bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cortical microtubule dynamics during reaction wood formation ensures context-appropriate cellulose microfibril angle in woody trees
Authors: Tobias Larissa Machado , McFarlane Heather E. , Bossinger Gerd , Spokevicius Antanas V. 
Published: March 2024

Abstract
Key message
Cortical microtubule arrays are the primary mechanism for guiding the re-orientation of cellulose microfibrils and determining MFA in secondary cell wall of wood fibre and tracheid cells in reaction wood.

Abstract
Microtubules are directly and indirectly involved in guiding cellulose synthase complexes (CSCs) through the plasma membrane. The angle of cellulose deposition is a critical response to environmental signals and/or stress conditions, and particularly crucial during reaction wood formation, a process in which woody plants deposit additional cell wall material to counteract gravitational forces. Tubulin genes are upregulated in response to gravitational stimulus during reaction wood formation, which can result in changes to microtubule assembly. In this study, microtubules were visualised in three woody tree species (two angiosperms: Eucalyptus globulus Labill., Populus alba L., and one gymnosperm: Pinus radiata D. Don.) using immunofluorescence to quantitatively evaluate microtubule organisation during reaction wood formation. Our results suggest that reorientation of the cortical microtubule array affects secondary cell wall deposition, even across different types of reaction wood, by ensuring context-appropriate orientation of cellulose microfibrils and determining MFA in wood cells. Pharmacological studies conducted on in vitro cultured stem segments or in vivo during reaction wood formation corroborated these important roles for microtubules during wood development. This study starts to unveil the role of tubulins during wood formation by exploring cortical microtubule array organisation in trees subjected to gravitational stimulus and it sheds light on cellular and molecular mechanisms behind cellulose deposition in tree species.
Effects of root-colonizing fungi on pioneer Pinus thunbergii seedlings in primary successional volcanic mudflow on Kuchinoerabu Island, Japan
Authors: Ishikawa Akira , Hayasaka Daisuke , Nara Kazuhide 
Published: March 2024

AbstractRoot-colonizing fungi, such as mycorrhizal fungi and dark septate endophyte fungi, are often found on pioneer plant species during early primary succession. However, little is known about which fungal species are responsible for the establishment of pioneer plants when these symbionts colonize simultaneously. We investigated the root-colonizing fungal communities of Pinus thunbergii that established prior to lichens, bryophytes, and short-lived herbaceous plants in a primary successional volcanic mudflow site on Kuchinoerabu Island, Japan. We collected a total of 54 current-year and 1- to 2-year-old seedlings. The colonization of root fungi was evaluated by direct observation of key structures (e.g., mantle, arbuscule, microsclerotia, and hyphae) and molecular analysis. Of the 34 current-year seedlings collected, only 12 individuals were colonized by ectomycorrhizal (ECM) fungi. By contrast, all 1- to 2-year-old seedlings were colonized by ECM fungi. Seedlings colonized by pine-specific ECM fungi, specifically Rhizopogon roseolus and Suillus granulatus, showed higher nitrogen and phosphorus contents in their needles compared to non-ECM seedlings. Arbuscular mycorrhizal fungi and dark septate endophyte fungi were found in only two and three individuals, respectively. The high density of mycophagous deer on Kuchinoerabu-jima may contribute to the favored dispersal of ECM fungi over other root-colonizing fungi. In conclusion, the seedling establishment of P. thunbergii at the volcanic mudflow may be largely supported by ECM fungi, with negligible effects of arbuscular mycorrhizal fungi and dark septate endophytes.
The role of the Flb protein family in the life cycle of Aspergillus niger
Authors: Chen Xiaoyi , Moran Torres Juan P. , Wösten Han A. B. 
Published: March 2024

AbstractGenes flbA-E are involved in sporulation and vegetative growth in Aspergillus nidulans. Inactivation of either of these genes results in a fluffy phenotype with delayed or even abolished sporulation. Previously, a non-sporulating phenotype was obtained by inactivating flbA in Aspergillus niger, which was accompanied by lysis, thinner cell walls, and an increased secretome complexity. Here, we further studied the role of the flb genes of A. niger. Strains ΔflbA, ΔflbB and ΔflbE showed increased biomass formation, while inactivation of flbA-D reduced, or even abolished, formation of conidia. Strain ΔflbA was more sensitive to H2O2, DTT, and the cell wall integrity stress compounds SDS and Congo Red (CR). Also, ΔflbC was more sensitive to SDS, while ΔflbB, ΔflbD, and ΔflbE were more sensitive to CR. On the other hand, inactivation of flbE increased resistance to H2O2. Enzyme secretion was impacted when the Δflb strains were grown on xylose. Strain ΔflbE showed reduced xylanase, cellulase and amylase secretion. On the other hand, amylase secretion at the periphery of the ΔflbA colony was reduced but not in its center, while secretion of this enzyme was increased in the center of the ΔflbB colony but not at its periphery. Inactivation of flbC and flbD also impacted zonal cellulase and amylase activity. Together, the Flb protein family of A. niger function in biomass formation, sporulation, stress response, and protein secretion.
A self-amplifying loop of TP53INP1 and P53 drives oxidative stress-induced apoptosis of bone marrow mesenchymal stem cells
Authors: Li Fanchao , Zhang Fei , Wang Tao , Xie Zhihong , Luo Hong , Dong Wentao , Zhang Jian , Ren Chao , Peng Wuxun 
Published: March 2024

AbstractBone marrow mesenchymal stem cell (BMSC) transplantation is a promising regenerative therapy; however, the survival rate of BMSCs after transplantation is low. Oxidative stress is one of the main reasons for the high apoptosis rate of BMSCs after transplantation, so there is an urgent need to explore the mechanism of oxidative stress-induced apoptosis of BMSCs. Our previous transcriptome sequencing results suggested that the expression of P53-induced nuclear protein 1 (TP53INP1) and the tumor suppressor P53 (P53) was significantly upregulated during the process of oxidative stress-induced apoptosis of BMSCs. The present study further revealed the role and mechanism of TP53INP1 and P53 in oxidative stress-induced apoptosis in BMSCs. Overexpression of TP53INP1 induced apoptosis of BMSCs, knockdown of TP53INP1 alleviated oxidative stress apoptosis of BMSCs. Under oxidative stress conditions, P53 is regulated by TP53INP1, while P53 can positively regulate the expression of TP53INP1, so the two form a positive feedback loop. To clarify the mechanism of feedback loop formation. We found that TP53INP1 inhibited the ubiquitination and degradation of P53 by increasing the phosphorylation level of P53, leading to the accumulation of P53 protein. P53 can act on the promoter of the TP53INP1 gene and increase the expression of TP53INP1 through transcriptional activation. This is the first report on a positive feedback loop formed by TP53INP1 and P53 under oxidative stress. The present study clarified the formation mechanism of the positive feedback loop. The TP53INP1–P53 positive feedback loop may serve as a potential target for inhibiting oxidative stress-induced apoptosis in BMSCs.
Unchanged water stress induces growth retardation, histopathological alterations, and antioxidant-immune disruptions in Oreochromis niloticus: the promising role of dietary organic acids
Authors: Abdel-Aziz Mohamed F. A. , El Basuini Mohammed F. , Sadek Mohamed F. , Elokaby Mohamed A. , El-Dakar Ashraf Y. , Metwally Mohamed M. M. , Shehab Ahmed , Mabrok Mahmoud , Abdel Rahman Afaf N. 
Published: March 2024

AbstractLegal restrictions and consumer trends are contributing to the growing movement for fish welfare. Therefore, the aquaculture sector is a means of reducing animal stress and enhancing physiological state, which raises financial profits. Feed additives have therefore been suggested as possible dietary stress alleviation. In this trend, a 75-day feeding trial was conducted to evaluate the effects of three organic acids (formic acid (FA), lactic acid (LA), and commercial organic acids mix (COM)) as dietary additives in Nile tilapia (Oreochromis niloticus) reared under a stressful condition (unchanged water). Fish (weighing 3.95 ± 0.05 g) were randomly housed into eight groups (n = 210 fish/group; 70 fish/replicate; 3 replicates/group). The first (negative control) and second (positive control) groups were fed a basal diet without additives and with water exchange at 20% of the water volume every 2 days for the first group and without water exchange for the second. The other six groups (FA1, FA2, LA1, LA2, COM1, and COM2) were fed basal diets supplemented with OAs at two doses (1 and 2%) without water exchange during the trial. The results revealed that fish kept without water exchange (positive control) had the highest level of water-unionized ammonia and nitrite and a marked decline in growth performance (weight gain and specific growth rate). The unchanged water induced substantial decreases in the hematological profile (white and red blood cell count, hemoglobin value, and hematocrit %), protein profile indices (total protein, albumin, and globulin), and antioxidant-immune response (superoxide dismutase, catalase, lysozyme, and total immunoglobulin M). In addition, the biochemical indices (glucose, creatinine, urea, alanine aminotransferase, and aspartate aminotransferase) and lipid peroxide (malondialdehyde) were substantially increased in the positive control group. In addition to that, marked and severe histopathological alteration in the gills, liver, and intestine including hyperplasia, hemorrhage, and degenerative and necrotic changes that were associated with desquamation and sloughing were obvious in the PC group. Dietary LA1 significantly enhanced (P < 0.05) the overall measured indices; meanwhile, the dietary FA and COM recorded the worst results. Overall, our findings demonstrate that the unchanged water negatively affected the growth and physiological functions of Nile tilapia. Dietary incorporation of organic acids did not protect the fish from these hazards except the LA (1%). The latter can reduce nitrogenous compounds, enhance immune response, and alleviate these alterations for the sustainable aquaculture industry.
Composition as Identity and the Logical Roots of Leibniz’s Nominalism
Authors: Costantini Filippo 
Published: March 2024

AbstractThe paper deals with Leibniz’s ontology and the metaphysics of the aggregate. Concerning the ontology of aggregates, the main aim is to provide a new argument in favor of the claim that an aggregate and its constituents have the same ontological import. This argument takes the form of a weakening of a principle known in the contemporary literature of mereology as ‘composition as identity’ (CAI). The paper shows that Leibniz’s nominalism toward aggregates is a direct consequence of two elements: the way in which he considers the relationship between aggregates and their constituents in his logical calculus; and his theory of identity (and more generally, equivalence relations) as providing us with the ground for substitution salva veritate. It is concluded that Leibniz is committed to a principle that the author dubs Ontological-CAI: the aggregate/whole is ontologically identical (i.e. it has the same ontological import) as its constituents/parts. Concerning the metaphysics of aggregates, the paper outlines in what sense aggregates are grounded on their constituents: arguing that Leibniz is committed to a further principle that the author calls Metaphysical-CAI: the aggregate/whole is metaphysically grounded on its constituents/parts. From this it can be understood in which sense Leibniz could be considered a mereological nihilist, and in which sense not. The paper also sets out two different and competing readings of Metaphysical-CAI, and argues that Leibniz accepted both of them by interpreting them as different levels of explanation of the nature of aggregates.
Domain Restrictions in the Aggregation of Classifications
Authors: Craven John 
Published: March 2024

AbstractThe possibility of domain restrictions that allow the consistent use of majority-based aggregators for rankings of objects has been widely explored. This paper extends this exploration to structures in which equivalence relations or classifications are aggregated, and shows that there is very limited scope for such restrictions in the binary structure of Mirkin and in the unary structure of Maniquet and Mongin. We develop a hybrid structure that combines binary and unary conditions on the aggregator, and that allows the use of a majority-based aggregator if and only if each object is eligible for inclusion in no more than two categories out of some greater number. We also show that in many circumstances, the surjectivity requirement of Maniquet and Mongin implicitly introduces binary conditions on the aggregator, and that their structure is entailed by the hybrid structure introduced here.
Should Type Theory Replace Set Theory as the Foundation of Mathematics?
Authors: Altenkirch Thorsten 
Published: March 2024

AbstractMathematicians often consider Zermelo-Fraenkel Set Theory with Choice (ZFC) as the only foundation of Mathematics, and frequently don’t actually want to think much about foundations. We argue here that modern Type Theory, i.e. Homotopy Type Theory (HoTT), is a preferable and should be considered as an alternative.
Davidsonian Metasemantics and Radical Interpretation
Authors: Tarnowski Maciej 
Published: March 2024

AbstractIn the current debate on the metaphysical grounding of semantic properties Donald Davidson is usually taken to represent interpretationism, a stance according to which the meaning of expressions is metaphysically grounded by the process of assigning them semantic values which maximize certain parameters such as truth or rationality of the speaker. This stance is often contrasted with productivism, which takes circumstances of expression’s production, not interpretation, to ground its meaning. In this article, I argue that this widespread understanding of Davidson’s thought is false and that given such demarcation Davidson should be regarded as a productivist. To support my claim, I begin by briefly outlining the state of debate on metasemantics and main theses of Davidson’s program, and then I present three exegetical arguments against interpretationist reading of Davidson’s work. I conclude by reconstructing what I take to be the actual metaphysical picture of semantic properties emerging from Davidson’s work.
Near-infrared spectroscopy and multivariate analysis as real-time monitoring strategy of TEMPO-mediated oxidation of cellulose fibers from different feedstocks
Authors: Mazega André , Fortuny Montserrat , Signori-Iamin Giovana , Aguado Roberto J. , Tarrés Quim , Santos Alexandre F. , Delgado-Aguilar Marc 
Published: March 2024

AbstractThe regioselective oxidation of the primary hydroxyl groups of cellulose, usually mediated by the (2,2,6,6-tetramethylpiperidin-1-yl)oxyl radical (TEMPO), is highly popular in the scientific literature. However, the lack of efficient monitoring techniques imposes a severe limitation to its upscaling. This work involves a portable, user-friendly near-infrared spectroscopy device, optimized preprocessing techniques, and multivariate calibration to quickly estimate the carboxyl group content of modified cellulose (i.e., the extent of the oxidation). For that, bleached pulps from eucalyptus, pine, hemp, and sisal were submitted to TEMPO-mediated oxidation, varying the dosage of spent oxidizer (NaClO) and thus attaining samples of different values of carboxyl group content. These values were related to near-infrared spectra (908–1676 nm) by Partial Least Squares regression, yielding cross-validation coefficients (RCV2) above 0.97 for wood pulps, 0.95 for sisal pulp, and 0.91 for hemp pulp. Based on the residual prediction deviation, the model for each pulp was found to show good predictability. Nonetheless, the overall regression model, comprising the four different materials, was unreliable. In light of this, spectra were submitted to principal components analysis (PCA), hinting that pulps could be classified in terms of their hemicellulose to cellulose ratio. Considering all the statistical parameters, the overall proposal presented here begins with a PCA—Linear Discriminant Analysis model to classify the sample by its fiber type, subsequently selecting a specific regression model for that class. Overall, the presented models in this work allow the determination of the extent of oxidation of different cellulosic feedstocks, expressed as carboxyl content, in a fast and simple approach using a benchtop near-infrared equipment.
Debt Stabilisation and Dynamic Interaction Between Monetary Authority and National Fiscal Authorities
Authors: Gori Luca , Purificato Francesco , Sodini Mauro 
Published: March 2024

Abstract
The main aim of the present research is to consider a monetary union’s economy consisting of N countries, N fiscal authorities (one for each country) and a single monetary authority. The fiscal authorities want to stabilise output and public debt through the primary government balance, and they can exhibit heterogeneous preferences about the trade-off between output and debt stability. Unlike these, the monetary authority has the aim of price and output stability. They play a non-cooperative policy game, in which they independently and simultaneously choose monetary and fiscal instruments to pursue their goals. In a dynamic setting, each authority must choose its policy instrument prevailing in the next period without knowing—at the end of each period—the choice of other authorities. By assuming static expectations, the present work shows the possibility of several dynamic outcomes. First, there exists one Nash equilibrium representing the optimal level for the macro economy; this equilibrium is stable if the average weight that fiscal authorities assign to output stability is not excessively high; therefore, this result holds even if some authorities are less willing to promote debt stabilisation. Second, in addition to this equilibrium, there exist other Nash equilibria representing steady-state values for macroeconomic variables that differ from the targets adopted by the authorities; these equilibria emerge and are stable if the authorities’ preference for output stability is even greater and with a higher degree of heterogeneity compared to the previous case. Third, the parameters of the model matter to determine the stability properties of the equilibria, and the analysis shows the possibility of nonlinear dynamics.
Functional principal component analysis for incomplete space–time data
Authors: Palummo Alessandro , Arnone Eleonora , Formaggia Luca , Sangalli Laura M. 
Published: March 2024

AbstractEnvironmental signals, acquired, e.g., by remote sensing, often present large gaps of missing observations in space and time. In this work, we present an innovative approach to identify the main variability patterns, in space–time data, when data may be affected by complex missing data structures. We formalize the problem in the framework of functional data analysis, proposing an innovative method of functional principal component analysis (fPCA) for incomplete space–time data. The functional nature of the proposed method permits to borrow information from measurements observed at nearby spatio-temporal locations. The resulting functional principal components are smooth fields over the considered spatio-temporal domain, and can lead to interesting insights in the spatio-temporal dynamic of the phenomenon under study. Moreover, they can be used to provide a reconstruction of the missing entries, also under severe missing data patterns. The proposed model combines a weighted rank-one approximation of the data matrix with a roughness penalty. We show that the estimation problem can be solved using a majorize–minimization approach, and provide a numerically efficient algorithm for its solution. Thanks to a discretization based on finite elements in space and B-splines in time, the proposed method can handle multidimensional spatial domains with complex shapes, such as water bodies with complicated shorelines, or curved spatial regions with complex orography. As shown by simulation studies, the proposed space–time fPCA is superior to alternative techniques for Principal Component Analysis with missing data. We further highlight the potentiality of the proposed method for environmental problems, by applying space–time fPCA to the study of the lake water surface temperature (LWST) of Lake Victoria, in Central Africa, starting from satellite measurements with large gaps. LWST is considered one of the fundamental indicators of how climate change is affecting the environment, and is recognized as an essential climate variable.
Life-course social participation and physical activity in midlife: longitudinal associations in the 1970 British Cohort Study (BCS70)
Authors: Tsoli Stergiani , Fancourt Daisy , Sullivan Alice , Hamer Mark , Ploubidis George B. , Kawachi Ichiro 
Published: March 2024

AbstractA hypothesized benefit of social participation is that it encourages people to be more physically active. However, limited evidence exists on the association between social participation over the life-course and physical activity in midlife. We sought to apply a life-course framework to examine the association of social participation and device measured physical activity in midlife in the UK. We used the 1970 British Birth Cohort Study (BCS70), which includes all people born in Britain during a single week in 1970. Social participation was assessed at ages 16, 30, 34 and 42. Physical activity was measured by accelerometery at age 46, as mean daily step count and time spent in moderate to vigorous physical activity (MVPA). The associations of social participation and physical activity were tested using two different life-course models: the sensitive period model and the accumulation model. Individuals with medium and high participation compared to no social participation over their life-course had higher mean daily step count and MVPA in midlife, supporting the accumulation model. In the sensitive period model, only those that actively participated at age 42 had higher mean daily steps and MVPA compared to those who did not participate. Our study provides empirical evidence on the importance of sustaining social participation at all ages over the life-course rather than at a particular timepoint of someone’s life. If our findings reflect causal effects, interventions to promote social participation throughout the life-course could be an avenue to promote physical activity in middle life.