bluuebunny/crossref_metadata_2025_split huggingface dataset:
EBV lymphoproliferative disease of host origin after haploidentical stem cell transplantation
Authors: Kasow Kimberly A. , Leung Wing , Horwitz Edwin M. , Woodard Paul , Handgretinger Rupert , Hale Gregory A. 
Published: January 2006

AbstractPost‐transplant lymphoproliferative disease (PTLPD), due to the reactivation of Epstein–Barr virus (EBV), is a serious complication. The risk of the disorder increases with T‐cell depletion methods, mismatched hematopoietic stem cell transplantation (HSCT), graft‐versus‐host disease (GVHD), and immunosuppression. In contrast to solid organ transplantation, where EBV is typically of recipient origin, the source of the EBV in HSCT recipients is donor‐derived B‐lymphocytes. In this report, we describe a 15‐year‐old girl who underwent HSCT from her father as treatment for acute myeloid leukemia (AML). She subsequently developed disseminated PTLPD involving multiple organ and nodal sites. Her neoplastic lymphoblasts were host‐derived and refractory to rituximab treatment due to lack of CD20 expression. Pediatr Blood Cancer 2007;49:869–872. © 2006 Wiley‐Liss, Inc.
An analysis of primary site control and late effects according to local control modality in non‐metastatic Ewing sarcoma
Authors: Paulino Arnold C. , Nguyen Thanh X. , Mai Wei Y. 
Published: January 2006

AbstractPurposeTo examine prognostic factors for primary site control and analyze late effects according to local treatment modality in non‐metastatic Ewing sarcoma (ES).Materials and MethodsFrom 1976 to 2001, 76 patients with localized ES and a median age of 14.5 years were seen and treated at one institution. Tumors were located in the extremity in 38, pelvis in 13, spine in 11, trunk in 8, and head and neck in 6. Tumor size was ≤8 cm in 44 and >8 cm in 32. Local therapy included radiotherapy (RT) alone in 40, surgery (S) alone in 27, and surgery followed by postoperative radiotherapy (S + RT) in 9. Chemotherapy (CT) was delivered to 65 patients (86%). Median follow‐up for surviving patients was 9.7 years.ResultsThe 5‐ and 10‐year overall survival rates were 57.5% and 52.1% while the 5‐ and 10‐year local control rate was 79.2%. The 5‐ and 10‐year local control rates were 78.2% for RT, 77.6% for surgery, and 88.9% for S + RT (P = 0.68). Multivariate analysis showed that only the use of CT was found to be a prognostic factor for local control (P = 0.014). The 5‐ and 10‐year local control rates were 83.7% for those receiving CT and 51.1% for those not receiving CT. For patients followed at least 5 years from diagnosis, late effects were seen in 10 of 19 (52.6%) receiving RT, 2 of 5 (40%) receiving S + RT, and 4 of 16 (25%) receiving surgery alone. The most common late effects with RT were muscular atrophy, limb length growth delay, and development of second malignancy. Scoliosis and decrease range of motion of an extremity were seen regardless of local treatment modality. Three patients who had surgery alone had an amputation whereas two who had RT had an amputation secondary to relapse or development of osteosarcoma.ConclusionIn this single institution study, the use of CT was the only factor found to impact on local control. Late effects were common regardless of local control strategy. Pediatric Blood Cancer © 2006 Wiley‐Liss, Inc.
Low prevalence of complications in severe neutropenic children with cancer in the unprotected environment of an overnight camp
Authors: Tabori Uri , Jones Heather , Malkin David 
Published: January 2006

AbstractBackgroundThe high risk of infection and other complications in severely neutropenic pediatric oncology patients receiving chemotherapy has led to development of a variety of preventive measures including isolation and diet restrictions. In order to examine the potential impact of these measures, we evaluated the outcomes of such patients attending a recreational summer camp.MethodsWe collected data on all children who attended an overnight summer camp for children with cancer during the years 1999–2004, and who were either severely neutropenic or at a high‐intensity phase of chemotherapy. Outcome measures included fever, bleeding, hospitalization, and clinical or laboratory evidence of infection. The observation period included both, the 2‐week camp experience and 30 days post‐camp.ResultsThe study group was comprised of 34 patients. Although nine (24%) were hospitalized for management of fever and neutropenia, only one patient had clinical or culture‐positive evidence of an invasive infectious agent. No bleeding episode was recorded and most patients attended all camp activities.ConclusionsOur results support the safety and feasibility of severely neutropenic patients with cancer to attend the non‐isolated, non‐sterile environment of a summer camp. These findings may be applicable to school and other social settings. Pediatr Blood Cancer 2007;48:148–151. © 2006 Wiley‐Liss, Inc.
Quantitative bone mineral density assessment in malignant infantile osteopetrosis
Authors: Kaste Sue C. , Kasow Kimberly A. , Horwitz Edwin M. 
Published: January 2006

AbstractPurposeTo investigate the use of quantitative computed tomography (QCT) and dual energy absorptiometry (DXA) for assessing bone mineral density (BMD) in the evaluation of children with malignant infantile osteopetrosis (MIOP).Methods and PatientsWe retrospectively reviewed QCT‐ and DXA‐determined BMD in six patients with infantile osteopetrosis and correlated BMD measured during the initial evaluation with patient characteristics.ResultsFive male and one female infant met the eligibility criteria. BMD was markedly elevated in all patients as determined by each modality, QCT or DXA. For QCT, in which age‐specific normal values are known, the BMD was found to be 22.4–32.6 standard deviations above the mean. Using DXA, the estimated BMD of the lumbar spine ranged from 0.45 to 0.8 g/cm2; children with similarly appearing bone radiographs had quite disparate BMD. While the qualitative trend of BMD among the patients was similar for both measures, the units and numerical values of BMD differed. We found no correlation between BMD results and hematopoiesis observed in the bone marrow or with visual evaluation of the radiographs.ConclusionBoth QCT and DXA are effective quantitative measures of BMD in children with MIOP. However, the same modality should be employed for longitudinal evaluation of a given patient. Each technique has unique advantages and disadvantages and may complement one another in the evaluation of MIOP or other bone disorders. Quantitative assessment of BMD in children with MIOP will likely serve to further characterize this disease. Pediatr Blood Cancer 2007;48:181–185. © 2006 Wiley‐Liss, Inc.
Effects of human monoclonal antibody 216 on B‐progenitor acute lymphoblastic leukemia in vitro
Authors: Bieber Marcia M , Twist Clare J , Bhat Neelima M , Teng Nelson N H 
Published: January 2006

AbstractBackgroundHuman monoclonal antibody (mAb) 216 is a naturally occurring IgM cytotoxic mAb that binds to a glycosylated epitope on the surface of B‐lymphocytes. This study investigated if this mAb could bind and kill acute lymphoblastic leukemia (ALL) B‐progenitor lymphoblasts in vitro. ALL cell lines were used to determine if combining mAb 216 with chemotherapeutic drugs would enhance killing and cell lines were used to measure cytotoxicity by mAb 216 with human complement.ProcedureExpression of cell surface markers and mAb 216 epitope on fresh and banked ALL bone marrow samples was determined by flow cytometry. Fresh lymphoblasts were incubated for 20 hr with mAb 216 without complement to measure cytotoxicity. Cytotoxicity of ALL cell lines incubated with mAb 216 and vincristine (VCR) or human complement was determined using flow cytometry.ResultsPre‐B‐ALL cells but not T‐ALL cells are bound and killed by mAb 216. The combination of mAb 216 and VCR at sub‐therapeutic levels demonstrated enhanced cytotoxicity beyond that observed for either agent alone. Incubation of mAb 216 with human complement increased cytotoxicity of ALL cell lines.ConclusionThis increased cytotoxicity with chemotherapy and the functional ability of mAb 216 to use multiple pathways to induce cell death identify mAb 216 as a potentially novel therapeutic tool in the treatment of B‐progenitor ALL. Based on the results from this preclinical study, a Phase I clinical trial with mAb 216 for the treatment of patients with relapsed or refractory B‐lineage ALL is ongoing. Pediatric Blood Cancer © 2006 Wiley‐Liss, Inc.
Electronic Fock space as associative superalgebra
Authors: Panin A. I. 
Published: January 2006

AbstractA new algebraic structure on a finite dimensional electronic Fock space is studied in detail. This structure is defined in terms of a certain multiplication of many electron wave functions and has close interrelation with coupled cluster and similar approaches. Its study simplifies the mathematical backgrounds of these approaches. Even more, it leads to relations that would be very difficult to derive using conventional technique. Formulas for action of the creation‐annihilation operators on products of state vectors are derived. Explicit expressions for action of simplest particle‐conserving products of the creation‐annihilation operators on powers of state vectors are given. © 2006 Wiley Periodicals, Inc. Int J Quantum Chem, 2006
Use of simulation and modelling to develop a sustainable production system
Authors: Taplin David M. R. , Spedding Trevor A. , Khoo Hsien H. 
Published: January 2006

AbstractThis paper seeks to establish a practical definition of the term sustainable development, and relate it to a company's performance. Simulation and modelling tools are applied to gain a holistic view of the activities associated with a metal casting company, which includes production, transportation and recycling. In the simulation model, four case scenarios are investigated, including the comparison of original and re‐designed cast products of 20% runner weight reduction, and the recycling of scrap metal at two different remelters. The focus of the case study is to monitor the amount of scrap, dross, carbon dioxide emissions from both energy consumption and transportation, productivity and costs during the metal material cycle. From the case study, various ‘sustainability indicators’ are highlighted. Copyright © 2006 John Wiley & Sons, Ltd and ERP Environment.
Young adult outcome of attention deficit hyperactivity disorder: a controlled 10-year follow-up study
Authors: BIEDERMAN JOSEPH , MONUTEAUX MICHAEL C. , MICK ERIC , SPENCER THOMAS , WILENS TIMOTHY E. , SILVA JULIE M. , SNYDER LINDSEY E. , FARAONE STEPHEN V. 
Published: January 2006

Background. Our objective was to estimate the lifetime prevalence of psychopathology in a sample of youth with and without attention deficit hyperactivity disorder (ADHD) through young adulthood using contemporaneous diagnostic and analytic techniques.Method. We conducted a case-control, 10-year prospective study of ADHD youth. At baseline, we assessed consecutively referred male, Caucasian children with (n=140) and without (n=120) DSM-III-R ADHD, aged 6–18 years, ascertained from psychiatric and pediatric sources to allow for generalizability of results. At the 10-year follow-up, 112 (80%) and 105 (88%) of the ADHD and control children, respectively, were reassessed (mean age 22 years). We created the following categories of psychiatric disorders: Major Psychopathology (mood disorders and psychosis), Anxiety Disorders, Antisocial Disorders (conduct, oppositional-defiant, and antisocial personality disorder), Developmental Disorders (elimination, language, and tics disorder), and Substance Dependence Disorders (alcohol, drug, and nicotine dependence), as measured by blinded structured diagnostic interview.Results. The lifetime prevalence for all categories of psychopathology were significantly greater in ADHD young adults compared to controls, with hazard ratios and 95% confidence intervals of 6·1 (3·5–10·7), 2·2 (1·5–3·2), 5·9 (3·9–8·8), 2·5 (1·7–3·6), and 2·0 (1·3–3·0), respectively, for the categories described above.Conclusions. By their young adult years, ADHD youth were at high risk for a wide range of adverse psychiatric outcomes including markedly elevated rates of antisocial, addictive, mood and anxiety disorders. These prospective findings provide further evidence for the high morbidity associated with ADHD across the life-cycle and stress the importance of early recognition of this disorder for prevention and intervention strategies.
Inference of gene regulatory networks and compound mode of action from time course gene expression profiles
Authors: Bansal Mukesh , Gatta Giusy Della , di Bernardo Diego 
Published: January 2006

Abstract
Motivation: Time series expression experiments are an increasingly popular method for studying a wide range of biological systems. Here we developed an algorithm that can infer the local network of gene–gene interactions surrounding a gene of interest. This is achieved by a perturbation of the gene of interest and subsequently measuring the gene expression profiles at multiple time points. We applied this algorithm to computer simulated data and to experimental data on a nine gene network in Escherichia coli.
Results: In this paper we show that it is possible to recover the gene regulatory network from a time series data of gene expression following a perturbation to the cell. We show this both on simulated data and on a nine gene subnetwork part of the DNA-damage response pathway (SOS pathway) in the bacteria E. coli.
Contact: dibernardo@tigem.it
Supplementary information: Supplementary data are available at
Twice‐A‐Day Quadruple Therapy for Eradication of Helicobacter pylori in the Elderly
Authors: Dore Maria Pina , Maragkoudakis Emmanouil , Pironti Antonella , Tadeu Vincenza , Tedde Rinaldo , Realdi Giuseppe , Delitala Giuseppe 
Published: January 2006

AbstractBackground:  Midday and evening twice‐a‐day quadruple therapy appears to be the most effective therapy for Helicobacter pylori infection in Northern Sardinia, a site where antibiotics resistance is common.Aim:  The objective of our study was to estimate the efficacy, side‐effects, and compliance of a quadruple therapy containing esomeprazole in a group of dyspeptic elderly patients.Patients and Methods:  Consecutive elderly patients positive for H. pylori infection and not previously treated for eradication were enrolled. Therapy consisted of esomeprazole 20 mg, tetracycline 500 mg, metronidazole 500 mg, and bismuth subcitrate tablets 240 mg, all twice‐a‐day with the midday and evening meals, for 10 days. Efficacy was evaluated using 13C‐urea breath testing. Compliance was assessed after completing treatment and at follow up. Side effects were graded based on daily activities.Results:  Ninety‐five dyspeptic patients (range 65–81 years), 52 men and 43 women, were enrolled. The intention‐to‐treat cure rate was 91% (81 of 89; 95% CI = 88–99%) and, 95% (81 of 85; 95% CI = 83–96%) per‐protocol analysis. Compliance was excellent. Mild‐moderate side effects occurred in 27 patients.Conclusions:  Esomeprazole containing quadruple therapy was highly successful for initial eradication of H. pylori in elderly patients.
Effect of Probiotics and Triple Eradication Therapy on the Cyclooxygenase (COX)‐2 Expression, Apoptosis, and Functional Gastric Mucosal Impairment in Helicobacter pylori‐Infected Mongolian Gerbils
Authors: Brzozowski Tomasz , Konturek Peter C. , Mierzwa Marzena , Drozdowicz Danuta , Bielanski Wladyslaw , Kwiecien Slawomir , Konturek Stanislaw J. , Stachura Jerzy , Pawlik Wieslaw W. , Hahn Eckhart G. 
Published: January 2006

AbstractBackground:  Helicobacter pylori infection in Mongolian gerbils is an established experimental model of gastric carcinogenesis that mimics H. pylori‐positive patients developing gastric ulcer and gastric cancer, but the effect of probiotic therapy on functional aspects of this infection remains unknown.Methods:  We compared the effects of intragastric inoculation of gerbils with H. pylori strain (cagA+ vacA+, 5 × 106 colony forming units/ml) with or without triple therapy including omeprazole, amoxicillin, and tinidazol or probiotic bacteria Lacidofil. Histology of glandular mucosa, the viable H. pylori, and density of H. pylori colonization were evaluated. The gastric blood flow was measured by H2‐gas clearance method; the plasma gastrin and gastric luminal somatostatin were determined by RIA and expression of cyclooxygenase (COX)‐2 and apoptotic Bax and Bcl‐2 proteins were evaluated by Western blot.Results:  The gastric H. pylori infection was detected in all animals by histology and H. pylori culture. Basal gastric acid was significantly reduced in H. pylori‐infected animals but not in those with triple therapy or Lacidofil. Early lesions were seen already 4 weeks upon H. pylori inoculation and consisted of chronic gastritis and glandular atypia associated with typical regenerative hyperplasia and increased mitotic activity and formation of apoptotic bodies. The H. pylori infection was accompanied by the fall in gastric blood flow, the marked increase in plasma gastrin, the significant fall in gastric somatostatin levels and Bcl‐2 protein expression, and the rise in expression of COX‐2 and Bax proteins. These mucosal changes were counteracted by the triple therapy and Lacidofil.Conclusions:  H. pylori infection in gerbils, associated with regenerative hyperplasia of glandular structure, results in the suppression of gastric secretion, overexpression of COX‐2, and enhancement in apoptosis and impairment of both, gastric blood flow and gastrin–somatostatin link that were reversed by anti‐H. pylori triple therapy and attenuated by probiotics.
cFLIP expression correlates with tumour progression and patient outcome in non‐Hodgkin lymphomas of low grade of malignancy
Authors: Valente Guido , Manfroi Federica , Peracchio Claudia , Nicotra Giuseppina , Castino Roberta , Nicosia Gabriella , Kerim Simonetta , Isidoro Ciro 
Published: January 2006

SummaryThe present study investigated whether the expression of cellular Fas‐associated death domain‐like interleukin‐1β‐converting enzyme (FLICE) inhibitory protein (cFLIP) conveys prognostic information in non‐Hodgkin lymphomas (NHLs). cFLIP expression was quantified by immunohistochemistry and immunofluorescence in biopsy specimens from 86 NHL patients for whom clinical information was available. NHL malignancy was graded as high/intermediate or low according to the World Health Organization Classification of Lymphoid Neoplasms. cFLIP was positive in 23 of 45 high‐/intermediate‐grade NHLs and in 25 of 41 low‐grade NHLs. Negative expression of cFLIP was associated with the presence of apoptotic cells in the tumour mass, regardless of the histotype and of the malignancy grade. In NHLs positive for cFLIP, 11 of 23 (48%) high‐/intermediate‐grade cases and 18 of 25 (72%) low‐grade cases showed a bad outcome. In NHLs negative for cFLIP, only four of 22 (18%) high‐/intermediate‐grade patients and 12 of 16 (75%) low‐grade patients achieved complete remission. All these correlations were statistically significant. The correlation of cFLIP expression with clinical outcome was independent of therapy, whether or not it included anti‐CD20 antibody (Rituximab). The present findings strongly indicate that cFLIP is a reliable predictor of tumour progression and clinical prognosis in NHLs of low grade of malignancy.
A systematic review of phase‐II trials of thalidomide monotherapy in patients with relapsed or refractory multiple myeloma
Authors: Glasmacher Axel , Hahn Corinna , Hoffmann Florian , Naumann Ralph , Goldschmidt Hartmut , von Lilienfeld‐Toal Marie , Orlopp Katjana , Schmidt‐Wolf Ingo , Gorschlüter Marcus 
Published: January 2006

SummaryThe activity of thalidomide in relapsed or refractory multiple myeloma is widely accepted but not yet demonstrated in a randomised‐controlled trial. A systematic review of the published clinical trials of these patients could reduce the possible bias of single phase‐II studies. A systematic search identified 42 communications reporting on 1674 patients. Thirty‐two trials used an escalating dosing regimen and four a fixed dose regimen (one dose with 50 mg/d, three doses with 200 mg/d). The target dose in the dose escalating trials was 800 mg/d in 17 trials, 400–600 mg/d in 10 and 200 mg/d in one trial. The intention‐to‐treat population for efficacy was 1629 patients with a median age of 62 years. The complete and partial (>50% reduction in monoclonal protein) response rate was 29·4% (95%‐confidence interval, 27–32%). The rates for minor responses or stable disease were 13·8% (12–16%) and 11·0% (9–13%). Progressive disease was reported in 9·9% (8–11%). The median overall survival from all trials was reported at 14 months. Severe adverse events (grade III–IV) included somnolence 11%, constipation 16%, neuropathy 6%, rash 3%, thrombo‐embolism 3%, cardiac 2%. In conclusion, thalidomide monotherapy achieved complete and partial responses in 29·4% of patients with relapsed or refractory multiple myeloma.
Human α2‐globin nonsense‐mediated mRNA decay induced by a novel α‐thalassaemia frameshift mutation at codon 22
Authors: Pereira Francisco J. C. , Silva Maria do Céu , Picanço Isabel , Seixas Maria T. , Ferrão Anabela , Faustino Paula , Romão Luísa 
Published: January 2006

SummaryWe describe a novel α‐thalassaemia determinant in a 3‐year‐old girl presenting a mild microcytic and hypochromic anaemia, and normal haemoglobin A2 level. Molecular studies revealed heterozygosity for a novel microdeletion (−C) at codon 22 of the α2‐globin gene. As the frameshift mutation generates a premature translation termination codon at position 48/49, we investigated the effect of the nonsense codon on the α2‐globin gene expression. Although it does not affect RNA splicing, the premature nonsense codon induces accelerated mRNA degradation. To our knowledge, this is the first time the nonsense‐mediated mRNA decay has been reported to occur in human α‐globin mRNA.
Gene expression of pulmonary cytokines after sevoflurane or thiopentone anaesthesia in pigs
Authors: Takala R. S. K. , Soukka H. , Salo M. S. , Kirvelä O. , Kääpä P. , Aantaa R. 
Published: January 2006

Background:  Volatile anaesthetics have diverse inflammatory effects on the lungs. They increase gene expression of some pro‐inflammatory cytokines in alveolar macrophages whereas in alveolar type II cells they seem to decrease secretion and gene expression of pro‐inflammatory cytokines. We have previously detected increased leukotriene C4, nitrate and nitrite concentrations in bronchoalveolar lavage fluid after sevoflurane anaesthesia. In the current study, we measured gene expression of inflammatory cytokines in the lung tissue and plasma concentrations of cytokines in pigs after thiopentone or sevoflurane anaesthesia.Methods:  Sixteen pigs were randomly selected to receive either a continuous thiopentone infusion (control group, n = 8) or sevoflurane (n = 8) at 4.0% inspiratory concentration (1.5 MAC) in air for 6 h. Tissue samples were collected at the end of the study for measurement of gene expression of inflammatory cytokines. Blood samples were collected during anaesthesia for measurement of plasma cytokine concentrations.Results:  Compared with thiopentone anaesthesia, lower gene expression of tumour necrosis factor‐α (TNF‐α) and interleukin‐1β (IL‐1β) in lung tissue was observed after sevoflurane anaesthesia. Of measured cytokines IL‐1β, TNF‐α, IL‐6, IL‐8 and IL‐10 only plasma concentrations of IL‐6 could be measured during the study without a difference between the groups.Conclusion:  Lower gene expression of TNF‐α and IL‐1β was found in the intact porcine lung tissue after sevoflurane anaesthesia compared with thiopentone anaesthesia. Clinical significance of this finding is unknown.