bluuebunny/crossref_metadata_2025_split huggingface dataset:
Слід біорізноманіття продукту: теорія і методологія розрахунку
Authors: Pelyukh Oksana , Soloviy Ihor , Kiyko Orest , Ilkiv Mykhailo , Chelepis Taras , Lavnyy Vasyl 
Published: April 2024

Досліджено теоретичні положення та сутність поняття «слід біорізноманіття», встановлено ключові аспекти методології його розрахунку. Зокрема, розглянуто визначення терміну «слід біорізноманіття» у вітчизняній та зарубіжній науковій літературі. Запропоновано визначати слід біорізноманіття як вплив товару, компанії, людини чи спільноти на глобальне біорізноманіття, виміряний у термінах зміни біологічного різноманіття в результаті виробництва та споживання певних товарів і послуг. Виокремлено та проаналізовано основні підходи та інструменти розрахунку сліду біорізноманіття. Розглянуто шість основних груп інструментів розрахунку сліду біорізноманіття і встановлено ключові відмінності між ними. Загалом розрахунки сліду біорізноманіття із використанням вищевказаних інструментів базуються на інформації компанії чи підприємства (фінансових даних або/і даних ланцюжка створення вартості), враховуючи географічний, екосистемний контексти або узагальнені (проксі) зв’язки та дотримуються трьохетапної послідовності. Доцільним є застосування сліду біорізноманіття як індикатора для різних секторів і рівнів управління, їх видів діяльності чи ланцюгів постачання, а також для окремого продукту чи споживача. Слід біорізноманіття, як індикатор, може допомогти бізнесу в обґрунтуванні стратегічних підходів, управлінських і технологічних рішень у контексті забезпечення належної екологічної та соціальної відповідальності. Розрахунок сліду біорізноманіття є важливим інструментом збереження екосистем і природних ландшафтів, забезпечення сталого використання ресурсів внаслідок прийняття екологічно відповідальних рішень.
Identification and structural characterization of potential degradation products of baricitinib using liquid chromatography combined with quadrupole time-of-flight mass spectrometry
Authors: Kakouri Eleni , Gkountanas Konstantinos , Kanakis Charalabos , Tarantilis Petros A. , Dotsikas Yannis 
Published: April 2024

AbstractBaricitinib (BRT) is a drug substance with potent anti-inflammatory activity indicated in rheumatoid arthritis, atopic dermatitis, severe alopecia areata and recently for the treatment of Covid-19. Process impurities of the drug during its formulation are quite known, however studies regarding its degradation products (DPs) under stress conditions are limited. In this study, the drug was subjected to forced degradation under various degradation conditions, including acidic hydrolysis, alkaline hydrolysis, oxidative and thermal, to determine its inherent stability. To this purpose, a novel HPLC method was developed for the determination of degradation impurities of BRT. Alkaline hydrolysis test showed a selectivity towards breaking C–C bonds. This resulted to five DPs formed by chain scission reactions occurred at the pyrrolo-pyrimidine group between C6–C10 and C8–C9. Also, the ethylsulfonyl-azetidin-ylidene group was subjected to C–C bond cleavage at C12–C15 and C16–C18. Degradation products were further characterized with the use of liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-Q-TOF-MS/MS).
The Effects of Pantoprazole on Kidney Outcomes
Authors: Pyne Lonnie , Smyth Andrew , Molnar Amber O. , Moayyedi Paul , Muehlhofer Eva , Yusuf Salim , Eikelboom John , Bosch Jacqueline , Walsh Michael 
Published: April 2024

Key Points

In this post hoc analysis of a randomized controlled trial, the proton pump inhibitor pantoprazole led to a faster rate of eGFR decline as compared with placebo.Additional studies are needed to determine the effect of proton pump inhibitors on those at higher risk of adverse kidney outcomes.

Background
Observational studies have found an association between proton pump inhibitor use and worsening kidney function. It is unclear whether these associations are causal. We conducted post hoc analyses to determine the effect of pantoprazole on kidney function using data from the Cardiovascular Outcomes for People Using Anticoagulation Strategies (COMPASS) trial, a 17,598-participant randomized trial comparing pantoprazole (8791) with placebo (8807).

Methods
The primary outcome was the rate of eGFR change. Rate of eGFR change was based on the two eGFR measures available: the eGFR at randomization and at the open-label extension study that enrolled at trial conclusion. Secondary outcomes included incident CKD (defined by eGFR <60 ml/min per 1.73 m2 at open-label extension or case report forms) as well as AKI, acute nephritis, and nephrotic syndrome.

Results
Eight thousand nine hundred ninety-one of the 17,598 participants randomized to pantoprazole/placebo (51%) had eGFR recorded at baseline and open-label extension enrollment and were included in the rate of eGFR change population (mean age 67 [SD 8] years, 22% female, mean baseline eGFR 75 [SD 17.5] ml/min per 1.73 m2). The mean duration between randomization and open-label extension eGFR was 3.3 (SD 0.8) years. The placebo rate of eGFR change was −1.41 (SD 4.45) ml/min per 1.73 m2 per year. The pantoprazole rate of eGFR change was −1.64 (SD 4.47) ml/min per 1.73 m2 per year. In adjusted analyses, pantoprazole had a 0.27 ml/min per 1.73 m2 per year greater decline in eGFR (95% confidence interval [CI], 0.11 to 0.43). The odds ratio for the effect of pantoprazole on incident CKD was 1.11 (95% CI, 0.98 to 1.25) and on AKI was 0.89 (95% CI, 0.65 to 1.21). There were five nephrotic syndrome outcomes recorded and one event of acute nephritis.

Conclusions
In this post hoc analysis of the COMPASS trial, pantoprazole resulted in a statistically significant greater rate of eGFR decline as compared with placebo.

Clinical Trial registry name and registration number:
Rivaroxaban for the Prevention of Major Cardiovascular Events in Coronary or Peripheral Artery Disease (COMPASS), NCT01776424.
Intraosseous resection of bone tumors: our experience
Authors: Teplyakov V. V. , Dobrosotsky S. V. , Shaposhnikov A. A. , Lazukin A. V. , Ukhvarkin A. P. , Saprykina E. A. , Solodky V. A. 
Published: April 2024

Introduction. The effectiveness of efficient surgical interventions in patients with benign, borderline, and malignant bone tumors remains an important topic because such operations imply lower injury rate and volume of surgical intervention while being more physiological. In terms of radicality and consequently recurrence rates they are comparable to more traumatic surgeries such as segmental resections with various types of reconstructions (arthrodesis, endoprosthesis, auto- and allotransplantation using bone fragments, et al) and amputation.Aim. To analyze the results of intraosseous resection for benign, malignant, giant cell, and metastatic bone tumors.Materials and methods. Between 2015 and 2023, 45 interventions of intraosseous resection were performed in 44 patients: 19 (43 %) had enchondroma, 6 (14 %) – giant cell tumor, 4 (9 %) – fibrous dysplasia, 3 (7 %) – solitary bone cyst, 3 (7 %) – aneurysmal bone cyst, 2 (5 %) – chondromyxoid fibroma, 4 (9 %) – metastatic bone lesions, 1 (2 %) – osteoid osteoma, 1 (2 %) – benign fibrous histiocytoma, 1 (2 %) – chondrosarcoma G1 . Among them, 27 interventions were performed in combination with adjuvants (liquid nitrogen cryoablation, argon plasma coagulation, hydrogen peroxide, ethanol). In 24 patients, reconstruction was performed using lyophilized corpse bones, in 5 using autotransplant (fragments of the iliac crest), in 10 using plastic material (in 6 – polymethacrylate, in 4 – osseointegrative material “Rekost”), in 6 using xenoplastic material “Silorif.” Additionally, 16 surgeries were augmented with osteosynthesis (14 extramedullary and 3 intramedullary osteosyntheses).Results. Complications in the early postoperative period were observed in 3 (7 %) patients, in the late postoperative period in 2 (5 %) patients. At follow ups (between 2 and 97 months), 42 (95 %) patients did not have signs of recurrence. Musculoskeletal Tumor Society Score (MSTS) at these follow ups in recurrence-free patients was excellent (mean score – 93.6 %) and good (mean score – 77 %). Eastern Cooperative Oncology Group (ECOG) performance status also showed high functional potential of intraosseous resection: its mean value was 0.3.Conclusion. Use of intraosseous resection including augmentation with adjuvant measures, allowed to achieve excellent functional results in 42 (95 %) of patients with mean follow-up duration of 39.8 months. Fractures in 2 patients demonstrate the necessity of extramedullary osteosynthesis in surgeries on long bones experiencing high load irrespective of the volume of intervention. The obtained results show applicability and high effectiveness of intraosseous resection. For certain indications, it can be the treatment of choice.
Inserción laboral de migrantes y refugiados venezolanos en Uruguay antes y durante el Covid-19
Authors: Facal Silvia 
Published: April 2024

Resumen:
Desde los últimos años del tercer mandato de Hugo Chávez la República Bolivariana de Venezuela se encuentra atravesando una profunda crisis política, económica y social, la cual ha provocado una salida masiva de población venezolana hacia el exterior recrudecida por la crisis sanitaria del Covid-19. Uruguay, con una política migratoria flexible, se ha convertido en un destino ventajoso a la hora de obtener la documentación necesaria para residir y trabajar en igualdad de condiciones con la población nacional. El acceso al mercado laboral se ha tornado en la principal meta de estos migrantes y refugiados ya que este les permite acceder a la vivienda, la salud y al sistema financiero. Su acceso presenta particularidades vinculadas a las necesidades del mercado laboral uruguayo y a la calificación presentada por los migrantes y refugiados, analizada desde una perspectiva teórica por teorías como la de los mercados duales. En este artículo presentamos los resultados obtenidos de una investigación mixta realizada, entre 2018 y 2022, sobre la experiencia migratoria laboral de los migrantes y refugiados venezolanos en Uruguay.
Teletrabalho Eficaz: avaliação do desenho instrucional em treinamentos
Authors: Carmo Eliane Almeida do , Abbad Gardênia da Silva , Miranda Vivian dos Santos , Meira Letícia Mara de Lima 
Published: April 2024

O objetivo deste trabalho é avaliar a qualidade do Desenho Instrucional (DI) de um programa de treinamentos de habilidades de trabalho em equipes virtuais oferecido por uma organização brasileira do segmento financeiro. O programa foi desenvolvido para atender à demanda de trabalho virtual emergencial na pandemia de Covid-19 e possui 73 horas para adequação ergonômica, assertividade da comunicação e desenvolvimento de empatia, dentre outros. Os dados foram obtidos por meio de fontes secundárias, com a análise dos conteúdos e do ambiente do treinamento virtual. A análise da qualidade foi realizada por 14 especialistas, com o apoio de um roteiro em três rodadas avaliativas e foi considerado o consenso entre os juízes especialistas. Os resultados mostraram que os conteúdos dos treinamentos foram voltados tanto para a equipe, quanto para o indivíduo e foram relevantes para preparar os trabalhadores a dar continuidade aos seus trabalhos a partir de casa. Entretanto, o DI dos treinamentos teve pouca proximidade com a teoria e os princípios instrucionais, o que pode comprometer o potencial para proporcionar a mudança de comportamento nos egressos e alcançar os desempenhos esperados. A partir dos resultados e da literatura de DI, foram apresentadas sugestões que podem orientar gestores e desenhistas instrucionais em futuros treinamentos.
Sala de aula invertida e história da Matemática
Authors: Moura Roseli Alves de , Brandão Kelven Cleyton de Araujo 
Published: April 2024

Objetivo: O presente trabalho objetiva estreitar o diálogo entre a matemática no ensino superior e a história da matemática, como forma de promover a construção de interfaces entre estes campos de saberes, elencando como temática o trabalho de Jean-Baptiste Joseph Fourier (1768-1830), que consagrou-se em 1822 com a publicação da obra Théorie analytique de la chaleur.. Metodologia: Uma abordagem qualitativa, de cunho biográfico e com objetivos exploratórios envolvendo discentes da disciplina Cálculo Diferencial e Integral em um curso de Engenharia em um Centro Universitário em São Paulo. Adotamos a metodologia de ensino de Sala de Aula Invertida e uma abordagem sob o viés da “história”, segundo Grattan-Guinness. Resultados: Consideramos o legado do personagem Fourier, tido como fundamental na discussão sobre a redefinição do conceito de função, ao extrapolar conceitos matemáticos que estavam enraizados na sociedade acadêmica nos oitocentos. O protagonismo dos estudantes e o tempo ganho em sala de aula para discussões acerca do conteúdo Séries de Fourier, revelou-se decorrente da metodologia adotada em permanente diálogo com a abordagem histórica. O arcabouço teórico trazido pelos estudantes – representados pela maioria das referências bibliográficas utilizadas neste artigo, traz indícios de amadurecimento, favorecendo possibilidades de desdobramentos em experimentos didáticos futuros. Conclusão: Atualmente, as inúmeras aplicações dos estudos de Fourier encontram-se presentes, explicita ou implicitamente, em diversas áreas no âmbito das engenharias, alcançando as nossas vidas de forma inexorável, transcendendo muitas vezes, a mera aplicação de tecnologia.
Provision of a Home-Based Video-Assisted Therapy Program Is Noninferior to In-Person Hand Therapy After Thumb Carpometacarpal Arthroplasty
Authors: Barrett Patrick C. , Hackley Darren T. , Yu-Shan Andrea A. , Shumate Tracy G. , Larson Kathryn G. , Deneault Christopher R. , Bravo Cesar J. , Peterman Nicholas J. , Apel Peter J. 
Published: April 2024

Background:
In-person hand therapy is commonly prescribed for rehabilitation after thumb carpometacarpal (CMC) arthroplasty but may be burdensome to patients because of the need to travel to appointments. Asynchronous, video-assisted home therapy is a method of care in which videos containing instructions and exercises are provided to the patient, without the need for in-person or telemedicine visits. The purpose of the present study was to evaluate the effectiveness of providing video-only therapy (VOT) as compared with scheduled in-person therapy (IPT) after thumb CMC arthroplasty.

Methods:
We performed a single-site, prospective, randomized controlled trial of patients undergoing primary thumb CMC arthroplasty without an implant. The study included 50 women and 8 men, with a mean age of 61 years (range, 41 to 83 years). Of these, 96.6% were White, 3.4% were Black, and 13.8% were of Hispanic ethnicity. The primary outcome measure was the Patient-Reported Outcomes Measurement Information System (PROMIS) Upper Extremity (UE) score. Subjects in the VOT group were provided with 3 videos of home exercises to perform. Subjects in the control group received standardized IPT with a hand therapist. Improvements in the PROMIS UE score from preoperatively to 12 weeks and 1 year postoperatively were compared.

Results:
Fifty-eight subjects (29 control, 29 experimental) were included in the analysis at the 12-week time point, and 54 (27 control, 27 experimental) were included in the analysis at the 1-year time point. VOT was noninferior to IPT for the PROMIS UE score at 12 weeks and 1 year postoperatively, with a difference of mean improvement (VOT − IPT) of 1.5 (95% confidence interval [CI], −3.6 to 6.6) and 2.2 (95% CI, −3.0 to 7.3), respectively, both of which were below the minimal clinically important difference (4.1). Patients in the VOT group potentially saved on average 201.3 miles in travel.

Conclusions:
VOT was noninferior to IPT for upper extremity function after thumb CMC arthroplasty. Time saved in commutes was considerable for those who did not attend IPT.

Level of Evidence:
Therapeutic Level I. See Instructions for Authors for a complete description of levels of evidence.
Impairment of FBXO31-mediated Ubiquitination of OGT Upregulates O-GlcNAcylation to Advance Endometrial Malignancy
Authors: Yuan Kai , Zhang Na , Meng Yang , Mao Song , Ni Huiling , Huang Canhua , Shen Licong , Fu Kun , Lv Lu , Yu Chunhong , Chen Fang , Zhang Yu 
Published: April 2024

Abstract

Aberrant O-GlcNAc cycling of the cancer proteome is a manifestation of its metabolic plasticity. As one of the most common cancer of the female genital tract associated with metabolic syndrome, endometrial cancer (EC) tissues often bear altered O-GlcNAcylation patterns. However, integration of O-GlcNAc status with existing histomorphologic and molecular subtypes of EC in large cohorts and identification of molecular modules controlling the O-GlcNAc homeostasis remain to be accomplished. Here we establish a positive correlation of O-GlcNAcylation with histologic grade of EC in a Chinese cohort containing 219 tumors and consolidate it in The Cancer Genome Atlas (TCGA) EC dataset. Higher O-GlcNAc level is associated with less pathological differentiation and poorer prognosis. Functionally, increasing O-GlcNAcylation promotes proliferation and stem-like cell properties in normal endometrial epithelial organoids (EE-Os), whereas decreasing O-GlcNAcylation limits the growth of endometrial cancer organoids (EC-Os). Using genome-wide CRISPR screen, we further identify that the F-box only protein 31 (FBXO31), whose loss of heterozygosity is frequently observed in cancer, regulates O-GlcNAc homeostasis. FBXO31 acts as a substrate receptor of the SCF ubiquitin ligase complex to ubiquitinate the O-GlcNAc transferase OGT. Loss of FBXO31 results in accumulation of OGT and upregulation of O-GlcNAcylation in EC. Our study highlights the O-GlcNAcylation as a useful stratification marker and potential therapeutic target for the advanced, poorly differentiated EC cases.
Musashi-2 (MSI2) regulation of DNA damage response in lung cancer
Authors: Bychkov Igor , Deneka Alexander , Topchu Iuliia , Pangeni Ragendra , Ismail Amr , Lengner Christopher , Karanicolas John , Golemis Erica , Makhov Peter , Boumber Yanis 
Published: April 2024

Abstract
Lung cancer is one of the most common types of cancer worldwide. Non-small cell lung cancer (NSCLC), typically caused by KRAS and TP53 driver mutations, represents the majority of all new lung cancer diagnoses. Overexpression of the RNA-binding protein (RBP) Musashi-2 (MSI2) has been associated with NSCLC progression. To investigate the role of MSI2 in NSCLC development, we compared the tumorigenesis in mice with lung-specific Kras-activating mutation and Trp53 deletion, with and without Msi2 deletion (KPM2 versus KP mice). KPM2 mice showed decreased lung tumorigenesis in comparison with KP mice. In addition, KPM2 lung tumors showed evidence of decreased proliferation, but increased DNA damage, marked by increased levels of phH2AX (S139) and phCHK1 (S345), but decreased total and activated ATM. Using cell lines from KP and KPM2 tumors, and human NSCLC cell lines, we found that MSI2 directly binds ATM mRNA and regulates its translation. MSI2 depletion impaired DNA damage response (DDR) signaling and sensitized human and murine NSCLC cells to treatment with PARP inhibitors in vitro and in vivo. Taken together, we conclude that MSI2 supports NSCLC tumorigenesis, in part, by supporting repair of DNA damage by controlling expression of DDR proteins. These results suggest that targeting MSI2 may be a promising strategy for lung cancers treated with DNA-damaging agents.
A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time
Authors: Chen Thomas , Way Ryan , Templeton Hayley , Ball Daniel , Cheng Ming-Hao , Tobet Stuart 
Published: April 2024

Abstract

Epithelial cells create barriers that protect many different components in the body from their external environment. The gut in particular carries bacteria and other infectious agents. A healthy gut epithelial barrier prevents unwanted substances from accessing the underlying lamina propria while maintaining the ability to digest and absorb nutrients. Increased gut barrier permeability, better known as leaky gut, has been linked to several chronic inflammatory diseases. Yet understanding the cause of leaky gut and developing effective interventions are still elusive due to the lack of tools to maintain tissue’s physiological environment while elucidating cellular functions under various stimuli ex vivo. This paper presents a microphysiological system capable of recording real-time barrier permeability of mouse gut tissues in a realistic physiological environment over extended durations. Key components of the microphysiological system include a microfluidic chamber designed to hold the live tissue explant and create a sufficient microphysiological environment to maintain tissue viability; proper media composition that preserves a microbiome and creates necessary oxygen gradients across the barrier; integrated sensor electrodes and supporting electronics for acquiring and calculating transepithelial electrical resistance (TEER); and a scalable system architecture to allow multiple chambers running in parallel for increased throughput. The experimental results demonstrate that the system can maintain tissue viability for up to 72 hours. The results also show that the custom-built and integrated TEER sensors are sufficiently sensitive to distinguish differing levels of barrier permeability when treated with collagenase and low pH media compared to control. Permeability variations in tissue explants from different positions in the intestinal tract were also investigated using TEER revealing their disparities in permeability. Finally, the results also quantitatively determine the effect of the muscle layer on total epithelial resistance.
Plasma Circulating Tumor DNA Sequencing Reveals the Landscape of Acquired Mutations in Patients with Hepatocellular Carcinoma: a Potential Predictive Value in Liquid Biopsy
Authors: Wu Xiaolin , Borchmann Sven , Heger Jan-Michel , Kasper Philipp , Li Jiahui , Bamberger Margaux , Gassa Asmae , Alakus Hakan , Odenthal Margarete , Drebber Uta , Altmüller Janine , Becker Kerstin , Stippel Dirk , Bruns Christiane J , Zhao Yue , Wahba Roger 
Published: April 2024

Abstract
Background
Recent advances in circulating tumor DNA (ctDNA) analysis offer a promising approach for diagnosing and monitoring hepatocellular carcinoma (HCC). This study focused on the potential clinical role of ctDNA analysis in HCC management.
Materials and methods:
Thirty patients with HCC and 10 with non-malignant liver disease were enrolled in this study. Circulating free nucleic acids, germline DNA, and tumor DNA (tDNA) from both blood samples and paraffin-embedded tumor biopsies were analyzed by a panel targeting 100 common HCC-related genes.
Results
The ctDNA mutations were identified in 66.6% of HCC patients. New ctDNA mutations were identified, among them NCOR2 having the highest frequency (13%), the same with classical mutation CTNNB1. Gene sets composed of several mutations in ctDNA have the potential to predict the prognosis of HCC. A higher proportion of concordant mutations was also detected in HCC patients with tumor vascular invasion (p=0.045). Combining the ctDNA mutations and the Alpha-fetoprotein (AFP) level revealed more diagnostic accuracy than either the mutations or AFP alone, with p-values of 0.028 and 0.009, respectively.
Conclusion
Liquid biopsy-based analysis of ctDNA mutations may offer considerable benefits to diagnostic systems for HCC.