bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRIP-Br2 promotes oncogenesis in nude mice and is frequently overexpressed in multiple human tumors
Authors: Cheong Jit Kong , Gunaratnam Lakshman , Zang Zhi Jiang , Yang Christopher M , Sun Xiaoming , Nasr Susan L , Sim Khe Guan , Peh Bee Keow , Rashid Suhaimi Bin Abdul , Bonventre Joseph V , Salto-Tellez Manuel , Hsu Stephen I 
Published: January 2009

Abstract

Background
Members of the TRIP-Br/SERTAD family of mammalian transcriptional coregulators have recently been implicated in E2F-mediated cell cycle progression and tumorigenesis. We, herein, focus on the detailed functional characterization of the least understood member of the TRIP-Br/SERTAD protein family, TRIP-Br2 (SERTAD2).

Methods
Oncogenic potential of TRIP-Br2 was demonstrated by (1) inoculation of NIH3T3 fibroblasts, which were engineered to stably overexpress ectopic TRIP-Br2, into athymic nude mice for tumor induction and (2) comprehensive immunohistochemical high-throughput screening of TRIP-Br2 protein expression in multiple human tumor cell lines and human tumor tissue microarrays (TMAs). Clinicopathologic analysis was conducted to assess the potential of TRIP-Br2 as a novel prognostic marker of human cancer. RNA interference of TRIP-Br2 expression in HCT-116 colorectal carcinoma cells was performed to determine the potential of TRIP-Br2 as a novel chemotherapeutic drug target.

Results
Overexpression of TRIP-Br2 is sufficient to transform murine fibroblasts and promotes tumorigenesis in nude mice. The transformed phenotype is characterized by deregulation of the E2F/DP-transcriptional pathway through upregulation of the key E2F-responsive genes CYCLIN E, CYCLIN A2, CDC6 and DHFR. TRIP-Br2 is frequently overexpressed in both cancer cell lines and multiple human tumors. Clinicopathologic correlation indicates that overexpression of TRIP-Br2 in hepatocellular carcinoma is associated with a worse clinical outcome by Kaplan-Meier survival analysis. Small interfering RNA-mediated (siRNA) knockdown of TRIP-Br2 was sufficient to inhibit cell-autonomous growth of HCT-116 cells in vitro.

Conclusion
This study identifies TRIP-Br2 as a bona-fide protooncogene and supports the potential for TRIP-Br2 as a novel prognostic marker and a chemotherapeutic drug target in human cancer.
PreS1 epitope recognition in newborns after vaccination with the third-generation Sci-B-Vac™ vaccine and their relation to the antibody response to hepatitis B surface antigen
Authors: Hellström Ulla B , Madalinski Kazimierz , Sylvan Staffan PE 
Published: January 2009

AbstractBackgroundSci-B-Vac™ is a recombinant, hepatitis B vaccine derived from a mammalian cell line and containing hepatitis B surface antigen (HBsAg) as well as preS1 and preS2 antigens. Few studies have been performed on the antibody responses to preS1 in relation to the antibody to hepatitis B surface antigen (anti-HBs) response during immunisation of healthy children with preS-containing vaccines.ResultsIn this study 28 healthy newborns were randomly selected to receive either 2.5 ug or 5.0 ug of the Sci-B-Vac vaccine. Children received three doses of vaccine according to a 0-, 1-, 6-month scheme. Antibodies against the S-protein and three synthetic peptides mimicking three B-cell preS1 epitopes, (21–32 amino acid epitope), (32–47 amino acid epitope) and the C-terminal (amino acid epitope 94–117) were determined at 6 and 9 months. Fourteen (50%) of the 28 newborns had detectable levels of anti-preS1 (21–32) antibodies; 15 (54%) were anti-preS1 (32–47) reactive and 12 (43%) were anti-preS1 (94–117) reactive at 6 or 9 months after initiation of the vaccination. Significantly higher levels of anti-HBs were observed in the sera of patients with detectable anti-preS1 (32–47) reactivity (24 550 ± 7375 IU/L, mean ± SEM) as compared with the non-reactive sera (5991 ± 1530 IU/L, p < 0.05). The anti-HBs levels were significantly lower if none (p < 0.05) or one (p < 0.025) of the preS1 (21–32, 32–47, 94–117) peptides were recognised compared with the anti-HBs levels if two or three peptides were recognised.ConclusionRecognition of several preS1 epitopes, and in particular, the epitope contained within the second half of the hepatocyte binding site localised in the hepatitis B surface protein of the third-generation hepatitis B vaccine is accompanied by a more pronounced antibody response to the S-gene-derived protein in healthy newborns.
Value of ultrasound-guided irrigation and drainage of refractory pyocysts in ADPKD
Authors: Saedi Daryoush , Varedi Peyman , Varedi Payam , Mahmoodi Simin , Gashti Hossein Nejad , Darabi Mohsen 
Published: January 2009

Abstract

Background
Cyst infections is not common in the patients with autosomal dominant polycystic kidney disease (ADPKD) however it may pose major problems to the clinicians because the diagnosis is hampered by lack of reliable imaging techniques for identification of the infected cysts and treatment may be difficult due to poor penetration of antibiotics into the cysts.

Case presentation
We present a case of ADPKD and intractable pyocysts that did not respond to standard antibiotic therapy but successfully treated by using ultrasound-guided cyst puncture, and repeated irrigation and drainage.

Conclusion
Where the experienced interventional radiologists are available, this method can rescue these patients from nephrectomy.
Rupture and intra-peritoneal bleeding of a hepatocellular carcinoma after a transarterial chemoembolization procedure: a case report
Authors: Reso Artan , Ball Chad G , Sutherland Francis R , Bathe Oliver , Dixon Elijah 
Published: January 2009

Abstract

Background
Transarterial chemoembolization (TACE) is a well accepted treatment for inoperable hepatocellular carcinoma (HCC). While minor complications involve 10% of all patients, severe complications are rare.

Case Presentation
We describe a case of a 90-year-old male with a large, superficial HCC who underwent TACE. He had a significant intraperitoneal bleed secondary to tumor rupture immediately following the procedure.

Conclusion
Tumor size and superficial location must be considered risk factors for tumor rupture and related complications.
Review of knee arthroscopy performed under local anesthesia
Authors: Law Billy Kan-Yip , Yung Patrick Shu-Hang , Ho Eric Po-Yan , Chang Joseph Jeremy Hsi-Tse , Kwok Grace Ken , Fung Kwai-Yau , Chan Kai-Ming 
Published: January 2009

Abstract
Local anesthesia for knee arthroscopy is a well documented procedure with diagnostic and therapeutic role. Numerous therapeutic procedures including partial menisectomy, meniscus repair, abrasion chondroplasy, synovectomy, loose body removal can be performed safely and comfortably. Appropriate case selection, anesthetic strategy and technical expertise are the key to smooth and successful surgery.
Vastus medialis cross-sectional area is positively associated with patella cartilage and bone volumes in a pain-free community-based population
Authors: Berry Patricia A , Teichtahl Andrew J , Galevska-Dimitrovska Ana , Hanna Fahad S , Wluka Anita E , Wang Yuanyuan , Urquhart Donna M , English Dallas R , Giles Graham G , Cicuttini Flavia M 
Published: January 2009

Abstract
Introduction
Although vastus medialis and lateralis are important determinants of patellofemoral joint function, their relationship with patellofemoral joint structure is unknown. The aim of this study was to examine potential determinants of vastus medialis and lateralis cross-sectional areas and the relationship between the cross-sectional area and patella cartilage and bone volumes.

Methods
Two hundred ninety-seven healthy adult subjects had magnetic resonance imaging of their dominant knee. Vastus medialis and lateralis cross-sectional areas were measured 37.5 mm superior to the quadriceps tendon insertion at the proximal pole of the patella. Patella cartilage and bone volumes were measured from these images. Demographic data and participation in vigorous physical activity were assessed by questionnaire.

Results
The determinants of increased vastus medialis and lateralis cross-sectional areas were older age (P ≤ 0.002), male gender (P < 0.001), and greater body mass index (P ≤ 0.07). Participation in vigorous physical activity was positively associated with vastus medialis cross-sectional area (regression coefficient [beta] 90.0; 95% confidence interval [CI] 38.2, 141.7) (P < 0.001) but not with vastus lateralis cross-sectional area (beta 10.1; 95% CI -18.1, 38.3) (P = 0.48). The cross-sectional area of vastus medialis only was positively associated with patella cartilage volume (beta 0.6; 95% CI 0.23, 0.94) (P = 0.001) and bone volume (beta 3.0; 95% CI 1.40, 4.68) (P < 0.001) after adjustment for potential confounders.

Conclusions
Our results in a pain-free community-based population suggest that increased cross-sectional area of vastus medialis, which is associated with vigorous physical activity, and increased patella cartilage and bone volumes may benefit patellofemoral joint health and reduce the long-term risk of patellofemoral pathology.
Cerebral microdialysis for detection of bacterial meningitis in aneurysmal subarachnoid hemorrhage patients: a cohort study
Authors: Schlenk Florian , Frieler Katja , Nagel Alexandra , Vajkoczy Peter , Sarrafzadeh Asita S 
Published: January 2009

Abstract
Introduction
Bacterial meningitis (BM) is a severe complication in patients with aneurysmal subarachnoid haemorrhage (SAH). Clinical signs of meningitis are often masked by SAH-related symptoms, and routine cerebrospinal fluid (CSF) analysis fails to indicate BM. Microdialysis (MD) is a technique for monitoring cerebral metabolism in patients with SAH. A cohort study was performed to investigate the value of MD for the diagnosis of BM.

Methods
Retrospectively, 167 patients with SAH in an ongoing investigation on cerebral metabolism monitored by MD were analysed for the presence of BM and related MD changes. Diagnosis of BM was based on microbiological CSF culture or clinical symptoms responding to antibiotic treatment, combined with an increased CSF cell count and/or fever. Levels of MD parameters before and after diagnosis of BM were analysed and compared with the spontaneous course in controls.

Results
BM developed in 20 patients, of which 12 underwent MD monitoring at the time of diagnosis. A control group was formed using 147 patients with SAH not developing meningitis. On the day BM was diagnosed, cerebral glucose was lower compared with the value three days before (p = 0.012), and the extent of decrease was significantly higher than in controls (p = 0.044). A decrease in cerebral glucose by 1 mmol/L combined with the presence of fever ≥ 38°C indicated BM with a sensitivity of 69% and a specificity of 80%. CSF chemistry failed to indicate BM, but the cell count increased during the days before diagnosis (p < 0.05).

Conclusions
A decrease in MD glucose combined with the presence of fever detected BM with acceptable sensitivity and specificity, while CSF chemistry failed to indicate BM. In patients with SAH where CSF cell count is not available or helpful, MD might serve as an adjunct criterion for early diagnosis of BM.
Surgical and medical emergencies on board European aircraft: a retrospective study of 10189 cases
Authors: Sand Michael , Bechara Falk-Georges , Sand Daniel , Mann Benno 
Published: January 2009

Abstract
Introduction
In-flight medical and surgical emergencies (IMEs) onboard commercial aircrafts occur quite commonly. However, little epidemiological research exists concerning these incidents.

Methods
Thirty-two European airlines were asked to provide anonymous data on medical flight reports of IMEs for the years 2002 to 2007. The total number of incidents was correlated to revenue passenger kilometers (rpk). Additionally, on-board births and deaths, flight diversions, flight routes (continental/intercontinental) and involvement of a physician or medical professional in providing therapy were analysed.

Results
Only four airlines, of which two participated in this study, were able to provide the necessary data. A total of 10,189 cases of IMEs were analysed. Syncope was the most common medical condition reported (5307 cases, 53.5%) followed by gastrointestinal disorders (926 cases, 8.9%) and cardiac conditions (509 cases, 4.9%). The most common surgical conditions were thrombosis (47 cases, 0.5%) and appendicitis (27 cases, 0.25%). In 2.8% of all IMEs, an aircraft diversion was performed. In 86% of cases, a physician or medical professional was involved in providing therapy. A mean (standard deviation) of 14 (+/- 2.3, 10.8 to 16.6 interquartile range) IMEs per billion rpk was calculated.

Conclusions
The study demonstrates that although aviation is regulated by a variety of national and international laws, standardised documentation of IMEs is inadequate and needs further development.
Surgery in patients with vascular malformations of the head and neck: value of coagulation disorders
Authors: Wiegand S , Eivazi B , Karger R , Al Kadah B , Sesterhenn A M , Werner J A 
Published: January 2009

Objectives The surgical treatment of congenital vascular malformations always bears the risk of severe bleeding. The aim of this study was to investigate the prevalence of haemostatic abnormalities in patients with congenital vascular malformations of the head and neck which could additionally complicate the complex surgery of vascular malformations. Methods Thirteen patients with congenital vascular malformations of the head and neck, who underwent scheduled surgery, were reviewed regarding their haemostatic tests, including prothrombin time, activated partial thromboplastin time, fibrinogen level, platelet count, platelet function analyzer-epinephrine (PFA-Epi) and -adenosine diphosphate (ADP) (PFA-100®), von Willebrand factor (vWF) and D-dimers. Results In six of 13 patients an impaired primary haemostasis was detected by prolonged PFA-Epi and one of these patients also had a slightly prolonged PFA-ADP. Additionally, two of the patients showed mild coagulation factor deficiency. One patient had a mild vWF deficiency that was not detected by PFA-100®. Conclusion Prior to surgery of congenital vascular malformations of the head and neck, a detailed coagulation analysis is needed in order to identify those patients at increased risk of bleeding. This proceeding is supported by the fact that a relevant portion of the patients with congenital vascular malformations of the head and neck show an impaired primary haemostasis.
First results with a new 1470-nm diode laser for endovenous ablation of incompetent saphenous veins
Authors: Pannier F , Rabe E , Maurins U 
Published: January 2009

Introduction Most of the published EVLA data concern 810, 940, 980 nm diode lasers and 1064 or 1320 nm Nd:Yag laser systems. Major side effects are postoperative pain and bruising. The aim of this study was to show the outcome one year after EVLA of incompetent saphenous veins with a 1470 nm Diode laser (Ceralas E, biolitec). Patients and method Between December 2006 and February 2007, 134 saphenous veins (108 GSV, 26 SSV) in 117 legs of 100 consecutive patients where treated by EVLA for GSV and SSV incompetence. All patients were examined clinically and with duplex by an experienced phlebologist prior to intervention, and at the follow-up visits for complications, occlusion, flow and reflux in the treated vein segment. The clinical evaluation included clinical CEAP and the presence of recurrent varicose veins. Patient satisfaction was assessed by a 0 to 4 scale. Results After a mean follow-up period of 184 days (SD 27) 127 treated veins (102 GSV, 25 SSV) of 111 limbs in 94 patients and after 329 days (SD 14) 105 treated veins (94 GSV, 21 SSV) of 105 limbs in 83 patients were reinvestigated. Six patients were lost to follow up after six months and an additional 11 patients after one year. Up to one year follow-up all treated veins remained occluded. At six months, one new insufficient anterior accessory saphenous vein (AASV) and after 12 months, three new insufficient AASV occurred. After one year 45 patients were very satisfied with the method, 34 were satisfied, three were fairly and one was not satisfied. The mean of all answers was 0.5 (SD 0.5). In three cases phlebitic reactions after 10 days, but no severe complications such as deep vein thrombosis occured. After six months in 9.5% of the legs paresthesia was present in the treated area which reduced to 7.6% after one year. Intake of painkillers was mean 6.7 tablets (SD 3.5). When we compared GSV legs treated with LEED below or above 100 J/cm, the paresthesia rate was significantly lower in the first group with 2.3% compared to 15.5 % in the higher LEED group. The differences for number of days with analgesic intake and for the paraesthetic area were significant. Discussion In this prospective follow-up study with 100 consecutive patients and 134 treated saphenous veins a high occlusion rate of 100% could be demonstrated one year after treatment. However, with LEED > 100 J/cm in this study, the incidence of paresthesia rose significantly. Therefore it seems adequate to stay below 100 J/cm in the future as the occlusion rate was the same below and above 100 J/cm. Conclusion EVLA of GSV and SSV with a 1470 nm diode laser is a minimally invasive, safe and efficient therapy option with a high success rate.
The Influence of Surface Temperature on Rewetting Behavior During Immersion Quenching of Hollow and Solid Cylinders
Authors: Frerichs F. , Luebben Th. 
Published: January 2009

Abstract
Due to the Leidenfrost phenomenon, immersion quenching is a more or less uncontrolled task, because prediction of the appropriate rewetting process is difficult. During the rewetting process the highest heat transfer coefficient occurs. Knowledge of the rewetting behavior is therefore one of the most important points for prediction of the distribution of heat transfer coefficients during immersion cooling. Up to now, it has been assumed that for a given set of experimental parameters, the rewetting process occurs when the surface temperature decreases below a given temperature. In the literature this temperature is called the Leidenfrost temperature. In this contribution, this assumption will be investigated by means of different kind of hollow and non-hollow cylinders. Those workpieces offer the possibility to investigate different distributions of surface temperatures and cooling rates. The observation of the rewetting process was done by means of high resolution video cameras. The investigations show clearly that for a given set of experimental parameters the rewetting process is not only a function of temperature. The progressive motion of the rewetting front is more a self-adjusting process which starts mostly on edges or surface failures of the workpiece.
Analysis of the Segerberg Hardening Power Equation
Authors: Chun-huai Chen , Jing-en Zhou 
Published: January 2009

Abstract
Cooling curves of ten different quenching oils were obtained and the hardness of test specimens quenched in these oils was determined. It was shown that the hardening power calculated from the Segerberg equation provided better agreement with test results than the transition temperature from vapor stage (full-film boiling) to the nucleate boiling stage and the maximum cooling rate. The hardening power equation also provided better agreement with respect to hardness for carbon steel than for alloy steel. The development of revised hardening power equations for carbon and alloy steels showed that there is an alloy-dependent optimal quenching temperature range, and it was concluded that the newly developed equations reported here are superior to the single equation previously published by Segerberg to quantify the quench severity provided by a quenching oil. A methodology to assess data set correlation is proposed and illustrated.
Structures of aluminium-based light weight hydrides
Authors: Hauback Björn C. 
Published: January 2009

Abstract
Light weight hydrides based on aluminium are among the most promising materials for hydrogen storage applications since the hydrogen content is up to 11 wt%. The crystal structures of many promising aluminium-based compounds have been determined and refined during the last years. This review gives an overview of structures of tetra-alanates (with AlH4 tetrahedra), hexa-alanates (with AlH6 octahedra) and different modifications of AlH3. The structure determinations are based on powder diffraction with X-rays, synchrotron X-rays and neutrons. In most structural work neutron diffraction has been used in order to determine accurate hydrogen/deuterium positions. In addition, density functional theory has been used both to predict new structures among these compounds and in combination with experimental work.
Crystal chemistry and metal-hydrogen bonding in anisotropic and interstitial hydrides of intermetallics of rare earth (R) and transition metals (T), RT3 and R2T7
Authors: Yartys Volodymyr A. , Vajeeston Ponniah , Riabov Alexander B. , Ravindran Ponniah , Denys Roman V. , Maehlen Jan Petter , Delaplane Robert G. , Fjellvåg Helmer 
Published: January 2009

Abstract
Hydrides of the i
3- and R2Ni7-based (R = light rare earth element) intermetallics exhibit novel structural features. Structures of these hydrides, including CeNi3D2.8, La2Ni7D6.5, LaNi3D2.8, and Ce2Ni7D4.7, are formed via a huge volume expansion occurring along a single crystallographic direction. Unique structural features during the formation of the hydrides include: (a) The lattice expansion proceeds exclusively within the RNi2 slabs leaving the RNi5 slabs unmodified. Such expansion, about 60% along [001] for the Laves layers, is associated with occupation by D atoms of these slabs; (b) New types of interstitial sites occupied by D are formed; (c) An ordered hydrogen sublattice is observed. In the present work we give (a) a review of the crystal chemistry of the conventional, interstitial type hydrides formed by RT3 and R2T7 intermetallic compounds (R = rare earths; T = Fe, Co, Ni) as compared to the unusual features of the crystal chemistry of anisotropic hydrides formed by the RNi3 and R2Ni7 intermetallics and (b) studies of the interrelation between structure and bonding in anisotropic hydrides by performing density functional calculations for CeNi3 and Ce2Ni7 intermetallic alloys and their corresponding hydrides. These studies allowed obtaining an understanding of the bonding mechanism in the hydrogenated compounds which causes a complete anisotropic rebuilding of their structures. From DOS analysis, both initial intermetallics and their related hydrides were found to be metallic. Bader topological analysis for the non-hydrogenated intermetallics showed that Ce atoms donate in average of almost 1.2 electrons to the Ni sites. Hydrogenation increases electron transfer from Ce; its atoms donate 1.2–1.6 electrons to Ni and H. Charge Density Distribution and Electron Localization Function for Ce2Ni7D4.7 phase clearly confirm that the interaction between the Ce and Ni does not have any significant covalent bonding. Ni is bonded with H via forming spatial frameworks –H–Ni–H–Ni– where H atoms accumulate an excess electron density of about 0.5e–. Thus, the tetrahedral or open saddle-type NiH4 coordination observed in the structures of the hydrides is not associated with the formation of [Ni0H4
1–]4– complexes containing a hydrido-ion H–1. In the structural frameworks there are terminal bonds Ni–H, bridges Ni–H–Ni, and the bonds where one H is bound to three different Ni. These spatial ordered frameworks stand as the principal reason for the anisotropic changes in the structural parameters on hydrogenation. Another unique feature of anisotropic hydrides is the donation of electrons from nonhydrogenated RNi5 parts to hydrogen in RNi2 slabs stabilising these fragments.