bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Bladder Tumor CellsIn vivoand in the Urine with a Peptide Identified by Phage Display
Authors: Lee Seung-Min , Lee Eun-Ju , Hong Hai-Yan , Kwon Mi-Kyung , Kwon Tae-Hwan , Choi Je-Yong , Park Rang-Woon , Kwon Tae-Gyun , Yoo Eun-Sang , Yoon Gil-Suk , Kim In-San , Ruoslahti Erkki , Lee Byung-Heon 
Published: January 2007

AbstractBladder cancer is one of the most common tumors of the genitourinary tract. Here, we use phage display to identify a peptide that targets bladder tumor cells. A phage library containing random peptides was screened for binding to cells from human bladder tumor xenografts. Phage clones were further selected for binding to a bladder tumor cell line in culture. Six clones displaying the consensus sequence CXNXDXRX/RC showed selective binding to cells from primary human bladder cancer tissue. Of these, the CSNRDARRC sequence was selected for further study as a synthetic peptide. Fluorescein-conjugated CSNRDARRC peptide selectively bound to frozen sections of human bladder tumor tissue, whereas only negligible binding to normal bladder tissue was observed. When the fluorescent peptide was introduced into the bladder lumen, in a carcinogen-induced rat tumor model, it selectively bound to tumor epithelium. Moreover, when the peptide was intravenously injected into the tail vein, it homed to the bladder tumor but was not detectable in normal bladder and control organs. Next, we examined whether the peptide can detect tumor cells in urine. The fluorescent peptide bound to cultured bladder tumor cells but not to other types of tumor cell lines. Moreover, it bound to urinary cells of patients with bladder cancer, while showing little binding to urinary cells of patients with inflammation or healthy individuals. The CSNRDARRC peptide may be useful as a targeting moiety for selective delivery of therapeutics and as a diagnostic probe for the detection of bladder cancer. (Mol Cancer Res 2007;5(1):11–19)
Stromal Cell–Derived Factor-1/Chemokine (C-X-C Motif) Ligand 12 Stimulates Human Hepatoma Cell Growth, Migration, and Invasion
Authors: Sutton Angela , Friand Veronique , Brulé-Donneger Severine , Chaigneau Thomas , Ziol Marianne , Sainte-Catherine Odile , Poiré Aurelie , Saffar Line , Kraemer Michel , Vassy Jany , Nahon Pierre , Salzmann Jean-Loup , Gattegno Liliane , Charnaux Nathalie 
Published: January 2007

AbstractIn addition to their physiologic effects in inflammation and angiogenesis, chemokines are involved in cancer pathology. The aim of this study was to determine whether the chemokine stromal cell–derived factor 1 (SDF-1) induces the growth, migration, and invasion of human hepatoma cells. We show that SDF-1 G protein–coupled receptor, chemokine (C-X-C motif) receptor 4 (CXCR4), and SDF-1 mRNA are expressed in human hepatoma Huh7 cells, which secrete and bind SDF-1. This binding depends on CXCR4 and glycosaminoglycans. SDF-1 associates with CXCR4, and syndecan-4 (SDC-4), a heparan sulfate proteoglycan at the plasma membrane of Huh7 cells, induces the growth of Huh7 cells by promoting their entry into the cell cycle, and inhibits the tumor necrosis factor-α–mediated apoptosis of the cells. SDF-1 also reorganizes Huh7 cytoskeleton and induces tyrosine phosphorylation of focal adhesion kinase. Finally, SDF-1 activates matrix metalloproteinase-9, resulting in increased migration and invasion of Huh7 cells. These biological effects of SDF-1 were strongly inhibited by the CXCR4 antagonist AMD3100, by a glycosaminoglycan, heparin, as well as by β-d-xyloside treatment of the cells, or by c-jun NH2-terminal kinase/stress-activated protein kinase inhibitor. Therefore, the CXCR4, glycosaminoglycans, and the mitogen-activated protein kinase signaling pathways are involved in these events. The fact that reducing SDC-4 expression by RNA interference decreased SDF-1–induced Huh7 hepatoma cell migration and invasion strongly indicates that SDC-4 may be an auxiliary receptor for SDF-1. Finally, the fact that CXCR4 is expressed in hepatocellular carcinoma cells from liver biopsies indicates that the in vitro results reported here could be extended to in vivo conditions. (Mol Cancer Res 2007;5(1):21–33)
A Dominant-Negative Form of the Major Human Abasic Endonuclease Enhances Cellular Sensitivity to Laboratory and Clinical DNA-Damaging Agents
Authors: McNeill Daniel R. , Wilson David M. 
Published: January 2007

AbstractApurinic/apyrimidinic (AP) endonuclease 1 (APE1) is the primary enzyme in mammals for the repair of abasic sites in DNA, as well as a variety of 3′ damages that arise upon oxidation or as products of enzymatic processing. If left unrepaired, APE1 substrates can promote mutagenic and cytotoxic outcomes. We describe herein a dominant-negative form of APE1 that lacks detectable nuclease activity and binds substrate DNA with a 13-fold higher affinity than the wild-type protein. This mutant form of APE1, termed ED, possesses two amino acid substitutions at active site residues Glu96 (changed to Gln) and Asp210 (changed to Asn). In vitro biochemical assays reveal that ED impedes wild-type APE1 AP site incision function, presumably by binding AP-DNA and blocking normal lesion processing. Moreover, tetracycline-regulated (tet-on) expression of ED in Chinese hamster ovary cells enhances the cytotoxic effects of the laboratory DNA-damaging agents, methyl methanesulfonate (MMS; 5.4-fold) and hydrogen peroxide (1.5-fold). This MMS-induced, ED-dependent cell killing coincides with a hyperaccumulation of AP sites, implying that excessive DNA damage is the cause of cell death. Because an objective of the study was to identify a protein reagent that could be used in targeted gene therapy protocols, the effects of ED on cellular sensitivity to a number of chemotherapeutic compounds was tested. We show herein that ED expression sensitizes Chinese hamster ovary cells to the killing effects of the alkylating agent 1,3-bis(2-chloroethyl)-1-nitrosourea (also known as carmustine) and the chain terminating nucleoside analogue dideoxycytidine (also known as zalcitabine), but not to the radiomimetic bleomycin, the nucleoside analogue β-d-arabinofuranosylcytosine (also known as cytarabine), the topoisomerase inhibitors camptothecin and etoposide, or the cross-linking agents mitomycin C and cisplatin. Transient expression of ED in the human cancer cell line NCI-H1299 enhanced cellular sensitivity to MMS, 1,3-bis(2-chloroethyl)-1-nitrosourea, and dideoxycytidine, demonstrating the potential usefulness of this strategy in the treatment of human tumors. (Mol Cancer Res 2007;5(1):61–70)
Differential Effects of Organic Nitrates on Endothelial Progenitor Cells Are Determined by Oxidative Stress
Authors: Thum Thomas , Fraccarollo Daniela , Thum Sabrina , Schultheiss Maximilian , Daiber Andreas , Wenzel Philip , Munzel Thomas , Ertl Georg , Bauersachs Johann 
Published: January 2007

Objective—Reduced levels and impaired function of endothelial progenitor cells (EPCs) foster development and progression of atherosclerotic lesions. Endothelial nitric oxide synthase (eNOS)–derived NO regulates EPC mobilization and function. Organic nitrates release NO, and therefore may favorably affect EPC biology.Methods and Results—We compared the effects of 2 different nitrates on circulating EPC numbers and function. Treatment of rats with pentaerythritol-trinitrate (PETriN) or isosorbide dinitrate (ISDN) increased circulating EPC levels. EPC from ISDN- but not PETriN-treated animals displayed impaired migratory capacity and increased reactive oxygen species formation in EPCs. In vitro treatment with ISDN reduced migration and incorporation of human EPCs into vascular structures on matrigel, whereas PETriN improved EPC function. ISDN, but not PETriN, increased NADPH oxidase–mediated oxidative stress in cultured human EPCs. Addition of polyethylene-glycolated superoxide dismutase or diphenyliodonium normalized both ISDN-induced superoxide anion production and impaired migratory capacity of EPCs.Conclusions—Long-acting nitrates increase levels of circulating EPCs, but differ in their effects on EPC function dependent on the induction of intracellular oxidative stress. Organic nitrates that improve EPC function may confer long-term cardiovascular protection based on their beneficial effects on EPC biology.
Carbamylated Low-Density Lipoprotein Induces Monocyte Adhesion to Endothelial Cells Through Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1
Authors: Apostolov Eugene O. , Shah Sudhir V. , Ok Ercan , Basnakian Alexei G. 
Published: January 2007

Objective—Carbamylated low-density lipoprotein (LDL), the most abundant modified LDL isoform in human blood, has been recently implicated in causing the atherosclerosis-prone injuries to endothelial cells in vitro and atherosclerosis in humans. This study was aimed at testing the hypothesis that carbamylated LDL acts via inducing monocyte adhesion to endothelial cells and determining the adhesion molecules responsible for the recruitment of monocytes.Methods and Results—Exposure of human coronary artery endothelial cells with carbamylated LDL but not native LDL caused U937 monocyte adhesion and the induction of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 adhesion molecules as measured by cell enzyme-linked immunosorbent assay. Silencing of intercellular adhesion molecule-1 by siRNA or its inhibition using neutralizing antibody resulted in decreased monocyte adhesion to the endothelial cells. Similar silencing or neutralizing of vascular cell adhesion molecule-1 alone did not have an effect but was shown to contribute to intercellular adhesion molecule-1 when tested simultaneously.Conclusions—Taken together, these data provide evidence that intercellular adhesion molecule-1 in cooperation with vascular cell adhesion molecule-1 are essential for monocyte adhesion by carbamylated low-density lipoprotein-activated human vascular endothelial cells in vitro.
Infection After Acute Ischemic Stroke
Authors: Chamorro Ángel , Urra Xabier , Planas Anna M. 
Published: January 2007

Background and Purpose—Infection after experimental focal ischemia may result from brain-induced immunodepression, but it is unsettled whether a similar syndrome occurs in human stroke.Summary of Review—Many patients develop infections shortly after acute stroke regardless of optimal management. Mortality is higher in these patients and the severity of stroke is the strongest determinant of the infectious risk. However, it is controversial whether infections promote neurological worsening or alternatively represent a marker of severe disease. The brain and the immune system are functionally linked through neural and humoral pathways, and decreased immune competence with higher incidence of infections has been demonstrated in several acute neurological conditions. In experimental brain ischemia, infections are associated with the activation of the autonomous nervous system and neuroendocrine pathways, which increase the strength of anti-inflammatory signals. A strong cytokine-mediated anti-inflammatory response was recently observed in stroke patients at higher risk of infection, although infection could not demonstrate an independent association with the progression of the symptoms.Conclusions—The appearance of infection in patients with acute stroke obeys in part to immunological mechanisms triggered by acute brain injury. An excessive anti-inflammatory response is a key facilitating factor for the development of infection, and it is likely that this immunological response represents an adaptive mechanism to brain ischemia. Contrarily, it is unclear whether infection contributes independently to poor outcome in human stroke. Overall, a better understanding of the cross-talk between the brain and the immune system might lead to more effective therapies in patients with acute stroke.
Coupled Ocean–Atmosphere Response to Seasonal Modulation of Ocean Color: Impact on Interannual Climate Simulations in the Tropical Pacific
Authors: Ballabrera-Poy J. , Murtugudde R. , Zhang R-H. , Busalacchi A. J. 
Published: January 2007

Abstract
The ability to use remotely sensed ocean color data to parameterize biogenic heating in a coupled ocean–atmosphere model is investigated. The model used is a hybrid coupled model recently developed at the Earth System Science Interdisciplinary Center (ESSIC) by coupling an ocean general circulation model with a statistical atmosphere model for wind stress anomalies. The impact of the seasonal cycle of water turbidity on the annual mean, seasonal cycle, and interannual variability of the coupled system is investigated using three simulations differing in the parameterization of the vertical attenuation of downwelling solar radiation: (i) a control simulation using a constant 17-m attenuation depth, (ii) a simulation with the spatially varying annual mean of the satellite-derived attenuation depth, and (iii) a simulation accounting for the seasonal cycle of the attenuation depth. The results indicate that a more realistic attenuation of solar radiation slightly reduces the cold bias of the model. While a realistic attenuation of solar radiation hardly affects the annual mean and the seasonal cycle due to anomaly coupling, it significantly affects the interannual variability, especially when the seasonal cycle of the attenuation depth is used. The seasonal cycle of the attenuation depth interacts with the low-frequency equatorial dynamics to enhance warm and cold anomalies, which are further amplified via positive air–sea feedbacks. These results also indicate that interannual variability of the attenuation depths is required to capture the asymmetric biological feedbacks during cold and warm ENSO events.
Amazon and Orinoco River Plumes and NBC Rings: Bystanders or Participants in Hurricane Events?
Authors: Ffield Amy 
Published: January 2007

AbstractThe Amazon and Orinoco River plumes and North Brazil Current (NBC) rings are investigated during the 1 June through 30 November Atlantic hurricane season to identify their impact on upper-ocean temperatures in the region and to draw attention to their potential role in hurricane maintenance and intensification. The analysis uses ocean temperature and salinity stratification data, infrared and microwave satellite-derived sea surface temperature (SST) data, and Atlantic tropical storm and hurricane tracks data. The Amazon–Orinoco River plume spreads into the western equatorial Atlantic Ocean forming an extensive (0°–20°N, 78°–33°W) 10–60-m-thick buoyant surface layer associated with the warmest surface temperatures (up to +3°C) in the region due to the freshwater barrier layer effect. At times the warm Amazon–Orinoco River plume is bisected by cool-surface NBC rings. For the 1960 to 2000 time period, 68% of all category 5 hurricanes passed directly over the historical region of the plume, revealing that most of the most destructive hurricanes may be influenced by ocean–atmosphere interaction with the warm plume just prior to reaching the Caribbean. Statistical analyses of tropical Atlantic SSTs and tropical cyclone wind speeds reveal a significant and unique relationship between warm (cool) SSTs in the Amazon–Orinoco River plume and stronger (weaker) tropical cyclone wind speeds between 35° and 55°W. This implies that warmer (cooler) plume SSTs due to increased (decreased) river discharge may directly contribute to a more (less) vigorous hurricane season.
Early Detection of Changes in the North Atlantic Meridional Overturning Circulation: Implications for the Design of Ocean Observation Systems
Authors: Keller Klaus , Deutsch Curtis , Hall Matthew G. , Bradford David F. 
Published: January 2007

AbstractMany climate models predict that anthropogenic greenhouse gas emissions may cause a threshold response of the North Atlantic meridional overturning circulation (MOC). These model predictions are, however, uncertain. Reducing this uncertainty can have an economic value, because it would allow for the design of more efficient risk management strategies. Early information about the MOC sensitivity to anthropogenic forcing (i.e., information that arrives before the system is committed to a threshold response) could be especially valuable. Here the focus is on one particular kind of information: the detection of anthropogenic MOC changes. It is shown that an MOC observation system based on infrequent (decadal scale) hydrographic observations may well fail in the task of early MOC change detection. This is because this system observes too infrequently and the observation errors are too large. More frequent observations and reduced observation errors would result in earlier detection. It is also shown that the economic value of information associated with a confident and early prediction of an MOC threshold response could exceed the costs of typically implemented ocean observation systems by orders of magnitude. One open challenge is to identify a feasible observation system that would enable such a confident and early MOC prediction across the range of possible MOC responses.
Precipitation and Cloud Structure in Midlatitude Cyclones
Authors: Field Paul R. , Wood Robert 
Published: January 2007

AbstractComposite mean fields and probability distribution functions (PDFs) of rain rate, cloud type and cover, cloud-top temperature, surface wind velocity, and water vapor path (WVP) are constructed using satellite observations of midlatitude cyclones from four oceanic regions (i.e., the North Pacific, South Pacific, North Atlantic, and South Atlantic). Reanalysis surface pressure fields are used to ascertain the locations of the cyclone centers, onto which the satellite fields are interpolated to give a database of ∼1500 cyclones from a two-year period (2003–04). Cyclones are categorized by their strength, defined here using surface wind speed, and by their WVP, and it is found that these two measures can explain a considerable amount of the intercyclone variability of other key variables. Composite cyclones from each of the four ocean basins exhibit similar spatial structure for a given strength and WVP. A set of nine composites is constructed from the database using three strength and three WVP ranges and is used to demonstrate that the mean column relative humidity of these systems varies only slightly (0.58–0.62) for a doubling in WVP (or equivalently a 7-K rise in sea surface temperature) and a 50% increase in cyclone strength. However, cyclone-mean rain rate increases markedly with both cyclone strength and WVP, behavior that is explained with a simple warm conveyor belt model. Systemwide high cloud fraction (tops above 440 hPa) increases from 0.23 to 0.31 as cyclone strength increases by 50%, but does not vary systematically with WVP. It is suggested that the composite fields constitute useful diagnostics for evaluating the behavior of large-scale numerical models, and may provide insight into how precipitation and clouds in midlatitude cyclones respond under a changed climate.
Variability of Tropical Diurnal Sea Surface Temperature*
Authors: Clayson Carol Anne , Weitlich Derrick 
Published: January 2007

Abstract
A dataset consisting of daily diurnal warming values from 1996 through 2000 covering the global Tropics (30°N through 30°S) at 0.25° × 0.25° resolution has been created using a parameterization for the diurnal warming developed previously. The inputs to the parameterization are the peak shortwave solar radiation [determined from International Satellite Cloud Climatology Project (ISCCP) data] and daily averaged wind speed [determined from Special Sensor Microwave Imager (SSM/I) data]. Comparisons with Tropical Ocean Global Atmosphere (TOGA) Tropical Atmosphere Ocean (TAO) and Pilot Research Moored Array in the Tropical Atlantic (PIRATA) buoys show that the biases are small (mean bias is 0.0012°C; the standard deviation and correlation are 0.26°C and 0.74) and show no discernable geographic bias.
The 5-yr average shows that throughout most regions the values are small, with higher values (approaching 1°C) in the northern Indian Ocean, the western equatorial Pacific, the equatorial eastern Pacific, and several coastal regions. An EOF analysis of the variability indicates that seasonal variability is the most dominant form for each of the basins; in the Atlantic and Pacific basins it is north–south following the solar cycle. In the Indian Ocean the seasonal cycle is dominated by monsoonal variability; both the northern and southern portions of the basin have above-mean or below-mean values at the same times. Seasonal shortwave variability is responsible for the second mode in the Indian Ocean. East–west dipole weight structures appear in the spatial patterns for mode 2 in the Pacific and mode 3 for the Atlantic and Indian Oceans. These modes also display seasonally varying characteristics, with late 1997 and early 1998 being somewhat anomalous in the Pacific and Indian Oceans.
Fall Persistence Barrier of Sea Surface Temperature in the South China Sea Associated with ENSO*
Authors: Chen Jau-Ming , Li Tim , Shih Ching-Feng 
Published: January 2007

AbstractThe authors investigate persistence characteristics of sea surface temperature (SST) in the South China Sea (SCS) in association with El Niño–Southern Oscillation (ENSO). It is found that a persistence barrier exists around October and November. This fall persistence barrier (FPB) is well recognized in the developing phase of strong ENSO cases, but becomes vague in weak ENSO and normal (non-ENSO) cases. During a strong El Niño developing year, salient features of the SCS SST anomaly (SSTA) associated with the FPB include a sign reversal between summer and winter and a rapid warming during fall. One possible cause of these SST changes, as well as the occurrence of the FPB, is the development and evolution of a low-level anomalous anticyclone (LAAC). The analyses show that the LAAC emerges in the northern Indian Ocean in early northern fall, moves eastward into the SCS during fall, and eventually anchors in the Philippine Sea in northern winter. This provides a new scenario for the generation of the anomalous Philippine Sea anticyclone previously studied. Its eastward movement appears to result from an east–west asymmetry, relative to the anticyclonic circulation center, of divergent flow and associated atmospheric vertical motion/moisture fields. The eastward passage of the LAAC across the SCS warms the underlying SST first via increased absorption of solar heating in October as it suppresses convective activities in situ, and next via decreased evaporative cooling in November and December as the total wind speed is weakened by the outer flows of the eastward-displacing LAAC. As such, the SCS SST changes quickly from a cold to a warm anomaly during fall, resulting in an abrupt change in anomaly patterns and the occurrence of the FPB. Analyses also suggest that the LAAC development during fall is relatively independent from the preceding Indian summer monsoon and the longitudinal propagation features of the ENSO-related Pacific SSTA. The aforementioned ocean–atmosphere anomalies contain an opposite polarity in a strong La Niña event. The low-level circulation anomaly weakens in intensity during weak ENSO cases and simply disappears during normal cases. As a result, the SCS FPB becomes indiscernible in these cases.