bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Analysis of Parasite Load on Recently Established Invasive Pumpkinseed Lepomis gibbosus (Actinopterygii: Centrarchidae) in Europe
Authors: Dudliv Ivanna , Kvach Yuriy , Tkachenko Maria Yu. , Nazaruk Kateryna , Ondračková Markéta 
Published: March 2024

Abstract
Purpose
The aim of this study was the comparative analysis of the parasite communities of new populations of invasive pumpkinseed (Lepomis gibbosus) in western Ukraine with pumpkinseed from Czechia, where populations have rapidly expanded over the last two decades.

Methods
Sampling took place at three localities in the western part of Ukraine (i.e. Dobrotvir Reservoir (Vistula basin), Burshtyn Reservoir (Dniester basin), Mynai Pond (Danube basin)) and four in Czechia (i.e. Oxbow D2, Heršpický Pond (Danube basin), and Kolín oxbow and Římov Reservoir (Elbe basin).

Results
In total, 11 parasite taxa were recorded in Ukraine and 17 in Czechia. Four species were co-introduced from North America with their host, i.e. the myxosporean Myxobolus dechtiari, the monogeneans Onchocleidus dispar and Onchocleidus similis, and metacercariae of a trematode Posthodiplostomum centrarchi. High dominance indices were related to a high abundance of co-introduced parasites, i.e. O. similis in Mynai pond and P. centrarchi in Dobrotvir Reservoir. Overall abundance of acquired parasites was generally low.

Conclusion
This study shows that parasite communities in recently established pumpkinseed populations in the western part of Ukraine and Czechia are less diverse than those established in Europe for decades. The generally low parasite load in these new populations may play an important role in their ability to successfully establish and create strong populations by providing a competitive advantage over local species.
Trace Minerals in Laying Hen Diets and Their Effects on Egg Quality
Authors: Elnesr Shaaban S. , Mahmoud Bothaina Y. , da Silva Pires Paula Gabriela , Moraes Priscila , Elwan Hamada A. M. , El-Shall Nahed Ahmed , El-Kholy Mohamed S. , Alagawany Mahmoud 
Published: March 2024

AbstractWith the advancement in the egg industry sector, egg quality has assumed great significance in certain countries. Enhancements in the nutritional value of eggs may have direct affirmative consequences for daily nutrient intake and therefore for human health. Thus, affirmative improvement in egg quality boosts consumer preferences for eggs. Also, the improvement in eggshell quality can avoid the disposal of broken eggs and consequently economic losses. Therefore, poultry nutrition and mineral supplements have a significant impact on egg quality. Minerals are crucial in poultry feed for a number of biological processes, including catalytic, physiologic, and structural processes. For instance, they contribute to the biological processes necessary for forming and developing eggshells. To produce high-quality eggs for sale, diets must therefore contain the right amount of minerals. This review aims to highlight the role of both organic and inorganic minerals in improving egg quality, in addition to reviewing the interactions of mineral supplements with intestinal microbiota and subsequent effects on the egg quality.
Adolescent gaming and parent–child emotional closeness: bivariate relationships in a longitudinal perspective
Authors: Boson Karin , Gurdal Sevtap , Claesdotter-Knutsson Emma , Kapetanovic Sabina 
Published: March 2024

AbstractThe aim of this study was to add knowledge of the longitudinal associations between gaming and emotional closeness between parents and their children. We hypothesized that parent–child emotional closeness was linked to less gaming activity over time and that more gaming activity was linked to less parent–child emotional closeness over time. We also tested the moderating effect of child gender on these anticipated links. This study involved a sample of Swedish adolescents, spanning the developmental years from age 12.5 to 17, and included data from two time points (T1; year 2013 and T2; years 2017/2018) with N = 782 participants (T1 Mage = 12.10, SD = 0.40; 49.6% girls). Utilizing a series of Cross-Lagged Panel Models, we found that emotional closeness to both mother and father predicted less time spent on gaming over time. More time spent on gaming predicted less emotional closeness to mother over time. Additionally, gaming activity among girls was specifically related to less emotional closeness to their father over time. Strengthening parent–child relationships and emotional bonds may be crucial in safeguarding adolescents from developing habits of excessive gaming that could potentially pose problems for their psychosocial development.
A Test for Global Attractivity of Linear Dynamic Equations with Delay
Authors: Alsharif Nour H. M. , Karpuz Basak 
Published: March 2024

AbstractIn this paper, we consider the delay dynamic equation Based on Lyapunov’s method, we study global attractivity of the trivial solution of ($$*$$

∗

). Our new result generalizes some well-known results in the theory of difference and differential equations to dynamic equations of the form ($$*$$

∗

). We also present some examples on nonstandard time scales to illustrate the importance of the new result.
Molecular Dynamic Study on the Structure and Thermal Stability of Mutant Pediocin PA-1 Peptides Engineered with Cysteine Substitutions
Authors: SEVİM Büşra , GÜNEŞ ALTUNTAŞ Evrim 
Published: March 2024

AbstractPediocin and analogous bacteriocins, valued for thermal stability, serve as versatile antimicrobials in the food sector. Improving their resilience at high temperatures and deriving derivatives not only benefit food production but also offer broad-spectrum antimicrobial potential in pharmaceuticals, spanning treatments for peptic ulcers, women’s health, and novel anticancer agents. The study aims to create mutant peptides capable of establishing a third disulfide bond or enhanced through cysteine substitutions. This involves introducing additional Cys residues into the inherent structure of pediocin PA-1 to facilitate disulfide bond formation. Five mutants (Mut 1–5) were systematically generated with double Cys substitutions and assessed for thermal stability through MD simulations across temperatures (298–394 K). The most robust mutants (Mut 1, Mut 4–5) underwent extended analysis via MD simulations, comparing their structural stability, secondary structure, and surface accessibility to the reference Pediocin PA-1 molecule. This comprehensive assessment aims to understand how Cys substitutions influence disulfide bonds and the overall thermal stability of the mutant peptides. In silico analysis indicated that Mut 1 and Mut 5, along with the reference structure, lose their helical structure and one natural disulfide bond at high temperatures, and may impacting antimicrobial activity. Conversely, Mut 4 retained its helical structure and exhibited thermal stability similar to Pediocin PA-1. Pending further experimental validation, this study implies Mut 4 may have high stability and exceptional resistance to high temperatures, potentially serving as an effective antimicrobial alternative.
The urgency for a change in genetics healthcare provision: views from Portuguese medical geneticists
Authors: Costa Catarina , Guimarães Lídia , Baião Ruxanda Lungu , Lemos Marina Serra de , Azevedo Luís Filipe , Paneque Milena 
Published: March 2024

AbstractIn the last decades, genetics has experienced significant technological advancements worldwide. However, in Portugal, serious limitations persist, compromising the functioning of healthcare in medical genetics. This study aimed to promote sharing and discussion among genetic medical professionals, to outline concrete actions to address gaps in clinical practice. Three focus groups were conducted with 19 specialists in medical genetics. The data were analyzed using the thematic analysis method to extract the main themes from the discussions. From the analysis, four conceptual themes emerged: (i) framing Portuguese genetic services in light of the European context; (ii) improvement of medical genetics education and population literacy; (iii) transforming of medical genetics services; and (iv) operationalizing the change. The results demonstrated that increasing training resources and strengthening multiprofessional teams by hiring more genetic professionals, such as clinical geneticists, molecular geneticists, and other genetic specialists, is crucial to enhancing the responsiveness of genetic services. Integrating medical genetics into all specialties and primary care, as well as updating the national network of medical genetics, are critical points for increasing equity and enabling healthcare to be provided more fairly. Including other medical genetics professionals such as genetic counsellors, nurses and psychologists also plays a significant role in providing comprehensive and quality care. This collaborative approach aims to provide effective genetic assistance and enhance the adequacy of genetic healthcare. The findings are compiled as recommendations to support the profession moving forward that can be applied to other healthcare contexts worldwide.
PLGA-PEI nanoparticle covered with poly(I:C) for personalised cancer immunotherapy
Authors: Gonzalez-Melero Lorena , Santos-Vizcaino Edorta , Varela-Calvino Ruben , Gomez-Tourino Iria , Asumendi Aintzane , Boyano Maria Dolores , Igartua Manoli , Hernandez Rosa Maria 
Published: March 2024

AbstractMelanoma is the main cause of death among skin cancers and its incidence worldwide has been experiencing an appalling increase. However, traditional treatments lack effectiveness in advanced or metastatic patients. Immunotherapy, meanwhile, has been shown to be an effective treatment option, but the rate of cancers responding remains far from ideal. Here we have developed a personalized neoantigen peptide-based cancer vaccine by encapsulating patient derived melanoma neoantigens in polyethylenimine (PEI)-functionalised poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and coating them with polyinosinic:polycytidylic acid (poly(I:C)). We found that PLGA NPs can be effectively modified to be coated with the immunoadjuvant poly(I:C), as well as to encapsulate neoantigens. In addition, we found that both dendritic cells (DCs) and lymphocytes were effectively stimulated. Moreover, the developed NP was found to have a better immune activation profile than NP without poly(I:C) or without antigen. Our results demonstrate that the developed vaccine has a high capacity to activate the immune system, efficiently maturing DCs to present the antigen of choice and promoting the activity of lymphocytes to exert their cytotoxic function. Therefore, the immune response generated is optimal and specific for the elimination of melanoma tumour cells.
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The Interplay Between Dietary Choline and Cardiometabolic Disorders: A Review of Current Evidence
Authors: Vallianou Natalia G. , Kounatidis Dimitris , Psallida Sotiria , Panagopoulos Fotis , Stratigou Theodora , Geladari Eleni , Karampela Irene , Tsilingiris Dimitrios , Dalamaga Maria 
Published: March 2024

Abstract
Purpose of Review
Choline is an essential nutrient for human health and cellular homeostasis as it is necessary for the synthesis of lipid cell membranes, lipoproteins, and the synthesis of the neurotransmitter acetylcholine. The aim of this review is to analyze the beneficial effects of choline and its significance in cellular metabolism and various inflammatory pathways, such as the inflammasome. We will discuss the significance of dietary choline in cardiometabolic disorders, such as non-alcoholic fatty liver disease (NAFLD), cardiovascular disease (CVD), and chronic kidney disease (CKD) as well as in cognitive function and associated neuropsychiatric disorders.

Recent Findings
Choline deficiency has been related to the development of NAFLD and cognitive disability in the offspring as well as in adulthood. In sharp contrast, excess dietary intake of choline mediated via the increased production of trimethylamine by the gut microbiota and increased trimethylamine-N-oxide (TMAO) levels has been related to atherosclerosis in most studies. In this context, CVD and CKD through the accumulation of TMAO, p-Cresyl-sulfate (pCS), and indoxyl-sulfate (IS) in serum may be the result of the interplay between excess dietary choline, the increased production of TMAO by the gut microbiota, and the resulting activation of inflammatory responses and fibrosis.

Summary
A balanced diet, with no excess nor any deficiency in dietary choline, is of outmost importance regarding the prevention of cardiometabolic disorders as well as cognitive function. Large-scale studies with the use of next-generation probiotics, especially Akkermansia muciniphila and Faecalibacterium prausnitzii, should further examine their therapeutic potential in this context.
Prioritizing Strategies for Building the Resilience of Public Health Systems to Disasters Across Multiple Communities and Countries
Authors: Ryan Benjamin J. , Kako Mayumi , Garner Shelby , Fink Rok , Tayfur Ismail , Abrahams Jonathan , Bhatia Sanjaya , Campelo Adriana , Fendt Matthew , Fontenot Alicia , Garcia Nahuel Arenas , Hatch Tim , Kayano Ryoma , Malrey-Horne LaShonda , MacDermot Makiko , Rahman Md Moshiur , Noel Chaverle , Nomura Shuhei , Novak Jeremy P. , Opazo Maria , Oliver Kendell , Peters Luciana , Rahman Sohel , Şimşek Perihan , Stricklin Andrew , Swienton Raymond , Brooks Bryan W. 
Published: March 2024

AbstractThe COVID-19 pandemic highlighted the urgent need to strengthen public health systems. In response, the United Nations Disaster Risk Reduction (UNDRR) Public Health System Resilience Scorecard (Scorecard) was applied in workshops across multiple countries. The aim of our research was to explore the workshop findings to develop priority strategies for strengthening public health system resilience. We conducted a workshop from 14 to 16 March 2023, at the UNDRR Global Education and Training Institute in Incheon, Republic of Korea. A sequential modified Delphi method was utilized to develop a set of prioritized resilience strategies. These were drawn from 70 strategies identified from 13 distinct workshops in eight countries. After two surveys, 23 strategies were finalized. Ten received ratings of “High” or “Very High” from 89% of participants. These related to the inclusion of public health risks in emergency plans, integrating multidisciplinary teams into public health, enabling local transport mechanisms, and improving the ability to manage an influx of patients. The Scorecard provides an adaptable framework to identify and prioritize strategies for strengthening public health system resilience. By leveraging this methodology, our study demonstrated how resilience strategies could inform disaster risk reduction funding, policies, and actions.
Preadmission course and management of severe pediatric group A streptococcal infections during the 2022–2023 outbreak: a single-center experience
Authors: Schöbi Nina , Duppenthaler Andrea , Horn Matthias , Bartenstein Andreas , Keitel Kristina , Kopp Matthias V. , Agyeman Philipp , Aebi Christoph 
Published: March 2024

Abstract
Purpose
The massive increase of infections with Group A Streptococcus (GAS) in 2022–2023 coincided in Switzerland with a change of the recommendations for the management of GAS pharyngitis. Therefore, the objective of the present study was to investigate whether the clinical manifestations and management before hospitalization for GAS infection differed in 2022–2023 compared with 2013–2022.

Methods
Retrospective study of GAS infections requiring hospitalization in patients below 16 years. Preadmission illness (modified McIsaac score), oral antibiotic use, and outcome in 2022–2023 were compared with 2013–2022. Time series were compared with surveillance data for respiratory viruses.

Results
In 2022–2023, the median modified McIsaac score was lower (2 [IQR 2–3] vs. 3 [IQR 2–4], p =  < 0.0001) and the duration of preadmission illness was longer (4 days [3–7] vs. 3 [2–6], p = 0.004) than in 2013–2022. In both periods, withholding of preadmission oral antibiotics despite a modified McIsaac score ≥ 3 (12% vs. 18%, n.s.) or ≥ 4 (2.4% vs. 10.0%, p = 0.027) was rare. Respiratory disease, skeletal/muscle infection, and invasive GAS disease were significantly more frequent in 2022–2023, but there were no differences in clinical outcome. The time course of GAS cases in 2022–2023 coincided with the activity of influenza A/B.

Conclusion
We found no evidence supporting the hypothesis that the 2022–2023 GAS outbreak was associated with a change in preadmission management possibly induced by the new recommendation for GAS pharyngitis. However, clinical manifestations before admission and comparative examination of time-series strongly suggest that viral co-circulation played an important role in this outbreak.
Working seagrasses: emerging coastal ethics in the Mexican Caribbean
Authors: Otto Laura 
Published: March 2024

AbstractThe article explores the emergence of coastal ethics in the Anthropocene, focusing on the Riviera Maya in Mexico. In response to escalating challenges such as coastal degradation and Sargassum impact, the study shifts the focus from blame to the practices of marine biologists engaged in repairing ecosystems, particularly seagrasses. The concept of “working seagrasses” is introduced, emphasizing the functional, performative, and manipulative aspects of human-seagrass interactions. Through ethnographic fieldwork, the author observes a departure from blame-based approaches prevalent in the field towards marine biologists, who actively work to repair seagrass beds. Three empirical examples illustrate different dimensions of working seagrasses, shedding light on scientists’ curated interactions, ecological restoration practices, and the role of species recognition in coastal ethics. The study explores multi-species entanglements along Mexican coasts, emphasizing collaborative efforts between humans and non-humans. By addressing how marine biologists respond to coastal degradation and involving non-human actors, the study contributes to understanding evolving coastal ethics in the Anthropocene.
Developing Flexible Risk Management Systems for Resilience in a Post-pandemic World: Can Lessons from a Makerspace Case Study Support Pacific Island Communities?
Authors: Tootell Adrian , Treadwell Leanne , Schulz Rebekah , Margetts Belinda , Grozdanov Jessica , Spinks Geoff 
Published: March 2024

AbstractPacific Island communities are facing disruptions to supply chains from natural disasters and a changing global environment, which have become more acute following the COVID-19 pandemic. Further, it has been demonstrated how flexible systems can enhance resilience in low-resource environments, such as adapting to changing consumer needs and minimizing supply chain disruptions. This paper considers how the development of a flexible system for conducting a risk assessment on a product that was developed and manufactured in a Makerspace environment would have application in Pacific Island communities to improve resilience. Using a participative action research (PAR) approach, a traditional product risk assessment is refined through iterative PAR cycles to reconceptualize it into a structured simplified risk process. The resulting product development risk assessment process (PDRAP) demonstrates that it is possible to adapt a detailed systematic risk assessment process, such as hazard and operability analysis (HAZOP), to be more suitable and effective for low-resource situations requiring flexible solutions. The improved process provides greater system flexibility to empower people to develop products which may improve their resilience in an ever changing and complex world. The PDRAP process can improve product design and adaptability which assists safeguarding supply chains from system wide disruptions. With the emergence of Makerspaces in developing countries for supply chain recovery from natural disasters and a changing national strategy, the PDRAP provides communities with a low-resource approach for risk assessment to ensure the safe use of products fabricated using emerging low-volume, rapid prototyping, and manufacturing technology.
Educational interventions in pharmacovigilance to improve the knowledge, attitude and the report of adverse drug reactions in healthcare professionals: Systematic Review and Meta-analysis
Authors: Cervantes-Arellano Mónica J. , Castelán-Martínez Osvaldo D. , Marín-Campos Yolanda , Chávez-Pacheco Juan L. , Morales-Ríos Olga , Ubaldo-Reyes Laura M. 
Published: March 2024

Abstract
Objectives
Underreporting of adverse drug reactions (ADRs) limits and delays the detection of signs. The aim of this systematic review with meta-analyses was to synthesize the evidence of educational interventions (EIs) efficacy in health professionals to increase ADR reporting, attitudes, and knowledge of pharmacovigilance.

Evidence acquisition
A systematic literature review was carried out to identify randomized clinical trials evaluating the efficacy of EI in pharmacovigilance in health professionals to improve ADR reports, knowledge, and attitude toward pharmacovigilance. ADR reports were pooled by calculating Odds Ratio (OR) with a 95% confidence interval (95%CI), while pharmacovigilance knowledge and attitude were pooled by calculating a mean difference (MD) with 95%CI. In addition, the subanalysis was performed by EI type. Meta-analysis was performed with RevMan 5.4 software. PROSPERO registry CRD42021254270.

Results
Eight hundred seventy-five articles were identified as potentially relevant, and 11 were included in the systematic review. Metanalysis showed that EI increased ADR reporting in comparison with control group (OR = 4.74, [95%CI, 2.46 to 9.12], I2 = 93%, 5 studies). In subgroup analysis, the workshops (OR = 6.26, [95%CI, 4.03 to 9.73], I2 = 57%, 3 studies) increased ADR reporting more than telephone-based interventions (OR = 2.59, [95%CI, 0.77 to 8.73], I2 = 29%, 2 studies) or combined interventions (OR = 5.14, [95%CI, 0.97 to 27.26], I2 = 93%, 3 studies). No difference was observed in pharmacovigilance knowledge. However, the subanalysis revealed that workshops increase pharmacovigilance knowledge (SMD = 1.85 [95%CI, 1.44 to 2.27], 1 study). Only one study evaluated ADR reporting attitude among participants and showed a positive effect after the intervention.

Conclusion
EI improves ADR reports and increases pharmacovigilance knowledge. Workshops are the most effective EI to increase ADR reporting.

Graphical abstract
Incorporation of multilayered double hydroxides/sepiolite augments proton conductivity performance in low sulfonated polyether sulfone octyl sulfonamide
Authors: Charradi khaled , Mabrouk Walid , Kacem Imen Ben , Bellakhal Nizar , Al-Ghamdi Youssef O. , Marzouki Riadh , Keshk Sherif M. A. S. 
Published: March 2024

AbstractLow-sulfonation-level polyether sulfone octyl sulfonamide (LSPSO) was blended with a layered double hydroxides (LDHs, Mg2AlCl)/sepiolite nanostructure clay as a filler to create an electrolyte membrane for fuel cell applications. Comprehensive characterization of the composite membranes was conducted, encompassing Fourier-transform infrared spectroscopy, X-ray diffraction, mechanical stability assessment, thermal gravimetric analysis, ion exchange capability, swelling characteristics, water uptake performance, and electrochemical impedance spectroscopy analysis. In comparison to the pristine LSPSO membrane, the presence of LDHs/sepiolite nanoarchitecture material within LSPSO exhibited superior water retention and proton conductivity values, especially at elevated temperatures. The proton conductivity of the composite membranes reached approximately 250 mS/cm, while the unmodified LSPSO membrane only achieved 35 mS/cm at 100 °C. Moreover, LSPSO composite membranes demonstrated enhanced chemical and thermal stability along with higher proton conductivity when compared to pristine LSPSO membranes. These findings highlight the potential of developing tailored LSPSO composite membranes to advance the prospects of commercial applications in proton exchange membrane fuel cells.