bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening for hemochromatosis by measuring ferritin levels: a more effective approach
Authors: Waalen Jill , Felitti Vincent J. , Gelbart Terri , Beutler Ernest 
Published: November 2007

AbstractBecause the penetrance of HFE hemochromatosis is low, traditional population screening measuring the transferrin saturation is unlikely to be cost-effective because the majority of subjects detected neither have clinical disease nor are likely to develop it. Three independent studies show that only patients with serum ferritin concentrations more than 1000 μg/L are at risk for cirrhosis, one of the main morbidities of hemochromatosis. Among 29 699 white subjects participating in the Scripps/Kaiser hemochromatosis study, only 59 had serum ferritin levels more than 1000 μg/L; 24 had homozygous mutant or compound heterozygous mutant HFE genotypes. In all but 5 of the other subjects, the causes of elevated ferritin were excessive alcohol intake, cancer, or liver disease. Screening for hemochromatosis with serum ferritin levels will detect the majority of patients who will be clinically affected and may detect other clinically significant disease in patients who do not have hemochromatosis genotypes. Because the ferritin level of the majority of adult homozygotes for HFE mutations does not rise over long periods of time, excluding subjects with serum ferritin levels less than or equal to 1000 μg/L should not result in missed opportunities for early treatment of patients who could benefit.
The Firegoose: two-way integration of diverse data from different bioinformatics web resources with desktop applications
Authors: Bare J Christopher , Shannon Paul T , Schmid Amy K , Baliga Nitin S 
Published: November 2007

Abstract

Background
Information resources on the World Wide Web play an indispensable role in modern biology. But integrating data from multiple sources is often encumbered by the need to reformat data files, convert between naming systems, or perform ongoing maintenance of local copies of public databases. Opportunities for new ways of combining and re-using data are arising as a result of the increasing use of web protocols to transmit structured data.

Results
The Firegoose, an extension to the Mozilla Firefox web browser, enables data transfer between web sites and desktop tools. As a component of the Gaggle integration framework, Firegoose can also exchange data with Cytoscape, the R statistical package, Multiexperiment Viewer (MeV), and several other popular desktop software tools. Firegoose adds the capability to easily use local data to query KEGG, EMBL STRING, DAVID, and other widely-used bioinformatics web sites. Query results from these web sites can be transferred to desktop tools for further analysis with a few clicks.
Firegoose acquires data from the web by screen scraping, microformats, embedded XML, or web services. We define a microformat, which allows structured information compatible with the Gaggle to be embedded in HTML documents.
We demonstrate the capabilities of this software by performing an analysis of the genes activated in the microbe Halobacterium salinarum NRC-1 in response to anaerobic environments. Starting with microarray data, we explore functions of differentially expressed genes by combining data from several public web resources and construct an integrated view of the cellular processes involved.

Conclusion
The Firegoose incorporates Mozilla Firefox into the Gaggle environment and enables interactive sharing of data between diverse web resources and desktop software tools without maintaining local copies. Additional web sites can be incorporated easily into the framework using the scripting platform of the Firefox browser. Performing data integration in the browser allows the excellent search and navigation capabilities of the browser to be used in combination with powerful desktop tools.
A SAGE-based screen for genes expressed in sub-populations of neurons in the mouse dorsal root ganglion
Authors: Bourane Steeve , Méchaly Ilana , Venteo Stéphanie , Garces Alain , Fichard Agnes , Valmier Jean , Carroll Patrick 
Published: November 2007

Abstract

Background
The different sensory modalities temperature, pain, touch and muscle proprioception are carried by somatosensory neurons of the dorsal root ganglia. Study of this system is hampered by the lack of molecular markers for many of these neuronal sub-types. In order to detect genes expressed in sub-populations of somatosensory neurons, gene profiling was carried out on wild-type and TrkA mutant neonatal dorsal root ganglia (DRG) using SAGE (serial analysis of gene expression) methodology. Thermo-nociceptors constitute up to 80 % of the neurons in the DRG. In TrkA mutant DRGs, the nociceptor sub-class of sensory neurons is lost due to absence of nerve growth factor survival signaling through its receptor TrkA. Thus, comparison of wild-type and TrkA mutants allows the identification of transcripts preferentially expressed in the nociceptor or mechano-proprioceptor subclasses, respectively.

Results
Our comparison revealed 240 genes differentially expressed between the two tissues (P < 0.01). Some of these genes, CGRP, Scn10a are known markers of sensory neuron sub-types. Several potential markers of sub-populations, Dok4, Crip2 and Grik1/GluR5 were further analyzed by quantitative RT-PCR and double labeling with TrkA,-B,-C, c-ret, parvalbumin and isolectin B4, known markers of DRG neuron sub-types. Expression of Grik1/GluR5 was restricted to the isolectin B4+ nociceptive population, while Dok4 and Crip2 had broader expression profiles. Crip2 expression was however excluded from the proprioceptor sub-population.

Conclusion
We have identified and characterized the detailed expression patterns of three genes in the developing DRG, placing them in the context of the known major neuronal sub-types defined by molecular markers. Further analysis of differentially expressed genes in this tissue promises to extend our knowledge of the molecular diversity of different cell types and forms the basis for understanding their particular functional specificities.
Factors associated with the appropriate use of preoperatory hospital stays: historical cohort study
Authors: Tamames Sonia , Perez Rubio Alberto , Castrodeza Sanz Javier , Canton Alvarez Maria Belen , Luquero Francisco J , Santos Sanz Sara , Lopez Encinar Placido , de la Torre Pardo Maria Paz , Gil Gonzalez Juan Manuel 
Published: November 2007

Abstract

Background
To ensure the highest efficiency, health services should be provided with the least possible complexity. The aim of this study is to quantify the degree of appropriateness in preoperatory hospital stays and to analyse those factors associated with a greater inappropriate use.

Methods
Historical cohort study. The histories of 440 hospitalised patients who underwent at least one surgical procedure were analysed. Data collection was carried out by doctors not involved in the services studied, following the Appropriateness Evaluation Protocol. A bivariate and multivariate analysis of the factors associated with the appropriateness of preoperatory stays was carried out.

Results
The mean number of days of preoperatory stay was 5.5 (SD 5.11), of which a mean number of 2.5 days were considered to be inappropriate (SD 4.11). The overall rate of inappropriateness was 45.2% (CI 95% 43.3–47.1). The multivariate analysis showed a positive association of the inappropriateness of the preoperatory stay with weekend days, programmed admission, hospital stays longer than 7 days, medical records incorrectly or incompletely documented and the age groups of 45–65 and the >65 with respect to the <45 age group. Sex and an incorrect or incomplete nursing register did not show such an association.

Conclusion
The inappropriate use of hospital stay during preoperatory care affects almost half the period and there are some risk determinants that could act as indicators at admission. In addition, the efficiency of care provision was found to vary greatly from the point of view of its appropriateness.
Using hospital discharge data for determining neonatal morbidity and mortality: a validation study
Authors: Ford Jane B , Roberts Christine L , Algert Charles S , Bowen Jennifer R , Bajuk Barbara , Henderson-Smart David J ,   the NICUS group
Published: November 2007

AbstractBackgroundDespite widespread use of neonatal hospital discharge data, there are few published reports on the accuracy of population health data with neonatal diagnostic or procedure codes. The aim of this study was to assess the accuracy of using routinely collected hospital discharge data in identifying neonatal morbidity during the birth admission compared with data from a statewide audit of selected neonatal intensive care (NICU) admissions.MethodsValidation study of population-based linked hospital discharge/birth data against neonatal intensive care audit data from New South Wales, Australia for 2,432 babies admitted to NICUs, 1994–1996. Sensitivity, specificity and positive predictive values (PPV) with exact binomial confidence intervals were calculated for 12 diagnoses and 6 procedures.ResultsSensitivities ranged from 37.0% for drainage of an air leak to 97.7% for very low birthweight, specificities all exceeded 85% and PPVs ranged from 70.9% to 100%. In-hospital mortality, low birthweight (≤1500 g), retinopathy of prematurity, respiratory distress syndrome, meconium aspiration, pneumonia, pulmonary hypertension, selected major anomalies, any mechanical ventilation (including CPAP), major surgery and surgery for patent ductus arteriosus or necrotizing enterocolitis were accurately identified with PPVs over 92%. Transient tachypnea of the newborn and drainage of an air leak had the lowest PPVs, 70.9% and 83.6% respectively.ConclusionAlthough under-ascertained, routinely collected hospital discharge data had high PPVs for most validated items and would be suitable for risk factor analyses of neonatal morbidity. Procedures tended to be more accurately recorded than diagnoses.
Phase II Trial of a Transplantation Regimen of Yttrium-90 Ibritumomab Tiuxetan and High-Dose Chemotherapy in Patients With Non-Hodgkin's Lymphoma
Authors: Krishnan Amrita , Nademanee Auayporn , Fung Henry C. , Raubitschek Andrew A. , Molina Arturo , Yamauchi Dave , Rodriguez Roberto , Spielberger Ricardo T. , Falk Peter , Palmer Joycelynne M. , Forman Stephen J. 
Published: November 2007

Purpose This phase II trial evaluated the safety and efficacy of combining yttrium-90 (90Y) ibritumomab tiuxetan with high-dose carmustine, cytarabine, etoposide, and melphalan (BEAM) and autologous stem-cell transplantation in patients with non-Hodgkin's lymphoma who were considered ineligible for total-body irradiation because of older age or prior radiotherapy. Patients and Methods Between May 2002 and January 2006, 14 days before autologous stem-cell transplantation, 41 patients with non-Hodgkin's lymphoma received standard-dose 90Y ibritumomab tiuxetan (14.8 MBq/kg [0.4 mCi/kg]) followed by high-dose BEAM. Results The median age was 60 years (range, 19 to 78 years), and the median number of previous therapies was two (range, one to six). Disease histologies were diffuse large B-cell (n = 20), mantle cell (n = 13), follicular (n = 4), and transformed lymphoma (n = 4). With a median follow-up of 18.4 months (range, 5.5 to 53.3 months) the estimated 2-year overall and progression-free survival were 88.9% (95% CI, 75.3% to 95.2%) and 69.8% (95% CI, 56.4% to 79.7%). The median time to WBC engraftment was 11 days (range, 9 to 26 days) and time to platelet engraftment was 12 days (range, 3 to 107 days). Adverse events were similar to those seen historically with high-dose BEAM alone, and included grade 3 or 4 pulmonary toxicity in 10 patients. Conclusion Adding 90Y ibritumomab tiuxetan to high-dose BEAM with autologous stem-cell transplantation is feasible and has a toxicity and tolerability profile similar to that observed with BEAM alone. Rates of progression-free survival seen in these patients are promising and warrant additional study.
Hydrothiolation of Unactivated Alkynes Catalyzed by Indium(III) Bromide
Authors: Yadav J S , Reddy B V Subba , Raju A , Ravindar K , Baishya Gakul 
Published: November 2007

Abstract
Indium(III) bromide is found to catalyze efficiently the hydrothiolation of unactivated alkynes with various thiols under mild conditions to produce the corresponding dithioacetals in excellent yields. However, addition of aromatic thiols on aromatic alkynes gave the β-vinyl sulfides in high yields. This new procedure offers significant advantages such as high conversions, short reaction times, and simplicity in operation.
Synthetic Approach towards the Sex Pheromone of the Female Oleander Scale Aspidiotus Nerii
Authors: Schmidt Arndt W , Suresh Joghee R , Theumer Gabriele , Knölker Hans-Joachim 
Published: November 2007

Abstract
We report a synthetic approach to the sex pheromone of the female oleander scale Aspidiotus nerii using our Lewis acid promoted [2 + 2] cycloaddition of allylsilanes in combination with the modified Fleming–Tamao oxidation.
Synthetic Studies on Mycalolide B: Synthesis of the C7–C35 Fragment
Authors: Kimura Tomoyuki , Kuribayashi Satomi , Sengoku Tetsuya , Matsui Keita , Ueda Shoko , Hayakawa Ichiro , Suenaga Kiyotake , Kigoshi Hideo 
Published: November 2007

Abstract
Mycalolide B, a cytotoxic macrolide isolated from a marine sponge of the genus Mycale, has an actin depolymerizing activity. This paper reports the synthesis of C7–C35 fragment of mycalolides via cross olefin metathesis reaction between trisoxazole fragment and side-chain fragment.
2H- and 13C-Labelling Studies on Skeletal Reorganization of 1,6-Enynes
Authors: Nakai Hiromi , Chatani Naoto 
Published: November 2007

Abstract
2H- and 13C-labelling studies on skeletal reorganization of 1,6-enynes having a terminal alkyne moiety have been performed with various catalysts. The products are 1-vinylcyclopentenes, but two possible isomers, type I and II products, can be formed. The formation of type I involves the cleavage of the original C–C double bonds and migration of the terminal alkene carbon atom to the terminus of the alkyne. On the other hand, the formation of type II involves a double cleavage of the C–C double bond and the C–C triple bond, which is an anomalous bond connection. The product ratio is affected by the nature of the catalyst used. Type II is obtained as a major isomer in the case of late transition metal halides. On the other hand, the type I product forms exclusively in the presence of a typical element halide, such as InCl3.
DFT Calculation of Site-specific Acid Dissociation Constants of Purine Nucleobases
Authors: Jang Yun Hee , Hwang Sungu , Chung Doo Soo 
Published: November 2007

Abstract
The intrinsic proton affinities of individual basic sites, so-called micro acid dissociation constants, of purine nucleobases in aqueous solution are calculated using an ab initio quantum-mechanical method in combination with the Poisson–Boltzmann continuum solvation model. Calculated micro acid dissociation constants as well as macro acid dissociation constants (pKa) agree with recent experimental measurements mostly within a pH unit.
The Perception of Poverty in Northern Ireland
Authors: Ditch John 
Published: November 2007

This paper extracts data from the EEC’s public opinion survey entitled ‘The Perception of Poverty’ and presents previously unpublished data relating to Northern Ireland respondents. It suggests a relatively low awareness of the existence of poverty; a suspicion of state intervention to alleviate its causes and consequences; a tendency to attribute ‘need’ to individual or pathological rather than social causes. Differences in attitude and perception between Protestants and Catholics are also presented and discussed. Overall, respondents in Northern Ireland are closer in their attitudes to respondents in Great Britain than they are to respondents in the Republic of Ireland.
The data relates to 312 cases in the province but because of the characteristics of the survey and the high non-response rate to certain questions (for example, voting intentions) the discussion is entirely descriptive. This is recognised to be a major deficiency in the paper, but it is believed that in the absence of any comparable data, and as an exercise in exploration rather than explanation it does provide an illustrative basis for subsequent work using a more satisfactory survey.
Manufacturing Variability, Microstructure, and Deformation of Zr-2.5Nb Pressure Tubes
Authors: Bickel G. A. , Griffiths M. 
Published: November 2007

Abstract
The time-dependent deformation behavior of pressure tubes in CANDU™ (CANada Deuterium Uranium) reactors is an important property that has to be predicted for reactor life management. Measurements accumulated over many years have shown that there is considerable variability in deformation rates between different tubes. The deformation behavior not only varies from tube to tube but also varies along the length of each tube; this axial variation in itself is unique to each tube. The deformation behavior is determined by the microstructure and this is a function of the manufacturing history. An exhaustive study has been conducted to collect and compile deformation and manufacturing data for tubes in many different reactors. The manufacturing data have been analyzed with respect to the measured in-reactor performance of the pressure tubes. Data from pressure tubes fabricated over a 30-year period have been analyzed. During this time the manufacturing parameters have evolved to improve the workability and mechanical properties and also reduce tube-to-tube variability. The effect of manufacturing variables on in-reactor performance has been assessed for tubes that have been in service for many years and approaching the end of their design life. The analysis shows that the two most important factors that affect the deformation behavior of pressure tubes are the material source (ingot) and the extrusion conditions. These are related by the microstructure (texture, grain size, and dislocation density) to the deformation. The results of the statistical analyses will be presented and discussed in terms of the manufacturing conditions that bring about specific microstructures.
The effects of affect and input source on flashbulb memories
Authors: Bohannon John Neil , Gratz Sami , Cross Victoria Symons 
Published: November 2007

AbstractFlashbulb memories of shocking news (Challenger Explosion, death of Princess Diana, Pearl Harbour and Iraq bombings) were employed to test two hypotheses: encoding emotion enhances memory and how one gets the shocking news will help determine the nature of what is recalled. Different groups of subjects (Total = 2405) remembered their discoveries at delays ranging from 2 weeks to 50 years on three memory measures: a free and probed recall test of their flashbulb discovery, and a probed recall of the facts concerning the events themselves. Subjects were grouped according to the source of their discovery (Media vs. Person), affect at encoding (calm vs. upset) and recounts (few vs. many). The results indicated that how one learns of shocking news determined the type and extent of the resultant memory. ‘Media’ subjects remembered more facts whereas ‘person’ subjects recalled more of their personal discoveries regardless of the initial flashbulb inspiring event. Copyright © 2007 John Wiley & Sons, Ltd.
Catalytic‐Kinetic Determination of Silver(I) Using Hexacyanoferrate(II) and 2,4,6‐Tripyridyl‐1,3,5‐Triazine(TPTZ)
Authors: Reddy K.P.P.R. Mohan , Chowdary P. Govind , Reddy V. Krishna , Reddy P. Raveendra 
Published: November 2007

AbstractA highly sensitive and selective kinetic spectrophotometric method is proposed for the determination of silver in nanogram quantities. The method is based on catalytic activity of silver(I) in enhancing the green colour formation between potassium ferrocyanide and 2,4,6‐tripyridyl‐1,3,5‐triazine (TPTZ) which is otherwise a very slow reaction at pH 2.0. The absorbance spectrum of the green coloured solution in presence of silver showed two peaks of almost equal intensity one at 405 nm and another at 600 nm. At both the wavelengths the absorbance of the experimental solution increased proportionally with the amount of silver(I) in the range 5 – 410 ng mL‐1 and 10 – 610 ng mL‐1 respectively. Other experimental conditions like pH, reagent concentration, ferrocyanide concentration and effect of time for obtaining maximum reaction rate were optimized. The molar absorptivity, Sandell's sensitivity, detection limit, determination limit and relative standard deviation (n = 8) were determined as 1.57 × 106 L mol‐1 cm‐1, 0.065 ng cm‐2, 1.6 ng mL‐1, 4.8 ng mL‐1 and 0.81 % respectively. The effect of various diverse ions on the determination of silver was studied. The method was applied for the determination of silver in alloys, biological samples, effluents, real water and in ayurvedic medicines.
A Modified Glassy Carbon Electrode for Hydrogen Peroxide Sensing
Authors: Zheng Cai‐yun , Chen Shui‐hong , Shang Yong‐jia , Li Mao‐guo 
Published: November 2007

AbstractIn this paper, 4‐ferrocenyl benzoic acid (FcBA) was synthesized and the electrochemical behaviors of the synthesized ferrocenyl derivative were studied; Then, the multi‐wall carbon nanotubes (MWCNTs) coated FcBA modified glassy carbon (MWCNTs/FcBA/GC) electrode was prepared conveniently and the as‐prepared modified electrode was characterized with both electrochemical impedance spectroscopy (EIS) and transmission electron microscopy (TEM). The results suggested that FcBA could coat the MWCNTs tightly in dimethylformamide (DMF) and the coated MWCNTs could be strongly adsorbed on the surface of glass carbon (GC) electrode. The MWCNTs/FcBA/GC electrode exhibited an excellent electrocatalytic activity toward the reduction of hydrogen peroxide. The modifiedelectrode was hence used for the determination of hydrogen peroxide by differential pulse voltammetry (DPV). The linear range of the modified electrode was 2.5×10‐6 and 5.0×10‐4 mol/l, and the detection limit was estimated to be of 4.8×10‐7 mol/l when the signal‐to‐noise ratio was 3. Further studies indicated that the modified electrode was of a fast response, high sensitivity, good reproducibility, and long‐term stability.
Characterization of Activated Carbon and Application of Copper Removal from Drinking Water
Authors: Kalpakli Yasemen Kutmen , Koyuncu İkbal 
Published: November 2007

AbstractIn this study, removal of copper ions from aqueous solution by adsorption was investigated. The methods were applied to removal of copper in artificial water and drinking water samples using activated carbon. For this purpose, metal removal from water depends on the pH, initial concentration of metal, amount of activated carbon, and effect of sorption time were investigated. The process parameters were investigated with batch studies. In this study, commercial activated carbon, Chemviron C‐1300, has been used. Granular activated carbon were characterized using nitrogen porosimetry, pH titration, Boehm's titration, mercury porosimetry, FTIR and scanning electron microscope measurements. The copper ions were determined by an atomic absorption spectrometry. Optimum conditions were found as concentration of 10 mg l‐1, pH: 5, contact time:25 min, and activated carbon amount:750 mg. Optimum condition were applied to drinking water for removal of Cu(II). Furthermore, adsorption isotherm data were fitted to both Langmuir and Freundlich models for finding the optimum conditions.