bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epistemología, enfermería en la praxis investigativa
Authors: Santana Silvia , Desteffano Silvia 
Published: February 2024

Al profundizar en la naturaleza científica de la enfermería, es posible analizar que la epistemología se convierte en una herramienta para ayudar al trabajo del profesional de la salud, quien encuentra mejores opciones para tomar decisiones, enfrentar situaciones, ayudar a los pacientes e impactar positivamente en el entorno donde trabaja. La enfermería en su carácter emergente muestra que la práctica investigativa confiere a los profesionales de la salud el fortalecimiento de las ciencias médicas, pues además de abrir espacios para el aprendizaje, minimiza riesgos e incluso evita obstáculos para apoyar la salud de los enfermos. En tal sentido, en esta investigación se presentan los siguientes objetivos: Descubrir los aportes de la epistemología en la práctica investigativa de la enfermería y Revelar las perspectivas actuales de la enfermería como práctica investigativa, tomando en cuenta la base teórica de la teoría socio-cultural de Lev Vygotsky y la construcción del conocimiento de Robert Gané, se realizó un proceso de revisión documental que condujo a sendas conclusiones que indican que el componente del conocimiento personal desde la concepción epistemológica de la praxis investigativa en la enfermería muestra un uso terapéutico personal o de relación transpersonal. Este componente es capaz de llevar a interacciones, transacciones e interacciones entre ambos elementos: clientes y enfermeros, cuyos instrumentos permiten direccionar acciones hacia el cuidado y resguardo de la salud de todas las personas que interactúan en los centros hospitalarios.
Mathematical modelling of antibiotic interaction on evolution of antibiotic resistance: an analytical approach
Authors: Nashebi Ramin , Sari Murat , Kotil Seyfullah Enes 
Published: February 2024

Background
The emergence and spread of antibiotic-resistant pathogens have led to the exploration of antibiotic combinations to enhance clinical effectiveness and counter resistance development. Synergistic and antagonistic interactions between antibiotics can intensify or diminish the combined therapy’s impact. Moreover, these interactions can evolve as bacteria transition from wildtype to mutant (resistant) strains. Experimental studies have shown that the antagonistically interacting antibiotics against wildtype bacteria slow down the evolution of resistance. Interestingly, other studies have shown that antibiotics that interact antagonistically against mutants accelerate resistance. However, it is unclear if the beneficial effect of antagonism in the wildtype bacteria is more critical than the detrimental effect of antagonism in the mutants. This study aims to illuminate the importance of antibiotic interactions against wildtype bacteria and mutants on the deacceleration of antimicrobial resistance.

Methods
To address this, we developed and analyzed a mathematical model that explores the population dynamics of wildtype and mutant bacteria under the influence of interacting antibiotics. The model investigates the relationship between synergistic and antagonistic antibiotic interactions with respect to the growth rate of mutant bacteria acquiring resistance. Stability analysis was conducted for equilibrium points representing bacteria-free conditions, all-mutant scenarios, and coexistence of both types. Numerical simulations corroborated the analytical findings, illustrating the temporal dynamics of wildtype and mutant bacteria under different combination therapies.

Results
Our analysis provides analytical clarification and numerical validation that antibiotic interactions against wildtype bacteria exert a more significant effect on reducing the rate of resistance development than interactions against mutants. Specifically, our findings highlight the crucial role of antagonistic antibiotic interactions against wildtype bacteria in slowing the growth rate of resistant mutants. In contrast, antagonistic interactions against mutants only marginally affect resistance evolution and may even accelerate it.

Conclusion
Our results emphasize the importance of considering the nature of antibiotic interactions against wildtype bacteria rather than mutants when aiming to slow down the acquisition of antibiotic resistance.
Genome-wide association studies reveal stable loci for wheat grain size under different sowing dates
Authors: Hong Yi , Zhang Mengna , Yuan Zechen , Zhu Juan , Lv Chao , Guo Baojian , Wang Feifei , Xu Rugen 
Published: February 2024

Background
Wheat (Tritium aestivum L.) production is critical for global food security. In recent years, due to climate change and the prolonged growing period of rice varieties, the delayed sowing of wheat has resulted in a loss of grain yield in the area of the middle and lower reaches of the Yangtze River. It is of great significance to screen for natural germplasm resources of wheat that are resistant to late sowing and to explore genetic loci that stably control grain size and yield.

Methods
A collection of 327 wheat accessions from diverse sources were subjected to genome-wide association studies using genotyping-by-sequencing. Field trials were conducted under normal, delayed, and seriously delayed sowing conditions for grain length, width, and thousand-grain weight at two sites. Additionally, the additive main effects and multiplicative interaction (AMMI) model was applied to evaluate the stability of thousand-grain weight of 327 accessions across multiple sowing dates.

Results
Four wheat germplasm resources have been screened, demonstrating higher stability of thousand-grain weight. A total of 43, 35, and 39 significant MTAs were determined across all chromosomes except for 4D under the three sowing dates, respectively. A total of 10.31% of MTAs that stably affect wheat grain size could be repeatedly identified in at least two sowing dates, with PVE ranging from 0.03% to 38.06%. Among these, six were for GL, three for GW, and one for TGW. There were three novel and stable loci (4A_598189950, 4B_307707920, 2D_622241054) located in conserved regions of the genome, which provide excellent genetic resources for pyramid breeding strategies of superior loci. Our findings offer a theoretical basis for cultivar improvement and marker-assisted selection in wheat breeding practices.
An alternative peptone preparation using Hermetia illucens (Black soldier fly) hydrolysis: process optimization and performance evaluation
Authors: Liu Gaoqiang , Tiang Ming Foong , Ma Shixia , Wei Zeyan , Liang Xiaolin , Sajab Mohd Shaiful , Abdul Peer Mohamed , Zhou Xueyan , Ma Zhongren , Ding Gongtao 
Published: February 2024

Background
Hermetia illucens (HI), commonly known as the black soldier fly, has been recognized for its prowess in resource utilization and environmental protection because of its ability to transform organic waste into animal feed for livestock, poultry, and aquaculture. However, the potential of the black soldier fly’s high protein content for more than cheap feedstock is still largely unexplored.

Methods
This study innovatively explores the potential of H. illucens larvae (HIL) protein as a peptone substitute for microbial culture media. Four commercial proteases (alkaline protease, trypsin, trypsase, and papain) were explored to hydrolyze the defatted HIL, and the experimental conditions were optimized via response surface methodology experimental design. The hydrolysate of the defatted HIL was subsequently vacuum freeze-dried and deployed as a growth medium for three bacterial strains (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) to determine the growth kinetics between the HIL peptone and commercial peptone.

Results
The optimal conditions were 1.70% w/w complex enzyme (alkaline protease: trypsin at 1:1 ratio) at pH 7.0 and 54 °C for a duration of 4 h. Under these conditions, the hydrolysis of defatted HIL yielded 19.25% ±0.49%. A growth kinetic analysis showed no significant difference in growth parameters (μmax, Xmax, and λ) between the HIL peptone and commercial peptone, demonstrating that the HIL hydrolysate could serve as an effective, low-cost alternative to commercial peptone. This study introduces an innovative approach to HIL protein resource utilization, broadening its application beyond its current use in animal feed.
New records of immature aquatic Diptera from the Foulden Maar Fossil-Lagerstätte, New Zealand, and their biogeographic implications
Authors: Baranov Viktor O. , Haug Joachim T. , Kaulfuss Uwe 
Published: February 2024

Background
The biogeographical and ecological history of true flies (Diptera) in New Zealand is little known due to a scarcity of fossil specimens. Here, we report a fauna of immature aquatic dipterans from freshwater diatomites of the early Miocene Foulden Maar Fossil-Lagerstätte in southern New Zealand.

Methods
We document 30 specimens of immature dipterans, mostly pupae, and compare their external morphology to extant aquatic Diptera. Based on the reconstructed paleoenvironment of Foulden Maar, we discuss taxonomic, ecological and taphonomic implications of this early Miocene fauna.

Results
Among Chironomidae, one pupal morphotype is attributed to Tanypodinae, one pupal morphotype and one larval morphotype are placed into Chironomus (Chironominae) and a further morphotype into Chironominae incertae sedis. Chaoboridae are represented by a pupal morphotype congeneric or very close to the extant Chaoborus, today globally distributed except for New Zealand. Additional immature specimens are likely larvae and puparia of brachyceran flies but cannot be identified to a narrower range. These finds document an aquatic dipteran fauna in New Zealand in the earliest Miocene and highlight Neogene extinction as a factor in shaping the extant Diptera fauna in New Zealand. Immature aquatic dipterans were a common and likely ecologically important component of the early Miocene Foulden Maar lake. Preservation of larvae and pupae may have been promoted by diatomaceous microbial mats and the light colour of the diatomite likely facilitated spotting of these minute fossils in the field.
The Effect of Liquidity and Capital Intensity on Tax Aggressiveness
Authors: Astrina Fenty , Sunardi  , Pertiwi Anggun Putri 
Published: February 2024

Aims: This research aims to determine and analyze the effect of liquidity and capital intensity on tax aggressiveness.
Study Design: The population in this study are Basic Industry and Chemical sub-sector manufacturing companies listed on the Indonesia Stock Exchange in the 2020-2022 period, namely 66 companies. The sampling method was purposive sampling. The final sample in this research was 22 companies and the total final sample was 66 samples. The data used is secondary data taken from the financial statements obtained from the Indonesian Stock Exchange.
Methodology: The data collection method is document analysis. The data analysis method used is quantitative analysis. The analysis techniques in this research are the classical assumption test, determinant coefficient test and Multiple Linear Regression.
Results: The results of this research show that liquidity influences tax aggressiveness, capital intensity influences tax aggressiveness.
Conclusion: The research results show that liquidity, capital intensity simultaneously affect tax aggressiveness. liquidity has a positive and significant effect on tax aggressiveness. Meaning that Companies with high liquidity are healthy companies with large tax liabilities so they have a higher potential for aggressive tax action. so does Capital Intensity has a positive and significant effect on Tax Aggressiveness. Meaning that higher fixed asset investment is carried out as a form of reducing profits and increasing depreciation expenses, thereby reducing the tax burden
Bio-prospecting the Efficacy of Carrier Based Inoculant of Rhizobium Strains as Plant Growth Promoter for the Development of Albizia procera Seedling
Authors: Ghabru Arti , Rana Neerja , Meenakshi  , Verma Geeta 
Published: February 2024

The carrier-based formulation of rhizobial cultures in general, excels to register their effects on various plant growth parameters of Albizia procera seedlings. Carrier based bio-inoculant prepared with rhizobial isolate FA6 of Uttrakhand performed better for all plant growth characteristics. Charcoal based application of rhizobial isolates also increased the N, P, K content of soil. In case of seedlings treated with charcoal-based formulation of rhizobial isolate from Himachal Pradesh maximum available N (323.30 kg/ha), P (38.97 kg/ha) and K (395.9 kg/ha) content was recorded in T2 (Charcoal based formulation of BA2 strain+20kg/ha N) which was significantly superior to T3 and uninoculated control. Among the charcoal-based formulation of rhizobial isolate from Uttrakhand seedlings under treatment T2 (Charcoal based formulation of FA6 strain+20kg/ha N) registered maximum available N (327.7kg/ha), P (39.01 kg/ha) and K (397.4 kg/ha) content in soil. Charcoal based application of rhizobial isolates significantly influenced the activity of soil enzymes (Dehydrogenase and Phosphatase). Application of Carrier based formulation of rhizobial isolate BA2 from Himachal Pradesh showed maximum dehydrogenase (11.20 μg TPF g-1h-1) and phosphatase (536.2 μmolL-1g-1h-1) activity as compare to uninoculated control. Whereas, among the rhizobial isolates from Uttrakhand maximum dehydrogenase (11.39 μg TPFg-1h-1) and phosphatase (545.4 μmol L-1g-1h-1) activity was also recorded with T2 (charcoal-based formulation of strain FA6+20kg/ha N) which was significantly superior to T3 and uninoculated control.
Isolation of Mast Cell Granules
Authors: Lindstedt Ken A. , Kovanen Petri T. 
Published: January 2006

AbstractThe mast cell is a multipotent inflammatory cell that has been shown to participate in the pathogenesis of a variety of diseases, such as immediate hypersensitivity reactions, arthritis, atherosclerosis, and heart failure. Upon stimulation, mast cells exocytose cytoplasmic secretory granules into their extracellular microenvironment. These granules are modified lysosomes containing preformed mediators such as histamine, neutral proteases, cytokines, and growth factors embedded in a heparin proteoglycan matrix. When exposed to the extracellular fluid, the soluble components of the granules (e.g., histamine and cytokines) diffuse away, whereas the heparin proteoglycans and the mast cell–specific neutral proteases (e.g., chymase) remain tightly bound to each other, forming proteolytically active intra‐ and extracellular granule remnants. This unit describes a method to isolate rat serosa mast cell granule remnants in experiments aimed at determining the role of mast cell activation and degranulation in disease.
Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation (BiFC) Analysis
Authors: Hu Chang‐Deng , Grinberg Asya V. , Kerppola Tom K. 
Published: January 2006

AbstractProtein interactions integrate stimuli from different signaling pathways and developmental programs. Bimolecular fluorescence complementation (BiFC) analysis has been developed for visualization of protein interactions in living cells. This approach is based on complementation between two fragments of a fluorescent protein when they are brought together by an interaction between proteins fused to the fragments, and it enables visualization of the subcellular locations of protein interactions in the normal cellular environment. It can be used for the analysis of many protein interactions and does not require information about the structures of the interaction partners. A multicolor BiFC approach has been developed for simultaneous visualization of interactions with multiple alternative partners in the same cell, based on complementation between fragments of engineered fluorescent proteins that produce bimolecular fluorescent complexes with distinct spectral characteristics. This enables comparison of subcellular distributions of different protein complexes in the same cell and allows analysis of competition between mutually exclusive interaction partners.
The Human Gene Mutation Database (HGMD) and Its Exploitation in the Study of Mutational Mechanisms
Authors: Cooper David N. , Stenson Peter D. , Chuzhanova Nadia A. 
Published: January 2006

AbstractThe Human Gene Mutation Database (HGMD) constitutes a comprehensive core collection of data on germ‐line mutations in nuclear genes underlying or associated with human inherited disease (http://www.hgmd.org). Data cataloged include single base‐pair substitutions in coding, regulatory, and splicing‐relevant regions, microdeletions and microinsertions, indels, and triplet repeat expansions, as well as gross gene deletions, insertions, duplications, and complex rearrangements. Each mutation is entered into HGMD only once, in order to avoid confusion between recurrent and identical‐by‐descent lesions. By June 2005, the database contained in excess of 53,000 different lesions detected in 2029 different nuclear genes, with new entries currently accumulating at a rate in excess of 5000 per annum. HGMD includes cDNA reference sequences, now provided for more than 90% of the listed genes, splice junction data, disease‐associated and functional polymorphisms, and links to data present in publicly available online locus‐specific mutation databases.
Materialien für Sensorpolymere: Möglichkeiten kombinatorischen Designs
Authors: Potyrailo Radislav A. 
Published: January 2006

AbstractGegenwärtig wird intensiv nach Möglichkeit gesucht, die Selektivität, Ansprechzeit und Empfindlichkeit bei chemischen oder biologischen Nachweisen in Gasen oder Flüssigkeiten zu steigern. Ein besonderes Augenmerk liegt hierbei auf Materialien für Sensorpolymere, die vielfältig einsetzbar sind und sich für Sensoren mit ganz unterschiedlicher Energiewandlung eignen, z. B. für elektrische, mechanische, Strahlungs‐ und Wärmeenergie. Die Vielfalt resultiert aus den zahlreichen Parametern solcher Materialien, die an spezifische Anforderungen angepasst werden können. Mit zunehmender struktureller und funktionaler Komplexität polymerer Sensormaterialien wird es allerdings immer schwieriger, gewünschte Eigenschaften gezielt einzustellen. Zentrale Forschungsthemen betreffen Homo‐ und Copolymere, Formulierungen für Sensormaterialien, gezielt strukturierte Materialien und molekulare Erkennung, wobei in allen Fällen kombinatorische Methoden und Hochdurchsatzverfahren zum Einsatz kommen. Wir analysieren hier den gegenwärtigen Stand der Technik, berichten über Entwicklungstrends bei Sensorpolymeren und zeigen verbliebene Wissenslücken auf.
Expression of Cux‐1 and Cux‐2 in the developing somatosensory cortex of normal and barrel‐defective mice
Authors: Ferrere Arnaud , Vitalis Tania , Gingras Hélène , Gaspar Patricia , Cases Olivier 
Published: January 2006

AbstractRecently, two orthologues of the Drosophila homeobox Cut gene, Cux‐1 and Cux‐2, have been identified as restricted molecular markers of upper layer (II–IV) neurons in the murine cerebral cortex. We show that during early postnatal life, from P0 to P10, Cux‐1 and Cux‐2 mRNA are coexpressed in all primary sensory cortices. Antisera to Cux‐1 and Cux‐2 immunoreactivities preferentially label neurons in the barrel walls of the primary somatosensory cortex (S1). Subsequently, Cux‐1 remains enriched in sensory cortices, whereas Cux‐2 expression enlarges to comprise the frontal and insular areas. The laminar distribution of Cux‐1 and Cux‐2 differs: Cux‐1 follows a layer IV to layer II decreasing gradient of expression, whereas Cux‐2 expression is homogeneous across layers IV–II. No colocalization was found with GABA and birth dating experiments showed that Cux‐1–positive neurons in layer IV are born during a restricted period, E13.5–E14.5, suggesting that Cux‐1 is a useful molecular marker of the glutamatergic neurons of layer IV. We examined Cux‐1 and Cux‐2 in barrel‐defective mouse strains, the VMAT2 KO, the MAOA KO, and the Adcyl 1brl strain. A normal expression level of Cux‐1 and Cux‐2 was found in layer IV, despite the lack of segregation of the neurons as barrels. Conversely, in Reeler mice, Cux‐1 and Cux‐2 had a distinct laminar distribution: the Cux‐1–positive neurons had an inverted deep localization, whereas the Cux‐2–positive neurons were distributed throughout the cortical thickness, suggesting that Cux‐2 expression is more widely expressed in the inverted cortex of reeler mutants. Our results indicate that Cux‐1 is a useful marker of the layer IV neurons in S1, and that Cux‐1 and Cux‐2 are differently regulated in the upper layers of the cerebral cortex. © 2006 Wiley‐Liss, Inc.
Bioluminescent monitoring of turbulent bioconvection
Authors: Šimkus R. 
Published: January 2006

AbstractUnder adjusted experimental conditions, open‐to‐air cultures of lux gene‐engineered Ralstonia eutropha (wholecell biosensors of copper) exhibit bioconvection, which accounts for fluctuating bioluminescence. The power spectrum of bioluminescence intensity fluctuations recorded from a cylindrical sample 9 mm in diameter and ∼10 mm in height is characterized by a dominant low‐frequency oscillation (with a characteristic period of ∼8–12 min), which is occasionally accompanied by a few weaker oscillations. The corresponding spectral peaks emerge on a high‐noise background. The spectra of bioluminescence intensity fluctuations qualitatively resemble the spectra of temperature or fluid velocity fluctuations in an appropriate turbulent thermal convection system. It has been suggested that in a bioconvective system, like in thermal convection systems, the emergence of oscillation reflects the large‐scale convective circulation that spans the height of the cylindrical cell. The velocity of large‐scale bioconvective circulation was estimated to be 37–48 µm/s. The occasional emergence of weaker‐than‐dominant oscillations was explained through the coexistence and interaction of the large‐scale circulation with, presumably, a gene‐expression‐related cyclic process (with a characteristic period of ∼25–50 min). Copyright © 2006 John Wiley & Sons, Ltd.