bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multicenter phase II study of CyclOBEAP regimen for elderly patients with poor‐prognosis aggressive lymphoma
Authors: Niitsu Nozomi , Okamoto Masataka , Kohori Mika , Aoki Sadao , Miura Ikuo , Hirano Masami 
Published: September 2006

AbstractWe treated elderly patients (65–69 years) who had aggressive lymphoma with the CyclOBEAP regimen, and we studied the safety and efficacy of this treatment. The CyclOBEAP regimen was administered over a total period of 12 weeks. Doxorubicin 40 mg/m2 was given every 2 weeks in combination with either cyclophosphamide 800 mg/m2 or etoposide 70 mg/m2 qd × 3. During the alternate weeks, non‐myelosuppressive vincristine 1.0 mg/m2 was given either with bleomycin 10 mg/m2 or alone. Prednisolone 40 mg/m2 was administered daily for three 14‐day periods during weeks 1–2, 5–6 and 9–10. There were 51 eligible patients. A complete response was achieved in 42 patients (82%). The 5‐year overall survival (OS) rate was 60.6% and progression‐free survival (PFS) rate was 51.8%. WHO grade 4 neutropenia was observed in 32 patients and thrombocytopenia in 8 patients. We showed that the CyclOBEAP regimen can be safely used in the treatment of aggressive lymphoma in elderly patients and it achieved a high rate of remission. Copyright © 2006 John Wiley & Sons, Ltd.
Biodegradable polyurethane cancellous bone graft substitutes in the treatment of iliac crest defects
Authors: Gogolewski Sylwester , Gorna Katarzyna 
Published: September 2006

AbstractPorous scaffolds were produced from newly designed biodegradable, segmented aliphatic polyurethanes of various chemical compositions and hydrophilic‐to‐hydrophobic segment ratios. The scaffolds were implanted into monocortical defects in the iliac crest of healthy sheep for 6 months. The resected cortex was not repositioned. The ilium defects, which were not implanted with polyurethane scaffolds, were used as controls. In none of the control defects was there bone regeneration at the time of euthanasia. The defects implanted with porous scaffolds from polyurethanes were healed to varying extents with cancellous bone. The structure of the regenerated cancellous bone was radiographically denser than the structure of native bone. New bone that was formed in the scaffolds with a higher amount of hydrophilic component contained more calcium phosphate deposit than the bone formed in the scaffolds with a lower amount of the hydrophilic component. There was no new cortex formed over the defect, but a thin layer of soft tissue covered the newly formed cancellous bone. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Conjugates of poly(DL‐lactide‐co‐glycolide) on amino cyclodextrins and their nanoparticles as protein delivery system
Authors: Gao Hui , Wang Yi‐Nong , Fan Yun‐Ge , Ma Jian‐Biao 
Published: September 2006

AbstractPoly(DL‐lactide‐co‐glycolide) (PLG) was chemically conjugated on two amino cyclodextrins, mono(6‐(2‐aminoethyl)amino‐6‐deoxy)‐β‐cyclodextrin and ethylenediamino bridged bis(β‐cyclodextrin), to afford novel amphiphilic conjugates. Those conjugates were then characterized with infrared spectrometry (IR), proton nuclear magnetic resonance (1H NMR) and gel permeation chromatography (GPC). A repeat‐nanoprecipitation (RP‐NP) method was also developed to fabricate the nanoparticles of the conjugates with a water‐soluble model protein, bovine serum albumin (BSA). At the end of RP‐NP process, the availability of BSA was over 80% while the entrapment efficiency was 40–50% for each nanoprecipitation. The nanoparticles were rigid and spherical with diameters of 110–180 nm determined by transmission electron microscope (TEM), atomic force microscopy (AFM) and particle size analyzer. Nanoparticles possessed good steric stability during freeze‐drying and resuspensions due to the existence of cyclodextrins corona. Interactions between BSA and the conjugates in the nanoparticles were then elucidated with IR experiments. About 25% BSA adsorbed on the surface of nanoparticles due to the interaction and was easy to release in the first day. The release of BSA from the nanoparticles was in three phases: a burst effect in the first day, a followed plateau in about a week, and a sustained release of the protein over 14 days. By changing the lactide/glycolide ratio, the degradation time of the conjugates and the release rate of BSA could be controlled. The loss of CDs content was faster than that of overall Mw during degradation since CDs formed outer corona of the nanoparticles. Both the novel biomaterials and the nanosphere fabrication technique contributed to the maintenance of protein structure. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
PSM/SH2‐B distributes selected mitogenic receptor signals to distinct components in the PI3‐kinase and MAP kinase signaling pathways
Authors: Deng Youping , Xu Hu , Riedel Heimo 
Published: September 2006

AbstractThe Pro‐rich, PH, and SH2 domain containing mitogenic signaling adapter PSM/SH2‐B has been implicated as a cellular partner of various mitogenic receptor tyrosine kinases and related signaling mechanisms. Here, we report in a direct comparison of three peptide hormones, that PSM participates in the assembly of distinct mitogenic signaling complexes in response to insulin or IGF‐I when compared to PDGF in cultured normal fibroblasts. The complex formed in response to insulin or IGF‐I involves the respective peptide hormone receptor and presumably the established components leading to MAP kinase activation. However, our data suggest an alternative link from the PDGF receptor via PSM directly to MEK1/2 and consequently also to p44/42 activation, possibly through a scaffold protein. At least two PSM domains participate, the SH2 domain anticipated to link PSM to the respective receptor and the Pro‐rich region in an association with an unidentified downstream component resulting in direct MEK1/2 and p44/42 regulation. The PDGF receptor signaling complex formed in response to PDGF involves PI 3‐kinase in addition to the same components and interactions as described for insulin or IGF‐I. PSM associates with PI 3‐kinase via p85 and in addition the PSM PH domain participates in the regulation of PI 3‐kinase activity, presumably through membrane interaction. In contrast, the PSM Pro‐rich region appears to participate only in the MAP kinase signal. Both pathways contribute to the mitogenic response as shown by cell proliferation, survival, and focus formation. PSM regulates p38 MAP kinase activity in a pathway unrelated to the mitogenic response. J. Cell. Biochem. 100: 557–573, 2007. © 2006 Wiley‐Liss, Inc.
Immediate early gene IEX‐1 induces astrocytic differentiation of U87‐MG human glioma cells
Authors: You Fukka , Osawa Yosuke , Hayashi Shin‐ichiro , Nakashima Shigeru 
Published: September 2006

AbstractThe immediate early response gene IEX‐1 is involved in the regulation of apoptosis and cell growth. In order to increase the apoptotic sensitivity to chemotherapeutic drugs and γ‐ray, we attempted to establish U87‐MG human glioma cell line expressing IEX‐1. Unexpectedly, however, transfection of IEX‐1 into U87‐MG glioma cells resulted in morphological changes to astrocytic phenotype and increase in glial differentiation marker proteins, S‐100 and glial fibrillary acidic protein (GFAP). Glial cell differentiation was used to examine in rat C6 glioma cell line, since this cell line express astrocytic phenotypes by increase in intracellular cAMP concentration. Stimulation of human U87‐MG glioma cells by membrane‐permeable dibutyryl cAMP (dbcAMP) not only elicited their morphological changes but also induced expression of IEX‐1 as well as S‐100 and GFAP. H89, an inhibitor of protein kinase A (PKA), blocked dbcAMP‐induced morphological changes of U87‐MG cells and expression of IEX‐1. In contrast, morphological changes and expression of S‐100 and GFAP induced by IEX‐1 were not affected by H89. Morphological changes induced by dbcAMP were totally abolished by functional disruption of IEX‐1 expression by anti‐sense RNA. These results indicate that IEX‐1 plays an important role in astrocytic differentiation of human glioma cells and that IEX‐1 functions at downstream of PKA. J. Cell. Biochem. 100: 256–265, 2007. © 2006 Wiley‐Liss, Inc.
Upregulations of Gata4 and oxytocin receptor are important in cardiomyocyte differentiation processes of P19CL6 cells
Authors: Uchida Shizuka , Fuke Satoshi , Tsukahara Toshifumi 
Published: September 2006

AbstractOxytocin induces P19 cells to differentiate into cardiomyocytes possibly through the oxytocin/oxytocin receptor system. We added oxytocin to the growth medium of P19CL6, a subline of P19, but they did not differentiate into cardiomyocytes as indicated by RT‐PCR and Western blotting results. During the cardiac commitment time of P19CL6 cells, the mRNA expression levels of the oxytocin receptor were upregulated by the addition of oxytocin as well as DMSO, but an upregulation of Gata4 expression levels was only observed for the cells induced by DMSO. The in silico analysis of the upstream sequence of the oxytocin receptor predicted putative binding sites for Gata4 and Nkx2.5. These results suggest that upregulations of the oxytocin receptor and Gata4 are important for cardiomyocyte differentiation processes. J. Cell. Biochem. 100: 629–641, 2007. © 2006 Wiley‐Liss, Inc.
Lower extremity arterial thrombosis following sonographically guided thrombin injection of a femoral pseudoaneurysm
Authors: Stawicki Stanislaw P. , Hoey Brian A. 
Published: September 2006

AbstractSonographically guided percutaneous thrombin injection is a minimally invasive and highly successful treatment of arterial pseudoaneurysms. Despite a very low complication rate, several severe arterial thrombotic events have been reported following thrombin injection of pseudoaneurysms. Native arterial thrombosis, though recognized as a severe complication of thrombin injection, has not been well described in the literature. We report a case of successful surgical management of arterial thrombosis after percutaneous thrombin injection of a femoral artery pseudoaneurysm in a 69‐year‐old woman. The pathophysiology, risk factors, diagnosis, and treatment options for this complication are discussed. © 2006 Wiley Periodicals, Inc. J Clin Ultrasound, 2006.
Relation of cerebral small‐vessel disease and brain atrophy to mild parkinsonism in the elderly
Authors: Reitz Christiane , Trenkwalder Claudia , Kretzschmar Konrad , Roesler Andreas , v. Eckardstein Arnold , Berger Klaus 
Published: September 2006

AbstractThe association between cerebral small‐vessel disease, brain atrophy, and the risk and severity of mild parkinsonian signs (MPS) remains unclear. The objective of this study is to examine the effect of lacunar brain infarcts, cerebral white matter lesions (WMLs), and cortical atrophy on the risk and severity of MPS. This study is a cross‐sectional community‐based cohort study comprising 268 subjects, 65 to 83 years of age, residing in the Augsburg region of southern Germany, and without contraindications for magnetic resonance imaging (MRI) of the brain. Main outcome measures. Subcortical and periventricular WMLs, lacunar brain infarcts, and cortical atrophy determined using a standardized MRI protocol developed for the Rotterdam Scan Study and an established rating scale. MPS, assessed in a standardized neurological examination and based on the Unified Parkinson's Disease Rating Scale motor scale. Lacunar brain infarcts and large subcortical white matter lesions were associated with an elevated risk of resting tremor. More severe cortical atrophy was related to an increased risk of rigidity and bradykinesia. In a linear regression analysis relating each individual MRI measurement with the severity of MPS, the number of lacunar brain infarcts and the degree of brain atrophy were correlated with the severity of resting tremor, whereas the size of subcortical and periventricular WMLs was correlated with the severity of rigidity. A higher degree of brain atrophy was associated with increased severity of either cardinal sign. In our study, presence and volume of lacunar brain infarcts, cerebral WMLs, and cortical atrophy were associated with the risk as well as severity of MPS. Determining the presence of these brain changes using brain imaging might contribute to identify persons at risk for MPS. © 2006 Movement Disorder Society
ABO incompatible stem cell transplantation in children does not influence outcome
Authors: Helming A.M. , Brand A. , Wolterbeek R. , van Tol M.J.D. , Egeler R.M. , Ball L.M. 
Published: September 2006

AbstractBackgroundAlthough delayed red cell engraftment and/or hemolysis have been thoroughly documented in association with ABO incompatibility between donor and recipient in patients undergoing hematopoietic stem cell transplantation (HSCT), there are no studies defining the general, long term clinical outcome in a large group of pediatric patients.MethodsWe undertook a retrospective single center analysis of children undergoing pediatric allogeneic stem cell transplantation to determine the influence of ABO donor/recipient incompatibility. Outcome was analyzed according to donor type and included survival, graft versus host disease (GvHD), relapse, days of infection, antibiotic use, transfusion requirement and duration of hospital stay.ResultsTwo hundred and sixteen children (136 males; 80 females, aged 0–19) transplanted between January 1992 and December 2003 were included in the study. Indications for transplantation were hematological malignancies (n = 179) and aplastic conditions (n = 37). ABO compatibility was documented in 121 donor/recipient pairs. ABO incompatibility was documented in 95 donor/recipient pairs with 40 major, 40 minor and 15 bi‐directional incompatible pairs. ABO incompatibility did not influence survival rate (P = 0.3762), the incidence of GvHD (P = 0.253) or rate of relapse (P = 0.930). Recovery of leucocytes was influenced by ABO incompatibility (P = 0.0493), but the rate of infection, transfusion requirements and duration of hospital stay are not.ConclusionIn the pediatric setting, ABO major and/or minor mismatch between donor and recipient did not significantly influence the outcome of HSCT. The choice of donor should be determined by the degree of HLA match and CMV status in preference to ABO blood group compatibility. Pediatr Blood Cancer 2007;49:313–317. © 2006 Wiley‐Liss, Inc.
Audit of staff knowledge of the correct management of hypoglycaemia in a busy teaching hospital
Authors: Furness C , Baker Helen , Hammersley M , Pal A 
Published: September 2006

AbstractHypoglycaemia is a common and potentially serious complication in the treatment of diabetes that is frequently encountered in a hospital environment. A basic understanding of the recognition and immediate treatment of a patient experiencing a hypoglycaemic episode is key to appropriate management. An audit of staff knowledge of appropriate treatment of a hypoglycaemic episode (using a simple questionnaire) was carried out in the John Radcliffe Hospital, Oxford, with the aim of comparing knowledge in area A (wards in the hospital where diabetes education and diabetes specialist nurse input are available) and area B (wards where these services are not yet in place). The mean questionnaire score in area A was 65.6% compared to 45% in area B. Thirty‐three percent of area A nursing staff had attended a diabetes education session in the last year; the mean score for nurses who had attended such a session was 86.7% compared to 62.8% for those who had not. These results have led us to conclude that knowledge was better in areas where diabetes support services are in place and in individuals who have received training. Area B represents a potential target for the implementation of such services, and the continued expansion of the diabetes specialist nurse department in the John Radcliffe is expected to lead to such changes. Currently we do not feel that all hypoglycaemic episodes are being managed appropriately and would encourage widespread education and training for all staff potentially involved in the care of these patients. Copyright © 2006 John Wiley & Sons, Ltd.
What education do people with type 1 diabetes want and how should we provide it?
Authors: Woodward A , Dawes S , Dolan D , Wallymahmed M 
Published: September 2006

AbstractThe National Service Framework advised that continuing education is an essential component of diabetes care and is the cornerstone of treatment for people with diabetes.An education needs questionnaire was used to survey 100 people with type 1 diabetes attending consultant clinics in a north Liverpool teaching hospital. The aim of the survey was to obtain the views on patient education which would enable us to develop a new patient‐centred education programme.The questionnaire was given to 100 consecutive people with type 1 diabetes attending both medical and nurse consultant clinics. Newly diagnosed people with diabetes under one year's duration were excluded. The questionnaire was divided into key areas including diabetes core topics, previous education, views on further education and open comments.There were 95 (95%) completed questionnaires, 52 (55%) completed by women, and the mean (SD) age was 39±14 (range 17–82 years). Eighty‐four (88%) people remembered receiving previous education but 58 (61%) wanted further education on most of the listed topics.As a result of this survey we have developed an education care pathway for people with newly diagnosed diabetes which will provide initial education and self‐care skills. The next step is to design a more advance programme, for all interested type 1 patients to develop further self‐management skills, with specific information on microvascular and macrovascular disease. Copyright © 2006 John Wiley & Sons, Ltd.
Therapeutic profile of orphan medicines
Authors: de Barros Carla Maria Teixeira , Papoila Ana Luísa 
Published: September 2006

AbstractPurposeTo characterise the therapeutic profile of orphan medicines.Materials and MethodsA cross‐sectional study was performed during 2 months in a convenience sample of seven hospital pharmacy services, in the region of Lisbon. Data were collected, from pharmaceutical service's records.ResultsA total of 18 orphan medicines, were dispensed to 355 patients with rare diseases. Most patients were adults (76.4%). Premature and neonates accounted with 50.0% of the paediatric patients. Differences were not found between the proportion of male and female patients across age groups (p = 0.762). Only 18.3% were inpatients. A high proportion of paediatric inpatients (58.3%) were seen in relation to adult inpatients (5.9%) (p = 0.001).In general, anti‐neoplastic and immunomodulating agents for rare cancers were the most frequent dispensed medicines (51.3%). In relation to paediatrics, Caffeine Citrate for primary apnoea of premature newborns had the higher frequency distribution (57.1%). Five (71.4%) medicines dispensed for paediatrics, do not have market authorisation and the remaining (28.6%) were used off‐label.For pulmonary arterial hypertension 19 of 27 patients (70.3%) were treated with Bosentan. According to evidence‐based clinical practice guidelines, Bosentan has a good level of evidence and substantial benefit: grade of recommendation A.ConclusionsMost orphan medicines dispensed to paediatrics and adults were not licensed.A special pharmacovigilance program and a risk management plan through the entire life cycle should be implemented, towards effectiveness and safety of orphan medicines. Copyright © 2006 John Wiley & Sons, Ltd.
Trends in consumption of psychiatric drugs in Serbia and Montenegro 2000–2004
Authors: Divac Nevena , Toševski Dušica Lečić , Babić Dragan , Djurić Dušan , Prostran Milica , Samardžić Ranka 
Published: September 2006

AbstractPurposeTo investigate the trends in psychiatric drugs utilization in Serbia and Montenegro over the 5‐years period (2000–2004).Materials and methodsThe wholesale data issued by the manufacturers or distributors were used. Also, the lists of drugs used from the Institute of Mental Health (institution which provides primary, secondary and tertiary psychiatric care) were used to corroborate the wholesale data. The utilization of drugs in general population was calculated using ATC/DDD methodology, and was expressed in Defined Daily Doses (DDD) per 1000 inhabitants per day. The utilization of drugs in the Institute of Mental Health was calculated from the hospital pharmacy records and was expressed as the number of DDDs per 100 bed‐days. The statistics was performed using standard statistical methods.ResultsThe general consumption of psychiatric drugs in Serbia and Montenegro significantly increased during the 2000–2004 period (from 45.1 DDD/1000 inhabitants/day in 2000 to 69.1 DDD/1000 inhour/day in 2004). The general utilization of anxiolytic/hypnotic drugs surged significantly, but the inhospital use remained rather stable. The most prominent changes were noted in the utilization of antidepressants, which doubled in that period, both in general and inhospital settings. Among antipsychotics in general population, there is a significant increase in the utilization of clozapine. In inhospital setting, there is a significant increase of total utilization of antipsychotic drugs, but this is also mainly on the account of atypicals.ConclusionsThe total consumption of psychiatric drugs significantly increased. The utilization of anxiolytic/hypnotic drugs in 2004 reached 60 DDDs/1000 inhabitants/day, which is in collision with the trend in developed countries. It is necessary to form national guidelines for prescribing anxiolytic/hypnotic drugs, in order to rationalize their use. More favourable is the antidepressant (SSRIs in particular) and atypical antipsychotic drugs utilization increase. Copyright © 2006 John Wiley & Sons, Ltd.
Effect of nano‐SiC dispersion on thermoelectric properties of Bi2Te3 polycrystals
Authors: Li Jing‐Feng , Liu Jing 
Published: September 2006

AbstractNano‐SiC dispersed Bi2Te3 was synthesized by mechanical alloying (MA) followed by spark plasma sintering (SPS). The effects of SiC dispersion on the thermoelectric properties of the SPS‐sintered Bi2Te3 alloys were investigated. The results revealed that SiC dispersion in the Bi2Te3 matrix increased the absolute value of Seebeck coefficient. Although the electrical resistance was increased somewhat, Bi2Te3 with 0.1 wt% SiC showed a higher power factor than Bi2Te3 without SiC. Because the dispersion of SiC nano‐particles reduced the thermal conductivity at the same time, 0.1 wt% (∼0.24 vol% SiC) addition of SiC nano‐particles increased the figure of merit of Bi2Te3 by 18%. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Magnetization reversal in micron‐sized stripes of epitaxial (110) YFe2 films
Authors: Wang K. , Martin K. N. , Morrison C. G. , Ward R. C. C. , Bowden G. J. , de Groot P. A. J. 
Published: September 2006

AbstractArrays of micron‐sized stripes of epitaxial (110) Laves phase YFe2 films have been fabricated using a UV laser direct write system and etching process. The magnetization reversal of the stripe arrays in the sample plane is investigated by magneto‐optical Kerr magnetometry. The measured MO Kerr hysteresis loops reveal that a uniaxial magnetic anisotropy can be induced in the materials by the shape of the stripes. The easy axis is found to be rigidly confined parallel to the length axis of the stripe and the hard axis is perpendicular to it. This is correlated with the induced uniaxial anisotropy which dominates the intrinsic four‐fold magnetocrystalline anisotropy of the cubic Laves phase structure. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)