Characteristics of HIF-1Α and HSP70 MRNA Expression, Level, and
Interleukins in Experimental Chronic Generalized Periodontitis
Authors: Daria Parkhomenko , Igor Belenichev , Oleh Kuchkovskyi , Victor Ryzhenko 
Published: April 2024

Objectives:
Periodontal diseases are a rather complex problem of modern dentistry and do not have only medical
but also social significance.

:
The objective of this study is to weigh the effect of a mixture of Thiotriazoline and L-arginine (1:4) on the parameters of
the system of endogenous cytoprotection of blood and periodontal illness in rats with experimental chronic generalized
periodontitis and substantiate further study of this blend.

Materials and Methods:
The study aimed to evaluate the impact of a combination of Thiotriazoline and L-arginine (in a
ratio of 1:4) on the parameters of the endogenous blood cytoprotection system and periodontium in rats with experimental
chronic generalized periodontitis. A group of outbred rats weighing 190-220 g and sourced from the vivarium of the Institute
of Pharmacology and Toxicology of the Academy of Medical Sciences of Ukraine were divided into four groups, each
consisting of 10 animals.

:
(1) Intact group, animals that were injected intragastrically with a solution of sodium chloride to chloride 0.9% for 30 days.
(2) control, animals with experimental CGP who intragastrically sodium chloride solution 0.9% for 30 days. (3) animals
with experimental CGP were injected intramuscularly with Thiotriazoline + L-arginine (1:4) in a dosage of 200 mg/kg (30
days). (4) animals with experimental CGP, for which daily intragastric reference drug Mexidol, in dosage 250 mg/kg (30
days).

:
In this study, we utilized two substances: Thiotriazoline and L-arginine hydrochloride. The combination of Thiotriazoline
and L-arginine (in a ratio of 1:4) was prepared at the Department of Pharmaceutical Chemistry of ZSMU. At the conclusion
of the experiment, the rats were carefully removed from the study while under thiopental-sodium anesthesia, and administered
at a dosage of 40 mg/kg.

Results:
We have found that the administration of a combined preparation of Thiotriazoline with L-arginine to rats with CGP leads to a significant decrease in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a by 56.1% and 71%, respectively.

Conclusion:
The administration of Mexidol at a dosage of 250 mg/kg, as well as the combination of Thiotriazoline and Larginine
in a ratio of 1:4 at a dosage of 200 mg/kg, resulted in a significant reduction in gingival pocket depth in animals
with CGP. Specifically, the gingival pocket depth was reduced to 6 mm (p < 0.05) with Mexidol and further reduced to 4
mm (p < 0.05) with the combination of Thiotriazoline and L-arginine. Additionally, the animals exhibited minimal bleeding,
swelling, and tooth mobility when treated with the combination of Thiotriazoline and L-arginine.

:
The administration of a combination of Thiotriazoline and L-arginine (in a ratio of 1:4) at a dosage of 200 mg/kg to animals
with CGP resulted in a noteworthy reduction in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a. Specifically,
there was a significant decrease of 56.1% (p < 0.05) in IL-1b and 71% (p < 0.05) in TNF-a levels. The course administration
of a combination of Thiotriazoline and L-arginine (1:4) (200 mg/kg) to animals with CGP led to an increased expression
of HSP70 mRNA (p < 0.05) in the periodontium by 8.2 times and HIF-1a mRNA by 8.2 times. 2.8 times (p < 0.05) against the
background of an increase in the blood concentration of HSP70 by 95% (p < 0.05). Also, in the periodontium of animals in this
group, a decrease in the expression of c-Fos mRNA by 36.7% (p < 0.05) was found compared to the control group.