bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen‐induced arthritis as a model of hyperalgesia: Functional and cellular analysis of the analgesic actions of tumor necrosis factor blockade
Authors: Inglis Julia J. , Notley Clare A. , Essex David , Wilson Alex W. , Feldmann Marc , Anand Praveen , Williams Richard 
Published: November 2007

AbstractObjectiveThere is a disparity in the animal models used to study pain in rheumatoid arthritis (RA), which tends to be acute in nature, and models used to assess the pathogenesis of RA. The latter models, like human RA, are lymphocyte‐driven and polyarthritic. We assessed pain behavior and mechanisms in collagen‐induced arthritis (CIA), the model of preclinical arthritis used most commonly in the field of immunology. We then validated the model using anti–tumor necrosis factor (anti‐TNF) therapy, which has analgesic effects in models of inflammation as well as in human RA.MethodsCIA was induced in DBA/1 mice by immunization with type II collagen at the base of the tail. Swelling and mechanical and thermal hyperalgesia were assessed before and for 28 days after the onset of arthritis. Spontaneous behavior was assessed using an automated activity monitor. Glial activity was assessed by glial fibrillary acidic protein expression, and nerve damage was evaluated by activating transcription factor 3 expression. The actions of anti‐TNF therapy on nociception were then evaluated.ResultsArthritis resulted in a decrease in the threshold for thermal and mechanical stimuli, beginning on the day of onset. Decreased spontaneous activity was also observed. A significant increase in the number of hyperplasic spinal cord astrocytes was observed beginning 10 days after the onset of arthritis. Anti‐TNF therapy was profoundly analgesic, with an efficacy similar to that of cyclooxygenase 2 inhibition, and reduced astrocyte activity in CIA.ConclusionThis study shows that the CIA model is suitable for testing not only antiinflammatory but also analgesic drugs for potential use in RA, and highlights the importance of using appropriate disease models to assess relevant pain pathways.
Type IV collagen α‐chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis
Authors: Poduval P. , Sillat T. , Beklen A. , Kouri V. P. , Virtanen I. , Konttinen Y. T. 
Published: November 2007

AbstractObjectiveNormal synovial lining is composed of macrophage‐like type A and fibroblast‐like type B lining cells. This sheet‐like structure lacks a basement membrane, but its intercellular substance contains some basement membrane components, including type IV collagen. We undertook this study to determine the α‐chain composition of type IV collagen in normal and arthritic synovial lining, using monoclonal α‐chain antibodies.MethodsSamples were analyzed using avidin–biotin–peroxidase complex staining for the presence of collagen α1/2(IV), α3(IV), α4(IV), α5(IV), α6(IV), matrix metalloproteinase 2 (MMP‐2), and MMP‐9, and the enzyme activity was detected using gelatin zymography. Double immunofluorescence was performed for type IV collagen/MMP‐9 and type IV collagen/CD68. Synovial fibroblasts were studied using quantitative reverse transcriptase–polymerase chain reaction.ResultsIn mildly inflamed synovium from 5 trauma patients, α1/2(IV) chains were strongly stained, but α5(IV) and α6(IV) chains were weakly stained. Coding messenger RNA was shown in cultured synovial fibroblasts. Basement membranes of blood vessels contained all α(IV) chains and served as useful positive sample controls. In the synovial lining from 5 patients with rheumatoid arthritis (RA), all α‐chains were absent/very weakly stained. This was coupled with numerous type A lining cells containing MMP‐9 (type IV collagenase), also found in synovial fluid.ConclusionSynovial lining has a unique and very limited α‐chain composition, different from that of the vascular basement membrane, which contains all α‐chains. This special composition and lack of nidogen are probably of relevance for the bidirectional translining diffusion. Such tentative α‐chain–dependent adhesive and transport‐regulating properties seem to be deranged in RA, probably in part due to type IV collagenases produced in the lining and/or released by transmigrating or synovial fluid neutrophils.
Rates of infiltration by macrophages and dendritic cells and expression of interleukin‐18 and interleukin‐12 in the chronic inflammatory lesions of Sjögren's syndrome: Correlation with certain features of immune hyperactivity and factors associated with high risk of lymphoma development
Authors: Manoussakis M. N. , Boiu S. , Korkolopoulou P. , Kapsogeorgou E. K. , Kavantzas N. , Ziakas P. , Patsouris E. , Moutsopoulos H. M. 
Published: November 2007

AbstractObjectiveTo evaluate the expression profile of infiltrating macrophages and dendritic cells (DCs) as well as of interleukin‐18 (IL‐18) and IL‐12 in the minor salivary gland (MSG) lesions of patients with Sjögren's syndrome (SS), and to assess the relationship of these factors with disease parameters.MethodsMacrophages, DCs, T cells, B cells, proIL‐18, mature IL‐18, and IL‐12 were detected by single‐ and double‐labeling immunohistochemistry in MSG specimens from 21 patients with primary SS (13 of 21 tested for IL‐12), 7 patients with secondary SS, and 9 disease control patients. Expression profiles were assessed for correlations with various disease parameters, including adverse predictors of lymphoma development.ResultsMSGs from patients with SS (but not from disease controls) manifested increased infiltration by macrophages and DCs, strong expression of IL‐18 by macrophages (particularly in B cell–rich areas and in germinal center–like structures in primary SS), and expression of IL‐12 by mononuclear cell infiltrates. In primary SS, high infiltration by macrophages correlated with SG enlargement (P = 0.01). The DC infiltration rate correlated positively with the macrophage infiltration rate (P = 0.04), occurrence of SG enlargement (P = 0.03), and presence of C4 hypocomplementemia (P = 0.05), and inversely with serum C4 complement levels (P = 0.001). The rate of infiltration by IL‐18–expressing cells correlated positively with biopsy focus scores (P < 0.001), larger infiltrates of macrophages (P = 0.01), DCs (P = 0.01), and B cells (P = 0.02), and SG enlargement (P = 0.02), and negatively with serum C4 complement levels (P = 0.02). The rate of infiltration by IL‐12–expressing cells correlated inversely with that by IL‐18–expressing cells (P = 0.001), biopsy focus scores (P = 0.003), and SG enlargement (P = 0.01), and positively with serum C4 complement levels (P = 0.05).ConclusionIn patients with primary SS, infiltration of the SG by macrophages and DCs and expression of IL‐18 and IL‐12 appear to play active roles in the expansion and organization of infiltrative injuries and have a correlation with certain predictors of lymphoma development.
Circulating levels of tumor necrosis factor receptors are highly predictive of mortality in patients with rheumatoid arthritis
Authors: Mattey Derek L. , Glossop John R. , Nixon Nicola B. , Dawes Peter T. 
Published: November 2007

AbstractObjectiveTo investigate whether circulating levels of soluble tumor necrosis factor receptors (sTNFR) are predictive of mortality in rheumatoid arthritis (RA).MethodsLevels of sTNFRI and sTNFRII at study entry were quantified using enzyme‐linked immunosorbent assays in sera from 401 white patients with RA followed up for 13 years. Patients were tracked via the National Health Service Central Register, and the relationship between sTNFR levels and mortality was analyzed using a Cox proportional hazards regression model. Hazard ratios (HRs) and 95% confidence intervals (95% CIs) were calculated.ResultsAt the end of the followup period, 132 (32.9%) of 401 patients had died. Of these, 64 (48.5%) died of cardiovascular disease (CVD). Significant associations between all‐cause mortality and baseline levels of sTNFRI and sTNFRII were identified in men (HR 1.7 [95% CI 1.2–2.4] and HR 1.18 [95% CI 1.05–1.32], respectively) and women (HR 1.33 [95% CI 0.99–1.8] and HR 1.14 [95% CI 1.02–1.28], respectively). Analysis including levels of both sTNFRI and sTNFRII indicated that the sTNFRII level was the best overall predictor of mortality. Multivariate analysis also revealed that the sTNFRII level was a predictor of all‐cause and CVD mortality independently of age, sex, disease duration, C‐reactive protein level, erythrocyte sedimentation rate, rheumatoid factor, nodular disease, modified Health Assessment Questionnaire score, taking CVD drugs, and smoking.ConclusionOur data indicate that serum levels of sTNFR are powerful predictors of mortality in RA. Elevated levels are particularly associated with mortality due to CVD and may be useful for identifying patients at increased risk of premature death.
High titer of anti–phosphatidylserine‐prothrombin complex antibodies in patients with cutaneous polyarteritis nodosa
Authors: Kawakami Tamihiro , Yamazaki Masahide , Mizoguchi Masako , Soma Yoshinao 
Published: November 2007

AbstractObjectiveTo investigate possible correlations between cutaneous polyarteritis nodosa (CPN) and antiphospholipid syndrome–associated antibodies.MethodsSixteen patients were referred with CPN features. To investigate the possible role of antiphospholipid antibodies (aPL) in CPN, we measured serum lupus anticoagulant (LAC), IgG and IgM anticardiolipin (aCL) and anti–phosphatidylserine‐prothrombin complex (anti‐PS/PT) antibodies, and anti–β2‐glycoprotein I–dependent cardiolipin (anti‐β2GPI/CL) antibodies in the 16 CPN patients, 8 microscopic polyangiitis (MPA) patients, 33 systemic lupus erythematosus (SLE) patients, and 23 healthy controls. LAC was determined according to the Subcommittee on Lupus Anticoagulant/Phospholipid Dependent Antibody guidelines. Anti‐PS/PT, aCL, and anti‐β2GPI/CL antibodies were measured by enzyme‐linked immunosorbent assay.ResultsAnti‐PS/PT antibodies and/or LAC were detected in all CPN patients, but not in any controls. Serum IgM anti‐PS/PT antibody was found in 13 (81.3%) CPN patients. The mean ± SD serum anti‐PS/PT IgM level (19.9 ± 12.4 units/ml) in CPN patients was significantly elevated compared with SLE patients (5.7 ± 5.9 units/ml). IgG anti‐PS/PT antibody was detected in 5 (31.3%) CPN patients, but not in any controls. The IgG PS/PT antibody titers were similar in CPN patients (12.3 ± 12.0 units/ml) and SLE patients (13.8 ± 14.3 units/ml). Three (18.8%) CPN patients were positive for IgG aCL antibody and 2 (12.5%) for IgM aCL antibody. No MPA patients had aPL. CPN skin manifestations included livedo reticularis (14 [87.5%]). Direct immunofluorescence (DIF) revealed C3 within the affected vessels in 7 (77.8%) of 9 CPN patients.ConclusionOur study demonstrated that presence of anti‐PS/PT antibodies and/or LAC could serve as markers in CPN patients. CPN could be dependently associated with the presence of anti‐PS/PT antibody. Clinicians need to recognize these titers to permit early accurate diagnosis and treatment. We believe that anti‐PS/PT antibodies will become widely recognized as a new factor when diagnosing CPN.
Reproducibility of the measurement of knee joint proprioception in patients with osteoarthritis of the knee
Authors: Hurkmans E. J. , van der Esch M. , Ostelo R. W. J. G. , Knol D. , Dekker J. , Steultjens M. P. M. 
Published: November 2007

AbstractObjectiveTo estimate the inter‐ and intrarater reliability and agreement of instrumented knee joint proprioception measurement in subjects with knee osteoarthritis (OA) and healthy subjects; to assess the effect of variations in the measurement procedure on agreement parameters.MethodsProprioception was measured by a computer‐controlled knee angular motion‐detecting device in a movement‐detecting task. The angular displacement between the starting position and the position at the instant of movement detection by the subject was recorded. Two raters independently assessed knee joint proprioception. After 14 days the assessment was repeated. Complete data were obtained from 24 subjects with knee OA and 26 healthy subjects. The inter‐ and intrarater reliability coefficients (intraclass correlation coefficients [ICC]) and inter‐ and intrarater agreement measures (standard error of measurement [SEM] and minimal detectable difference [MDD]) were calculated. Additionally, the effect of changing the velocity of angular displacement and applying headphone music during the measurement on the absolute error (i.e., SEM and MDD) was estimated at the second occasion.ResultsInterrater reliability was good in subjects with knee OA and healthy subjects (ICC 0.91 and 0.89, respectively). Interrater agreement was higher in subjects with knee OA than in healthy subjects (SEM 2.13° versus 0.43°, MDD 5.90° versus 1.19°). Intrarater reliability was good in subjects with knee OA and healthy subjects (ICC 0.91 and 0.86, respectively). Intrarater agreement (SEM and MDD) was 2.26° and 6.26° in subjects with knee OA and 0.39° and 1.08° in healthy subjects. The original measurement and the 2 variations in measurement showed comparable measurement errors for subjects with knee OA and healthy subjects.ConclusionIn knee OA subjects and healthy subjects, knee proprioception measurement shows adequate intra‐ and interreliability. However, the absolute measurement error is rather high. Therefore, this measurement has limited value in the assessment of individual subjects, but can be recommended for scientific research in groups of individuals.
Factors associated with restricted mobility outside the home in community‐dwelling adults ages fifty years and older with knee pain: An example of use of the International Classification of Functioning to investigate participation restriction
Authors: Wilkie Ross , Peat George , Thomas Elaine , Croft Peter 
Published: November 2007

AbstractObjectiveTo investigate the links between knee pain characteristics and restricted mobility outside the home, and how these are influenced by mobility‐specific activity limitation, age, sex, socioeconomic status, environmental factors, and comorbidity.MethodsWe conducted a cross‐sectional survey of community‐dwelling adults age ≥50 years. A total of 2,252 responders reporting pain in and around the knee in the last year were eligible. The primary outcome was self‐reported restricted mobility outside the home in the previous 4 weeks (dichotomized as present or absent).ResultsKnee pain severity was strongly associated with restricted mobility outside the home, an association largely mediated by perceived limitation in walking. After adjusting for demographic and socioeconomic characteristics, individual contributions from selected comorbidities, knee pain severity, limitation in walking, and specific environmental factors remained. These environmental factors included perceived need of aids and assistance (adjusted odds ratio [OR] 3.1, 95% confidence interval [95% CI] 2.2–4.4), poor access to public transportation (adjusted OR 2.3, 95% CI 1.4–3.9), and having no access to a car (adjusted OR 1.6, 95% CI 1.1–2.4).ConclusionThere are a range of potential health and social targets that, if addressed, might reduce restricted mobility outside the home in middle‐aged and older individuals with knee pain. Our results suggest that, in addition to treating the knee symptoms, such targets might include comorbidity, walking ability, and environmental barriers such as poor access to public transportation. Moreover, removing environmental barriers may reduce immobility outside the home even in the continued presence of osteoarthritis symptoms and specific activity limitations.
Racial/ethnic disparities in patient‐reported nonsteroidal antiinflammatory drug (NSAID) risk awareness, patient‐doctor NSAID risk communication, and NSAID risk behavior
Authors: Fry Rachel B. , Ray Midge N. , Cobaugh Daniel J. , Weissman Norman W. , Kiefe Catarina I. , Shewchuk Richard M. , Saag Kenneth G. , Curtis Jeffrey R. , Allison Jeroan J. 
Published: November 2007

AbstractObjectiveNonsteroidal antiinflammatory drugs (NSAIDs) are commonly used and frequently lead to serious adverse events. Little is known about NSAID‐related ethnic/racial disparities. We focused on differences in patient NSAID risk awareness, patient‐doctor NSAID risk communication, and NSAID risk‐avoidance behavior.MethodsWe performed a cross‐sectional analysis of survey data from the Alabama NSAID Patient Safety Study. Eligible patients were ≥65 years old and currently taking prescription NSAIDs (Rx NSAIDS). Generalized linear latent and mixed models accounted for nesting of patients within physicians.ResultsOf all 404 participants, 32% were African American and 73% were female. The mean ± SD age was 72.8 ± 7.5 years, and 64% reported an annual household income <$20,000. African American patients were less likely than white patients to recognize any risk associated with over‐the‐counter (OTC) NSAIDs (13.3% versus 29.3%; P = 0.001) and Rx NSAIDs (31.3% versus 49.6%; P = 0.001), report that their doctor discussed possible NSAID‐related gastrointestinal problems (38.0% versus 52.4%; P = 0.007), and take medications to reduce ulcer risk (30.5% versus 50.2%; P = 0.001). Patients with lower income and education reported significantly less risk awareness for OTC and Rx NSAIDs. Racial/ethnic differences persisted after adjusting for multiple confounders.ConclusionIn this community‐based study of low income elderly individuals receiving NSAIDs, we identified important racial/ethnic differences in risk awareness, communication, and behavior. Additional efforts are needed to promote safe NSAID use and reduce ethnic/racial disparities.
Vasculitides associated with malignancies: Analysis of sixty patients
Authors: Fain Olivier , Hamidou Mohamed , Cacoub Patrice , Godeau Bertrand , Wechsler Bertrand , ParIès Jacques , Stirnemann Jérôme , Morin Anne‐Sophie , Gatfosse Marc , Hanslik Thomas , Belmatoug Nadia , Blétry Olivier , Cevallos Ramiro , Delevaux Isabelle , Fisher Evelyne , Hayem Gilles , Kaplan Gérard , Le hello Claire , Mouthon Luc , Larroche Claire , Lemaire Véra , Piette Anne‐Marie , Piette Jean‐Charles , Ponge Thierry , Puechal Xavier , Rossert Jérôme , Sarrot‐Reynauld Françoise , Sicard Didier , Ziza Jean‐Marc , Kahn Marcel‐Francis , Guillevin Loïc 
Published: November 2007

AbstractObjectiveTo describe characteristics and outcomes of vasculitides associated with malignancies.MethodsThe requirement for inclusion in this retrospective, 10‐year study was development of vasculitis in patients with a progressing malignancy. Malignancies secondary to immunosuppressants used to treat vasculitis were excluded. The main characteristics of vasculitides were analyzed and compared according to the type of malignancy.ResultsSixty patients were included (male/female sex ratio 2.53, mean age 62.4 years). Mean followup duration was 45.2 months. Vasculitides were cutaneous leukocytoclastic (45%), polyarteritis nodosa (36.7%), Wegener's granulomatosis (6.7%), microscopic polyangiitis (5%), and Henoch‐Schönlein purpura (5%). Malignancies were distributed as follows: hematologic in 63.1%, myelodysplastic syndrome (MDS) in 32.3%, lymphoid in 29.2%, and solid tumor in 36.9%. Vasculitides were diagnosed concurrently with malignancy in 38% of the cases. Manifestations of vasculitides were fever (41.7%), cutaneous involvement (78.3%), arthralgias (46.7%), peripheral neuropathy (31.7%), renal involvement (23.3%; 11.7% glomerulonephritis, 11.7% microaneurysms, 6.7% renal insufficiency), and antineutrophil cytoplasmic antibody (20.4%). Vasculitis treatments were corticosteroids (78.3%) and immunosuppressant(s) (41.7%). Vasculitis was cured in 65% of patients, but 58.3% died, with 1 death secondary to vasculitis. Independent of subtype, patients with vasculitides associated with MDS more frequently had renal manifestations (P = 0.02) and steroid dependence (P = 0.04) and achieved complete remission less often (P = 0.04) than patients with vasculitides associated with other malignancies. Patients with vasculitides associated with a solid tumor more frequently had peripheral neurologic involvement (P = 0.05). Patients with vasculitides associated with lymphoid malignancy had less frequent arthralgias (P = 0.01) and renal involvement (P = 0.02).ConclusionVasculitides occurring during malignancies present distinctive features according to the vasculitis subtype and nature of the malignancy.
Impact of memory impairment on employment status in persons with systemic lupus erythematosus
Authors: Panopalis Pantelis , Julian Laura , Yazdany Jinoos , Gillis Joann Zell , Trupin Laura , Hersh Aimee , Criswell Lindsey A. , Katz Patricia , Yelin Edward 
Published: November 2007

AbstractObjectiveTo assess the specific contribution of memory impairment to employment status in persons with systemic lupus erythematosus (SLE).MethodsA total of 832 patients with SLE were surveyed and data collected on demographics, SLE symptoms and activity, health status, depression, medications, health resource utilization, and current employment status. Participants underwent screening for memory impairment and based on their scores were categorized to 3 levels of memory function: intact, mild‐moderate impairment, and severe impairment. Employment status was compared across impairment levels using multivariate logistic regression, adjusting for sociodemographic characteristics (i.e., age, sex, race, education, and marital status), employment status at year of diagnosis, disease activity, disease duration, and depression.ResultsIn the intact memory function group, 54.2% were employed, versus 40.6% in the mild‐moderate impairment group and 31.0% in the severe impairment group. In the intact memory function group, 29.2% were unable to work, versus 40.6% in the mild‐moderate impairment group and 56.3% in the severe impairment group. After multivariate adjustment, increasing levels of memory impairment predicted a decreased likelihood of being employed: odds ratio (OR) 0.70, 95% confidence interval (95% CI) 0.48–1.02 for the mild‐moderate impairment group and OR 0.57, 95% CI 0.32–1.00 for the severe impairment group. Participants with memory impairment were more likely to report being unable to work: OR 1.36, 95% CI 0.90–2.04 for the mild‐moderate impairment group, and OR 1.99, 95% CI 1.12–3.55 for the severe impairment group. These findings were statistically significant only in the severe impairment groups.ConclusionThe findings suggest that severe memory impairment is an important factor associated with employment status in persons with SLE.
Prognosis of hip pain in general practice: A prospective followup study
Authors: Lievense Annet M. , Koes Bart W. , Verhaar Jan A. N. , Bohnen Arthur M. , Bierma‐Zeinstra Sita M. A. 
Published: November 2007

AbstractObjectiveHip pain in the elderly is the main feature of osteoarthritis of the hip. In this prospective followup study, we wanted to determine which patients with hip pain show disease progression, and what the incidence of total hip replacement (THR) is in this group of patients after 3 and 6 years of followup.MethodsWithin general practices in the area of Rotterdam (The Netherlands), persons age ≥50 years with incident hip pain were included. After 3 and 6 years, progression of hip pain was assessed.ResultsA total of 224 patients were included. After 3 years disease progressed in 29 (15%) patients and 23 (12%) received a THR. After 6 years disease progressed in 45 (28%) patients and 36 (22%) received a THR. The prognostic variables for a THR after 3 and 6 years related to history taking were age ≥60 years, morning stiffness, and pain in the groin/medial thigh; variables related to physical examination were decreased extension/adduction, painful internal rotation, and a body mass index ≤30 kg/m2; and the variable related to radiologic findings was a Kellgren/Lawrence grade of 2 or higher.ConclusionIn this study population, ∼12% of patients presenting with hip pain to their general practitioner will undergo a THR within 3 years, and ∼22% after 6 years. Using the variables obtained from history taking, physical examination, and radiologic findings enables better identification of persons at high risk for a THR.