bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum vitamins and homocysteine levels in autoimmune liver disease: A systematic review and meta‐analysis
Authors: Li Jiahuan , Tian Shan , Ci Bai , Xi Yuwen , Deng Xiaoling 
Published: April 2024

AbstractObjectiveVitamins and homocysteine (Hcy) are involved in liver metabolism and related to the pathogenesis of autoimmune liver disease (AILD), but consensus is lacking. This study aims to systematically summarize relevant evidence to clarify the association of serum vitamins and Hcy levels with AILD.MethodsThe English and Chinese literature was searched until August 29, 2023. Studies were included if they were observational studies of investigating serum vitamins and Hcy levels in patients with AILD and their healthy comparisons. Quality assessment was performed by using the Newcastle–Ottawa Scale, and a meta‐analysis was conducted using ReviewManager 5.3. The protocol was registered in the international prospective register of systematic reviews (PROSPERO), with registration number CRD42023455367.ResultsA total of 25 case–control studies comprising 3487 patients (1673 patients and 1814 healthy controls) were included for analysis. There were 548 autoimmune hepatitis (AIH) cases, 1106 primary biliary cholangitis (PBC) cases, and 19 primary sclerosing cholangitis (PSC) cases. We found that serum A and E were decreased in both AIH and PBC/PSC; but vitamin C was reduced only in patients with PBC, not AIH. In addition, decreased content of 25(OH)D3 was found in both AIH and PBC. However, levels of 25(OH)D did not differ between the patients and controls, and were independent of disease types and the country. Only one study that met the inclusion criteria reported vitamin B6, B9, B12, and Hcy changes, and found that vitamin B6 and B9 were significantly decreased in patients with PBC, while serum vitamin B12 and Hcy levels were significantly elevated in them. One eligible study each confirmed a reduction in plasma vitamin K1 and 1,25(OH)2D3 in patients with PBC.ConclusionMost vitamins are deficient in AILD, so appropriate vitamin supplementation should be necessary. Further studies with larger sample sizes are needed to validate these findings.
Comparison of effectiveness and safety of molnupiravir versus sotrovimab for COVID‐19: A systematic review and meta‐analysis
Authors: Amani Bahman , Amani Behnam 
Published: April 2024

AbstractBackground and AimThis systematic review and meta‐analysis aimed to compare the effectiveness and safety of molnupiravir and sotrovimab in the treatment of patients with coronavirus disease 2019 (COVID‐19).MethodsCochrane Library, Web of Science, PubMed, medRxiv, and Google Scholar were systematically searched to identify relevant evidence up to December 2023. The risk of bias was assessed using the risk of bias in nonrandomized studies of interventions tool. Data were analyzed using Comprehensive Meta‐Analysis (CMA).ResultsOur search identified and included 13 studies involving 16166 patients. The meta‐analysis revealed a significant difference between the molnupiravir and sotrovimab groups in terms of the mortality rate (odds ratio [OR] = 2.07, 95% confidence interval [CI]: 1.16, 3.70). However, no significant difference was observed between the two groups in terms of hospitalization rate (OR = 0.71, 95% CI: 0.47, 1.06), death or hospitalization rate (OR = 1.51, 95% CI: 0.81, 2.83), and intensive care unit admission (OR = 0.59, 95% CI: 0.07, 4.84). In terms of safety, molnupiravir was associated with a higher incidence of adverse events (OR = 1.67, 95% CI: 1.21, 2.30).ConclusionThe current findings indicate that sotrovimab may be more effective than molnupiravir in reducing the mortality rate in COVID‐19 patients. However, no statistical difference was observed between the two treatments for other effectiveness outcomes. The certainty of evidence for these findings was rated as low or moderate. Further research is required to provide a better comparison of these interventions in treating COVID‐19 patients.
A nested case‐control study of untargeted plasma metabolomics and lung cancer among never‐smoking women within the prospective Shanghai Women's Health Study
Authors: Rahman Mohammad L. , Shu Xiao‐Ou , Jones Dean P. , Hu Wei , Ji Bu‐tian , Blechter Batel , Wong Jason Y. Y. , Cai Qiuyin , Yang Gong , Gao Yu‐Tang , Zheng Wei , Rothman Nathaniel , Walker Douglas , Lan Qing 
Published: April 2024

AbstractThe etiology of lung cancer in never‐smokers remains elusive, despite 15% of lung cancer cases in men and 53% in women worldwide being unrelated to smoking. Here, we aimed to enhance our understanding of lung cancer pathogenesis among never‐smokers using untargeted metabolomics. This nested case‐control study included 395 never‐smoking women who developed lung cancer and 395 matched never‐smoking cancer‐free women from the prospective Shanghai Women's Health Study with 15,353 metabolic features quantified in pre‐diagnostic plasma using liquid chromatography high‐resolution mass spectrometry. Recognizing that metabolites often correlate and seldom act independently in biological processes, we utilized a weighted correlation network analysis to agnostically construct 28 network modules of correlated metabolites. Using conditional logistic regression models, we assessed the associations for both metabolic network modules and individual metabolic features with lung cancer, accounting for multiple testing using a false discovery rate (FDR) < 0.20. We identified a network module of 121 features inversely associated with all lung cancer (p = .001, FDR = 0.028) and lung adenocarcinoma (p = .002, FDR = 0.056), where lyso‐glycerophospholipids played a key role driving these associations. Another module of 440 features was inversely associated with lung adenocarcinoma (p = .014, FDR = 0.196). Individual metabolites within these network modules were enriched in biological pathways linked to oxidative stress, and energy metabolism. These pathways have been implicated in previous metabolomics studies involving populations exposed to known lung cancer risk factors such as traffic‐related air pollution and polycyclic aromatic hydrocarbons. Our results suggest that untargeted plasma metabolomics could provide novel insights into the etiology and risk factors of lung cancer among never‐smokers.
A randomized controlled trial to evaluate the effects of an early postnatal lifestyle modification program on diet, adiposity and metabolic outcome in mothers with gestational diabetes mellitus
Authors: Tsoi Kit Ying , Chan Ruth C. M. , Zhang Cuilin , Tam Wing Hung , Ma Ronald C. W. 
Published: April 2024

AbstractObjectiveThe aim of the present study was to evaluate the effectiveness of a 12‐month early postnatal lifestyle intervention program in women with gestational diabetes in a recent pregnancy.MethodsThis study was a prospective randomized intervention study conducted at a diabetes center in Hong Kong. Chinese women aged 18–45 years, who developed gestational diabetes mellitus (GDM) in their most recent pregnancy, were invited. Eligible women were randomized in 1:1 ratio at baseline (6–12 weeks postpartum), to standard care or lifestyle intervention (diet and physical activity) groups for 12 months. A standardized biochemistry assessment including oral glucose tolerance test, blood lipids, complete blood count, renal and liver functions, were measured at baseline and at 12‐month. Anthropometry assessment and lifestyle questionnaire were performed at various timepoints.ResultsA total of 103 women were randomized at baseline and a total of 79 women (standard care, n = 39, intervention, n = 40) completed the assessment. After the 12‐month study period, women in the intervention group had significantly lower energy intake (intervention, −497.6 ± 488.3 kcal; standard, −222.0 ± 390.0 kcal, P < 0.01) compared to the standard care group, and a trend towards greater weight reduction (intervention, −0.93 ± 4.68 kg; standard, −0.01 ± 3.12 kg, P = 0.36).ConclusionThe lifestyle intervention implemented within 3 months postpartum appeared to promote postpartum weight loss. The early postnatal lifestyle intervention program may provide an opportunity to reduce the long‐term risk of diabetes in this high‐risk population.
LncRNA RP11‐301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR‐17‐5P/ATOH8 axis
Authors: Hao Shuning , Zuo Feifei , Zhang Hongmin , Wang Ying , Huang Liwen , Ma Fenghui , Song Tiefeng , Zhang Tongcun , Ren Xuejun , Wang Nan 
Published: April 2024

AbstractLong noncoding RNAs (LncRNAs) play essential roles in regulating gene expression in various biological processes. However, the function of lncRNAs in vascular smooth muscle cell (VSMC) transformation remains to be explained. In this work, we discover that a new bone marrow protein (BMP) signaling target, lncRNA RP11‐301G19.1, is significantly induced in BMP7‐treated VSMCs through lncRNA microarray analysis. Addition of BMP signaling inhibitor LDN‐193189 attenuates the expression of ACTA2 and SM‐22α, as well as the mRNA level of RP11‐301G19.1. Furthermore, lncRNA RP11‐301G19.1 is critical to the VSMC differentiation and is directly activated by SMAD1/9. Mechanistically, knocking down of RP11‐301G19.1 leads to the decrease of ATOH8, another BMP target, while the forced expression of RP11‐301G19.1 reactivates ATOH8. In addition, miR‐17‐5p, a miRNA negatively regulated by BMP‐7, contains predicted binding sites for lncRNA RP11‐301G19.1 and ATOH8 3′UTR. Accordingly, overexpression of miR‐17‐5p decreases the levels of them. Together, our results revealed the role of lncRNA RP11‐301G19.1 as a miRNA sponge to upregulate ATOH8 in VSMC phenotype transformation.
B cells and atherosclerosis: A HIV perspective
Authors: Obare Laventa M. , Bonami Rachel H. , Doran Amanda C. , Wanjalla Celestine N. 
Published: April 2024

AbstractAtherosclerosis remains a leading cause of cardiovascular disease (CVD) globally, with the complex interplay of inflammation and lipid metabolism at its core. Recent evidence suggests a role of B cells in the pathogenesis of atherosclerosis; however, this relationship remains poorly understood, particularly in the context of HIV. We review the multifaceted functions of B cells in atherosclerosis, with a specific focus on HIV. Unique to atherosclerosis is the pivotal role of natural antibodies, particularly those targeting oxidized epitopes abundant in modified lipoproteins and cellular debris. B cells can exert control over cellular immune responses within atherosclerotic arteries through antigen presentation, chemokine production, cytokine production, and cell–cell interactions, actively participating in local and systemic immune responses. We explore how HIV, characterized by chronic immune activation and dysregulation, influences B cells in the context of atherosclerosis, potentially exacerbating CVD risk in persons with HIV. By examining the proatherogenic and antiatherogenic properties of B cells, we aim to deepen our understanding of how B cells influence atherosclerotic plaque development, especially within the framework of HIV. This research provides a foundation for novel B cell‐targeted interventions, with the potential to mitigate inflammation‐driven cardiovascular events, offering new perspectives on CVD risk management in PLWH.
Targeted and untargeted metabolomic profiles in wild rabbit does (Oryctolagus cuniculus) of different breeding states (pregnant and lactating)
Authors: Marín‐García Pablo Jesús , Rouco Carlos , Llobat Lola , Larsen Torben , Hedemann Mette Skou 
Published: April 2024

AbstractEcological nutrition aims to unravel the extensive web of nutritional links that drives animals in their interactions with their ecological environments. Nutrition plays a key role in the success of European wild rabbit (Oryctolagus cuniculus) and could be affected by the breeding status of the animals and reflected in the metabolome of this species. As nutritional needs are considerably increased during pregnancy and lactation, the main objective of this work was to determine how the breeding status (pregnant and lactating) of European wild rabbit does affects nutritional requirements and their metabolome (using targeted and untargeted metabolomics), aiming to find a useful biomarker of breeding status and for monitoring nutritional requirements. To address this gap, 60 wild European rabbits were studied. Animals were divided according to their breeding status and only pregnant (n = 18) and lactating (n = 11) rabbit does were used (n = 29 in total). The body weight and length of each animal were analyzed. The relative and absolute chemical composition of the gastric content and whole blood sample were taken, and targeted and untargeted metabolomics were analyzed. As a main result, there were no differences in biometric measurements, gastric content, and targeted metabolomics, except for live weight and nonesterified fatty acids (NEFA), as pregnant animals showed higher live weight (+12%; p = 0.0234) and lower NEFA acid levels (−46%; p = 0.0262) than lactating females. Regarding untargeted metabolomics, a good differentiation of the metabolome of the two breeding groups was confirmed, and it was proven that pregnant animals showed higher plasmatic levels of succinic anhydride (3.48 more times; p = 0.0236), succinic acid (succinate) (3.1 more times; p = 0.0068) and propionic acid (3.98 more times; p = 0.0121) than lactating animals. However, lactating animals showed higher levels of N‐[(3a,5b,7b)‐7‐hydroxy‐24‐oxo‐3‐(sulfoxide) cholan‐24‐yl]‐Glycine (cholestadien) (2.4 more times; p < 0.0420), 4‐maleyl‐acetoacetate (MAA) (3.2 more times; p < 0.0364) and irilone (2.2 more times; p = 0.0451) than pregnant animals, any of these metabolites could be used as a potential biomarker. From these results, it can be concluded that the most notable changes were observed in the metabolome of individuals, with most of the changes observed being due to energy and protein mobilisation.
Parkinsonism originates in a discrete secondary and dystonia in a primary motor cortical‐basal ganglia subcircuit
Authors: Kumbhare Deepak , Weistroffer George , Goyanaga Sofia , Huang Zi Ling , Blagg Jacob , Baron Mark S. 
Published: April 2024

AbstractAlthough manifesting contrasting phenotypes, Parkinson's disease and dystonia, the two most common movement disorders, can originate from similar pathophysiology. Previously, we demonstrated that lesioning (silencing) of a discrete dorsal region in the globus pallidus (rodent equivalent to globus pallidus externa) in rats and produced parkinsonism, while lesioning a nearby ventral hotspot‐induced dystonia. Presently, we injected fluorescent‐tagged multi‐synaptic tracers into these pallidal hotspots (n = 36 Long Evans rats) and permitted 4 days for the viruses to travel along restricted connecting pathways and reach the motor cortex before sacrificing the animals. Viral injections in the Parkinson's hotspot fluorescent labeled a circumscribed region in the secondary motor cortex, while injections in the dystonia hotspot labeled within the primary motor cortex. Custom probability mapping and N200 staining affirmed the segregation of the cortical territories for Parkinsonism and dystonia to the secondary and primary motor cortices. Intracortical microstimulation localized territories specifically to their respective rostral and caudal microexcitable zones. Parkinsonian features are thus explained by pathological signaling within a secondary motor subcircuit normally responsible for initiation and scaling of movement, while dystonia is explained by abnormal (and excessive) basal ganglia signaling directed at primary motor corticospinal transmission.
Increased glutamatergic neurotransmission between the retinohypothalamic tract and the suprachiasmatic nucleus of old mice
Authors: Herrera‐Zamora J. Manuel , Osuna‐Lopez Fernando , Reyes‐Méndez Miriam E. , Valadez‐Lemus Ramon E. , Sánchez‐Pastor Enrique A. , Navarro‐Polanco Ricardo A. , Moreno‐Galindo Eloy G. , Alamilla Javier 
Published: April 2024

AbstractCircadian rhythms synchronize to light through the retinohypothalamic tract (RHT), which is a bundle of axons coming from melanopsin retinal ganglion cells, whose synaptic terminals release glutamate to the ventral suprachiasmatic nucleus (SCN). Activation of AMPA–kainate and NMDA postsynaptic receptors elicits the increase in intracellular calcium required for triggering the signaling cascade that ends in phase shifts. During aging, there is a decline in the synchronization of circadian rhythms to light. With electrophysiological (whole‐cell patch‐clamp) and immunohistochemical assays, in this work, we studied pre‐ and postsynaptic properties between the RHT and ventral SCN neurons in young adult (P90–120) and old (P540–650) C57BL/6J mice. Incremental stimulation intensities (applied on the optic chiasm) induced much lesser AMPA–kainate postsynaptic responses in old animals, implying a lower recruitment of RHT fibers. Conversely, a higher proportion of old SCN neurons exhibited synaptic facilitation, and variance–mean analysis indicated an increase in the probability of release in RHT terminals. Moreover, both spontaneous and miniature postsynaptic events displayed larger amplitudes in neurons from aged mice, whereas analysis of the NMDA and AMPA–kainate components (evoked by RHT electrical stimulation) disclosed no difference between the two ages studied. Immunohistochemistry revealed a bigger size in the puncta of vGluT2, GluN2B, and GluN2A of elderly animals, and the number of immunopositive particles was increased, but that of PSD‐95 was reduced. All these synaptic adaptations could be part of compensatory mechanisms in the glutamatergic signaling to ameliorate the loss of RHT terminals in old animals.
An optimized approach for increasing lesion size in temperature‐controled setting using a catheter with a surface thermocouple and efficient irrigation
Authors: Takigawa Masateru , Yamaguchi Junji , Goya Masahiko , Iwakawa Hidehiro , Yamamoto Tasuku , Amemiya Miki , Ikenouchi Takashi , Negishi Miho , Kawamura Iwanari , Goto Kentaro , Shigeta Takatoshi , Nishimura Takuro , Takamiya Tomomasa , Tao Susumu , Ohuchi Katsuhiro , Suzuki Sayaka , Miyazaki Shinsuke , Sasano Tetsuo 
Published: April 2024

AbstractBackgroundWe explore an optimized approach for increasing lesion size using a novel ablation catheter with a surface thermocouple and efficient irrigation in a temperature‐control setting.MethodsWe conducted radiofrequency applications at various power levels (35 W, 40 W, and 45 W), contact forces (CFs, 10 g/20 g), and durations (60 s/120 s/180 s) in perpendicular/parallel catheter orientations, with normal saline irrigation (NS‐irrigation) and Half NS‐irrigation (HNS‐irrigation) in an ex‐vivo model (Step 1). In addition, we performed applications (35 W/40 W/45 W for 60 s/120 s/180 s in NS‐irrigation and 35 W/40 W for 60 s/120 s/180 s in HNS‐irrigation) in four swine (Step 2), evaluating lesion characteristics and the occurrence of steam pops.ResultsIn Step 1, out of 288 lesions, we observed 47 (16.3%) steam pops, with 13 in NS‐irrigation and 34 in HNS‐irrigation (p = .001). Although steam pops were mostly observed with the most aggressive setting (45 W/180 s, 54%) with NS‐irrigation, they happened in less aggressive settings with HNS irrigation. Lesion size significantly increased with longer‐duration ablation but not with HNS‐irrigation. The optimal %impedance‐drop cutoff to predict steam pops was 20% with a negative‐predictive‐value (NPV) = 95.1% including NS‐ and HNS‐irrigation groups, and 22% with an NPV = 96.1% in NS‐irrigation group. In Step 2, similar to the ex‐vivo model, lesion size significantly increased with longer‐duration ablation but not with HNS‐irrigation. Steam pops were absent with NS‐irrigation (0/35) even with the largest %impedance‐drop reaching 31% at 45 W/180 s. All steam pops were observed with HNS‐irrigation (6/21, 29%). The optimal %impedance‐drop cutoff predicting steam pops was 24% with an NPV = 96.3% including both NS‐ and HNS‐irrigation groups.ConclusionsRather than using HNS‐irrigation, very long‐duration of radiofrequency applications up to 45 W/180 s may be recommended to safely and effectively increase lesion dimensions using this catheter with NS‐irrigation.
When competitive rewards create obsessions with bottom‐line outcomes: A social interdependence theory perspective of the mediating role of bottom‐line mentality
Authors: Mawritz Mary B. , Greenbaum Rebecca L. , Deng Yingli , Rosikiewicz Blythe L. , Farro Andrea C. , Mitchell Mark 
Published: April 2024

SummaryWe rely on social interdependence theory to examine bottom‐line mentality (BLM) as a motivational state that explains the effects of competitive rewards on oppositional actions in the forms of decreases in interpersonal organizational citizenship behavior (OCBI) and increases in social undermining. In line with social interdependence theory, we also examine learning goal orientation as a motivational attribute that can weaken these effects, suggesting that in comparison to employees low on learning goal orientation, employees high on the attribute are less likely to respond to their BLMs (that result from competitive rewards) with dysfunctional behaviors, because these employees will pursue their bottom‐line outcomes with an innate motivation to learn. We tested our hypotheses with three studies: an experimental study, a multisource, field study from a U.S. corporation, and a diverse, multisource, time‐lagged field study. Results from the experimental study and first field study revealed that BLM mediated the negative relationship between competitive rewards and OCBI; the second field study replicated these findings and demonstrated support for our full moderated mediation model. We provide theoretical and empirical support for the notion that BLM can serve as a motivational state that explains the effects of competition on workplace behaviors and learning goal orientation influences these effects.
Porcine computational modeling to investigate developmental dysplasia of the hip
Authors: Yu Chia‐Yu , Mannen Erin M. , Lujan Trevor J. , Uzer Gunes , Upasani Vidyadhar , Edmonds Eric W. , Fitzpatrick Clare K. 
Published: April 2024

AbstractWhile it is well‐established that early detection and initiation of treatment of developmental dysplasia of the hip (DDH) is crucial to successful clinical outcomes, research on the mechanics of the hip joint during healthy and pathological hip development in infants is limited. Quantification of mechanical behavior in both the healthy and dysplastic developing joints may provide insight into the causes of DDH and facilitate innovation in treatment options. In this study, subject‐specific three‐dimensional finite element models of two pigs were developed: one healthy pig and one pig with induced dysplasia in the right hindlimb. The objectives of this study were: (1) to characterize mechanical behavior in the acetabular articular cartilage during a normal walking cycle by analyzing six metrics: contact pressure, contact area, strain energy density, von Mises stress, principal stress, and principal strain; and (2) to quantify the effect on joint mechanics of three anatomic abnormalities previously identified as related to DDH: variation in acetabular coverage, morphological changes in the femoral head, and changes in the articular cartilage. All metrics, except the contact area, were elevated in the dysplastic joint. Morphological changes in the femoral head were determined to be the most significant factors in elevating contact pressure in the articular cartilage, while the effects of acetabular coverage and changes in the articular cartilage were less significant. The quantification of the pathomechanics of DDH in this study can help identify key mechanical factors that restore normal hip development and can lead to mechanics‐driven treatment options.