bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-omics analysis unravels the underlying mechanisms of the escape response of the common strain black carp
Authors: Xu Yuanfeng , Dong Ping , Sewo Daniel Yohannes , Su Shengyan , Li Jianlin , Feng Wenrong , Liao Yu , Tang Yongkai 
Published: February 2024

Abstract
Reducing the escape response of fish during the aquaculture has important economic value and ecological safety significance. This study takes the common strain black carp (Cyprinus carpio var. baisenensis), which is known for its low-escape response, as the main research object. Through a simulated flood experiment, the common strain black carps were divided into the low-escape (BL) group and the high-escape (BH) group. Multi-omics techniques:transcriptome sequencing, LS-MS/MS detection, and 16s sequencing were used to analyze the differences in brain gene transcription levels, liver metabolites, and intestinal microbiota composition between the two groups. In addition, in order to reduce false positives generated by transcriptome experiments, Jian carps (Cyprinus carpio var. Jian) were also grouped into the low-escape (JL) group and the high-escape (JH) group and subjected to transcriptome analysis. According to the differentially expressed genes (DEGs) analysis, 18 DEGs were obtained from both the common strain black carp and Jian carp. Except for the MAP6 gene, which was significantly highly expressed, the remaining 17 genes showed significantly low expression in the BL group, which were almost related to signal transduction, and brain tissue and neuronal development. The results of KEGG signaling pathway annotation, KOG functional annotation, and topGO enrichment analysis showed that there were significant differences in signal transduction between the BL and BH groups, especially in the gama aminobutyric acid (GABA) signaling pathway. The detection of liver metabolites showed that there were differences in tryptophan metabolism between the BL and BH groups of the common strain black carp, with higher tryptophan content in the liver of the BL group fish. This study suggests that the brain activity and development of low-escape fish may be lower than that of high-escape fish. Meanwhile, differences in the brain's GABA signaling pathway and the liver's tryptophan metabolismmay also affect fish's escape response. This study accumulates experimental foundation of fish escape response, and provides a new insight into breeding low-escape fish and developing novel strategies to reduce escape responses during aquaculture.
Greedy Recursive Spectral Bisection for Modularity-bound Hierarchical Divisive Community Detection
Authors: Cardoso Douglas O. , Junior João Domingos Gomes da Silva , Oliveira Carla Silva , Marques Celso , de Assis Laura Silva 
Published: February 2024

Abstract

Spectral clustering refers to a class of techniques that depend on the eigenstructure of a similarity matrix for the purpose of dividing data points into disjoint clusters, where points within the same cluster exhibit high similarity and those in different clusters have a lower similarity. The objective of this work was to develop a spectral method that could be compared to clustering algorithms which represent the current state of art. This investigation conceived a novel spectral clustering method, as well as 5 policies which guide its execution, based on spectral graph theory and embodying hierarchical clustering principles. Computational experiments were undertaken in this study to evaluate the clustering methods under scrutiny. The assessment was performed using two evaluation metrics, specifically the adjusted Rand index, and modularity. The obtained results furnish compelling evidence, indicating that the proposed method is competitive and possesses distinctive properties compared to those elucidated in the existing literature. This suggests that our approach stands as a viable alternative, offering a robust choice within the spectrum of available same-purpose tools.
Co-culture with bronchial epithelial cells and hypoxia promote ADAM33 expression to regulate activation, ferroptosis, proliferation, migration, and apoptosis of embryonic lung fibroblasts
Authors: aierken haidiya , Wang Jing , Wufuer Dilinuer 
Published: February 2024

Abstract

Asthma is a common chronic respiratory system disease, and thoroughly revealing its pathological mechanism is still a major challenge in the current biomedical field. Studies on embryonic lung fibroblasts have found that hypoxia promoted the expression and secretion of ADAM33 in a time-dependent manner. Co-culture with bronchial epithelial cells (co-BECs) also induced the expression of ADAM33, and hypoxia further enhanced the effect of co-culture on promoting ADAM33 expression. Western blot and/or immunofluorescence detection showed that co-BECs significantly enhanced the expression of α-SMA, vimentin, calponin, collagen I and vinculin, but the effect on promoting desmin expression was not significant; ADAM33 overexpression or hypoxia further enhanced these gene expression, and ADAM33 overexpression plus hypoxia had the most significant expression-promoting effect; however, ADAM33 knockout blocked the promotion effect of co-culture on these gene expression, indicating that co-culture promotes fibroblast activation through ADAM33. Further detection of ferroptosis showed that co-BECs promoted the expression of GPX4 and FPN, and inhibited the expression of ACSL4; ADAM33 knockout down-regulated the expression of GPX4, FPN, FTH1 and FTL, while ADAM33 overexpression or hypoxia enhanced the regulatory effect of co-culture on these gene expression, and the synergistic effect of ADAM33 overexpression and hypoxia on co-culture was more significant. In addition, co-BECs promoted fibroblast proliferation and migration, increased GSH content, and reduced MDA production and cell apoptosis; ADAM33 knockout significantly increased the accumulation of Fe2+, ROS and MDA, as well as cell apoptosis, while reducing GSH content, cell proliferation and migration; ADAM33 overexpression or hypoxia had opposite effects, and ADAM33 overexpression plus hypoxia had a more significant regulatory effect on these biological traits of fibroblasts. Therefore, epithelial cells can regulate the activation, ferroptosis, proliferation, migration and apoptosis of fibroblasts by enhancing the expression of ADAM33, indicating that ADAM33 plays an important role in fibroblasts.
Emergency Surgery Score (ESS) In Predicting Post- Operative Course In Patients Undergoing Emergency Laparotomy – An Indian Tertiary center experience
Authors: Bhowmik Srishti , Singh Chandra Bhushan , Neogi Sushanto , Ghuliani Deepak , Roy Sarmista 
Published: February 2024

Abstract
Aims And Objectives - To determine the predictive value of Emergency Surgery Score (ESS) with regards to mortality and morbidity rates of patients undergoing emergency laparotomy. Method - The ESS, ranging from 0 to 29 is an extensive risk calculator based on 22 variables including important parameters like demographics, pre-operative treatment, comorbidities, and laboratory values. Twenty patients who underwent emergency laparotomy were preoperatively assessed and ESS was calculated for each. After establishment of diagnosis and resuscitation, the patient was taken up for emergency laparotomy. Postoperatively, patients were monitored clinically as well as with laboratory and radiological investigations as per case needed till discharge and further followed up physically in OPD/ ward or interviewed telephonically for 30 days on a weekly basis. Incidence of mortality and morbidity in terms of postoperative complications, ICU admission, reoperation and readmission among the cases occurring within 30 days of procedure were recorded. Results - ESS correlated well with the outcome in the current study, 10 out of 14 patients with score less than 8 were discharged without any complications. Mean ESS was higher among non survivors. Ability of ESS to predict postoperative mortality, morbidity and ICU stay was proven statistically with c-statistics of 0.853, 0.84, 0.879 respectively. ESS was found to be good predictor for development of postoperative LRTI (c-statistic = 0.828), sepsis (c-statistic = 0.867), DIC (c-statistic = 0.805), AKI (c-statistic = 0.804). ESS showed poor correlation with reoperation and readmission rates. Conclusion - It can be concluded from the study that risk stratification should be done for patients undergoing emergency laparotomy by using ESS. Thereby it can predict necessary needs for resuscitation and patient stabilization before surgery. Using ESS, patients unsuitable for operation can be identified and optimized beforehand; well informed decisions can be taken regarding targeted treatment, operation, and post-operative care. This study is a registered study under Clinical Trials Registry - India (CTRI) with registration number CTRI/2021/07/034504 and was registered on 01/07/2021.
Anti-sarcopenic effect of leucine-enriched branched-chain amino acid supplementation among elderly chronic kidney disease patients: a double-blinded randomized controlled trial
Authors: Sunsandee Neti , Thimachai Paramat , Satirapoj Bancha , Supasyndh Ouppatham 
Published: February 2024

Abstract
Background
Leucine, branched-chain amino acids (BCAA), represents an effective nutritional strategy to augment skeletal muscle mass in aging population. The study aimed to determine the effect of oral leucine-enriched BCAA supplementation on muscle mass, biomarkers of muscle synthesis and physical performance among elderly patients with chronic kidney disease (CKD).
Methods
A randomized controlled trial study was performed among patients with CKD stage III and IV aged over 65 year. Eligible participants were randomly assigned to either oral 4.5 g/day of leucine in mixed BCAA supplement (N = 29) or placebo (N = 26) in the same manner for 12 weeks. Blood chemistry, serum insulin growth factor-1 (IGF-1), serum myostatin, muscle mass using dual energy X-ray absorptiometry were measured at start and end of the intervention. Three-day food record was documented and reviewed by a dietitian. All participants were monitored for functional capacity using the handgrip and 6-minute walk tests during the study.
Results
Fifty-five patients (33 males) with mean age of 75.4 ± 5.2 years were enrolled. Daily protein and calorie intake during the study were similar in both groups. After the end of the study, lean muscle mass was significantly increased in the leucine group compared with that of the placebo group (0.4 kg (95% CI 0.1 to 0.7) vs. -0.2 kg; 95% CI -0.6 to 0.2) kg, P = 0.010, respectively). Also, statistically significant differences were noted in percentage of changed in muscle mass at 12 weeks between the leucine and placebo groups (1.0 ± 1.8 vs. -0.5 ± 2.6%, P = 0.014). However, muscle strength determined by handgrip and 6-minute walk tests, serum myostatin and IGF-1 did not significantly differ between the two groups. No serious adverse event was observed during the study.
Conclusions
In our study, 12 weeks of oral supplement with leucine-enriched BCAA resulted in increasing muscle mass, However, the treatment dose showed no benefit on biomarkers of muscle activity, functional capacity and adverse reaction among elderly patients with CKD.
EDIL3 alleviates Mannan-induced psoriatic arthritis by slowing the intracellular glycolysis process in mononuclear-derived dendritic cells
Authors: Yu Jiadong , Wang Xiaoyan , Zhou Yifan , Hu Jing , Gu Linna , Zhou Hong , Yue Chengcheng , Zhou Pei , Li Ya , Zhao Qixiang , Zhang Chen , Hu Yawen , Zeng Fanlian , Zhao Fulei , Li Guolin , Feng Yuting , Wu Wenling , Huang Nongyu , Cui Kaijun , Li Jiong 
Published: February 2024

Abstract

Psoriatic arthritis (PsA) is an immune-mediated, chronic inflammatory joint disease that commonly occurs as a complication of psoriasis. EGF-like repeats and discoidal I-like domain 3 (EDIL3) is a secreted protein with multiple structural domains and associated with various physiological functions. Mannan-induced psoriasis arthritis-like disease relies on the differentiation of MoDCs and Th17 cells. Reduced expression of EDIL3 was correlated with more severe symptoms in mannan-induced PsA. Additionally, EDIL3 knockout mice also exhibited more severe PsA disease, which could be reversed by reinfusion of recombinant EDIL3 protein. The alleviating effect of EDIL3 on PsA depends on the activation of MoDCs and Th17 cells. After neutralizing antibodies against MoDCs and Th17 cells were used to inhibit their function, the beneficial effect of EDIL3 on PsA was lost. Furthermore, EDIL3 can activate AMPK phosphorylation while reducing AKT phosphorylation, thereby slowing down mannose-induced intracellular glycolysis process in MoDCs and inhibiting their maturation and differentiation. Consequently, these compounds reduce the differentiation of Th17 cells and slow down the progression of PsA. Our results elucidate the role and mechanism through which EDIL3 contributes to the development of PsA, providing new targets for clinical diagnosis and treatment.
Development and evaluation of a deep learning framework for detection and diagnosis of peri-ampullary tumor in MRI images
Authors: Tang Yong , Zheng Yingjun , Luo De , Zhou Linjing , Wang Weijia , Wang Xuewen , Chen Jing , Li Bo , Shu Jian , Lv Muhan , Wu Jiali , Su Song 
Published: February 2024

Abstract
Purpose: We were development and evaluation of one deep learning (DL) framework for identification of Peri-ampullary (PA) regions and diagnosis of peri-ampullary tumor (PAT) conditions in MRI images.
Experimental Design: We retrospectively analyzed 1,038 patients. We found that DL algorithm for identification of PA regions and diagnosis of PAT conditions in MRI images.
Results: The DL algorithm successfully identified and segmented the PA regions in both T1WI (IOU = 0·62) and T2WI images (IOU = 0·55). Based on the segmentations of PA regions in images, the classification DL algorithm achieved optimal accuracies in classifications of NPA and PSOL with AUC of 0·71 (95% CI 0·68 to 0·74) (T1WI) and 0·72 (95% CI 0·68 to 0·75) (T2WI). For PSOL cases, another classification DL algorithm achieved encouraging accuracies to further classify PAT and Non-PATL with AUC of 0·81 (95% CI 0·77 to 0·85) (T1WI) and 0·78 (95% CI 0·73 to 0·83) (T2WI). Furthermore, in the patient-based approach, the classification DL algorithm achieved optimal accuracies in classifications of NPA and PSOL with ACC of 0·75 (95% CI 0·65 to 0·85) (T1WI) and 0·88 (95% CI 0·81 to 0·94) (T2WI). For PSOL cases, another classification DL algorithm achieved encouraging accuracies to further classify PAT and Non-PATL with ACC of 0·83 (95% CI 0·71 to 0·96) (T1WI) and 0·82 (95% CI 0·70 to 0·93) (T2WI).
Conclusions: Our study suggests that DL could accurately identify and segment PA regions in MRI images and further classify PAT conditions with promising accuracies. DL could assist clinicians in MRI interpretation for PAT diagnosis.
Adaptive Adversarial Augmentation for Molecular Property Prediction
Authors: Cho Soyoung , Hong Sungchul , Jeon Jong-June 
Published: February 2024

Abstract
Graph Neural Networks (GNNs) exhibit potential in predicting the properties of molecules, but computational analyses with the GNNs often encounter the problem of data imbalance or overfitting. Augmentation techniques have emerged as a popular solution, and adversarial perturbation to node features achieves a significant improvement in enhancing the model's generalization capacity. Despite remarkable advancement, there is scarce research about systematically tuning the adversarial augmentation. We propose a new framework for an adversarial perturbation with influential graph features. Our method selects the data to apply adversarial augmentation based on the one-step influence function that measures the influence of each training sample on prediction in each iteration. In particular, the approximation of the one-step influence function has wide applicability to evaluate a model's validity on the observation level for a large-scale neural network. Selected data using the one-step influence function are likely to be located near the decision boundary, and experimental results demonstrated that augmentation of such data has improved the model's performance.
An unexpected case of tetanus in a fully immunized 20-year-old female: A case report
Authors: Okuda Mitsutoshi , Morizane Atsushi , Asaba Sunao , Tsurui Saika , Tsuno Ryutaro , Hatakenaka Mariko , Sugimura Tomoko , Saisaka Yuichi 
Published: February 2024

Abstract

Background:
Widespread vaccinations have significantly decreased the number of tetanus cases in developed countries. Today, most cases of tetanus affect the elderly and those with inadequate immunization in developed countries such as Japan. As vaccinations were believed to be nearly 100% effective in preventing tetanus, tetanus in young, immunized individuals were considered unlikely. However, unexpected tetanus infection has been reported in young adequately immunized individuals.
Case:
We herein describe a 20-year-old immunized female who visited our emergency department with trismus and painful muscle spasms that started after receiving a puncture wound to her right foot. A physical examination revealed an elevated body temperature (38°C), trismus, muscle spasms in her right leg and neck, and a puncture wound at the sole of her right foot. Following the development of dyspnea after admission to the intensive care unit, the patient was intubated and mechanically ventilated. She fully recovered after six days in intensive care.
Conclusion:
The present case serves as a stark reminder that tetanus may still occur in young, immunized individuals. Patients with a history of immunization may have a better prognosis than those with no immunizations.
SIDM-WIR: A Sampling Improved DiffusionModel for Worksite Image Restoration
Authors: Wen JiaYan , Zhuang Yuansheng , Deng JunYi 
Published: February 2024

Abstract
At presently, Diffusion Model (DM) has achieved state-of-the-art performanceby modeling the image synthesis process through a series of denoising networkapplications. Image Restoration (IR) is to improve the subjective image qual-ity corrupted by various kinds of degradation unlike image synthesis. However,IR for worksite images is greatly challenging in the low-level vision field due tocomplicated environmental factors. To solve this problem, we propose a Sam-pling Improved Diffusion Model for Worksite Image Restoration (SIDM-WIR).It not only improves the authenticity and representation ability for the gener-ation process, but also handles complex backgrounds and diverse object typeseffectively. SIDM-WIR has three main contributions: 1) Its framework adopts aMish-based residual module, which enhances the ability to learn complex patternsof images, and allows for the presence of negative gradients to reduce overfittingrisks during model training. 2) It employs a Mixed-head Self-Attention (MSA)mechanism, which augments the correlation among input elements at each timestep, and maintains a better balance between capturing the global structuralinformation and local detailed textures of the image. 3) It ran in a new customizeddataset named Workplace for WIR specifically. Furthermore, experiments on bothtwo datasets demonstrate that SIDM-WIR achieves better results to previousmethods both quantitatively and qualitatively.
Results of biosimilar assessing of insulin aspart preparations of Russian and foreign production using the hyperinsulinemic euglycemic clamp method and studying the use of a new ultra-fast-acting Russian biosimilar in pumps
Authors: Saverskaya E. N. , Koksharova E. O. , Noskov S. M. , Zaikin P. G. , Banko V. V. , Arefeva A.  N. 
Published: February 2024

Introduction. Ultra-fast-acting insulin aspart has great potential for improving postprandial glycemia in patients with type 1 and type 2 diabetes mellitus due to its pharmacological characteristics. The development and production of biosimilars are increasing the availability of modern insulins for patients.Aim. To evaluate the comparability of the pharmacokinetics and pharmacodynamics profiles of insulin aspart GP40311 (tested biosimilar of domestic production) and the reference drug (produced in Denmark) under conditions of a hyperinsulinemic euglycemic clamp in healthy volunteers. To evaluate the stability of a new ultrafast-acting biosimilar when used for continuous subcutaneous infusion in insulin pumps.Materials and methods. Double-blind, randomized, crossover study assessing the pharmacokinetics, pharmacodynamics and safety of the tested biosimilar GP40311 of domestic production and the reference drug produced in Denmark, in the form of a solution for intravenous and subcutaneous administration of 100 IU/ml, the study was conducted under conditions of a hyperinsulinemic euglycemic clamp with the participation of 36 healthy volunteers. A study of the stability, dosing accuracy and tendency to catheter occlusion of a domestic drug for continuous subcutaneous infusion was carried out using several types of insulin pumps using the gravimetric method for 72 hours. Dosing accuracy was determined at the minimum and maximum bolus dose, stability was assessed by pH and quantitative insulin content aspart. The quantitative content of insulin and impurities was assessed by high-performance liquid chromatography.Results and discission. The 90% confidence interval for the ratio of geometric mean values of the main parameters of pharmacokinetics (AUCins.0-t and Cins.max) of insulin aspart test and reference drugs corresponded to the acceptable values of 80.00– 125.00%, which indicated their biosimilarity. When assessing PD, the comparability of action parameters is shown. The safety of the study drugs is comparable. Domestic insulin aspart met the specification standards when used for continuous subcutaneous infusion according to physicochemical parameters: pH, quantitative determination of insulin aspart, impurity content. The accuracy of dosing and the absence of occlusions in systems for 72 hours when using the drug in pumps have been established.Conclusion. The study drugs were found to be biosimilar and equally safe. Domestic insulin aspart meets specification standards and can be used in various types of pumps.