bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of unintended open reading frame ORF-130 expression in maize event MZIR098 by LC-MS as part of the allergenicity risk assessment of genetically modified crops
Authors: Birukou Ivan , Bailey Kathryn , Sugar James , McClain Scott , Young Scott 
Published: February 2024

Rigorous safety assessments are required for genetically modified (GM) crops to support their use as food or feed. These assessments include bioinformatics analysis of the amino acid sequences of all potential open reading frames (ORFs) encoded in the exogenous DNA introduced into the plant through genetic modification. Sequence similarities of potential DNA products to known or potential toxins and allergens trigger subsequent assessments of potential hazards for the consumer or the environment. The bioinformatic analysis of Syngenta maize event MZIR098 insert DNA revealed a total of 415 ORFs. One putative ORF (ORF-130) triggered further risk assessment based on its sequence similarities with known allergens, including peanut allergen Ara h 1, wheat glutenin, and others. We examined the potential expression of ORF-130 at a protein level to exclude the possibility of exposure during consumption of MZIR098 maize. Specifically, we developed and validated a liquid chromatography approach coupled to a tandem mass spectrometry (LC-MS/MS) method, called parallel reaction monitoring (PRM). This measure of expression was quantitative and targeted against the peptides unique to ORF-130. The linear range of the method was 25-5000 amol ORF-130/µl. Both, limit of detection (LOD) and lower limit of quantitation (LLOQ) of the method, were 12 μg ORF-130 per gram of dry MZIR098 maize leaf. The method was accurate and specific to ORF-130. PRM analysis of MZIR098 extracts did not detect putative ORF-130 above the limits of detection/quantitation. Furthermore, the broad proteomic data dependent acquisition (DDA) analysis of the MZIR098 extracts demonstrated no evidence of ORF-130 corroborating the PRM analysis. Our results confirm that ORF-130 is not characterized as a functional gene in Syngenta maize event MZIR098 insert DNA and does not express gene products at detectable levels.
Cough in children: features of diagnosis and choice of therapy
Authors: Malanicheva T. G. , Ziatdinova N. V. , Kuznetsova O. Yu. , Ismagilova D. R. 
Published: February 2024

Introduction. Cough is the most common symptom of acute respiratory infections, which results from inflammation of the upper and lower respiratory tract. Herbal medicines that include an ivy leaf extract preparation are widely used for the treatment of cough.Aim. To evaluate the effectiveness and safety of the ivy leaf extract preparation as a syrup in young children with acute bronchitis. Materials and methods. We observed 44 children with acute bronchitis aged 4 months up to 3 years. The main group included 24 children receiving the ivy leaf extract preparation as part of complex therapy: 2.5 ml once a day for under 1-year children, and 2.5 ml 3 times a day for children aged 1–3 years for 7–10 days. The comparison group included 20 children receiving ambroxol at an age-appropriate dose as part of complex therapy. The complex therapy for bronchitis did not differ in the groups to be compared. Clinical effectiveness was assessed by analysis of the changes in the main symptoms of the disease.Results and discussion. It was found that the intake of the ivy leaf extract preparation showed high clinical effectiveness in 91.6% of cases, which included rapid relief of the main symptoms of the disease (cough, wheezing in the lungs), a 1.7-fold reduction of the exacerbation period, a 1.8-fold decrease of the cough period. Prescription of systemic antibiotics for children with acute bronchitis was required in 12.5% patients of the treatment group, and in 25% patients of the comparison group. The overall assessment of tolerability of the ivy leaf extract preparation was rated as excellent and good in 95.8% of cases.Conclusion. Due to high clinical effectiveness and safety of the ivy leaf extract preparation, it can be recommended for common practical use in acute bronchitis children from the first days of life.
Supply of vitamins (A, E, D, C, B6, B12) and mineral substances (ZN, FE, MG, CA, P) for children with recurrent respiratory infections and deficiency correction of their deficiency as a possibility to prevent frequent respiratory infections
Authors: Safina A. I. , Zakirov I. I. , Mansurova G. Sh. , Yusupova N. Z. , Iskandirova V. A. 
Published: February 2024

Introduction. Recurrent respiratory infections in children are a pressing problem in pediatrics. To maintain and function the immune system in children, their provision of vitamins and minerals is important.Purpose. To assess the provision of children with recurrent respiratory infections with vitamins (A, E, D, C, B6, B12, folic acid) and minerals (Zn, Fe, Mg, Ca, P) and to correct their deficiency with a vitamin-mineral complex.Materials and methods. The study was conducted on 65 children aged 3 to 8 years in 2 groups of children: group 1, children with RID, n = 50; group 2 – control, n = 15). An outpatient examination, a parent survey and a blood test for vitamins (A, E, D, C, B6, B12, folic acid) and minerals (Zn, Fe, Mg, total Ca, Ca++, P) were carried out. 30 children with RID were prescribed the vitamin and mineral complex, 1 tablet 2 times a day, with an assessment of vitamin and mineral sufficiency and the frequency of respiratory infections after the end of the dose.Results. In all examined children, both in the main and control groups, the most common were deficiencies of fat-soluble vitamins D (69%), A (40%), E (35%) and the minerals Zn (70%) and Fe (44%). In children with RID, deficiency of vitamins D, A and Zn was more common than in the control group (p < 0.05), in 96% of cases it was combined, more often in the form of a combined deficiency of fat-soluble vitamins D, A, E and minerals Zn, Fe (66% of cases). Taking vitamin-mineral complex in the 2nd prophylactic dose for 1 month contributed to an improvement in vitamin and mineral sufficiency and a decrease in respiratory morbidity in the next 2 months after stopping the drug.Conclusion. Vitamin-mineral complex can be successfully used to maintain vitamin and mineral levels and reduce respiratory morbidity in children with recurrent respiratory infections.
Obesity and abnormal uterine bleeding in adolescent girls: is there a relationship?
Authors: Nikitina I. L. , Liskina A. S. , Kelmanson I. A. , Kuptsova P. N. 
Published: February 2024

Introduction. Obesity is a non-communicable pandemic and a major problem worldwide. As the prevalence of childhood obesity increases, there is growing evidence of an association between obesity and risk factors for menstrual disorders.Aim. To study the role of obesity treatment in shaping menstrual disorders in adolescent girls.Materials and methods. Group: with obesity and menstrual cycle disorders. Group 2: obese and regular menstrual cycle. An assessment of anthropometric data, collection of hereditary history, life history, and analysis of data from menstrual cycle diaries were carried out. All girls were given recommendations aimed at reducing body weight, and after 12 months, weight dynamics and the nature of the menstrual cycle were assessed.Results. An increase in body weight for every 1 kilogram added over 12 months significantly increases the risk of menstrual cycle disorders: the odds ratio is 1.45 (95% CI: 1.11–1.88), p = 0.005. An increase in body mass index by 1 unit over 12 months significantly increases the risk of menstrual cycle disorders: the odds ratio is 1.70 (95% CI: 1.28–2.24), p = 0.001, similarly decreasing with a corresponding decrease in body mass index.Discussion. The risk of menstrual cycle disorders is associated with a dynamic change in body weight: with an increase in body weight during the year, the chances of menstrual cycle disorders for every 1 kg added increase by 1.45 times, decreasing by the same number of times with a corresponding decrease in weight. With an increase in body mass index for each added unit per year, the chances of menstrual cycle disorders increase by 1.7 times, decreasing by the same number of times with a corresponding decrease in body mass index.Conclusions. Dynamic changes in body weight can have both a positive and a negative effect on the prognosis of menstrual cycle disorders, which determines the great importance of therapeutic efforts aimed at reducing body weight.
Intestinal microbiota features of children born by cesarean section and its correction
Authors: Dedikova О. V. , Zakharova I.  N. , Kuchina A. E. , Berezhnaya I. V. , Sugian N. G. , Ardatskaya M. D. 
Published: February 2024

Introduction. Studying the gut microbiota of C-section newborns and its correction is a topical problem at present.Aim. To study the process of gut microbiota formation in healthy C-section infants, and the option for its correction using a Lactobacillus reuteri strain DSM 17938 probiotic.Materials and methods. A total of 80 healthy newborns were included in the study. Of these, 59 completed the study: the treatment group consisted of 36 elective caesarean (EC)-section infants and the control group was made up of 23 vaginally delivered infants. Newborns of the treatment study group were randomized into subgroups, one of which received the L. reuteri strain DSM 17938 (L subgroup) probiotic, and the second one did not receive it (0 subgroup). During the entire period of the study, infants were exclusively breastfed. The gut microbiota was analysed with the 16S rRNA sequencing method, and the metabolic activity of the gut microbiota was additionally assessed using gas-liquid chromatography.Results and discussion. The metagenomic analysis showed that the taxonomic richness and biological diversity of the gut microbiota in L subgroup infants increased in time interval 3, which indicated the active effect of the strain on the infant microbiota by Exposure Day 30. The gas-liquid chromatography analysis showed more pronounced changes in short-chain fatty acids in infants of the L subgroup: they were more similar to the findings in the control group (vaginal delivery). Also, bowel problems and allergies occurred less frequently in infants of this subgroup and they less frequently suffered from respiratory diseases during the year. The study showed the association between the mode of delivery and changes in anthropometric measurements.Conclusion. The use of L. reuteri strain DSM 17938 probiotic had a significant effect on the formation of the microbiota by the 30th day of age. A daily long-term addition of a L. reuteri strain DSM 17938 probiotic can prevent early microbiota dysbiosis and have a protective effect in later age period.
Fatores associados ao parto cesáreo em mulheres acompanhadas na atenção primária
Authors: Da Silva Thayane Leila Gonçalves , Rêgo Adriana  Sousa , Brito Andressa Pestana , Araújo Allanne Pereira , Dos Santos Alexsandro Ferreira , Costa Luciana Cavalcante , Silva Flor de Maria Araújo Mendonça , Barbosa Janaina Maiana Abreu 
Published: February 2024

O objetivo do estudo foi verificar as características antropométricas e os fatores associados ao parto cesáreo em mulheres acompanhadas na atenção primária em São Luís – MA. Trata-se de estudo de coorte, realizado com gestantes que realizaram o pré-natal em Unidades Básicas de Saúde (UBS) em São Luís – MA e acompanhadas até 42 dias após o parto. A análise estatística foi realizada no Stata® versão 16.0 no qual foi realizado a Regressão de Poisson, e para aceitação das associações investigadas no modelo final, foi adotado o valor de p < 0,05. Das 80 mulheres acompanhadas, 49,37% tiveram parto cesáreo, 5,33% iniciaram a gravidez desnutridas e 41,33% com excesso de peso no final da gestação, 14,47% estavam desnutridas e 46,5% estavam com excesso de peso. Os fatores associados ao parto cesáreo foram gravidez na adolescência (IRR: 2,26; IC: 1,20 - 4,25), nível de escolaridade baixo, sendo este, classificado em analfabetismo / fundamental I incompleto (IRR: 3,57; IC: 2,61- 4,88), já ter tido ≥ 1 gestações (IRR: 0,53; IC: 0,16-1,72), ter sintomas de ansiedade (IRR: 2,24; IC: 1,20 - 4,19) e hipertensão arterial (IRR: 5,75; IC: 1,18 – 2,81) durante a gravidez, ter iniciado a gravidez com desnutrição (IRR:6,79; IC:1,43-3,22) e ter obesidade no final da gestação (IRR: 2,64; IC: 1,11 - 6,26). É necessário o aprimoramento da assistência nutricional durante as consultas do pré-natal, com o objetivo de prevenir a desnutrição e excesso de peso, minimizando assim as complicações oriundas do estado nutricional.
Exploring data quality and seasonal variations of N2O in wastewater treatment: a modeling perspective
Authors: Hansen Laura Debel , Stentoft Peter Alexander , Ortiz-Arroyo Daniel , Durdevic Petar 
Published: February 2024

Abstract

In this work, operational data collected from four Danish wastewater treatment plants (WWTP) are assessed for quality issues and analyzed to investigate the feasibility of data-driven modeling for control purposes. All plants have permanent N2O sensors installed in the biological reactors, and N2O data are collected on the same terms as other operational data. We present and deploy a six-dimensional data quality assessment to the operational data evaluating (1) relevance, (2) accuracy, (3) completeness, (4) consistency, (5) comparability, and (6) accessibility. To increase the accuracy and completeness of the stored data, it is suggested that future initiatives are taken toward the collection and storing of metadata in WWTPs. Furthermore, seasonal variations and time-varying relationships between N2O, nitrogenous variables, and oxygen are investigated and compared across various case plants and process designs. Results show that the quality of the operational data varies substantially between plants. The investigation of time-varying interrelation between N2O and nitrogenous variables showed no clear pattern within or across different case plants. Furthermore, it is recommended that future research should consider adapting models so that more influence is linked to reliable measurements, contrary to assuming that all variables are of equal quality.
The Functions and Application Prospects of Hepatocyte Growth Factor in
Reproduction
Authors: Mi Xin , Chen Caiyi , Feng Chen , Qin Yingying , Chen Zi-Jiang , Yang Yajuan , Zhao Shidou 
Published: February 2024

Abstract:
Hepatocyte growth factor (HGF) is expressed in multiple systems and mediates a variety
of biological activities, such as mitosis, motility, and morphogenesis. A growing number of
studies have revealed the expression patterns and functions of HGF in ovarian and testicular physiology
from the prenatal to the adult stage. HGF regulates folliculogenesis and steroidogenesis by
modulating the functions of theca cells and granulosa cells in the ovary. It also mediates somatic
cell proliferation and steroidogenesis, thereby affecting spermatogenesis in males. In addition to
its physiological effects on the reproductive system, HGF has shown advantages in preclinical
studies over recent years for the treatment of male and female infertility, particularly in women
with premature ovarian insufficiency. This review aims to summarize the pleiotropic functions of
HGF in the reproductive system and to provide prospects for its clinical application.
Uncommon Metachronicity: Breast and Endometrial Carcinomas in a Young Woman with No Familial Cancer History - A Case Report
Authors: Kumar Naina , Palo Seetu , Sinha Aadil , Mangla Mishu 
Published: February 2024

Background:
Worldwide, breast and endometrial carcinomas are the two most common
cancers of women, but their coexistence in a woman <40 years of age is a rare event. The risk of developing
a new second primary cancer is higher in cancer survivors compared to those without a history
of cancer. The exact pathogenesis of two different primary malignancies is not clear. Still, the
coexistence of breast and endometrial carcinoma may be due to germline mutations of p53, BRCA 1,
and BRCA 2 genes, environmental, hereditary, and immune-mediated factors.

Case Presentation:
The present case report briefs the finding of a 37-year-old woman who presented
to the Gynecological outpatient department with complaints of abnormal uterine bleeding and a solid
irregular lump in the upper outer quadrant of the right breast and the right axilla. She had a history of
modified radical mastectomy for left breast ductal carcinoma five years back. She was diagnosed as a
case of metachronous endometrial carcinoma (stage IA) with a recurrence of invasive ductal carcinoma
in the right breast with metastasis to the right axillary lymph node.

Conclusion:
The metachronous development of breast and endometrial carcinoma in young women
is relatively rare. In young patients <40 years of age with gynecological or breast carcinoma, a thorough
work-up, including genetic testing and follow-up, should be done for early diagnosis of metachronous
or synchronous primary malignancies of other organs.
MRI-based Texture Analysis in Differentiation of Benign and Malignant
Vertebral Compression Fractures
Authors: Karabay Nuri , Odaman Huseyin , Vahaplar Alper , Kizmazoglu Ceren , Kalemci Orhan 
Published: February 2024

Introduction::
The diagnosis and characterization of vertebral compression fractures are very important for clinical management. In this evaluation, which is
usually performed with diagnostic (conventional) imaging, the findings are not always typical or diagnostic. Therefore, it is important to have new
information to support imaging findings. Texture analysis is a method that can evaluate information contained in diagnostic images and is not
visually noticeable. This study aimed to evaluate the magnetic resonance images of cases diagnosed with vertebral compression fractures by the
texture analysis method, compare them with histopathological data, and investigate the effectiveness of this method in the differentiation of benign
and malignant vertebral compression fractures.

Methods::
Fifty-five patients with a total of 56 vertebral compression fractures were included in the study. Magnetic resonance images were examined and
segmented using Local Image Feature Extraction (LIFEx) software, which is an open-source program for texture analysis. The results were
compared with the histopathological diagnosis.

Results::
The application of the Decision Tree algorithm to the dataset yielded impressively accurate predictions (≈95% in accuracy, precision, and recall).

Conclusion::
Interpreting tissue analysis parameters together with conventional magnetic resonance imaging findings can improve the abilities of radiologists,
lead to accurate diagnoses, and prevent unnecessary invasive procedures. Further prospective trials in larger populations are needed to verify the
role and performance of texture analysis in patients with vertebral compression fractures.
Prevalence and Related Factors of Dizziness Among Older Adults: A
Cross-Sectional Study in Ardakan City, Iran
Authors: Morowatisharifabad Mohammad Ail , Sharifabad Mahdieh Hosseini , Aboutorabi-zarchi Marzieh 
Published: February 2024

Background:
Dizziness and balance disorders are directly related to aging in humans-
Dizziness is one of the most important factors causing the burden of disability after the age of 65.
Bearing in mind the increase in the elderly population in Iran and the world and the prevalence of
dizziness in old age, early diagnosis of dizziness and determination of the factors affecting its
severity facilitate its treatment and are effective in preventing its adverse consequences.

Objective:
To determine the prevalence of dizziness and its related factors among older adults in
Ardakan city, Yazd province, Iran, in 2022.

Methods:
This cross-sectional study was conducted in four comprehensive health centers of Ardakan
city with the participation of 260 elderly people aged ≥60 years, who were randomly included
in the study. Data were collected using a series of questionnaires which were completed by interviewing
the participants. The variables of this study included demographic information, information
related to the dizziness status, diseases, medications, use of mobility aids, physical activity
level, fear of falling, quality of life and depression.

Results:
The prevalence of dizziness among older adults of Ardakan city was 48.5%. In terms of
the severity of dizziness, 38.8% had substantial dizziness, and 9.6% had mild dizziness. Dizziness
was significantly related to physical activity (p<0.05), fear of falling (p <0.01), depression (p
<0.05), history of falling (p <0.01), use of mobility aids (p <0.01), age (p<0.01), education level
(p<0.01), gender (p <0.05) and diseases such as high blood pressure (p<0.05), hypothyroidism (p
<0.01) and ear diseases(p <0.01). Also, elderly people with dizziness used significantly more medications
such as sedatives (p<0.01), antihypertensive drugs (p <0.05) and cytotoxic drugs (p
<0.01).

Conclusion:
About half of the older adults experience dizziness, and this problem is associated
with depression, fear of falling, history of falling, low physical activity, age, female gender, ear
diseases, high blood pressure, and hypothyroidism. In addition, the use of medications such as anti-
hypertensives, sedatives and cytotoxic drugs is related to dizziness. Families with elderly people,
doctors and healthcare workers need to be educated and pay more attention to the above.
Immunomodulator Drugs in Unani Medicine: Evidence from Preclinical
and Clinical Research
Authors: Hamiduddin  , Shah Sabeena , Itrat Malik , Zaigham  , Ahmed Uzair 
Published: February 2024

Abstract::
The immune system is one of our most intricate biological systems, maintaining bodily
homeostasis. Substances that modulate the immune responses are called immunomodulators.
In Unani system of medicine (USM), immunity is referred to as Quwate-Mudafia, which is part
of Quwa and is maintained by Tabiyat. In Unani literature, many single drugs and formulations
have been used for their immunomodulatory effect, apart from other therapeutic properties. Single
Unani drugs with established immunomodulatory activity are Gilo (Tinospora cordifolia),
Sibr (Aloe vera), Asgandh (Withania somnifera), Aamla (Emblica officinalis) etc. Compound
formulations are Jawarish Amla sada, Iksir-e-badan, etc. We are living in the age of evermutating
influenza viruses giving challenges to mankind (such as the recently prevailed SARSCoV-
2 pandemic); many Unani medicinal plants are still unexplored and need to be evaluated
for their immunomodulatory property, which can act as substitutes for conventional drugs. To
compile Unani immunomodulatory drugs under one heading, important critical texts of traditional
Unani medicine, indexed peer-reviewed journals, and authentic literature from online
sources were reviewed along with their reported in vivo/in vitro activities and clinical trials.
Unani drugs explored in this review are time-tested remedies and can be utilized in these rapidly
changing immunological challenges.