bluuebunny/crossref_metadata_2025_split huggingface dataset:
I REGION ASSOCIATED HISTOINCOMPATIBILITY WITH ABSENCE OF ACCELERATED REJECTION OF SECOND SET SKIN GRAFTS DETECTED IN TESTS WITH A NEW HAPLOTYPE, H‐2dx
Authors: Zachařová Gisela , Renčková Ľuba , Dux Anna , Démant P. 
Published: April 2007

SUMMARYH‐2 typing of two inbred mouse strains, GRS I/A and LIS/A, revealed that they both carry the same, previously unknown haplotype, designated H‐2dx. The H‐2dx haplotype has the K region allele identical or very similar to that of H‐2d, however it differs from H‐2d at the I region genes and has none of the D region alleles previously described in inbred mouse strains.When the H‐2dx haplotype was used in F1 complementation tests, it failed to prevent rejection of B10.D2 skin grafts by (GR × B10.A)F1 and (LI × B10.A)F1 recipients, as well as rejection of R103 grafts by (GR × 2R)F1, (LI × 2R)F1, (GR × B10)F1, and (LI × B10)F1 recipients. In all these combinations, the donor and the recipients were matched at the K and D regions, but differed at the I region. The mean rejection time was 12 days (beginning) and 15.9 days (end of rejection).The second set skin grafts were rejected at approximately the same speed as the first set skin grafts, and after their rejection a cytotoxic antibody, most likely anti‐Ia, was found in the serum of the recipient mice. The absence of accelerated second set skin graft rejection in the I region incompatibility observed in these experiments is in sharp contrast to the extremely fast rejection of K or D region incompatible second set skin grafts. This lack of demonstrable anamnestic response is either due to the presence of enhancing antibodies, or to an inherently inferior capacity of I region incompatibility to elicit, in vivo, an effective anamnestic response.
ACTIVATED T LYMPHOCYTES EXPRESS NEW SURFACE DETERMINANTS WHICH REACT WITH HETEROLOGOUS ANTI‐B CELL SERUM
Authors: Schirrmacher V. , Festenstein H. 
Published: April 2007

SUMMARYSurface determinants on activated mouse T lymphocytes were investigated in a new radioassay. Activated cells were selectively labelled with 14C‐thymidine and then treated with specific antibody and complement followed by trypsinization to disrupt the membranes and release radiolabelled DNA from specifically lysed cells (14C‐thymidine release assay).T lymphocytes were activated in vitro (MLC and PHA) or in vivo (skin sensitization with oxazolone) and characterized by their sensitivity to the cytotoxic effect of anti‐θ serum. These cells were resistant to the cytotoxic effect of R anti‐MIg, a purified polyvalent anti‐mouse immunoglobulin serum, but were lysed by treatment with R anti‐MBLA, a rabbit anti‐mouse B lymphocyte serum. This cytotoxic activity could be absorbed with spleen cells from nude (nu/nu) mice but not with thymocytes. Furthermore, pretreatment of lymph node cells with R anti‐MBLA and complement before stimulation with PHA led to an increased PHA response, indicating that the serum reacted with normal B but not T lymphocytes. When these activated purified T lymphocytes were treated with R anti‐MBLA, as much 14C‐thymidine was released from these cells as from unpurified control cells (>70%). The percentage of 14C‐thymidine released from activated T cells by treatment with either anti‐θ serum or R anti‐MBLA was surprisingly similar and no significant additive effect was observed when the cells were treated with both antisera together. LPS activated B lymphocytes showed a different reactivity pattern with the antisera: they were lysed by R anti‐MBLA but not by anti‐θ serum while R anti‐MIg had an intermediate cytotoxic effect.An interpretation of these findings is offered which suggests that transformation of T lymphocytes is accompanied by derepression of certain genetic information and by the expression of new surface determinants, some of which may be identical with determinants which are otherwise expressed only on normal B lymphocytes.
HIGH DENSITY LIPOPROTEIN (HDL) POLYMORPHISMS IN RABBIT I. A COMPARATIVE STUDY OF RABBIT AND HUMAN SERUM HIGH DENSITY LIPOPROTEIN
Authors: Børresen Anne‐Lise 
Published: April 2007

SUMMARYDifferent classes of rabbit serum lipoprotein were prepared by ultracentrifugal flotation at densities 1·006, 1·063 and 1·21 g/ml. Agarose gel electrophoresis on rabbit whole serum and the serum fractions with different densities showed that this technique separates the different lipoprotein classes reasonably well.The electrophoretic mobility of the different lipoprotein classes of rabbit serum seems to be similar to that of the human lipoproteins, with the exception of α1‐lipoprotein which had a greater mobility than human α1‐lipoprotein.The chemical composition of rabbit high density lipoprotein (HDL) was fairly similar to that of human HDL although the former seems to be richer in triglycerides.HDL was, after isolation by ultracentrifugal flotation at density 1·21, delipidated and submitted to gel filtration on Sephadex G‐200 in 8 m urea. The major protein fraction of rabbit apo HDL corresponds in elution volume to that of the major fraction of human apo HDL, apoA‐I. A protein fraction corresponding to human apoA‐II does not seem to be present in rabbit HDL in demonstrable amounts. The rabbit protein fraction sometimes appearing in the area corresponding to human apoA‐II could not be found to be affected by the reduction and alkylation method after which humana poA‐II splits into two identical chains.
JUVENILE DIABETES MELLITUS: HLA‐ANTIGEN FREQUENCIES DEPENDENT ON THE AGE OF ONSET OF THE DISEASE
Authors: Schernthaner G. , Ludwig H. , Mayr W. R. 
Published: April 2007

SUMMARYA close correlation between juvenile diabetes mellitus and HLA‐B8, HLA‐BW15 and HLA‐CW3 was found. Association of these antigens with juvenile diabetes mellitus was closely dependent on the ages of onset of the disease. Frequencies of BW15 and CW3 showed a remarkably low incidence in the childhood diabetics (0–15 years old) and were found increased in the patients with later (16 years or older) onset diabetes. HLA‐B8 frequencies were found increased in all the groups of diabetics with different ages of onset. These findings point to the importance of HLA‐B8 in childhood and HLA‐B8, BW15 and CW3 in the later onset juvenile onset diabetes mellitus (JOD).
GENETIC STUDIES IN INBRED RATS VII. TENTATIVE MODEL FOR THE MAJOR HISTOCOMPATIBILITY COMPLEX
Authors: iii Gill Thomas J. , Kunz Heinz W. 
Published: April 2007

SUMMARYSeveral lines of evidence are presented which support a tentative model of the major histocompatibility complex (MHC) of the rat. The serologically defined (SD), lymphocyte defined (LD) and immune response (Ir) loci are separate but linked, and there are probably two loci controlling the serologically defined antigens. The MHC in the rat appears to resemble that in the human more than that in the mouse.
LINKAGE RELATIONSHIPS OF THE LOCI OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN FAMILIES WITH A RECOMBINATION IN THE HLA REGION
Authors: Bijnen A. B. , Schreuder Ieke , Khan P. Meera , Allen F. H. , Giles C. M. , Los W. R. T. , Volkers W. S. , van Rood J. J. 
Published: April 2007

SUMMARYA number of families with an established recombination in the major histo‐compatibility complex has been investigated for markers known to be coded by genes of this linkage group. The results provide further data on the relative position of the loci for HLA‐A, HLA‐B, HLA‐C, HLA‐D, Bf, Chido, Rodgers and PGM3 on chromosome 6. A positive lodscore for linkage between HLA and blood group P was found; lodscores between HLA and nineteen other markers were negative.
HL‐A8 AND THE IMMUNE RESPONSE TO VARIOUS ANTIGENS
Authors: Scott B. B. , Cooke E. Mary , Hambling M. H. , Rajah S. M. , Swinburne M. L. , Losowsky M. S. 
Published: April 2007

SUMMARYThe possession of the histocompatibility antigen HL‐A8 has been correlated with serum levels of antibodies to gluten and a variety of other dietary, viral, and bacterial antigens and autoantigens. The only significant correlation with HL‐A8 was of raised levels of gluten antibody. Thus it seems that HL‐A8 may be associated with an enhanced immune response specifically to gluten, and not with a generally enhanced immunological responsiveness as has been suggested.
HL‐A ANTIGENS IN JUVENILE RHEUMATOID ARTHRITIS
Authors: Macurová Helena , Ivašková Eva , Havelka S. , Iványi P. 
Published: April 2007

SUMMARYHL‐A antigens were examined in sixty‐two patients, thirty‐one boys and thirty‐one girls, diagnosed as having JRA. HL‐A 27 was the only antigen with a significantly increased frequency. This increase concerned predominantly male patients in whom the disease developed at the beginning of puberty. In this group of sixteen boys, the polyarticular form was more frequent and tended to be associated with sacroiliitis, while the rheumatoid factor was negative in all but one. The frequency of HL‐A 27 in this group was 62.5%.The patients were also examined by the lymphotoxic test with anti‐H‐2 allo‐immune sera known to exhibit a high degree of association with some HL‐A antigens. On JRA lymphocytes these associations were confirmed for HL‐A 2 and anti‐H‐2f and for HL‐A 27 and anti‐H‐2p. The strongest reactions were observed with lymphocytes from male patients around the age of puberty. These data indicate that steroid hormones influence the expressivity of some HL‐A‐associated cell plasma membrane structures.
EXPERIMENTAL AUTOIMMUNE ENCEPHALO‐MYELITIS IN MICE: GENETIC CONTROL OF SUSCEPTIBILITY*
Authors: Bernard C. C. A. 
Published: April 2007

SUMMARYExperimental autoimmune encephalomyelitis (EAE) was induced in inbred and congenic strains of mice by injection of mouse spinal cord homogenate (MSCH) in Freund's complete adjuvant (FCA) with pertussis vaccine. Genetic analyses showed that susceptibility to EAE in mice was inherited as a dominant trait and was in part controlled by genes located in the centromeric half of the H‐2 complex.Mice with EAE developed cell‐mediated immune responsiveness to basic protein of myelin (BPM), as judged by the macrophage migration inhibition assay, using peritoneal exudate cells; this was not observed with mice of resistant strains. However, the migration of peritoneal exudate cells of both susceptible and resistant strains was significantly inhibited in the presence of purified protein derivative (PPD) of M. tuberculosis. Thus, the genes involved in the control of susceptibility to EAE also influence T cell responsiveness to BPM. Antibody to BPM, as judged by radioimmunoassay, was detected in susceptible and resistant strains but there was no correlation between the presence or levels of antibody and susceptibility or resistance to EAE. It is suggested that resistance to EAE is associated with failure of T cells to recognize and/or respond to the encephalitogenic determinant of the BPM molecule.
STUDIES ON THE MEMBRANE GLYCOPROTEIN DEFECT OF En(a‐) ERYTHROCYTES
Authors: Dahr W. , Uhlenbruck G. , Leikola J. , Wagstaff W. , Landfried K. 
Published: April 2007

SUMMARYDiscontinuous sodium dodecylsulphate‐polyacrylamide gel electrophoresis, followed by periodic acid/Schiff or Coomassie staining and densitometry, spectrophotometric and gas‐liquid chromatographic carbohydrate analyses as well as heterophile agglutinins are employed to study the nature of the membrane glycoprotein defect in En(a–) erythrocytes from Finland and England, heterozygous Ena red cells from Finland and the erythrocytes of two individuals from Switzerland. The results suggest that En(a–) cells lack the major membrane sialoglycoprotein, the so‐called MN glycoprotein. Heterozygous Ena erythrocytes from Finland and those from Switzerland have only about half of the normal amount of MN glycoprotein. The molecular weight of the major Coomassie staining membrane protein (component III) is increased by approx. 5000 and 3000 daltons in En(a–) and the other red cells respectively. Some aspects of this membrane defect are discussed.
STUDIES ON H‐2 SPECIFICITIES ON MOUSE TUMOUR CELLS BY A NEW MICRORADIOASSAY*
Authors: Garrido F. , Schirrmacher V. , Festenstein H. 
Published: April 2007

SUMMARYSeven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty‐four well defined H‐2 allo‐antisera and anti‐Thy 1.2. We used a new radioassay that involves antibody‐complement treatment of the tumour target cells followed by postlabelling the surviving tumour cells with 14C‐thymidine. With a relatively high frequency the anti‐H‐2 sera were reacting differently with the tumour cells than with respective tive syngeneic lymphoid cells. Thirty‐six anomalous reactions out of 129 investigated were detected. Absorbtion experiments performed with H‐2 antigen positive or negative lymphoid cells revealed a striking similarity between these extra‐specificities and H‐2 specificities of foreign haplotypes. The results are discussed in relation to the biological importance of the extra‐specificities, both with regard to origin (derepression) and function (transplantation antigen properties).
MULTIPLE FOREIGN NON‐H‐2 DETERMINANTS ON THE SURFACE OF A CHEMICALLY‐INDUCED MURINE SARCOMA*
Authors: Invernizzi G. , Carbone G. , Meschini Angela , Parmiani G. 
Published: April 2007

SUMMARYBy immunizing BALB/c (H‐2d mice against normal tissues from C57BL/6J (H‐2b), C3Hf (H‐2k) and DBA/2 (H‐2d), but not from AKR (H‐2k) strains, resistance was induced to the subsequent challenge of the ‘syngeneic’ methyl‐cholanthrene‐induced BALB/c sarcoma ST5; lymph node cells from allo‐immune BALB/c mice were also able to exert a parallel cytotoxic effect against in vitro cultured ST5 cells. The involvement of foreign H‐2 specificities in the observed cross‐reactions was ruled out by absorptions of H‐2 monospecific sera and by the interallelic combinations used, thus suggesting that non‐H‐2 histocompatibility antigens were responsible for the above findings.By using the indirect isotopic antiglobulin assay, BALB/c anti‐C57BL/6J and anti‐C3Hf polyspecific sera were found to bind specifically to cultured ST5 cells. C57BL/6J and C3Hf, but not DBA/2, lymph node cells were able to absorb the anti‐ST5 activity of the anti‐C57BL/6J serum. These results indicated that ST5 cells expressed on their surface at least two different sets of foreign non‐H‐2 antigens: one shared by C57BL/6J and C3Hf tissues, and detected by both cell‐mediated and serological techniques; the other one belonging to DBA/2 tissues, and revealed mainly at the cell‐mediated level.
COINCIDENT EFFECT IN THE GRAFT‐VERSUS‐HOST REACTION OF BALB/c LYMPHOID CELLS DERIVED FROM MICE IMMUNE EITHER TO ALLOGENEIC NORMAL TISSUE OR TO SYNGENEIC CHEMICALLYINDUCED FIBROSARCOMATA
Authors: Parmiani G. , Invernizzi G. 
Published: April 2007

SUMMARYBy using the graft‐versus‐host reaction (GVHR) based on the spleen enlargement test of Simonsen (1962), lymphocytes from BALB/c mice immune to syngeneic ST2 or ST5 sarcomata were shown to behave like BALB/c anti‐DBA/2 lymphocytes in specifically increasing the GVHR in (BALB/c × DBA/2)F1 newborns as compared with non‐immune BALB/c cells. No such effect was evident when BALB/c anti‐ST2 or anti‐ST5 lymphoid cells were given to (BALB/c × C3Hf)F1 and to (BALB/c × AKR)F1 mice; anti‐ST5 lymphocytes were also ineffective into (BALB/c × C57BL/6)F1 newborns in which anti‐ST2 lymphocytes were able to increase the GVHR over the background induced by non‐immune BALB/c cells. A third immunogenic BALB/c fibrosarcoma C‐1 was able to activate syngeneic lymphocytes which gave an augmented GVHR in (BALB/c × C3Hf)F1 and (BALB/c × AKR)F1 but not in the other hybrids. The efficacy of BALB/c anti‐ST2 or ‐ST5 lymphocytes in reproducing a BALB/c anti‐DBA/2 like anamnestic response in (BALB/c × DBA/2)F1 mice support previous findings indicating that non‐H‐2 alien histocompatibility antigens of the DBA/2 strain, are expressed on these tumour cells. The GVHR induced by lymphoid cells immune to C‐1 tumour are also in keeping with serological and transplantation studies (reported elsewhere) indicating the expression of foreign H‐2k antigens on this sarcoma.
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS IN MICE: ANTIGEN BINDING LYMPHOCYTES TO BASIC PROTEIN OF MYELIN IN SUSCEPTIBLE AND RESISTANT STRAINS
Authors: Bernard C. C. A. , Roberts Isabel M. , Mackay I. R. 
Published: April 2007

SUMMARYAntigen‐binding lymphocytes (ABL) to basic protein of myelin (BPM) were demonstrable in the thymus and spleen of various strains of mice. Counts of ABL did not differ in strains resistant and susceptible to EAE, and did not differ when human or murine 125I‐labelled BPM was used as a labelled antigen to detect ABL. After injection with mouse spinal cord and adjuvants, under conditions known to be immunogenic and encephalitogenic for susceptible strains of mice, counts of ABL increased two‐ to four‐fold in susceptible strains but did not increase in resistant strains. Antigen binding could not be accounted for by the attachment to cells of cytophilic antibody with specificity for BPM, because such antibody was seldom demonstrable even after immunization. Inferences from the study were: (i) that tolerance (non‐responsiveness) to certain autoantigens at least must depend on control over continually present antigen‐reactive lymphocytes rather than on any lack of capacity to recognize antigen; (ii) differences in susceptible and resistant strains are evident after, rather than before, immunization.
HUMAN IgG3 ALLOTYPES, WITH SPECIAL REFERENCE TO A NEW ALLOTYPE RELATED TO G3m(g) (G3m21)
Authors: Loghem Erna van , Blanc Madeleine , Lange Gerda De 
Published: April 2007

SUMMARYThe IgG3 allotype described as L1 (Blanc et al., 1976) occurs on the CH3 region of G3m(g) proteins, in contrast to G3m(g) that is known to be present on the CH2 region. G3m(g) and L1 are, as a rule, present on the same γ3 heavy chain, just like the G3m(b) subspecificities of the CH2 region, (b1) and (b4), with those of the CH3 region (b0), (b3) and (b5).Several families were investigated that showed inheritance of rare combinations of IgG3 allotypes. The data obtained are suggestive for notation of L1 as (g5), since L1 probably occupies a position antithetical to (b5).The relation of amino acid substitutions to allotypes and isoallotypes is discussed.
LINKAGE STUDIES WITH C6
Authors: Hobart M. J. , Cook P. J. L. , Lachmann P. J. 
Published: April 2007

SUMMARYThe common structural variants of C6 have been used to study the linkage relations of the locus in human pedigrees. Linkage between C6 and RH, Fy, ACP1, MNSs, Jk, HLA, ABO, ESD, Hp, ADA, GPT, Gc, Pi, Gm and Km has been excluded at recombination fractions at least as great as 0.1 in the male.
THE DISTRIBUTION OF THE INHERITED Hl 1 AND R 67 ANTIGENS ON RABBIT SERUM HIGH DENSITY LIPOPROTEIN (HDL) PARTICLES
Authors: Børresen Anne‐Lise 
Published: April 2007

SUMMARYQualitative and quantitative analysis of the distribution of Hl 1 antigen and R 67 antigen on high density lipoprotein (HDL) particles were conducted on six rabbit sera. The Hl 1 antigen showed non‐identity with A‐I‐containing HDL particles, and antiserum to Hl 1 did not remove A‐I‐containing HDL particles. The R 67 antigen showed partial identity with A‐I‐containing HDL particles. Antiserum to R 67 antigen removed 30–40% of A‐I‐containing HDL particles from the sera of animals which were heterozygous for R 67, and 65% of the A‐I‐containing HDL particles from the serum of one R 67 homozygous animal.
INTERCELLULAR ADHESIVENESS OF H‐2 IDENTICAL AND H‐2 DISPARATE CELLS
Authors: Zelený V. , Matoušek V. , Lengerová Alena 
Published: April 2007

SUMMARYUsing a 2 times 2 design, the collection rates of cells from radioactively labelled single‐cell suspensions on cell‐coated collecting surfaces were tested for a possible H‐2 effect on intercellular adhesiveness. By a statistical procedure, the undesirable variability in the suspension and/or in the collecting surface could be eliminated, and the H‐2 specific net effect estimated. In this way it was possible to detect (even on the background of a large non‐specific variability) significant reductions of the allogeneic collection rates (i.e. between cells which differed only in their H‐2 haplotypes) as compared to the syngeneic standards. It is concluded that cells of a given H‐2 haplotype have preferential adhesiveness to syngeneic cells.
PARTIAL PURIFICATION AND CHARACTERIZATION OF THE INHERITED H1 1 AND R 67 ANTIGENS OF RABBIT SERUM HIGH DENSITY LIPOPROTEIN
Authors: Børresen Anne‐Lise , Kindt T. J. , Berg K. 
Published: April 2007

SUMMARYPurification and characterization of the Hl 1 antigen revealed a polypeptide with molecular weight of 20,000 by gel filtration. The peptide contained 20% neutral sugar and 4.5% neuraminic acid. Amino acid analysis as well as the Nterminal sequence for thirteen amino acid residues and three C‐terminal amino acids were determined. Difference index analysis gave an indication that the H1 1 polypeptide is unique.Purification of R 67 antigen did not give a pure polypeptide in sufficient amount to do chemical analysis. A crude fraction of R 67 antigen had three bands on SDS‐PAGE, all with R67 antigenic activity. The crude fraction contained 10% neutral sugar and 3.5% neuraminic acid. No N‐ or C‐terminal amino acids could be determined for the crude fraction of R 67 antigen.
MEASLES ANTIBODIES AND HLA
Authors: Haverkorn M. J. , Norrby E. 
Published: April 2007

SUMMARYBlood samples were collected from healthy twin pairs of the same sex, living in the same households and attending the same schools. The occurrence of HLA antigens and seven red cell antigens was determined. The serum titres of antibodies against three different components of measles virus were measured by haemagglutination inhibition, by haemolysis inhibition and by nucleocapsid complement fixation tests. The variability of titres within twin pairs was significantly greater among seventy dizygotic twins than among seventy monozygotic twins, suggesting an influence of genetic factors on the measles antibody response. However, no evidence for a genetic linkage between HLA loci and the loci involved in the antibody response was apparent, since the variability of antibody titres among eighteen dizygotic pairs with common HLA haplotypes was about similar to that among twenty‐three pairs with different haplotypes. A possible association between antibody titres and HLA‐A3 and B7 antigens was also analysed in the present material. The comparisons were made between groups of single individuals in the twin pairs. The presence or absence of HLA‐A3 antigen did not significantly influence the mean titres of groups. However, in two out of six comparisons, the mean antibody titre was significantly higher in the group of individuals who had HLA‐B7.
THE EFFECT OF ENZYMES ON THE BLOOD FACTORS ASSOCIATED WITH THE FV SYSTEM OF BOVINE ERYTHROCYTES
Authors: Sellei J. 
Published: April 2007

SUMMARYTreatment of cattle red cells (CRC) with proteases and neuraminidase revealed at least one trypsin‐ or pronase‐sensitive type and a protese‐resistant type of sialoglycoprotein.The antigen F itself and the antigen IM in the F/F and F/V type of CRC were associated with the protease‐resistant glycoprotein. Moreover, they were demonstrable in the agglutination test, but only after treatment with proteases. The antigen V, allelic to F, was, however, associated with a pronase‐sensitive protein and the antigen IM in the V/V type of CRC with a trypsin‐ or pronasesensitive glycoprotein. The antigen N′ was inactivated by chymotrypsin or pronase, irrespective of whether it was transmitted with the F or with the V.While the antigens IM, N′ and the antigen reacting with rabbit serum, in addition to the antigen F in the F/F type of CRC, showed no or only very weak (titre 1:2) reactions, these same antigens, in addition to the antigen V in the V/V type of CRC, all showed high titres (1: 32 to 1: 128) in the anti‐globulin test.