bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors for umbilical hernia in Holstein heifers during the first two months after birth
Authors: Steenholdt Christian , Hernandez Jorge 
Published: January 2006

AbstractObjective—To determine risk factors associated with identification of an umbilical hernia during the first 2 months after birth in Holstein heifers.Design—Case-control study.Animals—322 Holstein heifers born in a single herd (45 with an umbilical hernia and 277 without).Procedure—Risk factors that were examined included sire, whether the dam had a history of umbilical hernia, milk yield, duration of gestation, whether the dam had a history of dystocia, whether the heifer had a twin, birth weight, total serum protein concentration, and whether the heifer had an umbilical infection. Logistic regression was used to analyze risk factors.Results—Heifers born to sires with ≥ 3 progeny with an umbilical hernia were 2.31 times as likely to develop an umbilical hernia as were heifers born to sires with ≤ 2 progeny with an umbilical hernia. Heifers with umbilical infection were 5.65 times as likely to develop an umbilical hernia as were heifers without umbilical infection.Conclusions and Clinical Relevance—Sire and umbilical infection were associated with risk of an umbilical hernia during the first 2 months of life in Holstein heifers. Attributable proportion analysis indicated that the frequency of umbilical hernias in Holstein heifers with umbilical infection would have been reduced by 82% if umbilical infection had been prevented. (J Am Vet Med Assoc2004;224:1487–1490)
Evaluation of outcome of otitis media after lavage of the tympanic bulla and long-term antimicrobial drug treatment in dogs: 44 cases (1998–2002)
Authors: Palmeiro Brian S. , Morris Daniel O. , Wiemelt Staci P. , Shofer Frances S. 
Published: January 2006

AbstractObjective—To evaluate the outcome of otitis media in dogs after video-otoscopic lavage of the tympanic bulla and long-term antimicrobial drug treatment.Design—Retrospective study.Animals—44 dogs with otitis media treated in an academic referral practice.Procedure—Medical records were reviewed for signalment, duration of ear canal disease, previous medical treatments, dermatologic diagnosis, results of cytologic examination and microbial culture of ear canal exudate, findings during video-otoscopy, medical treatment, days to resolution, and maintenance treatments prescribed. Four independent variables (age, duration of ear canal disease prior to referral, use of corticosteroids in treatment regimens, and infection withPseudomonas aeruginosa) were evaluated statistically for potential influence on time to resolution.Results—Mean ± SD (range) duration of ear canal disease prior to referral was 24.9 ± 21.6 (3 to 84) months. Otitis media in 36 dogs resolved after lavage of the tympanic bulla and medical management; mean ± SD (range) time to resolution was 117 ± 86.7 (30 to 360) days. Time to resolution was not significantly influenced by any variable evaluated. Three dogs were lost to follow-up, and 4 dogs eventually required surgical intervention. Seven of 36 dogs in which otitis had resolved relapsed; 4 required additional lavage procedures.Conclusions and Clinical Relevance—Results indicate that lavage of the tympanic bulla combined with medical management is an effective and viable option for treatment of otitis media in dogs. (J Am Vet Med Assoc2004;225:548–553)
Use of the meridian test for the detection of equine herpesvirus type 1 infection in horses with decreased performance
Authors: Chvala Sonja , Nowotny Norbert , Kotzab Erich , Cain Marvin , Hoven René van den 
Published: January 2006

Abstract

Objective—To evaluate use of the acupuncture
meridian test for detection of recent or recently reactivated
equine herpesvirus type 1 (EHV-1) infection in
horses with decreased performance.

Design—Case-control study.

Animals—40 horses.

Procedure—Physical and neurologic examinations
were performed, and acupuncture points on the bladder
meridian were tested for sensitivity reactions in
case and control horses. Polymerase chain reaction
assays were performed to determine whether EHV-1
or equine herpesvirus type 4 (EHV-4) DNA could be
detected in peripheral blood mononuclear cells.
Complement fixation (CF) tests for detection of antibodies
against EHV-1 and EHV-4 and virus neutralization
(VN) tests for detection of antibodies against
EHV-1 were performed on paired serum samples
obtained 3 weeks apart.

Results—There was a significant difference in skin
sensitivity in the cervical, sacral, and gluteal regions
and flank between case and control horses. By use
of the meridian test, all case horses were sensitive
to manipulation of all acupuncture points believed to
be associated with EHV infections, whereas only a
few control horses were sensitive at an occasional
point. Equine herpesvirus type 1 or EHV-4 viremia
was not detected in any horses. Mean ± SD VN antibody
titers against EHV-1 were not significantly different
between the 2 groups. Mean ± SD CF antibody
titers against EHV-1 obtained 3 weeks after the
initial samples were higher in case horses than control
horses; however, unequivocal seroconversion
was not detected.

Conclusions and Clinical Relevance—Results of the
meridian test in case horses were associated with sensitivity
reactions similar to those detected by physical
and neurologic examinations; however, an unequivocal
association with EHV-1 or EHV-4 infection was not
detected. ( J Am Vet Med Assoc 2004;225:554–559)
Isolation of Salmonella spp from the environment of dairies without any history of clinical salmonellosis
Authors: Peek Simon F. , Hartmann Faye A. , Thomas Chet B. , Nordlund Kenneth V. 
Published: January 2006

AbstractObjective—To determine whether Salmonella spp could be isolated from the environment of free stall dairies in Wisconsin without any history of clinical salmonellosis and determine the serotype and antimicrobial susceptibility of anySalmonellaisolates recovered from the environment.Design—Cross-sectional study.Study Population—20 free stall dairies with no history of clinical salmonellosis.Procedures—Dairy owners completed a questionnaire regarding management and production practices. Multiple swab samples were obtained from throughout the free stall facilities and submitted for bacterial culture forSalmonellaspp. Odds ratios were calculated to compare herd-level risk factors between dairies from whichSalmonellaorganisms were isolated and herds from whichSalmonellaorganisms were not isolated.Results—Salmonellaorganisms were isolated from 9 of the 20 (45%) dairies.Salmonellaserotype Meleagridis was isolated from 4 dairies,SMeleagridis andSKentucky were isolated from 2 dairies,SMeleagridis andSCyprus were isolated from 1 dairy,SCerro was isolated from 1 dairy, andSCorvallis was isolated from 1 dairy. All isolates were susceptible to all antimicrobial agents tested. None of the potential risk factors analyzed demonstrated a significant association with an increased likelihood of isolatingSalmonellaspp.Conclusions and Clinical Relevance—EnvironmentalSalmonellacontamination was demonstrated on free stall dairies with no history of clinical salmonellosis. (J Am Vet Med Assoc2004;225:574–577)
Deployment morbidity among search-and-rescue dogs used after the September 11, 2001, terrorist attacks
Authors: Slensky Kimberly A. , Drobatz Kenneth J. , Downend Amanda B. , Otto Cynthia M. 
Published: January 2006

AbstractObjectives—To determine characteristics, variables associated with deployment morbidity, and injuries and illnesses of search-and-rescue dogs associated with the Sept 11, 2001, terrorist attacks.Design—Historical cohort study.Animals—96 dogs.Procedure—Data collected included previous medical or surgical history, physical attributes of dogs, type and number of years of training, site of deployment, shift and hours worked, and number of days deployed. Combined morbidity was defined as 1 or more abnormalities of body systems, including traumatic injuries.Results—Handlers of 96 of the 212 dogs responded to the surveys. Fifty-nine dogs were deployed by the Federal Emergency Management Agency, 10 by police forces, and 27 as members of other search-and-rescue teams. Sixty-five dogs (incidence rate, 17 events/1,000 dog search hours) had combined morbidity during deployment. System-specific morbidity rates included gastrointestinal tract signs (5 events/1,000 dog search hours), cuts and abrasions mostly on the feet (5 events/1,000 dog search hours), fatigue (6 events/1,000 dog search hours), change in appetite (6 events/1,000 dogs search hours), dehydration (5 events/1,000 dog search hours), respiratory tract problems (2 events/1,000 dog search hours), heat exhaustion (2 events/1,000 dog search hours), and orthopedic or back problems (2 events/1,000 dog search hours). Dogs deployed to the World Trade Center were 6.6 times more likely to have combined morbidity, compared with dogs at the Pentagon.Conclusions and Clinical Relevance—Injury and illnesses occurred in most dogs and affected several organ systems, but all were minor. (J Am Vet Med Assoc2004;225:868–873)
Prevalence of anthelmintic resistant cyathostomes on horse farms
Authors: Kaplan Ray M. , Klei Thomas R. , Lyons Eugene T. , Lester Guy , Courtney Charles H. , French Dennis D. , Tolliver Sharon C. , Vidyashankar Anand N. , Zhao Ying 
Published: January 2006

AbstractObjective—To determine prevalence of anthelmintic resistance in cyathostome nematodes of horses in the southern United States.Design—Cross-sectional study.Animals—786 horses on 44 farms and stables in Georgia, South Carolina, Florida, Kentucky, and Louisiana.Procedure—Fecal egg count (FEC) reduction tests were performed on 44 large farms and stables. Horses on each farm were treated with an oral paste formulation of fenbendazole, oxibendazole, pyrantel pamoate, or ivermectin at recommended label dosages. A mixed linear model was fitted to the percentage reduction in FEC, accounting for differences among farms, states, ages, treatments, and treatment by state interactions.Results—By use of a conservative measure of resistance (< 80% reduction), the percentage of farms with anthelmintic-resistant cyathostomes was 97.7%, 0%, 53.5%, and 40.5% for fenbendazole, ivermectin, oxibendazole, and pyrantel pamoate, respectively. Mean percentage reductions in FEC for all farms were 24.8%, 99.9%, 73.8%, and 78.6% for fenbendazole, ivermectin, oxibendazole, and pyrantel pamoate, respectively. Pairwise contrasts between states for each treatment revealed that in almost all instances, there were no significant differences in results between states.Conclusions and Clinical Relevance—The prevalence of resistance found in this study was higher than that reported previously, suggesting that anthelmintic resistance in equine cyathostomes is becoming a major problem. Furthermore, data from these 5 southern states, which are geographically and physiographically distinct, were remarkably similar. This suggests that drug resistance in cyathostomes is highly prevalent throughout the entire southern United States and probably nationwide. (J Am Vet Med Assoc2004;225:903–910)
Evaluation of rumen transfaunation after surgical correction of left-sided displacement of the abomasum in cows
Authors: Rager Kimberly D. , George Lisle W. , House John K. , DePeters Edward J. 
Published: January 2006

AbstractObjective—To evaluate rumen transfaunation after surgical correction of left-sided displacement of the abomasum (LDA) in cows.Design—Prospective clinical trial.Animals—20 multiparous cows with LDA.Procedures—Cows with LDA were treated surgically (day 0). On days 0 (immediately after surgery) and 1, 10 cows each received 10 L of rumen fluid (transfaunated group) or 10 L of water (control group) via a stomach tube. Postoperative dietary dry-matter intake and milk yield of each cow were recorded daily for 5 days, beginning immediately after surgery. Blood and rumen fluid samples were collected prior to surgery and on days 1, 3, and 5 after surgery. Serum nonesterified fatty acid and β-hydroxybutyrate concentrations were measured. Volatile fatty acid and ammonia concentrations and pH of rumen fluid were determined. Urine specimens were collected and tested for ketones at 8 AM and 4 PM. Cows with ketonuria were treated with 50% dextrose solution administered IV at the time ketonuria was first detected. Cows with ketonuria were treated twice daily until ketonuria resolved.Results—All cows survived and completed their lactation. Daily and cumulative dry-matter intake and milk yield of cows in the transfaunated group were significantly greater than those of cows in the control group. Cows in the transfaunated group had significantly lower serum concentrations of β-hydroxybutyrate and significantly lower acetate-to-propionate ratios in rumen fluid on day 1 after surgery, compared with cows in the control group . Cows that received transfaunate required a significantly lower total volume of dextrose administered IV than control cows.Conclusions and Clinical Relevance—Benefits of rumen transfaunation of cows after surgical correction of LDA included a lesser degree of ketonuria, greater feed intake, and higher milk yield, compared with nontransfaunated cows. (J Am Vet Med Assoc2004;225:915–920)
Photo‐Polymerizable Autologous Growth‐Factor Loaded Silk‐Based Biomaterial‐Inks toward 3D Printing‐Based Regeneration of Meniscus Tears
Authors: Bandyopadhyay Ashutosh , Ghibhela Baishali , Shome Sayanti , Hoque Samsamul , Nandi Samit K. , Mandal Biman B. 
Published: February 2024

AbstractMeniscus tears in the avascular region undergoing partial or full meniscectomy lead to knee osteoarthritis and concurrent lifestyle hindrances in the young and aged alike. Here they reported ingenious photo‐polymerizable autologous growth factor loaded 3D printed scaffolds to potentially treat meniscal defects . A shear‐thinning photo‐crosslinkable silk fibroin methacrylate–gelatin methacrylate–polyethylene glycol dimethacrylate biomaterial‐ink is formulated and loaded with freeze‐dried growth factor rich plasma (GFRP) . The biomaterial‐ink exhibits optimal rheological properties and shape fidelity for 3D printing. Initial evaluation revealed that the 3D printed scaffolds mimic mechanical characteristics of meniscus, possess favourable porosity and swelling characteristics, and demonstrate sustained GFRP release. GFRP laden 3D scaffolds are screened with human neo‐natal stem cells in vitro and biomaterial‐ink comprising of 25 mg mL−1 of GFRP (GFRP25) is found to be amicable for meniscus tissue engineering. GFRP25 ink demonstrated rigorous rheological compliance, and printed constructs demonstrated long term degradability (>6 weeks), GFRP release (>5 weeks), and mechanical durability (3 weeks). GFRP25 scaffolds aided in proliferation of seeded human neo‐natal stem cellsand their meniscus‐specific fibrochondrogenic differentiation . GFRP25 constructs show amenable inflammatory response in vitro and in vivo. GFRP25 biomaterial‐ink and printed GFRP25 scaffolds could be potential patient‐specific treatment modalities for meniscal defects.
Study of oscillation characteristics for quartz crystal oscillators based on equivalent multi‐physics model
Authors: Chen Zhiyu , Zhu Yueyan 
Published: February 2024

AbstractIn recent years, high‐performance quartz‐crystal oscillators (XOs) for integrated circuits have been receiving considerable attention due to their featuring low voltage and high‐frequency stability. However, recent studies tend to focus solely on the impact of temperature as a single factor on crystal oscillator circuits, overlooking the circuit structure of the crystal oscillator itself. In this paper, a novel four‐parameter crystal model of XOs is detailed demonstrated, and analyzed to interpret typical XO oscillation characteristics at room temperature. The relationship between the RLC circuit and the oscillation was investigated. Meanwhile, the study delves into the various factors that influence oscillation behavior, paving the way for a comprehensive understanding of XOs' performance characteristics. temperature sweep simulations were induced to verify the theory and found that the parameter drift and thermal perturbation are close to the theory we proposed, which can be applied in temperature‐compatible XOs. The significance of this study lies not only in its contribution to the design and implementation of compact footprint XOs in the oscillator circuit platform but also in its provision of experimental evidence for fabricating wide temperature range compensated XO devices. The results show that the capacitance in the equivalent model of a crystal oscillator plays a dominant role in shaping the output waveform and exhibits relatively good temperature stability characteristics and serve as a valuable resource for engineers and researchers working on improving the performance and reliability of XOs, ultimately enabling the development of more advanced and efficient integrated circuits.
Heterogeneous Catalyst Coating for Boosting the Activity and Chromium Tolerance of Cathodes for Solid Oxide Fuel Cells
Authors: Huang Jiongyuan , Liang Fujun , Zhao Sunce , Zhao Ling , Ai Na , Jiang San Ping , Wang Xin , Fang Huihuang , Luo Yu , Chen Kongfa 
Published: February 2024

AbstractA challenge hindering the development of durable solid oxide fuel cells (SOFCs) is the significant performance degradation of cathodes owing to poisoning by volatile Cr originating from the Fe─Cr alloy interconnect. Herein, a heterogeneous catalyst coating, composed of Ba1−xCe0.8Gd0.2O3–δ and BaCO3, remarkably improves the oxygen adsorption, dissociation capability, and Cr resistance of a La0.6Sr0.4Co0.2Fe0.8O3–δ (LSCF) cathode is demonstrated. The coherent heterointerface interactions formed between the catalyst coating and LSCF result in varied levels of surface strain and electrostatic interactions, significantly suppressing Sr surface segregation on LSCF. A single cell with the catalyst coating‐decorated LSCF (CC‐LSCF) achieves a peak power density of 1.73 W cm−2 at 750 °C, with no noticeable performance degradation for 100 h. The CC‐LSCF cathode also exhibits outstanding durability under accelerated Cr poisoning conditions, compared with the tremendous degradation rate of 0.42% h−1 for the bare LSCF cathode. The enhanced Cr resistance is attributed to synergy induced by the stabilization of the lattice Sr cations by heterointerface interactions and the remarkable structural stability of the catalyst coating under Cr poisoning conditions. The novel heterointerface engineering strategy in this study provides insight into the design and development of active and Cr‐tolerant cathodes.
Single Cell Microgels for High‐Throughput Magnetic Sorting and Sequencing of Antigen‐Specific Antibodies
Authors: Chen Jieru , Lin Xiaoxuan , Sun Fang , Wen Huilin , Huang Zhenxiang , Li Wenying , Jiang Le , Yu Ziyi , Hu Chi 
Published: February 2024

AbstractProgress in the discovery of potent antibodies for therapeutic and research purposes has been accelerated by mining the antibody repertoire of plasma cells and plasmablasts. Magnetic activated cell sorting (MACS) is a well recognized method for cell sorting, however, secreted IgGs rapidly diffuse away from cells, therefore requiring specialized technique to compartmentalize individual cells and capture their secretions for MACS isolation. To this end, a scalable method to prepare hydrogel‐based microcontainers for single cell culture and subsequent sorting based on their secreted IgG products at high‐throughput level are described. In situ crosslinkable microgels featuring tunable viscosity are fabricated to act as soft compartments that accommodate delicate primary cells and capture secreted proteins, while providing solid supports after solgel transition to enable immune recognition with chemically functionalized magnetic nanorods in aqueous medium for MACS separation. Splenocytes are sorted based on antigen‐specific IgG production from mice immunized with an antibiotic target ceftriaxone sodium, enriching a population of 220 000 000 to 800 000 prevalence following sorting. This enrichment ratio is substantially higher than conventional fluorescence activated cell sorting (FACS) methods, likely due to the multivalency of the antigen‐conjugated magnetic nanorods.
Semiconductor Nanoplatelets Based Host‐Guest Assembly Structure with High Color Purity for Hue‐Recognizable Lateral Flow Immunoassay
Authors: Zhu Yefeng , Ao Lijiao , Chu Sai , Liao Yuting , Wang Jing , Hu Jun , Huang Liang 
Published: February 2024

AbstractThe hue‐recognition based point‐of‐care diagnosis favorites both human visual perception and imaging analysis with portable terminals. Semiconductor nanoplatelets (NPLs) as an emerging narrow‐emissive fluorophore particularly suit above color‐purity desired diagnosis, but are hindered by their intrinsic hydrophobicity and difficulty in controlled assembly. Here the host‐guest assembling of hydrophobic NPLs are developed within 3D porous space of dendritic templates, and stepwise surface silanization to fabricate silica‐NPLs‐silica structure (SNS). The structure enables high fluorescence of single assembly reaching 246‐fold and 4‐fold brightness of single NPL and commercial europium‐doped polystyrene bead. The strategy is compatible with various colored NPLs (≈540–640 nm emission) and provides stable aqueous colloids with well‐preserved emission. The high brightness and narrow emission of SNS enable both signal amplification and visual/image color discrimination. The hue‐recognizable lateral flow immunoassay for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) nucleocapsid protein reveals an average naked‐eye interpretation accuracy of 92.9% over six concentration levels referring to standard card, accompanied by a quantification linear range of 0.13–200 ng mL−1 and a limit of detection of 0.13 ng mL−1 by smartphone. The diagnostic results for SARS‐CoV‐2 positive clinical samples demonstrate easily distinguishable antigen positive levels based on hue‐gradients, which holds significance for home‐based self‐testing of pathogens.
Realizing High Reversible Zinc Metal Anode by Modulating Surface Chemistry and Crystal Structure
Authors: Wang Zhen , Zhu Xiao , Tao Xiao , Feng Pan , Wang Jianming , Chen Jian 
Published: February 2024

AbstractThe uncontrollable dendrite growth on the Zn metal anode severely deteriorates the capacity and cycling stability of aqueous zinc–ion batteries (AZIBs), thereby retarding their practical application. In this work, through the combination of surface chemistry and crystal structure regulation, a duplex route of plasma sputtering and mechanical stretching is proposed to remove surface passivation layer and increase surface crystal defect (dislocations and textures) of Zn metal anode itself. Plasma sputtering can almost completely remove the surface passivation layer, ensuring a uniform Zn2+ flux at the interface, which is conducive to uniform nucleation. Mechanical stretching can increase the surface dislocation density and (002) texture, the former can reduce Zn2+ nucleation barrier, while the latter can guide Zn to grow parallel to the surface, inhibiting the growth of dendrites. Consequently, the as‐fabricated symmetrical cells exhibit stable and long lifespan (3560 h at 0.5 mA cm−2 for 0.5 mA h cm−2). The assembled full cells with α‐MnO2 cathode deliver a nearly 100% average coulombic efficiency even after 1000 cycles at 5 A g−1. Coupling a surface chemistry regulation with crystal structure control will be enlightening for solve the issues of metallic anodes in advanced metallic‐based batteries.