bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploratory pedagogical research of a bespoke eye movement desensitisation and reprocessing therapy training for midwives
Authors: McCullough Julie Elizabeth May , Gillen Patricia , Miller Paul William , Sinclair Marlene , Black Rachel Jane , Miller Paula Taylor , Farrell Derek Patrick 
Published: April 2024

AbstractEye movement desensitisation and reprocessing (EMDR) therapy is a transdiagnostic, comprehensive, integrative, evidence‐based treatment intervention for post‐traumatic stress disorder (PTSD), complex PTSD, and perinatal PTSD. PTSD can arise from an experience of pregnancy or birth related trauma. Despite this, there is limited availability and access to EMDR therapy within the United Kingdom National Health Service. EMDR is a psychotherapeutic intervention which is usually delivered by highly specialist mental health professionals. However, with such a robust protocol, it is appropriate to consider if other health professionals should be trained to deliver EMDR. Humanitarian trauma capacity‐building projects in a global context have shown that task shifting can assist with addressing unmet mental health therapy needs. Midwives are highly skilled graduates working in the perinatal period who understand that women's emotional health is as important as their physical health. Therefore, it was proposed that EMDR knowledge and skills could be efficiently task shifted to midwives. The aim and objectives were to train midwives to deliver modified EMDR scripted protocols and techniques and explore qualitative and quantitative outcomes of a bespoke EMDR for midwives (EMDR‐m) educational programme. The online training was delivered to the midwives over 4 days with clinical practicums incorporated throughout. Pre and post‐tests demonstrated an increase in their EMDR knowledge, skills and confidence. EMDR Group Supervision provided by three experienced EMDR Accredited Practitioners was mandatory for 6 weeks post‐training and ongoing one‐to‐one supervision was made available. Midwives scored the course 9.6/10 (range 8–10) and described it as ‘amazing’ and ‘invaluable’. Challenges for the future include ring‐fenced time and an appropriate space to deliver the therapy. Those midwives who completed the training have progressed to deliver early EMDR‐m interventions in a perinatal mental health research study in their own Health and Social Care Trust (reported elsewhere).
Identifying gray matter alterations in Cushing's disease using machine learning: An interpretable approach
Authors: Long Yue , Ren Jie , Cheng FuChao , Duan YuMei , Wang BaoFeng , Sun Yuhao , Sun QingFang , Bian LiuGuan , Yi JunChen , Qin Ying , Huang RongBing , Guo WeiTong , Jiang Hong , Liu Chang , Feng Xiao , Qin Ling 
Published: April 2024

AbstractBackgroundCushing's Disease (CD) is a rare clinical syndrome characterized by excessive secretion of adrenocorticotrophic hormone, leading to significant functional and structural brain alterations as observed in Magnetic Resonance Imaging (MRI). While traditional statistical analysis has been widely employed to investigate these MRI changes in CD, it has lacked the ability to predict individual‐level outcomes.PurposeTo address this problem, this paper has proposed an interpretable machine learning (ML) framework, including model‐level assessment, feature‐level assessment, and biology‐level assessment to ensure a comprehensive analysis based on structural MRI of CD.MethodsThe ML framework has effectively identified the changes in brain regions in the stage of model‐level assessment, verified the effectiveness of these altered brain regions to predict CD from normal controls in the stage of feature‐level assessment, and carried out a correlation analysis between altered brain regions and clinical symptoms in the stage of biology‐level assessment.ResultsThe experimental results of this study have demonstrated that the Insula, Fusiform gyrus, Superior frontal gyrus, Precuneus, and the opercular portion of the Inferior frontal gyrus of CD showed significant alterations in brain regions. Furthermore, our study has revealed significant correlations between clinical symptoms and the frontotemporal lobes, insulin, and olfactory cortex, which also have been confirmed by previous studies.ConclusionsThe ML framework proposed in this study exhibits exceptional potential in uncovering the intricate pathophysiological mechanisms underlying CD, with potential applicability in diagnosing other diseases.
Pseudoexon activation by deep intronic variation in GNE myopathy with thrombocytopenia
Authors: Jiao Kexin , Cheng Nachuan , Huan Xiao , Zhang Jialong , Ding Yu , Luan Xinghua , Liu LingChun , Wang Xilu , Zhu Bochen , Du Kunzhao , Fan Jiale , Gao Mingshi , Xia Xingyu , Wang Ningning , Wang Tao , Xi Jianying , Luo Sushan , Lu Jiahong , Zhao Chongbo , Yue Dongyue , Zhu Wenhua 
Published: April 2024

AbstractIntroduction/AimsGNE myopathy is a rare autosomal recessive disorder caused by pathogenic variants in the GNE gene, which is essential for the sialic acid biosynthesis pathway. Although over 300 GNE variants have been reported, some patients remain undiagnosed with monoallelic pathogenic variants. This study aims to analyze the entire GNE genomic region to identify novel pathogenic variants.MethodsPatients with clinically compatible GNE myopathy and monoallelic pathogenic variants in the GNE gene were enrolled. The other GNE pathogenic variant was verified using comprehensive methods including exon 2 quantitative polymerase chain reaction and nanopore long‐read single‐molecule sequencing (LRS).ResultsA deep intronic GNE variant, c.862+870C>T, was identified in nine patients from eight unrelated families. This variant generates a cryptic splice site, resulting in the activation of a novel pseudoexon between exons 5 and 6. It results in the insertion of an extra 146 nucleotides into the messengerRNA (mRNA), which is predicted to result in a truncated humanGNE1(hGNE1) protein. Peanut agglutinin（PNA） lectin staining of muscle tissues showed reduced sialylation of mucin O‐glycans on sarcolemmal glycoproteins. Notably, a third of patients with the c.862+870C>T variant exhibited thrombocytopenia. A common core haplotype harboring the deep intronic GNE variant was found in all these patients.DiscussionThe transcript with pseudoexon activation potentially affects sialic acid biosynthesis via nonsense‐mediated mRNA decay, or resulting in a truncated hGNE1 protein, which interferes with normal enzyme function. LRS is expected to be more frequently incorporated in genetic analysis given its efficacy in detecting hard‐to‐find pathogenic variants.
Sport fish movement responses to a littoral zone coarse woody habitat addition
Authors: Smith Quinnlan C. , Shaw Stephanie L. , Raabe Joshua K. , Sass Greg G. , Hrabik Thomas R. 
Published: April 2024

Abstract

Objective
Fish movements and distributions throughout an ecosystem are important for understanding ecological interactions, conservation, and management. Coarse woody habitat (CWH) additions are a popular habitat enhancement tool that can influence movements and distributions of fishes; however, behavioral responses in relation to CWH additions are understudied. The objectives of our study were to test for changes in habitat use and behavioral responses of Muskellunge Esox masquinongy, Smallmouth Bass Micropterus dolomieu, and Walleye Sander vitreus to CWH additions in a small inland northern lake over 3 years and to evaluate two common forms of fish tracking to estimate daily movement of these fishes.

Methods
We used passive integrated transponder tag data collected continuously at 10 littoral sites and radiotelemetry data collected biweekly during the summer–fall months prior (2017) and following (2018–2019) CWH addition to examine movement behaviors and estimate daily probability of movement for the three species among lake habitats using multistate models.

Result
Walleye were the most likely to remain near CWH, followed by Smallmouth Bass and Muskellunge. All species were more likely to move away from the modified and unmodified littoral zones and were more likely to remain in the offshore zone. Increased movement of fish may be in response to CWH creating a refuge for forage fish and subsequent increases in prey search times.

Conclusion
Our results suggest that CWH additions may alter established species-specific annual movements, distributions, and habitat use to differing degrees in ecosystems, as our results differ from other CWH studies. Methodologically, radio-tagging was most effective for offshore tracking but most labor intensive. Novel passive integrated transponder arrays (less labor intensive once installed) worked well to monitor movement between habitat types in shallow littoral areas. Long-term monitoring of behavioral changes will be essential to assessing the stability changes caused by these habitat alterations.
Stochastic assessment of 3‐D tunnels in near‐fault ground motion using modified domain reduction method
Authors: Banjare Bhavesh , Avatar Gauri Ranjan Krishna Chand 
Published: April 2024

AbstractA robust assessment of tunnels due to uncertainties present in soil and ground motion properties can affect the dynamic response of these structures. In this paper, a stochastic analysis considering an aleatory variability in shear velocity Vs by performing Monte Carlo simulations and assessing its influence on underground tunnels. To numerically assess the response of the soil‐tunnel system to near‐fault earthquake ground motion, the required computational domain usually spans multiple kilometers including both earthquake fault and the structure. Thus, these simulations are computationally expensive. An efficient alternative method, that is, the Domain Reduction Method (DRM), in which a modular two‐step methodology for reducing the computational costs in the large domain analysis is employed. A 3‐D soil‐tunnel structure interaction is modeled to simulate the response of tunnel subjected to an inclined earthquake fault. The numerical simulation of the soil‐tunnel‐fault system is conducted in an open‐source FE package called Multi‐hazard Analysis for STOchastic time DOmaiN phenomena (MASTODON) based on MOOSE framework. The interactive analyses are carried out for three distinct types of soils based on National Earthquake Hazards Reduction Program (NEHRP) provision, that is, soft, medium, and hard, by generating 130 realizations. The results provide many insights into the influence of local site effects, that is, frequency shift for the peak response and the importance of input ground motion characteristics that govern the response of underground structures. The dynamic response of the structure is very sensitive to the uncertainty in rock material properties, especially, the stiffness of the rock sample and the shear wave velocity.
Post mortem analysis of hepatic volume and lipid content by magnetic resonance imaging and spectroscopy in fixed murine neonates
Authors: Jonuscheit Marc , Uhlemeyer Celina , Korzekwa Benedict , Schouwink Marten , Öner‐Sieben Soner , Ensenauer Regina , Roden Michael , Belgardt Bengt‐Frederik , Schrauwen‐Hinderling Vera B. 
Published: April 2024

AbstractMaternal obesity and hyperglycemia are linked to an elevated risk for obesity, diabetes, and steatotic liver disease in the adult offspring. To establish and validate a noninvasive workflow for perinatal metabolic phenotyping, fixed neonates of common mouse strains were analyzed postmortem via magnetic resonance imaging (MRI)/magnetic resonance spectroscopy (MRS) to assess liver volume and hepatic lipid (HL) content. The key advantage of nondestructive MRI/MRS analysis is the possibility of further tissue analyses, such as immunohistochemistry, RNA extraction, and even proteomics, maximizing the data that can be gained per individual and therefore facilitating comprehensive correlation analyses. This study employed an MRI and 1H‐MRS workflow to measure liver volume and HL content in 65 paraformaldehyde‐fixed murine neonates at 11.7 T. Liver volume was obtained using semiautomatic segmentation of MRI acquired by a RARE sequence with 0.5‐mm slice thickness. HL content was measured by a STEAM sequence, applied with and without water suppression. T1 and T2 relaxation times of lipids and water were measured for respective correction of signal intensity. The HL content, given as CH2/(CH2 + H2O), was calculated, and the intrasession repeatability of the method was tested. The established workflow yielded robust results with a variation of ~3% in repeated measurements for HL content determination. HL content measurements were further validated by correlation analysis with biochemically assessed triglyceride contents (R2 = 0.795) that were measured in littermates. In addition, image quality also allowed quantification of subcutaneous adipose tissue and stomach diameter. The highest HL content was measured in C57Bl/6N (4.2%) and the largest liver volume and stomach diameter in CBA (53.1 mm3 and 6.73 mm) and NMRI (51.4 mm3 and 5.96 mm) neonates, which also had the most subcutaneous adipose tissue. The observed effects were independent of sex and litter size. In conclusion, we have successfully tested and validated a robust MRI/MRS workflow that allows assessment of morphology and HL content and further enables paraformaldehyde‐fixed tissue‐compatible subsequent analyses in murine neonates.
Availability of individual proteins for quantitative analysis in postmortem brains preserved in two different brain banks
Authors: Nagaoka Atsuko , Hino Mizuki , Izumi Ryuta , Shishido Risa , Ishibashi Miki , Hatano Masataka , Sainouchi Makoto , Kakita Akiyoshi , Tomita Hiroaki , Kunii Yasuto 
Published: April 2024

AbstractAimPostmortem brain research is necessary for elucidating the pathology of schizophrenia; an increasing number of studies require a combination of suitable tissue samples preserved at multiple brain banks. In this study, we examined whether a comparative study of protein expression levels can be conducted using postmortem brain samples preserved in different facilities.MethodsWe compared the demographic factors of postmortem brain samples preserved in two institutions and measured and compared the expression levels of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and glial fibrillary acidic protein (GFAP) in the prefrontal cortex and superior temporal gyrus. GAPDH is generally used as a loading control for western blotting, and GFAP is considered as an astrocyte marker in the brain.ResultsWe found significant differences between the two institutions in postmortem interval, age at death, and preservation time. To reduce the effects of these differences on our measurements, the parameters were set as covariates in our analyses of covariance. Subsequently, no differences in GAPDH and GFAP expression were found between institutions.ConclusionsWhen studies are conducted using brain samples preserved in different brain banks, differences in demographic factors should be carefully considered and taken into account by statistical methods to minimize their impact as much as possible. Since there was no significant difference in the protein expression levels of GAPDH and GFAP in either region between the two institutions that preserved the postmortem brains, we concluded that it is possible to perform protein quantitative analysis assuming that there is no effect of difference between two institutions.
Characterizing shallow fault zones by integrating profile, borehole and array measurements of seismic data and distributed acoustic sensing
Authors: Rein Nikolaus , Isken Marius P. , Domigall Dorina , Ohrnberger Matthias , Hannemann Katrin , Krüger Frank , Korn Michael , Dahm Torsten 
Published: April 2024

AbstractWithin the framework of the Intercontinental Scientific Drilling Programme (ICDP) ‘Drilling the Eger Rift’ project, five boreholes were drilled in the Vogtland (Germany) and West Bohemia (Czech Republic) regions. Three of them will be used to install high‐frequency three‐dimensional (3D) seismic arrays. The pilot 3D array is located 1.5 km south of Landwüst (Vogtland). The borehole, with a depth of 402 m, was equipped with eight geophones and a fibre optic cable behind the casing used for distributed acoustic sensing (DAS) measurements. The borehole is surrounded by a surface array consisting of 12 seismic stations with an aperture of 400 m. During drilling, a highly fractured zone was detected between 90 m and 165 m depth and interpreted as a possible fault zone. To characterize the fault zone, two vertical seismic profiling (VSP) experiments with drop weight sources at the surface were conducted. The aim of the VSP experiments was to estimate a local 3D seismic velocity tomography including the imaging of the steep fault zone. Our 3D tomography indicates P‐wave velocities between 1500 m/s and 3000 m/s at shallow depths (0–20 m) and higher P‐wave velocities of up to 5000 m/s at greater depths. In addition, the results suggest a NW–SE striking low‐velocity zone (LVZ; characterized by = 1500–3000 m/s), which crosses the borehole at a depth of about 90–165 m. This LVZ is inferred to be a shallow non‐tectonic, steep fault zone with a dip angle of about . The depth and width of the fault zone are supported by logging data as electrical conductivity, core recovery and changes in lithology. In this study, we present an example to test and verify 3D tomography and imaging approaches of shallow non‐tectonic fault zones based on active seismic experiments using simple surface drop weights as sources and borehole chains as well as borehole DAS behind casing as sensors, complemented by seismic stand‐alone surface arrays.
Combing through museum collections. A “museomic” application of ZooMS
Authors: Monticone Alessia , Panero Elisa , Heritier Erika , Pergolizzi Barbara , Bello Federica Dal , Mecarelli Enrica , Boano Rosa , de Vingo Paolo , Codlin Maria , Pessione Enrica , Demarchi Beatrice 
Published: April 2024

AbstractThis article presents the application of Zooarchaeology by Mass Spectrometry (ZooMS) to osseous Longobard artifacts from the collection of the “Musei Reali di Torino” (MRT; Torino, Italy). Like most archaeological items made of worked bone/antler in museum collections, the raw material of such specimens is usually attributed to deer, often without accurate taxonomic attribution. Therefore, the main aim of the present investigation was to shed light on taxonomical aspects using biomolecular approaches.We first examined the collagen preservation of the artifacts, then we compared three sampling methods (invasive, eraser‐based, and bag‐based), and we evaluated the quality of the collagen fingerprint obtained. Overall, we found a good, albeit not optimal, biomolecular preservation status, even in heavily restored objects coming from the 19th‐century collections.Out of 37 specimens analyzed through matrix‐assisted laser desorption ionization–time of flight–mass spectrometry (MALDI‐TOF‐MS) and nanoHPLC‐tandem MS, 31 yielded usable data. The results confirmed the widespread use of cervid as the osseous raw material for comb‐making in Longobard times in Piedmont, but we also found that bovine bones (Bos but also other taxa belonging to family Bovidae, such as caprines) and equid bones were exploited—demonstrating opportunistic use of animal resources. As far as the method is concerned, the ZooMS peptide markers useful to distinguish between bovids and cervids (m/z 1580 vsm/z 1550) (Buckley et al., 2009) are more frequently detected when analyzing bone chips, that is, with the invasive sampling method, rather than collagen extracts obtained using non‐invasive techniques. Nonetheless, the eraser method (eZooMS) seems to be a good trade‐off between invasivity and quality of the information obtained: eZooMS sampling does not leave visible marks on the object and therefore can contribute to facilitating the routine application of biomolecular methods in the daily practice of museum conservation laboratories. Indeed, an important outcome of the present study has been the establishment of a close collaboration between museum and biomolecular specialists.Taken together, our results suggest that the Longobards had a preference towards locally available resources, although this work did not highlight a clear association between raw material (deer, cattle, and other bovids) and object typology (in the case of combs) or function, except for buttons. The overall information obtained by this study confirms the potential of biomolecular approaches for reconstructing the biography of museum objects with a long and complex life and demonstrates the value of zooarchaeological study of museum collections.
Repeated photodynamic therapy using a chlorin e6‐embedded device to prolong the therapeutic effects on obesity
Authors: Park Jung‐Hoon , Kim Ji Won , Ryu Dae Sung , Lee Hyeonseung , Na Hee Kyong , Noh Jin Hee , Kim Do Hoon , Lee Sanghee , Na Kun , Jung Hwoon‐Yong 
Published: April 2024

AbstractObjectiveThis study aimed to investigate the efficacy and safety of repeated photodynamic therapy (PDT) with a chlorin e6 (Ce6)‐embedded intragastric satiety‐inducing device (ISD) to maintain therapeutic effects of obesity in a juvenile pig.MethodsThe Ce6‐embedded ISD was fabricated with a dipping method. Twelve pigs were divided into four groups of three and were administered control, single, biweekly, or weekly PDT, respectively. The therapeutic effects were assessed by comparing the results of phototoxicity, endoscopy, fluoroscopy, hormone and weight changes, and histological examination.ResultsThe percentage of total body weight gain was significantly suppressed in PDT‐treated pigs compared with control pigs (all p < 0.001). This suppression persisted in the repeated PDT groups, but percentage of total body weight gain gradually increased when PDT was stopped. Ghrelin levels in the PDT‐treated groups were significantly lower and leptin levels were significantly higher than those in the control group (all p < 0.05). Inflammatory cell infiltration, collagen, TUNEL, and anti‐ghrelin‐positive deposition in the weekly group were significantly higher than those in the control, single, and biweekly groups (all p < 0.01).ConclusionsRepeated and periodic PDT was technically feasible and safe and successfully maintained the therapeutic effects against obesity while eliminating the indwelling time and reducing ISD‐related complications in pigs.