bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell surface properties of high‐ and low‐metastatic cell lines selected from a spontaneous mouse lung carcinoma
Authors: Steele John G. , Rowlatt Charles , Sandall Jane K. , Franks L. M. 
Published: February 2007

AbstractThe surface oligosaccharide residues, glycoproteins and sialyl components of CMT64 lung carcinoma cells and high‐metastatic sublines CMT167 and CMT181 have been studied in culture. (I) The total cellular sialic acid content did not differ appreciably between the three lines. However, the accessibility of surface sialyl groups, measured by metabolic incorporation of [3H]NAcmannosamine followed by neuraminidase hydrolysis, was decreased from 42% in CMT64 to 25% hydrolyzed in CMT181. (2) The major plasma membrane glycoproteins of the lines were radiolabelled by lactoperoxidase iodination, metabolic incorporation of [3H]fucose or labelling in the terminal sialyl residues by the NalO4‐NaB[3H]4 method and the labelled glycoproteins were analyzed by two‐dimensional gel electrophoresis. Each labelling technique identified a complex pattern of glycoproteins including a prominently labelled group of high‐molecular‐weight acidic sialoglycoproteins: GP200/4.9–5.1 (apparent molecular weight ×10−3/pI of iodoprotein); GP150/5.1–5.6; GP130/5.0–5.6; GP110/5.0; GP100/4.8 and GP100/5.0–5.4. (3) The neuraminidase‐susceptible glycoproteins on CMT64 and CMT181 were identified in the isoelectric focusing separation of the two‐dimensional gel separation by the charge difference caused by desialylation. The glycoproteins most susceptible to neuraminidase were the high‐molecular‐weight acidic glycoproteins which showed marked charge heterogeneity: GP150/5.1–5.6, GP130/5.0–5.6; GP100/5.0–5.4 and GP100/4.8. (4) Using these procedures we did not detect modifications between CMT181 and CMT64 and we conclude that the cultured cells of the sublines do not display marked surface glycoprotein alterations that reflect their enhanced spontaneous metastatic potential.
Cross‐linking of cell surface immunoglobulins induces epstein‐barr virus in burkitt lymphoma lines
Authors: Takada Kenzo 
Published: February 2007

AbstractAnti‐human immunoglobulin (Ig) antibodies with single‐chain specificities induced Epstein‐Barr virus (EBV) in various Burkitt lymphoma lines when the corresponding Ig chain was expressed on the cell surface. The F(ab')2 fragments of IgG antibody were as potent as intact Ig, while the Fab and Fc fragments gave no induction, indicating that cross‐linking of surface Ig was required for the induction. Simultaneously with EBV induction, anti‐Ig inhibited the uptake of 3H‐thymidine. This inhibition was also seen in EBV‐genome‐negative Burkitt lymphoma lines. In contrast, no effect on virus induction and cell growth was noted in lymphoblastoid lines of non‐neoplastic origin. The possible relationship between cell differentiation and latency of EBV‐carrier state is discussed.
Prospective study on the relationship between cervical neoplasia and herpes simplex type‐2 virus. I. Epidemiological characteristics
Authors: Vonka V. , Kaňka J. , Jelínek J. , Šubrt I. , Suchánek A. , Havránková A. , Váchal M. , Hirsch I. , Domorázková E. , Závadová H. , Richterová V. , Náprstková J. , Dvořáková V. , Svoboda B. 
Published: February 2007

AbstractTo determine the risk associated with previous herpes simplex type‐2 (HSV‐2) infection and possibly other virus infections, a prospective study of cervical neoplasia in more than 10,000 women was performed in the 1975–1983 period. The subjects were selected at random from an alphabetical listing of eligible women living in one district of Prague. At enrolment colposcopy and cervical cytology were performed, a blood sample was taken and data regarding education, socio‐economic status, personal habits and sexual and reproduction‐associated attributes were otained from each woman. A total of 10,683 women were enrolled; a complete set of data was obtained in 10,389 were invited for further colposcopical and cytological investigations after 2 years and 4 years, the other women were followed at 3‐to 6‐monthly intervals. In women with highly significant findings, histological investigation was performed. The total of 150 cases of moderate to severe dysplasia (i.e. cervical intraepithelial neoplasia, grade II, CIN II), 83 cases of carcinoma in situ(CIN III) and 21 cases of invasive carcinoma (INCA) were detected. More than 60% of the patients were ill at enrolment, the other cases developed in subjects with originally slightly suspicious (27 CIN II, 17 CIN III, 3 INCA) or negative findings (30 CIN II, 12 CIN III, 3 INCA). Analysis of the data indicated significantly positive correlation of one or more of these clinical conditions with a number of sexual and reproduction‐related attributes of which early age at first intercourse was most consistent. Among the other attributes, the smoking habit was associated with the highest risk of developing the disease. A negative correlation of cervical neoplasia with several attributes was demonstrated; of these diathermoelectrocoagulation of the ectopic epithelium and transformation zone of cervix was the most important single protective factor. On the basis of these findings, control subjects were selected for serological studies.
Prospective study on the relationship between cervical neoplasia and herpes simplex type‐2 virus. II. Herpes simplex type‐2 antibody presence in sera taken at enrolment
Authors: Vonka V. , Kanaka J. , Hirsch I. , Závadová H. , Krčmář M. , Suchánková A. , Řezácová D. , Brouček J. , Press M. , Domorázková E. , Svoboda B. , Havránková A. , Jelinek J. 
Published: February 2007

AbstractSera obtained at enrolment in the study from patients suffering from moderate to severe dysplasia (cervical intraepithelial neoplasia grade II), carcinoma in situ (cervical intraepithelial neoplasia grade III) and invasive carcinoma, or developing any of these conditions in the course of the prospective study, and from control subjects, were examined for herpes simplex type‐2 (HSV‐2) antibody presence. The controls were matched with the patients by age, age at first intercourse, number of sexual partners, smoking habits and history of diathermoelectrocoagulation of the ectopic epithelium and transformation zone of cervix. Only those subjects were selected as controls who remained free of pathological colposcopical and cytological findings throughout the observation period, i.e. for at least 4 years after their serum sample was obtained. The microneutralization test (MNT) and type‐2‐specific solid‐phase radioimmunoassay (SPIRA) were used as serological tests. No difference in the prevalence of HSV‐2 antibody between the patients and controls was revealed by either test. Various combinations of the results from the two tests also failed to show any difference between patients and controls. Moreover, no significant differences were observed in the prevalence of HSV‐2 antibody between patients suffering from the various pathological conditions and those diagnosed at enrolment and later in the course of the study. These results do not provide any support for the hypothesis of the involvement of HSV‐2 in cervical neoplasia.
Immunological responses to a murine mammary adenocarcinoma. outgrowth of pulmonary occult metastases induced by immunosuppressive perturbations
Authors: Yamamura Yasuhiro , Braunschweiger Paul G. , Ramananda Madyastha K. , Proctor Julian W. 
Published: February 2007

AbstractIntravenously (i.v.) or subcutaneously (s.c.) injected T1699 tumor cell subpopulations (Ts, and TL1–1) were destroyed more rapidly in BALB/c nu/nu athymic mice than in syngeneic DBA/2J mice. The number of artificial pulmonary metastases of TL1–1 tumors was significantly lower, and the growth rate of s.c. Ts, and TL1–1 tumors was correspondingly slower in the nude mice. No macroscopic outgrowth of pulmonary metastases of Ts tumors was detected in either group of s.c. tumor‐bearers, even though the s.c. tumors progressed and killed the hosts. Unexpectedly, however, s.c. implantation in normal DBA/2J mice of lung homogenates not only from DBA/2J but also from s.c. Ts tumor‐bearing BALB/c nu/nu mice produced tumors, suggesting that significant numbers of clonogenic Ts cells were present in the lungs of animals without spontaneous outgrowth of pulmonary metastases. Perturbations of s.c. Ts tumor‐bearing DBA/2J or BALB/c nu/nu mice with immunosuppressive drugs or with anti‐mouse thymocyte serum, respectively, induced rapid outgrowth of pulmonary metastases.
Lymphoreticular cells isolated by centrifugal elutriation from a mammary adenocaricinoma. i. characterization of an in situ lymphocyte suppressor population by surface markers and functional reactivity
Authors: Buessow Scott C. , Paul Ronald D. , Miller Alan M. , Lopez Diana M. 
Published: February 2007

AbstractThe procedure of centrifugal elutriation was evaluated as a means of purifying large numbers of in situ lymphocytes from enzymatically disaggregated mouse mammary tumors. The eluate obtained at a flow rate of 3.0 ml/min was optimal for high levels of lymphocyte recovery with low levels of contaminating tumor cells, polymorphonuclear leukocytes, and macrophages. The majority of the tumor infiltrating lymphocytes (90%) expressed the Thy 1.2 antigen, while less than 5% possessed surface immunoglobulin. Further analysis of the T lymphocyte population was accomplished by flow cytometric analysis of in situ lymphocytes stained with fluorescein‐conjugated monoclonal anti‐Lyt 2 antibodies. The results of such studies reveal an increase in the levels of Lyt 2+ lymphocytes within the in situ population. To determine whether these Lyt 2+ cells were functionally active as suppressor cells, the ISL1 were mixed with spleen cells from tumor bearers and then tested for their ability to respond to mitogen and TAA‐induced blast transformation of tumor‐bearer spleen cells. Removal of macrophages from ISL by Sephadex G‐10 columns did not alter the suppression. Treatment with monoclonal anti‐Lyt 1 antibody and complement did not affect the inhibition observed. However, treatment of ISL with anti‐Lyt 2+ monoclonal antibody and complement resulted in the elimination of the suppressor cell activity. We concluded that within the tumor‐infiltrating lymphoreticular cells there is a population of Thy 1.2+ Lyt 2.2+ lymphocytes responsible for the suppression of mitogen and tumor‐antigen‐induced blastogenesis.
Effect of hydrocortisone on the macrophage content, growth and metastasis of transplanted murine tumors
Authors: Acero Raffaella , Polentarutti Nadia , Bottazzi Barbara , Alberti Saverio , Ricci Maria Rosaria , Bizzi Adalgisa , Mantovani Alberto 
Published: February 2007

AbstractHydrocortisone (HC) reduced the macrophage content of four murine tumors to less than half of control values. Since HC causes monocytopenia and inhibits the recruitment of mononuclear phagocytes at sites of inflammation but does not affect the viability and proliferative capacity of macrophages, this finding suggests that the maintenance of macrophage levels in growing tumors is in part dependent upon the entry into the neoplasm of circulating monocytes. HC inhibited the growth of these tumors. The effect of HC was most marked on the mFS6 and MN/MCA1 sarcomas and 3LL carcinoma, with only marginal inhibition in the M109 carcinoma. HC augmented spontaneous and/or artificial (i.v.) metastasis in these tumor models. Cells from metastatic foci of the mFS6 sarcoma and M109 carcinoma inoculated i.m. were as susceptible to HC as cells from primary tumors. The effects of HC on macrophage content, growth and metastasis of the M109 carcinoma and mFS6 sarcoma were similar in mice with defective T‐cell function (nude or thymectomized) or defective NK activity (beige or antiasialo GM1‐treated) and in controls. The in vitro growth of the mFS6 and MN/MCA1 sarcomas was not modified by HC. Some inhibition of tumor‐cell proliferative capacity was observed with the 3LL and M109 tumors. Tumor cell had high‐affinity binding sites for glucocorticoid hormones, but these were not correlated to susceptibility in vivo. Thus it is unlikely that a direct interaction of HC with tumor cells accounts for the inhibition of tumor growth observed in vivo. To evaluate whether HC affected tumor growth by reducing the macrophage content of tumors, mFS6 sarcoma cells were transplanted, mixed with peritoneal macrophages into normal or HC‐treated mice. Macrophages did not affect the growth of tumor cells in normal mice, whereas in HC‐created animals lesions from mixtures of macrophages and sarcoma cells appeared earlier and weighed more than those from tumor cells alone or tumor cells and thymocytes. However, macrophages did not reconstitute growth of the other sarcoma (MN/MCA1) in HC‐treated mice. These results are consistent with the hypothesis that HC inhibited growth of some poorly immunogenic transplanted murine tumors, at least in part, by interfering with the macrophage levels in neoplastic tissues, tumor‐associated macrophages providing the conditions for optimal tumor‐cell proliferation at least in some neoplasms.
Induction of plasma membrane alterations in AKR‐2B mouse embryo fibroblasts by endogenous growth factors from malignant human cells
Authors: Marks Michael E. , Brattain Michael G. 
Published: February 2007

AbstractAKR‐2B mouse fibroblasts were treated with 50 μg/ml of crude transforming growth factor (TGF) of human origin. Cell surface proteins of treated cells were radioiodinated and compared to untreated cells at various times after the addition of TGF. Treated cells showed a several‐fold increase (0˜6‐fold) in cell surface 125I incorporation relative to normal cells at 24 h. Electrophoretic comparisons of treated and untreated cells showed large increases in the labelling of cell surface proteins of mol. wt. 50,000–90,000 from TGF‐exposed cells between 10 and 24 h post treatment. By 48 h post treatment, the electrophoretic profiles of TGF‐exposed cells had returned to a pattern similar to that of untreated cells. However, even after a 48 h exposure to TGF, the cells retained a transformed morphology indicating that the electrophoretic alterations were not simply due to the morphological transformation induced by TGF. The electrophoretic pattern of TGF‐treated cells at 24 h post treatment was similar to that of AKR‐2B cells permanently transformed by treatment with methylcholanthrene, but was clearly distinct from that induced by treatment of normal AKR‐2B cells with epidermal growth factor (EGF). EGF induced an increase in a protein of mol. wt. 60,000 in the electrophoretic profile taken 24 h post treatment. As with TGF, the appearance of electrophoretic profiles of EGF‐treated cells returned to ‚normal’︁ by 48 h. Again, these alterations did not appear to be dependent upon morphological changes since EGF‐treated cells showed a morphological transformation similar to that of cells treated with TGF, and this was maintained throughout the 48 h experimental period.
In vitro antibody‐dependent cellular cytotoxicity against human T‐cell leukemia/lymphoma virus (HTLV)‐producing cells
Authors: Miyakoshi Hideo , Koide Hiroko , Aoki Tadao 
Published: February 2007

AbstractHuman T‐cell leukemia/lymphoma virus (HTLV)‐producing cells were lysed by peripheral mononuclear cells (PMNC) from both anti‐HTLV antibody‐positive and ‐negative healthy persons in the presence of natural humoral antibodies against HTLV‐related antigens (antibody‐dependent cellular cytotoxicity, ADCC). PMNC from patients with T‐cell leukemia, however, did not possess the positive reaction even in the presence of natural antibodies. In contrast, when the antibodies were absent, no cytotoxicity was caused by PMNC from any donors. Additionally, the complement present in the antibody‐positive plasma did not contribute to humoral antibody‐dependent cytotoxicity in the presence of plasma without PMNC. Therefore, ADCC against HTLV‐producing cells may correlate with the prophylaxis of HTLV‐induced malignancy in vivo.
Monoclonal antibody to human T‐cell leukemia virus P19 defines polypeptide antigen in human choriocarcinoma cells and syncytiotrophoblasts of first‐trimester placentas
Authors: Suni Jukka , Närvänen Ale , Wahlström Torsten , Lehtovirta Pentti , Vaheri Antti 
Published: February 2007

AbstractIn previous studies we detected retrovirus RD114 p30‐related antigen in human placental syncytiotrophoblasts and antibodies in cord blood sera. We now report that a monoclonal IgG antibody specific for the p19 protein of human T‐cell leukemia virus (HTLV) reacts with human syncytiotrophoblastic antigen. When used in the immunoperoxidase tissue‐staining procedure, the monoclonal antibody reacted with the syncytiotrophoblastic cells in sections of all early placentas (<15 weeks of gestation), blighted ova and benign (hydatidiform mole) or malignant placental tumors (destructive mole, choriocarcinoma). In all cases the reactivity was mainly confined to the cytoplasm of the cells with syncytiotrophoblastic morphology. Normal embryonal or adult tissues tested were negative. In immunoblotting of cultured choriocarcinoma cells, anti‐HTLV p19 detected a single polypeptide at mol.wt 28,000 from proteins separated by electrophoresis.
The prognostic significance of two epithelial membrane antigens expressed by human mammary carcinomas
Authors: Wilkinson M. J. S. , Howell A. , Harris M. , Taylor‐Papadimitriou J. , Swindell R. , Sellwood R. A. 
Published: February 2007

AbstractAs many patients with mammary carcinoma are now treated by conservative forms of surgery, there is a need for prognostic information obtainable from the primary tumour alone. One possible source is the antigenic profile of tumour cells. Using an indirect immunoperoxidase technique, we stained histological sections of the primary tumour from 175 patients with each of two monoclonal antibodies (HMFG‐1, HMFG‐2), raised against milk fat globule membrane antigens known to be preserved in formalin‐fixed tissues. Sections were assessed by light microscopy as to both the overall distribution of antigen expressed and its site in tumour cells. The findings were related to relapse‐free survival by life‐table analysis. The median duration of follow‐up was 36 months. Two patterns of staining with antibody HMFG‐1 gave information of prognostic significance but staining with HMFG‐2 was without significance. Complete absence of staining with HMFG‐1 in 13 patients was associated with an extremely poor prognosis and 10 (77%) of these patients developed metastases within 18 months of follow‐up (p<0.001). Extracellular staining (ECS) in 22 patients, however, was associated with a favourable prognosis. As assessed by a semi‐quantitative method, only one patient (5%) demonstrating a high level of ECS developed metastases (p<0,004). These two patterns were analysed for a relationship to other prognostic indicators. Absence of staining was independent of histological grade, tumour size, axillary lymph node status and menopausal status. ECS was associated with low histological grade although this relationship was not absolute. In addition to their use in diagnosis, we conclude that monoclonal antibodies such as HMFG‐1 may be useful as prognostic indicators.
Increased incidence of mouse mammary tumor virus‐related antigen in tunisian patients with breast cancer
Authors: Levine P. H. , Mesa‐Tejada R. , Keydar I. , Tabbane F. , Spiefelman S. , Mourali N. 
Published: February 2007

AbstractBiopsies obtained from 74 Tunisian women with breast cancer (33 cases), benign breast disease (17 cases), and cervical cancer (24 cases) were assayed for the presence of an antigen cross‐reacting with gp52 of the mouse mammary tumor virus (MMTV) in order to determine the frequency and possible prognostic significance of this antigen in a form of rapidly progressing breast cancer designated poussée évolutive or PEV. Antigen was detected in 23/33 breast carcinomas (70%) but in none of the 41 control specimens. An evaluation of reactivity according to tumor aggressiveness and survival could be performed in retrospect on 29 of the breast cancer patients with a follow‐up of up to 11 years. The frequency of gp52‐related antigen was similar in the patients with the most aggressive form of PEV with inflammatory signs (8/12 or 67% positive) and those breast cancer patients without PEV (12/17 or 71% positive). Within each of the two groups, PEV+and PEV O, no correlation was observed between the presence or absence of antigen and the disease‐free interval or survival. We conclude that the identification of gp52‐related antigens in the breast cancer biopsies from North African women has implications different from these observed in other populations. While thus far not indicative of disease aggressiveness and prognosis, the higher frequency of detectable antigen in comparison to biopsies obtained from patients born in the United States and Europe may have relevance to the etiology and pathogenesis of this disease.
Studies on chemical carcinogen enhancement of epstein‐barr virus induced transformation of human neonatal and adult peripheral blood lymphocytes
Authors: Henderson Earl E. , Fronko Gerald 
Published: February 2007

AbstractThe effects of a wide range of selected chemical carcinogens on the frequency of Epstein‐Barr virus (EBV)‐induced transformation have been investigated. The carcinogens tested included direct‐acting chemicals and chemicals requiring either activation via reactions with nucleophiles, or cell‐mediated enzyme activation. Treatment with some but not all chemicals suspected of being carcinogens resulted in enhanced EBV‐induced transformation of neonatal or adult peripheral blood lymphocytes (PBLs). The temporal relationship between carcinogen exposure and EBV infection could dramatically influence the results of the chemical carcinogen‐cellular interaction as measured by the cells' ability to subsequently undergo morphologic transformation. This relationship was particularly evident when cells were treated with alkylating agents such as dimethylsulfonate (DMS) or N‐methyl‐N′‐nitro‐N‐nitro‐soguanidine (MNNG). Beginning at 24h, and at later times following EBV‐infection, cellular transformation became more resistant to the cytotoxic effects of DMS and, in contrast, more sensitive to the cytotoxic effects of MNNG. These diametrically opposed results clearly demonstrate the ability of EBV infection to alter the response of lymphocytes to chemical carcinogens as measured by transformation. The lymphoblastoid cell lines (LCLs) established from carcinogen‐treated PBLs had increased cloning efficiency. Furthermore, using radiolabelled, molecularly cloned subgenomic fragments of EBV DNA and DNA‐DNA hybridization, we have been able to detect an increased number of EBV genome equivalents in whole‐cell and high‐molecular‐weight cellular DNA extracted from LCLs established from MNNG as well as DMS‐treated PBLs. We propose that carcinogen enhancement of EBV‐induced transformation is an example of a two‐step mechanism of oncogenic transformation in primary human lymphoid cells. The possible significance of these findings in relation to potential development of lymphomas following EBV exposure will be discussed.