bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation of Fel d 1 chain 2 genome-edited cats by CRISPR-Cas9 system
Authors: Lee Sang Ryeul , Lee Kyung-Lim , Song Seok-Hwan , Joo Myeong-Don , Lee Seo-Hyun , Kang Ji-Su , Kang Seon-Min , Idrees Muhammad , Kim Jae-Wook , Kong Il-Keun 
Published: February 2024

AbstractAllergens from domestic cats (Felis catus) cause allergy-related health problems worldwide. Fel d 1 is a major allergen that causes severe allergic reactions in humans, including rhinitis, conjunctivitis, and life-threatening asthma. Therefore, patients with cat allergies anticipate hypoallergenic cats. We successfully generated Fel d 1 chain 2 (CH2) genome-edited cats using the CRISPR-Cas9 system in this study. T7 endonuclease 1 assay and Sanger sequencing were used to confirm the mutation in CH2 genome-edited cats. Fel d 1 level in CH2 genome-edited cats were assessed by enzyme-linked immunosorbent assay (ELISA). Remarkably, ELISA showed that the level of Fel d 1 in the CH2 homozygous genome-edited cat (Name: Alsik) was extremely low compared with that in wild type domestic cats and could be hypoallergenic cats. Additionally, we successfully cloned the CH2 homozygous genome-edited cat using cytoplasm injection clone technology. The cloned CH2 homozygous genome-edited cat was verified using microsatellite analysis. Creating hypoallergenic cats using the CRISPR-Cas9 system is a significant step forward because these cats can safely approach allergic patients.
Usefulness of video laryngoscopy in tracheal intubation at thyroid surgical position for intraoperative neuromonitoring
Authors: Won Dongwook , Lee Jung-Man , Lee Jiwon , Chai Young Jun , Hwang Jin-Young , Kim Tae Kyong , Chang Jee-Eun , Kim Hyerim , Kim Min Jong , Min Seong-Won 
Published: February 2024

AbstractThis observational study aimed to compare the glottic view between video and direct laryngoscopy for tracheal intubation in the surgical position for thyroid surgery with intraoperative neuromonitoring. Patients scheduled for elective thyroid surgery with intraoperative neuromonitoring were enrolled. After the induction of anesthesia, patients were positioned in the thyroid surgical posture with a standard inclined pillow under their head and back. An investigator assessed the glottic view using the percentage of glottic opening (POGO) scale and the modified Cormack–Lehane grade in direct laryngoscopy and then video laryngoscopy sequentially while using the same McGRATH™ MAC video laryngoscope at once, with or without external laryngeal manipulation, at the surgical position. A total of thirty-nine patients were participated in this study. Without external laryngeal manipulation, the POGO scale significantly improved during video laryngoscopy compared to direct laryngoscopy in the thyroid surgical position (60.0 ± 38.2% vs. 22.4 ± 23.8%; mean difference (MD) 37.6%, 95% confidence interval (CI) = [29.1, 46.0], P < 0.001). Additionally, with external laryngeal manipulation, the POGO scale showed a significant improvement during video laryngoscopy compared to direct laryngoscopy (84.6 ± 22.9% vs. 58.0 ± 36.3%; MD 26.7%, 95% CI = [18.4, 35.0] (P < 0.001). The superiority of video laryngoscopy was also observed for the modified Cormack–Lehane grade. In conclusion, video laryngoscopy with the McGRATH™ MAC video laryngoscope, when compared to direct laryngoscopy with it, improved the glottic view during tracheal intubation in the thyroid surgical position. This enhancement may potentially facilitate the proper placement of the electromyography tracheal tube and prevent tube displacement due to positional change for thyroid surgery.
Pathophysiology of Trauma-Induced Coagulopathy
Authors: Schöchl Herbert , Schmitt Felix C.F. , Maegele Marc 
Published: February 2024

AbstractTrauma-induced coagulopathy (TIC) is a complex hemostatic disturbance that can develop early after a major injury. There is no universally accepted definition of TIC. However, TIC primarily refers to the inability to achieve sufficient hemostasis in severely injured trauma patients, resulting in diffuse microvascular and life-threatening bleeding. Endogenous TIC is driven by the combination of hypovolemic shock and substantial tissue injury, resulting in endothelial damage, glycocalyx shedding, upregulated fibrinolysis, fibrinogen depletion, altered thrombin generation, and platelet dysfunction. Exogenous factors such as hypothermia, acidosis, hypokalemia, and dilution due to crystalloid and colloid fluid administration can further exacerbate TIC. Established TIC upon emergency room admission is a prognostic indicator and is strongly associated with poor outcomes. It has been shown that patients with TIC are prone to higher bleeding tendencies, increased requirements for allogeneic blood transfusion, higher complication rates such as multi-organ failure, and an almost fourfold increase in mortality. Thus, early recognition and individualized treatment of TIC is a cornerstone of initial trauma care. However, patients who survive the initial insult switch from hypocoagulability to hypercoagulability, also termed “late TIC,” with a high risk of developing thromboembolic complications.
Characteristics of Emission Non-nulling Pulsars Through Simulation
Authors: Yuen Rai 
Published: February 2024

Abstract
We investigate the population and several properties of radio pulsars whose emission does not null (non-nulling) through simulation of a large pulsar sample. Emission from a pulsar is identified as non-nulling if (i) the emission does not cease across the whole pulse profile, and (ii) the emission is detectable. For (i), we adopt a model for switching in the plasma charge density, and emission persists if the charge density is non-zero. For (ii), we assume that detectable emission originates from source points where it is emitted tangentially to the magnetic field-line and parallel to the line-of-sight. We find that pulsars exhibiting non-nulling emission possess obliquity angles with an average of 42.°5, and almost half the samples maintain a duty cycle between 0.05 and 0.2. Furthermore, the pulsar population is not fixed but dependent on the obliquity angle, with the population peaking at 20°. In addition, three evolutionary phases are identified in the pulsar population as the obliquity angle evolves, with the majority of samples having an obliquity angle between 20° and 65°. Our results also suggest that emission from a pulsar may evolve between nulling and non-nulling during its lifetime.
Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis
Authors: Liu Xiaoguang , Chen Zhen , Yan Yuelong , Zandkarimi Fereshteh , Nie Litong , Li Qidong , Horbath Amber , Olszewski Kellen , Kondiparthi Lavanya , Mao Chao , Lee Hyemin , Zhuang Li , Poyurovsky Masha , Stockwell Brent R , Chen Junjie , Gan Boyi 
Published: February 2024

Abstract
Ferroptosis has been recognized as a unique cell death modality driven by excessive lipid peroxidation and unbalanced cellular metabolism. In this study, we established a protein interaction landscape for ferroptosis pathways through proteomic analyses, and identified choline/ethanolamine phosphotransferase 1 (CEPT1) as a lysophosphatidylcholine acyltransferase 3 (LPCAT3)-interacting protein that regulates LPCAT3 protein stability. In contrast to its known role in promoting phospholipid synthesis, we showed that CEPT1 suppresses ferroptosis potentially by interacting with phospholipases and breaking down certain pro-ferroptotic polyunsaturated fatty acid (PUFA)-containing phospholipids. Together, our study reveals a previously unrecognized role of CEPT1 in suppressing ferroptosis.
Effects of Adjunctive Brexpiprazole on Individual Depressive Symptoms and Functioning in Patients With Major Depressive Disorder and Anxious Distress
Authors: McIntyre Roger S. , Bubolic Suzy , Zhang Zhen , MacKenzie Erin M. , Therrien François , Miguelez Maia , Boucher Matthieu 
Published: February 2024

Abstract

Purpose/Background
Anxiety symptoms in major depressive disorder (MDD) are frequent, and they decrease response to antidepressant treatment (ADT), and affect patient functioning. This post hoc analysis examined the efficacy of adjunctive brexpiprazole on individual depressive symptoms and functioning in patients with MDD with anxious distress.

Methods/Procedures
Data were included from three 6-week, randomized, double-blind, placebo-controlled studies of adjunctive brexpiprazole in patients with MDD and inadequate response to ADTs (ClinicalTrials.gov identifiers: NCT01360645, NCT01360632, NCT02196506). Patients were stratified using proxy criteria for Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, anxious distress. Changes in Montgomery-Åsberg Depression Rating Scale item scores and Sheehan Disability Scale mean score from baseline to week 6 were determined for ADT + brexpiprazole (2 and 2–3 mg) versus ADT + placebo.

Findings/Results
At baseline, 450 of 746 patients (60.3%, 2 mg analysis) and 670 of 1162 patients (57.7%, 2–3 mg analysis) had anxious distress. In patients with anxious distress, ADT + brexpiprazole 2 mg or 2 to 3 mg showed greater improvements than ADT + placebo (P < 0.05) on the Montgomery-Åsberg Depression Rating Scale items of apparent sadness, reported sadness, inner tension, reduced sleep, reduced appetite, lassitude, inability to feel, and pessimistic thoughts (Cohen d effect sizes, 0.18–0.44), and on Sheehan Disability Scale mean score (effect sizes, 0.21–0.23).

Implications/Conclusions
Adjunctive brexpiprazole is efficacious in reducing core depressive symptoms, sleep, and appetite, as well as improving functioning, in patients with MDD and anxious distress who have inadequate response to ADTs.
Exploring the determinants of maternal and infant health knowledge adoption, sharing and purchase in short videos from an empathy theory perspective
Authors: Huo Fanfan , Huo Chaoguang 
Published: February 2024

PurposeThis paper aims to explore the determinants of maternal and infant health knowledge (M&IHK) adoption and sharing in the short video from an empathy theory perspective. We explore how to transfer users from free health knowledge to health-related product purchase intention, which is vital for platform knowledge management and service.Design/methodology/approachFocusing on the M&IHK, this study proposes four processes of health knowledge adoption and sharing – knowledge quality persuasion process; source credibility persuasion process; affective empathy emotion process; and cognitive empathy emotion process – to build a framework of M&IHK adoption and sharing. Furthermore, based on adoption and sharing, we explore whether they can promote health-related product purchase intentions. A theoretical model is constructed and tested via Smart PLS in 388 samples.FindingsIn a short video context, perceived knowledge quality and perceived source credibility are still two determinants of health knowledge adoption and sharing. On the contrary, perceived affective empathy and perceived cognitive empathy are two new determinants of health knowledge adoption, but not of health knowledge sharing. Adoption of M&IHK is more driven by both rational thinking and emotional thinking than sharing-only driven by emotional thinking. Adoption and sharing both contribute to health-related product purchase intention, but the female’s intention is more related to rational adoption than the male, which is only related to emotional sharing.Originality/valueThis paper is arguably the first study to examine how short videos impact the mechanisms of M&IHK adoption, sharing and health-related products' purchase intention. It’s perhaps the first study to integrate empathy theory into health knowledge management.
Representing, Re‐presenting, or Producing the Past? Memory Work amongst Museum Employees
Authors: Aroles Jeremy , Morrell Kevin , Granter Edward , Liang Yin 
Published: February 2024

AbstractThough it is widely understood that the past can be an important resource for organizations, less is known about the micro‐level skills and choices that help to materialize different representations of the past. We understand these micro‐level skills and choices as a practice: ‘memory work’ – a banner term gathering various activities that provide the scaffolding for a shared past. Seeking to learn from a context where memory work is central, we share insights from a quasi‐longitudinal study of UK museum employees. We theorize three ideal‐typic regimes of memory work, namely representing, re‐presenting and producing the past, and detail the micro‐practices through which these regimes are enacted. Through explaining the key features of memory work in this context, our paper offers novel, broader insights into the relationship between occupations and memory work, showing how occupations differ in their understanding of memory and how this shapes their memory work.
Non-indicated vitamin B12- and D-testing among Dutch hospital clinicians: a cross-sectional analysis in data registries
Authors: Müskens Joris LJM , Kool Rudolf Bertijn , Westert Gert P , Zaal Maarten , Muller Hein , Atsma Femke , van Dulmen S A 
Published: February 2024

ObjectivesTo assess the extent of non-indicated vitamin B12- and D-testing among Dutch clinicians and its variation among hospitals.DesignCross-sectional study using registration data from 2015 to 2019.ParticipantsPatients aged between 18 and 70 years who received a vitamin B12- or D-test.Primary and secondary outcome measuresThe proportion of non-indicated vitamin B12- and D-testing among Dutch clinicians and its variation between hospitals (n=68) over 2015–2019.ResultsBetween 2015 and 2019, at least 79.0% of all vitamin B12-tests and 82.0% of vitamin D-tests lacked a clear indication. The number of vitamin B12-tests increased by 2.0% over the examined period, while the number of D-tests increased by 12.2%. The proportion of the unexplained variation in non-indicated vitamin B12- and D-tests that can be ascribed to differences between hospitals remained low. Intraclass correlation coefficients ranged between 0.072 and 0.085 and 0.081 and 0.096 for non-indicated vitamin B12- and D-tests, respectively. The included casemix variables patient age, gender, socioeconomic status and hospital size only accounted for a small part of the unexplained variation in non-indicated testing. Additionally, a significant correlation was observed in non-indicated vitamin B12- and D-testing among the included hospitals.ConclusionHospital clinicians order vitamin B12- and D-tests without a clear indication on a large scale. Only a small proportion of the unexplained variation could be attributed to differences between hospitals.
Effects of vitamin D levels during pregnancy on prematurity: a systematic review protocol
Authors: Barbosa Olívia , Sim-Sim Margarida , Silvestre Marta Pereira , Pedro Cristina , Cruz Dulce 
Published: February 2024

IntroductionPrematurity is an urgent public health problem worldwide. Recent studies associate maternal hypovitaminosis D during pregnancy with an increased risk of prematurity. However, the evidence on this association remains inconclusive, and there is lack of consensus in the literature. The exact mechanism by which low vitamin D levels may increase the risk of preterm birth is not yet fully understood. Nevertheless, it is known that vitamin D may play a role in maintaining a healthy pregnancy by regulating inflammation and immunomodulation by acting on the maternal and fetal immune systems. Inflammation and immune dysregulation are both associated with preterm birth, and low vitamin D levels may exacerbate these processes. The results of this review may have important implications for clinical practice and public health policy, particularly regarding vitamin D supplementation during pregnancy.Methods and analysisA systematic review of the literature will be conducted. The search will be performed in electronic databases: CINAHL; MEDLINE; Cochrane Central Register of Controlled Trials; Cochrane Library; Academic Search Complete; Information Science and Technology Abstracts; MedicLatina; SCOPUS; PubMed; and Google Scholar, with the chronological range of January 2018 to November 2022. The search strategy will include the following Medical Subject Headings or similar terms: ‘Vitamin D’; ‘25-hydroxyvitamin D’; ‘Hypovitaminosis D’; ‘Pregnancy’; ‘Pregnant women’; ‘Expectant mother’; ‘Prematurity’; ‘Premature birth’; ‘Premature delivery’; ‘Preterm birth’; and ‘Preterm labour’. This review will include quantitative primary studies, both experimental (clinical trials) and observational (cohort, cross-sectional, and case–control). The quality of each selected study and the results obtained will be assessed by two reviewers separately, using the Cochrane risk of bias tool for evaluating randomised clinical trials or the Newcastle Ottawa Scale for non-randomised studies, following the respective checklist. In case of disagreement, a third reviewer will be consulted.Ethics and disseminationThis study does not involve human subjects and therefore does not require ethics approval. The results will be disseminated through publication in a peer-reviewed scientific journal and through conference presentations. All changes made to the protocol will be registered in PROSPERO, with information on the nature and justification for the changes made.PROSPERO registration numberCRD42022303901.
Experience-based Investigation and Co-design of Psychosis Centred Integrated Care Services for Ethnically Diverse People with Multimorbidity (CoPICS): study protocol
Authors: Bhui Kamaldeep , Joseph Doreen , Khan Nimra , Morrey Tara , Mooney Roisin , Zahid Uzma , Mackay Tanya , Larkin Michael , Keating Frank , McCrone Paul , Upthegrove Rachel , Griffiths Sian Lowri , Edge Dawn , Coventry Peter A , Arday Jason , Hosang Georgina M 
Published: February 2024

IntroductionEthnic minorities (also called racialised groups) are more likely to experience severe mental illness (SMI). People with SMI are more likely to experience multimorbidity (MM), making psychosis among racialised groups more likely to lead to MM, poor outcomes, disability and premature mortality.Methods and analysisThis National Institute for Health and Care Research-funded study (151887) seeks to use innovative participatory methods including photovoice and biographical narrative interviews in urban and rural areas of England to assemble experience data. These data will be subjected to polytextual thematic analysis, and alongside pictures and captions, will inform an experienced-based co-design of interventions, the implementation of which will be evaluated. There will be an economic analysis and a process evaluation of the implementation.Ethics and disseminationThis programme of work has received ethical (IRAS 322421; Newcastle North Tyneside Research Ethics Committee 23/NE/0143) and sponsor approval. The findings will be disseminated in galleries showing the creative work, as lay and academic summaries and infographics; as practice briefings for practitioners, commissioners and policy makers; peer-reviewed publications.Trial registration numberhttps://www.researchregistry.com/browse-the-registry%23home/registrationdetails/649c08111c037d0027b17d17/
Teacher-Mediated Pronunciation Instruction: Its Effects on Enhancing the Oral Language Fluency of Seventh-Grade English Students
Authors: Barrun Jason S. , Sia Jonald B. 
Published: February 2024

This study investigated the effects of teacher-mediated pronunciation instruction on enhancing the oral language fluency of seventh-grade English students. Using a quasi-experimental pretest-posttest non-equivalent groups design, 32 learners served as respondents of the thirteen-session- study in a secondary school in Southern Luzon, Philippines. The student-respondents were tested to find out whether there would be a significant difference in their knowledge and application of the segmentals and suprasegmentals before and after the implementation of the said intervention.
In all the instruments, namely the dictation test, pronunciation achievement test, and aural-oral achievement test, the findings revealed that the experimental group performed significantly higher than the control group. Through the independent sample t-test and Analysis of Covariance (ANCOVA), the results revealed that the experimental group had higher mean scores than the control group with t-computed values exceeding the tabular values affirming that an explicit pronunciation instruction is better in enhancing the oral language fluency of students than only incidental acquisition. The more the learners are exposed to an intervention, the more that they will become intelligible. Other effects also include the more frequently that the teacher conducts various games, the more that the students enjoy and learn pronunciation; and the more refined a learner’s pronunciation skill is, the more improved he/she is when it comes to listening comprehension and spelling. Finally, it is recommended that more and newer interventions regarding second language phonology or any content that focuses on speaking as a macro-strand in English should be delved into.
Stakeholder assessment for mycotoxin analysis: exploring the demand along the European food supply chain
Authors: Csenki E. , Mikulás V. , Freitag S. , Fomina P. , Hlavatsch M. , Femenias A. , Bosman A.J. , Ruggeri F.S. , Smirnova M. , Salentijn G. , Mizaikoff B. , Krska R. , Scholderer J. , Kohler A. , Kasza G. 
Published: February 2024

Abstract
Mycotoxins are amongst the most prevalent food contaminants leading to serious health implications for humans and animals. Limiting exposure to them within the population remains a pressing food safety challenge. Prevention and timely detection are crucial for minimising mycotoxin contamination in food and feed. Therefore easy-to-use, rapid, eco-friendly and low-cost screening methods are increasingly implemented. Early-stage end-user engagement in the technological development process aids and guides the research towards increased societal impact. To investigate these end-user needs, the limitations of the currently used methods and the expectations towards a novel approach were mapped among stakeholders in a comprehensive survey. Stakeholders along the food supply chain (agricultural, food processing, retail, food safety and control, laboratories) were identified in five European countries and contacted. A total of 63 participants completed the survey, which was followed-up by an interview. The results of the survey revealed that different end-user groups have different priorities. Important limiting factors for agricultural, processing and retail stakeholders of the current methodologies include the complexity of sample preparation, high cost and time-to-results. Complementing the accredited laboratory tests with a pre-screening device would be especially interesting for agricultural producers (87.5% of the participants are interested) and food processors (80%), since there is an increasing demand for on-site detection of contamination. On the other hand, food control authorities and commercial laboratories indicated higher priority towards low quantification limits and multi-compound methods. The time to get the results was found to be more important than the testing cost (important aspect for 74.6 versus 66.7%). Overall, the findings of this study are critical input for end-user-targeted development of novel mycotoxin detection platforms.