bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting axillary residual disease after neoadjuvant therapy in breast cancer using baseline MRI and ultrasound
Authors: Malhaire Caroline , Umay Ozgun , Cockenpot Vincent , Selhane Fatine , Ramtohul Toulsie , Reyal Fabien , Pierga Jean-Yves , Romano Emanuella , Vincent-Salomon Anne , Kirova Youlia , Laas Enora , Brisse Hervé J. , Frouin Frédérique 
Published: February 2025

Abstract

Objectives
To predict axillary node residual disease in women treated for node-positive breast cancer (BC) by neoadjuvant therapy (NAT), using breast BI-RADS MRI features and axillary ultrasound at baseline.

Material and methods
In this single-center, retrospective study, women with node-positive BC who underwent NAT between 2016 and 2021 were included. Pre-treatment axillary US and breast MRIs were evaluated using the BI-RADS lexicon and T2 features, including Breast Edema Score. Univariate and multivariate logistic regression analyses were conducted for the prediction of axillary residual disease (ARD). A multivariable model based on logistic regression was trained and evaluated on randomly split train and test sets (7:3 ratio).

Results
Out of the 141 women, 41% had post-NAT ARD. Axillary metastasis was independently associated with luminal subtype (odds ratio (OR), 25.5; p < 0.001), anterior tumor location (OR, 14.1; p = 0.008), and cortical thickening ≥ 7 mm (OR, 6.09; p = 0.002). Intratumoral T2 high signal intensity was protective (OR, 0.16; p = 0.006), while Ki67 had a marginal association (p = 0.064). In the training and test sets, the model, which is available online, achieved AUCs of 0.860 (95% CI: 0.783–0.936) and 0.843 (95% CI: 0.714–0.971), respectively. Anterior depth location and cortical thickening greater than 7 mm were also independently associated with post-NAT axillary burden.

Conclusion
Adjusting for BC subtype and KI-67 index, the anterior third location of BC, a cortical thickness greater than 7 mm, and the absence of intratumoral T2 hyperintensity is predictive of ARD after NAT.

Key Points

Question

What baseline imaging-based predictive models can identify patients at risk of persistent nodal disease after neoadjuvant therapy?

Findings

Baseline US cortical thickness superior to 7 mm, anterior tumor location, and absence of an intratumoral high signal on T2-weighted MRI predict residual axillary disease.

Clinical relevance

Our predictive model, available online at:
litoic.shinyapps.io/LNPred_Apps, including breast cancer subtype, Ki-67 index level, breast cancer location, intratumoral signal intensity on T2WI, and initial lymph node thickness, could guide post-NAT axillary management.

Graphical Abstract
Perivascular glial reactivity is a feature of phosphorylated tau lesions in chronic traumatic encephalopathy
Authors: Osterman Chelsie , Hamlin Danica , Suter Catherine M. , Affleck Andrew J. , Gloss Brian S. , Turner Clinton P. , Faull Richard L. M. , Stein Thor D. , McKee Ann , Buckland Michael E. , Curtis Maurice A. , Murray Helen C. 
Published: February 2025

Abstract
Chronic traumatic encephalopathy (CTE), a neurodegenerative disease associated with repetitive head injuries, is characterised by perivascular hyperphosphorylated tau (p-tau) accumulations within the depths of cortical sulci. Although the majority of CTE literature focuses on p-tau pathology, other pathological features such as glial reactivity, vascular damage, and axonal damage are relatively unexplored. In this study, we aimed to characterise these other pathological features, specifically in CTE p-tau lesion areas, to better understand the microenvironment surrounding the lesion. We utilised multiplex immunohistochemistry to investigate the distribution of 32 different markers of cytoarchitecture and pathology that are relevant to both traumatic brain injury and neurodegeneration. We qualitatively assessed the multiplex images and measured the percentage area of labelling for each marker in the lesion and non-lesion areas of CTE cases. We identified perivascular glial reactivity as a prominent feature of CTE p-tau lesions, largely driven by increases in astrocyte reactivity compared to non-lesion areas. Furthermore, we identified astrocytes labelled for both NAD(P)H quinone dehydrogenase 1 (NQO1) and L-ferritin, indicating that lesion-associated glial reactivity may be a compensatory response to iron-induced oxidative stress. Our findings demonstrate that perivascular inflammation is a consistent feature of the CTE pathognomonic lesion and may contribute to the pathogenesis of brain injury-related neurodegeneration.
Transcutaneous electrical acustimulation promotes wound healing in mice by modulating signaling molecules and mitochondria function
Authors: Han Rong , Chen Menghua , Peng Wang , Yue Jianbo , Hu Jinlian 
Published: February 2025

Abstract
Previous research has identified a variety of factors that contribute to the development and maintenance of wounds. Concurrently, electroacupuncture has been demonstrated to facilitate wound healing. However, the effects of transcutaneous electrical acustimulation (TEA) on wound healing, as well as its relationship with key factors such as Wnt3a, TGF-β, Akt, c-Myc, VEGF-A, SP1, nitric oxide (NO), and mitochondrial function, remain largely unexplored. We hypothesize that TEA will activate the signaling factors and enhance mitochondrial functions to promote the repair of skin wounds in mice. An in vivo experimental study was conducted utilizing mouse models with skin wounds. The study comprised three groups: a TEA treatment with wound group, a skin wound model group, and a control group. Wound areas were measured by calculating the product of the length and width of each wound using calipers. Single-cell suspensions were prepared by excising the wound and the immediately surrounding tissue. These suspensions were stained with Trypan blue to assess cell viability, with specific probes to measure the rate of reactive oxygen species (ROS) positivity, and with reagents to quantify NO content. Western blotting (WB) was employed to evaluate protein levels associated with tissue changes, while quantitative polymerase chain reaction (qPCR) was used to assess RNA expression levels. Immunofluorescence staining was performed to visualize protein content and other relevant cellular structures within tissue sections. TEA exhibited anti-inflammatory properties and promoted wound healing in mice. Western blot analysis revealed that TEA enhanced the expression of proteins associated with Wnt3a, TGF-β, Akt, c-Myc, VEGF-A, and SP1 during the wound healing process. Immunofluorescence staining of tissue sections indicated that TEA upregulated the expression of COL1A1, MFN1, GRP75, GRP78, GRP75/ROS, GRP78/ROS, ISCU, and UCP1 while downregulating FIS1. Additionally, qPCR results demonstrated that TEA promoted the expression of IL-10 and miRNA205-5p while inhibiting MMP9 levels. TEA modulates various signaling molecules, influences chaperone proteins related to stress recovery responses, along with mitochondrial dynamics and metabolism.

Graphical abstract
Developmental processes in the Rosaceae through the lens of DNA and RNA methylation
Authors: Lawson Tamunonengiye-Ofori , Selva Juan-Pablo , Carballo José , Caccamo Mario , Sargent Daniel J. , Šurbanovski Nada 
Published: February 2025

Abstract

Main conclusion
This review discusses the DNA and RNA methylation pathways and their biological roles in Rosaceae developmental processes relevant for breeding and production.

Abstract
The Rosaceae is a plant family of great importance for human nutrition and health. Many traits and developmental processes of the Rosaceae are influenced by epigenetic methylation, functions of which are now being unravelled in several important species of this family. Methylation of DNA at the 5th position of cytosine (5mC) is a well-established epigenetic mark that affects important cellular processes such as gene expression and genome stability and is involved in a wide range of plant biological functions. Further to this, recent technological advances have uncovered other naturally occurring chemical modifications of DNA and RNA as additional layers of regulatory epigenetic information in plants. In this review we give a comprehensive summary of plant 5-methylcytosine DNA methylation mechanisms and review their components identified in species of the Rosaceae family. We detail and discuss the role of 5mC DNA methylation dynamics in Rosaceae developmental processes, including phase transition, bud development, bud dormancy, plant architecture, plant regeneration, fruit development, ripening and senescence. We then review recent advances in understanding the newly identified nucleic acid modifications, N

6
-adenosine methylation of DNA (6mA) and RNA (m6A) as additional epigenetic mechanisms. We summarise identified components of adenosine methylation pathways in the Rosaceae and discuss the emerging roles of this modification in plant development including recent findings in Rosaceous species. Integrating epigenetic aspects of plant development with plant genetics and physiology is crucial for understanding biological processes in Rosaceous plants.
On the maximization of the likelihood for the generalized gamma distribution: the modified maximum likelihood approach
Authors: Arslan Talha , Acitas Sukru , Senoglu Birdal 
Published: February 2025

Abstract
Maximum likelihood (ML) estimation of parameters of the generalized gamma (GG) distribution has been considered in several papers, and some of them stated that the ML estimation has some computational difficulties. Therefore, different approaches including numerical methods have been proposed for the ML estimation of parameters of the GG distribution. However, it is known that using numerical methods may have some drawbacks, e.g., non-convergence of iterations, multiple roots, and convergence to the wrong root. In this study, we rehabilitate the ML procedure via the modified ML (MML) methodology and obtain the likelihood equations in which two of them have explicit solutions, and the remaining one should be solved numerically. Since the MML methodology explicitly solves two of three likelihood equations, the mentioned drawbacks are alleviated. We also propose a simple algorithm to obtain the estimates of the parameters of the GG distribution. Then, the GG distribution is used for modeling the real data sets, and the performance of the proposed algorithm is compared with the Broyden–Fletcher–Goldfarby–Shanno (BFGS) and Nelder–Mead (NM) algorithms. The results show that the proposed algorithm is preferable to the BFGS and NM algorithms in terms of computational sense when considering the GG distribution.
Screening for depression, anxiety, and psychological distress associated with low back pain by musculoskeletal and spinal clinicians. A scoping review
Authors: Sugrue Julie , McKenna Sean , Purtill Helen , O’Sullivan Kieran 
Published: February 2025

Abstract

Purpose
A biopsychosocial assessment of a person with low back pain (LBP) should include the detection of psychological risk factors. Pragmatically, clinicians often use their clinical impression rather than questionnaires to screen for psychological risk factors. This scoping review explores how accurately depression, anxiety, and psychological distress can be identified by musculoskeletal (MSK) or spinal clinicians using clinical impression alone.

Methods
The study protocol was pre-registered on Open Science Framework (OSF) https://osf.io/pt3a6/. A comprehensive search strategy using five databases (MEDLINE, CINAHL, Academic Search Complete, APA PsycInfo, APA PsycArticles) was conducted by two independent reviewers, with additional searching of references. Studies must have included adults with LBP, who were assessed by MSK or spinal clinician for depression, anxiety or psychological distress using their clinical impression as well as a reference standard questionnaire. Data were synthesised descriptively and thematically for each psychological factor separately.

Results
Eight studies involving MSK clinicians (n = 4) and spinal clinicians (n = 4) were eligible. Four different reference standard questionnaires were used. Overall MSK and spinal clinicians were not accurate at identifying depression, anxiety or psychological distress in adults with LBP using only clinical impression. Mean sensitivity for accurate identification of high reference standard questionnaire scores for depression, anxiety, and psychological distress ranged between 12.0% and 40.0%. Mean sensitivity was marginally higher when the threshold was reduced to analyse clinician ability to accurately capture any abnormal psychological state (34.0% to 54.1%). Mean specificity values across higher thresholds ranged from 88.7% to 93.6%.

Conclusion
Overall, clinician impression alone is insufficient for MSK and spinal clinicians to accurately identify depression, anxiety and psychological distress in adults with LBP. The general tendency of the clinicians was to underestimate their presence. Without formal assessment of the psychological status of patients with LBP, clinicians will have an incomplete understanding of concordant psychological factors, and may fail to notice the requirement to refer on for appropriate psychological management.
Clinical and epidemiological differences in staphylococcal osteoarticular infections: insights for developing hospital-based infection control interventions
Authors: Figueiredo João , Lindo Jorge , Chaves Catarina , Nogueira Célia 
Published: February 2025

Abstract

Purpose
Osteoarticular infections (OAI) are serious clinical conditions with Staphylococcus aureus and Coagulase-negative Staphylococcus (CoNS) responsible for up to two-thirds of cases. This work aimed to compare the epidemiological, clinical, and microbiological characteristics of OAI caused by S. aureus versus CoNS to aid in clinical management and infection control strategies.

Methods
A single-centre retrospective study was performed at the Centro Hospitalar e Universitário de Coimbra for the period of January 2011 to December 2021. A total of 458 cases of OAI were gathered. Data was retrieved from medical records and statistical analysis was performed with SPSS.

Results

S. aureus accounted for 60.7% of infections, followed by S. epidermidis (29.9%). Independent risk factors for S. aureus infections included being male (p < 0.001; OR = 0.47) and a history of osteomyelitis (p < 0.001; OR = 0.18). In contrast, CoNS infections were associated with older age (p = 0.018), carrying a prosthetic device (p < 0.001; OR = 2.92), and a prior periprosthetic infection (p = 0.023; OR = 1.86). Both groups exhibited significant antimicrobial resistance, with CoNS showing greater resistance to gentamicin, linezolid, teicoplanin and trimethoprim-sulfamethoxazole, while S. aureus was more commonly resistant to clindamycin.

Conclusion
Our findings show the distinct characteristics of OAI caused by S. aureus and CoNS, highlighting the need for targeted risk factor management and tailored empiric antibiotic therapy to reduce incidence and improve outcomes.
Three-dimensional architecture of the palmar plate of the thumb metacarpophalangeal joint in infant macaque
Authors: Sato Hiroko , Shimada Tatsuo , Hosoyama Tsuguaki , Shibuta Yutaro , Kaku Nobuhiro 
Published: February 2025

Abstract
The palmar plate is a crucial structural part of hand, associated with metacarpophalangeal and interphalangeal joints. Pediatric disorders involving the palmar plate of thumb metacarpophalangeal joint include trigger thumb, hyperextension, instability, and dislocation. While anatomical differences exist between children and adults, detailed microstructure evaluations in infants remain unexplored. In this study, we provide a histological and structural assessment of the previously unresolved microstructure of the palmar plate in the thumb metacarpophalangeal joint of infant Japanese macaques (Cercopithecidae, Macaca fuscata), a relevant model for human development. Histological staining (light microscopy) and scanning electron microscopy were employed to visualize the three-dimensional microstructure. The palmar plate of the infant macaque was found to contain (1) elastic fibers, (2) hyaline cartilage composed of type II collagen, and (3) type I collagen fibers arranged in distinct patterns. The cartilaginous region exhibited a reticulate fiber arrangement on its periphery, while the membranous region displayed dense and complex fibers on the proximal phalanx side and parallel on the metacarpal side, respectively. This is the first comprehensive three-dimensional investigation of the infant’s thumb’s palmar microanatomy, providing a foundation for understanding its development and implications for pediatric disorders.
Enhancing display resolution through virtual pixel creation: a software-based approach for existing displays
Authors: Carmona-Ballester David , Bonaque-González Sergio , Trujillo-Sevilla Juan M. , Ceruso Sabato , Rodríguez-Ramos José M. 
Published: February 2025

Abstract
The increasing demand for high-resolution displays has spurred advancements in pixel density; however, certain applications, such as head-mounted displays (HMDs) and image projectors, still suffer from noticeable pixel structures that degrade user experience. Conventional methods of resolution enhancement are often complex and costly, limiting their practicality. To address this, we propose a software-based methodology for improving screen resolution through controlled optical defocus. This method introduces a blur into the system, generating overlapping virtual pixels in the image space. The original image is pre-compensated, considering the altered intensity distribution caused by these virtual pixels. Consequently, the displayed image is perceived as having higher resolution than the native display. We present the mathematical foundations of this methodology, which were validated through computational analysis and real-world implementations on HMDs and projectors. The proposed approach distinguishes itself from previous works on overlapping sub-frames by requiring only a single display and a single optical element, offering a simpler and more cost-effective solution. Experimental results demonstrate a significant reduction in the screen-door effect for HMDs and an enhanced resolution for projectors, with minimal loss of quality, particularly in contrast. The findings highlight the potential of our approach to open new avenues for the development of these technologies. By providing a practical and effective means of resolution improvement, our methodology holds promise for addressing the challenges associated with pixel structures in various display applications.
The relative risk of infection in people with multiple sclerosis using disease-modifying treatment: a systematic review of observational studies
Authors: Leung M. W. Y. , Garde E. M. W. Van de , Uitdehaag B. M. J. , Klungel O. H. , Bazelier M. T. 
Published: February 2025

Abstract

Background
Some disease-modifying treatments (DMTs) for multiple sclerosis (MS) increase the risk of infection, but it remains unknown how the risk compares between trials and observational studies.

Objective
To assess the current state of observational research on the risk of infection in people with MS and using DMTs.

Methods
PubMed and Embase were searched for observational studies published on or before 4 April 2023 describing infection in people with MS, with a comparison of at least 1 DMT to no DMT or another DMT. We examined which DMT contrasts and types of infection were studied and how often; and compared observational results of the most frequently studied DMT to trial data from a network meta-analysis.

Results
Out of 5373 search records 22 papers were eligible, of which 5 reported relative risks (RRs). In total, 9 DMTs were studied. Out of 45 possible contrasts, 9 were not studied, and 19 once. The most assessed specific type of infection was neurological (n = 11/22 studies). Natalizumab was the most studied DMT contrasting 7 other DMTs or no DMT, with 12 RRs reported. Point estimates of the RRs (compared to no DMT) for respiratory and urinary tract infections were in opposite direction compared to trial data.

Conclusion
Observational study data on the risk of infection in people with MS on DMT are sparse. The growing availability of real-world data on MS and DMT use provides an opportunity to study specific infections on DMT, which is particularly valuable to populations underrepresented in trials.
Effect of Er: YAG laser and different surface treatment methods on the push-out bond strength of glass fiber post to self adhesive resin cement
Authors: Elalfy Nouran Samy Mohammed , Elbasty Reham Said 
Published: February 2025

Abstract
To compare the push-out bond strength of adhesive resin cement and glass fiber posts (GFP) at different root levels after exposure to Er: YAG laser irradiation compared to other conventional surface treatment procedures. A total of 24 mandibular premolars were decoronated, root canal treatment was done, post spaces were prepared, and roots were mounted in acrylic resin blocks. Fiber posts were divided into four groups (n = 6) according to surface treatment methods: (1) silane only (control group), (2) Er: YAG laser 1.5 W + silane, (3) 30% hydrogen peroxide + silane, (4) sandblasting with 50 μm aluminum oxide particles + silane. GFP were cemented using self-adhesive resin cement. Scanning electron microscope images with 500x magnification were taken for all groups. Push-out test was performed using a universal testing machine at different root levels. The difference between groups was statistically significant with laser group recording the highest mean ± SD value of push-out bond strength (5.668042 ± 1.16 MPa), followed by the H2O2 group, then the control group, meanwhile the lowest value was recorded with Sand-blasting group. There were no statistically significant differences between the Control group and Er: YAG group; Control group and sandblasted group. The difference between the radicular regions was not statistically significant, with the middle region recorded the highest push-out bond strength (4.746851 ± 0.73 MPa). GPF surface treatment using an Er: YAG laser is effective as it increases the retention to resin cement, while sandblasting decreases fiber post retention to resin cement. The hydrogen peroxide and the control groups give similar bond strength. The middle and apical regions of GFP have better retention to resin cement than the coronal one.