bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pubertal maturation modifies the regulation of insulin‐like growth factor‐I receptor signaling by estradiol in the rat prefrontal cortex
Authors: Sanz Amaya , Carrero Paloma , Carrero Paloma , Pernía Olga , Garcia‐Segura Luis M. 
Published: April 2008

AbstractThe transition from adolescence to adulthood is accompanied by substantial plastic modifications in the cerebral cortex, including changes in the growth and retraction of neuronal processes and in the rate of synaptic formation and neuronal loss. Some of these plastic changes are prevented in female rats by prepubertal ovariectomy. The ovarian hormone estradiol modulates neuronal differentiation and survival and these effects are in part mediated by the interaction with insulin‐like growth factor‐I (IGF‐I). In this study, we have explored whether the activation by estradiol of some components of IGF‐I receptor signaling is altered in the prefrontal cortex during puberty. Estradiol administration to rats ovariectomized after puberty resulted, 24 h after the hormonal administration, in a sustained phosphorylation of Akt and glycogen synthase kinase 3β in the prefrontal cortex. However, this hormonal effect was not observed in animals ovariectomized before puberty. These findings suggest that during pubertal maturation there is a programming by ovarian hormones of the future regulatory actions of estradiol on IGF‐I receptor signaling in the prefrontal cortex. The modification in the regulation of IGF‐I receptor signaling by estradiol during pubertal maturation may have implications for the developmental changes occurring in the prefrontal cortex in the transition from adolescence to adulthood. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008.
Locomotor control by the central complex in Drosophila—An analysis of the tay bridge mutant
Authors: Poeck Burkhard , Triphan Tilman , Neuser Kirsa , Strauss Roland 
Published: April 2008

AbstractSeveral aspects of locomotor control have been ascribed to the central complex of the insect brain; however, the role of distinct substructures of this complex is not well known. The tay bridge1 (tay1) mutant of Drosophila melanogaster was originally isolated on the basis of reduced walking speed and activity. In addition, tay1 is defective in the compensation of rotatory stimuli during walking and histologically, tay1 causes a mid‐sagittal constriction of the protocerebral bridge, a constituent of the central complex. Cloning of the tay gene revealed that it encodes a novel protein with no significant homology to any known protein. To associate the behavioral phenotypes with the anatomical defect in the protocerebral bridge, we used different driver lines to express the tay cDNA in various neuronal subpopulations of the central brain in tay1‐mutant flies. These experiments showed an association of the aberrant walking speed and activity with the structural defect in the protocerebral bridge. In contrast, the compensation of rotatory stimuli during walking was rescued without a restoration of the protocerebral bridge. The results of our differential rescue approach are supported by neuronal silencing experiments using conditional tetanus toxin expression in the same subset of neurons. These findings show for the first time that the walking speed and activity is controlled by different substructures of the central brain than the compensatory locomotion for rotatory stimuli. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008.
Targeting TARP, a novel breast and prostate tumor‐associated antigen, with T cell receptor‐like human recombinant antibodies
Authors: Epel Malka , Carmi Irit , Soueid‐Baumgarten Sharon , Oh SangKon , Bera Tapan , Pastan Ira , Berzofsky Jay , Reiter Yoram 
Published: April 2008

AbstractMHC class I molecules are important components of immune surveillance. There are no available methods to directly visualize and determine the quantity and distribution of MHC/peptide complexes on individual cells or to detect such complexes on antigen‐presenting cells in tissues. MHC‐restricted recombinant antibodies with the same specificity of T cell receptors (TCR) may become a valuable tool to address these questions. They may also serve as valuable targeting molecules that mimic the specificity of cytotoxic T cells. We isolated by phage display a panel of human recombinant Fab antibodies with peptide‐specific, MHC‐restricted TCR‐like reactivity directed toward HLA‐A2‐restricted T cell epitopes derived from a novel antigen termed TCRγ alternative reading frame protein (TARP) which is expressed on prostate and breast cancer cells. We have characterized one of these recombinant antibodies and demonstrated its capacity to directly detect specific HLA‐A2/TARP T cell epitopes on antigen‐presenting cells that have complexes formed by naturally occurring active intracellular processing of the antigen, as well as on the surface of tumor cells. Moreover, by genetic fusion we armed the TCR‐like antibody with a potent toxin and demonstrated that it can serve as a targeting moiety killing tumor cells in a peptide‐specific, MHC‐restricted manner similar to cytotoxic T lymphocytes.
Acrolein Diethyl Acetal: A Three‐Carbon Homologating Reagent for the Synthesis of β‐Arylpropanoates and Cinnamaldehydes by Heck Reaction Catalyzed by a Kaiser Oxime Resin Derived Palladacycle
Authors: Alacid Emilio , Nájera Carmen 
Published: April 2008

AbstractA polymer palladacycle derived from Kaiser oxime resin was used as a source of palladium(0) in the chemoselective Heck reaction of acrolein diethyl acetal with aryl halides under ligand‐free conditions. The use of typical Heck conditions afforded 3‐arylpropionic esters, and the process can be directed to the synthesis of cinnamaldehydes under Cacchi conditions. These processes take place with rather low loading of the catalyst, which can be recovered by simple filtration and reused for at least five runs without competitive dehalogenation. This is the first time that a supported palladium complex has been reused under Cacchi conditions. ICP‐OES analyses of the Pd content of the crude products in both transformations indicated lower leaching for the esters than for the aldehydes in the range up to 0.08 ppm for the esters and 0.8 ppm for the aldehydes.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Tripodal Cadmium Complex and Macrocyclic Ligand Based Sensors for Phosphate Ion Determination in Environmental Samples
Authors: Kumar Ashok , Mehtab Sameena , Singh Udai P. , Aggarwal Vaibhave , Singh Jitendra 
Published: May 2008

AbstractTripodal cadmium complex of hydrotris(3‐phenyl‐5‐methylpyrazolyl)borate (I1) and macrocyclic ligand 5,7 : 12,14 : 19,21 : 26,28‐Bzo4‐[28]‐5,13,19,27‐tetraene‐8,11,22,25‐N4–1,4,15,18‐O4 (I2), have been synthesized and characterized by IR, 1H NMR, Mass and elemental analysis. Spectroscopic investigations indicate high affinity of these receptors for dihydrogen phosphate ion. Polyvinyl chloride (PVC) based membranes of (I1) and (I2) using hexadecyl trimethylammonium bromide (HTAB) as cation discriminator and dibutylpthalate (DBP), tributyl phosphate (TBP), dioctylsebacate (DOS), and o‐nitrophenyloctyl ether (o‐NPOE), as plasticizing solvent mediators were prepared and investigated as H2PO$\rm{ {_{4}^{-}}}$ selective sensors. The best performance was shown by the membrane of composition (w/w) (I2) (5%):PVC (31%) : DBP (61.5%):HTAB (2.5%). This sensor works well over a wide concentration range 2.1×10−7 to 1.0×10−2 M with Nernstian compliance (59.0 mV decade−1 of activity) with a fast response time of 14 s and showed good selectivity for dihydrogen phosphate ion over a number of anions. The sensor exhibits good reproducibility (SD±0.3 mV) and could be used successfully for the determination of phosphate in soil water samples.
Comprehensive proteome analysis of mouse liver by ampholyte‐free liquid‐phase isoelectric focusing
Authors: Zhong Hua , Yun Dong , Zhang Chen , Yang Pengyuan , Fan Huizhi , He Fuchu 
Published: April 2008

AbstractIn this study, ampholyte‐free liquid‐phase IEF (LIEF) was combined with narrow pH range 2‐DE and SDS‐PAGE RP‐HPLC for comprehensive analysis of mouse liver proteome. Because LIEF prefractionation was able to reduce the complexity of the sample and enhance the loading capacity of IEF strips, the number of visible protein spots on subsequent 2‐DE gels was significantly increased. A total of 6271 protein spots were detected after integrating five narrow pH range 2‐DE gels following LIEF prefractionation into a single virtual 2‐DE gel. Furthermore, the pH 3–5 LIEF fraction and the unfractionated sample were separated by pH 3–6 2‐DE and identified by MALDI‐TOF/TOF MS, respectively. In parallel, the pH 3–5 LIEF fraction was also analyzed by SDS‐PAGE RP‐HPLC MS/MS. LIEF‐2‐DE and LIEF‐HPLC could obviously improve the separation efficiency and the confidence of protein identification, which identified a higher number of low‐abundance proteins and proteins with extreme physicochemical characteristics or post‐translational modifications compared to conventional 2‐DE method. Furthermore, there were 207 proteins newly identified in mouse liver in comparison with previously reported large‐scale datasets. It was observed that the combination of LIEF‐2‐DE and LIEF‐HPLC was effective in promoting MS‐based liver proteome profiling and could be applied on similar complex tissue samples.
Performance evaluation of submerged membrane bioreactor pilot units for refinery wastewater treatment
Authors: Torres Ana Paula R. , Santiago Vânia M.J. , Borges Cristiano P. 
Published: May 2008

AbstractA comparative study was conducted by using membrane bioreactor (MBR) pilot units at a Brazilian petroleum refinery (Betim/MG) in order to make possible the implement of this technology at PETROBRAS. This study is part of the water and wastewater reuse project conducted by Petrobras Research & Development Center (CENPES). All pilot units evaluated have the submerged membrane configuration with microfiltration polymeric membranes. One of them operates with flat sheet membranes, while the other uses hollow fiber membranes. The units have hydraulic capacity of 0.5–2.0 m3/h, and 60–70 m2 of installed membrane area, permitting to operate with a permeate flux of 7–30 L/m2 h. The pilot units evaluation was carried out by monitoring the main biological and membrane process parameters. Results obtained from the study showed that all the MBR systems consistently produced effluent with turbidity <1 NTU, BOD5 <5 mg/L, and NH3N < 3 mg/L, corresponding to 98, 97, and 96% removal, respectively. These results allow considering the MBR as an appropriate technology for pretreatment of a membrane water desalination unit for steam production or generation of high quality water for industrial reuse as cooling tower make up. © 2008 American Institute of Chemical Engineers Environ Prog, 2008.
Microbial‐sponge and microbial‐metazoan buildups in the Late Viséan basin‐fill sequence of the Jerada Massif (Carboniferous, NE Morocco)
Authors: Aretz Markus , Herbig Hans‐Georg 
Published: April 2008

AbstractThree distinctive Late Viséan buildup intervals are differentiated on the southern limb of the Jerada Synclinorium. The oldest buildups (BI‐1) are preserved as olistoliths in the Oued El Koriche Formation (Fm). They were located on a southern platform prior to reworking. The younger Koudiat Es‐Senn Fm contains two autochthonous buildup intervals (BI‐2 to BI‐3). BI‐1 and BI‐2 buildups are large microbial‐sponge buildups, BI‐3 are small microbial‐metazoan buildups in the capping bed succession above BI‐2. All buildups are characterized by a rigid non‐skeletal microbial framework. Growth of the largest BI‐2 buildups started in aphotic to dysphotic conditions in a water depth significantly less than 100 m and continued into agitated shallow‐water. Smaller BI‐2 buildups and all BI‐3 buildups are encased in grainy shallow‐water carbonates. In contrast to a mostly homogeneous microbial‐sponge facies below, the uppermost part of the BI‐2 buildups and all BI‐3 buildups consist of heterogeneous boundstones with differing frame builders. BI‐1 to BI‐3 buildups represent different stages within a Viséan basin‐fill sequence. Carbonate sedimentation ended with the deposition of peritidal oncolitic floatstones below the unconformably overlying Namurian shales. The Jerada buildups have many features commonly associated with ‘reefs’. Comparison with contemporaneous buildups shows considerable similarities to buildups from Algeria, the Eastern Moroccan Anti‐Atlas and the British Isles. Copyright © 2008 John Wiley & Sons, Ltd.
Suicidal thoughts among elderly Taiwanese aboriginal women
Authors: Chen Cheng‐Sheng , Yang Mei‐Sang , Yang Ming‐Jen , Chang Shun‐Jen , Chueh Ke‐Hsin , Su Yi‐Ching , Yu Ching‐Yun , Cheng Tai‐Chun 
Published: April 2008

AbstractObjectivesThe aims of this study were to investigate prevalence of suicidal thoughts among a population of elderly aboriginal women in Taiwan over a 1‐month period and to examine the risk factors for suicidal thinking in terms of individual (self‐perceived health, disability and financial difficulty), family (marital discord) and social (medical accessibility) aspects. The mediating effects of depression on the above risk factors were also investigated. Furthermore, we examined the buffer effect on suicidal ideation of emotional social support for dealing with marital discord.MethodsOne thousand three hundred and forty‐seven elderly Taiwanese aboriginal women were enrolled. Suicide thoughts within the past month, demographic data, adverse life events, emotional social support and depressive state were assessed. The 1‐month prevalence of suicide thoughts was calculated. The risks of suicide thought based on individual, family and community aspects were estimated.ResultsThe 1‐month prevalence of suicidal thoughts among the community‐dwelling aboriginal elderly women was 17.8%. Those subjects with poorer self‐perceived health, difficulty in accessing medical resources, or experiencing marital discord were at higher risk of having suicidal thoughts. After controlling for depression, the odds ratio of self‐perceived health and marital discord remained statistically significant. The odds ratio of interaction of marital discord and emotional social support was 0.41.ConclusionSuicidal thoughts are common among the community‐dwelling aboriginal elderly women in Taiwan. Risk factors for suicidal thoughts comprise individual (depression and physical condition), family (marital discord) and community (medical resources) aspects. Better emotional social support can effectively buffer the effect of marital discord. Copyright © 2008 John Wiley & Sons, Ltd.
Avalanche dynamics of human brain oscillations: Relation to critical branching processes and temporal correlations
Authors: Poil Simon‐Shlomo , van Ooyen Arjen , Linkenkaer‐Hansen Klaus 
Published: May 2008

AbstractHuman brain oscillations fluctuate erratically in amplitude during rest and exhibit power‐law decay of temporal correlations. It has been suggested that this dynamics reflects self‐organized activity near a critical state. In this framework, oscillation bursts may be interpreted as neuronal avalanches propagating in a network with a critical branching ratio. However, a direct comparison of the temporal structure of ongoing oscillations with that of activity propagation in a model network with critical connectivity has never been made. Here, we simulate branching processes and characterize the activity propagation in terms of avalanche life‐time distributions and temporal correlations. An equivalent analysis is introduced for characterizing ongoing oscillations in the alpha‐frequency band recorded with magnetoencephalography (MEG) during rest. We found that models with a branching ratio near the critical value of one exhibited power‐law scaling in life‐time distributions with similar scaling exponents as observed in the MEG data. The models reproduced qualitatively the power‐law decay of temporal correlations in the human data; however, the correlations in the model appeared on time scales only up to the longest avalanche, whereas human data indicate persistence of correlations on time scales corresponding to several burst events. Our results support the idea that neuronal networks generating ongoing alpha oscillations during rest operate near a critical state, but also suggest that factors not included in the simple classical branching process are needed to account for the complex temporal structure of ongoing oscillations during rest on time scales longer than the duration of individual oscillation bursts. Hum Brain Mapp 2008. © 2008 Wiley‐Liss, Inc.
Hippocampal atrophy is the critical brain change in patients with hypoxic amnesia
Authors: Di Paola M. , Caltagirone C. , Fadda L. , Sabatini U. , Serra L. , Carlesimo G.A. 
Published: April 2008

AbstractAnoxia is considered a good model for studying amnesia. However, not all individuals who experience anoxic events develop memory problems. Moreover, the question still remains about whether, after anoxia, damage is limited to the hippocampus in patients with amnesia and without other significant cognitive deficits. Here we investigated brain damage in a selected sample of adults affected exclusively by an amnesic syndrome after an anoxic episode. The cerebral MR images of these patients were submitted to visual inspection, volumetric measurements of the mesial temporal structures following manual segmentation, and to Voxel‐Based Morphometry of the whole brain. We studied five anoxic patients and thirty‐three well‐matched healthy subjects. Our aim was to: (a) quantify regional atrophic changes associated with chronic anoxic damage compared to control subjects (Group Comparison Analysis); (b) identify regions of common abnormality across all patients (Conjunction Analysis in the VBM); (c) investigate whether measures of regional volume reduction correlated with neuropsychological memory scores; (d) compare the results obtained with visual inspection and ROI analyses with those obtained with VBM. We found that anoxic patients presented a significant reduction of gray matter volume in the hippocampus bilaterally compared to healthy subjects. The only common atrophic region across all patients was the hippocampus bilaterally. Correlation analysis showed only a trend between the Prose immediate free recall test and the left hippocampus. Our findings confirm that the hippocampus is very sensitive to damage stemming from anoxia. Patients with hypoxic amnesia may present damage in other brain regions, but only hippocampal atrophy is common in all of them. © 2008 Wiley‐Liss, Inc.
Palmitate increases superoxide production through mitochondrial electron transport chain and NADPH oxidase activity in skeletal muscle cells
Authors: Lambertucci Rafael Herling , Hirabara Sandro Massao , Silveira Leonardo dos Reis , Levada‐Pires Adriana Cristina , Curi Rui , Pithon‐Curi Tania Cristina 
Published: April 2008

AbstractThe effect of unbound palmitic acid (PA) at plasma physiological concentration range on reactive oxygen species (ROS) production by cultured rat skeletal muscle cells was investigated. The participation of the main sites of ROS production was also examined. Production of ROS was evaluated by cytochrome c reduction and dihydroethidium oxidation assays. PA increased ROS production after 1 h incubation. A xanthine oxidase inhibitor did not change PA‐induced ROS production. However, the treatment with a mitochondrial uncoupler and mitochondrial complex III inhibitor decreased superoxide production induced by PA. The importance of mitochondria was also evaluated in 1 h incubated rat soleus and extensor digitorum longus (EDL) muscles. Soleus muscle, which has a greater number of mitochondria than EDL, showed a higher superoxide production induced by PA. These results indicate that mitochondrial electron transport chain is an important contributor for superoxide formation induced by PA in skeletal muscle. Results obtained with etomoxir and bromopalmitate treatment indicate that PA has to be oxidized to raise ROS production. A partial inhibition of superoxide formation induced by PA was observed by treatment with diphenylene iodonium, an inhibitor of NADPH oxidase. The participation of this enzyme complex was confirmed through an increase of p47phox phosphorylation after treatment with PA. J. Cell. Physiol. 216: 796–804, 2008, © 2008 Wiley‐Liss, Inc.
Elevated fibroblast growth factor‐2 increases tumor necrosis factor‐α induced endothelial cell death in high glucose
Authors: Clyne Alisa Morss , Zhu Han , Edelman Elazer R. 
Published: April 2008

AbstractGlucose and tumor necrosis factor‐α (TNFα) concentrations are elevated in diabetes. Both of these factors correlate with diabetic vasculopathy and endothelial cell apoptosis, yet their combined effects have not been measured. We have previously shown that the angiogenic growth factor fibroblast growth factor‐2 (FGF‐2), which is generally protective against endothelial cell death, is similarly elevated in high glucose conditions. We therefore investigated the effect of TNFα on endothelial cell death under normal and elevated glucose conditions, with a particular focus on FGF‐2. Porcine aortic endothelial cells were cultured in 5 and 30 mM glucose and stimulated with TNFα, together with FGF‐2 or a neutralizing FGF‐2 antibody. Cell death was measured via cell counts or an annexin apoptotic assay, and cell cycle phase was determined by propidium iodide labeling. TNFα‐induced endothelial cell death increased for cells in high glucose, and cell death was enhanced with increasing FGF‐2 exposure and negated by a neutralizing FGF‐2 antibody. Endothelial cells were most susceptible to TNFα‐induced cell death when stimulated with FGF‐2 18 h prior to TNFα, corresponding to cell entry into S phase of the proliferative cycle. The FGF‐2 associated increase in TNFα‐induced cell death was negated by blocking cell entry into S phase. Endothelial cell release of FGF‐2 in high glucose leads to cell cycle progression, which makes cells more susceptible to TNFα‐induced cell death. These data suggest that growth factor outcomes in high glucose depend on secondary mediators such as cytokines and stimulation cell cycle timing. J. Cell. Physiol. 217: 86–92, 2008. © 2008 Wiley‐Liss, Inc.
Three‐dimensional sonography of the placental and uterine spiral vasculature: Influence of maternal age and parity
Authors: Zalud Ivica , Shaha Steven 
Published: May 2008

AbstractPurpose.To test the influence of maternal age and parity on placental volume and uterine spiral vasculature volume, their mean gray value, and 3‐dimensional power Doppler (3DPD) indices in normal pregnancy.Methods.Women with a gestational age of 14–25 weeks were included in the study. Exclusion criteria included posterior placenta, structural or chromosomal anomaly, pregnancy complications, and unknown pregnancy outcome. 3D sonograms of the placenta and uterine spiral vasculature were obtained. Virtual Organ Computer‐aided AnaLysis imaging software was used to calculate the volumes, mean gray value, and 3DPD indices.Results.The study included 199 women, the majority of whom were Filipino, Japanese, or part Hawaiian. The placental volume, uterine spiral vasculature volume, mean gray value, and 3DPD indices were similar in all women regardless of maternal age. However, uterine spiral vasculature volume was significantly larger in women younger than 25 years, compared with women 35 years of age and older. Parity influenced all placental 3DPD indices, whereas placental volume, uterine spiral vasculature volume, mean gray value, and 3DPD indices remained unaffected.Conclusion.Maternal age influenced uterine spiral vasculature volume, whereas parity influenced all placental 3DPD indices. © 2008 Wiley Periodicals, Inc. J Clin Ultrasound, 2008