bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence of zoonotic pathogens through biophysically induced genomic variance
Authors: Alsufyani Daniah 
Published: March 2024

Abstract
Zoonoses are infectious agents that are transmissible between animals and humans. Up to 60% of known infectious diseases and 75% of emergent diseases are zoonotic. Genomic variation between homeostatic populations provides a novel window into the effect of environmental pathogens on allelic distributions within the populations. Genodynamics is a biophysical approach utilizing developed metrics on biallelic single-nucleotide polymorphisms (SNPs) that can be used to quantify the adaptive influences due to pathogens. A genomic free energy that is minimized when overall population health is optimized describes the influence of environmental agents upon genomic variation. A double-blind exploration of over 100 thousand SNPs searching for smooth functional dependencies upon four zoonotic pathogens carried by four possible hosts amidst populations that live in their ancestral environments has been conducted. Exemplars that infectious agents can have significant adaptive influence on human populations are presented. One discussed SNP is likely associated with both adaptive and innate immune regulation. The adaptive response of another SNP suggests an intriguing connection between zoonoses and human cancers. The adaptive forces of the presented pathogens upon the human genome have been quantified.
High‐Resolution Intrashell Oxygen Isotope Studies of Cathaica fasciola and Bradybaena ravida Land Snails and Their Environmental Implications
Authors: Li Qianya , Dong Jibao , Yan Hong , Huang Huayu , Zong Xiulan , Wang Guozhen , Liu Chengcheng , Cao Yunning , Liu Weiguo , An Zhisheng 
Published: March 2024

AbstractIntrashell oxygen isotope (δ18Os) analyses of terrestrial snails have been carried out over two decades. However, the intraspecies/interspecies differences are not yet well understood. Here, we conducted a high‐resolution intrashell δ18Os study on 43 shells from Cathaica fasciola and Bradybaena ravida, and 1449 δ18Os data were obtained. These large amounts of data demonstrate the reproducibility of intrashell δ18Os. Our results suggest that C. fasciola and B. ravida have similar growing seasons mainly from March to October in the studied region. The ecological habits of the two species could explain the distinctive variations in their δ18Os sequences. Therefore, our study may interpret the discrepancy between the studies of land snail species and the stable/clumped isotopes of their shells. Moreover, the calculated average growth rate is ∼290 μm/day (from 250 to 330 μm/day) for nonadult C. fasciola. Thus, the use of snail shells for reconstructing high‐resolution terrestrial climate changes is promising.
Ambient Noise Interferometry Using Ocean Bottom Seismometer Data From Active Source Experiments Conducted in the Southernmost Mariana Trench
Authors: Zhang Yayun , Xu Min , Xiao Zhuo , Zhou Yong , Yu Chuanhai , Lin Jian , Yang Hongfeng , Qiu Xuelin 
Published: March 2024

AbstractOcean bottom seismometers (OBSs) have been used to detect submarine structural and tectonic information for decades. According to signal source controllability, OBS data have generally been classified into active and passive source data categories. The former mainly focuses on the compressional wave (P‐wave) velocity inversion and always lacks valid information about the shear wave (S‐wave) velocity structure. While the latter provides structural information with limited resolution due to the aperture of the stations. Overcoming the barriers between processing these two data types will allow the reuse of a vast amount of data from active source experiments to explore the submarine S‐wave velocity structural properties. Here, we creatively applied ambient noise interferometry to invert the S‐wave velocity structure using data from active source OBS deployment conducted in the southernmost Mariana subduction zone, which had already been utilized to detect submarine P‐wave velocity structure. Considering the short time duration and relatively low quality of this type of data, a combined method of short‐segment cross‐correlation and selected time‐frequency domain phase‐weighted stacking was adopted to obtain stable cross‐correlation functions, which were subsequently used to invert S‐wave velocity structures. Compared to previous studies using different methods, our result sheds new light on the crust and upper mantle structure of the southernmost Mariana subduction zone. This method could be used to detect more information based on the reutilization of existing active source OBS data.
Glucose-6-phosphate dehydrogenase deficiency and neonatal indirect hyperbilirubinemia: a retrospective cohort study among 40,305 consecutively born babies
Authors: Al-Bedaywi Rajai Rofail Raja , Salameh Khalil Mohd Khalil , Abedin Sarfrazul , Viswanathan Brijroy , Khedr Abedalkhalik Ahmad , Habboub Lina Hussain M. 
Published: March 2024

Abstract
Background and objective
Glucose-6-phosphate dehydrogenase deficiency (G6PDD) being highly prevalent in the Middle East, the primary objective was to estimate the incidence of neonatal jaundice among G6PD-deficient neonates and to explore its association with various risk factors.

Methods
This retrospective cohort study includes 7 years data of neonates diagnosed with G6PDD between 1st January 2015, and 30 September 2022, from Al Wakra Hospital, HMC Qatar.

Results
Among the 40,305 total births, 1013 had G6PDD with an incidence of 2.51%. Of all the G6PDD babies, 24.6% (249/1013) received phototherapy and three babies required exchange transfusion. Statistically significant associations were noted between the need for phototherapy and gestational age, gestational age groups, birth weight, and birth weight groups, but logistic regression analysis showed significant association for phototherapy only with the gestational age group.

Conclusion
Universal screening and proper follow-up is essential for G6PDD as it plays crucial role in neonatal jaundice.
Roll-to-roll, high-resolution 3D printing of shape-specific particles
Authors: Kronenfeld Jason M. , Rother Lukas , Saccone Max A. , Dulay Maria T. , DeSimone Joseph M. 
Published: March 2024

AbstractParticle fabrication has attracted recent attention owing to its diverse applications in bioengineering1,2, drug and vaccine delivery3–5, microfluidics6,7, granular systems8,9, self-assembly5,10,11, microelectronics12,13 and abrasives14. Herein we introduce a scalable, high-resolution, 3D printing technique for the fabrication of shape-specific particles based on roll-to-roll continuous liquid interface production (r2rCLIP). We demonstrate r2rCLIP using single-digit, micron-resolution optics in combination with a continuous roll of film (in lieu of a static platform), enabling the rapidly permutable fabrication and harvesting of shape-specific particles from a variety of materials and with complex geometries, including geometries not possible to achieve with advanced mould-based techniques. We demonstrate r2rCLIP production of mouldable and non-mouldable shapes with voxel sizes as small as 2.0 × 2.0 µm2 in the print plane and 1.1 ± 0.3 µm unsupported thickness, at speeds of up to 1,000,000 particles per day. Such microscopic particles with permutable, intricate designs enable direct integration within biomedical, analytical and advanced materials applications.
Evolutionary trajectories of small cell lung cancer under therapy
Authors: George Julie , Maas Lukas , Abedpour Nima , Cartolano Maria , Kaiser Laura , Fischer Rieke N. , Scheel Andreas H. , Weber Jan-Philipp , Hellmich Martin , Bosco Graziella , Volz Caroline , Mueller Christian , Dahmen Ilona , John Felix , Alves Cleidson Padua , Werr Lisa , Panse Jens Peter , Kirschner Martin , Engel-Riedel Walburga , Jürgens Jessica , Stoelben Erich , Brockmann Michael , Grau Stefan , Sebastian Martin , Stratmann Jan A. , Kern Jens , Hummel Horst-Dieter , Hegedüs Balazs , Schuler Martin , Plönes Till , Aigner Clemens , Elter Thomas , Toepelt Karin , Ko Yon-Dschun , Kurz Sylke , Grohé Christian , Serke Monika , Höpker Katja , Hagmeyer Lars , Doerr Fabian , Hekmath Khosro , Strapatsas Judith , Kambartel Karl-Otto , Chakupurakal Geothy , Busch Annette , Bauernfeind Franz-Georg , Griesinger Frank , Luers Anne , Dirks Wiebke , Wiewrodt Rainer , Luecke Andrea , Rodermann Ernst , Diel Andreas , Hagen Volker , Severin Kai , Ullrich Roland T. , Reinhardt Hans Christian , Quaas Alexander , Bogus Magdalena , Courts Cornelius , Nürnberg Peter , Becker Kerstin , Achter Viktor , Büttner Reinhard , Wolf Jürgen , Peifer Martin , Thomas Roman K. 
Published: March 2024

AbstractThe evolutionary processes that underlie the marked sensitivity of small cell lung cancer (SCLC) to chemotherapy and rapid relapse are unknown1–3. Here we determined tumour phylogenies at diagnosis and throughout chemotherapy and immunotherapy by multiregion sequencing of 160 tumours from 65 patients. Treatment-naive SCLC exhibited clonal homogeneity at distinct tumour sites, whereas first-line platinum-based chemotherapy led to a burst in genomic intratumour heterogeneity and spatial clonal diversity. We observed branched evolution and a shift to ancestral clones underlying tumour relapse. Effective radio- or immunotherapy induced a re-expansion of founder clones with acquired genomic damage from first-line chemotherapy. Whereas TP53 and RB1 alterations were exclusively part of the common ancestor, MYC family amplifications were frequently not constituents of the founder clone. At relapse, emerging subclonal mutations affected key genes associated with SCLC biology, and tumours harbouring clonal CREBBP/EP300 alterations underwent genome duplications. Gene-damaging TP53 alterations and co-alterations of TP53 missense mutations with TP73, CREBBP/EP300 or FMN2 were significantly associated with shorter disease relapse following chemotherapy. In summary, we uncover key processes of the genomic evolution of SCLC under therapy, identify the common ancestor as the source of clonal diversity at relapse and show central genomic patterns associated with sensitivity and resistance to chemotherapy.
Anoxygenic phototroph of the Chloroflexota uses a type I reaction centre
Authors: Tsuji J. M. , Shaw N. A. , Nagashima S. , Venkiteswaran J. J. , Schiff S. L. , Watanabe T. , Fukui M. , Hanada S. , Tank M. , Neufeld J. D. 
Published: March 2024

AbstractScientific exploration of phototrophic bacteria over nearly 200 years has revealed large phylogenetic gaps between known phototrophic groups that limit understanding of how phototrophy evolved and diversified1,2. Here, through Boreal Shield lake water incubations, we cultivated an anoxygenic phototrophic bacterium from a previously unknown order within the Chloroflexota phylum that represents a highly novel transition form in the evolution of photosynthesis. Unlike all other known phototrophs, this bacterium uses a type I reaction centre (RCI) for light energy conversion yet belongs to the same bacterial phylum as organisms that use a type II reaction centre (RCII) for phototrophy. Using physiological, phylogenomic and environmental metatranscriptomic data, we demonstrate active RCI-utilizing metabolism by the strain alongside usage of chlorosomes3 and bacteriochlorophylls4 related to those of RCII-utilizing Chloroflexota members. Despite using different reaction centres, our phylogenomic data provide strong evidence that RCI-utilizing and RCII-utilizing Chloroflexia members inherited phototrophy from a most recent common phototrophic ancestor. The Chloroflexota phylum preserves an evolutionary record of the use of contrasting phototrophic modes among genetically related bacteria, giving new context for exploring the diversification of phototrophy on Earth.
Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array
Authors: Hiranuma Minami , Okuda Yuichi , Fujii Yuuka , Richard Jean-Philippe , Watanabe Tomohisa 
Published: March 2024

AbstractSensory neurons are afferent neurons in sensory systems that convert stimuli and transmit information to the central nervous system as electrical signals. Primary afferent neurons that are affected by non-noxious and noxious stimuli are present in the dorsal root ganglia (DRG), and the DRG sensory neurons are used as an in vitro model of the nociceptive response. However, DRG derived from mouse or rat give a low yield of neurons, and they are difficult to culture. To help alleviate this problem, we characterized human induced pluripotent stem cell (hiPSC) derived sensory neurons. They can solve the problems of interspecies differences and supply stability. We investigated expressions of sensory neuron related proteins and genes, and drug responses by Multi-Electrode Array (MEA) to analyze the properties and functions of sensory neurons. They expressed nociceptor, mechanoreceptor and proprioceptor related genes and proteins. They constitute a heterogeneous population of their subclasses. We confirmed that they could respond to both noxious and non-noxious stimuli. We showed that histamine inhibitors reduced histamine-induced neuronal excitability. Furthermore, incubation with a ProTx-II and Nav1.7 inhibitor reduced the spontaneous neural activity in hiPSC-derived sensory neurons. Their responsiveness was different from each drug. We have demonstrated that hiPSC-derived sensory neurons combined with MEA are good candidates for drug discovery studies where DRG in vitro modeling is necessary.
Dual-band dual-polarized sub-6 GHz phased array antenna with suppressed higher order modes
Authors: Barad Debaprasad , Dash Jogesh Chandra , Sarkar Debdeep , Srinivasulu P. 
Published: March 2024

AbstractThis manuscript proposes a dual-band dual-polarized 64-element phased array antenna (PAA) with suppressed higher order modes (HoMs). Each array element is a microstrip patch structure with complementary split ring resonator (CSRR) loading, allowing for simultaneous dual-band and dual-polarization performance at 3.45 GHz and 3.87 GHz. The proposed PAA exhibits impedance matching of $$\le - 20$$

≤
-
20

dB at 3.45 GHz and 3.87 GHz while limiting the mutual coupling below $$- 19$$

-
19

dB between the adjacent elements at both the operating bands. The proposed design includes a plated through hole placed near the CSRR loading to suppress any higher-order modes (HOMs). Two antenna prototypes, without and with HOMs suppression techniques are fabricated and measured. In addition, we demonstrate the beam steering of the proposed PAA up to $$\pm 60^0$$

±

60
0

using an off-the-shelf 6-bit phase shifter module. The proposed 64 -element array achieves a gain of 23 dBi, and shows a minimal steering loss of $$\simeq 3$$

≃
3

dB over the steering angle.
Association between chronic obstructive pulmonary disease and in-hospital mortality after percutaneous coronary intervention: a retrospective cohort study in Germany
Authors: Hochhausen Nadine , Mechelinck Mare , Billig Sebastian , Rossaint Rolf , Kork Felix 
Published: March 2024

AbstractChronic obstructive pulmonary disease (COPD) is one of the leading chronic diseases worldwide. However, the impact of COPD on outcome after percutaneous coronary intervention (PCI) remains unclear. In this retrospective cohort study, we analyzed the data of hospitalized patients undergoing PCI in Germany between 2015 and 2019. We compared in-hospital mortality, hospital length of stay and peri-interventional ventilation time (VT) in patients with and without COPD, including different COPD severity grades, COPD with exacerbation (COPDe) and infection (COPDi). We analyzed the data of 3,464,369 cases undergoing PCI. A total of 291,707 patients (8.4%) suffered from COPD. Patients suffering from COPD died more often (2.4% vs. 2.0%; p < 0.001), stayed longer hospitalized (5 days (2–10) vs. 3 days (1–6); p < 0.001), were more frequent (7.2% vs. 3.2%) and longer ventilated (26 h (7–88) vs. 23 h (5–92); p < 0.001). Surprisingly, COPD was associated with a 0.78-fold odds of in-hospital mortality and with reduced VT (− 1.94 h, 95% CI, − 4.34 to 0.43). Mild to severe COPD was associated with a lower risk of in-hospital mortality and reduced VT, whereas very severe COPD, COPDe and COPDi showed a higher risk of in-hospital mortality. We found a paradoxical association between mild to severe COPD and in-hospital mortality, whereas very severe COPD, COPDe and COPDi were associated with higher in-hospital mortality. Further investigations should illuminate, whether comorbidities affect these associations.
Sulfamethoxazole-induced crystal nephropathy: characterization and prognosis in a case series
Authors: Azencot Ruben , Saint-Jacques Camille , Haymann Jean-Philippe , Frochot Vincent , Daudon Michel , Letavernier Emmanuel 
Published: March 2024

AbstractCotrimoxazole (Trimethoprim/Sulfamethoxazole-SMX) is frequently used in critically ill and immunocompromised patients. SMX is converted to N-acetyl-sulfamethoxazole (NASM) and excreted by the kidneys. NASM may form crystals in urine, especially in acid urine, that may induce a crystalline nephropathy. However, the imputability of crystals in acute kidney injury (AKI) has not been proven. We aimed to assess whether NASM crystals may promote AKI and to investigate risk factors associated with NASM crystalline nephropathy. Patients from Ile-de-France, France who developed AKI under SMX treatment introduced during hospitalization and had a crystalluria positive for NASM crystals were selected. Patients with excessive preanalytical delay for crystalluria or missing data regarding SMX treatment were excluded. We used the Naranjo score to assess the causal relationship between SMX and the development of AKI in patients with positive NASM crystalluria. Fourteen patients were included. SMX was the probable cause of AKI for 11 patients and a possible cause for 3 patients according to Naranjo score. Patients were exposed to high doses of SMX (but within recommended ranges), and most of them had a preexisting chronic kidney disease and were hypoalbuminemic. Urine pH was mildly acid (median 5.9). AKI occured more rapidly than expected after introduction of SMX (median 4 days) and recovered rapidly after drug discontinuation in most, but not all, cases. SMX is a probable cause of crystalline nephropathy. Monitoring of crystalluria in patients exposed to SMX may be of interest to prevent the development of crystalline nephropathy. Approval number of the study: BPD-2018-DIAG-008.
Factors associated with the SARS-CoV-2 immunoglobulin-G titer levels in convalescent whole-blood donors: a Chinese cross-sectional study
Authors: Tan Donglin , Du Xinman , Tang Jingyun , Liu Humin , Li Meng , Kang Jianxun , Li Xiaochun , Li Ying , Luo Yue , Wang Qing , Gu Xiaobo , Zhao Zonghan , Fu Xuemei , Chen Xue 
Published: March 2024

AbstractBlood transfusions from convalescent Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infected patients could be used to treat patients with severe infections or immunocompromised patients. However, it is necessary to select the optimal donors to maximize the utilization of resources. In this study, we investigated the associations among body mass index (BMI), tobacco smoking, exercise frequency and duration, and alcohol consumption with the SARS-CoV-2 immunoglobulin-G (IgG) antibody titer levels with in the Chinese convalescent blood donor population. Here we show that BMI, smoking habits, and exercise frequency appear to be predictive factors for IgG levels in convalescent male blood donors. However, these variables were not observed as predictive of IgG levels in female convalescent blood donors. The findings could be used to optimize the screening for potential blood donors to treat immunocompromised or severely ill COVID-19 patients.
Discussing the safety and effectiveness of transcatheter arterial embolization combined with intravenous chemotherapy in treating locally advanced breast cancer
Authors: Zhang Jingjun , Zhang Jie , Niu Xiangke , Zhou Yongxiang , Guo Yufeng , Wang Yuanzhi , Shou Feng 
Published: March 2024

AbstractTo investigate the efficacy and safety of drug-eluting bead-transarterial chemoembolization (DEB-TACE) combined with systemic chemotherapy in HR+/Her2− locally advanced breast cancer (LABC) patients. A controlled study was conducted on LABC patients treated at Jianyang People’s Hospital and the First Affiliated Hospital of Chengdu Medical College from December 2020 to June 2022. The patients were randomly divided into the experimental group and the control group. The experimental group received DEB-TACE combined with the TAC regimen (175 mg/m2 paclitaxel-loaded albumin, 50 mg/m2 Doxorubicin, and 500 mg/m2 cyclophosphamide), while the control group received the TAC regimen intravenously. The therapeutic efficacy was evaluated using the mRECIST criteria. Statistical analysis was performed using SPSS 22.0 software, and baseline characteristics, overall response rate (ORR), pathological complete response (PCR), adverse reactions, and complications were compared between the two groups using paired t-test and chi-square test. A total of 60 patients were included, with 30 patients in the experimental group (50%) and 30 patients in the control group (50%). After the first treatment, the ORR was 90% in the experimental group and 60% in the control group (P < 0.05). The overall ORR was 100% in the experimental group and 83% in the control group (P < 0.05). PCR was achieved in 14 patients (47%) in the experimental group and 4 patients (13%) in the control group. The main adverse reactions in the experimental group were skin blistering, pigmentation, and pain. There was no statistically significant difference in vomiting and grade II or above bone marrow suppression between the two groups. No grade III or above adverse events occurred in either group. The comparison of tumor shrinkage between the two groups was P = 0.051, and axillary lymph node shrinkage was P < 0.05. The use of drug-eluting beads in combination with neoadjuvant chemotherapy is a feasible and safe treatment option for locally advanced breast cancer patients.
Logistic analysis of delayed reporting of emergency blood potassium and comparison of improved outcomes
Authors: Zhang Jian , Lv Shuangshuang , Jin Tingting , Hu Xiaxuan 
Published: March 2024

AbstractPotassium testing is an essential test in emergency medicine. Turnaround time (TAT) is the time between specimen receipt by the laboratory and the release of the test report. A brief in-laboratory TAT increases emergency department effectiveness. Optimizing processes to shorten TAT using other tools requires extensive time, resources, training, and support. Therefore, we aimed to find a convenient way to shorten TAT, identify risk factors affecting the timeliness of emergency potassium test reporting, and verify the intervention’s effects. The dependent variable was emergency potassium reporting time > 30 or < 30 min. Logistic analysis was performed on monitorable factors, such as sex, age, potassium results, number of items, specimen processing time (including centrifugation and time before specimen loading), critical value ratio, instrument status, shift where the report was issued, specimen status, and work experience, as independent variables. In the multivariate analysis, work experience, instrument failure rate, and specimen processing time were risk factors for emergency blood potassium reporting exceeding 30 min. Improvement measures were implemented, significantly decreasing the timeout rate for acute potassium reporting. Our study confirms the usefulness of logistics in reducing the time required to report potassium levels in the emergency department, providing a new perspective on quality management.