bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prey availability and diet of the Eurasian otter (Lutra lutra) on a large reservoir and associated tributaries
Authors: Sales-Luís T. , Pedroso N.M. , Santos-Reis M. 
Published: January 2008

Eurasian otter, Lutra lutra (L., 1758), spraints collected at the Aguieira hydroelectric dam (central Portugal, n = 916) and tributaries (n = 412) were analysed to assess diet compared with prey availability. Fish and crayfish abundances in the reservoir were assessed with fyke and trammel nets. Prey availability in the tributaries was estimated by electrofishing. In both the reservoir and its tributaries, fish were the main prey and consisted predominantly of Lepomis gibbosus (L., 1758) (60%–65% of occurrences), which is an introduced species of Centrachidae that is abundant in the reservoir but is almost absent in the tributaries. These data suggest that otters using the tributaries feed predominantly in the reservoir. Seasonal dietary variations corresponded to increased availability of nonfish prey categories. Capture vs. consumption analyses demonstrated that otters did not consume L. gibbosus according to its availability. In fact, L. gibbosus was consumed less frequently when other prey species were more available (e.g., amphibians, eels, barbells). The tributaries offer important otter refuge areas that are scarce at the edge of the reservoir, and so, in large reservoirs care should be taken to minimize disturbance in the surrounding catchments. Results indicate that otter populations make use of these conjoint systems to ensure their survival.
At what distance do wolves move away from an approaching human?
Authors: Karlsson J. , Eriksson M. , Liberg O. 
Published: January 2008

Management of controversial animals, like large carnivores, in multi-use landscapes is highly dependent on human tolerance. Human fear of wolves ( Canis lupus L., 1758) is known to be an important variable affecting human attitudes towards wolves. Successful management of wolves thus forces managers to respond to human fear of wolves. In this study, we assess the distance at which radio-collared wolves move away from an approaching human, also called the flight initiation distance (FID), and how FID is affected by wind speed and wind direction. We used silent VHF receivers and walked at normal pace towards radio-collared wolves. When approaching the wolves, we made no attempt to hide or walk in a less noisy manner. In the 34 experimental provocations, wolves moved away when the approaching human was between 17 and 310 m away. The mean FID was 106 m. FID was negatively correlated with wind speed. One hour after having been roused by the approaching human, wolves had moved a median distance of 1.2 km.
N.F.S. Grundtvig, Niels Bukh and Other 'Japanese' Heroes. The Educators Obara Kuniyoshi and Matsumae Shigeyoshi and Their Lessons from the Past of a Foreign Country
Authors: Mehl Margaret 
Published: January 2008

AbstractObara Kuniyoshi (1887–1977), the founder of Tamagawa Gakuen, and Matsumae Shigeyoshi (1901–1991), the founder of Tôkai Educational System (TES), were both inspired by Denmark and Danish education, although in different ways. Obara, a representative of the New Education Movement in Japan, became interested in Denmark when he heard about Danish gymnastics, which seemed to be ideally suited to his vision of rigorous but non-competitive physical education. In 1931, two years after founding his own school, he succeeded in inviting the Danish gymnast Niels Bukh and a group of his students to Japan. The interest in gymnastics sparked off a wider interest in Denmark. Matsumae Shigeyoshi's attention was drawn to Denmark and to the Danish folk high schools as a result of his encounter with Uchimura Kanzô. Although not an educator by training, he decided to devote his life to education. This article explores the role of cultural borrowing in the thought and educational praxis of Obara and Matsumae. Although they uncritically accepted a Danish cultural memory (Denmark's recovery from military defeat in the nineteenth century through spiritual strength and education) and cultivated a stereotypical image, the adaptation of this image to suit their own needs represents a highly creative process which resulted in two successful private schools.
When the Medium Is the Message: The Ideological Role of Yoshino Sakuzô's Minponshugi in Mobilising the Japanese Public
Authors: McCormick Brett 
Published: January 2008

AbstractDemocratic ideology functioned in Japanese society as part of the national political ethos from 1889 onward. Within the broader ideological discourse, it played a role in conditioning the state-subject relationships that in early Shôwa proved conducive to mass social mobilisation for war. Foremost among the proponents of this democratic ideology was Yoshino Sakuzô, whose democratic theories functioned in practice to promote a sociopolitical hegemony that allowed the imperial state to govern both by coercion and suasion. Yoshino's minponshugi theories constructed a liberalism strictly within a nationalist state-centered theoretical paradigm. Despite negligible functionality, individual participation in social structures like political parties, public political campaigns, and voting, had enduring ideological effect. If ideology within a state interpellates subjects as citizens, like a policeman's hail, 'Hey, you there!' might minponshugi have functioned like the hail from the loudspeaker of a campaign truck in early Shôwa Tokyo as, 'Hey, you there, constituent and voter'?
Regional Integration and Business Interests: A Comparative Study of Europe and Southeast Asia
Authors: Yoshimatsu Hidetaka 
Published: January 2008

AbstractThis article aims to articulate business representation in the process towards creating a unified market by comparing the regional integration process in Europe in the 1980s and in Southeast Asia in the new millennium. Both the European Community (EC) and the Association of Southeast Asian Nations (ASEAN) adopted the creation of a single market as a feasible strategy to respond to an economic hardship. In this process, US business associations played a critical role in identifying problems and issues in promoting actual integration. As for local business, the existing business associations did not function effectively and a new association comprising individual business executives was formed. Importantly, an initiative to create a new association came not from the private sector in Europe but from the member states in Southeast Asia. This difference led to disparities in the relative influence of the two associations on the actual integration processes.
China Through Western Eyes. A Case Study of the BBC Television Documentary Roads to Xanadu
Authors: Cao Qing 
Published: January 2008

AbstractDespite a growing body of literature on the study of the Western media's portrayal of China, little attention has been paid to the structures underlying the representation of China. This essay aims to address this issue through an in-depth analysis of a British television documentary series, Roads to Xanadu (1990). It focuses on dominant perspectives constructed in the series by analysing documentary narrative as a mode of realising discourse. The essay argues that underlying the representation is a technological view of society. Shifting between realpolitik industrialism and liberal democratic humanism, the series attempts to arrive at a unified and unifying version of 'progress' through a modernist discourse.
RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1
Authors: Garnacho Carmen , Shuvaev Vladimir , Thomas Anu , McKenna Lindsay , Sun Jing , Koval Michael , Albelda Steven , Muzykantov Vladimir , Muro Silvia 
Published: January 2008

AbstractPlatelet-endothelial cell adhesion molecule-1 (PECAM-1), a transmembrane glycoprotein involved in leukocyte transmigration, represents a good target for endothelial drug delivery (eg, using antibody-directed nanocarriers, anti-PECAM/NCs). Although endothelial cells do not internalize PECAM antibodies, PECAM-1 engagement by multivalent anti-PECAM conjugates and nanocarriers causes endocytosis via a nonclassic CAM-mediated pathway. We found that endothelial uptake of multivalent anti-PECAM complexes is associated with PECAM-1 phosphorylation. Using model REN cells expressing a series of PECAM-1 deletion and point mutants, we found that the PECAM-1 cytoplasmic domain and, more precisely, PECAM-1 tyrosine 686, is critical in mediating RhoA activation and recruitment of EGFP-RhoA to anti-PECAM/NC binding sites at the plasmalemma, actin polymerization into phalloidin-positive stress fibers, and finally CAM endocytosis of anti-PECAM/NCs. Endothelial targeting and endocytosis of anti-PECAM/NCs were markedly efficient and did not compromise endothelial barrier function in vitro (determined by immunostaining of VE-cadherin and 125I-albumin transport across endothelial monolayers) or in vivo (determined by electron microscopy imaging of pulmonary capillaries and 125I-albumin transport from the blood into the lung tissue after intravenous injection of anti-PECAM/NCs in mice). These results reveal PECAM-1 signaling and interactions with the cytoskeleton, which are required for CAM-endocytosis, and may provide safe intra-endothelial drug delivery by anti-PECAM/NCs.
The Aiolos transcription factor is up-regulated in chronic lymphocytic leukemia
Authors: Duhamel Marianne , Arrouss Issam , Merle-Béral Hélène , Rebollo Angelita 
Published: January 2008

Abstract
The Aiolos transcription factor, member of the Ikaros family of zinc finger proteins, plays an important role in the control of mature B lymphocyte differentiation and proliferation, and its function appears to be modulated through alternative splicing. To assess Aiolos isoform role in humans' pathologies, we studied Aiolos variant distribution and expression in mature B lymphoproliferative disorders (chronic lymphocytic leukemia [CLL] and other B-cell lymphomas). We demonstrated that more than 80% of expressed Aiolos in normal as well as in malignant B cells is of the hAio1 type, and we showed for the first time a homogeneous overexpression of the total amounts of Aiolos transcripts in the B cells of CLL patients, independently of ZAP-70 and IgVH mutational status prognosis factors. This up-regulation of Aiolos, confirmed at protein level, seems independent of Aiolos promoter H3K9 acetylation and H3K4 trimethylation.
Differential production of superoxide by neuronal mitochondria
Authors: Hoegger Mark J , Lieven Christopher J , Levin Leonard A 
Published: January 2008

Abstract

Background
Mitochondrial DNA (mtDNA) mutations, which are present in all mitochondria-containing cells, paradoxically cause tissue-specific disease. For example, Leber's hereditary optic neuropathy (LHON) results from one of three point mutations mtDNA coding for complex I components, but is only manifested in retinal ganglion cells (RGCs), a central neuron contained within the retina. Given that RGCs use superoxide for intracellular signaling after axotomy, and that LHON mutations increase superoxide levels in non-RGC transmitochondrial cybrids, we hypothesized that RGCs regulate superoxide levels differently than other neuronal cells. To study this, we compared superoxide production and mitochondrial electron transport chain (METC) components in isolated RGC mitochondria to mitochondria isolated from cerebral cortex and neuroblastoma SK-N-AS cells.

Results
In the presence of the complex I substrate glutamate/malate or the complex II substrate succinate, the rate of superoxide production in RGC-5 cells was significantly lower than cerebral or neuroblastoma cells. Cerebral but not RGC-5 or neuroblastoma cells increased superoxide production in response to the complex I inhibitor rotenone, while neuroblastoma but not cerebral or RGC-5 cells dramatically decreased superoxide production in response to the complex III inhibitor antimycin A. Immunoblotting and real-time quantitative PCR of METC components demonstrated different patterns of expression among the three different sources of neuronal mitochondria.

Conclusion
RGC-5 mitochondria produce superoxide at significantly lower rates than cerebral and neuroblastoma mitochondria, most likely as a result of differential expression of complex I components. Diversity in METC component expression and function could explain tissue specificity in diseases associated with inherited mtDNA abnormalities.
Usefulness of manufactured tomato extracts in the diagnosis of tomato sensitization: Comparison with the prick-prick method
Authors: Ferrer Ángel , Huertas Ángel J , Larramendi Carlos H , García-Abujeta Jose L , Bartra Joan , Lavín Jose R , Andreu Carmen , Pagán Juan A , López-Matas María A , Fernández-Caldas Enrique , Carnés Jerónimo 
Published: January 2008

AbstractBackgroundCommercial available skin prick test with fruits can be negative in sensitized or allergic patients due to a reduction in biological activity during the manufacturing process. Prick-prick tests with fresh foods are often preferred, but they are a non-standardized procedure. The usefulness of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method in the diagnosis of tomato sensitization has been analyzed.The objective of the study was to assess the potential diagnostic of freeze-dried extracts of Canary Islands tomatoes, comparing the wheal sizes induced by prick test with the prick-prick method.MethodsTwo groups of patients were analyzed: Group I: 26 individuals reporting clinical symptoms induced by tomato contact or ingestion. Group II: 71 control individuals with no symptoms induced by tomato: 12 of them were previously skin prick test positive to a tomato extract, 39 were atopic and 20 were non-atopic. All individuals underwent prick-prick with fresh ripe peel Canary tomatoes and skin prick tested with freeze-dried peel and pulp extracts obtained from peel and pulp of Canary tomatoes at 10 mg/ml. Wheal sizes and prick test positivity (≥ 7 mm2) were compared between groups.ResultsIn group I, 21 (81%) out of 26 patients were prick-prick positive. Twenty patients (77%) had positive skin prick test to peel extracts and 12 (46%) to pulp extracts. Prick-prick induced a mean wheal size of 43.81 ± 40.19 mm2compared with 44.25 ± 36.68 mm2induced by the peel extract (Not significant), and 17.79 ± 9.39 mm2induced by the pulp extract (p < 0.01).In group II, 13 (18%) out of 71 control patients were prick-prick positive. Twelve patients (all of them previously positive to peel extract) had positive skin prick test to peel and 3 to pulp. Prick-prick induced a mean wheal size of 28.88 ± 13.12 mm2compared with 33.17 ± 17.55 mm2induced by peel extract (Not significant), and 13.33 ± 4.80 mm2induced by pulp extract (p < 0.05 with peel extract and prick-prick).ConclusionCanary peel tomato extract seems to be as efficient as prick-prick tests with ripe tomatoes to diagnose patients sensitized to tomato. The wheal sizes induced by prick-prick and peel extracts were very similar and showed a high correlation coefficient.
Roles of adjuvant and route of vaccination in antibody response and protection engendered by a synthetic matrix protein 2-based influenza A virus vaccine in the mouse
Authors: Mozdzanowska Krystyna , Zharikova Darya , Cudic Mare , Otvos Laszlo , Gerhard Walter 
Published: January 2008

AbstractBackgroundThe M2 ectodomain (M2e) of influenza A virus (IAV) strains that have circulated in humans during the past 90 years shows remarkably little structural diversity. Since M2e-specific antibodies (Abs) are capable of restricting IAV replication in vivo but are present only at minimal concentration in human sera, efforts are being made to develop a M2e-specific vaccine. We are exploring a synthetic multiple antigenic peptide (MAP) vaccine and here report on the role of adjuvants (cholera toxin and immunostimulatory oligodeoxynucleotide) and route of immunization on Ab response and strength of protection.ResultsIndependent of adjuvants and immunization route, on average 87% of the M2e-MAP-induced Abs were specific for M2e peptide and a variable fraction of these M2e(pep)-specific Abs (average 15%) cross-reacted with presumably native M2e expressed by M2-transfected cells. The titer of these cross-reactive M2e(pep-nat)-specific Abs in sera of parenterally immunized mice displayed a sigmoidal relation to level of protection, with EC50of ~20 μg Ab/ml serum, though experiments with passive M2e(pep-nat) Abs indicated that serum Abs did not fully account for protection in parenterally vaccinated mice, particularly in upper airways. Intranasal vaccination engendered stronger protection and a higher proportion of G2a Abs than parenteral vaccination, and the strength of protection failed to correlate with M2e(pep-nat)-specific serum Ab titers, suggesting a role of airway-associated immunity in protection of intranasally vaccinated mice. Intranasal administration of M2e-MAP without adjuvant engendered no response but coadministration with infectious IAV slightly enhanced the M2e(pep-nat) Ab response and protection compared to vaccination with IAV or adjuvanted M2e-MAP alone.ConclusionM2e-MAP is an effective immunogen as ~15% of the total M2e-MAP-induced Ab response is of desired specificity. While M2e(pep-nat)-specific serum Abs have an important role in restricting virus replication in trachea and lung, M2e-specific T cells and/or locally produced Abs contribute to protection in upper airways. Intranasal vaccination is preferable to parenteral vaccination, presumably because of induction of local protective immunity by the former route. Intranasal coadministration of M2e-MAP with infectious IAV merits further investigation in view of its potential applicability to human vaccination with live attenuated IAV.
Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India
Authors: Bharaj Preeti , Chahar Harendra S , Pandey Anubhav , Diddi Kavita , Dar Lalit , Guleria Randeep , Kabra Sushil K , Broor Shobha 
Published: January 2008

Abstract

Background
Co-circulation of multiple dengue virus serotypes has been reported from many parts of the world including India, however concurrent infection with more than one serotype of dengue viruses in the same individual is rarely documented. An outbreak of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) occurred in and around Delhi in 2006. This is the first report from India with high percentage of concurrent infections with different dengue virus serotypes circulating during one outbreak.

Results
Acute phase sera from patients were tested for the presence of dengue virus RNA by RT-PCR assay. Of the 69 samples tested for dengue virus RNA, 48 (69.5%) were found to be positive. All the four dengue virus serotypes were found to be co-circulating in this outbreak with DENV-3 being the predominant serotype. In addition in 9 of 48 (19%) dengue virus positive samples, concurrent infection with more than one dengue virus serotype were identified.

Conclusion
This is the first report in which concurrent infections with different dengue virus serotypes is being reported during an outbreak from India. Delhi is now truly hyperendemic for dengue.
Energy balance, leptin, NEFA and IGF-I plasma concentrations and resumption of post partum ovarian activity in swedish red and white breed cows
Authors: Konigsson Kristian , Savoini Giovanni , Govoni Nadia , Invernizzi Guido , Prandi Alberto , Kindahl Hans , Veronesi Maria Cristina 
Published: January 2008

Abstract
In the purpose to provide further information in respect of the relationship between metabolism and post partum (PP) ovarian activity resumption in dairy cows, the aim of the present study was to characterize the energy balance (EB) and leptin, NEFA and IGF-I plasma levels in Swedish Red and White (SRW) cows with and without ovarian activity re-initiation within 7 weeks PP. The study was conducted on 12 primiparous SRW cows fed the same diet as total mixed ration for ad libitum intake. The EB was calculated weekly from parturition until seven weeks PP. Blood samples were collected weekly from one week before until 7 weeks after calving for leptin, NEFA and IGF-I analysis. For progesterone (P4) analysis, blood samples were collected two times per week from parturition until the end of the study. P4 profile was used in addition to the clinical examination to detect cows with and without ovarian activity resumption. The clinical and ultrasonographic examination, coupled with P4 profile analysis showed the resumption of ovarian activity within 7 weeks after calving in 8 (group A) and no ovarian resumption in 4 cows (group B). No significant differences were detected in the whole period of observation in the amount of milk production between the two groups, while the mean milk protein content was significantly lower in group B at the third week PP. The calculated EB was negative in both groups in the first three weeks after calving, but more marked in group B. NEFA and Leptin plasma levels did not show significant differences between the two groups. In conclusion, the results of the present study showed that, when low milk producing primiparous cows are concerned, no significant differences in BW loss, milk yield, EB and leptin and NEFA plasma levels between the cows with and without resumption of ovarian activity within 7 weeks post partum were seen. However, significantly higher IGF-I levels in the first two weeks after calving were found in cows with post partum ovarian activity resumption, highlighting the important role of IGF-I as sensitive signal between metabolism and reproduction.
Identification and Functional Analysis of a New WNT4 Gene Mutation among 28 Adolescent Girls with Primary Amenorrhea and Müllerian Duct Abnormalities: A French Collaborative Study
Authors: Philibert Pascal , Biason-Lauber Anna , Rouzier Roman , Pienkowski Catherine , Paris Françoise , Konrad Daniel , Schoenle Eugene , Sultan Charles 
Published: January 2008

Abstract
Context: Müllerian duct development depends on gene and hormone interactions. Female Wnt4-knockout mice lack müllerian ducts and are virilized due to the inappropriate expression of the enzymes required for androgen production (normally repressed in female ovary). The WNT4 mutation was recently reported to be associated with failure of müllerian duct formation and virilization in two 46, XX women.
Objectives: This collaborative work was designed to determine whether the WNT4 mutation could be identified in a group of adolescent girls with Mayer-Rokitansky-Küster-Hauser syndrome.
Results: We analyzed 28 DNA samples from adolescent girls with primary amenorrhea and failure of müllerian duct formation by direct sequencing and identified a new L12P mutation within exon 1 of the WNT4 gene. The substitution of leucine by proline is crucial for the conformation of the expressed protein. This amino acid substitution is unlikely to be a polymorphism because it was not found in 100 DNAs from control subjects. Functional analysis revealed that the mutation induces significantly increased expression of the enzymes involved in androgen biosynthesis (3β-hydroxysteroid dehydrogenase and 17α-hydroxylase). It is interesting to note that the adolescent carrying the mutation was referred to our clinic for primary amenorrhea and hyperandrogenism (severe acne and plasma testosterone: 1.8 vs. 1.2 nmol/liter in controls). She also presented with uterine hypoplasia and follicle depletion.
Conclusions: We suggest that in adolescent girls with primary amenorrhea, müllerian duct abnormalities, and hyperandrogenism, a WNT4 mutation should be sought. Moreover, our data confirm that WNT4 is involved in the regulation of müllerian duct development and ovarian androgen biosynthesis. WNT4 may also contribute to human follicle development and/or maintenance.