bluuebunny/crossref_metadata_2025_split huggingface dataset:
Female Sexual Well-Being Scale™ (FSWB Scale™): Development and Psychometric Validation in Sexually Functional Women
Authors: Rosen Raymond C. , Bachmann Gloria A. , Reese Jennifer Barsky , Gentner Louis , Leiblum Sandra , Wajszczuk Charles , Wanser Rita 
Published: March 2009

ABSTRACT

Introduction
No validated instrument exists for measuring female sexual well-being (FSWB™) in women without medical/psychosocial conditions.

Aims
To develop and psychometrically validate a self-administered Female Sexual Well-Being Scale™ (FSWB Scale™) for assessing sexual well-being in sexually functional women.

Methods
Important aspects of FSWB™ were identified via focus groups, debriefing interviews, and administration of an initial scale to 111 US women aged 21–72 years reporting normal sexual function (Female Sexual Function Index &gt;26). Principal components analysis and psychometric validity testing of a FSWB Scale™ were conducted in a second study of 332 women.

Main Outcome Measures
To develop a FSWB Scale, based on qualitative input from women reporting normal sexual function, and determine its factor structure and psychometric validity.

Results
Four aspects of FSWB were identified from the qualitative research. Women’s preferred language to describe sexual well-being and preferred response formats were incorporated into the scale. A principle components analysis of quantitative study data from 332 women aged 21–72 years reporting normal sexual function identified 5 factors with eigenvalues &gt;1: interpersonal domain (6 items), cognitive-emotional domain (5 items), physical arousal domain (3 items), orgasm-satisfaction domain (3 items), and external lubrication domain (2 items). The external lubrication domain did not demonstrate strong positive correlations with the other 4 domains, so it was not retained in the final scale. A high degree of internal consistency was demonstrated for the 4 domains (Cronbach’s alpha values: 0.84–0.92). Test-retest reliability over a 2-week period was high (r &gt; 0.80) or moderately high (r &gt; 0.70) for the 4 domain scores. Correlation coefficients between FSWB Scale domain scores and standardized scale scores for female sexual function, depression, and social desirability demonstrated the construct validity of the FSWB Scale.

Conclusion
A 17-item FSWB Scale was developed and psychometrically validated as a reliable, multidimensional, self-administered instrument for assessing sexual well-being in women of different ages.
THIN‐LAYER DRYING BEHAVIORS OF OLIVE LEAVES (OLEA EUROPAEA L.)
Authors: ERBAY ZAFER , ICIER FILIZ 
Published: March 2009

ABSTRACT Recently, interest in olive leaves has increased because of their high phenolic contents. Although the critical process in olive leaf treatment is drying, the drying behavior of olive leaves has not been known yet in the literature. In this study, olive leaves were dried in a tray drier at the temperature range of 50–70C and velocity range of 0.5–1.5 m/s. Eleven different semitheoretical thin‐layer drying curve models were used to characterize the drying behavior of olive leaves. Effects of drying air temperature and velocity on model coefficients were determined by multiple regression analysis. The correlation coefficient (r), the reduced chi square (χ2) and the root mean square error (RMSE) were taken as the statistical criteria parameters for model comparisons. It was found that the modified Henderson and Pabis model represented drying characteristics best with r = 0.9955. Effective diffusivity was computed by taking account of temperature and air velocity. The temperature‐ and velocity–temperature‐dependent activation energies were 60.97 ± 3.21 and 75.98 kJ/mol, respectively. It was concluded that olive leaves could be dried successfully by thin‐layer drying procedure, and the drying rate was influenced mainly by drying air temperature.PRACTICAL APPLICATIONSOlive trees are important plants covering ∼8 million hectares in the whole world. Recent studies have shown that olive leaves have higher amounts of phenolic contents such as oleuropein and hydroxytyrosol. Oleuropein is especially a very valuable component in food, medicines and in the drug industry.Although the importance of olive leaves has been discussed in several scientific studies, there have been few studies for using olive leaves industrially. Because the main process in olive leaf treatment is drying, modeling and determining the drying behavior is an important step for designing and optimizing the olive leaf treatment. Unfortunately, there has been no study ever carried out explaining the drying behavior of olive leaves in open literature to the best of the authors' knowledge.
Lateral line system of fish
Authors: BLECKMANN Horst , ZELICK Randy 
Published: March 2009

AbstractThe lateral line is a sensory system that allows fishes to detect weak water motions and pressure gradients. The smallest functional unit of the lateral line is the neuromast, a sensory structure that consists of a hair cell epithelium and a cupula that connects the ciliary bundles of the hair cells with the water surrounding the fish. The lateral line of most fishes consists of hundreds of superficial neuromasts spread over the head, trunk and tail fin. In addition, many fish have neuromasts embedded in lateral line canals that open to the environment through a series of pores. The present paper reviews some more recent aspects of the morphology, behavioral relevance and physiology of the fish lateral line. In addition, it reports some new findings with regard to the coding of bulk water flow.
Soundscapes and the sense of hearing of fishes
Authors: FAY Richard 
Published: March 2009

AbstractUnderwater soundscapes have probably played an important role in the adaptation of ears and auditory systems of fishes throughout evolutionary time, and for all species. These sounds probably contain important information about the environment and about most objects and events that confront the receiving fish so that appropriate behavior is possible. For example, the sounds from reefs appear to be used by at least some fishes for their orientation and migration. These sorts of environmental sounds should be considered much like “acoustic daylight,” that continuously bathes all environments and contain information that all organisms can potentially use to form a sort of image of the environment. At present, however, we are generally ignorant of the nature of ambient sound fields impinging on fishes, and the adaptive value of processing these fields to resolve the multiple sources of sound. Our field has focused almost exclusively on the adaptive value of processing species‐specific communication sounds, and has not considered the informational value of ambient “noise.” Since all fishes can detect and process acoustic particle motion, including the directional characteristics of this motion, underwater sound fields are potentially more complex and information‐rich than terrestrial acoustic environments. The capacities of one fish species (goldfish) to receive and make use of such sound source information have been demonstrated (sound source segregation and auditory scene analysis), and it is suggested that all vertebrate species have this capacity. A call is made to better understand underwater soundscapes, and the associated behaviors they determine in fishes.
Secondary neurogenesis and telencephalic organization in zebrafish and mice: a brief review
Authors: WULLIMANN Mario F. 
Published: March 2009

AbstractMost zebrafish neurodevelopmental studies have focused on the embryo, which is characterized by primary neurogenesis of mostly transient neurons. Secondary neurogenesis becomes dominant in the hatching larva, when major brain parts are established and begin to differentiate. This developmental period allows for a comparative analysis of zebrafish brain organization with amniotes at equivalent stages of neurogenesis. Within a particular time window, the early forebrains of mice (Embyronic stage [E] 12.5/13.5 days [d]) and zebrafish (3 d) reveal highly comparable expression patterns of genes involved in neurogenesis, for example proneural and other transcription factors (Neurogenin1, NeuroD, Mash1/Zashla and Pax6). Further topological correspondences are seen in the expression of LIM and homeobox genes, such as Lhx6/7, Tbr2 and Dlx2a. When this analysis is extended to gamma‐aminobutyric acid/glutamic acid decarboxylase (GABA/GAD) cell patterns during this critical time window, an astonishing degree of similarity between the two species is again seen, for example regarding the presence of GABA/GAD cells in the subpallium, with the pallium only starting to be invaded by such cells from the subpallium. Furthermore, the expression of proneural and other genes correlates with GABA cell patterns (e.g. Mash1/Zash1a gene expression in GABA‐positive and Neurogenin1/NeuroD in GABA‐negative telencephalic regions) in mice and zebrafish. Data from additional vertebrates, such as Xenopus, are also highly consistent with this analysis. Therefore, the vertebrate forebrain appears to undergo a phylotypic stage of secondary neurogenesis, characterized by regionally separated GABAergic (inhibitory) versus glutamatergic (excitatory) cell production sites, which are obscured later in development by tangential migration. This period is highly advantageous for molecular neuroanatomical cross‐species comparisons.
Oculometry findings in high myopia at adult age: considerations based on oculometric follow‐up data over 28 years in a cohort‐based Danish high‐myopia series
Authors: Fledelius Hans C. , Goldschmidt Ernst 
Published: March 2009

Abstract.Purpose:  To present and discuss oculometry data in a series of adults with high myopia followed between the ages of 26 and 54 years. Emphasis is on axial length (AL) findings and corneal curvature radius (Crad).Methods:  Thirty‐four out of the 39 individuals recruited as teenagers from a Copenhagen 1948 birth cohort with myopia of at least 6 D have had current follow‐up exams, to include AL measurements (by ultrasound, 1974–2002; the latter year also with the Zeiss IOLMaster) and keratometry. The cross‐sectional and longitudinal analyses are based primarily on the eyes with high myopia; however, the fellow eye is also assessed in unilateral cases.Results:  At age 54 years, the maximum myopia in the series was −26 D; the highest AL value was 35.4 mm. The myopia had increased in most, with an increase from the 26‐year oculometry baseline averaging 1.0 D [standard deviation (SD) 1.84]. Ultrasound measurements over the 28 years gave a significant correlation between axial eye elongation and myopia progression of adult age (r = 0.65). The regression line was y = 0.43 + 0.36x, with myopia increase on the x‐axis. Throughout sessions, the association between AL and refraction was given by correlation coefficients numerically above 0.8, whereas AL and Crad had r‐values of 0.3–0.5. However, a mean Crad in the sample of 7.66 (SD 0.28) mm meant that the more general expectancy of rather flat corneas in high myopia was not fulfilled. Our data further suggested a reduction in lens power over the study period.Conclusion:  In relation to refraction, AL and Crad remain the two main oculometry parameters. Apparently the correlation patterns regarding the cornea that are broadly valid for axial ametropia in the population cannot be extended to the marginal high myopia tail of the distribution. A significant proportion of eyes with high myopia thus had steeper corneas than expected, as a so‐called index contribution (albeit a small one) to the marginal refractive error.
Strong Smooth Muscle Differentiation Is Dependent on Myocardin Gene Amplification in Most Human Retroperitoneal Leiomyosarcomas
Authors: Pérot Gaëlle , Derré Josette , Coindre Jean-Michel , Tirode Franck , Lucchesi Carlo , Mariani Odette , Gibault Laure , Guillou Louis , Terrier Philippe , Aurias Alain 
Published: March 2009

Abstract
Myocardin (MYOCD), a serum response factor (SRF) transcriptional cofactor, is essential for cardiac and smooth muscle development and differentiation. We show here by array-based comparative genomic hybridization, fluorescence in situ hybridization, and expression analysis approaches that MYOCD gene is highly amplified and overexpressed in human retroperitoneal leiomyosarcomas (LMS), a very aggressive well-differentiated tumor. MYOCD inactivation by shRNA in a human LMS cell line with MYOCD locus amplification leads to a dramatic decrease of smooth muscle differentiation and strongly reduces cell migration. Moreover, forced MYOCD expression in three undifferentiated sarcoma cell lines and in one liposarcoma cell line confers a strong smooth muscle differentiation phenotype and increased migration abilities. Collectively, these results show that human retroperitoneal LMS differentiation is dependent on MYOCD amplification/overexpression, suggesting that in these well-differentiated LMS, differentiation could be a consequence of an acquired genomic alteration. In this hypothesis, these tumors would not necessarily derive from cells initially committed to smooth muscle differentiation. These data also provide new insights on the cellular origin of these sarcomas and on the complex connections between oncogenesis and differentiation in mesenchymal tumors. [Cancer Res 2009;69(6):2269–78]
Quantitative Phosphoproteomics Reveals a Cluster of Tyrosine Kinases That Mediates Src Invasive Activity in Advanced Colon Carcinoma Cells
Authors: Leroy Cédric , Fialin Camille , Sirvent Audrey , Simon Valérie , Urbach Serge , Poncet Joël , Robert Bruno , Jouin Patrick , Roche Serge 
Published: March 2009

Abstract
The nonreceptor tyrosine kinase Src is frequently overexpressed and/or activated in human colorectal carcinoma (CRC), and its increased activity has been associated with a poor clinical outcome. Src has been implicated in growth and invasion of these cancer cells by still not well-known mechanisms. Here, we addressed Src oncogenic signaling using quantitative phosphoproteomics. Src overexpression increased growth and invasiveness of metastatic SW620 CRC cells. Stable isotope labeling with amino acids in cell culture in combination with liquid chromatography tandem mass spectrometry allowed the identification of 136 proteins which exhibited a significant increase in and/or association with tyrosine phosphorylation upon Src expression. These mainly include signaling, cytoskeleton, and vesicular-associated proteins. Interestingly, Src also phosphorylated a cluster of tyrosine kinases, i.e., the receptors Met and EphA2, the cytoplasmic tyrosine kinase Fak, and pseudo-tyrosine kinase SgK223, which were required for its invasive activity. Similar results were obtained with metastatic Colo205 CRC cells that exhibit high endogenous Src activity. We concluded that Src uses a tyrosine kinases network to promote its invasive activity in CRC and this implicates a reverse signaling via tyrosine kinase receptors. Targeting these tyrosine kinases may be of significant therapeutic value in this cancer. [Cancer Res 2009;69(6):2279–86]
CYP1A1/2 Haplotypes and Lung Cancer and Assessment of Confounding by Population Stratification
Authors: Aldrich Melinda C. , Selvin Steve , Hansen Helen M. , Barcellos Lisa F. , Wrensch Margaret R. , Sison Jennette D. , Kelsey Karl T. , Buffler Patricia A. , Quesenberry Charles P. , Seldin Michael F. , Wiencke John K. 
Published: March 2009

Abstract
Prior studies of lung cancer and CYP1A1/2 in African-American and Latino populations have shown inconsistent results and have not yet investigated the haplotype block structure of CYP1A1/2 or addressed potential population stratification. To investigate haplotypes in the CYP1A1/2 region and lung cancer in African-Americans and Latinos, we conducted a case-control study (1998–2003). African-Americans (n = 535) and Latinos (n = 412) were frequency matched on age, sex, and self-reported race/ethnicity. We used a custom genotyping panel containing 50 single nucleotide polymorphisms in the CYP1A1/2 region and 184 ancestry informative markers selected to have large allele frequency differences between Africans, Europeans, and Amerindians. Latinos exhibited significant haplotype main effects in two blocks even after adjusting for admixture [odds ratio (OR), 2.02; 95% confidence interval (95% CI), 1.28–3.19 and OR, 0.55; 95% CI, 0.36–0.83], but no main effects were found among African-Americans. Adjustment for admixture revealed substantial confounding by population stratification among Latinos but not African-Americans. Among Latinos and African-Americans, interactions between smoking level and haplotypes were not statistically significant. Evidence of population stratification among Latinos underscores the importance of adjusting for admixture in lung cancer association studies, particularly in Latino populations. These results suggest that a variant occurring within the CYP1A2 region may be conferring an increased risk of lung cancer in Latinos. [Cancer Res 2009;69(6):2340–8]
Cetuximab Attenuates Metastasis and u-PAR Expression in Non–Small Cell Lung Cancer: u-PAR and E-Cadherin are Novel Biomarkers of Cetuximab Sensitivity
Authors: Nikolova Dessislava A. , Asangani Irfan A. , Nelson Laura D. , Hughes Dennis P.M. , Siwak Doris R. , Mills Gordon B. , Harms Andrea , Buchholz Erika , Pilz Lothar R. , Manegold Christian , Allgayer Heike 
Published: March 2009

AbstractCetuximab, which blocks ligand binding to epidermal growth factor receptor (EGFR), is currently being studied as a novel treatment for non–small cell lung cancer (NSCLC). However, its mechanisms of action toward metastasis, and markers of drug sensitivity, have not been fully elucidated. This study was conducted to (a) determine the effect of Cetuximab on invasion and NSCLC-metastasis; (b) investigate urokinase-type plasminogen activator receptor (u-PAR), a major molecule promoting invasion and metastasis, as a target molecule; (c) delineate molecular mediators of Cetuximab-induced metastasis inhibition; and (d) identify biomarkers of drug sensitivity in NSCLC. Cetuximab treatment resulted in reduced growth and Matrigel invasion of H1395 and A549 NSCLC cell lines, in parallel with reduced u-PAR mRNA and protein. u-PAR down-regulation was brought about by suppressing the binding of JunD and c-Jun to u-PAR promoter motif −190/−171 in vivo, and an inhibition of MAP/ERK kinase signaling. Furthermore, Cetuximab inhibited NSCLC proliferation and metastasis to distant organs in vivo as indicated by the chicken embryo metastasis assay. Low E-cadherin and high u-PAR, but not EGFR, was associated with resistance to Cetuximab in seven NSCLC cell lines. Furthermore, siRNA knockdown of u-PAR led to a resensitization to Cetuximab. Moreover, low E-cadherin and high u-PAR was found in 63% of resected tumor tissues of NSCLC patients progressing under Cetuximab therapy. This is the first study to show u-PAR as a target and marker of sensitivity to Cetuximab, and to delineate novel mechanisms leading to metastasis suppression of NSCLC by Cetuximab. [Cancer Res 2009;69(6):2461–70]
Mitochondrial Dysfunction and Reactive Oxygen Species Imbalance Promote Breast Cancer Cell Motility through a CXCL14-Mediated Mechanism
Authors: Pelicano Helene , Lu Weiqin , Zhou Yan , Zhang Wan , Chen Zhao , Hu Yumin , Huang Peng 
Published: March 2009

Abstract
Although mitochondrial dysfunction and reactive oxygen species (ROS) stress have long been observed in cancer cells, their role in promoting malignant cell behavior remains unclear. Here, we show that perturbation of the mitochondrial respiratory chain in breast cancer cells leads to a generation of subclones of cells with increased ROS, active proliferation, high cellular motility, and invasive behaviors in vitro and in vivo. Gene expression analysis using microarrays revealed that all subclones overexpressed CXCL14, a novel chemokine with undefined function. We further show that CXCL14 expression is up-regulated by ROS through the activator protein-1 signaling pathway and promotes cell motility through elevation of cytosolic Ca2+ by binding to the inositol 1,4,5-trisphosphate receptor on the endoplasmic reticulum. Abrogation of CXCL14 expression using a decoy approach suppressed cell motility and invasion. Our data suggest that mitochondrial dysfunction and ROS stress promote cancer cell motility through a novel pathway mediated by CXCL14. [Cancer Res 2009;69(6):2375–83]
Associations between Selected Biomarkers and Prognosis in a Population-Based Pancreatic Cancer Tissue Microarray
Authors: Takikita Mikiko , Altekruse Sean , Lynch Charles F. , Goodman Mark T. , Hernandez Brenda Y. , Green Mark , Cozen Wendy , Cockburn Myles , Sibug Saber Maria , Topor Marie , Zeruto Chris , Abedi-Ardekani Behnoush , Reichman Marsha E. , Hewitt Stephen M. 
Published: March 2009

Abstract
Pancreatic cancer is the fourth leading cause of cancer death in the United States. Prognostic biomarkers are lacking, and treatment has limited effect on survival. Tissues from Surveillance, Epidemiology, and End Results registries (Iowa, Hawaii, and Los Angeles) were used to build a tissue microarray of 161 pancreatic tumors (113 resections and 48 biopsies). Proportional hazard models adjusted for age, race, sex, stage, time-period of diagnosis, and treatment. Associations were examined between markers (MUC1, MUC2, MUC5AC, synaptophysin, chromogranin, neuron specific enolase, epidermal growth factor receptor, HER2, CD5, CD138, CK5/6, CK19, CK20, and p53) and survival time from diagnosis. After adjusting for covariates, borderline statistically significant associations were seen between expression of each of the three mucins (MUC1, MUC2, and MUC5AC) and shorter survival time. The associations strengthened for 154 (96%) adenocarcinomas, particularly the 120 (75%) well-differentiated to moderately differentiated ductal adenocarcinomas, a tumor type that occurred more often in the cohort among White cases than cases of other racial origin (P &lt; 0.01). For differentiated ductal adenocarcinomas, associations with shorter survival time were seen for expression of all three mucins combined versus other mucin expression patterns (adjusted hazard ratio, 1.8; 95% confidence interval, 1.2–2.6) and for MUC2(+) versus MUC2(−) expression (adjusted hazard ratio, 1.6; 95% confidence interval, 1.1–2.4). Mucin gene expression, particularly MUC2 expression, may have prognostic value for differentiated adenocarcinomas. Tumor histologies differed in this and Japanese cohorts. The tissue microarray is available to evaluate other biomarkers. Tissue-based surveillance can be used to monitor tumor histology in populations and facilitate applied research. [Cancer Res 2009;69(7):2950–5]