bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of robotic arm for the porcelain bushing in substation
Authors: Chen Hao , Han Wei , Xu Weilun , Tang Zongyao , Chen Yini , Xu Peng , Ma Zhaoxing 
Published: April 2024

AbstractWith the development and the application popularization of artificial intelligence robot technology and 5G technology, a robotic arm is designed and developed for rinsing porcelain bushing in high voltage substation in this paper. Firstly, the components and implementation of robotic arm are presented, subsequently, a circular cleaning structure with a 120-degree split is proposed to rinse the porcelain bushing. Secondly, a two-stage simple and effective method to realize automatic orientation is proposed utilizing photoelectric switches. Moreover, a prototype of robotic arm with control system is developed based on the regime switching function, and the result of edge computing is transmitted by 5G technology. Finally, feasibility and effectiveness of the robotic arm are verified in the Nanjing power grid. The case study manifests that the robotic arm developed by the proposed method in the paper can achieve efficient rinsing and all the corresponding information can be transmitted preciously. The proposed method lays a foundation for wide application of cleaning robot in high voltage substation.
The angle of arrival estimation of frequency-hopping cooperative object based on software-defined radio
Authors: Rustamaji  , Kliwati Sri , Widada Wahyu 
Published: April 2024

AbstractThe primary objective of this research is to propose and examine a technique for accurately determining the azimuth angle of cooperative objects. The proposed methodology aims were to achieve fast processing, increased precision, and enhanced safety. A transmitter emitting a continuous wave with a hopping frequency was utilized in combination with interferometry techniques to measure the angle of arrival (AOA) between two antennas. The efficient method incorporates a coarse-to-fine strategy that improves processing speed and azimuth angle accuracy and to effectively eliminate any signal interference, a Fourier bandpass filter is utilized. The coarse estimation is performed using the fast Fourier transform (FFT) algorithm, while the fine estimation is achieved using the multiple signal classification (MUSIC) algorithm. The fine estimation involves utilizing coarse angle input and a scan limit of 2.5° that is determined from the largest simulated root mean square error (RMSE) value. Modelling and outdoor testing using software defined radio (SDR) have been carried out to assess the proposed methodology. The results from the analysis indicate that the proposed method produces the desired RMSE estimation of less than 1°, thereby validating its accuracy and effectiveness.
Ocular blood flow in preterm neonates
Authors: Silverman Ronald H. , Urs Raksha , Horowitz Jason D. , Coki Osode , Pinto Leora 
Published: April 2024

AbstractRetinopathy of prematurity (ROP) is a disorder affecting low birthweight, preterm neonates. In the preterm eye, the retina is not fully developed and neovascularization may occur at the margin between the developed vascular retina and undeveloped avascular retina. Without timely treatment by laser or intravitreal anti-vascular endothelial growth factor (VEGF) therapy, this can lead to tractional retinal detachment and blindness. Visualization of the retina in regular examinations by indirect ophthalmoscopy is hence the current standard of care, but the exams are stressful and interpretation of images is subjective. The upregulation of VEGF in ROP would suggest an increase in ocular blood flow. In this report, we evaluate the potential of ultrafast plane-wave Doppler ultrasound (PWU) to detect increased flow velocities in the orbital vessels supplying the eye in a gentle exam with objective findings. We imaged both eyes of 50 low-birthweight preterm neonates using 18 MHz PWU. Flow velocity in the central retinal artery (CRA) and vein (CRV), and the short posterior ciliary arteries were determined and values at each ROP Stage compared. We found significantly increased velocities in the CRA and CRV in Stage 3 ROP eyes, where intervention would be considered. We compared multivariate models for identifying Stage 3 eyes comprised solely of clinical factors, solely of Doppler parameters, and clinical plus Doppler parameters. The respective models provided areas under their respective ROC curves of 0.760, 0.812, and 0.904. PWU Doppler represents a gentle, objective means for identifying neonates at risk for ROP that could complement ophthalmoscopy.
Screening of cellulose-degrading bacteria and optimization of cellulase production from Bacillus cereus A49 through response surface methodology
Authors: Wang Jinjun , Bao Fei , Wei Huixian , Zhang Yang 
Published: April 2024

AbstractCellulose-degrading microorganisms hold immense significance in utilizing cellulose resources efficiently. The screening of natural cellulase bacteria and the optimization of fermentation conditions are the hot spots of research. This study meticulously screened cellulose-degrading bacteria from mixed soil samples adopting a multi-step approach, encompassing preliminary culture medium screening, Congo red medium-based re-screening, and quantification of cellulase activity across various strains. Particularly, three robust cellulase-producing strains were identified: A24 (MT740356.1 Brevibacillus borstelensis), A49 (MT740358.1 Bacillus cereus), and A61 (MT740357.1 Paenibacillus sp.). For subsequent cultivation experiments, the growth curves of the three obtained isolates were monitored diligently. Additionally, optimal CMCase production conditions were determined, keeping CMCase activity as a key metric, through a series of single-factor experiments: agitation speed, cultivation temperature, unit medium concentration, and inoculum volume. Maximum CMCase production was observed at 150 rpm/37 °C, doubling the unit medium addition, and a 5 mL inoculation volume. Further optimization was conducted using the selected isolate A49 employing response surface methodology. The software model recommended a 2.21fold unit medium addition, 36.11 °C temperature, and 4.91 mL inoculant volume for optimal CMCase production. Consequently, three parallel experiments were conducted based on predicted conditions consistently yielding an average CMCase production activity of 15.63 U/mL, closely aligning with the predicted value of 16.41 U/mL. These findings validated the reliability of the model and demonstrated the effectiveness of optimized CMCase production conditions for isolate A49.
Fecal DNA analysis coupled with the sighting records re-expanded a known distribution of dugongs in Ryukyu Islands after half a century
Authors: Ozawa Hiroyuki , Yoshihama Takahiro , Gishitomi Shogo , Watanabe Natsuki , Ichikawa Kotaro , Sato Keiichi , Watanabe Kenta , Takano Katsuhiko , Ochiai Yosuke , Yamanaka Hiroki , Maruyama Atsushi 
Published: April 2024

AbstractDNA analysis of large herbivore feces samples collected from seagrass beds at two distant sites (Irabu Island in Miyako Islands and Kushi in Okinawa Island) in the Ryukyu Islands proved that some of these feces were from dugongs, which had been treated in recent studies as extinct in this region since the last stranding of a deceased individual in 2019. In addition, local knowledge of sightings of animals thought to be dugongs and confirmed cases of dugong feeding trails since 2010 were compiled to estimate its recent distribution. This is the first scientific report on the presence of this mammal in the Ryukyu Islands within the last four years, and particularly in the Miyako Islands within the last half-century. As the Ryukyu Islands are known to be the northern limit of the dugong’s fragmented distribution in East Asia, conservation efforts are therefore needed.
q-Diffusion leverages the full dimensionality of gene coexpression in single-cell transcriptomics
Authors: Marmarelis Myrl G. , Littman Russell , Battaglin Francesca , Niedzwiecki Donna , Venook Alan , Ambite Jose-Luis , Galstyan Aram , Lenz Heinz-Josef , Ver Steeg Greg 
Published: April 2024

AbstractUnlocking the full dimensionality of single-cell RNA sequencing data (scRNAseq) is the next frontier to a richer, fuller understanding of cell biology. We introduce q-diffusion, a framework for capturing the coexpression structure of an entire library of genes, improving on state-of-the-art analysis tools. The method is demonstrated via three case studies. In the first, q-diffusion helps gain statistical significance for differential effects on patient outcomes when analyzing the CALGB/SWOG 80405 randomized phase III clinical trial, suggesting precision guidance for the treatment of metastatic colorectal cancer. Secondly, q-diffusion is benchmarked against existing scRNAseq classification methods using an in vitro PBMC dataset, in which the proposed method discriminates IFN-γ stimulation more accurately. The same case study demonstrates improvements in unsupervised cell clustering with the recent Tabula Sapiens human atlas. Finally, a local distributional segmentation approach for spatial scRNAseq, driven by q-diffusion, yields interpretable structures of human cortical tissue.
A spatiotemporal proteomic map of human adipogenesis
Authors: Klingelhuber Felix , Frendo-Cumbo Scott , Omar-Hmeadi Muhmmad , Massier Lucas , Kakimoto Pamela , Taylor Austin J. , Couchet Morgane , Ribicic Sara , Wabitsch Martin , Messias Ana C. , Iuso Arcangela , Müller Timo D. , Rydén Mikael , Mejhert Niklas , Krahmer Natalie 
Published: April 2024

AbstractWhite adipocytes function as major energy reservoirs in humans by storing substantial amounts of triglycerides, and their dysfunction is associated with metabolic disorders; however, the mechanisms underlying cellular specialization during adipogenesis remain unknown. Here, we generate a spatiotemporal proteomic atlas of human adipogenesis, which elucidates cellular remodelling as well as the spatial reorganization of metabolic pathways to optimize cells for lipid accumulation and highlights the coordinated regulation of protein localization and abundance during adipocyte formation. We identify compartment-specific regulation of protein levels and localization changes of metabolic enzymes to reprogramme branched-chain amino acids and one-carbon metabolism to provide building blocks and reduction equivalents. Additionally, we identify C19orf12 as a differentiation-induced adipocyte lipid droplet protein that interacts with the translocase of the outer membrane complex of lipid droplet-associated mitochondria and regulates adipocyte lipid storage by determining the capacity of mitochondria to metabolize fatty acids. Overall, our study provides a comprehensive resource for understanding human adipogenesis and for future discoveries in the field.
Direct and selective pharmacological disruption of the YAP–TEAD interface by IAG933 inhibits Hippo-dependent and RAS–MAPK-altered cancers
Authors: Chapeau Emilie A. , Sansregret Laurent , Galli Giorgio G. , Chène Patrick , Wartmann Markus , Mourikis Thanos P. , Jaaks Patricia , Baltschukat Sabrina , Barbosa Ines A. M. , Bauer Daniel , Brachmann Saskia M. , Delaunay Clara , Estadieu Claire , Faris Jason E. , Furet Pascal , Harlfinger Stefanie , Hueber Andreas , Jiménez Núñez Eloísa , Kodack David P. , Mandon Emeline , Martin Typhaine , Mesrouze Yannick , Romanet Vincent , Scheufler Clemens , Sellner Holger , Stamm Christelle , Sterker Dario , Tordella Luca , Hofmann Francesco , Soldermann Nicolas , Schmelzle Tobias 
Published: April 2024

AbstractThe YAP–TEAD protein–protein interaction mediates YAP oncogenic functions downstream of the Hippo pathway. To date, available YAP–TEAD pharmacologic agents bind into the lipid pocket of TEAD, targeting the interaction indirectly via allosteric changes. However, the consequences of a direct pharmacological disruption of the interface between YAP and TEADs remain largely unexplored. Here, we present IAG933 and its analogs as potent first-in-class and selective disruptors of the YAP–TEAD protein–protein interaction with suitable properties to enter clinical trials. Pharmacologic abrogation of the interaction with all four TEAD paralogs resulted in YAP eviction from chromatin and reduced Hippo-mediated transcription and induction of cell death. In vivo, deep tumor regression was observed in Hippo-driven mesothelioma xenografts at tolerated doses in animal models as well as in Hippo-altered cancer models outside mesothelioma. Importantly this also extended to larger tumor indications, such as lung, pancreatic and colorectal cancer, in combination with RTK, KRAS-mutant selective and MAPK inhibitors, leading to more efficacious and durable responses. Clinical evaluation of IAG933 is underway.
Sustained ocean cooling insufficient to reverse sea level rise from Antarctica
Authors: Alevropoulos-Borrill Alanna , Golledge Nicholas R. , Cornford Stephen L. , Lowry Daniel P. , Krapp Mario 
Published: April 2024

AbstractGlobal mean sea level has risen at an accelerating rate in the last decade and will continue to rise for centuries. The Amundsen Sea Embayment in West Antarctica is a critical region for present and future ice loss, however most studies consider only a worst-case future for the region. Here we use ice sheet model sensitivity experiments to investigate the centennial scale implications of short-term periods of enhanced ocean driven sub-ice shelf melting on ice loss and assess what future reduction in melting is necessary to mitigate ice stream retreat and offset global sea level rise. Our findings reveal that restoring elevated melt rates to present-day levels within 100 years causes rates of ice discharge to immediately decline, thereby limiting the overall sea level contribution from the region. However, while ice stream re-advance and slowed ice discharge is possible with reduced basal melting, a centennial scale increase in accumulation must occur to offset the extensive ice loss.
Microbial communities modulate chemical weathering and carbon dioxide cycling in an active orogen in Taiwan
Authors: Wang Pei-Ling , Tu Tzu-Hsuan , Lin Li-Hung , Chou Hsi-Ling , Wang Yi-Jie , Chen Jhen-Nien , Wang Lu-Yu , Chang Jui-Ming , Chu Mei-Fei , Hsu Yi-Chun , Chang Chung-Pai , Wu Yih-Ming , Lin Yen-Tsu , Ke Chien-Chung 
Published: April 2024

AbstractChemical weathering modulates carbon transfer between the crust, hydrosphere, and atmosphere. The extent to which microbial processes are involved in mineral dissolution remains elusive. Here, we performed geochemical and molecular analyses of river water and other materials collected from a rapidly exhuming catchment in eastern Taiwan. In addition to solute generation driven primarily by pyrite-induced carbonate weathering, highly skewed microbial community compositions with abundant Sulfuricurvum and Thiobacillus members were detected during high-water periods. The yields of these taxa were also correlated with those of sulfate and sediments, suggesting that pyrite oxidation and carbonate dissolution were facilitated by sulfur-respiring microorganisms inhabiting erodible materials at a pace comparable to the supply of sulfur-bearing minerals through rapid exhumation. The net CO2 export regulated by such potentially supply-limited, microbially-mediated mineral weathering greatly surpasses the global average, highlighting active orogens in high-standing islands as important CO2 contributors rendered by tandem biotic and abiotic processes.
Nuclear phosphoinositide signaling promotes YAP/TAZ-TEAD transcriptional activity in breast cancer
Authors: Jung Oisun , Baek Min-jeong , Wooldrik Colin , Johnson Keith R , Fisher Kurt W , Lou Jinchao , Ricks Tanei J , Wen Tianmu , Best Michael D , Cryns Vincent L , Anderson Richard A , Choi Suyong 
Published: April 2024

AbstractThe Hippo pathway effectors Yes-associated protein 1 (YAP) and its homolog TAZ are transcriptional coactivators that control gene expression by binding to TEA domain (TEAD) family transcription factors. The YAP/TAZ–TEAD complex is a key regulator of cancer-specific transcriptional programs, which promote tumor progression in diverse types of cancer, including breast cancer. Despite intensive efforts, the YAP/TAZ–TEAD complex in cancer has remained largely undruggable due to an incomplete mechanistic understanding. Here, we report that nuclear phosphoinositides function as cofactors that mediate the binding of YAP/TAZ to TEADs. The enzymatic products of phosphoinositide kinases PIPKIα and IPMK, including phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (P(I3,4,5)P3), bridge the binding of YAP/TAZ to TEAD. Inhibiting these kinases or the association of YAP/TAZ with PI(4,5)P2 and PI(3,4,5)P3 attenuates YAP/TAZ interaction with the TEADs, the expression of YAP/TAZ target genes, and breast cancer cell motility. Although we could not conclusively exclude the possibility that other enzymatic products of IPMK such as inositol phosphates play a role in the mechanism, our results point to a previously unrecognized role of nuclear phosphoinositide signaling in control of YAP/TAZ activity and implicate this pathway as a potential therapeutic target in YAP/TAZ-driven breast cancer.
Lacticaseibacillus rhamnosus HF01 fermented yogurt alleviated high-fat diet-induced obesity and hepatic steatosis via the gut microbiota–butyric acid–hepatic lipid metabolism axis
Authors: Sun Yue , Liu Xiaolin , Wang Rongchun , Liu Rongmei , Lv Xuepeng , Ma Ying , Li Qiming 
Published: April 2024

The fermented yogurt of Lacticaseibacillus rhamnosus HF01 alters obese mice gut microbiota by boosting beneficial flora and butyric acid, activates AMPK to regulate lipid metabolism, combating obesity.
A water mediated multicomponent reaction for the synthesis of novel spirooxindole derivatives and their antifungal activity
Authors: Zhang Min , Shi Liang , Chen Li , Liu Zhengyu , Zhao Ting , Zhu Chunyin , Yang Liuqing 
Published: April 2024

A total of 27 novel spirooxindoles were synthesized through a water mediated three-component reaction of isatin, 4-aminocoumarin, and 1,3-cyclodicarbonyl compounds. Several compounds showed excellent antifungal activity against Physalospora piricola.
Synergistic action of synthetic peptides and amphotericin B causes disruption of the plasma membrane and cell wall in Candida albicans
Authors: Souza Thayna A.M. , Mello Erica O. , Taveira Gabriel B. , Moreira Felipe F. , Seabra Sergio Henrique , Carvalho André O. , Gomes Valdirene M. 
Published: April 2024

Abstract
The objective of this work was to evaluate the combination of synthetic peptides based on the γ-core motif of defensin PvD1 with amphotericin B (AmB) at different concentrations against Candida albicans. We applied the checkerboard assay using different concentrations of the commercial drug AmB and the synthetic peptides γ31-45PvD1++ and γ33-41PvD1++ against C. albicans, aiming to find combinations with synergistic interactions. Between these two interactions involving γ31-45PvD1++ and AmB, an additive effect was observed. One such interaction occurred at concentrations of 0.009 µM of peptide γ31-45PvD1++ and 13.23 µM of AmB and another condition of 0.019 µM of peptide γ31-45PvD1++ and 6.61 µM of AmB. The other two concentrations of the interaction showed a synergistic effect in the combination of synthetic peptide γ31-45PvD1++ and AmB, where the concentrations were 1.40 µM peptide γ31-45PvD1++ and 0.004 µM AmB and 0.70 µM γ31-45PvD1++ peptide and 0.002 µM AmB. We proceeded with analysis of the mechanism of action involving synergistic effects. This examination unveiled a range of impactful outcomes, including the impairment of mitochondrial functionality, compromise of cell wall integrity, DNA degradation, and a consequential decline in cell viability. We also observed that both synergistic combinations were capable of causing damage to the plasma membrane and cell wall, causing leakage of intracellular components. This discovery demonstrates for the first time that the synergistic combinations found between the synthetic peptide γ31-45PvD1++ and AmB have an antifungal effect against C. albicans, acting on the integrity of the plasma membrane and cell wall.
3D shape knowledge graph for cross‐domain 3D shape retrieval
Authors: Chang Rihao , Ma Yongtao , Hao Tong , Wang Weijie , Nie Weizhi 
Published: April 2024

AbstractThe surge in 3D modelling has led to a pronounced research emphasis on the field of 3D shape retrieval. Numerous contemporary approaches have been put forth to tackle this intricate challenge. Nevertheless, effectively addressing the intricacies of cross‐modal 3D shape retrieval remains a formidable undertaking, owing to inherent modality‐based disparities. The authors present an innovative notion—termed “geometric words”—which functions as elemental constituents for representing entities through combinations. To establish the knowledge graph, the authors employ geometric words as nodes, connecting them via shape categories and geometry attributes. Subsequently, a unique graph embedding method for knowledge acquisition is devised. Finally, an effective similarity measure is introduced for retrieval purposes. Importantly, each 3D or 2D entity can anchor its geometric terms within the knowledge graph, thereby serving as a link between cross‐domain data. As a result, the authors’ approach facilitates multiple cross‐domain 3D shape retrieval tasks. The authors evaluate the proposed method's performance on the ModelNet40 and ShapeNetCore55 datasets, encompassing scenarios related to 3D shape retrieval and cross‐domain retrieval. Furthermore, the authors employ the established cross‐modal dataset (MI3DOR) to assess cross‐modal 3D shape retrieval. The resulting experimental outcomes, in conjunction with comparisons against state‐of‐the‐art techniques, clearly highlight the superiority of our approach.
An optimal calibration factor for mutual information effective SINR mapping scheme in 5G NR
Authors: Kim Hyeongseok , Seol Kwon , Kim Jeongchang 
Published: April 2024

AbstractIn this letter, an optimal calibration factor for mutual information effective signal‐to‐interference‐plus‐noise ratio (SINR) mapping (MIESM) scheme in fifth‐generation new radio (5G NR) is provided. By applying the optimal calibration factor to the MIESM scheme, the estimation accuracy of the effective SINR can be improved. The optimal calibration factor minimizes the mean squared error (MSE) of the difference between the estimated effective SINR and the required SNR under additive white Gaussian noise (AWGN) at a block error rate (BLER) of 10%. Simulation results show that the optimized MIESM scheme outperforms the non‐optimized MIESM scheme.
Milne‐Hamming discretized zeroing neural networks for solving time‐varying linear systems
Authors: Chen Xi , Kong Ying , Zeng Xuxiang , Yin Jiayue 
Published: April 2024

AbstractTime‐varying linear systems (TVLS) is a vital mathematical problem in many engineering fields. In this letter, a Milne‐Hamming discretized zeroing neural networks (MHDZNN) is presented to solve the TVLS, where high accurate convergence is got by combing Milne‐Hamming (MH) method. Theoretical analyses of its convergence and precision are discussed to illustrate the validity of the Milne‐Hamming DZNN (MHDZNN). Furthermore, numerical experiments compared with the two existing DZNN models are simulated to verify the superiority of the proposed MHDZNN model.