bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hematopoietic stem cell differentiation affects expression and function of MRP4 (ABCC4), a transport protein for signaling molecules and drugs
Authors: Oevermann Lena , Scheitz Jan , Starke Katharina , Köck Kathleen , Kiefer Thomas , Dölken Gottfried , Nießen Juliane , Greinacher Andreas , Siegmund Werner , Zygmunt Marek , Kroemer Heyo K. , Jedlitschky Gabriele , Ritter Christoph A. 
Published: December 2008

AbstractSeveral types of peripheral blood cells express ABC transporters. ABCC4 (MRP4) and ABCC5 (MRP5) localize to different cellular sites and fulfill lineage‐specific functions such as mediator storage in platelets' dense granules. All mature blood cells originate from the same precursor and specific functionalities arise during differentiation. To characterize this process, expression, localization and function of MRP4 and MRP5 were assessed in differentiating human CD34+ progenitors and leukemia cell lines using real time polymerase chain reaction (PCR), immunofluorescence microscopy and cell viability assays. Median MRP4 mRNA copy numbers were significantly enhanced by megakaryocytic differentiation from 7.9 × 103 to 5.8 × 104 copies per nanograms of total RNA (p < 0.05) in CD34+ progenitors and in M‐07e cells (MRP4 mRNA/18S rRNA ratios: 5.4 ± 3.8 × 10−4 vs. 2.7 ± 0.9 × 10−3 for native and differentiated cells, respectively, p < 0.05), and MRP4 protein was localized to granular structures and to the plasma membrane both in differentiated progenitors and bone marrow megakaryocytes. In contrast, expression of MRP4 decreased during maturation to leukocytes (MRP4 mRNA/18S rRNA ratios: 5.2 × 10−3 for native vs. 3.5 × 10−3 for CD34+ cells in the presence of G‐CSF, p < 0.05) and was significantly reduced in mature monocytes and granulocytes compared with progenitors (MRP4 mRNA/18S rRNA ratios: 8.1 ± 5.4 × 10−5 and 2.8 ± 1.6 × 10−4 vs. 1.2 ± 0.7 × 10−3, respectively, p < 0.05). Expression of MRP5 was not significantly altered under all differentiation conditions. These results indicate that MRP4 expression is differentially regulated during hematopoiesis. The increase of MRP4 together with its specific localization during differentiation toward megakaryocytes supports the concept of platelet specific functions whereas decreased transporter expression in leukocyte differentiation may have implications for chemotherapy. © 2008 Wiley‐Liss, Inc.
All‐trans‐retinoic acid alters Smads expression in embryonic neural tissue of mice
Authors: Zhang Juntao , Li Rong , He Quanren , Li Wan‐I. , Niu Bo , Cheng Niuliang , Zhou Ran , Zhang Ting , Zheng Xiaoying , Xie Jun 
Published: December 2008

AbstractRetinoic acid can cause malformations of the developing nervous system. Smad signaling is involved in embryonic development. The current study investigated all‐trans‐retinoic acid (ATRA)‐induced alteration of Smad expression in the developing neural tubes of mice. Pregnant mice were treated with a single dose of 50 mg/kg ATRA by oral gavage on embryonic day (E) 7. Western immunoblotting was used to examine Smads proteins, particularly phosphorylated (p‐) Smad1, total Smad1 and Smad6 in the neural tissue of the embryos on E8–E11 following treatment. Results showed that ATRA treatment significantly increased expression of both p‐Smad1 and total Smad1, while Smad6 was decreased in neural tissues of ATRA‐exposed embryos in utero from E8 to E11, a critical period for neural tube formation. Data suggest that disruption of Smad signaling may be involved in ATRA‐induced neural tube defects. Copyright © 2008 John Wiley & Sons, Ltd.
Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposures
Authors: Fırat Özgür , Çogun Hikmet Y. , Aslanyavrusu Sabahattin , Kargın Ferit 
Published: December 2008

AbstractWe investigated the effects of Zn, Cd and a Zn + Cd mixture on antioxidant parameters and metal accumulation in Oreochromis niloticus. Fish were exposed to 0.5 and 5.0 mg l−1 Zn, 0.1 and 1.0 mg l−1 Cd, and 0.5 mg l−1 Zn + 0.1 mg l−1 Cd and 5.0 mg l−1 Zn + 1.0 mg l−1 Cd mixtures for 7 and 28 days to determine Zn and Cd accumulation, reduced glutathione (GSH) level and glucose‐6‐phosphate dehydrogenase (G6PD) activity in gill and liver. There was increasing accumulation of the metals in the tissues with increasing concentrations of metals in the exposure medium and with increasing duration of exposure (except at the lower concentration of Zn). Concentration of metals in the tissues of fish exposed to the Zn + Cd combination were significantly lower than in fish exposed to the single metal. The highest metal accumulation was observed in the liver. Exposure to the heavy metals affected the antioxidant parameters in the tissues, with both GSH level and G6PD activity in the gill and liver being increased under Zn, Cd and Zn + Cd exposures, especially in their higher concentrations. These increases in the antioxidant responses were higher with the Cd alone, and in combination with Zn, than with Zn alone. Furthermore, GSH level and G6PD activity increased with increasing exposure period only for Cd alone, and in Cd combination with Zn. The results indicate that O. niloticus resisted oxidative stress induced by heavy metal exposure by antioxidant mechanisms. Copyright © 2008 John Wiley & Sons, Ltd.
Capillary GC using pyridyl β‐cyclodextrin stationary phase
Authors: Shen Gangyi , Cui Jian , Yang Xinling , Ling Yun 
Published: December 2008

AbstractA new β‐CD derivative, heptakis [2,6‐di‐O‐pentyl‐3‐O‐(4′‐chloro‐5′‐pyridylmethyl)]‐β‐CD, was synthesized by the selective introduction of a pyridyl group on the 3‐positions of β‐CD. The chromatographic properties of the pyridyl β‐CD derivative were studied by using it as the stationary phase in capillary GC. The polarity of the prepared stationary phase was moderate, and the separation results demonstrated that the prepared stationary phase possessed excellent separation ability and chiral recognition for a wide range of analytes. Not only the aromatic positional isomers, such as o‐, m‐, p‐xylene and α‐, β‐naphthol isomers, but also some compounds with multi‐stereogenic centers, such as n‐(1‐methylpropyl)‐3‐(2,2‐dichloroethenyl)‐2,2‐dimethylcyclopropanecarboxamide and n‐(1‐methylpropyl)‐3‐(2‐chloro‐3,3,3‐trifluoropropenyl)‐2,2‐dimethylcyclopropanecarboxamide with three stereogenic centers including eight configurational isomers, were successfully separated. The results also indicated that the polarity of the β‐CD derivative, and the hydrogen bonding between the β‐CD derivative, and the analytes had a very important effect on separation.
Predictions of large stress reversals in true triaxial tests on cross‐anisotropic sand
Authors: Gutta Suresh K. , Yamamuro Jerry A. , Lade Poul V. 
Published: December 2008

AbstractA rotational kinematic hardening constitutive model with the capability of predicting the behavior of soil during three‐dimensional stress reversals has been developed. An existing elasto‐plastic constitutive model, the Single Hardening Model, utilizing isotropic hardening serves as the basic framework in these formulations. The framework of the kinematic hardening model was discussed in a companion paper. The previously proposed cross‐anisotropic Single Hardening Model is added to the present kinematic hardening mechanism to capture inherent anisotropy of sands in addition to the stress reversals. This model involves 13 parameters, which can be determined from simple laboratory experiments, such as isotropic compression, drained triaxial compression and triaxial extension tests. The results from a series of true triaxial tests with large three‐dimensional stress reversals performed on medium dense cross‐anisotropic Santa Monica Beach sand are employed for comparison with predictions. Copyright © 2008 John Wiley & Sons, Ltd.
Heart rate variability: An objective measure of autonomic activity and bladder sensations during urodynamics
Authors: Mehnert Ulrich , Knapp Peter A. , Mueller Nicole , Reitz André , Schurch Brigitte 
Published: December 2008

AbstractAimsThere are still controversies regarding the reproducibility of cystometric data and no objective measurement of bladder sensations is currently available. Additionally, very little information exists about autonomic activity during standard filling cystometry (FC). The aim of this study was to evaluate the use of heart rate variability (HRV) analysis as a reliable monitor of the autonomic nervous system and objective measure for bladder sensations during FC.MethodsIn a volunteer sample of healthy female subjects a standard FC at 25 ml/min was performed, using an 8 Fr microtip catheter with integrated pressure transducers. During FC, subjects had to indicate first sensation of filling (FSF), first desire to void (FDV) and strong desire to void (SDV). A 3‐lead electrocardiogram was continuously recorded. After 5 h all measurements were repeated. Power spectrum analysis was used to analyse HRV, to obtain low frequency (LF) and high frequency (HF) parameters, from which the LF/HF ratio was derived.Results12 subjects with a mean age of 23.3 ± 2.3 years could be included. 11 of 12 subjects completed both measurement sessions. One subjects had to be excluded, due to irritating urethral discomfort following catheterisation. The LF/HF ratio showed a reproducible activation pattern in the healthy subjects with a stable sympathovagal balance until FDV. Before SDV was indicated, the sympathovagal balance started to shift towards sympathetic activation and caused a significant increase in LF/HF.ConclusionHRV analysis seems to be a useful indicator for the general activation pattern of the sympathovagal balance during FC, correlating the intensity of the bladder filling sensation to stress and sympathetic activation. Neurourol. Urodynam. 28:313–319, 2009. © 2008 Wiley‐Liss, Inc.
Intracranial hemorrhage in childhood immune thrombocytopenic purpura
Authors: Choudhary Dharma R. , Naithani Rahul , Mahapatra M. , Kumar Rajat , Mishra Pravas , Saxena Renu 
Published: December 2008

AbstractWe retrospectively analyzed 750 patients with ITP for development of intracranial hemorrhage (ICH). Seventeen cases with age range of 10 months to 18 years were studied. Ten patients were of acute ITP and seven had chronic ITP. Nine patients developed ICH one month after the onset of ITP and five patients had ICH on presentation. ICH was precipitated by trauma in four patients and possibly the use of NSAIDs in one patient. Median platelets counts at the time of ICH were 12 × 109/L (range 2–50 × 109/L). Most patients were treated with corticosteroids. Four patients (24%) died due to ICH. Pediatr Blood Cancer 2009;52:529–531. © 2008 Wiley‐Liss, Inc.
Fatal hemophagocytic lymphohistiocytosis in X‐linked chronic granulomatous disease associated with a perforin gene variant
Authors: van Montfrans Joris M. , Rudd Eva , van de Corput Lisette , Henter Jan‐Inge , Nikkels Peter , Wulffraat Nico , Boelens Jaap J. 
Published: December 2008

AbstractA patient with previously unrecognized X‐linked chronic granulomatous disease (X‐CGD) died of multi‐organ failure, secondary to ongoing infection and hemophagocytic lymphohistiocytosis (HLH). Post mortem histological investigations were compatible with X‐CGD, and a CYBB gene mutation was confirmed. No homozygous mutations in the genes encoding perforin (PRF1), MUNC 13‐4 or syntaxin‐11 (STX11) were found; however, there was a heterozygous alteration c.1471G>A in the PRF1 gene causing a p.Asp491Asn substitution. Although this substitution has not been reported to cause primary or secondary HLH, we speculate that it may have made the patient more susceptible for HLH under the circumstances of ongoing infection associated with X‐CGD. Pediatr Blood Cancer 2009;52:527–529. © 2008 Wiley‐Liss, Inc.
Systematic comparison of the human saliva and plasma proteomes
Authors: Yan Weihong , Apweiler Rolf , Balgley Brian M. , Boontheung Pinmanee , Bundy Jonathan L. , Cargile Benjamin J. , Cole Steve , Fang Xueping , Gonzalez‐Begne Mireya , Griffin Timothy J. , Hagen Fred , Hu Shen , Wolinsky Lawrence E. , Lee Cheng S. , Malamud Daniel , Melvin James E. , Menon Rajasree , Mueller Michael , Qiao Renli , Rhodus Nelson L. , Sevinsky Joel R. , States David , Stephenson James L. , Than Shawn , Yates John R. , Yu Weixia , Xie Hongwei , Xie Yongming , Omenn Gilbert S. , Loo Joseph A. , Wong David T. 
Published: December 2008

AbstractThe proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study to comprehensively identify and catalog the human ductal salivary proteome led to the compilation of 1166 proteins. The protein complexity of both saliva and plasma is large, suggesting that a comparison of these two proteomes will provide valuable insight into their physiological significance and an understanding of the unique and overlapping disease diagnostic potential that each fluid provides. To create a more comprehensive catalog of human salivary proteins, we have first compiled an extensive list of proteins from whole saliva (WS) identified through MS experiments. The WS list is thereafter combined with the proteins identified from the ductal parotid, and submandibular and sublingual (parotid/SMSL) salivas. In parallel, a core dataset of the human plasma proteome with 3020 protein identifications was recently released. A total of 1939 nonredundant salivary proteins were compiled from a total of 19 474 unique peptide sequences identified from whole and ductal salivas; 740 out of the total 1939 salivary proteins were identified in both whole and ductal saliva. A total of 597 of the salivary proteins have been observed in plasma. Gene ontology (GO) analysis showed similarities in the distributions of the saliva and plasma proteomes with regard to cellular localization, biological processes, and molecular function, but revealed differences which may be related to the different physiological functions of saliva and plasma. The comprehensive catalog of the salivary proteome and its comparison to the plasma proteome provides insights useful for future study, such as exploration of potential biomarkers for disease diagnostics.
Evidence of carrier leakage into the L‐valley in InAs‐based quantum cascade lasers under high hydrostatic pressure
Authors: Marko Igor P. , Adams Alfred R. , Sweeney Stephen J. , Teissier Roland , Baranov Alexei N. , Tomić Stanko 
Published: December 2008

AbstractTo investigate carrier scattering processes in short wavelength InAs/AlSb quantum cascade lasers we carried out experimental and theoretical studies of the threshold current, Ith, as a function of high hydrostatic pressure and temperature. Using the calculated pressure dependence of the optical phonon scattering current, Iph, and the estimated pressure dependence of leakage current, Ileak, we show that carrier leakage from the upper laser levels into the indirect L‐valley of the conduction band in InAs quantum wells is negligible in the 3.3 μm QCLs at RT leading to their superior temperature performance. In the shorter wavelength devices emitting at 2.9 μm, this loss mechanism is more important and accounts for up to 13% of Ith at 190 K. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Transverse velocity distributions in channels with emergent bank vegetation
Authors: Hirschowitz P. M. , James C. S. 
Published: December 2008

AbstractDefining hydraulic habitat conditions in rivers requires prediction of local flow velocities, which are strongly influenced by the presence of emergent vegetation along the banks. Previously proposed methods for describing the transverse variation of depth‐averaged velocity away from the vegetation edge are shown by comparison with independent laboratory data to be inadequate. One of the methods is modified on the basis of new data to provide realistic descriptions over a wide range of conditions. The new method enables prediction of the maximum velocity in both wide and relatively narrow channels, the apparent shear stress at the vegetation interface, and the variation of velocity from the vegetation boundary to the point where the maximum velocity occurs. Copyright © 2008 John Wiley & Sons, Ltd.
Electronic‐Type‐ and Diameter‐Dependent Reduction of Single‐Walled Carbon Nanotubes Induced by Adsorption of Electron‐Donor Molecules
Authors: Zhou Jing , Maeda Yutaka , Lu Jing , Tashiro Atsushi , Hasegawa Tadashi , Luo Guangfu , Wang Lu , Lai Lin , Akasaka Takeshi , Nagase Shigeru , Gao Zhengxiang , Qin Rui , Mei Wai Ning , Li Guangping , Yu Dapeng 
Published: December 2008

AbstractThe adsorption of the organic donor molecules tetrakis(dimethylamino)ethylene (TDAE) and cobaltocene (CoCp2) on high‐pressure CO decomposition (HiPco) single‐walled carbon nanotubes (SWNTs) is investigated using density functional theory (DFT), optical absorption, and Raman spectra methods. The selective reduction of SWNTs according to the electronic type and diameter of SWNTs is revealed. The reduction rate decreases in the order: metallic SWNTs ≥ large‐diameter semiconducting SWNTs > small‐diameter semiconducting SWNTs.
Glycoprofiling of the Human Salivary Proteome
Authors: Sondej Melissa , Denny Patricia A. , Xie Yongming , Ramachandran Prasanna , Si Yan , Takashima Jona , Shi Wenyuan , Wong David T. , Loo Joseph A. , Denny Paul C. 
Published: December 2008

AbstractIntroductionGlycosylation is an important component for a number of biological processes and is perhaps the most abundant and complicated of the known post-translational modifications found on proteins.MethodsThis work combines two-dimensional (2-D) polyacrylamide gel electrophoresis and lectin blotting to map the salivary glycome and mass spectrometry to identity the proteins that are associated with the glycome map. A panel of 15 lectins that recognize six sugar-specific categories was used to visualize the type and extent of glycosylation in saliva from two healthy male individuals. Lectin blots were compared to 2-D gels stained either with Sypro Ruby (protein stain) or Pro-Q Emerald 488 (glycoprotein stain).ResultsEach lectin shows a distinct pattern, even those belonging to the same sugar-specific category. In addition, the glycosylation profiles generated from the lectin blots show that most salivary proteins are glycosylated and that the profiles are more widespread than is demonstrated by the glycoprotein-stained gel. Finally, the coreactivity between lectins was measured to determine what types of glycan structures are associated with one another and also the population variation of the lectin reactivity for 66 individuals were reported.ConclusionsThis starting 2-D gel glycosylation reference map shows that the scientifically accepted, individual oligosaccharide variability is not limited to a few large glycoproteins such as MUC5B, but are found on most members of the salivary proteome. Finally, in order to see the full range of oligosaccharide distribution, multiple reagents or lectins are needed.
The Nurse's Role in Managing Necrotizing Fasciitis
Authors: Magel Deborah C. 
Published: December 2008

ABSTRACTNECROTIZING FASCIITIS (NF), also known as “flesh‐eating disease,” is a rapid‐acting, potentially deadly infection.THIS CONDITION is devastating in its ability to spread through the body at an alarming rate. It can be caused by almost any type of injury that introduces microorganisms into the subcutaneous tissues.THE INITIAL ASSESSMENT can be deceptive, so awareness is the key to recognition. This condition often goes unrecognized until it is too late or major disfigurement results. Vital nursing strategies for managing the care of patients with NF include early recognition, accurate assessment, and aggressive treatment. AORN J 88 (December 2008) 977–982. © AORN, Inc, 2008.
Does Cuba Fit Yet or Is It Still ‘Exceptional’?
Authors: KAPCIA ANTONI 
Published: December 2008

AbstractMuch of the external literature on the Cuban Revolution has been characterised by two dichotomies between ‘exceptionalism’ on the hand and the application of non-Cuban paradigms on the other, and that between Fidel-focussed interpretations and more systemic perspectives. This article examines the evolution of these dichotomies, from initial enthusiasm or condemnation, through an emerging awareness of a historical dimension, a focus on the social revolution, and new disenchantments, to the emergence of a less polemical attention to detail before the post-1991 return to type. In the light of this trajectory, new approaches are suggested to break with these patterns, while acknowledging the challenges for exogenous research.
The Left in Transition: The Cuban Revolution in US Third World Politics
Authors: GRONBECK-TEDESCO JOHN A. 
Published: December 2008

AbstractThis article examines the ways in which Cuba's revolution shaped the changing politics of the Left in the United States. Using critical strategies of transnationalism, it illustrates how a dialogue developed between US activists and Cuban cultural producers, and reveals how Cuba's revolutionary discourse inflected the radical shift towards Third World nationalism. As the post-Bandung global moment brought a network of new political and cultural affiliations, Cuba's state apparatus invested in the manufacture and dissemination of tricontinental politics worldwide. This alternative moral and political imaginary lent authority to Havana's status as leader of the Third World and drew global attention to Cuba's revolutionary model in new thinking on post-colonial identity and culture. This new thinking imbibed the US Left's humanistic turn which challenged standard boundaries of race, class and nation. The dialectical nature of Cuban political discourse and social activism in the United States changed Cuban exigencies while it enticed US dissidents to experience the exception in the western hemisphere that fought for the greater moral good.
Survival of seeds in hypervelocity impacts
Authors: Jerling Aaron , Burchell Mark J. , Tepfer David 
Published: December 2008

AbstractPanspermia (‘seeds everywhere’) postulates that life naturally migrates through space. Laboratory studies of Panspermia often examine the survival of Earth's species under the conditions thought to occur during transfer through space. Much of this research has centred on bacteria, but here we consider seeds themselves. We simulated the extreme accelerations necessary for their hypothetical ejection from a planetary surface and the impacts associated with their arrival on another planet. Seeds of tobacco, alfalfa and cress were fired into water at speeds in the range 1–3 km s−1, corresponding to impact shock pressures of circa 0.24–2.4 GPa. No seeds remained intact and able to germinate, even at the lowest speeds. Although fragmentation occurred, even at 3 km s−1 the size of some of the fragments was about 25% that of the seeds. Thus, whilst the seeds themselves did not survive extreme shocks, a substantial fraction of their mass did and might successfully deliver complex organic materials after impact. These results are discussed with respect to ancient Panspermia and the potential of contemporary impacts to eject living organisms into space.