bluuebunny/crossref_metadata_2025_split huggingface dataset:
New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences
Authors: Uribe Juan E. , Kelava Samuel , Nava Santiago , Cotes-Perdomo Andrea P. , Castro Lyda R. , Rivera-Paéz Fredy A. , Perea Silvia , Mans Ben J. , Gofton Alexander , Teo Ernest J. M. , Zardoya Rafael , Barker Stephen C. 
Published: March 2024

Abstract
Background
Amblyomma is the third most diversified genus of Ixodidae that is distributed across the Indomalayan, Afrotropical, Australasian (IAA), Nearctic and Neotropical biogeographic ecoregions, reaching in the Neotropic its highest diversity. There have been hints in previously published phylogenetic trees from mitochondrial genome, nuclear rRNA, from combinations of both and morphology that the Australasian Amblyomma or the Australasian Amblyomma plus the Amblyomma species from the southern cone of South America, might be sister-group to the Amblyomma of the rest of the world. However, a stable phylogenetic framework of Amblyomma for a better understanding of the biogeographic patterns underpinning its diversification is lacking.

Methods
We used genomic techniques to sequence complete and nearly complete mitochondrial genomes –ca. 15 kbp– as well as the nuclear ribosomal cluster –ca. 8 kbp– for 17 Amblyomma ticks in order to study the phylogeny and biogeographic pattern of the genus Amblyomma, with particular emphasis on the Neotropical region. The new genomic information generated here together with genomic information available on 43 ticks (22 other Amblyomma species and 21 other hard ticks–as outgroup–) were used to perform probabilistic methods of phylogenetic and biogeographic inferences and time-tree estimation using biogeographic dates.

Results
In the present paper, we present the strongest evidence yet that Australasian Amblyomma may indeed be the sister-group to the Amblyomma of the rest of the world (species that occur mainly in the Neotropical and Afrotropical zoogeographic regions). Our results showed that all Amblyomma subgenera (Cernyomma, Anastosiella, Xiphiastor, Adenopleura, Aponomma and Dermiomma) are not monophyletic, except for Walkeriana and Amblyomma. Likewise, our best biogeographic scenario supports the origin of Amblyomma and its posterior diversification in the southern hemisphere at 47.8 and 36.8 Mya, respectively. This diversification could be associated with the end of the connection of Australasia and Neotropical ecoregions by the Antarctic land bridge. Also, the biogeographic analyses let us see the colonization patterns of some neotropical Amblyomma species to the Nearctic.

Conclusions
We found strong evidence that the main theater of diversification of Amblyomma was the southern hemisphere, potentially driven by the Antarctic Bridge's intermittent connection in the late Eocene. In addition, the subgeneric classification of Amblyomma lacks evolutionary support. Future studies using denser taxonomic sampling may lead to new findings on the phylogenetic relationships and biogeographic history of Amblyomma genus.

Graphical Abstract
Accelerating targeted mosquito control efforts through mobile West Nile virus detection
Authors: Varga Zsaklin , Bueno-Marí Rubén , Risueño Iranzo José , Kurucz Kornélia , Tóth Gábor Endre , Zana Brigitta , Zeghbib Safia , Görföl Tamás , Jakab Ferenc , Kemenesi Gábor 
Published: March 2024

Abstract
Background
Different mosquito control strategies have been implemented to mitigate or prevent mosquito-related public health situations. Modern mosquito control largely relies on multiple approaches, including targeted, specific treatments. Given this, it is becoming increasingly important to supplement these activities with rapid and mobile diagnostic capacities for mosquito-borne diseases. We aimed to create and test the applicability of a rapid diagnostic system for West Nile virus that can be used under field conditions.

Methods
In this pilot study, various types of adult mosquito traps were applied within the regular mosquito monitoring activity framework for mosquito control. Then, the captured specimens were used for the detection of West Nile virus RNA under field conditions with a portable qRT-PCR approach within 3–4 h. Then, positive samples were subjected to confirmatory RT-PCR or NGS sequencing in the laboratory to obtain genome information of the virus. We implemented phylogenetic analysis to characterize circulating strains.

Results
A total of 356 mosquito individuals representing 7 species were processed in 54 pools, each containing up to 20 individuals. These pools were tested for the presence of West Nile virus, and two pools tested positive, containing specimens from the Culex pipiens and Anopheles atroparvus mosquito species. As a result of subsequent sequencing, we present the complete genome of West Nile virus and Bagaza virus.

Conclusions
The rapid identification of infected mosquitoes is the most important component of quick response adulticide or larvicide treatments to prevent human cases. The conceptual framework of real-time surveillance can be optimized for other pathogens and situations not only in relation to West Nile virus. We present an early warning system for mosquito-borne diseases and demonstrate its application to aid rapid-response mosquito control actions.

Graphical Abstract
Intact lung tissue and bronchoalveolar lavage fluid are both suitable for the evaluation of murine lung microbiome in acute lung injury
Authors: Zheng Lijun , Liu Chengjun , Wang Hongjing , Zhang Jun , Mao Lejiao , Dong Xiaomei , Hu Siyao , Li Na , Pi Dandan , Qiu Jingfu , Xu Feng , Chen Chengzhi , Zou Zhen 
Published: March 2024

Abstract
Background
Accumulating clinical evidence suggests that lung microbiome is closely linked to the progression of pulmonary diseases; however, it is still controversial which specimen type is preferred for the evaluation of lung microbiome.

Methods and results
To address this issue, we established a classical acute lung injury (ALI) mice model by intratracheal instillation of lipopolysaccharides (LPS). We found that the bacterial DNA obtained from the bronchoalveolar lavage fluid (BALF), intact lung tissue [Lung(i)], lung tissue after perfused [Lung(p)], and feces of one mouse were enough for 16S rRNA sequencing, except the BALF of mice treated with phosphate buffer saline (PBS), which might be due to the biomass of lung microbiome in the BALF were upregulated in the mice treated with LPS. Although the alpha diversity among the three specimens from lungs had minimal differences, Lung(p) had higher sample-to-sample variation compared with BALF and Lung(i). Consistently, PCoA analysis at phylum level indicated that BALF was similar to Lung(i), but not Lung(p), in the lungs of mice treated with LPS, suggesting that BALF and Lung(i) were suitable for the evaluation of lung microbiome in ALI. Importantly, Actinobacteria and Firmicutes were identified as the mostly changed phyla in the lungs and might be important factors involved in the gut-lung axis in ALI mice. Moreover, Actinobacteria and Proteobacteria might play indicative roles in the severity of lung injury.

Conclusion
This study shows both Lung(i) and BALF are suitable for the evaluation of murine lung microbiome in ALI, and several bacterial phyla, such as Actinobacteria, may serve as potential biomarkers for the severity of ALI.
Harmonizing culture and consumer psychology: optimizing color schemes for children’s product design inspired by traditional ornaments
Authors: Liang Ling Lin , Hangeldiyeva Nazik 
Published: March 2024

AbstractColor is one of the vital components of product design and the choice of color combination is a critical factor that affects consumer reaction and purchase decisions. However, the impact of traditional colors on color extraction and product design has seldom been studied. The purpose of this study is to discover suitable colors from traditional patterns to create color schemes that will be used in the design of children’s products. Colors were extracted from five major traditional ornaments obtained from Turkmenistan’s national carpets, each representing one of the main Turkmen tribes, using tools like Photoshop, Adobe Color, and EasyRGB, with the objective of investigating psychological-emotional attachment, reaction, and attitudes towards the colors obtained. This initial extraction provided 124 color data points. Initially, extracted colors were refined and used to develop novel color schemes by two independent survey studies. The first survey, based on a sample of 104 parents of preschool children, identified colors that have a positive emotional effect on consumer preferences and provided the basis to develop color schemes. As a result, 25 colors were identified for use in developing 14 color schemes. The second survey, based on a sample of 48 parents of preschool children, identified which color schemes have an attraction for consumers. The survey indicated that 11 out of these 14 schemes were highly favored by the respondents. Based on these results, this study proposed new color schemes for children’s products, extracted from traditional patterns of Turkmenistan and aligned with the psychological perception of the consumers.
CNS-associated macrophages contribute to intracerebral aneurysm pathophysiology
Authors: Glavan Martina , Jelic Ana , Levard Damien , Frösen Juhana , Keränen Sara , Franx Bart A. A. , Bras Ana-Rita , Louet Estelle R. , Dénes Ádám , Merlini Mario , Vivien Denis , Rubio Marina 
Published: March 2024

AbstractIntracerebral aneurysms (IAs) are pathological dilatations of cerebral arteries whose rupture leads to subarachnoid hemorrhage, a significant cause of disability and death. Inflammation is recognized as a critical contributor to the formation, growth, and rupture of IAs; however, its precise actors have not yet been fully elucidated. Here, we report CNS-associated macrophages (CAMs), also known as border-associated macrophages, as one of the key players in IA pathogenesis, acting as critical mediators of inflammatory processes related to IA ruptures. Using a new mouse model of middle cerebral artery (MCA) aneurysms we show that CAMs accumulate in the IA walls. This finding was confirmed in a human MCA aneurysm obtained after surgical clipping, together with other pathological characteristics found in the experimental model including morphological changes and inflammatory cell infiltration. In addition, in vivo longitudinal molecular MRI studies revealed vascular inflammation strongly associated with the aneurysm area, i.e., high expression of VCAM-1 and P-selectin adhesion molecules, which precedes and predicts the bleeding extent in the case of IA rupture. Specific CAM depletion by intracerebroventricular injection of clodronate liposomes prior to IA induction reduced IA formation and rupture rate. Moreover, the absence of CAMs ameliorated the outcome severity of IA ruptures resulting in smaller hemorrhages, accompanied by reduced neutrophil infiltration. Our data shed light on the unexplored role of CAMs as main actors orchestrating the progression of IAs towards a rupture-prone state.
Graphical abstract
Comparison of 3D-printed and laboratory-fabricated Hyrax on stress distribution and displacement of the maxillary complex: a 3D finite element study
Authors: Bocklet Michael , Ahmadi Farhad , Tremont Timothy , Ross Loring , Yao Hai , Andrade Ildeu 
Published: March 2024

Abstract
Objective
To analyze and compare the effects of a traditional laboratory-fabricated Hyrax expander (T-Hyrax) and two different 3D-printed Hyrax expander models relative to tension points, force distribution, and areas of concentration in the craniofacial complex during maxillary expansion using finite element analysis.

Materials and methods
Three maxillary expanders with similar designs, but various alloys were modeled: a T-Hyrax, a fully printed Hyrax (F-Hyrax), and a hybrid printed Hyrax (H-Hyrax). The stress distributions and magnitude of displacements were assessed with a 5 mm expansion in a symmetrical finite element model. The areas of interest included the teeth, alveolar processes, midpalatal suture, nasal complex, circummaxillary sutures (CS), and the expanders themselves.

Results
The highest stress value (29.2 MPa) was found at the midpalatal suture of the F-Hyrax, while the lowest stress (0.90 MPa) was found at the temporozygomatic suture in the T-Hyrax. On average, the F-Hyrax increased stress at the CS by 24.76% compared with the T-Hyrax and H-Hyrax. The largest displacements were found at the upper incisor (U1) and anterior nasal spine (ANS). The findings indicated an average increase of 12.80% displacement at the CS using the F-Hyrax compared to the T-Hyrax.

Conclusion
The F-Hyrax exerts more stress and displacement on the maxilla than both the T-Hyrax and H-Hyrax, where the weak link appears to be the solder joint.
An elementary review on basic principles and developments of qubits for quantum computing
Authors: Chae Eunmi , Choi Joonhee , Kim Junki 
Published: March 2024

AbstractAn elementary review on principles of qubits and their prospects for quantum computing is provided. Due to its rapid development, quantum computing has attracted considerable attention as a core technology for the next generation and has demonstrated its potential in simulations of exotic materials, molecular structures, and theoretical computer science. To achieve fully error-corrected quantum computers, building a logical qubit from multiple physical qubits is crucial. The number of physical qubits needed depends on their error rates, making error reduction in physical qubits vital. Numerous efforts to reduce errors are ongoing in both existing and emerging quantum systems. Here, the principle and development of qubits, as well as the current status of the field, are reviewed to provide information to researchers from various fields and give insights into this promising technology.
Slip distribution of the 2024 Noto Peninsula earthquake (MJMA 7.6) estimated from tsunami waveforms and GNSS data
Authors: Fujii Yushiro , Satake Kenji 
Published: March 2024

AbstractThe 1 January 2024 Noto-Hanto (Noto Peninsula) earthquake (MJMA 7.6) generated strong ground motion, large crustal deformation and tsunamis that caused significant damage in the region. Around Noto Peninsula, both offshore submarine and partially inland active faults have been identified by previous projects: Ministry of Land, Infrastructure, Transport and Tourism (MLIT) and Japan Sea Earthquake and Tsunami Research Project (JSPJ). We inverted the tsunami waveforms recorded on 6 wave gauges and 12 tide gauges around Sea of Japan and the GNSS data recorded at 53 stations in Noto Peninsula to estimate the slip amount and seismic moment on each of active faults. The results show that the 2024 coseismic slips were 3.5 m, 3.2 m, and 3.2 m on subfaults NT4, NT5 and NT6 of the JSPJ model, located on the northern coast of Noto Peninsula and dipping toward southeast. A smaller slip, 1.0 m, estimated on NT8 on the southwestern end of the 2024 rupture, may be attributed to its previous rupture during the 2007 Noto earthquake. The total length of these four faults is ~ 100 km, and the seismic moment is 1.90 × 1020 Nm (Mw = 7.5). Almost no slip was estimated on the northeastern subfaults NT2 and NT3, which dip northwestward, opposite to NT4–NT5–NT6, and western subfault NT8. Aftershocks including the MJMA 6.1 event occurred in the NT2–NT3 region, but they are smaller than the potential magnitude (Mw 7.1) those faults can release in a tsunamigenic earthquake. Similar features are also found for the MLIT model; the 2024 slip was only on F43 along the northern coast of Noto Peninsula, and northeastern F42 did not rupture, leaving potential for future event.
Graphical Abstract
Extended radical resection and chest wall reconstruction for a pulmonary sarcomatoid carcinoma: a case report
Authors: Zhao Yingzhi , Xie Shaohua , Zheng Haoqian , Zhang Kaixin , Gao Xin , Liu Wenwu , Dai Wei , Yu Hongfan , Shi Qiuling , Hu Bin , Li Qiang , Xie Tianpeng , Wei Xing 
Published: March 2024

Abstract
Background
Pulmonary sarcomatoid carcinoma (PSC) is a rare and highly malignant type of non-small cell lung cancer (NSCLC), for which the treatment of choice is surgery. For peripheral PSC growing outward and invading the chest wall, a complete resection of the affected lung lobes and the invaded chest wall can improve long-term prognosis. However, when the extent of the resected chest wall is large, reconstruction is often required to reduce the risk of postoperative complications. Here, we present a case of PSC invading the chest wall treated with successful extended radical resection for lung cancer and chest wall reconstruction.

Case presentation
A 58-year-old male patient with a nodule in the right upper lobe that had been identified on physical examination 2 years before presentation presented to our hospital with a recent cough, expectoration, and chest pain. Imaging revealed a mass in the right upper lobe that had invaded the chest wall. Preoperative puncture pathology revealed poorly differentiated NSCLC. We performed extended radical resection for lung cancer under open surgery and reconstructed the chest wall using stainless steel wire and polypropylene meshes. The procedure was uneventful, and the patient was discharged 7 days postoperatively. Furthermore, the final pathology revealed PSC.

Conclusions
This case underscores the feasibility of surgical R0 resection in patients with PSC with chest wall invasion and no lymph node metastasis, potentially enhancing long-term outcomes. The novel aspect of this case lies in the individualized chest wall reconstruction for a large defect, using cost-effective materials that offered satisfactory structural support and postoperative recovery, thereby providing a valuable reference for similar future surgical interventions.
Measurement properties of the falls efficacy scale in patients on hemodialysis
Authors: de Jesus Luciana Angélica da Silva , Pinheiro Bruno Valle , Almeida Ana Beatriz Laguardia , da Cunha Lara Fagundes Fonseca , Resende Gabriela Moreira , Moreira-Júnior Marco Aurelio , Campos-Junior Mauricio Becho , Koufaki Pelagia , Lucinda Leda Marília Fonseca , Reboredo Maycon Moura 
Published: March 2024

Abstract
Background
The measurement properties of the falls efficacy scale have not been evaluated in patients on hemodialysis. This study determined the inter- and intrarater reliability, standard error of measurement, minimal detectable change, and limits of agreements of the falls efficacy scale in patients on hemodialysis.

Methods
A cross-sectional study was conducted with 65 patients (57.5 ± 13.9 years, 63.1% male) on hemodialysis. The fear of falling was assessed by two previously trained raters using the falls efficacy scale. The intraclass correlation coefficient, standard error of measurement, minimal detectable change, and Bland–Altman plot were calculated to assess the inter- and intrarater reliability of the falls efficacy scale.

Results
The interrater intraclass correlation coefficient was 0.91, and the intrarater intraclass correlation coefficient was 0.78, representing excellent interrater and good intrarater reliability. The standard error of measurement for inter- and intrarater assessments were 2.99 and 4.46, and the minimal detectable change for inter- and intrarater assessments were 9.26 and 12.33, respectively. The interrater mean difference score was 0.26 (95% limits of agreement: − 8.01 to 8.53), and the intrarater mean difference score was − 1.06 (95% limits of agreement: − 13.39 to 11.27).

Conclusion
In patients on hemodialysis, the falls efficacy scale showed excellent and good inter- and intrarater reliability, respectively. Additionally, standard error of measurement, minimal detectable change, and limits of agreements of the falls efficacy scale score were satisfactory.
Individual differences in emerging adults’ spatial abilities: What role do affective factors play?
Authors: Desme Carlos J. , Dick Anthony S. , Hayes Timothy B. , Pruden Shannon M. 
Published: March 2024

AbstractSpatial ability is defined as a cognitive or intellectual skill used to represent, transform, generate, and recall information of an object or the environment. Individual differences across spatial tasks have been strongly linked to science, technology, engineering, and mathematics (STEM) interest and success. Several variables have been proposed to explain individual differences in spatial ability, including affective factors such as one’s confidence and anxiety. However, research is lacking on whether affective variables such as confidence and anxiety relate to individual differences in both a mental rotation task (MRT) and a perspective-taking and spatial orientation task (PTSOT). Using a sample of 100 college students completing introductory STEM courses, the present study investigated the effects of self-reported spatial confidence, spatial anxiety, and general anxiety on MRT and PTSOT. Spatial confidence, after controlling for effects of general anxiety and biological sex, was significantly related to performance on both the MRT and PTSOT. Spatial anxiety, after controlling for effects of general anxiety and biological sex, was not related to either PTSOT or MRT scores. Together these findings suggest some affective factors, but not others, contribute to spatial ability performance to a degree that merits advanced investigation in future studies.
First-year students AI-competence as a predictor for intended and de facto use of AI-tools for supporting learning processes in higher education
Authors: Delcker Jan , Heil Joana , Ifenthaler Dirk , Seufert Sabine , Spirgi Lukas 
Published: March 2024

AbstractThe influence of Artificial Intelligence on higher education is increasing. As important drivers for student retention and learning success, generative AI-tools like translators, paraphrasers and most lately chatbots can support students in their learning processes. The perceptions and expectations of first-years students related to AI-tools have not yet been researched in-depth. The same can be stated about necessary requirements and skills for the purposeful use of AI-tools. The research work examines the relationship between first-year students’ knowledge, skills and attitudes and their use of AI-tools for their learning processes. Analysing the data of 634 first-year students revealed that attitudes towards AI significantly explains the intended use of AI tools. Additionally, the perceived benefits of AI-technology are predictors for students’ perception of AI-robots as cooperation partners for humans. Educators in higher education must facilitate students’ AI competencies and integrate AI-tools into instructional designs. As a result, students learning processes will be improved.