bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stickiness: A New Variable to Characterize the Temperature and Humidity Contributions toward Humid Heat
Authors: Ivanovich Catherine C. , Sobel Adam H. , Horton Radley M. , Raymond Colin 
Published: March 2024

Abstract
Extreme wet-bulb temperatures (Tw) are often used as indicators of heat stress. However, humid heat extremes are fundamentally compound events, and a given Tw can be generated by various combinations of temperature and humidity. Differentiating between extreme humid heat driven by temperature versus humidity is essential to identifying these extremes’ physical drivers and preparing for their distinct impacts. Here we explore the variety of combinations of temperature and humidity contributing to humid heat experienced across the globe. In addition to using traditional metrics, we derive a novel thermodynamic state variable named “stickiness.” Analogous to the oceanographic variable “spice” (which quantifies the relative contributions of temperature and salinity to a given water density), stickiness quantifies the relative contributions of temperature and specific humidity to a given Tw. Consistent across metrics, we find that high magnitudes of Tw tend to occur in the presence of anomalously high moisture, with temperature anomalies of secondary importance. This widespread humidity dependence is consistent with the nonlinear relationship between temperature and specific humidity as prescribed by the Clausius–Clapeyron relationship. Nonetheless, there is a range of stickiness observed at moderate-to-high Tw thresholds. Stickiness allows a more objective evaluation of spatial and temporal variability in the temperature versus humidity dependence of humid heat than traditional variables. In regions with high temporal variability in stickiness, predictive skill for humid heat-related impacts may improve by considering fluctuations in atmospheric humidity in addition to dry-bulb temperature.

Significance Statement
Extreme humid heat increases the risk of heat stress through its influence over humans’ ability to cool down by sweating. Understanding whether humid heat extremes are generated more due to elevated temperature or humidity is important for identifying factors that may increase local risk, preparing for associated impacts, and developing targeted adaptation measures. Here we explore combinations of temperature and humidity across the globe using traditional metrics and by deriving a new variable called “stickiness.” We find that extreme humid heat at dangerous thresholds occurs primarily due to elevated humidity, but that stickiness allows for thorough analysis of the drivers of humid heat at lower thresholds, including identification of regions prone to low- or high-stickiness extremes.
Stress relaxation behavior of graphene-epoxy nanocomposites: Effects of graphene fraction, strain levels, and temperature
Authors: Acar Alperen , Bakbak Okan , Colak Ozgen 
Published: March 2024

In this study, strain level and temperature-dependent stress relaxation behavior of neat epoxy and graphene-epoxy nanocomposites are experimentally investigated. Tests are performed for neat epoxy, graphene-epoxy nanocomposite with content of 0.1 wt% and 0.5 wt% graphene. To determine the effect of strain levels on relaxation behavior, three constant strain levels are chosen: 3.16%, 7.15%, and 35.5%. Those strain levels are chosen to correspond to different zones on the stress–strain curves as: viscoelastic region, yield region, and visco-plastic region. Results have shown that with the addition of 0.1 wt% graphene, relaxation modulus improved 41% at 3.16% constant strain. The effect of graphene is less effective at high strain values: for 7.15% strain, the change in relaxation modulus is 32%, and at 35.5 %strain, the change is 21.25%. In addition, tests are performed for three temperature values: 23, 65, and 120°C to determine the effect of temperature on the relaxation behavior. As expected, nano composition has an improved effect on the stress relaxation behavior. An increasing temperature increases the stress relaxation values. At room temperature (for 7.15% constant strain level) the drop in relaxation modulus for neat epoxy is 32%, this value drops to 22% with the addition of 0.1 wt% graphene. At 65°C, the rate of decrease in modulus with the addition of graphene is almost unchanged. At 120°C, the relaxation performance of the material slightly decreases. Therefore, as a novel result of the research, it is determined through the conducted experiments that increasing temperature lessens the improvement effect of graphene composition on the stress relaxation behavior.
Investigating the influence of annealing and nozzle diameter on tensile strength of polyethylene terephthalate glycol composites
Authors: Raj Tapish , Tyagi Bobby , Jain Akash , Sahai Ankit , Sharma Rahul Swarup 
Published: March 2024

Additive Manufacturing (AM) techniques, particularly Fused Filament Fabrication (FFF), have revolutionized prototyping and low-volume production. Improving the tensile properties of FFF-printed parts is a primary objective to elevate their functional utility. This study aimed to investigate the effects of annealing time (ATM), annealing temperature (ATP), and nozzle diameter (ND) on the tensile strength (TS) of two commonly used printing materials: Polyethylene Terephthalate Glycol (PETG) and PETG reinforced with carbon fibre (PETG-CF). Samples with varying ND (0.4 mm, 0.6 mm, and 0.8 mm) underwent annealing at ATP of 80°C and 100°C for ATM of 60 min and 120 min, respectively. Subsequent tensile tests were meticulously conducted, and regression models were employed to comprehensively analyse the influence of these control factors on TS. The findings from the tensile tests on annealed specimens revealed substantial improvements in TS for both PETG and PETG-CF materials. Statistical analysis, Taguchi method (TM), and response surface methodology (RSM) indicated that ND exerted a more pronounced impact on TS compared to ATM and ATP. By identifying the optimal control factor combinations for each material, the study pinpointed that the best TS was achieved at 0.8 mm ND, 120 minutes ATM, and 100°C ATP for PETG-CF. The remarkable enhancement in tensile properties for annealed FFF-printed parts underscores the potential of PETG-CF to replace structural metallic components in critical applications within the automotive and aeronautical industries.
Oral anticancer medicine interventions: A cross-sectional study in French community pharmacies
Authors: Macé Florent , Peyron Christine , Cransac Amélie , Pistre Pauline , Boulin Mathieu 
Published: March 2024

Introduction The increasing number of oral anticancer medicines (OAMs) dispensed in community pharmacies and the associated challenges (misuse, management of side effects) give the community pharmacist (CP) a major role in the pharmacotherapeutic management of cancer patients. In France, as a response to these challenges, cancer outpatients can schedule a meeting with their CP to ensure the safe and effective use of OAMs. The objectives of this study were to evaluate the perspectives of these interventions regarding their implementation and the opinion of French CPs. Methods A declarative survey and semi-structured interviews were conducted with CPs that dispensed at least one OAM between January 2021 and March 2022. The study was conducted between April and August 2022. Results Eighty-five CPs completed the survey. Of these pharmacists, 21% ( n = 18) had already performed OAM interventions and 91% ( n = 61) wanted to implement them. Lack of time, knowledge and training were the main barriers to implementation. No correlations were identified between the characteristics of community pharmacies and the likelihood of implementing OAM interventions. Conclusions Considering that CPs seem willing to implement them and the favourable context in France, this observational study highlights the potential of OAM interventions to improve the management of cancer patients. Though further studies are required to better evaluate the implementation and the potential effects of these interventions, OAM interventions could be relevant strategies in other healthcare systems to secure the management of cancer patients through the involvement of the CP.
In vivo ablation of NF-κB cascade effectors alleviates disease burden in myeloproliferative neoplasms
Authors: Laranjeira Angelo B. A. , Kong Tim , Snyder Steven C. , Fulbright Mary C. , Fisher Daniel A. C. , Starczynowski Daniel T. , Oh Stephen T. 
Published: March 2024

Abstract
Hyperactivation of the NF-κB cascade propagates oncogenic signaling and proinflammation, which together augments disease burden in myeloproliferative neoplasms (MPNs). Here, we systematically ablate NF-κB signaling effectors to identify core dependencies using a series of primary samples and syngeneic and patient–derived xenograft (PDX) mouse models. Conditional knockout of Rela attenuated Jak2V617F- and MPLW515L-driven onset of polycythemia vera and myelofibrosis disease hallmarks, respectively. In PDXs, RELA knockout diminished leukemic engraftment and bone marrow fibrosis while extending survival. Knockout of upstream effector Myd88 also alleviated disease burden; conversely, perturbation of negative regulator miR-146a microRNA induced earlier lethality and exacerbated disease. Perturbation of NF-κB effectors further skewed the abundance and distribution of hematopoietic multipotent progenitors. Finally, pharmacological targeting of interleukin-1 receptor–associated kinase 4 (IRAK4) with inhibitor CA-4948 suppressed disease burden and inflammatory cytokines specifically in MPN without inducing toxicity in nondiseased models. These findings highlight vulnerabilities in MPN that are exploitable with emerging therapeutic approaches.
T-cell help in the tumor microenvironment enhances rituximab-mediated NK-cell ADCC
Authors: Arora Jyoti , Ayyappan Sabarish , Yin Chaobo , Smith Brian J. , Lemke-Miltner Caitlin D. , Wang Zhaoming , Farooq Umar , Weiner George J. 
Published: March 2024

Abstract
Rituximab (RTX) and other monoclonal antibodies (mAbs) that bind directly to malignant cells are of great clinical value but are not effective for all patients. A major mechanism of action of RTX is antibody-dependent cellular cytotoxicity (ADCC) mediated by natural killer (NK) cells. Prior in vitro studies in our laboratory demonstrated that T cells contribute to maintaining the viability and cytotoxic potential of NK cells activated by anti-CD20–coated target B cells. Here, we conducted studies using a novel mouse model and clinical correlative analysis to assess whether T-cell help contribute to RTX-mediated NK-cell ADCC in the tumor microenvironment (TME) in vivo. A humanized mouse model was developed using Raji lymphoma cells and normal donor peripheral blood mononuclear cells that allows for control of T-cell numbers in the lymphoma TME. In this model, NK-cell viability and CD16 and CD25 expression dropped after RTX in the absence of T cells but increased in the presence of T cells. RTX therapy was more effective when T cells were present and was ineffective when NK cells were depleted. In patients with indolent lymphoma, fine needle aspirates were obtained before and ∼1 week after treatment with a RTX-containing regimen. There was a strong correlation between CD4+ T cells as well as total T cells in the pretherapy TME and an increase in NK-cell CD16 and CD25 expression after RTX. We conclude that T-cell help in the TME enhances RTX-mediated NK-cell viability and ADCC.
Confidence intervals for validation statistics with data truncation in genomic prediction
Authors: Bermann Matias , Legarra Andres , Munera Alejandra Alvarez , Misztal Ignacy , Lourenco Daniela 
Published: March 2024

Abstract
Background
Validation by data truncation is a common practice in genetic evaluations because of the interest in predicting the genetic merit of a set of young selection candidates. Two of the most used validation methods in genetic evaluations use a single data partition: predictivity or predictive ability (correlation between pre-adjusted phenotypes and estimated breeding values (EBV) divided by the square root of the heritability) and the linear regression (LR) method (comparison of “early” and “late” EBV). Both methods compare predictions with the whole dataset and a partial dataset that is obtained by removing the information related to a set of validation individuals. EBV obtained with the partial dataset are compared against adjusted phenotypes for the predictivity or EBV obtained with the whole dataset in the LR method. Confidence intervals for predictivity and the LR method can be obtained by replicating the validation for different samples (or folds), or bootstrapping. Analytical confidence intervals would be beneficial to avoid running several validations and to test the quality of the bootstrap intervals. However, analytical confidence intervals are unavailable for predictivity and the LR method.

Results
We derived standard errors and Wald confidence intervals for the predictivity and statistics included in the LR method (bias, dispersion, ratio of accuracies, and reliability). The confidence intervals for the bias, dispersion, and reliability depend on the relationships and prediction error variances and covariances across the individuals in the validation set. We developed approximations for large datasets that only need the reliabilities of the individuals in the validation set. The confidence intervals for the ratio of accuracies and predictivity were obtained through the Fisher transformation. We show the adequacy of both the analytical and approximated analytical confidence intervals and compare them versus bootstrap confidence intervals using two simulated examples. The analytical confidence intervals were closer to the simulated ones for both examples. Bootstrap confidence intervals tend to be narrower than the simulated ones. The approximated analytical confidence intervals were similar to those obtained by bootstrapping.

Conclusions
Estimating the sampling variation of predictivity and the statistics in the LR method without replication or bootstrap is possible for any dataset with the formulas presented in this study.
Modulation of early gene expression responses to water deprivation stress by the E3 ubiquitin ligase ATL80: implications for retrograde signaling interplay
Authors: Méndez-Gómez Manuel , Sierra-Cacho Daniel , Jiménez-Morales Estela , Guzmán Plinio 
Published: March 2024

Abstract
Background
Primary response genes play a pivotal role in translating short-lived stress signals into sustained adaptive responses. In this study, we investigated the involvement of ATL80, an E3 ubiquitin ligase, in the dynamics of gene expression following water deprivation stress. We observed that ATL80 is rapidly activated within minutes of water deprivation stress perception, reaching peak expression around 60 min before gradually declining. ATL80, despite its post-translational regulation role, emerged as a key player in modulating early gene expression responses to water deprivation stress.

Results
The impact of ATL80 on gene expression was assessed using a time-course microarray analysis (0, 15, 30, 60, and 120 min), revealing a burst of differentially expressed genes, many of which were associated with various stress responses. In addition, the diversity of early modulation of gene expression in response to water deprivation stress was significantly abolished in the atl80 mutant compared to wild-type plants. A subset of 73 genes that exhibited a similar expression pattern to ATL80 was identified. Among them, several are linked to stress responses, including ERF/AP2 and WRKY transcription factors, calcium signaling genes, MAP kinases, and signaling peptides. Promoter analysis predicts enrichment of binding sites for CAMTA1 and CAMTA5, which are known regulators of rapid stress responses. Furthermore, we have identified a group of differentially expressed ERF/AP2 transcription factors, proteins associated with folding and refolding, as well as pinpointed core module genes which are known to play roles in retrograde signaling pathways that cross-referenced with the early ATL80 transcriptome.

Conclusions
Based on these findings, we propose that ATL80 may target one or more components within the retrograde signaling pathways for degradation. In essence, ATL80 serves as a bridge connecting these signaling pathways and effectively functions as an alarm signal.
Clarifying the biological and statistical assumptions of cross-sectional biological age predictors: an elaborate illustration using synthetic and real data
Authors: Sluiskes Marije H. , Goeman Jelle J. , Beekman Marian , Slagboom P. Eline , Putter Hein , Rodríguez-Girondo Mar 
Published: March 2024

Abstract
Background
There is divergence in the rate at which people age. The concept of biological age is postulated to capture this variability, and hence to better represent an individual’s true global physiological state than chronological age. Biological age predictors are often generated based on cross-sectional data, using biochemical or molecular markers as predictor variables. It is assumed that the difference between chronological and predicted biological age is informative of one’s chronological age-independent aging divergence ∆.

Methods
We investigated the statistical assumptions underlying the most popular cross-sectional biological age predictors, based on multiple linear regression, the Klemera-Doubal method or principal component analysis. We used synthetic and real data to illustrate the consequences if this assumption does not hold.

Results
The most popular cross-sectional biological age predictors all use the same strong underlying assumption, namely that a candidate marker of aging’s association with chronological age is directly informative of its association with the aging rate ∆. We called this the identical-association assumption and proved that it is untestable in a cross-sectional setting. If this assumption does not hold, weights assigned to candidate markers of aging are uninformative, and no more signal may be captured than if markers would have been assigned weights at random.

Conclusions
Cross-sectional methods for predicting biological age commonly use the untestable identical-association assumption, which previous literature in the field had never explicitly acknowledged. These methods have inherent limitations and may provide uninformative results, highlighting the importance of researchers exercising caution in the development and interpretation of cross-sectional biological age predictors.
Arthritis prevalence is associated with metabolic syndrome risk factors but not with physical activity in middle-aged and older patients - a cross-sectional study
Authors: Qiu Fanji , Li Jinfeng , Gan Liaoyan , Legerlotz Kirsten 
Published: March 2024

Abstract
Background
In light of the aging population, increasingly suffering from the metabolic syndrome (MS), strategies need to be developed to address global public health challenges known to be associated with MS such as arthritis. As physical activity (PA) may play a crucial role in tackling those challenges, this study aimed to determine the association between the number of MS risk factors, PA and arthritis in people ≥ 50 years old.

Methods
Data from the Survey of Health, Ageing, and Retirement in Europe (SHARE) were used to estimate the prevalence of arthritis and MS risk factors in the European population ≥ 50 years and to evaluate the associations between MS risk factors, PA and arthritis. Binary logistic regression was performed to calculate the odds ratio of different factors.

Results
73,125 participants were included in the analysis. 55.75% of patients stated at least one of the three MS risk factors. The prevalence of rheumatoid arthritis (RA) and osteoarthritis (OA)/other rheumatism among ≥ 50 years population was 10.19% and 19.32% respectively. Females showed a higher prevalence of arthritis than males. Prevalence did not differ between groups with different levels of PA. Arthritis prevalence was positively correlated with the number of MS risk factors (P < 0.01) but not with PA (P > 0.05).

Conclusion
Middle-aged and older Europeans with multiple comorbidities suffered from RA, OA or other rheumatism more frequently than participants with fewer comorbidities, while the level of physical activity was not associated with the number of metabolic risk factors in patients with RA and OA/other rheumatism.
A systematic review and meta-analysis of clinical efficacy of early and late rehabilitation interventions for ischemic stroke
Authors: Wei Xufang , Sun Shengtong , Zhang Manyu , Zhao Zhenqiang 
Published: March 2024

Abstract
Introduction
At present, stroke has become the first cause of death and disability among Chinese adults. With the coming of the aging population in China, the disease burden brought by stroke will be increasingly aggravated. And stroke is a leading cause of disability. There is a golden plastic period after stroke, during which timely and safe intervention and rehabilitation therapy can effectively improve the disability status. However, there is still controversy about the duration of interventional rehabilitation after stroke. This study conducted a meta-analysis on the influence of intervention in early and late ischemic stroke rehabilitation.

Method
Chinese language databases such as CNKI, Wanfang, and VIP, and English language databases such as Embase, PubMed, Web of Science, and The Cochrane Library were searched, and RCT related to early and late rehabilitation of ischemic stroke from the establishment of the database to October 2023 was collected. Review Manager 5.4.1 was used for relevant analysis. The main outcomes were Barthel Index or Modified Barthel Index, Fugl-Meyer Assessment scale, NIHSS, China Stroke Scale. Standardized Mean Difference (SMD) was used as an effective indicator of continuity variables, and the estimated interval was expressed by 95% confidence interval (CI).

Results
A total of 1908 patients were included in 16 studies. The results showed that, compared with late rehabilitation, early rehabilitation improved clinical efficacy. Barthel Index or Modified Barthel Index score was [SMD = 1.40, 95%CI(1.16,1.63), p < 0.001]; the score of Fugl-Meyer Assessment Scale was [SMD = 1.18, 95%Cl (0.85, 1.52), P < 0.001]; the score of NIHSS was [SMD= -0.44, 95% CI(-0.65, -0.24), P < 0.001]; the result of China Stroke Scale score was [SMD= -0.37, 95%CI(-0.56, -0.18), P < 0.001].

Conclusion
In comparison with late rehabilitation, early rehabilitation can significantly improve self-care abilities, daily activities, and neurological functions of ischemic stroke patients.

Trial registration
This meta-analysis has been registered with Prospero, and the registration number is CRD42022309911. The registration period is March 22, 2022.