bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo effects of interleukin‐1 on articular cartilage. Prolongation of proteoglycan metabolic disturbances in old mice
Authors: van Beuningen Henk M. , Arntz Onno J. , Van den Berg Wim B. 
Published: February 2007

AbstractWe investigated the effects of intraarticular injections of interleukin‐1α (IL‐1α) into the knee joints of young (3‐month‐old) and old (18‐month‐old) C57B1/10 mice. In this in vivo study, 35S‐sulfate incorporation and release were used to compare the effects of IL‐1 on patellar cartilage proteoglycan metabolism. IL‐1–induced stimulation of proteoglycan degradation was confined to the first 24 hours after injection in both young and old animals, and was only slightly lower in old cartilage than in young. In old patellar cartilage, IL‐1–induced suppression of proteoglycan synthesis appeared to be more prolonged. Also, the amount of time needed to restore the cartilage matrix, characterized by proteoglycan synthesis above normal levels, was longer in old animals. Histologic analysis confirmed the retarded recovery in the cartilage of old mice. Autoradiography showed that the chondrocytes of the medial side of the femorotibial area were most vulnerable to IL‐1–induced suppression of proteoglycan synthesis, especially the medial tibial plateau. As with the patellar cartilage, IL‐1–induced suppression of proteoglycan synthesis in this area of articular cartilage was more prolonged in old animals. Our data indicate that the impact of IL‐1 on articular cartilage is higher in old mice and that, consistent with certain loci found to be at risk in experimental osteoarthritis, there are sites at which IL‐1 is more likely to act.
The progression of erosion and joint space narrowing scores in rheumatoid arthritis during the first twenty‐five years of disease
Authors: Sharp John T. , Wolfe Frederick , Mitchell Donald M. , Bloch Daniel A. 
Published: February 2007

AbstractErosions and cartilage destruction are nearly universal features in peripheral joints that have been chronically affected by rheumatoid arthritis. Scoring methods to measure the extent of these abnormalities in hands and wrists have been developed and have been thoroughly tested in several studies to establish their reproducibility. In this study, we utilized one of these scoring methods to examine the progression of radiologic damage as related to duration of disease. Two hundred ninety‐two patients from 3 different participating centers in the Arthritis, Rheumatism, and Aging Medical Information System were included. Six hundred fifty films of the hands and wrists, obtained from 210 patients, were scored for erosions and joint space narrowing. The average annual rate of progression of the total radiologic score, which sums erosion and joint space abnormalities and has a maximum possible score of 314, was approximately 4 units per year over the first 25 years after onset; this progression was more rapid in the earlier years of disease and slightly slower in the later years. Data were insufficient to accurately determine the progression rate in disease of more than 25 years duration.
Sera from patients with autoimmune disease recognize conformational determinants on the 60‐kd ro/ss‐a protein
Authors: Boire Gilles , Lopez‐Longo Francisco‐Javier , Lapointe Sylvie , Ménard Henri‐A. 
Published: February 2007

AbstractAnti‐Ro antibodies are found in a large proportion of patients with systemic lupus erythematosus and primary Sjögren's syndrome. These antibodies also characterize neonatal lupus, subacute cutaneous lupus erythematosus, and vasculitis associated with Sjögren's syndrome and rheumatoid arthritis. Anti‐Ro‐positive sera may contain either or both of 2 sets of antibodies, recognizing either a 60‐kd or a 52‐kd polypeptide component of the Ro particle. We found in this study that the immune response to the 60‐kd Ro antigen is heterogeneous. Some sera specifically recognize the native Ro antigen but fail to bind the corresponding denatured polypeptides. In addition, after immunodepletion using the denatured 60‐kd Ro polypeptide, all anti‐Ro‐positive sera tested still contained high titers of antibodies recognizing conformational determinants on the Ro antigen. The frequent immunodominance of anti‐Ro antibodies targeted to conformational determinants suggests that native autoantigens may directly drive the autoimmune response.
Shoulder disorders in the elderly: A community survey
Authors: Chard M. D. , Hazleman R. , Hazleman B. L. , King R. H. , Reiss B. B. 
Published: February 2007

AbstractA community survey of identifiable symptomatic shoulder disorders in a sample of 644 elderly people over age 70 (318 male and 326 female) revealed a prevalence of 21%. Shoulder disorders were more common in women (25%, versus 17% in men). Approximately 70% of the cases of shoulder pain involved the rotator cuff. Fewer than 40% of the subjects sought medical attention for these symptoms. Increased medical awareness is needed, since the elderly often do not volunteer information about such symptoms.
The latex test revisited. Rheumatoid factor testing in 8,287 rheumatic disease patients
Authors: Wolfe Frederick , Cathey Mary Ann , Roberts F. Kay 
Published: February 2007

AbstractRheumatoid factor (RF) testing by latex fixation in 8,287 outpatients yielded a sensitivity of 81.6% and 78.0% at titers of 1:20 and 1:80, respectively, and a specificity against noninflammatory rheumatic disorders (NIRD) of 96.6% and 97.9% and against NIRD plus inflammatory disorders of 95.2% and 96.8%, respectively. The predictive value of a positive test result at the clinic prevalence rate for rheumatoid arthritis (RA) (16.4%) was approximately 80%, and was 70% at 10% prevalence and 10% at 1% prevalence. No associations of RF with age or sex were found in non‐RA patients. RF titers increased minimally with age in RA patients and were higher in men than in women. This study suggests that latex testing is far more specific than has been believed and that the titer is not spuriously increased with age.
Long‐term colchicine treatment in children with familial mediterranean fever
Authors: Zemer Deborah , Livneh Avi , Danon Yehuda L. , Pras Mordechai , Sohar Ezra 
Published: February 2007

AbstractThree hundred fifty children (younger than age 16) who had familial Mediterranean fever (FMF) were given continuous prophylactic treatment with colchicine (1–2 mg/day) for 6–13 years. Complete remission of febrile attacks was achieved in 64% of the patients, and partial remission in 31%. Protracted attacks of arthritis virtually disappeared. None of the children developed amyloidosis while on the colchicine regimen. Side effects of colchicine were insignificant, and did not prompt permanent discontinuation of treatment in any of the children. Their growth, development, and subsequent fertility were normal. The efficacy of long‐term colchicine treatment of children with FMF makes early diagnosis life saving.
In Situ Hybridization Studies of Stromelysin and Collagenase Messenger RNA Expression in Rheumatoid Synovium
Authors: Gravallese Ellen M. , Darling Jama M. , Ladd Amy L. , Katz Jeffrey N. , Glimcher Laurie H. 
Published: February 2007

AbstractDestructive joint changes in rheumatoid arthritis (RA) are thought to be mediated in part by the neutral proteinases collagenase and stromelysin. Collagenase messenger RNA (mRNA) has been previously localized to the synovial lining layer. In this study, synovial tissue from 8 patients with RA and 2 patients with osteoarthritis was examined for proteinase production by in situ hybridization. Stromelysin mRNA localized predominantly to the synovial lining layer cells. In serial sections, collagenase mRNA was shown to be localized to the same tissue areas as those producing stromelysin mRNA, and grain counts revealed a direct correlation between production of stromelysin mRNA and production of collagenase mRNA. All patients with RA were producing collagenase and stromelysin mRNA in detectable amounts. One of 2 osteoarthritis patients was producing these metalloproteinases, but in levels below those found in the RA patients. These data support the identity of the synovial lining cells as the major synovial cells producing collagenase and stromelysin in RA and provide new evidence for the coordinate production of collagenase and stromelysin in RA in vivo.
Human Synovial Mast Cell Involvement in Rheumatoid Arthritis and Osteoarthritis. Relationship to Disease Type, Clinical Activity, and Antirheumatic Therapy
Authors: Bridges Alan J. , Malone Daniel G. , Jicinsky Judy , Chen Marcus , Ory Peter , Engber William , Graziano Frank M. 
Published: February 2007

AbstractMast cells were isolated by enzymatic digestion of synovium obtained from 48 patients with rheumatoid arthritis (RA) and 42 patients with osteoarthritis (OA). A significantly lower percentage of stainable synovial mast cells was obtained by tissue digestion from patients with clinically active RA compared with those with less active disease. The 54 patients treated with nonsteroidal antiinflammatory drugs had a significantly lower percentage of stainable synovial mast cells in cell suspension than did the other 36 patients. When anti‐IgE antibody was used as a secretagogue in vitro, significantly greater histamine release was observed from synovial mast cells of RA patients compared with OA patients. Greater histamine release in response to anti‐IgE was observed in the RA patients with more clinically active disease and those who were treated with prednisone, compared with RA patients without these features. Synovial mast cells of RA patients treated with a disease‐modifying antirheumatic drug had a significantly lower mean histamine content than did cells from patients not receiving such treatment. Our data suggest that there are differences between synovial mast cells from tissues of patients with RA and OA and suggest that synovial mast cells may be activated in clinically active RA. In addition, the data indicate an effect of systemic anti‐rheumatic therapy on mast cells isolated from synovium of patients with arthritis.
Expression and Function of Surface Antigens on Scleroderma Fibroblasts
Authors: Abraham D. , Lupoli S. , McWhirter A. , Plater‐Zyberk C. , Piela T. H. , Korn J. H. , Olsen Irwin , Black C. 
Published: February 2007

AbstractDermal fibroblasts from patients with systemic sclerosis (SSc) bound a much greater number of T lymphocytes than did normal dermal fibroblasts. Monoclonal antibodies (MAb) against classes I and II antigens of the major histocompatibility complex (MHC) and their receptors, CD8 and CD4, had no effect on T cell interaction with SSc and normal cells, while MAb against lymphocyte function–associated antigen type 3 (LFA‐3) and CD2 both strongly inhibited lymphocyte attachment. MAb against intercellular adhesion molecule type 1 (ICAM‐1) and LFA‐1 also prevented binding of T lymphocytes, but had a more marked effect on adhesion to SSc fibroblasts than to normal fibroblasts; they also completely abolished the increased binding to fibroblasts treated with interleukin‐1α, tumor necrosis factor α, and interferon‐γ. No difference was found in the proportion of normal and SSc fibroblasts that expressed MHC classes I and II and LFA‐3, but more SSc cells expressed ICAM‐1, and at a higher level, than did normal fibroblasts. These results show that cultured SSc cells have elevated binding to T lymphocytes, which possibly results from expansion of a subset of fibroblasts that produces high levels of ICAM‐1.
Effects of Body Weight Restriction on the Development and Progression of Spontaneous Osteoarthritis in Guinea Pigs
Authors: Bendele Alison M. , Hulman James F. 
Published: February 2007

AbstractHartley albino guinea pigs develop spontaneous osteoarthritis (OA) of the knee joint. A study was done to determine the importance of body weight in the pathogenesis of this disease. Two groups of 20 male guinea pigs each were maintained on the same diets. The control group was allowed ad libitum feed consumption and the other group was restricted to 30–35 gm of feed per day. Ten animals from each group were killed at 9 months of age to evaluate histologic features of the knee joints. The severity of the OA lesions was reduced by 40%, in conjunction with a 28% decrease in body weight, in the diet‐restricted group. The remaining animals were killed at 18 months of age. Those in the diet‐restricted group had a 56% reduction in severity of lesions, with a 29% decrease in body weight. These results indicate that body mass in guinea pigs, as in humans, is an important predisposing factor for the development of spontaneous OA of the knee.
The European Spondylarthropathy Study Group Preliminary Criteria for the Classification of Spondylarthropathy
Authors: Dougados Maxime , Linden Sjef Van Der , Juhlin Roger , Huitfeldt Bernhard , Amor Bernard , Calin Andrei , Cats Arnold , Dijkmans Ben , Olivieri Ignazio , Pasero Giampiero , Veys Eric , Zeidler Henning 
Published: February 2007

AbstractClassification criteria for most of the disorders belonging to the spondylarthropathy group already exist. However, the spectrum of spondylarthropathy is wider than the sum of these disorders suggests. Sero‐negative oligoarthritis, dactylitis or polyarthritis of the lower extremities, heel pain due to enthesitis, and other undifferentiated cases of spondylarthropathy have been ignored in epidemiologic studies because of the inadequacy of existing criteria. In order to define classification criteria that also encompass patients with undifferentiated spondylarthropathy, we studied 403 patients with all forms of spondylarthropathy and 674 control patients with other rheumatic diseases. The diagnoses were based on the local clinical expert's opinion. The 403 patients included 168 with ankylosing spondylitis, 68 with psoriatic arthritis, 41 with reactive arthritis, 17 with inflammatory bowel disease and arthritis, and 109 with unclassified spondylarthropathy. Based on statiscal analysis and clinical reasoning, we propose the following classification criteria for spondylarthropathy inflammatory spinal pain or synovitis (asymmetric or predominantly in the lower limbs), together with at least 1 of the following: positive family history, psoriasis inflammatory bowel disease, urethritis, or acute diarrhea, alternating buttock pain, enthesopathy, or sacroiliitis as determined from radiography of the pelvic region. These criteria resulted in a sensitivity of 87% and a specificity of 87%. The proposed classification criteria are easy to apply in clinical practice and performed well in all 7 participating centers. However, we regard them as preliminary until they have been further evaluated in other settings.
Kidney Biopsy in Systemic Lupus Erythematosus. II. Survival Analyses According to Biopsy Results
Authors: McLaughlin John , Gladman Dafna D. , Urowitz Murray B. , Bombardier Claire , Farewell Vernon T. , Cole Edward 
Published: February 2007

AbstractRenal biopsy specimens from 123 patients with systemic lupus erythematosus (SLE) seen between 1970 and 1984 were assessed according to the World Health Organization classification and according to the presence of proliferative, active, or chronic renal lesions. Survival analysis was used to study the determinants of mortality. Survival rates were higher for patients with minimal lesions, intermediate for patients with focal or diffuse proliferative nephritis, and low for patients with glomerular sclerosis. The presence of proliferative and chronic lesions was associated with a higher risk of dying. Renal biopsy results are helpful in predicting prognosis for all‐cause mortality in patients with SLE.
Effect of an Extract of the Chinese Herbal Remedy Tripterygium Wilfordii Hook F on Human Immune Responsiveness
Authors: Tao Xuelian , Davis Laurie S. , Lipsky Peter E. 
Published: February 2007

AbstractTripterygium wilfordii Hook F (TWH) is a vine‐like plant that grows in a wide area of south China. An alcohol extract of this plant known as T2 has been suggested to be effective in the treatment of rheumatoid arthritis (RA). To examine the mechanism by which this herbal remedy might be effective in RA, the capacity of T2 to alter human immune responsiveness in vitro was investigated. Human peripheral blood mononuclear cells were obtained from normal adults and separated into purified populations of monocytes, T cells, and B cells. T2 at 0.1–1 μg/ml inhibited antigen‐ and mitogen‐stimulated proliferation of T cells and B cells, interleukin‐2 (IL‐2) production by T cells, and immunoglobulin production by B cells. T2 did not affect IL‐2 receptor expression by T cells, IL‐1 production by monocytes, or the capacity of monocytes to present antigen. Inhibition could not be accounted for by nonspecific toxicity. These results support the conclusion that T2 exerts a powerful suppressive effect on human immune responses. This action might account for its therapeutic effectiveness in RA.
Fibromyalgia and Parvovirus Infection
Authors: Leventhal Lawrence J. , Naides Stanley J. , Freundlich Bruce 
Published: February 2007

AbstractAn infectious cause of fibromyalgia (FM) has been hypothesized based upon the observed similarity of this entity and chronic fatigue syndrome. Three patients developed symptoms of FM after documented episodes of acute parvovirus B19 infections. B19 antibody determinations were obtained approximately 1 month after the symptoms began; both IgM and IgG titers were positive at that time. All 3 patients met criteria for FM. Polysomnography performed on 2 of the patients revealed profound alpha‐wave intrusion throughout non–rapid eye movement sleep. A more careful search for viral infections in FM patients whose symptoms appear following a „flu‐like”︁ illness appears warranted.
A Case of Sjögren's Syndrome With Repeatedly Negative Findings on Lip Biopsy
Authors: Katz James , Yamase Harold , Parke Ann 
Published: February 2007

AbstractWe report a case of Sjögren's syndrome characterized by keratoconjunctivitis sicca, parotid swelling, reduced salivary flow, and abnormalities on nuclear scanning. Two labial gland biopsies showed complete atrophy. Biopsy of an enlarged submandibular gland revealed lymphoid follicles and glandular replacement consistent with the diagnosis of Sjögren's syndrome. This case supports the concept that a negative finding on labial biopsy does not rule out a diagnosis of Sjögren's syndrome.
In Vitro Effect of Nonsteroidal Antiinflammatory Drugs on Proteoglycanase and Collagenase Activity in Human Osteoarthritic Cartilage
Authors: Vignon Eric , Mathieu Pierre , Louisot Pierre , Richard Michel 
Published: February 2007

AbstractThe effects of several nonsteroidal antiinflammatory drugs, used at concentrations achievable in synovial fluid, on human osteoarthritic (OA) cartilage metalloprotease activity in vitro was studied. Acetaminophen and ketoprofen had no effect; sodium salicylate, indomethacin, and diclofenac slightly decreased proteoglycanase activity. Piroxicam and tenoxicam suppressed proteoglycanase activity by 48.2% and 68.3%, respectively, and suppressed collagenase activity by 19.1% and 36.8%, respectively. Use of these NSAIDs may help to decrease cartilage catabolism in patients with OA.
Biannual Radiographic Assessments of Hands and Feet in a Three‐Year Prospective Followup of Patients with Early Rheumatoid Arthritis
Authors: Heijde Désirée M. F. M. Van Der , Leeuwen Miek A. Van , Riel Piet L. C. M. Van , Koster Anja M. , Hof Martin A. Van't , Rijswijk Martin H. Van , Putte Levinus B. A. Van De 
Published: February 2007

AbstractIn a prospective followup study of 147 patients with rheumatoid arthritis of recent onset, we assessed the progression of radiographic evidence of joint damage on films of the patients' hands and feet obtained biannually. Patients were receiving first‐line and second‐line treatment. Ninety patients were followed up for 3 years, and 57 were followed up for only 2 years. Radiographic damage was determined by a modification of the method described by Sharp, and to ensure comparability of findings, we determined the percentage of damage per joint group (actual score divided by the maximum possible score). After 3 years, radiographic damage was present in 70% of the patients, all of whom could be identified after 1 year of study. Overall, 18—20% of the joints of the hands and feet were affected after 3 years, with relatively little abnormality per joint (˜8% of maximum possible score). During the entire followup, more foot joints than hand joints were affected. The rate of progression in the first year was significantly higher than in the second and third years of study, indicating a flattening of the curve of radiographic progression of joint damage.
Activation of Synovial Fluid T Lymphocytes by 60‐Kd Heat‐Shock Proteins in Patients with Inflammatory synovitis
Authors: Pope Richard M. , Lovis Rosa M. , Gupta Radhey S. 
Published: February 2007

AbstractObjective. Synovial fluid lymphocytes from patients with rheumatoid arthritis and with other forms of inflammatory synovitis demonstrate enhanced proliferative responses to Mycobacterium tuberculosis antigens, in particular, the 65‐kd heat‐shock protein. There is a high degree of homology between the human and the mycobacterial 60‐kd family of heat‐shock proteins. These studies were performed to determine if the enhanced response to the mycobacterial 65‐kd heat‐shock protein was due to cross‐reactivity of an immune response generated against the human homolog.Methods. These studies were performed by in vitro culture of isolated synovial fluid mononuclear cells with crude and purified antigens.Results. The synovial fluid lymphocytes of a majority of patients with rheumatoid arthritis recognized the mycobacterial 65‐kd heat‐shock protein, as evidenced by T cell proliferation. In contrast, only 18% of all samples tested responded to a highly purified recombinant human 60‐kd heat‐shock protein. With only one exception, proliferative responses to the mycobacterial antigen were stronger than those to the human homolog. The proliferative responses generated against mycobacterial 65‐kd heat‐shock proteins from different sources were highly correlated.Conclusion. The findings suggest that the enhanced proliferative response to the mycobacterial 65‐kd heat‐shock protein noted in most patients with rheumatoid arthritis and other forms of inflammatory synovitis is not due to cross‐reactivity of an immune response directed against the human heat‐shock protein.