bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reusable, reversibly sealable parylene membranes for cell and protein patterning
Authors: Wright Dylan , Rajalingam Bimalraj , Karp Jeffrey M. , Selvarasah Selvapraba , Ling Yibo , Yeh Judy , Langer Robert , Dokmeci Mehmet R. , Khademhosseini Ali 
Published: August 2007

AbstractThe patterned deposition of cells and biomolecules on surfaces is a potentially useful tool for in vitro diagnostics, high‐throughput screening, and tissue engineering. Here, we describe an inexpensive and potentially widely applicable micropatterning technique that uses reversible sealing of microfabricated parylene‐C stencils on surfaces to enable surface patterning. Using these stencils it is possible to generate micropatterns and copatterns of proteins and cells, including NIH‐3T3 fibroblasts, hepatocytes and embryonic stem cells. After patterning, the stencils can be removed from the surface, plasma treated to remove adsorbed proteins, and reused. A variety of hydrophobic surfaces including PDMS, polystyrene and acrylated glass were patterned using this approach. Furthermore, we demonstrated the reusability and mechanical integrity of the parylene membrane for at least 10 consecutive patterning processes. These parylene‐C stencils are potentially scalable commercially and easily accessible for many biological and biomedical applications. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008
Biomechanical properties of carbodiimide crosslinked collagen: Influence of the formation of ester crosslinks
Authors: Everaerts Frank , Torrianni Mark , Hendriks Marc , Feijen Jan 
Published: August 2007

AbstractThere is a growing interest in the use of collagen matrices for tissue engineering. To prevent rapid degradation and to improve their mechanical properties, collagen matrices have been modified using different crosslinking agents. Among the different agents used, water soluble carbodiimides (such as N′‐(3‐dimethylaminopropyl)‐N‐ethylcarbodiimide, EDC) in combination with N‐hydroxysuccinimide (NHS) are attractive systems, because no additional chemical entities are incorporated in the matrix. EDC/NHS crosslinking leads to amide bond formation between activated carboxyl groups and amine groups. Recently, we proposed that in addition to amide bond formation, ester links are also formed between activated carboxyl groups and hydroxyl groups. This was based on observations we made after development of a new method to quantify concentrations of carboxyl groups of collagen materials before and after crosslinking. The current study is directed to the influence of ester bond crosslinks formed after crosslinking of collagen with EDC/NHS on its physical–chemical and biomechanical properties. Reconstituted dermal bovine collagen patches (RDBC) were used as model material and were crosslinked with EDC/NHS. In one RDBC group, collagen amine groups were blocked with propionaldehyde prior to crosslinking, while in the other group unprocessed RDBC was crosslinked without additional matrix modifications. It was shown that after activation of collagen carboxyl groups with EDC and NHS, amide crosslinks as well as ester crosslinks with collagen hydroxyl groups were formed. It was furthermore demonstrated that the ester crosslinks of EDC/NHS‐crosslinked RDBC could be removed by mild hydrolysis affording collagen matrices with improved mechanical properties. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Effect of Salvia miltiorrhiza extract on bone formation
Authors: Wong R. W. K. , Rabie A. B. M. 
Published: August 2007

AbstractSalvia miltiorrhiza (SM) is a Chinese medicine used for treatment of cardiac diseases through improving blood circulation. Our laboratory compared the amount of new bone formed in collagen matrix with SM extract to that formed in the collagen matrix alone. Eighteen bone defects, 5 mm × 10 mm were created in the parietal bone of nine New Zealand White rabbits. In the experimental group, six defects were grafted with collagen matrix with SM extract. In the control groups, six defects were grafted with collagen matrix alone (positive control) and six were left empty (negative control). Animals were killed on day 14 and the defects were dissected and prepared for histological assessment. Serial sections were cut across each defect. Quantitative analysis of new bone formation was made on 100 sections (50 sections for each group of SM and positive control) using image analysis. A total of 478% more new bone was present in defects grafted with SM extract in collagen matrix than those grafted with the collagen matrix alone. Very little new bone was formed in the passive control group. In conclusion, SM extract in collagen matrix has the effect of increasing new bone formation locally and can be used for bone grafting especially in cases with compromised vascular responses. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Vaccines to treat cancer‐an old approach whose time has arrived
Authors: Hellstrom Karl Erik , Hellstrom Ingegerd 
Published: August 2007

AbstractThere are extensive DNA changes in tumor cells and the genes of tumor cells continuously mutate at a high rate. While this can provide therapeutic targets, it makes it unlikely that an agent that is selective for a single target will work against all cells in a tumor. However, it may be possible to use tumor epitopes as sentinels to engage adaptive and innate immunological mechanisms and create a tumor destructive environment effective also against variant cells that have lost a given antigen or their ability to present it. We hypothesize that therapeutic tumor vaccines, in combination with the targeting, to tumors, of costimulatory molecules such as anti‐CD137scFv, or lymphokines such as GMCSF, will expand anti‐tumor responses for therapeutic benefit when used as an adjunct to surgery and chemotherapy. J. Cell. Biochem. 102: 291–300, 2007. © 2007 Wiley‐Liss, Inc.
TGF‐β signaling: A tale of two responses
Authors: Rahimi Rod A. , Leof Edward B. 
Published: August 2007

AbstractTransforming growth factor‐β (TGF‐β) regulates a wide variety of cellular processes including cell growth, apoptosis, differentiation, migration, and extracellular matrix production among others. The canonical signaling pathway induced by the TGF‐β receptor complex involves the phosphorylation of Smad proteins which upon activation accumulate in the nucleus and regulate transcription. Interestingly, the cellular response to TGF‐β can be extremely variable depending on the cell type and stimulation context. TGF‐β causes epithelial cells to undergo growth arrest and apoptosis, responses which are critical to suppressing carcinogenesis, whereas it can also induce epithelial‐mesenchymal transition and mediate fibroblast activation, responses implicated in promoting carcinogenesis and fibrotic diseases. However, TGF‐β induces all these responses via the same receptor complex and Smad proteins. To address this apparent paradox, during the last few years a number of additional signaling pathways have been identified which potentially regulate the different cellular responses to TGF‐β. The identification of these signaling pathways has shed light onto the mechanisms whereby Smad and non‐Smad pathways collaborate to induce a particular cellular phenotype. In this article, we review TGF‐β signaling in epithelial cells and fibroblasts with a focus on understanding the mechanisms of TGF‐β versatility. J. Cell. Biochem. 102: 593–608, 2007. © 2007 Wiley‐Liss, Inc.
A potent replicative delta‐24 adenoviral vector driven by the promoter of human papillomavirus 16 that is highly selective for associated neoplasms
Authors: Delgado‐Enciso Iván , Cervantes‐García Daniel , Martínez‐Dávila Irma A. , Ortiz‐López Rocío , Alemany‐Bonastre Ramón , Silva‐Platas Christian I. , Lugo‐Trampe Ángel , Barrera‐Saldaña Hugo A. , Galván‐Salazar Héctor R. , Coronel‐Tene Christian G. , Sánchez‐Santillán Carlos F. , Rojas‐Martínez Augusto 
Published: August 2007

AbstractBackgroundSeveral human epithelial neoplasms are associated with high‐risk strains of human papillomavirus (HPV) such as cervical, anorectal, and other carcinomas. For some tumor types the current therapeutic tools are only palliative. Conditionally replicative adenoviruses (CRAds) are promising antineoplastic agents, which also can trigger confined antitumor effects.MethodsWe constructed a series of CRAds driven by the upstream regulatory promoter region (URR) of an Asian‐American variant of HPV‐16, which contained different mutations at the E1A region (dl1015 and/or Δ24) and wild‐type. All vectors were tested in vitro for viral replication and cytotoxicity. Viral DNA replication and E1A expression were also assessed by quantitative PCR. Finally, we confirmed the antitumoral efficacy of this vector in injected and non‐injected xenotransplanted cervical tumors in a murine model for tumor regression and survival studies.ResultsA vector denominated Ad‐URR/E1AΔ24 displayed a potent cytopathic effect associated with high selectivity for HPV+ cell lines. We found that the oncolytic effect of this CRAd was comparable to Ad‐wt or Ad‐Δ24, but this efficacy was significantly attenuated in HPV− cell lines, an effect that was contributed by the URR promoter. Ad‐URR/E1AΔ24 was very effective to control tumor growth, in both, injected and non‐injected tumors generated with two different HPV+ cell lines.ConclusionsCRAd Ad‐URR/E1AΔ24 is a highly selective vector for HPV+ cell lines and tumors that preserves the oncolytic efficacy of Ad‐wt and Ad‐Δ24. Our preclinical data suggest that this vector may be useful and safe for the treatment of tumors induced by HPV, like cervical cancers. Copyright © 2007 John Wiley & Sons, Ltd.
Modelling bioclimate by means of Fourier analysis of NOAA‐AVHRR NDVI time series in Western Argentina
Authors: Loyarte María M. González , Menenti Massimo , Diblasi Angela M. 
Published: August 2007

AbstractThis study assessed whether the relationship of climate with foliar phenology is sufficiently robust to use a measure of foliar phenology to interpolate climate statistics in areas where observations are sparse. The normalized difference vegetation index (NDVI) was used to represent vegetation activity. As a measure of foliar phenology, we used parameters obtained by modelling NDVI time series with a Fast Fourier Transform (FFT) applied to a 9‐year time series of monthly National Oceanographic and Atmospheric Administration (NOAA) advanced very high resolution radiometer (AVHRR) NDVI global area coverage (GAC) images. The FFT decomposes the series into an average signal and to sinusoidal components. The selected FFT parameters were mean NDVI, and amplitude and phase for a 1‐year period. Our specific objective was to relate the ratio of precipitation, P, over potential evapotranspiration, ETP, to the FFT parameters in two complementary ways. The first was to use them as attributes in a numerical classification to obtain a map of foliar isophenology, and then associate these classes with bioclimatic types, thus generating a bioclimatic map. The second was to fit a multiple linear regression model with P/ETP as predicted variable and the FFT parameters as predictive variables. The regression model was then applied to obtain a map of the ratio P/ETP. The latter gave a second bioclimatic map. Foliar isophenology classes show a north–south decrease in phase value and increase in amplitude and mean NDVI values, thus reflecting the transition in climate conditions from hotter and drier to wetter and cooler. The model explains 92% (p‐value < 10−12) of the spatial variation in the P/ETP ratio. When using a single FFT parameter, no significant relationship was obtained. The three parameters provide complementary information to understand phenological variability in response to climate variability. Modelling bioclimate by means of monthly NDVI series summarized by Fourier analysis is an adequate tool to extend climate data where they are sparse. Copyright © 2007 Royal Meteorological Society
The effects of RANKL inhibition on fracture healing and bone strength in a mouse model of osteogenesis imperfecta
Authors: Delos Demetris , Yang Xu , Ricciardi Benjamin F. , Myers Elizabeth R. , Bostrom Mathias P.G. , Camacho Nancy Pleshko 
Published: August 2007

AbstractCurrently, the standard treatment for osteogenesis imperfecta (OI) is bisphosphonate therapy. Recent studies, however, have shown delayed healing of osteotomies in a subset of OI patients treated with such agents. The current study sought to determine the effects of another therapy, RANKL inhibition, on bone healing and bone strength in the growing oim/oim mouse, a model of moderate to severe OI. Mice [73 oim/oim and 69 wild‐type (WT)] were injected twice weekly with either soluble murine RANK (RANK‐Fc) (1.5 mg/kg) or saline beginning at 6 weeks of age. At 8 weeks of age, the animals underwent transverse mid‐diaphyseal osteotomies of the right femur. Therapy was continued until sacrifice at 2, 3, 4, or 6 weeks postfracture. At 6 weeks post‐fracture, greater callus area (6.59 ± 3.78 mm2 vs. 2.67 ± 2.05 mm2, p = 0.003) and increased radiographic intensity (mineral density) (0.48 ± 0.14 vs. 0.30 ± 0.80, p = 0.005) were found in the RANK‐Fc versus saline oim/oim group, indicating a delay in callus remodeling. Despite this delay, mechanical tests at 6 weeks postfracture revealed no significant differences in whole bone properties of stiffness and failure moment. Further, RANKL inhibition resulted in a greater failure moment and greater work to failure for the nonfractured contralateral WT bones compared to the nonfractured saline WT bones. Together, these results demonstrate that RANKL inhibition does not adversely affect the mechanical properties of healing bone in the oim/oim mice, and is associated with increased strength in intact bone in the WT mice. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:153–164, 2008
Sentinel node biopsy in oral squamous cell carcinoma
Authors: Civantos Francisco , Zitsch Robert , Bared Anthony 
Published: August 2007

AbstractThe clinical utility of sentinel node biopsy techniques for cutaneous melanoma has led multiple investigators to study the applicability of this approach to other solid tumors, including cancers of the upper aerodigestive tract, and especially the oral cavity. Preliminary data indicate that it may be useful for early oral cancers, with the exception of floor of mouth tumors, where technical challenges related to the proximity of the lymphatic basin remain a problem. A multi‐institutional pathologic validation trial, involving sentinel node biopsy followed by completion selective neck dissection, has completed accrual. While central step sectioning and immunohistochemistry remain to be completed and analyzed, routine pathologic techniques provided negative predictive values of 96% for oral cancer excluding floor of mouth lesions. Subsequent trials need to involve clinical follow‐up and evaluation for recurrence in the neck. We believe this technique may ultimately play a role in the management of early oral cancer. J. Surg. Oncol. 2007;96:330–336. © 2007 Wiley‐Liss, Inc.
Sentinel lymph node biopsy for breast cancer
Authors: Sato K. , Shigenaga R. , Ueda S. , Shigekawa T. , Krag D.N. 
Published: August 2007

AbstractThis manuscript is a brief discussion of the developments of the technology and concepts that led to modern procedures of radiotracer guided surgery of sentinel nodes (SNs) for breast cancer. The past section highlights some of the contributions by key persons involved with SN methods. The present section describes the magnitude of types of published material to date. The future section describes the major international trials and some important technical challenges yet to be solved. J. Surg. Oncol. 2007;96:322–329. © 2007 Wiley‐Liss, Inc.
Operative probe scintimetry with indium and technetium for colorectal cancer
Authors: Kuhn Joseph A. , Nochumson Joshua 
Published: August 2007

AbstractSurgeons introduced the hand‐held gamma detection probe in combination with tumor‐directed monoclonal antibodies in patients with colorectal cancer. The clinicians conducted innovative research involving antibody chemistry and variation as well as radioactive dosimetry and decay. The results of these studies represented an era when surgeons began reporting on specific lesion detection and the impact of the antibody administration on the management of the patient. The summary of the important early trials involving monoclonal antibodies and probe scintimetry provides a valuable look into the early development of the antibody technology and a glimpse of potential future applications using the gamma detection probe. J. Surg. Oncol. 2007;96:290–296. © 2007 Wiley‐Liss, Inc.
The real effects of inflation in continuous versus discrete time sticky price models
Authors: Alex Ho Wai‐Yip , Yetman James 
Published: August 2007

AbstractWe demonstrate the important implications of the assumptions of discrete time in many sticky price models of the macroeconomy. For a given level of menu costs, discrete time models imply longer average contract length but smaller real effects of both trend inflation and monetary shocks than continuous time models. It is also feasible for a firm to enjoy full price flexibility in discrete time, while this would require paying infinite menu costs in continuous time, a distinction that is most important at high levels of trend inflation. Copyright © 2007 John Wiley & Sons, Ltd.
J‐modulated ADEQUATE experiments using different kinds of refocusing pulses
Authors: Thiele Christina M. , Bermel Wolfgang 
Published: August 2007

AbstractOwing to the recent developments concerning residual dipolar couplings (RDCs), the interest in methods for the accurate determination of coupling constants is renascenting. We intended to use the J‐modulated ADEQUATE experiment by Kövér et al. for the measurement of 13C13C coupling constants at natural abundance. The use of adiabatic composite chirp pulses instead of the conventional 180° pulses, which compensate for the offset dependence of 13C 180° pulses, led to irregularities of the line shapes in the indirect dimension causing deviations of the extracted coupling constants. This behaviour was attributed to coupling evolution, during the time of the adiabatic pulse (2 ms), in the J‐modulation spin echo. The replacement of this pulse by different kinds of refocusing pulses indicated that a pair of BIPs (broadband inversion pulses), which behave only partially adiabatic, leads to correct line shapes and coupling constants conserving the good sensitivity obtained with adiabatic pulses. Copyright © 2007 John Wiley & Sons, Ltd.
Dependence of 35Cl NQR on hydrogen bonding and temperature in dichlorophenol–aniline charge transfer complexes
Authors: Ramananda D. , Ramesh K. P. , Uchil J. 
Published: August 2007

AbstractThe hydrogen‐bonded charge transfer complexes of aniline with π‐acceptors (or proton donors) such as 2,5‐, 2,6‐, 3,4‐ and 3,5‐dichlorophenol were prepared. The 35Cl nuclear quadrupole resonance (NQR) frequencies of these charge transfer complexes in the temperature range 77–300 K were measured to ascertain the existence or otherwise of a phase transition upon complex formation. Further, the NQR frequency and asymmetry parameter of the electric field gradient at the site of quadrupole nucleus were used to estimate the chemical bond parameters, namely ionic bond, double bond character of the carbon–chlorine(CCl) bond and the percentage charge transfer between the donor–acceptor components in charge transfer complexes. The effect of hydrogen bonding and temperature on the charge transfer process is analysed. Copyright © 2007 John Wiley & Sons, Ltd.
Complete assignments of 1H and 13C NMR spectral data for arylnaphthalene lignan lactones
Authors: da Silva Rosangela , Maia Ruas Marcelo , Marcos Donate Paulo 
Published: August 2007

AbstractIn this work we describe the complete 1H and 13C NMR analyses of three arylnaphtalene lignan lactones (taiwanin C, 4‐methyl dehydroretrodendrin and justicidin B) using modern NMR techniques such as gCOSY, nonedited gHSQC, gHMBC and NOE experiments. Complete assignment and homonuclear hydrogen coupling constant measurements were performed. Copyright © 2007 John Wiley & Sons, Ltd.
Analytical time integration for BEM axisymmetric acoustic modelling
Authors: Warszawski A. , Mansur W. J. , Soares D. 
Published: August 2007

AbstractIn this work, a numerical time‐domain approach to model acoustic wave propagation in axisymmetric bodies is developed. The acoustic medium is modelled by the boundary element method (BEM), whose time convolution integrals are evaluated analytically, employing the concept of finite part integrals. All singularities for space integration, present in the expressions generated by time integration, are treated adequately. Some applications are presented to demonstrate the validity of the analytical expressions generated for the BEM, and the results obtained with the present approach are compared with those generated by applying numerical time integration. Copyright © 2007 John Wiley & Sons, Ltd.
Treatment of wild‐type gastrointestinal stromal tumor (WT‐GIST) with imatinib and sunitinib
Authors: Murray Matthew , Hatcher Helen , Jessop Flora , Williams Denise , Carroll Nicholas , Bulusu Ramesh , Judson Ian 
Published: August 2007

AbstractWe report a rare case of advanced, metastatic gastrointestinal stromal tumor (GIST) in a young female. Molecular analysis of the tumor revealed wild‐type (WT) KIT and platelet derived growth factor receptor alpha (PDGFRA) gene status with no mutations characteristic of adult GIST. Despite this she had clinical benefit and evidence of radiological response to sequential treatment with the tyrosine kinase inhibitors imatinib and sunitinib. Pediatr Blood Cancer 2008;50:386–388. © 2007 Wiley‐Liss, Inc.
Timing of menarche among survivors of childhood acute lymphoblastic leukemia: A report from the Childhood Cancer Survivor Study
Authors: Chow Eric J. , Friedman Debra L. , Yasui Yutaka , Whitton John A. , Stovall Marilyn , Robison Leslie L. , Sklar Charles A. 
Published: August 2007

AbstractBackgroundThe objective of this study was to determine risk factors associated with abnormal timing of menarche among survivors of childhood acute lymphoblastic leukemia (ALL).ProcedureSelf‐reported age of menarche was determined among 949 female ALL survivors participating in the Childhood Cancer Survivor Study (CCSS), a cohort of 5‐year survivors of common pediatric cancers diagnosed from 1970 to 1986, and compared with 1,128 siblings.ResultsThe majority of survivors (92%) and siblings (97%) reported menarche between the ages of 10 and 16. Survivors treated with chemotherapy alone, including those exposed to alkylating agents, experienced menarche at a similar rate to siblings. However, compared to chemotherapy alone, cranial radiotherapy was associated with early menarche (age < 10; OR 6.2, 95% CI 2.1, 18.5) while craniospinal radiotherapy was associated with both early (OR 8.6, 95% CI 1.9, 38.6) and late (age > 16; OR 4.8, 95% CI 1.4, 16.7) menarche. There were no differences in effect between <20 and ≥20 Gy radiotherapy doses. In multivariable analysis, younger age at diagnosis was an independent risk factor for early menarche.ConclusionsFew female childhood ALL survivors experienced menarche outside of the normal range. Alkylating agent exposure was not associated with abnormal timing. However, those exposed to cranial and craniospinal radiotherapy, especially at a young age, should be monitored closely for abnormal timing of menarche. Pediatr Blood Cancer 2008;50:854–858. © 2007 Wiley‐Liss, Inc.