bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nachhaltiges Sanieren? Zur ökologischen Effektivität und ökonomischen Effizienz energetischer Sanierungsmaßnahmen im Altbau am Beispiel von Dachsanierungen
Authors: Messari‐Becker Lamia 
Published: October 2008

AbstractCa. 90 % der in den privaten Haushalten anfallenden CO2‐Emissionen werden im Altbau verursacht. Das macht Fortschritte in diesem Bereich unabdingbar, um die vorhandenen Potentiale zur CO2‐Minderung im Gebäudebereich zu mobilisieren. Die Steigerung der Energieeffizienz im Gebäudebereich ist zudem ein wesentlicher Schlüssel, um die gegenwärtigen Energieprobleme zu entschärfen. Die Politik verbindet mit den vorgesehenen Maßnahmen, wie z. B. die Anforderungen der EnEV im Bestand, auch die Minderung von CO2‐Emissionen. Deshalb sollten Sanierungsmaßnahmen im Altbau auch auf ihre Gesamteffektivität hinsichtlich der CO2‐Minderungseffekte bewertet und dementsprechend gefördert werden. Dieser Aufsatz vergleicht Varianten von Bauteilsanierungen hinsichtlich ihrer ökologischen Effektivität und ihrer ökonomischen Effizienz. Nicht zuletzt wegen ihrer Relevanz bei den EnEV‐Anforderungen werden hier beispielhaft Dachsanierungen untersucht.
Vergleichbarkeit von Energiekennwerten aus Nachweisverfahren in Deutschland, Frankreich und der Schweiz
Authors: Rochard Ulrich 
Published: October 2008

AbstractEs gibt in einigen europäischen Ländern Verordnungen, die die europäische Richtlinie zur Gesamtenergieeffizienz von Gebäuden umsetzen sowie weitere Nachweisverfahren, mit denen über die gesetzlichen Anforderungen hinausgehende Energiestandards nachgewiesen werden können. Sie alle stützen sich bei der wärmetechnischen Beschreibung von Gebäuden im Wesentlichen auf die europäischen Normen EN ISO 13789 und EN ISO 13790. Im Rahmen der Studie HabitEnergie “Erfolgsfaktoren beim Bau von energieeffizienten Wohngebäuden – eine Untersuchung in drei europäischen Ländern” wurde u.a. untersucht, inwieweit die Ergebnisse aus den folgenden Nachweisverfahren miteinander vergleichbar sind: SIA 380/1, MINERGIE®, Réglémentation Thermique 2005 (RT2005), Bâtiment Basse Consommation (BBC), Energieeinsparverordnung (EnEV), Anforderungen des Passivhaus‐Insti‐tuts Darmstadt (PHPP).
Inferring gene expression dynamics from reporter protein levels
Authors: Brown David , Lostroh C. Phoebe 
Published: October 2008

AbstractWe present a mathematical method for inferring the dynamics of gene expression from time series of reporter protein assays and cell populations. We show that estimating temporal expression dynamics from direct visual inspection of reporter protein data is unreliable when the half‐life of the protein is comparable to the time scale of the expression dynamics. Our method is simple and general because it is designed only to reconstruct the pattern of protein synthesis, without assuming any specific regulatory mechanisms. It can be applied to a wide range of cell types, patterns of expression, and reporter systems, and is implemented in publicly available spreadsheets. We show that our method is robust to a several possible types of error, and argue that uncertainty about the decay kinetics of reporter proteins is the limiting factor in reconstructing the temporal pattern of gene expression dynamics from reporter protein assays. With improved estimates of reporter protein decay rates, our approach could allow for detailed reconstruction of gene expression dynamics from commonly used reporter protein systems.
Wine biotechnology in South Africa: Towards a systems approach to wine science
Authors: Moore John P. , Divol Benoit , Young Philip R. , Nieuwoudt Hélène H. , Ramburan Viresh , du Toit Maret , Bauer Florian F. , Vivier Melané A. 
Published: October 2008

AbstractThe wine industry in South Africa is over three centuries old and over the last decade has reemerged as a significant competitor in world wine markets. The Institute for Wine Biotechnology (IWBT) was established in partnership with the Department of Viticulture and Oenology at Stellenbosch University to foster basic fundamental research in the wine sciences leading to applications in the broader wine and grapevine industries. This review focuses on the different research programmes of the Institute (grapevine, yeast and bacteria biotechnology programmes, and chemical‐analytical research), commercialisation activities (SunBio) and new initiatives to integrate the various research disciplines. An important focus of future research is the Wine Science Research Niche Area programme, which connects the different research thrusts of the IWBT and of several research partners in viticulture, oenology, food science and chemistry. This 'Functional Wine‐omics' programme uses a systems biology approach to wine‐related organisms. The data generated within the programme will be integrated with other data sets from viticulture, oenology, analytical chemistry and the sensory sciences through chemometrics and other statistical tools. The aim of the programme is to model aspects of the wine making process, from the vineyard to the finished product.
Microbial responses to solvent and alcohol stress
Authors: Taylor Mark , Tuffin Marla , Burton Stephanie , Eley Kirstin , Cowan Don 
Published: October 2008

AbstractIncreasing fuel prices and doubts over the long‐term availability of oil are currently major global concerns. Such concerns have led to national policies and objectives to develop microbially produced alcohols as fuel additives or substitutes. However, in South Africa this solution poses the further dilemma of sourcing a suitable fermentative carbohydrate that will not impact negatively on the availability of staple foods. The solution lies in the use of lignocellulosic materials, currently a waste product of the food and agriculture industries, which could be used in conjunction with a catabolically suitable production strain. In the pursuit of lignocellulosic biofuel production, conventional fermentation strains have been shown to have limited catabolic versatility. However, catabolically versatile engineered strains and novel isolates engineered with ethanologenic pathways have subsequently been shown to exhibit limitations in solvent tolerance, hindering their full potential as economically viable production strains. A considerable volume of research has been reported on the general cellular mechanisms and physiological responses to solvent shock as well as adaptive changes responsible for solvent tolerant phenotypes in mutant progeny. Here we review a number of the more common cell responses to solvents with particular focus on alcohol tolerance.
Melanoma vaccine candidates from chimeric hepatitis B core virus‐like particles carrying a tumor‐associated MAGE‐3 epitope
Authors: Kazaks Andris , Balmaks Reinis , Voronkova Tatyana , Ose Velta , Pumpens Paul 
Published: October 2008

AbstractVaccination of melanoma patients with tumor‐specific antigens recognized by cytotoxic T lymphocytes (CTLs) may produce significant tumor regressions. Here, we suggest a novel type of tumor vaccines, with well‐studied CTL epitopes presented on highly immunogenic virus‐like particle (VLP) carriers. Cancer‐germline gene MAGE‐3 encodes for an antigenic nonapeptide (MAGE‐3168–176 peptide) that is recognized by CTLs on human leukocyte antigen (HLA)‐A1 and HLA‐B35 molecules. A set of recombinant genes encoding hepatitis B virus core protein carrying MAGE‐3 epitope was constructed and expressed in Escherichia coli cells. Variants that led to formation of chimeric VLPs in vivo were purified and analyzed for their DNA binding properties in vitro. VLPs exhibiting the most pronounced nucleic acid binding affinity were selected and loaded either with single‐stranded DNA oligodeoxynucleotides rich in nonmethylated CG motifs, or with longer double‐stranded DNA fragments. Packaged DNA was protected, at least partially, against the action of bacterial DNase. Such highly purified chimeric VLPs with entrapped immunomodulatory sequences could possibly be used as antitumor vaccines.
RIC‐3 and nicotinic acetylcholine receptors: Biogenesis, properties, and diversity
Authors: Treinin Millet 
Published: October 2008

AbstractNicotinic acetylcholine receptors (nAChRs) belong to a diverse and widely expressed family of ion channels. These receptors are pentamers assembled from multiple combinations of subunits, with different subunit compositions producing receptors having different properties and functions. The diverse functions of nAChRs include an essential role in excitation of skeletal muscles and many modulatory roles throughout the central nervous system. Nicotinic receptors are also implicated in a number of brain pathologies such as epilepsy, schizophrenia, and Alzheimer's disease. Thus, it is important to understand the cellular mechanisms controlling both the numbers and the properties of surface expressed nAChRs. Genetic analysis in Caenorhabditis elegans identified a number of proteins specifically needed for biogenesis of nAChRs. Among these proteins is RIC‐3, a member of a family of proteins having conserved structure and function. RIC‐3 influences both surface expression and properties of nAChRs and its effects are subtype specific. Here we suggest that receptor‐specific chaperones such as RIC‐3 may play important roles in controlling receptor diversity by selectively regulating surface expression of nAChRs having specific subunit compositions.
Exploring Chinese consumer repurchasing intention for services: An empirical investigation
Authors: Dongjin LI , Shenghui AN , Kai YANG 
Published: October 2008

Abstract

With the increasingly tough competition, companies are trying to keep existing customers as well as expand the market base in China, which implies that understanding the Chinese consumer repurchasing intention has become one of the important issues. Consumer repurchase intention is an important indicator to predict repurchase behavior. The aim of this paper is to understand the factors that influence consumer repurchase intention in China. Specifically, this paper explores the relationship between perceived values, customer satisfaction, switching costs, purchase interval, and repurchase intention in China with Structural Equation Modeling. The empirical study finds that perceived value, customer satisfaction, and switching costs are positively related to the repurchase intention while purchase interval does not have significant influence on the repurchase intention. In addition, the perceived value is positively related to the customer satisfaction and there is no significant relationship between switching costs and customer satisfaction.

Copyright © 2008 John Wiley & Sons, Ltd.
Controversial product advertising in China: perceptions of three generational cohorts
Authors: Fam Kim‐Shyan , Waller David S. , Ong Fon‐Sim , Yang Zhilin 
Published: October 2008

Abstract

China is a country that has undergone a wide range of significant changes over the last 30 years, economically, politically, and socially. Major events not only have an important effect on the developmental history of a country such as China, but also create a new generational cohort, which can adopt different views and attitudes than those characterizing previous generations. This study analyses the results of a survey of three different generational groups in China, focusing on their attitudes towards the promotion of controversial products and advertising execution techniques. Research results show significant differences between the younger and older generations, especially in regard to gender‐related products and certain advertising execution techniques.

Copyright © 2008 John Wiley & Sons, Ltd.
On the malleable nature of product meaning in China
Authors: Eckhardt Giana M. , Houston Michael J. 
Published: October 2008

AbstractWhile we know that consumers can hold varying meanings for products depending on context, the source of the variation is not clear. We investigate the source of malleable meanings in the Chinese context, as Chinese consumers are especially likely to hold multiple meanings for the same product. We discover that meanings can be inherently malleable, as opposed to one “true” meaning being altered by situational variables, and that the overwhelming importance of interpersonal relationships and the cultural categories that become salient in varying contexts are what allows incongruent meanings to be held non‐problematically in the Chinese context. This suggests that in China, marketing communications can be difficult to craft because consumer interpretations will be contextually driven, and that social relationships should be emphasized.Copyright © 2008 John Wiley & Sons, Ltd.
Saving “face” in China: modernization, parental pressure, and plastic surgery
Authors: Lindridge Andrew M. , Wang Congying 
Published: October 2008

Abstract

Using adolescent women living and working in Shanghai, as our sample group, we argue that a consequence of a modernizing Peoples Republic of China1

Hence forth called China.
are the cultural values embodied in consumption are increasingly representing western consumption narratives. The extreme of this ideal is the consumption of the body through plastic surgery and the construction of an identity reflective of wider societal changes in China. Using an ethno‐consumerist methodology and interviewing women in Shanghai, our findings indicated that participants were encouraged to undertake plastic surgery operations by drawing upon traditional Chinese cultural hierarchies, i.e., family and society, and a need to compete in a modernizing society. Plastic surgery was used by participants then to construct a future biography of themselves as the embodiment of a new China: perfect, successful, and wealthy.

Copyright © 2008 John Wiley & Sons, Ltd.
Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation
Authors: Gao Kun , Chen Younan , Wei Lingling , Li Shengfu , Jin Xi , Cong Cong , Yuan Yu , Long Dan , Li Youping , Cheng Jingqiu , Lu Yanrong 
Published: October 2008

AbstractWe investigated the mechanism underlying the inhibitory effect of rat mesenchymal stem cells (MSCs) on non‐specific mitogen‐stimulated lymphocytes (LCs) and lymphoblasts (LBs). We used MSCs of passages 2–8 prepared from Sprague–Dawley (SD) rats. LCs were isolated from the spleens of SD rats. Mixed LCs reactions of mitomycin C‐treated MSCs with concanavalin A (ConA)‐stimulated LCs or LBs were performed, and the proliferation inhibition effect was tested by MTS assay. The cytotoxicity of MSCs against naïve and ConA‐stimulated LBs was detected, after co‐culturing for 24 h, by lactate dehydrogenase release assay. The rate of apoptosis of ConA‐stimulated LBs was measured by flow cytometry after incubation with MSCs for 9 h in the ratio 10:1. The MSCs were treated with Fas ligand (FasL), transforming growth factor (TGF)‐β, and interleukin (IL)‐10 blocking antibodies and co‐cultured with ConA‐stimulated LBs to observe the apoptosis and growth inhibitory effect. The main outcomes were bone marrow‐derived adherent CD29+, CD44+, CD45−, CD54+, CD95+, and SH‐2+ MSCs. FasL, TGF‐β, and IL‐10 production by MSCs were visualized by immunocytochemical analysis. MSCs exhibited a dose‐dependent growth inhibitory effect on ConA‐stimulated LCs and LBs. When treated with anti‐FasL and anti‐IL‐10 blocking antibodies, the inhibitory effect of MSCs on LBs proliferation, and the effect of apoptosis induction on LBs decreased. Anti‐TGF‐β blocking antibody treatment did not significantly influence MSCs. Therefore, the inhibitory effects of MSCs against activated LBs were significantly stronger than that against naïve LCs. FasL and IL‐10, rather than TGF‐β, play important roles in the immunosuppressive effects of MSCs. Copyright © 2008 John Wiley & Sons, Ltd.
Transcatheter occlusion of a classical BT shunt with the Amplatzer Duct Occluder II
Authors: Kenny Damien , Walsh Kevin P. 
Published: October 2008

AbstractWe report on a 57‐year‐old woman who underwent transcatheter occlusion of a residual classical Blalock‐Taussig shunt with the Amplatzer® Duct Occluder II (ADO II) 35 years following definitive surgical correction for tetralogy of Fallot. © 2008 Wiley‐Liss, Inc.
Retrograde chronic total occlusion intervention: Tips and tricks
Authors: Wu Eugene B. , Chan Wilson W.M. , Yu Cheuk Man 
Published: October 2008

AbstractRetrograde approach via collateral channels in coronary angioplasty for chronic total occlusion (CTO) can improve the success rate. Most interventionists will meet a few cases where the retrograde approach will provide unequaled advantages, but many are held back from taking retrograde approach by lack of proper equipment and expertise. In this article, we give detail description of techniques to shorten the guiding catheter, to traverse the collateral channels, and to cross the CTO. We also illustrate the difficulties in collateral channel crossing with different examples providing a basic guide for case selection purposes. We hope that many others would find rewardingly successful cases of retrograde approach CTO percutaneous coronary intervention, as in our experience. © 2008 Wiley‐Liss, Inc.
Carotid endarterectomy with simultaneous retrograde common carotid artery stenting: Technical Considerations
Authors: Bazan Hernan A. , Sheahan Malachi , Dardik Alan 
Published: October 2008

AbstractCarotid endarterectomy with simultaneous retrograde common carotid artery stenting (CEA‐RCCAS) is performed with increasing frequency to treat tandem common and internal carotid artery stenoses. Technical details are not clearly delineated in the literature. Our procedure aims to maximize procedural ease and cerebral protection. Although the need for the endovascular component being performed first, followed by shunt placement, and the use of short wires and sheaths has been advocated, we describe the avoidance of shunt placement and the use of long sheaths to facilitate the procedure using local anesthesia and cervical blockade. Use of local anesthesia, avoidance of a shunt, and use of a long sheath may increase the procedural applicability and safety in some patients. CEA‐RCCAS permits safe simultaneous treatment of tandem common and internal carotid artery stenoses. The use of technical adjuncts described here will permit further expansion of the procedure to allow additional patients to be treated in this hybrid fashion. © 2008 Wiley‐Liss, Inc.
Is there a preferable DES in diabetic patients? A critical appraisal of the evidence
Authors: Byrne Robert A. , Kastrati Adnan 
Published: October 2008

AbstractDrug‐eluting stent (DES) therapy reduces restenosis in patients with diabetes when compared with bare metal stent implantation. There are significant differences between commercially available DES platforms both in terms of design characteristics and clinical outcomes. Randomized active‐comparator inter‐DES trials powered for clinical endpoints are unlikely to be performed in patients with diabetes, however, direct comparison randomized trials utilizing surrogate endpoints support a superior anti‐restenotic efficacy with sirolimus‐ versus paclitaxel‐eluting stents. Thrombotic stent occlusion may be higher in patients with diabetes compared with nondiabetic patients, though there is no clear signal of a safety differential between the two platforms. Insufficient data on comparative performance in diabetics exist in relation to the approved zotarolimus‐eluting and everolimus‐eluting stent platforms. If all other factors are equal, then there seems to be no reason why the diabetic patient should not receive treatment with the sirolimus‐eluting stent, which appears to have superior antirestenotic efficacy in this patient group. © 2008 Wiley‐Liss, Inc.
Stent fractures in congenital heart disease
Authors: Breinholt John P. , Nugent Alan W. , Law Mark A. , Justino Henri , Mullins Charles E. , Ing Frank F. 
Published: October 2008

AbstractObjective:To describe incidence, characteristics, predictive factors, and sequelae of stent fractures in congenital heart disease.Background:Stent fracture in congenital heart disease patients is rarely reported.Methods:Patients with stents implanted from 1990 to 2006, with subsequent fluoroscopy were enrolled. Information obtained included: stent type, location, balloon diameter, and residual narrowing. Fracture characteristics, radiographic appearance, and clinical sequelae were also obtained in those with fractures identified.Results:Two hundred and sixty‐five patients (583 stents) had fluoroscopy 4.2 ± 3.3 years after stent implantation. The majority of stents (395, 67.7%) were placed in a pulmonary artery (PA) or its branches. Sixteen stents (2.7%) were placed in a right ventricle to pulmonary artery (RV‐PA) conduit. Fourteen fractures (2.4%) were identified 5.0 ± 3.2 years after implantation: RV‐PA conduit 4/16, proximal right PA 4/135, proximal left PA 6/184 (P = 0.003). Identification was primarily made on lateral fluoroscopy. Review of chest radiographs demonstrated fractures in only 71%. In fractured PA stents, five were redilated and three had additional stents placed. Of the four conduit stent fractures: one underwent redilation, one had embolized segments, and one was reinforced with a second stent that also fractured and embolized a segment during redilation at a later catheterization. All fractures were asymptomatic.Conclusions:With the exception of RV‐PA conduit stents, fracture is rare and asymptomatic. PA stent fractures occurred in 2.5% and did not embolize, whereas RV‐PA conduit stent fractures were significantly more common and may embolize. © 2008 Wiley‐Liss, Inc.
A novel technique using a steerable guide catheter to successfully deliver an Amplatzer septal occluder to close an atrial septal defect
Authors: Nounou Majed , Harrison Alex , Kern Morton 
Published: October 2008

AbstractCorrect positioning of an Amplatzer ASD occluder can be difficult in vertical hearts or in those with diminutive or missing septal rims. In this report we describe the use of a steerable sheath to position the device in the plane of the ASD to achieve success. © 2008 Wiley‐Liss, Inc.
Transcatheter occlusion of antegrade pulmonary flow in children after cavopulmonary anastomosis
Authors: Torres Alejandro , Gray Robert , Pass Robert H. 
Published: October 2008

AbstractObjective:To report our experience with transcatheter occlusion of antegrade pulmonary blood flow (APF) for postoperative complications of cavopulmonary anastomosis (BCPA).Background:It has been suggested that limited APF enhances pulmonary arterial growth in patients undergoing BCPA. However, APF may result in suboptimal postoperative hemodynamics and sequelae such as SVC syndrome or prolonged chest tube drainage. For this subgroup, closure of APF may alleviate these problems.Methods:All BCPA procedures where APF was left open from 1995–2005 were reviewed. Symptomatic patients with APF who underwent a cardiac catheterization in the postoperative period comprised the study cohort.Results:179 BCPA procedures were performed during the study period. APF was left patent in 29/179. 6/29 patients (age 10–28 months, median 14 months) presented 12 to 130 day; (median 31 days) with persistent pleural effusions (5) or SVC syndrome (1, Five had a history of a previous pulmonary arterial band (PAB) and one pulmonary stenosis. PA pressure was elevated in all (range 17–27 mmHg; median 22 mmHg). Hemodynamic evaluation with temporary APF occlusion was repeated in all patients. APF was successfully closed in 4/6 patients. The Amplatzer POA occluder was used in 3 and the Amplatzer ASD occluder in 1. Pulmonary effusions resolved in all the patients who had transcatheter APF closure as did the case of SVC syndrome. There were no complications.Conclusion:Transcatheter APF occlusion seems both safe and feasible in patients with hemodynamic compromise following BCPA with residual APF. Temporary occlusion testing prior to permanent device closure is recommended. © 2008 Wiley‐Liss, Inc.