bluuebunny/crossref_metadata_2025_split huggingface dataset:
HUBUNGAN HYGIENE SANITASI PEDAGANG SOSIS DENGAN ANGKA KUMAN PADA SOSIS BAKAR YANG DIPERDAGANGKAN DI JALAN DAYA NASIONAL PONTIANAK TENGGARA
Authors: Nadia Nadia , Paulina Paulina , Suharno Suharno 
Published: March 2024

Makanan jajanan dipinggir jalan umumnya mudah terkontaminasi oleh kuman. Tujuan penelitian menganalisis Hubungan Hygiene Sanitasi Pedagang Sosis Terhadap Angka Kuman Pada Sosis Bakar Di Jalan Daya Nasional Pontianak Tenggara. Metode yang digunakan adalah Analitik Observasional. Populasi penelitian 30 pedagang sosis bakar. Hasil penelitian menunjukan sumber sosis baik 17 (56,7%), personal hygiene pedagang, hygiene sanitasi peralatan dan sanitasi tempat penjualan tidak baik 21 (70%), 27 (90%), 28 (93,3%), angka kuman pada sosis bakar tidak memenuhi syarat 19 sampel (63,3%). Kesimpulan penelitian adalah ada hubungan antara sumber sosis dan personal hygiene pedagang dengan angka kuman pada sosis bakar dengan masing-masing p-value = 0,040 dan p-value = 0,004, serta tidak ada hubungan antara hygiene sanitasi peralatan dan sanitasi tempat penjualan dengan angka kuman pada sosis bakar p-value = 0,298 dan p-value = 0,607. Diharapkan para pedagang menerapakan personal hygiene serta dapat memilih tempat jajanan yang sehat.
Nomenclatural standards of black currant cultivars bred by the I.V. Michurin Federal Scientific Center
Authors: Talovina G. V. , Zhidekhina T. V. , Dunaeva S. E. , Gur’eva I. V. , Rodyukova O. S. , Klimenko N. S. , Kuzmina E. V. , Gavrilenko T. A. 
Published: March 2024

According to the recently proposed comprehensive strategy for the registration and conserving of Russian varieties of vegetatively propagated crops in the VIR genebank, plant material is transferred by cultivar authors to VIR to create nomenclatural standards and preserve them in the VIR Herbarium of Cultivated Plants, their Wild Relatives and Weeds (WIR), as well as for viable preservation in the VIR in vitro, cryo- and field collections and for genotyping the cultivars. Plant material of 14 of black currant cultivars was collected by colleagues from the I.V. Michurin Federal Scientific Center (I.V. Michurin FSC), including the cultivar authors, and transferred to the VIR Herbarium for preparing of nomenclatural standards. In accordance with the rules of the International Code of Nomenclature of Cultivated Plants, nomenclatural standards have been prepared for the following black currant cultivars: ‘Amirani’, ‘Bagira’, ‘Vospominanie’, ‘Zelënai͡a Dymka’, ‘Malenʹkiĭ Print͡s’, ‘Pami󠆎͡atʹ Michurina’, ‘Sozvezdie’, ‘Tamerlan’, ‘Tatʹi͡anin Denʹ’, ‘Charovnit͡sa’, ‘Chernavka’, ‘Chërnyĭ Zhemchug’, ‘Shalunʹi͡a’, ‘Ėlevesta’. Nomenclature standards registered in the VIR Herbarium database were transferred for storage to the VIR Herbarium of cultivated plants of the world, their wild relatives and weeds (WIR). Plant material transferred from the I.V. Michurin FSC was used not only for herbarization and creation of nomenclatural standards, but also for live preservation of cultivars in the VIR in vitro and field collections.
The Effect of NAA, BAP and NAA, BAP Combinations on the Growth of In-vitro Culturally Promoted Leaves of Sansevieria ehrenbergii
Authors: Fatikha Ajeng Safitr  , Tengku Nurhidayah  
Published: March 2024

Sansevieria is an ornamental succulent leaf plant of the family Agavaceae, having a beautiful and diverse shape and color. Propagation of Sansevieria can be done by tissue culture method with the addition of cytokinins and auxins to MS media. The study used a complete randomized design consisting of 8 treatments, 3 tests, there were 24 experimental units. The observed parameters are when the callus appears, the color of the callus, the texture of the callus, the weight of the callus, the diameter of the callus, and the percentage of success in growing. Data analysis used variance analysis and tested using 5% DNMRT. The data is processed using a Statistical Analysis System (SAS). The results showed that the application of NAA, BAP, and the combination of NAA and BAP affected the per-plant explant of Sansevieria ehrenbergii leaves on all observation parameters. The treatment of NAA 2 mg.l-1 + BAP 1 mg.l-1 gave effect tending to be faster when callus appeared with an average of 75.75 HST, NAA 1 mg.l-1 + BAP 2 mg.l-1 m. and tends to be larger in callus diameter with an average of 0.9033 g and 19.133 mm and NAA 1 mg.l-1 + BAP 1 mg.l-1 gives a higher influence on the percentage of callus growth by an average of 100 %.
Comparación analgésica del bloqueo TAP con bupivacaina y dexmedetomidina, frente al tramadol y metamizol en OVH.
Authors: Ortega Ortega Luis Dirney , Rubio Arias Pablo Giovanny , Castillo Hidalgo Edy Paul 
Published: March 2024

Introducción. Actualmente para el manejo multimodal del dolor se realiza el bloqueo de nerviosos eco guiado, esta es una herramienta de anestesia regional que es de suma importancia dentro de la práctica clínico y quirúrgica. Lo que se busca es desensibilizar determinadas secciones del organismo animal, el uso de anestésicos locales aporta analgesia y reduce la demanda anestésica de los pacientes, Por ende, lo que buscamos es evitar cualquier tipo de sufrimiento a consecuencia del dolor y como profesionales debemos garantizar que nuestros pacientes no experimenten dicha sensación. Objetivo. El objetivo de la presente investigación fue determinar la, analgésica del bloqueo TAP con bupivacaina y dexmedetomidina, frente al tramadol y metamizol en OVH. Metodología. En este trabajo de investigación evaluamos si el bloqueo del trasverso abdominal (TAP) con bupivacaina y dexmedetomidina es más eficiente al momento de controlar el dolor, frente a la administración de metamizol sódico y tramadol intravenoso. Los pacientes fueron seleccionados previamente y se tomó en cuenta que tengan un peso de entre 5-10 kg, los pacientes fueron enlistados en dos grupos y los tratamientos se asignaron al azar. El bloque TAP se realizó bilateralmente empleando el Ultrasonido Chison ECO6 Doppler Color de fabricación China del año 2019, para lo cual se usó un transductor lineal Chison de 10 MHz de fabricación China. Para depositar la bupivacaina (2,5 mg/Kg) combinada con la dexmedetomidina (100 ug/kg) se utilizó agujas ecogénicas Stimuplex® de B-Braun de fabricación alemana. El grupo de individuos que no se les realizo el bloqueo fueron medicados para el dolor posoperatorio por vía intravenosa con tramadol (6mg/kg) más metamizol sódico (40mg/kg). El grado de dolor se evaluó mediante la escala de dolor de Glasgow durante un periodo de 12 horas en pacientes sometidas a ovariohisterectomia. Resultados. La percepción del dolor de los dos grupos experimentales a las 12 horas pos cirugía demostraron que no hubieron diferencias estadísticas significativas (p>0,005), entre la analgesia proporcionada por vía IV, en comparación a la realización del bloqueo nervioso de la rama del TAP. Por lo tanto, la hipótesis planteada es de carácter nulo. Conclusión. Se concluye que no hubo diferencias estadísticas significativas en la percepción del dolor en los dos grupos experimentales, por lo tanto la realización de bloqueos de ramas nerviosas periféricas es segura y factible, ya que ayuda en el manejo multimodal del dolor y de esta manera mejoramos el bienestar de nuestros pacientes, cabe señalar que es una técnica muy utilizada en medicina humana y hoy en día la realización de esta técnica en medicina veterinaria es factible y demuestra grandes resultados. Área de estudio: (Medicina Veterinaria.)
ANTIMICROBIAL ACTIVITY OF NUTMEG (Myristica fragrans Houtt) FRUIT METHANOL EXTRACT AGAINST GROWTH Staphylococus aureus AND Escherichia coli
Authors: Nurhasanah Nurhasanah 
Published: March 2024

Nutmeg (Mirystica fragrans Houtt) were one of famous spices comes from North of Mollucas. Nutmeg have many important part of itself for economic value especially it mace and seed but it fruit mostly unusefull. Chemical compounds whithin nutmeg fruit such atsiri oil have a promising component as an antimicrobial compound. The aim of this research were to determine antimicrobial activity and minimum inhibition concentration of nutmeg fruit againts growth of Staphylococus aureus and Escherichia coli by using metanol as a solvent. The study were conducted at PMIPA FKIP laboratory of Khairun Univercity. This was an experimental research by using completed randomized design which consist of fifteen concentration with three replication. Statistical analizing showed that Fhit Ftab (61,91) for S.aureus whereas 3, 81 for E.coli after Â applyingÂ methanol Â extractÂ of nutmegÂ fruitÂ for those Â bacterial Â growth,Â respectively. Neither for minimum inhibition concetration, S.aureus have a low MIC value (8%) than E.coli (10%), respectively). In conclusion extract of nutmeg fruit by using methanol had ability to inhibit the growth of S.aureus dan E.coli with MIC of S.aureus lower than MIC of E.coli.
JUMLAH DAN DISTRIBUSI STOMATA PADA TANAMAN PENGHIJAUAN DI KOTA TERNATE
Authors: Papuangan Nurmaya , . Nurhasanah , Djurumudi Mudmainah 
Published: March 2024

Plant can absorb Â pollutan. Â Part of Â plant Â which Â absorb Â the Â pollutan Â were leaf. Plant can use as Â pollotan Â indicator Â such Â plant Â which Â growthÂ aside Â street.The Â purpose Â of Â this Â studyÂ were Â to determine the number and distribution of stomatal of plants which grouth aside street at Ternate City. This research was a descriptive study. The data were collecting by couting the number of stomatal and its distribution by using replica and whole mount method. Such plants were used in this study were Ficus benjamina L, Â Callophylum Â inophyllum Â L., Â Acacia crassicarpa, Samanea saman and Arecaceae. The result of this study showed a varies of stomatal number and Â its distribution within five species plants Stomatal number on A. crassicarpa categorited into uncoonting both of Â upper Â adaxial and lower abaxial,Â Â Â F. benjamina L. had Â stomatal number very high at abaxial side than adaxial side, C. inophyllum L. had stomatal number on adaxial side higher than adaxial side in S. Saman, its stomatal number on adaxial side categorized uncoonting whereas adaxial side were low. The stomata number of Arecaceae were categorized moderate on abaxial side where as adaxial side were low. Stomatal distribution on F. benjamina L., C. inophyllum L., A. crassicarpa, S. saman were random wereas Arecaceae were aline.Â
KAJIAN ANTROPOGENIK TERHADAP PEMANFAATAN TERUMBU KARANG DI DESA WOSI, HALMAHERA SELATAN (Suatu kajian kerusakan terumbu karang di daerah pesisir Halmahera)
Authors: Ahmad Zulkifli , Majid Ilham , Jaman Hardi R. 
Published: March 2024

Terumbu karang merupakan salah satu sumber daya alam yang banyak dimanfaatkan oleh masyarakat yang berada di daerah pesisir Halmahera. Salah satu pemanfaatan oleh masyarakat Desa Wosi, Halmahera Selatan adalah sebagian besar dimanfaatkan sebagai bahan bangunan danÂ pondasi rumah. Â Tujuan Â dari Â penelitianÂ iniÂ adalah Â untuk Â mengetahui Â pengetahuan Â dan Â aktivitas Â masyarakat terhadap pemanfaatan terumbu karang di Desa Wosi Kecamatan Gane Timur Kabupaten Halmahera Selatan. Instrumen pengambilan data dilakukan dengan menggunakan angket/kuesioner dan wawancara denganÂ Â sejumlahÂ Â masyarakat.Â Â AnalisisÂ Â angket/kuesionerÂ Â dilakukanÂ Â denganÂ Â menggunakanÂ Â rumus prosentase dan skala Likert. Hasil penelitian menunjukan bahwa; (1) Pengetahuan masyarakat terhadap terumbu karang di Desa Wosi Kecamatan Gane Timur termasuk dalam kriteria cukup Â yaitu dengan nilai rataâ€“rata Â 60%; (2) Â Aktivitas Â masyarakat Â terhadap Â pemanfaatan Â terumbu Â karang Â meliputi ;Â Â Â Indikator pemanfaatan terumbu karang sebagai bahan bangunan dan pondasi rumah termasuk dalam kriteria cukup (nilai rata-rata 63%), indikator jual beli terumbu karang termasuk dalam kriteria rendahÂ (52%), dan budidaya terumbu karang termasuk dalam kriteria sangat rendah (3.23%). Sementara pada kriteria skala Likert Â untukÂ seluruh Â indikator Â pengetahuan Â dan Â aktivitas Â pemanfaatan Â terumbu Â karang Â menunjukan kriteria sangat tinggi (X 4,531 atau 127.242 4.531 dan X 3,5685 atau 97.742 3.5685).Â Kata kunci : Antropogenik, Halmahera Selatan, kerusakan, pemanfaatan, terumbu karang
Genetic identification and expression optimization of a novel protease HapR from Bacillus velezensis
Authors: Han Zhenying , Ye Changwen , Dong Xinyu , Chen Chenchen , Zou Dian , Huang Kuo , Wei Xuetuan 
Published: March 2024

Due to the broad application and substantial market demand for proteases, it was vital to explore the novel and efficient protease resources. The aim of this study was to identify the novel protease for tobacco protein degradation and optimize the expression levels. Firstly, the tobacco protein was used as the sole nitrogen resource for isolation of protease-producing strains, and a strain with high protease production ability was obtained, identified as Bacillus velezensis WH-7. Then, the whole genome sequencing was conducted on the strain B. velezensis WH-7, and 7 proteases genes were mined by gene annotation analysis. By further heterologous expression of the 7 protease genes, the key protease HapR was identified with the highest protease activity (144.19 U/mL). Moreover, the catalysis mechanism of HapR was explained by amino acid sequence analysis. The expression levels of protease HapR were further improved through optimization of promoter, signal peptide and host strain, and the maximum protease activity reaced 384.27 U/mL in WX-02/pHY-P43-SPyfkD-hapR, increased by 167% than that of initial recombinant strain HZ/pHY-P43-SPhapR-hapR. This study identified a novel protease HapR and the expression level was significantly improved, which provided an important enzyme resource for the development of enzyme preparations in tobacco protein degradation.
A novel approach for adsorption of organic dyes from aqueous solutions using a sodium alginate/titanium dioxide nanowire doped with zirconium cryogel beads
Authors: Azeroual Sana , Khatib Khalid , Belfkira Ahmed , Ablouh El-Houssaine , Hanani Zouhair , Taourirte Moha , Jalal Rachid 
Published: March 2024

The presence of organic dyes in wastewater raises significant environmental and human health concerns, owing to their high toxicity. In light of this, a novel adsorbent material with porous cryogel architecture was developed and employed for the effective removal of organic dyes from an aqueous solution. Initially, a titanium dioxide nanowire doped with zirconium HZTO was synthesized by the hydrothermal process. Subsequently, the beads (SA/HZTO) of sodium alginate and HZTO were successfully prepared through a cross-linking process, employing Ca2+ ions as the crosslinking agent. Structural analysis of SA/HZTO beads was performed using FTIR, SEM, and EDX techniques. We systematically examined the impact of different conditions, including the initial dye concentration, pH, contact time, and adsorbent dosage, on the adsorption process. Batch experiments, both in signal and binary systems, were conducted to rigorously assess the dye adsorption capabilities. Kinetic modeling revealed that the adsorption process adhered to the pseudo-second-order kinetic model. Remarkably, the prepared beads exhibited impressive adsorption capacities of 26 and 29 mg/g toward methylene blue (MB) and safranin (SF), respectively. SA/HZTO beads have demonstrated excellent adsorption properties, offering a promising avenue for the development of low-cost, efficient, and reusable adsorbent to remove dyes from wastewater.
Age differences in the association of body mass index-defined obesity with abdominal aortic calcification
Authors: Guo Tangmeng , Huang Lili , Luo Zhijian , Zheng Huabo , Shan Shengshuai , Cheng Bei 
Published: March 2024

ObjectivesIn cardiovascular disease, previous studies have suggested young age as one of the reasons to explain the obesity paradox. This study attempts to provide a different opinion on this claim through unexpected findings.MethodsWe used a cross-sectional analysis of the US nationally representative data, total of 10,175 participants were recruited in 2013-2014 from NHANES. A total of 947 participants were selected to be included in this study through inclusion criteria and exclusion criteria for statistical analysis of the relationship between obesity and abdominal aortic calcification(AAC). Smooth curve fitting and multivariate regression analyses were conducted to examine the associations of obesity with AAC after adjusting for age, gender and associated variates.ResultsDepending on the age of the population, the relationship between obesity and AAC showed the different outcome. Obesity was associated with the lower risk of AAC among individuals older than 52 years of age. According to the difference of adjusted covariates, the AAC scores in the obesity group decreased by 0.92, 0.87, and 1.11 for 52 years old or older individuals. In particular, the risk of AAC was lower for patients with obesity with the following characteristics: male, low LDL, low triglyceride, DM, non-cancer patient, smoking, drinking, vigorous work activity, low annual household income, education of 9 – 11th grades and non-Hispanic white.ConclusionsIn US, adults aged 52 years or older, obesity was associated with decreased AAC risk. Older age may be one potential reason for the obesity paradox.
Comparative study between poorly differentiated thyroid cancer and anaplastic thyroid cancer: real-world pathological distribution, death attribution, and prognostic factor estimation
Authors: Zhang Kun , Wang Xinyi , Wei Tao , Li Zhihui , Zhu Jingqiang , Chen Ya-Wen 
Published: March 2024

BackgroundThe clinic-pathological boundary between poorly differentiated thyroid cancer (PDTC) and anaplastic thyroid cancer (ATC) is unclear due to a wide spectrum of histopathological features and the rarity of the disease. In addition to that, with the highest mortality rate and non-standard treatment modality, the PDTC/ATC population has not been subjected to comprehensive description and comparison with the extent of histological characteristics, therapeutic response, prognostic factors, and death attribution analysis.MethodA total of 4,947 PDTC/ATC patients from 2000 to 2018 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. Kaplan–Meier survival curve estimation and Cox proportional hazard regression were applied.ResultsOverall, the 5- and 10-year DSS for PDTC were 71.9% and 68.0%, respectively, whereas the 5- and 10-year OS are 59.3% and 51.2%, respectively. The median survival time for ATC patients was 3 months with 1-year OS being 26.9% and 1-year DSS being 31.2%. During the follow-up period, 68.1% of the PDTC/ATC cohort were dead, 51.6% of which were attributed to thyroid malignancies and 16.5% to non-thyroid causes. The top three common non-thyroid causes of death were miscellaneous cancers, lower respiratory system disease, and heart disease. The histological feature of papillary thyroid cancer (PTC) was the leading pathological category for PDTC patients (51.7%), whereas 76.7% of ATC patients’ pathological feature was characterized as unidentifiable. Sarcoma histological characteristics found in ATC cases suffer the highest overall mortality (vs. PTC, HR = 2.61, 95% CI 1.68–4.06, P &lt; 0.001). Older age unidentifiable histology feature, more advanced AJCC N1b, AJCC M1, and SEER stage, tumor size larger than 5 cm, and more invasive tumor extension were independent bad outcome predictors.ConclusionThe populational analysis of the PDTC/ATC cohort has provided reliable support for better understanding of the difference between PDTC and ATC cases and the guidance of clinical practice and further studies.
A two-phase, multi-component model for efficient CO2 storage and enhanced gas recovery in low permeability reservoirs
Authors: Wang Xiangzeng , Zhang Quan , Wan Yongping 
Published: March 2024

Introduction: Carbon dioxide (CO2) enhanced gas recovery represents a viable strategy for sequestering CO2 while concurrently augmenting gas production from subsurface reservoirs. Gas reservoirs, as inherent geological formations, are optimal repositories for gaseous compounds, rendering them suitable for CO2 storage. Nevertheless, the economic viability of pure CO2 storage necessitates integration with oil and gas recovery mechanisms to facilitate widespread CO2 utilization.Method: This study addresses the complexities of CO2 enhanced gas recovery through a comprehensive approach that combines theoretical model and numerical simulations. A numerical model is developed to simulate three-component diffusion involving CO2, and methane (CH4) in a two-phase system comprising gas and water.Results: The investigation systematically explores the process of enhanced CH4 extraction and CO2 injection into the reservoir and examines the influencing factors on extraction. Simulation results reveal a power-law decrease in CH4 production rate, stabilizing at a constant extraction rate. Enhanced CH4 extraction benefits from increased porosity, with higher porosity levels leading to greater CH4 extraction. Permeability augmentation positively influences CH4 production, although with diminishing returns beyond a certain threshold. The CO2 injection rate shows a direct proportionality to CH4 production. However, elevated CO2 injection rates may increase reservoir pressure, potentially causing cap rock damage and CO2 gas flushing.Discussion: This study contributes valuable theoretical insights to the field of CO2 enhanced gas recovery engineering, shedding light on the intricate dynamics of multi-component fluid transport processes and their implications for sustainable CO2 utilization.
Association of glutamate receptor gene polymorphisms with attention-deficit hyperactivity disorder susceptibility: a systematic review and meta-analysis
Authors: Zou Dehua , Zeng Qiaoli , Liu Pei , Wei Yue , Guo Runmin , Zhu Yizhun , He Rong-Rong 
Published: March 2024

Background: There is a growing body of evidence indicating a possible association between genetic variations and attention-deficit hyperactivity disorder (ADHD), although the results have been inconsistent. The objective of this study was to evaluate the correlation between the GRIN2A, GRIN2B and GRM7 gene polymorphisms and ADHD.Methods: A comprehensive meta-analysis and subgroup evaluation was conducted using a fixed-effects model to analyze the association between ADHD and GRIN2B (rs2284411), GRIN2A (rs2229193), and GRM7 (rs3792452) in six genetic models (dominant, recessive, overdominant, homozygous, heterozygous, and allele models).Results: The meta-analysis comprised 8 studies. The overall analysis showed that the GRIN2B rs2284411 T allele and T carries were significantly associated with a decreased risk of ADHD (dominant model:TT + CT vs. CC: OR = 0.783; 95% CI: 0.627–0.980; p = 0.032, allele model:T vs. C: OR = 0.795; 95% CI: 0.656–0.964; p = 0.019), especially in the Korean subgroup (dominant model:TT + CT vs. CC: OR = 0.640; 95% CI: 0.442–0.928; p = 0.019, overdominant model: CT vs. TT + CC: OR = 0.641; 95% CI: 0.438–0.938; p = 0.022, allele model:T vs. C: OR = 0.712; 95% CI: 0.521–0.974; p = 0.034 and heterozygous model: CT vs. CC: OR = 0.630; 95% CI: 0.429–0.925; p = 0.018). However, no meaningful associations were found for rs2229193 and rs3792452.Conclusion: The results of the meta-analysis provide strong evidence that the rs2284411 T allele is significantly associated with reduced susceptibility to ADHD, particularly in the Korean population.