bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of pheromone response inSesamia nonagrioidesby the pheromone of the sympatric corn borer,Ostrinia nubilalis
Authors: Eizaguirre Matilde , Albajes Ramon , López Carmen , Sans Albert , Gemeno César 
Published: May 2007

AbstractSesamia nonagrioidesLef. andOstrinia nubilalis(Hübn.) are two corn borers present in the Mediterranean area that share a similar feeding habitat. The female sex pheromones of the two species consist of (Z)‐11‐hexadecenyl acetate, (Z)‐11‐hexadecen‐1‐ol, (Z)‐11‐hexadecenal and dodecyl acetate (77:8:10:5 w/w) and (Z)‐11‐tetradecenyl acetate and (E)‐11‐tetradecenyl acetate (97:3 w/w) respectively. Although the pheromones share no common components, previous work had shown the inhibition of the response byO. nubilalismales to their own pheromone owing to the presence ofS. nonagrioidespheromone. In this study, the pheromone ofO. nubilalisand its two components separately were shown to inhibit the attraction ofS. nonagrioidesmales to the synthetic female pheromone in both laboratory wind‐tunnel bioassay and field trapping studies. In the wind tunnel, the number of contacts ofS. nonagrioidesmales with the source were significantly (P< 0.05) reduced by the single pheromone components ofO. nubilalis. In the field, the addition of 1% ofO. nubilalispheromone significantly (P< 0.05) reduced the catches ofS. nonagrioidesmales. The components of both pheromones also elicited electroantennographic responses from antennae of maleS. nonagrioidesmoths. The ecological consequences and the possibilities of applying this cross‐inhibition for mating disruption techniques are discussed. Copyright © 2007 Society of Chemical Industry
Resistance to spinosad in the western flower thrips, Frankliniella occidentalis (Pergande), in greenhouses of south‐eastern Spain
Authors: Bielza Pablo , Quinto Vicente , Contreras Josefina , Torné María , Martín Alberto , Espinosa Pedro J 
Published: May 2007

AbstractSusceptibility to spinosad of western flower thrips (WFT), Frankliniella occidentalis (Pergande), from south‐eastern Spain was determined. LC50 values of the field populations without previous exposure to spinosad collected in Murcia in 2001 and 2002 ranged from 0.005 to 0.077 mg L−1. The populations collected in Almeria in 2003 in greenhouses were resistant to spinosad (LC50 > 54 mg L−1) compared with the authors' highly susceptible laboratory strain. The highly sensitive laboratory strain leads to very high resistance ratios for the field populations (>13 500), but these ratios do not necessarily mean resistance problems and control failures (spinosad field rate 90–120 mg L−1). The populations collected in Murcia from some greenhouses in 2004 were also resistant to spinosad (RF > 3682). Spinosad overuse, with more than ten applications per crop, produced these resistant populations in some greenhouses. Spinosad showed no cross‐resistance to acrinathrin, formetanate or methiocarb in laboratory strains selected for resistance towards each insecticide. Correlation analysis indicated no cross‐resistance among spinosad and the other three insecticides in 13 field populations and in nine laboratory strains. The synergists piperonyl butoxide (PBO), S,S,S‐tributyl phosphorotrithioate (DEF) and diethyl maleate (DEM) did not enhance the toxicity of spinosad to the resistant strains, indicating that metabolic‐mediated detoxification was not responsible for the spinosad resistance. These findings suggest that rotation with spinosad may be an effective resistance management strategy. Copyright © 2007 Society of Chemical Industry
Comparison of the subchronic antidiabetic effects of DPP IV–resistant GIP and GLP‐1 analogues in obese diabetic (ob/ob) mice
Authors: Irwin Nigel , McClean Paula L. , Flatt Peter R. 
Published: May 2007

AbstractGlucagon‐like peptide‐1 (GLP‐1) and glucose‐dependent insulinotropic polypeptide (GIP) are the two key incretin hormones released from the gastrointestinal tract that regulate blood glucose homeostasis through potent insulin secretion. The rapid degradation of GIP and GLP‐1 by the ubiquitous enzyme dipeptidyl peptidase IV (DPP IV) renders both peptides noninsulinotropic. However, DPP IV stable agonists, such as N‐AcGIP and (Val8)GLP‐1, have now been developed. The present study has examined and compared the metabolic effects of subchronic administration of daily i.p. injections of N‐AcGIP, (Val8) GLP‐1 and a combination of both peptides (all at 25 nmol/kg bw) in obese diabetic (ob/ob) mice. Initial in vitro experiments confirmed the potent insulinotropic properties of N‐AcGIP and (Val8)GLP‐1 in the clonal pancreatic BRIN BD11 cell line. Subchronic administration of N‐AcGIP, (Val8)GLP‐1 or combined peptide administration had no significant effects on the body weight, food intake and plasma insulin concentrations. However, all treatment groups had significantly (p < 0.05) decreased plasma glucose levels and improved glucose tolerance by day 14. The effectiveness of the peptide groups was similar, and glucose concentrations were substantially reduced following injection of insulin to assess insulin sensitivity compared to control. These results provide evidence for an improvement of glucose homeostasis following treatment with enzyme‐resistant GIP and GLP‐1 analogues. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
Establishment and clinical application of enzyme immunoassays for determination of luteinizing hormone releasing hormone and metastin
Authors: Katagiri Fumihiko , Tomita Kenji , Oishi Shinya , Takeyama Masaharu , Fujii Nobutaka 
Published: May 2007

AbstractMetastin, a 54‐residue peptide, was identified as the cognate ligand of human G‐protein‐coupled receptor GPR54. Since metastin is a gene product of the human metastasis suppressor gene ‘KiSS‐1’, early studies on metastin were focused on its activity as a tumor metastasis suppressor. Recently, there have been some reports that metastin is found in human plasma and is particularly abundant in the plasma of pregnant women. Dysfunction of the GPR54 receptor causes diseases that are characterized by an insufficient release of gonadotropin and lack or delay of pubertal maturation. This information strongly suggests that metastin is involved in the regulation of reproductive endocrine functions. In order to determine the plasma levels of metastin and luteinizing hormone releasing hormone (LHRH) in an isolated hypogonadotropic hypogonadism (IHH) patient, who received intermittent administrations of LHRH, we tried to establish a sensitive and specific enzyme immunoassay. The plasma LHRH levels of the patient were very high, while plasma metastin levels were at almost the same levels as circadian rhythms of healthy male humans. In the central nervous system, metastin stimulates the neuroendocrine reproductive axis. However, the effects of peripheral metastin are not known. Our result suggested that peripheral metastin had a genesis and activity different from central metastin. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
Effects of dietary red clover on blood factors and cardiovascular fatty streak formation in hypercholesterolemic rabbits
Authors: Asgary S. , Moshtaghian J. , Naderi G. , Fatahi Z. , Hosseini M. , Dashti G. , Adibi S. 
Published: May 2007

AbstractPhytoestrogens with a biological activity like estradiol are naturally found in many plants. This study was designed to investigate the effect of red clover, a phytoestrogen‐rich member of the legume family (Trifolium pratense) on the development of atherosclerosis in male hyperlipidemic rabbits. Twenty rabbits were semi‐randomly distributed into four groups of five each. Two groups received either normal diet or normal diet supplemented with red clover. Two other groups received similar diets to both of which 1% cholesterol was added. Dietary use of red clover (RC) in hyperlipidemic rabbits significantly decreased C‐reactive protein (CRP), triglyceride (TG), total cholesterol and LDL‐cholesterol (LDL‐C) whereas, HDL‐cholesterol (HDL‐C) was significantly increased in those animals (p < 0.05). Fatty streak formation was also significantly lower in aorta and left and right coronary arteries in the same animals due to use of dietary RC supplementation. These findings suggest that dietary RC may reduce cardiovascular risk factors. Copyright © 2007 John Wiley & Sons, Ltd.
Treating primary infertility due to pituitary atrophy with Chinese herbs: a case report
Authors: Zhou Jue , Qu Fan 
Published: May 2007

AbstractIn the report a patient who suffered from secondary amenorrhea for 6 years and primary infertility for 2 years due to pituitary atrophy was successfully cured with Chinese herbs. After orally administered Chinese herbs for 1 month, the patient menstruated once and became pregnant later. Copyright © 2007 John Wiley & Sons, Ltd.
Effects of propolis extract on sensitivity to chemotherapeutic agents in HeLa and resistant sublines
Authors: Takara Kohji , Fujita Megumi , Matsubara Mika , Minegaki Tetsuya , Kitada Noriaki , Ohnishi Noriaki , Yokoyama Teruyoshi 
Published: May 2007

AbstractThe effects of a propolis extract obtained by supercritical fluid extraction on sensitivity to chemotherapeutic agents were examined in HeLa cells and resistant sublines thereof. In addition, the actions of propolis and caffeic acid phenethyl ester (CAPE), a constituent of propolis, on the multidrug efflux transporter P‐glycoprotein/MDR1, were evaluated in paclitaxel‐resistant HeLa/TXL cells (MDR1‐overexpressing cells). In HeLa cells, the sensitivity to paclitaxel and doxorubicin, substrates of MDR1, was unchanged in the presence of propolis. In HeLa/TXL cells, propolis increased sensitivity to these MDR1 substrates. The accumulation of Rhodamine123, also a substrate for MDR1, by HeLa/TXL cells increased in the presence of 50 µg/mL, but not 10 µg/mL, of the extract. However, the growth inhibition of HeLa/TXL cells by paclitaxel was not changed by CAPE, although the accumulation of Rhodamine123 increased significantly in the presence of 100 µm, but not 1 nM or 1 µm, CAPE. Collectively, the extract was suggested to inhibit the function of MDR1 and to increase the sensitivity to MDR1 substrates in HeLa/TXL cells, effects likely to be caused by constituents other than CAPE. Copyright © 2007 John Wiley & Sons, Ltd.
Effect of acute administration of hydroalcohol extract of Ilex paraguariensis St Hilaire (Aquifoliaceae) in animal models of Parkinson's disease
Authors: Milioli E. M. , Cologni P. , Santos C. C. , Marcos T. D. , Yunes V. M. , Fernandes M. S. , Schoenfelder T. , Costa‐Campos L. 
Published: May 2007

AbstractIlex paraguariensis St Hilaire (Aquifoliaceae) is a plant widely cultivated in South America and with various reputed medicinal properties that can be attributed to phenolic constituents of the leaves: caffeine, theophylline and theobromine, besides the flavonoids, quercetin and rutin. This study examined the antiparkinsonian activity of the hydroalcohol extract of Ilex paraguariensis in models of protection against cerebral injury induced by MPTP and reversal of the catatonia induced by reserpine in mice. The hydroalcohol extract prevented MPTP‐induced hypolocomotion at doses of 250 and 500 mg/kg at the all time points observed and also prevented the reserpine‐induced catalepsy at the same doses. The extract potentiated the effect of apomorphine in preventing catatonia, suggesting a non‐dopaminergic activity, probably through antagonism of adenosine. In biochemical studies the hydroalcohol extract caused a significant decrease in the NO levels, exhibited a DPPH‐scavenging ability and was effective in preventing the oxidation of deoxyribose. The results obtained suggest that the hydroalcohol extract of Ilex paraguariensis may have an antiparkinsonian profile in animal models, probably through its antioxidant activity and antagonist action on adenosine A2A receptors. Copyright © 2007 John Wiley & Sons, Ltd.
A randomized, double blind, placebo‐controlled, prospective clinical study to demonstrate clinical efficacy of a fixed valerian hops extract combination (Ze 91019) in patients suffering from non‐organic sleep disorder
Authors: Koetter U. , Schrader E. , Käufeler R. , Brattström A. 
Published: May 2007

AbstractValerian and hops are traditionally used as sleep aids. Since the fixed extract combination (Ze 91019) as a whole is considered the active compound, the clinical efficacy must be demonstrated for this extract combination. The present clinical study aimed to demonstrate superiority of the fixed extract combination in comparison with placebo in patients suffering from non‐organic insomnia (ICD 10, F 51.0–51.2). Objective sleep parameters were registered by means of a transportable home recorder system (QUISI). The primary outcome was the reduction in sleep latency (SL2) which had to be prolonged at baseline (≥30 min) as an inclusion criteria. The treatment period lasted for 4 weeks with either placebo, single valerian extract (Ze 911) or the fixed valerian hops extract combination (Ze 91019). The amount of the single valerian extract was identical to that amount contained in the fixed extract combination, i.e. 500 mg valerian extract siccum. In the extract combination 120 mg hops extract siccum was added. Both the extracts were prepared with 45% methanol m/m with a drug–extract ratio of 5.3:1 (valerian) and 6.6:1 (hops), respectively. The fixed extract combination was significantly superior to the placebo in reducing the sleep latency whilst the single valerian extract failed to be superior to the placebo. The result underlined the plausibility for adding hops extract to the valerian extract. Copyright © 2007 John Wiley & Sons, Ltd.
Antimalarial activity of some plants traditionally used in Meru district of Kenya
Authors: Muthaura C. N. , Rukunga G. M. , Chhabra S. C. , Omar S. A. , Guantai A. N. , Gathirwa J. W. , Tolo F. M. , Mwitari P. G. , Keter L. K. , Kirira P. G. , Kimani C. W. , Mungai G. M. , Njagi E. N. M. 
Published: May 2007

AbstractTen plant extracts commonly used by the Meru community of Kenya were evaluated for thein vitroantiplasmodial,in vivoantimalarial, cytotoxicity and animal toxicity activities. The water and methanol extracts ofLudwigia erectaand the methanol extracts ofFuerstia africanaandSchkuhria pinnataexhibited high antiplasmodial activity (IC50< 5 µg/mL) against chloroquine sensitive (D6) and resistant (W2)Plasmodium falciparumclones. The cytotoxicity of these highly active extracts on Vero E6 cells were in the range 161.5–4650.0 µg/mL with a selectivity index (SI) of 124.2–3530.7.In vivostudies of these extracts showed less activity with chemosuppression of parasitaemia inPlasmodium bergheiinfected mice of 49.64–65.28%. The methanol extract ofClerodendrum eriophyllumwith a lowerin vitroactivity (IC509.51–10.56 µg/mL) exhibited the highest chemosuppression of 90.13%. The methanol and water extracts ofPittosporum viridiflorumwere toxic to mice but at a lower dose prolonged survival ofP. bergheiinfected mice (p< 0.05) with no overt signs of toxicity. However, the extracts were cytotoxic (SI, 0.96–2.51) on Vero E6 cells. These results suggest that there is potential to isolate active non‐toxic antimalarial principles from these plants. Copyright © 2007 John Wiley & Sons, Ltd.
Toki‐to protects dopaminergic neurons in the substantia nigra from neurotoxicity of MPTP in mice
Authors: Sakai Risa , Irie Yoshifumi , Murata Takeshi , Ishige Atsushi , Anjiki Naoko , Watanabe Kenji 
Published: May 2007

AbstractParkinson's disease (PD) is a neurodegenerative disease of the brain characterized by the progressive loss of dopaminergic neurons in the substantia nigra (SN). No clinically proven drugs that may halt or retard the progression of PD have been reported. This study examined the anti‐PD effect of a traditional Japanese/Chinese herbal remedy Toki‐to (TKT) using mice treated with a neurotoxin 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydroxypyridine (MPTP). TKT showed improvement of MPTP‐induced PD‐like symptoms (bradykinesia) in a behavioral test (pole test). Histological studies of SNs from these mice demonstrated that TKT had a protective effect on dopaminergic neurons against MPTP neurotoxicity. Real‐time RT‐PCR analyses of mRNA from SNs demonstrated that expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) genes were decreased by MPTP treatment and that these decreases were reversed by TKT administration prior to MPTP treatment. DNA microarray analyses indicated that TKT per se suppressed gene expression of serum‐ and glucocorticoid regulated kinase (SGK) that is believed to be a molecule that drives the pathogenesis of PD. Hence, it is suggested that TKT may inhibit the activation of SGK at the transcriptional level and thusmay participate in halting the progression of MPTP‐induced neurotoxicity. Copyright © 2007 John Wiley & Sons, Ltd.
The universal Hamiltonian of the exchange interaction for the system of particles with an arbitrary spin j
Authors: Orlenko Elena 
Published: May 2007

AbstractOn the basis of the arrangement symmetry for the system of identical particles the universal Hamiltonian of the exchange interaction in the spin representation is developed. For the case of spin‐1/2 particles the obtained Hamiltonian is equivalent to the Heisenberg form. © 2007 Wiley Periodicals, Inc. Int J Quantum Chem, 2007
Identification and determination of fat‐soluble vitamins and metabolites in human serum by liquid chromatography/triple quadrupole mass spectrometry with multiple reaction monitoring
Authors: Priego Capote Feliciano , Jiménez José Ruiz , Granados José María Mata , de Castro María Dolores Luque 
Published: May 2007

AbstractA method for determination of fat‐soluble vitamins K1, K3, A, D2, D3 and E (as α‐ and δ‐tocopherol) and metabolites 25‐hydroxyvitamin D2 and D3 and 1,25‐dihydroxyvitamin D3 in human serum by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) in positive mode is proposed. Highly selective identification of the target compounds in serum was confirmed by the most representative transitions from precursor ion to product ion. Quantitative MS/MS analysis was carried out by multiple reaction monitoring optimizing the most sensitive transition for each analyte in order to achieve low detection limits (from 0.012 to 0.3 ng/mL estimated with serum). The analysis was performed with 1 mL of serum, which was subjected to protein precipitation, liquid–liquid extraction to an organic phase, evaporation to dryness and reconstitution with methanol. The precision of the overall method ranged from 3.17–6.76% as intra‐day variability and from 5.07–11.53% as inter‐day variability. The method, validated by the standard addition method, provides complete information on the fat‐soluble vitamins profile, which is of interest in clinical and metabolomics studies. Copyright © 2007 John Wiley & Sons, Ltd.
Study of the coupling reaction of pyridine in the gas phase
Authors: Yao Shengjun , Zhang Xiang , Zhang Lei , Wang Cuihong , Guo Yinlong 
Published: May 2007

AbstractThe coupling reaction of pyridine in the gas phase to form bipyridyl and terpyridyl has been studied by electron ionization using an ion trap mass spectrometer. In contrast to the difficulty in carrying out electrophilic substitutions at carbon atoms in the pyridine ring under highly acidic solvent conditions, reactions in the gas phase overcame the conjugate acidification of pyridine in the solvent phase, thus decreasing the hardness of this electrophilic coupling. Through product ion mass spectra of the ion at m/z 157, we have shown that this ion was protonated bipyridyl rather than the ion/molecule adduct. A computational study of the heat of formation surface also supported the formation of polypyridyls through the electrophilic substitution of pyridine. We have confirmed the reaction through a study of pyridine‐d5 coupling in the gas phase. Copyright © 2007 John Wiley & Sons, Ltd.
Highly sensitive assay for tiotropium, a quaternary ammonium, in human plasma by high‐performance liquid chromatography/tandem mass spectrometry
Authors: Wang Jiang , Jiang Yao , Wang Yingwu , Li Hao , Fawcett J. Paul , Gu Jingkai 
Published: May 2007

AbstractTiotropium bromide, a long‐acting inhaled bronchodilator analogous to ipratropium bromide, is currently undergoing development for the treatment of chronic obstructive pulmonary disease. To evaluate its systemic absorption in humans, we have developed a rapid and sensitive method for its determination in human plasma based on high‐performance liquid chromatography with tandem mass spectrometric detection (HPLC/MS/MS). Reversed‐phase chromatography of tiotropium and the internal standard clenbuterol was carried out using acetonitrile/10 mM ammonium acetate (1% formic acid) 40:60 as mobile phase in a run time of 3.0 min. The sample preparation involved deproteination with acetonitrile, extraction into dichloromethane and back‐extraction into hydrochloric acid. The assay was linear over the concentration range 0.500–50.0 pg/mL with intra‐ and inter‐day precision (as relative standard deviation) both ≤7.34%. The method was successfully applied to a pharmacokinetic study of systemic absorption in healthy male volunteers given a single 18 µg inhaled dose. Copyright © 2007 John Wiley & Sons, Ltd.
Deuterium and oxygen‐18 determination of microliter quantities of a water sample using an automated equilibrator
Authors: Uemura Ryu , Matsui Yohei , Motoyama Hideaki , Yoshida Naohiro 
Published: May 2007

AbstractWe describe a modified version of the equilibration method and a correction algorithm for isotope ratio measurements of small quantities of water samples. The δD and the δ18O of the same water sample can both be analyzed using an automated equilibrator with sample sizes as small as 50 µL. Conventional equilibration techniques generally require water samples of several µL. That limitation is attributable mainly to changes in the isotope ratio (18O/16O or D/H) of water samples during isotopic exchange between the equilibration gas (CO2 or H2) and water, and therefore the technique for µL quantities of water requires mass‐balance correction using the water/gas (CO2 or H2) mole ratio to correct this isotopic effect. We quantitatively evaluate factors controlling the variability of the isotopic effect due to sample size. Theoretical consideration shows that a simple linear equation corrects for the effects without determining parameters such as isotope fractionation factors and water/gas mole ratios. Precisions (1‐sigma) of 50‐µL meteoric water samples whose isotopic compositions of −1.4 to −396.2‰ for δD are ±0.5 to ±0.6‰, and of −0.37 to −51.37‰ for δ18O are ±0.01 to ±0.11‰. Copyright © 2007 John Wiley & Sons, Ltd.
Water–polysaccharide macromolecules: competitive interaction on glass microspheres
Authors: Piccirilli P. , Caracciolo N. , Enrich C. , Boeykens S. , Vázquez C. , Rosen M. 
Published: May 2007

AbstractThe equilibrium properties of a polymer solution in the vicinity of a solid–liquid interface are locally altered relative to those in bulk. Discrepancies between porous media and conventional rheology appear when the pore wall is either attractive or repulsive for the polymer adsorption. These local alterations become evident in practical situations, such as filtration, dissolution, purification, chromatography of polymers and various membrane permeation situations, as well as in many industrial applications.In the present paper we study the adsorption of scleroglucan on a glass surface. In order to understand polymer adsorption at a liquid–solid interface, it is necessary to consider the competitive interaction that takes place between the macromolecules and the solvent molecules on the solid surface and the structural transformations of the system as a whole. The experiments were performed with aqueous scleroglucan solutions of concentrations up to 0.1% on 400–600 µm glass microspheres. The polymer had been previously labeled with iodine in a chemical reaction. The iodine concentration was detected by total reflection x‐ray fluorescence spectrometry (TXRF). The adsorbed polymer films were evaluated as given by the concentration measurements. Copyright © 2007 John Wiley & Sons, Ltd.