bluuebunny/crossref_metadata_2025_split huggingface dataset:
An immunofluorescent method using crithidia luciliae to detect antibodies to double‐stranded DNA
Authors: Crowe William , Kushner Irving 
Published: February 2007

AbstractThe immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double‐stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug‐induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics of antibodies to dsDNA.
Aseptic necrosis presenting as wrist pain in sle
Authors: Urman Jeffrey D. , Abeles Micha , Houghton Alan N. , Rothfield Naomi F. 
Published: February 2007

AbstractAseptic necrosis of the wrist in systemic lupus erythematosus (SLE) is not rare. In 156 patients with SLE, aseptic necrosis occurred in 11 patients, of whom 3 (27%) had wrist involvement. Onset of pain was insidious and the symptoms were thought to be related to synovitis due to SLE. An average of 11 months elapsed before aseptic necrosis was correctly diagnosed. An awareness of this possibility is important in the management of any patient with SLE who complains of wrist pain.
Antigen‐induced arthritis in mice
Authors: Brackertz Dieter , Mitchell Graham F. , Mackay Ian R. 
Published: February 2007

AbstractAntigen‐induced arthritis was established in the mouse by immunization with methylated bovine serum albumin (mBSA) in complete Freund's adjuvant with B pertussis vaccine. The knee joint was injected after 21 days with mBSA in saline. The arthritis was chronic, antigen‐specific, and T‐cell dependent in hypothymic nu/nu mice. C57BL and BALB/c mice were susceptible, whereas CBA mice were relatively resistant. Susceptibility was dominant; one gene was loosely linked to the “b” allele of the H‐2 complex of C57BL mice.
Decreased lymphocyte reactivity to a suboptimal concentration of phytohemagglutinin in sjögren's syndrome
Authors: Michalski Joseph P. , Mccombs Candace 
Published: February 2007

AbstractSignificantly decreased lymphocyte reactivity to a suboptimal concentration of phytohemagglutinin (PHA) was found in patients with Sjögren's syndrome (SS), whereas the response to an optimal concentration was generally normal. Kinetic studies were performed on control lymphocytes. Only suboptimal PHA concentrations stimulated tritiated thymidine (3H‐TdR) incorporation in proportion to the number of potentially reactive lymphocytes. Kinetic studies of SS patients suggested that their low reactivity was attributable to fewer functionally reactive lymphocytes rather than to a decreased rate of proliferation.
Connective tissue activation
Authors: Castor C. W. , Ritchie J. C. , Scott M. E. , Whitney S. L. 
Published: February 2007

AbstractThis report describes a small basic protein found in human platelets which stimulates hyaluronic acid formation, glucose uptake, and lactate formation in several types of human connective tissue cells. In addition, it stimulates the incorporation of [3H] methyl thymidine into DNA in human synovial cell cultures. This platelet factor (connective tissue activating peptide‐III, CTAP‐III) clearly differs from CTAP‐I, found in human lymphocytes, and may play a role as a mediator of the inflammatory process.
Chronic polyarthritis associated with hypogammaglobulinemia
Authors: Grayzel Arthur I. , Marcus Robert , Stern Richard , Winchester Robert J. 
Published: February 2007

AbstractTwo adult patients with hypogammaglobulinemia and chronic synovitis were studied. Synovial tissue and fluid were examined by histologic, virologic, and immunologic methods. The synovium lacked characteristic histologic features of rheumatoid arthritis, and B lymphocytes were absent from the synovial tissue, fluid, and peripheral blood. These 2 patients may represent a form of chronic synovitis produced in the absence of any B lymphocyte response.
Comparison of clinical features in HLA‐B27 positive and negative patients with ankylosing spondylitis
Authors: Khan Muhammad A. , Kushner Irving , Braun William E. 
Published: February 2007

AbstractThe clinical features of ankylosing spondylitis (AS) were compared in 63 HLA‐B 27 positive (+) and 15 B27 negative (−) individuals with this disease. There were no differences in age at onset, functional class, degree of deformity, pain, severity of X‐ray changes, or frequency of peripheral joint involvement or of reconstructive orthopedic surgery. These data demonstrated that skeletal manifestations of AS were essentially the same in B27(+) and (−) patients, and provide no evidence for the speculation that AS in B27(−) patients is milder or is a different disease from that occurring in B27(+) patients. On the other hand, acute anterior uveitis was found to be significantly more common in B27(+) patients, a fact suggesting that the “uveitis of AS” may in fact be an independent condition occurring in B27(+) individuals, rather than a manifestation of AS per se.
Disseminated immune deposits in lupus erythematosus
Authors: Brentjens Jan , Ossi Elena , Albini Boris , Sepulveda Marion , Kano Kyoichi , Sheffer John , Vasilion Peter , Marine Edward , Baliah Tadla , Jockin Hubert , Andres Giuseppe 
Published: February 2007

AbstractImmunohistologic studies were performed on autopsy tissues of 2 patients with systemic lupus erythematosus. All tissues examined—the kidney, lung, spleen, liver, intestine, peritoneum, and choroid plexus—contained immune deposits. Antinuclear antibody concentration in immunoglobulin G eluted from lung and spleen tissue was higher than in serum immunoglobulin G. These findings support the assumption that in systemic lupus erythematosus the renal as well as the extrarenal lesions can be attributed to vascular deposition of immune complexes.
Digital mucinous pseudocysts
Authors: Goldman John A. , Goldman Leon , Jaffe Murray S. , Richfield Daniel F. 
Published: February 2007

AbstractThirty‐one patients with 46 digital mucinous pseudocysts are reviewed. The pseudocysts are usually soft, fluctuant, dome‐shaped, dimpled, smooth lesions located near and often associated with a linear defect of the fingernail. Transillumination confirms the diagnosis. Treatment is conservative, usually consisting of intralesional injections. If surgery is necessary, distal interphalangeal joint injection of methylene blue aids in complete removal by identifying the mesothelial‐lined pedicle leading from the distal joint to the pseudocyst.
Industrial rheumatology
Authors: Hadler Nortin M. 
Published: February 2007

AbstractRegional musculoskeletal diseases are exceedingly common in all segments of society, and dramatically so in industry. For generations, medicine has assumed that many such entities are use‐associated. A critical review of several examples of back and upper extremity disease demonstrates that the literature supporting these assumptions is almost entirely anecdotal. It is argued that if defined patterns of usage were associated with defined clinical syndromes, such knowledge would have considerable therapeutic and prophylactic potential. Industrial rheumatology is proposed as an investigative discipline for the study of such relationships. The prerequisites in terms of assumptions and methods as well as potential fallacies and pitfalls are discussed.
Bone Marrow Transplantation in Patients with Gold‐Induced Marrow Aplasia
Authors: Baldwin John L. , Storb Rainer , Thomas E. Donnall , Mannik Mart 
Published: February 2007

AbstractAplastic anemia is a rare but usually fatal complication of gold salt therapy for rheumatoid arthritis. This report describes 3 patients who developed aplastic anemia while receiving gold salts, and a fourth patient who developed aplastic anemia after receiving gold and then cytotoxic agents. These patients failed to respond to conventional therapy for aplastic anemia and subsequently received bone marrow transplants from HLA‐matched siblings. Engraftment occurred in all 4 patients. One patient is alive 2 years after transplantation, and 3 patients died of complications following transplantation.
Cathepsin D Agglutinators in Rheumatoid Arthritis
Authors: Artmann G. , Fehr K. , Böni A. 
Published: February 2007

AbstractThe incidence and titer of cathepsin D agglutinators (CDA) were significantly higher in seropositive rheumatoid arthritis (RA) sera than in the sera of healthy blood donors or of patients with other rheumatic diseases, including seronegative RA. A significant elevation was also found in synovial fluid (SF) samples from seropositive RA patients. CDA in the SF tended to be increased when compared with the corresponding serum. The levels of CDA correlated positively with those of rheumatoid factors (RF) when the latter were determined with IgG anti‐CD Ripley‐coated erythrocytes, but not when they were determined by the Waaler‐Rose or latex tests. The increased CDA titers do not seem to be the result of significant amounts of IgM agglutinators or of the presence of IgM‐RF. These findings suggest the existence of a link between the formation of RF and CDA, but the nature of this link cannot be fully explained.
Thoracic Duct Drainage in Sle with Cutaneous Vasculitis
Authors: Nyman Kenneth E. , Bangert Ruth , Machleder Herbert , Paulus Harold E. 
Published: February 2007

AbstractA 25‐year‐old woman had systemic lupus erythematosus manifested primarily by severe cutaneous vasculitis that was unresponsive to oral prednisone and azathioprine. She was treated with thoracic duct drainage (TDD). Her course was followed by serial photographs, skin biopsies, and serum immunoglobulin, antinuclear antibody, and complement levels. After I week of drainage there was obvious clinical improvement. During the remainder of her drainage, the vasculitis continued to improve. It was possible to taper oral corticosteroids and to discontinue azathioprine during TDD without any clinical or laboratory evidence of exacerbation. The patient had no recurrence of vasculitis 22 weeks after the termination of TDD.
T‐Lymphocyte Subpopulation in Untreated SLE
Authors: Rivero Santiago J. , Llorente Luis , Díaz‐Jouanen Efraín , Alarcón‐Segovia Donato 
Published: February 2007

AbstractAn active subpopulation of T lymphocytes characterized by their ability to form early rosettes with sheep erythrocytes (active E‐RBL) was studied in the blood of 50 patients with untreated systemic lupus erythematosus (SLE) and in 50 normal controls. The findings were related to the absolute number of circulating lymphocytes and total E‐receptor‐bearing lymphocytes (total E‐RBL). Lupus patients with active disease had markedly decreased absolute lymphocyte counts, but the decrease of both the total and the active E‐RBL surpassed what would be expected from the lymphopenia. Patients with inactive disease had moderately decreased absolute lymphocyte counts with a marked and disproportionate decrease in total E‐RBL and a moderate decrease in active E‐RBL, which seemed to reflect only the absolute lymphopenia. Patients with active disease had significantly lower active E‐RBL than those with inactive disease. The changes of these and other lymphocyte subpopulations in relation to disease activity in SLE may reflect the influence of factors leading to T‐cell depletion and immaturity. Circulating thymic products may be one of those factors.
Local and Systemic Reactions to Puncture Injuries by the Sea Urchin Spine and the Date Palm Thorn
Authors: Cracchiolo Andrea , Goldberg Leonard 
Published: February 2007

AbstractPuncture wounds were caused in 9 patients by sea urchin spines and 1 patient by a date palm thorn. All 10 patients developed local inflammatory reactions, which were moderate to severe in 7. Two patients exhibited severe systemic illnesses, and a marked synovitis was present in 2 others. Several of the patients presented as difficult diagnostic problems with signs and symptoms suggestive of an arthritic disease. Six required surgical removal of the foreign body.
The in Vivo Metabolism of the Third Component of Complement in Systemic Lupus Erythematosus
Authors: Petz Lawrence D. , Powers Runas , Fries James R. , Cooper Neil R. , Holman Halsted R. 
Published: February 2007

AbstractThe in vivo metabolism of purified C3, the third component of complement, labeled with 125‐iodine (125I‐C3) was studied in 17 normal volunteers and in 24 patients with systemic lupus erythematosus (SLE). The latter had varying degrees of disease severity and differing clinical and serologic manifestations. The study sought correlations of patterns of complement metabolism with clinical and laboratory parameters of disease activity. The data indicate that an increased fractional catabolic rate (FCR) of C3 is characteristic of active SLE because the FCR was increased in 14 of 16 patients with active disease but in only 1 of 8 patients with clinically inactive disease. This relationship is further emphasized by the fact that the FCR was increased in all patients with anti‐DNA antibodies and in all patients judged to have severe disease activity. All patients with normal values for FCR and C3 synthesis rate had clinically inactive disease and no patient judged to have active disease had normal values for both determinations. The synthesis rate of C3 was reduced in a total of 10 patients, but this did not correlate with disease activity, duration of disease, therapy, or certain other laboratory data. Causes of hyposynthesis have not been defined although two clinical settings in which it was found were late stage renal disease with inactive SLE, and severe disease activity with hypercatabolism of C3 and a profound reduction in serum C3 concentration. Hypersynthesis of C3, possibly as a compensatory mechanism, was found in 5 of the 7 patients with the highest FCR, one of whom had a normal level of C3 in spite of hypercatabolism. Such data indicate that various mechanisms determine serum C3 levels. This recognition may allow for more knowledgeable clinical interpretation of serum complement concentrations in SLE.
Discordant Distribution of IgM and IgG Antibodies to DNA and RNA in Monozygotic Twins with Systemic Lupus Erythematosus
Authors: Pillarisetty Rao J. , Block Sidney R. , Talal Norman 
Published: February 2007

AbstractSera from six sets of twins (five monozygotic and one dyzygotic) in whom one or both has systemic lupus erythematosus (SLE) were evaluated for antibodies to DNA and RNA. Sera from three monozygotic twin sets were further studied to determine the distribution of 19S and 7S antibodies to DNA and RNA.The presence of significant binding of polyriboadenylic acid and of native DNA correlated with the presence of clinical SLE in this study. Sucrose density gradient fractionation studies of the sera revealed that the clinically normal twins had some binding of Poly A and DNA limited to the 19S region, whereas the twins with SLE generally had significant levels of 19S and 7S antibodies to DNA and/or Poly A. On the other hand, concordance for presence or absence of antibodies to double‐stranded RNA was demonstrated within each twin set irrespective of concordance or discordance for clinical SLE. These results suggest that genetic factors may be important in determining which nucleic acids antigens become immunogenic, but genetic factors alone do not determine the immunoglobulin class distribution of antibodies to nucleic acids.
Depressed in Vitro B—Lymphocyte Differentiation in Systemic Lupus Erythematosus
Authors: Bobrove Arthur M. , Miller Patricia 
Published: February 2007

AbstractPeripheral blood lymphocytes from 20 patients with systemic lupus erythematosus (SLE) and 21 normal donors were incubated with pokeweed mitogen in order to assess in vitro terminal—differentiation of B lymphocytes into cells synthesizing intracytoplasmic immunoglobulin (Ig). Although the percentage (mean ± SEM) of B lymphocytes bearing surface Ig in the initial cell suspensions was not statistically different in SLE than in normal subjects (15 ± 2.2% versus 16 ± 1.9%, respectively), the frequency of cells containing intracytoplasmic Ig per 103 mononuclear cells was significantly lower in mitogen—stimulated cultures derived from the patients than from the normal controls (10 ± 2.3 versus 56 ± 13.0 for IgM, P < 0.01; 21 ± 3.6 versus 63 ± 10.4 for IgG, P < 0.01; 13 ± 3.1 versus 24 ± 3.8 for IgA, P < 0.05 respectively). Coculturing active SLE lymphocytes with cells from normal subjects resulted in a significant (P < 0.05) increase in the frequency of cells containing intracytoplasmic IgG when stimulated with pokeweed mitogen. Moreover, culturing SLE lymphocytes in cell—free media derived from activated normal lymphocytes also resulted in a significant increase in the frequency of IgG—containing cells. These results suggest that B—lymphocyte differentiation in vitro is depressed in SLE and may, at least partially, be reversed by products derived from normal lymphocytes.
On the Nature of Rheumatoid Rice Bodies
Authors: Berg Ed , Wainwright Randy , Barton Betty , Puchtler Holde , Mcdonald Thomas 
Published: February 2007

AbstractThe nature of rice bodies was studied, utilizing histochemistry, immunofluorescence, and scanning and transmission electron microscopy. Rice bodies were found to consist primarily of fibrous material, most of which was fibrin with small amounts of collagen. Channels containing a variety of viable cells permeated the rice bodies. Blood vessels occurred in a few rice bodies indicating a former connection with the synovial membrane. Nonvascularized rice bodies might represent a further degeneration of the vascular type. Rice bodies seem to be a nonspecific response to inflammation.
The Effect of Synovial Tissue on the Breakdown of Articular Cartilage in Organ Culture
Authors: Fell Honor B. , Jubb R. W. 
Published: February 2007

AbstractLiving (pig) cartilage in contact with synovium lost both proteoglycan and collagen and sometimes became reduced to a mass of fibroblast‐like chondrocytes without matrix; dead cartilage lost proteoglycan but less collagen. Similar changes appeared in living cartilage grown at a distance from the synovium but in the same dish; dead cartilage was unaffected. Conclusion: the synovium has a) a direct, presumably enzymatic action on cartilage matrix and b) an indirect effect mediated through the chondrocytes.
Zymosan—Induced Arthritis
Authors: Keystone E. C. , Schorlemmer H. U. , Pope C. , Allison A. C. 
Published: February 2007

AbstractZymosan particles, which are able to activate complement by the alternative pathway and to induce enzyme secretion from macrophages, were injected into knee joints of mice. After various time intervals, synovia were assessed histologically for various markers of inflammation. Within 7 days after intraarticular injection of zymosan, a chronic inflammatory arthritis with mononuclear cell infiltration, synovial hypertrophy and pannus formation was observed. Latex particles, which do not activate complement or macrophages, produced only mild, transient inflammatory reactions.
Use of a Hapten‐Carrier System in Experimental Immune Arthritis in the Rabbit
Authors: Ménard Henri A. , Demers Jean‐Claude 
Published: February 2007

AbstractAnimals immunized systemically with dinitro‐phenylated‐bovine serum albumin (DNP‐BSA) in complete Freund's adjuvant, show delayed hypersensitivity skin responses to DNP‐BSA and to BSA, but not to other DNP‐conjugates. These animals show high levels of antibodies to BSA and to DNP by radioimmunoassay. When injected with the carrier (BSA), the right knee joint develops a chronic arthritis. The left knee, when injected with the DNP hapten conjugated to another carrier (human serum albumin), shows an acute arthus response of the same magnitude as the right knee, but fails to develop a chronic synovitis. It appears that in this model, chronicity is not mediated primarily by a humoral mechanism to the initiator.
Interaction of D‐Penicillamine with Gold Salts
Authors: Davis P. , Barraclough D. 
Published: February 2007

AbstractSerum and urinary gold levels were monitored in 18 patients previously treated with gold salts for rheumatoid arthritis and the effects of D‐penicillamine studied. There was no statistically significant change in urinary gold levels on D‐penicillamine therapy although there were some individual variations. Serum gold levels fell during D‐penicillamine therapy but the rates of fall did not differ from those seen in patients not treated. In vitro studies on protein binding of gold salts suggest that a high affinity exists between gold salts and albumin with low levels of unbound gold even at concentrations far exceeding those seen in vivo. These preliminary results suggest that at therapeutic levels only small amounts of gold are available for chelation by penicillamine. It is concluded that penicillamine at low dosage is an unreliable chelator of gold salts in vivo and its use in the management of gold toxicity remains speculative.
Localization of C‐reactive protein in synovium of patients with rheumatoid arthritis
Authors: Gitlin Jonathan D. , Gitlin Joan I. , Gitlin David 
Published: February 2007

AbstractSynovial biopsies from 8 patients with rheumatoid arthritis, 2 patients with degenerative osteoarthritis, and 4 patients with nonarthritic disease were studied for localization of C‐reactive protein (CRP) using immuno‐fluorescence microscopy. The nuclei of many synoviocytes and histiocytes in rheumatoid synovial membrane were found to bind CRP. Cultures of rheumatoid synovium in 14C‐labeled amino acids produced radioactive IgG, IgM, IgA, and C3, but not CRP, indicating the synovial‐bound CRP was not of local origin. A few CRP‐binding nuclei were present in one osteoarthritic synovium, but none was found in the other and none in synovium from the 4 non‐arthritic patients. The nature of the nuclear CRP ligand in rheumatoid synovium was not determined.
Homozygous C2 deficiency with fulminant lupus erythematosus. severe nephritis via the alternative complement pathway
Authors: Gewurz Anita , Lint Thomas F. , Roberts Jimmy L. , Zeitz Howard , Gewurz Henry 
Published: February 2007

AbstractAbsence of C2 was identified in an 18‐year‐old white male with progressive and rapidly fatal lupus nephritis. Genetic studies of the patient and 8 family members linked this deficiency with the HLA haplotype A10/B18, for which the patient was homozygous. High titers (1:1600) of anti‐RNP antibodies, as well as antibodies to double‐stranded DNA, were present. Serum levels of properdin factor B were persistently decreased, whereas levels of C1q and C3 intermittently were low. Biopsies of skin and kidney showed typical SLE histopathology with deposition of immunoglobulins and early‐ and late‐acting C components; properdin was deposited in the kidney. Despite the inability of the patient's serum to mediate lysis through the classic C pathway, C‐dependent lysis through the alternative pathway was readily achieved using either unsensitized rabbit cells in gels or mixtures of guinea pig cells and zymosan as the indicator system. These studies thus reveal a new association of fatal lupus nephritis with homozygous C2 deficiency and show the usefulness of two new assays for measuring the hemolytic capacity of the alternative C pathway in human serum. The decreased levels of properdin factor B in serum, deposition of properdin and C3 in renal tissue, and capacity of C2‐deficient serum to mediate hemolytic C activity through the properdin pathway in vitro implicate the alternative C pathway in the severe tissue damage observed.
Effect of systemic lupus erythematosus antibodies against DNA on rna synthesis
Authors: Makarova Olga V. 
Published: February 2007

AbstractThe majority of tested systemic lupus erythematosus (SLE) sera inhibited RNA synthesis in vitro on the stage of RNA chain elongation. Two sera were also active in inhibiting the binding of the enzyme to the template. No correlation has been found between the sera activity in filter radioimmunoassay, their specificity to double‐ or single‐stranded DNA, and the degree of RNA synthesis inhibition.