bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of monoclonal antibody for assessment of estrogen and progesterone receptors in malignant effusions
Authors: Masood Shahla 
Published: February 2007

AbstractAn immunocytochemical assay utilizing specific monoclonal antibodies against estrogen receptor (ER) and progesterone receptor (PgR) has been shown to be highly reliable for the detection of hormone receptors in hormone sensitive tumors. To assess the usefulness of this technique in malignant effusions, CytospinR (Shandon, Inc., Pittsburgh, PA 15275), preparations of 41 pleural and ascitic fluid were studied. The findings from the malignant cells employing estrogen and progesterone receptor immunocytochemical assay were compared with the results obtained from primary tumors by biochemical (dextran‐coated charcoal) assay. The results agreed in 88% for ER and 83% for PgR. This study supports the potential value of cytochemical technique in detection of hormone receptors in malignant effusions. Assessment of hormone receptors in malignant effusions may be of clinical significance, particularly in situations where the hormone receptor status of the original tumor is not known. This information may also have some diagnostic and therapeutic importance in assessment of patients presenting with metastatic tumors of unknown origin. © 1992 Wiley‐Liss, Inc.
Postoperative spindle‐cell nodule of urinary bladder with unusual intracytoplasmic inclusions
Authors: Lo James W. , Fung Christopher H. K. , Yonan Theodore , Dimauro Jose 
Published: February 2007

AbstractPostoperative spindle‐cell nodule of the urinary bladder (or in the absence of previous surgical procedure, inflammatory pseudotumor) is a reactive process of unknown etiology mimicking a sarcoma. Intracytoplasmic inclusion bodies were noted inside the atypical spindle cells on Diff‐Quik stained smears in a recent case of this entity. Although fluorescence was noted in the inclusion bodies with monoclonal antibodies against the major outer membrane protein of the Chlamydia species, the exact nature of these inclusion bodies remains unknown. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration findings of the liver in a case of Q fever
Authors: Greiner Timothy C. , Mitros Frank A. , Stapleton Jack , Van Rybroek John 
Published: February 2007

AbstractThis report describes the cytologic findings in Q fever involving the liver in a 59‐yr‐old man. The fine‐needle aspiration findings are correlated with a surgical needle biopsy performed concurrently. Cytopathologists should include Q fever in their differential diagnosis of granulomatous inflammation. Ring granulomas, which can be seen in an aspiration biopsy, should suggest the diagnosis of Q fever. © 1992 Wiley‐Liss, Inc.
AgNOR counts are useful in cervical smears
Authors: Cardillo M. R. 
Published: February 2007

AbstractBecause they present marked cellular changes, the cytological appearances of epithelial repair may be confused with those of cancer. To see whether the Ag‐NOR staining technique for nucleolar organizer regions would be useful to distinguish benign proliferative reactions (squamous metaplasia and repair) from cervical intra‐epithelial neoplasia (CIN I, CIN II, and CIN III), we studied a series of cervical smears. The smears, previously stained with the Papanicolaou technique, were destained and restained with Ag‐NOR silver. Significantly different (P < 0.05) Ag‐NOR counts were found in squamous metaplasia, epithelial repair, and various degrees of CIN. The Ag‐NOR technique appears useful in the diagnosis of cervical cytology and particularly in cases with marked cellular change, which could be confused with neoplasia. © 1992 Wiley‐Liss, inc.
Identification of acanthamoeba in bronchoalveolar lavage specimens
Authors: Newsome Anthony L. , Curtis Faune T. , Culbertson Clyde G. , Allen Stephen D. 
Published: February 2007

AbstractAcanthamoeba species infect humans occasionally and act as opportunistic pathogens in immunocompromised individuals. This study demonstrates the application of cytocentrifugation as an aid to identification of Acanthamoebae. In addition, certain staining procedures clearly optimized visualization of characteristic amoebic features. This was demonstrated by adding amoebae from laboratory cultures to bronchoalveolar lavage specimens. In preparations stained by the Papanicolaou, trichrome, or hematoxylin‐eosin (H&E) procedures, the discrete deeper staining nucleolus was the most distinctive feature. The vacuolated cytoplasm also aided in the identification of amoebae. These features were less apparent and often distorted following staining of Acanthamoeba species with the Hema III and Giemsa procedures. © 1992 Wiley‐Liss, inc.
Asteroid bodies and calcium oxalate crystals: Two infrequent findings in fine‐needle aspirates of parotid sarcoidosis
Authors: Pérez‐Guillermo M. , Sola Pérez J. , Espinosa Parra F. J. 
Published: February 2007

AbstractWe have studied 3 cases of sarcoidosis involving the parotid gland by means of fine‐needle aspiration cytology (FNAC). The main findings were noncaseating granulomas, multinucleated giant cells (MGCs), and lymphocytes. In one case MGCs contained asteroid bodies and in another case we observed calcium oxalate crystals (COCs) over both stromal fragments and MGCs. Although nonpathognomonic for sarcoidosis, these 2 findings may help in the diagnosis of this condition. However, both are easily overlooked and must be borne in mind when viewing noncaseating granulomas. Sarcoid granulomas displaying COC must be differentiated from foreign‐body granulomas. The aforementioned cytological findings must be assessed in conjunction with clinical findings. Nevertheless, in most cases the diagnosis of sarcoidosis is made by exclusion. © 1992 Wiley‐Liss, Inc.
Aspiration biopsy of granulosa‐cell tumor of the ovary: Cytologic findings and differential diagnosis
Authors: Nguyen Gia‐Khanh , Redburn Jim 
Published: February 2007

AbstractFine‐needle aspirates from four histologically confirmed adult granulosa‐cell tumors of the ovary revealed irregular sheets, loose aggregates or tight clusters of small tumor cells with round or oval nuclei and scant cytoplasm. Nuclear grooves and indentations were noted in a small number of cells in two cases. Tumor cells arranged in follicular pattern mimicking Call‐Exner bodies were not identified in all cases. A cytologic differential diagnosis of “small‐cell” tumors of the ovary is briefly discussed.
Scanning electron microscopy of body fluids
Authors: Beals Ted F. 
Published: February 2007

AbstractScanning Electron Microscopic (SEM) examination of body fluid specimens submitted for cytopathological diagnosis is technically easy and can be accomplished within hours. Specimens refrigerated for as long as 72 hr show no significant structural alterations that would interfere with recognition of diagnostically significant cells. SEM is particularly well suited for these specimens because the cells are free floating, and even cells in clusters have “natural” surfaces. Clinical uses of SEM on body fluids include the identification of ambiguous cells, distinction of reactive (benign) mesothelial cells from metastatic adenocarcinoma, and distinction of lymphoid cells from small cell carcinoma of the lung. In addition, SEM of these fluids assists cytologists to better understand the cellular features and associations seen in the light microscope. Ultrastructural analysis can be an important component of the Quality Assurance Plan for those cytodiagnostic laboratories that have access to an electron microscopy facility. © 1992 Wiley‐Liss, inc.
Electron microscopy of fine‐needle aspiration biopsies of the mediastinum
Authors: Neill James S. A. , Silverman Jan F. 
Published: February 2007

AbstractTwenty cases of fine‐needle aspiration (FNA) biopsy of mediastinal tumors with ultrastructural studies of the aspirated material were renewed. The cases were classified according to the cytologic and ultrastructural diagnosis. A specimen insufficient for ultrastructural study was obtained in five cases (25%). Refinement of the cytologic diagnosis was made in three cases (15%) and good correlation between the initial cytologic impression and the ultrastructural studies was seen in the remaining cases (60%). Comparison of different sites of FNA biopsy revealed a higher rate of ultrastructural examination in the cases involving the mediastinum. Illustrative cases of the use of electron microscopy in FNA biopsies of mediastinal tumors are presented. © 1992 Wiley‐Liss, Inc.
Electron microscopy of fine‐needle aspiration biopsy specimens: A brief review
Authors: Akhtar Mohammed , Bakry Mohammed , Al‐Jeaid Aziza Saad , McClintock Jeffrey A. 
Published: February 2007

AbstractThe cellular sample obtained by fine‐needle aspiration biopsy is usually small and therefore requires extreme care during processing for electron microscopy. The most significant technical problem is due to contamination of the sample by red blood cells, which tend to dilute the samples. Red blood cells in these samples may be removed prior to processing by either using Bovine serum albumin as a gradient or by filtration of the specimen by a nylon sieve. Experience at our institution with the use of electron microscopy for interpretation of fine‐needle aspiration biopsy samples is briefly reviewed. © 1992 Wiley‐Liss, Inc.
Electron microscopy of fine‐needle aspiration biopsy from extragonadal germ cell tumors
Authors: Dell'Antonio G. , Taccagni G. L. , Terreni M. R. , Leone B. E. , Cantaboni A. 
Published: February 2007

AbstractWe describe five cases of extragonadal germ cell tumor (EGCT) diagnosed by the electron microscope (EM) on cytological material. The clinical diagnosis was incorrect in all cases and EGCT was suspected in two cases; cytological diagnosis by light microscopy confirmed the presence of malignant tumor cells, but did not identify the cytotype/s correctly except in one case. Ultrasonography, laparoscopy. and autopsy (in case 3) excluded a primitive germ cell tumor (GCT). Histology confirmed the EM diagnosis in all cases.EM, even of scanty or necrotic cytological material, is particularly useful for mediastinal and retroperitoneal masses. In case of EGCT, EM can identify the different cytotypes and the different ultrastructural subcellular cytotypes and demonstrates a close relation between seminomatous and nonseminomatous GCT, which could influence their classification and prognosis. © 1992 Wiley‐Liss, Inc.
Rapid ultrastructural examination of FNAs in the diagnosis of intrathoracic tumors
Authors: Kurtz Stanley M. 
Published: February 2007

AbstractBy coupling radiologic imaging procedures with a fine‐needle aspiration (FNA) technique, superficial and deep‐seated tumors of the thorax may be sampled for cytopathologic evaluation with relative ease, minimal trauma to the patient, and may avert unnecessary or contraindicated surgery. However, the cytodiagnostic limitations that apply to FNA specimens from other sites also obtain for tumors of the thoracic cavity. Although application of histo‐ and immunohistochemistry offers some expansion of cytodiagnostic limits, their inherent problems of technical failure and biological capriciousness demand a highly critical if not outright skeptical appraisal of the result when applied to cytology specimens. By extending the basic morphologic principles of cytodiagnosis to include fine structure, its limitations can be largely eliminated. Journals of pathology and specialized atlases contain detailed fine structural descriptions of essentially all recognized neoplasms, and the features that discriminate one neoplasm from another are now well recognized. In most instances, a few well‐preserved cells or cell groups obtained from an FNA can provide a morphological diagnosis that rivals or surpasses that of conventional paraffin sections of surgically biopsied material, when evaluated in concert with the cytology preparation. © 1992 Wiley‐Liss, Inc.
Merits of fine‐needle aspiration biopsy in children: Head and neck
Authors: Wakely Paul E. 
Published: February 2007

Abstract(Editor's Note: Recently, I a fine‐needle aspiration biopsy specimen taken from an 18 month‐old baby. The interpretation was relatively easy because the patient had a previous diagnosis of neuroblastoma. However, I found very little information regarding the value of FNA in the pediatric age group. Can you shed light on this subject?).
Diagnostic accuracy of fine‐needle aspiration cytology in persistent or recurrent gynecologic malignancies
Authors: Wojcik Eva M. , Selvaggi Suzanne M. 
Published: February 2007

AbstractA retrospective 7 1/4‐yr study was performed to evaluate the diagnostic accuracy of fine‐needle aspiration (FNA) cytology in the cell typing of persistent or recurrent gynecologic malignancies. A total of 202 aspirates were obtained from 163 patients with documented malignancies of the cervix, uterus, ovary, vulva, and vagina. Information concerning the primary tumor was obtained from surgical reports and/or medical records. In 168/202 cases (83%), the histological diagnosis, including primary tumor cell type and subtype (ex. squamous cell carcinoma, large cell keratinizing), were available. In 12/202 cases (6%), only the tumor cell type (ex. squamous cell carcinoma) was known, and in the remaining 22 cases (11%), only the location of the primary neoplasm was attainable. Aspirated sites included pelvic wall and organs (77 cases), lymph nodes (51 cases), thoracic organs (18 cases), and abdominal wall and organs (56 cases), including liver (33 cases). Of the 168 cases with known histologic diagnoses, the FNA results were positive in 109 (65%). The positive results were divided into three groups: group I, the cytologic findings were predictive of the histologic diagnoses (84 cases, 77%); group II, tumor cell subtyping was not possible on cytology (17 cases, 16%); group III, neither tumor cell typing nor subtyping was possible on cytology (8 cases, 7%). Of the 34 cases in which only the histologic tumor cell type or primary tumor location was known, 13 (38%) were positive on FNA. Of these, the cytologic diagnoses were specific in eight cases (62%) (group I); 4 FNA results (31%) were categorized as group II and 1 case (7%) as group III. The results of this study indicate that a precise cytologic diagnosis (75% in this series; 92/122 positive cases) as to tumor cell type and subtype can be make in the majority of FNAs obtained from patients with persistent or recurrent gynecologic malignancies.
Needle aspiration cytology of the irradiated breast
Authors: Filomena Carol Ann , Jordan Andrea G. , Ehya Hormoz 
Published: February 2007

AbstractDuring a 3‐year period (1987‐1989), 60 fine‐needle aspiration biopsies (FNAs) were obtained from new breast lesions in patients previously treated by radiation and surgery for breast carcinoma. The lesions occurred at or near the site of previous excision, 3‐117 months after initiation of radiotherapy. FNAs were classified as follows: acellular (11); negative (29); atypical (13); suspicious (4); and positive (3). For statistical analysis, acellular, negative, and atypical diagnoses were considered negative findings, and suspicious and positive diagnoses were considered positive findings. On the basis of subsequent biopsy and/or patient follow‐up, FNA yielded a sensitivity of 86%, a specificity of 98%, a positive predictive value of 86%, a negative predictive value of 98%, and an efficiency of 97%. Excluding cystic lesions, the most reliable criterion for distinguishing malignant from benign lesions was the abundance of epithelial cells, both singly and in large clusters. Cellular characteristics were less helpful, since nuclear atypia was seen in both benign and malignant lesions. It is concluded that (1) FNA is a reliable technique in the evaluation of the irradiated breast; (2) when performed by an experienced operator, an acellular aspirate may be interpreted as evidence against recurrent carcinoma; and (3) epithelial atypia must be interpreted with caution to avoid a false‐positive diagnosis.
Columnar cells in posthysterectomy vaginal smears
Authors: Bewtra Chhanda 
Published: February 2007

AbstractColumnar cells in posthysterectomy vaginal smears are unusual and rare. Nine such cases are reported here during a 6‐yr period. All nine patients were asymptomatic and total hysterectomy with bilateral salpingoophorectomy for gynecological malignancies was performed 8 mo to 25 yr ago. Three patients had local radiation and one had systemic chemotherapy not less than 8 mo before the cytology. The columnar cells showed one of three patterns: 1) long, bipolar cells in sheets, resembling reparative columnar cells (4 cases), 2) goblet‐type cells with eccentric nuclei (3 cases), and 3) tight clusters of small round cells (2 cases). No consistent relationship with age, treatment, or background was seen with any of the patterns. Follow‐up of all cases by thorough pelvic examination, repeat smears, colposcopy, and biopsy showed no vaginal pathology. Benign mucinous or goblet cell metaplasia in atrophic vaginal epithelium may be the source of some of these cells in the vaginal smears.
Nucleolar organizer regions in FNAC smears of breast lesions
Authors: Misra Kiran , Kumar Neelam Dhingra 
Published: February 2007

AbstractThe argyrophil technique for staining proteins associated with nucleolar organizing regions was applied to the fine‐needle aspiration cytology smears of 45 cases of breast disease. These included 14 malignant and 31 benign lesions. A correlation with histology sections was done in 12 cases. There was a significant difference between AgNOR count of benign and malignant breast disease with one case out of 45 falling in the “gray” zone of overlap. Infiltrating lobular carcinoma was found to have the lowest NOR counts among the malignant lesions. The smear staining was superior to that on sections, the positive features being lack of background staining and better dispersion of NORs in the nucleoli.
Fine‐needle aspiration of cystic nephroma (multilocular cyst of the kidney)
Authors: Clark S. P. , Kung I. T. M. , Tang S. K. 
Published: February 2007

AbstractCystic nephromas are rare tumors of the kidney most commonly affecting boys or adult females. The fine‐needle aspiration cytomorphology has not yet been described.A renal cystic mass in a 56 year old female was aspirated under ultrasound guidance. Papanicolaou stained smears of the cyst fluid revealed markedly atypical cells forming papillary clusters. Subsequent nephrectomy showed a typical cystic nephroma with lining epithelium resembling that seen in the aspirate.The cytomorphology of cystic nephroma has been misdiagnosed as renal cell carcinoma in the literature. Low cellularity, absence of necrosis, and paucity of single cells are features that should raise the possibility of cystic nephroma in a cystic renal mass. © 1992 Wiley‐Liss, Inc.
Detection of cytomegalovirus DNA in pulmonary specimens: Confirmation by in situ hybridization in two cases
Authors: Iwa Nobuzo , Sasaki Masaomi , Yutani Chikao , Wakasa Kenichi 
Published: February 2007

AbstractPapanicolaou stained bronchial brush and imprint pulmonary smears containing intranuclear and cytoplasmic inclusion bearing alveolar pneumocytes suggestive of cytomegalovirus infection were destained and reprocessed for in situ hybridization using a biotinylated probe for cytomegalovirus DNA. Two cases were processed in this way. A hybridization signal for viral DNA was noted in each case. However, no reddish brown staining reaction products were noted in any of the control samples. This simple and rapid nonradioactive detection system is a valuable supplement to routine pulmonary cytology for the definitive diagnosis of this virus infection, and this technique is also appropriate for retrospective study. © 1992 Wiley‐Liss, Inc.
Cytologic diagnosis of malignant mesothelioma in serous effusions using an antimesothelial‐cell antibody
Authors: Donna Adalberto , Betta Pier‐Giacomo , Bellingeri Domenico , Tallarida Francesca , Pavesi Mansueto , Pastormerlo Massimo 
Published: February 2007

AbstractDifferentiating mesothelioma, reactive mesothelium, and adenocarcinoma in serous effusions is often difficult, despite the application of ancillary techniques in support of the traditional cytomorphologic criteria. A polyclonal antimesothelial‐cell antibody recently developed by our group was evaluated as a histogenetic marker on a series of primary (n = 12) and metastatic (n=12) malignant effusions. Immunostaining was performed on paraffin sections from cell blocks. All mesothelioma effusions stained positive for the antibody, whereas, in contrast, all metastatic carcinoma specimens failed to react. These results (100 percent specificity and 100% sensitivity for mesothelioma) provide a basis for a reliable use of the antibody in the cytologic examination of suspicious or malignant serous effusions.
Cytologic characteristics of congenital mesoblastic nephroma in fine‐needle aspiration cytology: A case report
Authors: Drut Ricardo 
Published: February 2007

AbstractThe cytologic features of congenital mesoblastic nephroma (CMN) as recognized in smears of fine‐needle aspirated cytology (FNAC) are reported. These included spindle‐ and tadpole‐shaped cells with round to oval nuclei having small nucleoli and a smooth contour. The cytoplasm of these cells was dense and homogeneously stained. The background was composed of mucoid fibrillar material. The findings appear to be different from other types of renal tumors in infancy and specific enough for this tumor to allow diagnosis by FNAC. © 1992 Wiley‐Liss, Inc.
Simultaneous fine‐needle aspiration of thyroid lesions and regional cervical lymph nodes: Clinicopathologic implications
Authors: Guarda Luis A. 
Published: February 2007

AbstractOccasionally patients with thyroid nodules to be evaluated by fine‐needle aspiration (FNA) biopsy also present with regional cervical lymphadenopathy. Aspiration biopsies should be performed on both the thyroid and nodal lesions. This approach will yield important information about whether the lesions are related, with profound impact on further workup, management, prognosis, and staging. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of hepatic angiosarcoma: Report of a case with immunocytochemical findings
Authors: Wong John W. , Bedard Yvan C. 
Published: February 2007

AbstractHepatic angiosarcoma (Kupffer cell sarcoma) is a very rare but ominous malignancy. We report a case diagnosed by fine‐needle aspiration biopsy (FNAB). The smear showed malignant spindle cells and a few rounded cells. The diagnosis was made on the cell block by the characteristic scaffolding arrangement of malignant cells along preexisting hepatocytes. This is the first report with immunocytochemical findings. The tumour cells stained positively for vimentin and negatively for keratin, factor VIII, Ulex europaeus agglutinin I (UEA‐1), carcinoembryonic antigen (CEA), alpha‐fetoprotein (AFP), and lysozyme. This case demonstrates the possibility of a definitive diagnosis by FNAB prior to death without inflicting serious complications.
Benign metastasizing pleomorphic adenoma of salivary gland: Diagnosis of bone lesions by fine‐needle aspiration biopsy
Authors: Pitman Martha Bishop , Thor Ann D. , Goodman Max L. , Rosenberg Andrew E. 
Published: February 2007

AbstractTwo cases of benign salivary gland pleomorphic adenomas metastatic to bone (benign‐metastasizing pleomorphic adenomas) diagnosed by fine‐needle aspiration biopsy are presented. Both primary tumors were slightly atypical cytologically but neither case demonstrated features of carcinoma. The metastatic lesions contained benign epithelial, myoepithelial, and stromal components. In both cases the clinical history was either not known by the radiologist or not communicated to the cytopathologist interpreting the case, and a primary tumor of bone was the leading clinical diagnosis. Obtaining pertinent clinical history and comparing the cytomorphology of the bone aspirate with the primary parotid tumor allowed for an accurate diagnosis in both cases. The differential diagnosis with primary bone tumors is discussed and the importance of clinical history is emphasized.