bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telomere-to-telomere genome assembly of the clubroot pathogenPlasmodiophora brassicae
Authors: Javed Muhammad Asim , Mukhopadhyay Soham , Normandeau Eric , Brochu Anne-Sophie , Pérez-López Edel 
Published: April 2024

ABSTRACTPlasmodiophora brassicae (Woronin, 1877), a biotrophic, obligate parasite, is the causal agent of clubroot disease in brassicas. The clubroot pathogen has been reported in more than 80 countries worldwide, causing economic losses of hundreds of millions every year. Despite its widespread impact, very little is known about the molecular strategies it employs to induce the characteristic clubs in the roots of susceptible hosts during infection, nor about the mechanisms it uses to overcome genetic resistance. Here, we provide the first telomere-to-telomere complete genome of Plasmodiophora brassicae. We generated ∼ 27 Gb of Illumina, Oxford Nanopore, and PacBio HiFi data from resting spores of strain Pb3A and produced a 25.3 Mb assembly comprising 20 chromosomes, with an N50 of 1.37 Mb. The BUSCO score, the highest reported for any member of the group Rhizaria (Eukaryota: 88.2%), highlights the limitations within the Eukaryota database for members of this lineage. Using available transcriptomic data and protein evidence, we annotated the Pb3A genome, identifying 10,521 protein-coding gene models. This high-quality, complete genome of Plasmodiophora brassicae will serve as a crucial resource for the plant pathology community to advance the much-needed understanding of the evolution of the clubroot pathogen.SIGNIFICANCEPlasmodiophora brassicae (Woronin, 1877) is a devastating plant pathogen, member of the Rhizaria group, and is putting at risk the oilseed rape and cruciferous vegetable industry worldwide. Here, we present the first telomere-to-telomere genome of P. brassicae, and the first complete genome for a member of the Rhizaria taxon. We also provide a high-quality genome annotation featuring an enhanced gene model relative to previous studies. This complete genome will be of great interest to researchers focused on the ecology and evolution of the clubroot pathogen, as well as those studying other members of the Rhizaria group.
Exploring critical success factors for digital transformation in construction industry–based on TOE framework
Authors: Zhong Yi , Chen Zhiqian , Ye Jinglei , Zhang Na 
Published: April 2024

PurposeThis study aims to investigate the critical success factors of digital transformation in the construction industry and identify whether the respondents' profiles influence their perceptions of critical success factors for digital transformation.Design/methodology/approachTo achieve the objectives, a literature review was first conducted based on technology-organization-environment (TOE) framework. Then a questionnaire survey was carried out. A total of 86 people were surveyed in this study, mainly from the construction industry. At the level of data processing, SPSS was used for analysis. Among the main tests used were the Shapiro–Wilk test, reliability analysis, mean rank analysis, Kruskal–Wallis test and Mann–Whitney U test.FindingsThe study identified 15 critical success factors of digital transformation and found the three most important factors of digital transformation. Furthermore, respondents with different years of experience, enterprises with different sizes and different years made no difference in the perception of factors. Respondents' different occupations and types of enterprises created a bias in the perception of factors for digital transformation.Research limitations/implicationsFirstly, the small sample size of the questionnaire limits the reference value of data analysis for certain groups. In addition, this study focuses broadly on construction enterprises without specifically examining different types of enterprises, thus lacking depth in its findings.Practical implicationsThis study establishes a connection between TOE theory and the construction industry through an extensive literature review, identifying relevant factors and providing a reference for future research.Originality/valueThe study's results would enrich the research on digital transformation in the construction industry and provide a reference for the digital transformation of construction enterprises.
Strengthening resilience in supply chains: the role of multi-layer flexibility, supply chain risks and environmental dynamism
Authors: Singh Rohit Kumar 
Published: April 2024

PurposeThis study examines the relationship between multi-layer supply chain flexibility (MSCF) and Supply chain resilience (SCR). Further, it looks at the moderating effect of environmental dynamism (ED) and supply chain risks (SCRI) on the relationship between MSCF and SCR.Design/methodology/approachExecutives from the pharmaceutical, agri-food, electronics, automobile and textile industries were invited to complete a self-administered questionnaire. We received feedback from a total of 302 participants. Prior to conducting the primary analysis, we addressed the potential for nonresponse bias and verified the assumptions of homoscedasticity and normal distribution of the data. The reliability and validity of the constructs were established through confirmatory factor analysis. Structural equation modelling is employed for the purpose of conducting hypothesis testing.FindingsThe results demonstrate a notable influence of MSCF on SCR, particularly in settings characterized by high levels of ED and SCRI. The study highlights the importance of flexibility in multiple aspects of the supply chain to build resilience against a range of disruptions and uncertainties.Originality/valueThe study presents the fundamental role of Multi-Layer Flexibility in building up SCR. The results of this study reinforce the existing literature and offers empirical evidence for how ED, SCRI moderates the influence between MSCF to SCR. These results offer valuable information to both supply chain specialists and researchers for building comprehensive strategy to bring resilience in supply chains.
Duality in barriers and enablers of augmented reality adoption in education: a systematic review of reviews
Authors: Albishri Baraa , Blackmore Karen L. 
Published: April 2024

Purpose
The study aims to identify the key advantages/enablers and disadvantages/barriers of augmented reality (AR) implementation in education through existing reviews. It also examines whether these factors differ across educational domains.

Design/methodology/approach
This study conducted a systematic review of reviews to synthesize evidence on the barriers and enablers influencing AR adoption in education. Searches were performed across five databases, with 27 reviews meeting the inclusion criteria. Data extraction and quality assessment were completed. Content analysis was conducted using the AR adoption factor model and consolidated framework for implementation research.

Findings
The findings reveal several enablers such as pedagogical benefits, skill development and engagement. Equally, multiple barriers were identified, including high costs, technical issues, curriculum design challenges and negative attitudes. Interestingly, duality emerged, whereby some factors served as both barriers and enablers depending on the educational context.

Originality/value
This review contributes a novel synthesis of the complex individual, organizational and technological factors influencing AR adoption in education across diverse domains. The identification of duality factors provides nuanced understanding of the multifaceted dynamics shaping AR integration over time. The findings can assist educators in tailoring context-sensitive AR implementation strategies to maximize benefits and minimize drawbacks. Further research should explore duality factors and their interrelationships in AR adoption.
A comparative study of Indian and Chinese textile and clothing exports in post-MFA environment
Authors: Meena Anita 
Published: April 2024

Purpose
This paper aims to examine and compare the export performance and competitiveness of Indian and Chinese textile and clothing industries post-multifibre arrangement (MFA) era.

Design/methodology/approach
Balassa’s revealed comparative advantage Index is used to assess the competitiveness of Indian and Chinese textile and clothing exports.

Findings
The results indicate that China’s textiles and garments sector holds a greater proportion of the global market compared with India. India has a robust comparative advantage in silk, carpets, and cotton post-MFA. Vegetable textile fibers, paper yarn, and woven fabrics of paper yarn are also competitive. China had a strong comparative advantage in silk and fabrics; special woven fabrics, tufted textile fabrics, lace, tapestries, trimmings, and embroidery in 2005. China also recorded comparative advantage in silk, man-made filaments: strip and the like of man-made textile materials, fabrics; special woven fabrics, tufted textile fabrics, lace, tapestries, trimmings, and embroidery and fabrics; knitted or crocheted in 2021.

Research limitations/implications
This study’s results and recommendations could assist the Indian and Chinese Governments develop policies to upgrade their garment industries.

Originality/value
Though vast literature reviews are available for textile and apparel export performance in India and China separately, there are few studies on comparisons. This study is a significant attempt to evaluate India and China’s competitiveness in the global market.
Potential use of yeast protein in terms of biorefinery, functionality, and sustainability in food industry
Authors: Timira Vaileth , Chen Xing , Zhou Peng , Wu Junjun , Wang Tao 
Published: April 2024

AbstractA growing demand for sustainable, alternative protein sources that are nutrient‐dense, such as microorganisms, and insects, has gradually evolved. When paired with effective processing techniques, yeast cells contain substantial substances that could supply the population's needs for food, medicine, and fuel. This review article explores the potential of yeast proteins as a sustainable and viable alternative to animal and plant‐based protein sources. It highlights the various yeast protein extraction methods including both mechanical and non‐mechanical methods. The application of nanoparticles is one example of the fast‐evolving technology used to damage microbial cells. SiO2 or Al2O3 nanoparticles break yeast cell walls and disrupt membranes, releasing intracellular bioactive compounds. Succinylation of yeast protein during extraction can increase yeast protein extraction rate, lower RNA concentration, raise yeast protein solubility, increase amino acid content, and improve yeast protein emulsification and foaming capabilities. Combining physical and enzymatic extraction methods generates the most representative pool of mannose proteins from yeast cell walls. Ethanol or isoelectric precipitation purifies mannose proteins. Mannoproteins can be used as foamy replacement for animal‐derived components like egg whites due to their emulsification, stability, and foaming capabilities. Yeast bioactive peptide was separated by ultrafiltration after enzymatic hydrolysis of yeast protein and has shown hypoglycemic, hypotensive, and oxidative action in vitro studies. Additionally, the review delves into the physicochemical properties and stability of yeast‐derived peptides as well as their applications in the food industry. The article infers that yeast proteins are among the promising sources of sustainable protein, with a wide range of potential applications in the food industry.
Radical Urban Classrooms: Civic Pedagogies and Spaces of Learning on the Margins of Institutions
Authors: Antaki Nicola , Belfield Andrew , Moore Thomas 
Published: April 2024

AbstractThis paper investigates the relationship between “civic pedagogy” and institutions, through three case studies: the Live Works Castlegate Co‐Production at the University of Sheffield; a Collective Design Pedagogy at Muktangan School, India; and the School for Civic Action at the Tate Exchange. The projects aim to build citizens’ agency with transformative urban potential by engaging civic pedagogy using different scales, perspectives, and resources: as engagement with schools, higher education institutions, and art practices. Using Pelin Tan's definition of urgent pedagogies as a framework, this paper investigates the alternative alliances, trans‐territorial solidarities, and transversal methodologies of each project, comparing and contrasting methods and techniques. We discuss the potential for a shared decentralisation of learning associated with institutions, by engaging with the urban environment and communities, and propose a series of codependent practices for the initiation and sustenance of civic pedagogy for geographers interested in urban learning and education.
Allergic reaction of poly‐ether‐ether‐ketone versus titanium implants: A posttest‐only control group design experimental study using a rabbit model
Authors: Hassan Tamer Hamed , Ayappali Kalluvalappil Nabeel 
Published: April 2024

AbstractPurposeThe aim of this study was to determine clinically and genetically the allergic effects of titanium and poly‐ether‐ether‐ketone (PEEK) implants following loading in rabbit tibias.Materials and methodsThis study included 18 white New Zealand male rabbits (n = 18) divided evenly into three groups: control, titanium (Ti), and PEEK (P). Clinically, the allergenic effect of titanium and PEEK was investigated by detecting the effect on lymph nodes. Furthermore, RT‐PCR and ELISA were used to detect the expression of certain genes IL‐6, TNF‐α, OPG, RANKL, and RUNX‐2 through both types of implants.ResultsOur findings demonstrated that titanium implants induced enlarged lymph nodes, which PEEK did not. Overall, RT‐PCR and ELISA techniques revealed that Ti implants had higher expression of the inflammatory genes IL‐6 and TNF‐α. Ti had the highest expression in OPG findings, while PEEK had the lowest. RANKL expression was highest in the control group and lowest in the PEEK group. RUNX‐2 is the highest for the control group and the lowest for the titanium group.ConclusionAlthough titanium implants elicited greater allergy responses than PEEK implants, titanium has the highest expression of bone formation genes and the lowest expression of bone resorption genes, making it preferable to PEEK.
Stigmasterol alleviates neuropathic pain by reducing Schwann cell‐macrophage cascade in DRG by modulating IL‐34/CSF1R
Authors: Si Waimei , Chen Zhenni , Bei Jing , Chang Shiquan , Zheng Yachun , Gao Li , Zhao Guoping , Li Xin , Zhang Di 
Published: April 2024

AbstractAimsThis study aimed to investigate the potential therapeutic applications of stigmasterol for treating neuropathic pain.MethodsRelated mechanisms were investigated by DRG single‐cell sequencing analysis and the use of specific inhibitors in cellular experiments. In animal experiments, 32 male Sprague–Dawley rats were randomly divided into the sham operation group, CCI group, ibuprofen group, and stigmasterol group. We performed behavioral tests, ELISA, H&E staining and immunohistochemistry, and western blotting.ResultsCell communication analysis by single‐cell sequencing reveals that after peripheral nerve injury, Schwann cells secrete IL‐34 to act on CSF1R in macrophages. After peripheral nerve injury, the mRNA expression levels of CSF1R pathway and NLRP3 inflammasome in macrophages were increased in DRG. In vitro studies demonstrated that stigmasterol can reduce the secretion of IL‐34 in LPS‐induced RSC96 Schwann cells; stigmasterol treatment of LPS‐induced Schwann cell‐conditioned medium (L‐S‐CM) does not induce the proliferation and migration of RAW264.7 macrophages; L‐S‐CM reduces CSF1R signaling pathway (CSF1R, P38MAPK, and NFκB) activation, NLRP3 inflammasome activation, and ROS production. In vivo experiments have verified that stigmasterol can reduce thermal and cold hyperalgesia in rat chronic compressive nerve injury (CCI) model; stigmasterol can reduce IL‐1β, IL‐6, TNF‐α, CCL2, SP, and PGE2 in serum of CCI rats; immunohistochemistry and western blot confirmed that stigmasterol can reduce the levels of IL‐34/CSF1R signaling pathway and NLRP3 inflammasome in DRG of CCI rats.ConclusionStigmasterol alleviates neuropathic pain by reducing Schwann cell‐macrophage cascade in DRG by modulating IL‐34/CSF1R axis.
Molecular mechanism analyses of post‐traumatic epilepsy and hereditary epilepsy based on 10× single‐cell transcriptome sequencing technology
Authors: Wen Fang , Tan Zhigang , Huang Dezhi , Xiang Jun 
Published: April 2024

AbstractBackgroundSingle‐cell RNA sequencing analysis has been usually conducted on post‐traumatic epilepsy (PET) and hereditary epilepsy (HE) patients; however, the transcriptome of patients with traumatic temporal lobe epilepsy has rarely been studied.Materials and MethodsHippocampus tissues isolated from one patient with PTE and one patient with HE were used in the present study. Single cell isolates were prepared and captured using a 10× Genomics Chromium Single‐Cell 3′ kit (V3) according to the manufacturer's instructions. The libraries were sequenced on an Illumina NovaSeq 6000 sequencing system. Raw data were processed, and the cells were filtered and classified using the Seurat R package. Uniform Manifold Approximation and Projection was used for visualization. Differentially expressed genes (DEGs) were identified based on a p‐value ≤0.01 and log fold change (FC) ≥0.25. Gene Ontology (GO, http://geneontology.org/) and KEGG (Kyoto Encyclopedia of Genes and Genomes, www.genome.jp/kegg) analyses were performed on the DEGs for enrichment analysis.ResultsThe reads obtained from the 10× genomic platform for PTE and HE were 39.56 M and 30.08 M, respectively. The Q30 score of the RNA reads was >91.6%. After filtering, 7479 PTE cells and 9357 HE cells remained for further study. More than 96.4% of the reads were mapped to GRCh38/GRCm38. The cells were differentially distributed in two groups, with higher numbers of oligodendrocytes (6522 vs. 2532) and astrocytes (133 vs. 52), and lower numbers of microglial cells (2242 vs. 3811), and neurons (3 vs. 203) present in the HE group than in the PTE group. The DEGs in four cell clusters were identified, with 25 being in oligodendrocytes (13 upregulated and 12 downregulated), 87 in microglia cells (42 upregulated and 45 downregulated), 222 in astrocytes (115 upregulated and 107 downregulated), and 393 in neurons (305 upregulated and 88 downregulated). The genes MTND1P23 (downregulated), XIST (downregulated), and RPS4Y1 (upregulated) were commonly expressed in all four cell clusters. The DEGs in microglial cells and astrocytes were enriched in the IL‐17 signaling pathway.ConclusionOur study explored differences in cells found in a patient with PE compared to a patient with HE, and the transcriptome in the different cells was analyzed for the first time. Studying inflammatory and immune functions might be the best approach for investigating traumatic temporal lobe epilepsy in neurons.
Okkludierende kutane Vaskulopathien als Ursachen chronischer Unterschenkelulzerationen
Authors: Ronicke Moritz , Berking Carola , Erfurt‐Berge Cornelia 
Published: April 2024

ZusammenfassungUnter den Begriff der okkludierenden Vaskulopathien fällt eine Reihe unterschiedlicher Krankheitsbilder. Diese manifestieren sich an der Haut häufig mit Ulzerationen. Gerade bei Ulzerationen der Unterschenkel sollten okkludierende Vaskulopathien in die differenzialdiagnostischen Überlegungen einbezogen werden. Der Begriff „okkludierende Vaskulopathien“ umfasst pathophysiologisch verwandte Entitäten, die eine strukturelle oder thrombotische Obliteration von kleinsten Hautgefäßen gemein haben. In diesem Artikel wird vor allem auf die Livedovaskulopathie mit und ohne Antiphospholipidsyndrom sowie die Calciphylaxie mit Abgrenzung zum Ulcus cruris hypertonicum als die relevantesten Differenzialdiagnosen von Unterschenkelulzerationen eingegangen. Der Begriff umfasst auch Gefäßverschlüsse, beispielsweise durch Oxalat oder Cholesterinembolie, sowie die septische Vaskulopathie. Diese führt häufig zu akralen Ulzerationen und stellt somit keine Differenzialdiagnose zum klassischen Ulcus cruris dar. Sie wird daher in diesem Artikel nicht behandelt.Der Verdacht einer okkludierenden Vaskulopathie kann über die typische Livedo racemosa beziehungsweise die (nichtinflammatorische) retiforme Purpura als Zeichen der kutanen Minderdurchblutung in der Wundumgebung gestellt werden. Entzündliche Dermatosen, insbesondere Vaskulitiden, müssen differenzialdiagnostisch abgegrenzt werden. Dies geschieht zum Teil klinisch und durch die histopathologische Beurteilung einer Gewebeprobe, die in ausreichender Größe und Tiefe sowie zum richtigen Zeitpunkt entnommen werden muss. Ergänzend können spezifische Laborparameter, insbesondere die Gerinnungsdiagnostik, die Diagnosestellung unterstützen.
S2k‐Leitlinie: Diagnostik und Therapie der zirkumskripten Sklerodermie
Authors: Kreuter Alexander , Moinzadeh Pia , Kinberger Maria , Horneff Gerd , Worm Margitta , Werner Ricardo N. , Hammacher Andreas , Krieg Thomas , Wenzel Jörg , Oeschger Michael , Weibel Lisa , Müllegger Robert , Hunzelmann Nicolas 
Published: April 2024

ZusammenfassungDie vorliegende aktualisierte S2k‐Leitlinie befasst sich mit der Diagnostik und der Therapie der zirkumskripten Sklerodermie (ZS). Diese umfasst ein Spektrum sklerotischer Erkrankungen der Haut mit, je nach Subtyp und Lokalisation, möglicher Beteiligung von hautnahen Strukturen wie Fettgewebe, Muskulatur, Gelenke und Knochen. Ein Befall innerer Organe oder ein Übergang in eine systemische Sklerodermie treten nicht auf. Eingeteilt werden kann die ZS in die vier Hauptformen der limitierten, generalisierten, linearen und gemischten Form. Teilweise existieren weitere Unterformen. Bei limitiertem Hautbefall empfiehlt die Leitlinie primär eine Therapie mit topischen Kortikosteroiden. Eine UV‐Therapie kann ebenfalls sinnvoll sein. Bei Subtypen mit schwerem Hautbefall oder muskuloskelettalem Befall wird eine systemische Therapie mit Methotrexat empfohlen. In der aktiven Phase der Erkrankung können ergänzend systemische Glukokortikosteroide eingesetzt werden. Bei MTX‐ und Steroid‐refraktären Verläufen, Kontraindikation oder Unverträglichkeit sollten Mycophenolat‐Mofetil, Mycophenolsäure oder Abatacept als Systemtherapie der zweiten Wahl eingesetzt werden. Bei einer linearen ZS kann zudem eine autologe Fettstammzelltransplantation zur Korrektur von Weichteildefekten erfolgen.