bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can molecular hydrogen supplementation reduce exercise-induced oxidative stress in healthy adults? A systematic review and meta-analysis
Authors: Li Yiting , Bing Renjie , Liu Meng , Shang Zhangyuting , Huang Yan , Zhou Kaixiang , Bao Dapeng , Zhou Junhong 
Published: March 2024

ObjectiveExercise-induced oxidative stress affects multiple neurophysiological processes, diminishing the exercise performance. Hydrogen (H2) can selectively reduce excessive free radicals, but studies observed its “dual effects” on exercise-induced oxidative stress, that is, increasing or decreasing the oxidative stress. Therefore, we here conducted a systematic review and meta-analysis to quantitatively assess the influence of H2 on exercise-induced oxidative stress in healthy adults.MethodsWe conducted a systematic review of publications across five databases. The following keywords were used for search strategy: [“hydrogen”[Mesh] or “molecular hydrogen” or “hydrogen rich water” or “hydrogen-rich water” or “hydrogen rich saline”] and [“Oxidative Stress”[Mesh] or “Antioxidative Stress” or “Oxidative Damage” or “Oxidative Injury” or “Oxidative Cleavage”] and [“randomized controlled trial”[Mesh] or “randomized” or “RCT”]. We included trials reporting the effects of H2 on exercise-induced oxidative stress and potential antioxidant capacity post-exercise in healthy adults. Additionally, subgroup analyses were conducted to explore how various elements of the intervention design affected those outcomes.ResultsSix studies, encompassing seven experiments with a total of 76 participants, were included in our analysis. Among these studies, hydrogen-rich water, hydrogen bathing, and hydrogen-rich gas were three forms used in H2 administration. The H2 was applied in different timing, including before, during, or after exercise only, both before and after exercise, and repeatedly over days. Single-dose, multi-dose within 1 day and/or multiple-dose over days were implemented. It was observed that compared to placebo, the effects of H2 on oxidative stress (diacron-reactive oxygen metabolites, d-ROMs) was not significant (SMD = −0.01, 95%CI-0.42 to 0.39, p = 0.94). However, H2 induced greater improvement in antioxidant potential capacity (Biological Antioxidant Potential, BAP) (SMD = 0.29, 95% CI 0.04 to 0.54, p = 0.03) as compared to placebo. Subgroup analyses revealed that H2 supplementation showed greater improvement (SMD = 0.52, 95%CI 0.16 to 0.87, p = 0.02) in the antioxidant potential capacity of intermittent exercises than continuous exercise.ConclusionH2 supplementation can help enhance antioxidant potential capacity in healthy adults, especially in intermittent exercise, but not directly diminish the levels of exercise-induced oxidative stress. Future studies with more rigorous design are needed to examine and confirm these findings.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=364123, Identifier CRD42022364123.
Field testing of recombinant subunit vaccines against Teladorsagia circumcincta in lambing ewes demonstrates a lack of efficacy in the face of a multi-species parasite challenge
Authors: Nisbet Alasdair J. , McNeilly Tom N. , Price Daniel R. G. , Bartley Yvonne , Oliver Margaret , McBean Dave , Andrews Leigh , Mitchell Gillian , Duncan Rachael , Brocklehurst Sarah , Kenyon Fiona 
Published: March 2024

IntroductionWe previously demonstrated efficacy of an 8-antigen recombinant subunit vaccine against a single species homologous Teladorsagia circumcincta challenge in lambs and in lambing ewes in pen trials. We subsequently demonstrated efficacy of a simplified, 2-antigen, version of this vaccine in lambs in pen trials. Here, we test both vaccines in lambing ewes in a field setting.MethodsIn the work presented here, 12 adjacent plots were seeded with a mixed infection of several common species of parasitic nematodes of sheep in temperate regions, including T. circumcincta. Ewes (n = 144), in groups of 12, grazed for 2 years on these plots and, in the first year, six of these groups of ewes were vaccinated with a 2-antigen prototype vaccine against T. circumcincta prior to mating and then again prior to lambing. In the following year these ewes were immunised again, this time with the 8-antigen prototype vaccine against T. circumcincta prior to mating and then prior to lambing. Throughout both seasons antigen-specific serum antibody levels in ewes and faecal worm egg counts (FEC) in ewes and their lambs were monitored, along with nematode species diversity at lambing.ResultsImmunised ewes produced elevated serum antibody levels to each of the vaccine antigens following immunisation but their FEC levels were not statistically significantly impacted by vaccination with either vaccine. FEC levels were also not impacted in lambs co-grazing the pastures with these immunised ewes. Nematode species diversity was not significantly impacted by vaccination in either year.DiscussionThe immunosuppressive effects of co-infecting gastrointestinal nematodes, the absence of vaccine cross-protection against co-infecting species and the influence of the periparturient relaxation in immunity probably all contributed to the inability of either vaccine to protect against T. circumcincta infection in field trials in the work presented here.
Ocular pharmacological and biochemical profiles of 6-thioguanine: a drug repurposing study
Authors: Trotta Maria Consiglia , Gesualdo Carlo , Lepre Caterina Claudia , Russo Marina , Ferraraccio Franca , Panarese Iacopo , Marano Ernesto , Grieco Paolo , Petrillo Francesco , Hermenean Anca , Simonelli Francesca , D’Amico Michele , Bucolo Claudio , Lazzara Francesca , De Nigris Filomena , Rossi Settimio , Platania Chiara Bianca Maria 
Published: March 2024

Introduction:The purine analog 6-thioguanine (6TG), an old drug approved in the 60s to treat acute myeloid leukemia (AML), was tested in the diabetic retinopathy (DR) experimental in vivo setting along with a molecular modeling approach.Methods:A computational analysis was performed to investigate the interaction of 6TG with MC1R and MC5R. This was confirmed in human umbilical vein endothelial cells (HUVECs) exposed to high glucose (25 mM) for 24 h. Cell viability in HUVECs exposed to high glucose and treated with 6TG (0.05–0.5–5 µM) was performed. To assess tube formation, HUVECs were treated for 24 h with 6TG 5 µM and AGRP (0.5–1–5 µM) or PG20N (0.5–1–5–10 µM), which are MC1R and MC5R antagonists, respectively. For the in vivo DR setting, diabetes was induced in C57BL/6J mice through a single streptozotocin (STZ) injection. After 2, 6, and 10 weeks, diabetic and control mice received 6TG intravitreally (0.5–1–2.5 mg/kg) alone or in combination with AGRP or PG20N. Fluorescein angiography (FA) was performed after 4 and 14 weeks after the onset of diabetes. After 14 weeks, mice were euthanized, and immunohistochemical analysis was performed to assess retinal levels of CD34, a marker of endothelial progenitor cell formation during neo-angiogenesis.Results:The computational analysis evidenced a more stable binding of 6TG binding at MC5R than MC1R. This was confirmed by the tube formation assay in HUVECs exposed to high glucose. Indeed, the anti-angiogenic activity of 6TG was eradicated by a higher dose of the MC5R antagonist PG20N (10 µM) compared to the MC1R antagonist AGRP (5 µM). The retinal anti-angiogenic effect of 6TG was evident also in diabetic mice, showing a reduction in retinal vascular alterations by FA analysis. This effect was not observed in diabetic mice receiving 6TG in combination with AGRP or PG20N. Accordingly, retinal CD34 staining was reduced in diabetic mice treated with 6TG. Conversely, it was not decreased in diabetic mice receiving 6TG combined with AGRP or PG20N.Conclusion:6TG evidenced a marked anti-angiogenic activity in HUVECs exposed to high glucose and in mice with DR. This seems to be mediated by MC1R and MC5R retinal receptors.
The patterns of inbreeding depression in food-deceptive Dactylorhiza orchids
Authors: Wróblewska Ada , Ostrowiecka Beata , Brzosko Emilia , Jermakowicz Edyta , Tałałaj Izabela , Mirski Paweł 
Published: March 2024

IntroductionInbreeding depression (ID) in food-deceptive plants have been reported previously, however, it has not been often proven that selfed seeds germinate better than outbred ones or that selfing affects ID. To resolved these issues, food-deceptive related Dactylorhiza majalis, D. incarnata var. incarnata and D. fuchsii orchids were investigated.MethodsHand pollination treatments and control pollination were conducted. Fruit set, number of seeds per fruit, seed length, number of well-developed seeds per fruit, and proportion of in vitro asymbiotic germination seeds, were analyzed in relation to inflorescence levels and used as fitness indicators for these orchids. The ID and pollen limitation were measured.ResultsThe lowest ID (δ = −1.000) was in D. majalis, and present in combination with a high pollen limitation in its populations. D. fuchsii showed higher ID (δ = 0.366), and D. incarnata var. incarnata weak ID (δ = 0.065), although ID varied between its fitness components. The seed number per fruit differed significantly between the treatments and the inflorescence levels in treatments.DiscussionThis study emphasizes that the breeding system rather than the flower position on the inflorescence shaped the quality and quantity of reproductive output. The ID and its effect on germination of food-deceptive orchid seeds undoubtedly played an important role.
Shallow wet irrigation reduces nitrogen leaching loss rate in paddy fields by microbial regulation and lowers rate of downward migration of leaching water: a 15N-tracer study
Authors: Chen Tianyi , Yang Xiaoming , Zuo Zheng , Xu Huijuan , Yang Xingjian , Zheng Xiangjian , He Shuran , Wu Xin , Lin Xueming , Li Yongtao , Zhang Zhen 
Published: March 2024

China consumes 35% of the world’s fertilizer every year; however, most of the nitrogen fertilizers, which are essential for rice cultivation, are not used effectively. In this study, factors affecting the nitrogen leaching loss rate were studied in typical soil and rice varieties in South China. The effects of various irrigation measures on rice growth and nitrogen leaching loss were investigated by conducting experiments with eight groups. These groups included traditional irrigation (TI) and shallow wet irrigation (SWI). The TI is a common irrigation method for farmers in South China, maintaining a water layer of 5-8 cm depth. For SWI, after establishing a shallow water layer usually maintaining at 1-2 cm, paddy is irrigated when the field water level falls to a certain depth, then this process is then repeat as necessary. The nitrogen distribution characteristics were determined using 15N isotope tracing. In addition, the effects of nitrification, denitrification, and microbial composition on soil nitrogen transformation at different depths were studied by microbial functional gene quantification and high-throughput sequencing. The results revealed that in the SWI groups, the total nitrogen leaching loss rate reduced by 0.3-0.8% and the nitrogen use efficiency (NUE) increased by 2.18-4.43% compared with those in the TI groups. After the 15N-labeled nitrogen fertilizer was applied, the main pathways of nitrogen were found to be related to plant absorption and nitrogen residues. Furthermore, paddy soil ammonia-oxidizing archaea were more effective than ammonia-oxidizing bacteria for soil ammonia oxidation by SWI groups. The SWI measures increased the relative abundance of Firmicutes in paddy soil, enhancing the ability of rice to fix nitrogen to produce ammonium nitrogen, thus reducing the dependence of rice on chemical fertilizers. Moreover, SWI enhanced the relative abundance of nirS and nosZ genes within surface soil bacteria, thereby promoting denitrification in the surface soil of paddy fields. SWI also promoted ammonia oxidation and denitrification by increasing the abundance and activity of Proteobacteria, Nitrospirae, and Bacteroidetes. Collectively, SWI effectively reduced the nitrogen leaching loss rate and increase NUE.
Exploring the prevalence and predictors of low resilience and likely PTSD in residents of two provinces in Canada during the 2023 wildfires
Authors: Adu Medard K. , Shalaby Reham , Agyapong Belinda , Dias Raquel da Luz , Agyapong Vincent I. O. 
Published: March 2024

BackgroundThe recent wildfires in Canada serve as a stark example of the substantial and enduring harm they cause to the health of individuals and communities. Assessing the prevalence and correlates of Post-traumatic stress disorder (PTSD) and low resilience is valuable for policymakers in public health.ObjectivesThe study aimed to assess the prevalence and predictors of low resilience and likely PTSD among subscribers of Text4Hope, an e-mental health program that delivered daily supportive messages to residents of Nova Scotia (NS) and Alberta(AB) during the recent wildfires.MethodData collection was through a self-administered online survey completed by residents of the affected regions of NS and AB from May 14 to June 23, 2023. Data were analyzed using Statistical Package for the Social Sciences.ResultsOut of 298 respondents, the prevalence of low resilience and likely PTSD in our sample were 52.0 and 39.3%, respectively. Unemployed respondents were about 3 times more likely to experience both low resilience and PTSD symptoms compared to those employed. Respondents with a history of mental health diagnosis were about 4 times more likely to experience likely PTSD compared to those with no history of mental health diagnosis.ConclusionThis study established that unemployment and a history of mental health diagnosis predicted likely PTSD, while unemployment was associated with low resilience during the wildfire. These findings offer insights for clinical interventions and the creation of psychosocial support programs for vulnerable populations.
Elucidating the Response of Diverse Faba Bean Genotypes to Powdery Mildew Infection
Authors: Villegas-Fernández Ángel M. , García Lucía , Barilli Eleonora , Rispail Nicolas , Rubiales Diego 
Published: March 2024

Faba bean (Vicia faba) is a temperate grain legume of major importance for food and feed. Powdery mildews are an important group of diseases in many crops, although in faba bean, it is still considered to be of only minor and local relevance. Here, we report the occurrence of powdery mildew in southern Spain, which was identified through ITS sequencing as Erysiphe trifolii. Resistance screenings allowed the identification of a wide range of responses to the disease, with accessions BPL-710 and ILB-4708 outstanding due to their high levels of resistance. Histological studies showed that the mechanisms of resistance may involve the inhibition of germination and impairment of fungal development, as shown by a limited number of primary and secondary hyphae compared to those of the susceptible accessions. This work permitted a better understanding of the interaction of faba bean and powdery mildew, laying the ground for breeding programs for resistance if needed in the future.
Diversity of Freshwater Mollusks from Lake Pampulha, Municipality of Belo Horizonte, Minas Gerais, Brazil
Authors: Coelho Paulo Ricardo Silva , Thiengo Silvana Carvalho , de Mendonça Cristiane Lafetá Furtado , de Oliveira Nathália Moreira Teodoro , dos Santos Sonia Barbosa , Caldeira Roberta Lima , Geiger Stefan Michael 
Published: March 2024

The artificially created Lake Pampulha, within the metropolitan area of Belo Horizonte, Minas Gerais State, lacks detailed information about its freshwater mollusks, representing a challenge for the assessment and conservation of this ecosystem. In this study, conducted during June and August 2021, we collected specimens on four different occasions and at five sampling points around the lake, using different sampling equipment, such as a shovel and a scoop. During these collections, we identified, enumerated and measured 1538 species of mollusks and additionally examined the presence of trematode larvae. We identified twelve species of fresh water bivalves and gastropods: Biomphalaria straminea, Biomphalaria kuhniana, Biomphalaria occidentalis, Drepanotrema cimex, Pomacea maculata, Stenophysa marmorata, Physa acuta, Gundlachia ticaga, Melanoides tuberculata, Pseudosuccinea columella, Omalonyx matheroni and Corbicula largillierti. Echinostome and strigeocercaria types of larval trematodes were detected in B. straminea. Notably, some species of mollusks have not previously been recorded at Lake Pampulha. The analyses revealed differences in the composition and abundance of species, highlighting the higher number of mollusk species in areas more impacted by human actions. This study expands our understanding of mollusk diversity at Lake Pampulha, and provides valuable data for longitudinal comparisons of water quality and considerations of the conservation of native species. Furthermore, it highlights the importance of choosing appropriate sampling equipment, depending on the research objectives. The presence of invasive species of medical and veterinary relevance as intermediate hosts of parasites reinforces the need for efficient environmental protection strategies to preserve this artificial, aquatic environment widely used by the local population and by tourists.
Archives historiques et sédimentaires des paysages lacustres du piedmont des Pyrénées (lacs de Barbazan et de Loures-Barousse, Haute Garonne, France)
Authors: Arricau Victor , Chapron Emmanuel 
Published: March 2024

Centré sur les lacs de Barbazan et de Loures-Barousse en Haute-Garonne (nord des Pyrénées françaises), ce travail illustre
comment les archives sédimentaires (d’origine glaciaire, lacustre ou fluviatile) des lacs résiduels, des paléolacs et des «néolacs »
permettent de compléter des approches géohistoriques et paysagères basées sur des archives iconographiques pour reconstituer l’évolution des paysages lacustres. Le couplage de ces approches méthodologiques permet de préciser l’évolution des paléopaysages lacustres sur le temps long et de mieux comprendre l’exposition des territoires de piedmont aux risques de crue. Sur le plan méthodologique, cette étude démontre également la pertinence de la méthodologie limnogéologique (sondeur de sédiments et
carottage gravitaire) pour l’étude des «néo » lacs, comme le lac artificiel de Loures-Barousse.
Le tourisme lacustre comme stratégie de diversification d’une destination touristique estampillée « montagne » : entre discours et réalité
Authors: Duval-Massaloux Mélanie , Mathieu Julien , Nikolli Alice 
Published: March 2024

L’article porte sur la communication des destinations touristiques et interroge les relations entre, d’une part, les stratégies promotionnelles impulsées par des acteurs départementaux du tourisme et, d’autre part, la manière dont les acteurs locaux s’approprient ce type de démarche et s’en font les relais. Pour ce faire, l’article met en perspective la stratégie «quatre lacs » développée par Savoie Mont Blanc Tourisme (SMBT) et la place actuellement occupée par les lacs périalpins dans les discours et les images produites et véhiculées par les offices de tourisme (OT) des départements de la Savoie et de la Haute-Savoie. À un instant t qui est celui de l’année 2019, l’article analyse dès lors dans quelle mesure les OT de ces deux départements, acteurs dans la construction de la destination touristique et prescripteurs d’activités touristiques, considèrent les lacs comme des ressources touristiques à mettre en avant auprès de leurs visiteurs. En travaillant à partir d’OT situés en différents endroits des deux départements (environnement urbain, environnement lacustre, zones rurales, hautes vallées alpines, stations de ski), plus ou moins proches sur le plan kilométrique d’un des quatre lacs du territoire «Savoie Mont Blanc » , il s’agit de questionner les échelles auxquelles les lacs sont identifiés comme faisant ressource et d’interroger dans quelle mesure les dynamiques engagées par SMBT en matière de création d’une «destination lacs » sont partagées et relayées par l’échelon local, ici envisagé par le prisme des OT. Après avoir exposé la stratégie «quatre lacs » développée par SMBT et précisé l’organisation des OT en Savoie et en Haute-Savoie,
l’article présente la méthodologie développée ainsi que les principaux résultats obtenus, mettant en avant des décalages entre la
stratégie portée par SMBT et la manière dont les lacs sont perçus par les OT comme faisant (ou non) ressource.
Kidney Stones Account for Increased Imaging Studies in Autosomal Dominant Polycystic Kidney Disease
Authors: Simmons Kathryn E. , Ullman Lawrence S. , Dahl Neera K. 
Published: March 2024

Key Points

Kidney stones are common in patients with autosomal dominant polycystic kidney disease compared with matched controls with CKD.The increase in imaging seen in patients with autosomal dominant polycystic kidney disease correlates with the kidney stone burden in these patients.

Background
Patients with autosomal dominant polycystic kidney disease (ADPKD) may have more imaging studies than patients with other forms of CKD. We characterized the imaging burden of patients with ADPKD relative to a CKD population to determine which factors lead to increased imaging in patients with ADPKD.

Methods
We retrospectively reviewed patients seen at Yale Nephrology between January 2012 and January 2021. We collected demographic, clinical, and imaging data through automated query and manual chart review. Eight hundred seven patients with ADPKD were matched to 4035 CKD controls on the basis of criteria of sex, race, ethnicity, CKD stage, hypertension, and diabetes, but not age. The number of abdominal imaging studies were compared between ADPKD and CKD groups, and the effect of kidney stone diagnosis was further evaluated. Chi-square and t tests were used to evaluate demographic variables, and Kruskal Wallis and negative binomial regression models were used to evaluate differences between abdominal imaging studies.

Results
Patients with ADPKD had a greater number of total abdominal imaging studies (P < 0.0001), ultrasounds (P < 0.0001), and magnetic resonance imagings (P = 0.02) compared with controls. In patients with preserved renal function (eGFR >60 ml/min per m2), these differences persisted. Kidney stones were significantly more common among patients with ADPKD (P < 0.0001). In multivariable assessment of imaging study counts using a negative binomial model controlling for kidney stones, ADPKD was no longer a significant predictor. In patients with ADPKD, pyelonephritis; cyst complications; lower eGFR; diabetes; coronary artery disease; kidney stones; lower body mass index; and being male, Black, and younger increased the likelihood of having more imaging studies.

Conclusions
The higher prevalence of abdominal imaging studies in patients with ADPKD correlated with the increased incidence of kidney stones observed in this population.
KOMBINASI FERMENTASI TEPUNG PUTAK DAN TEPUNG DAUN LAMTORO DALAM PAKAN TERHADAP PERTUMBUHAN DAN KELANGSUNGAN HIDUP IKAN BANDENG (Chanos chanos)
Authors: TAEK ARNOLDIANUS , Tjendanawangi Agnette , Djonu Asriati 
Published: March 2024

Penelitian bertujuan mengkaji pertumbuhan dan kelulushidupan ikan bandeng perlakukan kombinasi tepung putak hasil fermentasi dan tepung daun lamtoro hasil difermentasi. Penelitian ini dilakukan menggunakan metode eksperimen dan perancangannya menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan dan tiga ulangan. Perlakuan yang dicobakan adalah A: fermentasi Tepung putak 25 % dan fermentasi tepung daun lamtoro 75 %; Perlakuan B: fermentasi tepung putak 50 % dan fermentasi tepung daun lamtoro 50 %; Perlakuan C: fermentasi tepung putak 75 % dan fermentasi tepung daun lamtoro 25 %; Perlakuan D: fermentasi tepung putak 100 %; perlakuan E: fermentasi tepung daun lamtoro 100 %. Perlakuan terbaik adalah perlakuan penggunaan kombinasi fermentasi tepung putak 75% dan fermentasi tepung daun lamtoro 25% dalam pakan yang memberikan pertambahan bobot mutlak terbesar yaitu 15,96 g, pertumbuhan spesifik harian 1,83 g%/hari dan kelangsungan hidup 100%.