bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subgingival biofilm microbiome in individuals with asthma and periodontitis: Metagenomic analysis
Authors: Pinto Giselle R. , Carvalho Filho Paulo C. , Carvalho Rodrigo D. O. , Conceição Rogério R. , Fortuna Vitor , Gomes‐Filho Isaac S. , Trindade Soraya C. , Sarmento Viviane A. 
Published: March 2024

AbstractObjectiveThis observational study aimed to explore the metagenomics of subgingival biofilms in individuals with varying degrees of asthma, from severe to none, to elucidate the association between the subgingival microbiome and asthma.Materials and MethodsSubgingival biofilm samples were collected from thirty participants at the Asthma Control Program Outpatient Clinic in Bahia (ProAR). These samples were categorized into six groups based on the severity of asthma and the presence or absence of periodontitis. We employed next‐generation sequencing (Illumina MiSeq), targeting the 16S rRNA gene, to characterize the microbial communities present. Our analysis included descriptive statistics and sequencing data, evaluated using multivariate statistical methods such as the Shannon index, principal coordinate analysis, and the Bray–Curtis dissimilarity.ResultsOur findings indicate a higher prevalence of periodontally detrimental bacterial genera in individuals with severe asthma and periodontitis. Additionally, individuals with asthma, but without periodontitis, exhibited a tendency toward dysbiosis, particularly in cases of severe asthma.ConclusionThis research provides new insights into the composition of the subgingival microbiome in individuals with varying severities of asthma and periodontitis. The genera identified in this study underscore the need for further investigations to build upon these findings.
IRE1α inhibits osteogenic differentiation of mouse embryonic fibroblasts by limiting Shh signaling
Authors: Zhang Zhixiang , Zhang Xuan , Wei Xiangzhen , Yu Chengbo , Xiao Li , Liu Jianmiao , Liu Yong , Cao Yingguang , Song Ke 
Published: March 2024

AbstractObjectivesThis study aimed to investigate the effect of endoplasmic reticulum (ER) stress sensor inositol‐requiring enzyme 1α (IRE1α) on the sonic hedgehog N‐terminus (N‐Shh)‐enhanced‐osteogenic differentiation process in mouse embryonic fibroblasts (MEFs).Materials and MethodsOsteogenesis of MEFs was observed by alkaline phosphatase (ALP) staining, alizarin red staining, and Von Kossa staining assays. Activation of unfolded protein response and Shh signaling were examined using real‐time quantitative PCR and western blot assays. IRE1α‐deficient MEFs were used to explore the effect of IRE1α on N‐Shh‐driven osteogenesis.ResultsN‐Shh increased ALP activity, matrix mineralization, and the expression of Alp and Col‐I in MEFs under osteogenic conditions; notably, this was reversed when combined with the ER stress activator Tm treatment. Interestingly, the administration of N‐Shh decreased the expression of IRE1α. Abrogation of IRE1α increased the expression of Shh pathway factors in osteogenesis‐induced MEFs, contributing to the osteogenic effect of N‐Shh. Moreover, IRE1α‐deficient MEFs exhibited elevated levels of osteogenic markers.ConclusionsOur findings suggest that the IRE1α‐mediated unfolded protein response may alleviate the ossification of MEFs by attenuating Shh signaling. Our research has identified a strategy to inhibit excessive ossification, which may have clinical significance in preventing temporomandibular joint bony ankylosis.
Topical antibiotics limit depigmentation in a mouse model of vitiligo
Authors: Touni Ahmed Ahmed , Sohn Rachel , Cosgrove Cormac , Shivde Rohan S. , Dellacecca Emilia R. , Abdel‐Aziz Rasha T. A. , Cedercreutz Kettil , Green Stefan J. , Abdel‐Wahab Hossam , Le Poole I. Caroline 
Published: March 2024

AbstractOral neomycin administration impacts the gut microbiome and delays vitiligo development in mice, and topical antibiotics may likewise allow the microbiome to preserve skin health and delay depigmentation. Here, we examined the effects of 6‐week topical antibiotic treatment on vitiligo‐prone pmel‐1 mice. Bacitracin, Neosporin, or Vaseline were applied to one denuded flank, while the contralateral flank was treated with Vaseline in all mice. Ventral depigmentation was quantified weekly. We found that topical Neosporin treatment significantly reduced depigmentation and exhibited effects beyond the treated area, while Bacitracin ointment had no effect. Stool samples collected from four representative mice/group during treatment revealed that Neosporin treatment aligned with reduced abundance of the Alistipes genus in the gut, while relevant changes to the skin microbiome at end point were less apparent. Either antibiotic treatment led to reduced expression of MR1, potentially limiting mucosal‐associated invariant T‐cell activation, while Neosporin‐treated skin selectively revealed significantly reduced CD8+ T‐cell abundance. The latter finding aligned with reduced expression of multiple inflammatory markers and markedly increased regulatory T‐cell density. Our studies on favorable skin and oral antibiotic treatment share the neomycin compound, and in either case, microbial changes were most apparent in stool samples. Taken together, neomycin‐containing antibiotic applications can mediate skin Treg infiltration to limit vitiligo development. Our study highlights the therapeutic potential of short‐term antibiotic applications to limit depigmentation vitiligo.
Forb stability, dwarf shrub stability and species asynchrony regulate ecosystem stability along an experimental precipitation gradient in a semi‐arid desert grassland
Authors: Zhang L. , Gao J. , Zhao R. , Wang J. , Hao L. , Wang M. 
Published: March 2024

Abstract
Precipitation pattern changes may affect plant biodiversity, which could impact ecosystem stability. However, the effects of changes in precipitation regime on ecosystem stability and their potential mechanisms are still unclear.
We conducted a 3‐year field manipulation experiment with five precipitation treatments (−40%, −20%, 0% (CK), +20% and +40% of ambient growing season precipitation) in a semi‐arid desert grassland to examine the effects of precipitation alterations on functional group stability, species asynchrony, and diversity, and the underlying mchanisms of ecosystem stability using structural equation modelling.
Alterations in precipitation had different effects on community biomass and functional group biomass. Moreover, ecosystem stability was mainly driven by forb stability (path coefficient = 0.79). Changes in precipitation had significant effects on soil dissolved inorganic N (P < 0.01) further affecting ecosystem stability through species asynchrony (path coefficient = 0.25). Dwarf shrubs had a stabilizing effect on ecosystem stability (path coefficient = 0.32), mainly via deep roots. Ecosystem stability tended to be lower in the −40% (4.72) and +40% (2.74) precipitation treatments. The common reduction in species asynchrony and stability of forb and dwarf shrub functional groups resulted in lower ecosystem stability under the −40% treatment. The lower stability under the +40% treatment might be ascribed to unimproved dwarf shrub stability. Higher dwarf shrub and forb stability contributed to higher ecosystem stability under normal precipitation changes (±20% treatments) and CK. Species diversity was not a crucial driver of ecosystem stability.
Our results indicate that precipitation alteration can regulate ecosystem stability via functional group stability (e.g. forb stability, dwarf shrub stability) and species asynchrony in a semiarid desert grassland.
A comprehensive approach for evaluating salinity stress tolerance in brinjal (Solanum melongena L.) germplasm using membership function value
Authors: Gyanagoudar Harsha Shekarappa , Hatiya Singh Tejavathu , Guhey Arti , Dharmappa Prathibha Machagondanahalli , Seetharamaiah Shivashankara Kodthalu 
Published: March 2024

AbstractSalinity is a major stress factor affecting plant growth and development, which limits the productivity of vegetable crops. Brinjal (Solanum melongena L.), an important vegetable cultivated across the globe is susceptible to salinity stress. In the present study, the salinity tolerance response of 110 brinjal germplasm lines was evaluated at the germination stage using the membership function value (MFV). The MFV is a comprehensive index that integrates salt‐tolerance indices of germination parameters. The brinjal germplasms were classified into highly salt‐tolerant (>0.79), salt‐tolerant (0.79–0.65), moderately salt‐tolerant (0.64–0.21), salt‐sensitive (0.20–0.07), and highly salt‐sensitive (<0.07) based on their mean MFV. Among all the traits examined, germination percentage (0.874) and vigour index‐I (0.808) were the most reliable traits for assessing salinity tolerance, showing a higher correlation with mean MFV. Furthermore, a comprehensive mathematical model was developed for evaluating the salt‐tolerance of the brinjal germplasm. We validated our model by evaluating the brinjal germplasm at the seedling stage through a hydroponic experiment, and a strong positive correlation was observed between growth parameters at the germination and seedling stages. Salt‐tolerant genotypes showed higher chlorophyll content, photosynthetic performance and biomass accumulation with lower canopy temperature (1.57°C) under salinity compared to susceptible genotypes (2.62°C). These findings provide valuable insights into the salinity tolerance of the brinjal germplasm, and identified potential candidates to elucidate the molecular mechanisms and develop salinity‐tolerant cultivars. To our knowledge, this is the first report using a mathematical model based on MFV to evaluate the salt‐tolerance of any vegetable crop.
MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis
Authors: Liu Yuan , Luo Cong , Lan Moying , Guo Yihang , Li Ruoyan , Liang Rongzhen , Chen Shuquan , Zhong Junjie , Li Baijun , Xie Fangfang , Chen Canbin , He Xinhua 
Published: March 2024

AbstractThe CONSTANS/CONSTANS‐Like (CO/COL) family has been shown to play important roles in flowering, stress tolerance, fruit development and ripening in higher plants. In this study, three COL genes, MiCOL6, MiCOL7A and MiCOL7B, which each contain only one CCT domain, were isolated from mango (Mangifera indica), and their functions were investigated. MiCOL7A and MiCOL7B were expressed mainly at 20 days after flowering (DAF), and all three genes were highly expressed during the flowering induction period. The expression levels of the three genes were affected by light conditions, but only MiCOL6 exhibited a clear circadian rhythm. Overexpression of MiCOL6 promoted earlier flowering, while overexpression of MiCOL7A or MiCOL7B delayed flowering compared to that in the control lines of Arabidopsis thaliana under long‐day (LD) and short‐day (SD) conditions. Overexpressing MiCOL6, MiCOL7A or MiCOL7B in transgenic plants increased superoxide dismutase (SOD) and proline levels, decreased malondialdehyde (MAD) levels, and improved survival under drought and salt stress. In addition, yeast two‐hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) analyses showed that the MiCOL6, MiCOL7A and MiCOL7B proteins interact with several stress‐ and flower‐related proteins. This work demonstrates the functions of MiCOL6, MiCOL7A and MiCOL7B and provides a foundation for further research on the role of mango COL genes in flowering regulation and the abiotic stress response.
Translation and validation of ageism scale for dental students into Malay language
Authors: Yong Tee Yee , Zhen Chen Yik , John Jacob , Danaee Mahmoud , Marchini Leonardo 
Published: March 2024

AbstractAimsIdentifying ageism among dental students is essential in gerodontology courses. This study aimed to perform a preliminary validation of a Malay version of the ageism scale for dental students (ASDS‐My).Methods and ResultsThe 27‐item ASDS were translated into Malay language and completed by 168 dental students. Exploratory factor analysis (EFA) with Promax rotation, Polychoric Correlation Matrix and Principal Axis Factoring was conducted. Internal consistency reliability and discriminative validity were analysed. Criterion validity was measured by comparing ASDS‐My with Fraboni Scale of Ageism (FSA). A Kaiser–Meyer–Olkin of 0.612 and a Bartlett's Test of Sphericity yielding p < .001 confirmed the adequate factorability. EFA revealed a 15‐items scale distributed into five‐factors explaining 54.5% of overall variance, with acceptable reliability. The two factors involved cost‐benefit of dental treatment on older patients and challenges during medical history taking. The third, fourth and fifth factors explained attitude towards older patients, negative views of older patients and exposure of dental students to Gerodontology training and experiences respectively. Most factors were significantly positively related to FSA. Discriminative validity revealed significant differences for gender, place of stay and year of study.ConclusionPreliminary validation of the ASDS‐My produced a five‐factors 15‐items scale with acceptable validity and reliability.
Mapeamento temático e tendências conceituais dos estudos sobre a participação social em saúde na América
Authors: Viana Machado Frederico , Rech Carla Michele , Silveira Pinto Rodrigo , Romão Wagner de Melo , Maria Marques Matias Manuelle , Freitas Gabriele Carvalho de , Kujawa Henrique 
Published: March 2024

RESUMO: Introdução/objetivos: Este trabalho mapeia os termos e conceitos mais utilizados em artigos sobre participação em saúde buscando identificar seu tratamento ao longo do tempo, bem como a relação, a organização e a distribuição temporal das conexões temáticas e áreas de conhecimento, considerando a diversidade de temas e conceitos presentes na literatura nas Américas. Metodologia: Foi realizada uma análise bibliométrica de artigos em inglês, espanhol e português, extraídos de buscas na BVS, Pubmed, SCOPUS, WOS e SciELO, consolidando uma base de dados com 641 referências. Com o uso do VOSViewer aplicamos a análise de coocorrência de palavras baseada em dados de texto (título e resumo) e coocorrência de palavras-chave. Resultados: Foram mapeadas duas grandes abordagens para a participação, uma mais relacionada à comunidade e outra às instituições políticas públicas. A análise temporal pode identificar a evolução e a frequência da utilização de conceitos, métodos e temas nas últimas décadas. Conclusões: O desenvolvimento histórico, o acúmulo de produções e a diversificação do campo coincidem com uma maior especificidade conceitual e a formação de consensos teórico. Os descritores metodológicos indicam a hegemonia das abordagens qualitativas, e a consolidação da participação em saúde como um objeto de investigação científica, um método de produção de conhecimento e uma ferramenta capaz de contribuir para a gestão e o aprimoramento das políticas públicas.
PALAVRAS-CHAVE: Bibliometria; Saúde Pública; Participação em Saúde; Engajamento Comunitário;
In Situ Measurement of Oil Reservoir and Oil Layers by Fluorescence Technique in a Ball-on-Disc Test Rig
Authors: Liang He , Li Chuang , Wang Wenzhong , Guo Yulong 
Published: March 2024

Abstract
The major oil supply for the ball-on-disc contact is provided by the lubricating oil reservoir and the oil ridges; however, the regularity of their changes has not been thoroughly investigated. In this study, the laser-induced fluorescence technique was adopted to determine the film distribution of the oil reservoir and the layer thickness of the oil ridges on the free surface. It clearly depicts the changes in the oil reservoir and reveals the mechanism of the oil reflow surrounding the contact region under various driving actions. The results may give a new perspective and a fuller understanding of the lubricating oil supply by revealing additional information about the oil supply surrounding the contact region.
Study on Fretting Wear Properties of GCr15 Steel Via Ultrasonic Surface Rolling Process
Authors: Sun Xinhua , Xia Dianxiu , Zhang Mingyuan , Wang Shouren , Zhang Yunhe , Cai Yuquan , Si Guyi , Li Shuqi , Zhang Xintao , Zhang Jian 
Published: March 2024

Abstract
Ultrasonic surface rolling (USR) was applied to GCr15 steel with different static loads and passes to improve the friction and wear properties, and then the fretting wear mechanism of GCr15 steel after USR treatment was systematically investigated. The results showed that the specimens treated by the USR had lower surface roughness and significantly increased compressive residual stress and microhardness. Furthermore, severe plastic deformation occurred in the surface layer of the specimen, which refined the grains and increased the density of high- and low-angle grain boundaries. Besides, the results of the fretting test showed that the USR treated specimens had lower wear volume, dissipated energy, and steady-state friction coefficient. The fretting wear resistance increased with the static load and the number of passes. The fretting wear mechanism changed from abrasive wear and severe adhesive wear to slight fatigue wear and abrasive wear owing to the use of the USR treatment. Surface smoothing and hardening are responsible for the improvement in the fretting wear properties of GCr15 steel for USR treatment.
Propagation of Uncertainty in Experimental Dynamic Coefficients of Fluid Film Journal Bearings
Authors: Herrera Pedro , Goyne Christopher , Rockwell Robert 
Published: March 2024

Abstract
The stiffness and damping coefficients of fluid film bearings play a key role in predicting levels of vibration and stability margins in high-performance industrial rotating machinery. However, variability in the coefficients calculated by numerical bearing codes creates inaccuracies in the rotodynamic predictions. Therefore, there is a strong need for accurate experimental measurement of bearing coefficients for validation purposes. This work examines new propagation uncertainty strategies in bearing coefficients estimation, and for the first time examines the effect of the nonlinearity of the dynamic coefficients on the experimental uncertainty estimated by the Taylor Series Method. The Montecarlo method is presented as a more accurate approach to estimating experimental uncertainty. The results of the analyses are compared to published values from previously reported studies. This paper also proposes a novel method to convert the random behavior in the output of a sensor in the time domain to the frequency domain. It is found this conversion is quite beneficial for the accuracy of the coefficients; in one example, uncertainty estimations of ±84% are reduced to just ±6%, when using the proposed method. This work also reveals that the uncertainty estimations from the Taylor Series Method are not entirely reliable, without additional checks of nonlinearity, and that converting data to the frequency domain, by using the novel method here, is useful for achieving smaller uncertainty estimations than with traditional methodology.
Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens
Authors: Bogaerts Bert , Van den Bossche An , Verhaegen Bavo , Delbrassinne Laurence , Mattheus Wesley , Nouws Stéphanie , Godfroid Maxime , Hoffman Stefan , Roosens Nancy H. C. , De Keersmaecker Sigrid C. J. , Vanneste Kevin 
Published: March 2024

ABSTRACT

Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, long-read Oxford Nanopore Technologies (ONT) sequencing has gained prominence and may offer advantages over short-read sequencing, particularly with the recent introduction of the R10 chemistry, which promises much lower error rates than the R9 chemistry. However, limited information is available on its performance for bacterial single-nucleotide polymorphism (SNP)-based outbreak investigation. We present an open-source workflow, Prokaryotic Awesome variant Calling Utility (PACU) (
https://github.com/BioinformaticsPlatformWIV-ISP/PACU
), for constructing SNP phylogenies using Illumina and/or ONT R9/R10 sequencing data. The workflow was evaluated using outbreak data sets of Shiga toxin-producing
Escherichia coli
and
Listeria monocytogenes
by comparing ONT R9 and R10 with Illumina data. The performance of each sequencing technology was evaluated not only separately but also by integrating samples sequenced by different technologies/chemistries into the same phylogenomic analysis. Additionally, the minimum sequencing time required to obtain accurate phylogenetic results using nanopore sequencing was evaluated. PACU allowed accurate identification of outbreak clusters for both species using all technologies/chemistries, but ONT R9 results deviated slightly more from the Illumina results. ONT R10 results showed trends very similar to Illumina, and we found that integrating data sets sequenced by either Illumina or ONT R10 for different isolates into the same analysis produced stable and highly accurate phylogenomic results. The resulting phylogenies for these two outbreaks stabilized after ~20 hours of sequencing for ONT R9 and ~8 hours for ONT R10. This study provides a proof of concept for using ONT R10, either in isolation or in combination with Illumina, for rapid and accurate bacterial SNP-based outbreak investigation.