bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibodies reacting with human T‐lymphotropic retrovirus (HTLV‐1) or related antigens in lymphomatous and healthy hamadryas baboons
Authors: Voevodin A. F. , Lapin B. A. , Akovleva L. A. Y , Ponomaryeva T. I. , Oganyan T. E. , Razmadze E. N. 
Published: February 2007

AbstractThe sera of lymphomatous and healthy hamadryas baboons of the main, lymphoma‐prone Sukhumi stock were tested for antibodies reacting with HTLV‐1 antigens in the indirect immunofluorescence test. Antibodies of this specificity were found in all but one of 58 lymphomatous baboons and in 45% of 177 healthy ones. The prevalence of HTLV‐1 reactive antibodies in lymphoma‐free baboon populations (including 118 Sukhumi „forest”︁ stock animals and 195 baboons imported in 3 groups from Ethiopia) was consistently lower (5–8%). The specificity of baboon antibodies reacting with HTLV‐1 or a related agent is supported by the following evidence: (1) Concordant reactivity pattern of baboon sera with several HTLV‐1‐positive and ‐negative human cell lines; (2) elimination of baboon sera anti‐HTLV reactivity by absorption with purified HTLV‐1, but not by other retroviruses; (3) significant correlation between immunofluorescence titers of baboon sera and their reactivity in enzyme‐linked immunosorbent assay with purified HTLV‐1; (4) competition of baboon anti‐HTLV with monoclonal antibodies GIN‐14 for binding of the epitope on p19HTLV‐1. The prevalence of anti‐HTLV positives in the main Sukhumi stock increased by age, reaching its maximum (approx. 80%) at 5–15 years, and showed no significant sex‐related variation. The level of anti‐HTLV antibodies in lymphomatous baboons and in age‐, sex‐ and population‐matched healthy ones did not differ. However, in pre‐lymphoma sera these antibodies reached significantly higher levels than in sera of lymphomatous baboons (obtained in the terminal stage) or of matched, healthy controls.
Differential enhancement of melphalan cytotoxicity in tumor and normal tissue by fluosol‐DA® and oxygen breathing
Authors: Teicher Beverly A. , Holden Sylvia A. , Rose Christopher M. 
Published: February 2007

AbstractThe addition of Fluosol‐DA® carbogen breathing to melphalan treatment of the FSa‐IIC fibrosarcoma was assessed by tumor growth delay and cell survival assays. Melphalan (10 mg/kg) administered intraperitoneally (i.p.) was preceded by Fluosol‐DA (0.3 ml) administered intravenously (i.v.) and followed by 1 hr of carbogen breathing; this resulted in a tumor growth delay of 9.5 ± 1.4 days or an approximately 3‐fold increase compared to melphalan alone. Melphalan produced about 1.7 logs of cell killing; neither carbogen breathing nor Fluosol‐DA pretreatment altered the cell killing observed. There was a 10‐fold increase in tumor‐cell killing when Fluosol‐DA was administered immediately prior to melphalan administration followed by carbogen breathing for 1 hr. Density gradient separation identified a population of denser FSaIIC cells which showed increased sensitivity to melphalan after Fluosol‐DA administration. There was no additional toxicity to bone marrow as measured by CFU‐GM with the combination of melphalan/Fluosol‐DA/O2 compared to melphalan alone.
Glycoconjugates of murine tumor lines with different metastatic capacities. II. Diversity of glycolipid composition
Authors: Schwartz Reinhard , Kniep Bernhard , Müthing Johannes , Mühlradt Peter F. 
Published: February 2007

AbstractA syngeneic tumor system in DBA/2 mice consisting of a methyl‐cholanthrene‐induced, weakly metastatic lymphoma, L5178YE (= Eb), its spontaneous strongly metastatic variant, L5178YES (= ESb), and an unrelated, methylcholanthrene‐induced, metastasizing tumor, MDAY‐D2, were used to study the relationship between metastatic behavior and composition of GSLs. The D‐I‐14C galactose and D‐I‐14C glucosamine‐labelled neutral GSL and gangliosides of these tumor cells, and additionally ConA‐stimulated spleen T cells from normal mice, were analysed by thin‐layer chromatography. Unlabelled GSLs of the tumors were also characterized by (HPLC) after perbenzoylation. Results obtained with the radioactively labelled GSLs correlated with those of the HPLC analysis of unlabelled GSLs. All tumors contained neutral GSL of the ganglio‐series. Weakly metastatic tumor Eb showed neutral GSL patterns comparable to those from ConA‐stimulated spleen cells, whereas strongly metastatic tumors ESb and MDAY‐D2 had an enhanced expression of lactosylceramide. Gangliosides of metastatic ESb and MDAY‐D2 had a higher degree of polarity than those of weakly metastatic Eb. Eb cells expressed primarily GM1. Metastasizing ESb and MDAY‐D2 had significantly higher amounts of GM3, GM2 and GD1a. An unusual ganglioside, IV3GalNAc‐GMI, was found in MDAY‐D2 cells and ConA blasts. When the extent of label was compared in neutral GSLs and gangliosides, metastasizing ESb and MDAY‐D2 were more heavily labelled in the ganglioside fraction (62%, 58%) than Eb (39%). ESb and MDAY‐D2 also contained larger amounts of gangliosides than Eb.
Extracellular material secreted by human colonic adenocarcinoma cell lines promotes spreading in serumfree medium and induces neurite outgrowth of PC‐12 cells
Authors: Bellot F. , Luis J. , El Battari A. , Secchi J. , Pichon J. , Cau P. , Marvaldi J. 
Published: February 2007

AbstractA completely defined medium has been designed to promote cell proliferation of 2 colonic adenocarcinoma cell lines of epithelial origin (HT 29 and HRT 18). The spreading of both cell types, especially of HT 29 cells, was not possible in a serum‐free medium supplemented with growth factors. Spreading was obtained in a defined medium (a I/I mixture of DMEM and F12 media supplemented with 5 μ g/ml transferrin, 5 ng/ml EGF, 10 ng/ml selenite and 15 mM HEPES PH 7.3) with an extracellular matrix‐like material (ECM) secreted by the cells themselves. The properties of the ECM have been studied: (I) ECM secreted by the 2 cell lines induced very quick spreading of HT 29 and HRT 18 cells (1 to 2 hr vs. 12 to 24 hr in serum‐supplemented medium). (2) ECM induced morphological differentiation of a rat pheochromocytoma cell line (PC 12). PC‐12 cells grown under these conditions began to develop neurite extensions as early as 2 hr after seeding.
Premature chromosome condensation: Evidence for in vivo cell fusion in human malignant tumours
Authors: Kovacs Gyula 
Published: February 2007

AbstractPremature chromosome condensation (PCC) was studied in direct preparations of tissues from patients with haematological diseases and carcinomas of various histological types. PCC was found in 6 out of 166 malignancies (128 haematological cases, 35 carcinomas and 1 malignant effusions) analysed with the GTG‐technique. Chromosome analysis revealed S‐phase and G1‐phase PCC in each case; the frequency of PCC varied between 1, 4 and 8.6% of the metaphases analysed. It is suggested that PCC chromosomes, which represent cell fusion in vivo, are not very rare in naturally‐occurring human malignancies. and that cell fusion may affect the malignant phenotype. In conjunction with other factors they may also explain the heterogeneity of tumour cell populations.
The diagnostic value of a hepatoma‐specific band of serum gamma‐glutamyl transferase
Authors: Xu Ke‐Cheng , Meng Xian‐Yong , Shi Yi‐Cai , Ge Zheng‐Ju , Ye Ling , Yu Zhi‐Jian , Yang Da‐Min 
Published: February 2007

AbstractUsing a vertical slab electrophoretic apparatus loaded with polyacrylamide stage gel plate, we observed a total of II bands of gamma‐giutamyl transferase (EC 2.3.2.2, GGT) on a gel plate and designated these GGT I‐XI by order of distance beginning at the positive pole. Positive GGT II was found in 81(90%) of 90 cases of primary hepatic carcinoma (PHC), in 9(90%) of 10 cases of secondary hepatic carcinoma (SHC), and in only 4 (3.1 %) of a total of 128 cases of acute and chronic liver disease. There was one case with positive GGT II among 45 cases of cancer of ampulla of Vater, but the possibility of hepatic metastasis could not be excluded. No GGT II was found in a total of 250 healthy persons and 21 pregnant women. PHC patients with negative alpha‐fetoprotein (AFP) had a positive GGT II rate of 84.6%, whereas in those with negative GGT II the positive rate for AFP was only 44.4%.
Human T‐cell leukemia virus type I: Pseudotype neutralization of Japanese And American isolates with human and rabbit sera
Authors: Hoshino H. , Miwa M. , Clapham P. R. , Weiss R. A. , Miyoshi I. , Yoshida M. 
Published: February 2007

AbstractCCC/2M, CCC/10Y and CCC/MT‐2 cat kidney cells producing Japanese isolates of human T‐cell leukemia virus type I (HTLVs) and HOS/PL human osteosarcoma cells producing an American isolate of HTLV were infected with vesicular stomatitis virus (VSV) to prepare VSV pseudotypes bearing envelope antigens of HTLVs. VSV propagated in CCC/2M cells contained plaque‐forming fractions that were not neutralized by treatment with anti‐VSV serum alone: VSV pseudotypes bearing envelope antigens of HTLV2M and CCC cat endogenous virus were formed by infection of CCC/2M cells with VSV. Japanese HTLV2M, HTLV10Y and HTLVMT‐2 and American HTLVPL pseudotypes were neutralized by sera of Japanese, American and British patients with ATL. Each serum, including the serum of the patient from whom HTLV2M or HTLV10Y had been derived, gave similar antibody titers against Japanese and American HTLV pseudotypes. The HTLV pseudotypes were also neutralized by rabbit serum raised against HTLVMT‐2. A rabbit antiserum against the C‐terminal half of the HTLV env protein produced in E. coli also neutralized Japanese and American HTLV pseudotypes. Thus, VSV pseudotype analyses indicated that envelope antigens of HTLVs represent a single serotype worldwide. The env protein produced in E. coli may be used to raise neutralizing antibody against HTLVs.
Monoclonal antibody (anti‐leu 7) directed against natural killer cells reacts with normal, benign and malignant prostate tissues
Authors: Wahab Zeinab A. , Wright George L. 
Published: February 2007

AbstractThe reactivity of monoclonal antibody (MAb) antileu 7 (HNK ‐ I) on formalin‐fixed sections of prostate tissues was determined by an immunoperoxidase assay. Anti‐Leu 7 was found to react with both primary and metastatic tissues from 6/6 normal prostate, 15/15 benign prostatic hyperplasia, and 37/39 prostate carcinoma samples. Anti‐Leu‐7 reactivity was localized in the cytoplasm of the supranuclear region of prostatic epithelial cells and the fibromuscular stroma did not stain. Myelinated nerve fibers in the stroma were stained with anti‐Leu 7 and this served as an internal control. Anti‐Leu 7 did not bind to prostatic acid phosphatase (PAP) or prostate‐specific antigen (PSA) in direct or competitive binding radioimmunoassays. Anti‐Leu 7 was more effective (5/5) in the identification of metastatic tumors of prostate origin than an MAb to PSA (2/5). The differential pattern of reactivity of anti‐Leu 7 compared to anti‐PSA on serial sections of prostate tissues suggests that Leu 7 may be a useful diagnostic and prognostic marker of prostate cancer.
The relationship between 17β hydroxysteroid dehydrogenase and breast tumour site and size
Authors: Beranek P. A. , Folkerd E. J. , Newton C. J. , Reed M. J. , Ghilchik M. W. , James V. H. T. 
Published: February 2007

AbstractThe activity of 17β hydroxysteroid dehydrogenase (17OHSD) was measured in adipose tissue adjacent to benign or malignant breast tumours. 17OHSD activity was signficantly correlated with tumour size in malignant tumours (r = 0.75) but not with benign tumours (r = 0.28). Enzyme activity was reduced in adipose tissue adjacent to tumours obtained from inferior breast quadrants.
Studies on components of immunotoxins: Purification of ricin and its subunits and influence of unreacted antibodies
Authors: Vidal H. , Casellas P. , Gros P. , Jansen F. K. 
Published: February 2007

AbstractTwo ricins were purified from the seeds of Ricinus communis by a simple method based on affinity chromatography allowing large‐scale preparations. Separation of these 2 ricins was achieved by ion‐exchange chromatography and studies of purified submits demonstrated that the 2 forms of ricin differed only in their B‐chains which showed widely differing isoelectric points. The A‐chains isolated from both ricins showed similar biological properties and contained 2 variants, A1 and A2, differing in their molecular weights and carbohydrate contents. These variants could be separated by affinity chromatography on Con‐A‐Sepharose which bound the A2 variant more tightly than A1. This property allowed us to obtain immunotoxin preparations devoid of free antibodies and to study the in vitro influence of free antibody on immunotoxin activity.
The rauscher‐MuLV‐induced leukemia, RBL‐5, bears two tumor‐associated transplantation antigens expressed on distinct molecules
Authors: Galetto Giorgio , Law Lloyd W. , Rogers Michael J. 
Published: February 2007

AbstractTumor cells frequently express on their surface a new antigenic determinant which renders them immunogenic in the host animal. When immunity to this antigen results in rejection of a syngeneic tumor transplant, it is referred to as a tumor‐associated transplantation antigen (TATA). RBL‐5 is a Rauscher murine leukemia virus (MuLV}‐induced leukemia of C57BI/6 origin that is potently immunogenic and shares a TATA with other tumors induced by the closely related Friend and Moloney‐MuLVs (FMR‐TATA). We have recently isolated a 175 kilodalton (kd) glycoprotein (gp175) which has all the properties expected of the FMR‐TATA (Rogers et al., 1984). When this molecule was separated from a purified total glycoprotein fraction by DEAE chromatography, the remaining glycoproteins still contained a highly immunogenic TATA. Control experiments involving radioimmunoassay and immunoprecipitation with rabbit anti‐gp 175 indicated that this immunogenicity was not due to residual gp 175 or breakdown products of gp 175. We therefore conclude that RBL‐5 expresses at least two distinct TATAs: gp175 and another glycoprotein distinguished from gp 175 by its elution from a diethylaminoethylcellulose (DE52) column. These results, from a completely in vivo system, support data with other tumors obtained by in vitro methods and indicate that tumor cells may express several immunogenic molecules.
Recognition with a monoclonal antibody of a cytoplasmic mammary carcinoma antigen, correlated to the estrogen receptor status
Authors: Krohn Kai , Helle Markku 
Published: February 2007

AbstractImmunohistochemical reactivity of mammary carcinomas with monoclonal antibodies (MAbs) to human milk fat globule (HMFG) membrane antigens was compared with the estrogen (ER) and progesterone receptor (PR) status of the tumors. Antibody III D 5 stained 55 of the 74 tumors studied, the reaction being of borderline intensity in 19 cases and unequivocally positive in 36 cases. The staining was always cytoplasmic; in addition occasional extracellular III D 5‐positive secretory material was observed. Positive reactivity of the tumor with antibody III D 5 was significantly correlated with ER and/or PR content of the tumors. The presence of extracellular, III D 5‐positive secretory material correlated to the ER but not to the PR status of the tumors.
Correlation of macrophage‐mediated tumor‐cell lysis with the production of macrophage cytolytic factor (CF). preliminary characterization of a factor inhibiting cf production
Authors: Gifford George E. , Loewenstein James , Yamin Aviva , Gallily Ruth 
Published: February 2007

AbstractMacrophage‐mediated cytolysis of thymidine‐prelabelled murine A9 fibrosarcoma cells was compared to the level of cytolytic factor (CF) present in the cultures by assaying supernatant aliquots on actinomycin (AcD)‐treated A9 fibrosarcoma cells. A good correlation between the level of A9 killing and CF titer was observed when different concentrations of lipopolysaccharide (LPS) were added to various macrophage populations: murine peritoneal cells, short‐term bonemarrow (BM)‐derived macrophages and JBMø macrophage lines. Optimal A9 killing and CF secretion, equivalent to the killing of about 1000 AcD‐pretreated A9 cells by a single macrophage, were obtained following activation of JBMø by LPS. CF production by BM‐derived macrophages was enhanced in serum‐free medium when compared to its release in the presence of fetal calf serum. The LPS‐activated macrophages could be restimulated by the activating agent to produce CF following one week of propagation in the absence of LPS. On the other hand, CF activity was absent from the supernatants of activated macrophages co‐cultured with normal embryonic fibroblasts, which are resistant to macrophage‐mediated killing. This effect could be attributed to a factor, secreted by normal fibroblasts but not by A9 cells, which suppressed CF release from the activated macrophages. Our data strongly support earlier observations, suggesting that CF [which appears to resemble the tumor necrosis factor (TNF)] is responsible for LPS‐induced macrophage‐mediated tumor cell lysis. It is suggested that suppression of the latter process by the fibroblast‐derived factor proceeds via inhibition of CF/TNF production from the macrophage.
Human tumor cells synthesize and secrete alpha‐2‐macroglobulin in vitro
Authors: Bizik Jozef , Vaheri Antti , Saksela Olli , Kalkkinen Nisse , Meri Seppo , Grófóva Marta 
Published: February 2007

AbstractIn previous studies we showed that human sarcoma and melanoma cell lines synthesize and secrete into culture medium a glycoprotein, migrating in urea sodium dodecyl sulfate‐polyacrylamide gel electrophoresis at Mr 140,000. It is not detected in cultures of the corresponding normal cells. Conditioned medium of the melanoma cell line HMB‐2, producing among the cell lines tested the largest amounts of this glycoprotein, has now been used as a source for purification of the protein. NH2‐terminal amino‐acid sequence determination of the purified glycoprotein showed that it is identical to human α2‐macroglobulin (α2M). Rabbit antibodies raised against the glycoprotein specifically reacted in immunoblotting and immunodiffusion tests with α2M present in human plasma. Likewise, these antibodies immunoprecipitated from the conditioned media of 35S‐methionine‐labelled melanoma and osteosarcoma cell lines the protein which had a molecular weight corresponding to α2M. α2M was also synthesized and secreted by 2 strains of fetal lung fibroblasts but not by fetal skin fibroblasts or adult skin fibroblasts autologous to the osteosarcoma cell line.
Difluoromethylornithine enhances inhibition of melanoma cell growth in soft agar by dexamethasone, clone a interferon and retinoic acid
Authors: Bregman Marvin D. , Meyskens Frank L. 
Published: February 2007

AbstractFive human melanoma cell lines (C8146C, C8161, C82‐7A, C83‐2CY and MIRW5) were shown to contain a significant number of melanoma colony‐forming units resistant to single‐agent treatment by dexamethasone, alpha‐interferon and trans‐retinoic acid. These biological modifiers were combined with difluoromethylornithine into a low‐dose combination using concentrations below pharmacologically achievable levels. The suppression of melanoma colony formation induced by this combination was consistent and significantly higher than that seen with any single agent, colony formation being reduced by an average of 90%. Leaving either DEX or DFMO out of the 4‐agent combination resulted in a significant decrease in the observed inhibition. This was also verified by the addition of putrescine which inhibited only the DFMO activity. Median effect analysis of the DFMO + IFN inhibition of C8161 cells demonstrated that the 2 agents interacted synergistically over the entire doseresponse curve. Of the high‐dose combination‐treated melanoma colony‐forming units, 97% did not form small growth units; most remained as arrested single cells, but the cells and small growth units could still metabolize tetrazolium stain after the experiment, suggesting that the high‐dose combination arrested the growth of the melanoma colony‐forming units via a non‐cytotoxic mechanism.
Autonomous proliferation of mewo human melanoma cell lines in serum‐free medium: Secretion of growth‐stimulating activities
Authors: Sauvaigo Sylvie , Fretts Ruth E. , Riopelle Richard J. , Lagarde Alain E. 
Published: February 2007

AbstractThe growth properties of a human melanoma cell line (MeWo) and of a variant (MeWo‐LCI) endowed with higher metastatic potential in nude mice were compared using hormonally‐defined serum‐free media. The two cell lines failed to arrest in Go following serum deprivation, and responded to INS and MSA but not to EGF, PDGF or FGF. Only MeWo‐LCI cells divided persistently in completely serum‐free medium, and formed a high percentage of spheroids in agarose supplemented or not with serum or individual growth factors. The conditioned media of serum‐free cultures of MeWo and MeWo‐LCI cells exhibited mitogenic activities. These were detected without prior concentration, fractionation or acid treatment. They stimulated DNA replication into sparse monolayers of autologous (MeWo, MeWo‐LCI) or homologous (SKMEL28 melanoma) cells and into NRK‐49F normal rat fibroblasts, and acted in synergy with INS in a dose‐dependent manner. Over a period of 5 days in culture, MeWo‐LCI cells produced bioactive material at a 2 to 3‐fold higher rate than MeWo cells, in both the absence and presence of INS. MeWo‐LCI‐conditioned medium promoted or enhanced colony formation of MeWo and NRK‐49F cells plated in serum‐free (± INS) agarose. The two cell lines expressed the same amount of NGF and EGF receptors. On the basis of these results we suggest that: (i) MeWo and MeWo‐LCI melanoma cells have obviated allocrine stimulation of the division process characteristic of normal cells by responding to their own mitogens; (ii) some of these mitogens are akin to TGFs; (iii) the less efficient synthesis of autostimulatory factors by MeWo cells may account for their weaker proliferative capacity in vitro, and possibly in vivo.
Interferon‐activated tumor inhibition in vivo. Small amounts of interferon‐gamma inhibit tumor growth by eliciting host systemic immunoreactivity
Authors: Giovarelli M. , Cofano F. , Vecchi A. , Forni M. , Landolfo S. , Forni G. 
Published: February 2007

AbstractTen international units (IU) of recombinant (r) or natural (n) murine interferon (MulFN)‐gamma were used for the in vivo immunotherapy of a chemically induced fibrosarcoma (CE‐2) of BALB/c mice. In vitro, doses of r‐IFN‐gamma below 100 units have a marginal antiproliferative effect on CE‐2 cells and do not induce expression of H‐2d class‐II antigens, whereas they do increase that of class‐I antigens. In vivo, 10 daily injections of 10 IU of r‐ or n‐MulFN‐gamma at the challenge site provide significant protection against increasing doses of CE‐2 tumor cells. This protection was enhanced when non‐reactive T‐lymphocytes from CE‐2 tumor‐bearing mice were admixed at a 10:1 ratio with the CE‐2 tumor cells. Combined lymphocyte and r‐MulFN‐gamma treatment also inhibited the growth of already established tumors when it was started before these reached a mean diameter of 5 mm. Tumor inhibition depends upon activation of the host immune system. The antitumor activity of r‐MulFN‐gamma and T‐lymphocytes was null when mice were first irradiated with 450 rads. Moreover, host leukocytes massively infiltrated the area of tumor growth and the small r‐MulFN‐gamma doses injected daily activated various host immunoreactivity mechanisms.