bluuebunny/crossref_metadata_2025_split huggingface dataset:
MCP‐3 (CCL7) delivered by parvovirus MVMp reduces tumorigenicity of mouse melanoma cells through activation of T lymphocytes and NK cells
Authors: Wetzel Kristiane , Struyf Sofie , Van Damme Jo , Kayser Tim , Vecchi Annunciata , Sozzani Silvano , Rommelaere Jean , Cornelis Jan J. , Dinsart Christiane 
Published: December 2006

AbstractMonocyte chemotactic protein 3 (MCP‐3/CCL7), a CC chemokine able to attract and activate a large panel of leukocytes including natural killer cells and T lymphocytes, could be beneficial in antitumor therapy. Vectors were constructed based on the autonomous parvovirus minute virus of mice (MVMp), carrying the human (MCP‐3) cDNA. These vectors were subsequently evaluated in the poorly immunogenic mouse melanoma model B78/H1. The infection of the tumor cells with MCP3‐transducing vector at low virus input multiplicities, but not with wild‐type virus, strongly inhibited tumor growth after implantation in euthymic mice. In a therapeutic B78/H1 model, repeated intratumoral injections of MCP3‐tranducing virus prevented further tumor expansion as long as the treatment was pursued. The antitumor effects of the MCP‐3‐transducing vector were not restricted to this tumor model since they could also be observed in the K1735 melanoma. The depletion of CD4, CD8, NK cells and of interferon γ (IFNγ) in mice implanted with MVMp/MCP3‐infected B78/H1 cells abolished the antitumor activity of the vector. The latter data, together with tumor growth in nude mice and reverse‐transcriptase (RT)‐PCR analyses of MVMp/MCP3‐treated tumors, clearly showed that activated CD4, CD8 and NK cells were indispensable for the antineoplastic effect in the B78/H1 tumor. Altogether, our results show that MCP3‐transducing parvovirus vectors may be quite potent against poorly or nonimmunogenic tumors, even in conditions where only a fraction of the tumor cell population is efficiently infected with recombinant parvoviruses. © 2006 Wiley‐Liss, Inc.
Chondroitin sulfate glycosaminoglycans as major P‐selectin ligands on metastatic breast cancer cell lines
Authors: Monzavi‐Karbassi Behjatolah , Stanley J. Steven , Hennings Leah , Jousheghany Fariba , Artaud Cecile , Shaaf Saeid , Kieber‐Emmons Thomas 
Published: December 2006

AbstractThe metastatic breast cancer cell line, 4T1, abundantly expresses the oligosaccharide sialylated Lewis x (sLex). SLexoligosaccharide on tumor cells can be recognized by E‐ and P‐selectin, contributing to tumor metastatic process. We observed that both selectins reacted with this cell line. However, contrary to the E‐selectin reactivity, which was sLexdependent, P‐selectin reactivity with this cell line was sLex‐independent. The sLex‐Neg variant of the 4T1 cell line with markedly diminished expression of sLexand lack of sLea, provided a unique opportunity to characterize P‐selectin ligands and their contribution to metastasis in the absence of overlapping selectin ligands and E‐selectin binding. We observed that P‐selectin binding was Ca2+‐independent and sulfation‐dependent. We found that P‐selectin reacted primarily with cell surface chondroitin sulfate (CS) proteoglycans, which were abundantly and stably expressed on the surface of the 4T1 cell line. P‐selectin binding to the 4T1 cells was inhibited by heparin and CS glycosaminoglycans (GAGs). Moreover, Heparin administration significantly inhibited experimental lung metastasis. In addition, the data suggest that surface CS GAG chains were involved in P‐selectin mediated adhesion of the 4T1 cells to murine platelets and human umbilical vein endothelial cells. The data suggest that CS GAGs are also the major P‐selectin‐reactive ligands on the surface of human MDA‐MET cells. The results warrant conducting clinical studies on the involvement of cell surface CS chains in breast cancer metastasis and evaluation of various CS types and their biosynthetic pathways as target for development of treatment strategies for antimetastatic therapy of this disease. © 2006 Wiley‐Liss, Inc.
Investigating divergent mechanisms of mesoderm development in arthropods: the expression of Ph‐twist and Ph‐mef2 in Parhyale hawaiensis
Authors: Price Alivia L. , Patel Nipam H. 
Published: December 2006

AbstractThe evolution of mesoderm was important for the development of complex body plans as well as key organ systems. Genetic and molecular studies in the fruitfly, Drosophila melanogaster, have provided the majority of information concerning mesoderm development in arthropods. In Drosophila, twist is necessary for the specification and correct morphogenesis of mesoderm and myocyte enhancing factor 2 (mef2) is involved downstream of twist to activate muscle differentiation. In Drosophila, mesoderm is defined by positional cues in the blastoderm embryo, while in another arthropod group, the amphipod crustaceans, cell lineage plays a greater role in defining the mesoderm. It is not known how different mechanistic strategies such as positional information vs. cell‐lineage‐dependent development affect the timing and use of gene networks. Here we describe the development of the mesoderm in a malacostracan crustacean, Parhyale hawaiensis, and characterize the expression of Parhyale twist and mef2 orthologues. In Parhyale, the mesoderm of the post‐mandibular segments arises mainly through the asymmetric division of mesoteloblasts as the germband elongates. Ph‐twist expression is seen in a subset of segmental mesoderm during germband development, but not during early cleavages when the specific mesodermal cell lineages first arise. ph‐mef2 expression starts after the segmental mesoderm begins to proliferate and persists in developing musculature. While the association of these genes with mesoderm differentiation appears to be conserved across the animal kingdom, the timing of expression and relationship with different mechanisms of mesoderm development may give us greater insight into the ancestral use of these genes during mesoderm differentiation. J. Exp. Zool. (Mol. Dev. Evol.) 310B:24–40, 2008. © 2006 Wiley‐Liss, Inc.
Raman spectroscopic study of the uranyl oxyhydroxide hydrates: becquerelite, billietite, curite, schoepite and vandendriesscheite
Authors: Frost Ray L. , Čejka Jiří , Weier Matt L. 
Published: December 2006

AbstractRaman and infrared spectra of five uranyl oxyhydroxide hydrates, becquerelite, billietite, curite, schoepite and vandendriesscheite, are reported. The observed bands are attributed to the (UO2)2+ stretching and bending vibrations, UOH bending vibrations and H2O and (OH)− stretching, bending and libration modes. The UO bond lengths in uranyls and the OH···O bond lengths are calculated from the wavenumbers assigned to the stretching vibrations. They are close to the values inferred and/or predicted from the X‐ray single‐crystal structure. The complex hydrogen‐bonding network arrangement was proved in the structures of all the minerals studied. This hydrogen bonding contributes to the stability of these uranyl minerals. Copyright © 2006 John Wiley & Sons, Ltd. John Wiley & Sons, Ltd.
Extrusion of banana starch: characterization of the extrudates
Authors: González‐Soto Rosalia A , Mora‐Escobedo Rosalva , Hernández‐Sánchez Humberto , Sánchez‐Rivera Mirna , Bello‐Pérez Luis A 
Published: December 2006

AbstractExtrusion of banana starch was carried out for obtaining extrudates with functional and digestibility characteristics. Experimental design with temperature, screw speed and moisture content as independent variables produced 20 experiments that were studied using response surface methodology to discover the effect of these variables on resistant starch content, water absorption index and water solubility index. The amylose content was 37.4% with high resistant starch (RS) content in the ungelatinized sample, which decreased when banana starch was gelatinized (1.9%). Extrudate samples had a higher RS content than the gelatinized native starch, showing that the extrusion process increased RS content of native starch due to starch depolymerization and during cooling of extrudates those linear chains form an arrangement that cannot be hydrolyzed by α‐amylase. The response surface regression model fitted to experimental results of resistant starch showed good determination coefficient (84%). During model validation for RS, the model explains the experimental results up to 90%. Response surface showed that water absorption index values were high when the temperature, moisture content and screw speed were also high; water solubility values were not affected by screw speed, and both temperature and moisture content had a quadratic effect. Cylindrical structures were observed in powders where RS was present and as RS level increased the cylinders became coarser. Copyright © 2006 Society of Chemical Industry
Rapid analysis of triazolopyrimidine sulfoanilide herbicides in waters and soils by high‐performance liquid chromatography with UV detection using a C18 monolithic column
Authors: Rodríguez‐Delgado Miguel Ángel , Hernández‐Borges Javier 
Published: December 2006

AbstractIn this work, the simultaneous analysis of five triazolopyrimidine sulfoanilide herbicides (flumetsulam, florasulam, metosulam, cloransulam‐methyl, and diclosulam) by HPLC using UV detection and a C18 monolithic column is proposed. The mobile phase which was composed of ACN, water, and formic acid was pumped at a high flow rate (5 mmL/min) providing an analysis time of all the compounds in less than 2.3 min. The LODs were in the low μg/L range (i. e. between 60 μg/L for flumetsulam and 90 μg/L for florasulam) and the calibration curves showed good linearity (R2 > 0.9949). The method was applied to the analysis of these compounds in spiked mineral and tap waters and soils after an SPE preconcentration procedure using C18 cartridges. Mean recovery values ranged between 35 and 110% for water samples providing LODs of the whole procedure in the low ng/L level, down to 280 ng/L, and between 77 and 92% for soil samples with LODs down to 9.38 μg/kg. This is the first time that this family of pesticides is simultaneously analyzed in both types of samples by HPLC and also using a monolithic column.
Lubricating properties of Cyanex 302‐modified MoS2 microspheres in base oil 500SN
Authors: Xiaodong Zhou , Xun Fu , Huaqiang Shi , Zhengshui Hu 
Published: December 2006

AbstractThe lubricating properties of molybdenum disulphide (MoS2) microspheres modified by Cyanex 302 (bis(2,4,4‐trimethylpentyl) monothiophosphinic acid) in base oil 500SN were investigated and compared with those of commercial colloidal MoS2 on a four‐ball tester and an Optimol SRV oscillating friction and wear tester in a ball‐on‐disc contact configuration. The worn surfaces of the flat disc lubricated with the additive‐containing base oil were analysed by means of X‐ray photoelectron spectroscope and scanning electron microscopy. The MoS2 microspheres showed much better extreme pressure properties and anti‐wear and friction‐reduction properties in 500SN than commercial colloidal MoS2. The effective chemical adsorption and protection film formed by the active elements (S and P) and long chain alkyls in Cyanex 302 and boundary‐lubricating transfer film of thin sheets containing the solid lubricant particles (tribochemical products) of the additives could account for the boundary lubrication mechanism. Copyright © 2006 John Wiley & Sons, Ltd.
Wet clutch transmission fluid for AWD differentials: influence of lubricant additives on friction characteristics
Authors: Mäki Rikard , Ganemi Bager , Höglund Erik , Olsson Richard 
Published: December 2006

AbstractIn recent years, several electronically controllable automotive transmission systems using wet clutches as intelligent differentials have emerged on the market. These applications place great demands on the anti‐shudder properties of the transmission fluids used.The aims of this study were (i) to investigate the influence of different additives on the friction characteristics of a transmission fluid for all‐wheel drive systems featuring wet multi‐plate clutch with a sintered brass‐based friction material and, based on this knowledge, (ii) to formulate a new transmission fluid with the desired frictional properties. In addition to excellent anti‐shudder properties, the new fluid was required to lubricate hypoid gears under high load. To meet this requirement, it is necessary to add significant amounts of extreme pressure additives to the base oil, which are known to have an unfavourable influence on anti‐shudder properties, necessitating the adoption of novel additive technologies.The additives studied include anti‐wear additives, friction modifiers, corrosion inhibitors, detergents, antioxidants and extreme pressure additives. This paper shows how different additives affect friction in different ways, and that the interactions between the different additives are important to consider. It was concluded that it is feasible to combine good anti‐shudder properties for wet clutches with good lubrication of hypoid gears. Copyright © 2006 John Wiley and Sons, Ltd.
Modelling of reinforcement corrosion – Corrosion with extensive cathodes
Authors: Warkus J. , Raupach M. 
Published: December 2006

AbstractReinforcement corrosion is the most common reason for the premature deterioration of a concrete structure. In case of a partial depassivation of the reinforcement macrocells are formed with considerable metal removal rates. Thus an assessment of the macrocell current becomes of great concern. To find out, whether this can be achieved by numerical calculations, specimens have been fabricated and simulated by the use of a boundary element program. In this paper the results of the calculations are presented and compared to electrochemical measurements on the real specimens.
Mikrostruktur‐Untersuchungen zum Schwingungsverschleißverhalten von Nickelkomposit‐ und Ni‐P‐Schichten
Authors: Lampke Th. , Leopold A. , Dietrich D. , Podlesak H. , Müller Y. , Wielage B. 
Published: December 2006

AbstractSchwingungsverschleiß führt in vielen Bereichen industrieller Anwendung zu gravierenden Bauteilschäden. Zu deren Vermeidung werden verschiedene Schichten eingesetzt bzw. erprobt, so auch galvanisch abgeschiedene Nickel‐ und Nickelkomposit‐ sowie außenstromlos abgeschiedene Nickel‐Phosphor‐Schichten. Das Verhalten dieser Schichten unter Schwingungsverschleißbeanspruchung wurde charakterisiert. Begleitend erfolgte eine mikrostrukturelle Charakterisierung hinsichtlich Korngröße, Partikelgehalt und Partikelverteilung sowie bezüglich Kristallisation und Ausbildung der tetragonalen Phase Nickelphosphid. Diese Ergebnisse korrelieren gut mit den Martenshärtewerten der Schichten und tragen zum Verständnis des Schwingungsverschleißverhaltens der untersuchten Schichten bei. Wärmebehandelte Ni‐P‐Schichten erreichen die doppelte Härte im Vergleich zu Nickeldispersionsschichten, sie versagen jedoch unter oszillierender Gleitbeanspruchung durch Versprödung, Rissbildung und Enthaftung. Dagegen weisen dispersionshärtende Nanokomposite mit TiO2 ein deutlich günstigeres tribologisches Verhalten auf. Ein Feststoffgehalt von ca. 3 Vol.‐% führt zu Dispersions‐ und Feinkornhärtungseffekten, die einen guten Schutz gegen Schwingungsverschleiß bewirken; die Ni‐Matrix bleibt dabei duktil. Die Inkorporation sehr harter Teilchen (SiC) verstärkt hingegen den Schwingungverschleißprozess durch die abrasive Wirkung der im Tribokontakt befindlichen Partikel.
Alveolar oxygen partial pressure and oxygen depletion rate mapping in rats using 3He ventilation imaging
Authors: Cieślar Katarzyna , Stupar Vasile , Canet‐Soulas Emmanuelle , Gaillard Sophie , Crémillieux Yannick 
Published: December 2006

AbstractA hyperpolarized 3He ventilation imaging protocol was implemented to assess alveolar pO2 values and the oxygen depletion rate in rats. The imaging protocol, which is based on spiral k‐space sampling, was designed to acquire a high signal‐to‐noise ratio (SNR) T1‐weighted ventilation series of images in a single breath‐hold. Simulations were performed to estimate the accuracy and dependence of the pO2 imaging protocol on the image SNR and the RF flip‐angle determination. The imaging protocol was validated in vitro in phantoms and in vivo in rats. Imaging sessions were carried out for different inhaled O2 concentrations ranging from 20% to 40%. Parametric maps of alveolar pO2 and oxygen depletion rate were generated from the series of images. For each investigated animal, the differences in measured alveolar pO2 values are in agreement with the changes in inhaled O2 concentration. The oxygen depletion rates, ranging between 0.7 and 8.0 mbar s−1, are in close agreement with the published values for healthy rats. Magn Reson Med, 2007. © 2006 Wiley‐Liss, Inc.
High frame‐rate simultaneous bilateral breast DCE‐MRI
Authors: Dougherty Lawrence , Isaac Gamaliel , Rosen Mark A. , Nunes Linda W. , Moate Peter J. , Boston Raymond C. , Schnall Mitchell D. , Song Hee Kwon 
Published: December 2006

AbstractA simultaneous bilateral back‐projection method for 3D dynamic contrast‐enhanced (DCE)‐MRI of the breasts was developed and evaluated. Using a double‐side band modulation of the RF slab excitation pulse, discontinuous volumes that included both breasts were simultaneously selected. The number of slice phase‐encoding steps was undersampled by a factor of 2, and the resulting signal aliasing from one volume to the other was removed using SENSE processing. In‐plane encoding was performed with an interleaved radial acquisition reconstructed using dynamic k‐space‐weighted image contrast (KWIC) temporal filtering. Image resolution was 0.5 × 0.5 × 3.0 mm3 with an effective temporal resolution of 15 s for both breast volumes. Combined with the 2× acceleration from SENSE encoding, this is a 16× acceleration factor over a conventional MR bilateral breast scan. An initial evaluation of these methods was performed on a cohort of women who presented with palpable or mammographically visible breast abnormalities. A total of 73 abnormalities were found in 45 of the 54 bilateral examinations that were performed. In 11 of these cases there was a significant finding in the contralateral breast. DCE images of both breasts can be acquired simultaneously, resulting in high‐resolution images as well as rapid sampling of the contrast kinetics. Magn Reson Med 57:220–225, 2007. © 2006 Wiley‐Liss, Inc.
‘Step back in time and live the legend’: experiential marketing and the heritage sector
Authors: Leighton Debra 
Published: December 2006

Abstract

This paper investigates experiential marketing as a potential survival strategy for cultural heritage attractions competing in the leisure and tourism marketplace. The paper explores the nature and scope of the heritage industry, heritage as a consumption‐based experience and the evidence of adoption of the experiential paradigm by a number of sites and attractions. A content analysis (Krippendorf, 1980 cited in Easterby‐Smith et al., 2002) of promotional leaflets and websites and a case study provide the analytical basis for this evaluation and the paper concludes by proposing an indicative framework for experiential marketing in this sector.

Copyright © 2006 John Wiley & Sons, Ltd.
Results of a multicenter retrospective study of a combined medical and surgical approach to pulmonary aspergillosis in pediatric neutropenic patients
Authors: Cesaro Simone , Cecchetto Giovanni , De Corti Federica , Dodero Paolo , Giacchino Mareva , Caviglia Ilaria , Fagioli Franca , Livadiotti Susanna , Salin Federica , Caselli Desireè , Castagnola Elio 
Published: December 2006

AbstractBackgroundInvasive aspergillosis (IA) is a serious problem in patients suffering from hematological malignancies. Surgical resection has been reported to improve disease control and patient survival. There are few reports describing the role of surgery in children with pulmonary IA.ProcedureFrom October 1998 to September 2005, 21 patients fulfilled the inclusion criteria. Demographic and clinical data, as well as type and duration of antifungal therapy; surgery and related complications; time elapsing from surgery to resumption of chemotherapy were collected retrospectively through a specially designed form filled in by each investigator.ResultsEleven males and 10 females, aged between 2 and 17 years underwent one or more surgical lung resections for diagnostic and therapeutic purposes. Surgical complications were reported in three patients. Two patients, who underwent a wedge resection and a lobectomy, respectively, had no fungal lesions detected at surgery. Seventeen of 20 patients with malignancy resumed chemotherapy after a median of 19 days from surgery, range 7–81, and 11 of them underwent hematopoietic stem cell transplantation after a median time of 60 days from surgery, range 19–110. After a median follow‐up of 1.7 years, 12 patients are alive while 9 patients have died from progression of their underlying disease.ConclusionsThis study suggests that the combination of medical antifungal therapy and early surgical excision is a feasible and an effective strategy in pediatric patients with IA. In order to avoid unnecessary surgical procedures, we advise checking the response to antifungal therapy by chest‐computed tomography immediately before the date of surgery. Pediatr Blood Cancer 2007;49:909–913. © 2006 Wiley‐Liss, Inc.
Obstetric and neonatal outcomes in severe fetal ventriculomegaly
Authors: Breeze Andrew C. G. , Alexander Peta M. A. , Murdoch Edile M. , Missfelder‐Lobos Hannah H. , Hackett Gerald A. , Lees Christoph C. 
Published: December 2006

AbstractObjectiveTo determine the early outcome and the incidence of associated structural anomalies in pregnancies complicated by severe fetal ventriculomegaly (VM).MethodsA review of cases of severe fetal VM (posterior horn of lateral ventricle > 15 mm at referral or during prenatal follow‐up) referred to a fetal medicine centre in Eastern England over 4 years from 2001 was made. Results of specialist prenatal investigations including ultrasound (US), karyotype, antiplatelet antibodies and congenital infection screen were noted. Neonatal clinical and cranial US findings, autopsy findings and neurodevelopmental follow‐up at 4 months were obtained.ResultsTwenty cases of severe VM were identified, including 3 with spina bifida. Median gestation at diagnosis was 28 weeks (range 16–36 weeks). Twelve cases had additional intra‐cranial abnormalities and two had abnormalities outside the central nervous system. One case was complicated by toxoplasmosis. There was one case of trisomy 21. Ten pregnancies were terminated. Ten babies were live born, all of whom had VM confirmed, and two of these babies died within 4 months. Of the remaining eight, seven have abnormal neurodevelopment.ConclusionsSevere VM is often diagnosed after the threshold of viability. Termination of pregnancy was requested in about half the cases owing to the risk of long‐term neurodisability, and in all cases diagnosed before 24 weeks. In those live born, there was abnormal outcome in all but one. Copyright © 2006 John Wiley & Sons, Ltd.
Practical considerations in creating school‐wide positive behavior support in public schools
Authors: Handler Marcie W. , Rey Jannette , Connell James , Thier Kimberly , Feinberg Adam , Putnam Robert 
Published: December 2006

AbstractSchool‐wide positive behavior support (SWPBS) has been identified as an effective and efficient method to teach students prosocial skills. It requires both effective behavior support practices and systems that will support these changes, including data‐based decision making among the school leadership team. There are many practical and systemic factors that school personnel should examine before they consider themselves ready for systemic school‐wide changes, including those associated with the (a) leadership team, (b) staff, (c) administration, (d) coach/facilitator, and (e) district. Practical considerations in each of these areas will be identified and discussed so that practitioners can anticipate their needs as they create effective SWPBS, particularly in low performing urban schools. © 2007 Wiley Periodicals, Inc. Psychol Schs 44: 29–39, 2007.