bluuebunny/crossref_metadata_2025_split huggingface dataset:
Creating Trauma and Disaster Informed Schools and Systems of Care for Underserved Youth through Mental Health Awareness Training in Puerto Rico
Authors: Orengo-Aguayo Rosaura , Stewart Regan W. , Sanfiorenzo Tania del Mar Rodríguez , González Karen G. Martínez 
Published: March 2024

Abstract
Climate change is disproportionately impacting the most vulnerable communities around the world and children are particularly at risk of experiencing adverse mental health impacts. Training the mental health and allied workforce in evidence-based models of care for affected youth is imperative. Mental health awareness training (MHAT) offers: 1) psychoeducation about various mental health topics, 2) tips and resources to help youth cope, and 3) referral information to connect youth with services. MHATs that are trauma and disaster-informed have been lacking, especially in Puerto Rico, a US territory severely impacted by extreme weather events. Our team at the [Removed for Masked Review] used a Community Based Participatory Research (CBPR) approach and partnered with two local universities in Puerto Rico [Removed for Masked Review] after the devastation of Hurricane Maria in 2017. Our collaborative needs assessment led to the following goals: 1) providing evidence-based, disaster and trauma-informed mental health awareness training; 2) identifying and connecting youth to mental health services; 3) implementing the first-ever, school-based telepsychiatry consultation program on the island; and 4) creating social media mental health awareness marketing campaigns to disseminate culturally and linguistically tailored resources. Over four years our team offered evidence-based, culturally and linguistically tailored MHATs including: Psychological First Aid (PFA), Trauma Informed Care (TIC), Suicide & Crisis Prevention and Response, Skills for Psychological Recovery (SPR), and Secondary Traumatic Stress and Self Careto 9,236 individuals utilizing in person and virtual training modalities. A total of 652 Puerto Rican youth were identified and referred to mental health services. Our trauma and disaster-informed mental health awareness training program was featured by the US Surgeon General as a potential model to help meet the mental health needs of disaster affected youth due to climate change.
Copper Oxide Nanoparticles (CuO NPs) prepared from Chitosan extract for antibacterial activity
Authors: Kadhim Faisal J. , Bedoui Mohamed Hedi 
Published: March 2024

Abstract
This research succeeded in the prepared of copper oxide nanoparticles (CuO NPs) via mixing (Cu2NO3) salt with chitosan extract using a hydrothermal method. The CuO NPs play a dominant role in the antibacterial process, eliminating microbes and producing an environment free of diseases. CuO NPs were examined via (XRD), (FE-SEM), UV-visible and PL spectrum. The XRD results showed a crystal size of (9.3 to 18) nm with face center cubic (FCC) structure for CuO NPs, and the diffraction peaks can be assigned with good crystal quality for CuO NPs. In addition, the FE-SEM results revealed a small grain size of CuO NPs — around 15 to 33 nm. UV-visible spectra show the energy band gap is 1.8 eV. The inhibition of zones, which were 40 mm for gram-positive bacteria (Staphylococcus aureus) and 40 mm for gram-negative bacteria (Klbesia), was used to determine the antibacterial qualities of CuO NPs.
Mechanical, Morphological and Thermal Properties of Thermoplastic Copolyester Elastomer Composites Filled with Calcite
Authors: Sucu Yunus Emre , Doganci Merve Dandan 
Published: March 2024

Abstract
In this study, the effects of different concentrations of stearic acid-coated calcite (CaCO3) on the mechanical, thermal, and morphological properties of thermoplastic polyester elastomer (COPE or TPE-E) were investigated. At the same time, COPEs, which consist of process wastes, that are qualified as PIR (postindustrial recycled), were physically recycled. 100% recycled polymer composites were obtained by blending the physically recycled COPE polymer with stearic acid-coated calcite at different concentrations. COPE composites (virgin and PIR) containing different concentrations of calcite (5 to 30%wt) were prepared by melt compounding. It has been determined that mechanical properties such as flexural strength and modulus increase with calcite concentration, while tensile strength decreases at higher concentrations owing to the stronger interfacial relations between polymer matrix and stearic acid-coated calcite. It was observed that the thermal properties of the composite increased with the calcite filler concentration. Morphological studies revealed a good dispersion of calcite fillers at lower concentrations in the polymer matrix.
Integration of ATAC-seq and RNA-seq Reveals the Dynamics of Chromatin Accessibility and Gene Expression in Zoysiagrass Response to Drought
Authors: Shen Liangying , Li Shuwen , Ai Ye , Zhang Jiahang , Qi Zewen , Xu Lixin , Han Liebao , Chao Yuehui , Wang Mengdi 
Published: March 2024

Abstract

Background
Zoysiagrass is renowned for its drought resistance and serves as an exceptional domestic turfgrass in China. However, the changes in chromatin accessibility during drought in zoysiagrass are not well understood.
Methods
We assessed the drought tolerance of six ecotypes zoysiagrass varieties based on their growth characteristics and physiological traits under drought conditions. Additionally, we utilized an integrated multi-omics strategy, encompassing whole-genome sequencing (WGS), RNA sequencing (RNA-seq), Assay for Transposase Accessible Chromatin using high-throughput sequencing (ATAC-seq), and RT-qPCR verification experiments, to gain a deeper understanding of the chromatin accessibility patterns linked to gene expression in response to drought stress in zoysiagrass.
Results
The correlation analysis between proline levels and drought tolerance in zoysiagrass revealed that the variety 'X4' exhibited notably high drought resistance compared to the other six zoysiagrass varieties. The KEGG pathway enrichment analysis revealed that zoysiagrass responded to environmental stress by regulating stress response and antioxidant defense pathways. Notably, the expression levels of genes Zja03G031540 and Zja11G000860 were notably increased in the 'X4' zoysiagrass genotype with improved drought tolerance compared to the 'X1' zoysiagrass genotype with reduced drought tolerance. This study suggested that 63 high-confidence genes related to drought stress and 6 motifs regulating drought responses were unearthed. The study discovered a positive correlation between ATAC-seq peak intensity and gene expression levels. The expression of high-confidence genes was linked to zoysiagrass resistance evaluation and phenotypic traits, implying that these genes are involved in responding to external drought stress.
Conclusions
This study combined ATAC-seq and RNA-seq technologies for the first time to identify drought-related genes expression in zoysiagrass, elucidating the grass adaptation to environmental stress and the regulatory mechanisms underlying stress responses, and laying the groundwork for zoysiagrass improvement and breeding.
A Novel Smartphone Application for Real-Time Localization and Tracking for Visually Impaired Individuals
Authors: Bougheloum Loubna , Salah Mounir Bousbia , Bettayeb Maamar 
Published: March 2024

Abstract

This paper aims to address the distinctive challenges faced by individuals with visual impairment concerning mobility and safety. It presents an advanced Android application named "Follow Me," developed using the MIT App Inventor platform. The primary objective of this application is to tackle these challenges by delivering real-time tracking and location sharing features for visually impaired individuals. Leveraging the capabilities of modern smartphones, this innovative app enables the continuous transmission of SMS messages containing the user's current location to a designated guardian. By following principles of design focused on the user's needs, the application's interface prioritizes accessibility and adaptability, guaranteeing a seamless experience for visually impaired users. Notably, it seamlessly integrates with Google Voice Assistant, allowing users to effortlessly activate or deactivate the tracking system using voice commands. The paper encompasses a comprehensive evaluation, including usability assessments, trials of SMS communication, location and tracking tests, and a comparative analysis with previous tracking systems and commercial applications. This ground-breaking application, "Follow Me," holds significant promise in enhancing the lives of individuals with visual impairments.
Vestibular schwannoma microneurosurgery in patients over 70: a single institution experience and proposal of a treatment algorithm
Authors: Michelini Silvia , Campione Alberto , Carpineta Ettore , Fraschetti Flavia , Scavo Carlo Giacobbo , Boccacci Fabio , Cacciotti Guglielmo , Stati Giovanni , Roperto Raffaelino , Alomari Amer A. , Mastronardi Luciano 
Published: March 2024

Abstract

Background. Elderly patients with vestibular schwannoma (VS) are commonly observed. Object. Retrospective analysis of 25 patients aging ≥ 70 operated on in our neurosurgical department for unilateral VS.
Methods. American Society of Anesthesiology (ASA) Grade I-II patients and Grade III with life-threatening tumors were enrolled. Karnofsky Performance Status Scale (KPS) was used for evalutation of the quality of life. The House-Brackmann (HB) scale for facial nerve (FN) outcome was used. Tumor size was categorized according to Koos’ classification. A retrosigmoid approach was used in all cases, except one in which a translabyrinthine approach was performed. Surgical removal graduation: total (GTR), near total (NT>95%), subtotal (ST>90%). Results. Mean age: 74,4 years (70-83); 28% ASA I, 56% ASA II, 16% ASA III. Mean tumor size: 2,7cm (1,5-4,2cm). GTR/NT resection: 68%, ST 32%. Mortality was zero. Long-term FN results: HBI 68%, HBII 8%, HBIII 12%; HB IV 12% (4 cases preoperatively). Transient complications occurred only in large VS. Re-growth of residue after ST was observed in 3 cases, treated with SRS in 2 cases and observed in 1.
Conclusions. An algorithm of treatment of vestibular schwannoma in the elderly is proposed. In particular, in patients in general good conditions, age does not appear to be a major contraindication for microsurgery of VS. Long-term FN results are satisfactory and the complication rates are acceptable.
General trends in irrigation practice during endodontic treatment among general dental practitioners in Malaysia.
Authors: bhatia shekhar , Chiew Kai Qi , Lim XIn Ni , Chhabra Naveen 
Published: March 2024

Abstract

Objective(s): This questionnaire study aimed to analyze the irrigation knowledge and current trends in irrigation practice during endodontic treatment among general dental practitioners (GDPs) in Malaysia.
Materials and Methods: A three-part self-administered questionnaire with multiple choice questions was distributed to dentists in Malaysia. Questions were segregated based on demographic data, irrigant selection and protocol, and other irrigation practices. Knowledge questions were marked based on a recommended answer by endodontists and the scores were analyzed among different groups with levels of experience using Kruskal-Wallis test.
Results: Out of 172 responses, knowledge scores of groups of practitioners with a different number of years of experience, the mean score of all respondents was 12.92 with a standard deviation of 2.774. For the scores of the groups of practitioners with different years of experience, Kruskal-Wallis test showed p=0.069.Sodium hypochlorite appeared as the most used irrigant. Most respondents routinely practiced smear layer removal using EDTA. Antibacterial capability and tissue dissolution were the main factors in irrigant selection. 65.6% of the GDPs claimed to change their irrigation protocol to suit different pulpal and periapical diagnoses. There was considerable variation regarding the type of irrigants used, irrigation protocol, concentration, volume, time spent for irrigation, irrigation needle usage, and additional adjunct methods used.
Conclusion(s): GDPs are currently following the recommended irrigation practices in terms of irrigant selection but there is scope for improvement in other aspects of irrigation practice. p>0.05 so years of experience is not a predictor for level of knowledge of irrigation protocol.
Long-term longitudinal changes in astigmatism and their effect on refraction development in Chinese clinical school-age children
Authors: Qin Zhuoer , zheng Qiaolan , Yu Xinping , Chen Jie , Chen Wuhe 
Published: March 2024

Abstract
Objectives
To investigate longitudinal changes in astigmatism in Chinese clinical school-age children and to explore the effect of astigmatism on refraction development.
Methods
The medical records of patients with long-term follow-up data from 2006 to 2018 were retrospectively reviewed. Patients who were 6–10 years old at initial visit and 16 years old at last assessment were selected for analysis. The enrolled patients had a cylinder refraction of 0.75 or greater. Astigmatism was analyzed in clinical notation and vector notation (J0, J45). The related factors of changes in astigmatism and spherical equivalent per year and the interaction between the two were analyzed.
Results
A total of 3101 patients (median age 9 years at initial visit) were followed up for an average of 7 years (IQR, 6–8 years). Astigmatism increased with age in low astigmats (< 1.50 D, 0.025 D/y) and decreased with age in high astigmats (≥ 3.00 D, -0.048 D/y). The oblique astigmatism (J45, 0.005D/y) increased and with-the-rule (WTR) astigmatism (J0, -0.008D/y) decreased. Higher myopia of the SE at the initial visit was associated with a greater increase in astigmatism magnitude(p < 0.001). A higher magnitude of initial astigmatism was associated with less progression in spherical equivalent(p < 0.001).
Conclusion
In Chinese clinical school-age children, the longitudinal development of astigmatism from 6–10 to 16 years of age varied with baseline astigmatism. The presence of myopia at baseline was a risk factor for astigmatism progression. However, high astigmatism seems to prevent the progression of myopia.
“Prevalence of dental fluorosis in permanent teeth among school going children in Bhavnagar district – A cross sectional study“
Authors: MISHRA MAYUR 
Published: March 2024

Abstract

Dental fluorosis is a major endemic oral disease characterized by hypomineralization of enamel caused due to consumption of water containing high concentration of fluoride during developmental stages of teeth. Fluorosis is one of the severe public health problems in India.Bhavnagar district is one among them, where people are affected with dental and skeletal fluorosis.Aim:To assess the prevalence of dental fluorosis in permanent teeth among 06-12 years old school children in endemic fluoride areas of Bhavnagar district.Methodology:A cross-sectional study was carried out in Bhavnagar district to assess the prevalence of dental fluorosis among 1500 school children in the age group of 6-12 years. Data collected included estimation of fluoride in the drinking water supply and measurement of dental fluorosis in school children.Results:Among total population of 1500 children, 746 were males and 754 were females. It shows the prevalence of Dental Fluorosis among total population of 1500 children, out of which 304 (40.75%) were males with fluorosis & 338 (44.82%) were females with fluorosis. Chi square test Pearson’s correlation test were applied to obtain inferential data.Conclusion: Our findings showed that the risk of dental fluorosis was significantly higher in the areas showing more fluoride content in drinking water.
Engineering Coupled Consortia-Based Biosensors using Incoherent Feedforward Loops
Authors: Daniel Ramez , Huang Rongying , Kravchik Valeriia , Geva-Zatorsky Naama 
Published: March 2024

Abstract
Synthetic multicellular systems offer the potential for performing complex biological tasks through coordinated cell-to-cell communication. However, the engineering of these systems faces challenges, including managing population ratios, cell growth rates, and asynchronous activity. To minimize the impact of these factors on the multicellular design, we developed coupled bacterial consortia designed for multi-marker detection by employing a stable shared quorum sensing signal, enabling the simultaneous initiation of their activities. The shared signal is driven by an incoherent feedforward loop (IFFL) gene circuit capable of generating a consistently low-level plateau-like signal that persists over 20 cell generations. This stability ensures a consistent synthesis rate of molecules and prevents unintended amplification during signal transduction, allowing for the efficient simultaneous initiation of activities among consortium members. We examined the consortia-based biosensor by monitoring Lactate and Heme in humanized fecal samples for 40 hours. Our findings provide insights into the IFFL system's dynamic response and long-term temporal stability, making them valuable for biotechnological applications. Particularly during the scaling of synthetic multicellular systems, where maintaining consistent cell density ratios among different strains within consortia is critical.
Effect of sample thickness on quantifying moisture content in oil-paper Insulation by terahertz characteristics and the corresponding correction method
Authors: Hu Jinlei , Yang Lijun , He Yuxin , Wang Ke , Jiang Tao , Wang Jianyi , Zhang Xiaoqin 
Published: March 2024

Abstract

Terahertz spectral characteristics are closely related to the moisture content in the transformer oil–paper insulation, which has great potential in nondestructive testing. However, the difference in the thicknesses of oil–paper insulation samples affects the spectral characteristics and the quantitative relationship between spectral characteristic values and moisture content, limiting the practical application of the quantitative relationship. In this study, the quantitative relationship between the moisture content and absorption coefficient integral eigenvalues of the three thicknesses of insulating paper is first experimentally investigated to reveal the influence of thickness on eigenvalues. Theoretical analyses are also conducted to analyze the factors affecting eigenvalues. This study also proposes that the maximum detectable absorption coefficient of the transmissive terahertz and the surface scattering are the main reasons for the differences in the eigenvalues. Finally, the methods of determining the integration interval through the maximum absorption coefficient and subtracting the surface scattering are proposed to eliminate the effect of thickness on eigenvalues. Thus, these terahertz eigenvalues become universally applicable in assessing the water content of insulating paper samples with different thicknesses.
Genetic Association of Serum Calcium, Phosphate, Vitamin D, Parathyroid Hormone, and FGF23 with the Risk of Aortic Stenosis
Authors: Zhao Qinghao , Nie Wenchang , Dong Jiaming , Zhang Bowen , Tang Gongzheng , Hong Shenda , Liu Jian 
Published: March 2024

Abstract
Aim: Disorders of mineral metabolism, including elevated levels of serum calcium, phosphate, 25-hydroxyvitamin D (25OH-VitD), parathyroid hormone (PTH), and fibroblast growth factor 23 (FGF23), have been reported in patients with calcific aortic valve stenosis (CAVS). However, evidence of the causal role of mineral metabolism in CAVS is still lacking. We aimed to investigate the causality between mineral metabolism and CAVS.
Methods: A systematic pipeline combining Mendelian randomization (MR), Steiger directionality test, colocalization analysis, protein-protein network, and enrichment analysis was applied to investigate the causal effect. Genome-wide association study (GWAS) and protein quantitative trait loci data for mineral metabolism markers were extracted from large-scale meta-analyses. Summary statistics for CAVS were obtained from two independent GWAS datasets as discovery and replication cohorts (n=374,277 and 653,867).
Results: In MR analysis, genetic mimicry of serum FGF23 elevation was associated with increased CAVS risk [ORdiscovery=3.081 (1.649-5.760), Pdiscovery=4.21×10-4; ORreplication=2.280 (1.461-3.558), Preplication=2.82×10-4] without evidence of reverse causation (Psteiger=7.21×10-98). Strong colocalisation association with CAVS was observed for FGF23 expression in the blood (PP.H4 = 0.96). Additionally, we identified some protein-protein interactions between FGF23 and known CAVS causative genes. Serum calcium, phosphate, 25OH-VitD, and PTH failed to show causal effects on CAVS at Bonferroni-corrected significance (all P>0.05/5=0.01).
Conclusions: Elevated serum FGF23 level is a causal risk factor for CAVS, and its mechanism of action in CAVS development may be independent of its function in regulating mineral metabolism. Hence, FGF23 may serve as a circulating marker and a promising preventive target for CAVS, warranting further investigation.
Nanozyme-Enhanced Injectable Hydrogel for the Treatment of Osteoarthritis
Authors: Wang Hui , Li Zuhao , Liu Jiaqi , Chang Haoran , Wang Jincheng , Song Shanliang , Zhao Yue , Zhao Xin 
Published: March 2024

Abstract
The tissue pathological changes of osteoarthritis (OA) encompass degenerative alterations in articular cartilage, peripheral bone proliferation, and synovial inflammation. The progression of OA is dramatically accelerated by the reactive oxygen species (ROS)-induced apoptosis of chondrocytes and the inflammatory response of synovial macrophages. The bone joint microenvironment refers to the biological environment inside the bone joints, which impacts the survival and function of cells within the bone joints. The interaction between the imbalance of the microenvironment and excessive generation of ROS promotes the development of arthritis. To address this issue, we have developed an injectable hydrogel with catalase-mimic nanozyme activity as a therapeutic agent for OA. The prepared nanozyme-reinforced hydrogels capable of not only eliminating ROS and inflammatory cytokines but also inducing the transformation of M1 macrophages into M2 phenotype in the harsh microenvironment. The dual mechanism enhances the repair of cartilage and facilitates the reduction of osteophyte formation in OA by improving the microenvironment within the joints. Our research has introduced an innovative treatment strategy for the clinical management of OA, demonstrating significant potential for its application in treating OA.