bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation of 15 polymorphic microsatellite loci in Acacia hybrid (Acacia mangium×Acacia auriculiformis)
Authors: NG C. H. , KOH S. C. , LEE S. L. , NG K. K. S. , MARK A. , NORWATI M. , WICKNESWARI R. 
Published: March 2007

AbstractThis study reports the isolation of 15 microsatellites in Acacia hybrid (Acacia mangium×Acacia auriculiformis) based on the 5′ anchored polymerase chain reaction technique. Polymorphism of these loci was evaluated in a sample of 24 hybrid individuals. The level of polymorphism ranged from two to eight alleles with an observed heterozygosity ranging from 0.083 to 0.875. These loci were also characterized in both the parental species. The number of alleles ranged from two to six for both A. mangium and A. auriculiformis with the observed heterozygosity values ranging from 0.167 to 0.625 and 0.042 to 0.458 respectively. Five of these loci demonstrated Mendelian inheritance in a segregating F1 population; four presented a distorted ratio and the remaining six did not segregate in progenies as they were homozygous in both parents.
Isolation and characterization of polymorphic microsatellites from red coral grouper (Plectropomus maculatus)
Authors: ZHU Z. Y. , LO L. C. , LIN G. , XU Y. X. , YUE G. H. 
Published: March 2007

AbstractThe red coral grouper, Plectropomus maculatus, is a high value marine food fish species. We isolated the first set of 10 polymorphic microsatellites and examined their polymorphism in two related species: the Malabar grouper Epinephelus malabaricus and the brown‐marbled grouper Epinephelus fuscoguttatus. The average allele number of these 10 microsatellite DNA loci is 9.1/locus with a range of four to 17, whereas the expected heterozygosity ranged from 0.18 to 0.94, averaging 0.76. Five of the 10 markers amplified polymorphic and specific products in E. malabaricus, whereas only four were amplified in E. fuscoguttatus. These microsatellites could be applicable to captive breeding and to the studies on genetic diversity and population structure of grouper wild stocks.
Isolation and characterization of microsatellite loci in the highland papaya Vasconcellea × heilbornii V. Badillo (Caricaceae)
Authors: KYNDT TINA , HAEGEMAN ANNELIES , VAN GLABEKE SABINE , MAERTENS INGE , VAN DROOGENBROECK BART , ROLDÁN‐RUIZ ISABEL , GHEYSEN GODELIEVE 
Published: March 2007

AbstractNine polymorphic microsatellite loci were identified in the tropical plant Vasconcellea ×heilbornii and used to estimate allelic diversity in two populations of southern Ecuador. Allelic richness ranged from two to five alleles, observed heterozygosity ranged from 0.150 to 0.947 and expected heterozygosity ranged from 0.186 to 0.701. Most of these markers also amplified microsatellite loci in two other Vasconcellea species (Vasconcellea stipulata and Vasconcellea cundinamarcensis). Hence, these markers will be useful for population genetic analysis and the evaluation of genetic diversity and gene flow in these species.
pasos (parental allocation of singles in open systems): a computer program for individual parental allocation with missing parents
Authors: DUCHESNE PIERRE , CASTRIC TANGUY , BERNATCHEZ LOUIS 
Published: March 2007

Abstract pasos is a parental allocation program designed to identify collected parents based on individual multilocus genotypes while detecting missing parents when a proportion of them have not been collected. It makes use of restricted error tolerance in order to distinguish between a partially incorrect genotype from a false parent's genotype. pasos also introduces the technique of sequence allocation allowing the user to obtain estimates of the proportion of missing parents and of allocation correctness. The pasos interface is very similar to the one found in papa, its closed system counterpart (Duchesne et al. 2002). A help file thoroughly describes all technical terms such as error modelling, parameters and procedures. pasos can be downloaded free of charge from: http://www.bio.ulaval.ca/louisbernatchez/.
Locus effects and sources of error in noninvasive genotyping
Authors: BUCHAN JASON C. , ARCHIE ELIZABETH A. , VAN HORN RUSSELL C. , MOSS CYNTHIA J. , ALBERTS SUSAN C. 
Published: March 2007

AbstractIn spite of more than a decade of research on noninvasive genetic sampling, the low quality and quantity of DNA in noninvasive studies continue to plague researchers. Effects of locus size on error have been documented but are still poorly understood. Further, sources of error other than allelic dropout have been described but are often not well quantified. Here we analyse the effects of locus size on allelic dropout, amplification success and error rates in noninvasive genotyping studies of three species, and quantify error other than allelic dropout.
Ten new polymorphic microsatellite loci for North American river otters (Lontra canadensis) and their utility in related mustelids
Authors: BEHELER AMANDA S. , FIKE JENNIFER A. , DHARMARAJAN GUHA , RHODES OLIN E. , SERFASS THOMAS L. 
Published: March 2007

AbstractWe describe 10 novel North American river otter (Lontra canadensis) polymorphic microsatellite loci. Individuals from two river drainages in New York were sampled, and the number of alleles per locus ranged from two to 12 (Drainage 1) and two to 10 (Drainage 2). Observed heterozygosities ranged from 0.21 to 0.83 (Drainage 1) and 0.20 to 0.80 (Drainage 2). Preliminary screening revealed that loci amplified in five other mustelids [Martes pennanti (n = 6), Martes fiona (n = 8), Mustela frenata (n = 8), Mustela erminea (n = 8) and Mustela vison (n = 5)].
Polymorphic microsatellite DNA markers for a relictual angiosperm Cercidiphyllum japonicum Sieb. et Zucc. and their utility for Cercidiphyllum magnificum
Authors: ISAGI Y. , KUDO M. , OSUMI K. , SATO T. , SAKIO H. 
Published: March 2007

AbstractThe genus Cercidiphyllum is considered to be a primitive, relictual angiosperm, with two extant species occurring in limited areas of East Asia. We developed seven microsatellite markers for Cercidiphyllum japonicum, of which, five showed a clear, strong single band for each allele. The average number of alleles and observed heterozygosity were 26.2 and 0.82, respectively. Three pairs of polymerase chain reaction primers successfully amplified the DNA of the other species, Cercidiphyllum magnificum.
Additional microsatellites for Sparus aurata and cross‐species amplification within the Sparidae family
Authors: BROWN R. C. , TSALAVOUTA M. , TERZOGLOU V. , MAGOULAS A. , MCANDREW B. J. 
Published: March 2007

AbstractSix new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross‐species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species.
Microsatellite loci for behavioural studies of Eclectus parrot (Eclectus roratus: Aves)
Authors: ADCOCK G. J. , HEINSOHN R. , EBERT D. , AMINI N. , PEAKALL R. 
Published: March 2007

AbstractTen polymorphic microsatellite loci were isolated from the cooperatively breeding and sexually dichromatic Eclectus parrot (Eclectus roratus). Nine loci were in Hardy–Weinberg equilibrium and unlinked. One locus, Ero1, was presumed to be sex‐linked since females, the heterogametic sex, were all homozygous, whereas 72% of males were heterozygous. A DNA database search revealed that Ero8 is probably an independent isolation of the microsatellite locus AgGT83 of the parrot Amozona guildingii. With high heterozygosity (0.41–0.89) and number of alleles (two to 13), these loci should prove useful for investigating the mating system of these unusual Australasian parrots.
Simple sequence repeat markers for species in the Fusarium oxysporum complex
Authors: BOGALE M. , WINGFIELD B. D. , WINGFIELD M. J. , STEENKAMP E. T. 
Published: March 2007

AbstractWe describe nine simple sequence repeat (SSR) markers developed for studying Fusarium oxysporum. Allelic diversity at the nine loci ranged from 0.003 to 0.895, with a total of 71 alleles among 64 isolates. These markers will facilitate studies on relationships amongst isolates of F. oxysporum.
Microsatellite loci for population and behavioural studies of Horsfield's bronze‐cuckoo (Chalcites basalis: Aves)
Authors: ADCOCK G. J. , LANGMORE N. E. , MULDER R. A. , KILNER R. M. 
Published: March 2007

AbstractEight polymorphic microsatellite loci were identified for Horsfield's bronze‐cuckoo (Chalcites basalis). These include seven newly isolated loci from cuckoo genomic libraries enriched for GA and GAAA repeat‐containing clones. These loci have a mean expected heterozygosity of 0.71, a mean number of alleles of 13.8 and a combined exclusion probability (one parent known) of 0.9999. Two loci (Cba01 and Cba07) showed a significant deficiency of heterozygotes and may therefore have null alleles, although this effect could be the result of nonrandom population sampling.
Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross‐species amplification in closely related taxa
Authors: SCHNELL R. J. , OLANO C. T. , QUINTANILLA W. E. , MEEROW A. W. 
Published: March 2007

AbstractWe report here on the development and characterization of 15 microsatellite loci isolated from Mangifera indica L. These markers were evaluated using 59 Florida cultivars and four related species from the USDA germplasm collection for mango. Two loci were monomorphic and 13 polymorphic, with two to seven alleles per locus. Four loci departed significantly from Hardy–Weinberg equilibrium and have significant heterozygote deficiency. Nine loci exhibited significant linkage disequilibrium. Cross‐species amplification was successful in four related species. These loci are being used to investigate patterns of genetic variation within M. indica and between closely related species.
Microsatellite markers for assessing DNA polymorphism of Emericella nidulans in nature
Authors: HOSID E. , GRISHKAN I. , FRENKEL Z. , WASSER S. P. , NEVO E. , KOROL A. 
Published: March 2007

AbstractBased on genome sequence of Emericella nidulans, primers for 25 microsatellites were designed, of which, seven were chosen as polymorphic markers (five to 12 alleles). Simple sequence repeat (SSR) polymorphism was tested in 44 isolates from extremely arid soils of the southern Negev desert, Israel and some related species of Aspergillus. The SSR marker set appears suitable for population studies in diverse ecological conditions.
Characterization of microsatellite loci in the brown treecreeper (Climacteris picumnus) and cross‐species amplification in the white‐throated treecreeper (Cormobates leucophaeus)
Authors: DOERR ERIK D. 
Published: March 2007

AbstractEight polymorphic microsatellite markers were developed for the brown treecreeper, Climacteris picumnus. The number of alleles ranged from three to 25 per locus with observed heterozygosities between 0.05 and 0.76. Seven of the eight primer pairs also amplified polymorphic microsatellite loci in the white‐throated treecreeper (Cormobates leucophaeus). These markers are likely to be useful for population genetic and parentage studies in any of the Australasian treecreepers (Climacteridae) and are the first genetic markers developed for any member of this passerine family.
Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward
Authors: PANNACCIULLI FEDERICA G. , PIYAPATTANAKORN SANIT , BISHOP JOHN D. D. , HAWKINS STEPHEN J. , MACLEAN NORMAN 
Published: March 2007

AbstractThis study reports the isolation and characterization of seven highly polymorphic microsatellite loci in Chthamalus montagui (Crustacea, Cirripedia). The loci were isolated from a library constructed from genomic DNA enriched for CA repeats. The markers yielded three to 43 alleles per locus (mean 16.7) in samples averaging 49 individuals. Observed heterozygosity ranged from 0.08 to 0.58 (mean 0.39). These microsatellite loci will be valuable tools for population genetic studies of this species.
Polymorphic microsatellite DNA markers for the rhinoceros auklet (Cerorhinca monocerata)
Authors: HASEGAWA O. , ISHIBASHI Y. , ABE S. 
Published: March 2007

AbstractEight polymorphic microsatellite loci were isolated from the partial genomic library of the rhinoceros auklet Cerorhinca monocerata. The heterozygosities observed at the isolated loci using eight primer sets in 46 nestlings from one breeding colony in Japan ranged from 0.311 to 0.773, and all but one locus did not deviate from the Hardy–Weinberg expectations. Cross‐species amplification in the tufted puffin Fratercula cirrhata was successfully performed using six of the eight primer sets, indicating their applicability to this species.
gerud 2.0: a computer program for the reconstruction of parental genotypes from half‐sib progeny arrays with known or unknown parents
Authors: JONES ADAM G. 
Published: March 2007

AbstractLarge groups of related progeny, as can be collected from discrete egg masses or fruits, present excellent opportunities for parentage analysis by allowing the reconstruction of parental genotypes. Current techniques of parental genotypic reconstruction require the knowledge of at least one parental genotype. Here, I present a new computer program that reconstructs parental genotypes when no parents are known, provided that the progeny array contains only full and half siblings. The prospects for successfully reconstructing parental genotypes for such progeny arrays with unknown parents are nearly as good as those for the case when one parent is known with certainty.
colonise: a computer program to study colonization processes in metapopulations
Authors: FOLL MATTHIEU , GAGGIOTTI OSCAR E. 
Published: March 2007

Abstract colonise is a user‐friendly Microsoft® Windows software for the study of colonization events in natural populations. It determines the composition of the newly colonized population and makes inferences about the factors that influenced individuals to establish a new population. colonise uses a hierarchical Bayesian model to combine multilocus genotype data with demographic and environmental data, and a reversible jump Markov chain Monte Carlo (MCMC) algorithm to perform posterior density estimations. The software is written in C and C + + for the graphical user interface and is available at http://www2.ujf‐grenoble.fr/leca/presentation.html. The software integrates a tool to draw posterior density functions (histogram, running mean, traces, etc.) and to estimate parameters from them (mean, mode, variance, HPDI, etc.).
A technique for the rapid extraction of microalgal DNA from single live and preserved cells
Authors: RICHLEN M. L. , BARBER P. H. 
Published: March 2007

AbstractMolecular methods are increasingly being used in the study of harmful microalgae; however, DNA extraction techniques have imposed limitations on the species and questions studied, with research primarily restricted to cultured specimens. Here we describe a simple method that merges two existing techniques for DNA extraction from live and preserved single dinoflagellate cells. DNA was successfully isolated from live single cells of Gambierdiscus toxicus Adachi et Fukuyo, 1979 and cells preserved using formalin/methanol fixation. This method supplements existing techniques and expands the scope of genetics studies conducted on dinoflagellates to include routine molecular analysis of single cells isolated from field samples.
Isolation and characterization of microsatellite markers in Ijima's leaf warbler, Phylloscopus ijimae (Aves: Sylviidae)
Authors: SAITO D. S. , SAITOH T. , NISHIUMI I. 
Published: March 2007

AbstractMicrosatellite markers were isolated from Ijima's leaf warbler, Phylloscopus ijimae. From an enriched genomic library and using the fast isolation by AFLP of sequences containing repeats (FIASCO) method, 10 polymorphic loci were obtained. Four to 12 alleles were identified in an analysis of 23 Ijima's leaf warblers, with the degree of heterozygosity ranging from 0.35 to 0.87. The markers were tested in four species of Phylloscopus and two other non‐Phylloscopus species of Sylviidae. Some loci were successfully amplified and showed polymorphism in Phylloscopus species, whereas amplification in the non‐Phylloscopus species was less successful.
Comprehensive DNA barcode coverage of North American birds
Authors: KERR KEVIN C. R. , STOECKLE MARK Y. , DOVE CARLA J. , WEIGT LEE A. , FRANCIS CHARLES M. , HEBERT PAUL D. N. 
Published: March 2007

AbstractDNA barcoding seeks to assemble a standardized reference library for DNA‐based identification of eukaryotic species. The utility and limitations of this approach need to be tested on well‐characterized taxonomic assemblages. Here we provide a comprehensive DNA barcode analysis for North American birds including 643 species representing 93% of the breeding and pelagic avifauna of the USA and Canada. Most (94%) species possess distinct barcode clusters, with average neighbour‐joining bootstrap support of 98%. In the remaining 6%, barcode clusters correspond to small sets of closely related species, most of which hybridize regularly. Fifteen (2%) currently recognized species are comprised of two distinct barcode clusters, many of which may represent cryptic species. Intraspecific variation is weakly related to census population size and species age. This study confirms that DNA barcoding can be effectively applied across the geographical and taxonomic expanse of North American birds. The consistent finding of constrained intraspecific mitochondrial variation in this large assemblage of species supports the emerging view that selective sweeps limit mitochondrial diversity.
Isolation and characterization of short tandem repeats in an invasive swimbladder nematode, parasitic in Atlantic freshwater eels, Anguillicola crassus
Authors: WIELGOSS SÉBASTIEN , SANETRA MATTHIAS , MEYER AXEL , WIRTH THIERRY 
Published: March 2007

AbstractWe describe the isolation and characterization of seven polymorphic short tandem repeats (STR) for the eel parasite Anguillicola crassus. This invasive swimbladder nematode endemic in East Asia was recently introduced into Europe. The number of alleles for each STR ranged from 13 to 39 per locus with observed heterozygosities between 0.49 and 0.98. The Taiwanese population displayed higher genetic diversity compared to the Irish sample, an observation consistent with the Asian biogeographical origin of the nematode. Availability of the reported STR will facilitate the investigation of the population genetic structure with regard to multiple invasions.
Performance Measure Congruity and the Balanced Scorecard
Authors: BUDDE JÖRG 
Published: March 2007

ABSTRACTThis paper studies the incentive effects of a balanced scorecard within a multitask agency framework under both formal and relational contracts. First, the main characteristics of the balanced scorecard are analyzed with respect to performance measure congruity. It is shown that under complete verifiability, a properly designed balanced scorecard is capable of perfectly aligning the interests of owners and employees by means of an explicit contract. I then investigate whether subjective performance evaluation is beneficial when not all the scorecard measures are contractible. It emerges that congruity of the contractible scorecard measures constrains a purely implicit incentive contract, but the first‐best solution may still be obtained through a combination of formal and relational contracts. Furthermore, a purely explicit contract in most cases can be improved by incorporating subjective rewards.
Directional Preferences, Information Processing, and Investors' Forecasts of Earnings
Authors: HALES JEFFREY 
Published: March 2007

ABSTRACTThis paper investigates the effects of preferences on judgments in an investing context, where investors should be motivated to interpret information objectively, yet have clear preferences with respect to what the information they are evaluating conveys (i.e., a gain or a loss on their investment). The results of the experiment are consistent with theories of motivated reasoning that predict when and in what manner directional preferences affect how information is processed. Specifically, investors are motivated to agree unthinkingly with information that suggests they might make money on their investment, but disagree with information that suggests they might lose money. In disagreeing, long investors expect earnings to be relatively high and short investors expect earnings to be relatively low. These results have implications not only for understanding investor behavior, but also for understanding the biased behavior of market participants who face conflicts of interest, such as analysts, managers, and auditors, by providing direct evidence that such behavior can arise for purely psychological reasons.
Beta‐Subunits Are Important Modulators of the Acute Response to Alcohol in Human BK Channels
Authors: Feinberg‐Zadek Paula L. , Treistman Steven N. 
Published: March 2007

Background: The BK channel (a Ca2+‐activated potassium ion channel encoded by the slo gene) has been defined as a target of alcohol action in a number of preparations, possibly serving as primary mediator of intoxication in the Caenorhabditis elegans model system. However, we know little of the actions of alcohol on human BK, nor the consequences of BK subunit composition on alcohol action.Methods: Here, we use human embryonic kidney (HEK) cells to express various subunit combinations (hslo α+β1 or β4) of human BK, and examine the acute actions of alcohol on this channel using single channel recording techniques.Results: The human channel is potentiated by alcohol, although the presence of the β1, and to a lesser extent, β4‐subunit, significantly reduced acute ethanol potentiation. Potentiation increased with concentration up to an asymptote, at which point potentiation decreased. The concentration of the asymptote differed according to subunit composition. The mechanism of potentiation was also subunit‐dependent, with 25 mM ethanol affecting the mean open time of hSlo+β4 channels, whereas channel open time was unaffected by the presence of β1. The possibility that the known effect of the β‐subunit on calcium sensitivity accounts for its modulation of acute alcohol action is discussed.Conclusion: Our data reinforce the idea that, as in other systems, BK may play a major role in alcohol's actions in humans, and highlight the potential role of channel subunit composition in the response to alcohol.