bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tieg3/Klf11 induces apoptosis in OLI‐neu cells and enhances the TGF‐β signaling pathway by transcriptional repression of Smad7
Authors: Gohla Gabriele , Krieglstein Kerstin , Spittau Björn 
Published: January 2008

AbstractTGF‐β signaling is indispensible for development of the nervous system since it regulates ontogenetic cell death. The recently identified TGF‐β‐inducible zinc finger protein Tieg3/Klf11 belongs to the family of Sp1/Klf‐like transcription factors and shares all structural and functional features with other Tieg proteins. Using the established TGF‐β‐responsive oligodendroglial cell line OLI‐neu, we analyzed the role of Tieg3/Klf11 in TGF‐β signaling. In this report, we show that Tieg3/Klf 11 mimics TGF‐β effects by inducing apoptotic cell death accompanied by activation of caspase‐3. Moreover, we demonstrate that Tieg3/Klf11 enhances TGF‐β signaling by transcriptional repression of the inhibitory Smad7 and, thereby, disrupts the negative feedback loop of the TGF‐β signaling pathway. Loss of the N‐terminal repression domains of Tieg3/Klf11 abrogates the pro‐apoptotic nature of this transcription factor and abolishes the enhancement of Smad‐mediated TGF‐β responses. In conclusion, we provide evidence that the recently identified transcription factor Tieg3/Klf11 is a downstream mediator of TGF‐β‐induced apoptosis in the oligodendroglial cell line OLI‐neu. Since other signaling molecules are able to initiate transcription of members of the Tieg family, the ability of Tieg3/Klf11 to modulate TGF‐β signaling by transcriptional inhibition of Smad7 might be an important clue for the understanding of the crosstalk between different signaling pathways. J. Cell. Biochem. 104: 850–861, 2008. © 2008 Wiley‐Liss, Inc.
Perceptions of coercion in the questioning of criminal suspects
Authors: Moston Stephen , Fisher Megan 
Published: January 2008

AbstractDespite the popular perception that criminal investigations are often solved through the collection of objective or scientific evidence, most cases submitted for prosecution rely on confessions by suspects. Surprisingly, very little is known about which techniques are effective at eliciting admissions from guilty suspects, and in turn, which might be considered to be coercive. In the current study, 200 mock jurors (in Australia) were asked to rate the acceptability of 13 common police interviewing techniques (such as pointing out contradictions, minimisation, silence, etc.). Subjects read one of four versions of a transcript of an interrogation with a suspected sex offender. The transcripts only differed in one detail, the final outcome of the interrogation, with each subject reading one of four different endings (suspect confessed, made a partial admission, denied, or used their right to silence). The results showed that the majority of the strategies were perceived to be coercive, but acceptable. Further, the ‘ends do not justify the means’, in that perceptions of coercion were consistent regardless of the outcome of the interview. The findings are discussed in relation to existing laws and procedures governing police interrogation of suspects, including the ambiguities over the definitions of coercion, oppression, and torture. Copyright © 2008 John Wiley & Sons, Ltd.
Immortalized human neural progenitor cells from the ventral telencephalon with the potential to differentiate into GABAergic neurons
Authors: Zhang Haiyan , Wang Yisong , Zhao Yongmei , Yin Yanling , Xu Qiuyan , Xu Qunyuan 
Published: January 2008

AbstractHuman neural progenitor cells (hNPCs) are believed to have important potential in clinical applications and basic neuroscience research. In the present study, we created a new immortalized human neural cell line, hSN12W‐TERT, derived from human fetal ventral telencephalon, using IRES‐based retroviral overexpression of human telomerase reverse transcriptase. We showed that after more than 40 passages, hSN12W‐TERT cells possess high telomerase activity, maintain a normal diploid karyotype, and retain the characteristics of hNPCs. Under proliferative conditions, these cells remained undifferentiated, expressing the neural progenitor cell markers nestin, vimentin, and Sox2. The cells were able to differentiate into neurons, astrocytes, and oligodendrocytes after a significant decrease in the level of telomerase following withdrawal of growth factors. The neurons were postmitotic and achieved electrophysiologic competence. Furthermore, we showed that most neurons were GABAergic, especially on differentiation induced by bone morphogenetic protein–2 (BMP2). RT‐PCR analysis also confirmed that hSN12W‐TERT cells expressed mammalian achaete‐scute homolog 1 (Mash1) and Dlx2, genes associated with the development of GABAergic cortical interneurons. BMP2 exposuremay activate a positive‐feedback loop of BMP signaling in hSN12W‐TERT cells. Our data indicated that this hSN12W‐TERT cell line could be a valuable experimental tool with which to study the regulatory roles of intrinsic and extrinsic factors in human neural stem cell biology and that it would be useful in basic research and in research seeking to discover novel drug targets for clinical candidates. © 2008 Wiley‐Liss, Inc.
Effects of neurosteroids on hydrogen peroxide‐ and staurosporine‐induced damage of human neuroblastoma SH‐SY5Y cells
Authors: Leskiewicz M. , Regulska M. , Budziszewska B. , Jantas D. , Jaworska‐Feil L. , Basta‐Kaim A. , Kubera M. , Jagla G. , Nowak W. , Lason W. 
Published: January 2008

AbstractNeurosteroids are important regulators of central nervous system function and may be involved in processes of neuronal cell survival. This study was undertaken to test the effect of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), pregnenolone (PGL), pregnenolone sulfate (PGLS), and allopregnanolone (Allo) on hydrogen peroxide‐ and staurosporine‐induced toxicity in SH‐SY5Y cells. It has been found that DHEAS inhibited the hydrogen peroxide toxicity in a concentration‐dependent manner, whereas DHEA was active only at higher doses. PGL and PGLS showed neuroprotective effects only at the lowest concentration. Allo had no significant effect on hydrogen peroxide‐evoked lactate dehydrogenase release and at the highest concentration aggravated its toxic effects. Next part of this study evaluated neurosteroid effects on staurosporine‐induced apoptosis. DHEAS, DHEA, and PGL significantly antagonized effects of staurosporine on both caspase‐3 activity and mitochondrial membrane potential. PGLS and Allo inhibited the staurosporine‐induced changes in both apoptotic parameters only at the lowest concentration. Antiapoptotic properties of neurosteroids were positively verified by Hoechst staining. Furthermore, as shown by calcein assay, DHEA, DHEAS, and PGL increased viability of staurosporine‐treated cells, and these effects were attenuated by specific inhibitors of phosphatidylinositol 3‐kinase (PI3‐K) and extracellular signal‐regulated protein kinase (ERK)‐mitogen activated protein kinase (MAPK). These data indicate that neurosteroids prevent SH‐SY5Y cell damage related to oxidative processes and activation of mitochondrial apoptotic pathway. Moreover, neuroprotective effects of DHEA, DHEAS seem to depend on PI3‐K and ERK/MAPK signaling pathways. It can be suggested that, at physiological concentrations, all studied neurosteroids participate in the inhibition of neuronal apoptosis, but with various potencies. © 2008 Wiley‐Liss, Inc.
Signal transduction pathways involved in interaction of galactosylceramide/sulfatide‐containing liposomes with cultured oligodendrocytes and requirement for myelin basic protein and glycosphingolipids
Authors: Boggs Joan M. , Gao Wen , Hirahara Yukie 
Published: January 2008

AbstractWe showed previously that the addition to cultured oligodendrocytes (OLs) of multivalent carbohydrate in the form of liposomes containing the two major glycosphingolipids (GSLs) of myelin, galactosylceramide (GalC) and cerebroside sulfate (Sulf), or galactose conjugated to bovine serum albumin caused clustering of GalC on the extracellular surface and myelin basic protein (MBP) on the cytosolic surface. Multivalent carbohydrate also caused depolymerization of actin microfilaments and microtubules, indicating that interaction of the carbohydrate with the OL surface transmits a transmembrane signal to the cytoskeleton. In the present study we show that inhibition of GSL synthesis with fumonisin B1 prevents clustering of MBP in GalC/Sulf‐negative oligodendrocytes, suggesting that GSLs are required for the effect. Because the effects of multivalent carbohydrate resemble those caused by the addition of anti‐GalC/Sulf antibodies to OLs and because GalC and Sulf can interact with each other by trans carbohydrate–carbohydrate interactions across apposed membranes, these results support the conclusion that the OL receptor for GalC/Sulf in liposomes is GalC/Sulf in the OL membrane. Inhibition of MBP expression using MBP siRNA inhibited GalC clustering, suggesting that MBP is required for the effect. We also investigate the signal transduction pathways involved using a number of enzyme inhibitors. These indicated that the Akt and p42/p44 MAPK pathways, Rho GTPases, and GSK‐3β are involved, consistent with their known involvement in regulation of the cytoskeleton. These interactions between GalC/Sulf‐containing liposomes and the OL membrane may mimic interactions between GalC/Sulf‐enriched signaling domains when OL cell membranes or the extracellular surfaces of compact myelin come into contact. © 2008 Wiley‐Liss, Inc.
Spectroscopic study of polyynes obtained by laser ablation in liquids
Authors: Compagnini Giuseppe , Mita Valentina , D'Urso Luisa , Cataliotti Rosario Sergio , Puglisi Orazio 
Published: January 2008

AbstractShort polyyne chains (⩽C8H2) were produced by laser ablation of graphite rods in water. The results are compared with those obtained by ablation in acetonitrile using vibrational and UV absorption spectroscopy. Since silver surfaces are able to stabilize the obtained sp‐hybridized molecules, surface‐enhanced Raman spectra were recorded and are discussed in terms of surface–molecule interactions. The degradation of the produced species are also considered to support the conclusions. Copyright © 2008 John Wiley & Sons, Ltd.
Effect of surface morphology on surface‐enhanced Raman scattering of 4‐aminobenzenethiol adsorbed on gold substrates
Authors: Shin Kuan Soo 
Published: January 2008

AbstractThe substrate‐dependent surface‐enhanced Raman scattering (SERS) of 4‐aminobenzenethiol (4‐ABT) adsorbed on Au surfaces has been investigated. 4‐ABT is one of the very unique adsorbate molecules whose SERS spectral patterns are known to be noticeably dependent on the relative contribution of chemical enhancement mechanism vs electromagnetic enhancement mechanism. The SERS spectral patterns of 4‐ABT adsorbed on gold substrates with various surface morphology have thus been analyzed in terms of the symmetry types of the vibrational modes. Almost invisibly weak b2 type vibrational bands were observed in the SERS spectra of the 4‐ABT adsorbed on Au colloidal sol nanoparticles or commercially available Au micro‐powders because of the weak contribution of the chemical enhancement. However, greatly enhanced b2 vibrational bands were observed in the spectra of the 4‐ABT molecules adsorbed on the synthesized Au(Zn) sponge or the electrochemically roughened Au(ORC) foil caused by the strong contribution of the chemical enhancement mechanism. Copyright © 2008 John Wiley & Sons, Ltd.
Kinetics and mechanism of myristic acid and isopropyl alcohol esterification reaction with homogeneous and heterogeneous catalysts
Authors: Yalçinyuva Tuncer , Deligöz Hüseyin , Boz İsmail , Ali Gürkaynak Mehmet 
Published: January 2008

AbstractThe reaction of myristic acid (MA) and isopropyl alcohol (IPA) was carried out by using both homogeneous and heterogeneous catalysts. For a homogeneously catalyzed system, the experimental data have been interpreted with a second order, using the power‐law kinetic model, and a good agreement between the experimental data and the model has been obtained. In this approach, it was assumed that a protonated carboxylic acid is a possible reaction intermediate. After a mathematical model was proposed, reaction rate constants were computed by the Polymath* program. For a heterogeneously catalyzed system, interestingly, no pore diffusion limitation was detected. The influences of initial molar ratios, catalyst loading and type, temperature, and water amount in the feed have been examined, as well as the effects of catalyst size for heterogeneous catalyst systems. Among used catalysts, p‐toluene sulfonic acid (p‐TSA) gave highest reaction rates. Kinetic parameters such as activation energy and frequency factor were determined from model fitting. Experimental K values were found to be 0.54 and 1.49 at 60°C and 80°C, respectively. Furthermore, activation energy and frequency factor at forward were calculated as 54.2 kJ mol−1 and 1828 L mol−1 s−1, respectively. © 2008 Wiley Periodicals, Inc. 40: 136–144, 2008
Kinetics and mechanism of oxidation of benzohydrazide by bromate catalyzed by vanadium(IV) in aqueous acidic medium
Authors: Shewale S. A. , Phadkule A. N. , Gokavi G. S. 
Published: January 2008

AbstractThe reaction between benzohydrazide and potassium bromate catalyzed by vanadium(IV) was studied under pseudo‐first‐order condition keeping large excess of hydrazide concentration over that of the oxidant. The initiation of the reaction occurs through oxidation of the catalyst vanadium(IV), VO2+, to vanadium(V), VO, which then reacts with hydrazide to give N,N′‐diacylhydrazine and benzoic acid as the products. The order in [H+] is found to be two, and its effect is due to protonation and hydrolysis of oxidized form of the catalyst to form HVO3. The oxidized form of the catalyst, VO, forms a complex with the protonated hydrazide as evidenced by the occurrence of absorption maxima at 390 nm. The rate of the reaction remains unaffected by the increase in the ionic strength. The activation parameters were determined, and data support the mechanism. The detailed mechanism and the rate equation are proposed for the reaction. © 2008 Wiley Periodicals, Inc. Int J Chem Kinet 40: 151–159, 2008
Radiation‐Induced Reaction of Sulfonamide‐Containing Polymers in the Film State for Color Imaging
Authors: Maekawa Yasunari , Kato Jun , Katakai Masashi , Ishihara Masaaki , Enomoto Kazuyuki , Hagiwara Tokio , Ishii Tatsuhito , Itoh Kazuo , Koshikawa Hiroshi , Yoshida Masaru 
Published: January 2008

AbstractA polysulfonamide and copolymers consisting of sulfonamides and amides underwent Fries rearrangement and scission, forming amino groups upon EB irradiation. The EB irradiation of these films with a dose of 500 µC · cm−2, followed by the color forming reaction provided the color imaging of line/space patterns with a resolution of 300 nm.magnified image
The effects of visual and verbal information on attitudes and purchase intentions in internet shopping
Authors: Kim Minjeong , Lennon Sharron 
Published: January 2008

AbstractThe present study investigated how different product presentation formats (visual vs. verbal) influence consumer attitudes toward product and purchase intentions in Internet shopping. The overall results from two Web experiments simulating Internet apparel shopping showed that both visual and verbal information had significant effects on affective and cognitive attitudes toward apparel products, but only verbal information had a significant effect on purchase intention. Though the superiority of visual information was predicted based on prior literature, the results of the study supported verbal superiority. This finding provides an important implication for Internet retailers who tend to pay more attention to visual product presentation. Although visual product presentation is also found to be important, detailed product descriptions are critical to positively influence consumer shopping experience in Internet shopping. © 2008 Wiley Periodicals, Inc.
Deep brain stimulation hardware complications: The role of electrode impedance and current measurements
Authors: Farris Sierra , Vitek Jerrold , Giroux Monique L. 
Published: January 2008

AbstractDeep brain stimulation (DBS) is an effective therapy for advanced Parkinson's disease patients. Successful DBS outcomes depend on appropriate patient selection, surgical placement of the lead, intact hardware systems, optimal programming, and medical management. Despite its importance, there is little guidance in reference to hardware monitoring, hardware troubleshooting, and patient management. Technical manuals produced by the hardware manufacturer (Medtronic, Minneapolis, MN) are not presented in an applied clinical format, making impedance and current measurements difficult to interpret when the results are not straightforward. We present four patients with evolving DBS hardware complications that occurred during long‐term follow‐up, that shaped our clinical protocol for long‐term care management and hardware troubleshooting. © 2007 Movement Disorder Society
Hide‐and‐seek games on a tree to which Eulerian networks are attached
Authors: Alpern Steve 
Published: January 2008

AbstractWe analyze the hide‐and‐seek game Γ(G) on certain networks G. The hider picks a hiding point yin Gand the searcher picks a unit speed path S(t) in G, starting at any point S(0). The payoff in this zero‐sum game is the capture time T = T(S,y) = min{t: S(t) = y}. Such games have been studied before, but mainly with the simplifying assumption that the searcher's starting point S(0) is specified and known to the hider. We call a network partly Eulerian if it consists of a tree (of length aand radius r) to which a finite number of disjoint Eulerian networks (of total length b) are attached, each at a single point. We show that for such networks, a strategy consisting equiprobably of a minimal (Chinese Postman) covering path and its reverse path is optimal for the searcher, while the optimal hider strategy is to assume that the searcher must start at the center of the tree, and to optimize in that (known) game. The value of the game Γ(G) is a+b/2 ‐r. This simplifies and extends a similar result of Dagan and Gal for search games on trees. © 2008 Wiley Periodicals, Inc. NETWORKS, 2008
Examining the relationship between treatment outcomes for academic achievement and social skills in school‐age children with attention‐deficit hyperactivity disorder
Authors: Rutherford Laura E. , Dupaul George J. , Jitendra Asha K. 
Published: January 2008

AbstractThe purpose of this study was to determine the relationship between treatment‐induced changes in academic achievement and social skills in elementary school‐age children with attention‐deficit hyperactivity disorder. A sample of 123 children in grades 1 through 4 with symptoms of inattention, impulsivity and/or hyperactivity, and significant achievement problems in math or reading were identified for participation. Participants were exposed to academic interventions mediated by their teachers, parents, peers, computers, or the student themselves. Data were collected on academic competence using the Woodcock‐Johnson III Test of Achievement and the Academic Competence Evaluation Scales; social skills were assessed using the Social Skills Rating System. Correlations between changes in academic competence and social skills from preintervention to approximately 10 months later were calculated. Results showed that as teacher ratings of reading improved, there were corresponding improvements in social skills. For students in peer‐mediated math interventions, increases in math fluency were correlated with improvements in self‐control. Results are discussed in the context of possible reasons for these findings and implications for practice and future research. © 2008 Wiley Periodicals, Inc.
Clinical application of proteomics approaches in vascular diseases
Authors: Wang Xiao‐Li , Fu Alex , Spiro Craig , Lee Hon‐Chi 
Published: January 2008

AbstractCardiovascular diseases are leading causes of morbidity and mortality in developed countries. Despite modern advances in science and medicine, our understanding of the factors that contribute to the development of vascular dysfunction is incomplete. Progress in treatment will depend on elucidating the complex interactions of hormones, growth factors, inflammatory agents, and oxidative stress on cell proliferation, migration, and death both in the vasculature and in circulation. Recent developments in proteomic techniques provide a promising approach for determining protein changes associated with complex diseases and permitting thorough evaluation of molecular changes associated with vascular dysfunction. Proteomic studies have revealed novel, dynamic, complex, and subtle changes of intracellular processes that are associated with the abnormal regulation of vascular function. This review provides an overview on the progress of applying proteomics to vascular diseases. We will describe the application of proteomics to clinically important vascular conditions and highlight the potential of using proteomics to advance our understanding on the mechanisms that underlie vascular diseases.
Observation of peptide differences between cancer and control in gastric juice
Authors: Chang Wei‐Chao , Hsu Ping‐I , Chen Yuan‐Yan , Hsiao Michael , Lu Pei‐Jung , Chen Chung‐Hsuan 
Published: January 2008

AbstractBiomarkers for various diseases have been extensively searched for the past 5 years. Nevertheless, most efforts were focused on the search for protein biomarkers from serum samples. In this work, we tried to look for peptide biomarkers from gastric juice samples with MALDI‐TOF‐MS. More than 200 gastric juice samples from healthy people, gastric ulcer patients, duodenal ulcer patients, and cancer patients were examined. There were clear pattern differences of mass spectra among samples from healthy people and patients with different gastric diseases. We found five peptides for gastric cancer diagnosis with high sensitivity and specificity. Sequences of these five peptides, including two pepsinogen fragments, leucine zipper protein fragment, albumin fragment, and α‐1‐antitrypsin fragment, have been identified by mass spectrometric analysis and immuno‐deplete assay with antibodies.
Proteome alterations induced in human white blood cells by consumption of Brussels sprouts: Results of a pilot intervention study
Authors: Hoelzl Christine , Lorenz Olga , Haudek Verena , Gundacker Nina , Knasmüller Siegfried , Gerner Christopher 
Published: January 2008

AbstractEpidemiological studies indicate a correlation of cruciferous vegetables consumption with reduced incidence of cancer. This study was designed to investigate molecular mechanisms, which may help to understand the beneficial effects of Brussels sprout consumption. In order to avoid the limitations of in vitro model systems, we performed a dietary intervention study with five participants. We investigated, whether sprout consumption affects the proteome profile of primary white blood cells. In order to achieve maximal sensitivity in detecting specific adaptive proteome alterations, we metabolically labelled freshly isolated cells in the presence of 35S‐methionine/cysteine and performed autoradiographic quantification of protein synthesis. Proteins were separated by 2‐DE and spots of interest were cut out, digested and identified by MS. After the intervention, we found a significant up‐regulation of the synthesis of manganese superoxide dismutase (1.56‐fold) and significant down‐regulation of the synthesis of heat shock 70 kDa protein (hsp70; 2.27‐fold). Both proteins play a role in malignant transformation of cells. Hsp‐70 is involved in the regulation of apoptosis, which leads to elimination of cancer cells, while SOD plays a key role in protection against reactive oxygen species mediated effects. Our findings indicate that the alteration of the synthesis of these proteins may be involved in the anticarcinogenic effects of cruciferous vegetables, which was observed in earlier laboratory studies with animals.