bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipido‐sterolic extract of Serenoa repens (LSESr, Permixon®) treatment affects human prostate cancer cell membrane organization
Authors: Petrangeli E. , Lenti L. , Buchetti B. , Chinzari P. , Sale P. , Salvatori L. , Ravenna L. , Lococo E. , Morgante E. , Russo A. , Frati L. , Di Silverio F. , Russo M.A. 
Published: December 2008

AbstractThe molecular mechanism by which the lipido‐sterolic extract of Serenoa repens (LSESr, Permixon®) affects prostate cells remains to be fully elucidated. In androgen‐independent PC3 prostate cancer cells, the LSESr‐induced effects on proliferation and apoptosis were evaluated by counting cells and using a FACScan cytofluorimeter. PC3 cells were stained with JC‐1 dye to detect mitochondrial membrane potential. Cell membrane lipid composition was evaluated by thin layer chromatography and gas chromatographic analysis. Akt phosphorylation was analyzed by Western blotting and cellular ultrastructure through electron microscopy. LSESr (12.5 and 25 µg/ml) administration exerted a biphasic action by both inhibiting proliferation and stimulating apoptosis. After 1 h, it caused a marked reduction in the mitochondrial potential, decreased cholesterol content and modified phospholipid composition. A decrease in phosphatidylinositol‐4,5‐bisphosphate (PIP2) level was coupled with reduced Akt phosphorylation. After 24 h, all of these effects were restored to pre‐treatment conditions; however, the saturated (SFA)/unsaturated fatty acid (UFA) ratio increased, mainly due to a significant decrease in ω6 content. The reduction in cholesterol content could be responsible for both membrane raft disruption and redistribution of signaling complexes, allowing for a decrease of PIP2 levels, reduction of Akt phosphorylation and apoptosis induction. The decrease in ω6 content appears to be responsible for the prolonged and more consistent increase in the apoptosis rate and inhibition of proliferation observed after 2–3 days of LSESr treatment. In conclusion, LSESr administration results in complex changes in cell membrane organization and fluidity of prostate cancer cells that have progressed to hormone‐independent status. J. Cell. Physiol. 219: 69–76, 2009. © 2008 Wiley‐Liss, Inc.
Oncogenic H‐Ras and PI3K signaling can inhibit E‐cadherin‐dependent apoptosis and promote cell survival after photodynamic therapy in mouse keratinocytes
Authors: Espada Jesús , Galaz Sergio , Sanz‐Rodríguez Francisco , Blázquez‐Castro Alfonso , Stockert Juan Carlos , Bagazgoitia Lorea , Jaén Pedro , González Salvador , Cano Amparo , Juarranz Ángeles 
Published: December 2008

AbstractMaintenance of E‐cadherin mediated cell–cell contacts is often required for the survival of epithelial cells and tissues. Here we report that oncogenic activation of H‐Ras in murine keratinocytes can prevent cell death induced by immunological disruption of E‐cadherin adhesion. A similar situation was observed in cells showing constitutive activation of the p110α catalytic subunit of class IA PI3K. This protective effect is associated with β‐catenin‐dependent transcription and with activation of survival factor Akt/PKB. In addition, we induced cell death by employing photodynamic therapy, using Zn‐phthalocyanine as a photosensitizer that targets E‐cadherin adhesion complexes. We have found that cell death based on this photodynamic action is also bypassed in cells showing constitutive activation of H‐Ras and p110α. Taken together, these results indicate that H‐Ras/PI3K/Akt signaling plays a key role in cell survival mediated by E‐cadherin cell–cell contacts. J. Cell. Physiol. 219: 84–93, 2009. © 2008 Wiley‐Liss, Inc.
Purification and characterization of recombinant human erythropoietin from milk of transgenic pigs
Authors: Lee Eun Gyo , Lee Seung Hui , Park Kyung Mi , Baek Jung Eun , Yeon Sun Hee , Park Jin‐Ki , Chang Won‐Kyong , Jung Joon‐Ki , Chung Bong Hyun 
Published: December 2008

AbstractBACKGROUND: Human erythropoietin (hEPO), a hydrophobic acidic glycoprotein responsible for the regulation of red blood cell production in mammals, is used for the treatment of anemia. In general, the purification of transgenic animal‐derived therapeutic proteins is not easy due to their low titer concentrations and abundant contaminant proteins. For the first time, here the purification and characterization of rhEPO from the milk of transgenic pigs are described.RESULTS: The rhEPO was purified by heparin chromatography, reverse‐phase chromatography, and gel filtration chromatography, resulting in a 16.5% yield and > 98% purity. The rhEPO purified from the milk of transgenic pigs contained less acidic isoforms and was underglycosylated in contrast to CHO‐derived rhEPO. Cell proliferation of the F‐36/EPO‐dependent cell line was proportional to the dose of transgenic pig‐derived rhEPO.CONCLUSION: Transgenic pig‐derived rhEPO with high purity was achieved after three‐step chromatography following two‐step precipitation. The transgenic pig‐derived rhEPO was demonstrated to have comparable potency with CHO‐derived rhEPO. Transgenic pig‐derived rhEPO may not be therapeutically feasible because of different glycosylation, and thus further studies are required to elucidate the effect of this aberrant glycosylation on the biological activity and stability in vivo. Copyright © 2008 Society of Chemical Industry
Bilateral femoral artery aneurysms and bilateral common femoral vein thromboses
Authors: Secko Michael A. , Stone, Michael B. 
Published: December 2008

AbstractWe report the sonographic findings of a patient with bilateral femoral artery aneurysms with concomitant bilateral deep venous thrombosis of the common femoral veins. Femoral arterial aneurysms may be associated with additional arterial aneurysms, and rarely with associated venous thrombosis. Patients presenting with femoral artery aneurysms should undergo a thorough vascular sonographic examination that includes the abdominal aorta, the iliac vessels and the deep venous system of the lower extremity. © 2008 Wiley Periodicals, Inc. J Clin Ultrasound, 2009
Geographical profiling obscene phone calls—a case study
Authors: Ebberline Jessica 
Published: December 2008

AbstractStudies on geographical profiling have typically focused on crimes with physical crime sites. This study focused on crimes with no physical crimes sites (i.e. no physical contact occurred between the victim and the offender). A single offender made obscene phone calls to 86 children during the years 1999–2000 in the south of Sweden. The locations in which obscene phone calls were received were analysed in a geographical profiling system (Dragnet) to see whether it could provide a map that showed the most likely area the offender would reside in. The purpose of this study was to explore whether geographical software such as Dragnet can be used by investigators of crimes with no physical crime locations. The result showed that the offender in this case had his home close to the region assigned the highest probability of containing home or base, which indicates that Dragnet can provide maps that can narrow down the search areas for the police in cases without physical crime locations. Copyright © 2008 John Wiley & Sons, Ltd.
Effects of two contrasting agricultural land‐use practices on nitrogen leaching in a sandy soil of Middle Germany
Authors: Böhm Christian , Landgraf Dirk , Makeschin Franz 
Published: December 2008

AbstractThe objective of this study is to evaluate different agricultural land‐use practices in terms of N leaching and to give recommendations for a sustainable agriculture on sandy soils in Middle Germany. Soil mineral N (Nmin) and leachate N were quantified at a sandy soil in N Saxony during 3 years. Two treatments were applied: intensive (I)—using inorganic and organic fertilizer and pesticides, and organic (O)—exclusively using organic fertilizer, legume‐based crop rotation, and no pesticides. Split application of mineral fertilizers did not result in substantial N losses at treatment I. Legumes induced a considerable increase of soil mineral N and particularly of leachate mineral N (Nmin_perc) at treatment O. High Nmin_perc concentrations (up to 78 mg N L–1) were observed during as well as after the cultivation of legumes. These high Nmin_perc concentrations are the reason why clearly higher Nmin_perc losses were determined at treatment O (62 kg N ha–1 y–1) compared to treatment I (23 kg N ha–1 y–1). At both treatments, the quantity of N losses was strongly affected by the precipitation rates. Concentrations and losses of dissolved organic N (DONperc) were assessed as above average at both treatments. The results suggest that the DONperc concentration is influenced by precipitation, soil coverage, and organic fertilizers. Higher values were determined in the percolation water of treatment O. The average annual DONperc losses amounted to 15 kg N ha–1 at I and to 32 kg N ha–1 at O. The average monthly percentage of DONperc losses on the loss of the dissolved total N of percolation water (DTNperc) ranged between <1% and 55% at O and between 2% and 56% at I. For the whole measuring period of 29 months, the relative amounts of DONperc of DTNperc (21% at O and 25% at I) were more or less the same for both treatments. The results show that DONperc can contribute significantly to the total N loss, confirming the importance to consider this N fraction in N‐leaching studies. It was concluded that at sandy sites, a split application of mineral fertilizers, as applied at treatment I, seems to be more expedient for limiting the N leaching losses than legume‐based crop rotations.
Methylation profile of circulating plasma DNA in patients with pancreatic cancer
Authors: Melnikov Anatoliy A. , Scholtens Denise , Talamonti Mark S. , Bentrem David J. , Levenson Victor V. 
Published: December 2008

AbstractBackground and ObjectivesDetection of pancreatic cancer by blood‐based test may improve outcomes. We sought to establish the feasibility of a blood‐based detection of pancreatic cancer through multiplexed array‐mediated analysis of DNA methylation.MethodsMethylation was assessed in each plasma sample using a panel of 56 frequently methylated genes. Methylation profiles in patients with ductal cell adenocarcinoma of the pancreas (n = 30) and healthy gender and age‐matched controls (n = 30) were compared. Methylation was determined as described previously; a composite biomarker was developed for classification of cancer and normal samples. Sensitivity and specificity of the biomarker were estimated using 25 rounds of fivefold cross‐validation.ResultsFive promoters were consistently selected for the classifier during cross‐validation and comprised the final composite biomarker Five‐fold cross‐validation results indicate 76% sensitivity and 59% specificity of the biomarker, which included promoters of CCND2, SOCS1, THBS1, PLAU, and VHL.ConclusionDifferential methylation profiling of plasma DNA can detect ductal adenocarcinoma of the pancreas with significant accuracy and should be explored further. While additional improvement of biomarkers is necessary, the blood‐based biomarker may be already useful as a first‐line detection tool. J. Surg. Oncol. 2009;99: 119–122. © 2008 Wiley‐Liss, Inc.
Quantum‐optical analogies using photonic structures
Authors: Longhi S. 
Published: December 2008

AbstractEngineered photonic waveguides have provided in the past decade an extremely rich laboratory tool to visualize with optical waves the classic analogues of a wide variety of coherent quantum phenomena encountered in atomic, molecular or condensed‐matter physics. As compared to quantum systems, optics offers the rather unique advantage of a direct mapping of the wave function evolution in coordinate space by simple fluorescence imaging or scanning tunneling optical microscopy techniques. In this contribution recent theoretical and experimental advances in the field of quantum‐optical analogies are reviewed. Special attention is devoted to some relevant optical analogies based on the use of curved photonic structures, including: coherent destruction of tunneling in driven bistable potentials; coherent population transfer and adiabatic passage in laser‐driven multilevel atomic systems; quantum decay control and Zeno dynamics; electronic Bloch oscillations and Zener tunneling, Anderson localization and dynamic localization in crystalline potentials.
Solder doped polycaprolactone scaffold enables reproducible laser tissue soldering
Authors: Bregy Amadé , Bogni Serge , Bernau Vianney J.P. , Vajtai Istvan , Vollbach Felix , Petri‐Fink Alke , Constantinescu Mihai , Hofmann Heinrich , Frenz Martin , Reinert Michael 
Published: December 2008

AbstractBackground and ObjectivesIn this in vitro feasibility study we analyzed tissue fusion using bovine serum albumin (BSA) and Indocyanine green (ICG) doped polycaprolactone (PCL) scaffolds in combination with a diode laser as energy source while focusing on the influence of irradiation power and albumin concentration on the resulting tensile strength and induced tissue damage.Materials and MethodsA porous PCL scaffold doped with either 25% or 40% (w/w) of BSA in combination with 0.1% (w/w) ICG was used to fuse rabbit aortas. Soldering energy was delivered through the vessel from the endoluminal side using a continuous wave diode laser at 808 nm via a 400 µm core fiber. Scaffold surface temperatures were analyzed with an infrared camera. Optimum parameters such as irradiation time, radiation power and temperature were determined in view of maximum tensile strength but simultaneously minimum thermally induced tissue damage. Differential scanning calorimetry (DSC) was performed to measure the influence of PCL on the denaturation temperature of BSA.ResultsOptimum parameter settings were found to be 60 seconds irradiation time and 1.5 W irradiation power resulting in tensile strengths of around 2,000 mN. Corresponding scaffold surface temperature was 117.4± 12°C. Comparison of the two BSA concentration revealed that 40% BSA scaffold resulted in significant higher tensile strength compared to the 25%. At optimum parameter settings, thermal damage was restricted to the adventitia and its interface with the outermost layer of the tunica media. The DSC showed two endothermic peaks in BSA containing samples, both strongly depending on the water content and the presence of PCL and/or ICG.ConclusionsDiode laser soldering of vascular tissue using BSA‐ICG‐PCL‐scaffolds leads to strong and reproducible tissue bonds, with vessel damage limited to the adventitia. Higher BSA content results in higher tensile strengths. The DSC‐measurements showed that BSA denaturation temperature is lowered by addition of water and/or ICG‐PCL. Lasers Surg. Med. 40:716–725, 2008. © 2008 Wiley‐Liss, Inc.
Role of HSP70 in cellular thermotolerance
Authors: Beckham Josh T. , Wilmink Gerald J. , Mackanos Mark A. , Takahashi Keiko , Contag Chris H. , Takahashi Takamune , Jansen E. Duco 
Published: December 2008

AbstractBackground and ObjectiveThermal pretreatment has been shown to condition tissue to a more severe secondary heat stress. In this research we examined the particular contribution of heat shock protein 70 (HSP70) in thermal preconditioning.Study Design/Materials and MethodsFor optimization of preshock exposures, a bioluminescent Hsp70‐luciferase reporter system in NIH3T3 cells tracked the activation of the Hsp70 gene. Cells in 96‐well plates were pretreated in a 43°C water bath for 30 minutes, followed 4 hours later with a severe heat shock at 45°C for 50 minutes. Bioluminescence was measured at 2, 4, 6, 8, and 10 hours after preshock only (PS) and at 4 hours after preshock with heatshock (PS+HS). Viability was assessed 48 hours later with a fluorescent viability dye. Preshock induced thermotolerance was then evaluated in hsp70‐containing Murine Embryo Fibroblast (+/+) cells and Hsp70‐deficient MEF cells (−/−) through an Arrhenius damage model across varying temperatures (44.5–46°C).ResultsA time gap of 4 hours between preconditioning and the thermal insult was shown to be the most effective for thermotolerance with statistical confidence of P<0.05. The benefit of preshocking was largely abrogated in Hsp70‐deficient cells. The Arrhenius data showed that preshocking leads to increases in the activation energies, Ea, and increases in frequency factors, A. The frequency factor increase was significantly greater in Hsp70‐deficient cells.ConclusionThe data shows that HSP70 contributes significantly to cellular thermotolerance but there are other pathways that provide residual thermotolerance in cells deficient in Hsp70. Lasers Surg. Med. 40:704–715, 2008. © 2008 Wiley‐Liss, Inc.
Photodynamic therapy as treatment of chronic idiopathic central serous chorioretinopathy
Authors: Tarantola Ryan M. , Law Janice C. , Recchia Franco M. , Sternberg Paul , Agarwal Anita 
Published: December 2008

AbstractBackground and ObjectiveChronic idiopathic central serous chorioretinopathy (ICSC) is characterized by persistent, recurrent, and multifocal serous detachments of the neurosensory retina. Our objective is to evaluate the visual and anatomical outcomes of patients who underwent photodynamic therapy (PDT) with Verteporfin for chronic ICSC.Study Design/Materials and MethodsA retrospective review of 13 eyes of 12 patients (mean age 55, range 45–66 years) treated with PDT for chronic ICSC between June 2004 and January 2008 was conducted. Two eyes that subsequently developed choroidal neovascularization were excluded. Best corrected visual acuity (BCVA) and clinical examination prior to PDT and at most recent follow‐up were recorded. Fluorescein angiography (FA) and optical coherence tomography (OCT) findings prior to and following treatment were reviewed.ResultsMean duration of symptoms prior to PDT was 26.7 months (range 3–108 months). Mean follow‐up duration was 21.9 months (range 2–42 months). BCVA improved in seven of eleven eyes (mean 1.7 lines, range 1–3 lines), two eyes remained unchanged, and two eyes worsened. Complete resolution of serous detachment was demonstrated in 9 of 11 eyes by FA or OCT and 10 of 11 eyes by clinical examination. One eye showed a reduction in serous detachment by OCT and clinical exam. Three eyes required two treatments for recurrent serous detachment following initial treatment.ConclusionOur data suggest that PDT leads to resolution of serous detachments and visual improvement in patients with chronic ICSC. Further follow‐up would be prudent to assess the long‐term outcomes and safety in this patient population. Lasers Surg. Med. 40:671–675, 2008. © 2008 Wiley‐Liss, Inc.
Visible 405 nm SLD light photo‐destroys methicillin‐resistant Staphylococcus aureus (MRSA) in vitro
Authors: Enwemeka Chukuka S. , Williams Deborah , Hollosi Steve , Yens David , Enwemeka Sombiri K. 
Published: December 2008

AbstractBackgroundInfections with MRSA remain a growing public health concern, prompting the need to explore alternative treatments instead of the on‐going effort to develop stronger drug‐based therapies. We studied the effect of 405 nm blue light on two strains of MRSA—US‐300 strain of CA‐MRSA and the IS853 strain of HA‐MRSA—in vitro.MethodsWe cultured and plated each strain, following which bacteria colonies were irradiated with 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 25, 30, 35, 40, 45, 50, 55, or 60 J cm−2 energy densities—just once—using a Solaris® superluminous diode (SLD) device. Specimens were incubated at 35°C for 24 hours. Then, digital images obtained were quantified to obtain colony counts and the aggregate area occupied by bacteria colonies.ResultsBlue light irradiation produced a statistically significant dose‐dependent reduction in both the number and the aggregate area of colonies formed by each bacteria strain (P<0.001). Maximum eradication of the US‐300 (92.1%) and the IS‐853 colonies (93.5%) was achieved within 9.2 and 8.4 minutes of exposure, respectively. The longer the irradiation the more bacteria were eradicated. However, the effect was non‐linear as increases of energy densities between 1.0 and 15 J cm−2 resulted in more bacteria death than similar increases between 15 and 60 J cm−2.ConclusionAt low doses, blue light photo‐destroys HA‐MRSA and CA‐MRSA in vitro; raising the prospect that phototherapy may be an effective clinical tool in the on‐going effort to stem MRSA infections. Lasers Surg. Med. 40:734–737, 2008. © 2008 Wiley‐Liss, Inc.
Correlation between antiangiogenic activity and antioxidant activity of various components from propolis
Authors: Ahn Mok‐Ryeon , Kunimasa Kazuhiro , Kumazawa Shigenori , Nakayama Tsutomu , Kaji Kazuhiko , Uto Yoshihiro , Hori Hitoshi , Nagasawa Hideko , Ohta Toshiro 
Published: December 2008

AbstractPropolis possesses various physiological activities. In this study, we examined the antiangiogenic and antioxidant activities of various components from propolis: acacetin, apigenin, artepillin C, caffeic acid phenethyl ester, chrysin, p‐coumaric acid, galangin, kaempferol, pinocembrin, and quercetin. The effects of these components were tested on in vitro models of angiogenesis, tube formation and growth of human umbilical vein endothelial cells (HUVECs). Furthermore, these components were evaluated for their antioxidant activities by 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radical‐scavenging and ferric reducing/antioxidant power (FRAP) assays. Two propolis components, caffeic acid phenethyl ester, and quercetin, possessed strong inhibitory effects on tube formation and on endothelial cell proliferation and, coincidentally, showed strong antioxidant activity. Artepillin C, galangin, and kaempferol also possessed strong antiangiogenic and antioxidant activities to a slightly less degree. In contrast, acacetin, apigenin, and pinocembrin possessed a considerable degree of antiangiogenic activities, although they showed very low antioxidant activities. From these results, we propose that components from propolis such as artepillin C, caffeic acid phenethyl ester, galangin, kaempferol, and quercetin might represent a new class of dietary‐derived antioxidative compounds with antiangiogenic activities. These propolis components may have the potential to be developed into pharmaceutical drugs for the treatment of angiogenesis‐dependent human diseases such as tumors.
Respective contribution exerted by AF‐1 and AF‐2 transactivation functions in estrogen receptor α induced transcriptional activity by isoflavones and equol: Consequence on breast cancer cell proliferation
Authors: Carreau Charlotte , Flouriot Gilles , Bennetau‐Pelissero Catherine , Potier Mylène 
Published: December 2008

AbstractEstrogens used in hormone replacement therapy regimens may increase the risk of developing breast cancer. Paradoxically, high consumption of plant‐derived phytoestrogens, particularly soybean isoflavones, is associated with a low incidence of breast cancer. To explore the molecular basis for these potentially different experimental/clinical outcomes, we investigated whether soybean isoflavones elicit distinct transcriptional actions from estrogens by performing transient transfections in different cell lines. Our results demonstrate that 17β estradiol (E2), isoflavones, and equol (EQ) effectively trigger the transcriptional activation with both estrogen receptors (ER), ERα and ERβ. ERα transcriptional activity is mediated through two transactivation domains AF‐1 and AF‐2, whose activity is tightly regulated in a cell‐type and promoter‐specific manner. Isoflavones, genistein, and daidzein (DAI), and EQ, the main estrogenic metabolite of DAI, are ERα agonists for transcriptional activation. The molecular mechanisms for ERα‐induced transcriptional activity by isoflavones and EQ involve their capacity to act mainly through AF‐1 regardless of the cell type. Therefore, our data indicate that estrogenic ligands, such as isoflavones and EQ, exert their effects on ERα transactivation similarly to the endogenous ligand E2, and suggest that the risk of estrogen‐related diseases might not be reduced by soy‐rich food and/or isoflavone‐ or EQ‐based supplements.