bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbon emissions reduction strategies and poverty alleviation in India
Authors: OJHA VIJAY P. 
Published: November 2008

ABSTRACTThis paper, based on a computable general equilibrium model of the Indian economy, shows that a domestic carbon tax policy that recycles carbon tax revenues to households imposes heavy costs in terms of lower economic growth and higher poverty. However, the decline in economic growth and rise in poverty can be minimized if the emissions restriction target is modest, and carbon tax revenues are transferred exclusively to the poor. India's participation in an internationally tradable emission permits regime with grandfathered emissions allocation is preferable to any domestic carbon tax option, provided the world market price of emission permits remains low. Even better would be if India participated in a global system of tradable emission permits with equal per capita emission entitlements. India would then be able to use the revenues garnered from the sale of surplus permits to speed up its economic growth and poverty reduction and yet keep its per capita emissions below the 1990 per capita global emissions level.
High diversity of cultivable heterotrophic bacteria in association with cyanobacterial water blooms
Authors: Berg Katri A , Lyra Christina , Sivonen Kaarina , Paulin Lars , Suomalainen Sini , Tuomi Pirjo , Rapala Jarkko 
Published: November 2008

Abstract
Cyanobacterial mass occurrences (water blooms) cause ecological, economic and health problems worldwide. Still, little is known about heterotrophic bacteria associated with cyanobacteria and the interactions between those organisms. We isolated 460 bacterial strains from more than 40 lakes and rivers (151 samples), Baltic Sea (32 samples) and treated drinking water of seven treatment plants (29 samples). The water bodies and the raw water of the treatment plants were frequently dominated by high numbers of cyanobacteria. Various growth media were used to isolate the strains. Analysis of partial 16S rRNA gene fragments (701–905 bp for 358 strains and 413–497 bp for 102 strains) classified the isolated bacteria as Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes and Deinococcus-Thermus. Some of these isolates represented possible new bacterial orders, families, genera or species. We isolated various potentially pathogenic bacteria, such as Aeromonas, Vibrio, Acinetobacter and Pseudomonas, that may cause adverse health effects in humans and animals and should be taken into consideration when assessing the risks caused by cyanobacterial blooms. Several strains also inhibited or enhanced the growth of cyanobacteria. Most of such strains had an enhancing effect on the cyanobacterial growth. Other isolates were affiliated with genera such as Sphingomonas or Flavobacterium, which include strains that are capable of degrading cyanobacterial toxins or other recalcitrant and problematic organic compounds. The isolated strains provide a large group of bacteria that could be used in assessing and controlling the harmful effects of cyanobacteria.
Phylogenetic diversity of Acidobacteria in a former agricultural soil
Authors: Kielak Anna , Pijl Agata S , van Veen Johannes A , Kowalchuk George A 
Published: November 2008

Abstract
Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P&lt;0.001). Bacterial libraries recovered the greatest diversity, and sequence examination suggested that sequence mismatches with the Acidobacteria-specific primers limited the coverage of the metagenome library screening and specific library approaches. Together, these results expand knowledge of the distribution and diversity of Acidobacteria in soil environments and highlight important technical considerations in the molecular analysis of Acidobacteria diversity.
Interaction of Salmonella enterica with basil and other salad leaves
Authors: Berger Cedric N , Shaw Robert K , Brown Derek J , Mather Henry , Clare Simon , Dougan Gordon , Pallen Mark J , Frankel Gad 
Published: November 2008

Abstract
Contaminated salad leaves have emerged as important vehicles for the transmission of enteric pathogens to humans. A recent outbreak of Salmonella enterica serovar Senftenberg (S. Senftenberg) in the United Kingdom has been traced to the consumption of contaminated basil. Using the outbreak strain of S. Senftenberg, we found that it binds to basil, lettuce, rocket and spinach leaves showing a pattern of diffuse adhesion. Flagella were seen linking S. Senftenberg to the leaf epidermis, and the deletion of fliC (encoding phase-1 flagella) resulted in a significantly reduced level of adhesion. In contrast, although flagella linking S. enterica serovar Typhimurium to the basil leaf epidermis were widespread, deletion of fliC did not affect leaf attachment levels. These results implicate the role of flagella in Salmonella leaf attachment and suggest that different Salmonella serovars use strain-specific mechanisms to attach to salad leaves.
Human dimensions of Marine Protected Areas
Authors: Charles Anthony , Wilson Lisette 
Published: November 2008

Abstract
Charles, A., and Wilson, L. 2009. Human dimensions of Marine Protected Areas. – ICES Journal of Marine Science, 66: 6–15. Planning, implementing, and managing Marine Protected Areas (MPAs) requires that attention be paid not only to the biological and oceanographic issues that influence the performance of the MPA, but equally to the human dimensions: social, economic, and institutional considerations that can dramatically affect the outcome of MPA implementation. This paper explores ten human dimensions that are basic to the acceptance and ultimate success of MPAs: objectives and attitudes, “entry points” for introducing MPAs, attachment to place, meaningful participation, effective governance, the “people side” of knowledge, the role of rights, concerns about displacement, MPA costs and benefits, and the bigger picture around MPAs. These people-orientated factors and their impact on the success and effectiveness of MPAs are examined in relation to experiences with MPAs globally, and in relation to two Canadian examples specifically, one coastal (Eastport, Newfoundland) and the other offshore (the Gully, Nova Scotia).
Protection of capsaicin against hypoxia–reoxygenation-induced apoptosis of rat hippocampal neurons
Authors: Guo Shi-Yin , Yang Guo-Ping , Jiang De-Jian , Wang Feng , Song Tao , Tan Xing-He , Sun Zhen-Qiu 
Published: November 2008

The aim of this study was to investigate the effect of capsaicin on hypoxia–reoxygenation (H/R)-induced apoptosis in primary rat hippocampal neurons. Three hours of hypoxia (1% O2) and subsequent reoxygenation for 24 h significantly increased the apoptotic death of hippocampal neurons, as evidenced by increases in both TUNEL-positive cell number and caspase-3 activity. Pretreatment with capsaicin (3–30 µmol/L) or the caspase-3-specific inhibitor acetyl-DEVD-CHO (100 µmol/L) markedly attenuated H/R-induced apoptosis in hippocampal neurons. Capsaicin also markedly induced the phosphorylation of Akt. The phosphoinositide 3-kinase (PI3K) inhibitor LY294002 (10 µmol/L) prevented any capsaicin-induced survival effect in hippocampal neurons. Intracellular levels of reactive oxygen species (ROS), which were greatly increased after H/R, were significantly inhibited by capsaicin, pyrrolidine dithiocarbamate (PDTC) (50 µmol/L), and LY294002. Taken together, these data suggest that capsaicin protects against H/R-induced apoptosis of hippocampal neurons via the PI3K/Akt-mediated signaling pathway, which is related to the inhibition of oxidative stress and caspase-3 activation.
Genistein inhibits the development of atherosclerosis via inhibiting NF-κB and VCAM-1 expression in LDLR knockout mice
Authors: Wang Juejin , Zhang Rongjian , Xu Youhua , Zhou Hong , Wang Bin , Li Shengnan 
Published: November 2008

Diet can be an important factor that influences risks for cardiovascular disease. Genistein (4′,5,7-trihydroxyisoflavone), rich in soy, is one candidate that may benefit the cardiovascular system. Here, we explored the effect of genistein in atherosclerosis (AS) development in an in vivo mouse model. Low-density lipoprotein receptor (LDLR) knockout mice were allocated to control, model, and genistein groups. Our results showed that genistein significantly reduced the formation and development of atherosclerotic plaques ((4.68 ± 1.18) ×106versus (6.65 ± 1.51) ×106µm2, p < 0.05). In the genistein group, compared with the model group, total antioxidant capacity (TAC) level was 85.5 ± 15.6 versus 203.4 ± 32.6 mmol/L (p < 0.01); malondialdehyde (MDA) level was 3.79 ± 0.28 versus 3.06 ± 0.31 mmol/L (p < 0.01), and superoxide dismutase (SOD) activity was 86.1 ± 6.1 versus 139.1 ± 25.1 U/mL (p < 0.01). Therefore, genistein was able to enhance serum antioxidative ability in our mouse model. Genistein had no influence, however, on serum cholesterol and lipid profiles. Genistein also markedly downregulated the expression of nuclear factor (NF)-κB and vascular cell adhesion molecule (VCAM)-1 in aortas of mice (p < 0.05). These observations suggest that genistein may inhibit AS in LDLR−/−mice via enhancing serum antioxidation and downregulating NF-κB and VCAM-1 expression in the aorta.
Rapid cardiovascular effects of dexamethasone in rabbits with meconium-induced acute lung injury
Authors: Mokra Daniela , Tonhajzerova Ingrid , Mokry Juraj , Drgova Anna , Petraskova Maria , Calkovska Andrea , Javorka Kamil 
Published: November 2008

Glucocorticoids may improve lung function in newborns with meconium aspiration syndrome (MAS), but information on the acute side effects of glucocorticoids in infants is limited. In this study using a rabbit model of MAS, we addressed the hypothesis that systemic administration of dexamethasone causes acute cardiovascular changes. Adult rabbits were treated with 2 intravenous doses of dexamethasone (0.5 mg/kg each) or saline at 0.5 h and 2.5 h after intratracheal instillation of human meconium or saline. Animals were oxygen-ventilated for 5 h after the first dose of treatment. Blood pressure, heart rate, and short-term heart rate variability (HRV) were analyzed during treatment, for 5 min immediately after each dose, and for the 5 h of the experiment. In the meconium-instilled animals, dexamethasone increased blood pressure, decreased heart rate, increased HRV parameters, and caused cardiac arrhythmia during and immediately after administration. In the saline-instilled animals, the effect of dexamethasone was inconsistent. In these animals, the acute effects of dexamethasone on blood pressure and cardiac rhythm were reversed after 30 min, whereas heart rate continued to decrease and HRV parameters continued to increase for 5 h after the first dose of dexamethasone. These effects were more pronounced in meconium-instilled animals. If systemic glucocorticoids are used in the treatment of MAS, cardiovascular side effects of glucocorticoids should be considered.
Comparative effects ofN-acetyl-l-cysteine and ramipril on arterial hypertension, insulin resistance, and oxidative stress in chronically glucose-fed rats
Authors: El Midaoui Adil , Ismael Mahmoud Ali , Lu Huogen , Fantus I. George , de Champlain Jacques , Couture Réjean 
Published: November 2008

Beneficial effects of an antioxidant (N-acetyl-l-cysteine, NAC) and an angiotensin I-converting enzyme (ACE) inhibitor (ramipril) were assessed in a rat model of insulin resistance induced by 10% glucose feeding for 20 weeks. Treatments with NAC (2 g/kg per day) and ramipril (1 mg/kg per day) were initiated at 16 weeks in the drinking fluid. Systolic blood pressure, plasma levels of insulin and glucose, and insulin resistance were significantly higher in rats treated with glucose for 20 weeks. This was associated with a higher production of superoxide anion and NADPH oxidase activity in aorta and liver and with a marked reduction in protein expression of skeletal muscle insulin receptor substrate-1 (IRS-1) in the gastrocnemius muscle. NAC prevented all these alterations. Although ramipril also reversed high blood pressure, it had a lesser effect on insulin resistance (including IRS-1) and blocked superoxide anion production only in aorta. Ramipril, in contrast to NAC, did not reduce NADPH oxidase activity in aorta and liver or plasma levels of 4-hydroxynonenal and malondialdehyde. Results suggest that the inhibition of the oxidative stress in hypertensive and insulin-resistant states contributes to the therapeutic effects of NAC and ramipril. Whereas NAC exerts effective antioxidant activity in multiple tissues, ramipril appears to preferentially target the vasculature.
MHD MODELS AND SYNTHETIC SYNCHROTRON MAPS FOR THE JET OF M87
Authors: GRACIA J. , BOGOVALOV S. V. , TSINGANOS K. 
Published: November 2008

We present a self-consistent MHD model for the jet of M87. The model consist of two distinct zones: an inner relativistic outflow, which we identify with the observed jet, and an outer cold disk wind. While the former does not self-collimate efficiently due to its high effective inertia, the latter fulfills all the conditions for efficient collimation by the magneto-centrifugal mechanism. Given the right balance between the effective inertia of the inner flow and the collimation efficiency of the outer disk wind, the relativistic flow is magnetically confined into a well collimated beam for a wide range of parameters and matches the measurements of the opening angle of M87 over several orders of magnitude in spatial extent. In the second part of this work, we present synthetic synchrotron emission maps for our MHD models. In principle, the two-zone model can reproduce the morphological structure seen in radio observations, as central-peaked profiles across the jet close the the source, limb-bright further down the jet, and a bright knot close to the position of HST-1. However it is difficult to reconcile all features with a single set of parameters.
MAGNETIC RECONNECTION AT THE TERMINATION SHOCK OF A STRIPED PULSAR WIND
Authors: PÉTRI JÉRÔME , LYUBARSKY YURI 
Published: November 2008

Most of the rotational luminosity of a pulsar is carried away by a relativistic magnetized wind in which the matter energy flux is negligible compared to the Poynting flux. However, observations indicate that most of the Poynting flux is eventually converted into ultrarelativistic particles. The mechanism responsible for this transformation remains poorly understood. Near the equatorial plane of an obliquely rotating pulsar magnetosphere, the magnetic field reverses polarity with the pulsar period, forming a wind with oppositely directed field lines, called a striped wind. We study the conditions required for magnetic energy release at the termination shock of the striped pulsar wind. Magnetic reconnection is considered via analytical methods and 1D relativistic PIC simulations. An analytical condition on the upstream parameters for partial and full magnetic reconnection is derived from the conservation laws of energy, momentum and particle number density across the relativistic shock. Furthermore, by using a 1D relativistic PIC code, we study in detail the reconnection process at the termination shock for different upstream Lorentz factors and magnetizations, and find good agreement with our analytical criterion. Thus, alternating magnetic fields annihilate easily at relativistic highly magnetized shocks. We apply our criterion for dissipation to deduce bounds on the pair multiplicity, κ, in the pulsar wind.
Lysosomal Cathepsin B Participates in the Podosome-Mediated Extracellular Matrix Degradation and Invasion via Secreted Lysosomes in v-Src Fibroblasts
Authors: Tu Chun , Ortega-Cava Cesar F. , Chen Gengsheng , Fernandes Norvin D. , Cavallo-Medved Dora , Sloane Bonnie F. , Band Vimla , Band Hamid 
Published: November 2008

Abstract
Podosomes mediate cell migration and invasion by coordinating the reorganization of actin cytoskeleton and focal matrix degradation. MMP and serine proteases have been found to function at podosomes. The lysosomal cysteine cathepsins, a third major class of matrix-degrading enzymes involved in tumor invasion and tissue remodeling, have yet to be linked to podosomes with the exception of cathepsin K in osteoclasts. Using inhibitors and shRNA-mediated depletion, we show that cathepsin B participates in podosomes-mediated focal matrix degradation and invasion in v-Src–transformed fibroblasts. We observed that lysosomal marker LAMP-1 localized at the center of podosome rosettes protruding into extracellular matrix using confocal microscopy. Time-lapse live-cell imaging revealed that lysosomal vesicles moved to and fused with podosomes. Disruption of lysosomal pH gradient with Bafilomycin A1, chloroquine, or ammonium chloride greatly enhanced the formation of podosomes and increased the matrix degradation. Live-cell imaging showed that actin structures, induced shortly after Bafilomycin A1 treatment, were closely associated with lysosomes. Overall, our results suggest that cathepsin B, delivered by lysosomal vesicles, is involved in the matrix degradtion of podosomes. [Cancer Res 2008;68(22):9147–56]
NOTCH Is Part of the Transcriptional Network Regulating Cell Growth and Survival in Mouse Plasmacytomas
Authors: Shin Dong-Mi , Shaffer Daniel J. , Wang Hongsheng , Roopenian Derry C. , Morse Herbert C. 
Published: November 2008

Abstract
Aside from Myc-activating translocations characteristic of plasmacytomas (PCT), little is known about genetic factors and signaling pathways responsible for the development of spontaneous B-cell lineage lymphomas of mice. Here, we characterized the transcriptional profiles of PCT, centroblastic diffuse large B-cell lymphomas (CBL), and high-grade splenic marginal zone B-cell lymphoma (MZL++) using high-throughput quantitative reverse transcription-PCR. Expression profiles of CBL and MZL++ were strikingly similar and quite unlike that of PCT. Among the genes expressed at significantly higher levels by PCT were a number involved in NOTCH signaling, a finding supported by gene set enrichment analyses of microarray data. To investigate the importance of this pathway, NOTCH signaling was blocked in PCT cell lines by treatment with a γ-secretase inhibitor (GSI) or transduction of a dominant-negative mutant of MAML1. These treatments resulted in reduced expression of NOTCH transcriptional targets in association with impaired proliferation and increased apoptosis. GSI treatment of transformed plasma cells in a primary PCT also induced apoptosis. These results integrate NOTCH activation with oncogenic signaling pathways downstream of translocated Myc in the pathogenesis of mouse PCT, two signaling pathways also implicated in development of human multiple myeloma and T-cell lymphoblastic lymphoma. [Cancer Res 2008;68(22):9202–11]
AKT1 Inhibits Homologous Recombination by Inducing Cytoplasmic Retention of BRCA1 and RAD51
Authors: Plo Isabelle , Laulier Corentin , Gauthier Laurent , Lebrun Fabienne , Calvo Fabien , Lopez Bernard S. 
Published: November 2008

Abstract
AKT1 is frequently up-regulated in sporadic breast cancer, whereas BRCA1 is frequently mutated in familial breast cancer. Because BRCA1 is involved in homologous recombination (HR), we addressed whether AKT1 also has an effect on this process. We showed that AKT1 repressed HR through cytoplasmic retention of BRCA1 and RAD51 proteins, resulting in a BRCA1-deficient–like phenotype. This process does not require direct BRCA1 phosphorylation by AKT1. The cytoplasmic retention of BRCA1 and RAD51 correlated with activated AKT1 in tumor cell lines and in biopsies from sporadic breast cancers. Under nonpathologic conditions, fibroblast growth factor, which activates AKT1 and stimulates proliferation in fibroblasts, impaired excessive HR without fully inhibiting it, promoting genome stability. Our study reveals that the regulation of BRCA1 and RAD51 is altered in a high frequency of sporadic breast cancers and highlights the role of extracellular AKT signaling-dependent regulation of HR and genome stability. [Cancer Res 2008;68(22):9404–12]
Type 2 Bias of T Cells Expanded from the Blood of Melanoma Patients Switched to Type 1 byIL-12p70mRNA–Transfected Dendritic Cells
Authors: Minkis Kira , Kavanagh Daniel G. , Alter Galit , Bogunovic Dusan , O'Neill David , Adams Sylvia , Pavlick Anna , Walker Bruce D. , Brockman Mark A. , Gandhi Rajesh T. , Bhardwaj Nina 
Published: November 2008

AbstractMelanoma patients may exhibit a TH2-skewed cytokine profile within blood and tumor-infiltrating lymphocytes. Therapies that induce beneficial TH1-type tumor-specific immune responses, therefore, are highly desirable. Dendritic cells (DC) are widely used as immune adjuvants for cancer. Before their administration, DC are generally induced to mature with a cocktail of recombinant cytokines [interleukin (IL)-1β, tumor necrosis factor α, and IL-6] and prostaglandin E2 (PGE2), which is added to preserve the ability of DC to migrate to draining lymph nodes. However, PGE2 suppresses the production of IL-12p70, a cytokine essential for differentiation of TH1 responses. In this study, human DC were transfected with IL-12p70 mRNA and tested for their ability to alter the TH2 type bias manifested by blood T cells of patients with melanoma. Transfected DC secreted high levels of bioactive IL-12p70, as indicated by their capacity to enhance natural killer cell activity, skew TH1 responses in allogeneic mixed lymphocyte reactions through reduction of IL-4 and IL-5, and prime CD8+ T cells to the melanoma-associated antigen Melan A/MART-1. Furthermore, T-cell lines primed in vitro from the blood of melanoma patients showed strong type 2 skewing that was dramatically reversed by IL-12p70 transfection of autologous DC. Thus, IL-12p70 transfection of clinical DC preparations may enhance type 1 antitumor responses and may thereby contribute to effective immune-based therapy. [Cancer Res 2008;68(22):9441–50]