bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arthritis and the family
Authors: Reisine Susan T. 
Published: February 2007

AbstractObjectives. This paper reviews the literature on arthritis and the family in two areas, juvenile rheumatic diseases and adult‐onset arthritis.Methods. All published papers related to arthritis and the family were identified through a Medline search and through hand searching of the major rheumatology journals.Results. The literature on the family and juvenile arthritis is somewhat inconsistent, in that some studies demonstrate significant psychosocial impact among children and families, while others find no differences in children who have arthritis compared to normative data or to siblings. Lack of consistency in the literature is largely related to methodologic problems, as most studies are retrospective reports, consisting of relatively small, nonrepresentative samples of children with rheumatic diseases. The influence of adult‐onset rheumatoid arthritis and osteoarthritis on family role functioning and the performance of household responsibilities is well documented, and a body of literature on the importance of family functioning to well‐being is accumulating.Conclusions. Future studies should expand sample sizes in order to investigate family impact on juvenile rheumatoid arthritis more thoroughly. More studies on the factors that contribute to improved family functioning and subsequent improvements in well‐being, especially in conditions other than rheumatoid arthritis and osteoarthritis, are needed. Intervention studies to reduce perceived pain and functional disability in arthritis by incorporating a family‐systems perspective are particularly lacking.
The impact of rheumatoid arthritis on life activities
Authors: Katz Patricia P. 
Published: February 2007

AbstractObjective. To examine the proportion of valued activities performed by persons with rheumatoid arthritis (RA) and the change in this proportion over a 5‐year period and to compare the performance and loss of valued activities of persons with and without RA.Methods. Data were drawn from a longitudinal panel study of persons with RA. Valued activities were assessed using a standardized questionnaire.Results. Over a 5‐year period, persons with RA lost approximately 10% of the activities they had valued. Losses were noted in every domain of activity. The greatest losses were seen in work‐related, service, nurturant, cultural and leisure, and social participation activities. Compared to persons without arthritis, persons with RA performed fewer valued activities at baseline, and lost more valued activities over the 5‐year period.Conclusions. RA takes a considerable toll on the ability of people with the disease to perform valued life activities. Other research has shown that the loss of valued activities is a strong risk factor for the development of depressive symptoms among women with RA.
The psychological impact of arthritis: Prevalence of depression
Authors: DeVellis Brenda McEvoy 
Published: February 2007

AbstractAttempts to estimate the prevalence of depression in people with arthritis are complicated by conceptual, definitional, and measurement problems and inconsistencies. Following a description of these methodologic problems, recent studies of depressive disorders and depressive symptoms in clinical and community samples are reviewed. The review suggests that although most people with rheumatic diseases do not experience them, depressive disorders and symptoms are probably more prevalent among people with rheumatic diseases than among people with no chronic diseases. This somewhat increased prevalence is probably no greater than that found in other serious chronic diseases, however. Additional research is needed.
From early twinges to mastery: The process of adjustment in living with rheumatoid arthritis
Authors: Shaul Muriel P. 
Published: February 2007

AbstractObjective.To describe the experiences of women who have learned to live with rheumatoid arthritis (RA), and to explore how women manage this chronic illness and the demands of their everyday lives.Methods.Thirty women with a diagnosis of RA were interviewed in their homes. The women were encouraged to “tell their story of living with RA,” from the first symptom to the present, using a semistructured questionnaire. Qualitative data were analyzed using a constant comparative analytic process.Results.Learning to live with RA was characterized by periods of uncertainty, of learning about the illness, discovering strategies that allowed the women to meet the expectations of their roles as women, and the establishment of new patterns of daily life. The women became experts at managing their RA and developed a sense of mastery in coping with their symptoms.Conclusions.Persons with RA need support and guidance, beginning with the onset of the disease, to facilitate achieving mastery in living with this chronic illness.
Musculoskeletal conditions and employment
Authors: Yelin Edward H. 
Published: February 2007

AbstractObjective. To describe the prevalence, incidence, and correlates of work disability among persons with musculoskeletal conditions.Methods. Literature review and analysis of the Health and Retirement Survey, a national, probability sample of community‐based adults ages 51–61.Results. Clinical samples of persons with RA report work disability rates of 51% to 60%. Communitybased studies of persons with a broad range of musculoskeletal conditions find work disability rates of 38% to 72%, depending on the mix of symptoms and particular sampling strategy. Persons with musculoskeletal conditions and comorbidity are particularly prone to work loss, with three‐quarters leaving work prior to the normal age of retirement. In multivariate models, after adjusting for demographic and functional characteristics, persons with musculoskeletal conditions actually are more likely to work than those without.Conclusions. Persons with musculoskeletal conditions have high rates of work disability, but given their level of impairment, are more likely to work than persons without musculoskeletal conditions.
Synthesis of [18,19,21,22‐13C4]‐Labeled Tautomycin as an NMR Probe of Protein Phosphatase Inhibitor
Authors: Isobe Minoru , Kurono Masakuni , Tsuboi Katsunori , Takai Akira 
Published: February 2007

AbstractWe have accomplished the synthesis of 13C‐labeled tautomycin at the C18, C19, C21, and C22 positions starting from 100 % [13C]triethylphosphonoacetate for the purpose of elucidating the dynamics and conformation of the C17–C26 moiety. NMR spectroscopy of 13C‐labeled tautomycin revealed strong binding with protein phosphatase type 1 and new features in the 13C NMR spectrum, such as the very small three‐bond coupling constants (2J).
Accelerated formation of multicellular 3‐D structures by cell‐to‐cell cross‐linking
Authors: De Bank Paul A. , Hou Qingpu , Warner Robert M. , Wood Ian V. , Ali Bahaa E. , MacNeil Sheila , Kendall David A. , Kellam Barrie , Shakesheff Kevin M. , Buttery Lee D.K. 
Published: February 2007

AbstractThe three‐dimensional (3‐D) arrangement of cells within tissues is integral to their development and function. Advances in stem cell science and regenerative medicine have stimulated interest in the replication of this architecture in vitro. We have developed a versatile method for controlling short‐term cell–cell and cell–matrix interactions via a facile cell surface engineering process that enables the rapid formation of specific 3‐D interactions for a range of cell types. We demonstrate that chemical modification of cell surfaces and matrix proteins can artificially accelerate the cell adhesion process and confirm the ability to control the formation of multicellular aggregates with defined architectures and heterotypic cell types. Direct comparison with a natural aggregation process seen during differentiation of embryonic stem (ES) cells revealed increased expression of developmental regulatory proteins and a concomitant enhancement of ES cell differentiation. Furthermore, this new methodology has numerous applications in generating layered structures. For example, we demonstrate improved transfer of therapeutic human keratinocytes onto a dermal layer in a skin repair model. Biotechnol. Bioeng. 2007; 97: 1617–1625. © 2007 Wiley Periodicals, Inc.
Bacterial oxidation of ferrous iron at low temperatures
Authors: Kupka Daniel , Rzhepishevska Olena I. , Dopson Mark , Lindström E. Börje , Karnachuk Olia V. , Tuovinen Olli H. 
Published: February 2007

AbstractThis study comprises the first report of ferrous iron oxidation by psychrotolerant, acidophilic iron‐oxidizing bacteria capable of growing at 5°C. Samples of mine drainage‐impacted surface soils and sediments from the Norilsk mining region (Taimyr, Siberia) and Kristineberg (Skellefte district, Sweden) were inoculated into acidic ferrous sulfate media and incubated at 5°C. Iron oxidation was preceded by an approximately 3‐month lag period that was reduced in subsequent cultures. Three enrichment cultures were chosen for further work and one culture designated as isolate SS3 was purified by colony isolation from a Norilsk enrichment culture for determining the kinetics of iron oxidation. The 16S rRNA based phylogeny of SS3 and two other psychrotolerant cultures, SS5 from Norilsk and SK5 from Northern Sweden, was determined. Comparative analysis of amplified 16S rRNA gene sequences showed that the psychrotolerant cultures aligned within Acidithiobacillus ferrooxidans. The rate constant of iron oxidation by growing cultures of SS3 was in the range of 0.0162–0.0104 h−1 depending on the initial pH. The oxidation kinetics followed an exponential pattern, consistent with a first order rate expression. Parallel iron oxidation by a mesophilic reference culture of Acidithiobacillus ferrooxidans was extremely slow and linear. Precipitates harvested from the 5°C culture were identified by X‐ray diffraction as mixtures of schwertmannite (ideal formula Fe8O8(OH)6SO4) and jarosite (KFe3(SO4)2(OH)6). Jarosite was much more dominant in precipitates produced at 30°C. Biotechnol. Bioeng. 2007;97:1470–1478. © 2007 Wiley Periodicals, Inc.
Concurrent nitrite oxidation and aerobic denitrification in activated sludge exposed to volatile fatty acids
Authors: Oguz Merve T. , Robinson Kevin G. , Layton Alice C. , Sayler Gary S. 
Published: February 2007

AbstractThe goal of this research was to investigate the simultaneous occurrence of nitrification and denitrification by activated sludge exposed to volatile fatty acids (VFAs) during aerobic wastewater treatment using a single‐stage reactor. A mixture of VFAs was spiked directly into a continuous‐stirred tank reactor (CSTR) to assess subsequent impacts on nitrite removal, nitrate formation, CO2 fixation, total bacterial density, and dominant nitrite oxidizing bacteria (NOB) concentration (i.e., Nitrospira). The activity of the periplasmic nitrate reductase (NAP) enzyme and the presence of nap gene were also measured. A rapid decrease in the nitrate formation rate (>70% reduction) was measured for activated sludge exposed to VFAs; however, the nitrite removal rate was not reduced. The total bacterial density and Nitrospira concentration remained essentially constant; therefore, the reduction in nitrate formation rate was likely not due to heterotrophic uptake of nitrogen or to a decrease in the dominant NOB population. Additionally, VFA exposure did not impact microbial CO2 fixation efficiency. The activity of NAP enzyme increased in the presence of VFAs suggesting that nitrate produced as a consequence of nitrite oxidation was likely further reduced to gaseous denitrification products via catalysis by NAP. Little, if any, nitrogen was discharged in the aqueous effluent of the CSTR after exposure to VFAs demonstrating that activated sludge treatment yielded compounds other than those typically produced solely by nitrification. Biotechnol. Bioeng. 2007; 97: 1562–1572. © 2007 Wiley Periodicals, Inc.
Cryopreservation in micro‐volumes: Impact upon caco‐2 colon adenocarcinoma cell proliferation and differentiation
Authors: Malpique Rita , Katsen‐Globa Alisa , Carrondo Manuel J.T. , Zimmermann Heiko , Alves Paula M. 
Published: February 2007

AbstractRecent advances in cell‐based therapies require new approaches for cell cryopreservation, capable of dealing with large number of samples and providing specific conditions for each cell type. Reduction of sample volume from the commonly used 1 mL to 25 µL in 30‐well micro‐cryosubstrates improves cryopreservation by allowing automation, data handling and access to individual wells without thawing the whole cryosubstrate. This system was evaluated for the storage of Caco‐2 colon adenocarcinoma cells, which differentiate spontaneously after long‐term culture. The impact of the cryosample small volume upon post‐thawing membrane integrity of the cells and their capacity to proliferate and differentiate was studied. Two different cryoprotectants commonly employed, dimethyl sulfoxide (Me2SO) and glycerol, were evaluated as well as the possibility of decreasing their concentration from the 10% concentration, usually used, down to 3% (v/v). The process automation by pipette robotic addition of the cryoprotectant to the micro‐cryosubstrates was also evaluated. The micro‐cryosubstrates have proven to be at least as efficient as typical 1 mL cryovials for cryopreservation of Caco‐2 cells using either Me2SO or glycerol. Compared to the manual process, the automatic addition of glycerol to the micro‐cryosubstrates allowed higher cell viabilities after thawing while with Me2SO no significant changes were observed. Me2SO has shown to be more effective than glycerol in maintaining high post‐thaw cell membrane integrity, either in micro‐cryosubstrates or cryovials, for any of the concentrations tested. The ability of Me2SO in maintaining high cell membrane integrity post‐thawing was confirmed by long‐term (up to 22 days) proliferation and differentiation studies performed with cells cultured immediately after thawing. Biotechnol. Bioeng. 2007; 98: 155–166. © 2007 Wiley Periodicals, Inc.
Functional Studies of Synthetic Gramicidin Hybrid Ion Channels in CHO Cells
Authors: Wesolowski Ryszard , Sommer Anna , Arndt Hans‐Dieter , Koert Ulrich , Reiß Philipp , Wimmers Sönke , Strauss Olaf 
Published: February 2007

AbstractThe function of a gramicidin hybrid ion channel in living Chinese hamster ovary (CHO) cells was investigated by the patch clamp method. The synthetic ion channel 1 consists of two cyclohexyl ether amino acids that link two minigramicidin strands. With 1 at a concentration of 1.0 μM, an increase in the whole‐cell membrane conductance was observed after 1.37 min. The conductance showed larger currents when Cs+ was used as charge carrier than when Na+ and K+ were used. In single‐channel recordings with Cs+ as charge carrier, the substance showed comparable single‐channel amplitudes in the membrane of living cells and artificial black lipid bilayers. In addition to functioning as a cation channel, compound 1 appeared to be a water channel. Exposure of the CHO cells to an extracellular hypoosmotic solution did not substantially change the cell volume. Extracellular hypoosmotic conditions in the presence of 1 increased the cell size to 146.5 % that of the control. Thus, the synthetic hybrid channel 1 can function as a cation channel with some Cs+ specificity, and as a water channel in CHO cells.
A Self‐Immolative Reporter For β‐Galactosidase Sensing
Authors: Ho Nan‐Hui , Weissleder Ralph , Tung Ching‐Hsuan 
Published: February 2007

AbstractA dual fluorogenic and chromogenic probe, Gal–2SBPO, has been developed based on a synthesized water‐soluble dye, 9‐di‐3‐sulfonyl‐propylaminobenzo[a]phenoxazonium perchloride (2SPBO, λex/em=630/670 nm). β‐D‐Galactopyranoside, the substrate of β‐galactosidase, was conjugated to 2SBPO through a para‐substituted benzyloxycarbonyl group and a glycine residue, which serve as a self‐immolative spacer and as a molecular blocker to mask the optical signal of 2SBPO, respectively. Gal–2SBPO was soluble and stable under physiological conditions. Enzymatic cleavage of the β‐D‐galactopyranoside triggered a series of spontaneous reactions that resulted in a release of optically active 2SBPO. The β‐galactosidase activity was assayed by monitoring the absorbance at 630 nm and fluorescence at 670 nm.
Porous Iron Oxide Based Nanorods Developed as Delivery Nanocapsules
Authors: Wu Ping‐Ching , Wang Wen‐Shiuan , Huang Ying‐Ting , Sheu Hwo‐Shuenn , Lo Yi‐Wei , Tsai Tsung‐Lin , Shieh Dar‐Bin , Yeh Chen‐Sheng 
Published: February 2007

AbstractA low‐temperature solution approach (90–95 °C) using FeCl3 and urea was carried out to synthesize β‐FeOOH nanorods in aqueous solution. The as‐synthesized β‐FeOOH nanorods were further calcined at 300 °C to form porous nanorods with compositions including both β‐FeOOH and α‐Fe2O3. The derived porous nanorods were engineered to assemble with four layers of polyelectrolytes (polyacrylic acid (PAA)/polyethylenimine(PEI)/PAA/PEI) on their surfaces as polyelectrolyte multilayer nanocapsules. Fluorescein isothiocyanate (FITC) molecules were loaded into the polyelectrolyte multilayer nanocapsules in order to investigate drug release and intracellular delivery in Hela cells. The as‐prepared nanocapsules showed ionic strength‐dependent control of the permeability of the polyelectrolyte shells. The release behavior of the entrapped FITC from the FITC‐loaded nanocapsules exhibited either controlled‐ or sustained‐release trends, depending on the compactness of the polyelectrolyte shells on the nanorod surfaces. Cytotoxicity measurements demonstrate that the native nanorods and the polymer‐coated nanorods have excellent biocompatibility in all dosages between 0.1 ng mL−1 and 100 μgm L−1. The time dependence of uptake of FITC‐loaded nanocapsules by Hela cancer cells observed by laser confocal microscopy indicates that the nanocapsules can readily be taken up by cancer cells in 15 min, a relatively short period of time, while the slow release of the FITC from the initial perimembrane space into the cytoplasm was followed by release into the nucleus after 24 h.
Efficient H/D Exchange Reactions of Alkyl‐Substituted Benzene Derivatives by Means of the Pd/C–H2–D2O System
Authors: Esaki Hiroyoshi , Aoki Fumiyo , Umemura Miho , Kato Masatsugu , Maegawa Tomohiro , Monguchi Yasunari , Sajiki Hironao 
Published: February 2007

AbstractA method for efficient and extensive H/D exchange of substituted benzene derivatives which is catalyzed by heterogeneous Pd/C in D2O as a deuterium source under hydrogen atmosphere is described. Multideuterium incorporation into unactivated linear or branched alkyl chains that bear a carboxyl, hydroxyl, ether, ester, or amide moiety and are connected with a benzene ring was achieved by using the Pd/C–H2–D2O system. The present method does not require expensive deuterium gas or any special equipment.
Cardiac angiosarcoma: Report of a case diagnosed by echocardiographic‐guided fine‐needle aspiration
Authors: Bhalla Ritu , Nassar Aziza 
Published: February 2007

AbstractThe authors present a case of cardiac angiosarcoma diagnosed by fine‐needle aspiration (FNA) in a 33‐year‐old male. A percutaneous right atrial mass biopsy was performed via a right internal jugular venous approach and under echocardiographic guidance. FNA showed pleomorphic spindle‐shaped cells present singly and in small clusters in a background of hemorrhage. Occasional pseudo‐acini or microacinar structures were observed, that are lined with abnormal spindly to polygonal cells, indicative of vasoformative structures. Immunostain for CD31 was strongly positive. A diagnosis of cardiac angiosarcoma was made, following which, the patient underwent treatment with chemotherapy. Diagn. Cytopathol. 2007;35:164–166. © 2007 Wiley‐Liss, Inc.
Clear cell and eosinophilic oncocytomas of salivary gland: Cytological variants or parallels?
Authors: Saqi Anjali , Giorgadze Tamar A. , Eleazar Jennifer , Remotti Fabrizio , Vazquez Madeline F. 
Published: February 2007

AbstractOncocytomas are uncommon tumors of the salivary gland. They have an abundance of mitochondria, which is manifested as granular eosinophilic cytoplasm by light microscopy. On histological sections, presence of cytoplasmic glycogen and/or fixation artifact can impart cytoplasmic clearing, and oncocytomas with a predominance of clear cytoplasm are labeled clear cell oncocytomas. Two forms of oncocytoma, eosinophilic and clear cell, have been described in the surgical pathology literature. The purpose of this manuscript is to conduct a comparative cytological assessment to ascertain parallels and differences between the two variants. Diagn. Cytopathol. 2007;35:158–163. © 2007 Wiley‐Liss, Inc.
Immunocytochemical study of the expression of mesothelin in fine‐needle aspiration biopsy specimens of pancreatic adenocarcinoma
Authors: Baruch Amy C. , Wang Huamin , Staerkel Gregg A. , Evans Douglas B. , Hwang Rosa Frances , Krishnamurthy Savitri 
Published: February 2007

AbstractMesothelin is a potential marker of pancreatic adenocarcinoma that was recently identified by serial analysis of gene expression. We evaluated the sensitivity and specificity of mesothelin as a marker of pancreatic adenocarcinoma on destained Papanicolaou (Pap) smears and unstained cellblocks from 28 patients using a monoclonal antibody to mesothelin. Intensity and proportion of staining was semiquantitatively graded on a scale of 1–3, and as 0%, 1 to <10%, 10–50%, or >50%. Positive staining for mesothelin was seen in 64% of the direct smears and in 36% of cell block sections. Focal positivity for mesothelin was noted in benign pancreatic tissue in one of 10 cases. Staining was most often focal (<50% of cells) in both direct smears and cell block sections. The overall sensitivity and specificity of mesothelin as a marker for pancreatic adenocarcinoma were 68% and 90%, respectively. Sensitivity was higher in Pap smears than in cell block sections (64% versus 36%). The presence of occasional mesothelin expression in benign tissue, its very focal expression in malignant tissue may limit the utility of mesothelin as a marker of pancreatic adenocarcinomas in fine‐needle aspiration (FNA) specimens. Diagn. Cytopathol. 2007;35:143–147. © 2007 Wiley‐Liss, Inc.
Karyomegalic interstitial nephritis: A pitfall in urine cytology
Authors: Palmer David , Lallu Sharda , Matheson Philip , Bethwaite Peter , Tompson Keneth 
Published: February 2007

AbstractA case of a 44‐yr‐old Maori female presenting with chronic renal failure due to karyomegalic interstitial nephritis is reported. To our knowledge, this is the first instance this disorder has been reported in a New Zealand Maori.Karyomegalic interstitial nephritis is a rare cause of progressive renal failure, which may be familial, and is frequently associated with a history of recurrent respiratory infections.The urine in this case contained large, pleomorphic cells, mimicking carcinoma. The case therefore illustrates a rare cause of chronic renal failure, and presents an important diagnostic pitfall in the practice of urine cytopathology. Diagn. Cytopathol. 2007;35:179–182. © 2007 Wiley‐Liss, Inc.
Fine‐needle aspiration of malignant mesothelioma with unusual morphologic features: A case report
Authors: Kapur Umesh , Wojcik Eva M. 
Published: February 2007

AbstractMalignant mesothelioma is an aggressive neoplasm linked to asbestos exposure. Most mesothelioma patients present with pleural effusion and the fluid is typically sent for cytological examination. Therefore, cytopathologists are most familiar with features of mesothelioma in fluid preparations. We present here a case of malignant mesothelioma with unusual cytological features diagnosed on FNA. The diagnosis was confirmed by immunohistochemcal and electron microscopic studies. In addition, we compare the cytomorphological features observed in malignant effusion versus fine‐needle aspiration. Diagn. Cytopathol. 2007;35:174–178. © 2007 Wiley‐Liss, Inc.