bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discrepancy between IDSA and ESGBOR in Lyme disease: Individual participant meta‐analysis in Türkiye
Authors: Yıldız Abdullah Burak , Çetin Ecesu , Pınarlık Fatihan , Keske Şiran , Can Füsun , Ergönül Önder 
Published: February 2024

AbstractBackgroundThe evidence on the prevalence of Lyme borreliosis (LB) is limited, but there is a suspicion of overdiagnosis of LB in recent years. We reviewed the LB diagnosis and treatment‐related data in Türkiye, based on the Infectious Diseases Society of America (IDSA) 2020 and European Society of Clinical Microbiology and Infectious Diseases Study Group for Lyme Borreliosis (ESGBOR) 2018 guidelines. By detecting the disagreements between these two, we outlined the areas to be improved for future guidelines.MethodsWe performed a literature search according to the PRISMA guidelines in PubMed, Ovid‐Medline, Web of Science, Turkish Medline, Scopus, CINAHL, ULAKBIM TR Index, Google Scholar and Cochrane Library databases. We included the published cases in a database and evaluated according to IDSA and ESGBOR guidelines. We outlined the reasons for misdiagnoses and inappropriate uses of antibiotics.ResultsWe included 42 relevant studies with 84 LB cases reported from Türkiye between 1990 and December 2022. Among 84 cases, the most common clinical findings were nervous system findings (n = 37, 44.0%), erythema migrans (n = 29, 34.5%) and ophthalmologic findings (n = 15, 17.9%). The IDSA 2020 and ESGBOR 2018 guidelines agreed on the diagnosis of 71 (84.5%) cases; there was an agreement that 31 cases (36.9%) were misdiagnosed and 40 cases (47.6%) were correctly diagnosed, and there was disagreement for 13 cases (15.5%). Serum immunoglobulin M (IgM), IgG measurements by ELISA and western blot were widely performed, and they were effective in definitive diagnosis merely when used according to guidelines. Inappropriate use of antibiotics was detected in 42 (50.0%) of cases which were classified in the following categories: incorrect LB diagnosis, inappropriate choice of antibiotic, inappropriate route of drug administration and prolonged antibiotic treatment.ConclusionOverdiagnosis and non‐adherence to guidelines is a common problem. The discordance between seroprevalence and clinical studies necessitates a consensus over the best clinical approach.
Grid Convergence Properties of Wall-Modeled Large Eddy Simulations in the Asymptotic Regime
Authors: Yang Xiang I. A. , Abkar Mahdi , Park George 
Published: February 2024

Abstract
This study explores the grid convergence properties of wall-modeled large eddy simulation (WMLES) solutions as the large eddy simulation (LES) grid approaches the direct numerical simulation (DNS) grid. This aspect of WMLES is fundamental but has not been previously investigated or documented. We investigate two types of grid refinements: one where the LES/wall-model matching location is fixed at an off-wall grid point, and another where the matching location is fixed at a specific distance from the wall. In both cases, we refine the LES grid simultaneously in all three Cartesian directions, with grid resolution ranging from typical LES resolution to typical DNS resolution. Our focus is on examining the mean flow and turbulent kinetic energy (TKE) as the grid refines. While the turbulence statistics consistently converge toward the DNS solution, we observe nonmonotonic convergence in the mean flow statistics. We show that improving the grid resolution does not necessarily enhance the accuracy of the mean flow predictions. Specifically, we identify a negative log layer mismatch when the LES/wall-model matching location lies below the logarithmic layer, regardless of grid resolution and matching location. Finally, we demonstrate that the nonmonotonic convergence of the mean flow can lead to misleading conclusions from grid convergence studies of WMLES.
Effect of modification of penicillin-binding protein 3 on susceptibility to ceftazidime-avibactam, imipenem-relebactam, meropenem-vaborbactam, aztreonam-avibactam, cefepime-taniborbactam, and cefiderocol of
Escherichia coli
strains producing broad-spectrum β-lactamases
Authors: Le Terrier Christophe , Nordmann Patrice , Buchs Chloé , Poirel Laurent 
Published: February 2024

ABSTRACT

The impact of penicillin-binding protein 3 (PBP3) modifications that may be identified in
Escherichia coli
was evaluated with respect to susceptibility to β-lactam/β-lactamase inhibitor combinations including ceftazidime-avibactam, imipenem-relebactam, meropenem-vaborbactam, aztreonam-avibactam, cefepime-taniborbactam, and to cefiderocol. A large series of
E. coli
recombinant strains producing broad-spectrum β-lactamases was evaluated. While imipenem-relebactam showed a similar activity regardless of the PBP3 background, susceptibility to other molecules tested was affected at various levels. This was particularly the case for ceftazidime-avibactam, aztreonam-avibactam, and cefepime-taniborbactam.
An atypical 3-ketoacyl ACP synthase III required for acyl homoserine lactone synthesis in
Pseudomonas syringae
pv.
syringae
B728a
Authors: Guo Qiaoqiao , Su Jingtong , Liao Yuling , Yu Yin , Luo Lizhen , Weng Xiaoshan , Zhang Wenbin , Hu Zhe , Wang Haihong , Beattie Gwyn A. , Ma Jincheng 
Published: February 2024

ABSTRACT

The last step of the initiation phase of fatty acid biosynthesis in most bacteria is catalyzed by the 3-ketoacyl-acyl carrier protein (ACP) synthase III (FabH).
Pseudomonas syringae
pv.
syringae
strain B728a encodes two FabH homologs, Psyr_3467 and Psyr_3830, which we designated PssFabH1 and PssFabH2, respectively. Here, we explored the roles of these two 3-ketoacyl-ACP synthase (KAS) III proteins. We found that PssFabH1 is similar to the
Escherichia coli
FabH in using acetyl-acetyl-coenzyme A (CoA ) as a substrate
in vitro,
whereas PssFabH2 uses acyl-CoAs (C
4
–C
10
) or acyl-ACPs (C
6
–C
10
). Mutant analysis showed that neither KAS III protein is essential for the
de novo
fatty acid synthesis and cell growth. Loss of PssFabH1 reduced the production of an acyl homoserine lactone (AHL) quorum-sensing signal, and this production was partially restored by overexpressing FabH homologs from other bacteria. AHL production was also restored by inhibiting fatty acid elongation and providing exogenous butyric acid. Deletion of PssFabH1 supports the redirection of acyl-ACP toward biosurfactant synthesis, which in turn enhances swarming motility. Our study revealed that PssFabH1 is an atypical KAS III protein that represents a new KAS III clade that functions in providing a critical fatty acid precursor, butyryl-ACP, for AHL synthesis.

IMPORTANCE

Acyl homoserine lactones (AHLs) are important quorum-sensing compounds in Gram-negative bacteria. Although their formation requires acylated acyl carrier proteins (ACPs), how the acylated intermediate is shunted from cellular fatty acid synthesis to AHL synthesis is not known. Here, we provide
in vivo
evidence that
Pseudomonas syringae
strain B728a uses the enzyme PssFabH1 to provide the critical fatty acid precursor butyryl-ACP for AHL synthesis. Loss of PssFabH1 reduces the diversion of butyryl-ACP to AHL, enabling the accumulation of acyl-ACP for synthesis of biosurfactants that contribute to bacterial swarming motility. We report that PssFabH1 and PssFabH2 each encode a 3-ketoacyl-acyl carrier protein synthase (KAS) III in
P. syringae
B728a. Whereas PssFabH2 is able to function in redirecting intermediates from β-oxidation to fatty acid synthesis, PssFabH1 is an atypical KAS III protein that represents a new KAS III clade based on its sequence, non-involvement in cell growth, and novel role in AHL synthesis.
Supplemental Material: The Zaozigou orogenic gold-antimony deposit, West Qinling Orogen, China: Structural controls on multiple mineralization events
Authors: Qiu Kun-Feng , al. et 
Published: February 2024

<p>Table S1: Detailed operating parameters and conditions for dolomite U-Pb dating. Table S2: LA-ICP-MS U-Pb isotope data for dolomite. </p>
Supplemental Material: Recommendations for the reporting and interpretation of isotope dilution U-Pb geochronological information
Authors: Condon Daniel , al. et 
Published: February 2024

<p>Table 1: Example data table for U-Pb CA-ID-TIMS isotopic data for a mineral low in common Pb. Table 2: U-Pb ID-TIMS isotopic data for a single mineral grain high in common Pb. </p>
Thermal Interference When Recording Turbulent Pressure Fluctuations on the Surface of a Floating Device
Authors: Kudashev E. B. , Yablonik L. R. 
Published: February 2024

Abstract
Thermal interference is studied when recording turbulent pressure fluctuations on the surface of a floating device at specified experimental parameters of temperature stratification of an aquatic medium. The effect of distortion of the spectral levels of pressure fluctuations recorded by a sound receiver in the field of temperature inhomogeneities is studied using the example of measurements of turbulent pressure fluctuations in the boundary layer during the vertical ascent of a device from a specified depth. At moderate flow velocities exceeding 1–2 m/s, the temperature susceptibility of a piezoceramic receiver is shown to be decisively determined by its characteristic “thermal” frequency. The parameters of the threshold critical frequency, below which the temperature signal (thermal interference) prevails over the useful signal generated by pressure fluctuations, are determined. With respect to the receivers used in experiments on a floating device [7], the values of the threshold critical frequency are 130 and 215 Hz.
Accelerometer-derived physical activity and the risk of death, heart failure, and stroke in patients with atrial fibrillation: a prospective study from UK Biobank
Authors: Ahn Hyo-Jeong , Choi Eue-Keun , Rhee Tae-Min , Choi JungMin , Lee Kyung-Yeon , Kwon Soonil , Lee So-Ryoung , Oh Seil , Lip Gregory Y H 
Published: February 2024

ObjectiveData on cardiovascular outcomes according to objectively measured physical activity (PA) in patients with atrial fibrillation (AF) are scarce. This study explored the associations between PA derived from wrist-worn accelerometers and the risk of death, incident heart failure (HF), and incident stroke in patients with AF.MethodsFrom 37 990 patients with AF in UK Biobank, 2324 patients with accelerometer data were included. Weekly moderate-to-vigorous PA (MVPA) duration was computed from accelerometer data. The primary outcome was all-cause mortality. Secondary outcomes were cardiovascular mortality, incident HF, and incident stroke. Restricted cubic splines estimated the dose–response associations between MVPA duration and the outcomes. The adjusted HRs (aHRs) of the outcomes according to adherence to PA standard guidelines (performing MVPA≥150 min/week) were also evaluated.ResultsThe mean age was 66.9±6.2 years and 64.9% were male. During a median follow-up of 6.7 years, there were 181 all-cause deaths, 62 cardiovascular deaths, 225 cases of incident HF, and 91 cases of incident stroke; the overall incidence rate per 1000 patient-years was 11.76, 4.03, 15.16 and 5.99, respectively. There was a linear inverse dose–response relationship between MVPA (≥108 min/week) and all-cause mortality. Performing MVPA for 105–590 min/week was associated with a lower risk of HF than those with no measurable MVPA. The risk of stroke and cardiovascular mortality was not associated with MVPA. Performing guideline-adherent MVPA was related to a 30% lower risk of all-cause mortality (aHR: 0.70 (0.50–0.98), p=0.04) and 33% lower risk of HF (aHR 0.67 (0.49–0.93), p=0.02).ConclusionIn patients with AF, accelerometer-derived PA data supports lower risks of all-cause mortality and HF according to a greater level of MVPA and adherence to PA guidelines. Regular MVPA should be encouraged in patients with AF as a part of integrated management.
Occupation-specific risk estimates for suicide and non-fatal self-harm from a Swedish cohort of male construction workers followed 1987–2018
Authors: Mehlig Kirsten , Torén Kjell , LaMontagne Anthony D , Wahlström Viktoria , Nyberg Jenny , Waern Margda , Åberg Maria 
Published: February 2024

ObjectivesWhile suicidal behaviour has become less prevalent in non-manual workers in recent decades, rates have increased in manual workers. We aimed to identify occupations within the construction industry with excess risk of suicide and non-fatal self-harm.MethodsThis cohort of Swedish construction workers comprises 389 132 individuals examined 1971–1993 and followed 1987–2018 using national hospital and cause of death registers. More than 200 job titles were merged into 22 occupational groups. For 296 891 men alive in 1987 and active in the construction sector, survival was calculated from baseline to first event of non-fatal self-harm or suicide and censored for emigration, long-term unemployment, disability pension, retirement, death from other causes or end of follow-up. HRs with 95% CIs were obtained from multiple Cox proportional hazard regression.ResultsOverall, 1618 cases of suicide and 4774 events of non-fatal self-harm were registered. Self-harm before baseline was the single largest risk factor for suicide, HR 9.3 (95% CI 7.5 to 11.6). Compared with the overall mean, labourers and rock workers had excess risk for suicide, HR 1.4 (95% CI 1.1 to 1.7) and 1.5 (95% CI 1.0 to 2.3), respectively, while electricians, clerks and foremen had reduced risk. Labourers, concrete workers, sheet metal workers, painters, glaziers and the group ‘other construction workers’ were at increased risk for non-fatal self-harm. Almost all categories of manual workers were at increased risk for suicidal behaviour relative to clerks and foremen.ConclusionsSpecific occupations within the construction sector were associated with excess risk for suicidal behaviour. Future studies should identify underlying risk factors to inform tailored interventions.
Monocyte NLRP3 inflammasome and interleukin-1β activation modulated by alpha-1 antitrypsin therapy in deficient individuals
Authors: Gogoi Debananda , Yu Howard , Casey Michelle , Baird Rory , Yusuf Azeez , Forde Luke , O' Brien Michael E , West Jesse R , Flagg Tammy , McElvaney Noel G , Eden Edward , Mueller Christian , Brantly Mark L , Geraghty Patrick , Reeves Emer P 
Published: February 2024

IntroductionAltered complement component 3 (C3) activation in patients with alpha-1 antitrypsin (AAT) deficiency (AATD) has been reported. To understand the potential impact on course of inflammation, the aim of this study was to investigate whether C3d, a cleavage-product of C3, triggers interleukin (IL)-1β secretion via activation of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome. The objective was to explore the effect of AAT augmentation therapy in patients with AATD on the C3d/complement receptor 3 (CR3) signalling axis of monocytes and on circulating pro-inflammatory markers.MethodsInflammatory mediators were detected in blood from patients with AATD (n=28) and patients with AATD receiving augmentation therapy (n=19). Inflammasome activation and IL-1β secretion were measured in monocytes of patients with AATD, and following C3d stimulation in the presence or absence of CR3 or NLRP3 inhibitors.ResultsC3d acting via CR3 induces NLRP3 and pro-IL-1β production, and through induction of endoplasmic reticulum (ER) stress and calcium flux, triggers caspase-1 activation and IL-1β secretion. Treatment of individuals with AATD with AAT therapy results in decreased plasma levels of C3d (3.0±1.2 µg/mL vs 1.3±0.5 µg/mL respectively, p<0.0001) and IL-1β (115.4±30 pg/mL vs 73.3±20 pg/mL, respectively, p<0.0001), with a 2.0-fold decrease in monocyte NLRP3 protein expression (p=0.0303), despite continued ER stress activation.DiscussionThese results provide strong insight into the mechanism of complement-driven inflammation associated with AATD. Although the described variance in C3d and NLRP3 activation decreased post AAT augmentation therapy, results demonstrate persistent C3d and monocyte ER stress, with implications for new therapeutics and clinical practice.
Mutant U2AF1-Induced Mis-Splicing of mRNA Translation Genes Confers Resistance to Chemotherapy in Acute Myeloid Leukemia
Authors: Jin Peng , Wang Xiaoling , Jin Qiqi , Zhang Yi , Shen Jie , Jiang Ge , Zhu Hongming , Zhao Ming , Wang Dan , Li Zeyi , Zhou Yan , Li Wenzhu , Zhang Wei , Liu Yabin , Wang Siyang , Jin Wen , Cao Yuncan , Sheng Guangying , Dong Fangyi , Wu Shishuang , Li Xiaoyang , Jin Zhen , He Mengke , Liu Xiaxin , Chen Luonan , Zhang Yunxiang , Wang Kankan , Li Junmin 
Published: February 2024

Abstract

Patients with primary refractory acute myeloid leukemia (AML) have a dismal long-term prognosis. Elucidating the resistance mechanisms to induction chemotherapy could help identify strategies to improve AML patient outcomes. Herein, we retrospectively analyzed the multiomics data of more than 1,500 AML cases and found that patients with spliceosome mutations had a higher risk of developing refractory disease. RNA splicing analysis revealed that the mis-spliced genes in refractory patients converged on translation-associated pathways, promoted mainly by U2AF1 mutations. Integrative analyses of binding and splicing in AML cell lines substantiated that the splicing perturbations of mRNA translation genes originated from both the loss and gain of mutant U2AF1 binding. In particular, the U2AF1S34F and U2AF1Q157R mutants orchestrated the inclusion of exon 11 (encoding a premature termination codon) in the eukaryotic translation initiation factor 4A2 (EIF4A2). This aberrant inclusion led to reduced eIF4A2 protein expression via nonsense-mediated mRNA decay. Consequently, U2AF1 mutations caused a net decrease in global mRNA translation that induced the integrated stress response (ISR) in AML cells, which was confirmed by single-cell RNA sequencing. The induction of ISR enhanced the ability of AML cells to respond and adapt to stress, contributing to chemoresistance. A pharmacologic inhibitor of ISR, ISRIB, sensitized U2AF1 mutant cells to chemotherapy. These findings highlight a resistance mechanism by which U2AF1 mutations drive chemoresistance and provide a therapeutic approach for AML through targeting the ISR pathway.

Significance:
U2AF1 mutations induce the integrated stress response by disrupting splicing of mRNA translation genes that improves AML cell fitness to enable resistance to chemotherapy, which can be targeted to improve AML treatment.
ATPase Copper Transporting Beta (ATP7B) Is a Novel Target for Improving the Therapeutic Efficacy of Docetaxel by Disulfiram/Copper in Human Prostate Cancer
Authors: Song Liankun , Nguyen Vyvyan , Xie Jun , Jia Shang , Chang Christopher J. , Uchio Edward , Zi Xiaolin 
Published: February 2024

Abstract
Docetaxel has been the standard first-line chemotherapy for lethal metastatic prostate cancer (mPCa) since 2004, but resistance to docetaxel treatment is common. The molecular mechanisms of docetaxel resistance remain largely unknown and could be amenable to interventions that mitigate resistance. We have recently discovered that several docetaxel-resistant mPCa cell lines exhibit lower uptake of cellular copper and uniquely express higher levels of a copper exporter protein ATP7B. Knockdown of ATP7B by silencing RNAs (siRNA) sensitized docetaxel-resistant mPCa cells to the growth-inhibitory and apoptotic effects of docetaxel. Importantly, deletions of ATP7B in human mPCa tissues predict significantly better survival of patients after their first chemotherapy than those with wild-type ATP7B (P = 0.0006). In addition, disulfiram (DSF), an FDA-approved drug for the treatment of alcohol dependence, in combination with copper, significantly enhanced the in vivo antitumor effects of docetaxel in a docetaxel-resistant xenograft tumor model. Our analyses also revealed that DSF and copper engaged with ATP7B to decrease protein levels of COMM domain–containing protein 1 (COMMD1), S-phase kinase–associated protein 2 (Skp2), and clusterin and markedly increase protein expression of cyclin-dependent kinase inhibitor 1 (p21/WAF1). Taken together, our results indicate a copper-dependent nutrient vulnerability through ATP7B exporter in docetaxel-resistant prostate cancer for improving the therapeutic efficacy of docetaxel.
Aus Europa nach Greifswald
Authors: Bamberger Gudrun 
Published: February 2024

Abstract
This article deals with Sibylla Schwarz’ poetic confrontation with the literary authority of the early 17th century par excellence. In Martin Opitz, she not only found a model to follow, but also a point of orientation for her own approach to writing, which is why she productively transforms his material and texts, but develops her own forms and arguments in this imitation. She repeatedly addresses him explicitly as an ally in matters of poetry and the implementation of poetic guidelines, who, like herself, had to overcome resistance. Both his texts and his personality are appreciated in this context and integrated into her own writing. In doing so, she follows Opitz in a strategy propagated by himself and thus continues it.
“Was Sappho für ein Weib gewesen …”
Authors: Philips Samantha 
Published: February 2024

Abstract
In this article the author analyses the characterization of Sibylla Schwarz as “pommersche Sappho”. The aim is to carve out basic motivic structures as well as peculiarities of a Sapphic persona in poetry, on which Sibylla Schwarz was able to draw on in her writing and to which she is determined by her contemporaries and recipients when she is established in the literary world via the honorary title “Sappho”. In doing so, it is shown that sapphic writing is bound to certain expectations, such as musicality and high quality of the poems, reflection on strong emotions, a – partly concealed – inclusion of female homoerotic undertones, and the thematisation of the possibility of being driven to death by the power of passion as well as the unbearable bittersweetness of love. In her poems Schwarz shows her knowledge of Sappho as a female role model for women writers, but does not define her poet persona primarily or emphatically in the succession of the Greek lyric poetess Sappho.
Hegel’s Criticism of Pyrrhonism
Authors: Spigt Joris 
Published: February 2024

Abstract
This paper presents Hegel’s criticism of two central ideas of Pyrrhonism: the importance of stating only how things appear and Pyrrhonism as a way of life. After providing a sketch of the main features of Pyrrhonism, the paper lays out and critically evaluates Hegel’s largely unexamined argument against Pyrrhonism in his early 1802 essay on skepticism. Hegel claims that the Pyrrhonist’s appeal to appearance renders Pyrrhonism philosophically vacuous: insofar as Pyrrhonism merely describes the subjective contents of the Pyrrhonist’s mind, it has no philosophical import. Sextus would not accept Hegel’s criticism because the appeal to appearance could express the provisional rather than purely subjective character of Pyrrhonism. The paper proceeds by examining Hegel’s argument in the Phenomenology that skepticism is contradictory on account of conjoining suspension of judgment with acquiescing in appearances as the guide to life. Sextus would reject Hegel’s criticism by insisting on the distinction between judgment and appearance.