bluuebunny/crossref_metadata_2025_split huggingface dataset:
LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice
Authors: Volpatti Lisa R. , de Matos Salvador Norton , Borjas Gustavo , Reda Joseph , Watkins Elyse A. , Zhou Zhengjie , Nguyen Mindy , Solanki Ani , Fang Yun , Hubbell Jeffrey A. 
Published: March 2024

ABSTRACTAtherosclerosis is a chronic inflammatory disease associated with the accumulation of low-density lipoprotein (LDL) in arterial walls. Higher levels of the anti-inflammatory cytokine IL-10 in serum are correlated with reduced plaque burden. However, cytokine therapies have not translated well to the clinic, partially due to their rapid clearance and pleiotropic nature. Here, we engineered IL-10 to overcome these challenges by hitchhiking on LDL to atherosclerotic plaques. Specifically, we constructed fusion proteins in which one domain is IL-10 and the other is an antibody fragment (Fab) that binds to protein epitopes of LDL. In murine models of atherosclerosis, we show that systemically administered Fab-IL-10 constructs bind circulating LDL and traffic to atherosclerotic plaques. One such construct, 2D03-IL-10, significantly reduces aortic immune cell infiltration to levels comparable to healthy mice, whereas non-targeted IL-10 has no therapeutic effect. Mechanistically, we demonstrate that 2D03-IL-10 preferentially associates with foamy macrophages and reduces pro-inflammatory activation markers. This platform technology can be applied to a variety of therapeutics and shows promise as a potential targeted anti-inflammatory therapy in atherosclerosis.
Persistent Interruption in Parvalbumin Positive Inhibitory Interneurons: Biophysical and Mathematical Mechanisms
Authors: Upchurch Carol M , Knowlton Christopher J , Chamberland Simon , Canavier Carmen C 
Published: March 2024

AbstractPersistent activity in principal cells is a putative mechanism for maintaining memory traces during working memory. We recently demonstrated persistent interruption of firing in fast-spiking parvalbumin-expressing interneurons (PV-INs), a phenomenon which could serve as a substrate for persistent activity in principal cells through disinhibition lasting hundreds of milliseconds. Here, we find that hippocampal CA1 PV-INs exhibit type 2 excitability, like striatal and neocortical PV-INs. Modelling and mathematical analysis showed that the slowly inactivating potassium current Kv1 contributes to type 2 excitability, enables the multiple firing regimes observed experimentally in PV-INs, and provides a mechanism for robust persistent interruption of firing. Using a fast/slow separation of times scales approach with the Kv1 inactivation variable as a bifurcation parameter shows that the initial inhibitory stimulus stops repetitive firing by moving the membrane potential trajectory onto a co-existing stable fixed point corresponding to a non-spiking quiescent state. As Kv1 inactivation decays, the trajectory follows the branch of stable fixed points until it crosses a subcritical Hopf bifurcation then spirals out into repetitive firing. In a model describing entorhinal cortical PV-INs without Kv1, interruption of firing could be achieved by taking advantage of the bistability inherent in type 2 excitability based on a subcritical Hopf bifurcation, but the interruption was not robust to noise. Persistent interruption of firing is therefore broadly applicable to PV-INs in different brain regions but is only made robust to noise in the presence of a slow variable.Significance StatementPersistent activity in neuronal networks is thought to provide a substrate for multiple forms of memory. The architecture of neuronal networks across many brain regions involves a small number of inhibitory neurons that control many principal neurons. We propose that persistent silencing of fast-spiking parvalbumin-expressing inhibitory interneurons (PVINs) can result in persistent activity of principal neurons. We use a mathematical approach and computer simulations to investigate the mechanisms governing persistent interruption of firing in hippocampal and cortical PV-INs. We show how a slowly changing state of a particular ion channel controls the long-lasting silence imposed by persistent interruption. Overall, our results provide a conceptual framework that positions the persistent interruption of PV-INs firing as a potential mechanism for persistent activity in principal cells.
Nucleolar stress induced the formation of a nucleolar stress body via the NOSR-1/NUMR-1 axis inCaenorhabditis elegans
Authors: Hong Minjie , Zhou Xiaotian , Zeng Chenming , Xu Demin , Xu Ting , Liao Shimiao , Wang Ke , Zhu Chengming , Shan Ge , Huang Xinya , Chen Xiangyang , Feng Xuezhu , Guang Shouhong 
Published: March 2024

AbstractEnvironmental stimuli not only alter gene expression profiles but also induce structural changes in cells. How distinct nuclear bodies respond to cellular stress is poorly understood. Here, we identified a new subnuclear organelle named the nucleolar stress body (NoSB), the formation of which was induced by the inhibition of rRNA transcription or inactivation of rRNA processing and maturation inC. elegans. NoSB did not colocalize with other previously described subnuclear organelles. We conducted forward genetic screening and identified a new bZIP transcription factor, namednucleolarstress response-1 (NOSR-1), that is required for NoSB formation. The inhibition of rRNA transcription or inactivation of rRNA processing and maturation increasednosr-1expression. By using transcriptome analysis of wild-type animals subjected to different nucleolar stress conditions andnosr-1mutants, we identified that the SR-like protein NUMR-1 (nuclear localized metal responsive) is the target of NOSR-1. Interestingly, NUMR-1 is a component of NoSB and itself per se is required for the formation of NoSB. We concluded that the NOSR-1/NUMR-1 axis likely responds to nucleolar stress and mediates downstream stress-responsive transcription programs and subnuclear morphology alterations inC. elegans.
Matrix viscoelasticity controls epithelial cell mechanobiology through dimensionality
Authors: Ciccone Giuseppe , Oliva Mariana Azevedo Gonzalez , Versaevel Marie , Cantini Marco , Vassalli Massimo , Salmeron-Sanchez Manuel , Gabriele Sylvain 
Published: March 2024

AbstractIn recent years, matrix viscoelasticity has emerged as a potent regulator of fundamental cellular processes and has been implicated in promoting cancer progression. Alongside viscoelasticity, additional ECM cues have been shown to influence migration decision-making of cancer cells, and spatial confinement is now considered as a potential regulator of metastasis. However, our understanding of these complex processes predominantly relies on purely elastic hydrogels, and the exact relationship between matrix viscoelasticity and spatial confinement in driving epithelial cell mechanotransduction and migration during cancer progression remains unclear. Here, we systematically investigated the interplay between matrix stiffness, viscoelasticity and spatial confinement by engineering soft (∼0.3 kPa) and stiff (∼3 kPa) polyacrylamide hydrogels with varying degrees of viscous dissipation, mirroring the mechanical properties of healthy and tumoral conditions in breast tissue. We observed that viscoelasticity modulates cell spreading, focal adhesions and YAP nuclear import in opposite directions on soft and stiff substrates. Strikingly, viscoelasticity enhances migration speed and persistence on soft substrates, while impeding them on stiff substrates via actin retrograde flow regulation. Combining soft micropatterning with viscoelastic hydrogels, we also show that spatial confinement restricts cell migration on soft matrices regardless of matrix viscoelasticity and promotes migration on stiff matrices in a viscoelasticity-dependent fashion. Our findings establish substrate viscoelasticity as a key regulator of epithelial cell functions and unravel the role of the matrix dimensionality in this process.SignificanceWhile matrix elasticity has received significant attention, recent findings underscore the importance of its natural dissipative properties and spatial confinement in regulating cellular processes and tumour invasiveness. However, the intricate interplay between viscoelasticity and spatial confinement in orchestrating epithelial cell behaviour during cancer progression remains elusive. Using micropatterned viscoelastic hydrogels to replicate the mechanical properties encountered during breast tumour progression, we unveil that viscoelasticity modulates cell behaviour and mechanotransduction signals differently on soft and stiff substrates. Increased viscoelasticity enhances migration speed and persistence on soft substrates while impeding them on stiff substrates via actin retrograde flow regulation. Furthermore, spatial confinement restricts cell migration on soft matrices regardless of viscoelasticity, while promoting migration on stiff matrices in a viscoelasticity-dependent manner.
An ethologically relevant paradigm to assess visual contrast sensitivity in rodents
Authors: Calanni Juan S. , Aranda Marcos L. , Dieguez Hernán H. , Dorfman Damian , Schmidt Tiffany M. , Rosenstein Ruth E. 
Published: March 2024

ABSTRACTIn the animal kingdom, threat information is perceived mainly through vision. The subcortical visual pathway plays a critical role in the rapid processing of visual information-induced fear, and triggers a response. Looming-evoked behavior in rodents, mimicking response to aerial predators, allowed identify the neural circuitry underlying instinctive defensive behaviors; however, the influence of disk/background contrast on the looming-induced behavioral response has not been examined, either in rats or mice. We studied the influence of the dark disk/gray background contrast in the type of rat and mouse defensive behavior in the looming arena, and we showed that rat and mouse response as a function of disk/background contrast adjusted to a sigmoid-like relationship. Both sex and age biased the contrast-dependent response, which was dampened in rats submitted to retinal unilateral or bilateral ischemia. Moreover, using genetically manipulated mice, we showed that the three type of photoresponsive retinal cells (i.e., cones, rods, and intrinsically photoresponsive retinal ganglion cells (ipRGCs)), participate in the contrast-dependent response, following this hierarchy: cones ˃> rods ˃>>ipRGCs. The cone and rod involvement was confirmed using a mouse model of unilateral non-exudative age-related macular degeneration, which only damages canonical photoreceptors and significantly decreased the contrast sensitivity in the looming arena.
MORTALITY-ASSOCIATED SARS-COV-2 GENOMIC VARIANTS FROM PATIENTS HOSPITALIZED FOR SEVERE PNEUMONIA IN AGUASCALIENTES, MEXICO FROM 2020 TO 2023
Authors: Gomez Brian Muñoz , Gamboa Miriam Sarahi Lozano , Ceapa Corina Diana , Marmolejo Anastacio Palacios 
Published: March 2024

ABSTRACTBackgroundThe SARS-CoV-2 virus has caused a global health crisis, resulting in a significant loss of human lives. It is essential to report disease and mutation associations to provide ideas for public health interventions and preventive measures.MethodsIn this study, to determine the association between genomic variants and the severity of pneumonia caused by SARS-CoV-2, a sequencing analysis of 150 patient samples with confirmed COVID-19 was conducted. These samples were collected between 2021 and 2023 and isolated in Aguascalientes, Mexico.ResultsThe patient cohort had males and females ranging from 0 to 91 years old. Males accounted for 66% of the population analyzed. The Delta variant was the most prevalent lineage associated with deaths in 2021-2022, while the B.1.1.529 lineages emerged in mid-2022. Currently, the XBB lineage is the most commonly identified in Mexico. New mutations L95M and L46M in ORF 8 and ORF 9 were discovered in 30% and 20% of the sequences and are uniquely present in the studied population. These mutations are positively associated with patient death.ConclusionsThis study provides valuable data to aid in understanding the evolution of SARS-CoV-2 in specific populations and explores the severity of the disease and mutation correlations.
Temporal attention amplifies stimulus information in fronto-cingulate cortex at an intermediate processing stage
Authors: Zhu Jiating , Tian Karen J. , Carrasco Marisa , Denison Rachel N. 
Published: March 2024

AbstractThe human brain faces significant constraints in its ability to process every item in a sequence of stimuli. Voluntary temporal attention can selectively prioritize a task-relevant item over its temporal competitors to alleviate these constraints. However, it remains unclear when and where in the brain selective temporal attention modulates the visual representation of a prioritized item. Here, we manipulated temporal attention to successive stimuli in a two-target temporal cueing task, while controlling for temporal expectation with fully predictable stimulus timing. We used MEG and time-resolved decoding to track the spatiotemporal evolution of stimulus representations in human observers. We found that temporal attention enhanced the representation of the first target around 250 milliseconds after target onset, in a contiguous region spanning left frontal cortex and cingulate cortex. The results indicate that voluntary temporal attention recruits cortical regions beyond the ventral stream at an intermediate processing stage to amplify the representation of a target stimulus. This routing of stimulus information to anterior brain regions may provide protection from interference in visual cortex by a subsequent stimulus. Thus, voluntary temporal attention may have distinctive neural mechanisms to support specific demands of the sequential processing of stimuli.Significance statementWhen viewing a rapid sequence of visual input, the brain cannot fully process every item. Humans can attend to an item they know will be important to enhance its processing. However, how the brain selects one moment over others is little understood. We found that attending to visual information at a precise moment in time enhances visual representations around 250 ms after an item appears. Unexpectedly, this enhancement occurred not in the visual cortex, but in the left fronto-cingulate cortex. The involvement of frontal rather than posterior cortical regions in representing visual stimuli has not typically been observed for spatial or feature-based attention, suggesting that temporal attention may have specialized neural mechanisms to handle the distinctive demands of sequential processing.
Research on vibration characteristics of rocket engine flow pipeline
Authors: Yong Su , Wu-Qi Gong 
Published: March 2024

Purpose
Abnormal vibrations often occur in the liquid oxygen kerosene transmission pipelines of rocket engines, which seriously threaten their safety. Improper handling can result in failed rocket launches and significant economic losses. Therefore, this paper aims to examine vibrations in transmission pipelines.

Design/methodology/approach
In this study, a three-dimensional high-pressure pipeline model composed of corrugated pipes, multi-section bent pipes, and other auxiliary structures was established. The fluid–solid coupling method was used to analyse vibration characteristics of the pipeline under various external excitations. The simulation results were visualised using MATLAB, and their validity was verified via a thermal test.

Findings
In this study, the vibration mechanism of a complex high-pressure pipeline was examined via a visualisation method. The results showed that the low-frequency vibration of the pipe was caused by fluid self-excited pressure pulsation, whereas the vibration of the engine system caused a high-frequency vibration of the pipeline. The excitation of external pressure pulses did not significantly affect the vibrations of the pipelines. The visualisation results indicated that the severe vibration position of the pipeline thermal test is mainly concentrated between the inlet and outlet and between the two bellows.

Practical implications
The results of this study aid in understanding the causes of abnormal vibrations in rocket engine pipelines.

Originality/value
The causes of different vibration frequencies in the complex pipelines of rocket engines and the propagation characteristics of external vibration excitation were obtained.
Sustainability and accounting education in the Greek context
Authors: Nerantzidis Michail 
Published: March 2024

PurposeSustainable development (SD) is becoming an important area of research in accounting education. This paper examines if postgraduate accounting students in the Greek context present different awareness of SD issues and if this is related to their Master’s orientation. Moreover, it compares the results with other similar studies.Design/methodology/approachAn online questionnaire was designed based on Ebaid (2022) and Aleixo et al. (2021), and distributed to two different Master’s orientation programs. Then, descriptive statistics and contingency table analysis were performed to illustrate the main differences regarding the students’ responses.FindingsThe results indicate that the examined postgraduate students recognize the importance of SD for their future careers and require more knowledge about this issue. Moreover, it is presented that most of the postgraduate students adhere to the core sustainability principles (i.e. recycle, reuse and reduce), while only the students of environmental, social and governance (ESG) Master are significantly and actively engaged in social and environmental activities. Collectively, the results show that postgraduate students in Greece are more familiar with the term SD compared to participants in other similar studies in different contexts.Practical implicationsPlanners and decision-makers in higher education institutions could benefit from the findings to reconsider accounting education by introducing new courses and/or Master’s programs to promote knowledge and action for SD.Originality/valueThis is the first study, as far as we know, that examines the issue of sustainability and accounting education on postgraduate students.
What lies beneath: using student reflections to study the entrepreneurial mindset in entrepreneurship education
Authors: Larsen Inge Birkbak , Neergaard Helle 
Published: March 2024

PurposeThis research presents and evaluates a method for assessing the entrepreneurial mindset (EM) of students in higher education.Design/methodology/approachThe research considers EM a multi-variable psychological construct, which can be broken down into several conceptual sub-categories. Using data from a master course in entrepreneurship, the authors show how these categories can be applied to analyze students’ written reflections to identify linguistic markers of EM.FindingsThe research reports three main findings: analyzing student reflections is an appropriate method to explore the state and development of students’ EM; the theoretically-derived EM categories can be nuanced and extended with insight from contextualized empirical insights; and student reflections reveal counter-EM categories that represent challenges in the educator’s endeavor to foster students’ EM.Research limitations/implicationsThe commitment of resources to researching EM requires the dedication of efforts to develop methods for assessing the state and development of students’ EM. The framework can be applied to enhance the theoretical rigor and methodological transparency of studies of EM in entrepreneurship education.Practical implicationsThe framework can be of value to educators who currently struggle to assess if and how their educational design fosters EM attributes.Originality/valueThis inquiry contributes to the critical research discussion about how to operationalize EM in entrepreneurship education studies. The operationalization of a psychological concept such as EM is highly important because a research focus cannot be maintained on something that cannot be studied in a meaningful way.
A moderated-mediation model of training transfer from leadership intervention
Authors: Yaqub Yasmin , Dutta Tanusree , Dhir Swati 
Published: March 2024

PurposeGrounding on the goal-setting theory and flow theory, this study explored the mechanism underlying the association between transfer design (TD); identical elements and training transfer (TT). Specifically, the authors explored a moderated mediation process of trainer performance and motivation to improve work through learning (MTIWL) that has received less consideration in the TT literature.Design/methodology/approachData were collected using the retro-perspective survey method. The first survey was administered offline (t1: the day when leadership intervention was completed. Subsequently, trainees were requested to participate in an online survey (t2: 12–14 weeks later). In all, 355 executives participated.FindingsThe results of structural equation modeling (SEM) analyses suggested that trainees’ MTIWL mediational impact between leadership intervention triggers (transfer design and identical elements), and TT was supported. In addition, the indirect impact of these variables on TT was found to be significant when the trainer had high performance than when it was low. This confirmed the trainer’s performance as a potential moderator in the TT process.Practical implicationsThis study is limited to the exploration of leadership intervention variables on TT. The findings have implications for leadership professionals and scholars who use leadership intervention and motivation metrics to predict TT.Originality/valueThis study offers a moderated mediation mechanism for enhancing TT through leadership intervention triggers. The proposed conceptual model included MTIWL as mediator and trainer performance during leadership intervention as moderator.
The educational value of artificial intelligence in higher education: a 10-year systematic literature review
Authors: Marengo Agostino , Pagano Alessandro , Pange Jenny , Soomro Kamal Ahmed 
Published: March 2024

Purpose
This paper aims to consolidate empirical studies between 2013 and 2022 to investigate the impact of artificial intelligence (AI) in higher education. It aims to examine published research characteristics and provide insights into the promises and challenges of AI integration in academia.

Design/methodology/approach
A systematic literature review was conducted, encompassing 44 empirical studies published as peer-reviewed journal papers. The review focused on identifying trends, categorizing research types and analysing the evidence-based applications of AI in higher education.

Findings
The review indicates a recent surge in publications concerning AI in higher education. However, a significant proportion of these publications primarily propose theoretical and conceptual AI interventions. Areas with empirical evidence supporting AI applications in academia are delineated.

Research limitations/implications
The prevalence of theoretical proposals may limit generalizability. Further research is encouraged to validate and expand upon the identified empirical applications of AI in higher education.

Practical implications
This review outlines imperative implications for future research and the implementation of evidence-based AI interventions in higher education, facilitating informed decision-making for academia and stakeholders.

Originality/value
This paper contributes a comprehensive synthesis of empirical studies, highlighting the evolving landscape of AI integration in higher education and emphasizing the need for evidence-based approaches.