bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel approach for detecting global epigenetic alterations associated with tumor cell aneuploidy
Authors: Habano Wataru , Sugai Tamotsu , Jiao Yu‐Fei , Nakamura Shin‐Ichi 
Published: June 2007

AbstractAlthough aneuploidy is commonly observed in human cancers, the molecular mechanism underlying aneuploidization remains unclear. We used multiploid cancer model that had diploid and aneuploid cancer cells within the same cancerous tissue and attempted to detect specific epigenetic alterations associated with tumor cell aneuploidy. Thirty‐four multiploid colorectal cancers were subjected to crypt isolation and cell sorting, and paired diploid and aneuploid cancer cells were separated from each cancerous tissue. A methylated CpG island amplification provided a considerable number of CpG sequences that showed different methylation status between the above 2 cell populations. BLAST homology search revealed 24 different candidates (11 hypermethylated and 13 hypomethylated) from these sequences. The putative promoter sequence of the SALL4 (sal‐like 4, a human homolog to Drosophila spalt) gene was particularly more frequently hypermethylated in aneuploid cells (62%) than diploid ones (35%) in the 34 multiploid cancers. Moreover, such hypermethylation occurred more often in aneuploid cancers (8 of 16, 50%) than diploid cancers (3 of 18, 17%). In combination with demethylation study on cultured cells, these results implied a possible association between epigenetic silencing of SALL4 and tumor cell aneuploidy. SALL4 may be one of important key players that act as “caretakers” for chromosomal stability. Our new approach is a powerful tool for the global identification of such key players. © 2007 Wiley‐Liss, Inc.
Survival of squamous cell carcinoma of the head and neck in relation to human papillomavirus infection: Review and meta‐analysis
Authors: Ragin Camille C. R. , Taioli Emanuela 
Published: June 2007

AbstractHuman papillomavirus (HPV) has been associated with head and neck squamous cell carcinomas (HNSCC), especially of the oropharynx, with highest distribution in the tonsils. HPV infection has been associated with improved outcome, although not all the studies show consistent results. The reason for this is not clear. We reviewed all published articles and conducted a meta‐analysis on the overall relationship between HPV infection and overall survival (OS) and disease‐free survival (DFS) in HNSCC. Patients with HPV‐positive HNSCC had a lower risk of dying (meta HR: 0.85, 95% CI: 0.7–1.0), and a lower risk of recurrence (meta HR: 0.62, 95%CI: 0.5–0.8) than HPV‐negative HNSCC patients. Site‐specific analyses show that patients with HPV‐positive oropharyngeal tumours had a 28% reduced risk of death (meta HR: 0.72, 95%CI: 0.5–1.0) in comparison to patients with HPV‐negative oropharyngeal tumours. Similar observations were made for DFS (meta HR: 0.51, 95% CI: 0.4–0.7). There was no difference in OS between HPV‐positive and negative non‐oropharyngeal patients. The observed improved OS and DFS for HPV‐positive HNSCC patients is specific to the oropharynx; these tumours may have a distinct etiology from those tumours in non‐oropharyngeal sites. © 2007 Wiley‐Liss, Inc.
Gene therapy using ets‐1 transcription factor decoy for peritoneal dissemination of gastric cancer
Authors: Taniguchi Hirokazu , Fujiwara Yoshiyuki , Doki Yuichiro , Sugita Yurika , Sohma Itsuro , Miyata Hiroshi , Takiguchi Shuji , Yasuda Takushi , Tomita Naruya , Morishita Ryuichi , Monden Morito 
Published: June 2007

AbstractThe ets‐1 transcription factor plays an important role in cell proliferation, differentiation, apoptosis and tissue remodeling. Aberrant ets‐1 expression correlates with aggressive tumor behavior and poorer prognosis in patients with various malignancies. This study evaluated the efficacy of double‐stranded decoy oligonucleotides targeting ets‐1‐binding cis elements for the suppression of ets‐1 in treatment of a peritoneal dissemination model of gastric cancer. In vitro, MTT assay was performed to evaluate the effect of the ets‐1 decoy on cell growth. Electrophoretic mobility shift assay (EMSA) was performed to determine ets‐1 activity. In vivo, the effect of the ets‐1 decoy was investigated in the peritoneal dissemination nude mice model. Disseminated nodules were analyzed immunohistochemically. Ets‐1 decoy, but not scrambled decoy, significantly inhibited cell growth in 2 gastric cancer cell lines, which showed overexpression of ets‐1 protein by inhibiting the binding activity of ets‐1. In the peritoneal dissemination model, the ets‐1 decoy significantly suppressed the disseminated nodules, and tended to prolong the survival rate. PCNA index, microvessel density and VEGF expression were also reduced in peritoneal tumors treated with ets‐1 decoy. Intraperitoneal injection of ets‐1 decoy inhibited peritoneal dissemination of gastric cancer in a nude mice model. The results indicate that the decoy strategy for ets‐1 offers a promising therapy for patients with incurable peritoneal dissemination of gastric cancer, most of which show overexpression of ets‐1 protein. © 2007 Wiley‐Liss, Inc.
An irrigation management model for a multi‐cropping and multi‐pattern setting
Authors: Ricketts Dwight D. , Rudra Ramesh P. , Gharabaghi Bahram 
Published: June 2007

AbstractThis paper presents a user‐friendly tool for water budgeting and forecasting for multi‐plot fields to optimise the area under production. This tool will help the farmer in managing available water, ensuring that all the crops can be irrigated daily to meet current evapotranspiration demands. The “micro‐management” model is universal in application and facilitates the generation of additional information with alteration of the requisite parameter values and/or algorithms. The model is suited for increased degrees of management and operational challenges that are faced by irrigation systems serving fields, which are comprised of multi‐tenureship, and cropping patterns. Illustration of the model is carried out on small undulating landholdings in Beacon, Jamaica where multi‐cropping is being proposed with limited water supply. Copyright © 2007 John Wiley & Sons, Ltd.
Multiple myeloma: A prototypic disease model for the characterization and therapeutic targeting of interactions between tumor cells and their local microenvironment
Authors: Mitsiades Constantine S. , Mitsiades Nicholas S. , Richardson Paul G. , Munshi Nikhil C. , Anderson Kenneth C. 
Published: June 2007

AbstractThe interaction between tumor cells and the local milieu where are homing has recently become the focus of extensive research in a broad range of malignancies. Among them, multiple myeloma (MM) is now recognized as a prototypical tumor model for the characterization of these interactions. This is due not only to the propensity of MM cells to target the skeleton and form lytic bone lesions, but because interactions of MM cells with normal cells of the bone milieu can attenuate the anti‐tumor activity of conventional therapies, such as glucocorticoids and standard cytotoxic agents, including alkylators. Herein, we highlight the recent advances in our understanding of cellular and molecular mechanisms of interactions between MM cells and their milieu. Particular emphasis is placed on the interface between MM cells and normal cell compartments of the BM, especially bone marrow stromal cells (BMSCs), and on the development of a series of new classes of therapeutic agents, including the proteasome inhibitor bortezomib, thalidomide and lenalidomide, which counteract specific aspects of those MM–BM interactions. The significant clinical activity of these novel therapies has not only led to a new era in the therapeutic management of this disease, but also underscored the importance of comprehensively characterizing the role of the local microenvironment in the pathophysiology of human neoplasias. J. Cell. Biochem. 101: 950–968, 2007. © 2007 Wiley‐Liss, Inc.
Mechanotransduction from the ECM to the genome: Are the pieces now in place?
Authors: Gieni Randall S. , Hendzel Michael J 
Published: June 2007

AbstractA multitude of biochemical signaling processes have been characterized that affect gene expression and cellular activity. However, living cells often need to integrate biochemical signals with mechanical information from their microenvironment as they respond. In fact, the signals received by shape alone can dictate cell fate. This mechanotrasduction of information is powerful, eliciting proliferation, differentiation, or apoptosis in a manner dependent upon the extent of physical deformation. The cells internal “prestressed” structure and its “hardwired” interaction with the extra‐cellular matrix (ECM) appear to confer this ability to filter biochemical signals and decide between divergent cell functions influenced by the nature of signals from the mechanical environment. In some instances mechanical signaling through the tissue microenvironment has been shown to be dominant over genomic defects, imparting a normal phenotype on cells that otherwise have transforming genetic lesions. This mechanical control of phenotype is postulated to have a central role in embryogenesis, tissue physiology as well as the pathology of a wide variety of diseases, including cancer. We will briefly review studies showing physical continuity between the external cellular microenvironment and the interior of the cell nucleus. Newly characterized structures, termed nuclear envelope lamina spanning complexes (NELSC), and their interactions will be described as part of a model for mechanical transduction of extracellular cues from the ECM to the genome. J. Cell. Biochem. 104: 1964–1987, 2008. © 2007 Wiley‐Liss, Inc.
Synergistic induction of osteopontin by aldosterone and inflammatory cytokines in mesangial cells
Authors: Gauer Stefan , Hauser Ingeborg A. , Obermüller Nicholas , Holzmann Yvonne , Geiger Helmut , Goppelt‐Struebe Margarete 
Published: June 2007

AbstractHypertensive nephrosclerosis is characterized by activation of the renin–angiotensin–aldosterone system in combination with an inflammatory response characterized by an infiltration of T‐cells and mononuclear cells, which release proinflammatory cytokines like IL‐1β/TNFα. In various models of experimental hypertensive disease the chemokine osteopontin (OPN) enhances further leukocyte infiltration. Therefore, we investigated the induction of OPN expression in renal mesangial cells (MCs) by aldosterone and the inflammatory cytokines IL‐1β/TNFα. Incubation with aldosterone resulted in a time‐ and concentration‐dependent increase in OPN mRNA and protein. OPN mRNA expression followed a biphasic time course with an early increase between 4 and 8 h and the second phase starting at 14 h. The early phase was independent of protein synthesis, indicating a direct effect of aldosterone. Aldosterone‐mediated induction of OPN was prevented by spironolactone, indicative of a receptor‐mediated aldosterone effect. The mineralocorticoid receptor (MR) was identified in MCs by RT‐PCR and immunoprecipitation, and shown to interact with a putative aldosterone‐response element of the OPN promoter. The proinflammatory cytokines IL‐1β and TNFα only marginally affected OPN expression in MCs. However, coincubation of aldosterone and the cytokines synergistically increased OPN mRNA and protein levels. Since the synergistic effect on OPN mRNA was inhibited by diphenyleneiodonium, we assume an involvement of reactive oxygen species (ROS). We conclude that the chemokine OPN is a target gene of aldosterone in renal MCs, which is activated via the MR, and that proinflammatory cytokines enhance aldosterone‐dependent OPN expression. In vivo, this may result in further leukocyte infiltration aggravating hypertensive nephrosclerosis. J. Cell. Biochem. 103: 615–623, 2008. © 2007 Wiley‐Liss, Inc.
Interleukin‐4 antagonizes oncostatin M and transforming growth factor beta‐induced responses in articular chondrocytes
Authors: El Mabrouk Mohammed , Qureshi Hamid Yaqoob , Li Wen Qing , Sylvester Judith , Zafarullah Muhammad 
Published: June 2007

AbstractOncostatin M (OSM) stimulates cartilage degradation in rheumatoid arthritis (RA) by inducing matrix metalloproteinases (MMPs) and aggrecanases (ADAMTS; a disintegrin and metalloproteinase with thrombospondin motif). Transforming growth factor beta (TGF‐β1) induces cartilage repair in joints but in excessive amounts, promotes inflammation. OSM and TGF‐β1 also induce tissue inhibitor of metalloproteinase‐3 (TIMP‐3), an important natural inhibitor of MMPs, aggrecanases, and tumor necrosis factor alpha converting enzyme (TACE), the principal proteases involved in arthritic inflammation and cartilage degradation. We studied cartilage protective mechanisms of the antiinflammatory cytokine, interleukin‐4 (IL‐4). IL‐4 strongly (MMP‐13 and TIMP‐3) or minimally (ADAMTS‐4) suppressed OSM‐induced gene expression in chondrocytes. IL‐4 did not affect OSM‐stimulated phosphorylation of extracellular signal‐regulated kinases (ERKs), protein 38 (p38), c‐Jun N‐terminal kinase (JNK) and Stat1. Lack of additional suppression with their inhibitors suggested that MMP‐13, ADAMTS‐4, and TIMP‐3 inhibition was independent of these mediators. IL‐4 also downregulated TGF‐β1‐induced TIMP‐3 gene expression, Smad2, and JNK phosphorylation. Additional suppression of TIMP‐3 RNA by JNK inhibitor suggests JNK implication. The cartilage protective effects of IL‐4 in animal models of arthritis may be due to its inhibition of MMPs and ADAMTS‐4 expression. However, suppression of TIMP‐3 suggests caution for using IL‐4 as a cartilage protective therapy. J. Cell. Biochem. 103: 588–597, 2008. © 2007 Wiley‐Liss, Inc.
The stem cell hypothesis in head and neck cancer
Authors: Graziano A. , d'Aquino R. , Tirino V. , Desiderio V. , Rossi A. , Pirozzi G. 
Published: June 2007

AbstractCancer stem cells (CSCs) are tumoral cells which have stem features such as self‐renewal, high migration capacity, drug resistance, high proliferation abilities. In the last 10 years the pathological meaning and the existence of CSCs have been matter of discussion and a large number of articles have been published about the role that these cells play in the development and maintenance of the tumors. Head and neck squamous‐cell carcinoma (HNSCC) is the sixth most common cancer worldwide: early diagnosis of high‐risk premalignant lesions are high priorities for reducing deaths due to head and neck cancer. In the last years the CSCs hypothesis has been faced also for head and neck cancer, with the aim of a better comprehension of the tumor biology and an early diagnosis. The evidence that the development of a tumor comes from a small number of cells with stem‐like characteristic, could bring too to the identification of therapies against these cellular target, fundamental for maintenance and progression of the lesion. Here, a literature review has been reported about the detection of supposed CSCs in head and neck cancer. J. Cell. Biochem. 103: 408–412, 2008. © 2007 Wiley‐Liss, Inc.
Novel hormone “receptors”
Authors: Nemere Ilka , Hintze Korry 
Published: June 2007

AbstractOur concepts of hormone receptors have, until recently, been narrowly defined. In the last few years, an increasing number of reports identify novel proteins, such as enzymes, acting as receptors. In this review we cover the novel receptors for the hormones atrial naturetic hormone, enterostatin, hepcidin, thyroid hormones, estradiol, progesterone, and the vitamin D metabolites 1,25(OH)2D3 and 24,25(OH)2D3. J. Cell. Biochem. 103: 401–407, 2008. © 2007 Wiley‐Liss, Inc.
New all‐atom force field for molecular dynamics simulation of an AlPO4‐34 molecular sieve
Authors: Praprotnik Matej , Hočevar Stanko , Hodošček Milan , Penca Matej , Janežič Dušanka 
Published: June 2007

AbstractA force field of the triclinic framework of AlPO4‐34, important in methanol–hydrocarbon conversion reactions, was developed using an empirical potential function. Molecular dynamics simulation of an AlPO4‐34 triclinic framework segment of 1216 atoms, containing the template molecules isopropylamine and water, was performed with explicit consideration of atomic charges. The average RMS difference between instantaneous positions of the framework atoms during 1 ns simulation and their positions in the structure determined from single crystal X‐ray diffraction was calculated, and the average structure of the flexible framework was determined. The computed Debye‐Waller factors and simulated FTIR spectra are in good agreement with the experimental data. The new force field permits detailed molecular dynamics simulations of flexible, charged aluminophosphate molecular sieves which should lead to a better understanding of the catalytic processes and the crucial role played by templating molecules. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008
Every finite solvable group with a unique element of order two, except the quaternion group, has a symmetric sequencing
Authors: Anderson B. A. , Ihrig E. C. 
Published: June 2007

AbstractAll finite solvable groups that have symmetric sequencings are characterized. LetGbe a finite solvable group. It is shown thatGhas a symmetric sequencing if and only ifGhas a unique element of order two and is not the quaternion group. All finite groups with a unique element of order two such that the order of the group is not divisible by three are solvable and thus, except for the quaternion group, have symmetric sequencings. A crucial step used in the proof of these facts is a construction showing that if a finite groupHhas a normal subgroupCof odd order such thatH/Cadmits a 2‐sequencing, thenHadmits a 2‐sequencing. The results of this article can be viewed as generalizing a theorem of Gordon about Abelian groups and as extending the idea of a starter, suitably modified, to a large class of groups of even order by showing the existence of the required object. © 1993 John Wiley & Sons, Inc.
The use of hill‐climbing to construct orthogonal steiner triple systems
Authors: Gibbons Peter B. , Mathon Rudolf 
Published: June 2007

AbstractIn a related article, Colbourn, Gibbons, Mathon, Mullin, and Rosa [7] have shown that a pair of orthogonal Steiner triple systems exists for all v ≡ 1, 3 (mod 6), v ≥ 7 and v ≠ 9. This result is based on the construction of a finite set of pairs of orthogonal Steiner triple systems followed by the application of recursive constructions to settle the remaining undecided cases. In this article we report on the computational aspects of that investigation, and in particular the remarkable success of the hill‐climbing method. © 1993 John Wiley & Sons, Inc.
A symmetric 2‐(160, 54, 18) design
Authors: Spence Edward , Tonchev Vladimir D. , van Trung Tran 
Published: June 2007

AbstractWe give a construction for a symmetric 2‐(160, 54, 18) design that effectively uses the incidence matrix of the design of the hyperplanes in PG(3, 3) as a tactical decomposition. This is the first design ever found with these parameters. © 1993 John Wiley & Sons, Inc.
Embedding directed and undirected partial cycle systems of index λ > 1
Authors: Lindner C. C. , Rodger C. A. 
Published: June 2007

AbstractRecent results have found small embeddings for partial m‐cycle systems of order n with λ= 1. However, if λ> 1 then the best known techniques produce embeddings that are often quadratic functions of both m and n and linear functions of λ. In this article we obtain embeddings for partial m‐cycle systems of order n, and of partial directed m‐cycle systems, for all values of m. These embeddings are independent of λ and linear in both n and m. © 1993 John Wiley & Sons, Inc.
The t‐designs with prescribed automorphism group, new simple 6‐designs
Authors: Schmalz Bernd 
Published: June 2007

AbstractWe introduce an algorithm for the construction of a complete system of representatives of t‐designs with given parameters t − (v, k, λ) and prescribed full automorphism group A. It is based on the following observation published by Kramer and Mesner in 1976: The t − (v, k, λ) designs admitting automorphism group A are exactly the 0‐1‐solutions \documentclass{article}\pagestyle{empty}\begin{document}$ \overrightarrow x $\end{document} of the following system of linear equations

M are incidence matrices, which we compute by means of double cosets. Representing the set of all solutions of the above system of equations implicitly by a graph gives us the possibility either to extract the solutions explicitly or to compute their precise numbers, which often are very big. We use the lattice of overgroups of A in the full symmetric group Sv for the construction or enumeration of the isomorphism types of the t‐designs with full automorphism group A from these solutions. To the best of our knowledge our approach for the first time allows one to compute the precise number of isomorphism types or even these designs themselves for substantial numbers. We determined the (number of) isomorphism types for many known parameter sets and found new simple 6‐designs with parameters

and full automorphism group PΓL2(27). We constructed all isomorphism types of these designs; their precise numbers are 3,367,21 743,38 277, respectively. © 1993 John Wiley & Sons, Inc.
Maximal difference matrices of order q
Authors: Pott Alexander 
Published: June 2007

AbstractRecently, A. B. Evans proved the following Theorem: There is a maximal set of (p − 3)/2 [(resp. (p − 1)/2] mutually orthogonal Latin squares of order p if p is a prime p ≡ 3 mod 4 (resp. p ≡ 1 mod 4). In this article I will give a slightly different proof using more geometric arguments and results of Rédei. Further, I discuss possible generalizations of Evans' Theorem to the case of Latin squares whose order is a prime power. © 1993 John Wiley & Sons, Inc.
Carnosine interaction with nitric oxide and astroglial cell protection
Authors: Nicoletti Vincenzo Giuseppe , Santoro Anna Maria , Grasso Giulia , Vagliasindi Laura Irene , Giuffrida Maria Laura , Cuppari Christian , Purrello Vittoria Spina , Stella Anna Maria Giuffrida , Rizzarelli Enrico 
Published: June 2007

AbstractThe neuropeptide carnosine (β‐amyloid peptide aggregation has been demonstrated. Carnosine protection against peroxynitrite damage is particularly relevant, but until now there has been no evidence of any direct interaction with nitric oxide. In this study we examined the protection that carnosine provides against nitric oxide (NO)–induced cell death in primary rat astroglial cell cultures treated with lipopolysaccharide (LPS) and interferon gamma (INFγ), a well‐known neurotoxic proinflammatory condition. A correlation was found between cell protection and NO free‐radical scavenging activity of carnosine. Moreover, by competitive spectrophotometric measurement and electrospray mass spectrometry analysis in cell‐free experiments, we demonstrated a direct interaction of the dipeptide with NO. A comparison of carnosine with its homologues or derivatives (homocarnosine and carcinine) as well as with its amino acid constituents (L‐histidine and β‐alanine) highlighted that only histidine showed significant scavenging activity. Therefore, carnosine shows direct NO‐trapping ability and may be a valuable multifunctional molecule in the treatment of neurodegenerative disorders. © 2007 Wiley‐Liss, Inc.