bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute Infantile Gastroenteritis Associated with Human Enteric Viruses in Tunisia
Authors: Sdiri-Loulizi Khira , Gharbi-Khélifi Hakima , de Rougemont Alexis , Chouchane Slaheddine , Sakly Nabil , Ambert-Balay Katia , Hassine Mouna , Guédiche Mohamed Neji , Aouni Mahjoub , Pothier Pierre 
Published: February 2008

ABSTRACT
This prospective study, conducted from January 2003 to June 2005, investigated the incidence and the clinical role of various enteric viruses responsible for infantile gastroenteritis in 632 Tunisian children presenting in dispensaries (380 children) or hospitalized (252 children) for acute diarrhea. At least one enteric virus was found in each of 276 samples (43.7%). A single pathogen was observed in 234 samples, and mixed infections were found in 42 samples. In terms of frequency, rotavirus and norovirus were detected in 22.5 and 17.4% of the samples, respectively, followed by astrovirus (4.1%), Aichi virus (3.5%), adenovirus types 40 and 41 (2.7%), and sapovirus (1.0%). The seasonal distribution of viral gastroenteritis showed a winter peak but also an unusual peak from May to September. The severity of the diarrhea was evaluated for hospitalized infants. No significant differences were observed between rotavirus and norovirus infections with regard to the incidence and the clinical severity of the disease, especially in dehydration.
Prosthetic Hip Infection Caused by
Tropheryma whipplei
Authors: Cremniter Julie , Bauer Thomas , Lortat-Jacob Alain , Vodovar Dominique , Le Parc Jean-Marie , Emile Jean-François , Franc Brigitte , Sebbag Pierre , Gaillard Jean-Louis , Heym Beate 
Published: February 2008

ABSTRACT

We report a case of prosthetic hip infection due to
Tropheryma whipplei
in a 74-year-old man not previously known to have Whipple's disease. Diagnosis was based on systematic 16S rRNA gene amplification and sequencing of samples obtained during revision hip arthroplasty.
Validation of Virulence and Epidemiology DNA Microarray for Identification and Characterization of
Staphylococcus aureus
Isolates
Authors: Spence Richard P. , Wright Victoria , Ala-Aldeen Dlawer A. A. , Turner David P. , Wooldridge Karl G. , James Richard 
Published: February 2008

ABSTRACT

The human pathogen
Staphylococcus aureus
is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presence or absence of a large number of virulence-associated genes simultaneously in a single assay. We have developed and validated a novel, cost-effective multiwell microarray for the identification and characterization of
Staphylococcus aureus
. The array comprises 84 gene targets, including species-specific, antibiotic resistance, toxin, and other virulence-associated genes, and is capable of examining 13 different isolates simultaneously, together with a reference control strain. Analysis of
S. aureus
isolates whose complete genome sequences have been determined (Mu50, N315, MW2, MRSA252, MSSA476) demonstrated that the array can reliably detect the combination of genes known to be present in these isolates. Characterization of a further 43
S. aureus
isolates by the microarray and pulsed-field gel electrophoresis has demonstrated the ability of the array to differentiate between isolates representative of a spectrum of
S. aureus
types, including methicillin-susceptible, methicillin-resistant, community-acquired, and vancomycin-resistant
S. aureus
, and to simultaneously detect clinically relevant virulence determinants.
Vaccinia Virus E2L Null Mutants Exhibit a Major Reduction in Extracellular Virion Formation and Virus Spread
Authors: Domi Arban , Weisberg Andrea S. , Moss Bernard 
Published: February 2008

ABSTRACT
The vaccinia virus E2L (VACWR058) gene is conserved in all sequenced chordopoxviruses and is predicted to encode an 86-kDa protein with no recognizable functional motifs or nonpoxvirus homologs. Although the region immediately upstream of the open reading frame lacked optimal consensus promoter motifs, expression of the E2 protein occurred after viral DNA replication. Transfection studies, however, indicated that the promoter was weak compared to well-characterized intermediate and late promoters. The E2 protein was present in mature virions purified from infected cells but was more abundant in extracellular enveloped forms. Despite the conservation of the E2L gene in chordopoxviruses, deletion mutants could be isolated from both the WR and IHD-J strains of vaccinia virus. These null mutants produced very small plaques in all cell lines tested, reduced amounts of mature infectious virions, and very low numbers of extracellular virions. Nevertheless, viral protein synthesis appeared qualitatively and quantitatively normal. The defect in extracellular virus formation was corroborated by electron microscopy, which also showed some aberration in the wrapping of virions by cisternal membranes. Extracellular virions that did form, however, were able to induce actin tail formation.
Identification of a GP64 Subdomain Involved in Receptor Binding by Budded Virions of the Baculovirus
Autographica californica
Multicapsid Nucleopolyhedrovirus
Authors: Zhou Jian , Blissard Gary W. 
Published: February 2008

ABSTRACT

Enveloped virus entry into host cells is typically initiated by an interaction between a viral envelope glycoprotein and a host cell receptor. For budded virions of the baculovirus
Autographa californica
multicapsid nucleopolyhedrovirus, the envelope glycoprotein GP64 is involved in host cell receptor binding, and GP64 is sufficient to mediate low-pH-triggered membrane fusion. To better define the role of GP64 in receptor binding, we generated and characterized a panel of antisera against subdomains of GP64. Eight subdomain-specific antisera were generated, and their reactivities with GP64 proteins and neutralization of virus infectivity and binding were examined. Antibodies directed against the N-terminal region of GP64 (amino acids 21 to 159) showed strong neutralization of infectivity and effectively inhibited binding of
35
S-labeled budded virions to Sf9 cells. In addition, we generated virions displaying truncated GP64 constructs. A construct displaying the N-terminal 274 amino acids (residues 21 to 294) of the ectodomain was sufficient to mediate virion binding. Additional studies of antisera directed against small subdomains revealed that an antiserum against a 40-amino-acid region (residues 121 to 160) neutralized virus infectivity. Site-directed mutagenesis was subsequently used for functional analysis of that region. Recombinant viruses expressing GP64 proteins with single amino acid substitutions within amino acids 120 to 124 and 142 to 148 replicated to high titers, suggesting that those amino acids were not critical for receptor binding or other important GP64 functions. In contrast, GP64 proteins with single amino acid substitutions of residues 153 and 156 were unable to substitute for wild-type GP64 and did not rescue a
gp64
knockout virus. Further analysis showed that these substitutions substantially reduced binding of recombinant virus to Sf9 cells. Thus, the amino acid region from positions 21 to 159 was identified as a putative receptor binding domain, and amino acids 153 and 156 appear to be important for receptor binding.
Alveolar Macrophages Are a Major Determinant of Early Responses to Viral Lung Infection but Do Not Influence Subsequent Disease Development
Authors: Pribul Philippa K. , Harker James , Wang Belinda , Wang Hongwei , Tregoning John S. , Schwarze Jürgen , Openshaw Peter J. M. 
Published: February 2008

ABSTRACTMacrophages are abundant in the lower respiratory tract. They play a central role in the innate response to infection but may also modulate excessive inflammation. Both macrophages and ciliated epithelial cells respond to infection by releasing soluble mediators, leading to the recruitment of innate and adaptive effector cells. To study the role of lung macrophages in acute respiratory viral infection, we depleted them by the inhalation of clodronate liposomes in an established mouse model of respiratory syncytial virus (RSV) disease. Infection caused an immediate local release of inflammatory cytokines and chemokines, peaking on day 1, which was virtually abolished by clodronate liposome treatment. Macrophage depletion inhibited the activation (days 1 to 2) and recruitment (day 4) of natural killer (NK) cells and enhanced peak viral load in the lung (day 4). However, macrophage depletion did not affect the recruitment of activated CD4 or CD8 T cells, weight loss, or virus-induced changes in lung function. Therefore, lung macrophages play a central role in the early responses to viral infection but have remarkably little effect on the adaptive response occurring at the time of peak disease severity.
Alveolar Macrophages Are Indispensable for Controlling Influenza Viruses in Lungs of Pigs
Authors: Kim Heui Man , Lee Young-Won , Lee Ki-Ja , Kim Hyun Soo , Cho Sung Whan , van Rooijen Nico , Guan Yi , Seo Sang Heui 
Published: February 2008

ABSTRACT

Alveolar macrophages constitutively reside in the respiratory tracts of pigs and humans. An in vivo role of alveolar macrophages in defending against influenza viruses in mice infected with a reassorted influenza virus, 1918 HA/NA:Tx/91, was reported, but there has been no report on an in vivo role of alveolar macrophages in a natural host such as a pig using currently circulating human influenza virus. Here we show that in vivo depletion of alveolar macrophages in pigs by dichloromethylene diphosphonate (MDPCL2) treatment results in 40% mortality when pigs are infected with currently circulating human H1N1 influenza viruses, while none of the infected control pigs died. All infected pigs depleted of alveolar macrophages suffered from more severe respiratory signs than infected control pigs. Induction of tumor necrosis factor alpha in the infected pigs depleted of alveolar macrophages was significantly lower than that in the lungs of infected control pigs, and the induction of interleukin-10, an immunosuppressive cytokine, significantly increased in the lungs of infected pigs depleted of alveolar macrophages compared to infected control pigs. When we measured antibody titers and CD8
+
T lymphocytes expressing gamma interferon (IFN-γ), lower antibody titers and a lower percentage of CD8
+
T lymphocytes expressing IFN-γ were detectable in MDPCL2-treated infected pigs than in phosphate-buffered saline- and liposome-treated and infected pigs. Taken together, our findings suggest that alveolar macrophages are essential for controlling H1N1 influenza viruses in pigs.
Impact of an Updated Carbon Bond Mechanism on Predictions from the CMAQ Modeling System: Preliminary Assessment
Authors: Sarwar Golam , Luecken Deborah , Yarwood Greg , Whitten Gary Z. , Carter William P. L. 
Published: February 2008

Abstract
An updated and expanded version of the Carbon Bond mechanism (CB05) has been incorporated into the Community Multiscale Air Quality (CMAQ) modeling system to more accurately simulate wintertime, pristine, and high-altitude situations. The CB05 mechanism has nearly 2 times the number of reactions relative to the previous version of the Carbon Bond mechanism (CB-IV). While the expansions do provide more detailed treatment of urban areas, most of the new reactions involve biogenics, toxics, and species potentially important to particulate formation and acid deposition. Model simulations were performed using the CB05 and the CB-IV mechanisms for the winter and summer of 2001. For winter with the CB05 mechanism, ozone, aerosol nitrate, and aerosol sulfate concentrations were within 1% of the results obtained with the CB-IV mechanism. Organic carbon concentrations were within 2% of the results obtained with the CB-IV mechanism. However, formaldehyde and hydrogen peroxide concentrations were lower by 25% and 32%, respectively, during winter with the CB05 mechanism. For the summer, ozone concentrations increased by 8% with the CB05 mechanism relative to the CB-IV mechanism. The aerosol sulfate, aerosol nitrate, and organic carbon concentrations with the CB05 mechanism decreased by 8%, 2%, and 10%, respectively. The formaldehyde and hydrogen peroxide concentrations with the CB05 mechanism were lower by 12% and 47%, respectively, during summer. Model performance with the CB05 mechanism improved at high-altitude conditions and in rural areas for ozone. Model performance also improved for organic carbon with the CB05 mechanism.
The Identification and Verification of Hazardous Convective Cells over Oceans Using Visible and Infrared Satellite Observations
Authors: Donovan Michael F. , Williams Earle R. , Kessinger Cathy , Blackburn Gary , Herzegh Paul H. , Bankert Richard L. , Miller Steve , Mosher Frederick R. 
Published: February 2008

Abstract
Three algorithms based on geostationary visible and infrared (IR) observations are used to identify convective cells that do (or may) present a hazard to aviation over the oceans. The performance of these algorithms in detecting potentially hazardous cells is determined through verification with Tropical Rainfall Measuring Mission (TRMM) satellite observations of lightning and radar reflectivity, which provide internal information about the convective cells. The probability of detection of hazardous cells using the satellite algorithms can exceed 90% when lightning is used as a criterion for hazard, but the false-alarm ratio with all three algorithms is consistently large (∼40%), thereby exaggerating the presence of hazardous conditions. This shortcoming results in part from the algorithms’ dependence upon visible and IR observations, and can be traced to the widespread prevalence of deep cumulonimbi with weak updrafts but without lightning over tropical oceans, whose origin is attributed to significant entrainment during ascent.
Surface Wind Regionalization in Complex Terrain
Authors: Jiménez P. A. , García-Bustamante E. , González-Rouco J. F. , Valero F. , Montávez J. P. , Navarro J. 
Published: February 2008

AbstractDaily wind variability in the Comunidad Foral de Navarra in northern Spain was studied using wind observations at 35 locations to derive subregions with homogeneous temporal variability. Two different methodologies based on principal component analysis were used to regionalize: 1) cluster analysis and 2) the rotation of the selected principal components. Both methodologies produce similar results and lead to regions that are in general agreement with the topographic features of the terrain. The meridional wind variability is similar in all subregions, whereas zonal wind variability is responsible for differences between them. The spectral analysis of wind variability within each subregion reveals a dominant annual cycle and the varying presence of higher-frequency contributions in the subregions. The valley subregions tend to present more variability at high frequencies than do higher-altitude sites. Last, the influence of large-scale dynamics on regional wind variability is explored by studying connections between wind in each subregion and sea level pressure fields. The results of this work contribute to the characterization of wind variability in a complex terrain region and constitute a framework for the validation of mesoscale model wind simulations over the region.
Transport of Atmospheric Aerosol by Gap Winds in the Columbia River Gorge
Authors: Green Mark C. , Xu Jin , Adhikari Narendra 
Published: February 2008

Abstract
Typical diurnal wind patterns and their relationship to transport of atmospheric aerosol in the Columbia River gorge of Oregon and Washington are addressed in this paper. The measurement program included measurements of light scattering by particles (bsp) with nephelometers, and wind speed and direction, temperature, and relative humidity at seven locations in the gorge. Winds are shown to respond to along-gorge pressure gradients, and five common patterns were identified: strong, moderate, and light westerly (west to east), light easterly, and winter easterly. The strong westerly and winter easterly patterns were the most common summer and winter patterns, respectively, and represented strong gap flow. The light westerly and light easterly patterns occurred most frequently in spring and autumn transition periods. Winter easterly had the highest light scattering and indicated sources east of the gorge mainly responsible for haze. During summer, as westerly winds increased diurnally, a pulse of hazy air from the Portland, Oregon, metropolitan area is transported eastward into the gorge, arriving later with distance into the gorge. During light easterly flow impacts to haze from the city of The Dalles, Oregon, are noted as the wind shifts direction diurnally.
Seasonal and Synoptic Variations in Near-Surface Air Temperature Lapse Rates in a Mountainous Basin
Authors: Blandford Troy R. , Humes Karen S. , Harshburger Brian J. , Moore Brandon C. , Walden Von P. , Ye Hengchun 
Published: February 2008

Abstract
To accurately estimate near-surface (2 m) air temperatures in a mountainous region for hydrologic prediction models and other investigations of environmental processes, the authors evaluated daily and seasonal variations (with the consideration of different weather types) of surface air temperature lapse rates at a spatial scale of 10 000 km2 in south-central Idaho. Near-surface air temperature data (Tmax, Tmin, and Tavg) from 14 meteorological stations were used to compute daily lapse rates from January 1989 to December 2004 for a medium-elevation study area in south-central Idaho. Daily lapse rates were grouped by month, synoptic weather type, and a combination of both (seasonal–synoptic). Daily air temperature lapse rates show high variability at both daily and seasonal time scales. Daily Tmax lapse rates show a distinct seasonal trend, with steeper lapse rates (greater decrease in temperature with height) occurring in summer and shallower rates (lesser decrease in temperature with height) occurring in winter. Daily Tmin and Tavg lapse rates are more variable and tend to be steepest in spring and shallowest in midsummer. Different synoptic weather types also influence lapse rates, although differences are tenuous. In general, warmer air masses tend to be associated with steeper lapse rates for maximum temperature, and drier air masses have shallower lapse rates for minimum temperature. The largest diurnal range is produced by dry tropical conditions (clear skies, high solar input). Cross-validation results indicate that the commonly used environmental lapse rate [typically assumed to be −0.65°C (100 m)−1] is solely applicable to maximum temperature and often grossly overestimates Tmin and Tavg lapse rates. Regional lapse rates perform better than the environmental lapse rate for Tmin and Tavg, although for some months rates can be predicted more accurately by using monthly lapse rates. Lapse rates computed for different months, synoptic types, and seasonal–synoptic categories all perform similarly. Therefore, the use of monthly lapse rates is recommended as a practical combination of effective performance and ease of implementation.
Model Calculations of Solar Spectral Irradiance in the 3.7-μm Band for Earth Remote Sensing Applications
Authors: Platnick Steven , Fontenla Juan M. 
Published: February 2008

Abstract
Since the launch of the first Advanced Very High Resolution Radiometer (AVHRR) instrument aboard the Television and Infrared Observational Satellite (TIROS-N), measurements in the 3.7-μm atmospheric window have been exploited for use in cloud detection and screening, cloud thermodynamic phase and surface snow/ice discrimination, and quantitative cloud particle size retrievals. The utility of the band has led to the incorporation of similar channels on a number of existing satellite imagers and future operational imagers. Daytime observations in the band include both reflected solar and thermal emission energy. Since 3.7-μm channels are calibrated to a radiance scale (via onboard blackbodies), knowledge of the top-of-atmosphere solar irradiance in the spectral region is required to infer reflectance. Despite the ubiquity of 3.7-μm channels, absolute solar spectral irradiance data come from either a single measurement campaign (Thekaekara et al.) or synthetic spectra. In the current study, the historical 3.7-μm band spectral irradiance datasets are compared with the recent semiempirical solar model of the quiet sun by Fontenla et al. The model has expected uncertainties of about 2% in the 3.7-μm spectral region. The channel-averaged spectral irradiances using the observations reported by Thekaekara et al. are found to be 3.2%–4.1% greater than those derived from the Fontenla et al. model for Moderate Resolution Imaging Spectroradiometer (MODIS) and AVHRR instrument bandpasses; the Kurucz spectrum, as included in the Moderate Spectral Resolution Atmospheric Transmittance (MODTRAN4) distribution, gives channel-averaged irradiances 1.2%–1.5% smaller than the Fontenla model. For the MODIS instrument, these solar irradiance uncertainties result in cloud microphysical retrieval uncertainties that are comparable to other fundamental reflectance error sources.
Forcing Mechanisms for Washoe Zephyr—A Daytime Downslope Wind System in the Lee of the Sierra Nevada
Authors: Zhong Shiyuan , Li Ju , Clements Craig B. , De Wekker Stephan F. J. , Bian Xindi 
Published: February 2008

AbstractThis paper investigates the formation mechanisms for a local wind phenomenon known as Washoe Zephyr that occurs frequently in the lee of the Sierra Nevada. Unlike the typical thermally driven slope flows with upslope wind during daytime and downslope at night, the Washoe Zephyr winds blow down the lee slopes of the Sierra Nevada in the afternoon against the local pressure gradient. Long-term hourly surface wind data from several stations on the eastern slope of the Sierra Nevada and rawinsonde sounding data in the region are analyzed and numerical simulations are performed to test the suggested hypotheses on the formation mechanisms for this interesting phenomenon. The results from surface and upper-air climate data analyses and numerical modeling indicate that the Washoe Zephyr is primarily a result of a regional-scale pressure gradient that develops because of asymmetric heating of the atmosphere between the western side of the Sierra Nevada and the elevated, semiarid central Nevada and Great Basin on the eastern side of the Sierra Nevada. The frequent influence of the Pacific high on California in the summer season helps to enhance this pressure gradient and therefore strengthen the flow. Westerly synoptic-scale winds over the Sierra Nevada and the associated downward momentum transfer are not necessary for its development, but strong westerly winds aloft work in concert with the regional-scale pressure gradient to produce the strongest Washoe Zephyr events.