bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal Relationship Between Metabolites and Vasomotor Symptoms in Menopause: A Two-Sample Mendelian Randomization Analysis Study
Authors: Zhongyu Huang , Zipan Lyu , Huazhi You , Yaojuan He , Lijuan Li , Shaonan Li , Feng Shen , Li Yanfang 
Published: April 2024

Abstract

Objective:
Perimenopause represents a critical phase in a woman's reproductive journey characterized by a myriad of challenges, with vasomotor symptoms (VMS) emerging as a significant hurdle. This study endeavors to explore the potential causal associations between metabolites and VMS during perimenopause, aiming to elucidate the biological processes influencing VMS manifestation and inform personalized therapeutic strategies.
Methods:
Leveraging a two-sample Mendelian randomization (MR) analysis, comprehensive genome-wide association studies (GWAS) datasets were utilized to discern specific metabolites implicated in VMS etiology adhering to STROBE-MR guidelines. The primary metabolite dataset, derived from the metabolite GWAS, includes genetic association evidence for 486 metabolites across 7,824 individuals. VMS GWAS summary statistics, with 14,261 cases and 77,767 controls, form the basis for causal inference. Rigorous quality control measures ensure the integrity of associations, and assumptions crucial to MR analysis are addressed, including relevance, independence, and exclusion restriction. Protein-protein interaction(PPI) network and enrichment analysis were constructed to explore potential regulatory pathways associated with VMS.
Results:
MR analysis identified 12 metabolites associated with VMS among which mannose, isoleucine, stearate, myristate, ascorbate, 3-dehydrocarnitine, 4-androsten-3beta,17beta-diol disulfate 1 and 10-undecenoate show inverse associations, suggesting potential protective effects. Conversely, inosine, myristate, gamma-glutamyl leucine, glucose, and serine display positive associations, indicating contributory effects in pathogenesis. Biological network analysis elucidated the molecular pathways linking these metabolites to VMS, highlighting the involvement of diverse biological processes such as neuroactive ligand-receptor interaction, lipid metabolism, hormonal responses, and immune modulation. Furthermore, protein-protein interaction networks identified hub genes central to VMS regulation, including MME, CCND1, TNF, ITGB1, PTGS2, HSP90AA1, CTSB, PPARG, PPARA, and CXCL8, shedding light on potential regulatory mechanisms.
Conclusion:
This study highlighted the complexity of perimenopausal VMS and the importance of holistic therapies. Targeted interventions focusing on specific metabolites and pathways served as potential approach for symptom relief. Acknowledging limitations in analyses and datasets, future research should prioritize diverse cohorts to enhance understanding and treatment of VMS.
Causal Associations between Prostate Cancer and Various Hematological Parameters: Insights from Multivariable and Bidirectional Mendelian Randomization Analyses
Authors: Wei Yirui , Yang Pushen , Wang Hao , Xie Dawei , He Weifeng , Wang Jianwen 
Published: April 2024

Abstract
Purpose This study aims to investigate the causal relationship between hematologic parameters and the risk of developing PCa, employing a rigorous and methodical scientific approach.
Methods We applied genome-wide association studies (GWAS) to conduct both forward and reverse Mendelian randomization (MR) analyses within a two-sample framework. We further undertook multivariable MR analyses to investigate the associations between diverse hematological parameters and PCa. To validate the robustness of our results, we implemented several sensitivity analysis techniques.
Results In our univariable MR analysis, genetically predicted mean corpuscular volume (Odds Ratio [OR]: 0.942, 95% Confidence Interval [CI]: 0.891–0.996, P=0.035) and mean corpuscular hemoglobin (OR: 0.934, 95% CI 0.882–0.988, P=0.018) in red blood cells were associated with a decreased risk of PCa. Moreover, MR analysis revealed that genetically predicted increases in eosinophil count (OR: 1.081, 95% CI 1.005–1.163, P=0.036) and basophil count (OR: 1.235, 95% CI 1.006–1.516, P=0.044) were linked to an elevated risk of PCa (all P<0.05). In the multivariable analysis, we found that basophil count was associated with an increased incidence of PCa (OR:1.432, 95% CI 1.028–1.996, P=0.034). In the reverse MR analysis, we observed that genetically predicted PCa was associated with an increase in neutrophil count (OR: 1.012, 95% CI: 1.001–1.023, P=0.031) and red blood cell count (OR: 1.008, 95% CI: 1.000–1.016, P=0.042).
Conclusion In conclusion, our investigation elucidates the causal associations between specific hematological parameters and PCa. These insights contribute significantly to our understanding of the genetic determinants of PCa and their potential interactions with various hematological parameters.
Healthcare monitoring and Recommendation model: Hybrid Deep learning Enabled Disease Diagnosis Framework
Authors: . Poonam , Mallapur Sujata V. 
Published: April 2024

Abstract
The continuous observation of patients’ health condition as well as monitoring varying crucial symptoms is termed as Healthcare monitoring, which also detect and trace any probable problems. This kind of observation is more crucial for detecting health conditions at early stage especially, recognizing cardiovascular disease in diabetes patient. However, diagnosing cardiovascular disease in diabetes patient is extremely difficult because of the small quantity of labeled data with the prevalence of outliers in the diabetes datasets. This paper proposes a novel healthcare monitoring and recommendation model with hybrid DL based disease diagnosis structure considering the heart disease. The improved z-score normalization is employed to preprocess the input data by scaling the numeric features within a general range. The BWSV-FE based feature extraction process is proposed to extract the entropy features; together with this feature, a raw feature and mutual information features are collected. Subsequently, the hybrid disease prediction model is proposed that includes improved Bi-GRU and LSTM models. Improved Bi-GRU is adopted for efficiently predicting the disease, in which the CCE is updated with the modified MSE loss function by inducing penalty function into this. Moreover, the penalty is computed by employing Gini Index function. Thereby, the hybrid disease prediction model efficiently predicts the diseases’ severity level that aids the doctor for guiding the management of the patients’ health.
The Influence of Professional Identification and Organizational Incentives on Turnover Intentions: A Hierarchical Linear Model in Beijing's Family Doctors
Authors: Zhai Ziyan , Cui Chengsen , Meng Kai 
Published: April 2024

Abstract
Introduction: The high turnover intentions among family doctos (FDs) in China have impacted the stability of teams and the quality of healthcare services in community health centers (CHCs). The factors influencing FDs' turnover intentions include not only individual characteristics but also organizational environmental factors within CHCs. This study aims to explore the mechanism of the impact of FDs' professional identification and organizational incentives on their turnover intentions. Methods: This study selected 3 397 FDs from 102 CHCs in six districts of Beijing as the research subjects. Multiple scales were used to quantify FDs' professional identity, turnover intention, and organizational incentives. A Hierarchical Linear Model (HLM) was employed to investigate the effects of organizational-level and individual-level factors on turnover intentions and to analyze the interaction between individuals and organizations. Results: The study found that FDs' professional identification has a significant negative impact on turnover intentions (Beta=-0.245, P < 0.001). The level of organizational incentives in CHCs had a significant negative impact on turnover intentions (Beta=-0.175, P < 0.001), and the level of organizational incentives played a significant negative moderating role in the process of individual professional identification influencing turnover intentions (Beta = 0.004, P < 0.05). Conclusions: Enhancing FDs' professional identity can lower their turnover intention, and the impact of personal identity on turnover intention diminishes in institutions with strong organizational incentives. During the training stage of FDs, it is essential to foster a strong personal professional identity value the role of organizational incentives, and optimize the overall organizational environment.
LncRNA MALAT1 regulates lymph node metastasis of triple-negative breast cancer through ceRNA mechanism
Authors: Yang Qiu-hui , Fu Ye-qin , Feng Wei-liang , Yu Xing-fei , Xu Ning , Yang Hong-jian , Zhang Xi-ping 
Published: April 2024

Abstract

Long non-coding RNA (LncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) plays an important role in the occurrence and development of cancer, but there are no relevant reports on the mechanism of MALAT1 in TNBC lymph node metastasis. Our study confirms that MALAT1 has a significant impact on the proliferation, invasion, and migration of TNBC cells, and MALAT1 may regulate TNBC cell metastasis through the competing endogenous RNA (ceRNA) mechanism of MALAT1/miR-106a-5p/REEP5 axis. In addition, prognostic analysis showed positive correlation between the expression level of MALAT1 and the degree of axillary lymph node metastasis in TNBC patients. This discovery is expected to provide new ideas for the diagnosis, and prognostic predicting of TNBC.
Evaluation of Cerebrospinal Fluid Flow by Nuclear Magnetic Resonance in Adolescent Idiopathic Scoliosis
Authors: ZAVARD Guillaume , VERCLYTTE Sébastien , CATALA Martin , AMBARKI Khalid , NORBERCIAK Laurène , CATANZARITI Jean-François 
Published: April 2024

Abstract

Objectives
Zebrafish research highlighted the critical role of cilia-driven cerebrospinal flow (CSF) in spine development, establishing CSF flow irregularities as an underlying biological cause of idiopathic scoliosis (IS). In this study, our objective was to assess and compare the cerebrospinal flow (CSF) of two populations (healthy adolescents and adolescents with IS) in order to detect anomalies similar to those in the animal model.
Methods
We conducted a prospective monocentric case-control study with 6 months of cohort follow-up. We enrolled 34 and 31 adolescents with and without IS, respectively. CSF flow was assessed using a 3T MRI scanner with axial phase-contrast flow sequences at the level of the mesencephalic aqueduct (MA), the 2nd cervical vertebra (C2), and the 7th thoracic vertebra (T7).
Results
Our results revealed no statistically significant difference in the CSF circulation characteristics (stroke volume, flow, velocity) at all three levels between the two groups. In the IS group, there was a positive correlation between the maximum CSF velocity at C2 and the Cobb angle (p = 0.014). There was also a positive correlation between the stroke volumes at the MA and C2 (p = 0.033), and between the CSF stroke volumes at C2 and T7 (p = 0.0012).
Conclusion
Our results revealed no difference in CSF flow characteristics between healthy and IS subjects. Further investigations would be pertinent to clarify the role of cerebrospinal fluid in correct spinal morphogenesis in humans.
A versatile recording device for the analysis of continuous daily external activity in colonies of highly eusocial bees
Authors: Justino Arthur Roque , Hartfelder Klaus 
Published: April 2024

Abstract

As pollinators, bees are key to maintaining the biodiversity of angiosperm plants, and for agriculture they provide a billion-dollar ecosystem service. But they also compete for resources (nectar and pollen), especially the highly social bees that live in perennial colonies. So, how do they organize their daily foraging activity? Here, we present a versatile, low-cost device for the continuous, automatic recording and data analysis of the locomotor activity in the colony-entrance tube of highly eusocial bees. Consisting of an in-house built block containing an infrared detector, the passage of bees in the colony entrance tunnel is registered and automatically recorded in an Arduino environment, together with concomitant recordings of temperature and relative humidity. With a focus on the highly diverse Neotropical stingless bees (Meliponini), we obtained 10-day consecutive recordings for two colonies each of the species Melipona quadrifasciata and Frieseomelitta varia, and also for the honey bee. The data were converted into CSV files, followed by the generation of actograms and Lomb-Scargle periodograms. We found a predominant circadian rhythmicity for all three species, but also indications of ultradian rhythms. For M. quadrifasciata, which is comparable in size to the honey bee, we found evidence for an anticipatory activity already before sunrise, followed by an early morning peak of activity. The cost and versatility of the device and the open-source options for data analysis make this an attractive system for conducting studies on circadian rhythms in social bees under natural conditions, complementing studies on flower visits by these important pollinators.
How to Achieve Government-business Collaboration in Information Technology Industry Policy? From the Perspective on Cost of Equity Capital
Authors: Huang Qiqing , Zou Ying , Zhao Yaxuan 
Published: April 2024

Abstract
This paper examines the impact and the mechanism of the information technology industry policy on the cost of equity capital from the perspective of the government-business collaboration based on the externality theory and soft budget constraint theory. This research selected the Chinese A-share non-financial listed companies from 2012 to 2019 as examples and empirically tests the cost of equity capital effect of the implementation of "Broadband China" policy based on the difference-in-difference (DID) model. The main test shows that the "Broadband China" policy has significantly reduced the cost of equity capital. The mechanism test reveals the function of "Broadband China" policy in optimizing the return, the configuration and the distribution of factors, and reflecting the government-business collaboration effect. Furthermore, the regional economic development, the industry competitiveness and the supply chain conflicts are proved to strengthen the above mechanisms respectively. The heterogeneity analysis shows that the effect of the "Broadband China" policy on the cost of equity capital is realized through the equity investment, positively affected by government-business relationship, and shows spatial aggregation effect. Actually, the digital transformation of the economy, led by the new generation of information technology, now provides the government and companies with opportunities for coordinated development. Therefore, for government, we recommend it to improve the support systems related to the information technology industry and give additional impetus to the development of the digital economy by using the benevolent regulatory measures. For companies, we also expect them to take advantage of the digital economy to achieve sustainable development.
JEL Classification: G12；G32；G38
Effectiveness of Amikacin Liposome Inhalation Suspension for Refractory Mycobacterium avium Complex Pulmonary Disease at 6 months post initiation
Authors: Urabe Naohisa , Sakamoto Susumu , Tokita Nozomi , Yoshida Hiromichi , Usui Yusuke , Shimizu Hiroshige , Sekiya Muneyuki , Miyoshi Shion , Nakamura Yasuhiko , Isobe Kazutoshi , Kishi Kazuma 
Published: April 2024

Abstract

Background
Amikacin liposome inhalation suspension (ALIS) improved sputum culture conversion rate at 6 months for patients with refractory Mycobacterium avium complex pulmonary disease (MAC-PD) in an international phase 3 trial. Patient characteristics and chest high-resolution CT (HRCT) findings associated with ALIS effectiveness are poorly documented.
Objective
This study aimed to clarify ALIS effectiveness for refractory MAC-PD at 6 months, elucidating associated patient characteristics and chest CT findings.
Methods
We reviewed medical records of 12 patients with refractory MAC-PD for whom ALIS treatment was initiated at Toho University Omori Medical Center from November 2021 through September 2022. All patients demonstrated treatment persistence for at least 3 months. They were divided into culture conversion and non-conversion groups using sputum culture conversion status after 6-month ALIS treatment initiation. Clinical and radiological characteristics were compared.
Results
Altogether, 7 of the 12 patients (58.3%) achieved sputum culture conversion within 6 months. The culture conversion group had shorter pre-ALIS initiation treatment duration [21 months (16–25) vs. 62 months (32–69); p = 0.045]; lower cavitary lesion incidence on HRCT (28.6% vs. 100%; p = 0.028); and fewer clarithromycin (CLA)-resistant strains [0/7 (0%) vs. 3/5 (60%); p = 0.045]. Chest HRCT findings improved in 2 of 7 (28.6%) and 2 of 5 (40%) patients in the culture conversion and non-conversion groups, respectively.
Conclusion
ALIS facilitated sputum culture conversion within 6 months in 58.3% of patients with refractory MAC-PD. Effectiveness was potentially higher for CLA-susceptible strains and patients with fewer cavitary lesions. Improved CT findings after ALIS did not always correspond to sputum culture conversion.
Anesthesia Depth Monitoring with Bispectral Index in Spinal Cord Stimulation Device Implantation for Chronic Disorders of Consciousness: A Retrospective Study
Authors: Chen Xuanling , Qin Xuewei , Zhuang Yutong , Li Zhengqian , Liang Zhenhu , Zhang Hua , Yao Lan , Li Xiaoli , He Jianghong , Guo Xiangyang 
Published: April 2024

Abstract

Objective: To assess whether maintaining an appropriate depth of anesthesia using the Bispectral Index (BIS) could enhance outcomes following spinal cord stimulation (SCS) device implantation in patients with chronic disorders of consciousness (DoC).
Methods: A total of 103 patients with DoC who underwent SCS implantation were reviewed between January 2019 and December 2021, of whom 83 met the inclusion and exclusion criteria. Patients were categorized into the BIS group (n = 45) and the non-BIS group (n = 38) based on whether BIS monitoring was used during the operation. Relevant data, such as disease course, cause, anesthesia, and operation time, were collected. Preoperative Coma Recovery Scale—Revised (CRS-R(preoperative)) score, postoperative CRS-R(24h), and postoperative CRS-R(3m) changes were recorded.
Results: The CRS-R(3m) score was significantly higher in the BIS group than that in the non-BIS group (preoperative), with a statistically significant difference (p < 0.05). Furthermore, in the CRS-R(24h), the BIS group demonstrated a higher score than the non-BIS group, with a statistically significant difference noted (X2 = 8.787, p = 0.004). When assessing the improvement in consciousness in the multivariate logistic regression analysis model, it was revealed that the thalamus independently influenced the enhancement of consciousness (p < 0.05). Throughout the follow-up, one patient in the BIS group experienced a decline in consciousness from a minimally consciousness state (MCS)- to-vegetative state, whereas two patients in the non-BIS group passed away during the follow-up.
Conclusion: For patients with DoC undergoing SCS implantation under general anesthesia, employing BIS monitoring during surgery to regulate the depth of sedation under general anesthesia can reduce propofol dosage and influence the brain network. Patients can benefit from improved hearing, as observed in the CRS-R(24h). We recommend using BIS to monitor the depth of anesthesia in patients with DoC to enhance overall patient outcomes.
Biochemical, Histopathological and Molecular Characterization of Myxobolus cerebralis Infection in Rainbow Trout (Oncorhynchus mykiss)
Authors: Zapryanova Dimitrinka , Urku Cigdem , Onalan Sukru , Tsandev Nikolay , Sandeva Gergana , Nikolov Galin 
Published: April 2024

Abstract
Myxobolus cerebralis, a myxosporean parasite, is the etiological agent of whirling disease in salmonids, which is responsible for high mortality and significant economic losses in rainbow trout (Oncorhynchus mykiss) populations. Тhis investigation was conducted to establish the blood biochemical changes caused by M. cerebralis in rainbow trout and fill the gaps from insufficient studies on the changes in blood parameters. Furthermore, the investigation aimed to identify the negative effect of M. cerebralis on some key physiological parameters to monitor its effects on the animal’s metabolism. The infection was confirmed by clinical, histopatological and qPCR screening. Histopathological changes were observed in the vertebrae, brain, gills, eyes, and muscles of the infected fish. The sporoplasm forms of the parasite were detected among the necrotic muscle cells. Mononuclear and eosinophilic cells were determined around ossifying and cartilage tissue of the vertebra; hyperplasia of the retina and cyst-like structures in the brain were also observed. Levels of total protein, globulins, cholesterol, alkaline phosphatase, and cortisol were greater than normal, while levels of creatinine, uric acid, albumin, calcium, and prolactin were lower than references for the species.
Association between Sarcopenia and the Risk of Depression: the mediating effects of Obesity
Authors: Li Bo , li Xiaotong , Guo Ruirui , Wang Sizhe , Wang Fengdan , Wu Zibo , Zhao Jing , Xin Sitong , Han Yu , Sun Mengzi 
Published: April 2024

Abstract

Background The increased incidence of depression in people with sarcopenia may further increase the disease burden in middle-aged and older adults. We aimed to explore whether obesity factors mediate the associations of sarcopenia with the risk of depression.Methods We included 5341 participants aged ≥ 45 years old in 2011–2012 and followed up until 2018. Sarcopenia consists of three factors as proxies, including muscle strength, appendicular skeletal muscle mass (ASM), and physical performance. The degree of obesity was reflected by BMI and WC. Cox proportional hazards regression models were used to examine the associations between sarcopenia, obesity and the risk of depression. The mediation effects of obesity indicators on the association between sarcopenia and depression were analyzed.Results Compared with no-sarcopenic group, the sarcopenic group had a higher risk of depression (HR, 1.238; 95%CI, 1.091–1.403). Only the interaction between gender and sarcopenia on depression was found in all subgroups. The results showed a significant association between sarcopenia and the risk of depression in the male population (HR, 1.224; 95%CI, 1.007–1.488). The association between sarcopenia and depression was partially mediated by BMI (mediated proportion = 31.81%) and WC (proportional mediation = 20.28%) in the Chinese middle-aged and elderly male population.Conclusion The Chinese middle-aged and elderly male population with sarcopenia had a higher risk of depression, and obesity partially mediated this association. Therefore, we suggested that appropriate weight gain in middle-aged and elderly male population might serve as an intervention to reduce future incidence of depression.