bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlates of screening sigmoidoscopy use among men in a large nonprofit health plan
Authors: Haque Reina , Quinn Virginia P. , Habel Laurel A. , Enger Shelley M. , Sternfeld Barbara , Van Den Eeden Stephen K. , Sadler Marianne , Chiu Vicki , Caan Bette 
Published: June 2007

AbstractBACKGROUND.As the majority of patients diagnosed with colorectal cancer have no known risk factors, regular screening is strongly recommended. The authors examined factors associated with screening sigmoidoscopy use among participants in the California Men's Health Study (CMHS).METHODS.The authors conducted a cross‐sectional study over a 5‐year period nested within a prospective cohort study. The CMHS enrolled a large multiethnic cohort (n = 84,170) of men from 2 major California health plans. Because screening sigmoidoscopy was the preferred and most commonly used test for patients at average risk of colorectal cancer in the health plans, the authors excluded from the analysis men who completed a barium enema colonoscopy or a fecal occult blood test.RESULTS.Eligible subjects included 39,559 men at average risk for colorectal cancer. Prevalence of screening sigmoidoscopy use decreased with older age and increased with higher education and household income over the 5‐year study period. Compared with whites, Asians (adjusted OR, 1.42; 95% CI, 1.30–1.56) and African Americans (adjusted OR, 1.18; 95% CI, 1.08–1.29) were more likely to undergo screening sigmoidoscopy. Screening increased with the number of outpatient visits and with having a primary care provider in internal medicine. Men who did not undergo prostate‐specific antigen testing were also less likely to undergo sigmoidoscopy screening. Only 24.5% of current smokers had a screening sigmoidoscopy examination and were 25% less likely to undergo this procedure compared with nonsmokers (adjusted OR, 0.75; 95% CI, 0.69–0.82).CONCLUSIONS.In this insured population for whom financial barriers are minimized, screening sigmoidoscopy use was as low as reported in the general population. However, minority patients were not less likely to be screened. Cancer 2007. © 2007 American Cancer Society.
Novel tyrosine kinase inhibitor therapy before allogeneic stem cell transplantation in patients with chronic myeloid leukemia
Authors: Jabbour Elias , Cortes Jorge , Kantarjian Hagop , Giralt Sergio , Andersson Borje S. , Giles Francis , Shpall Elizabeth , Kebriaei Partow , Champlin Richard , de Lima Marcos 
Published: June 2007

AbstractBACKGROUND.Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) for chronic myeloid leukemia (CML) are increasingly likely to have received a novel tyrosine kinase inhibitor (NTKI) after failing imatinib mesylate. It is unknown whether the use of these NTKIs before HSCT increases transplant‐related toxicity.METHODS.The outcome of 12 patients with CML (1 in chronic phase, 6 in the accelerated phase, and 5 in the blastic phase) who received dasatinib (n = 2), nilotinib (n = 7), or both (n = 3) before HSCT were retrospectively analyzed.RESULTS.The median time on treatment was 134 days, and the median time from the end of NTKI therapy to HSCT was 34 days. The preparative regimen was ablative in 8 patients and nonablative in 4. All patients engrafted within 13 days. There was no significant early transplant‐related toxicity. One patient developed secondary graft failure after 6 months from the first HSCT that required a second HSCT. Acute and chronic graft‐versus‐host disease (GVHD) was observed in 7 and 6 patients, respectively. Nine patients achieved a molecular response: 4 complete and 5 major (quantitative reverse transcriptase‐polymerase chain reaction <0.05%). Three patients had disease progression by Day 30 after HSCT. Two patients developed disease recurrence after a median of 12 months. After a median follow‐up of 10 months, 7 patients were alive in molecular response and 5 patients had died, 4 of disease progression and 1 of extensive chronic GVHD.CONCLUSIONS.Previous treatment with NTKI did not increase transplant‐related toxicity in this preliminary experience. Further follow‐up and a larger number of patients will be necessary to confirm these observations. Cancer 2007. © 2007 American Cancer Society.
STAT1 activation in squamous cell cancer of the oral cavity
Authors: Laimer Klaus , Spizzo Gilbert , Obrist Peter , Gastl Guenther , Brunhuber Thomas , Schäfer Georg , Norer Burghard , Rasse Michael , Haffner Michael C. , Doppler Wolfgang 
Published: June 2007

AbstractBACKGROUND.For patients with squamous cell carcinoma of the oral cavity, both locoregional and distant recurrences are common, and an appropriate adjuvant treatment modality has yet to be defined. Thus, there is an urgent need to identify novel molecular markers with potential prognostic and/or predictive value to improve treatment outcome in these patients. This retrospective study was designed to investigate the predictive and/or prognostic value of STAT1 activation in squamous cell carcinoma of the oral cavity.METHODS.STAT1 expression and subcellular localization was examined immunohistochemically on a tissue microarray of paraffin‐embedded tumor specimens from 89 patients who underwent surgical treatment in the period between 1980 and 1997. A nuclear staining score of greater than 35% was defined as high STAT1 activation.RESULTS.According to study criteria, 18% of analyzed tumor samples exhibited high STAT1 activation. High STAT1 activation was associated with negative lymph node status. Moreover, in the subgroup of patients who received chemotherapy, high nuclear STAT1 staining in the tumor was associated with good prognosis.CONCLUSIONS.This is the first report demonstrating the potential predictive value of STAT1 activation status in patients with squamous cell cancer of the oral cavity. If confirmed in large prospective trials, this molecular marker could help in guiding therapeutic decisions in these patients. Cancer 2007. © 2007 American Cancer Society.
Simulation of motoneuron morphology in three dimensions. II. Building complete neurons
Authors: Marks William B. , Burke Robert E. 
Published: June 2007

AbstractBy using dendrogram data from six adult cat alpha motoneurons, we have constructed computer simulations of these cells in three dimensions (3D) by “growing” their dendritic trees from stem branches that were oriented as in the original cells. Individual trees were simulated by using the algorithms and parameters discussed in the companion paper (Marks and Burke [2007] J. Comp. Neurol. 503:685–700). It was not possible to distinguish real from simulated motoneurons by visual inspection of 3D drawings. Simulated cells were compared quantitatively with their actual exemplars by using features that were measured in spherical shells at various radii centered on the soma. These included nearest neighbor distances (NNDs) between branches, the sizes and overlaps between the territories of individual dendrites measured as convex hulls (polygons that enclose all branches passing through a shell), and the sizes of circular zones that contained no branches. We also compared the 3D fractal dimensions and lacunarity (a measure of the 3D dispersion of branches) in actual cells and their simulations. The statistical properties of these quantitative measures were not significantly different, suggesting that the simulation algorithm was quite successful. However, there were three exceptions: 1) there were more NNDs at distances < 50 μm in simulated than in actual motoneurons; 2) average overlaps between the territories of different dendrites were almost twice as large in simulated compared with actual motoneurons; and 3) estimates of lacunarity were also larger in simulated cells. These exceptions suggest that dendritic branches in actual motoneurons tend to avoid one another. We discuss possible interpretations of these results. J. Comp. Neurol. 503:701–716, 2007. Published 2007 Wiley‐Liss, Inc.
Real‐time fluorescence detection of multiple microscale cell culture analog devices in situ
Authors: Oh Taek‐il , Sung Jong Hwan , Tatosian Daniel A. , Shuler Michael L. , Kim Donghyun 
Published: June 2007

AbstractBackground:We investigated multiple microscale cell culture analog (μCCA) assays in situ with a high‐throughput imaging system that provides quantitative, nondestructive, and real‐time data on cell viability. Since samples do not move between measurements, captured images allow accurate time‐course measurements of cell population response and tracking the fate of each cell type on a quantitative basis. The optical system was evaluated by measuring the short‐term response to ethanol exposure and long‐term growth of drug‐resistant tumor cell lines with simultaneous samples.Methods:The optical system based on epi‐fluorescent excitation consists of an LED and a CCD as well as discrete optical components for imaging a large number of cells simultaneously. HepG2/C3A and MESSA cell lines were cultured in two μCCA systems for continuous cell status monitoring in cell death experiments with ethanol and long‐term cell growth.Results:The experiment that tested ethanol uptake showed that ethanol immediately caused cell death. The system was applied to extracting dynamic constants in the uptake process. In the long‐term cell growth experiment, growth of MESSA cells was followed by a stationary phase and eventual cell death attributed to nutrient and oxygen depletion and a change in the pH because of the accumulation of wastes by cell metabolism. HepG2/C3A cells were subject to contact inhibition and cell number did not change significantly over time. Issues related to long‐term assays are also discussed.Conclusions:The quantitative results have been consistent with qualitative images and confirm the applicability of the portable optical system, and potential application to high‐throughput analysis of cell‐based assays to measure long‐term dynamics. © 2007 International Society for Analytical Cytology
Analysis of plasma protein adsorption onto PEGylated nanoparticles by complementary methods: 2‐DE, CE and Protein Lab‐on‐chip® system
Authors: Kim Hyun Ryoung , Andrieux Karine , Delomenie Claudine , Chacun Héléne , Appel Martine , Desmaële Didier , Taran Fréderic , Georgin Dominique , Couvreur Patrick , Taverna Myriam 
Published: June 2007

AbstractThe biodistribution of colloidal carriers after their administration in vivo depends on the adsorption of some plasma proteins and apolipoproteins on their surface. Poly(methoxypolyethyleneglycol cyanoacrylate‐co‐hexadecylcyanoacrylate) (PEG‐PHDCA) nanoparticles have demonstrated their capacity to cross the blood–brain barrier (BBB) by a mechanism of endocytosis. In order to clarify this mechanism at the molecular level, proteins and especially apolipoproteins adsorbed at the surface of PEG‐PHDCA nanoparticles were analyzed by complementary methods such as CE and Protein Lab‐on‐chip® in comparison with 2‐D PAGE as a method of reference. Thus, the ability of those methodologies to identify and quantify human and rat plasma protein adsorption onto PEG‐PHDCA nanoparticles and conventional PHDCA nanoparticles was evaluated. The lower adsorption of proteins onto PEG‐PHDCA nanoparticles comparatively to PHDCA nanoparticles was evidenced by 2‐D PAGE and Protein Lab‐on‐chip® methods. CE allowed the quantification of adsorbed proteins without the requirement of a desorption procedure but failed, in this context, to analyze complex mixtures of proteins. The Protein Lab‐on‐chip® method appeared to be very useful to follow the kinetic of protein adsorption from serum onto nanoparticles; it was complementary to 2‐D PAGE which allowed the identification (with a relative quantification) of the adsorbed proteins. The overall results suggest the implication of the apolipoprotein E in the mechanism of passage of PEG‐PHDCA nanoparticles through the BBB.
The Biflow: An instrument for transfer‐loop mediated, continuous, preparative‐scale isoelectric trapping separations
Authors: Shave Evan , Vigh Gyula 
Published: June 2007

AbstractThe Biflow, a new isoelectric trapping instrument was designed to obtain a narrow ΔpI fraction from a complex feed in one step. The Biflow contains two identical separation units, each unit houses: an anode and cathode compartment, an anodic and cathodic membrane, an anodic and cathodic separation compartment, and a separation membrane. The separation units are connected to independent power supplies. The anodic membranes in Units 1 and 2 typically buffer at the same pH value and so do the cathodic membranes. The separation membranes in Units 1 and 2 buffer at different pH values, these determine the pI range (ΔpI) of the product. The cathodic separation compartments in Units 1 and 2 contain the feed and harvest streams. The two anodic separation compartments, connected through an electrically insulating air gap, form the transfer loop through which the transfer stream is recirculated between Units 1 and 2. Ampholytic components in the feed, with pI values lower than the pH of the buffering membrane in Unit 1, pass into the transfer stream and are shuttled into Unit 2. In Unit 2, components in the transfer stream which have pI values higher than the pH of the buffering membrane in Unit 2, pass into the harvest stream. This double transfer of the target component, oppositely directed, guarantees the complete exclusion of products outside the desired ΔpI range from the harvest stream. The utility of the Biflow unit was demonstrated by isolating carnosine from a mixture of UV‐absorbing ampholytes and ovalbumin isoforms as well as 4.4 <pI <4.5 and 5.4 <pI <5.5 minor constituents from chicken egg white.
Repeatability of chemical cytometry: 2‐DE analysis of single RAW 264.7 macrophage cells
Authors: Sobhani Kimia , Fink Susan L. , Cookson Brad T. , Dovichi Norman J. 
Published: June 2007

AbstractThis report presents the use of 2‐DE with ultrasensitive fluorescence detection as a chemical cytometry tool to characterize the protein and biogenic amine content of single cells from the RAW 264.7 murine macrophage cell line. Cells were sorted by cell cycle prior to 2‐DE analysis. Cells in the G2/M phase of the cell cycle were aspirated into the first‐dimensional capillary and lysed. The cellular contents were fluorescently labeled and first separated by capillary sieving electrophoresis (CSE). Over 380 fractions were transferred from the first‐dimensional capillary to the second‐dimensional capillary, where components were further separated by MEKC and detected by laser‐induced fluorescence. Twenty‐five spots common to the four electropherograms were fit with a 2‐D Gaussian surface to determine spot position, width, and amplitude. The RSD in CSE mobility was 1.0 ± 0.6%. The mean uncertainty in spot position was 1.3 times larger than the mean spot width in the CSE dimension. The average SD in MEKC migration time was 0.37 ± 0.13 s, which is smaller than the average spot size in this dimension. Spot capacity was 200. The RSD in spot amplitude was 50%, reflecting a large cell‐to‐cell variation in component expression.
Spatiotemporal analyses of interactions between entorhinal and CA1 projections to the subiculum in rat brain slices
Authors: Cappaert Natalie L.M. , Wadman Wytse J. , Witter Menno P. 
Published: June 2007

AbstractThe subiculum and the entorhinal cortex (EC) are important structures in processing and transmitting information between the neocortex and the hippocampus. The subiculum potentially receives information from the EC through two routes. In addition to a direct projection from EC to the subiculum, there is an indirect polysynaptic connection. The latter uses a number of possible pathways, which all converge onto the final projection from the hippocampal field CA1 to the subiculum. In this series of experiments we investigated to what extent activity in both pathways influences population activity of subicular neurons. We used voltage sensitive dyes in combined hippocampal‐EC slices of the rat to measure the spatio‐temporal activity patterns. To activate the two inputs to the subiculum, stimulation electrodes were placed in the stratum oriens/alveus of CA1 and in layer III of the medial EC. The response patterns evoked in the subiculum after electrical stimulation of each of these input pathways separately were compared with the response patterns after simultaneous stimulation of both areas (medial EC + CA1). A comparison of the computed added responses of the two individual stimulations with the measured responses after simultaneous stimulation suggests that both inputs are linearly added in the subiculum with very little nonlinear interactions. This strongly suggests that in the subiculum interaction at a single cell level of the direct and the indirect pathways from the EC is an unlikely scenario. © 2007 Wiley‐Liss, Inc.
Metal oxide coated cell culture arrays for rapid biological screening
Authors: Jarrell John D. , Eun Tai H. , Samale Marcus , Briant Clyde , Sheldon Brian W. , Morgan Jeffrey R. 
Published: June 2007

AbstractThe biointerface of metallic alloy implants is a spontaneously formed metal oxide layer. This study presents a novel method for creating titanium oxide xerogel coated microplates for high‐throughput biological screening that overcomes several limitations of using bulk metal samples to study oxides. Metal–organic precursors were used to evaluate the influence of Al, V, Ca, and P doped smooth and textured titanium oxide xerogel coatings on the bioresponse of human fibroblasts to increase understanding of the soft tissue sealing around transepithelial devices. Coatings made of titanium n‐butoxide were characteristically smooth, while those of titanium isopropoxide were micro‐ and nanofeatured. Screening consisted of WST‐1 proliferation assay, calcein AM cell number and viability assay, and a modified cell seeding efficiency and centrifugation adhesion assay. Small variations in initial attachment and centrifugation adhesion of human fibroblasts were observed among the coatings and comparable to tissue‐culture treated polystyrene. Proliferation and viability at 24 and 48 h were reduced by the 10 and 20% vanadium additions. Metal oxide coated microplates are adaptable to the investigation of a wide range of metal–organic derived chemistries and the influence of oxide texture, and level of oxide crystallinity and oxide grain size on the biological responses of cells. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Ring inversion in 1,4,7 cyclononatriene and analogues: Ab initio and DFT calculations and topological analysis
Authors: Zamora Miguel A. , Suvire Fernando D. , Enriz Ricardo D. 
Published: June 2007

AbstractThe multidimensional conformational potential energy hypersurfaces (PEHSs) for cis‐cis‐cis 1,4,7 cyclononatriene (I), Tribenzocyclononatriene (TBCN) (II), and cis‐cis‐cis cyclic triglycine (III) were comprehensively investigated at the Hartree–Fock (HF/6‐31G(d)) and density functional theory (B3LYP/6‐31G(d,p)) levels of theory. The equilibrium structures, their relative stability, and the transition state (TS) structures involved in the conformational interconversion pathways were analyzed. Altogether, four geometries (two low‐energy conformations and two transition states) were found to be important for a description of the conformational features of compounds I–III. B3LYP/aug‐cc‐pvdz//B3lYP/6‐31G(d,p) and MP2/6‐31G(d,p)//B3LYP/6‐31G(d,p) single point calculations predict that the conformational interconversion between crown and twist forms requires 14.01, 26.71, and 17.79 kcal/mol for compounds I, II, and III, respectively, which is in agreement with the available experimental data. A topological study of the conformational PEHSs of compounds I–III was performed. Our results allow us to form a concise idea about the internal intricacies of the PEHSs of compounds I–III, describing the conformations as well as the conformational interconversion process in these hypersurfaces. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008
Radiofrequency radiation (900 MHz) induces Egr‐1 gene expression and affects cell‐cycle control in human neuroblastoma cells
Authors: Buttiglione M. , Roca L. , Montemurno E. , Vitiello F. , Capozzi V. , Cibelli G. 
Published: June 2007

AbstractMany environmental signals, including ionizing radiation and UV rays, induce activation of Egr‐1 gene, thus affecting cell growth and apoptosis. The paucity and the controversial knowledge about the effect of electromagnetic fields (EMF) exposure of nerve cells prompted us to investigate the bioeffects of radiofrequency (RF) radiation on SH‐SY5Y neuroblastoma cells. The effect of a modulated RF field of 900 MHz, generated by a wire patch cell (WPC) antenna exposure system on Egr‐1 gene expression, was studied as a function of time. Short‐term exposures induced a transient increase in Egr‐1 mRNA level paralleled with activation of the MAPK subtypes ERK1/2 and SAPK/JNK. The effects of RF radiations on cell growth rate and apoptosis were also studied. Exposure to RF radiation had an anti‐proliferative activity in SH‐SY5Y cells with a significant effect observed at 24 h. RF radiation impaired cell cycle progression, reaching a significant G2‐M arrest. In addition, the appearance of the sub‐G1 peak, a hallmark of apoptosis, was highlighted after a 24‐h exposure, together with a significant decrease in mRNA levels of Bcl‐2 and survivin genes, both interfering with signaling between G2‐M arrest and apoptosis. Our results provide evidence that exposure to a 900 MHz‐modulated RF radiation affect both Egr‐1 gene expression and cell regulatory functions, involving apoptosis inhibitors like Bcl‐2 and survivin, thus providing important insights into a potentially broad mechanism for controlling in vitro cell viability. J. Cell. Physiol. 213:759–767. © 2007 Wiley‐Liss, Inc.
Matching cutsets in graphs
Authors: Moshi Augustine M. 
Published: June 2007

AbstractLet G = (V,E) be an undirected graph. A subset F of E is a matching cutset of G if no two edges of F are incident with the same point, and G‐F has more components than G. Chv́atal [2] proved that it is NP‐complete to recognize graphs with a matching cutset even if the input is restricted to graphs with maximum degree 4. We prove the following: (a) Every connected graph with maximum degree ⩽3 and on more than 7 points has a matching cutset. (In particular, there are precisely two connected cubic graphs without a matching cutset). (b) Line graphs with a matching cutset can be recognized in O(|E|) time. (c) Graphs without a chordless circuit of length 5 or more that have a matching cutset can be recognized in O(|V||E|3) time.
The 2‐hamiltonian cubes of graphs
Authors: Koh K. M. , Teo K. L. 
Published: June 2007

AbstractThis paper deals with the problem of characterizing the pairs of vertices x,y in a connected graph G such that G3 ‐ {x,y} is hamiltonian, where G3 is the cube of G. It is known that the cube G3 is 2‐hamiltonian if G is 2‐connected. In this paper, we first prove the stronger result that G3 ‐ {x,y} is hamiltonian if either x or y is not a cut‐vertex of G, and then proceed to characterize those cut‐vertices x and y of G such that G3 ‐{x,y} is hamiltonian. As a simple consequence of these, we obtain Schaar's characterization of a connected graph G such that G3 is 2‐hamiltonian.
Domination in graphs with minimum degree two
Authors: McCuaig William , Shepherd Bruce 
Published: June 2007

AbstractThe domination number γ(G) of a graph G = (V, E) is the minimum cardinality of a subset of V such that every vertex is either in the set or is adjacent to some vertex in the set. We show that if a connected graph G has minimum degree two and is not one of seven exceptional graphs, then γ(G)γ 2/5|V|. We also characterize those connected graphs with γ(G)γ 2/5|V|.
The ally‐reconstruction number of a tree with five or more vertices is three
Authors: Myrvold Wendy 
Published: June 2007

AbstractThe ally‐reconstruction number of a graph G, ally‐rn(G), is the minimum number of vertex‐deleted subgraphs required in order to identify G up to isomorphism. In this paper, we show that ally‐rn(T) = 3 for any tree T with five or more vertices.