bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unlocking the plant growth-promoting potential of yeast spp.: exploring species from the Moroccan extremophilic environment for enhanced plant growth and sustainable farming
Authors: Raklami Anas , Babalola Olubukola Oluranti , Jemo Martin , Nafis Ahmed 
Published: February 2024

AbstractIn this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their Internal Transcribed spacer (ITS) regions. Our research thoroughly characterizes plant growth-promoting abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa’s growth. Four treatments were employed: (i) control without inoculation (NI), (ii) inoculation with L1, (iii) inoculation with L2, and (iv) inoculation with the mixture L1 + L2. L1 isolated from Toubkal Mountain shared 99.83% sequence similarity to Rhodotorula mucilaginosa. Meanwhile, L2, thriving in the arid Merzouga desert, displayed a similar identity to Naganishia albida (99.84%). Yeast strains were tolerant to NaCl (2 M) and 60% PEG (polyethylene glycol P6000) in case of drought. Both strains could solubilize phsphorus, with L2 additionally demonstrating potassium solubilization. In addition, both strains produce indole acetic acid (up to 135 µl ml−1), have siderophore ability, and produce aminocyclopropane-1-carboxylic acid deaminase. Isolates L1 and L2, and their consortium showed that the single or combined strain inoculation of M. sativa improved plant growth, development, and nutrient assimilation. These findings pave the way for harnessing yeast-based solutions in agricultural practices, contributing to enhanced crop productivity and environmental sustainability.
Young Plasma Rejuvenates Blood DNA Methylation Profile, Extends Mean Lifespan, and Improves Physical Appearance in Old Rats
Authors: Chiavellini Priscila , Lehmann Marianne , Gallardo Maria D , Mallat Martina Canatelli , Pasquini Diana C , Zoller Joseph A , Gordevicius Juozas , Girard Mauricio , Lacunza Ezequiel , Herenu Claudia B , Horvath Steve , Goya Rodolfo G 
Published: February 2024

Abstract
There is converging evidence that young blood conveys cells, vesicles, and molecules able to revitalize function and restore organ integrity in old individuals. We assessed the effects of young plasma on the lifespan, epigenetic age, and healthspan of old female rats. Beginning at 25.6 months of age, a group of 9 rats (group T) was intraperitoneally injected with plasma from young rats until their natural death. A group of 8 control rats of the same age received no treatment (group C). Blood samples were collected every other week. Survival curves showed that from age 26 to 30 months, none of the group T animals died, whereas the survival curve of group C rats began to decline at age 26 months. Blood DNAm age versus chronological age showed that DNAm age in young animals increased faster than chronological age, then slowed down, entering a plateau after 27 months. The DNAm age of the treated rats fell below the DNAm age of controls and, in numerical terms, remained consistently lower until natural death. When rats were grouped according to the similarities in their differential blood DNA methylation profile, samples from the treated and control rats clustered in separate groups. Analysis of promoter differential methylation in genes involved in systemic regulatory activities revealed specific GO term enrichment related to the insulin-like factors pathways as well as to cytokines and chemokines associated with immune and homeostatic functions. We conclude that young plasma therapy may constitute a natural, noninvasive intervention for epigenetic rejuvenation and health enhancement.
Purpose in Life and Its Association to Parkinsonism
Authors: Koga Hayami K , Grodstein Francine , Williams David R , Buchman Aron S , Kubzansky Laura D 
Published: February 2024

Abstract

Objectives
Purpose in life has been associated with diverse health outcomes; however, few studies have examined its associations with progressive motor decline in older adults. We tested if higher purpose would be associated with lower likelihood of incident parkinsonism as well as with lower levels and slower rates of increase in parkinsonian signs.

Methods
Participants were 2,626 older adults from the Rush Memory and Aging Project and Minority Aging Research Study followed for an average of 7.2 years (standard deviation [SD] = 4.6). Purpose was measured using the purpose in life subscale of the modified Ryff’s and Keyes’s measure of psychological well-being. Four parkinsonian signs (i.e., parkinsonian gait, rigidity, bradykinesia, and tremor) were assessed using the United Parkinson’s Disease Rating Scale. We examined purpose with risk of developing incident parkinsonism using Cox proportional hazards models. We also used linear mixed-effect models to assess the association between purpose and parkinsonian sign trajectories.

Results
After including demographics, health conditions, and health behaviors in the model, for a 1-SD increase in purpose, the hazards ratio for incident parkinsonism was 0.88 (95% confidence interval [CI] 0.80, 0.97). A 1-SD increase in purpose was associated with a −0.19 (95% CI −0.24, −0.15) point lower score in the global parkinsonian summary score at baseline but no differences in rate of change were evident.

Discussion
Higher purpose was associated with lower hazards of incident parkinsonism and lower levels of parkinsonian signs at baseline. Associations were seen even after adjustment for a wide range of covariates. Findings suggest higher purpose may contribute to maintenance of healthy physical function among older adults.
Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis
Authors: Peng Yuquan , Cui Lvjun , Wang Ying , Wei Lanxing , Geng Shouyu , Chen Hui , Chen Guoyu , Yang Li , Bie Zhilong 
Published: February 2024

Abstract
Pumpkin CmoNAC1 enhances salt tolerance in grafted cucumbers. However, the potential interactions with other proteins that may co-regulate salt tolerance alongside CmoNAC1 have yet to be explored. In this study, we identified pumpkin CmoDREB2A as a pivotal transcription factor that interacts synergistically with CmoNAC1 in the co-regulation of salt tolerance. Both transcription factors were observed to bind to each other’s promoters, forming a positive regulatory loop of their transcription. Knockout of CmoDREB2A in the root resulted in reduced salt tolerance in grafted cucumbers, whereas overexpression demonstrated the opposite effect. Multiple assays in our study provided evidence of the protein interaction between CmoDREB2A and CmoNAC1. Exploiting this interaction, CmoDREB2A facilitated the binding of CmoNAC1 to the promoters of CmoRBOHD1, CmoNCED6, CmoAKT1;2, and CmoHKT1;1, inducing H2O2 and ABA synthesis and increasing the K+/Na+ ratio in grafted cucumbers under salt stress. Additionally, CmoNAC1 also promoted the binding of CmoDREB2A to CmoHAK5;1/CmoHAK5;2 promoters, further contributing to the K+/Na+ homeostasis. In summary, these findings reveal a crucial mechanism of CmoNAC1 and CmoDREB2A forming a complex enhancing salt tolerance in grafted cucumbers.
A case report of a normal fertile woman with 46,XX/46,XY somatic chimerism reveals a critical role for germ cells in sex determination
Authors: Cheng Dehua , Lu Chang-Fu , Gong Fei , Du Juan , Yuan Shimin , Luo Ke-Li , Tan Yue-Qiu , Lu Guang-Xiu , Lin Ge 
Published: February 2024

Abstract
Individuals with 46,XX/XY chimerism can display a wide range of characteristics, varying from hermaphroditism to complete male or female, and can display sex chromosome chimerism in multiple tissues, including the gonads. The gonadal tissues of females contain both granulosa and germ cells. However, the specific sex chromosome composition of the granulosa and germ cells in 46,XX/XY chimeric female is currently unknown. Here, we reported a 30-year-old woman with secondary infertility who displayed a 46,XX/46,XY chimerism in the peripheral blood. FISH testing revealed varying degrees of XX/XY chimerism in multiple tissues of the female patient. Subsequently, the patient underwent preimplantation genetic testing (PGT) treatment, and 26 oocytes were retrieved. From the twenty-four biopsied mature oocytes, a total of 23 first polar bodies (PBs) and 10 second PBs were obtained. These PBs and two immature metaphase I (MI) oocytes only displayed X chromosome signals with no presence of the Y, suggesting that all oocytes in this chimeric female were of XX germ cell origin. On the other hand, granulosa cells obtained from individual follicles exhibited varied proportions of XX/XY cell types, and six follicles possessed 100% XX or XY granulosa cells. A total of 24 oocytes were successfully fertilized, and 12 developed into blastocysts, where 5 being XY and 5 were XX. Two blastocysts were transferred with one originating from an oocyte aspirated from a follicle containing 100% XY granulosa cells. This resulted in a twin pregnancy. Subsequent prenatal diagnosis confirmed normal male and female karyotypes. Ultimately, healthy boy–girl twins were delivered at full term. In summary, this 46,XX/XY chimerism with XX germ cells presented complete female, suggesting that germ cells may exert a significant influence on the sexual determination of an individual, which provide valuable insights into the intricate processes associated with sexual development and reproduction.
A North-South Agent–Based Model of segmented labor markets: the role of education and trade asymmetries
Authors: Fanti Lucrezia , Pereira Marcelo C , Virgillito Maria Enrica 
Published: February 2024

Abstract
Drawing upon the labour-augmented K+S Agent-Based Model (ABM), this paper develops a two-country North-South ABM wherein the leader and the laggard country interact through the international trade of machines. The model aims to address sources of asymmetries and possible converge patterns between two economies belonging to a currency union, that are initially differentiated only in terms of the education level they are able to ensure. Education is modeled as a macro-level public policy differently targeting three levels, that is primary, secondary and tertiary. After being educated, when workers enter the labour force, they face a segmented labour market, divided into three types of qualifications and resulting functions deployed inside firms, i.e., basic, medium and advanced occupations. The three markets are heterogeneous in terms of both requested education level and minimum offered wage. We experiment with different education and trade settings. Ultimately, we are interested in understanding the coupling effects of asymmetries in education, which reverberate in segmented labour markets and differentiated growth patterns. Notably, our focus on capital-goods trade, rather than on consumption goods, allows us to investigate a direct link between productive capabilities in complex products and country growth prospects.
Biennial patterns in Alaskan sockeye salmon ocean growth are associated with pink salmon abundance in the Gulf of Alaska and the Bering Sea
Authors: Rand Peter S , Ruggerone Gregory T 
Published: February 2024

Abstract
In response to ocean heating and hatchery production, pink salmon (Oncorhynchus gorbuscha) returning from the North Pacific Ocean steadily increased after 1975, leading to concerns about their influence on food webs and competition with other species. Using measurements of distance between scale annuli of 24 584 individual sockeye salmon (O. nerka), we examined growth during their 2 or 3 years at sea from 1977 to 2015 for eight populations in Alaska. We found significant, negative autocorrelations at 1 lag year in annual growth of sockeye salmon, with a consistent pattern of lower growth in odd years, i.e. opposite to the biennial pattern of pink salmon abundance. Peak pink salmon abundances reduced growth of sockeye salmon from 7 to 14% during the second year in the ocean compared with growth when pink salmon abundance was low, while third-year growth was reduced up to 17%. The overall effect of pink salmon abundance on sockeye growth was over two times greater than the effect of sockeye salmon abundance. Production hatcheries and ocean heating contribute to the competitive dominance of pink salmon, underscoring the need to consider this unintended anthropogenic effect on the growth and productivity of sockeye salmon throughout the North Pacific.
Novel para-aortic cardiac assistance using a pre-stretched dielectric elastomer actuator
Authors: Jahren Silje Ekroll , Martinez Thomas , Walter Armando , Clavica Francesco , Heinisch Paul Philipp , Buffle Eric , Luedi Markus Martin , Hörer Jürgen , Obrist Dominik , Carrel Thierry , Civet Yoan , Perriard Yves 
Published: February 2024

Abstract

OBJECTIVES
We propose an evolution of a dielectric elastomer actuator-based cardiac assist device that acts as a counterpulsation system. We introduce a new pre-stretched actuator and implant the device in a graft bypass between the ascending and descending aorta to redirect all blood through the device (ascending aorta clamped). The objective was to evaluate the influence of these changes on the assistance provided to the heart.

METHODS
The novel para-aortic device and the new implantation technique were tested in vivo in 5 pigs. We monitored the pressure and flow in the aorta as well as the pressure–volume characteristics of the left ventricle. Different activation timings were tested to identify the optimal device actuation.

RESULTS
The proposed device helps reducing the end-diastolic pressure in the aorta by up to 13 ± 4.0% as well as the peak systolic pressure by up to 16 ± 3.6%. The early diastolic pressure was also increased up to 10 ± 3.5%. With different activation, we also showed that the device could increase or decrease the stroke volume.

CONCLUSIONS
The new setup and the novel para-aortic device presented here helped improve cardiac assistance compared to previous studies. Moreover, we revealed a new way to assist the heart by actuating the device at different starting time to modify the left ventricular stroke volume and stroke work.
Differential Graded Manifolds of Finite Positive Amplitude
Authors: Behrend Kai , Liao Hsuan-Yi , Xu Ping 
Published: February 2024

Abstract
We prove that dg manifolds of finite positive amplitude, that is, bundles of positively graded curved $L_{\infty }[1]$-algebras, form a category of fibrant objects. As a main step in the proof, we obtain a factorization theorem using path spaces. First we construct an infinite-dimensional factorization of a diagonal morphism using actual path spaces motivated by the AKSZ construction. Then we cut down to finite dimensions using the Fiorenza-Manetti method. The main ingredient in our method is the homotopy transfer theorem for curved $L_{\infty }[1]$-algebras. As an application, we study the derived intersections of manifolds.
Rectifiability of Flat Singular Points for Area-Minimizing mod 2Q Hypercurrents
Authors: Skorobogatova Anna 
Published: February 2024

Abstract
Consider an $ m $-dimensional area minimizing mod$ (2Q) $ current $ T $, with $ Q\in {\mathbb {N}} $, inside a sufficiently regular Riemannian manifold of dimension $ m + 1 $. We show that the set of singular density-$ Q $ points with a flat tangent cone is $ (m-2) $-rectifiable. This complements the thorough structural analysis of the singularities of area-minimizing hypersurfaces modulo $ p $ that has been completed in the series of works of De Lellis–Hirsch–Marchese–Stuvard and De Lellis–Hirsch–Marchese–Stuvard–Spolaor, and the work of Minter–Wickramasekera.
Sustainable deployment of clinical prediction tools—a 360° approach to model maintenance
Authors: Davis Sharon E , Embí Peter J , Matheny Michael E 
Published: February 2024

Abstract

Background
As the enthusiasm for integrating artificial intelligence (AI) into clinical care grows, so has our understanding of the challenges associated with deploying impactful and sustainable clinical AI models. Complex dataset shifts resulting from evolving clinical environments strain the longevity of AI models as predictive accuracy and associated utility deteriorate over time.

Objective
Responsible practice thus necessitates the lifecycle of AI models be extended to include ongoing monitoring and maintenance strategies within health system algorithmovigilance programs. We describe a framework encompassing a 360° continuum of preventive, preemptive, responsive, and reactive approaches to address model monitoring and maintenance from critically different angles.

Discussion
We describe the complementary advantages and limitations of these four approaches and highlight the importance of such a coordinated strategy to help ensure the promise of clinical AI is not short-lived.
Bone and Skin/Subcutaneous Tissue Concentrations of Cefiderocol During Treatment of Extensively Drug-Resistant Pseudomonas aeruginosa
Authors: Mueller Scott W , Molina Kyle C , Blass Brittany , Gibson Cameron , Kohler Amber D , Krsak Martin , Wiktor Arek J 
Published: February 2024

Abstract
Pyoderma gangrenosum is a rare dermatologic disorder that disrupts the skin barrier, requiring immunosuppressive therapy. We successfully used cefiderocol for the treatment of an extensively drug-resistant Pseudomonas aeruginosa bacteremia, and presumed osteomyelitis in a patient with severe pyoderma gangrenosum and associated immunosuppressive therapy while being medically optimized for skin grafting. We obtained bone and skin/subcutaneous tissue while the patient was on cefiderocol under an institutional review board-approved biologic waste recovery protocol. Cefiderocol concentrations in bone and skin/subcutaneous tissue were 13.9 and 35.9 mcg/g, respectively. The patient recovered from bacteremia and underwent autografting without further complications. Cefiderocol at approved dosing of 2 g IV (3-hour infusion) every 8 hours resulted in bone and skin/subcutaneous tissue concentrations adequate to treat extensively drug-resistant Gram-negative bacteria that remain susceptible to cefiderocol.
Exploring the Prevalence of Post-traumatic Stress Disorder and Post-traumatic Stress Symptoms in Parents Within 12 Months of Child Burn Injury: A Systematic Review
Authors: Chouinard Naomi Hope , Ndongo Sonfack Davaine Joel , Chang Sue-Ling , Bergeron Frédéric , Beaudoin Cloutier Chanel , Guertin Jason Robert 
Published: February 2024

Abstract
Our systematic review aimed to investigate the prevalence of post-traumatic stress symptoms (PTSS) and post-traumatic stress disorder (PTSD) among parents within 12 months of their child’s burn injury. A literature search was conducted in PubMed, Embase, Web of Science, Psychinfo, and CINAHL on January 6, 2023, for quantitative studies reporting the prevalence of PTSD and/or PTSS in parents within 12 months following their child’s burn injury. The risk of bias was assessed using the Mixed Methods Appraisal Tool version 2018. A narrative synthesis of prevalence was presented. We identified 15 articles that met our inclusion criteria. The prevalence of PTSS within 12 months following the burn injury ranged from 6% to 49%. Prevalence estimates of PTSD within the 12 months following a burn injury were limited, ranging from 4.4% to 22%. Our findings highlight the significant impact of burn injuries on parental mental health, with a considerable proportion of parents experiencing PTSS within 12 months following their child’s burn injury. Prevalence estimates for PTSD were limited and warrant further investigation. Our review also underscores the need for standardization of PTSS/PTSD terminology. Timely and targeted psychological support is needed for parents in the aftermath of their child’s burn injury.
Best practices on patient education materials in hip surgery based on learnings from major hip centers and societies
Authors: Parsa Ali , Prabhavalkar Omkar N , Saeed Sheema , Nerys-Figueroa Julio , Carbone Andrew , Domb Benjamin G 
Published: February 2024

ABSTRACT
Patient education is important as it gives patients a better understanding of the risks and benefits of medical and surgical interventions. Developing communication technologies have completely changed and enhanced patient access to medical information. The aim of this study was to evaluate available patient education materials (PEMs) regarding hip surgery on the websites of major hip societies and centers. The PEM from 11 selected leading hip centers and societies were evaluated with the following assessment tools: Flesch–Kincaid (FK) readability test, Flesch Reading Ease formula, Literature-Intelligence-Data-Analysis (LIDA) instrument and Discernibility Interpretability Sources Comprehensive Evidence Relevance Noticeable (DISCERN) tool. Videos were assessed using Patient Educational Video Assessment Tool (PEVAT). A total of 69 educational items, including 52 text articles (75.4%) and 17 videos (24.6%) were retrieved and evaluated. The median Interquartile Range (IQR) FK level of 52 text articles was 10.8 (2.2). The median (IQR) LIDA score of text articles by center was 45. According to the LIDA score, 60% of all website articles demonstrated high accessibility (LIDA score &gt; 44). The median DISCERN score of text articles by center was 69. Overall, 52 (100%) of the text articles were deemed to be at ‘good’ quality rating or higher, and 23.2% (16 out of 69) of the articles had excellent quality. The mean PEVAT score for the 17 videos was 25 ± 1.9. Analysis of text and video articles from the 11 leading orthopedic surgery centers and societies demonstrated that by selecting a reliable source of information from main scientific societies and major centers in hip surgery, patients can find more accurate information regarding their hip conditions.
Impact of time from diagnosis to chemotherapy on prognosis in advanced pancreatic cancer
Authors: Nishida Tsutomu , Sugimoto Aya , Hosokawa Kana , Masuda Haruka , Okabe Satoru , Fujii Yoshifumi , Nakamatsu Dai , Matsumoto Kengo , Yamamoto Masashi , Fukui Koji 
Published: February 2024

Abstract

Background
Due to the aggressive nature and poor prognosis of advanced pancreatic cancer, prompt initiation of treatment is critical. We investigated the effect of the interval between cancer diagnosis and initiation of chemotherapy on survival in patients with advanced pancreatic cancer.

Methods
In this retrospective, single-centre study, consecutive patients with advanced pancreatic cancer between April 2013 and March 2022 were analyzed. Data were extracted from the electronic medical records of patients who received chemotherapy for metastatic, locally advanced or resectable pancreatic cancer or who received chemotherapy due to either being intolerant of or declining surgery. We compared overall survival between two groups: the early waiting time group (waiting time ≤30 days from diagnosis to chemotherapy initiation) and the elective waiting time group (waiting time ≥31 days). Prognostic factors, including biliary drainage, were considered. The impact of waiting time on survival was assessed by univariate and multivariate analyses with Cox proportional hazard models. A 1:1 propensity score matching approach was used to balance bias, accounting for significant poor prognosis factors, age and sex.

Results
The study involved 137 patients. Overall survival exhibited no statistically significant difference between the early and elective waiting time groups (207 and 261 days, P = 0.2518). Univariate and multivariate analyses identified poor performance status and metastasis presence as predictors of worse prognosis. This finding persisted post propensity score matching (275 and 222 days, P = 0.8223).

Conclusions
Our study revealed that initiating chemotherapy ˃30 days later does not significantly affect treatment efficacy compared to within 30 days of diagnosis.