bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revisional endoscopic sleeve gastroplasty versus semaglutide and tirzepatide for weight recidivism after sleeve gastrectomy
Authors: Bahdi Firas , Shah Sagar , Dahoud Fadi , Farooq Maryam , Kozan Philip , Kim Stephen , Sedarat Alireza , Shen Na , Thaker Adarsh , Kolb Jennifer M. , Dutson Erik , Muthusamy V. Raman , Issa Danny 
Published: February 2025

SummaryBackground and AimsWeight recidivism following sleeve gastrectomy (SG) is common. Adjuvant treatments include new glucagon‐like peptide 1 and glucose‐dependent insulinotropic peptide receptor agonists (GLP1/GIP‐RA) or revisional endoscopic sleeve gastroplasty (R‐ESG). We here compare the outcomes of these treatments.MethodsA retrospective study of patients ≥18 years with prior SG treated with semaglutide, tirzepatide or R‐ESG for weight recidivism between January 2019 and 2023 at large academic centre. Primary outcomes were total body weight loss (TBWL) and adverse events (AEs). Secondary outcomes were changes in metabolic parameters. Tertiary outcome was to compare GLP1/GIP‐RA outcomes in SG patients to matched patients with intact stomach.ResultsOur study included 68 (prior SG + GLP1/GIP‐RA) and 22 (prior SG + R‐ESG). R‐ESG offered higher TBWL% than GLP1/GIP‐RA at 3 (11.2% vs. 4.3%, p < .001), 6 (13.5% vs. 6.8%, p < .001) and 12 months (13.4% vs. 9.2%, p = .07) with no significant difference in AEs or change in metabolic parameters. On subgroup analysis, tirzepatide achieved similar 12‐months TBWL% as R‐ESG (13.2% vs. 13.4%, p = .9) and significantly more than semaglutide (13.2% vs. 8.1%, p = .04). Compared to patients with intact stomach (n = 87), GLP1/GIP‐RA achieved significantly lower TBWL% in patients with prior SG at 3 (4.3% vs. 5.7%, p = .02), 6 (6.8% vs. 9.2%, p = .02) and 12 months (9.2% vs. 12.7%, p = .03). Medication refills were difficult in 41.3% of patients.ConclusionsIn a single‐centre real‐world experience study, R‐ESG and tirzepatide appear to offer more weight loss than semaglutide in SG patients with weight recidivism although GLP1/GIP‐RA were underdosed. GLP1/GIP‐RA achieved higher weight loss in patients with intact stomach than those with prior SG.
Effect of Precise Pulmonary Rehabilitation Nursing Intervention Combined With Simultaneous Inhalation Therapy and Noninvasive Ventilation for Chronic Obstructive Pulmonary Disease Patients With Chronic Hypercapnic Respiratory Failure
Authors: Xiao Jian , Wu Yan , Wang Limei , Zhou Hong 
Published: February 2025

ABSTRACTBackgroundChronic obstructive pulmonary disease (COPD) is a progressive heterogeneous pulmonary disorder with an increasing high prevalence, particularly in the older. The objective of this study is to assess the effect of noninvasive ventilation combined with simultaneous nebulization during precise pulmonary rehabilitation nursing intervention in COPD patients with chronic hypercapnic respiratory failure, compared to pulmonary rehabilitation nursing alone.MethodsIn total, 240 elderly COPD patients with chronic hypercapnic respiratory failure were randomized controlled into either a precise pulmonary rehabilitation nursing model group (control group) or noninvasive ventilation coupled with simultaneous nebulization during pulmonary rehabilitation nursing (intervention group). For our evaluation, we considered general information, pulmonary function, exercise endurance, mental health, blood gas, inflammation, COPD severity, life quality, and overall efficacy of subjects before and on the 5‐, 30‐, 90‐day intervention.ResultsThe total effective rates were 95% and 77.5% in the intervention and control group, respectively. In detail, the intervention group showed statistically significant improvements in lung function, exercise endurance, psychological health, blood gas inflammation, overall severity, and quality of life outcomes, especially on the 90‐day intervention (p < 0.001).ConclusionsThe complex COPD patients with chronic hypercapnic respiratory failure may confer more gains from precise pulmonary rehabilitation nursing intervention combined with simultaneous inhalation therapy and noninvasive ventilation.
Recipient Outcomes After Utilization of Kidneys From Deceased Donors With Diagnosed Glioblastoma Multiforme
Authors: Nasika Venkata Kanaka Naga Karthik , Subramani Vanjinathan , Sharma Ashish , Singh Sarbpreet , Sethi Jasmine , Paladugu SreeVani , Jain Kajal , Bansal Navdeep 
Published: February 2025

ABSTRACTBackgroundThe exact incidence of donor tumor transmission in patients with glioblastoma multiforme (GBM) is still unknown. These transplants are usually considered as a high‐risk endeavor due to fear of potential spread especially with medical intervention leading to breach in blood–brain barrier. This report describes successful outcomes after renal transplantation from two donors with World Health Organization (WHO) Grade IV primary GBM who had undergone surgical excision with ventriculoperitoneal shunt (VPS).MethodsBetween January 2019 and June 2024, case records of organ donors where the primary cause of death was GBM were screened from the departmental database. Renal transplant patients from donors with a histopathological diagnosis GBM WHO Grade IV were included in the study. The follow‐up of these recipients was recorded for tumor transmission, delayed graft function, patient and graft survival.ResultsOnly kidneys were retrieved from these donors. The donor's kidneys were transplanted to four consented recipients after taking informed consent explaining the risks. During the mean follow‐up of 3 years, all four patients were alive with good graft function. Nuclear scan at 3, 6 months, and yearly thereafter showed no abnormal uptake or no evidence of donor‐transmitted tumor transmission in any of these patients.ConclusionHigh‐grade GBM donors with no evidence of systemic spread may be considered for renal transplantation.
Efficacy of high‐intensity interval training in individuals with type 2 diabetes mellitus: An umbrella review of systematic reviews and meta‐analyses
Authors: Poon Eric Tsz‐Chun , Li Hong‐Yat , Kong Alice P. S. , Little Jonathan P. 
Published: February 2025

AbstractHigh‐intensity interval training (HIIT) has gained attention as a potentially effective alternative to traditional exercise modalities for individuals with type 2 diabetes mellitus (T2DM). Previous studies have evaluated this exercise strategy with various regimens, comparator groups and outcomes, limiting the generalisability of findings. We performed a novel umbrella review to generate an up‐to‐date synthesis of the available evidence regarding the effect of HIIT on glycaemic control and other clinically relevant cardiometabolic health outcomes in individuals with T2DM, as compared with traditional moderate‐intensity continuous training (MICT) and/or non‐exercise control (CON). This umbrella review followed the Preferred Reporting Items for Overviews of Reviews guideline. Seven databases were searched until August 2024. Systematic reviews with meta‐analyses comparing HIIT with MICT and/or CON were included. Literature search, data extraction and methodological quality assessment (A MeaSurement Tool to Assess systematic Reviews 2 [AMSTAR‐2]) were conducted independently by two reviewers. Ten systematic reviews with meta‐analyses, encompassing 76 primary studies and 2954 unique participants, met the inclusion criteria. The data indicated that HIIT significantly improves glycosylated haemoglobin and cardiorespiratory fitness compared with CON (weighted mean difference [WMD]: −0.83% to −0.39% and 3.35–6.38 mL/kg/min) and MICT (WMD: −0.37% to −0.07% and 1.68–4.12 mL/kg/min) in individuals with T2DM. HIIT is also effective in improving other glycaemic parameters, including fasting blood glucose, fasting blood insulin and HOMA‐IR. Improvement in body composition, lipid profiles and blood pressure has also been observed following HIIT. Most systematic reviews received moderate to low AMSTAR‐2 score. This umbrella review supports HIIT as an efficacious exercise strategy for improving glycaemic control and certain relevant cardiometabolic health outcomes in individuals with T2DM. Our findings offer a comprehensive basis that may potentially contribute to informing physical activity recommendations for incorporating HIIT into T2DM management strategies.
Medical Data Classification Using Genetic Programming: A Systematic Literature Review
Authors: Maurya Pratibha , Kushwaha Arati , Prakash Om 
Published: February 2025

ABSTRACTBackgroundMedical data classification has always been a growing area of research. While machine learning techniques have been successfully applied in this field, the vast amount of data generated and the complexity of applications necessitate more robust and powerful methods, especially in the absence of domain expertise. Genetic programming (GP) being a flexible evolutionary approach can autonomously craft efficient classification programs merely from example data and has thus gained significant attention across various classification domains.ContentThis article presents a literature survey on the application of genetic programming to medical data classification. Reported studies are evaluated based on the examination of datasets, classifier architecture, and achieved classification accuracy. Additionally, we also discuss the strengths and weaknesses of genetic programming with other algorithms, covering aspects like classification accuracy, computational efficiency, interpretability, and resource consumption. The limitations of existing GP techniques and future directions are also presented in this study.ConclusionThe study presented in this article indicates that GP‐based classifiers perform better than other classifiers in the medical domain. To the best of our knowledge, this article is the first of its kind which discusses the application of GP explicitly in medical data classification. Through this article, we aim to enlighten the readers on key concepts of GP and encourage them to build new classifiers by exploring the potential and limitations of genetic programming.
Contact and communication: ZO‐2 and the Hippo pathway
Authors: Thomas Miranda 
Published: February 2025

The PDZ domain‐containing protein ZO‐2 is defined as a tight junction (TJ) protein, but is also known to have a role in the maintenance of cellular apicobasal polarity and to function as a signalling molecule in several pathways, including the Hippo pathway. In this issue, Liu OX et al. [(2024) FEBS J, https://doi.org/10.1111/febs.17304] report how the multiple protein binding sites of ZO2 protein allow it to act as a scaffold to facilitate its signalling functions.
Idiopathic Mild Platelet Dysfunction: Baseline Characteristics and Clinical Courses
Authors: Wiwatsomwong Nitchkan , Jantasing Ratchaneekorn , Akkawat Benjaporn , Uapresert Noppacharn , Rojnuckarin Ponlapat 
Published: February 2025

ABSTRACTIntroductionThe causes of nonsyndromic platelet storage pool disease are still unclear, and whether they are of genetic or acquired origin remains to be defined. The study aimed to describe the characteristics and natural history of this disorder.MethodsThis mostly retrospective cohort enrolled adults presenting with bleeding from platelet dysfunction. Platelet glycoprotein defects, von Willebrand disease, syndromic inherited platelet disorders and known acquired platelet dysfunctions were excluded. Available patients were retested by lumiaggregometry (Chrono‐Log) over 1 year after the initial diagnosis.ResultsThere was a total of 56 patients; 91% female, with a median diagnostic age of 28 years (interquartile range [IQR]: 24.5–38.5). The subnormal responses to ADP, epinephrine, collagen, and arachidonate were found in 91%, 82%, 55%, and 34%, respectively. Nineteen patients had von Willebrand factor levels measured. Twenty‐three subjects underwent repeat tests. Twenty‐one of them were female (91%), with a median age and follow‐up time of 37 years (IQR: 28–55) and 6 years (IQR: 3–12), respectively. Median ISTH‐BAT bleeding scores at diagnosis and follow‐up were 5 (IQR: 3–8) and 1 (IQR: 0–2), respectively. The common abnormalities were reduced responses to ADP combined with other agonists (83%). Twelve (52%) and five (22%) showed complete and partial platelet function recovery, respectively. None of the partial and non‐recovery groups had a bleeding score over 4 at follow‐up.ConclusionsIdiopathic mild platelet dysfunction was female‐predominant and showed spontaneous symptom resolution after a long follow‐up. Platelet function recovery was observed in most cases. Exogenous factors triggering this condition remain to be identified.
Role of Atg3, Atg5 and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori
Authors: Goncu Ebru , Tinartas Esen Poyraz , Gunay Busra , Ordu Tugce , Turgay Izzetoglu Gamze 
Published: February 2025

AbstractAutophagy is a cellular mechanism that enhances cell survival in response to various stressors, including nutrient deprivation; however, it also plays a pivotal role in the regulation of programmed cell death. This study examined the effects of autophagy‐related genes Atg3, Atg5 and Atg12 on apoptosis and autophagy during the degeneration of the posterior silk gland in Bombyx mori, employing RNA interference techniques. Apoptosis‐specific markers and autophagic processes were evaluated in both control and treatment groups. The knockdown of all three genes resulted in a significant reduction in autophagy, modifications in the apoptosis process, aberrant expression of p53 and impaired lysosomal function. It was determined that Atg3 is involved in the regulation of intracellular mitochondrial homeostasis. Following the silencing of Atg5, evidence was obtained indicating the gene's role in regulating lysosomal pH. Notably, the loss of Atg3 and Atg5 was associated with an increase in apoptotic markers, whereas the silencing of Atg12 inhibited apoptosis. Elevated levels of the p53 transcription factor following gene silencing suggested a potential interaction between these genes and p53. Our findings further underscore the importance of autophagy‐mediated cell death, involving Atg3, Atg5 and Atg12, in the proper progression of degeneration in the posterior silk gland. A comprehensive understanding of the molecular mechanisms that mediate the interaction between apoptosis and autophagy is essential for elucidating their roles in both physiological and pathological contexts.
Optimization of a semiflexible demand‐responsive feeder bus system using variable neighborhood search
Authors: Sartori Vieira Fábio , Sörensen Kenneth , Vanstenwegen Pieter 
Published: February 2025

AbstractA demand‐responsive feeder system (DRFS) presents an alternative to traditional feeder bus systems (TFS) in areas with low demand. This paper introduces an optimization problem to support the planning and operation of a DRFS. A variable neighborhood search (VNS) is employed to optimize the DRFS, focusing on minimizing passengers' travel times. The performance of the VNS is compared with that obtained by solving the mathematical model using a commercial solver (CPLEX) across two networks: a small network for validation and a larger suburban network that simulates a TFS. The results indicate that the VNS is a viable and efficient alternative for optimizing DRFS operations, providing flexibility in route and departure time adjustments, and achieving significant reductions in passenger travel times. The results also demonstrate that the DRFS outperforms the TFS in low‐demand areas. On average, the VNS improves upon CPLEX results, obtained within one hour of calculation time for the small network, by 2.3%. Using the same resources, the DRFS reduces the average passenger travel time by 9.6% compared to the TFS.
The Rise (and Fall?) of European Water Shrews: Phylogeography and Species Distribution Models Uncover the Impact of Climate Change
Authors: Neves T. , Tapisso J. T. , Gabriel S. I. , Rychlik L. , Ramalhinho M. G. , Borda‐de‐Água L. , Mishta A. , Mathias M. L. 
Published: February 2025

ABSTRACTAimPredicting the effects of climate change on species distributions, while considering their genetic differentiation and evolutionary history is crucial to develop conservation measures. Here we aim to: assess the phylogenetic and phylogeographical relationships between the European Neomys species and respective lineages; infer the demographic history of all genetic lineages and estimate the potential impact of future climate change on their distribution and persistence of the genetic lineages.LocationEurope.Time PeriodThe demographic analysis ranges from 350,000 ago to the present. The future climate change scenarios modelling ranges from the present to 2100.Major Taxa StudiesThree species belonging to the genus Neomys.MethodsWe reconstructed the phylogenetic relationships between the species in Europe and unveiled their demographic history based on cytochrome b gene sequences while using presence data to model how their distribution might change under distinct climate change scenarios.ResultsOur results confirm the existence of a distinct N. milleri lineage in the Italian Peninsula, whose population size has been relatively constant over time. Except for this lineage, all species' populations have likely been increasing until at least 25,000 years ago. While our future projections are beneficial to N. milleri, relatively inconsequential to N. fodiens, but suggest the likely extinction of N. anomalus, endemic to the Iberian Peninsula and the Italian lineage of N. milleri.Main ConclusionsThe Mediterranean Peninsulas, acting as refugia from the extreme cold conditions during the Last Glacial Period, contributed to the genetic differentiation of N. anomalus and the Italian lineage of N. milleri. However, as climate warms, the Peninsulas will no longer be able to sustain these species/lineages. Given the Peninsulas' status as biodiversity hotspots, this vulnerability of their ecosystems to climate change should mobilise conservation efforts guided by phylogeographic approaches, allowing us to uncover putative lineages or endemisms before their potential extinction.
Progranulin Protects Against Osteoporosis by Regulating Osteoclast and Osteoblast Balance via TNFR Pathway
Authors: Wang Shaoyi , Zhang Hengyan , Zhu Yanbin , Zhou Xiaocong , Zhai Haoxin , He Qiting , Zhu Xuetao , Zhang Yuanqiang 
Published: February 2025

ABSTRACTOsteoporosis is a disease of bone metabolism caused by an imbalance between osteoclast‐mediated bone destruction and osteoblast‐mediated bone formation. Tumour necrosis factor α (TNFα) has been reported to promote osteoclast generation and inhibit osteoblast generation. Progranulin (PGRN), which has a strong anti‐inflammatory effect, interacts with tumour necrosis factor receptor (TNFR). Serum and bone tissues from patients with or without osteoporosis were collected to analyse the relationship between PGRN content and bone metabolic markers. The role of TNFα and PGRN in osteoclast differentiation was explored by using RAW 264.7 cells and BMMs. MC3T3‐E1 cells and BMSCs were used to observe the role of TNFα and PGRN in osteoblast differentiation. The PGRN content in the serum and bone tissues of osteoporosis patients was lower than that in the serum and bone tissues of nonosteoporosis patients. TNFα promoted osteoclast differentiation, while PGRN inhibited this effect by interacting with TNFR1. PGRN inhibited TNFα‐mediated attenuation of osteoblast differentiation by interacting with TNFR1. Moreover, PGRN alone promoted osteoblast differentiation by interacting with TNFR2. Our findings reveal that PGRN can effectively inhibit TNFα‐induced osteoporosis and has a certain osteogenic effect. This discovery might provide a potential target for osteoporosis treatment.
Prediction of Hepatocellular Carcinoma Prognosis and Immunotherapy Response Using Mitochondrial Dysregulation Features
Authors: Yu Jia , Wu Shengli , Gong Jinglong , Deng Wenbing , Xiao Zhongsheng , Wu LiangLiang , Long Hong 
Published: February 2025

ABSTRACTHepatocellular carcinoma (HCC) is a major contributor to cancer‐related deaths globally. Although there have been improvements in identifying treating the disease, patient outcomes are still unfavourable because of the significant variation in HCC. Mitochondrial‐related genes (MRGs) are crucial in tumour metabolism, cell death and immune response, emerging as potential therapeutic targets. We analysed 2030 MRGs using TCGA, GEO and HCCDB18 databases. Differentially expressed genes were identified using edgeR and limma, and enrichment analysis was performed via the clusterProfiler package. A prognostic model was built using machine learning algorithms and evaluated using LOOCV. Immune infiltration was assessed with CIBERSORT, EPIC, MCPCounter and TIMER algorithms, and drug sensitivity was analysed using the CTRP and PRISM datasets. MRG expression levels are significantly associated with worse outcomes in HCC patients outperformed conventional clinical indicators in immune response revealed that individuals at high risk exhibited weaker immune responses, characterised by reduced immune scores, and elevated levels of CD8+ T cells and macrophages. Notably, high‐risk patients also displayed heightened susceptibility to chemotherapy agents such as paclitaxel and irinotecan. Abnormal MRG expression serves as a significant biomarker for HCC prognosis. The developed model accurately predicts disease progression and can guide personalised treatment, especially for immune and chemotherapeutic therapies. Further validation with broader clinical samples is needed.
Standard Protocols for Characterising Primary and In Vitro‐Generated Human Hepatocytes
Authors: Heydari Zahra , Gramignoli Roberto , Piryaei Abbas , Zahmatkesh Ensieh , Pooyan Paria , Seydi Homeyra , Nussler Andreas , Szkolnicka Dagmara , Rashidi Hassan , Najimi Mustapha , Hay David C. , Vosough Massoud 
Published: February 2025

ABSTRACTHepatocyte‐like cells (HLCs) derived from pluripotent stem cells (PSCs) or direct reprogramming are an unlimited source of human hepatocytes for biomedical applications. HLCs are used to model human diseases, develop precise drugs and establish groundbreaking regenerative cell‐based therapies. Primary human hepatocytes are the gold standard for studying human liver biology and pathology. However, their widespread use is limited by their rapid dedifferentiation in vitro, reliance on transplant‐rejected donor organs, poor scalability and significant batch‐to‐batch variations. Therefore, high‐quality ‘off‐the‐shelf’ HLCs are needed to overcome those limitations. Basic stepwise differentiation protocols have been developed to generate HLCs from PSCs. To evaluate the quality of the in vitro generated products, HLCs have been phenotyped using various methods. This review discusses various biological assays and methods available for the robust evaluation of HLC quality, emphasising the importance of using 24‐h cultured primary human hepatocytes (PHHs) as a reference standard for comparison.