bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of an anesthesia residency teaching service on anesthesia-controlled time and postsurgical patient outcomes: a retrospective observational study on 15,084 surgical cases
Authors: Lynch Davene , Mongan Paul D. , Hoefnagel Amie L. 
Published: April 2024

Abstract
Background
Limited data exists regarding the impact of anesthesia residents on operating room efficiency and patient safety outcomes. This investigation hypothesized that supervised anesthesiology residents do not increase anesthesia-controlled or prolonged extubation times compared to supervised certified registered nurse anesthetists (CRNA)/certified anesthesiologist assistants (CAA) or anesthesiologists working independently. Secondary objectives included differences in critical outcomes such as intraoperative hypotension, cardiac and pulmonary complications, acute kidney injury, and mortality.

Methods
This retrospective single-center 24-month (January 1, 2020- December 31, 2021) cohort focused on primary outcomes of anesthesia-controlled times and prolonged extubation (>15 min) with additional assessment of secondary patient outcomes in adult patients having general anesthesia with an endotracheal tube or laryngeal mask airway for elective non-cardiac surgery. The study excluded sedation, obstetric, endoscopic, ophthalmology, and non-operating room procedures. Procedures were divided into three groups: anesthesiologists working solo, anesthesiologists supervising residents, or anesthesiologists supervising CRNA/CAAs. After univariate analysis, multivariable models were constructed to control for the univariate cofactor differences in the primary and secondary outcomes.

Results
A total of 15,084 surgical cases met the inclusion criteria for this study for the three different care models: solo anesthesiologists (1,204 cases), anesthesiologist/resident pairing (3,146 cases), and anesthesiologist/CRNA/CAA (14,040 cases). Before multivariate analysis, the resident group exhibited longer anesthesia-controlled times (median, [interquartile range], 26.1 [21.7–32.0], p < 0.001), compared to CRNA/CAA (23.9 [19.7–29.5]), and attending-only surgical cases (21.0 [17.9–25.4]). After adjusting for covariates in a general linear regression model (age, BMI, ASA classification, comorbidities, arterial line insertion, surgical service, and surgical location), there were no significant differences in the anesthesia-controlled times between the provider groups. Prolonged extubation times (>15 min) were significantly less common in the anesthesiologist-only group compared to the other groups (p < 0.001). Despite these time differences, there were no clinically significant differences among the groups in postoperative pulmonary or cardiac complications, renal impairment, or the 30-day mortality rate of patients.

Conclusion
Anesthesia residents do not increase anesthesia-controlled operating room times or adversely affect clinically relevant patient outcomes compared to anesthesiologists working independently or supervising certified registered nurse anesthetists or certified anesthesiologist assistants.
Liquid biopsy techniques and lung cancer: diagnosis, monitoring and evaluation
Authors: Ren Fei , Fei Qian , Qiu Kun , Zhang Yuanjie , Zhang Heyang , Sun Lei 
Published: April 2024

AbstractLung cancer stands as the most prevalent form of cancer globally, posing a significant threat to human well-being. Due to the lack of effective and accurate early diagnostic methods, many patients are diagnosed with advanced lung cancer. Although surgical resection is still a potential means of eradicating lung cancer, patients with advanced lung cancer usually miss the best chance for surgical treatment, and even after surgical resection patients may still experience tumor recurrence. Additionally, chemotherapy, the mainstay of treatment for patients with advanced lung cancer, has the potential to be chemo-resistant, resulting in poor clinical outcomes. The emergence of liquid biopsies has garnered considerable attention owing to their noninvasive nature and the ability for continuous sampling. Technological advancements have propelled circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), extracellular vesicles (EVs), tumor metabolites, tumor-educated platelets (TEPs), and tumor-associated antigens (TAA) to the forefront as key liquid biopsy biomarkers, demonstrating intriguing and encouraging results for early diagnosis and prognostic evaluation of lung cancer. This review provides an overview of molecular biomarkers and assays utilized in liquid biopsies for lung cancer, encompassing CTCs, ctDNA, non-coding RNA (ncRNA), EVs, tumor metabolites, TAAs and TEPs. Furthermore, we expound on the practical applications of liquid biopsies, including early diagnosis, treatment response monitoring, prognostic evaluation, and recurrence monitoring in the context of lung cancer.
Nucleosome reorganisation in breast cancer tissues
Authors: Jacob Divya R. , Guiblet Wilfried M. , Mamayusupova Hulkar , Shtumpf Mariya , Ciuta Isabella , Ruje Luminita , Gretton Svetlana , Bikova Milena , Correa Clark , Dellow Emily , Agrawal Shivam P. , Shafiei Navid , Drobysevskaja Anastasija , Armstrong Chris M. , Lam Jonathan D. G. , Vainshtein Yevhen , Clarkson Christopher T. , Thorn Graeme J. , Sohn Kai , Pradeepa Madapura M. , Chandrasekharan Sankaran , Brooke Greg N. , Klenova Elena , Zhurkin Victor B. , Teif Vladimir B. 
Published: April 2024

AbstractBackgroundNucleosome repositioning in cancer is believed to cause many changes in genome organisation and gene expression. Understanding these changes is important to elucidate fundamental aspects of cancer. It is also important for medical diagnostics based on cell-free DNA (cfDNA), which originates from genomic DNA regions protected from digestion by nucleosomes.ResultsWe have generated high-resolution nucleosome maps in paired tumour and normal tissues from the same breast cancer patients using MNase-assisted histone H3 ChIP-seq and compared them with the corresponding cfDNA from blood plasma. This analysis has detected single-nucleosome repositioning at key regulatory regions in a patient-specific manner and common cancer-specific patterns across patients. The nucleosomes gained in tumour versus normal tissue were particularly informative of cancer pathways, with ~ 20-fold enrichment at CpG islands, a large fraction of which marked promoters of genes encoding DNA-binding proteins. The tumour tissues were characterised by a 5–10 bp decrease in the average distance between nucleosomes (nucleosome repeat length, NRL), which is qualitatively similar to the differences between pluripotent and differentiated cells. This effect was correlated with gene activity, differential DNA methylation and changes in local occupancy of linker histone variants H1.4 and H1X.ConclusionsOur study offers a novel resource of high-resolution nucleosome maps in breast cancer patients and reports for the first time the effect of systematic decrease of NRL in paired tumour versus normal breast tissues from the same patient. Our findings provide a new mechanistic understanding of nucleosome repositioning in tumour tissues that can be valuable for patient diagnostics, stratification and monitoring.
Saturnino Herrán’s portable murals: symbolism, material agency and conservation
Authors: Cano Nathael , de Lucio Oscar G. , Pérez Miguel , Mitrani Alejandro , Casanova Edgar , Ruvalcaba Sil José Luis 
Published: April 2024

Abstract
Alegoría de la construcción and Alegoría del trabajo were the first mural paintings commissioned to Saturnino Herrán (1887–1918), and they occupied a significant place in one of the most important schools in Mexico. The non-invasive methodology employed included different documentation phases and in situ multi-technique analyses. Hyperspectral imaging and digital radiography were used for a general characterization of the materials and were complemented with localized analyses by portable microscopy, and XRF and FORS spectroscopies. A complete identification of the original and the restoration palettes was achieved. Results from this work allowed us to understand the production context, painting technique, and conservation state, thus providing insights for the proper conservation of these murals, while also unveiling a new perception of the artist and his historical place among the muralist movement.
The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans
Authors: Rodríguez-Castro Laura , Durán Roberto E. , Méndez Valentina , Dorochesi Flavia , Zühlke Daniela , Riedel Katharina , Seeger Michael 
Published: April 2024

Abstract
Background
Bacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the protective role of FldX1 in P. xenovorans LB400 during the degradation of 4-hydroxyphenylacetate (4-HPA) and 3-hydroxyphenylacetate (3-HPA).

Methods
The functionality of FldX1 was evaluated in P. xenovorans p2-fldX1 that overexpresses FldX1. The effects of FldX1 on P. xenovorans were studied measuring growth on hydroxyphenylacetates, degradation of 4-HPA and 3-HPA, and ROS formation. The effects of hydroxyphenylacetates (HPAs) on the proteome (LC–MS/MS) and gene expression (qRT-PCR) were quantified. Bioaugmentation with strain p2-fldX1 of 4-HPA-polluted soil was assessed, measuring aromatic degradation (HPLC), 4-HPA-degrading bacteria, and plasmid stability.

Results
The exposure of P. xenovorans to 4-HPA increased the formation of ROS compared to 3-HPA or glucose. P. xenovorans p2-fldX1 showed an increased growth on 4-HPA and 3-HPA compared to the control strain WT-p2. Strain p2-fldX1 degraded faster 4-HPA and 3-HPA than strain WT-p2. Both WT-p2 and p2-fldX1 cells grown on 4-HPA displayed more changes in the proteome than cells grown on 3-HPA in comparison to glucose-grown cells. Several enzymes involved in ROS detoxification, including AhpC2, AhpF, AhpD3, KatA, Bcp, CpoF1, Prx1 and Prx2, were upregulated by hydroxyphenylacetates. Downregulation of organic hydroperoxide resistance (Ohr) and DpsA proteins was observed. A downregulation of the genes encoding scavenging enzymes (katE and sodB), and gstA and trxB was observed in p2-fldX1 cells, suggesting that FldX1 prevents the antioxidant response. More than 20 membrane proteins, including porins and transporters, showed changes in expression during the growth of both strains on hydroxyphenylacetates. An increased 4-HPA degradation by recombinant strain p2-fldX1 in soil microcosms was observed. In soil, the strain overexpressing the flavodoxin FldX1 showed a lower plasmid loss, compared to WT-p2 strain, suggesting that FldX1 contributes to bacterial fitness. Overall, these results suggest that recombinant strain p2-fldX1 is an attractive bacterium for its application in bioremediation processes of aromatic compounds.

Conclusions
The long-chain flavodoxin FldX1 improved the capability of P. xenovorans to degrade 4-HPA in liquid culture and soil microcosms by protecting cells against the degradation-associated oxidative stress.
Glutaminolysis regulates endometrial fibrosis in intrauterine adhesion via modulating mitochondrial function
Authors: Chen Pei , Ye Chaoshuang , Huang Yunke , Xu Bingning , Wu Tianyu , Dong Yuanhang , Jin Yang , Zhao Li , Hu Changchang , Mao Jingxia , Wu Ruijin 
Published: April 2024

Abstract
Background
Endometrial fibrosis, a significant characteristic of intrauterine adhesion (IUA), is caused by the excessive differentiation and activation of endometrial stromal cells (ESCs). Glutaminolysis is the metabolic process of glutamine (Gln), which has been implicated in multiple types of organ fibrosis. So far, little is known about whether glutaminolysis plays a role in endometrial fibrosis.

Methods
The activation model of ESCs was constructed by TGF-β1, followed by RNA-sequencing analysis. Changes in glutaminase1 (GLS1) expression at RNA and protein levels in activated ESCs were verified experimentally. Human IUA samples were collected to verify GLS1 expression in endometrial fibrosis. GLS1 inhibitor and glutamine deprivation were applied to ESCs models to investigate the biological functions and mechanisms of glutaminolysis in ESCs activation. The IUA mice model was established to explore the effect of glutaminolysis inhibition on endometrial fibrosis.

Results
We found that GLS1 expression was significantly increased in activated ESCs models and fibrotic endometrium. Glutaminolysis inhibition by GLS1 inhibitor bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES or glutamine deprivation treatment suppressed the expression of two fibrotic markers, α-SMA and collagen I, as well as the mitochondrial function and mTORC1 signaling in ESCs. Furthermore, inhibition of the mTORC1 signaling pathway by rapamycin suppressed ESCs activation. In IUA mice models, BPTES treatment significantly ameliorated endometrial fibrosis and improved pregnancy outcomes.

Conclusion
Glutaminolysis and glutaminolysis-associated mTOR signaling play a role in the activation of ESCs and the pathogenesis of endometrial fibrosis through regulating mitochondrial function. Glutaminolysis inhibition suppresses the activation of ESCs, which might be a novel therapeutic strategy for IUA.
Computer-aided analysis of radiological images for cancer diagnosis: performance analysis on benchmark datasets, challenges, and directions
Authors: Alyami Jaber 
Published: April 2024

AbstractRadiological image analysis using machine learning has been extensively applied to enhance biopsy diagnosis accuracy and assist radiologists with precise cures. With improvements in the medical industry and its technology, computer-aided diagnosis (CAD) systems have been essential in detecting early cancer signs in patients that could not be observed physically, exclusive of introducing errors. CAD is a detection system that combines artificially intelligent techniques with image processing applications thru computer vision. Several manual procedures are reported in state of the art for cancer diagnosis. Still, they are costly, time-consuming and diagnose cancer in late stages such as CT scans, radiography, and MRI scan. In this research, numerous state-of-the-art approaches on multi-organs detection using clinical practices are evaluated, such as cancer, neurological, psychiatric, cardiovascular and abdominal imaging. Additionally, numerous sound approaches are clustered together and their results are assessed and compared on benchmark datasets. Standard metrics such as accuracy, sensitivity, specificity and false-positive rate are employed to check the validity of the current models reported in the literature. Finally, existing issues are highlighted and possible directions for future work are also suggested.
Vegetation, fuels, and fire-behavior responses to linear fuel-break treatments in and around burned sagebrush steppe: are we breaking the grass-fire cycle?
Authors: Germino Matthew J. , Price Samuel “Jake” , Prichard Susan J. 
Published: April 2024

Abstract
Background
Linear fuel breaks are being implemented to moderate fire behavior and improve wildfire containment in semiarid landscapes such as the sagebrush steppe of North America, where extensive losses in perennial vegetation and ecosystem functioning are resulting from invasion by exotic annual grasses (EAGs) that foster large and recurrent wildfires. However, fuel-break construction can also pose EAG invasion risks, which must be weighed against the intended fire-moderation benefits of the treatments. We investigated how shrub reductions (mowing, cutting), pre-emergent EAG-herbicides, and/or drill seedings of fire-resistant perennial bunchgrasses (PBGs) recently applied to create a large fuel-break system affected native and exotic plant abundances and their associated fuel loading and predicted fire behavior.

Results
In heavily EAG-invaded areas, herbicides reduced EAG and total herbaceous cover without affecting PBGs for 2–3 years and reduced predicted fire behavior for 1 year (from the Fuel Characteristic Classification System). However, surviving post-herbicide EAG cover was still > 30%, which was sufficient fuel to exceed the conventional 1.2-m-flame length (FL) threshold for attempting wildfire suppression with hand tools. In less invaded shrubland, shrub reduction treatments largely reduced shrub cover and height by ~ half without increasing EAGs, but then redistributed the wood to ground level and increased total herbaceous cover. Herbicides and/or drill seeding after shrub reductions did not affect EAG cover, although drill seedings increased PBG cover and exotic forbs (e.g., Russian thistle). Fire behavior was predicted to be moderated in only one of the many yearly observations of the various shrub-reduction treatment combinations. Over all treatments and years, FLs were predicted to exceed 1.2 m in 13% of simulations under average (11 km h−1) or high (47 km h−1) wind speed conditions and exceed the 3.4-m threshold for uncontrollable fire in 11% of simulations under high-wind speeds only.

Conclusions
Predicted fire-moderation benefits over the first 4 years of fuel break implementation were modest and variable, but, generally, increases in EAGs and their associated fire risks were not observed. Nonetheless, ancillary evidence from shrublands would suggest that treatment-induced shifts from shrub to herbaceous fuel dominance are expected to improve conditions for active fire suppression in ways not readily represented in available fire models.
Perspectives of healthcare professionals on the use of immersive virtual reality in teenage and young adult oncology: a qualitative interview study
Authors: Deighan Mairi Therese , Simpson Daniel , Brigden Amberly , Cater Kirsten 
Published: April 2024

Abstract
Background
Applications of Virtual Reality (VR) in healthcare continue to grow at a rapid pace, yet the challenges and opportunities associated with VR from the perspective of clinicians and healthcare staff remain underexplored, particularly in the context of Teenage and Young Adult (TYA) oncology. This study aims to bridge this knowledge gap by interviewing clinicians and staff regarding the use of VR in TYA cancer care.

Method
Fourteen semi-structured qualitative interviews were conducted with a diverse range of healthcare professionals working in TYA oncology within the United Kingdom including oncologists, hematologists, clinical nurse specialists, clinical psychologists, radiographers, play specialists, clinical social workers and youth support coordinators. Data were analysed thematically with three themes and ten sub-themes developed.

Results
The findings reveal that TYA healthcare professionals are optimistic and enthusiastic about the potential of using VR to enhance cancer care for teenagers and young adults. However, they also expressed notable concerns related to the practical implementation of VR in clinical settings. These concerns included infection and safety risks, privacy and security concerns, cost implications, storage requirements and staff burden. Clinicians and staff proposed valuable applications for VR in TYA oncology specifically in patient distraction, physiotherapy, procedure preparation, and the delivery of psychotherapy, including acceptance and commitment therapy.

Conclusions
This research has demonstrated that despite an enthusiasm to adopt VR in TYA oncology there are still many challenges, both practical and ethical, that must be addressed to enable the successful implementation of VR in hospitals within the UK. Further research into applications of VR for TYA oncology is warranted, particularly in areas of psychology, physiotherapy, and procedure preparation.
Impact of Banking Fraud Awareness Booklet Among The Students of Satara
Authors: Samidha Samidha Walvekar 
Published: April 2024

With the growth of information and communication technology, the structure and nature of financial services delivery has also changed. Online banking or internet banking has emerged as a new and convenient way for using financial services like funds transfer, viewing account statement, bill payment, use of e-wallets etc. An upsurge in the use of devices connected with the internet and the convenience of online financial services has increased the risk of our hard-earned money being duped by cybercriminals.
The main aim of this study is to assess the impact of Banking fraud awareness Booklet regarding online among students of Satara.
The objectives of this study are to assess knowledge regarding awareness of online banking frauds among college going students, to determine the effectiveness of information booklet among college going students and also to find out association between socio-demographic variables and the booklet.
The results show the significant increase in mean scores as (mean pre 16.92 SD=2.591 to mean post 18.32 SD=1.842). The paired 't' test applied showed the calculated paired t value (5.291 at 0.05 level of significance). The study reveals that there is association between, class, department & course of the subjects and level of knowledge regarding awareness on online banking frauds.
The information booklet was effective in improving the knowledge of awareness regarding online banking frauds among the Undergraduate students.
Keywords: Online banking frauds, Banking fraud awareness booklet.
Revision total hip arthroplasty using a modular fluted, tapered revision femoral component and interlocking screws in Vancouver B3 periprosthetic fractures with insufficient bone at the isthmus
Authors: Fink Bernd , Ahmadian Arman , Sax Florian H. , Schuster Philipp 
Published: April 2024

AimsRevision total hip arthroplasty in patients with Vancouver type B3 fractures with Paprosky type IIIA, IIIB, and IV femoral defects are difficult to treat. One option for Paprovsky type IIIB and IV defects involves modular cementless, tapered, revision femoral components in conjunction with distal interlocking screws. The aim of this study was to analyze the rate of reoperations and complications and union of the fracture, subsidence of the stem, mortality, and the clinical outcomes in these patients.MethodsA total of 46 femoral components in patients with Vancouver B3 fractures (23 with Paprosky type IIIA, 19 with type IIIB, and four with type IV defects) in 46 patients were revised with a transfemoral approach using a modular, tapered, cementless revision Revitan curved femoral component with distal cone-in-cone fixation and prospectively followed for a mean of 48.8 months (SD 23.9; 24 to 112). The mean age of the patients was 80.4 years (66 to 100). Additional distal interlocking was also used in 23 fractures in which distal cone-in-cone fixation in the isthmus was < 3 cm.ResultsOne patient (2.2%) died during the first postoperative year. After six months, 43 patients (93.5%) had osseous, and three had fibrous consolidation of the fracture and the bony flap, 42 (91.3%) had bony ingrowth and four had stable fibrous fixation of the stem. No patient had radiolucency around the interlocking screws and no screw broke. One patient had non-progressive subsidence and two had a dislocation. The mean Harris Hip Score increased from of 57.8 points (SD 7.9) three months postoperatively to 76.1 points (SD 10.7) 24 months postoperatively.ConclusionThe 2° tapered, fluted revision femoral component with distal cone-in-cone-fixation, combined with additional distal interlocking in patients with bony deficiency at the isthmus, led to reproducibly good results in patients with a Vancouver B3 fracture and Paprosky type IIIA, IIIB, and IV defects with regard to union of the fracture, subsidence or loosening of the stem, and clinical outcomes.Cite this article: Bone Joint J 2024;106-B(4):344–351.