bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perfluoroalkyl compounds in relation to life-history and reproductive parameters in bottlenose dolphins (Tursiops truncatus) from Sarasota Bay, Florida, USA
Authors: Houde Magali , Balmer Brian C. , Brandsma Sicco , Wells Randall S. , Rowles Teri K. , Solomon Keith R. , Muir Derek C. G. 
Published: August 2006

Abstract
Perfluoroalkyl compounds (PFCs) were determined in plasma, milk, and urine of free-ranging bottlenose dolphins (Tursiops truncatus) from Sarasota Bay (FL, USA) during three winter and two summer capture-and-release programs (2002-2005). Plasma and urine samples were extracted using an ion-pairing method. Perfluoroalkyl compounds were extracted from milk samples using acetonitrile, and extracts were cleaned with graphitized nonporous carbon. All extracts were analyzed by high-performance liquid chromatography–tandem mass spectrometry. Mean seasonal sum of PFCs (ΣPFCs) detected in dolphin plasma ranged from 530 to 927 ng/g wet weight. No significant differences (p &gt; 0.05) were found in concentrations between seasons, suggesting a constant exposure to PFCs. Overall, blubber thickness of dolphins did not correlate with PFC concentrations in plasma, suggesting an absence of PFC sequestration in blubber. Sexually immature calves (age, &lt;10 years; mean ΣPFCs, 1,410 ± 780 ng/g wet wt) were significantly more contaminated (p &lt; 0.001) than their mothers (mean ΣPFCs, 366 ± 351 ng/g wet wt). The reproductive history of females had a significant role in the burden of PFC contamination; PFC concentrations in nulliparous females (females that have not been observed with calves) were significantly greater than those detected in uniparous females (females that have been observed with one calf), suggesting an off-loading of PFCs during or after parturition. To investigate this hypothesis, PFCs were analyzed in milk samples (n = 10; mean ΣPFCs, 134 ± 76.1 ng/g wet wt), confirming a maternal transfer of PFCs through lactation in dolphins. Results from the present study showed that young and developing bottlenose dolphins are highly exposed to PFCs. These chemicals also were detected in urine (mean ΣPFCs, 26.6 ± 79 ng/g wet wt), indicating that the urinary system is an important pathway of PFC depuration in dolphins.
Chlorine toxicity to early life stages of freshwater mussels (Bivalvia: Unionidae)
Authors: Valenti Theodore W. , Cherry Donald S. , Currie Rebecca J. , Neves Richard J. , Jones Jess W. , Mair Rachel , Kane Cynthia M. 
Published: August 2006

Abstract
Chlorine (Cl) is a highly toxic, widely used halogen disinfectant that is present in point-source pollution discharges from wastewater treatment plants and industrial facilities. The U.S. Environmental Protection Agency freshwater criteria for Cl are 19 μg total residual Cl (TRC)/L as a maximum 1-h average concentration and 11 μg TRC/L as a maximum 4-d average; however, toxicological data for unionids were not used in these calculations. To address this void in the data, we conducted acute tests with glochidia from several species and 21-d bioassays with three-month-old Epioblasma capsaeformis and three-, six-, and 12-month-old Villosa iris juveniles. The 24-h lethal concentration 50 values for glochidia were between 70 and 220 μg TRC/L, which are 2.5 to 37 times higher than those reported in other studies for cladocerans. Significant declines in growth and survivorship were observed in the 21-d test with E. capsaeformis at 20 μg TRC/L. Lowest-observed-adverse-effects concentrations in bioassays with juvenile V. iris were higher (30–60 μg TRC/L) but showed a significant trend of declining toxicity with increased age. Although endpoints were above water quality criteria, the long life spans of unionids and potential implications of chronic exposure to endangered juvenile mussels still warrant concern.
Leaching of copper, chromium, and boron from treated timber during aboveground exposure
Authors: García-Valcárcel Ana I. , Tadeo José L. 
Published: August 2006

Abstract
Field studies were conducted to evaluate leaching of Cu, Cr, and B from timber treated with a Cr, Cu, and B wood preservative and exposed aboveground in Spanish weathering conditions during one year with a total rainfall of approximately 500 mm. The effect of timber orientation (horizontal for decks and vertical for fences) on metal leaching was assessed. Leaching of metals after one year was higher for decks than for fences, with total amounts of metals leached being 226.6 and 87.8 mg/m2 for Cu, 199.5 and 42.4 mg/m2 for Cr, and 110.1 and 32.6 mg/m2 for B from decks and fences, respectively. Leaching rates did not drop constantly with time, because an increase in metal leaching, except for B, was observed at the end of the year after a long dry period. The order of metal emission varied with cumulative rainfall: Leaching of B was higher than Cr initially, but the opposite occurred at the end of the assay. Total emissions of Cu and Cr obtained in a laboratory assay were lower than those for decks and fences under field conditions when expressed per unit surface area (mg/m2), but they were similar to the values obtained for fences when expressed as a percentage of the initial amount. However, the percentage of B leached from wood was higher in laboratory than in field assays for both orientations, with the amount of B leached per unit surface area under laboratory conditions being higher than that leached from fences but lower than that leached from decks. Emission rates extrapolated to one year from laboratory data underestimated metal leaching from decks and overestimated emissions from fences.
Geckos as indicators of mining pollution
Authors: Fletcher Dean E. , Hopkins William A. , Saldaña Teresa , Baionno Jennifer A. , Arribas Carmen , Standora Michelle M. , Fernández-Delgado Carlos 
Published: August 2006

Abstract
Catastrophic collapse of a mine tailings dam released several million cubic meters of toxic mud and acidic water into the Guadiamar River valley, southern Spain, in 1998. Remediation efforts removed most of the sludge from the floodplain, but contamination persists. Clean-up activities also produced clouds of aerosolized materials that further contaminated the surrounding landscape. Whole-body concentrations of 21 elements in the Moorish wall gecko, Tarentola mauritanica, a common inhabitant of both rural and urban areas, were compared among seven locations. Locations spanned an expected contamination gradient and included a rural and an urban non–mine-affected location, two mine-affected towns, and three locations on the contaminated floodplain. Multivariate analyses of whole-body concentrations identified pollutants that increased across the expected contamination gradient, a trend particularly evident for As, Pb, and Cd. Additionally, higher contaminant concentrations occurred in prey items eaten by geckos from mine-affected areas. Comparison of element concentrations in tails and whole bodies suggests that tail clips are a viable nondestructive index of contaminant accumulation. Our results indicate that areas polluted by the mine continue to experience contamination of the terrestrial food chain. Where abundant, geckos represent useful taxa to study the bioavailability of some hazardous pollutants.
Influence of water quality and associated contaminants on survival and growth of the endangered Cape Fear shiner (Notropis mekistocholas)
Authors: Hewitt Amanda H. , Cope W. Gregory , Kwak Thomas J. , Augspurger Tom , Lazaro Peter R. , Shea Damian 
Published: August 2006

AbstractThe Cape Fear shiner (Notropis mekistocholas) is a recently described cyprinid species endemic to the Cape Fear River Basin of North Carolina, USA. Only five populations of the fish remain; thus, it is listed as endangered by the U.S. Government. Determining habitat requirements of the Cape Fear shiner, including water quality and physical habitat, is critical to the survival and future restoration of the species. To assess water quality in the best remaining and in the historical habitats, we conducted a 28-d in situ bioassay with captively propagated Cape Fear shiners. Fish were deployed at 10 sites in three rivers, with three cages per site and 20 fish per cage. Water and sediment samples were collected and analyzed for selected metals and organic contaminants. Passive sampling devices also were deployed at each site and analyzed for organic contaminants at test termination. Fish survival, growth (as measured by an increase in total length), and contaminant accumulation were measured on completion of the bioassay. Survival of caged fish averaged 76% (range, 53–100%) and varied significantly among sites and rivers. Caged fish accumulated quantities of cadmium, mercury, polychlorinated biphenyls, and other persistent contaminants over the test duration and grew significantly at only four sites. No apparent relations were observed between exposure to or accumulation of a specific contaminant and reduced growth or survival of fish among all the sites. However, a generalized hazard assessment showed that certain sites exhibited trends in cumulative contaminant presence with reduced fish survival and growth, thereby enabling the identification of the existing riverine habitat most suitable for reintroduction or population augmentation of this endangered fish.
Seasonality effects on pharmaceuticals and s-triazine herbicides in wastewater effluent and surface water from the Canadian side of the upper Detroit River
Authors: Hua Wen Yi , Bennett Erin R. , Maio Xui-Sheng , Metcalfe Chris D. , Letcher Robert J. 
Published: August 2006

Abstract
The influence of seasonal changes in water conditions and parameters on several major pharmacologically active compounds (PhACs) and s-triazine herbicides was assessed in the wastewater and sewage treatment plant (WSTP) effluent as well as the downstream surface water from sites on the Canadian side of the upper Detroit River, between the Little River WSTP and near the water intake of a major drinking water treatment facility for the City of Windsor (ON, Canada). The assessed PhACs were of neutral (carbamazepine, cotinine, caffeine, cyclophosphamide, fluoxetine, norfluoxetine, pentoxifylline, and trimethoprim) and acidic (ibuprofen, bezafibrate, clofibric acid, diclofenac, fenoprofen, gemfibrozil, indomethacin, naproxen, and ketoprofen) varieties. The major assessed s-triazine herbicides were atrazine, simazine, propazine, prometon, ametryn, prometryn, and terbutryn. At sampling times from September 2002 to June 2003, 15 PhACs were detected in the WSTP effluent at concentrations ranging from 1.7 to 1,244 ng/L. The PhAC concentrations decreased by as much 92 to 100% at the Little River/Detroit River confluence because of the river dilution effect, with further continual decreases at sites downstream from the WSTP. The only quantifiable s-triazine in WSTP effluent, atrazine, ranged from 6.7 to 200 ng/L and was higher in Detroit River surface waters than in WSTP effluent. Only carbamazepine, cotinine, and atrazine were detectable at the low-nanogram and subnanogram levels in surface waters near a drinking water intake site. Unlike the PhACs, atrazine in the Detroit River is not attributable to point sources, and it is heavily influenced by seasonal agricultural usage and runoff. Detroit River surface water concentrations of carbamazepine, cotinine, and atrazine may present a health concern to aquatic wildlife and to humans via the consumption of drinking water.
Biomagnification of polybrominated diphenyl ether and hexabromocyclododecane flame retardants in the polar bear food chain in Svalbard, Norway
Authors: Sørmo Eugen Gravningen , Salmer Maria Pettersvik , Jenssen Bjørn Munro , Hop Haakon , Bæk Kine , Kovacs Kit Maureen , Lydersen Christian , Falk-Petersen Stig , Gabrielsen Geir Wing , Lie Elisabeth , Skaare Janneche Utne 
Published: August 2006

Abstract
Concentrations of brominated flame retardants (BFRs), including polybrominated diphenylethers (PBDEs) and hexabromocyclododecane (HBCD), were investigated in an arctic marine food chain consisting of four invertebrate species: polar cod (Boreogadus saida), ringed seals (Pusa hispida), and polar bears (Ursus maritimus). The most abundant BFR, brominated diphenyl ether (BDE)-47, was found in detectable concentrations even in zooplankton, the lowest trophic level examined in this study. Most of the investigated BFRs biomagnified as function of tropic level in the food chain. A noticeable exception occurred at the highest trophic level, the polar bear, in which only BDE-153 was found to increase from its main prey, the ringed seal, indicating that polar bears appear to be able to metabolize and biodegrade most BFRs. In contrast, lower-brominated PBDEs, particularly BDE-47, showed clear signs of bioaccumulation in zooplankton, polar cod, and ringed seals. We suggest that this discrepancy in the fate of BFRs among the different species may be related to greater induction of oxidative detoxification activities in the polar bear. Absorption and debromination rates may be more important for bioaccumulation rates of BFRs in zooplankton, polar cod, and ringed seals. Lipid weight–based concentrations (LWCs) and whole body–based concentrations (WBCs) of BFRs were used to assess biomagnification factors (BMFs). Whole-body concentrations gave the most realistic BMFs, as BMFs derived from LWCs seem to be confounded by the large variability in lipid content of tissues from the investigated species. This study demonstrates that PBDEs and HBCD have reached measurable concentrations even in the lower trophic levels (invertebrates and fish) in the Arctic and biomagnifies in the polar bear food chain.
Effects of cyanobacterial toxins and cyanobacterial cell-free crude extract on germination of alfalfa (Medicago sativa) and induction of oxidative stress
Authors: Pflugmacher Stephan , Jung Katharina , Lundvall Linn , Neumann Stefanie , Peuthert Anja 
Published: August 2006

Abstract
Cyanobacterial toxins have adverse effects on both terrestrial and aquatic plants. Microcystins are cyclic heptapeptides and an important group of cyanotoxins. When lake water contaminated with cyanobacterial blooms is used for spray irrigation, these toxins can come in contact with agricultural plants. During the exposure to these toxins, reactive oxygen species can form. These reactive oxygen species have a strong reactivity and are able to interact with other cellular compounds (lipids, protein, and DNA). Plants have antioxidative systems that will limit the negative effects caused by reactive oxygen species. These systems consist of enzymes, such as superoxide dismutase, catalase, and ascorbate peroxidase, and nonenzymatic substances, such as reduced glutathione or vitamins. The aim of the present study was to investigate the effects of cyanobacterial toxins (microcystins and anatoxin-a) and cyanobacterial cell-free crude extract on alfalfa (Medicago sativa) seedlings. Inhibition of germination and root growth was observed with toxin concentrations of 5.0 μg/L. Also, oxidative damage, such as lipid peroxidation, was detected after the exposure of alfalfa seedlings to the toxin. Reactive oxygen detoxifying enzymes were elevated, showing a marked response in alfalfa to oxidative stress caused by the exposure to cyanobacterial metabolites that might influence the growth and development of these plants negatively.
Perfluorinated compounds in streams of the Shihwa industrial zone and Lake Shihwa, South Korea
Authors: Rostkowski Pawel , Yamashita Nobuyoshi , So Iris Man Ka , Taniyasu Sachi , Lam Paul Kwan Sing , Falandysz Jerzy , Lee Kyu Tae , Kim Seung Kyu , Khim Jong Seong , Im Sook Hyeon , Newsted John L. , Jones Paul D. , Kannan Kurunthachalam , Giesy John P. 
Published: August 2006

Abstract
Concentrations of perfluorinated alkyl compounds (PFAs), including perfluorooctane sulfonate (PFOS), perfluorohexanesulfonate, perfluorobutanesulfonate, perfluorooctanesulfonamide, perfluorodecanoate, perfluorononanoic acid, perfluorooctanoate (PFOA), perfluoroheptanoate, and perfluorohexanoate, were measured in the streams of the Shihwa and Banweol industrial areas on the west coast of South Korea as well as the adjacent Lake Shihwa (an artificial lake) and Gyeonggi Bay. Perfluorinated alkyl compounds were concentrated from water using solid-phase extraction and were identified and quantified by liquid chromatography/triple-quadrapole tandem mass spectrometry. Of the PFAs measured, PFOS and PFOA occurred at the greatest concentrations. Concentrations of PFOS ranged from 2.24 to 651 ng/L, and concentrations of PFOA ranged from 0.9 to 62 ng/L. The concentrations of PFOS observed in Lake Shihwa were among the greatest ever measured in the environment. These results suggest local industrial sources of PFOS and PFOA as well as other PFAs. Because of dilution, the greatest concentrations occur in a rather restricted area, near the points of discharge of the streams that empty into the lake. The greatest measured concentration of PFOS exceeded the threshold for effects predicted for predatory birds consuming aquatic organisms continuously exposed to this level.
Toxicity models of pulsed copper exposure toPimephales promelasandDaphnia magna
Authors: Butcher Jonathan , Diamond Jerry , Bearr Jonathan , Latimer Henry , Klaine Stephen J. , Hoang Tham , Bowersox Marcus 
Published: August 2006

AbstractSemiempirical models are useful for interpreting the response of aquatic organisms to toxicants as a function of exposure concentration and duration. Most applications predict cumulative mortality at the end of the test for constant exposure concentrations. Summary measures, such as the median lethal concentration, are then estimated as a function of concentration. Real-world exposures are not constant. Effects may depend on pulse timing, and cumulative analysis based only on integrated exposure concentration is not sufficient to interpret results. We undertook a series of pulsed-exposure experiments using standard toxicological protocols and interpreted the results (mortality, biomass, and reproduction) using a dynamic generalization of a Mancini/Breck-type model that includes two compartments, one for internal concentration as a function of exposure and one for site-of-action concentration or accumulated damage as a function of the internal dose. At exposure concentrations near the effects level, the model explained approximately 50% of the variability in the observed time history of survival, 43% of the change in biomass, and 83% of the variability in net reproduction. Unexplained variability may result from differences in organism susceptibility, amplified by the effects of small sample sizes in standard tests. The results suggest that response is sensitive to prior conditions and that constant-exposure experiments can underestimate the risk from intermittent exposures to the same concentration. For pulsed exposures, neither the average nor the maximum concentration alone is an adequate index of risk, which depends on both the magnitude, duration, and timing of exposure pulses. Better understanding about the impacts of pulsed exposures will require use of experimental protocols with significantly greater numbers of replicates.
Characterization of Pb, Cu, and Cd adsorption on particulate organic matter in soil
Authors: Guo Xueyan , Zhang Shuzhen , Shan Xiao-quan , Luo Lei , Pei Zhiguo , Zhu Yong-Guan , Liu Tao , Xie Ya-ning , Gault Andrew 
Published: August 2006

Abstract
Evidence exists for significant metal enrichment in particulate organic matter (POM) compared to other soil constituents, but the relevant mechanisms are poorly understood. In the present study, adsorption of the heavy metals Pb, Cu, and Cd on a loamy soil and on POM separated from this soil was investigated. The adsorption kinetic data can be well described with a pseudo–second order model, whereas the equilibrium data are well fitted by a Langmuir model. Adsorption isotherms and kinetics data, in addition to the influence of pH on metal adsorption, showed that POM had a much higher adsorption capacity for Pb, Cu, and Cd compared to the whole soil. Ionic strength effects on metal adsorption, Fourier transform infrared spectroscopy, x-ray absorption spectroscopy x-ray absorption spectroscopy including x-ray absorption near-edge structure and extended x-ray absorption fine-structure spectroscopy were employed to elucidate the adsorption mechanisms. The results suggested that Pb and Cu adsorption on POM was mainly through inner-sphere complexes with carboxyl and hydroxyl groups. Cadmium was possibly adsorbed via outer-sphere complexation, indicated by the influence of ionic strength on Cd adsorption.
Identification of compounds associated with testosterone depressions in fish exposed to bleached kraft pulp and paper mill chemical recovery condensates
Authors: Belknap Andrew M. , Solomon Keith R. , MacLatchy Deborah L. , Dubé Monique G. , Hewitt L. Mark 
Published: August 2006

AbstractIn previous experiments, bleached kraft chemical recovery condensates generated during softwood pulp production have been identified as a primary source of substances causing testosterone depressions in mummichog (Fundulus heteroclitus). Recent toxicity identification evaluations have encountered inconsistencies in the potential of condensates and fractions to reduce steroid concentrations between three repeated exposures. To assess the sources of these inconsistencies, temporal assessments of condensate extractives and analytical methodologies were evaluated. Condensates were collected during a six-month period, extracted by solid-phase extraction (SPE), and analyzed by gas chromatography–mass spectrometry and gas chromatography–flame photometric detection. All unique extractives were cataloged by mass spectra, peak area, and gas chromatographic retention time, and nine were confirmed and quantified against authentic standards. Confirmed components included phenolic guaiacyl–based lignin degradation products, sulfur (S8), three diterpenoids, and a dimethoxy stilbene. Concentrations of confirmed condensate extractives were consistent in all samples collected. Spiking experiments of confirmed extractives revealed substantial losses following high-pressure liquid chromatographic fractionation and three different methods of fraction preparation. In an effort to propose chemical classes associated with biological activity, all unique condensate extractives in previously established bioactive and inactive fractions were classified based on their potential to depress testosterone in mummichog. Of 39 unique components in bioactive SPE extracts of condensates, six were associated with hormonal activity. Mass spectral interpretation indicated hydroxylated diterpenoids, sesquiterpenoids, and a lignin-derived stilbene as classes of chemicals associated with steroid depressions.