bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin protection creams in medical settings: successful or evil?
Authors: Xhauflaire-Uhoda Emmanuelle , Macarenko Elena , Denooz Raphaël , Charlier Corinne , Piérard Gérald E 
Published: July 2008

AbstractBackgroundChronic exposure to mild irritants including cleansing and antiseptic products used for hand hygiene generates insults to the skin. To avoid unpleasant reactions, skin protection creams are commonly employed, but some fail to afford protection against a variety of xenobiotics. In this study, two skin protection creams were assayed comparatively looking for a protective effect if any against a liquid soap and an alcohol-based gel designed for hand hygiene in medical settings.MethodsCorneosurfametry and corneoxenometry are two in vitro bioessays which were selected for their good reproducibility, sensitivity and ease of use. A Kruskal-Wallis ANOVA test followed by the Dunn test was realized to compare series of data obtained.ResultsSignificant differences in efficacy were obtained between the two assayed skin protection creams. One of the two tested creams showed a real protective effect against mild irritants, but the other tested cream presented an irritant potential in its application with mild irritants.ConclusionThe differences observed for the two tested skin protection creams were probably due to their galenic composition and their possible interactions with the offending products. As a result, the present in vitro bioassays showed contrasted effects of the creams corresponding to either a protective or an irritant effect on human stratum corneum.
Metabolic modelling of polyhydroxyalkanoate copolymers production by mixed microbial cultures
Authors: Dias João ML , Oehmen Adrian , Serafim Luísa S , Lemos Paulo C , Reis Maria AM , Oliveira Rui 
Published: July 2008

Abstract

Background
This paper presents a metabolic model describing the production of polyhydroxyalkanoate (PHA) copolymers in mixed microbial cultures, using mixtures of acetic and propionic acid as carbon source material. Material and energetic balances were established on the basis of previously elucidated metabolic pathways. Equations were derived for the theoretical yields for cell growth and PHA production on mixtures of acetic and propionic acid as functions of the oxidative phosphorylation efficiency, P/O ratio. The oxidative phosphorylation efficiency was estimated from rate measurements, which in turn allowed the estimation of the theoretical yield coefficients.

Results
The model was validated with experimental data collected in a sequencing batch reactor (SBR) operated under varying feeding conditions: feeding of acetic and propionic acid separately (control experiments), and the feeding of acetic and propionic acid simultaneously. Two different feast and famine culture enrichment strategies were studied: (i) either with acetate or (ii) with propionate as carbon source material. Metabolic flux analysis (MFA) was performed for the different feeding conditions and culture enrichment strategies. Flux balance analysis (FBA) was used to calculate optimal feeding scenarios for high quality PHA polymers production, where it was found that a suitable polymer would be obtained when acetate is fed in excess and the feeding rate of propionate is limited to ~0.17 C-mol/(C-mol.h). The results were compared with published pure culture metabolic studies.

Conclusion
Acetate was more conducive toward the enrichment of a microbial culture with higher PHA storage fluxes and yields as compared to propionate. The P/O ratio was not only influenced by the selected microbial culture, but also by the carbon substrate fed to each culture, where higher P/O ratio values were consistently observed for acetate than propionate. MFA studies suggest that when mixtures of acetate and propionate are fed to the cultures, the catabolic activity is primarily guaranteed through acetate uptake, and the characteristic P/O ratio of acetate prevails over that of propionate. This study suggests that the PHA production process by mixed microbial cultures has the potential to be comparable or even more favourable than pure cultures.
Flexion reminder device to discourage recurrent posterior dislocation of a total hip replacement: a case report
Authors: Wong King , Sivan Manoj , Matthews Gordon 
Published: July 2008

Abstract

Introduction
Recurrent dislocation of a total hip replacement prosthesis is a frustrating complication for both the surgeon and the patient. For positional dislocations with no indications for revision surgery, the current best treatment is physiotherapy, the use of abduction braces and avoidance of unsafe hip positions. Abduction braces can be cumbersome and have poor compliance. We report the successful use of a new lightweight flexion reminder device that can be used to treat people with this condition.

Case presentation
A 64-year-old British woman experienced recurrent positional posterior dislocation after primary hip replacement, particularly when involved in activities involving unsafe flexion of the operated hip. She disliked using an abduction brace and hence was given a simple 'flexion reminder device' that could be strapped to the thigh. Beyond the safe flexion limit, the padded top end of the device hitched against the groin crease and reminded her not to flex further, to avoid dislocation. She experienced no discomfort in wearing the device continuously throughout the day and was very satisfied. She has had no further dislocations in the 2 years since she began using it.

Conclusion
In cases of arthroplasty dislocation caused mainly by an unsafe hip position, and with no indication for revision surgery, this new lightweight and easily worn flexion reminder device may be a good option for avoiding such positional dislocations, particularly those caused by unsafe flexion.
Mosaicism for r(X) and der(X)del(X)(p11.23)dup(X)(p11.21p11.22) provides insight into the possible mechanism of rearrangement
Authors: Shchelochkov Oleg A , Cooper M Lance , Ou Zhishuo , Peacock Sandra , Yatsenko Svetlana A , Brown Chester W , Fang Ping , Stankiewicz Pawel , Cheung Sau Wai 
Published: July 2008

Abstract
We report a patient with a unique and complex cytogenetic abnormality involving mosaicism for a small ring X and deleted Xp derivative chromosome with tandem duplication at the break point. The patient presented with failure to thrive, muscular hypotonia, and minor facial anatomic anomalies, all concerning for Turner syndrome. Brain MRI revealed mild thinning of the corpus callosum, an apparent decrease in ventricular white matter volume, and an asymmetric myelination pattern. Array comparative genome hybridization analysis revealed mosaicism for the X chromosome, deletion of the short arm of an X chromosome, and a duplication of chromosome region Xp11.21-p11.22. G-banded chromosome and FISH analyses revealed three abnormal cell lines: 46,X,der(X)del(X)(p11.23)dup(X)(p11.21p11.22)/46,X,r(X)(q11.1q13.1)/45,X. The small ring X chromosome was estimated to be 5.2 Mb in size and encompassed the centromere and Xq pericentromeric region. X chromosome inactivation (XCI) studies demonstrated a skewed pattern suggesting that the ring X remained active, likely contributing to the observed clinical features of brain dysmyelination. We hypothesize that a prezygotic asymmetric crossing over within a loop formed during meiosis in an X chromosome with a paracentric inversion resulted in an intermediate dicentric chromosome. An uneven breakage of the dicentric chromosome in the early postzygotic period might have resulted in the formation of one cell line with the X chromosome carrying a terminal deletion and pericentromeric duplication of the short arm and the second cell line with the X chromosome carrying a complete deletion of Xp. The cell line carrying the deletion of Xp could have then stabilized through self-circularization and formation of the ring X chromosome.
Circuit life span in critically ill children on continuous renal replacement treatment: a prospective observational evaluation study
Authors: del Castillo Jimena , López-Herce Jesús , Cidoncha Elena , Urbano Javier , Mencía Santiago , Santiago Maria J , Bellón Jose M 
Published: July 2008

Abstract
Introduction
One of the greatest problems with continuous renal replacement therapy (CRRT) is early coagulation of the filters. Few studies have monitored circuit function prospectively. The purpose of this study was to determine the variables associated with circuit life in critically ill children with CRRT.

Methods
A prospective observational study was performed in 122 children treated with CRRT in a pediatric intensive care unit from 1996 to 2006. Patient and filter characteristics were analyzed to determine their influence on circuit life. Data were collected on 540 filters in 122 patients and an analysis was performed of the 365 filters (67.6%) that were changed due to circuit coagulation.

Results
The median circuit life was 31 hours (range 1 to 293 hours). A univariate and multivariate logistic regression study was performed to assess the influence of each one of the factors on circuit life span. No significant differences in filter life were found according to age, weight, diagnoses, pump, site of venous access, blood flow rate, ultrafiltration rate, inotropic drug support, or patient outcome. The mean circuit life span was longer when the heparin dose was greater than 20 U/kg per hour (39 versus 29.1 hours; P = 0.008), with hemodiafiltration compared with hemofiltration (34 versus 22.7 hours; P = 0.001), with filters with surface areas of 0.4 to 0.9 m2 (38.2 versus 26.1 hours; P = 0.01), and with a catheter size of 6.5 French or greater (33.0 versus 25.0 hours; P = 0.04). In the multivariate analysis, hemodiafiltration, heparin dose of greater than 20 U/kg per hour, filter surface area of 0.4 m2 or greater, and initial creatinine of less than 2 mg/dL were associated with a filter life of more than 24 and 48 hours. Total effluent rate of greater than 35 mL/kg per hour was associated only with a filter life of more than 24 hours.

Conclusion
Circuit life span in CRRT in children is short but may be increased by the use of hemodiafiltration, higher heparin doses, and filters with a high surface area.
MeV+R: using MeV as a graphical user interface for Bioconductor applications in microarray analysis
Authors: Chu Vu T , Gottardo Raphael , Raftery Adrian E , Bumgarner Roger E , Yeung Ka Yee 
Published: July 2008

AbstractWe present MeV+R, an integration of the JAVA MultiExperiment Viewer program with Bioconductor packages. This integration of MultiExperiment Viewer and R is easily extensible to other R packages and provides users with point and click access to traditionally command line driven tools written in R. We demonstrate the ability to use MultiExperiment Viewer as a graphical user interface for Bioconductor applications in microarray data analysis by incorporating three Bioconductor packages, RAMA, BRIDGE and iterativeBMA.
The selective nonpeptide CXCR2 antagonist SB225002 ameliorates acute experimental colitis in mice
Authors: Bento Allisson Freire , Leite Daniela Ferraz Pereira , Claudino Rafaela Franco , Hara Daniela Balz , Leal Paulo César , Calixto João B 
Published: July 2008

AbstractAlthough neutrophils are strongly implicated in eliminating pathogens, excessive recruitment may cause tissue damage. Therefore, reducing cell influx during an inflammatory process may be a potential target for treating inflammatory bowel diseases (IBD). As CXCR2 is involved in neutrophil migration, this study aimed to evaluate whether the systemic therapeutic treatment with selective CXCR2 antagonist SB225002 ameliorates experimental colitis, which was induced in mice by 2,4,6-trinitrobenzene sulfonic acid (TNBS). After colitis establishment (24 h), mice were treated with SB225002. At later time-points, up to 72 h, mice were monitored for body weight loss and overall mortality. At the time of sacrifice, colonic tissues were scored for macro- and microscopic damage, and cytokine levels, myeloperoxidase (MPO) activity, and protein expression were analyzed. TNBS administration induced macro- and microscopic damage in colon tissue, leading in most cases to animal death. Curative treatment with SB225002 significantly reduced all of the parameters analyzed, leading to an improvement of inflammatory signs. SB225002 reduced neutrophil influx, MPO activity, IL-1β, MIP-2, and keratinocyte-derived chemokine (KC) levels and the expression of vascular endothelial growth factor, inducible NO synthase, and cyclooxygenase-2 proteins into the colon tissue. Levels of IL-4 and IL-10 were increased significantly in the colons of animals treated with SB225002. Additionally, curative treatment with mouse anti-KC significantly reduced MPO activity and colonic damage. These results taken together demonstrate that a selective blockade of CXCR2 consistently reduced TNBS-induced colitis, suggesting that the use of SB225002 is a potential therapeutic approach for the treatment of IBD and other related inflammatory disorders.
The Mammalian Target of Rapamycin Complex 1 Regulates Leptin Biosynthesis in Adipocytes at the Level of Translation: The Role of the 5′-Untranslated Region in the Expression of Leptin Messenger Ribonucleic Acid
Authors: Chakrabarti Partha , Anno Takatoshi , Manning Brendan D. , Luo Zhijun , Kandror Konstantin V. 
Published: July 2008

Abstract
Leptin production by adipose cells in vivo is increased after feeding and decreased by food deprivation. However, molecular mechanisms that control leptin expression in response to food intake remain unknown. Here, we test the hypothesis that leptin expression in adipose cells is regulated by nutrient- and insulin-sensitive mammalian target of rapamycin complex 1 (mTORC1)-mediated pathway. The activity of mTORC1 in 3T3-L1 adipocytes was up-regulated by stable expression of either constitutively active Rheb or dominant-negative AMP-activated protein kinase. In both cases, expression of endogenous leptin was significantly elevated at the level of translation. To investigate the role of leptin 5′-untranslated region (UTR) in the regulation of protein expression, we created bicistronic reporter constructs with and without the 5′-UTR. We found that the presence of leptin 5′-UTR renders mRNA resistant to regulation by mTORC1. It appears, therefore, that mTORC1 controls translation of leptin mRNA via a novel mechanism that does not require the presence of either the 5′-terminal oligopyrimidine tract or the 5′-UTR.
Quantitative Analysis of Circulating Methylated DNA as a Biomarker for Hepatocellular Carcinoma
Authors: Chan K C Allen , Lai Paul B S , Mok Tony S K , Chan Henry L Y , Ding Chunming , Yeung S W , Lo Y M Dennis 
Published: July 2008

Abstract
Background: Hypermethylation of the RASSF1A [Ras association (RalGDS/AF-6) domain family member 1A] gene is frequently observed in hepatocellular carcinoma (HCC). We evaluated the analysis of circulating hypermethylated RASSF1A for detecting HCC and assessing its prognosis.
Methods: In module 1, we studied 63 pairs of HCC patients and age- and sex-matched chronic hepatitis B virus (HBV) carriers, as well as 50 healthy volunteers. In module 2, we studied 22 HCC patients with cancer detected through a surveillance program. The concentrations of circulating hypermethylated RASSF1A sequences were measured by real-time PCR after digestion with a methylation-sensitive restriction enzyme.
Results: We detected hypermethylated RASSF1A sequences in the sera of 93% of HCC patients, 58% of HBV carriers, and 8% of the healthy volunteers. The median RASSF1A concentrations for the HCC patients and HBV carriers were 7.70 × 105 copies/L and 1.18 × 105 copies/L, respectively (P &lt; 0.01). The detection of low concentrations in HBV carriers is consistent with previous findings that RASSF1A hypermethylation is an early event in HCC pathogenesis and can be found in premalignant liver tissues. Use of a marker cutoff value of 1 × 106 copies/L also identifies 50% of α-fetoprotein-negative HCC cases. Patients with higher RASSF1A concentrations at diagnosis or 1 year after tumor resection showed poorer disease-free survival (P &lt; 0.01). For the HBV carriers who underwent HCC surveillance and subsequently developed HCC, the circulating concentration of RASSF1A increased significantly from the time of enrollment to cancer diagnosis (P = 0.014).
Conclusions: Detection and quantification of circulating methylated RASSF1A sequences are useful for HCC screening, detection, and prognostication.
Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases
Authors: Kraenzlin Marius E , Kraenzlin Claude A , Meier Christian , Giunta Cecilia , Steinmann Beat 
Published: July 2008

AbstractBackground: The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults.Methods: We used a commercially available reagent set (Chromsystems Instruments & Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10).Results: The mean intraassay and interassay CVs were &lt;6% and &lt;8% for both PYD and DPD, respectively. The reference interval was constant for premenopausal women in the age group 20–49 years. In men, cross-link values peaked at 20–29 years and decreased thereafter. Women with postmenopausal osteoporosis had significantly higher PYD (51%) and DPD (58%) values compared to premenopausal women. Similar results were found in osteoporotic men. In children the highest values were found in the first weeks and months after birth, followed by a decrease of 50%–60% at age 11–14 years. In metabolic bone diseases cross-link concentrations were significantly increased. The DPD:PYD ratio (mean value approximately 0.2) was remarkably constant in all populations evaluated.Conclusions: The automated HPLC assay is a precise and convenient method for PYD and DPD measurement. We established reference intervals for adult women and men and for children up to 14 years old. The cross-link concentrations we determined by use of this HPLC method confirm its clinical value in enabling identification of increased bone resorption in patients with metabolic bone diseases.
The Assessment of Dangerous Behaviour: Research and Clinical Issues
Authors: Ward Tony , Eccleston Lynne 
Published: July 2008

AbstractMental health professionals in forensic settings are increasingly called upon to assess the probability of dangerous behaviour, or level of risk, that certain individuals pose to the community. These assessments may inform decisions concerning the containment and management of violent offenders within forensic settings, sentencing options, and whether or not to grant bail or parole. In this paper we provide an overview of the key issues associated with the assessment of dangerous behaviour. Specifically, we briefly consider the debate on clinical versus actuarial assessments of dangerous behaviour and risk, and provide a synopsis of the current research on dangerous behaviour, focusing on the key areas of mental disorder, psychopathy, and substance use. Finally, we outline guidelines for addressing specific content areas in the clinical risk-assessment process and discuss areas for future research.
Conduct Problems Across Home and School
Authors: Little Emma , Hudson Alan , Wilks Ray 
Published: July 2008

AbstractMany children are reported by parents to exhibit problems of conduct at home, and similarly many children are reported by teachers to exhibit conduct problems at school. Surprisingly, very little research has examined the extent to which children exhibit behavioural problems in the clinical range at both home and school. The current study examined the co-occurrence of problems at home and school in a sample of 189 children in schools in Victoria. The total sample consisted of (a) 124 children whose parents responded to invitations to participate distributed through schools, and (b) 65 children who had been referred to various agencies because of conduct problems. The occurrence of problems at home was assessed using the Child Behaviour Checklist (Achenbach & Edelbrock, 1983), and problems at school were assessed using the Teacher Report Form (Achenbach & Edelbrock, 1986). For the school sample, 7 children (5.6%) exhibited conduct problems in both settings (scores in the borderline/ clinical range), with 27 children (21.7%) exhibiting them in one setting only. For the clinic sample, 39 children (60.0%) exhibited problems in both settings, with 21 children (32.3%) exhibiting them in one setting only. The results of this study indicate the need for interventions that can be used consistently across home and school settings, as well as independently in single settings when warranted.
The Prevention of Depression in Children: A Pilot Study
Authors: Hannan Anthony P. , Rapee Ronald M. , Hudson Jennifer L. 
Published: July 2008

AbstractTwenty, fifth- and sixth-grade students who reported mild depressive symptomatology were placed into a brief intervention program. The program was conducted over eight, weekly, 90-minute sessions and aimed to teach students depression management strategies and positive coping skills. Significant reductions in symptoms of depression, anxiety and externalising behaviour were found and maintained for six months. The results lend support to the effectiveness of this brief program for early intervention in depression. The potential for the program to prevent the later development of clinical depression and related problems is discussed.
A Brief Cognitive-Behavioural Intervention for a 10-year-old Child With an Adjustment Disorder — A Case Study
Authors: Allen Kathleen 
Published: July 2008

AbstractThis case study reports a brief cognitive-behavioural intervention with a 10-year-old child who presented in a clinical setting with an adjustment disorder which has been rarely discussed in the literature. The focus of the paper is on the intervention which was individually devised to suit the child, the particular presenting problems, and the family context of the child. Although there were no quantitative data collected, the outcome as reported by the client and her carer, together with clear behavioural indicators, attested to positive effects of therapy. Relevant factors that contributed to the outcome are discussed.
Constant Versus Rhythmic Muscle Tension in Applied Tension
Authors: Bodycoat Nicole , Grauaug Lisa , Olson Andrew , Page Andrew C. 
Published: July 2008

AbstractThe research aimed to examine ways in which applied tension for blood-injury phobia may be improved. The effect on blood pressure of two forms of muscle tension under three different rates of breathing is reported. Forty-five participants were randomly assigned to use either constant or rhythmic muscle tension under three different breathing rates (i.e., fast, slow, and normal). The effects on systolic and diastolic blood pressure were measured. Results indicated that both constant and rhythmic forms of muscle tension increased systolic and diastolic blood pressure. However, rhythmic tension was significantly more effective than constant tension at raising diastolic blood pressure. Breathing rate had no differential effects on blood pressure. Suggestions are made about modifications of Applied Tension in the treatment of blood-injury phobia.