bluuebunny/crossref_metadata_2025_split huggingface dataset:
Virulent Klebsiella pneumoniae ST11 clone carrying blaKPC and blaNDM from patients with and without COVID-19 in Brazil
Authors: Pimentel Maria Izabely Silva , Beltrão Elizabeth Maria Bispo , de Oliveira Érica Maria , Martins Lamartine Rodrigues , Jucá Moacir Batista , Lopes Ana Catarina de Souza 
Published: March 2024

Abstract

Aims
Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA, and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil.

Methods and results
All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and MLST were used. The detection of blaNDM was greater (n = 23) when compared to that of blaKPC (n = 14). The virulence genes that most occurred were fimH, entB, cps, and wabG, which are responsible for adhesins, siderophore enterobactin, capsule, and lipopolysaccharides, respectively. Among the isolates, 21 distinct genetic profiles were found by ERIC-PCR, with multiclonal dissemination. Four isolates belonged to the ST11 clone.

Conclusions
The occurrence of the ST11 is worrying as it is a high-risk clone involved in the dissemination of virulent strains throughout the world.
Fascia Iliaca Catheters for Donor Site Pain After Split-Thickness Skin Grafting for Acute Burn Injury: A Retrospective Review
Authors: Saldanha Usha , Aldwinkle Robin , Chen Amy , Raut Snehal , Penta Deepthi , Valazquez Yakelin Arroyo , Sen Soman 
Published: March 2024

Abstract
The management of acute burn pain poses significant challenges. Regional techniques have become increasingly popular in perioperative burn pain management. Continuous nerve block catheters are particularly useful for split-thickness skin grafts where donor site pain can exceed that of the burn-injured site. Donor skin is frequently harvested from the anterolateral thigh. The fascia iliaca (FI) compartment block provides blockade of both the lateral femoral cutaneous nerve and the femoral nerve, and thus, it is a useful modality for burn donor pain. Our institution initiated a protocol in which continuous FI catheters were placed in patients undergoing split-thickness skin grafting of the anterolateral thigh. This retrospective review seeks to assess the impact of this modality on postoperative pain scores and opioid requirements. Oral morphine equivalent (OME) administration was significantly lower in the FI group than the control group, starting with POD 0, which is the day of the FI catheter insertion (188 vs 327 mg, P &lt; .001). Over the next 4 postoperative days (PODs), OME administration remained lower in the FI group compared to control patients, although not statistically significant on PODs 3-4. There was a significant difference in OME administered between the FI group and the control group on POD 5 (159.5 vs 209.2 mg, P &lt; .05). Our retrospective study evaluating the role of FI catheters in burn patients undergoing split-thickness skin grafting surgery showed significantly lower opioid consumption on PODs 1-5 compared to patients without an FI catheter.
Genetic variation within a stick-insect species associated with community-level traits
Authors: Sinclair-Waters Marion , Zamorano Laura S , Gompert Zachariah , Parchman Tom , Tyukmaeva Venera , Hopkins David P , Nosil Patrik 
Published: March 2024

Abstract
Phenotypic variation within species can affect the ecological dynamics of populations and communities. Characterizing the genetic variation underlying such effects can help parse the roles of genetic evolution and plasticity in “eco-evolutionary dynamics” and inform how genetic variation may shape patterns of evolution. Here, we employ genome-wide association (GWA) methods in Timema cristinae stick insects and their co-occurring arthropod communities to identify genetic variation associated with community-level traits. Previous studies have shown that maladaptation (i.e., imperfect crypsis) of T. cristinae can reduce the abundance and species richness of other arthropods due to an increase in bird predation. Whether genetic variation that is independent of crypsis has similar effects is unknown and was tested here using genome-wide genotyping-by-sequencing data of stick insects, arthropod community information, and GWA mapping with Bayesian sparse linear mixed models. We find associations between genetic variation in stick insects and arthropod community traits. However, these associations disappear when host-plant traits are accounted for. We thus use path analysis to disentangle interrelationships among stick-insect genetic variation, host-plant traits, and community traits. This revealed that host-plant size has large effects on arthropod communities, while genetic variation in stick insects has a smaller, but still significant effect. Our findings demonstrate that (1) genetic variation in a species can be associated with community-level traits but that (2) interrelationships among multiple factors may need to be analyzed to disentangle whether such associations represent causal relationships. This work helps to build a framework for genomic studies of eco-evolutionary dynamics.
Macropinocytic cups function as signal platforms for the mTORC2-AKT pathway to modulate LPS-induced cytokine expression in macrophages
Authors: Wang Li , Sun Xiaowei , Chen Jianan , Li Yanan , He Yuxin , Wei Jinzi , Shen Zhongyang , Yoshida Sei 
Published: March 2024

Abstract
Macropinocytosis is a large-scale endocytosis process primarily observed in phagocytes as part of their cellular function to ingest antigens. Once phagocytes encounter gram-negative bacteria, the receptor proteins identify lipopolysaccharides (LPSs), which trigger radical membrane ruffles that gradually change to cup-like structures. The open area of the cups closes to generate vesicles called macropinosomes. The target bacteria are isolated by the cups and engulfed by the cells as the cups close. In addition to its ingestion function, macropinocytosis also regulates the AKT pathway in macrophages. In the current study, we report that macropinocytic cups are critical for LPS-induced AKT phosphorylation (pAKT) and cytokine expression in macrophages. High-resolution scanning electron microscope observations detailed the macropinocytic cup structures induced by LPS stimulation. Confocal microscopy revealed that AKT and the kinase molecule mTORC2 were localized in the cups. The biochemical analysis showed that macropinocytosis inhibition blocked LPS-induced pAKT. RNA sequencing, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay analyses revealed that the inhibition of macropinocytosis or the AKT pathway causes a decrease in the expression of proinflammatory cytokines interlukin-6 and interlukin-1α. Moreover, activation of the transcription factor nuclear factor κB, which regulates the cytokine expression downstream of the AKT/IκB pathway, was hindered when macropinocytosis or AKT was inhibited. These results indicate that LPS-induced macropinocytic cups function as signal platforms for the AKT pathway to regulate the cytokine expression by modulating nuclear factor κB activity in LPS-stimulated macrophages. Based on these findings, we propose that macropinocytosis may be a good therapeutic target for controlling cytokine expression.
Evaluation of a Clinical Decision Support System and an Automated Electronic Health Record Alert on Outpatient Prescribing of Cefdinir
Authors: Courtney Ashleah , Cook Gregory , Silio Margarita 
Published: March 2024

Abstract
Acute bacterial upper respiratory infections are common indications for antibiotics in pediatrics, and many prescriptions may be inappropriate. Novel approaches to outpatient antimicrobial stewardship interventions are needed. This quasi-experimental study of an order set and best practice advisory alert targeting cefdinir prescriptions demonstrated an 8.4% decrease in cefdinir prescribing (P ≤ .001).
Low scattered linear orders
Authors: Frolov Andrey , Zubkov Maxim 
Published: March 2024

Abstract
In 1998 R. Downey formulated a problem: to describe a property $P$ of classical order types, which guarantees that if $\mathcal{L}$ is a low linear order and $P$ holds for the order type of $\mathcal{L}$ then $\mathcal{L}$ is isomorphic to a computable linear order. We find a new such property $P$. Also, we give an upper bound on a complexity of an isomorphism between computable and low copies and show that this bound is sharp.
Academic History and Environment
Authors: Kerns Connor M. , Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter focuses on the Academic History and Environment section of the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version, with Autism Spectrum Addendum (ADIS/ASA): Parent Interview Schedule (PIS). The interviewer should first ask whether the child attends school or is homeschooled. If homeschooled, the interviewer should inquire why the parent/guardian decided to homeschool. The next step is to inquire if the child has difficulties going to or staying in school now or if this has happened in the past. The interviewer may also ask if there are particular subjects or activities that are particularly challenging for the child, more so than for other children their age. The last step is to inquire if the child receives any services or extra supports, such as a specialized learning plan, in or outside of school to support their learning.
Social Anxiety Disorder (Social Phobia) and Other Social Fears
Authors: Kerns Connor M. , Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter discusses the section on Social Anxiety Disorder (Social Phobia) and Other Social Fears in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version, with Autism Spectrum Addendum (ADIS/ASA): Parent Interview Schedule (PIS). This section offers some guidelines and special considerations for differentiating symptoms of social anxiety from behavioral difficulties secondary to Autism Spectrum Disorder (ASD). Fears of being laughed at, rejected, or excluded by others may be realistic, appropriate, and protective for some children. Clinicians should assess for fears of negative social evaluation that are excessive even in light of the child’s social history and social ability. Meanwhile, not all cases of social avoidance or low social motivation are due to social fears—children may avoid social interaction for other reasons. Clinicians should consider how fears of negative evaluation or other aspects of social interaction add to the child’s social difficulties rather than attributing all social challenges to anxiety. They should also be careful to assess social fears rather than a sensitivity to the noise associated with social gatherings or groups. In some case, parents may not endorse fears of negative evaluation because their child has limited awareness of thoughts or feelings of others at this stage in their development. Children with this profile can nonetheless experience problematic fears and anxiety in social situations.
Specific Phobias
Authors: Kerns Connor M. , Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter details the section on Specific Phobias in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version, with Autism Spectrum Addendum (ADIS/ASA): Parent Interview Schedule (PIS). This section provides some guidelines and special considerations for differentiating symptoms of specific phobia from sensory sensitivities that are characteristic of Autism Spectrum Disorder (ASD) and for capturing idiosyncratic phobias that may be present. Children on the autism spectrum may be highly sensitive to certain sounds, sights, tastes, touches, or smells. Specific phobias occur when a child’s fear of exposure to a specific thing is excessive and interfering. It is the anxiety that is problematic in phobia rather than the sensory sensitivity per se. As an example, a child with a sensitivity to loud sounds might cover their ears, cry, and try to get away from the loud sound. A child with a phobia of loud sounds would avoid places and situations where loud sounds might occur, have trouble focusing in class because they are worrying about a fire drill, or even refuse to go to school due to their fears of a drill. When assessing specific phobia that may have a sensory component, clinicians should be sure to assess symptoms of and rate the interference associated with anxiety, not only the sensory sensitivity itself.
Fears Related to Focused Interests
Authors: Kerns Connor M. , Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter looks at the section on Fears Related to Focused Interests in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version, with Autism Spectrum Addendum (ADIS/ASA): Parent Interview Schedule (PIS). Focused Interest Fears may be a distinct aspect of Generalized Anxiety Disorder (GAD) in youth on the autism spectrum if they occur alongside these symptoms. If GAD is not present, these symptoms may be represented by a diagnosis of Other Specified Anxiety Disorder if it is deemed clinically appropriate and meaningful for treatment planning. It is important to measure how much the problem interferes with the child’s friendships, has caused problems at school or at home, and has stopped the child from doing the things they would like to do. This can be done through the Feelings Thermometer.
Introduction
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter discusses the first part of the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version: Parent Interview Schedule (PIS). It begins with a brief introduction and explanation of the purpose of the interview, obtaining a brief description of the child's presenting complaint and ascertaining the reasons for seeking professional help at this time. If both parents are present and give discrepant responses, the interviewer may wish to specify their responses with an “M” (for the mother) or “F” (for the father) in the appropriate places. Throughout the interview, some questions will represent symptoms or criteria for diagnostic categories and are followed by “Yes,” “No,” and “Other” responses. For some criteria, a certain number of symptoms must be obtained before those criteria can be assigned. The interviewer should also explain the Feelings Thermometer and the concept of interference.
Specific Phobia
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter looks at the section on Specific Phobia in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version: Parent Interview Schedule (PIS). For each situation in Question 1b that the parent endorsed with a fear rating of 4 or greater, the interviewer should inquire about the child's avoidance behavior. If no items received an interference rating of 4 or greater, the interviewer may skip to the section on Panic Disorder. To meet the diagnostic criteria for Specific Phobia, the parent must have endorsed a fear of one or more objects or situations with a rating of 4 (Some) or greater; avoidance or endurance of the specific situation with intense fear or anxiety; and interference in normal routine, academic functioning, or social activities or relationships (rating of 4 or greater). The duration must be 6 months or longer.
Persistent Depressive Disorder (Dysthymia)
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter highlights the section on Persistent Depressive Disorder (Dysthymia) in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version: Parent Interview Schedule (PIS). If the parent responds “Yes” to Question 1a, and “No” to Question 1b (parent reports past dysthymia but denies current episode), the interviewer may skip to the section on Disruptive Mood Dysregulation Disorder. If the parent responds “Yes” to Question 1b, the interviewer should continue to evaluate present episode. The next step is to measure interference through the Feelings Thermometer. If the child meets the criteria for Persistent Depressive Disorder (Dysthymia), the interviewer should inquire about age of onset.
Bipolar Disorder
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter details the section on Bipolar Disorder in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version: Parent Interview Schedule (PIS). The interview should ensure that the child's elevated mood or irritability is not accounted for by other disorders such as Oppositional Defiant Disorder (ODD), attention deficit hyperactivity disorder (ADHD), or substance use disorder. If the parent endorsed a Major Depressive Episode, the interviewer should inquire whether these symptoms occurred during the same 2-week period. If criteria are met for a Manic or Hypomanic Episode, the interviewer should consider a diagnosis of Bipolar Disorder. If the child's symptoms do not meet the full criteria for a Manic or Hypomanic Episode, the interviewer should consider a diagnosis of Bipolar Disorder, NOS. These children may have typical bipolar symptoms but not the DSM-5 required duration. In addition, these children may be characterized by severe irritability, aggressiveness, agitation, and/or mood lability. There may also be more rapid cycling of mood.
Elimination Disorders
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter focuses on the Elimination Disorders section of the Anxiety and Related Disorders Interview Schedule for DSM-5, Child Version: Parent Interview Schedule (PIS). The interviewer should first inquire whether, after reaching age 5, the child has had the problem of wetting their pants or bed either during the day or night. If the child is over 5 years of age, has no physical disorder, the parent has responded “Yes” to Question 1, and the problem has occurred at least twice weekly for three consecutive months or caused significant distress or interference in functioning, then a diagnosis of Enuresis is warranted. The interviewer should then inquire whether, since reaching age 4, the child has had the problem of soiling their pants or passing their stools/feces into inappropriate places, such as on the floor rather than in the toilet. If the child is over 4 years of age, has no physical disorder, the parent has responded “Yes” to Question 1, and the problem has occurred at least weekly for three consecutive months (Question 2), history and/or physical exam will determine if there is evidence of overflow incontinence and constipation to assign the DSM subtype of Encopresis.
Interpersonal Relationships
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter highlights the module on Interpersonal Relationships in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child and Parent Version: Child Interview Schedule (CIS). In this section, the interviewer should inquire for all manners in which the child/adolescent keeps in contact with others. If the child/adolescent does not text or use any social media, the interviewer may inquire about any family rules or limitations placed on these outlets. The next step is to inquire about social activities, whether or not the child/adolescent participates in extracurricular activities. It is also important to inquire if the child/adolescent has ever been ignored, bullied, or made fun of. If the child/adolescent answers “yes” to any of these situations, the interviewer should ask what they do when these situations happen. If the child/adolescent answers “no,” the interviewer may skip to the module on Social Anxiety Disorder (Social Phobia).
Selective Mutism
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter details the Selective Mutism module in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child and Parent Version: Child Interview Schedule (CIS). If the child has not responded verbally during the interview, it might be necessary to encourage them to use the Feelings Thermometer or to nod yes or no to answer the questions. It may be necessary to encourage the child to write their responses to the inquiry, if they do not speak. Diagnosis of Selective Mutism requires a failure to speak in selected situations, such as school, despite speaking in other situations; interference in functioning; and duration of at least 1 month of not speaking beyond the first month of school. If the child’s responses suggest Selective Mutism, the interviewer may continue with the Interference Rating.
Tic Disorders
Authors: Albano Anne Marie , Silverman Wendy K. 
Published: March 2024

Abstract
This chapter looks at the Tic Disorders module in the Anxiety and Related Disorders Interview Schedule for DSM-5, Child and Parent Version: Child Interview Schedule (CIS). With appropriate sensitivity, the interviewer should physically demonstrate motor tics when inquiring about their presence. If duration of tics is less than 1 year, the interviewer should consider a diagnosis of Other Specified Tic Disorder or Unspecified Tic Disorder and skip to Vocal Tics. Symptoms must have persisted for at least 1 year, although they could wax and wane in frequency, and onset of symptoms must be prior to age 18. It is very important to distinguish interference due to tics from distress due to other comorbid problems such as Attention-Deficit/Hyperactivity Disorder (ADHD) or Obsessive-Compulsive Disorder (OCD). If only motor tics or vocal tics are present and they occurred many times a day, for less than 1 year, the interviewer should consider a diagnosis of Provisional Tic Disorder.
Profession-Specific Roles in Palliative Care
Authors: Saks Naomi Tzril , Wallace Cara L. , Donesky DorAnne , Milic Michelle M. 
Published: March 2024

Abstract
Although interprofessional collaboration in palliative care is heralded as paramount to successful education, practice, and outcomes, few practical models guide collaboration among team members. Minimal literature outlines and compares the backgrounds and defines the advanced practice roles of the core members of the team. In this chapter the authors introduce a road map to interprofessional collaboration rooted in the framework of transdisciplinary team collaboration and operationalized in the Generalist-Specialist Model of Interprofessional Practice in Palliative Care. They also review the backgrounds and role descriptions of the social worker, chaplain, nurse, and physician on specialty palliative care teams. The chapter concludes with a brief discussion of challenges to role identity and development that impact synergy and recommendations for the growth of sustainable models of interprofessional teamwork.
Professional Identity and Interprofessional Tension
Authors: Kwok Ian B. , Sumser Bridget 
Published: March 2024

Abstract
Tension and conflict arise in all professional teams. Interprofessional palliative care teams face unique challenges as a result of the profound, complex nature of the work. Furthermore, healthcare systems are founded on cultural systems of power, historically marginalizing certain team members, and often contributing to tension and conflict within the palliative care team. This chapter identifies common sources of conflict, explored through a sociological framework with an emphasis on the concepts of professional identity, organizational structures, power dynamics, and intersectionality. It concludes with an exploration of best practices and strategies for naming and negotiating tensions and related team distress within the context of a palliative care team.