bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Variations in
Klebsiella pneumoniae
and
Escherichia coli
FimH Affect Function and Pathogenesis in the Urinary Tract
Authors: Rosen David A. , Pinkner Jerome S. , Walker Jennifer N. , Elam Jennifer Stine , Jones Jennifer M. , Hultgren Scott J. 
Published: May 2008

ABSTRACT

Type 1 pili mediate binding, invasion, and biofilm formation of uropathogenic
Escherichia coli
(UPEC) in the host urothelium during urinary tract infection (UTI) via the adhesin FimH. In this study, we characterized the molecular basis of functional differences between FimH of the UPEC isolate UTI89 and the
Klebsiella pneumoniae
cystitis isolate TOP52. Type 1 pili characteristically mediate mannose-sensitive hemagglutination of guinea pig erythrocytes. Although the adhesin domain of
K. pneumoniae
TOP52 FimH (FimH
52
) is highly homologous to that of
E. coli
, with an identical mannose binding pocket and surrounding hydrophobic ridge, it lacks the ability to agglutinate guinea pig erythrocytes. In addition, FimH-dependent biofilm formation in
K. pneumoniae
is inhibited by heptyl mannose, but not methyl mannose, suggesting the need for contacts outside of the mannose binding pocket. The binding specificity differences observed for FimH
52
resulted in significant functional differences seen in the pathogenesis of
K. pneumoniae
UTI compared to
E. coli
UTI. Infections in a murine model of UTI demonstrated that although the
K. pneumoniae
strain TOP52 required FimH
52
for invasion and IBC formation in the bladder, FimH
52
was not essential for early colonization. This work reveals that a limited amount of sequence variation between the FimH of
E. coli
and
K. pneumoniae
results in significant differences in function and ability to colonize the urinary tract.
The Oligomerization Domain of C4-Binding Protein (C4bp) Acts as an Adjuvant, and the Fusion Protein Comprised of the 19-Kilodalton Merozoite Surface Protein 1 Fused with the Murine C4bp Domain Protects Mice against Malaria
Authors: Ogun Solabomi A. , Dumon-Seignovert Laurence , Marchand Jean-Baptiste , Holder Anthony A. , Hill Fergal 
Published: May 2008

ABSTRACTHighly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses.Plasmodium yoelii19-kDa merozoite surface protein 1 (MSP119) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite challenge. Fusion proteins comprising this antigen fused to the oligomerization domain of the murine complement inhibitor C4-binding protein (C4bp) and a series of homologues have been produced. These C4bp domains acted as adjuvants for the fused antigen; the MSP119-murine C4bp fusion protein induced protective immunity in BALB/c mice. Because this fusion protein also induced antibodies against circulating murine C4bp, distantly related C4bp oligomerization domains fused to the same antigen were tested. These homologous domains did not induce antibodies against murine C4bp and, surprisingly, induced higher antibody titers against the antigen than the murine C4bp domain induced. These results demonstrate a new adjuvantlike effect of C4bp oligomerization domains.
The Chlamydial Plasmid-Encoded Protein pgp3 Is Secreted into the Cytosol ofChlamydia-Infected Cells
Authors: Li Zhongyu , Chen Ding , Zhong Youmin , Wang Shiping , Zhong Guangming 
Published: May 2008

ABSTRACTThe chlamydial cryptic plasmid encodes eight putative open reading frames (ORFs), designated pORF1 to -8. Antibodies raised against these ORF proteins were used to localize the endogenous proteins during chlamydial infection. We found that the pORF5 protein (also known as pgp3) was detected mainly in the cytosol ofChlamydia-infected cells, while the remaining seven proteins were found inside the chlamydial inclusions only. The pgp3 distribution pattern in the host cell cytosol is similar to but not overlapping with that of chlamydial protease/proteasome-like activity factor (CPAF), a chlamydial genome-encoded protein known to be secreted from chlamydial inclusions into the host cell cytosol. The anti-pgp3 labeling was removed by preabsorption with pgp3 but not CPAF fusion proteins and vice versa, demonstrating that pgp3 is a unique secretion protein. This conclusion is further supported by the observation that pgp3 was highly enriched in cytosolic fractions and had a minimal presence in the inclusion-containing nuclear fractions prepared fromChlamydia-infected cells. The pgp3 protein was detected as early as 12 h after infection and was secreted by all chlamydial species that carry the cryptic plasmid, suggesting that there is a selection pressure for maintaining pgp3 secretion during chlamydial infection. Although expression of pgp3 in the host cell cytosol via a transgene did not alter the susceptibility of the transfected cells to the subsequent chlamydial infection, purified pgp3 protein stimulated macrophages to release inflammatory cytokines, suggesting that pgp3 may contribute to chlamydial pathogenesis.
Plasmodium falciparumSTEVOR Proteins Are Highly Expressed in Patient Isolates and Located in the Surface Membranes of Infected Red Blood Cells and the Apical Tips of Merozoites
Authors: Blythe Jane E. , Yam Xue Yan , Kuss Claudia , Bozdech Zbynek , Holder Anthony A. , Marsh Kevin , Langhorne Jean , Preiser Peter R. 
Published: May 2008

ABSTRACTThe human parasitePlasmodium falciparumhas the potential to express a vast repertoire of variant proteins on the surface of the infected red blood cell (iRBC). Variation in the expression pattern of these proteins is linked to antigenic variation and thereby evasion of host antibody-mediated immunity. The genes in thestevormultigene family code for small variant antigens that are expressed in blood-stage parasites where they can be detected in membranous structures called Maurer's clefts (MC). Some studies have indicated that STEVOR protein may also be trafficked to the iRBC membrane. To address the location of STEVOR protein in more detail, we have analyzed expression in several cultured parasite lines and in parasites obtained directly from patients. We detected STEVOR expression in a higher proportion of parasites recently isolated from patients than in cultured parasite lines and show that STEVOR is trafficked in schizont-stage parasites from the MC to the RBC cytosol and the iRBC membrane. Furthermore, STEVOR protein is also detected at the apical end of merozoites. Importantly, we show that culture-adapted parasites do not require STEVOR for survival. These findings provide new insights into the role of thestevormultigene family during both the schizont and merozoite stages of the parasite and highlight the importance of studying freshly isolated parasites, rather than parasite lines maintained in culture, when investigating potential mediators of host-parasite interactions.
Immunization with a Toll-Like Receptor 7 and/or 8 Agonist Vaccine Adjuvant Increases Protective Immunity againstLeishmania majorin BALB/c Mice
Authors: Zhang Wen-Wei , Matlashewski Greg 
Published: May 2008

ABSTRACTActivation of Toll-like receptors (TLRs) on antigen-presenting cells of the innate immune system initiates, amplifies, and directs the antigen-specific acquired immune response. Ligands that stimulate TLRs therefore represent potential vaccine adjuvants. In the present study, we determined whether imiquimod and its related compound R848, which are TLR7 and/or TLR8 agonists, represent potential vaccine adjuvants when delivered topically, subcutaneously, or intramuscularly. Using theLeishmania majorinfection model in BALB/c mice, vaccination with crudeLeishmaniaantigen was not protective against subsequent challenge infection unless it was administered with R848 or a topical application of imiquimod containing cream on the skin. Subcutaneous vaccination with these adjuvants mediated a TH1 response againstL. majorantigen, which appeared to suppress the TH2 response following a challenge infection. Protective immunity was generated following subcutaneous vaccination but not intramuscular vaccination. These observations suggest that topically administered imiquimod or subcutaneously injected R848 represent potential vaccine adjuvants to enhance the TH1 response, which can be used with existing or new vaccine formulations.
Minichromosome maintenance protein 2 (MCM2) is a stronger discriminator of increased proliferation in mucosa adjacent to colorectal cancer than Ki-67
Authors: Hanna-Morris A , Badvie S , Cohen P , McCullough T , Andreyev H J N , Allen-Mersh T G 
Published: May 2008

Background:Loss of control of mucosal crypt cell proliferation resulting in a hyperproliferative field change occurs early in the adenoma-carcinoma sequence. Ki-67, the current gold-standard marker of cellular proliferation, is a cell cycle protein that may lack sensitivity in demonstrating altered mucosal crypt cell dynamics. Minichromosome maintenance protein 2 (MCM2) has a specific role in DNA replication and has been proposed as a new marker for screening for colorectal cancer.Aim:To compare the expression of Ki-67 with that of MCM2 in colorectal mucosa associated with colorectal cancer.Methods:Ki-67 and MCM2 immunostaining was performed on serial sections taken from formalin-fixed, paraffin-embedded specimens. Labelling indices were calculated by counting the proportion of positively stained nuclei in representative areas of adenocarcinoma, and in equivalent superficial, middle and basal crypt compartments of mucosa sampled 1 cm from tumour (Ca1) and 10 cm from tumour (Ca10).Results:Specimens were obtained from 43 patients (27 adenocarcinoma, 16 no-cancer controls). Most nuclei in specimens of adenocarcinoma stained positively for MCM2 and Ki-67. In Ca1 and Ca10 samples, significantly greater staining of MCM2 than Ki-67 was seen in all crypt compartments. Receiver operator characteristic curve analysis suggested that proliferation changes (assessed by either MCM2 or Ki-67 staining) in Ca10, but not in Ca1, mucosa significantly predicted origin from a carcinoma-associated colon.Conclusions:MCM2 was more sensitive than Ki-67 in identifying colorectal mucosal proliferation. Increased proliferation (assessed by either MCM2 or Ki-67 staining) in mucosa at 10 cm, but not at 1 cm, from carcinoma significantly predicted origin from a carcinoma-associated colon.
WHAT APPEARS TO MAKE SEA EFFECTIVE IN DIFFERENT PLANNING SYSTEMS
Authors: GAZZOLA PAOLA 
Published: May 2008

If Strategic Environmental Assessment (SEA) is to develop into an effective decision support instrument globally, then it should not just rely only on the input provided by a selected group of countries. In this context, the understanding of how to apply SEA effectively in different planning systems is unlikely to improve if the knowledge about those systems is limited. This paper establishes what appears to make SEA effective in two countries with different planning systems: Italy and the UK. Italy is chosen as an example, representing a specific Southern-European planning culture, which to date has not contributed to the SEA literature to any great extent. Based on empirical observations, it is suggested that the SEA effectiveness elements portrayed in the international literature are not fully valid. The UK is chosen as an example, which represents a specific Northern-European planning culture. Countries representing this planning culture have contributed heavily to the international SEA literature and have influenced the development of SEA theory strongly. In this context, the SEA effectiveness elements and benefits portrayed in the international literature appear to be valid.As part of a PhD research project, this paper builds on the findings of a content analysis of the international SEA literature (Fischer and Gazzola, 2006).
Hypercoagulability in patients with haematological neoplasia: No apparent initiation by tissue factor
Authors: Iversen Per , Østenstad Bjørn , Iversen Nina , Holme Pål , Sandset Per , Negaard Helene 
Published: May 2008

SummaryPatients with haematological malignancies carry increased risk of venous thrombosis (VT). However, the mechanisms that link these malignancies to activated coagulation have not been fully identified. Since anti-haemostatic agents are studied in clinical trials for their potential to prolong survival in cancer patients, a detailed characterisation of haemostatic markers in cancer subtypes is needed. Hence, in this study, we measured the plasma concentrations and mRNA expression in blood mononuclear cells of haemostatic parameters in 93 patients with haematological neoplasias (acute myeloid leukaemia, chronic lymphatic leukaemia, multiple myeloma, and non-Hodgkin’s lymphoma) before start and after completion of cancer therapy. At diagnosis we found activation of coagulation and fibrinolysis, especially in patients with acute myeloid leukaemia. This hypercoagulation was not associated with increased levels of tissue factor (TF) or factor VII (fVII) antigen or mRNA, or levels of activated fVII. In conclusion we found a hypercoagulable state in patients with haematological malignancy that did not seem to be initiated by TF.
Factor XIII Val34Leu polymorphism and the risk of myocardial infarction under the age of 36 years
Authors: Politou Marianna , Komporozos Christoforos , Panagiotakos Demosthenes , Belessi Chrisoula , Travlou Anthi , Lekakis John , Kremastinos Dimitrios , Rallidis Loukianos 
Published: May 2008

SummaryThere are limited and controversial data regarding the impact of factor XIII (FXIII) Val34Leu polymorphism in the pathogenesis of premature myocardial infarction (MI). We examined whether FXIII Val34Leu polymorphism is associated with the development of early MI.We recruited 159 consecutive patients who had survived their first acute MI under the age of 36 years (mean age=32.1 ± 3.6 years, 138 were men). The control group consisted of 121 healthy individuals matched with cases for age and sex, without a family history of premature coronary heart disease (CHD). FXIII Val34Leu polymorphism was tested with polymerase chain reaction and reverse hybridization. There was a lower prevalence of carriers of the Leu34 allele in patients than in controls (30.2 vs. 47.1%, p=0.006). FXIII Val34Leu polymorphism was associated with lower risk for acute MI after adjusting for major cardiovascular risk factors (odds ratio [OR] = 0.51, 95% confidence interval [CI] 0.27–0.95, p=0.03). Subgroup analysis according to angiographic findings (“normal” coronary arteries [n=29] or significant CHD [n=130]) showed that only patients with MI and significant CHD had lower prevalence of carriers of the Leu34 allele compared to controls after adjusting for major cardiovascular risk factors (OR = 0.42, 95% CI 0.22–0.83, p=0.01). Our data indicate that FXIII Val34Leu polymorphism has a protective effect against the development of MI under the age of 36 years, particularly in the setting of significant CHD.
Cortical Activation Patterns during Long-term Memory Retrieval of Visually or Haptically Encoded Objects and Locations
Authors: Stock Oliver , Röder Brigitte , Burke Michael , Bien Siegfried , Rösler Frank 
Published: May 2008

Abstract
The present study used functional magnetic resonance imaging to delineate cortical networks that are activated when objects or spatial locations encoded either visually (visual encoding group, n = 10) or haptically (haptic encoding group, n = 10) had to be retrieved from long-term memory. Participants learned associations between auditorily presented words and either meaningless objects or locations in a 3-D space. During the retrieval phase one day later, participants had to decide whether two auditorily presented words shared an association with a common object or location. Thus, perceptual stimulation during retrieval was always equivalent, whereas either visually or haptically encoded object or location associations had to be reactivated. Moreover, the number of associations fanning out from each word varied systematically, enabling a parametric increase of the number of reactivated representations. Recall of visual objects predominantly activated the left superior frontal gyrus and the intraparietal cortex, whereas visually learned locations activated the superior parietal cortex of both hemispheres. Retrieval of haptically encoded material activated the left medial frontal gyrus and the intraparietal cortex in the object condition, and the bilateral superior parietal cortex in the location condition. A direct test for modality-specific effects showed that visually encoded material activated more vision-related areas (BA 18/19) and haptically encoded material more motor and somatosensory-related areas. A conjunction analysis identified supramodal and material-unspecific activations within the medial and superior frontal gyrus and the superior parietal lobe including the intraparietal sulcus. These activation patterns strongly support the idea that code-specific representations are consolidated and reactivated within anatomically distributed cell assemblies that comprise sensory and motor processing systems.
On-line Orthographic Influences on Spoken Language in a Semantic Task
Authors: Pattamadilok Chotiga , Perre Laetitia , Dufau Stéphane , Ziegler Johannes C. 
Published: May 2008

Abstract
Literacy changes the way the brain processes spoken language. Most psycholinguists believe that orthographic effects on spoken language are either strategic or restricted to meta-phonological tasks. We used event-related brain potentials (ERPs) to investigate the locus and the time course of orthographic effects on spoken word recognition in a semantic task. Participants were asked to decide whether a given word belonged to a semantic category (body parts). On no-go trials, words were presented that were either orthographically consistent or inconsistent. Orthographic inconsistency (i.e., multiple spellings of the same phonology) could occur either in the first or the second syllable. The ERP data showed a clear orthographic consistency effect that preceded lexical access and semantic effects. Moreover, the onset of the orthographic consistency effect was time-locked to the arrival of the inconsistency in a spoken word, which suggests that orthography influences spoken language in a time-dependent manner. The present data join recent evidence from brain imaging showing orthographic activation in spoken language tasks. Our results extend those findings by showing that orthographic activation occurs early and affects spoken word recognition in a semantic task that does not require the explicit processing of orthographic or phonological structure.
Evaluation of a psycho-educational group programme for family caregivers in home-based palliative care
Authors: Hudson P , Quinn K , Kristjanson L , Thomas T , Braithwaite M , Fisher J , Cockayne M 
Published: May 2008

Background: Family caregivers are often responsible for providing significant support to relatives who require palliative care at home. However, evidence suggests that family caregivers have limited information, resources or support to prepare them for such a role. Furthermore, family caregiving can be associated with negative physical, financial and psychosocial outcomes. Purpose: This project sought to examine the utility of a group family caregiver psycho-educational programme focused on preparing primary family caregivers for the role of supporting a relative with advanced cancer at home. Method: The education programme consisted of three consecutive weekly sessions presented in a group format, conducted at six home-based palliative care services across metropolitan and regional Victoria (Australia). Participating caregivers were required to complete a set of self-report questionnaires measuring caregiver competence, preparedness, optimism, rewards, social support, burden and information needs, at three time points: commencement of the programme (T1), upon completion (T2) and 2 weeks later (T3). Caregivers were also asked to report on the relevance, acceptability and content of the programme, as well as any barriers to access. Results: Sixteen education programmes were conducted, with 74 caregivers attending the first session. Forty-four caregivers completed all three data collection sets. Following the intervention, a significant positive effect was found for the following outcomes: preparedness for the caring role, caregiving competence, caregiving rewards and having information needs met from T1 to T2. These improvements were maintained at follow-up (T3). Feedback on the individual sessions and entire programme was favourable and the overwhelming majority of participants reported that the programme had a positive impact on their lives. Conclusions: This study demonstrated that a group education programme to prepare family caregivers for the role of supporting a dying relative at home was accessible, applicable and effective.
Cultural Transfer and National Identity in French Laicity
Authors: Baubérot Jean 
Published: May 2008

AbstractThis article examines the development of the concept of laicity and its specific application within the French political, social and cultural context. In doing so it contends that, far from being a ‘French exception’ as is sometimes perceived in the media, laicity in France drew on concepts and practices already in place in other countries at the time of the 1905 legislation separating church and state. The article concludes by asserting a distinction between laicity and secularism, whereby the former emphasises the separation of the roles of state and religion within a nation, whereas the latter refers to the progressive loss of relevance of religion within a culture. The specific definition of laicity has been enshrined in a recent International Declaration of Laicity in the 21st Century.
The Spread of Alphabetic Scripts (c. 1700—500 BCE)
Authors: Lemaire André 
Published: May 2008

AbstractThis article considers the origins of alphabetic writing, tracing its probable source to ancient Egypt, southern Levant or the Sinai during the Egyptian Middle Kingdom (17th century BCE). It supports the view that the earliest scripts were acrophonic representations of a West-Semitic language, whose use developed under the rule of the Hyksos in Egypt but was arrested there with the expulsion of this foreign dynasty at the end of the 16th century BCE. The development is then traced through the Levant, with particular attention given to the emergence of cuneiform alphabetic scripts in Ugarit (c. 1300 BCE). This form of writing disappeared with the fall of Ugarit, but linear alphabetic scripts were preserved in a variety of Near-Eastern languages, notably Aramaic and Phoenician. These two languages, by becoming linguae francae respectively of the Syria-northern Mesopotamia and the Anatolia-Eastern Mediterranean regions, brought about the spread of alphabetic writing up until the 8th century BCE. The article concludes by examining the influence of the Phoenician script on Greek, Etruscan and ultimately Latin forms of writing.
Melusine and Toyotamahime: Dissemination of a Culture by Sea
Authors: Shinoda Chiwaki 
Published: May 2008

AbstractJapan's founding myth tells the story of a crocodile princess called Toyotamahime, wife of Prince Hoori. She asks her husband not to look at her as she gives birth, but he disobeys her and so sees that his wife is a crocodile. Finding out that she has been seen, the princess goes back to her country, that is, the bottom of the sea. The tale can be compared to the one about Melusine: in both stories there are common motifs such as the command not to look and disobeying the command, as well as the transformation into a reptile. Both stories are also tales about the founding of a royal house. This paper shows how Japanese myths, closely connected to maritime traditions, are at the crossroad of a cultural circulation extending from China and Korea to Indonesia, India, Arabia and Egypt. The chief actors in these stories are monkeys, hares, crocodiles or turtles.