bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acceleration of hyperfractionated chemoradiation regimen for advanced head and neck cancer
Authors: Allen Aaron M. , Elshaikh Mohamed , Worden Francis P. , Bradford Carol R. , Teknos Theodoros N. , Chepeha Douglas B. , Tsien Christina , Dawson Laura A. , Urba Susan , Wolf Gregory T. , Normolle Daniel , Eisbruch Avraham 
Published: November 2006

AbstractBackground.Our aim was to evaluate the acceleration of a hyperfractionated, concurrent chemoradiation regimen (HxCRT) for advanced head and neck squamous cell carcinoma (HNSCC).Methods.Patients with unresectable HNSCC were treated based on a previously published HxCRT regimen: 1.25 Gy twice daily to 70 Gy concurrent with cisplatin 12 mg/m2/day and5‐fluorouracil 600 mg/m2/day for 5 days, weeks 1, 5. This regimen was accelerated in this series by shortening the treatment from 7 to 6 weeks by omitting the planned mid‐treatment 1‐week break.Results.Forty‐six patients with T3‐4/N3 disease were treated. The main acute toxicity was pharyngeal. Median weight change during therapy in patients with and without enteral feeding tubes was −3.8% and −7.9%, respectively (p = .08). Fifteen percent had late grade III pharyngeal toxicity. Local/regional and distant failure rates were 28% and 17%, respectively; 52% are alive without evidence of disease.Conclusions.In nonresectable HNSCC, acceleration of the HxCRT regimen is feasible, requiring enteral feeding tubes during therapy in most patients. © 2006 Wiley Periodicals, Inc. Head Neck, 2007
Influence of social support on health‐related quality of life outcomes in head and neck cancer
Authors: Karnell Lucy Hynds , Christensen Alan J. , Rosenthal Eben L. , Magnuson J. Scott , Funk Gerry F. 
Published: November 2006

AbstractBackground.Evidence that social support influences health‐related quality of life (HRQOL) in oncologic patients could be particularly important for head and neck cancer because this disease can affect speech, eating, and facial aesthetics.Methods.Multiple regression analyses were used in this prospective, observational study to determine the association between 394 patients' ratings of perceived post‐treatment social support and HRQOL outcomes while controlling for possible confounding variables.Results.Higher social support scores were significantly associated with higher scores in speech (p = .007), aesthetics (p = .015), social disruption (p = .045), and general mental health (p = .016) and with fewer depressive symptoms (p = .023) but not with general physical health (p = .191) or eating (p = .114). The magnitude of differences in the HRQOL outcomes for patients whose social support scores fell in the lowest and highest quartiles were clinically meaningful.Conclusions.Given the association between social support and HRQOL outcomes in this patient population, modification of perceived social support through clinical interventions could improve the survivorship of these patients. © 2006 Wiley Periodicals, Inc. Head Neck, 2007
Use of integrated 18F‐FDG PET/CT to improve the accuracy of initial cervical nodal evaluation in patients with head and neck squamous cell carcinoma
Authors: Jeong Han‐Sin , Baek Chung‐Hwan , Son Young‐Ik , Ki Chung Man , Kyung Lee Dong , Young Choi Joon , Kim Byung‐Tae , Kim Hyung‐Jin 
Published: November 2006

AbstractBackground.We investigated the accuracy of performing cervical nodal evaluation with using integrated 18F‐fluoro deoxyglucose positron emission tomography (PET)/CT for squamous cell carcinoma (SCC) of the head and neck as compared with using PET and contrast‐enhanced CT (CECT) alone.Methods.The presence of metastatic lymphadenopathy in each cervical nodal group (level I–VI) and the nodal (N) classification of 47 patients with SCC of the head and neck were determined by using PET, CECT, and PET/CT, respectively, and the results were verified according to the histopathologic findings.Results.Among the 91 foci that had abnormal uptake on PET, the combined PET/CT images provided additional information over PET for the anatomical localization and lesion characterization of 18 sites (19.8%) in 17 patients (36.2%). PET/CT also showed the best results among the three imaging modalities for the sensitivity, specificity, and accuracy (91.8, 98.9, and 97.1%, respectively) for predicting metastatic nodes on a level‐by‐level analysis, and PET/CT had a higher accuracy (85.1%) for the pathologic nodal classification over the clinical examinations (68.1%) or PET (70.2%).Conclusions.Combined PET/CT images are more accurate than the PET or CECT images alone for conducting cervical node evaluation in the patients suffering with head and neck SCC. © 2006 Wiley Periodicals, Inc. Head Neck, 2007
Successful implant of long‐term cryopreserved parathyroid glands after total parathyroidectomy
Authors: de Menezes Montenegro Fábio Luiz , Custódio Melani Ribeiro , Arap Sérgio Samir , Reis Luciene Machado dos , Sonohara Shigueko , Castro Inês Vieira , Jorgetti Vanda , Cordeiro Anói Castro , Ferraz Alberto Rosseti 
Published: November 2006

AbstractBackground.Parathyroid cryopreservation is essential in some cases of parathyroid surgery. The fate of autografted tissue after long‐term cryopreservation is not fully discussed in the literature.Methods.The successful experience with the use of parathyroid tissues preserved for 21 months and 30 months is reported.Results.Both patients were women with renal hyperparathyroidism who underwent total parathyroidectomy without autotransplantation. Patient 1 was a 40‐year‐old woman. At 21 months of follow‐up, her parathyroid hormone (PTH) level was undetectable, and despite oral calcium supplements, she was hypocalcemic. Forty‐five cryopreserved fragments were thawed and implanted in her forearm. Calcium levels improved, and PTH steadily increased in both arms. PTH levels at 18 months after the autograft were 37.0 pg/mL in the contralateral arm and 1150.0 pg/mL in the implant arm. Patient 2 was a 44‐year‐old woman. After 30 months, her PTH was undetectable, and she underwent cryopreserved tissue implantation.Conclusion.These cases show that parathyroid tissue may remain viable even after long‐term storage. © 2006 Wiley Periodicals, Inc. Head Neck, 2007
Aberrant cytokine expression in serum of patients with adenoid cystic carcinoma and squamous cell carcinoma of the head and neck
Authors: Hoffmann Thomas K. , Sonkoly Eniko , Homey Bernhard , Scheckenbach Katrin , Gwosdz Christian , Bas Murat , Chaker Adam , Schirlau Kerstin , Whiteside Theresa L. 
Published: November 2006

AbstractBackground.Squamous cell carcinoma (SCC) and adenoid cystic carcinoma (ACC) represent 2 clinically important subtypes of head and neck cancer. Our objective was to characterize and compare cytokine profiles in the systemic circulation of patients with SCC and ACC.Methods.Multiplex analysis of 10 different cytokines (interleukin [IL]‐1β, IL‐2, IL‐4, IL‐5, IL‐6, IL‐8, IL‐10, granulocyte‐macrophage colony‐stimulating factor [GM‐CSF], interferon [IFN]‐γ, and tumor necrosis factor [TNF]‐α) in the serum of patients with SCC (n = 20) and ACC (n = 20) and healthy controls (n = 20) was performed using the Luminex fluorescent‐bead technology.Results.Patients with SCC as well as patients with ACC showed an altered cytokine profile compared with healthy individuals. In patients with SCC, significantly elevated serum levels of the proinflammatory cytokines, IL‐6 and IL‐8, were observed. In patients with ACC, IL‐8 serum levels were significantly elevated, and IL‐6 serum levels were only increased in a subset of patients.Conclusions.A similar serum cytokine profile, with the predominance of proinflammatory cytokines, was observed in patients with SCC and ACC. The newly defined cytokine profile in ACC patients may form the basis for future investigations to explore the role of cytokines in ACC tumor progression and their potential value as predictive biomarkers. © 2006 Wiley Periodicals, Inc. Head Neck, 2007
Impaired maze performance in aged rats is accompanied by increased density of NMDA, 5‐HT1A, and α‐adrenoceptor binding in hippocampus
Authors: Topic B. , Willuhn I. , Palomero‐Gallagher N. , Zilles K. , Huston J.P. , Hasenöhrl R.U. 
Published: November 2006

AbstractUsing quantitative receptor autoradiography, we assessed binding site densities and distribution patterns of glutamate, GABAA, acetylcholine (ACh), and monoamine receptors in the hippocampus of 32‐month‐old Fischer 344/Brown Norway rats. Prior to autoradiography, the rats were divided into two groups according to their retention performance in a water maze reference memory task, which was assessed 1 week after 8 days of daily maze training. The animals of the inferior group showed less long‐term retention of the hidden‐platform task but did not differ from superior rats in their navigation performance during place training and cued trials. The decreased retention performance in the group of inferior learners was primarily accompanied by increased α1‐adrenoceptors in all hippocampal subregions under inspection (CA1‐CA4 and dentate gyrus), while elevated α2‐adrenoceptor binding was observed in the CA1 region and DG. Furthermore, inferior learners had higher NMDA binding in the CA2 and CA4 and increased 5‐HT1A binding sites in the CA2, CA3, and CA4 region. No significant differences between inferior and superior learners were evident with regard to AMPA, kainate, GABAA, muscarinergic M1, dopamine D1, and 5‐HT2 binding densities in any hippocampal region analyzed. These results show that increased NMDA, 5‐HT1A, and α‐adrenoceptor binding in the hippocampus is associated with a decline in spatial memory. The increased receptor binding observed in the group of old rats with inferior maze performance might be the result of neural adaptation triggered by age‐related changes in synaptic connectivity and/or synaptic activity. © 2006 Wiley‐Liss, Inc.
Risk factors for intrahepatic cholangiocarcinoma in a low‐risk population: A nationwide case‐control study
Authors: Welzel Tania M. , Mellemkjaer Lene , Gloria Gridley , Sakoda Lori C. , Hsing Ann W. , Ghormli Laure El , Olsen Jorgen H. , McGlynn Katherine A. 
Published: November 2006

AbstractRecently, the incidence of intrahepatic cholangiocarcinoma (ICC) has been increasing in a number of developed (Western) countries. However, risk factors in these low‐risk populations are poorly understood. In this nationwide population based case‐control study in Denmark, we examined the relationship between selected medical conditions and subsequent ICC risk to provide additional clues to etiopathogenesis. All histologically confirmed ICC cases diagnosed in Denmark between 1978 and 1991 were identified from the Danish cancer registry. Population controls were selected from the central population registry and were matched 4:1 to cases on sex and year of birth. Cases and controls were linked to the Danish hospital discharge registry to obtain information on prior hospital diagnoses. Odds ratios (OR) and 95% confidence intervals (95% CI) were derived using conditional logistic regression. A total of 764 ICC cases and 3,056 population controls were included in the study. Chronic liver diseases were significantly related to ICC: alcoholic liver disease (OR = 19.22, 95% CI = 5.55–66.54), unspecified cirrhosis (OR = 75.9, 95% CI 10.2–565.7). Bile duct diseases were also associated with risk: cholangitis (OR = 6.3, 95% CI = 2.3–17.5), choledocholithiasis (OR = 23.97, 95% CI = 2.9–198.9), cholecystolithiasis (OR = 4.0, 95% CI = 2.0–7.99), though gallbladder removal did not change risk (OR = 1.6, 95% CI = 0.65–3.7). Among other conditions, chronic inflammatory bowel disease (OR = 4.7, 95% CI = 1.65–13.9) was significantly associated with ICC. Diabetes was associated with risk in the year prior to diagnosis of ICC (OR = 3.02, 95% CI = 1.05–8.69). Obesity was unrelated to risk. These results confirm that prior bile duct diseases increase risk of ICC and suggest that alcoholic liver disease and diabetes may also increase risk. © 2006 Wiley‐Liss, Inc.
A novel ordered nano hydroxyapatite coating electrochemically deposited on titanium substrate
Authors: Hu Ren , Lin Chang‐Jian , Shi Hai‐Yan 
Published: November 2006

AbstractA novel porous nano hydroxyapatite (HA) coating has been prepared on commercially pure titanium substrate by a modified electrochemical deposition method. The physico‐chemical and biological properties of the coating were characterized by SEM, XRD, FTIR, Raman, and in vitro cell culture test respectively. The SEM patterns show a uniform microporous morphology consisting of wirelike crystals at nanometer scale. It is suggested that under controlled deposition conditions, the primary HA nanowires grow and self‐assemble to construct an ordered microporous nest‐like morphology, thus to form a nano‐micro two‐level structure. The XRD results demonstrate that the HA nanowires are orderly arranged with their c‐axis preferentially perpendicular to the substrate surface. The Raman and IR spectra affirm that the main component of the coating is well crystallized HA. An interdigitation phenomenon of the MG63 human osteosarcoma cells with the HA nanowires is observed in the in vitro test, indicating excellent biocompatibility and bioactivity for the prepared coating. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Plasma deposition of tetraglyme inside small diameter tubing: Optimization and characterization
Authors: Cao Lan , Ratner Buddy D. , Horbett Thomas A. 
Published: November 2006

AbstractIn this study, a glow discharge plasma deposition system previously used for treating flat substrates was successfully modified and optimized to produce a PEO‐like coating on the inner surface of 1–3 mm ID polyethylene tubing by deposition of tetra ethylene glycol dimethyl ether (tetraglyme). The plasma treatment conditions were varied in order to find operating values that would produce coatings with the ultralow (<5 ng/cm2) fibrinogen adsorption (ΓFg) previously shown necessary to significantly reduce platelet adhesion. The flow rate of gaseous tetraglyme monomer, pressure, and plasma generating power were found to be the most important parameters affecting the uniformity and chemical structure of the coating. The coating uniformity and quality were assessed by measuring ΓFg at positions 1 cm apart along the entire tube and the fraction of C1s carbon that was in an ether bond (ether–carbon ratio) by electron spectroscopy of chemical analysis. Under optimized conditions, tetraglyme plasma‐coated tubes of up to 20 cm in length had ultralow ΓFg. The region of the tube that had ultralow ΓFg also had C1s ether–carbon ratios that are greater than 50%. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Investigation ofin vitrobiocompatibility of novel pentablock copolymers for gene delivery
Authors: Agarwal Ankit , Unfer Robert , Mallapragada Surya K. 
Published: November 2006

AbstractNovel pentablock copolymers of poly(diethylaminoethylmethacrylate) (PDEAEM), poly(ethylene oxide) (PEO), and poly(propylene oxide) (PPO), (PDEAEM‐b‐PEO‐b‐PPO‐b‐PEO‐b‐PDEAEM), were synthesized as vectors for gene delivery, and were tested for their biocompatibility on SKOV3 (human ovarian carcinoma) and A431 (human epidermoid cancer) cell lines under differentin vitroconditions using various assays to elucidate the mechanism of cell death. These copolymers form micelles in aqueous solutions and can be tuned for their cytotoxicity by tailoring the weight percentage of their cationic component, PDEAEM. Copolymers with higher PDEAEM content were found to be more cytotoxic, though their polyplexes were less toxic than the polycations alone. Pentablock copolymers displayed higher cell viability than commercially available ExGen 500® at similar N:P ratios. While cell death with ExGen was found to be accompanied by an early loss of cell membrane integrity, pentablock copolymers caused very little membrane leakage. Caspase‐3/7 assay confirmed that none of these polymers induced apoptosis in the cells. These pentablock copolymers form thermo‐reversible gels at physiological temperatures, thereby enabling controlled gene delivery. Toxicity of the polymer gels was tested using an agarose‐matrix, simulating anin vivotumor model where injected polyplex gels would dissolve to release polyplexes, diffusing through tumor mass to reach the target cells. Twenty five weight percent of copolymer gels were found to be nontoxic or mildly cytotoxic after 24 h incubation. Transfection efficiency of the copolymers was found to be critically correlated to cytotoxicity and depended on DNA dose, polymer concentration, and N:P ratios. Transgene expression obtained was comparable to that of ExGen, but ExGen exhibited greater cell death. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Three‐dimensional growth behavior of osteoblasts on biomimetic hydroxylapatite scaffolds
Authors: Rumpler M. , Woesz A. , Varga F. , Manjubala I. , Klaushofer K. , Fratzl P. 
Published: November 2006

AbstractThe authors used rapid prototyping to produce three‐dimensional hydroxylapatite scaffolds with controlled, fully interconnected porosity. The purpose of this study was to illuminate the effect of hormones on the osteogenic differentiation and to investigate how osteoblasts colonize the three‐dimensional scaffold focusing on the formation of the cellular network. Preosteoblasts were seeded onto scaffolds, were optionally treated with the osteogenic hormones triiodo‐L‐thyronine (T3) and 1,25‐dihydroxyvitamin‐D3 (D3), and the expression of osteoblastic marker genes was investigated. Confocal laser scanning microscopy was used to investigate the three‐dimensional growth behavior. Culturing cells on scaffolds strongly increased the expression of osteocalcin, osteoprotegerin, Runx2, and receptor activator of NFkB‐ligand (RANKL). Treatment with T3 increased the expression of osteocalcin but did not change that of osteoprotegerin and Runx2. Treatment with D3 inhibited the expression of osteocalcin, Runx2, and osteoprotegerin. Both hormones had similar effects in the three‐dimensional system as found in two‐dimensional cultures although more accentuated, indicating that preosteoblasts behave more naturally on three‐dimensional structures. The osteoblasts colonized the three‐dimensional squared pores of scaffolds by forming a cellular network with a round central channel keeping it into the depth and depositing collagen fibrils. These results provide insight how osteoblasts colonize a three‐dimensional system and underline the importance of this environment in osteoblastic differentiation studies. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Direct encapsulation of BSA and DNA into silica microcapsules (hollow spheres)
Authors: Fujiwara Masahiro , Shiokawa Kumi , Hayashi Kaoru , Morigaki Kenichi , Nakahara Yoshiko 
Published: November 2006

AbstractWe reported before that silica hollow spheres (microcapsules) are prepared by interfacial reaction method that W/O emulsion with the aqueous solution of sodium silicate and n‐hexane solution of Tween 80 and Span 80 is combined with another aqueous solution of silica precipitant such as NH4HCO3 and NH4Cl. This procedure using W/O/W emulsion fabricates the hollow structures of silica particles directly, and additional steps such as the removal of core parts, that are often essential for the preparation of hollow particles via core‐shell materials, are not required. When biomacromolecules such as protein and nucleic acid are mixed in the aqueous solution of sodium silicate, these macromolecules can be encapsulated into the microcapsules. We succeeded the direct encapsulation of bovine serum albumin (BSA) and duplex DNA. Most of encapsulated BSA and DNA cannot be released from the microcapsules without the destruction of microcapsule shell. These microcapsule materials encapsulating biomacromolecules will be applied to biotechnologies such as immobilized enzyme and so on. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Adhesion of MC3T3‐E1 cells to RGD peptides of different flanking residues: Detachment strength and correlation with long‐term cellular function
Authors: Lee Mark H. , Adams Christopher S. , Boettiger David , DeGrado William F. , Shapiro Irving M. , Composto Russell J. , Ducheyne Paul 
Published: November 2006

AbstractWe synthesized a series of RGD peptides and immobilized them to an amine‐functional self‐assembled monolayer using a modified maleimide‐based conjugate technique that minimizes nonspecific interactions. Using a spinning disc apparatus, a trend in the detachment strength (τ50) of RGD peptides of different flanking residues was found: RGDSPK > RGDSVVYGLR ≈ RGDS > RGES. Using blocking monoclonal antibodies, cellular adhesion to the peptides was shown to be primarily αv‐integrin‐mediated. In contrast, the τ50 value of the cells on fibronectin (Fn)‐coated substrates of similar surface density was 6–7 times higher and involved both α5β1 and αvβ3 integrins. Cellular spreading was enhanced on RGD peptides after 1 h when compared to RGE and unmodified substrates. However, no significant differences were observed between the different RGD peptides. Long‐term function of MC3T3‐E1 cells was also evaluated by measuring alkaline phosphatase (ALP) activity and mineral deposition. Among the four peptides, RGDSPK exhibited the highest level of ALP activity after 11 days and mineralization after 15 days and reached comparable levels as Fn substrates after 15 and 24 days, respectively. These findings collectively illustrate both the advantages and limitations of enhancing cellular adhesion and function by the design of RGD peptides. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Microstructure and macroscopic properties of bioactive CaO–SiO2–PDMS hybrids
Authors: Salinas A. J. , Merino J. M. , Babonneau F. , Gil F. J. , Vallet‐Regí M. 
Published: November 2006

AbstractCaO–SiO2–PDMS (polydimethylsiloxane) hybrid materials were synthesized as crack‐free monoliths presenting in vitro bioactivity, i.e. able to be coated with a calcium phosphate‐rich layer after having been soaked in simulated body fluid (SBF). A wide physical–chemical characterization of these materials was carried out to relate their microscopic structure and macroscopic properties. The effect of PDMS and the amounts of water used for the tetraethoxysilane (TEOS) hydrolysis on the mechanical properties of hybrid materials was investigated by three‐point bending tests. For a given amount of water, as PDMS content in hybrids increased, the elastic modulus decreased. Furthermore, keeping the PDMS content constant, when the amount of H2O decreased, the elastic modulus increased. Regarding in vitro bioactivity and mechanical properties, the hybrid material obtained with molar ratios H2O/TEOS = 2 and TEOS/PDMS = 3.5 proved to be the best candidate for either soft tissue substitution or metallic implant coating since the hybrid material would promote bond to bone formation, simultaneously dampening the mechanical charges. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006