bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inverse design of periodic microstructures with targeted nonlinear mechanical behaviour
Authors: Thillaithevan Dilaksan , Murphy Ryan , Hewson Robert , Santer Matthew 
Published: March 2024

AbstractThis paper introduces an inverse design framework for the precise tailoring of desired nonlinear mechanical responses in periodic microstructures, with particular focus on prescribed nonlinear stress–strain relationships. The topology optimization hinges on minimizing the error between the target and realized properties of the microstructures. A deformation-driven homogenization framework is setup. The periodic constraints needed for the microscale equilibrium equation are imposed through strongly enforced periodic boundary conditions and the removal of the translational nullspace, avoiding the need for Lagrange multipliers, greatly simplifying the implementation. Automatic differentiation is leveraged to efficiently calculate the necessary sensitivities for the gradient-based optimization. To further aid the design of discrete designs a intermediate density penalty constraint is proposed. Numerical examples underscore the efficacy of our methodology, showcasing microstructures that demonstrate targeted softening and stiffening as well as distinctive directional behaviour.
Foreign direct investment and inclusive finance: do financial markets and quality of institutions matter?
Authors: Abor Joshua Yindenaba , Dwumfour Richard Adjei , Agbloyor Elikplimi Komla , Pan Lei 
Published: March 2024

AbstractWe examine the impact of foreign direct investment (FDI) on financial inclusion. To identify the causal effect of FDI on financial inclusion, we use plausibly exogenous source of variations in bilateral investment treaties (BITs) as a novel instrumental variable (IV) for net FDI inflows. Using annual data for a broad panel of 90 countries over the period 2004 to 2017, our results show that FDI improves financial inclusion for both “access to finance" and “use of financial services". This impact is more pronounced for relatively poor countries and developing countries compared to rich and developed countries. We also find that higher financial market development and quality institutions improve financial inclusion directly. Moreover, financial market development and institutional quality can serve as potential channels and moderating variables through which FDI affects financial inclusion. Our results are robust to various estimations and sample splitting and have important implications for policy on financial inclusion.
Mosapride stimulates human 5-HT4-serotonin receptors in the heart
Authors: Neumann Joachim , Hesse Christin , Hofmann Britt , Gergs Ulrich 
Published: March 2024

AbstractMosapride (4-amino-5-chloro-2-ethoxy-N-[[4-[(4-fluorophenyl) methyl]-2-morpholinyl]-methyl] benzamide) is a potent agonist at gastrointestinal 5-HT4 receptors. Mosapride is an approved drug to treat several gastric diseases. We tested the hypothesis that mosapride also stimulates 5-HT4 receptors in the heart. Mosapride increased the force of contraction and beating rate in isolated atrial preparations from mice with cardiac overexpression of human 5-HT4-serotonin receptors (5-HT4-TG). However, it is inactive in wild-type mouse hearts (WT). Mosapride was less effective and potent than serotonin in raising the force of contraction or the beating rate in 5-HT4-TG. Only in the presence of cilostamide (1 μM), a phosphodiesterase III inhibitor, mosapride, and its primary metabolite time dependently raised the force of contraction under isometric conditions in isolated paced human right atrial preparations (HAP, obtained during open heart surgery). In HAP, mosapride (10 μM) reduced serotonin-induced increases in the force of contraction. Mosapride (10 µM) shifted the concentration–response curves to serotonin in HAP to the right. These data suggest that mosapride is a partial agonist at 5-HT4-serotonin receptors in HAP.
The effects of pramipexole on motivational vigour during a saccade task: a placebo-controlled study in healthy adults
Authors: Au-Yeung Sheena K. , Halahakoon Don Chamith , Kaltenboeck Alexander , Cowen Philip , Browning Michael , Manohar Sanjay G 
Published: March 2024

AbstractMotivation allows us to energise actions when we expect reward and is reduced in depression. This effect, termed motivational vigour, has been proposed to rely on central dopamine, with dopaminergic agents showing promise in the treatment of depression. This suggests that dopaminergic agents might act to reduce depression by increasing the effects of reward or by helping energise actions. The aim of the current study was to investigate whether the dopamine agonist pramipexole enhanced motivational vigour during a rewarded saccade task. In addition, we asked whether the effects of pramipexole on vigour differ between reward contingent on performance and guaranteed reward. Healthy adult participants were randomised to receive either pramipexole (n = 19) or placebo (controls n = 18) for 18 days. The vigour of saccades was measured twice, once before the administration of study medication (Time 1) and after taking it for 12–15 days (Time 2). To separate motivation by contingency vs. reward, saccadic vigour was separately measured when (1) rewards were contingent on performance (2) delivered randomly with matched frequency, (3) when reward was guaranteed, (4) when reward was not present at all. Motivation increased response vigour, as expected. Relative to placebo, pramipexole also increased response vigour. However, there was no interaction, meaning that the effects of reward were not modulated by drug, and there was no differential drug effect on contingent vs. guaranteed rewards. The effect of pramipexole on vigour could not be explained by a speed/accuracy trade-off, nor by autonomic arousal as indexed by pupillary dilation. Chronic D2 stimulation increases general vigour, energising movements in healthy adults irrespective of extrinsic reward.
Unveiling the invisible: receivers use object weight cues for grip force planning in handover actions
Authors: Kopnarski L. , Rudisch J. , Kutz D. F. , Voelcker-Rehage C. 
Published: March 2024

AbstractHandover actions are part of our daily lives. Whether it is the milk carton at the breakfast table or tickets at the box office, we usually perform these joint actions without much conscious attention. The individual actions involved in handovers, that have already been studied intensively at the level of individual actions, are grasping, lifting, and transporting objects. Depending on the object’s properties, actors must plan their execution in order to ensure smooth and efficient object transfer. Therefore, anticipatory grip force scaling is crucial. Grip forces are planned in anticipation using weight estimates based on experience or visual cues. This study aimed to investigate whether receivers are able to correctly estimate object weight by observing the giver’s kinematics. For this purpose, handover actions were performed with 20 dyads, manipulating the participant role (giver/receiver) and varying the size and weight of the object. Due to the random presentation of the object weight and the absence of visual cues, the participants were unaware of the object weight from trial to trial. Kinematics were recorded with a motion tracking system and grip forces were recorded with customized test objects. Peak grip force rates were used as a measure of anticipated object weight. Results showed that receiver kinematics are significantly affected by object weight. The peak grip force rates showed that receivers anticipate object weight, but givers not. This supports the hypothesis that receivers obtain information about the object weight by observing giver’s kinematics and integrating this information into their own action execution.
Impact of temporal resolution on perfusion metrics, therapy decision, and radiation dose reduction in brain CT perfusion in patients with suspected stroke
Authors: Rau Alexander , Reisert Marco , Stein Thomas , Mueller-Peltzer Katharina , Rau Stephan , Bamberg Fabian , Taschner Christian A. , Urbach Horst , Kellner Elias 
Published: March 2024

Abstract
Purpose
CT perfusion of the brain is a powerful tool in stroke imaging, though the radiation dose is rather high. Several strategies for dose reduction have been proposed, including increasing the intervals between the dynamic scans. We determined the impact of temporal resolution on perfusion metrics, therapy decision, and radiation dose reduction in brain CT perfusion from a large dataset of patients with suspected stroke.

Methods
We retrospectively included 3555 perfusion scans from our clinical routine dataset. All cases were processed using the perfusion software VEOcore with a standard sampling of 1.5 s, as well as simulated reduced temporal resolution of 3.0, 4.5, and 6.0 s by leaving out respective time points. The resulting perfusion maps and calculated volumes of infarct core and mismatch were compared quantitatively. Finally, hypothetical decisions for mechanical thrombectomy following the DEFUSE-3 criteria were compared.

Results
The agreement between calculated volumes for core (ICC = 0.99, 0.99, and 0.98) and hypoperfusion (ICC = 0.99, 0.99, and 0.97) was excellent for all temporal sampling schemes. Of the 1226 cases with vascular occlusion, 14 (1%) for 3.0 s sampling, 23 (2%) for 4.5 s sampling, and 63 (5%) for 6.0 s sampling would have been treated differently if the DEFUSE-3 criteria had been applied. Reduction of temporal resolution to 3.0 s, 4.5 s, and 6.0 s reduced the radiation dose by a factor of 2, 3, or 4.

Conclusion
Reducing the temporal sampling of brain perfusion CT has only a minor impact on image quality and treatment decision, but significantly reduces the radiation dose to that of standard non-contrast CT.
Versatile ferrous oxidation–xylenol orange assay for high-throughput screening of lipoxygenase activity
Authors: Chrisnasari Ruth , Ewing Tom A. , Hilgers Roelant , van Berkel Willem J. H. , Vincken Jean-Paul , Hennebelle Marie 
Published: March 2024

Abstract
Lipoxygenases (LOXs) catalyze dioxygenation of polyunsaturated fatty acids (PUFAs) into fatty acid hydroperoxides (FAHPs), which can be further transformed into a number of value-added compounds. LOXs have garnered interest as biocatalysts for various industrial applications. Therefore, a high-throughput LOX activity assay is essential to evaluate their performance under different conditions. This study aimed to enhance the suitability of the ferrous-oxidized xylenol orange (FOX) assay for screening LOX activity across a wide pH range with different PUFAs. The narrow linear detection range of the standard FOX assay restricts its utility in screening LOX activity. To address this, the concentration of perchloric acid in the xylenol orange reagent was adjusted. The modified assay exhibited a fivefold expansion in the linear detection range for hydroperoxides and accommodated samples with pH values ranging from 3 to 10. The assay could quantify various hydroperoxide species, indicating its applicability in assessing LOX substrate preferences. Due to sensitivity to pH, buffer types, and hydroperoxide species, the assay required calibration using the respective standard compound diluted in the same buffer as the measured sample. The use of correction factors is suggested when financial constraints limit the use of FAHP standard compounds in routine LOX substrate preference analysis. FAHP quantification by the modified FOX assay aligned well with results obtained using the commonly used conjugated diene method, while offering a quicker and broader sample pH range assessment. Thus, the modified FOX assay can be used as a reliable high-throughput screening method for determining LOX activity.

Key points
• Modifying perchloric acid level in FOX reagent expands its linear detection range
• The modified FOX assay is applicable for screening LOX activity in a wide pH range
• The modified FOX assay effectively assesses substrate specificity of LOX
Characterization of the ADP-β-d-manno-heptose biosynthetic enzymes from two pathogenic Vibrio strains
Authors: Shi Zhaoxiang , Tang Yue , Wang Zhenyi , Wang Min , Zhong Zijian , Jia Jingming , Chen Yihua 
Published: March 2024

Abstract
ADP-activated β-d-manno-heptoses (ADP-β-d-manno-heptoses) are precursors for the biosynthesis of the inner core of lipopolysaccharide in Gram-negative bacteria. Recently, ADP-d-glycero-β-d-manno-heptose (ADP-d,d-manno-heptose) and its C-6′′ epimer, ADP-l-glycero-β-d-manno-heptose (ADP-l,d-manno-heptose), were identified as potent pathogen-associated molecular patterns (PAMPs) that can trigger robust innate immune responses. Although the production of ADP-d,d-manno-heptose has been studied in several different pathogenic Gram-negative bacteria, current knowledge of ADP-β-d-manno-heptose biosynthesis in Vibrio strains remains limited. Here, we characterized the biosynthetic enzymes of ADP-d,d-manno-heptose and the epimerase that converts it to ADP-l,d-manno-heptose from Vibrio cholerae (the causative agent of pandemic cholera) and Vibrio parahaemolyticus (non-cholera pathogen causing vibriosis with clinical manifestations of gastroenteritis and wound infections) in comparison with their isozymes from Escherichia coli. Moreover, we discovered that β-d-mannose 1-phosphate, but not α-d-mannose 1-phosphate, could be activated to its ADP form by the nucleotidyltransferase domains of bifunctional kinase/nucleotidyltransferases HldEVC (from V. cholerae) and HldEVP (from V. parahaemolyticus). Kinetic analyses of the nucleotidyltransferase domains of HldEVC and HldEVP together with the E. coli–derived HldEEC were thus carried out using β-d-mannose 1-phosphate as a mimic sugar substrate. Overall, our works suggest that V. cholerae and V. parahaemolyticus are capable of synthesizing ADP-β-d-manno-heptoses and lay a foundation for further physiological function explorations on manno-heptose metabolism in Vibrio strains.

Key points
• Vibrio strains adopt the same biosynthetic pathway as E. coli in synthesizing ADP-β-d-manno-heptoses.
• HldEs from two Vibrio strains and E. coli could activate β-d-mannose 1-phosphate to ADP-β-d-mannose.
• Comparable nucleotidyltransfer efficiencies were observed in the kinetic studies of HldEs.
Effect of Injectable Acellular Adipose Matrix on Soft Tissue Reconstruction in a Murine Model
Authors: Kim Jaewoo , Tran Vinh Vuong The , Hong Ki Yong , Chang Hak 
Published: March 2024

Abstract
Background
The extracellular matrix isolated from adipose tissue, known as acellular adipose matrix (AAM), represents a novel biomaterial. AAM functions as a scaffold that not only supports stem cell proliferation and differentiation but also induces adipogenesis and angiogenesis. This study aims to investigate the volumetric effects and microenvironmental changes associated with injectable AAM in comparison to conventional fat grafting.

Methods
AAM was manufactured from fresh human abdominoplasty fat using a mechanically modified method and then transformed into an injectable form. Lipoaspirate was harvested employing the Coleman technique. A weight and volume study was conducted on athymic nude mice by injecting either injectable AAM or lipoaspirate into the scalp (n=6 per group). After eight weeks, graft retention was assessed through weight measurement and volumetric analysis using micro-computed tomography (micro-CT) scanning. Histological analysis was performed using immunofluorescence staining for perilipin and CD31.

Results
Injectable AAM exhibited similar weight and volume effects in murine models. Histological analysis revealed comparable inflammatory cell presence with minimal capsule formation when compared to conventional fat grafts. Adipogenesis occurred in both AAM-injected and conventional fat graft models, with no significant difference in the blood vessel area (%) between the two.

Conclusions
In summary, injectable AAM demonstrates effectiveness comparable to conventional fat grafting concerning volume effects and tissue regeneration in soft tissue reconstruction. This promising allogeneic injectable holds the potential to serve as a safe and effective “Off-the-Shelf” alternative in both aesthetic and reconstructive clinical practices.

No Level Assigned
This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
Cheeklift With and Without Posterior Lamellar Spacer Grafts for Treatment of Lower Eyelid Retraction
Authors: Campla Christie K. , Awh Caroline , Rebollo Nicole P. , Perry Julian D. 
Published: March 2024

Abstract
Background
To compare outcomes of lower eyelid retraction repair using a subperiosteal midface lifting technique with and without posterior lamellar grafts.

Methods
Charts of patients undergoing a sub-periosteal midface lift for treatment of lower eyelid retraction using 4 techniques for posterior lamellar reconstruction were reviewed. Thirty patients were included in each of the groups: midface with hard palate graft (HPG), midface lift with acellular cadaveric graft (ADG), midface lift with retractor disinsertion (RD) and midface lift alone (NG). Measurements of distance from pupil center to lower lid margin (MRD2) and from lateral limbus to lower lid margin (MRD2limbus) were taken from pre- and postoperative photographs and compared. Secondary outcomes included rates of reoperation, major and minor complications, resolution of symptoms and keratopathy.

Results
One hundred twenty operations were assessed (n = 30 for each surgical group). The average follow-up time was 20 weeks. The median MRD2 elevation was 0.95 mm (NG), 0.85 mm (HPG), 1.59 mm (ADG) and 1.02 mm (RD). The median MRD2limbus elevation was 1.06 mm (NG), 0.92 mm (HPG), 1.45 mm (ADG) and 1.12 mm (RD). There were no significant differences in MRD2 or MRD2limbus between the 4 groups (p = 0.06 and 0.29, respectively). Reoperation rates were highest with in the hard palate graft group (33%) compared to other techniques (p = 0.0006).

Conclusions
Similar degrees of lower eyelid elevation were achieved with all the midface lifting techniques, and complication rates did not significantly differ between techniques. However, the higher reoperation rates with the use of spacer grafts suggest that a no-graft technique may be preferable.

Level of Evidence III
This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.
The Society of Information and the European Citizens’ Perception of Climate Change: Natural or Anthropological Causes
Authors: Mata Fernando , Dos-Santos Maria , Cano-Díaz Concha , Jesus Meirielly , Vaz-Velho Manuela 
Published: March 2024

Abstract
The scientific community has reached a consensus on humans’ important role as causative agents of climate change; however, branches of society are still sceptical about this. Climate change is a key issue for humanity and only the commitment to change human attitudes and lifestyles, at the global level, can be effective in its mitigation. With this purpose, it is important to convey the right message and prevent misinformation to manipulate people’s minds. The present study aims to understand the factors shaping European citizens’ thoughts on the causes of climate change. Using data from the European Social Survey 10 collected in 2022, we fitted statistical models using the people’s thoughts on causes of climate change (natural, anthropogenic or both) as dependent variables. As independent variables, we used the impact of the media through time spent on news and time spent on the internet, level of education, level of trust in scientists, awareness of online or mobile misinformation and gender. We concluded that the typical European citizen who believes in anthropogenic causes of climate change is a female, is more literate, trusts more in scientists, is younger, spends more time reading the news and has more awareness of misinformation presence in online and mobile communications.
Assessing the efficiency of the irrigation system in a horticulture field through time-lapse electrical resistivity tomography
Authors: Innocenti Agnese , Pazzi Veronica , Napoli Marco , Fanti Riccardo , Orlandini Simone 
Published: March 2024

AbstractThe characterization by means of geophysical techniques of agricultural soils subjected to continuous irrigation cycles makes it possible to study the heterogeneity of a soil and the preferential pathways of water flows without disturbing soil and plants. A better knowledge of soil heterogeneity enables optimal water resource management in terms of crop, yield, and sustainability. In this study, time-lapse monitoring using electrical resistivity tomographies (ERT) is proposed as a reliable and non-invasive technique to quantify the movement of water flows and thus the variation of soil water content during the irrigation process. ERT surveys have been conducted in melon-growing soils in southern Tuscany (Italy). Five survey campaigns have been carried out between June and August 2022, in which ERT data have been collected by taking measurements before (T0), during (T1), and after (T2) the irrigation phase. The interpretation of the ERT results provided information on the spatial and temporal distribution of water fluxes in the soil and root zone of melons during the irrigation phases. The investigation made it possible to identify the preferential pathways of infiltration of irrigation water, the points where water is absorbed by the roots, and the points where water follows a preferential pathway instead distributing itself entirely below the root growth zone. Thus, this research suggests that the ERT technique can be used to evaluate the efficiency of the irrigation system in order to achieve optimal management of the water resource, avoiding preferential flow paths that lead to less water availability for the plant.
The application of JAK inhibitors in the peri-transplantation period of hematopoietic stem cell transplantation for myelofibrosis
Authors: Wang Zerong , Jin Xuelian , Zeng Jiajia , Xiong Zilin , Chen Xinchuan 
Published: March 2024

AbstractMyelofibrosis (MF) is a myeloproliferative neoplasm (MPN) with a poor prognosis, and allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only treatment with curative potential. Ruxolitinib, a JAK1/2 inhibitor, has shown promising results in improving patients’ symptoms, overall survival, and quality of life, and can be used as a bridging therapy to HSCT that increases the proportion of transplantable patients. However, the effect of this and similar drugs on HSCT outcomes is unknown, and the reports on their efficacy and safety in the peri-transplantation period vary widely in the published literature. This paper reviews clinical data related to the use of JAK inhibitors in the peri-implantation phase of hematopoietic stem cell transplantation for primary myelofibrosis and discusses their efficacy and safety.