bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rabbit oviduct mucosa healing after accidental transmucosal suture passage
Authors: Vuillemin Mauricette , Pexieder Tomas , Janecek Paul , Stamm Hans 
Published: March 2007

AbstractThe purpose of this study is to show by SEM the characteristics of mucosal healing related to the presence of intraluminal sutures of nylon (nonabsorbable) or dexon (absorable) in the rabbit oviduct.Altogether, 30 animals with anastomosis in the isthmus were killed 2, 4, 8, or 12 weeks after the operation. Tubular structures or pieces of thread partially or completely covered by the epithelium were found n 36.8% of these cases. Independently of the suture material used, already 2 weeks after surgery, the thread was covered by an epithelium composed mostly of squamous cells and some rare ciliated cells. At 4 weeks, the proportion of ciliated cells was increased. Regions with the usual cellular morphology and repartition of the different cellular types were also observed. However, at 8 and 12 weeks, islets of atypical squamous cells persisted in areas of transmucosal passage of the suture material. At 12 weeks, the dexon suture was not yet completely absorbed.
Sodium hyaluronate as an aid in microvascular surgery
Authors: Arnbjörnsson Einar O. 
Published: March 2007

AbstractThe purpose of this study was to compare the suture of a microvascular anastomosis with and without the aid of sodium hyaluronate. The divided femoral arteries of ten Sprague‐Dawley rats were sutured using sodium hyaluronate on one side. Operating time, bleeding, and patency rates were studied and compared. No significant differences were found in the measured parameters. However, the clinical impression is that the use of sodium hyaluronate facilitates the suture of a microanastomosis.
Experimental study of bridging the peripheral nerve gap with skeletal muscle
Authors: Jiming Kong , Shizhen Zhong , Bo Sun , Shengxiu Zhu 
Published: March 2007

AbstractA piece of skeletal muscle was used as a conduit to bridge the gap microsurgically between the two ends of the severed sciatic nerve in 30 rats, and a method for histochemical demonstration of acetylcholinesterase was employed to assess the nerve regeneration process 5 weeks or more after the operation. Regenerating nerve fibers were detected in the muscle and could be traced through to the distal stump of the severed sciatic nerve. This suggests that skeletal muscle might serve as a grafting conduit for the repairing of peripheral nerve injuries.
Dynamic computer tomography determining the patency of buried free flaps
Authors: Fischer Joseph C. , Parker Paul M. , Shaw William W. , Colen Stephen R. 
Published: March 2007

AbstractNumerous methods exist for monitoring the patency of cutaneous free flaps. Monitoring the patency of microvascular anastomosis in buried free flaps are limited. The present case report is of a 39‐year‐old female with Rhomberg's disease who underwent an abdominus rectus muscle free flap to correct the contour deformity of the right face. Dynamic CT scan was performed on the sixth postoperative day. Plots of the rate of change of density of the free flap were compared to an adjacent muscle of the face.Dynamic CT scanning provides a high degree of resolution of the free flap, the ability to examine the entire free flap, and the ability to determine the patency of buried free flap.
Scanning electron microscopy of crush/avulsion arterial trauma: Effect of heparin and aspirin administration
Authors: Cooley Brian C. , Ruas Ernesto J. , Wilgis E. F. Shaw 
Published: March 2007

AbstractA model of vascular trauma and subsequent reanastomosis with poor postoperative likelihood of patency was developed in the rat femoral artery. Patency rates were significantly improved with intravenous heparin, intragastric aspirin, and both agents together. Heparin yielded higher patency than aspirin. Intimal surfaces of the vessels at various postoperative intervals were observed with the scanning electron microscope. More fibrin accumulation was seen in the aspirin‐treated animals, while more platelet aggregation was found in the heparin‐treated group. Both platelet aggregation and fibrin strand development appeared retarded with both agents administered. All groups demonstrated good healing of the ruptured intimal surfaces, beginning at 2 days postoperatively. It is concluded that fibrin strand development is a more significant factor in microvascular occlusion than platelet aggregation.
Evaluation of long‐term patency rates of different techniques of arterial anastomosis in rabbits
Authors: Siemionow Maria 
Published: March 2007

AbstractThe results of 160 arterial anastomoses performed with four different techniques are presented. The popliteal arteries (mean diameter 0.7 mm) of 40 rabbits in each of four groups were anastomosed using: I. End‐to‐end techique with assymetrical sleeving of the adventitia; II. end‐to‐end technique with symetrical sleeving of the adventitia and wrapping of the suture site with a collagen cuff; III. end‐to‐end technique with symetrical trimming of the adventitia; and IV. end‐in‐end technique. Long‐term patency rates for the above techniques were as follows: Group I, 92.5%; Group II, 87.5%; Group III, 92.5%; Group IV, 90.0%. Group IV anastomoses were completed in an average of 15 minutes compared with an overall average of 24 minutes for the other three groups.
Microvascular polytetrafluoroethylene (Gore‐Tex®) grafts in the infrarenal rat aorta
Authors: Ribbe Else B. , Holmin Torsten , Löwenhielm Peter C. G. 
Published: March 2007

AbstractTo evaluate the use of microvascular prosthetic grafts, the infrarenal aorta in 33 male Sprague‐Dawley rats was replaced by an interposition graft of PTFE (polytetrafluoroethylene, Gore‐Tex®).Three groups of experimental animals were studied: Group A consisted of rats with 7‐mm‐long grafts, group B consisted of rats with 20‐mm‐long grafts, and group C consisted of rats with 20‐mm‐long grafts and ligatures of one common iliac and one external iliac artery. In group A all grafts (11/11) were patent after varying observation periods (6–92 days, median value 28 days, mean value 49 days). In group B all grafts but one (13/14) were patent (0–201 days, median value 198 days, mean value 118 days). In group C two grafts occluded immediately postoperatively, whereas the remaining six were patent (0–24 days, median and mean values 9 days). Twelve of 13 grafts observed for 3 months or more remained patent. There were no signs of infection. Angiography did not reveal any stenosis in the anastomoses of patent grafts. Light microscopy demonstrated a good adaptation between the grafts and the aorta in all animals. In the short grafts observed for 3 months and in the long grafts observed for 6 months, the luminal surfaces were completely covered by endothelial‐like cells. In the occluded graft in group B, a stenosis was demonstrated in one of the anastomoses. This was not found in any other specimen. The results of this study document the possibility of using PTFE grafts of 1 mm diameter in experimental microvascular position.
Allogeneic heart‐lung transplantation in mice
Authors: Chang Eil Sung , Chisari Francis V. , Lee Sun 
Published: March 2007

AbstractUsing a simpler technique for mouse heart and lung transplantation, a histological comparison was made between groups of syngeneic and allogeneic transplants. The initial high postoperative mortality encountered was due to technical difficulty and as the operative technique improved, mortality lessened. While direct palpation of the abdomen is the key means in the detection of graft rejection, there is no practical determination of such graft rejection other than histological clarification. Doppler tracing also is not a sensitive method for detecting rejection. The transplanation technique in this study compares the time of rejection of heart and lung transplants more legitimately.
Ovum recovery after microsurgical reanastomosis of the rabbit oviduct
Authors: Land Jolande A. , Evers Johannes L. H. , Boeckx Willy D. , Muytjens Arno , Brosens Ivo A. 
Published: March 2007

AbstractCauses for failure of pregnancy after tubal reconstructive surgery are incompletely understood. The impact of microsurgical resection reanastomosis on ovum recovery was studied in the rabbit oviduct. One hundred sixty rabbits were divided into three groups: one experimental group in which a resection reanastomosis was made in the ampullar or isthmic tubal segment, respectively, and two control groups. After mating, ovum recovery was evaluated. In ampullary operated oviducts the ovum recovery rate was significantly lower compared with recovery rates in isthmically operated and in control oviducts. Furthermore, in ampullary operated oviducts peritoneal transmigration of ova was frequently observed. Disturbances in ovum pickup from the ovarian surface or the peritoneal cavity seem to be responsible for the decrease in recovery rate found in the ampullary operated oviducts.
Microsurgical reconstruction of congenital tubal anomalies
Authors: Cohen Brian M. 
Published: March 2007

AbstractSignificant numbers of infertile women have congenital anomalies or anatomical distortion of the fimbrial‐gonadal mechanism in their otherwise healthy oviducts. The diagnosis and surgical treatment of multiple congenital accessory tubal ostia, the elongated fimbria‐ovarica syndrome, and management of paratubal cysts is described. Microsurgical correction of these defects was followed by term pregnancies in 46%, 57% and 28% of the three entities respectively and this data indicates that significant numbers of women with these defects may be helped by microsurgery. Failure to recognize these abnormalities may otherwise result in patients being diagnosed as idiopathic infertility, a decision which may exclude them from the possibility of undergoing a successful form of microsurgical treatment.
Comparison between tubouterine implantation and tubouterine anastomosis for repair of cornual occlusion
Authors: Fayez Jamil A. 
Published: March 2007

AbstractForty‐four patients with partial or complete cornual occlusion of their tubes had undergone microsurgical repair in the form of either tubouterine implantantion or tubouterine anastomosis. Eighteen of these patients had undergone the implantantion procedure and 26 the anastomosis procedure. None of the patients in the implantation group had previous tubal sterilization, while in the anastomosis group 16 had tubal cauterization and 10 had other causes blocking the cornual end of their tubes. The patency rate in the implantation group was 70% and the pregnancy rate was 39%, while in the anastomosis group the rates were 94% and 69%, respectively. It is concluded that, when feasible, tubouterine anastomosis should be the procedure of choice for the repair of the cornual occlusion of the tube irrespective of the cause of obstruction.
Microsurgical laser technique for abdominal myomectomy
Authors: Reyniak J. Victor , Corenthal Linda 
Published: March 2007

AbstractThis study was designed to evaluate the applicability of a hand‐held CO2 laser combined with microsurgical principles to the abdominal myomectomy in women with menorrhagia and infertility. Seventeen patients with submucous and intracavitary myomata diagnosed by hysterosalpingography were included in this study. The myomectomy technique described was rapid, allowed for excellent intraoperative hemostasis, and resulted in a short, uneventful postoperative hospital stay. In all patients, menstrual pattern returned to normal. Seven out of ten patients who attempted pregnancy succeeded.
Laparoscopic assessment of ovarian healing following CO2 laser microsurgery: Vicryl vs. Surgilon
Authors: McLaughlin David S. , Diamond Michael P. , Daniell James F. , Martin Dan C. , Feste Joseph 
Published: March 2007

AbstractA prospective multicenter study was undertaken by the Intraabdominal Laser Study Group to assess the type and extent of ovarian adhesions formed following incisional ovarian surgery performed as part of a fertility‐promoting procedure with the aid of the carbon dioxide laser. Ovarian healing with subsequent adhesion formation was evaluated by early second‐look laparoscopy. Two suture closing materials were used—Surgilon (siliconized braided nylon, David and Geck, Wayne, NJ) and Vicryl (polyglactin 910, Ethicon, Somerville, NJ). Sixty‐five ovaries were evaluated in 37 patients with subsequent adhesions found in 37% (18/49) of the Surgilon‐closed ovaries and in 94% (15/16) of the Vicryl‐closed ovaries. The adhesions were filmy in 83% (15/18) of the Surgilon group and 47% (7/15) of the Vicryl group. Densel vascular adhesions were found in 17% (3/18) of the Surgilon group and 53% (8/15) of the Vicryl group. The subsequent conception rates were 68% (17/25) in the Surgilon group and 33% (4/12) in the Vicryl group, although the small number of patients and multitude of infertility factors made pregnancy rate comparisons difficult. One of the authors noted lack of tensile strength in the Vicryl‐closed ovaries within 12 weeks postlaser laparotomy, which led to incisional dehiscence during laparoscopic lysis of ovarian adhesions. From this preliminary study, it is concluded that additional data are needed to more fully resolve the debate on whether absorbable or nonabsorbable suture is preferred for ovarian infertility surgery.
Pathogenesis of adhesion formation/reformation: Application to reproductive pelvic surgery
Authors: Diamond Michael P. , Decherney Alan H. 
Published: March 2007

AbstractPelvic adhesions are a major contributing factor to infertility in women with tubal peritoneal disease. Surgical treatment of such adhesions often does not resolve their presence. Such postoperative adhesion formation occurs despite the use of microsurgical techniques in a wide variety of surgical adjuvants. The process of adhesion formation represents a variation of the normal healing process of the peritoneum. However, rather than normal mesothelial healing, the fibrinous mass resulting from the peritoneal defect is infiltrated by fibroblasts with subsequent adhesion formation. A description of the pathophysiology of this process is described. The current understanding of the pathologic/pathophysiologic interaction of the surgical adjuvants with this process is described.
Use of streptokinase for the salvage of a free flap: Case report and review of the use of thrombolytic therapy
Authors: Schubert Warren , Hunter David W. , Guzman‐Stein Gabriela , Ahrenholz David H. , Solem Lynn D. , Dressel Thomas D. , Cunningham Bruce L. 
Published: March 2007

AbstractThis is the first case report of the clinical use of intraoperative streptokinase to promote free flap salvage. A latissimus dorsi free flap was mobilized to cover a scalping type injury. After 4 1/2 hours of ischemia and recurrent thrombosis, streptokinase was perfused into the thoracodorsal artery (7,500 units of streptokinase in 30 cc of normal saline). The free flap was exposed to this concentration of streptokinase for 10 minutes followed by drainage of the venous effluent in order to avoid possible deleterious systemic effects of the streptokinase. Good flow throughout the free flap resulted, and the flap remained viable, providing good coverage for the patient's skull. Controversies regarding the no‐reflow phenomena and the use of various thrombolytic agents are discussed.
Mural repair following obliteration of aneurysms: Production of experimental aneurysms
Authors: Young Paul H. , Fischer Vernon W. , Guity Afrassiab , Young Paul A. 
Published: March 2007

AbstractOne hundred thirty‐two experimental rat carotid artery aneurysms were produced using a variety of methods, in order to determine which of these approaches should be employed in the development of a satisfactory laboratory model. The gross appearance and pathomorphology of aneurysms created by vein pouch and partial arteriotomy methods most closely resembled those features reported in clinical intracranial aneurysms.
Second toe transfer for thumb reconstruction in multiple digit amputations including thumb and basal joint
Authors: Tsai Tsu‐Min , McCabe Steve , Beatty Mary Ellen 
Published: March 2007

AbstractAmputation of the thumb, including the basal joint, is a disabling injury that leaves limited reconstructive options.Three cases of multiple digit amputation including the thumb and basal joint are presented in which a second toe transfer was used to reconstruct the thumb.All patients have functional pinch with an average pinch strength of 27% of the opposite uninjured extremity. The average grip strength for two patients is 44% of the opposite hand. Average motion of the transfer at the MP, PIP, and DIP is 25°, 37°, and 9°, respectively. Adduction and abduction are obtained, the degree depending on the site of proximal bony fixation. No patient has morbidity at the donor site and all are happy with their reconstruction.In selected cases we feel second toe transfer is an excellent choice to reconstruct the thumb amputated at a proximal level.
The wraparound procedure for thumb and finger reconstruction
Authors: Lowdon Ian M. R. , Nunley James A. , Goldner Richard D. , Urbaniak James R. 
Published: March 2007

AbstractNineteen thumb and finger reconstructions were performed using the wraparound procedure; there were five early arterial failures. Thirteen of the 14 successful digital reconstructions were reviewed at an average of 26 months after surgery. There were complications related to the upper limb in seven patients, several of whom had more than one problem; these included radial skin breakdown (2), malrotation (2), and infection (2). One patient underwent late deletion for chronic infection following corrective osteotomy. Time until toe donor site healing averaged 2 months. We considered that cosmesis and function were good. Pinch grip averaged 48% of normal; sensibility returned in all patients—four regained protective sensation and the others attained two–point discrimination (average 9 mm). The wraparound procedure provides a cosmetically and functionally excellent method of thumb reconstruction for amputation distal to the metacarpophalangeal joint.
Free neurovascular cutaneous toe pulp transfer for thumb reconstruction
Authors: Stern Peter J. 
Published: March 2007

AbstractFree vascularized toe pulp transfer to the thumb is a technique to restore sensibility and durable soft tissue coverage to the thumb. This article reviews alternative techniques for coverage; the neurovascular anatomy of the great toe; and the technique of microvascular toe pulp transfer. Although the procedure is technically demanding, it provides excellent coverage with minimal donor morbidity.
Neurovascular island skin flaps in the hand: Functional and sensibility evaluations
Authors: Stice R. Colleen , Wood M. B. 
Published: March 2007

AbstractThe value of neurovascular island skin flap transfers for digit tip surfacing has been evaluated in 13 patients. A computer assisted sensory evaluation was carried out on six of the patients. All patients were satisfied with the procedure. Sensory acuity did not appear to diminish with time and sensory misreference was not a major functional problem. We suggest that the neurovascular island flap, when available, is still an excellent procedure for restoration of useful digit tip sensibility.
A model for sidewall adhesions in the rabbit: Reduction by an absorbable barrier
Authors: Diamond Michael P. , Linsky Cary B. , Cunningham Tim , Constantine Barry , Dizerega Gere S. , Decherney Alan H. 
Published: March 2007

AbstractDespite use of surgical adjuvants, pelvic adhesions frequently develop following infertility surgery. Recently a resorbable biocompatible material, TC 7, has been designed to be used as a barrier to reduce adhesion formation. Reproducibly severe sidewall adhesions were only able to be created in one of six models tested. The model consisted of the following steps: excision of a full thickness 2 × 2‐cm musculoperitoneal tissue mass, scraping of an adjacent 2cm length of uterine horn, tamponading of all bleeding, and suturing (6‐0 Vicryl) of normal tube and sidewall so as to directly approximate the traumatized tissues. Studies were conducted in 13 rabbits after creation of the bilateral sidewall and horn lesions, each serving as its own control. Choice of control and TC 7 sides was determined randomly. Adhesion formation was examined 2 weeks postoperatively. No residual material was noted at that time. Adhesion scores were the composite total of extent (0–4), type (0–4), and tenacity (0–3) of sidewall adhesions, and were significantly reduced on the TC 7 side as compared with the control side (mean 6.8 ± 0.4 vs 9.0 ± 0.3, median 6 vs 9, sign test P = 0.0032). No complications of use of the barrier were noted. It is concluded that use of TC 7, a resorbable biocompatible barrier, was able to significantly reduce postoperative adhesion formation on the rabbit sidewall.
Experimental microvascular autogenous vein grafts for arterial defects: A study of anastomotic sites
Authors: Kruavit Arthi , Fletcher Christopher D. M. , McKee Phillip H. , Mayou Brian 
Published: March 2007

AbstractAutogenous femoral vein grafts with an average external diameter of 1.5 mm and an average length of 2.87 cm have been used to bridge defects in the contralateral femoral artery of 15 adult New Zealand white rabbits. An experimental microvascular technique to minimise trauma to the graft was performed, by which clamps were never applied to the graft itself. Patency was assessed over a 12‐week period, and the overall graft success rate, excluding one technical failure, was 86%. Each of the three failures resulted from thrombosis. Histologic examination of the patent anastomoses showed marked medial damage at 1 week, complicated by fibrosis and calcification by 4 weeks; thereafter the intima developed prominent fibroelastic thickening. This led to a degree of luminal narrowing by 12 weeks. However, the underlying medial damage, attributable to operative trauma, did not seem to diminish luminal patency.
Experimental models for evaluating antithrombotic therapies in replantation microsurgery
Authors: Cooley Brian C. , Gould John S. 
Published: March 2007

AbstractA variety of anticoagulation and antiplatelet aggregation agents are in use clinically, and in applications to microvascular surgery, a clear choice for the most effective therapy has not been determined. Models of vascular trauma combined with microvascular anastomosis have been developed for comparing the efficacy of these agents in maintaining vascular patency. In a rat model of microvascular thrombosis, heparin effectively prevents occlusion in both arteries and veins, at clinical levels of administration. Aspirin helps prevent thrombosis, but not as well as heparin. These results support the beneficial effect of antithrombotic drug therapies, and suggest a more potent role of the heparininhibited fibrin clot over platelet aggregation in creating thrombotic occlusion of small vessels.
Experimental vasospasm
Authors: Seaber Anthony V. 
Published: March 2007

AbstractVasospasm is known to be present during most microvascular procedures. Not until recently has microsurgery made it possible to directly observe the dynamic nature of vascular spasm. This report reviews the physiologic events and the causes of vascular spasm during microsurgery. The current research models and the use of spasmolytic agents to relieve spasm are outlined. Future research in specific areas that pertain to the cremaster muscle model and the future direction and need for research in this area are discussed.