bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oligodendrocyte differentiation is increased in transferrin transgenic mice
Authors: Sow Amina , Lamant Matthieu , Bonny Jean‐Marie , Larvaron Pierre , Piaud Oriane , Lécureuil Charlotte , Fontaine Isabelle , Saleh Maria‐Carla , Garcia Otin Angel Luis , Renou Jean‐Pierre , Baron Bruno , Zakin Mario , Guillou Florian 
Published: January 2006

AbstractTransferrin (Tf), the iron transport glycoprotein found in biological fluids of vertebrates, is synthesized mainly by hepatocytes. Tf is also synthesized by oligodendrocytes (Ol), and several lines of evidence indicate that brain Tf could be involved in myelinogenesis. Because Tf is postnatally expressed in the brain, we sought to investigate whether Tf could intervene in Ol differentiation. For this purpose, we analyzed transgenic mice overexpressing the complete human Tf gene in Ol. We show that the hTf transgene was expressed only from 5 days postpartum onward. In the brain of 14‐day‐old transgenic mice, the DM‐20 mRNA level was decreased, whereas the PLP, MBP, CNP, and MAG mRNA levels were increased. We counted a higher proportion of Ol expressing the O4 (Ol‐specific antigens) and PLP in brain cells cultured from transgenic mice. These results support the idea that overexpressing Tf in the brain accelerates the oligodendrocyte lineage maturation. Accordingly, by NMR imaging acquisition of diffusion tensor in hTf transgenic mice, we observed early maturation of the cerebellum and spinal cord and more myelination in the corpus callosum. In addition, hTf overexpression led to an increase in Sox10 mRNA and protein. Increases in Sox10 and in Tf expression occur simultaneously during brain development. The Olig1 mRNA level also increased, but long after the rise of hTf and Sox10. The Olig2 mRNA level remained unchanged in the brain of transgenic mice. Our findings suggest that Tf could influence oligodendrocyte progenitor differentiation in the CNS. © 2006 Wiley‐Liss, Inc.
Human immunodeficiency virus type 1 gp120 inhibits long‐term potentiation via chemokine receptor CXCR4 in rat hippocampal slices
Authors: Dong Jun , Xiong Huangui 
Published: January 2006

AbstractHuman immunodeficiency virus type 1 (HIV‐1) infection in its human host often results in progressive dementia and encephalopathy in adults and children, respectively. The mechanisms underlying virus‐induced neurocognitive dysfunction are not fully understood. However, several studies strongly suggest that secretory viral and immune products from infected brain macrophages and microglia affect the onset and tempo of disease. One critical neurotoxin among these secretory products is the HIV‐1 envelope glycoprotein gp120. To better understand how HIV‐1 gp120 may affect cognitive function, we studied its effects on long‐term potentiation (LTP) in the CA1 region of rat hippocampus, the brain region best linked to learning and memory. Although no effects were observed on basal synaptic transmission, HIV‐1 gp120 inhibited LTP in a concentration‐dependent manner in the presence of γ‐aminobutyric acid type A (GABAA) receptor antagonist. Heat‐inactivated gp120 failed to block LTP. The HIV‐1 gp120‐mediated LTP inhibition was blocked by T140, a chemokine receptor CXCR4 antagonist, demonstrating gp120 inhibition of LTP via CXCR4. HIV‐1 gp120 V3 loop peptides mimicked the inhibitory effects of HIV‐1 gp120 protein on LTP. Monoclonal antibodies against the V3 loop epitope KRIHI eliminated the HIV‐1 gp120 effects on LTP. These results further underscore the importance of HIV‐1 gp120 in the pathogenesis of HIV‐1‐associated cognitive impairments seen during progressive viral infection. © 2006 Wiley‐Liss, Inc.
Gender differences in hip adduction motion and torque during a single‐leg agility maneuver
Authors: Hewett Timothy E. , Ford Kevin R. , Myer Gregory D. , Wanstrath Kim , Scheper Melia 
Published: January 2006

AbstractThe purpose of this study was to identify gender differences in hip motion and kinetics during a single leg bidirectional deceleration maneuver. The rationale for the development of this maneuver was to test dynamic hip control during the deceleration of three different types of single‐leg landings. The hypothesis was that female athletes would display increased hip adduction angles and moments during the maneuver compared to male athletes. Thirty‐six collegiate soccer players (19 female, 17 male) volunteered to participate. Subjects were instructed to start the maneuver balancing on one foot, to hop through an agility‐speed ladder on the same leg “up two boxes, back one, and then up one and hold it.” Hip kinematics and kinetics during all three landings were examined. Females demonstrated significantly greater hip adduction angles at initial contact during all three landings and greater maximal hip adduction during landings 1 and 2 compared to male athletes. Females also exhibited significantly increased external hip adduction moments during landing 1, however, no differences were found between genders during landings 2 and 3. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:416–421, 2006
Antibiotic‐containing hyaluronic acid gel as an antibacterial carrier: Usefulness of sponge and film‐formed HA gel in deep infection
Authors: Matsuno Hiroaki , Yudoh Kazuo , Hashimoto Masamichi , Himeda Yasukazu , Miyoshi Teruzo , Yoshida Kaoru , Kano Syogo 
Published: January 2006

AbstractWe have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:321–326, 2006
Genomic instabilities in squamous cell carcinoma of head and neck from the Indian population
Authors: Chakrabarti Susmita , Sengupta Shiladitya , Sengupta Arunava , Basak Saminendra Nath , Roy Anup , Panda Chinmay , Roychoudhury Susanta 
Published: January 2006

AbstractIntrinsic genomic instability of an incipient tumor cell drives the development of cancer. In colorectal cancer, an inverse relationship between microsatellite instability (MIN) and chromosomal instability (CIN) has been reported. The relationship between MIN and CIN in head and neck squamous cell carcinoma (HNSCC) is uncertain. In the present study, we examined these two types of instabilities in HNSCC using microsatellite markers and arbitrary primed PCR (APPCR) technique. HNSCC tumors showed high frequency of MIN and loss of heterozygosity (LOH). We observed that, in contrast to colorectal tumors, the frequency of LOH increased with the increase in MIN. There was no significant difference between MIN− and MIN+ groups of HNSCC tumors in the extent of overall genomic alterations; rather higher MIN+ tumors exhibited higher incidence of deletion. Thus, in sporadic HNSCC, both MIN and CIN pathways operate simultaneously to drive tumor formation. © 2006 Wiley‐Liss, Inc.
Lysophosphatidic acid stimulates PC‐3 prostate cancer cell matrigel invasion through activation of RhoA and NF‐κB activity
Authors: Hwang Young Sun , Hodge Jennelle C. , Sivapurapu Neela , Lindholm Paul F. 
Published: January 2006

AbstractThis study was performed to determine the relationship of lysophosphatidic acid (LPA) stimulation and increased Ras homolog A (RhoA) activity to nuclear factor kappa B (NF‐κB) activity, and the role of these factors in regulating prostate cancer cell invasion. PC‐3 high invasive cells demonstrated constitutively increased RhoA, NF‐κB, and in vitro Matrigel invasion which were further induced by LPA stimulation or transfection with constitutively active RhoA Q63E mutant. LPA treatment rapidly and transiently induced RhoA activity followed by maximally increased DNA binding of NF‐κB at 1 h and AP‐1 at 4 h. The LPA‐induced NF‐κB DNA binding was preceded by transient IκBα phosphorylation, and decreased total IκBα levels. Further demonstrating the relationship between RhoA and NF‐κB activation, PC‐3 cells stably transfected with constitutively active RhoA Q63E demonstrated constitutively increased phospho‐IκBα, while PC‐3 cells transfected with dominant negative RhoA N19 exhibited decreased phospho‐IκBα levels. The LPA‐induced Matrigel invasion and NF‐κB DNA binding activity were both inhibited by expression of the RhoA inhibitor C3 exoenzyme or dominant negative mutant NF‐κB inhibitor IκBα S32/36A. Similarly, transfection with dominant negative IκBα S32/36A inhibited PC‐3 RhoA Q63E cell in vitro invasion. Treatment of PC‐3 high invasive and RhoA Q63E cells with sodium salicylate or lactacystin inhibited NF‐κB and invasion, while pyrrolidine dithiocarbamate (PDTC) treatment of PC‐3 high invasive cells inhibited NF‐κB only. Each inhibitor blocked LPA‐induced invasion while PDTC inhibited LPA‐induced NF‐κB and invasion to the greatest extent. These results point to a model where LPA stimulates RhoA and increased PC‐3 prostate cancer cell invasion activity through an NF‐κB‐dependent pathway. © 2006 Wiley‐Liss, Inc.
Comparative expressed sequence hybridization reveals differential gene expression in morphological breast cancer subtypes
Authors: Vanden Bempt Isabelle , Vanhentenrijk Vera , Drijkoningen Maria , Wolf‐Peeters Christiane De 
Published: January 2006

AbstractIn this study, comparative expressed sequence hybridization (CESH) has been used to compare gene expression patterns in three morphologically different breast cancer subtypes: classic‐type invasive lobular carcinoma (ILC), poorly differentiated ERBB2‐negative invasive ductal carcinoma‐not otherwise specified (IDC‐NOS), and poorly differentiated ERBB2‐positive IDC‐NOS. CESH allows global detection of chromosomal regions with differential gene expression in a way similar to that of comparative genomic hybridization (CGH). Eight cases of each breast cancer subtype were included in the study. For each subtype, two pools of four cases each were constructed. CESH was used to compare both pools within the same morphological subtype, followed by a comparison of pools belonging to different subtypes. This revealed three chromosomal regions that were differentially expressed in ductal and lobular carcinomas, including relative overexpression at 8q13–q23 and 16q22, and relative underexpression at 8p21–p22. In addition, an expression signature characterized by relative overexpression at 3q24–q26.3, 14q23–31, 17q12, and 20q12–13 was identified for ERBB2‐positive IDC‐NOS. In summary, CESH analysis highlights chromosomal regions of differential gene expression that are associated with morphologically defined breast cancer subtypes and suggests that regions on chromosome 8 are of interest in the discrimination between ductal and lobular carcinomas. In addition, using CESH, it was possible to identify an ERBB2 expression signature, comprising four chromosomal regions with potential significance in the aggressive behaviour of ERBB2‐positive IDC‐NOS. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Comprehensive genomic analysis of desmoplastic medulloblastomas: identification of novel amplified genes and separate evaluation of the different histological components
Authors: Ehrbrecht A , Müller U , Wolter M , Hoischen A , Koch A , Radlwimmer B , Actor B , Mincheva A , Pietsch T , Lichter P , Reifenberger G , Weber RG 
Published: January 2006

AbstractDesmoplastic medulloblastoma (DMB) is a malignant cerebellar tumour composed of two distinct tissue components, pale islands and desmoplastic areas. Previous studies revealed mutations in genes encoding members of the sonic hedgehog pathway, including PTCH, SMOH and SUFUH in DMBs. However, little is known about other genomic aberrations. We performed comparative genomic hybridization (CGH) analysis of 22 sporadic DMBs and identified chromosomal imbalances in 20 tumours (91%; mean, 4.9 imbalances/tumour). Recurrent chromosomal gains were found on chromosomes 3, 9 (six tumours each), 20, 22 (five tumours each), 2, 6, 7, 17 (four tumours each) and 1 (three tumours). Recurrent losses involved chromosomes X (eight tumours), Y (six of eleven tumours from male patients), 9, 12 (four tumours each), as well as 10, 13 and 17 (three tumours each). Four tumours demonstrated high‐level amplifications involving sequences from 1p22, 5p15, 9p, 12p13, 13q33‐q34 and 17q22‐q24, respectively. Further analysis of the 9p and 17q22‐q24 amplicons by array‐based CGH (matrix‐CGH) and candidate gene analyses revealed amplification of JMJD2C at 9p24 in one DMB and amplification of RPS6KB1, APPBP2, PPM1D and BCAS3 from 17q23 in three DMBs. Among the 17q23 genes, RPS6KB1 showed markedly elevated transcript levels as compared to normal cerebellum in five of six DMBs and four of five classic medulloblastomas investigated. Finally, CGH analysis of microdissected pale islands and desmoplastic areas showed common chromosomal imbalances in five of six informative tumours. In summary, we have identified several novel genetic alterations in DMBs and provide genetic evidence for a monoclonal origin of their different tissue components. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Practice development and action learning
Authors: Edmonstone John , MacKenzie Hazel 
Published: January 2006

AbstractThis article seeks to explore the potentially fruitful connections between practice development and the approach to personal and organizational development known as action learning. Action learning is defined as: a method for individual and organization development based upon small groups of colleagues meeting over time to tackle real problems or issues in order to get things done; reflecting and learning from their experience and from each other as they attempt to change things (Edmonstone, 2003). The article begins by examining the nature of professional practice and the relationship between theory and practice. It then considers the nature of learning, including an examination of competence and capacity. It reviews action learning and its underlying roots in adult learning, focusing particularly on the concentration of action learning on ‘problems’ rather than ‘puzzles’. It examines the nature of practice development and then explores the relationship between practice development and action learning through material derived from an evaluation study conducted on the Royal College of Nursing Clinical Leadership Programme in Scotland where action learning forms a major element of the programme. Copyright © 2005 Whurr Publishers Ltd.
Practice development: ‘plausibility’, ‘doability’ and ‘outcome’ issues
Authors: Carr Susan M 
Published: January 2006

AbstractThere is a dearth of literature exposing the systems, processes and roles involved in practice development activity. This paper addresses this deficit by proposing a framework derived from research to facilitate setting the aim and enabling the process of practice development. Based on the theories of change approach, the context of the development is a key component of the process. The method of articulating the rationale guiding the activity ensures that it is both ‘plausible’ and ‘doable’ thereby enhancing the likelihood of a successful outcome. Copyright © 2005 Whurr Publishers Ltd.
Proteomic analysis of cellular change involved in mitochondria‐to‐nucleus communication in L6 GLUT4myc myocytes
Authors: Park Seung Yoon , Lee Sanghyun , Park Kyong Soo , Lee Hong Kyu , Lee Wan 
Published: January 2006

AbstractGenetic or biochemical abnormalities in mitochondria are closely associated with apoptosis, aging, cancer, and other chronic degenerative diseases. Mitochondrial dysfunction resulting from mitochondrial DNA (mtDNA) depletion dispatches retrograde signals to the nucleus to compensate by altering the expression of various genes. In this study, a proteomic approach was used to gain insight into the nuclear gene targets of mitochondrial stress signaling and the pathophysiological mechanisms associated with mitochondrial dysfunction. We have used 2‐DE to characterize the nuclear gene responses resulting from mtDNA depletion in L6 GLUT4myc myocytes. Our results showed that 77 polypeptides were differentially expressed in mtDNA‐depleted cells; 33 polypeptides were down‐regulated and 44 polypeptides were up‐regulated. Of these differentially expressed polypeptides, 40 were identified as 36 different proteins by MALDI‐TOF MS. These proteins are related to various cellular responses, such as apoptosis, cellular metabolism, signaling and cytoskeleton functions. It is suggested that the insulin resistance developed in mtDNA‐depleted myocytes may be associated with disorganization of cytoskeleton assembly, and that cellular mtDNA depletion might promote the ability to evade apoptosis or other death effectors.
High‐throughput analysis of GST‐fusion protein expression and activity‐dependent protein interactions on GST‐fusion protein arrays with a spectral surface plasmon resonance biosensor
Authors: Jung Jae‐Wan , Jung Se‐Hui , Kim Hyun‐Soo , Yuk Jong Seol , Park Jae‐Bong , Kim Young‐Myeong , Han Jeong‐A , Kim Pyung‐Hyun , Ha Kwon‐Soo 
Published: January 2006

AbstractWe modified gold arrays with a glutathione (GSH) surface, and investigated high‐throughput protein interactions with a spectral surface plasmon resonance (SPR) biosensor. We fabricated the GSH exterior on gold surfaces by successive modification with aminoethanethiol, 4‐maleimidobutyric acid N‐hydroxysuccinimide ester and GSH. We immobilized GST‐Rac1, GST‐RhoA, the GST‐Rho‐binding domain of rhotekin and the GST‐p21‐binding domain of PAK1 onto the GSH surface, and observed specific antigen‐antibody interactions on the GST‐fusion protein arrays. We determined the expression of GST‐fusion proteins in Escherichia coli on the GSH surface with the SPR biosensor. We then analyzed the interactions of tissue transglutaminase (tTGase), a Ca2+‐dependent enzyme, with RhoA and Rac1 on the GST‐fusion protein arrays with the SPR biosensor. We found that tTGase interacted with RhoA and Rac1 in a Ca2+‐dependent manner, indicating that the interactions were dependent on tTGase activity. In addition, transamidation of Rac1 by tTGase was dependent on Ca2+ concentration. We obtained similar results with GST pull‐down assays. Thus, protein arrays prepared on the GSH surface provide a useful system for the high‐throughput analysis of GST‐fusion protein expression and activity‐dependent protein interactions with the spectral SPR biosensors.
Loss of transgelin in repeated bouts of ulcerative colitis‐induced colon carcinogenesis
Authors: Yeo Marie , Kim Dong‐Kyu , Park Hee Jin , Oh Tae Young , Kim Jang Hee , Cho Sung Won , Paik Young‐Ki , Hahm Ki‐Baik 
Published: January 2006

AbstractThough ulcerative colitis (UC)‐associated colon cancer develops from dysplastic lesions caused by chronic inflammation, the specific mechanistic link between chronic inflammation and carcinogenesis in colon has not been integrated into molecular understanding. We therefore established an experimental animal model for colitic cancer, and used proteomic analysis, based on 2‐DE and MALDI‐TOF MS, to identify proteins involved in colitic cancer. In our model, 6‐week‐old C57BL/6J mice were exposed to 15 cycles of dextran sodium sulfate (DSS), with each cycle consisting of 0.7% DSS for 1 week followed by distilled water for 10 days. Colorectal tumors developed in 22 of 24 mice (91.6%), with a tumor multiplicity of 1.727 per tumor‐bearing mouse. Comparative 2‐DE analysis showed that 38 protein spots were differentially expressed in colon tumors and normal colon. We identified 27 of these proteins, including GRP94, HSC70, enolase, prohibitin, and transgelin. The reduction of transgelin expression in mouse colon tumors was confirmed by Western blotting and immunohistochemistry. We also found that transgelin expression was significantly reduced in human colon tumors compared with adjacent nontumorous tissues. In conclusion, these results suggest that loss of transgelin could be a candidate for biomarker of repeated colitis‐associated colon cancer.
Human Disease Glycomics/Proteome Initiative Workshop and the 4th HUPO Annual Congress
Authors: Taniguchi Naoyuki , Nakamura Kazuyuki , Narimatsu Hisashi , von der Lieth Claus‐Willi , Paulson James 
Published: January 2006

AbstractAt the Human Disease Glycomics/Proteome Initiative workshop held on August 27 on the occasion of the fourth HUPO Congress in Munich, Germany, the working groups reported on pilot studies, performed by 21 laboratories world‐wide, to analyze glycan using MS. During the steering committee on July 31 in Osaka, Japan, it was reported that these groups have developed a system using MS to assess the world‐wide network on the screening of congenital disorders of glycosylation. The concept of glycobioinformatics was also explained at this meeting. The session on PTMs laid particular emphasis on the importance of functional glycomics using glycosyltransferase genes as well as on the importance of identifying the target protein(s) carrying the sugar chains.
Guidelines for the next 10 years of proteomics
Authors: Wilkins Marc R. , Appel Ron D. , Van Eyk Jennifer E. , Chung Maxey C. M. , Görg Angelika , Hecker Michael , Huber Lukas A. , Langen Hanno , Link Andrew J. , Paik Young‐Ki , Patterson Scott D. , Pennington Stephen R. , Rabilloud Thierry , Simpson Richard J. , Weiss Walter , Dunn Michael J. 
Published: January 2006

AbstractIn the last ten years, the field of proteomics has expanded at a rapid rate. A range of exciting new technology has been developed and enthusiastically applied to an enormous variety of biological questions. However, the degree of stringency required in proteomic data generation and analysis appears to have been underestimated. As a result, there are likely to be numerous published findings that are of questionable quality, requiring further confirmation and/or validation. This manuscript outlines a number of key issues in proteomic research, including those associated with experimental design, differential display and biomarker discovery, protein identification and analytical incompleteness. In an effort to set a standard that reflects current thinking on the necessary and desirable characteristics of publishable manuscripts in the field, a minimal set of guidelines for proteomics research is then described. These guidelines will serve as a set of criteria which editors of PROTEOMICS will use for assessment of future submissions to the Journal.