bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of ultra‐early, early, and normal soybean planting dates and rye cover crop on soybean grain yield
Authors: Kannberg Seth , Lindsey Alexander J. , Chiavegato Marília B. , Lindsey Laura E. 
Published: February 2024

AbstractWith changes to climate and crop insurance, earlier soybean [Glycine max (L.) Merr.] planting dates need to be investigated. Additionally, the use of a cover crop prior to soybean is promoted as a sustainable practice though little is known about cover crop and ultra‐early soybean planting (prior to April 15). The objective was to evaluate soybean planting date and cereal rye (Secale cereale L.) cover crop termination timing on cover crop biomass, soybean plant population, and yield. The study was conducted in Northeast and West Central Ohio in 2021 and 2022. Treatments included three soybean planting dates (early April, late April, and late May) and three cover crop treatments (termination 1–2 weeks prior to planting or “early,” termination at or after planting or “late,” and no cover crop). Cover crop biomass increased as termination was delayed. Soybean planted in early April resulted in a yield reduction of 1.8 Mg ha−1 when planted into a cover crop compared to the no cover crop control. However, when soybean was planted in late April, grain yield was not different among cover crop treatments. Yield reduction associated with early April planting with a cover crop was likely due to low soybean plant population, especially in Northeast Ohio, where plant population was <55,000 plants ha−1 with a cover crop and >120,000 plants ha−1 without a cover crop. To maximize yield, soybean should be planted by the end of April in Northeast Ohio. In West Central Ohio, soybean can be planted in early April without a cover crop.
Intestinal microbiota composition and efficacy of iron supplementation in Peruvian children
Authors: Dorsey Achsah F. , Roach Jeff , Burten Rachel B. , Azcarate‐Peril M. Andrea , Thompson Amanda L. 
Published: February 2024

AbstractObjectiveDespite repeated public health interventions, anemia prevalence among children remains a concern. We use an evolutionary medicine perspective to examine the intestinal microbiome as a pathway underlying the efficacy of iron‐sulfate treatment. This study explores whether gut microbiota composition differs between anemic children who respond and do not respond to treatment at baseline and posttreatment and if specific microbiota taxa remain associated with response to iron supplementation after controlling for relevant inflammatory and pathogenic variables.MethodsData come from 49 pre‐school‐aged anemic children living in San Juan de Lurigancho, Lima, Peru. We tested for differences in alpha and beta diversity using QIIME 2 and performed differential abundance testing in DESeq2 in R. We ran multivariate regression models to assess associations between abundance of specific taxa and response while controlling for relevant variables in Stata 17.ResultsWhile we found no evidence for gut microbiota diversity associated with child response to iron treatment, we observed several differential abundance patterns between responders and non‐responders at both timepoints. Additionally, we present support for a nonzero relationship between lower relative abundance of Barnesiellaceae and response to iron supplementation in samples collected before and after treatment.ConclusionWhile larger studies and more specific approaches are needed to understand the relationship between microbes and anemia in an epidemiological context, this study suggests that investigating nutritional status and pathogen exposure is key to better understanding the gut microbiome and impact of iron fortification.
Synthese an der Wasseroberfläche von Vinylen‐Verbundenen Kationischen Zwei‐Dimensionalen Polymerfilmen als Beschichtung für Anion‐Selektive Elektroden
Authors: Yang Ye , Sabaghi Davood , Liu Chang , Dianat Arezoo , Mücke David , Qi Haoyuan , Liu Yannan , Hambsch Mike , Xu Zhi‐Kang , Yu Minghao , Cuniberti Gianaurelio , Mannsfeld Stefan C. B. , Kaiser Ute , Dong Renhao , Wang Zhiyong , Feng Xinliang 
Published: February 2024

AbstractVinylen verbrückte zwei‐dimensionale Polymere (V‐2DPs) und deren schichtgestapelten kovalent organische Gerüstverbindungen (V‐2D COFs) zeichnen sich durch ihre hohe π‐Konjugation in der Ebene und ihre robuste Struktur als vielversprechnende Kanidaten für energiebezogene Anwendungen aus. Allerdings sind derzeitige syntheisische Ansätze auf die Herstellung von V‐2D COF Pulver beschränkt, welche schlecht prozessierbar sind, was die Integration in Geräte, insbesondere für Membranthechnologien, welche auf dünnen Filmen beruhen erschwert. Im folgenden berichten wir über die neu‐artige Synthese von kationischen 2DPs‐Filmen an der Wasseroberfläche, die über Vinyleneinheiten verbrückt sind (V‐C2DP‐1 und V‐C2DP‐2) mittels Knoevenagel‐Polykondensation, diese dienen als anionenselektive Elektrodenbeschichtung für hochreversible und langlebige Zink‐basierte Dual‐Ionen‐Batterien (ZDIBs). Modellreaktionen und theoretische Modellierung zeigen eine erhöte Reaktivität und Reversibilität der Knoevenagel‐Reaktion an der Wasseroberfläche an. Basierend darauf zeigten wir die 2D Polykondensation hin zu V‐C2DP‐Filmen an der Wasseroberfläche, welche eine weitreichen‐de laterale Größe, anpassbare Dicke und hohe chemischer Stabilität besitzen. Repräsentativ zeigt sich V‐C2DP‐1 als ein vollständig crystalliner und flächen‐orientierter Film mit den in der Ebene liegenden Gitterparametern von a=b≈43.3 Å. Dank der gut definierten katinoischen Funktionalitäten, orientierten 1D‐Kanälen und des sabilen Gerüstes besitzt der V‐C2DP‐1 Film eine herausragende Transportselektivität von Bis(trifluormethansulfon)imid‐anion (TFSI−) (Transferrate, t =0.85) für die Graphitkathode in Hochspannungs‐ZDIBs. Die hohe Selektivität führt zu zusätzlichen TFSI−‐Intercalationsstufen wodurch eine höhere spezifischen Kapazität (von ~83 bis 124 mAh g−1), sowie Zyklusdauer (>1000 Zyklen, 95 % Kapazitätserhaltung) gefördert wird.
Palladium (II)‐Catalyzed C−H Activation with Bifunctional Ligands: From Curiosity to Industrialization
Authors: Wu Kevin , Lam Nelson , Strassfeld Daniel A. , Fan Zhoulong , Qiao Jennifer X. , Liu Tao , Stamos Dean , Yu Jin‐Quan 
Published: February 2024

AbstractIn 2001, our curiosity to understand the stereochemistry of C−H metalation with Pd prompted our first studies in Pd(II)‐catalyzed asymmetric C−H activation (RSC Research appointment: 020 7451 2545, Grant: RG 36873, Dec. 2002). We identified four central challenges: 1. poor reactivity of simple Pd salts with native substrates; 2. few strategies to control site selectivity for remote C−H bonds; 3. the lack of chiral catalysts to achieve enantioselectivity via asymmetric C−H metalation, and 4. low practicality due to limited coupling partner scope and the use of specialized oxidants. These challenges necessitated new strategies in catalyst and reaction development. For reactivity, we developed approaches to enhance substrate–catalyst affinity together with novel bifunctional ligands which participate in and accelerate the C−H cleavage step. For site‐selectivity, we introduced the concept of systematically modulating the distance and geometry between a directing template, catalyst, and substrate to selectively access remote C−H bonds. For enantioselectivity, we devised predictable stereomodels for catalyst‐controlled enantioselective C−H activation based on the participation of bifunctional ligands. Finally, for practicality, we have developed varied catalytic manifolds for Pd(II) to accommodate diverse coupling partners while employing practical oxidants such as simple peroxides. These advances have culminated in numerous C−H activation reactions, setting the stage for broad industrial applications.
Peptide‐Directed Attachment of Hydroxylamines to Specific Lysines of IgG Antibodies for Bioconjugations with Acylboronates
Authors: Tanriver Matthias , Müller Marco , Levasseur Mikail D. , Richards Daniel , Majima Sohei , DeMello Andrew , Yamauchi Yohei , Bode Jeffrey W. 
Published: February 2024

AbstractThe role of monoclonal antibodies as vehicles to deliver payloads has evolved as a powerful tool in cancer therapy in recent years. The clinical development of therapeutic antibody conjugates with precise payloads holds great promise for targeted therapeutic interventions. The use of affinity‐peptide mediated functionalization of native off‐the‐shelf antibodies offers an effective approach to selectively modify IgG antibodies with a drug–antibody ratio (DAR) of 2. Here, we report the traceless, peptide‐directed attachment of two hydroxylamines to native IgGs followed by chemoselective potassium acyltrifluoroborate (KAT) ligation with quinolinium acyltrifluoroborates (QATs), which provide enhanced ligation rates with hydroxylamines under physiological conditions. By applying KAT ligation to the modified antibodies, conjugation of small molecules, proteins, and oligonucleotides to off‐the‐shelf IgGs proceeds efficiently, in good yields, and with simultaneous cleavage of the affinity peptide‐directing moiety.
Bio‐inspired Two‐dimensional Nanofluidic Ionic Transistor for Neuromorphic Signal Processing
Authors: Mei Tingting , Liu Wenchao , Sun Fusai , Chen Yuanxia , Xu Guoheng , Huang Zijia , Jiang Yisha , Wang Senyao , Chen Lu , Liu Junjun , Fan Fengtao , Xiao Kai 
Published: February 2024

AbstractVoltage‐gated ion channels prevalent in neurons play important roles in generating action potential and information transmission by responding to transmembrane potential. Fabricating bio‐inspired ionic transistors with ions as charge carriers will be crucial for realizing neuro‐inspired devices and brain‐liking computing. Here, we reported a two‐dimensional nanofluidic ionic transistor based on a MXene membrane with sub‐1 nm interlayer channels. By applying a gating voltage on the MXene nanofluidic, a transmembrane potential will be generated to active the ionic transistor, which is similar to the transmembrane potential of neuron cells and can be effectively regulated by changing membrane parameters, e.g., thickness, composition, and interlayer spacing. For the symmetric MXene nanofluidic, a high on/off ratio of ~2000 can be achieved by forming an ionic depletion or accumulation zone, contingent on the sign of the gating potential. An asymmetric PET/MXene‐composited nanofluidic transitioned the ionic transistor from ambipolar to unipolar, resulting in a more sensitive gate voltage characteristic with a low subthreshold swing of 560 mV/decade. Furthermore, ionic logic gate circuits, including the “NOT”, “NAND”, and “NOR” gate, were implemented for neuromorphic signal processing successfully, which provides a promising pathway towards highly parallel, low energy consumption, and ion‐based brain‐like computing.
Cyclic Amine Synthesis via Catalytic Radical‐Polar Crossover Cycloadditions
Authors: Zhang Ying , Chen Shu‐Sheng , Li Kai‐Dian , Huang Huan‐Ming 
Published: February 2024

AbstractThe rapid assembly of valuable cyclic amine architectures in a single step from simple precursors has been recognized as an ideal platform in term of efficiency and sustainability. Although a vast number of studies regarding cyclic amine synthesis has been reported, new synthetic disconnection approaches are still high in demand. Herein, we report a catalytic radical‐polar crossover cycloaddition to cyclic amine synthesis triggered from primary sulfonamide under photoredox condition. This newly developed disconnection, comparable to established synthetic approaches, will allow to construct β, β‐disubstituted cyclic amine and β‐monosubstituted cyclic amine derivatives efficiently. This study highlights the unique utility of primary sulfonamide as a bifunctional reagent, which acts as a radical precursor and a nucleophile. The open‐shell methodology demonstrates broad tolerance to various functional groups, drug derivatives and natural products in an economically and sustainable fashion.
High‐Throughput Host–Microbe Single‐Cell RNA Sequencing Reveals Ferroptosis‐Associated Heterogeneity during Acinetobacter baumannii Infection
Authors: Meng Hongen , Zhang Tianyu , Wang Zhang , Zhu Yuyi , Yu Yingying , Chen Hangfei , Chen Jiaye , Wang Fudi , Yu Yunsong , Hua Xiaoting , Wang Yongcheng 
Published: February 2024

AbstractInteractions between host and bacterial cells are integral to human physiology. The complexity of host–microbe interactions extends to different cell types, spatial aspects, and phenotypic heterogeneity, requiring high‐resolution approaches to capture their full complexity. The latest breakthroughs in single‐cell RNA sequencing (scRNA‐seq) have opened up a new era of studies in host–pathogen interactions. Here, we first report a high‐throughput cross‐species dual scRNA‐seq technology by using random primers to simultaneously capture both eukaryotic and bacterial RNAs (scRandom‐seq). Using reference cells, scRandom‐seq can detect individual eukaryotic and bacterial cells with high throughput and high specificity. Acinetobacter baumannii (A.b) is a highly opportunistic and nosocomial pathogen that displays resistance to many antibiotics, posing a significant threat to human health, calling for discoveries and treatment. In the A.b infection model, scRandom‐seq witnessed polarization of THP‐1 derived‐macrophages and the intracellular A.b‐induced ferroptosis‐stress in host cells. The inhibition of ferroptosis by Ferrostatin‐1 (Fer‐1) resulted in the improvement of cell vitality and resistance to A.b infection, indicating the potential to resist related infections. scRandom‐seq provides a high‐throughput cross‐species dual single‐cell RNA profiling tool that will facilitate future discoveries in unraveling the complex interactions of host–microbe interactions in infection systems and tumor micro‐environments.
Cancer Cell‐Selective PD‐L1 Inhibition via a DNA Safety Catch to Enhance Immunotherapy Specificity
Authors: Bi Shiyi , Chen Wei , Fang Yanyun , Shen Jieyu , Zhang Qing , Guo Hongqian , Ju Huangxian , Liu Ying 
Published: February 2024

AbstractImmune checkpoint protein blockade (ICB) has emerged as a powerful immunotherapy approach, but suppressing immune‐related adverse events (irAEs) for noncancerous cells and normal tissues remains challenging. Activatable ICB has been developed with tumor microenvironment highly‐expressed molecules as stimuli, but they still lack precision and efficiency considering the diffusion of stimuli molecules in whole tumor tissue. Here we assemble PD−L1 with a duplex DNA strand, termed as “safety catch”, to regulate its accessibility for ICB. The safety catch remains at “on” status for noncancerous cells to prevent ICB binding to PD−L1. Cancer cell membrane protein c‐Met acts as a trigger protein to react with safety catch, which selectively exposes its hybridization region for ICB reagent. The ICB reagent is a retractable DNA nanostring with repeating hairpin‐structural units, whose contraction drives PD−L1 clustering with endocytosis‐guided degradation. The safety catch, even remained at “safety on” status, is removed from the cell membrane via a DNA strand displacement reaction to minimize its influence on noncancerous cells. This strategy demonstrates selective and potent immunotherapeutic capabilities only against cancer cells both in vitro and in vivo, and shows effective suppression of irAEs in normal tissues, therefore would become a promising approach for precise immunotherapy in mice.
High‐temperature resistant copolymer oil well cement retarder: Synthesis, assessment of performance, and mechanism of retardation
Authors: Bian Jifa , Ye Zhongbin , Zheng Xuecheng , Deng Chengwen , Tang Lei 
Published: February 2024

AbstractThis work used aqueous solution free radical polymerization to create polymers of 2‐acrylamide‐2‐methylpropane sulfonic acid, itaconic acid, and allyl polyoxyethylene ether as a high‐temperature resistant retarder (APMS/IA/APEG, from now on referred to as AIA). Gel permeation chromatography, fourier transform infrared (FTIR), proton nuclear magnetic resonance (1HNMR), and thermo‐gravimetric analysis (TG) were used to characterize their molecular structure and characteristics. Tests were conducted on the thickening time, rheological characteristics, and compressive strength of AIA in cement slurries. The cement slurry thickened for 276 min at 180°C during the test, and the cement stone's 24‐h compressive strength was consistently more than 20 MPa. The hydration products were analyzed using x‐ray diffractometer and scanning electron microscopy methods, which were then coupled with molecular dynamics simulations to elucidate the AIA retarding mechanism. When AIA is added to a cement slurry, it forms a chelate structure with Ca2+ and a strong adsorption layer on the surfaces of the cement particles. This stops the cement from hydrating. The AIA side chain, APEG, can wrap the active group in AIA at low temperatures. AIA exposes more adsorption groups because of the APEG chains stretching at higher temperatures. AIA can, therefore, be used for deep well operations at high temperatures.
Analyzing the interactions and miscibility of silk fibroin/mucin blends
Authors: Lopes Laise Maia , Ottaiano Gabriel Yoshiaki , de Souza Guedes Luciana , de Moraes Mariana Agostini , Beppu Marisa Masumi 
Published: February 2024

AbstractMucin, a glycoprotein with viscoelastic properties, and silk fibroin, a protein excreted from silkworms with properties of thermal and mechanical resistance, have been probed as building blocks in the design of biomaterials. Aiming to evaluate the interaction and miscibility between mucin and fibroin, we synthesized silk fibroin and mucin (SF/MU) blends for biomedical applications. The morphological analysis of the SF/MU blends showed the presence of two phases, suggesting a partial miscibility between the polymers. The degradation temperature of the SF/MU blends increased with an increase in the silk fibroin content, indicating that silk fibroin contributed to the thermal stability of the blends. The glass transition temperature of the SF/MU blends lay between the values of the pure polymers. The Fourier‐transform infrared spectroscopy results pointed out that the interaction between fibroin and mucin occurred between the amine group of silk fibroin and mucin carboxyl and hydroxyl groups. The outcomes of this work provided essential information on the miscibility of the SF/MU blends. These findings will be critical for further studies with fibroin and mucin‐based biomaterials, especially in mucoadhesive systems and wound healing applications.
Thermally expandable microspheres based on fully or partially bio‐based polymers
Authors: Mousa Maryam , Jonsson Magnus , Granbom Linn , Larsson Kron Anna , Malmström Eva 
Published: February 2024

AbstractBio‐based or partially bio‐based thermally expandable microspheres (TEMs) were synthesized by suspension (co)polymerization of the bio‐based monomer α‐methylene‐γ‐valerolactone (MeMBL) together with acrylonitrile and/or methyl methacrylate to form expandable core/shell particles by encapsulating a hydrocarbon‐based blowing agent. The core/shell polymers were characterized with respect to their chemical structure, thermal expansion, and morphology. The obtained particles, TEMs, showed an increasing onset expansion temperature with increasing content of MeMBL owing to the high glass transition temperature of PMeMBL. As a result, bio‐based/partially bio‐based TEMs are achieved with high thermal stability and expansion properties which can be tailored for various applications.
Thiol‐ene click chemistry for constructing transition layer to enhance the interface interaction between polyacrylonitrile fiber and styrene‐butadiene‐styrene modified asphalt
Authors: Zhang Panpan , Xing Susu , Lin Zibo , Zhao Zhenxia , Kong Hao , Li Jing 
Published: February 2024

AbstractBased on the high chemical stability and excellent end‐group functionalization capability of polyhedral oligomeric silsesquioxane (POSS), this study utilized thiol‐functionalized POSS (POSS‐SH) in a click chemistry reaction with styrene‐butadiene‐styrene triblock copolymer (SBS). The objective was to design a functional group forming a chemical bond with the polymer matrix, resulting in the preparation of thiol‐functionalized polyacrylonitrile (PAN) fibers chemically cross‐linked with POSS‐SH/PAN/SBS hybrid material. This hybrid material not only enhanced the compatibility between the components but also facilitated a connection between the fiber and SBS, thereby improving the mechanical properties of the modified asphalt. Fourier transform infrared spectroscopy, field emission scanning electron microscopy (FESEM), and atomic force microscopy analyses confirmed the successful modification of POSS‐SH/PAN fibers. FESEM analysis of the modified asphalt verified that POSS‐SH/PAN fibers contributed to strengthening the interfacial performance between the fiber and asphalt. Cone penetration and dynamic shear rheometer tests indicated that, compared to SBS‐modified asphalt, PAN/SBS and POSS‐SH/PAN/SBS‐modified asphalt exhibited enhanced shear strength, deformation resistance, and viscoelastic properties. Additionally, at 46°C, the storage modulus (G′) of 1.5% POSS‐SH/PAN/SBS‐modified asphalt increased by 11% compared to 1.5% PAN/SBS asphalt, with increases of 13.75% and 10.8% in loss modulus (G″) and complex modulus (G*), respectively.
Evaluating risks to seabirds on the urban–coastal interface: Modelling dog attacks on little penguin populations in Tasmania
Authors: Blamey Laura K. , Bulman Catherine M. , Tuck Geoffrey N. , Woehler Eric J. , Marker Perviz F. , Patterson Toby A. 
Published: February 2024

Abstract
Tasmania (including offshore islands) has the largest breeding population of little penguins (Eudyptula minor) in Australia, but coastal development around nesting areas has led to habitat loss, often resulting in smaller, fragmented colonies. Colonies have also been subject to predation by introduced domestic species. This study assessed whether historical levels of domestic dog predation could threaten the viability of penguin populations.
Data collected since the 1980s detailing little penguin kills by domestic dogs were used to inform the extent of individual mortality events. The potential impact of dog attacks on the viability of little penguin colonies of the sizes typically found on mainland Tasmania was explored using an age‐based population model. Simulation was conducted over decadal timescales to assess the impact of attacks at varying frequencies and intensities on small (100 birds), medium (300 birds) and large (500 birds) colonies that were stable, growing or decreasing.
Results suggested that frequent and intense dog attacks markedly increased the probability of colony decline, regardless of colony size. Even large, growing penguin colonies were at risk of decline. The risk of extinction was <10% for medium and large stable colonies if no more than 15–25 penguins were killed per attack and there were no more than 20 attacks per 50 years. For small‐sized colonies, the risk of extinction was <10% if fewer than 10 attacks occurred per 50‐year period and no more than 15 birds were killed per attack.
Importantly, results suggested that for small colonies especially, even low levels of predator attack could lead to colony collapse in 10–15 years. For large colonies that were already decreasing, the average time to extinction was markedly increased with additional mortalities from dogs, highlighting the need for ongoing management measures that reduce or eliminate dog attacks to help conserve this iconic species.