bluuebunny/crossref_metadata_2025_split huggingface dataset:
PD-1–PD-1 ligand interaction contributes to immunosuppressive microenvironment of Hodgkin lymphoma
Authors: Yamamoto Ryo , Nishikori Momoko , Kitawaki Toshio , Sakai Tomomi , Hishizawa Masakatsu , Tashima Masaharu , Kondo Tadakazu , Ohmori Katsuyuki , Kurata Masayuki , Hayashi Takamasa , Uchiyama Takashi 
Published: January 2008

AbstractProgrammed death-1 (PD-1)–PD-1 ligand (PD-L) signaling system is involved in the functional impairment of T cells such as in chronic viral infection or tumor immune evasion. We examined PD-L expression in lymphoid cell lines and found that they were up-regulated on Hodgkin lymphoma (HL) and several T-cell lymphomas but not on B-cell lymphomas. PD-L expression was also demonstrated in primary Hodgkin/Reed-Sternberg (H/RS) cells. On the other hand, PD-1 was elevated markedly in tumor-infiltrating T cells of HL, and was high in the peripheral T cells of HL patients as well. Blockade of the PD-1 signaling pathway inhibited SHP-2 phosphorylation and restored the IFN-γ–producing function of HL-infiltrating T cells. According to these results, deficient cellular immunity observed in HL patients can be explained by “T-cell exhaustion,” which is led by the activation of PD-1–PD-L signaling pathway. Our finding provides a potentially effective immunologic strategy for the treatment of HL.
Erythroid defects in TRα−/− mice
Authors: Kendrick Tulene S. , Payne Christine J. , Epis Michael R. , Schneider Jessica R. , Leedman Peter J. , Klinken S. Peter , Ingley Evan 
Published: January 2008

Abstract
Thyroid hormone and its cognate receptor (TR) have been implicated in the production of red blood cells. Here, we show mice deficient for TRα have compromised fetal and adult erythropoiesis. Erythroid progenitor numbers were significantly reduced in TRα−/− fetal livers, and transit through the final stages of maturation was impeded. In addition, immortalized TRα−/− erythroblasts displayed increased apoptosis and reduced capacity for proliferation and differentiation. Adult TRα−/− mice had lower hematocrit levels, elevated glucocorticoid levels, and an altered stress erythropoiesis response to hemolytic anemia. Most TRα−/− animals contained markedly altered progenitor numbers in their spleens. Strikingly, 20% of TRα−/− mice failed to elicit a stress erythropoiesis response and recovered very poorly from hemolytic anemia. We conclude that an underlying erythroid defect exists in TRα−/− mice, demon-strating the importance of TRα to the erythroid compartment.
VAMP-8 segregates mast cell–preformed mediator exocytosis from cytokine trafficking pathways
Authors: Tiwari Neeraj , Wang Cheng-Chun , Brochetta Cristiana , Ke Gou , Vita Francesca , Qi Zeng , Rivera Juan , Soranzo Maria Rosa , Zabucchi Giuliano , Hong Wanjin , Blank Ulrich 
Published: January 2008

AbstractInflammatory responses by mast cells are characterized by massive exocytosis of prestored granular mediators followed by cytokine/chemokine release. The vesicular trafficking mechanisms involved remain poorly understood. Vesicular-associated membrane protein-8 (VAMP-8), a member of the soluble N-ethylmaleimide–sensitive factor (NSF) attachment protein receptor (SNARE) family of fusion proteins initially characterized in endosomal and endosomal-lysosomal fusion, may also function in regulated exocytosis. Here we show that in bone marrow–derived mast cells (BMMCs) VAMP-8 partially colocalized with secretory granules and redistributed upon stimulation. This was associated with increased SNARE complex formation with the target t-SNAREs, SNAP-23 and syntaxin-4. VAMP-8–deficient BMMCs exhibited a markedly reduced degranulation response after IgE+ antigen-, thapsigargin-, or ionomycin-induced stimulation. VAMP-8–deficient mice also showed reduced plasma histamine levels in passive systemic anaphylaxis experiments, while cytokine/chemokine release was not affected. Unprocessed TNF accumulated at the plasma membrane where it colocalized with a VAMP-3–positive vesicular compartment but not with VAMP-8. The findings demonstrate that VAMP-8 segregates secretory lysosomal granule exocytosis in mast cells from cytokine/chemokine molecular trafficking pathways.
Phosphatidylcholine-specific phospholipase C activation is required for CCR5-dependent, NF-kB–driven CCL2 secretion elicited in response to HIV-1 gp120 in human primary macrophages
Authors: Fantuzzi Laura , Spadaro Francesca , Purificato Cristina , Cecchetti Serena , Podo Franca , Belardelli Filippo , Gessani Sandra , Ramoni Carlo 
Published: January 2008

AbstractCCL2 (MCP-1) has been shown to enhance HIV-1 replication. The expression of this chemokine by macrophages is up-modulated as a consequence of viral infection or gp120 exposure. In this study, we show for the first time that the phosphatidylcholine-specific phospholipase C (PC-PLC) is required for the production of CCL2 triggered by gp120 in human monocyte-derived macrophages (MDMs). Using a combination of pharmacologic inhibition, confocal laser-scanner microscopy, and enzymatic activity assay, we demonstrate that R5 gp120 interaction with CCR5 activates PC-PLC, as assessed by a time-dependent modification of its subcellular distribution and a concentration-dependent increase of its enzymatic activity. Furthermore, PC-PLC is required for NF-kB–mediated CCL2 production triggered by R5 gp120. Notably, PC-PLC activation through CCR5 is specifically induced by gp120, since triggering CCR5 through its natural ligand CCL4 (MIP-1β) does not affect PC-PLC cellular distribution and enzymatic activity, as well as CCL2 secretion, thus suggesting that different signaling pathways can be activated through CCR5 interaction with HIV-1 or chemokine ligands. The identification of PC-PLC as a critical mediator of well-defined gp120-mediated effects in MDMs unravels a novel mechanism involved in bystander activation and may contribute to define potential therapeutic targets to block Env-triggered pathologic responses.
Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow
Authors: Okorie Uzoma M. , Denney William S. , Chatterjee Manash S. , Neeves Keith B. , Diamond Scott L. 
Published: January 2008

AbstractProtein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIbα and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/μm2 at wall shear rates of 100, 500, and 1000 s−1, respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood (± 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow.
Cognate CD4+ T-cell–dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes
Authors: Nobile Cinzia , Lind Marianne , Miro Francesc , Chemin Karine , Tourret Marie , Occhipinti Giovanni , Dogniaux Stéphanie , Amigorena Sebastian , Hivroz Claire 
Published: January 2008

AbstractDendritic cells (DCs) control T cell–based immunity. To do so they need to mature and migrate to sites of T-cell priming. We have previously shown that cognate interactions of human CD4+ T cells with DCs induce DC maturation. We show here that CC chemokines produced during antigen-specific T-DC interactions also induce strong morphologic modifications and migration of immature DCs. These modifications are required for efficient T-cell activation. Moreover, we show that CC chemokines produced during antigen-specific DC–T-cell interactions induce the dissolution of structures involved in cell motility and present on immature DCs (ie, podosomes). We thus propose a model in which chemokines secreted during Ag-specific contact between T cells and DCs induce disassembly of interacting and neighboring immature DC podosomes, leading to recruitment of more immature DCs toward sites of antigenic stimulation and to amplification of T-cell responses.
Spontaneous retropharyngeal haematoma: a case report
Authors: Singh Arvind , Ofo Enyi , Cumberworth Vincent 
Published: January 2008

Abstract

Introduction
Spontaneous retropharyngeal haematoma is an unusual condition. It has multiple aetiological factors and can present to a number of specialists including the otolaryngologist.

Case presentation
We describe a case of spontaneous retropharyngeal haematoma which demonstrates the dramatic presentation and emphasises the need for a conservative approach.

Conclusion
It is important to be aware of this unusual condition with its distinct presentation. Surgical intervention should be resisted unless a treatable aetiological factor is found or airway compromise occurs. Most cases will resolve with conservative management.
Diagnostic utility of the soluble triggering receptor expressed on myeloid cells-1 in bronchoalveolar lavage fluid from patients with bilateral lung infiltrates
Authors: Huh Jin Won , Lim Chae-Man , Koh Younsuck , Oh Yeon Mok , Shim Tae Sun , Lee Sang Do , Kim Woo Sung , Kim Dong Soon , Kim Won Dong , Hong Sang-Bum 
Published: January 2008

Abstract
Background
Differential diagnosis of patients with bilateral lung infiltrates remains a difficult problem for intensive care clinicians. Here we evaluate the diagnostic role of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in bronchoalveolar lavage (BAL) specimens from patients with bilateral lung infiltrates.

Methods
We conducted a prospective observational study on 80 patients with bilateral lung infiltrates with clinical suspicion of infectious pneumonia. Patients were categorized into three groups: bacterial or fungal infection, intracellular or viral infection, and noninfectious inflammatory disease. sTREM-1 concentrations were measured, and BAL fluid and Clinical Pulmonary Infection Score (CPIS) were analyzed.

Results
The sTREM-1 concentration was significantly increased in patients with bacterial or fungal pneumonia (n = 29, 521.2 ± 94.7 pg/ml), compared with that in patients with viral pneumonia, atypical pneumonia or tuberculosis (n = 14, 92.9 ± 20.0 pg/ml) or noninfectious inflammatory disease (n = 37, 92.8 ± 10.7 pg/ml). The concentration of sTREM-1 in BAL fluid, but not CPIS, was an independent predictor of bacterial or fungal pneumonia, and a cutoff value of more than 184 pg/ml yielded a diagnostic sensitivity of 86% and a specificity of 90%.

Conclusion
The sTREM-1 level in BAL fluid from patients with bilateral lung infiltrates is a potential marker for the differential diagnosis of pneumonia due to extracellular bacteria.
CPSARST: an efficient circular permutation search tool applied to the detection of novel protein structural relationships
Authors: Lo Wei-Cheng , Lyu Ping-Chiang 
Published: January 2008

AbstractCircular permutation of a protein can be visualized as if the original amino- and carboxyl termini were linked and new ones created elsewhere. It has been well-documented that circular permutants usually retain native structures and biological functions. Here we report CPSARST (Circular Permutation Search Aided by Ramachandran Sequential Transformation) to be an efficient database search tool. In this post-genomics era, when the amount of protein structural data is increasing exponentially, it provides a new way to rapidly detect novel relationships among proteins.
Natural and recombinant human glycodelin activate a proapoptotic gene cascade in monocyte cells
Authors: Tee Meng Kian , Vigne Jean-Louis , Yu Jie , Taylor Robert N 
Published: January 2008

AbstractGlycodelin-A (GdA) is a member of the superfamily of lipocalins and the predominant glycoprotein secreted by human and primate endometrium in the secretory and early pregnancy phases. GdA can inhibit NK cell activity, T cell proliferation, and chemotaxis of monocytes. Its physiological function is thought to mediate immunotolerance at the fetomaternal interface. In the present studies, we engineered recombinant Gd (rGd) in yeast and tested its biological effects on monocyte viability. rGd, like the natural, purified endometrial GdA, is glycosylated and secreted, and they both induced apototic changes in monocytic U937 cells and primary human monocytes. Trypan blue exclusion, nucleosome release, DNA laddering, and immunocytochemistry to detect free 3′-OH DNA ends were used to characterize the effects of GdA and rGd. Using U937 cells as a model, cDNA microarray analyses revealed several pro- and antiapoptotic genes that were up- and down-regulated, respectively, in accordance with the kinetics of rGd-induced monocyte cell death. Real-time RT-PCR confirmed that Bad, Bax, and TNF-R1 gene expression were increased, whereas Bcl-2A1 and a proliferation-inducing ligand (APRIL) were reduced by rGd. Transfection assays in U937 cells indicated that the immunomodulatory actions of rGd were associated with NF-κB inhibition. Western blotting of U937 and primary monocyte lysates demonstrated that rGd activated caspase-8, -2, and -3 to execute programmed cell death in these cells. We postulate that infiltrating monocytes and potentially other innate immune cells of the decidua might be manipulated by this glycoprotein to enhance embryonic implantation rates or conversely, to develop novel contraceptive strategies.
Platelet factor 4/CXCL4-stimulated human monocytes induce apoptosis in endothelial cells by the release of oxygen radicals
Authors: Woller Geske , Brandt Ernst , Mittelstädt Jessica , Rybakowski Christian , Petersen Frank 
Published: January 2008

AbstractThe generation of reactive oxygen species (ROS) represents a pivotal element of phagocyte defense against microbial invaders. However, oxidative stress also participates in pathophysiological processes of vascular damage leading to cell death of endothelial cells (EC). Currently, ROS-producing cells involved in this process as well as the corresponding extracellular signals required for their activation are ill-defined. In this study, we investigate the impact of the platelet-derived CXC chemokine platelet factor 4 (PF4/CXCL4) on the interaction of human monocytes and EC. We can show for the first time that PF4-activated monocytes become cytotoxic for EC but not epithelial cells. Cytotoxicity was time- and dose-dependent, and earliest effects were seen after 15 h of culture and at a concentration from 0.125 μM PF4 up. By performing transwell experiments and by using specific inhibitory antibodies, we could show that direct cell contact between effector and target cells, mediated by β2integrins as well as their corresponding ligand ICAM-1, is essential for the cytotoxic effect. Investigations of the cellular mechanisms of cytotoxicity revealed that in the presence of EC, PF4-activated monocytes are capable of releasing high amounts of ROS for more than 2 h following stimulation. This causes programmed cell death in EC, as inhibitors of the NADPH oxidase (diphenyleneiodonium and apocynin) effectively blocked PF4-induced monocyte oxidative burst and protected EC from undergoing apoptosis. Taken together, our data suggest a role for platelet-derived PF4 in oxidative stress-mediated vascular disorders, as observed during atherosclerosis or ischemia/reperfusion injury.
Ein unbekanntes Ehrenmonument des Kaisers Phokas aus Synada bei Akronion (Afyon)
Authors: Asutay Neslihan 
Published: January 2008

Abstract
Im Garten des Museums von Afyon, dem antiken Akronion, befinden sich zwei Teile eines monumentalen Kapitells. Seine beträchtlichen Maße und das Kaisermonogramm (s.u.) lassen in ihm den Rest eines Ehrenmonuments erkennen (Höhe ca. 1.00 m, obere Breite ca. 1.00 m). Nach Auskunft der Museumsleitung wurde es Mitte der 80er Jahre in Huhut (das antike Synada, 26 km südlich von Akronion/Afyon) bereits beschädigt entdeckt und war während des Transports ins Museum in zwei Teilen zerbrochen. Weitere Schäden enstanden an der Halspartie, an zwei Schauseiten sowie an der oberen Platte. Auf der Oberseite befinden sich keinerlei Einlassungen für eine Statue oder einen Kämpfer. Das Kapitell wurde aus „synadischem Marmor” gefertigt, der tatsächlich in den Marmorbrüchen des phrygischen Dokimaion (heutige Isçehisar, 21 km nordöstlich von Afyon) gewonnen wird. Der weiße Marmor von Dokimaion wird im heutigen türkischen Sprachgebrauch als „Afyon-Kaymak/Afyon-Sahne”, „Afyon-Beyaz/Afyon-Weiss” oder „Afyon-Şeker/Afyon-Zucker” bzw. als „Afyon-Çizgisiz-Şeker/Afyon-Zucker ohne Linien” bezeichnet.
G. DEMETROKALLES, Bυζαντινή ναοδομία στην Νάξο…
Authors: Altripp Michael 
Published: January 2008

Abstract
Der schmale, aber großformatige Band stellt den Neuabdruck eines Beitrages des Verfassers in der Beilage Eπtά ημέϱες der Tageszeitung Kαθημεϱινή vom 13.2.1997 dar. Vorangestellt ist ein Vorwort, dem sich nach einer kurzen Einführung die Kapitel über die frühchristliche und byzantinische Zeit anschließen. Die mittelalterlichen Kirchen gliedert der Autor nach typologischen Gesichtspunkten und unterscheidet die Bauten vom Typus des freistehenden Kreuzes, mit eingeschriebenem Kreuz, mit trikonchalem Naos sowie die einschiffigen Anlagen. Zuletzt folgt ein Kapitel über die architektonische Morphologie, worunter Mauerwerk, Bauformen und Bauausstattung zusammengefaßt werden. Eine Bibliographie rundet den Band ab.
L. DOĞER, Izmir Arkeoloji Müzesi Örnekleriyle Kazιma Dekorlu Ege-Bizans Seramikleri
Authors: Böhlendorf-Arslan Beate 
Published: January 2008

Abstract
Im Archäologischen Museum in Izmir sind 227 Gefäße inventarisiert, die einen der größten Bestände von Unterwasserfunden byzantinischer Keramik in der Türkei bilden. Aus dieser Sammlung stellt Lale Doğer in dem vorliegenden Band, einem Ausschnitt ihrer Doktorarbeit, 123 dieser Vasen vor. Die Keramiken des Museums Izmir wurden zwischen 1967 und 1974 im Kunsthandel gekauft bzw. als illegales Gut beschlagnahmt. 166 Gefäßen wurden aus anderen türkischen Museen nach Izmir überwiesen (Vorwort S. 1-2) und stammen wohl ebenso aus ungeklärten Fundzusammenhängen. Eine genauere Fundortangabe besitzt daher keines der Stücke. Die Autorin verbindet die Exponate in ihrem Schlusswort (S. 74) mit einem unmittelbar vor der türkischen Küste gesunkenen Boot oder aber auch dem bekannten Schiffswrack bei der wenig vor der türkischen Küste gelegenen griechischen Insel Kastellorizo (neu auch bei: D. Papanikola-Bakirtzi (Hrsg.), Byzantine Glazed Ceramics. The Art of Sgraffito, Athen 1999). Die größte Gruppe bildet die von Megaw definierte Ägäische Ware (A. H. S. Megaw in: G. Robertson u. G. Henderson (Hrsg.), Studies in Memory of David Talbot Rice, Edinburgh 1975, 34-45) mit 114 Stücken. Andere Vasen gehören zur Engobebemalten Ware („Slip Painted Ware”) oder zu mit Farboxyden bemalten Warengruppen (S. 2). In der vorliegenden Abhandlung behandelt Doğer ausschließlich die Ägäische Ware.
J. KODER u.a., Das Register des Patriarchats von Konstantinopel, 3. Teil. Edition und Übersetzung der Urkunden aus den Jahren 1350–1363
Authors: Tinnefeld Franz 
Published: January 2008

Abstract
Sechs Jahre nach dem 2. Teil (vgl. die Rezensionen in BZ 90, 1997, 125f. von C. Mango und ByzSlav 57, 1996, 399–401 von F. T.) ist nun der 3. Teil des Patriarchatsregisters von Konstantinopel (PRK) erschienen. Er enthält die Urkunden Nr. 176–271, die mit wenigen Ausnahmen (s. u.) dem ersten Patriarchat des Kallistos, dem ersten Patriarchat des Philotheos und dem zweiten Patriarchat des Kallistos entstammen.
W. TREADGOLD, A History of the Byzantine State and Society
Authors: Brandes Wolfram 
Published: January 2008

Abstract
To review a book that deals with 1000 years of Byzantine history is just as bold an undertaking as the writing of the book itself. Treadgold (henceforth T.) took this challenge on, and we should be grateful to him for doing so. The reviewer admits that he is not informed equally well over all periods of the history of the Byzantine empire – there is probably no-one on the planet who could with a clear conscience assert that they were. In this respect the reviewer admires T. for his lack of reserve in expressing his views on problems and periods of Byzantine history with which he is clearly less familiar than with those of the eighth and ninth centuries.
J. WITT (Hrsg.), Staatliche Museen zu Berlin-Preußischer Kulturbesitz. Bestandskataloge, Band 2. Werke der Alltagskultur, Teil 1: Menasampullen.
Authors: Elbern Victor H. 
Published: January 2008

Abstract
Mit dem «Catalogue raisonné» von Oskar Wulff: „Altchristliche und mittelalterliche Bildwerke“ (1909 bzw. 1911) war die Erforschung und Präsentation der in den Staatlichen Museen zu Berlin gesammelten einschlägigen Kunst seinerzeit mit einem Schlage in die vorderste Reihe wissenschaftlicher Kataloge in Europa gerückt worden. Abgesehen von einer Neubearbeitung durch Wolfgang Fritz Volbach (1950) ist entsprechend Umfassendes für die ehemalige „Frühchristlich-Byzantinische Sammlung“ der Berliner Museen seither nicht wieder geleistet worden. Mit dem beträchtlichen zeitlichen Abstand zu den älteren Katalogen erscheint es verständlich und begrüßenswert, wenn – nach der Zusammenführung der seit 1945 zwischen Ost und West geteilten musealen Bestände – eine neue Bestandsaufnahme unternommen wird. Das anspruchsvolle, dem Geleitwort von Arne Effenberger zufolge auf 10 Bände (mit Unterteilungen) angelegte Vorhaben verspricht noch umfassender und auf seine Art vorbildlich zu werden, wie es die Kataloge von Wulff waren. Inzwischen ist ein erster Teil von Band 1 über die Textiliensammlung erschienen. Wie dieser ist auch der 2. Band über „Werke der Alltagskultur“ auf mehrere Teilbände angelegt, deren eben erschienener erster Part ausschließlich den sog. Menasampullen gewidmet ist. Es handelt sich dabei um die bekannten Pilgerandenken aus dem unweit Alexandria in der Mariotis gelegenen Wüstenheiligtum des hl. Menas. Es geht freilich nicht um die erste Bearbeitung dieser kleinformatigen, auch von Privatsammlern hochgeschätzten Objekte: es sei verwiesen auf ältere Arbeiten von C.M. Kaufmann, dem Ausgräber von Abu Mina, sowie aus jüngster Zeit auf die Veröffentlichungen von C. Metzger zu den Sammlungen des Louvre (1981) und G. Kaminski-Menssen zu den Beständen des Liebieg-Hauses in Frankfurt (1997). Berlin nimmt mit 63 Menas-Ampullen immerhin einen bedeutenden Platz ein.
Two-dimensional homo- and hetero-correlation technique applied to NIR and py-MBMS spectra of wood
Authors: Labbé Nicole , André Nicolas , Rials Timothy G. , Kelley Stephen S. 
Published: January 2008

Abstract
In this work, near infrared (NIR) and pyrolysis-molecular beam mass spectra (MBMS) of loblolly pine (Pinus taeda) were studied by means of generalized two-dimensional correlation spectroscopy in order to assign specific contributions of cellulose in the two spectral domains. Homo- and hetero-correlation techniques were employed to analyze the concentration-dependent spectral variations of cellulose. Specific bands of cellulose were assigned in the NIR and MBMS spectra, and moreover two masses m/z at 114 and 173, commonly assigned to cellulose fragments, were found to not originate from the pyrolysis of cellulose.