bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cardiac Stasis Imaging, Stroke and Silent Brain Infarcts in Patients with Non-Ischemic Dilated Cardiomyopathy
Authors: Rodríguez-González Elena , Martínez-Legazpi Pablo , González-Mansilla Ana , Espinosa M. Ángeles , Mombiela Teresa , Guzmán-De-Villoria Juan A. , Borja Maria Guadalupe , Díaz-Otero Fernando , Gómez de Antonio Rubén , Fernández-García Pilar , Fernández-Ávila Ana I , Pascual-Izquierdo Cristina , del Álamo Juan C , Bermejo Javier 
Published: March 2024

AbstractBackgroundCardioembolic stroke is one of the most devastating complications of non-ischemic dilated cardiomyopathy (NIDCM). However, in clinical trials of primary prevention, the benefits of anticoagulation were hampered by the risk of bleeding. If indices of cardiac blood stasis account for the risk of stroke, they may be useful to individualize primary prevention treatments.MethodsWe performed a cross-sectional study in patients with NIDCM and no history of atrial fibrillation (AF) from two sources: 1) a prospective enrollment of unselected patients with left ventricular (LV) ejection fraction <45% and 2) a retrospective identification of patients with a history of previous cardioembolic neurological event. The primary endpoint integrated a history of ischemic stroke, transient ischemic attack (TIA), or the presence intraventricular thrombus, or a silent brain infarction (SBI) by imaging. From echocardiography, we calculated blood flow inside the LV and its residence time (RT). The study was registered inClinicalTrials.gov(NCT03415789).ResultsOf the 89 recruited patients, 18 showed a positive primary endpoint: 9 patients had a history stroke or TIA and another 9 were diagnosed with SBIs in the brain imaging.RTperformed good to identify the primary endpoint (AUC (95% CI)= 0.75 (0.61-0.89), p= 0.001). ART> 2.21 cycles showed a sensitivity of 0.88 (0.77-1.00) and specificity of 0.70 (0.10-0.81). When accounting only for identifying a history of stroke or TIA, AUC forRTwas 0.92 (0.85-1.00) with and odds ratio= 7.2 (2.3 – 22.3) per cycle, p< 0.001.ConclusionsIn patients with NIDCM in sinus rhythm, stasis imaging derived from echocardiographyis is closely related to the burden of stroke. Stasis imaging may be useful to address stroke risk in patients with systolic dysfunction.
A human commons cell atlas reveals cell type specificity for OAS1 isoforms
Authors: Galvez-Merchán Ángel , Booeshaghi A. Sina , Pachter Lior 
Published: March 2024

AbstractWe describe an open source Human Commons Cell Atlas comprising 2.9 million cells across 27 tissues that can be easily updated and that is structured to facilitate custom analyses. To showcase the flexibility of the atlas, we demonstrate that it can be used to study isoforms of genes at cell resolution. In particular, we study cell type specificity of isoforms of OAS1, which has been shown to offer SARS-CoV-2 protection in certain individuals that display higher expression of the p46 isoform. Using our commons cell atlas we localize the OAS1 p44b isoform to the testis, and find that it is specific to round and elongating spermatids. By virtue of enabling customized analyses via a modular and dynamic atlas structure, the commons cell atlas should be useful for exploratory analyses that are intractable within the rigid framework of current gene-centric cell atlases.
Translocation of YopJ family effector proteins through the VirB/VirD4 T4SS ofBartonella
Authors: Fromm Katja , Ortelli Monica , Boegli Alexandra , Dehio Christoph 
Published: March 2024

AbstractThe evolutionary conserved YopJ family comprises numerous type-III-secretion system (T3SS) effectors of diverse mammalian and plant pathogens that acetylate host proteins to dampen immune responses. Acetylation is mediated by a central acetyltransferase domain that is flanked by conserved regulatory sequences, while a non-conserved N-terminal extension encodes the T3SS-specific translocation signal.Bartonellaspp. are facultative-intracellular pathogens causing intra-erythrocytic bacteremia in their mammalian reservoirs and diverse disease manifestations in incidentally infected humans. Bartonellae do not encode a T3SS, but most species possess a type-IV-secretion system (T4SS) to translocateBartonellaeffector proteins (Beps) into host cells. Here we report that the YopJ homologs present in Bartonellae species represent genuine T4SS effectors. Like YopJ family T3SS effectors of mammalian pathogens, the ‘BartonellaYopJ-like effector A’ (ByeA) ofBartonella tayloriialso targets MAP kinase signaling to dampen pro-inflammatory responses, however, translocation depends on a functional T4SS. A split-NanoLuc luciferase-based translocation assay identified sequences required for T4SS-dependent translocation in conserved regulatory regions at the C-terminus and proximal to the N-terminus of ByeA. The T3SS effectors YopP fromYersinia enterocoliticaand AvrA fromSalmonellaTyphimurium were also translocated via theBartonellaT4SS, while ByeA was not translocated via theYersiniaT3SS. Our data suggest that YopJ family T3SS effectors may have evolved from an ancestral T4SS effector, such as ByeA ofBartonella. In this evolutionary scenario, the signal for T4SS-dependent translocation encoded by N- and C-terminal sequences remained functional in the derived T3SS effectors due to the essential role these sequences coincidentally play in regulating acetyltransferase activity.Significance StatementBacterial pathogens use diverse secretion systems to translocate effector proteins into eukaryotic host cells. Evolutionary successful translocation systems and effector proteins have been acquired by many plant and animal pathogens via horizontal gene transfer. The YopJ family comprises numerous T3SS effectors that share a unique acetyltransferase activity that interferes with various host cell functions. Our study revealed that YopJ homologs in the pathogenBartonellaare genuine T4SS effectors and implies an evolutionary scenario in which T3SS-dependent YopJ family effectors may have evolved from such an ancestral T4SS effector by fusion of an N-terminal type-III-secretion signal. Such switches in secretion system specificity of host-targeted effectors may represent an underappreciated phenomenon in effector evolution.
Oligonucleotide Library Assisted Sequence Mining Reveals Promoter Sequences With Distinct Temporal Expression Dynamics For Applications InCurvibactersp. AEP1-3
Authors: Mager Maurice , Becker Lukas , Schulten Nina , Fraune Sebastian , Axmann Ilka M. 
Published: March 2024

AbstractTheß-proteobacterialspeciesCurvibactersp. AEP1-3 is a model organism for the study of symbiotic interactions as it is the most abundant bacterial colonizer of the basal metazoanHydra vulgaris. Yet, genetic tools forCurvibacterare still in an infancy: few promoters have been characterized forCurvibacter. Here we employ an oligonucleotide based strategy to find potential expression systems derived from the genome ofCurvibacter. Potential promoters were systematically mined from the genome in silico. The sequences were cloned as a mixed library into a mCherry reporter gene expression vector and single positive candidates were selected through Flow Cytometry based sorting to be further analyzed through bulk measurements. From 500 candidate sequences, 25 were identified as active promoters of varying expression strength levels. Bulk measurements revealed unique activity profiles for these sequences across growth phases. The expression levels of these promoters ranged over two orders of magnitudes and showed distinct temporal expression dynamics over the growth phases: while 3 sequences showed higher expression levels in the exponential phase than in the stationary phase, we found 12 sequences saturating expression during stationary phase and 10 that showed little discrimination between growth phases. From our library, promoters the genes encoding for DnaK, RpsL and an AHL synthase stood out as the most interesting candidates as their expression profiles fit a variety of applications. Examining the expression levels of successful candidates in relation to RNAseq read counts revealed only weak correlation between the two datasets. This underscores the importance of employing comprehensive high-throughput strategies when establishing expression systems for newly introduced model organisms.
Defining the constituents and functions of a lipid-based jumbo phage compartment
Authors: Mozumdar Deepto , Fossati Andrea , Stevenson Erica , Guan Jingwen , Nieweglowska Eliza , Rao Sanjana , Agard David , Swaney Danielle L. , Bondy-Denomy Joseph 
Published: March 2024

ABSTRACTViruses are vulnerable to cellular defenses at the start of the infection when a single copy genome has not yet initiatedde novoviral protein synthesis. A family of jumbo phages related to phage ΦKZ, which infectsPseudomonas aeruginosa,uses a protein-based phage nucleus to protect the DNA during middle and late stages of infection, but how it is protected prior to phage nucleus assembly is unclear. To reveal the environment surrounding injected phage DNA, we determine which proteins interact with phage proteins injected with the phage genome. Here, we surprisingly identify host proteins related to membrane and lipid biology co-purifying with the injected phage protein. Staining of infected cells with lipophilic dyes revealed a clustering of host lipids co-localized with phage DNA and protein in an early phage infection (EPI) vesicle. Early gene expression is induced by the co-injected virion RNAP (vRNAP), with specific early proteins then associating with the EPI vesicle and ribosomes, likely to facilitate localized translation. As the infection progresses, the phage genome separates from the EPI vesicle and vRNAP, moving into the nascent proteinaceous phage nucleus. Enzymes involved in DNA replication and CRISPR/restriction immune nucleases notably do not localize to the EPI vesicle, supporting that DNA is not exposed at this early time point. We therefore propose that the EPI vesicle is rapidly constructed together with injected phage proteins, phage DNA, host lipids, and host membrane proteins to enable genome protection, early transcription, localized translation, and to ensure the faithful transfer of the single copy phage genome to the proteinaceous nucleus.
Clinical Retrospective Analysis: Higher Rates of Gene Mutations in the Microsatellite Stable (MSS) Population of Colorectal Cancer
Authors: Liu Xionglin , Zhou Yuan , Mao Wei , Pei Haiping 
Published: March 2024

AbstractWith the increasing incidence of colorectal cancer, understanding different subgroups, such as the microsatellite stable (MSS) population, is crucial. Our study aimed to explore the unique characteristics and underlying genetic and epigenetic features of the microsatellite stable (MSS) population within colorectal cancer. Using 325 samples, we compared MSS with other microsatellite statuses on more than 50 clinical indicators. A significantly different overall gene mutation rate was observed in the MSS group compared to the other groups, especially in P53 mutations. No notable changes were found in epigenetics. The results suggest that MSS colorectal cancer cases are more likely to have gene mutations than other subgroups. We also found that the N stage was lower in the MSI-H group. These findings provide crucial insights that can guide future drug development and treatment plans for the MSS population.
Compartmentalized localization of perinuclear proteins within germ granules inC. elegans
Authors: Huang Xiaona , Feng Xuezhu , Yan Yong-hong , Xu Demin , Wang Ke , Zhu Chengming , Dong Meng-qiu , Huang Xinya , Guang Shouhong , Chen Xiangyang 
Published: March 2024

AbstractBiomolecular condensates are nonmembrane-enclosed organelles that play important roles in distinct biological processes. Germ granules are RNA-rich condensates that are often docked on the cytoplasmic surface of germline nuclei and exist in a variety of organisms. TheC. elegansgerm granule is a condensate that is subcompartmentalized into at least 6 distinct regions, including the P granule, Z granule, E granule, Mutator foci, SIMR foci and P-body. Although a number of perinuclear proteins have been found to be enriched in germ granules, their precise localization within the subcompartments of germ granules is still unclear. Here, we systematically labeled perinuclear localized proteins with fluorescent proteins via CRISPR/Cas9 technology and further explored the perinuclear localization of these proteins. Using this nematode strain library, we identified a series of proteins localized in Z or E granules. We found that proteins that participate in distinct piRNA processing steps were localized in particular subcompartments, including the P-, Z-, and E-granules. We further identified a novel subcompartment, the D granule, which was enriched between the P granule and the nuclear pore complex. Finally, we analyzed the perinuclear localization of these germ granule subcompartments inmip-1/eggd-1mutants and found that the LOTUS domain protein MIP-1/EDDG-1 was required for the establishment of the multiphase architecture of germ granules. Overall, our work revealed germ granule architecture and redefined the localization of perinuclear proteins within germ granules. Additionally, the library of genetically modified nematode strains expressing peri-nuclear proteins labeled with fluorescent tags will facilitate research on germ granules inC. elegans.
Brownian Electric Bubble Quasiparticles
Authors: Aramberri Hugo , Íñiguez-González Jorge 
Published: March 2024

Recent works on electric bubbles (including the experimental demonstration of electric skyrmions) constitute a breakthrough akin to the discovery of magnetic skyrmions some 15 years ago. So far research has focused on obtaining and visualizing these objects, which often appear to be immobile (pinned) in experiments. Thus, critical aspects of magnetic skyrmions—e.g., their quasiparticle nature, Brownian motion—remain unexplored (unproven) for electric bubbles. Here we use predictive atomistic simulations to investigate the basic dynamical properties of these objects in pinning-free model systems. We show that it is possible to find regimes where the electric bubbles can present long lifetimes (∼ns) despite being relatively small (diameter <2  nm). Additionally, we find that they can display stochastic dynamics with large and highly tunable diffusion constants. We thus establish the quasiparticle nature of electric bubbles and put them forward for the physical effects and applications (e.g., in token-based probabilistic computing) considered for magnetic skyrmions.

Published by the American Physical Society
2024
Does inclusive leadership influence employee innovation? A multilevel investigation
Authors: Bao Ping , Liao Zhongju , Li Chao 
Published: March 2024

PurposeThe purpose of this research is to investigate the cross-level effects and mechanisms of inclusive leadership on employee innovation in team contexts, and further explore the boundary conditions of inclusive leadership.Design/methodology/approachThis study collected data from 237 leader-member dyads in 60 teams of Chinese firms. The research utilized multilevel linear models and multilevel structural equation models in the R language to test the hypothesized model.FindingsThe findings suggest that inclusive leadership has a positive impact on both employee incremental and radical innovation. Team psychological safety and employee role breadth self-efficacy mediate the effects. Employee risk avoidance propensity negatively moderates the mediating role of role breadth self-efficacy in the relationship between inclusive leadership and incremental innovation.Practical implicationsLeaders should pay attention to team psychological safety, employee role breadth self-efficacy and employee individual risk avoidance propensity that influence employee innovation to maximize the effectiveness of inclusive leadership.Originality/valueThis research expanded the level of analysis from individual to team, exploring cross-level effects and mechanisms of inclusive leadership on employee innovation in team contexts, and clarified the effectiveness conditions of inclusive leadership.
Digital entrepreneurship intention and digital entrepreneurship behavior: the mediating role of managing learning and entrepreneurship education
Authors: Li Sihui , Bu Yajing , Zhang Zeyuan , Huang Yangjie 
Published: March 2024

PurposeWith the development of the digital economy, digital entrepreneurship has become increasingly popular. For college students preparing for digital entrepreneurship, it is necessary to cope with the uncertainty of the start-up process through meaningful managing learning and continuous entrepreneurship education. The purpose of this study is to examine the relationship between Chinese college students' digital entrepreneurship intention and digital entrepreneurship behavior, as well as the role of managing learning and entrepreneurship education in this relationship.Design/methodology/approachBased on the existing literature, this study established the digital entrepreneurship hypothesis model and investigated the digital entrepreneurship behavior of college students.FindingsThe results showed that managing learning and entrepreneurship education can promote the transformation of the digital entrepreneurship intention to digital entrepreneurship behavior. Managing learning and entrepreneurship education played a driving role in the transformation of the digital entrepreneurship intention to digital entrepreneurship behavior.Originality/valueThis study explored the complex mechanism of the relationship between digital entrepreneurship intention and digital entrepreneurship behavior among Chinese college students. Based on survey data from 235 college students in China, the empirical results supported theoretical research hypotheses on the relationship between college students and digital entrepreneurship intention, digital entrepreneurship behavior, managing learning and entrepreneurship education.
Framing a feminist phenomenological inquiry into the lived experiences of women entrepreneurs
Authors: Oliveira Edicleia , Basini Serge , Cooney Thomas M. 
Published: March 2024

PurposeThis article aims to explore the potential of feminist phenomenology as a conceptual framework for advancing women’s entrepreneurship research and the suitability of interpretative phenomenological analysis (IPA) to the proposed framework.Design/methodology/approachThe article critically examines the current state of women’s entrepreneurship research regarding the institutional context and highlights the benefits of a shift towards feminist phenomenology.FindingsThe prevailing disembodied and gender-neutral portrayal of entrepreneurship has resulted in an equivocal understanding of women’s entrepreneurship and perpetuated a male-biased discourse within research and practice. By adopting a feminist phenomenological approach, this article argues for the importance of considering the ontological dimensions of lived experiences of situatedness, intersubjectivity, intentionality and temporality in analysing women entrepreneurs’ agency within gendered institutional contexts. It also demonstrates that feminist phenomenology could broaden the current scope of IPA regarding the embodied dimension of language.Research limitations/implicationsThe adoption of feminist phenomenology and IPA presents new avenues for research that go beyond the traditional cognitive approach in entrepreneurship, contributing to theory and practice. The proposed conceptual framework also has some limitations that provide opportunities for future research, such as a phenomenological intersectional approach and arts-based methods.Originality/valueThe article contributes to a new research agenda in women’s entrepreneurship research by offering a feminist phenomenological framework that focuses on the embodied dimension of entrepreneurship through the integration of IPA and conceptual metaphor theory (CMT).
Non-communicable diseases and the prospects of integrated behavioral health in India: current status and future directions
Authors: Padma Sri Lekha P. , Abdul Azeez E.P. , O'Donnell Ronald R. 
Published: March 2024

PurposeContextual to the recognition of the complex interplay between health and behavioral aspects, integrated behavioral health (IBH) has emerged. Although this model is becoming popular in the Western world, its presence in the global context is not promising. This paper aims to explore the need for IBH in India and address its barriers to implementation and possible solutions.Design/methodology/approachWe analyzed the case of IBH and its potential implications for India using the current evidence base, authors' reflections and experience of implementing similar programs.FindingsThis paper identifies contextual factors, including increased instances of non-communicable diseases and psychosocial and cultural determinants of health, that necessitate the implementation of IBH programs in India. The key features of different IBH models and their applicability are outlined. The current status of IBH and potential challenges in implementation in India in terms of human resources and other factors are delineated. We also discuss the potential models for implementing IBH in India.Originality/valueIntegrating behavioral health in primary care is considered an effective and sustainable model to promote health and well-being across various target populations. Towards this end, this paper is the first to discuss the contextual factors of IBH in India. It is a significant addition to the knowledge base on IBH and its possible implementation barriers and strategies in low- and middle-income countries.
Towards a future conceptualization of destination resilience: exploring the role of actors, agency and resilience narratives
Authors: Posch Eva , Eckert Elena , Thiebes Benni 
Published: March 2024

PurposeDespite the widespread use and application of resilience, much uncertainty about the conceptualization and operationalization in the context of tourism destinations still exists. The purpose of this paper is to provide a conceptual elaboration on destination resilience and to introduce a model for an improved understanding of the concept.Design/methodology/approachTaking a conceptual research approach, this paper seeks to untangle the fuzziness surrounding the destination and resilience concept by providing a new interpretation that synthesizes theories and concepts from various academic disciplines. It analyses the current debate to derive theoretic baselines and conceptual elements that subsequently inform the development of a new “Destination Resilience Model”.FindingsThe contribution advances the debate by proposing three key themes for future resilience conceptualizations: (1) the value of an actor-centered and agency-based resilience perspective; (2) the importance of the dynamic nature of resilience and the (mis)use of measurement approaches; (3) the adoption of a dualistic resilience perspective distinguishing specified and general resilience. Building on these propositions, we introduce a conceptual model that innovatively links elements central to the concepts of destination and risk and combines different narratives of resilience.Originality/valueThe contribution advances the debate surrounding destination resilience by critically examining the conceptualization and operationalization of destination resilience within previous research and by subsequently proposing a “Destination Resilience Model” that picks up central element of the three new frontiers identified in the conceptually driven review. The innovative integration strengthens the comprehension of the resilience concept at destination level and supports building future capacities to manage immediate adverse impacts as well as novel and systemic risks.