bluuebunny/crossref_metadata_2025_split huggingface dataset:
A MARMOSET T‐LYMPHOCYTE PROTEIN RELATED TO DEFINED HUMAN SERUM IMMUNOGLOBULIN AND FRAGMENTS
Authors: Marchalonis J. J. , Wang A. C. 
Published: April 2007

SummaryCurrent concepts of the antigen‐specific receptor of thymus‐derived (T) lymphocytes suggest that this molecule bears immunoglobulin (Ig) variable (V) regions, but does not represent a classical serum antibody. We immunized rabbits with a globulin fraction obtained from an in vitro cultured marmoset T cell lymphoma line which expressed surface components related to immunoglobulins. Quantitative immunoprecipitation and competition radioimmunoassay studies carried out using characterized monoclonal human Igs and their chains and fragments show that this rabbit antiserum, raised aginst a T cell product, reacts with a particular, conformational VH‐determinant which is formed by interaction with λ light chains and with two μ chain (IgM) related determinants. Neither μ chain determinant is a major isotype specific marker, and each occurs only on certain distinct subsets of serum μ chains. One is located in the Fc fragment; the location of the other μ related determinant is not known. These results show that this rabbit antiserum recognizes both variable and constant region determinants on the T cell receptor heavy chain. Rabbit antibody to the T cell product which cross‐reacts with serum μ chain was isolated by immune affinity chromatography on Sepharose‐derivatized covalently with one particular reactive IgM protein, the Waldenstrom macroglobulin Can (kμ). This antibody precipitated a biosynthetically‐labelled component of approximate M. W. 70,000 as assessed by polyacrylamide gel electrophoresis in Na Dod SO4‐containing buffers. These data support the conclusion that certain T cells express and synthesize a molecule related to Ig VH‐regions which also has a constant region sharing antigenic markers with IgM subpopulations.
GENETIC CONTROL OF ANTINUCLEAR ANTIBODIES IN MICE INOCULATED WITH THE MOLONEY LEUKAEMIA VIRUS
Authors: Piatier D. , Debre P. , Mach P. S. , Gisselbrecht S. 
Published: April 2007

SummaryThe production of antinuclear antibodies (ANA) was studied after inoculation with Moloney leukaemia virus (M‐MuLV) in different H‐2 congenic strains of mice. Using a new sensitive method for ANA detection, it was demonstrated that M‐MuLV‐induced ANA were genetically controlled by several different factors. A high viral production was first required for ANA triggering. Among viremic animals both high and low ANA producers were observed. H‐2 and non‐H‐2‐linked genes were involved in the control of M‐MuLV‐induced ANA; these genes were different from those involved in the control of viremia. The H‐2b haplotype was associated with an increased ANA response, the transmission of the responder phenotype being intermediate. Non‐H‐2‐linked genes must also control M‐MuLV‐induced ANA, as demonstrated in mice having the same H‐2 haplotypes, since with equivalent viremias they produced different amounts of ANA. No linkage with X chromosome was found.
THE B REGION‐ASSOCIATED ANTIGENS AND MLR PHENOTYPES IN THE JAPANESE INBRED STRAINS OF RATS
Authors: Ohhashi T. , Iwabuchi K. , Natori T. , Nakagawa H. , Kikuchi Y. , Aizawa M. 
Published: April 2007

SummarySeven different alloantisera absorbed with red blood cells (RBC) from appropriate strains of rats detected a series of B cell alloantigenic specificities that could be divided into two groups, presumably coded for by at least two different closely‐linked loci in the rat major histocompatibility complex (MHC), RT1. The one locus had two allele codes for a broad specificity and the other locus codes for a unique specificity that was found only in the restricted strains of rats that shared the same mixed lymphocyte reaction (MLR) phenotype. RBC‐absorbed alloantisera were monitored against a panel of B cell fractions obtained from sixteen inbred strains. Two alloantisera, ACI anti‐W and W anti‐TO detected two broad specificities, into either of which all inbred strains tested were classified. Two broad RT1‐B region‐associated specificities were thus designated provisionally as Ba‐1.1 and ‐1.2. Another five alloantisera detected four respective specificities which have a narrower strain distribution. Sixteen inbred strains were classified into one of five specificities detected by W anti‐F344, F344 anti‐SDJ, WKA anti‐ACI, W anti‐BUF and ACI anti‐W absorbed with LEJ lymph node cells. Each specificity was designated provisionally as Ba‐2.1, ‐2.2, ‐2.4, ‐2.6, ‐2.7, respectively. A complete association of Ba‐2 specificities with MLR phenotype was observed. Antigenic specificities of Ba‐2.1, ‐2.2 and ‐2.4 were all classified in a group of Ba‐1.1 specificity, whereas Ba‐2.6 and ‐2.7 specificities were associated with Ba‐1.2 specificity. This relationship suggested a linkage disequilibrium between the two loci for the Ba antigens.
AN AMPHIOXUS LANCEOLATUS AGGLUTINATING FACTOR FOR HUMAN RED CELLS
Authors: Benedictis Giovanna De , Capalbo P. 
Published: April 2007

SummaryHumoral proteins from Amphioxus lanceolatus showed a considerable agglutinating activity for human red cells. The haemagglutinating factor eluted in the macroglobulin peak on Sephadex G200 and resulted ConA‐ at affinity chromatography on Sepharose ConA. Immunoelectrophoresis, carried out using a specific antiserum produced in rabbit, revealed α mobility for the agglutinating factor, thus suggesting that it was an α macroglobulin.
SHORT COMMUNICATION: EVIDENCE FOR AN H‐2‐LINKED Ir GENE REGULATING THE IMMUNE RESPONSE TO A TI‐2 ANTIGEN, DNP‐FICOLL
Authors: Schott C. F. , Lizzio Elaine F. , Merchant B. 
Published: April 2007

SummaryVarious H‐2 congenic mouse pairs were immunized with 10 μg of DNP53‐Ficoll, a thymic independent (TI‐2) antigen. Four‐day primary splenic immune responses were measured by a DNP‐reactive PFC assay. Significantly different responses were noted between partners of many congenic pairs. An H‐2‐linked Ir gene for DNP‐Ficoll appears likely.
INTERSPECIFIC DISTRIBUTION OF ALLOTYPIC MINK (MUSTELA VISION) IgG ANTIGENS
Authors: Baranov O. K. , Fomicheva I. I. , Ternovsky D. V. , Ternovskaya J. G. 
Published: April 2007

SummaryThe antigenic specificities of six (1‐6) IgG allotypes of the domestic mink were tested in the sera of closely related species of Mustelidae family and distant mammalian species. It was found that allotypes 1 and 5 are ancient. Their antigenic specificities were established not only in Mustelidae, but also in other taxonomic orders of mammals. Allotypes 3 and 2 are phylogenetically younger; they were detected only in Mustelidae. Allotypes 4 and 6 appear to be unique to the domestic mink.The instantaneous evolutionary emergence of complex allotypes 4 and 6 is difficult to explain by a rapid accumulation of gene point mutations during phylogenesis. Activation in the domestic mink of those immunoglobulin genes, which are silent or poorly expressed in closely related Mustelidae, is suggested as a more plausible explanation.
THE OCCURRENCE OF THOMSEN‐ FRIEDENREICH RECEPTORS IN BOVINE ERYTHROCYTES OF VARIOUS FV GENOTYPES
Authors: Sellei J. 
Published: April 2007

SummaryIn the V/V type of bovine erythrocytes (BE), the antigen IM and the Thomsen‐Friedenreich (TF) receptors, which reacted with a TF‐specific agglutinin from human sera and the lectin from Helix pomatia, were all destroyed by treatment with trypsin. Unlike the V/V, in the F/F type of BE the antigen IM and these TF receptors were not only not destroyed by trypsin, but this enzyme treatment was, in fact, necessary to make them amenable to the corresponding agglutinins.Another TF receptor, which reacted with the lectin from peanuts, was identified on a trypsin‐resistant component of all BE and independently of the FV genotypes.
COMPOSITION AND IMMUNOCHEMICAL PROPERTIES OF GLYCOPROTEINS WITH ANTI‐B AGGLUTININ ACTIVITY ISOLATED FROM EUONYMUS SIEBOLDIANA SEEDS
Authors: Yamamoto S. , Sakai I. 
Published: April 2007

SummaryFive major glycoproteins with anti‐B agglutinin activity were isolated from seeds of Euonymus Sieboldiana by a procedure based on precipitation with ammonium sulphate, Sepharose 4B gel filtration, CM‐ and DEAE‐Sepharose chromatography and Sephacryl S‐200 gel filtration. The purified glycoproteins each gave a single symmetrical peak on Sephacryl S‐200 gel filtration with elution volumes corresponding to molecular weights of approximately 15,000 to 130,000, each forming a single precipitin line on gel diffusion plates with anti‐E. Sieboldiana antibody. These anti‐B glycoproteins were rich in acidic amino acids without cysteine and methionine and contained about 8‐36% carbohydrate, of which galactose, arabinose and glucose were the predominant sugars, with small amounts of glucosamine, rhamnose, fucose, xylose, mannose and ribose. The most anti‐B active lectin agglutinated human B red blood cells at a concentration of 2 μg/ml and was strongly inhibited by melibiose. The three other lectins with anti‐B agglutinin activity, however, were not inhibited by any galactose‐containing glycosides.
PERSISTENCE OF CYTOTOXIC ANTIBODIES TO HLA‐A,B,C ANTIGENS AND TO Ia‐LIKE ANTIGENS IN PAROUS WOMEN
Authors: Curtoni E. S. , Pellegrino M. A. , Indiveri F. , Borelli I. , Ferrone S. 
Published: April 2007

SummaryCytotoxic antibodies to HLA‐A,B,C and to Ia‐like antigens were detected in about 8% of sera drawn from 1312 women several years after their last pregnancy. In the majority of sera anti Ia‐like antigen antibodies were not associated with anti HLA‐A,B,C antibodies. Persistence of both types of antibodies was not correlated with the number of pregnancies and with the time interval between the last immunizing stimulus and the drawing of the sample. Testing of the sera with a panel of HLA typed lymphocytes identified 20 sera specific for HLA‐A,B alloantigens and 7 specific for Ia‐like alloantigens.
THE ROLE OF H‐2 I REGION GENES IN REGULATION OF THE IMMUNE RESPONSE *
Authors: McDevitt H. O. 
Published: April 2007

SummaryThe evidence of the past 10 years indicates that genes mapping in the I region of the mouse major histocompatibility complex (H‐2) regulate a bewildering array of immunologic functions. A survey of H‐2‐linked specific immune response (Ir) genes shows that the phenotypic effect of these genes is to enable a particular inbred strain to discriminate and recognize remarkably precise chemical specificities, such as one or two amino acid interchanges in a polypeptide chain. The only I region gene products which have been identified to date are the Ia antigens. These include five readily detectable polypeptide chains (Aa, Ab, Ae, Eα, and Ii) and several other serologically distinct entities which are selectively expressed on functionally distinct T cell subsets (J1, J2?, J3? and C). The specificity of recognition of Ir genes would seem to require a larger number of I region gene products than can be generated even by combinatorial association of the four readily identifiable peptides (to give eight combinatorial associations) and the other serologically identified gene products. If the Ia antigens are to function as an antigen specific receptor system, separate from immunoglobulin molecules, there must be other, as yet undetected, I region gene products (e.g. Ia antigens). Alternatively, the known I region gene products could function by any one of several postulated mechanisms to generate an antigen specific T cell receptor system. The available evidence for the total number of I region gene products is reviewed, and the alternate possibilities are briefly discussed in this presentation.
COMPARISON OF THE HAPLOTYPES OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN THE RAT: V. A SEROLOGICAL, GENETIC AND NUMERICAL STUDY OF THE RT1.A REGION ANTIGENS
Authors: Arenas O. , Wagener D. K. , Kunz H. W. , Gill T. J. 
Published: April 2007

SummaryThe classification of the RT1.A phenotypes of a large number of inbred and congenic strains of rats, including those prototypic for all of the known RT1.A phenotypes, was done by examining the reactivity of a variety of unabsorbed antisera with cells of the different strains. The data were analysed by the techniques of numerical taxonomy without reference to the previous classification by the classical serological methods. The most effective way of analysing the data was the use of the Jaccard correlation coefficient and the average linkage method of statistical analysis. Four conclusions were drawn from this study. First, the RT2 antigens must be matched in the donor and recipient pair used to raise the anti‐RT1 (MHC) antiserum or spurious typing results can occur due to the presence of anti‐RT2 antibodies. Second, using anti‐RT1 antisera raised in RT2 compatible pairs of rats, the classification of inbred and congenic strains into the RT1.A phenotypes on the basis of classical serological studies was confirmed. Third, two groups of closely related RT1.Ac phenytopes were found (a, f, d and b, m). Fourth, the AUG and PVG strains, both of which carry the the RT1.Ac phenotype, are quite distantly related on the basis of this taxonomic analysis and the RTl.Ag and RT1.An phenotypes are the most distantly related compared to all of the other rat strains and to each other. These data provide further evidence that the RT1 polymorphism of the rat is very limited, especially in respect of man and mouse.
EVIDENCE FOR EXPRESSION OF Ly ‐ 6.2 ON NON‐BONE MARROW‐DERIVED CELLS IN KIDNEY, SKIN AND CONNECTIVE TISSUES
Authors: HALLORAN P. F. , RICHARDS R. R. , STUBBS MARLENE , GIDON M. 
Published: April 2007

SummaryMouse alloantigen Ly‐6.2 is detectable in various non‐lymphoid tissues such as kidney, but it is not clear whether or not this expression is due to bone‐marrow derived passenger leukocytes. To determine whether non‐marrow derived cells express Ly‐6.2, we examined the expression of this antigen in kidney and on isolated connective tissue and epidermal cells. Studies in radiation chimeras demonstrated that the kidney did not become Ly‐6.2 positive when negative animals were reconstituted with positive marrow. Thus, passenger leukocytes cannot account for the renal expression of Ly‐6.2, indicating that most of this antigen is on non‐marrow‐derived (parenchymal) cells in kidney. Various isolated cell types‐fibroblasts, osteocytes, chondrocytes and skin epidermal cells‐were found to be Ly‐6.2 positive. Indeed, absorption and cytotoxicity results suggested that the amount of Ly‐6.2 on fibroblasts exceeded the amount of an H‐2 antigen on these cells. Comparison of fibroblast to lymphocytes indicated that fibroblastts had 13‐60 times more Ly‐6.2 than spleen cells and three times more than PHA blasts. The results indicate that the Ly‐6.2 detected in non‐lymphoid tissues is predominantly on the parenchymal or connective tissue elements of those tissues.
SERUM IMMUNOGLOBULINS AND LYMPHOCYTE SUBPOPULATIONS DERANGEMENT IN TURNER'S SYNDROME
Authors: Cacciari E. , Masi M. , Fantini Maria Pia , Licastro F. , Cicognani A. , Pirazzoli P. , Villa Maria Pia , Specchia F. , Forabosco A. , Franceschi C. , Martoni L. 
Published: April 2007

SUMMARYAbnormalities of the proportions of peripheral blood lymphocyte subpopulations and of immunoglobulin serum levels were found in twenty patients affected by Turner's syndrome. A slight but significantly decreased percentage of circulating T and B cells, an increased percentage of null cells and a decreased in vitro, responsiveness of lymphocytes to phytohaemagglutinin, concanavalin A and pokeweed mitogen were found in Turner's syndrome patients. IgG serum level was found significantly decreased in comparison with age‐matched fifty‐seven normal males and fifty‐seven normal females and IgM serum level was intermediate between female and male values; Turner's syndrome patients with monosomy had an IgM serum concentration very close to male values. The derangement of T and B lymphocyte subpopulations, probably related to the aneuploidy, does not seem to be a severe one but it could account for the immunoglobulin abnormalities and for the association of Turner's syndrome with immunological disorders such as autoimmune diseases. The role of X chromosome on IgM serum level is discussed.
GENETIC CONTROL OF CELL‐MEDIATED IMMUNITY IN THE RAT. I. TRANSFER OF DTH TO BACTERIAL ANTIGENS IS RESTRICTED BY THE B REGION OF RT1.
Authors: Jungi T. W. , Kunz H. W. , Gill, T. J. 
Published: April 2007

SUMMARYThe genetic basis of allogeneic restriction in the transfer of delayed type hypersensitivity (DTH) to soluble Listeria monocytogenes antigens (LMA) in rats was analysed using a set of congenic strains. The DTH parameter assayed was the localization of radiolabelled donor lymphoblasts in subcutaneous antigen stimulation sites in the ear. Sharing of the RT1.B region between the donor and host was essential for the transfer of DTH to LMA, which suggests that the RT1.B region codes for restriction elements controlling antigen recognition by TDTH cells. The recombinant haplotype RT1r1 was exceptional in that transfer of DTH to A‐region matched recipients was, at least in part, possible. Measurement of donor lymphoblast localization in DTH sites afforded an opportunity to quantify allogeneic effects influencing localization of sensitized donor cells in DTH sites borne by alien recipients. Both RT1.B and RT1.A region differences could induce allogeneic effects, but they were an order of magnitude lower than those based on restricted recognition by donor T cells.
IMMUNOGLOBULIN ALLOTYPES IN FILIPINOS: I. LACK OF ASSOCIATION BETWEEN SCHISTOSOMA JAPONICA INFECTION AND IMMUNOGLOBULIN ALLOTYPES
Authors: Yogore M. G. , Schanfield M. S. 
Published: April 2007

SUMMARYAn attempt to determine association between immunoglobulin allotypes and Schistosoma japonica infection was made. The results show that no apparent relationship exists.
GENETIC CONTROL OF SERUM IMMUNOGLOBULIN G LEVELS IN THE CHICKEN
Authors: Rees M. J. , Nordskog A. W. 
Published: April 2007

SUMMARYSerum IgG (7S) levels differed significantly for chickens from 10 different inbred lines. Within lines differences between B blood groups were statistically significant.The genetic control of serum IgG was further examined using birds from B complex haplotypes marked at the B locus and the Ir‐GAT locus. Birds from each of five subgroup haplotypes (B1B1Ir‐GAT‐Lo and ‐Hi, B19B19Ir‐GAT‐Lo and ‐Hi, and B2B2Ir‐GAT intermediate) were tested for levels of serum IgG at 3, 6, 9, and 21 weeks of age. The rate and level of IgG reached in the serum was more than two‐fold greater in the GAT‐Hi birds than in the GAT‐Lo. The Ir region of the B complex exerts some control over the ontogenesis of IgG, though most of the genetic variation seems not to be B complex associated.
REACTIVITY OF MONOCLONAL ANTIBODIES AGAINST HUMAN LEUCOCYTE ANTIGENS WITH LYMPHOCYTES OF NON‐HUMAN PRIMATE ORIGIN
Authors: Neubauer R. H. , Levy* R. , Strnad B. C. , Rabin H. 
Published: April 2007

SUMMARYThe phylogenetic distribution of antigens present on human lymphocytes was investigated by incubating human or simian cells with murine anti‐human monoclonal antibodies and then determining the level of reactivity with a radiolabelled anti‐murine IgG reagent. The monoclonal antibodies used were specific for a T‐cell antigen, lymphoid and lymphoid:myeloid antigens, Ia antigens, and β2 microglobulin. The cells examined included B‐ and T‐lymphoblastoid cell lines and fresh peripheral blood lymphocytes separated by sheep erythrocyte rosetting into T‐cell and non T‐cell fractions. Results of these studies showed that the antibodies gave complete cross‐reactivity with gorilla and chimpanzee cells while B‐cell lines of orangutan origin had lost lymphoid and β2 microglobulin markers. Gibbon cells and cells of Old World and New World monkeys reacted strongly only with monoclonal antibodies against Ia antigenic determinants. These Ia antigens were found on the non T‐cell fraction of fresh peripheral lymphocytes, on B‐cell lines and on some virus induced T‐cell tumour lines. Immunoprecipitation analysis using the anti‐Ia antibodies showed a degree of molecular diversity on owl monkey and marmoset cells compared to the Ia antigens associated with human cells.
VAGINAL SEPTA FREQUENCY INFLUENCED BY MAJOR HISTOCOMPATIBILITY COMPLEX, H‐2
Authors: Bonner J. J. 
Published: April 2007

SUMMARYDorso‐ventral vaginal septa were observed in congenic mouse strains. Strain C57BL/10Sn, H‐2b, had the highest frequency, 22%; B10.A/SgSn, H‐2a, had the lowest frequency, 4%, P <0.001. The frequency was about 17% in the F1, females. Septa were found in 16% and 15% of the B10.D2/nSn and B10.BR/SgSn strains, respectively, indicating that at least two loci within the H‐2 complex influence its frequency.
GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAEMOGLOBIN: III. VARIANT Aβ (bm 12) BUT NOT Ae (D2.GD) Ia POLYPEPTIDES ALTER IMMUNE RESPONSIVENESS TOWARDS THE α‐SUBUNIT OF HUMAN HAEMOGLOBIN
Authors: Krco C. J. , Kazim A. L. , Atassi M. Z. , And R. Melvold , David C. S. 
Published: April 2007

SUMMARYMice bearing the I‐A subregion mutation bm12 were immunized and challenged with the α‐subunit of human adult haemoglobin. Under conditions in which parental B6/Kh mice respond, B6.C‐H‐2bm12 mice are inhibited nearly 100% in their ability to respond to challenge to the α‐chain of haemoglobin. D2.GD mice which express a variant Ae (Eβ) polypeptide of the I‐E subregion can respond as well as B10.D2 mice to both subunits (α‐ and β‐) of haemoglobin. These observations as well as other genetic mapping data confirm the I‐A mapping of α‐chain‐specific Ir genes and extend the genetic fine mapping to the Aβ gene within the I‐A subregion or a combinatorial Ia determinant generated by an interaction of Aα and Aβ. In addition they implicate the Ia.8 specificity in determining immune responsiveness to α‐chain determinants.
DEFINITION OF ALIEN H‐2 DETERMINANTS ON A FRIEND LEUKAEMIA BY ANALYSIS OF ALLOREACTIVITY OF CTL FROM PRIMARY MLC
Authors: Greenberg P. D. , Cheever M. A. , Fefer A. 
Published: April 2007

SUMMARYA Friend virus‐induced tumour of BALB/c (H‐2d) origin, HFL/d, was examined for the expression of alien H‐2 antigens. The alloantigens on HFL/d were typed by generating CTL in primary MLC with HFL/d as stimulator and measuring reactivity to targets with known H‐2 antigens, and confirmed by assessing recognition of HFL/d targets by CTL generated in primary MLC with stimulators expressing known H‐2 antigens. Potential cross‐reactivities between alloantigens were analysed by cold‐target inhibition experiments. BALB/c cells stimulated with HFL/d lysed H‐2b targets, and BALB/c anti‐H‐2b CTL lysed HFL/d; analysis with recombinant haplotypes demonstrated both H‐2Kb and H‐2Db alien antigens on HFL/d. C57BL/6 (H‐2b) cells stimulated with HFL/d recognized H‐2Kd, H‐2Dd, and an additional determinant unique to HFL/d. (BALB/c x B6)F1 cells also recognised a unique HFL/d determinant not of H‐2b or H‐2d origin. These unique determinants, which induced a strong cytotoxic response in primary MLC, were not shared by BALB/c or B6 tumours induced by cross‐reactive FMR viruses. Thus, HFL/d expressed the K and D antigens of its strain of origin, two typed alien H‐2 antigens, and at least one other untyped antigen which may represent an additional H‐2 determinant. These studies further demonstrate the utility of examining the reactivity of CTL generated in primary MLC to probe for the presence of alien H‐2 antigens.
PROPERDIN FACTOR B POLYMORPHISM IN BLACK AMERICANS
Authors: Budowle B. , Go R. C. P. , Barger B. O. , Acton R. T. 
Published: April 2007

SUMMARYProperdin factor B is a genetically‐controlled polymorphism that can be demonstrated by agarose gel electrophoresis. The Bf gene frequencies were determined for 194 blacks from the south‐eastern United States. The frequencies for Bf‐F, Bf‐S, Bf‐F1, and Bf‐Sl are 0.626, 0.327, 0.034, and 0.013, respectively. The south‐eastern blacks have similar Bf frequencies to the South African blacks; however, both these groups are different from blacks from Boston. The frequency differences between south‐eastern blacks and Boston blacks may be due to racial admixture differences found in the two populations.