bluuebunny/crossref_metadata_2025_split huggingface dataset:
The role of the IDDM2 locus in the susceptibility of UK APS1 subjects to type 1 diabetes mellitus
Authors: Adamson K. A. , Cheetham T. D. , Kendall‐Taylor P. , Seckl J. R. , Pearce S. H. S. 
Published: February 2007

SummaryAutoimmune polyendocrinopathy syndrome type 1 (APS1) is characterized by autoimmune destruction of endocrine tissues and chronic mucocutaneous candidiasis. Type 1 diabetes (T1D) affects 12–25% of patients with APS1, and the prediction of whether this complication will affect an individual is not currently possible. However, alleles of a variable number tandem repeat (VNTR) 5′ of the insulin gene are known to influence the development of T1D in the general, non‐APS1 population. Therefore, we investigated the prevalence of these IDDM2 alleles in British Caucasian patients with APS1. The study employed genotyping of 33 patients with APS1 for the HphI polymorphism that is in tight linkage disequilibrium with the insulin gene VNTR alleles. Thirty‐three patients with APS1 were studied, the mean age was 23.5 years and 24% have T1D. Six of eight (75%) APS1 patients with T1D were homozygous for the class I INS VNTR (susceptibility) allele, compared with eight of 25 (32%) of APS1 patients without T1D (P = 0.042). Our data suggest an association between the development of T1D and homozygosity for the T1D susceptibility class IINS VNTR allele in patients with APS1.
Expression of the miR-17-92 polycistron in chronic myeloid leukemia (CML) CD34+ cells
Authors: Venturini Letizia , Battmer Karin , Castoldi Mirco , Schultheis Beate , Hochhaus Andreas , Muckenthaler Martina U. , Ganser Arnold , Eder Matthias , Scherr Michaela 
Published: February 2007

Abstract
Aberrant micro RNA (miRNA) expression has been described in human malignancies including B-cell lymphomas. We here report BCR-ABL– and c-MYC–dependent regulation of miRNA expression in chronic myeloid leukemia (CML) using microarray analysis (miCHIP) and miRNA-specific quantitative real-time reverse transcriptase–polymerase chain reaction (miR-qRT-PCR). In 3 bcr-abl–positive cell lines, expression of miRNAs encoded within the polycistronic miR-17-92 cluster is specifically down-regulated (2- to 5-fold) by both imatinib treatment and anti–BCR-ABL RNA interference (RNAi). In addition, anti–c-MYC RNAi reduces miR-17-92 expression in K562 cells in which miRNAs can specifically repress reporter gene expression, as demonstrated by specific miRNA inhibition with antagomirs. Furthermore, lentivirus-mediated overexpression of polycistronic miRNAs in K562 cells confers increased proliferation, partial resistance against anti–c-MYC RNAi, and enhanced sensitivity to imatinib-induced cell death. Finally, we determined miR-17-92 expression in purified normal (n = 4), early chronic-phase (CP) (n = 24), and blast-crisis (BC) (n = 7) CML CD34+ cells and found up-regulation of polycistronic pri-miRNA transcripts in CML and mature miRNAs in CP but not in BC CML. These data are in accordance with a BCR-ABL–c-MYC–miR-17-92 pathway that mediates enhanced miRNA expression in CP but not BC CML CD34+ cells. Altered miRNA expression may contribute to the pathophysiology of the disease and may provide potential targets for therapeutic intervention.
DC-HIL is a negative regulator of T lymphocyte activation
Authors: Chung Jin-Sung , Sato Kota , Dougherty Irene I. , Cruz Ponciano D. , Ariizumi Kiyoshi 
Published: February 2007

Abstract
T-cell activation is the net product of competing positive and negative signals transduced by regulatory molecules on antigen-presenting cells (APCs) binding to corresponding ligands on T cells. Having previously identified DC-HIL as a receptor expressed by APCs that contains an extracellular immunoglobulin (Ig)–like domain, we postulated that it plays a role in T-cell activation. To probe this function, we created soluble recombinant DC-HIL, which we observed to bind activated (but not resting) T cells, indicating that expression of the putative ligand on T cells is induced by activation. Binding of DC-HIL to naive T cells attenuated these cells' primary response to anti-CD3 antibody, curtailing IL-2 production, and preventing entry into the cell cycle. DC-HIL also inhibited reactivation of T cells previously activated by APCs (secondary response). By contrast, addition of soluble DC-HIL to either allogeneic or ovalbumin-specific lymphocyte reactions augmented T-cell proliferation, and its injection into mice during the elicitation (but not sensitization) phase of contact hypersensitivity exacerbated ear-swelling responses. Mutant analyses showed the inhibitory function of DC-HIL to reside in its extracellular Ig-like domain. We conclude that endogenous DC-HIL is a negative regulator of T lymphocyte activation, and that this native inhibitory function can be blocked by exogenous DC-HIL, leading to enhanced immune responses.
Rev-dependent lentiviral expression vector
Authors: Wu Yuntao , Beddall Margaret H , Marsh Jon W 
Published: February 2007

AbstractBackgroundHIV-responsive expression vectors are all based on the HIV promoter, the long terminal repeat (LTR). While responsive to an early HIV protein, Tat, the LTR is also responsive to cellular activation states and to the local chromatin activity where the integration has occurred. This can result in high HIV-independent activity, and has restricted the use of LTR-based reporter vectors to cloned cells, where aberrantly high expressing (HIV-negative) cells can be eliminated. Enhancements in specificity would increase opportunities for expression vector use in detection of HIV as well as in experimental gene expression in HIV-infected cells.ResultsWe have constructed an expression vector that possesses, in addition to the Tat-responsive LTR, numerous HIV DNA sequences that include the Rev-response element and HIV splicing sites that are efficiently used in human cells. It also contains a reading frame that is removed by cellular splicing activity in the absence of HIV Rev. The vector was incorporated into a lentiviral reporter virus, permitting detection of replicating HIV in living cell populations. The activity of the vector was measured by expression of green fluorescence protein (GFP) reporter and by PCR of reporter transcript following HIV infection. The vector displayed full HIV dependency.ConclusionAs with the earlier developed Tat-dependent expression vectors, the Rev system described here is an exploitation of an evolved HIV process. The inclusion of Rev-dependency renders the LTR-based expression vector highly dependent on the presence of replicating HIV. The application of this vector as reported here, an HIV-dependent reporter virus, offers a novel alternative approach to existing methods,in situPCR or HIV antigen staining, to identify HIV-positive cells. The vector permits examination of living cells, can express any gene for basic or clinical experimentation, and as a pseudo-typed lentivirus has access to most cell types and tissues.
(S)-Armepavine inhibits human peripheral blood mononuclear cell activation by regulating Itk and PLCγ activation in a PI-3K-dependent manner
Authors: Liu Chih-Peng , Kuo Yuh-Chi , Shen Chien-Chang , Wu Ming-Hsi , Liao Jyh-Fei , Lin Yun-Lian , Chen Chieh-Fu , Tsai Wei-Jern 
Published: February 2007

AbstractChinese herbs are useful edible and medicinal plants for their immune modulatory functions. We have proven that (S)-armepavine (C19H23O3N; MW313) from Nelumbo nucifera inhibits the proliferation of human PBMCs activated with PHA and improves autoimmune diseases in MRL/MpJ-lpr/lpr mice. In the present study, the pharmacological activities of (S)-armepavine were evaluated in PHA-activated PBMCs. The results showed that (S)-armepavine suppressed PHA-induced PBMC proliferation and genes expression of IL-2 and IFN-γ without direct cytotoxicity. Inhibition of NF-AT and NF-κB activation suggested phospholipase Cγ (PLCγ)-mediated Ca2+ mobilization and protein kinase C activation were blocked by (S)-armepavine. Phosphorylation of PLCγ is regulated by lymphocyte-specific kinase (Lck), ZAP-70, and IL-2-inducible T cell kinase (Itk). We found (S)-armepavine inhibited PHA-induced phosphorylation of Itk and PLCγ efficiently but did not influence Lck or ZAP-70 phosphorylation. In addition, ZAP-70-mediated pathways, such as the association of linker for activation of T cells with PLCγ and activation of ERK, were also intact in the presence of (S)-armepavine. Finally, reduction of phosphoinositide 3,4,5-trisphosphate formation and Akt phosphorylation suggested that (S)-armepavine inhibited Itk, and PLCγ phosphorylation might be a result of the influence of PI-3K activation. Addition of exogenous IL-2 or PMA/A23187 rescued PBMC proliferation in the presence of (S)-armepavine. Therefore, we concluded that (S)-armepavine inhibited PHA-induced cell proliferation and cytokine production in a major way by blocking membrane-proximal effectors such as Itk and PLCγ in a PI-3K-dependent manner.
Altered Skeletal Muscle Fiber Composition and Size Precede Whole-Body Insulin Resistance in Young Men with Low Birth Weight
Authors: Jensen Christine B. , Storgaard Heidi , Madsbad Sten , Richter Erik A. , Vaag Allan A. 
Published: February 2007

Abstract
Context: Low birth weight (LBW), a surrogate marker of an adverse fetal milieu, is linked to muscle insulin resistance, impaired insulin-stimulated glycolysis, and future risk of type 2 diabetes. Skeletal muscle mass, fiber composition, and capillary density are important determinants of muscle function and metabolism, and alterations have been implicated in the pathogenesis of insulin resistance.
Objective: The aim of this study was to investigate whether an adverse fetal environment (LBW) induces permanent changes in skeletal muscle morphology, which may contribute to the dysmetabolic phenotype associated with LBW.
Design and Subjects: Vastus lateralis muscle was obtained by percutaneous biopsy from 20 healthy 19-yr-old men with birth weights at 10th percentile or lower for gestational age (LBW) and 20 normal birth weight controls, matched for body fat, physical fitness, and whole-body glucose disposal. Myofibrillar ATPase staining was used to classify muscle fibers as type I, IIa, and IIx (formerly type IIb), and double immunostaining was performed to stain capillaries (LBW, n = 8; normal birth weight, n = 12).
Results: LBW was associated with increased proportion of type IIx fibers (+66%; P = 0.03), at the expense of decreased type IIa fibers (−22%; P = 0.003). No significant change was observed in proportion of type I fibers (+16%; P = 0.11). In addition, mean area of type IIa fibers was increased (+29%; P = 0.01) and tended to be increased for type I fibers as well (+17%; P = 0.08). Capillary density was not significantly different between groups.
Conclusion: Alterations in fiber composition and size may contribute to development of type 2 diabetes in individuals with LBW.
Moxifloxacin and Torsade de Pointes
Authors: Dale Krista M , Lertsburapa Kirkeith , Kluger Jeffrey , White C Michael 
Published: February 2007

Objective: To report a case of torsade de pointes in a patient receiving moxifloxacin. Case Summary: An 87-year-old woman was admitted to the hospital for pneumonia, and antibiotic therapy with intravenous moxifloxacin 400 mg/day was initiated. The patient was noted to have significant QTc interval prolongation 2 hours after administration of moxifloxacin and developed torsade de pointes 8–10 hours after moxifloxacin administration. She was converted back to normal sinus rhythm after a precordial thump. Moxifloxacin was discontinued, and the woman's QTc interval subsequently returned to baseline. Discussion: Torsade de pointes is a life-threatening arrhythmia that has previously been associated with the use of fluoroquinolones. Minimal information is available regarding the risk of torsade de pointes with moxifloxacin. According to the Naranjo probability scale, the episode in this case was probably related to administration of intravenous moxifloxacin. Conclusions: In patients with underlying risk factors for a prolonged QT interval, the use of moxifloxacin can lengthen the interval further and ultimately trigger episodes of torsade de pointes. Moxifloxacin administration in these patients therefore should be administered and monitored judiciously.
Formation of hard hematite-cemented solids in steam generators: An analog of lithification of Fe-containing sedimentary rocks
Authors: Labuda Ewa , Cherepakhov Galina , Barkatt Aaron 
Published: February 2007

AbstractThe formation of hard hematite in steam generators with relatively high levels (5–10 µg/L) of dissolved oxygen at temperatures around 280–290°C and pressures around 6–8 MPa can serve as an analog for the formation of hard hematite in sedimentary processes. Furthermore, in steam generators, as well as in nature, hematite is an effective cementing agent, capable of incorporating as much as twice its own weight of other solids to form a hard composite material. Laboratory simulations showed ferrihydrite to be the likely starting material for the formation of hard, dense hematite at temperatures much lower than those required for sintering of anhydrous hematite. These laboratory simulations, performed at temperatures around 260°C and pressures of ∼500 MPa, resulted in the formation of hard hematite or hematite-based composite solids over periods of 3–5 h, compared with several months in steam generators and many years in nature. The amount of water present during the synthesis (10–15% of the weight of dry ferrihydrite) and the gradual removal of water proved to be key parameters in the formation of hard, dense hematite. The mechanism, studied by means of X-ray diffractometry, Mössbauer spectroscopy and infrared spectroscopy, appeared to involve build-up, then gradual condensation of OH bridges, leading to the conversion of ferrihydrite to hydrohematite with approximately 4–5% of residual water. The presence of other solids, such as copper and its oxides, alumina and silica, in large quantities, resulted in smaller grain size of the hydrohematite product but did not affect its mechanical properties. On the other hand, the use of hydrazine to provide a reducing environment produced goethite during the precursor synthesis stage and soft magnetite during the pressing stage. However, whenever hematite was produced, it could not be subsequently reduced to magnetite by hydrazine under the reaction conditions specified above. The mechanical properties as well as the spectroscopic characteristics of the product of pressing agreed with observations on sedimentary hematite-cemented rocks.
Dimensions of women’s autonomy and the influence on maternal health care utilization in a North Indian city
Authors: Bloom Shelah S. , Wypij David , Das Gupta Monica 
Published: February 2007

Abstract
The dimensions of women’s autonomy and their relationship to maternal health care utilization were investigated in a probability sample of 300 women in Varanasi, India. We examined the determinants of women’s autonomy in three areas: control over finances, decision-making power, and freedom of movement. After we control for age, education, household structure, and other factors, women with closer ties to natal kin were more likely to have greater autonomy in each of these three areas. Further analyses demonstrated that women with greater freedom of movement obtained higher levels of antenatal care and were more likely to use safe delivery care. The influence of women’s autonomy on the use of health care appears to be as important as other known determinants such as education.
A sensitivity analysis of the bongaarts-feeney method for adjusting bias in observed period total fertility rates
Authors: Yi Zeng , Land Kenneth C. 
Published: February 2007

Abstract
Our sensitivity analysis shows that the adjusted TFR(t) using the formula of Bongaarts and Feeney (1998), which assumes an invariant shape for the fertility schedule, usually does not differ significantly from an adjusted TFR(t) that allows the shape of the fertility schedule to change at a constant annual rate. Because annual changes in the shape of the fertility schedules often are approximately constant except in abnormal conditions, the Bongaarts-Feeney (B-F) method is generally robust for producing reasonable estimates of the adjusted TFR(t). The adjusted TFR(t) neither represents any real cohort experiences from the past nor forecasts any future trend. It merely provides an improved reading of the period fertility measure, which reduces the tempo distortion.
Increasing longevity and medicare expenditures
Authors: Miller Tim 
Published: February 2007

Abstract
Official Medicare projections forecast that the elderly population will be less healthy and more costly over the next century. This prediction stems from the use of age as an indicator of health status: increases in longevity are assumed to increase demand for health care as individuals survive to older and higher-use ages. In this paper I suggest an alternative approach, in which time until death replaces age as the demographic indicator of health status. Increases in longevity are assumed to postpone the higher Medicare use and costs associated with the final decade of life. I contrast the two approaches, using mortality forecasts consistent with recent projections from the U.S. Census Bureau and the Social Security Administration. The time-until-death method yields significantly lower-cost forecasts. The hypothetical cost savings from improved health are small, however, relative to the size of the Medicare solvency problem caused by population aging.
Educational stratification by ethnicity in China: Enrollment and attainment in the early reform years
Authors: Hannum Emily 
Published: February 2007

Abstract
Using evidence about educational disparities, this article demonstrates the need for attention to minority populations in studies of social stratification in China. Analyses of data from a 1992 survey of children demonstrate substantial ethnic differences in enrollment among rural 7- to 14 year olds, with rates for ethnic Chinese boys roughly double those for girls from certain ethnic groups. Multivariate analyses indicate that the ethnic gap can be attributed, in part, to compositional differences in geographic location of residence and socioeconomic background. There is no general tendency of a greater gender gap for minorities than for the ethnic Chinese, but significant differences in the gender gap emerge across individual ethnic groups. Together with evidence from census data showing that ethnic disparities in junior high school transitions increased between 1982 and 1990, these results stress the continuing significance of ethnicity as a fundamental factor that conditions status attainment opportunities in China.
The effects of kin on child mortality in rural gambia
Authors: Sear Rebecca , Steele Fiona , McGregor Ian A. , Mace Ruth 
Published: February 2007

AbstractWe analyzed data that were collected continuously between 1950 and 1974 from a rural area of the Gambia to determine the effects of kin on child mortality. Multilevel event-history models were used to demonstrate that having a living mother, maternal grandmother, or elder sisters had a significant positive effect on the survival probabilities of children, whereas having a living father, paternal grandmother, grandfather, or elder brothers had no effect. The mother’s remarriage to a new husband had a detrimental effect on child survival, but there was little difference in the mortality rates of children who were born to monogamous or polygynous fathers. The implications of these results for understanding the evolution of human life-history are discussed.
Aids and the elderly of thailand: projecting familial impacts
Authors: Wachter Kenneth W. , Knodel John E. , Vanlandingham Mark 
Published: February 2007

Abstract
We apply aggregate demographic analysis and computer microsimulation to project the number of older Thais who will lose children to AIDS during their own lifetimes and to assess their involvement with ill children through caregiving and coresidence. Parental bereavements from AIDS are predicted to peak at around 80,000 per year between 2003 and 2007. Despite an HIV prevalence of only 2%, 13% of Thais who were over age 50 as of 1995 are likely to experience the loss of at least one adult child to AIDS, and 12% of them will lose multiple children. The chance of losing an adult child during onés lifetime will be 70% higher than if there were no AIDS epidemic.
The effects of early maternal employment on child cognitive development
Authors: Waldfogel Jane , Han Wen-Jui , Brooks-Gunn Jeanne 
Published: February 2007

Abstract
We investigated the effects of early maternal employment on children’s cognitive outcomes, using data from the National Longitudinal Survey of Youth on 1,872 children who can be followed from birth to age 7 or 8. We found some persistent adverse effects of first-year maternal employment and some positive effects of second- and third-year maternal employment on cognitive outcomes for non-Hispanic white children, but not for African American or Hispanic children. These effects are present even after we controlled for a range of individual and family characteristics that affect child development, including those that are likely to be correlated with maternal employment, such as breast-feeding and the use of nonmaternal child care. Controlling for family fixed effects reduces the effects of early maternal employment on some cognitive outcomes but not on others.
Cohort and life-course patterns in the relationship between education and health: A hierarchical approach
Authors: Lynch Scott M. 
Published: February 2007

AbstractRecent medical sociological research has examined whether the relationship between education and health is dynamic across age, whereas recent demographic research has examined whether the relationship varies across cohorts. In this study, I examine how cohort structures the influence of education on life-course health trajectories. At the cohort level, changes in education and in the distribution of health and mortality make cohort differences in education’s effect probable. At the life-course level, the effect of education may vary across age because the mediators of the education-health relationship may vary in their relevance to health across the life course. Using basic regression analyses and random-effects models of two national data sets, I find that the effect of education strengthens across age, that this pattern is becoming stronger across cohorts, and that these patterns are suppressed when either effect is ignored.
Creating the National War Labor Board: Franklin Roosevelt and the Politics of State Building in the Early 1940s
Authors: Workman Andrew A. 
Published: February 2007

In January 1942, President Franklin D. Roosevelt issued an executive order creating a National War Labor Board (NWLB) to arbitrate wartime industrial disputes. Roosevelt's order provided enormous power for the board, which could, on its own motion, intervene in any labor conflict it deemed a threat to “the effective prosecution of the war” and subsequently impose settlements on the parties. In practice, the board replaced free collective bargaining for the duration of the war. Most scholars of the era agree that the NWLB, operating at a time when New Deal labor policy was still in formation and many unions had not yet become entrenched in their industries, had a profound impact on the evolution of the American industrial relations system during the war and thereafter.Despite a superficial similarity to earlier labor boards, the NWLB was a curious creature born, in the words of one of its members, “out of deadlock,” and of a breed uncommon on the American political landscape. The board's authority was nominally grounded on an agreement by a 1941 national labor-management conference to eschew strikes and lockouts in lieu of arbitration. Yet this conference had reached an impasse and its “agreement” had been forced on the business delegates by Roosevelt.