bluuebunny/crossref_metadata_2025_split huggingface dataset:
The first in vitro evaluation of acaricide paeonol against human Demodex (Acari: Demodicidae)
Authors: Xu Yueye , Xu jingang , Shuai Yujun , Teng Qiao , Tu Huanxin , Ren Zhili , Chang Qingquan , Guo Junjie , Li Yuanyuan , Zhao Jinhong 
Published: March 2024

Abstract

Background
Human Demodex (classified into Demodex folliculorum and Demodex brevis) causes multiple skin disorders, including pityriasis folliculorum, folliculitis, rosacea, blepharitis, seborrheic dermatitis, and perioral dermatosis. Paeonol, with prolonged history of use in modern China, exhibits several beneficial effects such as anti-insect, anti-inflammatory, neuroprotective, antitumor, and anti-cardiovascular diseases.
Purpose
To evaluate the in vitro effectiveness of paeonol, the main component isolated from the root bark of Paeonia suffruticosa, against human Demodex.
Methods
A paeonol solution was prepared using paraffin oil, and human Demodex mites were obtained using the cellophane tape method. The drug solution was directly used to contact and kill both Demodex species in vitro.
Results
The experiment showed that 40 mg/mL was the minimum effective concentration of paeonol for killing the two mite species; paeonol exhibited more remarkable killing effect on D. brevis than on D. folliculorum.
Conclusions
This result suggests that paeonol has good in vitro acaricidal activity against human Demodex mite. Moreover, it is more effective against D. brevis than against D. folliculorum.
Prevalence and associated risk factors of prostate cancer among a large Chinese population
Authors: Chen Jun , He Liang , Ni Yixiu , Yu Feijun , Zhang Aokang , Wang Xing , Yan Junfeng 
Published: March 2024

Abstract

Prostate cancer (PCa) is one of the most important health problems among elderly in China, with the increasingly serious problem of aging. A cross-sectional survey was conducted in eastern China from 2022 to 2023. Recruitment included a total of 70342 participants aged 60 or older. Socio demographic profiles, examinations were administrated. Age-standardised prevalence rates were calculated by a direct method with a standard population. T test and chi-square test were used to compare the statistical differences. Multivariate regression models were used to identify the risk factors of PCa. Overall, the crude and age-adjusted PCa prevalence is 0.93% and 0.91%, respectively in eastern China. When we adjusted all the co-variables, it showed that elderly, smoking, having a higher BMI, smoking were associated with PCa. Faced with the epidemiological transition, we need innovative strategies to control and prevent PCa. Conducting early screening among male population in community health service centers in eastern of China, especially among population with high associated risk factors, such as elderly, overweight or obese and so on, and conducting community-based intervention programs might be helpful to address this serious problem.
Can be possible to predict motor and technical performance using multidimensional approach among adolescent male basketball players?
Authors: Mikołajec Kazimierz , Arede Jorge , Gryko Karol 
Published: March 2024

Abstract

The aims of the present study were (i) identify and compare the basic anthropometric characteristics, motor performance, game-related skills and functional capacity of under-15 (U-15) and under-16 (U-16) male basketball players, (ii) examine the influence of maturity offset on all performance parameters, (iii) predict test outcome in linear speed, agility, jumping, aerobic endurance and sport-specific skills. Two hundred thirty-four athletes volunteered participated in the study and completed a test battery including morphological variables (body height, body mass, standing reach), fitness (agility, jumping performance, aerobic endurance), technical skills (jump shot, free throws, dribbling) and functional movement screen. The U-16 group outperformed U-15 in terms of body height and mass, but also SVJmax and VJmax. Moreover, when data was adjusted considering chronological age, the group with more advanced maturity offset within U-15 group had achieved greater values in body mass, body height, arm reach and VJmax variable. Data analysis adjusted for age, body height and body mass with the reference to years after PHV showed that PHV2 group among U-15 basketball players had better jumping performance, and 20 m linear speed compared to PHV1 group. Maturity status was a relevant predictor of 20 m linear speed and SVJ tests. Other results indicate that chronical age (20 m speed, VO2max), body height (5, 10, 20 m speed, agility), body mass (agility), functional abilities (20 m speed, SVJ, VJ, VO2max) were significant predictors of physical performance. On the other hand, only body height (free throws) and FMS (jump shot, dribbling) manifested an impact on the level of sport-specific skills. The obtained results indicate that FMS test applied to young basketball players is not only right tool for injury prediction but also its outcome can influence technical performance. Therefore, we can conclude that composite score of < 14 out of 20 may result in low level of sport-specific capacity. It is significant evidence of practical application of FMS tests.
WITHDRAWN: The Tectonic Inversion Prediction in Fold-and-thrust Belts by Using Numerical Modeling
Authors: Zaidi Syeda Fakiha Ali , Ahsan Naveed 
Published: March 2024

Abstract
This research investigates to tectonic inversion in rifted continental margins, specifically focusing on the interaction between foreland sediment deposits and fold-and-thrust belts during orogeny. Using numerical modeling with ANSYS software and a Maxwell-type viscoelastic rheology, the study explores positive inversion in petroleum basins, revealing insights into the evolution of inverted basins. The research emphasizes the role of pre-existing extensional fault systems in controlling thrust faults, delving into the reactivation of faults and uplift of the hanging wall during tectonic compression. The study highlights diverse structural patterns associated with tectonic inversion in sub-thrust regions of fold-and-thrust belts, including anticlines, back-thrusts, fault propagation folds, pop-ups, and an inversion-related fracture pattern. Results demonstrate the critical influence of rheological properties in fault reactivation and deformational styles during tectonic inversion. Comparisons with natural case studies, like the Helvetic nappes in Switzerland, validate the predictive capability of numerical models for tectonic inversion structures in different geological settings, including the Kohat-Potwar Fold and Thrust Belt in Pakistan. The importance of understanding the interplay between geological structures and rheological properties for accurately predicting the evolution of inverted basins. The deeply study of tectonic inversion are extending to optimizing exploration efforts and interpreting structural complexities in petroleum exploration, providing valuable insights for reservoir prediction and fold-and-thrust belt structural evolution.
Diagnostic Efficacy of [ 99m Tc]Tc-PSMA SPECT/CT for Prostate Cancer: a Meta-Analysis
Authors: Wang Qi , Ketteler Svea , Bagheri Shamim , Ebrahimifard Ali , Luster Markus , Librizzi Damiano , Yousefi Behrooz Hooshyar 
Published: March 2024

Abstract

Background
Prompt and accurate diagnosis of prostate cancer (PCa) is of paramount importance for effective treatment planning. While Gallium-68 labeled prostate-specific membrane antigen (PSMA) positron emission tomography (PET)/computed tomography (CT) has proven efficacy in detecting PCa, limited availability poses challenges. As a potential alternative, [99mTc]Tc-PSMA single photon emission computed tomography (SPECT)/computed tomography (CT) holds promise. This systematic review and meta-analysis aimed to evaluate the diagnostic value of [99mTc]Tc-PSMA SPECT/CT for prostate cancer.
Methods
A comprehensive search of PubMed, Cochrane, EMBASE, and Web of Science databases was conducted until November 2023. Sensitivity and specificity data were extracted to assess the diagnostic accuracy of [99mTc]Tc-PSMA SPECT/CT, while the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool was used to evaluate study quality. Statistical analyses were performed using STATA 18, with MetaDisc 1.4 employed to detect threshold effects. Diagnostic accuracy indicators, including sensitivity, specificity, diagnostic odds ratio (DOR), negative likelihood ratio (LR-), and positive likelihood ratio (LR+), were pooled. The area under the curve (AUC) of the combined model was calculated using summary receiver-operating characteristic (SROC) curves.
Results
Six studies meeting the inclusion criteria were identified from an initial pool of 313 articles, with no publication bias observed. The pooled sensitivity, specificity, and AUC of [99mTc]Tc-PSMA SPECT/CT were found to be 0.90 (95% CI, 0.84–0.94), 0.83 (95% CI, 0.57–0.95), and 0.93 (95% CI, 0.91–0.95), respectively. Additionally, the comprehensive diagnostic odds ratio, diagnostic score, positive likelihood ratio, and negative likelihood ratio were calculated as 42.42 (95% CI, 11.21-160.58), 3.75 (95% CI, 2.42–5.08), 5.27 (95% CI, 1.80-15.41), and 0.12 (95% CI, 0.08–0.20), respectively.
Conclusions
In conclusion, our findings demonstrate that [99mTc]Tc-PSMA SPECT/CT exhibits favorable diagnostic performance for prostate cancer and can provide valuable supplementary information, particularly in regions and settings where [68Ga]Ga-PSMA PET/CT availability is limited, such as remote areas. These results highlight the potential of [99mTc]Tc-PSMA SPECT/CT as a valuable tool in the diagnosis and management of prostate cancer, warranting further investigation and validation in larger patient cohorts.
Effects of Non-Albuminuric Type 2 Diabetes Mellitus on Renal Outcomes: A Nationwide Study
Authors: Choi Dughyun , Park Samel , Kim Dong-Sub , Lee Dong-Jin , Cho Nam-Jun , Cho Yoon Young , Gil Hyo-Wook , Lee Eun Young 
Published: March 2024

Abstract

The specific impact of type 2 diabetes mellitus (T2D) on renal outcomes, independent of albuminuria, is not fully understood. This study aimed to investigate the renal outcomes in non-albuminuric T2D subjects compared to non-albuminuric non-T2D subjects. Data from the National Health Insurance Service-National Sample Cohort of Korea from 2009 to 2015 were analyzed. The non-albuminuric T2D group was compared with the non-albuminuric non-T2D group. The primary outcome was a composite renal outcome consisting of the initiation of renal replacement therapy and a decline of ≥ 40% in eGFR from baseline. Of 587,339 subjects, 77,267 were analyzed: 11,957 in the T2D group and 65,310 in the non-T2D group. The T2D group demonstrated a greater annual decline in eGFR of -0.113 ml/min/1.73 m2 (95% CI: -0.222 – -0.003, p = 0.044). The adjusted hazard ratio for the primary outcome was 1.57 (95% CI: 1.28¬–1.92, p < 0.001). The steeper decline in eGFR was particularly evident in patients with glomerular hyperfiltration (eGFR ≥ 120 ml/min/1.73 m2) and a duration of T2D exceeding 6 years. Non-albuminuric T2D was associated with a greater annual decline in renal function and had a 1.5-fold increased risk of adverse renal outcomes compared to non-albuminuric non-T2D.
Modulating proliferative and osteogenic potentials of mesenchymal stem cells via a novel formulation of MTA as a root perforation repair material
Authors: Elgendi Ahmed , Hassanien Ehab , Nagy Mohamed , Fathy Iman , Abu-Seida Ashraf , Hassanein Elhassan 
Published: March 2024

Abstract

Root perforations may cause periodontitis, resulting in alveolar bone loss. This study compared the zirconia containing MTA Matreva® (MAT group) to traditional MTA Angelus® (ANG group) as root perforation repair materials regarding the cytotoxicity and the osteogenic potential. Bone marrow mesenchymal stem cells (BM-MSCs) were isolated from two albino rats and cultured. Cytotoxicity of the tested materials was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay at three-time intervals; 3, 7 and 14 days. The tested materials were used to direct the osteogenic differentiation of BM-MSCs into osteoblasts. Evaluation of osteogenic effect was performed through assessments of calcium deposition by Alizarin red staining (ARS), expression of receptor activator of nuclear factor-kappa B (NF-κB) ligand (RANKL) protein in BM-MSCs seeded on the tested materials for 14 days and osteogenic potential gene markers expression in BM-MSCs after culture on the tested materials for 14 days. The assessed genes included; tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), and interleukin 1-β (IL-1β). All data were statistically analyzed. There were no significant differences between ANG and MAT groups in terms of cytotoxicity and osteogenic potential (P>0.05). The highest level of cell proliferation occurred in MAT groups, followed by ANG group, compared to negative controls (NC). The MAT group showed the highest levels of Average % calcific area fraction (90%) followed by that of ANG group (68%) as compared to NC (48%). The BM-MSCs in the MAT group exhibited significantly highest level of RANKL protein expression, followed by that of ANG group. The ANG group revealed significantly greater TNF-α and IL-1β levels than those in NC. The MAT group had significantly greater value of TGF-β than that of NC. Zirconia containing MTA (MTA Matreva) has comparable cytotoxicity and osteogenic potential to traditional MTA (MTA Angelus), which is regarded a dependable material for root perforation healing.
FGF21 inhibited NETs formation induced by myocardial I/R injury via AMPK
Authors: Gu Lingyun , Jia Chengao , Sheng Zuozhen , Jiang Wenlong , Xu Zhuowen , Li Weizhang , Zhang Hua 
Published: March 2024

Abstract

Background Previous investigations have established the anti-inflammatory properties of fibroblast growth factor 21 (FGF21). However, the specific mechanism through which FGF21 mitigates myocardial ischemia/reperfusion (I/R) injury by inhibiting neutrophil extracellular traps(NETs) formation remains unclear.
Methods A mice model of myocardial I/R injury was induced, and myocardial tissue was stained with immunofluorescence to assess the formation of NETs. Serum NETs levels were quantified using a PicoGreen kit. In addition, the expression levels of AMP-activated protein kinase (AMPK) and FGF21 were evaluated by Wes fully automated protein blotting quantitative analysis system. Moreover, an hypoxia/reoxygenation (H/R) model was established using AMPK inhibitor and agonist pretreated H9c2 cells to further explore the relationship between FGF21 and AMPK.
Results Compared with the control group, serum NETs levels were significantly higher in I/R mice, and a large number of NETs were formed in myocardial tissues (97.63±11.45 vs 69.65±3.33, P<0.05). However, NETs levels were reversed in FGF21 pretreated mice (P<0.05). Further studies showed that FGF21 enhanced AMPK expression, which was significantly increased after inhibition of AMPK and decreased after promotion of AMPK (P<0.05).
Conclusions FGF21 may exert cardioprotective effects by inhibiting I/R injury-induced NETs formation via AMPK.
Post-endoscopy esophageal squamous cell carcinoma with invasion of the muscularis mucosa or deeper detected in surveillance endoscopy after esophageal endoscopic resection
Authors: Kitagawa Daiki , Ishihara Ryu , Yoshii Shunsuke , Asada Yuya , Ueda Tomoya , Kizawa Atsuko , Ninomiya Takehiro , Okubo Yuki , Kawakami Yushi , Tani Yasuhiro , Kato Minoru , Shichijo Satoki , Kanesaka Takashi , Yamamoto Sachiko , Takeuchi Yoji , Higashino Koji , Uedo Noriya , Michida Tomoki , Fujiwara Yasuhiro 
Published: March 2024

Abstract

Background:Surveillance endoscopy is recommended after endoscopic resection of esophageal squamous cell carcinomas (ESCCs). However, surveillance endoscopy sometimes detects advanced metachronous ESCCs with invasion of the muscularis mucosa (MM) or deeper. We aimed to clarify the clinicopathological features of these advanced metachronous ESCCs.
Methods: This single-center retrospective study identified metachronous ESCCs detected during surveillance endoscopy. ESCCs that invaded the MM or deeper and were detected within 24 months after the previous endoscopy were defined as post-endoscopy esophageal advanced lesions (PEEALs), while the first ESCC detected in the patient was defined as the primary lesion. Study 1 compared the clinicopathological characteristics of PEEALs versus non-advanced lesions. Study 2 compared the endoscopic features of pT1a-MM PEEALs versus primary lesions.
Results: A total of 307 metachronous ESCCs were analyzed in Study 1. Of these, 20 were PEEALs and 287 were non-advanced lesions (pT1a-EP/LPM). The median intervals from the previous endoscopy for PEEALs and non-advanced lesions were 6.1 months and 6.7 months, respectively (P = 0.283). The morphological feature of marginal elevation was seen in 60% of PEEALs. In Study 2, 15 pT1a-MM PEEALs were compared with 149 pT1a-MM primary lesions. Compared with primary lesions, pT1a-MM PEEALs were smaller (median 10 mm vs. 30 mm, P <0.001) and had a higher prevalence of marginal elevation morphology (53.3% vs. 10.1%, P<0.001).
Conclusions: The specific features of PEEALs were a small diameter and marginal elevation. Surveillance endoscopy with careful observation for these lesions is recommended after endoscopic resection of ESCCs.
Trial registration: The present study is a non-intervention trial.
Combined Empowerment, Continuous Appraisal, Psychological and Career Counseling Improve Medical Cadets’ Satisfaction and May Offset Burnout During Internship Year. An 18-Months Successful Experience in a Tertiary Medical Center
Authors: Shoham Reut , Robinson Hanni , Yehiel Noy , Robinzon Vered , Frenkel Yael Nir , Segal Gad 
Published: March 2024

Abstract

Background.
Following graduation from school of medicine, Israeli medical cadets complete a 12-months training period in hospitals. This year, their jump-start of career, has profound influence on their future contribution to our national workforce. The crisis in the healthcare system is already notable in this group of physicians, with burnout, sense of personal and professional uncertainty and disorientation resulting from the nature of their changing environment.
Methods.
In the largest Israeli tertiary hospital, we established an education authority, overseeing cadets as trainees rather workers. We offer them constructed, personalized psychological and counselling services with continuous process of appraisal. During an 18-month period we followed their monthly feedback relating to their satisfaction, learning experience and level of socialization with their peers and superiors. The study was designed as comparative research assessing cadets’ satisfaction measurements before, and after intervention as a surrogate marker for their burnout.
Results.
Comparison of measured parameters amongst our study population (before and after intervention) showed statistically significant improvement, with interns stationed in the surgical departments (n = 86) showing the highest degree of improved satisfaction: the extent of acquiring new knowledge and competencies [Likert questionnaire results (1 to 5 LQR) went from 2.2 ± 1.0 to 3.3 ± 1.42 ; p < 0.005]; experience from the absorption process into the department [LQR from 2.6 ± 1.2 to 3.5 ± 1.56 ; p < 0.05], degree of motivation to recommend peers to apply for residency [LQR from 2.3 ± 1.0 to 3.1 ± 1.6 ; p = 0.05] and the experience of being accepted to the department by the staff nurses [LQR from 2.7 ± 1.3 to 3.5 ± 1.1 ; p < 0.05].
Conclusions.
A combined, ongoing process of appraisal, empowerment, psychological and career counseling seems promising in the relenting effort to improve cadets’ satisfaction and hopefully withhold the burnout process of young physicians.
MULTIMORBIDITY, MORBIDITIES, AND LONG COVID – findings of the Sulcovid longitudinal study
Authors: Ventura Lucas Souza , Vieira Yohana Pereira , Rocha Juliana Quadros Santos , Fehlberg Lorrany da Silva Nunes , de Souza Cristiane , Duro Suele Manjourany Silva , Saes Mirelle de Oliveira 
Published: March 2024

Abstract

Background
The aim of this study was to evaluate the association between long COVID, morbidities, and multimorbidity in adults and older adults six to nine months after infection with the SARS-CoV-2 virus in Southern Brazil.
Methods
Baseline data were obtained from the longitudinal study, Sulcovid, conducted on individuals diagnosed with COVID-19 through RT-PCR testing from December 2020 to March 2021, who were symptomatic and living in a city in southern Brazil. Long COVID was assessed based on the affirmative response to at least one of the 18 symptoms investigated and categorized as musculoskeletal, neurological, respiratory, sensory, or digestive. Morbidities were assessed based on the presence of at least one of nine self-reported diseases. Data were analyzed using the Stata 15.0 statistical package. Crude and adjusted analyses were performed using Poisson regression to assess the relationships between morbidity, multimorbidity, and long COVID.
Results
In total, 2,919 people were interviewed. The most prevalent morbidities were anxiety (26.3%), hypertension (25.3%), and depression (19.4%). In addition, 17.8% reported two previous morbidities and 22.6% had three or more comorbidities. Individuals with depression (PR = 1.17 95% CI 1.05–1.30), anxiety (PR = 1.33 95% CI 1.21–1.47), two or more morbidities (PR = 1.22 95% CI 1.07–1.39), and three or more morbidities (PR = 1.40; 95% CI 1.24–1.57) were more likely to have long COVID. A linear trend was observed, where individuals with two and three or more morbidities were 1.22 (95% CI 1.07–1.39) and 1.40 (95% CI 1.24–1.57) times more likely to develop long COVID than those with no or one morbidity.
Conclusions
The findings of this study reinforce that individuals with morbidities and multimorbidities prior to infection had greater vulnerability to long COVID.