bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Predictive Value of Preoperative Fasting Blood Glucose Variability for Postoperative Infection in Orthopedic Patients
Authors: Xiong Hongping , Fan Dingfang , Xu Jing , Zhou Qing , Lin Fengfei 
Published: March 2024

Abstract
Background
To explore the predictive value of preoperative fasting blood glucose variability for postoperative infections in orthopedic patients.
Methods
A retrospective study was conducted to collect the medical records of 310 patients with type 2 diabetes who were hospitalized in the orthopaedics department of Fuzhou Second General Hospital from January 2022 to November 2023 by using the hospital electronic medical record system. According to whether there was postoperative infection, they were divided into two groups: no postoperative infection group (n = 227) and postoperative infection group (n = 83). The researchers designed a basic information scale to collect general patient information and fasting blood glucose (FPG) indicators from admission to surgery. Based on at least three fasting blood glucose values, FPG variability indicators were calculated, including standard deviation of fasting blood glucose (FPG-SD), coefficient of variation of fasting blood glucose(FPG-CV), and mean true variation of fasting blood glucose (FPG-ARV). Using univariate and multivariate logistic binary regression analysis to determine the independent risk factors for postoperative infection, analyzing the correlation between FPG-SD, FPG-CV, FPG-ARV and postoperative infection through point binary correlation studies, drawing receiver operating characteristic (ROC) curves, and calculating the area under the curve (AUC) to evaluate FPG-SD, FPG-CV The value of FPG-ARV alone and in combination in predicting postoperative infections in orthopedic patients.
Results
FPG-SD, FPG-CV, and FPG-ARV were all independent risk factors for postoperative infection in orthopedic patients (P < 0.05); According to point two column correlation analysis, FPG-SD, FPG-CV, FPG-ARV are positively correlated with the occurrence of postoperative infections in orthopedic patients (r > 0, P < 0.05); Draw ROC curves, and the
Contribution of Atmospheric Circulation to Interannual Variability of Ozone Concentrations in China and Impact Mechanisms
Authors: Chen Bin , Zhao Lin , Song Zhihao , Chen Ruming , Wang Yixuan , Hu Jiashun 
Published: March 2024

Abstract
It’s essential to clarify the complex interactions between atmospheric circulation, climate, and ozone pollution. In this study, we observed that meteorological factors and circulation types affecting ozone concentrations in China exhibit significant regional differences. The interannual variability of atmospheric circulation strongly influence the interannual variability of ozone, with the contribution of it varying from 74% to 99% across the 10 regions we examined. Interannual fluctuations in ozone concentration are primarily caused by changes in the intensity of atmospheric circulation types. Additionally, among all circulation types, heavily polluted type of Chinese ozone accounts for 23.95%, caused by EOF1 mode (Arctic Oscillation), while EOF2 mode is identified as impact mechanisms for the clean type of Chinese ozone. However, circulation that leads to ozone pollution will increase, and it is noteworthy that Chinese ozone levels under the comprehensive influence of atmospheric circulation and meteorology are expected to increase in the foreseeable future.
Integrating Newborn Genetic Screening with Traditional Screening to Improve Newborn Screening
Authors: Men Shuai , Wang Zhiwei , Tang Xinxin , Liu Shuang , Liu Shuaimei , Zhao Yali , Wu Yulin , Wang Leilei 
Published: March 2024

Abstract

Background: Traditional newborn screening (NBS) for inborn errors of metabolism (IEM) and deafness has limitations due to the detection of fewer genetic disorders and variants, higher false-positive rates, and longer detection periods. This study aimed to explore the clinical validity of newborn genetic screening (NBGS) in newborns with IEM and deafness.
Methods: We retrospectively enrolled 223 cases screened for IEM by tandem mass spectrometry (MS/MS)-next-generation sequencing (NGS), including 55 positive, 68 suspected positive, and 100 negative cases. Additionally, 196 cases screened for deafness were enrolled, including 96 variant-positive and 100 negative cases. Dry blood spot samples from the newborns were used for NBGS.
Results: For IEM, NBGS detected 34 positives in 55 positive cases with a sensitivity of 61.8% (34/55), whereas variants were not detected in 21 cases. Four additional positive cases were found, including one at risk of glucose-6-phosphate dehydrogenase deficiency and three at risk of deafness. The diagnostic time observed between the two methods exhibited a significant difference: 13 days for NBGS and 35 days for MS/MS-NGS. For deafness, the consistency in the positive results between the two methods was 96.9% (93/96). Unexpectedly, three mitochondrial gene (MT-RNR1) heterogeneous variants (m.1555A>G and m.7445A>G) were not detected by NBGS. We also detected nine variants out of 100 negative cases, including seven GJB2 (c.109G>A), one GJB3 (c.547G>A), and one MYO15A (c.10250_10252delCCT), with a 9% (9/100) detection rate by NBGS.
Conclusion: As a novel screening method for newborns, NBGS can detect more gene variants, reduce the false-positive rate, and shorten the diagnostic cycle. Our research provides a foundation for the clinical application of NBGS.
Bioaugmentation effect and mechanism of polycyclic aromatic hydrocarbons (PAHs) degradation using Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 under high Tween80 stress
Authors: zhang Mingle , He Zhimin , Xu XiaoYi , Ji Fan , Wang Bin 
Published: March 2024

Abstract
This study examines investigates the use of Tween80 as a typical solubilizer in the remediation of PAH-contaminated soil and explores the advantages of combined elution-microbial degradation in overcoming the inhibitory effect of high-concentration eluents. A microbial synergistic strategy system was proposed using Arthrobacter sp. SZ-3 and Pseudomonas putida B6-2 as the key bacteria in the presence of Tween80. The results indicate that in systems where Tween80 is present, the SZ-3 strain has a strong ability to degrade three kinds of PAH compounds. The B6-2 strain demonstrates multiple pathways for PAH degradation. Mixed bacteria exhibit degradation rates 60.7% higher than single bacteria at different concentrations of Tween80. Additionally, the average growth rates of mixed bacteria increased by 1.17–1.37 times, which is consistent with the changes in functional group. The detection results of protein activity within each degradation system corresponded with the growth quantity and the cyclic variation characteristics of ETS enzyme activity. Notably, the ETS of mixed bacteria was 150% higher than that of single bacteria. At a Tween80 concentration of 500 mg/L, the degradation rates of PAHs (Phe, Flu, Pyr) by mixed bacteria were 5.7%-20.7%, 8.5%-22.0%, 6.6%-22.2% higher than those of single bacteria. It was observed that the catechol 1,2-dioxygenase activity of mixed bacteria with was 2.3 times higher than of the single bacteria under the condition of 500 mg/L Tween80. The presence of Tween80 did not affect the PAH degradation pathways, but it did significantly impact the amount and duration of accumulation of the characteristic intermediate product.
Evaluating the Impact of a Trial of Labor After Cesarean Section on Labor Duration: A Retrospective Cohort Study
Authors: Ooba Hikaru , Maki Jota , Masuyama Hisashi 
Published: March 2024

Abstract

Introduction:
Repeat cesarean section (C-section) is associated with an increased risk of maternal complications and a reduction in quality of life (QOL). Trial of Labor After C-section (TOLAC) may reduce the recurrence of cesarean deliveries. Uterine rupture, a complication of TOLAC, is a serious obstetric complication, with prolonged labor duration suggested as a risk factor. However, there are no reports examining labor duration in women with cesarean scars, considering the termination of labor through cesarean section and selection bias. Our study aimed to investigate the impact of cesarean scars on the labor duration of TOLAC patients, considering these factors.
Methods:
From January 1, 2012, to December 31, 2021, we investigated a cohort of 2,964 individuals who attempted vaginal delivery at a single medical center, categorizing them into TOLAC and non-TOLAC groups. Propensity scores were generated based on factors that could influence labor duration, and survival analysis was conducted after applying Inverse Probability of Treatment Weighting (IPTW). We evaluated the robustness of the results by propensity score matching and bootstrapping as a sensitivity analysis.
Results:
Results from Cox proportional hazards regression analysis indicated that the unadjusted hazard ratio was 0.83 (95% Confidence Interval (CI): 0.70-0.98, p=0.027), whereas the IPTW-adjusted hazard ratio was 0.98 (95% CI: 0.74-1.30, p=0.91).
Conclusion:
Factors such as maternal physique and fetal characteristics influence labor duration, with the impact of cesarean scars being limited. Our findings were consistent with previous studies and provided supportive evidence.
Efficacy of the Intensive Care Unit Diary: A Mixed-Method Study Protocol
Authors: Muñoz-Rey Pilar , Romero-García Marta , Anglès-Sabaté Isabel , Ausió-Dot Albert , Alonso-Fernández Sergio , Alcalá-Jiménez Isidro , Huertas-Zurriaga Ariadna , Tur-Rubio Carlos , Delgado-Hito Pilar 
Published: March 2024

Abstract
Background: Multiple international studies report on the use of diaries in intensive care units. Some highlight the benefits for patients, others focus on family members, and few consider the vision of professionals. Most use quantitative methods. Limitations such as small sample sizes and short follow-up periods affect many studies. Therefore, further research with larger samples and a longer follow-up period, employing a mixed-methods approach is suggested. This protocol was designed to evaluate the efficacy of the use of intensive care unit diaries for patients and families, as well as to understand professionals’ experiences and satisfaction with intensive care unit diaries.
Method/Design: Mixed-method study: a quantitative phase followed by a qualitative phase.
Phase 1: Randomised, parallel, non-blinded, multicentre clinical trial, with an experimental group (diary) and a control group (no diary), to identify the efficacy of the diary in improving quality of life and preventing post-traumatic stress and anxiety/depression in patients and family. Measurement tools include the SF-36 Health Questionnaire, EIE-R scale, and HADS scale, administered 2-, 6- and 12-months post-discharge. Each group comprises 120 cases. Data analysis will use IBM-SPSS (v.24) To evaluate the satisfaction of professionals a survey will be carried out.
Phase 2: Hermeneutic phenomenological study with theoretical sampling. Size of 38-72 patients/family members until theoretical data saturation is reached. Data collection from in-depth interviews and field diary 6 months after discharge. Phenomenological analysis according to Smith. Guba and Lincoln’s rigour criteria.
Discussion: This research will provide knowledge regarding the benefits and limitations of the use of diaries, based on quantitative and qualitative data, and will promote reflection on their implementation, considering patients, family, and professionals. This study will provide new knowledge about the satisfaction of professionals with diary implementation. This multicentre study will provide valuable information on the contribution of diaries in clinical practice and their impact on patient recovery and the experiences of families and professionals. If the efficacy of the diary is confirmed, it will mark a significant advancement in the care of critically ill patients, contributing to the expansion of their use.
Trial registration: ClinicalTrials.gov Identifier: NCT05288140. First Published: March 21, 2022.
Psychosocial factors and patient and healthcare delays in large (class T3–T4) oral, oropharyngeal, and laryngeal carcinomas
Authors: Atula Markus , Atula Timo , Aro Katri , Irjala Heikki , Halme Elina , Jouppila-Mättö Anna , Koivunen Petri , Wilkman Tommy , Mäkitie Antti , Elovainio Marko , Pulkki-Råback Laura 
Published: March 2024

Abstract
Background: Psychosocial factors and socioeconomic status have been associated with incidence, survival, and quality of life among patients with head and neck cancer. We investigated the association between different psychosocial factors, socioeconomic status, and patient delays in T3–T4 oral, oropharyngeal, and laryngeal cancer.
Patients and Methods: We conducted a nationwide prospective questionnaire-based study (n = 203) over a 3-year period.
Results: We found no association between psychosocial factors (depression, social isolation, loneliness, and cynical hostility) and patient delay. Depression was three times more common among head and neck cancer patients compared with the general Finnish population. Head and neck cancer patients had lower educational levels and employment status, and were more often current smokers and heavy drinkers.
Conclusions: Although we found no association between patient delay and psychosocial factors, patients diagnosed with a large head and neck cancer appeared to have a lower socioeconomic status and higher risk for developing depression, which should be considered in clinical practice.
The Impact of Maternal Anxiety on Uterine, Umbilical Arteries Doppler Indices and Fetal growth during the second half of Ossimi pregnant sheep
Authors: Elmetwally Mohammed A. , Adlan Fatma , Samy Alaa , Rezk Shaymaa , Lasheen Samah , Hendam Basma , Orabi Heba , Shalapy Sara , Mostagir Amira 
Published: March 2024

Abstract
The objective of this prospective study was to investigate whether Doppler- and B-mode sonography could be used to assess a possible influence of maternal temperament on fetal and pregnancy-related endpoints in sheep during pregnancy. On the basis of behavioral testing related to an anxiety-eliciting stimulus (arena test), 20 sheep were divided into two groups, namely more reactive/anxious (MR: 12 sheep) and less reactive/anxious (LR: 8 sheep). After mating, several endpoints were assessed by transrectal and/or transabdominal ultrasonography every 2 weeks (wk) from breeding to wk 20 of pregnancy and blood samples were taken to evaluate the expression of angiogenic proteins at parturition. Gestational length, fetal weight and fetal numbers were also investigated. The resistance index (RI), as well as the pulsatility index (PI) of the uterine (UtA) and the fetal umbilical arteries (UMA), were measured with non-invasive Doppler sonography. Further parameters were measured with B-mode ultrasonography: diameter of amniotic vesicles (AVD; only wk 2–8), umbilicus (UMD), fetal chest (FCHD), and metacarpal length (MCL). The UtA-PI was significantly higher in MR compared to LR ewes at pregnancy between 6–12 wks (P > 0.01), while a tendency was recognized at wk 14 (P = 0.054). The same was true for UtA-RI during the first 8 wk of pregnancy (P < 0.03) when MR and LR animals were compared. Similarly, UMA-RI was higher in fetuses of MR than LR ewes at wk 14 (P < 0.0003) and 20 (P < 0.02) of pregnancy. The differences in UMA-PI reached significance at wk 6, 8, 10, and 20 (P < 0.05–0.003). Furthermore, AVD (P < 0.03, wk 2–8), FCHD (P < 0.002, wk 8–18) as well as the UMD (P < 0.054, wk 8–18) were higher in LR than MR pregnant ewes. A significant variation in the gestational length, fetal weight and fetal numbers were recorded. The expression of VEGF, NOS3 and VEGFR1 was increased in the less reactive sheep (P < 0.001). In conclusion, using Doppler- and B-mode sonography, the effects of maternal temperament on fetal growth, as well as maternal and umbilical blood flow in sheep and goats could be successfully demonstrated. The maternal anxiety affects the expression of mRNAs for VEGF, NOS3 and HIF 1α at time of parturition.
Molecular mechanism of Saikosaponin-d in the treatment of gastric cancer based on network pharmacology and in vitro experimental verification
Authors: Ning Na , Li Xiangyang , Nan Yi , Chen Guoqing , Huang Shicong , Du Yuhua , Gu Qian , Li Weiqiang , Yuan Ling 
Published: March 2024

Abstract

Aim
Network pharmacology combined with cellular experiments to research the mechanism of action of Saikosaponin-d in the treatment of gastric cancer.
Methods
Drug target genes were obtained from the PubChem database and the Swiss Target Prediction database. Additionally, target genes for gastric cancer were obtained from the GEO database and the Gene Cards database. The core targets were then identified and further analyzed through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and GESA enrichment. The clinical relevance of the core targets was assessed using the GEPIA database. Molecular docking of drug monomers and core target proteins was performed using Auto Duck Tools and Pymol software. Finally, in vitro cellular experiments including cell viability, apoptosis, cell scratch, Transwell invasion, Transwell migration, qRT-PCR, and Western blot were conducted to verify these findings of network pharmacology.
Results
The network pharmacology analysis predicted that the drug monomers interacted with 54 disease targets. Based on clinical relevance analysis, six core targets were selected: VEGFA, IL2, CASP3, BCL2L1, MMP2, and MMP1. Molecular docking results showed binding activity between the Saikosaponin-d monomer and these core targets.
Conclusion
Saikosaponin-d could inhibit gastric cancer cell proliferation, induce apoptosis, and inhibit cell migration and invasion.
Improved Human Identification by Multi-biometric Image Sensor Integration With a Deep Learning Approach
Authors: Amin Parag , D Ganesh , Gantra Amit , Singhal Priyank 
Published: March 2024

Abstract

Biometric identification technology has become a common part of daily life due to the global demand for information security and security legislation. Due to its capacity to circumvent several fundamental drawbacks of unimodal biometric systems, multimodal biometrics technology has attracted attention and grown in popularity in this respect. This research presents a novel multimodal biometric person identification system based on a VGG19 with softmax classifier (VGG19-SC) for iris and facial biometrics. The system's architecture is built on VGG19-SC, which extracts features from and categorizes images. The system was created by combining the iris and face portions of two VGG19-SC models. VGG-19 was employed to construct the well-known pertained model. A few methods, including picture augmentation and dropout techniques, were used to prevent overfitting. The VGG19-SC models were fused using feature-level and score-level fusion methods to investigate the effects of these fusion methods on recognition performance. The results demonstrated that three biometric features were more effective than two and one biometric traits in biometric identification systems. The findings similarly demonstrated the suggested method easily surpassed other cutting-edge approaches by obtaining an accuracy of 99.39% in a multi-biometric verification system.
Intranasal Curcumin ameliorates Silica induced Inflammation and Pulmonary Fibrosis via NRF-2/Keap-1 pathway and autophagy in mice model
Authors: Kumari Sneha , Singh Payal , Dash D , Singh Rashmi 
Published: March 2024

Abstract
Silicosis, one of the most serious lung fibrotic illnesses in the world is due to repeated silica dust exposures. Aberrant tissue repair and persistent inflammation in silicosis is due to occupational exposures to silica particles but specific molecular mechanism has not yet been identified. Present study was undertaken to investigate impact of silica exposures leading to lung damage and fibrosis and plausible therapeutic interventions. Here, mice model of silicosis was established where silica induced oxidative lung damage and fibrotic response has been thoroughly explored and an anti-inflammatory molecule, curcumin, derived from Curcuma longa has been for discovered for its anti-oxidant potential. In present study, curcumin was administered every alternate day for 35 days an hour prior to silica exposure and molecular mechanism of anti-inflammatory and anti-oxidative potential of curcumin was examined. Silica induced reactive oxygen species (ROS) were repressed by curcumin thereby autophagy was stimulated and deposition of damaged mitochondria were suppressed. Reduced oxidative stress resulted in reduced number of inflammatory cells, mainly neutrophils and macrophages. Curcumin treatment has reduced inflammation and the aberrant collagen repair which followed its protective benefits in mice. Curcumin also inhibited oxidants by boosting antioxidant enzyme activities by triggering Nrf2-Keap1 pathway. Autophagy markers like, PINK1, PARKIN, Cyt-c, P62/SQSTM levels were elevated in silicosis group which were significantly lowered after curcumin and dexamethasone treatment. Higher numbers of damaged mitochondria after silica inhalations were also reduced in curcumin administered groups. Curcumin initiated autophagy resulted in reduced silica-induced mitochondria-dependent apoptosis which may suggest molecular mechanism based therapeutic implications for silicosis.
Impact of S100A4 Deletion on the Macrophage Metabolome and Differentiation
Authors: Jiao Xiyao , Cui Jingwen , Li Yuhang , Li Xiangyun , Chu Zhili , Wu Xinglong 
Published: March 2024

Abstract
Alterations in the macrophage metabolome greatly influence macrophage differentiation, subsequently impacting the development of diverse clinical diseases. Although S100A4 is a crucial factor in conditioned macrophage movement and inflammatory cell recruitment, its metabolism-mediated mechanism in regulating macrophage differentiation remains unclear. Here, we generated mice with a macrophage-specific S100A4 deletion by crossing C57BL/6J-S100a4 em1(flox)Cya mice with Lyz2-cre mice. Subsequently, macrophages were isolated from these mice, and heterozygous mouse macrophages served as controls for metabolomic analysis. The S100A4 deletion significantly influenced metabolic pathways, such as those involving lysophosphatidylserine, ceramide, and L-glutamate, which are implicated in modulating macrophage differentiation. This work elucidates the metabolic intricacies associated with the S100A4-mediated regulation of macrophage differentiation and provides a valuable reference for future investigations in this field.
Analysis of risk factors for the early death of lymphocyte subsets in adult patients with secondary hemophagocytic lymphohistiocytosis
Authors: Ling Xiaosui , Chen Heng , Xu tangxing , Deng Aigen , zhang zhi , Sun Xianyi 
Published: March 2024

Abstract

Background
Hemophagocytic lymphohistiocytosis (HLH) is an excess inflammatory response syndrome caused by genetic or acquired immune dysregulation.
Method
We examined the expression levels of lymphocyte subsets in the peripheral blood of 96 patients with sHLH.
Result
This study found that CD3-CD16 + CD56 + cells < 2% and CD3 + cells < 50% were important risk factors for early mortality in sHLH patients. Furthermore, age ≥ 55 years and Hemoglobin(HGB)< 70g/L were also found to be risk factors for early mortality in sHLH patients, which is consistent with many previous studies and may indicate that our results are credible.
Conclusion
Taken together, our results suggest that the reduction of CD3-CD16 + CD56 + cells and CD3 + cells are important risk factors for early mortality in patients with sHLH. This may provide new clues for the clinical prognosis of patients with sHLH.