bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global signatures of the human mesenchymal stromal cell secretome: A comparative proteomic analysis of iPSC and tissue-derived MSC secretomes before and after inflammatory licensing
Authors: Hodgson-Garms Margeaux , Moore Matthew , Martino Mikaël , Kelly Kilian , Frith Jessica 
Published: April 2024

Abstract

Multipotent mesenchymal stromal cells (MSCs) are one of the most heavily studied cell therapies worldwide, with much of their therapeutic potential underpinned by their complex secretory profiles. Like MSCs themselves, their secretome varies significantly between donors, sources, and according to microenvironmental cues. As such, there remains a lack of consensus as to the global nature of the MSC secretome, its source-based heterogeneity, and the dynamic changes it undergoes in response to inflammatory licensing. A full understanding of these differences is essential in understanding the mechanisms that drive MSC-based tissue repair and in optimising the properties of MSCs for cell therapies. This study used liquid chromatography tandem mass spectrometry (LC/MS-MS) to characterise and compare the secretomes of 13 MSC lines, sourced from bone marrow (BM.MSCs), umbilical cord (UC.MSCs), and adipose tissue (AT.MSCs), alongside multiple batches of clinical and commercial grade induced pluripotent stem cell derived MSCs (iMSCs), all under both resting and inflammatory licensed conditions. We confirm, for the first time, that iMSCs successfully recapitulate the process of inflammatory licensing, validating their comparability to tissue-derived MSCs and providing important support for their application as an immunotherapy. We identify a global and dichotomous signature of the MSC secretome and inflammatory licensing, where resting secretomes are defined by prominent extracellular matrix (ECM) proteins and overrepresentation of pro-regenerative and wound healing processes, while licensed secretomes downregulate these factors in favour of chemotactic and immunosuppressive proteins and immunomodulatory processes. Furthermore, under both resting and licensed conditions, MSC secretomes separate based on source, with iMSC and UC.MSC secretomes more similar to each other, containing higher concentrations of proteins indicating proliferative potential and telomere maintenance, while adult tissue-derived, BM.MSC and AT.MSC secretomes contained more fibrotic and ECM proteins This dataset provides a detailed atlas of resting and licensed MSC secretomes and generates insights into the molecular mechanisms underlying the differences observed between MSC secretomes from different sources or functional states. Going forward, this will inform the design of more effective MSC-based therapies by identifying the most suitable MSC source for a particular application, and allow the development of tailored culture conditions and/or preconditioning methods to enhance the therapeutic potential of these cells, potentially identifying specific factors that can be adapted for pharmaceutical intervention.
Alterations in gut microbiota composition, plasma lipids, and brain activity, suggest inter-connected pathways influencing malnutrition-associated cognitive and neurodevelopmental changes.
Authors: O'Sullivan Justin , Portlock Theo , Sharma Talat , Kakon Shahria , Hartjen Berit , Pook Chris , Wilson Brooke , Bhuttor Ayisha , Ho Daniel , Don Inoli , Engelstad Anne-Michelle , Lorenzo Renata Di , Greaves Garrett , Rahman Navin , Kelsey Caroline , Gluckman Peter , Forrester Terrence , Nelson Charles 
Published: April 2024

Abstract

Malnutrition affects over 30 million children annually and has profound immediate and enduring repercussions, with nearly half of child deaths under five linked to malnutrition. Survivors face lasting consequences, including impaired neurocognitive development, leading to cognitive and behavioural deficits, impacting academic performance and socioeconomic outcomes. Despite extensive literature on malnutrition’s associations with infections, metabolism, microbiome, and genomics, knowledge gaps persist around how these changes are mechanistically connected. The objective of this study was to identify gut microbiome mediated associations between Moderate Acute Malnutrition (MAM) and cognitive development. Despite limitations, this study provides evidence supporting the hypothesis that inter-connected pathways between the gut microbiome, microbial metabolism mediate plasma lipids, brain activity, and cognitive functions. Causality between gut microbiome, plasma metabolite changes, and MAM phenotype remains unclear, necessitating further research. However, these findings offer insights to optimize interventions for MAM-associated neurodevelopmental changes.
The Change in Body Temperature, Not Acute-Phase Reaction, Predicts anit-Osteoporosis Efficacy after the First Administration of Zoledronic Acid in a self-control study
Authors: Du Yanping , Yu Weijia , Gou Haixin , Lei Yiming , Zhang Tongkai , Tang Wenjing , Chen Minmin , Li Huilin , Cheng Qun 
Published: April 2024

Abstract

Background:
Acute-phase reactions (APRs) are common among people who are treated for the first time with zoledronate (ZOL). The current view is that both the APRs caused by ZOL and its efficacy are related to the mevalonic acid pathway. However, the relationship between APR and ZOL efficacy remains unclear.
Methods：
This study was a self pre - post control study involving postmenopausal women with osteoporosis in Shanghai, China, for 1 year. A total of 108 patients withan average age of 67.4±5.8 years were treated with 5 mg intravenous ZOL for the first time. Data on demographic characteristics, APRs , blood counts, bone turnover markers, including CTX and PINP, and bone mineral density (BMD) were collected.
Results：
1) The results did not show a relationship between the APRs and changes in bone turnover markers and BMD but showed that changes in body temperature (T) within 3 days after administration were positively correlated with changes in BMD of LS at Month 6 and Month 12 (r=0.304 P<0.05, r=0.387 P<0.01). 2) This effect was mainly mediated by changes in serum CTX (b=0.046, 95% CI [0.0010-0.0091]). 3) TheROC curve showed that when T increased by 1.95°C, the sensitivity and specificity of judging the clinically important change in LS BMD after 1 year were the best.
Conclusions:
In this study, we tested the hypothesis that people with elevated body T after initial ZOL treatment had greater BMD improvements and better outcomes.
Characterization of Language Abilities and Semantic Networks in Very Preterm Children at School-age
Authors: Décaillet Marion , Christensen Alexander P. , Besuchet Laureline , Huguenin-Virchaux Cléo , Fumeaux Céline J. Fischer , Denervaud Solange , Schneider Juliane 
Published: April 2024

Abstract

It has been widely assessed that very preterm children (< 32 weeks gestational age) present language and memory impairments compared to full-term children. However, differences in their underlying semantic memory structure have not been studied yet. Nevertheless, the way concepts are learned and organized across development relates to children’s capacities in retrieving and using information later. Therefore, the semantic memory organization could underlie several cognitive deficits existing in very preterm children. Computational mathematical models offer the possibility to characterize semantic networks through three coefficients; average shortest path length (i.e., distance between concepts), clustering (i.e., local interconnectivity), and modularity (i.e., vocabulary enrichment). Here we assessed these coefficients in 38 very preterm schoolchildren (aged 8–10 years) compared to 38 full-term schoolchildren (aged 7–10 years) based on a verbal fluency task. Using semantic network analysis, very preterm children showed a lower interconnectivity at a local level than full-term children. However, we found no differences between very preterm and full-term children regarding their average shortest path length between concepts and their modularity at a global level. These findings provide preliminary evidence that very preterm children demonstrate subtle impairments in the organization of their semantic network, encouraging the adaptation of the support and education they receive.
Unraveling the Gut Microbiome's Role in Kidney Stone Formation: Insights from Fecal Microbiota Transplantation Studies
Authors: Hunthai Sittiphong , Usawachintachit Manint , Taweevisit Mana , Srisa- Monpichar , Anekamol Weerapat , Tosukhowong Piyaratana , Rattanachaisit Pakkapon , Chuaypen Natthaya , Dissayabutra Thasinas 
Published: April 2024

Abstract

Emerging research on the microbiome highlights the significant role of gut health in the development of kidney stones, indicating that an imbalance in gut bacteria, or dysbiosis, can influence the formation of stones by altering oxalate metabolism and urinary metabolite profiles. Notably, the presence of specific bacteria such as Enterococcus and Oxalobacter spp., known to affect oxalate absorption, is linked to urolithiasis, prompting further investigation into the gut's role in calcium oxalate stone disease. This study investigates the effects of gut dysbiosis on urolithiasis through fecal microbiota transplantation (FMT) from patients to rats and its impact on urinary mineral excretion and stone formation. Fecal samples from eight patients with calcium oxalate stones and ten healthy volunteers were collected to assess the gut microbiome. These samples were then transplanted into Wistar rats for a duration of four weeks. Post-transplantation, we evaluated changes in the fecal gut microbiome profile, urinary mineral excretion rates, and the expression levels of intestinal zonula occluden-1 (ZO-1), SLC26A6, and renal NF-κB. Patients with urolithiasis exhibited increased levels of urinary calcium and oxalate, alongside decreased citrate excretion and a heightened urinary supersaturation index, with a notable abundance of Bacteroidota. Rats receiving urolithiasis-FMT showed similar disturbances, including elevated urinary oxalate, increased pH, and supersaturation index, despite negative renal pathology. However, significant elevation of the expression of renal NF-κB, intestinal SLC26A6, and a reduction in ZO-1 expression were observed. Additionally, an increased abundance of Bacteroidota, particularly Muribaculaceae, was detected in the feces of urolithiasis rats. Conclusively, the consistent increase in Bacteroidota abundance across both urolithiasis patients and FMT-treated rats is linked to altered intestinal barrier function, hyperoxaluria, and renal inflammation. These findings suggest that gut dysbiosis, characterized by an overgrowth of Bacteroidota, plays a crucial role in the pathogenesis of calcium oxalate urolithiasis, underscoring the potential of targeting gut microbiota as a therapeutic strategy.
Unlocking Travel Desires: Exploring Configurations of Gratifications and Epidemic Concerns in Short Video Context
Authors: Zhang Shuhua , Wu Yanni , Chen Hongquan , Zhu Yurong , Chai Huihua 
Published: April 2024

Abstract

To promote tourism recovery and growth, it's crucial to comprehend the factors influencing travel intention amid epidemic threats like COVID-19. Grounded in the uses and gratifications (U&G) theory and employing fuzzy-set qualitative comparative analysis (fsQCA), this study aims to elucidate the causal patterns of hedonic, social, and utilitarian gratifications intersecting with epidemic concerns, shaping high travel intention in short video context. 298 college students' data unveil six configurations elucidating high travel intention. This research offers fresh perspectives on how U&Gs and epidemic concerns interact, aiding in devising strategies to boost tourism industry prosperity.
Simultaneous or sequential kidney-liver transplantation in primary hyperoxaluria
Authors: Arena Maria , Labbadia Raffaella , Cappoli Andrea , Spagnoletti Gionata , Camassei Francesca Diomedi , Emma Francesco , Spada Marco , Guzzo Isabella 
Published: April 2024

Abstract

Primary hyperoxaluria type 1 is responsible for pediatric kidney failure in 1 to 2% of cases. Novel therapies based on RNA interference are changing the natural history of the disease. However, for those who will progress to kidney failure, and for patients living in countries that cannot afford these expensive therapies, liver-kidney transplantation may remain the only efficient therapy. The aim of the study was to evaluate the outcome of patients with primary hyperoxaluria type 1 who received simultaneous or sequential liver-kidney transplantation. We retrospectively evaluated 10 patients, five patients received a simultaneous transplantation, and five underwent sequential transplantation with a median postponement of the kidney transplantation of 8 months (range 4–20). Median follow up was 3.2 years (range 1.6–11). Median estimated glomerular filtration rate at 6 and 12 months was 81.2 (range: 45.7-108.8) and 79.3 ml/min/1.73m2(range 54.7-112.1) in patients who underwent simultaneous transplantation, and 45.7 (range 34.5–86.7) and 38.3 ml/min/1.73m2(range 29.9–77.5) in those with sequential transplantation (p:NS). Biopsies performed at 6 and 12 months showed precipitation of calcium oxalate crystals in all patients except one, demonstrating the recurrence of deposition despite the delay between liver and kidney transplantation. No differences in kidney function or in post-transplant renal oxalate precipitations were observed between patients that underwent bilateral nephrectomy and those who did not. None of the patients has lost the kidney graft at the last follow-up. Our study shows that adapting the transplant strategy to individual cases, patients with primary hyperoxaluria type 1 can be successfully treated.
Accessing patient satisfaction for palliative care outpatient telemedicine services at a tertiary care hospital in Karachi, Pakistan.
Authors: Jabeen Ismat , Qureshi Asra , Assad Sidra , Qamar Uqba , Waqar Muhammad Atif 
Published: April 2024

Abstract
Background:
Telemedicine has been projected as one way to improve access to palliative care services for patients with serious illness, delivering health care services remotely given a shortage of trained physicians and available specialty services. This technology is being used extensively to improve quality of life of patients needing palliation during unprecedented times of COVID-19 pandemic. However, its efficacy has not been evaluated, specifically in palliative care specialty, where it is instrumental for healthcare access. We aimed to evaluate satisfaction and factors related to satisfaction with telemedicine appointment system and palliative care team during virtual outpatient palliative care telemedicine consultations.
Methods
This cross-sectional study was conducted on patients seen in adult palliative care telemedicine clinics between February 2020 to March 2022 at a tertiary care hospital in Pakistan.
Results
A total of 130 participants participated, with a mean age of 63.2 years, n = 61 (47%) males and n = 69 (53%) females. There were n = 76 (58.4%) initial visits and n = 54 (41.5%) follow-ups. 94% of the participants agreed with healthcare access, 92% with the quality of care, 92% with patient-physician interaction, 77% with convenience, 91% wanted to continue teleconsultation and 88% found it cost-effective. Besides this, 46% of participants expressed that they were worried about privacy breach.
Conclusion
Telemedicine is an innovative approach. Participants find it accessible, cost-effective, convenient and of good quality. In future, it can be implemented and should be promoted in the post pandemic landscape as an effective patient care modality to address enormous unmet needs of patients with functional frailty or living in remote area.
Does the odour of human stress or relaxation affect dogs’ cognitive bias?
Authors: Parr-Cortes Zoe , Müller Carsten Theodor , Talas Laszlo , Mendl Michael , Guest Claire , Rooney Nicola Jane 
Published: April 2024

Abstract

Dogs can discriminate between stressed and non-stressed odour samples from humans, but the effect on their cognition and emotional state is previously unstudied. Eighteen dogs each participated in three cognitive bias sessions: baseline (no odour), stress odour and relax odour, with the order of the two test odours counterbalanced across dogs. The odours were combined breath and sweat samples from three volunteers showing measurable stress responses during a stress test compared to a relaxing activity. We found that dogs were significantly less likely to approach an ambiguous (near negative) location in the presence of stress odour. They were also significantly less likely to approach the trained unrewarded (negative) location and more likely to approach the rewarded (positive) location in the presence of both human odours. Learning over sessions and the order in which odours were presented also had significant effects, with the combination of stress odour and the last testing session having the greatest effect. This is the first study to show that in the absence of visual or auditory cues, olfactory cues of stress from an unfamiliar person may affect dogs’ cognition and learning. This could have important consequences for dog welfare and working performance.
Research on CO Dispersion and Isolation Door Anti-virus Facilities in Refuge Chamber: Experiment and Simulation
Authors: Li Zongxiang , Huang Shuoran , Li Lin , Si Junhong , Wu Ji 
Published: April 2024

Abstract

In order to study the process of underground harmful gas CO dispersion into the refuge chamber and improve the survival rate of trapped personnel, a simple model of underground refuge chamber and main roadway was built. The influence of temperature and pressure air volume on CO dispersion in refuge chamber has been studied by analog experiment and numerical simulation, and the reliability of simulation results is verified. A flexible isolation door for controlling harmful gases in refuge chamber was designed, and the influence of the installation of the isolation door on CO dispersion has been compared and analyzed. The results show that when the temperature of the refuge chamber is lower than that of the harmful gas, CO will invade the refuge chamber from the upper part of the protective sealed door, and vice versa. The change stage of CO concentration in transition chamber can be divided into sudden drop stage, slow decline stage and unstable stage; The change stage of CO concentration in survival chamber can be divided into rapid decline stage and stable stage. When the temperature of the main roadway is 50°C and the temperature of the refuge chamber is 20°C, the pressure air volume within the safe range (24 ppm) of the CO concentration is reduced to 69.6% of that without the isolation door, effectively prevent harmful gases in the main alley from invading the refuge chamber.
YOLOv8-BCC: Lightweight Object Detection Model Boosts Urban Traffic Safety
Authors: Jun Tang , Lai Zhouxian , Ye Caixian , Xu lijun 
Published: April 2024

Abstract

With the rapid development of urbanization, the role of urban transportation systems has become increasingly prominent. However, traditional methods of traffic management are struggling to cope with the growing demands of traffic and the complexity of urban environments. In response to this situation, we propose the YOLOv8-BCC algorithm to address existing shortcomings. Leveraging advanced technologies such as CFNet, CBAM attention modules, and BIFPN structure, our algorithm aims to enhance the accuracy, real-time performance, and adaptability of urban traffic intelligent detection systems. Experimental results demonstrate significant improvements in detection accuracy and real-time performance compared to traditional methods. The introduction of the YOLOv8-BCC algorithm provides a robust solution for enhancing urban traffic safety and intelligent management.
Variations in deep-sea methane seepage linked to millennial-scale changes in bottom water temperatures ~50–6 ka, NW Svalbard margin
Authors: Rasmussen Tine L. , Altuna Naima El bani , Thomsen Erik 
Published: April 2024

Abstract

During the last glaciation, the northern hemisphere experienced profound millennial-scale changes (termed Dansgaard-Oeschger (DO) events) in atmospheric and oceanic temperatures. In the North Atlantic, the fluctuations resulted in extremely unstable bottom water conditions with bottom water temperatures (BWT) varying up to > 5°C. We have studied these environmental changes in a core from 1300 m water depth at Vestnesa Ridge, northwestern Svalbard margin to investigate a possible connection between BWT and seepage of methane from the seafloor covering the period ~ 50–6 ka. Beneath Vestnesa Ridge, gas hydrates containing vast amounts of methane are kept stable due to the high pressure and low temperatures. Release of gas is shown by numerous pockmarks on the seafloor. The pockmarks at 1300 m water depth are presently inactive, but they bear witness of earlier activity. Our study shows that from ~ 50–6 ka, the core site experienced repeated increases in BWT and in the emissions of gas, both following the pattern of the DO-events. This correspondence in time scale indicates that BWT was the primary forcing factor for the variability in methane release. However, the releases were delayed with up to > 1000 years compared to initial increase in BWT.
Skin-friendly, high pressure elasticity, high sensitivity PV/rGO/VF composite flexible sponge for intelligent physiological monitoring
Authors: Mao Yinfeng , Hong Dingsheng , Zhou Xinzheng , Chang Taotao , Shen Lingna , Wei Kaitai , Cui Yifan , Li Ping , Zhang Huanxia 
Published: April 2024

Abstract

3D graphene-based flexible nanosponges have shown great application potential in areas such as human motion tracking, health monitoring, and electronic skin. However, previously reported graphene-based flexible sensors typically focus on simple pressure sensing, lacking the capability to simultaneously detect both pressure and physiological signals. This limitation restricts their application in the field of intelligent wearable technology. In this study, a pressure-flexible composite sponge sensor was prepared using polyvinylidene fluoride (PVDF), viscose nonwoven fabric, and graphene oxide (GO) in a simple and cost-effective manner. Results showed that high-pressure electrospun PVDF inhibited the α-crystalline phase while promoting the transformation of diffraction peaks into the β-crystalline phase. Furthermore, high-temperature oxidation–reduction improved the crystallinity of β-phase crystals, enhancing the conductivity of PVDF/reduced GO/viscose fiber/polydimethylsiloxane ( PV/rGO/VF/P )sponges. With the increase of GO mass ratio, the thermal stability of the flexible composite device improved while weight loss decreased and resistance sensitivity increased. When compressed to 40%, the piezoelectric effect is most sensitive, and the composite sponge can fully recover under 60% compression. The addition of viscose fiber with a high swelling effect enables the composite sponge to precisely and sensitively detect the amount of sweat or glucose. This three-dimensional nanosponge can be applied in the design of ergonomic, physiologically monitoring smart wearable devices.