bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colorectal cancer survival in the Nordic countries and the United Kingdom: Excess mortality risk analysis of 5 year relative period survival in the period 1999 to 2000
Authors: Engholm Gerda , Kejs Anne Mette T. , Brewster David H. , Gaard Maria , Holmberg Lars , Hartley Roger , Iddenden Robert , Møller Henrik , Sankila Risto , Thomson Catherine S. , Storm Hans H. 
Published: April 2007

AbstractA deficit in colorectal cancer survival in Denmark and in the UK compared to Sweden, Norway and Finland was found in the EUROCARE studies. We set out to explore if these differences still exist. Patients diagnosed with colorectal cancer as their first invasive cancer at age 15–89 in the period 1994–2000 were identified using data from 11 cancer registries in the UK and from four Nordic countries. Five‐year relative period survival using deaths in 1999–2000 following cancers diagnosed in 1994–2000 was analysed with excess mortality risk modelling. Follow‐up time since diagnosis with age as an effect‐modifier in the first half year was the most important factor with the highest excess risk of death immediately after diagnosis and with higher age and decreasing with length of follow‐up. Variations between countries were bigger in the first half year following diagnosis than in the interval 0.5–5 years with about 30% higher risk in UK and Denmark. The differences between countries are still substantial and the order has not changed, even if the five year relative survival has improved since the EUROCARE studies. Patient management, diagnostics, and comorbidity likely explain the excess deaths in UK and Denmark during the first 6 months. The effect of stage and quality of management and treatment should be examined in population based studies with detailed patient information. Use of more detailed age‐intervals than conventionally applied in survival studies proved to be important in statistical modelling and is recommended for future studies. © 2007 Wiley‐Liss, Inc.
Distinct pharmacological properties of second generation HDAC inhibitors with the benzamide or hydroxamate head group
Authors: Beckers Thomas , Burkhardt Carmen , Wieland Heike , Gimmnich Petra , Ciossek Thomas , Maier Thomas , Sanders Karl 
Published: April 2007

AbstractAdvanced second generation inhibitors of histone deacetylases (HDAC) are currently used in clinical development. This study aimed at comparing the pharmacological properties of selected second generation HDAC inhibitors with the hydroxamate and benzamide head group, namely SAHA, LAQ824/LBH589, CI994, MS275 and MGCD0103. In biochemical assays using recombinant HDAC1, 3, 6 and 8 isoenzymes, SAHA and LAQ824/LBH589 behave as quite unselective HDAC inhibitors. In contrast, the benzamides CI994, MS275 and MGCD0103 are more selective, potent inhibitors of at least HDAC1 and HDAC3. All HDAC inhibitors induce histone H3 hyperacetylation, correlating with inhibition of proliferation, induction of cell differentiation and apoptosis. A broad cytotoxicity is seen across cell lines from different tumor entities with LAQ824/LBH589 being the most potent agents. The apoptosis inducing activity is evident in arrested and proliferating RKO colon cancer cells with inducible, heterologous p21waf1 expression, indicative for a cell‐cycle independent mode‐of‐action. Differentiation of MDA‐MB468 breast cancer cells is induced by benzamide and hydroxamate analogs. The reversibility of drug action was evaluated by pulse treatment of A549 lung cancer cells. Whereas paclitaxel induced irreversible cell cycle alterations already after 6 hr treatment, HDAC inhibitor action was retarded and irreversible after >16 hr treatment. Interestingly, pulse treatment was equally effective as continous treatment. Finally, the efficacy of LAQ824, SAHA and MS275 in A549 nude mice xenografts was comparable to that of paclitaxel at well tolerated doses. We conclude that despite a different HDAC isoenzyme inhibition profile, hydroxamate and benzamide analogs as studied display similar cellular profiles. © 2007 Wiley‐Liss, Inc.
Synthesis and characterization of radiopaque magnetic core‐shell nanoparticles for X‐ray imaging applications
Authors: Galperin Anna , Margel Shlomo 
Published: April 2007

AbstractRadiopaque magnetic γ‐Fe2O3/poly(2‐methacryloyloxyethyl(2,3,5‐triiodobenzoate)) core‐shell nanoparticles of narrow size distribution were prepared by emulsion polymerization of the iodinated monomer 2‐methacryloyloxyethyl(2,3,5‐triiodobenzoate) in the presence of maghemite (γ‐Fe2O3 nanoparticles coated with a dextran shell are commonly used as contrast agents for magnetic resonance imaging (MRI). The present nanoparticles have similar core‐shell structure substituting the dextran for the iodo polymer. These core‐shell nanoparticles may therefore be useful as imaging contrast agents to detect various pathogenic zones and to observe different disease states in both modes: X‐ray and MRI. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007
Development of an engineering autologous palatal mucosa‐like tissue for potential clinical applications
Authors: Luitaud C. , Laflamme C. , Semlali A. , Saidi S. , Grenier G. , Zakrzewski A. , Rouabhia M. 
Published: April 2007

AbstractThe goal of this study was to optimize key processes in recreating functional and viable palatal mucosa‐like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape®, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6‐day culture, using 105 fibroblasts and 106 epithelial cells, a well‐organized palatal mucosa‐like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16‐positive cells located in the supra‐basal layer. This palatal mucosa‐like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft‐tissue defects secondary to trauma, congenital defects, and acquired diseases. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007
Surface micro‐analyses of long‐term worn retrieved “Osteal™” alumina ceramic total hip replacement
Authors: Tateiwa Toshiyuki , Clarke Ian C , Pezzotti Giuseppe , Sedel Laurent , Kumakura Tsuyoshi , Shishido Takaaki , Yamamoto Kengo 
Published: April 2007

AbstractWe analyzed wear pattern of long‐term retrieved alumina‐alumina hip prostheses from Osteal™, which were implanted for 15–19 years. A comparison was carried out with our previous study of 17‐year Biolox™ alumina‐on‐alumina hip prostheses, (Shishido et al., J Biomed Mater Res B 2003;67:638–647) and all‐alumina total hip replacement run under microseparation simulator tests. Of particular interest was the occurrence of stripe wear in these first generation alumina ceramic bearings. Two balls of Osteal revealed only one stripe wear as did the respective liners on their rim areas. In these latter balls, the stripes were shallower than those previously observed in Biolox implants. A microscopic analysis of the bearing surface was carried out using scanning electron microscopy and fluorescence microprobe spectroscopy. On average, the Osteal retrievals had one grade lower wear than Biolox retrievals. Fluorescence microprobe maps showed that Biolox ball surfaces had higher compressive stress than the Osteal likely due to severe impingement and microseparation promoted by the bulky implant design. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007
Development of a 95/5 poly(L‐lactide‐co‐glycolide)/hydroxylapatite and β‐tricalcium phosphate scaffold as bone replacement material via selective laser sintering
Authors: Simpson Rebecca Louise , Wiria Florencia Edith , Amis Andrew A. , Chua Chee Kai , Leong Kah Fai , Hansen Ulrich N. , Chandrasekaran Margam , Lee Mun Wai 
Published: April 2007

Abstract95/5 Poly(L‐lactide‐co‐glycolide) was investigated for the role of a porous scaffold, using the selective laser sintering (SLS) fabrication process, with powder sizes of 50–125 and 125–250 μm. SLS parameters of laser power, laser scan speed, and part bed temperature were altered and the degree of sintering was assessed by scanning electron microscope. Composites of the 125–250 β‐tricalcium phosphate (CAMCERAM® II) were sintered, and SLS settings using 40 wt % CAMCERAM® II were optimized for further tests. Polymer thermal degradation during processing led to a reduction in number and weight averaged molecular weight of 9% and 12%, respectively. Compression tests using the optimized composite sintering parameters gave a Young's modulus, yield strength, and strain at 1% strain offset of 0.13 ± 0.03 GPa, 12.06 ± 2.53 MPa, and 11.39 ± 2.60%, respectively. Porosity was found to be 46.5 ± 1.39%. CT data was used to create an SLS model of a human fourth middle phalanx and a block with designed porosity was fabricated to illustrate the process capabilities. The results have shown that this composite and fabrication method has potential in the fabrication of porous scaffolds for bone tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008
Dextran‐sulfate‐adsorption of atherosclerotic lipoproteins from whole blood or separated plasma for lipid‐apheresis—Comparison of performance characteristics with DALI and lipidfiltration
Authors: Julius Ulrich , Parhofer Klaus G. , Heibges Andreas , Kurz Stefan , Klingel Reinhard , Geiss Hans‐Christian 
Published: April 2007

AbstractFor many years dextran sulfate adsorption (DSA) treatment of separated plasma has been an established technology for low‐density lipoprotein (LDL)‐elimination. Recently a system for the treatment of whole blood based on DSA was introduced into clinical practice. To further characterize DSA treatment of whole blood, the performance characteristics of both DSA modalities were compared at two investigational sites with two alternative LDL apheresis systems being already in routine clinical use. In prospective cross‐over design, DSA whole blood treatment was compared with a whole blood polyacrylate LDL adsorption system (DALI) in one study group. DSA for plasma treatment was compared with Lipidfiltration in cross‐over design in a second study group. In total, 12 patients on chronic LDL apheresis received 169 treatments. Six patients were treated twice with whole blood polyacrylate adsorption and twice with whole blood DSA. LDL‐cholesterol (74.9–78.0%) and lipoprotein (a) (72.1–73.3%) were reduced by both with equal efficacy. DSA achieved a significantly higher reduction rate of fibrinogen. Another six patients were treated eight times with DSA plasma adsorption followed by 16 Lipidfiltration treatments. LDL‐cholesterol (67.0–70.2%) and lipoprotein (a) (69.2–73.7%) were reduced by both with equal efficacy. Fibrinogen was eliminated more efficiently by Lipidfiltration (50.2 vs. 38.5%). DSA proved to be a safe and effective in both treatment modes, for plasma as well as for whole blood. At the discretion of the apheresis specialist, depending upon the status of national approval, DSA of whole blood complements the armamentarium of powerful modalities for extracorporeal elimination of atherosclerotic lipoproteins to meet specific individual, medical, or logistic needs. J. Clin. Apheresis., 2007 © 2007 Wiley‐Liss, Inc.
Constitutive collagenase‐1 synthesis through MAPK pathways is mediated, in part, by endogenous IL‐1α during fibrotic repair in corneal stroma
Authors: Jung Jae‐Chang , Huh Man‐Il , Fini M. Elizabeth 
Published: April 2007

AbstractCollagenase‐1 is a protease expressed by active fibroblasts that is involved in remodeling of the extracellular matrix (ECM). In this study, we characterize the intracellular signaling mechanism of collagenase‐1 production by IL‐1α in subcultured normal fibroblasts (NF) from uninjured normal corneas, compared to that in repair wound fibroblasts (WF). In NF, collagenase‐1 was induced specifically after the exogenous addition of IL‐1α via activation of ERK and p38MAPK. Collagenase‐1 expression was strongly suppressed upon treatment with either a MEK or p38MAPK inhibitor. In contrast, repair WF constitutively synthesized both IL‐1α and collagenase‐1. Combined treatment with both mitogen‐activated protein kinase (MAPK) inhibitors dramatically reduced collagenase‐1 synthesis, while individual MEK1 or p38 inhibitors weakly modulated the collagenase‐1 level. The results indicate that both pathways are crucial in the regulation of collagenase‐1 synthesis. Furthermore, an IL‐1α receptor antagonist (IL‐1ra) could not abolish constitutive collagenase‐1 synthesis, even at high doses, suggesting that other cytokines/factors are additionally involved in this process. We propose that induction of collagenase‐1 by IL‐1α in both WF and NF depends on a unique combination of cell type‐specific signaling pathways. J. Cell. Biochem. 102: 453–462, 2007. © 2007 Wiley‐Liss, Inc.
RIF‐1, a novel nuclear receptor corepressor that associates with the nuclear matrix
Authors: Li Hui Joyce , Haque Zaffar K. , Chen Amy , Mendelsohn Michael 
Published: April 2007

AbstractThe retinoic acid receptors (RARs) are ligand‐dependent transcription factors that play critical roles in cell differentiation, embryonic development, and tumor suppression. RAR transcriptional activities are mediated by a growing family of nuclear receptor (NR) coregulators. Here we report the cloning and characterization of a novel protein RIF1 (receptor interacting factor) that interacts with RARα in vivo and in vitro. RIF1 encodes a novel 739 amino acid protein that is ubiquitously expressed in a variety of tissues and cell lines. GST‐pull down assays show that RIF1 also interacts with a number of other NRs. The interaction domain of RIF1 for RARα is located at the C‐terminal region of RIF1, between amino acids 512 and 674. RIF1 is localized exclusively in the cell nucleus and specifically to the nuclear matrix. Mutation of the nuclear localization signal abolishes this nuclear localization and causes RIF1 to appear in the cytoplasm. Co‐transfection of RIF1 with RAR causes RAR to localize to the nuclear matrix. RIF1 contains a strong transcriptional repression domain that robustly inhibits ligand‐dependent transcriptional activation by RARα. This domain is located to the distal C‐terminal 100 amino acids, distinct from the RARα‐interaction and nuclear matrix‐targeting domains. The transcriptional repression activity of RIF1 is mediated at least in part through direct recruitment of histone deacetylases. This study identifies RIF1 as a novel nuclear matrix transcription repressor, and suggests a potential role of RIF1 that regulates NR transcriptional activity. J. Cell. Biochem. 102: 1021–1035, 2007. © 2007 Wiley‐Liss, Inc.
A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene
Authors: Konoshita Tadashi , Fuchs Sébastien , Makino Yasukazu , Wakahara Shigeyuki , Miyamori Isamu 
Published: April 2007

AbstractThe regulation of renin gene expression is thought to be fundamental to regulation of the total renin–angiotensin system. The human renin gene contains a direct repeat (DR) motif AGGGGTCAC–AGGGCCA in the proximal region (−259/−245 bp), which contains similar sequence for nuclear receptor superfamily binding core motif, AGGTCA, and is the most similar to COUP‐TFII consensus. The DR motif was evaluated as a functional cis‐element with renal cortex and chorio‐decidual cells by footprint assay, electromobility shift assay (EMSA) and reporter assay. The DR motif site was protected by footprint analysis with a clear hypersensitive and a minor hypersensitive region in good accordance with the DR of the consensus. One of the binding proteins was strongly suspected to be COUP‐TFII‐consensus‐specific by EMSA. The DNA/protein complexes obtained with nuclear extract of renin producing cells could be completely blocked by homologous competitor and strongly blocked by the second‐half mutant oligonucleotide of the DR motif but not by the first‐half mutant oligonucleotide. Finally, the transcriptional activity of second‐half mutant construct is slightly elevated and that first‐half mutant construct is significantly stronger by twofold compared with wild type construct in reporter assay. These findings suggest that the DR motif site of the human renin gene functions as a negative regulatory element involved in a twofold repression of transcription and that member(s) of nucleic receptor superfamily bind the site and play important roles in the human renin gene expression with a possibility that one of the binding protein is COUP‐TFII. J. Cell. Biochem. 102: 1043–1050, 2007. © 2007 Wiley‐Liss, Inc.
Nuclear structure as a source of cancer specific biomarkers
Authors: Leman Eddy S. , Getzenberg Robert H. 
Published: April 2007

AbstractThere are few biomarkers that have been developed which have proven clinical utility for the detection and prognosis of cancer. Cancer is diagnosed today, in large part, by examining cells under the microscope and determining the shape and texture of the nucleus. The molecular underpinnings of this hallmark of cancer are the components of the nuclear matrix. Utilizing proteomics focused on this subset of proteins, biomarkers have been identified that are specific for cancer types including prostate, colon and bladder cancer. These cancer biomarkers now serve as the basis of assays which can specifically identify individuals with cancer by sampling their blood and/or urine. In addition, these may serve as potential therapeutic targeting or imaging approaches. J. Cell. Biochem. 104: 1988–1993, 2008. © 2007 Wiley‐Liss, Inc.
Validation of critical points in the electron density as descriptors by building quantitative structure–property relationships for the atomic polar tensor
Authors: Buttingsrud Bård , Alsberg Bjørn K. , Åstrand Per‐Olof 
Published: April 2007

AbstractA crucial component of research in the field of quantitative structure–activity/property relationships is the identification of molecular descriptors relevant to the activity or property of interest. Descriptors based on the topology of the electron density as formulated in Bader's theory of atoms in molecules are investigated in detail in this work. In a model study, the authors investigate their ability to predict the atomic polar tensor (the gradient of the molecular dipole moment), which contains information on the vibrational intensities in infrared spectroscopy and constitutes a scheme for partitioning the total charge distribution into atomic charges. The atomic polar tensor may therefore be used to investigate whether the descriptors give adequate information on the local electronic structure in the molecule. Both the trace of the atomic polar tensor and for planar molecules its out‐of‐plane component may be interpreted as definitions of atomic charges suitable for prediction. Hydrogen and carbon atoms in a set of 60 aromatic compounds with various substituents have been studied. Excellent results for prediction of hydrogen and carbon charges have been achieved with cross‐validated squared correlation coefficients between predicted and theoretical values varying from 0.92 and 0.977 for the most complex set of substituents when the value, Laplacian, and ellipticity of the electron density in the bond critical points are used as descriptors. The carbon charges defined from the trace of the atomic polar tensor are correlated with its out‐of‐plane component whereas such relationship is not observed for the hydrogen charges studied in this work. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Implementation of divide‐and‐conquer method including Hartree‐Fock exchange interaction
Authors: Akama Tomoko , Kobayashi Masato , Nakai Hiromi 
Published: April 2007

AbstractThe divide‐and‐conquer (DC) method, which is one of the linear‐scaling methods avoiding explicit diagonalization of the Fock matrix, has been applied mainly to pure density functional theory (DFT) or semiempirical molecular orbital calculations so far. The present study applies the DC method to such calculations including the Hartree‐Fock (HF) exchange terms as the HF and hybrid HF/DFT. Reliability of the DC‐HF and DC‐hybrid HF/DFT is found to be strongly dependent on the cut‐off radius, which defines the localization region in the DC formalism. This dependence on the cut‐off radius is assessed from various points of view: that is, total energy, energy components, local energies, and density of states. Additionally, to accelerate the self‐consistent field convergence in DC calculations, a new convergence technique is proposed. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Bicarbonate‐dependent pHi regulation by chondrocytes within the superficial zone of bovine articular cartilage
Authors: Simpkin Victoria L. , Murray Dianne H. , Hall Andrew P. , Hall Andrew C. 
Published: April 2007

AbstractControl of chondrocyte pH (pHi) determines articular cartilage matrix metabolism. However, the transporters of chondrocytes in situ throughout cartilage zones are unclear, and we tested the hypothesis that chondocytes within the superficial zone (SZ) utilise a HCO‐dependent system absent from other zones. Imaging of single BCECF‐labelled cells was used to monitor the pHi of in situ chondrocytes within the cartilage zones, and also that of cells isolated from the SZ or full depth (FD) explants. Resting pHi and intrinsic buffering power (βi) in HEPES‐buffered saline was not different between SZ and DZ cells, however the pHi of SZ chondrocytes was lower in HCO saline. Ammonium pre‐pulse was used to acid‐load cells and pHi recovery by in situ or isolated SZ chondrocytes shown to be totally dependent on HCO. pHi recovery rate was significantly (P < 0.05) greater for in situ cells, suggesting that isolation damaged the HCO‐dependent system. Recovery of pHi by in situ cells was blocked by the anion transport inhibitor DIDS, and partially inhibited by EIPA probably non‐specifically. Recovery of pHi by acidified MZ or DZ cells or those isolated from FD explants was not affected by HCO (P > 0.05). Na+‐dependent HCO‐(NBC) transporters were identified in SZ chondrocytes by fluorescence immunohistochemistry suggesting that this system might account for the HCO‐dependent recovery of pHi. Bovine articular cartilage chondrocytes possess a HCO‐dependent transporter which plays a key role in pHi regulation in cells in the SZ, but not in chondrocytes within deeper cartilage zones. J. Cell. Physiol. 212:600–609, 2007. © 2007 Wiley‐Liss, Inc.