bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in the rust resistance of greenhouse and outdoor‐grown white birch species, Betula spp.
Authors: Poteri M. , Rousi M. , Gao Zhi‐He 
Published: June 2007

SummaryThe 2‐year‐old seedlings of five different white birch species (Betula platyphylla, Betula papyrifera, Betula pubescens, Betula pendula (two types) and Betula resinifera x Betula pendula) grown both in a greenhouse and outdoors, were inoculated in a leaf disc assay with two different birch rust (Mel‐ampsoridium betulinum) isolates from B. pendula and B. pubescens. The resistance of these birch species varied significantly. Resistance to the B. pubescens rust isolate was not related to the resistance of the B. pendula rust isolate. The behaviour of a birch genotype grown in the greenhouse did not correspond to the behaviour of the same genotype grown outdoors.The outdoor growth environment greatly increased the contents of soluble proteins, rubisco, chloro‐phyll and nitrogen in the leaves of diploid birch species (B. platyphylla, B. pendula and B. resinifera x B. pendula). For tetraploid and pentaploid species (B. pubescens and B. papyrifera, respectively) there was no such clear difference in the leaf physiological status between the seedlings grown outdoors and in the greenhouse. The C:N ratio was higher for the greenhouse‐grown seedlings in all the birch species, but the difference was significant only with the diploid species. The incidence of rust in the birch species did not correlate with any of the leaf physiological parameters studied. The adaptability of birch genotypes to the environment in relation to their resistance to birch leaf rust is discussed.
Vegetative compatibility and pathogenicity of Diplodia mutila isolates on oak
Authors: Ragazzi A. , Moricca S. , Dellavalle I. 
Published: June 2007

SummaryForty isolates of Diplodia mutila, a dieback agent on oaks were characterized by their capacity to produce heterokaryons, by their vegetative compatibility, and by their pathogenicity tests on Quercus cerris. The forty isolates were collected from Quercus cerris, Quercus frainetto, Quercus ilex, Quercus pubescens, Quercus robur and Quercus suber in different regions in Italy. Three nit mutants (nitt, nit and nitM) were identified with different nitrogen sources: sodium nitrite, sodium nitrate, uric acid, ammonium tartrate and hypoxanthine. On the two nutrient media employed, a potato dextrose agar (PDA) medium with chlorate (PDC), and a minimal agar medium with chlorate (MMC), isolates obtained from Q. cerris yielded the greatest number of mutants: 254 on PDC (out of a total of 583) and 190 on MMC (out of a total of 440). Nine vegetative compatibility groups (VCGs) were identified. There was a strong association between these VCGs and host species. All isolates tested except one from Q. cerris were pathogenic.
Isozyme and RAPD polymorphisms in Heterobasidion annosum in Italy
Authors: Goggioli V. , Capretti P. , Hamelin R. C. , Vendramin G. G. 
Published: June 2007

SummaryIsozyme and random amplified polymorphic DNA (RAPD) polymorphisms were used to study variability in a group of 41 isolates from the Italian population of Heterobasidion annosum. The isolates belonged to the intersterility groups P and S, and particularly to the group that is most widely distributed in Italy, group F. Isozyme analysis was effective in identifying the three intersterility groups and revealed a high degree of genetic divergence within the P group isolates; the mannose phosphate isomerase (MPI‐2) locus was diagnostic in the attribution of isolates to the more correlated F and S groups. RAPDs were detected following amplification by the polymerase chain reaction (PCR). 74 RAPD fragments, obtained through amplifications with eight primers, were scored. Isolates from the 3 intersterility groups were clearly divergent based on analysis of RAPD markers. However, a similarity index calculated for the isolates within the F population indicated a high uniformity of the isolates collected throughout the Italian peninsula.
Genetic variation in three Ceratocystis species with outcrossing, selfing and asexual reproductive strategies
Authors: Harrington T. C. , Steimel J. , Kile G. 
Published: June 2007

SummaryNuclear and mitochondrial markers were used to examine variation within three closely related species of tree pathogens with differing reproductive strategies. Ceratocystis eucalypti is obligately outcrossing; Ceratocystis virescens is capable of selfing due to unidirectional mating type switching; and Chalara australis is an asexual species, comprised of a single mating type. When the nuclear DNA fingerprinting markers (CAT)5 and (CAG)5 were used as probes against Pst I‐restricted DNA, isolates of C. eucalypti were found to be highly polymorphic, and Ch. australis showed very little polymorphism. The selfing C. virescens showed an intermediate level of variation in the nuclear fingerprint markers, and much of the variation appeared to be due to differences between two forms of the species, one pathogenic to Acer and Liriodendron and another less‐pathogenic form on Fagus and other hardwoods. Mitochondrial DNA (mtDNA) polymorphisms were examined by digesting total DNA with Hae III or CfoI, and C. eucalypti showed somewhat more variation in mtDNA than did C. virescens. The only polymorphism seen in the mtDNA of Ch. australis was associated with a plasmid. Selfing in C. virescens may be common and could explain an intermediate level of diversity when compared to the obligately outcrossing C. eucalypti and the asexual Ch. australis.
Rust scorings in a plantation of Salix viminalis clones during ten consecutive years
Authors: ÅHman I. 
Published: June 2007

SummaryAmong pests and diseases occurring in coppiced willow plantations established to produce biofuel, Melampsora rust is the most widespread and severe. In the present study, the variation in rust attacks in a set of Salix viminalis clones was investigated. The aim was to look for trends possibly arising from changes in rust genotype composition, to get some idea about the risks of rust overcoming resistance bred into new willow varieties. Rust levels on 92 S. viminalis clones belonging to 18 different families were scored once each year during 1987–96 in a plantation in southern Sweden. Rust scorings in 1993 deviated most from the average scoring pattern of the clones. Furthermore there was a successive change, such that the longer the time interval between scorings the lower was the correlation between rust scores. Willow families were identified that deviated from the others over time. Various explanations for inconsistency in rust scores over time are discussed.
Effects of host plant hybridization on resistance to willow leaf rust caused by Melampsora sp.
Authors: Roche B. M. , Fritz R. S. 
Published: June 2007

SummaryPatterns of resistance to willow leaf rust caused by Melampsora sp. were measured on Salix eriocephala, Salix sericea, and their interspecific hybrids in natural field populations in 1991–94 and on potted plants in common garden experiments in 1994 and 1995. Testing was carried out to establish if the resistance of hybrids was intermediate between pure parental species (additive), equal to one of the parents (dominant), lower than both parents (susceptible), higher than both parents (resistant), or not different than either parent (null hypothesis). Support was generally found for hybrid susceptibility in the field and common garden experiments, with the null hypothesis of no difference having some support. The results from the common garden study suggest that the susceptibility of hybrids seen in the field is due to genetic differences. Hybridization between these species of willows results in increased susceptibility to this pathogen. The variation among years in the relative susceptibility of hybrid and parental willows may be due to environmental effects on the willows or to shifts in the abundance of Melampsora races.
Life‐cycles and ecology of willow beetles on Salix viminalis in England
Authors: Kendall D. A. , Wiltshire C. W. 
Published: June 2007

SummaryThe life‐cycles of three willow beetle pests, Phratora (= Phyllodecta) vulgatissima, Phratora (=Phyllodeeta) vitellinae and Galerucella lineola (Coleoptera, Chrysomelidae), were investigated during 1994–95 in a plantation of short‐rotation coppiced willows (Salix viminalis cv. Bowles Hybrid) at Long Ashton, Bristol, UK. The P. vulgatissima had one generation during the year. Overwintered adults emerged from hibernation in April and after a short feeding period, copulation and egg‐laying occurred. Larval stages were found from May–July. The new generation of adult beetles appeared in July–August and fed for a while before hibernating. Hibernating adults were found in hedgerows around the site and in the plantation on willow stools and ground vegetation. Dispersal of adult beetles between feeding and overwintering sites appeared to be fairly localized. Hence, crop management strategies that reduce the potential for overwintering in and around plantations may help to minimize spring re‐invasion and damage. Although based on limited observations, the biology of P. vitellinae and G. lineola appeared similar to that of P. vulgatissima. However, there is evidence in the literature that both these species, unlike the latter, can have a partial second generation in some years. The potential effect of insect herbivory on the growth and biomass yield of S. viminalis cv. Bowles Hybrid was investigated experimentally by artificial hand‐defoliation of pot‐grown plants. Yield losses were strongly correlated with the amount and time of defoliation. Results indicated that even slight or moderate damage by insect herbivores could have a significant impact on the biomass productivity of coppiced willows.
Rapid living crown reduction caused by Gremmeniella abietina affects foliar nutrient concentrations of Scots pine
Authors: Nuorteva H. , Kurkela T. , Lehto A. 
Published: June 2007

SummaryThe foliar chemistry of diseased and healthy trees was studied one growing season after severe reduction in living crown caused by Gremmeniella abietina in four young Scots pine (Pinus sylvestris) stands. Sample trees were chosen pairwise on the basis of the living crown length: a diseased tree with about 50% live crown reduction and a healthy tree in each pair. Fifteen elements were determined in the youngest healthy needles on the lateral top shoots of each sample tree. Diseased trees had higher foliar boron, manganese and sodium concentrations and lower magnesium, iron, zinc, copper, potassium, nitrogen and sulphur concentrations compared to the healthy trees. Foliar calcium, aluminium, phosphorus, carbon and hydrogen concentrations did not differ between the diseased and healthy trees, except for P and A1 in two of the stands when the stands were analysed separately. Significant correlations between the needle element concentrations and crown ratio (length of the living crown/tree height) were found especially for B (increasing B with decreasing crown ratio) and for Mg, Fe and Zn (decreasing concentrations with decreasing crown ratio). The effect of G. abietina‐induced living crown reduction on tree nutrient status and the role of these mineral nutrients in the susceptibility are discussed.
Genetic variation of the resin top fungus in Finland as determined by random amplified microsatellites (RAMS)
Authors: Hantula J. , Niemi E. Maarit , Kaitera J. , Jalkanen R. , Kurkela T. 
Published: June 2007

SummaryThe analysis of random amplified microsatellite (RAMS) markers among aecia of the causal agent of the resin top disease (Peridermium pini) on Scots pine (Pinus sylvestris) suggested that the genetic variation between the populations of this rust fungus is low in Finland. The method used allowed the identification of heterozygotic aecia in two loci, where the degree of heterozygosity was, however, low. The RAMS patterns of the Finnish aeciospores, other aeciospores from Thetford, UK and Cronartium flaccidum from Italy were highly similar suggesting that the autoecious and heteroecious forms of these rust fungi would be genetically closely related.
Snow‐packing as a potential harmful factor on Picea abies, Pinus sylvestris and Betula pubescens at high altitude in northern Finland
Authors: Jalkanen R. , Konocpka B. 
Published: June 2007

SummarySnow‐packing, a combination of ice, hoarfrost and snow on trees, and the subsequent tree damage by snow, were estimated on Norway spruce (Picea abies), Scots pine (Pinus sylvestris), and pubescent birch (Betula pubescens) in the winter of 1993‐94 in southern Lapland, northern Finland, near the local alpine timberline around 400 m a.s.l. Snow‐packing on each fully sized tree increased from a few hundred kilograms at 150–250 m a.s.l. to a maximum of 3290 kg at 300–350 m a.s.l. At 300 m a.s.l., snow‐packing per metre of stem increased from 30 to 50 kg on trees < 5 m in height up to 180–200 kg on 20‐m trees. There was 300 000‐480 000 kg/ha of snow accumulated on tree crowns. No stem breakage by snow‐packing occurred at or below 250 m a.s.l., whereas at 290–350 m a.s.l., 0‐46%, 39‐100%, and 0–33% of the spruce, pine and birch trees, respectively, had broken tops. Birch appeared to be the most resistant and pine the most susceptible to snow breakage.
Effects of Ophiostoma ulmi and Ophiostoma novo‐ulmi culture filtrates on elm cultures from genotypes with different susceptibility to Dutch elm disease
Authors: Diez J. , Gil L. 
Published: June 2007

SummaryCell cultures of callus tissue cultures obtained from four elm genotypes (Ulmus minor; Ulmus minor×Ulmus pumila; [Ulmus carpinifolia×Ulmus glabra] × [Ulmus wallichiana×Ulmus glabra]; and Ulmus pumila), either susceptible or resistant to Dutch elm disease (DED) were exposed to culture filtrates of Ophiostoma ulmi and Ophiostoma novo‐ulmi. Elm cells were largely affected by crude culture filtrate incorporated into the media. However, the correlation between ‘in vivo’ cell resistance and growth in the presence of culture filtrate was poor: the effects of fungal media components were greater than that exerted by fungal exotoxins. Therefore, it is concluded that these ‘in vitro’ assays cannot be used to evaluate resistance sources to DED in elms, or to assess specific pathogenicity of fungal isolates.
Tiarosporella durmitorensissp. nov. – a new pathogenic fungus on needles ofAbies alba
Authors: Karadži D. M. 
Published: June 2007

SummaryA fungus causing necrosis and needle cast has been discovered on fir trees (Abies alba) in a hight of 1700 m above sea level in Yugoslavia. The intensity of infection was very high, and the needles of all age classes were infected. The consequence of the infection is the death of the tree. The pathogen is described as a new speciesTiarosporella durmitorensisKaradžić. So far, this fungus has been found only in European silver fir stands in the National Park Durmitor and in the National Park Biogradska Gora (Montenegro).
Comparison of vegetative compatibility types in Italian and Swiss subpopulations of Cryphonectria parasitica
Authors: Cortesi P. , Rigling D. , Heiniger U. 
Published: June 2007

SummaryTester isolates of 20 vegetative compatibility (vc) types from 11 Italian subpopulations and tester isolates of 26 vc types from five Swiss subpopulations of Cryphonectria parasitica were compared by two different vc test methods. A total of 31 different vc types was identified; 15 vc types were common to both countries, five Italian vc types were not found in Switzerland and 11 Swiss vc types were not found in Italy. These 31 vc types were labelled with the acronym EU, followed by progressive numbers and may constitute the base for a common European nomenclature. The vc type EU‐2 was the most common vc type in both countries and dominated in all Swiss and in seven Italian subpopulations. The vc types EU‐1 and EU‐5 were found primarily in northern Italy and Switzerland, while EU‐10 and EU‐12 were most common in southern Italy. The diversity of vc types was greater in the subpopulations of Switzerland and northern Italy than in the subpopulations of southern Italy. The geographical distribution of vc types in Europe should therefore be taken into consideration for biological control measures of chestnut blight and quarantine regulations.
Investigating Burkholderia cepacia complex populations recovered from Italian maize rhizosphere by multilocus sequence typing
Authors: Dalmastri Claudia , Baldwin Adam , Tabacchioni Silvia , Bevivino Annamaria , Mahenthiralingam Eshwar , Chiarini Luigi , Dowson Christopher 
Published: June 2007

SummaryThe Burkholderia cepacia complex (BCC) comprises at least nine closely related species of abundant environmental microorganisms. Some of these species are highly spread in the rhizosphere of several crop plants, particularly of maize; additionally, as opportunistic pathogens, strains of the BCC are capable of colonizing humans. We have developed and validated a multilocus sequence typing (MLST) scheme for the BCC. Although widely applied to understand the epidemiology of bacterial pathogens, MLST has seen limited application to the population analysis of species residing in the natural environment; we describe its novel application to BCC populations within maize rhizospheres. 115 BCC isolates were recovered from the roots of different maize cultivars from three different Italian regions over a 9‐year period (1994–2002). A total of 44 sequence types (STs) were found of which 41 were novel when compared with existing MLST data which encompassed a global database of 1000 clinical and environmental strains representing nearly 400 STs. In this study of rhizosphere isolates approximately 2.5 isolates per ST was found, comparable to that found for the whole BCC population. Multilocus sequence typing also resolved inaccuracies associated with previous identification of the maize isolates based on recA gene restriction fragment length polymorphims and species‐specific polymerase chain reaction. The 115 maize isolates comprised the following BCC species groups, B. ambifaria (39%), BCC6 (29%), BCC5 (10%), B. pyrrocinia (8%), B. cenocepacia IIIB (7%) and B. cepacia (6%), with BCC5 and BCC6 potentially constituting novel species groups within the complex. Closely related clonal complexes of strains were identified within B. cepacia, B. cenocepacia IIIB, BCC5 and BCC6, with one of the BCC5 clonal complexes being distributed across all three sampling sites. Overall, our analysis demonstrates that the maize rhizosphere harbours a massive diversity of novel BCC STs, so that their addition to our global MLST database increased the ST diversity by 10%.
Use of a stress inducible promoter to drive ectopic AtCBF expression improves potato freezing tolerance while minimizing negative effects on tuber yield
Authors: Pino María‐Teresa , Skinner Jeffrey S. , Park Eung‐Jun , Jeknić Zoran , Hayes Patrick M. , Thomashow Michael F. , Chen Tony H. H. 
Published: June 2007

Summary Solanum tuberosum is a frost‐sensitive species incapable of cold acclimation. A brief exposure to frost can significantly reduce its yields, while hard frosts can completely destroy entire crops. Thus, gains in freezing tolerance of even a few degrees would be of considerable benefit relative to frost damage. The S. tuberosum cv. Umatilla was transformed with three Arabidopsis CBF genes (AtCBF1‐3) driven by either a constitutive CaMV35S or a stress‐inducible Arabidopsis rd29A promoter. AtCBF1 and AtCBF3 over‐expression via the 35S promoter increased freezing tolerance about 2 °C, whereas AtCBF2 over‐expression failed to increase freezing tolerance. Transgenic plants of AtCBF1 and AtCBF3 driven by the rd29A promoter reached the same level of freezing tolerance as the 35S versions within a few hours of exposure to low but non‐freezing temperatures. Constitutive expression of AtCBF genes was associated with negative phenotypes, including smaller leaves, stunted plants, delayed flowering, and reduction or lack of tuber production. While imparting the same degree of freezing tolerance, control of AtCBF expression via the stress‐inducible promoter ameliorated these negative phenotypic effects and restored tuber production to levels similar to wild‐type plants. These results suggest that use of a stress‐inducible promoter to direct CBF transgene expression can yield significant gains in freezing tolerance without negatively impacting agronomically important traits in potato.
‘SCHLECHTES MENSCH/GUTES OPFER’: THE ROLE OF MARIE IN GEORG BOCHNER'S WOYZECK
Authors: Martin Laura 
Published: June 2007

Readings of Woyzeck which seek to explain the eponymous character's behaviour as externally caused as well as those who see in him the epitome of solitary Man, alone in the universe, alike ignore the consequences of Woyzecks insanity and social subjugation: namely, the murder of Mane. Yet this murder is by no means the logical result of Woyzeck's abuse by his social superiors nor is it a solution to his existential loneliness.This feminist reading of Woyzeck draws on theories of the scapegoat and sacrifice (Girard) and on feminist cultural critiques (Irigaray and others) to reveal the culturally determined assumptions about sexuality, love, and revenge, which make the murder of a promiscuous woman both a necessary and an acceptable outcome of the play. It also proposes that the relevance of Büchner's play for the modern, and especially the woman, reader or spectator lies in its exploration of the production of such cultural assumptions, and not in their unquestioning reproduction.
Dissecting corollary discharge dysfunction in schizophrenia
Authors: Ford Judith M. , Gray Max , Faustman William O. , Roach Brian J. , Mathalon Daniel H. 
Published: June 2007

AbstractDuring talking, a corollary discharge prepares cortex for self‐generated sounds, minimizing responsiveness and providing a way to recognize sounds as self‐generated. When we talk, we are the agent producing the sound and know what sound to expect. The auditory ERP N1 is normally suppressed during talking, but less so in schizophrenia, perhaps due to deficits in agency and expectancy inherent to talking. N1 was assessed in 27 patients (23 schizophrenia, 4 schizoaffective) and 26 controls. During talking, subjects said “ah” every 1–2 s. During agency, subjects pressed a button to deliver “ah” every 1–2 s. During expectancy, “ah” followed a visual warning. Talking yielded greatest N1 suppression in controls and greatest suppression failure in patients. Agency and expectancy had modest suppression effects on N1 and only in controls. Group differences in expectancy and agency could not account for failed corollary discharge during talking in patients.
Optimal Cost Sampling for Decision Making with Multiple Regression Models
Authors: Eakin Mark , Schkade Lawrence L. , Whiteside Mary 
Published: June 2007

ABSTRACTThis paper develops an explicit relationship between sample size, sampling error, and related costs for the application of multiple regression models in observational studies. Graphs and formulas for determining optimal sample sizes and related factors are provided to facilitate the application of the derived models. These graphs reveal that, in most cases, the imprecision of estimates and minimum total cost are relatively insensitive to increases in sample size beyond n=20. Because of the intrinsic variation of the regression model, even if larger samples are optimal, the relative change in the total cost function is small when the cost of imprecision is a quadratic function.A model‐utility approach, however, may impose a lower bound on sample size that requires the sample size be larger than indicated by the estimation or cost‐minimization approaches. Graphs are provided to illustrate lower‐bound conditions on sample size. Optimal sample size in view of all considerations is obtained by the maximin criterion, the maximum of the minimum sample size for all approaches.