bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative characterization of tumor cell traction force on extracellular matrix by hydrogel microsphere stress sensor
Authors: Ma Xingquan , Wang Cong , Ji Changchun , Cao Xiaoshan , Dong Yuqing 
Published: February 2024

AbstractCell traction force (CTF) is a kind of active force that is a cell senses external environment and actively applies to the contact matrix which is currently a representative stress in cell–extracellular matrix (ECM) interaction. Studying the distribution and variation of CTF during cell–ECM interaction help to explain the impact of physical factors on cell behaviors from the perspective of mechanobiology. However, most of the strategies of characterizing CTF are still limited by the measurement needs in three‐dimensional (3D), quantitative characteristics and in vivo condition. Microsphere stress sensor (MSS) as a new type of technology is capable of realizing the quantitative characterization of CTF in 3D and in vivo. Herein, we employed microfluidic platform to design and fabricate MSS which possesses adjustable fluorescent performances, physical properties, and size ranges for better applicable to different cells (3T3, A549). Focusing on the common tumor cells behaviors (adhesion, spreading, and migration) in the process of metastasis, we chose SH‐SY5Y as the representative research object in this work. We calculated CTF with the profile and distribution to demonstrate that the normal and shear stress can determined different cell behaviors. Additionally, CTF can also regulate cell adhesion, spreading, and migration in different cell states. Based on this method, the quantitative characterization of CFT of health and disease cells can be achieved, which further help to study and explore the potential mechanism of cell–ECM interaction.
Enzyme Kinetics Analysis: An online tool for analyzing enzyme initial rate data and teaching enzyme kinetics
Authors: Mak Daniel A. , Dunn Sebastian , Coombes David , Carere Carlo R. , Allison Jane R. , Nock Volker , Hudson André O. , Dobson Renwick C. J. 
Published: February 2024

AbstractEnzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and cellular biology to pharmacology. However, because enzyme kinetics involves concepts rarely employed in other areas of biology, it can be challenging for students and researchers. Traditional graphical analysis was replaced by computational analysis, requiring another skill not core to many life sciences curricula. Computational analysis can be time‐consuming and difficult in free software (e.g., R) or require costly software (e.g., GraphPad Prism). We present Enzyme Kinetics Analysis (EKA), a web‐tool to augment teaching and learning and streamline EKA. EKA is an interactive and free tool for analyzing enzyme kinetic data and improving student learning through simulation, built using R and RStudio's ShinyApps. EKA provides kinetic models (Michaelis–Menten, Hill, simple reversible inhibition models, ternary‐complex, and ping‐pong) for users to fit experimental data, providing graphical results and statistics. Additionally, EKA enables users to input parameters and create data and graphs, to visualize changes to parameters (e.g., or number of measurements). This function is designed for students learning kinetics but also for researchers to design experiments. EKA (enzyme-kinetics.shinyapps.io/enzkinet_webpage/) provides a simple, interactive interface for teachers, students, and researchers to explore enzyme kinetics. It gives researchers the ability to design experiments and analyze data without specific software requirements.
An international multicenter study comparing COVID‐19 omicron outcomes in patients with hematological malignancies treated with obinutuzumab versus rituximab
Authors: Shafat Tali , Grupel Daniel , Porges Tzvika , Abuhasira Ran , Belkin Ana , Deri Ofir , Oster Yonatan , Zahran Shadi , Horwitz Ehud , Horowitz Netanel A. , Khatib Hazim , Batista Marjorie Vieira , Cortez Anita Cassoli , Brosh‐Nissimov Tal , Segman Yafit , Ishay Linor , Cohen Regev , Atamna Alaa , Spallone Amy , Chemaly Roy F. , Ramos‐Ramos Juan Carlos , Chowers Michal , Rogozin Evgeny , Oren Noga Carmi , Keske Şiran , Barchad Orit Wolfovitz , Nesher Lior ,   the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Respiratory Viruses (ESGREV)
Published: February 2024

AbstractObjectivesHematological malignancy (HM) patients treated with anti‐CD20 monoclonal antibodies are at higher risk for severe COVID‐19. A previous single‐center study showed worse outcomes in patients treated with obinutuzumab compared to rituximab. We examined this hypothesis in a large international multicenter cohort.MethodsWe included HM patients from 15 centers, from five countries treated with anti‐CD20, comparing those treated with obinutuzumab (O‐G) to rituximab (R‐G) between December 2021 and June 2022, when Omicron lineage was dominant.ResultsWe collected data on 1048 patients. Within the R‐G (n = 762, 73%), 191 (25%) contracted COVID‐19 compared to 103 (36%) in the O‐G. COVID‐19 patients in the O‐G were younger (61 ± 11.7 vs. 64 ± 14.5, p = 0.039), had more indolent HM diagnosis (aggressive lymphoma: 3.9% vs. 67.0%, p < 0.001), and most were on maintenance therapy at COVID‐19 diagnosis (63.0% vs. 16.8%, p < 0.001). Severe‐critical COVID‐19 occurred in 31.1% of patients in the O‐G and 22.5% in the R‐G. In multivariable analysis, O‐G had a 2.08‐fold increased risk for severe‐critical COVID‐19 compared to R‐G (95% CI 1.13–3.84), adjusted for Charlson comorbidity index, sex, and tixagevimab/cilgavimab (T‐C) prophylaxis. Further analysis comparing O‐G to R‐G demonstrated increased hospitalizations (51.5% vs. 35.6% p = 0.008), ICU admissions (12.6% vs. 5.8%, p = 0.042), but the nonsignificant difference in COVID‐19‐related mortality (n = 10, 9.7% vs. n = 12, 6.3%, p = 0.293).ConclusionsDespite younger age and a more indolent HM diagnosis, patients receiving obinutuzumab had more severe COVID‐19 outcomes than those receiving rituximab. Our findings underscore the need to evaluate the risk–benefit balance when considering obinutuzumab therapy for HM patients during respiratory viral outbreaks.
A meta‐analysis comparing efficacy and safety between proton beam therapy versus carbon ion radiotherapy
Authors: Jang Jeong Yun , Kim Kangpyo , Chen Miao‐Fen , Akimoto Tetsuo , Wang Michael Lian Chek , Kim Min‐Ji , Kim Kyunga , Lee Tae Hoon , Yoo Gyu Sang , Park Hee Chul 
Published: February 2024

AbstractBackgroundThis study aimed to compare the outcomes of proton beam therapy (PBT) and carbon ion radiotherapy (CIRT) by a systematic review and meta‐analysis of the existing clinical evidence.MethodsA systematic literature search was performed to identify studies comparing the clinical outcomes of PBT and CIRT. The included studies were required to report oncological outcomes (local control [LC], progression‐free survival [PFS], or overall survival [OS]) or adverse events.ResultsEighteen articles comprising 1857 patients (947 treated with PBT and 910 treated with CIRT) were included in the analysis. The pooled analysis conducted for the overall population yielded average hazard ratios of 0.690 (95% confidence interval (CI), 0.493–0.967, p = 0.031) for LC, 0.952 (95% CI, 0.604–1.500, p = 0.590) for PFS, and 1.183 (0.872–1.607, p = 0.281) for OS with reference to CIRT. The subgroup analyses included patients treated in the head and neck, areas other than the head and neck, and patients with chordomas and chondrosarcomas. These analyses revealed no significant differences in most outcomes, except for LC in the subgroup of patients treated in areas other than the head and neck. Adverse event rates were comparable in both groups, with an odds ratio (OR) of 1.097 (95% CI, 0.744–1.616, p = 0.641). Meta‐regression analysis for possible heterogeneity did not demonstrate a significant association between treatment outcomes and the ratio of biologically effective doses between modalities.ConclusionThis study highlighted the comparability of PBT and CIRT in terms of oncological outcomes and adverse events.
Fertility counselling and fertility preservation among early onset female cancer patients—A Finnish register‐based study
Authors: Melin Johanna , Holopainen Elina , Savolainen‐Peltonen Hanna 
Published: February 2024

AbstractIntroductionAdvances in multimodality cancer treatments have increased long‐term survival rates for early onset cancer patients, with 5‐year survival rates reaching 80% in Northern Europe. According to recent recommendations, clinicians should, as early as possible, inform cancer patients about the impact that cancer treatment may have on their fertility. Still, there is limited published data on fertility counselling (FC) and fertility preservation (FP) for cancer patients.MethodsThis register‐based study used hospital records to identify female cancer patients in the hospital district (n = 192) who received FC at the age of 16–42 years between 2011 and 2019.ResultsAltogether, 97 (50.5%) cancer patients were eligible for FP. Of these, 55 (56.7%) underwent FP, whereas 42 (43.3%) declined. Women undergoing FP were recommended cancer treatments with a higher risk of infertility (p = 0.01), and women with breast cancer were more prone to undergo FP than women with lymphoma (p = 0.043). In FP treatment cycles, the mean number of oocytes retrieved (13.9 ± 7.7 vs. 12.0 ± 6.5, p = 0.04) and transferrable embryos (4.7 ± 2.9 vs. 3.7 ± 2.8, p = 0.002) was higher among cancer patients compared to age‐matched comparisons with male or tubal factor infertility. The total mean gonadotropin dose used was higher among cancer patients (2243 ± 963 IU vs. 1679 ± 765 IU, p < 0.001).ConclusionWe conclude that a good ovarian response during FP can be achieved in female cancer patients.
Innovative Approaches to Sustainable Ammonia Synthesis under Mild Conditions
Authors: Castillejos Eva , García‐Bordejé Enrique 
Published: February 2024

AbstractAmmonia is one of the most important chemicals in the world because it is a feedstock for fertilizer production and, moreover, it has been proposed as a convenient storage media for renewable H2. Currently, it is produced by Haber‐Bosch process, which entails harsh operation conditions and a high carbon footprint, rendering the process difficult to be run by renewable energy. Therefore, substituting the conventional Haber‐Bosch process by other approach less energy‐intensive and carbon‐free is an urgent need. A milder process for ammonia synthesis will enable the implementation of smaller distributed plants more aligned with renewable energies. For this reason, there is a plethora of current research focusing on the development of suitable catalysts with higher activity and selectivity for nitrogen reduction and processes that will work under less severe operating conditions or even at ambient conditions. Some of the most relevant research approaches are here revised and compared.
Polymeric Carbon Nitride Nanosheets as a Metal‐Free Heterogeneous Catalyst for Highly Efficient Methanolysis of Polycarbonates
Authors: Xu Yanling , Ji Yi , Liu Yue , Deng Weihua , Huang Fei , Zhang Fan 
Published: February 2024

AbstractImproper end‐of‐life treatments of C−O‐linked plastics have caused serious environmental crises and resource waste. One effective method to solve this issue is the catalytic alcoholysis of these plastics. Traditional homogeneous catalysts or metal‐based heterogeneous catalysts for alcoholysis have some drawbacks, such as difficulty in complete separation, environmental threats, and economic burden. Thus, finding a green, low‐cost, recyclable, and highly efficient catalyst substitute is critical. Here, we reported that polymeric carbon nitride nanosheets can serve as a metal‐free heterogeneous catalyst for the methanolysis of polycarbonate, polyethylene terephthalate, and polylactic acid. The catalyst exhibits remarkable performance and could be reused at least four times. NHx was found to be the predominant active site of the catalyst by characterization and experiments. These results show promise for the design of metal‐free heterogeneous catalysts to depolymerize C−O‐linked plastics.
Internal Vanadium Doping and External Modification Design of P2‐Type Layered Mn‐Based Oxides as Competitive Cathodes toward Sodium‐Ion Batteries
Authors: Li Xin , Lai Xin , Kong Qingquan , An Xuguang , Zhan Jing , Li Xiaolei , Liu Xiaonan , Yao Weitang 
Published: February 2024

AbstractP2‐type layered manganese‐based oxides have attracted considerable interest as economical, cathode materials with high energy density for sodium‐ion batteries (SIBs). Despite their potential, these materials still face challenges related to sluggish kinetics and structural instability. In this study, a composite cathode material, Na0.67Ni0.23Mn0.67V0.1O2@Na3V2O2(PO4)2F was developed by surface‐coating P2‐type Na0.67Ni0.23Mn0.67V0.1O2 with a thin layer of Na3V2O2(PO4)2F to enhance both the electrochemical sodium storage and material air stability. The optimized Na0.67Ni0.23Mn0.67V0.1O2@5wt %Na3V2O2(PO4)2F exhibited a high discharge capacity of 176 mA h g−1 within the 1.5‐4.1 V range at a low current density of 17 mA g−1. At an increased current density of 850 mA g−1 within the same voltage window, it still delivered a substantial initial discharge capacity of 112 mAh g−1. These findings validate the significant enhancement of ion diffusion capabilities and rate performance in the P2‐type Na0.67Ni0.33Mn0.67O2 material conferred by the composite cathode.
Layer by Layer Spraying Fabrication of Aggregation‐Induced Emission Metal‐Organic Frameworks Thin Film
Authors: Yang Xue‐Xian , Li Chong , Chen Shu‐Mei , Gu Zhi‐Gang , Zhang Jian 
Published: February 2024

AbstractThe development of new metal‐organic frameworks (MOFs) thin films is important for expanding their functions and applications. Herein, we first report a new kind of MOF thin film by using aggregation‐induced emission (AIE) dicarboxyl ligand through a liquid‐phase epitaxial (LPE) layer‐by‐layer (LBL) spraying method (named AIE surface‐coordinated metal‐organic frameworks thin film, AIE‐SURMOF). The obtained AIE‐SURMOF Zn4O(TPE)3 (ZnTPE) has highly growth orientation and homogeneous thin film, showing strong fluorescent property. Furthermore, by loading chiral guest in the MOF pore, the formed chiral encapsulated AIE‐SURMOF can clearly indicate obvious circularly polarized luminescence performance with glum of 0.01. This study provides new MOF thin film and new strategy for expanding function and application of MOF materials.
Bioequivalence Assessment of Two Dapoxetine Hydrochloride Formulations in Healthy Chinese Males Under Fasted and Fed Conditions
Authors: Li Yumin , Zhang Zhen , Xie Jizhen , Lian Xianghua , Zhang Guangtao , Wang Cheng 
Published: February 2024

AbstractThis study evaluated the bioequivalence of the newly developed dapoxetine hydrochloride tablet relative to the marketed reference product by comparing their pharmacokinetic profiles under fasted and fed conditions. A total of 60 healthy Chinese male subjects participated in a single‐center, 2‐period, 2‐sequence, randomized, open‐label, self‐crossover study with a washout period of 14 days, 30 in the fasted group and 30 in the fed group. Following a single 30‐mg oral dose of the test or reference dapoxetine formulation, blood samples were collected before dosing to 72 hours after dosing. Liquid chromatography‐tandem mass spectrometry was performed to measure plasma concentration of dapoxetine and determine pharmacokinetic parameters through noncompartmental analysis. The vital signs and adverse events were also monitored during the study. The 90% confidence intervals of the geometric mean ratios for maximum plasma concentration, area under the plasma concentration‐time curve from time 0 to the last concentration time, and area under the plasma concentration‐time curve from time 0 extrapolated to infinity of the 2 dapoxetine formulations completely fell within the regulatory criteria for bioequivalence of 80%‐125%. In addition, both dapoxetine hydrochloride formulations were generally well tolerated. The generic dapoxetine hydrochloride tablet was bioequivalent to the marketed reference product in healthy Chinese men with no discernible safety differences.
Psychotherapist perceptions of engagement‐building behaviours with youth clients across developmental levels
Authors: Orlowski Edmund W. , Chen Jason I. , Breznik Lindsey H. , Gleason La Donna L. , Karver Marc S. 
Published: February 2024

AbstractBuilding a positive therapeutic relationship is a challenging, yet critical, first step in conducting youth psychotherapy. A number of studies in the youth treatment literature have indicated that a positive therapeutic alliance is related to increased treatment attendance, participation, and outcome. Some research has examined therapist behaviours for engaging therapy clients; however, developmental differences in alliance formation have had limited exploration. The current study surveyed clinicians about their use of specific engagement strategies and the developmental stage of their youth clients. It was hypothesised that participants would differentially rate the importance of different aspects of therapeutic engagement based upon a youth client's developmental stage and that these would correspond with differences in specific engagement strategies. A total of 64 clinicians with experience treating youth completed the study. The participants completed a questionnaire administered online that asked them to rate the importance of developmental differences to forming a therapeutic relationship and provide example client behaviours from their clinical experience for each developmental stage. Results showed clinicians felt the relative importance of collaboration, advocacy, and trustworthiness increased with age. These differences were also evidenced in the specific strategies clinicians endorsed in relation to each engagement factor across developmental stages. This program of research will eventually aid in the development of new guidelines for engaging clients in youth psychotherapy. In addition, the results may be used to enhance psychotherapy training for those working with children and adolescents.
A Bayesian approach using spatiotemporal features for suitable next hop selection in opportunistic networks
Authors: Dutta Amit , Borah Satya Jyoti , Singh Jagdeep 
Published: February 2024

SummaryOpportunistic network (OppNet) belongs to the category of Mobile Ad‐hoc Networks (MANETs), a kind of Delay Tolerant Network (DTN), where the wireless nodes are completely mobile and the data transmission routes are dynamic. The major challenge in developing a routing model for such a network is the unpredictable nature of the movement of the nodes. In this paper, a spatiotemporal prediction model based on human mobility pattern is proposed using Bayesian posterior probability (BPPR) where several clusters are identified within the network and the day and time duration of nodes visiting those clusters are recorded. The Bayesian posterior probability is then used to determine the probability of the neighbor node visiting the destination's cluster. If the calculated probability for that node is higher than a specified threshold, the packet will be forwarded. A comparison of the results obtained on simulation is made with benchmark models—Epidemic, Prophet, HBPR, EDR, NexT, and EBC, to name a few, where it is found that on average the proposed model outperforms the other models in terms of delivery probability, hop count and number of messages dropped by around 23.89%, 24.8%, 24.4%, 37%, 11%, and 42% respectively, with varying number of nodes, TTL, message generation interval, and buffer size. Similar improvements have been observed in terms of the other two metrics. In terms of overhead ratio, the proposed model outperforms Epidemic, Prophet, HBPR, NexT, and EBC. However, as the number of nodes and TTL are varied, BPPR performs better than NexT by around 9% and 12%, respectively, in terms of average latency.
Development of an indoor corridor localization system based on a WCL method with corridor area selection and distance compensation
Authors: Wattananavin Thradon , Booranawong Apidet , Saito Hiroshi 
Published: February 2024

SummaryIn this paper, a received signal strength indicator (RSSI)‐based weighted centroid localization (WCL) method for indoor corridor scenarios is developed and tested. The contribution of the proposed system is that, to scope the area of an unknown target position, the possible corridor area where the target node should be located is automatically selected, and only reference nodes deployed in such an area are applied for position estimation. Additionally, to improve the estimation precision, distance values converted from measured RSSIs and used for the WCL are also compensated to alleviate the RSSI variation problem caused by physical environments. Therefore, our methods can save a number of reference nodes to be used, while localization accuracy can also be achieved. Experiments in the corridor environment using a 2.4 GHz IEEE 802.15.4 wireless sensor network with four reference nodes deployed in the test field dimension of 22 m × 9.3 m have been performed. Experimental results demonstrate that the proposed method, which requires only two reference nodes, outperforms the original WCL method with four reference nodes by 53.053%, as indicated by average localization errors. The results also reveal that the proposed method provides error distances lower than the WCL method for all test target positions, while all estimated positions fall within the corridor areas by 100%.
Water discharge and sediment flux intermittency in the fluvial Escanilla Formation, Spain: Implications for changes in stratigraphic architecture
Authors: Sharma Nikhil , Whittaker Alexander C. , Adatte Thierry , Castelltort Sébastien 
Published: February 2024

AbstractWater discharge and sediment flux variations are important parameters controlling the morphodynamic behaviour of rivers. Although quantitative estimates for water discharge and sediment flux variability are well‐constrained for modern rivers, far fewer assessments of flow and sediment flux intermittency in ancient fluvial systems from the rock record are available. In this study, a relationship between water discharge, sediment flux variability and patterns of changing fluvial stratigraphic architecture in the Middle Eocene Escanilla Formation, Spain, is explored. Water discharge intermittency factor (IWF), calculated as a ratio of the total water discharge (over the averaging time period) to the instantaneous channel‐forming water discharge if sustained for the same period, ranges from 0.03 to 0.11 in the high amalgamation intervals and from 0.10 to 0.32 in the low amalgamation intervals. Similarly, the sediment flux intermittency factor (ISF) is estimated to be in the range of 0.008 to 0.01 in the high amalgamation intervals and of 0.01 to 0.03 in the low amalgamation intervals. Consequently, high amalgamation intervals were most probably deposited under more intermittent and short‐lived intense precipitation events while low amalgamation intervals were the result of less intermittent flows spread throughout the year. Overall, these estimates are consistent with values from modern ephemeral rivers typically found in arid to semi‐arid climate and is in agreement with available proxy data for the Middle Eocene climatic context of the studied alluvial system. This highlights an important connection between hydroclimate, river morphodynamics and landscape evolution, and has implications to predict river flow and sediment transport across the Earth's surface in the geological past.