bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemostatic properties of glucosamine‐based materials
Authors: Fischer Thomas H. , Bode Arthur P. , Demcheva Marina , Vournakis John N. 
Published: September 2006

AbstractGlucosamine‐ and N‐acetyl glucosamine‐containing polymers are being used in an increasing number of biomedical applications, including in products for surface (topical) hemostasis. The studies presented here investigate the relationship between the structure (conformation) and function (activation of hemostasis) of glucosamine‐based materials. Several polymer systems were studied, including fibers isolated from a microalgal source containing poly‐N‐acetyl glucosamine polymers that are organized in a parallel, hydrogen‐bonded tertiary structure and can be chemically modified to an antiparallel orientation; and gel formulation derivatives of the microalgal fibers consisting of partially deacetylated (F2 gel) and fully deacetylated (F3 gel) polymers. Comparison of the properties of the poly‐N‐acetyl glucosamine fiber‐derived materials with chitin, chitosan, and commercial chitosan‐based products are presented. Several studies were performed with the glucosamine‐based materials, including (1) an analysis of the ability of materials to activate platelets and turnover of the intrinsic coagulation cascade, (2) an examination of the viscoelastic properties of mixtures of platelet‐rich plasma and the glucosamine‐based materials via thromboelastography, and (3) scanning electron microscopic studies to examine the morphology of the glucosamine‐based materials. The results presented demonstrate that hemostatic responses to the glucosamine‐based materials studied are highly dependent on their chemical nature and tertiary/quaternary structure. The unique natural microalgal fibers were found to have strongly prohemostatic activity compared to the other materials studied. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Factors affecting the structure and properties of an injectable self‐setting calcium phosphate foam
Authors: Ginebra Maria‐Pau , Delgado José‐Angel , Harr Ingela , Almirall Amisel , Del Valle Sergio , Planell Josep A. 
Published: September 2006

AbstractOne of the main challenges in the investigation on calcium phosphate cements (CPC) lies in the introduction of macroporosity, without loosing the self‐setting ability and injectability, characteristic of the cement‐type materials. The benefits of macroporosity are related to the enhancement of bone regeneration mechanisms, such as angiogenesis and tissue ingrowth. In this work, the feasibility to obtain self‐setting injectable macroporous hydroxyapatite foams by the incorporation of a protein‐based foaming agent to a CPC is demonstrated. Albumen is combined with an α‐tricalcium phosphate [Ca3(PO4)2, α‐TCP] paste, which hydrolyzes to a calcium deficient hydroxyapatite during the setting reaction. A systematic study is presented, where the effect of different processing parameters is analyzed in terms of porosity, setting properties, injectability, and compressive strength. Self‐setting foams with porosities up to 70%, which maintain their porous structure after injection, are obtained. These injectable foams can be used both for direct in vivo applications and for the fabrication of low temperature tissue engineering scaffolds. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Histological assessment of titanium and polypropylene fiber mesh implantation with and without fibrin tissue glue
Authors: ten Hallers E.J. Olivier , Jansen John A. , Marres Henri A.M. , Rakhorst Gerhard , Verkerke Gijsbertus J. 
Published: September 2006

AbstractPolypropylene (PP) and titanium (Ti) meshes are well‐known surgical implants that provoke a relative low foreign body reaction. Firm stabilization of the implant is important to prevent migration and subsequent failure of the operation. Fibrin tissue glues are commercially available adhesives and are widely accepted and applied in the medical field for hemorrhage, surgical bleeding, support of wound healing, wound and tissue gluing, sealing, and closure but also as antiadhesive agent in certain applications. The objective of this study was to evaluate the additional histological effect of fibrin glue application combined with two different types of meshes. Six pieces of mesh of each were subcutaneously implanted for 3, 6, and 12 weeks, with and without fibrin glue. After excision, processing, and staining, light microscopic analysis was performed on the sections, using subjective histological description and histomorphometry. Capsule quality, capsule thickness, interstitial quality, and total score were evaluated. To compare the samples with glue and without glue, analysis of variance (ANOVA) tests were carried out. No complications were observed. In general, the glue remnants remained visible at 3 and 6 weeks of implantation, accompanied by an inflammatory reaction and macrophage activity. At 12 weeks, all samples showed good tissue integration without evidence of glue. Evidently, the samples with glue demonstrated a prolonged inflammatory response and were surrounded by fibrous tissue capsules that were significantly thicker compared with the samples without glue (p < 0.05). © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Relationship of solvent to the photopolymerization process, properties, and structure in model dentin adhesives
Authors: Ye Qiang , Spencer Paulette , Wang Yong , Misra Anil 
Published: September 2006

AbstractThe ratio of the double‐bond content of monomer to polymer, i.e. degree of conversion (DC) has been used frequently as a convenient means of comparing the behavior and properties of dental composites and adhesives. The purpose of this investigation was to study the relationship of photopolymerization processes, bulk properties, and structure using model dentin adhesives cured in the presence of different ethanol content as an example. There was little difference in the DC of model BisGMA‐based adhesives cured in the presence of ethanol concentrations ranging from 0 to 40 wt %, but there were substantial differences in the mechanical properties. Ultimate tensile strength (UTS) and modulus of elasticity decreased with an increase in ethanol content. Polymer structure was revealed by thermal behavior in the glass transition temperature (Tg) region; these measurements were obtained by modulated temperature differential scanning calorimetry (MTDSC) technology, which removes the competing irreversible effects associated with release of volatiles and residual curing. Glass transition temperature of model adhesives decreased substantially with an increase in ethanol content. The DC based on the quantity of remaining double bond has been used extensively to characterize and provide a relative assessment of the quality of dentin adhesives and dental composites. Since polymers differing in linearity, and therefore crosslink density, may have a similar degree of conversion, the measurement of monomer/polymer conversion does not necessarily provide complete representation of the quality or durability of the polymer structure. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
iNOS‐mediated generation of reactive oxygen and nitrogen species by biomaterial‐adherent neutrophils
Authors: Patel Jasmine D. , Krupka Tianyi , Anderson James M. 
Published: September 2006

AbstractInfection due to implanted cardiovascular biomaterials is a serious complication initiated by bacterial adhesion to the surface of the implant. The release of reactive oxygen species by neutrophils, particularly superoxide anion, is a well‐known bactericidal mechanism. Additionally, nitric oxide (NO) has also been identified as an important cytotoxic mediator in acute and chronic inflammatory responses with enhanced NO production by upregulation of inducible nitric oxide synthase (iNOS). The interaction of NO and superoxide anion will result in the formation of peroxynitrite (OONO−), a potent cytotoxic oxidant. In this study, we have shown that biomaterial‐induced neutrophil activation does not cause upregulation of iNOS and activation of iNOS‐mediated pathways. However, NO and O production does occur over time upon adhesion to a biomaterial and is modulated by biomaterial surface chemistry. With no stimulus, the polyethylene oxide‐modified polyurethane induced greater neutrophil activation than did the control as indicated by the increased production of NO and O over time. Adherent‐stimulated neutrophils generally produced lower amounts of NO over time in comparison with unstimulated cells. Furthermore, there is no evidence of peroxynitrite activity in unstimulated neutrophils adherent to the Elasthane 80A. However, upon stimulation with adherent Staphylococcus epidermidis, peroxynitrite formation did occur. Our results suggest that bactericidal mechanisms in neutrophils involving NO generation (NOS pathway) are further compromised than O producing pathways (NADPH oxidase) upon exposure to biomaterials, resulting in a diminished microbial killing capacity, which can increase the probability of device‐centered infections. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Synthesis and characterization of biocompatible Fe3O4 nanoparticles
Authors: Sun Jing , Zhou Shaobing , Hou Peng , Yang Yuan , Weng Jie , Li Xiaohong , Li Mingyuan 
Published: September 2006

AbstractIn this study, magnetite (Fe3O4) nanoparticles with a size range of 8–20 nm were prepared by the modified controlled chemical coprecipitation method from the solution of ferrous/ferric mixed salt‐solution in alkaline medium. In the process, two kinds of surfactant (sodium oleate and polyethylene glycol) were studied; then, sodium oleate was chosen as the apt surfactant to attain ultrafine, nearly spherical and well‐dispersed (water‐base) Fe3O4 nanoparticles, which had well magnetic properties. The size and size distribution of nanoparticles were determined by particle size analyzer. And the magnetite nanoparticles was characterized by X‐ray powder diffraction (XRD) analysis, transmission electron microscopy (TEM), electron diffraction (ED) photography, Fourier transform infrared spectrometer (FT‐IR), and vibrating‐sample magnetometer (VSM). Also the effect of many parameters on the Fe3O4 nanoparticles was studied, such as reaction temperature, pH of the solution, stirring rate and concentration of sodium oleate. And the 5‐dimethylthiazol‐2‐yl‐2,5‐ diphenyltetrazolium bromide (MTT) assay was performed to evaluate the biocompatibility of magnetite nanoparticles. The results showed that the Fe3O4 nanoparticles coated by sodium oleate had a better biocompatibility, better magnetic properties, easier washing, lower cost, and better dispersion than the magnetite nanoparticles coated by PEG. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Amelogenin gene splice products A+4 and A−4 implanted in soft tissue determine the reorientation of CD45‐positive cells to an osteo‐chondrogenic lineage
Authors: Lacerda‐Pinheiro S. , Septier D. , Tompkins K. , Veis A. , Goldberg M. , Chardin H. 
Published: September 2006

AbstractSeveral molecules such as bone morphogenetic protein‐7, bone sialoprotein (BSP), or amelogenin gene splice products (A+4 or A−4) have been shown to induce reparative dentin formation in a rat model. However, at the moment, the origin and the mechanism of differentiation of the pulp cells stimulated by the bioactive molecules remain poorly understood. The present investigation was undertaken to validate an ectopic oral mucosal mouse model to evaluate the effects of amelogenin gene splice product implantation in a non‐mineralizing tissue. Agarose beads, alone or coated with amelogenin gene splice products, were implanted in the mucosa of the cheeks in mouse. An immunohistochemical characterization of the recruited cells was undertaken for 3 days, 8 days, and 30 days after the implantation. The results showed that the implantation of agarose beads in mucosa induced the recruitment of inflammatory CD45 positive cells. When the beads were coated with amelogenin gene splice products (A+4 or A−4), the expression of osteo‐chondrogenic markers (RP59, Sox9, or BSP) was also observed. However, no mineralization nodule was observed, even after 30 days of implantation. The present investigation suggests that amelognin gene splice products have the capacity of recruiting among inflammatory cell mesenchymal progenitors that eventually differentiate into osteo‐chondrogenic cells. Altogether, the results obtained in the pulp model and the present data suggest the existence of different pathways of cell recruitment and differentiation in different cellular environments. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Tumor progression—targets for differential therapy
Authors: Pardee Arthur B. 
Published: September 2006

AbstractDifferential killing of the patient's cancer cells versus normal cells is a necessity for chemotherapy. Advantage can be taken of close regulations of gene expression and of enzyme activity that are essential for normal cell functioning, and that are altered during tumor progression. Summarized here is our research on four such progression changes of cancer cells; some deregulate proliferation control and others decrease programmed death (apoptosis). These processes will be illustrated with examples of potential chemotherapies based on them. Methods for discovery of such changes include Differential Display and microarrays. J. Cell. Physiol. 209: 589–591, 2006. © 2006 Wiley‐Liss, Inc.
Cell senescence: Hypertrophic arrest beyond the restriction point
Authors: Blagosklonny Mikhail V. 
Published: September 2006

AbstractWithdrawal of mitogens (growth factors) arrests normal cells in G0 (quiescence). All other stresses and factors arrest cell cycle beyond the restriction point in G1 and G2 (non‐G0 arrest), in the presence of mitogenic stimulation. Strong mitogenic stimuli by themselves cause non‐G0 arrest. Unlike G0, arrest beyond restriction point is characterized by both high levels of cyclins and CDK inhibitors, activated mitogenic pathways with a secondary GF resistance, and continuous mass growth (cell hypertrophy). Prolonged hypertrophic arrest culminates in cell senescence. This review discusses that quiescence and senescence are two opposite, mutually exclusive conditions and that cell senescence can be reversed and prevented. J. Cell. Physiol. 209: 592–597, 2006. © 2006 Wiley‐Liss, Inc.
Biological functions of maspin
Authors: Bailey Caleb M. , Khalkhali‐Ellis Zhila , Seftor Elisabeth A. , Hendrix Mary J.C. 
Published: September 2006

AbstractMaspin (Mammary Serine Protease Inhibitor) was first reported in 1994 as a serpin with tumor suppressive properties. Maspin was initially isolated through subtractive hybridization and differential display analysis as a 42‐kDa protein that is expressed in normal mammary epithelial cells but reduced or absent in breast carcinomas (Zou et al., 1994). Further research led to maspin's characterization as a class II tumor suppressor based on its ability to inhibit cell invasion, promote apoptosis, and inhibit angiogenesis (Sheng et al., 1996; Zhang et al., 2000b; Jiang et al., 2002). Since then, efforts have been made to characterize maspin's tumor suppressive mechanisms. In particular, researchers have studied maspin localization, the regulation of maspin expression, and more recently, maspin protein interactions. By elucidating these mechanisms, researchers are beginning to understand the complex, pleiotropic nature of maspin and the pathways through which maspin exerts its tumor suppressive properties. These new findings not only further enhance our understanding of cancer biology but also provide an avenue to develop maspin's potential as a diagnostic marker for cancer progression, and as a potentially powerful therapeutic agent in the fight against breast cancer. J. Cell. Physiol. 209: 617–624, 2006. © 2006 Wiley‐Liss, Inc.
Linkage between EGFR family receptors and nuclear factor kappaB (NF‐κB) signaling in breast cancer
Authors: Biswas Debajit K. , Iglehart J. Dirk 
Published: September 2006

AbstractIn the United States there are 225,000 new cases of invasive breast cancer annually, and at least 50,000 women are diagnosed with ductal carcinoma in situ (DCIS). Breast cancer is a collection of disorders of mammary epithelial cells with distinct pathological characteristics and diverse clinical manifestations. Breast cancers are divided broadly into four classes by the level of the biomarkers HER2 (erbB2/neu) and the estrogen receptor (ER). Histologic grade is also an important modifier of breast cancer taxonomy and behavior. Broadly speaking, breast cancer can be divided into those that are HER2‐positive, containing cancers that are both ER‐positive and negative, cancers that are ER‐positive and divided into high‐grade and low‐grade tumors, and the remaining but important class of cancers that are both ER‐negative and HER2‐negative. These last cancers are called basal‐like and were first recognized as a distinct group by gene expression arrays. Nuclear factor kappaB (NF‐κB) is family of multifunctional transcription factors that when activated generate pleotrophic changes in target cells. Elevated levels of active NF‐κB are detected in many human diseases including breast cancers. High‐level active NF‐κB is detected in specific subclasses of breast cancers briefly described above, predominantly in ER‐negative and epidermal growth factor family receptor (EGFR) overexpressing breast cancers (predominantly HER2 amplified cancers). This article is focused on the role of NF‐κB activation initiated by the EGFR family receptors in subclasses of breast cancer. The combined influence of EGFR family receptors and NF‐κB signaling on the transformation of ER‐negative human mammary epithelial cell is illustrated. J. Cell. Physiol. 209: 645–652, 2006. © 2006 Wiley‐Liss, Inc.
Transcriptome profiling in clinical breast cancer: From 3D culture models to prognostic signatures
Authors: Fournier Marcia V. , Martin Katherine J. 
Published: September 2006

AbstractEarly detection has been one of the most effective strategies to control the growing cancer burden. The power of earlier detection has been demonstrated by the impact of pap‐smear, mammography, and PSA tests on cancer patient treatment and survival. These tests benefit patients independent of their genetic background or race. However, in many cases, we are still losing the battle against cancer because patients that initially presented with low‐grade disease progress rapidly to aggressive forms of the disease. As of yet, we have limited means to predict a particular patient's fate or to specifically treat subtypes of cancer. A combination of earlier detection and targeted therapy, based on information from transcriptome analysis, could be a powerful ally in this battle. The theme of this review article is to briefly summarize innovative strategies using three‐dimensional (3D) cell cultures of human mammary epithelial cells to predict clinical outcome in breast cancer. This strategy has the potential to further enhance our understanding of breast cancer biology and to contribute to the identification of biologically significant bio‐markers that are also useful drug targets. J. Cell. Physiol. 209: 625–630, 2006. © 2006 Wiley‐Liss, Inc.
From differential display to DNA microarrays—a personal account
Authors: Liang Peng 
Published: September 2006

AbstractThis article is a tribute to Dr. Arthur Pardee, one of the most innovative and brilliant scientists of our time, on the occasion of his 85th birthday. In this partially perspective and partially review piece, I look back how fate, by twist and turn, has led me eventually to his lab at Harvard where we worked out the Differential Display technology from scratch, how the method has revolutionized the field of gene expression analysis and where DD is taking us in the “era” of DNA microarrays. J. Cell. Physiol. 209: 653–658, 2006. © 2006 Wiley‐Liss, Inc.
Discovering innate immunity genes using differential display: A story of RNA helicases
Authors: Ramirez‐Ortiz Zaida G. , Warke Rajas V. , Pacheco Laura , Xhaja Kris , Sarkar Devanand , Fisher Paul B. , Shaw Sunil K. , Martin Katherine J. , Bosch Irene 
Published: September 2006

AbstractIn this review we discuss the current literature for RNA helicases in response to RNA virus infection. We show the use of Differential Display Reverse Transcription PCR methodology (DD) to analyze virus–host interactions and we present current findings in dengue virus‐induced gene expression of RNA helicases. J. Cell. Physiol. 209: 636–644, 2006. © 2006 Wiley‐Liss, Inc.
Lessons learned from art pardee in cell cycle, science, and life
Authors: Dou Q. Ping 
Published: September 2006

AbstractThis essay is written to honor Dr Art Pardee's 85th birthday (July 13, 2006). In this essay, I have summarized the lessons I learned from Art and the cell‐cycle research I performed in Art's laboratory during my postdoctoral training period. I have also summarized some research from my own laboratory that has been inspired by the lessons I learned from Art, including the interactions between cell cycle and cell death regulators and discovery of novel polyphenol‐ and copper‐based proteasome inhibitors. Finally, I have discussed the potential use of these proteasome inhibitors in cancer prevention and treatment. J. Cell. Physiol. 209: 663–669, 2006. © 2006 Wiley‐Liss, Inc.
Therapeutic biology: Checkpoint pathway activation therapy, HIV Tat, and transkingdom RNA interference
Authors: Li Chiang J. 
Published: September 2006

AbstractTherapeutic biology is an exciting new frontier in the post‐genomic era with the mission to better human health. The explosive increase in the understanding of molecular and regulatory biology has enabled the identification of a plethora of potential targets and pathways for the discovery of new medicines. Conversely, molecularly based therapeutic intervention of biological aberrations in human diseases offers a test of the depth of our understanding of biology. This article discusses three examples of therapeutic biology research. The first concerns the treatment of cancer by activating genome surveillance circuitry, namely checkpoint‐pathway activation therapy. The second example is the identification of the HIV Tat protein as both a cause of immune cell activation and apoptosis, and as a vaccine candidate against HIV infection. The third example describes transkingdom RNA interference and its application in the investigational and therapeutic silencing of disease genes. J. Cell. Physiol. 209: 695–700, 2006. © 2006 Wiley‐Liss, Inc.