bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential oil supplementation in milk replacers: short- and long-term impacts on feed efficiency, the faecal microbiota and the plasma metabolome in dairy calves
Authors: Andrés Sonia , Gini Chiara , Ceciliani Fabrizio , Gutiérrez-Expósito Daniel , Arteche-Villasol Noive , Martín Alba , Cremonesi Paola , Faré Fiorenza , Ghaffari Morteza Hosseini , Giráldez F. Javier , Abdennebi-Najar Latifa 
Published: April 2024

Abstract
Early supplementation with oregano essential oil (EO) in milk replacer (MR) may improve growth, immune responses, the microbiota and the metabolome in dairy calves during pre-weaning and in adulthood. Sixteen female dairy calves (3 days of age) were divided in two groups (n = 8/group): the control group (no EO) and the EO group (0.23 ml of EO in MR during 45 days). After weaning, calves were kept in a feedlot and fed ad libitum. The animals were weighed, and blood and faecal samples were collected on days 3 (T0), 45 (T1) and 370 (T2) to measure the biochemical profile and characterise peripheral blood mononuclear cells (PBMCs; CD4+, CD8+, CD14+, CD21+ and WC1+), the metabolome and microbiota composition. The EO group only had greater average daily weight gain during the suckling (EO supplementation) period (P = 0.030). The EO group showed higher average CD14+ population (monocytes) values, a lower abundance of Ruminococcaceae UCG-014, Faecalibacterium, Blautia and Alloprevotella and increased abundances of Allistipes and Akkermansia. The modification of some metabolites in plasma, such as butyric acid, 3-indole-propionic acid and succinic acid, particularly at T1, are consistent with intestinal microbiota changes. The data suggest that early EO supplementation increases feed efficiency only during the suckling period with notable changes in the microbiota and plasma metabolome; however, not all of these changes can be considered desirable from a gut health point of view. Additional research studies is required to demonstrate that EOs are a viable natural alternative to antibiotics for improving calf growth performance and health.
Chemistry Contribution to Stratospheric Ozone Depletion After the Unprecedented Water‐Rich Hunga Tonga Eruption
Authors: Zhang Jun , Kinnison Douglas , Zhu Yunqian , Wang Xinyue , Tilmes Simone , Dube Kimberlee , Randel William 
Published: April 2024

AbstractFollowing the Hunga Tonga–Hunga Ha'apai (HTHH) eruption in January 2022, stratospheric ozone depletion was observed at Southern Hemisphere mid‐latitudes and over Antarctica during the 2022 austral wintertime and springtime, respectively. The eruption injected sulfur dioxide and unprecedented amounts of water vapor into the stratosphere. This work examines the chemistry contribution of the volcanic materials to ozone depletion using chemistry‐climate model simulations with nudged meteorology. Simulated 2022 ozone and nitrogen oxide (NOx = NO + NO2) anomalies show good agreement with satellite observations. We find that chemistry yields up to 4% ozone destruction at mid‐latitudes near ∼70 hPa in August and up to 20% ozone destruction over Antarctica near ∼80 hPa in October. Most of the ozone depletion is attributed to internal variability and dynamical changes forced by the eruption. Both the modeling and observations show a significant NOx reduction associated with the HTHH aerosol plume, indicating enhanced dinitrogen pentoxide hydrolysis on sulfate aerosol.
Observed Spatiotemporal Variability in the Annual Sea Level Cycle Along the Global Coast
Authors: Barroso A. , Wahl T. , Li S. , Enriquez A. , Morim J. , Dangendorf S. , Piecuch C. , Thompson P. 
Published: April 2024

AbstractChanges in the seasonal sea level cycle can modulate the flooding risk along coastlines. Here, we use harmonic analysis to quantify changes in the amplitude and phase of the annual component of the sea level cycle at 798 tide gauge locations along the global coastline where long records are available. We identify coastal hotspots by applying clustering methods revealing coherent regions with similar patterns of variability in the annual sea level cycle. Results show that for most tide gauges the annual amplitude reached its maximum after 1970 and its peak typically occurs during the fall season of the respective hemisphere. Many tide gauges exhibit non‐stationarity in the annual cycle in terms of amplitude and/or phase. For example, at 226 tide gauges we find significant trends in the amplitude (either increasing or decreasing) for the time period after 1970; while several sites (50 in total), mostly in the Mediterranean and around Pacific islands, experienced phase changes leading to shifts in the timing of the peak of the annual cycle by more than a month over their entire record. Our results highlight the importance of accounting for potential non‐stationarity in seasonal mean sea level cycles along coastlines.
Analyzing the Instabilities in the Venus Atmosphere Using Bred Vectors
Authors: Liang Jianyu , Sugimoto Norihiko , Miyoshi Takemasa 
Published: April 2024

AbstractBarotropic, baroclinic, and Rossby‐Kelvin instabilities exist in the Venus atmosphere, as revealed by previous studies using numerical models. This study aims to deepen our understanding of the instabilities in the Venus atmosphere using the breeding of growing modes (BGM) method for the first time. We conducted a 1‐year model simulation as the control run. First, we conducted identical twin experiments in which random perturbations were added to the control run at the initial time, and they grew freely. Perturbations in the upper layer (UL, 70–100 km altitudes) grow faster at the beginning but saturate earlier at a low value compared to those in the lower layer (LL, 40–70 km altitudes). Next, we implemented the BGM method and conducted multiple breeding cycle experiments with different rescaling amplitudes and intervals, the two parameters of the BGM method. The bred vectors (BVs) from these experiments identified instabilities in various regions. With large rescaling amplitudes, the structure and amplitude of the BVs in the LL closely resembled the deviations in the control run, indicating the growth of BVs due to barotropic, baroclinic, and Rossby‐Kelvin instabilities. Composite mean analysis shows larger BV amplitudes in the morning hemisphere at the 60–70 km altitudes in the mid‐latitudes, indicating enhanced baroclinic instability by the thermal tide. Finally, we estimated the intrinsic predictability related to baroclinic instability for Venus is >1 month, which would be longer than that for the Earth of ∼2 weeks.
Effects of Seasonal Variations in Seagrass Density and Storms on Sediment Retention and Connectivity Between Subtidal Flats and Intertidal Marsh
Authors: Zhu Qingguang , Wiberg Patricia L. 
Published: April 2024

AbstractBoth submerged aquatic vegetation and salt marsh are important coastal ecosystems known for their effectiveness in sediment trapping and carbon burial. However, their proximity can lead to competition for limited sediment resources, potentially compromising their capacity to facilitate sufficient sediment deposition to withstand sea‐level rise. Here we applied the Delft3D flow and sediment transport model in a shallow coastal bay to assess how seasonal variations in seagrass density and storms modulate sediment retention and connectivity between subtidal flats and intertidal marsh. Results show that submerged seagrass meadows acted as temporary storage for fine sediment and altered the timing of sediment transport to an adjacent marsh through seasonal growth cycles. When seagrass occupied subtidal flats, sediment flux to the marsh was controlled by seasonal seagrass density variations, with peak fluxes during winter senescence. In contrast, wave‐induced sediment resuspension on the flats was the major contributor for marsh sediment input in the simulation with no seagrass, with peak storm‐driven fluxes in summer/autumn. Overall, seagrass meadows significantly increased annual sediment accumulation on the vegetated subtidal flats by tenfold, while reducing annual marsh sediment flux and edge erosion by 20%. Seagrass meadows and marsh were both able to maintain a sediment deposition rate comparable to the rapid sea‐level rise at the study site despite the absence of terrestrial sediment sources. Our findings highlight the strong seasonal control subtidal aquatic vegetation has in the vertical and horizontal dynamics of tidal flat‐marsh systems and provide insights that can inform wetland restoration and management strategies in similar systems.
Entosis: the core mechanism and crosstalk with other cell death programs
Authors: Kim Sunghoon , Lee Donghyuk , Kim Sung Eun , Overholtzer Michael 
Published: April 2024

AbstractCell death pathways play critical roles in organism development and homeostasis as well as in the pathogenesis of various diseases. While studies over the last decade have elucidated numerous different forms of cell death that can eliminate cells in various contexts, how certain mechanisms impact physiology is still not well understood. Moreover, recent studies have shown that multiple forms cell death can occur in a cell population, with different forms of death eliminating individual cells. Here, we aim to describe the known molecular mechanisms of entosis, a non-apoptotic cell engulfment process, and discuss signaling mechanisms that control its induction as well as its possible crosstalk with other cell death mechanisms.
Mass-produced and uniformly luminescent photochromic fibers toward future interactive wearable displays
Authors: Li Yan , Su Yikai 
Published: April 2024

AbstractEnabling flexible fibers with light-emitting capabilities has the potential to revolutionize the design of smart wearable interactive devices. A recent publication in Light Science & Application, an interdisciplinary team of scientists led by Prof. Yan-Qing Lu and Prof. Guangming Tao has realized a highly flexible, uniformly luminescent photochromic fiber based on a mass-produced thermal drawing method. It overcomes the shortcomings of existing commercial light-diffusing fibers, exhibiting outstanding one-dimensional linear illumination performance. The research team integrated controllable photochromic fibers into various wearable interaction interfaces, providing a novel approach and insights to enable human-computer interaction.
Knowledge and attitudes toward anaphylaxis to local anesthetics in dental practice
Authors: Cherrez-Ojeda Ivan , Gallardo-Bastidas Juan C. , Borrero Gabriela Rouillon , Mautong Hans , Silva Paola Andrea Mena , Sarfraz Zouina , Sarfraz Azza , Cano Leonardo , Robles-Velasco Karla 
Published: April 2024

Abstract
Objective/aim
The absence of a comprehensive understanding of potential anaphylactic reactions to local anesthetics (LAs) and management can result in grave consequences. For this reason we aim to assess Latin American dentists’ knowledge, preparedness, and competency in managing anaphylactic reactions to LAs.

Materials and Methods
Design: A cross-sectional study was conducted from November 2021 to February 2022. Board-certified dentists answered a survey comprising 26 structured questions. Chi-square tests and logistic regression models were performed in Stata 17.0. Setting: Argentina, Brazil, Colombia, Costa Rica, Ecuador, Honduras, Mexico, Peru, Venezuela, and other Latin American countries.

Results
Of 507 respondents, lidocaine was the most frequently used LA (88.1%). While 85.2% could identify dyspnea as a symptom of anaphylaxis, only 50.1% knew the correct route for epinephrine administration, and just 43.5% had epinephrine in their emergency kits. Confidence in managing anaphylactic reactions was low (9.6%). Older age was inversely related to both knowledge of anaphylaxis management and the possession of epinephrine (P = 0.003 and P = 0.0001, respectively).

Discussion
Our study highlights a concerning discrepancy between the practical readiness of Latin American dentists in handling anaphylaxis.

Conclusion
The study’s findings underscore the need for educational interventions to improve the readiness to identify and handle anaphylactic emergencies in dental practice.
Cellular metabolism regulates the differentiation and function of T-cell subsets
Authors: Ma Sicong , Ming Yanan , Wu Jingxia , Cui Guoliang 
Published: April 2024

AbstractT cells are an important component of adaptive immunity and protect the host from infectious diseases and cancers. However, uncontrolled T cell immunity may cause autoimmune disorders. In both situations, antigen-specific T cells undergo clonal expansion upon the engagement and activation of antigens. Cellular metabolism is reprogrammed to meet the increase in bioenergetic and biosynthetic demands associated with effector T cell expansion. Metabolites not only serve as building blocks or energy sources to fuel cell growth and expansion but also regulate a broad spectrum of cellular signals that instruct the differentiation of multiple T cell subsets. The realm of immunometabolism research is undergoing swift advancements. Encapsulating all the recent progress within this concise review in not possible. Instead, our objective is to provide a succinct introduction to this swiftly progressing research, concentrating on the metabolic intricacies of three pivotal nutrient classes—lipids, glucose, and amino acids—in T cells. We shed light on recent investigations elucidating the roles of these three groups of metabolites in mediating the metabolic and immune functions of T cells. Moreover, we delve into the prospect of “editing” metabolic pathways within T cells using pharmacological or genetic approaches, with the aim of synergizing this approach with existing immunotherapies and enhancing the efficacy of antitumor and antiinfection immune responses.
De novo variants in GABRA4 are associated with a neurological phenotype including developmental delay, behavioral abnormalities and epilepsy
Authors: Sajan Samin A. , Gradisch Ralph , Vogel Florian D. , Coffey Alison J. , Salyakina Daria , Soler Diana , Jayakar Parul , Jayakar Anuj , Bianconi Simona E. , Cooper Annina H. , Liu Shuxi , William Nancy , Benkel-Herrenbrück Ira , Maiwald Robert , Heller Corina , Biskup Saskia , Leiz Steffen , Westphal Dominik S. , Wagner Matias , Clarke Amy , Stockner Thomas , Ernst Margot , Kesari Akanchha , Krenn Martin 
Published: April 2024

AbstractNine out of 19 genes encoding GABAA receptor subunits have been linked to monogenic syndromes characterized by seizures and developmental disorders. Previously, we reported the de novo variant p.(Thr300Ile) in GABRA4 in a patient with epilepsy and neurodevelopmental abnormalities. However, no new cases have been reported since then. Through an international collaboration, we collected molecular and phenotype data of individuals carrying de novo variants in GABRA4. Patients and their parents were investigated either by exome or genome sequencing, followed by targeted Sanger sequencing in some cases. All variants within the transmembrane domain, including the previously reported p.(Thr300Ile) variant, were characterized in silico and analyzed by molecular dynamics (MD) simulation studies. We identified three novel de novo missense variants in GABRA4 (NM_000809.4): c.797 C > T, p.(Pro266Leu), c.899 C > A, p.(Thr300Asn), and c.634 G > A, p.(Val212Ile). The p.(Thr300Asn) variant impacts the same codon as the previously reported variant p.(Thr300Ile) and likely arose post-zygotically as evidenced by sequencing oral mucosal cells. Overlapping phenotypes among affected individuals included developmental delay (4/4), epileptiform EEG abnormalities (3/4), attention deficits (3/4), seizures (2/4), autistic features (2/4) and structural brain abnormalities (2/4). MD simulations of the three variants within the transmembrane domain of the receptor indicate that sub-microsecond scale dynamics differ between wild-type and mutated subunits. Taken together, our findings further corroborate an association between GABRA4 and a neurological phenotype including variable neurodevelopmental, behavioral and epileptic abnormalities.
Archaeological and molecular evidence for ancient chickens in Central Asia
Authors: Peters Carli , Richter Kristine K. , Wilkin Shevan , Stark Sören , Mir-Makhamad Basira , Fernandes Ricardo , Maksudov Farhod , Mirzaakhmedov Sirojidin , Rahmonov Husniddin , Schirmer Stefanie , Ashastina Kseniia , Begmatov Alisher , Frachetti Michael , Kurbanov Sharof , Shenkar Michael , Hermes Taylor , Kidd Fiona , Omelchenko Andrey , Huber Barbara , Boivin Nicole , Wang Shujing , Lurje Pavel , von Baeyer Madelynn , Dal Martello Rita , Spengler Robert N. 
Published: April 2024

AbstractThe origins and dispersal of the chicken across the ancient world remains one of the most enigmatic questions regarding Eurasian domesticated animals. The lack of agreement concerning timing and centers of origin is due to issues with morphological identifications, a lack of direct dating, and poor preservation of thin, brittle bird bones. Here we show that chickens were widely raised across southern Central Asia from the fourth century BC through medieval periods, likely dispersing along the ancient Silk Road. We present archaeological and molecular evidence for the raising of chickens for egg production, based on material from 12 different archaeological sites spanning a millennium and a half. These eggshells were recovered in high abundance at all of these sites, suggesting that chickens may have been an important part of the overall diet and that chickens may have lost seasonal egg-laying
Experimentally realized physical-model-based frugal wave control in metasurface-programmable complex media
Authors: Sol Jérôme , Prod’homme Hugo , Le Magoarou Luc , del Hougne Philipp 
Published: April 2024

AbstractMetasurface-programmable radio environments are considered a key ingredient of next-generation wireless networks. Yet, identifying a metasurface configuration that yields a desired wireless functionality in an unknown complex environment was so far only achieved with closed-loop iterative feedback schemes. Here, we introduce open-loop wave control in metasurface-programmable complex media by estimating the parameters of a compact physics-based forward model. Our experiments demonstrate orders-of-magnitude advantages over deep-learning-based digital-twin benchmarks in terms of accuracy, compactness and required calibration examples. Strikingly, our parameter estimation also works without phase information and without providing measurements for all considered scattering coefficients. These unique generalization capabilities of our pure-physics model unlock unforeseen and previously inaccessible frugal wave control protocols that significantly alleviate the measurement complexity. For instance, we achieve coherent wave control (focusing or perfect absorption) and phase-shift-keying backscatter communications in metasurface-programmable complex media with intensity-only measurements. Our approach is also directly relevant to dynamic metasurface antennas, microwave-based signal processors and emerging in situ reconfigurable nanophotonic, optical and room-acoustical systems.
Endocytic vesicles act as vehicles for glucose uptake in response to growth factor stimulation
Authors: Tsutsumi Ryouhei , Ueberheide Beatrix , Liang Feng-Xia , Neel Benjamin G. , Sakai Ryuichi , Saito Yoshiro 
Published: April 2024

AbstractGlycolysis is a fundamental cellular process, yet its regulatory mechanisms remain incompletely understood. Here, we show that a subset of glucose transporter 1 (GLUT1/SLC2A1) co-endocytoses with platelet-derived growth factor (PDGF) receptor (PDGFR) upon PDGF-stimulation. Furthermore, multiple glycolytic enzymes localize to these endocytosed PDGFR/GLUT1-containing vesicles adjacent to mitochondria. Contrary to current models, which emphasize the importance of glucose transporters on the cell surface, we find that PDGF-stimulated glucose uptake depends on receptor/transporter endocytosis. Our results suggest that growth factors generate glucose-loaded endocytic vesicles that deliver glucose to the glycolytic machinery in proximity to mitochondria, and argue for a new layer of regulation for glycolytic control governed by cellular membrane dynamics.
Janus electronic state of supported iridium nanoclusters for sustainable alkaline water electrolysis
Authors: Liu Yaoda , Li Lei , Wang Li , Li Na , Zhao Xiaoxu , Chen Ya , Sakthivel Thangavel , Dai Zhengfei 
Published: April 2024

AbstractMetal-support electronic interactions play crucial roles in triggering the hydrogen spillover (HSo) to boost hydrogen evolution reaction (HER). It requires the supported metal of electron-rich state to facilitate the proton adsorption/spillover. However, this electron-rich metal state contradicts the traditional metal→support electron transfer protocol and is not compatible with the electron-donating oxygen evolution reaction (OER), especially in proton-poor alkaline conditions. Here we profile an Ir/NiPS3 support structure to study the Ir electronic states and performances in HSo/OER-integrated alkaline water electrolysis. The supported Ir is evidenced with Janus electron-rich and electron-poor states at the tip and interface regions to respectively facilitate the HSo and OER processes. Resultantly, the water electrolysis (WE) is efficiently implemented with 1.51 V at 10 mA cm–2 for 1000 h in 1 M KOH and 1.44 V in urea-KOH electrolyte. This research clarifies the Janus electronic state as fundamental in rationalizing efficient metal-support WE catalysts.
Intron detention tightly regulates the stemness/differentiation switch in the adult neurogenic niche
Authors: González-Iglesias Ainara , Arcas Aida , Domingo-Muelas Ana , Mancini Estefania , Galcerán Joan , Valcárcel Juan , Fariñas Isabel , Nieto M. Angela 
Published: April 2024

AbstractThe adult mammalian brain retains some capacity to replenish neurons and glia, holding promise for brain regeneration. Thus, understanding the mechanisms controlling adult neural stem cell (NSC) differentiation is crucial. Paradoxically, adult NSCs in the subependymal zone transcribe genes associated with both multipotency maintenance and neural differentiation, but the mechanism that prevents conflicts in fate decisions due to these opposing transcriptional programmes is unknown. Here we describe intron detention as such control mechanism. In NSCs, while multiple mRNAs from stemness genes are spliced and exported to the cytoplasm, transcripts from differentiation genes remain unspliced and detained in the nucleus, and the opposite is true under neural differentiation conditions. We also show that m6A methylation is the mechanism that releases intron detention and triggers nuclear export, enabling rapid and synchronized responses. m6A RNA methylation operates as an on/off switch for transcripts with antagonistic functions, tightly controlling the timing of NSCs commitment to differentiation.