bluuebunny/crossref_metadata_2025_split huggingface dataset:
Counting and coding the past: Circumscribing the variable context in quantitative analyses of past inflection
Authors: Hackert Stephanie 
Published: April 2008

AbstractAccurate circumscription of the variable context is crucial to any quantitative analysis of linguistic variation. Investigations of past inflection in African American Vernacular English and Caribbean English creoles thus generally include a more or less detailed section concerning the inclusion or exclusion of particular forms; the theoretical grounds on which these decisions are made, however, are not always spelled out. Consequently, there still does not seem to be agreement on what precisely constitutes the envelope of variation in such investigations—a fact that not only complicates data extraction and analysis but also hampers cross-variety comparisons. This article summarizes and evaluates previous definitions of the scope and relevant contexts of the variable (ed), providing internal (linguistic) argumentation supporting or contraindicating the inclusion or exclusion of particular tokens. My data stem from a larger study of past temporal reference in the urban variety of Bahamian Creole English (Hackert, Stephanie. [2004].Urban Bahamian Creole: System and variation. Amsterdam: Benjamins), an intermediate creole with close historical links with Gullah as well as relations with African American Vernacular English, Trinidadian Creole, and Barbadian.
Sociolinguistic patterns in grammaticalization:He, they, andthosein human indefinite reference
Authors: Laitinen Mikko 
Published: April 2008

AbstractSociolinguistic patterns in language change are largely based on generalizations from linguistic variables consisting of lexemes or morphemes. This article takes a diachronic, corpus-based approach to the diffusion of a change in a more extensive morphosemantic function consisting of several linguistic subsystems. It focuses on the pronoun variantshe, they, andthoseused for human indefiniteness in two contexts: (a) epicene anaphoric uses with indefinite pronouns and (b) cataphoric personal references. The quantitative corpus analyses show that the pronoun selection in Early and Late Modern English developed a greater tendency to use one pronoun type over the other in both contexts. The main data come from theCorpus of Early English Correspondenceand itsExtension. Statistical analyses compare the observed correlations of the pronouns with a set of social, external variables and language-internal factors. This article concludes that it is possible to establish sociolinguistic patterns in larger shifts if we account for the closely related internal developments in the language.
Regional differences in perceiving vowel tokens on Southerness, education, and pleasantness ratings
Authors: Fridland Valerie 
Published: April 2008

AbstractThis study investigates the role of regional dialect experience on the social awareness of synthesized vowel tokens to regional in-group and out-group members. For the study, speakers from Reno, NV, were given the same perception test used in a previous study in Memphis, TN. Comparing the Reno results to those found in Memphis, the study examines whether differences in regional vowel norms affect how Westerners rate Southern-shifted and non-Southern-shifted vowel variants on Southernness, education, and pleasantness scales. The study also looks at how Reno raters interpreted shifted back vowel variants, found productively in their local community, compared to front vowel shifts found exclusively in the South. Finally, the paper explores how the results suggest that regional dialect exposure attunes listeners to attend to different aspects of vowel quality than those outside the region. In examining how regional dialect experience affects listener recognition and evaluation of local and nonlocal vowel norms, the paper begins to explore how much the production/perception relationship is mediated by speakers' participation in locally constructed and defined speech communities.
The role of prosody in morphological change: The case of Hebrew bound numerals
Authors: Meir Irit 
Published: April 2008

AbstractThe morphological system of cardinal numerals in Modern Hebrew is currently undergoing rapid changes, enabling linguists to unravel the forces shaping the change as it takes place. In the free forms, gender marking on numerals is neutralized by collapsing both masculine and feminine forms into one paradigm, the feminine paradigm. In the bound (definite) forms, an opposite direction is attested, in that at least for some numerals, the masculine forms become more prevalent. The study reported here aims to determine whether the factor determining the change is prosodic or functional in nature, by eliciting production and grammaticality judgments of noun phrases containing bound numerals from five different age groups of native speakers. The results suggest that prosody plays a role in shaping the change, as forms with penultimate stress are favored over those with ultimate stress. In addition, processes of production and processes of grammaticality judgments seem to be subject to different kinds of constraints. This state of affairs indicates that the tension between the tendencies toward simplification on the one hand and maximal distinctness on the other occurs at the morphological level as well.
Effect of cytochalasins B and D on the developmental competence of somatic cell nuclear transfer embryos in miniature pigs
Authors: Sugimura Satoshi , Kawahara Manabu , Wakai Takuya , Yamanaka Ken-ichi , Sasada Hiroshi , Sato Eimei 
Published: April 2008

SummaryIn many animals, cytochalasins have generally been used as cytoskeletal inhibitors for the diploid complement retention of somatic cell nuclear transfer (SCNT) embryos. However, limited information is available on the effects of cytochalasins on the in vitro development of SCNT embryos. Hence, we compared the effects of cytochalasin B (CB) and cytochalasin D (CD) on pseudo-polar body (pPB) extrusion, cortical actin filament (F-actin) distribution in porcine parthenogenetic oocytes and in vitro development of SCNT embryos that were reconstructed using foetal fibroblasts in the G0/G1 phase derived from miniature pigs. CB (7.5 μg/ml) and CD (2.5 μg/ml) treatments effectively inhibited pPB extrusion in SCNT embryos. CB (2.5 μg/ml) treatment could not inhibit pPB extrusion and insufficiently destabilized F-actin immediately following artificial activation. In parthenogenetic oocytes treated with 2.5 μg/ml CD, normal reorganization and uniform distribution of cortical F-actin at the cytoplasmic membrane were observed at 8 h after artificial activation; this finding was similar to that of control oocytes. In contrast, parthenogenetic oocytes treated with 7.5 μg/ml CB showed non-uniform distribution of F-actin at 8 h after artificial activation. On day 5 after in vitro cultivation, the blastocyst formation rate of SCNT embryos treated with 2.5 μg/ml CD was significantly higher than that of SCNT embryos treated with 2.5 and 7.5 μg/ml CB (p < 0.05). Hence, the present findings suggest that CD is more effective than CB as the cytoskeletal inhibitor for the production of SCNT embryos in miniature pigs.
Ultrastructural changes in goat interspecies and intraspecies reconstructed early embryos
Authors: Tao Yong , Cheng Lizi , Zhang Meiling , Li Bin , Ding Jianping , Zhang Yunhai , Fang Fugui , Zhang Xiaorong , Maddox-Hyttel Poul 
Published: April 2008

SummaryThe low efficiency of somatic cell nuclear transfer may be related to the ultrastructural deviations of reconstructed embryos. The present study investigated ultrastructural differences between in vivo-produced and cloned goat embryos, including intra- and interspecies embryos. Goat ear fibroblast cells were used as donors, while the enucleated bovine and goat oocytes matured in vitro as recipients. Goat–goat (GG), goat–cattle (GC) and goat in vivo-produced embryos at the 2-cell, 4-cell, 8-cell and 16-cell stages were compared using transmission electron microscopy. These results showed that the three types of embryos had a similar tendency for mitochondrial change. Nevertheless, changes in GG embryos were more similar to changes in in vivo-produced embryos than were GC embryos, which had more extreme mitochondrial deviation. The results indicate the effects of the cytoplast on mitochondria development. The zona pellucida (ZP) in all three types of embryos became thinner and ZP pores in both GC and GG embryos showed an increased rate of development, especially for GC embryos, while in vivo-produced embryos had smooth ZP. The Golgi apparatus (Gi) and rough endoplasmic reticulum (RER) of the two reconstructed embryos became apparent at the 8-cell stage, as was found for in vivo embryos. The results showed that the excretion of reconstructed embryos was activated on time. Lipid droplets (LD) of GC and GG embryos became bigger, and congregated. In in vivo-produced embryos LD changed little in volume and dispersed gradually from the 4-cell period. The nucleolus of GC and GG embryos changed from electron dense to a fibrillo-granular meshwork at the 16-cell stage, showing that nucleus function in the reconstructed embryos was activated. The broken nuclear envelope and multiple nucleoli in one blastomere illuminated that the nucleus function of reconstructed embryos was partly changed. In addition, at a later stage in GC embryos the nuclear envelope displayed infoldings and the chromatin was concentrated, implying that the blastomeres had an obvious trend towards apoptosis. The gap junctions of the three types of embryos changed differently and GG and GC embryos had bigger perivitelline and intercellular spaces than did in vivo-produced embryos. These results are indicative of normal intercellular communication at an early stage, but this became weaker in later stages in reconstructed embryos. In conclusion, inter- and intraspecies reconstructed embryos have a similar pattern of developmental change to that of in vivo-produced embryos for ZP, rough ER, Gi and nucleolus, but differ for mitochondria, LD, vesicles, nucleus and gap junction development. In particular, the interspecies cloned embryos showed more severe destruction. These ultrastructural deviations might contribute to the compromised developmental potential of reconstructed embryos.
Bovine oocytes and early embryos express Staufen and ELAVL RNA-binding proteins
Authors: Calder M.D. , Madan P. , Watson A.J. 
Published: April 2008

SummaryRNA-binding proteins (RBP) influence RNA editing, localization, stability and translation and may contribute to oocyte developmental competence by regulating the stability and turnover of oogenetic mRNAs. The expression of Staufen 1 and 2 and ELAVL1, ELAVL2 RNA-binding proteins during cow early development was characterized. Cumulus–oocyte complexes were collected from slaughterhouse ovaries, matured, inseminated and subjected to embryo culturein vitro. Oocyte or preimplantation embryo pools were processed for RT-PCR and whole-mount immunofluorescence analysis of mRNA expression and protein distribution. STAU1 and STAU2 and ELAVL1 mRNAs and proteins were detected throughout cow preimplantation development from the germinal vesicle (GV) oocyte to the blastocyst stage. ELAVL2 mRNAs were detectable from the GV to the morula stage, whereas ELAVL2 protein was in all stages examined and localized to both cytoplasm and nuclei. The findings provide a foundation for investigating the role of RBPs during mammalian oocyte maturation and early embryogenesis.
Sex determining of cat embryo and some feline species
Authors: Ciani Francesca , Cocchia Natascia , Rizzo Maria , Ponzio Patrizia , Tortora Gennaro , Avallone Luigi , Lorizio Roberto 
Published: April 2008

SummarySex identification in mammalian preimplantation embryos is a technique that is used currently for development of the embryo transfer industry for zootechnical animals and is, therefore, a resource for biodiversity preservation. The aim of the present study was to establish a rapid and reliable method for the sexing of preimplantation embryos in domestic cats. Here we describe the use of nested PCR identify Y chromosome-linked markers when starting from small amounts of DNA and test the method for the purpose of sexing different species of wild felids. To evaluate the efficiency of the primers, PCR analysis were performed first in blood samples of sex-known domestic cats. Cat embryos were produced both in vitro and in vivo and the blastocysts were biopsied. A Magnetic Resin System was used to capture a consistent amount of DNA from embryo biopsy and wild felid hairs. The results from nested PCR applied on cat blood that corresponded to the phenotypical sex. Nested PCR was also applied to 37 embryo biopsies and the final result was: 21 males and 16 females. Furthermore, β-actin was amplified in each sample, as a positive control for DNA presence. Subsequently, nested PCR was performed on blood and hair samples from some wild felines and again the genotyping results and phenotype sex corresponded. The data show that this method is a rapid and repeatable option for sex determination in domestic cat embryos and some wild felids and that a small amount of cells is sufficient to obtain a reliable result. This technique, therefore, affords investigators a new approach that they can insert in the safeguard programmes of felida biodiversity.
Isolation of egg cells and zygotes of Torenia fournieri L. and determination of their surface charge
Authors: Chen S.H. , Yang Y.H. , Liao J.P. , Kuang A.X. , Tian H.Q. 
Published: April 2008

SummaryEgg cells of Torenia fournieri were isolated from embryo sacs 1 day after anthesis using enzymatic digestion or mechanical dissection. About 5% of the egg cells and zygotes (2–3 from 50 ovules) could be mechanically dissected within 2 h. When 0.1% cellulase and 0.1% pectinase were added to the mannitol isolation solution, about 18% of the egg cells (8–10 from 50 ovules) could be isolated within 2 h. The egg cells isolated by mechanical dissection could be used for in vitro fertilization studies without any of the potentially deleterious effects of the enzymes on the plasma membrane of egg cell. The egg cells isolated using enzymatic digestion could be used in the study of the molecular biology of female gamete because more egg cells could be isolated with this technique. Using enzymatic digestion, over 10 zygotes from 50 ovules (over 20%) were isolated from the pollinated ovules. Coupled with our successful isolation of mature sperm cells, the isolation of egg cells of T. fournieri will make in vitro fertilization possible in a dicotyledon plant.
Assessment of diastolic ventricular function in fetuses of diabetic mothers using tissue Doppler
Authors: Hatém Maria Amélia Bulhões , Zielinsky Paulo , Hatém Domingos Mohamad , Nicoloso Luiz Henrique , Manica João Luiz , Piccoli Antonio L. , Zanettini Juliana , Oliveira Vinicius , Scarpa Fernanda , Petracco Rafaella 
Published: April 2008

AbstractObjectiveTo identify the presence of ventricular diastolic dysfunction by tissue Doppler in fetuses of diabetic mothers, with or without septal hypertrophy, in comparison to fetuses of nondiabetic mothers.MethodsA contemporary transverse study in fetuses with a gestational age between 25 weeks to term, studying diastolic function by assessment using tissue Doppler and pulsed wave Doppler of the atrioventricular diastolic flow. The mothers of the fetuses all had previous or gestational diabetes, and were referred to the Fetal Cardiology Unit of the Institute of Cardiology in Porto Alegre, Brazil. We analysed variance with the Student-Neumann-Keuls post hoc test. An alfa of 0.05 was considered significant for statistical analysis.ResultsThe mean myocardial velocities of the E′and A′ waves at the mural mitral annulus, in fetuses of diabetic mothers with myocardial hypertrophy, were, respectively, 7.00 plus or minus 1.6 centimetres per second, and 10.24 plus or minus 3.3 centimetres per second. In the fetuses of diabetic mothers group without myocardial hypertrophy, the comparable values were 7.19 plus or minus 2.4 centimetres per second and 10.77 plus or minus 3.77 centimetres per second, respectively. In the control group, they were 4.81 plus or minus 0.85 centimetres per second and 8.01 plus or minus 2.2 centimetres per second. The difference between the velocities in fetuses of diabetic mothers and in fetal normal mothers was statistically significant (p less than 0.05). Statistically significant differences were also observed in E′ and A′ diastolic waves at the aortic mitral annulus, as well as for the tricuspid annulus when tissue Doppler assessment was carried out in the same sample. The mean ratio between the E and E′ of mitral and tricuspid waves in the control fetuses of normal mothers was significantly higher than in fetuses of diabetic mothers.ConclusionPulsed tissue Doppler, when used in fetuses of diabetic mothers and compared with fetuses of nondiabetic mothers, shows evidence of impaired diastolic function, independently of the presence of myocardial hypertrophy.
Differences according to gender in reporting physical symptoms during echocardiographic screening in healthy teenage athletes
Authors: Movahed Mohammad Reza , Martinez Adolfo , Morrell Holly , Greaves Seaneen , Greaves Jeff , Sattur Sudhakar 
Published: April 2008

AbstractBackgroundMany studies have suggested that more women then men present with physical symptoms. There is no data available, however, on the differences in reporting of physical symptoms between teenage male and female athletes. Our objective was to evaluate the differences according to gender in physical symptoms in healthy teenage athletes.MethodsA total of 1,465 high school athletes, between the ages of 13 and 19 years participated in a mass echocardiographic screening programme for detection of cardiac abnormalities. Screening was conducted using a hand-carried cardiac ultrasound device (OptiGo, Philips). All participants were actively involved in a high school sport programme. Each athlete was required to fill out a questionnaire before the screening. The athletes were asked to report the occurrence of physical symptoms with activity or exercise. A physical examination was not performed during screening.ResultsThere were 1,031 (70.4%) male and 434 (29.6%) female participants. Significantly more female teenage athletes reported physical symptoms (190/434, 43.8% versus 267/1,031, 25.9%, odds ratio: 2.28, confidence interval: 1.76–2.81, p less than 0.001). Symptoms did not correlate with any echocardiographically identified cardiac abnormalities in either gender. The differences in the reporting of symptoms were significant for all physical symptoms addressed by the questionnaire.ConclusionThere is a high prevalence of reporting physical symptoms in young healthy athletes without any relation to cardiac abnormalities. Young female athletes report physical symptoms nearly twice as often as their male counterparts.
Paediatric cardiac assistance in developing and transitional countries: the impact of a fourteen year effort
Authors: Novick William M. , Stidham Gregory L. , Karl Tom R. , Arnold Robert , Anić Darko , Rao Sri O. , Baum Victor C. , Fenton Kathleen E. , Di Sessa Thomas G. 
Published: April 2008

AbstractBackgroundPaediatric cardiac services are poorly developed or totally absent in underdeveloped countries. Institutions, foundations and interested individuals in those nations in which sophisticated paediatric cardiac surgery is practised have the ability to alleviate this problem by sponsoring paediatric cardio-surgical missions to provide care, and train local caregivers in developing, transitional, and third world countries. The ultimate benefit of such a programme is to improve the surgical abilities of the host institution. The purpose of this report is to present the impact of our programme over a period of 14 years.MethodsWe specifically reviewed our database of patients from our missions, our team lists, surgical results, and the number and type of personnel trained in the institutions that we have assisted. In order for the institution to be entered into the study, the foundation had to provide at least 2 months of training. In addition, the institution had to respond to a simple questionnaire concerning the number and types of surgery performed at their facility before and after intervention by the foundation.ResultsWe made 140 trips to 27 institutions in 19 countries, with 12 of the visited institutions qualifying for inclusion. Of these, 9 institutions reported an increase in the number and complexity of cases currently being performed in their facility since the team intervened. This goal had not been accomplished in 3 institutions. The reasons for failure included the economic situation of the country, hospital and national politics, personality conflicts, and continued lack of hardware and disposables.ConclusionsPaediatric cardiac service assistance can improve local services. A significant commitment is required by all parties involved.
Off-pump connection of the hepatic to the azygos vein through a lateral thoracotomy for relief of arterio-venous fistulas after a Kawashima procedure
Authors: Lopez Fatima E.F.G. , van den Heuvel Freek , Pieper Petronella G. , Waterbolk Tjalling W. , Ebels Tjark 
Published: April 2008

AbstractObjectiveTo connect the hepatic veins to the azygos venous system through a lateral thoracotomy, and without the use of extracorporeal circulation, so as to relieve arteriovenous fistulas after a previous Kawashima operation.MethodsDescription of the operative technique by which the hepatic veins are anastomosed to the hepatic venous system.ResultsThe surgical approach was successfully applied in 3 patients, all of whom showed an excellent rise of saturations of oxygen after redirection of the hepatic venous blood.ConclusionThe operative method presented is an elegant means of redirecting the hepatic venous blood to the pulmonary circulation without the disadvantages of extracorporeal circulation and resternotomy.
The Zeroing Issue: a critical analysis of Softwood V
Authors: BOWN CHAD P. , SYKES ALAN O. 
Published: April 2008

AbstractThis paper addresses the issues that came before the Appellate Body in the Softwood V dispute, concerning an affirmative antidumping determination by the US Department of Commerce. The paper addresses both the original Appellate Body opinion in the dispute, and the later opinion reviewing the compliance panel findings. We focus primarily on the ‘zeroing’ issue in ‘transaction-to-transaction (T–T)’ calculations of dumping, and briefly on two other cost-allocation issues. In general, we are ambivalent about the Appellate Body's approach to the zeroing issue. On the one hand, zeroing inflates dumping margins without any sound economic rationale for doing so. On the other hand, zeroing has been a standard administrative practice for many years and the ADA does not clearly prohibit it. The Appellate Body's legal analysis of the matter in T–T cases, in particular, rests on shaky premises. We also consider the wisdom of addressing the zeroing issue in piecemeal fashion through what has proven to be a lengthy sequence of narrow decisions.