bluuebunny/crossref_metadata_2025_split huggingface dataset:
Person-to-Person Microbiota Transmission Can Influence Depression and Anxiety in Newly Married Couples: Six-Month Interim Results
Authors: Rastmanesh Reza , Vellingiri Balachandar , Isacco Ciro Gargiulo , Sadeghinejad Abolfazl , Daghnall Neil 
Published: February 2025

ABSTRACTBackgroundOral microbiota dysbiosis and salivary cortisol are associated with depression and anxiety. Bacterial transmission can occur between spouses.AimsWe explored whether oral microbiota, salivary cortisol and a combined depression-anxiety (DA) phenotype affiliated in newly married couples.MethodsThe researchers administered validated Persian versions of the Pittsburgh Sleep Quality Inventory (PSQI), Beck Depression Inventory-II (BDI-II), and Beck Anxiety Inventory to 1740 couples, who had been married during the past six months. The investigators compared 296 healthy control spouses with 296 cases. Data analysis used appropriate statistical methods.ResultsAfter six months, at the phyla level, we identified a significant decrease in Firmicutes and Actinomycetota abundance and an increase in Bacteroidetes, Proteobacteria, Fusobacteria and Patescibacteria abundance in healthy spouses married to an insomniac with DA-phenotype, showing that oral microbiota were significantly changed and became similar to that of participant’s spouse, (i.e., if the spouse had DA-phenotype, then composition of oral microbiota became similar to their spouse DA-phenotype,p<0.001). These changes parallelled alterations in salivary cortisol, depression and anxiety scores. Linear discriminant analysis (LDA) showed that relative abundances of Clostridia, Veillonella, Bacillus and Lachnospiraceae were significantly higher in insomniacs with DA-phenotype than healthy controls (p<0.001). Results remained significant after controlling for confounders. The formal mediation analysis confirmed these outcomes. We observed distinct analogous gender differences for oral microbiota pattern, salivary cortisol level, and depression and anxiety scores.ConclusionsMicrobiota transamination between two people in close contact with one another partially mediated depression and anxiety.
Complex nitrogen redox couplings control methane emissions from Arctic upland yedoma taliks
Authors: Bergman Oded , Anthony Katey Walter , Eliani-Russak E. , Sivan Orit 
Published: February 2025

AbstractYedoma-permafrost holds disproportionately large carbon and nitrogen pools, concentrated in icy, Pleistocene-aged silt deposits in the Arctic. Upon thaw, these undergo microbial mineralization, releasing greenhouse gases (GHGs) including carbon-dioxide (CO2), methane (CH4) and nitrous-oxide (N2O). Here we present combined geochemical data with microbial function and community dynamics from deep-talik soil boreholes in an unsaturated yedoma upland. Our results reveal significant in-situ spatio-temporal seasonal shifts in microbial functional, community composition and diversity within 7-m deep upland talik. In situ methanogenesis persisted in the soil talik throughout the year due to the permafrost thaw. In the winter methanotrophy was negligible within and above the methanogenic zone, leading to elevated CH4emissions to the atmosphere. This is likely due to reduced microbial methanotrophic activity, associated with lower temperatures and nitrogen availability. During summer, at and above the anoxic methanogenic zone, nitrate/nitrite mediated anaerobic oxidation of methane (N-AOM) by ANME2d and the NC-10 phylum, together with aerobic methanotrophy near the soil surface, significantly attenuated CH4emissions. Nitrous-oxide concentrations peaked at 10 cm (7.2 µM) and 105 cm (6.7 µM) and were associated with denitrification and N-AOM byMethanoperedens(ANME2d). In the summer only and within the top 1 m of soil, high expression of nitrogen related genes (narG, norB, amoA, Annamox, and Feammox) indicated active redox dynamics, potentially providing nitrogen species for N-AOM. The potential N2O emissions in summer may imply higher net GHGs emission from yedoma uplands as climate warming leads to longer summers and warmer soils in the future.
Exploiting the SunTag system to study the developmental regulation of mRNA translation
Authors: Pizzey Alastair , Sutcliffe Catherine , Love Jennifer C. , Akabuogu Emmanuel , Rattray Magnus , Ashe Mark P. , Ashe Hilary L. 
Published: February 2025

AbstractThe ability to quantitatively study mRNA translation using SunTag imaging is transforming our understanding of the translation process. Here, we expand the SunTag method to study new aspects of translation regulation inDrosophila. Repression of the maternalhunchback(hb) mRNA in the posterior of theDrosophilaembryo is a textbook example of translational control. Using SunTag imaging to quantitate translation of maternalSunTag-hbmRNAs, we show that repression in the posterior is leaky as ∼5% ofSunTag-hbmRNAs are translated. In the anterior of the embryo, the maternal and zygoticSunTag-hbmRNAs show similar translation efficiency despite having different UTRs. We demonstrate that theSunTag-hbmRNA can be used as a reporter to study ribosome pausing at single-mRNA resolution, by exploiting the conservedxbp1mRNA and A60 pausing sequences. Finally, we adapt the detector component of the SunTag system to visualise and quantitate translation of theshort gastrulation(sog) mRNA, encoding an essential secreted extracellular BMP regulator, at the endoplasmic reticulum in fixed and live embryos. Together, these tools will facilitate the future dissection of translation regulatory mechanisms during development.
Standardized droplet preamplification method for downstream circulating cell-free DNA analysis
Authors: Skeen Colin , Pratt Erica D. 
Published: February 2025

AbstractCirculating cell-free DNA (ccfDNA) can be found in blood and other biofluids and is a minimally invasive biomarker for several pathological processes. As tumors become more invasive, an increasing amount of circulating tumor DNA (ctDNA) is also shed into the peripheral circulation. Combined analysis of ccfDNA and ctDNA has demonstrated prognostic and predictive value in metastatic disease. However, localized tumors shed significantly less ccfDNA/ctDNA and accurate detection remains a technical challenge. To overcome this barrier, droplet preamplification has been used to perform robust multiplexed analysis of low input samples. To reduce false positives, it is essential to use a high-fidelity polymerase with 3’-5’ exonuclease activity. However, attempts to combine high-fidelity polymerases with commercial droplet digital chemistries have had limited success. There is also no standardized method for efficient amplicon recovery from droplets. In this work, we present a method to reliably stabilize emulsions and recover preamplified template. We systematically compared our protocol with different destabilization methods and found an average 41% improvement in recovery efficiency. We anticipate this standardized method will increase the consistency and reproducibility of ccfDNA/ctDNA analyses. This technique could be readily translated to other low-input or low-biomass samples, such as urine, saliva, or archived biopsy specimens.
A hybrid forecasting model to improve cost prediction accuracy in green building projects with machine learning
Authors: Wu Zhijiang , Liu Mengyao , Ma Guofeng , Jiang Shan 
Published: February 2025

PurposeThe objective of this study is to accurately predict the cost of green buildings to provide quantifiable criteria for investment decisions from investors.Design/methodology/approachThis study proposes a hybrid prediction model ML-based for cost prediction of GBPs and obtains prediction parameters (PPs) associated with project characteristics through data mining (DM) techniques. The model integrates a principal component analysis (PCA) method to perform parameter dimensionality reduction (PDR) on a large number of raw variables to provide independent characteristic terms. Moreover, the support vector machine (SVM) algorithm is improved to optimize the prediction results and integrated with parameter dimensionality reduction and cost prediction.FindingsThe prediction results show that the mean absolute and relative errors of the hybrid prediction model proposed in this study are equal to 39.78 and 0.02, respectively, which are much lower than those of the traditional SVM model and MRA prediction model. Moreover, the hybrid prediction model with parameter dimensionality reduction also achieved better prediction accuracy (R2 = 0.319) and superior prediction accuracy for different cost terms.Originality/valueTheoretically, the hybrid prediction model developed in this study can reliably predict the cost while accurately capturing the characteristics of GBPs, which is a bold attempt at a comprehensive approach. Practically, this study provides developers with a new ML-based prediction model that is capable of capturing the costs of projects with ambiguous definitions and complex characteristics.
Smart IoT-enabled vehicle-mounted weighing system for real-time monitoring and quantification of loose construction materials
Authors: Jadoon Abeer Ahmed , Aslam Mughees , Malik Muhammad Sohail Anwar , Alam Hamza 
Published: February 2025

PurposeIn the construction industry (CI), inaccurate loose material measurements pose waste and economic challenges, accounting for approximately one-third of project cost overruns, representing 60% of the overall construction costs. Current practices for measuring loose construction materials are riddled with errors, direct adjustments and missing real-time oversight, resulting in waste, inefficiencies and cost escalations. This study aims to address this issue by automating the weighing mechanism of loose materials while regularly monitoring them during transportation from the source.Design/methodology/approachIn this study, an innovative Internet of Things (IoT) solution: a smart vehicle-mounted weighing (VMW) system was developed using the smart sensors, Arduino while using C++ language. Initially, a prototype was developed and tested/ refined while experimenting on a Tractor trolley loaded with loose construction materials. Statistical methods were employed to test the validity of the developed system against traditional methods of weighing. Finally, the system was tested and optimized in a real project through a case study.FindingsThe empirical findings reveal that the IoT-enabled smart weighing system achieved an accuracy exceeding 95% during rigorous field assessments. A comprehensive case study highlighted significant cost savings, with 2.7% for subbase materials and 5.94% for aggregate base materials. These results validate the successful development of the prototype, demonstrating its potential to improve material management, minimize waste, and enhance operational efficiency in construction projects while promoting sustainable practices.Originality/valueFew studies have addressed fully automated measurement of loose materials during transport to construction sites. This study introduces a novel IoT-enabled smart VMW system for real-time material monitoring and automated quantity takeoffs (QTOs). The system offers CI a reliable, transparent, and error-free material management tool, advancing current practices in material quantification thereby leading the CI toward sustainability using the latest technologies.
Predicting microfinance inclusion and survival of microenterprises in rural Uganda: testing the mediating role of ethical financial behavior of poor young women owners
Authors: Okello Candiya Bongomin George , Semukono Frederick , Baleke Yiga Lubega Joseph , Balinda Rebecca 
Published: February 2025

Purpose
The main purpose of this study is to test whether ethical financial behavior as a mediator promotes microfinance inclusion and survival of the poor young women microenterprises in rural Uganda.

Design/methodology/approach
The methods recommended by Kenny et al. (1998); Shrout and Bolger (2002); MacKinnon et al. (2004); and Preacher and Hayes (2004) were used to establish the existence of non-zero monotonic association between microfinance inclusion and survival through testing the mediating effect of ethical financial behavior in SmartPLS.

Findings
The results from the structural equation modeling revealed a significant full mediating effect of ethical financial behavior in the relationship between microfinance inclusion and survival of the poor young women microenterprises. Microfinance inclusion and ethical financial behavior explain 62 % of the variation in survival of the poor young women microenterprises in rural Uganda.

Research limitations/implications
Whereas significant results were obtained from this study, the data were collected only from rural-based poor young women microenterprises located in northern Uganda. Extending the sample to cover the whole country may provide a more representative picture. Besides, it would be useful to compare results across developing countries as this may provide information about the generality of our findings.

Practical implications
The findings from this study can be useful to managers of microfinance institutions in developing countries to adopt practice that can promote financial discipline among rural poor young women microentrepreneurs. Routine financial education and business mentorship can be organized through workshops, trainings and seminars to teach rural poor young women microentrepreneurs how to manage money, especially business loans borrowed from the microfinance institutions to put it into right use. This can help them to meet timely loan repayment to increase access to future microfinance loans.

Originality/value
This study provides the first evidence on the use of the theory of planned behavior (TPB) and theory of reasoned action (TRA) to explain microfinance inclusion of the poor young women microentrepreneurs in rural Uganda. The study uses a blend of TPB and TRA derived from psychology and sociology to explain repayment intention and ethical behaviors of the poor young women borrowers, which determines the microfinance lending cycle to make microcredit available for them to engage in entrepreneurship to come out of poverty to attain wellbeing.
Design and analysis of a cable-driven continuum robot with used in situ inspection
Authors: Qi Fei , Ge Yiwei , Liu Xianjun 
Published: February 2025

Purpose
This paper aims to present a kinematics performance analysis and control for a continuum robot based on a dynamic model to achieve control of the robot.

Design/methodology/approach
To analyze the motion characteristics of the robot, its kinematics model is derived by the geometric analysis method, and the influence of the configuration parameters of the robot on workspace is investigated. Moreover, the dynamic model is established by the principle of virtual work to analyze the mapping relationship among the bending shape, the forces/torques applied to the robot. To achieve better control of the robot, a control strategy for continuum robot based on the dynamic model is put forward.

Findings
Results of the simulations and experiments verify the proposed continuum structure and motion model, the maximum position error is 5.36 mm when the robot performs planar bending motion and the average position error of the robot in spatial circular motion is 5.84 mm. The proposed model can accurately describe the deformation movement of the robot and realize its motion control with a few position errors.

Originality/value
The kinematics analysis and control model proposed in this paper can achieve precise control of the robot, which can be used as a reference for the motion planning and shape reconstruction of continuum robot.
Looking at the beauty of halal through the stimulus-organism-response model and gender perspectives: the case of Indonesian Muslim
Authors: Hati Sri Rahayu Hijrah , Fenitra Rakotoarisoa Maminirina , Masood Adlin , Setyowardhani Hapsari , Abdul Rahim Alina , Sumarwan Ujang 
Published: February 2025

Purpose
This study aims to explore the impact of external stimuli – perceived product quality, advertising value and the halal logo – on Muslim consumers’ attitudes and purchase intentions, using the stimulus–organism–response (S-O-R) model in accordance with its traditional framework. Additionally, it seeks to analyze and compare the purchasing behaviors of male and female consumers in the context of halal cosmetics.

Design/methodology/approach
An online survey using purposive sampling was conducted with 635 Muslim consumers in Indonesia to test the proposed model using structural equation modeling.

Findings
The results demonstrated that perceived quality, advertising value and the halal logo significantly enhance attitudes toward halal cosmetics. However, when it comes to purchase intention, only advertising value and the halal logo directly influence it. In terms of gender comparison, the study found significant moderation of gender in the relationship between advertising value and attitude, with this influence being more pronounced among male consumers. The findings indicate that both perceived quality and the halal logo significantly and positively impact attitudes and purchase intentions for both male and female Muslim consumers, with a notably stronger effect observed among females. Interestingly, the influence of advertising value on attitude is significant only among male consumers, highlighting a gender-specific response to advertising stimuli.

Practical implications
The study suggests that businesses operating in the halal cosmetics market should focus on enhancing consumers’ perception of product quality through halal certification. Additionally, they should tailor their marketing strategies based on gender differences and prioritize the prominent display of the halal logo. These actions are expected to positively influence the attitudes and purchase intentions of Muslim consumers in Indonesia.

Originality/value
This study introduces a novel approach to the understanding of consumer behavior toward halal cosmetics by emphasizing the frequently overlooked role of external influences through the S-O-R theory. In contrast to earlier research, which predominantly focused on internal factors and religious compliance, this study explores the impact of marketing interventions such as product quality, advertising and the presence of a halal logo. Additionally, it distinguishes itself by examining the purchasing behavior of both female and male consumers in the realm of halal cosmetics.
Possible complications in oral surgery and their management in patients affected by type 1 diabetes: narrative review
Authors: Nagni Matteo , Severino Marco , Redi Lorenzo , Zizza Agostino , Pancrazi Gian Luca , Vavassori Emilio , D'Orto Bianca 
Published: February 2025

Diabetes mellitus is a disease that affects millions of people worldwide, both in its juvenile form (type 1 diabetes) and in the form that affects adults and is related to lifestyle, diet, and sedentariness (type 2 diabetes). In addition to the disease itself, diabetes also includes various complications and, above all, creates a supportive environment for the development of further disorders and pathologies. Certain alterations favor oral pathologies in diabetic patients.
This narrative review aims to focus on diabetic patients and the possible oral complications that can occur in oral surgery sessions.
The MEDLINE database (NCBI PubMed and PMC) was employed to obtain sources of information. An online search was performed to select articles regarding complications in oral surgery for diabetic patients.
The search considered 52 articles using the following keywords: “diabetic patient,” “complications,” and “oral surgery.”
Although it is not possible to establish with certainty what the predisposing factors are, the scientific literature shows that the altered composition of saliva, together with the reduced immune response against bacteria, determines a lower antibacterial capacity in diabetic patients and, thus a higher frequency of inflammatory diseases caused by the accumulation of plaque and tartar; in particular, gingivitis, periodontitis, caries, aphthae or aphthous stomatitis, map tongue (benign migratory glossitis), xerostomia, tongue inflammation, and candidiasis.
In conclusion, the dentist must always take due account of all the needs of diabetic patients, trying to operate above all at the level of prevention and being aware of all the possible complications that diabetic patients could face during an oral surgery session.
Conscious sedation in dentistry: narrative review
Authors: Cusenza Irene , Pensa Vittorio , Rastelli Sofia , Galati Chiara , Cogotzi Stefano , D'Orto Bianca , Nagni Matteo 
Published: February 2025

Purpose. The purpose of this narrative review is to demonstrate that conscious sedation is an anesthesiologic technique that, when appropriately performed, proves to be safe and valuable for the treatment of many cases that require its application such as: dental phobia patients, special needs patients, or simply to increase patient compliance in the case of investigative or very lengthy procedures.
Methods. All articles dealing with the topic of management of conscious sedation in dentistry were searched on the online platforms of scientific reading sites such as Pubmed and Medline, selecting the most current ones up to January 2022, including all languages. Considering the keyword “Sedation,” “ Dentistry,” “ Special needs,” “Dentophobia,” randomized controlled trials, prospective studies, observational studies, reviews, and retrospective studies were considered. Textbooks relevant to the topic were then reviewed, and citations of each retrieved article and those of expert opinions were examined to include as much information as possible.
Results. From the analysis of data found in the literature, it is possible to state that sedation is a special form of anesthesia that reduces pain sensitivity and induces myorelaxation, without impairing the patient’s consciousness. Moreover, it, which can be obtained by inhalation or pharmacological means, allows the patient to maintain autonomous breathing and to respond to physical and verbal stimuli.
Conclusion. Sedation in dentistry allows many patients to cope with dental sessions, reducing anxiety and emotional stress by eliminating the pain component. With conscious sedation, it is possible to bring the patient into a more relaxed situation by reducing stress and increasing compliance.