bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of Major Tendon Ruptures and Anterior Cruciate Ligament Tears in US Army Soldiers
Authors: White Daniel W. , Wenke Joseph C. , Mosely Dan S. , Mountcastle Sally B. , Basamania Carl J. 
Published: April 2007

Background Although a rare event, the prevalence of major tendon rupture has increased in recent decades. Identification of risk factors is important for prevention purposes. Hypothesis Race is a risk factor for major tendon ruptures. Study Design Cohort study (prevalence); Level of evidence, 2. Methods All patients admitted for surgical management of a rupture of a major tendon at Womack Army Medical Center, Fort Bragg, North Carolina, in 1995 and 1996 were identified and evaluated for risk factors. Results The authors identified 52 major tendon ruptures 29 Achilles, 12 patellar, 7 pectoralis major, and 4 quadriceps tendon ruptures. All patients were active-duty soldiers, and 1 was a female soldier. Forty-one tendon ruptures occurred among black soldiers, 8 occurred among white soldiers, and 3 occurred among Latino soldiers. The population at risk included 93 224 exposures during the 2-year period, of which 67.1% were white, 24.5% were black, and 8.4% were self-classified as other race. The rate ratio for tendon rupture, adjusted for gender and age, was 13.3 (95% confidence interval, 6.2-28.5) between blacks and whites and 2.9 (95% confidence interval, 0.8-10.9) between Latinos and whites. Conclusion The rate of major tendon rupture was 13 times greater for black men in this study population when compared with whites. Interventions among those at a higher risk for injury should be considered.
Deficits in Neuromuscular Control of the Trunk Predict Knee Injury Risk
Authors: Zazulak Bohdanna T. , Hewett Timothy E. , Reeves N. Peter , Goldberg Barry , Cholewicki Jacek 
Published: April 2007

Background Female athletes are at significantly greater risk of anterior cruciate ligament (ACL) injury than male athletes in the same high-risk sports. Decreased trunk (core) neuromuscular control may compromise dynamic knee stability. Hypotheses (1) Increased trunk displacement after sudden force release would be associated with increased knee injury risk; (2) coronal (lateral), not sagittal, plane displacement would be the strongest predictor of knee ligament injury; (3) logistic regression of factors related to core stability would accurately predict knee, ligament, and ACL injury risk; and (4) the predictive value of these models would differ between genders. Study Design Cohort study (prognosis); Level of evidence, 2. Methods In this study, 277 collegiate athletes (140 female and 137 male) were prospectively tested for trunk displacement after a sudden force release. Analysis of variance and multivariate logistic regression identified predictors of risk in athletes who sustained knee injury. Results Twenty-five athletes (11 female and 14 male) sustained knee injuries over a 3-year period. Trunk displacement was greater in athletes with knee, ligament, and ACL injuries than in uninjured athletes (P < .05). Lateral displacement was the strongest predictor of ligament injury (P = .009). A logistic regression model, consisting of trunk displacements, proprioception, and history of low back pain, predicted knee ligament injury with 91% sensitivity and 68% specificity (P = .001). This model predicted knee, ligament, and ACL injury risk in female athletes with 84%, 89%, and 91% accuracy, but only history of low back pain was a significant predictor of knee ligament injury risk in male athletes. Conclusions Factors related to core stability predicted risk of athletic knee, ligament, and ACL injuries with high sensitivity and moderate specificity in female, but not male, athletes.
The Routine Culture of Allograft Tissue in Anterior Cruciate Ligament Reconstruction
Authors: Guelich David R. , Lowe Walter R. , Wilson Bubba 
Published: April 2007

Background Allograft tissue is an acceptable alternative to autograft tissue in anterior cruciate ligament (ACL) reconstruction. However, several infections associated with tissue procurement have led some to consider routine intraoperative cultures of allograft tissue before implantation. A positive culture result presents a treatment dilemma in the asymptomatic patient. Hypothesis Treatment of culture swab—positive allograft tissue is unnecessary if there is no evidence of clinical infection. Study Design Cohort study (prognosis); Level of evidence, 2. Methods Retrospective analysis was performed on 247 cultures taken in 321 consecutive ACL reconstructions from a single surgeon. Allograft cultures were taken intraoperatively before antibiotic washing and implantation. All patients received standard prophylactic antibiotics consisting of intravenous vancomycin and perioperative cefazolin, and were routinely monitored in the postoperative period for signs of infection. Results Twenty-four of 247 (9.7%) cultures were positive after implantation. Sixteen of these (67%) grew organisms of high pathogenicity, whereas 8 (33%) were of low pathogenicity. Cultures were classified as poor, scant, or rare in all 24 patients. These patients did not receive additional antibiotics, and none went on to develop septic arthritis or wound complications. The 2 cases of septic arthritis had negative intraoperative cultures. Conclusions Treatment of low-virulence organisms or minimal growth high-virulence organisms is unnecessary if no evidence of clinical infection exists. The results may call into question the utility of routinely culturing allograft tissue as positive results did not correlate with infectious complications.
Emotions and feelings in the Bible: analysis of the Pentateuch's affective lexicon
Authors: Galati Dario , Miceli Renato , Tamietto Marco 
Published: May 2007

English This study aims to investigate how affective states are described in the Old Testament. Three psychology researchers were asked to read the first five books of the first Italian version (from the 18th century) of the Old Testament (Pentateuch, or Torah) and to select all the terms that referred to an emotion or a feeling. For each selected term, they also had to pinpoint its position in the text (i.e. book, chapter) and the various characteristics of the affective episode in which it appeared (i.e. experiencing subject, situational antecedent, intentional object, instrumental behaviors). The textual analysis showed that the affective terms most frequently cited referred to four categories: “fear, awe”, “anger, hate”, “affliction, pain, sadness” and “love, joy, happiness”. These categories were significantly associated with specific instrumental behaviors and characters of the narration. Multivariate analysis also indicated that the frequency of citation of the affective categories varied significantly as a function of the book in which they appeared. In the conclusions, the authors discuss the conception of emotions and feelings issuing from the Pentateuch analysis. French L'article se propose d'analyser de quelle manière les expériences affectives sont décrites et évaluées dans la Bible. On a demandé à trois chercheurs psychologues de lire les cinq premiers livres de la première version italienne (du 18ème siècle) de la Bible (le Pentateuque, ou Torah) et d'y sélectionner tous les termes se référant à des expériences affectives (émotions et sentiments). Pour chaque terme sélectionné, ils devaient aussi indiquer sa position dans le texte (livre, chapitre) et les différentes composantes de l'épisode au cours duquel l'émotion ou le sentiment étaient expérimentés (le sujet qui expérimentait l'expérience affective, la situation qui la causait, l'objet intentionnel auquel elle se référait, le comportement réactif du sujet, l'évaluation morale de l'expérience). Les résultats de la sélection ont mis en évidence que les termes affectifs les plus fréquents se réfèrent à quatre catégories d'émotions, à savoir, dans l'ordre, la peur, la colère, la tristesse, la joie. Ces émotions sont expérimentées à des fréquences différentes par les principaux personnages de la narration (Dieu, les hommes, le Peuple d'Israël). L'émotion le plus souvent expérimentée par Dieu est la colère et par les différents personnages humains, la peur. Aucune évaluation morale négative quant aux expériences affectives n'émerge de l'analyse du texte. L'analyse multivariée met en évidence le fait que la fréquence des citations des catégories émotionnelles change sensiblement en fonction des cinq livres du Pentateuque. Dans leurs conclusions, les auteurs examinent la conception implicite des émotions et des sentiments qui semble émerger du Pentateuque.
Allogeneic cytotoxic T-cell therapy for EBV-positive posttransplantation lymphoproliferative disease: results of a phase 2 multicenter clinical trial
Authors: Haque Tanzina , Wilkie Gwen M. , Jones Marie M. , Higgins Craig D. , Urquhart Gillian , Wingate Phoebe , Burns David , McAulay Karen , Turner Marc , Bellamy Christopher , Amlot Peter L. , Kelly Deirdre , MacGilchrist Alastair , Gandhi Maher K. , Swerdlow Anthony J. , Crawford Dorothy H. 
Published: April 2007

AbstractWe present the results of a multicenter clinical trial using Epstein-Barr virus (EBV)–specific cytotoxic T lymphocytes (CTLs) generated from EBV-seropositive blood donors to treat patients with EBV-positive posttransplantation lymphoproliferative disease (PTLD) on the basis of the best HLA match and specific in vitro cytotoxicity. Thirty-three PTLD patients who had failed on conventional therapy were enrolled. No adverse effects of CTL infusions were observed and the response rate (complete or partial) in 33 patients was 64% at 5 weeks and 52% at 6 months. Fourteen patients achieved a complete remission, 3 showed a partial response, and 16 had no response at 6 months (5 died before completing treatment). At 5 weeks, there was a significant trend toward better responses with higher numbers of CD4+ cells in infused CTL lines (P = .001) that were maintained at 6 months (P = .001). Patients receiving CTLs with closer HLA matching responded better at 6 months (P = .048). Female patients responded better than male patients, but the differences were not statistically significant. Our results show that allogeneic CTLs are a safe and rapid therapy for PTLD, bypassing the need to grow CTLs for individual patients. The response rate in this poor prognosis patient group is encouraging.
Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita
Authors: Alter Blanche P. , Baerlocher Gabriela M. , Savage Sharon A. , Chanock Stephen J. , Weksler Babette B. , Willner Judith P. , Peters June A. , Giri Neelam , Lansdorp Peter M. 
Published: April 2007

Abstract
Dyskeratosis congenita (DC) is an inherited bone marrow failure syndrome in which the known susceptibility genes (DKC1, TERC, and TERT) belong to the telomere maintenance pathway; patients with DC have very short telomeres. We used multicolor flow fluorescence in situ hybridization analysis of median telomere length in total blood leukocytes, granulocytes, lymphocytes, and several lymphocyte subsets to confirm the diagnosis of DC, distinguish patients with DC from unaffected family members, identify clinically silent DC carriers, and discriminate between patients with DC and those with other bone marrow failure disorders. We defined “very short” telomeres as below the first percentile measured among 400 healthy control subjects over the entire age range. Diagnostic sensitivity and specificity of very short telomeres for DC were more than 90% for total lymphocytes, CD45RA+/CD20− naive T cells, and CD20+ B cells. Granulocyte and total leukocyte assays were not specific; CD45RA− memory T cells and CD57+ NK/NKT were not sensitive. We observed very short telomeres in a clinically normal family member who subsequently developed DC. We propose adding leukocyte subset flow fluorescence in situ hybridization telomere length measurement to the evaluation of patients and families suspected to have DC, because the correct diagnosis will substantially affect patient management.
The ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum stabilizes spectrin tetramers and suppresses further invasion
Authors: Pei Xinhong , Guo Xinhua , Coppel Ross , Bhattacharjee Souvik , Haldar Kasturi , Gratzer Walter , Mohandas Narla , An Xiuli 
Published: April 2007

AbstractThe malaria parasite Plasmodium falciparum releases the ring-infected erythrocyte surface antigen (RESA) inside the red cell on entry. The protein migrates to the host cell membrane, where it binds to spectrin, but neither the nature of the interaction nor its functional consequences have previously been defined. Here, we identify the binding motifs involved in the interaction and describe a possible function. We have found that spectrin binds to a 108–amino acid fragment (residues 663-770) of RESA, and that this RESA fragment binds to repeat 16 of the β-chain, close to the labile dimer-dimer self-association site. We further show that the RESA fragment stabilizes the spectrin tetramer against dissociation into its constituent dimers, both in situ and in solution. This is accompanied by enhanced resistance of the cell to both mechanical and thermal degradation. Resealed erythrocytes containing RESA663-770 display resistance to invasion by merozoites of P falciparum. We infer that the evolutionary advantage of RESA to the parasite lies in its ability to prevent invasion of cells that are already host to a developing parasite, as well as possibly to guard the cell against thermal damage at the elevated body temperatures prevailing in febrile crises.
Toll-like receptor 2 signaling is a mediator of apoptosis in herpes simplex virus-infected microglia
Authors: Aravalli Rajagopal N , Hu Shuxian , Lokensgard James R 
Published: May 2007

AbstractBackgroundInformation regarding the response of brain cells to infection with herpes simplex virus (HSV)-1 is needed for a complete understanding of viral neuropathogenesis. We have recently demonstrated that microglial cells respond to HSV infection by producing a number of proinflammatory cytokines and chemokines through a mechanism involving Toll-like receptor 2 (TLR2). Following this cytokine burst, microglial cells rapidly undergo cell death by apoptosis. We hypothesized that TLR2 signaling might mediate the cell death process as well.MethodsTo test this hypothesis, we investigated HSV-induced cell death of microglia obtained from both wild-type and TLR2-/-mice. Cell death was studied by oligonucleosomal ELISA and TUNEL staining, and the mechanisms of apoptosis were further analyzed using murine apoptosis-specific microarrays. The data obtained from microarray analysis were then validated using quantitative real-time PCR assays.ResultsHSV infection induced apoptotic cell death in microglial cells from wild-type as well as TLR2 cells. However, the cell death at 24 h p.i. was markedly lower in knockout cells. Furthermore, microarray analyses clearly showed that the expression of pro-apoptotic genes was down-regulated at the time when wild-type cells were actively undergoing apoptosis, indicating a differential response to HSV in cells with or without TLR2.ConclusionWe demonstrate here that HSV induces an apoptotic response in microglial cells which is mediated through TLR2 signaling.
LPS induces rapid IL-10 release by M-CSF-conditioned tolerogenic dendritic cell precursors
Authors: Kwan Wing-Hong , Boix Charlotte , Gougelet Nicolas , Fridman Wolf H , Mueller Chris G F 
Published: May 2007

AbstractDendritic cells (DC) obtained by culturing myeloid precursors in GM-CSF undergo maturation and induce an efficient T cell response when stimulated with microbial products. DC precursors themselves also recognize microbial products, and it remains unclear how these stimulated DC precursors modulate the immune response. We show here that M-CSF-conditioned human DC precursors responded to LPS, Mycobacteria bovis, and inflammatory cytokines by a rapid and robust production of IL-10, largely superior to that observed with immature DC or monocytes. The endogenous IL-10 restrained the DC precursors from converting into professional APC, as blocking the IL-10 receptor in the presence of LPS resulted in the formation of efficient T cell stimulators. LPS stimulation concomitant with DC differentiation gave rise to immature DC, which were tolerant to a secondary LPS exposure. Furthermore, the LPS-activated DC precursors reduced bystander DC maturation and anti-CD3/CD28-triggered T cell activation. These data suggest that when exposed to inflammatory or microbial signals, M-CSF-conditioned DC precursors can participate in the modulation of inflammation and immune response by rapid release of IL-10.
The inflammasome in pathogen recognition and inflammation
Authors: Sutterwala Fayyaz S , Ogura Yasunori , Flavell Richard A 
Published: May 2007

AbstractThe nucleotide-binding oligomerization domain-like receptor (NLR) family of proteins is involved in the regulation of innate immune responses and cell death pathways. Some NLR family members promote the activation of proinflammatory caspases within multiprotein complexes, called inflammasomes. Recent studies analyzing mice deficient in various components of the inflammasome have provided insight into the role of these molecules in host defense against pathogens and in autoinflammatory disorders. Here, we review these studies and propose that membrane disruption leads to activation of the inflammasome.
Emergency Department Operational Changes in Response to Pay‐for‐performance and Antibiotic Timing in Pneumonia
Authors: Pines Jesse M. , Hollander Judd E. , Lee Hoi , Everett Worth W. , Uscher‐Pines Lori , Metlay Joshua P. 
Published: May 2007

Background:The percentage of adult patients admitted with pneumonia who receive antibiotics within four hours of hospital arrival is publicly reported as a quality and pay‐for‐performance measure by the Department of Health and Human Services and is called PN‐5b. Objectives:To determine attitudes among physician leaders at emergency medicine training programs toward using PN‐5b as a quality measure for pay for performance, and to determine what operational changes academic emergency departments (EDs) have made to ensure early antibiotic administration for patients with pneumonia. Methods:The authors administered an online questionnaire to 129 chairpersons and medical directors of 135 academic ED training programs in the United States on attitudes toward performance measurement in pneumonia and changes that academic EDs have made in response to PN‐5b; one response was sought from each institution. Respondents were identified through the Society for Academic Emergency Medicine Web site and e‐mailed five times to maximize survey participation. Results:Ninety chairpersons and medical directors (70%) completed the survey; 47% were medical directors, 51% were chairpersons, and 2% were medical directors and chairpersons. Forty‐five (50%) did not agree that PN‐5b was an accurate quality measure, and 61 (69%) did not agree that pay for performance targeting this measure would lead to improved pneumonia care. The most common strategy to address PN‐5b was to provide information to providers on the importance of early treatment with antibiotics (n = 63; 70%). For patients with suspected pneumonia, 46 (51%) automate chest radiograph (CXR) ordering at triage, 37 (41%) prioritize patients with suspected pneumonia, and 33 (37%) administer antibiotics before obtaining CXR results. Overall ED changes include improved turnaround time for CXR (n= 33; 37%), prioritized CXRs over other radiographs (n= 13; 14%), and improved inpatient bed availability (n= 12; 13%). Of 13 strategies identified to improve PN‐5b, the median number that programs have implemented is five (interquartile range, 5–7). All sites reported engaging in at least three operational changes to address PN‐5b. Conclusions:All EDs in this study have addressed early antibiotic administration with multiple operational changes despite mixed sentiment that these changes will improve care. Future research is needed to measure the impact of pay‐for‐performance initiatives.
Prognostic Significance of Immune Subset Measurement in Individuals With AIDS-Associated Kaposi's Sarcoma
Authors: Stebbing Justin , Sanitt Adam , Teague Alastair , Powles Tom , Nelson Mark , Gazzard Brian , Bower Mark 
Published: May 2007

Purpose A prognostic index for AIDS-associated Kaposi's sarcoma (KS) diagnosed in the era of highly active antiretroviral therapy (HAART) was based on routine clinical and laboratory characteristics. Because immune subset measurement is often performed in HIV-positive individuals, we examined whether these were predictive of mortality independently of the prognostic index, or could predict time to progression of KS. Patients and Methods We performed univariate and multivariate Cox regression analyses on a data set of 326 individuals with AIDS-associated KS to identify immune subset covariates predictive of overall survival and time to progression. Adaptive (CD8 T cell and CD19 B cell) and innate (CD16/56 natural-killer cell) immune parameters were studied by flow cytometry. Results In univariate analyses, all three immune subsets had significant effects on overall survival (P < .025). In multivariate analyses including the prognostic index, only CD8 counts remained significant (P = .026), although its effect on the overall prognostic index is small. An increase of 100 cells/mm3 in the CD8 count confers a 5% improvement in overall survival. Individuals with a higher CD8 count did not have an increased time to progression. Patients who were already on HAART at the time of KS diagnosis did not have a shorter time to progression than those who were antiretroviral naïve at KS diagnosis. Conclusion The CD8 count appears to provide independent prognostic information in individuals with AIDS-associated KS. Measurement of the CD8 count is clinically useful in patients with KS.