bluuebunny/crossref_metadata_2025_split huggingface dataset:
Halogenated Cyclic Monoterpenoids with Anti‐Biofouling Activity from the Okinawan Red Marine Algae Portieria Hornemannii
Authors: Ishigami Shinnosuke , Fukada Ryosuke , Nagasaka Genki , Tsuruta Tomoki , Nishikawa Keisuke , Sasaki Yu , Nimura Kazumi , Oshima Iori , Yamagishi Yukimasa , Morimoto Yoshiki , Kamada Takashi , Ishii Takahiro 
Published: March 2024

AbstractThe red algal genus Portieria is a prolific producer of halogenated monoterpenoids. In this study, we isolated and characterised monoterpenoids from the Okinawan red algae Portieria hornemannii. A new polyhalogenated cyclic monoterpenoid, 2(R)‐chloro‐1,6(S)‐dibromo‐3(8)(Z)‐ochtoden‐4(R)‐ol (1), along with three known monoterpenoids, (2R,3(8)E,4S,6R)‐6‐bromo‐2‐chloro‐1,4‐oxido‐3(8)‐ochtodene (2), 1‐bromo‐2‐chloroochtoda‐3(8),5‐dien‐4‐one (3), and 2‐chloro‐1‐hydroxyochtoda‐3(8),5‐dien‐4‐one (4) were isolated from the methanol extract of three populations of P. hornemannii. These compounds were characterised using a combination of spectroscopic methods and chemical synthesis, and the absolute stereochemistry of compounds 1 and 2 was determined. In addition, all isolated compounds were screened for their anti‐biofouling activity against the mussel Mytilus galloprovincialis, and 1 exhibited strong activity. Therefore, halogenated monoterpenoids have the potential to be used as natural anti‐biofouling drugs.
Split Proteins and Reassembly Modules for Biological Applications
Authors: Bae Jieun , Kim Jonghoon , Choi Jongdoo , Lee Hwiyeong , Koh Minseob 
Published: March 2024

AbstractSplit systems, modular entities enabling controlled biological processes, have become instrumental in biological research. This review highlights their utility across applications like gene regulation, protein interaction identification, and biosensor development. Covering significant progress over the last decade, it revisits traditional split proteins such as GFP, luciferase, and inteins, and explores advancements in technologies like Cas proteins and base editors. We also examine reassembly modules and their applications in diverse fields, from gene regulation to therapeutic innovation. This review offers a comprehensive perspective on the recent evolution of split systems in biological research.
Cysteine‐independent CRISPR‐Associated Protein Labeling for Presentation and Co‐delivery of Molecules Toward Genetic and Epigenetic Regulations
Authors: Tanga Sadiya , Hota Arpita , Karmakar Arkadeep , Banerjee Paramita , Maji Basudeb 
Published: March 2024

AbstractLabeling of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) associated proteins (Cas) remains an immense challenge for their genome engineering applications. To date, cysteine‐mediated bioconjugation is the most efficient strategy for labeling Cas proteins. However, introducing a cysteine residue in the protein at the right place might be challenging without perturbing the enzymatic activity. We report a method that does not require cysteine residues for small molecule presentation on the CRISPR‐associated protein SpCas9 for in vitro protein detection, probing cellular protein expression, and nuclear co‐delivery of molecules in mammalian cells. We repurposed a simple protein purification tag His6 peptide for non‐covalent labeling of molecules on the CRISPR enzyme SpCas9. The small molecule labeling enabled us to rapidly detect SpCas9 in a biochemical assay. We demonstrate that small molecule labeling can be utilized for probing bacterial protein expression in realtime. Furthermore, we coupled SpCas9’s nuclear‐targeting ability in co‐delivering the presenting small molecules to the mammalian cell nucleus for prospective genome engineering applications. Furthermore, we demonstrate that the method can be generalized to label oligonucleotides for multiplexing CRISPR‐based genome editing and template‐mediated DNA repair applications. This work paves the way for genomic loci‐specific bioactive small molecule and oligonucleotide co‐delivery toward genetic and epigenetic regulations.
PSL Chemical Biology Symposia: Recent Progress in Ferroptosis
Authors: Pereira Arthur , Baron Leeroy , Bucci Romain , Plays Marina , Bonasegale Giulia , Picard–Bernes Armel , Bibrowski Manuel , Morris Nolwenn , Marynberg Sacha , Sindikubwabo Fabien , Cañeque Tatiana , Müller Sebastian , Colombeau Ludovic , Solier Stéphanie , Bono Yannick , Gaillet Christine , Johannes Ludger , Puisieux Alain , Rodriguez Raphaël 
Published: March 2024

AbstractThis symposium is the 5th PSL (Paris Sciences & Lettres) Chemical Biology meeting (2015, 2016, 2019, 2023, 2024) held at Institut Curie. This initiative originally started at Institut de Chimie des Substances Naturelles (ICSN) in Gif‐sur‐Yvette, with a strong focus on chemistry. It was then continued at the Institut Curie (2015) covering a larger scope, before becoming the official PSL Chemical Biology meeting. This latest edition hosted around 150 participants and was focused on the burgeoning field of ferroptosis, its mechanism and implications in health and disease. While not initially planned, it was felt that the next large Ferroptosis venue (CSHA, China) would not happen before late 2024. A discussion involving Conrad, Birsoy, Ubellacker, Brabletz and Rodriguez next to lake Como in Italy sponsored by the DKFZ, prompted us to fill in this gap and to organize a Ferroptosis meeting in Paris beforehand.
Retrieval of a left appendage device from the left ventricle using a large bore steerable catheter
Authors: Abbadi Abou‐Bakr , Akodad Mariama , Horvilleur Jérôme , Neylon Antoinette 
Published: March 2024

AbstractLeft atrium appendage closure is a safe and effective therapy for patients with atrial fibrillation and high thromboembolic and hemorrhagic risks. Prosthesis embolization is a potential major complication with an incidence of 0.07%. We report a case of migration of an Amplatzer Amulet (Abbott) device that was successfully retrieved in an innovative way (4).
Nickel‐ and Palladium‐Catalyzed Copolymerizations of Norbornene with Polar α‐Olefins
Authors: Li Mingyuan , Fang Yueting , Cai Zhengguo , Eisen Moris S. 
Published: March 2024

AbstractRecent progress regarding the copolymerization of norbornene with various polar α‐olefins catalyzed by nickel and palladium catalysts is reviewed here with special attention paid to the diverse catalyst frameworks, polar monomer scopes as well as polymer microstructures. Both the influences of steric effect and electron property of ligand substituents and the type of polar monomers on the catalytic behaviors (activity, stability, polarity tolerance, etc.) and the polymer microstructures (molecular weight and distribution, comonomer contents, etc.) have been summarized. The introduction of noncyclic‐olefins, such as polar vinyl monomers, polar higher α‐olefins, and polar styrenes, into rigid cyclic‐based polynorbornene chain resulted in significant modifications on the polarity, compatibility, thermostability, processibility, transparency, and many other properties of the material, which has also been discussed in the review. The contents of this review have been classified according to the type of polar monomers involved in the norbornene copolymerization, and each section included nickel and palladium catalysts bearing different ligand structures, which will be meaningful for the development of new types of catalysts and new families of norbornene‐based cyclic olefin copolymer materials in the future.
Artificial Biocatalysis: Quo Vadis?
Authors: Ingram Aaron A. , Oike Keiko 
Published: March 2024

AbstractAstonishing progress has been achieved in unlocking new‐to‐nature biocatalysis in the past decades. The progress in protein engineering enabled research to efficiently incorporate artificial structural elements into enzyme design. Recent trends include cofactor mimetics, artificial metalloenzymes and non‐canonical amino acids. In this perspective article, we present the state‐of‐the‐art, discuss recent examples and our view on what we call artificial biocatalysis. Although these artificial systems undoubtedly increase the scope of biocatalysis, their applicability remains challenging. Fundamental questions regarding the impact of this research field are addressed in this perspective.
Catalytic Ammonia Formation in a Microreaction Chamber with Electrically Intensified Arc Plasma
Authors: Van Duc Long Nguyen , Pourali Nima , Lamichhane Pradeep , Mohsen Sarafraz Mohammad , Nghiep Tran Nam , Rebrov Evgeny , Kim Hyun‐Ha , Hessel Volker 
Published: March 2024

AbstractAmmonia (NH3) production is of global concern for today's food supply security and as future energy vector. Plasma technology can add to supply‐chain resilience of fertilizer production and improve the environmental profile using renewable energy; allowing distributed NH3 production. With the objective to provide process intensification of small‐capacity reactors for local supply, a novel micropyramid‐disk plasma reactor operated in micro‐arc mode was developed. NH3 was synthesized from N2, nitrogen, and H2, hydrogen over Ru/MCM‐41 catalyst at atmospheric pressure. The microplasma brings plasma and catalyst surface close together and intensifies the electric field. The arc plasma elevates temperature, ‘nonthermal’, releasing high‐energy free electrons, known to be effective in converting low‐reactive molecules. The study demonstrates that microplasma, with reduced electrode‐to‐electrode dimensions and a microstructured reaction environment, enhances the performance of the NH3 synthesis and opens novel process windows. This is detailed on the impact of feed ratio (N2/H2), applied voltage, frequency, electrode gap, and the flow distribution by which the gas is fed in. Optical emission spectroscopy (OES) was used to identify vibrationally and other excited species generated by the microplasma and confirms the catalyst is in symbiosis with the radicals.
Donor‐Acceptor Pyrene‐Based Covalent Organic Framework for Blue Light Photocatalytic Oxidative Coupling of Amines
Authors: Wang Linyang , Chakraborty Jeet , Deng Maojun , Sun Jiamin , Van Der Voort Pascal 
Published: March 2024

AbstractCovalent organic frameworks (COFs) with a donor‐acceptor (D−A) architecture have been investigated as prospective semiconducting materials, particularly for photocatalytic processes. The incorporation of electron donating and accepting moieties into the COF backbone creates nano heterojunctions, enhancing efficient light absorption, charge separation, and charge transport. Herein, three pyrene‐based COFs were well designed for the aerobic coupling of amines under air atmosphere with blue‐LED irradiation. Among the three COFs, the Py‐Por‐COF with D−A structure exhibits the highest photocatalytic performance and demonstrates a remarkable apparent quantum efficiency of 11.3 % at 420 nm. In addition, the Py‐Por‐COF maintains a good level of crystallinity and high yield even undergone four cycles, showcasing the exceptional potential of D−A structure COFs in the field of photocatalytic organic transformation.
Bridging the Gap Between Single Atoms, Atomic Clusters and Nanoparticles in Electrocatalysis: Hierarchical Structured Heterogeneous Catalysts
Authors: Bhalothia Dinesh , Beniwal Amisha , Kumar Saravanan Praveen , Chen Po‐Chun , Chen Tsan‐Yao 
Published: March 2024

AbstractIn the past decade, remarkable progress has been made in advancing heterogeneous single‐atom catalysts (SACs), propelled by their extraordinary properties such as unique activities, high selectivity, and efficient metal utilization. In contrast to their nanoparticle counterparts, SACs showcase distinct advantages, yet they are not exempt from limitations. A primary constraint lies in their relatively low metal content and lack of ensemble sites, potentially impacting overall catalytic activity. Stability concerns also arise, as isolated metal atoms may be prone to aggregation, oxidation, and other structural changes. To surmount these challenges, there is a burgeoning exploration of synergies between SACs and other active species, such as single atoms, atomic clusters, or nanoparticles. This approach holds promise for augmenting the efficiency of complex catalytic reactions. However, a systematic examination of their integration and potential synergy has been notably absent. This review strives to fill that gap by comprehensively exploring key interactions, complementary effects, and bifunctional roles of single atoms, atomic clusters and nanoparticles when combined in different compositions and configurations, especially for oxygen reduction reaction (ORR) and hydrogen evolution reaction (HER) applications. Additionally, the review sheds new light on the underlying catalytic mechanisms by offering insights through specific case studies. In particular, it emphasizes the dynamic nature of each active species and their interactions within these innovative catalytic entities, specifically in the context of heterogeneous electrocatalytic processes, backed by compelling in situ and operando evidence. Concluding the discussion, the review addresses the current challenges and provides a glimpse into the prospects of these integrated catalytic systems in the future. The contents can serve as a valuable guide for the design of advanced catalysts, promoting the deliberate combination and synergy of binary or multiple active species to further advance the field of catalysis with a clear roadmap and easy assessments.
Response of chickpea's yield and yield components to double inoculation with mycorrhiza and rhizobium under split application of nitrogen
Authors: Malekian Babak , Parsa Mehdi , Fallah Mohammad Hassan , Vessal Saeedreza 
Published: March 2024

AbstractThe utilization of advantageous microorganisms as a biofertilizer has gained significant importance in the agricultural industry due to their potential contribution to food safety and the sustainable cultivation of crops. To evaluate the effect of nitrogen (N) fertilizer and co‐inoculation with arbuscular mycorrhiza and rhizobium on the yield and yield component of chickpea (Cicer arietinum L.), a 2‐year field experiment was conducted in 2020–2021 at the Agricultural Research Station of Ferdowsi University of Mashhad, Iran. The experiment was designed as a randomized block trial in factorial design with three replicates. The treatments included two levels of inoculation (noninoculated and inoculated with rhizobium and mycorrhiza) and application of different levels of N fertilizer (0, 25, or 50 kg ha−1) at three growth stages (sowing, flowering, pod filling) as follows; F0 (0,0,0), F1 (25,0,0), F2 (25,25,0), F3 (25,25,25), F4 (50,0,0), F5 (50,50,0) and F6 (50,50,50), respectively. The results showed that seed inoculation and split N fertilization significantly increased yield and yield components in chickpea (Cicer arietinum L.). Seed inoculation showed the highest values for all traits studied compared to the noninoculated treatments. Moreover, among the fertilizer treatments, the highest values for plant height (41.8 cm), number of branches (9.1), number of grains per plant (17.8) and 100‐grain weight (30.4 g) from F6 through the F3 treatment, were statistically similar. The results show that the effect of inoculation is more significant when a lower amount of N fertilizer is applied. Due to the health and environmental problems associated with chemical fertilizers, double inoculation and split application of N fertilizers at lower doses can be recommended.
CO2‐based spouted fluidized bed gasification of kitchen waste: Experimental investigation with process modelling
Authors: Lokare Nilesh , Sahu Pradeep , Vairakannu Prabu 
Published: March 2024

AbstractIn the present study, kitchen waste is chosen as a solid feed for producing high‐caloric valuable syngas using CO2 as a gasifying agent. Dried kitchen waste is gasified in a spouted fluidized bed reactor for syngas production. In the process, along with syngas, a significant quantity of oil (48 wt.%) and solid char residue is obtained during pyrolysis. Further, the spouted bed process is simulated in ASPEN PLUS using various reactor modules and the calculated results is validated with the experimental data. A model using the combination of continuous stirred tank reactor (CSTR) and plug flow reactor (PFR) with recycling of gas and char fits the behaviour of spouted bed. Further, the experimental investigation of fixed and spouted bed gasification of kitchen showed that the calorific value of syngas obtained during gasification is 10.45 MJ/nm3 with 30.00 vol.% CO and 24.00 vol.% H2. Further, the effect of process parameters such as pressure, temperature, spouting gas flow rate, and feed rate of kitchen waste on overall syngas composition is evaluated using ASPEN PLUS software.
Hydrocracking of non‐edible vegetable oil and waste cooking oils for the production of light hydrocarbon fuels: A review
Authors: Ratshoshi Sharron , Mukaya Hembe Elie , Nkazi Diakanua 
Published: March 2024

AbstractThe high demand for biofuels by surfacing economies with environmental issues has resulted in a more in‐depth search for new biofuel feedstocks. Non‐edible oils have become the centre of investigation as biofuel feedstocks since they do not compete with food sources. This review evaluates hydrocracking as a biofuel production method and further elucidates different feedstocks, the effects of hydrocracking catalysts, and operating parameters to obtain optimum yields and selectivity of desired products. The bifunctional catalyst mechanism is also explained. The hydrocracking technique is found to be more advantageous than other biofuel production techniques such as transesterification and pyrolysis due to its ability to produce valuable products of better quality and with higher yields. Coke formation is one of the challenges faced by hydrocracking despite that it can be reduced by high hydrogen pressure; this will increase the cost of the entire process.
The CRP troponin test (CTT) stratifies mortality risk in patients with non‐ST elevation myocardial infarction (NSTEMI)
Authors: Brzezinski Rafael Y. , Banai Shmuel , Katz Shalhav Malka , Stark Moshe , Goldiner Ilana , Rogowski Ori , Shapira Itzhak , Zeltser David , Sasson Noa , Berliner Shlomo , Shacham Yacov 
Published: March 2024

AbstractIntroductionThe C‐reactive protein (CRP)‐troponin‐test (CTT) comprises simultaneous serial measurements of CRP and cardiac troponin and might reflect the systemic inflammatory response in patients with acute coronary syndrome. We sought to test its ability to stratify the short‐ and long‐term mortality risk in patients with non‐ST elevation myocardial infarction (NSTEMI).MethodsWe examined 1,675 patients diagnosed with NSTEMI on discharge who had at least two successive measurements of combined CRP and cardiac troponin within 48 h of admission. A tree classifier model determined which measurements and cutoffs could be used to best predict mortality during a median follow‐up of 3 years [IQR 1.8–4.3].ResultsPatients with high CRP levels ( > 90th percentile, >54 mg/L) had a higher 30‐day mortality rate regardless of their troponin test findings (16.7% vs. 2.9%, p < 0.01). However, among patients with “normal” CRP levels ( < 54 mg/L), those who had high troponin levels ( > 80th percentile, 4,918 ng/L) had a higher 30‐day mortality rate than patients with normal CRP and troponin concentrations (7% vs. 2%, p < 0.01). The CTT test result was an independent predictor for overall mortality even after adjusting for age, sex, and comorbidities (HR = 2.28 [95% CI 1.56‐3.37], p < 0.01 for patients with high troponin and high CRP levels).ConclusionsEarly serial CTT results may stratify mortality risk in patients with NSTEMI, especially those with “normal” CRP levels. The CTT could potentially assess the impact of inflammation during myocardial necrosis on the outcomes of patients with NSTEMI and identify patients who could benefit from novel anti‐inflammatory therapies.
Multi‐chaotic maps and blockchain based image encryption
Authors: Kumari Twinkle , Singh Damanpreet , Singh Birmohan 
Published: March 2024

SummaryThe vast technological developments make data transmission more frequent over networks. So, the data needs to be secured and for that reason, there is a requirement to develop an effective encryption model. This article proposes a novel image encryption model using multi‐chaotic maps and blockchain (MCBE). Chaotic maps have been used in encryption models as chaotic maps have the properties of randomness, and non‐periodicity that get utilized in improving the efficiency of an encryption model. Logistic and tent maps have been employed as multi‐chaotic maps to make the encryption process effective in this work. A logistic map has a simple structure and is easily implementable but has some drawbacks like small keyspace. Keyspace and randomness are enhanced by using a tent map along with a logistic map. The proposed MCBE methodology applies to grayscale or colored images having different file formats and sizes. Initially, in the confusion phase, the permutation of the original image is performed based on a random permutation that changes the original image pixel's position. The encryption model applies multi‐chaotic maps, row‐wise and column‐wise in the diffusion phase to promote the efficiency of the encryption model. Finally, blockchain has been implemented using the SHA‐256 hash function to obtain the encrypted image that enhances the security by increasing the keyspace, to resist brute force attacks. The evaluation performance of the MCBE has been analyzed against various attacks namely statistical attacks, differential attacks, NIST randomness test, noise, and cropping attacks. The evaluated keyspace is which has high key sensitivity with a correlation of encrypted images close to 0. Maximum achieved entropy value of 7.9998, SSIM value between original and encrypted images nearby 0 confirms the dissimilarity. The Number of Pixels Change Rate (NPCR) value of 99.62%, and the value of Unified Average Changed Intensity (UACI) value of 33.54% are within the specified standard range. The calculated correlation coefficient, NPCR, and UACI values have been compared with the existing algorithms, and the results show that the proposed MCBE methodology has better performance than the other state‐of‐the‐art methods. The experimental results indicate that the chaotic ranges generated by multi‐chaotic maps and the SHA‐256 hash function improve the keyspace and security of the encryption model confirming the efficacy of the proposed model MCBE.
A path planning method for unmanned aerial vehicle based on improved wolf pack algorithm
Authors: Jiang Hao , Yu Qizhou , Han Dan , Chen Yaqing , Li Zejun 
Published: March 2024

SummaryIn the rapidly developing field of unmanned aerial vehicle (UVA) technology, solving local optimal problems and achieving efficient smooth planning are crucial for improving the operational efficiency and safety of UVA systems. To address these needs, our study introduces a novel optimization algorithm, called IWPA‐APF, which aims to improve path planning efficiency. This algorithm is a fusion of the artificial potential field (APF) method and the improved wolf‐pack algorithm (IWPA) to solve common problems such as local optima and inefficient planning in the path planning process. The IWPA‐APF algorithm improves search efficiency and accuracy by incorporating an adaptive step size that significantly refines the key behaviors of the traditional IWPA, which is based on the wolf pack algorithm (WPA). In addition, the algorithm incorporates specific planning constraints, such as turn angles and tolerance limits, to minimize getting stuck in local optima. The process involves generating initial plans through multiple iterations of the IWPA, followed by further refinement using the method of integrating APF's repulsive force field. Simulation results show that the IWPA‐APF algorithm outperforms traditional methods, offering shorter flight distances and improved safety, thereby establishing itself as a robust solution for UAV path planning in obstacle‐rich environments.