bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wound repair and proliferation of bronchial epithelial cells regulated by CTNNAL1
Authors: Xiang Yang , Tan Yu‐Rong , Zhang Jian‐Song , Qin Xiao‐Qun , Hu Bi‐Bo , Wang Yue , Qu Fei , Liu Hui‐Jun 
Published: July 2007

AbstractAdhesion molecules play vital roles in airway hyperresponsiveness (AHR) or airway inflammation. Our previous study indicated that adhesion molecule catenin alpha‐like 1 (CTNNAL1) is relevant closely to asthma susceptibility, but its biological function or significance is still unclear. In the present study, we observed the temporal and spatial distribution of CTNNAL1 expression in mouse lung tissue with the OVA‐sensitized asthma model and found that the level of CTNNAL1 mRNA showed a prominent negative correlation with pulmonary resistance (RL). To study the function of CTNNAL1 in airway, effects of CTNNAL1 on proliferation and wound repair activity of human bronchial epithelial cells (HBEC) was investigated with antisense oligonucleotide (ASO) technique. The results showed that: (1) CTNNAL1 ASO could decelerate the repairing velocity and proliferation of HBEC; (2) CTNNAL1 expression was increased on the edge cells of mechanic wounded area in culture; (3) extracellular matrix component fibronectin (Fn) obviously promoted wound repair activity and proliferation of HBEC, which could be blocked by CTNNAL1 ASO; (4) Western blot showed that Fn could promote FAK phosphorylation, which also be inhibited by CTNNAL1 ASO. In conclusion, the level of CTNNAL1 mRNA expression is highly correlated to airway resistance; CTNNAL1 may contribute to the wound repair and proliferation of HBEC. Furthermore, it may serve to Fn mediated cell‐extracellular adhesion and its signal transduction. J. Cell. Biochem. 103: 920–930, 2008. © 2007 Wiley‐Liss, Inc.
Secretion of atherogenic risk factor apolipoprotein B‐100 is increased by a potential mechanism of JNK/PKC‐mediated insulin resistance in liver cells
Authors: Jin Un‐Ho , Kang Yun‐Jeong , Chang Young‐Chae , Kim Cheorl‐Ho 
Published: July 2007

AbstractApolipoprotein B‐100 (ApoB) is the main protein of the atherogenic lipoproteins and plasma ApoB levels reflect the total numbers of atherogenic lipoproteins. Induction of insulin resistance was accompanied by a considerable rise in the production of hepatic very low density lipoprotein (VLDL) containing ApoB and triglyceride. Increased plasma levels of ApoB and triglyceride in VLDL are common characteristics of the dyslipidemia associated with insulin resistance and type 2 diabetes mellitus. Thus, we investigate whether phorbol 12‐myristate‐13‐acetate (PMA)‐induced insulin resistance affects the increase of ApoB secretion. PMA increased ApoB secretion and transcriptional level of microsomal triglyceride transfer protein (MTP). PMA treatment also resulted in increase of insulin receptor substrate 1 (IRS1) serine312 (Ser312) and serine1101 (Ser1101) phosphorylation and induction of IRS1 degradation. Additionally, PMA induced activation of c‐jun N‐terminal kinase (JNK) and protein kinase C (PKC) isoforms (α, βI, δ, ζ, θ), and reduced AKT8 virus oncogene cellular homolog (AKT) activation in a time dependent manner. PMA‐induced ApoB secretion, MTP promoter activities, and IRS1 degradation was significantly decreased by treatment of JNK and PKCs inhibitors. Orthovanadate, a potent tyrosine phosphatase inhibitor, increased tyrosine phosphorylation of IRS1 and decreased ApoB secretion of Chang liver cells although PMA was co‐treated. From the results, it was concluded that PMA‐induced insulin resistance, through induction of serine phosphorylation of IRS1 mediated by activated JNK and PKCs, increases ApoB secretion in Chang liver cells. J. Cell. Biochem. 103: 908–919, 2008. © 2007 Wiley‐Liss, Inc.
Some thoughts about translational regulation: Forward and backward glances
Authors: Kozak Marilyn 
Published: July 2007

AbstractThis review discusses the need to re‐examine some popular but unproven ideas about regulation of translation in eukaryotes. Translational control is invoked often on superficial grounds, such as a discrepancy between mRNA and protein levels which could be explained instead by rapid turnover of the protein. It is essential to verify that there is translational control (i.e., essential to rule out alternative mechanisms) before asking how translation is regulated. Many of the postulated control mechanisms are dubious. It is easy to create artifactual regulation (a slight increase or decrease in translation) by over‐expressing recombinant RNA‐binding proteins. The internal‐initiation hypothesis is the source of other misunderstandings. Recent claims about the involvement of internal ribosome entry sequences (IRESs) in cancer and other diseases are discussed. The scanning model for initiation provides a more credible framework for understanding many aspects of translation, including ways to restrict the production of potent regulatory proteins which would be harmful if over‐expressed. The rare production in eukaryotes of dicistronic mRNAs (e.g., from retrotransposons) raises questions about how the 3′ cistron gets translated. Some proposed mechanisms are discussed, but the available evidence does not allow resolution of the issue. J. Cell. Biochem. 102: 280–290, 2007. © 2007 Wiley‐Liss, Inc.
Characterization of Apin, a secreted protein highly expressed in tooth‐associated epithelia
Authors: Moffatt Pierre , Smith Charles E. , St‐Arnaud René , Nanci Antonio 
Published: July 2007

AbstractWe previously reported expression of a protein by enamel organ (EO) cells in rat incisors, originally isolated from the amyloid of Pindborg odontogenic tumors called Apin. The aim of the present study was to further characterize the Apin gene and its protein in various species, assess tissue specificity, and clarify its localization within the EO. Northern blotting and RT‐PCR revealed that expression of Apin was highest in the EO and gingiva, moderate in nasal and salivary glands, and lowest in the epididymis. The protein sequences deduced from the cloned cDNA for rat, mouse, pig, and human were aligned together with those obtained from four other mammal genomes. Apin is highly conserved in mammals but is absent in fish, birds, and amphibians. Comparative SDS–PAGE analyses of the protein obtained from bacteria, transfected cells, and extracted from EOs all indicated that Apin is post‐translationally modified, a finding consistent with the presence of predicted sites for phosphorylation and O‐linked glycosylation. In rodent incisors, Apin was detected only in the ameloblast layer of the EO, starting at post‐secretory transition and extending throughout the maturation stage. Intense labeling was visible over the Golgi region as well as on the apices of ameloblasts abutting the enamel matrix. Apin was also immunodetected in epithelial cells of the gingiva which bind it to the tooth surface (junctional epithelium). The presence of Apin at cell‐tooth interfaces suggests involvement in adhesive mechanisms active at these sites, but its presence among other epithelial tissues indicates Apin likely possesses broader physiological roles. J. Cell. Biochem. 103: 941–956, 2008. © 2007 Wiley‐Liss, Inc.
Energetic trade‐offs between immunity and reproduction in male japanese quail (Coturnix coturnix)
Authors: Boughton Raoul K. , Bridge Eli S. , Schoech Stephan J. 
Published: July 2007

AbstractWe investigated a postulated trade‐off between reproduction and immune function by comparing the energetic costs of an immune response with phytohemagglutinin challenge (or injection) in castrated (low testosterone [T]) and intact (high T) Japanese Quail (Coturnix coturnix). Intact birds had higher resting metabolic rate (RMR) and significantly lower immune response than castrates. RMR of intact birds did not change in response to an immune challenge, suggesting that maintenance of reproductive tissues and associated high T is both immunosuppressive and energetically costly. Despite having a greater immune response than intact quail, castrates had a lower pre‐challenge RMR than intact birds and paradoxically tended to decrease RMR during an immune challenge. This paradox may be because of pro‐inflammatory cytokines that are released during immune responses. Cytokines promote energy conservation through malaise and soporific behaviors, possibly explaining the co‐occurrence of a relatively strong immune response and a decrease in nocturnal RMR in castrates. The lower immune response in intact birds may not elicit as great a response of pro‐inflammatory cytokines owing to an already elevated RMR from reproductive state, thus reducing any effect on RMR. The suppressed immune response and elevated RMR in intact birds may be because of T; however, we cannot separate the effects of T per se from the metabolic requirements of reproductive tissues. J. Exp. Zool. 307A:479–487, 2007. © 2007 Wiley‐Liss, Inc.
Externalization of employment in a service environment: the role of organizational and customer identification
Authors: Johnson Scott A. , Ashforth Blake E. 
Published: July 2007

AbstractThis study investigates the impact of employment externalization (in the form of limited‐term vs. permanent employment status) on customer‐oriented service behavior, and how identification processes may help to resolve the ‘paradox of externalization’ (i.e., organizations relying more on potentially disenfranchised employees to maintain strong connections with their customers). Survey data were obtained from 369 sales, service, and technical support personnel from the Canadian subsidiary of a large international service organization in the high technology sector. Organizational and customer identification fully mediate the relationship between employment status and customer‐oriented service behavior. Additionally, the perceived external image of the organization and the visibility of one's affiliation with the organization moderate the relationships between employment status and organizational and customer identification. Copyright © 2007 John Wiley & Sons, Ltd.
The influence of age on volunteer contributions in a nonprofit organization
Authors: Caldwell Steven D. , Farmer Steven M. , Fedor Donald B. 
Published: July 2007

AbstractThis study examines the role of chronological age and age diversity in relation to the effects of organizational influences on the contributions of 458 volunteers in 74 geographically dispersed teams of a large nonprofit organization. The results indicate that the quality of member selection has a greater positive influence on in‐role performance for older rather than younger volunteers that the quality of training for group members has a greater positive influence on in‐role performance for volunteers who are less dissimilar in age from others in the group, and that the positive effects of chronological age on helping behaviors depend on the mean age of the group such that it is stronger for groups with older age means. The findings are discussed along with their practical implications. Copyright © 2007 John Wiley & Sons, Ltd.
Induced cross‐protection responses against Cr(III) and Fe(III) ions in Saccharomyces cerevisiae
Authors: Fujs Štefan , Ekert Martina , Ščančar Janez , Raspor Peter 
Published: July 2007

AbstractStress tolerance of yeast Saccharomyces cerevisiae was examined after exposure to iron and chromium, which are essential minerals in low concentrations but can be toxic if present in high concentrations. Induction of possible cross‐protection responses was performed with the yeast pre‐treatment at the start of cultivation with low concentrations of Fe(III) or Cr(III) ions, which slightly inhibit the growth and the subsequent exposure to sub‐lethal concentrations of Fe(III) or Cr(III) ions in the mid‐exponential phase. No cross‐protection was found if yeasts were pre‐treated with 0.1 mM Cr(III) and subsequent exposure to 2.5 mM Fe(III) ions took place. If pre‐treated with 0.1 mM Fe(III) Saccharomyces cerevisiae conferred protection to subsequent challenges with a sub‐lethal concentration of 2.5 mM Cr(III) ions resulting in higher biomass formation and higher relative cell viability in comparison to cells without pre‐treatment. It is shown for the first time that iron pre‐treatment enhanced yeast condition against chromium related stress via cross‐protection mechanism. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The effect of soil type and plant age on the population size of rhizospheric methanotrophs and their activities in tropical rice soils
Authors: Vishwakarma Pranjali , Kumar Dubey Suresh 
Published: July 2007

AbstractA laboratory incubation experiment was conducted in tropical rain‐fed (red soil) and irrigated (black soil) rice agroecosystem during the crop growing season to determine the effect of the type of soil, cultivation practices and the age of plant on MOB (methane oxidizing bacteria) population size and their activities. The average value of MOB population size was 11.7 ± 4.5 × 105 cells g–1soil, with a range of 3.1 ± 0.4 to 21.2 ± 1.0 × 105 cells g–1 soil for red soil, which was lower in comparison to black soil where population size varied between 84.2 ± 3.8 and 289.4 ± 7.0 × 105 cells g–1 soil with an average of 182.8 ± 53.5 × 105 cells g–1 soil. The highest population size was recorded during the grain maturation stage which gradually declined during the grain filling, flowering and tillering stages of the rice plants. The HSD test indicated a significant variation in the MOB population size with the varying ages of the plant. CH4 oxidizing capacity was higher in black soil as compared to red soil. The highest CH4 oxidizing capacity was found at the grain‐filling stage in both the soil types. The differences in soil types and cultivation practices, pattern of variation in MOB population size and methane oxidation were found similar in both the sites under the influence of plant age, even though the detected values differed significantly. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Yeasts colonizing the leaf surfaces
Authors: Sláviková Elena , Vadkertiová Renata , Vránová Dana 
Published: July 2007

AbstractThe yeasts were isolated from the leaf surfaces of ten species of trees. The study site was a forest park (Železná Studnička) of the Small Carpathians mountain range. One hundred and thirty seven yeast strains belonging to 13 genera were isolated from 320 samples of leaves and needles. Seventeen yeast species were isolated, but only seven occurred regularly: Aureobasidium pullulans, Cryptococcus laurentii, Pichia anomala, Metschnikowia pulcherrima, Saccharomyces sp., Lachancea thermotolerans, and Rhodotorula glutinis. The remaining species were isolated from the leaves and needles of three or less tree species. A. pullulans, Cr. laurentii, and P. anomala were the most frequently found species and they occurred on leaves and needles of all ten tree species. Saccharomyces sp. occurred in leaf samples collected from eight kinds of trees. M. pulcherrima and L. thermotolerans were found in samples collected from six species of trees. Both these species occurred almost always on the leaves of deciduous trees. Rh. glutinis was the most frequently isolated carotenoids producing species. We have found out that the ascomycetous and basidiomycetous species were present in the leaf samples in approximately equal frequency, contrary to the soil samples taken from this forest park, where the ascomycetous species were found rarely. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Resolving orographic rainfall on the Indian west coast
Authors: Suprit K. , Shankar D. 
Published: July 2007

AbstractWe discuss a method to obtain the spatial estimates of rainfall on the Indian west coast for calculating the surface water budget. The region includes the basin of the Mandovi River, a typical west‐coast river whose catchment area includes the Sahyadris range. The rainfall maps obtained by a multivariate interpolation scheme, with elevation as the third variable, were able to capture the sharp increase in rainfall on the windward slopes and the exponential decay in rainfall on the leeward side. The rainfall maps were used to force a hydrological model and the simulated discharge was within 16% of the observed discharge in 16 of 18 years during 1981–1998; the average error over the 18 years was 8%, which is much less than the estimated error of ∼15–20% in the observed discharge. The Mandovi river discharge provided a reliable and independent validation of the accuracy of the rainfall estimate as the river is purely rain‐fed. The method provides a better estimate of rainfall in the Mandovi basin, and therefore for the Indian west coast, than existing rainfall datasets. The key result is that treating the windward and leeward sides separately by an a priori, subjective extraction of the ridge line can reduce the underestimation of rainfall that is common in mountainous terrain when the data coverage is sparse. Copyright © 2007 Royal Meteorological Society
Kinetics of reaction of copper with substituted benzyl chlorides in dipolar aprotic solvents
Authors: Egorov Anatoly M. , Matyukhova Svetlana A. 
Published: July 2007

AbstractThe reaction of copper with benzyl chlorides in dipolar aprotic solvents has been investigated. The kinetic and thermodynamic parameters of the reaction of copper with benzyl chloride in dimethyl sulfoxide, dimethylacetamide, and hexamethylphosphoramide have been obtained. Hammett plots of log (k/k°) versus the substituent constant σ gave good correlation. The structure of the organic group has little effect on the rate of reaction of benzyl chloride with copper. The kinetic and thermodynamic parameters were correlated with donor number of solvent (DN). The ratio kRBr/kRCl and Hammett ρ values provide evidence in favor of the halogen atom transfer mechanism. © 2007 Wiley Periodicals, Inc. Int J Chem Kinet 39: 547–555, 2007
Kinetics of the CH3O2 + HO2 reaction: A temperature and pressure dependence study using chemical ionization mass spectrometry
Authors: Raventós‐Duran M. Teresa , McGillen Max , Percival Carl J. , Hamer Paul D. , Shallcross Dudley E. 
Published: July 2007

AbstractA temperature and pressure kinetic study for the CH3O2 + HO2 reaction has been performed using the turbulent flow technique with a chemical ionization mass spectrometry detection system. An Arrhenius expression was obtained for the overall rate coefficient of CH3O2 + HO2 reaction: k(T) = (3.82+2.79−1.61) × 10−13 exp[(−781 ± 127)/T] cm−3 molecule−1 s−1. A direct quantification of the branching ratios for the O3 and OH product channels, at pressures between 75 and 200 Torr and temperatures between 298 and 205 K, was also investigated. The atmospheric implications of considering the upper limit rate coefficients for the O3 and OH branching channels are observed with a significant reduction of the concentration of CH3OOH, which leads to a lower amount of methyl peroxy radical. © 2007 Wiley Periodicals, Inc. Int J Chem Kinet 39: 571–579, 2007
The unusual ability of α‐angelicalactone to form adducts: A kinetic approach
Authors: Fernández‐Rodríguez Estrella , Manso José A. , Pérez‐Prior M. Teresa , García‐Santos M. del Pilar , Calle Emilio , Casado Julio 
Published: July 2007

AbstractSince α‐angelicalactone (AAL) substantially inhibits the formation of tumors, here its chemical reactivity was compared with that of carcinogenic lactones. Investigation of the electrophilic potential of AAL was carried out by studying the capacity of this lactone to form adducts with NBP, 4‐(p‐nitrobenzyl)pyridine, a substrate with nucleophilic characteristics similar to DNA bases. The formation of the AAL–NBP adduct occurs about 900,000‐fold faster than with β‐propiolactone, the most effective carcinogenic lactone (ΔG#35 = 52 and 87 kJ mol−1, respectively). A stopped‐flow technique was required for this reaction to be monitored. It was concluded that the formation of AAL–NBP adducts takes place through an entropy‐strain‐catalyzed mechanism caused by early lactone ring cleavage. The kinetic results are consistent with the AAL potential as a chemoprotective agent. © 2007 Wiley Periodicals, Inc. Int J Chem Kinet 39: 591–594, 2007
Navier–Stokes equations with slip boundary conditions
Authors: Guo Ben‐yu 
Published: July 2007

AbstractIn this paper, we consider incompressible viscous fluid flows with slip boundary conditions. We first prove the existence of solutions of the unsteady Navier–Stokes equations in n‐spacial dimensions. Then, we investigate the stability, uniqueness and regularity of solutions in two and three spacial dimensions. In the compactness argument, we construct a special basis fulfilling the incompressibility exactly, which leads to an efficient and convergent spectral method. In particular, we avoid the main difficulty for ensuring the incompressibility of numerical solutions, which occurs in other numerical algorithms. We also derive the vorticity‐stream function form with exact boundary conditions, and establish some results on the existence, stability and uniqueness of its solutions. Copyright © 2007 John Wiley & Sons, Ltd.
Algebraic properties of evolution partial differential equations modelling prices of commodities
Authors: Sophocleous C. , Leach P. G. L. , Andriopoulos K. 
Published: July 2007

AbstractSchwartz (J. Finance 1997; 52:923–973) presented three models for the pricing of a commodity. The simplest was a variation on the Black–Scholes equation. The second allowed for a stochastic convenience yield on the commodity and the third added a stochastic variation in the underlying interest rate. We apply the techniques of Lie group analysis to resolve these equations, discuss their peculiar algebraic properties and indicate the route to the addition of other stochastic influences. Copyright © 2007 John Wiley & Sons, Ltd.
Long time behavior of a singular perturbation of the viscous Cahn–Hilliard–Gurtin equation
Authors: Bonfoh Ahmed , Grasselli Maurizio , Miranville Alain 
Published: July 2007

AbstractWe consider a singular perturbation of the generalized viscous Cahn–Hilliard equation based on constitutive equations introduced by Gurtin. This equation rules the order parameter ρ, which represents the density of atoms, and it is given on an‐rectangle (n⩽3) with periodic boundary conditions. We prove the existence of a family of exponential attractors that is robust with respect to the perturbation parameter ε>0, as ε goes to 0. In a similar spirit, we analyze the stability of the global attractor. Ifn=1, 2, then we also construct a family of inertial manifolds that is continuous with respect to ε. These results improve and generalize the ones contained in some previous papers. Finally, we establish the convergence of any trajectory to a single equilibriumviaa suitable version of the Łojasiewicz–Simon inequality, provided that the potential is real analytic. Copyright © 2007 John Wiley & Sons, Ltd.
A note on the universal stability of convective flow with variable viscosity
Authors: Saravanan S. , Brindha D. 
Published: July 2007

AbstractThe stability of convective motion of a fluid driven by volumetric heat sources and applied pressure gradient is investigated. The viscosity of the fluid is assumed to depend linearly on temperature. The sufficient conditions for the existence of such a flow are obtained based on the energy inequalities. Copyright © 2007 John Wiley & Sons, Ltd.