bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decreased natural killer activity in patients with liver cirrhosis
Authors: Nakamura Tatsuya , Morizane Toshio , Watanabe Tetsu , Tsuchimoto Kanji , Inagaki Yasutaka , Kumagai Naoki , Tsuchiya Masaharu 
Published: February 2007

AbstractNatural killer (NK) activity of peripheral blood mono‐nuclear cells from patients with non‐alcoholic liver cirrhosis (LC) (30 cases), patients with other, non‐malignant diseases (41 cases), and healthy subjects (36 cases) were investigated using 51Cr‐labelled CCRF‐CEM and K562 target cells. NK activity in patients with LC was lower than that in healthy subjects and that in patients with other, non‐malignant diseases. The differences were statistically significant by Student's t‐test and the Wilcoxon rank test. There was no difference of NK activity between serologically hepatitis B surface antigen (HBsAg)‐positive and ‐negative patients.
Antibodies against three purified structural proteins of the human type‐C retrovirus, HTLV, in Japanese adult T‐cell leukemia patients, healthy family members, and unrelated normals
Authors: Schüpbach Jörg , Kalyanaraman V. S. , Sarngadharan M. G. , Nakao Y. , Ito Y. , Gallo Robert C. 
Published: February 2007

AbstractThe immunological relationship of human T‐cell leukemia/lymphoma virus (HTLV) and the virus found in Japanese adult T‐cell leukemia/lymphoma (ATL) was investigated in detail by testing the specific binding of serum antibodies from Japanese ATL patients and normal Japanese donors to the purified HTLV proteins p24, p19, and p15. Sera were prescreened for antibodies to p24. Of those positive, 67% of the ATL sera and 78% of the normal sera were further shown to have antibodies against p19. In both groups 17% had antibodies to p15. Generally, the average antibody titers were twice as high in ATL as in normal sera. Competition radioimmunoprecipitation assays done with various sera and involving HTLV‐producing cells, virus‐positive cells from a Japanese ATL patient, and virus‐positive cultured T cells of one of his healthy family members as competing materials demonstrated no differences between the p24, p19, and p15 found in these cells. These results provide strong and detailed immunological evidence that the human retrovirus isolates first made from US patients with cutaneous T‐cell malignancies, and those made later in Japanese ATL are either identical or very closely related strains of the same virus, HTLV, a finding verified in other detailed analyses of the HTLV genomes of the respective isolates. To date only HTLV‐II, isolated from a US case of hairy‐cell leukemia of a T‐cell type, is a distinct additional human retrovirus class.
Inhibition of initiator‐induced SV40 gene amplification in SV40‐transformed Chinese hamster cells by infection with a defective parvovirus
Authors: Schlehofer Jörg R. , Heilbronn Regine , Georg‐Fries Brigitte , Hausen Harald Zur 
Published: February 2007

AbstractAmplification of SV40 genes in SV40‐transformed Chinese hamster embryo cells (CO631) by chemical carcinogens as well as by herpes simplex virus infection can be inhibited by infection with the defective parvovirus, AAV‐5. This is shown by in situ hybridization with SV40 DNA of AAV‐5‐infected CO631 cells after treatment with herpes simplex virus type I or with chemical carcinogens: the initiator‐induced selective amplification of SV40 sequences is prevented in the presence of the parvovirus. During HSV‐infection and also in the presence of carcinogens, in CO631 cells parvovirus DNA is synthesized and AAV‐5‐specific antigens are expressed as revealed by hybridization with cloned AAV‐5 DNA or by immuno‐fluorescence with monoclonal antibodies, respectively. The inhibition of initiator‐induced gene amplification could point to the mechanism of parvovirus‐mediated inhibition of tumor development and may indicate an important role of selective gene amplification in oncogenesis.
Immunoaffinity chromatography of a cellular tumor antigen from mouse neuroblastoma cells
Authors: Levitsky Kenneth , Chandrasekaran Krish , Mora Peter T. , Simmons Daniel T. 
Published: February 2007

AbstractThe cellular tumor antigen p53 was isolated from mouse neuroblastoma cells and was found in a form not complexed to another protein. The p53 in these cells was stable, turning over about every 10 h. Its methionine‐labelled tryptic peptides were very similar to those of the p53 isolated from SV40‐transformed mouse cells. The labelled protein was purified from neuroblastoma cells by immunoaffinity using specific monoclonal antibodies and was about 80% radiochemically pure. Furthermore, the purified p53 sedimented in sucrose gradients with a sedimentation coefficient of approximately 8S. This correlated with the sedimentation coefficient of p53 prior to purification, showing that the purified protein retained its native size.
Increase in homotypic aggregation of metastatic morris hepatoma cells after fusion with membranes from non‐metastatic cells
Authors: Hausman R. E. 
Published: February 2007

AbstractThree types of Morris hepatoma: 7288C, 5123tc, and 9618A were assessed for their ability to grow and produce metastases in the lungs of male buffalo rats. Hepatoma 7288 formed metastatic growths more rapidly than 5123, while 9618 did not form metastatic growths in the lung at all. Cells from the same three hepatomas were then assessed for their ability to aggregate homotypically in vitro. This was used as a measure of potential for detachment from the site of primary growth in vivo, one of the critical steps in metastasis. The results were that 9618 aggregated more effectively than 5123 and 7288 did not aggregate homotypically. Thus, the ability to metastasize and the ability to aggregate homotypically were inversely related in these three tumors, with the most metastatic tumor, 7288, hardly aggregating at all. Was the ability of hepatoma 9618 to aggregate homotypically and the inability of hepatoma 7288 to do so a characteristic of the plasma membrane? Plasma membranes either from the tumor which aggregated best, 9618, or from the liver of non‐tumorous neonatal buffalo rats were fused into the surface of the 7288 cells. Either fusion conferred the ability to aggregate on the previously non‐aggregating metastatic hepatoma cells. In contrast, fusion of plasma membranes from hepatoma 7288 into 9618 cells failed to affect their ability to aggregate homotypically. The aggregation‐conferring effect required actual fusion of the donor membrane, was dependent on the amount of membrane fused and was sensitive to treatment of the donor plasma membranes with trypsin. The results are interpreted as suggesting the presence of homotypic aggregation materials, probably proteins, in the plasma membranes of non‐metastatic cells. These may be reduced or absent in the membrane of the highly metastatic hepatomas.
Gamma‐interferon (IFN‐γ) produced during effector and target interactions renders target cells less susceptible to NK‐cell‐mediated lysis
Authors: Grönberg Alvar , Kiessling Rolf , Masucci Giuseppe , Guevara Luis A. , Eriksson Eréne , Klein George 
Published: February 2007

AbstractHuman mitomycin C‐treated PBL were mixed with cells of an NK sensitive hybrid cell line (PUTKO‐I). A fraction of tumor cells survived this treatment and could be recovered from the cultures. These surviving cells were completely NK‐resistant and this property persisted for 2–3 weeks after cultivation in fresh medium. Treatment of a clone (C13) of PUTKO‐I with PBL‐PUTKO mixed lymphocyte‐tumor‐cell culture (MLTC) supernatants resulted in a marked reduction in NK sensitivity after 8–12 h of treatment. The kinetics of induction of NK resistance by MLTC supernatants was similar to that of purified IFN‐γ and was faster than for IFN‐α. The active component in the supernatants was characterized as a mixture of IFN‐γ and IFN‐α based on neutralization of activity with specific antisera. The role of mycoplasma contamination was investigated and it was found that cell lines free of detectable mycoplasma stimulated production of NK‐protective activity by PBL and this activity was neutralized by anti‐IFN‐γ serum. Separation of PBL on discontinuous Percoll gradients demonstrated a correlation between the NK activity of cell fractions and their ability to produce IFN in response to tumor cells. Taken together, the selection‐dependent variations in NK sensitivity, the kinetics of IFN production and induction of resistance suggest that tumor cells may be able to escape elimination by NK cells due to protection by IFN produced by the effector‐cell‐containing population.
A 3q+ marker chromosome in EBV‐carrying nasopharyngeal carcinomas
Authors: Mitelman F. , Mark‐Vendel E. , Mineur A. , Giovanella B. , Klein G. 
Published: February 2007

AbstractThe malignant epithelial cells of two anaplastic EBV‐carrying nasopharyngeal carcinomas (NPC) were investigated cytogenetically by Giemsa banding technique. Both tumors had a similar 3q+ marker chromosome with an involvement of band q25, either a dupliction of the region q25–q27, or insertion of an unidentified segment at band q25.
Characterization of malignant and non‐neoplastic cell phenotypes in highly malignant non‐Hodgkin lymphomas
Authors: Porwit‐Ksiazek Anna , Christensson Birger , Lindemalm Christina , Mellstedt Håkan , Tribukait Bernhard , Biberfeld Gunnel , Biberfeld Peter 
Published: February 2007

AbstractMalignant and non‐neoplastic cells in 38 cases of highly malignant non‐Hodgkin lymphomas: 3 centrocytic anaplastic, 18 centroblastic, 13 immunoblastic and 4 lymphoblastic (according to the Kiel classification) were immunophenotyped in cryosections and cell suspensions by means of monoclonal antibodies. Additionally, cell cycle analysis on cell suspensions was performed by DNA flow cytofluorometry. In 33 (87%) lymphomas the malignant cells expressed monoclonal surface immunoglobulin (Ig), which indicated B‐cell origin of tumors. In 7 of the 19 B‐cell lymphomas tested by the peroxidase‐antiperoxidase method, cytoplasmic Ig was found. Four lymphomas were of T‐cell and one of non‐B/non‐T‐cell origin. In 11 B‐cell and 2 lymphoblastic non‐B‐cell tumors, common acute lymphobiastk leukemia antigen (CALLA) was found. In 25 of 30 studied NHL the mafignant cells expressed receptor for transferrin and in 19 of 28 cases a high percentage of cells in S‐phase (> 10.85%) was found. Number and distribution as well as type of non‐B‐cell infiltrating B‐cell‐derived lymphomas varied considerably from case to case. Among these cells Leu 3+ (T heloer/inducer) cells predominated. Leu 2+ (T suppressor/cytotoxk) and Leu 7+ (natural killer and killer) cells constituted less numerous groups. Correlation of cytodiagnostic analyses with clinical observations indicates that high content of infiltrating T cells may be a favorable prognostic feature in highly malignant B‐cell lymphomas.
Prognostic implications of karyotype and morphology in patients with non‐Hodgkin's lymphoma
Authors: Kaneko Yasuhiko , Rowley Janet D. , Variakojis Daina , Haren J. Marie , Ueshima Yoshimi , Daly Karen , Kluskens Larry F. 
Published: February 2007

AbstractClonal chromosome abnormalities were observed in 30 patients with non‐Hodgkin's lymphoma; the type of lymphoma was characterized on the basis of the International Working Formulation. The 30 patients were classified into five groups according to the chromosome abnormality. There were 8 patients with t(14;18), 3 with t(8;14), 7 with a translocation to the long arm of chromosome 3 (a 3q+ chromosome), 5 with near‐tetraptoidy, and 7 with other abnormalities. Among the 8 patients with t(14;18), 5 had follicular small cleaved‐cell lymphoma (FSC), I had follicular mixed cell lymphoma (FM), and 2 had diffuse large‐cell lymphoma (DL); the diagnosis in these 2 patients was based on extranodal tissue. All 3 patients with t(8;14) had DL and B‐cell markers. Except for I patient, all those with a 3q+ chromosome had DL; 4 of those who were tested had B‐cell or pre‐B‐cell markers. Four of the 5 patients with near‐tetraploidy had follicular mixed‐cell lymphoma, and 2 of the 7 patients with other abnormalities had T‐cell lymphoma. Thus, patients with a t(8;14), a 14q+ chromosome, or a 3q+ chromosome all tend to have diffuse large‐cell lymphoma, usually of the non‐cleaved type. On the other hand, our data suggest that patients with FSC generally have a t(14;18) whereas those with follicular and diffuse mixed small cleaved cells and large non‐cleaved cells have a different pattern with modal chromosome numbers in the tetraploid range. We added 17 previously reported patients to the 30 presented here and correlated the karyotype with survival. The 6 patients with near‐tetraploidy had the longest median survival, 69 months, the 15 patients with t(14;18) had the next longest, 48 months. The 4 patients with (8;14) had Che shortest urvival, 12 months, and the 9 with other abnormalities had the next shortest, 17 months. Intermediate survivals of 27 and 30 months were observed in patients with a 14q+ or a 3q+ chromosome, respectively. The median survival of thaw various categories differs and our data, thus, indicate that the karyotypic pattern of the malignant cell may be a significant independent prognostic feature influencing the survival of patients with non‐Hodgkin's lymphoma.
Establishment and characterization of a human colon carcinoma cell line (KMS‐4) from a patient with hereditary adenomatosis of the colon and rectum
Authors: Namba Masayoshi , Miyamoto Kanji , Hyodoh Fuminori , Iwama Takeo , Utsunomiya Joji , Fukushima Fujiko , Kimoto Tetsuo 
Published: February 2007

AbstractA human colon carcinoma cell line was established from a metastatic lymph node of a patient with hereditary adenomatosis of the colon and rectum (ACR). Cells of this line, designated KMS‐4, have been continuously propagated in culture during the past 24 months. The cells growing on the surface of culture dishes showed epithelial features, and, when inoculated into athymic nude mice, produced adenocarcinomas with a morphology similar to that of the original tumor. Electron micrographs showed that the cultured cells have desmosomes and numerous surface microvilli typical of colon epithelium. Chromosomal analysis revealed the cell line to be of human origin with a diptoid mode of chromosome number, but the karyotypes examined were all abnormal. Most of the metaphases Commonly had such abnormalities as 7p+, 12p+, +13, +16 and 17p+, accompanied by loss of chromosome No. 19 and/or 20, and, interestingly, all the metaphases contained 7p+ and +13. The cells had a log phase doubling time of 48 to 72 h. The cloning efficiency of the cells was 0.06% in the soft agar medium. Neither 12‐0‐tetradecanoytphorbol‐13‐acetate nor bile acids enhanced cell proliferation. The cells abundantly secreted CEA protein into the culture medium (700 ng/ml/106 cells during a 48 h period). The present colon carcinoma cell line derived from a genetically defined individual with ACR should prove useful for research in human oncology or genetics.
A pigmentation‐associated, differentiation antigen of human melanoma defined by a precipitating antibody in human serum
Authors: Mattes M. Jules , Thomson Timothy M. , Old Lloyd J. , Lloyd Kenneth O. 
Published: February 2007

AbstractAntibodies in the serum of melanoma patient AU precipitate an antigen from 125I‐labelled extracts of cultured autologous melanoma cells. The antigen, which is probably not a cell surface component, is present in other pigmented melanomas but not in non‐pigmented melanomas or other tumor cell types, and the amount of antigen is correlated with the degree of pigmentation. These conclusions are based on absorption experiments with 11 pigmented melanomas, 8 non‐pigmented melanomas, 3 astrocytomas, 12 carcinomas of various histological types, 1 leukemia, 2 EB‐virus‐transformed B lymphocyte lines, and human erythrocytes. The antigen was also detected in cultured human melanocytes. It has a molecular weight of 70,000, an isoelectric point of pH 5.3, and it binds to concanavalin A‐Sepharose. Ninety‐six sera from other melanoma patients were examined and none of them precipitated this antigen. As described previously, the serum from patient AU also has antibodies to a unique (Class 1) tumor antigen found only on AU melanoma cells. The pigmentation‐associated, differentiation antigen and the unique antigen are clearly different in their distribution, but some relationship between these unusual antibody responses is possible.
A prospective study of urinary androgen levels and ovarian cancer
Authors: Cuzick Jack , Bulstrode J. C. , Stratton I. , Thomas B. S. , Bulbrook R. D. , Hayward J. L. 
Published: February 2007

AbstractThe three major urinary androgen metabolites, dehydroepiandrosterone (DHEA), androsterone (ANDRO), and aetiocholanolone (AETIO) were measured in 1,484 volunteer women between 29 and 60 years of age on the island of Guernsey from 1962–1967. Twelve of these women subsequently developed ovarian cancer after a median interval of 130 months and a minimum interval of 19 months. All three androgen levels in these women were lower than those in controls matched for age and menopausal status. The results were most striking for DHEA, where half of the cases were below the 27th percentile of their matched controls (p = 0.007, two‐sided). The results for ANDRO were of marginal significance (p = 0.06), and those for AETIO were not significant (p= 0.033).
Dietary cholesterol and lung cancer risk in a multiethnic population in Hawaii
Authors: Hinds M. Ward , Kolonel Laurence N. , Hankin Jean H. , Lee James 
Published: February 2007

AbstractWe interviewed 364 subjects with lung cancer and 627 age‐ and sex‐matched controls among the multiethnic population of Hawaii. Quantitative estimates of dietary cholesterol and vitamin A intakes were obtained, as well as smoking and occupational histories. Using multiple logistic regression analysis to control for potential confounding variables, we found a monotonic positive dose‐response relationship between dietary cholesterol and lung cancer risk in all subjects, in smoking subjects, and in men. In each of these groups, subjects in the highest quartile of dietary cholesterol were at significantly increased risk of lung cancer (lower 95% confidence limit >1.0). Among females, however, no clear association of dietary cholesterol with lung cancer risk was apparent.
Protein a treatment of cancer: Activation of a serum component with trans‐species anti‐B16 melanoma activity
Authors: Cooper Peter D. , Masinello Gillian R. 
Published: February 2007

AbstractMice (C57BL) succumbed to cultured B16 melanoma cells i.p. with reproducible kinetics and an MST2 of about 26 days. Serum from tumour‐bearing or normal mice was treated at 0°C with fixed SAC cells and injected i.p. into fresh tumour‐bearing mice. If serum was given 7 days or less after B16 inoculation, the MST of the mice was highly significantly increased by up to 32%. Similar activity has been generated in normal human, rabbit and guinea‐pig serum, while untreated sera were ineffective. Apparently the sera contained an inactive native precursor that was activated by the SAC to produce an anti‐tumour agent. Precursor and product were both relatively labile at 0°C. Anti‐tumour activity was eluted at pH 2.5 from SAC or Sepharose‐protein‐A pretreated with serum, thus implicating the protein A component of SAC. The eluates contained haemolytically active Cl, the first component of complement, and five crude Cl preparations made by standard methods showed good anti‐tumour activity. However, seven other highly haemolytic Cl preparations had no anti‐tumour effect. Similarly, two crude preparations of the subcomponent Clq had good anti‐tumour activity, but eight other, more pure and highly haemolytic Clq preparations were inactive in mice. Thus the antitumour principle was not Cl or Clq alone, although it had some chemical properties in common with these substances. It remains unidentified, but has potential interest for cancer therapy.
Enhancement or inhibition of tumor growth by interferon: Dependence on treatment protocol
Authors: Murasko Donna M. , Fresa Kerin , Mark Raymond 
Published: February 2007

AbstractMSC cells are tumor cells originally induced in BALB/c mice by Moloney sarcoma virus. In these studies we demonstrated that, although these tumor cells are sensitive in vitro both to lysis by NK or NK‐like cells and to the growth‐inhibitory effect of murine L‐cell interferon (IFN), the growth of the tumor in vivo could be either inhibited or enhanced by IFN. The outcome of in vivo IFN treatment was dependent on the timing and route of IFN administration relative to tumor challenge. IFN given systemically at the same time as tumor challenge resulted in enhancement of primary tumor formation, rate of tumor growth and subsequent progressive tumor growth. In contrast, IFN administered at the site of tumor inoculation on days 1–3 after tumor challenge inhibited tumor formation and growth. Histopathology of tissue sections obtained from the site of tumor challenge confirmed these results. Similar studies performed in mice given 450 rads of X‐irradiation showed that IFN could still inhibit tumor growth when administered at the site of tumor inoculation on days 1‐3 after tumor challenge. IFN administered simultaneously with tumor challenge, however, did not enhance tumor growth in irradiated mice. These results are consistent with the interpretation that 1) inhibition of MSC‐induced tumor growth by IFN has a radioresistant component and 2) the enhancement of MSC‐induced tumor formation by IFN is dependent on interaction with a radiosensitive population of cells, possibly lymphoid cells.
Association between susceptibility to dibenzanthracene‐induced fibrosarcoma formation and the Ah locus
Authors: Kour Richard E. , Connolly Geralynn M. , Nebert Daniel W. , Lubet Ronald A. 
Published: February 2007

AbstractThe relationship between the Ah locus and the induction of subcutaneous fibrosarcomas by dibenz[a,h]anthracene and dibenz[a,c]anthracene was investigated in C57BL/6J (Ahb/Ahb), (C57BL/6J)(DBA/2J)F1 (Ahb/Ahd) and (Ahd/Ahd) mice. Ahb/Ahb and Ahb/Ahd mice have the high‐affinity Ah receptor and therefore the polycyclic hydrocarbon induction of aryl hydrocarbon hydroxylase activity (cytochrome P1‐450) proceeds with ease; Ahd/Ahd mice have the poor‐affinity Ah receptor and this induction process proceeds more poorly, by a factor of at least 10‐fold. Dibenz[a,c]anthracene proved to be a relatively weak carcinogen, producing less than 3% tumor incidence at doses up to 300 μg per mouse. In contrast, dibenz‐[a,h]anthracene caused an almost 50% tumor incidence in Ahb/Ahb and Ahb/Ahd mice, while causing ∼2% tumor incidence in Ahd/Ahd mice. Both isomers bind avidly to the cytosolic Ah receptor, and both chemicals induce aryl hydrocarbon hydroxylase activity in Ahb/Ahb and Ahd/Ahd animals. Among progeny of the (C57BL/6J) (DBA/2J) F1 × DBA/2J backcross, 63 of 100 Ahb/Ahd mice and none of 75 Ahd/Ahd mice developed tumors. These data demonstrate a strict correlation between susceptibility to dibenz[a,h]anthracene‐induced subcutaneous tumors and expression of the Ahb allele, i.e. presence of the high‐affinity Ah receptor and therefore readily inducible P1‐450.