bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glutamine deprivation alters TGF-β signaling in hepatocellular carcinoma
Authors: Gélabert Caroline , Campisano Sabrina , Golán Irene C. , Beyene Nateneal T. , Heldin Carl-Henrik , Chisari Andrea , Sancho Patricia , Moustakas Aristidis , Caja Laia 
Published: March 2024

AbstractMetabolic reprogramming is one of the hallmarks of cancer. Glutamine is one of the most important nutrients that fuels the TCA cycle and therefore takes part in the production of energy. Glutamine is used as starting metabolite for the synthesis of nucleotides, fatty acids and non-essential amino acids. Since nutrients are uptaken from the blood stream, and considering the 3-dimensional state of solid tumors, access of nutrients is highly dependent on the location of individual cells within a tumor, which results in affecting their metabolic activity. This gives rise to two disctincts cell population: the ones that have access to nutrient and the ones that are nutrient-deprived. We studied the effect of the lack of glutamine by creating glutamine-resistent hepatocellular carcinoma cell lines chosen based on their epithelial (Hep3B) or mesenchymal phenotype (SNU-499 and HLF). We found that glutamine deprivation decreased the proliferation rate, clonogenicity and stemness frequency of the three cell lines but in a greater extent of the mesenchymal cells. Transcriptomic analysis performed in HLF cells showed that glutamine deprivation decreased the activation of signaling pathways involved in cell-cell junction, cell-extracellular matrix interactions and decreased the expression of the hallmarks of epithelial-to-mesenchymal transition. We therefore investigated the role of TGFβ, a master regulator of these three processes, by transcriptomic and functional analyses in epithelial (Hep3B) and mesenchymal cells (HLF). We found that the lack of glutamine strongly impared the activation of TGFβ signaling which correlated with an altered regulation of TGFβ target genes: the expression of mesenchymal genes was no longer induced by TGFβ while the epithelial genes were more strongly induced. Functional analyses showed that glutamine deprivation abolished the invasive capacities of HCCs and decreased cell adhesion. Altogehter, our results show that glutamine metabolism is necessary to maintain a mesenchymal phenotype and to maintain an efficient TGFβ signaling in hepatocellularcarcinoma.
High resolution mapping of novel non-transgressive hybrid susceptibility in barley exploited byP. teresf.maculatamaps to a single pentatricopeptide repeat-containing protein
Authors: Clare Shaun J. , Alhashel Abdullah F. , Li Mengyuan , Effertz Karl M. , Poudel Roshan Sharma , Zhang Jianwei , Brueggeman Robert S. 
Published: March 2024

AbstractHybrid genotypes can provide significant yield gains over conventional inbred varieties due to heterosis or hybrid vigor. However, hybrids can also display unintended negative attributes or phenotypes such as extreme pathogen susceptibility. The necrotrophic pathogenPyrenophora teresf.maculata(Ptm) causes spot form net blotch, which has caused significant losses to barley worldwide. Here, we report on a non-transgressive hybrid susceptibility locus in barley initially recognized because the three parental lines CI5791, Tifang and Golden Promise are resistant toPtmisolate 13IM.3, however F2progeny from CI5791 × Tifang and CI5791 × Golden Promise crosses exhibited extreme susceptibility. The susceptible phenotype segregated in a ratio of 1 resistant:1 susceptible representing a genetic segregation ratio of 1 parental (res):2 heterozygous (sus):1 parental (res) suggesting a single hybrid susceptibility locus. Genetic mapping using a total of 715 CI5791 × Tifang F2individuals (1430 recombinant gametes) and 149 targeted SNPs delimited the hybrid susceptibility locus designatedSusceptibility to Pyrenophora teres 2(Spt2) to an ∼198 kb region on chromosome 5H of the Morex V3 reference assembly. This single locus was independently mapped with 83 CI5791 × Golden Promise F2individuals (166 recombinant gametes) and 180 genome wide SNPs that colocalized to the sameSpt2locus. The CI5791 genome was sequenced using PacBio Continuous Long Read technology and comparative analysis between CI5791 and the publicly available Golden Promise genome assembly determined that the delimited region contained a single high confidenceSpt2candidate gene predicted to encode a pentatricopeptide repeat-containing protein.
Evaluating Paraspinal Muscle Response and Compensation via Musculoskeletal Modeling in Spinal Stenosis Surgeries
Authors: Jones Ryan , Kumaran Yogesh , Padgaonkar Adit , Hoffman Brett , Behrens Kyle , Elgafy Hossein , Tripathi Sudharshan , Goel Vijay K. 
Published: March 2024

AbstractIntroductionLumbar spinal stenosis is a common cause of lower back pain and weakness in elderly patients. The gold standard treatment for this is lumbar laminectomy which involves widespread muscle damage to the multifidus, a complete loss of the posterior tension band which contains the supraspinous and interspinous ligaments. However, in recent years minimally invasive techniques such as bilateral and unilateral laminotomy have become more popular and are showing efficacy in the decompression of spinal stenosis. Due to its minimally invasive approach, the muscle retraction required for laminotomy is less intensive than that required for laminectomy. The overall body of literature on the surgical treatment of spinal stenosis is sparse in its interrogation of the biomechanical outcomes of these techniques and to our knowledge, there are no current publications that incorporate muscle forces.MethodsA previously validated thoracolumbar ribcage finite element (FE) model was used for this study. Three different surgeries, traditional laminectomy, unilateral and bilateral midline sparing approaches at L4-L5 segment were simulated by removing the spinous process, supraspinous, and interspinous ligaments. The segmental range of motion (ROM) for all models were acquired and input into a musculoskeletal modelling software to calculate muscle forces.ResultsUnilateral and bilateral laminotomy showed similar muscle forces for every muscle group in both flexion and extension motion. While comparing the muscle forces in laminotomy to the laminectomy in extension motion displayed an increase in Iliocostalis lumborum (IL) by 12 % and multifidus (MF) by 16% and decrease in transverse abdominus (TA) by 138% and erector spine (ES) by 12%. For flexion, there was an increase in IL by 35%, and MF by 12%.ConclusionOur results highlight that laminectomy, which involves the removal of paraspinal muscles and posterior ligamentous structures to relieve stenosis, can lead to increased instability and necessitate muscle compensation, particularly in adjacent and thoracic spine segments. Conversely, midline sparing approaches such as laminotomies, are associated with decreased muscle compensation across spinal segments and enhanced stability.
Selective enrichment ofMethylococcaceaeversusMethylocystaceaemethanotrophs via control of methane feeding schemes
Authors: Lee Ju Yong , Choi Munjeong , Song Min Joon , Kim Daehyun Daniel , Yun Taeho , Chang Jin , Ho Adrian , Myung Jaewook , Yoon Sukhwan 
Published: March 2024

ABSTRACTMethanotrophs are crucial in keeping environmental CH4emissions in check. However, how different groups of methanotrophs contribute to this important role in different environmental settings remain ambiguous. Here, in a simplified laboratory setting of well-mixed batch reactors fed continuous flow of CH4-containing gas, methanotrophic microbiomes were enriched from paddy soils under six different incubation conditions prepared as combinations of two different CH4mixing ratios (0.5% and 10% v/v) and three supplemented Cu2+concentrations (0, 2, and 10 μM). Monitoring of the temporal community shifts in the reactor microbiomes observed domination ofMethylocystisspp. in all three reactors fed 0.5% v/v, as further supported by the analyses ofpmoCABgenes in the shotgun metagenomes of the single-point samples from the same reactors. Copper deficiency did not select formmoXYZ-possessing methanotrophs. Instead, a cluster ofmbngenes with an abundance accounting for approximately 5% ofMethylocystispopulation was identified, suggesting a comparative ecological importance of methanobactin in Cu-deficient methanotrophy over soluble methane monooxygenases. These findings highlight the importance ofMethylocystisspp. in mitigating emissions from CH4hotspots, e.g., landfills and rice paddies, and suggest the feasibility of directed enrichment/isolation ofMethylocystisspp. for utilization in, for example, methanobactin and polyhydroxybutyrate production.SynopsisThis study reports enrichment of a complex soil microbiota with 0.5% methane resulting in dominance of a specific group of methane-consuming bacteriaMethylocystis, highlighting their ecological significance as CH4sink.
Mitochondria drive microglial NRLP3 inflammasome activation via TSPO
Authors: Singh Aarti , Rigon Manuel , Caldeira Marta Gramaça , Faccenda Danilo , Xia Dong , Lopez-Tremoleda Jordi , Al-Khateeb Zahra Falah Hassan , Guo Tong , Abeti Rosella , Giunti Paola , Campanella Michelangelo 
Published: March 2024

AbstractUncontrolled microglial response is core to neuroinflammatory brain diseases. The correlation between the mitochondrial protein TSPO and inflammation has so far failed to explain whether TSPO positively or negatively regulates microglial function. The recent evidence on the species specificity of TSPO in microglia demands a deeper understanding of the protein biology in these brain-resident macrophages. To this end, we have here enrolled a murine model of microglial cells showing that TSPO is required for the priming of mitochondria to inflammation and a conduit for its escalation. Namely, in response to inflammatory cues TSPO is stabilised on the mitochondria where it binds and sequesters NOD-like receptor (NLR) protein (i), represses the PARK2-mediated mitophagy (ii) and engages the retrograde communication with the nucleus via the accumulation of the Nf-kB to promote the expression of pro-inflammatory genes (iii). Notably, the TSPO sustained inflammatory response drives cellular demise and ultimately leads to excitotoxicity (iv).Our findings advance the current knowledge of TSPO widening the understanding of mitochondria in inflammation and indicating a target for their regulation.
J/ψ
and
ψ(2S)
polarization in proton-proton collisions at energies available at the CERN Large Hadron Collider using PYTHIA8
Authors: Sahoo Bhagyarathi , Sahu Dushmanta , Deb Suman , Singh Captain R. , Sahoo Raghunath 
Published: March 2024

The production mechanisms of charmonium states in both hadronic and heavy-ion collisions hold great significance for investigating the hot and dense QCD matter. Studying charmonium polarization in ultrarelativistic collisions can also provide insights into the underlying production mechanisms. With this motivation, we explore the J/ψ and ψ(2S) polarization in proton+proton collisions at s=7, 8, and 13 TeV using a pQCD-inspired Monte Carlo event generator called 8. This work considers reconstructed quarkonia through their dimuons decay channel in the ALICE forward rapidity acceptance range of 2.5<yμμ<4. Further, we calculate the polarization parameters λθ, λϕ, λθϕ from the polar and azimuthal angular distributions of the dimuons in helicity and Collins-Soper frames. This study presents a comprehensive measurement of the polarization parameters as a function of transverse momentum, charged-particle multiplicity, and rapidity at the LHC energies. Our findings of charmonium polarization are in qualitative agreement with the corresponding experimental data.

Published by the American Physical Society
2024
Systematic studies of
DDKK
and
DD¯KK¯
four-hadron molecules
Authors: Pan Ya-Wen , Liu Ming-Zhu , Lu Jun-Xu , Geng Li-Sheng 
Published: March 2024

Assuming that Ds0*(2317) is a DK molecular state with a binding energy of 45 MeV, we investigate the existence of four-hadron molecules, DDKK and DD¯KK¯, with the Gaussian expansion method. Their binding energies are 138–155 MeV and 123–163 MeV below the mass thresholds of DDKK and DD¯KK¯. The DD¯KK¯ state has a decay width of 74–107 MeV due to the complex KK¯ interaction. Further theoretical studies of and experimental searches for such four-hadron molecules can help deepen the understanding of the nonperturbative strong interaction in a nontrivial way.

Published by the American Physical Society
2024
LHC study of third-generation scalar leptoquarks with machine-learned likelihoods
Authors: Arganda Ernesto , Díaz Daniel A. , Perez Andres D. , Sandá Seoane Rosa M. , Szynkman Alejandro 
Published: March 2024

We study the impact of machine-learning algorithms on LHC searches for leptoquarks in final states with hadronically decaying tau leptons, multiple b-jets, and large missing transverse momentum. Pair production of scalar leptoquarks with decays only into third-generation leptons and quarks is assumed. Thanks to the use of supervised learning tools with unbinned methods to handle the high-dimensional final states, we consider simple selection cuts which would possibly translate into an improvement in the exclusion limits at the 95% confidence level for leptoquark masses with different values of their branching fraction into charged leptons. In particular, for intermediate branching fractions, we expect that the exclusion limits for leptoquark masses extend to ∼1.3  TeV. As a novelty in the implemented unbinned analysis, we include a simplified estimation of some systematic uncertainties with the aim of studying their possible impact on the stability of the results. Finally, we also present the projected sensitivity within this framework at 14 TeV for 300  fb−1 and 3000  fb−1 that extends the upper limits to ∼1.6  TeV and ∼1.8  TeV, respectively.

Published by the American Physical Society
2024
Capillary discharge in the high repetition rate regime
Authors: Sasorov P. , Bagdasarov G. , Bobrova N. , Grittani G. , Molodozhentsev A. , Bulanov S. V. 
Published: March 2024

Plasma discharge in the capillary is used to develop x-ray lasers, waveguides for high power laser pulses, and as active plasma lenses to focus high energy charged particle beams. Capillary discharges in the high repetition rate regime are of interest for applications that require large average values, such as luminosity and/or electric current of laser accelerated particles. In the present paper, we study the capillary discharge in the high repetition rate regime in connection with the ultrashort laser pulse guiding for laser electron acceleration. Using magnetohydrodynamic computer simulations and theoretical scaling, we investigate the filling of the capillary with the gas, the electric discharge development leading to outflow of the plasma from the capillary, and the recovery of gas distribution after the discharge end. In the next cycle, these processes are repeated. As a result, we found the characteristic cycle time, which determines the upper limit on the repetition rate allowed by the capillary parameters. In the case of the capillary discharges used for acceleration of sub-GeV electron beams, e.g., needed for compact free electron lasers, an upper limit on the repetition rate is approximately equal to 10 kHz.

Published by the American Physical Society
2024
Thermodynamics for reduced models of chemical reactions by PEA and QSSA
Authors: Peng Liangrong , Hong Liu 
Published: March 2024

The partial equilibrium approximation (PEA) and the quasi-steady-state approximation (QSSA) are two classical methods for reducing complex macroscopic chemical reactions into simple computable ones. Previous studies have primarily focused on the accuracy of solutions before and after applying model reduction. In this paper, however, we adopt a thermodynamic view and aim to establish a quantitative connection on the essential thermodynamic quantities, such as entropy production rate, free-energy dissipation rate, and entropy flow rate, between the original chemical mass-action equations and the reduced models obtained by PEA or QSSA. Our findings reveal that the reduced models by PEA and QSSA may not preserve the thermodynamic structure of the original full model, particularly when algebraic relations are used instead of differential equations. This is evident in the loss of non-negativity of the free-energy dissipation rate. To validate our results, we apply them to the Michaelis-Menten reactions as a prototype, both analytically and numerically. We anticipate that our study will inspire a reevaluation of the effectiveness of various model reduction or approximation methods from the perspective of nonequilibrium thermodynamics.

Published by the American Physical Society
2024
I will get my job: moderated and mediation model of students' perceived employability in post-pandemic era
Authors: Ngah Abdul Hafaz , Kamarulzaman Nurul Izni , Puteh Saifullizam , Abdullah Nurul Ain Chua , Ariffin Nur Asma , Fei Long 
Published: March 2024

PurposeThe current study investigates the factors influencing graduates’ perceived employability by utilizing the stimulus-organism-response theory, in the post pandemic era.Design/methodology/approachA quantitative approach was employed to examine the hypotheses of the research framework through partial least squares structural equation modelling (PLS-SEM) on the SmartPLS software.FindingsThe result indicates that course structure has a positive effect on students’ grit and community of inquiry (CoI). Also, students’ grit and CoI have a positive relationship with students’ performance, while students’ performance has a positive relationship with perceived employability. Moreover, students’ grit, CoI and students’ performance sequentially mediated course structure and perceived employability, whereas readiness and self-directed learning strengthen the relationship between students’ performance and perceived employability.Originality/valueThe findings will benefit university management, government and potential employers on how confident the student is in the chances of a future career after graduating from a higher institution.
Legal uncertainty of municipal bond issuance: a case study of Indonesia and Vietnam
Authors: Hutahayan Benny , Fadli Mohamad , Amimakmur Satria Amiputra , Dewantara Reka 
Published: March 2024

Purpose
This study aims to analyze the causes and implications of legal uncertainty in the issuance of conventional municipal bonds in Indonesia and to draw lessons from Vietnam’s approach in providing better legal certainty.

Design/methodology/approach
This study adopts a normative legal method with a legislative approach and applies a comparative approach. Data sources involve primary and secondary legal materials from both Indonesia and Vietnam.

Findings
The legal uncertainty is caused by a lack of coherence and consistency in legislation. Based on Vietnam’s experience, Indonesia can gain valuable insights related to providing strong legal certainty for parties involved in issuing or investing through conventional municipal bonds.

Research limitations/implications
This study focuses on the comparative legal analysis of conventional municipal bonds in Indonesia with Vietnam.

Practical implications
This research provides recommendations for the refinement of legislation regarding conventional municipal bonds to the government.

Social implications
This study is related to legal certainty as a strategy to attract investment through municipal bonds and to ensure the municipal bond issuance process is transparent and efficient.

Originality/value
This study provides a comparative perspective on the issuance of municipal bonds in Indonesia, with a special focus on Vietnam, emphasizing the urgency of harmonization in legal regulation and the sustainability of legal certainty.
A corporate social responsibility (CSR)-led competitiveness framework through a bibliometric analysis (2001–2023)
Authors: Kumar Amit , Snehvrat Saurav , Kumari Prerna , Priyadarshani Priyanka , Ray Preyaan 
Published: March 2024

Purpose
Corporate social responsibility (CSR) is viewed as a differentiating strategy that wins over stakeholders’ confidence. Due to the potential strategic and positive effects on businesses, the study of CSR and its relationship to competitiveness has gained relevance. While studies have examined the impact of CSR activities on firm competitiveness, the findings so far remain contradictory. Further research on the underlying processes/mechanisms that explain how CSR contributes to competitiveness remains scarce. Accordingly, this study aims to look into the link between CSR and competitiveness with a focus on Asian business and management studies.

Design/methodology/approach
By using a bibliometric approach, this paper aims to provide a review of the state-of-the-art research on the linkage between CSR and competitiveness in Asian context. The sample for this research included all 538 studies from the period of 2001–2023 in the Scopus database. A bibliometric study included both co-occurrence and co-citation analysis.

Findings
The study’s findings made significant contributions by identifying seven distinct clusters of co-occurrences. Using co-citation, three journals-based co-citation clusters and another three authors-based co-citation clusters are identified. The findings show how processes/mechanisms such as – accountability, multi-stakeholder dialogue/engagement, resource generation, emphasizing sustainable development goals and emerging markets, redefining strategy, cultivating value/vision and CSR leadership – are increasing in importance.

Practical implications
Overall, the authors argue that CSR-led competitiveness is indeed one of the key drivers for improved sustainability performance of a firm.

Originality/value
Based on findings, a conceptual framework has been proposed highlighting different processes and mechanisms that influence the CSR-led competitiveness – outcomes relationship.
Repair part service level differentiation based on holding other parts shortage costs
Authors: Maleyeff John , Xu Jingran 
Published: March 2024

PurposeThe article addresses the optimization of safety stock service levels for parts in a repair kit. The work was undertaken to assist a public transit entity that stores thousands of parts used to repair equipment acquired over many decades. Demand is intermittent, procurement lead times are long, and the total inventory investment is significant.Design/methodology/approachDemand exists for repair kits, and a repair cannot start until all required parts are available. The cost model includes holding cost to carry the part being modeled as well as shortage cost that consists of the holding cost to carry all other repair kit parts for the duration of the part’s lead time. The model combines deterministic and stochastic approaches by assuming a fixed ordering cycle with Poisson demand.FindingsThe results show that optimal service levels vary as a function of repair demand rate, part lead time, and cost of the part as a percentage of the total part cost for the repair kit. Optimal service levels are higher for inexpensive parts and lower for expensive parts, although the precise levels are impacted by repair demand and part lead time.Social implicationsThe proposed model can impact society by improving the operational performance and efficiency of public transit systems, by ensuring that home repair technicians will be prepared for repair tasks, and by reducing the environmental impact of electronic waste consistent with the right-to-repair movement.Originality/valueThe optimization model is unique because (1) it quantifies shortage cost as the cost of unnecessary holding other parts in the repair kit during the shortage time, and (2) it determines a unique service level for each part in a repair kit bases on its lead time, its unit cost, and the total cost of all parts in the repair kit. Results will be counter-intuitive for many inventory managers who would assume that more critical parts should have higher service levels.