bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studies on group‐specific antigens in tumours induced with avian tumour viruses in rats
Authors: Thurzo V. , Šimkovičová M. , Šimkovič D. 
Published: February 2007

AbstractGroup‐specific complement‐fixing antigen (GS antigen) was studied by the complement‐fixation test in various avian and mammalian tumours, induced with the following strains of avian tumour virus (ATV): Schmidt‐Ruppin strain of Rous sarcoma virus (SR‐RSV), Prague strain of RSV (PR‐RSV), Fujinami sarcoma virus (FSV) and Bratislava 77 virus (B77V).All primary tumours induced in chicks by SR‐RSV, FSV and B77V, as well as all duck tumours induced with B77V, contained high titres of GS antigen when studied with high‐titre rabbit antiserum against purified GS antigen of avian myeloblastosis virus (AMV). In three out of six B77V‐induced rat tumours (two virus‐producing and one non‐virogenic) high titres of GS antigen were detected when examined with rabbit antiserum and with some rat sera obtained from tumour‐bearing animals. In three remaining B77V‐induced virogenic rat tumours (C21, C22 and C23), GS antigen was not detected with rabbit antiserum; however, sera obtained from these three tumour‐bearing rats contained high titres of GS antibodies when tested with different GS antigens. Positive results were obtained also with PR‐RSV‐induced virogenic rat tumour XC. However, all attempts to detect GS antigen in one SR‐RSV induced virogenic rat tumour (MRI) gave negative results. Possible heterogeneity of ATV‐induced GS antigen in mammalian tumours is discussed.
Localisation cellulaire d'un antigène embryonnaire de tumeurs coliques humaines
Authors: Kleist Sabine Von , Burtin Pierre 
Published: February 2007

AbstractUne étude microscopique en immunofluorescence a été entreprise pour localiser l'antigène carcino‐embryonnaire (ACE), isolé auparavant de tumeurs coliques humaines. Des coupes à congélation de tissu péritumoral, non tumoral, de muqueuse colique normale et d'intestins fœtaux ont été examinées en utilisant la méthode indirecte de Coons. Des images caractéristiques de fluorescence ont été observées lorsque l'immun‐sérum anti‐antigène carcino‐embryonnaire (ACE) a été employé. Dans les tumeurs et dans l'intestin fœtal, les membranes libres des cellules bordant la lumière des glandes sont devenues fortement fluorescentes révélant ainsi la présence de l'ACE. Quand l'antisérum est absorbé par l'ACE, cette réaction disparaǐt. Des contrǒles avec des antisérums différents ne donnent pas les měmes résultats. Ceci est en faveur de la spécificité des images obtenues.
Culture in vitro d'une lignée cellulaire provenant d'un lymphosarcome humain. I. Cytochimie, cytogénétique et ultrastructure cellulaire
Authors: Suárez Horacio Guillermo , de Salum Sonia Brieux , Pavlovsky Santiago , Ruibal Beatriz , Pavlovsky Alfredo 
Published: February 2007

AbstractLa morphologie, la cytochimie, la cytogénétique et I'ultrastructure d'une lignée transforniée in vitroprovenant d'un lymphosarroine humarn ont été étudiées. Les cellules, en culture depuis plus d'un an, sont des cellules blastiques très primitives et ne présentant aucune ressemblance avec les cellules originales, qui étaient des lymphocytes et lymphoblastes typiques. A I'origine, la population cellulaire en culture se composait rle cellules en suspension de type lymphoïde et de fibroblastes fixés au verre. A partir de la 4e semaine, de grosses cellules rondes adjacentes aux fibroblastes sont apparues. Leur nombre a augmenté progressivement, donnant lieu à la population cellulaire blastomateuse définitive. Les fibroblastes ont disparu aux environs de la 14e semaine après la mise en culture. A partir de ce moment‐là, les cellules ont poussé en suspension sans l'aide d'une couche alimentaire. Du point de vue cytochimique, les cellules étaient fortement PAS‐positives, mais peroxidase‐, phosphatase‐alcaline‐et phosphatase‐acide‐négatives. L'analyse cytogénétique a montré un chiffre modal polyploïde, avec une dispersion irrégulière autour de la valeur diploïde. Les chromosomes surnuméraires appartenaient aux groupes de Denver; ils prédominaient dans les groupes 6–12 + X, 19–20 et 21–22. Un chromosome “marqueur” long acrocentrique était présent dans 90% des mitoses. La microscopie électronique a montré la présence de cellules blastiques indifférenciées avec un cytoplasme riche en organites, des noyaux avec un ou plusieurs grands nucléoles et une chromatine lǎche. Quelques cellules présentaient des particules dont la morphologie et la taille étaient très irrégulières (80–120 μ) et qui peuvent ětre considérées comme des produits de sécrétion métabolique. D'autres cellules montraient des inclusions dans le cytoplasme, les mitochondries et le noyau, qui ne peuvent pas ětre interprétées. Les cellules ne contenaient pas d'antigènes de fixation du complément ou agents cytopathogènes de type Polyome, SV40 et Adénovirus. Cette transformation cellulaire pourrait ětre dépendante d'une complémentation entre les éléments fibroblastiques et lymphoïdes. Il est impossible d'établir actuellement si cette transformation est due à une modification génétique ou bien à l'incorporation d'un agent viral inconnu.
Report on controlled therapeutic trials in progress registered by the information office of the committee on controlled therapeutic trials of the UICC
Authors: Flamant R. 
Published: February 2007

AbstractThe Committee on Controlled Therapeutic Trials (Commission of Clinical Oncology, International Union Against Cancer) has decided to establish an information office. The office collects the basic information on controlled clinical trials, planned or in progress, and distributes the filed information in order to assist investigators in the field. Information is made available either by direct request from the Secretary of the Committee or by periodically published lists. The first list of trials reported to the Office is presented.
Persistance de la synthèse de RNA extranucléolaires dans le foie de rats traités par la lasiocarpine. étude par autoradiographie a haute résolution
Authors: Monneron Ariane , Kerros Nicole 
Published: February 2007

AbstractLa synthèse résiduelle de RNA dans le foie de rats soumis à une injection de lasiocarpine a été étudiée par autoradiographie à haute résolution après administration d'uridine tritiée. Les blocs ont été débarrassés autant que possible des précurseurs acido‐solubles marqués du RNA, et une technique très sensible de développement a été choisie, compte tenu de la forte inhibition de synthèse du RNA chez les animaux traités. Le marquage est très faible au niveau des nucléoles. Il est présent sur le nucléoplasme, en particulier le long de la chromatine compacte et sur les grains interchromatiniens. Les inclusions nucléaires induites par la lasiocarpine sont également marquées. Le fonctionnement de la RNA‐polymérase est donc moins affecté par la lasiocarpine au niveau des sites de transcription extranucléolaire qu'il ne l'est dans les nucléoles. Ces résultats sont rapprochés d'études faites sur des noyaux isolés de rats traités par la lasiocarpine et synthétisant des RNA in vitro.
Présence D'une Ferroprotéine D'origine Tissulaire, L'α2H, dans le Sérum des Enfants Porteurs de Tumeurs
Authors: Buffe D. , Rimbaut C. , Lemerle J. , Schweisguth O. , Burtin P. , Rudant C. H. 
Published: February 2007

AbstractA l'aide d'un antisérum marqué à l'iode 131, nous avons mis en évidence par radio‐immunodiffusion une protéine d'origine tissulaire, l'α2H, dans le sérum de 81% des enfants porteurs de tumeurs. Sur les 122 sérums d'enfants normaux ou atteints d'affections bénignes, 10 seulement étaient positifs soit 8%. L'intérět clinique de ce test est discuté.
Effect of azathioprine on the chromosome complement of human bone marrow cells
Authors: Jensen Mogens Krogh 
Published: February 2007

AbstractChromosome studies were performed on bone marrow aspirates from 15 patients treated with azathioprine. In most patients the immunosuppressive therapy produced structural changes of the chromosome complement. The structural chromosome aberrations disappeared after cessation of therapy. In four patients no evidence of chromosome abnormalities could be found during therapy with azathioprine. It is proposed that the study of bone marrow cells may be more important than that of short‐term leukocyte cultures when estimating the chromosome damaging properties of some chemical agents.
Comparison of the anti‐ebv titer and the ebv‐associated membrane reactive and precipitating antibody levels in the sera of burkitt lymphoma and nasopharyngeal carcinoma patients and controls
Authors: Klein G. , Geering G. , Old L. J. , Henle G. , Henle W. , Clifford P. 
Published: February 2007

AbstractComparisons were made between 151 sera from 131 donors with Burkitt's lymphoma, nasopharyngeal carcinoma and other tumors of the head and neck area, from patients having had infectious mononucleosis and from healthy controls, in three EBV‐associated serological tests: intracellular immunofluorescence on fixed smears of EBV‐carrying lymphoblastoid cell lines (“anti‐EBV‐reaction”), blocking of membrane fluorescence on similar lines and immunoprecipitation against the soluble antigen extracted from the EBV‐carrying P3J culture line. Anti‐EBV and membrane blocking antibody levels showed concordant patterns in 122 sera (81%) and discordant in 29 (19%), in agreement with previous results. If the anti‐EBV and the precipitin tests are compared, 81.5% of the sera gave concordant and 18.5 discordant results. Blocking of membrane reactivity gave concordant results with the precipitin test in 71.5% of the sera whereas 28.5% were discordant. All three tests gave concordant results in 67% of the serum material; this figure fits expectations if it is assumed that the probability of an association between the anti‐EBV titer and the membrane blocking antibody level is independent of the probability of an association between the anti‐EBV titer and the antibody detected by the precipitin test. Analysis of the serological patterns in relation to disease categories shows that the sera of patients with head and neck tumors other than carcinoma of the post‐nasal space or Burkitt's lymphoma fell mainly within the low reactive categories. Sera from nasopharyngeal carcinoma and African Burkitt lymphoma patients showed the opposite behavior, with a majority of highly reactive sera according to all three tests. Most control sera were negative or low in all three tests. In one Burkitt patient, the serological findings could be related to the clinical course of the disease; high membrane reactive antibody levels were seen in the course of tumor regression, but fell to low levels before and immediately after recurrence. Precipitating antibodies showed the opposite pattern, being absent during regression and highly positive after recurrence.
Immunotherapy of primary moloney sarcoma‐virus‐induced tumors
Authors: Fefer A. 
Published: February 2007

AbstractAdult mice inoculated with MSV develop tumors, 90% of which undergo immunologically‐mediated regression. Sublethal cyclophosphamide (CY) administered to such tumor‐bearing mice prevented regression, so that 58/60 mice died with tumors. As hosts for immunotherapy, adult BALB/c mice bearing primary tumors and given CY were injected with BALB/c spleen cells. Cumulatively, 80/85 tumors treated with cells from mice immunized with MSV completely regressed, whereas only 6/92 tumors regressed after treatment with normal or non‐specifically immunized cells, or with non‐viable immune cells. The anti‐tumor effect of CY was not essential for the immunotherapeutic effect of cells. Similar experiments were performed with allogeneic spleen cells. DBA cells were effective only if specifically pre‐immunized with MSV or BALB/c Moloney sarcoma cells. Mice treated with cells from C57BI/6 donors immunized with MSV died tumor‐free with graft‐versus‐host disease, whereas mice given non‐immunized cells died of graft‐versus‐host disease with tumor. Studies with gamma‐globulin allotype as a marker for donor cells suggested that immune cells may have to persist in the host for some time in order to be effective against the tumor. Finally, specific serotherapy was only moderately effective as compared to cellular therapy.
Spleen foci and polycythemia in C57BI mice infected with host‐adapted friend leukemia virus
Authors: Steeves R. A. , Mirand E. A. , Bulba A. , Trudel P. J. 
Published: February 2007

AbstractThe host range of Friend leukemia virus was altered significantly by passage in newborn C57Bl mice. Two virus isolates (BSB and BB6), derived from the original Friend virus stock, induced the familiar “Friend disease” and polycythemia in suckling or adult C57Bl mice. However, both isolates differed from the parent virus in Swiss mice by their longer latent period and lower mortality in adult C57Bl mice. Replication of spleen focus‐forming virus (SFFV) from either isolate was limited in suckling mice and barely detectable in adult C57Bl mice. Host susceptibility to SFFV in the BSB isolate varied according to age in young adult C57Bl mice, and was also affected by a gene closely linked with or at the Histocompatibility‐2 locus. Spleen focus formation by either BSB or BB6 in adult BALB/c mice gave one‐hit dose‐response relationships, in contrast to the multiple‐hit relationship obtained with the parent virus. Thus, infection of C57Bl mice with either of two host‐adapted viruses resulted in the development of polycythemia and splenomegaly without extensive SFFV replication, in contrast to earlier observations with the original Friend virus preparation in Swiss mice. Even with the present virus‐host system, however, there was an association between virus replication and the virulence of the induced disease. In addition, there was an association between adaptation of SFFV to the C57Bl strain and an increased concentration of either helper virus or competent virions. Reasons for the low virus titers in several mouse strains and for the limited virus replication in the two strains tested were not apparent.
Effect of autologous mammary tumour extracts on human leukocyte migration in vitro
Authors: Andersen Vagn , Bjerrum Ole , Bendixen Gunnar , Schiødt Torben , Dissing Inger 
Published: February 2007

AbstractIn eight out of 22 patients with mammary carcinoma, extract of tumour tissue induced inhibition of autologous leukocyte migration in vitro, demonstrating a state of cellular (delayed type) hypersensitivity. Non‐tumorous tissue did not inhibit migration. The effect of the carcinoma extracts was not due to non‐specific toxicity, since the migration of leukocytes from matched controls was not affected by the extracts.In nine cases of benign mammary lesions, extracts of autologous mammary tissue did not induce inhibition of migration.Preliminary results of follow‐up studies in some of the carcinoma patients are reported.
Hemolysin production in spleen and peripheral blood cells from normal and leukemic chicks. I. Quantitative assay
Authors: Lagerlöf Bengt , Sundelin Pär 
Published: February 2007

AbstractCell suspensions from spleen, peripheral blood, thymus and bursa of Fabricius of normal chicks and cells from spleen and peripheral blood of chicks with myeloid leukemia were investigated by the hemolytic plaque technique. Spleen cells from normal chicks older than 14 days produced maximum relative numbers of hemolytic foci 5 days after immunization. No signs of hemolysin production were found in cells from peripheral blood, thymus or bursa of Fabricius from non‐leukemic chicks.Spleen cell suspensions from many chicks with myeloid leukemia produced fewer hemolytic foci than spleen cells from normal chicks. However, in some leukemic chicks the immunological reactivity per spleen was similar to that of non‐leukemic spleens. In these chicks white cells in peripheral blood were also capable of PFU formation.
Hemolysin production in spleen and peripheral blood cells from normal and leukemic chicks. II. Ultrastructural analysis of the plaque‐forming cells
Authors: Lagerlöf Bengt , Sundelin Par 
Published: February 2007

AbstractThe cells of normal and leukemic chicks producing hemolytic plaques in agar after stimulation with SRBC have been examined ultrastructurally. The plaque‐forming cells from non‐leukemic chicks were generally plasma cells. This was also the case for 21 of 30 plaques formed by spleen or peripheral blood cells from chicks with myeloid leukemia, while in the nine remaining plaques the plaque‐forming cells were very immature blast cells.
Effect of metabolic inhibitors on membrane immunofluorescence reactivity of established burkitt lymphoma cell lines
Authors: Yata J. , Klein G. , Hewetson J. , Gergely L. 
Published: February 2007

AbstractThe expression of the EBV‐associated membrane antigen complex was studied in three lines of cultured Burkitt lymphoma (BL) cells after exposure to various metabolic inhibitors and X‐irradiation. Direct membrane immunofluorescence (MIF) with a BL reference conjugate (F‐Mutua) revealed a high percentage of positive cells in two of the cell lines (Maku and Onesmas), provided they were cultured under conditions previously defined as optimal for the expression of the membrane antigen. The third cell line (Silfere) contained less than 5% MIF‐positive cells regardless of the culture conditions. Mitomycin C, actinomycin D, methotrexate and X‐irradiation increased the frequency of positive cells in Maku and Onesmas cultures kept under conditions favoring a low membrane antigen expression. Puromycin decreased the frequency of MIF‐positive cells in highly positive cultures from 50% to less than 10%. The increase or decrease in MIF became apparent 3 days after application of the inhibitor. Cytosine arabinoside and low temperature (31°C and 28°C) had no effect on MIF in cultures with either high or low membrane antigen expression, although cell growth was suppressed. In the Silfere cell line, none of the inhibitors studied had any effect on the expression of the membrane antigens.
Histochemical study on rat liver glycogen during dab carcinogenesis
Authors: Forget A. , Daoust R. 
Published: February 2007

AbstractThe distribution of glycogen in livers of DAB‐fed rats has been examined by the PAS reaction. At early stages of DAB feeding, a loss of glycogen was observed in cells of centrolobular areas undergoing a degenerative process. Intense glycogen staining was shown, on the other hand, by the hyperplastic nodules which develop from cells of periportal areas. At later stages, the formation of hyperbasophilic foci was found to be accompanied by a loss of glycogen, and the hepatomas, which apparently arise from such foci, were PAS‐negative.It thus seems that a radical change in glycogen metabolism is associated with the neoplastic transformation. Alterations inglycogen metabolism being considered irrelevant to neoplasia per se, these results suggest that hyperbasophilic foci are the sites not only of modifications in the regulation of the cell cycle that initiate neoplastic growth but also of parallel changes responsible for secondary features of tumors.
Type‐C particles in human tissues. I. Electron microscopic study of embryonic tissues in vivo and in vitro
Authors: Chandra S. , Liszczak T. , Korol W. , Jensen E. M. 
Published: February 2007

AbstractThirty‐two early‐passage human embryonic cultures were examined in the electron microscope and one of them, derived from a muscle tissue, contained two budding and four mature type‐C virus particles. Of the 15 original embryonic tissues from five embryos, only one showed the presence of a virus‐like particle. It is of interest to note that the tissue specimens which contained virus‐like particles originated from the same embryo.
Type‐C particles in human tissues. II. Electron microscopic study of embryonic cultures infected with a murine leukemia virus
Authors: Chandra S. , Stephens R. , Wright B. S. , Korol W. , Zelljadt I. , Jensen E. M. 
Published: February 2007

AbstractType‐C virus particles are synthesized by cells from a number of human embryonic cultures infected with the Rauscher murine leukemia virus (RV). These particles have the characteristic morphology of the murine leukemia viruses. In some cultures, type‐C particles were also observed budding into the cisternae of rough endoplasmic reticulum of a few cells. Virus particles in the vicinity of such cells were of “immature” type. In contrast, particles in the vicinity of those cells which did not synthesize intracisternal type‐C particle were of the “mature” type. It is not known if the “mature” intracisternal particles have the morphology of the “mature” extracellular type‐C particles.
Recovery from the inhibitory effect of X‐radiation on urethan lung adenomagenesis
Authors: Bartlett Gerald L. 
Published: February 2007

AbstractMale (C2HfBiHe x Alele)F1 mice irradiated with 500 R 24 h prior to urethan treatment developed fewer lung adenomas than non‐irradiated, urethan‐treated mice. When irradiation was given two weeks before irrethan treatment, adenoma incidence was the some as in non‐irradiaetd controls, suggesting recovery fromthe tumorinhibitory effrect of radiation. The recovery was not prevented by thymectomy prior to irradiation.Irradiation of female (C3HfBiHe × AjHe)F1 mice with 500 R did no significantly reduce the numver of adenomas induced by urethan.
The reversibility of inhibition of RNA and DNA synthesis induced by aflatoxin in rat liver. A tentative explanation for carcinogenic mechanism
Authors: Lafarge C. , Frayssinet C. 
Published: February 2007

AbstractAflatoxin is a potent hepatocarcinogen. Biochemically the short‐term action which seems to be the most important is an inhibition of nucleic acid synthesis. Injection of low doses (from 500 to 1,000 μg/kg) made it possible to modulate the different inhibitions induced by aflatoxin.The following events are successively observed: first, inhibition of nucleolar RNA synthesis, then inhibition of replication and, finally, inhibition of total nuclear transcription. With such aflatoxin doses these phenomena are reversible, and the different syntheses recommence in the following order: after 24 h the total nuclear RNA synthesis; next, nucleolar RNA synthesis and lastly, after 48 h, DNA synthesis.Therefore there is a period of at least 24 h during which RNA is synthesized and DNA is not.These results may be compared to those obtained by other authors working in vitro and showing aflatoxin‐DNA binding. They support a hypothesis concerning the carcinogenesis mechanism proceeding by deletion at the genetic level.