bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purinergic system molecules: expression and regulation of enzymes, transporters, and adenosine triphosphate receptors and the role of adenosine triphosphate at the maternal–conceptus interface in pigs
Authors: Cheon Yugyeong , Yoo Inkyu , Lee Soohyung , Choi Eunhyeok , Kim Seonghyun , Ka Hakhyun 
Published: February 2025

Abstract
The purinergic system is composed of purine nucleotides, enzymes, transporters, and receptors and is involved in a variety of physiological processes in the body, including development, metabolism, immunity, tumorigenesis, and reproduction. The importance of the purinergic system for embryo implantation in the endometrium and in pathophysiological conditions has been shown in some species. However, the expression, regulation, and function of purinergic system molecules at the maternal–conceptus interface in pigs is not fully understood. Therefore, we determined the expression of purinergic system molecules in the endometrium during the estrous cycle and pregnancy and in the conceptus and chorioallantoic tissues during pregnancy in pigs. The expression of many purinergic system molecules in the endometrium changed dynamically during pregnancy, with the expression of several components in the endometrium greater on day 15 of pregnancy than day 15 of the estrous cycle, and it was regulated by conceptus-derived interleukin-1beta and interferon-gamma. Purinergic system molecules were also expressed in conceptuses during early pregnancy and in chorioallantoic tissues during mid- to term pregnancy. Furthermore, adenosine triphosphate, a major purinergic signaling molecule, increased migration of endometrial epithelial and conceptus trophectoderm cells, the expression of prostaglandin synthetic enzymes, and the secretion of prostaglandin F2alpha in endometrial epithelial cells in vitro. These data suggest that the purinergic system molecules expressed in the endometrium, conceptus, and chorioallantoic tissues might play an important role in the establishment and maintenance of pregnancy by regulating various cellular functions at the maternal–conceptus interface in pigs.
Clinical Characteristics and Outcomes of Small Bowel Neoplasms in Crohn’s Disease: A Case-Control Study
Authors: Urquhart Siri A , Smyrk Thomas C , Harmsen William S , Loftus Edward V , Kisiel John B , Coelho-Prabhu Nayantara 
Published: February 2025

Abstract

Background
Patients with Crohn’s disease (CD) who have ileal or any small bowel (SB) involvement are at increased risk of developing SB cancer. Due to the rarity of this complication of CD, we aimed to describe the clinical features, presentation, and of small bowel neoplasms (SBN) in patients with CD.

Methods
A case-control study was performed to include patients ≥18 years old with a diagnosis of CD with or without SBN at a single large referral center from January 1992 to May 2023. Patients were identified using bioinformatics and natural language processing tools, as well as anatomic pathology records. Two age- and sex-matched controls were identified for each case.

Results
In total, 54 patients with CD and SBN and 108 patients with CD without SBN were identified. Of the cases, most had ileal CD (55.6%) with stricturing (59.3%) phenotype. Median duration of CD prior to SBN diagnosis was 19.5 years. Nonpenetrating/nonstricturing behavior (odds ratio [OR], 9.23; 95% CI, 2.91-29.32; P = .0008) was significantly associated with an increased odds of SBN. History of tobacco use (OR, 0.27; 95% CI, 0.13-0.60; P = .0011) and IBD-associated colonic neoplasia (OR, 0.18; 95%, CI 0.4-0.85; P = .0303) were protective in development of SBN.

Conclusions
Nonpenetrating/nonstricturing CD appeared to raise SBN risk. History of tobacco use and colonic IBD-associated neoplasia are associated with reduced risk of SBN. Further studies with large sample sizes are needed to determine true incidence and risk factors associated with SBN in CD and assess potentially protective effects of early surgery.
Multimodal lifestyle intervention improves fatigue in quiescent Inflammatory Bowel Disease: a controlled study
Authors: Loveikyte Roberta , Koppelman Lola J M , Blijleven Mirjam J H , Wilmsen Nathalie , Rodríguez – Girondo Mar D M , Bloem Sjaak , Voorneveld Philip W , van der Meulen – de Jong Andrea E , van der Marel Sander , Maljaars P W Jeroen 
Published: February 2025

Abstract

Background
Lifestyle factors are significant contributors to fatigue, affecting ~45% of patients with Inflammatory Bowel Disease (IBD). Hence, we evaluated the effect of a multimodal lifestyle intervention on fatigue in patients with IBD.

Methods
Patients with quiescent IBD were enrolled in this multicenter, non-randomized, controlled interventional study. The intervention group followed a 12-month lifestyle program, which included digital group meetings with a nutritionist and a lifestyle coach focusing on nutrition, exercise, sleep, and relaxation. The program also encouraged patients to exercise more self-control over personal health. The control group received standard clinical care. Clinical data and patient-reported outcomes were collected. Fatigue was measured with the Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-F); any increase in FACIT-F was considered a positive response to the intervention. Inverse probability treatment weighting was used to correct confounding by indication.

Results
Thirty-six patients in the intervention group and 32 in the control group were compared. More patients in the intervention group (82.1%) than in the control group (54.2%) experienced improvement in fatigue, P = 0.029, Standardized Mean Difference (SMD) -0.624. Over 70% of patients in the intervention group achieved a clinically relevant improvement in fatigue. Compared to the control group, quality of life improved in the intervention group. Acceptance of the health status was a significant factor for fatigue improvement (β 7.899, SE 1.913, P &lt; 0.001).

Conclusions
Multimodal lifestyle intervention improves fatigue in patients with IBD. Acceptance appears essential for fatigue improvement; instruments evaluating acceptance could help to personalize treatment and maximize its effectiveness.
Impact of loading, heart rate and short episodes of ischaemia on myocardial stiffness assessed using shear wave elastography in an open-chest animal model
Authors: Saloux Eric , Simard Christophe , Ruello Pauline , Lemaitre Adrien , Hodzic Amir , Lebrun Alexandre , Dupont Pierre-Antoine , Tribouilloy Christophe , Eltchaninoff Hélène , Le Garec Morgane , Fraschini Christophe , Saplacan Vladimir , Manrique Alain 
Published: February 2025

Abstract

Aims
Shear Wave Elastography (SWE) is a new promising ultrasound modality that enables non-invasive measurement of the dynamic myocardial stiffness. The impact of varying physiological conditions on SWE measurement of left ventricular (LV) myocardial stiffness remains poorly investigated.

Methods and results
Nineteen sheep were evaluated during open-chest surgery. Epicardial multiframe SWE acquisitions were performed in short axis view simultaneously with hemodynamic acquisitions during inferior vena cava occlusion, aortic clamping, atrial pacing and ischaemia-reperfusion. The cyclic variation in the median value of LV myocardial stiffness ranged from 1.1 m/s in diastole (Cmin) to 2.4 m/s in systole (Cmax). At steady state, intra-animal reproducibility was good for Cmin (intraclass correlation coefficient ICC= 0.77 [0.54, 0.90], p &lt; 0.001) and Cmax (ICC= 0.92 [0.84, 0.96], p &lt; 0.001). Cmin was independent of loading conditions, heart rate and short 15-minute episodes of ischaemia and reperfusion. Cmax was independent of loading conditions and moderate increase in heart rate but decreased significantly during ischaemia and reperfusion. Compared to baseline, percentage changes in Cmax was correlated to percentage changes in dP/dtmax (R=0.47, p = 0.001) and in LV systolic pressure (R=0.35, p=0.013) and SW (R=0.31, p=0.026).

Conclusion
In this study, LV diastolic myocardial stiffness Cmin assessed using SWE demonstrated the characteristics of a potentially useful clinical marker of LV diastolic function linked to the intrinsic elastic properties of the myocardium, whereas Cmax was an indicator of LV contractility.
Obtaining complete and canonical ammonia-oxidizing bacteria through specific labelling and cell sorting
Authors: Blom Pieter , Huizing Pascal C , de Monlevad João P R C , van Kessel Maartje A H J , Lücker Sebastian 
Published: February 2025

Abstract
Mitigation of the negative environmental consequences of excess anthropogenic nitrogen input requires a thorough understanding of the processes driving the biogeochemical nitrogen cycle. Nitrification is one of the key nitrogen-cycling processes and is performed by ammonia-oxidizing bacteria and archaea, nitrite-oxidizing bacteria, and complete nitrifiers. However, the fastidious growth of nitrifiers largely hampered their isolation using classical cultivation techniques, as most nitrifiers do not grow on solid media. Here, we present a workflow for the targeted enrichment and isolation of complete and canonical ammonia-oxidizing bacteria by combining function-specific in vivo fluorescent labelling with cell sorting. Optimized floc disruption and labelling techniques enlarged the fraction of planktonic cells and the fluorescent signal intensity, respectively, while maintaining cell viability. Sorted fractions were incubated in ammonium-containing mineral media and were screened for nitrite and nitrate production. Nitrifying cultures were upscaled and characterized with 16S rRNA and amoA gene-targeted PCRs and fluorescence in situ hybridization. Overall, we obtained one axenic and one enriched Nitrosomonas, and seven comammox Nitrospira enrichment cultures from five bioreactors, a recirculating aquaculture system biofilter, and agricultural soil. In conclusion, the presented workflow enables the fast and targeted retrieval of ammonia oxidizers from complex samples, allowing for in-depth physiological characterization.
Artificial neural network technique for simulation of improved thermal energy of Reiner–Philippoff nanofluid over a stretching curved surface
Authors: Aldandani Mohammed , Jan Ahmed , Alsaeed Salman S , Farooq Umer 
Published: February 2025

Abstract
In mechanical engineering and industrial applications, single-walled carbon and multi-walled nanotubes are becoming more popular because of their direct impact on improving the thermal conductivity of base fluids. Considering the fascinating characteristics of carbon nanotubes (CNTs), the objective of this study is to examine the influence of radiation on the movement of nanofluids suspended in CNTs across a stretched sheet affected by slip state, while a magnetic field is present. Combining neural networks with the backpropagation technique based on the Levenberg–Marquardt scheme is a new computer model that this study proposes. Applying it to the flow of non-Newtonian Riner–Philippoff boundary layers via a curved tensile plate allows one to derive non-linear systems from the governing equations and solve them. Furthermore, the partial differential equations for the magnetohydrodynamics boundary layer flow across a curved stretched sheet are transformed into non-similar dimensionless partial differential equations, which are then handled as ordinary differential equations using the local non-similarity method and solved using the bvp4c MATLAB tools. By adjusting the following embedding settings, a variety of scenarios are used to generate an input data set for the presented Levenberg Marquardt scheme- Back-Propagation Neural Network (LMS-BPNN) model: Bingham, Riener–Philippoff fluid, and magnetic parameter. To compare the produced scenarios' outcomes with the reference results, we assess the LMS-BPNN model's training, testing, and validation. The efficacy and performance of the LMS-BPNN infrastructure model are assessed using a variety of metrics for the fluidic system convergence study, among which are regression graphs, an error histogram, and the mean square error (MSE). Using the built-in scenarios, we evaluate the training, testing, and validation of the LMS-BPNN model while assessing the findings to the standard outcomes. MSE, error histogram, and regression plots are used to evaluate the LMS-BPNN infrastructure model for the fluidic system convergence study.
Combined internal fixation and autologous chondrocyte implantation for tibial plateau fracture concomitant with cartilage injury: a case report
Authors: Sogi Yasuhito , Ohnuma Masahiro , Kunii Tomonori , Takahashi Atsushi , Aizawa Toshimi 
Published: February 2025

Abstract
This is the first report of tibia plateau fracture (TPF) concomitant with a large cartilage injury, which was treated with consecutive procedures comprising internal fixation and autologous chondrocyte implantation (ACI). A 49-year-old male patient was diagnosed with a medial tibial plateau fracture concomitant with a large medial femoral condyle cartilage injury. The patient underwent internal fixation and ACI at a 4-week interval. One year after the surgery, he could resume daily activities and sports. Magnetic resonance imaging and arthroscopic findings showed that the injured cartilage was covered by hyaline cartilage-like tissue. TPF concomitant with large cartilage injury can be treated with consecutive internal fixation and ACI procedures, shortening the overall treatment period and favorable outcomes.
Social Media Engagement and Quit Intentions among Youth with Exclusive E-cigarette Use: The Moderating Role of Social Norms
Authors: Jebai Rime , Hong Traci , Ranker Lynsie R , Wu Jiaxi , Rohila Aarushi , Fetterman Jessica L , Cornacchione Ross Jennifer 
Published: February 2025

Abstract

Introduction
E-cigarette use is prevalent among US youth. Little is known about the association between engagement with e-cigarette-related social media posts, vaping norms, and intention to quit e-cigarettes among youth who exclusively use e-cigarettes.

Methods
A cross-sectional national online survey was conducted among a sample of youth aged 13-18 who reported exclusive e-cigarette use in the past 30 days. Logistic regression was used to estimate the association between engagement with e-cigarette-related posts on social media, vaping social norms, and intention to quit e-cigarettes. Two interaction terms were added between descriptive and injunctive norm variables and engagement with social media posts, respectively, to test the potential differential effect of social norms on intention to quit. Models were adjusted for demographic and e-cigarette use variables.

Results
The sample (N=143) was 62% females; 69% identified as White and 20% as Black. Engaging with e-cigarette-related posts on social media was associated with higher odds of an intention to quit e-cigarettes compared to those who never engaged with e-cigarette posts [adjusted aOR=2.70, 95%CI (1.13,6.42)]. Intention to quit e-cigarettes was lower among youth who believed the use of e-cigarettes was common (descriptive norms) [0.71, (0.57,0.88)], and who described the views of people important to them as positive (vs. negative; injunctive norms) [0.26, (0.07,0.98)]. Youth who engaged with posts and had positive views from people important to them on e-cigarettes (injunctive norms) were less likely to express an intent to quit vaping (aOR=0.10; p-value=0.0394).

Conclusions
Social media exposure and social norms may influence quit intentions among youth who exclusively use e-cigarettes. Targeted interventions on social media to address the potential of these factors in promoting cessation behaviors are needed.

IMPLICATIONS
Our study highlights the interplay between social media engagement, descriptive and injunctive social norms of vaping, and intention to quit e-cigarette use among youth who exclusively use e-cigarettes. These insights are crucial for designing effective, targeted social media-based cessation interventions, promoting quitting intentions, and addressing misperceptions about vaping, thereby supporting youth cessation behaviors
Intrinsic stiffness and Θ-solvent regime in intrinsically disordered proteins: Implications for liquid–liquid phase separation
Authors: Baidya Lipika , Kremer Kurt , Reddy Govardhan 
Published: February 2025

Abstract
Liquid–liquid phase separation (LLPS) exhibited by intrinsically disordered proteins (IDPs) depends on the solvation state around the Θ-regime, which separates good from poor solvent. Experimentally, the Θ-solvent regime of the finite length (N) IDPs, as probed by small angle X-ray scattering (SAXS) and single molecular fluorescence resonance energy transfer (smFRET), is in disagreement. Using computer simulations of a coarse-grained IDP model, we address the effect of chain length on the Θ-regime of IDPs with polar side chains (polyglutamine) and hydrophobic side chains (polyleucine) subject to varying concentrations of cosolvents [C], urea (denaturant) or trimethylamine N-oxide (protective osmolyte) in water. Due to their intrinsic stiffness, these IDPs are always expanded on short-length scales, independent of the solvent quality. As a result, for short IDP sequences (≈10 to 25 residues), their propensity to exhibit LLPS cannot be inferred from single-chain properties. Further, for finite-size IDPs, the cosolvent concentration to attain the Θ-regime ([CΘ]) extracted from the structure factor emulating SAXS and pair distances mimicking smFRET differs. They converge to the same cosolvent concentration only at large N, indicating that finite size corrections vary for different IDP properties. We show that the radius of gyration (Rg) of the IDPs in the Θ-solvent regime satisfies the scaling relation Rg2=Nf(cN), which can be exploited to accurately extract [CΘ] (c=([C]/[CΘ]−1)). We demonstrate the importance of finite size aspects originating from the chain stiffness and thermal blob size in analyzing IDP properties to identify the Θ-solvent regime.
A Case of Recurrent Eyelid Cutaneous Myxoma With Basaloid Proliferation in a Child
Authors: Stoieva Margaryta , O'Donnell Emily 
Published: February 2025

Abstract:
Cutaneous myxomas are extremely rare in the pediatric population. We describe a case of an eyelid cutaneous myxoma with multiple recurrences in an 8-year-old child. The lesion once previously diagnosed as eccrine hidrocystoma had been excised three times with subsequent recurrence. Frozen section diagnosis performed during the fourth excision showed striking basaloid features, prompting deferral of definitive diagnosis to permanent sections. Following routine processing, basaloid proliferations were again seen without significant atypia or mitotic activity in addition to background features of myxoma. CD10 immunostain highlighted both basaloid nests and surrounding stroma. Cutaneous myxoma was diagnosed. The diagnosis was corroborated by an external consultation. The patient underwent genetic testing for the Carney complex with negative results. This case report raises some practical points. First, due to their rarity in the pediatric population and potential to mimic other entities, cutaneous myxomas can be misdiagnosed as other benign neoplasms. Second, although induction of basaloid proliferation has been well-documented in myxoma, it can be easily misinterpreted as a malignant process, particularly in the setting of a frozen section diagnosis and lack of available clinical information. Third, even though solitary cutaneous myxomas are less likely to be associated with the Carney complex, a possibility of such an association should always be born in mind, as consequences of missing syndromic neoplasms, such as cardiac myxoma, can be lethal.
Efficacy of 3D-Printed Cranial Remolding Orthoses for Infants in Australia
Authors: Graham Tiffany , Wang Jijia 
Published: February 2025

ABSTRACT

Introduction
Cranial remolding orthoses (CROs) have been used to treat various types of infantile nonsynostotic cranial deformation worldwide, but little is known about the treatment outcomes with the newer 3D-printed orthoses.

Objectives
This study examines the efficacy of 3D-printed cranial orthoses in the Australian population by retrospectively examining treatment outcomes for infants with deformational head shapes and developing predictive arrays that may have clinical utility.

Study Design
This is a retrospective chart review of infants treated at a multisite clinic in Australia between 2019 and 2023.

Methods
Subjects were divided into groups according to their presenting severity and changes in cephalic measurements over the course of treatment. Results were stratified by initial head shape type and presented in terms of measurement change per millimeter of cranial circumferential growth. Predictive arrays were created using expected cranial circumferential growth for typically developing infants and mean treatment results based on initial head shape and infantile age.

Results
A total of 257 infants were fit with 3D-printed CROs (initial cranial vault asymmetry index range = 0.21 to 19.59 and initial cephalic ratio range = 73.2% to 107.86%). All infants saw improvement in their cranial shape. Predictive arrays were developed to assist with estimating clinical expectations of treatment results based on 4 months of cranial growth for typically developing infants.

Conclusions
Results suggest 3D-printed cranial orthoses are effective in reducing cranial deformation in the Australian infantile population.

Clinical Relevance
3D-printed CROs are relatively new and seem to be effective in reducing cranial deformation. The predictive arrays may be a useful quick reference tool for practitioners to determine if it is likely for an infant to achieve full cranial correction in 4 months based on the infant’s presenting age and severity.
Predicting disease-free survival following curative-intent resection of right-sided colon cancer using a pre- and post-operative monogram: a prospective observational cohort study
Authors: Lucocq James , Trinder Tom , Homyer Kate , Baig Hassan , Patil Pradeep , Muthukumarasamy Girivasan 
Published: February 2025

Introduction:
Disease prognostication can be achieved through the derivation of biologically and clinically integrated prediction models. The present study reports 1-, 3-, and 5-year disease-free survival (DFS) in patients undergoing right hemicolectomy for curative intent and both derives and validates a pre- and post-operative prediction tool for DFS for prognostication and risk stratification purposes.

Method:
Consecutive patients undergoing right-sided curative-intent resection for colorectal cancer (2010–2020) in a tertiary care unit were followed-up prospectively for recurrence and survival outcomes. Survival analyses were used to derive pre- and post-operative models predicting 1-, 3-, and 5-year DFS. Calibration was reported and internal validation was performed using bootstrapping.

Results:
A total of 822 patients underwent resection and 528 had ≥5-year follow-up. The 1-, 3-, and 5-year DFS rates were 85.6%, 72.5% and 57.6%, respectively. Variables associated with death/recurrence included: increasing age (HR > 1.95, P = 0.037), male gender (HR 1.62, P < 0.001), ASA ≥3 (HR 1.79, P < 0.001), low albumin (HR 1.54, P < 0.001), T4 stage (HR 2.35, P = 0.023), R1 status (HR 1.63, P = 0.024), ≥4 positive lymph nodes (HR > 1.74, P < 0.001) and Clavien-Dindo ≥3 (HR 2.83, P < 0.001). The pre- and post-operative models contained 9 and 13 demographic, clinical, biochemical, operative and pathological variables, respectively (C-index 0.75 and 0.78, respectively). Excluding demographic, clinical and operative variables significantly reduced the C-index of the pre- (0.62) and post-operative models (0.69).

Conclusion:
The presented prediction tools for DFS will help clinicians stratify risk, offer appropriate adjuvant treatment and predict long-term survival following curative-intent right-sided colon cancer resection.