bluuebunny/crossref_metadata_2025_split huggingface dataset:
The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells
Authors: Murayama Yoko , Shinomura Yasuhisa , Oritani Kenji , Miyagawa Jun‐Ichiro , Yoshida Hitoshi , Nishida Makoto , Katsube Fumie , Shiraga Masamichi , Miyazaki Tamana , Nakamoto Taisei , Tsutsui Shusaku , Tamura Shinji , Higashiyama Shigeki , Shimomura Iichirou , Hayashi Norio 
Published: February 2008

AbstractCD9 is a member of the tetraspanins, and has been shown to be involved in a variety of cellular activities such as migration, proliferation, and adhesion. In addition, it has been known that CD9 can associate with other proteins. Here we demonstrated the physical and functional association of CD9 with epidermal growth factor receptor (EGFR) on MKN‐28 cells. Double‐immunofluorescent staining and immunoprecipitation demonstrated the complex formation of CD9‐EGFR and CD9‐β1integrin, and that both complexes are colocalized on the cell surface especially at the cell–cell contact site. Anti‐CD9 monoclonal antibody ALB6 induced a dotted or patch‐like aggregation pattern of both CD9‐EGFR and CD9‐β1integrin. The internalization of EGFR after EGF‐stimulation was significantly enhanced by the treatment with ALB6. CD9 can associate with EGFR in hepatocellular carcinoma cells (HepG2/CD9) and Chinese hamster ovary cancer cells (CHO‐HER/CD9), which were transfected with pTJ/human EGFR/CD9. Furthermore expression of CD9 specifically attenuated EGFR signaling in CHO‐HER/CD9 cells through the down regulation of surface expression of EGFR. These results suggest that CD9 might have an important role that attenuates EGFR signaling. Therefore, CD9 not only associates EGFR but also a new regulator, which may affect EGF‐induced signaling in cancer cells. J. Cell. Physiol. 216: 135–143, 2008. © 2008 Wiley‐Liss, Inc.
Overexpression of bax induces down‐regulation of store‐operated calcium entry in prostate cancer cells
Authors: Li Na , Zheng Lin , Lin Peihui , Danielpour David , Pan Zui , Ma Jianjie 
Published: February 2008

AbstractStore‐operated Ca2+ channels control homeostasis between extracellular Ca2+ reservoir and intracellular Ca2+ storage and play important roles in apoptosis in a wide variety of cells, including prostate epithelia. Recent studies have shown that the acquired apoptosis‐resistant nature of androgen‐independent prostate cancer is associated with reduced function of store‐operated Ca2+ entry (SOCE). This study investigates the functional interaction between Bax and SOCE in the apoptosis signaling cascade in prostate cancer. Our previous findings show that NRP‐154, an androgen‐independent prostate cancer cell line, could sustain overexpression of exogenous Bax without undergoing apoptosis. Here we show that sustained overexpression of Bax in NRP‐154 cells leads to down‐regulation of SOCE and reduced Ca2+ storage inside the endoplasmic reticulum. While reduced SOCE may represent an adaptive mechanism for cell survival, increased levels of Bax in the latent state enhances the sensitivity of NRP‐154 cells to TGF‐β and thapsigargin‐induced apoptosis. This enhanced apoptosis can be reduced by 2‐aminoethoxydiphenyl borate (2‐APB), an inhibitor of SOCE, or reversed under conditions where SOCE is only partially activated. Our results demonstrate a functional interaction between Bax and SOCE in apoptosis of prostate cancer, and support the concept that improving this interaction has therapeutic implications for prostate cancer. J. Cell. Physiol. 216: 172–179, 2008. © 2008 Wiley‐Liss, Inc.
TRPC5 channels undergo changes in gating properties during the activation‐deactivation cycle
Authors: Obukhov Alexander G. , Nowycky Martha C. 
Published: February 2008

AbstractTRPC5 are non‐specific cation channels activated through phospholipase C‐dependent pathways, although the precise gating mechanism is unknown. TRPC5 current–voltage relationships (I–Vs) change systematically during the activation‐deactivation cycle, shifting between outwardly rectifying and doubly rectifying shapes. Since several TRP family members exhibit voltage‐dependent properties, we investigated whether the various I–V relationships were due to changes in gating. Using patch‐clamp recordings of rat TRPC5 transfected HEK293 cells, we found that TRPC5 currents had distinct biophysical characteristics correlated with individual I–V shapes, a phenomenon we call ‘phases.’ At rest, channels were closed at most potentials, although strong depolarizations (>+80 mV) stimulated small outward currents (Phase 0). For 10–15 sec after activation, voltage steps evoked small inward and large outward currents with time‐ and voltage‐dependent kinetics (Phase 1, outwardly‐rectifying I–Vs). At maximal inward amplitude, currents were voltage‐independent at all potentials (Phase 2, doubly‐rectifying I–Vs owing to Mg2+ block). During desensitization (Phase 3), currents reverted to a Phase 1‐like voltage‐dependence. La3+ ions potentiated inward TRPC5 currents by promoting a reversible transition from Phase 3 to Phase 2. Single channel recordings revealed asymmetric conductance properties with values of ∼40 pS at negative potentials and ∼130 pS at >+60 mV. Mutation of D633, a cytoplasmic residue that mediates Mg2+ block, decreased channel activity at negative potentials during Phase 2. We conclude that TRPC5 gating properties can switch reversibly between voltage‐dependent and voltage‐independent states. The modulation of phase transitions by external agents such as La3+ and EBP50, a scaffolding protein, may constitute a novel mechanism for regulation of channel activity. J. Cell. Physiol. 216: 162–171, 2008. © 2008 Wiley‐Liss, Inc.
Elk‐1 associates with FAK, regulates the expression of FAK and MAP kinases as well as apoptosis in HK‐2 cells
Authors: Mamali Irene , Kotsantis Panagiotis , Lampropoulou Maria , Marmaras Vassilis J. 
Published: February 2008

AbstractFocal adhesion kinase (FAK), MAP kinases and the nuclear transcription factor Elk‐1 have been reported to be implicated in the same cellular processes, however, their direct or indirect interaction and potential function(s) has not been documented. Here, we explored the association of FAK with Elk‐1, the implication of Elk‐1 in the regulation of FAK and MAP kinases expression as well as apoptosis, in HK‐2 cells. Biochemical and immunofluorescence approaches strongly support the association of low molecular weight protein bands, recognized by FAK antibodies, with Elk‐1 or pser383Elk‐1. The FAK/Elk‐1 complex is found, mainly, in the cytoplasm, near the nuclear membrane periphery, raising the possibility that Elk‐1 may have alternative extranuclear function(s) in HK‐2 cells. Furthermore, we demonstrated that Elk‐1 siRNA‐mediated knockdown experiments, increased apoptosis. By contrast, Elk‐1 siRNA decreased significantly the expression of FAK and MAP kinases, supporting the hypothesis that Elk‐1 may act as a potential physiological substrate and regulator of FAK and MAP kinases expression. These results strongly support that Elk‐1 protein is a novel binding‐protein partner for FAK, a finding that significantly broadens the potential functioning of FAK and Elk‐1. J. Cell. Physiol. 216: 198–206, 2008. © 2008 Wiley‐Liss, Inc.
Update from the 7th annual meeting of the Italian society of virology
Authors: Salata Cristiano , Calistri Arianna , Palù Giorgio 
Published: February 2008

AbstractThe Italian Society of Virology (SIV) held a meeting in Orvieto (June 24–26, 2007) aimed at promoting interactions and collaborations between scientists in the field of Virology. The meeting had an attendance of about 170 virologists from Italy. In accordance with the normal format of the SIV National Meeting the conference transcended all areas of Virology. Sessions included invited speakers together with selected oral presentation. Covered topics included: General Virology and Viral Genetics, Medical Virology and Antiviral Therapy, Viral Biotechnologies and Gene Therapy, Viral Oncogenesis and Vaccines, Virus–Host Interactions and Pathogenesis, Emerging and Zoonotic Viral Infections. In this edition, a special effort was addressed to the HPV infection and prevention and to the guidelines for the preemptive (presymptomatic) therapy of human cytomegalovirus infections in transplant recipients. A summary of the main topics are reported. J. Cell. Physiol. 216: 286–288, 2008. © 2008 Wiley‐Liss, Inc.
Laminar shear stress stimulates vascular smooth muscle cell apoptosis via the Akt pathway
Authors: Fitzgerald Tamara N. , Shepherd Benjamin R. , Asada Hidenori , Teso Desarom , Muto Akihito , Fancher Tiffany , Pimiento Jose M. , Maloney Stephen P. , Dardik Alan 
Published: February 2008

AbstractVascular smooth muscle cells (SMC) may be directly exposed to blood flow after an endothelial‐denuding injury. It is not known whether direct exposure of SMC to shear stress reduces SMC turnover and contributes to the low rate of restenosis after most vascular interventions. This study examines if laminar shear stress inhibits SMC proliferation or stimulates apoptosis. Bovine aortic SMC were exposed to arterial magnitudes of laminar shear stress (11 dynes/cm2) for up to 24 h and compared to control SMC (0 dynes/cm2). SMC density was assessed by cell counting, DNA synthesis by 3[H]‐thymidine incorporation, and apoptosis by TUNEL staining. Akt, caspase, bax, and bcl‐2 phosphorylation were assessed by Western blotting; caspase activity was also measured with an in vitro assay. Analysis of variance was used to compare groups. SMC exposed to laminar shear stress had a 38% decrease in cell number (n = 4, P = 0.03), 54% reduction in 3[H]‐thymidine incorporation (n = 3, P = 0.003), and 15‐fold increase in TUNEL staining (n = 4, P < 0.0001). Akt phosphorylation was reduced by 67% (n = 3, P < 0.0001), whereas bax/bcl‐2 phosphorylation was increased by 1.8‐fold (n = 3, P = 0.01). Caspase‐3 activity was increased threefold (n = 5, P = 0.03). Pretreatment of cells with ZVAD‐fmk or wortmannin resulted in 42% increased cell retention (n = 3, P < 0.01) and a fourfold increase in apoptosis (n = 3, P < 0.04), respectively. Cells transduced with constitutively‐active Akt had twofold decreased apoptosis (n = 3, P < 0.002). SMC exposed to laminar shear stress have decreased proliferation and increased apoptosis, mediated by the Akt pathway. These results suggest that augmentation of SMC apoptosis may be an alternative strategy to inhibit restenosis after vascular injury. J. Cell. Physiol. 216: 389–395, 2008. © 2008 Wiley‐Liss, Inc.
Hepatic endopolyploidy as a cellular consequence of age‐specific selection for rate of development in mice
Authors: Funk‐Keenan Jhondra , Haire Frances , Woolard Sara , Atchley William R 
Published: February 2008

AbstractEndopolyploidy is the generation of polyploid cells by DNA replication without subsequent cell division and is correlated with hypertrophic growth or growth via cell size. Thus, selection that alters growth may also change onset and frequency of endopolyploidy as a correlated response. We search for endopolyploidy in the liver in response to age‐specific restricted index selection for the rate of development. Polyploidy changes over ontogeny are described in five mouse lines: two selected for divergence in early growth (0–10 days of age), two selected for divergence in late growth (28–56 days of age), and one randombred control. Polyploid cell frequency within each line increased as ontogeny continued, as expected from previous research. However, selection for altered growth clearly plays a role in the frequency and onset of polyploid cells. Lines selected for divergence in early growth have polyploidy differences after weaning that are not seen in adult mice. However, lines selected for divergence in late growth are divergent in frequency of polyploid cells, starting near sexual maturity and continuing into adulthood. J. Exp. Zool. (Mol. Dev. Evol.) 310B:385–397, 2008. © 2008 Wiley‐Liss, Inc.
Probing the interaction between p53 and the bacterial protein azurin by single molecule force spectroscopy
Authors: Taranta Monia , Bizzarri Anna Rita , Cannistraro Salvatore 
Published: February 2008

Abstractp53 is a human tumour suppressor which regulates multiple cellular processes, including cell growth, genomic stability and cell death. Recent works have demonstrated the bacterial redox protein azurin to enter cancer cells and induce apoptosis through p53 stabilization, resulting in a tumour growth regression. Azurin has been shown to bind p53 although many details of the complex formed by these two proteins are still poorly characterized. Here, we get insight into the kinetics of this complex formation, by exploring the interaction between p53 and azurin in their environment by single molecule force spectroscopy. To this aim, azurin has been linked to the atomic force microscope tip, whereas p53 has been immobilized onto a gold substrate. Therefore, by performing force‐distance cycles we have detected specific recognition events between p53 and azurin, displaying unbinding forces of around 70 pN for an applied loading rate of 3 nN s−1. The specificity of these events has been assessed by the significant reduction of their frequency observed after blocking the p53 sample by an azurin solution. Moreover, by measuring the rupture force as a function of the loading rate we have determined the dissociation rate constant of this complex to be ∼0.1 s−1. Our findings are here discussed in connection with results obtained in bulk experiments, with the aim of clarifying some molecular details of the p53‐azurin complex that may help designing new anticancer strategy. Copyright © 2008 John Wiley & Sons, Ltd.
Protein‐responsive imprinted polymers with specific shrinking and rebinding
Authors: Chen Zhiyong , Hua Zhendong , Xu Li , Huang Yong , Zhao Meiping , Li Yuanzong 
Published: February 2008

AbstractStimuli‐responsive protein imprinted polymers were obtained via a combination of molecular imprinting and reversible stimuli‐responsive polymer using lysozyme or cytochrome c as template, N‐isopropylacrylamide (NIPA) as major monomer, methacrylic acid (MAA) and acrylamide (AAm) as functional co‐monomers, and N,N‐methylenebisacrylamide (MBAAm) as crosslinker. The molecularly imprinted polymers (MIPs) can respond not only to external stimuli such as temperature and salt concentration, but also to the corresponding template protein with significant specific volume shrinking. This specific shrinking behavior was attributed to the synergistic effect of multiple‐site weak interactions (electrostatic force, hydrogen bonding and hydrophobic interaction) and the cavity effect. The MIPs showed highly selective adsorption of template proteins with specific shrinking compared with the non‐imprinted polymers. The results indicated that the MIPs seemed to change shape to accommodate the conformation of the template protein leading to the formation of a shape complementary cavity. Copyright © 2008 John Wiley & Sons, Ltd.
Gentamicin production by Micromonospora echinospora (Me‐ 22) in stirred tank reactor: effect of various parameters
Authors: Meenavilli Himabindu , Potumarthi Ravichandra , Jetty Annapurna 
Published: February 2008

AbstractEffect of production medium components, initial starch and soyabean meal concentrations, for the enhanced production of gentamicin by Micromonospora echinospora (Me‐ 22) was studied in a lab scale stirred tank reactor. Also effect of different aeration (0.5, 1, 2, and 4 vvm) and agitation rates (100, 200, 300 and 400 rpm) in a stirred tank reactor was examined. A maximum gentamicin concentration of 2.68 g l–1 was achieved in the medium having low concentrations of initial starch (7.5 g l–1) and high concentrations of initial soyabean meal (4 g l–1). Both aeration and agitation significantly affected gentamicin concentration, productivity and biomass formation. The maximum gentamicin concentration of 4.12 g l–1 and the highest yield of gentamicin on substrate 0.967 g g–1 were obtained at impeller speed of 200 rpm and aeration rate of 2 vvm. Under optimal culture conditions in STR the production of gentamicin could be increased 3 fold when compared with shake flask. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Typing of Staphylococcus epidermidis clinical strains by a selected panel of Brazilian killer yeasts
Authors: Fuentefria Alexandre M. , Perez Leandro R. R. , Alves d'Azevedo Pedro , Pazzini Fabiano , Schrank Augusto , Vainstein Marilene H. , Valente Patricia 
Published: February 2008

AbstractDiscrimination of multi‐resistant microorganisms in small clinical microbiology laboratories is frequently based on the biologic profile (biotype) of phenotypic markers, such as antimicrobial susceptibility patterns (antibiograms) and serologic or enzymatic typing, but few use indicative microorganisms. The purpose of this study was to evaluate the power of a panel of selected killer yeasts for differentiating and discriminating clinical isolates of Staphylococcus epidermidis from two care hospitals and clinical microbiology laboratories from the South of Brazil. The short panel of eleven killer yeasts was capable of discriminating 100% of the sensitive strains of S. epidermidis using quantitative data matrix (QDM) and differentiating them from strains of coagulase‐positive Staphylococcus. Therefore, this phenotypic methodology proved to be valid as a discriminatory tool when applied to these clinical bacteria strains, besides being simple and feasible for routine use even in microbiological laboratories with minimal resources. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Isolation and molecular characterization of Shewanella sp. G5, a producer of cold‐active β ‐D‐glucosidases
Authors: Cristóbal Héctor Antonio , Breccia Javier Darío , Abate Carlos Mauricio 
Published: February 2008

Abstractβ ‐Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 °C using different β ‐glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high β ‐glucosidase activity on plate at low temperature (4 and 20 °C). Two genes encoding different cold‐active β ‐glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W
Authors: Sahu Bhubanananda , Ray Malay K. 
Published: February 2008

AbstractA chemically defined minimal medium has been developed for growing the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W, a model system for studying cold adaptation. This natural isolate from Antarctica has an absolute requirement for two branched chain amino acids, isoleucine and valine, in addition to low osmolality of the growth medium. The bacterium contains threonine deaminase but lacks acetohydroxyacid synthase suggesting that a defect lies in the isoleucine and valine biosynthetic pathway causing auxotrophy. Succinate was found to be preferred carbon source over glucose as it could suppress the glucose metabolizing enzymes in the cells, like in other pseudomonads. The development of the minimal medium (MMLz) for growing the Antarctic P. syringae Lz4W strain would be useful for investigation of the catabolite repression control mechanism at a very low temperature (below 5 °C), which is predominant in vast area of our global ecosystems. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Agrobacterium tumefaciens ‐mediated transformation of the zygomycete fungus Backusella lamprospora
Authors: Nyilasi Ildikó , Papp Tamás , Csernetics Árpád , Vágvölgyi Csaba 
Published: February 2008

AbstractThe Agrobacterium ‐mediated transformation was adapted to Backusella lamprospora, a zygomycete fungus closely related to Mucor. The transforming plasmid contained the hygromycin B resistance (hph) and the green fluorescent protein (gfp) genes under the control of the regulator regions of the Mucor circinelloides gpd1 gene. The presence of the hph and gfp genes in the transformants was detected by PCR. The introduced genes could also be amplified directly from the spores of the transformants. The transformation efficiency was investigated by fluorescence microscopy of the transformed spores. A gradual decrease in the hygromycin B resistance was observed during several cultivation cycles: the growth of the transformants on the selection medium became slower, and the detection of the introduced gene became more difficult. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Microbial diversity of culturable heterotrophs in the rhizosphere of salt marsh grass, Porteresia coarctata (Tateoka) in a mangrove ecosystem
Authors: Bharathkumar Srinivasan , Paul Diby , Nair Sudha 
Published: February 2008

AbstractA study was conducted to understand the complexity of bacterial diversity of rhizosphere of Porteresia coarctata based on culture dependent method. A large number of bacteria were isolated on nutrient agar medium supplemented with 1% NaCl and the dominant ones were further analyzed with PCR‐RFLP method. The sequence analyses of the dominant strains revealed that most of the sequences belonged to members of gamma proteobacteria, firmicutes, bacteroidetes and uncultured bacteria. The phylogenetic analysis of 16S rRNA gene sequences revealed close relationships to a wide range of clones or bacterial species of various divisions. These results afford an understanding of the role of rhizobacteria in alleviating salt stress in Porteresia coarctata expected to contribute towards long‐term goal of improving plant‐microbe interactions for salinity affected fields. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Curdlan production by Agrobacterium sp. ATCC 31749 on an ethanol fermentation coproduct
Authors: West Thomas P. , Nemmers Beth 
Published: February 2008

AbstractThe production of the polysaccharide curdlan from the ethanol processing coproduct condensed corn distillers solubles by the bacterium Agrobacterium sp. ATCC 31749 was investigated. It was found that curdlan could be produced by the bacterium using condensed corn distillers solubles as a source of carbon and nitrogen. As the concentration of condensed corn distillers solubles was increased from 50 g l–1 to 400 g l–1, the concentration of curdlan increased but not proportionally. The highest curdlan concentration was produced by the strain on 400 g l–1 condensed corn distillers solubles after 120 h and its level was higher than was observed for glucose‐based curdlan production. Biomass production by ATCC 31749 was also highest after 120 h of growth on 400 g l–1 condensed corn distillers solubles and was higher than found for glucose‐based biomass production. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)