bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum concentrations of salicylate and naproxen during concurrent therapy in patients with rheumatoid arthritis
Authors: Furst Daniel E. , Sarkissian Edmund , Blocka Kenneth , Cassell Sidney , Dromgoole Sydney , Harris E. Robert , Hirschberg Joel M. , Josephson Nathan , Paulus Harold E. 
Published: February 2007

AbstractThe kinetic interaction between salicylate and naproxen was investigated in 25 rheumatoid arthritis patients. Kinetic interactions were tested in serum after patients had been on each drug regimen for 1 month. Salicylate decreased serum naproxen concentration from 89.5 mg/liter to 65.9 mg/liter (P < 0.001) and increased serum naproxen clearance by 56%. Naproxen had minimal effect on serum salicylate concentrations.
Charge‐charge interactions between articular cartilage and cationic antibodies, antigens, and immune complexes
Authors: Zatarain‐Rios Ernesto , Mannik Mart 
Published: February 2007

AbstractBovine articular cartilage was used to examine the binding of cationized antibodies, antigens, and immune complexes to articular cartilage by charge‐charge interactions. Rabbit antibodies to human serum albumin (anti‐HSA) and HSA were cationized to various degrees by the addition of amino groups. When approximately 20 or more new amino groups were added to HSA and approximately 25 or more amino groups were added to anti‐HSA, the proteins readily bound to cartilage and penetrated into the matrix. Soluble immune complexes made with the cationic antibodies, including small‐latticed complexes, bound only to the surface of the cartilage. When cationic HSA was bound to the matrix of the cartilage, unaltered antibodies bound only to the antigen at the surface and did not penetrate the matrix. This model system defines the manner in which cationic antigens and antibodies bind to and penetrate into the articular cartilage.
Quantitation of retroviral gp70 antigen, autoantibodies, and immune complexes in extravascular space in arthritic mrl‐lpr/lpr mice. use of a subcutaneously implanted tissue cage model
Authors: Dayer Eric , Yoshida Haruyoshi , Izui Shozo , Lambert Paul‐Henri 
Published: February 2007

AbstractMRL‐lpr/lpr (MRL/1) mice spontaneously develop a disease that is characterized by glomerulonephritis, diffuse vasculitis, and arthritis associated with high levels of autoantibodies that include IgG rheumatoid factor (RF). To define the immunopathogenic mechanisms that lead to the development of extravascular lesions such as arthritis, we implanted a tissue cage subcutaneously in arthritic MRL/1 mice and compared components of the tissue cage fluid, which resembles the extravascular fluid, with those of sera. When compared with those of sera, tissue cage fluids from arthritic MRL/1 mice had similar levels of RF and one‐third the amount of Clq immune complexes. In contrast, anti‐DNA activities in tissue cage fluids corresponded to only 10% of the serum activities and, most strikingly, nephritogenic retroviral gp70‐anti‐gp70 immune complexes were almost undetectable in tissue cage fluids. This was also the case for another strain of autoimmune mice, (New Zealand black x New Zealand white)F1 mice, although they did not produce RF. The absence of gp70 immune complexes in tissue cage fluids could be due to markedly limited diffusion of gp70 antigen in these fluids. These results strongly suggest that serum proteins, including autoantigen and autoantibodies, appear in extravascular fluid in a selective manner, depending on their size and charge. Their specific properties in sera or extravascular fluid could partly account for the different manifestations of vascular and extravascular lesions observed in autoimmune mice.
Measuring disease activity: comparison of joint tenderness, swelling, and ultrasonography in rheumatoid arthritis
Authors: Spiegel Timothy M. , Iii William King , Weiner Steven R. , Paulus Harold E. 
Published: February 2007

AbstractIn a prospective study of 6 patients with classic rheumatoid arthritis, we evaluated 3 measures of disease activity: comprehensive joint swelling and joint tenderness counts, and ultrasonography of joints. After baseline data were obtained on these 6 patients, therapy with fenbufen, a new nonsteroidal antiinflammatory drug, was begun. Followup examinations were performed at 4 weeks and 24 weeks after baseline. There were statistically significant differences between joint tenderness and joint swelling findings and between joint tenderness and joint ultrasonography findings (P < 0.05 by kappa test statistic). In comparisons of joint swelling and ultrasonography, no difference was found (P > 0.05). When measures of changes over 6 months were compared, there was a high concordance between improvement in joint swelling and improvement in joint ultrasonography (P < 0.01). Our results demonstrate that clinical assessment of joint swelling provides an accurate measure of synovial effusion, as confirmed by the more objective ultrasound measurements.
Connective tissue activation. xxxii. structural and biologic characteristics of mesenchymal cell—derived connective tissue activating peptide—v
Authors: Cabral A. R. , Cole L. A. , Walz D. A. , Castor C. W. 
Published: February 2007

AbstractConnective tissue activating peptide—V (CTAP‐V) is a single‐chain, mesenchymal cell—derived anionic protein with large and small molecular forms (Mr of 28,000 and 16,000, respectively), as defined by sodium dodecyl sulfate—polyacrylamide gel electrophoresis. The proteins have similar specific activities with respect to stimulation of hyaluronic acid and DNA formation in human synovial fibroblast cultures. S‐carboxymethylation or removal of sialic acid residues did not modify CTAP‐V biologic activity. Rabbit antibodies raised separately against each of the purified CTAP‐V proteins reacted, on immunodiffusion and on Western blot, with each antigen and neutralized mitogenic activity. The amino‐terminal amino acid sequence of the CTAP‐V proteins, determined by 2 laboratories, confirmed their structural similarities. The amino‐terminal sequence through 37 residues was demonstrated for the smaller protein. The first 10 residues of CTAP‐V (28 kd) were identical to the N‐terminal decapeptide of CTAP‐V (16 kd). The C‐terminal sequence, determined by carboxy‐peptidase Y digestion, was the same for both CTAP‐V molecular species. The 2 CTAP‐V peptides had similar amino acid compositions, whether residues were expressed as a percent of the total or were normalized to mannose. Reduction of native CTAP‐V protein released sulfhydryl groups in a protein:disulfide ratio of 1:2; this suggests that CTAP‐V contains 2 intramolecular disulfide bonds. Clearly, CTAP‐V is a glycoprotein. The carbohydrate content of CTAP‐V (16 kd) and CTAP‐V (28 kd) is 27% and 25%, respectively. CTAP‐V may have significance in relation to autocrine mechanisms for growth regulation of connective tissue cells and other cell types.
Acute arthritis occurring in association with subcutaneous dirofilaria tenuis infection
Authors: Corman Lourdes C. 
Published: February 2007

AbstractA patient who developed a self‐limited acute migratory arthritis in association with subcutaneous infection with Dirofilaria tenuis is described. There have been several reports of parasitic rheumatism and the possible association between arthritis and parasitic infection. Parasitic rheumatism in a patient infected with D tenuis has not been previously reported, however.
Increased helper inducer and decreased suppressor inducer phenotypes in the rheumatoid joint
Authors: Lasky H. Paul , Bauer Kenneth , Pope Richard M. 
Published: February 2007

AbstractCells isolated from the joints of patients with rheumatoid arthritis (RA) exhibit functional immune abnormalities, such as diminished suppressor activity, depressed response to mitogens, and enhanced immunoglobulin production. We sought to characterize the T lymphocyte subsets in the synovial fluid (SF) and peripheral blood (PB) of RA patients in an attempt to clarify the mechanism(s) responsible for these functional immune abnormalities. We used dual‐immunofluorescence staining techniques with several combinations of monoclonal antibodies, including anti‐4B4 and anti‐2H4, which define, respectively, the helper inducer and suppressor inducer subsets of CD4+ (Leu‐3+ and T4+) cells. Mononuclear cells from normal PB (n = 9), RA PB (n = 6), and RA SF (n = 9) were analyzed, after staining, by flow cytometry. We observed a significant increase (P < 0.0002) in the number of cells bearing the helper inducer phenotype (CD4+, 4B4+), and a significant decrease (P < 0.0002) in the number of cells bearing the suppressor inducer phenotype (CD4+, 2H4+), in RA SF compared with the levels in PB from RA patients or normal control subjects. We also observed that the CD8+, 2H4+ subset was significantly decreased (P < 0.0001) in SF compared with that in PB. There was no significant difference in the lymphocyte subset levels in PB from RA patients and from normal subjects. These observations may account, in part, for the reduced suppressor activity, the poor response to mitogens, and the autologous mixed lymphocyte reaction, as well as the enhanced production of Ig and rheumatoid factor, that are observed in the rheumatoid joint.
Monoclonal rheumatoid factor–igg immune complexes. poor fixation of opsonic c4 and c3 despite efficient complement activation
Authors: Ng Yin C. , Peters D. Keith , Walport Mark J. 
Published: February 2007

AbstractMonoclonal IgM rheumatoid factor forms complexes with IgG in essential mixed cryoglobulinemia. We demonstrate that such complexes fix C3 and C4 poorly, although efficient fluid‐phase C3 conversion can occur. Fixation of small amounts of C4 may be sufficient to generate a C3 convertase, but may prevent subsequent fixation of C3 by competing for binding sites on the complex. These complexes bind inefficiently to normal erythrocyte complement receptor type 1 (CR1) in vitro, and are undetectable on erythrocytes of patients with essential mixed cryoglobulinemia in vivo. Clearance of such phlogistic complexes from tissues by CR1‐bearing cells may be inefficient.
A new autoantibody in patients with rheumatoid arthritis: characterization of the anti–hat‐1 antibody system
Authors: Abe Yoichi , Inada Shinichi , Torikai Katsutaka 
Published: February 2007

AbstractA unique, new autoantibody, anti–HaT‐1, has been identified in the serum of patients with rheumatoid arthritis (RA). Anti–HaT‐1 antibody is predominantly IgM, and reacts by double immunodiffusion with HaT‐1 antigen, an acidic protein in human and rat liver supernatant (MW ∼ 150,000). It was detected in 9 of 38 patients with RA, 2 of 15 patients with RA and Sjögren's syndrome, and none of 92 patients with other connective tissue diseases, indicating that this autoantibody is highly specific for RA.
Radiographic assessment and psychologic variables as predictors of pain and functional impairment in osteoarthritis of the knee or hip
Authors: Summers Mary N. , Haley William E. , Reveille John D. , AlarcOan Graciela S. 
Published: February 2007

AbstractSixty‐five outpatients with osteoarthritis of the knee and/or hip were assessed using radiographic ratings of disease severity, measures of psychologic coping, and multidimensional clinical outcomes of degree of pain and functional impairment. Disease severity accounted for little of the individual variability in clinical outcomes. Even after controlling for disease severity, psychologic variables remained strong predictors of individual differences in functional impairment and pain. Psychologic processes deserve greater clinical and research attention as potential mediators between disease severity and clinical outcome.
Articular responses to purified cartilage proteoglycans
Authors: Boniface Raymond J. , Cain Paul R. , Evans Christopher H. 
Published: February 2007

AbstractTo examine whether proteoglycans (PGs) liberated from cartilage might contribute to articular changes in arthritis, cartilage PGs were injected intraarticularly into rabbit knee joints. Twice‐weekly injections of PG (2.5 mg) provoked synovial hypertrophy, synovitis, erosion of the articulating surfaces, and loss of metachromasia of the articular cartilage. These changes were accompanied by a marked elevation in the production of neutral collagenase and gelatinase by both synoviocytes and chondrocytes. The synoviocytes of experimental knee joints also produced factor(s), possibly related to interleukin‐1, which provoked the activation of chondrocytes. Our data are consistent with the idea that free PG fragments mediate some of the pathophysiologic changes that occur in arthritic joints. This property may be particularly important in osteoarthritis.
Acquired immunodeficiency syndrome mimicking sjögren's syndrome and systemic lupus erythematosus
Authors: De Clerck Luc S. , Couttenye Marie M. , De Broe Marc E. , Stevens Wim J. 
Published: February 2007

AbstractWe describe a patient in whom the diagnosis of transfusion‐associated acquired immunodeficiency syndrome (AIDS) was delayed because her clinical symptoms were similar to those of systemic lupus erythematosus and Sjögren's syndrome and because of a false‐negative result on a Western blot test for human immunodeficiency virus. The importance of using different diagnostic tests for AIDS and the pitfalls in diagnosing AIDS in patients who appear to have connective tissue disease are discussed.
Degenerative spondylolisthesis, synovial cyst of the zygapophyseal joints, and sciatic syndrome: report of two cases and review of the literature
Authors: Reust Pierre , Wendling Daniel , Lagier RenEa , Pageaut GEarard , Reverdin Alain , Jacquet Guy , Guidet Michel , Fallet Georges H. 
Published: February 2007

AbstractTwo cases of sciatica secondary to nerve root compression by a “synovial cyst” of a zygapophyseal joint are described. In light of these 2 cases and on reviewing the literature, it appears that zygapophyseal joint osteoarthritis with degenerative (or articular) spondylolisthesis can be a predisposing factor to the formation of such synovial expansions and, consequently, the cause of nerve root compression. The coexistence of a lumbar degenerative spondylolisthesis with a radicular syndrome should therefore encourage early investigation by computed tomography scan, so that conservative treatment would not be unnecessarily prolonged.
Clinical associations of anticentromere antibodies and antibodies to topoisomerase i. a study of 355 patients
Authors: Weiner E. S. , Earnshaw W. C. , SeneAcal J.‐L. , Bordwell B. , Johnson P. , Rothfield N. F. 
Published: February 2007

AbstractAnticentromere antibodies (ACA) and anti–topoisomerase I (anti–topo I) were assayed in serum samples from 355 patients: 89 with proximal scleroderma; 54 with CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias), without proximal scleroderma; 154 with primary and secondary Raynaud's disease; and 58 with other rheumatic diseases, without Raynaud's disease. Sera from healthy control subjects were also assayed. Using immunoblotting techniques, anti‐topo I was detected in 28% of the patients with proximal scleroderma; using immunodiffusion techniques, this antibody was found in only 20% of the same group of patients. Anti–topo I and ACA were found primarily in patients with scleroderma, CREST syndrome, and Raynaud's phenomenon. ACA identified patients with less severe disease, whereas anti–topo I identified patients with skin and cardiac involvement and patients with malignancies.
Epidemiology of reiter's syndrome in rochester, minnesota: 1950–1980
Authors: Michet Clement J. , Machado E. B. V. , Ballard David J. , Mckenna Charles H. 
Published: February 2007

AbstractThe incidence of Reiter's syndrome in a predominantly white, community‐based population is reported. The age‐adjusted annual incidence rate for males younger than age 50 was 3.5 per 100,000. No female cases were identified. Over time, a greater proportion of cases have been identified among younger males. In 63% of the patients, either a prolonged or relapsing disease course occurred.
Common tests for rheumatoid factors: poorly standardized but ubiquitous
Authors: Goddard David H. , Moore Mary E. 
Published: February 2007

AbstractRheumatoid factor (RF) test results reported in the College of American Pathologists' surveys for 1983–1985 lacked inter‐laboratory reliability and mutual validity. Using the 4 most popular commercial kits for RF testing, participating laboratories consistently identified as “positive” or “negative” all but the weakly positive samples. A wide range of titers was reported on qualitative testing, however. One popular kit using a modified sheep red blood cell agglutination technique yielded results that differed markedly from those with other kits. Investigators apparently have paid little attention to these discrepancies. In Arthritis & Rheumatism, from 1983 to 1985, over 50% of the articles that referred directly or indirectly to RFs omitted details of RF methodology. Until a reliable RF test is adopted, it is essential that such methodologic information be specified.
Correlates between autoantibodies to nucleolar antigens and clinical features in patients with systemic sclerosis (scleroderma)
Authors: Reimer Georg , Steen Virginia D. , Penning Carol A. , Medsger Thomas A. , Tan Eng M. 
Published: February 2007

AbstractImmunofluorescence on rat liver sections was used to select high‐titer antinucleolar antibodies (ANoA) in the sera of patients with systemic sclerosis (scleroderma). In 646 patients, 53 ANoA sera (8%) were identified, and of these, 46 were available in sufficient quantities for further analysis. The complex of RNA polymerase I was immunoprecipitated by 7 sera (15%), which uniformly produced punctate nucleolar staining. The PM‐Scl antigen, a particle consisting of 11 polypep‐tides, was immunoprecipitated by 8 sera (17%), all of which displayed homogeneous nucleolar staining. A 34‐kd nucleolar protein (fibrillarin) of the U3 RNP complex was positive in immunoblotting of 22 sera (48%), which characteristically produced clumpy nucleolar staining. Antibodies against RNA polymerase I were associated with diffuse scleroderma of short duration, which was characterized by a high prevalence of internal organ involvement, including renal crisis. Anti‐U3 RNP antibodies had a high prevalence in men with significantly less joint involvement, compared with ANoA‐negative patients. Anti–PM‐Scl antibodies identified a group of scleroderma patients with a high prevalence of concomitant myositis and renal involvement.
Spontaneous cartilage degeneration in guinea pigs
Authors: Bendele A. M. , Hulman J. F. 
Published: February 2007

AbstractSpontaneous degeneration of the knee joint cartilage in male Hartley guinea pigs was studied by light microscopy in animals aged 3–18 months. Unilateral focal degeneration, characterized by chondrocyte death and proteoglycan loss with surface fibrillation, was observed on the medial tibial plateau in 2 of 5 guinea pigs that were 3 months old. The incidence and severity of the lesions increased with age, and by 12–18 months of age, all animals had moderate‐to‐severe degeneration of the medial tibial plateau, femoral condyle, and meniscus. Lesions were not present on the lateral aspect of the knee joint in any of the animals.
Hairy cell leukemia presenting as a lupus‐like illness
Authors: Strickland Roger W. , Limmani Alex , Wall James G. , Krishnan Jayashree 
Published: February 2007

AbstractWe describe a young Hispanic man who presented with features suggestive of a diagnosis of systemic lupus erythematosus. Eventually, a bone marrow biopsy was performed, and it revealed the presence of hairy cell leukemia. This case of a lupus‐like syndrome should be added to the list of reported rheumatic syndromes that are associated with hairy cell leukemia.
A recombinant autoantigen derived from the human (u1) small nuclear rnp‐specific 68‐kd protein
Authors: Netter Hans Jürgen , Guldner Hans Herbert , Szostecki Carin , Lakomek Heinz‐Jürgen , Will Hans 
Published: February 2007

AbstractA human liver complementary DNA expression library was screened using sera from patients with high titers of autoantibodies, to search for clones expressing major autoantigens that are relevant in connective tissue diseases. One of the clones isolated expressed a major epitope(s) that was immunoreactive with anti‐U1 RNP sera, as shown by several techniques. Affinity‐purified autoantibodies from the cloned RNP protein specifically recognized the 68‐kd U1 RNP protein of HeLa cell nuclear extracts. All sera containing anti‐U1 RNP antibodies detected by immunodiffusion, counterimmuno‐electrophoresis, or immunoblotting also recognized the cloned RNP protein. The RNP antigen‐expressing bacterial colonies and the partially purified cloned RNP fusion protein have been applied to fast and sensitive immunologic assays for the detection and quantification of anti‐U1 RNP antibodies.
A controlled trial comparing sulfasalazine, gold sodium thiomalate, and placebo in rheumatoid arthritis
Authors: Williams H. J. , Ward J. R. , Dahl S. L. , Clegg D. O. , Willkens R. F. , Oglesby T. , Weisman M. H. , Schlegel S. , Michaels R. M. , Luggen M. E. , Polisson R. P. , Singer J. Z. , Kantor S. M. , Shiroky J. B. , Small R. E. , Gomez M. I. , Reading J. C. , Egger M. J. 
Published: February 2007

AbstractOne hundred eighty‐six patients with active rheumatoid arthritis were evaluated in a double‐blind, randomized study that compared treatment with sulfasalazine (SSZ) (2 gm/day), gold sodium thiomalate (GST) (50 mg/week), and placebo (PBO). The 37—week course of therapy was completed by 109 patients. While marked improvement was seen in all 3 treatment groups, the only statistically significant differences between SSZ or GST and PBO were in a decreased erythrocyte sedimentation rate and increased grip strength in the right hand. GST is known to be superior to PBO, and the response of the GST‐treated group was similar to that seen in other trials. The response of the PBO group, however, was much greater than in other placebo groups we have studied. SSZ was similar in efficacy to injectable gold, but was better tolerated. Because of adverse drug reactions (most commonly, rash, stomatitis, and proteinuria), 41% of patients were withdrawn from the GST treatment. Untoward drug effects (most frequently, rash and gastrointestinal distress) caused 16% of patients to be withdrawn from SSZ therapy.
Chondrocalcinosis in surgically resected joints
Authors: Sokoloff Leon , Varma Andre A. 
Published: February 2007

AbstractTo investigate the association between chondrocalcinosis (CC) and osteoarthritis (OA), 338 joint specimens were examined histologically (55 knees and 84 hips surgically resected because of idiopathic OA, 106 control knees obtained postmortem, and 93 fractured hips). The risk for CC in the OA knees was sixfold that of the age‐and sex‐adjusted control sections. CC occurred much less frequently in the hip than in the knee; the association with OA was less clear‐cut. It was not possible to resolve by statistical analysis which of the two pathological processes was the horse and which was the cart.
Antibodies against native type ii collagen do not precede the clinical onset of rheumatoid arthritis
Authors: Möttönen Timo , Hannonen Pekka , Oka Martti , Rautiainen Jaakko , Jokinen Ilmari , Arvilommi Heikki , Palosuo Timo , Aho Kimmo 
Published: February 2007

AbstractSerum levels of IgG and IgM antibodies to native human type II collagen were determined in 22 pre‐illness sera from subjects who developed seropositive rheumatoid arthritis (RA) 4 months to 5 years after sera were obtained, in 51 specimens from 35 healthy controls, and in 4–5 specimens from 58 patients with recent‐onset RA. The antibody levels in all pre‐illness serum specimens fell within the range seen for the healthy controls. Four RA patients had an IgG class antibody level and 4 had an IgM class antibody level that was above the highest level observed for controls, in at least 1 serum sample. No significant difference in the mean level of anticollagen antibodies was observed in the followup specimens from RA patients.