bluuebunny/crossref_metadata_2025_split huggingface dataset:
Root resorptions induced by genetic disorders: A systematic review
Authors: Dupre Nicolas , Riou Margot C. , Isaac Juliane , Ferre François , Cormier‐Daire Valérie , Kerner Stéphane , de La Dure‐Molla Muriel , Nowwarote Nunthawan , Acevedo Ana Carolina , Fournier Benjamin P. J. 
Published: April 2024

AbstractObjectivesRoot resorption in permanent teeth is a common pathological process that often follows dental trauma or orthodontic treatment. More rarely, root resorption is a feature of genetic disorders and can help with diagnosis. Thus, the present review aims to determine which genetic disorders could induce pathological root resorptions and thus which mutated genes could be associated with them.MethodsWe conducted a systematic review following the PRISMA guidelines. Articles describing root resorptions in patients with genetic disorders were included from PubMed, Embase, Web of Science, and Google Scholar. We synthesized the genetic disorder, the type, severity, and extent of the resorptions, as well as the other systemic and oral symptoms and histological features.ResultsThe synthetic analysis included 25 studies among 937 identified records. We analyzed 21 case reports, three case series, and one cohort study. Overall, we highlighted 14 different pathologies with described root resorptions. Depending on the pathology, the sites of resorption, their extent, and their severity showed differences.ConclusionWith 14 genetic pathologies suspected to induce root resorptions, our findings are significant and enrich a previous classification. Among them, three metabolic disorders, three calcium–phosphorus metabolism disorders, and osteolysis disorders were identified.
Effect of pre‐emptive rituximab on EBV DNA levels and prevention of post‐transplant lymphoproliferative disorder in pediatric kidney transplant recipients: A case series from the pediatric nephrology research consortium
Authors: Ashoor Isa F. , Al‐Akash Samhar , Kizilbash Sarah , Moudgil Asha , Puliyanda Dechu , Ranabothu Saritha , Shi Yi , Dharnidharka Vikas 
Published: April 2024

AbstractBackgroundThere are scant data on the effect of rituximab on EBV DNA levels and prevention of post‐transplant lymphoproliferative disorder (PTLD) in pediatric kidney transplant recipients with EBV DNAemia.MethodsKidney transplant recipients with EBV DNAemia treated with rituximab to prevent PTLD between 7/1999 and 7/2019 at five pediatric centers were included. Those with confirmed PTLD at the onset of rituximab were excluded. Primary outcomes included percentage change in EBV DNAemia and occurrence of PTLD post rituximab.ResultsTwenty‐six pediatric kidney transplant recipients were included. Median age at transplant was 4 years (IQR 2.1–10.3). EBV DNA load monitoring by qPCR was performed at 1–3 month intervals. EBV DNAemia onset occurred at a median of 73 days post‐transplant (IQR 52–307), followed by DNAemia peak at a median of 268 days (IQR 112–536). Rituximab was administered at a median of 9 days post peak (IQR 0–118). Rituximab regimens varied; median dose 375 mg/m2 (IQR 375–439) weekly for 1–4 doses per course. Following rituximab, EBV DNA load decreased to <10% of baseline at 120 days in 20/26 patients; however, only 30% achieved complete resolution at last follow‐up (median 2094 days post‐transplant [IQR 1538–3463]). Two (7%) developed PTLD at 915 and 1713 days post rituximab. All recipients had functioning grafts. One death occurred in a child with PTLD following remission due to unrelated reasons.ConclusionsIn the largest pediatric kidney transplant recipient case series with EBV DNAemia given rituximab to prevent PTLD, rituximab achieved a short‐term reduction in DNA load; however, recurrent DNAemia is common.
(Non)governing machine: Seized activism and NGOization as forms of governance in Indonesia
Authors: Rasyadian Yuda 
Published: April 2024

AbstractNGOization is argued as a key process to the expansion of neoliberalized development, feminist movement, and authentic democratization. Anthropologists have challenged neoliberal assumptions behind NGOization. This article extends the debate by investigating how a nongovernmental organization (NGO) project that initially claimed to buttress egalitarian grassroots activism has undergone NGOization with its public discourse and practice integrated into state work, encapsulating what I theorize as “(non)governing machine”—the apparent nongovernment entity that possesses a systematic power and authority to regulate knowledge and population. Drawing from an ethnographic study of an NGO and local politics in Mollo, Indonesia, my argument is threefold. First, I argue that NGOization enables a specific form of governmentality that has allowed the state to increase its surveillance of, and power over, the population. Second, this article proposes that local politics can be a strong impetus to the establishment of NGOization. Finally, by coining the (non)governing machine, I theorize NGOization as a form of governance, pointing at the productivity and vulnerability of NGO form as a “hybrid formation” that grapples between local politics and transnational networks of power and capital.
Circadian rhythm and redox homeostasis candidate genes showed association with shallow elevation in Norway spruce
Authors: Caré O. , Chano V. , Erley M. , Rogge M. , Gailing O. 
Published: April 2024

Abstract

The analysis of genetic variation underlying local adaptation in natural populations, together with the response to different external stimuli, is currently a hot topic in forest sciences, with the aim of identifying genetic markers controlling key phenotypic traits of interest for their inclusion in restoration and breeding programs.

In Europe, one of the main tree species is Norway spruce (Picea abies (L.) H.Karst.). Using the MassARRAY® platform, 568 trees from North Rhine‐Westphalia (Germany) were genotyped with 94 single nucleotide polymorphisms (SNPs) related to circadian and growth rhythms, and to stress response. The association analysis of the selected markers with health status and elevation was performed using three different methods, and those identified by at least two of these were considered as high confidence associated SNPs.

While just five markers showed a weak association with health condition, 32 SNPs were correlated with elevation, six of which were considered as high confidence associated SNPs, as indicated by at least two different association methods. Among these genes, thioredoxin and pseudo response regulator 1 (PRR1) are involved in redox homeostasis and ROS detoxification, APETALA2‐like 3 (AP2L3), a transcription factor, is involved in seasonal apical growth, and a RPS2‐like is a disease resistance gene.

The function of some of these genes in controlling light‐dependent reactions and metabolic processes suggests signatures of adaptation to local photoperiod and the synchronization of the circadian rhythm. This work provides new insights into the genetic basis of local adaptation over a shallow elevation gradient in Norway spruce.
Photoprotective mechanisms in Elysia species hosting Acetabularia chloroplasts shed light on host‐donor compatibility in photosynthetic sea slugs
Authors: Morelli Luca , Havurinne Vesa , Madeira Diana , Martins Patrícia , Cartaxana Paulo , Cruz Sónia 
Published: April 2024

AbstractSacoglossa sea slugs have garnered attention due to their ability to retain intracellular functional chloroplasts from algae, while degrading other algal cell components. While protective mechanisms that limit oxidative damage under excessive light are well documented in plants and algae, the photoprotective strategies employed by these photosynthetic sea slugs remain unresolved. Species within the genus Elysia are known to retain chloroplasts from various algal sources, but the extent to which the metabolic processes from the donor algae can be sustained by the sea slugs is unclear. By comparing responses to high‐light conditions through kinetic analyses, molecular techniques, and biochemical assays, this study shows significant differences between two photosynthetic Elysia species with chloroplasts derived from the green alga Acetabularia acetabulum. Notably, Elysia timida displayed remarkable tolerance to high‐light stress and sophisticated photoprotective mechanisms such as an active xanthophyll cycle, efficient D1 protein recycling, accumulation of heat‐shock proteins and α‐tocopherol. In contrast, Elysia crispata exhibited absence or limitations in these photoprotective strategies. Our findings emphasize the intricate relationship between the host animal and the stolen chloroplasts, highlighting different capacities to protect the photosynthetic organelle from oxidative damage.
Untargeted Metabolomic Analysis of Leaves and Roots of Jatropha curcas Genotypes with Contrasting Levels of Phorbol Esters
Authors: Neto Domingos F. M. , Garrett Rafael , Domont Gilberto B. , Campos Francisco A. P. , Nogueira Fábio C. S. 
Published: April 2024

AbstractAimsPhorbol esters (PE) are toxic diterpenoids accumulated in physic nut (Jatropha curcas L.) seed tissues. Their biosynthetic pathway remains unknown, and the participation of roots in this process may be possible. Thus, we set out to study the deposition pattern of PE and other terpenoids in roots and leaves of genotypes with detected (DPE) and not detected (NPE) phorbol esters based on previous studies.Outline of data resourcesWe analyzed physic nut leaf and root organic extracts using LC‐HRMS. By an untargeted metabolomics approach, it was possible to annotate 496 and 146 metabolites in the positive and negative electrospray ionization modes, respectively.Key resultsPE were detected only in samples of the DPE genotype. Remarkably, PE were found in both leaves and roots, making this study the first report of PE in J. curcas roots. Furthermore, untargeted metabolomic analysis revealed that diterpenoids and apocarotenoids are preferentially accumulated in the DPE genotype in comparison with NPE, which may be linked to the divergence between the genotypes concerning PE biosynthesis, since sesquiterpenoids showed greater abundance in the NPE.Utility of the resourceThe LC‐HRMS files, publicly available in the MassIVE database (identifier MSV000092920), are valuable as they expand our understanding of PE biosynthesis, which can assist in the development of molecular strategies to reduce PE levels in toxic genotypes, making possible the food use of the seedcake, as well as its potential to contain high‐quality spectral information about several other metabolites that may possess biological activity.
A meta‐analysis revealing the technical, environmental, and host‐associated factors that shape the gut microbiota of Atlantic salmon and rainbow trout
Authors: Cao Shuowen , Dicksved Johan , Lundh Torbjörn , Vidakovic Aleksandar , Norouzitallab Parisa , Huyben David 
Published: April 2024

AbstractSalmonids, specifically Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss), are commonly farmed and their gut microbiota plays important roles for optimal growth, health, and physiology. However, differences in experimental design, technical factors and bioinformatics make it challenging to compare the results from different studies and draw general conclusions about their influence on the fish gut microbiota. For a more comprehensive understanding of the gut microbiota, we collected all the publicly accessible 16S rRNA gene sequencing data with clearly stated sample metadata from freshwater Atlantic salmon and rainbow trout intestinal contents and mucosa sequenced on the Illumina MiSeq platform. A total of 783 samples from 19 published studies were included in this meta‐analysis to test the impact of the technical, environmental, and host‐accociated factors. This meta‐analysis revealed that all the tested factors significantly influenced the alpha and beta diversities of the gut microbiota of salmon and trout. Technical factors, especially target region and DNA extraction kit, affected the beta diversity to a larger extent, while host‐associated and environmental factors, especially diet and initial fish weight, had a higher impact on the alpha diversity. Salmon had a higher alpha diversity and higher abundance of Enterococcus and Staphylococcus than trout, which had higher abundance of Weissella and Mycoplasma. The results of this meta‐analysis fill in a critical knowledge gap that demonstrate technical methodologies must be standardized and factors associated with host and environment need to be accounted for in the future design of salmonid gut microbiota experiments.
Supplementation of rumen‐protected lysine during the close‐up period improves vaginal discharge clearance in Holstein dairy cows
Authors: Kawashima Chiho , Hayakawa Hina , Taniguchi Aki , Sugimoto Yusuke , Kusaba Nobuyuki , Yamagishi Norio , Goto Akira 
Published: April 2024

AbstractWe aimed to evaluate the effects of rumen‐protected lysine (RPL) supplementation during the close‐up period on uterine involution and the resumption of ovarian function in dairy cows. Fifty‐two multiparous Holstein cows were categorized based on parity and expected calving date and randomly assigned to the RPL or control (CON) groups. The RPL group received 80 g of RPL daily from day 21 before the expected calving date until parturition. Blood samples were obtained twice weekly from pre‐supplementation to 6 weeks postpartum. The onset of luteal activity postpartum was determined via ultrasonography twice weekly for up to 6 weeks postpartum. Uterine involution was tracked at 3 and 5 weeks postpartum through the vaginal discharge score, percentage of polymorphonuclear cells (PMN) in endometrial cytology samples, presence of intrauterine fluid, and gravid horn diameter via ultrasonography. Before supplementation, the RPL group showed amino acid imbalance, which was improved by RPL supplementation. There were no significant differences in the onset of luteal activity, percentage of PMN, intrauterine fluid, or the diameter of the uterine horn between the two groups. The vaginal discharge score in the RPL group decreased from 3 to 5 weeks postpartum, whereas that in the CON groups did not decrease. The number of cows with clinical endometritis was lower in the RPL group. Overall, RPL supplementation during the close‐up period enhanced vaginal discharge clearance, potentially averting clinical endometritis, but did not affect the first ovulation in dairy cows.
Risk Factors of the Occurrence and Treatment Failure of Refractory Peritoneal Dialysis‐Associated Peritonitis: A Single‐Center Retrospective Study From China
Authors: Liang Qichen , Zhao Huiping , Wu Bei , Niu Qingyu , Lu Lixia , Qiao Jie , Men Chuncui , He Yuting , Chu Xinxin , Zuo Li , Wang Mei 
Published: April 2024

ABSTRACTBackgroundThis study aimed to investigate the clinical characteristics and prognosis of refractory peritoneal dialysis (PD)‐associated peritonitis as well as the risk factors of its occurrence and treatment failure.MethodsA single‐center retrospective cohort study was conducted among 519 patients undergoing PD from January 2007 to October 2021. According to the International Society for Peritoneal Dialysis guidelines, all episodes occurred in our center were divided into two groups: refractory and nonrefractory. Demographic, biochemical, and pathogenic bacteria and treatment outcome data were collected.ResultsDuring the 15‐year period, 282 episodes of peritonitis occurred in 166 patients undergoing PD. The refractory rate was 34.0% (96/282). Gram‐positive organisms were the leading cause of peritonitis (47.9%); however, gram‐negative organisms were predominant in refractory peritonitis (34.4%, p = 0.002). Multiple logistic regression revealed that gram‐negative organism‐based peritonitis, longer PD duration, and female sex were the significant independent predictors of refractory peritonitis. Among 96 refractory episodes, white blood cell (WBC) count, dialysate WBC on Day 3, and PD duration ≥5 years were the independent risk factors of treatment failure.ConclusionsGram‐negative organism‐based peritonitis, longer PD duration, and female sex were the independent risk factors of refractory peritonitis. Refractory peritonitis with higher WBC count, higher dialysate WBC on Day 3, and PD duration ≥5 years increased treatment failure risk and required immediate PD catheter removal. The timely identification of refractory peritonitis with high risk of treatment failure as well as timely PD catheter removal is important.
The role of emotional suppression and emotional beliefs in explaining the honor‐suicide link
Authors: Foster Stephen , Albright Austin , Bock Jarrod 
Published: April 2024

AbstractIntroductionPrior research has established individuals living in cultures of honor to be at higher risk for suicide. However, research has yet to examine how emotion regulation may play a role in explaining this link. The current research intended to address if those who endorse honor norms, who are keen to protect their reputation, might engage in emotional suppression as a way to avoid being seen as weak, thus heightening their risk for suicide.MethodData were collected using a survey design across three studies (total N = 988). Studies 1 and 2 were cross‐sectional designs with MTurk and undergraduate samples. Study 3 was conducted longitudinally using CloudResearch.ResultsResults demonstrated those higher in honor endorsement engage in emotional suppression to avoid being seen as weak (Study 1), that emotional suppression and subsequent depression indirectly explain the relationship between honor endorsement and suicide attempts (Study 2), and that relationships between honor endorsement and changes in suicidal ideation are explained by emotional suppression and depressive symptomology (Study 3).ConclusionFindings provide needed insight into the daily emotional experiences of honor endorsers and how it may influence suicidality, providing a point of entry for clinicians to construct meaningful honor‐oriented intervention programs.
Effects of high‐intensity interval training on depressive and anxiety symptoms in healthy individuals: A systematic review and meta‐analysis of randomized clinical trials
Authors: Gaia Jhonatan Wélington Pereira , Schuch Felipe Barreto , Ferreira Rodrigo Weyll , Souza Edielen de Lima , Ferreira Verônica Moreira Souto , Pires Daniel Alvarez 
Published: April 2024

AbstractAlthough evidence regarding the effects of high‐intensity interval training (HIIT) on mental health has increased in recent years, there is still no consensus regarding the effects of HIIT on the symptoms of depression and anxiety in a healthy population. Therefore, this systematic review aimed to investigate the effects of HIIT on depressive and/or anxiety symptoms in healthy individuals. The following four databases were searched: PubMed, Scopus, Embase, and PsycINFO. Only randomized clinical trials (RCTs) were included. We performed a random‐effects meta‐analysis based on standardized mean difference (SMD). The risk of bias was assessed using the RoB 2.0 tool, and the certainty of the evidence was evaluated based on recommendations GRADE. Eight RCTs evaluating 471 participants (81% female) were considered eligible for inclusion. The results of the meta‐analysis showed that HIIT‐based interventions had no significant effect on reducing anxiety (SMD = −0.17; 95% CI: −0.53, 0.19; p = 0.27) and depressive symptoms (SMD = −0.38; 95% CI: −1.06, 0.30; p = 0.17) compared with the passive control group. In conclusion, HIIT does not improve symptoms of depression and anxiety in healthy individuals. This finding is based on evidence of very low certainty. Therefore, the evidence is still not consistent enough to support HIIT as a viable strategy to reduce both outcomes because of the limited number of included studies and the overall quality of evidence.
Look over there. Where? A compositional approach to the modeling of public opinion on the most important problem
Authors: Jokinsky Steven , Lipsmeyer Christine S. , Philips Andrew Q. , Williams Laron K. , Whitten Guy D. 
Published: April 2024

AbstractObjectiveThis study aims to test whether the American public is polarized and/or parallel in its assessments of the most important problem.MethodsWe use compositional time series models and new data on public opinion to test for differences between subgroups.ResultsWe find inconsistent evidence of polarization for some issue areas but not others and remarkably robust evidence of parallel reactions across subgroups to economic and international shocks.ConclusionThe U.S. public is remarkably consistent in terms of its assessments of the most important problem and in how subgroups shift their perceptions of issue importance in reaction to changing circumstances.
A microRNA528‐ZmLac3 module regulates low phosphate tolerance in maize
Authors: Pei Laming , Gao Xiaomei , Tian Xiao , Liu Ning , Chen Minghao , Fernie Alisdair R. , Li Hui 
Published: April 2024

SUMMARYMicroRNAs are known to play a crucial role in plant development and physiology and become a target for investigating the regulatory mechanism underlying plant low phosphate tolerance. ZmmiR528 has been shown to display significantly different expression levels between wild‐type and low Pi‐tolerant maize mutants. However, its functional role in maize low Pi tolerance remains unknown. In the present study, we studied the role and underlying molecular mechanism of miR528 in maize with low Pi tolerance. Overexpression of ZmmiR528 in maize resulted in impaired root growth, reduced Pi uptake capacity and compromised resistance to Pi deficiency. By contrast, transgenic maize plants suppressing ZmmiR528 expression showed enhanced low Pi tolerance. Furthermore, ZmLac3 and ZmLac5 which encode laccase were identified and verified as targets of ZmmiR528. ZmLac3 transgenic plants were subsequently generated and were also found to play key roles in regulating maize root growth, Pi uptake and low Pi tolerance. Furthermore, auxin transport was found to be potentially involved in ZmLac3‐mediated root growth. Moreover, we conducted genetic complementary analysis through the hybridization of ZmmiR528 and ZmLac3 transgenic plants and found a favorable combination with breeding potential, namely anti‐miR528:ZmLac3OE hybrid maize, which exhibited significantly increased low Pi tolerance and markedly alleviated yield loss caused by low Pi stress. Our study has thus identified a ZmmiR528‐ZmLac3 module regulating auxin transport and hence root growth, thereby determining Pi uptake and ultimately low Pi tolerance, providing an effective approach for low Pi tolerance improvement through manipulating the expression of miRNA and its target in maize.