bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the anti‐aging effects of Zinc‐α2‐glycoprotein peptide in clinical and in vitro study
Authors: Lee Sang Gyu , Ham Seoyoon , Lee Joohee , Jang Yujin , Suk Jangmi , Lee Young In , Lee Ju Hee 
Published: February 2024

AbstractBackgroundSkin aging, characterized by the deterioration of skin density and elasticity, is a common concern among individuals seeking to maintain a youthful appearance. Zinc‐α2‐glycoprotein (ZAG) is secreted by various body fluids, and is associated with lipolysis and identified as an atopic dermatitis biomarker. This study evaluated the potential of ZAG peptides, which exert multiple benefits such as anti‐aging.Materials and MethodsWe conducted a 4‐week clinical trial on patients with noticeable periorbital wrinkles (n = 22) using a ZAG peptide‐containing product. The effects of the products on skin density, elasticity, and the depth of periorbital wrinkles were evaluated using Cutometer Dual MPA580, Ultrascan, and Antera 3D CS, respectively. The effect of ZAG peptides on UVB‐treated keratinocyte cells was evaluated in vitro to understand the mechanisms underlying its effects against impaired skin barrier function, collagen degradation, and senescence. In addition, the effects of ZAG peptides on cell viability and expression of aging and skin barrier‐related genes were assessed using cell counting kit assay and quantitative reverse transcription‐polymerase chain reaction, respectively.ResultsThe patients demonstrated improved skin density, elasticity, and reduced periorbital wrinkles. Further, more than 85% patients scored the product as satisfactory regarding anti‐aging effects. Furthermore, ZAG peptides reduced SA‐β‐gal staining, downregulated the senescence‐related genes, and upregulated the skin barrier function‐related genes in UVB‐irradiated keratinocyte cells.ConclusionsOur clinical and in vitro findings showed that ZAG peptides exert anti‐aging effects and improve skin barrier functions, suggesting their promising potential as therapeutic agents to combat skin aging and improve skin health.
A vanishing theorem for varieties with finitely many solvable group orbits
Authors: Wang Yiyu 
Published: February 2024

AbstractWe reprove and generalize a result proved by Yavin that the intersection cohomology groups of a toric variety with coefficient in a nontrivial rank one local system vanish. We prove a similar vanishing result for a certain class of varieties on which a connected linear solvable group acts, including all spherical varieties.
Antimicrobial susceptibilities of
Campylobacter fetus:
report from a reference laboratory
Authors: Zhang Ming May , Melton Tanner A. , Akhtar Haris , Shah Aditya , Schuetz Audrey N. , Clement Josh , Stevens Ryan W. 
Published: February 2024

ABSTRACT

Campylobacter fetus
is known to cause human disease, particularly in elderly and immunocompromised hosts. There are limited published data for antimicrobial susceptibility patterns with this organism, and no interpretive criteria are available. We reviewed antimicrobial susceptibilities of
C. fetus
isolates tested at a tertiary care center and reference laboratory over an 11-year period.
C. fetus
isolates from patients treated at Mayo Clinic and those sent as referrals for identification and susceptibility were included. Antimicrobial susceptibility testing was performed using agar dilution for ciprofloxacin, doxycycline, erythromycin, gentamicin, meropenem, and tetracycline. Geographic distribution, culture source, organism minimal inhibitory concentration (MIC) distributions, and MIC
50
and MIC
90
were examined. Excluding duplicates, 105 unique isolates were identified from 110 positive cultures. Blood cultures represented the most common source, followed by body fluids, skin and soft tissue, and central nervous system. Gentamicin and meropenem had favorable MIC
50
and MIC
90
of 1 µg/mL. Ciprofloxacin demonstrated an MIC
50
of 1 µg/mL; however, the MIC
90
was >2 µg/mL. Erythromycin demonstrated MIC
50
and MIC
90
of 2 µg/mL. Tetracycline and doxycycline were tested on a limited number of isolates and showed a wide range of MICs. Gentamicin and meropenem demonstrated favorable MICs in
C. fetus
isolates. These may represent therapeutic options for consideration in serious
C. fetus
infections, pending susceptibility results. Ciprofloxacin, which showed variable results, may be more appropriate for use only after susceptibility testing.
C. fetus
interpretive criteria are needed to aid clinicians in selection of both empiric and definitive therapies.

IMPORTANCE

Our findings contribute to the scant literature on
Campylobacter fetus
antimicrobial susceptibility test results. We used a reference test method of agar dilution and provide MICs for a large number of organisms and antimicrobial agents.
Accommodation Industry its Strategic Business Practices that Survive in Times of Crisis
Authors: Andias Amiel B. 
Published: February 2024

This study examined the accommodation industry: its strategic business practices that survive in times of crisis of Dipolog City Zamboanga del Norte. The qualitative case study methodology employed to obtain an in-depth investigation of the real-time experiences of the research participants. The study anchored on Fred R. David's (2021) five strategic business management practices: leadership, people, operations, marketing, and financial performance. The study revealed that accommodations industry practicing democratic forms of leadership, hiring of employees based on skills, providing motivations to their employees, operating their business by giving customer satisfaction, advertising their products and services, and ensuring that they attain financial stability. Despite the size of these enterprises, they still carried out sustainability practices that revolve around social, economic, and environmental practices that corporate social responsibility. The study also found out that accommodation industry challenges imposed by external and internal factors. In order to survive the challenges, Accommodation Industry employed business-level strategies to develop competitive advantage in the market. It therefore recommended that accommodation industry develop and strengthen their strategic practices taking into consideration their corporate social responsibility.
Features of the Unpublished Katigbawan Festival Dance of Catigbian, Bohol
Authors: Cagulada Elian Rose , Bulilawa Rafunzel Y. 
Published: February 2024

The Katigbawan Festival in Catigbian, Bohol, is a celebration deeply rooted in the town's cultural heritage. Among its vibrant components is a dance that encapsulates the spirit and identity of the community. Over the years, cultural practices have undergone transformations, and without careful documentation, the essence of these dances may be lost. In an age of globalization, sustaining local cultural practices is critical to retaining a distinct identity. This study aimed to identify and analyze the distinguishing characteristics of Katigbawan Festival Dance; the symbolism it conveys in the costumes and dance equipment/props used; the purpose of creating the dance; the values demonstrated in the Katigbawan Dance Steps; and the preservation initiatives implemented by the LGU.
This study utilized qualitative descriptive research method with interview guide questions serving as data-gathering methods. The analysis finds that the Katigbawan Festival's basic dance steps are classified as festival dance, with motions arranged within time signatures of 2/2, 3/4, and 4/4. The research also sheds light on the symbiotic link that exists between the dance steps (Katigbi, Tigbaw, and Kabaw) and the matching costumes and props. These elements contain basic qualities such as thankfulness, ingenuity, power, and hard work. The costume represents the Carabao, also capturing the sense of nature which is the cave and the growth of the plant Katigbi. Dance equipment and props are artfully intertwined throughout the performance to represent the richness of natural resources.
Sex‐ and Gender‐Based Reporting in Antihypertensive Medication Literature Informing Hypertension Guidelines
Authors: Gulamhusein Nabilah , Turino Miranda Keila , Dumanski Sandra M. , González Bedat María Carlota , Ulasi Ifeoma , Conjeevaram Arvind , Ahmed Sofia B. 
Published: February 2024

Background
Hypertension is the leading modifiable cardiovascular risk factor with recognized sex‐ and gender‐based differences. We assessed the incorporation of sex and gender reporting in the antihypertensive medication literature informing hypertension guidelines.

Methods and Results
Literature cited in the International Society of Hypertension (2020), European Society of Cardiology/European Society of Hypertension (2018), American College of Cardiology/American Heart Association (2017), Latin American Society of Hypertension (2017), Pan‐African Society of Cardiology (2020), and Hypertension Canada (2020) guidelines was systematically reviewed. Observational studies, randomized controlled trials, and systematic reviews involving antihypertensive medications were included. Studies with participants of a single sex, guidelines, and commentaries were excluded. Data on study participation‐to‐prevalence ratio by sex, analysis of baseline demographics and study outcomes by sex, and stratification of adverse events by sex were extracted. Of 1659 unique citations, 331 studies met inclusion criteria. Of those, 81% reported the sex of participants, and 22% reported a male‐to‐female participation‐to‐prevalence ratio of 0.8 to 1.2. Three percent of studies stratified baseline characteristics by sex, and 20% considered sex during analysis through statistical adjustment or stratification. Although 32% of studies reported adverse events, only 0.6% stratified adverse events by sex. Most (58%) studies reporting sex/gender used sex and gender terms interchangeably.

Conclusions
Incorporation of sex‐ and gender‐based considerations in study population, analysis, or reporting of results and adverse events is not common in the antihypertensive medication literature informing international hypertension guidelines. Greater attention to sex‐ and gender‐based factors in research is required to optimally inform management of hypertension.
Proteomic Associations of Adverse Outcomes in Human Heart Failure
Authors: Dib Marie‐Joe , Levin Michael G. , Zhao Lei , Diab Ahmed , Wang Zhaoqing , Ebert Christina , Salman Oday , Azzo Joe David , Gan Sushrima , Zamani Payman , Cohen Jordana B. , Gill Dipender , Burgess Stephen , Zagkos Loukas , van Empel Vanessa , Richards A. Mark , Doughty Rob , Rietzschel Ernst R. , Kammerhoff Karl , Kvikstad Erika , Maranville Joseph , Schafer Peter , Seiffert Dietmar A. , Ramirez‐Valle Francisco , Gordon David A. , Chang Ching‐Pin , Javaheri Ali , Mann Douglas L. , Cappola Thomas P. , Chirinos Julio A. 
Published: February 2024

Background
Identifying novel molecular drivers of disease progression in heart failure (HF) is a high‐priority goal that may provide new therapeutic targets to improve patient outcomes. The authors investigated the relationship between plasma proteins and adverse outcomes in HF and their putative causal role using Mendelian randomization.

Methods and Results

The authors measured 4776 plasma proteins among 1964 participants with HF with a reduced left ventricular ejection fraction enrolled in PHFS (Penn Heart Failure Study). Assessed were the observational relationship between plasma proteins and (1) all‐cause death or (2) death or HF‐related hospital admission (DHFA). The authors replicated nominally significant associations in the Washington University HF registry (N=1080). Proteins significantly associated with outcomes were the subject of 2‐sample Mendelian randomization and colocalization analyses. After correction for multiple testing, 243 and 126 proteins were found to be significantly associated with death and DHFA, respectively. These included small ubiquitin–like modifier 2 (standardized hazard ratio [sHR], 1.56;
P
<0.0001), growth differentiation factor‐15 (sHR, 1.68;
P
<0.0001) for death, A disintegrin and metalloproteinase with thrombospondin motifs–like protein (sHR, 1.40;
P
<0.0001), and pulmonary‐associated surfactant protein C (sHR, 1.24;
P
<0.0001) for DHFA. In pathway analyses, top canonical pathways associated with death and DHFA included fibrotic, inflammatory, and coagulation pathways. Genomic analyses provided evidence of nominally significant associations between levels of 6 genetically predicted proteins with DHFA and 11 genetically predicted proteins with death.

Conclusions
This study implicates multiple novel proteins in HF and provides preliminary evidence of associations between genetically predicted plasma levels of 17 candidate proteins and the risk for adverse outcomes in human HF.
Potential Protein Signatures for Recurrence Prediction of Ischemic Stroke
Authors: Zhang Chengyi , Liu Yang , Zhu Huimin , Huang Xinying , Guo Cang , Cheng Si , Yuan Meng , Jiang Yong , Meng Xia , Johnston S. Claiborne , Wang Yongjun , Jin Wei‐Na , Shi Fu‐Dong 
Published: February 2024

Background
Acute ischemic stroke is a major cause of mortality and disability worldwide, with approximately 7.4% to 7.7% recurrence within the first 3 months. This study aimed to identify potential biomarkers for predicting stroke recurrence.

Methods and Results
We conducted a nested case–control study using a hospital‐based cohort from the Third China National Stroke Registry selecting 214 age‐ and sex‐matched patients with ischemic stroke with hypertension and no history of diabetes or heart disease. Using data‐independent acquisition for discovery and multiple reaction monitoring for quantitative validation, we identified 26 differentially expressed proteins in large‐artery atherosclerosis (Causative Classification of Ischemic Stroke [CCS]1), 16 in small‐artery occlusion (CCS3), and 25 in undetermined causes (CCS5) among patients with recurrent stroke. In the CCS1 and CCS3 subgroups, differentially expressed proteins were associated with platelet aggregation, neuronal death/cerebroprotection, and immune response, whereas differentially expressed proteins in the CCS5 subgroup were linked to altered metabolic functions. Validated recurrence predictors included proteins associated with neutrophil activity and vascular inflammation (TAGLN2 [transgelin 2], ITGAM [integrin subunit α M]/TAGLN2 ratio, ITGAM/MYL9 [myosin light chain 9] ratio, TAGLN2/RSU1 [Ras suppressor protein 1] ratio) in the CCS3 subgroup and proteins associated with endothelial plasticity and blood–brain barrier integrity (ITGAM/MYL9 ratio and COL1A2 [collagen type I α 2 chain]/MYL9 ratio) in the CCS3 and CCS5 subgroups, respectively.

Conclusions
These findings provide a foundation for developing a blood‐based biomarker panel, using causative classifications, which may be used in routine clinical practice to predict stroke recurrence.
A rare case of an unexpected trigger of paroxysmal atrial fibrillation in the right atrial appendage diverticulum
Authors: Cheng Li , Hu Jianbo , Zhang Yuanping , Geng Zhaohua , Zhang Bo 
Published: February 2024

Abstract
Introduction
We described a rare case of an adolescent girl with paroxysmal atrial fibrillation originating from the right atrial appendage diverticulum and successfully converted to sinus rhythm after surgical intervention.

Methods
A 19-year-old girl was referred to the hospital for a catheter ablation of paroxysmal atrial fibrillation. conventional radiofrequency ablation using 3-D mapping were ineffective. Activation mapping showed the root of the free wall atrial appendage was first excited and catheter modeling (3D Carto map) showed a sac-like structure.

Results
We did selective angiography and further Computed tomography angiography (CTA) and Transesophageal echocardiography (TEE) which showed diverticulum originating from the right atrial appendage. Hence the patient was referred to cardiac surgery and had no recurrent atrial fibrillation at three months postoperative follow up.

Conclusions
Right atrial appendage diverticulum was an extremely rare malformation that can coexist with atrial tachyarrhythmia. Surgical ligation or excision of the abnormal structure with local ablation can achieve excellent results.
The response of CD27+CD38+ plasmablasts, CD24hiCD38hi transitional B cells, CXCR5−ICOS+PD-1+ Tph, Tph2 and Tfh2 subtypes to allergens in children with allergic asthma
Authors: Zhu Yunying , Jiang Qian , Lei Chenshuang , Yu Qinhua , Qiu Liannv 
Published: February 2024

Abstract
Background
Allergic asthma is a type I allergic reaction mediated by serum Immunoglobulin E (IgE). B cell-mediated humoral immune response to allergens in the pathophysiology of allergic asthma have not been thoroughly elucidated. Peripheral helper T cells (Tph) and follicular helper T cells (Tfh) promote B cell differentiation and antibody production in inflamed tissues.

Objective
To investigate the roles of B cell subsets, Tph cell subsets and Tfh cell subsets in allergic immune responses.

Methods
Circulating B cell subsets, Tph cell subsets and Tfh cell subsets in 33 children with allergic asthma and 17 healthy children were analyzed using multicolor flow cytometry. The level of serum total IgE was also assessed.

Results
Our study found that CD27+CD38+ plasmablasts and CD24hiCD38hi transitional B cells increased and were correlated with serum total IgE level, CD27− naive B cells and CD24hiCD27+ B cells decreased in children with allergic asthma. CXCR5− Tph, CXCR5−ICOS+ Tph, CXCR5−ICOS+PD-1+ Tph, CXCR5+ICOS+ Tfh and CXCR5+ICOS+PD-1+ Tfh increased in children with allergic asthma. Further analysis showed increased Tph2, Tph17, Tfh2 and Tfh17 subtypes while decreased Tph1 and Tfh1 subtypes in children with allergic asthma. Most interestingly, Tph2 or Tfh2 subtypes had a positive correlation with serum total IgE level.

Conclusion
Overall, these results provide insight into the allergens elicited B, Tph or Tfh cell response and identify heretofore unappreciated CD24hiCD38hi transitional B cells, CD24hiCD27+ B cells, CXCR5− Tph, CXCR5−ICOS+PD-1+ Tph, Tph2 subtypes and Tfh2 subtypes response to allergens.
Comparative evaluation of multiparametric lumbar MRI radiomic models for detecting osteoporosis
Authors: Zhen Tao , Fang Jing , Hu Dacheng , Shen Qijun , Ruan Mei 
Published: February 2024

Abstract
Background
Osteoporosis is a serious global public health issue. Currently, there are few studies that explore the use of multiparametric MRI radiomics for osteoporosis detection. The purpose of this study was to compare the performance of radiomics features from multiple MRI sequences (T1WI, T2WI and T1WI combined with T2WI) for detecting osteoporosis in patients.

Methods
A retrospective analysis was performed on 160 patients who had undergone dual-energy X-ray absorptiometry(DXA) and lumbar magnetic resonance imaging (MRI) at our hospital. Among them, 86 patients were diagnosed with abnormal bone mass (osteoporosis or low bone mass), and 74 patients were diagnosed with normal bone mass based on the DXA results. Sagittal T1-and T2-weighted images of all patients were imported into the uAI Research Portal (United Imaging Intelligence) for image delineation and radiomics analysis, where a series of radiomic features were obtained. A radiomic model that included T1WI, T2WI, and T1WI+T2WI was established using features selected by LASSO regression. We used ROC curve analysis to evaluate the predictive efficacy of each model for identifying bone abnormalities and conducted decision curve analysis (DCA) to evaluate the net benefit of each model. Finally, we validated the model in a sample of 35 patients from different health care institution.

Results
The T1WI + T2WI radiomics model showed better screening performance for patients with abnormal bone mass. In the training group, the sensitivity was 0.758, the specificity was 0.78, and the accuracy was 0.768 (AUC =0.839, 95% CI=0.757-0.901). In the validation group, the sensitivity was 0.792, the specificity was 0.875, and the accuracy was 0.833 (AUC =0.86, 95% CI=0.73-0.943).The DCA also showed that the combined model had better net benefits. In the external validation group, the sensitivity was 0.764, the specificity was 0.833, and the accuracy was 0.8 (AUC =0.824, 95% CI 0.678-0.969).

Conclusions
Radiomics-based multiparametric MRI can be used for the quantitative analysis of lumbar MRI and for accurately screening patients with abnormal bone mass.
Free-hand technique of C7 pedicle screw insertion using a simply defined entry point without fluoroscopic guidance for cervical spondylotic myelopathy patients with C3 to C6 instrumented by lateral mass screws: a retrospective cohort study
Authors: Jiang Jun , Song Chen-yu , Wu Zheng-zheng , Xie Zuo-zhi , Shi Bo , Xu Tao , Wang Han , Qiu Yong , Wang Bin , Zhu Ze-zhang , Yu Yang 
Published: February 2024

Abstract
Background
Nowadays, both lateral mass screw (LMS) and pedicle screw were effective instrumentation for posterior stabilization of cervical spine. This study aims to evaluate the feasibility of a new free-hand technique of C7 pedicle screw insertion without fluoroscopic guidance for cervical spondylotic myelopathy (CSM) patients with C3 to C6 instrumented by lateral mass screws.

Methods
A total of 53 CSM patients underwent lateral mass screws instrumentation at C3 to C6 levels and pedicle screw instrumentation at C7 level were included. The preoperative 3-dimenional computed tomography (CT) reconstruction images of cervical spine were used to determine 2 different C7 pedicle screw trajectories. Trajectory A passed through the axis of the C7 pedicle while trajectory B selected the midpoint of the base of C7 superior facet as the entry point. All these 53 patients had the C7 pedicle screw inserted through trajectory B by free-hand without fluoroscopic guidance and the postoperative CT images were obtained to evaluate the accuracy of C7 pedicle screw insertion.

Results
Trajectory B had smaller transverse angle, smaller screw length, and smaller screw width but both similar sagittal angle and similar pedicle height when compared with trajectory A. A total of 106 pedicle screws were inserted at C7 through trajectory B and only 8 screws were displaced with the accuracy of screw placement as high as 92.5%.

Conclusion
In CSM patients with C3 to C6 instrumented by LMS, using trajectory B for C7 pedicle screw insertion is easy to both identify the entry point and facilitate the rod insertion.
Incidental detection of Cladosporium in cytology
Authors: Santosh Tummidi , Chakrabarti Indranil , Palit Aparna , Srivastava Sonakshi 
Published: February 2024

Abstract
Background
Fungal infection incidental detection is a common encounter in cytopathology practices. Detection of the fungal organisms and awareness of the morphological features are challenges for the cytopathologist.

Case presentation
We report a case of incidental detection of a fungal organism in a 67-year-old male patient with complaints of bilateral elbow joint swellings. Cytology was done and showed a fungal organism (Cladosporium sps.).

Conclusion
Fine needle aspiration cytology (FNAC) along with Rapid on-site evaluation (ROSE) is a rapid, minimally invasive technique used for the diagnosis and detection of various fungi / parasites leading to early and definitive treatment.