bluuebunny/crossref_metadata_2025_split huggingface dataset:
Raloxifene and ICI182,780 Increase Estrogen Receptor-α Association with a Nuclear Compartment via Overlapping Sets of Hydrophobic Amino Acids in Activation Function 2 Helix 12
Authors: Lupien Mathieu , Jeyakumar M. , Hébert Elise , Hilmi Khalid , Cotnoir-White David , Loch Caroline , Auger Anick , Dayan Guila , Pinard Geneviève-Anne , Wurtz Jean-Marie , Moras Dino , Katzenellenbogen John , Mader Sylvie 
Published: February 2007

AbstractThe basis for the differential repressive effects of antiestrogens on transactivation by estrogen receptor-α (ERα) remains incompletely understood. Here, we show that the full antiestrogen ICI182,780 and, to a lesser extent, the selective ER modulator raloxifene (Ral), induce accumulation of exogenous ERα in a poorly soluble fraction in transiently transfected HepG2 or stably transfected MDA-MB231 cells and of endogenous receptor in MCF7 cells. ERα remained nuclear in HepG2 cells treated with either compound. Replacement of selected hydrophobic residues of ERα ligand-binding domain helix 12 (H12) enhanced receptor solubility in the presence of ICI182,780 or Ral. These mutations also increased transcriptional activity with Ral or ICI182,780 on reporter genes or on the endogenous estrogen target gene TFF1 in a manner requiring the integrity of the N-terminal AF-1 domain. The antiestrogen-specific effects of single mutations suggest that they affect receptor function by mechanisms other than a simple decrease in hydrophobicity of H12, possibly due to relief from local steric hindrance between these residues and the antiestrogen side chains. Fluorescence anisotropy experiments indicated an enhanced regional stabilization of mutant ligand-binding domains in the presence of antiestrogens. H12 mutations also prevent the increase in bioluminescence resonance energy transfer between ERα monomers induced by Ral or ICI182,780 and increase intranuclear receptor mobility in correlation with transcriptional activity in the presence of these antiestrogens. Our data indicate that ICI182,780 and Ral locally alter the ERα ligand binding structure via specific hydrophobic residues of H12 and decrease its transcriptional activity through tighter association with an insoluble nuclear structure.
Cold agglutinin induced autoimmune hemolytic anemia and NK‐cell leukemia—A new association
Authors: Skorupa Amy , Chaudhary Uzair B. , Lazarchick John 
Published: February 2007

AbstractCold agglutinin induced autoimmune hemolytic anemia is uncommonly associated with leukemia and lymphomas. We present a case of a young Mexican female presenting with a cold agglutinin hemolytic anemia with expression of a rare Pr antigen specificity and an aggressive NK‐cell leukemia. Our patient had a rapid fatal course. To our knowledge this is the first reported case of such an association. Am. J. Hematol., 2007. © 2007 Wiley‐Liss, Inc.
Inv (11)(p15q21) in donor‐derived Ph‐negative cells in a patient with chronic myeloid leukemia in relapse successfully treated with imatinib mesylate post allogeneic stemcell transplantation
Authors: Medeiros Bruno C. , Chun Kathy , Kamel‐Reid Suzanne , Lipton Jeffrey 
Published: February 2007

AbstractImatinib mesylate (IM) is the standard first‐line treatment for patients with chronic myeloid leukemia (CML). Surprisingly, 2–15% of patients achieving a complete cytogenetic response develop cytogenetic abnormalities in Philadelphia (Ph)‐negative cells. Following hematopoietic stem cell transplantation (HSCT), IM induces complete molecular responses (CMR) in ∼70% patients with relapsed CML, and no IM‐related cytogenetic abnormalities in Ph‐negative donor‐derived cells have been described after HSCT. We report a 56‐year‐old female who presented with a relapse from CML in September 2002. She had received a matched related HSCT for CML in chronic phase. Donor lymphocyte infusion was given 3 years post‐HSCT for a relapse. Sustained CMR was achieved within 3 months of initiation of IM. In October 2005, routine evaluation demonstrated continuous CMR, full male donor engraftment and an inv (11) on donor cells. Evaluation of the donor demonstrated no dysplasia or cytogenetic abnormalities. This observation reinforces the possibility that IM therapy may be casually linked to the phenomenon of secondary cytogenetic changes in diploid cells. Am. J. Hematol., 2007. © 2007 Wiley‐Liss, Inc.
Developmental changes in food transfers in cotton‐top tamarins (Saguinus oedipus)
Authors: Joyce Stella M. , Snowdon Charles T. 
Published: February 2007

AbstractWe investigated the development of food transfer and independent feeding in cotton‐top tamarin (Saguinus oedipus) families. We studied the relationship between infant‐directed vocalizations and food transfers on the development of independent feeding in infants. We experimentally tested ten infants (eight twins and two singletons) three times a week for 17 weeks from before weaning through 20 weeks. Food transfers and vocalizations made during tests were recorded and analyzed to determine (1) the role of vocalizations (C‐ and D‐ chirps and D‐chirp series) in food acquisition, (2) the relationship between food transfers and individual food acquisition, and (3) whether, owing to energetic costs of nursing and carrying twins, food is transferred to twins sooner than to singletons. Infants were more successful in acquiring food via begging when adults produced repeated vocalizations than when adults did not vocalize. Adults emitted more food‐related vocalizations in rapid series when in the presence of infants, whereas during feeding in the absence of infants only single unit vocalizations were produced. Begging occurred frequently. Changes over infant age were not significant when all infants were included in analysis. However, when twin data were analyzed alone, begging success changed significantly over months, with successful begging peaking in month 3 (week 12). Begging success rate did not differ between twins and singletons, although twins fed independently sooner and at a higher rate than did singletons. Am. J. Primatol. 69:955–965, 2007. © 2007 Wiley‐Liss, Inc.
Does smoking protect against osteoarthritis?
Authors: Felson David T. , Anderson Jennifer J. , Naimark Allan , Hannan Marian T. , Kannel William B. , Meenan Robert F. 
Published: February 2007

AbstractWhile studying knee osteoarthritis (OA) in the first Health and Nutrition Examination Survey, we unexpectedly found a protective association between smoking and OA. After adjustment for age, sex, and weight, smokers had a significantly lower rate of OA than did nonsmokers, and heavier smokers were less likely to have the disease than were light smokers. To test this association in a separate study and see if it was due to confounding factors, we looked at the Framingham Osteoarthritis Study, a study of elderly members of the Framingham Heart Study cohort. We evaluated whether the presence of knee OA in 1983–1985 was related to smoking status at the first Framingham examination, 36 years earlier. Subjects who had been smokers at examination 1 had a lower rate of OA (190 of 679, 28%) than did nonsmokers (276 of 736, 37.5%). In an analysis adjusted for age, sex, and weight, heavy smokers had a modestly lower risk of developing knee OA than did nonsmokers (relative risk 0.81). Also, the adjusted risk of severe OA was less in heavy smokers than in nonsmokers (relative risk 0.73). The negative association with OA persisted when we examined the average cigarette consumption over the first 10 years of the Framingham study. Furthermore, after controlling for age, sex, weight, knee injury history, sports activity history, physical activity level, coffee and alcohol consumption, and weight change after examination 1, and after modeling weight and age in a nonlinear manner, smoking remained a significant protector against later knee OA. It appears that smoking or some unidentified factor correlated with smoking modestly protects against the development of knee OA.
The “Neck Sign” in scleroderma
Authors: Barnett A. J. 
Published: February 2007

AbstractThe “neck sign” consists of ridging and tightening of the skin of the neck on extending the head. In this study it was found to be positive in over 90% of patients with scleroderma and negative in patients with primary Raynaud's disease and in control subjects. It is a useful diagnostic test for scleroderma.
Central nervous system vasculitis in Behçet's syndrome: Angiographic improvement after therapy with cytotoxic agents
Authors: Zelenski Jane D. , Calabrese Leonard H. , Capraro Judith A. , Holden Daniel 
Published: February 2007

AbstractWe describe a patient with Behçet's syndrome who had clinical and angiographic features of widespread cerebral angiitis of small‐ and medium‐caliber vessels. We report the successful treatment of this condition with a combination of cytotoxic agents and steroids, and demonstrate angiographically the resolution of these abnormalities in neuro‐Behçet's disease.
DNA–ANTI‐DNA Complexes account for part of the antihistone activity found in patients with systemic lupus erythematosus
Authors: Subiza J. L. , Caturla A. , Pascual‐Salcedo D. , Chamorro M. J. , Gazapo E. , Figueredo M. A. , de la Concha E. G. 
Published: February 2007

AbstractWe examined the effect of DNase treatment of sera with antihistone activity. In non–systemic lupus erythematosus (SLE) sera, antihistone levels remained unmodified, but a significant decrease was observed in 7 of 11 SLE sera with anti‐DNA antibodies. This was accompanied in some by an increase in anti‐DNA levels. We therefore considered that DNA–anti‐DNA complexes were being detected, as part of the antihistone activity in SLE patients, by binding of the complexes through their DNA to the histones used in the assay. This was confirmed by demonstrating that DNA–anti‐DNA complexes formed in vitro, and by studies performed with monoclonal antibodies with affinity to double‐stranded DNA and/or histones.
SPONTANEOUS PRODUCTION OF FIBRONECTIN BY ALVEOLAR MACROPHAGES IN PATIENTS WITH SCLERODERMA
Authors: Kinsella Margaret B. , Smith Edwin A. , Scott Miller K. , Leroy E. C. , Silver Richard M. 
Published: February 2007

AbstractInterstitial pulmonary fibrosis is a common manifestation of systemic sclerosis (SSc) and is a pathologic feature shared by a variety of other diseases. In these other disease processes, the glycoprotein fibronectin (FN) has been shown to be released by the alveolar macrophage, and is thus implicated in the development of fibrosis. We therefore studied the release of FN by alveolar macrophages obtained by bronchoalveolar lavage of 17 patients with SSc and 14 controls. We found that SSc alveolar macrophages released significantly more FN than did those of controls. Furthermore, the level of FN correlated positively with the level of inflammation determined by cellular analysis of lavage fluid and negatively with carbon monoxide diffusing capacity. FN may therefore play a role in the development of lung fibrosis in SSc and may be a marker of alveolitis.
Retardation of articular cartilage degradation by glycosaminoglycan polysulfate, pentosan polysulfate, and DH‐40J in the rat air pouch model
Authors: Francis D. J. , Forrest M. J. , Brooks P. M. , Ghosh P. 
Published: February 2007

AbstractThe rat subcutaneous air pouch model was adapted to examine the in vivo degradation of implanted rabbit articular cartilage, both with and without induced air pouch inflammation, over a 7‐day period. The effects of 3 drugs, glycosaminoglycan polysulfate (Arteparon), pentosan polysulfate (SP‐54), and zinc‐chelated pentosan polysulfate (DH‐40J), on inflammation‐induced cartilage degradation were also examined. Implanted articular cartilage from noninflamed air pouches showed a reduction in total proteoglycan (PG) content (as hexuronic acid), but not in PG extractability or aggregation, compared with cartilage maintained in tissue culture. The injection of peptone into the air pouch as an inflammogen caused an influx of leukocytes and plasma exudate and a reduction in implanted articular cartilage PG content, extractability, and aggregation, which was significantly greater than that which occurred in noninflamed air pouches. In vitro experiments demonstrated that peptone did not have a direct effect on cartilage PG degradation. Daily injection of Arteparon, SP‐54, or DH‐40J (10 mg/kg) into peptone‐inflamed air pouches significantly increased the PG content, extractability, and aggregation in implanted articular cartilage, compared with that in cartilage from non–drug‐treated control animals. The infiltration of leukocytes into the peptone‐inflamed air pouches was significantly reduced by daily administration of Arteparon, 10 mg/kg. At an equivalent dose, DH‐40J increased leukocyte numbers in the pouch fluid, whereas SP‐54 had no significant effect on leukocyte accumulation.
Retinyl acetate–induced arthritis in C3H‐Avy mice
Authors: Boden Scott D. , Labropoulos Panos A. , Ragsdal Bruce D. , Gullino Pietro M. , Gerber Lynn H. 
Published: February 2007

AbstractSeverely impaired musculoskeletal mobility in C3H‐Avy mice was noted during a pharmacologic trial evaluating the antitumorigenic properties of retinyl acetate (RAc). To determine the etiology of this impairment, we studied 103 female C3H‐Avy mice that were fed RAc in daily doses of 75–300 μg or placebo and were killed after 3–16 months. Whole‐body radiographs and histologic sections of the hindlimbs were scored for presence and severity of arthritis. C3H‐Avy mice treated with RAc in any dose had a significantly higher incidence of arthritis than placebo‐treated mice. Histologic evidence of enthesopathic disease closely paralleled the radiographic changes and ranged from small enthesophytes at tendinous and capsular insertions to complete periarticular bony bridging. Articular cartilage was not grossly affected. The incidence and severity of arthritis were significantly correlated with the total dose of RAc administered. The bony metaplasia induced by RAc was similar to the pathologic changes caused by other retinoids. This model may be useful for studying the pathogenesis of periarticular bone formation in diffuse idiopathic skeletal hyperostosis and related syndromes.
Elevated plasma levels of α2‐plasmin inhibitor–plasmin complex in patients with rheumatic diseases. Possible Role of Fibrinolytic Mechanism in Vasculitis
Authors: Kawakami Makoto , Kawagoe Mitsuhiro , Harigai Masayoshi , Hara Masako , Hirose Tatsuo , Hirose Wataru , Norioka Ken‐Ichi , Suzuki Kimihiro , Kitani Atsushi , Nakamura Haruo 
Published: February 2007

AbstractPlasma levels of α2‐plasmin inhibitor (α2PI) and α2‐plasmin inhibitor–plasmin complex (α2PIPC) were measured by sandwich enzyme‐linked immunosorbent assay, using a recently developed monoclonal anti‐α2PI antibody, in patients with collagen diseases. Twenty patients had systemic lupus erythematosus (SLE), 4 of whom also had vasculitis, 11 patients had rheumatoid arthritis (RA), 4 of whom also had vasculitis, and 5 patients had other types of vasculitis. There was no significant difference in α2PI levels between the 3 patient groups and the control groups. However, plasma levels of α2PIPC in all 3 patient groups were significantly higher than those in the control group. Moreover, plasma concentrations of α2PIPC in SLE patients with vasculitis were statistically significantly higher than those in SLE patients without vasculitis. These concentrations were also higher in RA patients with vasculitis than in RA patients without vasculitis, although the difference was not statistically significant. Our findings indicate that measurement of plasma α2PIPC levels is useful for detecting and evaluating the severity and activity of vasculitis in patients with collagen diseases.
Self‐report questionnaires in five rheumatic diseases comparisons of health status constructs and associations with formal education level
Authors: Callahan Leigh F. , Smith Wendy J. , Pincus Theodore 
Published: February 2007

AbstractSelf‐report questionnaire scales to assess various constructs of health status were compared in 602 patients with five rheumatic diseases, including 134 rheumatoid arthritis (RA), 216 osteoarthritis (OA), 84 fibromyalgia, 124 systemic lupus erythematosus (SLE), and 43 scleroderma patients. RA patients showed significantly higher degrees of difficulty, dissatisfaction, and pain in performing eight activities of daily living (ADL) compared to patients with the other four diseases (P < 0.01), while SLE patients reported the least difficulty, dissatisfaction and pain. Fibromyalgia patients showed significantly higher scores on a visual analog pain scale than patients with the other four diseases (P < 0.05), followed by OA patients. Fibromyalgia patients reported significantly higher levels of learned helplessness, assessed according to a rheumatology attitudes index (RAI), than patients with all other diseases, and scleroderma patients showed significantly lower RAI scores (P < 0.05). Patients with all five diseases who had not completed high school showed poorer clinical status than patients who had completed high school on all six scales. Significant differences in questionnaire scores were seen for 24 of 30 comparisons (five diseases and six scales) according to formal education level, versus only two according to age, and none according to duration of disease.
Antioxidant defense in mitochondria during diapause and postdiapause development of European corn borer (Ostrinia nubilalis, Hubn.)
Authors: Jovanović‐Galović A. , Blagojević D.P. , Grubor‐Lajšić G. , Worland M.R. , Spasić M.B. 
Published: February 2007

AbstractAntioxidant enzymes (CAT, catalase; GPx, selenium nondependent glutathione peroxidase; GST, glutathione‐S‐transferase; GR, glutathione reductase; DHAR, dehydroascorbate reductase) were determined in the mitochondria of diapausing and non‐diapausing larvae and pupae of both diapausing and non‐diapausing larvae of the European corn borer (Ostrinia nubilalis, Hubn., Lepidoptera: Pyralidae). CAT, GST, and DHAR activity in mitochondria of diapausing larvae were reduced compared to non‐diapausing larvae. Pupae of diapaused‐larvae possessed lower GST, but higher DHAR activities compared to pupae of non‐diapaused individuals. Comparison between larvae and pupae revealed lower GPx activity in the mitochondria of pupae. CAT activity in the mitochondria of pupae was higher compared to diapausing larvae, but lower than in non‐diapausing ones. Correlation and canonical discriminant analyses revealed different antioxidant enzyme compositions for a particular stage and developmental pattern. Our results show that antioxidant enzymes have a similar role in the regulation of energetics in mitochondria as that in diapause and metamorphosis. Arch. Insect Biochem. Physiol. 64:111–119, 2007. © 2007 Wiley‐Liss, Inc.
Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera
Authors: Nagalakshmi V.K. , Applebaum S.W. , Azrielli A. , Rafaeli A. 
Published: February 2007

AbstractInsect males produce accessory gland (MAG) factors that are transferred in the seminal fluid to females during copulation, and elicit changes in the mated female's behavior and physiology. Our previous studies showed that the injection of synthetic Drosophila melanogaster sex‐peptide (DrmSP) into virgin females of the moth Helicoverpa armigera causes a significant inhibition of pheromone production. In this and other moth species, pheromone production, correlated with female receptivity, is under neuroendocrine control due to the circadian release of the neuropeptide PBAN. In this study, we show that PBAN, present in the hemolymph during the scotophase in females, is drastically reduced after mating. We also identify 4 DrmSP‐like HPLC peaks (Peaks A, S1, S2, and B) in MAGs, with increasing levels of DrmSP immunoreactivity during the scotophase, when compared to their levels observed during the photophase. In H. armigera MAGs, a significant reduction in the pheromonostatic peak (Peak B) was already evident after 15 min of copulation, and depletion of an additional peak (Peak S2) was evident after complete mating. Peak A is also detected in female brains, increasing significantly 1 h after mating, at which time inhibition of pheromone biosynthesis also occurs. However, changes corresponding to the other MAG peaks were not detected in mated female tissues. Arch. Insect Biochem. Physiol. 64:142–155, 2007. © 2007 Wiley‐Liss, Inc.
Phenylbutazone toxicity: Depression of erythropoiesis
Authors: Swineford Oscar , Curry J. C. , Cumbia J. W. 
Published: February 2007

AbstractThe potential toxicity of phenylbutazone is again emphasized by a case of marked depression of erythropoiesis during the administration of this drug. Recovery apparently was hastened by treatment with prednisone.Le toxicitate potential de phenylbutazona es relevate de novo per un caso de depression marcate del erythropoiese durante le administration del droga. Le restablimento del patiente esseva accelerate, apparentemente, per tractamento con prednisona.
Familial occurrence of the rheumatoid factor
Authors: Ziff Morris , Schmid Frank R. , Lewis Arthur J. , Tanner Martin 
Published: February 2007

AbstractThe inhibition test and the latex fixation test (by the authors' modification) were both found positive more frequently in relatives of rheumatoid patients than in control groups. The authors, having assumed that a positive rheumatoid inhibition test, i.e., failure of sera to inhibit the sheep cell test (amboceptor), is due to the presence of small quantities of rheumatoid agglutinator in the euglobulin fraction, concluded from this and from the results of the capillary latex fixation procedure that increased amounts of rheumatoid factor are present in members of families of patients with rheumatoid arthritis.
“Rheumatoid” serologic reactions in experimental animals. II. Bentonite flocculation test in rats with experimental arthritis
Authors: Lerner Edwin M. , Bloch Kurt J. , Williams Robert R. 
Published: February 2007

AbstractRats with experimental infectious arthritis developed elevated BFT and SSCA titers. These reactions were also produced in immunized rats without joint lesions. Immunized rabbits developed high BFT titers only, and the responsible factor in rabbit serum was immunologically and physically distinct from human rheumatoid factor. The elevated BFT titers in infected or immunized animals appeared to be a response to human protein in the culture medium, but occurred only when the bacteria were actively growing in it.
Sex incidence of gonococcal arthritis
Authors: Graber William J. , Sanford Jay P. , Ziff Morris 
Published: February 2007

AbstractOf 48 cases of gonococcal arthritis seen between 1952 and 1958, 10, or 22 per cent, were males. The low incidence observed in males is in distinct contrast to the high incidence in this group reported prior to the antibiotic era. It is suggested that this change in sex incidence is the result of prompt treatment of the preceding symptomatic primary gonorrhea in the heterosexual male, whereas primary gonorrhea in the female is often unrecognized.
Stabilizers and inhibitors in the sensitized sheep‐cell agglutination reaction
Authors: Franklin Edward C. 
Published: February 2007

AbstractStudy of euglobulin fractions from normal sera has demonstrated two types of inhibitors for the sheep cell agglutination reaction. One of these is a gamma globulin, the other, an alpha‐2 globulin. Gamma globulin in an aggregated state neutralizes the rheumatoid factor by combining with it. The specific constitutent of the alpha‐2 globulins responsible for these properties is not yet known, nor has it been possible to define the mechanism by which these proteins stabilize the reaction. Certain properties of these two fractions are described and their possible roles in positive reactions in certain nonrheumatoid conditions are discussed.