bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic analysis of the tetraspanin web using LC‐ESI‐MS/MS and MALDI‐FTICR‐MS
Authors: André Magali , Le Caer Jean‐Pierre , Greco Céline , Planchon Sébastien , El Nemer Wassim , Boucheix Claude , Rubinstein Eric , Chamot‐Rooke Julia , Le Naour François 
Published: January 2006

AbstractTetraspanins are integral membrane proteins involved in a variety of physiological and pathological processes. In cancer, clinical and experimental studies have reported a link between tetraspanin expression levels and metastasis. Tetraspanins play a role as organizers of a molecular network of interactions, the “tetraspanin web”. Here, we have performed a proteomic characterization of the tetraspanin web using a model of human colon cancer consisting of two cell lines derived from primary tumor and metastasis from the same patient. The tetraspanin complexes were isolated after immunoaffinity purification and the proteins were identified by MS using LC‐ESI‐MS/MS and MALDI‐FTICR. The high resolution and mass accuracy of FTICR MS allowed reliable identification using mass finger printing with only two peptides. Thus, it could be used to resolve the composition of complex peptide mixtures from membrane proteins. Different types of membrane proteins were identified, including adhesion molecules (integrins, Lu/B‐CAM, GA733 proteins), receptors and signaling molecules (BAI2, PKC, G proteins), proteases (ADAM10, TADG15), and membrane fusion proteins (syntaxins) as well as poorly characterized proteins (CDCP1, HEM‐1, CTL1, and CTL2). Some components were differentially detected in the tetraspanin web of the two cell lines. These differences may be relevant for tumor progression and metastasis.
Identification of S‐nitrosylated proteins after chronic exposure of colon epithelial cells to deoxycholate
Authors: Dall'Agnol Monique , Bernstein Carol , Bernstein Harris , Garewal Harinder , Payne Claire M. 
Published: January 2006

AbstractApoptosis resistance, a condition favoring genomic instability, is associated with higher risk of colorectal cancer. Deoxycholate (DOC) is a hydrophobic bile salt found in high concentrations in colon cancer patients, and induces apoptosis in cultured colonic cells and ex vivo in colonic biopsies. We showed previously that the chronic exposure of colon cancer cells to increasing concentrations of DOC leads to apoptosis resistance, and the suggested mechanism involves oxidative/nitrosative stress. Nitric oxide (NO) is a key signaling molecule that regulates cell function in a variety of physiologic and pathophysiologic states. In part, NO exerts its actions by S‐nitrosylation of target thiols, and several proteins are regulated through this PTM, including the caspases, the main effectors of apoptosis. Here, we performed a proteomics study in the DOC‐induced apoptosis‐resistant colon cell line, HCT‐116RC. Its profile of S‐nitrosylated proteins was compared to a control cell line not exposed to DOC. Eighteen differentially S‐nitrosylated proteins were identified in the HCT‐116RC cell line, 14 of these are novel targets of S‐nitrosylation not previously reported. These proteins include cytoskeletal and signaling proteins, metabolic enzymes, chaperones, and redox‐ and differentiation‐related proteins. These results broaden our knowledge of potential signal transduction pathways that may lead to the development of new biomarkers and therapy targets.
Searching for aging‐related proteins in human dermal microvascular endothelial cells treated with anti‐aging agents
Authors: Lee Ju Hee , Chung Kee Yang , Bang Dongsik , Lee Kwang Hoon 
Published: January 2006

AbstractEndothelial cells constitute an interface between blood and tissue and act as a medium for active interaction between plasma and the intracellular environment for homeostasis. Aging of endothelial cells plays a significant role in the pathophysiology of age‐related vascular diseases; however, precise mechanisms for senescence have not been elucidated. Proteomics allows identification of protein structures, functions, and characteristics, and can be applied to the study of aging processes. Using cultured human dermal microvascular endothelial cells and two‐dimensional proteomic mapping, we studied the effects of kinetin, epigallocatechin‐3‐gallate, all‐trans‐retinoic acid, and selenium on their senescence and searched for the aging‐related proteins. The treatments resulted in 68 qualitative changes and 172 quantitative changes, and we were able to identify 46 spots among them. All of the agents indicated above induced changes in the expression of moesin, rho guanosine‐5'‐diphosphate‐dissociation inhibitor, and actin, confirmed by immunoblotting and confocal laser microscopy. As these proteins were associated with cell cycle and cytoskeleton, immunoblotting of the proteins related to cell cycle was performed. Although practical significance remains to be confirmed by in vivo research, this fundamental discovery may provide a basis for understanding the mechanism of aging and age‐related diseases.
Comparative proteomic analysis of Helicobacter pylori strains associated with iron deficiency anemia
Authors: Park Shin Ae , Lee Hyang Woo , Hong Myung Hee , Choi Young Wook , Choe Yon Ho , Ahn Bo Young , Cho Yang Je , Su Kim Dong , Lee Na Gyong 
Published: January 2006

AbstractHelicobacter pylori is known to cause chronic gastritis, peptic ulcer, and gastric cancer, and has also been linked to iron deficiency anemia (IDA). To determine whether H. pylori clinical isolates correlate with the prevalence of H. pylori‐associated IDA, we compared the proteomic profiles of H. pylori strains isolated from antral biopsy specimens of H. pylori‐positive patients with or without IDA. Fifteen strains, including eight non‐IDA and seven IDA strains, were cultured under iron‐rich and iron‐depleted conditions and then analyzed for protein expression profiles by 2‐DE. The distances between two H. pylori strains were determined on the basis of similarities between their expression patterns of 189 protein spots, and a phylogenetic tree was constructed. The results revealed that the IDA strains formed a cluster separate from that of six non‐IDA strains, with two non‐IDA strains between the clusters. H. pylori strain 26695 was located in the non‐IDA cluster. Protein spots displaying similar expression patterns were clustered, and 18 spots predominantly expressed in IDA strains were identified by MALDI‐TOF analysis. These data indicate that the non‐IDA and IDA strains can be distinguished by their protein expression profiles, suggesting that the polymorphism of H. pylori strains may be one of the factors determining the occurrence of H. pylori‐associated IDA.
Mass spectrometric sequencing of endotoxin proteins of Bacillus thuringiensis ssp. konkukian extracted from polyacrylamide gels
Authors: Lee Kwang Yong , Kang Eun Young , Park Sooil , Ahn Seong Kyu , Yoo Kwan Hee , Kim Jin Young , Lee Hyung Hoan 
Published: January 2006

AbstractThe amino acid sequences of the crystal proteins of Bacillus thuringiensis ssp. konkukian strain HL‐47 are unknown. We used 1‐D denaturing polyacrylamide electrophoresis, nano‐ESI‐Q‐TOF‐MS, and protein database searching to analyze these proteins. On SDS‐PAGE gels, a preparation of purified crystal proteins exhibited 110, 102, 76, 55, 37, and 30 kDa protein bands. Immunoblotting of the gel with antiserum raised to this preparation revealed that four crystal proteins, of 110, 102, 55, and 37 kDa, reacted with the specific antiserum. The 102‐kDa major protein reacted strongly. The other crystal proteins showed weak immunoreactivity. The 102 and 55 kDa proteins were analyzed by ESI‐MS. The internal amino acid sequence of the 102‐kDa major protein has similarity to the sequences of the surface layer protein of B. thuringiensis ssp. finitimus and B. anthracis. However, the internal amino acid sequences of the 55 kDa protein did not show any homology to proteins in the databases. Proteomic analysis of these proteins leads to the conclusion that the sequence data provided the protein databases of the crystal proteins of the konkukian ssp.
Profiling of differential protein expression in angiogenin‐induced HUVECs using antibody‐arrayed ProteoChip
Authors: Ahn Eun‐Hee , Kang Dong‐Ku , Chang Soo‐Ik , Kang Chang Soo , Han Moon‐Hi , Kang In‐Cheol 
Published: January 2006

AbstractProteoChip has been developed as a novel protein microarray technology. So far it has been applied in new lead screening and molecular diagnosticsand we expect its role to grow in the field of biology. Here, we investigated the application of ProteoChip for the study of differential protein expression profiles in angiogenin‐induced human umbilical vein endothelial cells (HUVECs). Antibody microarrays constructed by immobilizing 60 distinct antibodies against signal‐transducing proteins on ProteoChip base plates were used to analyze the expression pattern of cell‐signaling proteins in HUVECs treated with angiogenin. The antibody microarray approach showed that angiogenin induced the up‐ and down‐regulation of several cellular regulators related with cell proliferation. Changes in the expression of signaling proteins determined by antibody microarray were validated by Western blot analysis. In this experiment, ten up‐regulated proteins and six down‐regulated proteins were identified and confirmed by immunoblot analysis. Taken together, these data suggest that antibody microarrays using ProteoChip technology can be a powerful tool for high‐throughput analysis of proteomes in biological samples.
Identification of immunodominant excretory–secretory cysteine proteases of adult Paragonimus westermani by proteome analysis
Authors: Lee Eung‐Goo , Na Byoung‐Kuk , Bae Young‐An , Kim Seon‐Hee , Je Eun‐Young , Ju Jeong‐Won , Cho Shin‐Hyeong , Kim Tong‐Soo , Kang Shin‐Yong , Cho Seung‐Yull , Kong Yoon 
Published: January 2006

AbstractParagonimus westermani causes inflammatory lung disease in humans. The parasite excretes a host of biologically active molecules, which are thought to be involved in pathophysiological and immunological events during infection. Analyses of the 2‐DE protein profiles of the excretory–secretory products (ESP) of adult P. westermani revealed approximately 147 protein spots, at least 15 of which were identified as cysteine proteases (CPs), at pHs between 4.5 and 8.5, and molecular weights (MWs) between 27 and 35 kDa. An additional three CPs (designated as PwCP‐3, ‐8 and ‐11) were newly recognized by TOF/TOF MS. Their molecular biological information, which shared a high level sequence homology, was elucidated. The majority of the CPs reacted strongly with sera from paragonimiasis patients. When we observed the chronological changes in the antibody responses of the respective CPs against canine sera collected serially at 1, 3, 5, 7, 11 and 14 wk after experimental infection, these molecules exhibited a multiplicity of distinct immune recognition patterns. Our results clearly showed that P. westermani adult ESP were principally composed of excretory–secretory CPs, and that these CPs may exert effects not only on host tissue degradation and nutrient uptake, but also on the immune‐regulating cells via synergistic and independent interactions.
Identification of target proteins of N‐acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration
Authors: Kim Yong‐Sam , Kang Hye‐Yeon , Kim Jin‐Young , Oh Sejeong , Kim Cheorl‐Ho , Ryu Chun Jeih , Miyoshi Eiji , Taniguchi Naoyuki , Ko Jeong Heon 
Published: January 2006

AbstractTo gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2‐DE and lectin blot, followed by identification based on ESI‐MS. Through these approaches 14 proteins were identified as candidate target proteins for N‐acetylglucosaminyl transferase V (GnT‐V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPκ) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPκ underwent an aberrant glycosylation in GnT‐V‐overexpressing WiDr cells, and the aberrantly glycosylated PTPκ was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPκ in GnT‐V‐overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.
Reactivity of silicon‐based epoxy monomers as studied by near‐infrared spectroscopy and multivariate curve resolution methods
Authors: Mercado L. A. , Galià M. , Reina J. A. , Garrido M. , Larrechi M. S. , Rius F. X. 
Published: January 2006

AbstractThe reaction of glycidyloxydimethylphenyl silane with aniline was used as a model system to study the reactivity of silicon‐based epoxy monomers. The reaction was monitored online by near‐infrared spectroscopy, and the evolution of the concentration of each species throughout the reaction was determined by the application of multivariate curve resolution/alternating least squares to the set of recorded spectra. The reactivity was evaluated by a comparison of the concentration profiles obtained for the glycidyloxydimethylphenyl silane/aniline system with those of phenylglycidyl ether/aniline as a reference system. The results confirmed that the reactivity of the silicon‐based epoxy monomer was higher and that its ring opening reaction was faster because of electronic effects. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 1447–1456, 2006
Effect of temperature on the frictional forces between polystyrene brushes
Authors: Forster Aaron M. , Mays Jimmy W. , Kilbey S. Michael 
Published: January 2006

AbstractNormal and shear forces between opposing polystyrene (PS) brushes made from preferentially assembled PS–polyvinylpyridine diblock copolymers were measured in toluene and in near‐theta cyclohexane at 32, 40, and 50 °C, using a modified surface forces apparatus. In cyclohexane, over the temperature window probed, the normal forces of interaction are repulsive and the range of those force profiles changes only slightly; however, for both of the PS brushes studied, the onset of shear forces in near‐theta cyclohexane is strongly influenced by changes in temperature: As the temperature is increased, the onset of the frictional interactions between the brushes in cyclohexane shifts to smaller distances, approaching the distances where frictional forces are observed for brushes in the good solvent toluene. The pattern of behavior seen in the frictional response between the limits of good and theta condition is attributed to composition fluctuations, which increase near the theta condition because of the decrease in excluded volume interactions. These fluctuations may give rise to increased drag during shearing motion because of interfacial roughness or interchain coupling across the brush–brush interface. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 649–655, 2006
Independence of the brittle–ductile transition from the rubber particle size for impact‐modified poly(vinyl chloride)
Authors: Zhao Chengzhe , Wu Guangfeng , Zhou Chao , Yang Haidong , Zhang Huixuan 
Published: January 2006

AbstractA series of acrylic impact modifiers (AIMs) with different particle sizes ranging from 55.2 to 927.0 nm were synthesized by seeded emulsion polymerization, and the effect of the particle size on the brittle–ductile transition of impact‐modified poly(vinyl chloride) (PVC) was investigated. For each AIM, a series of PVC/AIM blends with compositions of 6, 8, 10, 12, and 15 phr AIM in 100 phr PVC were prepared, and the Izod impact strengths of these blends were tested at 23 °C. For AIMs with particle sizes of 55.2, 59.8, 125.2, 243.2, and 341.1 nm, the blends fractured in the brittle mode when the concentration of AIM was lower than 10 phr, whereas the blends showed ductile fracture when the AIM concentration reached 10 phr. It was concluded that the brittle–ductile transition of the PVC/AIM blends was independent of the particle size in the range of 55.2–341.1 nm. When the particle size was greater than 341.1 nm, however, the brittle–ductile transition shifted to a higher AIM concentration with an increase in the particle size. Furthermore, the critical interparticle distance was found not to be the criterion of the brittle–ductile transition for the PVC/AIM blends. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 696–702, 2006
The effect of angle and oscillation on mucous simulant speed in flexible tubes
Authors: van Vliet Peter W. , Ellis Elizabeth R , Hila Julie 
Published: January 2006

AbstractBackground and Purpose.The aim of this study was to investigate, in a tube model, how the speed of a mucous simulant was influenced by angle and different types of oscillations.Method.Using a repeated‐measures study design, the primary outcome measure was the mucous simulant speed calculated from the time taken for the mucous simulant to travel a distance of 10 cm. Ultrasonic gel diluted to a viscosity (113 Poise), approximating human sputum, was introduced into a flexible tube similar in diameter to the human adult trachea. The tube was subjected to discrete angles of 0° and 30°, 60° and 90° downward. Symmetrical oscillation was applied in both the transverse and longitudinal directions with frequencies of 5, 15 and 25 Hz at amplitudes of 1 mm and 2 mm peak‐to‐peak using a commercially available oscillator. Asymmetrical oscillation was applied using repeated cycles of slow acceleration and fast deceleration in the longitudinal direction at 0° and 30° downward and up a 5° incline using a custom‐built apparatus.Results.Each 30° angle increment of the tube from 0° to 90° significantly increased mucous simulant speed (p<0.001). Symmetrical oscillation did not provide an advantage over angle in terms of mucous simulant speed; however, asymmetrical oscillation increased mucous simulant speed beyond that caused by angle for all angles tested (p<0.001) and was able to drive mucous simulant up a small incline (5°) in this tube model. It was found that certain types of longitudinal oscillation elongated the mucous simulant.Conclusions.The present study supports the use of gravity to assist in secretion clearance. Asymmetrical oscillation is a novel technique which warrants further investigation. Copyright © 2005 John Wiley & Sons, Ltd.
Cardiovascular responses to short‐term head‐down positioning in healthy young and older adults
Authors: Naylor Justine M , Chow Chin‐Moi , McLean Anthony S , Heard Robert C , Avolio Albert 
Published: January 2006

AbstractBackground and Purpose.Isolated head‐down postural drainage is assumed to acutely load the cardiovascular system. Consequently, it is considered a relative contraindication in the presence of severe cardiovascular disease. Evidence demonstrating that the head‐down manoeuvre as used by physiotherapists does significantly load the cardiovascular system is lacking. The present study documents the cardiovascular responses to short‐term 30° head‐down positioning in healthy subjects. The results are a point of reference for respiratory patients with and without cardiovascular disease.Method.A quasi‐experimental research design was used, with multiple measurements obtained at rest (long sitting) and in the head‐down position. Twenty‐one young subjects (mean age 25 years (standard deviation, (SD) 3 years)) and 19 older subjects (mean age 66 years (SD 6 years)) were studied. Applanation tonometry and sphygmocardiography were used to measure temporal and pressure variables, and indices that estimate myocardial work and coronary blood flow.Results.Absolute differences existed between the two age groups for all variables at rest (p< 0.001). No age–time interaction was observed for any variable in the head‐down position (p>0.05). Serial measures in the head‐down position did not vary across time (p>0.05). Small (<9%) but significant (p ≤ 0.02) decreases in heart rate, relative diastolic duration, mean arterial blood pressure and diastolic time indices, and small (<12%) but significant (p ≤ 0.002) increases in cardiac cycle time, ejection duration (relative and absolute) and absolute diastolic duration were observed in the head‐down position compared with rest. A small (9%) but significant (p< 0.001) fall in the sub‐endocardial viability ratio occurred in the head‐down position.Conclusion.The findings have little consequence in health, but they suggest that head‐down postural drainage may be of concern for chest physiotherapy recipients with reduced cardiac reserve or impaired baroreflex function. Copyright © 2005 Whurr Publishers Ltd.
Postural instability during reaching tasks in Parkinson's disease
Authors: Stack Emma , Ashburn Ann , Jupp Kate 
Published: January 2006

AbstractBackground and Purpose.Reaching, like turning and rising from sitting, is an activity commonly associated with falls by people with Parkinson's disease. We set out to: (a) identify how people with moderate and severe Parkinson's disease performed high and low reaches and (b) to explore in detail the standard functional reach (functional reach) test and the fall histories of those who appeared unstable when reaching.Method.In this cross‐sectional study, people with moderate or severe Parkinson's disease (Hoehn and Yahr Grade III or IV) were video‐recorded at home, reaching above shoulder height and below knee level. Blinded observers rated stability, alignment, foot position, floor contact, use of support and reach type. Functional reach was also measured and participants were interviewed about falls.Results.Of 51 participants, 33 (65%) had moderate Parkinson's disease and 18 (35%) severe. A greater proportion of the latter used support when reaching high (p = 0.029) and aligned forward when reaching low (p = 0.015); otherwise, strategies were similar across groups. Six people (all with severe Parkinson's disease) appeared unstable when reaching: they had a shorter functional reach than the others (median 10 cm versus 18 cm; p = 0.042) and had fallen frequently (median five falls in a year), although rarely when reaching.Conclusions.Reaching tasks challenge postural stability in severe Parkinson's disease. People who appear unstable when reaching are likely to be repeat‐fallers and at risk of further falls during more demanding activities. Research should address whether discouraging potentially destabilizing manoeuvres (such as squatting and toe‐standing) and promoting safety‐enhancing strategies (such as using support and facing forward), with or without balance retraining, reduces the risk of falling among people with severe Parkinson's disease. Copyright © 2005 John Wiley & Sons, Ltd.