bluuebunny/crossref_metadata_2025_split huggingface dataset:
Partial Functional Complementation of a Pseudorabies Virus UL25 Deletion Mutant by Herpes Simplex Virus Type 1 pUL25 Indicates Overlapping Functions of Alphaherpesvirus pUL25 Proteins
Authors: Kuhn Jana , Leege Tobias , Klupp Barbara G. , Granzow Harald , Fuchs Walter , Mettenleiter Thomas C. 
Published: April 2008

ABSTRACT

Homologs of the UL25 gene product of herpes simplex virus 1 (HSV-1) are highly conserved among the
Herpesviridae
. However, their exact function during viral replication is unknown. Current evidence suggests that in the alphaherpesvirus pseudorabies virus (PrV) the capsid-associated pUL25 plays a role in primary envelopment of DNA-containing mature capsids at the inner nuclear membrane. In the absence of pUL25, capsids were found in close association with the inner nuclear membrane, but nuclear egress was not observed (B. G. Klupp, H. Granzow, G. M. Keil, and T. C. Mettenleiter, J. Virol. 80:6235-6246, 2006). In contrast, HSV-1 pUL25 has been assigned a role in stable packaging of viral genomes (N. Stow, J. Virol. 75:10755-10765, 2001). Despite these apparently divergent functions, we wanted to assess whether the high sequence homology translates into functional homology. Therefore, we first analyzed a newly constructed HSV-1 UL25 deletion mutant in our assay system and observed a similar phenotype as in PrV. In the nuclei of infected cells, numerous electron-dense C capsids were detected, whereas primary envelopment of these capsids did not ensue. In agreement with results from PrV, vesicles were observed in the perinuclear space. Since these data indicated functional homology, we analyzed the ability of pUL25 of HSV-1 to complement a PrV UL25 deletion mutant and vice versa. Whereas a HSV-1 pUL25-expressing cell line partially complemented the pUL25 defect in PrV, reciprocal complementation of a HSV-1 UL25 deletion mutant by PrV pUL25 was not observed. Thus, our data demonstrate overlapping, although not identical functions of these two conserved herpesvirus proteins, and point to a conserved functional role in herpes virion formation.
During Herpes Simplex Virus Type 1 Infection of Rabbits, the Ability To Express the Latency-Associated Transcript Increases Latent-Phase Transcription of Lytic Genes
Authors: Giordani Nicole V. , Neumann Donna M. , Kwiatkowski Dacia L. , Bhattacharjee Partha S. , McAnany Peterjon K. , Hill James M. , Bloom David C. 
Published: April 2008

ABSTRACT

Trigeminal ganglia (TG) from rabbits latently infected with either wild-type herpes simplex virus type 1 (HSV-1) or the latency-associated transcript (LAT) promoter deletion mutant 17ΔPst were assessed for their viral chromatin profile and transcript abundance. The wild-type 17
syn
+ genomes were more enriched in the transcriptionally permissive mark dimethyl H3 K4 than were the 17ΔPst genomes at the 5′ exon and ICP0 and ICP27 promoters. Reverse transcription-PCR analysis revealed significantly more ICP4,
tk
, and glycoprotein C lytic transcripts in 17
syn
+ than in 17ΔPst. These results suggest that, for efficient reactivation from latency in rabbits, the LAT is important for increased transcription of lytic genes during latency.
Scaffolding Proteins Altered in the Ability To Perform a Conformational Switch Confer Dominant Lethal Assembly Defects
Authors: Cherwa James E. , Uchiyama Asako , Fane Bentley A. 
Published: April 2008

ABSTRACT

In the φX174 procapsid crystal structure, 240 external scaffolding protein D subunits form 60 pairs of asymmetric dimers, D
1
D
2
and D
3
D
4
, in a non-quasi-equivalent structure. To achieve this arrangement, α-helix 3 assumes two different conformations: (i) kinked 30° at glycine residue 61 in subunits D
1
and D
3
and (ii) straight in subunits D
2
and D
4
. Substitutions for G61 may inhibit viral assembly by preventing the protein from achieving its fully kinked conformation while still allowing it to interact with other scaffolding and structural proteins. Mutations designed to inhibit conformational switching in α-helix 3 were introduced into a cloned gene, and expression was demonstrated to inhibit wild-type morphogenesis. The severity of inhibition appears to be related to the size of the substituted amino acid. For infections in which only the mutant protein is present, morphogenesis does not proceed past the first step that requires the wild-type external scaffolding protein. Thus, mutant subunits alone appear to have little or no morphogenetic function. In contrast, assembly in the presence of wild-type and mutant subunits is blocked prematurely, before D protein is required in a wild-type infection, or channeled into an off-pathway reaction. These data suggest that the wild-type protein transports the inhibitory protein to the pathway. Viruses resistant to the lethal dominant proteins were isolated, and mutations were mapped to the coat and internal scaffolding proteins. The affected amino acids cluster in the atomic structure and may act to exclude mutant subunits from occupying particular positions atop pentamers of the viral coat protein.
International conference on physical activity and obesity in children: summary statement and recommendationsThis summary statement and recommendations from the International Conference on Physical Activity and Obesity in Children is being published simultaneously in the International Journal of Pediatric Obesity and Applied Physiology, Nutrition, and Metabolism.For the International Association for the Study of Obesity Physical Activity Task Force and the Conference Speaker Panel (the conference speaker panel includes Tom Baranowski, Claude Bouchard, Kelly Brownell, Deborah Cohen, William H. Dietz, Rod Dishman, Mary Flynn, William Haskell, James O. Hill, W.P.T. (Philip) James, Russell Pate, John Peters, Michael Pratt, Harry Rutter, James Sallis, Jo Salmon, Chantal Simon, and Boyd Swinburn).
Authors: Katzmarzyk Peter T. , Baur Louise A. , Blair Steven N. , Lambert Estelle V. , Oppert Jean-Michel , Riddoch Chris 
Published: April 2008

The increasing prevalence of obesity among the world’s children and youth was the impetus for an international conference convened in Toronto, Canada, to examine issues related to physical activity and obesity in children (24–27 June 2007). The goal of the conference was to assimilate, interpret, and share scientific evidence with key stakeholders to develop recommendations concerning effective physical activity policies and programs to address obesity in children. The conference was attended by approximately 1000 delegates from 33 countries who gathered to listen to the invited speakers and to share information on promising practices related to the promotion of physical activity with the aim of reducing the burden of obesity in children. The major topics addressed at the conference included the biological and behavioural causes of obesity, current and past levels of physical activity and sedentarism in children, the role of the social, family, and built environments in addressing the physical activity deficit, and the role of legislation and industry in promoting physical activity. Promising physical activity interventions among children were presented, and important research, policy, and practice recommendations to address the issue of physical inactivity and obesity were provided.
A pediatric perspective on inflammation and oxidative stress in response to exercise
Authors: Timmons Brian W. , Raha Sandeep 
Published: April 2008

Dr. Oded Bar-Or was a giant in the area of pediatric exercise science and made numerous contributions to the health of children around the world. He also had a significant impact on a relatively new area of pediatric exercise science — pediatric exercise immunology. Under his supervision, some aspects of the immunobiology of exercise have been unravelled from a pediatric perspective. In general, healthy children experience less inflammatory stress in response to standardized exercise, while demonstrating an exercise-induced elevation in the anabolic cytokine IL-8. Mechanisms for the maturity- and age-dependent changes in cytokine responses to exercise are unknown, but may involve oxidative stress. Studies of oxidative stress and exercise in children are scant, but preliminary data suggests that, like the inflammatory cytokine response to exercise, children may experience less oxidative stress in response to intense exercise. We propose that the links between exercise, inflammatory and oxidative stress, and growth factors will be important to understand in the context of how exercise contributes to optimal growth and development during childhood. This understanding will be particularly relevant for childhood diseases that restrict growth.
Reproducibility of short-term food intake and subjective appetite scores after a glucose preload, ventilation threshold, and body composition in boys
Authors: Bellissimo Nick , Thomas Scott G. , Pencharz Paul B. , Goode Robert C. , Anderson G. Harvey 
Published: April 2008

The objective of these studies was to assess the reproducibility of (i) short-term food intake (FI) and subjective appetite following a glucose preload, (ii) ventilation threshold (VT) and subjective appetite after short-duration exercise, and (iii) body composition assessed by bioelectrical impedance (BIA). On two separate weekend mornings, boys (n = 11; aged 9–14 years) received drinks containing 50 g glucose made up to 250 mL with water 2 h after a standardized breakfast. FI from a pizza meal was measured 30 min later. Subjective appetite was measured before and after the glucose loads and the VT measures. VTs were measured on 2 weekday evenings, 1 week apart. BIA was measured during the FI assessment sessions. Short-term FI after the glucose preload was highly reproducible. Mean energy intake was 925 ± 139 kcal on the first day and 988 ± 147 kcal on the second day (coefficient of repeatability (CR) = 259 kcal; intra-class correlation coefficient (ICC) = 0.96). Moderate reproducibility of the average appetite score was found at 30 min (CR = 24 mm; ICC = 0.82). Subjective appetite was increased similarly by short-duration exercise on both days (CR = 19 mm). Absolute VT was more highly reproducible (CR = 359 mL O2·min–1, ICC = 0.85) than VT expressed on the basis of body weight (CR = 8.0 mL O2·kg–1·min–1, ICC = 0.59). Fat mass (FM) estimated from BIA was highly reproducible (CR = 2.7 kg, ICC = 0.95), but underestimated FM compared with skinfolds. In conclusion, FI and subjective appetite scores in response to glucose preloads, VT, subjective appetite after short-duration exercise, and estimates of FM from BIA are reproducible in boys.
Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps
Authors: Boinapally Kavitha , Jiang Xiuping 
Published: April 2008

The objective of this study was to assess and differentiate wild-caught South Carolina (SC) shrimps from imported shrimps on the basis of microbiological analysis. Seven wild-caught SC shrimp and 13 farm-raised imported shrimp samples were analyzed. Total plate counts from wild-caught shrimp samples ranged from 4.3 to 7.0 log10 CFU/g, whereas counts from imported shrimp samples ranged from 3.2 to 5.7 log10 CFU/g. There was no difference (P > 0.05) between total bacterial counts of wild-caught SC shrimp and farm-raised imported shrimp. However, the percentages of bacteria with reduced susceptibility towards ceftriaxone and tetracycline were higher (P < 0.05) for farm-raised shrimp than for wild-caught samples. Salmonella spp. detected only in one farm-raised sample was resistant to ampicillin, ceftriaxone, gentamicin, streptomycin, and trimethoprim. Vibrio vulnificus was detected in both wild-caught and farm-raised shrimp samples; however, only the isolate from farm-raised shrimp was resistant to nalidixic acid and trimethoprim. Escherichia coli detected in one wild-caught sample was resistant to ampicillin. Both Listeria spp. and Salmonella spp. were absent with wild-caught SC samples. Therefore, the presence of more ceftriaxone- and tetracycline-resistant bacteria and the observed antimicrobial resistance phenotypes of isolates from the imported shrimp may reflect the possible use of antibiotics in raising shrimp in those countries.
1-Aminocyclopropane-1-carboxylate deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt
Authors: Cheng Zhenyu , Park Eunmi , Glick Bernard R. 
Published: April 2008

The growth of canola plants treated with either wild-type Pseudomonas putida UW4 or a 1-aminocyclopropane-1-carboxylate (ACC) deaminase minus mutant of this strain was monitored in the presence of inhibitory levels of salt, i.e., 1.0 mol/L at 10 °C and 150 mmol/L at 20 °C. This strain is psychrotolerant with a maximal growth rate of approximately 30 °C and the ability to proliferate at 4 °C. Although plant growth was inhibited dramatically by the addition of 1.0 mol/L salt at 10 °C and only slightly by 150 mmol/L salt at 20 °C under both sets of conditions, the addition of the wild type but not the mutant strain of P. putida UW4 significantly improved plant growth. This result confirms the previous suggestion that bacterial strains that contain ACC deaminase confer salt tolerance to plants by lowering salt-induced ethylene synthesis.
Prolactin Does Not Require Insulin-Like Growth Factor Intermediates but Synergizes with Insulin-Like Growth Factor I in Human Breast Cancer Cells
Authors: Carver Kristopher C. , Schuler Linda A. 
Published: April 2008

Abstract
Insulin-like growth factor (IGF)-II is a required intermediate for prolactin-induced up-regulation of cyclin D1 and proliferation in normal murine mammary epithelial cells in vivo and in vitro. However, we have recently shown that prolactin can rapidly induce cyclin D1 protein expression and subsequent proliferation in the MCF-7 human breast cancer cell line, suggesting that prolactin actions can be independent of IGFs in breast disease. Here, we investigate the relationship between these factors and show that prolactin up-regulated transcript levels of both IGF-I and IGF-II, but only after increases in cyclin D1 protein were observed. Moreover, prolactin increased cyclin D1 in the presence of the IGF-I receptor neutralizing antibody αIR3. However, on cotreatment, IGF-I and prolactin elicited cooperative phosphorylation of extracellular signal–regulated kinases 1 and 2 and protein kinase B/AKT, but not signal transducer and activator of transcription 5. This interaction extended to increased activation of activating protein-1 enhancer elements, phosphorylation of glycogen synthase kinase 3β, induction of cyclin D1, and ultimately, increased cell number. It also increased invasive behavior, which correlated with elevated matrix metalloproteinase-2 transcript levels. Interestingly, prolactin augmented phosphorylation at Tyr1135 and Tyr1136 of IGF-I receptor on cotreatment with IGF-I, although prolactin alone had no effect. Together, these data indicate that strong cooperative cross talk between prolactin and IGF-I augments biological processes associated with neoplastic progression, with implications for therapeutic strategies. (Mol Cancer Res 2008;6(4):634–43)
The direct thrombin inhibitor hirudin
Authors: Warkentin Theodore , Greinacher Andreas 
Published: April 2008

SummaryThis review discusses the pharmacology and clinical applications of hirudin, a bivalent direct thrombin inhibitor (DTI). Besides the current major indication for hirudin – anticoagulation of patients with heparin-induced thrombocytopenia (HIT) – the experience with hirudin in other indications, especially acute coronary syndromes, are briefly presented. Hirudins have been formally studied prior to their regulatory approval; however, important information on their side effects and relevant preventative measures only became available later. Therefore, current recommendations and dosing schedules for hirudin differ considerably from the information given in the package inserts. Drawbacks of hirudin and important precautions for avoiding potential adverse effects are discussed in detail in the third part of this review.
Venous and arterial thromboembolism in severe sepsis
Authors: LeClerc Jacques , Bailey Joan , Monberg Matthew , Sarwat Samiha , Levine Robert 
Published: April 2008

SummaryThe burden of thromboembolism (TE) in severe sepsis is largely unknown. We assessed the prevalence of venous and arterial TE in patients with severe sepsis over a four-week period. We performed a retrospective analysis of a pooled database of three randomized, placebo-controlled trials of two novel pharmacological agents for the treatment of severe sepsis, drotrecogin alfa (activated) (DrotAA)and secretory phospholipase A2 inhibitor (sPLA2I). The study was conducted at intensive care units of the participating institutions. A total of 2,649 patients with known or suspected infection and sepsis-associated acute organ dysfunction were enrolled in the three trials and were assigned to treatment groups (DrotAA=850; sPLA2I =578; placebo=1221). The database was queried for venous and arterial TE, using investigator reports of serious adverse events. Eighty-four of 2,649 patients (3.2%; 95% confidence interval, 2.5% to 3.9%) developed at least one thromboembolic event over 28 days. Nearly threequarters of episodes were atheroembolic (n=62); 25% involved the deep venous system (n=25). Ischemic stroke (n=30) and venous thromboembolism (n=25) each occurred in about 1% of patients. Ischemic stroke and acute coronary syndrome had a higher peak incidence during the first five days compared to venous TE onset, which was more constant over the 28-day period. Subgroup analysis by pooled treatment groups yielded TE rates of 2.0% (DrotAA), 3.5% (placebo), and 4.0% (sPLA2I), respectively. Clinically manifest TE occurred in about 3% of severe sepsis patients treated in the intensive care unit over a 28-day period. Arterial TE may be more common than previously recognized. More accurate estimates of TE prevalence and relationship to sepsis await future studies.
The musculus depressor mandibulae complex in frogs, with special remarks on ranids
Authors: Hoyos Julio Mario 
Published: April 2008

AbstractThe musculus depressor mandibulae is reviewed in frogs, with special consideration on species of the family Ranidae. Five character states of Starrett's classification of m. depressor mandibulae were found in frogs examined for this study, three of them in ranids. The non-existence of a true pars medialis, and modification of the muscle nomenclature are discussed. Based upon the nomenclature proposed by Edgeworth, the mm. depressor mandibulae externus and internus are reintroduced. Due to the multistate characters identified and the impossibility to establish their polarity the m. depressor mandibulae complex provides little or no phylogenetic information.
Estimation of Incident Photosynthetically Active Radiation from GOES Visible Imagery
Authors: Zheng Tao , Liang Shunlin , Wang Kaicun 
Published: April 2008

Abstract
Incident photosynthetically active radiation (PAR) is an important parameter for terrestrial ecosystem models. Because of its high temporal resolution, the Geostationary Operational Environmental Satellite (GOES) observations are very suited to catch the diurnal variation of PAR. In this paper, a new method is developed to derive PAR using GOES data. What makes this new method distinct from the existing method is that it does not need external knowledge of atmospheric conditions. The new method retrieves both atmospheric and surface conditions using only at-sensor radiance through interpolation of time series of observations. Validations against ground measurement are carried out at four “FLUXNET” sites. The values of RMSE of estimated and ground-measured instantaneous PAR at the four sites are 130.71, 131.44, 141.16, and 190.22 μmol m−2 s−1, respectively. At the four validation sites, the RMSE as the percentage of estimated mean PAR value are 9.52%, 13.01%, 13.92%, and 24.09%, respectively; the biases are −101.54, 16.56, 11.09, and 53.64 μmol m−2 s−1, respectively. The independence of external atmospheric information enables this method to be applicable to many situations in which external atmospheric information is not available. In addition, topographic impacts on surface PAR are examined at the 1-km resolution at which PAR is retrieved using the GOES visible band data.
Transient Analysis of Cold Winds on Exposed Skin: Reflections on the Assessment of Wind Chill Equivalent Temperatures
Authors: Shitzer Avraham 
Published: April 2008

Abstract
A transient analysis of the human–environment thermal interaction in cold and windy environments is presented. The site selected to represent this interaction is the head–face, which is depicted as a hollow cylinder wherein heat is conducted in the radial direction. Environmental radiation effects, which are small relative to the wind-driven convection effects, as well as metabolic heat and blood perfusion effects, are not accounted for at this stage. The model is solved analytically by an integral transform. Results are used to calculate the wind chill equivalent temperatures (WCETs) for different environmental temperatures and wind speeds. The temporal variations of the calculated WCETs are contrasted against the steady-state values that are published by the weather services in North America. The inherently transient nature of this parameter is clearly demonstrated. This observation should form a basis for modifying the heretofore employed steady-state calculation method of this useful weather predictor.
Streamflow Characteristics and Changes in Kolyma Basin in Siberia
Authors: Majhi Ipshita , Yang Daqing 
Published: April 2008

Abstract
This study documents major changes in streamflow hydrology over the Kolyma watershed due to climatic variations and human impacts. Streamflow seasonal cycles over the basin are characteristic of the northern region, with the lowest runoff in April and peak flow in June. Analyses of monthly flows and trends show that reservoir construction and operation have considerably affected streamflow regimes. Comparisons of mean monthly discharge records between pre- and post-1986 dam periods indicate that the mid–lower basin (downstream of the dam) experienced significant increase in low flows and decrease in peak flows after dam construction. For example, mean monthly flows during the post-dam period at the Ust’-Srednekan station (located 1423 km downstream of the dam) has strongly increased by about 205 m3 s−1 (or 522%–3157%) during December–April, and decreased by 133 m3 s−1 (41%) in June. Long-term monthly discharge data reveal an overall increase in streamflow during low flow seasons; the increase is greater for the stations located downstream of the dam. The Srednekolunsk station (1720 km from dam) shows low flow increase ranging from 130 (43%) to 268 m3 s−1 (454%) during November–April, and high discharge decrease by 2550 to 519 m3 s−1 during June–August in the post-dam era (1986–2000). These changes in flow patterns are mainly caused by reservoir regulation, as reservoirs release water in winter for power generation and store water in summer for flood control. Dam impact on flow regimes and changes are visible along the main river trunk; thus, the cold season discharge increase at the basin outlet is primarily the result of reservoir regulation. Annual discharge records show different changes within the Kolyma basin, with moderate increases in the upper basin and weak decreases in the mid–lower basin. Overall annual discharge near the basin outlet has decreased by 1.5% during 1978–2000. This study emphasizes the importance of human activities (particularly reservoirs) on seasonal and regional hydrology changes and points to the need to further examine natural causes and human impacts over other high-latitude watersheds.