bluuebunny/crossref_metadata_2025_split huggingface dataset:
GPI‐anchored CEA family glycoproteins CEA and CEACAM6 mediate their biological effects through enhanced integrin α5β1‐fibronectin interaction&
Authors: Ordonez Cosme , Zhai Alexander B. , Camacho‐Leal Pilar , Demarte Luisa , Fan Mannie M.Y. , Stanners Clifford P. 
Published: December 2006

AbstractCarcinoembryonic antigen (CEA) and CEA family member CEACAM6 are glycophosphatidyl inositol (GPI)‐anchored, intercellular adhesion molecules that are up‐regulated in a wide variety of human cancers, including colon, breast, and lung. When over‐expressed in a number of cellular systems, these molecules are capable of inhibiting cellular differentiation and anoikis, as well as disrupting cell polarization and tissue architecture, thus increasing tumorigenicity. The present study shows that perturbation of the major fibronectin receptor, integrin α5β1, underlies some of these biological effects. Using confocal microscopy and specific antibodies, CEA and CEACAM6 were demonstrated to co‐cluster with integrin α5β1 on the cell surface. The presence of CEA and CEACAM6 was shown to lead to an increase in the binding of the integrin α5β1 receptor to its ligand fibronectin, without changing its cell surface levels, resulting in increased adhesion of CEA/CEACAM6‐expressing cells to fibronectin. More tenacious binding of free fibronectin to cells led to enhanced fibronectin matrix assembly and the formation of a polymerized fibronectin “cocoon” around the cells. Disruption of this process with specific monoclonal antibodies against either fibronectin or integrin α5β1 led to the restoration of cellular differentiation and anoikis in CEA/CEACAM6 producing cells. J. Cell. Physiol. 210: 757–765, 2007. © 2006 Wiley‐Liss, Inc.
Low‐intensity pulsed ultrasound (LIPUS) induces RANKL, MCP‐1, and MIP‐1β expression in osteoblasts through the angiotensin II type 1 receptor
Authors: Bandow Kenjiro , Nishikawa Yoshiaki , Ohnishi Tomokazu , Kakimoto Kyoko , Soejima Kazuhisa , Iwabuchi Sadahiro , Kuroe Kazuto , Matsuguchi Tetsuya 
Published: December 2006

AbstractConstant mechanical stress is essential for the maintenance of bone mass and strength, which is achieved through the cooperative functions of osteoblasts and osteoclasts. However, it has not been fully elucidated how these cell types mediate mechanical signals. Low‐intensity pulsed ultrasound (LIPUS) therapy is a recently developed method for application of mechanical stress, and is used clinically to promote bone fracture healing. In the present study, we applied LIPUS to osteoblasts at different stages of maturation and analyzed their chemokine and cytokine expression. In comparison with their immature counterparts, mature osteoblasts expressed significantly higher levels of mRNAs for the receptor activator of nuclear factor kappa B ligand (RANKL), monocyte chemoattractant protein (MCP)‐1, and macrophage‐inflammatory protein (MIP)‐1β after a few hours of LIPUS treatment. Intriguingly, protein and mRNA expression of angiotensin II type 1 receptor (AT1), a known mechanoreceptor in cardiomyocytes, was detected in osteoblasts, and the level of expression increased significantly during cell maturation. Furthermore, LIPUS‐induced extracellular signal‐regulated kinase (ERK) phosphorylation and RANKL/chemokine expression was abrogated by a specific AT1 inhibitor. Thus, AT1 may play one of the essential roles in bone metabolism as a mechanoreceptor of osteoblasts. J. Cell. Physiol. 211: 392–398, 2007. © 2006 Wiley‐Liss, Inc.
Effects of OPA1 mutations on mitochondrial morphology and apoptosis: Relevance to ADOA pathogenesis
Authors: Olichon Aurélien , Landes Thomas , Arnauné‐Pelloquin Laetitia , Emorine Laurent J. , Mils Valérie , Guichet Agnès , Delettre Cécile , Hamel Christian , Amati‐Bonneau Patrizia , Bonneau Dominique , Reynier Pascal , Lenaers Guy , Belenguer Pascale 
Published: December 2006

AbstractTo characterize the molecular links between type‐1 autosomal dominant optic atrophy (ADOA) and OPA1 dysfunctions, the effects of pathogenic alleles of this dynamin on mitochondrial morphology and apoptosis were analyzed, either in fibroblasts from affected individuals, or in HeLa cells transfected with similar mutants. The alleles were missense substitutions in the GTPase domain (OPA1G300E and OPA1R290Q) or deletion of the GTPase effector domain (OPA1Δ58). Fragmentation of mitochondria and apoptosis increased in OPA1R290Q fibroblasts and in OPA1G300E transfected HeLa cells. OPA1Δ58 did not influence mitochondrial morphology, but increased the sensitivity to staurosporine of fibroblasts. In these cells, the amount of OPA1 protein was half of that in control fibroblasts. We conclude that GTPase mutants exert a dominant negative effect by competing with wild‐type alleles to integrate into fusion‐competent complexes, whereas C‐terminal truncated alleles act by haplo‐insufficiency. We present a model where antagonistic fusion and fission forces maintain the mitochondrial network, within morphological limits that are compatible with cellular functions. In the retinal ganglion cells (RGCs) of patients suffering from type‐1 ADOA, OPA1‐driven fusion cannot adequately oppose fission, thereby rendering them more sensitive to apoptotic stimuli and eventually leading to optic nerve degeneration. J. Cell. Physiol. 211: 423–430, 2007. © 2006 Wiley‐Liss, Inc.
p66Shc is involved in promoting HIF‐1α accumulation and cell death in hypoxic T cells
Authors: Carraro Fabio , Pucci Annalisa , Pellegrini Michela , Giuseppe Pelicci Pier , Baldari Cosima T. , Naldini Antonella 
Published: December 2006

AbstractHypoxia results in adaptationally appropriate alterations of gene expression through the activation of hypoxia‐inducible factor (HIF)‐1 to overcome any shortage of oxygen. Peripheral blood mononuclear cells may be exposed to low oxygen tensions for different times as they migrate between blood and various tissues. We and others have previously shown that T‐cell adaptation to hypoxia is characterized by a modulation of cytokine expression and an inhibition of T‐cell activation. We have recently demonstrated that the adaptor protein p66Shc negatively regulates T‐cell activation and survival. We here show that hypoxia enhances HIF‐1α accumulation and vascular endothelial growth factor production in T cells. Hypoxic T cells expressed high levels of p21WAF1/CIP1, of the pro‐apoptotic molecules BNIP3, a classic HIF target gene, and BAX, as well as low levels of the anti‐apoptotic molecule BCLxl, associated with an induction of cell death. We found out that hypoxic T cells expressed p66Shc. Furthermore, using T‐cell transfectants expressing p66Shc, as well as T cells derived from mice p66Shc−/−, we defined a role of p66Shc in T‐cell responses to hypoxia. Of interest, hypoxic p66Shc‐positive transfectants expressed higher level of HIF‐1α than negative controls. Thus, p66Shc may play an important role in downstream hypoxic signaling, involving HIF‐1α protein accumulation and cell death in T lymphocytes. J. Cell. Physiol. 211: 439–447, 2007. © 2006 Wiley‐Liss, Inc.
Endothelial cell response to lactate: Implication of PAR modification of VEGF
Authors: Kumar V.B. Sameer , Viji R.I. , Kiran M.S. , Sudhakaran P.R. 
Published: December 2006

AbstractAngiogenesis, the process of formation of new blood vessels from pre‐existing one, occurs in many physiological and pathological conditions, most of which are underlined by hypoxia and resultant accumulation of lactate. Although lactate is known to induce angiogenesis, the mechanism of its action on endothelial cells (ECs) is not known. The present study was designed to examine the response of ECs to lactate. Morphological analysis revealed that human umbilical vein endothelial cells (HUVECs) in culture respond to lactate by switching over to angiogenic phenotype concomitant with upregulation of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor‐2 (VEGFR2) as determined by reverse transcription‐PCR (RT‐PCR). Apart from increase in the levels of VEGF protein as determined by ELISA, chorio allantoic membrane (CAM) assay using the cell extracts revealed that lactate also increased the angiogenic potency of VEGF. Isolated VEGF, when blotted and subsequently probed with anti‐PAR antibody, revealed considerable reduction in poly‐adenosyl ribosylation of VEGF associated with a significant decrease in the levels of NAD+, in presence of lactate. Thus it appears that ECs respond to lactate by increasing the production of VEGF and modulating its angiogenic potency through poly‐ADP ribosylation (PAR)‐dependent mechanism and thereby switch over to angiogenic phenotype. J. Cell. Physiol. 211: 477–485, 2007. © 2006 Wiley‐Liss, Inc.
List multicolorings of graphs with measurable sets
Authors: Hilton A. J. W. , Johnson P. D. 
Published: December 2006

AbstractIn an ordinary list multicoloring of a graph, the vertices are “colored” with subsets of pre‐assigned finite sets (called “lists”) in such a way that adjacent vertices are colored with disjoint sets. Here we consider the analog of such colorings in which the lists are measurable sets from an arbitrary atomless, semifinite measure space, and the color sets are to have prescribed measures rather than prescribed cardinalities. We adapt a proof technique of Bollobás and Varopoulos to prove an analog of one of the major theorems about ordinary list multicolorings, a generalization and extension of Hall's marriage theorem, due to Cropper, Gyárfás, and Lehel. © 2006 Wiley Periodicals, Inc. J Graph Theory 54: 179–193, 2007
The deglaciation of Clyde Inlet, northeastern Baffin Island, Arctic Canada
Authors: Briner Jason P. , Overeem Irina , Miller Gifford , Finkel Robert 
Published: December 2006

AbstractThe behaviour of ice sheets as they retreated from their Last Glacial Maximum (LGM) positions provides insights into Lateglacial and early Holocene ice‐sheet dynamics and climate change. The pattern of deglaciation of the Laurentide Ice Sheet (LIS) in arctic fiord landscapes can now be well dated using cosmogenic exposure dating. We use cosmogenic exposure and radiocarbon ages to constrain the deglaciation history of Clyde Inlet, a 120 km long fiord on northeastern Baffin Island. The LIS reached the continental shelf during the LGM, retreated from the coastal lowlands by 12.5 ± 0.7 ka (n = 3), and from the fiord mouth by 11.7 ± 2.2 ka (n = 4). Rapid retreat from the outer fiord occurred 10.3 ± 1.3 ka (n = 6), with the terminus reaching the inner fiord shortly after 9.4 ka (n = 2), where several moraine systems were deposited between ca. 9.4 and ca. 8.4 ka. These moraines represent fluctuations of the LIS during the warmest summers since the last interglaciation, and this suggests that the ice sheet was responding to increased snowfall. Before retreating from the head of Clyde Inlet, the LIS margin fluctuated at least twice between ca. 7.9 and ca. 8.5 ka, possibly in response to the 8.2 ka cold event. Copyright © 2006 John Wiley & Sons, Ltd.
Using geochemistry as a tool for correlating proximal andesitic tephra: case studies from Mt Rainier (USA) and Mt Ruapehu (New Zealand)
Authors: Donoghue Susan L. , Vallance James , Smith Ian E. M. , Stewart Robert B. 
Published: December 2006

AbstractVolcanic hazards assessments at andesite stratovolcanoes rely on the assessment of frequency and magnitude of past events. The identification and correlation of proximal and distal andesitic tephra, which record the explosive eruptive history, are integral to such assessments. These tephra are potentially valuable stratigraphic marker beds useful to the temporal correlation and age dating of Quaternary volcanic, volcaniclastic and epiclastic sedimentary deposits with which they are interbedded. At Mt Ruapehu (New Zealand) and Mt Rainier (USA), much of the detail of the recent volcanic record remains unresolved because of the difficulty in identifying proximal tephra. This study investigates the value of geochemical methods in discriminating andesitic tephra. Our dataset comprises petrological and geochemical analyses of tephra that span the late Quaternary eruptive record of each volcano. Our data illustrate that andesitic tephra are remarkably heterogeneous in composition. Tephra compositions fluctuate widely over short time intervals, and there are no simple or systematic temporal trends in geochemistry within either eruptive record. This complexity in tephra geochemistry limits the application of geochemical approaches to tephrostratigraphic studies, beyond a general characterisation useful to provenance assignation. Petrological and geochemical data suggest that the products of andesite systems are inherently variable and therefore intractable to discrimination by simple geochemical methods alone. Copyright © 2006 John Wiley & Sons, Ltd.
Synthetic lubricants based on sebacic complex esters
Authors: Mirci L. E. , Boran S. , Pode V. , Resiga D. 
Published: December 2006

AbstractThis paper presents the results of a study carried out in order to produce synthetic ester oils of a mixed structure based on sebacic acid with beneficial tribological properties. These series of unsymmetrical diesters were synthesized using superior aliphatic alcohols such as 2‐ethyl‐hexanol, isodecanol and isotridecanol, along with special alcohols of a complex alkyl‐aryl structure, namely 2‐phenoxy‐ethanol, 2‐[(o‐sec butyl) phenoxy] ethanol and 2‐[(p‐nonyl) phenoxy] ethanol. Also synthesized were symmetric esters based on the complex aliphatic‐aromatic alcohols with pendant aliphatic chain. The variation in the principal properties as a function of both the length of the aliphatic chain introduced by the aliphatic alcohol, and the length and position of the aliphatic pendant chain on the aromatic nucleus of the complex alkyl‐aryl alcohol was assessed. Based on this, it has been possible to obtain fluid products with variable properties. The introduction of the aromatic function in the structure of these diesters led to interesting features, and the superior values recorded for the main tribological characteristics demonstrated their versatility. Copyright © 2006 John Wiley & Sons, Ltd.
Separation of oligonucleotides in N‐methyl‐formamide‐based polymer matrices by capillary electrophoresis
Authors: Sjödahl Johan , Lindberg Peter , Roeraade Johan 
Published: December 2006

AbstractN‐Methylformamide (NMF)‐based matrices for capillary electrophoretic separation of nucleic acids have been developed. The use of an organic solvent as liquid base for the separation matrices allowed a hydrophobic polymer, C16‐derivatized 2‐hydroxyethyl cellulose (HEC), to be employed as structural element in the sieving medium. With a matrix consisting of 5% w/v of this polymer dissolved in NMF containing 50 mM ammonium acetate, p(dA)12–18 and p(dA)40–60 oligonucleotides were baseline separated. The addition of ammonium acetate to the buffer and separation matrix resulted in enhanced separation efficiency. Furthermore, it was possible to tailor the sieving performance of the separation medium by the use of a binary mixture of C16‐derivatized HEC and PVP. Differences in sieving behavior of the various matrices evaluated are discussed.
DFG‐research‐group: Modelling reinforcement corrosion – overview of the project
Authors: Schießl P. , Osterminski K. 
Published: December 2006

AbstractA strong interest for the durability of reinforced concrete structures currently exists in industry and research [1]. Against the background of immense costs for maintaining reinforced concrete structures and repairing damage caused by corroding reinforcement steel, this interest lead to a German joint research project. The aim of this network‐based (www.bam.de/dfg537.htm) research group is, to deliver the basic knowledge of the corrosion propagation and to make a probabilistic tool available for engineers so that a complete design for durability, concerning reinforcement corrosion, will be possible.
Immersed electrokinetic finite element method
Authors: Liu Yaling , Liu Wing Kam , Belytschko Ted , Patankar Neelesh , To Albert C. , Kopacz Adrian , Chung Jae‐Hyun 
Published: December 2006

AbstractA new method is proposed for modelling the electrokinetic‐induced mechanical motion of particles in a fluid domain under an applied electric field. In this method, independent solid meshes move in a fixed background field mesh that models the fluid and the electric field. This simple strategy removes the need for expensive mesh updates. Furthermore, the reproducing kernel particle functions enable efficient coupling of various immersed deformable solids with the surrounding viscous fluid in the presence of an applied electric field. The electric force on a particle is calculated by the Maxwell stress tensor method. For the first time, three‐dimensional assembly of nano/biomaterials of various geometries and electrical properties have been comprehensively studied using the new method. Simulation of the dynamic process of electro‐manipulation of individual and multiple cells agrees well with experimental data. Preliminary results for selective deposition of viruses and stretching of a DNA molecule are also presented. Copyright © 2006 John Wiley & Sons, Ltd.
The international volunteering market: market segments and competitive relations
Authors: Dolnicar Sara , Randle Melanie 
Published: December 2006

Abstract

The number of nonprofit and social agencies relying on the help of volunteers has grown enormously in recent decades. This has lead to increased competition between these organisations for the limited resources available, and the growing adoption of what have traditionally been considered ‘commercial’ business techniques such as marketing. There have been calls for greater and more sophisticated use of ‘tried and tested’ marketing concepts such as competition, segmentation and positioning to help volunteering organisations manage this pressure effectively. This study shines the spotlight on individuals who volunteer for multiple types of organisations in an effort to determine which organisations are competing for the same volunteers. More specifically:
Factor analyses are computed and four segments of volunteers are identified: ‘altruists’, ‘leisure volunteers’, ‘political volunteers’ and ‘church volunteers’;
Positioning maps are constructed to illustrate the proximity of each organisation type in relation to key competitors; and
Detailed profiles are provided for each segment to provide insight into the nature of the groupings.

Copyright © 2006 John Wiley & Sons, Ltd.
Use of office discipline referrals in school‐wide decision making: A practical example
Authors: Clonan Sheila M. , McDougal James L. , Clark Karrie , Davison Sigrid 
Published: December 2006

AbstractProblem‐solving approaches incorporating interventions at multiple levels have gained in popularity in recent years. One such model, Positive Behavioral Interventions and Supports (PBIS), was developed to assist schools to more effectively promote positive student behavior through a systematic and recursive approach to developing and assessing school‐wide behavioral interventions. The focus of this article is on how data such as office discipline referrals (ODRs) can be used to inform decisions made by school problem‐solving teams. Following a brief overview of the PBIS model, specific recommendations are offered for school psychologists related to the collection and use of ODRs for intervention design, revision, and evaluation. In addition, preliminary data from a case‐study implementation are reviewed, highlighting the use of a data‐based decision‐making model for promoting positive student behavior. © 2007 Wiley Periodicals, Inc. Psychol Schs 44: 19–27, 2007.
Implementing school‐wide behavior change: Lessons from the field
Authors: George Michael P. , White George P. , Schlaffer Joseph J. 
Published: December 2006

AbstractIn this article, we examine two schools that successfully adopted school‐wide positive behavior interventions and highlight some of the common features that contributed to their success. As part of our analysis, we draw upon the theoretical literature on organizational change to discuss factors that supported these successful school‐wide reform efforts, including the contributions of administrators, teachers, and school psychologists. © 2007 Wiley Periodicals, Inc. Psychol Schs 44: 41–51, 2007.
Antecedent strategies to promote appropriate classroom behavior
Authors: Kern Lee , Clemens Nathan H. 
Published: December 2006

AbstractIn response to ongoing concerns with student academic and behavior problems, antecedent strategies have garnered increasing attention. Antecedent intervention approaches focus on structuring the environment to prevent problems and enhance motivation. At the class‐wide level, implementation of these strategies can create a structured and orderly environment to which most students are responsive. In the case of persistent behavior problems, specific events that precede problem behavior can be removed or modified to create individualized antecedent interventions. The empirical literature base supporting the value of this approach has witnessed rapid growth. In this article, we offer a rationale for the use of antecedent strategies and provide literature‐based examples of applications within school settings at both the class‐wide and individual levels. In addition, we discuss practical considerations for implementing antecedent interventions. © 2007 Wiley Periodicals, Inc. Psychol Schs 44: 65–75, 2007.
Proteomic and transcriptomic analyses of differential stress/inflammatory responses in mandibular lymph nodes and oropharyngeal tonsils of European wild boars naturally infected with Mycobacterium bovis
Authors: Naranjo Victoria , Villar Margarita , Martín‐Hernando Ma Paz , Vidal Dolors , Höfle Ursula , Gortazar Christian , Kocan Katherine M. , Vázquez Jesús , de la Fuente José 
Published: December 2006

AbstractDifferential stress/inflammatory responses were characterized at the mRNA and protein levels in mandibular lymph nodes (MLN) and oropharyngeal tonsils of European wild boars (Sus scrofa), naturally infected with Mycobacterium bovis. Suppression‐subtractive hybridization combined with immunohistochemistry and/or quantitative real‐time RT‐PCR were used to identify and characterize abundant stress/inflammatory gene sequences differentially expressed in tuberculous (TB+) wild boars. Genes identified in MLN and tonsils corresponded to serum amyloid A, arginase I, osteopontin, lysozyme, annexin I, and heat shock proteins, respectively. Global protein patterns in MLN and tonsils were compared between TB+ and nontuberculous (TB−) boars by 2‐DE and MALDI‐TOF MS. Five proteins, including stress/inflammatory proteins annexin V, serum albumin, and apolipoprotein A1 were found at lower levels in MLN of TB+ boars. Manganese superoxide dismutase was found up‐regulated in MLN of TB+ boars. Five proteins, including creatine kinase and MHC class II antigens were found up‐regulated in tonsils of TB+ boars. These results demonstrated differential stress/inflammatory responses in wild boars naturally infected with M. bovis and suggest possible markers of tuberculosis in this species that may prove useful for future studies of host–pathogen interactions and for diagnostics and vaccine development.