bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspective of US farmers on collaborative on‐farm agronomic research
Authors: Pires Carlos B. , Krupek Fernanda S. , Carmona Gabriela I. , Ortez Osler A. , Thompson Laura , Quinn Daniel J. , Reis Andre F. B. , Werle Rodrigo , Kovács Péter , Singh Maninder P. , Hutchinson J. M. Shawn , Ruiz Diaz Dorivar , Rice Charles W. , Ciampitti Ignacio A. 
Published: March 2024

AbstractOn‐farm research has emerged in recent years as a unique approach to involve farmers and other agricultural stakeholders as active participants in knowledge development and as an effective method of technology and innovation transfer across farms. This study assessed the perspective and knowledge of US farmers regarding on‐farm research via the implementation of a 24‐question survey distributed across most of the US Midwest and South‐Central regions. We found that farmers generally are willing to engage with universities to conduct on‐farm research and were 40% more likely to adopt practices supported by on‐farm research findings than research not conducted on‐farm. Notably, a shift toward conservation practices was made, with cover crops and no‐till at the forefront. Insights of this nature have implications for fostering collaborations, addressing constraints, and maximizing the impact of on‐farm research, offering guidance for sustainable agriculture progress in the United States and beyond. Results from this research survey could be used to initiate much‐needed policies to promote on‐farm research. Further, information on the benefits and drawbacks of on‐farm research could be used in the development of studies that benefit both farmers and researchers.
Copper‐Promoted Oxidative Amide‐Assisted Radical Selenylation of Anilides and N‐Arylsulfonamides with Diselenides
Authors: Shi Lou , Xu Penghui , Ma Yingchun , Dong Yongrui , Li Yanni , Liang Deqiang 
Published: March 2024

AbstractA direct selenylation of N‐arylsulfonamides and anilides using environmentally friendly and readily available copper promoted assisted by amide is presented. This method provided convenient access for various selenylation reactions, demonstrating a wide range of substrates and strong tolerance to various functional groups. According to the selection of reaction substrates, products can be isolated to obtain a regioselectivity ortho‐, para‐, or unprecedented ortho, para‐diselenylation products. Such transformation was elucidated by a proposed copper‐promoted amide‐assisted radical mechanism.
Sustainable Vat Photopolymerization‐Based 3D‐Printing through Dynamic Covalent Network Photopolymers
Authors: Pruksawan Sirawit , Chong Yi Ting , Zen Wylma , Loh Terence Jun En , Wang FuKe 
Published: March 2024

AbstractVat photopolymerization (VPP) based three‐dimensional (3D) printing, including stereolithography (SLA) and digital light projection (DLP), is known for producing intricate, high‐precision prototypes with superior mechanical properties. However, the challenge lies in the non‐recyclability of covalently crosslinked thermosets used in these printing processes, limiting the sustainable utilization of printed prototypes. This review paper examines the recently explored avenue of VPP 3D‐printed dynamic covalent network (DCN) polymers, which enable reversible crosslinks and allow for the reprocessing of printed prototypes, promoting sustainability. These reversible crosslinks facilitate the rearrangement of crosslinked polymers, providing printed polymers with chemical/physical recyclability, self‐healing capabilities, and degradability. While various mechanisms for DCN polymer systems are explored, this paper focuses solely on photocurable polymers to highlight their potential to revolutionize the sustainability of VPP 3D printing.
Origin of Performance Improvements in Lithium‐Ion Cells after Fast Formation
Authors: Witt Daniel , Bläubaum Lars , Baakes Florian , Krewer Ulrike 
Published: March 2024

AbstractThe formation process of lithium‐ion batteries commonly uses low current densities, which is time‐consuming and costly. Experimental studies have already shown that slow formation may neither be necessary nor beneficial for cell lifetime and performance. This work combines an experimental formation variation with physicochemical cell and solid electrolyte interphase (SEI) modeling to reveal formation‐induced changes within the cells. Formation at C/2 without full discharge compared to a standard C/10 formation at 20 °C notably improves the discharge and charge capacities at 2C by up to 41 % and 63 %, respectively, while reducing the formation time by over 80 %. Model‐based cell diagnostics reveal that these performance gains are driven by improved transport in the anode electrolyte phase, which is affected by SEI formation, and by enhanced transport on the cathode side. Hence, the focus on the dense SEI layer is insufficient for a comprehensive understanding and, ultimately, optimization of cell formation. All formation procedures were also tested at temperatures of 35 °C and 50 °C. Despite often surpassing the 2C discharge capacity of the standard formation at 20 °C, these cells showed comparable or lower 2C charge capacities. This suggests a pivotal role of local temperature in the formation of large‐format cells.
Extreme Outcomes Accentuate Overweighting of Low Probabilities and Underweighting of High Probabilities in Experience‐Based Choice
Authors: Elston Thomas W. , Leuthold Hartmut , Mackenzie Ian G. , Mittelstädt Victor 
Published: March 2024

ABSTRACTSubjective inferences of probability are critical for decisions involving uncertainty. Recent studies have suggested that extreme outcomes bias beliefs about the value of uncertain options toward the best/worst outcome possible when learning the odds through experience, leading to increased preferences for uncertain options over equivaluable sure bets when there is the prospect of gain and, conversely, aversion to uncertain options when there is the prospect of loss. However, prior studies regarding the influence of extreme outcomes on decisions involving uncertainty have only done so using 50/50 gambles, and it was unclear whether extreme outcomes biased probability perception more broadly. Across three pre‐registered experiments, we found that when people made decisions between equivaluable certain and uncertain options, they particularly preferred uncertain options at low probabilities (20%) when there was the prospect of gain and avoided them when there was the prospect of loss, with these preferences being reduced or even reversed at medium (50%) and high (80%) probabilities. We also found that uncertainty preferences were influenced by outcome extremity and the relative certainty associated with safe reference options. We conclude that extreme outcomes accentuate the overweighting of low probabilities and the underweighting of high probabilities in experience‐based choice.
A triphenylamine‐based carbohydrazide hydrazone fluorescent probe for selective detection of hypochlorite and sensing acidic gases
Authors: Shao Yuxin , Sun Jingxuan , Li Peng , Gao Chao‐Ying , Yang Yang 
Published: March 2024

AbstractHypochlorous acid and its hypochlorite are important reactive oxygen species in the body, and are involved in various physiological processes related to immunity; their rapid detection is of great significance. Here, we synthesized a fluorescent probe (TPAS) by condensation of 4‐(diphenylamino)benzaldehyde, carbohydrazide, and salicylaldehyde, which can be used for the detection of ClO− in water and sensing of acidic gas in its solid state. The probe showed strong selective recognition of ClO− in acetonitrile and good tolerance to interference ions. There were good linear responses between the intensity of absorbance and fluorescence and the amount of ClO−. The TPAS solid and its paper strips can emit red fluorescence when exposed to volatile acidic vapours. After being treated with NH3, the red fluorescence can be restored to yellow. The response process of TPAS to ClO− and acid gases was characterized using nuclear magnetic resonance, electrospray ionisation mass spectrometry, transmission electron microscopy, and density functional theory calculations. Furthermore, it can be utilized in analyzing ClO− in commercially available bleaching products; the detection results were basically compatible with the labelled values. In addition, the probe is biocompatible and can be applied for imaging ClO− in zebrafish.
Proteomic workflows for deep phenotypic profiling of 3D organotypic liver models
Authors: Koutsilieri Stefania , Mickols Evgeniya , Végvári Ákos , Lauschke Volker M. 
Published: March 2024

AbstractOrganotypic human tissue models constitute promising systems to facilitate drug discovery and development. They allow to maintain native cellular phenotypes and functions, which enables long‐term pharmacokinetic and toxicity studies, as well as phenotypic screening. To trace relevant phenotypic changes back to specific targets or signaling pathways, comprehensive proteomic profiling is the gold‐standard. A multitude of proteomic workflows have been applied on 3D tissue models to quantify their molecular phenotypes; however, their impact on analytical results and biological conclusions in this context has not been evaluated. The performance of twelve mass spectrometry‐based global proteomic workflows that differed in the amount of cellular input, lysis protocols and quantification methods was compared for the analysis of primary human liver spheroids. Results differed majorly between protocols in the total number and subcellular compartment bias of identified proteins, which is particularly relevant for the reliable quantification of transporters and drug metabolizing enzymes. Using a model of metabolic dysfunction‐associated steatotic liver disease, we furthermore show that critical disease pathways are robustly identified using a standardized high throughput‐compatible workflow based on thermal lysis, even using only individual spheroids (1500 cells) as input. The results increase the applicability of proteomic profiling to phenotypic screens in organotypic microtissues and provide a scalable platform for deep phenotyping from limited biological material.
N6‐methyladenosine reader hnRNPA2B1 recognizes and stabilizes NEAT1 to confer chemoresistance in gastric cancer
Authors: Wang Jiayao , Zhang Jiehao , Liu Hao , Meng Lingnan , Gao Xianchun , Zhao Yihan , Wang Chen , Gao Xiaoliang , Fan Ahui , Cao Tianyu , Fan Daiming , Zhao Xiaodi , Lu Yuanyuan 
Published: March 2024

AbstractBackgroundChemoresistance is a major cause of treatment failure in gastric cancer (GC). Heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) is an N6‐methyladenosine (m6A)‐binding protein involved in a variety of cancers. However, whether m6A modification and hnRNPA2B1 play a role in GC chemoresistance is largely unknown. In this study, we aimed to investigate the role of hnRNPA2B1 and the downstream mechanism in GC chemoresistance.MethodsThe expression of hnRNPA2B1 among public datasets were analyzed and validated by quantitative PCR (qPCR), Western blotting, immunofluorescence, and immunohistochemical staining. The biological functions of hnRNPA2B1 in GC chemoresistance were investigated both in vitro and in vivo. RNA sequencing, methylated RNA immunoprecipitation, RNA immunoprecipitation, and RNA stability assay were performed to assess the association between hnRNPA2B1 and the binding RNA. The role of hnRNPA2B1 in maintenance of GC stemness was evaluated by bioinformatic analysis, qPCR, Western blotting, immunofluorescence, and sphere formation assays. The expression patterns of hnRNPA2B1 and downstream regulators in GC specimens from patients who received adjuvant chemotherapy were analyzed by RNAscope and multiplex immunohistochemistry.ResultsElevated expression of hnRNPA2B1 was found in GC cells and tissues, especially in multidrug‐resistant (MDR) GC cell lines. The expression of hnRNPA2B1 was associated with poor outcomes of GC patients, especially in those who received 5‐fluorouracil treatment. Silencing hnRNPA2B1 effectively sensitized GC cells to chemotherapy by inhibiting cell proliferation and inducing apoptosis both in vitro and in vivo. Mechanically, hnRNPA2B1 interacted with and stabilized long noncoding RNA NEAT1 in an m6A‐dependent manner. Furthermore, hnRNPA2B1 and NEAT1 worked together to enhance the stemness properties of GC cells via Wnt/β‐catenin signaling pathway. In clinical specimens from GC patients subjected to chemotherapy, the expression levels of hnRNPA2B1, NEAT1, CD133, and CD44 were markedly elevated in non‐responders compared with responders.ConclusionOur findings indicated that hnRNPA2B1 interacts with and stabilizes lncRNA NEAT1, which contribute to the maintenance of stemness property via Wnt/β‐catenin pathway and exacerbate chemoresistance in GC.
Climbing into their Skin to Understand Contextual Protein–Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms
Authors: Long Marcus J. C. , Aye Yimon 
Published: March 2024

AbstractBorrowing some quotes from Harper Lee's novel “To Kill A Mockingbird” to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer proteins than biotinylation methods, their ability to probe simultaneously protein function and locale raises new and interesting possibilities for the field.
Morel‐Lavallée lesion of the lumbosacral region: A rare case report and comprehensive review of the literature
Authors: Lamichhane Samiksha , Devkota Shritik , K.C Suraj , Sarmast Arif Hussain 
Published: March 2024

Key Clinical MessageThe presentation of a Morel‐Lavallée lesion in the lumbosacral region following a road traffic accident is an uncommon clinical entity. Knowledge of this rare site of occurrence can help in early diagnosis and proper management.
On the Thermodynamic Equivalence of Grand Canonical, Infinite‐Size, and Capacitor‐Based Models in First‐Principle Electrochemistry
Authors: Kastlunger Georg , Vijay Sudarshan , Chen Xi , Sharma Shubham , Peterson Andrew 
Published: March 2024

AbstractFirst principles‐based computational and theoretical methods are constantly evolving trying to overcome the many obstacles towards a comprehensive understanding of electrochemical processes on an atomistic level. One of the major challenges has been the determination of reaction energetics under a constant potential. Here, a theoretical framework was proposed applying standard electronic structure methods and extrapolating to the infinite‐cell size limit where reactions do not alter the potential. Today, electronically grand canonical modifications to electronic structure methods, holding the potential constant by varying the number of electrons in a finite simulation cell, become increasingly popular. In this perspective, we show that these two schemes are thermodynamically equivalent. Further, we link these methods to capacitive models of the interface, in the limit that the capacitance of the charging components (whether continuum or atomistic) are equal and invariant along the reaction pathway.We benchmark the three approaches with an example of alkali cation adsorption on Pt(111) showing that all three approaches converge in the cases of Li, Na and K. For Cs, however, strong deviation from the ideal conditions leads to a spread in the respective results. We discuss the latter by highlighting the cases of broken equivalence and assumptions among the approaches.
dTBI2: A Calibrated, Tunable Device for Administering Traumatic Brain Injury in Drosophila
Authors: Ratner Stephen W. , Fetchko Michael , Mathivanan Akanksha S. , Kelly Seanna E. , Gupta Shambhavi , Barber Annika F. 
Published: March 2024

AbstractThe second‐generation Drosophila traumatic brain injury (TBI) device dTBI2 improves Drosophila TBI administration by providing a moderate‐throughput, tunable, head‐specific injury. Our updated device design improves user‐friendliness, eliminates inconsistencies in injury timing, and has an updated circuit design to extend the longevity of delicate electronic components. dTBI2 improves reproducibility across users and runs, and results in more consistent post‐injury phenotypes. This protocol describes the construction, calibration, and use of the dTBI2 device, which uses an Arduino‐controlled piezoelectric actuator to deliver a force that compresses a fly head against a metal collar. The duration and depth of head compression is tunable, allowing calibration of injury severity. All device components are commercially available, and the entire device can be constructed in under a week for less than $1000. The dTBI2 design will enable any lab to build a highly reliable, low‐cost device for Drosophila TBI, facilitating increased adoption and ease of exploration of closed‐head TBI in Drosophila for forward genetic screens. We describe below the three protocols necessary for constructing a dTBI2 device. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Construction of the dTBI2 control deviceBasic Protocol 2: Construction of the piezoelectric actuator housingBasic Protocol 3: Administration of dTBI2 injuries
Pollen‐mediated gene flow from herbicide‐resistant yellow corn to non‐genetically engineered food‐grade white corn
Authors: Singh Mandeep , Kumar Vipan , Knezevic Stevan Z. , Lindquist John L. , Irmak Suat , Pitla Santosh , Jhala Amit J. 
Published: March 2024

AbstractNebraska is the number one producer of food‐grade white corn in the United States. Pollen‐mediated gene flow (PMGF) from genetically engineered high alpha‐amylase corn, known as Enogen corn, to food‐grade white corn can have undesirable outcomes. Alpha‐amylase can convert starch in white corn to sugar during or after its processing, degrading the quality of processed products. Thus, proximity to Enogen corn puts white corn production at risk. The objectives of this study were to evaluate the PMGF from herbicide‐resistant yellow corn to food‐grade white corn and assess the significance of wind speed and direction. Field experiments were set up using a Nelder‐wheel design in 2021 and 2022 in Nebraska, with yellow field corn as pollen donor in the center and white corn surrounding it as pollen receptor. At the end of the season, samples of white corn cobs were collected up to 50 and 70 m from four cardinal and ordinal directions, respectively. PMGF was detected by counting the number of yellow kernels on the white cobs. More than 4 million kernels were screened and the highest frequency of PMGF (0.0621–0.1950) was detected at the nearest distance (1 m). PMGF decreased exponentially with distance; however, it was still observed (0.0020–0.0032) at the greatest distance (70 m) evaluated in this study. Wind profile played a significant role in PMGF. Wind frequency (r = 0.58 ≤ 0.86) and wind run (r = 0.36 ≤ 0.95) were moderately to strongly correlated with PMGF in 2021 and 2022, respectively. The results are concerning for white corn growers due to the coexistence of Enogen corn and food‐grade white corn.
Leveraging Direct Pyrolysis for the Synthesis of 10 nm Monodispersed Fe3O4/Fe3C NPS@Carbon to Improve SupercapacitANCE in Acidic Electrolyte
Authors: Appiah‐Ntiamoah Richard , Kim Hern 
Published: March 2024

AbstractThe prevailing practice advocates pre–oxidation of electrospun Fe–salt/polymer nanofibers (Fe–salt/polymer Nf) before pyrolysis as advantageous in the production of high–performance FeOx@carbon nanofibers supercapacitors (FeOx@C). However, our study systematically challenges this notion by demonstrating that pre–oxidation facilitates the formation of polydispersed and large FeOx nanoparticles (FeOx@CI−DA) through “external” Fe3+ Kirkendall diffusion from carbon, resulting in subpar electrochemical properties. To address this, direct pyrolysis of Fe–salt/polymer Nf is proposed, promoting “internal” Fe3+ Kirkendall diffusion within carbon and providing substantial physical confinement, leading to the formation of monodispersed and small FeOx nanoparticles (FeOx@CDA). In 1 M H2SO4, FeOx@CDA demonstrates ~2.60× and 1.26× faster SO42− diffusivity, and electron transfer kinetics, respectively, compared to FeOx@CI−DA, with a correspondingly ~1.50× greater effective surface area. Consequently, FeOx@CDA exhibits a specific capacity of 161.92 mAhg−1, ~2× higher than FeOx@CI−DA, with a rate capability ~19 % greater. Moreover, FeOx@CDA retains 94 % of its capacitance after 5000 GCD cycles, delivering an energy density of 26.68 Whkg−1 in a FeOx@CDA//FeOx@CDA device, rivaling state–of–the–art FeOx/carbon electrodes in less Fe–corrosive electrolytes. However, it is worth noting that the effectiveness of direct pyrolysis is contingent upon hydrated Fe–salt. These findings reveal a straightforward approach to enhancing the supercapacitance of FeOx@C materials.
Genomic analyses elucidate S‐locus evolution in response to intra‐specific losses of distyly in Primula vulgaris
Authors: Mora‐Carrera E. , Stubbs R. L. , Potente G. , Yousefi N. , Keller B. , de Vos J. M. , Szövényi P. , Conti E. 
Published: March 2024

AbstractDistyly, a floral dimorphism that promotes outcrossing, is controlled by a hemizygous genomic region known as the S‐locus. Disruptions of genes within the S‐locus are responsible for the loss of distyly and the emergence of homostyly, a floral monomorphism that favors selfing. Using whole‐genome resequencing data of distylous and homostylous individuals from populations of Primula vulgaris and leveraging high‐quality reference genomes of Primula we tested, for the first time, predictions about the evolutionary consequences of transitions to selfing on S‐genes. Our results reveal a previously undetected structural rearrangement in CYPᵀ associated with the shift to homostyly and confirm previously reported, homostyle‐specific, loss‐of‐function mutations in the exons of the S‐gene CYPᵀ. We also discovered that the promoter and intronic regions of CYPᵀ in distylous and homostylous individuals are conserved, suggesting that down‐regulation of CYPᵀ via mutations in its promoter and intronic regions is not a cause of the shift to homostyly. Furthermore, we found that hemizygosity is associated with reduced genetic diversity in S‐genes compared with their paralogs outside the S‐locus. Additionally, the shift to homostyly lowers genetic diversity in both the S‐genes and their paralogs, as expected in primarily selfing plants. Finally, we tested, for the first time, long‐standing theoretical models of changes in S‐locus genotypes during early stages of the transition to homostyly, supporting the assumption that two copies of the S‐locus might reduce homostyle fitness.