bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of a novel Bcr/Abl destruction pathway by WP1130 induces apoptosis of chronic myelogenous leukemia cells
Authors: Bartholomeusz Geoffrey A. , Talpaz Moshe , Kapuria Vaibhav , Kong Ling Yuan , Wang Shimei , Estrov Zeev , Priebe Waldemar , Wu Ji , Donato Nicholas J. 
Published: January 2007

Abstract
Imatinib mesylate (Gleevec) is effective therapy against Philadelphia chromosome–positive leukemia, but resistance develops in all phases of the disease. Bcr/Abl point mutations and other alterations reduce the kinase inhibitory activity of imatinib mesylate; thus, agents that target Bcr/Abl through unique mechanisms may be needed. Here we describe the activity of WP1130, a small molecule that specifically and rapidly down-regulates both wild-type and mutant Bcr/Abl protein without affecting bcr/abl gene expression in chronic myelogenous leukemia (CML) cells. Loss of Bcr/Abl protein correlated with the onset of apoptosis and reduced phosphorylation of Bcr/Abl substrates. WP1130 did not affect Hsp90/Hsp70 ratios within the cells and did not require the participation of the proteasomal pathway for loss of Bcr/Abl protein. WP1130 was more effective in reducing leukemic versus normal hematopoietic colony formation and strongly inhibited colony formation of cells derived from patients with T315I mutant Bcr/Abl–expressing CML in blast crisis. WP1130 suppressed the growth of K562 heterotransplanted tumors as well as both wild-type Bcr/Abl and T315I mutant Bcr/Abl–expressing BaF/3 cells transplanted into nude mice. Collectively, our results demonstrate that WP1130 reduces wild-type and T315I mutant Bcr/Abl protein levels in CML cells through a unique mechanism and may be useful in treating CML.
HLA mismatching within or outside of cross-reactive groups (CREGs) is associated with similar outcomes after unrelated hematopoietic stem cell transplantation
Authors: Wade Judith A. , Katovich Hurley Carolyn , Takemoto Steven K. , Thompson John , Davies Stella M. , Fuller Thomas C. , Rodey Glenn , Confer Dennis L. , Noreen Harriet , Haagenson Michael , Kan Fangyu , Klein John , Eapen Mary , Spellman Stephen , Kollman Craig 
Published: January 2007

AbstractThe National Marrow Donor Program maintains a registry of volunteer donors for patients in need of a hematopoietic stem cell transplantation. Strategies for selecting a partially HLA-mismatched donor vary when a full match cannot be identified. Some transplantation centers limit the selection of mismatched donors to those sharing mismatched antigens within HLA-A and HLA-B cross-reactive groups (CREGs). To assess whether an HLA mismatch within a CREG group (“minor”) may result in better outcome than a mismatch outside CREG groups (“major”), we analyzed validated outcomes data from 2709 bone marrow and peripheral blood stem cell transplantations. Three-hundred and ninety-six pairs (15%) were HLA-DRB1 allele matched but had an antigen-level mismatch at HLA-A or HLA-B. Univariate and multivariate analyses of engraftment, graft-versus-host disease, and survival showed that outcome is not significantly different between minor and major mismatches (P = .47, from the log-rank test for Kaplan-Meier survival). However, HLA-A, HLA-B, and HLA-DRB1 allele–matched cases had significantly better outcome than mismatched cases (P < .001). For patients without an HLA match, the selection of a CREG-compatible donor as tested does not improve outcome.
Intravital imaging of fluorescent markers and FRET probes by DNA tattooing
Authors: Bins Adriaan D , van Rheenen Jacco , Jalink Kees , Halstead Jonathan R , Divecha Nullin , Spencer David M , Haanen John BAG , Schumacher Ton NM 
Published: January 2007

Abstract

Background
Advances in fluorescence microscopy and mouse transgenesis have made it possible to image molecular events in living animals. However, the generation of transgenic mice is a lengthy process and intravital imaging requires specialized knowledge and equipment. Here, we report a rapid and undemanding intravital imaging method using generally available equipment.

Results
By DNA tattooing we transfect keratinocytes of living mice with DNA encoding fluorescent biosensors. Subsequently, the behavior of individual cells expressing these biosensors can be visualized within hours and using conventional microscopy equipment. Using this "instant transgenic" model in combination with a corrected coordinate system, we followed the in vivo behavior of individual cells expressing either FRET- or location-based biosensors for several days. The utility of this approach was demonstrated by assessment of in vivo caspase-3 activation upon induction of apoptosis.

Conclusion
This "instant skin transgenic" model can be used to follow the in vivo behavior of individual cells expressing either FRET- or location-based probes for several days after tattooing and provides a rapid and inexpensive method for intravital imaging in murine skin.
A method for detecting the effect of magnetic field on activity changes of neuronal populations of Morimus funereus (coleoptera, cerambycidae)
Authors: Todorović Dajana , Kalauzi Aleksandar , Prolić Zlatko , Jović Miloš , Mutavdžić Dragosav 
Published: January 2007

AbstractModification of a new method for detecting changes in the activities of neuronal population and the nearest neuron is described. Preliminary measurements of the influence of a static magnetic field (2 mT) on neuronal population activity on eight individuals of an endangered insect species Morimus funereus are included. Five minutes exposure produced both excitatory (5/8) and inhibitory (3/8) effect on the activity of neuronal population of M. funereus antennal lobe. However, when the reversibility of induced effects was quantitatively analyzed, our results showed that they were prevailingly irreversible: (7/8) for the population, (6/8) for the nearest neuron. Bioelectromagnetics 28:238–241, 2007. © 2007 Wiley‐Liss, Inc.
Outcome of patients with huge hepatocellular carcinoma after primary resection and treatment of recurrent lesions
Authors: Lee S G , Hwang S , Jung J P , Lee Y J , Kim K H , Ahn C S 
Published: January 2007

Abstract

Background
Tumour recurrence is common after hepatic resection of hepatocellular carcinomas (HCCs) greater than 10 cm in diameter. This study evaluated the outcome of patients with huge HCC after primary resection and treatment of recurrent lesions.

Methods
A retrospective review was undertaken of clinical data for 100 patients with huge HCC who underwent liver resection.

Results
Mean(s.d.) tumour diameter was 13·3(3·0) cm; 80 per cent were single lesions. Systematic and non-systematic resections were performed in 80 and 20 per cent of patients respectively, with R0 resection achieved in 86 per cent. Overall 1-, 3- and 5-year disease-free survival rates were 43, 26 and 20 per cent respectively. Risk factors for HCC recurrence were resection margin less than 1 cm and macrovascular invasion. Extensive tumour necrosis of 90 per cent or more after preoperative transarterial chemoembolization was not a prognostic factor. Some 85 per cent of patients with recurrence received various treatments, and these patients had a longer post-recurrence survival than those who were not treated. Overall 1-, 3- and 5-year survival rates were 66, 44 and 31 per cent respectively.

Conclusion
In patients with huge HCC, hepatic resection combined with active treatment for recurrence resulted in longer-term survival. Frequent protocol-based follow-up appears to be beneficial for the early detection and timely treatment of recurrence.
Detection of tumor‐specific DNA in blood and bone marrow plasma from patients with prostate cancer
Authors: Schwarzenbach Heidi , Chun Felix K.‐H. , Lange Imke , Carpenter Sebastian , Gottberg Miriam , Erbersdobler Andreas , Friedrich Martin G. , Huland Hartwig , Pantel Klaus 
Published: January 2007

AbstractTumor tissues, blood plasma and bone marrow (BM) aspirates of 57 prostate cancer patients (PCa) without clinical signs of overt metastases were assessed for LOH (loss of heterozygosity) by a PCR‐based fluorescence microsatellite analysis, using a panel of 15 markers. Additionally, micrometastatic tumor cells in BM were monitored by an immunocytological cytokeratin assay. In total, 25 (44%), 32 (56%) and 41 (72%) of the patients had at least 1 LOH in their blood, BM and tumor samples, respectively. Among the informative cases, the frequency of LOH was highest in blood plasma for the markers D8S360 (18%) and D10S1765 (15%), and in BM plasma for THRB (24%) and D8S137 (22%). Comparison of blood plasma and BM with tumors showed discrepant results in 35% and 45% of patients, respectively. Whereas all LOHs at THRB in BM plasma were also detected in the autologous tumor tissues, LOHs at D6S474 and D11S898 in BM were not retrieved in the tumors. The comparison with established risk factors showed a correlation of borderline significance for LOH at D9S1748 in the BM aspirates (p = 0.055) and a significant correlation in the tumor samples (p = 0.004) with increasing pathologic Gleason scores. Interestingly, 22% of the PCa patients harbored tumor cells in their BM and tended (p = 0.065) to have more frequent LOH (16%) in BM plasma compared to patients without tumor cells (9%). These data demonstrate, for the first time, the presence of free tumor‐specific DNA in blood and BM of PCa patients and suggest a possible relationship to BM micrometastasis. © 2006 Wiley‐Liss, Inc.
Isolation and quantitation of lectins from vegetable oils
Authors: Klurfeld David M. , Kritchevsky David 
Published: January 2007

AbstractThe factor(s) responsible for the unexplained atherogenicity of peanut oil remain to be elucidated. To this end, we developed a technique to determine if lectin was present in the oil and to quantitate its concentration. This technique was applied to other vegetable oils including corn, soybean, and sunflower. Crude, unprocessed corn and soybean oils were also analyzed for lectin content. The crude oils contained from 858 to 2983 μg lectin per kg, while the refined oils contained 24 to 55 μg/kg of biologically active lectin. The identities of the isolated lectins were confirmed by electrophoresis on SDS‐polyacrylamide gels. The biological significance of the presence of lectin in these oils remains to be determined.
Effects of cholesterol and 25‐hydroxycholesterol on smooth muscle cell and endothelial cell growth
Authors: Cox Donald C. , Comi Karen , Goldstein Ann L. 
Published: January 2007

AbstractAuto‐oxidation products of cholesterol may play a role in atherogenesis. In order to determine whether cholesterol or 25‐hydroxycholesterol, a cholesterol auto‐oxidation product, affected growth of vessel wall cells, sparse and confluent cultures of rabbit thoracic aorta smooth muscle cells and human umbilical vein endothelial cells were exposed to these compounds for 88 hr. The compounds were administered at 10−4, 10−5, 10−6 or 10−7 M in either ethanol or fetal bovine serum (FBS) vehicle. Cells were counted electronically, and the results were expressed as the percent growth in experimental vs control wells. Cholesterol did not inhibit cell growth under any experimental condition. 25‐Hydroxycholesterol had the following effects: inhibited confluent smooth muscle cell growth at 10−4 M in ethanol vehicle only; inhibited sparse smooth muscle cell growth in a dose‐related manner at 10−4, 10−5 and 10−6 M in ethanol vehicle, but in FBS vehicle inhibited at only 10−4 and 10−5 M; inhibited confluent human umbilical vein endothelial cells at 10−4 M in ethanol vehicle only; and inhibited sparse human umbilical vein endothelial cell growth at 10−4 and 10−5 M in ethanol vehicle only. Thus, rabbit aortic smooth muscle cell growth was more sensitive to inhibition by 25‐hydroxycholesterol than human umbilical vein endothelial cell growth was.
Fatty acid composition of seeds from the AustralianAcacia species
Authors: Brown A. J. , Cherikoff V. , Roberts D. C. K. 
Published: January 2007

AbstractPresented are the lipid content and fatty acid composition of 20 species of edible AustralianAcacia seeds. Aborigines reportedly have used at least 18 of these as foods. Seed lipid content ranged from 3% to 22%, with an average of 11% on a dry weight basis. Linoleic (12–71%), oleic (12–56%) and plamitic (7–35%) acids were the major fatty acids. Smaller proportions of behenic, stearic and vaccenic acids were detected. Seventeen of the 20 species were found to have polyunsaturated to saturated (P/S) fatty acid ratios greater than 1, with four species having ratios in excess of 4. The persistent arils attached to the seeds of certain AustralianAcacias and containing a portion of the total lipid were associated with a significantly reduced proportion of linoleic acid in the total seed material. This observation was explained by the aril lipid possessing a markedly different fatty acid composition from that of the seed lipid. For comparison, seeds from two non‐AustralianAcacia species (A. farnesiana andA. cavenia) were analyzed. Australian and non‐Australian were found to exhibit markedly different fatty acid profiles.
Effect of cholestyramine on bile acid metabolism in conventional rats
Authors: Imai Yasuharu , Kawata Sumio , Inada Masami , Miyoshi Shio , Minami Yuzo , Matsuzawa Yuji , Uchida Kiyohisa , Tarui Seiichiro 
Published: January 2007

AbstractEffects of cholestyramine on biliary secretion of cholesterol, phospholipids and bile acids and fecal excretion of sterols and bile acids were examined in Wistar male rats. Six rats were fed a basal diet, and the other six were fed a basal diet supplemented with 5% cholestyramine for eight days. Bile flow and biliary secretion of bile acids and phospholipids (per hour per rat) decreased with cholestyramine treatment, while biliary cholesterol secretion (per hour per rat) remained unchanged. In the biliary bile acid composition, a marked increase of chenodeoxycholic acid with a concomitant decrease of β‐muricholic acid was observed in cholestyramine‐treated rats. Fecal excretion of total sterols and bile acids increased about three‐and four‐fold, respectively, after cholestyramine treatment. The increase of fecal bile acids derived from cholic acid was more predominant than that derived from chenodeoxylcholic acid, resulting in an increase of the cholic acid group/chenodeoxycholic acid group ratio.
Lipid peroxidation in rat tissue slices: Effect of dietary vitamin E, corn oil‐lard and menhaden oil
Authors: Leibovitz Brian E. , Hu Miao‐Lin , Tappel Al L. 
Published: January 2007

AbstractRats were fed for 5 weeks either 10% (w/w) menhaden oil (MO) or a 10% corn oil‐lard (COL) mixture (1∶1) in diets with ≤5 IU or ≤2 IU/kg vitamin E, respectively, or the same diets supplemented with d‐α‐tocopheryl succinate to a total of 35 and 180 IU vitamin E/kg, respectively. Slices of liver and heart from these rats were used to study lipid peroxidationin vitro. Thiobarbituric acid‐reactive substances (TBARS) were measured in the medium after incubation of the slices at 37°C for 1 hr in the absence (uninduced) and presence of 0.5 mM tert‐butyl hydroperoxide (induced). The release of TBARS from slices of heart and liver from rats fed either lipid decreased with increasing levels of dietary vitamin E. At the same level of dietary vitamin E, TBARS release was greater for slices of liver and heart from the MO‐fed rats than from the COL‐fed rats. Application of the TBARS data to a model simulating the experimental conditions showed a good correlation (r=0.95, p<0.001) between experimental and simulated values. Of the 16∶0–22∶6 fatty acids measured in liver from MO‐fed rats, 15.4% was n−6 fatty acids and 29.9% was n−3 fatty acids; in liver from COL‐fed rats, the respective values were 37.4% and 3.7%. Liver and kidney vitamin E levels were unaffected by the dietary lipid. This study demonstrated that a dietary fish oil increased the susceptibility of rat liver and heart toin vitro lipid peroxidation, and that vitamin E decreased TBARS in tissues from rats fed COL to lower levels than for tissues from rats fed MO. The results suggest that there might also be an increased requirement for dietary antioxidants by humans using fish oil supplements.
A tetrameric dialdehyde formed in the reaction of butyral dehyde and benzylamine: A possible intermediary component for protein cross‐linking induced by lipid oxidation
Authors: Kikugawa Kiyomi , Kato Tetsuta , Iwata Atsushi 
Published: January 2007

AbstractWe investigated the reaction of butyraldehyde and benzylamine and analyzed the products to identify the components that produce protein cross‐linking in the reaction of butyraldehyde and proteins. When the mixtures of butyraldehyde and benzylamine were incubated at pH 7 and 37°C for 48 hr, many reaction products other than 2‐ethyl‐2‐hexenal and Schiff bases of butyraldehyde and 2‐ethyl‐2‐hexenal were produced. Fluorescent substance(s) were formed only in the presence of dissolved oxygen in the reaction mixture. Three new nonflourescent products—d,e andf—were isolated, and their structures are suggested to be 2,9‐dibenzyl‐4,6,8‐triethyl‐7‐propyl‐2,9‐diazabicyclo[3,3,1] nona‐3‐ene (d), 1‐phenyl‐2‐benzyl‐ 4,5,7‐triethyl‐6‐propyl‐1H,2H,3H,5H,6H,7H,8H‐pyrido[1,2‐clpyrimidine (e) and 1‐phenyl‐2‐benzyl‐4,5,7‐triethyl‐6‐propyl‐1H,2H,4aH,5H,6H,7H,8H‐pyrido[1,2‐c]pyrimidine (f). Formation of these compounds suggested that the protein, cross‐linking with butyraldehyde is caused by the tretrameric dialdehyde formed by repeated aldol condensation and Michael reaction of butyraldehyde.
Prostaglandin H synthase-dependent mutagenic activation of heterocyclic aromatic amines of the IQ-type
Authors: Wild D. , Degen G.H. 
Published: January 2007

Abstract
Microsomes from ram seminal vesides known as a rich source of prostaglandin H synthase (PHS) activate the food mutagen IQ (2-amino-3-methylimidazo[4,5-f]quinoline) to (a) product(s) mutagenic in Salmonella typhimurium TA98. The activation is dependent on the PHS cofactor arachidonic acid and is strongly inhibited by the PHS inhibitor indomethacin. In this system, the mutagenic potency of IQ is 22 and 110 times higher than that of 2-aminofluorene and benzidine, respectively. The high mutagenic potency of IQ observed previously with mono-oxygenase activation is thus extended to the PHS system. The mutagenic activity produced by PHS increases for 4 h; this contrasts with the relatively short lifetime of the activity produced by mono-oxygenase and suggests that different agents are involved in the two processes. The PHS-mediated mutagenic activity of IQ is strongly dependent on the bacterial O -acetyltransferase which is defective in strain TA98/1,8-DNP 6 . Further, the responses of the strains TA1978 and TA1538 indicate that the mutagenic activity is dependent on lack of the bacterial DNA excision repair and independent of the plasmid pkM101 coded error-prone DNA repair system. Structural analogs of IQ without a methyl group on the imidazole ring and with a naphthalene instead of the quinoline ring show greatly diminished PHS-mediated mutagenic activity. The strain response pattern and structure-activity relationships are similar to those found with mono-oxygenase activation of IQ and thus indicate a basic similarity of the IQ activation via PHS with that via mono-oxygenase. It is hypothesized that PHS may activate carcinogenic heterocyclic aromatic amines in vivo .
Successful Food‐Based Programmes, Supplementation and Fortification
Authors: Mannar MG Venkatesh 
Published: January 2007

ABSTRACTThis review highlights interventions and delivery mechanisms to alleviate macro‐ and micronutrient deficiencies in preschool children. These deficiencies can be addressed through an integrated combination of improved dietary intake, supplementation, commercial and home‐based fortification of complementary foods. Several developed and developing countries have implemented successful approaches to eliminate protein‐energy malnutrition and micronutrient deficiencies with sustained impact. These include provision of oral supplements in capsule, tablet or syrup form. Certain micronutrients (eg, vitamin A) can be provided as high‐dose supplements twice per year. Most other vitamins and minerals (eg, iron, zinc, iodine) need to be provided in daily doses. Fortified complementary foods provided through public feeding programmes and commercially marketed foods have also made a positive impact. There is growing evidence of the impact of home‐based fortification of complementary foods using premixes in single‐serving sachets. The fortification of commercially marketed staple foods such as cereal flours, cooking oils and dairy products could have a small but significant impact on preschool children. Cereal flours with iron, folic acid and other nutrients have expanded rapidly, with evidence of impact. A key need is to deliver micronutrients to remote and impoverished populations in an affordable and sustainable manner. Government commitment, clear policy and programme direction, advocacy and communication combined with a strong public–private partnership is essential for successful programmes. Often a period of voluntary fortification needs to be followed by mandatory requirement to ensure full compliance and sustained impact. The review concludes that proven technologies, communications and infrastructure can be harnessed to ensure that the nutrient needs of preschool children are met. When administered systematically with the commitment of and participation by the public and private sectors, most of the major deficiencies can be bridged on a sustained basis, contributing to improved health and well‐being of millions of children around the world.