bluuebunny/crossref_metadata_2025_split huggingface dataset:
Behavioral responses and life history traits of Taiwanese and Indonesian populations of Aedes aegypti surviving deltamethrin–clothianidin treatment
Authors: Silalahi Christina Natalina , Yasin Aqsa , Chen Mei-Er , Ahmad Intan , Neoh Kok-Boon 
Published: March 2024

Abstract
Background
Indoor residual spraying (IRS) capitalizes on the natural behavior of mosquitoes because Aedes aegypti commonly seeks indoor resting sites after a blood meal. This behavior allows mosquitoes to be exposed to insecticide-treated surfaces and subsequently killed. Combinations of deltamethrin and clothianidin with different modes of action have shown promise in IRS, effectively targeting both susceptible and pyrethroid-resistant malaria vectors. However, the effects of this approach on Aedes mosquitoes remain unclear. The present study tested the effects of deltamethrin–clothianidin mixture treatment on behavioral responses and life history traits of Taiwanese and Indonesian populations of Ae. aegypti.

Methods
We adopted an excito-repellent approach to explore the behavioral responses of pyrethroid-resistant Ae. aegypti populations from Indonesia and Taiwan to a deltamethrin–clothianidin mixture used in contact irritancy and non-contact repellency treatments. We further evaluated the life history traits of surviving mosquitoes (i.e., delayed mortality after 7-day post-treatment, longevity, fecundity, and egg hatching) and investigated the potential transgenerational hormetic effects of insecticide exposure (i.e., development rate and survival of immatures and adult mosquitos).

Results
All tested field populations of Ae. aegypti displayed strong contact irritancy responses; the percentage of escape upon insecticide exposure ranged from 38.8% to 84.7%. However, repellent effects were limited, with the escape percentage ranging from 4.3% to 48.9%. We did not observe immediate knockdown or mortality after 24 h, and less than 15% of the mosquitoes exhibited delayed mortality after a 7-day exposure period. However, the carryover effects of insecticide exposure on the survival of immature mosquitoes resulted in approximately 25% higher immature mortality than that in the control. By contrast, we further documented stimulated survivor reproduction and accelerated transgenerational immature development resulting from the sublethal effects of the insecticide mixture. In particular, the number of eggs laid by treated (both treatments) female mosquitoes increased by at least 60% compared with that of eggs laid by control female mosquitoes.

Conclusions
IRS with deltamethrin–clothianidin effectively deters Aedes mosquitoes from entering residential areas and thereby reduces mosquito bites. However, the application rate (deltamethrin: 25 mg/m2; clothianidin: 200 mg/m2) may be insufficient to effectively kill Aedes mosquitoes. Insecticide response appears to vary across mosquito species; their behavioral and physiological responses to sublethal doses have crucial implications for mosquito control programs.

Graphical Abstract
Gastrosplenic fistula due to splenic lymphoma: two case reports and review of the literature
Authors: Ksontini Feryel Letaief , Zaimi Yosra , Nefzi Isaad , Khrouf Salim , Ayari Myriam , Sghaier Sonia , Zidi Asma , Magherbi Houcine , Ayadi Mouna 
Published: March 2024

Abstract
Background
Gastrosplenic fistula is a rare and potentially fatal complication of various conditions. Lymphoma is the most common cause. It can occur spontaneously or after chemotherapy. Gastrosplenic fistula diagnosis can be confused with a splenic abscess because of the presence of air into the mass. The computed tomography identification of the fistulous tract is the key to a right diagnosis. Treatment modalities include surgical resection, chemotherapy, or a combination of both.

Case presentation
Here we report two patients with gastrosplenic fistula due to diffuse large B cell lymphoma. The first patient was a 54-year-old Caucasian woman with an enormous primary splenic diffuse large B cell lymphoma leading to the development of a spontaneous fistula in the stomach. The second patient was a 48-year-old Caucasian male patient with an enormous splenic diffuse large B cell lymphoma complicated by fistula after chemotherapy. Both patients died of septic shock several days after surgery.

Conclusion
Gastrosplenic fistula is a rare complication with a poor-prognosis, for which surgery is currently the preferred treatment.
Metabolic signatures and potential biomarkers of sarcopenia in suburb-dwelling older Chinese: based on untargeted GC–MS and LC–MS
Authors: Han Peipei , Yuan Chunhua , Chen Xiaoyu , Hu Yuanqing , Hu Xiaodan , Xu Zhangtao , Guo Qi 
Published: March 2024

Abstract
Background
Untargeted metabolomics can be used to expand our understanding of the pathogenesis of sarcopenia. However, the metabolic signatures of sarcopenia patients have not been thoroughly investigated. Herein, we explored metabolites associated with sarcopenia by untargeted gas chromatography (GC)/liquid chromatography (LC)–mass spectrometry (MS) and identified possible diagnostic markers.

Methods
Forty-eight elderly subjects with sarcopenia were age and sex matched with 48 elderly subjects without sarcopenia. We first used untargeted GC/LC–MS to analyze the plasma of these participants and then combined it with a large number of multivariate statistical analyses to analyze the data. Finally, based on a multidimensional analysis of the metabolites, the most critical metabolites were considered to be biomarkers of sarcopenia.

Results
According to variable importance in the project (VIP > 1) and the p-value of t-test (p < 0.05), a total of 55 metabolites by GC–MS and 85 metabolites by LC–MS were identified between sarcopenia subjects and normal controls, and these were mostly lipids and lipid-like molecules. Among the top 20 metabolites, seven phosphatidylcholines, seven lysophosphatidylcholines (LysoPCs), phosphatidylinositol, sphingomyelin, palmitamide, L-2-amino-3-oxobutanoic acid, and palmitic acid were downregulated in the sarcopenia group; only ethylamine was upregulated. Among that, three metabolites of LysoPC(17:0), L-2-amino-3-oxobutanoic acid, and palmitic acid showed very good prediction capacity with AUCs of 0.887 (95% CI = 0.817–0.957), 0.836 (95% CI = 0.751–0.921), and 0.805 (95% CI = 0.717–0.893), respectively.

Conclusions
These findings show that metabonomic analysis has great potential to be applied to sarcopenia. The identified metabolites could be potential biomarkers and could be used to study sarcopenia pathomechanisms.
RNA-RBP interactions recognition using multi-label learning and feature attention allocation
Authors: Han Huirui , Talpur Bandeh Ali , Liu Wei , Wang Limei , Ahmed Bilal , Sarhan Nadia , Awwad Emad Mahrous 
Published: March 2024

AbstractIn this study, we present a sophisticated multi-label deep learning framework for the prediction of RNA-RBP (RNA-binding protein) interactions, a critical aspect in understanding RNA functionality modulation and its implications in disease pathogenesis. Our approach leverages machine learning to develop a rapid and cost-efficient predictive model for these interactions. The proposed model captures the complex characteristics of RNA and recognizes corresponding RBPs through its dual-module architecture. The first module employs convolutional neural networks (CNNs) for intricate feature extraction from RNA sequences, enabling the model to discern nuanced patterns and attributes. The second module is a multi-view multi-label classification system incorporating a feature attention mechanism. The second module is a multi-view multi-label classification system that utilizes a feature attention mechanism. This mechanism is designed to intricately analyze and distinguish between common and unique deep features derived from the diverse RNA characteristics. To evaluate the model's efficacy, extensive experiments were conducted on a comprehensive RNA-RBP interaction dataset. The results emphasize substantial improvements in the model's ability to predict RNA-RBP interactions compared to existing methodologies. This advancement emphasizes the model's potential in contributing to the understanding of RNA-mediated biological processes and disease etiology.
Macroalgal microbiomes unveil a valuable genetic resource for halogen metabolism
Authors: Lavecchia Anna , Fosso Bruno , Engelen Aschwin H. , Borin Sara , Manzari Caterina , Picardi Ernesto , Pesole Graziano , Placido Antonio 
Published: March 2024

Abstract
Background
Macroalgae, especially reds (Rhodophyta Division) and browns (Phaeophyta Division), are known for producing various halogenated compounds. Yet, the reasons underlying their production and the fate of these metabolites remain largely unknown. Some theories suggest their potential antimicrobial activity and involvement in interactions between macroalgae and prokaryotes. However, detailed investigations are currently missing on how the genetic information of prokaryotic communities associated with macroalgae may influence the fate of organohalogenated molecules.

Results
To address this challenge, we created a specialized dataset containing 161 enzymes, each with a complete enzyme commission number, known to be involved in halogen metabolism. This dataset served as a reference to annotate the corresponding genes encoded in both the metagenomic contigs and 98 metagenome-assembled genomes (MAGs) obtained from the microbiome of 2 red (Sphaerococcus coronopifolius and Asparagopsis taxiformis) and 1 brown (Halopteris scoparia) macroalgae. We detected many dehalogenation-related genes, particularly those with hydrolytic functions, suggesting their potential involvement in the degradation of a wide spectrum of halocarbons and haloaromatic molecules, including anthropogenic compounds. We uncovered an array of degradative gene functions within MAGs, spanning various bacterial orders such as Rhodobacterales, Rhizobiales, Caulobacterales, Geminicoccales, Sphingomonadales, Granulosicoccales, Microtrichales, and Pseudomonadales. Less abundant than degradative functions, we also uncovered genes associated with the biosynthesis of halogenated antimicrobial compounds and metabolites.

Conclusion
The functional data provided here contribute to understanding the still largely unexplored role of unknown prokaryotes. These findings support the hypothesis that macroalgae function as holobionts, where the metabolism of halogenated compounds might play a role in symbiogenesis and act as a possible defense mechanism against environmental chemical stressors. Furthermore, bacterial groups, previously never connected with organohalogen metabolism, e.g., Caulobacterales, Geminicoccales, Granulosicoccales, and Microtrichales, functionally characterized through MAGs reconstruction, revealed a biotechnologically relevant gene content, useful in synthetic biology, and bioprospecting applications.
New material connections in a mother-of-pearl Enconchado from the Viceroyalty of New Spain
Authors: Dismukes Avalon H. , Lazarte José L. , Centeno Silvia A. 
Published: March 2024

Abstract

Adoration of the Magi (ca. 1695–1700), an enconchado painting attributed to Miguel González, active in Mexico in the late seventeenth century, exemplifies the refinement of the arts produced in Spanish America as a response to the taste for Asian goods during the seventeenth and eighteenth centuries. The painting and its original mother-of-pearl inlaid frame were examined and analyzed using non-invasive and micro-sampling methods, an approach that permitted answering outstanding questions about the stratigraphy, pigments, and the use of shells throughout the painting and frame. The identification of the mother-of-pearl, determined to be from a Pinctada species, was a central focus of this study. In addition to the inlaid shells in the painting and frame, shell fragments were observed mixed with gypsum in the ground preparation of the painting by SEM–EDS. To our knowledge, this is a novel identification of marine shells in the ground of an easel painting. Traces of workmanship in the inlaid shells observed by SEM and optical microscopy are possibly connected to pre-Hispanic methodologies of mother-of-pearl refinement.

Graphical Abstract
Sustainable threads: analyzing the impact of corporate social performance, CSR message authenticity and information transparency on purchase intentions
Authors: Jung Hye Jung , Tian Xinyu , Oh Kyung Wha 
Published: March 2024

AbstractWith the deepening exploration of the study on corporate social responsibility (CSR), CSR has progressively gained significance for enterprises. This study aims to analyze the impact of fashion companies’ information disclosure on the intention of Chinese consumers who purchase clothing by providing information related to the environment, suppliers, manufacturing processes, and pricing. An experimental study was conducted using stimuli on 412 consumers in Shandong Province, China. The research results are as follows. First, it was found that consumers’ perception of Corporate Social Performance (CSP) has a positive effect on the authenticity of Corporate Social Responsibility (CSR) message and consumers’ subjective norms. Second, CSR message authenticity and consumer subjective norms mediate the perception of CSP in the process of leading to purchasing activities. Third, consumers perceive the authenticity of a company’s CSR activities according to the degree of transparent information disclosure of the cost and manufacturing process of its products. This study shows that the CSR information provided by the company is authentic depending on the degree of CSR information transparency of the company, and that it leads to a purchase intention when consumers judge that the company’s CSR activities are authentic. Accordingly, it is suggested that many fashion companies’ CSR activities lead to product purchases when they are continuously conducted on environmental, social problems, and transparency in the production process.
Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system
Authors: Zhang Xiaokang , Zhang Zhanquan , Chen Tong , Chen Yong , Li Boqiang , Tian Shiping 
Published: March 2024

AbstractBotrytis cinerea is one of the most destructive phytopathogenic fungi, causing significant losses to horticultural crops. As a necrotrophic fungus, B. cinerea obtains nutrients by killing host cells. Secreted cell death-inducing proteins (CDIPs) play a crucial role in necrotrophic infection; however, only a limited number have been reported. For high-throughput CDIP screening, we optimized the prokaryotic expression system and compared its efficiency with other commonly used protein expression systems. The optimized prokaryotic expression system showed superior effectiveness and efficiency and was selected for subsequent CDIP screening. The screening system verified fifty-five candidate proteins and identified two novel SGNH family CDIPs: BcRAE and BcFAT. BcRAE and BcFAT exhibited high expression levels throughout the infection process. Site-directed mutagenesis targeting conserved Ser residues abolished the cell death-inducing activity of both BcRAE and BcFAT. Moreover, the transient expression of BcRAE and BcFAT in plants enhanced plant resistance against B. cinerea without inducing cell death, independent of their enzymatic activities. Our results suggest a high-efficiency screening system for high-throughput CDIP screening and provide new targets for further study of B. cinerea-plant interactions.
Clinical outcomes with paliperidone palmitate 3-monthly injection as monotherapy: observational 3-year follow-up of patients with schizophrenia
Authors: Clark Ivana , Wallman Phoebe , Gee Siobhan , Taylor David 
Published: March 2024

Abstract

Background
Paliperidone palmitate 3-monthly (PP3M) has been tested in 1-year controlled studies. The aim of this study was to examine the relapse outcomes with PP3M monotherapy at 3 years in patients with schizophrenia.

Methods
This was an observational, non-interventional study of patients started on PP3M according to their clinical need. All patients had a diagnosis of schizophrenia (ICD-10 F20) and were between 18 and 65 years of age. The study took place in a mental health facility in South East London, UK.

Results
Among the 166 patients who started PP3M, 97 (58%) met inclusion criteria and were observed for 36 months. In total, five patients (5%) experienced a relapse (defined as step-up in clinical care) while on PP3M. There were no relapses between months 18 and 36. Of the original 97 patients, 56 (58%) remained on PP3M monotherapy at 3 years, and 71 (73%) remained on either PP3M or paliperidone palmitate one-monthly. Reasons for discontinuation of PP3M included patient refusal (n = 11, 33% of discontinuations) and adverse effects in (n = 8, 24%).

Conclusion
PP3M is a highly effective monotherapy treatment for reducing relapse in people with schizophrenia.
Cost-Effectiveness Analysis of Denosumab in the Prevention of Skeletal-Related Events Among Patients With Breast Cancer With Bone Metastasis in India
Authors: Wadhwa Raina , Gupta Nidhi , Dixit Jyoti , Malhotra Pankaj , Lakshmi PVM , Prinja Shankar 
Published: March 2024

PURPOSEDenosumab is clinically superior to zoledronic acid (ZA) for preventing and delaying time to first and subsequent skeletal-related events (SREs) among patients with breast cancer (BC) with bone metastases. We evaluated the cost and health benefits of denosumab and ZA (once every 4 weeks and once every 12 weeks) among four different molecular subtypes of BC with bone metastases in India.MATERIALS AND METHODSA Markov model was developed in Microsoft Excel to estimate lifetime health consequences and resulting costs among cohort of 1,000 patients with BC with bone metastasis, for three intervention scenarios, namely denosumab (once every 4 weeks), ZA (once every 4 weeks), and ZA (once every 12 weeks). The health outcomes were measured in terms of SREs averted and quality-adjusted life-years (QALYs) gained. The cost of each intervention scenario was measured using both the health system and the patient's perspectives. Indirect costs because of lost productivity were not included. The future costs and outcomes were discounted at the standard rate of 3%.RESULTSOver a lifetime, the incremental number of SREs averted with use of denosumab once every 4 weeks (compared with ZA once every 4 weeks and once every 12 weeks) among patients with luminal A, luminal B, human epidermal growth factor receptor 2–enriched, and triple negative breast cancer were estimated as 0.39, 0.26, 0.25, and 0.19, respectively. The number of QALYs lived were slightly higher in the denosumab arm (1.45-2.80) compared with ZA once every 4 weeks and once every 12 weeks arms (1.44-2.78). However, denosumab once every 4 weeks was not found to be a cost-effective alternative for either of the four molecular subtypes of breast cancer. ZA once every 12 weeks was found to be a cost-effective option with an average cost-effectiveness ratio ranging between ₹68,254 and ₹73,636.CONCLUSIONZA once every 12 weeks is the cost-effective treatment option for BC with bone metastases in India. The present study findings hold significance for standard treatment guidelines under India’s government-funded health insurance program.
TARGET: A Randomized, Noninferiority Trial of a Pretest, Patient-Driven Genetic Education Webtool Versus Genetic Counseling for Prostate Cancer Germline Testing
Authors: Loeb Stacy , Keith Scott W. , Cheng Heather H. , Leader Amy E. , Gross Laura , Sanchez Nolasco Tatiana , Byrne Nataliya , Hartman Rebecca , Brown Lauren H. , Pieczonka Christopher Michael , Gomella Leonard G. , Kelly William Kevin , Lallas Costas D. , Handley Nathan , Mille Patrick Johnston , Mark James Ryan , Brown Gordon Andrew , Chopra Sameer , McClellan Alexandra , Wise David R. , Hollifield Lucas , Giri Veda N. 
Published: March 2024

PURPOSE Germline genetic testing (GT) is important for prostate cancer (PCA) management, clinical trial eligibility, and hereditary cancer risk. However, GT is underutilized and there is a shortage of genetic counselors. To address these gaps, a patient-driven, pretest genetic education webtool was designed and studied compared with traditional genetic counseling (GC) to inform strategies for expanding access to genetic services. METHODS Technology-enhanced acceleration of germline evaluation for therapy (TARGET) was a multicenter, noninferiority, randomized trial (ClinicalTrials.gov identifier: NCT04447703 ) comparing a nine-module patient-driven genetic education webtool versus pretest GC. Participants completed surveys measuring decisional conflict, satisfaction, and attitudes toward GT at baseline, after pretest education/counseling, and after GT result disclosure. The primary end point was noninferiority in reducing decisional conflict between webtool and GC using the validated Decisional Conflict Scale. Mixed-effects regression modeling was used to compare decisional conflict between groups. Participants opting for GT received a 51-gene panel, with results delivered to participants and their providers. RESULTS The analytic data set includes primary outcome data from 315 participants (GC [n = 162] and webtool [n = 153]). Mean difference in decisional conflict score changes between groups was –0.04 (one-sided 95% CI, –∞ to 2.54; P = .01), suggesting the patient-driven webtool was noninferior to GC. Overall, 145 (89.5%) GC and 120 (78.4%) in the webtool arm underwent GT, with pathogenic variants in 15.8% (8.7% in PCA genes). Satisfaction did not differ significantly between arms; knowledge of cancer genetics was higher but attitudes toward GT were less favorable in the webtool arm. CONCLUSION The results of the TARGET study support the use of patient-driven digital webtools for expanding access to pretest genetic education for PCA GT. Further studies to optimize patient experience and evaluate them in diverse patient populations are warranted.
Taxonomy of an Enslaved Heart
Authors: Goldberg Shari 
Published: March 2024

Abstract“Taxonomy of an Enslaved Heart” opens up the figuration of heartache, so common to sentimental writings, to consider how it can signify anatomical pain as well. What does it mean to read figuratively—accepting that every instance of a heart broken or throbbing or heavy indexes emotional pain addressing the reader’s sympathy—and, at the same time, to literalize these instances, so that each one refers to a specific episode in the history of a circulatory system? This essay attempts to hold both in tension, even as they resist each other. Attending to texts by Harriet Jacobs, Mary Prince, Sojourner Truth, and James Baldwin, the essay argues for what it calls the story of the heart: a minoritized account of pain that deforms sentimental language to register at once somatically, mentally, and intersubjectively. Because of its insecure legibility, the story of the heart subverts the biopolitical logic of legitimacy that traps many patients who are Black, disabled, or both today. What emerges from holding figuration with literalization subtly shifts the illnesses we know and the conditions by which we know them.