bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research tissue banking in otolaryngology: organization, methods and uses, with reference to practical, ethical and legal issues
Authors: Pitchers M , Stokes A , Lonsdale R , Premachandra D J , Edwards D R 
Published: March 2006

The field of molecular biology is an important part of research into neoplastic conditions. Much of this research requires access to human tissue samples, which may need to be collected and stored in a specific way, according to the type of study for which they are intended. Anonymous clinical information about the samples enables researchers to apply results to different patient groups. Access to stored tissues is particularly important in head and neck disease research as many neoplastic conditions affecting this area have a relatively low incidence. Consequently, it may take a long time to build up enough individual cases to make a study worthwhile.We describe here the current legal, ethical and practical issues of research tissue banking, with regard to head and neck disease.
Hearing aid insertion: correlation between patients' confidence and ability
Authors: Pothier D D , Bredenkamp C 
Published: March 2006

Introduction: Once fitted with hearing aids, much reliance is placed on patients' own ability to manage their devices effectively. There has, however, been little research to assess how patients' own confidence compares with their actual ability to manage their hearing aids. This study compares patients' perceptions of their ability to insert their hearing aids with their observed ability to insert the devices satisfactorily.Method: Eighty-five patients provided a rating of their level of confidence in fitting their hearing aid, using a visual analogue score (VAS). This was then compared with their observed level of ability, assessed by an audiologist, also using a VAS (both scores 0–100 mm).Results: We found a weak to moderate correlation between the subjective and objective scores (Pearson r = 0.4912).Conclusions: From these results, we would advise caution when accepting a patient's perceived level of ability as the only indicator of their true ability to insert their hearing aid. This also has important implications for follow-up services that rely only on telephone conversations with patients after a first fitting.
Tracheoesophageal voice prostheses complications in north Glasgow
Authors: Calder N , MacAndie C , MacGregor F 
Published: March 2006

The objectives of this study were to determine (1) the rates of complications, admissions to hospital and requirements for further surgery in patients fitted with tracheoesophageal fistula speech valves, and (2) whether any factors were predictive of complications. A case note review was undertaken of all patients undergoing a laryngectomy at Gartnavel General and Stobhill Hospitals over a 10-year period. One hundred patients were identified.Forty-five patients had complications from their valves, most commonly granulation tissue formation. Thirty-five had a least one admission related to complications and 34 required further surgery. Sixty-seven were vocalizing with the valve.Radiotherapy and valve type were not statistically significant in predicting complications in this study. Primary puncture was associated with a higher rate of complications, although the numbers undergoing secondary puncture were small.
New concepts and techniques in eco-composition
Authors: KELLER DAMIÁN , CAPASSO ARIADNA 
Published: March 2006

This paper has two main objectives: (i) to discuss essential concepts of the ecological approach to composition, proposing the term eco-composition as a common reference for theoretical perspectives and compositional techniques; and (ii) to present the multimedia piece Vivir sin después, realised within the context of the Paititi Project.Following the Introduction, the paper is divided into two main sections. In section 2 we discuss issues raised by the application of the ecological approach to composition. Regarding the data-gathering procedures, eco-composition provides two effective methods: re-enaction and anchoring. Organisation of sonic materials is done through accumulation of heterarchical layers, giving rise to emergent musical phenomena. Section 3 dwells on the compositional strategies applied in the multimedia installation Vivir sin después. We describe the process of re-enacting the first Spanish trip through the Amazon River Basin, carried out by Francisco de Orellana and fifty-nine Spaniards in 1541. Then we address technical aspects of the piece, such as the application of multiple views of single entities, the development of non-hierarchical forms and the manipulation of sonic fields.
Deconstructing Speech: new tools for speech manipulation
Authors: KELLY EDWARD 
Published: March 2006

My research at the London College of Communication is concerned with archives of recorded speech, what new tools need to be devised for its manipulation and how to go about this process of invention. Research into available forms of analysis of speech is discussed below with regard to two specific areas, feature vectors from linear predictive coding (LPC) analysis and hidden Markov-model-based automatic speech recognition (ASR) systems. These are discussed in order to demonstrate that whilst aspects of each may be useful in devising a system of speech-archive manipulation for artistic use. Their drawbacks and deficiencies for use in art – consequent of the reasons for their invention – necessitate the creation of tools with artistic, rather than engineering agendas in mind.It is through the initial process of devising conceptual tools for understanding speech as sound objects that I have been confronted with issues of semiotics and semantics of the voice and of the relationship between sound and meaning in speech, and of the role of analysis in mediating existing methods of communication. This is discussed with reference to Jean-Jacques Nattiez's Music and Discourse: Towards a Semiology of Music (Nattiez 1987). The ‘trace’ – a neutral level of semiotic analysis proposed by Nattiez, far from being hypothetical as suggested by Hatten (1992: 88–98) and others, is present by analogy to many forms of mediation in modern spoken communication and the reproduction of music, and it is precisely this neutrality with regards to meaning that tools for manipulation of speech must possess, since the relationships between the sound of speech and its meaning are ‘intense’ (after Deleuze 1968).
Central and Southern Jordan in the Ayyubid Period: Historical and Archaeological Perspectives
Authors: Milwright Marcus 
Published: March 2006

AbstractFor most of the Islamic period central and southern Jordan has existed on the periphery of larger states. During the twelfth and thirteenth centuries the political and economic life of the Muslim and Frankish polities of Bilad al-Sham (Greater Syria) was dominated by cities like Damascus, Aleppo, Jerusalem and Acre. These urban centres contained the greatest concentrations of the military and civilian elite, and provided the largest markets for locally produced and imported commodities. Intellectual activity also prospered in such urban environments and, with few exceptions, it was the occupants of the cities of Bilad al-Sham who composed the histories of the Crusader states and the Ayyubid confederacy. These chronicles tend to reflect the interests of the ruling elites and generally pay rather less attention to rural areas. The lands between the Wadi Zarqa' and the Red Sea – populated as they were by villages and small market towns – excited little interest among historians, and only Karak and Shawbak merit relatively regular mentions in the chronicles of the period.
Kinetic analysis using low-molecular mass xyloglucan oligosaccharides defines the catalytic mechanism of a Populus xyloglucan endotransglycosylase
Authors: Saura-Valls Marc , Fauré Régis , Ragàs Sergi , Piens Kathleen , Brumer Harry , Teeri Tuula T. , Cottaz Sylvain , Driguez Hugues , Planas Antoni 
Published: March 2006

Plant XETs [XG (xyloglucan) endotransglycosylases] catalyse the transglycosylation from a XG donor to a XG or low-molecular-mass XG fragment as the acceptor, and are thought to be important enzymes in the formation and remodelling of the cellulose-XG three-dimensional network in the primary plant cell wall. Current methods to assay XET activity use the XG polysaccharide as the donor substrate, and present limitations for kinetic and mechanistic studies of XET action due to the polymeric and polydisperse nature of the substrate. A novel activity assay based on HPCE (high performance capillary electrophoresis), in conjunction with a defined low-molecular-mass XGO {XG oligosaccharide; (XXXGXXXG, where G=Glcβ1,4- and X=[Xylα1,6]Glcβ1,4-)} as the glycosyl donor and a heptasaccharide derivatized with ANTS [8-aminonaphthalene-1,3,6-trisulphonic acid; (XXXG-ANTS)] as the acceptor substrate was developed and validated. The recombinant enzyme PttXET16A from Populus tremula x tremuloides (hybrid aspen) was characterized using the donor/acceptor pair indicated above, for which preparative scale syntheses have been optimized. The low-molecular-mass donor underwent a single transglycosylation reaction to the acceptor substrate under initial-rate conditions, with a pH optimum at 5.0 and maximal activity between 30 and 40 °C. Kinetic data are best explained by a ping-pong bi-bi mechanism with substrate inhibition by both donor and acceptor. This is the first assay for XETs using a donor substrate other than polymeric XG, enabling quantitative kinetic analysis of different XGO donors for specificity, and subsite mapping studies of XET enzymes.
Weaning-induced expression of a milk-fat globule protein, MFG-E8, in mouse mammary glands, as demonstrated by the analyses of its mRNA, protein and phosphatidylserine-binding activity
Authors: Nakatani Hajime , Aoki Naohito , Nakagawa Yoshimi , Jin-No Shinji , Aoyama Koji , Oshima Kenji , Ohira Sachiyo , Sato Chihiro , Nadano Daita , Matsuda Tsukasa 
Published: March 2006

A milk membrane glycoprotein, MFG-E8 [milk fat globule-EGF (epidermal growth factor) factor 8], is expressed abundantly in lactating mammary glands in stage- and tissue-specific manners, and has been believed to be secreted in association with milk fat globules. In the present paper, we describe further up-regulation of MFG-E8 in involuting mammary glands, where the glands undergo a substantial increase in the rate of epithelial cell apoptosis, and a possible role of MFG-E8 in mediating recognition and engulfment of apoptotic cells through its specific binding to PS (phosphatidylserine). Immunoblotting and RNA blotting analyses revealed that both MFG-E8 protein and MFG-E8 mRNA were markedly increased in mammary tissue within 3 days of either natural or forced weaning (pup withdrawal) of lactating mice. Using immunohistochemical analysis of the mammary tissue cryosections, the MFG-E8 signal was detected around the epithelium of such involuting mammary glands, but was almost undetectable at early- and mid-lactation stages, although strong signals were obtained for milk fat globules stored in the alveolar lumen. Some signals double positive to a macrophage differentiation marker, CD68, and MFG-E8 were detected in the post-weaning mammary tissue, although such double-positive signals were much smaller in number than the MFG-E8 single-positive ones. Total MFG-E8 in milk was also increased in the post-weaning mammary glands and, furthermore, the free MFG-E8 content in the post-weaning milk, as measured by in vitro PS-binding and apoptotic HC11 cell-binding activities, was much higher than that of lactation. In addition, the post-weaning milk enhanced the binding of apoptotic HC11 cells to J774 macrophages. Sucrose density-gradient ultracentrifugation analyses revealed that such enhanced PS-binding activity of MFG-E8 was present in membrane vesicle fractions (density 1.05–1.13 g/ml), rather than milk fat globule fractions. The weaning-induced MFG-E8 might play an important role in the recognition and engulfment of apoptotic epithelial cells by the neighbouring phagocytic epithelial cells in involuting mammary glands.
Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis
Authors: Stines-Chaumeil Claire , Talfournier François , Branlant Guy 
Published: March 2006

Homotetrameric MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis catalyses the NAD-dependent oxidation of MMSA (methylmalonate semialdehyde) and MSA (malonate semialdehyde) into PPCoA (propionyl-CoA) and acetyl-CoA respectively via a two-step mechanism. In the present study, a detailed mechanistic characterization of the MSDH-catalysed reaction has been carried out. The results suggest that NAD binding elicits a structural imprinting of the apoenzyme, which explains the marked lag-phase observed in the activity assay. The enzyme also exhibits a half-of-the-sites reactivity, with two subunits being active per tetramer. This result correlates well with the presence of two populations of catalytic Cys302 in both the apo- and holo-enzymes. Binding of NAD causes a decrease in reactivity of the two Cys302 residues belonging to the two active subunits and a pKapp shift from approx. 8.8 to 8.0. A study of the rate of acylation as a function of pH revealed a decrease in the pKapp of the two active Cys302 residues to approx. 5.5. Taken to-gether, these results support a sequential Cys302 activation process with a pKapp shift from approx. 8.8 in the apo-form to 8.0 in the binary complex and finally to approx. 5.5 in the ternary complex. The rate-limiting step is associated with the β-decarboxylation process which occurs on the thioacylenzyme intermediate after NADH release and before transthioesterification. These data also indicate that bicarbonate, the formation of which is enzyme-catalysed, is the end-product of the reaction.
Intracellular localization of p40, a protein identified in a preparation of lysosomal membranes
Authors: Boonen Marielle , Hamer Isabelle , Boussac Muriel , Delsaute Anne-Françoise , Flamion Bruno , Garin Jérôme , Jadot Michel 
Published: March 2006

Unlike lysosomal soluble proteins, few lysosomal membrane proteins have been identified. Rat liver lysosomes were purified by centrifugation on a Nycodenz density gradient. The most hydrophobic proteins were extracted from the lysosome membrane preparation and were identified by MS. We focused our attention on a protein of approx. 40 kDa, p40, which contains seven to ten putative transmembrane domains and four lysosomal consensus sorting motifs in its sequence. Knowing that preparations of lysosomes obtained by centrifugation always contain contaminant membranes, we combined biochemical and morphological methods to analyse the subcellular localization of p40. The results of subcellular fractionation of mouse liver homogenates validate the lysosomal residence of p40. In particular, a density shift of lysosomes induced by Triton WR-1339 similarly affected the distributions of p40 and β-galactosidase, a lysosomal marker protein. We confirmed by fluorescence microscopy on eukaryotic cells transfected with p40 or p40–GFP (green fluorescent protein) constructs that p40 is localized in lysosomes. A first molecular characterization of p40 in transfected Cos-7 cells revealed that it is an unglycosylated protein tightly associated with membranes. Taken together, our results strongly support the hypothesis that p40 is an authentic lysosomal membrane protein.
A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes
Authors: Gómez-García María R. , Losada Manuel , Serrano Aurelio 
Published: March 2006

Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are encoded by two different cDNAs, as demonstrated by peptide mass fingerprint analysis. Molecular phylogenetic analyses indicated that Cr-sPPase I is closely related to other eukaryotic sPPases, whereas Cr-sPPase II resembles its prokaryotic counterparts. Chloroplastic sPPase I may have replaced a cyanobacterial ancestor very early during plastid evolution. Cr-sPPase II orthologues are found in members of the green photosynthetic lineage, but not in animals or fungi. These two sPPases from photosynthetic eukaryotes are novel monomeric family I sPPases with different molecular phylogenies and cellular localizations.
Haemoglobin and flow-mediated vasodilation
Authors: Madsen Per Lav , Scheuermann Freestone Michaela , Neubauer Stefan , Channon Keith , Clarke Kieran 
Published: March 2006

A low [Hb] (Hb concentration) is out-balanced by peripheral vasodilation via mechanisms that are incompletely understood. Peripheral vasodilation is influenced by NO (nitric oxide) released from vascular endothelium in response to increased vessel wall shear stress, and absorption by Hb is the main mechanism by which the bioactivity of NO is disarmed. Thus we propose that graded NO absorption is the mechanism through which a low [Hb] is related to peripheral vasodilation. In the present study, we examined the relationship between [Hb] and FMD (flow-mediated vasodilation; 5 min of cuff ischaemia) of the radial and brachial arteries in 33 normal subjects and in 13 patients with Type II diabetes, known to have impaired NO-mediated vasodilation. The smaller radial artery provided the more sensitive test, as it had a 2-fold larger FMD than the brachial artery (22±18% compared with 9±18% respectively, in normal subjects; means±S.D., P&lt;0.05). FMD of the radial artery had a negative correlation with [Hb] (r2=−0.66, P&lt;0.05; n=27). In subjects with [Hb] below and above the median of 14.1 g/dl, the radial artery FMD was 30±22% compared with 13±12% respectively (P&lt;0.05). In diabetic patients, FMD was lower and a co-variation with [Hb] could not be established. Thus, in normal subjects, NO-mediated endothelium-related vasodilation at least partly out-balanced the ‘added burden’ of a low [Hb] during post-ischaemic reperfusion.
Detection of Postoperative Surgical-Site Infections: Comparison of Health Plan–Based Surveillance With Hospital-Based Programs
Authors: Sands Kenneth E. , Yokoe Deborah S. , Hooper David C. , Tully John L. , Horan Teresa C. , Gaynes Robert P. , Solomon Steven L. , Platt Richard 
Published: March 2006

AbstractBackground:Review of health plan administrative data has been shown to be more sensitive than other methods for identifying postdischarge surgical-site infections (SSIs), but there has not been a direct comparison between this method and hospital-based surveillance for all infections, including those diagnosed before discharge. We compared these two methods for identifying SSIs following coronary artery bypass graft (CABG) procedures:.Methods:We studied 1,352 CABG procedures performed among members of one health plan from March 1993 through June 1997. Health plan administrative records were reviewed based on claims containing diagnoses or procedures suggestive of infection or outpatient dispensing of antibiotics appropriate for SSI. Hospital-based surveillance information was also reviewed. SSI rates were calculated based on the total events identified by either mechanism.Results:Postdischarge information was reviewed for 328 (85%) of 388 procedures. SSIs were confirmed in 167 patients (13% overall risk of confirmed SSI; range, 3% to 14% in the 5 hospitals). The overall sensitivity of hospital-based surveillance was 49.7% (83 of 167), and that of health plan data was 71.8% (120 of 167). There was no significant difference among hospitals in the sensitivity of either surveillance mechanism.Conclusions:Surveillance based on health plan data identified more postoperative infections, including those occurring before discharge, than did hospital-based surveillance. Screening administrative data and pharmacy activity may be an important adjunct to SSI surveillance, allowing efficient comparison of hospital-specific rates. Interpretation of differences among hospitals' infection rates requires case mix adjustment and understanding of variations in hospitals' discharge diagnosis coding practices
Standardized Infection Ratios for Three General Surgery Procedures: A Comparison Between Spanish Hospitals and U.S. Centers Participating in the National Nosocomial Infections Surveillance System
Authors: Jodra V. Monge , Rodela A. Robustillo , Martínez F. Martín , Fresneña N. López ,   Quality Control Indicator Working Group
Published: March 2006

AbstractObjectives:To compare Spanish surgical wound infection (SWI) rates for three procedures with those published by the U.S. NNIS System, and to analyze quarterly trends.Design:This was a 4-year prospective analysis of SWI using data from a Spanish nosocomial infection surveillance network based on CDC classification criteria. SWI rates were computed as standardized infection ratios (SIRs). Trends for both SWIs and SIRs were evaluated by linear regression.Setting:Forty-three Spanish hospitals during 1997 through 2000.Patients:Those undergoing cholecystectomy (n = 7,631), appendectomy (n = 5,780), and herniorrhaphy (n = 9,864).Results:For cholecystectomy patients, the SWI rate was 4.38% and the SIR was 3.32. Both of these variables showed a slightly rising, although nonsignificant, linear trend during the study period. For appendectomy patients, the SWI rate was 7.94% and the SIR was 2.86. The linear trend was increasing for both, but only the SWI rate attained significance. For herniorrhaphy patients, the SWI rate was 1.77% and the SIR was 1.64. Both of these variables showed a significant descending tendency during the 4 years.Conclusions:Because the SIR takes into account the patient risk category, it is the best indicator of the trend shown by the SWI rate over time for a given surgical procedure. According to our comparison of SIRs with reference NNIS System values, SWI rates for cholecystectomy and appendectomy were high. Monitoring of the SIR will provide a basis for the design of infection control measures and the assessment of their effectiveness.
PseudomonasSurgical-Site Infections Linked to a Healthcare Worker With Onychomycosis
Authors: Mermel Leonard A. , McKay Maria , Dempsey Jane , Parenteau Stephen 
Published: March 2006

AbstractObjective:To determine the etiology ofPseudomonas aeruginosasurgical-site infections following cardiac surgery.Setting:University teaching hospital.Patients:Those with wound cultures that grewP. aeruginosaafter cardiac surgery performed from 1999 to 2001.Methods:Medical records and operating room (OR) records of patients withP. aeruginosacardiac surgical-site infections from 1999 to 2001 were reviewed. Healthcare workers involved with two or more cases were interviewed and examined. Specimens for environmental cultures were obtained from the ORs and cardiac surgical equipment. Cardiac surgery cases were observed and postoperative care and the cleaning of surgical instruments were investigated. OR air handling system records during the epidemic period were reviewed. Molecular fingerprinting of availableP. aeruginosaisolates from infected patients and a healthcare worker was done.Results:There were fiveP. aeruginosacardiac surgical-site infections from January to August 2001, compared with no such infections from 1999 to 2000. All were adult patients. One cardiac surgeon with onychomycosis operated on all five cases. He did not routinely double glove. The involved fingernail grewP. aeruginosa.ThreeP. aeruginosapatient isolates were available for pulsed-field gel electrophoresis; two were identical to the isolate from the involved surgeon's onychomycotic nail. No environmental OR cultures grewP. aeruginosa.The surgeon's culture-positive nail was completely removed. There have been noP. aeruginosasurgical-site infections among cardiac surgery patients since this intervention.Conclusion:At least two cases of a cluster ofP. aeruginosasurgical-site infections resulted from colonization of a cardiac surgeon's onychomycotic nail.