bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of age and diet consistency on the expression of myosin heavy‐chain isoforms on jaw‐closing and jaw‐opening muscles in a rat model
Authors: Schaub Leandra , Lagou Aikaterini , Ait‐Lounis Aouatef , Kiliaridis Stavros , Antonarakis Gregory S. 
Published: March 2024

AbstractBackgroundSkeletal craniofacial morphology can be influenced by changes in masticatory muscle function, which may also change the functional profile of the muscles.ObjectivesTo investigate the effects of age and functional demands on the expression of Myosin Heavy‐Chain (MyHC) isoforms in representative jaw‐closing and jaw‐opening muscles, namely the masseter and digastric muscles respectively.MethodsEighty‐four male Wistar rats were divided into four age groups, namely an immature (n = 12; 4‐week‐old), early adult (n = 24; 16‐week‐old), adult (n = 24; 26‐week‐old) and mature adult (n = 24; 38‐week‐old) group. The three adult groups were divided into two subgroups each based on diet consistency; a control group fed a standard (hard) diet, and an experimental group fed a soft diet. Rats were sacrificed, and masseter and digastric muscles dissected. Real‐time quantitative polymerase chain reaction was used to compare the mRNA transcripts of the MyHC isoforms—Myh7 (MyHC‐I), Myh2 (MyHC‐IIa), Myh4 (MyHC‐IIb) and Myh1 (MyHC‐IIx)—of deep masseter and digastric muscles.ResultsIn the masseter muscle, hypofunction increases Myh1 (26, 38 weeks; p < .0001) but decreases Myh4 (26 weeks; p = .046) and Myh2 (26 weeks; p < .0001) expression in adult rats. In the digastric muscle, hypofunction increases Myh1 expression in the mature adult rats (38 weeks; p < .0001), while Myh2 expression decreases in adult rats (26 weeks; p = .021) as does Myh4 (26 weeks; p = .001). Myh7 expression is increased in the digastric muscle of mature adult rats subjected to hypofunction (38 weeks; p = <.0001), while it is very weakly expressed in the masseter.ConclusionIn jaw‐opening and jaw‐closing muscles, differences in myosin expression between hard‐ and soft‐diet‐fed rats become evident in adulthood, suggesting that long‐term alteration of jaw function is associated with changes in the expression of MyHC isoforms and potential fibre remodelling. This may give insight into the role of function on masticatory muscles and the resultant craniofacial morphology.
Pain and salivary biomarkers of stress in temperomandibular disorders were affected by maxillary splints
Authors: Klepzig K. , Wendt J. , Teusch L. , Rickert C. , Kordaß B. , Lotze M. 
Published: March 2024

AbstractBackgroundLongitudinal intervention studies on treatment options in temporomandibular dysfunction (TMD) including self reports and salivary biomarkers of stress are rare and the exact therapeutic function of occlusal splints widely unknown.MethodsWe examined the therapeutic effects of a Michigan splint with occlusal relevance in patients with TMD using a placebo‐controlled, delayed‐start design. Two intervention groups received a Michigan splint, while one of them had a placebo palatine splint for the first 3 weeks. We collected pain intensities (at rest and after five occlusal movements), salivary measures associated with stress (cortisol and alpha‐amylase) and self‐reported psychological distress (stress, anxiety, catastrophizing) at baseline and 3 and 7 weeks after onset of intervention.ResultsAt baseline, we observed increased pain intensity and psychological distress in TMD patients compared to 11 matched healthy controls. Baseline anxiety was linked to movement pain intensity through stress. Over therapy reductions in pain intensity and morning cortisol were more pronounced in those patients starting immediately with the Michigan splint, while psychological distress decreased similarly in both groups.ConclusionOur results suggest that perceived stress plays a role for the association between anxiety and TMD pain and underlines the need for an interdisciplinary perspective on the pathogenesis and therapy of TMD in a setting where psychotherapeutic knowledge is still scarce or rarely applied.
Exploring adolescent‐adult connections, coping, and safety among minoritized youth in neighborhoods impacted by community violence
Authors: Fenner Maxine , Wilson Tyia , Riley Alexander , Culyba Alison J. 
Published: March 2024

AbstractDyadic interviews were conducted with 32 youth ages 13–21 and their self‐identified key adult supports to illuminate how adult supports help protect youth in communities impacted by high levels of violence. Interview transcripts were analyzed using thematic content analysis. Youth described choosing social interactions carefully, avoiding high‐violence areas, and keeping busy with activities. Many youths discussed the necessity of minimizing contact with peers to avoid violence, resulting in isolation from friends and increased engagement with family at home. Adult supports reflected upon an intergenerational transfer of violence avoidance, safety planning, and coping strategies through sharing their own lived experiences. Dyads highlighted the need for intergenerational programming to address social isolation and build supportive social networks.
Plant parasitic nematodes on soybean (Glycine max (L) Merr.) in the Metekel zone (Ethiopia) and varietal resistance evaluation to Meloidogyne javanica
Authors: Adamu Frezer A. , Hundessa Wakuma B. , Getachew Sihen , Aseffa Abebe W. , Meressa Beira H. 
Published: March 2024

AbstractPlant parasitic nematodes cause severe damage to soybean crops, leading to substantial losses in yield and quality. However, more information is needed regarding the occurrence, distribution and damage status of soybean PPNs in Ethiopia. A total of 180 samples of rhizosphere and roots were collected randomly from three major soybean‐producing districts of Metekel Zone. Nematodes were extracted and identified at the genus level based on their morphological characteristics, except Meloidogyne javanica, the deadly species, which was identified using morphological and molecular techniques. Seven nematode genera were recovered: Meloidogyne, Scutellonema, Pratylenchus, Helicotylenchus, Rotylenchulus, Hemicycliophora and Criconema. The genera Meloidogyne, Scutellonema and Pratylenchus were dominant and had FO% of 98.3%, 97.5% and 81.8%, respectively. The host status of 10 soybean varieties to M. javanica infection was determined. Data on the shoot height, root length, fresh shoot and root weight, gall number and nematode density were estimated 3 months after inoculation. The results revealed that Jalele variety suppresses M. javanica multiplication while the other nine supported it. Comparatively, Pawe‐3, Awassa‐95 and Koreme varieties supported the highest nematode multiplication 24.4, 16.2 and 15.3 reproductive factors, respectively. Nova experienced the highest shoot and root relative biomass losses (84%) and roots (63%). In contrast, Jalele did not experience any loss in all parameters. However, Jalele variety resisted M. javanica, making it a promising approach towards soybean breeding programs for nematode management.
Synthesis: International perspectives on healthcare for people with intellectual and developmental disabilities
Authors: Breuer Marian E. J. , Pelle Tim , Leusink Geraline L. , Linehan Christine , Naaldenberg Jenneken 
Published: March 2024

AbstractBackgroundThe organization of healthcare for people with intellectual and developmental disabilities (IDD) varies across countries. Each country has developed unique practices embedded in their historical and organizational context. Understanding and sharing these practices across borders facilitates mutual understanding about healthcare needs of people with IDD and facilitates the adoption of effective strategies in other countries.AimTo provide a synthesis across the country‐specific papers in the JPPID special edition and thereby identify underlying trends, challenges, and best practices in healthcare for people with IDD.MethodsThe papers in this special edition, which describe the organization of healthcare for people with IDD in 13 countries, were qualitatively analyzed using thematic content analysis, focusing on general characteristics, history, and context, organization of healthcare for people with IDD, challenges, and best practices.ResultsEach paper described a specific national history of evolution of healthcare for people with IDD, but our analysis showed that countries face similar challenges in healthcare for people with IDD. These challenges cover (1) access to healthcare, (2) quality of healthcare, (3) implementation, and (4) visibility of people with IDD. Consequently, people with IDD continue to face significant health disparities. Several best practices have been developed, ranging from making mainstream healthcare more accessible and suitable to providing specialized services, and advocating and raising awareness.ConclusionsThis synthesis is the first paper to include perspectives on healthcare for people with IDD across 13 countries. We identified that, despite differences in context, countries face similar challenges in improving healthcare for people with IDD. International collaboration and networking can provide essential tools in reducing health disparities that people with IDD face, starting with the challenges identified in this synthesis. This will require effort to especially include low‐ and middle‐income countries.
Multi‐omics provides insights into genome evolution and betacyanin biosynthesis in the halophyte Suaeda salsa
Authors: Wang Xin , Xia Jiang‐Bao , Bai Jun‐Hong , Yin Shuo , Wang Wei , Wang Da‐Wei , Yi Xin‐Xin , Dai Sheng‐Hong 
Published: March 2024

AbstractAs an important halophyte in the Yellow River Delta, the Amaranthaceae C3 Suaeda salsa (L.) Pall. has attracted much attention for the “red carpet” landscape, and could be simply divided into red and green phenotypes according to the betacyanin content in the fleshy leaves. However, S. salsa has not been sequenced yet, which limited people's understanding of this species at the molecular level. We constructed a high‐quality chromosome‐scale reference genome by combining high‐throughput sequencing, PacBio single molecule real‐time sequencing, and Hi‐C sequencing techniques with a genome size of 445.10 Mb and contigs N50 of 2.94 Mb. Through the annotation of the S. salsa genome, 298.76 Mb of the repetitive sequences and 23 965 protein‐coding genes were identified, of which the proportion of long terminal repeats type in the repetitive sequences was the most abundant, about 50.74% of the S. salsa genome. Comparative genomics indicated that S. salsa underwent a whole‐genome duplication event about 146.15 million years ago (Ma), and the estimated divergence time between S. salsa and Suaeda aralocaspica was about 16.9 Ma. A total of four betacyanins including betanidin, celosianin II, amaranthin and 6′‐O‐malonyl‐celosianin II were identified and purified in both phenotypes, while two significantly up‐regulated betacyanins (celosianin II and amaranthin) may be the main reason for the red color in red phenotype. In addition, we also performed transcriptomics and metabolomics in both phenotypes to explore the molecular mechanisms of pigment synthesis, and a series of structural genes and transcription factors concerning with betacyanin production were selected in S. salsa.
Qineryangia, a new genus from the Hengduan Mountains and new insights into the phylogeny of the subtribe Crepidinae (Cichorieae, Asteraceae)
Authors: Xu Lian‐Sheng , Song Zhu‐Qiu , Liao Shu‐Yuan , Chen You‐Sheng 
Published: March 2024

AbstractThe Crepidinae are the largest subtribe of the Cichorieae (Asteraceae). Debate remains over the circumscription and phylogeny of this subtribe, mainly due to its complex morphology and the poor phylogenetic signal provided by traditional Sanger sequencing markers. In this study, a well‐resolved phylogeny of the subtribe Crepidinae, consisting of seven highly supported clades, was obtained for the first time using nuclear data with a phylogenomics approach (Hyb‐Seq). Using this phylogeny along with other evidence, we propose a new taxonomic framework for the Crepidinae with seven lines and 29 genera, which merges subtribe Chondrillinae with the Crepidinae. We also describe a new monotypic genus, Qineryangia, that is characterized by broad involucres, loose imbricate phyllaries with wavy margins, and thick pappus bristles.
Phytochrome B photobody components
Authors: Kwon Yongmin , Kim Chanhee , Choi Giltsu 
Published: March 2024

SummaryPhytochrome B (phyB) is a red and far‐red photoreceptor that promotes light responses. Upon photoactivation, phyB enters the nucleus and forms a molecular condensate called a photobody through liquid–liquid phase separation. Phytochrome B photobody comprises phyB, the main scaffold molecule, and at least 37 client proteins. These clients belong to diverse functional categories enriched with transcription regulators, encompassing both positive and negative light signaling factors, with the functional bias toward the negative factors. The functionally diverse clients suggest that phyB photobody acts either as a trap to capture proteins, including negatively acting transcription regulators, for processes such as sequestration, modification, or degradation or as a hub where proteins are brought into close proximity for interaction in a light‐dependent manner.
Should left atrial appendage closure be considered in resistant left atrial appendage thrombus cases? ‘Former Foe, New Ally’
Authors: Ates Ahmet Hakan , Kivrak Ahmet , Karakulak Ugur Nadir , Coteli Cem , Yorgun Hikmet , Sahiner Mehmet Levent , Barıs Kaya Ergun , Aytemir Kudret 
Published: March 2024

AbstractBackgroundThe study explores left atrial appendage closure (LAAC) as a safe and effective alternative to anticoagulation for atrial fibrillation (AF) patients at high bleeding risk. Complications, such as cardioembolic events due to left atrial appendage thrombus (LAAT), highlight the need for alternative stroke prevention strategies.AimsThis research assesses LAAC's safety and efficacy in patients with LAAT, aiming to offer valuable insights into its potential as a viable option for stroke prevention in such cases.MethodsThe study included 205 patients who underwent LAAC using specific devices between September 2015 and February 2023. Among them, 32 patients had persistent LAAT. Baseline characteristics, antithrombotic medications, risk scores, and LAAC indications were documented. Patients were followed to monitor significant clinical events like stroke, cardiovascular mortality, and all‐cause mortality.ResultsThe mean age was 71.9 and mostly female. Indications for LAAC were ischemic cerebrovascular events (CVE) despite anticoagulation (25%), bleeding complications (major/minor, 37.5% each), or both. Successful LAA closure was achieved in all cases, with minimal pericardial effusion in one. One‐month follow‐up showed no major events or device‐related issues. Median follow‐up of 16.5 months saw 21.9% non‐cardiac deaths. The study underscores LAAC's efficacy for stroke prevention in patients with persistent LAAT.ConclusionsThe LAAC in cases of LAAT, whether pursued initially or as a deferred approach, demonstrates feasibility and safety, exhibiting notable procedural success and minimal incidence of periprocedural complications.
Effectiveness of a media literacy‐based smoking prevention program in female adolescents
Authors: Kim Sookyung , Lee Hyeonkyeong , Kim Sanghee , Lee Kyung Hee , Yoo Seunghyun , Hong Jin Eui 
Published: March 2024

AbstractObjectivesTo examine the effectiveness of a media literacy‐based smoking prevention program based on Ajzen's theory of planned behavior in female adolescents.MethodsThis quasi‐experimental study was conducted with female high school students aged 16–17 years in Seoul, Republic of Korea. The program provided eight sessions over 4 weeks. Quantitative data were collected before and after online surveys in an intervention (n = 21) and control (n = 21) groups, and analyzed using mixed analysis of variance. Qualitative data on participation experiences was collected by requesting the participants to answer open‐ended questions once a week during the intervention and performing co‐occurrence analysis of specific terms in the responses was conducted through text mining.ResultsAlthough the program decreased smoking intention and increased smoking media literacy in the intervention group, there were no significant differences between the groups. Qualitative results obtained from the intervention group showed cognitive and behavioral changes in the perception of the harmfulness of e‐cigarettes in the media and the expression of a willingness to overcome the temptation to smoke.ConclusionsOur findings show that the enhancement of smoking media literacy, specifically by correcting misconceptions regarding e‐cigarettes promoted by the new media, contributes smoking prevention in female adolescents. It supports calls for an expanded role of public health professionals in health education at the school level.
What explains elite affective polarization? Evidence from Canadian politicians
Authors: Lucas Jack , Sheffer Lior 
Published: March 2024

AbstractConcerns about affective polarization are on the rise globally, and it has been associated with negative outcomes such as diminished trust in government and discrimination against out‐partisans. While elected politicians are typically thought to be a major source of mass‐level affective polarization, and despite the policy and representation consequences of heightened partisan hostility in legislatures, existing research has focused almost exclusively on the measurement and explanation of affective polarization among citizens. As a result, we know far less about the magnitude and sources of elite affective polarization. Here, we take a step towards addressing this gap using an original survey of hundreds of Canadian local politicians, a setting uniquely situated for addressing the role of a host of individual‐level and institutional‐level predictors of affective polarization. We find that Canadian local politicians are, on average, less affectively polarized than the citizens they represent. However, levels of affective polarization among these politicians vary considerably, with higher levels of affective polarization among politicians who are ideologues, partisans, and who harbor strong progressive ambition. We conclude by discussing the implications of our findings for research on affective polarization and describe the need for comparative studies of affective polarization among political elites.
Fulvic acid alleviates the stress of low nitrogen on maize by promoting root development and nitrogen metabolism
Authors: Liang Yuanyuan , Wang Junbo , Wang Zeping , Hu Desheng , Jiang Ying , Han Yanlai , Wang Yi 
Published: March 2024

AbstractThe potential of fulvic acid (FA) to improve plant growth has been acknowledged, but its effect on plant growth and nutrient uptake under nutrient stress remains unclear. This study investigated the effects of different FA application rates on maize growth and nitrogen utilization under low nitrogen stress. The results showed that under low nitrogen stress, FA significantly stimulated maize growth, particularly root development, biomass, and nitrogen content. The enhanced activity levels of key enzymes in nitrogen metabolism were observed, along with differential gene expression in maize, which enriched nitrogen metabolism, amino acid metabolism and plant hormone metabolism. The application of FA regulated the hormones' level, reduced abscisic acid content in leaves and Me‐JA content in roots, and increased auxin and zeatin ribose content in leaves. This study concludes that, by promoting root development, nitrogen metabolism, and hormone metabolism, an appropriate concentration of FA can enhance plant tolerance to low nitrogen conditions and improve nitrogen use efficiency.
NRG2 family members of Arabidopsis and maize regulate nitrate signalling and promote nitrogen use efficiency
Authors: Li Shuna , Ji Meiling , Liu Fei , Zhu Mingyue , Yang Yi , Zhang Wenjing , Liu Shubing , Wang Yong , Lv Wei , Qi Shengdong 
Published: March 2024

AbstractNitrogen (N) is an essential nutrient for plant growth, and most plants absorb it as nitrate. AtNRG2 has been reported to play an important role in nitrate regulation. In this study, we investigated the functions of AtNRG2 family members of Arabidopsis thaliana and maize in nitrate signalling and metabolism. Our results showed that both AtNRG2.10 and AtNRG2.15 regulated nitrate signalling and metabolism. Overexpression of AtNRG2.11 (AtNRG2) could promote plant growth and improve nitrogen use efficiency (NUE). In addition, the maize genome harbors 23 ZmNRG2 members. We detected the expression of these genes treated with nitrate and the expression of four genes was strongly induced with ZmNRG2.7 having the highest levels. Overexpression of ZmNRG2.7 in the atnrg2 mutant could restore the defects of atnrg2, suggesting that ZmNRG2.7 is involved in nitrate signalling and metabolism. Moreover, the overexpression lines of ZmNRG2.7 showed increased biomass and NUE. These findings demonstrate that at least a part of NRG2 family genes in Arabidopsis and maize regulate nitrate signalling and provide a molecular basis for improving the NUE of crops.
Morphodynamics and depositional architecture of mid‐channel bars in large Amazonian rivers
Authors: Almeida Renato P. , Galeazzi Cristiano P. , Best Jim , Ianniruberto Marco , Do Prado Ariel H. , Janikian Liliane , Mazoca Carlos E. M. , Tamura Larissa N. , Nicholas Andrew 
Published: March 2024

AbstractLarge rivers are characterized by large water discharges, high suspended sediment fluxes and low slope, and typically display multiple channels that are separated by large complex bars. The most common channel style found in these rivers is characterized by the alternating presence of single and multiple channel threads. Mid‐channel compound bars separate a main deeper channel from shallower secondary channels, leading to low‐order braiding and low to moderate sinuosity. Despite the importance of this type of river for global terrestrial sediment transport and the interpretation of ancient fluvial successions, integrated depositional models for large multi‐channel rivers are still not fully developed. This paper interprets the channel morphodynamics and depositional architecture of such large rivers by investigating the distinctive features of their laterally‐accreting mid‐channel bars in the Solimões–Amazonas River. This is achieved by examination of temporal series of satellite images, quantification of bedforms using multibeam echosounding surveys, analysis of shallow seismic and ground penetrating radar surveys of selected areas, and on‐site field observations and sample collections. Such mid‐channel bars produce directional variability in planform scroll bar accretion of up to 180°. As these bars – and their associated channels – evolve, they become progressively shallower, due to the bifurcation of the two channels and the resultant partitioning of discharge that leads to a gradual reduction in transport capacity within the outer channel. The preserved successions of this process in the alluvial plain are characterized by fining‐upward trends, with larger cross‐strata sets at mid‐depths and internal erosional surfaces marking distinct cycles of bar development with potentially differing palaeocurrent trends. The new depositional models proposed for these mid‐channel bars and channels constitute a basis for the recognition of large multi‐channel river deposits in the ancient rock record.