bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tocopherol stability in bell pepper microsomal membranes during short‐term storage
Authors: Erickson M. C. , Wang G. D. , Cowart D. M. , Shewfelt R. L. 
Published: March 2007

AbstractSaponification and detergent (sodium dodecyl sulphate) solubilization both assisted in the efficient extraction of α‐tocopherol from bell pepper microsomal membranes by solvent. In contrast, solvent extraction by itself exhibited poor extraction capability. In the presence of dithiothreitol, no losses of α‐tocopherol from microsomal membranes were found within 6 h after suspension of membranes; however, in the absence of dithiothreitol, 82% of the initial α‐tocopherol could not be detected within this same time period. α‐Tocopherol losses were not alleviated by nitrogen flushing of the atmosphere of the membrane, although, lower rates of degradation were observed when suspension of microsomal membrane pellets was delayed. Inclusion of water‐soluble antioxidant during short‐term storage of bell pepper microsomal membranes is recommended and similar precautions may be warranted for membranes from other tissues.
Simultaneous analysis of cytokinins, auxins and abscisic acid by combined immunoaffinity chromatography, high performance liquid chromatography and immunoassay
Authors: Fernández B. , Centeno M. L. , Feito I. , Sánchez‐Tamés R. , Rodríguez A. 
Published: March 2007

AbstractA method has been developed for the rapid and simultaneous extraction and analysis from plant material of 3‐indolylacetic acid (IAA), naphthalene acetic acid (NAA), abscisic acid (ABA) and the cytokinins benzyladenine (BA), zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, isopentenyl adenine and isopentenyl adenosine. The method involves extraction with 80% (v/v) methanol, pre‐purification of the extracts through reversed phase C18 Sep‐Pak cartridges and immunopurification. The separation of the different compounds was accomplished by reverse‐phase high performance liquid chromatography for cytokinins, and by partition with diethyl ether for IAA, NAA and ABA. After methylation of the IAA fraction with diazomethane, quantification of all plant growth regulators (PGRs) was made by immunoassay. The percentage recovery at each step was monitored following the addition of radioactive compounds at the beginning of the process. The final recovery was 68% for IAA, 92% for NAA, 76% for ABA and 75% for BA. The method was applied to the analysis of PGRs in tissues and callus of kiwifruit (Actinidia deliciosa Liang and Ferguson).
Prenatal diagnosis in monozygotic twins with Down syndrome who had different phenotypes
Authors: Grynberg M. , Graesslin O. , Teyssedre J. , Quereux C. , Gaillard D. , Carré‐Pigeon F. 
Published: March 2007

AbstractWe report the case of monozygotic (MZ) male twin fetuses with different Down syndrome (DS) phenotypes. Prenatal fetal sonography showed a bichorial biamniotic pregnancy with increased nuchal translucency in twin A and a cervical cystic hygroma and heart defect in twin B. Cytogenetic analysis performed after double amniocentesis showed free and homogeneous trisomy 21 in both twins. Monozygosity was confirmed by molecular analysis. The pregnancy was terminated at 17 weeks of gestation (WG). Postmortem analysis confirmed the phenotypic discordance. To our knowledge, this is the first reported prenatal diagnosis of MZ male twins with different Down syndrome phenotypes but identical karyotypes. We discuss the mechanisms involved in phenotypic discordance of monozygotic twins and particularly the role of environmental factors. Copyright © 2007 John Wiley & Sons, Ltd.
Prenatal sonographic findings and hematological abnormalities in fetuses with transient abnormal myelopoiesis with Down syndrome
Authors: Hojo Satoshi , Tsukimori Kiyomi , Kitade Shoko , Nakanami Naoyuki , Hikino Shunji , Hara Toshiro , Wake Norio 
Published: March 2007

AbstractObjectivesTo determine relevant prenatal findings of transient abnormal myelopoiesis (TAM) that have important prognostic implications.MethodsThe prenatal and postnatal medical records of all cases with confirmed TAM associated with Down syndrome were reviewed retrospectively, with emphasis on prenatal sonographic findings, fetal blood analysis, neonatal outcomes, and causes of death.ResultsFrom January 1992 to December 2005, seven cases were confirmed postnatally as having TAM associated with Down syndrome. Sonography demonstrated hydrops with hepatomegaly in four, and isolated hepatomegaly in two of these seven cases. There were no findings suggestive of cardiac failure in cases of hydrops. Fetal blood analysis revealed elevated liver enzyme levels in six cases and hypoalbuminemia in four cases. Comparison of sonographic findings with fetal blood findings demonstrated an association between hydrops and hypoalbuminemia. Four of the seven cases were fatal. All fatal cases were associated with hydrops and the main cause of death was coagulopathy due to liver failure, which may have resulted from infiltration of the liver by blast cells.ConclusionsFetal TAM is associated with hepatomegaly and elevated liver enzyme levels. The prenatal finding with prognostic implications is hydrops, which may result from hypoalbuminemia due to liver failure. Copyright © 2007 John Wiley & Sons, Ltd.
Synthesis and helicity of optically active poly(N‐propargylphosphonamidates) having chiral phosphorus center
Authors: Yue Dongmei , Fujii Toru , Terada Kayo , Tabei Junichi , Shiotsuki Masashi , Sanda Fumio , Masuda Toshio 
Published: March 2007

AbstractNovel chiral N‐propargylphosphonamidate monomers (HCCCH2NHP(O)ROmenthyl, 1: R = CH3, 2: R = C2H5, 3: R = n‐C3H7, 4: R = Ph) were synthesized by the reaction of the corresponding phosphonic dichlorides with menthol and propargylamine. Pairs of diastereomeric monomers 1–4 with different ratios were obtained due to the chiral P‐center and menthyl group. One diastereomer could be separated from another one in the cases of monomers 1 and 2. Polymerization of 1–4 with (nbd)Rh+[η6‐C6H5B−(C6H5)3] as a catalyst in CHCl3 gave the polymers with number‐average molecular weights ranging from 5000 to 12,000 in 65–85%. Poly(1)–poly(4) exhibited quantitative cis contents, and much larger specific rotations than 1–4 did in CHCl3. The polymers showed an intense Cotton effect around 325 nm based on the conjugated polyacetylene backbone. It was indicated that the polymers took a helical structure with predominantly one‐handed screw sense, and intramolecular hydrogen bonding between PO and NH of the polymers contributed to the stability of the helical structure. Poly(1a) and poly(2a) decreased the CD intensity upon raising CH3OH content in CHCl3/CH3OH. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 1515–1524, 2007
Monitoring and blunting in palliative and curative radiotherapy consultations
Authors: Timmermans Liesbeth M. , van Zuuren Florence J. , van der Maazen Richard W. M. , Leer Jan Willem H. , Kraaimaat Floris W. 
Published: March 2007

AbstractObjective: The present research paper investigates how cancer patients' monitoring and blunting coping styles are reflected in their communications during their initial radiotherapy consultations and in their evaluations of the consultation. Additionally, it is explored how a patient's disease status (curative versus palliative) influences the effects of his or her cognitive styles.Methods: The study included 116 oncology patients receiving treatment from eight radiation oncologists. For 56 patients treatment intent was palliative and for the remaining 60 curative. The patients' communicative behaviors were assessed using the Roter Interaction Analysis System (RIAS). Within three days the patients completed a monitoring and blunting inventory and after another six weeks they evaluated the treatment decision and treatment information by postal questionnaire.Results: Monitoring was positively and blunting negatively related to the patient's expression of questions, emotions and decision‐making issues. After six weeks ‘high monitors’ as opposed to ‘low monitors’ reported having more doubts about the treatment decision and being less satisfied with the information received while ‘high blunters’ expressed fewer doubts and more satisfaction than ‘low blunters’ did. Significant associations were all attributable to the palliative treatment group.Conclusion: Cancer patients' communicative behaviors vis‐à‐vis their oncologist hinge on their cognitive styles and an unfavorable disease status enhances the effects. Copyright © 2007 John Wiley & Sons, Ltd.
Proteomic expression profiling of thyroid neoplasms
Authors: Braunschweig Till , Kaserer Klaus , Chung Joon‐Yong , Bilke Sven , Krizman David , Knezevic Vladimir , Hewitt Stephen M. 
Published: March 2007

AbstractThyroid cancer is the most common endocrine neoplasm with multiple histologic subtypes, each associated with different treatments and outcomes. Differentiating benign neoplasms such as follicular adenomas from malignant entities such as follicular carcinomas and papillary carcinoma can be challenging. To define the proteomic profile of different thyroid tumors, we screened an antibody array of 330 features against five thyroid neoplasms: follicular adenoma, follicular carcinoma, papillary carcinoma, anaplastic carcinoma, and medullary carcinoma as well as normal thyroid epithelium. Eight candidate biomarkers; c‐erbB‐2, Stat5a, Annexin IV, IL‐11, RARα, FGF7, Caspase 9, and phospho‐c‐myc were identified as differentially expressed on the antibody array, and validated with immunohistochemistry on tissue microarrays, with a total of 144 samples of the same variety of thyroid neoplasms. Analysis revealed c‐erbB‐2, Annexin IV, and Stat5a have potential clinical utility to differentiate follicular adenoma, follicular carcinoma, and papillary carcinoma from each other. By using an antibody array as a discovery platform and a tissue microarray as a first step in validation on a large number of specimens, we have identified new markers that have potential utility in the diagnosis of thyroid neoplasms.
Two‐dimensional gel electrophoresis of apolipoprotein C‐III and other serum glycoproteins for the combined screening of human congenital disorders of O‐ and N‐glycosylation
Authors: Bruneel Arnaud , Robert Tiphaine , Lefeber Dirk J. , Benard Guillaume , Loncle Emilie , Djedour Amel , Durand Geneviève , Seta Nathalie 
Published: March 2007

AbstractCongenital disorders of glycosylation (CDG) are inherited diseases that can affect not only the N‐glycan (e.g. CDG type I and II) but also the O‐glycan biosynthesis pathway. In the absence of specific clinical symptoms, there is a need for a reliable biological screening of these two groups of CDG. Using a few microlitres of human serum, 2‐DE and immunoblotting were applied to the separation and simultaneous detection of the isoforms of the O‐glycosylated protein apolipoprotein C‐III (apoC‐III) and of four N‐glycosylated proteins, namely alpha‐antitrypsin, alpha‐1 acid glycoprotein, haptoglobin and transferrin. For the study of O‐glycosylation, this technique allowed the reliable separation of the three fractions of apoC‐III and the determination of normal percentage values in an adult population. Concerning N‐glycosylation, the study of serum samples from patients with CDG type Ia revealed marked abnormalities systematically affecting the four 2‐DE separated N‐linked glycoproteins. 2‐DE coupled to immunoblotting using a mixture of specific antibodies could be easily and reliably employed for the combined screening of both N‐ and O‐glycosylation disorders in humans.
Discovery of stage‐related proteins in esophageal squamous cell carcinoma using proteomic analysis
Authors: Liu Dong‐Ping , Qi Robert Z. , Wang Yan , Chen Ping‐Ping , Koeffler H. Phillip , Xie Dong 
Published: March 2007

AbstractEsophageal squamous cell carcinoma (ESCC) is the major subtype of esophageal cancers in China, and characterized with high morbidity and mortality. So far, the diagnosis of ESCC is mainly dependent on the alterations in esophageal histology, but most cases of ESCC with low stage do not display visible histological abnormalities. Therefore, a deep understanding of the mechanism of ESCC progression and seeking stage‐specific molecules might improve the diagnosis and therapy for ESCC. In this study, we used proteomics to analyze ESCC tissues with classification by TNM stage, and determined the proteomic features correlated with ESCC progression (from stages I to III). Proteins that exhibited significantly different expression patterns between ESCC and corresponding normal esophageal tissues were identified using MS. The identified proteins with differentiated expression mainly fell into three protein categories (i.e. cytoskeleton system‐associated proteins, metabolism enzymes, and heat shock proteins). In addition, real‐time PCR highlighted some molecules that were associated with tumor stages at the mRNA level, such as enolase 1, chromosome 1 ORF 10, elastase inhibitor, α B crystalline, stress‐induced phosphoprotein 1, and squamous cell carcinoma antigen 1. Altogether, these data provided further information on ESCC progression and potential drug targets for ESCC clinical therapy.
Luminescence and scintillation properties of Y3Al5O12:Pr single crystal
Authors: Mihóková Eva , Nikl Martin , Pejchal Jan , Baccaro Stefania , Cecilia Angelica , Nejezchleb Karel , Vedda Anna 
Published: March 2007

AbstractWe investigated Czochralski‐grown YAG:Pr single crystal as a potential novel scintillator. Optical characteristics including photoluminescence, radioluminescence, scintillation decay, radiation damage and thermoluminescence were measured. Their dependence on the dopant concentration was studied. The radiation damage results show that with increasing concentration of Pr the radiation damage reduces. Thermoluminescence above room temperature shows the dominant glow curve peak around 250 °C. Comparing to YAG:Ce scintillator an advantage of YAG:Pr is its much faster scintillation decay (16‐17 ns at room temperature) and an absence of slow components in the decay. Therefore YAG:Pr appears as potentially interesting scintillator material. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
EPR and optical spectroscopy of MnO42– doped proper ferroelastic K3Na(CrO4)2: local transition and Jahn‐Teller effect
Authors: Maksimova T. , Vikhnin V. , Asatryan H. , Hermanowicz K. , Maczka M. , Hanuza J. 
Published: March 2007

AbstractWe report the study of the polarized optical absorption, the near‐infrared luminescence and the luminescence excitation spectra of the proper ferroelastic K3Na(CrO4)2 crystals doped with MnO42– ions. It is obtained from the luminescence and the d‐d absorption spectra that the 3d1 electronic ground (2E) and excited (2T2) states of MnO42– are split by the monoclinic crystal field of the host lattice at T < 230 K. The well‐resolved vibronic progressions in the luminescence spectra are ascribed to a strong coupling with a MnO42– bending ν2 = 335 cm–1 mode. The spectral dependence of the linear polarization degree ρ (ω) of the charge‐tranfer absorption for E ‖ c and E ‖ b polarization reveals a clear periodic structure due to interaction of local electronic states with a ν1 = 816 cm−1 local mode. The first EPR spectrum of the Mn6+ paramagnetic center in the K3Na(CrO4)2: MnO42– crystal is studied. The local transition due to a local configuration instability of vibronic origin at low temperature T ≤ 18 K is detected and identified. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Polaron conductivity of the strongly defective ZnCr2–xNixSe4 spinels
Authors: Duda H. , Groń T. , Jendrzejewska I. , Mazur S. , Zajdel P. , Kita A. 
Published: March 2007

AbstractElectrical resistivity and the thermoelectric power measurements as well as the calculations of the vacancy parameter, performed in the paramagnetic single crystals of the ZnCr2–xNixSe4 system, provide evidence for polaron conduction in defective spinel materials. At high temperatures a linear dependence between thermopower S and the electrical resistivity (ln ρ), a characteristic of small polarons, is observed. An origin of the small polarons in this case could be associated with a crystalline distortion as the single crystals under study are characterized both by the higher values of the anion parameter than u = 0.375, which is typical for the ideal spinel structure and a large defectiveness, which is identified by the large value of the vacancy parameter of about β = 11.5%. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Extended positron‐trapping defects in insulating MgAl2O4 spinel‐type ceramics
Authors: Klym H. , Ingram A. , Shpotyuk O. , Filipecki J. , Hadzaman I. 
Published: March 2007

AbstractExtended defects in insulating nanoporous MgAl2O4 spinel‐type ceramics which trap positrons are characterized by positron annihilation lifetime spectroscopy before and after water‐immersion treatment. It is shown that in both cases the same type of positron traps prevails, but positron trapping in defects near grain boundaries occurs more efficiently in water‐moistened ceramics. It is shown the more perfect structure of ceramics, the more considerable changes occur in the positron trapping parameters of water‐moistened ceramics. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Europium‐doped barium halide X‐ray scintillators
Authors: Selling J. , Birowosuto M. D. , Dorenbos P. , Schweizer S. 
Published: March 2007

AbstractSingle crystals of europium‐doped barium chloride, bromide, and iodide were investigated under x‐ray and γ‐ray excitation. The Eu2+‐related x‐ray excited luminescence is at 402, 404, and 425 nm for the chloride, bromide, and iodide, respectively. BaCl2:Eu2+has the most promising scintillation properties of the systems investigated. The light yield is about 20,000 ± 2,000 photons per MeV of absorbed γ‐ray energy, the energy resolution for the 662 keV photopeak is 9% ± 1%, and the scintillation decay time is (390 ± 40) ns. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Photoluminescence of Ho3+:YVO4 crystals
Authors: Polosan S. , Bettinelli M. , Tsuboi T. 
Published: March 2007

AbstractEmission spectra of Ho3+‐doped YVO4 crystals are studied in the visible and infrared spectral regions. Besides the emission bands at 544 nm and at about 1950 and 2017 nm due to Ho3+, we observed a broad emission band at 610 nm at low temperatures like 15 K in the highly doped crystals with the Ho3+ concentrations of more than 0.5 mol%. The excitation band for this broad emission is located at 390 nm which is near the edge of band gap of the crystal. It is suggested that the 610 nm band is attributed to defects related to oxygen vacancies around V4+ ions. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Study of Mn2+ luminescence in Zn(PO3)2 glasses
Authors: Flores J. M. C. , Caldino G. U. , Hernández A. J. , Camarillo G. E. , Cabrera B. E. , del Castillo H. , Speghini A. , Bettinelli M. , Murrieta S. H. 
Published: March 2007

AbstractThe optical properties of Mn2+ doped Zn(PO3)2 glass are discussed. Depending on the manganese concentration in the glass samples, the Mn2+ emission band appears in the wavelength range from 515 to 620 nm. This behavior depends on the possible coordination number, a result confirmed by different lifetimes and also different structure of the absorption spectra. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Threshold displacement energy in yttria‐stabilized zirconia
Authors: Costantini Jean‐Marc , Beuneu François 
Published: March 2007

AbstractWe have studied the color center production in yttria‐stabilized zirconia single crystals (i.e. ZrO2: Y with 9.5 mol% Y2O3) with the (100) orientation, by electron irradiations at energies ranging between 1.0 and 2.5 MeV. Electron paramagnetic resonance (EPR) measurements show that three paramagnetic centers are stable at room‐temperature: i) an F+‐type center (singly ionized oxygen vacancy), ii) the so‐called T‐center (Zr3+ in a trigonal oxygen environment), and iii) a hole center. The growth curves of F+‐type centers at variable electron energy cannot be rescaled versus the absorbed dose. These data confirm that F+‐type center production is primarily governed by elastic collision processes with a threshold electron energy about 1.0 MeV. This can be correlated to a threshold displacement energy either about 120 eV for the O‐atoms in the collision cascades, or about 80 eV for the Zr‐ (or Y‐) atoms. In contrast, the growth curves of T‐centers at variable electron energy are rescaled versus the absorbed dose. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
EPR of Cu2+ in lithium tetraborate single crystals
Authors: Corradi G. , Watterich A. , Polgár K. , Nagirnyi V. , Hofstaetter A. , Rakitina L. G. , Meyer M. 
Published: March 2007

AbstractA low temperature electron paramagnetic resonance study of single crystals of the scintillator and thermoluminescent dosimeter material lithium tetraborate (Li2B4O7) doped by Cu has been carried out. The observed spectra of an unpaired hole with strong d ‐character and characteristic hyperfine splittings can be ascribed to Cu2+ situated at or near the Li lattice site, which supports the conclusions of a parallel luminescence study about the low symmetry of emitting Cu+ sites. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The thermostimulated exoelectron emission of NaF:U,Me compounds after electron beam irradiation
Authors: Kidibaev M. M. , Dzholdoshov B. K. , Koroleva T. S. , Slesarev A. I. , Shulgin B. V. , Ivanov V. Yu. , Tcherepanov A. N. , Pedrini Ch. , Lebbou K. 
Published: March 2007

AbstractThe thermostimulated exoelectron emission (TSEE) of NaF:(U,Me) compounds after electron beam irradiation in the 300–800 K temperature range has been investigated. For NaF:(U,Me) bulk, fiber and nanocrystals several regular peaks of TSEE have been found. Additional effects with the sign of thermostimulated explosive electron emission have been found too. The nature of TSEE process is discussed. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
VUV 5d – 4f luminescence of Gd3+ doped into CaF2
Authors: Makhov V. N. , Batygov S. Kh. , Dmitruk L. N. , Kirm M. , Stryganyuk G. , Zimmerer G. 
Published: March 2007

AbstractHigh‐resolution (∼1 Å) emission and excitation spectra as well as luminescence decay kinetics have been studied for CaF2 doped with Gd3+(0.1%) and Ce3+(0.05%) under excitation by synchrotron radiation near and above the edge of interconfigurational 4f7 – 4f65d transitions in Gd3+ and 4f – 5d transitions in Ce3+. The fast (τ ∼ 8.5 ns) VUV luminescence has been detected from the crystal the spectrum of which shows zero‐phonon line at 77660 cm–1 and dominating vibronic line at energy interval ∼370 cm–1. The vibronic structure in VUV emission spectrum well correlates with that observed at the onset of the Gd3+ 4f7 – 4f65d excitation spectrum. The obtained results confirm that the VUV luminescence observed in CaF2:Gd3+,Ce3+ originates from 4f65d – 4f7 radiative transitions in Gd3+. The vibronic structure in emission and excitation spectra of Gd3+ d – f luminescence from the CaF2:Gd3+,Ce3+ crystal differs from that observed for other trivalent rare earth ions doped into CaF2 indicating that Gd3+ ions reside in sites of different type in CaF2 than that of tetragonal (C4v) symmetry. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Neutron, ion and electron induced defects in activated LiF and NaF single crystals
Authors: Shulgin B. V. , Ivanov V. Yu. , Tcherepanov A. N. , Petrov V. L. , Anipko A. V. , Neshov F. G. , Kidibaev M. M. , Koroleva T. S. , Solomonov V. I. , Kaigorodova O. A. 
Published: March 2007

AbstractThe neutron induced defects in activated LiF and NaF single crystals have been investigated by studying the absorption and luminescence spectra. For LiF and NaF neutron irradiated crystals the creation of F, F2 and F3+ aggregate color centers have been analysed in comparison with F, F2 and F3+‐center production in NaF crystals irradiated by ion and electron beams. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)