bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case Study of Severe Winter Convection in the Midwest
Authors: Pettegrew Brian P. , Market Patrick S. , Wolf Raymond A. , Holle Ronald L. , Demetriades Nicholas W. S. 
Published: May 2008

AbstractBetween 2100 UTC 11 February 2003 and 0200 UTC 12 February 2003, a line of thunderstorms passed swiftly through parts of eastern Iowa and into north-central Illinois. Although this storm somewhat resembled a warm season, line-type mesoscale convective system, it was unique in that the thunderstorm winds exceeded the severe criterion (50 kt; 25.7 m s−1) during a snowburst. While the parent snowband deposited only 4 cm of snow, it did so in a short period and created a treacherous driving situation because of the ensuing near-whiteout conditions caused by strong winds that reached the National Weather Service severe criteria, as the line moved across central Illinois. Such storms in the cold season rarely occur and are largely undocumented; the present work seeks to fill this void in the existing literature.While this system superficially resembled a more traditional warm season squall line, deeper inspection revealed a precipitation band that failed to conform to that paradigm. Radar analysis failed to resolve any of the necessary warm season signatures, as maximum reflectivities of only 40–45 dBZ reached no higher than 3.7 km above ground level. The result was low-topped convection in a highly sheared environment. Moreover, winds in excess of 50 kt (25.7 m s−1) occurred earlier in the day without thunderstorm activity, upstream of the eventual severe thundersnow location. Perhaps of greatest importance is the fact that the winds in excess of the severe criterion were more the result of boundary layer mixing, and largely coincident with the parent convective line. This event was a case of forced convection, dynamically linked to its parent cold front via persistent frontogenesis and the convective instability associated with it; winds sufficient for a severe thunderstorm warning, while influenced by convection, resulted from high momentum mixing downward through a dry-adiabatic layer.
Resistance to second-line injectables and treatment outcomes in multidrug-resistant and extensively drug-resistant tuberculosis cases
Authors: Migliori G. B. , Lange C. , Centis R. , Sotgiu G. , Mütterlein R. , Hoffmann H. , Kliiman K. , De Iaco G. , Lauria F. N. , Richardson M. D. , Spanevello A. , Cirillo D. M. , TBNET Study Group  
Published: May 2008

No information is currently available on the influence of injectable second-line drugs on treatment outcomes of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) patients.To investigate this issue, a large series of MDR- and XDR-TB cases diagnosed in Estonia, Germany, Italy and the Russian Federation (Archangels Oblast) between 1999 and 2006 were analysed. All study sites performed drug susceptibility testing for first- and second-line anti-TB drugs, laboratory quality assurance and treatment delivery according to World Health Organization recommendations.Out of 4,583 culture-confirmed cases, 240 MDR- and 48 XDR-TB cases had a definitive outcome recorded (treatment success, death, failure). Among MDR- and XDR-TB cases, capreomycin resistance yielded a higher proportion of failure and death than capreomycin-susceptible cases. Resistance to capreomycin was independently associated with unfavourable outcome (logistic regression analysis: odds ratio 3.51).In the treatment of patients with multidrug-resistant and extensively drug-resistant tuberculosis, resistance to the injectable drug capreomycin was an independent predictor for therapy failure in this cohort. AsMycobacterium tuberculosisdrug resistance is increasing worldwide, there is an urgent need for novel interventions in the fight against tuberculosis.
Risk factors for death among tuberculosis cases: analysis of European surveillance data
Authors: Lefebvre N. , Falzon D. 
Published: May 2008

The proportion of tuberculosis (TB) patients in the European Union (EU) who die remains high (8% overall). The aim of the present study was to quantify the risk of dying associated with demographic and clinical factors.Case-based data on 39,566 TB patients notified by 15 EU countries during 2002–2004 were analysed using logistic regression.It was observed that advancing age and resistance to isoniazid and rifampicin were the strongest determinants of death, while male sex, European origin, pulmonary site of disease and previous anti-TB treatment were weaker predictors. Risk varied between reporting countries, presumably reflecting differences in patient profiles, reporting practices and programme effectiveness.In conclusion, earlier suspicion, diagnosis and treatment may reduce deaths, particularly among the elderly. Special attention is needed to avert the development and transmission of multidrug-resistant tuberculosis.
Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma
Authors: Huang Qin , Yu Chenggong , Zhang Xiaoqi , Goyal Raj K 
Published: May 2008

Abstract

Background
The purpose of this study was to compare DNA histograms obtained by standard flow cytometry (FC) and high fidelity image cytometry on sections (ICS) in normal gastrointestinal mucosa and Barrett's adenocarcinoma (BAC).

Methods
Archival formalin-fixed paraffin-embedded tissue blocks of 10 normal controls from 10 subjects and 42 BAC tissues from 17 patients were examined. DNA FC was performed using standard techniques and ICS was carried out by Automated Cellular Imaging System (ACIS). DNA ploidy histograms were classified into diploid with peak DNA index (DI) at 0.9–1.1, and aneuploid with peak DI > 1.1. DI values of aneuploid peaks were determined. Additionally, for DNA ICS, heterogeneity index (HI) representing DNA content heterogeneity, and histograms containing cells with DI > G2 were also identified.

Results
All control samples were diploid by both FC and ICS analyses. In BAC, FC showed diploid peaks in 29%, diploid peaks with additional aneuploid or tetraploid peaks in 57%, and 14% of the samples, respectively. In contrast, ICS showed aneuploid peaks in all the cases with peak DI > 1.25; 37 cases had peak DI between 1.25 and 2.25; and 5 cases had peak DI > 2.25. HI values (mean ± SD) were 11.3 ± 1.1 in controls and 32.4 ± 8.5 in BAC (p < 0.05). Controls had no G2 exceeding cells. However, 19/37 (51%) of the cases with primary peak DI < 2.25 had cells exceeding 9N.

Conclusion
ICS detects DNA aneuploidy in all BAC samples while FC missed the diagnosis of aneuploidy in 29%. In addition, ICS provides more information on HI and G2 exceeding rates.
Exposure from the Chernobyl accident had adverse effects on erythrocytes, leukocytes, and, platelets in children in the Narodichesky region, Ukraine: A 6-year follow-up study
Authors: Stepanova Eugenia , Karmaus Wilfried , Naboka Marina , Vdovenko Vitaliy , Mousseau Tim , Shestopalov Viacheslav M , Vena John , Svendsen Erik , Underhill Dwight , Pastides Harris 
Published: May 2008

AbstractBackgroundAfter the Chernobyl nuclear accident on April 26, 1986, all children in the contaminated territory of the Narodichesky region, Zhitomir Oblast, Ukraine, were obliged to participate in a yearly medical examination. We present the results from these examinations for the years 1993 to 1998. Since the hematopoietic system is an important target, we investigated the association between residential soil density of137Caesium (137Cs) and hemoglobin concentration, and erythrocyte, platelet, and leukocyte counts in 1,251 children, using 4,989 repeated measurements taken from 1993 to 1998.MethodsSoil contamination measurements from 38 settlements were used as exposures. Blood counts were conducted using the same auto-analyzer in all investigations for all years. We used linear mixed models to compensate for the repeated measurements of each child over the six year period. We estimated the adjusted means for all markers, controlling for potential confounders.ResultsData show a statistically significant reduction in red and white blood cell counts, platelet counts and hemoglobin with increasing residential137Cs soil contamination. Over the six-year observation period, hematologic markers did improve. In children with the higher exposure who were born before the accident, this improvement was more pronounced for platelet counts, and less for red blood cells and hemoglobin. There was no exposure×time interaction for white blood cell counts and not in 702 children who were born after the accident. The initial exposure gradient persisted in this sub-sample of children.ConclusionThe study is the first longitudinal analysis from a large cohort of children after the Chernobyl accident. The findings suggest persistent adverse hematological effects associated with residential137Cs exposure.
Novel PKC signaling is required for LPS-induced soluble Flt-1 expression in macrophages
Authors: Lee Ming-Cheng , Wei Shu-Chen , Tsai-Wu Jyy-Jih , Wu C H Herbert , Tsao Po-Nien 
Published: May 2008

AbstractIn vitro activation of macrophages by LPS induces rapid release of vascular endothelial growth factor (VEGF) and soluble fms-like tyrosine kinase-1 receptor (sFlt-1), which are thought to be the effectors to cause sepsis. However, the signal pathway that controls the VEGF and sFlt-1 expressions in LPS-activated macrophages remains unclear. In this study, we demonstrated that phosphorylation of protein kinase C (PKC)δ played a key role in the VEGF and sFlt-1 signaling pathway of LPS-activated macrophages. PKC is a family of serine-threonine kinases, which are classified into three major groups based on homology and cofactor requirements: conventional PKCs, novel PKCs, and atypical PKCs. In the murine RAW264.7 cells, as well as in primary human monocytes/macrophages, pretreatment with a general PKC inhibitor GF109203X or with a novel PKCδ inhibitor rottlerin or overexpression of a kinase-inactive form of PKCδ (K376R) eliminated LPS-induced sFlt-1 expression and augmented LPS-induced VEGF expression at the protein and the transcription levels. In contrast, Gö6976, an inhibitor for the conventional PKCs, or myristoylated PKCζ pseudosubstrate peptide, an inhibitor for the atypical PKCs, failed to exert the same effects. These data suggest that PKCδ signaling is involved in LPS-induced sFlt-1 expression and serves as a negative mediator in LPS-induced VEGF expression in macrophages. A novel strategy controlling the LPS-induced PKC pathways, especially the PKCδ isoform, may be developed based on this study.
Aquaporin 1 Is Important for Maintaining Secretory Granule Biogenesis in Endocrine Cells
Authors: Arnaoutova Irina , Cawley Niamh X. , Patel Nimesh , Kim Taeyoon , Rathod Trushar , Loh Y. Peng 
Published: May 2008

AbstractAquaporins (AQPs), a family of water channels expressed in epithelial cells, function to transport water in a bidirectional manner to facilitate transepithelial fluid absorption and secretion. Additionally, AQP1 and AQP5 are found in pancreatic zymogen granules and synaptic vesicles and are involved in vesicle swelling and exocytosis in exocrine cells and neurons. Here, we show AQP1 is in dense-core secretory granule (DCSG) membranes of endocrine tissue: pituitary and adrenal medulla. The need for AQP1 in endocrine cell function was examined by stable transfection of AQP1 antisense RNA into AtT20 cells, a pituitary cell line, to down-regulate AQP1 expression. These AQP1-deficient cells showed more than 60% depletion of DCSGs and significantly decreased DCSG protein levels, including proopiomelanocotin/pro-ATCH and prohormone convertase 1/3, but not non-DCSG proteins. Pulse-chase studies revealed that whereas DCSG protein synthesis was unaffected, approximately 50% of the newly synthesized proopiomelanocortin was degraded within 1 h. Low levels of ACTH were released upon stimulation, indicating that the small number of DCSGs that were made in the presence of the residual AQP1 were functionally competent for exocytosis. Analysis of anterior pituitaries from AQP1 knockout mice showed reduced prohormone convertase 1/3, carboxypeptidase E, and ACTH levels compared to wild-type mice demonstrating that our results observed in AtT20 cells can be extended to the animal model. Thus, AQP1 is important for maintaining DCSG biogenesis and normal levels of hormone secretion in pituitary endocrine cells.
The Xenopus laevis Isoform of G Protein-Coupled Receptor 3 (GPR3) Is a Constitutively Active Cell Surface Receptor that Participates in Maintaining Meiotic Arrest in X. laevis Oocytes
Authors: Deng James , Lang Stephanie , Wylie Christopher , Hammes Stephen R. 
Published: May 2008

Abstract
Oocytes are held in meiotic arrest in prophase I until ovulation, when gonadotropins trigger a subpopulation of oocytes to resume meiosis in a process termed “maturation.” Meiotic arrest is maintained through a mechanism whereby constitutive cAMP production exceeds phosphodiesterase-mediated degradation, leading to elevated intracellular cAMP. Studies have implicated a constitutively activated Gαs-coupled receptor, G protein-coupled receptor 3 (GPR3), as one of the molecules responsible for maintaining meiotic arrest in mouse oocytes. Here we characterized the signaling and functional properties of GPR3 using the more amenable model system of Xenopus laevis oocytes. We cloned the X. laevis isoform of GPR3 (XGPR3) from oocytes and showed that overexpressed XGPR3 elevated intraoocyte cAMP, in large part via Gβγ signaling. Overexpressed XGPR3 suppressed steroid-triggered kinase activation and maturation of isolated oocytes, as well as gonadotropin-induced maturation of follicle-enclosed oocytes. In contrast, depletion of XGPR3 using antisense oligodeoxynucleotides reduced intracellular cAMP levels and enhanced steroid- and gonadotropin-mediated oocyte maturation. Interestingly, collagenase treatment of Xenopus oocytes cleaved and inactivated cell surface XGPR3, which enhanced steroid-triggered oocyte maturation and activation of MAPK. In addition, human chorionic gonadotropin-treatment of follicle-enclosed oocytes triggered metalloproteinase-mediated cleavage of XGPR3 at the oocyte cell surface. Together, these results suggest that GPR3 moderates the oocyte response to maturation-promoting signals, and that gonadotropin-mediated activation of metalloproteinases may play a partial role in sensitizing oocytes for maturation by inactivating constitutive GPR3 signaling.
Mitochondria in energy-limited states: mechanisms that blunt the signaling of cell death
Authors: Hand Steven C. , Menze Michael A. 
Published: May 2008

SUMMARYCellular conditions experienced during energy-limited states –elevated calcium, shifts in cellular adenylate status, compromised mitochondrial membrane potential – are precisely those that trigger, at least in mammals, the mitochondrion to initiate opening of the permeability transition pore, to assemble additional protein release channels, and to release pro-apoptotic factors. These pro-apototic factors in turn activate initiator and executer caspases. How is activation of mitochondria-based pathways for the signaling of apoptotic and necrotic cell death avoided under conditions of hypoxia, anoxia, diapause, estivation and anhydrobiosis?Functional trade-offs in environmental tolerance may have occurred in parallel with the evolution of diversified pathways for the signaling of cell death in eukaryotic organisms. Embryos of the brine shrimp, Artemia franciscana, survive extended periods of anoxia and diapause, and evidence indicates that opening of the mitochondrial permeability transition pore and release of cytochrome c (cyt-c) do not occur. Further, caspase activation in this crustacean is not dependent on cyt-c. Its caspases display regulation by nucleotides that is consistent with `applying the brakes' to cell death during energy limitation. Unraveling the mechanisms by which organisms in extreme environments avoid cell death may suggest possible interventions during disease states and biostabilization of mammalian cells.
The effect of proline on the network structure of major ampullate silks as inferred from their mechanical and optical properties
Authors: Savage Ken N. , Gosline John M. 
Published: May 2008

SUMMARYThe silk that orb-weaving spiders produce for use as dragline and for the frame of the web is spun from the major ampullate (MA) glands, and it is renowned for its exceptional toughness. The fibroins that make up MA silk have previously been organized into two major groupings, spidroin-1 and spidroin-2,based largely on differences in amino acid sequence. The most apparent difference between spidroin-1 and spidroin-2 fibroins is the lack of proline in spidroin-1. The MA silk of Araneus diadematus comprises two spidroin-2 fibroins, and is therefore proline-rich, whereas spidroin-1 is preferentially expressed in Nephila clavipes MA silk, and so this silk is proline deficient. Together, these two silks provide a system for testing the consequences of proline-rich and proline-deficient fibroin networks. This study measures the mechanical and optical properties of dry and hydrated Araneus and Nephila MA silks. Since proline acts to disrupt secondary structure, it is hypothesized that the fibroin network of Araneus MA silk will contain less secondary structure than the network of Nephila MA silk. Mechanical and optical studies clearly support this hypothesis. Although the dry properties of these two silks are indistinguishable, there are large differences between the hydrated silks. Nephila silk does not swell upon hydration to the same degree as Araneus silk. In addition, upon hydration, Nephila MA silk retains more of its initial dry stiffness, and retains more molecular order,as indicated by birefringence measurements.
Motor unit recruitment patterns 1: responses to changes in locomotor velocity and incline
Authors: Hodson-Tole Emma F. , Wakeling James M. 
Published: May 2008

SUMMARYMammalian skeletal muscles are composed of a mixture of motor unit types,which contribute a range of mechanical and physiological properties to the muscle. For a muscle to effectively contribute to smooth, co-ordinated movement it must activate an appropriate number and combination of motor units to generate the required force over a suitable time period. Much evidence exists indicating that motor units are activated in an orderly fashion, from the slowest through to the fastest. A growing body of evidence, however,indicates that such a recruitment strategy does not always hold true. Here we investigate how motor unit recruitment patterns were influenced by changes in locomotor velocity and incline. Kinematics data and myoelectric signals were collected from three rat ankle extensor muscles during running on a treadmill at nine velocity and incline combinations. Wavelet and principal component analysis were used to simultaneously decompose the signals into time and frequency space. The relative frequency components of the signals were quantified during 20 time windows of a stride from each locomotor condition. Differences in signal frequency components existed between muscles and locomotor conditions. Faster locomotor velocities led to a relative increase in high frequency components, whereas greater inclines led to a relative increase in the low frequency components. These data were interpreted as representing changes in motor unit recruitment patterns in response to changes in the locomotor demand. Motor units were not always recruited in an orderly manner, indicating that recruitment is a multi-factorial phenomenon that is not yet fully understood.
Motor unit recruitment patterns 2: the influence of myoelectric intensity and muscle fascicle strain rate
Authors: Hodson-Tole Emma F. , Wakeling James M. 
Published: May 2008

SUMMARYTo effectively meet the force requirements of a given movement an appropriate number and combination of motor units must be recruited between and within muscles. Orderly recruitment of motor units has been shown to occur in a wide range of skeletal muscles, however, alternative strategies do occur. Faster motor units are better suited to developing force rapidly, and produce higher mechanical power with greater efficiency at faster shortening strain rates than slower motor units. As the frequency content of the myoelectric signal is related to the fibre type of the active motor units, we hypothesised that, in addition to an association between myoelectric frequency and intensity, there would be a significant association between muscle fascicle shortening strain rate and myoelectric frequency content. Myoelectric and sonomicrometric data were collected from the three ankle extensor muscles of the rat hind limb during walking and running. Myoelectric signals were analysed using wavelet transformation and principal component analysis to give a measure of the signal frequency content. Sonomicrometric signals were analysed to give measures of muscle fascicle strain and strain rate. The relationship between myoelectric frequency and both intensity and muscle fascicle strain rate was found to change across the time course of a stride,with differences also occurring in the strength of the associations between and within muscles. In addition to the orderly recruitment of motor units, a mechanical strategy of motor unit recruitment was therefore identified. Motor unit recruitment is therefore a multifactorial phenomenon, which is more complex than typically thought.
The cloning of eel osmotic stress transcription factor and the regulation of its expression in primary gill cell culture
Authors: Tse W. K. F. , Chow S. C. , Wong C. K. C. 
Published: May 2008

SUMMARY
In the present study, we aimed to clone an osmotic stress transcriptional factor (Ostf) from gill cells of Japanese eels. In addition, we measured its expression in Percoll™-gradient-isolated gill chloride (CC) and pavement(PVC) cells and determined the regulation of its expression in primary gill cell culture. Using degenerative primers and RACE techniques, we cloned a cDNA of 615bp, encompassing the coding sequence of Ostf (204 amino acids). The cloned Ostf1 DNA sequence shared 84% DNA homology with the Ostf1 of tilapia. In general, the basal Ostf expression level was found to be significantly higher in CCs than in PVCs. In the direct transfer of fish from freshwater to seawater, a significant but transient induction of Ostf mRNA in CCs and PVCs was measured after 6h of acclimation. Compared with gill CCs, the level of induction measured at PVCs was lower. In the seawater-to-freshwater transfer,no significant change in Ostf transcript levels was detected in either CCs or PVCs. To decipher the regulatory mechanism of Ostf expression, we conducted experiments using primary gill cell culture to specifically address the involvement of two putative osmosensors (i.e. intracellular ion strength/macromolecular crowding and cytoskeleton) in the regulation of Ostf expression. Hypertonic treatment using impermeable solutes (i.e. NaCl,500mOsmoll–1) induced Ostf mRNA expression in 6h, but no noticeable effect was measured using permeable solute (i.e. urea,500mOsmoll–1). The induction was transcriptionally regulated and was abolished by the addition of organic osmolytes (i.e. betaine, inositol or taurine) into the culture media. Addition of colchicine (an inhibitor of microtubule polymerization) to hypertonic (with added NaCl,500mOsmoll–1) cells reduced Ostf mRNA expression, suggesting that an increase in intracellular ionic strength and the integrity of the cytoskeleton are involved in the activation of Ostf mRNA expression in the cells. Collectively, the results of this study reveal, for the first time, the differential expression of Ostf in isolated CCs and PVCs. The resulting knowledge can shed light on how Ostf participates in hyperosmotic adaptation in fish gills.