bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strontium‐isotope stratigraphy as a constraint on the age of condensed levels: examples from the Jurassic of the Subbetic Zone (southern Spain)
Authors: NIETO LUIS M. , RUIZ‐ORTIZ PEDRO A. , REY JAVIER , BENITO Mª ISABEL 
Published: October 2007

AbstractCondensed levels are often characterized by reworked fossils that may lead to incorrect age assessments. Strontium‐isotope stratigraphy is an important chronostratigraphic tool that can be used to verify the biostratigraphic information from condensed beds. This paper describes a study of the 87Sr/86Sr isotope ratios of 56 belemnite samples collected from 28 stratigraphic sections of the boundary between the Upper Member of the Gavilán Formation and the Zegrí Formation (Pliensbachian, Subbetic Zone). The petrographic and geochemical data (δ18O, δ13C, concentrations of Fe, Mn and Mg, and the Sr/Mn ratio) suggest that the belemnites have preserved their original marine geochemical composition. After plotting the samples in diagrams of 87Sr/86Sr values against time according to their biostratigraphic age, four different groups (A, B, C and D) were obtained with respect to the reference curve. In groups A and B, the age deduced from the Sr‐isotope ratio is in total or partial agreement, respectively, with the biostratigraphic age; therefore the 87Sr/86Sr ratio is a good method for the dating, correlation and assessment of biostratigraphic results. In groups C and D, the SIS age and the biostratigraphic age do not coincide. A graphic procedure is presented as a suitable methodology to constrain the age of the samples showing an SIS age that differs from the relative age deduced (by biostratigraphy or stratigraphic correlation) for the bed they were collected in. These situations are interpreted as being the result of reworking of the belemnites (group C) or ammonites (group D) that are included in condensed levels. These condensed levels formed during the maximum flooding event that led to the drowning of the Gavilán carbonate platform. The methodology supplied in this paper represents a valuable tool in identifying reworking processes, improving correlation and constraining biochronostratigraphic results. The values of 87Sr/86Sr represent a new contribution to the data set of 87Sr/86Sr ratios for the Pliensbachian.
Hydroa vacciniforme‐like Epstein‐Barr virus‐associated monoclonal T‐lymphoproliferative disorder in a child
Authors: Wu Yu‐Hung , Chen Hsiu‐Chin , Hsiao Pa‐Fan , Tu Mei‐Ing , Lin Yang‐Chih , Wang Tao‐Yuan 
Published: October 2007

Hydroa vacciniforme (HV) is a chronic photosensitivity disorder induced by ultraviolet radiation. Hydroa vacciniforme‐like lymphoma is a rare cutaneous T‐cell lymphoma occurring mainly in childhood. Recent studies have demonstrated an association between chronic latent Epstein‐Barr virus (EBV) infection and both the benign skin disorder and the lymphoma. The authors report a 6‐year‐old boy with chronic EBV infection, HV‐like skin eruptions, and chronic hepatitis. Histopathologic examination of a skin biopsy specimen demonstrated epidermal ballooning degeneration and dense superficial and deep perivascular and periappendageal lymphoid cell infiltrates extending to the fat lobules. Some blood vessels in the deep plexus were infiltrated by predominantly CD4+ and TIA‐1+ cytotoxic T cells. The EBV genomes were found within tissue from three skin biopsies and peripheral blood cells. Monoclonal T‐cell receptor gene rearrangement was present in skin biopsy specimens. Although no lymphoma has been found during 2 years of follow‐up treatment, the possibility of lymphoma developing out of the current smoldering stage is of concern. The clinical manifestations of lymphoproliferative disorder and chronic active EBV infection are discussed.
Lipoid proteinosis: a case series from Istanbul
Authors: Baykal Can , Topkarci Zeynep , Yazganoglu K. Didem , Azizlerli Gülsevim , Baykan Betül 
Published: October 2007

AbstractBackground  Lipoid proteinosis (LP) is a very rare genodermatosis. The literature on LP consists of case reports only. As we have observed 14 LP patients belonging to nine different families in the last 15 years in our practice, we decided to review all reported Turkish LP patients in this 15‐year period, and noted 37 diagnosed cases. The reasons for this relatively large number of cases, the clinical features of the patients, and the associations of LP with other clinical conditions are described in this article.Methods  Fourteen LP patients followed in our university clinic in Istanbul were scrutinized with regard to their demographic and clinical features. Diagnoses were established using clinical features, with histopathologic confirmation in 13 cases.Results  All but one of the patients had a history of consanguinity, or at least a marriage of parents from the same village. Typical cutaneous signs of LP and hoarseness of the voice were observed in all patients. Two patients of the same pedigree had insulin‐dependent diabetes mellitus (IDDM), two patients from two different pedigrees had short stature, one patient had multinodular toxic goiter, and one patient had celiac disease.Conclusion  LP is not rare in Turkey as consanguineous marriage is still a social problem, especially in some rural areas. The disease is not limited to a particular geographic region in Turkey. Short stature was observed in two cases from two different families, an association not reported previously; the association of LP with IDDM in one pedigree was thought to be coincidental.
Clinical characteristics of generalized idiopathic pruritus in patients from a tertiary referral center in Singapore
Authors: T.‐J. Goon Anthony , Yosipovitch Gil , Chan Yiong‐Huak , Goh Chee‐Leok 
Published: October 2007

AbstractBackground  Generalized idiopathic pruritus is a rarely studied heterogeneous condition, where patients with non‐inflamed, non‐pathologic skin complain of widespread pruritus.Methods  We had administered a detailed pruritus questionnaire, based on the short form of the McGill pain questionnaire, on 75 patients with this condition.Results  All 75 patients recruited with generalized idiopathic pruritus completed the questionnaire. In 92% of the patients, pruritus appeared on a daily basis. Most patients experienced pruritus at night (56%) and in the evening (61%) and 69% had difficulty falling asleep. Pruritus mainly involved the legs (79%), arms (76%) and back (68%). Accompanying symptoms were heat sensation (17%) and pain (13%). 80% had been prescribed antihistamines, of whom 56% obtained short‐term relief. The sensation of itch has been reported to be crawling (33%), tickling (20%), stinging (19%) and burning (19%). Patients also reported that the itch was unbearable (73%), bothersome (72%), annoying (67%) and worrisome (45%). The worst‐state VAS scores were significantly higher in patients who reported agitation (P = 0.006), difficulty in concentration (P= 0.010) and anxiety (P= 0.033).Conclusion  This study describes the sensory and affective dimensions of generalized idiopathic pruritus, and this questionnaire has been found to be a useful tool for evaluating pruritus in this condition.
Aplasia cutis congenita: a rare cutaneous sign of split cord malformations
Authors: Izci Yusuf , Gönül Müzeyyen , Secer Halil Ibrahim , Gönül Engin 
Published: October 2007

AbstractBackground  Aplasia cutis congenita (ACC) is characterized by the absence of a portion of skin in a localized or widespread area at birth. It manifests usually as a solitary defect on the scalp, but sometimes may occur on the face, trunk, or limbs. ACC is most often a benign isolated defect, but can be associated with other physical anomalies or malformation syndromes. A few cases have been reported in which patients with split cord malformation (SCM) have presented with ACC.Methods  Two patients with SCM are reported.Results  Both patients presented with ACC and abnormal hair growth on their backs. Type II SCM was detected in the first patient and Type I SCM in the second. No surgical treatment was performed because the patients were neurologically intact.Conclusions  ACC may seldom manifest as a skin marker of SCM. Our patients are unique examples of SCM presenting with ACC, but not requiring surgery. It is important to recognize ACC as a cutaneous sign of SCM, and to refer these patients to radiologic evaluation as soon as possible.
Screening for common dermatologic disorders amongst Israeli adolescents
Authors: Wohl Yonit , Freidman T. , Brenner S. , Bar Dayan Y. 
Published: October 2007

AbstractBackground  Dermatologic disorders amongst adolescents tend to overlap with general adult dermatology, but specific data are scarce. This study was undertaken to assess the prevalence of common skin disorders in 17‐year‐old Israeli military conscripts.Methods  Military recruits who underwent medical examination over approximately 1 year were included. Dermatology specialists evaluated and classified those with suspected skin disorders into categories of suitability for military tasks. Data were computerized for analysis of prevalences. Risk ratios for each category were determined for men and women.Results  Of the 94,806 adolescents, 36,511 (38.5%) women and 58,295 (61.5%) men, the most prevalent diagnoses were hyperhidrosis, multiple nevi, atopic dermatitis, keratinization disorders (mostly psoriasis), and contact dermatitis. The most prominent gender differences were in hyperhidrosis, contact dermatitis, and collagen diseases.Conclusion  Major dermatologic problems in adolescents are documented, and the findings may be useful for the military, employers, and general health services.
DPT vaccine‐induced lipoatrophy: an observational study
Authors: Sardana Kabir , Garg Vijay K. , Bhushan Premanshu , Relhan Vineet , Sharma Suvasini 
Published: October 2007

AbstractIntroduction  Diphtheria Pertussis Tetanus (DPT) vaccine is universally used in infants and children. It is generally safe and well tolerated. Local reactions such as erythema, induration, palpable nodules, and injection site abscess are well known. Injection site lipoatrophy has not been reported earlier.Patients and Methods  Retrospective review of all cases presenting with lipoatrophy developing at injection site following DPT administration between 2000–2005 in 3 hospitals in New Delhi, India was performed. In each case, the patients were extensively evaluated for other possible causes of lipoatrophy.Results  8 infants (2 boys & 6 girls), age range 4–12 months, had presented with injection site lipoatrophy following DPT vaccination. The duration between the last injection and lipoatrophy ranged from 4 to 8 weeks. All had been administered the vaccine in the buttock instead of the thigh, as generally recommended in infants. Majority (6/8) developed lipoatrophy after the second dose. No systemic causes were found.Conclusion  DPT vaccine may, in rare instances, lead to injection site lipoatrophy. Inadvertent administration into the subcutaneous fat of the buttock may have been causative. Other possible mechanisms are discussed. Paramedics and general practitioners need to be educated to administer intramuscular vaccines in the thigh in infants and young children.
Imported tungiasis: a report of 19 cases and review of the literature
Authors: Veraldi Stefano , Valsecchi Marta 
Published: October 2007

AbstractTungiasis is an infestation caused by penetration in the skin of the gravid female of the flea Tunga penetrans. In the period 1991–2006, 19 patients with imported tungiasis were observed at our Institute. All patients were subjected to general and dermatological examination, laboratory tests (including bacteriological examinations) and surgical excision of the lesions with histopathological examination. In all patients tetanus prophylaxis was made. All patients were followed up for at least six weeks. Thirteen patients were males (68.4%) and 6 females (31.6%). The age ranged from 3 to 71 years (average age: 37.8 years). Eleven patients (57.9%) contracted the infestation in Central and South America and 8 (42.1%) in Africa. In 17 patients (89.5%) tungiasis was localized to the feet, in one to the hands and in one to a thigh. In 13 patients (68.4%) the infestation was characterized by a single lesion; in the other 6 patients (31.6%) the lesions were two. In 15 patients (78.9%) tungiasis was characterized by a papular or nodular lesion. Crusted (2 patients), pustular (2 patients) and bullous (1 patient) lesions were also observed; furthermore, one patient presented with a plantar wart‐like lesion. In only one patient bacteriological examinations showed the growth of Staphylococcus aureus and Escherichia coli. All patients healed without complications.
Development of hepatic granulomas in patients receiving pegylated interferon therapy for recurrent hepatitis C virus post liver transplantation
Authors: Fiel M.I. , Shukla D. , Saraf N. , Xu R. , Schiano T.D. 
Published: October 2007

Abstract: Infrequently, hepatitis C (HCV) appears to be the cause of hepatic granulomas. Interferon therapy for HCV has been increasingly associated with the development of sarcoidosis.Aims. We sought to determine the incidence of hepatic granulomas in patients with recurrent HCV post liver transplantation (LT).Methods. Between 1994 and 2005, 820 patients were transplanted for HCV at our institution. The pathology database was searched for patients having recurrent HCV and granulomas. At Mount Sinai Medical Center, protocol biopsies have been performed for the last 2 years in patients receiving pegylated interferon‐α2b and ribavirin (PEG) for recurrent HCV. Review of slides from explanted livers, pre‐ and post‐perfusion biopsies, and all allograft biopsies were evaluated. Lipogranulomas were excluded because of their frequent association with steatosis.Results. A total of 10,225 liver biopsies were performed on HCV patients, and 25 (0.24%) showed non‐caseating epithelioid granulomas. Hepatic granulomas were detected in 14 post‐LT HCV patients; 9 patients received PEG. Typically, only 1 lobular granuloma was found. None of these patients had granulomas in the native liver or in any biopsy before interferon therapy; 6/9 patients had undetectable HCV‐RNA levels, and 4 had sustained viral response. No other cause for granuloma formation was identified in the 6 patients.Conclusions. Hepatic granulomas are infrequently found in HCV liver biopsies and rarely found in post‐LT biopsies with recurrent HCV. When present, they occur more commonly in patients receiving and virologically responding to PEG therapy. The presence of granulomas in patients with HCV being treated with PEG may not warrant an extensive etiologic work‐up for granulomatous hepatitis unless otherwise clinically indicated.
Preservation of Phakopsora pachyrhizi Uredospores
Authors: Furtado G. Q. , Alves S. A. M. , Czermainski A. B. C. , Massola N. S. 
Published: October 2007

AbstractThis study compared different temperatures and dormancy‐reversion procedures for preservation of Phakopsora pachyrhizi uredospores. The storage temperatures tested were room temperature, 5°C, −20°C and −80°C. Dehydrated and non‐dehydrated uredospores were used, and evaluations for germination (%) and infectivity (no. of lesions/cm2) were made with fresh harvested spores and after 15, 29, 76, 154 and 231 days of storage. The dormancy‐reversion procedures evaluated were thermal shock (40°C/5 min) followed or not by hydration (moist chamber/24 h). Uredospores stored at room temperature were viable only up to a month of storage, regardless of their hydration condition. Survival of uredospores increased with storage at lower temperatures. Dehydration of uredospores prior to storage increased their viability, mainly for uredospores stored at 5°C, −20°C and −80°C. At 5°C and −20°C, dehydrated uredospores showed increases in viability of at least 47 and 127 days, respectively, compared to non‐dehydrated spores. Uredospore germination and infectivity after storage for 231 days (7.7 months), could only be observed at −80°C, for both hydration conditions. At this storage temperature, dehydrated and non‐dehydrated uredospores exhibited 56 and 28% of germination at the end of the experiment, respectively. Storage at −80°C also maintained uredospore infectivity, based upon levels of infection frequency, for both hydration conditions. Among the dormancy‐reversion treatments applied to spores stored at −80°C, those involving hydration allowed recoveries of 85 to 92% of the initial germination.
Horse mackerel egg identification using DNA methodology
Authors: Karaiskou Nikoletta , Triantafyllidis Alexander , Alvarez Paula , Lopes Placida , Garcia‐Vazquez Eva , Triantaphyllidis Costas 
Published: October 2007

AbstractEgg identification in plankton samples is usually needed for purposes of stock assessment. Until recently, only morphological characters were used for identifying the eggs of different fish species. Late developmental stages are easily distinguishable due to pigmentation as well as egg and oil globule size range. However, for early stages, these characteristics are difficult to be discriminated and may overlap with other species. European horse mackerel species (Trachurus trachurus, T. mediterraneus, T. picturatus) overlap significantly in their spawning areas in some European waters. Because of the fact that their eggs are morphologically similar, genetic methodologies are needed to identify eggs and larvae to species correctly. In the present study, formalin‐ and ethanol‐preserved eggs were tested to estimate the efficacy of genetic methodologies for species identification of eggs when different preservatives are used. A 370‐bp fragment of cytochrome b was successfully amplified followed by restriction fragment analysis with two restriction enzymes aiming to identify the eggs to species. Horse mackerel egg identification was accomplished with the maximum success in ethanol‐preserved eggs. However, it seems that various preservatives had different effects on the DNA quality of eggs as genetic identification was less successful in formalin‐preserved eggs. Preserving in ethanol a part of the eggs obtained in plankton surveys is suggested for purposes of accurate genetic identification, even if their morphological features are distorted in time.
Association between AFLP‐based genetic distance and hybrid performance in tropical maize
Authors: Kiula B. A. , Lyimo N. G. , Botha A.‐M. 
Published: October 2007

AbstractIdentifying the best inbred combinations for the development of commercial hybrid maize varieties remains the main challenge to maize breeders. The aim of this work was to study associations between the genetic distance (GD) of 21 inbreds and the corresponding F1 phenotypic data. Furthermore, the impact of grouping lines into genetically similar clusters was investigated. The 21 inbred lines were fingerprinted using amplified fragment length polymorphism markers. Parents and 210 F1 progeny were evaluated in the field. Joint data analysis mostly revealed a tighter association between GD and the F1 performance or mid parent heterosis in the intergroup than in the intragroup crosses. Despite these correlations, intergoup crosses should always be field‐tested before their release. Crosses showing low GD values should be discarded to avoid field‐testing costs. Better F1 hybrid performance predictions can be achieved by integrating molecular and F1 phenotypic data.
Beschreibung eines Verfahrens zum unbhtigen Transfer boviner Embryonen
Authors: Von Lampeter W. W. , Kruff B. , Rieger J. , Wagner H. G. , Ertel J. 
Published: October 2007

Inhalt: Der beschriebene Embryotransferset ermöglicht eine optimale Plazierung des Embryos in der Spitze des Uterushorns. Eine einmalige Injektion von 500 mg Proge‐steron in Üliger Lösung, ca. 2 Stunden vor dem Transfer, verbessert die Trächtigkeits‐rate. Die erzielte Trächtigkeitsrate beträgt 59 % (31 Trächtigkeiten aus 50 Transfers). Der Transfer kann von Besamungstechnikern nach kurrer Einarbeitungszeit durch‐geführt werden.
Einfluß wiederholter Hodenbiopsien auf die postnatale Entwicklung des Rinderhodens
Authors: Fischerleitner Von F. , Sinowatz F. , Möstl E. 
Published: October 2007

Inhalt Von 44 Mutterschafen mit heterozygoter, autosomaler, interchromosomaler, balancierter Translokation 2n=54 XX t (1p‐;20q+) wurden während 147 Brunstzyklen innerhalb von 3 Jahren 2mal wöchentlich Blutproben entnommen. Gedeckte Muttertiere mit ansteigendem α‐Fetoproteingehalt (AFP) im Serum sind als tragend anzusehen. Es wurden 58% sterile Zyklen und 41,71% fertile Zyklen beobachtet, während nur 17,4% Lämmer geboren wurden. Das Auftreten von fertilen Zyklen, die Resorption von Zygoten mit unbalanciertem Karyotyp und die verlängerten Zyklen der heterozygoten Mutterschafe mit Chromosomentranslokation wird diskutiert.Contents: Fetoprotein evaluation in ewes with heterozygous translocation During 147 heat cycles within 3 years we took venous blood from 44 ewes with heterozygous, autosomal, interchromosomal balanced translocation 2n = 54 XX t (1p‐;20q+). Mated ewes with an increase of α‐Fetoprotein in the serum are pregnant. We observed 58% sterile heat cycles and 41,7% fertile heat cycles; whereas only 17,4% lambs were born. The appearance of fertile cycles, the resorption of cygotes with unbalanced karyotypes and the prolonged cycles of heterorygous ewes with chromosome translocation are in discussion.Wir danken der DFG für die finanzielle Unterstutzung dieser Arbeit.
Überprüfung der Anwendbarkeit eines Mikrotiter‐Enzymimmuntests für Nachgemelk zur Fruchtbarkeitsanalyse bei der Kuh
Authors: Claus Von R. , Münster E. , Dehnhard M. 
Published: October 2007

InhaltEin kommerziell verfügbarer Milchprogesterontest auf Mikrotiterplatten wurde auf seine Zuverlässigkeitskriterien für die Anwendung zur Fruchtbarkeitsanalyse bei der Milchkuh überprüft und Grenzwerte für Messungen im Nachgemelk durch Vergleich mit dem Milchfett‐RIA erstellt. Im Mikrotitertest liefern Nachgemelksproben, die auch tiefgefroren werden können, zuverlässige Meβwerte für die Probenahmetage 0,6 und 21. Als Empfindlichkeitsgrenze wurden 2 pg pro Messung ermittelt, die Variationskoeffizienten liegen für die Inter‐Assay‐Varianz zwischen 11 und 14%, für die IntraAssay‐Varianz zwischen 12 und 17% und für die Genauigkeit zwischen 12 und 17%. Der Vergleich des Mikrotitertests, der in weniger als 3 Stunden durchführbar ist, mit dem radioimmunologischen Verfahren ergab für Proben vom Tag 0, Tag 6 und Tag 21 folgende Grenzwerte (Sollwerte): Tag 0 < 2 ng Progesteron/ml Milch, Tag 6 > 2 ng Progesteron/lml Milch, Tag 21 >10 ng Progesteron/ml Milch. Das Meβuerfahren kommt somit für einen Praxiseinsatz in derentralen Labors sehr wohl in Frage, kann jedoch für spezietle Fragestellungen Präzisionsuerfahren in Zentrallabors nicht ersetzen.Contents: Evaluation of the reliability criteria of a Microtitre‐Enzymeimmunoassay in strippings for fertility screening in the dairy cowThe reliability criteria of a commercially available Milkprogesterone assay using microtitre plates were established and the applicability (including discriminatory levels) for fertility screening in strippings of the dairy cow was investigated. The sensitivity was found to be 2 pg/well and the following coefficients of variation were calculated: Interassay‐variation (11–14%), intra‐assay‐variation (12–17%) and accuracy (12–17%). The assay is performed in less than three hours and provides reliable values to differentiate between samples from days 0 (AI), 6 and 21. The discriminatory levels are < 2 ng/ml milk (day 0), > 2 ng/ml (day 6) and > 10 ng/ml (day 21).