bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical outcomes and complications of tracheal resection and anastomosis in dogs and cats: 20 cases (2009–2022)
Authors: Gaudio E. , Lipscomb V. J. , Cantatore M. , Blacklock K. , Gosling M. , Jack M. , Charlesworth T. , Shales C. J. 
Published: February 2025

ObjectivesTo report clinical presentation, short‐term outcome and complications of tracheal resection and anastomosis in dogs and cats.Materials and MethodsClinical records of pets treated with tracheal resection and anastomosis in seven UK‐based veterinary referral centres were retrospectively reviewed (2009 to 2022).ResultsTwenty client‐owned pets (eight dogs; 12 cats) were included. Clinical signs comprised dyspnoea (n = 15), stridor (n = 5), subcutaneous emphysema (n = 3), pneumothorax and pneumomediastinum (n = 1), coughing (n = 3), regurgitation (n = 1) and exercise intolerance (n = 1). Indication for surgery was traumatic tracheal rupture (n = 5), tracheal avulsion (n = 7), neoplasia (n = 5) and stenosis (n = 3). Complications occurred in 15 patients (75%). Non‐anastomotic complications occurred in nine patients and consisted of cough (n = 5), self‐resolving laryngeal paralysis (n = 1), surgical site infection (n = 1) and septic shock (n = 2). Anastomotic complications occurred in seven patients and consisted of anastomotic dehiscence (n = 1), tracheal stenosis (n = 4) and fatal respiratory failure (n = 2). Revision surgery was performed in two dogs due to anastomotic dehiscence and stenosis, and tracheal stenting was performed in one cat due to stenosis. Sixteen patients survived to hospital discharge and 14 survived to latest follow‐up (median: 119 days; range: 14 to 1744). Outcome was scored as excellent in five, good in seven, fair in two and poor in six patients.Clinical SignificanceTracheal resection and anastomosis is the surgical technique of choice to reconstruct tracheal defects. Although more than half of the patients recovered well, high morbidity and mortality rates were documented. This may be due to the severe comorbidities affecting most tracheal resection and anastomosis patients, as well as the client's decision against further treatment when facing serious post‐operative complications.
The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum–Plasma Membrane Contact Sites and Interacts With AVRPM3b2/c2 Through Its LRR Domain
Authors: Isaksson Jonatan , Kunz Lukas , Flückiger Simon , Widrig Victoria , Keller Beat 
Published: February 2025

ABSTRACTPlant nucleotide‐binding leucine‐rich repeat (NLR) proteins are intracellular immune receptors that directly or indirectly perceive pathogen‐derived effector proteins to induce an immune response. NLRs display diverse subcellular localisations, which are associated with the capacity of the immune receptor to confer disease resistance and recognise its corresponding avirulence effector. In wheat, the NLR PM3b recognises the wheat powdery mildew effector AVRPM3b2/c2 and we examined the molecular mechanism underlying this recognition. We show that PM3b and other PM3 variants localise to endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (EPCS), while AVRPM3b2/c2 localises to the nucleocytoplasmic space. Additionally, we found that PM3b interacts in planta with AVRPM3b2/c2 through its LRR domain. We further demonstrate that full‐length PM3b interaction with AVRPM3b2/c2 is considerably weaker than for the isolated PM3b LRR domain or the susceptible PM3 variant PM3CS, indicating that activation of PM3b leads to dissociation of the complex. In line with this, we observed a strong interaction between PM3b and AVRPM3b2/c2 in a P‐loop mutant of PM3b that was unable to initiate a cell death response, or when an inactive variant of AVRPM3b2/c2 was used. We propose that PM3b transiently interacts with AVRPM3b2/c2 through residues in the LRR that are conserved among PM3 variants, while the amino acids necessary for full activation and cell death signalling are unique to PM3b. Our data suggests that PM3b localisation and interaction with AVRPM3b2/c2 differ from other well‐studied NLRs and further highlights the mechanistic diversity in NLR‐mediated responses against pathogens in plants.
Sedation in Home Care Surgical Procedures for a Patient With Dental Phobia: A Case Report
Authors: Oliveira Victoria Geisa Brito de , Ghelardi Isis Raquel , Ichimura Karina Tamie , Reinato Isadora Maria de Souza , Jardini Maria Aparecida Neves , Lopes Sérgio Lucio Pereira de Castro , Mendes Mariana Sarmet Smiderle 
Published: February 2025

ABSTRACTAn 88‐year‐old woman received dental evaluation at home after missing out on regular dental checks due to a stroke 3 years ago. Her orofacial complaints included pain in the right side of the face and a desire to use dental prosthetics. Her comorbidities included systemic arterial hypertension and sequels from episodes of a thrombotic stroke and an acute myocardial infarction. Medications used were a beta‐blocker, an angiotensin‐converting enzyme inhibitor, a statin, and an antiplatelet agent. During the initial evaluation, the patient was alert, using a wheelchair, and was quite anxious and apprehensive, reporting episodes of dental phobia. On intraoral examination, findings consistent with the orofacial pain mentioned by the patient were observed. Sequentially, a treatment plan was developed to address the oral condition. The treatment was initiated with basic periodontal therapy and restorative procedures, performed with non‐pharmacological stress reduction management. However, due to the patient's behavior during previous follow‐ups, it was decided to perform extractions under sedation with antihistamine and nitrous oxide in the home setting. The procedure was conducted with vital signs monitoring, and the use of antiplatelet drugs was not suspended. Local bleeding control measures were applied, and the postoperative period occurred without complications. Besides promoting accessibility, home dental care provides treatment in a safe environment for the patient, which enhances comfort and reduces patient anxiety. Additionally, using sedation with antihistamine and nitrous oxide achieved an adequate level of relaxation for more effective stress control during the extractions.
One Size Fits All?—The Form and Function of Preschoolers’ Strategy Flexibility in Emotion Regulation
Authors: Lüken Luisa Marie , Silkenbeumer Judith Rebecca , Holodynski Manfred , Kärtner Joscha 
Published: February 2025

ABSTRACTEffective emotion regulation is critical for establishing and maintaining positive relationships, and it has previously been linked to several indicators of social competence. Theories agree that one core characteristic of adaptive emotion regulation is the ability to flexibly adapt emotion regulation strategies to situational demands (i.e., strategy flexibility). However, little is known about the development of this ability and its association with social competence at preschool age. We hypothesized a positive association of preschoolers’ strategy flexibility with age, with overall regulation effectiveness as well as with different indicators of their social competence. To test these assumptions, we examined the emotion regulation strategies (i.e., distraction, gaze aversion, reappraisal, self‐instruction, and self‐soothing) of 60 preschoolers (M = 59.67 months; SD = 8.18; 45% girls) in two standardized situations, namely a frustration‐eliciting persistence task and a challenging waiting situation, in which attentional deployment strategies have opposing effects, requiring flexible strategy use across situations. Children's social competencies in interaction with caregivers, peers, and task demands were assessed through teacher reports. Results reveal that the effectiveness of attentional deployment strategies changed as predicted. Furthermore, preschoolers’ ability to adapt their emotion regulation strategies to the corresponding task demands was positively associated with age and overall regulation effectiveness. Concerning teacher‐reported facets of children's social competence, strategy flexibility was significantly associated with children's integration into their peer group.
CRF12 specifically regulates the flowering time of Arabidopsis thaliana under non‐inductive conditions
Authors: Li Xia , Fang Siyu , Chen Wanqin , Liu Siyuan , Zhao Lirong , Xu Zhiyu , Chen Shidie , Liu Yunwei , Du Yang , Deng Luyao , Liu Lei , Wang Ting , Li Pingping , Zhu Yi , Yu Diqiu , Wang Houping 
Published: February 2025

SUMMARYThe flowering time of Arabidopsis thaliana, a model plant, is significantly accelerated when exposed to long‐day (LD) conditions, as it is a typical LD plant. Consequently, the investigation of the flowering regulatory network in A. thaliana under LD conditions has garnered considerable attention in the study of flowering signals, resulting in a significant breakthrough. While many LD plants, including A. thaliana, exhibit delayed flowering under non‐inductive short‐day (SD) conditions, they are still capable of flowering. Nevertheless, research on the regulation of flowering induction in LD plants under non‐inductive SD conditions has been limited. This study demonstrated the involvement of CYTOKININ RESPONSE FACTORS 12 (CRF12) in the regulation of flowering in A. thaliana under non‐inductive conditions. Analysis of the expression patterns revealed that the activation of CRF12 expression and protein stability occurred exclusively in non‐inductive environments. Molecular and genetic analyses revealed that under a non‐inductive photoperiod of 12 h of light and 12 h of darkness, CRF12, CONSTANS (CO), and TARGET OF EAT 1/2 (TOE1/2) engage in competitive interactions to regulate flowering time, while in a SD photoperiod of 8 h of light and 16 h of darkness, CRF12 modulates flowering time by inhibiting the activity of TOE1/2.
Water footprint concept, approaches, and applications: A comprehensive review for the agricultural sector
Authors: Demir Muhammed Sungur , Muratoglu Abdullah 
Published: February 2025

AbstractWater footprint (WF) assessment has emerged as a vital tool for understanding and managing agricultural water consumption amid increasing water scarcity. This review synthesizes current knowledge and methodological developments in agricultural WF research, examining both volumetric and Life Cycle Assessment approaches. We analyze key methodological challenges, including water component differentiation, effective precipitation estimation, and calculation standardization across spatial scales. Recent trends show a shift from global assessments toward regional analyses, enabling precise water management strategies. The review evaluates WF reduction methods, demonstrating that optimized crop patterns and improved agricultural practices can significantly reduce water consumption. Integration of hydrological models with WF assessments shows promise for improving accuracy. Climate change, with varying regional impacts, emerges as a critical factor influencing future WF calculations. While WF methodology offers valuable insights for sustainable water management, its practical application requires careful consideration of regional contexts and limitations.
Understanding the Effect of a Changing Climate on the Re‐Emergence of Mosquito‐Borne Diseases in Vulnerable Small Island Nations: A Systematic Review
Authors: Teeluck Mohabeer , Adegboye Oyelola , Karl Stephan , Iyaloo Diana P. , McBryde Emma 
Published: February 2025

ABSTRACTIntroductionDrastic changes in meteorological variables due to climate change will likely have an implication on the proliferation of vectors such as mosquitoes. Extreme weather events may therefore promote the emergence/re‐emergence of mosquito‐borne diseases (MBDs) and potentiate the risk of endemicity, particularly, in small island nations.MethodA systematic review was chosen to methodically ascertain the knowledge gaps that exist in determining the influence of the changing climate on MBDs in small islands with vulnerable public health systems. This review was conducted using the PRISMA guidelines.ResultsFollowing extraction of 600 articles from the databases, 16 studies were determined to meet the selection criteria. The majority of these research papers were from Sri Lanka (n = 9) while the remaining articles were distributed between islands in the Pacific and Atlantic Ocean. Several of these studies used regression modelling techniques to discuss the effect of multiple meteorological variables on the incidence of MBDs. A positive relationship was observed between temperature and the relative risk of MBDs in 72% of the papers. Rainfall enhanced dengue transmission in 84% of the studies included. All the articles discussing the effect of humidity illustrated a similar trend while wind speed was the only climatic variable demonstrating a negative relationship with MBDs.DiscussionConsidering the intricate nature of the non‐linear exposure–response link is crucial when estimating the lagged effect of the changing climate on MBDs transmission. Other challenges associated with bias and confounders in the selected studies as well as meteorological data accessibility, were highlighted. Therefore, it was not possible to conclusively establish that the changing climatic variables do influence the spread of MBDs which accentuated the need for conducting further studies to illustrate the effect of changing weather variables on the incidence of MBDs, with an emphasis on vulnerable small island nations.
Head form variation in mountain newts of the genus Calotriton (Amphibia, Salamandridae) in a phylogeographic context
Authors: García‐Salmerón Alejandro , Muñoz‐Muñoz Francesc , Soler‐Membrives Anna , Valbuena‐Ureña Emilio 
Published: February 2025

AbstractRelief has a profound impact on the genetic structure of species. While the geographic ordination of genetic variation is assessed in many mountain species, the factors involved in phenotypic differentiation and its congruence with phylogeographic history have been largely underexplored. This is the case of the genus Calotriton. Here we use a protocol that allows the study, through geometric morphometrics, of the shape of the head of live animals in situ with the aim of obtaining information on the factors involved in morphological differentiation. To this end, we assess the general pattern of head shape variation in the two species of the genus Calotriton and evaluate its congruence with genetic and geographic structures. The results indicate that differences in head size and shape occur both between and within species. The degree of differentiation between species is congruent with the absence of hybridization and the precise pattern of change suggests that differences in head form could be, at least in part, adaptive. Sexual dimorphism in head size and shape was similar in both species, with males having bigger and more robust heads than females. Geographic variation in head shape and size was congruent with the phylogeographic pattern, but not with divergence times, especially within C. asper. This, together with the high divergence detected between some close populations and the convergence of distant populations, suggests that adaptation to local environments, as well as plastic and/or stochastic factors, are occurring. Our results let us to conclude that the procedure here employed is suitable for detecting head form variation at inter and intraspecific levels.
Turning over new ideas in human skeletal muscle proteostasis: What do we know and where to from here?
Authors: Lim Changhyun , McKendry James , Lees Matthew , Atherton Philip J. , Burd Nicholas A. , Holwerda Andrew M. , van Loon Luc J. C. , McGlory Chris , Mitchell Cameron J. , Smith Kenneth , Wilkinson Daniel J. , Stokes Tanner , Phillips Stuart M. 
Published: February 2025

AbstractUnderstanding the turnover of proteins in tissues gives information as to how external stimuli result in phenotypic change. Nowhere is such phenotypic change more conspicuous than skeletal muscle, which can be effectively remodelled by increased loading, ageing and unloading (disuse), all of which are subject to modification by nutrition and other environmental stimuli. The understanding of muscle proteome remodelling has undergone a renaissance recently with the reintroduction of deuterated water (D2O) and its ingestion to label amino acids and measure their incorporation into proteins. However, there is confusion around the use of the deuterated water methodology and the interpretation of the data it provides. Here, we provide a short review of some of the more salient features of the method and clarify some of the confusion around the method of deuterated water methods and its use in humans and how the interpretation of the data is in contrast to that of rodents.
Model-informed dose optimization for prophylactic piperacillin-tazobactam in perioperative pediatric critically ill patients
Authors: Tan Wen Rui , Irie Kei , McIntire Carter , Torres Julie Luna , Jones Rhonda , Gibson Abigayle , Mizuno Tomoyuki , Tang Girdwood Sonya 
Published: February 2025

ABSTRACT

Piperacillin/tazobactam (PTZ) is frequently prescribed during the perioperative period as prophylaxis in critically ill patients. Current international guidelines recommend that the pediatric intraoperative dosing regimen for PTZ be 90–112.5 mg/kg (80–100 mg/kg as piperacillin [PIP]) administered every 2 hours (Q2H). Concerns have been raised not only about the risk of nephrotoxicity due to elevated PIP exposure but also regarding the practicality of adhering to a 2-h dosing interval in clinical settings. To address these concerns, we employed population pharmacokinetic (PK) modeling and simulation approaches to optimize PTZ dosing regimens in pediatric intraoperative patients. PIP plasma concentration data were obtained from 34 patients using an opportunistic sampling strategy. A two-compartment model was found to adequately describe the PK data. Creatinine clearance was identified as a significant covariate on clearance. The inclusion of inter-occasion variability significantly improved model fit. Simulations across body weights of 10–70 kg and creatinine clearance of 20–130 mL/min/1.73 m
2
demonstrated that 6–15 mg/kg Q2H, or a 10 mg/kg loading dose followed by 1.0–2.75 mg/kg/h continuous infusion would achieve free PIP concentrations being above the minimum inhibitory concentration (MIC) for 100% of the dosing interval (100%
f
T >1× MIC). For achieving 100%
f
T >4× MIC, 25–55 mg/kg Q2H or a 20 mg/kg loading dose followed by 3.25–9.25 mg/kg/h continuous infusion was derived. The model-informed simulations indicated that both lower Q2H doses and continuous infusion methods are clinically viable options and potentially resolve current clinical challenges during intraoperative dosing.
Bumble bee gut microbial community structure differs between species and commercial suppliers, but metabolic potential remains largely consistent
Authors: Hotchkiss Michelle Z. , Poulain Alexandre J. , Forrest Jessica R. K. 
Published: February 2025

ABSTRACT

Bumble bees are key pollinators for natural and agricultural plant communities. Their health and performance are supported by a core gut microbiota composed of a few bacterial taxa. However, the taxonomic composition and community structure of bumble bee gut microbiotas can vary with bee species, environment, and origin (i.e., whether colonies come from the wild or a commercial rearing facility), and it is unclear whether metabolic capabilities therefore vary as well. Here we used metagenomic sequencing to examine gut microbiota community composition, structure, and metabolic potential across bumble bees from two different commercial
Bombus impatiens
suppliers, wild
B. impatiens
, and three other wild bumble bee species sampled from sites within the native range of all four species. We found that the community structure of gut microbiotas varied between bumble bee species, between populations from different origins within species, and between commercial suppliers. Notably, we found that
Apibacter
is consistently present in some wild bumble bee species—suggesting it may be a previously unrecognized core phylotype of bumble bees—and that commercial
B. impatiens
colonies can lack core phylotypes consistently found in wild populations. However, despite variation in community structure, the high-level metabolic potential of gut microbiotas was largely consistent across all hosts, including for metabolic capabilities related to host performance, though metabolic activity remains to be investigated.

IMPORTANCE
Our study is the first to compare genome-level taxonomic structure and metabolic potential of whole bumble bee gut microbiotas between commercial suppliers and between commercial and wild populations. In addition, we profiled the full gut microbiotas of three wild bumble bee species for the first time. Overall, our results provide new insight into bumble bee gut microbiota community structure and function and will help researchers evaluate how well studies conducted in one bumble bee population will translate to other populations and species. Research on taxonomic and metabolic variation in bumble bee gut microbiotas across species and origins is of increasing relevance as we continue to discover new ways that social bee gut microbiotas influence host health, and as some bumble bee species decline in range and abundance.