bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new approach of nano-metformin as a protector against radiation-induced cardiac fibrosis and inflammation via CXCL1/TGF-Β pathway
Authors: Karam Heba M. , Lotfy Dina M. , A. Ibrahim Ayman , Mosallam Farag M. , Abdelrahman Sahar S. , Abd-ElRaouf Amira 
Published: April 2024

AbstractThe present work investigates the potential role of metformin nanoparticles (MTF-NPs) as a radio-protector against cardiac fibrosis and inflammation induced by gamma radiation via CXCL1/TGF-β pathway. Lethal dose fifty of nano-metformin was determined in mice, then 21 rats (male albino) were equally divided into three groups: normal control (G1), irradiated control (G2), and MTF-NPs + IRR (G3). The possible protective effect of MTF-NPs is illustrated via decreasing cardiac contents of troponin, C-X-C motif Ligand 1 (CXCL1), tumor growth factor β (TGF-β), protein kinase B (AKT), and nuclear factor-κB (NF-κB). Also, the positive effect of MTF-NPs on insulin-like growth factor (IGF) and platelet-derived growth factor (PDGF) in heart tissues using immunohistochemical technique is illustrated in the present study. Histopathological examination emphasizes the biochemical findings. The current investigation suggests that MTF-NPs might be considered as a potent novel treatment for the management of cardiac fibrosis and inflammation in patients who receive radiotherapy or workers who may be exposed to gamma radiation.
Graphical Abstract
Recent advances in near-infrared I/II persistent luminescent nanoparticles for biosensing and bioimaging in cancer analysis
Authors: Chan Ming-Hsien , Chang Yu-Chan 
Published: April 2024

AbstractPhotoluminescent materials (PLNs) are photoluminescent materials that can absorb external excitation light, store it, and slowly release it in the form of light in the dark to achieve long-term luminescence. Developing near-infrared (NIR) PLNs is critical to improving long-afterglow luminescent materials. Because they excite in vitro, NIR-PLNs have the potential to avoid interference from in vivo autofluorescence in biomedical applications. These materials are promising for biosensing and bioimaging applications by exploiting the near-infrared biological window. First, we discuss the biomedical applications of PLNs in the first near-infrared window (NIR-I, 700–900 nm), which have been widely developed and specifically introduce biosensors and imaging reagents. However, the light in this area still suffers from significant light scattering and tissue autofluorescence, which will affect the imaging quality. Over time, fluorescence imaging technology in the second near-infrared window (NIR-II, 1000–1700 nm) has also begun to develop rapidly. NIR-II fluorescence imaging has the advantages of low light scattering loss, high tissue penetration depth, high imaging resolution, and high signal-to-noise ratio, and it shows broad application prospects in biological analysis and medical diagnosis. This critical review collected and sorted articles from the past 5 years and introduced their respective fluorescence imaging technologies and backgrounds based on the definitions of NIR-I and NIR-II. We also analyzed the current advantages and dilemmas that remain to be solved. Herein, we also suggested specific approaches NIR-PLNs can use to improve the quality and be more applicable in cancer research.
Graphical abstract
In-line coupling of capillary-channeled polymer fiber columns with optical absorbance and multi-angle light scattering detection for the isolation and characterization of exosomes
Authors: Wysor Sarah K. , Marcus R. Kenneth 
Published: April 2024

AbstractExtracellular vesicles (EVs) have garnered much interest due to their fundamental role in intracellular communication and their potential utility in clinical diagnostics and as biotherapeutic vectors. Of particular relevance is the subset of EVs referred to as exosomes, ranging in size from 30 to 150 nm, which contain incredible amounts of information about their cell of origin, which can be used to track the progress of disease. As a complementary action, exosomes can be engineered with therapeutic cargo to selectively target diseases. At present, the lack of highly efficient methods of isolation/purification of exosomes from diverse biofluids, plants, and cell cultures is a major bottleneck in the fundamental biochemistry, clinical analysis, and therapeutic applications. Equally impactful, the lack of effective in-line means of detection/characterization of isolate populations, including concentration and sizing, is limiting in the applications. The method presented here couples hydrophobic interaction chromatography (HIC) performed on polyester capillary-channeled polymer (C-CP) fiber columns followed by in-line optical absorbance and multi-angle light scattering (MALS) detection for the isolation and characterization of EVs, in this case present in the supernatant of Chinese hamster ovary (CHO) cell cultures. Excellent correlation was observed between the determined particle concentrations for the two detection methods. C-CP fiber columns provide a low-cost platform (< $5 per column) for the isolation of exosomes in a 15-min workflow, with complementary absorbance and MALS detection providing very high-quality particle concentration and sizing information.
Sonographic diagnosis of spondylodiscitis in a young child
Authors: Soudack Michalle , Shimoni Hadar-Yafit , Plotkin Simyon , Jacobson Jeffrey M 
Published: April 2024

AbstractSonographic diagnosis of spondylodiscitis is described in a 21-month-old girl who presented with altered gait. Spondylodiscitis, also referred to as discitis-osteomyelitis, is an infection of the intervertebral disc and adjacent vertebrae. The imaging modality of choice is spinal magnetic resonance imaging. Our case is the first description in the English language of the sonographic diagnosis of spondylodiscitis. Pediatric radiologists and sonographers should be acquainted with its features, for both incidental and intentional diagnosis.
Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites
Authors: Luo Dewei , Yan Li , Wang Zhujun , Ji Xiaofan , Pei Na , Jia Jing , Luo Yingying , Ouyang Hui , Yang Shilin , Feng Yulin 
Published: April 2024

Abstract
Pulchinenoside B4, a natural saponin monomer from the Pulsatilla plant, plays an important role as an immunomodulator in the treatment of acute inflammation. Oral ulcer (OU) is a common ulcerative injury disease that occurs in the oral mucosa, including mucosal ulceration and abnormalities of lips and tongue. A close correlation exists between gut microbiota and circulating metabolites in patients with OU. However, the correlation between gut microbiota and serum metabolomics is not clear. Therefore, this study aimed to explore the changes in gut microbiota and metabolites in OU. The 16S ribosomal RNA (16S rRNA) gene sequencing was used to detect the changes in the composition of gut microbiota in OU rat model. Moreover, the endogenous small metabolites were explored by collecting the non-targeted serum metabolomics data. A total of 34 OU-related biomarkers were identified, mainly related to fatty acid metabolism and inflammatory pathways. The administration of B4 effectively reduced the occurrence of OU and restored the levels of multiple endogenous biomarkers and key gut microbial species to the normal level. This study demonstrated that the gut microbiota and metabolites were altered in the OU rat model, which were significantly restored to the normal level by B4, thereby showing good application prospects in the treatment of OU.

Key points
• The first investigating the correlation between OU and gut microbiota.
• A close correlation between metabolites and gut microbiota in OU disease was successfully identified.
• Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites.
Modulating Growth and Oil Profile of Sesame (Sesamum indicum L.): Paclobutrazol and Mepiquat Chloride Impacts
Authors: Qureshi Moin , Kordrostami Mojtaba , Uzun Bulent , Yol Engin 
Published: April 2024

AbstractSesame (Sesamum indicum L.) is an important oilseed crop with significant economic importance in many developing countries. This study rigorously investigated the impact of various applications and doses of two specific plant growth regulators, paclobutrazol (PAC) and mepiquat chloride (MC), on the development, yield components, oil content, and fatty acid composition of sesame plants across two consecutive growing seasons. The research revealed that while paclobutrazol application increased the plant height it also resulted in a slight decrease in oil content. The paclobutrazol treatment resulted in the highest seed yield (21.3 g/plant), which was significantly different from both the MC (13.1 g/plant) and PAC + MC (14.6 g/plant) treatments. In contrast, the mepiquat chloride treatment resulted in the lowest oil content. The combined application of both regulators showed intermediate effects. In addition, variations in fatty acid content were observed between applications and doses, with significant differences in saturated and unsaturated fatty acid levels. The findings provide valuable insights into the potential benefits and implications of using growth regulators in sesame cultivation, especially in the context of mechanized harvesting and oil quality optimization.
Impacts of anthropogenic structures in long- and short-term shoreline evolution of Santa Marta Bay (Colombian Caribbean)
Authors: Portz Luana Carla , Villate-Daza Diego , Bolívar-Anillo Hernando José , Fontán-Bouzas Ángela , Alcántara-Carrió Javier , Manzolli Rogerio Portantiolo 
Published: April 2024

AbstractInteraction of Anthropic interventions (rigid structures) with coasts is an essential aspect for understanding their geomorphic evolution and incorporating these data into adequate coastal management. This study analyses the short and long-term behavior (1985 to 2019) of shoreline at Santa Marta Bay (Caribbean - Colombia) and their relationship with coastal protection structures. The shoreline variations were analyzed through aerial photographs and satellite images using DSAS tools. The short-term assessment showed initial intense 1985–1991 erosion, with an average retreat speed rate of − 1.6 m·y− 1. It changed gradually since 2003, due to the construction of rigid structures and beach nourishment, decreasing shoreline retreat and even a progradation rate of 0.2 m·y− 1 was observed. The 2009–2019 period, despite recording a positive average value, exhibited a high percentage of erosion profiles. Therefore, in a decadal or long-term analysis (1985–2019), despite engineering works present positive results in the short term, new interventions are required. The coastal erosion is not uniform along the shoreline, because the area is a closed bay with small sediment inputs, the variations in erosion and accretion rates change with human intervention and the installation of new structures. Therefore, quantifying the scale and rate of shoreline changes and correlating them with anthropogenic structures is an essential step in assessing shoreline vulnerability.
Lymphangioleiomyomatosis and pregnancy: a mini-review
Authors: Zhou Jieshu , Diao Min 
Published: April 2024

AbstractLymphangioleiomyomatosis(LAM) is a slow progressive, rare cystic lung disease in women of reproductive age, associated with infiltration of the lung by atypical smooth muscle like cells, leading to the cystic destruction of the lung parenchyma. As LAM exclusively affects women of childbearing age, it can arise or exacerbate during pregnancy. Many patients with LAM are discouraged from pregnancy, although there is not much objective evidence effect on fertility. Patients diagnosed with LAM during pregnancy experience worse outcomes, so the safety of pregnancy is a vexing problem. What was worse, treatment strategies are limited on the effects of LAM on pregnancy outcomes. Pregnancy could be considered in LAM patients. Successful delivery in women with LAM depends on the condition of the LAM, which is in turn dependent on obstetricians and respiratory physicians. In this review, we describe the epidemiology, pathogenesis, diagnosis, clinical features and the treatment strategies of LAM during pregnancy.
Experimental re-melting of a continental crust: probing the deep storage zone of Campi Flegrei and Vesuvius magmas
Authors: Palummo Flavia , Perinelli Cristina , Bonechi Barbara , Fabbrizio Alessandro , Misiti Valeria , Scarlato Piergiorgio , Gaeta Mario 
Published: April 2024

AbstractMantle magmas interact with surrounding rocks during their ascent and storage in the continental crust, leading to open system processes as wall rock partial melting. In this study, we have experimentally investigated the reactions between a leucosome depleted migmatite and a primitive K-basaltic of Campi Flegrei (Italy). Experiments were carried out at pressure of 0.8 GPa temperatures from 1250 °C to 1050 °C and constant temperature and thermal gradient conditions. The experimental products consist of biotite-free migmatite, glass and crystals of clinopyroxene, olivine, plagioclase and Cr-spinel with proportions that vary as a function of temperature. Open system isothermal experiments indicate that the chemistry of melts and phase relationships are controlled by the high Al2O3 content of leucosome depleted migmatite with the glass composition shifting from K-trachybasalt towards shoshonite as the temperature decreases from 1200 °C to 1125 °C. At temperatures $$\le$$1150°C, migmatite assimilation is not exclusively due to the assimilation fractional crystallization process because evidence of mingling and mixing is observed. T-gradient experiment shows melt composition ranging from shoshonite to phono-tephrite moving from the slightly crystalline zone (T = 1250 –1210 °C) at the bottom of the capsule towards the highly crystalline zone (T = 1160 –1140 °C). This SiO2-constant trend indicates that at temperature below the basalt solidus, the assimilation of leucosome depleted crust is represented almost exclusively by the biotite breakdown, leading to the increase in Al, Mg, Fe, Ti, and K activities in the system. The shoshonitic composition obtained in our experiments could represent the parental magma for both Campi Flegrei volcanic district and Vesuvius magmatic systems, indicating modification in a deep storage zone through mixing with the partial melts derived from restitic continental crust.
Test–retest reliability of lower limb muscle strength, jump and sprint performance tests in elite female team handball players
Authors: Fristrup Bjørn , Krustrup Peter , Kristensen Kevin Højer , Rasmussen Steffen , Aagaard Per 
Published: April 2024

Abstract
Purpose
This study aimed to assess the reliability of lower limb muscle function (knee extensor/flexor peak torque, rate of torque development (RTD), impulse, and countermovement jump (CMJ) performance) and sprint performance (acceleration capacity).

Methods
CMJ performance was evaluated on a force plate. MVIC, RTD and impulse variables were investigated using a portable isometric dynamometer and sprint performance was assessed with dual-beam photocells in elite female athletes.

Results
CMJ test variables maximal vertical jump height, peak and mean power, concentric work, and body center of mass displacement demonstrated good-to-excellent test–retest correlations between Test 1 and Test 2 (ICC ≥ 0.70, CWw-s = 3.4–11.0%). Peak MVIC torque for the knee extensors and flexors demonstrated excellent test–retest correlations (both ICC = 0.84) along with CVw-s values of 6.8 and 6.0%, respectively. Late-phase (0–100 ms, 0–200 ms) RTD for the knee flexors demonstrated excellent test–retest correlations (ICC = 0.89–0.91, CVw-s = 4.8–8.5%). Sprint times at 10- and 20-m demonstrated excellent test–retest reproducibility (ICC = 0.83 and ICC = 0.90, respectively) with CVw-s values of 1.9 and 1.5%. For 5-m sprint times, test–retest reproducibility was good (ICC = 0.71) with CVw-s of 2.8%. Sprint testing performed while dribbling a handball improved (p < 0.05) from test to retest at 5-, 10- and 20-m.

Conclusion
In conclusion, the force plate, the mobile isometric dynamometer, and dual-beam photocells provide reproducible tools for field-based testing of countermovement jump performance, knee extensor and flexor strength and sprint performance.
Quantification of fluorescence angiography for visceral perfusion assessment: measuring agreement between two software algorithms
Authors: Nijssen D. J. , Joosten J. J. , Osterkamp J. , van den Elzen R. M. , de Bruin D. M. , Svendsen M. B. S. , Dalsgaard M. W. , Gisbertz S. S. , Hompes R. , Achiam M. P. , van Berge Henegouwen M. I. 
Published: April 2024

Abstract
Background
Indocyanine green fluorescence angiography (ICG-FA) may reduce perfusion-related complications of gastrointestinal anastomosis. Software implementations for quantifying ICG-FA are emerging to overcome a subjective interpretation of the technology. Comparison between quantification algorithms is needed to judge its external validity. This study aimed to measure the agreement for visceral perfusion assessment between two independently developed quantification software implementations.

Methods
This retrospective cohort analysis included standardized ICG-FA video recordings of patients who underwent esophagectomy with gastric conduit reconstruction between August 2020 until February 2022. Recordings were analyzed by two quantification software implementations: AMS and CPH. The quantitative parameter used to measure visceral perfusion was the normalized maximum slope derived from fluorescence time curves. The agreement between AMS and CPH was evaluated in a Bland–Altman analysis. The relation between the intraoperative measurement of perfusion and the incidence of anastomotic leakage was determined for both software implementations.

Results
Seventy pre-anastomosis ICG-FA recordings were included in the study. The Bland–Altman analysis indicated a mean relative difference of + 58.2% in the measurement of the normalized maximum slope when comparing the AMS software to CPH. The agreement between AMS and CPH deteriorated as the magnitude of the measured values increased, revealing a proportional (linear) bias (R2 = 0.512, p < 0.001). Neither the AMS nor the CPH measurements of the normalized maximum slope held a significant relationship with the occurrence of anastomotic leakage (median of 0.081 versus 0.074, p = 0.32 and 0.041 vs 0.042, p = 0.51, respectively).

Conclusion
This is the first study to demonstrate technical differences in software implementations that can lead to discrepancies in ICG-FA quantification in human clinical cases. The possible variation among software-based quantification methods should be considered when interpreting studies that report quantitative ICG-FA parameters and derived thresholds, as there may be a limited external validity.
Umsetzbarkeit des interdisziplinären multimodalen Assessments – die Teamperspektive
Authors: Schouten Leonie , Petzke Frank , Gärtner Anne , Nagel Bernd ,   PAIN2020-Team, Isenberg Thomas , Lindena Gabriele , Martin Carolin , Möller André , Augustin Katharina , Kaiser Ulrike , Gärtner Anne , Preißler Anke , Hoffmann Greta , Pritzke Michael Julia , Petzke Frank , Pfingsten Michael , Schouten Leonie , Deppe Karin , Casser Hans-Raimund , Nagel Bernd , Schwenk Katja , Metz-Oster Beatrice , Milch Lena , Rensland Jana , Kohlmann Thomas , Szczotkowski Daniel , Marschall Ursula , Schumacher Catharina , Kaiser Ulrike 
Published: April 2024

Zusammenfassung
Hintergrund
Sekundärpräventive, ambulante Diagnostikangebote für Patient:innen mit Schmerzen und Risikofaktoren für eine Chronifizierung sind bisher nicht hinreichend etabliert. Im Projekt PAIN2020 (Innovationsfonds, 01NVF17049) wurde erstmalig ein frühzeitig im Krankheitsverlauf ansetzendes, ambulantes interdisziplinäres multimodales Assessment (IMA) eingeführt.

Ziel
Zur Durchführung des IMA wurden Abläufe zur Teamzusammenarbeit und Entscheidungskriterien entwickelt, die durch ein Team aus medizinischen, physiotherapeutischen und psychologischen Therapeut:innen umgesetzt wurden. Diese Abläufe und Entscheidungskriterien sollen vor dem Hintergrund klinischer Erfahrung diskutiert und hinsichtlich ihrer Umsetzbarkeit (qualitativ) überprüft werden.

Methodik
Im September 2021 fand ein Workshop zum IMA in PAIN2020 statt, um die im Prozess bisher gewonnenen Erkenntnisse und Erfahrungen durch das Monitoring und die strukturierende Dokumentation in der Umsetzung mit Mitarbeitenden bzw. Teams der PAIN2020-Zentren zur Umsetzbarkeit eines strukturierten interdisziplinären multimodalen Assessments gemeinsam zu reflektieren. In drei Arbeitsphasen wurden berufsgruppenspezifische und -übergreifende Themen bearbeitet.

Ergebnisse
In den Entscheidungsprozessen der Berufsgruppen zeigen sich neben professionsspezifischen Schwerpunkten im Rahmen der Befunderhebung (somatische, funktionelle oder psychosoziale Kernkriterien) jeweils übergreifende Kernkriterien innerhalb der Professionen sowie ergänzend patientenbezogene Aspekte, die in den integrativen Teamprozess einbezogen werden. Hinsichtlich der Teamzusammenarbeit lassen sich aus der Umsetzung der Teamsitzung und des Abschlussgesprächs fördernde bzw. hemmende Struktur- und Prozessparameter in der Umsetzung identifizieren, die auch durch interaktionelle Faktoren begleitet werden.

Diskussion
Für die Umsetzung des IMA ergaben sich (1) Anpassungen für das IMA, das derzeit als A‑IMA im Selektivvertrag mit der BARMER umgesetzt wird, und (2) neue Dimensionen bzw. Aufgabenfelder und Ideen für evidenzbasierte Konzepte zur inhaltlichen Ausgestaltung integrativer Diagnostik sowie für die Rückmeldung der Ergebnisse an die Patient:innen, die zukünftig diskutiert werden sollten.

Graphic abstract
Nanocomposite microbeads made of recycled polylactic acid for the magnetic solid phase extraction of xenobiotics from human urine
Authors: Antonelli Lorenzo , Frondaroli Maria Chiara , De Cesaris Massimo Giuseppe , Felli Nina , Dal Bosco Chiara , Lucci Elena , Gentili Alessandra 
Published: April 2024

AbstractNanocomposite microbeads (average diameter = 10–100 µm) were prepared by a microemulsion-solidification method and applied to the magnetic solid-phase extraction (m-SPE) of fourteen analytes, among pesticides, drugs, and hormones, from human urine samples. The microbeads, perfectly spherical in shape to maximize the surface contact with the analytes, were composed of magnetic nanoparticles dispersed in a polylactic acid (PLA) solid bulk, decorated with multi-walled carbon nanotubes (mPLA@MWCNTs). In particular, PLA was recovered from filters of smoked electronic cigarettes after an adequate cleaning protocol. A complete morphological characterization of the microbeads was performed via Fourier-transform infrared (FTIR) spectroscopy, UV–Vis spectroscopy, thermogravimetric and differential scanning calorimetry analysis (TGA and DSC), scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD). The recovery study of the m-SPE procedure showed yields ≥ 64%, with the exception of 4-chloro-2-methylphenol (57%) at the lowest spike level (3 µg L−1). The method was validated according to the main FDA guidelines for the validation of bioanalytical methods. Using liquid chromatography-tandem mass spectrometry, precision and accuracy were below 11% and 15%, respectively, and detection limits of 0.1–1.8 µg L−1. Linearity was studied in the range of interest 1–15 µg L−1 with determination coefficients greater than 0.99. In light of the obtained results, the nanocomposite microbeads have proved to be a valid and sustainable alternative to traditional sorbents, offering good analytical standards and being synthetized from recycled plastic material. One of the main objectives of the current work is to provide an innovative and optimized procedure for the recycling of a plastic waste, to obtain a regular and reliable microstructure, whose application is here presented in the field of analytical chemistry. The simplicity and greenness of the method endows the procedure with a versatile applicability in different research and industrial fields.
Graphical Abstract