bluuebunny/crossref_metadata_2025_split huggingface dataset:
Indoor residual spraying uptake and its effect on malaria morbidity in Ngoma district, Eastern province of Rwanda, 2018-2021
Authors: Nsekuye Olivier , Malamba Samuel S. , El-Khatib Ziad , Omolo Jared , Mangara Jean-Louis N. , Munyakanage Dunia , Uwimana Aline , Umutoni Angela , Lucchi Naomi W. , Rwagasore Edson , Ntabanganyimana Daniel , Niyoyita Jean-Claude , Uwayo Henri Desire , Ntakirutimana Theoneste , Rwunganira Samuel 
Published: March 2024

Abstract

Background
Indoor residual spraying (IRS) was implemented in Rwanda among districts with high transmission of malaria, including Ngoma District, which received the first IRS (IRS-1) in March 2019 before the peak season, and the second IRS (IRS-2) campaign in August 2020, which targeted 89,331 structures. This study was conducted to assess factors associated with IRS uptake and the effect of IRS intervention on malaria morbidity in the Ngoma district, Eastern Province, Rwanda.
Method
A household survey using a multistage cluster sampling design was used to randomly select households. A well-structured questionnaire was administered to the head of household or the designated household in May 2021. A logistic regression model adjusted for the complex survey design and weighted to account for the sampling design was used to assess factors associated with IRS uptake. A secondary data analysis of malaria cases registered in the Rwanda Health Management Information System (RHMIS) from January 2015 to December 2022 in Ngoma district was used to determine the effect of IRS on malaria morbidity using interrupted time series analysis.
Results
Six hundred thirty-six households headed by self-employed (aOR = 0.07; 95% CI: 0.01–0.55) and unemployed (aOR = 0.18; 95% CI: 0.03–0.99) individuals were less likely to take up IRS than households headed by farmers. Households receiving information about the IRS via media (aOR = 0.01; 95% CI: 0.00-0.17) were less likely to use the IRS than were those receiving information via community health workers. From the RHMIS, 919,843 malaria cases were identified. In the interrupted time series analysis, the baseline number of adjusted malaria cases was approximately 16,920. The first intervention in March 2019 led to a significant reduction of approximately 14,380 patients (p < 0.001), while the second intervention in August 2020 led to a decrease of approximately 2,495 patients, although the difference was not statistically significant (p = 0.098).
Conclusion
This study underscores the ability of IRS to reduce malaria incidence in Ngoma District. The influence of socioeconomic factors and information sources on IRS uptake calls for targeted strategies, comprehensive IRS education, and an integrated malaria control approach, including bed net usage and other prevention and control interventions.
A new synthetic route towards the formation of rapid crystal of dinuclear dysprosium (III) complex using ligand derived from o-vanillin: syntheses, crystal structure and magnetic properties
Authors: Saha Samarendra Nath , Halder Pabitra , Mandal Soumik 
Published: March 2024

Abstract
A new dinuclear dysprosium (III) complex has been synthesized with the formula [DyIII2(valpico)2(NO3)4].4CH3CN, where (Hvalpico = 2-methoxy-6-((pyridin-2-ylmethylimino)methyl)phenol) in acetonitrile medium. The single crystals started to form from acetonitrile solution after 5 minutes of reaction and completed within 10 minutes at room temperature. The crystals were of good quality for single crystal X-ray diffraction measurement. The structural and magnetic properties of the dinuclear (Dy2) phenoxo-bridged motif are explored. The magnetic property of the Dy-dimer complex show single molecular magnetic (SMM) behaviour.
Multifunctional alkaline phosphatases drive the phosphorus cycle in the ocean
Authors: Saavedra Daniel E. M. , González José M. , Klaushofer Katharina , Afjehi-Sadat Leila , Patrick Wayne M. , Baltar Federico 
Published: March 2024

Abstract
Background
Phosphorus plays a crucial role in limiting marine ecosystems productivity. To overcome this limitation, organisms use alkaline phosphatases (APases) to obtain phosphate from organic compounds. Although traditionally viewed as isoenzymes with specific roles, recent research has uncovered the multifunctionality of PhoA, a specific APase family. However, the prevalence of enzyme multifunctionality across all APase families remains unexplored, as does the determination of the dominant APases and the microbial taxa expressing them in the oceanic water column.
Results
Our findings revealed that all major APase families are actively expressed in the global ocean and are dominated by Alteromonas, particularly in the deep ocean. Enzymatic assays with purified recombinant APases from Alteromonas mediterranea revealed multifunctional activities with substrate affinities indicating distinct ecological roles. Proteomic analysis under phosphorus limitation unveiled specific protein regulation in Alteromonas mediterranea. Investigation into the secretion signal-peptides of APase enzymes revealed that PhoA and PafA predominantly utilize the Sec pathway for secretion, whereas PhoD and PhoX are primarily transported through the Tat pathway.
Conclusions
Our findings underscore the relevance of multifunctional APases in the global ocean, illustrating how microbes utilize multifunctional alkaline phosphatase families to acquire phosphorus, while ultimately highlighting the role of multifunctional enzymes in marine biogeochemical cycles.
Promotion effect suggested by hexadecanoic acid on the oxidative tolerance of S. cerevisiae during its co-culture with E. coli
Authors: Hou Shuxin , Wang Shihui , Zheng Caijuan , Zhou Yu , Yu Changyuan , Li Hao 
Published: March 2024

Abstract
Co-fermentation performed by Saccharomyces cerevisiae and Escherichia coli or other microbes has been widely used in industrial fermentation. Meanwhile, the co-cultured microbes might regulate each other’s metabolisms or cell behaviors including oxidative tolerance through secreting molecules. Here, results based on the co-culture system of S. cerevisiae and E. coli suggested the promoting effect of E. coli on the oxidative tolerance of S. cerevisiae cells. The co-cultured E. coli could enhance S. cerevisiae cell viability through improving its membrane stability and reducing the oxidized lipid level. Meanwhile, promoting effect of the co-cultured supernatant on the oxidative tolerance of S. cerevisiae illustrated by the supernatant substitution strategy suggested that secreted compounds contained in the co-cultured supernatant contributed to the higher oxidative tolerance of S. cerevisiae. The potential key regulatory metabolite (i.e., hexadecanoic acid) with high content difference between co-cultured supernatant and the pure-cultured S. cerevisiae supernatant was discovered by GC-MS-based metabolomics strategy. And exogenous addition of hexadecanoic acid did suggest its contribution to higher oxidative tolerance of S. cerevisiae. Results presented here would contribute to the understanding of the microbial interactions and provide the foundation for improving the efficiency of co-fermentation performed by S. cerevisiae and E. coli.
RETRACTED: Influence of Ion Mobility and Electrode Structure Effects on Supercapacitors with CaO, MgO and SiO2
Authors: Yargı Önder , Bostancı Eda , Filiz Bilge Coşkuner , Kantürk Aysel Figen 
Published: March 2024

Abstract
The authors have requested that this preprint be removed from Research Square.
Role of Telephysical Therapy in Quality of Life and Satisfaction Among Patients With Musculoskeletal Disorders.
Authors: Arwa Munaza , Chaikumarn Montakarn 
Published: March 2024

Abstract
Background:
The efficacy of telephysical therapy has been tailored in several previous researches. However, its role in improving the quality of life and satisfaction of musculoskeletal disorder patients is ambiguous. The study aimed to determine the role of telephysical therapy in quality of life and satisfaction among patients with musculoskeletal disorders.
Method:
A pre-post study was conducted recruiting 327 musculoskeletal disorder patients through the platform of MARHAM. The participants were requested to fill telehealth usability questionnaire (TUQ) and the European quality of life-5-dimension questionnaire (EQ-5D) before the start of tele sessions and at the end via email and WhatsApp. All the participants answered 10 open-ended questions related to their experience and perception of telephysical therapy at the end of the intervention. The participant was excluded if he/she had any cognitive impairment, any serious medical/psychological comorbidity, or was unable to commit to the study protocol.
Results:
The majority of the participants belonged to Lahore with a mean age of 41.80 (+14.84). Paired sample t-tests showed significant differences between pre and post-values of TUQ and EQ-5D scores. Overall health score was also improved after tele sessions. Multiple regression analysis showed 35.4% of variance in satisfaction and 35% in quality of life. Age, gender, and number of sessions were the most influencing independent variables. 85.33% of participants were highly satisfied and 83% had excellent experience. They suggested in-person initial assessment and follow-up sessions.
Conclusion:
Telephysical therapy had a positive role in improving the quality of life and satisfaction among patients with musculoskeletal disorders.
Influence of Psychometric and Sleep Quality Features on Painful Mechanical Sensitivity and Pain Modulation in Patients with Temporomandibular Disorders
Authors: Salbego Rafaela Stocker , Conti Paulo César Rodrigues , Soares Flávia Fonseca Carvalho , Ferreira Dyna Mara Araújo Oliveira , Herreira-Ferreira Matheus , Netto Beatriz Amaral Lima , Svensson Peter , Costa Yuri Martins , Bonjardim Leonardo Rigoldi 
Published: March 2024

Abstract

Investigating the collective impact of psychometric properties and sleep quality on pain sensitivity in temporomandibular disorder (TMD) patients could improve clinical management strategies.
Objective: Assessing whether combined psychometric properties and sleep quality impact painful mechanical sensitivity and pain modulation in TMD patients.
Materials and Methods: A cross-sectional study using secondary data analysis of 77 TMD patients and 101 controls. All participants completed questionnaires characterizing their psychometric profile (anxiety, depression, stress and catastrophizing) and sleep quality, alongside quantitative tests for painful mechanical sensory (mechanical pain threshold (MPT), pressure pain threshold (PPT), and wind-up ratio (WUR)) and conditioned pain modulation (CPM). Participants were grouped into "High distress" or "Low distress" categories based on psychometric properties and sleep quality using hierarchical cluster and k-means analyses. Multiple linear regression evaluated the influence of TMD, age, and the distress cluster on MPT, WUR, PPT, and CPM in masseter and thenar muscles. Differences were statistically significant when p<0.05.
Results: The presence of TMD was the strongest predictor of mechanical painful sensitivity in the trigeminal region (MPT[F(3,174)=51.902;p<,001;adjusted R2=,463]; TMD presence (β=-,682) / PPT[F(3,174)=15.573;p<,001;adjusted R2=,198] TMD presence (β=-,452), and extra-trigeminal (MPT[F(3,174)=35.897;p<,001;adjusted R2=,382] TMD (β=-,647) / CPM [F(3,174)=4.106;p<,05;adjusted R2=,050] TMD presence (β=,197).
Conclusions: There is not a significant influence of impairment clusters based on psychological variables and sleep quality on painful mechanical sensitivity and pain modulation, regardless of the presence of TMD.
Clinical relevance: This outcome suggests that psychosocial factors and sleep quality may not play a decisive role in the sensory-discriminative aspect of pain, particularly concerning painful TMD.
Screening impacts of Tilmicosin induced-hepatic and renal toxicity in rats: Protection by Rhodiola Rosea extract through the involvement of oxidative stress, antioxidants, and inflammatory cytokines biomarkers
Authors: Elgendy Salwa A. , Soliman Mohamed Mohamed , Shukry Mustafa , Mohammed Lina Abdelhady , Nasr Hend Elsayed , Althobaiti Saad , Almalki Daklallah A. , Alotaibi Khalid S. , Elnoury Heba A. 
Published: March 2024

Abstract

Tilmicosin (TIL) is a semisynthetic macrolide antibiotic with a broad spectrum of activity derived from tylosin. TIL is effective in the treatment of bovine and ovine respiratory diseases caused by different microbes. In parallel, Rhodiola rosea (RHO) is a popular herbal remedy because of its anti-inflammatory and antioxidant qualities. Rats received saline or RHO for sequential 12 days. TIL was injected as a single dose subcutaneously (75 mg/kg BW) on day 6 of experiment. The protective group received RHO daily for sequential 12 days, TIL was injected as a single dose 1 hour after RHO administration on day 6 of experiment and continued for extra 6 successive days with RHO only. Samples and blood were collected for serum analysis, gene expression and immunohistochemistry screening at liver and kidney levels. TIL injection increased serum levels of hepatic and renal markers (ALP, ALT, AST, TC, TG, creatinine and urea) with a decrease in total proteins. In parallel, TIL induced hepatic and renal oxidative stress as there was an increase in malondialdehyde levels, with a decrease in catalase and reduced glutathione activities. Of interest, pre-administration of RHO inhibited TIL-induced increase in hepato-renal markers and decrease the oxidative stress and increased antioxidant activities of both liver and kidney. Quantitative RT-PCR showed that TIL increased HSP70 (heat shock protein), NFkB and TNF-α mRNA expression in liver. Moreover, TIL upregulated the expression of desmin, nestin, and vimentin expression in kidney. The upregulated genes were decreased significantly in the protective group received RHO. Serum inflammatory cytokines, together with genes of inflammatory markers in liver tissues (HSP70, NFkB and TNF-α) and in kidney tissues (desmin, nestin, and vimentin) were all affected. TIL induced hepatic vacuolation and congestion together with glomerular atrophy. The immunoreactivity of PCNA and HMGB1 were examined immunohistochemically. At cellular levels, PCNA was decreased while HMGB1 immunoreactivity was increased in TIL injected rats and was improved by pre administration of RHO. RHO administration protected the altered changes in liver and renal histology. Current findings support the possible use of RHO to shield the liver and kidney from the negative effects of tilmicosin.
Some properties of the novel topological graph index Atom-Bond Sum-Connectivity index
Authors: Jayanna Gowtham Kalkere , Swamy Narahari Narasimha , Sooryanarayana Badekara , Cangul Ismail Naci 
Published: March 2024

Abstract
The sum-connectivity variant of atom-bond connectivity index is recently introduced, and is defined as the sum of the terms of the form $\sqrt{(d_u+d_v-2)/(d_u+d_v)}$ over all adjacent vertices $u, v$ in a graph $G$, and named as the atom-bond sum connectivity index denoted by $ABS(G)$. The sum of the absolute values of all the eigenvalues of the adjacency matrix of a graph $G$ is known as the graph energy $E(G)$. In this paper, we observe some mathematical properties of $ABS(G)$ and establish bounds for this index in terms of other indices. We also compare $ABS(T)$ with $E(T)$ taking all trees $T$ of order $n=8,9,\cdots,14$ and observe a very strong correlation between the two invariants for all studied groups of trees.\\ \textbf{Keywords:} Atom-Bond Sum-Connectivity index, ABS, topological index, graph energy\\ \textbf{MSC 2010 Codes:} 05C07, 05C50
MSC 2010 Codes: 05C07, 05C50
Evaluation of the protective effect of cannabidiol against cadmium-induced toxicity in 2D and 3D model THLE-2 liver cell line
Authors: Şahin Serkan , Türksoy Vugar Ali , Azarkan Serap Yalçın 
Published: March 2024

Abstract
The likelihood of human exposure to toxic metals is increasing due to various factors. Among these toxic metals, cadmium (Cd) holds a significant place. New methods and substances are needed to protect individuals against the harmful effects caused by Cd. In the Transformed Human Liver Epithelial-2 (THLE-2) cell line, the cytotoxic effect of Cd (IC50) was determined. To assess the protective efficacy of cannabidiol (CBD), some groups of cell lines were treated with Cd alone, while others were subjected to a combination of Cd and 5 µM CBD. GSH, MDA, MPO, CAT, TNF-α, IL-1β, and IL-6 levels in cell lines were determined using ELISA kits. The inhibition of toxic metal entry into the cells by CBD was evaluated through ICP-MS analysis. The IC50 value for Cd was determined as 5 µM in 2D cell lines and 10 µM in 3D cell lines. It was observed that the application of CBD at a concentration of 5 µM, along with the determined IC50 dose of Cd, increased the cell proliferation rate. Additionally, the decrease in GSH and CAT levels, and the increase in MDA, MPO, TNF-α, IL-1β, and IL-6 levels observed in cell lines treated only with Cd were reversed with the application of CBD. The ICP-MS analysis revealed that CBD reduced the uptake of Cd into the cells. CBD reverses the oxidative stress and inflammation caused by Cd, increases cell proliferation, and reduces Cd entry into the cell. This provides strong evidence for the protective role of CBD against Cd exposures.
Flood induced buttertub spill reveals riverine plastic transport dynamics
Authors: Hauk Rahel , Ploeg Martine van der , Teuling Adriaan J. , de Winter Winnie , Emmerik Tim H.M. van 
Published: March 2024

Abstract

During the July 2021 European floods approximately 8 million empty dairy packaging (buttertubs) were flushed from a dairy processing facility in Belgium into the Vesdre river. Some were transported further downstream, into the Ourthe river and eventually the Meuse river. There are many unknowns when it comes to plastic transport in rivers, especially in response to floods. We therefore used this incident as an unique opportunity to study these buttertubs as a tracer for plastic transport dynamics in a riverine environment in response to an extreme flood event. Normally, it is unknown when and where individual plastic items found on riverbanks entered the environment. In this case, however, the ID stamps on the buttercups allowed for them to be traced back to the flooding of the factory. We studied the transport and deposition of these buttertubs in the Dutch Meuse over two years following the flood. We also collected buttertubs at different points in time to investigate their fragmentation and mass loss. Within three weeks of the flood, the buttertubs were transported up to 328 km from the spilling location. Overall, the majority (78%) of buttertubs we found within the first three weeks were deposited within less than 100 km of the point of emission. Over the following two years, the mean transport distance of the found buttertubs moved downstream from 100 km in July/August 2021, to 153 km in July 2023. The buttertubs average transport velocity decreased from 11.7 km/d within the first three weeks, to 0.2 km/d by July 2023. Based on the 89 buttertubs we collected and analyzed in detail over the two years, we did not find a significant mass loss. Of all 89 buttertubs found, 47 showed cracks and only 12 appeared to have pieces missing. This study shows that even during extreme flood events, the majority of spilled plastic litter is retained within a limited distance after being emitted into the river. The findings of this study can be utilized to improve plastic transport modelling, and overall better understand plastic transport in the freshwater environment.
mm wave based Gesture Recognition through FMCW Radar using Deep Learning Techniques
Authors: Abbas Waseem , Hussain Waqar 
Published: March 2024

Abstract
This research work employed machine learning models for hand gesture recognition using mmwave radar signals to classify different ergonomic and intuitive hand gestures. The proposed system uses IWR6843 AOP EVM frequency modulated continuous wave radar that helps in collecting the hand gestures data for the scalability purpose. The dataset consists of 2718 samples having six classes named as button press, circle, hand down, hand up, swipe left, and swipe right. These samples are collected from four individuals to train the deep neural network, recurrent neural networks, and transformer network. This study investigates the impact of various deep learning approaches on captured mmwave radar based data for gesture recognition. Moreover, a comparison is performed between the proposed methodology and state of the art study. The proposed methodology achieves a promising accuracy of 99.4% which shows that adopted model is robust, scalable and outperforms all other adopted models.
In silico genome wide identification of long non coding RNAs differentially expressed during Candida auris host pathogenesis
Authors: Mathur Kartavya , Singh Bharti , Puria Rekha , Nain Vikrant 
Published: March 2024

Abstract

Candida auris is an invasive fungal pathogen of high concern due to acquired drug tolerance against antifungals used in clinics. The prolonged persistence on biotic and abiotic surfaces can result in onset of hospital outbreaks causing serious health threat. An in depth understanding of pathology of C. auris is highly desirable for development of efficient therapeutics. Non coding RNAs play crucial role in fungal pathology. However, the information about ncRNAs is scanty to be utilized. Herein our aim is to identify long noncoding RNAs with potent role in pathobiology of C. auris. Thereby, we analyzed the transcriptomics data of C. auris infection in blood for identification of potential lncRNAs with regulatory role in determining invasion, survival or drug tolerance under infection conditions. Interestingly, we found 275 lncRNAs, out of which 253 matched with lncRNAs reported in Candidamine, corroborating for our accurate data analysis pipeline. Nevertheless, we obtained 23 novel lncRNAs not reported earlier. Three lncRNAs were found to be under expressed throughout the course of infection, in the transcriptomics data. 16 of potent lncRNAs were found to be coexpressed with coding genes, emphasizing for their functional role. Noteworthy, these ncRNAs are expressed from intergenic regions of the genes associated with transporters, metabolism, cell wall biogenesis. This study recommends for possible association between lncRNA expression and C. auris pathogenesis.
Synergizing Sensor Intelligence: a Novel Approach to Enhanced Human Activity Recognition
Authors: Karthik S. , Shanthi S. , Satish N. 
Published: March 2024

Abstract
Human activity recognition (HAR) methods are becoming increasingly crucial in observing daily human actions, namely aged care, investigations, intelligent homes, healthcare, and sports. Smart gadgets have various sensors, such as a gyroscope, motion, and accelerometer, which are extensively utilized inertial sensors that can detect various human physical circumstances. Many studies on human action recognition have been conducted recently. Smartphone sensor data generate high-dimensional relevant features that may be used to detect human actions. However, not all of the vectors are vital in the detection phase. The 'curse of dimensionality' occurs when all feature vectors are included. A hybridized feature selection technique that incorporates a wrapper and filter approach has been proposed in this study. The technique employs a sequential floating forward search (SFFS) with a Genetic Algorithm (GA) to extract the necessary characteristics for enhanced activity detection. The characteristics are then supplied into a fuzzy-based recurrent neural network (FRNN) classifier to generate nonlinear classifiers using deep learning features for training and testing. A benchmark dataset is utilized to investigate the proposed model. The suggested system utilizes limited hardware resources effectively and accurately identifies activities.