bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entrepreneurship Learning by Experiential Learning in Motivating Student Courage to Start an Enterprise
Authors: Asy’ari Qaiyim , Dwiaryanti Risca , Kunaifi Aang 
Published: February 2024

Entrepreneurship is the key to the progress and prosperity of a nation. Apart from being a driver of economic activity and the distribution of wealth, entrepreneurship is also an indicator to measure the progress of a country. Developed countries on average have a composition of 15-20% entrepreneurs of the entire population. In fact, Indonesia still has around 4% of entrepreneurs. Therefore, a comprehensive strategy is needed to create new entrepreneurs, one of which is by intensifying entrepreneurship learning in schools and universities. This paper aims to formulate and describe effective entrepreneurship learning through experiential learning methods.Through qualitative research methods with an case study approach, the research is compiled into a paper presented at an international conference on Islamic economy and law. Data were collected through primary sources, from interviews with students before and after implementing the experiential learning formula for one semester at a private university. From these findings, concepts and methods for implementing experiential learning in entrepreneurship learning are compiled. The results of this study indicate that to move students to dare to enter the world of entrepreneurship, strong motivation and applicable training are needed. Motivation and training directly in the field turned out to be able to raise the courage of students in greater numbers to engage in entrepreneurship. With the results of this study, it is hoped that entrepreneurship learning in schools and universities in particular will become more systematic and effective.
Interaction between Pipe Rotation and Cuttings Transport in Extended-Reach Drilling: Mechanism, Model, and Applications
Authors: Zhao Jun , Huang Wenjun , Gao Deli 
Published: February 2024

Summary
High drag and torque on drillstring and difficulty in hole cleaning are two major challenges in extended-reach drilling, and these two challenges are usually coupled with each other. However, previous studies considered the drillstring mechanics and hole cleaning as two relatively independent issues and studied them separately, which cannot fully reveal the complex mechanisms of pipe sticking and obtain proper drilling parameters to ensure hole cleaning and reduce drag and torque. To solve this problem, in this paper we present the concept of pipe-cuttings interaction and elaborate on the two interaction mechanisms, which are the effect of pipe rotation on cuttings transport and the effect of cuttings distribution on pipe load. Second, we introduce the stirring diffusion factor and pipe-cuttings contact stress to quantify the pipe-cuttings interaction, and we obtain their mathematical expressions through nonlinear regression of numerical simulation results. Third, we establish the mechanical pipe-cuttings interaction model by modifying and combining the tubular mechanical model and the cuttings transport model. Finally, we validate the interaction model and apply it to a case study of extended-reach drilling in the South China Sea. The results show that the interaction model is of high prediction accuracy and is superior to the conventional tubular mechanical and cuttings transport models because the interaction between pipe rotation and cuttings transport is sufficiently considered. The problem of poor hole cleaning for a 12¼-in. section is serious, and pipe sticking occurs frequently for the extended-reach well in the case study because the stationary bed height is more than 10% in actual drilling. It is usually difficult to ensure hole cleaning of a 12¼-in. section by only optimizing a single parameter of rate of penetration (ROP), flow rate, or rotational speed, but optimizing two of the parameters at the same time can achieve better results. Lastly, we establish a recommended chart of drilling parameters, which can aid engineers in making comprehensive recommendations on drilling parameters.
Paracetamol (N-acetyl-para-aminophenol) disrupts early human embryogenesis
Authors: Kristensen David , Nielsen Brian , Petersen Morten , Martin-Gonzalez Javier , Holmberg Christian , Mjøseng Heidi , Frederiksen Hanne , Rosenthal Cristal , Jørgensen Emma , Serup Palle , Christensen Sarah , Petersen Kathrine , Kristiansen Karsten , Jørgensen Niklas , Hay-Schmidt Anders , Pedersen Per , Juul Anders , Pinborg Anja , Ziebe Søren , Lindenberg Svend , Elers Jimmi , Lindenberg Frederikke , Zedeler Anne , Christensen Søren 
Published: February 2024

Abstract
It is estimated that 10–40% of all human conceptions fail around implantation1-7. Genetics explain ≈ 50% of early embryonic loss, leaving a substantial part of early loss without a known cause8,9. Smoking and alcohol are known risk factors for spontaneous abortion, indicating the importance of the chemical environment during embryonic development10. Here we show that paracetamol (N-acetyl-para-aminophenol (APAP); otherwise known as acetaminophen), the recommended medication for pregnant people for treatment of mild to moderate pain and fever11 and an environmental pollutant12-15, disrupts both mouse and human pre-implantation development. We found that APAP inhibited cell cycle progression, likely through ribonucleotide reductase, resulted in blockage of DNA synthesis across all model systems, and reduced pregnancy outcomes in mouse models. At concentrations found in the reproductive system of women after standard administration, APAP exposure decreased human cleavage stage embryo cell numbers or caused direct embryonic fatality. Similar exposure to human blastocyst stage embryos for 6 h resulted in decreased DNA synthesis as well as morphological changes. Our data demonstrate that a widely used mild analgesic and environmental pollutant might result in embryonic loss and provide a foundation for understanding environmentally caused cell cycle inhibition in other processes during development.
Preliminary Field Validity of Free-Living Energy Expenditure Estimation in Wheelchair Users with Spinal Cord Injury via Wearable Device-based Models
Authors: Ding Dan , Huang Zijian , Veerubhotla Akhila , DeLany James 
Published: February 2024

Abstract
Study Design
Cross-sectional validation study.
Objectives
To develop a raw acceleration signal-based random forest (RF) model for predicting total energy expenditure (TEE) in manual wheelchair users (MWUs) and evaluate the preliminary field validity of this new model along with four existing models published in prior literature using the Doubly Labeled Water (DLW) method.
Setting
General community and research institution in Pittsburgh, USA.
Methods
A total of 78 participants’ data from two previous studies were used to develop the new RF model. A seven-day cross-sectional study was conducted to collect participants’ free-living physical activity and TEE data, resting metabolic rate, demographics, and anthropometrics. Ten MWUs with spinal cord injury (SCI) completed the study, with seven participants having valid data for evaluating the preliminary field validity of the five models.
Results
The RF model achieved a mean absolute error (MAE) of 0.59 ± 0.60 kcal/min and a mean absolute percentage error (MAPE) of 23.6%±24.3% on the validation set. For preliminary field validation, the five assessed models yielded MAE from 136 kcal/day to 1141 kcal/day, and MAPE from 6.1–50.2%. The model developed by Nightingale et al. in 2015 achieved the best performance (MAE: 136 ± 96 kcal/day, MAPE: 6.1%±4.7%), while the RF model achieved comparable performance (MAE: 167 ± 99 kcal/day, MAPE: 7.4%±5.1%).
Conclusions:
Two existing models and our newly developed RF model showed good preliminary field validity for assessing TEE in MWUs with SCI and the potential to detect lifestyle change in this population. Future large-scale field validation study and model iteration is recommended.
An evaluation of the implementation, adoption, and uptake of a model of remote e-consent within the PRE-DX study
Authors: Northgraves Matthew , Bradley Paul , Huang Chao , Sumpter Sarah , Miah Jamal , Cain Henry , Cohen Judith 
Published: February 2024

Abstract
Background:
Remote electronic consent (e-consent) is an area of growing interest but questions remain about how to successfully implement this in clinical research. There are potential benefits from adopting remote e-consent, but uncertainty exists regarding the barriers and facilitators to wider use. PRE-DX is a randomised controlled trial, comparing the effect of changing the time point when the Oncotype DX genomic assay is ordered on the patient management pathway in early-stage breast cancer. In this paper, we evaluated the implementation, adoption, and uptake of a model of remote e-consent and explored the impact of patient demographics, method of e-consent and site research staff training on successful implementation.
Methods:
Remote e-consent was the primary consent method in PRE-DX, with remote postal consent and face-to-face consent available as alternatives. At the mid-point of recruitment, virtual researcher meetings were held to facilitate site recruitment during which polls using Microsoft teams and open discussions about staff’s experience of the e-consent process were conducted. Demographic information collected as part of PRE-DX was used to describe the characteristics of participants for each consent method. Continuous variable of age for each consent method and overall are described according to mean, standard deviation, median, interquartile range, and minimum and maximum values. Categorical variables of consent methods, gender and ethnicity and poll results from the researcher meetings are reported as frequencies and percentages.
Results:
Three hundred and forty-one participants were recruited to PRE-DX. The predominant consent method was e-consent (n=213), followed by face-to-face consent (n=119) and postal (n=9). The comparison of patient demographics showed that the age of participants was similar across groups (e-consent: mean 57.7 ± 10.2 years, median 59.0 (13) years; postal: mean 55.8 ± 11.9 years, median 52 (17.5) years; face-to face: mean 58.5 ± 10.4 years, median 57 (14) years). No concerns regarding the e-consent method were expressed during the researcher meetings, but uptake varied across sites.
Conclusion:
Remote e-consent can be successfully implemented for recruitment of patients of different ages and is acceptable to site research teams. Alternative consent methods should be available to maximise recruitment.
Trial Registration:
PRE-DX (ISRCTN14337451), registered 16th August 2022.
SPP1+ macrophages in HR+ breast cancer are associated with tumour-infiltrating lymphocytes
Authors: Lee Hee Jin , Cha Su Min , Park Jung-Wook , Lee Yoon Jae , Lee Hee Jae , Lee Hyeonjin , Lee In Won , Gong Gyungyub , Park Sung Hee , Jeong Byung-Kwan 
Published: February 2024

Abstract
Breast cancer, categorised into hormone receptor-positive (HR+), HER2- positive (HER2+), and triple-negative (TNBC) subtypes, exhibits varied outcomes based on the number of tumour-infiltrating lymphocytes (TILs). Increased TIL levels are associated with better outcomes in HER2+ and TNBC, while HR+ individuals with high TIL levels show shorter survival but greater neoadjuvant chemotherapy response. To explore the divergent roles of TIL levels across various subtypes and their effect on immune cell composition, we employed single-cell RNA sequencing on 31 patients with breast cancer. HR+ breast cancer with high TIL levels (TIL-high) revealed increased SPP1+ macrophages, increased SPP1 expression in other monocytes/macrophages (mono/macro) subgroups, and enriched pathways associated with extracellular matrix (ECM) remodelling in mono/macro. Moreover, cell–cell interaction analyses revealed enhanced SPP1, MIF, and FN1 signalling in the interaction between SPP1+ macrophages and T-cells in TIL-high HR+ breast cancer. Spatial transcriptomics data highlighted the close proximity of SPP1+ macrophages, CD8+ T-cells, and CD4+ T-cells in TIL-high HR+ breast cancer. Our findings unveil the novel influence of SPP1+ macrophages on T-cells in TIL-high HR+ breast cancer, potentially explaining the poor prognosis and offering insights for targeted interventions.
Structure-function analysis of time-resolved immunological phases in metabolic dysfunction- associated fatty liver disease
Authors: Schmidt-Christensen Anja , Eriksson Gustaw , Laprade William M. , Pirzamanbein Behnaz , Hörnberg Maria , Linde Kajsa , Nilsson Julia , Skarsfeldt Mark , Leeming Diana J. , Mokso Rajmund , Verezhak Mariana , Dahl Anders , Dahl Vedrana , Önnerhag Kristina , Oghazi Massoud Rezaee , Mayans Sofia , Holmberg Dan 
Published: February 2024

Abstract

Metabolic dysfunction-associated steatohepatitis (MASH) is a common but frequently unrecognized complication of obesity and type 2 diabetes. The association between these conditions is multifaceted and involves complex interactions between metabolic, inflammatory, and genetic factors. Here we assess the underlying structural and molecular processes focusing on the immunological phase of MASH in the NIF mouse model and compare it to the human disease as well as other murine models. Histopathology together with synchrotron-radiation-based x-ray micro-computed tomography (SRµCT) was used to investigate structural changes within the hepatic sinusoids network in the NIF mouse in comparison to patients with different severities of MASH. A time-course, bulk RNA-sequencing analysis of liver tissue from NIF mice was performed to identify the dynamics of key processes contributing to the pathogenesis. Transcriptomics profiling of the NIF mouse revealed a gradual transition from an initially reactive inflammatory response to a regenerative, pro-fibrotic inflammatory response suggesting new avenues for treatment strategies that focus on immunological targets. Despite the lack of metabolic stress induced liver phenotype, a large similarity between the NIF mouse and the immunological phase of human MASH was detected. The translational value was further confirmed through comparative analyses with MASH patients and additional animal models. Finally, the impact of diets known to induce metabolic stress, was explored in the NIF mouse. An obesogenic diet was found to induce key physiological, metabolic, and histologic changes akin to those observed in human MASH.
Effectiveness of Minimally Invasive Sternal Compression Combined with the Nuss Procedure to Treat Pectus Carinatum Combined with Harrison Sulcus
Authors: Shang Zi Yin , Duan Xianlun , Hong Chun , Si Yuan 
Published: February 2024

Abstract
We aimed to investigate the feasibility of a minimally invasive sternal compression procedure c with Harrison sulcus treated from June 2015 to October 2022. All the patients underwent a minimally invasive sternal compression procedure combined with the Nuss procedure. The surgical conditions and postoperative outcomes of the patients and the satisfaction score of family members were analysed. Overall, the patients had a mean duration of surgery of 179.05 ± 36.01 min, intraoperative blood loss of 10.03 ± 2.77 mL, postoperative hospital stay of 6.89 ± 0.73 days, and postoperative satisfaction score of 89.4 ± 4.6. The incidence of surgical complications was 7.89%. The internal fixation stents were removed in 22 patients, and there was no recurrence during a follow-up 371.4 ± 6.3 days post-stent removal. These results were satisfactory. The use of a minimally invasive sternal compression procedure to treat this condition does not reduce the volume of the thorax after the procedure and results in an aesthetically pleasing incision, less complications, and fast postoperative recovery. Thus, it is a safe and effective method that is worthy of being promoted for clinical application.ombined with the Nuss procedure to treat pectus carinatum combined with Harrison sulcus. We retrospectively analysed the clinical data of 38 paediatric patients with pectus carinatum combined
Validating S100A16 as a prognostic and immunological biomarker in pan-cancer and lung adenocarcinoma through integrated Single-cell Sequencing Analysis
Authors: Shi Si , Yu Yao , Shang Yu , Wang Menghan , Xu Dandan , Chen Hong 
Published: February 2024

Abstract
Background
S100A16 belongs to the S100 protein family, exhibiting different expression levels across several human tumors. S100A16 upregulation in many malignancies suggests its potential role in malignant transformation. However, the specific involvement of S100A16 in lung adenocarcinoma (LUAD) remains unclear.
Methods
This study utilized RNA sequencing and protein expression data from the Cancer Genome Atlas (TCGA) and the Human Protein Atlas (THPA) databases to scrutinize the expression of S100A16 and its associations with patients’ prognosis, clinicopathological characteristics, tumor microenvironment (TME), immune cell infiltration, expression of immune checkpoint genes, and relevant signaling pathways in LUAD. Employing ESTIMATE and CIBERSORT algorithms alongside Gene Set Enrichment Analysis (GSEA), we investigated the underlying mechanisms by which LUAD is involved in the TME. Additionally, we used single-cell sequencing to measure the role of S100A16 at the cellular level and dissect the effect on treatment response in LUAD.
Results
S100A16 was highly expressed in LUAD. As an independent prognostic marker, S100A16 expression was correlated with adverse outcomes. Its expression levels were positively correlated with the clinical TN stage and LUAD grade. GO and KEGG analyses revealed the predominance of molecules positively associated with S100A16 expression in LUAD, concentrating on pathways related to cellular signaling, motility, morphology, and cell interactions. The high S100A16 group showed a significantly higher TME score compared with the low S100A16 expression group. Immune cells, including M1 macrophages, memory B cells, activated NK cells, plasma cells, and naive B cells, were positively associated with S100A16 expression in LUAD. Furthermore, a positive correlation was observed between S100A16 and the expression of most immune checkpoint genes. Patients with high S100A16 expression demonstrated lower IC50 values for drugs such as 5-fluorouracil, bortezomib, cisplatin, cytarabine, docetaxel, doxorubicin, etoposide, and vinorelbine, suggesting that S100A16 overexpression increased sensitivity to these treatments in LUAD. This study provides novel insights into the role of S100A16 in LUAD and associated signaling pathways.
Conclusions
S100A16 is an independent prognostic marker in LUAD, intricately linked to the TME, immune cell infiltration, immune checkpoint expression, and response to treatment. This study underscores the potential of S100A16 as a prognostic marker in LUAD, advancing cancer treatment.
Assessment of the Association of Repeated Vaginal Examination with Early Onset Neonatal Sepsis Hospital Based Follow Up Study
Authors: Lemma Ketsela , Berhane Yemane 
Published: February 2024

Abstract

Background: Sepsis is the 3rd leading cause of neonatal mortality in Ethiopia contributing for 16 % of neonatal death. Ina hospital study neonatal sepsis was the leading diagnosis at admission and second leading cause of neonatal death at the neonatal intensive care unit. Among other factors repeated vaginal examination during labor is known to contribute for sepsis in low-income settings. However, there is limited evidence in the Ethiopian setting.
Objective: The objective of this study was to examine the association between number of vaginal examination and early onset neonatal sepsis.
Methods: The study was conducted at Gandhi memorial Hospital, a public maternity and newborn care hospital. We followed 672 mother newborn pairs by phone until 7 days of age to detect clinical sepsis. Data were analyzed using SPSS version 20 software. Adjusted odds ratio (AOR) with corresponding 95% confidence interval (CI) was used to show strength of associations and variables with p value <0.05 were considered to be statistically significant.
Results: The incidence of early onset neonatal sepsis was found to be 20.83% (95% CI 17.60, 24.00). Having a frequent vaginal examination (four or more times) during labor and delivery was strongly associated with development of early onset neonatal sepsis, (ARR 2. 69 ;95 CI: 1.08, 6.70 ) after controlling for some common other risk factors including premature rupture of membrane and induced labor.
Conclusion: Frequent digital vaginal examination significantly increases the risk of early onset neonatal sepsis. Health professionals caring for laboring mother must limit the number of vaginal examinations to reduce the risk of neonatal sepsis which has a huge negative implication on the survival of the newborns. We also recommend further study using either cord blood or neonatal blood culture to better diagnose early onset neonatal sepsis objectively.
Clinical features and prognostic factors of IV combined small cell lung cancer: A Propensity Score Matching Analysis
Authors: cai shanshan , Yang Weichang , Li Zhouhua , Huang Xiaotian , Li Jinbo , Luo Hongdan , Ye Xiaoqun 
Published: February 2024

Abstract

Background
Nowadays, the characteristics and treatment of combined small-cell lung carcinoma (CSCLC) remain controversial. This study aimed to analyze the feature of clinical characteristics, survival outcomes and treatment modalities among IV CSCLC, IV SCLC and IV NSCLC, to provide more evidence for the study of IV CSCLC
Methods
All CSCLC, SCLC and NSCLC patient data were obtained from the SEER database (2010–2020). Pearson's χ2 test was used to compare the differences in clinical characteristics. Propensity score matching (PSM) was utilized to balance the bias of the variables between patients. Univariate and multivariate Cox proportional hazards regression analyses were performed to identify prognostic factors. KM analysis was used to calculate survival.
Results
A total of 493 patients with IV CSCLC, 35503 patients with SCLC, 122807 patients with IV NSCLC were included in this study. The demographic characteristics and tumor characteristics of three groups were different. Before PSM, there were significant difference in OS and CSS among IV CSCLC, IV SCLC and IV NSCLC, After PSM, there was significant difference in OS and CSS between the IV CSCLC and IV NSCLC. Risk/protective factors for OS and CSS were different in three groups. Chemotherapy, radiotherapy, surgery all can improve survival time of IV CSCLC. Chemotherapy combine surgery can significantly improve OS and CSS in patients with IV CSCLC (10.0 months and 16.0 months), chemotherapy alone was also a good choice for some IV CSCLC patients who have already lost the opportunity for surgery at the time of first diagnosis.
Conclusions
These results indicated that the prognosis and clinical characteristics IV CSCLC, IV SCLC and IV NSCLC were significant difference. Surgery combined chemotherapy was the best treatment in patients with IV CSCLC and chemotherapy alone was a good choice for patients who have lost the indication of surgery.