bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncovering the antifungal activities of wild apple-associated bacteria against two canker-causing fungi, Cytospora mali and C. parasitica
Authors: Bozorov Tohir A. , Toshmatov Zokir O. , Kahar Gulnaz , Muhammad Surayya M. , Liu Xiaojie , Zhang Daoyuan , Aytenov Ilkham S. , Turakulov Khurshid S. 
Published: March 2024

AbstractCytospora canker has become a devastating disease of apple species worldwide, and in severe cases, it may cause dieback of entire trees. The aim of this study was to characterize the diversity of cultivable bacteria from the wild apple microbiota and to determine their antifungal ability against the canker-causing pathogenic fungi Cytospora mali and C. parasitica. Five bacterial strains belonging to the species Bacillus amyloliquefaciens, B. atrophaeus, B. methylotrophicus, B. mojavensis, and Pseudomonas synxantha showed strong antagonistic effects against pathogenic fungi. Therefore, since the abovementioned Bacillus species produce known antifungal compounds, we characterized the antifungal compounds produced by Ps. synxantha. Bacteria grown on nutritional liquid medium were dehydrated, and the active compound from the crude extract was isolated and analysed via a range of chromatographic processes. High-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance analyses revealed a bioactive antifungal compound, phenazine-1-carboxylic acid (PCA). The minimum inhibitory concentration (MIC) demonstrated that PCA inhibited mycelial growth, with a MIC of 10 mg mL−1. The results suggested that PCA could be used as a potential compound to control C. mali and C. malicola, and it is a potential alternative for postharvest control of canker disease.
Identification of membrane curvature sensing motifs essential for VPS37A phagophore recruitment and autophagosome closure
Authors: Ye Yansheng , Liang Xinwen , Wang Guifang , Bewley Maria C. , Hamamoto Kouta , Liu Xiaoming , Flanagan John M. , Wang Hong-Gang , Takahashi Yoshinori , Tian Fang 
Published: March 2024

AbstractVPS37A, an ESCRT-I complex component, is required for recruiting a subset of ESCRT proteins to the phagophore for autophagosome closure. However, the mechanism by which VPS37A is targeted to the phagophore remains obscure. Here, we demonstrate that the VPS37A N-terminal domain exhibits selective interactions with highly curved membranes, mediated by two membrane-interacting motifs within the disordered regions surrounding its Ubiquitin E2 variant-like (UEVL) domain. Site-directed mutations of residues in these motifs disrupt ESCRT-I localization to the phagophore and result in defective phagophore closure and compromised autophagic flux in vivo, highlighting their essential role during autophagy. In conjunction with the UEVL domain, we postulate that these motifs guide a functional assembly of the ESCRT machinery at the highly curved tip of the phagophore for autophagosome closure. These results advance the notion that the distinctive membrane architecture of the cup-shaped phagophore spatially regulates autophagosome biogenesis.
Foundation model for cancer imaging biomarkers
Authors: Pai Suraj , Bontempi Dennis , Hadzic Ibrahim , Prudente Vasco , Sokač Mateo , Chaunzwa Tafadzwa L. , Bernatz Simon , Hosny Ahmed , Mak Raymond H. , Birkbak Nicolai J. , Aerts Hugo J. W. L. 
Published: March 2024

AbstractFoundation models in deep learning are characterized by a single large-scale model trained on vast amounts of data serving as the foundation for various downstream tasks. Foundation models are generally trained using self-supervised learning and excel in reducing the demand for training samples in downstream applications. This is especially important in medicine, where large labelled datasets are often scarce. Here, we developed a foundation model for cancer imaging biomarker discovery by training a convolutional encoder through self-supervised learning using a comprehensive dataset of 11,467 radiographic lesions. The foundation model was evaluated in distinct and clinically relevant applications of cancer imaging-based biomarkers. We found that it facilitated better and more efficient learning of imaging biomarkers and yielded task-specific models that significantly outperformed conventional supervised and other state-of-the-art pretrained implementations on downstream tasks, especially when training dataset sizes were very limited. Furthermore, the foundation model was more stable to input variations and showed strong associations with underlying biology. Our results demonstrate the tremendous potential of foundation models in discovering new imaging biomarkers that may extend to other clinical use cases and can accelerate the widespread translation of imaging biomarkers into clinical settings.
Ammonium-rich bright areas on Ceres demonstrate complex chemical activity
Authors: De Sanctis Maria Cristina , Ammannito E. , Carrozzo F. G. , Ciarniello M. , De Angelis S. , Ferrari M. , Frigeri A. , Raponi A. 
Published: March 2024

AbstractThe dwarf planet Ceres has been proven to be a world with an extraordinary diversity of chemical species formed in aqueous environments. Dantu crater, being one of the largest and deepest impact craters on Ceres, offers valuable insights into the geological history and composition of this enigmatic world. Its particular position, within a topographic low, can be considered a window into the composition of Ceres’s subsurface. One of the intriguing aspects of Dantu is the presence of several bright areas called “faculae”. These bright materials, distributed unevenly on the Cerean surface, are believed to be residua from salty fluids, likely still circulating in the subsurface and capable to extrude onto the surface, as demonstrated by the identification of fresh hydrohalite (NaCl·2(H2O)) on the bright faculae in another exceptional crate, Occator. The detailed investigation of the Dantu’s faculae reveals the presence of at least two different “populations” of bright materials, compositionally distinct even if in close geographical proximity. These faculae appear different, white and yellow, in the color images (RGB: R = 0.917 μm, G = 0.653 μm, B = 0.438 μm) taken by the Dawn camera. The spectra show that the first population -white- is mainly composed of sodium carbonate, similar to other identified bright areas on Ceres; the second one -yellow- is likely dominated by ammonium-rich components. The spectra of the yellow faculae show specific and clear bands, associated to ammonium bearing phases, but the exact species producing such spectral features are not unambiguously identified. A potential candidate is ammonium bicarbonates, but we cannot exclude other compounds and combinations of different species. Other occurrences of these yellow faculae have been identified in a few other areas, even if less numerous and with a much smaller extent with respect to the Dantu crater. The discovery of a very bright component different from the carbonates and salts previously identified increases the variety and complexity of the salty aqueous solutions on Ceres, offering tantalizing clues about the potential for habitable environments and subsurface fluid reservoirs on this dwarf planet.
MemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
Authors: Reinhard John , Starke Leonhard , Klose Christian , Haberkant Per , Hammarén Henrik , Stein Frank , Klein Ofir , Berhorst Charlotte , Stumpf Heike , Sáenz James P , Hub Jochen , Schuldiner Maya , Ernst Robert 
Published: March 2024

AbstractBiological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work has provided insights into the subcellular distribution of lipids in the yeastSaccharomyces cerevisiae, but the composition of the endoplasmic reticulum (ER) membrane, which also crucially regulates lipid metabolism and the unfolded protein response, remains insufficiently characterized. Here, we describe a method for purifying organelle membranes from yeast, MemPrep. We demonstrate the purity of our ER membrane preparations by proteomics, and document the general utility of MemPrep by isolating vacuolar membranes. Quantitative lipidomics establishes the lipid composition of the ER and the vacuolar membrane. Our findings provide a baseline for studying membrane protein biogenesis and have important implications for understanding the role of lipids in regulating the unfolded protein response (UPR). The combined preparative and analytical MemPrep approach uncovers dynamic remodeling of ER membranes in stressed cells and establishes distinct molecular fingerprints of lipid bilayer stress.
Reliability and Feasibility of Administering a Child Language Assessment via Telehealth
Authors: Campbell Deborah R. , Lawrence Jennella E. , Goldstein Howard 
Published: March 2024

Purpose:
Despite the speed with which telehealth use advanced during the COVID-19 pandemic, evidence is needed to support the remote delivery of standardized assessments. This study investigated the reliability and feasibility of administering a standardized language assessment administered in real-world telehealth scenarios compared to in-person administration.

Method:
A total of 100 children between the ages of 3 and 12 years were administered one of three versions of the Clinical Evaluation of Language Fundamentals (CELF). Children were administered the CELF by the same licensed speech-language pathologists (SLPs) in person and using telehealth, with the order counterbalanced. Means for Core Language standard scores were compared between conditions and among devices. Descriptive statistics summarized the behavior and technology disruptions during administration as well as the results of parent and SLP telehealth perception surveys.

Results:
In-person and telehealth mean scores on all three versions of the CELF revealed no systematic differences of one condition under- or overestimating another. The incidence of child behavior disruptions was similar for both test administration conditions. Adaptations compensated for the rare technology disruptions. Despite no significant language score and behavior differences between testing conditions, parents reported they continued to prefer in-person assessments. SLP participants viewed telehealth overall positively but identified conditions in which they continued to prefer in-person delivery.

Conclusions:
This study provides evidence of minimal or no differences in scores and behavioral or technological disruptions between remote and in-person administration of the CELF core language assessments. SLP and parent participants' attitudes toward remote delivery of standardized tests appear to be evolving in a positive direction compared to previous studies.

Supplemental Material:

https://doi.org/10.23641/asha.25292752
3sG: Three‐stage guidance for indoor human action recognition
Authors: Nan Hai , Ye Qilang , Yu Zitong , An Kang 
Published: March 2024

AbstractInference using skeleton to steer RGB videos is applicable to fine‐grained activities in indoor human action recognition (IHAR). However, existing methods that explore only spatial alignment are prone to bias, resulting in limited performance. The authors propose a Three‐stage Guidance (3sG) framework, leveraging skeleton knowledge to promote RGB in three stages. First, a soft shading image is proposed for alleviating background noise in videos, allowing the network to directly focus more on the motion region. Second, the authors propose to extract RGB frames of interest to reduce the computational effort. Furthermore, to explore more fully the complementary information between skeletons and RGB, the skeleton is coupled to the frame representation in a different spatial–temporal sharing pattern. Third, the global skeleton and skeleton‐guided RGB features are fed into the shared classifiers, which approximate the logit distributions of the two to enhance the performance in RGB unimodal. Finally, a fusion strategy that utilizes two learnable parameters to adaptively integrate the skeleton with the RGB is proposed. 3sG outperforms the state‐of‐the‐art results on the Toyota Smarthome dataset while it is more efficient than similar methods on the NTU RGB+D dataset.
Implication des patients dans l’évaluation multisource : élaboration et évaluation d’un outil de rétroaction destiné aux résidents en médecine familiale
Authors: Dallé Lysiane , Gosselin Isabelle 
Published: March 2024

Contexte : Le comité de compétences du programme de résidence de médecine familiale de l’Université de Montréal planifie l’introduction des patients dans l’évaluation multisource. Plusieurs études ont mis en lumière la valeur pédagogique de cette évaluation, mais les étudiants impliqués ont soulevé plusieurs enjeux liés à la rétroaction obtenue par les patients. Aucune étude ne s’est encore penchée sur la perspective des résidents en médecine familiale. Buts : Examiner les enjeux identifiés par nos résidents en médecine familiale face à l’utilisation d’une fiche de rétroaction conçue pour impliquer les patients dans l’évaluation multisource. Méthodes : Une fiche de rétroaction pour les patients a été testée durant trois mois par 13 résidents de médecine familiale dans trois cliniques du réseau de l’Université de Montréal. Nous avons ensuite tenu des groupes de discussion focalisée et avons procédé à une analyse thématique descriptive. Résultats : Un total de 30 patients ont rempli notre fiche de rétroaction (taux de réponse de 26 %). Les résidents ont jugé la rétroaction des patients pertinente. Les principaux risques soulevés étaient l’utilisation de l’évaluation à des fins de sanction et l’impact sur le lien thérapeutique. La principale barrière identifiée était la difficulté à recruter des patients. Une liste de 13 solutions a été proposée pour l’introduction des patients dans l’évaluation multisource. Conclusion : Les résultats permettront de peaufiner la fiche de rétroaction et faciliter son intégration dans le programme de résidence. La participation des patients à la formation des médecins de famille pourrait leur offrir une opportunité concrète d’améliorer la qualité des soins.
Musik trifft Medizin: eine kraftvolle Verbindung für die Gesundheit
Authors: Pirlet-Berninger Ursula 
Published: March 2024

SummaryIn der Medizin wird Musik bei vielen Indikationen erfolgreich eingesetzt, etwa bei Herz-Kreislauf-Erkrankungen, zur Linderung von Schmerzen, bei neurologischen Störungen oder im Umgang mit Frühgeborenen. Auch bei der Stressbewältigung sowie zur Steigerung von Wohlbefinden und Entspannung kommt Musik zum Einsatz. Positive Effekte haben sowohl das Hören von Musik als auch das eigenständige Musizieren. Das große Therapieangebot der klassischen Musiktherapie bietet ein breites Spektrum spezieller therapeutischer Instrumente und deren Einsatzmöglichkeiten. Verschiedene Fallbeispiele zeigen exemplarisch, wie das Klavierspielen Patientinnen und Patienten bei der Krankheitsbewältigung helfen kann.
Long-term Follow-Up and Regeneration of Retinal Pigment Epithelium (RPE) after Tears of the Epithelium in Exudative Age-Related Macular Degeneration (AMD)
Authors: Skalicky Nicolas , Hatz-Wurziger Katja 
Published: March 2024

Abstract
Background The goals of this study are to evaluate potential long-term visual deterioration associated with retinal pigment epithelial (RPE) tears in patients with neovascular age-related macular degeneration (nAMD) and to find treatment-related and morphological factors that might influence the outcomes.
Patients and Methods This retrospective study enrolled 21 eyes of 21 patients from the database of Vista Eye Clinic Binningen, Switzerland, diagnosed with RPE tears, as confirmed by spectral domain optical coherence tomography (SD-OCT), with a minimum follow-up period of 12 months. Treatment history before and after RPE rupture with anti-VEGF therapy, visual acuity, and imaging (SD-OCT) were analyzed and statistically evaluated for possible correlations.
Results Mean patient age was 80.5 ± 6.2 years. The mean length of total follow-up was 39.7 ± 13.9 months. The mean pigment epithelial detachment (PED) height increased by 363.8 ± 355.5 µm from the first consultation to 562.8 ± 251.5 µm at the last consultation prior to rupture. Therefore, a higher risk of RPE rupture is implied as a result of an increase in PED height (p = 0.004, n = 14). The mean visual acuity before rupture was 66.2 ± 16.0 letters. Mean visual acuity deteriorated to 60.8 ± 18.6 letters at the first consultation after rupture (p = 0.052, n = 21). A statistically nonsignificant decrease in vision was noted in the follow-up period. After 2 years, the mean BCVA decreased by 10.5 ± 23.7 ETDRS letters (p = 0.23, n = 19). PED characteristics before rupture and amount of anti-VEGF injections after rupture did not affect the visual outcome. None of the 21 patients included in our study showed a visual improvement in the long-term follow-up. RPE atrophy
increased significantly from 3.35 ± 2.94 mm2 (baseline) to 6.81 ± 6.25 mm2 over the course of 2 years (p = 0.000 013, n = 20).
Conclusions The overall mean vision decrease after rupture was without statistical significance. There was no significant change in BCVA at the 2-year follow-up, independent of the amount of anti-VEGF injections provided. In this study, there was a significant increase in RPE defect over a follow-up of 2 years, implying progression of contraction of RPE and/or macular atrophy.
Wenn der Körper singt: Atem, Stimme und Bewegung
Authors: Kraus-Geiges Stephanie , McMurtrie Imke 
Published: March 2024

SummaryDie Stimme verleiht Menschen Ausdruckskraft. In speziellen Seminaren werden Körper und Stimme trainiert, um Entspannung und eine achtsame Zuwendung zu sich selbst zu fördern. Die Klangwellen der Stimme haben das Potenzial, die Körpersysteme, vom muskuloskelettalen bis hin zum Hormon- und Nervensystem, in Harmonie zu bringen. Erste Untersuchungen stellen einen Zusammenhang zwischen Stimme und Herzratenvariabilität her.
Power of Life Dance – Lebenslust oder körperorientierte Psychotherapie?
Authors: Böttcher Cornelia 
Published: March 2024

SummaryTanz hat eine heilsame Wirkung auf die Psyche und den Körper. In Studien wurden positive Effekte bei Depressionen, Autismus, schizophrenen und somatoformen Störungen sowie eine Verbesserung der Symptomatik bei chronischen Schmerzen nachgewiesen. Beim Tanzen werden im Zusammenspiel mit der Musik Gefühle angesprochen und über den Körper ausgedrückt. Power of Life Dance (POL) ist eine Methode, die sich diese Effekte zunutze macht. Durch freie Bewegung zur Musik ohne erlernte Tanzschritte oder feste Muster wird die Lebensfreude angesprochen und tiefgreifende Heilungsprozesse werden in Gang gesetzt. POL kommt unter anderem in der Persönlichkeitsentwicklung, im Teambuilding, aber auch bei Patient*innen mit psychischen Störungen oder Schmerzen zum Einsatz.
Musiktherapie im chinesischen Kulturraum
Authors: Mastnak Wolfgang 
Published: March 2024

SummarySetzt man sich mit Musiktherapie im chinesischen Kulturraum auseinander, so stellt sich zunächst die Frage, was damit gemeint ist. Denn das Feld ist extrem facettenreich und umfasst traditionelle Musik in westlich ausgerichteten Kliniken ebenso wie musiktherapeutische Modelle, die auf TCM oder daoistischen Weisheiten beruhen. Zudem gibt es eine Fülle musikbasierter Heilriten ethnischer Minoritäten oder die musiktherapeutische Förderung sozialer Integration und Persönlichkeitsentwicklung chinesischer Kinder in Deutschland. Dies alles systematisch aufzuarbeiten, würde Bände füllen. Deshalb beleuchtet dieser Beitrag repräsentative Modelle und Praktiken, die eine Idee vom Wesen „chinesischer Musiktherapie“ vermitteln sollen.
Digestive neo-epithelialization after endoscopic stenting for upper digestive tract complete disunion
Authors: Ouazzani Sohaib , Lemmers Arnaud , Gonzalez Jean-Michel , Closset Jean , El Moussaoui Imad , Devière Jacques , Barthet Marc 
Published: March 2024

Abstract
Background Complete digestive disunion due to anastomotic necrosis is considered a contraindication to endoscopic repair. However, recent publications have suggested that endoscopic treatment by insertion of a self-expandable metal stent (SEMS) is possible. The report of this patient series aims to demonstrate the use of endoscopic management in selected cases with complete digestive disunion.
Methods Seven consecutive patients with complete and circumferential upper gastrointestinal anastomotic disunion were treated at two European tertiary care centers between 2009 and 2021 by endoscopic insertion of an SEMS. Treatment was performed with a therapeutic gastroscope under general anesthesia, carbon dioxide insufflation, and fluoroscopic guidance, after surgical or percutaneous drainage.
Results All patients were successfully treated by endoscopic insertion of fully or partially covered SEMS left in place for a median of 8 weeks, with a median of 3 endoscopic sessions. Digestive neo-epithelialization was associated with a restored circumferential gut lumen in all cases. The rate of stent migration was 23% and three patients (43%) experienced symptomatic strictures, which were successfully treated by endoscopic dilation.
Conclusion Complete digestive rupture could be successfully treated by endoscopy in selected cases, adding proof-of-concept data regarding guided tissue regeneration alongside SEMS placement.
NHC-Mediated Photochemical/Electrochemical Synthesis of Carbonyl Compounds
Authors: Tang Hai-Tao , Yu Hao-Ran , Pan Ying-Ming , Cui Fei-Hu 
Published: March 2024

AbstractGreat progress has been made in the photochemical/electrochemical reactions of carbonyl compounds catalyzed by N-heterocyclic carbenes (NHCs), allowing for many incredible reactions. The form of active intermediates hugely varies in different reactions. Under light or electricity, different active intermediates can be generated during NHC-catalyzed reactions depending on their interaction with different substrates and the reaction conditions. Intermediates can be converted into α,β-unsaturated acylazoliums, Breslow intermediates, homoenolates, and acylazoliums and subsequently undergo single- or double-electron conversions. This study reviews the different active intermediates formed by NHC in photochemical/electrochemical catalysis.1	Introduction2	NHC-Mediated Photoreactions3	NHC-Mediated Electrochemical Reactions4	Conclusion and Prospects
Bioorthogonal Chemistry at Radboud University: Past, Present and Future
Authors: Rutjes Floris P. J. T. , Bonger Kimberly M. , Neumann Kevin 
Published: March 2024

AbstractOver the past two decades, bioorthogonal chemistry has profoundly impacted various chemistry-related fields, including chemical biology and drug delivery. This transformative progress stems from collaborative efforts involving chemists and biologists, underscoring the importance of interdisciplinary research. In this Account, we present the developments in bioorthogonal chemistry within our Institute for Molecules and Materials at Radboud University. The chemistry disclosed here spans from strained alkynes and alkenes to drug release and bioconjugation strategies, mirroring the extensive scope provided by bioorthogonal chemistry. By reflecting on the chemistry originating at Radboud University, this Account emphasizes that teamwork is essential for driving significant progress in bioorthogonal chemistry.1	Introduction2	Providing BCN as a Robust Bioorthogonal Tool for Chemical Biology and Beyond3	Towards Readily Available Click-to-Release trans-Cyclooctenes4	Giving Molecules Guidance5	Next Generation of Bioconjugation Strategies: Dynamic Click Chemistry6	Conclusions