bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral R115866 in the treatment of moderate to severe plaque‐type psoriasis
Authors: Verfaille CJ , Thissen CACB , Bovenschen HJ , Mertens J , Steijlen PM , Van De Kerkhof PCM 
Published: March 2007

AbstractBackground R115866 (RambazoleTM) is a new generation all‐transretinoic acid metabolism blocking agent, highly specific against the retinoic acid 4‐hydroxylase. The drug alleviates hyperproliferation and normalizes differentiation of the epidermis in animal models of psoriasis.Objective To explore the efficacy, safety and tolerability of systemic R115866 in patients with moderate to severe plaque‐type psoriasis.Patients and methods  In this open label, single‐arm trial, patients were treated with R115866, 1 mg/day for 8 weeks, followed by a 2‐week treatment‐free follow‐up period. Patients were monitored for efficacy and safety.Results Nineteen patients (intent‐to‐treat population) were treated and 14 completed the entire study. Two patients discontinued due to lack of efficacy and three due to adverse events. At the end of the treatment, 26% of the patients showed at least 50% reduction in Psoriasis Area Severity Index (PASI) compared to baseline. Further improvement was observed at the end of the 2‐week follow‐up period where 47% of the patients showed a 50% or greater reduction in PASI. Kinetic data showed no evidence of accumulation of either R115866 or retinoic acid in plasma. The most common adverse events were pruritus, xerosis, cheilitis and an increase in blood triglycerides. The majority of adverse events were mild to moderate. No deaths or serious adverse events were reported.Conclusion Eight‐week daily treatment with 1 mg R115866 resulted in a significant reduction in PASI from baseline to end of therapy. Additional improvement was seen after the 2‐week follow‐up period. The drug was well tolerated. R115866 merits further evaluation to optimize its clinical efficacy and safety profile in moderate to severe plaque‐type psoriasis.
Human papillomavirus (HPV) viral load and HPV type in the clinical outcome of HIV‐positive patients treated with imiquimod for anogenital warts and anal intraepithelial neoplasia
Authors: Sanclemente G , Herrera S , Tyring SK , Rady PL , Zuleta J‐J , Correa L‐A , He Q , Wolff J‐C 
Published: March 2007

AbstractObjective  To evaluate the efficacy of 5% imiquimod in HIV‐positive male patients with anogenital warts or anal intraepithelial neoplasia (AIN), and to elucidate whether human papillomavirus (HPV) type and viral load were important for clinical outcome and recurrences.Methods  Thirty‐seven patients with histologically proven anogenital warts or AIN were enrolled. Topical 5% imiquimod was applied three times per week for more than 8 h overnight for 16 weeks, although patients were allowed to continue therapy for 4 more weeks if they did not have complete clearance of lesions.Results  Mean age was 34 years. The perianal area was the main lesion location. Thirty‐three patients had CD4 counts of < 500 cells/mm3. Eighteen patients had a histopathological diagnosis of AIN‐1. Main HPV types detected corresponded to low‐risk HPV types. At 20 weeks of therapy, 46% patients achieved total clearance whereas 14 patients had > 50% clearance. Recurrence was observed in 5 of 17 patients who cleared. Clearance was not influenced by patients’ CD4 counts, wart location, HIV viral load or HPV viral load.Conclusions  The assumption that visible perianal warts are benign lesions in HIV‐positive patients has to be reevaluated since an important number of such lesions could correspond to low‐grade anal disease, which in turn could progress to high‐grade anal disease or cancer. In addition, our results in this preliminary study indicate that imiquimod appears to be effective in treating AIN in HIV‐positive patients. Further studies are needed to document its utility to prevent high‐grade dysplasia and/or anal cancer.
Screening for asymptomatic carriage of Trichophyton tonsurans in household contacts of patients with tinea capitis: results of 209 patients from South London
Authors: White JML , Higgins EM , Fuller LC 
Published: March 2007

AbstractBackground  There is currently an epidemic of tinea capitis in urban areas of developed countries caused by Trichophyton tonsurans. Recurrence or re‐infection with dermatophyte is not uncommon after adequate oral treatment. Asymptomatic carriers who are household contacts may partly explain this observation by forming a reservoir for infection.Patients/methods  Two‐hundred and nine household contacts of patients with tinea capitis were examined and screened for asymptomatic carriage of dermatophyte.Results  Only 7.2% had clinically evident disease yet 44.5% had silent fungal carriage on the scalp. Children under 16 years were much more likely to be carriers than adults (P < 0.001) and males were less likely than females to be affected (P < 0.01).Conclusion  This evidence poses questions about factors relevant in transmission of dermatophytes. The authors propose that all household contacts of patients with tinea capitis should be offered screening to eradicate a potential reservoir of infection.
A confusing world: what to call histology of three‐dimensional tumour margins?
Authors: Moehrle M , Breuninger H , Röcken M 
Published: March 2007

AbstractComplete three‐dimensional histology of excised skin tumour margins has a long tradition and, unfortunately, a multitude of names as well. Mohs, who introduced it, called it ‘microscopically controlled surgery’. Others have described it as ‘micrographic surgery’, ‘Mohs’ micrographic surgery’, or simply ‘Mohs’ surgery’. Semantic confusion became truly rampant when variant forms, each useful in its own way for detecting subclinical outgrowths of malignant skin tumours, were later introduced under such names as histographic surgery, systematic histologic control of the tumour bed, histological control of excised tissue margins, the square procedure, the perimeter technique, etc.All of these methods are basically identical in concept. All involve complete, three‐dimensional histological visualization and evaluation of excision margins. Their common goal is to detect unseen tumour outgrowths.For greater clarity, the authors of this paper recommend general adoption of ‘3D histology’ as a collective designation for all the above methods. As an added advantage, 3D histology can also be used in other medical disciplines to confirm true R0 resection of, for example, breast cancer or intestinal cancer.
Isolation and characterization of microsatellite loci in Pseudoplatystoma corruscans (Siluriformes: Pimelodidae) and cross‐species amplification
Authors: REVALDAVES ELOISA , PEREIRA LUIZ H. G. , FORESTI FAUSTO , OLIVEIRA CLAUDIO 
Published: March 2007

AbstractA total of five polymorphic microsatellites loci from Pseudoplatystoma corruscans were isolated and characterized. A population survey involving 43 specimens resolved a large number of alleles (range seven to eight among loci) and high observed heterozygosity (0.500–0.615), indicating its usefulness in population genetics studies. Cross‐species amplification was successful in four other Pimelodidae species.
Polymorphic microsatellite loci in the parasitic wasp Cotesia glomerata (Hymenoptera: Braconidae)
Authors: ZHOU Y. , GU H. , DORN S. 
Published: March 2007

AbstractFive polymorphic microsatellite loci were isolated in a gregarious endoparasitoid, Cotesia glomerata, using an enrichment protocol. We detected two to 15 alleles per locus in a sample of 50 female wasps collected from a field population near Zürich, Switzerland. The observed heterozygosities ranged from 0.21 to 0.92. These markers are currently being used in our studies of sex determination in this species.
Isolation of polymorphic tetranucleotide microsatellite markers for the pygmy kingfisher Ceyx picta
Authors: BARDELEBEN CAROLYNE , GRAY MELISSA M. 
Published: March 2007

AbstractWe have isolated 13 polymorphic microsatellite markers for the pygmy kingfisher, Ceyx picta, from genomic libraries enriched for either (AAGG)n or (AACC)n repetitive elements. The number of alleles range from three to 15 per locus with an observed heterozygosity ranging from 0.46 to 1.00. These markers will be useful for analysis of questions concerning population genetic structure and models of speciation.
Large scale development of selectively amplified microsatellite polymorphic loci (SAMPL) markers in spruce (Picea)
Authors: GUPTA A. K. , KANG B. Y. , ROY J. K. , RAJORA O. P. 
Published: March 2007

AbstractThe spruce (Picea) species are ecologically and economically important in Canada. Highly informative markers with high multiplex ratios are needed to assist spruce genomics, genetics, and breeding programs. Selectively amplified microsatellite polymorphic loci (SAMPL) markers are highly suitable for these programs. We have developed, optimized, and characterized a set of 10 new SAMPL primers in combination with 16 MseI primers and resolved a large number of polymorphic SAMPL markers in spruce. The SAMPL primers were designed from the compound microsatellite repeats found in Norway spruce (Picea abies) and white spruce (Picea glauca). A total of 6313 polymorphic SAMPL makers were produced by 160 SAMPL–MseI primers combinations in eight progeny of a spruce mapping population.
Development and characterization of microsatellite markers for the poplar rust fungi Melampsora medusae and Melampsora larici‐populina
Authors: STEIMEL J. , CHEN W. , HARRINGTON T. C. 
Published: March 2007

Abstract Melampsora species and their hybrids are obligate parasites of Populus and Salix species worldwide. The increasing interest in Populus and Salix for biomass and fibre production necessitates genetic markers for population studies of Melampsora spp. Libraries enriched for simple sequence repeats were used to develop five microsatellite markers for Melampsora medusae and Melampsora larici‐populina. The variation detected by these markers will be valuable for phylogenetic and population genetic studies, substantiate putative hybrids, and deployment of resistant poplar clones.
Development and characterization of microsatellite markers for Prosopis chilensis and Prosopis flexuosa and cross‐species amplification
Authors: MOTTURA M. C. , FINKELDEY R. , VERGA A. R. , GAILING O. 
Published: March 2007

Abstract Prosopis chilensis and Prosopis flexuosa (Fabaceae) are closely related hardwood arboreal species that are widely distributed in the arid regions of Argentina. The development of highly polymorphic markers, such as microsatellites, is desirable for genetic studies of these species. Here, we present the development and characterization of six polymorphic microsatellite markers in P. chilensis and P. flexuosa. These markers showed a polymorphism information content between 0.14 and 0.85 and the number of alleles varied from two to 13 considering both species. All markers revealed a broad cross‐species affinity when tested in seven other Prosopis species. All primers amplified in at least five species.
Characterization of polymorphic microsatellite loci in the aphid species Macrosiphum euphorbiae (Hemiptera: Aphididae)
Authors: RABOUDI F. , CHAVIGNY P. , MARRAKCHI M. , MAKNI H. , MAKNI M. , VANLERBERGHE‐MASUTTI F. 
Published: March 2007

AbstractFourteen microsatellite loci were isolated and characterized in the potato aphid, Macrosiphum euphorbiae Thomas, by screening a genomic library with the oligonucleotide probes (GA)10 (GT)10 and (GATA)4. Allelic diversity was estimated in samples collected from potato fields in Tunisia. Ten loci displayed polymorphism that ranged from two to four alleles per locus and the observed heterozygosity ranged from zero to one. These markers could be used to study the population genetic structure of this polyphagous aphid species.
Isolation and characterization of microsatellite markers in Bonelli's eagle (Hieraaetus fasciatus)
Authors: MIRA S. , WOLFF K. , CANCELA M. L. 
Published: March 2007

AbstractTo obtain polymorphic molecular markers for population genetics and conservation studies in Bonelli's eagle populations, we screened a partial genomic library enriched for microsatellites with di‐ and tri‐nucleotide motifs [(GT) (CT) (AAC) and (GCC)]. A total of 15 polymorphic markers were obtained. The number of alleles ranged from two to eight. These markers will be very useful for paternity tests and population structure studies as well as for evaluating the outcome of conservation programs.
Isolation and characterization of microsatellite loci from the entomopathogenic hyphomycete, Paecilomyces fumosoroseus
Authors: DALLEAU‐CLOUET C. , GAUTHIER N. , RISTERUCCI A.‐M. , BON M.‐C. , FARGUES J. 
Published: March 2007

AbstractNine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26 P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed number of alleles ranging from two to six. The loci differentiated some isolates and group of isolates according to their geographical location, showing promise for the study of gene flow. All loci failed to give clear amplifications in P. fumosoroseus isolates from hosts other than B. tabaci. These microsatellite markers provide powerful tools for ecological, epidemiological, and population genetic studies.
Di‐ and tri‐nucleotide repeat microsatellites for the mealy plum aphid, Hyalopterus pruni
Authors: LOZIER J. D. , MILLS N. J. , PALSBØLL P. J. , RODERICK G. K. 
Published: March 2007

Abstract Hyalopterus pruni is an invasive aphid pest in California. To study the population biology of this pest both in California and its native Mediterranean region, we have developed 11 di‐ and tri‐nucleotide repeat microsatellite markers. Each locus amplified in individuals representing the full range of geographical regions and host plants where Hyalopterus is found. Polymorphism was high, ranging from six to 22 alleles per locus in the individuals screened. These loci represent the first microsatellites developed for Hyalopterus and they should be of great value in studying the invasion biology and population structure of this insect pest.
Polymorphic microsatellite loci in the bush‐cricket Metrioptera roeseli
Authors: HOLZHAUER STEPHANIE I. J. , WOLFF KIRSTEN 
Published: March 2007

Abstract Metrioptera roeseli is a common bush‐cricket in Europe which prefers grassland as habitat. We developed microsatellite loci in order to study the population genetic structure and the effects of different habitat types on the genetic structure of the bush‐cricket in a rural landscape. In this paper, we report on six polymorphic microsatellite loci with seven to 16 alleles per locus in 20 individuals from the Lahn‐Dill‐Bergland in Hesse, Germany.
Characterization of polymorphic microsatellite loci in the terrestrial isopod Porcellionides pruinosus
Authors: FRÉDÉRIC GRANDJEAN , NICOLAS GOUIN , SÉBASTIEN VERNE , CARINE DELAUNAY , SYLVIE PATRI 
Published: March 2007

AbstractWe developed a set of six polymorphic microsatellite loci for the woodlouse, Porcellionides pruinosus. We screened 43 individuals from three French populations and found that locus‐specific allelic diversity ranges from 3 to 17 alleles (mean = 9.8) and observed heterozygosity ranges from 0.51 to 0.89 (mean = 0.77). These are the first microsatellites loci reported from the woodlouse.
Isolation and characterization of nuclear microsatellite loci in Lupinus group Microcarpi (Leguminosae)
Authors: DRUMMOND C. S. , HAMILTON M. B. 
Published: March 2007

AbstractMicrosatellite loci are described for Lupinus group Microcarpi (Leguminosae), an assemblage of annual plants primarily inhabiting western North America. In all, 188 unique microsatellite loci were isolated, and seven loci (averaging 9.6 alleles each) have been developed as polymorphic markers for estimating population structure and outcrossing rates in Lupinus microcarpus var. densiflorus and var. horizontalis. Departures from Hardy–Weinberg expected frequencies of heterozygotes are consistent with structured populations and autogamous or mixed mating systems. Several loci were amplified successfully in other species of Lupinus, demonstrating the potential utility of these markers beyond group Microcarpi.
Characterization of 16 polymorphic microsatellite loci in weathervane scallop Patinopecten caurinus
Authors: ELFSTROM C. M. , SMITH C. T. , JONES K. C. , SEEB J. E. 
Published: March 2007

AbstractDiscrete and commercially important patches of weathervane scallops (Patinopecten caurinus) are found in the northeastern Pacific Ocean. Little is known about weathervane scallop life history and population structure, but the species is vulnerable to overexploitation because of their large size and long lifespan. Here we describe 16 polymorphic microsatellite loci developed to study the relatedness of these discrete patches. All loci were polymorphic in the 32 individuals tested; the number of alleles range from four to 26 and heterozygosities ranged from 0.437 to 1.000.
Isolation and characterization of microsatellite loci fromSilene tatarica
Authors: TERO N. , SCHLÖTTERER C. 
Published: March 2007

AbstractFive new microsatellite loci were isolated from the perennial plant,Silene tatarica. We characterizedS. tataricaindividuals originating from two riverbank populations in northern Finland and observed between four and nine alleles per locus. Observed heterozygosity was consistently lower than gene diversity (HO: 0.0450–0.2385,HE: 0.1919–0.6187). This deficiency of heterozygous genotypes was observed in most locus/population combinations, and is presumably caused by spatial genetic structuring due to restricted seed flow within subpopulations. The markers presented here are the first microsatellites reported forS. tatarica.
Characterization of microsatellite markers in the shea tree (Vitellaria paradoxa C. F Gaertn) in Mali
Authors: CARDI CÉLINE , VAILLANT ALEXANDRE , SANOU HABY , KELLY BOKARY A. , BOUVET JEAN‐MARC 
Published: March 2007

AbstractIn order to study the genetic diversity and structure in the population of Vitellaria paradoxa, we characterized eight polymorphic microsatellite loci. Primers to amplify these loci were tested on 169 individual trees representing a sample of the population of shea tree in Mali. The loci were all polymorphic with a number of alleles between three to nine and with observed level of heterozygosity ranging from 0.035 to 0.507. These markers will be useful for genetic and ecological studies of this species.
Development and characterization of 11 microsatellite markers in a widespread Neotropical seasonally dry forest tree species, Geoffroea spinosa Jacq. (Leguminosae)
Authors: NACIRI‐GRAVEN Y. , CAETANO S. , PRADO D. , PENNINGTON R. T. , SPICHIGER R. 
Published: March 2007

AbstractEleven dinucleotide microsatellites were developed in Geoffroea spinosa (Leguminosae), a widespread tree of the seasonally dry Neotropical forests, and characterized on six populations from Peru, Argentina and Paraguay. Four of them amplified on the Peruvian populations only, probably because of mutations in the microsatellite flanking regions in the other populations. Ten microsatellites were found polymorphic, with within population gene diversities ranging from 0.17 to 0.95, and a number of alleles varying from seven to 19. A significant overall genetic differentiation was also found (θ = 0.212; P < 0.01).
Isolation and characterization of microsatellite loci from the barley scald pathogen, Rhynchosporium secalis
Authors: LINDE CELESTE C. , ZALA MARCELLO , MCDONALD BRUCE A. 
Published: March 2007

AbstractFifteen primer pairs were designed for 14 polymorphic microsatellite loci, which were isolated and characterized from genomic libraries of Rhynchosporium secalis. Conditions for multiplexing and simultaneous genotyping of up to eight loci in a single run are described. The number of alleles per polymorphic locus ranged from two to 13 in populations from Switzerland and Australia. Genotypic diversity ranged from 61.5 to 66.7. Gene diversity ranged from 0.08 to 0.89 for individual polymorphic loci, with averages of 0.54 and 0.62 for the populations from Switzerland and Australia, respectively. Variable levels of polymorphism make these ideal markers for population genetic analyses.
Development and characteristics of microsatellite markers in Pinus merkusii
Authors: NURTJAHJANINGSIH I. L. G. , SAITO Y. , LIAN C. L. , TSUDA Y. , IDE Y. 
Published: March 2007

Abstract Pinus merkusii is an important industrial species that is distributed only in Southeast Asia. We isolated 10 microsatellite markers from this species using a dual‐suppression‐polymerase chain reaction technique. Of these markers, five loci were codominant and polymorphic. The number of alleles per locus ranged from three to six and the expected heterozygosity ranged from 0.389 to 0.728. These microsatellite markers will be available for analysis on population genetics and mating patterns.
Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae)
Authors: POULAKAKIS N. , GOULIELMOS G. , ANTONIOU A. , ZOUROS E. , MYLONAS M. 
Published: March 2007

AbstractFive polymorphic microsatellite loci (containing di‐ and penta‐nucleotide repeats) were developed for the Erhard's wall lizard, Podarcis erhardii, an endemic species of southern Balkan Peninsula. The number of alleles ranged from five to 17. Observed and expected heterozygosities ranged from 0.28 to 0.92 and 0.57 to 0.91, respectively. These markers will provide a valuable tool for population genetics analyses and can contribute to the evaluation of conservation programs.
Application of the nonradioactive biotin–streptavidin system to visualize AFLP fragments
Authors: PFEIFFER TANJA , ZIPPEL ELKE , FRITZ SEBASTIAN , STECH MICHAEL 
Published: March 2007

AbstractAmplified fragment length polymorphism (AFLP) fingerprinting is one of the most widely used molecular techniques in plant biology today. In this paper, we describe the application of the extremely sensitive nonradioactive biotin–streptavidin system to visualize AFLP fragments blotted onto nylon membranes. The protocol is tested for different plant species (from bryophytes, gymnosperms and angiosperms) and primer combinations. Advantages of this protocol over other nonradioactive detection methods are discussed, and the suitability of the method for laboratories without automated sequencing facilities are emphasized.
Five polymorphic microsatellite markers for the study of Cardiocondyla elegans (Hymenoptera: Myrmicinae)
Authors: LENOIR JEAN‐CHRISTOPHE , SCHREMPF ALEXANDRA , LENOIR ALAIN , HEINZE JÜRGEN , MERCIER JEAN‐LUC 
Published: March 2007

AbstractWe describe primer sequences for five microsatellite markers in Cardiocondyla elegans, an ant species with ergatoid males. Polymorphism of these loci was investigated using 236 individuals from 22 colonies from four locations. The microsatellites are dinucleotide repeats with four to 16 alleles, and the observed heterozygosity ranges from 0.244 to 0.720. We characterized these markers for the study of the population as well as the social structure of colonies.
New microsatellite loci confirm hybrid origin, parthenogenetic inheritance, and mitotic gene conversion in the gynogenetic Amazon molly (Poecilia formosa)
Authors: TIEDEMANN R. , MOLL K. , PAULUS K. B. , SCHLUPP I. 
Published: March 2007

AbstractWe describe the first microsatellite loci for the gynogenetic Amazon molly, Poecilia formosa, an all‐female species arisen through hybridization of the bisexual species Poecilia mexicana and Poecilia latipinna. The loci showed one to six alleles and an expected heterozygosity between zero and 0.75. As expected with parthenogenetic inheritance, most loci were either constantly homozygous (five loci) or constantly heterozygous (eight loci). For six loci, both heterozygotes and homozygotes occurred. This and the fact that some loci only showed alleles of one of the ancestral species could indicate genome homogenization through mitotic gene conversion. Our new loci conformed to the hybrid origin of Amazon molly and are also applicable to both ancestral bisexual species.