bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of papnettrade; system for rescreening of negative cervical smears
Authors: Ashfaq R. , Liang Y. , Saboorian M. H. 
Published: February 2007

AbstractWe rescreened 2,238 cervicovaginal smears conventionally prepared with Papanicolaou stain by PAPNETtrade; system. The slides screened manually as negative, were sent to PAPNET system. The image tapes were reviewed on a high‐resolution monitor, and categorized as negative, unsatisfactory, and atypical. All atypical cases were rescreened manually. Abnormal cases were reviewed by a cytopathologist. Two‐thousand one hundred and two (94%) cases rescreened by PAPNET were negative. Nine of 45 unsatisfactory cases by PAPNET were unsatisfactory by manual review. Ninety‐one (4.0%) cases by PAPNET were atypical. On manual rescreening, 86 of 91 were negative, 20% showing benign cellular changes; five of 91 were atypical, the atypia, however, not exceeding low‐grade category. The detection rate by PAPNET method was 0.2% (five of 2,238 cases). We conclude: 1) In a cytology laboratory with good quality control, PAPNET rescreening does not significantly increase the detection rate. 2) For cytology laboratories without in‐house rescreening. PAPNET offers an alternative at a price. 3) The PAPNET system also offers a tool by which a laboratory can occasionally monitor its performance. 4) The cost benefit analysis of the system requires further study and scrutiny. © 1995 Wiley‐Liss, Inc.
Cytomorphologic appearances of normal endometrial cells during different phases of the menstrual cycle: A cytologic approach to endometrial dating
Authors: Tao Liang‐Che 
Published: February 2007

AbstractFrom 1988 to 1992, 156 endometrial cytologic preparations were procured by directly brushing hysterectomy specimens from premenopausal women with normal endometrium and regular menstruation. These brushing smears were obtained by using the cyto‐brush and/or the endometrial brush. The cytologic findings were correlated with histologic endometrial dating. Of these, 56 cases were classified as proliferative phase endometrium; 89, secretory phase; and 11, menstrual phase. The cytomorphologic features of endometrial glandular and stromal cells at different stages of the menstrual cycle are summarized and compared. On the basis of the different cytomorphologic features of glandular and stromal cells seen during various phases of the cycle, cytologic differentiation between proliferative phase and secretory phase endometria can be readily established. It appears possible to date the endometrium by direct endometrial brushing technique. It is also possible to determine if the glandular and/or stromal cells seen in the endocervical smears procured by the cytobrush are normal endometrial cells from cytobrush extraction, and are consistent with the date of the menstrual cycle, which should be made clear in the cytology report. © 1995 Wiley‐Liss, Inc.
Cytology, flow cytometry, image analysis, and interphase cytogenetics by fluorescence in situ hybridization in the diagnosis of transitional cell carcinoma in bladder washes: A comparative study
Authors: Cajulis Ricardo S. , Haines George K. , Frias‐Hidvegi Denise , McVary Kevin , Bacus James W. 
Published: February 2007

AbstractThe diagnosis of transitional cell carcinoma (TCC) in bladder washes is a diagnostic challenge to cytology. This study assessed the role of flow cytometry (FCM), image analysis (IA), and interphase cytogenetics by fluorescence in situ hybridization (FISH) as adjuncts in the cytodiagnosis of TCC in bladder washes. Forty separate samples of bladder washes were prospectively evaluated by conventional cytology (CY), FCM, IA, and FISH, and the results were compared with the subsequent surgical biopsy specimens which revealed 26 TCC (3 GR I, 6 GR II, 17 GR III) and 14 benign lesions. Using histology as the “gold standard” and following the previously published criteria for detection of TCC by CY, FCM, IA, and FISH, the concordance rates between histology and CY, FCM, IA, and FISH were 75, 74, 89, and 83%, respectively. CY, FCM, IA, FISH, and histology were concordant in 54% of the cases. The sensitivity of CY, FCM, IA, and FISH were 61, 72, 91, and 73%. respectively, while the specificity were 100, 80, 83, and 100%, respectively. The combined sensitivity of all the parameters was 96%. Interestingly, the false positive cases by FCM and IA showed cystitis. We conclude that IA has the highest sensitivity in detecting TCC in bladder washes followed by FISH, FCM, and CY, while CY and FISH have the highest specificity. This study indicates that FCM, IA, and FISH are useful adjuncts to cytology in the diagnosis of TCC in bladder washes. The finding of DNA‐aneuploidy in cystitis warrants further investigation. © 1995 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of superficial angiomyxoma (myxoid perifollicular fibroma): Report of a case
Authors: Izquierdo Francisco M. , Martin Luis , Burgos Fernando , Lacruz César 
Published: February 2007

AbstractA case of superficial angiomyxoma arising in the areola of a 49‐yr‐old woman is presented, including both fine‐needle aspiration cytology (FNAC) and histological study. The aspirate yielded a rich myxoid background with isolated fusiform cells, showing oval, monomorphic nuclei with minimal atypia. Sometimes these cells were arranged in small fragments without any specific pattern. The absence of mitotic figures, atypia, or cutaneous and breast epithelium suggested a benign myxoid stromal neoplasm. The final diagnosis was established on the excised mass by histopathologic study. The report of this entity in FNAC and the differential diagnosis with other myxoid lesions are discussed, and the feasibility of its cytological diagnosis by FNAC, helped by appropriate clinical information. © 1995 Wiley‐Liss, Inc.
Biphasic intraabdominal desmoplastic small round cell tumor: Fine‐needle aspiration cytology findings
Authors: Drut Ricardo 
Published: February 2007

AbstractThe present report describes the case of a 9‐yr‐old boy with an abdominal desmoplastic small round cell tumor (DSRCT) which on fine‐needle aspiration cytology and histology revealed a biphasic pattern, making initial diagnosis difficult. Epithelial‐like clusters of cells and loosely‐arranged poorly‐differentiated cells with scant cytoplasm associated with cells having a larger nucleus and multinucleated larger cells represented the smears' counterpart of epithelial clusters and lobules and sarcomatous‐like tissue recognized in the histologic sections. Multinucleated cells were common in the sarcomatous‐like areas of the tumor. The biphasic pattern was highlighted by immunohistochemistry. Keratin and epithelial membrane antigen stained predominantly or only the epithelial component, while desmin diffusely decorated the sarcomatous areas and the epithelial cells as a paranuclear cytoplasmic dot. Immunosera 013 mainly stained the sarcomatous component. © 1995 Wiley‐Liss, Inc.
Metastatic liposarcoma in the adrenal gland: Report of two cases diagnosed by fine‐needle aspiration
Authors: McCutcheon J. , Irvine A. , Derias N. W. 
Published: February 2007

AbstractFine‐needle aspiration is now well established in the investigation of retroperitoneal masses. Many of these lesions are first detected in the course of radiological surveys of patients with known or suspected malignancy. It can be difficult to diagnose sarcomas on fine‐needle aspiration material as many of these tumour do not show cytological features of malignancy. We describe two cases of metastatic liposarcoma confidently diagnosed on the basis of characteristic cytological features seen in material aspirated from metastatic adrenal tumours. © 1995 Wiley‐Liss, Inc.
Prognostic factors in breast cancer: Use of cytologic preparations
Authors: Masood Shahla 
Published: February 2007

AbstractThere is increasing evidence that certain morphological features and biological markers found in breast tumors may provide prognostic information by predicting the risk of recurrence and metastasis in early breast cancer. This information may also be important in choosing therapeutic options in patients with advanced disease. Prognostic testing is commonly performed on surgically excised lesions. However, there are clinical conditions in which a surgical specimen may not be suitable or available for such analysis. In these circumstances, fine‐needle aspiration biopsy and imprint preparation provide an attractive sample for prognostic testings. This review summarizes the current approach to the use of cytologic preparation for assessment of established and newly recognized prognostic factors. © 1995 Wiley‐Liss, Inc.
Structural components of the nonstriated contractile apparatuses in the Caenorhabditis elegans gonadal myoepithelial sheath and their essential roles for ovulation
Authors: Ono Kanako , Yu Robinson , Ono Shoichiro 
Published: February 2007

AbstractOvulation in the nematode Caenorhabditis elegans is regulated by complex signal transduction pathways and cell–cell interactions. Myoepithelial sheath cells of the proximal ovary are smooth muscle‐like cells that provide contractile forces to push a mature oocyte into the spermatheca for fertilization. Although several genes that regulate sheath contraction have been characterized, basic components of the contractile apparatuses of the myoepithelial sheath have not been extensively studied. We identified major structural proteins of the contractile apparatuses of the myoepithelial sheath and characterized their nonstriated arrangement. Of interest, integrin and perlecan were found only at the dense bodies, whereas they localized to both dense bodies and M‐lines in the striated body wall muscle. RNA interference of most of the myofibrillar components impaired ovulation in a soma‐specific manner. Our results provide basic information that helps understanding the mechanism of sheath contraction during ovulation and establishing a new model to study morphogenesis of nonstriated muscle. Developmental Dynamics 236:1093–1105, 2007. © 2007 Wiley‐Liss, Inc.
Expression of Sox9, Mis, and Dmrt1 in the gonad of a species with temperature‐dependent sex determination
Authors: Shoemaker Christina , Ramsey Mary , Queen Joanna , Crews David 
Published: February 2007

AbstractSex determination in vertebrates, the process of forming an ovary or testis from a bipotential gonad, can be initiated by genetic or environmental factors. Elements of the downstream molecular pathways underlying these different sex‐determining mechanisms have been evolutionarily conserved. We find the first evidence that Sox9 expression is preferentially organized in the testis early in the temperature‐sensitive period in a species with temperature‐dependent sex determination (Trachemys scripta). This pattern occurs before sexually dimorphic Mis expression and in a temporal hierarchy that is similar to mammals. Furthermore, we extend previous findings that Dmrt1 expression at early stages of sex determination has a dimorphic pattern consistent with a possible upstream role in determining the fate of the bipotential gonad. Developmental Dynamics 236:1055–1063, 2007. © 2007 Wiley‐Liss, Inc.
Conditional targeted cell ablation in zebrafish: A new tool for regeneration studies
Authors: Curado Silvia , Anderson Ryan M. , Jungblut Benno , Mumm Jeff , Schroeter Eric , Stainier Didier Y.R. 
Published: February 2007

AbstractConditional targeted cell ablation in zebrafish would greatly expand the utility of this genetic model system in developmental and regeneration studies, given its extensive regenerative capabilities. Here, we show that, by combining chemical and genetic tools, one can ablate cells in a temporal‐ and spatial‐specific manner in zebrafish larvae. For this purpose, we used the bacterial Nitroreductase (NTR) enzyme to convert the prodrug Metronidazole (Mtz) into a cytotoxic DNA cross‐linking agent. To investigate the efficiency of this system, we targeted three different cell lineages in the heart, pancreas, and liver. Expression of the fusion protein Cyan Fluorescent Protein–NTR (CFP‐NTR) under control of tissue‐specific promoters allowed us to induce the death of cardiomyocytes, pancreatic β‐cells, and hepatocytes at specific times. Moreover, we have observed that Mtz can be efficiently washed away and that, upon Mtz withdrawal, the profoundly affected tissue can quickly recover. These findings show that the NTR/Mtz system is effective for temporally and spatially controlled cell ablation in zebrafish, thereby constituting a most promising genetic tool to analyze tissue interactions as well as the mechanisms underlying regeneration. Developmental Dynamics 236:1025–1035, 2007. © 2007 Wiley‐Liss, Inc.
The Magnetic Structure of Mn2GeSe4
Authors: Mikus Holger , Deiseroth Hans‐Jörg , Aleksandrov Krassimir , Ritter Clemens , Kremer Reinhard K. 
Published: February 2007

AbstractThe magnetic structure of the olivine type Mn2GeSe4 is investigated by powder neutron diffraction between 2 K and ambient temperature. Based on a number of “magnetic reflections” occurring below Tc = 66 K a model for the magnetic structure of the Mn2+ ions (d5) is derived by structure refinements based on the FULLPROF program system. Most important result is an antiferromagnetic arrangement of the Mn2+ moments in successive layers along [001] (“easy direction”). This ordering evolves gradually with decreasing temperature as can be seen from the temperature dependence of the resultant magnetization of the two magnetic sublattices. The magnetization direction of one of the magnetic sublattices shows a weak tendency at low temperatures to deviate from the easy direction [001] towards [100]. The magnetic structure proposed for the title compound is comparable with the ones found for other magnetic thio‐ and seleno‐olivines although a comprehensive picture for the magnetic properties of this group of solids is still lacking. The explanation for the “weak ferromagnetism” below Tc = 66 K with a saturation magnetization of about 0.5 μB per Mn2+ ion in a field of 7 T (2 K) as derived from earlier susceptibility measurements remains still speculative. It may result from local non‐zero components that do not show up in the overall magnetic structure. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Reactivity of Ruthenium(II) and Copper(I) Complexes that Possess Anionic Heteroatomic Ligands: Synthetic Exploitation of Nucleophilicity and Basicity of Amido, Hydroxo, Alkoxo, and Aryloxo Ligands for the Activation of Substrates that Possess Polar Bonds as well as Nonpolar C–H and H–H Bonds
Authors: Gunnoe T. Brent 
Published: February 2007

AbstractThe preparation and reactivity of late transition‐metal complexes in low oxidation states with nondative‐heteroatomic ligands (e.g., amido, hydroxo, alkoxo, and aryloxo ligands) are described. For such complexes the disruption of ligand‐to‐metal π‐donation because of a filled dπ manifold can enhance the nucleophilic and/or basic reactivity at the nondative‐heteroatomic ligand relative to transition‐metal complexes in high oxidation states. The chemistry of five‐ and six‐coordinate Ru complexes with amido, hydroxo, methoxo, and aryloxo ligands is described including Brønsted acid/base reactions, coordination, and activation of polar substrates toward N–C bond forming reactions and metal‐mediated activation of dihydrogen and C–H bonds. The impact of ancillary ligands and Ru oxidation states (RuII versus RuIII) are discussed. In addition, the preparation and reactivity of well‐defined monomeric two‐ and three‐coordinate Cu complexes with amido, alkoxo, hydroxo, and aryloxo ligands are presented including examples of reactions to probe fundamental reactivity patterns as well as catalytic addition of N–H and O–H bonds across C=C bonds of olefins. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Ruthenium Complex Fragments as Constituents of Trinuclear Photoactive Supramolecular Assemblies Based on Hydrogen Bond Association
Authors: Halbauer Kathi , Göbel Angela , Sterzik Anke , Görls Helmar , Rau Sven , Imhof Wolfgang 
Published: February 2007

AbstractThe reaction of the octahedral complexes cis‐ or trans‐[Ru(C≡N‐tBu)4(CN)2], (Ru(CN)2), with the photochemically active [bis(bipyridyl)bibenzimidazole‐ruthenium(II)], (RubiH2), component via hydrogen bond association results in the formation of supramolecular aggregates with modified photophysical properties. The first structural characterization of a supramolecular adduct is reported, in which the N–H functions of the ruthenium bound bibenzimidazole ligand via hydrogen bonds serve as an interface directly toward a cyanide ligand acting as a bridge to another transition metal. In addition, the maximum stoichiometry of the supramolecular associates is estimated by NMR titration experiments and association constants of the hydrogen bonded adducts are determined by the decay of the emission intensity during the titration experiments.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Spectroelectrochemical Sensing Based on Multimode Selectivity Simultaneously Achievable in a Single Device. 19. Preparation and Characterization of Films of Quaternized Poly(4‐vinylpyridine)‐silica
Authors: Conklin Sean D. , Heineman William R. , Seliskar Carl J. 
Published: February 2007

AbstractQuaternized poly(4‐vinylpyridine) (QPVP) has been incorporated as an anion exchanger into sol‐gel derived silica films for use in a spectroelectrochemical sensor. The preparation, characteristics and performance of these films are described. The films, which are spin‐coated onto the surface of a planar optically transparent electrode, are optically transparent and uniform. Scanning electron microscopy and spectroscopic ellipsometry have been used to examine film structure, thickness and optical properties. These films have been shown both spectroscopically and electrochemically to preconcentrate ferrocyanide, a model analyte for the sensor. The films can be regenerated for multiple measurements by exposure to 1 M KNO3. The effects of polymer molecular weight and storage conditions on film performance are described. The overall response of this film is comparable to the poly(dimethyldiallylammonium chloride)‐silica films previously used for this sensor.
Detailed analysis of loss of heterozygosity on chromosome band 17p13 in breast carcinoma on the basis of a high‐resolution physical map with 29 markers
Authors: Isomura Minoru , Tanigami Akira , Saito Hiroko , Harada Yosuke , Katagiri Toyomasa , Inazawa Joji , Nakamura Yusuke , Ledbetter David H. 
Published: February 2007

AbstractWe have constructed a physical map of chromosome band 17p 13, using 29 markers that had been localized to 17p 13 by means of fluorescence in situ hybridization (FISH) and analysis by pulsed‐field gel electrophoresis (PFGE). The map spans nearly 8 Mb of genomic DNA, and the estimated average distance between each marker is roughly 290 kb. The p 13 band of chromosome 17 is thought to contain a putative tumor suppressor gene in addition and distal to TP53. Deletion mapping in a large number of breast carcinomas indicated that the tumor suppressor gene lies between the loci defined by cC117‐708 (D17S878) and p144D6 (D17S34), which are an estimated 7 Mb apart. Our results should contribute to construction of a contig map of chromosome band 17p 13 with cosmid and/or YAC (yeast artificial chromosome) clones, and to isolation of the putative tumor suppressor gene. Genes Chrom Cancer 9:173‐179 (1994). © 1994 Wiley‐Liss, Inc.
A t( 10; 17) translocation creates the RET/PTC2 chimeric transforming sequence in papillary thyroid carcinoma
Authors: Sozzi Gabriella , Bongarzone Ltalia , Miouo Monica , Borrello Maria Grazia , Butti Marta Giaele , Porta Giuseppe Della , Pierotti Marco A. , Pilotti Silvana 
Published: February 2007

AbstractActivation of the RET protooncogene tyrosine kinase (tk) by fusion with other genes is a frequent finding in papillary thyroid carcinoma. The tk domain of proto‐RET can be fused either with the D10S170 gene generating the RET/PTCI transforming sequence or with sequences belonging to the gene encoding the regulatory subunit R/A of c‐AMP‐dependent protein kinase A, thus forming the RET/PTC2 oncogene. We have previously shown that an inversion of chromosome 10, inv(10)(q11.2q21), is responsible for the generation of the RET/PTCI. Here we report that a chromosomal translocation, t(10;17)(q11.2;q23), juxtaposes the tk domain of the RET protooncogene, which resides on chromosome 10, to a 5′ portion of the R/A gene on chromosome 17, leading to the formation of the chimeric transforming gene RET/PTC2. The finding of the transforming protein in primary tumor cell extracts supports the conclusion that RET/PTC2 activation plays a role in papillary thyroid tumorigenesis. Genes Chrom Cancer 9:244‐250 (1994). © 1994 Wiley‐Liss, Inc.
Inversion‐associated translocations in acute myelomonocytic leukemia with eosinophilia
Authors: Berger Roland , Derré Josette , Coniat Maryvonne Le , Hébert Josette , Serge Romana P. , Jonveaux Philippe 
Published: February 2007

AbstractCytogenetic studies of three acute myelomonocytic leukemias with bone marrow eosinophilia (M4EO) revealed chromosome 16 inversion associated with additional abnormalities. The inverted chromosome 16 was involved in two patients. Fluorescence in situ hybridization (FISH) experiments with a YAC probe detecting inv(16) showed that the translocation breakpoints involving chromosome 16 did not implicate the inversion breakpoints. FISH can thus distinguish between true variant translocations and translocations with other breakpoints on chromosome 16 in M4EO.
Translocation t(3;12)(q28;q14) in parosteal lipoma
Authors: Bridge Julia A. , Deboer Joanne , Walker Craig W. , Neff James R. 
Published: February 2007

AbstractParosteal lipoma is a rare primary benign bone tumor demonstrating histopathologic features similar to those seen in the commonly occurring lipoma of soft tissue. Cytogenetic studies of soft tissue lipoma have demonstrated frequent abnormalities of 12q13–15. To the best of our knowledge the cytogenetic findings in parosteal lipoma have not yet been described. Cytogenetic analysis of a parosteal lipoma of the femur of a 51‐year‐old female revealed a t(3;12)(q28;q14), indicating that bone and soft tissue lipomas are cytogenetically similar and likely share a common histopathogenesis.
Translocation t(8;13)(p11;q11‐12) in stem cell leukemia/lymphoma of t‐cell and myeloid lineages
Authors: Naeem Rizwan , Singer Samuel , Fletcher Jonathan A. 
Published: February 2007

AbstractAn unusual hematologic neoplasia has been described recently in which the predominant clinical features include T‐cell lymphoma, myeloid hyperplasia, and eosinophilia. The multilineage involvement in this disorder suggests transformation of a primitive stem cell. Abnormal karyotypes have been described in three such cases, including one case with t(8;13)(p11.2;q12) and a second case with t(8;13)(p23;q14). We report translocation of chromosomes 8 and 13 in lymph node karyotypes from two patients with this syndrome. Fluorescence in situ hybridization confirmed an identical translocation, t(8;13)(p11;q11‐12), in lymphoma cells from each patient. The translocation breakpoints are of particular interest because the FLT3 receptor tyrosine kinase gene has been mapped to 13q12. FLT3 is expressed highly in hematopoietic progenitor cells and in myeloid and lymphoid acute leukemias.