bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Ozone, UV Radiation, Herbal and Glutaraldehyde Method for Sterilisation of Elastomeric Impression Materials for Orthodontic and Prosthodontic procedures
Authors: Ashil AM , Sharma Aseem , Faiz MK , Garhnayak Mirna , Mathar Mohammed Ibrahim , Anupama T , Mahalakshmi Gujjalapudi , Sharma Tanushree 
Published: February 2024

ABSTRACT

Objectives:
The objectives of the current study was to evaluate the disinfection efficacy of ozone, ultraviolet (UV) radiation, herbal and glutaraldehyde method for sterilization of elastomeric impression materials

Materials and Methods:
Disinfection of elastomeric impression material was performed with each of the disinfection methods: ozone, UV radiation, herbal, and glutaraldehyde. Later microbiological analysis was performed and the colony-forming units were evaluated and compared.

Results:
The highest disinfection efficacy was found with glutaraldehyde, followed by ozone and UV radiation, and the least with neem herbal rinse. Intergroup comparison was highly significant

Conclusion:
Dry gaseous ozone and UV radiation can be used effectively for the disinfection of impressions.
Assessment of Antibacterial Effectiveness of SDF and Fluoride Varnish Agents for Application in Pediatric Dentistry
Authors: Alessa Noura , Bidyasagar Bal Sourav Chandra , Beegum Fahanna , Ali Habibullah Mohammed , Farhan Mohammed , Almuntashri Ahmed Ali , Babaji Prashant 
Published: February 2024

ABSTRACT

Objectives:
To assess antibacterial effects of silver diamine fluoride (SDF) and fluoride varnish treatment against Streptococcus mutans and Lactobacillus acidophilus

Materials and Methods:
The antibacterial effectiveness of SDF (group A) and fluoride varnish (group B) against S. mutans was investigated in an in vitro microbiological investigation, with distilled water (group C) serving as the positive and negative controls. After 24 hours of incubation, the antibacterial efficiency was assessed using the agar well diffusion technique, and the diameter of the zones of inhibition (ZOI) was quantified. Sumba mare’s milk from MRS broth was extracted and then placed into a test tube. L. acidophilus was grown on Sumba mare’s milk from MRS broth. On this media, each testing agent was poured and tested for the inhibitory zone. The obtained data was statistically analyzed.

Results:
SDF group had a higher mean zone of inhibition against S. mutans and Lactobacillus followed by fluoride varnish, and there was no ZOI in the case of distilled water. Intergroup comparison was significant

Conclusion:
When compared to fluoride varnish, the SDF teeth remineralizing agent had greater antibacterial activity against S. mutans.
Sealing Ability Comparison of Three Different Obturating Systems to Intraradicular Dentin—An In Vitro Stereomicroscopic Study
Authors: Moinuddin Mohammed Khwaja , Ramachandruni Nimeshika , Tehreem Ayesha , Kasam Swetha , Anbalagan Sakthivel , Anupama N 
Published: February 2024

ABSTRACT

Background:
Different obturation techniques are being used to improve canal sealing, thereby minimizing marginal leakage.

Aims and Objectives:
To evaluate the marginal leakage of three different obturating materials and techniques using stereomicroscope

Materials and Methods:
Thirty freshly extracted maxillary anterior teeth were collected and were divided into three groups of 10 each for which the biomechanical preparation was done. The teeth were divided into three experimental groups according to the obturation material and technique used, Group I: gutta-percha with AH Plus root canal sealer (lateral condensation): Group II: thermoplasticized gutta-percha technique-non-carrier-based (calamus) with AH Plus sealer (backfill): Group III: C point (self-sealing root canal obturating system) with bioceramic sealer (single cone). Each specimen was subjected for testing apical sealing ability. The values of microleakage associated with different root canal sealers were evaluated using a stereomicroscope.

Results:
There was no statistically significant difference in the mean scores of apical dye penetration/leakage among all the three groups (P = 0.091), whereas Group I showed a higher degree of apical leakage than the other techniques tested

Conclusion:
None of the tested filling materials provided a gap-free or void-free root canal filling; however, thermoplasticized gutta-percha is used with AH Plus sealer exhibited less voids and gaps when compared to other tested materials.
Association of Positive Family History and Clinicopathological Features in Breast Cancer in Young Indian Females – A Pilot Study
Authors: Goyal Jaishree , Yadav Ajay , Malhotra Hemant 
Published: February 2024

ABSTRACT

Introduction:
Breast cancer is the most common cancer in Indian females, accounting for 31.8% of all cancers. Young women with breast cancer are those under 40 years of age. These patients have higher chances of positive family history and genetic susceptibility. Approximately 5%–10% of cases of breast cancer are associated with a family history. In this study, we planned to identify a relationship between family history and the clinical, pathological, and genetic characteristics of breast cancer in young women.

Methods:
Retrospectively, data from patients aged < 40 years with breast cancer, were collected from 2019 to 2022. Detailed information about the family history of the patients including the degree and number of relatives affected and the types of cancer was recorded. The tumors were characterized based on the pathological grade, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2 status, and BRCA variant genetic analysis.

Results:
Of 125 females with young breast cancer, 24 patients (19%) had a first- or second-degree relative with a history of cancer at the time of breast cancer diagnosis. Four (16%) of these positive cases had BRCA mutation detected. Bilateral breast cancer was detected in 12% of women with positive family history.

Conclusion:
Clinicians are urged to use detailed family histories for young women with breast cancer for early screening and education of the first- and second-degree relatives of these patients to detect the hidden cases.
The value of diagnostic vitrectomy: Histocytopathology techniques for the diagnosis of lymphoma of the retina
Authors: Provenzano Giovanna , Burnier Julia Valdemarin , Bergeron Sabrina , Youhnovska Emma , Marcotte Emily , Chen John , Sun Vincent , Burnier Miguel Noel 
Published: February 2024

Abstract

Purpose:
Primary large B-cell lymphoma of the retina, vitreous, and central nervous system (CNS), is an intraocular tumor with an unspecific and insidious clinical presentation. Pars plana vitrectomy (PPV) and optical coherence tomography (OCT) are useful diagnostic tools for this malignant process. The aim of this study is to evaluate the diagnostic efficacy of PPV for these intraocular lesions under a modified diagnostic protocol with a clinical pathological correlation with OCT imaging.

Materials and Methods:
A total of 115 samples were collected after a vitrectomy procedure (aspiration or vitrectomy cassette). The samples were centrifuged, and the precipitates were collected. A cell block was prepared and analyzed with multiple stains and an immunohistochemistry (IHC) panel, including B- and T-cell markers, as well as light chain markers, to establish the monoclonal nature of the tumor. Of the 115 samples, 9 (7.83%) were diagnosed with large B-cell lymphoma of the retina, vitreous, and CNS.

Conclusion:
Diagnostic vitrectomies for the large B-cell lymphoma of the retina, vitreous, and CNS is an excellent tool for the diagnosis of this entity. A negative diagnostic PPV with a strong suspicious OCT image, where the neoplastic cells are located between the retinal pigmented epithelium and Bruch’s membrane, the latter procedure should be either repeated or a chorioretinal biopsy be performed. In contrast, a positive vitrectomy using the IHC panel for large B-cell lymphoma of the retina, vitreous, and CNS is pathognomonic of this condition. In addition, the OCT is an important tool to help in the diagnosis of this difficult entity.

Results:
The diagnostic PPV provided suitable vitreous samples to all patients with undetermined uveitis and/or intraocular tumor suspicion. A morphological and immunohistichemical (IHC) analysis enabled a conclusive diagnosis of retina, vitreous and CNS lymphoma in all patients submitted to the procedure.
Diabetic papillopathy: A rare ocular manifestation of diabetes mellitus
Authors: Aguiar Catarina Pestana , Jesus Jeniffer Domingues , Chibante-Pedro João C. M. , Rocha António G. 
Published: February 2024

Abstract
Diabetic papillopathy (DP) is a rare ocular manifestation of diabetes mellitus. The exact etiology is not well understood, but in some cases, it may be associated with a rapid correction of the blood glucose level. There are limited case reports describing the use of intravitreal anti-vascular endothelial growth factor antibodies which have shown effectiveness in reducing disc swelling. In this case, we present the case of an 80-year-old diabetic woman diagnosed with DP during a diabetic retinopathy (DR) screening. Her visual acuity (VA) was counting fingers on her right eye and 20/160 on her left eye. At the fundoscopy, an exuberant bilateral hemorrhagic disc edema was observed, along with a nonproliferative DR. This edema was also evident in the fluorescein angiography and was documented through the optical coherence tomography. Brain computed tomography normal. She was treated with ranibizumab with significant improvement of the optic disc edema and the VA.
Efficacy and safety of chinese herbal medicines combined with chemical drugs for alzheimer's disease: A systematic review and meta-analysis
Authors: Xu Li , Chen Wen-Jun , Tian Cai-Jun , Zhang Yan , Ma Yan , Li Tian-Hao , Liu Hong-Jie , Zhang Zhe 
Published: February 2024

Objective:
To evaluate the efficacy and safety of Chinese herbal medicines (CHMs) combined with chemical drugs for the treatment of Alzheimer's disease (AD).

Materials and Methods:
Databases were searched to retrieve randomized controlled trials (RCTs) of CHMs combined with tacrine, galantamine, rivastigmine, donepezil, or memantine, following strict inclusion and exclusion criteria. Only research papers published in English, Chinese, and Japanese were considered. The primary outcome was the mini-mental state examination (MMSE) score and the secondary outcomes were AD assessment scale cognitive subscale (ADAS-Cog) score and safety evaluation. Meta-analysis was carried out using RevMan 5.3 and subgroup analysis was conducted to identify the sources of heterogeneity.

Results:
A total of 15 RCTs with 1386 participants were included in this study. Only donepezil was used in the retrieved literature. Meta-analyses showed that the combination of CHMs with donepezil led to significant improvement in the MMSE score (Z = 9.45; P < 0.00001; weighted mean difference [WMD] =2.68, 95% confidence interval [CI]: 2.12–3.24) and a significant decrease in the ADAS-Cog score (Z = 5.53; P < 0.00001; WMD = −3.79; 95% CI: −5.13–−2.44). Safety evaluation demonstrated that these combination treatments relieved adverse events such as insomnia (risk ratio [RR] =0.20, 95% CI: 0.06–0.68; P = 0.01) and hive (RR = 0.10; 95% CI: 0.01–0.73; P = 0.02).

Conclusions:
The combination of CHMs with a chemical drug like donepezil led to significant improvements in patient cognition as well as a better safety profile when compared to the application of a chemical drug alone.
Mechanism of mingjing granules in treating wet age-related macular degeneration based on network pharmacology and experimental verification
Authors: Li Xiao-Yu , Liang Li-Na , Zhang Wei-Jun , Gao Yun , Chen Qiang 
Published: February 2024

Objective:
To analyze the potential mechanism of Mingjing granules in the treatment of wet age-related macular degeneration (wAMD) based on the research methods of network pharmacology and molecular docking approach and to provide a new reference for the currently limited treatment of wAMD.

Materials and Methods:
We searched TCMSP, GeneCards, OMIM, PharmGkb, TTD, and DrugBank database to screen the main active ingredients of Mingjing granules and their therapeutic targets of wAMD. The network of active components and targets was constructed using Cytoscape3.6.1 software, which was also used for the topological analysis of target genes. The network of Protein-Protein Interactions (PPI) was mapped using the String platform. We also used R language to do the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for additional analysis. Molecular docking studies were finished by Chemoffice, Autodock, and Pymol. Finally, the efficacy of the Mingjing granules was examined in animal experiments, in which we used enzyme-linked immunosorbent assay to the contents of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) levels in peripheral blood.

Results:
Active compounds, including quercetin, lignocaine, and kaempferol, were found. PPI network analysis showed that tumor necrosis factor (TNF), MMP-9, epidermal growth factor (EGF), prostaglandin-endoperoxide synthase 2 (PTGS2), and caspase-3 (CASP3) were related to both Mingjing granules and wAMD. GO and KEGG pathway analysis showed that these targets were mainly involving lipids and atherosclerosis, TNF, and interleukin-17 (IL-17) signaling pathways. Docking studies suggested that quercetin and luteolin can fit in the binding pocket of four target proteins (CASP3, EGF, PTGS2, and TNF). In the vivo experiment, the Mingjing granules were found to be effective on the expression of VEGF and MMP-9 in peripheral blood.

Conclusions:
This study initially reveals the multi-constituent, multi-target, and multi-pathway mechanism of action of Mingjing granules in the treatment of wAMD and implies the inhibition of choroidal neovascularization may be related to the expression of VEGF and MMP-9.
Network pharmacology-based dissection of the bioactive compounds and pharmacological mechanisms of yiqi fumai lyophilized injection for the treatment of heart failure
Authors: Huang Yu-Xi , Fan Jing-Jing , Xu Lu-Lu , Yu Rong , Kuang Yi , Chai Yue , Zheng Rui , Zhang Xiao-Yu , Shan Hong-Cai , Qiao Xue , Ye Min 
Published: February 2024

Objective:
Yiqi Fumai Lyophilized Injection (YQFM), a Chinese medicine injection, has been widely used for the treatment of cardiovascular diseases, especially heart failure (HF). However, bioactive compounds and underlying mechanisms of YQFM in treating HF remain poorly understood.

Materials and Methods:
Network pharmacology was employed to investigate the bioactive compounds and mechanisms of YQFM. A compound-target network was constructed to screen bioactive compounds based on contribution index calculation. Then, an adriamycin-induced HF rat model was established to evaluate the cardio-protective effects of YQFM by hematoxylin and eosin staining and enzyme-linked immunosorbent assays.

Results:
Network pharmacology indicated that YQFM may alleviate HF through 36 compounds and 109 targets. Particularly, ginsenosides Rb1, Rg1, Re, Rf, Rb2, Rh1, schisandrin, and ginsenoside Rc were indicated as the top contributors of YQFM in treating HF. YQFM was predicted to act on multiple targets such as vascular endothelial growth factor A, interleukin-2 (IL-2), IL-6, and IL-1β, as well as to regulate signaling pathways such as hypoxia-inducible factor 1, tumor necrosis factor, VEGF, and PI3K-Akt. The pharmacological study suggested that YQFM could attenuate cardiac injury and up-regulate plasma concentrations of VEGFR-1 and NO in HF rats. Ginsenoside Rb1, as the major contributor from network pharmacology analysis, also showed a cardioprotective effect and up-regulation of VEGFR-1 in plasma.

Conclusions:
Ginsenosides and schisandrin were predicted as the most important contributors to the cardioprotective effect of YQMF. Ginsenoside Rb1 was proved to alleviate HF and increase the plasma concentration of VEGFR-1.
Effective Sato–Tate conjecture for abelian varieties and applications
Authors: Bucur Alina , Fité Francesc , Kedlaya Kiran S. 
Published: February 2024

From the generalized Riemann hypothesis for motivic

L

-functions, we derive an effective version of the Sato–Tate conjecture for an abelian variety

A

defined over a number field

k

with connected Sato–Tate group. By
effective
we mean that we give an upper bound on the error term in the count predicted by the Sato–Tate measure that only depends on certain invariants of

A

. We discuss three applications of this conditional result. First, for an abelian variety defined over

k

, we consider a variant of Linnik’s problem for abelian varieties that asks for an upper bound on the least norm of a prime whose normalized Frobenius trace lies in a given interval. Second, for an elliptic curve defined over

k

with complex multiplication, we determine (up to multiplication by a nonzero constant) the asymptotic number of primes whose Frobenius traces attain the integral part of the Hasse–Weil bound. Third, for a pair of abelian varieties

A

and

A'

defined over

k

with no common factors up to

k

-isogeny, we find an upper bound on the least norm of a prime at which the respective Frobenius traces of

A

and

A'

have opposite sign.
Cattle tick-borne pathogens sharing the same vectors in Benin and Burkina Faso: variations in prevalence and coinfection patterns
Authors: Biguezoton Abel S. , Adehan Safiou B. , Adakal Hassane , Badolo Honorine , Toure Mamadou , Zoungrana Sebastien , Farougou Souaibou , Chevillon Christine 
Published: February 2024

Understanding interactions between pathogens at the level of the individual host and the population in West Africa, may have noteworthy implications for predictions of diseases emergence and disease control programmes. Hence, the current study was aimed at investigating the interactions between Anaplasma marginale, Babesia bigemina and B. bovis in West African cattle. Twelve sentinel steers in each of the eight selected herds were randomly chosen to perform a one-year-long survey designed to monthly collect blood sample and to diagnose possible infections of the steers. This allowed identifying A. marginale as the most prevalent pathogen across the three surveyed regions (range: 0.60-1), followed by B. bigemina (0.24-0.85) and B. bovis (0.10-0.64); the same ranking order of the tick-borne pathogens was observed when considering the apparent duration of cattle infection. Regarding interaction patterns, mainly avoidance was revealed between A. marginale and B. bigemina and between B. bigemina and B. bovis. Such negative interaction seemed to be beneficial to the West African cattle surveyed, helping them to escape from babesiosis caused by B. bovis. Altogether, studied Boophilus tick-borne pathogens negatively interact within cattle in Benin and Burkina Faso. This pattern raises new questions regarding the underlying mechanisms and potential consequences.
Risques sanitaires liés à la qualité physico-chimique et bactériologique de l’eau de source souterraine d’Igaga, Fianarantsoa (Madagascar)
Authors: Rabearisoa Andry Harinaina , Harinandrasana Viviane , Rabearisoa Hajandrainy , Rakotozafy Jean Claude Richard , Razanamparany Bruno , Randimbiarison Sedravola Nasolo 
Published: February 2024

L'eau, une ressource vitale, est étudiée pour évaluer les risques sanitaires liés à sa qualité dans la ville de Fianarantsoa, Madagascar. En mars 2023, des analyses ont été réalisées sur des échantillons d'eau prélevés à Igaga, incluant des mesures physico-chimiques telles que la température, le pH, la conductivité électrique, les solides totaux dissous, la turbidité, la demande biologique en oxygène sur cinq jours, le taux d'ion chlorure, ainsi que des analyses bactériologiques couvrant les coliformes totaux et le streptocoque fécaux par la méthode de filtration sur membrane. Les résultats obtenus ont montré que les échantillons prélevés présentaient une valeur moyenne de turbidité 9,19 NTU dépassant la norme établie, ils ont été faiblement minéralisés avec la valeur moyenne de conductivité 130,5 µS/cm et légèrement acide. De plus les eaux ont été contaminées par des germes bactériologiques de coliforme totaux en 38,50 colonies/100 ml en moyenne et streptocoques fécaux en 43 colonies /100 ml en moyenne. En conclusion, l'eau de source d'Igaga présentait des risques potentiels pour les utilisateurs, particulièrement en ce qui concerne les maladies d'origine hydrique. Cette augmentation des germes fécaux pourrait être attribuée au ruissellement après la saison des pluies et au manque d'assainissement et d'hygiène dans l'environnement de la source.

English title: Health risk related to the physic-chemical and bacteriological quality of groundwater Igaga Fianarantsoa (Madagascar)
Water, a vital resource, is being studied to assess health risks associated with its quality in the city of Fianarantsoa, Madagascar. In March 2023, analyses were conducted on water samples collected in Igaga, including physico-chemical measurements such as temperature, pH, electrical conductivity, total dissolved solids, turbidity, five-day biochemical oxygen demand, chloride ion concentration, as well as bacteriological analyses covering total coliforms and fecal streptococci using the membrane filtration method. The results showed that the sampled water had an average turbidity value of 9.19 NTU, exceeding the established standard. They were weakly mineralized with an average conductivity value of 130.5 µS/cm and slightly acidic. Furthermore, the water was contaminated with bacteriological germs, with an average of 38.50 colonies/100 ml for total coliforms and 43 colonies/100 ml for fecal streptococci. In conclusion, Igaga's spring water presented potential risks for users, particularly regarding waterborne diseases. This increase in fecal germs could be attributed to runoff after the rainy season and the lack of sanitation and hygiene in the source environment.
Manual digital de dibujo artístico para el desarrollo de habilidades técnicas en los estudiantes del 4º año de la E.T.A.V. Martín Tovar y Tovar
Authors: Hern´andez José 
Published: February 2024

El presente artículo bajo modalidad de informe de investigación, tiene como objetivo reportar el desarrollo de una investigación donde se propus un manual digital de dibujo artístico para el desarrollo de habilidades técnicas en los estudiantes del 4º año de la E.T.A.V. Martín Tovar y Tovar. El desarrollo de la investigación estuvo basado en el enfoque cuantitativo, paradigma positivista, bajo la modalidad de proyecto especial, apoyado en una investigación de campo con carácter descriptivo. Se desarrolló en tres fases: la primera el diagnóstico a fin de determinar la necesidad de diseñar el manual digital, se tomaron como población el 30% de los estudiantes cursantes de 4to año de la mención arte puro del año escolar 2022-2023. En la segunda fase, se precisaron los elementos necesarios para la elaboración del manual empleando el diseño instruccional modelo ADDIE, y la tercera fase correspondiente a la validación de la propuesta diseñada con expertos. Los resultados obtenidos en este trabajo, permitiron la configuración el recurso digital que servirá como apoyo a los recursos tecnológicos necesarios para tal fin