bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wellness motivation in cardiac rehabilitation: The role of self‐knowledge in cardiovascular risk modification
Authors: Fleury Julie , Sedikides Constantine 
Published: July 2007

AbstractUnderstanding the factors that motivate behavioral change is central to health promotion efforts. We used qualitative descriptive methods in an effort to understand the role of self‐knowledge in the process of risk factor modification. The sample consisted of 17 men and 7 women with diagnosed coronary heart disease, who were attempting to initiate and sustain programs of cardiovascular risk modification. Participants described self‐knowledge in terms of three contextually situated patterns: representational, evaluative, and behavioral action. Results reinforce the motivational role of the self and highlight the importance of understanding dimensions of self‐knowledge relevant to cardiovascular risk reduction. © 2007 Wiley Periodicals, Inc. Res Nurs Health 30:373–384, 2007.
High‐performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill
Authors: Rodrigues Clenilson M. , Rinaldo Daniel , Sannomiya Miriam , Santos Lourdes C. dos , Montoro Paola , Piacente Sonia , Pizza Cosimo , Vilegas Wagner 
Published: July 2007

AbstractThe isolation of polyphenolic compounds from an infusion of the Brazilian plant Davilla elliptica (Dilleniaceae), used as tea by virtue of its digestive properties, is described. An improved preparative HPLC method was used in order to isolate pure polyphenols from the complex mixture. Liquid–liquid extraction and solid‐phase extraction were employed to minimise the interference of polymeric compounds and to provide an enriched fraction of the compounds of interest. The identification of the isolated compounds was performed using analytical HPLC as well as direct injection electrospray ionisation ion trap tandem mass spectrometry (ESI‐IT‐MS/MS). The high flavonoid content suggests that D. elliptica may be a promising source of compounds to produce natural phytomedicines. Copyright © 2007 John Wiley & Sons, Ltd.
Preparative separation of four triterpene saponins from radix astragali by high‐speed counter‐current chromatography coupled with evaporative light scattering detection
Authors: Peng Jinyong , Dong Fuqiu , Qi Yan , Han Xu , Xu Youwei , Xu Lina , Xu Qiwei , Liu Kexin , Zhu Zhenyu 
Published: July 2007

AbstractFour triterpene saponins, including astragaloside IV, astragaloside II, astragaloside I and acetylastragaloside I, were successfully isolated and separated by high‐speed counter‐current chromatography coupled with evaporative light scattering detection from Radix Astragali using stepwise elution with a pair of solvent systems composed of n‐hexane:ethyl acetate:ethanol:water in volume ratios of 1:0.6:0.6:1 and 1:1:1:1 (by volume). The isolation produced 26.5 mg astragaloside IV, 28.2 mg astragaloside II, 48.7 mg astragaloside I and 17.6 mg acetylastragaloside I with purities of 97.6, 96.4, 98.8 and 96.8%, respectively, determined by high‐performance liquid chromatography from 250 mg crude extract. The chemical structures of the isolated compounds were identified by UV, NMR and MS, and confirmed by authentic standards. Copyright © 2007 John Wiley & Sons, Ltd.
In‐gel detection of urease activity by nitroprusside–thiol reaction
Authors: Sharma Vineet , Chaudhary Rajesh , Khurana J. M. , Muralidhar K. 
Published: July 2007

AbstractA new staining method for urease activity in non‐denaturing polyacrylamide gels is described. The increase in local pH of the gel, resulting from ureolytic activity of urease, causes a purple red coloured band after incubation of the polyacrylamide gel with urea. Staining of urease activity using this method is very specific for catalytically active urease even in crude preparations. Detection of urease activity by this method is rapid, simple and economical. The described method is also more sensitive than existing methods of urease staining. A minimum of 0.25 mU of urease activity can be detected after 5 min of incubation with the substrate. The method has been used to demonstrate the presence of different charge isoforms of urease in a member of the plant family Cucurbitaceae. Copyright © 2007 John Wiley & Sons, Ltd.
Simultaneous quantification of five triterpenoid saponins in Clematis L. Spp. by high‐performance liquid chromatography with evaporative light scattering detection
Authors: Sun Feng , He Qing , Xiao Peigen , Muhammad Ishtiaq , Cheng Yiyu 
Published: July 2007

AbstractA simple and accurate method involving high‐performance liquid chromatography with evaporative light scattering detection was developed for the simultaneous determination of five triterpenoid saponin components in Clematis L. spp. for the first time. The analysis was performed on a Zorbax SB‐C18 column and gradient elution with acetonitrile and water with 0.1% formic acid was utilised. All the calibration curves exhibited good linear characteristics with correlation coefficients in the range from 0.9979 to 0.9997. The limits of detection and limits of quantification were less than 0.152 and 0.506 µg, respectively. The overall recoveries for the five analytes were between 91.3 and 99.5%. A total of 10 samples from Clematis L. spp. were analysed under optimised conditions and the chemical profiles provided information for the identification of botanical origin, the development of new medicinal resources and chemotaxonomic investigation. Copyright © 2007 John Wiley & Sons, Ltd.
Primary flavonoids in marigold dye: extraction, structure and involvement in the dyeing process
Authors: Guinot Pauline , Gargadennec Annick , Valette Gilles , Fruchier Alain , Andary Claude 
Published: July 2007

AbstractFlavonoids extracted from marigold flowers were investigated for their dyeing potential. Patulitrin (1) and patuletin (2) were isolated and their structures established using NMR and HPLC‐MS. These compounds were identified as the main flavonoids present in the dyeing bath. Following the dyeing process, it was demonstrated that aglycone 2 bound more strongly to wool fibres than its glucoside 1. Moreover, analysis focused on 1 and 2 dynamics during plant growth revealed that these components were only found in flowers during and after flowering. The influence of growing location was also investigated and it appeared that cultivation under Mediterranean conditions enhanced biosynthesis of 1 and 2 . Finally, several solvents were tested for their potential to extract the flavonoids: the use of a water–ethanol mixture gave a high extraction efficiency and allowed selective extraction of 1 and 2. The implications of these results are discussed in relation to the development of marigold as a potential dyeing plant. Copyright © 2007 John Wiley & Sons, Ltd.
Proteome of mesenchymal stem cells
Authors: Park Hye Won , Shin Jun‐Seop , Kim Chan‐Wha 
Published: July 2007

AbstractProteomics has evolved, in recent years, into effective tools for basic and applied stem cell research, and has been extensively used to facilitate the identification of changes in signal transduction components, especially with regard to plasticity, proliferation, and differentiation. Several recent reports have also employed proteomic strategies to characterize human mesenchymal stem cells (hMSC) and their differentiated derivatives. Although these approaches have yielded valuable data, the results highlight the fact that only the limited numbers of proteins are characterized at the protein level in these cells, thus necessitating expandable MSC proteome dataset. This review presents, for the first time, an expandable list of MSC proteins, which will function as a starting point for the generation of a comprehensive reference map of their proteome. Also, the better way to bridge current gap between genomics and proteomics study such as integrated proteomic and transcriptomic analyses is discussed.
Mammalian plasma membrane proteomics
Authors: Josic Djuro , Clifton James G. 
Published: July 2007

AbstractPlasma membrane proteins serve essential functions for cells, interacting with both cellular and extracellular components, structures and signaling molecules. Additionally, plasma membrane proteins comprise more than two‐thirds of the known protein targets for existing drugs. Consequently, defining membrane proteomes is crucial to understanding the role of plasma membranes in fundamental biological processes and for finding new targets for action in drug development. MS‐based identification methods combined with chromatographic and traditional cell‐biology techniques are powerful tools for proteomic mapping of proteins from organelles. However, the separation and identification of plasma membrane proteins remains a challenge for proteomic technology because of their hydrophobicity and microheterogeneity. Creative approaches to solve these problems and potential pitfalls will be discussed. Finally, a representative overview of the impressive achievements in this field will also be given.
Public proteomic MS repositories and pipelines: available tools and biological applications
Authors: Mead Jennifer A. , Shadforth Ian P. , Bessant Conrad 
Published: July 2007

AbstractAs proteomic MS has increased in throughput, so has the demand to catalogue the increasing number of peptides and proteins observed by the community using this technique. As in other ‘omics’ fields, this brings obvious scientific benefits such as sharing of results and prevention of unnecessary repetition, but also provides technical insights, such as the ability to compare proteome coverage between different laboratories, or between different proteomic platforms. Journals are also moving towards mandating that proteomics data be submitted to public repositories upon publication. In response to these demands, several web‐based repositories have been established to store protein and peptide identifications derived from MS data, and a similar number of peptide identification software pipelines have emerged to deliver identifications to these repositories. This paper reviews the latest developments in public domain peptide and protein identification databases and describes the analysis pipelines that feed them. Recent applications of the tools to pertinent biological problems are examined, and through comparing and contrasting the capabilities of each system, the issues facing research users of web‐based repositories are explored. Future developments and mechanisms to enhance system functionality and user‐interfacing opportunities are also suggested.
Proteoglycanomics: tools to unravel the biological function of glycosaminoglycans
Authors: Gesslbauer Bernd , Rek Angelika , Falsone Fabio , Rajkovic Erich , Kungl Andreas J. 
Published: July 2007

AbstractGlycosylation is the most frequent PTM and contributes significantly to the function of proteins depending on the type of glycosylation. Especially glycan structures like the glycosaminoglycans are considered to constitute themselves the major function of the glycoconjugate which is therefore termed proteoglycan. Here we review recent views on and novel tools for analysing the proteoglycanome, which are directly related to the type of glycanation under investigation. We define the major function of the proteoglycanome to be its interaction with various proteins in many different (patho‐)physiological conditions. This is exemplified by the differential glycosaminoglycan‐interactome of healthy versus arthritic patient sera.
Bayesian methods for proteomics
Authors: Alterovitz Gil , Liu Jonathan , Afkhami Ehsan , Ramoni Marco F. 
Published: July 2007

AbstractBiological and medical data have been growing exponentially over the past several years [1, 2]. In particular, proteomics has seen automation dramatically change the rate at which data are generated [3]. Analysis that systemically incorporates prior information is becoming essential to making inferences about the myriad, complex data [4–6]. A Bayesian approach can help capture such information and incorporate it seamlessly through a rigorous, probabilistic framework. This paper starts with a review of the background mathematics behind the Bayesian methodology: from parameter estimation to Bayesian networks. The article then goes on to discuss how emerging Bayesian approaches have already been successfully applied to research across proteomics, a field for which Bayesian methods are particularly well suited [7–9]. After reviewing the literature on the subject of Bayesian methods in biological contexts, the article discusses some of the recent applications in proteomics and emerging directions in the field.
Transient absorption investigation of the photophysical properties of new photochromic 3H‐naphtho[2,1‐b]pyran
Authors: Moine B. , Buntinx G. , Poizat O. , Rehault J. , Moustrou C. , Samat A. 
Published: July 2007

AbstractThe photophysical and photochemical properties of two 3,3‐diphenyl‐3H‐naphtho[2,1‐b]pyrans substituted in position 8 by phenylethynyl and p‐nitrophenylethynyl groups, respectively, are investigated by femtosecond and nanosecond transient absorption spectroscopy. The spectral characteristics and dynamics of the lowest excited singlet and triplet states are determined. The ring‐opening reaction responsible for the photochromism is observed to occur in the S1 state. Copyright © 2007 John Wiley & Sons, Ltd.
Characterization of electron beam irradiation blends based on metallocene ethylene‐1‐octene copolymer
Authors: Cárdenas Miguel Angel , Villarreal Norky , Gobernado‐Mitre Isabel , Merino Juan Carlos , Pastor José María 
Published: July 2007

AbstractBinary blends using metallocene ethylene‐1‐octene copolymer as matrix were prepared and subjected to electron beam (EB) irradiation (50, 100, and 200 kGy). Gel content and melt flow index values indicated that the blends were crosslinking. Fourier transform infrared‐ATR spectroscopy was used to study the crosslinking and oxidative degradation of the blends via tertiary carbon and carboxyl group formation, respectively. Thermal and mechanical properties were studied showing that the crystallinity of both matrix and dispersed phase decreased with irradiation dose, and that the thermoplastic elastomers with good mechanical properties may be obtained by EB irradiation. Chain branching and scission were also detected at all irradiation doses, although at the highest doses (200 kGy) a crosslinking reaction was the most predominantly observed effect. The successive self‐nucleation annealing technique was used to determine the EB irradiation effects on crystallization of some blends in which crosslinking and chain branching take place, modifying the chain's structure and therefore crystalline regions in the matrix and the dispersed phase. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 2432–2440, 2007
High melting thermoplastic/epoxy resin blends: Influence of the curing reaction on the crystallization and melting behavior
Authors: Goossens S. , Groeninckx G. 
Published: July 2007

AbstractThe influence of the cure process and the resulting reaction‐induced phase separation (RIPS) on the crystallization and melting behavior of polyoxymethylene (POM) in epoxy resin diglycidylether of bisphenol A (DGEBA) blends has been studied at different cure temperatures (180 and 145 °C). The crystallization and melting behavior of POM was studied with DSC and the simultaneous blend morphology changes were studied using OM. At first, the influence of the epoxy monomer on the dynamically crystallized POM was investigated. Secondly, a cure temperature above the melting point of POM (Tcure = 180 °C) was applied for blends with curing agent to study the influence of resulting phase morphology types on the crystallization behavior of POM in the epoxy blends. Large differences between particle/matrix and phase‐inverted structures have been observed. Thirdly, the cure temperature was lowered below the melting temperature of POM, inducing isothermal crystallization prior to RIPS. As a consequence, a distinction was made between dynamically and isothermally crystallized POM. Concerning the dynamically crystallized material, a clear difference could be made between the material crystallized in the homogeneous sample and that crystallized in the phase‐separated structures. The isothermally crystallized POM was to a large extent influenced by the conversion degree of the epoxy resin. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 2456–2469, 2007
Clinical correlations and pulmonary function at 8 years of age after severe neonatal respiratory failure
Authors: Majaesic Carina M. , Jones Richard , Dinu Irina A. , Montgomery Mark D. , Sauve Reginald S. , Robertson Charlene M.T. 
Published: July 2007

AbstractObjectives:The aim of this study was to determine the pulmonary sequelae of severe neonatal respiratory failure.Study Design:This was a multicenter, prospective study. Fifty‐four survivors of neonatal respiratory failure (oxygenation indices >25 on two occasions), completed pulmonary function testing at 8 years of age. Thirty‐one (57%) received extracorporeal membrane oxygenation (ECMO). Pulmonary outcome was based on spirometry and lung volume data. Pulmonary outcome for each diagnostic and treatment group is reported as mean and as percent predicted. Individually subjects were also classified based on spirometry, as either normal, obstructed (defined as forced expiratory volume (FEV1) in 1 sec:forced vital capacity (FVC) of <80 % predicted, or with reduced FVC (FCV of <80% predicted) with normal FEV1/FVC. Risk for adverse outcome was determined using univariate analysis.Results:Mean FVC, FEV1 and FEV25–75 were reduced in the total cohort. The reduction was greatest in the subgroup with CDH and the group treated with ECMO. Assessed individually, 54% of subjects had normal spirometry and lung volumes, 19% airflow obstruction, and 27% reduced FVC. Poorer pulmonary outcome was linked to ECMO, congenital diaphragmatic hernia (CDH), birth weight for gestational age <10th percentile, duration of hospitalization, or need for prolonged supplemental oxygen.Conclusion:Neonates with severe respiratory failure due to CDH or needing ECMO and small for gestation are at increased risk of poorer pulmonary outcome and require close follow‐up. Pediatr Pulmonol. 2007; 42:829–837. © 2007 Wiley‐Liss, Inc.
Human kallikrein‐related peptidase 12: Antibody generation and immunohistochemical localization in prostatic tissues
Authors: Memari Nader , Diamandis Eleftherios P. , Earle Tammy , Campbell Alison , Van Dekken Herman , Van der Kwast Theodore H. 
Published: July 2007

AbstractBackgroundHuman tissue kallikrein‐related peptidases (genes, KLKs; proteins, KLKs) are a subgroup of serine proteases present in a variety of tissues and biological fluids. A number of human tissue KLKs are established or candidate serologic biomarkers for prostate cancer. Human kallikrein‐related peptidase 12 (KLK12, KLK12), recently identified in our laboratory, is a novel member of the KLK gene family. Here, we report generation of antibodies against the full‐length recombinant KLK12 (classical form) and the immunohistological localization of this KLK in normal and malignant prostate tissues.MethodsThe mature form of KLK12 cDNA was amplified using PCR and cloned into a plasmid vector for protein production in E. coli. Following identification by mass spectroscopy, recombinant KLK12 was purified and used as immunogen in rabbits. Anti‐ KLK12 antibody was used for immunostaining of paraffin‐embedded sections of human prostate tissue. Immunoexpression of KLK12 in benign and malignant prostate tissue was evaluated using a prostate cancer tissue array.ResultsAnti‐KLK12 antibody showed a predominantly apical and membranous staining of the luminal cells of the normal prostate in contrast with the predominantly diffuse cytoplasmic staining observed in both prostatic intra‐epithelial neoplasia and adenocarcinomas. This was occasionally associated with an intense granular supranuclear staining. More than 95% of the prostate cancers on the tissue microarray were KLK12 positive.ConclusionHigher levels of KLK12 in malignant prostatic glands, and the shift in subcellular localization of KLK12 in prostate cancer observed in this study point to the potential role of this kallikrein during prostate carcinogenesis. Prostate 67: 1465–1474, 2007. © 2007 Wiley‐Liss, Inc.
Statistical recombinant mapping in extended high‐risk Utah pedigrees narrows the 8q24 prostate cancer locus to 2.0 Mb
Authors: Camp Nicola J. , Farnham James M. , Allen‐Brady Kristina , Cannon‐Albright Lisa A. 
Published: July 2007

AbstractBackgroundChromosome 8q24 is a region of compelling interest for prostate cancer (PRCA). Linkage, association, and admixture analysis initially indicated the region. Subsequently, several variants at 8q24 have been found to independently associate with PRCA. One compelling hypothesis is allelic heterogeneity, whereby multiple variants affect the regulation of the same gene. In addition to potential allelic heterogeneity, 8q24 exhibits strong linkage disequilibrium over vast distances and is prone to chromosomal aberrations.MethodsAllelic heterogeneity and complex chromosomal structure are factors that hinder progress towards gene identification using association. Linkage techniques, however, are robust to allelic heterogeneity and therefore can contribute important localization information. We have used a linkage‐based localization technique to perform recombinant mapping in eight high‐risk Utah pedigrees.ResultsUsing pedigree‐based recombinant mapping we identify a 5.7 Mb region at 8q24.21–22, between markers D8S1774 and D8S557. Considering our region with the localization previously presented, we can delimit the 8q24 PRCA locus to a 2.0 Mb consensus interval from 127.5 to 129.5 Mb.ConclusionWe have used a linkage‐based, recombinant mapping technique in extended high‐risk Utah pedigrees to successfully narrow the 8q24 PRCA locus. The region we present halves the 95% credible interval previously described using admixture analysis and contains only three annotated genes: FAM84B, MYC, and TMEM75. It should be noted that LD stretches far beyond the interval we describe, and hence this region adds important information about where to focus future efforts to isolate the causal variants and underlying gene, whether by linkage or association techniques. Prostate 67: 1456–1464, 2007. © 2007 Wiley‐Liss, Inc.