bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome analysis reveals resveratrol promotes lipid synthesis and induces apoptosis in Siberian sturgeon
Authors: Yang Shiyong , Yan Chaozhan , Pang Xiaojian , Shao Wuyuntana , Xu Zihan , Li Datian , Xu Wenqiang , Zhang Zhehua , Su Boru , Li Yunkun , Wu Jiayun , Huang Xiaoli , Luo Wei , Du Xiaogang 
Published: March 2024

Abstract

Resveratrol, known for its antioxidant properties and ability to regulate glycolipid metabolism, has been revealed its benefit effects on the liver health of sturgeon. However, further investigation is needed to understand the specific mechanism of resveratrol's impact. In this study, we explore how resveratrol affects the liver of Siberian sturgeon at a specific concentration. The results showed that resveratrol treatment led to a significant increase in apoptosis after hepatic lipid synthesis, but did not activate the antioxidant NRF2/KEAP1 pathway or enhance antioxidant enzyme activity. Transcriptomics analysis also revealed significant changes in regulatory pathways related to glycolysis, including the PPAR signaling pathway, Insulin signaling pathway, Fatty acid biosynthesis, and Glycolysis/Gluconeogenesis. These findings highlight the close relationship between resveratrol and glycolipid metabolism. Moreover, the study demonstrated that high concentrations of resveratrol can have physiological toxicity to the liver of Siberian sturgeon, providing valuable insights for future research on fish feed additives dosage.
Causal Models and Prediction in Cell Line Perturbation Experiments
Authors: Long James , Yang Yumeng , Shimizu Shohei , Pham THONG , Do Kim-Anh 
Published: March 2024

Abstract

In cell line perturbation experiments, a collection of cells is perturbed with external agents and responses such as protein expression measured. Due to cost constraints, only a small fraction of all possible perturbations can be tested in vitro. This has led to the development of computational models that can predict cellular responses to perturbations, without having to actually perform the experiments in a wet lab. A central challenge for these models is to predict the effect of new, previously untested perturbations that were not used in the training data. Here we propose causal structural equations for modeling how perturbations effect cells. From this model, we derive two estimators for predicting responses: a Linear Regression (LR) estimator and a causal structure learning estimator that we term Causal Structure Regression (CSR). The CSR estimator requires more assumptions than LR, but can predict the effects of drugs that were not applied in the training data. Next we present Cellbox, a recently proposed system of ordinary differential equations (ODEs) based model that obtained the best prediction performance on a Melanoma cell line perturbation data set (Yuan et al., 2021). We derive analytic results that show a close connection between CSR and Cellbox, providing a new causal inter- pretation for the Cellbox model. We compare LR and CSR/Cellbox in sim- ulations, highlighting the strengths and weaknesses of the two approaches. Finally we compare the performance of LR and Cellbox on the benchmark Melanoma data set. We find that the LR model has comparable or slightly better performance than Cellbox.
Regional prediction of deoxynivalenol contamination in spring oats in Sweden using machine learning
Authors: Wang Xinxin , BÖRJESSON Thomas , Wetterlind Johanna , Fels-Klerx HJ van der 
Published: March 2024

Abstract

Weather conditions and agronomical factors are known to affect Fusarium spp. growth and ultimately deoxynivalenol (DON) contamination in oat. This study aimed to develop predictive models for the contamination of spring oat at harvest with DON on a regional basis in Sweden using machine-learning algorithms. Three models were developed as regional risk-assessment tools for farmers, crop collectors, and food safety inspectors, respectively. Data included weather data from different oat growing periods, agronomical data, site-specific data, and DON contamination data from the previous year. The region, year, spring oat variety, type of cultivation (organic or not) and if the oat is intended for feed or food - was used as input to predict DON contamination for entries into classes of low (< 500 µg/kg), medium (≥ 500 µg/kg, and < 1000 µg/kg), and high (≥ 1000 µg/kg). A random forest (RF) algorithm was applied to train the models. Results showed that: 1) RF models were able to predict DON contamination at harvest with a total classification accuracy of minimal 0.72, over the years 2012-2019, and above 0.90 in the years 2016-2017, however not for individual years not included in the training of the models (external validation); 2) good predictions could already be made in June but using weather variables in the full growing season could improve the model’s robustness; 3) weather variables were the most important for predicting DON contamination, but adding agronomical and site-specific factors to weather variables as model inputs could improve the overall model performance; 4) rainfall, relative humidity, and wind speed in different oat growing stages, followed by crop variety and elevation were the most important features for predicting DON contamination in spring oats at harvest. In future studies, it might be of interest to explore whether including data for other agronomic variables, such as fertilization, irrigation, and pest control, as well as satellite image data could further improve the model performance.
Bacteria Invade the Brain Following Sterile Intracortical Microelectrode Implantation
Authors: Capadona Jeffrey , Hoeferlin George , Grabinski Sarah , Druschel Lindsey , Duncan Jonathan , Burkhart Grace , Weagraff Gwendolyn , Lee Alice , Hong Christopher , Bambroo Meera , Olivares Hannah , Bajwa Tejas , Memberg William , Sweet Jennifer , Hamedani Hoda Amani , Acharya Abhinav , Hernandez-Reynoso Ana , Donskey Curtis , Jaskiw George , Chan Ricky , Ajiboye A. , von Recum Horst , Zhang Liangliang 
Published: March 2024

Abstract

Brain-machine interface performance is largely affected by the neuroinflammatory responses resulting in large part from blood-brain barrier (BBB) damage following intracortical microelectrode implantation. Recent findings strongly suggest that certain gut bacterial constituents penetrate the BBB and are resident in various brain regions of rodents and humans, both in health and disease. Therefore, we hypothesized that damage to the BBB caused by microelectrode implantation could amplify dysregulation of the microbiome-gut-brain axis. Here, we report that bacteria, including those commonly found in the gut, enter the brain following intracortical microelectrode implantation in mice implanted with single-shank silicon microelectrodes. Systemic antibiotic treatment of mice implanted with microelectrodes to suppress bacteria resulted in differential expression of bacteria in the brain tissue and a reduced acute inflammatory response compared to untreated controls, correlating with temporary improvements in microelectrode recording performance. Long-term antibiotic treatment resulted in worsening microelectrode recording performance and dysregulation of neurodegenerative pathways. Fecal microbiome composition was similar between implanted mice and an implanted human, suggesting translational findings. However, a significant portion of invading bacteria was not resident in the brain or gut. Together, the current study established a paradigm-shifting mechanism that may contribute to chronic intracortical microelectrode recording performance and affect overall brain health following intracortical microelectrode implantation.
Synthesis, characterization and detection of amoxicillin on 𝜷-AgVO3 modified carbon paste electrode and investigation of adsorption by DFT approach
Authors: Atika Ayad , HERMOUCHE lina , ABDOUNI ibtissam El , Benhsina Elhassan , HAJJAJI Souad EL 
Published: March 2024

Abstract

The weighty industrialization, rapid urbanization and the changing lifestyle have been considered as a worldwide threat, these human activities produce a huge pollutant element especially in water such as antibiotics, heavy metals etc.. Hence, the sensing and quantification of trace pollutants in aquatic system have been becoming a necessity tool to develop. In this work, we synthesis the silver metavanadate AgVO3 through solid state reaction, the obtained powder was analyzed using X-ray diffraction, infrared spectroscopy and scanning electronic microscopy to check the structure and purity of the silver metavanadate. The elaborated compound as a modifier of carbon paste electrode to investigate the sensing of amoxicillin in aqueous solution by means of square wave voltammetry. The effect of electrochemical and chemical parameters on the current intensity was optimized. Under optimized conditions, the prepared electrode had a detection limit of 0.731µM. The interaction between amoxicillin molecule and AgVO3 surface was also investigated, which shows spontaneous adsorption process.
In vitro fertilization and embryo transfer is an independent risk factor of the placenta accreta spectrum in the termination of pregnancy during the mid-trimester：a retrospective cohort study on perinatal management
Authors: Li Si , Liu Mancheng , Zhou Li , Zhao Yunxu , Guo Fei , Gao Yu , Gou Chenyu 
Published: March 2024

Abstract

BACKGROUND
The most common aetiology of placenta accreta spectrum (PAS) is a defect in the endometrial-myometrial interface. The incidence and characteristics of the PAS among women with termination of pregnancy after the second trimester are unknown. This study aimed to determine the effect of in vitro fertilization and embryo transfer (IVF-ET) on patients with termination of pregnancy during the mid-trimester and the risk factors of placenta accreta spectrum among this population.
METHOD
This was a retrospective observational study of women who underwent induced abortion or termination of pregnancy in the second trimester from January 2013 to October 2023 in the Sixth Affiliated Hospital of Sun Yat-sen University. A total of 230 patients were age-matched and divided into a spontaneous conception group and an in vitro fertilization and embryo transfer group to compare the difference in incidence of placenta accreta spectrum and their outcomes during follow-up. The primary outcome was the morbidity of the placenta accreta spectrum.
RESULTS
Our results indicated that women with IVF-ET had a significantly higher risk of suffering PAS (29.6% vs. 13.0%, P = 0.002) as well as morbid placenta accreta spectrum (13.9% vs.5.2%, P = 0.039) during abortion. The amount of postpartum bleeding [200(100.0-520.0) ml vs. 140.0(80.0-240.0)ml, P = 0.004], the rate of postpartum haemorrhage (24.3% vs.11.3%, P = 0.010), and the time of retained products of conception > 4 weeks (40.4%vs.24.8%, P = 0.030) were significantly higher in the IVF-ET group than in the spontaneous conception group. IVF-ET (odds ratio = 2.42, P = 0.027), a hysteroscopy history(odds ratio = 2.67, P = 0.028), and cryopreserved embryo transfer (odds ratio = 4.26, P < 0.001) were independent risk factors for PAS during abortion.
CONCLUSION
Patients with IVF-ET, especially cryopreserved embryo transfer, had a significantly high risk of PAS and postpartum haemorrhage during the abortion of mid-trimester, suggesting that these patients need more attention to avoid severe complications and preserve fertility during labour.
Total Self-BAT scores associated with choice of heavy menstrual bleeding treatment
Authors: Eising Heleen P. , Punt Marieke C. , Grimberg Evelyn , Schermer Tjard R. , Leemans Jaklien C. , Bongers Marlies Y. 
Published: March 2024

Abstract
Background
Heavy menstrual bleeding (HMB) is frequently the indicating symptom of an underlying bleeding disorder. The self-administered bleeding assessment tool (self-BAT) provides an overview of the bleeding history and indication of a bleeding tendency. Research about the association between a bleeding tendency and choice of HMB treatment is scarce.
Methods
The self-BAT was sent to all adult patients with HMB seeking care at a gynecological clinic. Closed questions on medical and surgical HMB management were provided, as well as a free-text question to reflect on personal experiences and opinions concerning HMB management. For clinical assessment of bleeding tendency, the cut-off for an abnormal self-BAT score was ≥5 in young women (18-30 years) or ≥6 for women of 31-52 years.
Results
Overall, 36% (234/650) of eligible women completed the questionnaire. The median total self-BAT score was 6 (interquartile range: 0–17). An abnormal total self-BAT score was more prevalent in women who underwent a hysterectomy compared to those who had not for women age 31-52 years (OR 4.3; 95%CI,1.2; 15.3; p=0.02). Medical therapy (hormonal, tranexamic acid or iron) was more prescribed in women with an abnormal self-BAT score. The most frequently mentioned responses from patients were related to low awareness of the effects of HMB and the need for patient empowerment.
Conclusions
Findings imply that there is a substantial group of patients with HMB who experience a bleeding tendency. This bleeding tendency may influence the choice of HMB treatment like a hysterectomy. Structured bleeding assessment tools could guide optimal diagnostics and treatment for women with HMB.
Surgery for severe pneumocephalus caused by Gorham-Stout disease: a case report and literature review
Authors: Zhang Yiming , Zhang Dong , Qi Yinbao , Wang Lanlan , Liu Xiang , Yue Peidong , Qian Ruobing 
Published: March 2024

Abstract
Background
Gorham-Stout disease (GSD) is an extremely rare bone resorption disease, and there are few reports of GSD affecting the skull and cervical spine. We report a rare case of severe pneumocephalus caused by GSD in the past decade. Satisfactory results were achieved through surgical treatment.
Case presentation:
The patient had been diagnosed with GSD for 14 years, during which the patient's skull began to dissolve from the occipital bone, and the cervical spine was gradually affected. One day before admission, the patient experienced severe headache and vomiting, and a head CT scan showed severe pneumocephalus. After admission, the patient underwent fistula repair surgery. One year after surgery, the patient's condition remained stable.
Conclusion
There is currently no effective unified treatment plan for GSD. Here, we provide a case in which severe pneumocephalus caused by GSD was effectively treated through surgery, which provides treatment ideas for similar patients.
Incident of violence escalation of patients with psychiatric emergencies boarding in the emergency department in the Central Region of Thailand and its association: A prospective observational study
Authors: Khlaisuk Angsana , Seeherunwong Acharaporn , Utriyaprasit Ketsarin , Poungkaew Autchariya , Tongsai Sasima 
Published: March 2024

Abstract
Background
This study aimed to investigate the incidence of violence escalation in psychiatric emergency patients who were admitted to general emergency departments. The study also determined patient and service delivery system factors associated with the survival time of violence escalation in 16 emergency departments in the central region of Thailand. The research design was a prospective observational study, and the study sample includes 507 psychiatric emergency patients over the age of eighteen, selected through stratified random sampling and purposive sampling.
Methods
The study analyzes patient data consisting of demographic data, emergency services used, and clinical characteristics using descriptive statistics. The Kaplan-Meier method was used to estimate the violent escalation curve, and the log-rank test was used to compare violent escalation-free time between the levels of the violent behavior group. Additionally, univariable and multivariable Cox proportional hazard analyses were used to investigate the factors affecting violence escalation.
Results
The results of the study indicate that the incidence of violent escalation of patients with psychiatric emergency boarding in the emergency department was 7.3%, while the incidence rate of violence escalation was 3 per 100 psychiatric emergency patient visits-hours. The factors affecting violent escalation were the violence behavior score at triage (aHR = 2.004; 95% CI: 1.051–3.823) and the nurse competency score (aHR = 0.147; 95% CI: 0.032–0.680).
Conclusions
Assessing the violent behavior of psychiatric emergency patients at triage may assist emergency providers in monitoring patient behavior and providing early intervention to prevent the escalation of violent behavior. Furthermore, training emergency nurses in psychiatric emergency care is required.
Psychological distress associated with suicidal ideation and attempt of people with glaucoma at tertiary eye care clinic, gondar city, Ethiopia
Authors: Andualem Fantahun , Ali Dawed , Tinsae Techilo , Nakie Girum , Demilew Demeke , Tadesse Gebresilassie , Muluye Getachew 
Published: March 2024

Abstract
Background
Suicide is an act of intentionally ending one’s own life with implicit or explicit evidence. The studies done from five countries in Africa (including; Ethiopia) revealed that the pooled prevalence of suicidal ideation was 21.7% and suicidal attempt was 11.06%, whereas, suicidal ideation and attempt were higher in Ethiopia 22.7% and 16.97%, respectively. Suicidal ideation and attempt were associated with various psychological, social and medical conditions. This study aimed to assess the prevalence of suicidal ideation and attempt among adult people with glaucoma and its contributing factors in Gondar city, Ethiopia.
Methods
A cross-sectional study was conducted on 422 glaucoma patients at Tertiary Eye Care Clinic, University of Gondar Comprehensive and Specialized Hospital. The sample size was calculated by using a single population proportion formula and the sample selected by using systematic random sampling method. Suicidal ideation and attempt assessed using the suicidality module of the World Mental Health (WMH) Survey Initiative version of the World Health Organization’s Composite International Diagnostic Interview (CIDI). The data was edited, cleaned, coded and entered into Epi-info version 7 and analyzed by using Statistical Packages for Social Science (SPSS) 25 version. Binary logistic regression was conducted to identify factors associated with suicidal ideation and attempt. The p-value less than 0.05 were considered as statistically significant.
Results
The overall prevalence of suicidal ideation and attempt were 6.4% with 95% CI (4.3–9.2%) and 2.4% with 95% CI (1.1–4.3%), respectively. In multivariate analysis; severe psychological distress, moderate psychological distress, mild psychological distress, and current substance use were identified as a risk factor for suicidal ideation, whereas, lived alone, co-morbid chronic medical disease/diseases and ever substance use were identified as a risk factor for suicidal attempt.
Conclusion
Based on our finding the prevalence of suicidal ideation and attempt was not contemptible. The study also identified factors associated with suicidal ideation and attempt; psychological distress and current substance use were significantly associated with suicidal ideation, whereas living alone, co-morbid chronic disease/diseases and ever substance use were significantly associated with suicidal attempt. Our finding lead, they should be screened and managed in eye care institution.
Robotic-resisted Exercise for Health Promotion in Younger Adults
Authors: Lee Su-Hyun , Kim Eunmi , Kim Jinuk , Kim Dongwoo , Lee Dokwan , Lee Hwang-Jae , Kim Yun-Hee 
Published: March 2024

Abstract

Introduction
Physical inactivity and sedentary behavior both increase the risk of chronic disease and mortality. Regular participation in physical activity and reducing sedentary behavior play important roles in maintaining physical health and disease prevention. The purpose of this study was to investigate the effect of a wearable hip exoskeleton, Bot Fit, on muscle strength, muscle effort, and the kinematics of the pelvis during walking in younger adults.
Methods
We designed three parallel experimental conditions and randomly assigned participants to one of three groups: those assigned to exercise using an interval program of Bot Fit (interval group), those who used a power program of Bot Fit (power group), and a control group who exercised without Bot Fit. A total of 45 young adults participated in 18 exercise-intervention sessions over six weeks, and all participants were assessed at two time points: before and after the 18 exercise sessions. Each assessment evaluated muscle strength, muscle effort, and the kinematics of the pelvis during walking. In addition, the number of steps, distance, energy expenditure, and heart rate for 30 min during the exercise sessions were recorded.
Results
A significant increase in the maximum voluntary contraction (MVC) of the right biceps femoris (BF) was evident in the interval group while significant changes in the MVC of the bilateral BF were seen in the power group showed after Bot Fit exercise. A significant decrease of muscle effort in right BF in the interval group and right lumbar erector spinae and bilateral BF in the power group were also observed. In addition, the symmetry index of pelvic tilt significantly improved in the interval group, and greater exercise volume and intensity in both the interval and power groups compared with the control group were confirmed as measured by the number of steps, distance, energy expenditure, and heart rate.
Conclusion
Results of this study indicate a beneficial effect of the Bot Fit on muscle strength, walking efficiency, and pelvic movement symmetry in younger adults. Personalized exercise programs using different exercise protocol with the Bot Fit may therefore improve the physical health and gait symmetry of younger adults.
Oral administration of Lactobacillus delbrueckii UFV-H2b20 protects mice against Aspergillus fumigatus lung infection
Authors: Andrade Ana Clara Matoso Montuori , Oliveira Nathalia Luisa , Silva Ana Elisa Nolasco e , Vaz Leonardo Gomes , Martins Flávia Rayssa Braga , Lopes Mateus Eustáquio Moura , Torres Lícia , Junior Celso Martins Queiroz , Russo Remo Castro , Santos Liliane Martins , Vieira Leda Quercia , Soriani Frederico Marianetti 
Published: March 2024

Abstract

Methods
we investigated survival, respiratory mechanics, histopathology, colony forming units, cytokines in bronchoalveolar lavage, IgA in feces, efferocytosis, production of reactive oxygen species and the cell population in the mesenteric lymph nodes.
Results
L. delbrueckii induces tolerogenic dendritic cells, IL-10+macrophages and FoxP3+regulatory T cells in mesenteric lymph nodes and increased IgA levels in feces; after infection with A. fumigatus, increased survival and decreased fungal burden. There was decreased lung vascular permeability without changes in the leukocyte profile. There was enhanced neutrophilic response and increased macrophage efferocytosis. L. delbrueckii-treated mice displayed more of FoxP3+Treg cells, TGF-β and IL-10 levels in lungs, and concomitant decreased IL-1β, IL-17A, and CXCL1 production.
Conclusion
our results indicate that L. delbrueckii UFV H2b20 ingestion improves immune responses, controlling pulmonary A. fumigatus infection. L. delbrueckii seems to play a role in pathogenesis control by promoting immune regulation.