bluuebunny/crossref_metadata_2025_split huggingface dataset:
RBM22 regulates RNA polymerase II 5′ pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5
Authors: Du Xian , Qin Wenying , Yang Chunyu , Dai Lin , San Mingkui , Xia Yingdan , Zhou Siyu , Wang Mengyang , Wu Shuang , Zhang Shaorui , Zhou Huiting , Li Fangshu , He Fang , Tang Jingfeng , Chen Jia-Yu , Zhou Yu , Xiao Rui 
Published: April 2024

Abstract

Background

Splicing factors are vital for the regulation of RNA splicing, but some have also been implicated in regulating transcription. The underlying molecular mechanisms of their involvement in transcriptional processes remain poorly understood.

Results
Here, we describe a direct role of splicing factor RBM22 in coordinating multiple steps of RNA Polymerase II (RNAPII) transcription in human cells. The RBM22 protein widely occupies the RNAPII-transcribed gene locus in the nucleus. Loss of RBM22 promotes RNAPII pause release, reduces elongation velocity, and provokes transcriptional readthrough genome-wide, coupled with production of transcripts containing sequences from downstream of the gene. RBM22 preferentially binds to the hyperphosphorylated, transcriptionally engaged RNAPII and coordinates its dynamics by regulating the homeostasis of the 7SK-P-TEFb complex and the association between RNAPII and SPT5 at the chromatin level.

Conclusions
Our results uncover the multifaceted role of RBM22 in orchestrating the transcriptional program of RNAPII and provide evidence implicating a splicing factor in both RNAPII elongation kinetics and termination control.
Single Cell Atlas: a single-cell multi-omics human cell encyclopedia
Authors: Pan Lu , Parini Paolo , Tremmel Roman , Loscalzo Joseph , Lauschke Volker M. , Maron Bradley A. , Paci Paola , Ernberg Ingemar , Tan Nguan Soon , Liao Zehuan , Yin Weiyao , Rengarajan Sundararaman , Li Xuexin ,   The SCA Consortium
Published: April 2024

AbstractSingle-cell sequencing datasets are key in biology and medicine for unraveling insights into heterogeneous cell populations with unprecedented resolution. Here, we construct a single-cell multi-omics map of human tissues through in-depth characterizations of datasets from five single-cell omics, spatial transcriptomics, and two bulk omics across 125 healthy adult and fetal tissues. We construct its complement web-based platform, the Single Cell Atlas (SCA,www.singlecellatlas.org), to enable vast interactive data exploration of deep multi-omics signatures across human fetal and adult tissues. The atlas resources and database queries aspire to serve as a one-stop, comprehensive, and time-effective resource for various omics studies.
A maturity model for the scientific review of clinical trial designs and their informativeness
Authors: Dolley S , Norman T , McNair D , Hartman D 
Published: April 2024

Abstract
Background
Informativeness, in the context of clinical trials, defines whether a study’s results definitively answer its research questions with meaningful next steps. Many clinical trials end uninformatively. Clinical trial protocols are required to go through reviews in regulatory and ethical domains: areas that focus on specifics outside of trial design, biostatistics, and research methods. Private foundations and government funders rarely require focused scientific design reviews for these areas. There are no documented standards and processes, or even best practices, toward a capability for funders to perform scientific design reviews after their peer review process prior to a funding commitment.

Main body
Considering the investment in and standardization of ethical and regulatory reviews, and the prevalence of studies never finishing or failing to provide definitive results, it may be that scientific reviews of trial designs with a focus on informativeness offer the best chance for improved outcomes and return-on-investment in clinical trials. A maturity model is a helpful tool for knowledge transfer to help grow capabilities in a new area or for those looking to perform a self-assessment in an existing area. Such a model is offered for scientific design reviews of clinical trial protocols. This maturity model includes 11 process areas and 5 maturity levels. Each of the 55 process area levels is populated with descriptions on a continuum toward an optimal state to improve trial protocols in the areas of risk of failure or uninformativeness.

Conclusion
This tool allows for prescriptive guidance on next investments to improve attributes of post-funding reviews of trials, with a focus on informativeness. Traditional pre-funding peer review has limited capacity for trial design review, especially for detailed biostatistical and methodological review. Select non-industry funders have begun to explore or invest in post-funding review programs of grantee protocols, based on exemplars of such programs. Funders with a desire to meet fiduciary responsibilities and mission goals can use the described model to enhance efforts supporting trial participant commitment and faster cures.
Haem iron versus ferrous iron salts to treat iron deficiency anaemia in Gambian children: protocol for randomised controlled trial {1}
Authors: Bah Mamadou , Verhoef Hans , Okoh Emmanuel , Bah Abdoulie , Prentice Andrew M. , Cerami Carla 
Published: April 2024

Abstract
Background
The World Health Organization recommends universal iron supplementation for children aged 6–23 months in countries where anaemia is seen in over 40% of the population. Conventional ferrous salts have low efficacy due to low oral absorption in children with inflammation. Haem iron is more bioavailable, and its absorption may not be decreased by inflammation. This study aims to compare daily supplementation with haem iron versus ferrous sulphate on haemoglobin concentration and serum ferritin concentration after 12 weeks of supplementation.

Methods
This will be a two-arm, randomised controlled trial. Gambian children aged 6–12 months with anaemia will be recruited within a predefined geographical area and recruited by trained field workers. Eligible participants will be individually randomised using a 1:1 ratio within permuted blocks to daily supplementation for 12 weeks with either 10.0 mg of elemental iron as haem or ferrous sulphate. Safety outcomes such as diarrhoea and infection-related adverse events will be assessed daily by the clinical team (see Bah et al. Additional file 4_Adverse event eCRF). Linear regression will be used to analyse continuous outcomes, with log transformation to normalise residuals as needed. Binary outcomes will be analysed by binomial regression or logistic regression, Primary analysis will be by modified intention-to-treat (i.e., those randomised and who ingested at least one supplement dose of iron), with multiple imputations to replace missing data. Effect estimates will be adjusted for baseline covariates (C-reactive protein, alpha-1-acid glycoprotein, haemoglobin, ferritin, soluble transferrin receptor).

Discussion
This study will determine if therapeutic supplementation with haem iron is more efficacious than with conventional ferrous sulphate in enhancing haemoglobin and ferritin concentrations in anaemic children aged 6–12 months.

Trial registration
Pan African Clinical Trial Registry PACTR202210523178727
Renal artery pseudoaneurysm following robot assisted nephron sparing surgery: two case reports
Authors: Mavuduru Ravi Mohan , Mohd Ziauddin Shiraz Akif , Bora Girdhar Singh , Gorsi Ujjwal 
Published: April 2024

Abstract
Background
Renal artery pseudoaneurysm following partial nephrectomy is a rare entity, the incidence of this entity is more common following penetrating abdominal injuries, percutaneous renal interventions such as percutaneous nephrostomy(PCN) or Percutaneous nephrolithotomy (PCNL). Although rare, renal artery pseudoaneurysm can be life threatening if not managed timely, they usually present within two weeks postoperatively with usual presenting complains being gross haematuria, flank pain and/or anaemia.

Case presentation
We report case of two female patients 34 and 57 year old respectively of South Asian ethnicity, presenting with renal artery pseudoaneurysm following left sided robot assisted nephron sparing surgery for interpolar masses presenting clinically with total, painless, gross haematuria with clots within fifteen days postoperatively and their successful treatment by digital subtraction angiography and coil embolization.

Conclusion
Renal artery aneurysm is a rare fatal complication of minimally invasive nephron sparing surgery however considering the preoperative and intraoperative risk factors for its development and prompt suspicion at the outset can be life saving with coil embolization of the bleeding arterial aneurysm.
Reconstruction of the solution of inverse Sturm–Liouville problem
Authors: Wei Zhaoying , Hu Zhijie , Xiang Yuewen 
Published: April 2024

AbstractIn this paper we are concerned with an inverse problem with Robin boundary conditions, which states that, when the potential on $[0,1/2]$
[
0
,
1
/
2
]
and the coefficient at the left end point are known a priori, a full spectrum uniquely determines its potential on the whole interval and the coefficient at the right end point. We shall give a new method for reconstructing the potential for this problem in terms of the Mittag-Leffler decomposition of entire functions associated with this problem. The new reconstructing method also deduces a necessary and sufficient condition for the existence issue.
Ten years of hand hygiene excellence: a summary of outcomes, and a comparison of indicators, from award-winning hospitals worldwide
Authors: Tartari Ermira , Garlasco Jacopo , Mezerville Marcela Hernández-de , Ling Moi Lin , Márquez-Villarreal Hilda , Seto Wing-Hong , Simon Anne , Hennig Thomas-Jörg , Pittet Didier 
Published: April 2024

Abstract
Background
Hand hygiene is a crucial measure for the prevention of healthcare-associated infections (HAIs). The Hand Hygiene Excellence Award (HHEA) is an international programme acknowledging healthcare facilities for their leadership in implementing hand hygiene improvement programmes, including the World Health Organisation’s Multimodal Improvement Strategy. This study aimed at summarising the results of the HHEA campaign between 2010 and 2021 and investigating the relationship between different hand hygiene parameters based on data from participating healthcare facilities.

Methods
A retrospective analysis was performed on datasets from HHEA forms, including data on hand hygiene compliance, alcohol-based handrub (ABHR) consumption, and Hand Hygiene Self-Assessment Framework (HHSAF) scores. Descriptive statistics were reported for each variable. The correlation between variables was inspected through Kendall’s test, while possible non-linear relationships between hand hygiene compliance, ABHR consumption and HHSAF scores were sought through the Locally Estimated Scatterplot Smoothing or logistic regression models. A tree-structured partitioning model was developed to further confirm the obtained findings.

Results
Ninety-seven healthcare facilities from 28 countries in three world regions (Asia-Pacific, Europe, Latin America) were awarded the HHEA and thus included in the analysis. HHSAF scores indicated an advanced hand hygiene promotion level (median 445 points, IQR 395–480). System change (100 [95–100] points) and institutional safety climate (85 [70–95] points) showed the highest and lowest score, respectively. In most cases, hand hygiene compliance was above 70%, with heterogeneity between countries. ABHR consumption above 20 millilitres per patient-day (ml/PD) was widely reported, with overall increasing trends. HHSAF scores were positively correlated with hand hygiene compliance (τ = 0.211, p = 0.007). We observed a positive correlation between compliance rates and ABHR consumption (τ = 0.193, p < 0.001), although the average predicted consumption was stable around 55–60 ml/PD for compliance rates above 80–85%. Logistic regression and partitioning tree analyses revealed that higher HHSAF scores were more likely in the high-ABHR consumption group at cut-offs around 57–59 ml/PD.

Conclusion
Ten years after its inception, the HHEA proves to be a valuable hand hygiene improvement programme in healthcare facilities worldwide. Consistent results were provided by the different hand hygiene indicators and the HHSAF score represents a valuable proxy measure of hand hygiene compliance.
Person-centered care assessment tool with a focus on quality healthcare: a systematic review of psychometric properties
Authors: Bru-Luna Lluna Maria , Martí-Vilar Manuel , Merino-Soto César , Livia-Segovia José , Garduño-Espinosa Juan , Toledano-Toledano Filiberto 
Published: April 2024

AbstractBackgroundThe person-centered care (PCC) approach plays a fundamental role in ensuring quality healthcare. The Person-Centered Care Assessment Tool (P-CAT) is one of the shortest and simplest tools currently available for measuring PCC. The objective of this study was to conduct a systematic review of the evidence in validation studies of the P-CAT, taking the “Standards” as a frame of reference.MethodsFirst, a systematic literature review was conducted following the PRISMA method. Second, a systematic descriptive literature review of validity tests was conducted following the “Standards” framework. The search strategy and information sources were obtained from the Cochrane, Web of Science (WoS), Scopus and PubMed databases. With regard to the eligibility criteria and selection process, a protocol was registered in PROSPERO (CRD42022335866), and articles had to meet criteria for inclusion in the systematic review.ResultsA total of seven articles were included. Empirical evidence indicates that these validations offer a high number of sources related to test content, internal structure for dimensionality and internal consistency. A moderate number of sources pertain to internal structure in terms of test-retest reliability and the relationship with other variables. There is little evidence of response processes, internal structure in measurement invariance terms, and test consequences.DiscussionThe various validations of the P-CAT are not framed in a structured, valid, theory-based procedural framework like the “Standards” are. This can affect clinical practice because people’s health may depend on it. The findings of this study show that validation studies continue to focus on the types of validity traditionally studied and overlook interpretation of the scores in terms of their intended use.
Comparison of therapeutic effects of N-Acetylcysteine with pregabalin in improving the clinical symptoms of painful diabetic neuropathy: a randomized, double-blind clinical trial
Authors: Sajedi Firozeh , Abdi Arman , Mehrpooya Maryam , Faramarzi Vida , Mohammadi Younes , Sheida Fateme 
Published: April 2024

Abstract
Objectives
Painful diabetic neuropathy (PDN) is highly prevalent and annoyingly in patients with diabetes. The aim of this study was to investigate the effects of oral N-acetylcysteine (NAC) compared to pregabalin in PDN.

Methods
One hundred two eligible patients with type 2 diabetes and PDN were randomly recievied pregabalin (150 mg/day) or N-Acetylcysteine (NAC) (600 mg/ twice a day) for 8 weeks. Mean pain score, Sleep interference score (SIS), Patient Global Impression of Change (PGIC), Clinical Global Impression of Change (CGIC), and also, serum levels of total antioxidant capacity (TAC), total thiol groups (TTG), catalase activity (CAT), nitric oxide (NO), and malondialdehyde (MDA) were assessed at baseline and at the end of the study.

Results
NAC was well tolerated in all patients. The decrease in mean pain scores and increase in SIS was similar between two groups. More improvement in PGIC and CGIC from the baseline was reported in NAC group. NAC, significantly, decreased serum levels of MDA, and NO, but increased TAC, TTG, and CAT. Pregabalin, significantly, decreased serum levels of MDA, and NO and increased TAC.

Discussion
NAC is efficacious in alleviate symptoms of PDN which is probably related to its antioxidant effects.

Trial registration
The research protocol received approval from the Ethics Committee of Hamadan University of Medical Sciences (IR.UMSHA.REC.1397.137). The trial registry URL and number in Iranian Registry of Clinical Trials (IRCT): https://www.irct.ir/trial/33313, IRCT20180814040795N2 (Registration date: 2019-01-21, Retrospectively registered)
Where scrollbars are clicked, and why
Authors: Herbort Oliver , Raßbach Philipp , Kunde Wilfried 
Published: April 2024

AbstractScrolling is a widely used mean to interact with visual displays, usually to move content to a certain target location on the display. Understanding how user scroll might identify potentially suboptimal use and allows to infer users’ intentions. In the present study, we examined where users click on a scrollbar depending on the intended scrolling action. In two online experiments, click positions were systematically adapted to the intended scrolling action. Click position selection could not be explained as strict optimization of the distance traveled with the cursor, memory load, or motor-cognitive factors. By contrast, for identical scrolling actions click positions strongly depended on the context and on previous scrolls. The behavior of our participants closely resembled behavior observed for manipulation of other physical devices and suggested a simple heuristic of movement planning. The results have implications for modeling human–computer interaction and may contribute to predicting user behavior.
Sex and stature estimation from anthropometric measurements of the foot: linear analyses and neural network approach on a Turkish sample
Authors: Parlak Muhammed Emin , Özkul Bengü Berrak , Oruç Mucahit , Celbiş Osman 
Published: April 2024

Abstract
Background
For over a century, anthropometric techniques, widely used by anthropologists and adopted by medical scientists, have been utilized for predicting stature and sex. This study, conducted on a Eastern Turkish sample, aims to predict sex and stature using foot measurements through linear methods and Artificial Neural Networks. Our research was conducted on 134 medical students, comprising 69 males and 65 females. Stature and weight were measured in a standard anatomical position in the Frankfurt Horizontal Plane with a stadiometer of 0.1 cm precision. Measurements of both feet's height, length, and breadth were taken using a Vernier caliper, osteometric board, and height scale. The data were analyzed using SPSS 26.00.

Results
It was observed that all foot dimensions in males were significantly larger than in females. Sex prediction using linear methods yielded an accuracy of 94.8%, with a stature estimation error of 4.15 cm. When employing Artificial Neural Networks, sex prediction accuracy increased to 97.8%, and the error in stature estimation was reduced to 4.07 cm.

Conclusions
Our findings indicate that Artificial Neural Networks can work more effectively with such data. Using Artificial Neural Networks, the accuracy of sex prediction for both feet exceeded 95%. Additionally, the error in stature estimation was reduced compared to the formulas obtained through linear methods.
Embolisation of a high – flow renal arteriovenous fistula with the use of simultaneous transvenous and transarterial approach and balloon-assisted coil embolization
Authors: Markoutsas D. , Tzavoulis D. , Tsoukalos G. , Ioannidis I. 
Published: April 2024

Abstract
Background
Renal arteriovenous fistula (RAVF) is a rare vascular malformation, which can be asymptomatic or may cause hemorrhage, hypokalaemic hypertension, heart failure and hematuria. Endovascular embolization is a minimally invasive method which can preserve renal parenchyma. In our case, balloon assisted coil embolization with simultaneous transvenous and transarterial approach was used. A remodelling balloon, which is routinely used in neurovascular procedures, was chosen in order to eliminate the risk of coil migration and preserve feeding artery and renal parenchyma.

Case presentation
We present a case of successful balloon – assisted coil embolization of a high flow renal arteriovenous fistula in a 25-year-old male patient via simultaneous transarterial and transvenous approach with preservation of the feeding artery.

Conclusion
Endovascular embolisation is a safe and effective treatment of RAVFs with low risk of complications. Simultaneous transarterial and transvenous coil deployment with the use of a flow control balloon catheter can eliminate the risk of coil migration and coil protrusion into the parent artery with permanent RAVF occlusion and renal parenchyma preservation.
Approaches to sustaining people–place bonds in conservation planning: from value-based, living heritage, to the glocal community
Authors: Chen Yueyi , Wang Yi-Wen 
Published: April 2024

AbstractThis review paper explores approaches to sustaining the enduring connection between communities and heritage places in conservation planning. Amidst global overtourism, the gradual outmigration and sudden displacement of local communities from heritage places disrupt not only physical ties between people and places but also shared traditions, memories and emotional bonds. These intangible qualities constitute the social dimension of heritage and are pivotal to its cultural significance. However, existing conservation approaches to safeguarding this social dimension often rely on the continued presence of local communities, which may yield limited effectiveness in restoring people–place bonds amidst challenges posed by overtourism and displacement. This review unpacks the term ‘social value’ within the framework of value-based assessment and progresses towards a dynamic conception of ‘sense of place’. In response to the changing social composition of ‘local communities’ at heritage places, this review examines approaches for conserving the social dimension of heritage, aiming to sustain the enduring continuity of people–place bonding. It proposes a broader definition of ‘sense of place’ that transcends the preconceived notion of stakeholder groups as a geographically defined community and suggests leveraging digital social platforms to re-establish these bonds. The review concludes by advocating for the ‘glocal community’ approach, acknowledging the personal emotional connections that individuals form with a heritage place regardless of their places of residence. This approach has the potential to complement existing methods, such as value-based assessment and the living heritage approach, which are commonly practised in contemporary conservation efforts.