bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of calcium integrin‐binding protein 1 in the mechanobiology of the liver endothelium
Authors: Wang Cong , Felli Eric , Selicean Sonia , Nulan Yeliduosi , Lozano Juan José , Guixé‐Muntet Sergi , Bosch Jaume , Berzigotti Annalisa , Gracia‐Sancho Jordi 
Published: March 2024

AbstractLiver sinusoidal endothelial cells (LSECs) dysfunction is a key process in the development of chronic liver disease (CLD). Progressive scarring increases liver stiffness in a winch‐like loop stimulating a dysfunctional liver cell phenotype. Cellular stretching is supported by biomechanically modulated molecular factors (BMMFs) that can translocate into the cytoplasm to support mechanotransduction through cytoskeleton remodeling and gene transcription. Currently, the molecular mechanisms of stiffness‐induced LSECs dysfunction remain largely unclear. Here we propose calcium‐ and integrin‐binding protein 1 (CIB1) as BMMF with crucial role in LSECs mechanobiology in CLD. CIB1 expression and translocation was characterized in healthy and cirrhotic human livers and in LSECs cultured on polyacrylamide gels with healthy and cirrhotic‐like stiffnesses. Following the modulation of CIB1 with siRNA, the transcriptome was scrutinized to understand downstream effects of CIB1 downregulation. CIB1 expression is increased in LSECs in human cirrhosis. In vitro, CIB1 emerges as an endothelial BMMF. In human umbilical vein endothelial cells and LSECs, CIB1 expression and localization are modulated by stiffness‐induced trafficking across the nuclear membrane. LSECs from cirrhotic liver tissue both in animal model and human disease exhibit an increased amount of CIB1 in cytoplasm. Knockdown of CIB1 in LSECs exposed to high stiffness improves LSECs phenotype by regulating the intracellular tension as well as the inflammatory response. Our results demonstrate that CIB1 is a key factor in sustaining cellular tension and stretching in response to high stiffness. CIB1 downregulation ameliorates LSECs dysfunction, enhancing their redifferentiation, and reducing the inflammatory response.
Insight into the molecular mechanism, cytotoxic, and anticancer activities of phyto‐reduced silver nanoparticles in MCF‐7 breast cancer cell lines
Authors: Ullah Ikram , Khalil Ali Talha , Zia Asad , Hassan Ishtiaq , Shinwari Zabta Khan 
Published: March 2024

AbstractThis contribution insight on the cytotoxic and anticancer activities and molecular mechanism of phyto‐reduced silver nanoparticles (AgNPs) in MCF‐7 breast cancer cell lines. A simple, entirely green synthesis process was optimized for the phyto‐reduction of AgNP (~12.7 nm) using aqueous leaf extracts of Indigofera heterantha. The structural and vibrational properties of biosynthesized AgNPs were extensively characterized using UV–Vis spectrophotometer, x‐ray diffraction (XRD), dynamic light scattering (DLS), and Fourier transform Infrared spectroscopy (FTIR), while their shape and morphology was studied through scanning electron microscopy (SEM). The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) cell viability assay indicates concentration dependent inhibition with IC50, 27.93 ± 2.10 μg/mL against MCF‐7 cells and 294.38 ± 3.87 μg/mL against L929 cells. The manifested anticancer mechanism in MCF‐7 cells was extensively studied using Acridine orange/ethidium bromide (AO/EB), 4′,6‐diamidino‐2‐phenylindole (DAPI) and Annexin‐V/propedium iodide fluorescence microscopic assays. The level of reactive oxygen species (ROS) was measured using DCFH‐DA fluorescent spectroscopy. Overall, the results show that AgNPs exhibit cytotoxic and apoptotic effect on breast cancer MCF‐7 cells by damaging membrane integrity and nuclear fragmentation due to oxidative stress generated by elevated level of ROS.Research Highlights
Biomimetic synthesis of nano dimension size silver nanoparticles (AgNPs).
Characterization of AgNPs through UV–Vis, DLS, XRD, FTIR, and SEM.
Cytotoxic and anticancer effects of the biosynthesized AgNPs in L929 fibroblast cells and MCF7 breast cancer respectively.
Determination of morphological, and nuclear changes triggered by AgNPs in MCF 7 breast cancer cells using fluorescent microscopy and flow cytometry.
Apoptosis induction by AgNPs in cancer cells through oxidative stress generated by reactive oxygen species (ROS).
Endemic species analysis: Foliar epidermal anatomical characters of Aster glehnii F. Schmidt (Asteraceae)
Authors: Zaman Wajid , Lee Eun Mi , Park SeonJoo 
Published: March 2024

AbstractThe classification and identification of Aster glehnii F. Schmidt are determined from its foliar epidermal anatomical features. Scanning electronic microscopy has been used to determine the foliar epidermal anatomical characteristics of the species in detail. This study compared the qualitative and quantitative characteristics of the leaf epidermis of A. glehnii for taxonomic identification to be used as a reference for future studies on the species. A. glehnii has smooth, thin cuticles, depressed anomocytic stomata dispersed randomly throughout the leaf surface, polygonal epidermal cells with straight to slightly curved anticlinal walls, and no trichomes. There are obvious veins containing thick‐walled bundle sheath cells. The stomatal density is between 100 and 150 stomata per millimeter. The vein density ranges from five to 10 veins per millimeter, and the epidermal cells are 10 to 20 μm long and 5 to 10 μm in width. Understanding the connections between the different A. glehnii species and categorizing and identifying them depend heavily on these foliar epidermal structural features. Taxonomy and conservation are closely intertwined because the former serves as the basis for comprehending and safeguarding biodiversity.Research Highlights
Optical microscopy of the A. glehnii leaf epidermis for taxonomic identification
SEM was used to identify and authenticate endemic species
Microscopic identification of endemic species can assist in the conservation
ELISA‐based detection of immunoglobulins against extracellular vesicles in blood plasma
Authors: Driedonks Tom A. P. , Jiang Linglei , Gololobova Olesia , Liao Zhaohao , Witwer Kenneth W. 
Published: March 2024

AbstractExtracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell‐derived EVs into pig‐tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV‐mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV‐based therapies in preclinical studies.
Simplified equations for wet bulb globe temperature estimation in Bangladesh
Authors: Kamal A. S. M. Maksud , Faruki Fahim Abul Kashem , Shahid Shamsuddin 
Published: March 2024

AbstractThe increasing temperatures and shifts in meteorological conditions have heightened the vulnerability of Bangladesh's densely populated regions, known for their high heat and humidity, to potential health hazards. While the Wet Bulb Globe Temperature (WBGT) is a widely acknowledged and robust measure for evaluating heat stress in various occupational and outdoor environments, its widespread application is impeded by the complexity of calculations, the substantial computational resources required, and the need for specialized expertise, particularly in developing nations. Therefore, this study aimed to develop simplified equations for estimating WBGT in Bangladesh using meteorological variables. This study applied Liljegren's model on high‐resolution reanalysis data of the European Centre for Medium‐Range Weather Forecasts (ERA5) to calculate WBGT from 1979 to 2021. Subsequently, linear and nonlinear regressions were used to derive simplified equations for estimating Liljegren WBGT in Bangladesh. The quadratic regression models offer simplified equations for WBGT estimation. The model with only temperature (Ta) as an input estimates WBGT with an R2 of 0.967 and an RMSE of 0.716, effectively capturing a significant portion of WBGT variability. The inclusion of solar radiation (SR) with Ta improved the performance, with an R2 of 0.996 and an RMSE of 0.242. The best parsimonious model, with an R2 of 0.986 and an RMSE of 0.471, is derived when wind speed is considered with Ta and SR inputs, achieving an R2 of 0.993 and an RMSE of 0.331. Comprehensive graphical and statistical analyses confirm the high accuracy of all models. The three‐input model notably demonstrates exceptional performance, attaining optimal values for critical metrics, including extreme WBGT. The results affirm the practical suitability of the derived models for accurate estimations of WBGT in Bangladesh. These equations provide simplified tools for assessing heat stress conditions, contributing to public health initiatives, occupational safety guidelines, and climate change adaptation strategies.
Humanistic principles for providing culturally sensitive corrective feedback in supervision
Authors: Holstun Vasti P. , Bohecker Lynn 
Published: March 2024

AbstractCorrective feedback can be a difficult endeavor in supervision. This article focuses on the following six principles of humanistic education that can facilitate corrective feedback: a nonthreatening environment, affective bias, self‐evaluation, connectedness, self‐determination, and personal growth orientation. These principles offer a framework for providing culturally sensitive supervision and corrective feedback. Implications for counseling supervision are included.
Electronic and vibrational properties of MgCu2O3
Authors: Chandra Somesh , Kaur Gurpreet , S. Abhaya , Shukla Balmukund , V. Srihari , Bhalerao Gopalkrishna M. , R. Govindaraj 
Published: March 2024

AbstractThe vibrational properties of orthorhombic MgCu2O3 compound have been investigated within the framework of the density functional perturbation theory (DFPT) as well as experimentally, to validate the computational results. MgCu2O3 was synthesized by solid‐state reaction and characterized by synchrotron‐based X‐ray diffraction, Raman spectroscopy, and Fourier transform infrared spectroscopy. The DFT + U‐based first principle calculations were performed to obtain the correct electronic ground state and the band structure of this compound. The same DFT + U methodology was employed along with DFPT calculations for obtaining vibrational properties: phonon density of states and phonon band structure. The atomic vibrations for each mode were also analyzed, and the Raman and the IR active modes are identified. Experimentally observed Raman and infrared (IR) spectra agree well with the computed ones.
Multidisciplinary Advanced Surgical Planning for Slide Tracheoplasty Using 3D‐Printed Models
Authors: Richardson Clare M. , Walton Scott , Park Jason S. , Bonilla‐Velez Juliana , Bly Randall A. , Dahl John P. , Parikh Sanjay R. , Friedman Seth , Johnson Kaalan E. 
Published: March 2024

ObjectiveThe objective of this study was to develop and assess multidisciplinary advanced surgical planning (ASP) sessions using three dimensional (3D) printed models for cervicothoracic slide tracheoplasty (CST). We hypothesized that these sessions would improve surgeon confidence, streamline intraoperative planning, and highlight the utility of 3D modeling.Methods3D‐printed patient‐specific trachea models were used in pre‐operative ASP sessions consisting of a multidisciplinary case discussion and hands‐on slide tracheoplasty simulation. Participants completed a survey rating realism, utility, impact on the final surgical plan, and pre‐ and post‐session confidence. Statistical analysis was performed via Wilcoxon and Kruskal‐Wallis tests.ResultsForty‐eight surveys were collected across nine sessions and 27 different physicians. On a 5‐point Likert scale, models were rated as “very realistic”, “very useful” (both median of 4, IQR 3–4 and 4–5, respectively). Overall confidence increased by 1.4 points (+/− 0.7, p < 0.0001), with the largest change seen in those with minimal prior slide tracheoplasty experience (p = 0.005). Participants felt that the sessions “strongly” impacted their surgical plan or anticipated performance (median 4, IQR 4–5), regardless of training level or experience.Conclusion3D‐printed patient‐specific models were successfully implemented in ASP sessions for CST. Models were deemed very realistic and very useful by surgeons across multiple specialties and training levels. Surgical planning sessions also strongly impacted the final surgical plan and increased surgeon confidence for CST.Level of Evidence4 Laryngoscope, 134:3395–3401, 2024
Similarity Network Analysis of the Adaptive Immune Response in the Proximal Airway
Authors: Clark Evan , Talatala Edward Ryan R , Ye Wenda , Davis Ruth J , Collins Samuel L , Hillel Alexander T , Ramirez‐Solano Marisol , Sheng Quanhu , Wanjalla Celestine N , Mallal Simon A , Gelbard Alexander 
Published: March 2024

ObjectivesRecent immunologic study of the adaptive immune repertoire in the subglottic airway demonstrated high‐frequency T cell clones that do not overlap between individuals. However, the anatomic distribution and antigenic target of the T cell repertoire in the proximal airway mucosa remain unresolved.MethodsSingle‐cell RNA sequencing of matched scar and unaffected mucosa from idiopathic subglottic stenosis patients (iSGS, n = 32) was performed and compared with airway mucosa from healthy controls (n = 10). T cell receptor (TCR) sequences were interrogated via similarity network analysis to explore antigenic targets using the published algorithm: Grouping of Lymphocyte Interactions by Paratope Hotspots (GLIPH2).ResultsThe mucosal T cell repertoire in healthy control airways consisted of highly expressed T cell clones conserved across anatomic subsites (trachea, bronchi, bronchioles, and lung). In iSGS, high‐frequency clones were equally represented in both scar and adjacent non‐scar tissue. Significant differences in repertoire structure between iSGS scar and unaffected mucosa was observed, driven by unique low‐frequency clones. GLIPH2 results suggest low‐frequency clones share targets between multiple iSGS patients.ConclusionHealthy airway mucosa has a highly conserved T cell repertoire across multiple anatomic subsites. Similarly, iSGS patients have highly expressed T cell clones present in both scar and unaffected mucosa. iSGS airway scar possesses an abundance of less highly expanded clones with predicted antigen targets shared between patients. Interrogation of these shared motifs suggests abundant adaptive immunity to viral targets in iSGS airway scar. These results provide insight into disease pathogenesis and illuminate new treatment strategies in iSGS.Level of EvidenceNA Laryngoscope, 134:3245–3252, 2024
Ansa Cervicalis External Branch Anastomosis to Restore High Tone Voice in Thyroid Surgery
Authors: Miyauchi Akira , Fujishima Makoto , Masuoka Hiroo , Sasaki Takahiro , Miya Akihiro , Ishisaka Tomo , Adachi Shiori , Kawano Shiori , Matsunaga Akihide , Yamamoto Masashi , Higashiyama Takuya , Ito Yasuhiro , Onoda Naoyoshi , Kihara Minoru 
Published: March 2024

ObjectivesInjury to the external branch of the superior laryngeal nerve (EBSLN) causes low‐pitch voice and voice fatigue, particularly in female subjects, and available treatments are limited. Here, we assess a novel surgical procedure to restore a high‐tone voice: ansa cervicalis to EBSLN anastomosis (A‐E anastomosis).MethodsBetween November 2012 and April 2022, 13 patients (12 female) underwent unilateral EBSLN resection and A‐E anastomosis, while 20 (16 female) underwent EBSLN resection during thyroid surgery. Patients (4494 women and 1025 men) with normal laryngoscopy scheduled for thyroid surgery served as normal controls. Phonatory function was examined using a Phonation Analyzer PA‐1000 preoperatively and intermittently postoperatively.ResultsIn patients who underwent A‐E anastomosis, high‐tone voice pitch decreased significantly postoperatively (673.9–471.5 Hz, p = 0.047), with restoration achieved within 5 months. The mean voice pitch in female patients who underwent A‐E anastomosis, EBSLN resection, and controls were 580.4, 522.8, and 682.0 Hz, respectively, indicating a significant decrease in EBSLN resection patients than controls (p = 0.002). The (mean – 1SD) of high‐tone voice pitch in female controls was 497 Hz; exceeding this may indicate recovery to a high‐tone voice. Overall, 73% (8/11) of A‐E anastomosis patients exceeded this value, which was marginally larger than the 43% (6/14) who underwent EBSLN resection. Data on male subjects are limited. There were no cases of adverse functional or cosmetic events.ConclusionsA‐E anastomosis, a novel simple procedure, restored high‐tone voice to some extent without any adverse events and thus warrants further investigation.Level of Evidence3 Laryngoscope, 134:3868–3873, 2024
ABCG2 polymorphisms and susceptibility to ARV‐associated hepatotoxicity
Authors: Singh HariOm , Dhotre Kishore , Shyamveer  , Choudhari Ranjana , Verma Amita , Mahajan Supriya D. , Ali Nemat 
Published: March 2024

AbstractBackgroundThe ABCG2 421C/A polymorphism contributes significantly to the distribution and absorption of antiretroviral (ARV) regimens and is associated with the undesirable side effects of efavirenz.MethodsTo investigate this, we examined ABCG2 34G/A (rs2231137) and 421C/A (rs2231142) genetic variations in 149 HIV‐infected patients (116 without hepatotoxicity, 33 with ARV‐induced hepatotoxicity) and 151 healthy controls through the PCR‐restriction fragment length polymorphism (PCR‐RFLP) technique.Results and DiscussionThe ABCG2 34GA genotype and 34A allele indicated a risk for antiretroviral therapy‐associated hepatotoxicity development (p = 0.09, OR = 1.58, 95% CI: 0.93–2.69; p = 0.06, OR = 1.50, 95% CI: 0.98–2.30). The haplotype GA was associated with hepatotoxicity (p = 0.042, OR = 2.37, 95% CI: 1.04–5.43; p = 0.042, OR = 2.49, 95% CI: 1.04–5.96). Moreover, when comparing HIV patients with hepatotoxicity to healthy controls, the haplotype GA had an association with an elevated risk for the development of hepatotoxicity (p = 0.041, OR = 1.73, 95% CI: 1.02–2.93). Additionally, the association of the ABCG2 34GA genotype with the progression of HIV (p = 0.02, OR = 1.97, 95% CI: 1.07–3.63) indicated a risk for advanced HIV infection. Furthermore, the ABCG2 421AA genotype was linked to tobacco users and featured as a risk factor for the progression of HIV disease (p = 0.03, OR = 11.07, 95% CI: 1.09–270.89).ConclusionThe haplotype GA may enhance the risk of hepatotoxicity development and its severity. Individuals with the ABCG2 34A allele may also be at risk for the development of hepatotoxicity. Additionally, individuals with an advanced stage of HIV and the ABCG2 34GA genotype may be at risk for disease progression.
Dynamics of extracellular vesicle uptake by mural granulosa cells in mice
Authors: Edure Taichi , Matsuno Yuta , Matsushita Kodai , Maruyama Natsumi , Fujii Wataru , Naito Kunihiko , Sugiura Koji 
Published: March 2024

AbstractExtracellular vesicles (EVs) play an important role in the development and function of mammalian ovarian follicles. However, the mechanisms by which they are taken up by the follicular granulosa cells remain unclear. In addition, while oocytes play a pivotal role in follicular development, the possible interactions between oocyte‐derived paracrine factors (ODPFs) and EV signals are unknown. Therefore, this study aimed to elucidate the mechanism of EV uptake and the effects of ODPFs on EV uptake by follicular somatic mural granulosa cells in mice. Fluorescence‐labeled transferrin (TRF) and cholera toxin B (CTB), substrates for clathrin‐ and caveolae‐mediated endocytosis, respectively, were taken up by mural granulosa cells in vitro. Their uptake was inhibited by Pitstop 2 and genistein, inhibitors of clathrin and caveolae pathways, respectively. Mural granulosa cells took up EVs, and this uptake was suppressed by Pitstop 2 and genistein. Moreover, ODPFs promoted the uptake of EVs and CTB, but not TRF, by mural granulosa cells. These results suggest that mural granulosa cells take up EVs through both clathrin‐ and caveolae‐mediated endocytosis and that oocytes may promote caveolae‐mediated endocytosis to facilitate the uptake of EVs.
Efficient stochastic modal decomposition methods for structural stochastic static and dynamic analyses
Authors: Zheng Zhibao , Beer Michael , Nackenhorst Udo 
Published: March 2024

AbstractThis article presents unified and efficient stochastic modal decomposition methods to solve stochastic structural static and dynamic problems. We extend the idea of deterministic modal decomposition method for structural dynamic analysis to stochastic cases. Standard/generalized stochastic eigenvalue equations are adopted to calculate the stochastic subspaces for stochastic static/dynamic problems and they are solved by an efficient reduced‐order method. The stochastic solutions of both static and dynamic equations are approximated by stochastic bases of the stochastic subspaces. Original stochastic static/dynamic equations are then transformed into a set of single‐degree‐of‐freedom (SDoF) stochastic static/dynamic equations, which are efficiently solved by the proposed non‐intrusive methods. Specifically, a non‐intrusive stochastic Newmark method is developed for the solution of SDoF stochastic dynamic equations, and the element‐wise division of sample vectors is used to solve the SDoF stochastic static equations. All of these methods have low computational effort and are weakly sensitive to the stochastic dimension, thus the proposed methods avoid the curse of dimensionality successfully. Two numerical examples, including two‐ and three‐dimensional spatial problems with low and high stochastic dimensions, are given to show the efficiency and accuracy of the proposed methods.
Pattern formation in dense populations studied by inference of nonlinear diffusion‐reaction mechanisms
Authors: Srivastava Siddhartha , Garikipati Krishna 
Published: March 2024

SummaryReaction‐diffusion systems have been proposed as a model for pattern formation and morphogenesis. The Fickian diffusion typically employed in these constructions model the Brownian motion of particles. The biological and chemical elements that form the basis of this process, like cells and proteins, occupy finite mass and volume and interact during migration. We propose a Reaction‐Diffusion system with Maxwell‐Stefan formulation to construct the diffusive flux. This formulation relies on inter‐species force balance and provides a more realistic model for interacting elements. We also present a variational system inference‐based technique to extract these models from spatiotemporal data for these processes. We show that the inferred models can capture the characteristics of local Turing instability that instigates the pattern formation process. Moreover, the equilibrium solutions of the inferred models form similar patterns to the observed data.