bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urinary protein and coagulation‐fibrinolysis indicators in preeclampsia: Expression and significance
Authors: Chen Zhen , Fang Fang , Yu Xiaoqian 
Published: March 2024

AbstractThis study investigates the expression and significance of urinary protein and coagulation‐fibrinolysis indicators in preeclampsia, categorized into mild preeclampsia (109 cases) and severe preeclampsia (97 cases) based on disease severity. Additionally, 110 patients with gestational hypertension (gestational hypertension group) were included for comparative analysis. General information, laboratory indicators, urinary protein, and coagulation‐fibrinolysis indicator levels were collected for each group. Significant differences were observed in blood pressure among groups (P < .05), while uric acid, serum creatinine, aspartate transaminase, alanine transaminase, and triglycerides showed no significant differences (P > .05). Total cholesterol, triglycerides, and low‐density Lipoprotein levels in severe preeclampsia were higher than those in mild preeclampsia and gestational hypertension groups, whereas high‐density lipoprotein, albumin, and platelet levels were lower in severe preeclampsia. No significant differences were observed in prothrombin time or D‐dimer levels among groups (P > .05). Urinary protein, urinary protein quantification, activated partial thromboplastin time, thrombin time, and fibrinogen were identified as influencing factors for adverse maternal and infant outcomes in severe preeclampsia patients. The study concludes that urinary protein and coagulation‐fibrinolysis indicators are elevated in preeclampsia, particularly in severe preeclampsia cases, suggesting their potential use as diagnostic influencing factors for severe preeclampsia.
STRN3 promotes tumour growth in hepatocellular carcinoma by inhibiting the hippo pathway
Authors: Zhu Jie , Tang Wenjia , Fang Peiqi , Wang Chong , Gu Meixiu , Yang Wenjing , Pan Baishen , Wang Beili , Guo Wei 
Published: March 2024

AbstractHCC is a globally high‐incidence malignant tumour, and its pathogenesis is still unclear. Recently, STRN3 has been found to be elevated in various tumours, but its expression and biological functions in HCC have not been studied. In the study, clinical correlation analysis was performed on 371 liver cancer patients from TCGA database and liver cancer tissues and normal tissues from the GEO database. qRT‐PCR and western blotting were used to detect relevant proteins in cells, and CCK8 and colony formation experiments were performed to analyse cell proliferation ability. Transwell and wound healing experiments were performed to detect cell invasion ability, and flow cytometry was used to detect cell apoptosis. Single‐cell sequencing data and multiple immunofluorescence were analysed for the expression abundance and distribution of certain proteins. Immunohistochemistry was used to assess the expression of STRN3 in patients' tumour and adjacent non‐cancerous tissues. The results indicated STRN3 was highly expressed in liver tumour tissues and was closely associated with poor prognosis. Knockdown of STRN3 could significantly inhibit cell proliferation and migration ability. At the same time, we found that STRN3 could inhibit the Hippo pathway and promote the entry of YAP protein into the nucleus. Our study first found that STRN3 could promote tumour growth by inhibiting the Hippo pathway. The study of STRN3 can promote the understanding and treatment of the occurrence and development of HCC.
Identification of key regulatory molecules in the early development stage of Alzheimer's disease
Authors: Huang Bin , Ou Guan‐yong , Zhang Ni 
Published: March 2024

AbstractAlzheimer's disease (AD) is one of the most common neurodegenerative diseases, the incidence of which increases with age, and the pathological changes in the brain are irreversible. Recent studies have highlighted the essential role of long noncoding RNAs (lncRNAs) in AD by acting as competing endogenous RNAs (ceRNAs). Our aim was to construct lncRNA‐associated ceRNA regulatory networks composed of potential biomarkers for the early stage of AD. AD related datasets come from AlzData and GEO databases. The R package ‘Limma’ identifies differentially expressed genes (DEGs), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases for functional enrichment analysis. Protein–protein interactions (PPIs) in DEGs were constructed in the STRING database, and Cytoscape software identified DEGs. Convergent functional genomics (CFG) analysis of differentially expressed hub genes (referred to as early‐DEGs) in the brain before the development of AD pathology. The AlzData database analyses the expression levels of early‐DEGs in different nerve cells. The lncRNA‐miRNA‐mRNA regulatory network was established according to the ceRNA hypothesis. We identified four lncRNAs (XIST, NEAT1, KCNQ1OT1 and HCG18) and four miRNAs (hsa‐let‐7c‐5p, hsa‐miR‐107, hsa‐miR‐129‐2‐3p and hsa‐miR‐214‐3p) were preliminarily identified as potential biomarkers for early AD, competitively regulating Atp6v0b, Atp6v1e1 Atp6v1f and Syt1. This study indicates that NEAT1, XIST, HCG18 and KCNQ1OT1 act as ceRNAs in competitive binding with miRNAs to regulate the expression of Atp6v0b, Atp6v1e1, Atp6v1f and Syt1 before the occurrence of pathological changes in AD.
Identification of crosstalk genes relating to ECM‐receptor interaction genes in MASH and DN using bioinformatics and machine learning
Authors: Chen Chao , He Yuxi , Ni Ying , Tang Zhanming , Zhang Wensheng 
Published: March 2024

AbstractThis study aimed to identify genes shared by metabolic dysfunction‐associated fatty liver disease (MASH) and diabetic nephropathy (DN) and the effect of extracellular matrix (ECM) receptor interaction genes on them. Datasets with MASH and DN were downloaded from the Gene Expression Omnibus (GEO) database. Pearson's coefficients assessed the correlation between ECM‐receptor interaction genes and cross talk genes. The coexpression network of co‐expression pairs (CP) genes was integrated with its protein–protein interaction (PPI) network, and machine learning was employed to identify essential disease‐representing genes. Finally, immuno‐penetration analysis was performed on the MASH and DN gene datasets using the CIBERSORT algorithm to evaluate the plausibility of these genes in diseases. We found 19 key CP genes. Fos proto‐oncogene (FOS), belonging to the IL‐17 signalling pathway, showed greater centrality PPI network; Hyaluronan Mediated Motility Receptor (HMMR), belonging to ECM‐receptor interaction genes, showed most critical in the co‐expression network map of 19 CP genes; Forkhead Box C1 (FOXC1), like FOS, showed a high ability to predict disease in XGBoost analysis. Further immune infiltration showed a clear positive correlation between FOS/FOXC1 and mast cells that secrete IL‐17 during inflammation. Combining the results of previous studies, we suggest a FOS/FOXC1/HMMR regulatory axis in MASH and DN may be associated with mast cells in the acting IL‐17 signalling pathway. Extracellular HMMR may regulate the IL‐17 pathway represented by FOS through the Mitogen‐Activated Protein Kinase 1 (ERK) or PI3K‐Akt–mTOR pathway. HMMR may serve as a signalling carrier between MASH and DN and could be targeted for therapeutic development.
Modification effect of receipt of diabetes care on the association between COVID‐19 infection and HbA1c level during the first year of the coronavirus pandemic using a nationwide population‐based database
Authors: Okada Akira , Yamaguchi Satoko , Ono Sachiko , Kurakawa Kayo Ikeda , Yasunaga Hideo , Nangaku Masaomi , Yamauchi Toshimasa , Kadowaki Takashi 
Published: March 2024

ABSTRACTAims/IntroductionWe assessed the modification effect of adherence to diabetes care on the association between HbA1c levels and the risk of coronavirus disease 2019 (COVID‐19) among individuals with diabetes using a population‐based database.Material and MethodsWe retrospectively identified individuals with diabetes during routine health checkups performed in 2019 in Japan using a population‐based claims database (JMDC, Tokyo, Japan). We assessed the risk of COVID‐19 infection in 2020 in relation to HbA1c levels during routine checkups, stratified by the presence/absence of follow‐up for diabetes care in 2019. Several sensitivity analyses were performed.ResultsWe identified 65,956 individuals with an HbA1c ≥6.5% and fasting glucose ≥126 mg/dL in routine checkups, including 52,637 and 13,319 with and without at least one physician consultation for diabetes care in 2019, respectively. Although high HbA1c levels were associated with an increased risk of COVID‐19 infection in a dose‐dependent manner among individuals without diabetes care in 2019 (odds ratios, 1.53 and 2.17 in individuals with HbA1c of 7.0–7.9% and ≥8.0%, respectively) with a reference to HbA1c of 6.5–6.9%, individuals with diabetes care had no such trend in 2019 (odds ratios, 0.99 and 0.97 among individuals with HbA1c of 7.0–7.9% and ≥8.0%, respectively). Sensitivity analyses yielded consistent results when the variable definitions were changed and after multivariable adjustment with multiple imputation.ConclusionsThis population‐based study suggests that adherence to diabetes care may modify the association between HbA1c levels and the risk of COVID‐19 infection.
Research on the upgrade and maintenance method of apple soluble solids content models
Authors: He Cong , Jiang Xiaogang , Liu Yande 
Published: March 2024

AbstractIn this paper, 3 years (2020, 2021, and 2022) of Bingtangxin apples were used to study the upgrade and maintenance method of apple soluble solids content (SSC) model using near infrared (NIR) spectroscopy. Modeling by partial least squares (PLS) and one‐dimensional convolutional neural network (1D‐CNN) algorithm. The “base model” is built with apples in 2020 and then upgrade and maintain it. The upgraded model is used to predict the sample in 2021 and 2022. Three methods were used to upgrade and maintenance the model, which are based on updated data, direct correction‐based method, and similar band‐based method. Among the three upgrade methods, the model maintenance method using updated data had the greatest improvement in prediction correlation coefficients (Rp) in both years, and both obtained good predictions. The use of direct correction resulted in the greatest reduction in the root mean square error of prediction (RMSEP) in both years. However, the Rp of the model is not improved much by using the direct correction method. The model maintenance method using similar bands combined with different modeling methods also gives good prediction results. Finally, after comparison, the model maintenance method of selecting similar bands is preferred. In the case of limited improvement in the effect of using the similar band method, the model is maintained using the method of adding updated data. The investigation of model upgrade and maintenance methods can reduce human and material consumption and make apple SSC models more versatile.Practical applicationsNIR technology is the first choice for online fruit quality inspection, but its inspection process requires constant upgrading and maintenance with models. The quality of apples varies from year to year, and the model built with samples from a single year does not work well in predicting samples from other years, resulting in the need to upgrade and maintain the fruit quality detection model. However, sometimes, although a large amount of experimental material is lost, it still results in a generic calibration model that is too cumbersome and leads to poor prediction results. At this time, finding an effective model upgrade and maintenance method can both reduce the sample consumption and make the model prediction accuracy improve, and also reduce the model complexity and make the maintained model more robust.
Nutrition employability and graduate readiness: The Australian Working in Nutrition study
Authors: Clark Katya , Biesiekierski Jessica R. , Farrer Olivia D. , Stefoska‐Needham Anita , Beckett Emma L. , Lawlis Tanya , Mantzioris Evangeline , Swanepoel Libby 
Published: March 2024

AbstractBackgroundNutrition science graduates contribute to the nutrition workforce by bringing specialist knowledge and skills needed to address future food challenges. This study aims to provide a snapshot of the current employment landscape for nutrition science graduates in Australia and how well their degrees prepare them for employment.MethodA cross‐sectional survey of Australian tertiary nutrition graduates was conducted to explore tertiary training, employment pathways and their perceived preparedness for practice.ResultsThis study included a final sample of 119 graduates from 17 Australian tertiary institutions. Almost two‐thirds of respondents had completed further training. Most graduates (77%, n = 91) had worked in a food, nutrition science or health‐related role after their degree; the most frequently cited employment settings were government or public health organisations; research, not‐for‐profit or nongovernment organisations; and the food industry. Work‐integrated learning was identified as a key predictor of graduates working in a role that differed from their expectations as a student. The skill categories developed during nutrition training that were most valued in the workplace included nutrition and scientific knowledge, and professional and communication skills.ConclusionsThis study offers first insights into the current employment landscape for nutrition graduates across Australia. Findings show that current nutrition science professionals are highly qualified and prepared to navigate the evolving demands of nutrition practice. Regular review of graduate employment will inform nutrition science curriculum to enable graduates to be well equipped in the face of dynamic practice settings.
How does my partner see me? Metaperceptions of personality and couple satisfaction
Authors: Matera Camilla , Pirani Elena 
Published: March 2024

AbstractThis study examined the association between congruence in the two partners' perceptions and their relationship satisfaction. We considered congruence between metaperception and partner‐perception (meta‐partner) and between metaperceptions and self‐rated personality (meta‐self). The participants were 236 heterosexual couples. Each couple member reported couple satisfaction and personality traits (in terms of self‐perception, partner‐perception, and metaperception). The actor–partner interdependence model (APIM) revealed that individuals who believed to be viewed by their partner as more agreeable, conscientious, or emotionally stable than their partner saw them, were less likely to have a partner who was satisfied with the relationship. When metaperceptions of “Agreeableness” were higher than self‐perceptions, relationship satisfaction for both the actor and the partner was higher. These findings may help to guide therapeutic and clinical interventions aimed at promoting relationship satisfaction in the context of family and couple therapy.
Unmoving and uninflamed: Characterizing neuroinflammatory dysfunction in the Wistar‐Kyoto rat model of depression
Authors: Farinha‐Ferreira Miguel , Magalhães Daniela M. , Neuparth‐Sottomayor Mariana , Rafael Hugo , Miranda‐Lourenço Catarina , Sebastião Ana M. 
Published: March 2024

AbstractReductionistic research on depressive disorders has been hampered by the limitations of animal models. Recently, it has been hypothesized that neuroinflammation is a key player in depressive disorders. The Wistar‐Kyoto (WKY) rat is an often‐used animal model of depression, but no information so far exists on its neuroinflammatory profile. As such, we compared male young adult WKY rats to Wistar (WS) controls, with regard to both behavioral performance and brain levels of key neuroinflammatory markers. We first assessed anxiety‐ and depression‐like behaviors in a battery consisting of the Elevated Plus Maze (EPM), the Novelty Suppressed Feeding (NSFT), Open Field (OFT), Social Interaction (SIT), Forced Swim (FST), Sucrose Preference (SPT), and Splash tests (ST). We found that WKY rats displayed increased NSFT feeding latency, decreased OFT center zone permanence, decreased EPM open arm permanence, decreased SIT interaction time, and increased immobility in the FST. However, WKY rats also evidenced marked hypolocomotion, which is likely to confound performance in such tests. Interestingly, WKY rats performed similarly, or even above, to WS levels in the SPT and ST, in which altered locomotion is not a significant confound. In a separate cohort, we assessed prefrontal cortex (PFC), hippocampus and amygdala levels of markers of astrocytic (GFAP, S100A10) and microglial (Iba1, CD86, Ym1) activation status, as well as of three key proinflammatory cytokines (IL‐1β, IL‐6, TNF‐α). There were no significant differences between strains in any of these markers, in any of the regions assessed. Overall, results highlight that behavioral data obtained with WKY rats as a model of depression must be carefully interpreted, considering the marked locomotor activity deficits displayed. Furthermore, our data suggest that, despite WKY rats replicating many depression‐associated neurobiological alterations, as shown by others, this is not the case for neuroinflammation‐related alterations, thus representing a novel limitation of this model.image
Development of a functional outcome measure for riboflavin transporter deficiency
Authors: Fennessy Jack R. , Donlevy Gabrielle A. , McKay Marnee J. , Burns Joshua , Cornett Kayla M. D. , Menezes Manoj P. 
Published: March 2024

AbstractBackground and AimsRiboflavin transporter deficiency (RTD) is a progressive inherited neuropathy of childhood onset, characterised clinically by pontobulbar palsy, sensory ataxia, sensorineural deafness, muscle weakness, optic atrophy and respiratory failure. A robust and responsive functional outcome measure is essential for future clinical trials of disease‐modifying therapies including genetic therapies. The Charcot–Marie–Tooth disease Pediatric Scale (CMTPedS) is a well‐validated outcome measure for CMT and related neuropathies, and might have utility for measuring disease progression in individuals with RTD. However, the CMTPedS requires modifications to account for phenotypic differences between children with CMT and RTD. The aim of this study was to develop a functional outcome measure based on the CMTPedS for specific use in individuals with RTD.MethodsThe CMTPedS data collected over the last 10 years in individuals with RTD attending the Peripheral Neuropathy Management Clinic at the Children's Hospital at Westmead (Sydney, Australia) were reviewed to evaluate each item within the CMTPedS. A literature review of articles published until September 2021 for functional outcome measures generated an item pool for pilot testing. The results of this pilot testing, alongside analysis of existing CMTPedS item scores in the RTD cohort, informed the modification of the CMTPedS.ResultsCMTPedS data were reviewed for eight individuals over the past 10 years. Two items were identified as requiring modification or removal and additional items of proximal strength and function needed to be considered. Six studies were identified in the literature review, and five items were selected for pilot testing. ‘Shoulder internal rotation’ and the ‘30‐s sit to stand test’ were added as proximal measures of strength and function. The composite balance item comprising nine tasks in the CMTPedS showed a ceiling effect and was replaced with the single ‘Feet apart on a line eyes open’ balance item. ‘Pinprick sensation’ was removed due to a floor effect.InterpretationThis study provides preliminary evidence that the Riboflavin Transporter Deficiency Pediatric Scale (RTDPedS) is a functional outcome measure covering strength, upper and lower limb function, balance and mobility for individuals with RTD to assess disease severity and progression in clinical trials and cohort studies.
Higher percentage of CD34+ stem cells and elevated efficacy in androgenetic alopecia treatment observed in CGF prepared from 640 nm laser‐pretreated blood: A preliminary study
Authors: Gao Jin , Xiao Qin , Lu Yongzhou , Guo Jing , Luan Dongdong , Jia Chuanlong , Xu Qiannan , Xu Nan 
Published: March 2024

AbstractBackgroundConcentrated growth factor (CGF) injection has proven effective in treating androgenetic alopecia (AGA). The primary mechanism of CGF in treating AGA is thought to be the CD34+ stem cells and platelets‐associated growth factors being injected into the scalp. CGF efficacy in treating AGA may rely on the activation level of these stem cells and platelets. The 640 nm laser is a United States Food and Drug Administration approved AGA treatment that activates follicle stem cells. Therefore, we hypothesize that pretreating CGF with a 640 nm laser may further activate CD34+ stem cells and platelets, thereby improving the efficacy of CGF in treating AGA.ObjectiveThis study aims to investigate whether 640 nm laser pretreated CGF (640CGF) has a greater effect in treating AGA than 640 nm laser non‐pretreated CGF (N640CGF) and evaluate whether 640 nm laser pretreatment changed CD34+ cell percentage.MethodsThis study enrolled 10 patients (8 male, 2 female) with AGA aged 18–60 years who received CGF injections. The 640CGF group was pretreated with a 640 nm laser at an energy density of 4 J/cm2, with a 30 cm irradiation distance for 30 min. Half of the scalp was treated with 640CGF, whereas the other half was treated with N640CGF. The injection was prepared by a doctor who did not know which blood tube had been pretreated. The treatment efficacy was evaluated using a trichoscope 1 month after injection.ResultsAll 10 (100%) patients participated in the follow‐up visit, and a higher quantity of new hairs was observed on the side injected with 640CGF than N640CGF (p = 0.019). Additionally, fewer malnourished hairs were observed on the 640CGF pretreated side (p = 0.015). No serious adverse events were reported.ConclusionsA higher percentage of CD34+ stem cells and improved efficacy in AGA treatment could be observed with CGF prepared from 640 nm laser‐pretreated blood.
Adipose stem cell‐derived exosomes promote wound healing by regulating the let‐7i‐5p/GAS7 axis
Authors: Liu Xiaosong , Wang Biao 
Published: March 2024

AbstractBackgroundInjury to skin tissue is devastating for human health, making it imperative to devise strategies for hastening wound healing. Normal wound healing is a complex process comprising overlapping steps, including hemostasis, inflammatory response, proliferation, and matrix remodeling. This study investigated the effects of adipose stem cell‐derived exosomes (ADSC‐exos) on wound healing and the underlying mechanisms.MethodsIn vitro hydrogen peroxide (H2O2)‐treated human keratinocyte (HaCaT) cell lines and in vivo animal wound models were established for this purpose. The cell migration was assessed using transwell and wound healing assays, while exosome biomarker expressions were studied using western blot. Moreover, adipose stem cells were identified using flow cytometry, alizarin red S and oil red O staining, and transmission electron microscopy.ResultsResults indicated that H2O2 treatment inhibited the cell viability and migration of HaCaT cells while being promoted by ADSC‐exos. Mechanistic investigations revealed that microRNA‐let‐7i‐5p (let‐7i‐5p) in ADSC‐exos was carried into the HaCaT cells, inhibiting the expression of growth arrest‐specific‐7 (GAS7). Rescue experiments further verified these results, which indicated that GAS7 overexpression reversed the effect of let‐7i‐5p on the viability and migration of HaCaT cells, suggesting ADSC‐exos promoted wound healing via the let‐7i‐5p/GAS7 axis.ConclusionAdipose stem cell‐derived‐exos enhanced the viability and migration of HaCaT via carrying let‐7i‐5p and targeting GAS7, ultimately promoting wound healing in rats.