bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Post‐larval Development of Lateral Musculature in Gilthead Sea Bream Sparus aurata (L.) and Sea Bass Dicentrarchus labrax (L.)
Authors: Ramírez‐Zarzosa G. , Gil F. , Vázquez J. M. , Arencibia A. , Latorre R. , López‐Albors O. , Ortega A. , Moreno F. 
Published: June 2007

SummaryFibre‐type differentiation of lateral musculature has been studied in gilthead sea bream Sparus aurata (L.) and sea bass Dicentrarchus labrax (L.) during post‐larval development using ultrastructural, histochemical and morphometric techniques. The study showed three muscle layers: red, intermediate (or pink) and white. Initially, most of the red muscle showed low myosin ATPase (m‐ATPase) activity fibres, whereas near the transverse septum some small high m‐ATPase activity fibres appeared and later acquired a rosette aspect. Afterwards, during adult growth the red muscle showed a histochemical mosaic appearance. The pink muscle in sea bass was observed at the beginning of juvenile development by the oxidative technique (NADH‐RT reaction) whereas in gilthead sea bream it was also observed at the end of larval development. The pink layer consists of high m‐ATPase activity fibres. However, along the muscle development other low and moderate m‐ATPase activity fibres were observed close to the red and white muscles, respectively. The white muscle of juvenile fish showed a histochemical mosaic appearance near the pink muscle. In adult specimens the mosaic white muscle spread out occupying the whole of the myotome. Morphometric analysis shows a significant increase in mean fibre diameter during post‐larval development, as shown by the Student's t‐test (hypertrophic growth). Skewness and kurtosis values of fibre diameters point to the generation of a new fibres from the myosatellite cells (hyperplastic growth).
Production and Immunohistochemical Characterization of Monoclonal Antibodies Against Ovarian Antigen of the Common Tree Shrew (Tupaia glis)
Authors: Kimura J. , Mukawa H. , Endo H. , Rerkamnuaychoke W. , Kurohmaru M. , Yamada J. , Tsukise A. , Nishida T. 
Published: June 2007

SummaryTo study the ovarian function of the common tree shrew (Tupaia glis), monoclonal antibodies (MAb) against its ovarian antigen were produced. Several positive hybridomas were cloned and MAb‐T2C9 (IgG) was examined for reactivity. Strong immunoreactivity of MAb‐T2C9 was localized within the cytoplasm of luteal cells and in the theca interna cells of Graafian follicles. MAb‐T2C9 reacted also with testicular interstitial cells and adrenocortical cells, except those of the zona glomerulosa. Similar reactions were seen in the goat and sheep. Western blotting analysis of the ovine corpus luteum after reaction with MAb‐T2C9 revealed a single positive band of ≅60kDa. These findings suggest that MAb‐T2C9 recognized protein molecules related to steroid synthesis.
Morphometrical Features of the Corneal Epithelium in Mammals
Authors: Merindano M. D. , Canals M. , Potau J. M. , Costa J. , Ruano D. 
Published: June 2007

SummaryThe study has been conducted on 40 species of mammals included in different taxonomic orders: 10 carnivores (felines and canides), 13 primates and 17 herbivores (artiodactyles, perisodactyles and proboscideans). Domestic, experimental and wild animals have been included in the sample.The purpose of the study is to offer new data regarding morphometric aspects of the epithelium of the cornea of mammals. We also aim to establish the relationships and differences between the different epithelia studied and to determine the morphometric characteristics which best define the epithelium of the different species under study.The results obtained have demonstrated the existence of correlations between thicknesses, number of cellular layers and number of the epithelial cells in the different groups. It has also been established that the variants in thickness and number of layers define and characterize the epithelia. Finally, the study reveals that morphometric characteristics of the epithelium of primates and carnivores have more similarities than those of herbivores.
Histological and Immunohistochemical Study of the Cat Epididymis
Authors: Sánchez B. , Flores J. M. , Pizarro M. , García P. 
Published: June 2007

SummaryA histological and immunohistochemical study of the epididymis of 21 cats which ranged from 7 to 8 months to 2 years of age was made. Five different cell types were observed in the feline epididymis: principal and basal cells, which were the most numerous, apical and narrow, PAS positive cells, which were scarce, and migratory cells, consisting of lymphocytes and macrophages. Four morphologically distinct areas of the cat epididymis were identified. Region I displayed a stellate lumen and the principal cells were tall and presented long and irregular stereocilia. In region II, the lumen was oval and the principal cells were shorter than in the inicial area. Region III displayed characteristics similar to those of region I; the principal difference was the presence of short and regular stereocilia on the surface of the principal cells. In region IV the lumen was very spacious and the epithelium shorter than in the other regions. In regions I, II, and III intraepithelial cavities were observed. With regard to the immunohistochemical results, the basal cells displayed medium immunoreactivity with vimentin. Due to its anti‐desmin reactivity, the muscle wall which surrounds the epididymis was seen to become progresively thicker as it nears the tail. Broad‐spectrum anti‐cytokeratin serum produced intense immunostaining in the basal, apical and narrow, PAS positive cells; the principal cells of region IV also displayed a strong immunoreactivity.
Chemoreceptive Epithelioid Cells in the Chicken Aorta. An Electron Microscopical Study
Authors: Kon Y. , Miyoshi M. , Hashimoto Y. 
Published: June 2007

SummaryAggregations of granulated epithelioid cells exist in the chicken aorta. In the present study, the locational characteristics of the epithelioid cells were investigated via an electron microscopic approach. Epithelioid cells were found mainly in two regions; the tunica interna just below the endothelial cell layers, and the intermediate region of the tunica media. In the former, the epithelioid cells were in contact with endothelial cells and sometimes directly exposed to the aortic lumen. No contact between the epithelioid cells and the nerve endings was observed in this region. On the other hand, in the intermediate region of the tunica media, five or more epithelioid cells were aggregated, and the cynaptic structures were occasionally observed. Additionally, the epithelioid cells in this region were observed to closely contact smooth muscle cells. In an experiment using horseradish peroxidase injection, reactions were detected in the intercellular spaces between the epithelioid cells and endothelial cells. These results suggest that the epithelioid cells localized in the subendothelial region receive information from the aortic lumen, whereas their aggregations in the tunica media are controlled by the nervous system.
Morphological and Immunohistochemical Considerations on the Basal Ganglia in Pigeon (Columba livia)
Authors: Galatioto S. , Abbate F. , Laura R. , Naccari F. , Germana G. 
Published: June 2007

SummaryMorphological and immunohistochemical studies carried out particularly around the level of the basal ganglia (BG) in birds, are reported and commented on.Our results showed, on paraffin embedded avian BG, both the histological features and the immunohistochemical findings on immunofluorescence distribution of some neuropeptides (especially Metenkephalin) in the striatal complex. By comparing our results of Metenkephalin immunoreactivity (Menkir) with the referred analogous ones of Substance P (SP) quoted in literature, we confirmed the strikingly similar labelling at the levels of the Lobus paraolfactorius (LPO) and Paleostriatum augmentatum (PA), in contrast with the very low immunoreactivity at the Paleostriatum primitivum (PP) levels. We were able also to demonstrate the strong MEnk‐ir of the neurons of the Nucleus accumbens, Nucleus septalis and paraventricularis. All these findings are also in accord to the interpretation by many AA (Wynne and Gunturkun. 1995; Reinez et al., 1983). about the anatomical correspondence between the LPO‐PA complex of birds and the caudate‐putamen in mammals. Some MEnk +‘wooly like fibres’ described in our specimens in the PA (on the contrary reported in the Gpe segment of mammals) apparently escape these correspondence.
An Immunohistochemical Study of Bovine Palatine and Pharyngeal Tonsils at 21, 60 and 300 Days of Age
Authors: Manesse M. , Delverdier M. , Abella‐Bourges N. , Sautet J. , Cabanié P. , Schelcher F. 
Published: June 2007

SummaryAn immunohistochemical study was performed on three groups of young cattle (21, 60 and 300 days of age). Tonsils (palatine and pharyngeal) and mucosae (nasal and oral) were removed. Eight monoclonal antibodies (specific for CD3, CD2, CD4, CD8, WC1, cell‐surface IgM, cell‐surface IgG and MHC class II molecules) and an avidin/biotin complex method on frozen sections were used.The immunological cytoarchitecture of bovine tonsils is similar to that of human tonsils. Nevertheless, these lymphoid tissues are not fully developed during the first weeks of life: T and B dependent areas not well‐differentiated, few germinal centres, few intra‐epithelial WC1 + T lymphocytes. In contrast, at 2 months, tonsils possess all the elements of a mucosa‐associated lymphoid tissue (MALT).Tonsillar or mucosal epithelium is infiltrated by a large number of CD8+, WC1+ T lymphocytes and cells which express MHC class II molecules. Between 21 and 60 days, the number of WC1+ T lymphocytes increase markedly in the tonsillar epithelium. These results accredit the hypothesis that the presence of antigens has an effect on the localization of these lymphocytes at these sites.
Distention of the Lateral Intercellular Spaces (LIS) in the Proximal Tubule Cells of the Non‐Stenosed Kidney of the 2K‐1C Goldblatt Model of Hypertension as Evidence of Pressure Diuresis
Authors: Al‐Qattan K. K. , Safer A. M. , Al‐Hajri D. K. 
Published: June 2007

SummaryThis study shows the development of two major deformities in the non‐stenosed kidney of the 2K‐1C Goldblatt model; namely the widening of the LIS and the enlargement of the basilar interdigitations of the proximal tubule cells. These deformities were much less in the 2K‐1C animals treated with the angiotensin I converting enzyme inhibitor (AICEI) cilazapril. From these findings it is suggested that the non‐stenosed kidney is operating under the diuretic effect of the elevated systemic blood pressure (SBP) via an increase in the renal interstitial hydrostatic pressure (RIHP). Therefore, the AII antidiuretic effect is masked by the diuretic effect of the elevated SBP. The suggested rise in urine output fits well with the idea that kidneys lose water and sodium when SBP increases enormously. Therefore, in this model of hypertension, the non‐stenosed kidney tries to lower SBP by losing water and sodium, an excretion behavior which is opposite to that of the stenosed kidney. Thus, the rise in SBP in this model is probably due to an increase in the vascular peripheral resistance rather than fluid accumulation.
Detection of the Ki‐67 Proliferation Associated Nuclear Epitope in Normal Canine Tissues Using the Monoclonal Antibody MIB‐1
Authors: Laprie C. , Abadie J. , Amardeilh M. F. , Raymond I. , Delverdier M. 
Published: June 2007

SummaryThis report describes the immunohistochemical detection of the Ki‐67 proliferation associated nuclear epitope by means of the MIB‐1 monoclonal antibody (mAb) in paraffin tissue sections from nine samples of 16 tissues obtained from nine dogs. Three parameters were considered: the localization of the cells expressing the Ki‐67 epitope, the cytological characteristics of the Ki‐67 expression, and the proliferative activity of some of the tissues measured by means of the proliferative index (percentage of Ki‐67 positive cells measured on 500 and 1000 cells). The MIB‐1 mAb reacts with the nuclei of proliferating cells, as in humans. The proliferative index was far from representative, but we were able to give a range of values concerning the proliferative activity of normal tissues. This study serves as a basis for the expression of the Ki‐67 antigen by normal canine tissues in order to visualize the proliferative compartments and, thus, allows its application as a proliferative marker in routine veterinarian histopathology.
Untersuchungen zur Form der Schilddrüse (Glandula thyreoidea) beim Geflügel mit besonderer Berücksichtigung der jahreszeitlichen Schwankungen
Authors: Breit S. , König H. E. , Stöger E. 
Published: June 2007

SummaryThe thyroid glands of 31 chickens at the age of 17 to 24 months were investigated. Different methods of anatomical preparation, casts of vessels and scanning electron microscopy were used. The thyroid gland of birds is a paired organ. It is located on the ventral surface of the base of the neck within the thoracic inlet. The left thyroid gland is placed more cranially than the right one. Each thyroid gland is closely connected to the common carotid artery on the medial side, from which it is supplied and to the jugular vein on the lateral side. It is a reddish‐brown organ and of lenticular profile. The gland measures on average 10 mm in length, 6 mm in width and 2 mm in thickness, and is covered by a thin connective tissue capsule which holds adipose cells. It seems that each thyroid follicle is surrounded by a net of capillaries. The investigation by scanning electron microscopy proved that the follicles are oval with a pyramidal top on each end. The cuboidal epithelium cells leave impressions in the colloid. Epithelium cells carry microvilli on the follicle side surface. Described seasonal changes of the thyroid gland in size and activity were able to be confirmed by the examination of the organ in July and December. In winter the follicular cells were higher and the follicles had a greater volume.
Histomorphometric and Progesterone Receptor Immunohistochemical Analysis in the Oviduct of Newly Hatched Chicks Treated with Follicle‐Stimulating Hormone during Embryonic Development
Authors: González‐Morán G. , Camacho‐Arroyo I. 
Published: June 2007

SummaryIn this study we evaluated the histomorphology and ultra‐structure of the oviduct of newly hatched chicks, as well as the immunohistochemical expression of progesterone receptor (PR) in this tissue after follicle‐stimulating hormone (FSH) treatment on days 13, 15 and 17 of embryonic development.Results indicated a marked difference in the histology of the oviduct of newly hatched chicks treated with FSH. Magnum mucosa from these animals presented a pseudostratified epithelium with evaginations from the lumen into the epithelium and from the latter into the stroma beneath where tubular glands are formed. In contrast magnum mucosa from control animals presented columnar epithelium with no evaginations. In magnum epithelium FSH also induced the formation of cilia and microvilli projections into the lumen as well as an increase in the wall and lumen areas and in the density of nuclei per unit‐area. PR immunoreactivity was only observed in the oviduct of FSH treated animals. PR was located in the nucleus of epithelial luminal cells, mucosal stromal cells and smooth muscle cells. These findings suggest that FSH induces an adequate hormonal milieu for the cytodifferentiation and PR gene expression in the chick oviduct.
3D Reconstruction of the Mouse's Mesonephros
Authors: Vazquez M.‐D. , Bouchet P. , Mallet J.‐L. , Foliguet B. , Gérard H. , LeHeup B. 
Published: June 2007

SummaryThe present work reports on the three‐dimensional reconstruction of the segmented mesonephros during the embryonic development of the mouse. With a light microscope and an automatic reconstruction of surfaces, aspects of the mesonephros are described. These surfaces are obtained by using digitized contour lines. A new interpolation method called DSI (Discrete Smooth Interpolation) enables correction of the distortion induced by microtomy in paraffin sections. After a tri‐angulation step, this method uses a smoothing algorithm, which implies a spatial redistribution of the vertices of the triangles to correct the rotational and translational misalignment. The use of this 3D program improves the understanding of the development patterns and helps us to appreciate changes in the rebuilt mesonephros. By 10.5 embryonic days, tubules emerge from the Wolffian body and begin their formation, then between 11.5 and 13.5 embryonic days, tortuous mesonephric tubules bound to the Wolffian duct form small curls, which grow and finally unwind. At the same time, mesonephric tubules unbound to the Wolffian duct appear, and on 13.5 embryonic days, the Müllerian duct is visible. After 14.5 embryonic days, the segmented mesonephros keeps its general aspect but decreases in size. At this time, each gonad is provided with both Wolffian and Müllerian ducts. Later, the Wolffian duct differentiates into the definitive male duct system, whilst the Müllerian duct regresses. Conversely, the paramesonephric duct differentiates into the definitive female duct system, whilst the mesonephric duct in turn degenerates. By this time degeneration has begun in the cranial portion of the mesonephros and this process progresses caudally. The spatial organization of the mesonephric tubules and the precise organization of all connections between these elements and the ducts may be well defined. Such approach can allow for a high definition of the normal pattern of mesonephros differentiation.
Morphology and Development of the Distal Ganglion of the Vagus Nerve (Ganglion distale n. vagi) in Sheep during the Prenatal Period
Authors: Pospieszny N. , Brużewicz S. 
Published: June 2007

SummaryThis study was carried out on 31 sheep fetuses, which were divided into four developmental groups. It was established that the development and morphology of the investigated part of vagus nerve is strongly correlated with the developing organs of the fetuses. The very distinct ‘translocation’ of the distal ganglion of the vagus nerve in the caudoventral direction was observed in the course of development. There was no influence of sex on the results of investigation.
The Comparative Anatomical Study of the Parietal Region of the Skull of the Korean Native Goat (Capra Hircus)
Authors: Yi S. J. , Lee H. S. , Kim K. S. , Kang T. C. 
Published: June 2007

SummaryIn the skull of the Korean native goat, the parietal region was classified into four types by the degree of the fusion of the bones, the os interparietale, the os parietale and the squama occipitalis of the os occipitale, and the structural variations of these fusions. The fusion appeared first in the sutura inter‐parietoparietalis and that of the sutura sagittalis of both ossa parietalia was followed. There was no fusion between the os parietale and the squama occipitalis of the os occipitale. These results suggest that the os interparietale developed independently but fused to the os parietale after birth, and the os parietale were developed as paired bones in prenatal life and then fused together according to age.
The Guttural Pouch is not Present in the White Rhinoceros (Ceratotherium simum); Morphology of the Eustachian Tube and Nasopharynx
Authors: Endo H. , Manglai  , Fujisawa M. , Kurohmaru M. , Hayashi Y. 
Published: June 2007

SummaryThe nasopharynx and eustachian tube (auditory tube) were morphologically examined in the white rhinoceros. The narrow nasopharynx cavity was enlarged dorsoventrally. The most lateral part of both sides sharply rises in a dorsal direction and the volume is estimated at 173 cc. The eustachian tube is 145 mm in length from the auditory bulla to the nasopharynx. The hyaline cartilage is well‐developed in the middle region of the eustachian tube wall. The results demonstrated that the white rhinoceros does not have a guttural pouch. We suggest that the occurrence of the guttural pouch may not be dependent on the phylogenetic status of perissodactyls such as horse, donkey and tapir.
Über die frühfetale Entwicklung der Dünndarm‐Mukosa des Rindes (Bos primigenius taurus).
Authors: Winkler Fr. , Wille K.‐H. 
Published: June 2007

SummaryThe development of the bovine small intestine was examined in 24 embryos and fetuses by light microscopic, scanning and transmission electron microscopic methods. Special reference was paid to the genesis of the epithelium and particularly of the villi intestinales.The primitive intestine consists of one layer of epithelial cells surrounded by mesenchym and tunica serosa. The fetal intestine (up to the 24th week of gestation) shows all the morphologic structures of the adult. In small intestine the development of cryptae and villi intestinales starts before the 7th week of gestation and progresses with a proximo‐distal gradient. Epithelial proliferation that gives rise to primary epithelial villi makes epithelium become temporarily stratified. Finger‐like secondary villi develop by proliferation of the mesenchym. In addition to this process mucosal folds occur in duodenum giving rise to villi by segmentation. At the same time the differentiation of epithelium starts.The fetal small intestine, like many other fetal tissues displays masses of glycogen.
The Treatment of Ichthyosis and Hyperkeratotic Conditions with Urea
Authors: Rosten Morris 
Published: June 2007

SUMMARYFifty‐eight patients suffering from a variety of dermatoses were treated with 10% urea in a non‐greasy base. Excellent results were obtained in patients with ichthyosis and other hyperkeratotic conditions. In atopic dermatitis, disseminated neurodermatitis, hand dermatitis and seborrhœic dermatitis the results were only fair. No improvement was noted in psoriasis, pustular psoriasis, solar keratosis and peri‐oral dermatitis.
MELANOMA SPECIFIC PROTEIN: DETECTION AND CHARACTERIZATION OF A TUMOUR SPECIFIC PROTEIN FROM MELANOTIC URINE*
Authors: Bennett Claire , Cooke K. Brian 
Published: June 2007

SummaryUrine from patients with malignant melanoma contains a specific protein not detected in normal urine nor in the urine of any other cancer patients tested. This protein is also found in urine from patients with actively developing halo naevi. It is detected by its ability to inhibit the cytoplasmic staining of malignant melanoma cells by rabbit antimelanoma antisera. The protein is sialo‐protein, molecular weight 76,000 daltons, with α1 globulin mobility. It is suggested that it may be related to the melanosomal proteins.
A DOUBLE‐BLIND CONTROLLED EVALUATION OF DIPROSONE CREAM 0.05% IN COMPARISON WITH SYNALAR CREAM 0.01% IN THE TREATMENT OF PSORIASIS AND OTHER STEROID RESPONSIVE DERMATOSES
Authors: Lewis M. B. 
Published: June 2007

SummaryA three‐week double‐blind randomized study was carried out to compare the effect of Diprosone cream 0.05% with Synalar cream 0.01%. Forty patients suffering from psoriasis and other steroid responsive dermatoses were included in the study. The disease status was carefully monitored throughout the period of the study and parameters were measured to determine the efficacy, onset of action, tolerance and cosmetic acceptability of the two preparations under study. It was concluded that both preparations were efficacious in the treatment of psoriasis and other steroid responsive dermatoses, and that there was no statistically significant difference between the two preparations in the parameters evaluated, other than in the treatment of psoriasis when the parameter of scaling was measured.Side effects were recorded in two patients receiving Synalar, and one receiving Diprosone, during this study.