bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactucopicrin promotes fatty acid β‐oxidation and attenuates lipid accumulation through adenosine monophosphate‐activated protein kinase activation in free fatty acid‐induced human hepatoblastoma cancer cells
Authors: Tan Huiwen , Mi Na , Tong Fenglian , Zhang Rui , Abudurexiti Adalaiti , Lei Yi , Zhong Yewei , Yan Junlin , Yang Jian , Ma Xiaoli 
Published: April 2024

AbstractWith its annually increasing prevalence, non‐alcoholic fatty liver disease (NAFLD) has become a serious threat to people's life and health. After a preliminary research, we found that Lactucopicrin has pharmacological effects, such as lowering blood lipids and protecting the liver. Further research showed its significant activation for fatty acid β‐oxidase hydroxyacyl‐coenzyme A (CoA) dehydrogenase trifunctional multienzyme complex subunit alpha (HADHA), so we hypothesized that Lactucopicrin could ameliorate lipid accumulation in hepatocytes by promoting fatty acid β‐oxidation. In this study, free fatty acid (FFA)‐induced human hepatoblastoma cancer cells (HepG2) were used to establish an in vitro NAFLD model to investigate the molecular basis of Lactucopicrin in regulating lipid metabolism. Staining with Oil red O and measurements of triglyceride (TG) content, fatty acid β‐oxidase (FaβO) activity, reactive oxygen species (ROS) content, mitochondrial membrane potential, and adenosine triphosphate (ATP) content were used to assess the extent to which Lactucopicrin ameliorates lipid accumulation and promotes fatty acid β‐oxidation. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blot methods were used to explore the regulatory effects of Lactucopicrin on factors related to fatty acid β‐oxidation. Results showed that Lactucopicrin downregulated phosphorylated mammalian target of rapamycin (P‐mTOR) by activating the adenosine monophosphate‐activated protein kinase (AMPK) pathway and upregulated the messenger RNA (mRNA) and protein expression levels of coactivators (peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC1α)), transcription factors (peroxisome proliferator‐activated receptor α (PPARα) and peroxisome proliferator‐activated receptor γ (PPARγ)), and oxidative factors (carnitine palmitoyltransferase 1A (CPT1A) and HADHA). This phenomenon resulted in a significant increase in FaβO activity, ATP content, and JC‐1 and a significant decrease in ROS level, TG content, and intracellular lipid droplets. With the addition of Dorsomorphin, all the effects of Lactucopicrin intervention were suppressed. In summary, Lactucopicrin promotes fatty acid β‐oxidation by activating the AMPK pathway, thereby ameliorating FFA‐induced intracellular lipid accumulation in HepG2 cells.
The impact of COVID‐19 pandemic on pediatric traumatic injury and demographic presentations to a university emergency department
Authors: Arikan Mehmet , Oktay Cem 
Published: April 2024

AbstractIntroductionThe objective of this study is to investigate how lifestyle changes affect the emergency department (ED) presentations of pediatric patients with acute traumatic injuries during the COVID‐19 pandemic.MethodsThis retrospective cohort study was conducted in the ED of Akdeniz University Hospital. We analyzed the medical records of pediatric patients who presented to our ED with acute traumatic injury during the 52‐week period after March 16, 2020, which marks the official date when in‐person education was suspended in Turkey due to COVID‐19. These records were compared with those from the 52‐week period prior to March 16, 2020 (pre‐COVID). Patients with nontraumatic presentations or follow‐up visits (e.g., dressing, suture removal, injection, etc.) were excluded from the study. The data was obtained through the Hospital Information System.ResultsThe total number of ED pediatric trauma visits declined by 46%. The overall presentation rates of patients between the ages of 1 and 4 years, home injuries, falls, burns, hospitalizations, and costs per patient increased during COVID‐19. In contrast, the rate of motor vehicle accidents, sports injuries, and injuries that occurred at schools and playgrounds decreased.ConclusionsIt should be expected that the decrease in the number of acute trauma presentations due to the implementation of distance education and lockdown restrictions will increase after the pandemic. As a result, it is necessary to pay attention to the safety of children in streets, playgrounds, and schools as the pandemic ends, as much as it will continue to be important to create safe environments for children at home. In order to reduce mortality and morbidity due to pediatric traumatic injuries, all authorized people, including the relevant ministries, should take precautions to protect children from injuries, and pediatric trauma should be addressed as a preventable public health problem.
Atorvastatin‐induced intracerebral hemorrhage is inhibited by berberine in zebrafish
Authors: Liu Xin‐Yan , Chen Bo , Zhang Rui , Zhang Miao‐Qing , Ma Yuan‐Yuan , Han Ying , Jiang Jian‐Dong , Zhang Jing‐Pu 
Published: April 2024

AbstractIntracerebral hemorrhage (ICH), for which there are currently no effective preventive or treatment methods, has a very high fatality rate. Statins, such as atorvastatin (ATV), are the first‐line drugs for regulating blood lipids and treating hyperlipidemia‐related cardiovascular diseases. However, ATV‐associated ICH has been reported, although its incidence is rare. In this study, we aimed to investigate the protective action and mechanisms of berberine (BBR) against ATV‐induced brain hemorrhage. We established an ICH model in zebrafish induced by ATV (2 μM) and demonstrated the effects of BBR (10, 50, and 100 μM) on ICH via protecting the vascular network using hemocyte staining and three transgenic zebrafish. BBR was found to reduce brain inflammation and locomotion injury in ICH‐zebrafish. Mechanism research showed that ATV increased the levels of VE‐cadherin and occludin proteins but disturbed their localization at the cell membrane by abnormal phosphorylation, which decreased the number of intercellular junctions between vascular endothelial cells (VECs), disrupting the integrity of vascular walls. BBR reversed the effects of ATV by promoting autophagic degradation of phosphorylated VE‐cadherin and occludin in ATV‐induced VECs examined by co‐immunoprecipitation (co‐IP). These findings provide crucial insights into understanding the BBR mechanisms involved in the maintenance of vascular integrity and in mitigating adverse reactions to ATV.
Quantitative Assessment of Drug Efficacy and Emergence of Resistance in Patients with Metastatic Renal Cell Carcinoma Using a Longitudinal Exposure‐Tumor Growth Inhibition Model: Apitolisib (Dual PI3K/mTORC1/2 Inhibitor) Versus Everolimus (mTORC1 Inhibitor)
Authors: Moein Anita , Jin Jin Y. , Wright Matthew R. , Wong Harvey 
Published: April 2024

AbstractCancer remains a significant global health challenge, and despite remarkable advancements in therapeutic strategies, poor tolerability of drugs (causing dose reduction/interruptions) and/or the emergence of drug resistance are major obstacles to successful treatment outcomes. Metastatic renal cell carcinoma (mRCC) accounts for 2% of global cancer diagnoses and deaths. Despite the initial success of targeted therapies in mRCC, challenges remain to overcome drug resistance that limits the long‐term efficacy of these treatments. Our analysis aim was to develop a semi‐mechanistic longitudinal exposure‐tumor growth inhibition model for patients with mRCC to characterize and compare everolimus (mTORC1) and apitolisib's (dual PI3K/mTORC1/2) ability to inhibit tumor growth, and quantitate each drug's efficacy decay caused by emergence of tumor resistance over time. Model‐estimated on‐treatment tumor growth rate constant was 1.7‐fold higher for apitolisib compared to everolimus. Estimated half‐life for loss of treatment effect over time for everolimus was 16.1 weeks compared to 7.72 weeks for apitolisib, suggesting a faster rate of tumor re‐growth for apitolisib patients likely due to the emergence of resistance. Goodness‐of‐fit plots including visual predictive check indicated a good model fit and the model was able to capture individual tumor size–time profiles. Based on our knowledge, this is the first clinical report to quantitatively assess everolimus (mTORC1) and apitolisib (PI3K/mTORC1/2) efficacy decay in patients with mRCC. These results highlight the difference in overall efficacy of 2 drugs due to the quantified efficacy decay caused by emergence of resistance, and emphasize the importance of model‐informed drug development for targeted cancer therapy.
Cross‐sectional interactive associations of physical activity and sedentary behaviour with physical capacity across adulthood
Authors: Raffin Jérémy , Rolland Yves , Aubertin‐Leheudre Mylène , Aragoni da Silva Jaqueline , Guyonnet Sophie , Pillard Fabien , Vellas Bruno , de Souto Barreto Philipe ,   for the INSPIRE group
Published: April 2024

AbstractBackgroundThe way physical activity (PA) and sedentary behaviour (SB) independently and interactively modify the age‐related decline in physical capacity remains poorly understood. This cross‐sectional study investigated the independent and interactive associations of PA and SB with physical function and performance throughout the adult life course.MethodsData from 499 community‐dwelling adults (63% female) aged 20–92 years, involved in the INSPIRE Human Translational Cohort, were used in this cross‐sectional study. Daily time spent on moderate‐to‐vigorous PA (MVPA, min/day) and SB (h/day) was measured with activPAL triaxial accelerometers. Physical function and performance were assessed through the measurement of the 4‐m usual gait speed (m/s), handgrip strength (kg), lower‐limb strength (isokinetic knee extension torque, N·m), estimated lower‐limb power (five‐time chair‐rise test performance, s) and cardiorespiratory fitness (V̇O2max, mL/kg/min). Confounder‐adjusted multiple linear and curvilinear regressions were performed to investigate how MVPA, SB and their interactions were associated with the physical outcomes (all square root‐transformed except gait speed) throughout the adulthood spectrum.ResultsInteraction analyses revealed that the combination of higher levels of MVPA with lower levels of SB favourably reshaped the negative relationship between handgrip strength and age (age2 × SB × MVPA: B = −7E‐08, SE = 3E‐08, P < 0.05). In addition, higher levels of MVPA were independently associated with an improved age‐related profile in gait speed (age2 × MVPA: B = 3E‐06, SE = 1E‐06, P < 0.05), chair‐rise performance (age × MVPA: B = −9E‐05, SE = 4E‐05, P < 0.05) and V̇O2max (MVPA at 21 years: B = 3E‐02, SE = 7E‐03, P < 0.05; age × MVPA: B = −5E‐04, SE = 2E‐04, P < 0.05). Conversely, the detrimental association of age with lower‐limb muscle strength (age × SB: B = −1E‐04, SE = 6E‐05, P < 0.05) and chair‐rise performance (age × SB: B = 1E‐05, SE = 7E‐06, P < 0.05) was exacerbated with increasing duration of SB, independently of MVPA. Supplementary analyses further revealed that some of these associations were age and sex specific.ConclusionsThis cross‐sectional study demonstrated that reduced sedentary time and increased activity duration were independently and synergistically associated with an attenuated age‐related loss in physical capacity. These findings need to be confirmed with longitudinal data but encourage both adopting an active lifestyle and reducing sedentary time as preventive measures against physical aging.
Health resilience and the global pandemic: The effect of social conditions on the COVID‐19 mortality rate
Authors: Elkomy Shimaa , Jackson Tim 
Published: April 2024

AbstractCan ‘health resilience’ explain international differences in COVID‐19 mortality? This paper aims to understand the significant diversity in mortality rates between countries in terms of their degree of preparedness for the crisis and the underlying health conditions of the population. We integrate COVID‐19 data from the first year of the pandemic with panel data from 2009 to 2017 for 188 countries around the world in order to investigate international variation in COVID‐19 mortality rates. Country‐level data on health, medical, social and policy variables are compared with COVID‐19 mortality rates, with further controls imposed to adjust for infection rate, population and health spending. The results show that prior health conditions, social deprivation and the demography of the country all have significant effects on the mortality rates associated with the virus. The evidence also suggests that countries with higher levels of health‐related policy targets demonstrated lower levels of mortality during the crisis. Finally, we confirm that social habits such as smoking, alcohol consumption and over‐eating create a highly vulnerable group of individuals who were exposed to a greater risk of mortality during the outbreak.
Genetic characterization of pediatric SARI‐associated human adenoviruses in eight Chinese provinces during 2017–2021
Authors: Cai Jianlin , Liu Ying , Qian Cheng , Gao Yixuan , Zhao Sheng , Ma Yingwei , Xiang Xingyu , Xu Jing , Zhang Feng , Li Maozhong , Xu Hongmei , Li Qi , Li Chongyang , Lin Yitong , Xia Baicheng , Cui Aili , Zhang Yan , Zhu Zhen , Mao Naiying 
Published: April 2024

AbstractHuman adenovirus (HAdV) is a significant viral pathogen causing severe acute respiratory infections (SARIs) in children. To improve the understanding of type distribution and viral genetic characterization of HAdV in severe cases, this study enrolled 3404 pediatric SARI cases from eight provinces of China spanning 2017–2021, resulting in the acquisition of 112 HAdV strains. HAdV‐type identification, based on three target genes (penton base, hexon, and fiber), confirmed the diversity of HAdV types in SARI cases. Twelve types were identified, including species B (HAdV‐3, 7, 55), species C (HAdV‐1, 2, 6, 89, 108, P89H5F5, Px1/Ps3H1F1, Px1/Ps3H5F5), and E (HAdV‐4). Among these, HAdV‐3 exhibited the highest detection rate (44.6%), followed by HAdV‐7 (19.6%), HAdV‐1 (12.5%), and HAdV‐108 (9.8%). All HAdV‐3, 7, 55, 4 in this study belonged to dominant lineages circulating worldwide, and the sequences of the three genes demonstrated significant conservation and stability. Concerning HAdV‐C, excluding the novel type Px1/Ps3H1F1 found in this study, the other seven types were detected both in China and abroad, with HAdV‐1 and HAdV‐108 considered the two main types of HAdV‐C prevalent in China. Two recombinant strains, including P89H5F5 and Px1/Ps3H1F1, could cause SARI as a single pathogen, warranting close monitoring and investigation for potential public health implications. In conclusion, 5 years of SARI surveillance in China provided crucial insights into HAdV‐associated respiratory infections among hospitalized pediatric patients.
An improved small‐signal model for GaN HEMTs devices
Authors: Bai Jing , Zhang Ao , Cheng Jiali , Gao Jianjun 
Published: April 2024

AbstractAn improved small‐signal model applied to GaN‐based devices is presented in this article. The extrinsic elements of the equivalent circuit topology are extracted using the test structures and the cut‐off method. In order to obtain a good agreement between model simulations and measurements, distributed capacitive effects are taken into account. In addition, an inductance Lds is introduced based on the conventional intrinsic equivalent circuit topology. Good agreement between the modeled and measured S‐parameters is obtained for GaN HEMTs with a gate width of 2 × 100 μm (number of gate fingers × unit gate width) in the frequency range of 0.5–40 GHz.
Analyzing mastectomy and reconstruction weight in immediate autologous breast reconstruction: A preliminary study
Authors: Kim Minji , Ali Barkat , Graziano Francis D. , Colvin Kate , Boe Lillian A. , Allen Robert J. , Nelson Jonas A. , Disa Joseph 
Published: April 2024

AbstractBackgroundThis study aims to explore the ideal breast size by assessing the relationship between mastectomy to free flap weight ratio and complications as well as patient‐reported outcomes in autologous breast reconstruction (ABR).MethodA retrospective review of patients undergoing bilateral immediate ABR with mastectomy and flap weights available was completed. Patients were divided into three groups based on the ratio of mastectomy to flap weights. The patients were grouped as “maintained” if the flap weight was within 10% of the mastectomy weight. Patients with a weight difference greater than 10% were used to declare “downsized” or “upsized.” Outcomes included complications and four domains of the BREAST‐Q at 1‐year postoperatively.ResultsThree hundred and fifty‐nine patients were included in the analysis, of which 112 were downsized, 91 maintained, and 156 upsized, respectively. Presence of complications did not significantly differ among the groups. At 1‐year postoperatively, Sexual Well‐being significantly differed (p = 0.033). Between preoperative and 1 year, patients who upsized experienced an improvement in Satisfaction with Breasts by 16 points (p < 0.001), while patients who downsized experienced a decline in Physical Well‐being of the Chest by 7 points (p = 0.016). Multivariable linear regression model showed that Sexual Well‐being was 13 points lower in the downsized cohort than in the maintained cohort (β = −13, 95% confidence interval: −21 to −5.4; p = 0.001).ConclusionAlthough complication rates do not significantly differ between the three cohorts, patients who downsize may have lower Sexual Well‐being postoperatively. Surgeons should consider our preliminary findings to counsel patients preoperatively about the predicted breast size and the impact of downsizing on sexual health.
Evaluating clinical practice guidelines for the management of rectal cancer: Did they get it RIGHT?
Authors: Cai Ming , Cotter Robin R. , Wong Sandra L. , Mayo Sara W. 
Published: April 2024

AbstractBackground and ObjectivesClinical Practice Guidelines (CPGs) are crucial tools for clinicians seeking to deliver evidence‐based patient care. We utilized the Reporting Items for practice Guidelines in HealThcare (RIGHT) checklist to assess the reporting quality of CPGs addressing the management of rectal cancer.MethodsFour multidisciplinary rectal cancer CPGs published 2017–2022 were evaluated: American Society of Colon and Rectal Surgeons (ASCRS), European Society for Medical Oncology (ESMO), National Comprehensive Cancer Network (NCCN), and National Institute for Health and Care Excellence (NICE). We quantitatively assessed each CPG using the RIGHT checklist and qualitative analysis was performed to generate common themes.ResultsRIGHT checklist items fulfilled by each CPG ranged from 12 to 17 (out of 22). Each guideline demonstrated unique categories of weakness: ASCRS in Basic Information (1 of 4 items), ESMO in Evidence (1 of 3), NCCN in Recommendations (1 of 3), and NICE in Review and Quality Assurance (0 of 2). Common themes that emerged included sprase discussion on the financial aspects of rectal cancer management and lack of transparency in formulating recommendations.ConclusionsDespite their variability, each of the 22 checklist elements are present in contemporary CPGs. Utilizing the RIGHT checklist would allow experts to create guidelines adhering to high‐quality reporting standards.
On varieties whose general surface section has negative Kodaira dimension
Authors: Ciliberto Ciro , Fontanari Claudio 
Published: April 2024

AbstractIn this paper, inspired by work of Fano, Morin, and Campana–Flenner, we give a projective classification of varieties of dimension 3 whose general hyperplane sections have negative Kodaira dimension, and we partly extend such a classification to varieties of dimension whose general surface sections have negative Kodaira dimension. In particular, we prove that a variety of dimension whose general surface sections have negative Kodaira dimension is birationally equivalent to the product of a general surface section times unless (possibly) if the variety is a cubic hypersurface.
Core–Shell Double Gyroids Directed by Selective Solvation for ABC Triblock Terpolymers
Authors: Miyamori Yuta , Tong Liang , Nabae Yuta , Hatakeyama‐Sato Kan , Hayakawa Teruaki 
Published: April 2024

AbstractThe formation of ABC triblock terpolymers through solution casting is still challenging. In this study, core–shell double gyroid network structures are fabricated via solution casting using poly(2,2,2‐trifluoroethyl methacrylate) (PTFEMA) (F)‐b‐[poly(4‐vinylpyridine) (P4VP) (P)]‐b‐[polystyrene (PS) (S)] (FPS) triblock terpolymers in N,N‐dimethylformamide (DMF). Upon heat treatment, the polymer tends to form a sphere‐in‐lamellar structure at the F/S interface. Given the solubility properties of each component in DMF, it is anticipated that the effective volume fraction of F relative to P would increase in concentrated solutions and the effective volume fraction of S would decrease. The microphase‐separated structure derived from the DMF solution consistently results in the formation of a network structure composed of a core–shell double gyroid, with F as the matrix, P as the shell, and S as the core, and their periodic lengths gradually increase to 110.8, 131.8, and 162.7 nm as increase molecular weights of PS blocks to 13.8, 20.7, and 28.8 kg mol−1. Based on the solubility properties of the polymer components highlighted in this study, the solvent selection strategy is broadly applicable to ABC triblock terpolymers featuring various polymer components, offering a more efficient avenue for fabricating core–shell double gyroid structures.
Evaluation of Subjective Tinnitus Severity and Distortion Product Otoacoustic Emissions and Extended High‐Frequency Audiometry
Authors: Tekumalla Sruti , Perlov Natalie M. , Gokhale Saket , Awosanya Samiat , Urdang Zachary D. , Croce Julia , Bixler Anna , Willcox Thomas O. , Chiffer Rebecca C. , Fitzgerald Dennis 
Published: April 2024

AbstractObjectiveTinnitus is a multifactorial phenomenon with quality‐of‐life detriments for those affected by it. We aim to establish a relationship between subjective tinnitus severity with objective audiometric data in the extended high frequency (EHF) from 9 to 16 khz and with distortion product otoacoustic emissions (DPOAE). We hypothesize that severe subjective tinnitus as measured by the Tinnitus Handicap Inventory (THI) does not correlate with increased hearing thresholds in the EHF range.Study DesignProspective.SettingSingle Tertiary Care Center.MethodsPatients identified with tinnitus and normal hearing thresholds within standard frequency range (250‐8000 Hz) were consented for participation. Those with underlying otologic disease, trauma, radiotherapy, or ototoxic drug use were excluded. The THI questionnaire was given to eligible patients and audiometric test results were collected. THI scores were categorized by severity groups. An n = 20 to 30 was determined to have an effect size of 0.7 with a significance level of P = .05.ResultsTHI and audiometric data were collected for 38 patients and categorized into mild (n = 18, 47.4%), moderate (n = 8, 21.1%), slight (n = 7, 18.4%), and severe (n = 5, 13.2%) tinnitus severity groups. Mean THI score was 32.3 ± 19.6 with a statistically significant difference in scores by assigned THI severity group (P < .01). There were no significant differences or linear relationship among hearing thresholds in EHF range or DPOAE stratified by subjective tinnitus group (P = .49, r2 = 0.10)ConclusionSubjective tinnitus severity is not predictive of audiometric outcomes. This finding can be used as a counseling tool to help tinnitus patients manage symptoms, expectations, and overall treatment outcomes.