bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Artificial Reefs on the Macrobenthic Community Structure Through Functional Trait Analysis
Authors: Wang Jiao , Jiang Shaoyu , Sun Debin , Yi Fan , Liu Hang , Chen Linlin , Li Baoquan , Chen Jing 
Published: February 2025

ABSTRACTArtificial reefs enhance marine biodiversity by increasing habitat complexity and heterogeneity, which in turn provide living space for various marine species. Macrobenthos, an important component of aquatic ecosystems, with limited mobility and sensitivity to environmental changes, commonly serve as indicators of aquatic ecosystem health. This study, conducted in the summer and autumn of 2022 in Sishili Bay (SB) and the Changdao Archipelago (CA), Yantai, China, aimed to evaluate the effects of artificial reefs on macrobenthic community structure using functional trait analysis. The nonmetric multidimensional scaling (nMDS) analysis based on species abundance and occurrence frequency did not reveal any clear separation between areas inside and outside the artificial reefs. However, the construction of artificial reefs increased the species number of macrobenthos, especially the Mollusca species. Feeding type, a key functional trait, distinguished areas inside and outside artificial reefs, with significant spatial and temporal variations. The key species driving differences between reef and nonreef areas include the carnivorous Lumbrineris longifolia and Glycinde bonhourei and the omnivorous Eriopisella sechellensis and Sigambra bassi, which were pivotal in the observed variations in relative abundance. Functional richness (FRic) and species richness (S) showed consistent patterns of variation, both contributing to improving the community stability in summer. However, in autumn, community stability was reduced by the extraordinary dominance of a single species. Multiple environmental factors rather than a single one significantly influenced species distribution and diversity, reflecting the complex interplay of reefs and seasonal dynamics. This study used functional trait analysis to assess the impact of artificial reefs on macrobenthic community structure, offering a more effective measure than species‐level assessments.
Central Sensitivity Symptoms and Autistic Traits in Autistic and Non‐Autistic Adults
Authors: Grant Sarah , Norton Sam , Hoekstra Rosa A. 
Published: February 2025

ABSTRACTCentral sensitivity syndromes (CSSs) are a group of health conditions thought to include an underlying sensitisation of the central nervous system. Evidence suggests autistic adults experience poorer physical health than the general population and are more likely to have a CSS. This study examined CSS diagnoses and symptoms in autistic and non‐autistic adults, to determine whether CSS symptoms were related to autistic traits, mental health, sensory sensitivity, age or gender. Participants included 534 adults with clinical diagnoses of autism, CSS, both diagnoses or neither (i.e., comparison group), who were recruited through social media, support groups and institutional affiliations. Participants completed online self‐report validated questionnaires, including the Autism Spectrum Quotient (AQ), Central Sensitization Inventory (CSI), Sensory Perception Quotient (SPQ), and the PHQ‐9 and GAD‐7. Autistic people without a diagnosed CSS reported significantly more CSS symptoms than the comparison group, with a mean score above the clinical cut‐off. Non‐autistic participants with a CSS had significantly more autistic traits than the comparison group. Autistic people with a CSS reported the most sensory sensitivity, with autism only and CSS only groups reporting similar levels of sensory sensitivity and all diagnostic groups reporting more sensory sensitivity than the comparison group. Sensory sensitivity, anxiety, autistic traits, age and gender were all significant predictors of CSS symptoms. The overlap in symptoms between autistic individuals and those with CSS suggests diagnostic overshadowing and possible under‐diagnosis or misdiagnosis. Furthermore, these symptoms may exacerbate or mask one another. Notwithstanding potential limitations of representativeness and selection bias, increased awareness of the association between autistic traits and CSS symptoms is important for clinicians to improve diagnostic accuracy and treatment.
Peptide Fluorescent Probes Based on Aggregation‐Induced Emission for the Detection of Ni2+ and Zn2+ in Different Buffer Systems
Authors: Wang Dajiang , Ji Ping , Liu Xiaomei , Hu Xin 
Published: February 2025

ABSTRACTHeavy metal contamination has emerged as a significant global environmental concern. The contamination of Ni2+ and Zn2+ has attracted increasing attention, not only because of the pollution it causes but also because of the potential risks it poses to human health. It is of great importance to explore sensitive and rapid analytical methods for the accurate detection of Ni2+ and Zn2+. This paper presents the design and synthesis of a peptide fluorescent probe, TPE‐HN (TPE‐Pro‐Trp‐His‐Glu‐Phe‐Gln‐NH2), coupled with a peptide to tetraphenylethylene (TPE). The aggregation‐induced emission (AIE) effect has been employed to construct a rapid ‘turn‐on’ assay for Ni2+and Zn2+ peptide fluorescent probes. The probe is capable of qualitatively detecting Ni2+ and Zn2+ in different buffer systems and can be distinguished by changes in buffer systems. The limit of detection for Ni2+ and Zn2+ in a 15% buffer solution was 9.613 mM (R2 = 0.9924), whereas the limit of detection for Ni2+ in a 20% buffer solution was 1.215 mM (R2 = 0.9922). The probe exhibits high sensitivity, high cell permeability and low biotoxicity, rendering it suitable for live‐cell imaging under biological conditions. This demonstrates that TPE‐HN is capable of detecting Ni2+ and Zn2+ in biological environments.
Ultra‐High‐Performance Liquid Chromatography Combined With Mass Spectrometry Detection Analytical Method for the Determination of N‐Nitrosoduloxetine in Duloxetine HCl
Authors: Marlés‐Torres Anna , Bessa‐Jambrina Sergio , Galán‐Rodríguez Cristóbal 
Published: February 2025

ABSTRACTIn the present study, one simple, fast, feasible, and sensitive ultra‐high‐performance liquid chromatography combined with mass spectrometry detection method, using electrospray ionization, is proposed. This method was developed and validated for the determination of N‐nitrosoduloxetine content in Duloxetine HCl active pharmaceutical ingredient in 11 min employing a simple preparation method for both reference and sample solutions. The proposed method was validated as per regulatory guidelines. Acquity HSS T3 (3.0 × 100 mm, 1.8 μm) column and formic acid 0.1% in water combined with acetonitrile were used for chromatographic separation. The limit of detection and the limit of quantification—reporting threshold—were found to be 0.7 and 70 ppb, respectively. The trueness and precision of the method have been demonstrated in the working range, giving values of recovery within the range of 100%–110%, and the regression coefficients (R) were found to be in the range of 0.9990–0.9991. This method could be used for controlling N‐nitrosoduloxetine content in duloxetine HCl drug substance batches manufactured at Moehs group.
Chiral Lead Halide Perovskites in Action: Unlocking Enantiomer Separation Puzzle
Authors: Singh Pallavi , Mukherjee Rudra , Kumar Anil 
Published: February 2025

ABSTRACTEffective enantiomer separation is vital in many important sectors like pharmaceuticals, agrochemicals, food safety, and biomedical imaging, yet conventional methods are costly, slow, and chemical intensive. This has sparked interest in exploring novel materials like chiral lead halide perovskite nanocrystals to address these challenges. This newly emerging chiral material combines the superior properties of traditional halide perovskites with the unique attributes of chirality, resulting in distinct optoelectronic behaviors. This perspective provides a discussion on future research opportunities in the usage of these chiral LHP NCs for enantiomeric recognition and separation. LHPs exhibit extraordinary photophysical properties, easier surface functionalization, range of bonding interactions, and high surface area to volume ratio that can be used for detecting enantiomers. To the best of our knowledge, the use of chiral halide perovskites for the chiral discrimination of enantiomers has been scarcely reported, presenting a novel opportunity to explore their potential in enantiomeric recognition and separation.
Bioanalytical Tandem Mass Spectrometry Method for Precise Measurement of Tranexamic Acid in Human Plasma With Unveiling Methodological Advancement: Green Assessment With Advanced Metrics
Authors: Kollapareddy Anil , Sayyad Kousrali , Kowtharapu Leela Prasad , Konduru Naresh , Mondal Tanmoy , Varkolu Mohan , Gundekari Sreedhar 
Published: February 2025

ABSTRACTFor the control of excessive blood loss occurring from major trauma, postpartum bleeding, surgery, or hereditary angioedema, antifibrinolytic compound tranexamic acid (TXM) is highly efficient in controlling blood loss by reversibly binding with on lysine receptor sites. A selective and sensitive method has been developed and validated for the quantitation of TXM in human plasma using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC‐MS/MS). Tranexamic acid‐D2 (TXM‐D2) was used as internal standard (ISTD) to minimize the errors in TXM quantification. TXM quantification was performed with positive polarity mode using a Shimadzu high performance liquid chromatography coupled with AB‐SCIEX API‐4000 tandem mass spectrometer. A Zorbax Eclipse C18 (150 × 4.6 mm, 5 μ) column was used for the TXM quantification, 8‐mM ammonium formate buffer with 0.1% formic acid ionization enhancer was used as a mobile phase‐A, and acetonitrile was used as a mobile phase‐B. (38:62) v/v portion of mobile phases A and B selected to elute the TXM and TXM‐D2 with the flow rate of 0.8 mL/min. An electrospray ionization (ESI) technique was selected for detection of TXM in the human plasma. Linearity was assessed in the concentration range from 75 to 15,000 ng/mL by using least squares of weighting factor linear regression (1/X2).
Simultaneous Determination and Pharmacokinetics of Three Steroidal Alkaloids in Rat Plasma by UHPLC–MS After Oral Administration of Three Different Preparations of Fritillaria ussuriensis Maxim Bulb
Authors: Wu Haibo , Yang Chunjuan , Chen Jingnan , Li Sen , Wei Yuxin , Wang Meng , Li Xinlan , Wang YanYan , Kuang Haixue , Wang Zhibin 
Published: February 2025

ABSTRACTPharmacokinetic studies can provide a method for optimizing preparations. In this study, three most abundant compounds from Fritillaria ussuriensis bulb were isolated, and a selective, sensitive new analytical method was developed. This method aims for the simultaneous determination of peimisine‐3‐O‐β‐D‐glucopyranoside (1), karelinine (2), and peimisine (3) in rat plasma after oral administration of F. ussuriensis bulb powder (FUP), total alkaloid extract (FUE), and its nanodispersion preparation (FUN) using UHPLC–MS/MS. The chromatographic separation was performed on the UHPLC Waters BEH C18 column. The pharmacokinetic results showed that among three groups, 1 and 3 exhibited slower absorption and elimination processes when rats were orally administered with FUP and FUE. Steroidal alkaloids 1 ~ 3 from FUN reached peak concentrations within 3 h. Compared with FUE and FUP, the absorption and elimination rates were accelerated, and the area under the curve (AUC) significantly increased. In this study, the pharmacokinetic characteristics of 1 ~ 2 were first detected and calculated parameters in rat plasma when orally administered in different dosage forms. And a new nanodispersion preparation, FUN, has been developed. FUN has better absorption efficiency and higher bioavailability compared with traditional powder oral administration.
A Validated Liquid Chromatography–Tandem Mass Spectrometry Assay for the Determination of NX‐5948 in Beagle Dog Plasma: Application to a Pharmacokinetic Study
Authors: Wang Fang , Sun Wenfei , Ma Zheheng , Chu Furui 
Published: February 2025

ABSTRACTProteolysis targeting chimera (PROTAC) has been developed and currently enjoys widespread interest among the field of pharmaceutical manufacturing in recent decades. NX‐5948 is an orally active PROTAC Bruton tyrosine kinase degrader, which shows anti‐inflammatory and antitumor activities. In this study, a simple and fast bioanalytical method for the quantification of NX‐5948 in beagle dog plasma was developed using liquid chromatography–tandem mass spectrometry (LC‐MS/MS). A full method validation was performed according to regulatory guidelines. For the quantification, [M + H]+ was formed using an electrospray ionization (ESI) source in the positive ion mode, and selective reaction monitoring (SRM) was employed using a triple quadrupole mass spectrometry. The monitored precursor‐to‐product transitions were m/z 807.5 > 790.5 for NX‐5948 and m/z 812.4 > 452.1 for internal standard. A linear response was obtained at the concentration range of 0.5–200 ng/mL, with correlation coefficient > 0.99. The interday accuracy ranged from −9.03% to 5.99% (RSD < 7.84%), and the intraday accuracy from −5.15% to 8.56% (RSD < 6.90%). NX‐5948 was demonstrated to be stable under the present storage conditions. After validation, the method was successfully applied for the quantification of NX‐5948 in beagle dog plasma after oral and intravenous administration of the NX‐5948.
Global Trends of Early, Middle, and Late‐Onset Lung Cancer From 1990 to 2021: Results From the Global Burden of Disease Study 2021
Authors: Li Zongyuan , Yu Cheng , Hao Jianqi , Luo Nanzhi , Peng Haoning , Zhang Jian , Pu Qiang , Liu Lunxu 
Published: February 2025

ABSTRACTBackgroundAlthough the global burden of lung cancer has generally declined in recent decades, the variation in onset age‐related trends remains insufficiently explored. In the current study, we aimed to systematically evaluate the most update temporal trends in incidence, mortality and DALYs of early, middle, and late‐onset lung cancer (EOLC, MOLC, and LOLC) from 1990 to 2021, with stratifications of gender, location, and socio‐demographic development.MethodsWe retrieved cross‐sectional data from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021. The global, regional, and national burden of lung cancer from 1990 to 2021 was evaluated primarily by age‐standardized rates of incidence (ASIR), mortality (ASMR), and DALYs (ASDR). Joinpoint regression analysis was employed to assess temporal trends and turning point years. Frontier analysis was applied to examine the lowest achievable DALYs, and cross‐country inequalities were evaluated sing the slope index of inequality (SII) and concentration index. We also forecasted the burden from 2022 to 2035.ResultsThe global ASIR of EOLC decreased from 4.81 per 100,000 in 1990 to 3.13 in 2021 (AAPC: −1.38, 95% confidence interval [CI]: −1.53 to −1.22, p < 0.001), with a steeper decline in males (AAPC: −1.79) compared to females (AAPC: ‐0.63). MOLC incidence also declined from 92.77 to 72.47 per 100,000 (AAPC: ‐0.81, 95% CI: −0.9 to −0.73, p < 0.01), while LOLC demonstrated a slight increase from 195.39 to 225.8 per (AAPC: 0.43, 95% CI: 0.37 to 0.5, p < 0.01). Notably, LOLC in females showed a consistent rise in incidence rate (AAPC: 1.13, 95% CI: 1.05 to 1.21, p < 0.01). In contrast to EOLC and MOLC, 11, 10, and 9 out of 21 GBD regions showed a rising trend for ASIR, ASMR, and ASDR of LOLC, respectively. East Asia showed the steepest increase in ASIR (from 229.26 in 1990 to 375.90 in 2021, AAPC = 1.6, 95% CI: 1.31 to 1.89, p < 0.001) of LOLC. Moreover, according to socio‐demographic index (SDI) quintiles, the middle SDI region demonstrated the largest rise in ASIR of LOLC. Frontier analysis revealed that countries with higher SDIs had a greater capacity for reducing lung cancer burdens. Cross‐country inequalities of lung cancer burden in females were found to improve much slower than in males. The projections implied that, although lung cancer would generally decline in the next decade, the incidence, mortality, and DALY rates of LOLC in females might remarkably increase.ConclusionsThe global incidence, mortality, and DALY rates of lung cancer showed a general decline from 1990 to 2021. However, concerning trends of LOLC burden, especially among females and in specific regions or countries, were observed in this study. This study could help to guide more targeted prevention and intervention strategies for lung cancer control.
A Practical Approach for Polarity and Quantity Controlled Assembly of Membrane Proteins into Nanoliposomes
Authors: Zhang Shiwei , Lin Peng , Komatsubara Futa , Nakata Eiji , Morii Takashi 
Published: February 2025

AbstractBiological membranes achieve selectivity and permeability through protein transporters and channels. The design of artificial compartments with permeable membranes is essential to facilitate substrate and product transfer in enzymatic reactions. In this study, an E. coli outer membrane protein OmpF fused to a modular adaptor was integrated onto a DNA origami skeleton to control the number and polarity of the OmpF trimer. DNA origami skeleton‐guided nanoliposomes reconstituted with functional OmpF exhibit pH‐responsiveness and size‐selective permeability. This approach highlights the potential to construct artificial compartments that incorporate membrane proteins of defined number and polarity, allowing tunable substrate fluxes.
Palladium‐Catalyzed Selectivity Controlled Highly Efficient Carbonylative Synthesis of Isoquinoline‐1,3(2H,4H)‐diones and Indanones
Authors: Xu Ren‐Rui , Kuai Chang‐Sheng , Wu Xiao‐Feng 
Published: February 2025

AbstractCompared to the methods that rely on complex and structurally unique substrates for the synthesis of heterocyclic compounds through intra‐ and intermolecular cyclization, strategies starting from simple and readily available substrates, combined with finely tuned catalytic system to achieve different chemoselectivity, offer significant advantages in terms of efficiency, cost‐effectiveness, and versatility. Herein, we present a palladium‐catalyzed carbonylation strategy that enables the selective and efficient synthesis of isoquinoline‐1,3(2H,4H)‐dione and indanone derivatives via distinct reaction pathways. Notably, the chemoselectivity was not determined by the differences in reactivity at the reaction sites but instead arises from the generation of distinct active palladium species under nearly identical reaction conditions, which subsequently react on the same reaction site but lead to different products.
Pt Nanoparticles Loaded on Bimetallic Porphyrinic MOF for Selective Hydrogenation Reactions
Authors: Zhao Meihua , Li Zhuo , Chen Jianfa , Guo Danyu , Nie Zhongfen , Fang Ruiqi , Li Yingwei 
Published: February 2025

AbstractMetal nanoparticles (MNPs) loaded on metal‐organic frameworks (MOFs) show promising catalytic performances in various reactions, but finely tuning the electronic properties of MNPs remains challenging. Herein, metal NP‐MOF synergy has been finely regulated by incorporating Pt NPs into PCN‐224 (FeCo). The as‐prepared Pt/PCN‐224 (FeCo) exhibited high catalytic activity in the hydrogenation of various carbonyl substrates. Detailed characterizations and mechanistic studies revealed that the Fe/Co sites of the ligands can effectively tune the electronic properties of Pt NPs, which achieved promoted capability to activate H2. The atomic hydrogen (H*) produced by Pt sites mainly migrated to the Co sites to promote the hydrogenation process via hydrogen spillover, thereby enhancing the catalytic activity. This work is devoted to a promising strategy to optimize the catalytic performance of MOF‐based nanomaterials by incorporating metal NPs in the thermocatalytic systems.
Physical characteristics and controlling factors of tight sandstone gas reservoir: A case study of upper Palaeozoic tight sandstone in Shenmu Mizhi area
Authors: Wu Yufeng , Zhang Yuting , Fu Guomin 
Published: February 2025

AbstractThe Shenmu Mizhi area has multi‐layered gas bearing formations, but the pore structure of the tight reservoirs in the area is complex. In this paper, first, the source characteristics of the reservoir in the study area were analyzed, and the reservoir petrological characteristics such as clastic characteristics, rock fragment characteristics, and reservoir filling material characteristics were explored. Second, the pore structure and throat characteristics of the study area were analyzed based on the genesis and distribution of pores, combined with morphology and structure. Third, the pore structure characteristics were elucidated, and the frequency distribution of porosity and permeability of typical rock cores were characterized. Results show the following: (a) The main types of pores are rock debris dissolution pores, followed by intergranular pores. The average facial recognition rate for a Qian 5 section is 2.42%. (b) Except for the Qian 5 section, the average mercury removal efficiency of all other layers is greater than 30%. (c) The porosity distribution range of the Qian 5 section of the Shiqianfeng Formation is 2.8% to 17.3%, with an average value of 8.5% and a main value range of >12%; The distribution range of permeability is 0.001–287.5 × 10−3 μm2, with an average value of 3.31 × 10−3 μm2 and a main value range of >1 × 10−3 μm2.
Prevalence of Epstein‐Barr Virus, Cytomegalovirus, and Periodontopathic Bacteria in Patients With Periodontitis: A Case–Control Study
Authors: Herrero‐Sánchez Alicia , Haroyan‐Darbinyan Evelina 
Published: February 2025

ABSTRACTObjectivesOpportunistic viruses such as cytomegalovirus (CMV) and Epstein‐Barr virus (EBV) have been detected in gingival crevicular fluid (GCF) and saliva of patients with periodontal disease. However, the relationship between herpesviruses and periodontitis remains obscure. The aim of this case–control study was the detection and association of CMV and EBV with periodontitis.Material and MethodsForty‐eight adults were included in this study: 24 patients with periodontitis (CP) and 24 periodontally healthy individuals (HS). All patients underwent periodontal examination, including probing depth (PD), clinical attachment loss (CAL), plaque index (PI), and bleeding on probing (BOP). Subgingival biofilm samples were collected from all patients and real‐time PCR was performed for viral and bacterial detection. The odds ratio (OR) was calculated, and the chi‐squared test or Fisher's exact test was performed to analyze the significant differences.ResultsEBV was detected only in one healthy patient meanwhile no CMV was found. No statistically significant differences were found between the periodontal clinical parameters of EBV‐positive patients and the negative ones: PI (p = 0.090), PD (p = 0.857), CAL (p = 0.801), and BOP (p = 0.104). Except for Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Prevotella intermedia (Pi), and Treponema denticola (Td) showed a statistically significant association (p < 0.001) with the clinical periodontal parameters. Aa presence was not statistically associated with periodontal sites (p < 0.296). Tf and Pg were the most frequently detected periodontopathic bacteria in the CP group (91.7% sites).ConclusionThe present case–control study showed that the prevalence of EBV and CMV did not show significant differences between the groups evaluated in the Spanish population.