bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deletion of the citron kinase gene selectively affects the number and distribution of interneurons in barrelfield cortex
Authors: Muzzi Patrizia , Camera Paola , di Cunto Ferdinando , Vercelli Alessandro 
Published: February 2009

AbstractCitron kinase (CIT‐K), a ser/thr kinase, is required during neurogenesis for cytokinesis of neuronal precursors. Deletion of the cit‐k gene in mice (cit‐k−/− mice) leads to a severe malformative central nervous system syndrome characterized by microencephaly, ataxia, and epileptic seizures; affected mice die by the third week of postnatal life. We have used NADPH‐diaphorase histochemistry, immunostaining for calbindin, calretinin, parvalbumin, and glutamic acid decarboxylase 67 (GAD67), and histological staining to undertake qualitative and quantitative analyses of the morphology and distribution of interneurons in the barrelfield cortex of cit‐k−/− mice. By postnatal day 13, lack of CIT‐K results in profoundly altered cortical cell morphology: the infragranular layers are populated by large, binucleate interneurons bearing many more dendrites than in control mice, an anatomical profile that has also been reported for the cortex of humans with cortical dysplasias and epilepsy. Tessellation analyses reveal that a clustered distribution of interneurons is maintained in cit‐k−/− mice, but that their nearest neighbor distance is significantly increased, and thus their density is reduced; the overall number of interneurons is more dramatically decreased in the absence of CIT‐K than would be predicted on the basis of the reduced brain size of affected mice. This reduction of inhibitory γ‐aminobutyric acid (GABA)ergic neurons likely underlies the occurrence of epileptic seizures in the cit‐k−/− mice. Furthermore, the altered distribution of NADPH‐diaphorase‐positive interneurons could be responsible for an impaired coupling of cortical activity to blood flow, also affecting cortical growth and functioning. J. Comp. Neurol. 513:249–264, 2009. © 2009 Wiley‐Liss, Inc.
Fabrication of Monodisperse Toroidal Particles by Polymer Solidification in Microfluidics
Authors: Wang Baoguo , Shum Ho Cheung , Weitz David A. 
Published: February 2009

AbstractMicrodoughnuts: Polymer toroidal particles such as the one shown in the left picture have been prepared by a capillary microfluidic technique. Droplets of polymer solution undergo non‐uniform solidification to form the anisotropic polymer particles. By incorporating functional materials inside the polymer network, functional toroidal particles (center and right images) can be tailor‐made for specific applications such as magnetic actuation.magnified image
Differentiating between endocervical glandular neoplasia and high grade squamous intraepithelial lesions in endocervical crypts: Cytological features in ThinPrep and SurePath cervical cytology samples
Authors: Thiryayi Sakinah A. , Marshall Janet , Rana Durgesh N. 
Published: February 2009

AbstractA recent audit at our institution revealed a higher number of cases diagnosed as endocervical glandular neoplasia on ThinPrep (TP) cervical cytology samples (9 cases) as opposed to SurePath (SP) (1 case), which on histology showed only high‐grade cervical intraepithelial neoplasia (CIN) with endocervical crypt involvement (CI). We attempted to ascertain the reasons for this finding by reviewing the available slides of these cases, as well as slides of cases diagnosed as glandular neoplasia on cytology and histology; cases diagnosed as high‐grade squamous intraepithelial lesions (HSIL) on cytology which had CIN with CI on histology and cases with mixed glandular and squamous abnormalities diagnosed both cytologically and histologically. Single neoplastic glandular cells and short pseudostratified strips were more prevalent in SP than TP with the cell clusters in glandular neoplasia 3–4 cells thick, in contrast to the dense crowded centre of cell groups in HSIL with CI. The cells at the periphery of groups can be misleading. Cases with HSIL and glandular neoplasia have a combination of the features of each entity in isolation. The diagnosis of glandular neoplasia remains challenging and conversion from conventional to liquid based cervical cytology requires a period of learning and adaptation, which can be facilitated by local audit and review of the cytology slides in cases with a cytology–histology mismatch. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.
Fine‐needle cytology of metastatic endometrioid neoplasms: Experience with eight cases
Authors: Fulciniti Franco , Losito Nunzia Simona , Botti Gerardo , Pignata Sandro , Pisano Carmela , Kobayashi Tadao K. , Laurelli Giuseppe , Greggi Stefano 
Published: February 2009

AbstractDespite the frequency of endometrioid malignancies, few articles in the literature are found concerning their cytopathologic presentation on fine‐needle cytology samples. This report describes the cytomorphological findings in eight cases of recurrent or metastatic endometrioid neoplasms on fine‐needle cytology samples obtained from various body sites. The cytological findings in metastatic or recurrent endometrioid carcinomas could be classified into five main patterns (i.e.: endometrioid, adeno‐squamous, villo‐glandular, clear cell, and papillary‐serous), in analogy to histology. It is the authors' feeling that an adequate knowledge of the cytopathological features of this group of neoplasms may be important in favoring an early detection of their relapses or metastases and may contribute to save diagnostic time and more invasive procedures to the patients. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.
Differentiating reactive mesothelial cells from metastatic adenocarcinoma in serous effusions: The utility of immunocytochemical panel in the differential diagnosis
Authors: Saleh Husain A. , El‐Fakharany Mohammad , Makki Hassan , Kadhim Ahmad , Masood Shahla 
Published: February 2009

AbstractDifferentiating reactive mesothelial cells (RMs) from metastatic adenocarcinoma cells (MAC) in serous fluids based on cytomorphologic features alone can be very challenging. Various immunocytochemical (ICC) markers have been used to maximize the diagnostic accuracy, however, cytopathologists still encounter difficulties in effusion cytologic diagnosis. The aim of this study was to evaluate previous and recent ICC stains to identify the most sensitive and specific markers and the best panel for differentiating RM from MAC.Cell block sections from 41 MAC and 43 RM effusions cases were subjected to ICC staining for MOC‐31, BerEp4, carcinoembryonic antigen (CEA), calretinin, HBME‐1, CK5/6, and D2‐40. For the MAC cases, the sensitivity of BerEp4, MOC‐31, and CEA was 82.9, 92.6, and 17%, respectively, and the specificity was 95.3, 93, and 100%, respectively. For the RM cases, the sensitivity of calretinin, CK5/6, D2‐40, and HBME‐1 was 95.3, 27.9, 58.1, and 93%, respectively, and the specificity was 70.7, 73.1, 75.6, and 82.9%, respectively. The results show that BerEp4 and MOC‐31 are highly sensitive and specific for detecting MAC, whereas calretinin and HBME1 are highly sensitive but only modestly specific for detecting RM cases (P < 0.05). Forced entry logistic regression revealed that using MOC‐31, BerEp4, HBME‐1, and calretinin, is an excellent panel for making correct diagnosis with 97.6% sensitivity in detecting MAC and 90.7% specificity in detecting RM.We conclude that adding a panel of MOC‐31, BerEp4, calretinin, and HBME‐1 immunostains to routine cytomorphologic features can greatly enhance the diagnostic accuracy of serous effusions. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.
Informationen aus dem Zulassungsbereich Brandschutz
Authors: Bolze Maja 
Published: February 2009

AbstractAus gegebenem Anlass möchte das Deutsche Institut für Bautechnik (DIBt) im Folgenden nähere Erläuterungen zu einigen speziellen Zulassungsbereichen geben:1. Zulassungsbereich “Feuerschutzabschlüsse”2. Zulassungsbereich “Absperrvorrichtungen gegen Feuer und Rauch in Lüftungsleitungen”3. Zulassungsbereich “Feuerwiderstandsfähige Abschlüsse besonderer Bauart und Verwendung”
New meaning in the message: Noncoding RNAs and their role in retinal development
Authors: Rapicavoli Nicole A. , Blackshaw Seth 
Published: February 2009

AbstractRecent studies have indicated that non‐protein‐coding RNAs (ncRNAs) may play prominent and diverse roles in the development of the nervous system. These ncRNAs are now known to perform a broad range of cellular functions, and in particular appear to be prominent players in the regulation of transcription and translation. In this review, we discuss recent advances in our understanding of the role of ncRNAs in vertebrate retinal development. Noncoding RNAs that are known or suspected to play a functional role in the specification and maturation of retinal cell subtypes include miRNAs, long noncoding opposite‐strand transcripts (OSTs), and other long ncRNAs such as Tug1 and RNCR2. Though the mechanism of action of most of these ncRNAs is still largely unclear, it is likely that these molecules represent a major, and thus far largely unappreciated, component of the molecular machinery involved in retinal cell fate specification. Developmental Dynamics 238:2103–2114, 2009. © 2009 Wiley‐Liss, Inc.
Deletion of a conserved noncoding sequence in Plzf intron leads to Plzf down‐regulation in limb bud and polydactyly in the rat
Authors: Liška František , Šnajdr Pavel , Šedová Lucie , Šeda Ondřej , Chylíková Blanka , Slámová Petra , Krejčí Eliška , Sedmera David , Grim Miloš , Křenová Drahomíra , Křen Vladimír 
Published: February 2009

AbstractLx mutation in SHR.Lx rat manifests in homozygotes as hindlimb preaxial polydactyly. It was previously mapped to a chromosome 8 segment containing the Plzf gene. Plzf (promyelocytic leukemia zinc finger protein) influences limb development as a direct repressor of posterior HoxD genes. However, the Plzf coding sequence is intact in the Lx mutants. Using linkage mapping in F2 hybrids, we downsized the segment containing Lx to 155 kb and sequenced conserved noncoding elements (CNEs) inside. A 2,964‐bp deletion in Plzf intron 2, never detected in control animals, is the only candidate for Lx. The deletion removes the most deeply conserved CNE in the 155‐kb segment, suggesting a regulatory influence on Plzf expression. Correspondingly, using in situ hybridization and quantitative real‐time polymerase chain reaction, we found a decrease of Plzf expression in Lx/Lx limb buds with concomitant anterior expansion of expression domains of its targets, Hoxd10–13 genes, in the absence of ectopic Sonic hedgehog expression. Upstream regulation of Plzf in limb buds is currently unknown. We present here the first candidate Plzf cis‐regulatory sequence. Developmental Dynamics 238:673–684, 2009. © 2009 Wiley‐Liss, Inc.
Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafish
Authors: Ogura Eri , Okuda Yuichi , Kondoh Hisato , Kamachi Yusuke 
Published: February 2009

AbstractAn enhancer trap‐based GAL4‐UAS system in zebrafish requires strong GAL4 activators with minimal adverse effects. However, the activity of yeast GAL4 is too low in zebrafish, while a fusion protein of the GAL4 DNA‐binding domain and the VP16 activation domain is toxic to embryonic development, even when expressed at low levels. To alleviate this toxicity, we developed variant GAL4 activators by fusing either multimeric forms of the VP16 minimal activation domain or the NF‐κB activation domain to the GAL4 DNA‐binding domain. These variant GAL4 activators are sufficiently innocuous and yet highly effective transactivators in developing zebrafish. Enhancer‐trap vectors containing these GAL4 activators downstream of an appropriate weak promoter were randomly inserted into the zebrafish genome using theSleeping Beautytransposon system. By the combination of these genetic elements, we have successfully developed enhancer trap lines that activate UAS‐dependent reporter genes in a tissue‐specific fashion that reflects trapped enhancer activities. Developmental Dynamics 238:641–655, 2009. © 2009 Wiley‐Liss, Inc.
Anatomical location of mature GnRH neurons corresponds with their birthdate in the developing mouse
Authors: Jasoni Christine L. , Porteous Robert W. , Herbison Allan E. 
Published: February 2009

AbstractThe gonadotropin‐releasing hormone (GnRH) neurons exhibit substantial functional, anatomical, and molecular heterogeneity, which has hampered their thorough examination. This study was undertaken in an effort to understand whether the anatomical distribution of GnRH neurons is related to their developmental history, because such an association may help explain differences within the population. Using bromodeoxyuridine pulse labeling of timed pregnant female mice we labeled dividing cells, including GnRH neuron progenitors in the olfactory placode, throughout the window of GnRH neuron differentiation. Our results indicate that cells that become postmitotic early tend to populate the rostral aspects of the adult GnRH neuron continuum, whereas later‐generated cells tend to settle more caudally; an inside‐out pattern reminiscent of neocortex. These observations suggest that the timing of differentiation influences the ability of postmitotic GnRH neurons to navigate to their adult location, and hence may be important in determining the ultimate wiring of the adult network. Developmental Dynamics 238:524–531, 2009. © 2009 Wiley‐Liss, Inc.
Examining requirement for formation of functional Presenilin proteins and their processing events in vivo
Authors: Barakat Ala , Mercer Bettie , Cooper Emilie , Chung Hui‐Min 
Published: February 2009

AbstractPresenilin (Psn) is a multipass transmembrane protein that functions as the catalytic subunit of γ‐secretase for mediating intramembrane cleavage of type 1 transmembrane proteins. Normally active Psn is in the form of a heterodimer composed by its N‐terminal and C‐terminal fragments that are generated from a Presenilinase‐mediated endoproteolytic cleavage within its large cytosolic loop during assembly of the protease complex. Using the Psn forms that either bypass or disable Presenilinase‐mediated endoproteolysis, and a Psn form that has most of the large cytosolic loop deleted, we have established an in vivo system to enable investigations of Psn functional domains in Drosophila. We show that the Presenilinase‐mediated endoproteolytic event is not essential for producing Psn activity during animal development, and is regulated by integrity of the large cytosolic loop of Psn in Drosophila. The Psn transgenic flies described here could be applied to a broad range of studies on Psn functioning and its related γ‐secretase activity at any developmental stage. genesis 47:161–168, 2009. © 2009 Wiley‐Liss, Inc.
Hybrid camera surveillance system using robust human detection
Authors: Iwata Kenji , Satoh Yutaka , Yoda Ikushi , Sakaue Katsuhiko 
Published: February 2009

AbstractWe propose a novel surveillance system that uses a hybrid camera network. The system contains a panoramic camera and PTZ cameras to take the wide range images and face images at high enough resolution for identification tasks. The robust human detection methods include robust background subtraction method (RRC), skin color segmentation, and face tracking method. First, the system detects persons from a panoramic image, and then the detailed face images are obtained with the PTZ cameras. The PTZ cameras can track the faces using the four directional features and the relaxation matching. In addition, the system has an automatic camera position calibration feature. Thus, the user can use the system without any troublesome settings. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 91(11): 11–18, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecj.10006
State value compensation of controller for constrained systems
Authors: Hara Naoyuki , Kojima Akira 
Published: February 2009

AbstractState value compensation of dynamic controllers for constrained linear systems is discussed. The relation between changes in a partial state and the resulting responses is characterized by introducing a singular value decomposition (SVD). By employing the SVD, a partial state compensation law which satisfies the system constraints is derived. The advantage of the proposed method is illustrated with numerical examples. The obtained result is also applied to an inverted pendulum system for experimental evaluation. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 91(11): 1–10, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecj.10036
Visual tracking of a moving object using a stereo vision robot
Authors: Kobayashi Nobuaki , Shibata Masaaki 
Published: February 2009

AbstractIn this paper, we propose a visual tracking method for a moving object without tracking delay. On visual servoing, an image‐based method with image Jacobian is often taken for target‐centering camera motion control because it does not require the target's 3D position estimation and is robust to modeling errors and noises. In an ordinary visual servoing approach, it is assumed that the target object would be fixed in the workspace. Therefore, for the purpose of tracking to a moving target, the image errors always exist between the target position and camera optical axis, that is, the visual tracking delay. In our proposed method, the target object's 3D position is estimated with the technique based on triangulation on stereo vision system. Then, the moving velocity is calculated in the camera coordinate frame. The image Jacobian concerns the camera velocity with the target velocity in the image plane. Additionally, the target velocity in the 3D worksplace is also converted into the velocity in the image plane. The converted velocity compensation the camera velocity to compensate the camera motion delay. The approach enables us to achieve nondelayed tracking for the moving target. © 2009 Wiley Periodicals, Inc. Electron Comm Jpn, 91(11): 19–27, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecj.10184
“Disturbance observers in D‐module” for sensorless drive of synchronous motors—new phase estimation with zero phase lag
Authors: Shinnaka Shinji 
Published: February 2009

AbstractThis paper proposes two new “disturbance observers in the D‐module” for sensorless drive of synchronous motors including permanent‐magnet synchronous motors and synchronous reluctance motors. The disturbance filter for the observers can estimate speed‐varying rotor phase (in other words, rotor position) with zero phase lag. It is constructed by means of “the filter in the D‐module,” which has the following desired characteristics for variable sensorless drive: (1) it makes distinction between positive and negative frequencies of two‐phase signals; (2) it can allow processing of the signals based on frequency polarities; (3) it can also change dynamically its center frequency of bandpass filtering according to rotor speed estimates; (4) its passband and transition can be designed independently; and (5) it can be easily realized. The new disturbance observers also have a generality such that they contain the recently proposed disturbance observer as a special case. © 2009 Wiley Periodicals, Inc. Electr Eng Jpn, 167(3): 63–70, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20602
Economic evaluation of demand‐side energy storage systems by using a multi‐agent‐based electricity market
Authors: Furusawa Ken , Sugihara Hideharu , Tsuji Kiichiro 
Published: February 2009

AbstractWith the wholesale electric power market opened in April 2005, deregulation of the electric power industry in Japan has faced a new competitive environment. In the new environment, Independent Power Producer (IPP), Power Producer and Supplier (PPS), Load Service Entity (LSE), and electric utility can trade electric energy through both bilateral contracts and single‐price auction at the electricity market. In general, the market clearing price (MCP) is largely changed by the amount of total load demand in the market. The influence may cause a price spike, and consequently the volatility of MCP will make LSEs and their customers face a risk of higher revenue and cost. DSM is attractive as a means of load leveling, and has an effect on decreasing MCP at peak load period. Introducing Energy Storage systems (ES) is one DSM in order to change demand profile at the customer side. In the case that customers decrease their own demand due to increased MCP, a bidding strategy of generating companies may be changed. As a result, MCP is changed through such complex mechanism. In this paper the authors evaluate MCP by multi‐agent. It is considered that customer‐side ES has an effect on MCP fluctuation. Through numerical examples, this paper evaluates the influence on MCP by controlling customer‐side ES corresponding to variation of MCP. © 2009 Wiley Periodicals, Inc. Electr Eng Jpn, 167(3): 36–45, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20658
Structure analysis of self‐assembled ErSi2 nanowires formed on Si (110) substrates
Authors: Katayama Yusuke , Watanabe Ryouki , Kobayashi Tomohiro , Meguro Takashi , Zhao Xinwei 
Published: February 2009

AbstractThe ErSi2 nanowires were formed on Si(110) substrates by a self‐assembled growth process without a high vacuum system. All of the nanowires were highly parallel and along the Si [1‐10] direction. It was shown by structural analysis that the nanowires consisted of two types, which displayed a similar surface morphology. The first type is ErSi2 nanowires buried into the Si substrate at a depth of 30 mm, and the other is ErSi2 thin layers covering a wire‐like Si surface. The latter is suggested to be the remaining structure after evaporation of the first type wires during high‐temperature annealing. © 2009 Wiley Periodicals, Inc. Electr Eng Jpn, 167(3): 58–62, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/eej.20790
Cycloruthenated Compounds – Synthesis and Applications
Authors: Djukic Jean‐Pierre , Sortais Jean‐Baptiste , Barloy Laurent , Pfeffer Michel 
Published: February 2009

AbstractThe syntheses of cycloruthenated compounds by several methods, and especially by the C–H activation pathway, have been reviewed. Many ruthenium‐containing starting materials lead to these interesting organometallic compounds, which have found various applications in different fields of chemistry. Their reactivity highlights both thevariety of reactions that were found to occur at the Ru–C bonds as well as the inertness of the organometallic moiety when these species are exposed to strongly oxidizing molecules or to reactive halogenation reagents. Their use as catalyst precursors showed them to be particularly efficient for hydrogenation reactions, either by H2 or by hydride transfer. Physicochemical characteristics of cycloruthenated complexes include: (i) a cathodic shift of the electrochemical potentials corresponding to various redox couples, (ii) a bathochromic shift of UV/Vis absorption bands, (iii) valuable luminescence properties. Dinuclear cycloruthenated complexes display specific properties connected to interactions between the metals, such as strong electronic coupling, evidenced by intervalence bands of mixed‐valence species, or efficient energy transfer.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)