bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Continuum of Sudden Stratospheric Warmings
Authors: Coughlin K. , Gray L. J. 
Published: August 2008

Abstract
The k-means cluster technique is used to examine 43 yr of daily winter Northern Hemisphere (NH) polar stratospheric data from the 40-yr ECMWF Re-Analysis (ERA-40). The results show that the NH winter stratosphere exists in two natural well-separated states. In total, 10% of the analyzed days exhibit a warm disturbed state that is typical of sudden stratospheric warming events. The remaining 90% of the days are in a state typical of a colder undisturbed vortex. These states are determined objectively, with no preconceived notion of the groups. The two stratospheric states are described and compared with alternative indicators of the polar winter flow, such as the northern annular mode. It is shown that the zonally averaged zonal winds in the polar upper stratosphere at ∼7 hPa can best distinguish between the two states, using a threshold value of ∼4 m s−1, which is remarkably close to the standard WMO criterion for major warming events. The analysis also determines that there are no further divisions within the warm state, indicating that there is no well-designated threshold between major and minor warmings, nor between split and displaced vortex events. These different manifestations are simply members of a continuum of warming events.
The Role of SST in the East Pacific Warm Pool in the Interannual Variability of Central American Rainfall
Authors: Karnauskas Kristopher B. , Busalacchi Antonio J. 
Published: August 2008

Abstract
Satellite- and gauge-based precipitation and sea surface temperature (SST) are analyzed to understand the role of SST in the east Pacific warm pool (EPWP) in the interannual variability of Central American rainfall. It is shown that, during the rainy season following the mature phase of an El Niño event, an anomalously warm EPWP can cause a rapid enhancement of the eastern Pacific intertropical convergence zone (EP ITCZ), which directly leads to a positive rainfall anomaly over Central America. Moreover, the timing and amplitude of the SST-enhanced EP ITCZ depends on the persistence of the El Niño event. The longer the equatorial SST anomaly persists, the longer the EPWP is subject to anomalous shortwave heating, and thus the stronger (and later) the subsequent SST enhancement of the EP ITCZ. The implications for regional climate monitoring and predictability are explored; potential predictability of seasonal rainfall is demonstrated 4 months in advance using an SST-based index designed to capture the essence of the above-mentioned mechanism.
The Moist Static Energy Budget of a Composite Tropical Intraseasonal Oscillation in a Climate Model
Authors: Maloney Eric D. 
Published: August 2008

Abstract
The intraseasonal moist static energy (MSE) budget is analyzed in a climate model that produces realistic eastward-propagating tropical intraseasonal wind and precipitation variability. Consistent with the recharge–discharge paradigm for tropical intraseasonal variability, a buildup of column-integrated MSE occurs within low-level easterly anomalies in advance of intraseasonal precipitation, and a discharge of MSE occurs during and after precipitation when westerly anomalies occur. The strongest MSE anomalies peak in the lower troposphere and are, primarily, regulated by specific humidity anomalies.
The leading terms in the column-integrated intraseasonal MSE budget are horizontal advection and surface latent heat flux, where latent heat flux is dominated by the wind-driven component. Horizontal advection causes recharge (discharge) of MSE within regions of anomalous equatorial lower-tropospheric easterly (westerly) anomalies, with the meridional component of the moisture advection dominating the MSE budget near 850 hPa. Latent heat flux anomalies oppose the MSE tendency due to horizontal advection, making the recharge and discharge of column MSE more gradual than if horizontal advection were acting alone. This relationship has consequences for the time scale of intraseasonal variability in the model.
Eddies dominate intraseasonal meridional moisture advection in the model. During periods of low-level intraseasonal easterly anomalies, eddy kinetic energy (EKE) is anomalously low due to a suppression of tropical synoptic-scale disturbances and other variability on time scales shorter than 20 days. Anomalous moistening of the equatorial lower troposphere occurs during intraseasonal easterly periods through suppression of eddy moisture advection between the equator and poleward latitudes. During intraseasonal westerly periods, EKE is enhanced, leading to anomalous drying of the equatorial lower troposphere through meridional advection. Given the importance of meridional moisture advection and wind-induced latent heat flux to the intraseasonal MSE budget, these findings suggest that to simulate realistic intraseasonal variability, climate models must have realistic basic-state distributions of lower-tropospheric zonal wind and specific humidity.
Ground-Based Passive Microwave Profiling during Dynamic Weather Conditions
Authors: Knupp K. R. , Coleman T. , Phillips D. , Ware R. , Cimini D. , Vandenberghe F. , Vivekanandan J. , Westwater E. 
Published: August 2008

Abstract
Short-period (1–5 min) temperature and humidity soundings up to 10-km height are retrieved from ground-based 12-channel microwave radiometer profiler (MWRP) observations. In contrast to radiosondes, the radiometric retrievals provide very high temporal resolution (1 min or less) of thermodynamic profiles, but the vertical resolution, which declines in proportion to the height above ground level, is lower. The high temporal resolution is able to resolve detailed meso-γ-scale thermodynamic and limited microphysical features of various rapidly changing mesoscale and/or hazardous weather phenomena. To illustrate the MWRP capabilities and potential benefits to research and operational activities, the authors present example radiometric retrievals from a variety of dynamic weather phenomena including upslope supercooled fog, snowfall, a complex cold front, a nocturnal bore, and a squall line accompanied by a wake low and other rapid variations in low-level water vapor and temperature.
Genome-wide profiling of follicular lymphoma by array comparative genomic hybridization reveals prognostically significant DNA copy number imbalances
Authors: Cheung K.-John J. , Shah Sohrab P. , Steidl Christian , Johnson Nathalie , Relander Thomas , Telenius Adele , Lai Betty , Murphy Kevin P. , Lam Wan , Al-Tourah Abdulwahab J. , Connors Joseph M. , Ng Raymond T. , Gascoyne Randy D. , Horsman Douglas E. 
Published: August 2008

Abstract
The secondary genetic events associated with follicular lymphoma (FL) progression are not well defined. We applied genome-wide BAC array comparative genomic hybridization to 106 diagnostic biopsies of FL to characterize regional genomic imbalances. Using an analytical approach that defined regions of copy number change as intersections between visual annotations and a Hidden Markov model–based algorithm, we identified 71 regional alterations that were recurrent in at least 10% of cases. These ranged in size from approximately 200 kb to 44 Mb, affecting chromosomes 1, 5, 6, 7, 8, 10, 12, 17, 18, 19, and 22. We also demonstrated by cluster analysis that 46.2% of the 106 cases could be sub-grouped based on the presence of +1q, +6p/6q−, +7, or +18. Survival analysis showed that 21 of the 71 regions correlated significantly with inferior overall survival (OS). Of these 21 regions, 16 were independent predictors of OS using a multivariate Cox model that included the international prognostic index (IPI) score. Two of these 16 regions (1p36.22-p36.33 and 6q21-q24.3) were also predictors of transformation risk and independent of IPI. These prognostic features may be useful to identify high-risk patients as candidates for risk-adapted therapies.
Prognostic significance of CD20 expression in adults with de novo precursor B-lineage acute lymphoblastic leukemia
Authors: Thomas Deborah A. , O'Brien Susan , Jorgensen Jeffrey L. , Cortes Jorge , Faderl Stefan , Garcia-Manero Guillermo , Verstovsek Srdan , Koller Charles , Pierce Sherry , Huh Yang , Wierda William , Keating Michael J. , Kantarjian Hagop M. 
Published: August 2008

AbstractImmunophenotypic classification of acute lymphoblastic leukemia (ALL) has well-recognized prognostic implications. The significance of CD20 expression has been evaluated in childhood precursor B-lineage ALL with conflicting results. We retrospectively analyzed the influence of CD20 expression on outcome in 253 adults with de novo precursor B-lineage ALL treated with either conventional (VAD/CVAD) or intensive (hyper-CVAD) frontline chemotherapy regimens in the pre-rituximab era. Overall, CD20 positivity of at least 20% was associated with lower 3-year rates of complete remission duration (CRD; 20% vs 55%, P < .001) and overall survival (OS; 27% vs 40%, p = .03). In the CD20 negative subset, the 3-year rates for CRD (58% vs 42%, p = .04) and OS (60% vs 28%, P < .001) were superior for hyper-CVAD compared with VAD/CVAD; rates were particularly favorable for the CD20 negative younger age group (68% and 85%, respectively). In contrast, 3-year CRD and OS rates were uniformly poor for the CD20-positive group regardless of therapy (27% or less). Multivariate analysis for event-free survival identified older age, leukocyte count higher than 30 × 109/L, presence of Philadelphia chromosome, high systemic risk classification, and CD20 positivity as independent predictors of worse outcome. In conclusion, CD20 expression in de novo adult precursor B-lineage ALL appears to be associated with a poor prognosis. Incorporation of monoclonal antibodies directed against CD20 into frontline chemotherapy regimens warrants investigation.
Association of narcolepsy-cataplexy with HLA-DRB1 and DQB1in Mexican patients: A relationship between HLA and gender is suggested
Authors: Alaez Carmen , Lin Ling , Flores-A Hilario , Vazquez Miriam , Munguia Andrea , Mignot Emmanuel , Haro Reyes , Baker Harry , Gorodezky Clara 
Published: August 2008

Abstract

Background
Narcolepsy-cataplexy is characterized by excessive daytime sleepiness with recurrent episodes of irresistible sleep, cataplexy, hallucinations and sleep paralysis. Its aetiology is unknown, but it is positively associated with the human leukocyte antigens (HLA) in all studied populations. The purpose of the present study was to investigate the association of HLA class II DRB1/DQB1 alleles with narcolepsy-cataplexy in Mexican Mestizo patients.

Methods
This is a case-control study of consecutive patients and ethnically matched controls. We included 32 patients diagnosed with typical narcolepsy-cataplexy, of the National Institute of Neurology, of the Institute of Psychiatry and at the Center of Narcolepsy at Stanford University. As healthy controls, 203 Mexican Mestizos were included. DRB1 alleles were identified using sequence based typing. A PCR-SSOP reverse dot blot was used for DQB1 typing. Allele frequency was calculated by direct counting and the significance of the differences was assessed using the Yates Chi square. Odds ratio and confidence intervals were evaluated.

Results
HLA-DRB1*1501 (OR = 8.2; pc < 0.0001) and DQB1*0602 (OR = 8.4; pc < 0.0001) were found positively associated with narcolepsy. When deleting DQB1*0602+ patients from the analysis, DQB1*0301 was also found increased (OR = 2.7; p = 0.035; pc = NS). DQB1*0602/DQB1*0301 genotype was present in 15.6% of the cases (OR = 11.5; p = 0.00035), conferring a high risk. DRB1*0407 (OR = 0.2; p = 0.016 pc = NS) and DQB1*0302(OR = 0.4; p = 0.017, pc = NS) were found decreased in the patients. The gender stratification analysis showed a higher risk in females carrying DRB1*1501 (OR = 15.8, pc < 0.0001) and DQB1*0602 (OR = 19.8, pc < 0.0001) than in males (OR = 5.0 for both alleles; p = 0.012, pc = NS for DRB1 & p = 0.0012, pc = 0.017 for DQB1). The susceptibility alleles found in Mexicans with narcolepsy are also present in Japanese and Caucasians; DRB1*04 linked protection has also been shown in Koreans. A stronger HLA association is suggested in females, in accordance with the sexual dimorphism claimed previously.

Conclusion
This knowledge may contribute to a better understanding of the disease pathogenesis in different populations. The evaluation of the risk to develop narcolepsy-cataplexy in carriers of the described alleles/genotypes may also be possible. A larger sample should be analysed in Mexican and in other Hispanic patients to confirm these results.
Prothrombin complex concentrate (Beriplex P/N) in severe bleeding: experience in a large tertiary hospital
Authors: Bruce David , Nokes Tim JC 
Published: August 2008

Abstract
Introduction
Major blood loss can often be life-threatening and is most commonly encountered in the settings of surgery and trauma. Patients receiving anticoagulant therapy are also at increased risk of bleeding. We investigated the use of a prothrombin complex concentrate (PCC; Beriplex P/N, CSL Behring, Marburg, Germany) to treat severe bleeding in a variety of settings: cardiac surgery, warfarin therapy and other surgery.

Methods
Thirty consecutive patients who had received PCC were identified from blood transfusion records. For cardiac surgery and warfarin reversal, PCC was administered in accordance with hospital protocols. PCC was administered to cardiac and other surgical patients responding poorly to recognized blood products, whereas it was administered first-line to patients with life-threatening bleeds and requiring warfarin reversal, in accordance with British Committee for Standards in Haematology guidelines. We conducted a retrospective analysis of patient records in order to ascertain PCC dose, use of other blood products and response to PCC (clotting screen results before and after PCC administration, haemostasis achievement, and survival).

Results
Six patients (20%) were excluded because of inadequate documentation (n = 5) or acquired haemophilia (n = 1). Therefore, 24 patients were included in the analysis: coronary artery bypass graft (n = 5), mitral/aortic valve replacement (n = 2), other surgery (n = 9) and warfarin reversal (n = 8). Most patients (83.3%) received no more than 1500 IU of Beriplex P/N 500. Considerable reduction in administration of other blood products was seen during the 24 hours after PCC administration. Partial or complete haemostasis was achieved in 14 out of 18 cases (77.8%). In total, 12 out of 24 patients (50%) died during the study; two-thirds of the deaths were considered unrelated to bleeding. No thrombotic complications or adverse drug reactions were observed.

Conclusion
This study emphasizes the value of PCC in reversing the effects of oral anticoagulant therapy in bleeding patients. It also demonstrates the potential value of PCC in controlling bleeding in patients undergoing cardiac and other surgical procedures. The use of PCC in bleeding patients without hereditary or anticoagulation-related coagulopathy is novel, and further investigation is warranted. In the future, it may be possible to use PCC as a substitute for fresh frozen plasma in this setting; adequate documentation is crucial for all blood products.
Distinct chemokine and cytokine gene expression pattern of murine dendritic cells and macrophages in response toMycobacterium tuberculosisinfection
Authors: Jang Sihyug , Uzelac Aleksandra , Salgame Padmini 
Published: August 2008

AbstractIn this study, the early innate cytokine and chemokine response of murine dendritic cells (DCs) and macrophages to Mycobacterium tuberculosis infection was compared. The findings indicate a dissimilar gene expression pattern between the two cell types. The expression of IL-12 and IL-23, important for promoting Th1 and Th17 cells, respectively, was up-regulated only in DCs. In addition, expression of CCL1 and CCL17, which are important in recruitment of T regulatory cells, was DC-specific, as was the expression of the immunosuppressive cytokine IL-10. Macrophages, in contrast, exhibited enhanced expression for CCL2 and CXCL10, chemokines that recruit cells to sites of inflammation, and for mycobactericidal molecules NO synthase 2 and TNF. Together, the findings suggest that a component of the innate DC response is not only programmed toward Th1 priming but is also for controlling the magnitude of the Th1 response, and part of the macrophage response is intended for recruiting cells to the lung and for mycobactericidal functions.
Potential prospects of chitosan derivative trimethyl chitosan chloride (TMC) as a polymeric absorption enhancer: synthesis, characterization and applications
Authors: Sahni Jasjeet K , Chopra Shruti , Ahmad Farhan J , Khar Roop K 
Published: August 2008

Abstract
In recent years, researchers have been working extensively on various novel properties of polymers to develop increased efficiency of drug delivery and improve bioavailability of various drug molecules, especially macromolecules. Chitosan, a naturally occurring polysaccharide, because of its protonated/polymeric nature, provides effective and safe absorption of peptide and protein drugs. Its transmucosal absorption is, however, limited to acidic media because of its strong intermolecular hydrogen bonds. A new partially quaternized chitosan derivative, N-trimethyl chitosan chloride (TMC), has been synthesized with improved solubility, safety and effectiveness as an absorption enhancer at neutral pH and in aqueous environment. It enhances the absorption, especially of peptide drugs, by reversible opening of tight junctions in between epithelial cells, thereby facilitating the paracellular diffusion of peptide drugs. This derivative thus opens new perspectives as a biomaterial for various pharmaceutical applications/drug delivery systems. This review deals with the potential use of the quaternized chitosan derivative as a permeation enhancer for the mucosal delivery of macromolecular drugs along with its other biomedical applications.
An investigation into solvent-membrane interactions when assessing drug release from organic vehicles using regenerated cellulose membranes
Authors: Reid Monica L , Brown Marc B , Moss Gary P , Jones Stuart A 
Published: August 2008

Abstract
The influence of organic solvents on artificial membranes when assessing drug release from topical formulations is, generally, poorly characterised yet current guidelines require no characterisation of the membrane before, during or after an experiment. Therefore, the aim of this study was to determine the effect of solvent-membrane interactions when using in-vitro Franz cell methods for the assessment of corticosteroid release and to assess compliance or otherwise with Higuchi's equation. The rate of beclometasone dipropionate monohydrate (BDP) and betamethasone 17-valerate (BMV) release across a regenerated cellulose membrane (RCM), from both saturated solutions and commercial formulations, was determined. Increasing the ratio of organic solvent, compared with aqueous phase, in the donor fluid (DF) resulted in up to a 416-fold increase in steady-state flux. Further, alterations in the receiver fluid (RF) composition caused, in some cases, 337-fold increases in flux. Analysis indicated that the RCM remained chemically unchanged, that its pore size remained constant and that no drug partitioned into the membrane, regardless of the DF or RF employed. However, it was observed that the organic solvents had a thinning effect on the RCM, resulting in enhanced flux, which was potentially due to the variation in the diffusional path length. Such findings raise issues of the veracity of data produced from any membrane release study involving a comparison of formulations with differing solvent content.
Distribution of an intravenous injectable nimodipine nanosuspension in mice
Authors: Xiong Ruolan , Lu Weigen , Yue Peng , Xu Rong , Li Jun , Chen Tingting , Wang Peiquan 
Published: August 2008

Abstract
The distribution of an intravenous injectable nimodipine nanosuspension with mean particle size of both 300 and 650 nm in mice was systemically investigated compared with that of a nimodipine ethanol formulation (Nimotop) and a nanosuspension coated with Tween-80. The results showed that the 650-nm nanoparticles provided significantly higher drug concentrations in the liver, spleen and lungs because of their capture by Kupffer cells in the mononuclear phagocyte system, but lower drug concentrations in the brain compared with Nimotop and smaller nanoparticles. These nanoparticles failed to give increased brain concentrations even when coated with Tween-80. The 300-nm nanoparticles could effectively increase drug concentrations in the brain and remarkably reduce drug concentrations in the liver, spleen and lungs, indicating that the nimodipine nanosuspension may be a promising formulation with no ethanol, but the particle size must be small.
Metabolic effects of various antidiabetic and hypolipidaemic agents on a high-fat diet and multiple low-dose streptozocin (MLDS) mouse model of diabetes
Authors: Arulmozhi Dakshinamoorty Kandasamy , Kurian Rubin , Bodhankar Subodh L , Veeranjaneyulu Addepalli 
Published: August 2008

Abstract
Insulin resistance and subsequent insulin secretory defect are two main features of type 2 diabetes and associated metabolic disorders. The animal models of type 2 diabetes are very complex and are as heterogeneous as the disease. We have evaluated the effect of various antidiabetic and lipid lowering agents (fenofibrate, rosiglitazone, glimepiride, metformin and simvastatin) on the metabolic abnormalities induced by combining a high-fat diet and multiple low-dose streptozocin (MLDS) in mice. Male Swiss albino mice were orally treated with the above agents and fed with a diet containing high fat for 28 days. On day 15 the animals were injected intraperitoneally with low-dose streptozocin (40 mg kg−1), which was administered for five consecutive days. At the end of the 28-day treatment plasma metabolic parameters (glucose, triglyceride and immunoreactive insulin) were estimated. The antidiabetic and hypolipidaemic agents exhibited differential effects on these metabolic parameters. With the exception of fenofibrate all these agents reduced the plasma glucose levels, and the effects of metformin and rosiglitazone on glucose were found to be statistically significant. Although the effect of the test drugs on cholesterol was modest, a significant decrease in triglyceride levels was observed with sub-chronic treatment with these agents. Interestingly, glimepiride mildly elevated the insulin levels while the other antidiabetics and hypolipidaemics reduced the insulin levels, with metformin and rosiglitazone exhibiting statistically significant effects on insulin. To our knowledge this is the first report on the effect of various peroxisome proliferator-activated receptor modulators and newer antidiabetics on the metabolic effects induced by the combined high-fat diet and MLDS model of type 2 diabetes in Swiss albino mice. The results suggested the complexity of the hyperglycaemia, hyperinsulinaemia and hypertriglyceridaemia induced by the high-fat diet and MLDS mouse model, and their correction by various antidiabetics and antihyperlipidaemics may have involved diverse mechanisms.