bluuebunny/crossref_metadata_2025_split huggingface dataset:
Updating Nonlinear Stochastic Dynamics of an Uncertain Nozzle Model Using Probabilistic Learning With Partial Observability and Incomplete Dataset
Authors: Capiez-Lernout Evangéline , Ezvan Olivier , Soize Christian 
Published: April 2024

Abstract
This article introduces a methodology for updating the nonlinear stochastic dynamics of a nozzle with uncertain computational model. The approach focuses on a high-dimensional nonlinear computational model constrained by a small target dataset. Challenges include the large number of degrees-of-freedom, geometric nonlinearities, material uncertainties, stochastic external loads, underobservability, and high computational costs. A detailed dynamic analysis of the nozzle is presented. An updated statistical surrogate model relating the observations of interest to the control parameters is constructed. Despite small training and target datasets and partial observability, the study successfully applies probabilistic learning on manifolds (PLoM) to address these challenges. PLoM captures geometric nonlinear effects and uncertainty propagation, improving conditional mean statistics compared to training data. The conditional confidence region demonstrates the ability of the methodology to accurately represent both observed and unobserved output variables, contributing to advancements in modeling complex systems.
Hole Edge Metrology and Inspection by Edge Diffractometry
Authors: Lu Kuan , Lee ChaBum 
Published: April 2024

Abstract
This article introduces a novel hole edge inspection and metrology technology by edge diffractometry, which occurs when light interacts with the hole edge. The proposed method allows for simultaneous characterization of hole part error and edge roughness conditions. Edge diffraction occurs as light bends at a sharp edge. Such a diffractive fringe pattern, the so-called interferogram, is directly related to edge geometry and roughness. Image-based diffractometry inspection technology was developed to capture the diffractive fringe patterns. The collected fringe patterns were analyzed through statistical feature extraction methods, and numerical results such as roundness index, concentricity, and via edge roughness (VER) were obtained. The results indicated that hole 1 had an average VER of 0.665 μm and a roundness index of 0.95, while hole 2 was measured an average VER of 0.753 μm and a roundness index of 0.96. Through-focus scanning optical microscopy (TSOM) was also utilized to perform three-dimensional characterization of hole features along the depth direction. As a result, the proposed method could characterize hole part error and evaluate its roughness conditions. This study showed the potential to be adapted for automatic optical inspection for advancing microelectronics and semiconductor packaging technology.
Evaluation and activity of new porphyrin-peptide cage-type conjugates for the photoinactivation of
Mycobacterium abscessus
Authors: Alcaraz Matthéo , Lyonnais Sébastien , Ghosh Chandramouli , Aguilera-Correa John Jairo , Richeter Sébastien , Ulrich Sébastien , Kremer Laurent 
Published: April 2024

ABSTRACT

Mycobacterium abscessus
is increasingly recognized as an emerging opportunistic pathogen causing severe lung diseases and cutaneous infections. However, treatment of
M. abscessus
infections remains particularly challenging, largely due to intrinsic resistance to a wide panel of antimicrobial agents. New therapeutic alternatives are urgently needed. Herein, we show that, upon limited irradiation with a blue-light source, newly developed porphyrin-peptide cage-type photosensitizers exert a strong bactericidal activity against smooth and rough variants of
M. abscessus
in planktonic cultures and in biofilms, at low concentrations. Atomic force microscopy unraveled important morphological alterations that include a wrinkled and irregular bacterial surface. The potential of these compounds for a photo-therapeutic use to treat
M. abscessus
skin infections requires further evaluations.

IMPORTANCE

Mycobacterium abscessus
causes persistent infections and is extremely difficult to eradicate. Despite intensive chemotherapy, treatment success rates remain very low. Thus, given the unsatisfactory performances of the current regimens, more effective therapeutic alternatives are needed. In this study, we evaluated the activity of newly described porphyrin-peptide cage-type conjugates in the context of photodynamic therapy. We show that upon light irradiation, these compounds were highly bactericidal against
M. abscessus in vitro
, thus qualifying these compounds for future studies dedicated to photo-therapeutic applications against
M. abscessus
skin infections.
Novel mutation leading to splice donor loss in a conserved site ofDMDgene causes Duchenne muscular dystrophy with cryptorchidism
Authors: Chen Jianhai , Jia Yangying , Zhong Jie , Zhang Kun , Dai Hongzheng , He Guanglin , Li Fuping , Zeng Li , Fan Chuanzhu , Xu Huayan 
Published: April 2024

BackgroundAs one of the most common congenital abnormalities in male births, cryptorchidism has been found to have a polygenic aetiology according to previous studies of common variants. However, little is known about genetic predisposition of rare variants for cryptorchidism, since rare variants have larger effective size on diseases than common variants.MethodsIn this study, a cohort of 115 Chinese probands with cryptorchidism was analysed using whole-genome sequencing, alongside 19 parental controls and 2136 unaffected men. Additionally, CRISPR-Cas9 editing of a conserved variant was performed in a mouse model, with MRI screening used to observe the phenotype.ResultsIn 30 of 115 patients (26.1%), we identified four novel genes (ARSH,DMD,MAGEA4andSHROOM2) affecting at least five unrelated patients and four known genes (USP9Y,UBA1,BCORL1andKDM6A) with the candidate rare pathogenic variants affecting at least two cases. Burden tests of rare variants revealed the genome-wide significances for newly identified genes (p<2.5×10−6) under the Bonferroni correction. Surprisingly, novel and known genes were mainly found on X chromosome (seven on X and one on Y) and all rare X-chromosomal segregating variants exhibited a maternal inheritance rather than de novo origin. CRISPR-Cas9 mouse modelling of a splice donor loss variant inDMD(NC_000023.11:g.32454661C>G), which resides in a conserved site across vertebrates, replicated bilateral cryptorchidism phenotypes, confirmed by MRI at 4 and 10 weeks. The movement tests further revealed symptoms of Duchenne muscular dystrophy (DMD) in transgenic mice.ConclusionOur results revealed the role of theDMDgene mutation in causing cryptorchidism. The results also suggest that maternal-X inheritance of pathogenic defects could have a predominant role in the development of cryptorchidism.
NixFe100-x for urea and oxygen evolution: a matter of compromise
Authors: Ruscica Noah , Clark Rylan , Stuart Jordan , Mason Aaron , Bennett Craig , Bertin Erwan 
Published: April 2024

The combination of aqueous electrolysis, either for hydrogen generation or CO2 conversion, with wastewater treatment offers an elegant way to tackle issues associated with our energy transition and the need for clean drinking water. However, it requires an anode capable of doing both the oxidation of the targeted pollutant and the oxygen evolution reaction (OER), as most pollutants are present in too low concentration to be practical for industrial electrolysis. In this study, we focussed on the oxidation of urea on NixFe100-x catalysts. These catalysts were prepared by pulsed laser ablation in liquid, a versatile and green technique to prepare electrocatalysts. Transmission electron microscopy of the nanoparticles indicates the production of monodisperse nanoparticles, with an average diameter increasing from 7.8 ± 2.8 to 19.7 ± 3.9 nm with a higher iron fraction. The composition could be controlled between pure Ni and NiFe bimetallic nanoparticles with up to 56 ± 3% of iron, by controlling the composition of the target. A brief optimisation of the electrode preparation (loading, catalyst-to-carbon ratio) yielded an optimum at about 30 µg/cm2 of catalyst with a catalyst-to-carbon ratio of 20:80. During the electrocatalytic tests, Ni was the best catalyst for urea oxidation, with a maximum peak current of 619 mA/mg. However, Ni75Fe25 was the best OER catalyst, showing a peak current of 1150 mA/mg. The difference increased further during CA at 0.5 V, during which Ni75Fe25 outperformed pure Ni by almost a factor of 3 after 30 min.
Geochronology, geochemistry, and Hf isotope of quartz monzonite and diorite in dachaigou, the East Kunlun orogenic belt, China, and their geological significance
Authors: Sun Yaming , Li Bile , Li Peng , Li Chengxue , Li Zhihua , Shi Yufan 
Published: April 2024

This study focuses on quartz monzonite and diorite plutons in Dachaigou, East Kunlun, examining their formation, age, petrogenesis, magmatic source, and tectonic setting. Through comprehensive field observations, zircon geochronology, geochemistry, and Hf isotope analysis, new insights into the Paleozoic tectonic evolution of the proto-Tethys Ocean in the East Kunlun orogenic belt are provided. Zircon U–Pb dating reveals that quartz monzonite and diorite formed at 417.4 ± 2.1 and 404.6 ± 1.8 Ma, respectively. Geochemical analysis shows that the A/CNK value of quartz monzonite ranges from 0.98 to 1.07, with the Pb content proportional to SiO2, which aligns with the characteristics of I-type granite. The εHf(t) values for quartz monzonite and diorite are −0.84 to 2.2 and −13.22 to 2.04, respectively. Considering the formation age, geochemical characteristics, and regional tectonic evolution, it is concluded that quartz monzonite has a crust-derived origin, whereas diorite has a crust–mantle mixing origin. Both were formed in a post-collision extensional tectonic setting resulting from slab break-off, implying the Proto-Tethys Ocean East Kunlun entered a post-collisional extension stage in the Late Silurian–Early Devonian.
Mating disruption alters the rate of development in an aphidophagous ladybird Propylea dissecta (Mulsant)
Authors: Gupta Dipali , Mishra Geetanjali 
Published: April 2024

Ladybird beetles like many other organisms show developmental rate polymorphism in response to various abiotic and biotic factors. Since mating disruption acts as a stressor, we decided to study its impact on the occurrence of developmental rate polymorphs in ladybird beetle, Propylea dissecta Mulsant, 1850 (Coleoptera: Coccinellidae). Ten-day-old females were subjected to mating disruptions at 120, 180 min, and complete mating (211 ± 8.1 min) and then provided with optimum diet of Aphis craccivora Koch. Larvae in each treatment were reared individually and newly emerged adults were assessed on the basis of their developmental durations. They were separated into two lines: slow and fast developers. Extended mating duration was found to be correlated to increased immature survival and reduced offspring development times. Also, in complete mating treatment, percentage of fast developers were more in numbers; thus, mating duration increased the availability of sperm for females and seminal fluid may have boosted maternal protein reserves, which can then be used to produce offspring of better quality. Mating disruption might be a factor reducing the chances of fertilization success and offspring development in P. dissecta.
Thyroid and contralateral breast surface dose variation in mammography: a phantom study on the role of breast tissue composition
Authors: Ekinci Fatih , Aşlar Engin 
Published: April 2024

AbstractThe organs of the thyroid and contralateral breast can be exposed to radiation because of scattered x-rays during mammography. It is important to determine the dose values of these quantities in terms of the risk of triggering a second cancer induction for both organs. In the present study, thyroid and contralateral breast surface doses were investigated with LiF:Mg,Ti (TLD-100) dosimeters for three types of BR-12 phantoms with glandularity/fat tissue ratios (70%/30%, 50%/50% and 30%/70%) over four views as in the real patient situation in Mo/Mo and Mo/Rh anode/filter combinations in each phantom thickness as opposed to a single glandular/fat tissue commonly used in the literature. Both thyroid and contralateral surface doses increased with increasing both phantom thickness and glandularity/fat ratio. The thyroid surface doses changed within 0.06–0.18 mGy and 0.05–0.14 mGy according to phantom thickness of 2 to 6 cm for the glandularity/adipose of 50%/50% in the Mo/Mo and Mo/Rh, respectively. On the other hand, the contralateral breast surface doses were within 0.35–1.39 mGy and 0.40–0.99 mGy for 50%/50% in the Mo/Mo and Mo/Rh, respectively. Based on a 70%/30% breast composition with a phantom thickness of 6 cm, the thyroid and contralateral breast surface doses increased by approximately 40% compared with 50%/50% for both Mo/Mo and Mo/Rh. These results showed that both thyroid and contralateral breast surface doses significantly depend on the glandularity/fat composition of the breast. Therefore, the outputs of this study may contribute to future studies aimed at reducing the doses received by organs during examination.
Unveiling nuclear isomers through multiple-reflection time-of-flight mass spectrometry
Authors: Dickel Timo , Mollaebrahimi Ali 
Published: April 2024

AbstractNuclear isomers, the excited meta-stable states of nuclei, offer profound insights into nuclear structure. This article reviews the intersection of nuclear isomer research with mass spectrometry methodologies, particularly focusing on novel capabilities of the multiple-reflection time-of-flight mass spectrometry (MR-TOF-MS) technique. Through a comprehensive examination of established methods for isomer identification and characterization, alongside the technical principles underlying MR-TOF-MS, this review discusses the pivotal role of mass spectrometry in advancing our understanding of nuclear isomers. The operational principles and recent developments in MR-TOF-MS technology are explained and exemplified through case studies from prominent research facilities. Furthermore, this work discusses ongoing efforts to enhance sensitivity, resolution, and measurement capabilities in MR-TOF-MS, promising continued advancements in nuclear physics research and applications.
Order-Guided Non-Conservative Maximum Flow in Uncertain Network Interdiction Problem with Budget Constraint
Authors: Pangeni Badri Prasad , Dhamala Tanka Nath 
Published: April 2024

Probability theory in decision science fails in the absence of sufficient data. In these situations, uncertainty theory is applied to the problem-solving process, taking into account the opinions of the domain experts. In order to minimize the maximum flow carried by the evader, we describe an interdiction problem in an uncertain network with intermediate storage and resource constraints. Such approaches would help law enforcement combat drug-related and smuggling activities. This work first converts the bi-level deterministic conservative model of the interdictor vs evader problem (IEP) into a non-conservative deterministic IEP. With intermediate storage, a binary interdiction variable, and an interdiction resource constraint, the model is further transformed into an uncertain measure-based model. Then, utilizing uncertainty theory, the uncertain model is again converted into its deterministic equivalent. We have presented two techniques to identify the arc that the interdictor needs to block and to direct the order-guided maximum flow that the evader needs to deliver to the target. The models’ and algorithms’ efficacy is confirmed by an example. Lastly, a graphic representation of the shifting trend of the value of the objective function (VOF) is shown for a range of confidence levels.
Supplementary Figure 9 from CB307: A Dual Targeting Costimulatory Humabody V<sub>H</sub> Therapeutic for Treating PSMA-Positive Tumors
Authors: Archer Sophie , Brailey Phillip M. , Song Minjung , Bartlett Phillip D. , Figueiredo Ines , Gurel Bora , Guo Christina , Brucklacher-Waldert Verena , Thompson H. Lorraine , Akinwale Jude , Boyle Samantha E. , Rossant Christine , Birkett Neil R. , Pizzey Julia , Maginn Mark , Legg James , Williams Richard , Johnston Colette M. , Bland-Ward Philip , de Bono Johann S. , Pierce Andrew J. 
Published: April 2024

<p>Supplementary Figure 9: Neuroendocrine Differentiation Phenotypes in Prostate Cancer Liver Metastases</p>
Abstract P45: REDUCED IMMUNOGENICITY DRIVES SUPERIORITY OF THE NANOLUCIFERASE (NLUC) REPORTER IN AN IMMUNOCOMPETENT GL261 MOUSE GLIOMA MODEL
Authors: Victorio Carla Bianca Luena , Novera Wisna , Ganasarajah Arun , Ong Joanne , Li Peter , Petersen Sven Hans , Msallam Rasha , Chacko Ann-Marie 
Published: April 2024

Abstract
Bioluminescence imaging (BLI) with luciferase-based reporters is a robust approach for monitoring tumour growth and treatment response in preclinical in vivo cancer models. Luciferases are considered foreign molecules that could elicit immune responses when introduced in immunocompetent live animal models [1, 2]. The immunogenicity of luciferases and other transgenic imaging reporters, though often overlooked, was recently discussed in relation to mouse cancer models [3-5]. Herein, we investigated the immunogenicity of two luciferase reporters: (1) red-shifted firefly luciferase (RFLuc, 61 kDa) and (2) deep-sea shrimp nanoluciferase (nLuc, 19 kDa) in an orthotopic brain tumour model using GL261 mouse glioma cells in a syngeneic C57BL/6J mouse. We evaluated their suitability for monitoring of brain tumour growth kinetics by BLI. Mice were implanted with 2 × 104 either GL261, GL261-NLuc or GL261-RFLuc cells in the right cerebrum using a stereotaxic device. GL261-RFLuc tumours could only be detected by BLI for 2 weeks, and the mice did not die of disease (median overall survival; mOS &gt; 35 days). In contrast, GL261-nLuc cells formed lethal brain tumours that grew in volume as determined by BLI. GL261-NLuc implanted mice exhibited mOS comparable to mice implanted with GL261 cells (24.5 days vs. 23 days). These observations indicated that nLuc is not significantly immunogenic in mouse brains and did not cause rejection of the tumour cells as observed with RFluc. Cytokine expression profiles and immune cell profiles of the tumour-bearing brain hemispheres were comparable between tumours from GL261 and GL261-NLuc. In contrast, GL261-RFLuc tumours exhibited elevated CD8+ T cells, particularly effector (CD62L¯CD44+) and granzyme B-expressing (GZB+) CD8+ T cells. GL261-nLuc formed stable glioma tumors comparable to the original GL261 mouse model and is a suitable in vivo preclinical model for evaluating experimental therapeutic interventions against gliomas, when paired with Promega’s proprietary fluoro-furimazine substrate.
Funding: This research was supported by Singapore’s Health and Biomedical Sciences (HBMS) Industry Alignment Fund Pre-Positioning (IAF-PP) grant H18/01/a0/018 (to AMC) administered by A*STAR and Singapore-MIT Alliance for Research and Technology (SMART) Innovation Grant (ING-000913 BIO) (to AMC).
Citation Format: Carla Bianca Luena Victorio, Wisna Novera, Arun Ganasarajah, Joanne Ong, Peter Li, Sven Hans Petersen, Rasha Msallam, Ann-Marie Chacko. REDUCED IMMUNOGENICITY DRIVES SUPERIORITY OF THE NANOLUCIFERASE (NLUC) REPORTER IN AN IMMUNOCOMPETENT GL261 MOUSE GLIOMA MODEL [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr P45.
Abstract P49: PROTAC A1874: A Potential Targeted Therapy for Breast Cancer
Authors: Marvalim Charlie , Datta Arpita , Seah Serena Bee Kee , Chow Edward Kai-Hua , Lee Soo Chin 
Published: April 2024

Abstract
Background
PROteolysis TArgeting Chimera (PROTAC) A1874 is a heterobifunctional structured compound consisting of a BRD4-recognizing ligand and an MDM2-recognizing ligand that functions to degrade BRD4 via the ubiquitin-proteasome system. A1874 also plays a dual role in downregulating downstream BRD4 regulator, MYC, and stabilizing p53 within nanomolar concentrations. This PROTAC thus presents itself as a potential therapy for both estrogen-receptor positive (ER+) and triple-negative breast cancer (TNBC) subtypes, which typically harbors wild-type p53 and are associated with MYC overexpression respectively. Thus, this study aims to 1) investigate the anti-oncogenic effects and mechanism of A1874 and 2) its effect in combination with existing therapies in several breast cancer (BC) cell lines.
Methods
A panel of BC cell lines harboring different p53 status were tested for their sensitivity to A1874. Several functional assays were conducted to determine the anti-oncogenic effects of A1874. Bliss scores were calculated using SynergyFinder to determine the interaction of drugs in combination with A1874.
Results
BC cell lines with p53 WT status showed significantly lower IC50 sensitivity to A1874 in the nanomolar range compared to those with p53 mutation, which exhibited sensitivity in the micromolar range regardless of subtypes. Moreover, it is effective in Tam-resistant ER+, p53 WT cells. A1874 exclusively degrades BRD4 in p53 WT cells and causes G1 arrest, inhibition of colony formation, proliferation, and migration. Knockdown of MDM2 or TP53, and lentiviral transduction of p53 mutant plasmid resulted in significant reduction in sensitivity to A1874 in p53 WT cell lines and abrogate its BRD4-degrading effect. Combination of A1874 with the mutant p53 reactivating drug, PRIMA-1, synergistically resensitize mutant p53 cells to the cytotoxic effect of A1874 across subtypes.
Conclusions
Our results highlight a promising potential of PROTAC A1874 as a therapeutic drug in breast cancer, specifically the ER+ subtype which typically retains its p53 WT status.
Citation Format: Charlie Marvalim, Arpita Datta, Serena Bee Kee Seah, Edward Kai-Hua Chow, Soo Chin Lee. PROTAC A1874: A Potential Targeted Therapy for Breast Cancer [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr P49.
IDENTIFICATION OF MUC1-C AS A TARGET FOR SUPPRESSING PROGRESSION OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS
Authors: Nakashoji Ayako , Haratake Naoki , Bhattacharya Atrayee , Mao Weipu , Xu Kangjie , Wang Keyi , Daimon Tatsuaki , Ozawa Hiroki , Shigeta Keisuke , Fushimi Atsushi , Yamashita Nami , Morimoto Yoshihiro , Shimokawa Mototsugu , Saito Shin , Egloff Ann Marie , Uppaluri Ravindra , Long Mark D , Kufe Donald 
Published: April 2024

Abstract
The MUC1-C protein is aberrantly expressed in adenocarcinomas of epithelial barrier tissues and contributes to their progression. Less is known about involvement of MUC1-C in the pathogenesis of squamous cell carcinomas (SCCs). Here, we report that the MUC1 gene is upregulated in advanced head and neck SCCs (HNSCCs). Studies of HNSCC cell lines demonstrate that the MUC1-C subunit regulates expression of (i) RIG-I and MDA5 pattern recognition receptors, (ii) STAT1 and interferon (IFN) regulatory factors, and (iii) downstream IFN-stimulated genes (ISGs). MUC1-C integrates chronic activation of the STAT1 inflammatory pathway with induction of the ∆Np63 and SOX2 genes that are aberrantly expressed in HNSCCs. In extending those dependencies, we demonstrate that MUC1-C is necessary for NOTCH3 expression, self-renewal capacity and tumorigenicity. The findings that MUC1 associates with ∆Np63, SOX2 and NOTCH3 expression by scRNA-seq analysis further indicate that MUC1-C drives the HNSCC stem cell state and is a target for suppressing HNSCC progression.