bluuebunny/crossref_metadata_2025_split huggingface dataset:
In silico analysis of Bacillus stearothermophilus as a bioindicator for sterilizationco analysis of Bacillus stearothermophilus as a bioindicator for sterilization
Authors: Bushra Nisar Khan  , Nageen Hussain  , Ayesha Aihetasham  , Asia Bibi  , Arshia Mukhtar  , Sadaf Abdullah  , Rana Mohsin Ali  
Published: March 2024

To kill every kind of viable bacterial cells, sterilization in food and pharmaceutical industries is essential. In this study, bio indicators are used to validate accurate sterility. Bacillus stearothermophilus is used as a reference standard heat stable bio indicator. A 16S ribosomal RNA ribotyping sequence of Bacillus stearothermophilus is compared with different bacterial bioindicators including Bacillus pumilus, Bacillus atrophaeus, Clostridium sporogenes, and Brevundimonas diminuta. Results showed that Bacillus pumilus has the highest percentage (90.88%) similarity with the Geobacillus stearothermophilus followed by the Bacillus atrophaeus (90.84%), and Clostridium sporogenes (85.35%). The Brevundimonas diminuta was the least similar organism having a percentage similarity of 81.71% with the Geobacillus stearothermophilus. Bacterial spores are used as biological indicators for sterilization because they exhibit resistance to chemical and physical sterilization techniques. The protein common in all bacteria under study is Spo0A that make the bacteria heat resistant. The eradication of all these kind of heat resistant spores needs a high temperature which will also kill other viable cells.
Keywords: Bacillus stearothermophilus; Bacillus pumilus; Bacillus atrophaeus; Clostridium sporogenes and Brevundimonas diminuta; Spo0A protein.
Antibacterial and anti-biofilm profiling of Syzygium Aro-Maticum Plant Extracts against Multi-drug resistant Human Pathogens
Authors: Fatima Sana , Saba Saba , Hussain Abid , Aslam Laraib , Naveed Azka 
Published: March 2024

Due to rapidly propagating global resistance to antibiotics/pharmacological agents (One Health Challenge) and costly-expenditure for antibiotic formulation, it is primordial to look for efficient alternatives and ample of evidences document the ability of plant extracts in this regard. This study aimed to evaluate antibacterial and anti-biofilm profiles of Syzygium aromaticum extracts against multidrug-resistant (MDR) pathogens. Sampling of blood and urinary catheters was done from clinical laboratory of Multan Pakistan. A total of 06 pathogenic strains: Streptococcus pyogenes, Staphylococcus saprophyticus, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, were isolated and confirmed by 16SrRNA Ribotyping. Kirby-Bauer Disc Diffusion method was employed for resistance profiling. Flowers of S. aromaticum were used to prepare 04 different extracts i.e., Methanolic, Ethanolic, Dimethyl sulfoxide and Aqueous. Phytochemical screening showed all extracts positive for Proteins, Carbohydrates, Glycosides, Terpenoids, Saponins and Steroids. MIC and MBC value of all extracts was found 40 µg/ml and 160 µg/ml respectively except Methanolic extract (20 µg/ml and 80 µg/ml respectively). Highest anti-biofilm activity was seen for methanolic extracts. FRAP % was found directly proportional to extract concentration while DDPH % inhibition was: methanolic > ethanolic >Aqueous > DMSO extract. In TLC Analysis, highest Rf value was recorded for Methanolic extract. FTIR analysis showed different peaks as: Alkyl halide-698.58/cm, Aromatic group-879/cm, Alcohol-1013.63/cm, Polysaccharides-1045.10/cm, Carboxylic acid-1436.15/cm, Amides-1515.01/cm and Aldehydes/ketones-2975.35/cm. It is concluded that Methanolic extract of Syzygium aromaticum was found to have highest antibacterial, anti-biofilm and antioxidant potential thus its secondary metabolites are cost-effective candidates which can fuel the future pipeline for antibiotic discovery.
Phyto-extracts Mediated Biological Control of Citrus Canker
Authors: Muhammad Usama Bin Tariq  , Riaz Hasan , Muhammad Arslan Khan  , Mirza Abdul Qayyum  , Sami Ullah  , Muhammad Hassan  , Farrukh Shahzad  , Muhammad Shan Latif  
Published: March 2024

Citrus is second most important fruit crop and has significance economic importance in Pakistan. In Pakistan 2.17 million tonnes citrus was produced on area of 1.94 million acre. Citrus is mostly cultivated in Punjab and affected by different types of plant pathogens. Citrus canker is the most harmful disease among the major diseases of citrus which is caused by Xanthomonas axonopodis pv. citri. The copper based fungicides are mostly employed to manage the citrus canker in the field but excessive use of chemicals have negatively impacted the environment and warrant the need to find out organic solutions therefore the current study was aimed at identifying suitable plants extracts for the control of citrus canker bacterium. Isolation of the bacterium X.axonopodis was done through streaking on agar plate technique. Morphological and bio-chemical tests such as gram staining, KoH test, catalase test and starch hydrolysis test were performed to examine the bacteria X.axonopodispv. citri. Plant extracts were evaluated, in vitro, against the X.axonopodis pv. citri through paper disc method which showed the inhibition zone against the disease. The 100 percent extract of garlic, ginger, aloevera, moringa were used used along with the streptomycin as a positive control. The inhibition zone was 11.67mm and 10.83mm due to garlic and moringa extracts respectively while it was 16.67mm in case of positive control. The ginger and aloevera extracts proved ineffective against the citrus canker bacterium.The efficacy of garlic and moringa extracts were also checked against the X. axonopodis pv. citri under glasshouse conditions. The inoculum was sprayed on Kinnow plants, with three replicates, for each extract and data was recorded 15, 20, 25, 30 and 35 days post inoculation (dpi).The recorded disease severity percentage, after 35dpi, on garlic and moringa treated plants were 9.67% and 14.27% respectively while the 27% disease severity was recorded on control (plants sprayed with autoclaved distilled water). The garlic extract proved to be most effective followed by moringa extract which could be used as a potential organic solution to control citrus canker.
Hopeless Waiting and Apprehension of Missing Out
Authors: Alrawi Ahmed 
Published: March 2024

Abstract
This research examines the intersection between the policy issues and the experiences Iraqis have with cellular broadband connections (CBCs) in rural Iraq. Findings imply that the Iraqi rural broadband policy has failed in the following areas: subsidy, licenses, and tax relief. These failures happen due to favoring private cellular broadband providers (CBPs) over rural public interest. Further, the policy failure affected rural Iraqis in the following aspects: social seclusion, apprehension of missing out, waiting without hope, and limited alternatives. This research brings new information about the Iraqi telecom sector that could contribute to filling the knowledge gap in this field.
La tuberculose et ses aspects épidémio-cliniques et évolutifs au service de Médecine Interne de l’Hôpital National Donka (Guinée)
Authors: Wann TA , Al Et 
Published: March 2024

Introduction : La tuberculose est une maladie infectieuse à transmission interhumaine liée au bacille de koch (BK). Elle est l’une des pathologies infectieuses causant le plus de décès dans le monde. Notre objectif était de déterminer les aspects épidémiologiques, cliniques et évolutifs de la tuberculose au service de Médecine Interne de l’Hôpital National Donka.
Matériel et Méthodes : Il s’agissait d’une étude rétrospective de type descriptif portant sur les dossiers médicaux des patients au service de médecine interne (SMI) de l’hôpital national Donka sur une période d’étude allant de janvier 2017 à juin 2023. Nous avons inclus, tous les dossiers des patients âgés de plus de 15 ans durant la période d’étude et présentant les signes d’imprégnation tuberculeuse. Résultats : Durant la période d’étude 145 patients avec 81 (55,86 %) hommes et 64 (44,14 %) femmes ont été colligés. L’âge moyen de nos patients était de 44 ans avec une prédominance masculine soit un sex-ratio de 1,26. Soixante-quinze patients (51,72 %) étaient des patients infectés par le virus de l’immunodéficience humaine (VIH). La tuberculose était de localisation multifocale dans 69 (47,59 %) des cas, neuro-méningée chez 31 (21,38 %) des cas et pulmonaire dans 45 (31,03 %) des cas. La symptomatologie clinique était dominée par l’altération de l’état général et la fièvre chez tous nos patients, les vomissements chez 61 (42,07 %) patients. Les signes radiologiques les plus fréquents étaient les nodules pulmonaires (53,36 %) et les adénopathies profondes (36,42%). La tuberculose était confirmée par gène expert dans 38,27% des cas. L’intradermo-réaction à la tuberculine était positive dans 42 % des cas. L’évolution était favorable chez 68 % des patients, vers une aggravation dans 32 % des cas avec un taux de décès de 26%. Conclusion : La tuberculose demeure encore un problème de santé publique dans nos régions surtout depuis l’avènement du VIH/Sida. Cette affection concerne surtout les hommes dans notre contexte.
Medical students’ experiences and perspectives on simulation-based education
Authors: Ensor Nicholas , Sivasubramaniam Mithila , Laird Ashleigh J. , Roddis Bridget , Qin Kirby R. , Pacilli Maurizio , Nestel Debra , Nataraja Ramesh M. 
Published: March 2024

We aimed to gain insight into student experiences of simulation-based education (SBE), particularly in regards to (1) current provision of SBE, (2) learning opportunities and (3) areas for improvements, innovation and focused initiatives. This was to create focussed initiatives that can assess and address specific needs to improve SBE for learners and educators alike.
Medical students were surveyed with 41 questions on their experiences of SBE using an 11-point Likert scale (0 – strongly disagree, 10 – strongly agree). Results were analysed by individual questions and presented as median (interquartile range) or percentage (
246 students participated, with 76.0% (187/246) completing all questions. 99.2% of students (235/237) had participated in SBE. The most valuable elements of SBE were learning a new skill under supervision (90.3%, 187/207), applying prior knowledge to a clinical scenario (73.4%, 152/187) and identifying gaps in knowledge/skill (73.4%, 152/187). Simulation was thought to improve medical knowledge (95.2%, 218/229) and technical skills (87.3%, 200/229). Twenty-one per cent (41/197) of students reported a negative experience and 23.5% (48/204) felt anxious. Students strongly agreed that simulation was beneficial to their training (9 [8–10]) and that there should be more SBE (8.5 [8–10]).
Medical students find SBE accessible and valuable to their education. By analysing student perspectives (such as self-reported negative experience), targeted areas for further research and focussed initiatives can be implemented.
CARACTERIZAÇÃO MORFOMÉTRICA DAS MICROBACIAS ÁGUA DO JAÚ E ÁGUA DA EMA, MUNICÍPIO DE ROLÂNDIA – PR
Authors: Ribeiro Fernanda Leite , Nardini Rafael Calore , Silva Maria Carolina da , Lima Rosana Kostecki de , Vendrame Pedro Rodolfo Siqueira , Gomes Luciano Nardini , Oliveira Guilherme , Pereira Neto Osvaldo Coelho 
Published: March 2024

A Caracterização morfométrica auxilia o estudo do comportamento da bacia hidrográfica, principalmente no aspecto envolvendo riscos de processo erosivo relacionado a enchentes. O presente estudo teve como objetivo caracterizar a morfometria das microbacias Água do Jaú e Água da Ema, localizadas no município de Rolândia – PR, utilizando software AutoCad como ferramenta de digitalização e quantificação de canais e carta planialtimétrica folha de Arapongas como base cartográfica. Encontram-se entre as seguintes coordenadas UTM: 7414000 a 7428000 Km N e 448000 a 456000 Km E, com uma área total de 5.081 ha. Os resultados demonstram microbacias de 2a ordem de ramificação de canais e baixa densidade de drenagem. O índice de conformação, fator de forma e o coeficiente de compacidade indicam formato alongado. O índice de sinuosidade indica tendência a canais retilíneos. A declividade média das microbacias classificou o relevo como ondulado. Os parâmetros dimensionais juntamente com os índices morfométricos relacionados a forma, relevo e de composição e padrão da rede de drenagem permitiram inferir que as áreas de estudo apresentam baixa susceptibilidade a enchentes e riscos de erosões, refletindo tendência a conservação dos solos e dos recursos hídricos.
ESCUELAS RURALES Y COLONIZACIÓN EN EL PERIURBANO BONAERENSE, UN ESTUDIO DE CASO (1946-1955)
Authors: De Marco Rosa María Celeste 
Published: March 2024

El presente trabajo se propone analizar la relación existente entre educación y colonización agrícola durante el gobierno del peronismo histórico (1946-1955) en el ámbito periurbano de la provincia de Buenos Aires (Argentina) considerando los cambios y continuidades con el período precedente. Esta vinculación será abordada en el marco de las políticas educativas de las gobernaciones de Domingo Mercante (1946-1952) y Carlos Aloé (1952-1955) en cuanto a educación rural, articulándola asimismo con el plan de colonización desarrollado en la provincia. Con ese fin, se caracterizará la política del Instituto Autárquico de Colonización, los aspectos institucionales y normativos que condujeron a la instalación de ofertas educativas en el medio rural, en el marco de un discurso que valoraba la vida en el campo, pero asimismo el abastecimiento de alimentos a las ciudades. Nos enfocaremos, como estudio de caso en la colonia agrícola “17 de Octubre”, ubicada en la zona rural del partido de Florencio Varela (Buenos Aires) y la refundación de una unidad educativa vinculada a la misma, la escuela primaria rural Nº 4 “Florencio Varela”.
ATRIAL FIBRILLATION IN PATIENTS WITH CANCER (ANALYSIS OF LITERARY SOURCES)
Authors: Федорец В.Н. , Вологдина И.В. , Василенко В.С. , Порошина Е.Г. , Красильникова Л.А. 
Published: March 2024

Фибрилляция предсердий (ФП) является одним из наиболее часто встречающихся и прогностически неблагоприятных осложнений заболеваний сердечно-сосудистой системы. С помощью баз данных Elibrary.ru, PubMed, Scopus и сетевого приложения ResearchGate был проведен всесторонний поиск публикаций, анализирующих взаимосвязь фибрилляции предсердий и онкологических заболеваний. В обзоре литературы рассмотрено 45 публикаций о распространенности ФП у онкологических больных, влиянии противоопухолевых препаратов и других методов лечения на данную группу пациентов. В число подобных статей входят клинические рекомендации, согласованные мнения экспертов и ранее опубликованные обзоры литературы. Рекомендации по менеджменту фибрилляции предсердий у пациентов, получающих противоопухолевое лечение, изложены с позиции уровней и классов доказательности Рекомендаций по кардиоонкологии Европейского общества кардиологов (2022).
Atrial fibrillation is one of the most common and prognostically unfavorable complications of the cardiovascular system diseases. А comprehensive search was conducted using the databases Elibrary ru, PubMed, Scopus and the ResearchGate network application for analyzing the relationship of atrial fibrillation and oncological diseases. The literature review reviewed 45 publications on the prevalence of AF in cancer patients, the effect of antitumor drugs and other treatment methods on this group of patients. Analyzed articles include clinical recommendations, consensus expert opinions, and previously published literature reviews. Recommendations for the management of atrial fibrillation in patients receiving anticancer treatment are presented from the perspective of the levels and classes of evidence of the 2022 ESC Guidelines on cardio-oncology.
MODELO DE SUSTENTABILIDAD Y GESTIÓN DE PARQUES URBANOS: Herramientas para la construcción de un tool-kit en base a un estudio de referentes
Authors: Arizaga Ximena , Moreno Osvaldo , Tapia Josefina 
Published: March 2024

The National Urban Parks Policy (2021) seeks the development of green areas of more than 2 hectares in all the cities of Chile, while recognizing that the management and maintenance of urban parks is one of the main challenges. In order to define a methodology for improving the management and operation of parks and green areas, a case study was carried out aimed at identifying good international practices in sustainability models and management of Urban Parks. Based on this analysis, the methodology defines an "ideal model" and seeks to understand how gaps could be solved and efficient management achieved through the systematization of a catalog: institution, administration, financing and uses. This, together with the characterization of community needs and capacities, makes it possible to define a Tool-kit of instruments for the Sustainability and management of Urban Parks adapted to different municipal’s realities and scales of parks.

Keywords: Urban Parks, Sustainability, Green Infrastructure, Management
La Política Nacional de Parques Urbanos (2021) busca el desarrollo de áreas verdes de más de 2 hectáreas en todas las ciudades de Chile a la vez que reconoce como uno de los principales desafíos la gestión y mantención de los parques urbanos. Con objeto de definir una metodología para el mejoramiento de la gestión y operación de los parques y áreas verdes, se realizó un estudio de casos orientado a identificar las buenas prácticas internacionales en modelos de sustentabilidad y gestión de Parques Urbanos. Sobre esta base, la metodología propone la definición de un “modelo ideal”, y busca comprender cómo se podrían solucionar las brechas para alcanzar una gestión eficiente, a través de la sistematización de las materias abordadas en el catálogo: institucionalidad, administración, financiamiento y usos. Lo anterior, junto con la caracterización de las necesidades y capacidades comunales permite definir un Tool-kit de instrumentos para la sustentabilidad y gestión de Parques Urbanos adaptable a distintas realidades y escalas de parques.

Palabras clave: Parques Urbanos, Sustentabilidad, Infraestructura Verde, Gestión.
USO E APROPRIAÇÃO DO ESPAÇO PÚBLICO: Um estudo de caso na Praça Santos Dumont em Florianópolis (SC)
Authors: Lima Felisberto Lara , Francisco Reis Almir 
Published: March 2024

Public spaces play an important role in promoting social interactions between different social agents. In thiscontext, it is important to evaluate appropriation in public spaces and identify the different forms that generatediversity in them. Santos Dumont Square, the empirical object of this work, corresponds to a centrality of theTrindade neighborhood in Florianópolis (SC) and is characterized by the diversity of uses in the surroundings.The objective of the research is to carry out an analysis of the appropriation of this space, quantifying andanalyzing users and predominant activities. For this, the reading of co-presence (Gehl & Svarre, 2018;Tenório, 2012) and the behavioral map (Sommer & Sommer, 1997) were used as a methodology. The resultsshow that flows are intense on the periphery of the square and are concentrated during business hours.These results reflect the difficulties posed by the existing unevenness and the predominance of commercialuses and services in the immediate surroundings.
Keywords: appropriation, public space, squares, copresence
Os espaços públicos desempenham um importante papel na promoção de interações entre diferentesagentes sociais. Neste contexto, surge a importância de avaliar a apropriação nos espaços públicos eidentificar as diferentes formas geradoras de diversidade no mesmo. A Praça Santos Dumont, objetoempírico deste trabalho, corresponde a uma centralidade do bairro Trindade em Florianópolis (SC) e secaracteriza pela diversidade de usos no entorno. O objetivo da pesquisa é realizar uma análise daapropriação desse espaço quantificando e analisando usuários e atividades predominantes. Para isso, usousecomo metodologia a leitura da copresença (Gehl & Svarre, 2018; Tenório, 2012) e o mapacomportamental (Sommer & Sommer, 1997). Os resultados mostram que os fluxos são intensos na periferiada praça e se concentram nos horários comerciais. Estes resultados refletem as dificuldades colocadas pelosdesníveis existentes e a predominância de usos comerciais e de serviços no entorno imediato.
Palavras-chave: apropriação, espaço público, praças, copresença
UM NOVO OLHAR SOBRE A ROSA MÍSTICA. Proposta de requalificação urbana e ambiental de trecho do Riacho da Piabas.
Authors: Ramos Tejo Bruna , Normando Macêdo Barros Filho Mauro 
Published: March 2024

The objective of this work is to present a proposal for the urban and environmental redevelopment of a section of Riacho das Piabas, located within the Rosa Mística community in Campina Grande. The methodology employed various procedures: (i) characterization of the integration of the Permanent Preservation Area within the Open Spaces System; (ii) qualitative analysis of previously conducted urban assessments; and (iii) utilization of the SWOT matrix as a strategy for formulating guidelines and actions. The outcome is the design of a linear park, accompanied by two open spaces, with guidelines aimed at mitigating floods, restoring thecreek's ecological balance, and fostering strategies to enhance the city's response to crisis situations. Ultimately, this work is expected to contribute to the promotion of environmental justice and the establishment of more equitable and sustainable societies.
Keywords: Urban requalification. Urban design. Urban sustainability. Open spaces.
O trabalho tem como objetivo apresentar uma proposta para a requalificação urbana e ambiental de um trecho do Riacho das Piabas que está inserido na comunidade Rosa Mística, em Campina Grande. A metodologia se deu por diferentes procedimentos: (i) caracterização da integração da Área de Preservação Permanente no contexto do Sistema de Espaços Livres; (ii) análise qualitativa dos diagnósticos urbanísticos previamente levantados; e (iii) utilização da matriz SWOT como estratégia de desenvolvimento de diretrizes e ações. O resultado foi o projeto de um parque linear integrado a dois espaços livres, cujas diretrizes buscam a delimitação de meios de mitigação de enchentes, a despoluição e renaturalização do riacho, assim como a promoção de estratégias para a consolidação de melhores respostas urbanas às situações de crise. Por fim, espera-se que este trabalho contribua para a promoção da justiça ambiental e para a construção de sociedades mais equitativas e sustentáveis.

Palavras-chave: Requalificação urbana. Projeto urbano. Sustentabilidade urbana. Espaços livres