bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mn3O4 Tetrahedral with Carbonyldiimidazole Nanoflower Deposition on Laser‐Scribed Graphene for Selective Bio‐Capture
Authors: Remesh Sivainesh Devi , Chelvaraj Jeysree , Perumal Veeradasan , Gopinath Subash C. B. , Ovinis Mark , Karupannan Saravanan , Arumugam Natarajan , Kumar Raju Suresh 
Published: February 2025

ABSTRACTDengue fever, a mosquito‐borne viral infection, poses a significant global health challenge, particularly in tropical and subtropical regions. The absence of non‐effective vaccines and specific treatments underscores the need for advanced diagnostic tools for early detection and management. This study presents a novel biosensor for detecting dengue virus type 4 (DENV‐4) by combining carbonyldiimidazole nanoflower (CDI‐NF) with Mn3O4 on laser‐scribed graphene (LSG). Material characterization techniques, including Raman spectroscopy, TEM, XRD, XPS, and FTIR, were employed to confirm the successful integration of Mn3O4 and CDI‐NF, resulting in a unique 3D flower‐like structure. In order to verify the sensing efficiency, a selective DNA sample captured on LSG/Mn3O4‐CDI‐NF was investigated for specifc binding with Aedes aegypti target DNA through selective hybridization and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1 fM, where the sensitivity was confirmed by large transfer resistance (Rct) before and after hybridization with a regression coefficient 0.97373. EIS results demonstrated successful surface modifications and the biosensor's specificity in distinguishing between complementary, mismatched, and non‐complementary target sequences. The biosensor's ability to differentiate between these sequences highlights its potential for accurate and targeted DENV‐4 detection, offering a promising avenue for advancing dengue diagnostics.
‘Where are the adults?’: Troubling child‐activism and children's political participation
Authors: Hunter Sharon , Cassidy Claire 
Published: February 2025

AbstractChildren's political participation is a well‐established theme in childhood studies. In this article we offer an original account of child activism that takes into account the entangled and emergent aspect of children as activists. We begin with a historical and a conceptual review, noting the importance of mid‐20th century developments such as May 1968 but tracing their roots to the earlier community‐derived activism of the US civil rights movement—a long episode of activism not normally included in accounts of children's political involvement. We consider the ways in which adults surround children, creating contexts and working for and through them with diverse political and social ends in mind. It has been a dominant focus of adult activity to facilitate children's participation rights. These rights are defined in international legislation as non‐contingent, that is, explicitly predicated on an absence of responsibility. Regardless, the relational aspect of responsibility is present in children's lives in families, churches, schools and wherever adults interact with children, whether to protect, instruct, direct or guide them. We conclude by indicating how current historical and conceptual frames do not allow for the complex understanding required to account for children's agency understood as necessarily entangled with adults’ prior being in the world. We advocate a radical relational turn in childhood studies to leave behind the lacuna that arise from individualist accounts of child‐activism.
Methods for Eluting Intact Extracellular Vesicles From Aptamer‐Based Affinity Chromatography: A Critical Evaluation Based on Downstream Applications
Authors: Miller Lian , Misra Manjusri , Li Huiyan 
Published: February 2025

ABSTRACTExtracellular vesicles (EVs) are nanosized vesicles released by cells, containing molecular cargo such as proteins and nucleic acids. EVs offer promising avenues for the detection of biomarkers of disease and are excellent candidates for drug delivery and therapeutics. Although EVs can be obtained from biological fluids, it is challenging to obtain intact EVs from complex fluids and there is no universally accepted standard method of isolating EVs. When affinity chromatography‐based isolation is used to isolate EVs from complex biofluids, there exist multiple ways to elute intact EVs from capture. This review aims to identify effective EV elution methods for preserving EV integrity and bioactivity after capture on aptamer‐functionalized substrates, addressing the requirements of various downstream applications. We hypothesize that when used for elution, different materials and techniques influence the characteristics of EVs, such as their molecular content and bioactivity. The elution reagent and technique must be selected for the intended application for isolated EVs. However, currently, there is no agreement on the optimal elution method for EVs. This literature review aims to evaluate the different methods used to elute intact EVs from capture with regards to the downstream applications of isolated EVs. Based on the results of our analysis of recent literatures, the two elution reagents that are optimal for general purposes of the eluted intact EVs are deoxyribonuclease I and complementary oligonucleotides, as they both preserve EV characteristics that are required for molecular analysis and bioactivity, such as maintained morphology and protein profiles.
Mediation Effect of CSF Substance P on the Association Between Smoking and Sleep
Authors: Zhang Xie , Chen Lingling , Wu Yuyu , Chen Yu‐Hsin , Luo Xingguang , Xu Zeping , Hu Weiming , Kang Yimin , Chen Li , Liu Yanlong , Wang Fan , Liu Danhui 
Published: February 2025

AbstractBackgroundCigarette smoking has been linked to severe and persistent sleep disturbances alongside notable fluctuations in neuropeptide levels. Substance P (SubP), influenced by smoking, also impacts sleep‐wake cycles. However, its specific role in smoking‐induced sleep disorders remains unclear. This study aimed to explore the connection between cigarette smoking and sleep quality by examining SubP levels in cerebrospinal fluid (CSF) and identifying potential treatment avenues for sleep disorders.MethodsA total of 146 Chinese men (93 nonsmokers, 53 active smokers) undergoing lumbar puncture before anterior cruciate ligament reconstruction were enrolled. Clinical data and Pittsburgh Sleep Quality Index (PSQI) scores were assessed, followed by CSF sample collection and CSF SubP level measurement.ResultsActive smokers exhibited significantly higher PSQI scores (4.02 ± 2.27 vs. 2.60 ± 2.46, p < 0.001) and CSF SubP levels (2111 ± 212 vs. 1821 ± 289, p < 0.001) compared to nonsmokers. A negative correlation (r = −0.434, p < 0.001) between SubP levels and PSQI scores was observed in all participants and nonsmokers, while no correlation (r = −0.044, p = 0.72) was found in active smokers. Logistic regression analysis across different dimensions of sleep disorders indicated associations between CSF SubP levels and sleep quality as well as daytime dysfunction (OR = 0.439 (0.211–0.891), p = 0.025; OR = 0.308 (0.152–0.608), p = 0.001). Mediation analysis suggested that CSF SubP levels mediated the relationship between smoking and sleep.ConclusionCSF SubP levels are elevated in active smokers and appear to play a mediating role in the relationship between smoking and sleep regulation, as evidenced by a negative correlation between CSF SubP levels and PSQI scores.
A comprehensive review of hydrogel strategies for repairing peripheral nerve injuries
Authors: Jia Shicheng , Zhou Hongfa , Chen Jiayou , Lin Jiashan , Zhu Xinlei , Weng Jian , Li Wei , Yu Fei 
Published: February 2025

AbstractAs an etiological factor underlying physical and mental disability in humans, peripheral nerve injuries (PNIs) can induce pain, sensory impairment, and disability. Despite their regenerative ability, peripheral nerves cannot self‐repair after severe defects. While nerve grafting is the gold standard for the treatment of PNIs, it is limited by graft versus host reactions, surgical complications, and limited donor nerves. As the field of material science continues to develop, hydrogels have been proposed for use in PNI repair after their biomodification, targeted modification, or loading with biological factors and cells. This article reviewed research advances in hydrogels used for PNI repair, including simple hydrogels and composite functionalized hydrogels loaded with biological factors and cells. Based on the findings from these reviews, we determined that further clarification of the mechanisms of action for hydrogels and loaded biological factors in modulating cellular functions is necessary. In addition, there is a need to further explore the synergistic effect of novel functionalized hydrogels with other biological, physical, or biochemical factors. While clinical trials are still limited, scientific efforts are expected to promote the application of hydrogels in the field of PNI repair.
Circular Economy Transition in Bangladesh's Ready‐Made Garments Sector: Investigating the Key Barriers Through a Fuzzy DEMATEL Analysis
Authors: Salman Sheak , Shanta Mehedi Hasan , Uday Rifath Mahmud , Rahman Rafat 
Published: February 2025

ABSTRACTThis study focuses on Bangladesh's thriving ready‐made garments (RMG) sector, which plays a pivotal role in the nation's economy. Though the sector has achieved remarkable success in garment exports, transitioning from linear economy (LE) to circular economy (CE) practices is essential for its sustainable production and economic growth. This study identifies and analyzes the critical barriers to the adoption of CE practices in Bangladesh's RMG sector using two systematic stages. The first stage utilizes the identification of 12 key barriers by applying the Delphi method, and the subsequent stage utilizes the Fuzzy Decision‐Making Trial and Evaluation Laboratory (DEMATEL) method to explore the causal relationships among these barriers, shedding light on their significance and interdependencies. The findings of the study indicate that limited funding for circular business models emerges as the most influential barrier, underscoring the importance of ensuring adequate funds for CE implementation. Inadequate collaboration of entrepreneurs and researchers and lack of professional in environmental management further hinder the progress, highlighting the need for building collaboration for research and advancements and involving experts for environmental management. This research contributes to sustainable production practices in emerging economies and underscores the importance of CE principles in a global context. The study's comprehensive approach provides valuable insights for RMG industry stakeholders, aiming to enhance CE adoption in the RMG sector, thereby prioritizing the strategic efforts and promoting sustainable practices. By identifying and addressing these barriers, Bangladesh's RMG industry can advance its journey towards a more circular and environmentally responsible future.
Private vs. Public: Differential Impacts of Sustainable Innovation on ESG Performance in the Digitalize Era
Authors: Hongbin Ying , Fei Wu , Zhijie Lu , Cifuentes‐Faura Javier 
Published: February 2025

ABSTRACTThe environmental, social, and governance (ESG) rating evaluates a company's value practices and impacts its operations. This study examines the effect of corporate sustainable innovation on ESG performance (ESGP) using data from 2009 to 2021 from Chinese A‐share listed companies. The analysis's findings, which utilize panel data models, indicate that effective, sustainable innovation enhances corporate ESGP. Moreover, the study demonstrates that private enterprises experience a more pronounced improvement in ESGP through sustainable innovation than public enterprises. Additionally, digital transformation positively moderates sustainable innovation and the ESGP relationship. These findings emphasize the financial advantages of sustainable innovation for business enterprises and the need for investor and governmental attention. Furthermore, prioritizing continuous innovation and advancing digital transformation efforts can further strengthen the constructive impact of sustainable innovation on ESGP.
Development and Validation of Prognostic Characteristics Associated With Chromatin Remodeling‐Related Genes in Ovarian Cancer
Authors: Chen Guansheng , Li Wenjing , Guo Jiayi , Liu Lingyu , Wang Yongjun 
Published: February 2025

ABSTRACTBackgroundOvarian cancer (OC) is a prevalent malignant tumor in the field of gynecology, exhibiting the third highest incidence rate and the highest mortality rate among gynecological tumors. Chromatin remodeling accomplishes specific chromatin condensation at distinct genomic loci and plays an essential role in epigenetic regulation associated with various processes related to cancer development.MethodsDifferentially expressed genes (DEGs) between OC and control samples were screened from The Cancer Genome Atlas (TCGA) and Genotype‐Tissue Expression (GTEx) databases, combined with chromatin remodeling‐related genes (CRRGs) obtained from the GeneCards database to identify differentially expressed CRRGs (DECRRGs). Enrichment analysis and protein–protein interaction (PPI) network were performed on the DECRRGs. Prognostic genes of OC were screened using univariate Cox and least absolute shrinkage and selection operator (Lasso) analyses. A risk model based on prognostic genes was developed, and the survival probability of OC patients in different risk groups was analyzed by Kaplan–Meier (KM) curve. Finally, the expression levels of prognostic genes were validated by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting.ResultsIn total, 7 potential prognostic genes associated with the progression of OC patients were obtained, including ARID1B, ATRX, CHRAC1, HDAC1, INO80, MBD2, and SS18. Based on the expression level of prognostic genes, OC patients were divided into high‐risk group and low‐risk group. Survival analysis indicated that patients classified into the high‐risk group had higher mortality rates, which enables this prediction model to be utilized as an independent predictor of OC. Immunocorrelation analysis showed that low‐risk patients were more likely to benefit from immunotherapy.ConclusionIn this study, we have identified 7 prognostic genes, including ARID1B, ATRX, CHRAC1, HDAC1, INO80, MBD2, and SS18. Overall, our findings provided a foundation for further comprehension of the potential molecular mechanisms underlying OC pathogenesis and progression.
Student Psychotherapists' Experiences of Self‐Empathy: An Interpretative Poetic Inquiry
Authors: Jack Kirsten , Aithal Supritha 
Published: February 2025

ABSTRACTBackgroundInterpersonal empathy is a well‐researched concept in the counselling and psychotherapy literature, although less is known about how it is experienced at an intrapersonal level. What is known is that self‐empathy involves a change in perspective and a re‐evaluation of events, in kinder and less judgemental ways.AimThe aim of this study was to understand and appreciate the meaning of self‐empathy to trainee creative psychotherapists. The research was in the context of the students' own self‐care and intrapersonal relating.Methodology and MethodsAn interpretative poetic inquiry design was utilised. Interviews were conducted with 4 trainee creative psychotherapists at a university in the Northwest of England, UK.ResultsSelf‐empathy was experienced as a process, often involving several aspects of the self, to understand and accept feelings and events in the context of self‐care. Development of self‐empathy involved internal dialoguing through activities such as journaling, poetry writing, meditation, and being outdoors. The use of interpretative poetic inquiry enabled the emotional aspects of the data to be distilled and combined reflexively with the experiences of the researchers.ConclusionsThis research provides contemporary insights into the experiences of self‐empathy from the perspective of a small number of trainee creative psychotherapists. It has implications for students in terms of self‐understanding and self‐care, and for educators, to inform future psychotherapist preparation and curriculum development. Finally, there are implications for future research using interpretative poetic inquiry as a reflexive approach, which embraces the presence of the researcher in the research.
Glucose Oxidase Promoting Cellulase‐Assisted Hydrodistillation for the Extraction of Essential Oil From Eleutherococcus Senticosus
Authors: Zhao Xu , Chen Zeshao , Xi Gaolei , Zhao Shengchen , Cao Ke , Wang Qiyue , Zhang Yinghao , Ma Shengtao , An Nan , Yu Shuai , Jia Tao 
Published: February 2025

ABSTRACTEleutherococcus senticosus essential oil (ESEO) has the function of clearing heat and detoxifying, delaying aging. The ESEO yield obtained by traditional extraction methods is low because essential oils are encased in plant cell structures and are difficult to extract. In this work, we adopted the method of glucose oxidase promoting cellulase‐assisted hydrodistillation, and utilized the interaction of glucose oxidase and cellulase to promote the full destruction of cellulose in plant cells, resulting in a large amount of internal essential oil flowing out and improving the yield of ESEO. By optimizing the main factors of enzymatic hydrolysis, the optimal extraction conditions for ESEO were determined through Box–Behnken design experiments. The ratio of glucose oxidase to cellulase is 1:20, the concentration of the enzyme is 1.02%, the temperature of enzymatic hydrolysis is 40.0°C, and the pH value of enzymatic hydrolysis is 3.44. The optimal yield was 0.140%. The yield of essential oil extracted from E. senticosus was increased by 49.9% compared to a single cellulase‐assisted extraction method. The extraction process used in this work is milder, more environmentally friendly, and more efficient, which has important reference value for the industrial extraction and utilization of medicinal plants.
Comprehensive Characterization and Bioactivity Assessment of Titanium Dioxide Nanoparticles Synthesized Using Chrysanthemum indicum Leaf Extract
Authors: Din Maida Salah Ud , Naveed Muhammad , Aziz Tariq , Makhdoom Syeda Izma , Waseem Muhammad , Khan Ayaz Ali , Al‐harbi Mitub , Alasmari Abdullah F. 
Published: February 2025

ABSTRACTBionanotechnology is the synthesis of environmentally friendly, biosynthetic nanomaterials by combining nanotechnology and biotechnology. Chrysanthemum indicum leaf extract in aqueous medium was used in the presented research work to produce titanium dioxide (TiO2) nanoparticles using a dependable and environmentally friendly method. The TiO2 nanoparticles derived from C. indicum were examined using scanning electron microscopy (SEM) and Fourier‐transform infrared spectroscopy. The diesel degradation activity of the TiO2 was evaluated, showing a significant reduction in diesel concentration (8%) after a 10‐day incubation period. Furthermore, TiO2’s hemolytic, antidiabetic, anti‐inflammatory, and antioxidant properties were highlighted. Cubical‐shaped nanoparticles with an even distribution were found by SEM analysis. When compared to standard drugs, the biosynthesized TiO2 nanoparticles demonstrated hemolytic potential, anti‐inflammatory (albumin denaturation assay), antidiabetic, and antioxidant (2,2‐diphenyl‐1‐picrylhydrazyl assay) properties. These results imply that TiO2 nanoparticles function as advantageous biological agents and are crucial to the bioremediation process. Due to the great potential of TiO2 nanoparticles, the notable characteristics of biosynthesized TiO2 nanoparticles suggest their potential useful applications in the biodegradation field in the future.
Metastatic Clear Renal Cell Carcinoma in Urinary Bladder at Presentation—A Rare Incidental Case Report and Literature Review
Authors: Ali Rao Nouman , Anwaar Adeel , Irfan Wajiha , Hameed Farooq , Haq Inam Ul , Asif Raza Muhammad , Karamat Riyan Imtiaz , Akilimali Aymar 
Published: February 2025

ABSTRACTThis case highlights the rare occurrence of concurrent clear cell renal carcinoma with metastatic urinary bladder involvement, presenting as hematuria and flank pain. Initial screening revealed both renal and bladder tumors, confirmed as metastases. Histopathology emphasized thorough investigations for unexplained urinary symptoms and the need for multimodal treatment and follow‐up.
Design Strategies to Enhance Copper Electrocatalytic Performance for Nitrate‐to‐Ammonia Electroreduction
Authors: Lombardi Simone , Mostoni Silvia , Mirizzi Lorenzo , Scotti Roberto , Viscardi Rosanna , Muhyuddin Mohsin , D'Arienzo Massimiliano , Santoro Carlo 
Published: February 2025

AbstractElectrochemical nitrate reduction (NO3−RR) is being recognized as a sustainable approach to synthesizing ammonia which is essential for the chemical industry and a key agricultural input conventionally produced through the hard‐to‐abate Haber Bosch process. Among various transition metals, copper‐based electrocatalysts stand out in efficaciously carrying out this reaction owing to their superior electrocatalytic activity and selectivity. In this context, here, current state of research and advanced scientific understandings of employing Copper for NO₃−RR are succinctly, but comprehensively, presented while focusing on its design strategies to enhance the electrocatalytic performance. First, the NO3−RR reaction mechanisms taking place at the surface of copper are described, followed by a discussion of its unique attributes in facilitating ammonia electrosynthesis. Then, various electrocatalyst fabrication routes and designing strategies are reviewed, emphasizing the role of the evolved structure, morphology, textural properties and surface chemistries in improving the reaction kinetics. Nanostructuring, facet and defect engineering, support, doping, alloying, heterojunction and the role of single active catalysts (SACs) centers as the key parameters for enhanced electrocatalytic behavior are highlighted.
Facile Synthesis Toward Surface Decorated Ir1Ox@Pd35 with Low Iridium Content for Highly Efficient and Durable Proton Exchange Membrane Water Electrolyzer
Authors: Choi Hosung , Ji Choi Hee , Choi Hyuckjae , Na Geumbi , Lee Jongmin , Kim Shin‐Yeong , Kim Junha , Shin Yoojin , Cho Yong‐Hun , Sung Yung‐Eun 
Published: February 2025

AbstractHerein, we introduce an iridium‐decorated palladium nanocluster (Ir1Ox@Pd35) synthesized via an epoxide‐assisted sol‐gel method. The nanocluster had a unique surface‐decorated structure, as confirmed by transmission electron microscopy (TEM) and synchrotron‐based X‐ray photoelectron spectroscopy (XPS). Structural characterization indicated that the Ir shell inhibited Pd growth by forming a porous surface‐decorated structure, contributing to enhanced activity. With low Ir loading (0.10 mgIr cm−2), Ir1Ox@Pd35 exhibits superior oxygen evolution reaction activity and durability compared to commercial IrO2. Ir1Ox@Pd35 also exhibited high performance and durability in a proton exchange membrane water electrolyzer (PEMWE), including a 20‐fold increase in Ir utilization compared to IrO2‐based systems. Featuring tailored structures and enhanced Ir utilization, Ir1Ox@Pd35 offers a sustainable path for PEMWEs.