bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ground Water Flow Parameterization of an Appalachian Coal Mine Complex
Authors: Winters William R. , Capo Rosemary C. 
Published: December 2005

AbstractWe examined a large (240 km2) northern Appalachian bituminous coal basin (Irwin Syncline, Westmoreland County, Pennsylvania) comprising 27 mine complexes with nine major (> 2.5 × 103 L/min) discharges. The synclinal basin was divided into seven subbasins based on equilibrium hydraulic relationships established during the past 25 years. Recharge rates, mine pool velocity, and residence times respond to hydraulic changes in the overburden induced by mine subsidence. The estimated maximum depth for subsidence fractures is 60 m (30 times mined thickness) with recharge rates decreasing significantly in subbasins with thicker overburden (> 75 m). Calculated subbasin recharge rates range from 2 to 6 × 10−4 L/min/m2 and are significantly lower than the previously used rate for the basin. Residence time of ground water in the Irwin subbasins calculated using average linear velocity ranged from one to five years and were more consistent with field observations than estimates obtained using discharge and basin volume area. A positive correlation (r2= 0.80) exists between net alkalinity of the mine water‐impacted discharges and residence time in the mine pools. Our results for the Irwin coal basin suggest that use of a subbasin approach incorporating overburden depth, mining methodology, and the extent of postmining inundation will lead to improved determination of ground water flow parameters in mined watersheds in northern Appalachia and elsewhere.
Scale Effect and Calibration of Contaminant Transport Models
Authors: Wang Fuli , Bright John 
Published: December 2005

AbstractThe influence on solute transport of the small‐scale spatial variation of aquifer hydraulic conductivity (K) was analyzed by comparing results from fine‐grid (2 m by 2 m) simulations of a synthetic heterogeneous aquifer to those from coarse‐grid (8 m by 4 m) simulations of an equivalent homogeneous aquifer. Realizations of the K field of the heterogeneous aquifer were generated, using the Monte Carlo approach, from a lognormal distribution with mean log K of 2 (K in m/d) and three levels of log K variance of 0.1, 0.5, and 1.0. Numerical simulation results show that the average standard deviation of point concentrations increased from 1.21 to 5.78 when the value of log K variance was increased from 0.1 to 1.0. The average discrepancy between modeled concentrations (obtained from a coarse‐grid deterministic numerical simulation) and the actual mean point concentrations (obtained from fine‐grid Monte Carlo numerical simulations) increased from 0.91 to 4.23 with the increase in log K variance. The results from this study illustrate the uncertainty in predictions from contaminant transport models due to their inability to simulate the effects of heterogeneities at scales smaller than the model grid.
Transport of Pharmaceutically Active Compounds in Saturated Laboratory Columns
Authors: Scheytt Traugott , Mersmann Petra , Leidig Marcus , Pekdeger Asaf , Heberer Thomas 
Published: December 2005

AbstractOccurrences of pharmaceutically active compounds in surface water and sewage water have been widely reported. Investigations show the presence of several classes of pharmaceuticals such as antirheumatics (e.g., diclofenac), analgesics (e.g., propyphenazone), and blood lipid regulators (clofibric acid), even in ground water. Compared to their occurrences in surface water, however, the reported incidences of drugs in ground water are much rarer. This may be due to the input, but also to transport processes and degradation in the aquifer.In field studies investigating ground water sampled at a bank infiltration site at Lake Tegel, Berlin, Germany, clofibric acid was found at concentrations up to 290 ng/L, and propyphenazone up to 250 ng/L, whereas concentrations of diclofenac were around the detection limit.The aim of this study was to investigate the ground water transport behavior of the pharmaceuticals clofibric acid, propyphenazone, and diclofenac with a laboratory soil column experiment. Results show that clofibric acid exhibits no degradation and almost no retardation (Rf= 1.1). Diclofenac (Rf= 2.0) and propyphenazone (Rf= 1.6) are retarded, whereas significant degradation was not observed for both pharmaceuticals under the prevailing conditions in the soil column. We conclude that the concentration distribution of the pharmaceuticals at the bank filtration site at Lake Tegel is controlled by sorption, desorption, and input variation, rather than by degradation.
A Tracer Dilution Method for Fracture Characterization in Bedrock Wells
Authors: Brainerd Richard J. , Robbins Gary A. 
Published: December 2005

AbstractThis investigation was undertaken to develop an integrated method of downhole fracture characterization using a tracer. The method presented can be used to locate water‐bearing fractures that intersect the well, to determine the ambient fracture flow rate and hydraulic head, and to calculate fracture transmissivity. The method was tested in two fractured crystalline bedrock wells located at the University of Connecticut in Storrs. The method entails injecting a tracer (uranine dye) into the well, while at the same time water is pumped out of the well. After steady‐state conditions are reached, a borehole tracer concentration profile is developed. The dilution of the tracer is used to locate the inflowing fractures and to determine their flow rate. The fracture flow rate, plus the drawdown in the well, is then used to determine the fracture hydraulic head, transmissivity, and ambient flow rate.
Regulation of neuronal type genes in congestive heart failure rats
Authors: Andersson K. B. , Florholmen G. , Winer L. H. , Tønnessen T. , Christensen G. 
Published: December 2005

AbstractAim:  After myocardial infarction (MI), complex changes in the heart occur during progression into congestive heart failure (CHF). This study sought to identify regulated genes that could have a functional role in some of the changes seen in CHF.Methods:  Myocardial infarction was induced by ligation of the left anterior descending coronary artery (LAD) in Wistar rats. Gene expression changes in 1‐ and 7‐day MI left ventricular myocardium was analysed using complementary DNA (cDNA) filter arrays. Regulated genes were identified by repeated measurements and a ranked ratio analysis method.Results:  A total of 135 genes were identified as differentially expressed. A few genes were robustly regulated at 1‐day MI. In 7‐day CHF hearts, changes in the expression of neuronal type genes was prominent (32%, n = 28). Eleven of these genes with no described association with CHF were selected for validation. One gene failed the validation. In CHF hearts, the expression of the muscarinic m4 (Chrm4) and nicotinic α4 (Chrna4) acetylcholin receptors, the ATP receptor P2rx4, nerve growth factor receptor (Ngfr), discoidin domain receptor 1 (Ddr1), neuronal pentraxin receptor (Nptxr), peripheral myelin protein Pmp‐22, leukocyte type 12‐lipoxygenase (Alox15), cytochrome P450 4F5 (Cyp4F5) and cardiac Kcne1 were all increased (range 1.6–6.0‐fold, P < 0.01 for all genes). The lack of significant regulation of these genes at 1‐day post‐MI, suggests that the induction of these genes at 7‐day post‐MI is not a short‐term response induced by the infarct itself.Conclusion:  These neuronal type genes may participate in underlying processes that affect contractility, intracardiac nerve function and development of arrhythmias in CHF hearts.
ECL cell histamine mobilization and parietal cell stimulation in the rat stomach studied by microdialysis and electron microscopy
Authors: Fykse V. , Solligård E. , Bendheim M. Ø. , Chen D. , Grønbech J. E. , Sandvik A. K. , Waldum H. L. 
Published: December 2005

AbstractAim:  Gastrin stimulates acid secretion by mobilizing histamine from enterochromaffin‐like (ECL) cells that occur predominantly at the base of the gastric glands. The parietal cells occur higher up in the glands nearer to the gastric lumen. The present study was performed to assess whether histamine is transported from the ECL cell via the microcirculation (endocrine route) or local diffusion (paracrine route).Methods:  Totally isolated, vascularly perfused, rat stomachs were examined both in basal and gastrin‐stimulated state. Histamine concentrations, determined by radioimmunoassay in venous effluent and microdialysate from an indwelling probe in the submucosa, were monitored over a period of 240 min. Gastrin‐17 was infused through an arterial catheter for 120 min. The parietal cells were examined by electron microscopy, and the percentage of actively secreting parietal cells (displaying secretory canaliculi) in four regions along the glands (basal to surface, zones I–IV) was determined.Results:  Gastrin stimulated acid secretion and histamine release as well as parietal cell activation. Upon gastrin stimulation, histamine concentration in the microdialysate was 2.5‐fold higher than in the venous effluent (P = 0.008). The parietal cells in the upper part of the gland (zone III) were found to be activated the most.Conclusion:  As the histamine concentrations were higher in the tissue (microdialysate) than in blood, histamine seems to reach the parietal cells via the paracrine route. The fraction of active parietal cells seems to depend more on the age of the parietal cells than on the distance from the ECL cell.
Fluctuations in carotid arterial distensibility during the menstrual cycle do not influence cardiovagal baroreflex sensitivity
Authors: Hayashi K. , Miyachi M. , Seno N. , Takahashi K. , Yamazaki K. , Sugawara J. , Yokoi T. , Onodera S. , Mesaki N. 
Published: December 2005

AbstractAim:  Fluctuations in autonomic nervous functions throughout the menstrual cycle and the underlying mechanism concerning them are not well known. This study was designed to test the hypothesis that fluctuations in cardiovagal baroreflex sensitivity (BRS) throughout the menstrual cycles of young women are due to fluctuations in carotid arterial distensibility.Methods:  In eight eumenorrhoeic healthy young women (18–24 years), we determined the variations in the carotid arterial distensibility coefficient (DC; via simultaneous ultrasonography and applanation tonometry), cardiovagal BRS (phase IV of the Valsalva manoeuvre and the sequence method; up‐ or down‐sequence spontaneous BRS), and serum oestradiol and progesterone concentrations at five points in the menstrual cycle (menstrual = M, follicular = F, ovulatory = O, early luteal = EL, and late luteal = LL).Results:  Serum oestradiol and progesterone levels were consistent with the predicted cycle phases. Carotid arterial DC fluctuated cyclically, increasing significantly from the M (52.4 ± 4.9 × 10−3 kPa−1, mean ± SE) and F (52.7 ± 4.4) phases to the O (57.6 ± 4.4) phase and declining sharply in the EL (46.0 ± 4.0) and LL (45.1 ± 3.0) phases (F = 6.37, P < 0.05). Contrary to our prediction, however, cardiovagal BRS by the Valsalva manoeuvre (P = 0.73) or sequence method (up‐sequence spontaneous BRS; P = 0.84: down‐sequence spontaneous BRS; P = 0.67) did not change significantly during the menstrual cycle.Conclusion:  The results suggest that, although carotid arterial distensibility fluctuates with the changes in ovarian hormone levels that occur during the menstrual cycle, the fluctuations in carotid arterial distensibility do not influence cardiovagal BRS.
Informing research participants of research results: analysis of Canadian university based research ethics board policies
Authors: MacNeil S D , Fernandez C V 
Published: December 2005

Background:Despite potential benefits of the return of research results to research participants, the TriCouncil Policy Statement (TCPS), which reflects Canadian regulatory ethical requirements, does not require this. The policies of Canadian research ethics boards (REBs) are unknown.Objectives:To examine the policies of Canadian university based REBs regarding returning results to research participants, and to ascertain if the presence/absence of a policy may be influenced by REB member composition.Design:Email survey of the coordinators of Canadian university based REBs to determine the presence/absence of a policy on return of research results to research participants both during an ongoing study and at conclusion. REB coordinators were asked to return a copy of the policy or guidelines and to describe the member composition of their REB.Findings:Of 50 REBs that were contacted 34 (68%) responded and 22 (64.7%) met the inclusion criteria. Two (9.1%) had a policy that governed the return of research results while on a study, and seven (31.8%) following the completion of a study. Presence of an ethicist or a lawyer on the REB did not influence the presence/absence of such policies. No REBs had specific guidelines describing how participants should be informed of results.Conclusions:Most REBs did not require researchers to disclose study results to research participants either during or following a study. Thus this study identifies an ethical shortcoming in the conduct of human research in Canada. It has also demonstrated that there are no clear recommendations by REBs to facilitate the return of results to participants following research projects.
What should we say?
Authors: Savulescu J , Foddy B , Rogers J 
Published: December 2005

Abstract ethics mostly focuses on what we do. One form of action is a speech act. What we say can have profound effects. We can and should choose our words and how we speak wisely. When someone close to us suffers an injury or serious illness, a duty of beneficence requires that we support that person through beneficial words or actions. Though our intentions are most often benign, by what we say we often make the unfortunate person feel worse. Beginning with two personal accounts, this article explains what can go wrong in the compassionate speech of wellwishers, and uncovers some of the reasons why people say things that are hurtful or harmful. Despite a large body of clinical evidence, there is no perfect strategy for comforting a friend or relative who is ill, and sometimes even the best thing to say can still be perceived as insensitive and hurtful. In some cases, we may have good reason to knowingly say a hurtful or insensitive thing. Saying these ‘wrong’ things can sometimes be the best way to help a person in the long term. To complicate matters, there can be moral reasons for overriding what is good for the patient. What kind of admonishments should we make to a badly behaved patient? What is the value of authenticity in our communication with the people we love? These questions demand an ethical defence of those speech acts which are painful to hear but which need to be said, and of those which go wrong despite the best efforts of the wellwisher. We offer an ethical account, identifying permissible and impermissible justifications for the things we say to a person with a serious injury or illness.
R.F. SPUTTERING DEPOSITION OF BUFFER LAYERS FOR Si/YBCO INTEGRATED MICROELECTRONICS
Authors: ROMBOLÀ G. , BALLARINI V. , CHIODONI A. , GOZZELINO L. , MEZZETTI E. , MINETTI B. , PIRRI C. F. , TRESSO E. , CAMERLINGO C. 
Published: December 2005

The aim of the present work is the optimization of the Si /buffer-layer/YBCO multilayer deposition process so as to grow superconducting films of quality suitable for device applications. The structural properties of the Si/CeO 2 system, obtained by RF magnetron sputtering of CeO 2 targets in Ar atmosphere, have been studied. More than 50 films have been deposited and some of them submitted to post-deposition annealing treatments both in N 2 and O 2 atmospheres. The presence of an unwanted amorphous SiO 2 layer at the Si/CeO 2 interface compromises the YBCO c-axis orientation, and therefore the sharpness of the R versus T transition. A newly designed deposition system has been realized: it has been specially conceived for obtaining bi- and tri-layers, adopting two targets in YSZ and CeO 2, respectively. Results on YSZ/Si and CeO 2/ YSZ/Si systems obtained with the new machine are presented and discussed: (100) oriented YSZ films with nominal thickness of 40 nm have been obtained. The CeO 2 film subsequently deposited has the desired (100) orientation. The YBCO film, in the final YBCO/YSZ/CeO 2/ Si configuration, is c-axis oriented.
Concomitant optic nerve transection and chorioretinitis sclopetaria
Authors: Mohammadpour Mehrdad , Soheilian Masoud 
Published: December 2005

Abstract

Background
Optic nerve transection and chorioretinitis sclopetaria may occur following blunt ocular trauma. However, simultaneous occurrence has not yet been reported. We report the first case of concomitant optic nerve transection and chorioretinitis sclopetaria.

Case presentation
A 12- year- old boy with history of BB gun injury to his right eye was referred for loss of vision. His visual acuity was counting fingers at one meter in the right eye and with 3+ relative afferent pupillary defect (RAPD).
On slit lamp examination, the right eye appeared normal except for 1+ vitreous reaction. Fundus examination of the right eye revealed a pale disc with superior retinal scar and diffuse submacular fibrosis compatible with chorioretinitis sclopetaria. Orbital CT- scans showed transection of the optic nerve by the BB gun pellet, which was lodged at the orbital apex.

Conclusion
BB gun injury may cause concomitant optic nerve transection and chorioretinitissclopetaria.
Targeting the active site of the placental isozyme of alkaline phosphatase by phage-displayed scFv antibodies selected by a specific uncompetitive inhibitor
Authors: Saini Deepti , Kala Mrinalini , Jain Vishal , Sinha Subrata 
Published: December 2005

Abstract

Background
The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties and a high degree of identity. The placental isozyme (PLAP) is an oncofetal antigen expressed in several malignancies including choriocarcinoma, seminoma and ovarian carcinoma. We had earlier attempted to isolate PLAP-specific scFv from a synthetic human immunoglobulin library but were unable to do so, presumably because of the similarity between the isozymes.
In this work, we have employed a PLAP-specific uncompetitive inhibitor, L-Phe-Gly-Gly, to select isozyme specific scFvs. An uncompetitive inhibitor binds to the enzyme in the presence of substrate and stabilizes the enzyme-substrate complex. Several uncompetitive inhibitors have varying degrees of isozyme specificity for human alkaline phosphatase isozymes. A specific uncompetitive inhibitor would be able to unmask conformational differences between the otherwise very similar molecules. Also, such inhibitors would be directed to regions at/close to the active site of the enzyme. In this work, the library was first incubated with PLAP and the bound clones then eluted by incubation with L-Phe-Gly-Gly along with the substrate, para-nitro phenyl phosphate (pNPP). The scFvs were then studied with regard to the biochemical modulation of their binding, isozyme specificity and effect on enzyme activity.

Results
Of 13 clones studied initially, the binding of 9 was inhibited by L-Phe-Gly-Gly (with pNPP) and 2 clones were inhibited by pNPP alone. Two clones had absolute and 2 clones had partial specificity to PLAP. Two clones were cross-reactive with only one other isozyme. Three scFv clones, having an accessible His6-tag, were purified and studied for their modulation of enzyme activity. All the three scFvs inhibited PLAP activity with the kinetics of competitive inhibition. Cell ELISA could demonstrate binding of the specific scFvs to the cell surface expressed PLAP.

Conclusion
The results demonstrate the biochemical modulation of scFv binding. Also, the scFvs bound to the active site and denied the access to the substrate. The selection strategy could generate specific anti-enzyme antibodies to PLAP that can potentially be used for targeting, for modulating enzyme activity in in vitro and in vivo and as probes for the active site. This strategy also has a general application in selecting antibodies from combinatorial libraries to closely related molecules and conformations.
Portable light transmission measuring system for preserved corneas
Authors: Ventura Liliane , de Jesus Gabriel Torres , de Oliveira Gunter Camilo Dablas , Sousa Sidney JF 
Published: December 2005

Abstract

Background
The authors have developed a small portable device for the objective measurement of the transparency of corneas stored in preservative medium, for use by eye banks in evaluation prior to transplantation.

Methods
The optical system consists of a white light, lenses, and pinholes that collimate the white light beams and illuminate the cornea in its preservative medium, and an optical filter (400–700 nm) that selects the range of the wavelength of interest. A sensor detects the light that passes through the cornea, and the average corneal transparency is displayed. In order to obtain only the tissue transparency, an electronic circuit was built to detect a baseline input of the preservative medium prior to the measurement of corneal transparency. The operation of the system involves three steps: adjusting the "0 %" transmittance of the instrument, determining the "100 %" transmittance of the system, and finally measuring the transparency of the preserved cornea inside the storage medium.

Results
Fifty selected corneas were evaluated. Each cornea was submitted to three evaluation methods: subjective classification of transparency through a slit lamp, quantification of the transmittance of light using a corneal spectrophotometer previously developed, and measurement of transparency with the portable device.

Conclusion
By comparing the three methods and using the expertise of eye bank trained personnel, a table for quantifying corneal transparency with the new device has been developed. The correlation factor between the corneal spectrophotometer and the new device is 0,99813, leading to a system that is able to standardize transparency measurements of preserved corneas, which is currently done subjectively.