bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of proteomic profiles of serum, plasma, and modified media supplements used for cell culture and expansion
Authors: Ayache Saleh , Panelli Monica C , Byrne Karen M , Slezak Stefanie , Leitman Susan F , Marincola Francesco M , Stroncek David F 
Published: October 2006

Abstract

Background
The culture and expansion of human cells for clinical use requires the presence of human serum or plasma in culture media. Although these supplements have been extensively characterized in their chemical composition, only recently it has been possible to provide by high throughput protein analysis, a comprehensive profile of the soluble factors contributing to cell survival. This study analyzed and compared the presence of 100 proteins including chemokines, cytokines and soluble factors in six different types of media supplements: serum, plasma, recalcified plasma, heat inactivated serum, heat inactivated plasma and heat inactivated recalcified plasma.

Methods
Serum, plasma, recalcified plasma, and heat inactivated supplements were prepared from ten healthy subjects. The levels of 100 soluble factors were measured in each sample using a multiplexed ELISA assay and compared by Eisen hierarchical clustering analysis.

Results
A comparison of serum and plasma levels of soluble factors found that 2 were greater in plasma but 18 factors were greater in serum including 11 chemokines. The levels of only four factors differed between recalcified plasma and plasma. Heat inactivation had the greatest effect on soluble factors. Supervised Eisen hierarchical clustering indicated that the differences between heat inactivated supplements and those that were not were greater than the differences within these two groups. The levels of 36 factors differed between heat inactivated plasma and plasma. Thirty one of these factors had a lower concentration in heat inactivated plasma including 12 chemokines, 4 growth factors, 4 matrix metalloproteases, and 3 adhesion molecules. Heat inactivated decalcified plasma is often used in place of heat inactivated serum and the levels of 19 soluble factors differed between these two supplements.

Conclusion
Our report provides a comprehensive protein profile of serum, plasma recalcified plasma, and heat inactivated supplements. This profile represents a qualitative and quantitative database that can aid in the selection of the appropriate blood derived supplement for human cell cultures with special requirements.
Functional Studies of Akt Isoform Specificity in Skeletal Muscle in Vivo; Maintained Insulin Sensitivity Despite Reduced Insulin Receptor Substrate-1 Expression
Authors: Cleasby Mark E. , Reinten Tracie A. , Cooney Gregory J. , James David E. , Kraegen Edward W. 
Published: October 2006

AbstractThe phosphoinositide 3-kinase/Akt pathway is thought to be essential for normal insulin action and glucose metabolism in skeletal muscle and has been shown to be dysregulated in insulin resistance. However, the specific roles of and signaling pathways triggered by Akt isoforms have not been fully assessed in muscle in vivo. We overexpressed constitutively active (ca-) Akt-1 or Akt-2 constructs in muscle using in vivo electrotransfer and, after 1 wk, assessed the roles of each isoform on glucose metabolism and fiber growth. We achieved greater than 2.5-fold increases in total Ser473 phosphorylation in muscles expressing ca-Akt-1 and ca-Akt-2, respectively. Both isoforms caused hypertrophy of muscle fibers, consistent with increases in p70S6kinase phosphorylation, and a 60% increase in glycogen accumulation, although only Akt-1 increased glycogen synthase kinase-3β phosphorylation. Akt-2, but not Akt-1, increased basal glucose uptake (by 33%, P = 0.004) and incorporation into glycogen and lipids, suggesting a specific effect on glucose transport. Consistent with this, short hairpin RNA-mediated silencing of Akt-2 caused reductions in glycogen storage and glucose uptake. Consistent with Akt-mediated insulin receptor substrate 1 (IRS-1) degradation, we observed approximately 30% reductions in IRS-1 protein in muscle overexpressing ca-Akt-1 or ca-Akt-2. Despite this, we observed no decrease in insulin-stimulated glucose uptake. Furthermore, a 68% reduction in IRS-1 levels induced using short hairpin RNAs targeting IRS-1 also did not affect glucose disposal after a glucose load. These data indicate distinct roles for Akt-1 and Akt-2 in muscle glucose metabolism and that moderate reductions in IRS-1 expression do not result in the development of insulin resistance in skeletal muscle in vivo.
Foraging energetics of a nectar-feeding ant: metabolic expenditure as a function of food-source profitability
Authors: Schilman Pablo E. , Roces Flavio 
Published: October 2006

SUMMARY
We examined the quantitative relationship between the energetic costs and benefits of nectar collection by nectar-feeding ants, Camponotus rufipes. In the laboratory, individual workers were trained to visit an artificial feeder that provided a sucrose solution of 1%, 5%, 10%, 30% or 50%at controlled flows, in a similar span range to those observed in natural nectar sources. We measured foraging times, nectar loads collected, and CO2 production during actual feeding, as an indication of the energy expenditure for a single forager. Results show an increase in individual metabolic rates with increasing flow rate of sugar solution, but no dependence on sucrose concentration. This increase in metabolic expenditure does not depend on the crop load attained while feeding, as intuitively expected, and is therefore a result of an increased activity brought about by the food-source profitability experienced by the forager. The energy gained during collection of sugar solution is always higher than the energy spent by the ant. Even with a food source of lower quality than a natural source, the ants gain ca. tenfold of what they spend. Based on a simplified model, we calculated that foragers of C. rufipes could travel from 0.5 to 9 km with the energy gained in a single foraging trip only. These results suggest that decreasing foraging time is more important than increasing individual energetic efficiency when workers of the nectar-feeding ant C. rufipes decide to stop drinking and return to the nest with partial crop loads.
Orbital‐facial complications of sinusitis in children
Authors: Fearon Blair , Edmonds Brodie , Bird Russel 
Published: October 2006

AbstractAlthough complications of sinusitis in children have lessened remarkably since the advent of antibiotics, nevertheless they still occur. In a 25‐year period at the Hospital for Sick Children, 6,770 patients with the diagnosis of sinusitis were reviewed and 159 of these had orbital‐facial complications. Surgical intervention was necessary in 17 to prevent more serious complications. Although there were no deaths in non‐malignant disease, two patients developed permanent optic atrophy, presumably because of delayed surgical drainage. On occasion, malignant disease may simulate inflammatory processes.
Tongue strength: Part I: Following total laryngectomy
Authors: Dworkin James P. , Hartman David E. , Keith Robert L. 
Published: October 2006

AbstractSurgical repositioning of the genioglossus muscle, as in total laryngectomy, may have an adverse effect on the protrusive strength of the tongue. To test this premise, anterior and lateral tongue strength measures were obtaind from 10 laryngectomized and 15 normal subjects by means of a pressure transducer and recording device. The results indicated no significant difference between laryngectomized and normal subjects on tongue strength measures. Further, all laryngectomized subjects were judged to have good to excellent esophageal speech. It was concluded that laryngectomy does not adversely affect the major musculature concerned with protrusive tongue strength for proficient esophageal speakers.
Positive delphian lymph node: Clinical significance in laryngeal cancer
Authors: Olsen Kerry D. , Desanto Lawrence W. , Pearson Bruce W. 
Published: October 2006

AbstractThe significance of metastatic cancer in the Delphian lymph node in patients with laryngeal carcinoma has not been thoroughly discussed. Between 1960 and 1985, we identified 20 cases of histologically proven metastasis to the Delphian node. In 12 of these patients with glottic cancer (T1‐T3), the neck was clinically negative but a positive Delphian node was discovered at partial or total laryngectomy; in 6 patients, ipsilateral neck metastasis developed. Eleven of the 20 patients have died from their laryngeal cancer. The frequency of neck metastasis or death, or both, from cancer is unusually high in patients with a positive Delphian node.
Continuous Medicaid Eligibility During the COVID-19 Pandemic and Postpartum Coverage, Health Care, and Outcomes
Authors: Daw Jamie R. , MacCallum-Bridges Colleen L. , Kozhimannil Katy B. , Admon Lindsay K. 
Published: March 2024

ImportancePursuant to the Families First Coronavirus Response Act (FFCRA), continuous Medicaid eligibility during the COVID-19 public health emergency (PHE) created a de facto national extension of pregnancy Medicaid eligibility beyond 60 days postpartum.ObjectiveTo evaluate the association of continuous Medicaid eligibility with postpartum health insurance, health care use, breastfeeding, and depressive symptoms.Design, Setting, and ParticipantsThis cohort study using a generalized difference-in-differences design included 21 states with continuous prepolicy (2017-2019) and postpolicy (2020-2021) participation in the Pregnancy Risk Assessment Monitoring System (PRAMS).ExposuresState-level change in Medicaid income eligibility after 60 days postpartum associated with the FFCRA measured as a percent of the federal poverty level (FPL; ie, the difference in 2020 income eligibility thresholds for pregnant people and low-income adults/parents).Main Outcomes and MeasuresHealth insurance, postpartum visit attendance, contraceptive use (any effective method; long-acting reversible contraceptives), any breastfeeding and depressive symptoms at the time of the PRAMS survey (mean [SD], 4 [1.3] months postpartum).ResultsThe sample included 47 716 PRAMS respondents (64.4% aged &amp;lt;30 years; 18.9% Hispanic, 26.2% non-Hispanic Black, 36.3% non-Hispanic White, and 18.6% other race or ethnicity) with a Medicaid-paid birth. Based on adjusted estimates, a 100% FPL increase in postpartum Medicaid eligibility was associated with a 5.1 percentage point (pp) increase in reported postpartum Medicaid enrollment, no change in commercial coverage, and a 6.6 pp decline in uninsurance. This represents a 40% reduction in postpartum uninsurance after a Medicaid-paid birth compared with the prepolicy baseline of 16.7%. In subgroup analyses by race and ethnicity, uninsurance reductions were observed only among White and Black non-Hispanic individuals; Hispanic individuals had no change. No policy-associated changes were observed in other outcomes.Conclusions and RelevanceIn this cohort study, continuous Medicaid eligibility during the COVID-19 PHE was associated with significantly reduced postpartum uninsurance for people with Medicaid-paid births, but was not associated with postpartum visit attendance, contraception use, breastfeeding, or depressive symptoms at approximately 4 months postpartum. These findings, though limited to the context of the COVID-19 PHE, may offer preliminary insight regarding the potential impact of post-pandemic postpartum Medicaid eligibility extensions. Collection of longer-term and more comprehensive follow-up data on postpartum health care and health will be critical to evaluating the effect of ongoing postpartum policy interventions.
Perspectives of Black Patients on Racism Within Emergency Care
Authors: Agarwal Anish K. , Gonzales Rachel E. , Sagan Charlotte , Nijim Sally , Asch David A. , Merchant Raina M. , South Eugenia C. 
Published: March 2024

ImportanceNumerous Black individuals experience racism persistently throughout their lives, with repercussions extending into health care settings. The perspectives of Black individuals regarding emergency department (ED) care, racism, and patient-centered approaches for dismantling structural racism remain less explored.ObjectiveTo qualitatively explore the perspectives and experiences of Black patients related to race, racism, and health care following a recent ED visit.Design, Setting, and ParticipantsIn this qualitative study, the audio from semistructured interviews of Black patients discharged from an academic urban ED between August 2021 to April 2022 were recorded, transcribed, and analyzed using thematic analysis.Main Outcomes and MeasuresThe main outcomes encompassed the main themes from the analysis of the interviews with Black patients regarding their perspectives on race, racism, and clinical care.ResultsA total of 25 Black patients (20 [80%] female; mean [SD] age, 44.6 [12.9] years) discharged from the ED were interviewed. Three broad domains were identified: (1) racism in health care; (2) ED clinical care; and (3) recommendations for improvement. Within these domains, the first 2 were grouped into specific themes. Within the first domain, racism in health care, 7 themes were identified using thematic analysis: (1) a history of medical racism; (2) dismissiveness; (3) patient expectations on encountering racism; (4) medical mistrust; (5) health literacy; (6) postencounter outcomes, and (7) discrimination beyond but associated with race. Within the second theme, ED clinical care, 5 themes were identified using the same thematic analysis method: (1) discharge plan; (2) patient experience; (3) waiting room perceptions; (4) medication treatment; and (5) pain management. The third domain, recommendations for improvement, incorporated patient-generated suggestions for enhancing the Black patient experience.Conclusions and RelevanceIn this qualitative study, the fabric of clinical care delivery in the ED was intricately woven with Black patients' experiences of racism. Patients expressed a pervasive sense of mistrust, skepticism, and dismissiveness at the system level. Instances of racism were consistently highlighted by patients from their entry to the ED to discharge. These perspectives illuminate the pervasive nature of racism in clinical care, providing valuable insights for exploring patient-centered approaches to foster antiracist cultures in the ED and throughout the broader medical landscape.
Predictive Tool Use and Willingness for Surgery in Patients With Knee Osteoarthritis
Authors: Zhou Yushy , Patten Lauren , Spelman Tim , Bunzli Samantha , Choong Peter F. M. , Dowsey Michelle M. , Schilling Chris 
Published: March 2024

ImportanceDespite the increasing number of tools available to predict the outcomes of total knee arthroplasty (TKA), the effect of these predictive tools on patient decision-making remains uncertain.ObjectiveTo assess the effect of an online predictive tool on patient-reported willingness to undergo TKA.Design, Setting, and ParticipantsThis parallel, double-masked, 2-arm randomized clinical trial compared predictive tool use with treatment as usual (TAU). The study was conducted between June 30, 2022, and July 31, 2023. Participants were followed up for 6 months after enrollment. Participants were recruited from a major Australian private health insurance company and from the surgical waiting list for publicly funded TKA at a tertiary hospital. Eligible participants had unilateral knee osteoarthritis, were contemplating TKA, and had previously tried nonsurgical interventions, such as lifestyle modifications, physiotherapy, and pain medications.InterventionThe intervention group was provided access to an online predictive tool at the beginning of the study. This tool offered information regarding the likelihood of improvement in quality of life if patients chose to undergo TKA. The predictions were based on the patient’s age, sex, and baseline symptoms. Conversely, the control group received TAU without access to the predictive tool.Main Outcomes and MeasuresThe primary outcome measure was the reduction in participants’ willingness to undergo surgery at 6 months after tool use as measured by binomial logistic regression. Secondary outcome measures included participant treatment preference and the quality of their decision-making process as measured by the Knee Decision Quality Instrument.ResultsOf 211 randomized participants (mean [SD] age, 65.8 [8.3] years; 118 female [55.9%]), 105 were allocated to the predictive tool group and 106 to the TAU group. After adjusting for baseline differences in willingness for surgery, the predictive tool did not significantly reduce the primary outcome of willingness for surgery at 6 months (adjusted odds ratio, 0.85; 95% CI, 0.42-1.71; P = .64).Conclusions and RelevanceDespite the absence of treatment effect on willingness for TKA, predictive tools might still enhance health outcomes of patients with knee osteoarthritis. Additional research is needed to optimize the design and implementation of predictive tools, address limitations, and fully understand their effect on the decision-making process in TKA.Trial RegistrationANZCTR.org.au Identifier: ACTRN12622000072718
Anisotropic Fluorinated‐Elastomer‐Blended Micro‐Dominoes for Wearable Triboelectric Nanogenerators
Authors: Lee Giwon , Lee Siyoung , Kim Daegun , Kim Su Hyun , Choi Chungryong , Lee Seung Goo , Cho Kilwon 
Published: March 2024

AbstractTriboelectric nanogenerators (TENGs) have emerged as promising portable and sustainable energy sources in daily life, harvesting energy from human motion, water, and wind. However, they still face limitations in aspects such as contact area, deformability, wettability, and manufacturing method. Here, a wearable TENG incorporating an anisotropic domino structure based on a fluorinated elastomer blend is presented. Because of its thin, elongated structure with broad sides, the TENG achieves substantially larger contact areas and high bendability. Introducing a fluorinated elastomer into the polydimethylsiloxane matrix via a simple blending process not only enhances the triboelectric performance but also reduces surface energy and improves the stretchability of elastomers. The anisotropic arrangement of dominoes, in synergy with the fluorinated elastomer, mimics the surface physicochemical properties of natural rice leaves, resulting in anisotropic superhydrophobic wetting behavior with a self‐cleaning effect and controlled directional water flow for efficient water energy harvesting. Therefore, the TENG functions as an energy‐harvesting leaf that captures energy from wind and water droplets, as well as a wearable energy‐harvesting wristband that generates power from human motions such as touching, shaking, and hand washing.
Competitively Assembled Aramid‐MXene Janus Aerogel Film Exhibiting Concurrently Robust Shielding and Effective Anti‐Reflection Performance
Authors: Xu Jian , Fang Jiangyu , Zuo Peiyuan , Wang Yizhe , Zhuang Qixin 
Published: March 2024

AbstractThe fast progress in electronic devices demands superior anti‐reflective performance of electromagnetic interference shielding materials. However, high‐performance shielding strategies are mainly dependent on reflection effects and this inevitably results in a trade‐off between shielding and anti‐reflective properties during structural designs. Herein, this work proposes a novel gradual heterogeneity design to fundamentally overcome the contradiction. The different steric hindrances in MXene nanosheets and aramid nanoclusters endow the competitively assembled laminal aerogel films with a unique Janus architecture. This Janus structure presents continuous gradient asymmetries and abundant cavities that enable prolonged energy attenuation paths and minimized reflection. Comparing to isotropic counterparts, the gradient design performances a 2174% reduction in reflection coefficient (0.0039) and a 53% increase in shielding efficiency (60.49 dB) in the terahertz band, both of which significantly outperform most state‐of‐the‐art anti‐reflective shielding structures. Moreover, the Janus architecture comprising interconnected arch‐shaped substructures provides the aerogel films with anisotropic EMI and thermal management capabilities, coupled with robust structural and performance stability. This work provides a conceptually novel platform for developing asymmetric architecture for advanced shielding materials.
Demonstration of Two Multi‐Component Target Ablation Approaches and Their Application in Combinatorial Pulsed Laser Deposition
Authors: Jörns A. , von Wenckstern H. , Vogt S. , Schlupp P. , Grundmann M. 
Published: March 2024

AbstractCombinatorial pulsed laser deposition (C‐PLD) based on segmented targets has led to new possibilities in the pace of discovery of novel advanced materials with significantly reduced deposition time and material consumption. However, fabrication of established segmented targets may be complex or not even possible for certain material combinations. In this article, two alternative C‐PLD techniques based on easy‐to‐fabricate segmented targets are presented. One approach uses two semi‐circular segments A and B with a systematic adjustable lateral shift of the target rotation axis from the target center. The other approach is based on a new target design defined by an ABA‐segmentation where B is a horizontal bar between two semi‐circular segments of A. Both approaches enable growth of discrete composition libraries. The concepts and important parameters are introduced and computer simulations as function of the geometric parameters are made to yield the expected thin film compositions. As proof‐of‐concept, the techniques are employed on the transparent, semiconducting ternary alloy zinc‐tin‐oxide. The simulations are in very good agreement with the experimental data. Physical properties of films grown by the demonstrated approaches are compared with those obtained by established PLD processes.
Does corporate social responsibility spending affect cash ratio: Evidence from central public sector enterprises
Authors: Khatri Preeti , Bhatia Mani 
Published: March 2024

AbstractThe researcher examines the effect of spending on corporate social activities on cash holdings. The financial dataset was collected using the Prowess database and corporate social responsibility expenditure from the Indian CSR portal. Using a panel dataset of 12 Central Public Sector Enterprises indexed in the National Stock Exchange was considered as a sample for the current research. A random effect model was applied to test the hypothesis. The study's findings provide a positive indication of the causal effect of spending in corporate social activities on cash holdings. The author has considered the expenditure amount of corporate social responsibility on the sample of public sectors undertaking of India. As in previous studies, authors had considered CSR ratings. This study provides proficient information to the top management level, shareholders, and stakeholders of the Central Public Sector Enterprises regarding the disbursement of corporate social activities in India.