bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Grand (Ducal) Finale
Authors: Cantell Sointu 
Published: April 2024

The San Lorenzo Basilica in Florence is renowned for its Medici-infused Renaissance program, but there is a lesser-known phase of the Medici family's history in the basilica that unfolded in the 18th century. Between 1738 and 1743, Anna Maria Luisa de' Medici, the last surviving member of the Medici line, oversaw extensive architectural and decorative interventions within the San Lorenzo complex. Her efforts to maintain the cultural heritage of her ancestors were heavily shaped by the imminent end of the Medici bloodline and the difficult transition of the Grand Duchy of Tuscany to the House of Habsburg-Lorraine.
This article explores Anna Maria's motivations for her elaborate renovation projects in the basilica, with a particular focus on two of her most notable commissions: Vincenzo Meucci's cupola fresco in the nave of the basilica and the new bell tower designed by architect Ferdinando Ruggieri. These two projects, the only ones completed by Anna Maria before her death, demonstrate her ambitions within the San Lorenzo and help to uncover several underlying premises for their conceptions.
Identification of SNPs in the MAP4K4 Gene Coding Sequence and Their Association with Mastitis Susceptibility/Resistance in Nili Ravi Buffaloes
Authors: Dhillon Sehajpal Singh , Kaur Simarjeet , Mukhopadhyay C.S , Dash Shakti Kant 
Published: April 2024

Background: The Nili-Ravi water buffalo (Bubalus bubalis) stands out as the premier domestic dairy animal, renowned for its exceptional milk production capabilities. Its significance in agriculture is underscored by its remarkable efficiency in converting crop by-products into valuable resources, coupled with its ability to thrive and maintain productivity amidst changing climatic conditions. An instrumental player in inflammation regulation, the Mitogen-Activated Protein Kinase 4 (MAP4K4) gene holds a pivotal role. The primary objective of the presented research communication was to investigate the potential correlation between specific single nucleotide polymorphisms (SNPs) found across the entirety of the MAP4K4 gene’s sequence and the susceptibility of Nili Ravi buffaloes to mastitis. Methods: A total of 40 whole blood samples were collected having mastitis positive (n=20) and an equal number (20) of healthy, normal counterparts. From total 40 samples, RNA extracted, cDNA was synthesized and the PCR products generated by employing specific primers were meticulously purified using a gel purification kit after agarose gel electrophoresis. Subsequently, the MAP4K4 gene was amplified and subjected to sequencing procedures. The chromatogram data obtained from sequencing were subjected to homology analysis using the Finch-TV tool. This scrutiny unveiled the presence of a total of seven single nucleotide polymorphisms (SNPs). To elucidate the potential connection between the identified SNPs and the animals’ susceptibility or resistance to mastitis, a chi-square test was employed. Result: In conclusion, Identified seven SNPs at locations 173, 226, 252, 329, 400, 410 and 459. In 3 SNPs, values were found to be less than 0.05 and in 4 SNPs values were found to be greater than 0.05. This confirmed the possibility of association of the MAP4K4 gene with the first 3 determined SNPs. The current genome association study showed the potential correlation between these significant polymorphisms and the incidence of mastitis in Nili Ravi buffaloes. As a result, the MAP4K4 gene may be an ideal candidate gene for dairy buffalo selection against mastitis and the discovered polymorphisms may one day serve as potential genetic markers.
Pathomorphological and Molecular Studies of Respiratory Mannheimiosis in Goats
Authors: Mazumder Amdedul Islam , Begum Shameem Ara , Buragohain Mridusmrita , Gogoi Sophia M. , Regon Manjula , Hazorika Mousumi , Hazarika Girin , Dutta Biswajit 
Published: April 2024

Background: Mannheimia is a Gram-negative, facultative anaerobe, non-spore forming and non-motile bacteria belongs to the family Pasteurellaceae. Caprine bacterial pneumonia is most often caused by Mannheimia haemolytica (previously known as Pasteurella haemolytica) and Pasteurella multocida which are more frequently associated with the outbreak of acute pneumonia and death of goats, particularly young once. Mannhemia is an opportunistic pathogen that particularly inhabits the nasopharynx and tonsils of goat and sheep. The present study was aimed to study Mannheimiosis in Guwahati, Assam in order to diagnose and control the disease among goats. Methods: The present investigation was conducted during the year 2021-2022 in Guwahati, Assam. A total of 51 pneumonic lung samples were collected from nearby slaughter houses and during necropsy at Department of Pathology, C.V.Sc., A.A.U., Khanapara, Guwahati-22. All the samples were subjected to primary isolation in blood agar and MacConkey's agar and further confirmation was made using PCR. Result: Out of total 51 pneumonic lung samples examined seven (13.72%) cases were found positive for Mannheimia haemolytica infection where 5 cases were found to be associated with other respiratory pathogens. Visible gross lesions observed during necropsy were broncho-interstitial pneumonia, suppurative pneumonia and fibrinous pneumonia with hemorrhagic tracheitis. Histopathological examination revealed presence of characteristic oat cells, intravascular thrombosis, fibrin balls, oedema, necrosis and polymorphonuclear cell infiltration in the lungs. Bacteriological culture for isolation and identification of Mannheimia haemolytica showed β haemolytic zone on MLA plates. Confirmatory diagnosis was made using PCR targeting 16s rRNA gene with amplicon size 1500 bp and Lkt gene with amplicon size 206 bp. The phylogenetic analysis of 16s rRNA gene from positive sample showed percent identity of above 97% with other strains of Mannheimia haemolytica present in NCBI website. The variability in the lesions observed in the present study it can be concluded that a mixed infection with reduced resistance of the animals contributed to the development of pneumonia rather than Mannheimia haemolytica alone and PCR can be used as reliable technique for confirmatory diagnosis of respiratory mannheimiosis.
The Pelvic (floor) Reminders (to) Explore Perinatal (women’s) Acceptability (of) Reminders (to) Exercise (PREPARE) study: protocol for a feasibility trial
Authors: Harper Rosie C , Armstrong Matthew , Sheppard Sally , Stewart Carly , Clark Carol J 
Published: April 2024

Abstract
Background
Pelvic Floor Muscle Dysfunction (PFMD) is a condition that affects up to one in three women at some point in their lifetime and can typically be traced back to the perinatal period. Supervised Pelvic Floor Muscle Training (PFMT) is the gold standard of treatment for symptoms of PFMD however women commonly forget to complete PFMT. ‘Nudges’ are a behavioural intervention that can be targeted to certain populations and can be integrated in existing reminder features in mobile apps. No study has tested this novel digital intervention to encourage perinatal women to complete PFMT. The overall aim of this feasibility trial is to explore adherence to PFMT in those who receive targeted digital nudges and a mobile app compared to those who receive a leaflet.
Methods
A feasibility study will explore acceptability of digital PFMT nudges amongst perinatal women. Seventy-five women over 26 weeks pregnant will be invited to participate in the study. Women who consent to participating will be randomised to either the intervention or control group. Those in the intervention group will receive a weekly digital PFMT nudge and a PFMT mobile app until they are four months postnatal. Those in the control group will receive a PFMT leaflet. Participants will have three follow-ups at 9, 21 and 31 weeks corresponding to the appropriate gestational and post-natal phase. Both quantitative and qualitative data will be collected. Quantitative data will be collected at each follow up and will include the EQ5D5L, ICIQ-UI SF, EARS, the Broome Pelvic Muscle Self-Efficacy Scale, self-recorded PFMT adherence logs as well as in-app adherence. Qualitative data will be collected through interviews of between five to ten participants at the end of the study. One open-ended question on the acceptability of the intervention at each follow up will be asked.
Discussion
This feasibility trial will attempt to better understand perinatal women’s acceptability of the novel intervention and study processes provide practical and relevant solutions that can be taken forward in a randomised controlled trial.
Identifying and prioritizing the post-corona consequences of COVID-19 in Iranian cities
Authors: Faraji Seyed J. , Arash Majid , Arman Mohadeseh 
Published: April 2024

Abstract

In the current era, which is the world of increasing interactions and the growth of rapprochement with other societies, the influence of societies on each other has increased dramatically. This issue has well been illustrated by the COVID-19 epidemic, a disease that has spread through the world in the shortest possible period of time and it is still transmitting. One of the countries having been affected by this epidemic is Iran, whose urban communities are severely affected. The aim of this current research is to identify the consequences of the COVID-19 epidemic in the post-corona era in cities in Iran. To achieve this goal, a combination of both quantitative and qualitative methods, the thematic analysis methods, Fuzzy Delphi, ISM, and Micmac analysis have been used. The findings of research on the consequences of this epidemic in the post-corona era in metropolitan areas in Iran have been classified as 6 pervasive concepts, 20 theme organizers, and 91 basic themes, which have been extracted from the interview. twenty organizer themes were evaluated by fuzzy Delphi and the results are included in the article. Meanwhile, the ISM Method evaluated 6 Pervasive themes (Social-cultural, Educational, Economical, Health and sanitary, Political administrative, Technological). Based on that, two political and socio-cultural themes were placed in the third layer, and the economic theme was in the second layer, and finally, the educational and health- sanitary themes were in the first layer.
Virus-associated hemophagocytic syndrome caused by influ B and varicella-zoster virus co-infection
Authors: Chen Xiaojun , Wang Yuyu , Yan Dongning 
Published: April 2024

Abstract

Background:Varicella (chicken pox), which caused by the varicella-zoster virus(VZV), is usually self-limiting and benign. However, VZV can lead to significant and serious complications, especially in immunocompromised patients or accompanied by other pathogens infection. HPS caused by varicella-zoster and influ B virus co-infection is rare.
Case presentation:A 15-years-old boy was admitted to our hospital because of general rash, severe back and low back pain. CT scan revealed pneumonia in left upper lobe. Initial blood tests showed normal blood WBC and PLT, mild liver dysfunction, enhanced D-dimer and myocardial enzyme. However, after 4-days treatment of acyclovir, antibiotics, and analgesic therapy, his pain did not relieved and fever developed. At the same time, hyperferritinemia, abrupt reduction on blood WBC and PLT count were observed. Virus-associated hemophagocytic syndrome (VHAS)was confirmed.Then, intravenous drip dexamethasone(10mg/day) and gamma globulin(10mg/day) were administrated. He recovered completely at last.
Conclusions:Disseminated Varicella which was accompanied by influenza B virus and bacteria infection is infrequent. Disseminated varicella may cause significant morbidity and even mortality in immunocompromised patients. Hemophilic syndrome induced by duel-virus and bacteria infection is limited and usually fetal.Anti-bacterial therapy, early identification of haemophilus syndrome and timely administration of glucocorticoids and gamma globulin are the key links of treatments.
Efficacy of balloon catheter dilation treatment in children with phimosis in China
Authors: An Bing , Li Jia-kuan , Wang Jun , Zhang Man , Chen Xu-lin , Zheng Cheng-zhong , Liu Gang 
Published: April 2024

Abstract

Background
Circumcision has been the traditional method for the treatment of children with phimosis. Recently, a new treatment technique for children's phimosis is presented, which minimizes the repairing time and the postoperative complications, and also maintains the intact physical foreskin appearance. Existing studies encourage the use of this new technique, Balloon Catheter Dilation Treatment (BCDT), for the treatment of phimosis. We aim to investigate the efficacy and safety of BCDT in children with different types of phimosis.
Methods
A study was performed in children who were referred to our hospital for possible phimosis, between July 2015 and March 2018. All children were treated with either BCDT or conventional circumcision (CC). children were then reassessed for a one-month follow-up. A total of 3,671 outpatients were assigned to receive CC(n = 1175) or BCDT (n = 2496). Patient characteristics, operative time, visual analogue scale (VAS) of pain, cosmetic result, and complications were recorded.
Results
Significant differences were observed in complications (including the rate of blood loss, pain, edema, incarceratiom, dysuria), with p-value < 0.01 between the two groups of children given different treatment. There were significant differences in the VAS scores of pain at two hours after surgery (p-value < 0.01). Children who underwent BCDT were significantly more satisfied with the cosmetic results (p-value < 0.01).
Conclusion
BCDT is a safe, effective, and minimally invasive technique for children with phimosis. Compared with CC, BCDT have the advantages of a faster postoperative healing and a better effect on wound cosmetics.
Trial registration:
The consent to participate is not applicable because it is a retrospective study.
Effects of dietary interventions and different types of physical activity in sedentary obese adults: a network meta-analysis
Authors: Wang Chongpeng , Liu Xi , Chow Shengchih , Zhang Ping 
Published: April 2024

Abstract

Obesity is one of the most important health problems facing society today. Body mass index is an important indicator used to measure the degree of obesity. Sedentary adults are one of the main groups suffering from obesity. This network meta-analysis (NMA) was designed to compare the effects of different training methods and diets on body mass index results in sedentary adult obese patients. PubMed, EBSCO and Web of Science were searched to identify the following:(1) randomized controlled trials (RCTs); (2) the study population means ages 18–65 with overweight and sedentary;(3) Body Mass Index (BMI) > 25 kg/m2;(4) No other diseases that interfere with the weight loss effect. All articles are written in English and published from January 1991 to January 2024. Random-effects NMA models were fitted in a frequentist approach. GRADE framework was used to assess the certainty of evidence. Forty-one RCTs with 3442 participants with obesity were included in this review. The group without dietary control and exercise was used as the baseline. The BMI index was used as the unit. The results of point estimation showed that in the case of dietary control, the best effect was combined training(combine CET and RT, 1.4, 95% CI: 0.53 to 2.3) and the worst was interval training (0.79, 95% CI:-0.86 to 2.4). In the absence of dietary control, the best effect was Hybridge-type training (1.1, 95% CI: 0.23 to 2.0), and the worst was the control group (as the baseline).
Sustainability in The Arctic: A Bibliometric Analysis
Authors: Ahmed Fatma , Poelzer Greg , Zapata Oscar 
Published: April 2024

Abstract

This paper examines the literature on the Sustainability in the Arctic region, using a bibliometric analysis of 213 English-language articles published between 1980 and 2022 exploiting Bibliometrix, an R package. To find relevant literature using the Web of Science (WOS) database, we searched for documents using mesh terms based on the query of two terms, “Arctic & Sustainability”. We used the Boolean operator “AND” to combine the two terms and the Boolean operator "OR" to include synonyms of the terms. The articles retrieved were authored by 724 researchers, published in 98 journals, representing 132 countries, and growing at 5.08% annually. The findings reveal that a substantial portion of the Arctic sustainability literature placed significant emphasis on the examination of climate change, adaptation, and vulnerabilities affecting local communities. Furthermore, the more recent publications in this field concentrate predominantly on exploring perceptions and governance.
Tumor immune dynamics and long-term clinical outcome of stage IIIA NSCLC patients treated with neoadjuvant chemoimmunotherapy
Authors: Schmid Dominic , Sobottka-Brillout Anna Bettina , Manzo Massimiliano , Trüb Marta , Leonards Katharina , Herzig Petra , Jermann Philipp , Hayoz Stefanie , Natoli Marina , Prince Spasenija Savic , Tochtermann Giulia , Pless Miklos , Bettini Adrienne , Früh Martin , Mauti Laetitia , Britschgi Christian , Peters Solange , Mark Michael , Ochsenbein Adrian , Janthur Wolf-Dieter , Waibel Christine , Mach Nicolas , Froesch Patrizia , Buess Martin , Bohanes Pierre , Gonzalez Michel , Alborelli Ilaria , Rothschild Sacha , Koelzer Viktor , Zippelius Alfred 
Published: April 2024

Abstract
Neoadjuvant chemoimmunotherapy offers promise to improve outcomes for patients with resectable non-small cell lung cancer (NSCLC). Yet, not all patients derive treatment benefits and reliable biomarkers of response are still lacking. We here assess the long-term clinical outcome of neoadjuvant chemotherapy and perioperative anti-PD-L1 inhibition in resectable stage IIIA NSCLC in the SAKK 16/14 trial and provide a comprehensive characterization of anti-tumor immune responses for biomarker-based treatment personalization. At a median follow-up of 5.3 years, the median event-free survival (EFS) was 4.0 years while median overall survival was not reached. Computer-aided spatial image analysis emphasized the importance of CD8+ T cell positioning in tumors, and larger tertiary lymphoid structures in pre-treatment biopsies correlated with improved EFS. Genomic techniques revealed the association of intratumoral TCR diversity with response. Finally, circulating proliferating CD39+ PD-1+ CD8+ T cells and elevated levels of CCL15 post-treatment were seen in patients with sustained therapeutic benefit.
Two opposing yet complementary ocular dominance plasticity: thalamus strengthens the weak channel while higher cortex listens to the strong signal
Authors: Zhang Peng , Qian Yazhu , Sun Zhouyuan , Wang Yizhi , Gao Yige , Qian Chencan , Zhou Jiawei 
Published: April 2024

Abstract
Monocular patching improves the non-patched eye in clinical practice, while recent laboratory evidence suggests that short-term patching strengthens the patched eye instead. The neural mechanisms underlying these opposite effects remain elusive. Using high-resolution 7T fMRI, we found two rapid and opposing mechanisms in the visual thalamus and higher visual cortex in response to monocular contrast deprivation (MCD) in human adults. Short-term MCD shifted ocular dominance of BOLD responses in favor of the deprived eye (DE) in the lateral geniculate nucleus and the ventrolateral pulvinar of the thalamus. Perceptually, DE became more sensitive in contrast detection and more dominant in binocular combination. In higher visual cortices, MCD shifted ocular dominance towards the nondeprived eye (NDE) in BOLD responses and their connectivity from V1. The 3-D shape perception was more accurate from shading cues presented to NDE compared to DE following deprivation. These findings demonstrate that a homeostatic gain modulation mechanism in the thalamus concurrently operates with a rapid Hebbian-like mechanism in the higher cortex to adaptively adjust interocular balance to abnormal binocular input.
Nomogram for predicting postoperative pulmonary infection in elderly patients undergoing major orthopedic surgery
Authors: Liu Yuhan , Yang Tingjun , Fan Yunping , Gan Haibin , Li Xiaohua , Luo Yanrong , Pang Qianyun , Yang Xuping 
Published: April 2024

Abstract

The incidence of postoperative pulmonary infection (PPI) in major orthopedic surgery in the elderly is high, and have a significant impact on perioperative morbidity and mortality. This study aims to develop and validate a nomogram for predicting PPI in elderly patients undergoing major orthopedic surgery. Data included preoperative variables, surgical and anesthesia factors from total of 814 elderly patients undergoing major orthopedic surgery from January 2018 to October 2021 were retrospectively collected. The primary outcome was PPI. The incidence of PPI in this study was 4.2%. Multivariate logistic regression showed that preoperative pulmonary disease (OR:6.018), cognitive impairment (OR:5.285), intraoperative infusion volume ≥ 1200ml (OR:2.693) were independent risk factors for PPI in elderly orthopedic patients. A nomogram was built with 6 risk factors included gender, preoperative pulmonary disease, cognitive impairment and cerebrovascular disease, intraoperative infusion volume, and postoperative analgesia. The area under the curve (AUC) of the nomogram model was 0.800, the slope was 1.000, and the net benefit of the decision curve analysis (DCA) curve was 0.01–0.60. A nomogram for predicting PPI in elderly patients undergoing major orthopedic surgery with 6 variables, can be used to predict PPI of elderly patients undergoing major orthopedic surgery and identify high risk groups.
Genome-wide identifcation of transcripts and miRNAs during somatic embryogenesis in Pinellia ternata
Authors: Xu Rong , Wang Mingxing , Wei Lu , Luo Ming , Miao Yuhuan , Dahui Liu 
Published: April 2024

Abstract

Pinellia ternata(Thunb.) Breit, a well-known Chinese medicinal plants, is mainly produced by clonal propagation. Somatic embryogenesis (SEG) is a major process for plant regeneration, however, the molecular mechanism of SEG in P. ternata is still unclear. In this study, genome-wide mRNAs and small RNAs analysis were performed on the samples from three key stages of P. ternata SEG, including explant, calli and protocorm‐like bodies. The results showed that the increase in the content of auxins, especially I3CA and ICA1, contributed to the regeneration of P. ternata; PtAUX/IAA2, PtSAUR4, PtAEC1, PtAEC2, PtAUT1and PtAUT2 were important regulatory genes for auxins systhesis and signal transduction in P. ternata SEG. For the first time, we employed small RNA sequencing to investigate P. ternata SEG, and we discovered that miR396 were the key miRNAs involved in regulating P. ternata SEG by targeting GRF genes.