bluuebunny/crossref_metadata_2025_split huggingface dataset:
Secondary flow due to Alfvén waves
Authors: Shercliff J. A. 
Published: April 2006

Large (gigajoule) amounts of energy can in principle be stored as kinetic energy in liquid metal circulating round a torus and can be extracted at the gigawatt level by Alfvén waves propagating along an imposed axial field. A major limitation on the energy that may be so stored is the disruption of these primary Alfvén waves by secondary flows in meridional planes, associated with out-of-balance centrifugal forces ahead of and behind the waves and non-uniform magnetic pressures at the wave fronts. Vorticity, created at the wave, itself propagates in secondary Alfvén waves.This paper gives a linearized treatment of these secondary motions and the associated perturbations of the imposed axial field and compares the resulting disruption of the primary wave mode with crude estimates made in an earlier paper. The main case treated is the discharge of the stored energy into a matched resistor by an Alfvén step wave but the secondary consequences of standing primary waves are also explored. The nature of the solutions depends on the electromagnetic characteristics of the walls normal to the imposed field. The problem is mathematically interesting because it involves the joint solving of elliptic and hyperbolic equations that are coupled by the boundary conditions at these walls.
Cavitation noise and inception as influenced by boundary-layer development on a hydrofoil
Authors: Blake William K. , Wolpert M. J. , Geib F. E. 
Published: April 2006

This paper describes measurements of noise from two-phase flow over hydrofoils. The experiments were performed in a variable-pressure water tunnel which was acoustically calibrated so that sound power levels could be deduced from the sound measurements. It is partially reverberant in the frequency range of interest.Cavitation was generated on a hydrofoil in the presence of either a separated laminar boundary layer or a fully turbulent attached boundary layer. The turbulent boundary layer was formed downstream of a trip which was positioned near the leading edge. High-speed photographs show the patterns of cavitation which were obtained in each case. The noise is shown to depend on the type of cavitation produced; and for each type, the dependence on speed and cavitation index has been determined. Dimensionless spectral densities of the sound are shown for each type of flow.
Cavitation in lubrication. Part 2. Analysis of wavy interfaces
Authors: Savage M. D. 
Published: April 2006

The steady and unifrom flow of a viscous fluid past a unifrom cavity in a gemoetry with small, yet arbitrary, film thickness is considered. A mathematical model for describing steady perturbations to such a flow is presented in which the perturbation to the cavity-fluid interface is represented by a small amplitude harmonic wave of wavenumber n. A linearized perturbation analysis then permits the formulation of a boundary-value problem involving the homogeneous Reynolds equation, the solution to which determines both n and the perturbed pressure field.Numerical and approximate analytic solutions are determined for the cylinderplane geometry in which fluid flows between a rotating cylinder and a Perspex block. Whilst these compare well with experimental data over the whole range
\[
0.1 < \eta U/T < 3,
\]
closest agreement between theory and experiment is attained for small values of both ηU/T and n.
Relationship between tumour-node-metastasis stage of laryngeal squamous cell carcinoma and duration of symptoms
Authors: Özbilen S , Aydil U , Dinç A E , Kizil Y , Köybaşioğlu A 
Published: April 2006

Objective: To evaluate the relationship between disease stage and duration of symptoms for squamous cell carcinoma of the larynx.Method: We evaluated retrospectively data from 102 laryngeal squamous cell carcinoma patients seen in the otorhinolaryngology department of the Gazi University School of Medicine between December 1996 and June 2004. Inclusion criteria were a histologic diagnosis of laryngeal squamous cell carcinoma, no previous medical, surgical or radiological treatment for carcinoma, and the absence of any other primary tumours or distant metastatic disease.Results: There was no relationship between duration of symptoms and stage of the disease, both for glottic and supraglottic tumours. When glottic and supraglottic tumours were compared, no significant difference in symptom duration could be found.Conclusion: There is no correlation between symptom duration and the stage of the disease for squamous cell carcinoma of the larynx. Therefore, symptom duration does not reflect the stage of the tumour.
MITF mediates cAMP-induced protein kinase C-β expression in human melanocytes
Authors: Park Hee-Young , Wu Christina , Yonemoto Laurie , Murphy-Smith Melissa , Wu Heng , Stachur Christina M. , Gilchrest Barbara A. 
Published: April 2006

The cAMP-dependent pathway up-regulates MITF (microphthalmia-associated transcription factor), important for key melanogenic proteins such as tyrosinase, TRP-1 (tyrosinase-related protein 1) and TRP-2. We asked whether MITF is also a key transcription factor for PKC-β (protein kinase C-β), required to phosphorylate otherwise inactive tyrosinase. When paired cultures of human melanocytes were treated with isobutylmethylxanthine, known to increase intracellular cAMP, both protein and mRNA levels of PKC-β were induced by 24 h. To determine whether MITF modulates PKC-β expression, paired cultures of human melanocytes were transfected with dn-MITF (dominant-negative MITF) or empty control vector. By immunoblotting, PKC-β protein was reduced by 63±3.7% within 48 h. Co-transfection of an expression vector for MITF-M, the MITF isoform specific for pigment cells, or empty control vector with a full-length PKC-β promoter–CAT (chloramphenicol acetyltransferase) reporter construct (PKC-β/CAT) into Cos-7 cells showed &gt;60-fold increase in CAT activity. Melanocytes abundantly also expressed MITF-A, as well as the MITF-B and MITF-H isoforms. However, in contrast with MITF-M, MITF-A failed to transactivate co-expressed PKC-β/CAT or CAT constructs under the control of a full-length tyrosinase promoter. Together, these results demonstrate that MITF, specifically MITF-M, is a key transcription factor for PKC-β, linking the PKC- and cAMP-dependent pathways in regulation of melanogenesis.
Role of Nrf2 in the regulation of the Mrp2 (ABCC2) gene
Authors: Vollrath Valeska , Wielandt Ana M. , Iruretagoyena Mirentxu , Chianale Jose 
Published: April 2006

The Nrf2 (nuclear factor-erythroid 2 p45-related factor 2) transcription factor regulates gene expression of the GCLC (glutamate–cysteine ligase catalytic subunit), which is a key enzyme in glutathione synthesis, and GSTs (glutathione S-transferases) via the ARE (antioxidant-response element). The Mrp2 (multidrug-resistance protein 2) pump mediates the excretion of GSH and GSSG excretion as well as endo- and xeno-biotics that are conjugated with GSH, glucuronate or sulphate. Considering that Mrp2 acts synergistically with these enzymes, we hypothesized that the regulation of Mrp2 gene expression is also dependent on Nrf2. Using BHA (butylated hydroxyanisole), which is a classical activator of the ARE–Nrf2 pathway, we observed an increase in the transcriptional activity of Mrp2, GCLC and Gsta1/Gsta2 genes in the mouse liver. A similar pattern of co-induction of Mrp2 and GCLC genes was also observed in mouse (Hepa 1-6) and human (HepG2) hepatoma cells treated with BHA, β-NF (β-naphthoflavone), 2,4,5-T (trichlorophenoxyacetic acid) or 2AAF (2-acetylaminofluorene), suggesting that these genes share common mechanism(s) of transcriptional activation in response to exposure to xenobiotics. To define the mechanism of Mrp2 gene induction, the 5′-flanking region of the mouse Mrp2 gene (2.0 kb) was isolated, and two ARE-like sequences were found: ARE-2 (−1391 to −1381) and ARE-1 (−95 to −85). Deletion analyses demonstrated that the proximal region (−185 to +99) contains the elements for the basal expression and xenobiotic-mediated induction of the Mrp2 gene. Gel-shift and supershift assays indicated that Nrf2–protein complexes bind ARE sequences of the Mrp2 promoter, preferentially to the ARE-1 sequence. Overexpression of Nrf2 increased ARE-1-mediated CAT (chloramphenicol acetyltransferase) gene activity, while overexpression of mutant Nrf2 protein repressed the activity. Thus Nrf2 appears to regulate Mrp2 gene expression via an ARE element located at the proximal region of its promoter in response to exposure to xenobiotics.
Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer–micelle association
Authors: Hughes Richard K. , Belfield Eric J. , Muthusamay Mylrajan , Khan Anuja , Rowe Arthur , Harding Stephen E. , Fairhurst Shirley A. , Bornemann Stephen , Ashton Ruth , Thorneley Roger N. F. , Casey Rod 
Published: April 2006

We describe the detailed biochemical characterization of CYP74C3 (cytochrome P450 subfamily 74C3), a recombinant plant cytochrome P450 enzyme with HPL (hydroperoxide lyase) activity from Medicago truncatula (barrel medic). Steady-state kinetic parameters, substrate and product specificities, RZ (Reinheitszahl or purity index), molar absorption coefficient, haem content, and new ligands for an HPL are reported. We show on the basis of gel filtration, sedimentation velocity (sedimentation coefficient distribution) and sedimentation equilibrium (molecular mass) analyses that CYP74C3 has low enzyme activity as a detergent-free, water-soluble, monomer. The enzyme activity can be completely restored by re-activation with detergent micelles, but not detergent monomers. Corresponding changes in the spin state equilibrium, and probably co-ordination of the haem iron, are novel for cytochrome P450 enzymes and suggest that detergent micelles have a subtle effect on protein conformation, rather than substrate presentation, which is sufficient to improve substrate binding and catalytic-centre activity by an order of magnitude. The kcat/Km of up to 1.6×108 M−1·s−1 is among the highest recorded, which is remarkable for an enzyme whose reaction mechanism involves the scission of a C–C bond. We carried out both kinetic and biophysical studies to demonstrate that this effect is a result of the formation of a complex between a protein monomer and a single detergent micelle. Association with a detergent micelle rather than oligomeric state represents a new mechanism of activation for membrane-associated cytochrome P450 enzymes. Highly concentrated and monodispersed samples of detergent-free CYP74C3 protein may be well suited for the purposes of crystallization and structural resolution of the first plant cytochrome P450 enzyme.
Structure and stability of 2S albumin-type peanut allergens: implications for the severity of peanut allergic reactions
Authors: Lehmann Katrin , Schweimer Kristian , Reese Gerald , Randow Stefanie , Suhr Martin , Becker Wolf-Meinhard , Vieths Stefan , Rösch Paul 
Published: April 2006

Resistance to proteolytic enzymes and heat is thought to be a prerequisite property of food allergens. Allergens from peanut (Arachis hypogaea) are the most frequent cause of fatal food allergic reactions. The allergenic 2S albumin Ara h 2 and the homologous minor allergen Ara h 6 were studied at the molecular level with regard to allergenic potency of native and protease-treated allergen. A high-resolution solution structure of the protease-resistant core of Ara h 6 was determined by NMR spectroscopy, and homology modelling was applied to generate an Ara h 2 structure. Ara h 2 appeared to be the more potent allergen, even though the two peanut allergens share substantial cross-reactivity. Both allergens contain cores that are highly resistant to proteolytic digestion and to temperatures of up to 100 °C. Even though IgE antibody-binding capacity was reduced by protease treatment, the mediator release from a functional equivalent of a mast cell or basophil, the humanized RBL (rat basophilic leukaemia) cell, demonstrated that this reduction in IgE antibody-binding capacity does not necessarily translate into reduced allergenic potency. Native Ara h 2 and Ara h 6 have virtually identical allergenic potency as compared with the allergens that were treated with digestive enzymes. The folds of the allergenic cores are virtually identical with each other and with the fold of the corresponding regions in the undigested proteins. The extreme immunological stability of the core structures of Ara h 2 and Ara h 6 provides an explanation for the persistence of the allergenic potency even after food processing.
A quantitative lymphoscintigraphic evaluation of lymphatic function in the swollen hands of women with lymphoedema following breast cancer treatment
Authors: Stanton Anthony W. B. , Modi Stephanie , Mellor Russell H. , Peters A. Mike , Svensson William E. , Levick J. Rodney , Mortimer Peter S. 
Published: April 2006

In BCRL (breast cancer-related lymphoedema), arm swelling is unevenly distributed and some regions are partly or entirely spared. In particular, the hand may or not be swollen, but when involved functional impairment can be substantial. We have found previously that, when the ipsilateral hand is spared of swelling (in a limb with swelling proximal to the hand), the local lymph drainage rate constant (k) is at least as high as in the contralateral hand, contrary to the traditional ‘stopcock’ concept of reduced lymph drainage from the whole limb. In the light of this finding, we have investigated lymph drainage in the hands of eight women with BCRL and moderate-to-severe hand swelling, using γ-camera quantitative lymphoscintigraphy. Images showed pronounced superficial activity in the ipsilateral swollen arms of most patients, indicating dermal backflow. k for 99mTc-labelled hIgG (human IgG) measured over 5 h in the subcutis of the ipsilateral swollen hand was 34±24% less than in the contralateral hand (P=0.013). Activity measured in the ipsilateral swollen forearm increased progressively, but there was very little increase in the contralateral forearm, indicating retention of 99mTc-labelled hIgG in the swollen forearm. It is concluded that lymphatic function in the swollen hand is impaired, and that there appears to be two populations of women with BCRL, i.e. spared-hand and swollen-hand, irrespective of the cancer treatment received.
The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term
Authors: Bates David O. , MacMillan Philip P. , Manjaly Joseph G. , Qiu Yan , Hudson Sarah J. , Bevan Heather S. , Hunter Alyson J. , Soothill Peter W. , Read Michael , Donaldson Lucy F. , Harper Steven J. 
Published: April 2006

PET (pre-eclamptic toxaemia) has recently been linked with alterations in production of a VEGFR1 [VEGF (vascular endothelial growth factor) receptor 1] splice variant that acts as a circulating inhibitor. We have recently described a family of naturally occurring splice variants of VEGF, termed VEGFxxxb, that also appear to act as inhibitors of conventional VEGFxxx-mediated angiogenesis. To determine whether alteration in splicing of VEGF–VEGFR family members extended beyond VEGFR1, we investigated the effect of pre-eclampsia on placental VEGFxxxb mRNA and protein expression. VEGFxxx and VEGFxxxb mRNA and protein were both found in normal human term placentae. VEGFxxx protein formed the majority of the total VEGF protein (980±195 pg/mg), whereas VEGFxxxb (11.5 pg/mg) was found to form a small part of the total VEGF protein expression (1.5±0.24%). Evidence for VEGF165b, VEGF121b and VEGF145b expression was found. In pre-eclamptic placentae, there was a significant down-regulation of VEGFxxxb isoforms, but a small up-regulation of VEGFxxx isoforms. In normal placenta VEGFxxxb and VEGFxxx concentrations were positively correlated (r=0.69, P&lt;0.02), whereas in pre-eclamptic placentae, there was a significant negative correlation between VEGFxxxb and VEGFxxx protein expression (r=−0.8, P&lt;0.02), indicating that there was a significant uncoupling of the splicing regulation of the VEGF isoforms. Combined with previous studies showing increased soluble VEGFR1 isoforms in human pre-eclampsia, these data suggest that there may be a common mechanism in pre-eclampsia that involves dysregulation of mRNA splicing of members of the VEGF–VEGFR axis.
ArrayCluster: an analytic tool for clustering, data visualization and module finder on gene expression profiles
Authors: Yoshida Ryo , Higuchi Tomoyuki , Imoto Seiya , Miyano Satoru 
Published: April 2006

Abstract
Summary: One of the significant challenges in gene expression analysis is to find unknown subtypes of several diseases at the molecular levels. This task can be addressed by grouping gene expression patterns of the collected samples on the basis of a large number of genes. Application of commonly used clustering methods to such a dataset however are likely to fail owing to over-learning, because the number of samples to be grouped is much smaller than the data dimension which is equal to the number of genes involved in the dataset. To overcome such difficulty, we developed a novel model-based clustering method, referred to as the mixed factors analysis. The ArrayCluster is a freely available software to perform the mixed factors analysis. It provides us some analytic tools for clustering DNA microarray experiments, data visualization and an automatic detector for module transcriptional of genes that are relevant to the calibrated molecular subtypes and so on.
Availability: The ArrayCluster can be used free of charge for non-commercial and academic use and downloaded from
Contact: yoshidar@ims.u-tokyo.ac.jp
Protective role of supplement with foreign Bifidobacterium and Lactobacillus in experimental hepatic ischemia‐reperfusion injury
Authors: Xing Hui‐Chun , Li Lan‐Juan , Xu Kai‐Jin , Shen Tian , Chen Yun‐Bo , Sheng Ji‐Fang , Chen Yu , Fu Su‐Zhen , Chen Chun‐Lei , Wang Jian‐Guo , Yan Dong , Dai Fang‐Wei , Zheng Shu‐Sen 
Published: April 2006

AbstractBackground and Aim:  Intestinal microflora play a crucial role in some severe liver diseases. The purpose of this study was to evaluate the effects of a Lactobacillus strain and a Bifidobacterium strain on ischemia‐reperfusion (I/R) liver injury.Methods:  Rats were divided into six groups. Each group received either Bifidobacterium Catenulatum ZYB0401; Lactobacillus Fermentum ZYL0401; a mixture of these two bacterial strains; gentamicin; or saline by daily gavage for 7 days. On the sixth day, all rats, except those in the control group, were subjected to 20 min of liver ischemia. After 22 h of hepatic reperfusion, liver enzymes and histology, malondialdehyde (MDA), superoxide dismutase (SOD), endotoxemia, serum tumor necrosis factor‐α (TNF‐α), intestinal bacteria, intestinal mucosal ultrastructure, and bacterial translocation were studied.Results:  All administered bacteria increased intestinal Bifidobacterium and Lactobacillus, decreased endotoxemia (P < 0.01), alanine aminotransferase (ALT) (P < 0.01), and markedly ameliorated liver histology and intestinal mucosal ultrastructure. Only rats treated with Bifidobacterium Catenulatum ZYB0401 and Lactobacillus Fermentum ZYL0401 showed reduced incidence of bacterial translocation to the kidney (P < 0.05), associated with decreased serum TNF‐α and liver MDA (P < 0.05) and increased liver SOD (P < 0.05) compared to the I/R group. Gentamicin decreased almost all kinds of intestinal bacteria (P < 0.01) and decreased ALT (P < 0.01) and serum TNF‐α, but failed to reduce both endotoxemia and the incidence of bacterial translocation and had no effects on liver MDA and SOD.Conclusion:  Bifidobacterium Catenulatum ZYB0401 in combination with Lactobacillus Fermentum ZYL0401 could be useful in restoring intestinal microflora and in preventing liver injury in hepatic I/R of rats.
Extranodal conjunctival Hodgkin's‐like lymphoma in a cat
Authors: Holt Elaine , Goldschmidt Michael H. , Skorupski Katherine 
Published: April 2006

AbstractA seven‐year‐old female spayed Domestic Short‐haired cat was brought for examination following a four‐month history of conjunctivitis in the right eye. The superior palpebral conjunctiva was diffusely swollen and firm, displacing the right globe ventrally and preventing full closure of the eyelids. The right popliteal lymph node was enlarged as were several other peripheral lymph nodes. An incisional biopsy of the conjunctival swelling revealed a lymphoid neoplasm, the cellular make up of which suggested features of feline Hodgkin's‐like lymphoma. Although the neoplasm was poorly responsive to standard chemotherapeutic protocols for lymphoma, radiation therapy rapidly reduced its size. Three years following the initial diagnosis, there has been no recurrence of the conjunctival neoplasm. To the authors’ knowledge, there are no previous reports of extranodal or adnexal (conjunctiva, eyelid, orbit) Hodgkin's‐like lymphoma in the veterinary literature.
Calcification associated with the nasolacrimal system of a horse: case report and mineralogic composition
Authors: Cassotis Nicholas J. , Schiffman Peter 
Published: April 2006

AbstractA 12‐year‐old Paso Fino mare was evaluated for a chronic nasal and ocular discharge. A calcification was identified eroding through the nasal mucosa of the middle meatus. Differential considerations for this mass included dacryolith, nasal calculus, or calcified tooth root abscess. Anatomical location and case history and progression supported a dacryolith. Following removal, the calculus was submitted for mineralogic analysis. The calculus was primarily carbonate hydroxylapatite (Ca10(PO4)3(CO3)3(OH)2) with a minor NaCl halite constituent. Dacryoliths, or nasolacrimal calculi, are an uncommon occurrence in animals, and sparsely reported in humans. The etiopathogenesis of dacryolith formation is unknown. The objective of this article was to report the first case report of an equine nasolacrimal system dacryolith and its mineralogic composition.
Interspecies variation in orbital bone structure of psittaciform birds (with emphasis on Psittacidae)
Authors: Machado Marcello , Dos Santos Schmidt Elizabeth Moreira , Montiani‐Ferreira Fabiano 
Published: April 2006

AbstractObjective  This text presents an anatomical study of the normal bony orbital structure of a sample of different bird species belonging to the order Psittaciformes.Procedures  The bony anatomy of Psittaciformes’ skulls was examined and described using cadavers of birds that were presented already dead to the Federal University of Paraná, Brazil or had been euthanized for humane reasons. Dissections of the orbital cavity were performed under 2–4 × magnification, and descriptions of the orbital bones were made from observations of macerated skulls that had been boiled and cleaned. The present paper discusses the main features of the bony orbit of psittaciform birds, describing known anatomical information but also bringing new information, mainly concerning species differences that might help not only veterinary anatomists but also zoologists, clinicians, researchers, and students of veterinary ophthalmology to better comprehend this order of birds.Results and conclusions  Variations in the anatomic conformation of the bony elements of the orbit were observed in different species of Psittaciformes. Based on these differences, Psittaciformes were classified into two different groups. The first group of Psittaciformes shows an enclosed (complete) bony orbit formed by the junction of the orbital with the postorbital processes, creating a suborbital arch. The second group of Psittaciformes essentially lacked a suborbital arch, presenting an open (incomplete) bony orbit, typical of most modern birds. In the latter group, orbital and postorbital processes are present.