bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prospective study of dietary flavonoid intake and incidence of epithelial ovarian cancer
Authors: Gates Margaret A. , Tworoger Shelley S. , Hecht Jonathan L. , De Vivo Immaculata , Rosner Bernard , Hankinson Susan E. 
Published: April 2007

AbstractFlavonoids are antioxidant compounds found in plants, including fruits, vegetables and tea. No prior prospective studies have examined the association between intake of flavonoids in the flavonol and flavone subclasses and ovarian cancer risk. We analyzed the association between intake of 5 common dietary flavonoids and incidence of epithelial ovarian cancer among 66,940 women in the Nurses' Health Study. We calculated each participant's intake of myricetin, kaempferol, quercetin, luteolin and apigenin from dietary data collected at multiple time points, and used Cox proportional hazards regression to model the incidence rate ratio (RR) of ovarian cancer for each quintile of intake. Our analysis included 347 cases diagnosed between 1984 and 2002, and 950,347 person‐years of follow‐up. There was no clear association between total intake of the 5 flavonoids examined and incidence of ovarian cancer (RR = 0.75 for the highestversuslowest quintile, 95% confidence interval [CI] = 0.51–1.09). However, there was a significant 40% decrease in ovarian cancer incidence for the highestversuslowest quintile of kaempferol intake (RR = 0.60, 95% CI = 0.42–0.87;p‐trend = 0.002), and a significant 34% decrease in incidence for the highestversuslowest quintile of luteolin intake (RR = 0.66, 95% CI = 0.49–0.91;p‐trend = 0.01). There was evidence of an inverse association with consumption of tea (nonherbal) and broccoli, the primary contributors to kaempferol intake in our population. These data suggest that dietary intake of certain flavonoids may reduce ovarian cancer risk, although additional prospective studies are needed to further evaluate this association. If confirmed, these results would provide an important target for ovarian cancer prevention. © 2007 Wiley‐Liss, Inc.
Effectiveness of preventive interventions in BRCA1/2 gene mutation carriers: A systematic review
Authors: Bermejo‐Pérez M.J. , Márquez‐Calderón S. , Llanos‐Méndez A. 
Published: April 2007

AbstractA systematic review of the literature was conducted to assess the outcomes of preventive interventions (prophylactic surgery, intensive cancer screening, and chemoprevention) in women who carry mutations in BRCA1/2 genes, in terms of reducing breast and gynaecological cancer incidence and/or mortality. A search for relevant articles published between 1996 and 2005 (inclusive) was run on Medline, Embase and other databases. From the 749 journal articles retrieved from this search strategy, 18 studies were eligible for this review (2 systematic reviews, 10 cohort studies and 6 case‐control studies). The critical appraisal of the studies was performed by two independent reviewers with a list of ad hoc selected criteria. The synthesis of results was qualitative. Mastectomy and prophylactic gynaecological surgery (oophorectomy or salpingo‐oophorectomy) reduced breast and gynaecological cancer incidence in carriers of BRCA mutations, by comparison to surveillance. However, all the studies presented flaws in internal and external validity, none of these preventive interventions is risk‐free, and protection against breast and gynaecological cancer, as well as other cancers linked to BRCA mutations, is incomplete. No studies comparing surveillance programmes of varying intensity were found. Exposure to drugs (tamoxifen, and oral contraceptives) in women carrying BRCA mutations was assessed through a limited number of papers. All of these were case‐control studies with prevalent cases and presented major methodological flaws. © 2007 Wiley‐Liss, Inc.
The development and evaluation of the intervention model for the Florida Infant Mental Health Pilot Program
Authors: Osofsky Joy D. , Kronenberg Mindy , Hammer Jill Hayes , Lederman Judge Cindy , Katz Lynne , Adams Sandra , Graham Mimi , Hogan Anne 
Published: April 2007

AbstractThe focus of this paper is on the development and evaluation of an intervention model for Florida's Infant and Young Child Mental Health Pilot Program, designed to identify families with children at risk for abuse and neglect, and to provide clinical evaluation and treatment services. The evaluation model, intervention strategies, and results presented in this paper are all part of the Florida pilot project developed as a response to the recommendations of the state's Strategic Plan for Infant Mental Health. Funded by the Florida legislature, the 3‐year, multisite pilot was designed to provide earlier identification, better evaluation, and more effective treatment services for high‐risk children under the age of three. The target population was children either at risk for out‐of‐home placement due to abuse and neglect, or those already in the child welfare system or adjudicated dependent by the state. The goals of the pilot project were: 1) to reduce the occurrence and re‐occurrence of abuse and neglect; 2) to enhance the child's developmental functioning; 3) to improve the parent‐child relationship; 4) to increase expeditious permanency placements; 5) to develop a model for intervention and treatment that could potentially be replicated in different sites; and 6) to document the components of a quality infant mental health intervention model and evaluate its effectiveness.
Dominance among unfamiliar peers starts in infancy
Authors: Plusquellec Pierrich , François Nathe , Boivin Michel , Perusse Daniel , Tremblay Richard E. 
Published: April 2007

AbstractDominance has been conceived for a long time in terms of asymmetry in agonistic conflicts. But this conception has shown its limits in the complexity of children's social worlds. Today, dominance is rather conceived in terms of asymmetry in resource control within children's dyads. Some observational studies have been done in this context, but only on small groups of familiar children and often up to 3 years of age. Our aim was to reveal whether asymmetry in resource control would occur between younger children who met each other for the first time in the presence of an attractive toy. We observed 201 dyads of 19‐month‐old unfamiliar peers in four repetitive competitive sessions for a toy. Resource control and asymmetry between participants were highly consistent between sessions. The level of asymmetry in resource control between participants was very high. This asymmetry was influenced neither by gender nor by age, and coercive behaviors in the situation, physical aggression rated by parents, birth order, and day‐care habits did not account for the asymmetry in our setting. However, body size at birth (although not at the time of testing) and anxiety rated by parents contributed significantly to the asymmetry in resource control.
Mothers' representations of their infants and parenting behavior: Associations with personal and social‐contextual variables in a high‐risk sample
Authors: Sokolowski Margaret Szewczyk , Hans Sydney L. , Bernstein Victor J. , Cox Suzanne M. 
Published: April 2007

AbstractAssociations between mothers' psychological distress, experiences of verbal and violent relational conflict, representations of the relationships with their infants, and parenting behavior were examined in 100 African American mothers of 17‐ to 20‐month‐old infants. Maternal representations of infants were assessed via the Working Model of the Child Interview (WMCI; Zeanah, Benoit, Hirshberg, & Barton, 1986). Results showed that mothers who experienced more conflict with their own mothers had increased odds of having disengaged representations of the relationship with their infants. Mothers who experienced more conflict with their infants' fathers had increased odds of having distorted views of their infants. Mothers who reported more hostile psychological symptomalogy had greater odds of not having a balanced representation of their infants (distorted or disengaged). Additionally, mothers with disengaged representations were less sensitive, more passive, and used less encouragement and guidance with their children. Maternal hostility and conflict were directly related to parenting and were not mediated by representations. Results show that representations and parenting behavior are linked and multiply determined.
Novel chlorhexidine releasing system developed from thermosensitive vinyl ether‐based hydrogels
Authors: Kiremitçi Arlin S. , Çiftçi Arzu , Özalp Meral , Gümüşderelioğlu Menemşe 
Published: April 2007

AbstractThe aim of this study is to determine the effective concentrations of chlorhexidine on the release for prolonged periods of time from a novel hydrogel system. A hydrogel that exhibits a volume phase transition in response to temperature was synthesized by radiation copolymerization of ethylene glycol vinyl ether and butyl vinyl ether in the presence of crosslinking agent, diethylene glycol divinyl ether. Hydrogel samples in the disc form (diameter, 10 mm and height, 1.5 mm) were utilized as a matrix for the release of an antimicrobial agent, chlorhexidine diacetate. Chlorhexidine loading into the hydrogel was performed by water sorption at 4°C, which allows high swelling and thus high loading capacity, i.e., ∼36 mg drug per gram of dry gel. Chlorhexidine release was examined as short‐term (24 h) and long‐term (27 days) by UV spectrophotometer. Microbial studies were carried out by micro‐dilution method in order to determine the effectiveness of the drug release. Minimum inhibitory concentration values for the pathogens of Streptococcus mutans and Lactobacillus casei were determined. The long‐term chlorhexidine release is initially very fast. After that, the drug release reaches a slow but a steady rate. Such a release pattern provides an effective drug release. The prolonged release of chlorhexidine is continued up to the 27th day. MIC values for the two pathogens have been shown that the release rate from disc is effective to inhibit the growth of pathogens. These in vitro drug release results suggested that the thermosensitive hydrogelic system developed in this study can be evaluated as a delivery system for the release of chlorhexidine. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007
MicroRNA‐223 is a key factor in osteoclast differentiation
Authors: Sugatani T. , Hruska K.A. 
Published: April 2007

AbstractMicroRNAs (miRNAs) are a class of noncording RNAs that control gene expression by translational inhibition and messenger RNAs (mRNAs) degradation in plants and animals. Although miRNAs have been implicated in developmental and homeostatic events of vertebrates and invertebrates, the role of miRNAs in bone metabolism has not been explored. Here, we show that microRNA‐223 (miR‐223) is expressed in RAW264.7 cells, mouse osteoclast precursor cell lines, and plays a critical role in osteoclast differentiation. We constructed miR‐223 short interfering RNA (siRNA) or precursor miR‐223 (pre‐miR‐223) overexpression retroviral vectors, and established miR‐223 knockdown by siRNA or pre‐miR‐223 overexpression in stably infected RAW264.7 cells. Tartrate‐resistant acid phosphatase (TRAP)‐positive multinucleated cells were observed in miR‐223 knockdown cells as well as control cells. In contrast, pre‐miR‐223 overexpression completely blocked TRAP‐positive multinucleated cell formation compared with control cells. Apoptotic cells were not observed in this study. Our results indicate that miR‐223 plays an essential role during osteoclast differentiation, and miR‐223 might be a viable therapeutic target for a range of bone metabolic disorders with excess osteoclast activity. J. Cell. Biochem. 101: 996–999, 2007. © 2007 Wiley‐Liss, Inc.
PKB/Akt inhibits ceramide‐induced apoptosis in neuroblastoma cells by blocking apoptosis‐inducing factor (AIF) translocation
Authors: Kim Nam Hyun , Kim Kyunghoon , Park Weon Seo , Son Hyeon S. , Bae Youngmee 
Published: April 2007

AbstractCeramide is a sphingolipid that is abundant in the plasma membrane of neuronal cells and is thought to have regulatory roles in cell differentiation and cell death. Ceramide is known to induce apoptosis in a variety of different cell types, whereas the physiological significance of gangliosides, another class of sphingolipids, in these processes is still unclear. We examined the mechanisms of ceramide‐induced cell death using a human neuroblastoma cell line. Treatment of the human neuroblastoma cell line SH‐SY5Y with ceramide induced dephosphorylation of the PKB/Akt kinase and subsequent mitochondrial dysfunction. In addition, ceramide‐induced neuronal cell death was not completely blocked by inhibition of caspase activity. This incomplete inhibition appeared to be attributable to the translocation of apoptosis‐inducing factor to the nucleus. Furthermore, overexpression of active PKB/Akt or Bcl‐2 successfully blocked ceramide‐induced neuronal cell death through inhibition of the translocation of apoptosis‐inducing factor. J. Cell. Biochem. 102: 1160–1170, 2007. © 2007 Wiley‐Liss, Inc.
The mouse C/EBPδ gene promoter is regulated by STAT3 and Sp1 transcriptional activators, chromatin remodeling and c‐Myc repression
Authors: Zhang Yingjie , Sif Said , DeWille Jim 
Published: April 2007

AbstractCCAAT/enhancer binding proteinδ (C/EBPδ) gene transcription is highly induced in G0 growth arrested mammary epithelial cells and “loss of function” alterations in C/EBPδ have been reported in human breast cancer. To gain a better understanding of the positive and negative factors that control C/EBPδ gene expression we investigated the role of transcriptional activators, coactivators, repressors, histone modifications, chromatin remodeling and basal transcriptional machinery components in growing and growth arrested HC11 mouse mammary epithelial cells. Growth arrest treatments result in increased STAT3 activation (pSTAT3) and increased C/EBPδ expression. Co‐immunoprecipitation and chromatin immunoprecipitation (ChIP) assays demonstrated that pSTAT3 and Sp1 interact and bind to the transcriptionally active C/EBPδ promoter. ChIP assays performed under exponentially growing (C/EBPδ non‐expressing) conditions demonstrated that the C/EBPδ promoter is preloaded with transcriptional activators (Sp1 and CREB) and transcriptional machinery components (TBP and RNA Pol II). In contrast, under G0 growth arrest (C/EBPδ expressing) conditions ChIP analysis detected pSTAT3, Sp1, NCoA/SRC1, CBP/p300, pCREB, TBP, and serine 2 phosphorylated Pol II (pPol II) in association with the C/EBPδ proximal promoter. C/EBPδ promoter‐associated histone post‐translational modification analysis revealed histone H3 and H4 acetylation and methylation patterns consistent with a constitutively “open” chromatin conformation. Chromatin remodeling experiments demonstrated that BRG1, the ATPase component of the SWI/SNF chromatin remodeling complex, is required for C/EBPδ transcription. Finally, C/EBPδ expression is repressed in proliferating mammary epithelial cells by c‐Myc via a mechanism that involves the binding of c‐Myc:Max dimers to C/EBPδ promoter‐bound Miz‐1. These results provide a molecular model of C/EBPδ transcriptional regulation under G0 growth arrest conditions. J. Cell. Biochem. 102: 1256–1270, 2007. © 2007 Wiley‐Liss, Inc.
Paradoxical roles for lysyl oxidases in cancer—A prospect
Authors: Payne Stacey L. , Hendrix Mary J.C. , Kirschmann Dawn A. 
Published: April 2007

AbstractLysyl oxidase (LOX) is an extracellular matrix (ECM) enzyme that catalyzes the cross‐linking of collagens or elastin in the extracellular compartment, thereby regulating the tensile strength of tissues. However, recent reports have demonstrated novel roles for LOX, including the ability to regulate gene transcription, motility/migration, and cell adhesion. These diverse functions have led researchers to hypothesize that LOX may have multiple roles affecting both extra‐ and intracellular cell function(s). Particularly noteworthy is aberrant LOX expression and activity that have been observed in various cancerous tissues and neoplastic cell lines. Both down and upregulation of LOX in tumor tissues and cancer cell lines have been described, suggesting a dual role for LOX as a tumor suppressor, as well as a metastasis promoter gene—creating a conundrum within the LOX research field. Here, we review the body of evidence on LOX gene expression, regulation, and function(s) in various cancer cell types and tissues, as well as stromal–tumor cell interactions. Lastly, we will examine putative mechanisms in which LOX facilitates breast cancer invasion and metastasis. Taken together, the literature demonstrates the increasingly important role(s) that LOX may play in regulating tumor progression and the necessity to elucidate its myriad mechanisms of action in order to identify potentially novel therapeutics. J. Cell. Biochem. 101: 1338–1354, 2007. © 2007 Wiley‐Liss, Inc.
Purification and growth of endothelial progenitor cells from murine bone marrow mononuclear cells
Authors: Wang Qi Ru , Wang Bao He , Huang Yan Hong , Dai Guo , Li Wei Ming , Yan Qi 
Published: April 2007

AbstractThis study reports the culture and purification of murine bone marrow endothelial progenitor cells (EPCs) using endothelial cell‐conditioned medium (EC‐CM). Endothelial‐like cells appeared at day 5 in culture of bone marrow mononuclear cells in the presence of EC‐CM in the culture system, and these cells incorporated acetylated low‐density lipoproteins (Ac‐LDL) and reacted with endothelial‐specific Ulex Europaeus Lectin. Continued incubation of these cells at low density with EC‐CM for longer than 10 days resulted in the formation of endothelial cell colonies which gave rise to colonies of endothelial progeny and can be passed for many generations in the EC‐CM culture system. Cells derived from these colonies expressed endothelial cell markers such as vWF and CD31, incorporated Dil‐Ac‐LDL, stained positive for Ulex Europaeus Lectin, formed capillary‐like structures on Matrigel, and demonstrated a high proliferative capacity in culture. These bone marrow‐derived adherent cells were identified as EPCs. The purification and the formation of EPC colonies by using EC‐CM were associated with the cytokines secreted in the EC‐CM. VEGF, bFGF, and GM‐CSF in the EC‐CM stimulated the proliferation and growth of EPCs, whereas AcSDKP (tetrapeptide NAc‐Ser‐Asp‐Lys‐Pro) in EC‐CM suppressed the growth of mesenchymal stem cells (MSC) and fibroblasts. This approach is efficient for isolation/purification and outgrowth of bone marrow EPCs in vitro, a very important cell source in angiogenic therapies and regenerative medicine. J. Cell. Biochem. 103: 21–29, 2008. © 2007 Wiley‐Liss, Inc.
Structure and bonding of Ag(I)–DNA base complexes and Ag(I)–adenine–cytosine mispairs: An ab Initio study
Authors: Schreiber Marko , González Leticia 
Published: April 2007

AbstractHigh levelab initiocalculations have been carried out to characterize the structure, bonding and energetics of Ag(I)–DNA base complexes, including adenine or cytosine, as well as Ag(I)–adenine–cytosine mispairs. The interactions of the Ag cation in all binding sites of all adenine and cytosine tautomers have been considered. The calculations show that in gas phase the canonical form of cytosine is stabilized upon metalation, whereas the lowest energy structure of Ag–adenine correspond to a rare tautomer. Interestingly, the theoretical inspection of metalated adenine–cytosine mispair reveals that the most stable structures are formed from the canonical cytosine and adenine tautomers. The lowest energy structure is planar with adenine and cytosine hydrogen‐bonded. Within few kcal/mol nonplanar, conformationally very flexible structures are found, in which the Ag(I) crosslinks an endocyclic nitrogen of adenine and the oxygen of cytosine. Metalated reverse‐Wobble type of structures, on the contrary, are predicted much higher in energy.
WATGEN: An algorithm for modeling water networks at protein–protein interfaces
Authors: Bui Huynh‐Hoa , Schiewe Alexandra J. , Haworth Ian S. 
Published: April 2007

AbstractWater molecules at protein–protein interfaces contribute to the close packing of atoms and ensure complementarity between the protein surfaces, as well as mediating polar interactions. Therefore, modeling of interface water is of importance in understanding the structural basis of biomolecular association. We present an algorithm, WATGEN, which predicts locations for water molecules at a protein–protein or protein–peptide interface, given the atomic coordinates of the protein and peptide. A key element of the WATGEN algorithm is the prediction of water sites that can form multiple hydrogen bonds that bridge the binding interface. Trial calculations were performed on water networks predicted by WATGEN at 126 protein–peptide interfaces (X‐ray resolutions ≤ 2.0 Å), using different criteria for water placement. The energies of the predicted water networks were evaluated in AMBER8 and used in the choice of parameters for WATGEN. The 126 interfaces include 1264 experimentally determined bridging water sites, and the WATGEN algorithm predicts 72 and 88% of these sites within 1.5 and 2.0 Å, respectively. The predicted number of water molecules at each interface was much higher than the number of water molecules identified experimentally. Therefore, random placement of the same number of water molecules as that predicted at each interface was performed as a control, and resulted in only 22 and 40% of water sites placed within 1.5 and 2.0 Å of experimental sites, respectively. Based on these data, we conclude that WATGEN can accurately predict the location of water molecules at a protein–peptide interface, and this may be of value for understanding the energetics and specificity of biomolecular association. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Co‐localization of the channel activating protease prostasin/(CAP1/PRSS8) with its candidate activator, matriptase
Authors: List Karin , Hobson John P. , Molinolo Alfredo , Bugge Thomas H. 
Published: April 2007

AbstractProstasin (CAP1/PRSS8) is a glycosylphosphatidylinositol‐anchored membrane serine protease believed to be critical for the regulation of epithelial sodium channel (ENaC) activity. Prostasin is synthesized as an inactive zymogen that requires a site‐specific endoproteolytic cleavage to be converted to an active protease. We have recently reported that the tumor‐associated type II transmembrane serine protease, matriptase is necessary and sufficient for prostasin activation in the epidermis. In this study, the interrelationship between the two membrane serine proteases was investigated further by using enzymatic gene trapping combined with immunohistochemistry to delineate the spatial expression of matriptase and prostasin in mouse tissues. We utilized a knock‐in mouse with a promoterless β‐galactosidase marker gene inserted into the matriptase locus, as a unique tool for precise assessment of endogenous matriptase expression. The spatial expression of matriptase and prostasin in mouse tissues was delineated by combining in situ β‐galactosidase matriptase staining with immunohistochemical detection of prostasin. We report that prostasin displays a near‐ubiquitous co‐localization with its candidate activator matriptase in a variety of normal epithelial tissues. These include simple, stratified, and pseudo‐stratified epithelium of the integumentary system, digestive tract, respiratory tract, and urogenital tract. However, matriptase and prostasin expression segregates during epithelial multi‐stage carcinogenesis to eventually become localized in separate compartments of the tumor. These data suggest that a matriptase‐prostasin zymogen activation cascade may be functionally operative in multiple epithelial tissues, but matriptase promotes epithelial carcinogenesis independent of prostasin. J. Cell. Physiol. 213: 237–245, 2007. © 2007 Wiley‐Liss, Inc.