air‐dried Wright‐stained bone marrow aspirate smears. To determine the utility of PBIs in distinguishing SCLC from NSCLC and lymphoma, we evaluated air‐dried Diff‐Quik‐stained smears from 103 fine‐needle aspiration (FNA) specimens and 14 touch imprint specimens. PBIs were identified in 24 (89%) of 27 cases of SCLC, in 6 (9%) of 64 non‐small‐cell carcinomas (P < 0.00001), and in two (8%) of the 26 lymphoma cases (P < 0.00001). No PBIs were seen on any of the alcohol‐fixed Papanicolaou or hematoxylin‐eosin (H&E) stained smears examined. In conclusion, PBIs appear to be a feature of SCLC on air‐dried cytologic material stained with Romanowsky type stains. In the presence of cytologic features of SCLC, the identification of PBIs provides a useful diagnostic feature for diferentiating between SCLC and NSCLC carcinomas, and between SCLC and lymphomas in FNA specimens and touch imprints from surgical specimens. © 1994 Wiley‐Liss, Inc.
Potassium permanganate resistant amyloid in fine‐needle aspirate of the liver
Authors: Srinivasan Radhika , Nijhawan Raje , Gautam Upasana , Bambery Pradeep 
Published: February 2007

AbstractA case of primary amyloidosis, initially detected by fine‐needle aspiration of the liver, is reported here. Amorphous acellular metachromatic material was seen extracellularly in between the hepatocytic cords compressing them. This material showed typical apple‐green birefringence under crossed bipolars after alkaline Congo‐red staining proved its amyloid nature. It was resistant to potassium permanganate pretreatment, indicating it to be of the AL type. © 1994 Wiley‐Liss, Inc.
Cervicovaginal cytology and the diagnosis of Chlamydia Trachomatis: A comparison with immunofluorescent results
Authors: Caudill Jill L. , Humphrey Sandra K. , Goellner John R. 
Published: February 2007

AbstractTo evaluate the accuracy of a Chlamydia diagnosis in our laboratory, cytologic Chlamydia diagnoses for the years 1990 and 1991 were compared with immunofluorescent studies for Chlamydia for the same period. Only one of 22 cases diagnosed cytologically as suggestive of Chlamydia had a positive immunofluorescent result. On rescreen of 110 cervicovaginal smears taken within I mo of a positive fluorescent antibody test, 11 cases were found to contain criteria suggestive of Chlamydia. These correlations represent a 4.5% specificity and 10% sensitivity rate, suggesting that our current efforts in cytology are not effective in determining the presence of Chlamydia. Diagn Cytopathol 1994; 11:20–22. © 1994 Wiley‐Liss, Inc.