bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Tissue Factor Pathway Inhibitor in Tumor Growth and Metastasis
Authors: Amirkhosravi Ali , Meyer Todd , Amaya Mildred , Davila Monica , Mousa Shaker A. , Robson Theresa , Francis John L. 
Published: November 2007

AbstractClotting activation occurs frequently in cancer. Tissue factor (TF), the most potent initiator of coagulation, is expressed aberrantly in many types of malignancy and is involved not only in tumor-associated hypercoagulability but also in promoting tumor angiogenesis and metastasis via coagulation-dependent and coagulation-independent (signaling) mechanisms. Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis. Heparins and heparin derivatives, which induce the release of TFPI from the vascular endothelium, also exhibit antitumor effects, and TFPI may contribute significantly to those effects. Indeed, a non-anticoagulant low-molecular-weight heparin with intact TFPI-releasing capacity has been shown to have significant antimetastatic effect in a similar experimental mouse model. The evidence supporting the dual inhibitory functions on TF-driven coagulation and signaling strengthen the rationale for considering TFPI as a potential anticancer agent. This article primarily summarizes the evidence for antiangiogenic and antimetastatic effects of TFPI and describes its potential mechanisms of action. The possible application of TFPI and other inhibitors of TF as potential anticancer agents is described, and information regarding potential antitumor properties of TFPI-2 (which has structural similarities to TFPI) is also included.
Mental Health Care for our Ageing Australian Population
Authors: Hassett Anne , Fortune Tracy , Smith Brad 
Published: November 2007

Objective: In the context of the National Strategy for an Ageing Australia, the aim of this paper was to highlight some of the challenges for aged mental health services as our population ages. Conclusions: The demand for greater consumer participation in treatment planning and provision, and the increasing complexity of managing medical and psychiatric comorbidity in our ‘old-old’ population will be major drivers for improving our models of clinical practice and forging stronger partnerships between the public and private sectors.
Qualified therapists’ experience of personal therapy
Authors: Daw Becky , Joseph Stephen 
Published: November 2007

AbstractRecent work shows that the majority of therapists strongly value personal therapy for what it can bring to their professional practice, and for personal development. The aim of this study was to explore qualified therapists’ experiences of personal therapy. Of the 220 qualified therapists who were contacted via post and asked to take part in a questionnaire study, 48 returned the completed questionnaire, which consisted of a series of closed and open questions. Two‐thirds of respondents had engaged in personal therapy. The two most frequently cited reasons for engaging in personal therapy were personal growth and personal distress. Interpretative phenomenological analysis (IPA) identified two broad domains: impact on the person and impact on the professional. Results show that those surveyed recognised the value of personal therapy to themselves as a form of self‐care and personal development. Personal therapy was valued in professional practice as a form of experiential learning from being in the client role. The limitations of this research included a relatively low response rate, raising the question of whether those most likely to have had positive experiences of personal therapy were more likely to participate. Future research might deliberately seek to understand negative experiences of personal therapy. Other avenues for future research include the need to understand how experiences of therapy relate to gender, profession, and therapeutic orientation.
Introducing humanistic group counselling for somatisation in a primary care practice: Preliminary findings
Authors: Graham J. , Manor O. , Wiseman S. 
Published: November 2007

AbstractCan group counselling help patients who present with symptoms that cannot be explained medically? Preliminary results of working with one such group are reported. Six patients in a primary care practice were offered weekly sessions for half a year. The findings suggest that the patients attended all group sessions. The patients reported experiences known to be related to group counselling, and when the group ended, patient reports show that the severity of their problems was reduced significantly (p<.001). Furthermore, during the six months after the group ended patients significantly (p<.025) reduced their visits to their general practitioners. These results seem to call for wider use of humanistic group counselling with somatising patients and further research into the impact of such form of counselling.
Single session and walk‐in psychotherapy: A descriptive account of the literature
Authors: Cameron Christopher L. 
Published: November 2007

AbstractOver the last several decades single session psychotherapy has become a mainstream psychotherapeutic approach. A steadily increasing demand for psychotherapeutic services and a continuous reduction in mental health care resources worldwide are both largely responsible for this development. This paper describes single session psychotherapy and provides a brief review of the relevant literature. Single session psychotherapy delivered in walk‐in settings is also discussed. Therapists who are unfamiliar with single session psychotherapy will find that this paper provides a concise introduction to the essential processes and outcomes of this psychotherapeutic approach.
Assessment of measurement uncertainty via observation equations
Authors: Possolo Antonio , Toman Blaza 
Published: November 2007

According to theGuide to the Expression of Uncertainty in Measurement(GUM) (1995, Geneva, Switzerland: International Organization for Standardization (ISO)), the uncertainty in an estimate of the value of a measurand is assessed by propagating the uncertainty in estimates of values of input quantities, based on ameasurement equationthat expresses the former value as a known function of the latter values. However, in measurement situations where some of the input quantities in turn depend on the measurand, this approach is circuitous and ultimately impracticable.An alternative approach starts from theobservation equation, which relates the experimental data to the measurand: this allows a uniform treatment of the most diverse metrological problems, and, once it is used in the context of Bayesian inference, also facilitates the exploitation of any information that may pre-exist about the measurand, alongside the information that fresh experimental data provide about it.The widest applicability of the observation equation approach is illustrated with detailed examples concerning the lifetime of mechanical parts, the measurement of mass, the calibration of a non-linear model in biochemistry and the estimation of a consensus value for arsenic concentration in a sample measured by multiple laboratories.
A Bifunctional Locus (BIO3-BIO1) Required for Biotin Biosynthesis in Arabidopsis
Authors: Muralla Rosanna , Chen Elve , Sweeney Colleen , Gray Jennifer A. , Dickerman Allan , Nikolau Basil J. , Meinke David 
Published: November 2007

AbstractWe identify here the Arabidopsis (Arabidopsis thaliana) gene encoding the third enzyme in the biotin biosynthetic pathway, dethiobiotin synthetase (BIO3; At5g57600). This gene is positioned immediately upstream of BIO1, which is known to be associated with the second reaction in the pathway. Reverse genetic analysis demonstrates that bio3 insertion mutants have a similar phenotype to the bio1 and bio2 auxotrophs identified using forward genetic screens for arrested embryos rescued on enriched nutrient medium. Unexpectedly, bio3 and bio1 mutants define a single genetic complementation group. Reverse transcription-polymerase chain reaction analysis demonstrates that separate BIO3 and BIO1 transcripts and two different types of chimeric BIO3-BIO1 transcripts are produced. Consistent with genetic data, one of the fused transcripts is monocistronic and encodes a bifunctional fusion protein. A splice variant is bicistronic, with distinct but overlapping reading frames. The dual functionality of the monocistronic transcript was confirmed by complementing the orthologous auxotrophs of Escherichia coli (bioD and bioA). BIO3-BIO1 transcripts from other plants provide further evidence for differential splicing, existence of a fusion protein, and localization of both enzymatic reactions to mitochondria. In contrast to most biosynthetic enzymes in eukaryotes, which are encoded by genes dispersed throughout the genome, biotin biosynthesis in Arabidopsis provides an intriguing example of a bifunctional locus that catalyzes two sequential reactions in the same metabolic pathway. This complex locus exhibits several unusual features that distinguish it from biotin operons in bacteria and from other genes known to encode bifunctional enzymes in plants.
Diarch Symmetry of the Vascular Bundle in Arabidopsis Root Encompasses the Pericycle and Is Reflected in Distich Lateral Root Initiation
Authors: Parizot Boris , Laplaze Laurent , Ricaud Lilian , Boucheron-Dubuisson Elodie , Bayle Vincent , Bonke Martin , De Smet Ive , Poethig Scott R. , Helariutta Yka , Haseloff Jim , Chriqui Dominique , Beeckman Tom , Nussaume Laurent 
Published: November 2007

AbstractThe outer tissues of dicotyledonous plant roots (i.e. epidermis, cortex, and endodermis) are clearly organized in distinct concentric layers in contrast to the diarch to polyarch vascular tissues of the central stele. Up to now, the outermost layer of the stele, the pericycle, has always been regarded, in accordance with the outer tissue layers, as one uniform concentric layer. However, considering its lateral root-forming competence, the pericycle is composed of two different cell types, with one subset of cells being associated with the xylem, showing strong competence to initiate cell division, whereas another group of cells, associated with the phloem, appears to remain quiescent. Here, we established, using detailed microscopy and specific Arabidopsis thaliana reporter lines, the existence of two distinct pericycle cell types. Analysis of two enhancer trap reporter lines further suggests that the specification between these two subsets takes place early during development, in relation with the determination of the vascular tissues. A genetic screen resulted in the isolation of mutants perturbed in pericycle differentiation. Detailed phenotypical analyses of two of these mutants, combined with observations made in known vascular mutants, revealed an intimate correlation between vascular organization, pericycle fate, and lateral root initiation potency, and illustrated the independence of pericycle differentiation and lateral root initiation from protoxylem differentiation. Taken together, our data show that the pericycle is a heterogeneous cell layer with two groups of cells set up in the root meristem by the same genetic pathway controlling the diarch organization of the vasculature.
Involvement of Polyamine Oxidase in Wound Healing
Authors: Angelini Riccardo , Tisi Alessandra , Rea Giuseppina , Chen Martha M. , Botta Maurizio , Federico Rodolfo , Cona Alessandra 
Published: November 2007

AbstractHydrogen peroxide (H2O2) is involved in plant defense responses that follow mechanical damage, such as those that occur during herbivore or insect attacks, as well as pathogen attack. H2O2 accumulation is induced during wound healing processes as well as by treatment with the wound signal jasmonic acid. Plant polyamine oxidases (PAOs) are H2O2 producing enzymes supposedly involved in cell wall differentiation processes and defense responses. Maize (Zea mays) PAO (ZmPAO) is a developmentally regulated flavoprotein abundant in primary and secondary cell walls of several tissues. In this study, we investigated the effect of wounding on ZmPAO gene expression in the outer tissues of the maize mesocotyl and provide evidence that ZmPAO enzyme activity, protein, and mRNA levels increased in response to wounding as well as jasmonic acid treatment. Histochemically detected ZmPAO activity especially intensified in the epidermis and in the wound periderm, suggesting a tissue-specific involvement of ZmPAO in wound healing. The role played by ZmPAO-derived H2O2 production in peroxidase-mediated wall stiffening events was further investigated by exploiting the in vivo use of N-prenylagmatine (G3), a selective and powerful ZmPAO inhibitor, representing a reliable diagnostic tool in discriminating ZmPAO-mediated H2O2 production from that generated by peroxidase, oxalate oxidase, or by NADPH oxidase activity. Here, we demonstrate that G3 inhibits wound-induced H2O2 production and strongly reduces lignin and suberin polyphenolic domain deposition along the wound, while it is ineffective in inhibiting the deposition of suberin aliphatic domain. Moreover, ZmPAO ectopic expression in the cell wall of transgenic tobacco (Nicotiana tabacum) plants strongly enhanced lignosuberization along the wound periderm, providing evidence for a causal relationship between PAO and peroxidase-mediated events during wound healing.
The Catalytic Properties of Hybrid Rubisco Comprising Tobacco Small and Sunflower Large Subunits Mirror the Kinetically Equivalent Source Rubiscos and Can Support Tobacco Growth
Authors: Sharwood Robert Edward , von Caemmerer Susanne , Maliga Pal , Whitney Spencer Michael 
Published: November 2007

Abstract
Plastomic replacement of the tobacco (Nicotiana tabacum) Rubisco large subunit gene (rbcL) with that from sunflower (Helianthus annuus; rbcLS) produced tobaccoRst transformants that produced a hybrid Rubisco consisting of sunflower large and tobacco small subunits (LsSt). The tobaccoRst plants required CO2 (0.5% v/v) supplementation to grow autotrophically from seed despite the substrate saturated carboxylation rate, K m, for CO2 and CO2/O2 selectivity of the LsSt enzyme mirroring the kinetically equivalent tobacco and sunflower Rubiscos. Consequently, at the onset of exponential growth when the source strength and leaf LsSt content were sufficient, tobaccoRst plants grew to maturity without CO2 supplementation. When grown under a high pCO2, the tobaccoRst seedlings grew slower than tobacco and exhibited unique growth phenotypes: Juvenile plants formed clusters of 10 to 20 structurally simple oblanceolate leaves, developed multiple apical meristems, and the mature leaves displayed marginal curling and dimpling. Depending on developmental stage, the LsSt content in tobaccoRst leaves was 4- to 7-fold less than tobacco, and gas exchange coupled with chlorophyll fluorescence showed that at 2 mbar pCO2 and growth illumination CO2 assimilation in mature tobaccoRst leaves remained limited by Rubisco activity and its rate (approximately 11 μmol m−2 s−1) was half that of tobacco controls. 35S-methionine labeling showed the stability of assembled LsSt was similar to tobacco Rubisco and measurements of light transient CO2 assimilation rates showed LsSt was adequately regulated by tobacco Rubisco activase. We conclude limitations to tobaccoRst growth primarily stem from reduced rbcLS mRNA levels and the translation and/or assembly of sunflower large with the tobacco small subunits that restricted LsSt synthesis.
A Genomic and Expression Compendium of the ExpandedPEBPGene Family from Maize
Authors: Danilevskaya Olga N. , Meng Xin , Hou Zhenglin , Ananiev Evgueni V. , Simmons Carl R. 
Published: November 2007

AbstractThe phosphatidylethanolamine-binding proteins (PEBPs) represent an ancient protein family found across the biosphere. In animals they are known to act as kinase and serine protease inhibitors controlling cell growth and differentiation. In plants the most extensively studied PEBP genes, the Arabidopsis (Arabidopsis thaliana) FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) genes, function, respectively, as a promoter and a repressor of the floral transition. Twenty-five maize (Zea mays) genes that encode PEBP-like proteins, likely the entire gene family, were identified and named Zea mays CENTRORADIALIS (ZCN), after the first described plant PEBP gene from Antirrhinum. The maize family is expanded relative to eudicots (typically six to eight genes) and rice (Oryza sativa; 19 genes). Genomic structures, map locations, and syntenous relationships with rice were determined for 24 of the maize ZCN genes. Phylogenetic analysis assigned the maize ZCN proteins to three major subfamilies: TFL1-like (six members), MOTHER OF FT AND TFL1-like (three), and FT-like (15). Expression analysis demonstrated transcription for at least 21 ZCN genes, many with developmentally specific patterns and some having alternatively spliced transcripts. Expression patterns and protein structural analysis identified maize candidates likely having conserved gene function of TFL1. Expression patterns and interaction of the ZCN8 protein with the floral activator DLF1 in the yeast (Saccharomyces cerevisiae) two-hybrid assay strongly supports that ZCN8 plays an orthologous FT function in maize. The expression of other ZCN genes in roots, kernels, and flowers implies their involvement in diverse developmental processes.
Compound Leaf Development and Evolution in the Legumes
Authors: Champagne Connie E.M. , Goliber Thomas E. , Wojciechowski Martin F. , Mei Raymond W. , Townsley Brad T. , Wang Kan , Paz Margie M. , Geeta R. , Sinha Neelima R. 
Published: November 2007

AbstractAcross vascular plants, Class 1 KNOTTED1-like (KNOX1) genes appear to play a critical role in the development of compound leaves. An exception to this trend is found in the Fabaceae, where pea (Pisum sativum) uses UNIFOLIATA, an ortholog of the floral regulators FLORICAULA (FLO) and LEAFY (LFY), in place of KNOX1 genes to regulate compound leaf development. To assess the phylogenetic distribution of KNOX1-independent compound leaf development, a survey of KNOX1 protein expression across the Fabaceae was undertaken. The majority of compound-leafed Fabaceae have expression of KNOX1 proteins associated with developing compound leaves. However, in a large subclade of the Fabaceae, the inverted repeat–lacking clade (IRLC), of which pea is a member, KNOX1 expression is not associated with compound leaves. These data suggest that the FLO/LFY gene may function in place of KNOX1 genes in generating compound leaves throughout the IRLC. The contribution of FLO/LFY to leaf complexity in a member of the Fabaceae outside of the IRLC was examined by reducing expression of FLO/LFY orthologs in transgenic soybean (Glycine max). Transgenic plants with reduced FLO/LFY expression showed only slight reductions in leaflet number. Overexpression of a KNOX1 gene in alfalfa (Medicago sativa), a member of the IRLC, resulted in an increase in leaflet number. This implies that KNOX1 targets, which promote compound leaf development, are present in alfalfa and are still sensitive to KNOX1 regulation. These data suggest that KNOX1 genes and the FLO/LFY gene may have played partially overlapping roles in compound leaf development in ancestral Fabaceae but that the FLO/LFY gene took over this role in the IRLC.
Riboswitch Control of Gene Expression in Plants by Splicing and Alternative 3′ End Processing of mRNAs
Authors: Wachter Andreas , Tunc-Ozdemir Meral , Grove Beth C. , Green Pamela J. , Shintani David K. , Breaker Ronald R. 
Published: November 2007

Abstract
The most widespread riboswitch class, found in organisms from all three domains of life, is responsive to the vitamin B1 derivative thiamin pyrophosphate (TPP). We have established that a TPP-sensing riboswitch is present in the 3′ untranslated region (UTR) of the thiamin biosynthetic gene THIC of all plant species examined. The THIC TPP riboswitch controls the formation of transcripts with alternative 3′ UTR lengths, which affect mRNA accumulation and protein production. We demonstrate that riboswitch-mediated regulation of alternative 3′ end processing is critical for TPP-dependent feedback control of THIC expression. Our data reveal a mechanism whereby metabolite-dependent alteration of RNA folding controls splicing and alternative 3′ end processing of mRNAs. These findings highlight the importance of metabolite sensing by riboswitches in plants and further reveal the significance of alternative 3′ end processing as a mechanism of gene control in eukaryotes.
Saturated Very-Long-Chain Fatty Acids Promote Cotton Fiber and Arabidopsis Cell Elongation by Activating Ethylene Biosynthesis
Authors: Qin Yong-Mei , Hu Chun-Yang , Pang Yu , Kastaniotis Alexander J. , Hiltunen J. Kalervo , Zhu Yu-Xian 
Published: November 2007

Abstract
Fatty acids are essential for membrane biosynthesis in all organisms and serve as signaling molecules in many animals. Here, we found that saturated very-long-chain fatty acids (VLCFAs; C20:0 to C30:0) exogenously applied in ovule culture medium significantly promoted cotton (Gossypium hirsutum) fiber cell elongation, whereas acetochlor (2-chloro-N-[ethoxymethyl]-N-[2-ethyl-6-methyl-phenyl]-acetamide; ACE), which inhibits VLCFA biosynthesis, abolished fiber growth. This inhibition was overcome by lignoceric acid (C24:0). Elongating fibers contained significantly higher amounts of VLCFAs than those of wild-type or fuzzless-lintless mutant ovules. Ethylene nullified inhibition by ACE, whereas C24:0 was inactive in the presence of the ethylene biosynthesis inhibitor (l-[2-aminoethoxyvinyl]-glycine), indicating that VLCFAs may act upstream of ethylene. C24:0 induced a rapid and significant increase in ACO (for 1-aminocyclopropane-1-carboxylic acid oxidase) transcript levels that resulted in substantial ethylene production. C24:0 also promoted Ser palmitoyltransferase expression at a later stage, resulting in increased sphingolipid biosynthesis. Application of C24:0 not only stimulated Arabidopsis thaliana root cell growth but also complemented the cut1 phenotype. Transgenic expression of Gh KCS13/CER6, encoding the cotton 3-ketoacyl-CoA synthase, in the cut1 background produced similar results. Promotion of Arabidopsis stem elongation was accompanied by increased ACO transcript levels. Thus, VLCFAs may be involved in maximizing the extensibility of cotton fibers and multiple Arabidopsis cell types, possibly by activating ethylene biosynthesis.