bluuebunny/crossref_metadata_2025_split huggingface dataset:
Missed Opportunities for Preventing or Diagnosing Acute Rheumatic Fever: A Retrospective Cohort Study of 20 Young Australians Diagnosed With Rheumatic Heart Disease on Screening Echocardiography
Authors: Rouhiainen Oliver , Gatti Jessica , Ramadani Seide , Stewart Jayden , Matthews Melanie , Fairhurst Helen , Remenyi Bo , Francis Joshua R. , Yan Jennifer 
Published: February 2025

ABSTRACTBackground and AimA cross‐sectional echocardiographic screening study in a remote Aboriginal community in Australia identified hyperendemic levels of rheumatic heart disease (RHD). 20/613 screened were newly diagnosed with RHD, and 18/20 had no known history of acute rheumatic fever (ARF). Our aim was to explore the medical histories of those newly diagnosed with RHD for potential (1) missed opportunities for primary prevention of ARF through management of Group A Streptococcal (Strep A) infection, and (2) missed opportunities for diagnosis of ARF.MethodsWe assessed community clinic and tertiary hospital medical records of those children with new diagnoses of RHD (n = 20), and collected information regarding previous episodes of possible Strep A infection or its sequelae.ResultsAll 20 participants had previous documented possible or confirmed Strep A infections, predominantly in the form of impetigo or infected scabies (range 1–17 episodes per participant). At least 15/20 participants had potential Strep A infections where treatment that was documented may not have adequately covered Strep A. 11/20 participants had scabies diagnosed without documented scabies treatment. 2/20 participants had previously diagnosed ARF, however these were diagnosed within the month prior to the community‐wide screening for RHD. 2/20 participants had undiagnosed, probable or possible ARF when classified retrospectively from review of their case documentation. 13/20 participants had non‐specific presentations with joint complaints that may have represented an ARF episode but with inadequate workup to fulfil diagnostic criteria on retrospective assessment.
Hepatocytes and Hepatic Stellate Cells Carry Different Levels of DNA Damage due to Their Sensitivity to Oxidative Stress in Chronic Hepatitis B
Authors: Zhang Yansong , Jin Danjing , Zhu Hongqiong , Lin Minyi , Peng Xiaomou 
Published: February 2025

ABSTRACTHepatocellular carcinoma (HCC) and liver cirrhosis (LC) occur in spite of current antiviral therapies in patients with chronic hepatitis (CHB). It is not yet known why HCC and LC are related to hepatocytes and hepatic stellate cells (HSCs), respectively, in the same inflammation circumstances. The expression of the phosphorylated form of histone H2AX (γ‐H2AX), a biomarker of DNA damage, was detected in hepatocytes and interstitial cells within the liver tissues of 69 patients with CHB using immunohistochemical assay and immunofluorescence colocalisation technique. Hydrogen peroxide (H2O2) was applied to establish an oxidative DNA damage model. Hepatocytes in CHB patients carried much higher levels of DNA damage than interstitial cells. The DNA damage‐carried interstitial cells were confirmed to be HSCs. They lost the damaged DNA during differentiation into myofibroblasts near the foci of inflammatory necrosis. Hepatocyte was much more sensitive to oxidative stress and DNA damage than HSCs, but both MIHA and LX‐2 repaired DNA damage efficiently in vitro. Hepatocytes carried much higher levels of DNA damage than HSCs due to their remarkable difference in sensitivity to inflammation‐induced oxidative DNA damage. The different sensitivity may render hepatocytes and HSCs to be respectively involved in HCC and LC in the same inflammation circumstances.
Sintering Mechanisms in Metal Extrusion-based Sintering-assisted Additive Manufacturing: State-of-the-Art and Perspectives
Authors: Liu Yulin , Jiang Dayue , Ning Fuda 
Published: February 2025

Abstract
Extrusion-based sintering-assisted additive manufacturing (ES-AM) has enabled the fabrication of intricate metal structures, spanning from simple geometries to complex lattice structures. The sintering process plays a vital role in metal densification that requires effective design and optimization of sintering processes for high-quality sintered parts. Notably, sintering behaviors in ES-AM differ from those in traditional methods, primarily due to the heterogeneous distribution of particles and pores induced by the anisotropic fabrication nature of AM. This literature review offers an overview of process and mechanisms of sintering that lay the foundation for the development of ES-AM. Theories governing solid-state sintering and liquid-phase sintering are summarized to advance a thorough comprehension of associated sintering mechanisms. Computational studies on sintering processes at different length scales are also discussed, including atomic-level molecular dynamics, micro-level simulations (Monte Carlo, phase field, and discrete element method), and macroscopic continuum models. The distinctive anisotropic sintering behaviors in the ES-AM process are further elucidated across multiple levels. Ultimately, future directions for ES-AM, encompassing materials, sintering process, and sintering mechanisms, are outlined to guide research endeavors in this field. This review summarizes multi-scale sintering behaviors in both traditional manufacturing and AM, contributing to a deeper understanding of sintering mechanisms and paving the way for innovations in the next generation of manufacturing, particularly in AM involving sintering mechanism.
Visual Athlete Rebrands: Discussing the Factors That Influence Athletes to Alter Their Personal Brand Logo
Authors: Pedersen Zack P. , Blaszka Matthew , Walsh Patrick 
Published: February 2025

Even though the widespread practice of athletes utilizing a visual symbol to represent and communicate their brand (i.e., a personal logo) is still in its infancy, a number of athletes who have previously established a personal logo have undergone a visual rebrand. The practice of rebranding is not seen as an enviable branding tactic in other realms (e.g., professional teams and business), given that its utilization is typically tied to a miscommunication of the brand or lacking consumer connection. Furthermore, the consequences of athlete branding may even be amplified in comparison to that of team or organizational brands, given the significantly shorter brand life cycle that athletes have to communicate their associations and logo. However, athletes are still using this brand management strategy, indicating a need to further examine rebranding in this context. This commentary discusses current examples of athlete rebrands, what these rebrands communicate, the influence that endorsers have in this phenomenon, and how future scholars can utilize communication theories to better understand this branding strategy.
Detraining Effect on Sprint Swimming Performance and Load–Velocity Profile
Authors: Ruiz-Navarro Jesús J. , López-Belmonte Óscar , Febles-Castro Adrián , Gay Ana , López-Contreras Gracia , Morales-Ortiz Esther , Arellano Raúl 
Published: February 2025

Purpose: To assess the effect of 5-week training-cessation period on performance and load–velocity profile-related variables. Methods: Twenty-four competitive swimmers (15 male and 9 female: 19.2 [3.7] and 17.3 [2.3] y, 50-m front-crawl 550 [70], and 572 [51] World Aquatics points, respectively) performed a 50-m front-crawl all-out swim, a load–velocity profile, and a pull-up test before and after a 5-week off-season period. Kinematic variables, blood lactate concentration, and rating of perceived exertion were monitored during the load–velocity profile tests. Results: Performance was impaired 1.3% for males (P < .01) and 3.8% for females (P < .01). Neither anthropometric changes (males r2 = .277, females r2 = .218, P > .05) nor the physical activity performed during the off-season (males r2 = .329, females r2 = .094, P > .05) attenuated performance impairments. While males counteracted the stroke-rate decline (P < .05) by increasing stroke length (P < .05) in the majority of the race, females did not, leading to a decline in clean swimming speed (P < .05). The maximum load at zero velocity decreased (P < .05) during the load–velocity profile test. In addition, males showed an increased blood lactate concentration (P < .05), whereas females decreased the maximum velocity at zero load (P < .01) and stroke rate (P < .01). No change in the slope was observed for either sex (P > .05). Conclusion: Following a 5-week off-season period, sprint swimming performance declines (males 0.34 s; females 1.15 s). The load–velocity profile and related variables evidenced deterioration, showing changes in blood lactate concentration, maximum load at zero velocity, average velocity during the third trial, and stroke rate.
Rapid and high-sensitivity screening of pregnancy complications by profiling circulating placental extracellular vesicles
Authors: Palma Carlos , Masud Mostafa Kamal , Guanzon Dominic , Lai Andrew , Razo Melissa , Nakahara Angela , Nair Soumyalekshmi , Salas-Burgos Alexis , Hossain Md Shahriar A. , Carrion Flavio , Duncombe Gregory , McIntyre H. David , Handberg Aase , Longo Sherri , Yamauchi Yusuke , Salomon Carlos 
Published: February 2025

Herein, we developed a specific, rapid sensor to quantify placental extracellular vesicle (EV) protein biomarkers of early pregnancy complications. A distinct tetraspanin CD9 and placental alkaline phosphatase (PLAP) expression pattern was observed via targeted multiple reaction monitoring of EVs from maternal plasma collected before 18 weeks of gestation. A classification model was developed using training and validation patient sets, distinguishing between individuals at high risk of developing complications from those with normal pregnancies, achieving 80% sensitivity, 90% specificity, 89% positive predictive value (PPV), and 82% negative predictive value (NPV). Superparamagnetic nanoflowers that captured target EVs (CD9
+
/PLAP
+
) were used to construct a 4-flex glass strip nanozymatic readout system. The sensor analyzes plasma for EVs, identifying gestational diabetes mellitus risk with a 95% combined sensitivity, 100% specificity, 100% PPV, and 96% NPV. This nanoplatform identifies individuals at risk of developing pregnancy complications with a >90% classification accuracy, exhibiting potential for clinical applications.
Optimizing phage therapy for carbapenem-resistant
Enterobacter cloacae
bacteremia: insights into dose and timing
Authors: Fu Shi-Yong , Chen Xiu-Zhen , Yi Peng-Cheng , Gao Jie , Wang Wei-Xiao , Gu Shuang-Lin , Gao Jing-Han , Liu Du-Xian , Xu Han-Feng , Zeng Yi , Hu Chun-Mei , Zheng Qin , Chen Wei 
Published: February 2025

ABSTRACT

The increase in multidrug-resistant (MDR)
Enterobacter cloacae
complex (ECC) infections, particularly those resistant to carbapenems, underscores the urgent need for alternative therapies. Phage therapy, with its specific bactericidal action, offers a promising solution. However, there remains a shortage of well-characterized ECC-targeting phages, and dosing and timing optimization for ECC-specific phage cocktails is largely unexplored. In this study, we isolated and characterized three novel lytic phages with diverse genome sizes and host ranges. Notably, ФEBU8 demonstrated broad-spectrum activity, lysing both
Enterobacter
species and
Acinetobacter baumannii
. ФECL22 displayed stability across a wide temperature range (4–50°C), pH tolerance (6–10), and a burst size of 19 PFU/cell, with OmpA identified as its receptor. Our formulated phage cocktail, comprising ФEBU8, ФECL22, and ФECL30, effectively rescued mice with
E. cloacae
bacteremia in a dose-dependent manner, with a mid-dose regimen showing particularly strong efficacy. Immediate phage administration achieved full survival, whereas a combined prophylactic and therapeutic regimen (“−24 + 6”) also resulted in 100% survival. These findings highlight the critical roles of dosing and timing in optimizing phage therapy for carbapenem-resistant
Enterobacter
infections, with prophylactic use providing a valuable window for delayed treatment and a promising strategy for combating severe bacterial infections.
Dual RNA-seq reveals the complement protein C3-mediated host-pathogen interaction in the brain abscess caused by
Staphylococcus aureus
Authors: Jin Qiyuan , Zhai Yaxuan , Qiang Rui , Ma Xin , Zhao Chenhao , Zhong Jinqi , Li Jijie , Chen Qi , Han Mingxiao , Du Hong , Cong Qifei , Zhang Haifang 
Published: February 2025

ABSTRACT

This study aimed to elucidate the complement protein C3-mediated host-pathogen interaction in the brain abscess caused by
Staphylococcus aureus
infection. Dual RNA-seq was employed to analyze the transcriptomic differences between C3 deficiency and wild-type mice of
S. aureus-
induced brain abscess model, and then we investigated the potential regulatory pathways of
S. aureus-
host interaction mediated by C3 and
S. aureus
genes associated with the pathogenesis of brain abscess. Finally, C3 deficient-mice and
hla
mutants of
S. aureus
were used to verify the specific pathogen-host interaction. In the
S. aureus-
induced brain abscess mouse model, the transcriptomic analysis revealed significant changes in bacterial virulence factors, such as hemolysin. Based on these data, we predicted a regulatory network formed by genes like
hrcA
and
dnaK
, which represent a possible regulation mechanism of
S. aureus
responding to the host. Furthermore, we identified that
hla
was the C3 response gene in
S. aureus
. From the host perspective, we observed that the absence of C3 significantly impacted the host’s inflammatory response, primarily by altering the gene expression of several key immune and inflammatory pathways. These findings suggest that C3 deficiency may impair the host’s ability to recognize and respond to external pathogens. To the best of our knowledge, this study proposed that
S. aureus
may affect host immune response through C3, and C3 plays a critical role in regulating inflammation and immune signaling pathways in the brain abscess caused by
S. aureus
infection.

IMPORTANCE

In this work, we employed immunofluorescence and Western blot analysis to reveal a significant upregulation of microglia-derived C3 in the brain abscess mice model caused by
S. aureus
infection. By integrating the individual RNA sequencing data of
S. aureus
and the dual RNA-seq data of
S. aureus
infection brain abscess mice model, the potential regulatory pathways between
S. aureus
and host were identified, and host C3 not only affects the immune response but also mediates the regulation network of
S. aureus
. This study provided the potential novel targets for therapeutic strategies in mitigating the effects of
S. aureus
infections and improving treatment outcomes.
Cardiovascular health effects of vaping e-cigarettes: a systematic review and meta-analysis
Authors: Kundu Anasua , Feore Anna , Sanchez Sherald , Abu-Zarour Nada , Sutton Megan , Sachdeva Kyran , Seth Siddharth , Schwartz Robert , Chaiton Michael 
Published: February 2025

BackgroundThere is substantial interest in the cardiovascular effects of e-cigarette use, highlighting the need to update our knowledge on the subject. We conducted this review to analyse whether e-cigarette use increases cardiovascular health risks and how these risks vary among different populations.MethodsWe searched six databases and included peer-reviewed human, animal, cell/in vitro original studies but excluded qualitative studies, which were published between July 2021 and December 2023. Three types of e-cigarette exposure were examined: acute, short-to-medium term and long term. Different risk of bias tools were used for assessing the quality of the included human studies and we conducted meta-analysis when possible.ResultsWe included 63 studies in the main analysis, 12 studies in the meta-analysis and 32 studies in the sociodemographic factor-based subgroup analysis. Over half of the human studies had low risk of bias. Acute exposure to e-cigarette was associated with increased heart rate (HR) (mean difference (MD) 11.329, p<0.01) and blood pressure (BP) (MD 12.856, p<0.01 for systolic; MD 7.676, p<0.01 for diastolic) compared with non-use. While HR was lower after acute exposure to e-cigarettes compared with cigarettes (MD −5.415, p<0.01), no significant difference in systolic or diastolic BP was observed. Non-smoker current vapers had no significant differences in resting HR and BP compared with non-users but lower resting HR (MD −2.608, p<0.01) and diastolic BP (MD −3.226, p<0.01) compared with non-vaper current smokers. Despite some association between e-cigarette and endothelial dysfunction, short-to-medium-term transition from cigarettes to e-cigarettes may improve blood flow and BP, particularly among females and younger individuals. There is lack of evidence supporting any association of e-cigarette use with cardiovascular diseases and cardiac dysfunction or remodelling.ConclusionsThis review highlighted several important cardiovascular impacts of e-cigarette use compared with non-use and cigarette smoking. However, the evidence is still limited and requires future research.PROSPERO registration numberCRD42023385632.
Exploiting Defenses against GAN-Based Feature Inference Attacks in Federated Learning
Authors: Luo Xinjian , Zhang Xianglong 
Published: February 2025

Federated learning (FL) is a decentralized model training framework that aims to merge isolated data islands while maintaining data privacy. However, recent studies have revealed that Generative Adversarial Network (GAN) based attacks can be employed in FL to learn the distribution of private datasets and reconstruct recognizable images. In this paper, we exploit defenses against GAN-based attacks in FL and propose a framework, Anti-GAN, to prevent attackers from learning the real distribution of the victim’s data. The core idea of Anti-GAN is to manipulate the visual features of private training images to make them indistinguishable to human eyes even restored by attackers. Specifically, Anti-GAN projects the private dataset onto a GAN’s generator and combines the generated fake images with the actual images to create the training dataset, which is then used for federated model training. The experimental results demonstrate that Anti-GAN is effective in preventing attackers from learning the distribution of private images while causing minimal harm to the accuracy of the federated model.
State of Health Estimation of Lithium-Ion Batteries with Feature Interpretability Based on Partial Charge Curves
Authors: Cheng Xiaorong , Gao Xiyuan , Ma Feng , Wang Yan 
Published: February 2025

Abstract
Accurately estimating the state of health (SOH) of lithium-ion batteries is crucial for ensuring their safety and reliability. The "black box" nature of neural networks makes it difficult to interpret prediction results, while the prediction accuracy of neural networks relies on the reliability of feature extraction. This study proposes a method that utilizes Layer-wise Relevance Propagation (LRP) to explain the importance of features, weights the features based on their relevance scores, and estimates SOH using the weighted features. Savitzky-Golay smoothing filter is applied to denoise aging feature data, enhancing the feature correlation of the smoothed data. Additionally, an LRP-LSTM model is employed to capture time-series information related to SOH. An interpretable model not only explains features but also provides feedback to the model, improving its generalization ability. The proposed method achieves an average RMSE of 1.345% and 1.347% on two datasets, respectively.
CXCL10 Secreted by Pericytes Mediates TNFα-Induced Vascular Leakage in Tumors and Enhances Extravasation of Nanoparticle-Based Chemotherapeutics.
Authors: Seynhaeve Ann L.B. , Liu Hui , Priester Marjolein I. , Valentijn Mike , van Holten-Neelen Conny , Brouwer Rutger W.W. , van Brakel Mandy , Dik Willem A. , van IJcken Wilfred F.J. , Debets Reno , Stubbs Andrew P. , ten Hagen Timo L. M. 
Published: February 2025

Abstract
Tumor necrosis factor-alpha (TNFα) induces vascular permeability and plays an important role in inflammation. In addition, TNFα-induced vascular leakage is involved in the increased extravasation of nanoparticle-formulated chemotherapeutic drugs, improving drug delivery and subsequent tumor response. Here, we uncovered a positive correlation between the presence of pericytes in the tumor-associated vasculature and TNFα-induced leakage and drug delivery, especially when drugs were encapsulated in nanoparticles. RNA-sequencing and pathway analysis identified high expression of CXCL10 in TNFα-stimulated pericytes. In addition, TNFα increased CXCL10 protein production by pericytes in vitro. In animal studies, tumor types with vessels with high pericyte coverage showed enhanced permeability and extravasation of drugs encapsulated in nanoparticles following treatment with TNFα, which could be blocked with a CXCL10 neutralizing antibody. In contrast, tumors harboring vessels with low pericyte numbers did not display increased drug extravasation in response to TNFα. Lack of pericyte coverage could be compensated by co-administration of CXCL10. These findings reveal a mechanism by which TNFα induces CXCL10 release from pericytes, resulting in increased endothelial permeability, vascular leakage, and drug delivery.
The Cosmic Stain in Jean-Luc Nancy’s Thought of the World
Authors: Sá Cavalcante-Schuback Marcia 
Published: February 2025

Abstract
The article aims to highlight the cosmic dimension of Jean-Luc Nancy’s thoughts on the sense of the world. It argues that his thought of the world is deeply touched by “cosmic stains.” The cosmic dimension is to be understood as “stain” rather than as dimension insofar it is about the moment in which the sentiment that existence is really existing incides upon existence. It is about being touched by the there-is of the there-is itself. The cosmic stain of Nancy’s thoughts of the sense of the world has to do with existence being “exscripted” from ontology when, in the state of the acosmism of the world today, the only thing to hold on to is the existence of existence, which opens a transitive sense of existence and being.
New Rock Art Discoveries in Aṣ Ṣafiyya (Wadi Nafūn, Duqm), Sultanate of Oman
Authors: Fossati Angelo E. , Garba Roman 
Published: February 2025

Abstract
The Nafūn rock art complex, located in al-Wusṭā Governorate in south-central Oman, was discovered in 2020 and has emerged as one of the most extensive rock art sites in southeast Arabia. The site features 59 flat limestone rock surfaces with over 200 South Arabian inscriptions and over 500 engraved figures. These figures include maritime animal depictions such as sea turtles, sperm whales, squid, jellyfish, ray fish, and mola mola, along with more common depictions of camels (sometimes ridden) and horsemen hunting oryx or wild canids. The rock art tradition in the Nafūn area is estimated to reach from the 5th millennium BCE to the 2nd millennium CE, offering a unique window into a way of life that endured for over 6,000 years.