bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of kinin receptors on eosinophils: comparison of asthmatic patients and healthy subjects
Authors: Bertram Cornelia M , Misso Neil L , Fogel-Petrovic Mirjana , Figueroa Carlos D , Foster Paul S , Thompson Philip J , Bhoola Kanti D 
Published: November 2008

AbstractEosinophils contribute to asthmatic airway inflammation by releasing cysteinyl leukotrienes (cysLT) and other inflammatory mediators, and bradykinin (BK) induces bronchoconstriction in asthmatic patients. The aims of this study were to investigate kinin receptor expression on eosinophils of asthmatic and healthy subjects and to assess the effects of kinin stimulation on eosinophils, which were isolated from peripheral blood of asthmatic (n=27) and healthy subjects (n=14). Kinin B1 and B2 receptors (B1R and B2R, respectively) and mRNA expression were investigated by quantitative confocal microscopy, flow cytometry, and RT-PCR. Intracellular Ca2+ was assessed by live-cell fluorescence confocal microscopy. Production of cysLT and eosinophil migration in response to BK and Lys-des[Arg9]-BK were assessed. Eosinophils expressed kinin B1R and B2R mRNA and proteins. Quantitative immunofluorescence analysis indicated that expression of B1R and B2R proteins was significantly greater in eosinophils of asthmatic patients compared with those of nonasthmatic subjects. However, kinin B1R and B2R mRNA expression did not differ significantly between these groups. Expression of kinin B1R and mRNA was decreased in patients using high doses of inhaled corticosteroids and in eosinophils treated with a corticosteroid in vitro. Kinin B1 and B2 agonists up-regulated expression of their respective receptors but did not increase intracellular Ca2+ or the production of cysLT or enhance eosinophil migration significantly. Up-regulation of kinin receptor expression in eosinophils of asthmatic patients may be a consequence of inflammation, whereby enhanced release of kinin peptides has a positive-feedback effect on kinin receptor expression. Importantly, anti-inflammatory corticosteroids down-regulated the expression of the kinin B1R.
The green seaweed Ulva rigida as a bioindicator of metals (Zn, Cu, Pb and Cd) in a low-salinity coastal environment
Authors: Boubonari Theodora , Malea Paraskevi , Kevrekidis Theodoros 
Published: November 2008

Abstract
The capacity of Ulva rigida as a bioindicator of Zn, Cu, Pb and Cd contamination in oligohaline coastal waters was investigated. Metal concentrations in U. rigida, water and sediments and several environmental variables were measured seasonally at four stations in the coastal section of the Evros River Delta, Aegean Sea. All four metals in sediments had a significant local variation; in particular, they decreased with increasing distance from the fluvial end-member of Evros River. Mean Zn, Cu, Pb and Cd concentrations in U. rigida were 61.0, 22.4, 6.1 and 1.1 μg g-1 dry weight, respectively. This macroalga, compared to other co-occurring macroalgal species, had a high metal accumulation capacity, mainly of Cu and Cd. Local patterns of Zn, Cu and Pb in U. rigida were similar to those in sediments, while that of Cd was not; moreover, Cu and Pb in U. rigida correlated positively with their sediment contents, suggesting that this seaweed satisfies the basic prerequisite for a bioindicator of these elements in oligohaline environments. No metal in U. rigida displayed a significant seasonal variation, and there was no significant variation in ambient concentrations. A negative correlation between Zn in U. rigida and water salinity was found, probably due to both a higher metal burden in the freshwater flowing in the study area and to a decrease in Zn uptake with increasing salinity; Cd in the macroalga correlated negatively with water column ammonium.
Derivation and Characterization of Canine Embryonic Stem Cell Lines with In Vitro and In Vivo Differentiation Potential
Authors: Vaags Andrea K. , Rosic-Kablar Suzana , Gartley Cathy J. , Zheng Yan Zhen , Chesney Alden , Villagómez Daniel A.F. , Kruth Stephen A. , Hough Margaret R. 
Published: November 2008

Abstract
Embryonic stem cells (ESCs) represent permanent cell lines that can be maintained in an undifferentiated state. In an environment that induces differentiation, they form derivatives of the three embryonic germ layers: mesoderm, ectoderm, and endoderm. These characteristics give ESCs great potential for both basic research and clinical applications in the areas of regenerative medicine and tissue engineering. The establishment of ESCs from large animals that model human diseases is of significant importance. We describe the derivation of permanent canine cell lines from preimplantation-stage embryos. Similar to human ESCs, canine ESCs expressed OCT3/4, NANOG, SOX2, SSEA-3, SSEA-4, TRA-1–60, TRA-1–81, and alkaline phosphatase, whereas they expressed very low levels of SSEA-1. They maintained a normal karyotype and morphology typical of undifferentiated ESCs after multiple in vitro passages and rounds of cryopreservation. Plating cells in the absence of a feeder layer, either in attachment or suspension culture, resulted in the formation of embryoid bodies and their differentiation to multiple cell types. In vivo, canine ESCs gave rise to teratomas comprising cell types of all three embryonic germ layers. These cells represent the first pluripotent canine ESC lines with both in vitro and in vivo differentiation potential and offer the exciting possibility of testing the efficacy and safety of ESC-based therapies in large animal models of human disease.
Enforced Expression of Mixl1 During Mouse ES Cell Differentiation Suppresses Hematopoietic Mesoderm and Promotes Endoderm Formation
Authors: Lim Sue Mei , Pereira Lloyd , Wong Michael S. , Hirst Claire E. , Van Vranken Benjamin E. , Pick Marjorie , Trounson Alan , Elefanty Andrew G. , Stanley Edouard G. 
Published: November 2008

Abstract
The Mixl1 gene encodes a homeodomain transcription factor that is required for normal mesoderm and endoderm development in the mouse. We have examined the consequences of enforced Mixl1 expression during mouse embryonic stem cell (ESC) differentiation. We show that three independently derived ESC lines constitutively expressing Mixl1 (Mixl1C ESCs) differentiate into embryoid bodies (EBs) containing a higher proportion of E-cadherin (E-Cad)+ cells. Our analysis also shows that this differentiation occurs at the expense of hematopoietic mesoderm differentiation, with Mixl1C ESCs expressing only low levels of Flk1 and failing to develop hemoglobinized cells. Immunohistochemistry and immunofluorescence studies revealed that Mixl1C EBs have extensive areas containing cells with an epithelial morphology that express E-Cad, FoxA2, and Sox17, consistent with enhanced endoderm formation. Luciferase reporter transfection experiments indicate that Mixl1 can transactivate the Gsc, Sox17, and E-Cad promoters, supporting the hypothesis that Mixl1 has a direct role in definitive endoderm formation. Taken together, these studies suggest that high levels of Mixl1 preferentially allocate cells to the endoderm during ESC differentiation.
Autonomous underwater vehicles (AUVs) and investigations of the ice–ocean interface in Antarctic and Arctic waters
Authors: Dowdeswell J.A. , Evans J. , Mugford R. , Griffiths G. , McPhail S. , Millard N. , Stevenson P. , Brandon M.A. , Banks C. , Heywood K.J. , Price M.R. , Dodd P.A. , Jenkins A. , Nicholls K.W. , Hayes D. , Abrahamsen E.P. , Tyler P. , Bett B. , Jones D. , Wadhams P. , Wilkinson J.P. , Stansfield K. , Ackley S. 
Published: November 2008

AbstractLimitations of access have long restricted exploration and investigation of the cavities beneath ice shelves to a small number of drillholes. Studies of sea-ice underwater morphology are limited largely to scientific utilization of submarines. Remotely operated vehicles, tethered to a mother ship by umbilical cable, have been deployed to investigate tidewater-glacier and ice-shelf margins, but their range is often restricted. The development of free-flying autonomous underwater vehicles (AUVs) with ranges of tens to hundreds of kilometres enables extensive missions to take place beneath sea ice and floating ice shelves. Autosub2 is a 3600 kg, 6.7 m long AUV, with a 1600 m operating depth and range of 400 km, based on the earlier Autosub1 which had a 500 m depth limit. A single direct-drive d.c. motor and five-bladed propeller produce speeds of 1–2 m s−1. Rear-mounted rudder and stern-plane control yaw, pitch and depth. The vehicle has three sections. The front and rear sections are free-flooding, built around aluminium extrusion space-frames covered with glass-fibre reinforced plastic panels. The central section has a set of carbon-fibre reinforced plastic pressure vessels. Four tubes contain batteries powering the vehicle. The other three house vehicle-control systems and sensors. The rear section houses subsystems for navigation, control actuation and propulsion and scientific sensors (e.g. digital camera, upward-looking 300 kHz acoustic Doppler current profiler, 200 kHz multibeam receiver). The front section contains forward-looking collision sensor, emergency abort, the homing systems, Argos satellite data and location transmitters and flashing lights for relocation as well as science sensors (e.g. twin conductivity–temperature–depth instruments, multibeam transmitter, sub-bottom profiler, AquaLab water sampler). Payload restrictions mean that a subset of scientific instruments is actually in place on any given dive. The scientific instruments carried on Autosub are described and examples of observational data collected from each sensor in Arctic or Antarctic waters are given (e.g. of roughness at the underside of floating ice shelves and sea ice).
Planimetric and volumetric glacier changes in the Khumbu Himal, Nepal, since 1962 using Corona, Landsat TM and ASTER data
Authors: Bolch Tobias , Buchroithner Manfred , Pieczonka Tino , Kunert André 
Published: November 2008

AbstractMultitemporal space imagery from 1962 (Corona KH-4), 1992 (Landsat TM), 2001 and 2005 (Terra ASTER) was used to investigate the glacier changes in the Khumbu Himal, Nepal. The ice coverage in the investigation area decreased by about 5% between 1962 and 2005, with the highest retreat rates occurring between 1992 and 2001. The debris coverage increased concomitantly with the decrease in total glacier area. The clean-ice area decreased by >10%. Digital terrain model (DTM) generation from the early Corona KH-4 stereo data in this high-relief terrain is time-consuming, and the results still contain some elevation errors. However, these are minor in the snow-free areas with gentle slopes. Thus comparison of the surfaces of the debris-covered glacier tongues based on the Corona DTM and an ASTER DTM is feasible and shows the downwasting of the debris-covered glaciers. The highest downwasting rates, more than 20 m (>0.5 m a−1), can be found near the transition zone between the active and the stagnant glacier parts of the debris-covered glacier tongues. The downwasting is lower, but still evident, in the active ice areas and at the snout with thick debris cover. All investigated debris-covered glaciers in the study area show similar behaviour. The estimated volume loss for the investigated debris-covered glacier tongues is 0.19 km3.
A sensitivity study of factors influencing warm/thin permafrost in the Swiss Alps
Authors: Luetschg Martina , Lehning Michael , Haeberli Wilfried 
Published: November 2008

AbstractAlpine permafrost distribution is controlled by a great number of climatic, topographic and soil-specific factors, including snow cover, which plays a major role. In this study, a one-dimensional finite-element numerical model was developed to analyze the influence of individual snow-specific and climatic factors on the ground thermal regime. The results indicate that the most important factor is snow depth. Snow depths below the threshold value of 0.6 m lack sufficient insulation to prevent low atmospheric temperatures from cooling the soil. The date of first winter snow insulation and variations in mean annual air temperature (MAAT) are also shown to be important. Delays in early-winter snow insulation and in summer snow disappearance are shown to be of approximately equal significance to the ground thermal conditions. Numerical modelling also indicates that the duration of effective thermal resistance of snow cover governs the slope of the linear dependency between MAAT and mean annual ground surface temperatures (MAGST). Consequently, the most direct effect of a long-term rise in air temperatures on ground temperatures is predicted under a thin snow cover with early snowmelt in spring and/or where a large change in the date of total snowmelt occurs, in response to atmospheric warming.
Incorporating sustainability criteria into credit risk management
Authors: Weber Olaf , Scholz Roland W. , Michalik Georg 
Published: November 2008

AbstractDoes a commercial debtor's economic, environmental and social performance in terms of sustainability affect its credit risk rating? Does adding criteria aimed at assessing a lender's environmental, social or sustainability practices provide added value to traditional financial rating criteria? Many analyses have reported that a correlation exists between companies' environmental and their financial performance. We checked out the assertion that it ‘pays to be sustainable’ by analyzing the role that criteria pertaining to sustainability and environmental orientation play in the commercial credit risk management process. Our results show that sustainability criteria can be used to predict the financial performance of a debtor and improve the predictive validity of the credit rating process. We conclude that the sustainability a firm demonstrates influences its creditworthiness as part of its financial performance. Copyright © 2008 John Wiley & Sons, Ltd and ERP Environment.
Executive dysfunction as a risk marker for substance abuse: The role of impulsive personality traits
Authors: Dolan Sara L. , Bechara Antoine , Nathan Peter E. 
Published: November 2008

AbstractThe relationships between family history of substance use, executive functions, impaired decision making, and current substance dependence are the focus of this study. Thirty‐eight substance‐dependent inpatients were compared with 30 community controls on performance on the Wisconsin Card Sorting Test, Trail‐Making Test‐B, the Stroop Color Word Test, the Wais‐II Digit Span, and the Iowa Gambling Task. Recent alcohol use, depressive symptoms, and impulsivity were also assessed. As hypothesized, individuals with substance dependence exhibited poorer executive functioning. Family history status was modestly related to impaired performance on tests designed to measure the integrity of the dorsolateral prefrontal cortex. In particular, substance‐dependent, family history positive individuals performed less well on the Wisconsin Card Sorting Test than substance‐dependent individuals without a family history. Digit Span performances were worse among family history positive controls when compared with family history negative controls. Impulsive personality traits—specifically, difficulty thinking before acting (Urgency)—were related, as hypothesized, to executive functioning. Results indicate that family history status confers an increased risk of impairment beyond that conferred by recent substance abuse, and that impulsive personality traits are related to performance on tests of executive functions. Implications for criminality are discussed. Copyright © 2008 John Wiley & Sons, Ltd.
ATP‐Noncompetitive Inhibitors of CDK–Cyclin Complexes
Authors: Orzáez Mar , Gortat Anna , Mondragón Laura , Bachs Oriol , Pérez‐Payá Enrique 
Published: November 2008

AbstractProgression through the cell division cycle is controlled by a family of cyclin‐dependent kinases (CDKs), the activity of which depends on their binding to regulatory partners (cyclins A–H). Deregulation of the activity of CDKs has been associated with the development of infectious, neurodegenerative, and proliferative diseases such as Alzheimer's, Parkinson's, or cancer. Most cancer cells contain mutations in the pathways that control the activity of CDKs. This observation led this kinase family to become a central target for the development of new drugs for cancer therapy. A range of structurally diverse molecules has been shown to inhibit the activity of CDKs through their activity as ATP antagonists. Nevertheless, the ATP binding sites on CDKs are highly conserved, limiting the kinase specificity of these inhibitors. Various genetic and crystallographic approaches have provided essential information about the mechanism of formation and activation of CDK–cyclin complexes, providing new ways to implement novel research strategies toward the discovery of new, more effective and selective drugs. Herein we review the progress made in the development of ATP‐noncompetitive CDK–cyclin inhibitors.
C6‐Unsubstituted Pyrazolo[3,4‐d]pyrimidines Are Dual Src/Abl Inhibitors Effective against Imatinib Mesylate Resistant Chronic Myeloid Leukemia Cell Lines
Authors: Santucci Maria Alessandra , Corradi Valentina , Mancini Manuela , Manetti Fabrizio , Radi Marco , Schenone Silvia , Botta Maurizio 
Published: November 2008

AbstractDocking simulations were used to predict the most favorable interaction between the T315I mutated form of Abl (invariably associated with resistance to the tyrosine kinase inhibitor imatinib mesylate, IM) and C6‐unsubstituted and substituted pyrazolo[3,4‐d]pyrimidines previously found to be dual Src/Abl inhibitors. Two C6‐unsubstituted (1 and 2) and eight C6‐substituted compounds (3–10) were selected and assayed for their effects on the Ba/F3 cell line transducing the wild‐type p210Bcr–Abl construct, which is IM‐sensitive, or three of the most common mutations associated with IM resistance in vivo (T315I, Y253F, and E255K), and driven to drug resistance by saturating doses of IL‐3 or by the expression of the Bcr–Abl construct coding for the p185 protein of acute lymphoblastic leukemia. Compounds 1 and 2 were active against all cell lines assayed (LD50 range: 0.7–4.3 μM), whereas C6‐substituted compounds exhibited lower activity (LD50∼8 μM for compound 3 toward the T315I mutant). Notably, 1 and 2 were also effective toward the T315I mutation, which is insensitive to dual Src/Abl inhibitors. The cytotoxic effects of 1 and 2 on IM‐sensitive and IM‐resistant Ba/F3 cells were attributable, at least in part, to their pro‐apoptotic activity. Taken together, such findings suggest that C6‐unsubstituted pyrazolo[3,4‐d]pyrimidines may represent useful inhibitors to target IM‐resistant chronic myeloid leukemia.
Mathematical Modelling of Cation Contamination in a Proton‐exchange Membrane
Authors: Weber A. Z. , Delacourt C. 
Published: November 2008

AbstractTransport phenomena in an ion‐exchange membrane containing both H+ and K+ are described using multicomponent diffusion equations (Stefan–Maxwell). A model is developed for transport through a Nafion 112 membrane in a hydrogen‐pump setup. The model results are analysed to quantify the impact of cation contamination on cell potential. It is shown that limiting current densities can result due to a decrease in proton concentration caused by the build‐up of contaminant ions. An average cation concentration of 30 to 40% is required for appreciable effects to be noticed under typical steady‐state operating conditions.
Economic development and fluctuations in earnings inequality in the very long run: The evidence from Latin America 1900–2000
Authors: FitzGerald Valpy 
Published: November 2008

AbstractLatin America has the most unequal income distribution of any region in the world, yet its historical causes are poorly understood. This paper reports the first exploratory attempt to compute income distributions for the five leading Latin American economies for the whole 20th century. The methodology produces estimates of earnings dispersion for four skill groups over 1900–2000, which can be used to generate the familiar Gini coefficients. Large fluctuations in dispersion over time are found: countering claims of stability since the colonial past in the recent economic institutions literature; but supporting the findings of economic historians and development economists. An estimation model (reflecting the impact of international trade, labour quality and macroeconomic imbalances) explains the data reasonably well, with all three sets of drivers proving significant, although the measured effects are different across the five countries. The paper concludes that the skill composition of the workforce not only underpins long run trends in income distribution; but also conditions inequality fluctuations in response to exogenous shocks. Copyright © 2008 John Wiley & Sons, Ltd.
Solifluction processes on permafrost and non‐permafrost slopes: results of a large‐scale laboratory simulation
Authors: Harris Charles , Kern‐Luetschg Martina , Murton Julian , Font Marianne , Davies Michael , Smith Fraser 
Published: November 2008

AbstractWe present results of full‐scale physical modelling of solifluction in two thermally defined environments: (a) seasonal frost penetration but no permafrost, and (b) a seasonally thawed active layer above cold permafrost. Modelling was undertaken at the Laboratoire M2C, Université de Caen‐Basse Normandie, Centre National de la Recherche Scientifique, France. Two geometrically similar slope models were constructed using natural frost‐susceptible test soil. In Model 1 water was supplied via a basal sand layer during freezing. In Model 2 the basal sand layer contained refrigerated copper tubing that maintained a permafrost table. Soil freezing was from the top down in Model 1 (one‐sided freezing) but from the top down and bottom up (two‐sided freezing) in Model 2. Thawing occurred from the top down as a result of positive air temperatures. Ice segregation in Model 1 decreased with depth, but in Model 2, simulated rainfall led to summer frost heave associated with ice segregation at the permafrost table, and subsequent two‐sided freezing increased basal ice contents further. Thaw consolidation in Model 1 decreased with depth, but in Model 2 was greatest in the ice‐rich basal layer. Soil shear strain occurred during thaw consolidation and was accompanied by raised pore water pressures. Displacement profiles showed decreasing movement rates with depth in Model 1 (one‐sided freezing) but ‘plug‐like’ displacements of the active layer over a shearing basal zone in Model 2 (two‐sided active layer freezing). Volumetric transport rates were approximately 2.8 times higher for a given rate of surface movement in the permafrost model compared with the non‐permafrost model. Copyright © 2008 John Wiley & Sons, Ltd.