bluuebunny/crossref_metadata_2025_split huggingface dataset:
G-Boxes, Bigfoot Genes, and Environmental Response: Characterization of Intragenomic Conserved Noncoding Sequences inArabidopsis
Authors: Freeling Michael , Rapaka Lakshmi , Lyons Eric , Pedersen Brent , Thomas Brian C. 
Published: May 2007

AbstractA tetraploidy left Arabidopsis thaliana with 6358 pairs of homoeologs that, when aligned, generated 14,944 intragenomic conserved noncoding sequences (CNSs). Our previous work assembled these phylogenetic footprints into a database. We show that known transcription factor (TF) binding motifs, including the G-box, are overrepresented in these CNSs. A total of 254 genes spanning long lengths of CNS-rich chromosomes (Bigfoot) dominate this database. Therefore, we made subdatabases: one containing Bigfoot genes and the other containing genes with three to five CNSs (Smallfoot). Bigfoot genes are generally TFs that respond to signals, with their modal CNS positioned 3.1 kb 5′ from the ATG. Smallfoot genes encode components of signal transduction machinery, the cytoskeleton, or involve transcription. We queried each subdatabase with each possible 7-nucleotide sequence. Among hundreds of hits, most were purified from CNSs, and almost all of those significantly enriched in CNSs had no experimental history. The 7-mers in CNSs are not 5′- to 3′-oriented in Bigfoot genes but are often oriented in Smallfoot genes. CNSs with one G-box tend to have two G-boxes. CNSs were shared with the homoeolog only and with no other gene, suggesting that binding site turnover impedes detection. Bigfoot genes may function in adaptation to environmental change.
Body mass index and oxidative DNA damage: A longitudinal study
Authors: Mizoue Tetsuya , Tokunaga Shoji , Kasai Hiroshi , Kawai Kazuaki , Sato Masao , Kubo Tatsuhiko 
Published: May 2007

Leanness has been shown to be related to an increased risk of some cancer forms, including lung cancer. However, biological evidence supporting a causal link between leanness and carcinogenesis is limited. The authors investigated longitudinally the association between body mass index (BMI) and levels of urinary 8‐hydroxydeoxyguanosine (8‐OHdG), a marker of oxidative DNA damage, using data from 174 healthy employees who participated in a lifestyle intervention study. 8‐OHdG levels were measured using automated high‐performance liquid chromatography and adjusted for urinary creatinine levels. Analysis of repeated measurements using a random effects model detected a statistically significant inverse association between BMI and 8‐OHdG levels (P = 0.003); one unit decrease in BMI was associated with a 2.7% (95% confidence interval 0.9–4.4) increase in 8‐OHdG levels. The association was pronounced among men consuming less than 20 cigarettes per day (8.8% increase per unit decrease in BMI) and among non‐smoking men (3.7% increase). The results based on a longitudinal observation suggest that weight loss is associated with increased oxidative DNA damage, a state presumably related to an increased risk of cancer. (Cancer Sci 2007; 98: 1254–1258)
NOUVELLES RECHERCHES ELECTRO‐ENCEPHALOGRAPHIQUES DANS L'ASTHME BRONCHIQUE
Authors: PANZANI R. 
Published: May 2007

SUMMARYTwo hundred and forty EEG observations bring out this conclusion: in asthma 65 per cent of the observed cases show a disturbed cortical electro genesis leading to the hypothesis of a hyperexcitability or less frequently a hypoexcitability and possibly an actual depression of meso‐diencephalic reticular substance.The first hypothesis was that this major or minor dysfunction of the reticular substance is one of the causes of asthma and not the consequence of an oedema of the brain caused by repeated attacks. Out recent EEG studies would incline in some cases in favour of the latter hypothesis.We have observed in 17 out of 80 regularly followed cases, that minor anomalies in adults and even major anomalies in children have disappeared. Asthma has been practically always allergic in children so we think that the electrical anomalies which have spontaneously disappeared were due to an allergic oedema of the brain.
STUDIES OF THE WHEAL REACTION OF NORMALS TO INTRADERMAL INJECTION OF SERUM FROM ALLERGIC SUBJECTS, WITH HOMOLOGOUS ALLERGEN ADDED
Authors: BERGQUIST G. 
Published: May 2007

SUMMARYWhen homologous allergen was added to serum from allergic patients and the mixture injected intradermally into normal subjects, wheal reactions resulted. The intensity of this edematigenous effect of certain sera showed individual variability. With the method here employed, the effect was thermostable.
EVALUATION CHIFFREE DE L'ETAT CLINIQUE DE MALADES ATTEINTS DE POLLINOSE AUX GRAMINEES
Authors: Planes M. 
Published: May 2007

MM. C. Boutin, J. P. Orlando, H. Raztouk et J. Charpin utilisent une méthode mathématique pennettant d'apprécier le plus objectivement possible l'etat clinique des malades atteints de pollinose aux graminées. Le patient est inviteéà tenir un journal, retranscrit sur bordereau de perforation utilisable en ordinateur et traité par un programme de régression multiple pas à pas. On aboutit ainsi à un « score », à une « note globale » reflétant l‘état du malade pendant une saison et permettant des études comparatives. On peut aussi chercher des corrélations entre la courbe quotidienne des symptômes, les comptes palynologiques et les données météorologiques. Le calcul statistique permet d'apprécier le dégre de cette méthode.Un groupe de 117 malades a été ainsi etudié en 1969, 1970 et 1971. Le traitement statistique a montré que l'efficacité des désensibilisations depend du type d'extrait utilisé, les extraits aqueux donnant de meilleurs résultats que les extraits‐retard. La presence d'asthme, et sa gravité, sont des éléments de pronostic défavorables. II existe une correlation entre les symptômes journaliers et la courbe de pollinisation. Mais la corrélation entre le score et les données météorologiques n'est pas stricte. On observe seulement une élévation du score aux périodes ensoleillées où la température maxima est la plus élevée, la pluie absente, le vent modéré, la pression atmosphérique moyenne relativement stable.Le caractère subjectif des journaux de malades donne lieu à plusieurs interventions. Chez l'asthmatique, M. Gervais suggère l'emploi régulier de la spirométrie de pointe.
BECLOMETHASONE DIPROPIONATE AEROSOL IN REVERSIBLE OBSTRUCTIVE AIRWAYS DISEASE
Authors: LANGAKER KJELL ERIK 
Published: May 2007

Seven patients with reversible obstructive airways disease, who were unsatisfactorily relieved by conventional broncho‐dilating drugs, were admitted to a 1‐year‐long therapeutic trial with beclomethasone dipropionate aerosol, 400 μg a day.After 3 weeks of treatment the mean values of VC, FEV, and PEFR were increased by about 100 per cent of the pre‐treatment values, and the consumption of self‐administered bronchodilators was markedly diminished.Throughout the trial the occupational disability and need for hospitalisation were negligible compared with the previous year. Development of tolerance to the drug was not observed during the trial.The adrenocortical function remained unaffected, as judged by the plasma cortisol levels and adrenocortical stimulation tests. Continuously low normal levels of excreted urinary 17‐ketogenic‐steroids might indicate a very slight adrenal suppression.
ASSOCIATION OF PRECIPITINS AND COELIAC DISEASE
Authors: KAVAI M. , CSORBA S. , SZABOLCS M. , JEZERNICZKY J. , FÉSÜS L. , SZABÓ B. 
Published: May 2007

SUMMARYFour fractions, fraction A, B, C, and D, were isolated from the aqueous extract of wheat flour on a Sephadex G‐75 column. It was found that the repeated gel‐filtrated fraction A reacts as an antigen with the sera of coeliac patients. On the basis of its molecular weight and amino acid composition, it is gliadin.Each of the 28 coeliac patients had heat‐stable precipitating antibody and higher than normal IgE levels were found in the sera of only three individuals. Specific antigliadin IgE in low quantity was found in only one case.The sera reacting with antigens in high titres were absorbed by anti‐IgG and anti‐IgA whereupon the antibody level considerably decreased. The main circulating antibody of the patients was IgG.
Comparison of the Effect of the β1‐Blocking Drugs Atenolol and Metoprolol on Bronchial Asthma
Authors: Carlson Christer , Järvinen Klaus A. J. 
Published: May 2007

Twenty patients with severe or medium severe asthma were given atenolol (Atenor® (CI – Pharma) and/or metoprolol (Soleken® Hässle) for tachycardia, hyperkinetic tremor, arterial hypertension or symptoms of angina pectoris. These cardioselective β‐blocking drugs caused only a very slight decrease in PF values. There was no difference between atenolol and metoprolol as regards the PF values A 24 hourly dose of 100 mg atenolol caused a distinct fall in diastolic pressure as compared with the same amount of metoprolol. Both these two β1‐blockers moderated the tachvcardia which occurs in asthma; atenolol in this dose had a slightly stronger action.The subjective condition of five patients with severe or medium severe asthma was considerably relieved by atenolol and/or metoprolol. The relief manifested as a lessening of dyspnoea and improvement of the general status. No essential change was observed in the PF values despite the subjective effects. The most noteworthy change was the amelioration of tachycardia which had continued longer than expected in these patients. The heart rate dropped from 140–120/min to 90–70/min and dyspnoea was relieved at the same time.
Use of RAST Technique in Wasp Sting Hypersensitivity
Authors: Müller U. , Roth A. , Yman L. , Patrizzi R. 
Published: May 2007

Clinical hypersensitivity to wasp stings was found to be fairly well correlated with the presence of serum IgE‐antibodies against yellow jacket venom as detected by the RAST technique. Such antibodies were never found in a control group of non‐allergic blood donors, but they were detected in a surprisingly large proportion of patients with bee sting allergy without known allergic reactions to wasps. Studies using RAST inhibition technique failed to prove cross‐reactions between bee and wasp venoms.Considerably better results were obtained when venom antigens instead of whole body antigens were used in the RAST, RAST inhibition studies suggested that IgE‐antibodies detected with RAST using whole body antigen are directed against bee venom constituents in the whole body extract.
Binding of Antigen to Surface to on Isolated Rat Mast Cells
Authors: Stahl Skov P. , Norn S. , Wiik A. , Permin H. 
Published: May 2007

This study concerns observations on isolated rat mast cells sensitized to specific antigen. Specific binding of antigen to the surface immunoglobulins of these cells could be demonstrated by immunofluorescence technique as well as by radiolabelled antigen binding and the degree of binding parallelled the allergic reaction as judged by histamine releaser Exposure of mast cells to antigen at both Low and high antigen concentrations did not change the distribution of the surface immunoglobulins. Furthermore, neither capping nor shedding could be induced, even by excessive antigen stimulation, The amount of antigen molecules bound to surface Ig was linearly correlated lo the allergic histamine release. When mast cells were sensitized to two antigens of different molecular weight, the cells showed the highest sensitivity to the antigen having the highest molecular weight.These results indicate that both the amount of antigen and the molecular weight of the antigen bound to surface Ig are important factors in the allergic histamine release from mast cells, whereas surface Ig redistribution is nor.
Allergy to Pollen from Different Deciduous Trees in Sweden
Authors: Eriksson Nils E. 
Published: May 2007

92 adult patients with springtime allergy were investigated with skin tests, provocation tests and the RAST. Allergen extracts from 12 different deciduous trees were used.Positive reactions, often of high intensity, were most often found with birch, alder, bog‐myrtle, beech and hazel allergens whereas oak, aspen, linden, elm, sallow, maple and poplar allergens more often gave negative or only weak positive test results. Cross sensitizations were found between botanically related as well as between less related species of the trees. Almost all patients with a clinically relevant tree pollen allergy had positive provocation tests with birch pollen. No patient had positive reactions to all the 12 allergens included in the study. 68% had positive provocation tests with the combination birch, alder and hazel.It is concluded that for diagnostic screening purposes it is sufficient to use birch pollen allergen. If the majority of relevant tree pollens are to be included in a diagnostic or therapeutic programme in Western Sweden it should contain birch, alder, hazel, beech and bog‐myrtle allergens.
Prevention of Experimental Anaphylactic Ulcer by Antigen Prefeeding
Authors: Andre F. , Andre C. , Fournier S. 
Published: May 2007

Studies were performed in animals to establish whether antigen preleeding could prevent homocytotropic antibody synthesis and the induction of gastric ulcer resulting from mucosal anaphylaxis.A single digestive exposure to 1 or 10 mg ovalbumin induced a state of specific immunologic tolerance. Inhibition of the Formation of specific regains was shown by the study of mast cell degranulation.Tolerant animals presented a reduced incidence of gastric ulcer alter subsequent mucosal challenge.These results are important for the development of methods of prevention and treatment of allergic diseases.
Penicillin Hypersensitivity
Authors: de Haan P. , Boorsma D. M. , Kalsbeek G. L. 
Published: May 2007

An enzyme‐linked immunosorbent assay for the detection of anti‐penicillin antibodies of the several Ig classes is described. The results of the ELISA in 350 sera of patients suspected of penicillin hypersensitivity are compared with those of the haemagglutination test.In 105 sera penicillin‐specific IgM and/or IgG was demonstrated with the ELISA, the HA test being positive in 49 of these 105 sera. However, in another 14 sera IgM anti‐penicillin antibodies could be shown only with the HA.In 10 sera penicillin‐specific IgE was demonstrated with the ELISA, only four of these being also positive with the RAST. IgE was always found in combination with IgG and/or IgM.The positive correlation of the ELISA and the RAST with the intracutaneous test on penicilloylpolylysine was 26.9% and 15.4% respectively.The ELISA is a simple and reproducible method for the detection of anti‐penicillin antibodies, being more sensitive than the HA and the RAST.
The Protective Effect of a New Agent, Ketotifen Syrup, in the Treatment of Childhood Asthma
Authors: ØSterballe Ole , Addi Lykkegaard Nielsen Elisabeth 
Published: May 2007

Twenty‐two children with perennial extrinsic allergic asthma participated in a double‐blinded, controlled, cross‐over study of Ketotifen syrup during a 2 × 6‐week period. The placebo was identical with the active drug in taste, colour, and consistency. The dosage level was, on weight basis, a little higher than the standard dosage for adults.The double‐blinded investigation was supplemented with a 12‐week open study of the tolerance for Ketotifen. This study also involved 22 patients, 12 new ones, and 10 from the double‐blinded study.The study showed Ketotifen to have no effect on changes in lung function (peak How), on reduction in frequency of asthmatic attacks, or on consumption of additional medicine.The tolerance for Ketotifen was good, but physicians are advised to watch for increases in weight and decreases in blood pressure during tests on children which involve higher dosage levels.
Analysis of Antigens in a Commercial House‐Dust Extract by Means of Quantitative Immunoelectrophoresis
Authors: Carlsen S. D. , Weeke B. , Løwenstein H. 
Published: May 2007

Crossed immunoelectrophoresis (CIE) of a commercial house‐dust extract revealed 43 immunoprecipitates.Rocket immunoelectrophoresis with 106 different allergen extracts from animal hair and dander, feathers, fungi, pollen, foodstuffs, textiles and house‐dust mites showed the presence of precipitating antibodies in the rabbit anti‐house‐dust serum against antigens from 34 of the extracts.Crossed immunoelectrophoresis with the addition of antigen, and crossed line immunoelectrophoresis (CLIE) with these 34 allergen extracts, as well as crossed immunoelectrophoresis with intermediate gel containing rabbit antibodies against nine human serum proteins, allowed the identification of 14 antigens in the house‐dust extract. These antigens were shown to originate from house‐dust mites, hair and dander from various animals, fungi, feathers, human hair and dander, and human serum proteins.
Tarsonemid Mites in Danish House Dust
Authors: Korsgaard Jens , Hallas Thorkil E. 
Published: May 2007

From a study of 98 typical Danish city apartments it was found that pyroglyphid and tarsonemid mites dominated the housedust acarofauna. Both groups of miles show a remarkable similarity in their occurrence and dependence on. the microclimate. Room air humidities above 7 g water/kg dry air involved the highest densities. Below this Unit only background contamination appeared. At least 14 distinct forms of tarsonemid mites were found, with Tarsonemiu floricolus Can. & Fanz., 1876 as the dominating species.As tarsonemid mites were found regularly rather than occasionally, it is suggested that these mites may be responsible for at least a part of the cases of housedust allergy thaE cannot be ascribed to the content of Dermatophagoides mites in the housedust.
Epicutaneous Testing
Authors: Hannuksela Matti 
Published: May 2007

The patch test and chamber test are the ordinary methods for detecting delayed type contact allergies. The method, test substances and criteria of positive reactions are already well standardized. Open testing is also used for problematic chemicals such as strong topical medicaments, emulsifiers and propylene glycol.The many problems of standardization are still unresolved in photocutaneous testing. Some chemicals are applied to the skin without occlusion, though the chamber test or patch test methods are used with most of them. The composition of the vehicle and occlusion time vary greatly from one photosensitizing substance to another. A xenon are is recommended for screening any photosensitizers but fluorescent black light tubes are sufficient for routine clinical use.Type I contact allergies are far more common than was previously believed, especially among people allergic to birch pollen. This type of reaction and other forms of contact urticaria can he examined using the scratch chamber or scarification chamber techniques as well as with an open test on diseased or scratched (scarified) skin.
Binding of Antigen to Surface Ig during in vitro Hyposensitization of Isolated Rat Mast Cells
Authors: Skov P. Stahl , Norn S. , Wiik A. 
Published: May 2007

The mechanism of in vitro hyposensitization was examined in isolated rat mast cells. Surface distribution of immunoglobulin was examined by immunofluorescence technique. Hyposensitization could be ascribed neither to changes in the distribution of surface immunoglobulins nor to capping or shedding phenomena. The distribution of cell‐bound antigen and the number of antigen‐positive cells (patch‐bearing cells) was not changed by hyposensitization. The binding of antigen to surface Ig was quantitated by I125‐labelled antigen. In rats sensitized to two different antigens, hyposensitization of the mast cells with one of these inhibited the subsequent histamine release induced by either of the two antigens, whereas no changes were observed in the antigen binding capacity.The present investigation indicates that the mechanism of in vitro hyposensitization is the result of events secondary to the initial binding of antigen to the mast cell surface immunoglobulin.
Occupational Allergy in Nurses to a Bulk Laxative
Authors: Machado Lavinia , Zetterström Olle , Fagerberg Erik 
Published: May 2007

Finely ground psyllium (Plantago ovata) seeds, so‐called ispaghula powder is used in bulk laxatives. During the dispensing of these medicines some dust is generated. Allergic symptoms from exposure to this dust was reported by two nurses, one of whom was working in a department where exposure to the powder could be considered high. Nineteen other subjects working in the same department were investigated with regard to allergy to ispaghula powder.An extract from ispaghula powder was made and used in RAST and skin tests. Exposure tests with ispaghula powder mixed with crushed rusks were made in symptomatic subjects. Eight out of the 20 investigated subjects from this department reported symptoms which could possibly have been elicited by ispaghula powder. Evidence of sensitization was found in five of them.The use of less dusty (granulated) forms of ispaghula laxatives might diminish the risk of sensitization.
Immunotherapy in Bee Sting Hypersensitivity
Authors: Müller U. , Thurnheer U. , Patrizzi R. , Spies J. , Hoigné R. 
Published: May 2007

Fifty‐six patients with serologically confirmed bee sting hypersensitivity were treated for 1–3 years with either bee venom (BV) (31 patients) or wholebody extract (WBE) (25 patients). Twelve patients in each group were re‐exposed to a bee sting. Twenty‐five per cent of re‐exposed patients on BV‐therapy showed a diminished reaction and 75% no reaction. Thirty‐three per cent of re‐exposed patients on WBE‐therapy developed an unchanged or worse reaction, 42% a diminished reaction and 25% no reaction at all.An initial rise in BV‐specific IgE was observed in BV‐treated patients, whereas IgE levels after 1 year of treatment lay significantly below pretreatment values in both treatment groups. BV‐specific IgG increased markedly in patients on BV‐therapy and decreased slightly in those on WBE‐therapy. The initial IgE increase induced by BV‐therapy was insignificant in patients with high pretreatment levels of specific IgG‐antibodies. Similarly, re‐exposed patients with high BV‐specific IgG showed no IgE increase, whereas a substantial rise in specific IgE was observed in those with low IgG. On the basis of clinical and serological results immunotherapy with BV is considered to be clearly superior to immunotherapy with WBE
Pseudomonas Aeruginosa Allergy in Cystic Fibrosis
Authors: Skov P. Stahl , Norn S. , Schiøtz P. O. , Permin H. , Høiby N. 
Published: May 2007

Basophil histamine release by P. aeruginosa standard antigen was examined in cystic fibrosis patients chronically infected with mucoid P. aeruginosa (CF + P) and with pronounced antibody response against these bacteria, and in patients without P. aeruginosa infection (CF ‐ P). All the patients showed eosinophil counts and total IgE, which did not differ significantly from that of normal persons. In the absence of patient's sera, histamine release was only found in two patients in the CF + P group, indicating that type I allergy to P. aeruginosa is not predominating in cystic fibrosis.In the presence of patients' sera significantly more of the CF + P patients responded to P. aeruginosa with histamine release compared with the CF ‐ P patients. The response was lost by complement inactivation and regained by reconstitution of the complement activity. The involvement of a type III‐mediated complement‐dependent histamine release is therefore suggested in the pathogenesis of lung damage in cystic fibrosis.