bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and field evaluation of a novel sugar bait device for controlling residential vector mosquitoes in Zhejiang Province, China
Authors: Wu Yuyan , Zhang Chuan , Feng Wei , Fu Sanjun , Dong Weibo , Wang Jinna , Liu Qinmei , Li Tianqi , Luo Mingyu , Gong Zhenyu 
Published: March 2024

BackgroundMosquito-borne diseases pose serious public health threats in Zhejiang Province, China, and vector control is believed to be the primary method for reducing transmission. Due to severe resistance problems, effective and sustainable methods without chemical insecticides are urgently required to control mosquito vectors. Attractive toxic sugar baits (ATSB) are newly developed methods to control mosquitoes in recent decades with the core element sugar bait, which was invented according to the sugar-feeding behavior of mosquitoes. In this study, we developed a Novel Sugar Bait Device (NSBD) trap by combining sugar bait and physical adhesive capture technology. The study aimed to evaluate the effect of the NSBD trap on controlling mosquitoes in residential environments and to identify the optimal sugar solution concentration in the sugar bait of the NSBD for real use.MethodsFour residential villages in Ningbo City with similar geographic environments and mosquito densities were selected for field trials in 2022. One village (site 1) was designated as the control group, and three villages (sites 2–4) served as the test groups to assess the effectiveness of NSBD traps with different sugar solution concentrations (6, 8, and 10%) in the sugar bait. Larval and adult mosquito densities were monitored monthly before and semi-monthly after the trials using the CDC light trap and larval pipette method.ResultsBefore the trials, we monitored mosquito density for 3 months to confirm the baseline mosquito density among the four sites, and no statistical differences in adult and larval mosquitoes were found (adult, F = 3.047, p &gt; 0.05; larvae, F = 0.436, p &gt; 0.05). After the trials, all NCBD traps effectively controlled larval and adult mosquito densities, with the highest standard decrease rates of larval and adult mosquito densities at 57.80 and 86.31%, respectively, observed in site 4. The most suitable sugar solution concentration in the sugar bait was 10%.ConclusionNSBD traps effectively controlled mosquitoes in residential environments during field trials. Without the use of insecticides, this may be a promising choice for mosquito vector control to prevent mosquito-borne diseases.
Finite-Time H∞ Controllers Design for Stochastic Time-Delay Markovian Jump Systems with Partly Unknown Transition Probabilities
Authors: Guo Xinye , Li Yan , Liu Xikui 
Published: March 2024

This paper concentrates on the finite-time H∞ control problem for a type of stochastic discrete-time Markovian jump systems, characterized by time-delay and partly unknown transition probabilities. Initially, a stochastic finite-time (SFT) H∞ state feedback controller and an SFT H∞ observer-based state feedback controller are constructed to realize the closed-loop control of systems. Then, based on the Lyapunov–Krasovskii functional (LKF) method, some sufficient conditions are established to guarantee that closed-loop systems (CLSs) satisfy SFT boundedness and SFT H∞ boundedness. Furthermore, the controller gains are obtained with the use of the linear matrix inequality (LMI) approach. In the end, numerical examples reveal the reasonableness and effectiveness of the proposed designing schemes.
Reducing Immunoreactivity of Gluten Peptides by Probiotic Lactic Acid Bacteria for Dietary Management of Gluten-Related Diseases
Authors: Leszczyńska Joanna , Szczepankowska Agnieszka K. , Majak Iwona , Mańkowska Dorota , Smolińska Beata , Ścieszka Sylwia , Diowksz Anna , Cukrowska Bożena , Aleksandrzak-Piekarczyk Tamara 
Published: March 2024

Immunoreactive gluten peptides that are not digested by peptidases produced by humans can trigger celiac disease, allergy and non-celiac gluten hypersensitivity. The aim of this study was to evaluate the ability of selected probiotic strains to hydrolyze immunoreactive gliadin peptides and to identify peptidase-encoding genes in the genomes of the most efficient strains. Residual gliadin immunoreactivity was measured after one- or two-step hydrolysis using commercial enzymes and bacterial peptidase preparations by G12 and R5 immunoenzymatic assays. Peptidase preparations from Lacticaseibacillus casei LC130, Lacticaseibacillus paracasei LPC100 and Streptococcus thermophilus ST250 strains significantly reduced the immunoreactivity of gliadin peptides, including 33-mer, and this effect was markedly higher when a mixture of these strains was used. In silico genome analyses of L. casei LC130 and L. paracasei LPC100 revealed the presence of genes encoding peptidases with the potential to hydrolyze bonds in proline-rich peptides. This suggests that L. casei LC130, L. paracasei LPC100 and S. thermophilus ST250, especially when used as a mixture, have the ability to hydrolyze immunoreactive gliadin peptides and could be administered to patients on a restricted gluten-free diet to help treat gluten-related diseases.
Evaluation of a Commercial Serum Competitive Enzyme-Linked Immunosorbent Assay for Detection of Neospora caninum-Specific Antibodies in Raw Milk of Ruminants
Authors: Fereig Ragab M. , Altwaim Sarah A. , Frey Caroline F. 
Published: March 2024

Bovine neosporosis is an infection caused by the protozoan parasite Neospora caninum and has substantial veterinary hazards. Neosporosis cannot be controlled by vaccination or chemotherapy. Thus, accurate diagnosis followed by isolation and culling of infected animals is regarded as the most efficient method of control. In vivo diagnosis often relies on serologic testing of the animals, and milk represents a non-invasive and easy-to-collect sample matrix. However, indirect enzyme-linked immunosorbent assay (ELISA) specifically designed for antibody detection in milk are sometimes not easily available and it is tempting to use ELISA kits that are originally designed for use in serum in milk samples instead. Herein, we evaluated a widely used commercial ELISA (ID Screen® Neospora caninum competition Multispecies ELISA (ID. Vet, Grabels, France)), developed for detection of N. caninum antibodies in serum samples, for its performance on milk samples. Milk samples from dairy ruminants (cows, buffaloes, sheep, and goats; n = 149) were tested in parallel with the serum ELISA and a commercial milk ELISA as a standard test (Neospora caninum Milk Competitive ELISA, ID. Vet, Grabels, France). The detected prevalence values were 28.2% (42/149), 17.4% (26/149), and 17.4% (26/149) using milk ELISA, serum ELISA, and both ELISAs, respectively. Sensitivity, specificity, positive predictive value, and negative predictive value for the serum ELISA used with milk samples were 61.9%, 100%, 100%, and 87%, respectively. The agreement and kappa value between the two ELISAs were 89.3% and 0.70, respectively, suggesting substantial agreement. High values of Pearson correlation coefficient (0.904, p ≥ 0.0001) and area under the receiver operating characteristic (ROC) curve (0.789, p ≥ 0.0001) demonstrated the high diagnostic performance of the serum ELISA in milk samples. Also, a Bland–Altman Plot and histogram describing the frequency of distribution of ELISA optical densities confirmed the high agreement of both serum and milk ELISAs. The current results revealed the high specificity but moderate sensitivity of the serum ELISA used for milk samples compared with the milk ELISA. However, the excellent positive predictive value of the serum ELISA makes it an alternative option in case of the unavailability of milk ELISAs. With this study, we provided additional evidence that a widely used serum ELISA test kit may also be used for the detection of N. caninum antibodies in milk samples.
Artemisia spp. Essential Oils: From Their Ethnobotanical Use to Unraveling the Microbiota Modulation Potential
Authors: Polito Flavio , Di Mercurio Mattia , Rizzo Silvia , Di Vito Maura , Sanguinetti Maurizio , Urbani Andrea , Bugli Francesca , De Feo Vincenzo 
Published: March 2024

Background. The 2015 Nobel Prize in Medicine, awarded for the discovery of artemisinin in Artemisia annua, reignited interest in aromatic plants, including Artemisia absinthium L. This article delves into the historical, ethnopharmacological and medicinal significance of A. absinthium, examining its bitter taste noted since ancient Greek times and its association with medicinal properties throughout history. Despite being banned in the 20th century due to perceived health risks; recent research has led to the reconsideration of A. absinthium’s potential applications. This study focuses on the prebiotic efficacy of essential oils (EOs) from two Artemisia species: A. absinthium and A. annua. Materials and methods. A broth microdilution test, growth curve test and in vivo models were used to study the impact of low doses (from 0.5% v/v to 0.00048 v/v) of Artemisia spp-EO on the three probiotic strains (Lactobacillus, Lactobacillus casei and Saccharomyces boulardii). Results. These essential oils, when used in minimal concentrations (lower than 0.06% v/v), are safe and exhibit prebiotic effects on major probiotic strains, supporting the traditional culinary use of Artemisia spp. Conclusion. This research opens avenues for potential applications in the food industry, emphasizing the need for further exploration into the prebiotic properties of Artemisia spp-EOs and their influence on the microbiota.
Three-Dimensional Cultured Human Dermal Papilla Cells in HGC-Coated Environments Enhance Hair Regeneration and Artificial Skin Integration
Authors: Truong Thuy Trang , Park Heejin , Park Kyoung Hwan , Song Jin Jung , Lee Byoung-Seok , Huh Kang Moo , Kang Sun-Woong 
Published: March 2024

The rapid development of biomimetic materials in the field of regenerative medicine has made it possible to mimic natural cellular environments and allow in vitro systems to replace in vivo systems. In particular, the emergence of hexanoyl glycol chitosan (HGC) is playing an important role the development of 3-dimensional (3D) cell culture and tissue engineering. We employed HGC-coated dishes to cultivate human hair follicle dermal papilla (HDP) cells in 3D spheroids, assessing their ability to form hair-like structures. The study also tested the effect of minoxidil on these spheroids and explored their integration into artificial skin models. HDP cell spheroids successfully formed hair-like structures within the 3D culture. Minoxidil treatment showed enhanced hair growth in spheroids cocultured with keratinocytes. In addition, transplantation of these spheroids into artificial skin led to the formation of functional papilla structures, suggesting a closer mimicry of human skin. Hair-like structure formation and successful integration into artificial skin models pave the way for innovative approaches in hair loss treatment research, cosmetic, and pharmaceutical evaluations and skin restoration therapies.
Engineered Basic Fibroblast Growth Factor Specifically Bonded with Injectable Extracellular Matrix Hydrogel for the Functional Restoration of Cerebral Ischemia in Rats
Authors: Shi Chunying , Liu Qi , Sun Feng , Zhang Guangyu , Deng Mingru , Xu Bo , Yuan Haicheng 
Published: March 2024

Cerebral ischemia was one of the leading causes of mortality and disability worldwide. Extracellular matrix (ECM) hydrogel held great potential to replace volumetric brain tissue loss following ischemic injury but with limited regenerative effect for functional restoration when implanted alone. In the present study, an engineered basic fibroblast growth factor (EBP-bFGF) was constructed, which fused a specific ECM-binding peptide (EBP peptide) with bFGF. The recombinant EBP-bFGF showed typical binding capacity with ECM without affecting the bioactivity of bFGF both in vitro and in vivo. Furthermore, the EBP-bFGF was used for bioactive modification of ECM hydrogel to repair cerebral ischemia. The combination of EBP-bFGF and ECM hydrogels could realize the sustained release of bFGF in the ischemic brain and improve the regenerative effect of ECM, which protected the survival of neurons, enhanced angiogenesis, and decreased the permeability of blood–brain barrier, ultimately promoted the recovery of motor function. In addition, transcriptome analysis revealed neuregulin-1/AKT pathway involved in this process. Therefore, EBP-bFGF/ECM hydrogel would be a promising therapeutic strategy for cerebral ischemia.
A Standardized Rat Model to Study Peri-implantitis of Transmucosal Osseointegrated Implants
Authors: Liu Xingchen , Deng Shudan , Li Xiyan , Liu Haiwen , Li Zhixin , Wu You , Luo Pu , Zhong Xinyi , Huang Ruoxuan , Liu Runheng , Wu Xiayi , Huang Baoxin , Chen Zetao , Chen Zhuofan , Chen Shoucheng 
Published: March 2024

With the high incidence rate, distinctive implant characteristic and unique infection pattern, peri-implantitis (PI) requires a specially designed implant animal model for the researches on the pathogenesis and treatments. Previous small-animal PI models exhibit variability in implant site selection, design, and surgical procedures resulting in unnecessary tissue damage and less effectivity. Herein, a quantitative-analysis-based standardized rat model for transmucosal PI-related research was proposed. After dissecting the anatomic structures of the rat maxilla, we determined that placing the implant anterior to the molars in the rat maxilla streamlined the experimental period and enhanced animal welfare. We standardized the model by controlling the rat strain, gender, and size. The customized implant and a series of matched surgical instruments were appropriately designed. A clear, step-by-step surgical process was established. These designs ensured the success rate, stability, and replicability of the model. Each validation method confirmed the successful construction of the model. This study proposed a quantitative-analysis-based standardized transmucosal PI rat model with improved animal welfare and reliable procedures. This model could provide efficient in vivo insights to study the pathogenesis and treatments of PI and preliminary screening data for further large-animal and clinical trials.
Accurately Controlled Tumor Temperature with Silica-Coated Gold Nanorods for Optimal Immune Checkpoint Blockade Therapy
Authors: Yun Wan Su , Yang Wonseok , Shim Man Kyu , Song Sukyung , Choi Jiwoong , Kim Jeongrae , Kim Jinseong , Moon Yujeong , Jo SeongHoon , Lim Dong-Kwon , Kim Kwangmeyung 
Published: March 2024

Photothermal therapy (PTT) at mild temperatures ranging from 44 to 45 °C holds tremendous promise as a strategy for inducing potent immunogenic cell death (ICD) within tumor tissues, which can reverse the immunosuppressive tumor microenvironment (ITM) into an immune-responsive milieu. However, accurately and precisely controlling the tumor temperature remains a formidable challenge. Here, we report the precision photothermal immunotherapy by using silica-coated gold nanorods (AuNR@SiO
2
), and investigating the optimal administration routes and treatment protocols, which enabled to achieve the sustained and controlled mild heating within the tumor tissues. First, the highest photothermal performance of AuNR@SiO
2
with 20-nm silica shell thickness than 5 or 40 nm was confirmed in vitro and in vivo. Then, the optimal conditions for precision immunotherapy were further investigated to produce mild temperature (44 to 45 °C) accurately in tumor tissues. The optimal conditions with AuNR@SiO
2
result in a distinct cell death with high early/late apoptosis and low necrosis, leading to very efficient ICD compared to lower or higher temperatures. In colon tumor-bearing mice, intratumorally injected AuNR@SiO
2
efficiently promotes a mild temperature within the tumor tissues by local irradiation of near-infrared (NIR) laser. This mild PTT substantially increases the population of mature dendritic cells (DCs) and cytotoxic T cells (CTLs) within tumor tissues, ultimately reversing the ITM into an immune-responsive milieu. Furthermore, we found that the combination mild PTT with AuNR@SiO
2
and anti-PD-L1 therapy could lead to the 100% complete regression of primary tumors and immunological memory to prevent tumor recurrence. Collectively, this study demonstrates that AuNR@SiO
2
with a robust methodology capable of continuously inducing mild temperature accurately within the ITM holds promise as an approach to achieve the precision photothermal immunotherapy.
Low sheet resistance buried metal bit line realized by high-temperature metal CVD process in vertical channel transistor array
Authors: Tian Chao , Sun Jiabao , Ping Yanlei , Wang Naizheng , Han Baodong , Liu Zhao , Li Yongjie , Meng Jingheng , Sun Hongbo , Wang Guilei , Chu Jian , Shao Guangsu , Shen Jie , Qiu Yunsong , Park Ted , Xiao Deyuan , Yoo Abraham , Zhao Chao 
Published: March 2024

Abstract
With the continuous evolution of dynamic random access memory (DRAM) devices, there is a growing demand for increased storage density per unit area. In this work, we aim to create a high-density array of vertical channel transistors using advanced DRAM process technology. A thickness of SiO2 (X+3 nm) was determined for the protective layer, which shows the best-protecting effect. We employed CVD to grow thin Ti films on the array’s bottom. To reduce the resistance of the buried bit line (BBL), we formed a high-quality metal silicide using a thermal annealing process combined with self-align technology. Nanoprobe measurement results show an average resistance of approximately 60 Ω of the bit line of each cell transistor, where the low series resistance can improve device performance. Our work involved optimizing the protective layers and achieving high-performance BBLs, paving the way for the development of high-density DRAMs.
Síndrome de Burnout en el personal de enfermería durante la pandemia, 2021.
Authors: Orellana Aguilar Maria Lorena , Choque Quispe Jhannet , Quispe Aliendre Evelin , Hualca Wayer Cecilia Alejandra 
Published: March 2024

Introducción: El síndrome de Burnout o fatiga laboral, es considerado un factor de riesgo, porque incide en la calidad de vida y la salud mental de los individuos y en especial del personal de salud y más aún durante una pandemia, si bien en la segunda ola la letalidad ha sido menor, las actividades del personal de enfermería no han cesado; en este sentido el propósito del trabajo de investigación fue establecer el grado de Burnout en función al inventario de Maslach en el personal de enfermería. Metodología: el trabajo fue desarrollado en el marco del enfoque cuantitativo, y los diseños de cohorte transversal, descriptivo y prospectivo; participaron 69 enfermeras de distintos servicios de salud que voluntariamente completaron el cuestionario. Resultados: El 65,2 % de la población participante corresponde al sexo femenino; el personal del municipio que participaron en mayor proporción es de Quillacollo y Vinto; el 43,3 % desempeña sus labores principalmente en el turno mañana; las horas trabajadas en un 43,5 % son en promedio 8 horas; el 71,4 % refieren conocer del síndrome de Burnout; dentro de una autoevaluación reportan que el 16,9 % está estresado crónicamente; en función a las sub escalas se muestran que principalmente es de referencia una escala media con una puntuación de 34 a 66 puntos. Discusión: Se ha encontrado datos similares en función a una evaluación global con otros estudios, sin embargo, también se encuentra discrepancias referentes a la realización personal.
Effect of Cooperative Script Metacognitive Learning Model (CSM) on Cognitive Ability of Class X Students at SMAN 1 Cibadak
Authors: Hasanah Iswati , Ramdhan Billyardi , Rustandi Eris 
Published: March 2024

Background: This research is entitled The Effect of Cooperative Script Metacognitive Learning Model (CSM) on Students' Cognitive Ability at SMAN 1 Cibadak. Thesis, Department of Biology Education, Faculty of Teacher Training and Education, University of Muhammadiyah Sukabumi, 2013. The purpose of this study was to determine the effect of the application of the CSM learning model on students' cognitive abilities. Methods: The method used in this research was quasi-experimental method with research design of Nonequivalent Control Group Design. This research was conducted at SMAN 1 Cibadak in the academic year 2012/2013. Sampling technique in this study used purposive sampling. Two classes from seven classes of X grade students. One class received learning with CSM model as the experimental group and one class received learning with Cooperative Learning model as the control class. The research subjects totaled 75 students, 38 students in the experimental class and 37 students in the control class. The instrument used to collect data in this study was a multiple choice test in accordance with the cognitive indicators of biology on environmental pollution material. Results: The results of this study indicate that the CSM learning model has an effect on students' cognitive abilities significantly, seen from hypothesis testing using t-test calculations, obtained tcount = 3.83 and ttable of 1.99 with a significance level (α) = 0.05 and degrees of freedom 73. Because tcount> ttable, so H0 is rejected and H1 is accepted. Conclusion: Based on the results of the analysis of the difference in the average score of student learning outcomes at each cognitive level, it shows that the application of the CSM learning model has a significant effect on students' cognitive abilities.
Keywords : Cooperative Script Metacognitive, cognitive ability
Wage Equality in the Perspective of Justice for Women Workers
Authors: Lusiana Adela Nugraheni  , Nur Sabela Rahmawati  
Published: March 2024

Labor problems in the world are not limited to problems between workers or workers and companies. Another issue that requires special attention is wages. The problem of wages is often the main discourse on the equality of wages for male and female workers. The type of research used in compiling this paper is juridical-normative. The normative juridical research method is a library law research conducted by examining secondary data including primary legal materials and secondary legal materials Labor law has not yet accommodated equal wages. The equality defined in this law is limited to work placements. Even though in Government Regulation Number 36 of 2021 equality of wages has been guaranteed, However, with no legal basis above it, this PP does not have a strong legal basis. Thus, it can be judged that the Manpower Law is actually not aligned with international legal instruments and the spirit of a decent living in the 1945 Constitution. In terms of the policy aspects carried out by the Ministry of Manpower in the national strategy, it is not yet aligned with the final objectives achieved and requires optimization in the future. Labor regulations in Indonesia have not accommodated equal pay. Equality that is accommodated is only equal in terms of opportunity and access to employment. policy, the Ministry of Manpower has not yet accommodated equal wages as a goal in the global instrument. This condition is possible because national legal instruments have not regulated gender equality.
Keywords: Wages Equality, Employment, Women Workers