bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo bioactivity of DNA‐based coatings: An experimental study in rats
Authors: Schouten Corinne , van den Beucken Jeroen J. J. P. , Meijer Gert J. , Sommerdijk Nico A. J. M. , Spauwen Paul H. M. , Jansen John A. 
Published: March 2009

AbstractDNA‐based coatings possess beneficial properties useful for medical and dental implants. The present study evaluated the potential in vivo bioactivity of DNA‐based coatings, either or not pretreated in simulated body fluid (SBF). DNA‐based coatings were generated on titanium cylinders using layer‐by‐layer deposition, with bis‐ureido‐surfactant as the cationic component and DNA as the anionic component. Noncoated titanium implants and CaP‐coated implants served as controls. A total of 80 implants, divided in four experimental groups (n = 10) were implanted unilaterally into the lateral femoral condyles of 80 rats. After implantation periods of 1 and 4 weeks, the bone‐to‐implant contact and bone volume around the implants were determined histomorphometrically. The results of this study showed that DNA‐based coatings and CaP coatings increased bone‐to‐implant contact after 1 week compared to noncoated controls. After 4 weeks of implantation, bone‐to‐implant contact increased significantly for SBF pretreated DNA coatings and CaP coatings, whereas DNA‐coated implants showed no additional effect. The bone‐to‐implant contact of noncoated controls increased to the level of the DNA‐coated implants. Consequently, this study demonstrates that DNA‐based coatings are histocompatible and favor early bone responses. SBF‐pretreated DNA‐based coatings were found to increase both early and late peri‐implant bone responses. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Red cell exchange transfusion for babesiosis in Rhode Island
Authors: Spaete Joshua , Patrozou Eleni , Rich Josiah D. , Sweeney Joseph D. 
Published: March 2009

AbstractWe report four cases of clinically severe tick borne babesiosis treated with chemotherapy and adjunctive red cell exchange (RCE) at two Rhode Island hospitals from 2004 to 2007. All RCE procedures were performed using a Cobe Spectra device and were well tolerated without complications. The volume of allogeneic red cells used in the exchange was determined using the algorithm in the apheresis device with the input variables of preprocedure hematocrit, weight, height, an assumed allogeneic red cell hematocrit of 55 and a desired post procedure hematocrit of 27. The preprocedure level of parasitemia varied between 2.4% and 24% and the postprocedure level of parasitemia between 0.4 and 5.5% with an average overall percent reduction in parasitemia of 74%. Retrospectively, application of a new formula to calculate red cell mass appeared to correlate better with the percent reduction in parasitemia. Previous reports of RCE in babesiosis were reviewed. The reported reduction in parasitemia varied from 50% to >90%. Although a preprocedure level of parasitemia of 10% is sometimes used as a threshold for RCE in clinically severe babesiosis, this threshold does not have a firm empirical basis. No postprocedure desired level of parasitemia is indicated nor the mass of allogeneic red cells needed to achieve such a level. We conclude that current estimates of the dose of allogeneic red cells used in RCE are probably inaccurate, advocate a new formula to estimate this dose and suggest that a 90% reduction in parasitemia should be the minimally desired target of RCE in babesiosis. J. Clin. Apheresis, 2009. © 2009 Wiley‐Liss, Inc.
A hypoxic inducible factor‐1α hybrid enhances collateral development and reduces vascular leakage in diabetic rats
Authors: Kajiwara Hidetoshi , Luo Zhengyu , Belanger Adam J. , Urabe Akihiro , Vincent Karen A. , Akita Geoffrey Y. , Cheng Seng H. , Mochizuki Seibu , Gregory Richard J. , Jiang Canwen 
Published: March 2009

AbstractBackgroundDiabetes mellitus is a common comorbidity of atherosclerosis. Hypoxia‐inducible factor‐1 (HIF‐1) is the master regulator of the angiogenic response to hypoxia.MethodsWe studied the effects of adenoviral vectors expressing a constitutively active HIF‐1α hybrid (Ad2/HIF‐1α/VP16) or vascular endothelial growth factor (Ad2/VEGF) on collateral development and vascular leakiness in a diabetic rat model of hindlimb ischemia.ResultsAfter the removal of the right femoral artery, the mRNA levels of VEGF, angiopoietin‐1 and angiopietin‐4 in the calf muscles, as measured by Taqman reverse transcriptase‐polymerase chain reaction, were transiently elevated in Zucker lean (ZL) but not Zucker diabetic fatty (ZDF) rats. The angiographic score, as determined by post‐mortem angiography, was significantly lower in ZDF animals 35 days after surgery compared to their ZL counterparts. In separate animals, intramuscular injection of Ad2/HIF‐1a/VP16 and Ad/2VEGF into the thigh muscles significantly increased the angiographic score and capillary density 21 and 35 days after the injection compared to Ad2/CMVEV (a vector expressing no transgene) or vehicle. After the injection of Ad2/CMVEV or vehicle, the Evans‐blue dye content in the thigh muscles was significantly higher in ZDF rats than their ZL counterparts. Ad2/HIF‐1α/VP16 but not Ad2/VEGF reduced tissue Evans blue dye content.ConclusionsThe endogenous angiogenic response to ischemia was impaired in ZDF rats, possibly due to down‐regulation of angiogenic factors. Ad2/HIF‐1α/VP16 enhanced collateral development and reduced vascular leakage in the ischemic hindlimb of ZDF rats indicating that hybrid HIF‐1α angiogenic therapy may be efficacious for peripheral vascular disease with a diabetic comorbidity. Copyright © 2009 John Wiley & Sons, Ltd.
Reactions of aromatic ketones with 3‐mercapto‐1,2‐propanediol. Synthesis of cis‐ and trans‐2‐alkyl‐2‐aryl‐(1,3‐oxathiolane‐5‐methanols and 1,3‐dioxolane‐4‐methanethiols)
Authors: Upadhyaya Subhash , Bauer Ludwig 
Published: March 2009

AbstractAromatic ketones react with 3‐mercapto‐1,2‐propanediol (1) in refluxing benzene under the catalytic influence of a sulfonic acid and with azeotropic removal of water to yield a mixture comprised predominantly of cis‐ and trans‐2‐alkyl‐2‐aryl‐1,3‐oxathiolane‐5‐methanols 7, accompanied by lesser amounts of cis‐ and trans‐2‐alkyl‐2‐aryl‐1,3‐dioxolane‐4‐methanethiols 8 (up to 30%). It was discovered that 8 is the kinetic product and is isomerized by 4‐toluenesulfonic acid in hot benzene to the thermodynamically more stable 7. Under these conditions, ortho‐ and α‐substituted aromatic ketones tend to produce more of 8, which can be attributed to steric hindrance encountered by the thiol as it attacks the ketone. Ketalizations of 1‐aryl‐2‐(1H‐imidazol‐1‐yl)‐1‐ as well as 1‐aryl‐2‐(1,H‐1,2,4‐triazol‐1‐yl)‐1‐ethanones by 1 fail under these conditions, even after 24 hours of reflux in toluene. However, 1‐(4‐chlorophenyl)‐3‐(1H‐imidazol‐1‐yl)‐1‐propanone and 1‐(4‐bromophenyl)‐4‐(1H‐imidazol‐1‐yl)‐1‐butanone are ketalized by 1 as expected. Interestingly, the reaction of 2‐bromo‐4′‐chloroace‐tophenone with 1 produces 1‐(4‐chlorophenyl)‐2,8‐dioxa‐6‐thiabicyclo[3.2.1]octane. Characterization of all isomers and separation of some diastereomers is described. Nuclear Overhauser enhancement experiments are utilized to establish the stereochemistry of 1,3‐oxathiolanes.
Synthesis of 1‐[3‐methyl‐2(3H)‐benzazolon‐5‐ or 6‐yl]‐4‐{4‐[cis‐2‐(2,4‐dichlorophenyl)‐2‐(1H‐imidazol‐1‐yl‐methyl)‐1,3‐dioxolan‐4‐yl]methyleneoxyphenyl}piperazines
Authors: Abdelaal Salma M. , Kong Suk‐Bin , Bauer Ludwig 
Published: March 2009

AbstractReactions of 3‐methyl‐6‐[4‐(4‐hydroxyphenyl)‐1‐piperazinyl]‐2(3H)‐benzoxazolone, 3‐methyl‐6‐[4‐(4‐hydroxy‐phenyl)‐1‐piperazinyl]‐2(3H)‐benzothiazolone and 1,3‐dimethyl‐5‐[4‐(4‐hydroxyphenyl)‐1‐piperazinyl]‐2(3H)‐benzimidazolone with cis‐{[2‐(2,4‐dichlorophenyl) ‐2‐(1H‐imidazol‐1‐ylmethyl)]‐1,3‐dioxolan‐4‐yl}methyl meth‐anesulfonate in the presence of sodium hydride furnish the title compounds.
Studies of the reaction of 3‐bromo‐2,5‐dimethylthiophene 1,1‐dioxide with some organocopper reagents
Authors: Gronowitz Salo , Bugarcic Zorica , Hörnfeldt Anna‐Britta 
Published: March 2009

AbstractThe coupling reactions of some organocopper reagents with 3‐bromo‐2,5‐dimethylthiophene 1,1‐dioxide leading to 3‐alkyl and 3‐aryl substituted 2,5‐dimethylthiophene 1,1‐dioxides have been found to proceed in high yields (50–100%). The less stable organocopper reagents reacted faster and at lower temperatures than the more stable ones. It is more convenient to use alkylcopper than lithium dialkylcuprate reagents, which give higher yields without side reactions.
Cyclic transformations of 5‐aryl(or benzyl)‐3‐(2‐bromoethyl‐ 1,3,4‐oxadiazol‐2(3H)‐ones into 1‐aminoimidazolidin‐2‐one and 5,6‐dihydro‐4H‐1,3,4‐oxadiazine derivatives
Authors: Milcent René , Barbier Géo 
Published: March 2009

Abstract5‐Aryl(or benzyl)‐3‐(2‐bromoethyl)‐1,3,4‐oxadiazol‐2(3H)‐ones 3 have been prepared. They were reacted with secondary alkylamines without any change of the heterocycle to give amino derivatives 6, but with primary alkylamines, cyclic transformation occurred to give 1‐acylamino‐3‐alkylimidazolidin‐2‐ones 7. In the presence of sodium alcoholate, bromo compounds 3 were transformed into 2‐aryl(or benzyl)‐4‐alkoxycarbonyl‐5,6‐dihydro‐4H‐1,3,4‐oxadiazines 9.
Selective mono, bis, and trisiminoformylation of 2,4,6‐trialkyl‐1,3,5‐triazines ‐ unusual enol acylenamine tautomerism of hydroxyalkenyl‐1,3,5‐triazines
Authors: Schulz Hans‐Joachim , Liebscher Jürgen , Luger Peter , Quian Minxie , Mulzer Johann 
Published: March 2009

Abstract2,4,6‐Trialkyl‐1,3,5‐triazines 1 can selectively be mono, bis or trisiminoformylated by reaction with trismorpholinomethane 2 or chloromethylene iminium salts 3 (Vilsmeier reagents), with each alkyl substituent reacting with only one molecule of the electrophile. The resulting aminoalkenyltriazines 4, 5 and 6 are partially or fully hydrolysed to corresponding formylation products. The latter exist either in enol or in unusual acylenamine tautomeric structures 10, 12, and 13, depending on the number of enamine or enol units found in the molecule. Reaction of triazines 1 with chlorobenzylidene iminium salts 16 gives monoiminobenzoylation to enaminotriazines 17.
Reissert compound studies. LXV . Preparation of reissert compounds derived from α,β‐unsaturated acid chlorides
Authors: Hahn Jung‐Tai , Kant Joydeep , Popp Frank D. , Chhabra Siri Ram , Uff Barrie C. 
Published: March 2009

AbstractReissert compounds derived from α,β‐unsaturated acid chlorides were prepared. The conjugate base obtained from these Reissert compounds exhibited the following carbanion reactions: 1) Alkylation, 2) Condensation with benzaldehyde, 3) Rearrangement to give dimeric compounds rather than simple rearranged compounds. In the case of alkylated isoquinoline Reissert compounds, the attempted rearrangement led to ring annellated amines.
Synthesis of some benzo[b][1,8] and [1,5]naphthyridines
Authors: Chen Qingping , Deady Leslie W. 
Published: March 2009

AbstractA series of carboxylic acid derivatives of the title compounds have been prepared as precursors of potential anti‐tumor compounds, by annulation of 2‐methylcyclohexanone to the appropriate aminopyridinecarboxaldehyde followed by aromatization and oxidation of the methyl group.
The preparation of perfluoroaryl substituted isoxazoles via nucleophilic aromatic substitution with lithioalkylisoxazoles
Authors: Xia Xiongbing , Knerr Gary , Natale N. R. 
Published: March 2009

AbstractThe lithioalkylisoxazoles obtained from C‐5 lateral metalation of alkylisoxazoles, 1, add to hexafluorobenzene. When the C‐4 substituent was an electron withdrawing tertiary amide moiety, the yields were highest for mono‐perfluoroarylation to give 5‐pentafluorophenylmethylisoxazoles, 2.
Synthesis of 6‐halo‐5‐nitroquinoxalines
Authors: Tian Wei , Grivas Spiros 
Published: March 2009

AbstractThe 5‐nitro derivatives of 6‐haloquinoxalines have been efficiently synthesized by condensation of α‐dicarbonyls with 4‐bromo‐ or 4‐chloro‐3‐nitro‐1,2‐benzenediamines. The novel diamines were readily obtained by reductive cleavage of 5‐bromo‐ and 5‐chloro‐4‐nitro‐2,1,3‐benzoselenadiazoles. As demonstrated by the synthesis of an imidazo‐, a selenadiazolo‐ and a pyrazinoquinoxaline, the reactive halogen atom ortho to the nitro substituent renders the novel quinoxalines versatile intermediates to further heterocycles.
Ring‐closure reaction of N‐arylthiomethylaroylamides to 1,3‐benzothiazines
Authors: Szabó János , Bani‐Akoto Ernest , Dombi György , Günther Gábor , Bernáth Gábor , Fodor Lajos 
Published: March 2009

AbstractN‐Arylthiomethylaroylamides substituted with an electron‐donating group in the meta position undergo two‐directional cyclization in the presence of phosphorus oxychloride to give both positional isomers of the 4H‐1,3‐benzothiazine derivative. The structures of the products were confirmed by 1H and 13C nmr spectroscopy. Mixed ring‐closure reactions of several N‐arylthiomethylaroylamides 3, 6, 9, 13 have shown that these conversions are introduced by a proton‐catalyzed intermolecular rearrangement.
The structures of 2‐(4,5‐dihydro‐5‐aryl‐3‐isoxazolyl)‐1‐arylethanone oximes. An unusual behaviour in its 1H‐NMR and mass spectra
Authors: Díaz Eduardo , Barrios Héctor , Toscano Rubén A. , Yuste Francisco , Reynolds William F. , Aguilera José Luis , Caballero Efrén 
Published: March 2009

AbstractThe reaction of dibenzalacetones substituted on the aromatic ring with hydroxylamine hydrochloride and potassium hydroxide in refluxing ethanol affords several compounds. Herein, we report the structures and spectral properties of several oximes of 2‐(4,5‐dihydro‐5‐aryl‐3‐isoxazolyl)‐1‐arylethanones 6. Unusual behaviour on its 1H‐nmr and mass spectra was observed. Single crystal X‐ray diffraction was performed on compound 6d to support our structural considerations abouth the title compounds.
Synthesis of 3‐substituted‐1‐methylpyrazoles
Authors: Pavlik James W. , Kurzweil Edyth M. 
Published: March 2009

AbstractReaction of 3‐bromo‐1‐methylpyrazole 1 with t‐butyllithium at − 100° followed by quenching of the lithiopyrazole intermediate allows regiospecific introduction of substituents into the 3‐position of the 1‐methylpyrazole ring.
High‐resolution stimulated Raman spectroscopy and analysis of the 2ν10 overtone symmetric motion of C2H4
Authors: Loroño M. , Bermejo D. , Rotger M. , Boudon V. 
Published: March 2009

AbstractThe high‐resolution stimulated Raman spectrum of the 2ν10 band located at 1664.16 cm−1 of C2H4 has been reanalyzed, thanks to the tensorial formalism developed in Dijon for X2Y4 asymmetric‐top molecules. A total of 191 lines were assigned and fitted as a single band without including perturbations such as Fermi or Coriolis coupling constants. We obtained a global root mean square deviation of 8.5 × 10−3 cm−1. Further investigations are required to include interactions with the ν2 and ν7 + ν10 bands. Copyright © 2009 John Wiley & Sons, Ltd.
Polymeric Nanosprings by Bicomponent Electrospinning
Authors: Chen Shuiliang , Hou Haoqing , Hu Ping , Wendorff Joachim H. , Greiner Andreas , Agarwal Seema 
Published: March 2009

AbstractAn enhanced elasticity of electrospun fibers while retaining their strength would be of great benefit for a set of applications (including tissue engineering). One route toward enhanced elasticity may be based on the introduction of curvature, i.e., of buckled or tight nanocoil (nanospring) configurations. The concept investigated in this contribution is to introduce such configurations via a differential shrinkage of a parallel arrangement of a flexible thermoplastic elastomer (polyurethane) component and a rigid thermoplastic component. Core‐shell fibers are produced for this purpose by coaxial electrospinning. It is observed that buckling‐nanospring formation can be achieved in this way. The morphology of such fibers and also modifications in the conductivity of the spinning solutions have a considerable effect on details of buckling and nanospring formation.magnified image
Sol MWD During Styrene, Vinyl Acetate, Methyl Methacrylate, and Butyl Acrylate Homopolymerization: A Numerical Study Using the NFT Approach
Authors: Yáñez‐Martínez Bibiana Alejandra , Luna‐Bárcenas Gabriel , Vivaldo‐Lima Eduardo 
Published: March 2009

AbstractComplete parameter sensitivity analyses using the numerical fractionation technique are presented for the cases of homopolymerization with chain transfer to polymer and termination by combination. Also, using reported values for the kinetic rate constants associated with the linear and non‐linear homopolymerizations of styrene, vinyl acetate, methyl methacrylate and butyl acrylate, overall molecular weight distributions and averages of the MWD were calculated using the NFT. Good agreement with the expected behavior, with MMA and STY not gelling while BA and VAc do, was obtained. It is concluded that the NFT produces coherent and reliable performance for known polymerization systems, whether linear or non‐linear.magnified image
A localized mixed‐hybrid method for imposing interfacial constraints in the extended finite element method (XFEM)
Authors: Zilian A. , Fries T.‐P. 
Published: March 2009

AbstractThe paper proposes an approach for the imposition of constraints along moving or fixed immersed interfaces in the context of the extended finite element method. An enriched approximation space enables consistent representation of strong and weak discontinuities in the solution fields along arbitrarily‐shaped material interfaces using an unfitted background mesh. The use of Lagrange multipliers or penalty methods is circumvented by a localized mixed hybrid formulation of the model equations. In a defined region in the vicinity of the interface, the original problem is re‐stated in its auxiliary formulation. The availability of the auxiliary variable enables the consideration of a variety of interface constraints in the weak form. The contribution discusses the weak imposition of Dirichlet‐ and Neumann‐type interface conditions as well as continuity requirements not fulfilled a priori by the enriched approximation. The properties of the proposed approach applied to two‐dimensional linear scalar‐ and vector‐valued elliptic problems are investigated by studying the convergence behavior. Copyright © 2009 John Wiley & Sons, Ltd.
The histone deacetylase inhibitor LBH589 inhibits expression of mitotic genes causing G2/M arrest and cell death in head and neck squamous cell carcinoma cell lines
Authors: Prystowsky Michael B , Adomako Alfred , Smith Richard V , Kawachi Nicole , McKimpson Wendy , Atadja Peter , Chen Quan , Schlecht Nicolas F , Parish Joanna L , Childs Geoffrey , Belbin Thomas J 
Published: March 2009

AbstractHead and neck squamous cell carcinoma represents a complex set of neoplasms arising in diverse anatomical locations. The site and stage of the cancer determine whether patients will be treated with single or multi‐modality therapy. The HDAC inhibitor LBH589 is effective in treating some haematological neoplasms and shows promise for certain epithelial neoplasms. As with other human cancer cell lines, LBH589 causes up‐regulation of p21, G2/M cell cycle arrest, and cell death of human HNSCC cell lines, as measured using flow cytometry and cDNA microarrays. Global RNA expression studies following treatment of the HNSCC cell line FaDu with LBH589 reveal down‐regulation of genes required for chromosome congression and segregation (SMC2L1), sister chromatid cohesion (DDX11), and kinetochore structure (CENP‐A, CENP‐F, and CENP‐M); these LBH589‐induced changes in gene expression coupled with the down‐regulation of MYC and BIRC5 (survivin) provide a plausible explanation for the early mitotic arrest and cell death observed. When LBH589‐induced changes in gene expression were compared with gene expression profiles of 41 primary HNSCC samples, many of the genes that were down‐regulated by LBH589 showed increased expression in primary HNSCC, suggesting that some patients with HNSCC may respond to treatment with LBH589. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
COX‐2 is induced by HGF stimulation in Met‐positive thyroid papillary carcinoma cells and is involved in tumour invasiveness
Authors: Scarpino Stefania , Duranti Enrico , Stoppacciaro Antonella , Pilozzi Emanuela , Natoli Guido , Sciacchitano Salvatore , Luciani Emidio , Ruco Luigi 
Published: March 2009

AbstractThyroid papillary carcinoma (TPC) cells express high levels of cytoplasmic cyclo‐oxygenase 2 protein. Analysis of microdissected samples of the tumour and of the paired normal thyroid tissue confirmed that mRNA transcripts for cyclo‐oxygenase 2 (COX‐2) were significantly more numerous in the tumour (7.6 ± 13‐fold; p = 0.01). High levels of COX‐2 mRNA were not associated with age, sex, tumour size or lymph node metastasis. COX‐2 was not homogeneously expressed throughout the tumour, but was significantly higher at the tumour invasion front. Hepatocyte growth factor (HGF) can up‐regulate the expression of COX‐2 mRNA. A marked increase in COX‐2 mRNA levels was observed in 8/8 primary TPC cultures after HGF stimulation (6.3 ± 6‐fold) and in two papillary carcinoma cell lines (TPC‐1 and NPA). Specific involvement of the high‐affinity HGF receptor (Met protein) was suggested by the observation that PHA‐665752, an inhibitor of the catalytic activity of c‐Met kinase, caused a 54% reduction of the hepatocyte growth factor‐induced COX‐2 up‐regulation. The possibility that HGF–Met interactions also had a causative role in the up‐regulation of COX‐2 in vivo was investigated in 30 tumour samples, where it was found that there was a statistically significant correlation (p = 0.001, r = 0.85) in the levels of expression of MET and COX‐2 RNAs. The biological role of COX‐2 in TPC cells was investigated by treating the TPC cell lines with the specific COX‐2 inhibitor NS‐398. It was found that NS‐398 treatment significantly reduced the migration (50–75%) and invasiveness (47–92%) of tumour cells, but did not alter cell proliferation. Our data suggest that the increased expression of Met protein in TPC cells has a role in up‐regulating the expression of COX‐2, which in turn contributes to the invasive capacity of TPC cells. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.