bluuebunny/crossref_metadata_2025_split huggingface dataset:
An urban Italian study on emergency room utilisation by immigrants suffering from mental disorders in Rome, 2000–2004
Authors: Gaddini A. , Franco F. , Biscaglia L. , Di Lallo D. 
Published: January 2008

AbstractPurposeTo compare Emergency Room (ER) utilisation by subjects diagnosed as suffering from a mental disorder, who were born in Strong Migratory Pressure Countries (SMPC) or in Italy. To evaluate the predictors of admission to psychiatric and to non-psychiatric wards.MethodData collected from Information System of Emergency Rooms were analysed. ER contacts in the years 2000–2004 pertaining to subjects who received a psychiatric diagnosis (ICD9-CM codes), and who were born in SMPC or in Italy were examined. “Contacts” included a total of 68,867 assessments made in the ER of all general hospitals in Rome having an acute psychiatric ward. Gender, age and clinical information on SMPC-born and Italian-born patients were compared. A multinomial logistic regression analysis was performed in order to determine risk factors for admission to a psychiatric or to a non-psychiatric ward.ResultsAt the end of follow-up, 11.7% of ER contacts concerned patients born in SMPC. Compared to the Italian-born group, these patients were younger and received more frequently a diagnosis of “Alcohol and substance abuse and dependencies”, while admissions to a psychiatric ward were significantly less common.ConclusionMonitoring health service utilisation may provide relevant information for the delivery of culturally sensitive mental health services.
Rett syndrome in adults with severe intellectual disability: Exploration of behavioral characteristics
Authors: Matson Johnny L. , Dempsey Timothy , Wilkins Jonathan 
Published: January 2008

AbstractRett syndrome is a genetically linked form of autism spectrum disorder (ASD) accompanied by intellectual disability (ID). The disorder is also characterized by cardiorespiratory dysregulation, disturbance in muscle tone, reduced brain growth and scoliosis. Over 300 studies have been published on the disorder, most of which has focused on identification of causative factors, which appears to be the result of mutations of gene MECP2. Rarely have adults with Rett syndrome been studied, and behavioral characteristics in these individuals are largely unknown. The present study aimed to extend what little is known about behavioral characteristics of Rett syndrome in adults, with particular emphasis on social, communicative, and adaptive behavior. Rett syndrome adults with severe ID were matched to autistic adults with ID and ID only controls. The implications of these data for more fully describing and diagnosing the condition in adults are discussed.
Effectiveness of cryotherapy treatment for cervical intraepithelial neoplasia
Authors: Luciani Silvana , Gonzales Miguel , Munoz Sergio , Jeronimo Jose , Robles Sylvia 
Published: January 2008

AbstractObjectiveTo assess the effectiveness of cryotherapy treatment delivered by general practitioners in primary care settings, as part of a screen‐and‐treat approach for cervical cancer prevention.MethodWomen aged between 25 and 49 years residing in San Martin, Peru, who were positive on visual inspection screening were treated, if eligible, with cryotherapy following biopsy. At 12 months post cryotherapy treatment the participants were evaluated for treatment effectiveness and examined by visual inspection and Papanicolaou test and, if positive, referred to a gynecologist for colposcopy and biopsy.ResultsCryotherapy treatment was performed for 1398 women; of these, 531 (38%) had a histology result of cervical intraepithelial neoplasia (CIN). Cryotherapy effectively cured CIN in 418 (88%) women, including 49 (70%) women with a baseline diagnosis of CIN 3.ConclusionCryotherapy is an effective treatment for cervical precancerous lesions; it can easily be administered by general practitioners in primary care settings following visual inspection screening.
Arctic shipping guidelines: towards a legal regime for navigation safety and environmental protection?
Authors: Jensen Øystein 
Published: January 2008

ABSTRACTWith the International Polar Year (IPY) having commenced in March 2007, key issues relating to the polar regions are again in focus. This article reviews one central legal issue re-emerging in the Arctic: global regulation of safety standards for international shipping. The ‘Guidelines for ships operating in Arctic ice-covered waters’ are examined, with a view to the probable expansion of shipping in the Arctic in near future. Following an introduction to navigational issues within the Arctic context, the article describes how the guidelines came into being, and then analyses key elements and structure of the regulations and shortfalls of today's arrangements. The possible relevance of the guidelines to the Antarctic is also discussed briefly. Finally, the article inquires into the key repercussions of introducing binding regulations.
The United States 2002 Unified Command Plan: Antarctica and the areas of responsibility of military commanders
Authors: Dodds Klaus , Hemmings Alan D. 
Published: January 2008

ABSTRACTIn October 2002, following the 11 September 2001 attacks on the United States, the Unified Command Plan (UCP), that sets out the geographical responsibilities of combatant commanders, was revised with regard to their areas of responsibility. Accompanying these changes was a map, which detailed the geographical boundaries of the US Northern, Pacific, Southern, European and Central Commands. The map indicated that two of these Commands, Southern and European Command stretched as far south as the Antarctic coastline while a third (Pacific) not only did that but also included the entire Antarctic continent. In 2007, a new Africa Command comprising the southern part of European Command, was instituted and this, too, stretched to the Antarctic coastline. This note briefly considers some of the implications that might follow from these changes to the UCP and highlights logistical patterns, search and rescue matters and the question of the demilitarisation of Antarctica.
Methodology for calculating a country's need for positron emission tomography scanners
Authors: Cleemput Irina , Camberlin Cécile , Van den Bruel Ann , Ramaekers Dirk 
Published: January 2008

Objectives:The aim of this study was to develop a methodology for calculating the need for positron emission tomography (PET) scanners in a country and illustrate this methodology for Belgium.Methods:First, levels of evidence were assigned to PET in different indications according to a standard hierarchical classification system. The level reached depends on whether there is evidence on diagnostic accuracy, impact on diagnostic thinking, therapeutic impact, impact on patient outcomes, or cost-effectiveness. Second, the number of patients eligible for PET for each indication was derived from a registry of PET. Third, the number of PET scanners needed in Belgium was estimated for different baseline hypotheses about maximum annual capacity of a scanner and the minimally required level of evidence.Results:The number of PET scanners needed crucially depends on the level of evidence considered acceptable for the implementation of PET: the higher the level of evidence required, the lower the number of PET scanners needed. Belgium needs at least three and at most ten PET scanners. This contrasts with the thirteen currently approved.Conclusions:Scientific evidence and information on the eligible population for a specific procedure are crucial elements for policy makers who wish to make evidence-based decisions about programming and planning of heavy medical equipment.
Systematic review of the impact of endoscopic ultrasound on the management of patients with esophageal cancer
Authors: Dyer Suzanne M. , Levison Dane B. , Chen Robert Y. , Lord Sarah J. , Blamey Stephen 
Published: January 2008

Objectives:Although endoscopic ultrasound (EUS) staging of esophageal cancer is established in clinical practice, high-quality evidence about its impact on patient outcomes is not available. This study aims to determine the impact of EUS for esophageal cancer staging on patient management and survival.Methods:A systematic review was conducted using Medline, PreMedline, Embase, and The Cochrane Library. Included studies were (i) comparative studies reporting survival following EUS esophageal cancer staging, (ii) therapeutic impact studies reporting change in patient management following EUS. The quality of included studies was critically appraised.Results:One systematic review, five studies reporting therapeutic impact, and two studies reporting patient survival were identified. The design and quality of the therapeutic impact studies varied widely. Management changed in 24–29 percent of patients following EUS staging of esophageal cancer (two studies). No studies provided data on the avoidance of surgery for this indication. One retrospective cohort study with historical control found EUS staging of esophageal cancer improved patient survival; a second study with similar design limitations did not find a survival benefit for EUS staging in patients undergoing resection. These studies had a high potential for bias, limiting the value of these findings.Conclusions:Two studies provided evidence of a change in patient management following EUS for staging esophageal cancer, a higher level of evidence for a clinical benefit than can be obtained from accuracy studies alone. This evidence contributed to a recommendation for public funding of EUS in staging esophageal cancer in Australia.
Cost-effectiveness analysis of Helicobacter pylori screening in prevention of gastric cancer in Chinese
Authors: Xie Feng , Luo Nan , Blackhouse Gord , Goeree Ron , Lee Hin-Peng 
Published: January 2008

Objectives:The aim of this study was to evaluate the costs and effectiveness associated with no screening,Helicobacter pyloriserology screening, and the13C-urea breath test (UBT) for gastric cancer in the Chinese population.Methods:A Markov model simulation was carried out in Singaporean Chinese at 40 years of age (n= 478,500) from the perspective of public healthcare providers. The main outcome measures were costs, number of gastric cancer cases prevented, life-years saved, quality-adjusted life-years (QALYs) gained from the screening age to death, and incremental cost-effectiveness ratios (ICERs), which were compared among the three strategies. The uncertainty surrounding ICERs was addressed by scenario analyses and probabilistic sensitivity analysis using Monte Carlo simulation.Results: The ICER of serology screening versus no screening was $25,881 per QALY gained (95 percent confidence interval (95 percent CI), $5,700 to $120,000). The ICER of UBT versus no screening was $53,602 per QALY gained (95 percent CI, $16,000 to $230,000). ICER of UBT versus serology screening was $470,000 per QALY gained, for which almost all random samples of the ICERs distributed above $50,000 per QALY.Conclusions: It cannot be confidently concluded that eitherH pyloriscreening was a cost-effective strategy compared with no screening in all Chinese at the age of 40 years. Nevertheless, serology screening has demonstrated much more potential to be a cost-effective strategy, especially in the population with higher gastric cancer prevalence.
Pasteurella multocidaand bovine respiratory disease
Authors: Dabo S. M. , Taylor J. D. , Confer A. W. 
Published: January 2008

AbstractPasteurella multocidais a pathogenic Gram-negative bacterium that has been classified into three subspecies, five capsular serogroups and 16 serotypes.P. multocidaserogroup A isolates are bovine nasopharyngeal commensals, bovine pathogens and common isolates from bovine respiratory disease (BRD), both enzootic calf pneumonia of young dairy calves and shipping fever of weaned, stressed beef cattle.P. multocidaA:3 is the most common serotype isolated from BRD, and these isolates have limited heterogeneity based on outer membrane protein (OMP) profiles and ribotyping. Development ofP. multocida-induced pneumonia is associated with environmental and stress factors such as shipping, co-mingling, and overcrowding as well as concurrent or predisposing viral or bacterial infections. Lung lesions consist of an acute to subacute bronchopneumonia that may or may not have an associated pleuritis. Numerous virulence or potential virulence factors have been described for bovine respiratory isolates including adherence and colonization factors, iron-regulated and acquisition proteins, extracellular enzymes such as neuraminidase, lipopolysaccharide, polysaccharide capsule and a variety of OMPs. Immunity of cattle against respiratory pasteurellosis is poorly understood; however, high serum antibodies to OMPs appear to be important for enhancing resistance to the bacterium. Currently availableP. multocidavaccines for use in cattle are predominately traditional bacterins and a live streptomycin-dependent mutant. The field efficacy of these vaccines is not well documented in the literature.
Comparison of Metabolic Flux Distributions for MDCK Cell Growth in Glutamine‐ and Pyruvate‐Containing Media
Authors: Sidorenko Yury , Wahl Aljoscha , Dauner Michael , Genzel Yvonne , Reichl Udo 
Published: January 2008

AbstractIn mammalian cell cultures, ammonia that is released into the medium as a result of glutamine metabolism and lactate that is excreted due to incomplete glucose oxidation are both known to essentially inhibit the growth of cells. For some cell lines, for example, hybridoma cells, excreted ammonia also has an effect on product formation. Although glutamine has been generally considered as the major energy source for mammalian cells, it was recently found that various adherent cell lines (MDCK, CHO‐K1, and BHK21) can grow as well in glutamine‐free medium, provided glutamine is substituted with pyruvate. In such a medium the level of both ammonia and lactate released was significantly reduced. In this study, metabolic flux analysis (MFA) was applied to Madin Darby Canine Kidney (MDCK) cells cultivated in glutamine‐containing and glutamine‐free medium. The results of the MFA allowed further investigation of the influence of glutamine substitution with pyruvate on the metabolism of MDCK cells during different growth stages of adherent cells, e.g., early exponential and late contact‐inhibited phase. Pyruvate seemed to directly enter the TCA cycle, whereas most of the glucose consumed was excreted as lactate. Although the exact mechanisms are not clear so far, this resulted in a reduction of the glucose uptake necessary for cellular metabolism in glutamine‐free medium. Furthermore, consumption of ATP by futile cycles seemed to be significantly reduced when substituting glutamine with pyruvate. These findings imply that glutamine‐free medium favors a more efficient use of nutrients by cells. However, a number of metabolic fluxes were similar in the two cultivations considered, e.g., most of the amino acid uptake and degradation rates or fluxes through the branch of the TCA cycle converting α‐ketoglutarate to malate, which is responsible for the mitochondrial ATP synthesis. Besides, the specific rate of cell growth was approximately the same in both cultivations. Thus, the switch from glutamine‐containing to glutamine‐free medium with pyruvate provided a series of benefits without dramatic changes of cellular metabolism.
Vaccine To Confer to Nonhuman Primates Complete Protection against Multistrain Ebola and Marburg Virus Infections
Authors: Swenson Dana L. , Wang Danher , Luo Min , Warfield Kelly L. , Woraratanadharm Jan , Holman David H. , Dong John Y. , Pratt William D. 
Published: January 2008

ABSTRACT
Filoviruses (Ebola and Marburg viruses) are among the deadliest viruses known to mankind, with mortality rates nearing 90%. These pathogens are highly infectious through contact with infected body fluids and can be easily aerosolized. Additionally, there are currently no licensed vaccines available to prevent filovirus outbreaks. Their high mortality rates and infectious capabilities when aerosolized and the lack of licensed vaccines available to prevent such infectious make Ebola and Marburg viruses serious bioterrorism threats, placing them both on the category A list of bioterrorism agents. Here we describe a panfilovirus vaccine based on a complex adenovirus (CAdVax) technology that expresses multiple antigens from five different filoviruses de novo. Vaccination of nonhuman primates demonstrated 100% protection against infection by two species of Ebola virus and three Marburg virus subtypes, each administered at 1,000 times the lethal dose. This study indicates the feasibility of vaccination against all current filovirus threats in the event of natural hemorrhagic fever outbreak or biological attack.
NPXY Motifs in the β1 Integrin Cytoplasmic Tail Are Required for Functional Reovirus Entry
Authors: Maginnis Melissa S. , Mainou Bernardo A. , Derdowski Aaron , Johnson Elizabeth M. , Zent Roy , Dermody Terence S. 
Published: January 2008

ABSTRACT
Reovirus cell entry is mediated by attachment to cell surface carbohydrate and junctional adhesion molecule A (JAM-A) and internalization by β1 integrin. The β1 integrin cytoplasmic tail contains two NPXY motifs, which function in recruitment of adaptor proteins and clathrin for endocytosis and serve as sorting signals for internalized cargo. As reovirus infection requires disassembly in the endocytic compartment, we investigated the role of the β1 integrin NPXY motifs in reovirus internalization. In comparison to wild-type cells (β1+/+ cells), reovirus infectivity was significantly reduced in cells expressing mutant β1 integrin in which the NPXY motifs were altered to NPXF (β1+/+Y783F/Y795F cells). However, reovirus displayed equivalent binding and internalization levels following adsorption to β1+/+ cells and β1+/+Y783F/Y795F cells, suggesting that the NPXY motifs are essential for transport of reovirus within the endocytic pathway. Reovirus entry into β1+/+ cells was blocked by chlorpromazine, an inhibitor of clathrin-mediated endocytosis, while entry into β1+/+Y783F/Y795F cells was unaffected. Furthermore, virus was distributed to morphologically distinct endocytic organelles in β1+/+ and β1+/+Y783F/Y795F cells, providing further evidence that the β1 integrin NPXY motifs mediate sorting of reovirus in the endocytic pathway. Thus, NPXY motifs in the β1 integrin cytoplasmic tail are required for functional reovirus entry, which indicates a key role for these sequences in endocytosis of a pathogenic virus.
The Fusion Protein of Respiratory Syncytial Virus Triggers p53-Dependent Apoptosis
Authors: Eckardt-Michel Julia , Lorek Markus , Baxmann Diane , Grunwald Thomas , Keil Günther M. , Zimmer Gert 
Published: January 2008

ABSTRACT
Infection with respiratory syncytial virus (RSV) frequently causes inflammation and obstruction of the small airways, leading to severe pulmonary disease in infants. We show here that the RSV fusion (F) protein, an integral membrane protein of the viral envelope, is a strong elicitor of apoptosis. Inducible expression of F protein in polarized epithelial cells triggered caspase-dependent cell death, resulting in rigorous extrusion of apoptotic cells from the cell monolayer and transient loss of epithelial integrity. A monoclonal antibody directed against F protein inhibited apoptosis and was also effective if administered to A549 lung epithelial cells postinfection. F protein expression in epithelial cells caused phosphorylation of tumor suppressor p53 at serine 15, activation of p53 transcriptional activity, and conformational activation of proapoptotic Bax. Stable expression of dominant-negative p53 or p53 knockdown by RNA interference inhibited the apoptosis of RSV-infected A549 cells. HEp-2 tumor cells with low levels of p53 were not sensitive to RSV-triggered apoptosis. We propose a new model of RSV disease with the F protein as an initiator of epithelial cell shedding, airway obstruction, secondary necrosis, and consequent inflammation. This makes the RSV F protein a key target for the development of effective postinfection therapies.
Early Phosphatidylinositol 3-Kinase/Akt Pathway Activation Limits Poliovirus-Induced JNK-Mediated Cell Death
Authors: Autret Arnaud , Martin-Latil Sandra , Brisac Cynthia , Mousson Laurence , Colbère-Garapin Florence , Blondel Bruno 
Published: January 2008

ABSTRACT
Poliovirus (PV)-induced apoptosis seems to play a major role in tissue injury in the central nervous system (CNS). We have previously shown that this process involves PV-induced Bax-dependent mitochondrial dysfunction mediated by early JNK activation in IMR5 neuroblastoma cells. We showed here that PV simultaneously activates the phosphatidylinositol 3-kinase (PI3K)/Akt survival signaling pathway in these cells, limiting the extent of JNK activation and thereby cell death. JNK inhibition is associated with PI3K-dependent negative regulation of the apoptosis signal-regulating kinase 1, which acts upstream from JNK in PV-infected IMR5 cells. In poliomyelitis, this survival pathway may limit the spread of PV-induced damage in the CNS.
Functional Analysis of Picornavirus 2B Proteins: Effects on Calcium Homeostasis and Intracellular Protein Trafficking
Authors: de Jong Arjan S. , de Mattia Fabrizio , Van Dommelen Michiel M. , Lanke Kjerstin , Melchers Willem J. G. , Willems Peter H. G. M. , van Kuppeveld Frank J. M. 
Published: January 2008

ABSTRACT

The family
Picornaviridae
consists of a large group of plus-strand RNA viruses that share a similar genome organization. The nomenclature of the picornavirus proteins is based on their position in the viral RNA genome but does not necessarily imply a conserved function of proteins of different genera. The enterovirus 2B protein is a small hydrophobic protein that, upon individual expression, is localized to the endoplasmic reticulum (ER) and the Golgi complex, reduces ER and Golgi complex Ca
2+
levels, most likely by forming transmembrane pores, and inhibits protein trafficking through the Golgi complex. At present, little is known about the function of the other picornavirus 2B proteins. Here we show that rhinovirus 2B, which is phylogenetically closely related to enterovirus 2B, shows a similar subcellular localization and function to those of enterovirus 2B. In contrast, 2B proteins of hepatitis A virus, foot-and-mouth disease virus, and encephalomyocarditis virus, all of which are more distantly related to enteroviruses, show a different localization and have little, if any, effects on Ca
2+
homeostasis and intracellular protein trafficking. Our data suggest that the 2B proteins of enterovirus and rhinovirus share the same function in virus replication, while the other picornavirus 2B proteins support the viral life cycle in a different manner. Moreover, we show that an enterovirus 2B protein that is retained in the ER is unable to modify Ca
2+
homeostasis and inhibit protein trafficking, demonstrating the importance of Golgi complex localization for its functioning.