bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station
Authors: Ishii Hiroto , Endo Rin , Hamanaka Sanae , Hidaka Nobuyuki , Miyauchi Maki , Hagiwara Naho , Miyao Takahisa , Yamamori Tohru , Aiba Tatsuya , Akiyama Nobuko , Akiyama Taishin 
Published: March 2024

AbstractThe analysis of cells frozen within the International Space Station (ISS) will provide crucial insights into the impact of the space environment on cellular functions and properties. The objective of this study was to develop a method for cryopreserving blood cells under the specific constraints of the ISS. In a ground experiment, mouse blood was directly mixed with a cryoprotectant and gradually frozen at -80 °C. Thawing the frozen blood sample resulted in the successful recovery of viable mononuclear cells when using a mixed solution of dimethylsulfoxide and hydroxyethyl starch as a cryoprotectant. Additionally, we developed new freezing cases to minimize storage space utilization within the ISS freezer. Finally, we confirmed the recovery of major mononuclear immune cell subsets from the cryopreserved blood cells through a high dimensional analysis of flow cytometric data using 13 cell surface markers. Consequently, this ground study lays the foundation for the cryopreservation of viable blood cells on the ISS, enabling their analysis upon return to Earth. The application of this method in ISS studies will contribute to understanding the impact of space environments on human cells. Moreover, this method may find application in the cryopreservation of blood cells in situations where research facilities are inadequate.
Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 protein
Authors: Bohara Vijay Singh , Deshmukh Atharva , Kumar Sachin 
Published: March 2024

AbstractPyruvate kinase isoform M2 (PKM2) is a key modulator of glucose metabolism. While the major role of PKM2 is to facilitate the breakdown of glucose, it is potentially associated with other additional non-glycolytic functions as well. The role of PKM2 in the autoimmune response and inflammatory process is increasingly being acknowledged as a crucial modulator of cellular pathophysiological activity. However, its role in modulating viral replication has not been explored in detail. In the present study, we have shown a significant increase in endogenous PKM2 expression in JEV-infected mouse neuroblastoma cells. Furthermore, overexpression of PKM2 significantly reduced JEV replication, suggesting a negative effect of PKM2 on JEV replication. This was further confirmed by siRNA-mediated downregulation of endogenous PKM2 expression, which resulted in enhanced JEV replication. In silicostudies revealed the potential interaction between PKM2 and NS1 protein of JEV. The microscopic studies also showed cellular colocalization of PKM2 and NS1 in the ER of infected cells. The interaction was further validatedin vitroby co-immunoprecipitation assay. The present study suggests that PKM2 negatively regulates the JEV replication by its possible interaction with NS1.ImportanceJapanese encephalitis (JE) is a neuroinflammatory disease caused by the Japanese encephalitis virus (JEV). JE is a major threat to public health not only because it causes many deaths but also for its permanent neuropsychiatric sequelae in children. Out of all non-structural proteins of JEV, NS1 is highly immunogenic. A wide range of possible interactive partners has been identified for the NS1, many of those have been linked to immune evasion and regulating viral replication. In the current study, we have described a novel host cell factor, PKM2 modulating JEV replication by interacting with NS1 protein. Considering PKM2’s central role in regulating host cell metabolism, our findings suggest a previously unrecognized role for PKM2 in JEV neuropathogenesis. The identification and characterization of previously unknown host factors, as well as the elucidation of their regulatory mechanisms, are of utmost importance in the development of innovative treatments and antivirals against JEV.
Dynamic capabilities of global and local humanitarian organizations with emergency response and long-term development missions
Authors: Son Byung-Gak , Roscoe Samuel , Sodhi ManMohan S. 
Published: March 2024

PurposeThis study aims to answer the question: What dynamic capabilities do diverse humanitarian organizations have?Design/methodology/approachWe examine this question through the lens of dynamic capabilities with sensing, seizing and reconfiguring capacities. The research team interviewed 15 individuals from 12 humanitarian organizations that had (a) different geographic scopes (global versus local) and (b) different missions (emergency response versus long-term development aid). We also gathered data from secondary sources, including standard operating procedures, company websites, and news databases (Factiva, Reuters and Bloomberg).FindingsThe findings identify the operational and dynamic capabilities of global and local humanitarian organizations while distinguishing between their mission to provide long-term development aid or emergency relief. (1) The global organizations, with their beneficiary responsiveness, reconfigured their sensing and seizing capacities throughout the COVID-19 pandemic by pivoting quickly to local procurement or regional supply chains. The long-term development organizations pivoted to multi-year supplier agreements with fixed pricing to counter price uncertainty and accessed social capital with government bodies. In contrast, emergency response organizations developed end-to-end supply chain visibility to sense changes in supply and demand. (2) Local humanitarian organizations developed the capacity to sense demand and supply changes to reconfigure based on their experiential learning working with the local community. The long-term-development local organizations used un-owned and scalable relief infrastructure to seize opportunities to rebuild affected areas. In contrast, emergency response organizations developed their capacity to seize opportunities to provide aid stemming from their decentralized decision-making, a lack of structured procedures, and the authority for increased expenditure.Originality/valueWe propose a theoretical framework to identify humanitarian organizations' operational and dynamic capabilities, distinguishing between global and local organizations and their emergency response and long-term aid missions.
Impact of educating faculty on student assessment: beyond satisfaction level
Authors: Mohamed Wessam 
Published: March 2024

PurposeThis study evaluated the impact of a faculty training program on student assessment using the Kirkpatrick model.Design/methodology/approachA self-reported survey assessed 111 Saudi and non-Saudi participants' satisfaction. Subjective and objective measures (self-reported measures, assessment literacy inventory and performance-based assessment tasks) gauged participants' learning level. Pre- and post-training data were collected from 2020 to 2022.FindingsA highly significant effect on satisfaction (>80%) and learning levels was observed, as manifested by workplace practices of student assessment (>70%, the cut-off score). Pre- and post-training comparisons of participants' satisfaction and assessment literacy scores showed significant improvements following training. Multiple regression analyses showed no significant effects for gender and educational attainment but a substantial impact of academic cluster on participants' student assessment skills.Research limitations/implicationsLong-term effects of training faculty on assessment practices and student achievement will be studied at the institutional level in future research.Practical implicationsThe current study contributes to human capital investment via faculty training on student assessment, helping them comply with assessment best practices. This assures the quality, fairness and consistency of assessment processes across disciplines in higher education institutions, enhances assessment validity and trust in educational services and may support institutional accreditation.Social implicationsThis study provides opportunities for sharing best practices and helps establish a community of practice. It enhances learning outcomes achievement and empowers higher education graduates with attributes necessary to succeed in the labor market. The human capital investment may have a long-term impact on overall higher education quality.Originality/valueThis study contributes to the scarce literature investigating the impact of training faculty from different clusters on student assessment using subjective and objective measures. It provides developing and evaluating a long-term student assessment program following the Kirkpatrick model.
What is known about Australian child protection practitioners’ participation in the health care of children living in out of home care: a scoping review
Authors: Sanders Rachael Elizabeth , Modderman Corina , Bracksley-O'Grady Stacey , Harley Fiona , Spencer Jacquelin , Molloy Jacinta 
Published: March 2024

PurposeThere is a growing recognition of the urgency to enhance health outcomes for children and young people residing in out-of-home care (OOHC). Research underscores the need to establish effective pathways to quality health care for children and young people who have been exposed to trauma. Child protection (CP) practitioners should play a vital role in proactively improving health outcomes and navigating the intricacies of healthcare systems. Their involvement in initiating and collaborating on healthcare interventions is pivotal for the well-being of these vulnerable children and young people. However, challenges associated with poor health literacy and the complexities of healthcare systems hinder collaborative service delivery in the Australian context. This review explores how CP practitioners support the health care of children and young people in their care.Design/methodology/approachA scoping review followed Arksey and O’Malley’s framework, employing a narrative synthesis to assess the selected studies.FindingsHealth outcomes for children and young people in OOHC remain under-researched and potentially under-resourced within the realm of CP practice. There is room for enhanced practices and system integration in CP service delivery to better address health needs and prevent further health and well-being disparities.Originality/valueThrough this scoping review and involving industry experts in the discussion of findings, this study contributes valuable insights to the existing knowledge base regarding the active participation of CP practitioners in addressing the healthcare needs of vulnerable children.
Rosemary extract and rosmarinic acid accelerate elastic fiber formation by increasing the expression of elastic fiber components in dermal fibroblasts
Authors: Kasamatsu Shinya , Takano Kei , Aoki Mika , Takahashi Yoshito , Suzuki Tamio 
Published: March 2024

AbstractUltraviolet (UV)‐induced skin photoaging is caused by qualitative and quantitative degradation of dermal extracellular matrix components such as collagen and elastic fibers. Elastic fibers are important for maintaining cutaneous elasticity, despite their small amount in the skin. Previously, microfibril‐associated protein 4 (MFAP‐4), which is downregulated in photoaging dermis, has been found to be essential for elastic fiber formation by interaction with both fibrillin‐1 and elastin, which are core components of elastic fiber. In addition, enhanced cutaneous MFAP‐4 expression in a human skin‐xenografted murine photoaging model protects against UV‐induced photodamage accompanied by the prevention of elastic fiber degradation and aggravated elasticity. We therefore hypothesized that the upregulation of MFAP‐4 in dermal fibroblasts may more efficiently accelerate elastic fiber formation. We screened botanical extracts for MFAP‐4 expression‐promoting activity in normal human dermal fibroblasts (NHDFs). We found that rosemary extract markedly promotes early microfibril formation and mature elastic fiber formation along with a significant upregulation of not only MFAP‐4 but also fibrillin‐1 and elastin in NHDFs. Furthermore, rosmarinic acid, which is abundant in rosemary extract, accelerated elastic fiber formation via upregulation of transforming growth factor β‐1. This was achieved by the induction of cAMP response element‐binding protein phosphorylation, demonstrating that rosmarinic acid represents one of the active ingredients in rosemary extract. Based on the findings in this study, we conclude that rosemary extract and rosmarinic acid represent promising materials that exert a preventive or ameliorative effect on skin photoaging by accelerating elastic fiber formation.
Long‐term changes in stream water chemistry in small forested watersheds in the northern Kanto region
Authors: Urakawa Rieko , Toda Hiroto , Cao Yue 
Published: March 2024

AbstractConcerns have been raised regarding the degradation of stream water quality due to the excessive influx of atmospheric deposition. This study aimed to reveal the long‐term variation in stream water chemistry in approximately 40 forested small watersheds in the northern Kanto region, based on 3 surveys conducted in 1991–1992, 2006–2007, and 2022. The factors influencing each dissolved element were investigated. Regarding long‐term variations in stream water chemistry, relatively stable concentrations of cations (Ca2+, Mg2+, K+, and Na+) and SiO2 were observed. However, the concentrations of anions (Cl−, , and ) decreased due to the improvement in the atmospheric environment and the diminishing effect of fertilization. Furthermore, the recent increase in stream water pH was attributed to an increase in bicarbonate (), compensating for the decrease in anion concentrations relative to cations. Geology was the most significant factor for inter‐watershed variations in cations and SiO2, and this influence remained relatively constant over 30 years. Forest practices, especially fertilization, had a significant effect on Cl− and , and contributed to higher concentrations in the fertilized watersheds than in the non‐fertilized watersheds over the 15 years following fertilization. Sulfate deposition was a significant influencing factor for , and the concentration fluctuated under long‐term variations in deposition over the past 30 years. Despite substantial acid deposition in the study area, the absence of stream water acidification could be attributed to the abundant cation supply from bedrock and volcanic ash, which underwent weathering processes.
Socioemotional Wealth and Family Firm Performance: The Moderating Role of CEO Tenure and Millennial CEO
Authors: Åberg Carl , Calabrò Andrea , Valentino Alfredo , Torchia Mariateresa 
Published: March 2024

AbstractThis study sheds light on how socioemotional wealth (SEW) theory functions in family firms. Focusing on the impact of the most highly appraised FIBER dimensions on the performance of such firms, we contextualize SEW by discussing the heterogeneity among family principals via the under‐researched role played by specific characteristics of family CEOs. Integrating arguments from SEW and generational theory, we test our theoretical hypotheses using global survey data from a sample of 1833 family firms from 33 countries. The main findings suggest that while family control and influence is not associated with performance, family members’ identification with the firm (FI) improves performance. Moreover, the positive impact of FI on family firm performance weakens in family firms with long‐tenured family CEOs. Finally, in family firms led by Millennial CEOs, the positive impact of FI on family firm performance is stronger. Our findings enrich both the theoretical insights into and practical comprehension of SEW priorities in relation to family firm performance, thereby underscoring the diverse performance outcomes associated with various types of family CEOs.
A rare case of tumor‐to‐tumor metastasis from thymic carcinoma to an ovarian mature teratoma
Authors: Sasada Tsuyoshi , Shirakawa Chigusa , Tachikawa Ryo , Yamashita Daisuke , Tomii Keisuke 
Published: March 2024

AbstractMetastasis from one neoplasm to another is referred to as tumor‐to‐tumor metastasis (TTM). TTM is rarely observed. Here, we present a patient with TTM from a thymic carcinoma to an ovarian mature teratoma. A 25‐year‐old woman, diagnosed with unresectable thymic carcinoma, presented with a cyst with a solid tumor component in her right ovary. Laparoscopic cystectomy of the right ovary revealed that the solid tumor was a distant metastasis of the thymic carcinoma in an ovarian mature teratoma. The possibility of malignant transformation of the ovarian mature teratoma was ruled out, enabling accurate staging of the thymic carcinoma. This case emphasizes the need for clinicians to consider TTM and the importance of pathological confirmation of TTM when investigating potential distant metastases.
Diversity and function of fluorescent molecules in marine animals
Authors: Poding Lars H. , Jägers Peter , Herlitze Stefan , Huhn Mareike 
Published: March 2024

ABSTRACTFluorescence in marine animals has mainly been studied in Cnidaria but is found in many different phyla such as Annelida, Crustacea, Mollusca, and Chordata. While many fluorescent proteins and molecules have been identified, very little information is available about the biological functions of fluorescence. In this review, we focus on describing the occurrence of fluorescence in marine animals and the behavioural and physiological functions of fluorescent molecules based on experimental approaches. These biological functions of fluorescence range from prey and symbiont attraction, photoprotection, photoenhancement, stress mitigation, mimicry, and aposematism to inter‐ and intraspecific communication. We provide a comprehensive list of marine taxa that utilise fluorescence, including demonstrated effects on behavioural or physiological responses. We describe the numerous known functions of fluorescence in anthozoans and their underlying molecular mechanisms. We also highlight that other marine taxa should be studied regarding the functions of fluorescence. We suggest that an increase in research effort in this field could contribute to understanding the capacity of marine animals to respond to negative effects of climate change, such as rising sea temperatures and increasing intensities of solar irradiation.
Eculizumab use throughout pregnancy in two patients with aquaporin‐4‐positive neuromyelitis optica spectrum disorder
Authors: Fujimoto Takeshi , Maeda Yasuhiro 
Published: March 2024

AbstractBackgroundPatients with neuromyelitis optica spectrum disorder (NMOSD) are at an increased risk of pregnancy complications. Lack of NMOSD treatment during pregnancy is a risk factor for relapse. Here, we report two cases of pregnant women with anti‐aquaporin‐4 antibody‐positive (AQP4+) NMOSD treated with eculizumab during pregnancy.Case PresentationPatient 1 was diagnosed with AQP4+ NMOSD 2 mo after giving birth to her first child. She was treated with tacrolimus and prednisolone, before switching to prednisolone monotherapy. Following concerns of teratogenicity associated with immunosuppressive therapy and oral steroid use, she began eculizumab treatment prior to a second pregnancy. When she became pregnant, eculizumab treatment was briefly paused while safety data were reviewed with her neurologist. A total of three doses were missed.Patient 2 was diagnosed with AQP4+ NMOSD and began prednisolone treatment. Following a relapse, tacrolimus was added to her treatment regimen. Prior to pregnancy, she began eculizumab treatment alongside prednisolone and tacrolimus and maintained this regimen throughout her pregnancy.No relapses or meningococcal infections occurred after eculizumab initiation in either patient, and both gave birth without complications to healthy babies. Patient 2 continues to receive eculizumab while breastfeeding.ConclusionsWe present two cases of pregnant women with AQP4+ NMOSD treated with eculizumab. Both women gave birth to healthy babies, have had no relapses since initiating eculizumab, and continued their treatment after birth. These cases are further evidence of the successful use of eculizumab during pregnancy.
Optimal design of colour formulation prediction for cotton fabrics based on NSGA‐II and TOPSIS
Authors: Zhou Zeyan , Lin Zijian , Ma Yue , Niu JiaRong , Liu Jianyong , Wang Xiaoyin 
Published: March 2024

AbstractThe prediction of colour formulation is an important step in reproducing the target colour. At present, there are relatively few researches on multi‐objective colour formulation problem, and the colour matching accuracy needs to be improved. In this research, a multi‐objective evolutionary meta‐heuristic method based on the Fast and Elitist Multi‐objective Genetic Algorithm (NSGA‐II) was proposed to predict the target colour recipes. The method used dye concentration as a variable and included three objective functions: (1) minimising the CMC (Colour Measurement Committee) colour difference between the formulation colour and the target colour, (2) minimising the metamerism index, and (3) minimising the cost of the formulation. The algorithm could obtain the Pareto optimal solution set after iteration. On this basis, the best combination of formulations was selected from the optimal solution set by combining the Expert Scoring Method (ESM), Entropy Weight Method (EWM) and the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS). The prediction effect of the model was evaluated by taking cotton fabrics and reactive dyes actually used in plant as examples. The results showed that 87.5% of the formulations met the CMC colour difference value of no more than 1, the metamerism index of 90.0% of the formulations did not exceed 1, and the cost of 92.5% of the formulations was reduced relative to the maximum extent in the Pareto optimal solution set. Further studies should be focused on removing duplicate individuals to give better diversity in the Pareto optimal solution set.
Effects of transcutaneous auricular vagus nerve stimulation at left cymba concha on experimental pain as assessed with the nociceptive withdrawal reflex, and correlation with parasympathetic activity
Authors: Yokota Hirotake , Edama Mutsuaki , Kawanabe Yurika , Hirabayashi Ryo , Sekikne Chie , Akuzawa Hiroshi , Ishigaki Tomonobu , Otsuru Naofumi , Saito Kei , Kojima Sho , Miyaguchi Shota , Onishi Hideaki 
Published: March 2024

AbstractThe aim of this study was to clarify the effects of transcutaneous auricular vagus nerve stimulation (taVNS) to the left cymba concha on the pain perception using nociceptive withdrawal reflex (NWR), which is known to be associated with chronic pain, and to investigate whether there is a relationship between taVNS‐induced suppression of the NWR and parasympathetic activation. We applied either 3.0 mA, 100 Hz taVNS for 120 s on the left cymba concha (taVNS condition) or the left earlobe (Sham condition) for 20 healthy adults. NWR threshold was measured before (Baseline), immediately after (Post 0), 10 min (Post 10) and 30 min after (Post 30) stimulation. The NWR threshold was obtained from biceps femoris muscle by applying electrical stimulation to the sural nerve. During taVNS, electrocardiogram was recorded, and changes in autonomic nervous activity measured by heart rate variability (HRV) were analyzed. We found that the NWR thresholds at Post 10 and Post 30 increased compared with baseline in the taVNS group (10 min after: p = .008, 30 min after: p = .008). In addition, increased parasympathetic activity by taVNS correlated with a greater increase in NWR threshold at Post 10 and Post 30 (Post 10: p = .003; Post 30: p = .001). The present results of this single‐blinded study demonstrate the pain‐suppressing effect of taVNS on NWR threshold and suggest that the degree of parasympathetic activation during taVNS may predict the pain‐suppressing effect of taVNS after its application.