bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of platelet aggregation by olive oil phenols via cAMP-phosphodiesterase
Authors: Dell'Agli Mario , Maschi Omar , Galli Germana V. , Fagnani Rossana , Dal Cero Esther , Caruso Donatella , Bosisio Enrica 
Published: October 2007

The aim of the present study was to confirm that olive oil phenols reduce human platelet aggregability and to verify the hypothesis that cAMP- and cGMP- phosphodiesterases (PDE) could be one of the targets of the biological effect. Four extracts from oils characterized by a high phenol content (HPE), and low phenol levels (LPE) were prepared and analyzed quali- and quantitatively by HPLC-UV and electrospray ionization–MS/MS. Human washed platelets stimulated with thrombin were used for the aggregation assay. Human platelet cAMP-PDE and recombinant PDE5A1 were used as enzyme source. Platelet aggregation and enzyme activity were assayed in the presence of HPE, LPE and individual phenols. The phenol content of HPE ranged between 250 and 500 mg/kg, whereas the LPE content was 46 mg/kg. The compounds identified were hydroxytyrosol (HT), tyrosol (TY), oleuropein aglycone (OleA) and the flavonoids quercetin (QU), luteolin (LU) and apigenin (AP). OleA was the most abundant phenol (range 23·3 to 37·7 %) and LU was the most abundant flavonoid in the extracts. Oil extracts inhibited platelet aggregation with an 50% inhibitory concentration interval of 1·23–11·2 μg/ml. The inhibitory effect of individual compounds (10 μm) including homovanillyl alcohol (HVA) followed this order: OleA>LU>HT = TY = QU = HVA, while AP was inactive. All the extracts inhibited cAMP-PDE, while no significant inhibition of PDE5A1 (50μg/ml) was observed. All the flavonoids and OleA inhibited cAMP-PDE, whereas HT, TY, HVA (100 μm) were inactive. Olive oil extracts and part of its phenolic constituents inhibit platelet aggregation; cAMP-PDE inhibition is one mechanism through which olive oil phenols inhibit platelet aggregation.
Natural history of suicidal behaviors in a population-based sample of young adults
Authors: BREZO JELENA , PARIS JOEL , BARKER EDWARD DYLAN , TREMBLAY RICHARD , VITARO FRANK , ZOCCOLILLO MARK , HÉBERT MARTINE , TURECKI GUSTAVO 
Published: October 2007

ABSTRACTBackgroundSuicidal behaviors in young individuals represent an important public health problem. Understanding their natural history and relationships would therefore be of clinical and research value. In this study, we examined the natural histories of several suicidal behaviors and investigated two conceptual models of suicidality (dimensional and categorical) in the context of adolescent and adult-onset suicide attempts.MethodParticipants were members of a prospectively studied, representative, population-based school cohort followed since age 6 (n=3017) through mid-adolescence (n=1715) to their early twenties (n=1684). Outcome measures included suicidal ideation, attempts and completions.ResultsApproximately one in 500 individuals died by suicide. About 33% had suicidal ideas and 9·3% made at least one suicide attempt. Over half (4·9%) of the self-reported attempters made their first attempt before age 18. With the exception of current suicidal ideas, non-fatal suicidal behaviors were more prevalent in females. In general, parental and cross-sectional self-reports underestimated suicidality rates. Aikaike (AIC) and Bayesian (BIC) information criteria suggested the ordinal model, and dimensional conceptualization of suicide attempts of different onset age, to be more optimal than its multinomial/categorical counterpart (ordinal: AIC 567.55, BIC 635.67; multinomial: AIC 616.59, BIC 723.83). Both models, nevertheless, identified five common factors of relevance to suicidal diathesis: gender, disruptive disorders, childhood anxiousness and abuse, and suicidal thoughts.ConclusionsNon-fatal suicidal behaviors in adolescents and young adults are more common than suggested by cross-sectional studies and parental reports. The dimensional model may be more useful in explaining the relationship of suicide attempts of different age of onset.
The contribution of gene–environment interaction to psychopathology
Authors: Thapar Anita , Harold Gordon , Rice Frances , Langley Kate , O'Donovan Michael 
Published: October 2007

AbstractThe study of gene–environment interaction (G × E) constitutes an area of significant social and clinical significance. Different types of research study designs are being used to investigate the contribution of G × E to psychopathology, although the term G × E has also been used and interpreted in different ways. Despite mixed evidence that G × E contributes to psychopathology, some promising and consistent findings are emerging. Evidence is reviewed in relation to depression, antisocial behavior, schizophrenia, and attention-deficit/hyperactivity disorder. Although findings from various research designs have different meaning, interestingly much of the evidence with regard to the contribution of G × E that has arisen from twin and adoption studies has been for antisocial behavior and depression. It is for these same forms of psychopathology that molecular genetic evidence of G × E has also been most convincing. Finally, current and anticipated methodological challenges and implications for future research in this area are considered.
Genetic expression outside the skin: Clues to mechanisms of Genotype × Environment interaction
Authors: Reiss David , Leve Leslie D. 
Published: October 2007

AbstractThe rapidly moving study of Gene × Environment interaction (G × E) needs interim conceptual tools to track progress, integrate findings, and apply this knowledge to preventive intervention. We define two closely related concepts: the social mediation of the expression of genetic influences and the interaction between the entire genotype and the social environment (G × E). G × E, the primary focus of this report, assesses individual differences in the full genotype using twin, sibling, and adoption designs and, for the most part, employs fine-grained analyses of relational processes in the social environment. In comparison, studies of Allele × Environment interaction assess the influence on development of one or more measured polymorphisms as modified by environmental factors. G × E studies build on work showing how the social environment responds to genetic influences and how genetic influences shape the social environment. Recent G × E research has yielded new insight into variations in the sensitivity of the social environment to genotypic influences and provides clues to the specificity and timing of these environmental responses that can be leveraged to inform preventive interventions aimed at reducing genetic risk for problem behavior.
Gene × Environment interactions in speech sound disorder predict language and preliteracy outcomes
Authors: McGrath Lauren M. , Pennington Bruce F. , Willcutt Erik G. , Boada Richard , Shriberg Lawrence D. , Smith Shelley D. 
Published: October 2007

AbstractFew studies have investigated the role of gene × environment interactions (G × E) in speech, language, and literacy disorders. Currently, there are two theoretical models, thediathesis–stress modeland thebioecological model, that make opposite predictions about the expected direction of G × E, because environmental risk factors may either strengthen or weaken the effect of genes on phenotypes. The purpose of the current study was to test for G × E at two speech sound disorder and reading disability linkage peaks using a sib-pair linkage design and continuous measures of socioeconomic status, home language/literacy environment, and number of ear infections. The interactions were tested using composite speech, language, and preliteracy phenotypes and previously identified linkage peaks on 6p22 and 15q21. Results showed five G × E at both the 6p22 and 15q21 locations across several phenotypes and environmental measures. Four of the five interactions were consistent with the bioecological model of G × E. Each of these four interactions involved environmental measures of the home language/literacy environment. The only interaction that was consistent with the diathesis–stress model was one involving the number of ear infections as the environmental risk variable. The direction of these interactions and possible interpretations are explored in the discussion.
Exploring interactive effects of genes and environments in etiology of individual differences in reading comprehension
Authors: Grigorenko Elena L. , Deyoung Colin G. , Getchell Marya , Haeffel Gerald J. , Klinteberg Britt A.F. , Koposov Roman A. , Oreland Lars , Pakstis Andrew J. , Ruchkin Vladislav V. , Yrigollen Carolyn M. 
Published: October 2007

AbstractIt is established that reading and reading-related processes are heritable; genes thus play an important role in the foundation of individual differences in reading. In this article, we focus on one facet of reading–comprehension. Comprehension is a higher order cognitive skill that requires many other cognitive processes for it to unfold completely and successfully. One such process is executive functioning, which has been associated with genetic variation in the catechol-O-methyltransferase (COMT) gene. Genotypes and haplotypes of four single nucleotide polymorphisms in COMT were investigated in 179 incarcerated adolescent delinquents. Four hierarchical logistic regression models predicting the presence/absence of comprehension difficulties were fitted to the data; genetic variation in COMT and the presence/absence of maternal rejection were investigated as main effects and as effects acting interactively. Three out of four interaction terms were found to be important predictors of individual differences in comprehension. These findings were supported by the results of the haplotype analyses, in which the four investigated polymorphisms were considered simultaneously.
Evidence for a gene–gene interaction in predicting children's behavior problems: Association of serotonin transporter short and dopamine receptor D4 long genotypes with internalizing and externalizing behaviors in typically developing 7-year-olds
Authors: Schmidt Louis A. , Fox Nathan A. , Hamer Dean H. 
Published: October 2007

AbstractRecent work on the molecular genetics of complex traits in typical and atypical human development has focused primarilyon associations of single genes with behavior. Disparate literature suggests that the presence of one or two copies of the short allele of the serotonin transporter (5-HTT) gene and the long allele (7-repeat allele) version of the dopamine receptor D4 (DRD4) gene predicts internalizing- and externalizing-related behaviors, respectively. Apparently for the first time in the extant literature, we report a gene–gene statistical interaction on behavior problems in a group of typically developing children at age 7. DNA was extracted from buccal cells collected from 108 children and genotyped for short and long alleles of the5-HTTgene and the short (2–5 repeats) versus long (6–8 repeats) allele of theDRD4gene. Mothers completed the Child Behavior Checklist. As predicted, children with one or two copies of the short allele of the5-HTTgeneandthe long allele version of theDRD4gene exhibited significantly more internalizing and externalizing behaviors at age 7 than children with other combinations of the5-HTTandDRD4short and long genotypes. As well, children with the5-HTTlongand DRD4long genotypes had the lowest reported scores on internalizing and externalizing behaviors at age 7, suggesting that the presence of the5-HTTlong genotype may serve as a protective factor against these behaviors in children with the longDRD4genotype. Implications of these findings for understanding cumulative biological risk and protective factors in childhood behavior problems and psychopathology are discussed.
Assessing gene–environment interactions on anxiety symptom subtypes across childhood and adolescence
Authors: Lau Jennifer Y. F. , Gregory Alice M. , Goldwin Michelle A. , Pine Daniel S. , Eley Thalia C. 
Published: October 2007

AbstractConsistent evidence shows both genetic and stress-related risks on child and adolescent anxiety, yet few studies have considered the degree to which genetic effects are moderated by stress (gene–environment interaction). We used longitudinal data from both a child and adolescent sample of twins to examine three novel issues on the presence of gene–environment interaction on anxiety symptoms. First, we assessed moderation of genetic risks on anxiety symptoms by negative life events in each age group. Second, by distinguishing between “stable” and “age-specific” genetic factors, we explored the continuity of gene–environment interaction across time and/or its emergence at specific ages. Third, we compared the presence of gene–environment interaction across different symptom types (general, panic, social, and separation). Genetic effects on separation anxiety symptoms in childhood (mean age = 8 years, 6 months) and panic anxiety symptoms in adolescence (mean age = 15 years) increased across independent negative life events. Shared environmental effects on separation anxiety symptoms and nonshared environmental effects on general anxiety symptoms in adolescence were also moderated by negative life events. We interpret these preliminary findings tentatively in the context of gene–environment interaction on anxiety in general, and on early separation and later panic anxiety in particular.
Interactions of child maltreatment and serotonin transporter and monoamine oxidase A polymorphisms: Depressive symptomatology among adolescents from low socioeconomic status backgrounds
Authors: Cicchetti Dante , Rogosch Fred A. , Sturge-Apple Melissa L. 
Published: October 2007

AbstractChild maltreatment and polymorphisms of the serotonin transporter (5-HTT) and monoamine oxidase A (MAOA) genes were examined in relation to depressive symptomatology. Adolescents (Mage = 16.7 years) from low socioeconomic backgrounds with a history of child maltreatment (n= 207) or no such history (n= 132) were interviewed and provided buccal cells for genetic analysis. Gene × environment interactions were observed. Heightened depressive symptoms were found only among extensively maltreated youth with lowMAOAactivity. Among comparably maltreated youth with highMAOAactivity, self-coping strategies related to lower symptoms. Sexual abuse and the5-HTT short/shortgenotype predicted higher depression, anxiety, and somatic symptoms. This Gene × Environment interaction was further moderated byMAOAactivity level. The results highlight the protective functions of genetic polymorphisms and coping strategies in high risk youth and offer direction for understanding resilience and its promotion from a multiple levels of analysis perspective.
Mendelian randomization: A novel test of the gateway hypothesis and models of gene–environment interplay
Authors: Irons Daniel E. , McGue Matt , Iacono William G. , Oetting William S. 
Published: October 2007

AbstractTo determine if drinking behavior in adolescence provides a “gateway” leading to the misuse of other psychoactive substances and antisocial behavior, we genotyped 180 Asian adolescent adoptees to determine if they inherited the deficient from of the aldehyde dehydrogenase 2 (ALDH2) enzyme that is important in the metabolism of alcohol. Based on the gateway model, we hypothesized that those with normal enzyme activity (70% of the sample) who began to misuse alcohol would also misuse other drugs and display antisocial tendencies. Those with the enzyme deficiency (30%), because they experience unpleasant side effects associated with drinking, were expected to show less evidence of alcohol misuse and thus be less likely to progress to the misuse of other substances or engage in antisocial acts. Consistent with previous research, we found that ALDH2 deficiency was significantly associated with lower rates of drinking and getting drunk but not with ever having tried alcohol. Contrary to the gateway model, we found no evidence that ALDH2 deficiency was associated with lower rates of nonalcohol substance use or antisociality. Finally, in an examination of factors that may moderate the impact of the metabolic protection because of ALDH2 deficiency, we identified siblings rather than parents as the major source of familial environmental effect on adolescent drinking.
Working towards ‘An Unforeseen Miracle’ Redux: Latvian Refugees in Vladivostok, 1918–1920, and in Latvia, 1943–1944
Authors: PURS ALDIS 
Published: October 2007

AbstractDuring the First World War the survival of hundreds of thousands of Latvian refugees, dispersed across the Russian Empire, overlapped with issues of identity. Latvians in Siberia and the Far East created a refugee organisation complete with military, diplomatic and cultural programmes for themselves and their homeland. The key players attempted to recreate the same organisational trajectories and outcomes during the Second World War, under very different geopolitical conditions. This article presents new archival research and suggests new interpretations of the dynamic nature of political organisation, refugee experience and identity in Latvia through the first half of the twentieth century.
The Perils of Displacement: The Soviet Evacuee between Refugee and Deportee
Authors: MANLEY REBECCA 
Published: October 2007

AbstractIn the wake of the German invasion of June 1941, sixteen and a half million Soviet citizens were evacuated to the country's interior. Unlike the archetypal European refugee, the Soviet evacuee was displaced but not stateless. This article, based on previously unexamined sources from archives in Russia and Uzbekistan, examines the status of the displaced in a state in which rights were grounded in territory and the lack of fixed residence could have dire implications. More specifically, it focuses on the way in which the evacuee was conceived in relation to the ‘refugee’ and the ‘deportee’.
Displacing and Re-placing Population in the Two World Wars: Armenia and Poland Compared
Authors: GATRELL PETER 
Published: October 2007

AbstractDuring the twentieth century Armenia and Poland alike were sites of widespread population displacement, which brought into sharp focus arguments about national ‘survival’ advanced by patriotic leaders who found in refugees the embodiment of recurrent national suffering. Population displacement also attracted external support from sympathetic foreigners and from the Armenian and Polish diaspora, who regarded it as an affront to civilisation. Among Armenians a groundswell of support for repatriation gathered momentum after both world wars, because Soviet ‘protection’ offered the most realistic chance for national survival. In contrast many Poles opted not to return to Poland after 1945, regarding the communist takeover as a betrayal of Poland's struggle for independence.
Localization of ‘Candidatus Endobugula sertula’ and the bryostatins throughout the life cycle of the bryozoan Bugula neritina
Authors: Sharp Koty H , Davidson Seana K , Haygood Margo G 
Published: October 2007

Abstract
‘Candidatus Endobugula sertula,’ the uncultivated γ-proteobacterial symbiont of the marine bryozoan Bugula neritina, synthesizes bryostatins, complex polyketides that render B. neritina larvae unpalatable to predators. Although the symbiosis is well described, little is known about the locations of ‘E. sertula’ or the bryostatins throughout larval settlement, metamorphosis and early development. In this study, we simultaneously localized ‘E. sertula’ and the bryostatins in multiple stages of the B. neritina life cycle, using a novel bryostatin detection method based on its known ability to bind mammalian protein kinase C. Our results suggest that the bryostatins are deposited onto the exterior of B. neritina larvae during embryonic development, persist on the larval surface throughout metamorphosis and are shed prior to cuticle formation. During metamorphosis, ‘E. sertula’ remains adhered to the larval pallial epithelium and is incorporated into the preancestrula cystid tissue layer, which ultimately develops into a bud and gives rise to the next zooid in the colony. Colocalization of bryostatin signal with aggregates of ‘E. sertula’ in buds of ancestrulae suggested new synthesis of bryostatins in ancestrulae. In adult B. neritina colonies, symbiont microcolonies were observed in the funicular cords of rhizoids, which likely result in asexual transmission of ‘E. sertula’ to regenerated colonies. Furthermore, bryostatin signal was detected on the surface of the rhizoids of adult B. neritina colonies. Through simultaneous localization of the bryostatins and the ‘E. sertula,’ we determined how ‘E. sertula’ is transmitted, and identified shifts in bryostatin localization throughout the life cycle of the host B. neritina.
Soil fungal abundance and diversity: another victim of the invasive plant Centaurea maculosa
Authors: Broz Amanda K , Manter Daniel K , Vivanco Jorge M 
Published: October 2007

Abstract
Interactions between plants and soil microbes are important determinants of both above- and belowground community composition, and ultimately ecosystem function. As exotic plants continue to invade and modify native plant communities, there has been increasing interest in determining the influence of exotic invasives on native soil microbial communities. Here, using highly sensitive molecular techniques, we examine fungal abundance and diversity in the soil surrounding a particularly aggressive invasive plant species in North America, Centaurea maculosa Lam. In mixed stands, we show that this invasive weed can alter the native fungal community composition within its own rhizosphere and that of neighboring native plants. At higher densities, the effect of C. maculosa on native soil fungal communities was even greater. Our results demonstrate that this invasive weed can have significant effects not only on visible aboveground biodiversity but also on the native soil microbial community that extends beyond its rhizosphere.
CellLine, a stochastic cell lineage simulator
Authors: Ribeiro Andre S. , Charlebois Daniel A. , Lloyd-Price Jason 
Published: October 2007

AbstractSummary: We present CellLine, a simulator of the dynamics of gene regulatory networks (GRN) in the cells of a lineage. From user-defined reactions and initial substance quantities, it generates cell lineages, i.e. genealogic pedigrees of cells related through mitotic division. Each cell's dynamics is driven by a delayed stochastic simulation algorithm (delayed SSA), allowing multiple time delayed reactions.The cells of the lineage can be individually subject to ‘perturbations’, such as gene deletion, duplication and mutation. External interventions, such as adding or removing a substance at a given moment, can be specified. Cell differentiation lineages, where differentiation is stochastically driven or externally induced, can be modeled as well. Finally, CellLine can generate and simulate the dynamics of multiple copies of any given cell of the lineage.As examples of CellLine use, we simulate the following systems: cell lineages containing a model of the P53-Mdm2 feedback loop, a differentiation lineage where each cell contains a 4 gene repressilator (a bistable circuit), a model of the differentiation of the cells of the retinal mosaic required for color vision in Drosophila melanogaster, where the differentiation pathway depends on one substance's concentration that is controlled by a stochastic process, and a 9 gene GRN to illustrate the advantage of using CellLine rather than simulating multiple independent cells, in cases where the cells of the lineage are dynamically correlated.Availability: The CellLine program, instructions and examples are available at http://www.cs.tut.fi/~sanchesr/CellLine/CellLine.htmlContact: andre.sanchesribeiro@tut.fi