bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular manufacturing: Some human resource implications
Authors: Bratton John 
Published: July 2007

AbstractThis article considers some of the human resource management implications of the Japanese manufacturing system. The paper draws on case studies undertaken in England between 1985 and 1988. The paper presents a framework for analyzing the effects of Japanese methods. It is found that the Japanese system resulted in an enhancement of manual engineering skills. Further, it is argued that Japanese practices can coexist alongside traditional industrial relations systems, but because of uncertainty and high dependency on constituents, new organizational control configurations need to be developed.
Extended work cycle assembly—a crucial learning experience
Authors: Engström Tomas , Medbo Lars , Jonsson Dan 
Published: July 2007

AbstractThis paper reports on a crucial learning experience relating to design principles applied in the Volvo Car Corporation final assembly plant in Uddevalla, Sweden. These principles include the concept of holistic learning, specifically through the creation and transformation of complementary, interrelated physical, semantical, and cognitive structures. We report on the learning environment and learning aids, as well as the learning method applied to the assembly of one‐quarter of an automobile performed by one single person. Through the application of this learning concept, it proved possible to dramatically reduce the learning time required for long cycle time assembly work, making cycle times of 2 hours or more a practical proposition for full‐scale automotive manufacturing today. © 1994 John Wiley & Sons, Inc.
Computerized ergonomic analysis in manufacturing: A results‐oriented approach for the practitioner
Authors: Marcotte Andrew J. , Joyce Marilyn 
Published: July 2007

AbstractFor ergonomics to be effectively implemented within an organization, nonspecialists in ergonomics organized in cross‐functional teams need the tools and the knowledge to perform. A worksite analysis and problem‐solving process, assisted by computer programs, can enable the organization's personnel to do the following: identify and prioritize the problems; solve the problems, often with minimal cost; and document results. This paper describes the use of ergonomics checklists and detailed ergonomics task analysis within the system. A case based on the computerized analysis illustrates the method. @ 1995 John Wiley & Sons, Inc.
Integrating safety in manufacturing systems
Authors: Cheng Otto S. , Jiang Bernard C. 
Published: July 2007

AbstractAn integrated safety and manufacturing system (ISM) was developed. With this ISM system, a communication method between the safety system and the manufacturing system by means of a hybrid control strategy was introduced. The hybrid control strategy combined the hierarchical and heterarchical control in order to take advantage of both. Thus, a safety system can have the same weight as that of a manufacturing system, and is no longer an auxiliary part of a system. The safety approach, which was proposed for developing the safety system in ISM system. started by applying open‐loop safety controls and closed‐loop safety controls to machines. Applying a safety training program and personal protection equipment to humans followed. Then, a user‐coded safety module was developed for users who want to integrate the safety concept into their simulation system. Finally, a simulated application example illustrated the compatibility of the safety module and the differences of a system cell before and after integration with the safety system. The simulation results, from throughput, utilization, and safety aspects, are discussed. @ 1995 John Wiley & Sons, Inc.
Regulation of c‐Met signaling by the tetraspanin KAI‐1/CD82 affects cancer cell migration
Authors: Takahashi Miho , Sugiura Tsuyoshi , Abe Masakazu , Ishii Koutaro , Shirasuna Kanemitsu 
Published: July 2007

AbstractIt has been proposed that the metastasis suppressor CD82/KAI‐1, which is a member of the tetraspanin superfamily, regulates biological activity by associating with cell surface receptors or proteins. We show a novel association between CD82 and the hepatocyte growth factor (HGF) receptor c‐Met. Although ectopic expression of CD82 in nonsmall cell lung carcinoma cells did not affect the tyrosine phosphorylation of c‐Met, these cells showed significant suppression of HGF‐induced lamellipodial protrusion and cell migration. CD82 selectively attenuated c‐Met signaling via the Ras‐Cdc42/Rac and the phosphatidylinositol 3‐kinase/Cdc42/Rac pathways. In contrast, another c‐Met signaling pathway that involves phosphatidylinositol 3‐kinase/Akt and phosphatidylinositol 3‐kinase/mitogen activated protein kinase was not affected by CD82. Signaling adapter proteins for c‐Met, such as Grb2 and p85, exhibited reduced association with c‐Met in cells that ectopically expressed CD82. These results indicate that the CD82‐c‐Met complex inhibits HGF‐induced cancer cell migration by the inactivation of small GTP‐binding proteins of the Rho family via c‐Met adapter proteins. © 2007 Wiley‐Liss, Inc.
KLF6 allelic loss is associated with tumor recurrence and markedly decreased survival in head and neck squamous cell carcinoma
Authors: Teixeira Miriam S. , Camacho‐Vanegas Olga , Fernandez Yolanda , Narla Goutham , DiFeo Analisa , Lee Bryant , Kalir Tamara , Friedman Scott L. , Schlecht Nicolas F. , Genden Eric M. , Urken Mark , Brandwein‐Gensler Margaret , Martignetti John A. 
Published: July 2007

AbstractThe Krüppel‐like transcription factor (KLF6) gene is a tumor suppressor gene (TSG) reported to be dysregulated and inactivated through loss of heterozygosity (LOH) and/or somatic mutation in a number of major human cancers. The aim of the present study was to examine KLF6 gene status and expression in head and neck squamous cell carcinomas (HNSCC). A collection of 81 well‐characterized oral and oropharyngeal HNSCC samples were analyzed for evidence of KLF6 LOH and mutation and differences in expression patterns between normal and cancerous tissues and these findings were correlated with clinicopathological variables. We also tested the effect of KLF6 inhibition in HNSCC cell lines on proliferation and p21 expression. LOH was found in approximately 30% of cases and was strongly correlated with cancer progression, tumor recurrence and decreased patient survival. Overall, median survival of patients with LOH was less than half (19 vs. 41 months, p = 0.036, stratified on stage) than those without loss. Risk of death for patients with LOH was 8 times greater independent of tumor size, nodal status, tobacco smoking or treatment modality (HR 7.89, 95% CI: 1.9–32.4). Subsequent analyses revealed KLF6 mutations in only 2 of 20 samples, but altered subcellular protein localization in 64% of tumors. Targeted stable reduction of KLF6 in HNSCC cell lines increased cellular proliferation while decreasing p21 expression. Taken together, these findings suggest that KLF6 LOH represents a clinically‐relevant biomarker predicting patient survival and tumor recurrence and that dysregulation of KLF6 function plays an important role in HNSCC progression. © 2007 Wiley‐Liss, Inc.
Wear of medical grade silicone rubber against titanium and ultrahigh molecular weight polyethylene
Authors: Pylios T. , Shepherd D. E. T. 
Published: July 2007

AbstractThe replacement of arthritic small joints, such as the fingers and wrist, has typically involved the use of one‐piece silicone rubber implants. Newer designs have involved the silicone moving against either a titanium or ultrahigh molecular weight polyethylene (UHMWPE) component. The aim of this study was to investigate the wear of medical grade silicone rubber against titanium and UHMWPE. A pin‐on‐disc apparatus was used to slide a titanium and UHMWPE pin against a silicone disc, in the presence of either a Ringer's solution or bovine serum lubricant. Testing was undertaken at a sliding speed of 0.079 m/s and was continued for 10 km. Wear factors for titanium against silicone were 40.0 × 10−6 mm3/N m and 66.5 × 10−6 mm3/N m for bovine serum and Ringer's solution, respectively. The wear factors for UHMWPE against silicone were higher with values of 84.4 × 10−6 mm3/N m and 88.3 × 10−6 mm3/N m for bovine serum and Ringer's solution, respectively. The results of this study will be useful in future designs of finger and wrist implants that combine silicone rubber with either titanium or UHMWPE. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008
Sensitized chemiluminescence determination of fluorouracil in pharmaceutical and biological fluids based on potassium permanganate oxidation in the presence of formaldehyde
Authors: Sun Hanwen , Li Liqing , Chen Xueyan 
Published: July 2007

AbstractA novel chemiluminescence (CL) reaction system was established for the anticancer drug fluorouracil (5‐FU). We developed a formaldehyde‐sensitized CL emission mechanism for 5‐FU‐KMnO4‐HCl systems by comparing the fluorescence emission with CL spectra. The illuminant was the singlet‐state bimolecular oxygen 1O21O2(1Δg1Δg), which is from 1O2(1Δg) produced in the reaction system, and emits CL spectra at 639 nm. The presence of formaldehyde can sensitize CL emission by accelerating the generation of 1O2(1Δg). The optimum conditions for CL emission were investigated. The relationship between the relative CL intensity and the concentration of the analyte showed good linearity. The determination limit was 3 × 10−8 g mL−1. The relative standard deviation (SD) was 1.8%. The proposed method was applied to the determination of fluorouracil in pharmaceutical preparations and biological fluids, and satisfactory results were obtained. J. Clin. Lab. Anal. 21:213–219, 2007. © 2007 Wiley‐Liss, Inc.
Comparison of manual and automated nucleic acid extraction from whole‐blood samples
Authors: Riemann Kathrin , Adamzik Michael , Frauenrath Stefan , Egensperger Rupert , Schmid Kurt W. , Brockmeyer Norbert H. , Siffert Winfried 
Published: July 2007

AbstractNucleic acid extraction and purification from whole blood is a routine application in many laboratories. Automation of this procedure promises standardized sample treatment, a low error rate, and avoidance of contamination. The performance of the BioRobot M48 (Qiagen) and the manual QIAmp® DNA Blood Mini Kit (Qiagen) was compared for the extraction of DNA from whole blood. The concentration and purity of the extracted DNAs were determined by spectrophotometry. Analytical sensitivity was assessed by common PCR and genotyping techniques. The quantity and quality of the generated DNAs were slightly higher using the manual extraction method. The results of downstream applications were comparable to each other. Amplification of high‐molecular‐weight PCR fragments, genotyping by restriction digest, and pyrosequencing were successful for all samples. No cross‐contamination could be detected. While automated DNA extraction requires significantly less hands‐on time, it is slightly more expensive than the manual extraction method. J. Clin. Lab. Anal. 21:244–248, 2007. © 2007 Wiley‐Liss, Inc.
Linkage studies for T2D in Chop and C/EBPbeta chromosomal regions in Italians
Authors: Gragnoli Claudia , Pierpaoli Laura , Piumelli Nunzia , Chiaramonte Francesco 
Published: July 2007

AbstractThe genes causing type 2 diabetes (T2D), a complex heterogeneous disorder, differ and/or overlap in various populations. Among others there are two loci in linkage to T2D, the chromosomes 20q12‐13.1 and 12q15. These two regions harbor two genes, C/EBPbeta and CHOP, which are excellent candidate genes for T2D. In fact, C/EBPbeta protein cooperates with HNF4alpha (MODY1, monogenic form of diabetes) and 1alpha (MODY3, monogenic form of diabetes). C/EBPbeta mediates suppression of insulin gene transcription in hyperglycemia and may contribute to insulin‐resistance. It interacts in a complex pathway with the CHOP protein. CHOP may play a role in altered beta‐cell glucose metabolism, in beta‐cell apoptosis, and in lack of beta‐cell replication. Thus, both C/EBPbeta and CHOP genes may independently and interactively contribute to T2D. The chromosomal regions targeting C/EBPbeta and CHOP genes have never been previously explored in T2D. We planned to identify their potential contribution to T2D in Italians. We have genotyped a group of affected siblings/families with both late‐ and early‐onset T2D around the C/EBPbeta and the CHOP genes. We have performed non‐parametric linkage analysis in the total T2D group, in the late‐onset and the early‐onset group, separately. We have identified a suggestive linkage to T2D in the CHOP gene locus in the early‐onset T2D group (P = 0.04). We identified the linkage to T2D in the chromosome 12q15 region in the early‐onset T2D families and specifically target the CHOP gene. Our next step will be the identification of CHOP gene variants, which may contribute to the linkage to T2D in Italians. J. Cell. Physiol. 213: 552–555, 2007. © 2007 Wiley‐Liss, Inc.
Promotion of Fas‐mediated apoptosis in Type II cells by high doses of hepatocyte growth factor bypasses the mitochondrial requirement
Authors: Zhao Yongge , DiFrancesca Daniell , Wang Xue , Zarnegar Reza , Michalopoulos George K. , Yin Xiao‐Ming 
Published: July 2007

AbstractThe death receptor pathway is coupled to the mitochondria apoptosis pathway. However, mitochondrial participation, which is stimulated by Bid but suppressed by Bcl‐2/Bcl‐xL, is required in certain cells (Type II), but not in others (Type I). While these differences were originally characterized in the lymphoid cell lines, the typical Type II cells are represented by hepatocytes in vivo. The molecular mechanisms that distinguish Type II from Type I cells and the regulation are not fully understood. Fas can be sequestered by the HGF receptor c‐Met and high doses of HGF can promote cell death by freeing Fas from c‐Met complex. We thus reasoned that treatment of the Type II cells with high doses of HGF could enhance Fas‐mediated apoptosis and spare the mitochondria amplification. Indeed, such treatment led to increased apoptosis in Type II lymphoid cells, which could not be blocked by Bcl‐xL. Moreover, significant hepatocyte apoptosis was induced by this scheme in the absence of Bid with increased dissociation of Fas from c‐Met. These findings indicate that high doses of HGF could be used to promote apoptosis in Type II cells bypassing the requirement for mitochondria activation. J. Cell. Physiol. 213: 556–563, 2007. © 2007 Wiley‐Liss, Inc.
ATP depletion inhibits the endocytosis of ClC‐2
Authors: Dhani Sonja U. , Kim Chiaw Patrick , Huan Ling‐Jun , Bear Christine E. 
Published: July 2007

AbstractThe chloride channel, ClC‐2 is expressed ubiquitously and participates in multiple physiological processes. In particular, ClC‐2 has been implicated in the regulation of neuronal chloride ion homeostasis and mutations in ClC‐2 are associated with idiopathic generalized epilepsy. Despite the physiological and pathophysiological significance of this channel, its regulation remains incompletely understood. The functional expression of ClC‐2 at the cell surface has been shown to be enhanced by depletion of cellular ATP, implicating its possible role in cellular energy sensing. In the present study, biochemical assays of cell surface expression suggest that this gain of function reflects, in part, an increase in channel number due to the reduction in ClC‐2 internalization by endocytosis. Cell surface expression of the disease‐causing mutant: G715E, thought to lack wild‐type nucleotide binding affinity, is similarly affected, suggesting that ATP‐depletion modifies the function of proteins in the endocytic pathway rather than ClC‐2 directly. Using a combination of immunofluorescence and biochemical studies, we confirmed that ClC‐2 is internalized via dynamin‐dependent endocytosis and that the change in surface expression evoked by ATP depletion is partially mimicked by inhibition of dynamin function using a dynamin dominant‐negative mutant (DynK44A). Furthermore, trafficking via the early endosomal compartment occurs in part through rab5‐associated vesicles and recycling of ClC‐2 to the cell surface occurs through a rab11 dependent pathway. In summary, we have determined that the internalization of ClC‐2 by endocytosis is inhibited by metabolic stress, highlighting the importance for understanding the molecular mechanisms mediating the endosomal trafficking of this channel. J. Cell. Physiol. 214:273–280, 2008. © 2007 Wiley‐Liss, Inc.
The association between the SH2‐containing inositol polyphosphate 5‐Phosphatase 2 (SHIP2) and the adaptor protein APS has an impact on biochemical properties of both partners
Authors: Onnockx Sheela , De Schutter Julie , Blockmans Marianne , Xie Jingwei , Jacobs Christine , Vanderwinden Jean‐Marie , Erneux Christophe , Pirson Isabelle 
Published: July 2007

AbstractSHIP2 (SH2‐containing inositol polyphosphate 5‐phosphatase 2) is a phosphatidylinositol (3,4,5)‐trisphosphate (PtdIns(3,4,5)P3) 5‐phosphatase containing various motifs susceptible to mediate protein‐protein interaction. In cell models, SHIP2 negatively regulates insulin signalling through its catalytic PtdIns(3,4,5)P3 5‐phosphatase activity. We have previously reported that SHIP2 interacts with the c‐Cbl associated protein (CAP) and c‐Cbl, proteins implicated in the insulin cellular response regulating the small G protein TC10. The first steps of the TC10 pathway are the recruitment and tyrosine phosphorylation by the insulin receptor of the adaptor protein with Pleckstrin Homology and Src Homology 2 domains (APS). Herein, we show that SHIP2 can directly interact with APS in 3T3‐L1 adipocytes and in transfected CHO‐IR cells (Chinese hamster ovary cells stably transfected with the insulin receptor). Upon insulin stimulation, APS and SHIP2 are recruited to cell membranes as seen by immunofluorescence studies, which is consistent with their interaction. We also observed that SHIP2 negatively regulates APS insulin‐induced tyrosine phosphorylation and consequently inhibits APS association with c‐Cbl. APS, which specifically interacts with SHIP2, but not PTEN, in turn, increases the PtdIns(3,4,5)P3 5‐phosphatase activity of SHIP2 in an inositol phosphatase assay. Co‐transfection of SHIP2 and APS in CHO‐IR cells further increases the inhibitory effect of SHIP2 on Akt insulin‐induced phosphorylation. Therefore, the interaction between APS and SHIP2 provides to both proteins potential negative regulatory mechanisms to act on the insulin cascade. J. Cell. Physiol. 214:260–272, 2008. © 2007 Wiley‐Liss, Inc.
Impairment of erythroid and megakaryocytic differentiation by a Leukemia‐Associated and t(9;9)‐derived fusion gene product, SET/TAF‐Iβ‐CAN/Nup214
Authors: Saito Shoko , Nouno Kaoru , Shimizu Ritsuko , Yamamoto Masayuki , Nagata Kyosuke 
Published: July 2007

AbstractSET‐CAN associated with the t(9;9) in acute undifferentiated leukemia encodes almost the entire sequence of SET and the C‐terminal two‐third portion of CAN, including the FG repeat region. To clarify a role(s) of SET‐CAN in leukemogenesis, we developed transgenic mice expressing SET‐CAN under the control of the Gata1 gene hematopoietic regulatory domain that is active in distinct sets of hematopoietic cells. SET‐CAN transgenic mice showed anemia, thrombocytopenia, and splenomegaly. A significant number of transgenic mice started dying after 6 months post‐birth, being in good agreement with the fact that red blood cells and platelets decreased. We found that a significant number of c‐kit+ myeloid cells appeared in peripheral blood in transgenic mice. Characterization of the bone marrow cells of transgenic mice indicated impairment in hematopoietic differentiation of erythroid, megakaryocytic, and B cell lineages by SET‐CAN. Transgenic mice, in particular, exhibited a high population of the c‐kit+Sca‐1+Lin− fraction in bone marrow cells compared with that of the control littermates. Our results demonstrate that SET‐CAN blocks the hematopoietic differentiation program—one of the characteristics of acute myeloid leukemia. J. Cell. Physiol. 214: 322–333, 2008. © 2007 Wiley‐Liss, Inc.
Neurotropic effects of venoms and other factors that promote prey acquisition
Authors: Gennaro Joseph Francis , Hall Hollis Pace , Casey Ernest Raymond , Hayes William Kent 
Published: July 2007

AbstractMammals envenomed by either the Eastern diamondback rattlesnake (Crotalus adamanteus) or the cottonmouth (Agkistrodon piscivorus piscivorus) exhibit an immediate but transitory pupillar contraction, a parasympathomimetic effect mediated through the ciliary ganglion that can be prevented by a retrobulbar injection of anesthetic. The venom of the cottonmouth injected into the lymph spaces of the frog (Rana pipiens) produces an immediate and total collapse of the lung sacs. Applied locally to the surface, it produces a constriction that eventually collapses the entire sac. Tests of venoms and toxins from both anterior and posterior parts of the venom apparatus indicate that the lung‐collapsing moiety originates in the accessory, not the main portion of the venom gland. This is the first example of a functional specialization within the whole structure. It seems that this factor is elaborated primarily in snakes that prey upon frogs, although insufficient data are available from this study to confirm this. In both reptile species, the predatory strike is accompanied by an immediate effect, perhaps mediated by the parasympathetic nervous system, designed to incapacitate the prey and facilitate capture. These effects cannot now be attributed to neurotoxins because the effect of the former is transitory (and not lethal) and neither has been purified sufficiently to determine potency or structure. Both take part in securing, but not killing, the prey, and both directly oppose the sympathetic nervous system “fright‐fight/flight” response. Evidence is presented to support the possibility that known epigenetic mechanisms are capable of effecting heritable changes in gene expression that could allow for the development of factors that facilitate prey acquisition and promote rapid adaptation to environmental change. J. Exp. Zool. 307A:488–499, 2007. © 2007 Wiley‐Liss, Inc.