bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of a novel potentiator of CFTR channel activity to ivacaftor in ameliorating mucostasis caused by cigarette smoke in primary human bronchial airway epithelial cells
Authors: Tanjala Adrian Constantin , Jiang Jia Xin , Eckford Paul D.W. , Ramjeesingh Mohabir , Li Canhui , Huan Ling Jun , Langeveld Gabrielle , Townsend Claire , Paone Daniel V. , Busch-Petersen Jakob , Pekhletski Roman , Tang LiPing , Raju Vamsee , Rowe Steven M. , Bear Christine E. 
Published: March 2024

AbstractBackgroundCystic Fibrosis causing mutations in the geneCFTR, reduce the activity of the CFTR channel protein, and leads to mucus aggregation, airway obstruction and poor lung function. A role for CFTR in the pathogenesis of other muco-obstructive airway diseases such as Chronic Obstructive Pulmonary Disease (COPD) has been well established. The CFTR modulatory compound, Ivacaftor (VX-770), potentiates channel activity of CFTR and certain CF-causing mutations and has been shown to ameliorate mucus obstruction and improve lung function in people harbouring these CF-causing mutations. A pilot trial of Ivacaftor supported its potential efficacy for the treatment of mucus obstruction in COPD. These findings prompted the search for CFTR potentiators that are more effective in ameliorating cigarette-smoke (CS) induced mucostasis.MethodsA novel small molecule potentiator (SK-POT1), previously identified in CFTR binding studies, was tested for its activity in augmenting CFTR channel activity using patch clamp electrophysiology in HEK-293 cells, a fluorescence-based assay of membrane potential in Calu-3 cells and in Ussing chamber studies of primary bronchial epithelial cultures. Addition of cigarette smoke extract (CSE) to the solutions bathing the apical surface of Calu-3 cells and primary bronchial airway cultures was used to model COPD. Confocal studies of the velocity of fluorescent microsphere movement on the apical surface of CSE exposed airway epithelial cultures, were used to assess the effect of potentiators on CFTR-mediated mucociliary movement.ResultsWe showed that SK-POT1, like VX-770, was effective in augmenting the cyclic AMP-dependent channel activity of CFTR. SK-POT-1 enhanced CFTR channel activity in airway epithelial cells previously exposed to CSE and ameliorated mucostasis on the surface of primary airway cultures.ConclusionTogether, this evidence supports the further development of SK-POT1 as an intervention in the treatment of COPD.
Pan-cancer molecular signatures connecting aspartate transaminase (AST) to cancer prognosis, metabolic and immune signatures
Authors: Siwo Geoffrey H. , Singal Amit G. , Waljee Akbar K. 
Published: March 2024

AbstractBackgroundSerum aspartate transaminase (sAST) level is used routinely in conjunction with other clinical assays to assess liver health and disease. Increasing evidence suggests that sAST is associated with all-cause mortality and has prognostic value in several cancers, including gastrointestinal and urothelial cancers. Here, we undertake a systems approach to unravel molecular connections between AST and cancer prognosis, metabolism, and immune signatures at the transcriptomic and proteomic levels.MethodsWe mined public gene expression data across multiple normal and cancerous tissues using the Genotype Tissue Expression (GTEX) resource and The Cancer Genome Atlas (TCGA) to assess the expression of genes encoding AST isoenzymes (GOT1 and GOT2) and their association with disease prognosis and immune infiltration signatures across multiple tumors. We examined the associations between AST and previously reported pan-cancer molecular subtypes characterized by distinct metabolic and immune signatures. We analyzed human protein-protein interaction networks for interactions between GOT1 and GOT2 with cancer-associated proteins. Using public databases and protein-protein interaction networks, we determined whether the subset of proteins that interact with AST (GOT1 and GOT2 interactomes) are enriched with proteins associated with specific diseases, miRNAs and transcription factors.ResultsWe show that AST transcript isoforms (GOT1 and GOT2) are expressed across a wide range of normal tissues. AST isoforms are upregulated in tumors of the breast, lung, uterus, and thymus relative to normal tissues but downregulated in tumors of the liver, colon, brain, kidney and skeletal sarcomas. At the proteomic level, we find that the expression of AST is associated with distinct pan-cancer molecular subtypes with an enrichment of specific metabolic and immune signatures. Based on human protein-protein interaction data, AST physically interacts with multiple proteins involved in tumor initiation, suppression, progression, and treatment. We find enrichments in the AST interactomes for proteins associated with liver and lung cancer and dermatologic diseases. At the regulatory level, the GOT1 interactome is enriched with the targets of cancer-associated miRNAs, specifically mir34a – a promising cancer therapeutic, while the GOT2 interactome is enriched with proteins that interact with cancer-associated transcription factors.ConclusionsOur findings suggest that perturbations in the levels of AST within specific tissues reflect pathophysiological changes beyond tissue damage and have implications for cancer metabolism, immune infiltration, prognosis, and treatment personalization.
Parameters that influence bipartite reporter system expression inC. elegans
Authors: Knoebel Emma , Brinck Anna , Nonet Michael L. 
Published: March 2024

AbstractThe development of bipartite reporter systems inC. eleganshas lagged by more than a decade behind its adoption in Drosophila, the other invertebrate model commonly used to dissect biological mechanisms. Here, we characterize many parameters that influence expression in recently developedC. elegansbipartite systems. We examine how DNA binding site number and spacing influence expression and characterize how these expression parameters vary in distinct tissue types. Furthermore, we examine how both basal promoters and 3’ UTR influence the specificity and level of expression. These studies provide both a framework for the rational design of driver and reporter transgenes as well as molecular and genetic tools for the creation, characterization, and optimization of bipartite system components for expression in other cell types.
Exploring the Dynamics of Social Interactions During the Juvenile Stage in a Mouse Model of Fragile X Syndrome
Authors: Bae Moon Young , Wang Bosong , Abdullah Abdullah , Ahmed Asim , Ilyas Raffay , Rasheva Veronica , Murari Kartikeya , Cheng Ning 
Published: March 2024

AbstractIntroductionFragile X Syndrome (FXS), caused by mutations in theFmr1gene, is a neurodevelopmental condition linked to cognitive and behavioral differences, including atypical interactions and heightened anxiety-like responses in social settings. Previous studies that used FXS mouse models focused mostly on adulthood but not on behaviors displayed by juvenile mice. This is especially concerning considering the extensive body of research conducted in children and adolescents with FXS, which indicates dynamic neurodevelopmental changes from childhood to adulthood. Thus, it is crucial to study how FXS affects social interactions during the juvenile stage, in both female and male mice. In this study, we compared the social behavior ofFmr1knockout (KO) mice, a model of FXS, with wild-type (WT) mice of the C57BL/6J strain.MethodsWe examined both male and female juvenile mice at postnatal day 40 (P40). Social interaction tests were carried out using two mice of the same sex and genotype, who previously had not encountered each other, and were allowed to interact freely in an empty chamber for 10 minutes. Their behaviors were recorded by an overhead camera for 10 minutes. Key social behaviors such as head, body, anogenital sniffing, and physical touch, as well as distance traveled, were analyzed with a recently developed marker-less tracking software.ResultsResults from the social interaction tests showed pronounced sex and genotype differences between juvenile females and males of both the WT and KO genotypes. Overall, sex differences were found in all the behavioral parameters that were measured. Notably, the KO females engaged in more social interaction events compared to WT females in each social behavior. On the other hand, there were no significant differences observed between the WT and KO males.DiscussionThese results highlight the importance of considering the differences that arise during the juvenile stage inFmr1KO mouse model, suggesting that early post-natal phases may be a promising window for therapeutic interventions.
Long-distance contribution to
εK
from lattice QCD
Authors: Bai Ziyuan , Christ Norman H. , Karpie Joseph M. , Sachrajda Christopher T. , Soni Amarjit , Wang Bigeng 
Published: March 2024

A lattice QCD approach to the calculation of the long-distance contributions to εK is presented. This parameter describes indirect CP violation in K→ππ decay. While the short-distance contribution to εK can be accurately calculated in terms of standard model parameters and a single hadronic matrix element, BK, there is a long-distance part which is estimated to be approximately 5% of the total and is more difficult to determine. A method for determining this small but phenomenologically important contribution to εK using lattice QCD is proposed and a complete exploratory calculation of the contribution is presented. This exploratory calculation uses an unphysical light quark mass corresponding to a 339 MeV pion mass and an unphysical charm quark mass of 968 MeV, expressed in the MS¯ scheme at 2 GeV. This calculation demonstrates that future work should be able to determine this long-distance contribution from first principles with a controlled error of 10% or less.

Published by the American Physical Society
2024
Adopting relationship trust and influence strategy to enhance green customer integration: a social exchange theory perspective
Authors: Zhou Cong , Xia Weili , Feng Taiwen 
Published: March 2024

Purpose
This study aims to explore how relationship trust and different types of influence strategy (i.e., non-coercive and coercive influence strategy) impact green customer integration (GCI), while investigating the moderating mechanisms of big data development and social capital.

Design/methodology/approach
Following hierarchical linear regression analysis, the authors examine hypothesized relationships by combining survey data from 206 Chinese manufacturers with secondary data.

Findings
The results show that relationship trust positively affects non-coercive influence strategy, while its impact on coercive influence strategy is insignificant. Non-coercive influence strategy has an inverted U-shaped impact on GCI. Furthermore, big data development flattens the inverted U-shaped relationship between non-coercive influence strategy and GCI. Conversely, social capital steepens the inverted U-shaped relationship between non-coercive influence strategy and GCI.

Practical implications
This study sheds light on managers on how to involve customers in GCI through friendly strategies that favor the involvement of customers and the willingness to develop environmentally friendly initiatives.

Originality/value
Although GCI has received widespread attention, how it can be enhanced remains unclear. These findings provide novel insights into the emerging GCI literature and complement social exchange theory.
Integrating of FDI, institutions, ICT and logistics for promoting domestic entrepreneurship: evidence from fsQCA
Authors: Luu Tien Dung , Huynh Thuy Tien , Phung Tuan Thanh 
Published: March 2024

PurposeThis paper aims to assess the relationships between foreign direct investment (FDI) and domestic entrepreneurship (DE) with the moderating role of formal institutions (FI), logistics and information communication technology (ICT) capacities.Design/methodology/approachThe study is based on unbalanced panel data of 53 countries from 2006 to 2020 at different stages of development, using a fuzzy-set qualitative comparative analysis.FindingsThe research results indicate that FDI directly affects the establishment of domestic entrepreneurship. Additionally, FDI firms via the buffer mechanism of FI, logistics and ICT development for DE. Through its adjustment to the quality of institutions, logistics and ICT infrastructure, GDP per capita determines the direction of FDI's impact on DE.Originality/valueThe study's findings grant empirical evidence and theoretical contributions to the relationship between FDI and domestic entrepreneurial development through the buffering mechanism of FI, logistics and the role of ICT.
Spatial spillover effects associated with the Sweden Democrats' growing support
Authors: Sümeghy Dávid 
Published: March 2024

AbstractResearch on the growing support of radical right parties cannot ignore the role of spatial, contextual effects and geographical proximity. One important spatial factor is the halo effect, which suggests that a radical right party is more likely to perform well in a homogeneous electoral district surrounded by ethnically diverse areas. Voter behaviour is influenced by certain neighbourhood effects, which can be spatially distant, originating from neighbouring areas. In this way, the presence of groups more inclined to support the radical right in an area can, through interaction and observations of signs or norms, modify an individual's party preference. In this paper, I model the 2018 election results of the Sweden Democrats at the level of the 6004 Swedish electoral districts using the Spatial Durbin Model specification. The spatial model shows a better fit and indicates a spillover effect of the level of education and median wealth in addition to the contextual role of ethnic diversity and its change. Changes in the proportion of non‐Europeans in neighbouring areas have had a particularly strong spillover effect, which is associated with the growing support for the Sweden Democrats.
Consumer perception and acceptance of edible packaging for various food products
Authors: Taylor Sage , Colonna Ann , Jung Jooyeoun , Gutierrez Jessica , Zhao Yanyun 
Published: March 2024

AbstractExcessive use of single‐use plastic packaging presents an imminent threat to the environment. One of the emerging solutions is using edible food packaging. However, there is lack of consumer information toward edible packaging. This study evaluated consumer attitude, acceptability, and purchase intent of three types of edible food packaging: muffin liner, cranberry pomace fruit leather wrap, and powdered drink sachet. One hundred consumers who frequently consumed muffins, strawberry fruit leather, and powdered lemonade were selected from metropolitan area of Portland, Oregon to participate in the study. The panelists were presented with the edible films and the food products with the edible packaging, information card highlighting the environmental‐friendly edible package, and were prompted with describing the sensory attributes, purchase intent, and qualities regarding the edible packaging with and without food. Overall, panelists liked the three foods with the edible packaging giving overall liking scores of 7.48, 8.06, and 7.48 for the muffin liners, edible fruit leather wraps, and powdered drink sachets, respectively, based on a 9‐point hedonic scale where 1 = dislike extremely and 9 = like extremely. When asked about hypothetical purchase intent, 64%–68% of panelists positively reacted to purchase intent and would buy all three types of edible packaging products. Based on the positive reaction from panelists, edible packaging maybe a possible solution to reducing single‐use plastic packaging in the food industry. This study can be the catalyst for further investigation of the efficacy of different applications of edible food packaging as well as consumer perceptions of eating their packaging.Practical ApplicationEdible food packaging is an emerging solution for reducing single‐use plastic waste. This study investigated consumer attitude, acceptability, and purchase intent of edible food packaging for three food packaging applications, including edible muffin liner, fruit leather wrap, and powdered drink sachet. This study demonstrated that consumers strongly agree that edible packaging would serve as an environmentally sustainable solution to single‐use plastics, and are willing to spend more to purchase these sustainable alternatives. This study provides new information toward the future development of edible packaging and consumer perceptions of eating their packaging.
Molecular sexing of birds using quantitative PCR (qPCR) of sex‐linked genes and logistic regression models
Authors: Petrou Eleni L. , Scott Laura C. , McKeeman Cherie M. , Ramey Andrew M. 
Published: March 2024

AbstractThe ability to sex individuals is an important component of many behavioural and ecological investigations and provides information for demographic models used in conservation and species management. However, many birds are difficult to sex using morphological characters or traditional molecular sexing methods. In this study, we developed probabilistic models for sexing birds using quantitative PCR (qPCR) data. First, we quantified distributions of gene copy numbers at a set of six sex‐linked genes, including the sex‐determining gene DMRT1, for individuals across 17 species and seven orders of birds (n = 150). Using these data, we built predictive logistic models for sex identification and tested their performance with independent samples from 51 species and 13 orders (n = 209). Models using the two loci most highly correlated with sex had greater accuracy than models using the full set of sex‐linked loci, across all taxonomic levels of analysis. Sex identification was highly accurate when individuals to be assigned were of species used in model building. Our analytical approach was widely applicable across diverse neognath bird lineages spanning millions of years of evolutionary divergence. Unlike previous methods, our probabilistic framework incorporates uncertainty around qPCR measurements as well as biological variation within species into decision‐making rules. We anticipate that this method will be useful for sexing birds, including those of high conservation concern and/or subsistence value, that have proven difficult to sex using traditional approaches. Additionally, the general analytical framework presented in this paper may also be applicable to other organisms with sex chromosomes.
Anthropology with a philosophical sensibility
Authors: Souza dos Santos Eraldo 
Published: March 2024

AbstractWhile philosophy and anthropology have much to say to each other, they do not always mesh in productive ways. Critically reflecting on an edited volume that seeks to bring insights from philosophy to anthropological analysis, I consider how the volume's form, while didactic, may contribute to the reproduction of power dynamics that both anthropologists and philosophers have denounced. More broadly, however, I argue that reading and engaging with philosophy can broaden anthropologists’ theoretical imagination and perception in the field, just as having an anthropological sensibility can expand the philosophical imagination and open new avenues of philosophical inquiry.
‘Not a goal, but a given’: Neonatal care participation through parents' perspective, a cross‐sectional study
Authors: Hoeben Hannah , Obermann‐Borst Sylvia A. , Stelwagen Mireille A. , van Kempen Anne A. M. W. , van Goudoever Johannes B. , van der Schoor Sophie R. D. , van Veenendaal Nicole R. 
Published: March 2024

AbstractAimTo explore parents' perspectives regarding participation in neonatal care, with focus on the family integrated care (FICare) model utilised as a tool to enhance parent–infant closeness. Additionally, we describe experiences in different architectural settings.MethodsAn online survey, categorised by four FICare pillars, was distributed through social media to parents of newborns hospitalised to Dutch neonatal wards between 2015 and 2020. Quantitative findings were summarised using descriptive statistics, while open‐ended responses were thematically analysed.ResultsAmong the 344 respondents (98% mothers), most reported feeling involved in care (315/340). However, 79% also felt separated from their infant (265/337). Irrespective of architectural settings, parents reported incomplete implementation of FICare pillars: 14% was invited to educational sessions (parent education), 51% discussed family‐specific care plans (staff education), 21% was facilitated in connecting with veteran parents (psychosocial support) and 22% received couplet‐care (environment). Although 65% of parents were invited to attend clinical rounds, 32% actively participated in decision making. Thematic analysis revealed fundamentals for feeling welcome on the ward, peer‐to‐peer support, psychosocial support and participation in clinical rounds.ConclusionOverall, parents expressed satisfaction with participation in neonatal care. However, structural implementation of FICare lacks. Regardless of architecture, expanding parent participation beyond presence requires attention.
Two‐year neurodevelopmental outcome in extremely preterm‐born children: The Austrian Preterm Outcome Study Group
Authors: Kiechl‐Kohlendorfer Ursula , Simma Burkhard , Berger Angelika , Urlesberger Berndt , Wald Martin , Haiden Nadja , Fuiko Renate , Ndayisaba Jean‐Pierre ,   The Austrian Preterm Outcome Study Group
Published: March 2024

AbstractAimThe current study determined the neurodevelopmental outcome of extremely preterm infants at 2 years of age.MethodsAll live‐born infants 23–27 weeks of gestation born between 2011 and 2020 in Austria were included in a prospective registry. Neurodevelopmental outcome at 2 years of corrected age was assessed using Bayley Scales of Infant Development for both motor and cognitive scores, along with a neurological examination and an assessment of neurosensory function.Results2378 out of 2905 (81.9%) live‐born infants survived to 2 years of corrected age. Follow‐up data were available for 1488 children (62.6%). Overall, 43.0% had no, 35.0% mild and 22.0% moderate‐to‐severe impairment. The percentage of children with moderate‐to‐severe neurodevelopmental impairment decreased with increasing gestational age and was 31.4%, 30.5%, 23.3%, 19.0% and 16.5% at 23, 24, 25, 26 and 27 weeks gestational age (p < 0.001). Results did not change over the 10‐year period. In multivariate analysis, neonatal complications as well as male sex were significantly associated with an increased risk of neurodevelopmental impairment.ConclusionIn this cohort study, a 22.0% rate of moderate‐to‐severe neurodevelopmental impairment was observed among children born extremely preterm. This national data is important for both counselling parents and guiding the allocation of health resources.
Rewilding, gender and the transformation of the Côa Valley
Authors: Bartolini Nadia , Carvalho Bárbara , May Sarah 
Published: March 2024

AbstractThis paper focuses on women's agency through their responses to a rewilding initiative. To do this, we draw on ethnographic research conducted in a rewilding landscape in the Côa Valley in Portugal. Our initial encounters in the rewilding field were all with male workers. This led us to actively seek out women's experiences, who were mainly located on the margins of rewilding. We believe these experiences cast rewilding—and its effects on rural communities—in a new light. We discuss three women's differing relationships with a rewilding initiative: Beatriz who sold land to rewilding and purchased other productive land for her son; Diana and Matilda who are running businesses alongside raising young families and envisioning futures. Their experiences highlight not only how they have developed alternative means to ensure the reproduction of the family, but also how rewilding is more than nature. We conclude that listening to voices less heard enables a better understanding of how rewilding affects people's lives and their differential access to resources.