bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex chromosome turnover plays an important role in the maintenance of barriers to post-speciation introgression in willows
Authors: Xue Zhi-Qing , Applequist Wendy L , Hörandl Elvira , He Li 
Published: March 2024

Abstract
Almost all species in the genus Salix (willow) are dioecious and willows have variable sex-determining systems, the role of this variation in maintaining species barriers is relatively untested. We first analyzed the sex determination systems (SDS) of two species, Salix cardiophylla and Salix interior, whose positions in the Salix phylogeny make them important for understanding a sex chromosome turnover that has been detected in their relatives, and that changed the system from male (XX/XY) to female (ZW/ZZ) heterogamety. We show that both species have male heterogamety, with sex-linked regions (SLRs) on chromosome 15 (termed a 15XY system). The SLRs occupy 21.3% and 22.8% of the entire reference chromosome, respectively. By constructing phylogenetic trees, we determined the phylogenetic positions of all the species with known SDSs. Reconstruction of ancestral SDS character states revealed that the 15XY system is likely the ancestral state in willows. Turnovers of 15XY to 15ZW and 15XY to 7XY likely contributed to early speciation in Salix and gave rise to major groups of the Vetrix and Salix clades. Finally, we tested introgression among species in the phylogenetic trees based on both autosomes and SLRs separately. Frequent introgression was observed among species with 15XY, 15ZW, and 7XY on autosomes, in contrast to the SLR datasets, which showed less introgression, and in particular no gene flow between 15ZW and 7XY species. We argue that, although SDS turnovers in willow speciation may not create complete reproductive barriers, the evolution of SLRs plays important roles in preventing introgression and maintaining species boundaries.
The landscape and predicted roles of structural variants in Fusarium graminearum genomes
Authors: Dhakal Upasana , Kim Hye-Seon , Toomajian Christopher 
Published: March 2024

Abstract
Structural rearrangements, such as inversions, translocations, duplications, and large insertions and deletions, are large-scale genomic variants that can play an important role in shaping phenotypic variation and in genome adaptation and evolution. We used chromosomal-level assemblies from eight Fusarium graminearum isolates to study structural variants and their role in fungal evolution. We generated the assemblies of four of these genomes after Oxford Nanopore sequencing. A total of 87 inversions, 159 translocations, 245 duplications, 58,489 insertions, and 34,102 deletions were detected. Regions of high recombination rate are associated with structural rearrangements, and a significant proportion of inversions, translocations, and duplications overlap with the repeat content of the genome, suggesting recombination and repeat elements are major factors in the origin of structural rearrangements in F. graminearum. Large insertions and deletions introduce presence–absence polymorphisms for many genes, including secondary metabolite biosynthesis cluster genes and predicted effectors genes. Translocation events were found to be shuffling predicted effector-rich regions of the genomes and are likely contributing to the gain and loss of effectors facilitated by recombination. Breakpoints of some structural rearrangements fall within coding sequences and are likely altering the protein products. Structural rearrangements in F. graminearum thus have an important role to play in shaping pathogen–host interactions and broader evolution through genome reorganization, the introduction of presence–absence polymorphisms, and changing protein products and gene regulation.
Systemic lupus erythematosus presenting with pneumoperitoneum without evidence of pneumatosis cystoides intestinalis—when not to operate—a case report
Authors: Iftekhar Wafa , Shaikh Hafsa , Alvi Abdul R 
Published: March 2024

Abstract
Pneumoperitoneum in patients with systemic lupus erythematosus is commonly recognized as a surgical emergency that requires exploration. However, it may not be associated with bowel perforation and may be a benign disease manifestation. We present a case of a young patient who developed spontaneous pneumoperitoneum after pulse steroid therapy for lupus enteritis and was successfully managed conservatively. Patients with connective tissue disease may present with pneumoperitoneum, with or without pneumatosis cystoides intestinalis. Therefore, a detailed clinical history, thorough clinical examination, and laboratory parameters should be evaluated before proceeding with surgical intervention. A conservative approach may be attempted in patients with spontaneous pneumoperitoneum, and surgery should only be considered if clinical deterioration occurs.
Multilevel posterior spinal fusion following percutaneous third generation kyphoplasty complicated by vertebral compression fracture progression
Authors: Jung Bongseok , Abbas Anas , Han Justin , Ngan Alex , Katz Austen , Essig David 
Published: March 2024

Abstract
Newer third generation percutaneous kyphoplasty (PKs) may minimize risks associated with older generation kyphoplasties such as new adjacent fractures, fracture progression, cement leakage, neurologic sequelae, and kyphosis. Additionally, posterior pedicle spinal fusion (PPSF) may minimize risk of long-term complications following PKs while maximizing the benefits of stable spinal alignment. The patient developed adjacent fracture progression, posterior retropulsion, and kyphosis following third generation kyphoplasty. Vertebral compression fracture progression was corrected and prolonged symptomatic relief was successfully achieved with T11-L4 PPSF and L1-L2 laminectomy. Postoperative follow-ups at 2, 4, 7 weeks, 1 and 2 years showed continued symptomatic improvement in back pain with resolution of thigh and groin pain. This case supports the use of PPSF in third generation PK-related complications to provide long-term symptom relief and improve quality of life in patients with severe osteoporotic compression fractures.
Does Racial Bias Explain the Black-White Sentencing Gap across U.S. Courts?
Authors: Light Michael T , Vachuska Karl 
Published: March 2024

Abstract
It is widely speculated that prejudicial attitudes and implicit biases are fundamental to understanding racial disparities in criminal punishment. Yet surprisingly little research links measures of racial bias to data on criminal court decision-making. This article fills this gap by combining multiple aggregate measures of implicit and explicit racial bias with data from U.S. federal courts to examine whether racial disparities in sentencing are associated with prejudicial attitudes within the surrounding court context. We find no evidence that racial biases, whether implicit or explicit, significantly influence racial sentencing disparities across U.S. district courts. Nor do we find evidence that racial biases yield greater sentencing disparities in supplementary analyses using county-level court data. We do, however, find suggestive evidence that the prosecutorial application of mandatory minimums is sensitive to the level of racial bias within a court’s jurisdiction. Specifically, we find that Black defendants are disproportionately charged in districts with greater explicit racial animus.
The effects of TiO2, ZnO, IONs and Al2O3 metallic nanoparticles on the CYP1A1 and NBN transcripts in rat liver
Authors: Moselhy Walaa A , Ibrahim Marwa A , Khalifa Ahlam G , El-Nahass El-Shaymaa , Hassan Nour El-Houda Y 
Published: March 2024

Abstract

Introduction
Metal oxide nanoparticles are currently used widely in many aspects of human and animal life with broad prospects for biomedical purposes. The present work was carried out to investigate the effects of orally administrated TiO2NPs, ZnONPs, IONs and Al2O3NPs on the mRNA expression level of CYP 1A1 and NBN in the rat liver.

Materials and Methods
Four groups of male Albino rats were given their respective treatment orally for 60 days in a dose of 1/20 of the LD50 TiO2NPs (600 mg/Kg b.wt/day), ZnONPs (340 mg/Kg b.wt/day), IONs (200 mg/kg b.wt/day) and Al2O3NPs (100 mg/Kg b.wt/day) and a fifth group served as a control group.

Rresults
The mRNA level of CYP 1A1 and NBN showed up-regulation in all the NPs-treated groups relative to the control group. ZnONPs group recorded the highest expression level while the TiO2NPs group showed the lowest expression level transcript. Conclusion:The toxic effects produced by these nanoparticles were more pronounced in the case of zinc oxide, followed by aluminum oxide, iron oxide nanoparticles and titanium dioxide, respectively.
The Plastic Surgery Central Application versus ERAS: Which is Preferred?
Authors: Sarac Benjamin A. , Jackson Kianna , Schwartz Rachel , Gosman Amanda A. , Lin Samuel J. , Janis Jeffrey E. 
Published: March 2024

Background:
The Plastic Surgery Central Application (PSCA), designed to provide an equitable and streamlined application for both applicants and programs, was first designed in 2019, piloted in the 2020–21 application cycle, and is now in its fourth cycle in 2023–24. It has included preference signaling since the 2022–23 cycle, a feature in which applicants can send five “signals” to programs to express interest. We surveyed both program directors (PDs) and applicants following the 2023 match on their perceptions of PSCA versus Electronic Residency Application Service (ERAS).

Methods:
Surveys were deployed to applicants from three integrated plastic surgery programs during the 2022–23 cycle and all PDs. Respondents were asked basic demographic information, which application system they preferred: PSCA or ERAS, how well they were able to highlight/evaluate different areas of the application, and about their experiences specifically with preference signaling.

Results:
Forty-two (48%) PDs and 93 (29%) applicants responded. Most PDs (72%) and applicants (59%) preferred PSCA, with only 18% and 27% preferring ERAS. The remainder had no preference. Ninety-three percent of applicants reported that the cost savings of the PSCA were important. Most applicants (78%) and PDs (80%) were in favor or strongly in favor of the preference signaling program.

Conclusions:
Most applicants and PDs prefer PSCA over ERAS. These data, in conjunction with the cost savings, suggest that the PSCA may be a better alternative for the integrated plastic surgery match. Future analyses of these application systems will help provide the best application for prospective residents.
ANP32E drives vulnerability to ATR inhibitors by inducing R-loops-dependent Transcription Replication Conflicts in Triple Negative Breast Cancer
Authors: Lago Sara , Poli Vittoria , Fol Lisa , Botteon Mattia , Fasciani Alessandra , Turdo Alice , Gaggianesi Miriam , Todaro Matilde , Ciani Yari , D’Amato Giacomo , Demichelis Francesca , Zippo Alessio 
Published: March 2024

AbstractOncogene-induced replicative stress (RS) plays a central role in tumor progression, leading to genomic instability by eliciting transcription replication conflicts (TRCs), which represent the major source of R-loops, that ultimately favors the onset of the DNA damage response (DDR). We investigated the pathogenic contribution of chromatin factors in increasing TRCs and R-loop frequencies in cancer. We found that in breast cancer patients the concomitant upregulation of MYC and the H2A.Z-specific chaperone ANP32E correlated with an increase genome instability. Genome-wide profiling revealed that the ANP32E-dependent increases turnover of H2A.Z altered RNApol II processivity, leading to accumulation of long R-loops at TRCs. We showed that ANP32E upregulation increases TRCs and activates an ATR-dependent DDR, which predispose cancer cells to R-loop-mediated genomic fragility. By exploiting the vulnerability of ANP32E-expressing cancer cells to ATR inhibitors, we found that tumors relied on this DDR pathway, whose inhibition halted their pro-metastatic capacity.
Investors’ intention toward green investment: an extension of the theory of planned behavior
Authors: Hemdan Waleed , Zhang Jian 
Published: March 2024

PurposeThis study investigates how to motivate behavioral intentions toward green investment (BIGI) with the moderating effect of social media platforms usage (SMPU) among individual investors in Egypt.Design/methodology/approachThe study used partial least squares structural equation modeling (PLS-SEM) to analyze the data and test hypotheses based on a sample of 550 individual investors with investment experience.FindingsThe results show that attitude, subjective norm (SN), and perceived behavioral control (PBC) have a significant relationship with investors' behavioral intention toward green investment. The moderating effect of (SMPU) supported the relationship between (SN), (PBC), and (BIGI), but (SMPU) does not support the relationship between attitude and (BIGI).Practical implicationsThis study provides some implications for investment providers, service providers, and policymakers.Originality/valueDespite the increasing global interest in climate change and its consequent opportunities and challenges for business, previous studies did not strongly emphasize green investment. So, based on the theory of planned behavior (TPB), this study sheds light on the motivational factors that may push investors' behavioral intentions toward green investment. With the increasing interest in digital transformation, the study also examined how digital platforms support (BIGI), especially in Egypt as a developing country.
An examination of existing research on academic libraries and the social web
Authors: Choi Namjoo 
Published: March 2024

PurposeThe purpose of this study is to examine research on academic libraries and the social web published from July 1, 2019, to June 30, 2023.Design/methodology/approachThe article search and filtering procedures mirrored those of Choi and Harper (2020) and Carlsson (2015), resulting in a total sample size of 93 articles. These articles underwent examination based on the same eight variables (i.e. journal outlet, research theme, publication year, social web type, method, keyword, study participant type and study country) as employed by Choi and Harper (2020) and Carlsson (2015), with the addition of two new variables (i.e. research purpose and the impact of COVID-19).FindingsThe research article volume has consistently maintained a stable trend. A notable difference from Choi and Harper (2020) and Carlsson (2015) is the rise of “user perspectives” as the second most prevalent theme. Unlike Choi and Harper (2020), the “survey” method is predominant. Many research purposes, excluding “marketing and promotion,” lack attention. Additionally, there’s a dearth of studies on the impact of COVID-19.Research limitations/implicationsThe findings from the study not only offer a snapshot of the current research landscape on academic libraries and their engagement with the social web but also offer insights for future scholarly endeavors.Originality/valueThere is a limited effort in exploring the recent literature regarding the role of the social web in academic libraries. This study serves as a valuable guide for contributing to this dynamic research stream and provides various up-to-date implications.
Evaluation of Trichopria anastrephae performance as parasitoid of Drosophila suzukii and Zaprionus indianus, under controlled laboratory conditions
Authors: Funes C. F. , Rendon D. , Saez J. V. , Allori Stazzonelli E. , Pastor L. C. , Gibilisco S. M. , Bouvet J. P. , Maza N. , Kirschbaum D. S. 
Published: March 2024

AbstractNative parasitoids are potential tools for the biological control of invasive exotic pests, such as the frugivorous flies spotted‐wing drosophila, Drosophila suzukii Matsumura, and African fig fly, Zaprionus indianus Gupta (both Diptera: Drosophilidae). Following biological invasions, the establishment of exotic species may be restricted, among other things, by their susceptibility to native or established natural enemies. The Neotropical‐native pupal endoparasitoid Trichopria anastrephae Costa Lima (Hymenoptera: Diapriidae), although primarily associated with hosts of the genus Anastrepha (Tephritidae), has also been described to attack Drosophilidae. There are few studies on the interaction between parasitoids native to Argentina and D. suzukii and/or Z. indianus. The present work evaluates the parasitism of T. anastrephae on both species of drosophilids under controlled conditions. Ten larvae (L3) of each host species were exposed separately to a couple of adult T. anastrephae for 72 h (n = 10). For both control and parasitoid treatment, 10 larvae (L3) of D. suzukii and Z. indianus were placed in 10 flasks (repetition) at the L3 stage. Host emergence, the infestation degree index (DI), and the parasitism success rate (SP) were calculated. Drosophila suzukii and Z. indianus emergence differed significantly in controlled conditions being 41% higher for Z. indianus than for D. suzukii. The DI was 100% in D. suzukii and 38% in Z. indianus, and the SP was 48% both in D. suzukii and in Z. indianus. These findings indicate that the native parasitoid T. anastrephae has a high potential as a biocontrol strategy against D. suzukii. In addition, the efficiency of T. anastrephae against Z. indianus, an invasive frugivorous widely spread in the Neotropical region, was evaluated for the first time.
Trends in observation stays for Medicare beneficiaries with and without Alzheimer's disease and related dementias
Authors: Burke Laura G. , Burke Ryan C. , Duggan Ciara E. , Figueroa Jose F. , Boltz Marie , Fick Donna M. , Orav E. John , Marcantonio Edward R. 
Published: March 2024

AbstractBackgroundThere has been a marked rise in the use of observation care for Medicare beneficiaries visiting the emergency department (ED) in recent years. Whether trends in observation use differ for people with Alzheimer's disease and Alzheimer's disease‐related dementias (AD/ADRD) is unknown.MethodsUsing a national 20% sample of Medicare beneficiaries ages 68+ from 2012 to 2018, we compared trends in ED visits and observation stays by AD/ADRD status for beneficiaries visiting the ED. We then examined the degree to which trends differed by nursing home (NH) residency status, assigning beneficiaries to four groups: AD/ADRD residing in NH (AD/ADRD+ NH+), AD/ADRD not residing in NH (AD/ADRD+ NH‐), no AD/ADRD residing in NH (AD/ADRD‐ NH+), and no AD/ADRD not residing in NH (AD/ADRD‐ NH‐).ResultsOf 7,489,780 unique beneficiaries, 18.6% had an AD/ADRD diagnosis. Beneficiaries with AD/ADRD had more than double the number of ED visits per 1000 in all years compared to those without AD/ADRD and saw a faster adjusted increase over time (+26.7 vs. +8.2 visits/year; p < 0.001 for interaction). The annual increase in the adjusted proportion of ED visits ending in observation was also greater among people with AD/ADRD (+0.78%/year, 95% CI 0.77–0.80%) compared to those without AD/ADRD (+0.63%/year, 95% CI 0.59–0.66%; p < 0.001 for interaction). Observation utilization was greatest for the AD/ADRD+ NH+ population and lowest for the AD/ADRD‐ NH‐ population, but the AD/ADRD+ NH‐ group saw the greatest increase in observation stays over time (+15.4 stays per 1000 people per year, 95% CI 15.0–15.7).ConclusionsMedicare beneficiaries with AD/ADRD have seen a disproportionate increase in observation utilization in recent years, driven by both an increase in ED visits and an increase in the proportion of ED visits ending in observation.
Midwife-led birthing centres in Bangladesh, Pakistan and Uganda: an economic evaluation of case study sites
Authors: Callander Emily J , Scarf Vanessa , Nove Andrea , Homer Caroline , Carrandi Alayna , Abdullah Abu Sayeed , Clow Sheila , Halim Abdul , Mbalinda Scovia Nalugo , Nabirye Rose Chalo , Rahman AKM Fazlur , Rasheed Saad Ibrahim , Turk Arslan Munir , Bazirete Oliva , Turkmani Sabera , Forrester Mandy , Mandke Shree , Pairman Sally , Boyce Martin 
Published: March 2024

IntroductionAchieving the Sustainable Development Goals to reduce maternal and neonatal mortality rates will require the expansion and strengthening of quality maternal health services. Midwife-led birth centres (MLBCs) are an alternative to hospital-based care for low-risk pregnancies where the lead professional at the time of birth is a trained midwife. These have been used in many countries to improve birth outcomes.MethodsThe cost analysis used primary data collection from four MLBCs in Bangladesh, Pakistan and Uganda (n=12 MLBC sites). Modelled cost-effectiveness analysis was conducted to compare the incremental cost-effectiveness ratio (ICER), measured as incremental cost per disability-adjusted life-year (DALY) averted, of MLBCs to standard care in each country. Results were presented in 2022 US dollars.ResultsCost per birth in MLBCs varied greatly within and between countries, from US$21 per birth at site 3, Bangladesh to US$2374 at site 2, Uganda. Midwife salary and facility operation costs were the primary drivers of costs in most MLBCs. Six of the 12 MLBCs produced better health outcomes at a lower cost (dominated) compared with standard care; and three produced better health outcomes at a higher cost compared with standard care, with ICERs ranging from US$571/DALY averted to US$55 942/DALY averted.ConclusionMLBCs appear to be able to produce better health outcomes at lower cost or be highly cost-effective compared with standard care. Costs do vary across sites and settings, and so further exploration of costs and cost-effectiveness as a part of implementation and establishment activities should be a priority.
How valid is a prescription-based multimorbidity index (Rx-risk) in predicting mortality in the Outcomes and Multimorbidity In Type 2 diabetes (OMIT) study? A nation-wide registry-based cohort study from Norway
Authors: Igland Jannicke , Forster Rachel , Jenum Anne Karen , Strandberg Ragnhild B , Berg Tore Julsrud , Røssberg Jan Ivar , Iversen Marjolein Memelink , Buhl Esben Selmer 
Published: March 2024

ObjectiveThe prescription-based Rx-risk index has previously been developed to measure multimorbidity. We aimed to adapt and evaluate the validity of the Rx-risk index in prediction of mortality among persons with type 2 diabetes.DesignRegistry-based study.SettingAdults with type 2 diabetes in Norway identified within the ‘Outcomes and Multimorbidity In Type 2 diabetes’ cohort, with linkage to prescriptions from the Norwegian Prescription Database and mortality from the Population Registry.ParticipantsWe defined a calibration sample of 42 290 adults diagnosed with type 2 diabetes 1950–2013, and a temporal validation sample of 7085 adults diagnosed 2014–2016 to evaluate the index validity over timePrimary outcome measureAll-cause mortalityMethodsFor the calibration sample, dispensed drug prescriptions in 2013 were used to define 44 morbidity categories. Weights were estimated using regression coefficients from a Cox regression model with 5 year mortality as the outcome and all morbidity categories, age and sex included as covariates. The Rx-risk index was computed as a weighted sum of morbidities. The validity of the index was evaluated using C-statistic and calibration plots.ResultsIn the calibration sample, mean (SD) age at start of follow-up and duration of diabetes was 63.8 (12.4) and 10.1 (7.0) years, respectively. The overall C-statistic was 0.82 and varied from 0.74 to 0.85 when stratifying on age groups, sex, level of education and country of origin. In the validation sample, mean (SD) age and duration of diabetes was 59.7 (13.0) and 2.0 (0.8) years, respectively. Despite younger age, shorter duration of diabetes and later time period, the C-index was high both in the total sample (0.84) and separately for men (0.83) and women (0.84).ConclusionsThe Rx-risk index showed good discrimination and calibration in predicting mortality and thus presents a valid tool to assess multimorbidity among persons with type 2 diabetes.