bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum matrix metalloproteinase 7 levels identifies poor prognosis advanced colorectal cancer patients
Authors: Maurel Joan , Nadal Cristina , Garcia‐Albeniz Xabier , Gallego Rosa , Carcereny Enric , Almendro Vanesa , Mármol Maribel , Gallardo Elena , Maria Augé Josep , Longarón Raquel , Martínez‐Fernandez Alex , Molina Rafael , Castells Antoni , Gascón Pere 
Published: May 2007

AbstractMetalloproteinase 7 (MMP‐7) plays an important role in tumor growth, invasion and dissemination, and is secreted to the media. Because of the close implication of MMP‐7 in cancer biology, we sought to define the prognostic significance of serum levels of MMP‐7 in metastatic colorectal cancer (CRC) and explore its possible impact in the daily clinical practice. MMP‐7 expression was determined by enzyme‐linked immunoabsorbent assay. We assessed serum MMP‐7 levels in 87 healthy controls, 96 patients with nonmetastatic CRC and 120 patients with advanced CRC. Clinical information was gathered from patient files. Cox proportional hazards model was used to assess survival. MMP‐7 and the variables associated with prognosis were entered and a backward elimination method was employed to adjust the model. Inclusion criteria was p ≤≤≤≤ 0.05 and exclusion criteria was p ≥≥≥≥ 0.10. Advanced CRC patients have a significant higher mean serum MMP‐7 levels (13.4 ng/ml) than those in nonmetastatic CRC (5.5 ng/ml; p < 0.001) and healthy controls (4.2 ng/ml; p < 0.001). In metastatic patients, after adjusting for other prognostic variables, MMP‐7 (entered as a continuous variable) is associated with decreased survival (HR 1.016, IC 95% 1.002–1.031). Serum MMP‐7 levels are significantly elevated in patients with advanced CRC. In conclusion, MMP‐7 is an independent prognostic factor for survival in advanced CRC. In our sample, the risk of death associated to MMP‐7 increase is much higher than the risk of death associated to lactate dehydrogenase elevation. © 2007 Wiley‐Liss, Inc.
Fruit and vegetable consumption and lung cancer risk: Updated information from the European Prospective Investigation into Cancer and Nutrition (EPIC)
Authors: Linseisen Jakob , Rohrmann Sabine , Miller Anthony B. , Bueno‐de‐Mesquita H. Bas , Büchner Frederike L. , Vineis Paolo , Agudo Antonio , Gram Inger T. , Janson Lars , Krogh Vittorio , Overvad Kim , Rasmuson Torgny , Schulz Mandy , Pischon Tobias , Kaaks Rudolf , Nieters Alexandra , Allen Naomi E. , Key Timothy J. , Bingham Sheila , Khaw Kay‐Tee , Amiano Pilar , Barricarte Aurelio , Martinez Carmen , Navarro Carmen , Quirós Ramón , Clavel‐Chapelon Françoise , Boutron‐Ruault Marie‐Christine , Touvier Mathilde , Peeters Petra H.M. , Berglund Göran , Hallmans Göran , Lund Eiliv , Palli Domenico , Panico Salvatore , Tumino Rosario , Tjønneland Anne , Olsen Anja , Trichopoulou Antonia , Trichopoulos Dimitrios , Autier Philippe , Boffetta Paolo , Slimani Nadia , Riboli Elio 
Published: May 2007

AbstractThe association of fruit and vegetable consumption and lung cancer incidence was evaluated using the most recent data from the European Prospective Investigation into Cancer and Nutrition (EPIC), applying a refined statistical approach (calibration) to account for measurement error potentially introduced by using food frequency questionnaire data. Between 1992 and 2000, detailed information on diet and life‐style of 478,590 individuals participating in EPIC was collected. During a median follow‐up of 6.4 years, 1,126 lung cancer cases were observed. Multivariate Cox proportional hazard models were applied for statistical evaluation. In the whole study population, fruit consumption was significantly inversely associated with lung cancer risk while no association was found for vegetable consumption. In current smokers, however, lung cancer risk significantly decreased with higher vegetable consumption; this association became more pronounced after calibration, the hazard ratio (HR) being 0.78 (95% CI 0.62–0.98) per 100 g increase in daily vegetable consumption. In comparison, the HR per 100 g fruit was 0.92 (0.85–0.99) in the entire cohort and 0.90 (0.81–0.99) in smokers. Exclusion of cases diagnosed during the first 2 years of follow‐up strengthened these associations, the HR being 0.71 (0.55–0.94) for vegetables (smokers) and 0.86 (0.78–0.95) for fruit (entire cohort). Cancer incidence decreased with higher consumption of apples and pears (entire cohort) as well as root vegetables (smokers). In addition to an overall inverse association with fruit intake, the results of this evaluation add evidence for a significant inverse association of vegetable consumption and lung cancer incidence in smokers. © 2007 Wiley‐Liss, Inc.
Folate receptor overexpression is associated with poor outcome in breast cancer
Authors: Hartmann Lynn C. , Keeney Gary L. , Lingle Wilma L. , Christianson Teresa J.H. , Varghese Bindu , Hillman David , Oberg Ann L. , Low Philip S. 
Published: May 2007

AbstractThe high affinity folate receptor is a membrane‐associated glycoprotein that is preferentially expressed in cancers of epithelial origin and rarely expressed in normal cells. We examined its expression pattern in breast cancer, utilizing a tissue microarray containing samples from 63 invasive breast cancers from women with divergent clinical outcomes. Thirty‐three women comprised the poor outcome group with a median time to recurrence of 1.9 years. Thirty women, the good outcome group, were free of recurrence for a minimum of 7 years after diagnosis. The intensity of folate receptor staining was strongly correlated with outcome. There were two summary categories of staining intensity: weak (n = 42) or strong (n = 21). In the strong staining group, 17 of 21 women (81%) have recurred and their median survival is 2.4 years. In the weak staining group, 16 of 42 women (38%) have recurred. Their median survival is not estimable. After adjustment for tumor size, nodal status, ER status, adjuvant therapy, histology and tumor grade, strong staining for the folate receptor remained significantly associated with poor outcome, p < 0.001. Our work requires validation in a larger cohort, but supports the possibility of using folate receptor‐targeted approaches in the management of breast cancer. © 2007 Wiley‐Liss, Inc.
A Comparative Study of Leaf Breakdown of Three Native Tree Species in a Slowly‐Flowing Headwater Stream in the Colombian Andes
Authors: Chara Julian , Baird Donald , Telfer Trevor , Giraldo Lina 
Published: May 2007

AbstractThe dynamics of leaf breakdown in a headwater Colombian stream were evaluated for the native tree species Myrsine guianensis, Cupania latifolia and Nectandra lineatifolia using coarse and fine mesh litter bags. Ten bags of each species (five of each mesh size) were retrieved from the stream at 1, 8, 15, 30, 60 and 120 days. k values ranged from 0.0008 to 0.0058 day–1 and density of macroinvertebrates from 35 to 55 individuals per leaf bag, peaking at day 8. Myrsine guianensis degraded more rapidly than the other species for both coarse and fine mesh bags. This species and Nectandra lineatifolia presented differences in k values between coarse and fine mesh bags, suggesting that macroinvertebrates influenced the decay rate. Despite the low densities of macroinvertebrates found, shredders represented 12.7% of individuals and 50 to 68% of the invertebrate biomass in bags, indicating that this functional feeding group was an important component of fauna associated with litter breakdown in this first order tropical stream. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Epitope mapping of the neuronal growth inhibitor Nogo‐A for the Nogo receptor and the cognate monoclonal antibody IN‐1 by means of the SPOT technique
Authors: Zander Hilke , Reineke Ulrich , Schneider‐Mergener Jens , Skerra Arne 
Published: May 2007

AbstractNogo‐A is a potent inhibitor of axonal outgrowth in the central nervous system of adult mammals, where it is expressed as a membrane protein on oligodendrocytes and in myelin. Here we describe an attempt to identify linear peptide epitopes in its sequence that are responsible for the interaction either with the Nogo receptor (NgR) or with the neutralizing monoclonal antibody IN‐1. Analysis of an array of immobilized overlapping 15 mer peptides covering the entire amino acid sequence of human Nogo‐A (1192 residues) revealed a single epitope with prominent binding activity both towards the recombinant NgR and the IN‐1 Fab fragment. Further truncation and substitution analysis yielded the minimal epitope sequence 'IKxLRRL' (x ≠ P), which occurs within the so‐called Nogo66 region (residues 1054–1120) of Nogo‐A. The bacterially produced Nogo66 fragment exhibited binding activity both for the recombinant NgR and for the IN‐1 Fab fragment on the Western blot as well as in ELISA. Unexpectedly, the synthetic epitope peptide and the recombinant Nogo66 showed cross‐reactivity with the 8‐18C5 Fab fragment, which is directed against myelin oligodendrocyte glycoprotein (MOG) as a structurally unrelated target. On the other hand, the recombinant N‐terminal domain of Nogo‐A (residues 334–966) was shown to specifically interact on the Western blot and in an ELISA with the IN‐1 Fab fragment but not with the recombinant NgR, which is in agreement with previous results. Hence, our data suggest that there is a distinct binding site for the Nogo receptor in the Nogo66 region of Nogo‐A, whereas its interaction with NgR is less specific than anticipated before. Although there probably exists a non‐linear epitope for the neutralizing antibody IN‐1 in the N‐terminal region of Nogo‐A, which is likely to be accessible from outside the cell, a previously postulated second binding site for NgR in this region (called Nogo‐A‐24) remains elusive. Copyright © 2007 John Wiley & Sons, Ltd.
Sensory and microbiological quality of shiitake mushrooms in modified‐atmosphere packages
Authors: Parentelli Carina , Ares Gastón , Corona Mauricia , Lareo Claudia , Gámbaro Adriana , Soubes Matilde , Lema Patricia 
Published: May 2007

AbstractThe aim of the present work was to study the influence of modified‐atmosphere packaging on the microbiological and sensory quality of shiitake mushrooms (Lentinula edodes). Mushrooms were packaged under atmospheric air (passive modified atmosphere) and an initial gas mixture of 5% O2 and 2.5% CO2 (active modified atmosphere), in bags of two different films: low‐density polyethylene (PE) and polypropylene (PP). As control, mushrooms were packaged in macroperforated PP films. Bags were stored at 5 °C for 20 days. Package atmosphere composition, mushroom respiration rate, weight loss, microbiological counts and sensory quality were determined during storage. Risk assays were also performed. Under the studied conditions, shiitake mushroom deterioration was not due to microorganism growth, and therefore the shelf‐life of this product might be defined by changes in its sensory characteristics. Sensory analysis showed that mushrooms stored under modified atmosphere (active and passive) had a higher deterioration rate than those stored in PP macroperforated films, and lower sensory quality values during the entire storage time. These results suggest that mushroom deterioration was probably due to shiitake mushrooms' sensitivity to high CO2 concentrations. Copyright © 2007 Society of Chemical Industry
Determination of hydroperoxides in oils and fats using kits
Authors: Osawa Cibele C , Gonçalves Lireny A G , Ragazzi Sidnei 
Published: May 2007

AbstractThe primary products of lipid oxidation are peroxides, which are usually quantified using iodometric methods, although interferent action and difficulty in determining the titration end point can lead to imprecise results. PeroxySafe™ kits determine the peroxide value (PV) on the basis of ferrous oxidation in the presence of xylenol orange in an acidic medium. They provide a fast and simple alternative requiring minimal sample sizes and generating few residues. The present study, correlating statistically the results of these kits with the official iodometric method (AOCS method Cd 8b‐90) for the determination of PVs of refined vegetable oils, lard and hidrogenated fats, proved that they can be used satisfactorily. Larger differences were found for PVs of less than 2 meq kg−1 owing to the relatively small titrant volume used in the official method. The low sensitivity of the official iodometric method was not compensated for by the use of a more dilute solution, although it was assumed that the use of a larger volume of titrant would lead to a smaller difference. The results obtained here revealed that the PVs had doubled, with the kits giving up to four times those obtained using the modification of the official method. Copyright © 2007 Society of Chemical Industry
Enzymatic synthesis of myristoyl disaccharides and their surface activity
Authors: Piao Junkui , Takase Koji , Adachi Shuji 
Published: May 2007

AbstractThe condensation of trehalose, maltose, cellobiose, sucrose, turanose, palatinose, lactose and melibiose with myristic acid by a lipase (triacylglycerol acylhydrolase; EC 3.1.1.3) from Candida antarctica was examined at 60 °C in a mixture of pyridine and 2‐methyl‐2‐propanol (20/80 by vol.). The reactivity of trehalose was the highest among the tested disaccharides, and maltose and palatinose followed. Cellobiose and lactose were poor substrates for condensation. Condensation of all the disaccharides except for cellobiose and lactose with myristic acid was carried out at various initial disaccharide concentrations to estimate the initial reaction rate. Plots of the initial rates for monomyristoyl disaccharide formation versus the initial disaccharide concentration normalized by the solubility of the disaccharide in the mixture indicated that palatinose was the best substrate, and that trehalose, turanose and maltose were the next best ones. The surface activity of the monoacyl disaccharides scarcely depended on the type of disaccharide. Copyright © 2007 Society of Chemical Industry
cDNA for phytoene desaturase in Dunaliella salina and its expressed protein as indicators of phylogenetic position of the β‐carotene biosynthetic pathway
Authors: Zhu Yue‐Hui , Jiang Jian‐Guo , Chen Xin‐Wen 
Published: May 2007

AbstractThe desaturations of phytoene are indispensable reaction phases to carotenogenesis. Full‐length cDNA for phytoene desaturase (PDS) in the chlorophyte Dunaliella salina was cloned by reverse transcriptase–polymerase chain reaction and rapid amplification of cDNA ends. The cDNA was expressed in Escherichia coli to produce an expected protein band of molecular mass 65 kDa, as well being expressed in another color complementary E. coli system in which the original phytoene desaturase coding gene crtI of the modified plasmid pACCAR16 ▵ IX was inactivated/complemented. But the colony of the E. coli system did not display the orange color which in the positive control colony appeared to be due to the accumulation of β‐carotene. It is deduced that the expressed enzyme from the green algae could only partially functionally complement the deleted bacterium CrtI, which means another carotene desaturase (ζ‐carotene desaturase) is required for formation of lycopene in D. salina, and the β‐carotene biosynthesis pathway in this alga is similar with that in cyanobacteria and higher plants. The presence of the cloned cDNA and its expressed phytoene desaturase indicated the phylogenetic position of the β‐carotene biosynthesis pathway of D. salina in the molecular evolution of carotenoid biosynthesis from bacteria to plants. Copyright © 2007 Society of Chemical Industry
Ethanol and red wine polyphenols induce the short‐term downregulation of PAI‐1 gene expression in vivo in rat aortic endothelium
Authors: Grenett Hernan E , Abou‐Agag Laila A , Olave Nelida C , Pasten Consuelo , Wolkowicz Paul E , Parks Dale A 
Published: May 2007

AbstractModerate alcohol or red wine consumption reduces the risk of cardiovascular mortality. This cardiovascular protection is likely due to the additive, combined and/or synergistic effects of alcohol itself or other components of wine, in particular polyphenols. Experiments were carried out to determine whether ethanol/polyphenols also decrease plasminogen activator inhibitor type 1 (PAI‐1) mRNA expression in vivo, using the rat as an animal model. Male Sprague–Dawley rats were gavaged with ethanol, the individual polyphenols catechin and quercetin or saline vehicle. The in vivo effect of ethanol or individual polyphenols on PAI‐1 mRNA was then assessed by in situ hybridisation and quantitative reverse transcriptase (RT) polymerase chain reaction (RT‐PCR). PAI‐1 mRNA expression was significantly reduced in the endothelial and smooth muscle cells of the thoracic aorta of all experimental rats. RT‐PCR analysis of PAI‐1 mRNA levels in vascular tissue showed a ∼55% reduction in PAI‐1 mRNA consistent with the decrease in aortic endothelium PAI‐1 mRNA observed with in situ hybridisation. This decrease may enhance endothelial cell (EC)‐mediated fibrinolytic activity in vivo. The cardioprotection afforded by moderate red wine consumption can therefore be attributed in part to the combined effects of ethanol and individual polyphenols on EC fibrinolysis. Copyright © 2007 Society of Chemical Industry
Stability of quantization dimension and quantization for homogeneous Cantor measures
Authors: Kesseböhmer Marc , Zhu Sanguo 
Published: May 2007

AbstractWe effect a stabilization formalism for dimensions of measures and discuss the stability of upper and lower quantization dimension. For instance, we show for a Borel probability measure with compact support that its stabilized upper quantization dimension coincides with its packing dimension and that the upper quantization dimension is finitely stable but not countably stable. Also, under suitable conditions explicit dimension formulae for the quantization dimension of homogeneous Cantor measures are provided. This allows us to construct examples showing that the lower quantization dimension is not even finitely stable. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Lie triple derivations on nest algebras
Authors: Lu Fangyan 
Published: May 2007

AbstractLet δ be a Lie triple derivation from a nest algebra 𝒜 into an 𝒜‐bimodule ℳ︁. We show that if ℳ︁ is a weak* closed operator algebra containing 𝒜 then there are an element S ∈ ℳ︁ and a linear functional f on 𝒜 such that δ (A) = SA – AS + f (A)I for all A ∈ 𝒜, and if ℳ︁ is the ideal of all compact operators then there is a compact operator K such that δ (A) = KA – AK for all A ∈ 𝒜. As applications, Lie derivations and Jordan derivations on nest algebras are characterized. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Hugoniot–Maslov chains of a shock wave in conservation law with polynomial flow
Authors: Rodríguez Bermúdez Panters , Valiño Alonso Baldomero 
Published: May 2007

AbstractIn this paper we give a theoretical foundation to the asymptotical development proposed by V. P. Maslov for shock type singular solutions of conservations laws, in the framework of Colombeau theory of generalized functions. Indeed, operating with Colombeau differential algebra of simplified generalized functions, we proof that Hugoniot–Maslov chains are necessary conditions for the existence of shock waves in conservation laws with polynomial flows. As a particular case, these equations include the Hugoniot–Maslov chains for shock waves in the Hopf equation. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Generators of C0‐groups with smallest spectra
Authors: Seferoglu H. 
Published: May 2007

AbstractLet T = {T (t)}t ∈ℝ be a C0‐group on a Banach space X with generator A. Under what conditions the assumption σ (A) = {0} implies that A = 0? This is called “A = 0” problem. In this paper we present some results related to this problem. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Bayesian statistical MUNE method
Authors: Henderson Robert D. , Ridall P. Gareth , Hutchinson Nicole M. , Pettitt Anthony N. , McCombe Pamela A. 
Published: May 2007

AbstractWe have developed a new method of motor unit number estimation (MUNE) for assessing diseases such as amyotrophic lateral sclerosis (ALS). We used data from the whole stimulus–response curve and then performed a Bayesian statistical analysis. The Bayesian method uses mathematical equations that express the basic elements of motor unit activation after electrical stimulation and allows for the sources of variability and uncertainty in this formulation. The Bayesian MUNE method was used to determine the most probable number of motor units in 8 normal subjects, 49 ALS subjects, and 3 subjects with progressive lower motor neuron (LMN) weakness. In normals the number of motor units was calculated to be 75–85 in hand and 40–58 in foot muscles. In ALS subjects the number of motor units per muscle was less than in normal subjects. In 17 ALS subjects and 3 subjects with LMN weakness the median, ulnar, or peroneal nerve was studied on repeated occasions over an average of 189 days (range 63–1,071) and the number of motor units progressively declined, with a half‐life ranging from 62–834 days. The results of our MUNE technique were reproducible on replicate studies. A Bayesian statistical MUNE method is a new approach that can be used to study ALS patients serially for assessment and treatment trials. Muscle Nerve, 2007
Children are more susceptible to central fatigue than adults
Authors: Streckis Vytautas , Skurvydas Albertas , Ratkevicius Aivaras 
Published: May 2007

AbstractPerformance in high‐intensity exercise is dependent on the ability to activate motor units. The main aim of this study was to test the hypothesis that adult men and women (age 19–27 years) are able to maintain higher levels of voluntary activation (VA) in knee extensor muscles than boys and girls (age 12–14 years). The volunteers (n = 7 in each group) performed three 5‐s maximal voluntary contractions (MVCs) and a continuous 2‐min MVC. The VA and fatigue of the muscles was assessed by applying 250‐ms 100‐HZ test tetani (TT100HZ). During brief MVCs girls showed lower VA than women, but the difference between boys and men was not significant. During the 2‐min MVC, VA in boys and girls was more depressed than in adults. The end‐exercise values of the relative TT100HZ torque correlated with the average VA during the exercise. Thus, the results of the study support the hypothesis that children are more susceptible to central fatigue than adults. This should be taken into account when evaluating results of fitness tests that require high levels of motor unit activation. Muscle Nerve, 2007
Muscle and nerve involvement in granulomatous mycosis fungoides
Authors: Hazrati Lili‐Naz , Bril Vera , Nag Sukriti 
Published: May 2007

AbstractOccasional cases of peripheral neuropathy have been reported in classic mycosis fungoides. A rare variant of mycosis fungoides is the granulomatous form. We describe the occurrence of myopathy and peripheral neuropathy in a young woman who had skin lesions since the age of 12 years. At the age of 20 years they were diagnosed as granulomatous mycosis fungoides. The skin lesions resolved with interferon therapy and radiation. She then presented with cardiac and pulmonary symptoms and signs that were initially thought to be due to sarcoidosis or systemic vasculitis. A nerve and muscle biopsy showed granulomatous mycosis fungoides. To our knowledge, involvement of muscle and nerve by granulomatous mycosis fungoides has not been reported previously. Early reports suggested a favorable prognosis for the granulomatous subtype of mycosis fungoides. Based on a literature review and the course in our case, however, granulomatous mycosis fungoides seems to be an indicator of aggressive disease and ultimately a poor prognosis. Muscle Nerve, 2007