bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple risk factors related to familial predisposition to anterior cruciate ligament injury: fraternal twin sisters with anterior cruciate ligament ruptures
Authors: Hewett T E , Lynch T R , Myer G D , Ford K R , Gwin R C , Heidt R S 
Published: January 2009

ObjectiveA multifactorial combination of predictors may increase anterior cruciate ligament (ACL) injury risk in athletes. The objective of this twin study was to examine these risk factors to identify commonalities in risk factors that predisposed female fraternal twins to ACL injury.MethodsFemale twins in high-risk sports were prospectively measured prior to an injury for neuromuscular control using three-dimensional motion analysis during landing, hamstrings and quadriceps muscular strength on a dynamometer and joint laxity using a modified Beighton–Horan index and a Compu-KT arthrometer. Intraoperative measures of femoral intercondylar notch width were recorded during ACL reconstruction.ResultsAbduction angles were increased at one knee in both of the twin sister athletes relative to uninjured controls at initial contact and at maximum displacement during landing. The twin female athletes that went on to ACL injury also demonstrated decreased peak knee flexion motion at both knees than uninjured females during landing. The twin athletes also had increased joint laxity and decreased hamstrings to quadriceps (H/Q) torque ratios compared to controls. Femoral intercondylar notch widths were also below the control mean in the twin siblings.ConclusionsPrescreened mature female twins that subsequently experienced ACL injury demonstrated multiple potential risk factors including: increased knee abduction angles, decreased knee flexion angles, increased general joint laxity, decreased H/Q ratios and femoral intercondylar notch width.
Cancer risks in chemical production workers exposed to 2-mercaptobenzothiazole
Authors: Sorahan T 
Published: January 2009

Objectives:To investigate cancer risks in chemical production workers exposed to 2-mercaptobenzothiazole (MBT).Methods:The mortality (1955–2005) and cancer morbidity experience (1971–2005) of a cohort of 363 male production workers exposed to MBT while employed at a chemical factory in north Wales were investigated. Two analytical approaches were used, indirect standardisation and Poisson regression.Results:Based on national mortality rates, significant excess mortality was found for cancers of the large intestine (observed; Obs 8, standardised mortality ratio (SMR) 232, 95% CI 100 to 457) and bladder (Obs 8, SMR 374, 95% CI 162 to 737). Non-significant excesses were shown for lung cancer (Obs 27, SMR 138, 95% CI 91 to 201) and multiple myeloma (Obs 3, SMR 440, 95% CI 91 to 1287). Based on national cancer incidence rates, significant excess morbidity was found for cancer of the bladder (Obs 12, standardised registration ratio (SRR) 253, 95% CI 131 to 441) and multiple myeloma (Obs 4, SRR 465, 95% CI 127 to 1191). Non-significant excesses were shown for cancers of the large intestine (Obs 9, SRR 181, 95% CI 83 to 344) and lung (Obs 26, SRR 152, 95% CI 99 to 223). In analyses that included follow-up information on an additional 1797 plant employees not exposed to MBT, Poisson regression showed significant positive trends both for risks of cancer of the large intestine and for risks of multiple myeloma in relation to estimated cumulative exposure to MBT.Conclusions:MBT may be a human carcinogen; confident evaluation awaits findings from other studies.
Clinical evaluation of a non-immersive virtual environment in stroke rehabilitation
Authors: Edmans Judi , Gladman John , Hilton Dave , Walker Marion , Sunderland Alan , Cobb Sue , Pridmore Tony , Thomas Shirley 
Published: January 2009

Background and purpose: We describe our attempts to evaluate the effectiveness of a virtual environment developed to rehabilitate stroke patients in the task of making a hot drink. Methods: Single case studies were performed in 13/138 (9%) stroke patients undergoing rehabilitation in a UK stroke unit. Participants in AB/BA (n = 5) and ABA (n = 2) design studies received 5 one-hour sessions of attention control training (A phase) and 5 one-hour sessions of virtual environment training (B phase). An AB design with random duration of A and B phases (minimum duration of A and B phases 3 and 5 days respectively, with total duration of 3 weeks) was used in 6 participants. Results: Visual inspection of scores across all cases showed a trend towards improvement over time in both real and virtual hot drink making ability in both control and intervention phases. There was no significant difference (Wilcoxon, p > 0.05) in the improvements in real and virtual hot drink making ability during all control and intervention phases in the 13 cases. Ceiling effects limited the evaluation of effectiveness in 5 of the 8 cases in which daily performance measures were used. Conclusions: Few people in this setting were suitable for this intervention. The case studies showed no evidence of a strong effect of this intervention, but we had great difficulty in performing single case studies. We conclude that more testing and development of this system is required before it is subjected to rigorous testing of clinical effectiveness.
Efficacy of pneumatic compression and low-level laser therapy in the treatment of postmastectomy lymphoedema: a randomized controlled trial
Authors: Kozanoglu Erkan , Basaran Sibel , Paydas Semra , Sarpel Tunay 
Published: January 2009

Objective: To compare the long-term efficacy of pneumatic compression and low-level laser therapies in the management of postmastectomy lymphoedema. Design: Randomized controlled trial. Setting: Department of Physical Medicine and Rehabilitation of Cukurova University, Turkey. Subjects: Forty-seven patients with postmastectomy lymphoedema were enrolled in the study. Interventions: Patients were randomly allocated to pneumatic compression (group I, n=24) and low-level laser (group II, n=23) groups. Group I received 2 hours of compression therapy and group II received 20 minutes of laser therapy for four weeks. All patients were advised to perform daily limb exercises. Main measures: Demographic features, difference between sum of the circumferences of affected and unaffected limbs (▵C), pain with visual analogue scale and grip strength were recorded. Results: Mean age of the patients was 48.3 (10.4) years. ▵C decreased significantly at one, three and six months within both groups, and the decrease was still significant at month 12 only in group II (P = 0.004). Improvement of group II was greater than that of group I post treatment (P = 0.04) and at month 12 after 12 months (P = 0.02). Pain was significantly reduced in group I only at posttreatment evaluation, whereas in group II it was significant post treatment and at follow-up visits. No significant difference was detected in pain scores between the two groups. Grip strength was improved in both groups, but the differences between groups were not significant. Conclusions: Patients in both groups improved after the interventions. Group II had better long-term results than group I. Low-level laser might be a useful modality in the treatment of postmastectomy lymphoedema.
Mental techniques during manual stretching in spasticity — a pilot randomized controlled trial
Authors: Bovend'Eerdt Thamar JH , Dawes Helen , Sackley Cath , Izadi Hooshang , Wade Derick T 
Published: January 2009

Objective: To evaluate the feasibility and effects of using motor imagery during therapeutic stretching in individuals with spasticity. Design: Randomized single-blind controlled pilot trial. Setting: Chronic day care unit, neurological rehabilitation unit and in the community. Subjects: Eleven individuals with spasticity in the arm requiring stretching as part of their normal routine. Interventions: In addition to their normal stretching routine, subjects in the experimental group received motor imagery during their stretches (n = 6). The control group received progressive muscle relaxation during their stretches (n = 5). The dose varied between 8 and 56 sessions over eight weeks. Main measures: Resistance to passive movement, measured with a torque transducer, passive range of movement, measured with an electro-goniometer, Modified Ashworth Scale (MAS) and level of discomfort during the MAS were assessed at baseline and after eight weeks by an independent assessor. These measures were recorded before and after a stretch intervention on both assessments. Results: Participants, therapists and carers tolerated the techniques well. Compliance was variable and adherence was good. Mixed ANOVA showed no difference over time and no difference between the motor imagery and progressive muscle relaxation group on the primary and secondary outcome measures (P>0.05). Conclusions: It is feasible to use motor imagery during therapeutic stretching. Statistical power was low due to the large variability in the population and the small sample size. Post-hoc sample size calculation suggests that future studies of this subject should include at least 54 participants per group. Further research is warranted.
An exploratory cluster randomized controlled trial of group exercise on mobility and depression in care home residents
Authors: Brittle Nicola , Patel Smitaa , Wright Christine , Baral Sabrina , Versfeld Pam , Sackley Catherine 
Published: January 2009

Objective: To investigate the feasibility, acceptability and potential efficacy of group exercise for residents in care homes. Design: Exploratory cluster randomized controlled trial. Setting: Five randomly selected care homes in South Birmingham, UK. Participants: Fifty-six care home residents (mean age 84.5, 71% female), 39 (70%) with cognitive impairments. Intervention: Two homes (n = 28) were randomized to group exercise held twice weekly for five weeks. The remaining three homes (n = 28) formed the control group and received usual care, with no person specifically responsible for exercise training. Outcome measures: Assessments were conducted at zero (pre-intervention), three (post-intervention) and six months (follow-up) using the Rivermead Mobility Index and Hospital Anxiety and Depression Scale or Stroke Aphasic Depression Questionnaire (depending on cognitive impairment). Adherence to group exercise and retention to the study were also documented. Results: No statistically significant improvements in mobility or depression were found in favour of group exercise. Retention to the study was high with 46 (82%) participants completing all assessments. Adherence to group exercise was somewhat lower with participants attending a mean of 3.61 out of 8.5 prescribed sessions (42.5%). Conclusions: Group exercise can be delivered to care home residents with reduced mobility but it is not suitable for residents with severe cognitive impairment. An estimated sample size of 240 participants would be required to detect a clinically significant difference in the Rivermead Mobility Index with 90% power.
Short-term effects of aerobic exercise on functional capacity, fatigue, and quality of life in patients with post-polio syndrome
Authors: Oncu Jülide , Durmaz Berrin , Karapolat Hale 
Published: January 2009

Objective: To investigate and compare the impact of hospital and home exercise programmes on aerobic capacity, fatigue, and quality of life in patients with post-polio syndrome. Design: A prospective, randomized controlled trial. Setting: Department of Physical Medicine and Rehabilitation, University Hospital. Subjects: Thirty-two patients were divided into two groups for either hospital- or home-based aerobic exercise programme. Main outcome measures: Patients were assessed before and after the rehabilitation programme, with respect to functional capacity (pVo2), fatigue (Fatigue Severity Scale, Fatigue Impact Scale) and quality of life (Nottingham Heath Profile). Results: After the exercise programme, improvement was observed in the hospital exercise group compared to a pre-exercise period in all Nottingham Heath Profile scores (except sleep scores), pVo2, Fatigue Severity Scale and Fatigue Impact Scale (cognitive, physical, psychosocial, total) (P<0.05). In contrast, in the home exercise group a decrease was observed in pVo2 scores after the rehabilitation programme, compared to a pre-rehabilitation period (P<0.05). In addition, a significant improvement was observed in the home exercise group after the rehabilitation programme in all parameters excluding Fatigue Impact Scale—physical, Fatigue Impact Scale—psychosocial, and Nottingham Heath Profile—sleep (P<0.05). When the two exercise groups were compared, improvement was observed in the hospital exercise group compared to the home exercise group in pVo2 and Fatigue Severity Scale—total, Fatigue Impact Scale—physical, Fatigue Impact Scale—psychosocial, Fatigue Impact Scale—total, and Nottingham Heath Profile—energy scores (P<0.05). Conclusion: Fatigue and quality of life were both improved in the home and hospital exercise groups. An increase was also found in the functional capacity in the hospital exercise group. A regular exercise programme is beneficial to patients with post-polio syndrome.
Positive correlation between gene coexpression and positional clustering in the zebrafish genome
Authors: Ng Yen Kaow , Wu Wei , Zhang Louxin 
Published: January 2009

AbstractBackgroundCo-expressing genes tend to cluster in eukaryotic genomes. This paper analyzes correlation between the proximity of eukaryotic genes and their transcriptional expression pattern in the zebrafish (Danio rerio) genome using available microarray data and gene annotation.ResultsThe analyses show that neighbouring genes are significantly coexpressed in the zebrafish genome, and the coexpression level is influenced by the intergenic distance and transcription orientation. This fact is further supported by examining the coexpression level of genes within positional clusters in the neighbourhood model. There is a positive correlation between gene coexpression and positional clustering in the zebrafish genome.ConclusionThe study provides another piece of evidence for the hypothesis that coexpressed genes do cluster in the eukaryotic genomes.
The external validity of published randomized controlled trials in primary care
Authors: Jones Ritu , Jones Robert O , McCowan Colin , Montgomery Alan A , Fahey Tom 
Published: January 2009

Abstract

Background
A criticism of Randomized Controlled Trials (RCTs) in primary care is that they lack external validity, participants being unrepresentative of the wider population. Our aim was to determine whether published primary care-based RCTs report information about how the study sample is assembled, and whether this is associated with RCT characteristics.

Methods
We reviewed RCTs published in four primary care journals in the years 2001–2004. Main outcomes were: (1) eligibility fraction (proportion eligible of those screened), (2) enrolment fraction (proportion randomised of those eligible), (3) recruitment fraction (proportion of potential participants actually randomised), and (4) number of patients needed to be screened (NNS) in order to randomize one participant.

Results
A total of 148 RCTs were reviewed. One hundred and three trials (70%) reported the number of individuals assessed by investigators for eligibility, 119 (80%) reported the number eligible for participation, and all reported the actual number recruited. The median eligibility fraction was 83% (IQR 40% to 100%), and the median enrolment fraction was 74% (IQR 49% to 92%). The median NNS was 2.43, with some trials reportedly recruiting every patient or practice screened for eligibility, and one trial screening 484 for each patient recruited. We found no association between NNS and journal, trial size, multi- or single-centre, funding source or type of intervention. There may be associations between provision of sufficient recruitment data for the calculation of NNS and funding source and type of intervention.

Conclusion
RCTs reporting recruitment data in primary care suggest that once screened for eligibility and found to match inclusion criteria patients are likely to be randomized. This finding needs to be treated with caution as it may represent inadequate identification or reporting of the eligible population. A substantial minority of RCTs did not provide sufficient information about the patient recruitment process.
Atomic structures and functional implications of the archaeal RecQ-like helicase Hjm
Authors: Oyama Takuji , Oka Hayato , Mayanagi Kouta , Shirai Tsuyoshi , Matoba Kyoko , Fujikane Ryosuke , Ishino Yoshizumi , Morikawa Kosuke 
Published: January 2009

Abstract

Background

Pyrococcus furiosus Hjm (Pfu Hjm) is a structure-specific DNA helicase that was originally identified by in vitro screening for Holliday junction migration activity. It belongs to helicase superfamily 2, and shares homology with the human DNA polymerase Θ (PolΘ), HEL308, and Drosophila Mus308 proteins, which are involved in DNA repair. Previous biochemical and genetic analyses revealed that Pfu Hjm preferentially binds to fork-related Y-structured DNAs and unwinds their double-stranded regions, suggesting that this helicase is a functional counterpart of the bacterial RecQ helicase, which is essential for genome maintenance. Elucidation of the DNA unwinding and translocation mechanisms by Pfu Hjm will require its three-dimensional structure at atomic resolution.

Results
We determined the crystal structures of Pfu Hjm, in two apo-states and two nucleotide bound forms, at resolutions of 2.0–2.7 Å. The overall structures and the local conformations around the nucleotide binding sites are almost the same, including the side-chain conformations, irrespective of the nucleotide-binding states. The architecture of Hjm was similar to that of Archaeoglobus fulgidus Hel308 complexed with DNA. An Hjm-DNA complex model, constructed by fitting the five domains of Hjm onto the corresponding Hel308 domains, indicated that the interaction of Hjm with DNA is similar to that of Hel308. Notably, sulphate ions bound to Hjm lie on the putative DNA binding surfaces. Electron microscopic analysis of an Hjm-DNA complex revealed substantial flexibility of the double stranded region of DNA, presumably due to particularly weak protein-DNA interactions. Our present structures allowed reasonable homology model building of the helicase region of human PolΘ, indicating the strong conformational conservation between archaea and eukarya.

Conclusion
The detailed comparison between our DNA-free Pfu Hjm structure and the structure of Hel308 complexed with DNA suggests similar DNA unwinding and translocation mechanisms, which could be generalized to all of the members in the same family. Structural comparison also implied a minor rearrangement of the five domains during DNA unwinding reaction. The unexpected small contact between the DNA duplex region and the enzyme appears to be advantageous for processive helicase activity.
Antiphospholipid antibodies: Paradigm in transition
Authors: Horstman Lawrence L , Jy Wenche , Bidot Carlos J , Ahn Yeon S , Kelley Roger E , Zivadinov Robert , Maghzi Amir H , Etemadifar Masoud , Mousavi Seyed Ali , Minagar Alireza 
Published: January 2009

AbstractObjectivesThis is a critical review of anti-phospholipid antibodies (aPL). Most prior reviews focus on the aPL syndrome (APS), a thrombotic condition often marked by neurological disturbance. We bring to attention recent evidence that aPL may be equally relevant to non-thrombotic autoimmune conditions, notably, multiple sclerosis and ITP.OrganizationAfter a brief history, the recent proliferation of aPL target antigens is reviewed. The implication is that many more exist. Theories of aPL in thrombosis are then reviewed, concluding that all have merit but that aPL may have more diverse pathological consequences than now recognized. Next, conflicting results are explained by methodological differences. The lupus anticoagulant (LA) is then discussed. LA is the best predictor of thrombosis, but why this is true is not settled. Finally, aPL in non-thrombotic disorders is reviewed.ConclusionThe current paradigm of aPL holds that they are important in thrombosis, but they may have much wider clinical significance, possibly of special interest in neurology.
Multiple signaling pathways contribute to synergistic TLR ligand-dependent cytokine gene expression in human monocyte-derived macrophages and dendritic cells
Authors: Mäkelä Sanna M , Strengell Mari , Pietilä Taija E , Österlund Pamela , Julkunen Ilkka 
Published: January 2009

AbstractTLRs are innate immune receptors that recognize pathogen-associated structures. Binding of ligands to different TLRs can induce the production of proinflammatory cytokines in a synergistic manner. We have analyzed the molecular mechanisms of synergy in TLR ligand-stimulated human monocyte-derived macrophages and dendritic cells (moDCs). Stimulation of moDCs with the TLR8 ligand together with the TLR3 or TLR4 ligand led to synergistic IL-6, IL-10, IL-12, and TNF-α mRNA expression and cytokine production. DNA-binding assays showed that TLR3 and TLR8 stimulation induced binding of multiple IFN regulatory factor (IRF) and STAT transcription factors to the IL-12p35 gene promoter IFN-stimulated response element in moDCs and macrophages but with different binding profiles and kinetics. We also demonstrate that NF-κB, MAPKs and PI-3K pathways have an important role in TLR-induced cytokine gene expression, as pharmacological inhibitors of these signaling pathways inhibited TLR3, TLR4, and TLR8 ligand-induced cytokine mRNA expression and protein production. Especially, synergistic IL-12p70 production was abolished completely in NF-κB, MAPK p38, and PI-3K inhibitor-treated moDCs. Our data suggest that TLR-dependent, synergistic cytokine gene expression results from enhanced activation and cooperation among NF-κB, IRF, MAPK, PI-3K, and STAT signaling pathways.
Overcoming CYP1A1/1A2 Mediated Induction of Metabolism by Escalating Erlotinib Dose in Current Smokers
Authors: Hughes Andrew N. , O'Brien Mary E.R. , Petty W. Jeffrey , Chick Jonathan B. , Rankin Elaine , Woll Penella J. , Dunlop David , Nicolson Marianne , Boinpally Ramesh , Wolf Julie , Price Allan 
Published: January 2009

Purpose Cigarette smoking induces CYP1A1/1A2 and is hypothesized to alter erlotinib pharmacokinetics. This study aimed to determine the maximum tolerated dose (MTD) of erlotinib in advanced non–small-cell lung cancer (NSCLC) patients who smoke and compare the pharmacokinetics of erlotinib at the MTD in current smokers with 150 mg. Patients and Methods Cohorts of NSCLC patients currently smoking ≥ 10 cigarettes per day for ≥ 1 year received escalating doses of erlotinib for 14 days until dose-limiting toxicity (DLT). A separate cohort of patients was then randomly assigned to erlotinib at either MTD or 150 mg daily with pharmacokinetics assessed at day 14. Erlotinib was continued until progression or intolerable toxicity. Results Four dose levels were evaluated in 22 patients: 200, 250, 300, and 350 mg. DLT was observed in one of six patients at 300 mg (rash) and two of five patients at 350 mg (acneiform dermatitis and fatigue/decreased Eastern Cooperative Oncology Group performance status). Thirty-five patients were randomly assigned to 150 mg or 300 mg. Common adverse events (all grades) were: skin toxicity (150 mg, 29%; 300 mg, 67%), diarrhea (150 mg, 18%; 300 mg, 50%), and fatigue (150 mg, 12%; 300 mg, 17%). Erlotinib exposure was dose-proportional within dose range tested. Median steady-state trough erlotinib plasma concentrations were 0.375 and 1.22 μg/mL for 150 mg and 300 mg, respectively. Conclusion The MTD of erlotinib in NSCLC patients who smoke was 300 mg. Steady-state trough plasma concentrations and incidence of rash and diarrhea in smokers at 300 mg were similar to those in former or never smokers receiving 150 mg in previous studies. The potential benefit of higher erlotinib doses in current smokers warrants further evaluation.