bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rural‐specific identity and associations with lifestyle behaviors and well‐being among rural cancer survivors
Authors: Tatum Kristina L. , Morris Bonny B. , Glasgow Trevin E. , Lee Sam Mool (Julie) , Barsell D. Jeremy , Fugate‐Laus Kendall , Fuemmeler Bernard F. 
Published: April 2024

AbstractBackgroundDisparities in rural cancer survivors’ health outcomes are well‐documented, yet the role of sociocultural aspects of rurality, such as rural identity, attitudes toward rurality, and social standing on health beliefs and behaviors remain unclear. This study aimed to address these gaps.MethodsRural cancer survivors (N = 188) completed a mailed/online survey. Regression analyses identified relationships among rural identity, negative attitudes toward rurality, and social standing with health outcomes, quality of life, cancer fatalism, and cancer information overload.ResultsHigher rural identity was associated with believing everything causes cancer (OR = 1.58, p = 0.048), believing “there's not much you can do to lower your chances of getting cancer” (OR = 2.22, p = 0.002), and higher odds of being overloaded with cancer information (OR = 2.05, p  = 0.008). Negative attitudes toward rurality was linked with higher levels of perceived stress (B = 0.83, p = 0.001), and chronic pain (OR = 1.47, p = 0.039). Higher subjective social status was associated with perceived social support (B = 0.09, p = 0.016), better overall health (B = 0.13, p < 0.001), lower levels of perceived stress (B = –0.38, p = 0.007), and chronic pain (OR = 0.80, p = 0.027).ConclusionSociocultural factors of rurality were associated with indicators of quality of life, cancer fatalism, and information overload. Further exploration of the underlying mechanisms that drive these associations can help improve intervention targets for rural cancer survivors.
Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker‐free gene editing in Trypanosoma and Leishmania
Authors: Asencio Corinne , Hervé Perrine , Morand Pauline , Oliveres Quentin , Morel Chloé Alexandra , Prouzet‐Mauleon Valérie , Biran Marc , Monic Sarah , Bonhivers Mélanie , Robinson Derrick Roy , Ouellette Marc , Rivière Loïc , Bringaud Frédéric , Tetaud Emmanuel 
Published: April 2024

AbstractKinetoplastids are unicellular eukaryotic flagellated parasites found in a wide range of hosts within the animal and plant kingdoms. They are known to be responsible in humans for African sleeping sickness (Trypanosoma brucei), Chagas disease (Trypanosoma cruzi), and various forms of leishmaniasis (Leishmania spp.), as well as several animal diseases with important economic impact (African trypanosomes, including Trypanosoma congolense). Understanding the biology of these parasites necessarily implies the ability to manipulate their genomes. In this study, we demonstrate that transfection of a ribonucleoprotein complex, composed of recombinant Streptococcus pyogenes Cas9 (SpCas9) and an in vitro‐synthesized guide RNA, results in rapid and efficient genetic modifications of trypanosomatids, in marker‐free conditions. This approach was successfully developed to inactivate, delete, and mutate candidate genes in various stages of the life cycle of T. brucei and T. congolense, and Leishmania promastigotes. The functionality of SpCas9 in these parasites now provides, to the research community working on these parasites, a rapid and efficient method of genome editing, without requiring plasmid construction and selection by antibiotics but requires only cloning and PCR screening of the clones. Importantly, this approach is adaptable to any wild‐type parasite.
Evaluation of modified clear Twin Block aligner in treating adolescents with skeletal class II malocclusion: A two‐centre cephalometric study
Authors: Feng Qingchen , Jiang Fulin , Wang Huixia , Shen Gang , Zhao Zhihe , Jiang Lingyong , Jiang Chen , Fan Qi , Zhu Jinyi , Pan Peiyue , Huang Wenlong , Li Juan 
Published: April 2024

AbstractIntroductionThe purpose of this study was to evaluate the therapeutic effect of modified clear Twin Block (CTB) aligner and traditional twin block (TB) appliance from skeletal, dentoalveolar and soft tissue changes in adolescents with skeletal class II malocclusion.MethodsA total of 80 adolescents, included in this study from two medical centres, were distributed into CTB group, TB group and control group based on the treatment they received. Lateral cephalograms at pre‐treatment (T1) and post‐treatment (T2) were measured by modified Pancherz's cephalometric analysis, and dentoskeletal and soft tissue changes were analysed by independent‐sample t‐test, paired‐sample t‐test, ANOVA test and Scheffe's Post Hoc test.ResultsSeventy‐five adolescents completed the study, including 32 in the CTB group, 32 in the TB group and 11 in the control group. Both CTB and TB treatment showed significant differences in most dentoskeletal and soft tissue measurements. Compared with the control group, improvements were observed in class II molar relationship through significant different in S Vert/Ms‐S Vert/Mi in the CTB group (P < .01) and the TB group (P < .001), as well as deep overjet through significant different in S Vert/Is‐S Vert/Ii in the CTB group (P < .001) and the TB group (P < .001). Besides, the CTB group also showed less protrusion of lower incisors and resulted in a more significant improvement in profile with fewer adverse effects on speaking, eating and social activities.ConclusionsFor adolescents with skeletal class II malocclusion, CTB appliance was as effective as TB on improving dentoskeletal and soft tissue measurements, featuring more reliable teeth control and patient acceptance.
Effects of short‐term exposure to red or near‐infrared light on axial length in young human subjects
Authors: Swiatczak Barbara , Schaeffel Frank 
Published: April 2024

AbstractPurposeTo determine whether visible light is needed to elicit axial eye shortening by exposure to long wavelength light.MethodsIncoherent narrow‐band red (620 ± 10 nm) or near‐infrared (NIR, 875 ± 30 nm) light was generated by an array of light‐emitting diodes (LEDs) and projected monocularly in 17 myopic and 13 non‐myopic subjects for 10 min. The fellow eye was occluded. Light sources were positioned 50 cm from the eye in a dark room. Axial length (AL) was measured before and after the exposure using low‐coherence interferometry.ResultsNon‐myopic subjects responded to red light with significant eye shortening, while NIR light induced minor axial elongation (−13.3 ± 17.3 μm vs. +6.5 ± 11.6 μm, respectively, p = 0.005). Only 41% of the myopic subjects responded to red light exposure with a decrease in AL and changes were therefore, on average, not significantly different from those observed with NIR light (+0.2 ± 12.1 μm vs. +1.1 ± 11.2 μm, respectively, p = 0.83). Interestingly, there was a significant correlation between refractive error and induced changes in AL after exposure to NIR light in myopic eyes (r(15) = −0.52, p = 0.03) and induced changes in AL after exposure to red light in non‐myopic eyes (r(11) = 0.62, p = 0.02), with more induced axial elongation with increasing refractive error.ConclusionsIncoherent narrow‐band red light at 620 nm induced axial shortening in 77% of non‐myopic and 41% of myopic eyes. NIR light did not induce any significant changes in AL in either refractive group, suggesting that the beneficial effect of red laser light therapy on myopia progression requires visible stimulation and not simply thermal energy.
10 kHz stimulation as rescue therapy for spinal cord stimulation trial failure or loss of efficacy: A retrospective study
Authors: Buonanno Pasquale , Servillo Giuseppe , Visser‐Vandewalle Veerle , Matis Georgios 
Published: April 2024

AbstractIntroductionSpinal cord stimulation (SCS) is currently used for the management of pain of different origin, and since its inception, many waveforms have been developed. Some patients experience no pain relief already during SCS trial, while other patients go through a loss of efficacy due to habituation after a variable period of satisfying pain control. Our retrospective study represents the first report exploring the potential role of 10 kHz stimulation as rescue therapy for patients who did not benefit not only from conventional stimulation but even from other waveforms during SCS trial or follow‐up.MethodsThis study was conducted in Germany; we retrospectively enrolled patients with no pain relief during SCS trial or with loss of efficacy of other waveforms over time; and we recorded visual analogic scale (VAS), Oswestry Disability Index (ODI), and daily opioid consumption expressed as morphine milligram equivalents (MME), right before and 12 months after the switching to 10 kHz simulation.ResultsThe rate of successful switching to 10 kHz stimulation was comparable in patients enrolled during the SCS trial and during the follow‐up (43% vs. 40%, respectively); notably, the highest rate of failed rescue was recorded in case of persistent spinal pain syndrome (PSPS) II. Patients who responded to the switching showed a significant improvement in VAS and ODI after 12 months of treatment compared to baseline (3.6 ± 1.0 vs. 8.2 ± 0.9, p < 0.00001 and 34.0 ± 7.8 vs. 64.3 ± 8.7, p < 0.0001, respectively), whereas there was no reduction in the consumption of opioids in terms of MME (3 (0–16) vs. 5 (0–8.75), p = 0.1003).ConclusionsRescue therapy with 10 kHz stimulation could be an important strategy to avoid SCS explant in both patients non‐responsive during trial or experiencing a loss of efficacy during the years with other waveforms.
Lymphoplasmocytic plaque in children: A report of an atypical location
Authors: Teixeira João , Pereira Sofia , Xará Joana Resende , Cardoso José Carlos , Ramos Leonor 
Published: April 2024

AbstractLymphoplasmocytic plaque in children (LPC) is a rare and distinctive skin disorder primarily affecting the pediatric population. Characterized by its unique histopathological features, the condition manifests as well‐defined plaques with a predominance of lymphocytes and plasma cells infiltrating the dermis. Despite its limited prevalence, recognizing this entity is crucial for accurate diagnosis and appropriate management of affected patients. We report the case of a 10‐year‐old male presenting with LPC in the extensor surface of the upper arm, a rarely reported location, treated with both topical and intralesional corticosteroids resulting in partial improvement.
Hormonal response to recurrent seasonal stress in coastal and mountain scabiouses growing in their natural habitat: linking ABA and jasmonates with photoprotection
Authors: Morales Melanie , Munné‐Bosch Sergi 
Published: April 2024

AbstractPlant species distribution across ecosystems is influenced by multiple environmental factors, and recurrent seasonal stress events can act as natural selection agents for specific plant traits and limit species distribution. For that, studies aiming at understanding how environmental constraints affect adaptive mechanisms of taxonomically closely related species are of great interest. We chose two Scabiosa species inhabiting contrasting environments: the coastal scabious S. atropurpurea, typically coping with hot‐dry summers in a Mediterranean climate, and the mountain scabious S. columbaria facing cold winters in an oceanic climate. A set of functional traits was examined to assess plant performance in these congeneric species from contrasting natural habitats. Both S. atropurpurea and S. columbaria appeared to be perfectly adapted to their environment in terms of adjustments in stomatal closure, CO2 assimilation rate and water use efficiency over the seasons. However, an unexpected dry period during winter followed by the typical Mediterranean hot‐dry summer forced S. atropurpurea plants to deploy a set of photoprotective responses during summer. Aside from reductions in leaf water content and Fv/Fm, photoprotective molecules (carotenoids, α‐tocopherol and anthocyanins) per unit of chlorophyll increased, mostly as a consequence of a severe chlorophyll loss. The profiling of stress‐related hormones (ABA, salicylic acid and jasmonates) revealed associations between ABA and the bioactive jasmonoyl‐isoleucine with the underlying photoprotective response to recurrent seasonal stress in S. atropurpurea. We conclude that jasmonates may be used together with ABA as a functional trait that may, at least in part, help understand plant responses to recurrent seasonal stress in the current frame of global climate change.
Early intergroup coalition: Toddlers attribute fair distributions to Black rather than White distributors
Authors: Geraci Alessandra , Commodari Elena , Perucchini Paola 
Published: April 2024

AbstractRacial concepts emerge in preschool age, and affect children's evaluations of others’ actions. This research investigated whether 2.5‐year‐old and 7‐year‐old children's (N = 160; 100% White) evaluations may be influenced by an initial racial bias when both out‐group and in‐group protagonists were evaluated directly by attributing the responsibility of negative or positive outcomes (i.e., fair or unfair distributions of resources). After seeing each of two familiarization events, displaying fair and unfair distributions performed by a hidden distributor, toddlers were asked to assign positive and negative outcomes to two distributors that were portrayed on drawings (Experiment 1) or photographs (Experiments 2–3) of White or Black faces. Seven‐year‐old children were assessed with the same forced‐choice character task by using photographs (Experiment 4). Toddlers showed a significant tendency to attribute a fair distribution of resources to Black faces only when these were shown by photographs. Conversely, 7‐year‐olds manifested a pro‐White/anti‐Black racial bias. These findings support prior literature on the age‐related development of race prejudice starting not before the three years of life, and shed light on an initial state that is aimed at intergroup coalitions.
Advances in stem cells treatment of diabetic wounds: A bibliometric analysis via CiteSpace
Authors: Ma Ke , Luo Chao , Du Mindong , Wei Qiang , Luo Qianxuan , Zheng Li , Liao Mingde 
Published: April 2024

AbstractDiabetes is a chronic medical condition that may induce complications such as poor wound healing. Stem cell therapies have shown promise in treating diabetic wounds with pre‐clinical and clinical studies. However, little bibliometric analysis has been carried out on stem cells in the treatment of diabetic wounds. In this study, we retrieved relevant papers published from January 1, 2003, to December 31, 2023, from Chinese and English databases. CiteSpace software was used to analyze the authors, institutions, and keywords by standard bibliometric indicators. Our analysis findings indicated that publications on stem cells in the treatment of diabetic wounds kept increasing. The most prolific author was Qian Cai (n = 7) and Mohammad Bayat (n = 16) in Chinese and English databases, respectively. Institutions distribution analysis showed that Chinese institutions conducted most publications, and the most prolific institution was the Chinese People's Liberation Army General Hospital (n = 9) and Shahid Beheshti University of Medical Sciences (n = 17) in Chinese and English databases, respectively. The highest centrality keyword in Chinese and English databases was “wound healing” (0.54) and “in vitro” (0.13), respectively. There were 8 and 11 efficient and convincing keyword clusters produced by a log‐likelihood ratio in the Chinese and English databases, respectively. The strongest burst keyword was “exosome” (strength 3.57) and “endothelial progenitor cells” (strength 7.87) in the Chinese and English databases, respectively. These findings indicated a direction for future therapies and research on stem cells in the treatment of diabetic wounds.
High‐resolution 3‐D scanning electron microscopy (SEM) images of DOTTM polynucleotides (PN): Unique scaffold characteristics and potential applications in biomedicine
Authors: Kim Michael James , Park Hyun‐Jun , Jung Rae‐Jun , Won Chee‐Youb , Ohk Seul‐Ong , Kim Hong‐Taek , Roh Nark‐Kyung , Yi Kyu‐Ho 
Published: April 2024

AbstractIntroductionPolynucleotides (PN) are becoming more prominent in aesthetic medicine. However, the structural characteristics of PN have not been published and PN from different companies may have different structural characteristics. This study aimed to elucidate the structural attributes of DOT™ PN and distinguish differences with polydeoxyribonucleotides (PDRN) using high‐resolution scanning electron microscopy (SEM) imaging.Materials and methodsDOT™ PN was examined using a Quanta 3‐D field emission gun (FEG) Scanning Electron Microscope (SEM). Sample preparation involved cryogenic cooling, cleavage, etching, and metal coating to facilitate high‐resolution imaging. Cryo‐FIB/SEM techniques were employed for in‐depth structural analysis.ResultsPDRN exhibited an amorphous structure without distinct features. In contrast, DOT™ PN displayed well‐defined polyhedral shapes with smooth, uniformly thick walls. These cells were empty, with diameters ranging from 3 to 8 micrometers, forming a seamless tessellation pattern.DiscussionDOT™ PN's distinct geometric tessellation design conforms to the principles of biotensegrity, providing both structural reinforcement and integrity. The presence of delicate partitions and vacant compartments hints at possible uses in the field of pharmaceutical delivery systems. Within the realms of beauty enhancement and regenerative medicine, DOT™ PN's capacity to bolster cell growth and tissue mending could potentially transform approaches to rejuvenation treatments. Its adaptability becomes apparent when considering its contributions to drug administration and surgical procedures.ConclusionThis study unveils the intricate structural scaffold features of DOT™ PN for the first time, setting it apart from PDRN and inspiring innovation in biomedicine and materials science. DOT™ PN's unique attributes open doors to potential applications across healthcare and beyond.
Soluble polyvinylpyrrolidone‐based microneedles loaded with Sanguis draconis and Salvia miltiorrhiza for treatment of diabetic wound healing
Authors: Tang Jiangdong , Xu Jiawei , Xu Jianda , Fan Zhenmin , Ye Xia , Xia Zhongyu , Guo Meifeng 
Published: April 2024

AbstractBackgroundNowadays, diabetic wound healing remains a crucial challenge due to their protracted and uncertain healing process. Traditional Chinese medicine (TCM) has demonstrated the therapeutic value of Sanguis draconis (SD)‐Salvia miltiorrhiza (SMR) Herb Pair in diabetic wound healing. However, new administration modes are urgently needed for their convenient and wide‐ranging applications.ObjectiveWe propose a soluble polyvinylpyrrolidone‐based microneedle patch containing the herbal extracts of SD and SMR (MN‐SD@SMR) for diabetic wound healing.MethodsThe herbal extracts of SD and SMR are purification and concentration via traditional lyophilization. SD endowed MN‐SD@SMR with functions to improve high glycemic blood environment and migration of keratinocyte and fibroblast cells.ResultsSMR in MN‐SD@SMR could improve blood flow velocity and microcirculation in the wound area. The effectiveness of transdermal release and mechanical strengths of MN‐SD@SMR were verified.ConclusionIntegrating the advantages of these purified herbal compositions, we demonstrated that MN‐SD@SMR had a positive healing effect on the wounds in vitro and vivo. These results indicate that soluble polyvinylpyrrolidone‐based microneedle patch containing the herbal extracts of SD and SMR has a promising application value due to their superior capability to promote diabetic wound healing.
Consideration of the diameter of superficial temporal arteries related to filler injections in the temporal region
Authors: Bae Jung‐Hee , Park Soo Yeon , Yi Kyu‐Ho 
Published: April 2024

AbstractBackgroundThe concavity of the temple due to adipose tissue atrophy from aging accentuates the zygomatic arch and lateral orbital rim, leading to an aged appearance. The use of hyaluronic acid filler in the temporal region has gained popularity due to its procedural simplicity and consistent outcomes.ObjectiveTo evaluate the safety of administering hyaluronic acid filler in the temporal region concerning the frontal branch of the superficial temporal artery, which is at risk of injury.MethodsEmpirical observations were conducted on the internal diameter of the frontal branch of the superficial temporal artery, a critical anatomical site for potential injury.ResultsA significant proportion of the artery segments exhibited an internal diameter below 1 mm. Given that the outer diameter of an 18‐gauge cannula is 1.27 mm, this method can be considered a relatively secure approach for enhancing the temporal region.ConclusionThe use of an 18‐gauge cannula for hyaluronic acid filler administration in the temporal region appears to be a safe and effective method, with the potential risk to the frontal branch of the superficial temporal artery being minimal.
Hydradermabrasion through the lens of Line‐Field Confocal Optical Coherence Tomography
Authors: Razi Shazli , Truong Thu Minh , Khan Samavia , Sanabria Bianca , Rao Babar 
Published: April 2024

AbstractBackgroundHydradermabrasion, also known as “HydraFacial,” is an exfoliative cosmetic procedure for skin rejuvenation that has gained popularity. Despite its increasing popularity, clinical studies validating its efficacy with non‐invasive assessment of histological changes to the skin, are scarce. In this study, we used Line‐Field Confocal Optical Coherence Tomography (LC‐OCT), an optical imaging device, to non‐invasively visualize microscopic changes to skin anatomy after hydradermabrasion treatment.Materials/MethodsEight volunteers (Fitzpatrick skin types II–V) were recruited for this study. Images, using LC‐OCT (DeepLive, DAMAE medical) were obtained before and after hydradermabrasion and at 2 weeks post‐treatment. A commercially available hydradermabrasion device was utilized to perform the dermabrasion.ResultsIn the epidermis, initially, a decrease in the average thickness of the stratum corneum, from 9.42 to 6.67 µm was visualized in LC‐OCT images after hydradermabrasion. However, at 2 weeks of follow‐up, the average stratum corneum thickness was 9.75 µm, resulting in an overall increase in the average thickness after treatment. Improved homogenization of the stratum corneum and decreased number of undulations in the epidermis post‐treatment were also visualized. In all the subjects, the superficial dermis appeared stretched, which returned to baseline by the 2‐week follow‐up. At the 2‐week follow‐up, there were no visible differences in the quality and quantity of collagen fibers in the dermis.ConclusionIn our study, LC‐OCT images of the epidermis and dermis demonstrated microscopic features of skin rejuvenation when treated with hydradermabrasion. Thus, not only highlighting the efficacy of hydradermabrasion but also the potential of LC‐OCT to serve as a tool for visualizing the microscopic effects of cosmetic procedures on skin anatomy.