bluuebunny/crossref_metadata_2025_split huggingface dataset:
A petrographic study of the Rotliegendes Sandstone reservoir (Lower Permian) in the Rough Gas Field
Authors: Goodchild M. W. , Whitaker J. H. McD. 
Published: November 2006

AbstractThe diagenetic history of the Rotliegendes Sandstone reservoir in the Rough Gas Field was studied using thin-sections, XRD analyses and SEM. The Rotliegendes comprises a sequence of fine-grained fluvial sheet-flood sandstones and coarse, gravelly, low-sinuosity channel sandstones, with thin aeolian interbeds, overlain by a sequence of aeolian dune and interdune sandstones. Early, environmentally-related diagnesis (eogenesis) shows a marked variability with sedimentary facies. Within aeolian sandstones, poikilotopic anhydrite and fine, rhombic dolomite are preserved. Fluvially-derived sandstones typically contain infiltrated detrital clays and early authigenic mixed-layer clays, together with coarse, framework-displacive dolomite. Feldspars show varying degrees of alteration within all facies. These eogenetic features reflect patterns of groundwater movement during the Rotliegendes and early Zechstein. Mineral dissolution and precipitation were controlled by the chemistry of the groundwaters. Burial diagenetic (mesogenetic) features are superimposed on eogenetic cements. Authigenic clays have been converted to illitic clays. In addition, mesogenetic chlorite has formed and quartz and strongly ferroan dolomite cements are recognized. These minerals may be related to clay diagenesis within the underlying Carboniferous Coal Measures. Early, framework-supporting anyhdrite, and both phases of dolomite, have been partially dissolved, creating secondary porosity. This is attributed to the action of acidic porewaters, generated by the maturation of organic material within the Carboniferous. Post-dissolution kaolinite, gypsum and minor pyrite infill secondary pores. Gas emplacement from the Late Cretaceous onwards effectively halted further diagenetic reactions.
Diagenesis of a deeply buried sandstone reservoir: Hild Field, Northern North Sea
Authors: Lønøy A. , Akselsen J. , Rønning K. 
Published: November 2006

AbstractCalcite cementation and extensive dissolution of feldspar with formation of authigenic kaolinite, quartz cement and secondary porosity are the main diagenetic processes in the deeply buried Hild Field. Mineralogical and isotopic analyses, reservoir pressure and depositional environment suggest that these diagenetic processes occurred prior to burial at a depth of 1·5–2 km. The timing of the diagenetic sequence suggests that feldspar dissolution is related to meteoric water flow. Calcite occurs as an early diagenetic iron-poor cement, and as two types of later diagenetic (<120°C) ferroan calcite cements. The ferroan calcites are mainly an in situ dissolution-reprecipitation product of the early diagenetic phase. Extensive local dissolution of calcite was important for forming secondary porosity which is closely associated with a prominent ‘gas chimney’ in the area studied. A high CO2 content in the natural gases of the reservoir suggests that the solvent was carbonic acid formed from CO2 generated during thermal maturation of organic matter. Calcite dissolution probably occurred between 70° and 100°C.
The effect of fluid/rock ratio on feldspar dissolution and illite formation under reservoir conditions
Authors: Huang W. L. , Bishop A. M. , Brown R. W. 
Published: November 2006

AbstractExperiments on the conversion of feldspar to illite reveal that variation of the fluid/rock ratio (flow rate) has a significant effect on the kinetics of feldspar dissolution and illite formation. Dissolution of albite in near-neutral KCl solution at 200°C and 500 bar shows that the Si and Na release rate per unit surface area of albite is faster with higher fluid/rock ratio. These results have application to the interpretation of secondary porosity formation. The experiments also reveal a special set of conditions under which rapid illite formation can occur. In contrast to the slow process of illite formation in neutral solution, there can be mass nucleation and growth of illite platelets on albite surfaces in an initially acidic solution with low fluid/rock ratio. In similar experiments with higher fluid/rock ratio, kaolinite forms instead of illite because there is insufficient solid to titrate the large volume of solution. This study suggests that illitization of feldspar or kaolinite may be triggered by a decreased rate of acidic fluid-influx during burial diagenesis.
The development and destruction of porosity within Upper Jurassic reservoir sandstones of the Piper and Tartan Fields, Outer Moray Firth, North Sea
Authors: Burley S. D. 
Published: November 2006

AbstractUpper Jurassic Piper Formation sandstones of the Outer Moray Firth Basin, UK North Sea, form the main reservoir for the Piper and Tartan Fields, and are sealed by either the Kimmeridge Clay or Cretaceous marls. The main reservoir sandstones can be broadly considered as stacked coarsening-upward units that accumulated in a predominantly high-energy, shallow marine complex with depositional waters of inferred normal marine salinity, Eh and ionic composition. The amount of porosity and its distribution throughout these sandstones differs between the individual structural entities of the Piper and Tartan Fields. On the Piper structure all the sandstones are highly porous. Oil-zone porosities average 25% and are only slightly reduced in the water zone. Sandstones in the upthrown block of the Tartan structure are also generally porous but porosities are typically reduced to below 20%. Porosities are the most variable in the Tartan downthrown block and in the 15/17-9 well, off-structure Piper, where porous sandstones with between 14 and 18% porosity are associated with highly compacted and cemented sandstones with <5% porosity. Thin-section and SEM textural evidence indicate that much of the porosity in these sandstones is secondary, produced through the dissolution of an intergranular cement that provided support for the detrital framework, was peripherally replacive with respect to most detrital grains, and extensively replaced feldspars. Nodular concretions and sporadic, irregular crystals of ferroan calcite are inferred to be the remnants of this intergranular cement. The distribution of porosity zones in the Tartan downthrown block is related to the present structure and oil-water contact, supporting the interpretation of a secondary origin for much of the present porosity. Highly compacted sandstones interbedded with the porous sandstones result from a combination of early compaction, brittle framework collapse following the generation of secondary porosity and late grain-to-grain contact dissolution. Aggressive fluids responsible for the generation of the cement dissolution porosity are inferred to have been expelled from the Kimmeridge Clay in the adjacent Witch Ground Graben. The preservation of secondary porosity in Piper Formation sandstones is due to the relative timing of secondary porosity generation and subsequent hydrocarbon migration.
Interleukin-1R antagonist gene and pre-natal smoke exposure are associated with childhood asthma
Authors: Ramadas R. A. , Sadeghnejad A. , Karmaus W. , Arshad S. H. , Matthews S. , Huebner M. , Kim D-Y. , Ewart S. L. 
Published: November 2006

The interleukin-1 receptor antagonist (IL1RN) is a potent anti-inflammatory cytokine. In the present study, association of the humanIL1RNgene polymorphisms with asthma, bronchial hyperresponsiveness and forced expiratory volume in one second/forced vital capacity ratio was tested and the data was stratified by environmental tobacco smoke exposure in order to investigate a gene-smoking interaction.In an unselected subset (n = 921) of the Isle of Wight birth (UK) cohort, which has previously been evaluated for asthma and related manifestations at ages 1, 2, 4 and 10 yrs, threeIL1RNsingle nucleotide polymorphisms (SNP) were genotyped. Logistic regression and repeated measurement models for tests of association using a representative SNP rs2234678 were used, as all SNPs tested were in strong linkage disequilibrium.In the overall analysis, the SNP rs2234678 was not associated with asthma. However, in the stratum with maternal smoking during pregnancy the rs2234678 GG genotype significantly increased the relative risk of asthma in children, both in analyses of repeated asthma occurrences and persistent asthma.In conclusion, the present results show that in the first decade of life, the gene–environment interaction of the interleukin-1 receptor antagonist gene polymorphism rs2234678 and maternal smoking during pregnancy increased the risk for childhood asthma.
ACEI/D but notAGT(-6)A/G polymorphism is a risk factor for mortality in ARDS
Authors: Adamzik M. , Frey U. , Sixt S. , Knemeyer L. , Beiderlinden M. , Peters J. , Siffert W. 
Published: November 2006

The intrapulmonary renin–angiotensin systemviatissue concentration of angiotensin II or bradykinin may have multiple effects on pulmonary pathophysiology. Therefore, it was investigated whether the presence of the D allele of the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) polymorphism or the A allele of angiotensinogen (AGT) promoter polymorphism (-6)A/G are independent risk factors for 30-day survival in acute respiratory distress syndrome (ARDS) patients.In a prospective study, adults (Germans of Caucasian ethnicity)with ARDS (n = 84) were recruited from the current authors’ intensive care unit and genotyped for theACEI/D and theAGT(-6)A/G polymorphisms, as were 200 healthy Caucasian controls.Mortality was increased in theACEDD genotype compared with the I allele, and theACEI/D polymorphism was an independent prognostic factor for 30-day survival. Patients with a homozygous DD genotype were at highest risk for death (hazard ratio 5.7; 95% confidence interval 1.7–19.2) compared with the II genotype. In contrast, theAGT(-6)A/G polymorphism was neither associated with an increased risk for development of ARDS nor with outcome.In patients with acute respiratory distress syndrome, the angiotensin-converting enzyme insertion/deletion polymorphism but not the angiotensinogen (-6)A/G promoter polymorphism is an independent risk factor with a pronounced effect on 30-day survival.
Beclomethasone/formoterolversusbudesonide/formoterol combination therapy in asthma
Authors: Papi A. , Paggiaro P. L. , Nicolini G. , Vignola A. M. , Fabbri L. M. 
Published: November 2006

The present study was designed to compare the fixed combination of beclomethasone and formoterol in a hydrofluoroalkane Modulite® (Chiesi Farmaceutici, Parma, Italy) pressurised metered-dose inhaler (pMDI), with a combination of budesonide and formoterol administeredviaa Turbuhaler® (AstraZeneca, Lund, Sweden) dry powder inhaler (DPI).This was a phase III, multinational, multicentre, double-blind, double-dummy, randomised, two-arm parallel groups, controlled study design. After a 2-week run-in period, 219 patients with moderate-to-severe asthma were randomised to a 12-week treatment with beclomethasone 200 μg plus formoterol 12 μgb.i.d.deliveredviaa pMDI or budesonide 400 μg plus formoterol 12 μgb.i.d.deliveredviaa DPI.The analysis of noninferiority on primary outcome, morning peak expiratory flow in the last 2 weeks of treatment, showed no difference between groups. A statistically significant improvement from baseline in lung function, symptoms and rescue medication use was observed in both groups at all time-points. No differences were observed between treatments in either rate of asthma exacerbations or frequency of adverse events.The new fixed combination of beclomethasone and formoterol in hydrofluoroalkane Modulite® pressurised metered-dose inhaler is equivalent to the marketed combination of budesonide and formoterol in terms of efficacy and tolerability profile.
BALF-derived fibroblasts differ from biopsy-derived fibroblasts in systemic sclerosis
Authors: Scheja A. , Larsen K. , Todorova L. , Tufvesson E. , Wildt M. , Åkesson A. , Hansson L. , Ellis S. , Westergren Thorsson G. 
Published: November 2006

Growth of fibroblasts from bronchoalveolar lavage fluid (BALF) in patients with systemic sclerosis (SSc) has previously been described. The purpose of the present study was to characterise fibroblasts from BALF and bronchial biopsies from SSc patients with alveolitis and from controls, to analyse fibroblast proliferation, migration, stress fibres and proteoglycan production.BALF and bronchial biopsies were collected from 10 patients with SSc and alveolitis and from 15 controls.Outgrowth of fibroblasts was observed from the BALF of four patients, particularly in those with a markedly increased percentage of eosinophils in BALF, but not in any member of the control group. Increased levels of granulocyte-macrophage colony-stimulating factor, correlating with the percentage of eosinophils in BALF, were found in patients when compared with controls. Fibroblasts from BALF showed an elongated, mobile phenotype and increased proteoglycan production compared to the corresponding biopsy fibroblasts.In conclusion, outgrowth of fibroblasts with an altered phenotype is reported from bronchoalveolar lavage fluid in systemic sclerosis patients with alveolitis and an increased percentage of eosinophils in the bronchoalveolar lavage fluid. These findings indicate a possible role for eosinophil–fibroblast interaction in pulmonary fibrosis in systemic sclerosis.
Serendipitous identification of natural Intergenotypic recombinants of hepatitis C in Ireland
Authors: Moreau Isabelle , Hegarty Susan , Levis John , Sheehy Patrick , Crosbie Orla , Kenny-Walsh Elizabeth , Fanning Liam J 
Published: November 2006

Abstract

Background
Recombination between hepatitis C single stranded RNA viruses is a rare event. Natural viable intragenotypic and intergenotypic recombinants between 1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically recombinants represent an intriguing challenge. Hepatitis C genotype is defined by interrogation of the sequence composition of the 5' untranslated region [5'UTR]. Occasionally, ambiguous specimens require further investigation of the genome, usually by interrogation of the NS5B region. The original purpose of this study was to confirm the existence of a suspected mixed genotype infection of genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two specimens from two separate individuals. This initial identification of genotype was based on analysis of the 5'UTR of the genome by reverse line probe hybridisation [RLPH].

Results
The original diagnosis of a mixed genotype infection was not confirmed by clonal analysis of the NS5B region of the genome. The phylogenetic analysis indicated that both specimens were natural intergenotypic recombinant forms of HCV. The recombination was between genotypes 2k and 1b for both specimens. The recombination break point was identified as occurring within the NS2 region of the genome.

Conclusion
The viral recombinants identified here resemble the recombinant form originally identified in Russia. The RLPH pattern observed in this study may be a signature indicative of this particular type of intergenotype recombinant of hepatitis C meriting clonal analysis of NS2.
The homeobox transcription factor Even-skipped regulates acquisition of electrical properties in Drosophila neurons
Authors: Pym Edward CG , Southall Tony D , Mee Christopher J , Brand Andrea H , Baines Richard A 
Published: November 2006

Abstract
Background
While developmental processes such as axon pathfinding and synapse formation have been characterized in detail, comparatively less is known of the intrinsic developmental mechanisms that regulate transcription of ion channel genes in embryonic neurons. Early decisions, including motoneuron axon targeting, are orchestrated by a cohort of transcription factors that act together in a combinatorial manner. These transcription factors include Even-skipped (Eve), islet and Lim3. The perdurance of these factors in late embryonic neurons is, however, indicative that they might also regulate additional aspects of neuron development, including the acquisition of electrical properties.

Results
To test the hypothesis that a combinatorial code transcription factor is also able to influence the acquisition of electrical properties in embryonic neurons we utilized the molecular genetics of Drosophila to manipulate the expression of Eve in identified motoneurons. We show that increasing expression of this transcription factor, in two Eve-positive motoneurons (aCC and RP2), is indeed sufficient to affect the electrical properties of these neurons in early first instar larvae. Specifically, we observed a decrease in both the fast K+ conductance (IKfast) and amplitude of quantal cholinergic synaptic input. We used charybdotoxin to pharmacologically separate the individual components of IKfast to show that increased Eve specifically down regulates the Slowpoke (a BK Ca2+-gated potassium channel), but not Shal, component of this current. Identification of target genes for Eve, using DNA adenine methyltransferase identification, revealed strong binding sites in slowpoke and nAcRα-96Aa (a nicotinic acetylcholine receptor subunit). Verification using real-time PCR shows that pan-neuronal expression of eve is sufficient to repress transcripts for both slo and nAcRα-96Aa.

Conclusion
Taken together, our findings demonstrate, for the first time, that Eve is sufficient to regulate both voltage- and ligand-gated currents in motoneurons, extending its known repertoire of action beyond its already characterized role in axon guidance. Our data are also consistent with a common developmental program that utilizes a defined set of transcription factors to determine both morphological and functional neuronal properties.
High-Volume Extraction of Nucleic Acids by Magnetic Bead Technology for Ultrasensitive Detection of Bacteria in Blood Components
Authors: Störmer Melanie , Kleesiek Knut , Dreier Jens 
Published: November 2006

Abstract
Background: Nucleic acid isolation, the most technically demanding and laborious procedure performed in molecular diagnostics, harbors the potential for improvements in automation. A recent development is the use of magnetic beads covered with nucleic acid–binding matrices. We adapted this technology with a broad-range 23S rRNA real-time reverse transcription (RT)-PCR assay for fast and sensitive detection of bacterial contamination of blood products.
Methods: We investigated different protocols for an automated high-volume extraction method based on magnetic-separation technology for the extraction of bacterial nucleic acids from platelet concentrates (PCs). We added 2 model bacteria, Staphylococcus epidermidis and Escherichia coli, to a single pool of apheresis-derived, single-donor platelets and assayed the PCs by real-time RT-PCR analysis with an improved primer–probe system and locked nucleic acid technology. Coamplification of human β2-microglobulin mRNA served as an internal control (IC). We used probit analysis to calculate the minimum concentration of bacteria that would be detected with 95% confidence.
Results: For automated magnetic bead–based extraction technology with the real-time RT-PCR, the 95% detection limit was 29 × 103 colony-forming units (CFU)/L for S. epidermidis and 22 × 103 CFU/L for E. coli. No false-positive results occurred, either due to nucleic acid contamination of reagents or externally during testing of 1030 PCs.
Conclusions: High-volume nucleic acid extraction improved the detection limit of the assay. The improvement of the primer–probe system and the integration of an IC make the RT-PCR assay appropriate for bacteria screening of platelets.
Precise Spatial Relationships between Mossy Fibers and Climbing Fibers in Rat Cerebellar Cortical Zones
Authors: Pijpers Angelique , Apps Richard , Pardoe Joanne , Voogd Jan , Ruigrok Tom J. H. 
Published: November 2006

Classically, mossy fiber and climbing fiber terminals are regarded as having very different spatial distributions in the cerebellar cortex. However, previous anatomical studies have not studied these two major cerebellar inputs with sufficient resolution to confirm this assumption. Here, we examine the detailed pattern of collateralization of both types of cerebellar afferent using small injections of the bidirectional tracer cholera toxin b subunit into the posterior cerebellum. The cortical and zonal location of these injections was characterized by mapping climbing fiber field potentials, the distribution of retrogradely labeled olivary neurons, and the intrinsic zebrin pattern of Purkinje cells.Labeled climbing fiber collaterals were distributed as longitudinal strips and were always accompanied by clusters of labeled mossy fiber rosettes in the subjacent granular layer. Two- and three-dimensional reconstructions and quantitative analysis showed that mossy fibers also collateralized to other stripe-like regions usually below Purkinje cells with the same zebrin-positive or zebrin-negative characteristics as that of the injection site and associated climbing fiber collaterals. The distribution of retrogradely labeled neurons in two major sources of mossy fibers, the lateral reticular and basilar pontine nuclei, revealed interlobular and some interzonal differences.These data indicate that nonadjacent cerebellar zones, sharing the same climbing fiber input and zebrin identity, also share a common mossy fiber input. Other cerebellar cortical regions that receive collaterals from the same mossy fibers usually also have the same zebrin signature. Together with the distribution of neurons in precerebellar centers, the findings suggest a revision of the modular hypothesis for information processing in the cerebellar cortex.
Allometric Relationships between Size of Calcified Structures and Round Goby Total Length
Authors: Dietrich J. P. , Taraborelli A. C. , Morrison B. J. , Schaner T. 
Published: November 2006

Abstract
Allometric relationships between calcified structures and total length of round goby Neogobius melanostomus were determined from a sample of fish ranging in total length from 53 to 152 mm. Each of the 10 allometric equations gave coefficients of determination (r2) that were greater than 0.80 and highly significant. The equations with the most predictive ability were those relating total length to cleithral, opercular, mouth, and pharyngeal bone measurements. Round goby remains were found in the stomachs of 10 predator species from the Bay of Quinte, Lake Ontario. Round goby were ingested most often by yellow perch Perca flavescens (40% of the stomachs examined) and largemouth bass Micropterus salmoides (27%) and less frequently by other predators. Round goby calcified structures most frequently found in the stomachs of predators were mouth parts (premaxilla and dentary), pharyngeal bones (dentigerous plate and pharyngobranchial plates), and otoliths. The predictive equations, diagnostic features of calcified structures, and list of predatory species in this study may serve as helpful tools for future analyses of round goby predator diets.
Lapatinib: Current Status and Future Directions in Breast Cancer
Authors: Moy Beverly , Goss Paul E. 
Published: November 2006

AbstractLapatinib is an oral receptor tyrosine kinase inhibitor, targeting both the ErbB-1 and ErbB-2 receptors. Pre-clinical in vitro and in vivo models indicate that lapatinib is active as monotherapy, synergistically in combination with trastuzumab, and in trastuzumab-resistant cell lines. Early clinical trials also provide evidence in patients that lapatinib is active against breast cancer. This paper reviews results of phase II and III clinical trials of lapatinib in metastatic breast cancer, evidence for its potential in patients with brain metastases, and current clinical trials as adjuvant treatment in early-stage disease. Our improved understanding of the biology of breast cancer and the use of biomarkers for identification of specific subtypes is allowing us to bring patient-specific novel therapies such as lapatinib to the clinic.