bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lymphoglandular bodies in lymphoid lesions and non‐lymphoid round cell tumors: A quantitative assessment
Authors: Francis Issam M. , Das Dilip K. , Ai‐Rubah Nawal A. R. , Gupta Subhash K. 
Published: February 2007

AbstractLymphoglandular bodies (cytoplasmic fragments) were counted in 10 high power fields (HPF) of the smears in 35 cases of non‐Hodgkin lymphoma (NHL), 26 Hodgkin's disease (HD), 36 reactive lymphadenitis, 24 non‐lymphoid round cell tumors, and 20 cases of secondaries in lymph nodes. The number of lymphoglandular bodies in both May‐Grünwald‐Giemsa (MGG) and hematox‐ylin‐eosin (H&E) stained smears in NHL, HD, and reactive lymphadenitis were significantly higher compared to non‐lymphoid round cell tumors (P < 0.001). In H&E stained smear 92.5% of NHL, 86.2% of reactive lymph nodes, and 66.7% of HD cases had > 100 lymphoglandular bodies per 10 HPF. In MGG stained smears 100% NHL, 89.7% of reactive lymphadenitis, and 66.7% of HD cases contained > 100 lymphoglandular bodies per 10 HPF. On the other hand only 4.8% of non‐lymphoid round cell tumors crossed this level in both H&E stained and MGG stained smears. Number of lymphoglandular bodies was also significantly higher in MGG stained smears compared to H&E stained smears (P < 0.05 to < 0.01) and in fine‐needle aspiration (FNA) smears compared to imprint smears (P < 0.01 to < 0.001) in cases of NHL, HD, and reactive lymphadenitis. Diagn Cytopathol 1994; 11:23–27. © 1994 Wiley‐Liss, Inc.
HPV typing of cervical squamous lesions by in situ HPV DNA hybridization: Influence of HPV type and therapy on the follow‐up of low‐grade squamous cervical disease
Authors: Raisi Orville , Ghirardini Carla , Aloisi Piero , Cermelli Claudio , Portolani Marinella 
Published: February 2007

AbstractPapanicolaou (Pap)‐stained cervical specimens from 160 squamous lesions were processed for the detection of human papillomavirus (HPV) DNA by an in situ hybridization (ISH) assay. Three biotinylated HPV DNA probes were employed, each containing HPV genotypes 6/11, HPV genotypes 16/18, or HPV genotypes 31/35/51. The HPV etiology of 86 lesions was ascertained (53.8%). In 74 out of 135 (58.8%) HPV‐typed low‐grade squamous intraepithelial lesions (SILs), HPV 6/11 was found in nine (6.6%), HPV 16/18 in 46 (34.2%), and HPV31/35/51 in 19 lesions (14.1%); in 11 out of 18 HPV‐typed high‐grade SILs (61.1%), seven lesions (38.9%) were typed for HPV 16/18 and four (22.2%) for HPV 31/35/51. Of seven invasive carcinomas, only one (14.3%) reacted with the HPV 16/18 DNA probe. A cohort of 124 low‐grade SILs was followed cytologically for a year. The results of this study are discussed in light of HPV type association and therapy. Diagn Cytopathol 1994; 11:28–32. © 1994 Wiley‐Liss, Inc.
Matrix crystals in cytologic urine specimens: Observations on their mineral composition by energy dispersive X‐ray microanalysis and morphologic scanning electron microscopy
Authors: Zaharopoulos Paul , Wen Julie W. , Wong Jick 
Published: February 2007

AbstractCrystals consisting by light microscopy of organic matrix (matrix crystals) encountered in cytologic urine specimens of 8 patients were examined for mineral phase components by scanning electron microscopy with energy dispersive X‐ray microanalysis (SEM‐EDX) and by morphologic scanning electron microscopy (SEM) performed separately in four of the eight cases. Whenever possible (three cases) mineralized crystals present in these specimens were examined separately by SEM‐EDX for comparison of mineral phase composition with that of the corresponding matrix forms. Although by SEM‐EDX components of matrix, glass and slide preparation media interfere with the precise estimation of the mineral phase components, the results of this method supported by the SEM morphology suggest that crystals consisting of organic matrix include a mineral phase, the lattice structure of which provides them from the early stages of formation with the characteristic morphology of the fully mineralized forms. This also suggests that organic matrix plays a role in the nucleation of minerals during the formation of certain urinary crystals. Diagn Cytopathol 1994; 11:38–46. © 1994 Wiley‐Liss, Inc.
Toxoplasma pneumonia: Cytologic diagnosis by bronchoalveolar lavage
Authors: Wheeler Rebecca R. , Bardales Ricardo H. , North Paula E. , Vesole David H. , Tricot Guido , Stanley Michael W. 
Published: February 2007

AbstractTwo cases of toxoplasma pneumonia in immunocompromised hosts are reported. The diagnoses were made by the identification of trophozoites in bronchoaheolar lavage material. The organisms were more readily identified in the air‐dried Diff‐Quik‐stained smears. Cytologic findings and correlation with postmortem examination material in one case are described. A high index of suspicion for this pathogen, as well as screening cytologic preparations at medium to high magnification may increase the diagnostic rate for this uncommon but treatable disease. Diagn Cytopathol 1994; 11:52–55. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration of anaplastic thyroid carcinoma with varied cytologic and histologic patterns: A case report
Authors: Brooke P. K. , Hameed Meera , Zakowski Maureen F. 
Published: February 2007

AbstractAnaplastic carcinoma of the thyroid (ACT) is a rare subtype of thyroid neoplasm. This tumor represents approximately 5–10% of all thyroid malignancies and its poor prognosis has not changed in 25 years. ACT often arises in a long‐standing thyroid nodule and has been documented to be associated with a variety of more well‐differentiated thyroid carcinomas. We describe the cytologic features of a case in which the patient had a 10‐yr history of a thyroid nodule. The ACT in this case had a cytologic and histologic appearance of several different subtypes of thyroid carcinomas. Diagn Cytopathol 1994;11:60–63. © 1994 Wiley‐Liss, Inc.
Metastatic epithelioid hemangioendothelioma in a pleural effusion: Diagnosis by cytology
Authors: Abati Andrea , Cajigas Agustin , Hijazi Yasmine M. 
Published: February 2007

AbstractEpithelioid hemangioendothelioma (EH) is a vascular neoplasm of uncertain malignant potential. We report a 22‐yr‐old female with a primary malignant EH of the iliac bone with adjacent soft tissue involvement which, during its metastatic course, presented as a pleural effusion. The effusion was cellular with tumor cells present both singly and in clusters. Distinguishing cytologic features included cytoplasmic vacuolization consistent with primitive intracytoplasmic lumen formation, variability in cell size, biphasic cytoplasmic staining with Diff‐Quik stain, multinucleation, cell in cell engulfment, and multiple prominent nucleoli. Differential diagnosis based on morphology included malignant mesothelioma and adenocarcinoma. Immunocytochemical stains on the neoplastic cells were positive for Ulex Europaeus, Factor VIII‐related antigen, and CD34, reflecting vascular differentiation and confirming the diagnosis of metastatic EH to the pleural cavity. Diagn Cytopathol 1994;11:64–67. © 1994 Wiley‐Liss, Inc.This article is US government work and, as such, is in the public domain in the United States of America
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p53 immunolocalization in cytology specimens: A study in human esophageal carcinoma
Authors: Goukon Yuji , Sasano Hironobu , Nishihira Tetsuro , Mori Shozo , Nagura Hiroshi 
Published: February 2007

AbstractImmunohistochemical analysis of p53 has become an established method of detecting mutated p53. Immunolocalization of p53 has been extensively studied in tissue sections of many human neoplasms, but not in cytology specimens. Therefore, we performed Immunolocalization of p53 in both cytology and tissue preparations obtained from the same specimens in 19 cases of human esophageal squamous‐cell carcinoma. Three different antibodies, PAb1801, DO‐7, and CM‐1, were employed. Positive p53 nuclear immunoreactivity was observed in carcinoma cells, but not in noncarcinomatous cells, in both tissue and cytology preparations. The incidence of p53 positivity was 53% (10/19) in cytology and 42% (8/19) in tissue, and the coincident rate of both results was 89% (17/19). We conclude that immunohistochemistry of p53 in cytology is a useful method for detecting p53 mutations, and may contribute to the diagnosis of malignancy, because it is a simple, easy, and rapid technique. Diagn Cytopathol 1994; 11:113–118. © 1994 Wiley‐Liss, Inc.
Simplified nuclear grading of fine‐needle aspirates of breast carcinoma: Concordance with corresponding histologic nuclear grading and flow cytometric data
Authors: Cajulis Ricardo S. , Hessel R. Glenn , Hwang Shyn , Haines Kenneth , Frias‐Hidvegi Denise , O'Gorman Maurice 
Published: February 2007

AbstractAlthough histologic grading of breast carcinoma is widely practiced by most pathologists, cytologic grading of fine‐needle aspirates (FNA) of this neoplasm is not commonly done. This study addresses the issue of the accuracy of a new classification system, a simplified Nuclear Grading (NG) system based on the criteria proposed by Black et al. (Surg Gynecol Obstet 1955; 100:543) in FNA of breast carcinoma. We reviewed 100 cases of breast carcinoma, initially diagnosed by fine‐needle aspiration biopsy (FNAB) with subsequent histologic confirmation, consisting of 94 ductal, five lobular, and one medullary carcinoma. NG of Papanicolaou's stained materials were reviewed twice independently by two pathologists and then were compared to the original histologic NG. The concordance rate with histology ranged from 80–90%. Intraobserver reproducibility was 86 and 88%, while interobserver reproducibility ranged from 84–88%. Of the 88 cases with corresponding flow cytometic (FCM) data, there were 35 diploid and 53 aneuploid cases. Fifty‐nine (95%) of histologic high NG were aneuploid or diploid with high S‐phase fraction (SPF), while 20 (77%) of histologic low NG were diploid with low SPF. This study confirms that nuclear grading of FNA of breast carcinoma using a simplified NG system has a high concordance with histology, has high intraobserver and interobserver reproducibility, and that this grading system correlates well with FCM analysis when tumors are simply divided based on NG as high or low grade. Diagn Cytopathol 1994; 11:124–130. © 1994 Wiley‐Liss, Inc.
Hashimoto's thyroiditis: Fine‐needle aspirations of 50 asymptomatic cases
Authors: Poropatich Cary , Marcus Devra , Oertel Yolanda C. 
Published: February 2007

AbstractThe cytologic findings from 50 cases of asymptomatic Hashimoto's thyroiditis diagnosed by fine‐needle aspiration are reviewed. All patients were referred for fine‐needle aspiration by the same physician: 48 because of thyroid enlargement and the clinical suspicion of Hashimoto's thyroiditis, two because of a small thyroid nodule. Positive antimicrosomal (antithyroid peroxidase) and/or antithyroglobulin antibody tilers (performed by the same laboratory using the same technique) were present in only 25 of 48 patients evaluated. Cytologic findings included a predominantly lymphocytic process in 92% of the cases and a predominantly oxyphilic (Askanazy/Hürthle) cell process in 8% of the cases. It is likely that all patients were in an early stage of the autoimmune process. Our data indicate that the incidence of Hashimoto's thyroiditis is considerably higher than when recognized only by serologic tests for antibodies. Diagn Cytopathol 1994; 11:141–145. © 1994 Wiley‐Liss, Inc.
Aspiration biopsy cytology of tubular carcinoma of the breast
Authors: Tos Angelo P. Dei , Giustina Dino Della , De Martin Vallì , Libera Duilio Della , Bittesini Lucia 
Published: February 2007

AbstractTubular carcinoma represents a rare, well‐differentiated type of breast malignancy, characterized by relatively unaggressive clinical behavior. Cytologic diagnosis of this subgroup of tumors is considered difficult as cell population usually presents in tightly cohesive clusters and shows minimal atypia. Cytomorphologic features of 15 examples of histologically proven pure tubular carcinomas of the breast are herein reported. If a constellation of morphologic criteria is critically evaluated, tubular carcinoma may be diagnosed on cytologic specimens.Aspiration biopsy cytology represents an optimal diagnostic tool in the preoperative diagnosis of breast masses. Its application to the detection of this special type of breast carcinoma permits better planning of therapeutic procedures. Diagn Cytopathol 1994; 11:146–150. © 1994 Wiley‐Liss, Inc.
Pulmonary carcinoid with papillary structure: Report of a case with fine‐needle aspiration cytology
Authors: Evans Hilary , Blaney Ronald 
Published: February 2007

AbstractFine‐needle aspiration biopsy of a peripheral lung mass in a 44‐yr‐old man revealed a papillary tumor. Histologic examination of the mass, removed by lobectomy, demonstrated a pulmonary carcinoid tumor with papillary structure. Most papillary tumors of the lung are malignant. Pulmonary carcinoid and pulmonary sclerosing hemangioma—benign tumors—have papillary variants. Both have been described in fine‐needle aspiration specimens and should be included in the differential diagnosis of papillary lung tumors. The cytologic features of pulmonary carcinoid are described. Diagn Cytopathol 1994;11:178–181. © 1994 Wiley‐Liss, Inc.
Cytologic patterns of metastatic thymoma: Diagnosis by fine‐needle aspiration biopsy
Authors: Reddy Vijaya B. , Reyes Cesar , Wang Helena , Walloch Jami , Doria Manuel , Castelli Melanie , Gattuso Paolo 
Published: February 2007

AbstractThymomas are the most frequent primary tumors of the anterior mediastinum. These lesions are slow growing and can be locally invasive, but extrathoracic metastases are rare, occurring in less than 2% of cases. Fine‐needle aspiration biopsy (FNAB) may be helpful in making the diagnosis of metastatic thymoma, with or without a clinical history of primary mediastinal thymoma. We report three cases of metastatic thymoma diagnosed by FNAB. Each case illustrates a distinctive cytologic pattern. While two of the patients had a history of histologically confirmed thymoma 11 and 13 years previously, a third patient presented with an enlarged supraclavicular lymph node and pulmonary nodules, and no prior diagnosis of thymoma. These cases demonstrate that based on distinctive cytologic patterns and features, a diagnosis of metastatic thymoma can be made with FNAB. Ancillary studies will often confirm the diagnosis. Diagn Cytopathol 1994;11:182–187. © 1994 Wiley‐Liss, Inc.
Quality assurance programs to meet CLIA requirements
Authors: Inhorn Stanley L. , Shalkham John E. , Mueller Gail B. 
Published: February 2007

AbstractCLIA '88 delineates specific quality assurance (QA) practices for cytology laboratories in the United States. In addition, there are general requirements that all types of clinical laboratories must meet to satisfy the CLIA law and regulations. This paper describes the origins and objectives of CLIA and it outlines the major sections of the legislation. To satisfy the regulations, every laboratory must develop its own quality assurance plan that addresses each of the specific conditions and standards. Furthermore, the laboratory must monitor its performance and document that, indeed, the QA systems are operating effectively. The article then describes the quality assurance plan of the Cytology Department of the Wisconsin State Laboratory of Hygiene. The department uses 28 monitors to determine compliance with the criteria and standards and to prevent problems in its day‐to‐day operation. When standards are not met or are exceeded, action steps are specified. Diagn Cytopathol 1994;11:195–200. © 1994 Wiley‐Liss, Inc.
Postmenopausal squamous‐cell atypias: A diagnostic challenge
Authors: Saminathan Thamarai , Lahoti Chitra , Kannan Vaidehi , Kline Tilde S. 
Published: February 2007

AbstractExfoliative cytologic studies directed toward atypical squamous cells of undetermined significance (ASCUS) and low‐grade squamous intraepithelial lesions (LSIL) have been neglected in the rising postmenopausal population. This retrospective study from 60 cases, initially interpreted as ASCUS or LSIL, concentrates on these lesions and their associated diagnostic pitfalls.Cytologic revaluation of the 60 patients revealed squamouscell abnormalities in 45 (18 ASCUS and 27 LSIL) and cervicitis alone in 15 cases. Follow‐up studies of the 18 ASCUS cases showed cervicitis in seven, persistent ASCUS in five, CIN I in five, and VAIN I‐II in one. Follow‐up of the 27 cases categorized as LSIL, showed ASCUS in two, CIN in 20 and VAIN in one, while four had cervicitis alone. Interestingly, two of these 45 patients had only vaginal dysplasia. By application of uniform diagnostic criteria, 15 of the 60 cases were reviewed as benign. Pitfalls included inflammatory and drying alterations, reactive metaplasia, and sampling problems. Thus, squamous‐cell abnormalities in postmenopausal women can be significant when artifactual alterations are eliminated. Diagn Cytopathol 1994; 11:226–230. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of childhood rhabdomyosarcoma: Reevaluation of the cytologic criteria for diagnosis
Authors: De Almeida Margarida , Stastny Janet F. , Wakely Paul E. , Frable William J. 
Published: February 2007

AbstractThe distinction between rhabdomyosarcoma (RMS) and the other small round blue cell tumors of childhood has therapeutic implications which stress the importance of a correct diagnosis. In attempts to reevaluate the cytologic criteria of this entity, we reviewed a series of 17 fine‐needle aspiration biopsies and three touch imprints from soft tissue masses. All cases had been histologically confirmed except for two cases that only had immunocytochemical and ultrastructural confirmation. The features occurring most commonly include a uniform population of tumor cells arranged as single cells and cohesive aggregates. The cells were predominantly round/polygonal, with uniform nuclei and scant to moderate amounts of cytoplasm. The nuclear chromatin was most often finely granular and hyperchromatic, while nucleoli were inconspicuous. Binucleated and multinucleated cells were found in 17 of the 20 smears. Intracytoplasmic vacuoles were present in 17 cases, ranging from occasional in one case, a few in 10 cases, and very numerous in six cases. We conclude that the presence of bi/multinucleated cells is an important clue for the diagnosis of RMS on fine‐needle aspiration biopsy. Diagn Cytopathol 1994;11:231–236. © 1994 Wiley‐Liss, Inc.
Application of impression cytology to the diagnosis of conjunctival neoplasms
Authors: Nolan Glenda R. , Hirst Lawrence W. , Wright R. Gordon , Bancroft B. Josephine 
Published: February 2007

AbstractConjunctival impression cytology using cellulose acetate filter paper was employed to obtain multiple samples of conjunctival epithelium from 192 normal and abnormal eyes. Modifications of an established technique were used and the impressions were stained by the Papanicolaou method and mounted on glass slides. The impressions were examined for their effectiveness in demonstrating the morphological characteristics of normal epithelium and the features of the more common conjunctival neoplasms. The technique was found to be successful at both removing cells and preserving cytologic features. It also had several advantages over other collection techniques. Overlapping cells were not a detrimental feature of the preparations from this study. Diagn Cytopathol 1994;11:246–249. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy diagnosis of extrarenal malignant rhabdoid tumor
Authors: Akhtar Mohammed , Kfoury Hala , Haider Abdulrazzaq , Sackey Kwesi , Ali M. Ashraf 
Published: February 2007

AbstractTwo cases of extrarenal malignant rhabdoid tumors are presented in which diagnosis was suggested by fine‐needle aspiration biopsy and confirmed by histologic and electron microscopic examination. Fine‐needle aspiration smears in both cases revealed round to polygonal cells with vesicular nuclei and prominent nucleoli. Several tumor cells contained cytoplasmic inclusions composed of intermediate filaments. A majority of the tumor cells stained strongly for vimentin and cytokeratin. Electron microscopic examination revealed many cells with large aggregates of intermediate filaments corresponding to the cytoplasmic inclusions. Fine‐needle aspiration biopsy may be used for diagnosing malignant rhabdoid tumor. The diagnosis may be further confirmed by immunohistochemistry and electron microscopy. Diagn Cytopathol 1994;11:271–276. © 1994 Wiley‐Liss, Inc.
Pitfalls in the cytologic diagnosis of angiosarcoma of the breast by fine‐needle aspiration: A case report
Authors: Carson Karen F. , Hirschowitz Sharon L. , Nieberg Roberta K. , Sadeghi Shala 
Published: February 2007

AbstractThis paper describes a case of angiosarcoma of the breast in a 26‐yr‐old female. The tumor, originally thought to be granulation tissue on fine‐needle aspiration biopsy, was correctly identified as a malignant neoplasm of probable mesenchymal origin on a repeat FNA biopsy 4 mo later. A diagnosis of angiosarcoma was made on a subsequent excisional biopsy. Review of the cytologic features revealed findings that should suggest angiosarcoma, especially when correlated with the clinical history. The authors describe the cytological features seen in this case, and discuss an important pitfall in the diagnosis of angiosarcoma of the breast on FNA. Diagn Cytopathol 1994; 11:297–300. © 1994 Wiley‐Liss, Inc.
Retrospective analysis of non‐correlating cervical smears and colposcopically directed biopsies
Authors: Sidawy Mary K. , Siriaunkgul Sumalee , Frost Andra R. 
Published: February 2007

AbstractThe purpose of this study is to evaluate the cause of discrepancies between non‐correlating cytologic and histologic cervical samples. The biopsy results of 433 women examined colposcopically were compared to their referral cervical smears (RS). There was a discrepancy between the RS and the subsequent biopsy in 120 women (28%). One hundred of these 120 RS were available for review; and in each case, a reason for the discrepancy was established and classified as RS overcall, RS undercall, RS sampling error, or biopsy sampling error. Fifty‐one discrepant RS were overcalled. They were reported initially as condyloma (19), mild dysplasia (22), and moderate dysplasia (10). One RS was undercalled. Nine RS were not diagnostic of the biopsy‐proven lesion due to smear sampling error. The discrepancies in the remaining 39 cases were due to biopsy sampling error. Twenty‐one of these 39 cases had additional biopsies or smears that confirmed the presence of condyloma/dysplasia, and 18 had negative follow‐up. In summary, discrepancies were a result of pathologists' interpretative error, predominantly overcalls, in 52% of non‐correlating cases, and smear or biopsy sampling error in the remaining 48%. Diagn Cytopathol 1994;11:343–347. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of vertebral body lesions: Cytologic, pathologic, and clinical correlations of 57 cases
Authors: Carson Henry J. , Castelli Melanie J. , Reyes Cesar V. , Gattuso Paolo 
Published: February 2007

AbstractWe reviewed 57 cases of fine‐needle aspiration biopsy (FNAB) specimens of vertebral body lesions at our institutions and sought correlations between clinical histories, aspirates, and tissue specimens. Patients'ages ranged from 10–86 yr (mean 62 yr). Previous clinical histories included malignancy (n = 33), osteomyelitis or systemic infection (n = 18), and nonspecific (n = 6). FNAB diagnoses were rendered in 81% of cases (n = 46) and were correlated with previous clinical history (P < 0.004). Tissue diagnoses were also correlated with previous clinical history (P < 0.02). In 19% of cases (n = 11), FNAB was unsatisfactory; of these, tissue specimens were diagnostic for five cases. In 19% of cases (n = 11), both aspirates and tissue specimens were available, and the diagnoses coincided in all cases; tissue specimens did not enhance the results of aspirates which produced positive results. Aspirates which showed normal bone marrow elements appeared to effectively rule‐out metastatic malignancy based on clinical follow‐up (mean 3.7 yr). Diagn Cytopathol 1994;11:348–351. © 1994 Wiley‐Liss, Inc.