bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exogenous Melatonin Strengthens Saline-alkali Stress Tolerance in M9-T337 Seedlings by Initiating a Variety of Physiological and Biochemical Pathways
Authors: Xian Xulin , Zhang zhongxing , Wang Shuangcheng , cheng Jiao , Gao Yanlong , Ma Naiying , Li Cailong , Wang Yanxiu 
Published: March 2024

Abstract
Melatonin (MT) is an important phytohormone that significantly regulates the growth and development of plants. Previous studies confirmed the effectiveness of MT in improving plant stress tolerance. In this study, annual M9-T337 seedlings were selected as subjects and five treatments were applied: conventional control (CK), in which only half the concentration of Hoagland was applied; Saline-alkali stress treatment (SA, 100 mmol·L-1 Saline-alkali solution); melatonin treatment (MT, CK + 200 μmol·L-1 exogenous MT); Saline-alkali + melatonin treatment (MS, SA + 200 μmol·L-1 exogenous MT); and Saline-alkali stress + melatonin + inhibitor treatment (HS, additional 100 μmol·L-1 p-CPA treatment to MS). The results showed that Saline-alkali stress negatively affected the growth of M9-T337 seedlings by reducing photosynthetic capacity, increasing Na+, promoting reactive oxygen species such as H2O2, and changing the osmotic content and antioxidant system. However, the application of exogenous MT effectively alleviated Saline-alkali damage and significantly promoted the growth of M9-T337 seedlings. It significantly increased plant height, diameter, root length, root surface area, volume and activity. Furthermore, MT alleviated osmotic stress by accumulating proline, soluble sugars, soluble proteins and starch. Furthermore, MT improved photosynthetic capacity by delaying chlorophyll degradation and regulating gas exchange parameters as well as fluorescence parameters in leaves. Furthermore, MT improved the Na+/K+ ratio to reduce ion toxicity by upregulating the expression of Na+ transporter genes (MhCAX5, MhCHX15, MhSOS1, and MhALT1) and downregulating the expression of K+ transporter genes (MhSKOR and MhNHX4). In addition, MT can increase antioxidant enzyme activity (SOD, POD, CAT, AAO, APX and MDH) in the ASA-GSH cycle and increase AsA, GSH and GSSG levels to counteract the accumulation of reactive oxygen species (ROS). such as H2O2 and O2-, reducing oxidative damage. Exogenous MT promotes root growth under salt-alkaline stress by increasing root activity and responding synergistically with IAA, GA3 and ZT to salt-alkaline stress. Our results confirm that MT has the potential to alleviate Saline-alkali stress by promoting root growth, increasing biomass accumulation and photosynthetic capacity, strengthening the antioxidant defense system, maintaining ionic balance, the ascorbate-glutathione cycle and the Osmoregulation facilitates and regulates endogenous hormone levels in M9-T337 seedlings.
The effect of pre-treatments on atrazine removal from source water by microbubble ozonation
Authors: Rehman Ratul , Lu Wanmeng , Shi Lifang , Yang Yahong , Pan Li 
Published: March 2024

Abstract

Ozone-based advanced oxidation processes (AOPs) have emerged as promising technologies for water treatment. Recent studies have shown that ozone microbubbles can enhance ozone mass transfer during water treatment, and combining microbubble ozonation with pre-treatment steps can improve the removal of micropollutants from source water. This study evaluated the removal of atrazine from natural water using three different combined processes (chlorine/KMnO4/PAC pre-treatment followed by ozonation). The results indicate that all three combined processes achieved higher atrazine removal rates than pre-treatment alone or ozonation alone. The atrazine removal rate was highest at alkaline pH values, and all three combined processes were affected by oxidant dose and pH. The combination of chlorine pre-treatment with ozonation was the most effective for natural source water, with a removal rate of 59.7% that exceeded the sum of the individual treatments. However, this combined process was affected by water quality, particularly the nature of organic matter, and a relatively high concentration of ammonia nitrogen (greater than 0.5 mg/L) negatively impacted on treatment outcomes. This study highlights the potential for utilizing ozone micro/nanobubbles to enhance ozone mass transfer and provides valuable insights for optimizing the combined application of pre-treatment and ozonation to remove atrazine from natural water sources efficiently.
Macro-micro Analysis of Geometric Relationship for Triangular Mesh and Application of Direct Sliced Contour Generation
Authors: Wen Hao 
Published: March 2024

Abstract
The triangle mesh has become extensively utilized in many different industrial domains due to the quick advancement of computer-aided design and production technology. For the additive manufacturing of the triangular mesh, the slicing procedure is an important process step to generate the sliced contour. However, the simplify model data will expand to redundant data over this computer data processing operation. This paper focus on the geometric relationship of the triangular mesh and concludes several rules for the intersection points and their retrieved vertices. Based on these rules, a direct sliced contour generation method is presented and achieved by some data operations for the classified vertices. Finally, this proposed method is implemented through computer programming and validated using multiple test models. This proposed method can realize the sliced contour generation of the rapid prototyping systems, which avoid the redundant data with the hidden relationship at the same time. And the concluded rules of the geometric relationship can be applied to the related industrial applications.
Prediction of the failure behavior of pseudo-ductile composites using a multi-scale finite element model
Authors: Abdellahi Behzad , Azhari Fatemeh , Nguyen Phu 
Published: March 2024

Abstract
The hybridization technique has recently been used to produce a new genera-tion of composites called pseudo-ductile composites, which have shown higher failure strain compared to conventional composites, minimizing the risks of the occurrence of a catastrophic failure. The pseudo-ductility behavior in these com-posites is obtained by hybridization of fibers with high and low failure strains. In this study, a multi-scale finite element (FE) model incorporating micro and macro-scales is proposed to predict the failure behavior of pseudo-ductile compos-ites. A micro-scale representative volume element (RVE), consisting of randomly distributed fibers, was generated using a Python code. Periodic boundary con-ditions (PBCs) were applied to the RVE generated with a periodic geometry. To account for fiber failure and ply fragmentation, the tensile strength of fibers was distributed based on the Weibull distribution function and a user-defined UMAT subroutine was developed. Tensile loading was then applied to the RVE to simulate the composite’s mechanical behavior. For validation, an RVE was developed based on experimental data from recent research on thin-ply and con-ventional thickness composites. Numerical results were compared to experimental data, demonstrating acceptable agreement. In the final step, following a sequen-tial multi-scale modeling approach, a macro-scale model was constructed based on the outputs of the micro-scale model subjected to tensile and shear loads. The results were compared with experimental data, revealing good agreement. The proposed model allows for the optimization of pseudo-ductile composite structures to achieve a desired set of mechanical properties without the need for conducting extensive experimental material tests.
Effects of HOX family regulator-mediated modification patterns and tumor immunity characteristics on cancer- associated fibroblasts in endometrial cancer
Authors: Yang Jiaolin , Li JinPeng , Yang Yutong , Su HuanCheng , Guo HongRui , Lei jing , Li SuFen , Wang YaLin , Wen KaiTing , Li Xia , Wang Zhe , Zhang SanYuan 
Published: March 2024

Abstract

Endometrial cancer is one of three major malignant tumors in women. The HOX gene regulates tumor development. However, the potential roles of HOX in the development and progression of endometrial cancer, cancer-associated fibroblasts (CAFs), and tumor microenvironment (TME) cell infiltration remain unknown. Here, a new scoring system was developed to quantify individual HOX patterns in uterine corpus endometrial cancer (UCEC). Meanwhile, our research was verified by immunohistochemistry and real-time quantitative PCR (RT-qPCR) and Single-cell RNA sequencing (scRNA-seq). Our study found that patients in the low HOX score group had abundant anti-tumor immune cell infiltration, good tumor differentiation, and better prognoses. Meanwhile, a high HOX score was associated with blockade of immune checkpoints, which enhances the response to immunotherapy. HOX gene was a pivotal factor in the development and progression of endometrial cancer and in the formation of tumor microenvironment diversity and complexity. HOX genes are involved in the regulation of CAF formation, and thus change microenvironments to promote tumor growth, invasion, and metastasis. In sum, HOX scores can be used as a new biomarker for the diagnosis and prediction of UCEC and to develop more effective therapeutic strategies.
Remote Ischaemic Conditioning for Neurological Disorders – A Systematic Review and Narrative Synthesis
Authors: Alhashimi Ali , Kamarova Maharyta , B Sheharyar , Nair K Sivaraman , Wang Tao , Redgrave Jessica , Majid Arshad , Ali Ali N. 
Published: March 2024

Abstract

Introduction: Remote ischaemic conditioning (RIC) refers to the use of controlled transient ischemic and reperfusion cycles, commonly of the upper or lower limb, to mitigate cellular damage from ischaemic injury. Preclinical studies demonstrate that RIC may have a neuroprotective effect and therefore could represent a novel therapeutic option in the management of neurological disorders. The aim of this review is to comprehensively describe the current clinical evidence of RIC in neurological disorders.
Methods: A computerised search of EMBASE and OVID MEDLINE was conducted from 2002 to October 2023, for randomised controlled trials (RCTs) investigating RIC in neurological diseases.
Results: A total of 46 different RCTs in 12 different neurological disorders (n=7544) were included in the analysis. Conditions included acute ischaemic stroke, symptomatic intracranial stenosis and vascular cognitive impairment. The most commonly used RIC protocol parameters in the selected studies were as follows: cuff pressure at 200mmHg (27 trials), 5-minute cycle length (42 trials), 5 cycles of ischaemia and reperfusion (24 trials), and the application to the upper limb unilaterally (23 trials) .
Conclusions: The comprehensive analysis of the included studies reveals promising results regarding the safety and therapeutic effect of RIC as an option for managing neurological diseases. Particularly, the strongest evidence supports its potential use in chronic stroke patients and vascular cognitive impairment. The neuroprotective effects of RIC, as demonstrated in preclinical studies, suggest that this therapeutic approach could extend its benefits to various other diseases affecting the nervous system. However, to establish the efficacy of RIC across different neurological disorders, further trials with larger sample sizes and more diverse patient populations are warranted. Upcoming trials are expected to provide valuable evidence that will not only confirm the efficacy of RIC in neurological disease management but also help identify the most optimal RIC regimen for specific conditions.
Dynamic Interplay Between miR-124-3p and EGF in Regulating The 'Giant' Phenotype by RNA Signalling Mechanism
Authors: BAYRAM Keziban KORKMAZ , Bayram Arslan , Suranli Zeynep Yilmaz , Mehmetbeyoglu Ecmel , Aybuga Fatma , Tufan Esra , Taheri Serpil , Ozkul Yusuf , Rassoulzadegan Minoo 
Published: March 2024

Abstract

Epigenetic mechanisms and RNA signalling profoundly impact body growth during the early stages of embryonic development. Epigenetic modifications' critical role in determining which genes are activated or suppressed, thereby influencing the development. RNA molecules, like microRNAs, regulate gene expression, control cell fate, and coordinate cellular processes crucial for proper growth and development. These complex molecular processes work harmoniously to create the intricate symphony of early embryonic development, which lays the foundation for the organism's future growth and function. Mouse embryos that are microinjected with miR-124-3p (miR-124*) exhibit a significantly 'giant' phenotype before and after birth. Behavioural test results showed that miR-124* mice were more cognitively active. miR-124 is highly expressed in the brain; however, the molecular mechanism leading to the phenotypic changes mediated by miR-124-3p is unknown. This study aimed to investigate the role of epidermal growth factor (EGF) in developing a distinct phenotype ('giant and cognitive') in miR-124* mice. We established neurosphere (NS) culture from the hippocampus at E19.5 using EGF. In miR-124*, Doublecortin (Dcx), Neurod1 (12 days old NS) and Sox8, Sox9, Sox10 (21 days old NS) are significantly overexpressed.
Our results suggest that the addition of EGF likely leads to overexpression of transcription factors such as Sox8, Sox9 and Sox10, while it decreased the expression of neurogenesis marker Dcx and neuronal differentiation marker Neurod1 when the culture period is extended up to 21 days in miR-124* NS. Following the microinjection of miR-124-3p into the embryo at the one-cell stage, EGF could have been activated and suppressed the expression of miR-124-3p, leading to early neurogenesis and neuronal differentiation in embryos. It led to the earlier emergence of mice with cognitively active and 'giant' phenotypes.
Clinical, genetic, and sociodemographic predictors of symptom severity after internet-delivered cognitive behavioural therapy for depression and anxiety
Authors: Kravchenko Olly , Boberg Julia , Mataix-Cols David , Crowley James , Halvorsen Matthew , Sullivan Patrick , Wallert John , Rück Christian 
Published: March 2024

Abstract

Internet-delivered cognitive behavioural therapy (ICBT) is an effective and accessible treatment for mild to moderate depression and anxiety disorders. However, up to 50% of patients do not experience sufficient symptom relief. Identifying patient characteristics predictive of higher post-treatment symptom severity is crucial for devising personalized interventions to avoid treatment failures and reduce healthcare costs. Using the new Swedish multimodal database MULTI-PSYCH, we expand upon established predictors of treatment outcome and assess the added benefit of utilizing polygenic risk scores (PRS) and nationwide register data in a combined sample of 2668 patients treated with ICBT for major depressive disorder (n = 1300), panic disorder (n = 727), and social anxiety disorder (n = 641). We present two linear regression models: a baseline model using six well-established predictors and a full model incorporating six clinic-based, 32 register-based predictors, and PRS for seven psychiatric disorders and traits. First, we assessed predictor importance through bivariate associations and then compared the models based on the proportion of variance explained in post-treatment scores. Our analysis identified several novel predictors of higher post-treatment severity, including comorbid ASD and ADHD, receipt of financial benefits, and prior use of some psychotropic medications. The baseline model explained 27% of the variance in post-treatment symptom scores, while the full model offered a modest improvement, explaining 34%. Developing a machine learning model that can capture complex non-linear associations and interactions between high-quality multimodal input features is a viable next step to improve prediction of symptom severity post ICBT.
Ambient Temperature and its Nonlinear Relationship with the Risk of Oral Disease: A Time-Series Study from Guangzhou
Authors: Tong Xiaojie , Su Heng , Xu Lei , Wu Xiaoxue , Shi Zehai , Liu Chang , Chen Jin 
Published: March 2024

Abstract

Previous studies have noted a distinct seasonal variation in oral diseases, which appeared to align with the patterns of climate change. This observation sparked our interest in investigating whether there is a definitive correlation between ambient temperature fluctuations and the incidence of oral diseases in different climatic cities of China, a connection that, to date, remains unclear. This study aimed to elucidate the relationship between ambient temperature and the incidence of oral diseases. Daily outpatient data from the Affiliated Stomatological Hospital of Guangzhou Medical University spanning 2020 to 2022 were collected, and a time-series analysis was conducted. We fit the data utilizing a generalized additive model and a distributed lag nonlinear model, adjusting for factors such as relative humidity, time trends, day of the week, and holidays. Our evaluation focused on discerning the relationship between ambient temperature and oral diseases. We amassed a total of 206,729 outpatient oral disease patients. The results showed that low temperatures (below 12–13°C) correlated with a greater risk of oral diseases. In contrast, temperatures above 12–13°C appeared to have a negligible influence on oral ailments. Relative to our reference temperature, the cumulative risk factors for various oral diseases at low temperatures (8.9°C, 1st percentile) were as follows: dental caries (2.28; 95% CI: 1.69–3.08), periodontitis (1.92; 95% CI: 1.38–2.67), pulpitis (2.91; 95% CI: 2.0-4.23), periapical periodontitis (3.15; 95% CI: 2.06–4.82) and temporomandibular joint disorder syndrome (TMD) (1.63; 95% CI: 0.89–3.01). These associations were more significant in patients older than 60 years. Our findings revealed a nonlinear correlation between ambient temperature and the risk of oral diseases in Guangzhou. Lower temperatures have become significant risk factors for oral diseases and older patients.
Stevens-Johnson Syndrome Induced by Allopurinol
Authors: Zhu Ying , Li Dandan , Zhang Hong , Li Jiandong , Chang De 
Published: March 2024

Abstract

Background and Objective:
Adverse drug reaction leading to Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis(TEN) is a rare and potentially life-threatening adverse effect of allopurinol. Before allopurinol initiation, screening for patients with HLA-B*58:01 is recommended to avoid the risk of complications.
Case description:
An 85-year-old man was admitted to our hospital after initiating allopurinol for treating hyperuricemia, presenting with diffuse maculopapular rash on entire body. The level of inflammatory indicators such as C-reaction protein(CRP), erythrocyte sedimentation rate (ESR) and procalcitonin and the amount of eosinophilic granulocyte were decreased after discontinuation of allopurinol and corticosteroid administration, while deterioration of the situation occurred after quick glucocorticoid withdrawal. Reinstitution of the glucocoritcoids with immunoglobulin showed clinical improvement. Despite the amelioration of SJS, the patient died after 40 days of hospitalization with the acute coronary syndrome.
Conclusion
A case of allopurinol-induced SJS/TEN syndrome suggests that clinicians should prescribe allopurinol to hyperuricemia patients with caution. Detection of HLA-B*58:01 for allopurinol hypersensitivity among high-risk individuals is warranted. Timely treatment with glucocosteroid and immunoglobulin might be helpful in the management of this rare but serious adverse reaction.
Admission viscoelastic hemostatic assay parameters predict poor long-term intracerebral hemorrhage outcomes
Authors: Sieh Laura , Peasley Emma , Mao Eric , Mitchell Amanda , Heinonen Gregory , Ghoshal Shivani , Agarwal Sachin , Park Soojin , Connolly E. Sander Sander , Claassen Jan , Moore Ernest E. , Hansen Kirk , Hod Eldad A , Francis Richard O. , Roh David 
Published: March 2024

Abstract

Background
Viscoelastic hemostatic assays (VHA) provide more comprehensive assessments of coagulation compared to conventional coagulation assays. While VHAs have enabled guided hemorrhage control therapies, improving clinical outcomes in life-threatening hemorrhage, the role of VHAs in intracerebral hemorrhage (ICH) is unclear. If VHAs can identify coagulation abnormalities relevant for ICH outcomes, this would support the need to investigate the role of VHAs in ICH treatment paradigms. Thus, we investigated whether VHA assessments of coagulation relate to long-term ICH outcomes.
Methods
Spontaneous ICH patients enrolled into a single-center cohort study receiving admission Rotational Thromboelastometry (ROTEM) VHA testing between 2013 and 2020 were assessed. Patients with prior anticoagulant use or coagulopathy on conventional coagulation assays were excluded. Primary ROTEM exposure variables were coagulation kinetics and clot strength assessments. Poor long-term outcome was defined as modified Rankin Scale ≥ 4 at 6 months. Logistic regression analyses assessed associations of ROTEM parameters with clinical outcomes after adjusting for ICH severity and hemoglobin concentration.
Results
Of 44 patients analyzed, mean age was 64, 57% were female, and the median ICH volume was 23 mL. Poor 6-month outcome was seen in 64%. In our multivariable regression models, slower, prolonged coagulation kinetics (adjusted OR for every second increase in clot formation time: 1.04, 95% CI: 1.00–1.09, p = 0.04) and weaker clot strength (adjusted OR for every millimeter increase of maximum clot firmness: 0.84, 95% CI: 0.71–0.99, p = 0.03) were separately associated with poor long-term outcomes.
Conclusions
Slower, prolonged coagulation kinetics and weaker clot strength on admission VHA ROTEM testing, not attributable to anticoagulant use, were associated with poor long-term outcomes after ICH. Further work is needed to clarify the generalizability and the underlying mechanisms of these VHA findings to assess whether VHA guided treatments should be incorporated into ICH care.