bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel [Ag(NH3)2]+ probe for chemiluminescent imaging detection of proteins after polyacrylamide gel electrophoresis
Authors: Xiong Xin , Wang Zhenzhen , Baeyens Willy R. G. , Delanghe Joris R. , Huang Zhi , Huang Guangming , Ouyang Jin 
Published: July 2007

AbstractThe development of a novel [Ag(NH3)2]+ probe chemiluminescence (CL)‐based imaging method for the detection of various proteins after PAGE is described. The detection is based upon the probe [Ag(NH3)2]+ catalyzing the CL reaction of the luminol–potassium persulfate system. The proposed method detects various proteins labeled by [Ag(NH3)2]+ and expands the application scope to SDS gels. It also detects proteins directly in polyacrylamide gels, without tedious transferring procedures. Furthermore, successful identification of proteins by peptide mass profiling using ionization MS was easily performed, and no pretreatments of gel prior to digestion are needed. Detection limits for standard marker proteins match CBB‐R250 staining and the linear dynamic range is superior to CBB‐R250 staining and silver staining. The CL imaging conditions, including luminescent reagents, silver ion concentration, the ammonia‐controlled system and the washing reagents parameters have also been optimized.
Adduction of solvent molecules by ions isolated within an ion trap mass spectrometer under atmospheric pressure ionisation conditions
Authors: Wickens James R. , Sleeman Richard , Keely Brendan J. 
Published: July 2007

AbstractBenzylpyridine and papaverine, an alkyl quinoline, both produce product ions containing an azepinium ring during atmospheric pressure chemical ionisation or electrospray multistage mass spectrometry. By controlling the trapping conditions, an isolated azepinium ion was held within the trap for an extended period of time without excitation. A subsequent analytical scan revealed a mass spectrum containing ions at two mass‐to‐charge (m/z) ratios, the first at the m/z of the isolated product ion and the second at an m/z ratio corresponding to the adduction of a molecule of solvent. Isolation and resonance excitation of the adduct ion remove the solvent molecule, resulting in recovery of the azepinium ion at the same signal intensity as the adduct ion. Isolating and trapping the ion for a further period allowed the solvent adduct ion to be re‐formed. Modulation of the solvent flowing into the source while the ion was trapped allowed variation in the solvent molecule adducted to the trapped ion. The proportion of the ion current due to the adduct ion depends on the nature of the isolated ion, the proton affinity of the solvent and the length of time for which the ion was trapped. Adduct ion formation, deliberately maximised in this study, can occur to a significant extent under standard ion trap operating conditions, reducing the ion current of product ions of interest and, ultimately, the response in tandem mass spectrometric assays. Copyright © 2007 John Wiley & Sons, Ltd.
Tracking human travel using stable oxygen and hydrogen isotope analyses of hair and urine
Authors: O'Brien Diane M. , Wooller Matthew J. 
Published: July 2007

AbstractThe stable oxygen and hydrogen isotope compositions of organic samples are increasingly being used to investigate patterns of animal migration. Relatively few studies have applied these techniques to modern humans, despite a variety of potential forensic applications. We analyzed drinking water and food at two geographic locations, East Greenbush, New York (USA) and Fairbanks, Alaska (USA), with different δ18O and δD values for precipitation and tap water. Foods varied widely in measured δ18O and δD values, but not systematically by purchase location. We measured δ18O and δD values of scalp hair from five residents at each location. We used a mixing model to establish the proportion of oxygen and hydrogen in head hair derived from drinking water (∼27% and ∼36%, respectively). Finally, we analyzed the δ18O and δD values of facial hair and urine from a subject who traveled from Fairbanks to East Greenbush, on to the UK and back to Fairbanks. Urine δ18O and δD values responded immediately and strongly to travel‐related change in drinking water, and were well described by a single‐pool turnover model. Beard hair δ18O values tracked changes in urine δ18O closely, and oscillated between the values for the resident populations in both locations. In contrast, beard hair δD values did not track changes in urine δD as well, and retained a signature of the traveler's permanent residence. Our findings show that the δ18O and δD values of urine and facial hair (specifically δ18O) can provide a record of the geographical movements of humans. Copyright © 2007 John Wiley & Sons, Ltd.
Open tube combustion method of organic samples for stable carbon isotope analysis
Authors: Velivetskaya Tatiana A. , Ignatyev Alexander V. , Reize Marina V. , Kiyashko Serguei I. 
Published: July 2007

AbstractA simple and effective method for the conversion of organic carbon into carbon dioxide for analysis of stable carbon isotopes (δ13C) in samples of various organic substances, soils, sedimentary rocks, oils and volatile organic liquids is presented. The conversion of organic carbon of the samples is carried out in a quartz reactor connected to a vacuum line for CO2 freezing and purification. A solid organic sample mixed with CuO is placed at the reactor bottom and the reactor is subsequently filled with granular CuO. One end of the CuO column is preheated to 850°C while the other end of the column in contact with the sample is kept at ambient temperature. Heating of the sample (850°C) and the remainder of the column is then performed. The preheated part of the column provides efficient conversion of carbon into CO2. The reactor for the conversion of volatile liquid organic compounds is filled with granular CuO. The column of CuO is heated to 850°C. Samples of volatile liquids are introduced into the reactor through a septum using a microsyringe. Complete conversion takes 10 min for solid samples and 3 min for volatile liquids. The precision of the δ13C analysis for solid and volatile liquid organic substances is ±0.1‰ and ±0.04‰, respectively. Copyright © 2007 John Wiley & Sons, Ltd.
Outcomes and prognostic factors of cirrhotic patients with hepatocellular carcinoma after radical major hepatectomy
Authors: Zhou Li , Rui Jing‐An , Wang Shao‐Bin , Chen Shu‐Guang , Qu Qiang , Chi Tian‐Yi , Wei Xue , Han Kai , Zhang Ning , Zhao Hai‐Tao 
Published: July 2007

AbstractBackgroundRadical major hepatectomy (RMH) has been suggested as one of main options for cure of large/advanced hepatocellular carcinoma (HCC). However, its operative risk remains high and its effectiveness is still controversial, especially for patients with liver cirrhosis. The present study aims to investigate short‐ and long‐term outcomes and to identify prognostic factors for cirrhotic patients with HCC after RMH.Materials and MethodsProspectively collected clinicopathological data of 81consecutive cirrhotic HCC patients who underwent RMH were reviewed retrospectively. The Kaplan‐Meier method was adopted for evaluating long‐term survival. Prognostic factors were identified by univariate and multivariate analyses.ResultsAfter RMH, perioperative mortality, overall morbidity, and life‐threatening morbidity were 1.2%, 24.7%, and 12.3%, respectively. Overall and disease‐free 5‐year survival rates were 39.4% and 28.1%, respectively. Univariate analysis showed that presence of portal vein tumor thrombosis (PVTT) and satellite nodules, late TNM staging, high Edmondson‐Steiner grading, and blood transfusion was associated with worsened prognosis. Of them, Edmondson‐Steiner grading was identified as the sole independent prognostic factor for both overall and disease‐free survival by multivariate analysis, whereas blood transfusion and the presence of PVTT independently predicted unfavorable overall or disease‐free survival, respectively.ConclusionsThese data indicated that RMH was safe and appeared to be effective in treating cirrhotic patients with HCC. Some tumor‐related and clinical variables influenced long‐term outcome of these patients after RMH.
Liver Resection for Hepatocellular Carcinoma in a Hepatitis B Endemic Area
Authors: Bartlett Adam St. J. R. , McCall John L. , Koea Jonathan B. , Holden Andrew , Yeong Mee‐Ling , Gurusinghe Nishanthi , Gane Ed 
Published: July 2007

AbstractBackgroundHepatocellular carcinoma (HCC) is the most common primary malignancy of the liver. Treatment options include liver resection, tumor ablation, and liver transplantation.MethodsWe report the results of all patients undergoing partial hepatectomy for HCC with curative intent from a center where all major treatment modalities were available.ResultsA series of 53 patients were identified, of whom 72% had underlying liver disease, mostly chronic hepatitis B infection. Altogether, 57% of patients underwent major resections, of whom 43% had histologically proven cirrhosis. Postoperative morbidity and mortality occurred in 41.5% and 7.5%, respectively. After a median follow‐up of 34 months, the survival probabilities at 1, 3, and 5 years were 74.1%, 54.1%, and 42.6%, respectively. A total of 47% developed recurrent disease over the study period with a median disease‐free survival of 13.8 months. The probabilities of recurrence at 1, 3, and 5 years were 35.2%, 49.4%, and 55.9%, respectively. Among those who developed recurrence, 76% died, with a median time to death from the time the recurrence was diagnosed of 7.8 months. There was a good association between the CLIP score and survival following liver resection. Multivariate analysis showed that only tumor recurrence and the presence of cirrhosis was a significant determinant of the risk of tumor‐related death.ConclusionThese findings confirm that with careful patient selection liver resection for HCC can achieve good long‐term patient survival and acceptable risks.
Open Mesh Repair of Incisional Hernia Using a Sublay Technique: Long‐Term Follow‐up
Authors: Kurzer Martin , Kark Allan , Selouk Simon , Belsham Philip 
Published: July 2007

AbstractBackgroundReported results of incisional hernia repair are poor with high recurrence rates unless prosthetic mesh is used. Mesh gives improved results, but certain techniques are associated with a high incidence of infections, fistulas, and seromas. This study reports the results of a consecutive series of incisional hernias repaired using an open sublay technique with retromuscular mesh placement. The primary endpoint was hernia recurrence. Secondary endpoints were complications and long‐term discomfort.MethodsA total of 125 patients were operated on between 1991 and 2001. In 2002 they were sent a questionnaire and asked to return for examination if they thought their hernia had recurred or if they had pain. A second questionnaire was sent in 2005, and all patients were asked to return for examination.ResultsThere were no postoperative deaths and no major systemic complications. There were no early (within 30 days) wound infections; the mesh subsequently became infected in two patients and had to be removed. Seromas developed in 12 patients. In 2002, a total of 106 questionnaires were returned; 3 patients had died of unrelated causes, and 16 were untraceable despite repeated attempts. There were five (4%) recurrences. Altogether, 6 patients had abdominal wall discomfort, and 49 patients spontaneously wrote that they were pleased or very pleased with the long‐term result. At a second follow‐up a mean of 8 years after operation (95 months; range 46–168 months) patients were assessed by an independent observer, and there were no further recurrences.ConclusionsOpen repair of incisional hernias with mesh in the subfascial plane is highly effective with acceptable complication rates. Surgeon experience and a team approach are important factors in obtaining good results. Trials comparing open with laparoscopic repair are needed.
In Situ Intestinal Ischemia‐Reperfusion Injury in the Pig: A Model Using the First Jejunal Artery for Flushing
Authors: Yandza Thierry , Mekaouche Mourad , Bréaud Jean , Oroboscianu Ioana , Saint‐Paul Marie‐Christine , Ramella‐Virieux Silvina , Benchimol Daniel , Gugenheim Jean 
Published: July 2007

AbstractBackgroundWe describe a new surgical technique of in situ intestinal ischemia–reperfusion injury in the pig, which includes transection of the small bowel, extrinsic autonomic denervation, lymphatic disruption, and finally in‐situ cold ischemia of the graft by flushing through the first jejunal artery.Material and methodsTen female pigs were used for the study. All neural and lymphatic connections to the jejunoileum were transected. The stripped superior mesenteric vessels remained as the only connections. The skeletonized mesenteric vessels were clamped and the superior mesenteric artery was cannulated through the first jejunal artery. The isolated jejunoileum was flushed with cold IGL‐1 solution. A small incision on the superior mesenteric vein was made to allow outflow of the effluent. After the flushing process was complete, the small incision in the superior mesenteric vein was closed and the vascular clamps were removed. The proximal 70% of the graft was resected.ResultsThe mean preoperative weight of the animals was 25.8 ±7.6 kg. The mean duration of the operation was 242.0 ± 28.6 min. The mean cold ischemia time was 47.6 ± 3.9 min. All animals survived the procedure and were sacrificed at day 8. At sacrifice, there were no adhesions. The small bowel appeared normal. On intestinal histology, there were no significant changes between specimens obtained from the animal immediately at the end of cold flushing (T0), 2 h after reperfusion (T1), and at sacrifice (T2).ConclusionsThis novel technique for intestinal ischemia–reperfusion injury in the pig provides an extremely useful model for experimental studies of immunological and cold ischemia–reperfusion injury of transplanted small bowels.
Decreased Fibroblast and Increased Osteoblast Functions on Ionic Plasma Deposited Nanostructured Ti Coatings
Authors: Cohen Ariel , Liu-Synder Peishan , Storey Dan , Webster Thomas J 
Published: July 2007

Abstract
Bioactive coatings are in high demand to control cellular functions for numerous medical devices. The objective of this in vitro study was to characterize for the first time fibroblast (fibrous scar tissue forming cells) adhesion and proliferation on an important polymeric biomaterial (silicone) coated with titanium using a novel ionic plasma deposition (IPD) process. Fibroblasts are one of the first anchorage-dependent cells to arrive at an implant surface during the wound healing process. Persistent excessive functions of fibroblasts have been linked to detrimental fibrous tissue formation which may cause implant failure. The IPD process creates a surface-engineered nanostructure (with features usually below 100 nm) by first using a vacuum to remove all contaminants, then guiding charged metallic ions or plasma to the surface of a medical device at ambient temperature. Results demonstrated that compared to currently used titanium and uncoated silicone, silicone coated with titanium using IPD significantly decreased fibroblast adhesion and proliferation. Results also showed competitively increased osteoblast (bone-forming cells) over fibroblast adhesion on silicone coated with titanium; in contrast, osteoblast adhesion was not competitively increased over fibroblast adhesion on uncoated silicone or titanium controls. In this manner, this study strongly suggests that IPD should be further studied for biomaterial applications in which fibrous tissue encapsulation is undesirable (such as for orthopedic implants, cardiovascular components, etc.).
Care related pain in hospitalized patients: A cross‐sectional study
Authors: Coutaux Anne , Salomon Laurence , Rosenheim Michel , Baccard Anne‐Sophie , Quiertant Catherine , Papy Emmanuelle , Blanchon Thierry , Collin Elisabeth , Cesselin François , Binhas Michèle , Bourgeois Pierre ,   The Pain Committee, Hôpital de la Pitié‐Salpêtrière, AP‐HP, Paris, France
Published: July 2007

AbstractContext: Care‐related pain includes pain occurring during transportation, movement, diagnostic imaging, physical examination, or treatment. Its prevalence has never been assessed in a large adult inpatient population.Objective: To identify the procedures likely to induce or increase pain in hospital patients, attempting to separate the most painful from those reported as most frequently inducing pain.Design: A single‐day cross‐sectional survey conducted in two large French teaching hospitals, including all hospitalized patients, free of communication problems. One third was randomly selected and interviewed about the painful episodes that had occurred or were associated with the procedures performed during the previous two weeks. Patients were interviewed using a structured questionnaire.Results: Six‐hundred‐eighty‐four patients were randomly selected. Six‐hundred‐seventy‐one painful events were reported in 55% of the patients, with an average of 1.8events/patient. Fifty‐two percent of the painful events were associated with procedures performed by non‐medical staff; 38% of the painful episodes occurred during procedures involving vascular puncture and 24% during patients’ mobilization. In 57% of painful procedures, pain was rated as severe or extremely severe. The most painful procedures were invasive procedures, other than vascular and non vascular punctures (74% of severe and extremely severe painful episodes). Maximum pain intensity was rated higher for procedures that were repeated than for those experienced only once (62% versus 53%, p=0.02).Conclusion: This survey gives new insight into our daily practice. Proper management of care‐related pain should be a major concern of all hospital staff to improve the quality of our health care.
Influence of scapular position on the pressure pain threshold of the upper trapezius muscle region
Authors: Azevedo Daniel Camara , de Pires Tatiana Lima , de Andrade Fernanda Souza , McDonnell Mary Kate 
Published: July 2007

AbstractA position of scapula depression will maintain the upper trapezius muscle region in a lengthened position, causing excessive strain. This strain could lead to peripheral nociceptive nerves sensitization in the affected area, changing the pressure pain threshold (PPT). Thus, people with a faulty alignment of scapular depression may have lower PPT levels in the upper trapezius region when compared to subjects with normal vertical scapular position. The purpose of this double‐blind study was to assess the influence of scapular position on the PPT of the upper trapezius region in a young healthy population. Fifty two physical therapy students of the Catholic University of Minas Gerais – PUC‐Minas, Brazil, with normal shoulder (NS group,n=26, 6 men and 20 women) or depressed shoulder (DS group,n=26, 6 men and 20 women) volunteered to participate in this study. An electronic pressure algometer was used to measure the PPT on the upper trapezius muscle region. The results showed a significant difference between groups, with the DS group (19.0±9.0N/cm2) demonstrated lower mean PPT values when compared to NS group (26.1±9.6N/cm2) (p<0.01). Our results showed that healthy young subjects with depressed scapula position had significant lower upper trapezius PPT values when compared to subjects with normal scapula position.
Paraspinal abscess complicated by endocarditis following a facet joint injection⋆
Authors: Hoelzer Bryan C. , Weingarten Toby N. , Hooten W. Michael , Wright R. Scott , Wilson Walter R. , Wilson Peter R. 
Published: July 2007

AbstractInfectious complications secondary to lumbar facet injections are exceedingly rare, follow an indolent course, and local sequelae include abscess spread or infections of the central nervous system. We present the case of the development of a facet abscess and infective endocarditis, which developed shortly after a lumbar facet injection. With the increase in interventional pain procedures, physicians must be aware of potential infectious complications.
Long‐term pain after inguinal hernia repair in a population‐based cohort; risk factors and interference with daily activities
Authors: Kalliomäki Maija‐L. , Meyerson Josefine , Gunnarsson Ulf , Gordh Torsten , Sandblom Gabriel 
Published: July 2007

AbstractIn the Swedish Hernia Register 2834 inguinal hernia repairs in 2583 patients were registered in the county of Uppsala 1998–2004. In May 2005 the 2421 patients still alive were requested by mail to fill in a validated questionnaire concerning postherniorrhaphy pain. The final response rate became 72%. Altogether 519 patients (29%) stated that they had pain in the operated groin to some extent during past week. In 98 patients (6%) the pain interfered with daily activities. Factors associated with an increased risk of residual pain in a multivariate logistic regression analysis were age below median, operation for recurrence, open repair technique, history of preoperative pain, and less than three years from surgery. Factors not associated with occurrence of residual pain were gender, method of anaesthesia during surgery, hernia sac diameter, postoperative complications, hernia type, need for emergency operation, reducibility of the hernia sac and complete dissection of the hernia sac. Factors found to be associated with impairment of function due to pain in a multivariate logistic regression analysis were: age below median, female gender, medial hernia, open repair technique, postoperative complications, need for operation for recurrence, presence of preoperative pain and less than three years from surgery. The possibility of long‐term pain as an outcome after hernia operations should be taken into consideration in the decision making prior to operation.
Late Quaternary environmental and human Events at En Gedi, reflected by the geology and archaeology of the Moringa Cave (Dead Sea area, Israel)
Authors: Lisker Sorin , Porat Roi , Davidovich Uri , Eshel Hanan , Lauritzen Stein-Erik , Frumkin Amos 
Published: July 2007

AbstractThe Moringa Cave within Pleistocene sediments in the En Gedi area of the Dead Sea Fault Escarpment contains a sequence of various Pleistocene lacustrine deposits associated with higher-than-today lake levels at the Dead Sea basin. In addition it contains Chalcolithic remains and 5th century BC burials attributed to the Persian period, cemented and covered by Late Holocene travertine flowstone. These deposits represent a chain of Late Pleistocene and Holocene interconnected environmental and human events, echoing broader scale regional and global climate events. A major shift between depositional environments is associated with the rapid fall of Lake Lisan level during the latest Pleistocene. This exposed the sediments, providing for cave formation processes sometime between the latest Pleistocene (ca. 15 ka) and the Middle Holocene (ca. 4500 BC), eventually leading to human use of the cave. The Chalcolithic use of the cave can be related to a relatively moist desert environment, probably related to a shift in the location of the northern boundary of the Saharo-Arabian desert belt. The travertine layer was U–Th dated 2.46"0.10 to 2.10"0.04 ka, in agreement with the archaeological finds from the Persian period. Together with the inner consistency of the dating results, this strongly supports the reliability of the radiometric ages. The 2.46–2.10 ka travertine deposition within the presently dry cave suggests a higher recharge of the Judean Desert aquifer, correlative to a rising Dead Sea towards the end of the 1st millennium BC. This suggests a relatively moist local and regional climate facilitating human habitation of the desert.