bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excavating important nodes in complex networks based on the heat conduction model
Authors: Hu Haifeng , Zheng Junhui , Hu Wentao , Wang Feifei , Wang Guan , Zhao Jiangwei , Wang Liugen 
Published: April 2024

AbstractAnalyzing the important nodes of complex systems by complex network theory can effectively solve the scientific bottlenecks in various aspects of these systems, and how to excavate important nodes has become a hot topic in complex network research. This paper proposes an algorithm for excavating important nodes based on the heat conduction model (HCM), which measures the importance of nodes by their output capacity. The number and importance of a node’s neighbors are first used to determine its own capacity, its output capacity is then calculated based on the HCM while considering the network density, distance between nodes, and degree density of other nodes. The importance of the node is finally measured by the magnitude of the output capacity. The similarity experiments of node importance, sorting and comparison experiments of important nodes, and capability experiments of multi-node infection are conducted in nine real networks using the Susceptible-Infected-Removed model as the evaluation criteria. Further, capability experiments of multi-node infection are conducted using the Independent cascade model. The effectiveness of the HCM is demonstrated through a comparison with eight other algorithms for excavating important nodes.
Lengthened circadian rhythms in mice with self-controlled ambient light intensity
Authors: Ogasawara Jun , Matsumoto Nobuyoshi , Takeuchi Yuki , Yamashiro Kotaro , Yasui Masato , Ikegaya Yuji 
Published: April 2024

AbstractLaboratory animals are typically maintained under 12-h light and 12-h dark (12:12 LD) conditions with a daytime light intensity of ~ 200 lx. In this study, we designed an apparatus that allowed mice to self-select the room light intensity by nose poking. We measured the behavioral rhythms of the mice under this self-controlled light regimen. The mice quickly learned the relationship between their nose pokes and the resulting changes in the light intensity. Under these conditions, the mice exhibited free-running circadian behavior with a period of 24.5 ± 0.4 h. This circadian period was ~ 1 h longer than that of the same strain of mice when they were kept in constant darkness (DD) after 12:12 LD entrainment, and the lengthened period lasted for at least 30 days. The rhythm of the light intensity controlled by the mice also exhibited a similar period, but the phase of the illuminance rhythm preceded the phase of the locomotor activity rhythm. Mice that did not have access to the light controller were also entrained to the illuminance cycle produced by the mice that did have access to the light controller, but with a slightly delayed phase. The rhythm was likely controlled by the canonical circadian clock because mice with tau mutations in the circadian clock gene CSNK1E exhibited short periods of circadian rhythm under the same conditions. These results indicate that the free-running period of mice in the wild may differ from what they exhibit if they are attuned by forced light cycles in laboratories because mice in their natural habitats can self-control their exposure to ambient light, similar to our experimental conditions.
B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer
Authors: Hui Yuan-Jian , Yu Ting-Ting , Li Liu-Gen , Peng Xing-Chun , Di Mao-Jun , Liu Hui , Gu Wen-Long , Li Tong-Fei , Zhao Kai-Liang , Wang Wei-Xing 
Published: April 2024

AbstractB-Myb has received considerable attention for its critical tumorigenic function of supporting DNA repair. However, its modulatory effects on chemotherapy and immunotherapy have rarely been reported in colorectal cancer. Bortezomib (BTZ) is a novel compound with chemotherapeutic and immunotherapeutic effects, but it fails to work in colorectal cancer with high B-Myb expression. The present study was designed to investigate whether B-Myb deletion in colorectal cancer could potentiate the immune efficacy of BTZ against colorectal cancer and to clarify the underlying mechanism. Stable B-Myb knockdown was induced in colorectal cancer cells, which increased apoptosis of the cancer cells relative to the control group in vitro and in vivo. We found that BTZ exhibited more favourable efficacy in B-Myb–defective colorectal cancer cells and tumor-bearing mice. BTZ treatment led to differential expression of genes enriched in the p53 signaling pathway promoted more powerful downstream DNA damage, and arrested cell cycle in B-Myb–defective colorectal cancer. In contrast, recovery of B-Myb in B-Myb–defective colorectal cancer cells abated BTZ-related DNA damage, cell cycle arrest, and anticancer efficacy. Moreover, BTZ promoted DNA damage–associated enhancement of immunogenicity, as indicated by potentiated expression of HMGB1 and HSP90 in B-Myb–defective cells, thereby driving M1 polarization of macrophages. Collectively, B-Myb deletion in colorectal cancer facilitates the immunogenic death of cancer cells, thereby further promoting the immune efficacy of BTZ by amplifying DNA damage. The present work provides an effective molecular target for colorectal cancer immunotherapy with BTZ.
Exploring secondary optical transitions: a study utilizing the DITM method, and enhanced photocatalytic properties in Ni-doped CuSe
Authors: Ghobadi Nader , Zamani Meymian Mohammad-Reza , Fallah Milad 
Published: April 2024

AbstractThis study explores the simultaneous presence of two metal ions of Nickel (Ni) and Copper (Cu) on the formation of a metal selenide (Ni-doped CuSe) in an alkaline environment. The impact of Ni ions on creating the second optical transitions is investigated. Different concentrations amounts of Ni ions (0.01, 0.02, and 0.03 mol) are utilized to produce Ni-doped CuSe semiconductor thin films through a chemical solution deposition method with deposition times varying from 3 to 6 h. Absorbance spectra are employed to determine the band-gap, while Field Emission Scanning Electron Microscopy is utilized for morphological analysis. Structural and elemental analyses are conducted using X-ray Diffraction and Energy Dispersive X-ray Spectroscopy techniques. Additionally, a relatively innovative approach for determining the optical transitions, termed the Derivation Ineffective Thickness Method (DITM), is employed. DITM eliminates the need for thin film thickness and assumptions about the type of transition (direct or indirect) for band-gap calculation. Moreover, a comparison is made between the band-gap obtained from the Tauc model and the transitions obtained by DITM method. Furthermore, it is demonstrated that the optical transitions exhibit two distinct band-gaps associated with nickel selenide (NiSe) as second transition and copper selenide (CuSe) as fundamental transition. The presence of Ni is also found to enhance crystal quality. The study also briefly explores the improved photocatalytic properties of CuSe in the presence of Ni.
Estrogenic endocrine disruptor exposure directly impacts erectile function
Authors: Cripps Samuel M. , Marshall Sarah A. , Mattiske Deidre M. , Ingham Rachel Y. , Pask Andrew J. 
Published: April 2024

AbstractErectile dysfunction (ED) is an extremely prevalent condition which significantly impacts quality of life. The rapid increase of ED in recent decades suggests the existence of unidentified environmental risk factors contributing to this condition. Endocrine Disrupting Chemicals (EDCs) are one likely candidate, given that development and function of the erectile tissues are hormonally dependent. We use the estrogenic-EDC diethylstilbestrol (DES) to model how widespread estrogenic-EDC exposure may impact erectile function in humans. Here we show that male mice chronically exposed to DES exhibit abnormal contractility of the erectile tissue, indicative of ED. The treatment did not affect systemic testosterone production yet significantly increased estrogen receptor α (Esr1) expression in the primary erectile tissue, suggesting EDCs directly impact erectile function. In response, we isolated the erectile tissue from mice and briefly incubated them with the estrogenic-EDCs DES or genistein (a phytoestrogen). These acute-direct exposures similarly caused a significant reduction in erectile tissue contractility, again indicative of ED. Overall, these findings demonstrate a direct link between estrogenic EDCs and erectile dysfunction and show that both chronic and acute estrogenic exposures are likely risk factors for this condition.
FAM120A deficiency improves resistance to cisplatin in gastric cancer by promoting ferroptosis
Authors: Niu Liangbo , Li Yi , Huang Guixiang , Huang Wei , Fu Jing , Feng Lu 
Published: April 2024

AbstractThe occurrence of chemoresistance is an inescapable obstacle affecting the clinical efficacy of cisplatin in gastric cancer (GC). Exploring the regulatory mechanism of cisplatin resistance will help to provide potential effective targets for improving the prognosis of gastric cancer patients. Here, we find that FAM120A is upregulated in GC tissues and higher in cisplatin-resistant GC tissues, and its high expression is positively correlated with the poor outcome of GC patients. Functional studies indicate that FAM120A confers chemoresistance to GC cells by inhibiting ferroptosis. Mechanically, METTL3-induced m6A modification and YTHDC1-induced stability of FAM120A mRNA enhance FAM120A expression. FAM120A inhibits ferroptosis by binding SLC7A11 mRNA and enhancing its stability. FAM120A deficiency enhances cisplatin sensitivity by promoting ferroptosis in vivo. These results reveal the function of FAM120A in chemotherapy tolerance and targeting FAM120A is an effective strategy to alleviate cisplatin resistance in GC.
Cut-insert-stitch editing reaction (CIStER) sequence for surgical chemical glycan editing
Authors: Sen Sumit , Kundu Suman , Pasari Sandip , Hotha Srinivas 
Published: April 2024

AbstractPost-synthetic surgical editing enables synthesizing diverse molecules from a common scaffold. Editing carbohydrates by inserting a foreign glycan is still a far-reaching goal for synthetic chemists. In this study, a one-pot-three-step chemical approach was employed to edit glycoconjugates. It is comprised of three steps: the first is a ‘cut’ step, cleaving one of the interglycosidic bonds and producing an intermediate that could be intercepted with 4-mercaptotoluene; second step activates the thiotolyl glycoside in the presence of an aglycon containing an orthogonally activatable ethynylcycloxyl carbonate moiety; and the third step involves ‘stitching’ by activating the carbonate donor. The cut-insert stitch-editing reaction (CIStER) is demonstrated by inserting branched and linear arabinans reminiscent of M. tuberculosis cell wall from the same designer trimannoside. Glycosylating an activated hydroxyacid (serinyl, steroidal, and lipid) after cutting the interglycosidic bond and stitching in the presence of base extendes the CIStER approach to the synthesis of glycohybrids.
Exploring urban housing disadvantages and economic struggles in Seoul, South Korea
Authors: Lee Yookyung , Han Seungwoo 
Published: April 2024

AbstractThe present study investigates urban poverty in Seoul, South Korea, amid the COVID-19 pandemic, focusing on housing and economic challenges. Employing principal component analysis, clustering algorithms, and visualization techniques, it analyzes archived data to uncover disparities in housing conditions and economic well-being across Seoul. The research reveals significant socio-economic divisions, with over 75% of the city’s areas marked by vulnerability, indicating widespread poverty or the concentration of economically disadvantaged populations. This highlights the pervasive nature of poverty and the precarious situation of the urban poor, who are at risk due to fragile living conditions. The findings advocate for inclusive urban development strategies that prioritize the needs of marginalized groups, suggesting a shift from focusing solely on economic growth to ensuring equitable welfare for all residents.
Signal improved ultra-fast light-sheet microscope for large tissue imaging
Authors: Prince Md Nasful Huda , Garcia Benjamin , Henn Cory , Yi Yating , Susaki Etsuo A. , Watakabe Yuki , Nemoto Tomomi , Lidke Keith A. , Zhao Hu , Remiro Irene Salinas , Liu Sheng , Chakraborty Tonmoy 
Published: April 2024

AbstractAxially swept light-sheet microscope in conjunction with tissue clearing enables three-dimensional morphological investigation of millimeter-scaled tissues at isotropic sub-micron resolution. However, these microscopes suffer from low detection signal and slow imaging speed. Here we report a simple and efficient imaging platform that employs precise control of two fixed distant light-sheet foci for axial sweeping. This enables full field of view imaging at 40 frames per second, a four-fold improvement over the current state-of-the-art. In addition, in a particular frame rate, our method doubles the signal compared to the existing techniques. To augment the overall imaging performance, we also developed a deep learning based tissue information classifier that enables faster determination of tissue boundary. We demonstrated the performance of our imaging platform on various cleared tissue samples and delineated its robustness over a wide range of clearing protocols.
Large language models could change the future of behavioral healthcare: a proposal for responsible development and evaluation
Authors: Stade Elizabeth C. , Stirman Shannon Wiltsey , Ungar Lyle H. , Boland Cody L. , Schwartz H. Andrew , Yaden David B. , Sedoc João , DeRubeis Robert J. , Willer Robb , Eichstaedt Johannes C. 
Published: April 2024

AbstractLarge language models (LLMs) such as Open AI’s GPT-4 (which power ChatGPT) and Google’s Gemini, built on artificial intelligence, hold immense potential to support, augment, or even eventually automate psychotherapy. Enthusiasm about such applications is mounting in the field as well as industry. These developments promise to address insufficient mental healthcare system capacity and scale individual access to personalized treatments. However, clinical psychology is an uncommonly high stakes application domain for AI systems, as responsible and evidence-based therapy requires nuanced expertise. This paper provides a roadmap for the ambitious yet responsible application of clinical LLMs in psychotherapy. First, a technical overview of clinical LLMs is presented. Second, the stages of integration of LLMs into psychotherapy are discussed while highlighting parallels to the development of autonomous vehicle technology. Third, potential applications of LLMs in clinical care, training, and research are discussed, highlighting areas of risk given the complex nature of psychotherapy. Fourth, recommendations for the responsible development and evaluation of clinical LLMs are provided, which include centering clinical science, involving robust interdisciplinary collaboration, and attending to issues like assessment, risk detection, transparency, and bias. Lastly, a vision is outlined for how LLMs might enable a new generation of studies of evidence-based interventions at scale, and how these studies may challenge assumptions about psychotherapy.
Experiencing nature leads to healthier food choices
Authors: Langlois Maria , Chandon Pierre 
Published: April 2024

AbstractExperiencing nature has been linked to a host of benefits for health and well-being. Here, we examine if exposure to nature influences the food choices that may contribute to nature’s benefits. Five between-subject experiments (n = 39, n = 698, n = 885, n = 1191, and n = 913) show that individuals exposed to the natural environment choose healthier foods when compared to those exposed to urban environments or a control condition. Nature’s effects are observed for various foods and beverages, across samples from three countries, and in varied contexts, such as taking a walk in a park (vs. a city street) and looking at photos of nature (vs. urban or control) scenes. These findings provide insights into the relationship between proximity to nature and health.
Molecular mechanism of Oxr1p mediated disassembly of yeast V-ATPase
Authors: Khan Md. Murad , Wilkens Stephan 
Published: April 2024

AbstractThe eukaryotic vacuolar H+-ATPase (V-ATPase) is regulated by reversible disassembly into autoinhibited V1-ATPase and Vo proton channel subcomplexes. We recently reported that the TLDc protein Oxr1p induces V-ATPase disassembly in vitro. Whether and how Oxr1p is involved in enzyme disassembly in vivo, however, is not known. Here, using yeast genetics and fluorescence microscopy, we show that Oxr1p is essential for efficient V-ATPase disassembly in the cell. Supporting biochemical and biophysical in vitro experiments show that whereas Oxr1p-driven holoenzyme disassembly can occur in the absence of nucleotides, the presence of ATP greatly accelerates the process. ATP hydrolysis is needed, however, for subsequent release of Oxr1p so that the free V1 can adopt the autoinhibited conformation. Overall, our study unravels the molecular mechanism of Oxr1p-induced disassembly that occurs in vivo as part of the canonical V-ATPase regulation by reversible disassembly.
ImmunoPET imaging of Trop2 in patients with solid tumours
Authors: Huang Wei , Zhang You , Cao Min , Wu Yanfei , Jiao Feng , Chu Zhaohui , Zhou Xinyuan , Li Lianghua , Xu Dongsheng , Pan Xinbing , Guan Yihui , Huang Gang , Liu Jianjun , Xie Fang , Wei Weijun 
Published: April 2024

AbstractAccurately predicting and selecting patients who can benefit from targeted or immunotherapy is crucial for precision therapy. Trophoblast cell surface antigen 2 (Trop2) has been extensively investigated as a pan-cancer biomarker expressed in various tumours and plays a crucial role in tumorigenesis through multiple signalling pathways. Our laboratory successfully developed two 68Ga-labelled nanobody tracers that can rapidly and specifically target Trop2. Of the two tracers, [68Ga]Ga-NOTA-T4, demonstrated excellent pharmacokinetics in preclinical mouse models and a beagle dog. Moreover, [68Ga]Ga-NOTA-T4 immuno-positron emission tomography (immunoPET) allowed noninvasive visualisation of Trop2 heterogeneous and differential expression in preclinical solid tumour models and ten patients with solid tumours. [68Ga]Ga-NOTA-T4 immunoPET could facilitate clinical decision-making through patient stratification and response monitoring during Trop2-targeted therapies.
Acute-Onset Acquired Stuttering Secondary to Concussion: A Case Report
Authors: Wagner Kelsey Jo , Hux Karen 
Published: April 2024

Purpose:
Acquired stuttering in young adults can present diagnostic and treatment challenges. This single case report documents the diagnosis of acquired, postconcussive, acute-onset stuttering in an 18-year-old man, describes key elements of a person-centered treatment program conducted via a telerehabilitation platform, and reports documented changes in speech production following intervention.

Method:
Person-centered care principles, disfluency education, strategies for managing stuttering episodes, and a home program were treatment components. Using the Overall Assessment of the Speaker's Experience of Stuttering (OASES) and speech sample analyses, documentation of initial status and changes over time came from initial, 3-month, 6-month, and follow-up evaluations.

Results:
Initial OASES scores were consistent with moderate–severe impact for General Information, moderate impact to Daily Communication, and mild–moderate impact with respect to Speaker's Reactions, Quality of Life, and Overall Assessment. The initial speech sample analysis revealed severely disrupted speech production with disfluencies occurring on 60.47% of syllables. In contrast, the 6-month assessment revealed mild or mild–moderate OASES impact scores and disfluencies on 1.78% of syllables. Performance during a follow-up evaluation held 3 months following treatment termination confirmed maintenance of fluent speech production both when reading and engaging in conversation.

Conclusion:
Clear differentiation between neurogenic and functional contributions to acute-onset, acquired stuttering is not always possible. In some cases, a combination of precipitating factors may be evident. Regardless, presenting traditional fluency management strategies within a framework of person-centered care can be an effective treatment approach.

Supplemental Material:

https://doi.org/10.23641/asha.25463968
A compact 60‐GHz on‐chip bandpass filter in GaAs technology
Authors: Xu Kai‐Da , Xi Shangjing , Liu Hairui , Chen Shichang 
Published: April 2024

AbstractA compact 60‐GHz on‐chip bandpass filter (BPF) is presented using gallium arsenide (GaAs) technology. To demonstrate the working mechanism of the proposed BPF, an inductor‐capacitor (LC) equivalent circuit model is conceived and analysed for further investigation of the transmission poles (TPs) and zeros. Finally, a prototype of the BPF is fabricated and tested to validate the proposed idea, whose simulated and measured results are in good agreement. The measurements show that it has a centre frequency of 60.2 GHz with a bandwidth of 14%, and the minimum insertion loss (IL) within the passband is 2.57 dB. The chip size, excluding the feedings, is about 0.265 mm × 0.184 mm.
Multi‐objective hierarchical optimisation design and experimental verification of an alterable‐magnetic‐circuit variable‐flux memory machine
Authors: Zhang Shukuan , Wang Fachen , Zhu Jingwei , Zheng Ping , Liu Guangwei 
Published: April 2024

AbstractRare‐earth permanent magnet synchronous machines face challenges in manipulating their magnetic fields, which hinders the ability to extend the operation speed range. Moreover, this inflexibility leads to reduced efficiency in high‐speed scenarios when the machine is under flux‐weakening control and increases the risk of the magnets becoming demagnetised. The authors propose an alterable‐magnetic‐circuit variable‐flux memory machine (AMC‐VFMM) and a multi‐objective hierarchical optimisation method is conducted to optimise the machine. Firstly, the topology and alterable‐magnetic‐circuit principle of the proposed AMC‐VFMM are introduced. Then, optimisation objectives including torque production capability, flux regulation capability, and resisting unintentional demagnetisation capability are defined, and the hierarchical optimisation approach is established by stratifying the optimisation objectives and variables through the sensitivity analysis. Finite element analysis indicates that electromagnetic performances of the optimised design scheme are significantly enhanced. The bench test of the prototype demonstrates the superiority of the proposed AMC‐VFMM and validates the effectiveness of the optimisation design method.