bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methane Emission from Lakes in the North of Western Siberia
Authors: Kazantsev V. S. , Krivenok L. A. , Dvornikov Yu. A. , Lomov V. A. , Sabrekov A. F. 
Published: April 2024

The paper presents the results of field measurements of methane fluxes into the atmosphere from thermokarst lakes located on the Russian Federation territory on the three key sites: foothills of the Polar Urals, coast of the Kara Sea and the northwestern part of the Yamal Peninsula. A total of 13 lakes were studied and about 500 methane fluxes were measured by the floating chamber method. The results showed most of the fluxes does not exceed 8 mg CH4 m–2 h–1. For more significant values, a statistically significant correlation with the wind speed was revealed, which largely determines the intensity of gas exchange on the “water-atmosphere” boundary. The exceptions are measurements in zone of lake methane seeps. For most lakes, the greatest scatter of measured fluxes was observed in the shallow part. The diurnal dynamics of methane fluxes was approximated by a sinusoidal function. For the lakes presented in the work, the range of emission assessment is 0.23–775.38 g CH4 h–1. Obtained results are important material for estimating regional methane emission from the surface of thermokarst lakes in the tundra zone.
Analysis of the Features of Water Circulation in the Northern Part of the Black Sea for Different Seasons on the Basis of the Results of Numerical Modeling with the Assimilation of the Data of Hydrological Surveys in 2016
Authors: Demyshev S. G. , Evstigneeva N. A. 
Published: April 2024

The analysis of the dynamic and energy characteristics of water circulation in the northern part of the Black Sea was performed on the basis of the assimilation in the numerical model of the data of three hydrological surveys in 2016, carried out on expeditions of 87, 89 and 91 cruises of the R/V Professor Vodyanitsky (summer, autumn and autumn-winter seasons). Numerical experiments were implemented on a horizontal grid (~1.6 km × ~1.6 km) with 27 vertical horizons and an atmospheric effect close to the real one was used. A procedure of assimilation of the observational data was based on the Kalman filter, taking into account the heterogeneity and nonisotropy of the errors of the estimates of the temperature and salinity fields. The integral energy terms in the kinetic and potential energy budget equations for three seasons were estimated. In the summer season, there was a slight weakening of the RC and the main mechanism for the formation of anticyclonic eddies near Sevastopol and near the southeastern shores of Crimea was baroclinic instability of the current (as evidenced by the increase in the slope of isopycnical surfaces and negative values of the work of the buoyancy force). An anticyclonic eddy near Yalta with a radius of about 25 km was generated due to the development of shear instability of the current. In the autumn season, the RC jet was pressed to the shore and there was a decrease in the number of eddies in comparison to the summer season. The formation of anticyclonic eddies with a radius of about 35–40 km in the western part of the region was caused by barotropic instability of the current, the formation of eddies along the Crimean coast – by baroclinic instability. In the autumn-winter season, the RC had a pronounced jet character and there was an increase in the processes of baroclinic instability with the generation of eddies of different scales between the coast and the RC, as well as in the area, located between 31.5 and 33° E, with the weakening of the wind effect. During all seasons, small-scale anticyclonic and cyclonic eddies could be generated along the western and eastern coast of Crimea in the upper layer when current flowed around the coastline and inhomogeneities of the bottom topography under the action of weak winds.
Stability of the Vertical Distribution of Dust Aerosol in Light and Moderate Winds
Authors: Malinovskaya Е. A. , Chkhetiani O. G. , Golitsyn G. S. , Lebedev V. A. 
Published: April 2024

The profiles of dust aerosol mass concentration obtained with multilevel (0.2, 0.4, 0.8, 1.6, and 3.2 m) daytime measurements in arid conditions in 2020–2022 show a power dependence on height. We distinguish three main types of changes in concentration with height: a) in low wind (degrees are close to –0.5); b) spike changes in concentration when wind increases (degrees reach and exceed –1); c) inversions (concentration increases with height at two or three lower levels of measurements): weak – about –20 mkg/cm3, significant – more than 50 mkg/cm3. The power dependence of –0.5 is explained by the collective effect of the rise of the ensemble of closely located bubbles of air warmed around the dust particles. In weak and moderate winds, this mode is more common. Burst changes in concentration are associated with the emergence of turbulent structures.
Post-Sedimentation Transformations in Silurian Carbonate Rocks of the Central Part of the Chernyshev Ridge (Timan-Pechora Province)
Authors: Danshikova I. I. , Maydl T. V. 
Published: April 2024

The article presents the results of studies of post-sedimentary processes in Silurian carbonate rocks of the central part of the Chernyshev Ridge. A complex combination of secondary transformations in carbonate re-servoir is shown. The sedimentary strata show manifestations of regional and superimposed lithogenesis. In addition to stage-catagenetic changes, the sediments were also exposed to regressive infiltration catagenesis (epigenesis), accompanied by leaching and dolomitization of limestone. All this significantly affected the filtration-capacitative properties. The obtained data can contribute to the identification of new objects for exploration drilling and optimization of exploration works in the complex territories.
Physiological Characteristics of <i>Saccharomyces cerevisiae</i> Strain Overexpressing Polyphosphatase Ppx1
Authors: Trilisenko L. V. , Valiakhmetov A. Ya. , Kulakovskaya T. V. 
Published: April 2024

Abstract—The Ррх1 exopolyphosphatase of yeast is a constitutive protein localized predominantly in the cytoplasm. The purified enzyme hydrolyzes inorganic polyphosphates with high activity; however, in the knockout ∆ppx1 mutant of Saccharomyces cerevisiae the increase in the polyphosphate level was small, and no changes in physiological properties of this mutant were observed. To elucidate the functions of Ppx1, we studied the physiological characteristics of the S. cerevisiae strain overexpressing this enzyme. When cultivated in the YPD medium, the strain overexpressing Ppx1 showed no growth features different from those of the parental strain. The following physiological features of the strain overexpressing Ppx1 were observed at the stationary stage of growth: the level of ATP increased by nine times, the activity of vacuolar ATPase significantly decreased, and the sensitivity to peroxide increased compared to the parental strain. The level of reactive oxygen species doubled, while the degree of lipid oxidation remained the same as in parental strain. Since overexpression of Ppx1 under the culture conditions used did not affect the polyphosphate level, these polymers were not the regulators of the changes described above. Response to oxidative stress and vacuolar ATPase activity in yeasts is known to be regulated by cAMP, while Ppx1 is capable of hydrolyzing this signaling compound. We suggest that one of the functions of Ppx1 in yeasts is participation in the regulation of cAMP level.
Biodiversity and Metabolic Properties of Bacterial Communities from the Digestive System of the Bivalve <i>Crenomytilus grayanus</i>
Authors: Dunkai T. I. , Bogatyrenko E. A. , Kim A. V. 
Published: April 2024

Abstract—Biodiversity of the bacterial communities in the digestive system of Crenomytilus grayanus inhabiting the coastal Sea of Japan waters with chronic anhropogenic pollution was investigated using metabarcoding. Apart from marine bacteria, the taxa typical under contamination with oil (Rhodobacteraceae, Corynebacteriaceae), heavy metals (Asinibacterium), and unprocessed municipal waste (Cloacibacterium, Globicatella) were revealed in the microbiota. A collection of 411 cultured heterotrophic bacterial strains isolated in the course of this study was characterized taxonomically. The intestinal microbiome of the studied mollusks was shown to have a unique composition, depending on their habitat. Ability of bacterial strains isolated from the C. grayanus digestive system to degrade various nutrient substrates (sugars, amino acids, and polysaccharides) and xenobiotics (oil hydrocarbons, bisphenol A, and atrazine) was studied. Most isolates degraded a broad range oforganic substrates; 13% (54 strains) oxidized oil hydrocarbons; 1% (4 strains) oxidized bisphenol A; and 0.5% (2 strains) degraded atrazine. The possible role of the microbiome C. grayanus microbiome in symbiotic digestion and in detoxication of the mollusk is discussed.
<i>Paludisphaera mucosa</i> sp. nov., a Novel Planctomycete of the Family <i>Isosphaeraceae</i> from a Boreal Fen
Authors: Ivanova A. A. , Naumoff D. G. , Kulichevskaya I. S. , Meshcheriakova A. A. , Dedysh S. N. 
Published: April 2024

Abstract—Planctomycetes are common inhabitants of northern wetland ecosystems. In this study, a new planctomycete of the genus Paludisphaera, strain Pla2T, was isolated from a boreal fen in Russia. The novel isolate was represented by nonmotile, pink-pigmented, spherical cells that multiplied by budding and occurred singly or were assembled in small aggregates. Strain Pla2T was a chemoorganotrophic, psychrotolerant mesophile with a growth optimum at pH 5.5‒6 and 15‒20°C. The preferred growth substrates were polysaccharides, including xylan, xanthan gum, and phytagel, as well as some sugars. The 16S rRNA gene sequence of strain Pla2T displayed the highest similarity (97.9%) to that of ‘Paludisphaera soli’ JC670T isolated from highland soil of the western Himalayas. With other members of the genus Paludisphaera, “P. rhizosphaerae” JC665T and P. borealis PX4T, this similarity was 97.0 and 93.8%, respectively. The genome of strain Pla2T was 8.21 Mb in size and contained about 6500 protein-coding genes and 3 copies of the rRNA operon. The DNA G + C content was 67 mol %. The average nucleotide identity between the genome sequence of strain Pla2T and those of previously described members of the genus Paludisphaera was between 79.4 and 82.6%. This genotypic distance as well as several phenotypic differences allowed classifying the new planctomycete from a fen as representing a novel species of the genus Paludisphaera, Paludisphaera mucosa sp. nov. with the type strain Pla2T (=KCTC92668T = VKM B-3698T).
<i>Ancylobacter crimeensis</i> sp. nov., a New Species of Aerobic Methylotrophic Bacteria Isolated from Oak Phyllosphere
Authors: Belova A. A. , Kaparullina E. N. , Agafonova N. V. , Grouzdev D. S. , Kopitsyn D. S. , Machulin А. V. , Doronina N. V. 
Published: April 2024

Abstract—A new facultative methylotroph, strain 6x-1T, was isolated from the phyllosphere of oak (Quercus pubescens Willd.) on the medium with methanol. Cells were aerobic, gram-negative, not-spore-forming, non-motile short ovoids rods reproducing by binary fission. Growth optimum was at 25‒29°C and pH 7.0‒7.5; growth was inhibited by 1.5% NaCl. Predominant fatty acids were C18:1ω7c and C19:0cyclo. Predominant phospholipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. Tha major ubiquinone was Q10. The 16S rRNA gene sequence of strain 6x-1T exhibited the highest similarity to those of members of the genus Ancylobacter (97.0‒97.4%). Genome analysis of strain 6x-1T and most closely related Ancylobacter strains revealed that the values of ANI (80.7‒83.5%), dDDH (22.4‒23.3%), AAI (72.0‒78.0%), and POCP (62.0‒69.0%) were below the recommended threshold values for prokaryotic species. Genome size of strain 6x-1T was 4.29 × 106 Mb, and G + C content was 67.3%. According to the results of phylogenetic, phylogenomic, phenotypic, and chemotaxonomic analysis, strain 6x-1T (=VKM В-3256T = ССUG 72401T) represents a new species of genus Ancylobacter, for which the name Ancylobacter crimeensis sp. nov. is proposed.
Polylactide Degradation in the Presence of Members of the Genus <i>Bacillus</i>
Authors: Mironov V. V. , Trofimchuk E. S. , Ostrikova V. V. , Plutalova A. V. , Moskvina M. A. , Shchelushkina A. A. , Chernikova E. V. , Sokolova D. S. 
Published: April 2024

Abstract—Microorganisms of the genus Bacillus were shown to have different effects on the degradation of polylactide packaging material. The degradation experiment was carried out on an agar medium at a temperature of 55°C and pH 5.9 for 14 days. This is the first report on the abiotic hydrolysis significantly slowing down during incubation with B. licheniformis S8 and occurring in parallel with the main process, enzymatic hydrolysis. The latter involved sequential cleavage of monomer units from the end of the macromolecule and the formation of low molecular weight products used by microorganisms as a substrate; it contributed to a decrease in the mass of polylactide by 5.1%, while maintaining its molecular weight and decreasing the dispersion of molecular weights. In the presence of bacteria B. amyloliquefaciens, B. subtilis subsp. spizizenii, and B. subtilis subsp. inaquosorum, the polymer weight did not decrease, but the molecular weight decreased significantly, similar to abiotic hydrolysis.
Occurrence of the Ability to Suppress the Growth of Related Strains in Rhizobia
Authors: Baymiev Al. Kh. , Vladimirova A. A. , Matniyazov R. T. , Lavina A. M. , Filyaeva K. Yu. , Akimova E. S. , Baymiev An. Kh. 
Published: April 2024

Abstract—Screening of 1019 strains of rhizobia Rhizobium leguminosarum and Neorhizobium galegae from the “Symbiont” collection of symbiotic rhizospheric microorganisms of the Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, which have been isolated from root nodules of 20 leguminous species, for the ability to suppress the growth of related strains of rhizobia was carried out. Inhibition of the growth of competing bacteria by rhizobia was shown to be rather common. On average, when grown on rich media, ~10% of rhizobial strains released antibacterial substances into the medium. At the same time, when a live culture of rhizobia was applied to the lawn of bacteria, the percentage of suppression was higher. The percentage of strains synthesizing antibacterial substances correlated also, to some extent, with the level of genetic diversity of the population of legume microsymbionts. A weak signal of impaired protein synthesis was found in the study of the mechanism of action of metabolites of 20 strains of rhizobia R. leguminosarum bv. viciae from root nodules of Lathyrus palustris and Lathyrus vernus. In other cases, the nature of the antibacterial action of bacterial metabolites could not be determined using the DualRep2 system.
<i>Methylomonas Montana </i>sp. nov., the First Unpigmented Methanotroph of the Genus <i>Methylomonas</i>, Isolated from Mountain River Sediments
Authors: Suleimanov R. Z. , Tikhonova E. N. , Oshkin I. Y. , Danilova O. V. , Dedysh S. N. 
Published: April 2024

Abstract—Aerobic methanotrophic bacteria of the genus Methylomonas inhabit a wide spectrum of habitats including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, unpigmented isolate of these bacteria, strain MW1T, which was obtained from sediments of the mountain river Khosta, Krasnodar region, Russia. Strain MW1T grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рН 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1T displayed 95.48–98.47% similarity to those in earlier described Methylomonas. The closest taxonomically characterized phylogenetic relative of strain MW1T was M. fluvii EbBT, isolated from the river Elbe sediments. Complete genome sequence of strain MW1T was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster pmoCAB coding for membrane methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1T with those in earlier described representatives of the genus Methylomonas was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus Methylomonas, M. montana sp. nov. Strain MW1T (=VKM 3737T = UQM 41536T) is the type strain of the newly proposed species.
LONG-TERM LIGHT DEPRIVATION AFFECTS DIGESTIVE FUNCTION IN RATS DURING ONTOGENY
Authors: Antonova E. P. , Morozov A. V. , Ilyukha V. A. , Khizhkin E. A. , Kalinina S. N. 
Published: April 2024

The effects of light deprivation on age-related changes in body weight, food and water intake, as well as the activity of digestive enzymes in pancreatic tissues of male rats was studied. Animals were divided into three groups: the first was in standard light conditions (12 h light/12 h dark, control, LD), the second was kept under conditions of long-term light deprivation from the moment of birth (LD/DD), and the third – from the prenatal period (DD/DD). Prolonged keeping of rats under conditions of light deprivation led to disruption of the age-associated dynamics of the studied parameters, at the same time, the detected changes in response to a specific light condition differed depending on the stage of ontogenesis at which its exposure began. Thus, body mass (age 3 and 18 months) and food and water intake (12 months), amylase and lipase activities (18 months) were lower, and protease activities after the end of the stable growth stage (12, 18 and 24 months) were higher in LD/DD-rats than in LD-rats. Significant changes in the studied parameters were observed under DD/DD conditions in 12‑month-old and 18-month-old rats – body mass was the largest, and food and water intake were the lowest compared to LD and LD/DD. In addition, the maximum amylase activity (1 and 6 months) among the studied groups and the higher values of total proteolytic activity during the period of stable growth (6 and 12 months) were found in DD/DD-rats compared to controls. Thus, our results indicate that light deprivation disrupts the ontogenetic development program of the digestive system of mammals.
IMMUNOPHENOTYPE OF MESENCHYMAL STEM CELLS DERIVED FROM EPICARDIAL AND PERIVASCULAR ADIPOSE TISSUE IN PATIENTS WITH CARDIOVASCULAR DISEASES
Authors: Uchasova E. G. , Dyleva Yu. A. , Belik E. V. , Matveeva V. G. , Gusev S. M. , Tarasova O. L. , Gruzdeva O. V. 
Published: April 2024

Aim: to evaluate the immunophenotype of adipose tissue stem cells isolated from epicardial and perivascular fat depots in patients with coronary heart disease and acquired heart defects. Results: In cell culture obtained from epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT) (2nd passage) in patients with both coronary heart disease and acquired heart defects, a high (over 90%) expression of membrane proteins characteristic of stem cells. In addition to the main population, with both in the EAT culture and in the PVAT, there were 2 minor ones: 1 – CD90-, CD105+, CD34-/+, CD73+, CD45- is presumably endothelial population, 2 – CD90+, CD105-, CD34-, CD73-, CD45- is the smallest population.
Conclusion: In the early stages of cultivation, cells of the stromal vascular fraction isolated from epicardial and perivascular adipose tissue express surface markers characteristic of adipose tissue stem cells.
TWO NEW SPECIES OF GELECHIID MOTHS OF THE GENUS <i>HELCYSTOGRAMMA</i> ZELLER 1877 (LEPIDOPTERA, GELECHIIDAE) FROM PANAMA
Authors: Omelko M. M. , Omelko Н. V. 
Published: April 2024

Two new species of the gelechiid moth genus Helcystogramma are described from Panama: H. jeffi sp. n. and H. almi sp. n. Based on male genitalia, they are assigned to the perelegans and trijunctum groups, respectively, both identified for the regional fauna of China. Helcystogramma effi sp. n. is morphologically similar to H. amethystias (Meyrick), from the Indo-Malay Region, and H. perelegans (N. Omelko et M. Omelko), from East Asia, but it differs by the uncus of the male genitalia bearing rounded lobes distally. Helcystogramma almi sp. n. in showing a variegate coloration of the wings is similar to H. carycastis (Meyrick), a species from Brazil and British Guiana, but in male genitalic structure it is closer to H. imagitrijunctum Li et Zhen and H. albilepidotum Li et Zhen, both from China.
THE POPULATION STRUCTURE OF THE SYMBIOTIC CRAB, <i>TRAPEZIA</i> <i>SEPTATA</i> (DECAPODA, TRAPEZIIDAE) AT THE EARLY STAGES OF CORAL COLONY DEVELOPMENT
Authors: Merkin V. A. , Britayev T. A. 
Published: April 2024

Symbiotic invertebrates play crucial roles in coral reef ecosystems due to their extremely high abundance and biomass, and by maintaining coral fitness and protecting them from predation. Among these invertebrates, symbiotic crabs of the family Trapeziidae are especially important for branching corals of the family Pocilloporidae. In this research, we studied the spatial arrangement and parameters of the populations of the symbiotic crab, Trapezia septata at the early stage of Pocillopora verrucosa colony formation. The study was conducted based on symbionts sampled from small coral colonies exposed for three months at a coral nursery in the Nha Trang Bay, Vietnam. As much as 59.5% of the study colonies are shown to be inhabited by the T. septata crab. The percentage of inhabited colonies grows proportionately to their volume. The male to female ratio is 1.4, being skewed to males. The number of males and females is increased with the volume of inhabited colonies, as is the average size of individuals. Individual colonies are commonly inhabited by a single crab or heterosexual pairs. Sexual dimorphism was found, females being larger than males. A positive relationship between the sizes of males and females in pairs was revealed, as in 73% pairs females were larger than males. The size of the clutch correlated positively to the size of the female. We hypothesize that the predominance of males in the population, as well as the individuals located among the hosts one by one, is the result of intersexual competition
A NEW SPECIES OF THE BRISTLETAIL GENUS <i>CTENOLEPISMA</i> (ZYGENTOMA, LEPISMATIDAE) FROM SOUTHWESTERN KAZAKHSTAN
Authors: Kaplin V. G. 
Published: April 2024

A new bristletail species is described from Kazakhstan: Ctenolepisma capsicum sp. n. It seems to be especially similar to such congeners common in Kazakhstan and Central Asia as C. mauritanicum (Lucas 1846), C. turkestanicum Kaplin Shakula 2022 and C. przewalsky Kaplin 1982, which all share a medium-sized and weakly to moderately pigmented body, two pairs of abdominal styli, the absence of bristle combs from abdominal sternites I and II, and of medial combs from all abdominal sternites; and urosternites III–VIII and the posterior edge of thoracic tergites each with 1 + 1 sublateral bristle combs. The first tergite of their abdomen bears 1 + 1 lateral, the second 2 + 2 or 3 + 3, terga III–VII each with 3 + 3, and tergite VIII with 2 + 2 bristle combs. These species differ well from each other in the number of sensory papillae on the apical article of the labial palps, the length ratios of the distal part of the prosternum occupied by bristle combs, and in the total length of the prosternum, the chaetotaxy of the second tergite of the abdomen, thoracic sternites, and the number of ovipositor articles.