bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative studies on the expression of tumor‐associated glycoprotein (TAG‐72), CA 19‐9 and DU‐pan‐2 in normal, benign and malignant pancreatic tissue
Authors: Takasaki Hideaki , Tempero Margaret A. , Uchida Eiji , Büchler Markus , Ness Marsha J. , Burnett David A. , Metzgar Richard S. , Colcher David , Schlom Jeffrey , Pour Parviz M. 
Published: February 2007

AbstractExpression of tumor‐associated glycoprotein (TAG‐72) was examined by immunohistochemistry in pancreatic specimens from normal donors, and from patients with chronic pancreatitis and pancreatic cancer, and was compared with expression of CA 19‐9 and DU‐PAN‐2. In the normal pancreas, TAG‐72 was expressed in fewer ductal and ductular cells than were CA 19‐9 (p < 0.05) and DU‐PAN‐2 (P < 0.01 and 0.001 respectively), whereas in chronic pancreatitis all 3 antigens were expressed in ductal cells but only CA 19‐9 and DU‐PAN‐2 in ductular cells (p <0.001). In the specimens from normal pancreas and chronic pancreatitis cases, TAG‐72 was localized in the Golgi region, whereas CA 19‐9 and DU‐PAN‐2 showed diffuse cytoplasmic and glycocalyx patterns. In pancreatic cancer, although the rate of expression of all 3 antigens was similar, their cellular localization differed significantly: only TAG‐72 was expressed in the Golgi region (p < 0.001), whereas CA 19‐9 showed mainly glycocalyx (p < 0.05) and/or intra‐luminal patterns (p <0.01) compared with that of the other 2 antigens. We conclude that, due to differences in expression of TAG‐72 in benign versus malignant cells, the monoclonal antibody against TAG‐72 (B72.3) may be suitable for radioimmunodetection or radio‐immunotherapy of pancreatic cancer.
c‐fos proto‐oncogene expression in human NK/LGL cells: Expression is not constitutive and is associated with functional activation
Authors: Colotta Francesco , Polentarutti Nadia , Pirelli Anna , Dibello Maria , Allavena Paola , Mantovani Alberto 
Published: February 2007

AbstractUnlike mature myeloid and monocytic cells and cell lines committed to terminal myelomonocytic differentiation, highly purified preparations of human natural killer cells/large granular lymphocytes (NK/LGL) did not spontaneously express the c‐fos proto‐oncogene. Transient expression of c‐fos in NK/LGL was associated with functional activation with IL‐2, IFN‐γ, LPS and PMA, which increased their cytotoxic activity, lymphokine secretion and in vitro chemotaxis. These results, together with the finding that T‐cell receptor genes are in germ‐line configuration in the vast majority of peripheral blood NK/LGL, are compatible with the hypothesis that these cells represent a separate hematopoietic lineage.
Relations between ph, oxygen partial pressure and growth in cultured cell spheroids
Authors: Carlsson Jörgen , Acker Helmut 
Published: February 2007

AbstractThe PH gradients, oxygen partial‐pressure gradients and growth curves were measured for 7 different types of spheroids. Growth curves were measured in liquid overlay culture and thereafter the spheroids were attached to cover glasses and transferred to a chamber for micro‐electrode measurements. The spheroids were randomly divided for PH or pO2 measurements which then were made under conditions as identical as possible. The decreases in pO2 and pH, ΔpO2 and ΔPH were calculated as the difference between the values in the culture medium and the values 200 μm inside the spheroids. Each type of spheroid had a certain relation between ΔpO2 and ΔpH. The human colon carcinoma HT29, the mouse mammary carcinoma EMT6 and the hamster lung V79‐379A spheroids had high values of the quotient ΔpO2/ΔpH. The human thyroid carcinoma HTh7 spheroids and the 3 types of human glioma spheroids had lower quotients. There was a tendency for fast‐growing spheroids to have high quotients. Two extreme types of spheroids, HT29 (high quotient) and UII8 MG (Iow quotient) were analyzed for lactate production and oxygen consumption. The U‐II8 MG spheroids produced about 3 times more lactate and consumed about 3 times less oxygen than the HT29 spheroids. The differences in lactate production could not be explained by differences in the pyruvate Km values of lactate dehydrogenase. The results indicate that there are significant metabolic differences between the spheroid systems studied.
Increased formation of unintegrated viral DNA in skin fibroblasts from patients with familial polyposis coli shortly after infection with kirsten murine sarcoma virus
Authors: Miyaki Michiko , Tanaka Kiyoko , Sato Chicko , Akamatsu Noriko , Mori Takeo , Tsuchida Nobuo 
Published: February 2007

AbstractSkin fibroblasts from patients with familial polyposis coli (FPC) exhibit increased frequency of morphological transformation, anchorage‐independent growth, and enhanced production of viral RNA and viral particles after infection with murine sarcoma virus(murine leukemia virus), MSV(MLV). These FPC fibroblasts form greater amounts of unintegrated viral DNA as compared to normal fibroblasts during the first 4 to 24 hr after infection with Kirsten MSV(KiMLV), suggesting that increased susceptibility of FPC cells to MSV is determined at an early stage of viral transformation. Several MSV‐transformed clones of both FPC and normal fibroblasts, isolated from agarose gel, were non‐producers having integrated viral genome which was expressed into viral RNA and rescued by infection with MLV.
The role of polymorphonuclear leukocytes (PMN) on the growth and metastatic potential of 13762nf mammary adenocarcinoma cells
Authors: Aeed Paul A. , Nakajima Motowo , Welch Danny R. 
Published: February 2007

AbstractCirculating neutrophil (PMN) levels can increase in rats bearing subcutaneously growing clones of the 13762NF mammary adenocarcinoma and the level of increase correlates with the metastatic potential of the clone. In rats with poorly metastatic MTC tumors, numbers of circulating PMN did not rise, whereas PMN levels rose 50‐fold in rats bearing highly metastatic MTLn3, 12‐fold in rats with weakly metastatic MTLn2, and 14‐fold in those with moderately metastatic MTF7 tumors. Neutrophilia was caused partly by tumor size, but metastatic potential was a stronger determinant, suggesting that PMNs may play a role in the metastatic process. To determine whether circulating PMNs indeed contribute to cellular metastatic potential, we examined effects of PMN on various aspects of the metastatic process. Experimental metastasis assays involving i. v. co‐injections of PMNs yielded a dose‐dependent increase in extrapulmonary metastases for MTLn3, but no change in lung colonization potential for any of the clones examined. The change in the metastatic profile was not due to any modification in in vivo distribution of i. v. injected tumor cells or in adhesion to endothelial monolayers in vitro. PMNs also had no effect on in vitro DNA, RNA or protein synthesis and were not cytolytic (E:T 100: I). However, PMNs collected from high‐passage MTLn3 tumor‐bearing rats had a 50% increase in heparanase and type‐IV collagenolytic activity as compared to unstimulated PMNs isolated from normal rats. These results indicate that polymorphonuclear cells may contribute to the metastatic potential of highly metastatic clones from the 13762NF mammary adenocarcinoma cells by assisting in the degradation of basement membrane during extravasation.
Expression of the c‐Ha‐ras and neu oncogenes in DMBA‐induced, anti‐estrogen‐treated rat mammary tumors
Authors: Vuorio Tuula , Wärri Anni , Sandberg Minna , Alitalo Kari , Vuorio Eero 
Published: February 2007

AbstractDimethylbenzanthracene (DMBA)‐induced rat mammary tumors were analyzed for the structure and expression of the oncogenes c‐Ha‐ras and neu and the effects of anti‐estrogen treatment. Tumor samples were divided into 3 groups, the first consisting of untreated tumors, the second of anti‐estrogen (toremifene)‐treated unresponsive (growing) tumors, and the third of toremifene‐treated responsive (regressing) tumors. DNA and RNA derived from normal tissues of the same experimental animals were also analyzed. In Southern blot analysis of genomic DNAs, 2 tumors out of 23 contained new Xbal site in the Ha‐ras gene, indicating a point mutation in the second nucleotide of codon 61. Both of these tumors belonged to the group that had not received toremifene. No amplifications of the Ha‐ras or the neu genes were observed. Although greatly variable, the levels of Ha‐ras mRNA were highest in untreated tumors, lower in toremifene‐treated, unresponsive tumors and even lower in toremifene‐treated, regressing tumors, corresponding approximately to the levels detected in normal liver and uterus of untreated animals. Expression of the neu mRNA was variable and considerably lower than that of Ha‐ras mRNA. It was similar in all 3 groups and somewhat elevated than in several non‐malignant control tissues. Localization of c‐Ha‐ras expression by in situ hybridization revealed a relatively even distribution of the mRNA throughout the mammary tissue. The results suggest that mechanisms other than activation of the c‐Ha‐ras or neu genes are important for progression and regression of DMBA‐induced rat mammary carcinomas.
Effects of recombinant human tumor necrosis factor‐α on the surface phenotype and the growth of human malignant glioma cell lines
Authors: Zuber P. , Accolla R. S. , Carrel S. , Diserens A. C. , de Tribolet N. 
Published: February 2007

AbstractHuman malignant glioma cell lines and clones were incubated with various concentrations of recombinant human TNF‐α, either alone or in combination with recombinant human IFN‐γ. The surface expression of HLA‐ABC (class I) antigens and β2‐microglobulin, was significantly enhanced by TNF‐α alone on every cell line and clone tested. After incubation with both TNF‐α and IFN‐γ, the surface expression of HLA‐ABC antigens was only slightly higher than that observed with each cytokine alone. In contrast to IFN‐γ, TNF‐α had no effect on the surface expression of HLA‐DR (class II) antigens. Moreover, the surface expression of HLA‐DR induced by IFN‐γ was unaffected by TNF‐α. The increased expression of HLA‐ABC antigens after treatment with TNF‐α or IFN‐γ correlated with increased levels of HLA‐ABC‐specific mRNA. In addition, TNF‐α, like IFN‐γ, selectively enhanced the surface expression of a tumor‐associated antigen, Me14‐D12, while it had no effect on the expression of various other surface antigens. In the absence of actinomycin D, TNF‐α exhibited no direct cytotoxic/cytostatic effect on the glioma cell lines tested. These results indicate that TNF‐α can enhance the surface expression of HLA‐ABC antigens on human glioma cells in the absence of a direct cytotoxic/cytostatic effect.
Utilization of human hematopoietic cell lines for the propagation and characterization of HBLV (human herpesvirus 6)
Authors: Ablashi Dharam V. , Lusso Paolo , Hung Chia‐Ling , Salahuddin S. Zaki , Josephs Steven F. , Llana Ted , Kramarsky Bernhard , Biberfeld Peter , Markham Phillip D. , Gallo Robert C. 
Published: February 2007

AbstractDetails of the productive infection of established human cell lines of diverse origin by HBLV (also designated Human Herpesvirus 6) are described in this report. The infection and replication of HBLV in several T and B lymphoid and other cell lines was observed by electron microscopic examination, by the detection of viral antigen expression by indirect immunofluorescence assay (IFA) and by the presence of HBLV DNA by Southern blot hybridization. Several of these cell lines produced large amounts of virus. For this reason and because of the absence of other human herpesviruses, these lines have provided a valuable resource for the preparation of reagents and the development of assays for the detection and characterization of HBLV. The isolation and characterization of new HBLV isolates from patients with chronic fatigue syndrome were also facilitated by using some of the cell lines reported here. The host range of HBLV in established cell lines, therefore, does not appear to be limited to the B lymphocytes, as initially suggested by in vivo studies. The infection of T and B lymphocytes, megakaryocytes and neuronal cells in vitro suggests a need for the evaluation of diverse hematological and neurological disorders to shed light on a possible HBLV involvement.
Monoclonal antibodies against doxorubicin
Authors: Balsari Andrea , Alzani Rachele , Parrello Daniele , Morelli Daniele , Tagliabue Elda , Gianni Luca , Isetta Anna Maria , Menard Sylvie , Colnaghi Maria Ines , Ghione Mario 
Published: February 2007

AbstractHybridomas which secrete monoclonal antibodies (MAbs) reacting with doxorubicin (DXR), a widely used anthracycline cytotoxic antibiotic, have been obtained by fusing NSO/P3 mouse myeloma cells with spleen cells from BALB/c mice immunized with DXR‐BSA conjugate. The best producer among the several clones obtained was expanded and the secreted MAb (MAD2) purified and characterized. MAD2 cross‐reacts to varying degrees with anthracycline compounds such as some DXR analogues and derivatives, but does not recognize anthracene and anthraquinone structures, with the exception of weakly reacting Mitoxantrone. MAD2 and the panel of MAbs which are at present being purified may become a tool for studying the relevance of different domains of the anthracyclin molecule in terms of biologic activity.
Possible role of certain antioxidant enzymes in dimethy lnitrosamine‐induced liver carcinogenesis
Authors: Liotti F. S. , Menghini A. R. , Guerrieri P. , Mariucci G. , Locci P. , Bruschelli G. 
Published: February 2007

AbstractAn investigation was carried out to establish whether the reduction in catalase, glutathione peroxidase and superoxide dismutase activity, normally observed in liver tumours, is an early event and therefore of pathogenetic importance, or whether it is a late occurrence. Experiments performed on dimethylnitrosamine‐treated hepatectomized and non‐hepatectomized rats show that the decrease in activity of these enzymes is entirely due to hepatectomy, since the tumourinducing doses of dimethylnitrosamine failed to provoke variations in the activity of these enzymes, in either normal or regenerating liver.
Immunophenotypic similarities of mediastinal clear‐cell lymphoma and sinusoidal (monocytoid) b cells
Authors: Möller Peter , Matthaei‐Maurer D. Ulrike , Hofmann Walter J. , Dörken Bernd , Moldenhauer Gerhard 
Published: February 2007

AbstractUsing 14 well‐defined (clustered) monoclonal antibodies (MAbs) against B‐cell restricted/associated differentiation and activation (CD) antigens and 12 mediastinal clear‐cell lymphomas (MCCL), 46 follicular‐center‐cell lymphomas (FCCL), and 20 non‐neoplastic lymph nodes—including toxoplasmic and HIV‐associated lymphadenitis—were immunohistochemically examined to determine the histogenesis of MCCL. Antigenically, MCCL was characterized as CD5−, CD10−, CD19+, CD20+, CD21−, CD22+, CD30−, CD37+, CDw40+, and by a frequent expression of CD11c and CD23, while other antigens were inconsistently expressed. The antigenic profiles of MCCL and FCCL showed statistically significant differences in 4/14 distinct antigens. When the neoplastic cells of both tumor groups were compared with morphologically defined normal B‐cell types, the overall resemblance of their immunophenotypes was even closer between MCCL and sinusoidal (monocytoid) B cells than between FCCL and follicular‐center B cells. We conclude that MCCL is a lymphoma type distinct from FCCL, most probably representing a highly malignant neoplasm corresponding to sinusoidal B‐cell reaction.
Elevated cerebrospinal fluid fibronectin concentration at diagnosis indicates poor prognosis in children with acute lymphoblastic leukemia
Authors: Rautonen Jukka , Koskiniemi Marjaleena , Siimes Martti A. , Salonen Eeva‐Marjatta , Vaheri Antti 
Published: February 2007

AbstractWe investigated whether the concentration of fibronectin (FN) in the cerebrospinal fluid (CSF) could be used for identifying patients with subclinical blast‐cell infiltration in the central nervous system (CNS) and an increased risk of CNS relapse later in the course of their leukemia. Our series comprised 36 children with newly diagnosed acute lymphoblastic leukemia (ALL). The mean follow‐up time was 54 months (range 5 to 96 months). The median concentration of CSF‐FN at diagnosis was 2.24 mg/l (range 0.78 to 7.04 mg/l). The 3‐year continuous complete remission rate for the 16 patients with CSF‐FN concentrations <2mg/l was 93% as compared with 37% for the 19 patients with CSF‐FN >2mg/l (p = 0.001). When multivariate analysis was performed, the CSF‐FN concentration retained its prognostic significance. When all relapses were considered as failures, the relative risks of relapse for patients with CSF‐FN <2mg/l and >2mg/l were I and 15.8 (95% confidence limits 1.8‐135.6, p<0.02), respectively. If only CNS relapses (isolated and combined) were considered as failures, relative risks for the above‐mentioned groups were I and 11.6 (1.4‐99.5, p<0.05), respectively. We conclude that determination of the CSF‐FN concentration may provide a new means of evaluating the CSF in children with ALL and may prove to be a sensitive indicator of leukemic CNS infiltration.
Detection of iga antibodies against human papillomavirus in cervical secretions from patients with cervical intraepithelial neoplasia
Authors: Dillner Lena , Bekassy Zolian , Jonsson Nils , Moreno‐Lopez Jorge , Blomberg Jonas 
Published: February 2007

AbstractAlthough human papillomavirus (HPV) is involved in the etiology of cervical carcinoma, there are no cervical carcinoma‐specific HPV serologic tests available. We investigated the presence of broadly cross‐reactive IgA antibodies to papillomavirus (PV) in cervico‐vaginal secretions from patients with condylomata and cervical intraepithelial neoplasia (CIN). Purified bovine PV (BPV) virions were used as antigen in an enzyme‐linked immunosorbent assay (ELISA). Forty‐two women whose ages ranged from 20 to 50 participated in the study. Eight of 9 patients with CIN had IgA antibodies against PV in their cervical secretions. Three of 9 patients with koilocytosis and condylomas but no CIN had IgA antibodies to PV. Six of 24 women with normal Pap‐smear and colposcopy also had IgA antibodies against PV in their cervical secretions. The proportion of IgA‐positive cervical secretions was significantly higher in the CIN group than in the normal group (p<0.005). Our data suggest that IgA antibodies to PV may be a useful marker for CIN.
Epstein‐barr virus‐infected b cells persist in the circulation of acyclovir‐treated virus carriers
Authors: Yao Q. Y. , Ogan P. , Rowe M. , Wood M. , Rickinson A. B. 
Published: February 2007

AbstractIn this study, infectious Epstein‐Barr virus (EBV) shedding in the oropharynx and numbers of virus‐infected B cells in the blood have been monitored in long‐term virus carriers receiving acyclovir (ACV) therapy for herpes zoster. Eleven patients on oral ACV were followed prospectively before, during and for 2 weeks after treatment. As expected, the low levels of EBV shedding in these virus carriers (measured as cord‐blood lymphocyte transforming activity in throat washings) were eliminated during the period of ACV treatment and returned at later times. Over the same period, however, the frequency of virus‐infected B cells in the blood (measured by spontaneous transformation in limiting dilution assay) remained completely unchanged. Regression assays showed that these same patients had normal levels of EBV‐specific cytotoxic T‐cell immunity, so that the in vivo persistence of virus‐infected B cells could not be ascribed to a defect in T‐cell surveillance. We infer that the in vivo half‐life of the virus‐infected B‐cell pool in long‐term virus carriers is measured in months rather than days. We further suggest that such persistence requires a novel form of virus: B‐cell interaction distinct from the type of “latent” infection displayed by in vitro‐transformed cells.
Glycosphingolipids in lectin‐resistant variants of mouse lewis lung carcinoma cells
Authors: Ugorski Maciej , Påhlsson Peter , Dus Danuta , Nilsson Bo 
Published: February 2007

AbstractNeutral glycolipids and gangliosides from murine Lewis lung carcinoma cell line LL2 and its lectin‐resistant variants, differing in metastatic properties, were studied by fast‐atom‐bombardment mass spectrometry (FAB‐MS), exoglycosidase treatment and an immunostaining procedure. The neutral glycolipids identified in all cell lines studied included CMH, CDH, CTH, asialo GM2, globoside and a glycolipid with a preliminary structure of Hex‐Hexl‐4HexNAc‐Hex‐Hex‐Cer. The major gangliosides were GM3, GM2, GM1 and GD1a. No qualitative differences in glycosphingolipid expression were found between the metastatic cell lines (LL2 and LL2AAA) and the weakly metastatic variants (LL25, LL28, LL230 and LL2RCA II). Some quantitative differences were observed between the cell lines, e.g., in the level of ganglioside‐bound sialic acid, which was not apparently correlated with the metastatic capacities.
Inhibition of experimental metastasis of murine fibrosarcoma cells by oligopeptide analogues to the fibronectin cell‐binding site
Authors: Bretti Sergio , Neri Paolo , Lozzi Luisa , Rustici Mauro , Comojlio Paolo , Giancotti Filippo , Tarone Guido 
Published: February 2007

AbstractWe have analyzed the effect of the synthetic oligopeptides Gly‐Arg‐Gly‐Asp‐Ser‐Pro (GRGDSP) and Gly‐Arg‐Gly‐Glu‐Ser‐Pro (GRGESP), analogues to the fibronectin cell‐binding sequence, on the formation of experimental lung metastasis. SR‐BALB were injected alone or in conjunction with GRGDSP or GRGESP in the tail vein of BALB/c mice. Co‐injection with GRGESP reduced by approximately 70% the number of metastatic colonies per mouse. However, co‐injection with the closely related peptide GRGDSP, containing the conservative substitution Glu/Asp, did not affect metastatic behavior. GRGESP peptide anti‐metastatic activity was not due to a toxic effect on tumor cells or on mice. In vitro adhesion assays testing for a possible effect of the peptide on cell‐matrix interactions indicated that the GRGESP peptide did not affect cell adhesion to the matrix proteins tested.
Hyposialylation of high‐molecular‐weight membrane glycoproteins parallels the loss of metastatic potential in wheat‐germ agglutinin‐resistant friend leukemia cells
Authors: Benedetto A. , Elia G. , Sala A. , Belardeli F. 
Published: February 2007

AbstractFrom the highly metastatic in vivo‐passaged Friend leukemia cells (FLC), WGA‐resistant (WR) tumor cell variants were selected. These WR FLC had lost their capacity to metastasize when injected i.v. or s.c. into DBA/2 mice. We have characterized the plasma membrane glycoproteins of the different FLC types by: (i) metabolic labelling with (3H)‐galactose; (ii) surface labelling with galactose oxidase‐borohydride; (iii) direct binding of (125l)‐lectins on glycoproteins separated by SDS‐PAGE. The ensemble of these approaches showed that the 100‐ to 200‐kDa glycoproteins of in vivo‐passaged FLC and WR FLC exhibited a very similar distribution of the terminal galactose in their oligosaccharide moieties. In contrast, the expression of terminal sialic acid was reduced in WR FLC with respect to in vivo‐passaged counterparts as appreciated by: (i) binding experiments with (125l)‐WGA; (ii) cathodic shift of the 100‐ to 200‐kDa glycoproteins in 2‐dimensional electrophoresis studies, and (iii) thiobarbituric acid assay after FLC treatment with neuraminidase. Moreover, binding experiments with (125l)‐LPHA, (125l)‐ConA and (125l)‐WGA (after Smith degradation) indicated that, in the 100‐to 200‐kDa region, virtually identical asparagine‐linked tri‐ or tetra‐antennary complex‐type oligosaccharides were expressed in both cell types. We conclude that the sialylation of high‐molecular‐weight surface glycoproteins (particularly in the 150‐kDa region) is strongly associated with the metastatic potential of FLC, especially to the liver.