bluuebunny/crossref_metadata_2025_split huggingface dataset:
Klotho exerts protection in chronic kidney disease associated with regulating inflammatory response and lipid metabolism
Authors: Liu Junhui , Wang Huaicheng , Liu Qinyu , Long Shushu , Wu Yanfang , Wang Nengying , Lin Wei , Chen Gang , Lin Miao , Wen Junping 
Published: April 2024

Abstract
Background
The anti-aging protein Klotho plays a protective role in kidney disease, but its potential as a biomarker for chronic kidney disease (CKD) is controversial. Additionally, the main pathways through which Klotho exerts its effects on CKD remain unclear. Therefore, we used bioinformatics and clinical data analysis to determine its role in CKD.

Results
We analyzed the transcriptomic and clinical data from the Nephroseq v5 database and found that the Klotho gene was mainly expressed in the tubulointerstitium, and its expression was significantly positively correlated with estimated glomerular filtration rate (eGFR) and negatively correlated with blood urea nitrogen (BUN) in CKD. We further found that Klotho gene expression was mainly negatively associated with inflammatory response and positively associated with lipid metabolism in CKD tubulointerstitium by analyzing two large sample-size CKD tubulointerstitial transcriptome datasets. By analyzing 10-year clinical data from the National Health and Nutrition Examination Survey (NHANES) 2007–2016, we also found that Klotho negatively correlated with inflammatory biomarkers and triglyceride and positively correlated with eGFR in the CKD population. Mediation analysis showed that Klotho could improve renal function in the general population by modulating the inflammatory response and lipid metabolism, while in the CKD population, it primarily manifested by mediating the inflammatory response. Restricted cubic spline (RCS) analysis showed that the optimal concentration range for Klotho to exert its biological function was around 1000 pg/ml. Kaplan–Meier curves showed that lower cumulative hazards of all-cause mortality in participants with higher levels of Klotho. We also demonstrated that Klotho could reduce cellular inflammatory response and improve cellular lipid metabolism by establishing an in vitro model similar to CKD.

Conclusions
Our results suggest that Klotho exerts protection in CKD, which may be mainly related to the regulation of inflammatory response and lipid metabolism, and it can serve as a potential biomarker for CKD.
Continuous immunosuppression is required for suppressing immune responses to xenografts in non-human primate brains
Authors: Feng Su , Zhang Ting , He Zhengxiao , Zhang Wenchang , Chen Yingying , Yue Chunmei , Jing Naihe 
Published: April 2024

AbstractContinuous immunosuppression has been widely used in xenografts into non-human primate brains. However, how immune responses change after transplantation in host brains under continuous immunosuppressive administration and whether immunosuppression can be withdrawn to mitigate side effects remain unclear. Human induced neural stem/progenitor cells (iNPCs) have shown long-term survival and efficient neuronal differentiation in primate brains. Here, we evaluate the immune responses in primate brains triggered by human grafts. The results show that the immune responses, including the evident activation of microglia and the strong infiltration of lymphocytes (both T- and B-cells), are caused by xenografts at 4 months post transplantation (p.t.), but significantly reduced at 8 months p.t. under continuous administration of immunosuppressant Cyclosporin A. However, early immunosuppressant withdrawal at 5 months p.t. results in severe immune responses at 10 months p.t. These results suggest that continuous long-term immunosuppression is required for suppressing immune responses to xenografts in primate brains.
Evaluation on Properties of Cement Mortar and Brick Using Magnetically Separated Coal Power Plant Bottom Ash
Authors: Kim Ji-Hyun , Moon Hoon , Chung Chul-Woo 
Published: April 2024

AbstractRecycling of abandoned waste bottom ash has been a key issue in Republic of Korea in terms of environmental protection as well as economic concern. In this work, a method for recycling of abandoned bottom ash has been discussed based on the results from laboratory and industrial-scale experiments. Abandoned bottom ash was magnetically separated and properties of magnetically separated bottom ash samples as well as properties of mortar and masonry cement brick made of bottom ash were investigated. According to the experimental results, bulk and skeletal densities were ranked in the order of strongly magnetic > weakly magnetic > as-received > non-magnetic (from heavier to lighter) bottom ash. From laboratory-scale experiments, compressive strengths of mortars made of bottom ash samples (measured by ASTM C 109) were lower than that of mortar made of standard sand. Among bottom ash samples, mortar made of non-magnetic bottom ash (after removal of unburnt carbon) showed higher compressive strength with lower thermal conductivity (measured by ASTM C 1113) and weight than others. Masonry cement brick made of magnetic bottom ash showed lower weight and thermal conductivity than those made of standard sand, while meeting the KS strength guideline as a masonry cement brick. The results suggest the applicability of bottom ash as lightweight aggregate for production of masonry cement brick. However, considering the lower strength obtained from masonry cement brick made of as-received bottom ash (without removal of unburnt carbon), unburnt carbon content should be removed prior to its utilization as lightweight aggregate.
Probiotic Pediococcus pentosaceus restored gossypol-induced intestinal barrier injury by increasing propionate content in Nile tilapia
Authors: Ding Feifei , Zhou Nannan , Luo Yuan , Wang Tong , Li Weijie , Qiao Fang , Du Zhenyu , Zhang Meiling 
Published: April 2024

Abstract
Background
Intestinal barrier is a dynamic interface between the body and the ingested food components, however, dietary components or xenobiotics could compromise intestinal integrity, causing health risks to the host. Gossypol, a toxic component in cottonseed meal (CSM), caused intestinal injury in fish or other monogastric animals. It has been demonstrated that probiotics administration benefits the intestinal barrier integrity, but the efficacy of probiotics in maintaining intestinal health when the host is exposed to gossypol remains unclear. Here, a strain (YC) affiliated to Pediococcus pentosaceus was isolated from the gut of Nile tilapia (Oreochromis niloticus) and its potential to repair gossypol-induced intestinal damage was evaluated.

Results
A total of 270 Nile tilapia (2.20 ± 0.02 g) were allotted in 3 groups with 3 tanks each and fed with 3 diets including CON (control diet), GOS (control diet containing 300 mg/kg gossypol) and GP (control diet containing 300 mg/kg gossypol and 108 colony-forming unit (CFU)/g P. pentosaceus YC), respectively. After 10 weeks, addition of P. pentosaceus YC restored growth retardation and intestinal injury induced by gossypol in Nile tilapia. Transcriptome analysis and siRNA interference experiments demonstrated that NOD-like receptors (NLR) family caspase recruitment domain (CARD) domain containing 3 (Nlrc3) inhibition might promote intestinal stem cell (ISC) proliferation, as well as maintaining gut barrier integrity. 16S rRNA sequencing and gas chromatography-mass spectrometry (GC-MS) revealed that addition of P. pentosaceus YC altered the composition of gut microbiota and increased the content of propionate in fish gut. In vitro studies on propionate’s function demonstrated that it suppressed nlrc3 expression and promoted wound healing in Caco-2 cell model.

Conclusions
The present study reveals that P. pentosaceus YC has the capacity to ameliorate intestinal barrier injury by modulating gut microbiota composition and elevating propionate level. This finding offers a promising strategy for the feed industry to incorporate cottonseed meal into fish feed formulations.

Graphical Abstract
Exploring the connotation of “Local Community” in ICOMOS discourse: a textual analysis based on authoritative documents
Authors: Xu Yishu , Tang Yuexing , Zhang Luchen 
Published: April 2024

Abstract
As an authoritative institution in cultural heritage conservation, ICOMOS plays a crucial role in guiding local communities’ participation in heritage conservation. However, its scattered and vague descriptions of local communities pose significant obstacles to further research and practice of community participation in heritage conservation. Given the increasing importance of local communities in heritage conservation, it is essential to systematically explore the connotation of Local Community connotations within ICOMOS discourse. This research employs Natural Language Processing methods to analyze ICOMOS’s descriptions of Local Community. Utilizing computational techniques of word frequency calculation, LDA (Latent Dirichlet Allocation) topic model keyword calculation, and hierarchical clustering calculation, the research uncovers relevant keywords and its thematic clusters of Local Community. These findings are further elucidated by aligning them with the principles outlined in authoritative documents of ICOMOS. The research indicates that ICOMOS’s descriptions of Local Community can be summarized into four main keywords families. These keywords families can be summarized as a comprehensive Local Community “three-level, four-family” keywords system. The “Tourism” keywords family exhibits a close association with Local Community, highlighting ICOMOS’s heightened emphasis on heritage tourism. The “Management-Development” keywords family occupies the second level, emphasizing fundamental principles for local communities’ participation in heritage practices. The “Traditional-Knowledge” and “Social-Economic” Keywords Families, situated in the third level, respectively describe the value attributes and conservation approaches of local communities. Each keywords family formed over different periods, exhibiting varying trends of development. By systematically integrating ICOMOS’s descriptions of Local Community and employing Natural Language Processing for in-depth exploration, This research aims to construct a cognitive understanding of local communities from a new perspective of quantitative text analysis, with the intention of providing theoretical references for subsequent research on local communities.
Identifying factors influencing the spatial distribution of minority cultural heritage in Southwest China
Authors: Li Chang , Qian Yuyao , Li Zhaokun , Tong Tong 
Published: April 2024

Abstract
Minority Cultural Heritage (MCH) plays a crucial role in preserving human cultural and historical diversity. In Southwest China, there is a disparity between the abundance of ethnic minority cultural heritage resources and their inadequate protection and development. However, limited by interdisciplinary barriers, research into the distribution patterns of varied MCH in this area remains unexplored, making an initial step towards comprehensive preservation. The study investigates the relationship between MCH distribution and its association with factors of geography, climate, transportation, economics, and demographics, utilizing spatial geographic analysis, Geodetector methods, and social network analysis. The results indicate the following: (1) The distribution of 483 national-level MCH in Southwest China exhibits clustering, with a higher concentration in the southern regions. Honghe Prefecture, Dali Prefecture, and Qiandongnan Prefecture serve as the core areas with high MCH density, accounting for 47.2% of the total concentration. (2) All three categories of MCH show cohesive distribution patterns. cultural heritage clusters in the northern to southeastern regions of Yunnan Province, intangible cultural heritage clusters in the southeastern to southern regions of Guizhou Province, and agricultural cultural heritage clusters in the northern region of Yunnan Province. (3) The distribution of MCH among different ethnic minorities demonstrates polarization. The Yi and Tibetan ethnic groups have a higher quantity and diversity of MCH, while the Qiang, Lisu, and Jingpo ethnic groups have relatively fewer resources. (4) Single-factor analysis reveals that natural factors like the proportion of mountainous areas, river density, and annual average sunshine, as well as human factors like the proportion of minority population, urbanization rate, and road mileage, have the strongest explanatory power for the distribution of MCH. Furthermore, the interaction between these factors and others enhances the explanatory power for the distribution of MCH in Southwest China. This study provides scientific evidence for the assessment, protection, and sustainable development of MCH.
Distribution of eukaryotic environmental DNA in global subseafloor sediments
Authors: Hoshino Tatsuhiko , Inagaki Fumio 
Published: April 2024

AbstractThe analysis of eukaryotic environmental DNA (eDNA) in sediment has the potential for understanding past ecosystems, even for taxa lacking skeletons or preserved only as a part of necromass. Despite the paleoenvironmental and ecological importance of eukaryotic eDNA in marine sediment, the duration of remaining eDNA and the species of eDNA present in marine sediment has not been well investigated. Here, we analyzed eDNA extracted from 299 sediment samples down to 678 m below the seafloor at 40 geologically distinct sites. The results showed that eukaryotic eDNA was amplified from more than 80% of the sediments with a depositional age of less than 100,000 years. The eDNA was well conserved in anoxic sediments than in oxic sediments, with PCR success rates of 48% and 18%, respectively. The eukaryotic communities include non-benthic organisms such as marine plankton, including diatoms, dinoflagellates, and coccolithophores. A freshwater diatom genus Aulacoseira was detected in the Baltic Sea sediments from the last glacial lacustrine environment. These results provide new insights into the global-scale distribution of the past eukaryotic eDNA preserved in marine sediment.
A case of gastric adenocarcinoma with pyloric gland-type infiltrating submucosa
Authors: Hirata Kaiho , Yagi Shusuke , Miyazaki Hideki , Yamada Kazuhiko , Akazawa Naoki , Enomoto Naoki , Nohara Kyoko , Yokoi Chizu , Igari Toru , Kokudo Norihiro 
Published: April 2024

Abstract
Background
The development of immunohistochemical staining has revealed that gastric adenocarcinoma with the gastric phenotype can be divided into the foveolar, fundic gland, and pyloric gland phenotypes. Gastric adenocarcinoma of the pyloric gland type is difficult to diagnose using biopsy because of its low atypia and rarity. Herein, we describe a case of gastric adenocarcinoma of the pyloric gland type that was diagnosed immunohistochemically after endoscopic resection.

Case presentation
A 67-year-old man was referred to our hospital for the diagnosis and treatment of a 30-mm elevated lesion on the lesser curvature side of the middle of the gastric body. Although four biopsies were performed, it was difficult to determine whether the lesion was benign or malignant. Therefore, endoscopic submucosal dissection was performed, and the presence of tumor cells infiltrating the submucosa with venous invasions was identified. Immunohistochemical staining revealed that the tumor cells were positive for MUC5AC and MUC6 and negative for Pepsinogen I and H + /K + -ATPase. From the above findings, he was diagnosed as having gastric adenocarcinoma with pyloric gland type. The patient underwent a laparoscopic distal gastrectomy and was discharged without any adverse events.

Conclusions
Gastric adenocarcinoma of the pyloric gland type is a rare disease, and endoscopic resection can serve as a viable diagnostic option for this condition when it is difficult to diagnose using biopsy. Immunohistochemical pathology images can aid in the diagnosis of gastric adenocarcinoma of the pyloric gland type.
Parenting practices and interventions during the COVID-19 pandemic lockdown: an exploratory cross-sectional study of caregivers in Brazil, Mexico, and the United States
Authors: Juras Mariana M. , Coelho Acileide Cristiane F. , Vázquez Alejandro L. , Ribeiro Michela , Kohlsdorf Marina , Custódio Alice Lima , Amador Buenabad Nancy G. , Perez Lucia Vazquez , Hooley Cole , Barnett Miya L. , Baumann Ana A. 
Published: April 2024

Abstract
Introduction
The COVID-19 pandemic led countries’ governments to rapidly establish lockdowns and social distancing, which altered family routines and the quality of family relationships worldwide.

Objectives
This exploratory cross-sectional study aimed to identify the impacts of the social distancing and lockdown in parenting practices of caregivers from Brazil, Mexico, and the USA, and to analyze the continuity of parenting intervention support for children and their families at the beginning of the pandemic in these countries.

Methods
The sample consisted of 704 caregivers of children (286 from Brazil, 225 from Mexico, and 193 from the USA) who answered an online survey about parenting practices before/after quarantine, caregiver/child routines, feelings related to quarantine, changes in everyday life since the beginning of the COVID-19 pandemic, contact with health professionals, and sources of parenting information.

Results
Data indicate that caregivers from the three countries experienced similar parenting practices during this time, and did not report significant changes before and after the lockdown. They sought information about parenting predominantly via social media. Those receiving previous mental health care perceived the transition from in-person to telehealth services during the pandemic as feasible and acceptable.

Conclusion
This study will be helpful for clinicians and parents to contextualize their practices amid long-standing effects that the COVID-19 pandemic can have on children and their families during and post-pandemic from multiple cultural backgrounds.
Who speaks for the university? Social fiction as a lens for reimagining higher education futures
Authors: Mishra Punya , Oster Nicole , Wagner Phoebe 
Published: April 2024

AbstractThis paper combines social fiction and academic analysis to envision hopeful futures for higher education. At the heart of the exploration is Phoebe Wagner’s speculative fiction piece, University, Speaking, which personifies a university grappling with environmental, political, and social change. Phoebe Wagner’s first-person narrative highlights the power of collective voice, the importance of centering community, and the urgent need to cultivate resilience and adaptability. Through analysis of key themes, this paper connects Phoebe Wagner’s fictional vision to contemporary research on the multi-faceted and complex challenges facing universities today. By integrating artistic and academic perspectives, this paper discusses new possibilities for universities navigating disruption and change.
Access to essential medicines for diabetes care: availability, price, and affordability in central Ethiopia
Authors: Deressa Hachalu Dugasa , Abuye Habtamu , Adinew Alemayehu , Ali Mohammed K. , Kebede Tedla , Habte Bruck Messele 
Published: April 2024

Abstract
Background
Diabetes is a major global public health burden. Effective diabetes management is highly dependent on the availability of affordable and quality-assured essential medicines (EMs) which is a challenge especially in low-and-middle-income countries such as Ethiopia. This study aimed to assess the accessibility of EMs used for diabetes care in central Ethiopia’s public and private medicine outlets with respect to availability and affordability parameters.

Methods
A cross-sectional study was conducted in 60 selected public and private medicine outlets in central Ethiopia from January to February 2022 using the World Health Organization/Health Action International (WHO/HAI) standard tool to assess access to EMs. We included EMs that lower glucose, blood pressure, and cholesterol as these are all critical for diabetes care. Availability was determined as the percentage of surveyed outlets per sector in which the selected lowest-priced generic (LPG) and originator brand (OB) products were found. The number of days’ wages required by the lowest paid government worker (LPGW) to purchase a one month’s supply of medicines was used to measure affordability while median price was determined to assess patient price and price markup difference between public procurement and retail prices.

Results
Across all facilities, availability of LPG and OB medicines were 34.6% and 2.5% respectively. Only two glucose-lowering (glibenclamide 5 mg and metformin 500 mg) and two blood pressure-lowering medications (nifedipine 20 mg and hydrochlorothiazide 25 mg) surpassed the WHO’s target of 80% availability. The median price based on the least measurable unit of LPG diabetes EMs was 1.6 ETB (0.033 USD) in public and 4.65 ETB (0.095 USD) in private outlets. The cost of one month’s supply of diabetes EMs was equivalent to 0.3 to 3.1 days wages in public and 1.0 to 11.0 days wages in private outlets, respectively, for a typical LPGW. Thus, 58.8% and 84.6% of LPG diabetes EMs included in the price analysis were unaffordable in private and public outlets, respectively.

Conclusions
There are big gaps in availability and affordability of EMs used for diabetes in central Ethiopia. Policy makers should work to improve access to diabetes EMs. It is recommended to increase government attention to availing affordable EMs for diabetes care including at the primary healthcare levels which are more accessible to the majority of the population. Similar studies are also recommended to be conducted in different parts of Ethiopia.
Concepts of illness etiology in a traditional medical system: analysis of philosophy of Aruh and healing ritual as ethnomedicine
Authors: Anshari Muhammad 
Published: April 2024

AbstractThe process of recovering from a disease can vary among ethnic groups, culminating in distinct medical systems. Indonesia, an archipelagic country with over a thousand ethnicities, requires extensive research on ethnomedicine. A critical aspect of this research is illness etiology, which plays a crucial role in understanding the concept of prevention, therapy, and treatment developed by these ethnic groups. An adequate understanding of illness etiology will facilitate formulating a powerful, superior traditional medical system and provide practical benefits to assist local community health efforts. Therefore, this research aims to describe illness etiology by analyzing the philosophy of life: aruh and healing ritual according to the ethnomedicine of the Dayak Paramasan in Meratus Mountains. The method used was ethnography, which involved two traditional healing practitioners. Based on the results, illness were caused by behavior that was not aruh (disharmonious) with the environment. The types of illness include alamiah (physiological), perbuatan (psychosocial), and kapuhunan (socio-cultural). Meanwhile, illness therapy with ritual aims to return the aruh (harmonious, intimate, and peaceful) living conditions between the soul of the sufferer and the natural environment.