bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sesquiterpenes of the ectomycorrhizal fungus Pisolithus microcarpus alter root growth and promote host colonization
Authors: Plett Jonathan M. , Wojtalewicz Dominika , Plett Krista L. , Collin Sabrina , Kohler Annegret , Jacob Christophe , Martin Francis 
Published: March 2024

AbstractTrees form symbioses with ectomycorrhizal (ECM) fungi, maintained in part through mutual benefit to both organisms. Our understanding of the signaling events leading to the successful interaction between the two partners requires further study. This is especially true for understanding the role of volatile signals produced by ECM fungi. Terpenoids are a predominant class of volatiles produced by ECM fungi. While several ECM genomes are enriched in the enzymes responsible for the production of these volatiles (i.e., terpene synthases (TPSs)) when compared to other fungi, we have limited understanding of the biochemical products associated with each enzyme and the physiological impact of specific terpenes on plant growth. Using a combination of phylogenetic analyses, RNA sequencing, and functional characterization of five TPSs from two distantly related ECM fungi (Laccaria bicolor and Pisolithus microcarpus), we investigated the role of these secondary metabolites during the establishment of symbiosis. We found that despite phylogenetic divergence, these TPSs produced very similar terpene profiles. We focused on the role of P. microcarpus terpenes and found that the fungus expressed a diverse array of mono-, di-, and sesquiterpenes prior to contact with the host. However, these metabolites were repressed following physical contact with the host Eucalyptus grandis. Exposure of E. grandis to heterologously produced terpenes (enriched primarily in $$\gamma$$
γ
-cadinene) led to a reduction in the root growth rate and an increase in P. microcarpus–colonized root tips. These results support a very early putative role of fungal-produced terpenes in the establishment of symbiosis between mycorrhizal fungi and their hosts.
Polydopamine functionalized dendritic fibrous silica nanoparticles as a generic platform for nucleic acid-based biosensing
Authors: Xue Xiaoting , Persson Helena , Ye Lei 
Published: March 2024

AbstractAccurate and rapid detection of nucleic acid sequences is of utmost importance in various fields, including disease monitoring, clinical treatment, gene analysis and drug discovery. In this study, we developed a "turn-on" fluorescence biosensor that enables simple and highly efficient detection of nucleic acid biomarkers. Our approach involves the utilization of 6-carboxyfluorescein modified single-stranded DNA (FAM-ssDNA) as molecular recognition element, along with polydopamine-functionalized dendritic fibrous nanosilica (DFNS). FAM-ssDNA serves as both specific molecular recognition element for the target analyte and reporter capable of transducing a detectable signal through Watson–Crick base pairing. The polydopamine-functionalized DFNS (DFNS@DA) exhibits strong binding to FAM-ssDNA via polyvalent metal mediated coordination leading to effective quenching by fluorescence resonance energy transfer. In the presence of a complementary target sequence, FAM-ssDNA forms hybridized structure and detaches from DFNS@DA, which causes an increased fluorescence emission. The analytical system based on FAM-ssDNA and DFNS@DA demonstrates exceptional sensitivity, selectivity, and rapid response for the detection of nucleic acid sequences, leveraging the high adsorption and quenching properties of DFNS@DA. For the first proof of concept, we demonstrated the successful detection of microRNA (miR-21) in cancer cells using the FAM-ssDNA/DFNS@DA system. Our results highlight the promising capabilities of DFNS@DA and nucleic acid-based biosensors, offering a generic and cost-effective solution for the detection of nucleic acid-related biomarkers.
Graphical abstract
Impedance changes of the Li-ion cell in the course of discharge
Authors: Křivík Petr 
Published: March 2024

AbstractThe paper deals with the measurement of the cell impedance parameters during discharging of the Li-ion NCR18650B cell. Re (Z) and Im (Z) of the battery were measured by PEIS method. The results of the impedance changes during discharging and charging were plot to Nyquist diagrams. The important values namely Rs, Rsei, Rct, Csei, Qsei, Cdl, α, and σ were found during discharging of the Li-ion cell with and without using CPE element.
Graphical abstract
Notfallkompetenzen für Medizinstudierende
Authors: Noll Christoph , Golon Sina , Kloppenburg Kira , Kluwe Lara-Sophie , Triefenbach Antonia , Behrends Marianne , Mücke Urs , Mikuteit Marie , Steffens Sandra 
Published: March 2024

Zusammenfassung
Hintergrund
Studierenden fehlt es in der Ausbildung noch an ausreichender Übungszeit zur Bildung von notfallmedizinischen Kompetenzen und klinischer Entscheidungsfindung [3]. Aspekte der Patient:innensicherheit und ökonomische Zwänge in einer Notaufnahme erschweren eine zielgerichtete kompetenzorientierte Lehre.

Fragestellung
Können mittels Virtual Reality (VR), eingebettet in einem Blended-Learning-Konzept, Studierenden der Humanmedizin die notwendigen Kompetenzen für die klinische Entscheidungsfindung in der Notfallmedizin vermittelt werden?

Material und Methoden
In einem Blended-Learning-Konzept mit verpflichtender Online-Vorbereitung und einer Peer-geführten VR-Simulation (Lerneinheit) in einer virtuellen Notaufnahme wurden Studierende in der Erstversorgung einer Notfallpatientin trainiert. Zusammen mit einer real-simulierten strukturierten Übergabe und anschließendem strukturiertem Feedback erfolgte eine Nachbereitung (Reflexion) der Lehrveranstaltung. Die Evaluierung der Lerneinheit erfolgte über einen Fragebogen zu den Punkten Simulation – Realität und Immersion, subjektive Kompetenzerweiterung und Vorbereitung zur Veranstaltung und über eine Befragung zur subjektiven Kompetenzeinschätzung vor und nach der Veranstaltung.

Ergebnisse und Diskussion
Im Rahmen eines curricularen Wahlpflichtangebots wurde unser Konzept im Studienjahr 2022/2023 von 40 % (n = 120) der Studierenden des 4. Studienjahrs an der Medizinischen Hochschule Hannover (MHH) genutzt. Das entwickelte Konzept ermöglicht es, Medizinstudierenden im 4. Studienjahr kompetenzorientiertes Notfallmanagement für Hochrisikosituationen zu vermitteln. Durch die Nutzung von VR können die Studierenden realistische Notfallszenarien in einer sicheren und kontrollierten Umgebung üben, ohne die Sicherheit der Patient:innen zu gefährden.

Graphic abstract
Whole-exome sequencing is feasible on a fresh-frozen skin sample of intravascular large B cell lymphoma
Authors: Bagnoli Filippo , Pini Giuditta , Ziccheddu Bachisio , Bonometti Arturo , Alberti-Violetti Silvia , Venegoni Luigia , Isimbaldi Giuseppe , Da Vià Matteo Claudio , Ferrari Angela , Baldini Luca , Neri Antonino , Onida Francesco , Bolli Niccolò , Berti Emilio 
Published: March 2024

AbstractIntravascular large B-cell lymphoma (IVLBCL) is a rare aggressive extranodal non-Hodgkin lymphoma. The predominant, if not exclusive, growth of neoplastic cells within the lumina of small-sized vessels represents the hallmark of the disease. Diagnosis is challenging due to the absence of marked lymphadenopathy, the highly heterogeneous clinical presentation, and the rarity of the condition. Clinical presentation is characterized by variable combinations of nonspecific signs and symptoms (such as fever and weight loss), organ-specific focal manifestations due to altered perfusion, and hemophagocytic syndrome. The rarity of this entity and the paucity of neoplastic cells in biopsy samples hamper the study of recurrent molecular abnormalities. The purpose of this study was to explore the feasibility of a different approach to recover a sufficient amount of DNA of acceptable quality to perform next-generation sequencing studies. Here, we report the findings of whole-exome next-generation sequencing performed on a fresh-frozen cutaneous sample of IVLBCL, paired with the patient saliva used as germline DNA. To increase the cancer cell fraction, only the subcutaneous tissue was selected. With this approach, we obtained high-quality DNA and were able to identify oncogenic mutations specific for this entity and recapitulating its post-germinal center origin, even if the tumor fraction was low. Molecular studies performed on fresh-frozen cutaneous sample are feasible in IVLBCL, especially when analysis is restricted to the subcutaneous tissue. Wide adoption of this reproducible and cost-effective approach may foster further studies, which may be of help in supporting diagnosis, providing pathogenetic insights, and guiding treatment decisions.
Computerized Segmentation Method for Nonmasses on Breast DCE-MRI Images Using ResUNet++ with Slice Sequence Learning and Cross-Phase Convolution
Authors: Hizukuri Akiyoshi , Nakayama Ryohei , Goto Mariko , Sakai Koji 
Published: March 2024

AbstractThe purpose of this study was to develop a computerized segmentation method for nonmasses using ResUNet++ with a slice sequence learning and cross-phase convolution to analyze temporal information in breast dynamic contrast material-enhanced magnetic resonance imaging (DCE-MRI) images. The dataset consisted of a series of DCE-MRI examinations from 54 patients, each containing three-phase images, which included one image that was acquired before contrast injection and two images that were acquired after contrast injection. In the proposed method, the region of interest (ROI) slice images are first extracted from each phase image. The slice images at the same position in each ROI are stacked to generate a three-dimensional (3D) tensor. A cross-phase convolution generates feature maps with the 3D tensor to incorporate the temporal information. Subsequently, the feature maps are used as the input layers for ResUNet++. New feature maps are extracted from the input data using the ResUNet++ encoders, following which the nonmass regions are segmented by a decoder. A convolutional long short-term memory layer is introduced into the decoder to analyze a sequence of slice images. When using the proposed method, the average detection accuracy of nonmasses, number of false positives, Jaccard coefficient, Dice similarity coefficient, positive predictive value, and sensitivity were 90.5%, 1.91, 0.563, 0.712, 0.714, and 0.727, respectively, larger than those obtained using 3D U-Net, V-Net, and nnFormer. The proposed method achieves high detection and shape accuracies and will be useful in differential diagnoses of nonmasses.
Promoting Reflection on the Process of Recovery: Unique Contributions from Literature and the Humanities for Practitioner
Authors: Lysaker Paul H. , Roe David , Lysaker John T. 
Published: March 2024

Abstract
Recovery from serious mental illness requires persons to make their own meaning and deal with evolving challenges and possibilities. Psychiatric rehabilitation thus must offer more than manualized curricula that address symptoms and skills. We suggest that exposure to the humanities and in particular literature may offer practitioners unique avenues for developing interventions that are sensitive to the processes that enable meaning to be made. We suggest that through what the poet Keats called negative capability, reading novels may enhance practitioners? abilities to see and accept uncertainty, tolerate ambiguity without need for complete resolution, and accept the complex and ambiguous nature of persons. As an illustration we described how reading two novels, The Trial and Slaughterhouse-Five enhanced the process of meaning making while supporting the recovery of one prototypical person with serious mental illness during his efforts to make sense of his experience of returning to work.
Crosstalk between vault RNAs and innate immunity
Authors: Avila-Bonilla Rodolfo Gamaliel , Martínez-Montero Juan Pablo 
Published: March 2024

Abstract

Purpose
Vault (vt) RNAs are noncoding (nc) RNAs transcribed by RNA polymerase III (RNA Pol III) with 5ʹ-triphosphate (5ʹ-PPP) termini that play significant roles and are recognized by innate immune sensors, including retinoic acid-inducible protein 1 (RIG-I). In addition, vtRNAs adopt secondary structures that can be targets of interferon-inducible protein kinase R (PKR) and the oligoadenylate synthetase (OAS)/RNase L system, both of which are important for activating antiviral defenses. However, changes in the expression of vtRNAs have been associated with pathological processes that activate proinflammatory pathways, which influence cellular events such as differentiation, aging, autophagy, apoptosis, and drug resistance in cancer cells.

Results
In this review, we summarized the biology of vtRNAs and focused on their interactions with the innate immune system. These findings provide insights into the diverse roles of vtRNAs and their correlation with various cellular processes to improve our understanding of their biological functions.
Development and Factor Structure of Problematic Multidimensional Smartphone Use Scale
Authors: Geçer Ekmel , Yıldırım Murat , Bağci Hakkı , Atar Cihat 
Published: March 2024

AbstractLiving in an intensifying technological and digital environment makes people more engaged with smartphones and related internet platforms. Alongside its highly debated advantages and disadvantages, modern smartphone use is a global phenomenon which has been mainly debated within the context of cultural and technology relationships which also influence cross-cultural activities. Focusing on one of the modern factors (i.e., smartphone use) that impact human behavior, this article presents the development of a measure of the Multidimensional Smartphone Use Scale (MSUS) and tests its factor structure to offer a scale that covers smartphone-related problems from a broader perspective. Using an online survey, a total of 514 participants took part in the current study. The scale was developed to measure a multidimensional conception of smartphone use-related problems and behavioral disorders including nomophobia, ringxiety, textiety, and phubbing which were supported with exploratory and confirmatory factors analyses. The MSUS indicated good internal consistency reliability ranging from α = .75 to α = .89. This article presents the preliminary evidence regarding the reliability and validity of the MSUS which can be used in a wide range of settings to measure maladaptive modes of smartphone use and related behaviors.
International constitutional advising: Introducing a new dataset
Authors: Lerner Hanna , Pereira David Futscher , Schlager Nina 
Published: March 2024

AbstractThe article presents the first systematic comparative study on the growing involvement of international organizations in national constitution making around the world. Over the past three decades, the emerging field of international constitutional advising has undergone an intense process of institutionalization and professionalization, mirroring the increasing role constitution making is playing in both national and international politics. Despite the vast scope of the phenomenon, the involvement of foreign constitutional advisors in domestic constitution-drafting or constitutional reforms has received little scholarly attention. This article takes the first steps towards addressing this lacuna empirically, by introducing a new dataset on 46 international organizations involved in 730 constitutional advising projects in 145 countries between 1989 and 2017. We classified the organizations based on their type, their headquarters’ location, the countries they target, the kind of advising activities they perform and the level of directness of the advising intervention. While generally, we find a significant correlation between more direct constitutional advising activities and larger relative changes in the quality of democracy and larger numbers of constitutional systems in a country, the article suggests avenues for more nuanced research to better understand constitutional advising’s impact.
On the relation between affinely adjustable robust linear complementarity and mixed-integer linear feasibility problems
Authors: Biefel Christian , Schmidt Martin 
Published: March 2024

AbstractWe consider adjustable robust linear complementarity problems and extend the results of Biefel et al. (SIAM J Optim 32:152–172, 2022) towards convex and compact uncertainty sets. Moreover, for the case of polyhedral uncertainty sets, we prove that computing an adjustable robust solution of a given linear complementarity problem is equivalent to solving a properly chosen mixed-integer linear feasibility problem.
Experimental Construction and Validation of Revised Drucker–Prager Model Using Finite Element Method for Moisture Condensation Zone in Bentonite-Bonded Silica Sand
Authors: Okimura Yasuhiko , Imamura Rei , Shimo Kohei , Hanai Takashi , Kato Yusuke , Hashimoto Kunihiro , Faiz Muhammad Khairi , Okane Toshimitsu , Miyashita Tomoyuki , Yoshida Makoto 
Published: March 2024

AbstractFor predicting casting deformation by FEM (finite element method) thermal stress analysis, this paper experimentally constructed and validated the Drucker–Prager (DP) model and its revised model (RDP) for the condensation zone of bentonite-bonded sand molds. The condensation zone has been known as a remarkably low-strength region in the mold, and its mechanical response should be dominant to the casting deformation. To construct the models through uniaxial and triaxial compression tests, the test pieces reproducing the condensation zone were prepared by permeating water vapor through a test piece of bentonite-bonded sand. The constructed models were then applied to the FEM stress analysis of the triaxial test to validate them by comparing the analytical stress–strain curves with the experiment. The experimental stress–strain curve after permeating water did not show any distinct yielding point; however, the analytical curve with the original DP model clearly exhibited a yielding point due to the yield criterion of the model. The distinct yielding criterion should be essentially fatal to reproduce the stress–strain relationship of the condensation zone. Contrarily, the RDP model reproduced the experimental curve with less than 10 % error and was found to be advantageous for modeling the smooth stress–strain relationship of the condensation zone.
Multilateralism and Soft Power Made-in-China: (re)Adjusting Role Conception to Meet International Expectations
Authors: Duarte Paulo Afonso Brardo , Gupta Amit , Delvaje Bruna Cristina 
Published: March 2024

AbstractThis article addresses the specificities of the new multilateralism made-in-China under Xi Jinping. We argue that China has been investing in a combination of Soft Power and Multilateralism to foster a friendly worldwide environment whilst promoting China’s geopolitical reemergence. Drawing on role theory, we assess whether there has been a shifting trend on China’s soft power and multilateralism, to cope both with international expectations on China’s new role and China’s own role conception. We conclude that China’s gradual turn towards multilateralism and soft power is a complementary strategy to China’s longstanding use of bilateralism. It provides China with new institutions and ways to prosper as Chinese interests are no longer effectively fulfilled within the old Bretton Woods system. This article aims to deepen the existing literature on China’s soft power, whilst highlighting the novel developments in China’s multilateral initiatives and soft power including the impact of EU’s de-risking approach toward China – not yet addressed by current studies.
The impact of radiomics in the management of soft tissue sarcoma
Authors: De Angelis Riccardo , Casale Roberto , Coquelet Nicolas , Ikhlef Samia , Mokhtari Ayoub , Simoni Paolo , Bali Maria Antonietta 
Published: March 2024

Abstract
Introduction
Soft tissue sarcomas (STSs) are rare malignancies. Pre-therapeutic tumour grading and assessment are crucial in making treatment decisions. Radiomics is a high-throughput method for analysing imaging data, providing quantitative information beyond expert assessment. This review highlights the role of radiomic texture analysis in STSs evaluation.

Materials and methods
We conducted a systematic review according to the Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A comprehensive search was conducted in PubMed/MEDLINE and Scopus using the search terms: ‘radiomics [All Fields] AND ("soft tissue sarcoma" [All Fields] OR "soft tissue sarcomas" [All Fields])’. Only original articles, referring to humans, were included.

Results
A preliminary search conducted on PubMed/MEDLINE and Scopus provided 74 and 93 studies respectively. Based on the previously described criteria, 49 papers were selected, with a publication range from July 2015 to June 2023. The main domains of interest were risk stratification, histological grading prediction, technical feasibility/reproductive aspects, treatment response.

Conclusions
With an increasing interest over the last years, the use of radiomics appears to have potential for assessing STSs from initial diagnosis to predicting treatment response. However, additional and extensive research is necessary to validate the effectiveness of radiomics parameters and to integrate them into a comprehensive decision support system.
A new possible marker: can pennation angle defined by ultrasound predict the frailty?
Authors: Yurumez Busra , Metin Yavuz , Atmis Volkan , Karadavut Mursel , Ari Sinan , Gemci Emine , Yigit Seher , Ozalp Ates Funda Seher , Gozukara Melih Gaffar , Kaplankiran Ceren , Cosarderelioglu Caglar , Yalcin Ahmet , Aras Sevgi , Varli Murat 
Published: March 2024

Abstract
Background
Frailty indicates older people who are vulnerable to stressors. The relation between ultrasonographic parameters of muscle and frailty among older people has yet to be investigated.

Aims
The aim of the study is to investigate the relationship between frailty and the ultrasonographic measurements of the rectus femoris muscle (RFM).

Methods
This cross-sectional study included 301 participants who were ≥65 years. The FRAIL questionnaire assessed frailty. The thickness, cross-sectional area (CSA), fascicle length, pennation angle (PA), stiffness, and echogenicity of RFM were assessed by ultrasound. The accuracy of parameters in predicting the frailty was evaluated by ROC analysis.

Results
Of all 301 participants, 24.6% were frail. Pre-frail and frail participants had significantly lower thickness (p = 0.002), CSA (p = 0.009), and fascicle length (p = 0.043) of RFM compared to robust. PA was significantly lowest in frails (p < 0.001). The multivariate logistic regression analysis showed that PA values lower than 10.65 degrees were an independent predictor of frailty (OR = 0.83, 95% Cl: 0.70–0.97, p = 0.019). Results of ROC analysis demonstrated a satisfactory result between the PA and frailty (AUC = 0.692, p < 0.001).

Discussion
Thickness, CSA, and PA of RFM were found to be lower in frail subjects, which may indicate the changes in muscle structure in frailty. Among all parameters, lower PA values were independent predictors of frailty. These findings may indicate a novel ultrasound-based method in frailty, that is more objective and unrelated to the cross-sectional evaluation.

Conclusions
Ultrasonographic measurements of RFM, especially the lower PA may predict frailty in older people. As an objective and quantitative method, PA may be used to define frailty with acceptable sensitivity.