bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of treatment with two intravaginal inserts on the uterine and vaginal microflora of early postpartum beef cows
Authors: PADULA AM , MACMILLAN KL 
Published: June 2007

Objective To describe the bacterial species found within the uterus and vagina of early postpartum (10 to 20 d) beef cows treated for 14 d with (i) two intravaginal (CIDR‐BTM) progesterone releasing inserts (C‐P4; n=31); (ii) two identical but blank inserts (C‐BL; n=15); (iii) untreated controls (CON; n=15). It was hypothesised that due to the locally immunosuppressive effects of progesterone on the uterus, the bacterial microflora of C‐P4 would be altered by this treatment in contrast to CON and C‐BL. Procedure Cows were enrolled at two intervals 7 d apart. Blood samples were collected at 0, 7 and 14 d after beginning treatments for subsequent progesterone assay. A triple guarded swabbing technique was used to collect bacteriological samples from the uterus of every cow on days 0, 7 and 14 following CIDR™ insertion. Swabs were also collected from the inserts and vagina of every cow on day 14. Due to the small sample sizes, only descriptive statistics were generated. Results Plasma progesterone levels were maintained at mid‐luteal phase concentrations by the intravaginal progesterone releasing inserts (C‐P4: 4.2 ± 0.4 ng/mL at 7 d; 3.6 ± 0.2 ng/mL at 14 d), although increased progesterone concentrations were found in 4/15 CON and 9/15 C‐BL cows on day 14. Bacteria were isolated from 32/61 (52%) of all uterine samples collected at the time of insertion. Uterine and vaginal swabs from CON cows showed a marked reduction in isolates over time such that 14 d after insertion only 1/15 uterine swabs grew bacteria. In contrast, C‐BL and C‐P4 treated cows failed to show reductions in the number of uterine or vaginal isolates at 14 d after device insertion. Heavy growths of Pseudomonas aeruginosa and Actinomyces pyogenes were found on the intravaginal inserts from C‐BL and C‐P4 cows. Cows enrolled in the second week of the study that received intravaginal inserts (C‐P4 + C‐BL) were more likely to have Pseudomonas isolated from the uterus than those enrolled in week 1 (1/18 versus 14/28). Conclusions The presence of two intravaginal inserts, regardless of hormone content, substantially altered the profile of uterine and vaginal bacteria in early postpartum beef cows. It was suspected that because of the early stage at insert application, the cervix had not involuted sufficiently to provide an adequate microbial barrier to the uterus. Pseudomonas aeruginosa was the predominant species isolated from both uterine and insert cultures after 14 d of treatment but may have been a contaminant, due to the greater proportion of cows infected with it that had been enrolled in the second week of the study.
The relationship between the Feline Coronavirus antibody titre and the age, breed, gender and health status of Australian cats
Authors: BELL ET , MALIK R. , NORRIS JM 
Published: June 2007

Objectives To investigate the relationship between Feline Coronavirus (FCoV) antibody titres and age, breed, gender and health status of Australian cats Design Retrospective study Procedure Results from two serological tests that measure FCoV antibody levels, the Coronase test and the 7B Feline Infectious Peritonitis (FIP) test, were recorded over a 2‐year period, with patient signalment, history, presenting complaint and the reason for ordering the test (as available). Results from each antibody test were related to four explanatory variables (breed, age, gender and health status at the time of blood collection) using univariate ordinal logistic regression analyses, Mann Whitney U tests, one‐sample sign tests or Kruskal‐Wallis analyses, as appropriate. Results Results from 637 Coronase and 191 7B FIP antibody tests were recorded. There were significant differences in median Coronase antibody titres between breeds of cats (P<0.0005). Specifically, the median Coronase antibody titres of Siamese, Persians, Domestic Shorthairs and Bengal cats (100) were significantly lower than that of British Shorthairs, Cornish Rex and Burmese cats (400, P<0.0005). There was no statistical relationship between the Coronase or 7B FIP antibody titres and age, gender or overall health status, even when considering only those cats in which clinical signs suggestive of FIP were present. Conclusion This study reinforces the complexity of interpreting serological tests for FCoV in both healthy cats and patients with signs compatible with FIP. Unique to this study is the detection of a significant relationship between breed and median FCoV antibody titre. This supports the theory that breed related differences exist in response to FCoV infection. The distribution of median Coronase antibody titres by breed was very similar to the pattern of breed predisposition to FIP recently reported in Sydney.1
Knowledge in Action
Authors: GIBBONS JOHN 
Published: May 2007

This paper argues that the role of knowledge in the explanation and production of intentional action is as indispensable as the roles of belief and desire. If we are interested in explaining intentional actions rather than intentions or attempts, we need to make reference to more than the agent's beliefs and desires. It is easy to see how the truth of your beliefs, or perhaps, facts about a setting will be involved in the explanation of an action. If you believe you can stop your car by pressing a pedal, then, if your belief is true, you will stop. If it is false, you will not. By considering cases of unintentional actions, actions involving luck and cases of deviant causal chains, I show why knowledge is required. By looking at the notion of causal relevance, I argue that the connection between knowledge and action is causal and not merely conceptual.“What knowledge adds to belief is not psychologically relevant.”1—Stephen Stich
Open Questions and the Manifest Image
Authors: KALDERON MARK ELI 
Published: May 2007

The essay argues that, on their usual metalinguistic reconstructions, the open question argument and Frege's puzzle are variants of the same argument. Each are arguments to a conclusion about a difference in meaning; each deploy compositionality as a premise; and each deploy a premise linking epistemic features of sentences with their meaning (which, given certain meaning‐platonist assumptions, can be interpreted as a universal instantiation of Leibniz's law). Given these parallels, each is sound just in case the other is. They are, in fact, unsound. The essay first argues that reformulations of these arguments directly in terms of Leibniz's law are unsound and then that subarguments of the metalinguistic versions are unsound for structurally similar reasons. Finally, given how the theory/observation distinction is deployed in linguistic practice, the meaning‐platonist assumptions are shown to be optional.Shouldn't philosophers be permitted to rise above faith in grammar? All due respect for governesses–but hasn't the time come for philosophy to renounce the faith of governesses?FRIEDRICH NIETZSCHE, Beyond Good and Evil, 1886
Aesthetic Testimony: What Can We Learn from Others about Beauty and Art?1
Authors: MESKIN AARON 
Published: May 2007

The thesis that aesthetic testimony cannot provide aesthetic justification or knowledge is widely accepted–even by realists about aesthetic properties and values. This Kantian position is mistaken. Some testimony about beauty and artistic value can provide a degree of aesthetic justification and, perhaps, even knowledge. That is, there are cases in which one can be justified in making an aesthetic judgment purely on the basis of someone else's testimony. But widespread aesthetic unreliability creates a problem for much aesthetic testimony. Hence, most testimony about art does not have much epistemic value. The situation is somewhat different with respect to aesthetic testimony about nature, proofs, and theories.And yet he realizes clearly that other people's approval in no way provides him with a valid proof by which to judge beauty; even though others may perhaps see and observe for him, and even though what many have seen the same way may serve him, who believes he saw it differently, as a sufficient basis of proof for a theoretical and hence logical judgment, yet the fact that others have liked something can never serve him as a basis for an aesthetic judgment.Immanuel Kant, Critique of Judgment
Evidence that branches of evergreen angiosperm and coniferous trees differ in hydraulic conductance but not in Huber values
Authors: Lusk Christopher H. , Jiménez-Castillo Mylthon , Salazar-Ortega Nicolás 
Published: June 2007

The hydraulic efficiency conferred by vessels is regarded as one of the key innovations explaining the historical rise of the angiosperms at the expense of the gymnosperms. Few studies, however, have compared the structure and function of xylem and their relationships with foliage traits in evergreen representatives of both groups. We measured sapwood cross-sectional area, conduit diameters, hydraulic conductance, and leaf area of fine branches (2.5–7.5 mm diameter) of five conifers and eight evergreen angiosperm trees in evergreen temperate forests in south-central Chile. Conductance of both lineages was higher at Los Lleuques, a warm temperate site with strong Mediterranean influence, than in a cool temperate rain forest at Puyehue. At a common sapwood cross-sectional area, angiosperm branches at both sites had greater hydraulic conductance (G) than conifers, but similar leaf areas. Branch conductance normalized by subtended leaf area (GL) at both sites was, therefore, higher in angiosperms than in conifers. Hydraulically weighted mean conduit diameters were much larger in angiosperms than in conifers, although this difference was less marked at Puyehue, the cooler of the two sites. Conduits of the vesselless rain forest angiosperm Drimys winteri J.R. & G. Forst were wider than those of coniferous associates, although narrower than angiosperm vessels. However, GL of D. winteri was within the range of values measured for vesselbearing angiosperms at the same site. The observed differences in xylem structure and function correlate with evidence that evergreen angiosperms have higher average stomatal conductance and photosynthetic capacity than their coniferous associates in southern temperate forests. Comparisons of conifers and angiosperm branches thus suggest that the superior capacity of angiosperm conduits is attributable to the development of higher gas-exchange rates per unit leaf area, rather than to a more extensive leaf area. Results also suggest that the tracheary elements of some vesselless angiosperms differ in width and hydraulic efficiency from conifer tracheids.
Thermogenesis in Syngonium (Araceae)
Authors: Chouteau Mathieu , Barabé Denis , Gibernau Marc 
Published: June 2007

Floral cycles and spadix temperatures were recorded for two species of Syngonium: Syngonium schottianum Wendl. ex Schott (section Cordatum) and Syngonium angustatum Schott (section Syngonium). Both species exhibited a 3-day flowering cycle, beginning with stigma receptivity and opening of the spathe the first day, the female phase continues over the second day, and the male phase continues over the third day. These species displayed two distinct patterns of heat production during flowering. In S. schottianum, the spadix warmed up twice during the beginning of the second and third nights, but in S. angustatum, the spadix warmed up twice the second day, once the second night, and once the third day. These different thermogenic cycles are discussed in regard to other genera that are phylogenetically close or sharing similar flowering and thermogenic cycles.
Inhibition of Tumor Growth and Metastasis by Depletion of Vesicular Sorting Protein Hrs: Its Regulatory Role on E-Cadherin and β-Catenin
Authors: Toyoshima Masafumi , Tanaka Nobuyuki , Aoki Jun , Tanaka Yoshinori , Murata Kazuko , Kyuuma Masanao , Kobayashi Hideyuki , Ishii Naoto , Yaegashi Nobuo , Sugamura Kazuo 
Published: June 2007

Abstract
Abnormally high signals from receptor tyrosine kinases (RTK) are associated with carcinogenesis, and impaired deactivation of RTKs may also be a mechanism in cancer. Hepatocyte growth factor–regulated tyrosine kinase substrate (Hrs) is one of the master regulators that sort activated receptors toward lysosomes and shut down their signals. Hrs contains a ubiquitin-interacting motif and is involved in the endosomal sorting of monoubiquitinated membrane proteins, such as growth factor receptor and E-cadherin. Here, we investigated the role of Hrs in determining the malignancy of cancer cells and discovered that the targeted disruption of Hrs by small interfering RNA effectively attenuated the proliferation, anchorage-independent growth, tumorigenesis, and metastatic potential of HeLa cells in vitro and in vivo. The restoration of Hrs expression increased cell proliferation and anchorage-independent growth in a mouse embryonic fibroblast line established from a Hrs knockout mouse. Further analysis revealed that Hrs depletion was associated with the up-regulation of E-cadherin and reduced β-catenin signaling. The aberrant accumulation of E-cadherin most likely resulted from impaired E-cadherin degradation in lysosomes. These results suggest that Hrs may play a critical role in determining the malignancy of cancer cells by regulating the degradation of E-cadherin. [Cancer Res 2007;67(11):5162–71]
Oncogenic Potential of the Nuclear Receptor Coregulator Proline-, Glutamic Acid–, Leucine-Rich Protein 1/Modulator of the Nongenomic Actions of the Estrogen Receptor
Authors: Rajhans Rajib , Nair Sujit , Holden Alan H. , Kumar Rakesh , Tekmal Rajeshwar Rao , Vadlamudi Ratna K. 
Published: June 2007

Abstract
Proline-, glutamic acid–, leucine-rich protein 1 (PELP1), a novel nuclear receptor coactivator, and its expression is deregulated in hormone-dependent cancers, including those of the breast, endometrium, and ovary. PELP1 interacts with estrogen receptor and modulates its genomic and nongenomic functions. In this study, we examined whether PELP1 functions as an oncogene. The overexpression of PELP1 in fibroblasts and epithelial model cells resulted in cellular transformation. PELP1 also enhanced the transformation potential of c-Src kinase in focus formation assays, and PELP1 overexpression potentiated estradiol-mediated cell migratory potential and anchorage-independent growth. Using PELP1-small interfering RNA, we provided evidence that endogenous PELP1 plays an essential role in E2-mediated anchorage-independent growth, cell migration, and cytoskeletal changes. When compared with control vector transfectants, breast cancer cells stably overexpressing PELP1 showed a rapid tumor growth in xenograft studies. Immunohistochemical analysis of PELP1 expression using a tumor progression array of 252 breast carcinomas and normal breast tissue specimens revealed that PELP1 expression is deregulated to a greater degree in higher grade node-positive invasive tumors than in normal breast tissue or ductal carcinoma in situ. Our data suggest that PELP1 is a potential oncogene, that its expression is deregulated during cancer progression, and that PELP1 may play a role in oncogenesis. [Cancer Res 2007;67(11):5505–12]
Molecular Features of Hormone-Refractory Prostate Cancer Cells by Genome-Wide Gene Expression Profiles
Authors: Tamura Kenji , Furihata Mutsuo , Tsunoda Tatsuhiko , Ashida Shingo , Takata Ryo , Obara Wataru , Yoshioka Hiroki , Daigo Yataro , Nasu Yasutomo , Kumon Hiromi , Konaka Hiroyuki , Namiki Mikio , Tozawa Keiichi , Kohri Kenjiro , Tanji Nozomu , Yokoyama Masayoshi , Shimazui Toru , Akaza Hideyuki , Mizutani Yoichi , Miki Tsuneharu , Fujioka Tomoaki , Shuin Taro , Nakamura Yusuke , Nakagawa Hidewaki 
Published: June 2007

Abstract
One of the most critical issues in prostate cancer clinic is emerging hormone-refractory prostate cancers (HRPCs) and their management. Prostate cancer is usually androgen dependent and responds well to androgen ablation therapy. However, at a certain stage, they eventually acquire androgen-independent and more aggressive phenotype and show poor response to any anticancer therapies. To characterize the molecular features of clinical HRPCs, we analyzed gene expression profiles of 25 clinical HRPCs and 10 hormone-sensitive prostate cancers (HSPCs) by genome-wide cDNA microarrays combining with laser microbeam microdissection. An unsupervised hierarchical clustering analysis clearly distinguished expression patterns of HRPC cells from those of HSPC cells. In addition, primary and metastatic HRPCs from three patients were closely clustered regardless of metastatic organs. A supervised analysis and permutation test identified 36 up-regulated genes and 70 down-regulated genes in HRPCs compared with HSPCs (average fold difference &gt; 1.5; P &lt; 0.0001). We observed overexpression of AR, ANLN, and SNRPE and down-regulation of NR4A1, CYP27A1, and HLA-A antigen in HRPC progression. AR overexpression is likely to play a central role of hormone-refractory phenotype, and other genes we identified were considered to be related to more aggressive phenotype of clinical HRPCs, and in fact, knockdown of these overexpressing genes by small interfering RNA resulted in drastic attenuation of prostate cancer cell viability. Our microarray analysis of HRPC cells should provide useful information to understand the molecular mechanism of HRPC progression and to identify molecular targets for development of HRPC treatment. [Cancer Res 2007;67(11):5117–25]
Proton Pump Inhibitors Induce Apoptosis of Human B-Cell Tumors through a Caspase-Independent Mechanism Involving Reactive Oxygen Species
Authors: De Milito Angelo , Iessi Elisabetta , Logozzi Mariantonia , Lozupone Francesco , Spada Massimo , Marino Maria Lucia , Federici Cristina , Perdicchio Maurizio , Matarrese Paola , Lugini Luana , Nilsson Anna , Fais Stefano 
Published: June 2007

Abstract
Proton pumps like the vacuolar-type H+ ATPase (V-ATPase) are involved in the control of cellular pH in normal and tumor cells. Treatment with proton pump inhibitors (PPI) induces sensitization of cancer cells to chemotherapeutics via modifications of cellular pH gradients. It is also known that low pH is the most suitable condition for a full PPI activation. Here, we tested whether PPI treatment in unbuffered culture conditions could affect survival and proliferation of human B-cell tumors. First, we showed that PPI treatment increased the sensitivity to vinblastine of a pre-B acute lymphoblastic leukemia (ALL) cell line. PPI, per se, induced a dose-dependent inhibition of proliferation of tumor B cells, which was associated with a dose- and time-dependent apoptotic-like cytotoxicity in B-cell lines and leukemic cells from patients with pre-B ALL. The effect of PPI was mediated by a very early production of reactive oxygen species (ROS), that preceded alkalinization of lysosomal pH, lysosomal membrane permeabilization, and cytosol acidification, suggesting an early destabilization of the acidic vesicular compartment. Lysosomal alterations were followed by mitochondrial membrane depolarization, release of cytochrome c, chromatin condensation, and caspase activation. However, inhibition of caspase activity did not affect PPI-induced cell death, whereas specific inhibition of ROS by an antioxidant (N-acetylcysteine) significantly delayed cell death and protected both lysosomal and mitochondrial membranes. The proapoptotic activity of PPI was consistent with a clear inhibition of tumor growth following PPI treatment of B-cell lymphoma in severe combined immunodeficient mice. This study further supports the importance of acidity and pH gradients in tumor cell homeostasis and suggests new therapeutic approaches for human B-cell tumors based on PPI. [Cancer Res 2007;67(11):5408–17]
MUC1 Oncoprotein Promotes Refractoriness to Chemotherapy in Thyroid Cancer Cells
Authors: Siragusa Mauro , Zerilli Monica , Iovino Flora , Francipane Maria Giovanna , Lombardo Ylenia , Ricci-Vitiani Lucia , Di Gesù Giuseppe , Todaro Matilde , De Maria Ruggero , Stassi Giorgio 
Published: June 2007

Abstract
Overexpression of MUC1 oncoprotein is frequently observed in cancer and contributes to confer resistance to genotoxic agents. Papillary, follicular, and anaplastic thyroid carcinomas are the three forms of thyroid epithelial cancer. Anaplastic tumors are less differentiated and extremely aggressive, characterized by a poor prognosis. Little is known about the role of MUC1 in thyroid cancer. We recently showed that autocrine production of interleukin (IL)-4 and IL-10 controls thyroid cancer cell survival, growth, and resistance to chemotherapy through activation of Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) and phosphatidylinositide 3′-OH kinase (PI3K)/Akt pathways. In the present study, we showed that MUC1 COOH-terminal subunit (MUC1-C) is overexpressed in all the histologic variants of thyroid cancer cells and localizes to mitochondria where it interferes with the release of mitochondrial proapoptotic proteins. Moreover, IL-4 and IL-10 promote the increase of MUC1-C expression levels in normal thyroid cells, whereas blockage of both cytokines or neutralization of JAK/STAT and PI3K/Akt pathways through the exogenous expression of SOCS-1 and AktK179M leads to a significant decrease of MUC1-C in primary thyroid cancer cells. Interestingly, down-regulation of MUC1 expression by direct targeting with RNA interference sensitizes anaplastic thyroid cancer cells to chemotherapy-induced apoptosis in vitro. Thus, MUC1 is a main component of the survival network acting in thyroid cancer and could be considered a key molecular target for sensitizing cancer cells to conventional or novel treatments. [Cancer Res 2007;67(11):5522–30]
Noxa Up-regulation and Mcl-1 Cleavage Are Associated to Apoptosis Induction by Bortezomib in Multiple Myeloma
Authors: Gomez-Bougie Patricia , Wuillème-Toumi Soraya , Ménoret Emmanuelle , Trichet Valérie , Robillard Nelly , Philippe Moreau , Bataille Régis , Amiot Martine 
Published: June 2007

Abstract
Targeting the ubiquitin-proteasome pathway has emerged as a potent anticancer strategy. Bortezomib, a specific proteasome inhibitor, has been approved for the treatment of relapsed or refractory multiple myeloma. Multiple myeloma cell survival is highly dependent on Mcl-1 antiapoptotic molecules. In a recent study, proteasome inhibitors induced Mcl-1 accumulation that slowed down their proapoptotic effects. Consequently, we investigated the role of Bcl-2 family members in bortezomib-induced apoptosis. We found that bortezomib induced apoptosis in five of seven human myeloma cell lines (HMCL). Bortezomib-induced apoptosis was associated with Mcl-1 cleavage regardless of Mcl-1L accumulation. Furthermore, RNA interference mediated Mcl-1 decrease and sensitized RPMI-8226 HMCL to bortezomib, highlighting the contribution of Mcl-1 in bortezomib-induced apoptosis. Interestingly, an important induction of Noxa was found in all sensitive HMCL both at protein and mRNA level. Concomitant to Mcl-1 cleavage and Noxa induction, we also found caspase-3, caspase-8, and caspase-9 activation. Under bortezomib treatment, Mcl-1L/Noxa complexes were highly increased, Mcl-1/Bak complexes were disrupted, and there was an accumulation of free Noxa. Finally, we observed a dissociation of Mcl-1/Bim complexes that may be due to a displacement of Bim induced by Noxa. Thus, in myeloma cells, the mechanistic basis for bortezomib sensitivity can be explained mainly by the model in which the sensitizer Noxa can displace Bim, a BH3-only activator, from Mcl-1, thus leading to Bax/Bak activation. [Cancer Res 2007;67(11):5418–24]