bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stratocumulus Precipitation Properties Over the Southern Ocean Observed From Aircraft During the SOCRATES Campaign
Authors: Kang L. , Marchand R. T. , Wood R. 
Published: March 2024

AbstractPrecipitation plays an important role in cloud and aerosol processes over the Southern Ocean (SO). The main objective of this study is to characterize SO precipitation properties associated with SO stratocumulus clouds. We use data from the Southern Ocean Clouds Radiation Aerosol Transport Experimental Study (SOCRATES), and leverage observations from airborne radar, lidar, and in situ probes. We find that for the cold‐topped clouds (cloud‐top‐temperature <0°C), the phase of precipitation with reflectivity >0 dBZ is predominantly ice, while reflectivity < −10 dBZ is predominantly liquid. Liquid‐phase precipitation properties are retrieved where radar and lidar are zenith‐pointing. Power‐law relationships between reflectivity (Z) and rain rate (R) are developed, and the derived Z–R relationships show vertical dependence and sensitivity to the presence of droplets with diameters between 10 and 40 μm. Using derived Z–R relationships, a reflectivity‐velocity (ZV) retrieval method, and a radar‐lidar retrieval method, we derive rain rate and other precipitation properties. The retrieved rain rate from all three methods shows good agreement with in‐situ aircraft estimates, with rain rates typically being quite light (<0.1 mm hr−1). We examine the vertical distribution of precipitation properties, and find that rain rate, precipitation number concentration, and precipitation liquid water all decrease as one gets closer to the surface, while precipitation size and distribution width increases. We also examine how cloud base rain rate (RCB) depends on cloud depth (H) and aerosol concentration (Na) for particles with a diameter greater than 70 nm, and find that RCB is proportional to .
Multiple Evolution Modes of Megaripples in the Qaidam Basin and Implications for Ripple‐Like Aeolian Landforms on Mars
Authors: Li Chao , Zhang Zhi , Yu Lupeng , Chen Guoxiang , Yang Junhuai , Dong Zhibao 
Published: March 2024

AbstractAeolian landforms provide valuable insights into the planetary surface environment and its evolutionary history. In this study, the formation and evolution of megaripples in the Qaidam Basin and their relationship with the development environment are analyzed. By quantifying the wind environment, morphology, grain‐size distribution, sedimentary structure, and luminescence age of megaripples, we propose for the first time that there are multiple megaripple evolution modes. Investigation revealed that three evolution modes were responsible for forming megaripples in different equilibrium states: transient, stable, and metastable. Well‐sorted coarse sand grains accumulate on ridges and overlay poorly sorted fine sand grains to form transient megaripples. Stable megaripples have alternating sedimentary bedding of coarse and fine sand grains. Metastable megaripples have a secondary ripple formation on the surface. Throughout their formation, coarse and fine sand grains undergo regrouping. The response of coarse grains to the change in wind speed lags behind that of fine grains. This process controls the erosion and accumulation of megaripples and affects their size and sedimentary structures. The evolution mode, scale, and sedimentary structure of megaripples are influenced by the grain‐size range under the same wind conditions. The luminescence ages of the coarse‐grained megaripple sediments are less than 700 years. This study provides a fresh perspective on the coexistence of various sand ripples and transverse aeolian ridges found on Mars.
Large Wood Transport and Accumulation Near the Separation Zone of a Channel Confluence
Authors: Yuan Saiyu , Zheng Yuchen , Tang Hongwu , Chen Yihong , Xu Lei , Whittaker Colin , Gualtieri Carlo 
Published: March 2024

AbstractFallen trees enter the adjacent stream and are carried away downstream by the current. As the stream joins another one, the complex hydrodynamics near their confluence make the movement of wood hard to predict. These woods may accumulate near the confluence resulting in backwater and subsequent potential flooding. A laboratory study was conducted to investigate the movement and accumulation behavior of individual pieces of wood near the confluence. The characteristics of wood (i.e., the length, diameter, and density) and the hydraulic conditions (i.e., the discharge ratio and the release distance) were varied in this investigation. It was found that the wooden pieces released from the tributary got occasionally trapped in the flow separation zone of the confluence, whereupon they were mainly trapped by a clockwise vortex and continued to stay driven by a reverse cluster of currents within this zone. The accumulation probability of wood was mainly related to its length, the discharge ratio and the release distance. The effect of wood diameter and density within the tested parameters was negligible. The probability increased with an increase in the discharge ratio as well as a decrease in the release distance. The longer pieces had a higher probability of being trapped, whereas for those exceeding some critical value, the probability was nearly the same, or dropped sharply. A generalized model for wood accumulation near the confluence was developed for practical application. These findings carry significant implications for river management, particularly in preventing the risk of flooding caused by wood blockage.
Comparing body composition between the sweet-liking phenotypes: experimental data, systematic review and individual participant data meta-analysis
Authors: Armitage Rhiannon Mae , Iatridi Vasiliki , Sladekova Martina , Yeomans Martin Richard 
Published: March 2024

Abstract
Background
Legislation aimed at reducing sugar intake assumes that sweet-liking drives overconsumption. However, evidence that a greater liking for sweet taste is associated with unhealthier body size is mixed and complicated by relatively small samples, an overreliance on body mass index (BMI) and lack of classification using sweet-liking phenotypes.

Methods
We first examined body size data in two larger samples with sweet-liking phenotyping: extreme sweet-likers, moderate sweet-likers and sweet-dislikers. Adults (18-34yrs), attended a two-session lab-based experiment involving phenotyping for sweet-liking status and a bioelectrical impedance body composition measurement (Experiment One: N = 200; Experiment Two: N = 314). Secondly, we conducted an individual participant data (IPD) meta-analysis: systematic searches across four databases identified 5736 potential articles. Of these, 53 papers met our search criteria: a taste assessment that measured liking using sucrose (>13.7% w/v), which allowed sweet-liking phenotyping and included either BMI, body fat percentage (BF%), fat-free mass (FFM) or waist-circumference.

Results
A significant effect of sweet-liking phenotype on FFM was found in both Experiment One and Two, with extreme sweet-likers having significantly higher FFM than sweet-dislikers. In Experiment One, sweet-dislikers had a significantly higher BF% than extreme sweet-likers and moderate sweet-likers. However, as these data are from one research group in a young, predominantly westernised population, and the results did not perfectly replicate, we conducted the IPD meta-analyses to further clarify the findings. Robust one-stage IPD meta-analyses of 15 studies controlling for sex revealed no significant differences in BF% (n = 1836) or waist-circumference (n = 706). For BMI (n = 2368), moderate sweet-likers had slightly lower BMI than extreme sweet-likers, who had the highest overall BMI. Most interestingly, for FFM (n = 768), moderate sweet-likers and sweet-dislikers showed significantly lower FFM than extreme sweet-likers.

Conclusion
The higher BMI often seen in sweet-likers may be due to a larger FFM and questions the simple model where sweet liking alone is a risk factor for obesity.
High-power, electrically-driven continuous-wave 1.55-μm Si-based multi-quantum well lasers with a wide operating temperature range grown on wafer-scale InP-on-Si (100) heterogeneous substrate
Authors: Sun Jialiang , Lin Jiajie , Zhou Min , Zhang Jianjun , Liu Huiyun , You Tiangui , Ou Xin 
Published: March 2024

AbstractA reliable, efficient and electrically-pumped Si-based laser is considered as the main challenge to achieve the integration of all key building blocks with silicon photonics. Despite the impressive advances that have been made in developing 1.3-μm Si-based quantum dot (QD) lasers, extending the wavelength window to the widely used 1.55-μm telecommunication region remains difficult. In this study, we develop a novel photonic integration method of epitaxial growth of III-V on a wafer-scale InP-on-Si (100) (InPOS) heterogeneous substrate fabricated by the ion-cutting technique to realize integrated lasers on Si substrate. This ion-cutting plus epitaxial growth approach decouples the correlated root causes of many detrimental dislocations during heteroepitaxial growth, namely lattice and domain mismatches. Using this approach, we achieved state-of-the-art performance of the electrically-pumped, continuous-wave (CW) 1.55-µm Si-based laser with a room-temperature threshold current density of 0.65 kA/cm−2, and output power exceeding 155 mW per facet without facet coating in CW mode. CW lasing at 120 °C and pulsed lasing at over 130 °C were achieved. This generic approach is also applied to other material systems to provide better performance and more functionalities for photonics and microelectronics.
Laser-upgraded coal tar for smart pavements in road and bridge monitoring applications
Authors: Huang Jincai , Zhang Man , He Haoyun , Li Qingang , Zhao Yixin , Tan Qiulin , Zang Xining 
Published: March 2024

AbstractThe implementation of an intelligent road network system requires many sensors for acquiring data from roads, bridges, and vehicles, thereby enabling comprehensive monitoring and regulation of road networks. Given this large number of required sensors, the sensors must be cost-effective, dependable, and environmentally friendly. Here, we show a laser upgrading strategy for coal tar, a low-value byproduct of coal distillation, to manufacture flexible strain-gauge sensors with maximum gauge factors of 15.20 and 254.17 for tension and compression respectively. Furthermore, we completely designed the supporting processes of sensor placement, data acquisition, processing, wireless communication, and information decoding to demonstrate the application of our sensors in traffic and bridge vibration monitoring. Our novel strategy of using lasers to upgrade coal tar for use as a sensor not only achieves the goal of turning waste into a resource but also provides an approach to satisfy large-scale application requirements for enabling intelligent road networks.
A first-in-class TIMM44 blocker inhibits bladder cancer cell growth
Authors: Zhang Lifeng , Shi Xiaokai , Zhang Lei , Mi Yuanyuan , Zuo Li , Gao Shenglin 
Published: March 2024

AbstractMitochondria play a multifaceted role in supporting bladder cancer progression. Translocase of inner mitochondrial membrane 44 (TIMM44) is essential for maintaining function and integrity of mitochondria. We here tested the potential effect of MB-10 (MitoBloCK-10), a first-in-class TIMM44 blocker, against bladder cancer cells. TIMM44 mRNA and protein expression is significantly elevated in both human bladder cancer tissues and cells. In both patient-derived primary bladder cancer cells and immortalized (T24) cell line, MB-10 exerted potent anti-cancer activity and inhibited cell viability, proliferation and motility. The TIMM44 blocker induced apoptosis and cell cycle arrest in bladder cancer cells, but failed to provoke cytotoxicity in primary bladder epithelial cells. MB-10 disrupted mitochondrial functions in bladder cancer cells, causing mitochondrial depolarization, oxidative stress and ATP reduction. Whereas exogenously-added ATP and the antioxidant N-Acetyl Cysteine mitigated MB-10-induced cytotoxicity of bladder cancer cells. Genetic depletion of TIMM44 through CRISPR-Cas9 method also induced robust anti-bladder cancer cell activity and MB-10 had no effect in TIMM44-depleted cancer cells. Contrarily, ectopic overexpression of TIMM44 using a lentiviral construct augmented proliferation and motility of primary bladder cancer cells. TIMM44 is important for Akt-mammalian target of rapamycin (mTOR) activation. In primary bladder cancer cells, Akt-S6K1 phosphorylation was decreased by MB-10 treatment or TIMM44 depletion, but enhanced after ectopic TIMM44 overexpression. In vivo, intraperitoneal injection of MB-10 impeded bladder cancer xenograft growth in nude mice. Oxidative stress, ATP reduction, Akt-S6K1 inhibition and apoptosis were detected in MB-10-treated xenograft tissues. Moreover, genetic depletion of TIMM44 also arrested bladder cancer xenograft growth in nude mice, leading to oxidative stress, ATP reduction and Akt-S6K1 inhibition in xenograft tissues. Together, targeting overexpressed TIMM44 by MB-10 significantly inhibits bladder cancer cell growth in vitro and in vivo.
USP22 regulates APL differentiation via PML-RARα stabilization and IFN repression
Authors: Kowald Lisa , Roedig Jens , Karlowitz Rebekka , Wagner Kristina , Smith Sonja , Juretschke Thomas , Beli Petra , Müller Stefan , van Wijk Sjoerd J. L. 
Published: March 2024

AbstractUbiquitin-specific peptidase 22 (USP22) is a deubiquitinating enzyme (DUB) that underlies tumorigenicity, proliferation, cell death and differentiation through deubiquitination of histone and non-histone targets. Ubiquitination determines stability, localization and functions of cell fate proteins and controls cell-protective signaling pathways to surveil cell cycle progression. In a variety of carcinomas, lymphomas and leukemias, ubiquitination regulates the tumor-suppressive functions of the promyelocytic leukemia protein (PML), but PML-specific DUBs, DUB-controlled PML ubiquitin sites and the functional consequences of PML (de)ubiquitination remain unclear. Here, we identify USP22 as regulator of PML and the oncogenic acute promyelocytic leukemia (APL) fusion PML-RARα protein stability and identify a destabilizing role of PML residue K394. Additionally, loss of USP22 upregulates interferon (IFN) and IFN-stimulated gene (ISG) expression in APL and induces PML-RARα stabilization and a potentiation of the cell-autonomous sensitivity towards all-trans retinoic acid (ATRA)-mediated differentiation. Our findings imply USP22-dependent surveillance of PML-RARα stability and IFN signaling as important regulator of APL pathogenesis, with implications for viral mimicry, differentiation and cell fate regulation in other leukemia subtypes.
Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage
Authors: Liu Jingtao , Tan Xiaomei , Li Li , Cao Liying , Zhou Yan , Li He , Peng Ting 
Published: March 2024

AbstractNucleolar protein 12 (NOL12), one of the nucleolar proteins which are primarily expressed in the nucleolus and play key roles in RNA metabolism, cell proliferation, cell cycle, and cell survival, is widely expressed in various species and multiple organs. Although it has been reported that the mRNA of Drosophila NOL12 homolog viriato is expressed in the eyes of Drosophila, the protein expression of NOL12 in mammalian eyes remains to be elucidated. In this study, we showed through immunohistochemistry that NOL12 was present in the rat retina, with predominant distribution in the cytoplasm of the retinal neuronal cells. In the human retinoblastoma cell line WERI-Rb1, we found that altering NOL12 expression led to a change in WERI-Rb1 cell viability. Knocking down NOL12 expression decreased cell viability. In contrast, overexpressing NOL12 increased cell viability. Furthermore, increasing NOL12 expression inhibited ultraviolet (UV)-induced apoptosis. These findings demonstrated that NOL12 may play an important protective role in retinal cells. In the WERI-Rb1 cells exposed to UV irradiation, we detected that NOL12 was degraded, but this degradation could be attenuated by a pan-Caspase inhibitor. Notably, the inhibitory effect of NOL12 against UV-induced apoptosis could be restrained by increasing the expression of ATR serine/threonine kinase (ATR), a kinase that, when activated by severe DNA damage, can result in apoptosis. We also found that upregulating NOL12 inhibited the activation of ATR caused by UV irradiation. Additionally, inhibiting ATR activity reduced apoptosis resulting from both silencing NOL12 expression and UV exposure. Thus, NOL12 may protect against UV irradiation-induced retinal damage by inhibiting ATR activity.
A second hotspot for pathogenic exon-skipping variants in CDC45
Authors: Schoch Kelly , Ruegg Mischa S. G. , Fellows Bridget J. , Cao Joseph , Uhrig Sabine , Einsele-Scholz Stephanie , Biskup Saskia , Hawarden Samuel R. A. , Salpietro Vincenzo , Capra Valeria ,   Undiagnosed Diseases Network, Brown Chris M. , Accogli Andrea , Shashi Vandana , Bicknell Louise S. 
Published: March 2024

AbstractBiallelic pathogenic variants in CDC45 are associated with Meier-Gorlin syndrome with craniosynostosis (MGORS type 7), which also includes short stature and absent/hypoplastic patellae. Identified variants act through a hypomorphic loss of function mechanism, to reduce CDC45 activity and impact DNA replication initiation. In addition to missense and premature termination variants, several pathogenic synonymous variants have been identified, most of which cause increased exon skipping of exon 4, which encodes an essential part of the RecJ-orthologue’s DHH domain. Here we have identified a second cohort of families segregating CDC45 variants, where patients have craniosynostosis and a reduction in height, alongside common facial dysmorphisms, including thin eyebrows, consistent with MGORS7. Skipping of exon 15 is a consequence of two different variants, including a shared synonymous variant that is enriched in individuals of East Asian ancestry, while other variants in trans are predicted to alter key intramolecular interactions in α/β domain II, or cause retention of an intron within the 3ʹUTR. Our cohort and functional data confirm exon skipping is a relatively common pathogenic mechanism in CDC45, and highlights the need for alternative splicing events, such as exon skipping, to be especially considered for variants initially predicted to be less likely to cause the phenotype, particularly synonymous variants.
Understanding the infection severity and epidemiological characteristics of mpox in the UK
Authors: Ward Thomas , Overton Christopher E. , Paton Robert S. , Christie Rachel , Cumming Fergus , Fyles Martyn 
Published: March 2024

AbstractIn May 2022, individuals infected with the monkeypox virus were detected in the UK without clear travel links to endemic areas. Understanding the clinical characteristics and infection severity of mpox is necessary for effective public health policy. The study period of this paper, from the 1st June 2022 to 30th September 2022, included 3,375 individuals that tested positive for the monkeypox virus. The posterior mean times from infection to hospital admission and length of hospital stay were 14.89 days (95% Credible Intervals (CrI): 13.60, 16.32) and 7.07 days (95% CrI: 6.07, 8.23), respectively. We estimated the modelled Infection Hospitalisation Risk to be 4.13% (95% CrI: 3.04, 5.02), compared to the overall sample Case Hospitalisation Risk (CHR) of 5.10% (95% CrI: 4.38, 5.86). The overall sample CHR was estimated to be 17.86% (95% CrI: 6.06, 33.11) for females and 4.99% (95% CrI: 4.27, 5.75) for males. A notable difference was observed between the CHRs that were estimated for each sex, which may be indicative of increased infection severity in females or a considerably lower infection ascertainment rate. It was estimated that 74.65% (95% CrI: 55.78, 86.85) of infections with the monkeypox virus in the UK were captured over the outbreak.
Mitochondrial metabolism sustains CD8+ T cell migration for an efficient infiltration into solid tumors
Authors: Simula Luca , Fumagalli Mattia , Vimeux Lene , Rajnpreht Irena , Icard Philippe , Birsen Gary , An Dongjie , Pendino Frédéric , Rouault Adrien , Bercovici Nadège , Damotte Diane , Lupo-Mansuet Audrey , Alifano Marco , Alves-Guerra Marie-Clotilde , Donnadieu Emmanuel 
Published: March 2024

AbstractThe ability of CD8+ T cells to infiltrate solid tumors and reach cancer cells is associated with improved patient survival and responses to immunotherapy. Thus, identifying the factors controlling T cell migration in tumors is critical, so that strategies to intervene on these targets can be developed. Although interstitial motility is a highly energy-demanding process, the metabolic requirements of CD8+ T cells migrating in a 3D environment remain unclear. Here, we demonstrate that the tricarboxylic acid (TCA) cycle is the main metabolic pathway sustaining human CD8+ T cell motility in 3D collagen gels and tumor slices while glycolysis plays a more minor role. Using pharmacological and genetic approaches, we report that CD8+ T cell migration depends on the mitochondrial oxidation of glucose and glutamine, but not fatty acids, and both ATP and ROS produced by mitochondria are required for T cells to migrate. Pharmacological interventions to increase mitochondrial activity improve CD8+ T cell intratumoral migration and CAR T cell recruitment into tumor islets leading to better control of tumor growth in human xenograft models. Our study highlights the rationale of targeting mitochondrial metabolism to enhance the migration and antitumor efficacy of CAR T cells in treating solid tumors.
Local orchestration of distributed functional patterns supporting loss and restoration of consciousness in the primate brain
Authors: Luppi Andrea I. , Uhrig Lynn , Tasserie Jordy , Signorelli Camilo M. , Stamatakis Emmanuel A. , Destexhe Alain , Jarraya Bechir , Cofre Rodrigo 
Published: March 2024

AbstractA central challenge of neuroscience is to elucidate how brain function supports consciousness. Here, we combine the specificity of focal deep brain stimulation with fMRI coverage of the entire cortex, in awake and anaesthetised non-human primates. During propofol, sevoflurane, or ketamine anaesthesia, and subsequent restoration of responsiveness by electrical stimulation of the central thalamus, we investigate how loss of consciousness impacts distributed patterns of structure-function organisation across scales. We report that distributed brain activity under anaesthesia is increasingly constrained by brain structure across scales, coinciding with anaesthetic-induced collapse of multiple dimensions of hierarchical cortical organisation. These distributed signatures are observed across different anaesthetics, and they are reversed by electrical stimulation of the central thalamus, coinciding with recovery of behavioural markers of arousal. No such effects were observed upon stimulating the ventral lateral thalamus, demonstrating specificity. Overall, we identify consistent distributed signatures of consciousness that are orchestrated by specific thalamic nuclei.