bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility of Robot-Assisted Cytoreductive Surgery With Upper-Abdominal Peritonectomy for Pseudomyxoma Peritonei With Low Peritoneal Carcinomatosis Index: A Pilot Study
Authors: Fujimoto Daisuke , Yonemura Yutaka , Taniguchi Keizo , Kobayashi Hirotoshi 
Published: February 2024

Introduction:
Our study’s objective was to provide the method for, and preliminary findings from, robot-assisted cytoreductive surgery (r-CRS) combined with upper-abdominal peritonectomy in pseudomyxoma peritonei (PMP) with limited peritoneal surface malignancy (PSM).

Materials and Methods:
We conducted a retrospective pilot study on consecutive patients with PSM secondary to pseudomyxoma peritonei with a peritoneal cancer index (PCI) of under 10 who were indicated for r-CRS combined with upper-abdominal peritectomy. Perioperative and 30-day major morbidity and mortality characteristics were analyzed and compared with cases in which laparoscopic CRS (l-CRS) was performed under the same conditions.

Results:
Six patients underwent r-CRS combined with an upper-abdominal partial peritonectomy. Their mean PCI was 4.83. Complete cytoreduction was achieved in all patients. The mean duration of the operation was 156.8 minutes. There was no major complication and no mortality. The mean length of hospital stay after surgery was 6.33 days. r-CRS resulted in less blood loss, a lower C-reactive protein level, and a shorter length of hospital stay that were significantly different compared with those of l-CRS.

Conclusions:
This is the initial technical report of a robotic approach for CRS combined with upper-abdominal peritonectomy. r-CRS with a combined upper-abdominal peritonectomy was shown to be safe and feasible for PMP with limited PSM.
Programming quadric metasurfaces via infinitesimal origami maps of monohedral hexagonal tessellations: Part II
Authors: dos Santos Filipe A. , Favata Antonino , Micheletti Andrea , Paroni Roberto , Picchi Scardaoni Marco 
Published: February 2024

In Part I of this study, it was shown that all the three known types of monohedral hexagonal tessellations of the plane, those composed of equal irregular hexagons, have just a single deformation mode when tiles are considered as rigid bodies hinged to each other along the edges. A gallery of tessellated plates was simulated numerically to demonstrate the range of achievable deformed shapes. In Part II, the displacement field was first derived and a continuous interpolant for each type of tessellated plate. It turns out that all corresponding metasurfaces are described by quadrics. Afterwards, a parametric analysis was carried out to determine the effect of varying angles and edge lengths on the curvature, and the values of the geometric Poisson ratio of the plates. Finally, a method of fabrication is proposed based on the additive manufacturing of stiff tiles of negligible deformability and flexible connectors. Using this modular technique, it is possible to join together different monohedral tessellated plates able to deform into piece-wise quadrics. The nodal positions in the deformed configuration of the realized plates are measured after enforcing one principal curvature to assume a chosen value. The estimate of the other principal curvature confirms the analytical predictions. The presented tessellated plates permit to realize doubly curved shape-morphing metasurfaces with assorted shapes, which also can feature a certain surface roughness, and they can be employed in all applications demanding high surface accuracy and few actuators or just one.
Roseibium algicola sp. nov. and Roseibium porphyridii sp. nov., isolated from marine red algae
Authors: Lee Min Woo , Kim Jeong Min , Kim Kyung Hyun , Choi Dae Gyu , Lee Jae Kyeong , Baek Ju Hye , Jeon Che Ok 
Published: February 2024

Two Gram-stain-negative, strictly aerobic rods, designated as RMAR6-6T and KMA01T, exhibiting catalase- and oxidase-positive activities, were isolated from marine red algae in the Republic of Korea. Cells of strain RMAR6-6T exhibited flagellar motility, while those of strain KMA01T were non-motile. Strain RMAR6-6T exhibited optimal growth at 30–35°C and pH 7.0–8.0 with 4.0–6.0 % (w/v) NaCl, while strain KMA01T grew optimally at 30–35 °C, pH 7.0–8.0 and 2.0–5.0% NaCl. Both strains shared common major respiratory isoprenoid quinone (ubiquinone-10), cellular fatty acids (C18 : 0, C18: 1

ω7c 11-methyl, C20 : 1
ω7c and summed feature 8) and polar lipids (phosphatidylglycerol, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sulphoquinovosyldiacylglycerol). The genomic DNA G+C contents were 59.0 and 55.0 mol% for strains RMAR6-6T and KMA01T, respectively. With 98.5 % 16S rRNA gene similarity, 75.2 % average nucleotide identity (ANI) and 19.8 % digital DNA–DNA hybridization (dDDH) values, strains RMAR6-6T and KMA01T were identified as representing distinct species. Phylogenetic analyses based on both 16S rRNA gene and genome sequences revealed that strains RMAR6-6T and KMA01T formed distinct phylogenic lineages within the genus Roseibium, most closely related to Roseibium aggregatum IAM 12614T and Roseibium album CECT 5094T, respectively. The ANI and dDDH values between strain RMAR6-6T and R. aggregatum IAM 12614T were 87.5 and 33.3 %, respectively. Similarly, the values between KMA01T and R. album CECT 5094T were 74.2 % (ANI) and 19.3 % (dDDH). Based on phenotypic, chemotaxonomic and molecular characteristics, strains RMAR6-6T and KMA01T represent two novel species of the genus Roseibium, for which the names R. algicola sp. nov. (RMAR6-6T=KACC 22482T=JCM 34977T) and R. porphyridii sp. nov. (KMA01T=KACC 22479T=JCM 34597T) are proposed, respectively.
Relapse after intermittent access to cocaine: Discriminative cues more effectively trigger drug seeking than do conditioned cues
Authors: Ndiaye Ndeye Aissatou , Shamleh Sema Abu , Casale Domiziana , Castaneda-Ouellet Sol’Abraham , Laplante Isabel , Robinson Mike J. F. , Samaha Anne-Noël 
Published: February 2024

AbstractWhen people with drug addiction encounter cues associated with drug use, this can trigger cravings and relapse. These cues can include conditioned stimuli (CSs) signaling drug delivery and discriminative stimuli (DSs) signaling drug availability. Compared to CS effects, DS effects are less explored in preclinical studies on cue-induced relapse. We compared CS and DS effects on reward seeking following abstinence from intermittent-access cocaine (or sucrose) self-administration. During 15-20 intermittent-access sessions, rats self-administered sucrose pellets or i.v. cocaine paired with a light-tone CS. Cocaine/sucrose was available for 5-min (signalled by a light; DS+) and unavailable for 25 min (signalled by different lighting conditions; DS-), and this cycled for 4 h/session. Following abstinence, we measured cocaine/sucrose seeking under extinction triggered by CS and DS presentation, and instrumental responding reinforced by these cues. Across intermittent-access sessions, rats increased lever pressing for cocaine or sucrose during DS+ periods and decreased responding during DS-periods. On days 2 and 21 of abstinence, only presentation of the DS+ or DS+ and CS combined elicited increased cocaine/sucrose-seeking behaviour (i.e., increased active lever presses). Additionally, presenting the DS-alongside the DS+ suppressed the increased cocaine-seeking behaviour otherwise produced by the DS+. Finally, on day 21 of abstinence, rats showed equivalent levels of lever pressing reinforced by the DS+, CS and by the DS+ and CS combined, suggesting comparable conditioned reinforcing value. After intermittent self-administration, cocaine-associated DSs and CSs acquire similar conditioned reinforcing properties, but DSs more effectively trigger increases in drug seeking.
Resilience and vulnerability of neural speech tracking in children with cochlear implants
Authors: Federici Alessandra , Fantoni Marta , Pavani Francesco , Handjaras Giacomo , Bednaya Evgenia , Martinelli Alice , Berto Martina , Ricciardi Emiliano , Nava Elena , Orzan Eva , Bianchi Benedetta , Bottari Davide 
Published: February 2024

AbstractInfants are born with biological biases that favour language acquisition. One is the auditory system’s ability to track the envelope of continuous speech, a pivotal feature for spoken language comprehension in adulthood. However, the extent to which neural speech tracking relies on postnatal auditory experience remains unknown. In this case-control study, we tested children with or without access to functional hearing in the first year of life after they received cochlear implants (CIs) for hearing restoration. We measured neural speech tracking in CI users with a congenital bilateral profound deafness (CD) or who acquired it later in development (AD; minimum auditory experience after birth 12 months), as well as in two groups of hearing controls listening to original (HC) or vocoded-speech (HC-v). Remarkably, neural speech tracking in children with CIs was unaffected by the absence of perinatal auditory experience. Regardless of deafness onset, CI users and HC exhibited a similar neural tracking magnitude at short timescales ∼50– 130 ms (P1TRF) of brain activity. However, this neural tracking phase (P1TRF) was delayed in CI users, and its timing depended on the age of hearing restoration. Conversely, at longer timescales ∼130–260 ms (N2TRF) of brain activity, speech tracking was substantially dampened in participants with CIs, thereby accounting for their comprehension deficits. Speech tracking in HC listening to vocoded-speech and in a phantom head-model with CIs suggested that neural processing differences between HC and CI children could not merely be explained by the degraded acoustic stimulation, nor by electrical artifacts of the implants. These findings highlight (i) the resilience of sensory components of neural speech tracking to the lack of hearing in the first year of life, (ii) the crucial role played when hearing restoration takes place in mitigating the impact of atypical auditory experience, (iii) the vulnerability of higher hierarchical levels of speech processing in CI users.
Subjective confidence modulates individual BOLD patterns of predictive processing
Authors: Hechler André , de Lange Floris P. , Riedl Valentin 
Published: February 2024

AbstractHumans are adept at extracting and learning sequential patterns from sensory input. This ability enables predictions about future states, resulting in anticipation both on a behavioral and neural level. Stimuli deviating from predictions usually evoke higher neural and hemodynamic activity than predicted stimuli. This difference indicates increased surprise, or prediction error signaling in the context of predictive coding. However, interindividual differences in learning performance and uncertainty have rarely been taken into account. Under Bayesian formulations of cortical function, surprise should be strongest if a subject makes incorrect predictions with high confidence.In the present study, we studied the impact of subjective confidence on imaging markers of predictive processing. Participants viewed visual object sequences of varying predictability over multiple days. After each day, we instructed them to complete partially presented sequences and to rate their confidence in the decision. During fMRI scanning, participants saw sequences that either confirmed predictions, deviated from them, or were random. We replicated findings of increased BOLD responses to surprising input in the ventral visual stream. In line with our hypothesis, response magnitude increased with the level of confidence after the training phase. Interestingly, the activity difference between predictable and random input also scaled with confidence: In the anterior cingulate, we found tentative evidence that predictable sequences elicited higher activity for low levels of confidence, but lower activity for high levels of confidence. In summary, we showed that confidence is a crucial moderator of the link between predictive processing and BOLD activity.
Multimodal histopathologic models stratify hormone receptor-positive early breast cancer
Authors: Boehm Kevin M. , El Nahhas Omar S. M. , Marra Antonio , Selenica Pier , Wen Hannah Y. , Weigelt Britta , Paul Evan D. , Cekan Pavol , Erber Ramona , Loeffler Chiara M. L. , Guerini-Rocco Elena , Fusco Nicola , Frascarelli Chiara , Mane Eltjona , Munzone Elisabetta , Dellapasqua Silvia , Zagami Paola , Curigliano Giuseppe , Razavi Pedram , Reis-Filho Jorge S. , Pareja Fresia , Chandarlapaty Sarat , Shah Sohrab P. , Kather Jakob Nikolas 
Published: February 2024

AbstractFor patients with hormone receptor-positive, early breast cancer withoutHER2amplification, multigene expression assays including Oncotype DX ® recurrence score (RS) have been clinically validated to identify patients who stand to derive added benefit from adjuvant cytotoxic chemotherapy. However, cost and turnaround time have limited its global adoption despite recommendation by practice guidelines. We investigated if routinely available hematoxylin and eosin (H&E)-stained pathology slides could act as a surrogate triaging data substrate by predicting RS using machine learning methods. We trained and validated a multimodal transformer model, Orpheus, using 6,203 patients across three independent cohorts, taking both H&E images and their corresponding synoptic text reports as input. We showed accurate inference of recurrence score from whole-slide images (r = 0.63 (95% C.I. 0.58 - 0.68); n = 1,029), the raw text of their corresponding reports (r = 0.58 (95% C.I. 0.51 - 0.64); n = 972), and their combination (r = 0.68 (95% C.I. 0.64 - 0.73); n = 964) as measured by Pearson’s correlation. To predict high-risk disease (RS>25), our model achieved an area under the receiver operating characteristic curve (AUROC) of 0.89 (95% C.I. 0.83 - 0.94), and area under the precision recall curve (AUPRC) of 0.64 (95% C.I. 0.60 - 0.82), compared to 0.49 (95% C.I. 0.36 - 0.64) for an existing nomogram based on clinical and pathologic features. Moreover, our model generalizes well to external international cohorts, effectively identifying recurrence risk (r = 0.61,p< 10-4, n = 452; r = 0.60,p< 10-4, n = 575) and high-risk status (AUROC = 0.80,p< 10-4, AUPRC = 0.68,p< 10-4, n = 452; AUROC = 0.83,p< 10-4, AUPRC = 0.73,p< 10-4, n = 575) from whole-slide images. Probing the biologic underpinnings of the model decisions uncovered tumor cell size heterogeneity, immune cell infiltration, a proliferative transcription program, and stromal fraction as correlates of higher-risk predictions. We conclude that at an operating point of 94.4% precision and 33.3% recall, this model could help increase global adoption and shorten lag between resection and adjuvant therapy.
Schlafen-11 and -9 are innate immune sensors for intracellular single-stranded DNA
Authors: Zhang Peng , Hu Xiaoqing , Li Zekun , Liu Qian , Liu Lele , Jin Yingying , Liu Sizhe , Zhao Xiang , Wang Jianqiao , Hao Delong , Chen Houzao , Liu Depei 
Published: February 2024

AbstractNucleic acids are major structures detected by the innate immune system. Although intracellular single-stranded DNA (ssDNA) is accumulated during pathogen infection or pathogenesis, it remains unclear whether and how intracellular ssDNA stimulates the immune system. We report that intracellular ssDNA triggers cytokine expression and cell death in a CGT motif-dependent manner. Through a genome-wide CRISPRLCas9 screen, we identified Schlafen-11 (SLFN11), which is essential for the response to endogenous and pathogen ssDNA. SLFN11 directly binds ssDNA containing CGT motifs and translocates to the cytoplasm upon ssDNA recognition. The mice deficient in SLFN9, the homologue of SLFN11, were resistant to CGT ssDNA-induced inflammation, acute hepatitis and septic shock. This study establishes CGT ssDNA and SLFN11/9 as a novel type of immunostimulatory nucleic acids and pattern recognition receptors, respectively.One-sentence summariessingle-stranded DNA inside cells can awaken Schlafens with a CGT motif to trigger cytokine expression and lytic cell death.
Clinical scenario related to cardiovascular system: is it possible to develop thoracic pain imitating a musculoskeletal disorder? A scoping review
Authors: De Meo Nicola , Poselek David , Margelli Michele , Segat Andrea , Zaninetti Martina , Segat Marco , Minetti Federico , Galeoto Giovanni , Maselli Filippo , Fascia Matteo 
Published: February 2024

AbstractBackgroundWhen assessing a patient presenting with thoracic pain it’s important for the physiotherapist to quickly understand if the cause is a musculoskeletal condition or a dysfunction of the cardiovascular and circulatory system. Promptly referring the patient is essential to identify potentially life-threatening conditions at an early stage.ObjectivesIdentifying the current state of knowledge regarding cardiovascular and circulatory systems conditions that generate a thoracic pain that resembles a musculoskeletal condition.Study DesignScoping reviewEligibility criteriaThis review will incorporate studies encompassing any research design. Inclusion is limited to articles written in either English or Italian language. Our population of interest specifically includes patients experiencing thoracic pain, with no restrictions regarding age and gender, to ensure a comprehensive understanding of the condition’s impact across different demographics. The concept under investigation is the manifestation of symptoms in the thoracic region, which are attributed to cardiovascular disorders or dysfunctions. It is critical to our scope that we delineate the context by intentionally omitting studies set in emergency contexts. This exclusion criterion allows the review to narrow its focus on the tools employed in making differential diagnoses without relying on instrumental examinations, thus aiming to elucidate diagnostic strategies applicable in a non-emergent setting.ResultsConclusions
Stimulation of saliva affects the release of aroma in wine; a study of microbiota, biochemistry and participant origin
Authors: Ruan Xinwei , Chen Yipeng , Chauhan Aafreen , Howell Kate S. 
Published: February 2024

AbstractSaliva influences the release of aroma in the oral cavity. The composition of human saliva varies depending on stimulation and host’s origin; however, the compositional differences of saliva and their influences on aroma release have not been fully evaluated. In this study, we recruited 30 healthy adults (15 Australians and 15 Chinese) and collected saliva samples at three stages: before, during, and after stimulation. Salivary samples were characterised by flow rate, total protein concentration, esterase activity, microbiome composition by full-length 16S rRNA gene sequencing, and the ability to release aroma from wine by headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS). Differences in salivary composition and specific wine volatiles were found between Australian and Chinese participants, and amongst the three stimulation stages. Significant correlations between the relative abundance of 3 bacterial species and 10 wine volatiles were observed. Our results confirm the influence of participant’s geographic origin and stimulation on salivary composition, highlighting the role of salivary components, especially salivary bacteria, on the release of aroma from wine.
Bioactive coatings on 3D printed scaffolds for bone regeneration: Use of Laponite®to deliver BMP-2 in an ovine femoral condyle defect model
Authors: Marshall Karen M. , McLaren Jane S. , Wojciechowski Jonathan P. , Callens Sebastien J. P. , Echalier Cécile , Kanczler Janos M. , Rose Felicity R. A. J. , Stevens Molly M. , Dawson Jonathan I. , Oreffo Richard O.C. 
Published: February 2024

AbstractBiomaterial-based approaches for bone regeneration seek to explore alternative strategies to repair non-healing fractures and critical-sized bone defects. Fracture non-union occurs due to a number of factors resulting in the formation of bone defects. Rigorous evaluation of the biomaterials in relevant models and assessment of their potential to translate towards clinical use is vital. Large animal experimentation can be used to model fracture non-union while scaling-up materials for clinical use. Growth factors modulate cell phenotype, behaviour and initiate signalling pathways leading to changes in matrix deposition and tissue formation. Bone morphogenetic protein-2 (BMP-2) is a potent osteogenic growth factor, with a rapid clearance timein vivonecessitating clinical use at a high dose, with potential deleterious side-effects. The current studies have examined the potential for Laponite®nanoclay coated poly(caprolactone) trimethacrylate (PCL-TMA900) scaffolds to bind BMP-2 for enhanced osteoinduction in a large animal critical-sized bone defect. An ovine femoral condyle defect model confirmed PCL-TMA900 scaffolds coated with Laponite®/BMP-2 produced significant bone formation compared to the uncoated PCL-TMA 900 scaffoldin vivo, assessed by micro-computed tomography (µCT) and histology. This indicated the ability of Laponite®to deliver the bioactive BMP-2 on the PCL-TMA900 scaffold. Bone formed around the Laponite®/BMP-2 coated PCL-TMA900 scaffold, with no erroneous bone formation observed away from the scaffold material confirming localisation of BMP-2 delivery. The current studies demonstrate the ability of a nanoclay to localise and deliver bioactive BMP-2 within a tailored octet-truss scaffold for efficacious bone defect repair in a large animal model with significant implications for translation to the clinic.
Single cell sequencing reveals cellular landscape alterations in the airway mucosa of patients with pulmonary long COVID
Authors: Gerayeli Firoozeh V. , Park Hye Yun , Milne Stephen , Li Xuan , Yang Chen Xi , Tuong Josie , Eddy Rachel L , Guinto Elizabeth , Cheung Chung Y , Yang Julia SW , Gilchrist Cassie , Abbas Dina , Stach Tara , Leung Clarus , Shaipanich Tawimas , Leipsic Jonathan , Koelwyn Graeme , Leung Janice M. , Sin Don D. 
Published: February 2024

AbstractTo elucidate the important cellular and molecular drivers of pulmonary long COVID, we generated a single-cell transcriptomic map of the airway mucosa using bronchial brushings from patients with long COVID who reported persistent pulmonary symptoms.Adults with and without long COVID were recruited from the general community in greater Vancouver, Canada. The cohort was divided into those with pulmonary long COVID (PLC), which was defined as persons with new or worsening respiratory symptoms following at least one year from their initial acute SARS-CoV-2 infection (N=9); and control subjects defined as SARS-CoV-2 infected persons whose acute respiratory symptoms had fully resolved or individuals who had not experienced acute COVID-19 (N=9). These participants underwent bronchoscopy from which a single cell suspension was created from bronchial brush samples and then sequenced.A total of 56,906 cells were recovered for the downstream analysis, with 34,840 cells belonging to the PLC group. A dimensionality reduction plot shows a unique cluster of neutrophils in the PLC group (p<.05). Ingenuity Pathway Analysis revealed that neutrophil degranulation pathway was enriched across epithelial cells. Differential gene expression analysis between the PLC and control groups demonstrated upregulation of mucin genes in secretory cell clusters.A single-cell transcriptomic landscape of the small airways shows that the PLC airways harbors a dominant neutrophil cluster and an upregulation in the neutrophil-associated activation signature with increased expression of MUC genes in the secretory cells. Together, they suggest that pulmonary symptoms of long COVID may be driven by chronic small airway inflammation.Take home messageSingle cell profiling shows the infiltration of neutrophils with upregulation of mucin genes in the airway mucosa of patients with pulmonary long COVID, indicating persistent small airway inflammation in pulmonary long COVID.