bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clusters of adipose tissue dysfunction in adults with type 2 diabetes identify those with worse lipidomic profile despite similar glycaemic control
Authors: Della Pepa Giuseppe , Carli Fabrizia , Sabatini Silvia , Pezzica Samantha , Russo Marco , Vitale Marilena , Masulli Maria , Riccardi Gabriele , Rivellese Angela A. , Vaccaro Olga , Bozzetto Lutgarda , Gastaldelli Amalia 
Published: April 2024

AbstractAimsTo investigate clusters of adipose tissue dysfunction, that is, with adipose tissue insulin resistance (ADIPO‐IR) and large waist circumference (WC), identify a worse lipidomic profile characterised by a high proportion of lipids rich in saturated fatty acids (SFA).Materials and MethodsHierarchical clustering based on WC and ADIPO‐IR (calculated as fasting plasma non‐esterified fatty acids times fasting plasma insulin, FFA×INS), was performed in 192 adults with overweight/obesity and type 2 diabetes (T2D) treated with metformin (HbA1c = 7.8%). Free fatty acid composition and lipidomic profile were measured by mass spectrometry (GC‐MS and LC‐MSQTOF). Indexes of fatty acid desaturation (stearoyl‐coA desaturase‐1 activity, SCD116 = palmitoleic acid/palmitic acid and SCD118 = oleic acid/stearic acid) and of insulin resistance (HOMA‐IR) were also calculated.ResultsThree clusters were identified: CL1 (ADIPO‐IR = 4.9 ± 2.4 and WC = 96±7 cm, mean ± SD), CL2 (ADIPO‐IR = 6.5 ± 2.5 and WC = 114 ± 7 cm), and CL3 (ADIPO‐IR = 15.0 ± 4.7 and WC = 107 ± 8 cm). Insulin concentrations, ADIPO‐IR, and HOMA‐IR significantly increased from CL1 to CL3 (all p < 0.001), while fasting glucose concentrations, HbA1c, dietary lipids and caloric intake were similar. Moreover, CL3 showed significantly higher concentrations of monounsaturated free fatty acids, oleic and palmitoleic acids, triglycerides (TAG) rich in saturated FA and associated with de novo lipogenesis (i.e., TAG 46–50), higher SCD116, SCD118, ceramide (d18:0/18:0), and phosphatidylcholine aa(36:5) compared with CL1/CL2 (all p < 0.005).ConclusionsHigh ADIPO‐IR and large WC identify a worse lipid profile in T2D characterised by complex lipids rich in SFA, likely due to de novo synthesis given higher plasma monounsaturated FFA and increased desaturase activity indexes.Registration Number TrialID NCT00700856 https://clinicaltrials.gov.
Increased tree water use with the development of a dense understory layer in a North American hardwood forest
Authors: Rasoanaivo Arielle , Mekontchou Claudele Ghotsa , Rochon Pascal , Nolet Philippe , Maheu Audrey 
Published: April 2024

AbstractThe formation of a single species, recalcitrant understory vegetation layer can limit tree regeneration and, in the long term, modify the composition of forests. Few studies have investigated how recalcitrant vegetation influences competition for water resources although the formation of a dense understory is likely to modify the forest water balance. In eastern North American hardwood forests, the development of a dense understory layer of American beech (Fagus grandifolia) has been observed in stands dominated by sugar maple (Acer saccharum), a phenomenon that shares many characteristics associated with recalcitrant vegetation. Given that water availability is generally negatively correlated with stand density, we hypothesized that the formation of a dense understory beech layer increased competition for water resources, thus leading to reduced water use by sugar maple trees in beech‐dominated stands. Using thermal dissipation sensors, we measured sap flux density (Fd) of two sugar maple trees at three beech‐dominated sites and three control sites. During the growing season, Fd of sugar maple trees was significantly larger at beech‐dominated sites compared to control sites, indicating a greater rate of water use by sugar maples in stands with a dense understory beech layer. We provide two hypotheses to explain our results at the tree scale: (i) reduced cover by forest floor vegetation could limit transpiration by this layer, thus allowing increased water availability to supply transpiration by overstory trees, or (ii) increased tree transpiration rate could be a mechanism to satisfy nutrient requirements in beech‐dominated stands often associated with lower soil fertility.
Epithelial‐immune cell crosstalk for intestinal barrier homeostasis
Authors: Yao Yikun , Shang Wanjing , Bao Lingyu , Peng Zhaoyi , Wu Chuan 
Published: April 2024

AbstractThe intestinal barrier is mainly formed by a monolayer of epithelial cells, which forms a physical barrier to protect the gut tissues from external insults and provides a microenvironment for commensal bacteria to colonize while ensuring immune tolerance. Moreover, various immune cells are known to significantly contribute to intestinal barrier function by either directly interacting with epithelial cells or by producing immune mediators. Fulfilling this function of the gut barrier for mucosal homeostasis requires not only the intrinsic regulation of intestinal epithelial cells (IECs) but also constant communication with immune cells and gut microbes. The reciprocal interactions between IECs and immune cells modulate mucosal barrier integrity. Dysregulation of barrier function could lead to dysbiosis, inflammation, and tumorigenesis. In this overview, we provide an update on the characteristics and functions of IECs, and how they integrate their functions with tissue immune cells and gut microbiota to establish gut homeostasis.
High Precision Nail‐Penetration Setup for the Controlled Thermal Runaway Initiation of Lithium‐Ion Cells at Very Low Temperatures
Authors: Böttcher Nils , Dayani Shahabeddin , Markötter Henning , Bau Alexander , Setzchen Max , Schmidt Anita , Kowal Julia , Krug von Nidda Jonas 
Published: April 2024

A high precision nail‐penetration (NP) tool for characterizing the mechanically induced thermal‐runaway (TR) of lithium‐ion battery (LIB) cells in a defined range of temperatures down to −140 °C was developed. To understand the cell specific behavior at low temperatures aiming at the determination of safe handling conditions, different scenarios are analyzed. First, accuracy tests of the NP‐tool regarding motion and penetration depth are conducted with cylindrical cells at different temperatures. Thus, postmortem computer tomographic (CT) images are compared to the data measured with the newly integrated 3‐axis force sensor which is further combined with a high‐resolution position sensor. The herein developed setup allows evaluation of the NP‐metrics at an accuracy of ±1 pierced electrode layer without CT‐scans. Further NP examinations at 20 °C of fully charged cylindrical lithium nickel manganese cobalt oxide cells reveal a reproducible minimum damage as a reliable TR‐trigger. Moreover, NP‐tests at low temperature disclose a relation of the short circuit conductivity and TR‐reactions during subsequent rethermalization to room temperature. Finally, the implementation of a novel fixture for a controlled very fast cooling of LIB‐cells during critical damage opens the way to investigate the individual steps during a TR and, thus, to gain important information of the specific TR‐mechanism of different LIB‐cells.
Ictal kissing: Review of literature and report of 5 cases
Authors: Ravat Parthvi , Jain Neeraj , Thakkar Mayur , Kalika Mayuri , Ravat Sangeeta 
Published: April 2024

AbstractIctal kissing (IK) is a rare type of automatism observed during epileptic seizures. Despite its uncommon occurrence, understanding the underlying mechanisms, the role of emotions, and the level of consciousness during seizures with IK is essential in providing a comprehensive understanding of epilepsy. We describe five cases (.13%) of IK after performing a retrospective analysis of 3794 long‐term, ictal video‐EEGs from an epilepsy monitoring unit in Mumbai, India. Our patients with drug‐resistant epilepsy showed IK had a wide epileptogenic zone. We discuss the current hypotheses on the mechanisms behind IK, the involvement of temporal lobe structures, and the implications of awareness during seizures. The review concludes by suggesting future directions for research to elucidate the complex phenomenon of IK further.
Postoperative cognitive disorders and delirium in gynecologic surgery: Which surgery and anesthetic techniques to use to reduce the risk?
Authors: Pecorella Giovanni , De Rosa Filippo , Licchelli Martina , Panese Gaetano , Carugno Josè Tony , Morciano Andrea , Tinelli Andrea 
Published: April 2024

AbstractDespite their general good health, an increasing proportion of elderly individuals require surgery due to an increase in average lifespan. However, because of their increased vulnerability, these patients need to be handled carefully to make sure that surgery does not cause more harm than good. Age‐related postoperative cognitive disorders (POCD) and postoperative delirium (POD), two serious consequences that are marked by adverse neuropsychologic alterations after surgery, are particularly dangerous for the elderly. In the context of gynecologic procedures, POCD and POD are examined in this narrative review. The main question is how to limit the rates of POCD and POD in older women undergoing gynecologic procedures by maximizing the risk–benefit balance. Three crucial endpoints are considered: (1) surgical procedures to lower the rates of POCD and POD, (2) anesthetic techniques to lessen the occurrence and (3) the identification of individuals at high risk for post‐surgery cognitive impairments. Risks associated with laparoscopic gynecologic procedures include the Trendelenburg posture and CO2 exposure during pneumoperitoneum, despite statistical similarities in POD and POCD frequency between laparoscopic and laparotomy techniques. Numerous risk factors are associated with surgical interventions, such as blood loss, length of operation, and position holding, all of which reduce the chance of complications when they are minimized. In order to emphasize the essential role that anesthesia and surgery play in patient care, anesthesiologists are vital in making sure that anesthesia is given as sparingly and quickly as feasible. In addition, people who are genetically predisposed to POCD may be more susceptible to the disorder. The significance of a thorough strategy combining surgical and anesthetic concerns is highlighted in this article, in order to maximize results for senior patients having gynecologic surgery.
Subject matter changes in the dental curriculum: A scoping review of the last two decades
Authors: Rasmussen Emilie Leth , Musaeus Peter 
Published: April 2024

AbstractPurposeIn recent years, dental education has witnessed significant advancements and curriculum shifts in response to evolving healthcare needs, technological advances, and changing societal demands. As a result, dental universities worldwide have undergone substantial curriculum changes to ensure the adequate education and training of future dental professionals. The purpose of this study was to delineate the curriculum innovations over the past 20 years, with a focus on content within the current dental curriculum at universities worldwide.MethodThe articles reviewed for this manuscript were published from 2000 to June 2023. Search queries were conducted in four databases: Embase, PubMed, Web of Science, and ERIC, yielding 367 unique studies. Of these, 31 were included in the final synthesis.ResultsThe nine principal subject matter themes identified in the thematic analysis of these articles were critical scientific thinking, computational thinking, global mindset, geriatric dental care, interprofessional teamwork, transition to practice, local community, integration, and lifelong learning. The themes were modeled and mapped in a three‐axis figure, elucidating the interconnections between the themes.ConclusionThe identified themes signify the trajectory that dental education has taken and provide insights into the future course of the dental profession.
Microscopic investigation and pharmacognostic evaluation of leaves of Anemone rupicola Camb
Authors: Sultan Saba , Hussain Manzoor , Ashfaq Saleha , Ullah Aziz , Shabir Tahira , Khan Naqash , Husna Asma Ul 
Published: April 2024

AbstractThere is almost no scientific literature available on the phytochemistry and pharmacognosy of this plant so the basic aim of the study is to analyze pharmacognostic features of dried leaves of Anemone rupicola Camb. Morphology was studied by using the features like margins, shape, length, width, surface and so forth. For anatomical examination leaves were preserved in formalin acetic alcohol (FAA) and cutting the thin section of leaves and petiole with razor blade. Macroscopic characters of powdered drug were evaluated through organoleptic method. Qualitative phytochemical analysis was done by adopting the standard procedures. The antimicrobial activity of methanolic extract of Anemone rupicola Camb. was performed by Agar well diffusion method. The powder microscopic study showed epidermal cells, trichomes, various type of tracheids, pitted vessels, and sclerenchymatous fibers. Transverse cutting of leaves and petiole revealed the presence of different cells such as epidermis, palisade parenchyma, spongy parenchyma cells, aerenchyma, collenchyma, trichomes, and vascular bundles. The investigation of phytochemicals in distilled water, methanol and in ethanol solvent indicated the presence of various secondary compounds such as phenols, saponins, glycosides, alkaloids, tannins, cardiac glycosides, terpenoids, coumarins, and flavonoids. The methanolic extract of Anemone rupicola Camb. has potential as an antimicrobial agent. However, further studies are needed to confirm its efficacy and safety. This study can be used as powerful tool for the identification and authentication of this plant. For the purity and quality control these results will be helpful tool. This will also shed light into new areas where researchers can intervene in developing new drugs for future use.Research Highlights
Morphological Features of the Anemone rupicola
Anatomical examination of leaves of Anemeone rupicola
Microscopic features of powdered drug of leaves of Anemone rupicola
Qualitative Phytochemical analysis of the leaves of Anemone rupicola
Human transmission and outbreaks of feline‐like G6 rotavirus revealed with whole‐genome analysis of G6P[9] feline rotavirus
Authors: Fukuda Yuya , Kusuhara Hajime , Takai‐Todaka Reiko , Haga Kei , Katayama Kazuhiko , Tsugawa Takeshi 
Published: April 2024

AbstractGroup A rotaviruses (RVAs) are generally highly species‐specific; however, some strains infect across species. Feline RVAs sporadically infect humans, causing gastroenteritis. In 2012 and 2013, rectal swab samples were collected from 61 asymptomatic shelter cats at a public health center in Mie Prefecture, Japan, to investigate the presence of RVA and any association with human infections. The analysis identified G6P[9] strains in three cats and G3P[9] strains in two cats, although no feline RVA sequence data were available for the former. A whole‐genome analysis of these G6P[9] strains identified the genotype constellation G6‐P[9]‐I2‐R2‐C2‐M2‐A3‐N2‐T3‐E3‐H3. The nucleotide identity among these G6P[9] strains exceeded 99.5% across all 11 gene segments, indicating the circulation of this G6P[9] strain among cats. Notably, strain RVA/Human‐wt/JPN/KF17/2010/G6P[9], previously detected in a 3‐year‐old child with gastroenteritis, shares high nucleotide identity (>98%) with Mie20120017f, the representative G6P[9] strain in this study, across all 11 gene segments, confirming feline RVA infection and symptomatic presentation in this child. The VP7 gene of strain Mie20120017f also shares high nucleotide identity with other sporadically reported G6 RVA strains in humans. This suggests that feline‐origin G6 strains as the probable source of these sporadic G6 RVA strains causing gastroenteritis in humans globally. Moreover, a feline‐like human G6P[8] strain circulating in Brazil in 2022 was identified, emphasizing the importance of ongoing surveillance to monitor potential global human outbreaks of RVA.
DC‐bias and temperature included CSWPL model for RF power transistors
Authors: Liu Enduo , Tang Xiaoqiang , Crupi Giovanni , Cai Jialin 
Published: April 2024

AbstractA novel frequency domain behavioral modeling method for gallium‐nitride (GaN) devices is presented in this article. By utilizing a multi‐dimensional polynomial function, the proposed technique interpolates DC‐bias voltage and temperature based on the Canonical section‐wise piecewise linear (CSWPL) model framework. A detailed description of the model's theory is provided. With data from 10‐W GaN devices, the model was implemented in commercial software and validated through both DC and radio frequency (RF) tests. A robust predictive ability is demonstrated by the obtained results, thus proving the accuracy of the developed modeling method. This model is superior to the standard CSWPL model in that it is capable of predicting transistor behavior at different bias voltages and temperatures using a single set of parameters, thereby greatly reducing the complexity of the model and the time required for extraction.
Pepper vein yellow virus P0 protein triggers NbHERC3, NbBax, and NbCRR mediated hypersensitive response
Authors: OuYang Xian , Wang Lishuang , Luo Xiangwen , Li Chun , An Xingyu , Yao Ling , Huang Wei , Zhang Zhanhong , Zhang Songbai , Liu Yong , Wu Shiping 
Published: April 2024

AbstractP0 proteins encoded by the pepper vein yellow virus (PeVYV) are pathogenic factors that cause hypersensitive response (HR). However, the host gene expression related to PeVYV P0‐induced HR has not been thoroughly studied. Transcriptomic technology was used to investigate the host pathways mediated by the PeVYV P0 protein to explore the molecular mechanisms underlying its function. We found 12,638 differentially expressed genes (DEGs); 6784 and 5854 genes were significantly upregulated and downregulated, respectively. Transcriptomic and reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR) analyses revealed that salicylic acid (SA) and jasmonic acid (JA) synthesis‐related gene expression was upregulated, and ethylene synthesis‐related gene expression was downregulated. Ultrahigh performance liquid chromatography‐tandem mass spectrometry was used to quantify SA and JA concentrations in Nicotiana benthamiana, and the P0 protein induced SA and JA biosynthesis. We then hypothesized that the pathogenic activity of the P0 protein might be owing to proteins related to host hormones in the SA and JA pathways, modulating host resistance at different times. Viral gene silencing suppression technology was used in N. benthamiana to characterize candidate proteins, and downregulating NbHERC3 (Homologous to E6‐AP carboxy‐terminus domain and regulator of choromosome condensation‐1 dmain protein 3) accelerated cell necrosis in the host. The downregulation of NbCRR reduced cell death, while that of NbBax induced necrosis and curled heart leaves. Our findings indicate that NbHERC3, NbBax, and NbCRR are involved in P0 protein‐driven cell necrosis.
Bone‐patellar tendon‐bone autograft is associated with a higher rate of return to preinjury levels of performance in high‐level athletes than anterior cruciate ligament reconstruction using hamstring autograft
Authors: Tomihara Tomohiro , Hashimoto Yusuke , Okazaki Shiro , Nishino Kazuya , Taniuchi Masatoshi , Takigami Junsei , Tsumoto Shuko , Katsuda Hiroshi 
Published: April 2024

AbstractPurposeReturn to preinjury levels of performance (RTP) is the main goal after anterior cruciate ligament reconstruction (ACL‐R) for athletes when ACL graft rupture is a career‐threatening event. The purpose of this study was to elucidate the associated factors for RTP and subsequent ACL injury after ACL‐R using bone‐patellar tendon‐bone (BPTB) or hamstring (HT) autograft in high‐level athletes with a minimum postoperative follow‐up of 24 months.MethodsThis retrospective study included 157 patients who had preinjury Tegner activity level of 9 and underwent primary ACL‐R using BPTB (average age, 16.9 years; 35 males and 36 females) or HT (average age, 17.2 years; 49 males and 37 females). The mean follow‐ups were 33.6 months in BPTB and 44.5 months in HT, respectively. The data were obtained based on routine clinical follow‐ups and telephone interviews performed by the surgeon. Multivariate logistic regression analysis was conducted to determine the association of patient variables with RTP and subsequent ACL injury.ResultsNinety‐nine patients (63.1%) were able to RTP. The rate of RTP in BPTB (74.6%) was significantly higher than that of HT (53.5%) (p < 0.05). The overall average timing of RTP after ACL‐R was 10.0 months while that was significantly earlier in BPTB (9.7 months) than in HT (10.5 months) (p < 0.05). Twenty‐three (14.6%) and 21 patients (13.4%) had ACL graft ruptures and ACL injuries in the contralateral knees, respectively. Multivariate analyses showed that BPTB (odds ratio [OR], 2.590; 95% confidence interval [CI], 1.300–5.160; p = 0.007) was associated with a higher potential for RTP after ACL‐R. The incidence of ACL graft rupture after ACL‐R decreased with BPTB (OR, 0.861; 95% CI, 0.770–0.962; p = 0.009).ConclusionsThe use of BPTB autograft was associated with a higher rate of RTP and a lower incidence of ACL graft rupture compared to ACL‐R using HT autograft.Level of EvidenceLevel IV, retrospective case series.
Population‐based study of environmental heavy metal exposure and hearing loss: A systematic review and meta‐analysis
Authors: Wang Fang , Böhnke Frank , Böck Katja , Wirth Markus 
Published: April 2024

AbstractBackground and ObjectivesPrevious studies have shown an association between environmental exposure to heavy metals and hearing loss. However, the findings regarding the relationship between exposure to different metals and hearing loss development are inconsistent. To address this, we conducted a meta‐analysis to explore the link between common heavy metal exposures and hearing loss. This study examined the effects of lead (Pb), cadmium (Cd), and mercury (Hg) pollution on hearing loss at various levels, and systematically reviewed the literature on manganese (Mn), barium (Ba), arsenic (As), and hearing loss.MethodsWe conducted systematic searches in five major databases, including PubMed, Web of Science, Embase, Cochrane Library, and Scopus. In addition, we searched three Chinese digital libraries: CNKI, Wanfang Data, and Wipu. From an initial pool of 649 articles, we carefully screened and selected 15 articles for further analysis. The effect sizes from these selected studies were synthesized through a meta‐analysis to calculate the overall effect size.ResultsOur findings showed that: (1) There was a significant association between Pb and Cd exposure and hearing loss; (2) There is a proportional relationship between the increase of metal index detected in blood and hearing loss; (3) In the PTA measurement of hearing loss at different frequencies, the 4 kHz high frequency range had a stronger correlation with hearing loss than the low frequency, with OR 1.44 (1.22, 1.71); and (4) There was a more significant correlation between Barium (Ba) levels in nails and hair than in urine.ConclusionsThe study presented evidence of a significant association between human hearing loss and exposure to lead (Pb) and cadmium (Cd). It not only revealed a positive correlation between blood heavy metal concentrations and the incidence of hearing loss but also highlighted that long‐term exposure indicators of heavy metals were more indicative of the correlation with hearing loss. Lastly, the study recommends utilizing high frequency 4 kHz for the effective assessment and diagnosis of hearing loss caused by exposure to heavy metals.