bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative proteomic study of two closely related ovarian endometrioid adenocarcinoma cell lines using cIEF fractionation and pathway analysis
Authors: Dai Lan , Li Chen , Shedden Kerby A. , Misek David E. , Lubman David M. 
Published: March 2009

AbstractThe proteomic profiles from two distinct ovarian endometrioid tumor‐derived cell lines, (MDAH‐2774 and TOV‐112D) each with different morphological characteristics and genetic mutations, have been studied. Characterization of the differential global protein expression between these two cell lines has important implications for the understanding of the pathogenesis of ovarian endometrioid carcinoma. In this comparative proteomic study, extensive fractionation of peptides generated from whole‐cell trypsin digestion was achieved by coupling cIEF in the first‐dimensional separation with capillary LC (RP‐HPLC) in the second dimensional separation. Online analysis was performed using tandem mass spectra acquired by a linear ion trap mass spectrometer from triplicate runs. A total of 1749 and 1955 proteins with protein probability above 0.95 were identified from MDAH‐2774 and TOV‐112D after filtering through Peptide Prophet/Protein Prophet software. Differentially expressed proteins were further investigated by ingenuity pathway analysis (IPA) to reveal the association with important biological functions. Canonical pathway analysis using IPA demonstrates that important signaling pathways are highly associated with one of these two cell lines versus the other, such as the PI3K/AKT pathway, which is found to be significantly predominant in MDAH‐2774 but not in TOV‐112D. Also, protein network analysis using IPA highlights p53 as a central hub relating to other proteins from the connectivity map. These results illustrate the utility of high throughput proteomics methods using large‐scale proteome profiling combined with bioinformatics tools to identify differential signaling pathways, thus contributing to the understanding of mechanisms of deregulation in neoplastic cells.
A proposed metabolic strategy for monitoring disease progression in Alzheimer's disease
Authors: Greenberg Nicola , Grassano Antonio , Thambisetty Madhav , Lovestone Simon , Legido‐Quigley Cristina 
Published: March 2009

AbstractA specific, sensitive and essentially non‐invasive assay to diagnose and monitor Alzheimer's disease (AD) would be valuable to both clinicians and medical researchers. The aim of this study was to perform a metabonomic statistical analysis on plasma fingerprints. Objectives were to investigate novel biomarkers indicative of AD, to consider the role of bile acids as AD biomarkers and to consider whether mild cognitive impairment (MCI) is a separate disease from AD. Samples were analysed by ultraperformance liquid chromatography–MS and resulting data sets were interpreted using soft‐independent modelling of class analogy statistical analysis methods. PCA models did not show any grouping of subjects by disease state. Partial least‐squares discriminant analysis (PLS‐DS) models yielded class separation for AD. However, as with earlier studies, model validation revealed a predictive power of Q2<0.5 and indicating their unsuitability for predicting disease state. Three bile acids were extracted from the data and quantified, up‐regulation was observed for MCI and AD patients. PLS‐DA did not support MCI being considered as a separate disease from AD with MCI patient metabolic profiles being significantly closer to AD patients than controls. This study suggested that further investigation into the lipid fraction of the metabolome may yield useful biomarkers for AD and metabolomic profiles could be used to predict disease state in a clinical setting.
Microfluidic chips for protein differential expression profiling
Authors: Armenta Jenny M. , Dawoud Abdulilah A. , Lazar Iulia M. 
Published: March 2009

AbstractBiomarker discovery and screening using novel proteomic technologies is an area that is attracting increased attention in the biomedical community. Early detection of abnormal physiological conditions will be highly beneficial for diagnosing various diseases and increasing survivability rates. Clearly, progress in this area will depend on the development of fast, reliable, and highly sensitive and specific sample bioanalysis methods. Microfluidics has emerged as a technology that could become essential in proteomics research as it enables the integration of all sample preparation, separation, and detection steps, with the added benefit of enhanced sample throughput. The combination of these advantages with the sensitivity and capability of MS detection to deliver precise structural information makes microfluidics‐MS a very competitive technology for biomarker discovery. The integration of LC microchip devices with MS detection, and specifically their applicability to biomarker screening applications in MCF‐7 breast cancer cellular extracts is reported in this manuscript. Loading ∼0.1–1 μg of crude protein extract tryptic digest on the chip has typically resulted in the reliable identification of ∼40–100 proteins. The potential of an LC‐ESI‐MS chip for comparative proteomic analysis of isotopically labeled MCF‐7 breast cancer cell extracts is explored for the first time.
Continuous biomass fractionation process for producing ethanol and low‐molecular‐weight lignin
Authors: Kadam Kiran L. , Chin Chim Y. , Brown Lawrence W. 
Published: March 2009

AbstractA continuous biomass fractionation process for producing ethanol and low‐molecular‐weight lignin was developed, and its economic feasibility was evaluated. The two‐stage process fractionates biomass into three streams: a solid cellulose stream and two liquid streams containing mostly hemicellulosic sugars and lignin, respectively. Process optimization was conducted using corn stover as feedstock in a continuous pilot‐scale unit, with the goals of efficient hemicellulose and lignin hydrolysis for the countercurrent first and cocurrent second stage, respectively. Countercurrent prehydrolysis in an extruder is a novel feature of the process. Autohydrolysis at 210°C followed by NaOH (0.06 g/g biomass) catalyzed delignification at 220°C was determined to be optimal. Economic feasibility of the process for ethanol production was compared to that of the National Renewable Energy Laboratory (NREL; Golden, CO) process. Major differences between the two scenarios are that the PureVision process generates lignin as a sellable output but needs to replace the lost fuel value of the lignin with additional stover. The minimum ethanol selling price, calculated following the NREL protocol, is projected to be lower for the PureVision process ($0.94/gal) than that for the NREL process ($1.07/gal)—the differential depending on lignin selling price—indicating a process with commercial potential. © 2009 American Institute of Chemical Engineers Environ Prog, 2009
PBI Composite and Nanocomposite Membranes for PEMFCs: The Role of the Filler
Authors: Quartarone E. , Mustarelli P. , Carollo A. , Grandi S. , Magistris A. , Gerbaldi and C. 
Published: March 2009

AbstractIn spite of the promising properties as polymer electrolytes for fuel cells, H3PO4‐doped PBI systems need to be further optimised for what concerns the oxidative stability, the mechanical performances and the leaching of acid at temperatures lower than 150 °C. To this aim, the preparation of composite and nanocomposite membranes is of interest. Here, we investigate on the actual role of the filler in determining the physical and chemical properties of the membranes. Three types of SiO2 are compared, namely HiSil™ T700, mesoporous SBA‐15 and an imidazole‐functionalised silica, which differ for morphology, microstructure and chemical nature. Particular attention is devoted to the ability of the filler to reduce the drop of conductivity occurring in consequence of the acid leaching. In the as‐doped systems, the proton transport is remarkably improved by the presence of fillers containing active sites for the proton hopping. In the case of washed membranes the acid retention capability and the permanent proton conductivity are increased by fillers with higher surface area or with higher basicity. In the lights of these results, the imidazole‐derivatised silica seems to be an optimal filler for the preparation of PBI membranes for PEMFCs operating at 120 °C.
Bile salt–phospholipid conjugate ursodeoxycholyl lysophosphatidylethanolamide as a hepatoprotective agent #
Authors: Chamulitrat Walee , Burhenne Jürgen , Rehlen Tobias , Pathil Anita , Stremmel Wolfgang 
Published: March 2009

Abstract
A decrease of hepatocellular phosphatidylcholine (PC) is associated with hepatic injury, e.g., in nonalcoholic steatohepatitis (NASH). Therefore, we evaluated the hepatoprotective effect of a PC-precursor lipid specifically targeted to the liver. We synthesized the bile acid-phospholipid conjugate ursodeoxycholyl lysophosphatidylethanolamide (UDCA-LPE), which was designed to target PC to hepatocytes by way of bile-acid transport systems. We synthesized a fluorescently labeled analogue UDCA-6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl PE (UDCA-NBDPE) for uptake and metabolism studies. Unexpectedly, the majority of UDCA-NBDPE was still intact and not hydrolyzed efficiently in HepG2 cells. For targeting in vivo, NBD fluorescence from UDCA-NBDPE-injected mice was recovered in the liver the most, whereas injection of NBDPE alone resulted in an even distribution in liver, kidneys, and intestine. Cytoprotection by UDCA-LPE was tested in starvation and tumor necrosis factor alpha (TNF-α) apoptosis models using HepG2 cells. Only the intact UDCA-LPE was able to persistently stimulate growth after 36 to 120-hour starvation, and significantly inhibited TNF-α-induced apoptosis. In both models, LPC, LPE, UDCA, or UDCA added with LPE exhibited weak to no cytoprotection. UDCA-LPE stabilized mitochondrial membranes by lowering mitochondrial membrane potential. Western blot analyses of phosphorylated Akt and glycogen synthase kinase-3 (GSK-3)α/β revealed that UDCA-LPE activated phosphatidyl inositol 3-kinase (PI3K)/Akt signaling pathways. The PI3K inhibitor LY294002 or Akt small interfering (si)RNA consistently inhibited the proproliferative effects of UDCA-LPE during starvation. The TNF-α death-receptor extrinsic pathway involves caspase 8 activation, which is inhibited by cellular FLICE-inhibitory protein (cFLIP); thus, cFLIP siRNA was employed in our studies. cFLIP siRNA was able to reverse the cytoprotective effects of UDCA-LPE during TNF-α-induced apoptosis, and UDCA-LPE concomitantly upregulated protein expression of cFLIPL. Conclusion: UDCA-LPE, which targeted the liver in vivo, elicited potent biological activities in vitro by stimulating hepatocyte growth and by inhibiting TNF-α-induced apoptosis. Thus, UDCA-LPE may be suitable for evaluation of treatment efficacy in NASH. (Hepatology 2009.)
Risk of catastrophic terrorism: an extreme value approach
Authors: Mohtadi Hamid , Murshid Antu Panini 
Published: March 2009

AbstractThis paper models the stochastic behavior of large‐scale terrorism using extreme value methods. We utilize a unique dataset composed of roughly 26,000 observations. These data provide a rich description of domestic and international terrorism between 1968 and 2006. Currently, a credible worst‐case scenario would involve losses of about 5000 to 10,000 lives. Also, the return time for events of such magnitude is shortening every year. Today, the primary threat is from conventional weapons, rather than from chemical, biological and/or radionuclear weapons. However, pronounced tails in the distribution of these incidents suggest that this threat cannot be dismissed. Copyright © 2009 John Wiley & Sons, Ltd.
The effect on bone growth enhancement of implant coatings with hydroxyapatite and collagen deposited electrochemically and by plasma spray
Authors: Daugaard Henrik , Elmengaard Brian , Bechtold Joan E. , Jensen Thomas , Soballe Kjeld 
Published: March 2009

AbstractSkeletal bone consists of hydroxyapatite (HA) [Ca10(PO4)6(OH)2] and collagen type I, both of which are osseoconductive. The goal of osseointegration of orthopedic and dental implants is the rapid achievement of a mechanically stable long‐lasting fixation between bone and an implant surface. In this study, we evaluated the mechanical fixation and tissue distribution surrounding implants coated with three surfaces: plasma‐sprayed HA coating, thinner coating of electrochemical‐assisted deposition of HA, and an identical thin coating with a top layer of mineralized collagen. Uncoated plasma‐sprayed titanium (Ti‐6Al‐4V) served as negative control. The electrochemical‐assisted deposition was performed near physiological conditions. We used a canine experimental joint replacement model with four cylindrical implants (one of each treatment group) inserted in the humeri cancellous metaphyseal bone in a 1 mm gap. Observation time was 4 weeks. The mechanical fixation was quantified by push‐out test to failure, and the peri‐implant tissue formation by histomorphometric evaluation. HA coatings deposited by plasma spray technique or electrochemically, increased the mechanical fixation and bone ongrowth, but there was no statistical difference between the individual HA applications. Addition of collagen to the mineralized phase of the coating to create a more bone natural surface did not improve the osseoconductive effect of HA. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Evaluating antimicrobials and implant materials for infection prevention around transcutaneous osseointegrated implants in a rabbit model
Authors: Chou Teri G. Rosenbaum , Petti Cathy A. , Szakacs Juliana , Bloebaum Roy D. 
Published: March 2009

AbstractTranscutaneous osseointegrated implants can improve function for select amputee patients, but infection serves as a significant limitation of implantable transcutaneous devices. This study examined the efficacy of an antimicrobial, pexiganan acetate (SUPONEX™), and a porous tantalum implant material (Trabecular Metal™) in preventing pin tract infection of osseointegrated implants in a rabbit model. Thirty‐seven rabbits were randomized to three groups: Ti‐control group (n = 11) with titanium alloy implant and no antimicrobial, Ti‐Pexiganan group (n = 8) with titanium alloy implant and topical pexiganan acetate 1% applied daily at the skin/implant interface, and Ta‐control group (n = 18) with porous tantalum implant and no antimicrobial. All implants were placed transcutaneously through skin, muscle, and bone. Rabbits were monitored for infection for 24 weeks. We observed a 75% reduction in rates of pin tract infection in the Ti‐Pexiganan group compared to that observed in the Ti‐control group (p = 0.019). No difference in rates of infection was observed between the Ta‐control group and the Ti‐control group (p = 0.230). In conclusion, pexiganan acetate may be an important antimicrobial for transcutaneous osseointegrated implants. Porous tantalum will not likely prevent pin tract infection without additional methods of soft tissue immobilization around the implant site. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Novel multiarm PEG‐based hydrogels for tissue engineering
Authors: Tan Huaping , DeFail Alicia J. , Rubin J. Peter , Chu Constance R. , Marra Kacey G. 
Published: March 2009

AbstractInjectable scaffolds are promising substrates for regenerative medicine applications. In this study, multiarm amino‐terminated poly(ethylene glycol) (PEG) hydrogels were crosslinked with genipin, a compound naturally derived from the gardenia fruit. Four‐ and eight‐arm amino‐terminated PEG hydrogels crosslinked with varying concentrations of genipin were characterized. Both surface and cross‐sectional structures of PEG‐based hydrogels were observed by scanning electron microscopy. In vitro gelation time, water uptake, swelling, and weight loss of PEG hydrogels in phosphate buffered saline at 37°C were studied. The results showed that the eight‐arm PEG demonstrated a much slower gelation time compared with the four‐arm PEG, which may be due to the differing structures of the multiarm PEG hydrogels, which in turn affects the ability of genipin to react with the amine groups. Human adipose‐derived stem cells were seeded onto the four‐ and eight‐arm PEG hydrogels in vitro to assess the biological performance and applicability of the gels as cell carriers. The four‐arm PEG hydrogel resulted in enhanced cell adhesion when compared with the eight‐arm PEG hydrogel. Overall, these characteristics provide a potential opportunity for multiarm PEG hydrogels as injectable scaffolds in a variety of tissue engineering applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Performance of plane‐wave‐based LDA+U and GGA+U approaches to describe magnetic coupling in molecular systems
Authors: Rivero Pablo , Loschen Christoph , Moreira Ibério De P. R. , Illas Francesc 
Published: March 2009

AbstractThis work explores the performance of periodic plane wave density functional theory calculations with an on‐site Coulomb correction to the standard LDA and GGA exchange‐correlation potential—commonly used to describe strongly correlated solids—in describing the magnetic coupling constant of a series of molecular compounds representative of dinuclear Cu complexes and of organic diradicals. The resulting LDA+U or GGA+U formalisms, lead to results comparable to experiment and to those obtained by means of standard hybrid functionals provided that the value of the U parameter is adequately chosen. Hence, these methods offer an alternative efficient computational scheme to correct LDA and GGA approaches to adequately describe the electronic structure and magnetic coupling in large molecular magnetic systems, although at the expenses of introducing an empirical (U) parameter. For all investigated copper dinuclear systems, the LDA+U and GGA+U approaches lead to an improvement in the description of magnetic properties over the original LDA and GGA schemes with an accuracy similar to that arising from the hybrid B3LYP functional, by increasing the on‐site Coulomb repulsion with a moderate U value. Nevertheless, the introduction of an arbitrary U value in the 0–10 eV range most often provides the correct ground‐state spin distribution and the correct sign of the magnetic coupling constant. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2009
Synthesis and laser activity of halo‐acridinedione derivatives
Authors: Kaya Muharrem , Yıldırır Yılmaz , Türker Lemi 
Published: March 2009

Abstract
magnified imageSynthesis of 10‐(halophenyl)‐9‐(4‐methoxyphenyl)‐3,4,6,7,9,10‐hexahydroacridine‐1,8‐(2H,5H)‐dione derivates have been prepared and their absorption, emission, and laser properties have been evaluated. The structures of all the synthesized compounds were characterized by spectroscopic methods IR, 1H NMR, 13C‐APT, MS, and elemental analysis. J. Heterocyclic Chem., (2009).
Retro‐ene reactions in heterocyclic synthesis. II. A facile synthetic method for 4‐hydroxy‐2‐pyridones
Authors: Ito Kunio , Miyajima Shingo 
Published: March 2009

AbstractSchiff's bases 1 derived from ketones and t‐butylamine reacted with diphenyl malonate, diphenyl methyl‐malonate and diphenyl phenylmalonate to give 4‐hydroxy‐2‐pyridones 4–6. Schiff's bases b on reaction with trimethyl methanetricarboxylate afforded 4‐hydroxy‐3‐methoxycarbonyl‐2‐pyridones 12.
The first synthesis of pyrido[2,1‐a]phthalazinium system and its three thieno‐fused analogues
Authors: Gronowitz Salo , Messmer András , Timári Géza 
Published: March 2009

AbstractSubstituted biaryl compounds 9, 14–16 were synthesized through Pd(0)‐catalyzed cross coupling reactions between boronic acids or tin derivatives and aryl halides. N‐Amination, and subsequent ring closure resulted in the new angularly‐fused pyrido[1,2‐b]pyridazinium systems 1–4. The use of silver oxide as a cocatalyst in the couplings of tin derivatives was essential in order to obtain rapid reaction and better yields. Structures were determined by 1H nmr and 13C nmr spectra.
Cyclization of N‐cyanovinyl‐lactam imines to condensed 4‐aminopyridines or substituted aminopyrazole
Authors: Pätzel Michael , Ushmajev Alexej , Liebscher Jürgen , Granik Vladimir , Grisik Sofia , Polievktov Mikhail 
Published: March 2009

AbstractN‐Cyanovinyl‐lactam imines 2 react with hydrazine giving aminopyrazole 4a while lactam imine 3 is split off. Treatment of 2 with strong bases gives intramolecular cyclization to condensed 4‐aminopyridines 5. The progress of reaction was observed by polarographic method.
Palladium‐ or ruthenium‐catalyzed synthesis of 2‐phenylindoles
Authors: Izumi Taeko , Yokota Toshiko 
Published: March 2009

AbstractAllyl α‐phenyl‐2‐aminophenethyl carbonates undergo a smooth decarboxylation‐dehydrogenation reaction to afford 2‐phenylindole derivatives in acetonitrile at 80° in the presence of palladium complex as catalyst. In the reaction, the ruthenium hydride complex showed more effective catalytic activities. 2‐Phenylindoles were also prepared from the corresponding α‐phenyl‐2‐aminophenethylalcohols and allyl methyl carbonate by ruthenium‐catalyzed cyclization.
Synthesis of 2,10‐diphenyl‐2H‐pyridazino[4,5‐b]quinolin‐1‐one and 2,3‐dihydro‐9‐phenyl‐2‐phenylamino‐1H‐pyrrolo[3,4‐b]quinolin‐1‐one derivatives as peripheral‐type benzodiazepine receptor ligands
Authors: Anzini M. , Cappelli A. , Vomero S. , Cagnotto A. , Skorupska M. 
Published: March 2009

AbstractThe reaction of 2‐chloromethyl‐3‐ethoxycarbonyl‐4‐phenylquinoline with phenylhydrazine, afforded 2,3‐dihydro‐9‐phenyl‐2‐phenylamino‐1H‐pyrrolo[3,4‐b]quinolin‐1‐one derivatives2along with a major amount of 3‐ethoxycarbonyl‐4‐phenylquinoline‐2‐carboxaldehyde phenylhydrazone4a. The geometric isomers of phenylhydrazone4a, displaying solvent‐dependentE‐Zisomerism, were isolated, characterized by1H‐nmr and mass spectra, and theZ‐form easily cyclized to pyridazino[4,5‐b]quinoline derivative5a. Analogously, compounds2b, 2c, 4b,4c, 5band5cwere obtained. The title compounds were tested as potential ligands for central and peripheral‐type of benzodiazepine receptors, and the results are reported.
Reaction of N,N'‐dialkyldithiodianilines with β‐ketoesters. A new synthesis of N‐alkyl‐2‐acyl‐3‐oxo‐3,4‐dihydro‐1,4‐benzothiazines
Authors: Trapani Giuseppe , Latrofa Andrea , Reho Antonia , Franco Massimo , Liso Gaetano 
Published: March 2009

AbstractThe title compounds 3a‐j together with the N‐alkylacylketene S,N‐acetals 12a‐j were obtained by reaction of N,N'‐dialkyldithiodianilines with β‐ketoesters compounds. A possible reaction pathway is suggested.
Tricyclic heteroaromatic systems. 1,2,4‐triazolo[1,5‐a]quinoxaline
Authors: Catarzi Daniela , Cecchi Lucia , Colotta Vittoria , Filacchioni Guido , Melani Fabrizio 
Published: March 2009

AbstractThe parent 1,2,4‐triazolo[1,5‐a]quinoxaline was prepared by a few‐step reaction from ethyl N1‐(2‐nitrophenyl)‐N3‐ethoxalyloxamidrazonate, which afforded the key intermediate diethyl 1‐(2‐nitrophenyl)‐1,2,4‐triazole‐3,5‐dicarboxylate in satisfactory yield. The latter was cyclized, hydrolyzed, decarboxylated, reduced and dehydrogenated to yield the title compound.
Formation of heterocyclic products in michael reactions of ascorbic acid with α,β‐unsaturated compounds. 3rd communication . Structural analysis of michael adducts of ascorbic acid by 13C‐nmr spectroscopy in the solid state
Authors: Eger Kurt , Schmidt Mathias , Albert Klaus , Schmid Jutta 
Published: March 2009

AbstractThe structure of the Michael adducts of ascorbic acid (AA) and AA‐6‐palmitate with acrolein can be unambiguously determined by using 13C‐nmr solid state spectroscopy.