bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presence and physical state of HPV DNA in prostate and urinary‐tract tissues
Authors: Rotola A. , Monini P. , di Luca D. , Savioli A. , Simone R. , Secchiero P. , Reggiani A. , Cassai E. 
Published: February 2007

AbstractNeoplastic and non‐neoplastic tissues from the urinary tract and the prostate were analyzed for the presence of human papillomavirus (HPV) DNA. The analysis was performed by PCR using primers specific for HPV 6/11 and 16. HPV DNA was present in bladder, ureter, kidney and prostate, with percentages ranging between 46% and 87%. Benign and oncogenic HPV types were detected with similar frequencies both in non‐neoplastic and in neoplastic biopsies, and HPV 16 was not preferentially associated with malignant lesions. In all instances, small amounts of HPV DNA were present in the tissues, suggesting the absence of productive infection. Analysis of the physical state of HPV DNA performed by 2‐dimensional gel electrophoresis and Southern blot hybridization revealed that HPV 16 DNA harbored in the urinary tract can be integrated also in non‐neoplastic tissues. The results indicate that HPV 16 does not seem to be associated with urinary‐tract and prostate oncogenesis, but that these tissues may represent an important reservoir for the transmission of HPV types normally infecting the genital tract. © 1992 Wiley‐Liss, Inc.
Immunogenic tumor variants induced by drug treatment of the L5178Y lymphoma: Search for serologically defined antigens at the clonal level
Authors: Grohmann U. , Puccetti P. , Romani L. , Binaglia L. , Bianchi R. , Belladonna M. L. , Ullrich S. J. , Appella E. , Fioretti M. C. 
Published: February 2007

AbstractHighly immunogenic tumor variants are generated by in vitro or in vivo treatment of the murine L5178Y lymphoma line with triazene derivatives. Most of these variants express new transplantation‐ and antibody‐defined antigens that previous studies have shown to be closely related. One such 80‐kDa protein on the surface of clone‐D cells was found to be related to xenotropic MuLV gp70 molecules. To investigate the possible relevance of clone‐D data to general properties of immunogenic variants in this tumor model system, polyclonal syngeneic antisera raised to a panel of immunogenic clones (including clone D) of the drug‐treated L5178Y lymphoma line were employed in the immunoprecipitation of cell‐surface and intrinsically labeled variant cells. In all clones, 1‐ and 2‐dimensional electrophoretic analysis of the immunoprecipitates detected an antigen of approximately 80 kDa, and 35S‐labeled 80‐kDa molecules could be cross‐precipitated from all clones by the panel of clone‐ specific antisera. In addition, 45‐ and 30‐kDa components were also found in metabolically labeled variant cells. While the surface 80‐kDa component was reactive with anti‐xenotropic gp70 antibodies, the 30‐kDa molecule was removed by anti‐gag p30 antibody in sequential immunoprecipitation experiments. These data suggest that expression of aberrant, retrovirus‐related proteins is a common finding in immunogenic cells of the drug‐treated L5178Y lymphoma line. © 1992 Wiley‐Liss, Inc.
Enhanced epidermal growth factor receptor synthesis in human squamous carcinoma cells exposed to low levels of oxygen
Authors: Laderoute Keith R. , Grant Tandora D. , Murphy Brian J. , Sutherland R. M. 
Published: February 2007

AbstractExposure of human A431 squamous carcinoma cells to levels of hypoxia found in some solid tumors causes 2‐fold increases in epidermal growth‐factor receptor (EGF‐R) mRNA levels and rate of receptor protein synthesis compared with aerobic cells. Similar results are shown for receptor message from other squamous carcinoma cells, human keratinocytes, and human W138 fibroblasts. Less basal tyrosine phosphorylation of the receptor occurs in hypoxic compared with aerobic A431 cells. Scatchard analysis also shows that reoxygenated A431 cells display enhanced surface expression of the EGF‐R compared with aerobic control cells. Possible mechanisms and implications for tumor therapy are discussed. © 1992 Wiley‐Liss, Inc.
Distribution and photosensitizing efficiency of porphyrins induced by application of exogenous 5‐aminolevulinic acid in mice bearing mammary carcinoma
Authors: Peng Q. , Moan J. , Warloe T. , Nesland J. M. , Rimington C. 
Published: February 2007

AbstractBy means of a chemical extraction procedure and confocal laser scanning microscopy, we investigated the kinetic patterns of uptake and biolocalization of 5‐aminolevulinic acid (ALA)‐induced porphyrins in s.c. transplanted tumors, adjacent normal skin and muscle, and liver of mice bearing mammary carcinoma, after i.p. injection of 250 mg/kg ALA or topical application of ALA (20% in an oil‐in‐water emulsion). Furthermore, we evaluated the tumor responses after either i.p. injection or topical application of 5‐ALA followed by laser irradiation (632 nm, 150 mW/cm2, 25 min) by measuring the treated tumor regression/regrowth time and by light and electron microscopy. Strong fluorescence of ALA‐induced porphyrins was detected in the tumor, skin and liver tissues, while little fluorescence was seen in the adjacent muscle tissue. Moreover, the highest amounts of ALA‐induced porphyrins in the tumor and skin tissues were found 1 hr after i.p. injection, whereas the amounts of the porphyrins in both tissues increased with increasing time after topical application of ALA. The fluorescence of the porphyrins was localized in several components of the skin tissue (epidermis, hair follicles and their associated sebaceous glands). Furthermore, the fluorescence was diffusely distributed in the s.c. transplanted tumor tissue. Little could be observed under a confocal laser scan microscope (CLSM) in the muscle tissue. The uptake and biolocalization data correlate well with the results of PCT efficiency of the same tumor model with ALA‐induced porphyrins. Light and electron microscopy showed that the mitochondria of the tumor cells and of the endothelial cells and the basal lamina of vascular walls beneath the endothelium in the tumor tissue were initially extensively destroyed after PCT with ALA‐induced porphyrins. Thereafter, diffuse degeneration followed by local and/or diffuse severe necrosis of the tumor cells was found. This may be due mainly to the initial damage to mitochondria in the cancerous and endothelial cells and also to the destruction of the vascular wall in the tumor tissue. © 1992 Wiley‐Liss, Inc.
Spontaneously transformed rat‐liver epithelial‐cell line producing autocrine and paracrine growth factors
Authors: Narita Takeo 
Published: February 2007

AbstractA spontaneously transformed rat‐liver epithelial‐cell line that could proliferate in unsupplemented, serum‐free medium and be passaged with trypsinization has been established. At the time of writing, the line has undergone more than 100 passages in serum‐free culture. This cell line produced an autocrine growth‐stimulatory factor (AGSF) and a paracrine growth‐stimulatory factor (PGSF). AGSF had a remarkable growth‐stimulatory effect on transformed rat‐liver epithelial‐cells, but had no such effect on non‐transformed rat‐liver epithelial‐cells that could not proliferate in serum‐free medium. AGSF did not show a growth‐inhibitory or stimulatory effect on normal rat kidney (NRK) cells in monolayer culture, and did not induce anchorage‐independent growth in soft‐agar culture even with epidermal growth factor (EGF) or transforming growth factor (TGF)‐p. AGSF was protease‐sensitive, but heat‐ and acid‐stable. The molecular weight was about 700 Dalton (Da) on size‐exclusion chromatography. PGSF showed a growth‐stimulatory effect on NRK cells and induced anchorage‐independent growth in soft‐agar culture without EGF or TGF‐β. On the other hand, PGSF slightly inhibited the growth of the spontaneously transformed rat‐liver epithelial cells. PGSF was heat‐, protease‐ and acid‐sensitive. The molecular weight was 30 kDa on size‐exclusion chromatography. © 1992 Wiley‐Liss, Inc.
Specific growth stimulation in the absence of specific cellular adhesion in lung colonization by retinoic‐acid‐treated F9 teratocarcinoma cells
Authors: Rusciano Dario , Lorenzoni Patrizia , Burger Max M. 
Published: February 2007

AbstractIn most studies concerning organ‐specific metastasis, different or selected lines are compared with one another. Here we report results with the same cell line (the F9 murine teratocarcinoma) which can be directed towards liver or lung, depending on whether or not it is treated with retinoic acid and cyclic AMP. Thus, organ‐specific colonization by tail‐vein‐injected murine F9 teratocarcinoma cells shows a particular pattern: unselected, undifferentiated F9 cells preferentially colonize the liver of the syngeneic animal. The lungs, the first capillary bed encountered by cells thus injected, are only very rarely colonized. By contrast, the lungs become the main target organ of F9 cells induced to differentiate by treatment with retinoic acid and cyclic AMP. We have recently shown that liver colonization by undifferentiated F9 cells correlated with the adhesiveness of the cells (higher to fibronectin and liver‐derived extracellular matrix than to laminin and lung‐derived extracellular matrix) as well as with their growth response to organ‐derived extracts (no response with lung extracts and good response with liver extracts). The data reported below indicate that induction of differentiation causes at most a decreased adhesiveness of F9 cells to all the substrata tested (laminin, fibronectin, type‐IV collagen, organ‐derived extracellular matrix), suggesting that the shift in organ colonization observed with differentiated F9 cells is not due to an enhancement of the specific adhesion to the lung matrix. On the other hand, differentiated cells, but not undifferentiated ones, were able to respond to growth stimulation mediated by lung‐derived extracts, thereby implying a relevant role for organ‐specific growth stimulation in lung colonization by differentiated F9 cells. © 1992 Wiley‐Liss, Inc.
Activation of c‐fos and c‐fes in metastatic lympho‐macrophage hybrids
Authors: Fumagalli Stefano , Volpi Ludovica , Rossi Daniela , Larizza Lidia 
Published: February 2007

AbstractThe expression of proto‐oncogenes involved in myelomonocytic differentiation was studied in 3 metastatic murine lympho‐macrophage hybrids (ESb, EbF1, EbF2‐C4) which display several antigenic and functional macrophage properties. Constitutive expressions of fos and fesmRNA, which were not detectable in the weakly metastatic parent lymphoma (Eb), was shown in all the proliferating hybrid lines. By contrast, c‐myc and c‐myb expressions were shared by the tumor parent and the hybrids. The c‐fos transcripts observed in the hybrids were atypical in size, 2.3 kb, but standard 2.2‐kb transcripts could be detected in serum‐induced cells, even in the non‐expressing Eb lymphoma. The kinetics of the induced fos mRNA was found to conform to the features reported in the literature for a large variety of cells. No structural alteration of the c‐fos gene was detected by Southern analysis. PCR amplification of the 2.3‐ and 2.2‐kb fos mRNA showed that they are identical in size in both the coding and the 3′ untranscribed region. A longer poly A tail is thought to account for the additional 100 bp in the 2.3‐kb fos mRNA, as both the 2.3‐ and 2.2‐kb transcripts attain the same size following de‐adenylation. © 1992 Wiley‐Liss, Inc.
Human melanoma cell‐derived factor(S) stimulate fibroblast glycosaminoglycan synthesis
Authors: Edward Michael , Grant Allison W. , Mackie Rona M. 
Published: February 2007

AbstractConditioned media from cultures of human metastatic Hs294T melanoma cells contain a factor/factors that promote(s) fibro‐blast‐mediated contraction of collagen lattices, and stimulate(s) fibroblast glycosaminoglycan (GAG) synthesis. Complete medium from melanoma cell cultures stimulated fibroblast hyaluronate synthesis 9.3‐fold, and sulphated GAG synthesis 2.6‐fold, as measured by 3H‐glucosamine incorporation. 35SO4 incorporation into sulphated GAGS was essentially unaltered, the net result being a decrease in the degree of sulphation. Fibroblasts synthesized hyaluronate with an increased molecular weight when grown in the presence of the melanoma‐cell culture medium, while the molecular weights of heparan and chondroitin sulphates remained essentially unaltered.Our results indicate that the tumour‐cell‐derived factor(s) stimulate(s) changes in fibroblast glycosaminoglycan synthesis, and that these changes may facilitate tumour cell invasion in vivo. © 1992 Wiley‐Liss, Inc.
Prevalence of the rhesus‐negative phenotype in caucasian patients with small‐cell lung cancer (SCLC)
Authors: Cerny T. , Fey M. F. , Oppliger R. , Castiglione M. , Nachbur B. , Gertsch M. , Gasser A. , Joss R. A. , Thatcher N. , Lind M. , von Rohr A. , Nydegger U. E. 
Published: February 2007

AbstractWe report that the Rhesus (Rh)‐negative phenotype is more prevalent in patients with small‐cell lung cancer (SCLC) than in the normal Caucasian population (SCLC: 25% Rh‐negative vs. 15% expected, p < 0.0001). This finding has been validated for a Central and a Northern European population (Switzerland and UK). In contrast, the Rh‐negative phenotype is no more frequent in non‐small‐cell lung cancer patients or in heavy smokers with coronary heart disease than in the general population. There was a normal distribution of the ABO blood group phenotype in all patients studied. Whilst the significance of this observation is unclear, we hypothesize that a genetic predisposition to the development of SCLC may be linked to a hitherto unidentified gene on chromosome 1 p near the Rh locus. Our observation may perhaps allow further progress to be made in understanding genetic mechanisms of SCLC carcinogenesis. © 1992 Wiley‐Liss, Inc.
Insulin resistance and breast‐cancer risk
Authors: Bruning Peter F. , Bonfrèr Johannes M. G. , van Noord Paul A. H. , Hart Augustinus A. M. , de Jong‐Bakker Maartje , Nooijen Wim J. 
Published: February 2007

AbstractLife‐style has a major influence on the incidence of breast cancer. To evaluate the effects of life‐style related metabolic‐endocrine factors on breast cancer risk we conducted a case‐control study comparing 223 women aged 38 to 75 years presenting with operable (stage I or II) breast cancer and 441 women of the same age having no breast cancer, who participated in a population‐based breast cancer screening program. Women reporting diabetes mellitus were excluded. Sera from 110 women of the same age group presenting with early stage melanoma, lymphoma or cervical cancer were used as a second ‘other‐cancer control group’.Serum levels of C‐peptide were significantly higher in early breast cancer cases compared to controls. The same was found for the ratios C‐peptide to glucose or C‐peptide to fructosamine, indicating insulin resistance. Sex hormone binding globulin was inversely, triglycerides and available estradiol were positively related to C‐peptide. Serum C‐peptide levels were related to body mass index (BMI), and to waist/hip ratio (WHR), in particular in controls. However, the relative increase of C‐peptide, C‐peptide to glucose or C‐peptide to fructosamine in cases was independent of BMI or WHR. The log relative risk was linearly related to the log C‐peptide levels. Relative risk according to quintiles, and adjusted for age, family history, BMI and WHR, for women at the 80% level was 2.9 as compared with those at the 20% level for C‐peptide. Elevated C‐peptide or C‐peptide to fructosamine values were not observed in the sera from women belonging to the ‘other‐cancer control group’.This study suggests that hyperinsulinemia with insulin resistance is a significant risk factor for breast cancer independent of general adiposity or body fat distribution. © 1992 Wiley‐Liss, Inc.
Descriptive epidemiology and survival analysis of nasopharyngeal carcinoma in the united states
Authors: Burt Richard D. , Vaughan Thomas L. , McKnight Barbara 
Published: February 2007

AbstractPopulation‐based incidence data on nasopharyngeal carcinomas (NPC) from the Surveillance, Epidemiology and End Results program in the United States were examined for the years 1973–1986. The 1,645 cases showed incidence rates varying according to ethnic origin, with Chinese having the highest, followed by Filipinos, Blacks and Whites. In females the incidence was about half of that seen in males, in each of these groups. There was no evidence of changes in incidence rates during the study period. An analysis of survival with NPC was undertaken using hazard function regression models to allow for control of multiple variables. Survival was found to be independently influenced by age and stage at diagnosis, histologic type, grade and sex. Significant ethnic differences in survival remained after adjustment for these factors, with Chinese surviving longest followed by Filipinos, Whites and Blacks. These differences in survival remained after control for the variation in population‐wide mortality rates associated with age, ethnicity, sex and calendar year. We present evidence that survival has improved for this disease over time. © 1992 Wiley‐Liss, Inc.
Risk factors for renal‐cell cancer in Shanghai, China
Authors: McLaughlin Joseph K. , Gao Yu‐Tang , Gao Ru‐Nie , Zheng Wei , Ji Bu‐Tian , Blot William J. , Fraumeni Joseph F. 
Published: February 2007

AbstractA population‐based case‐control study of 154 histologically verified renal‐cell cancer patients and 157 controls was per formed in Shanghai, China, an area with low rates for this tumor. Elevated risks were observed for cigarette smoking (odds ratio (OR) = 2.3; 95% confidence interval (CI): 1.1 to 4.9), and for increasing categories of body weight and meat consumption, while reduced risks were seen for increasing categories of fruit and vegetable intake. An increased risk was also observed for regular use of phenacetin‐containing analgesics (OR = 2.3; 95% CI:0.7 to 7.0). These findings are consistent with earlier studies in Western countries, and indicate that many of the same etiologic factors for renal‐cell cancer operate in low‐and high‐risk societies. © 1992 Wiley‐Liss, Inc.
Frequent loss of heterozygosity on chromosomes Xp and 13q in human ovarian cancer
Authors: Yang‐Feng Teresa L. , Li Shibo , Han Hong , Schwartz Peter E. 
Published: February 2007

AbstractLoss of heterozygosity (LOH) was examined at 27 loci on chromosomes 3p, 6q, 11p, 13q, 17 and X in 42 human ovarian tumors. LOH was detected in 12 of 26 (46%) and 5 of 12 (42%) informative cases at 2 chromosome 13q loci, D13S32 and D13S34 respectively. On chromosome Xp, tumor‐specific allele loss was observed in 9 out of 15 informative cases (60%) at the ornithine transcarbamylase (OTC) gene locus. Examination of 12 additional Xp and 13q loci has mapped the common deletion regions to Xp21.1 → p11.4 and 13q33 → q34. The observation of significant LOH on Xp represents a strong indication of genetic changes in the X chromosome in a human malignancy. The allele losses on 13q which have been reported for other cancers suggest that chromosome 13, in addition to the retino blastoma gene, may contain other growth‐regulating gene(s) important in the development of several tumor types, including ovarian malignancies. © 1992 Wiley‐Liss, Inc.
Immunohistochemical determination of androgen receptors in relation to oestrogen and progesterone receptors in female breast cancer
Authors: Kuenen‐Boumeester V. , van der Kwast Th. H. , van Putten W. L. J. , Claassen C. , van Ooijen B. , Henzen‐Logmans S. C. 
Published: February 2007

AbstractThe expression of oestrogen (ER), progesterone (PR) and androgen (AR) receptors in female breast cancer was investigated by immunohistochemistry on snap‐frozen tissue specimens of a series of 100 breast cancers. For detection of the AR we used a recently developed mouse monoclonal antibody specific for the N‐terminal domain of the human AR. Expression of AR was compared with that of ER and PR as well as with tumour grade and age. Of the breast cancers investigated, 76% were AR‐positive. This high percentage corresponds well with previous data on AR expression in breast cancer determined with ligand‐binding assays. In 53% of the tumours AR, ER and PR were present, while 9% of the tumours were positive for AR and negative for ER and PR. In 13% of the tumours no ER, PR or AR expression was seen; these were all grade‐III tumours. A positive correlation was found between age and ER expression, but no correlation was seen between age and PR or AR. Future studies should establish the prognostic value of the combination ER, PR and AR determinations on female breast cancer with regard to biological behaviour and response rate to hormonal therapy. © 1992 Wiley‐Liss, Inc.
Involvement of histo‐blood‐group antigens in the susceptibility of colon carcinoma cells to natural killer‐mediated cytotoxicity
Authors: Blottiére Hervé M. , Burg Cédric , Zennadi Rahima , Perrin Pascale , Blanchardie Philippe , Bara Jacques , Meflah Khaled , Le Pendu Jacques 
Published: February 2007

AbstractThe susceptibility to natural‐killer‐cell lysis and expression of histo‐blood‐group antigens of 2 clones from a rat colon adeno‐carcinoma, of variants derived from them and of 17 human colon carcinoma cell lines were assessed in an attempt to determine if the major glycosidic tissue antigens of epithelial cells could influence the NK susceptibility of tumor target cells of epithelial origin. The rat REGb clone, which is relatively NK‐sensitive, expressed higher levels of precursor structures T and Tn and lower levels of H antigenic determinants than the PROb clone, which displays higher resistance to NK‐cell lysis. Cell variants were obtained from these 2 clones; it appeared that whether the cell variants were selected on the basis of expression of a blood‐group antigenic determinant or on the basis of altered susceptibility to NK‐cell lysis, there was a link between increased resistance and higher expression of cell‐surface A and H histo‐blood‐group antigens, or conversely, between increased sensitivity and higher expression of precursor structures. Similar conclusions were obtained upon study of the human cell lines, since a significant correlation was found between the level of expression of T or Tn antigens and sensitivity to NK‐cell lysis. A significant relationship was found between the expression of Lewis antigens and increased resistance to NK‐cell‐mediated cytotoxicity. © 1992 Wiley‐Liss, Inc.
Cytotoxicity of a transferrin‐adriamycin conjugate to anthracycline‐resistant cells
Authors: Fritzer Monika , Barabas Klara , Szüts Viktoria , Berczi Alajos , Szekeres Thomas , Faulk W Page , Goldenberg Hans 
Published: February 2007

AbstractConjugates of adriamycin coupled to transferrin by glutaraldehyde are cytotoxic to human promyelocytic (HL‐60) and erythroleukemic (K562) cells. Growth inhibition of adriamycin‐sensitive cells, as evaluated by thymidine incorporation and the MTT‐assay, was higher for conjugates than for free adriamycin. The cytotoxicity toward adriamycin‐resistant K562 and HL‐60 cells was 3‐fold and more than 10‐fold higher, respectively, for the transferrin‐adriamycin conjugate than for the free drug. The effect of the conjugate was dependent on its adriamycin content, i.e., on its coniugation number. © 1992 Wiley‐Liss, Inc.