bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimalisasi Self Efficacy Pasien Chronic Kidney Disease (CKD) melalui Edukasi Pembatasan Cairan
Authors: Akbar Akbar Asfar , Sudarman  , Wan Sulastri Emin  , Brajakson Siokal  , Wa Ode Sri Asnaniar  , Yusrah Taqyiah  , Fatma Jama  , Suhermi  , Samsualam  
Published: March 2024

Chronic kidney disease is a clinical condition characterized by a progressive and irreversible decline in kidney function, requiring appropriate renal replacement therapy in the form of kidney transplantation or dialysis. The prevalence of chronic kidney disease (CKD) is increasing, as is the case with chronic kidney failure patients undergoing hemodialysis therapy. Self-efficacy is a belief that determines how a person thinks, motivates himself and decides to perform a behavior to achieve the desired goal. Self efficacy affects the quality of life of CKD patients undergoing hemodialysis. Regulation of fluid intake is very important and can affect the increase in Interdialytic Waight Gain (IDWG).
Weight gain is a major problem in patients undergoing hemodialysis therapy of more than 4 kg. During interviews with patients undergoing therapy, it turned out that there were still many who had not done fluid restriction as recommended. The method used in community service activities is by lectures/counseling, discussion and question and answer. The results of the dedication showed that the education participants listened well and understood the importance of fluid restriction in patients with CKD. The results of this activity increase the patient's understanding or self-efficacy based on the results of pre-test and post-test evaluations regarding fluid restriction.
Keywords: Chronic Kidney Disease, Hemodialysis, Self Efficacy
Edukasi Masyarakat Dalam Meningkatkan Pengetahuan Tentang Stunting di Desa Lembang Maroson
Authors: Darlis Idhar , Elyanovianti  , Rahayu Yuli 
Published: March 2024

Salah satu masalah besar yang dihadapi dunia saat ini adalah kejadian stunting pada balita. WHO memprediksi sebesar 127 juta anak di bawah usia 5 tahun akan mengalami stunting pada tahun 2025. Stunting adalah kondisi ketika anak lebih pendek dibandingkan anak-anak lain seusianya, atau dengan kata lain, tinggi badan anak berada di bawah standar. Standar yang dipakai sebagai acuan adalah kurva pertumbuhan yang dibuat oleh Badan Kesehatan Dunia (WHO). Tujuan dari kegiatan ini adalah meningkatnya pengetahuan masyarakat terkait stunting dan dampaknya serta cara penanggulangannya. Metode yang digunakan dalam kegiatan ini berupa ceramah dan diskusi. Dengan jumlah peserta penyuluhan sebanyak 25 warga. Hasil yang didapatkan setelah dilakukan penyuluhan yaitu tingkat pengetahuan warga meningkat mengenai stunting dan dampaknya serta cara penanggulangannya, Saran kepada warga diharapkan dapat menyebarluaskan informasi yang telah didapatkan kepada masyarakat lainnya.
Modal Sosial Kelompok Langgak Craft Desa Koto Tandun Kecamatan Tandun Kabupaten Rokan Hulu
Authors: Masdelina Masdelina , Asriwandari Hesti , Nurhamlin Nurhamlin 
Published: March 2024

The wealth of local potential existing in a community can inspire creativity which can create some opportunities for the community through establishing home-based businesses or what is now known as Home Industry. IKM Langgak Craft group is able to construct a palm oil stick waste processing plant considered as having contributing ability in the long term. The objective of current research is to analyze the elements of social capital contributing and the weaknesses of each social capital element in the IKM Langgak Craft group in Koto Tandun Village, Tandun Sub District, Rokan Hulu Regency. This research used Putnam’s theory stating that social capital includes (1) trust, (2) norm/ethics, and (3) network. This research used qualitative methodology with purposive sampling technique; the author also lived in the location of research for 2 (two) months. The result of research analyzing the Bonding of IKM Langgak Craft group shows that the group has high solidarity. Strong solidarity social care), mutual help, and support for each other create emotional relation in the IKM Langgak Craft group as shown with sympathy and empathy appearing in the group. Bridging Social Capital can be seen from the net wok relation between IKM Langgak Craft group and other groups or IKMs; through this network, the IKM Langgak Craft group will exchange ideas and information on how to produce business innovation and other varying common knowledge. The Linking Social Capital of IKM Langgak Craft group has fairly broad network that has been created since 2019-2022 with both governmental institutions and private companies around it.
Cellular abundance-based prognostic model associated with deregulated gene expression of leukemic stem cells in acute myeloid leukemia
Authors: Han Dong-Jin , Kim Sunmin , Lee Seo-Young , Kang Su Jung , Moon Youngbeen , Kim Hoon Seok , Kim Myungshin , Kim Tae-Min 
Published: March 2024

Background: Previous studies have reported that genes highly expressed in leukemic stem cells (LSC) may dictate the survival probability of patients and expression-based cellular deconvolution may be informative in forecasting prognosis. However, whether the prognosis of acute myeloid leukemia (AML) can be predicted using gene expression and deconvoluted cellular abundances is debatable.Methods: Nine different cell-type abundances of a training set composed of the AML samples of 422 patients, were used to build a model for predicting prognosis by least absolute shrinkage and selection operator Cox regression. This model was validated in two different validation sets, TCGA-LAML and Beat AML (n = 179 and 451, respectively).Results: We introduce a new prognosis predicting model for AML called the LSC activity (LSCA) score, which incorporates the abundance of 5 cell types, granulocyte-monocyte progenitors, common myeloid progenitors, CD45RA + cells, megakaryocyte-erythrocyte progenitors, and multipotent progenitors. Overall survival probabilities between the high and low LSCA score groups were significantly different in TCGA-LAML and Beat AML cohorts (log-rank p-value = 3.3×10−4 and 4.3×10−3, respectively). Also, multivariate Cox regression analysis on these two validation sets shows that LSCA score is independent prognostic factor when considering age, sex, and cytogenetic risk (hazard ratio, HR = 2.17; 95% CI 1.40–3.34; p &lt; 0.001 and HR = 1.20; 95% CI 1.02–1.43; p &lt; 0.03, respectively). The performance of the LSCA score was comparable to other prognostic models, LSC17, APS, and CTC scores, as indicated by the area under the curve. Gene set variation analysis with six LSC-related functional gene sets indicated that high and low LSCA scores are associated with upregulated and downregulated genes in LSCs.Conclusion: We have developed a new prognosis prediction scoring system for AML patients, the LSCA score, which uses deconvoluted cell-type abundance only.
Impact of head-down-tilt body position on abdomen resistance for urinary bladder monitory applications
Authors: Freeborn Todd J. , Critcher Shelby , Hooper Gwendolyn L. 
Published: March 2024

Tissue electrical impedance (or bioimpedance) is a quantity related to the passive, frequency-dependent electrical properties of a biological tissue and is a promising modality for continuous monitoring of relative bladder volume and bladder activity. In this study, the impact of body position [specifically 6° head-down tilt (HDT)] intended to induce fluid redistribution and, therefore, result in a change in the electrical resistance of the abdomen is evaluated. The abdomen resistance (10 kHz–100 kHz) of nine healthy young adults was measured before and after 240 min in a 6° HDT position. Over this period, the resistance increase was not statistically significant even though the average bladder volume increased by 506 mL. It was expected that the abdomen resistance would decrease with an increase in bladder volume over this period. The masking of the expected resistance decrease is attributed to the shift in the fluid from the legs/abdomen to the neck/chest caused by the HDT body position over this period. Overall, this suggests that methods to differentiate bladder volume changes from other types of fluid shifts in the body are needed for resistance-based monitoring under free-living conditions.
Prediction of immunotherapy response in idiopathic membranous nephropathy using deep learning-pathological and clinical factors
Authors: Wei Xuejiao , Long Mengtuan , Fan Zhongyu , Hou Yue , Zhu Xiaoyu , Qu Zhihui , Du Yujun 
Published: March 2024

BackgroundOwing to individual heterogeneity, patients with idiopathic membranous nephropathy (IMN) exhibit varying sensitivities to immunotherapy. This study aimed to establish and validate a model incorporating pathological and clinical features using deep learning training to evaluate the response of patients with IMN to immunosuppressive therapy.MethodsThe 291 patients were randomly categorized into training (n = 219) and validation (n = 72) cohorts. Patch-level convolutional neural network training in a weakly supervised manner was utilized to analyze whole-slide histopathological features. We developed a machine-learning model to assess the predictive value of pathological signatures compared to clinical factors. The performance levels of the models were evaluated using the area under the receiver operating characteristic curve (AUC) on the training and validation tests, and the prediction accuracies of the models for immunotherapy response were compared.ResultsMultivariate analysis indicated that diabetes and smoking were independent risk factors affecting the response to immunotherapy in IMN patients. The model integrating pathologic features had a favorable predictive value for determining the response to immunotherapy in IMN patients, with AUCs of 0.85 and 0.77 when employed in the training and test cohorts, respectively. However, when incorporating clinical features into the model, the predictive efficacy diminishes, as evidenced by lower AUC values of 0.75 and 0.62 on the training and testing cohorts, respectively.ConclusionsThe model incorporating pathological signatures demonstrated a superior predictive ability for determining the response to immunosuppressive therapy in IMN patients compared to the integration of clinical factors.
Effectiveness and safety of glucagon-like peptide 1 receptor agonists in patients with type 2 diabetes: evidence from a retrospective real-world study
Authors: Jiang Yan , Bai Han-Sheng , Liu Guo-Xin , Wang Shi-Yi , Yin Li , Hou Zhao-Ting , Zhao Chen-Yang , Fan Guang-Jun 
Published: March 2024

IntroductionGlobal phase III clinical trials have shown superior hypoglycemic efficacy to insulin and other oral hypoglycemic agents. However, there is a scarcity of real-world data comparing different glucagon-like peptide 1 receptor agonist (GLP-1RA) directly. This study aimed to assess the safety and effectiveness of various GLP-1RA in treating type 2 diabetes mellitus (T2DM) in a real-world clinical setting and identify predictive factors for favorable treatment outcomes.MethodsThis was a retrospective, single-center, real-world study. The changes in HbA1c, fasting plasma glucose (FPG), body weight, systolic blood pressure (SBP), diastolic blood pressure (DBP), and the percentage of participants who achieved HbA1c of &lt;7%, 7%-8%, and ≥ 8% after GLP-1RA treatment was analyzed. The clinical factors that affect the effectiveness of GLP-1RA were analyzed.ResultsAt baseline, the 249 participants had a mean baseline HbA1c of 8.7 ± 1.1%. After at least three months of follow-up, the change in HbA1c was -0.89 ± 1.3% from baseline. Dulaglutide exerted a more significant hypoglycemic effect than immediate-release exenatide. The percentage of participants who achieved HbA1c&lt;7% was substantial, from 6.0% at baseline to 28.9%. Average body weight decreased by 2.02 ± 3.8 kg compared to baseline. After GLP-1RA treatment, the reduction in SBP was 2.4 ± 7.1 mmHg from baseline. A shorter duration of diabetes and a higher baseline HbA1c level were more likely to achieve a good response in blood glucose reduction.ConclusionsThis study provided real-world evidence showing that GLP-1RA significantly improved HbA1c, body weight, and SBP. The results can inform the decision-making about GLP-1RA treatment in daily clinical practice.
Structure-guided engineering and molecular simulations to design a potent monoclonal antibody to target aP2 antigen for adaptive immune response instigation against type 2 diabetes
Authors: Khan Abbas , Zahid Muhammad Ammar , Mohammad Anwar , Agouni Abdelali 
Published: March 2024

IntroductionDiabetes mellitus (DM) is recognized as one of the oldest chronic diseases and has become a significant public health issue, necessitating innovative therapeutic strategies to enhance patient outcomes. Traditional treatments have provided limited success, highlighting the need for novel approaches in managing this complex disease.MethodsIn our study, we employed graph signature-based methodologies in conjunction with molecular simulation and free energy calculations. The objective was to engineer the CA33 monoclonal antibody for effective targeting of the aP2 antigen, aiming to elicit a potent immune response. This approach involved screening a mutational landscape comprising 57 mutants to identify modifications that yield significant enhancements in binding efficacy and stability.ResultsAnalysis of the mutational landscape revealed that only five substitutions resulted in noteworthy improvements. Among these, mutations T94M, A96E, A96Q, and T94W were identified through molecular docking experiments to exhibit higher docking scores compared to the wild-type. Further validation was provided by calculating the dissociation constant (KD), which showed a similar trend in favor of these mutations. Molecular simulation analyses highlighted T94M as the most stable complex, with reduced internal fluctuations upon binding. Principal components analysis (PCA) indicated that both the wild-type and T94M mutant displayed similar patterns of constrained and restricted motion across principal components. The free energy landscape analysis underscored a single metastable state for all complexes, indicating limited structural variability and potential for high therapeutic efficacy against aP2. Total binding free energy (TBE) calculations further supported the superior performance of the T94M mutation, with TBE values demonstrating the enhanced binding affinity of selected mutants over the wild-type.DiscussionOur findings suggest that the T94M substitution, along with other identified mutations, significantly enhances the therapeutic potential of the CA33 antibody against DM by improving its binding affinity and stability. These results not only contribute to a deeper understanding of antibody-antigen interactions in the context of DM but also provide a valuable framework for the rational design of antibodies aimed at targeting this disease more effectively.
An updated management approach of Pompe disease patients with high-sustained anti-rhGAA IgG antibody titers: experience with bortezomib-based immunomodulation
Authors: Desai Ankit K. , Shrivastava Garima , Grant Christina L. , Wang Raymond Y. , Burt Trevor D. , Kishnani Priya S. 
Published: March 2024

IntroductionHigh sustained anti-rhGAA antibody titers (HSAT; ≥12,800) are directly linked to reduced efficacy of enzyme replacement therapy (ERT) and subsequent clinical deterioration in infantile-onset Pompe disease (IOPD). We have previously demonstrated the safety and effectiveness of a bortezomib-based immune-tolerance induction (ITI) regimen (bortezomib, rituximab, methotrexate, and IVIG) in eliminating HSAT.MethodsHere, we describe two IOPD cases (patients 6 and 8) who developed HSAT at 8 and 10 weeks on ERT despite transient low-dose methotrexate ITI administration in the ERT-naïve setting and were treated with a bortezomib-based ITI regimen, and we compare their courses to a series of six historical patients (patients 1-5, and 7) with a similar presentation who exemplify our evolving approach to treatment.ResultsIn total, patients 6 and 8 received 16 and 8 doses of bortezomib (4 doses=1 cycle) respectively reducing titers from 25,600 to seronegative, but differences in the course of their therapy were instructive regarding the optimal approach to initial treatment of HSAT; specifically, patient 6 was treated initially with only a single course of bortezomib rescue therapy, while patient 8 received two back-to-back courses. Patient 8 received IVIG therapy throughout the immunosuppression whereas patient 6 received IVIG therapy and was switched to subcutaneous IgG replacement. Patient 6 had a transient reduction in anti-rhGAA antibodies, after receiving a single initial cycle of bortezomib, but had a recurrence of high anti-rhGAA antibody titer after 160 weeks that required 3 additional cycles of bortezomib to ultimately achieve tolerance. In contrast, patient 8 achieved tolerance after being given two consecutive cycles of bortezomib during their initial treatment and had B cell recovery by week 54. Since the reduction in anti-rhGAA antibodies, both patients are doing well clinically, and have decreasing ALT, AST, and CK. No major infections leading to interruption of treatment were observed in either patient. The bortezomib-based ITI was safe and well-tolerated, and patients continue to receive ERT at 40 mg/kg/week.DiscussionThese case studies and our previous experience suggest that to achieve an effective reduction of anti-rhGAA antibodies in the setting of HSAT, bortezomib should be initiated at the earliest sign of high anti-rhGAA antibodies with a minimum of two consecutive cycles as shown in the case of patient 8. It is important to note that, despite initiation of ERT at age 2.3 weeks, patient 8 quickly developed HSAT. We recommend close monitoring of anti-rhGAA antibodies and early intervention with ITI as soon as significantly elevated anti-rhGAA antibody titers are noted.
Mortality of continuous infusion versus intermittent bolus of meropenem: a systematic review and meta-analysis of randomized controlled trials
Authors: Ai Ming-Ying , Chang Wei-Lun , Liu Chia-Ying 
Published: March 2024

BackgroundMeropenem belongs to the carbapenem class, which is categorized as beta-lactam antibiotics. These antibiotics are administered in intermittent bolus doses at specific time intervals. However, the continuous infusion approach ensures sustained drug exposure, maintaining the drug concentration above the minimum inhibitory concentration (MIC) throughout the entire treatment period. This study aimed to find out the association between continuous infusions of meropenem and mortality rates.Materials and methodsWe conducted a search of the PubMed/Medline, EMBASE, Cochrane Central, and ClinicalTrials.gov databases up to 14 August 2023. The six randomized controlled trials (RCTs) were identified and included in our analysis. The random-effects model was implemented using Comprehensive Meta-Analysis software to examine the outcomes.ResultsOur study included a total of 1,529 adult patients from six randomized controlled trials. The primary outcome indicated that continuous infusion of meropenem did not lead to reduction in the mortality rate (odds ratio = 0.844, 95% CI: 0.671–1.061, P =0.147). Secondary outcomes revealed no significant differences in ICU length of stay (LOS), ICU mortality, clinical cure, or adverse events between continuous infusion and traditional intermittent bolus strategies of meropenem. Notably, we observed significant improvements in bacterial eradication (odds ratio 19 = 2.207, 95% CI: 1.467–3.320, P &lt; 0.001) with continuous infusion of meropenem. Our study also suggested that performing continuous infusion may lead to better bacterial eradication effects in resistant pathogens (coefficient: 2.5175, P = 0.0138*).ConclusionContinuous infusion of meropenem did not result in the reduction of mortality rates but showed potential in improving bacterial eradication. Furthermore, this strategy may be particularly beneficial for achieving better bacterial eradication, especially in cases involving resistant pathogens.
Metabolomic profiling reveals altered phenylalanine metabolism in Parkinson’s disease in an Egyptian cohort
Authors: Shebl Nourhan , El-Jaafary Shaimaa , Saeed Ayman A. , Elkafrawy Passent , El-Sayed Amr , Shamma Samir , Elnemr Rasha , Mekky Jaidaa , Mohamed Lobna A. , Kittaneh Omar , El-Fawal Hassan , Rizig Mie , Salama Mohamed 
Published: March 2024

Introduction: Parkinson’s disease (PD) is the most common motor neurodegenerative disease worldwide. Given the complexity of PD etiology and the different metabolic derangements correlated to the disease, metabolomics profiling of patients is a helpful tool to identify patho-mechanistic pathways for the disease development. Dopamine metabolism has been the target of several previous studies, of which some have reported lower phenylalanine and tyrosine levels in PD patients compared to controls.Methods: In this study, we have collected plasma from 27 PD patients, 18 reference controls, and 8 high-risk controls to perform a metabolomic study using liquid chromatography-electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS).Results: Our findings revealed higher intensities of trans-cinnamate, a phenylalanine metabolite, in patients compared to reference controls. Thus, we hypothesize that phenylalanine metabolism has been shifted to produce trans-cinnamate via L-phenylalanine ammonia lyase (PAL), instead of producing tyrosine, a dopamine precursor, via phenylalanine hydroxylase (PAH).Discussion: Given that these metabolites are precursors to several other metabolic pathways, the intensities of many metabolites such as dopamine, norepinephrine, and 3-hydroxyanthranilic acid, which connects phenylalanine metabolism to that of tryptophan, have been altered. Consequently, and in respect to Metabolic Control Analysis (MCA) theory, the levels of tryptophan metabolites have also been altered. Some of these metabolites are tryptamine, melatonin, and nicotinamide. Thus, we assume that these alterations could contribute to the dopaminergic, adrenergic, and serotonergic neurodegeneration that happen in the disease.
Age-related differences in affective behaviors in mice: possible role of prefrontal cortical-hippocampal functional connectivity and metabolomic profiles
Authors: Febo Marcelo , Mahar Rohit , Rodriguez Nicholas A. , Buraima Joy , Pompilus Marjory , Pinto Aeja M. , Grudny Matteo M. , Bruijnzeel Adriaan W. , Merritt Matthew E. 
Published: March 2024

IntroductionThe differential expression of emotional reactivity from early to late adulthood may involve maturation of prefrontal cortical responses to negative valence stimuli. In mice, age-related changes in affective behaviors have been reported, but the functional neural circuitry warrants further investigation.MethodsWe assessed age variations in affective behaviors and functional connectivity in male and female C57BL6/J mice. Mice aged 10, 30 and 60 weeks (wo) were tested over 8 weeks for open field activity, sucrose preference, social interactions, fear conditioning, and functional neuroimaging. Prefrontal cortical and hippocampal tissues were excised for metabolomics.ResultsOur results indicate that young and old mice differ significantly in affective behavioral, functional connectome and prefrontal cortical-hippocampal metabolome. Young mice show a greater responsivity to novel environmental and social stimuli compared to older mice. Conversely, late middle-aged mice (60wo group) display variable patterns of fear conditioning and during re-testing in a modified context. Functional connectivity between a temporal cortical/auditory cortex network and subregions of the anterior cingulate cortex and ventral hippocampus, and a greater network modularity and assortative mixing of nodes was stronger in young versus older adult mice. Metabolome analyses identified differences in several essential amino acids between 10wo mice and the other age groups.DiscussionThe results support differential expression of ‘emotionality’ across distinct stages of the mouse lifespan involving greater prefrontal-hippocampal connectivity and neurochemistry.