bluuebunny/crossref_metadata_2025_split huggingface dataset:
Repetitive locomotor training and physiotherapy improve walking and basic activities of daily living after stroke: a single-blind, randomized multicentre trial (DEutsche GAngtrainerStudie, DEGAS)
Authors: Pohl M , Werner C , Holzgraefe M , Kroczek G , Wingendorf I , Hoölig G , Koch R , Hesse S 
Published: January 2007

Objective: To evaluate the effect of repetitive locomotor training on an electromechanical gait trainer plus physiotherapy in subacute stroke patients. Design: Randomized controlled trial. Setting: Four German neurological rehabilitation centres. Subjects: One hundred and fifty-five non-ambulatory patients (first-time stroke <60 days). Intervention: Group A received 20 min locomotor training and 25 min physiotherapy; group B had 45 min physiotherapy every week day for four weeks. Main outcome measures: Primary variables were gait ability (Functional Ambulation Category, 0-5) and the Barthel Index (0-100), blindly assessed at study onset, end, and six months later for follow-up. Responders to the therapy had to become ambulatory (Functional Ambulation Category 4 or 5) or reach a Barthel Index of ≥ 75. Secondary variables were walking velocity, endurance, mobility and leg power. Results: The intention-to-treat analysis revealed that significantly greater number of patients in group A could walk independently: 41 of 77 versus 17 of 78 in group B (P B < 0.0001) at treatment end. Also, significantly more group A patients had reached a Barthel Index ≥ 75: 44 of 77 versus 21 of 78 (P B < 0.0001). At six-month follow-up, the superior gait ability in group A persisted (54 of 77 versus 28 of 78, P B < 0.0001), while the Barthel Index responder rate did not differ. For all secondary variables, group A patients had improved significantly more (P B < 0.0001) during the treatment period, but not during follow-up. Conclusions: Intensive locomotor training plus physiotherapy resulted in a significantly better gait ability and daily living competence in subacute stroke patients compared with physiotherapy alone.
Results of a HOVON/SAKK donor versus no-donor analysis of myeloablative HLA-identical sibling stem cell transplantation in first remission acute myeloid leukemia in young and middle-aged adults: benefits for whom?
Authors: Cornelissen Jan J. , van Putten Wim L. J. , Verdonck Leo F. , Theobald Matthias , Jacky Emanuel , Daenen Simon M. G. , van Marwijk Kooy Marinus , Wijermans Pierre , Schouten Harry , Huijgens Peter C. , van der Lelie Hans , Fey Martin , Ferrant Augustin , Maertens Johan , Gratwohl Alois , Lowenberg Bob 
Published: January 2007

Abstract
The Dutch-Belgian Hemato-Oncology Cooperative Group and the Swiss Group for Clinical Cancer Research (HOVON-SAKK) collaborative study group evaluated outcome of patients (pts) with acute myeloid leukemia (AML) in first remission (CR1) entered in 3 consecutive studies according to a donor versus no-donor comparison. Between 1987 and 2004, 2287 pts were entered in these studies of whom 1032 pts (45%) without FAB M3 or t(15;17) were in CR1 after 2 cycles of chemotherapy, received consolidation treatment, and were younger than 55 years of age and therefore eligible for allogeneic hematopoietic stem cell transplantation (allo-SCT). An HLA-identical sibling donor was available for 326 pts (32%), whereas 599 pts (58%) lacked such a donor, and information was not available in 107 pts. Compliance with allo-SCT was 82% (268 of 326). Cumulative incidences of relapse were, respectively, 32% versus 59% for pts with versus those without a donor (P < .001). Despite more treatment-related mortality (TRM) in the donor group (21% versus 4%, P < .001), disease-free survival (DFS) appeared significantly better in the donor group (48% ± 3% versus 37% ± 2% in the no-donor group, P < .001). Following risk-group analysis, DFS was significantly better for pts with a donor and an intermediate- (P = .01) or poor-risk profile (P = .003) and also better in pts younger than 40 years of age (P < .001). We evaluated our results and those of the previous MRC, BGMT, and EORTC studies in a meta-analysis, which revealed a significant benefit of 12% in overall survival (OS) by donor availability for all patients with AML in CR1 without a favorable cytogenetic profile.
Nilotinib exerts equipotent antiproliferative effects to imatinib and does not induce apoptosis in CD34+ CML cells
Authors: Jørgensen Heather G. , Allan Elaine K. , Jordanides Niove E. , Mountford Joanne C. , Holyoake Tessa L. 
Published: January 2007

Abstract
Chronic myeloid leukemia (CML) stem and progenitor cells overexpress BcrAbl and are insensitive to imatinib mesylate (IM). We therefore investigated whether these cells were efficiently targeted by nilotinib. In K562, the inhibitory concentration (IC50) of nilotinib was 30 nM versus 600 nM for IM, consistent with its reported 20-fold-higher potency. However, in primary CD34+ CML cells, nilotinib and IM were equipotent for inhibition of BcrAbl activity, producing equivalent but incomplete reduction in CrkL phosphorylation at 5 μM. CML CD34+ cells were still able to expand over 72 hours with 5 μM of either drug, although there was a concentration-dependent restriction of amplification. As for IM, the most primitive cells (CFSEmax) persisted and accumulated over 72 hours with nilotinib and remained caspase-3 negative. Furthermore, nilotinib with IM led to further accumulation of this population, suggesting at least additive antiproliferative effects. These results confirmed that, like IM, the predominant effect of nilotinib is antiproliferative rather than proapoptotic.
Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner
Authors: Chen Ping-Yen , Chang Wun-Shaing W , Chou Ruey-Hwang , Lai Yiu-Kay , Lin Sheng-Chieh , Chi Chia-Yi , Wu Cheng-Wen 
Published: January 2007

Abstract

Background
Despite of the fact that mammalian genomes are far more spacious than prokaryotic genomes, recent nucleotide sequencing data have revealed that many mammalian genes are arranged in a head-to-head orientation and separated by a small intergenic sequence. Extensive studies on some of these neighboring genes, in particular homologous gene pairs, have shown that these genes are often co-expressed in a symmetric manner and regulated by a shared promoter region. Here we report the identification of two non-homologous brain disease-related genes, with one coding for a serine protease inhibitor (SERPINI1) and the other for a programmed cell death-related gene (PDCD10), being tightly linked together by an asymmetric bidirectional promoter in an evolutionarily conserved fashion. This asymmetric bidirectional promoter, in cooperation with some cis-acting elements, is responsible for the co-regulation of the gene expression pattern as well as the tissue specificity of SERPINI1 and PDCD10.

Results
While SERPINI1 is predominantly expressed in normal brain and down-regulated in brain tumors, PDCD10 is ubiquitously expressed in all normal tissues but its gene transcription becomes aberrant in different types of cancers. By measuring the luciferase activity in various cell lysates, their 851-bp intergenic sequence was shown to be capable of driving the reporter gene expression in either direction. A 175-bp fragment from nt 1 to 175 in the vicinity of PDCD10 was further determined to function as a minimal bidirectional promoter. A critical regulatory fragment, from nt 176-473 outside the minimal promoter in the intergenic region, was identified to contain a strong repressive element for SERPINI1 and an enhancer for PDCD10. These cis-acting elements may exist to help coordinate the expression and regulation of the two flanking genes.

Conclusion
For all non-homologous genes that have been described to be closely adjacent in the mammalian genomes, the intergenic region of the head-to-head PDCD10-SERPINI1 gene pair provides an interesting and informative example of a complex regulatory system that governs the expression of both genes not only through an asymmetric bidirectional promoter, but also through fine-tuned regulations with some cis-acting elements.
Metastatic prostate cancer masquerading clinically and radiologically as a primary caecal carcinoma
Authors: Kabeer Muhammad A , Lloyd-Davies Edward , Maskell Giles , Hohle Rolf , Mathew Joseph 
Published: January 2007

AbstractBackgroundProstatic carcinoma is the second most common cause of cancer-related deaths in males in the West. Approximately 20% of patients present with metastatic disease. We describe the case of a patient with metastatic prostate cancer to the bowel presenting clinically and radiologically as a primary caecal cancer.Case presentationA 72 year-old man presented with abdominal discomfort and a clinically palpable caecal mass and a firm nodule on his thigh, the latter behaving clinically and radiologically as a lipoma. Computed tomographic (CT) scan showed a luminally protuberant caecal mass with regional nodal involvement. The patient was being treated (Zoladex®) for prostatic cancer diagnosed 6 years previously and was known to have bony metastases. On admission his PSA was 245.4 nmol/ml. The patient underwent a right hemicolectomy. Histology showed a poorly differentiated adenocarcinoma which was PSA positive, confirming metastatic prostatic adenocarcinoma to the caecum. The patient underwent adjuvant chemotherapy and is free from recurrence a year later.ConclusionMetastasis of prostatic carcinoma to the bowel is a very rare occurrence and presents a challenging diagnosis. The diagnosis is supported by immunohistochemistry for PSA. The treatment for metastatic prostate cancer is mainly palliative.
Pneumatized superior turbinate as a cause of headache
Authors: Homsioglou Elias , Balatsouras Dimitrios G , Alexopoulos Gregory , Kaberos Antonis , Katotomichelakis Michael , Danielides Vassilios 
Published: January 2007

Abstract

Background
A pneumatized superior turbinate is a rare cause of headache. Nasal endoscopy alone, does not provide us with adequate information for this inaccessible area of the superior nasal cavity. A coronal computed tomography (CT) must be obtained to confirm the diagnosis.

Case presentation
We present a 40-year-old female with migraine-type headache and nasal obstruction. Nasal endoscopy revealed a mild septal deviation, a right middle concha bullosa and a paradoxically curved middle turbinate on the left side. Coronal CT-scan showed also the presence of a superior concha bullosa on the left, which was in close contact with the nasal septum. The patient underwent septoplasty and bilateral endoscopic sinus surgery, including partial removal of both the pneumatized middle turbinates in conjunction with gentle lateralization and resection of the lower half of the left superior turbinate. Prompt relief from headache and nasal symptoms was obtained.

Conclusion
Pneumatized superior concha causing migrainous headache is a rare finding. Endoscopic surgery may provide permanent relief of symptoms.
Prospects of micromass culture technology in tissue engineering
Authors: Handschel Jörg GK , Depprich Rita A , Kübler Norbert R , Wiesmann Hans-Peter , Ommerborn Michelle , Meyer Ulrich 
Published: January 2007

Abstract
Tissue engineering of bone and cartilage tissue for subsequent implantation is of growing interest in cranio- and maxillofacial surgery. Commonly it is performed by using cells coaxed with scaffolds. Recently, there is a controversy concerning the use of artificial scaffolds compared to the use of a natural matrix. Therefore, new approaches called micromass technology have been invented to overcome these problems by avoiding the need for scaffolds. Technically, cells are dissociated and the dispersed cells are then reaggregated into cellular spheres. The micromass technology approach enables investigators to follow tissue formation from single cell sources to organised spheres in a controlled environment. Thus, the inherent fundamentals of tissue engineering are better revealed. Additionally, as the newly formed tissue is devoid of an artificial material, it resembles more closely the in vivo situation. The purpose of this review is to provide an insight into the fundamentals and the technique of micromass cell culture used to study bone tissue engineering.
IGFBP3mRNA expression in benign and malignant breast tumors
Authors: Ren Zefang , Shin Aesun , Cai Qiuyin , Shu Xiao-Ou , Gao Yu-Tang , Zheng Wei 
Published: January 2007

Abstract
Introduction
Most previous studies have focused on evaluating the association between circulating insulin-like growth factor binding protein 3 (IGFBP-3) levels and breast cancer risk. Emerging evidence over the past few years suggests that IGFBP-3 may act directly on mammary epithelial cells.

Methods
To understand the role of IGFBP-3 in breast tumorigenesis, we investigated IGFBP3 mRNA expression levels in benign and malignant breast tumors and their adjacent normal tissues using real-time quantitative PCR.

Results
Cancer tissues had significantly lower IGFBP3 expression than benign tumor tissues (p < 0.001). IGFBP3 expressions in both tumor and adjacent tissues were higher in patients who had proliferative benign tumors than in those who had non-proliferative benign tumors. Among patients with benign breast disease, IGFBP3 expression in the tumor was significantly higher than that in their adjacent normal tissue. There were no apparent associations of IGFBP3 expression in cancer tissues with either overall survival or disease-free survival in a cohort of 521 patients with breast cancer.

Conclusion
Our findings suggest that the expression level of IGFBP3 in breast tissues may be involved in breast tumorigenesis.
Update of the Anopheles gambiaePEST genome assembly
Authors: Sharakhova Maria V , Hammond Martin P , Lobo Neil F , Krzywinski Jaroslaw , Unger Maria F , Hillenmeyer Maureen E , Bruggner Robert V , Birney Ewan , Collins Frank H 
Published: January 2007

Abstract
Background
The genome of Anopheles gambiae, the major vector of malaria, was sequenced and assembled in 2002. This initial genome assembly and analysis made available to the scientific community was complicated by the presence of assembly issues, such as scaffolds with no chromosomal location, no sequence data for the Y chromosome, haplotype polymorphisms resulting in two different genome assemblies in limited regions and contaminating bacterial DNA.

Results
Polytene chromosome in situ hybridization with cDNA clones was used to place 15 unmapped scaffolds (sizes totaling 5.34 Mbp) in the pericentromeric regions of the chromosomes and oriented a further 9 scaffolds. Additional analysis by in situ hybridization of bacterial artificial chromosome (BAC) clones placed 1.32 Mbp (5 scaffolds) in the physical gaps between scaffolds on euchromatic parts of the chromosomes. The Y chromosome sequence information (0.18 Mbp) remains highly incomplete and fragmented among 55 short scaffolds. Analysis of BAC end sequences showed that 22 inter-scaffold gaps were spanned by BAC clones. Unmapped scaffolds were also aligned to the chromosome assemblies in silico, identifying regions totaling 8.18 Mbp (144 scaffolds) that are probably represented in the genome project by two alternative assemblies. An additional 3.53 Mbp of alternative assembly was identified within mapped scaffolds. Scaffolds comprising 1.97 Mbp (679 small scaffolds) were identified as probably derived from contaminating bacterial DNA. In total, about 33% of previously unmapped sequences were placed on the chromosomes.

Conclusion
This study has used new approaches to improve the physical map and assembly of the A. gambiae genome.
DiscoverySpace: an interactive data analysis application
Authors: Robertson Neil , Oveisi-Fordorei Mehrdad , Zuyderduyn Scott D , Varhol Richard J , Fjell Christopher , Marra Marco , Jones Steven , Siddiqui Asim 
Published: January 2007

AbstractDiscoverySpace is a graphical application for bioinformatics data analysis. Users can seamlessly traverse references between biological databases and draw together annotations in an intuitive tabular interface. Datasets can be compared using a suite of novel tools to aid in the identification of significant patterns. DiscoverySpace is of broad utility and its particular strength is in the analysis of serial analysis of gene expression (SAGE) data. The application is freely available online.
How is persistent insomnia maintained? A prospective study on 50–60 years old adults in the general population
Authors: Jansson‐Fröjmark Markus , Lundquist Daniel , Lundquist Nina , Linton Steven J. 
Published: January 2007

Objectives. The purpose of this study was to examine whether arousal, distress and sleep‐related beliefs are related to the maintenance of insomnia in old adults.Design. From a randomly selected sample from the general population (N = 3,600; 50–60 years old), 2,239 participants filled out a baseline and 1‐year follow‐up survey.Methods. Logistic regressions were used to investigate whether psychological mechanisms were related to sleep status (insomnia: N = 230; poor sleep: N = 210; normal sleep: N = 658; good sleep: N = 253) over one year. Cluster analysis was employed to assess whether it was possible to classify the participants based on their profiles of psychological functioning.Results. The results showed that arousal, sleep‐related beliefs about future consequences and anxiety were significantly related to the maintenance of insomnia (14–66% of the variance). Out of the individuals with persistent insomnia, 67% belonged to a cluster characterized by high scores on arousal, sleep‐related beliefs and anxiety, 24% to a cluster defined by medium scores on the three mechanisms and 9% to a cluster characterized by low scores on the three mechanisms.Conclusions. This investigation shows not only that arousal, sleep‐related beliefs and anxiety are associated with the maintenance of persistent insomnia, but also that these mechanisms often co‐occur in individuals with persistent insomnia.
Guidelines for potentiometric measurements in suspensions Part A. The suspension effect (IUPAC Technical Report)
Authors: Oman Srecko F. , Camões M. Filomena , Powell Kipton J. , Rajagopalan Raj , Spitzer Petra 
Published: January 2007

An explanation of the origin and interpretation of the suspension effect (SE) is presented in accordance with "pH Measurement: IUPAC Recommendations 2002" [Pure Appl. Chem.74, 2169 (2002)]. It is based on an analysis of detailed schemes of suspension potentiometric cells and confirmed with experimental results. Historically, the term "suspension effect" evolved during attempts to determine electrochemically the thermodynamically defined activity of H+ (aq) in suspensions. The experimental SE arises also in determining other pIon values, analogous to pH values.The SE relates to the observation that for the potential generated when a pair of electrodes (e.g., reference electrode, RE, and glass electrode) is placed in a suspension, the measured cell voltage is different from that measured when they are both placed in the separate equilibrium solution (eqs). The SE is defined here as the sum of: (1) the difference between the mixed potential of the indicator electrode (IE) in a suspension and the IE potential placed in the separated eqs; and (2) the anomalous liquid junction potential of the RE placed in the suspension. It is not the consequence of a boundary potential between the sediment and its eqs in the suspension potentiometric cells as is stated in the current definition of the SE.
What Is Africa to Me? Knowledge Possession, Knowledge Production, and the Health of Our Bodies Politic in Africa and the Africa Diaspora
Authors: Busia Abena P. A. 
Published: January 2007

At home Death claimsTwo streams from women's eyesAnd many day-long dirges;Gnashes, red eyes and sighs from men,The wailing of drums and musketsAnd a procession of the townsfolkImpededOnly if the coffin decidesTo take one last look at the home.But here I seeThree cars in procession.The first holds three—A driver chatting gaily with a mate,And behind them, flowers on a bier.The second holds five, and the third too.In 1976, in the middle of what was for us a second exile, my father's younger brother died. This death was shortly followed by the news of the death of his 112-year-old aunt, the only grandmother we had ever known. In accordance with custom and tradition, a few weeks later, and against the odds, the family in Wenchi, a town in the Brong-Ahafo region in the middle of Ghana, managed to send to Papa in Standlake, a small village in the countryside west of Oxford, the fragment of the burial cloth that was his aunt's and which he would have received on the day of the burial, had he been there.
Visions of Apes, Reflections on Change: Telling Tales of Great Apes in Equatorial Africa
Authors: Giles-Vernick Tamara , Rupp Stephanie 
Published: January 2007

Abstract:This article explores stories that some central Africans in the middle Sangha River basin and in northern Gabon have told about gorillas and chimpanzees. Such tales have provided opportunities for Africans to debate the consequences of their engagements with outside people, resources, and processes. But their meanings have proliferated in different social, cultural, and historical contexts. Central Africans have used such stories to make claims about access to and control over human productive and reproductive labor, forest resources and spaces, and other forms of wealth; racial and ethnic relations; and human existence and death. These stories provide critical insights into the reasons people hunt or protect great apes, and they illuminate the complex social and political tensions generated by conservation interventions. Great ape tales thus offer conservationists insights into the challenges and promise of managing an important game population, as well as the potential social consequences of their interventions.
Robbyn R. Wacker, Karen A. Roberto, and Linda E. Piper. Community Resources for Older Adults: Programs and Services in an Era of Change (2nd ed.). Thousand Oaks, CA: Pine Forge Press, 2002.
Authors: Racher Fran 
Published: January 2007

RÉSUMÉL'objet de Community Resources for Older Adults: Programs and Services in an Era of Change est d'offrir un aperçu des ressources communautaires dont les aînés américains peuvent se prévaloir à l'heure actuelle. L'ouvrage décrit le continuum des services et fournit d'excellents exemples de pratiques exemplaires en vigueur en Amérique. Chaque chapitre étudie les enjeux que présente le maintien des programmes et propose des orientations pour l'avenir. Les activités d'apprentissage génériques et les études de cas facilitent le passage de la théorie à la pratique. On regrette toutefois que l'ouvrage ne parle pas des contextes et programmes d'autres pays, cela aurait permis de faire des comparaisons internationales et aurait intéressé un public plus nombreux.