bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel approach for identification of tumor‐associated antigens expressed on the surface of tumor cells
Authors: Di Cristina Manlio , Minenkova Olga , Pavoni Emiliano , Beghetto Elisa , Spadoni Andrea , Felici Franco , Gargano Nicola 
Published: December 2006

AbstractTo improve tumor targeting in a subset of patients, where tumor cells do not express the well‐known tumor antigens widely used in immunotherapy, we have developed a novel biotechnological tool. It is useful for tumors of various origins for the identification of tumor‐associated proteins, which are differentially expressed in tumor cells with respect to normal tissue, and exposed on the cell surface. For this purpose, a combination of techniques, such as “suppression subtractive hybridization” and “transmembrane trapping,” was employed. In applying this novel approach to breast cancer, we identified a large panel of cDNA fragments encoding for the well‐known tumor‐associated surface antigens, such as erb‐B2, erbB3 and the urokinase receptor and, more importantly, for several clones overexpressed in breast cancer, whose cDNA fragments match the sequences of hypothetical transmembrane proteins with unknown function. The latter may represent novel tumor‐specific targets. © 2006 Wiley‐Liss, Inc.
Recent trends in breast cancer incidence among 6 Asian groups in the Greater Bay Area of Northern California
Authors: Keegan Theresa H.M. , Gomez Scarlett L. , Clarke Christina A. , Chan Jo Kay , Glaser Sally L. 
Published: December 2006

AbstractAsians and Pacific Islanders are typically aggregated in United States (US) cancer statistics even though the few studies that have considered subgroups separately have found marked differences in cancer incidence. The objective of this study was to evaluate trends in breast cancer incidence rates separately for US Chinese, Japanese, Filipino, Korean, South Asian and Vietnamese women overall and by age at diagnosis, histologic subtype and stage at diagnosis. Age‐adjusted incidence rates and annual percent changes (APC) of new, primary breast cancer diagnosed in the Greater Bay Area Cancer Registry of Northern California (1990–2002) were calculated using SEER*Stat. In women under 50 years of age, annual incidence rates decreased for Japanese (APC = −4.1, p = 0.02) and Filipinas (APC = −1.9, p = 0.11), and increased or fluctuated in other subgroups over the study period. In women 50 years or older, rates of invasive breast cancer increased for most subgroups, except Filipinas (APC = −1.3, p = 0.32), and in Japanese until 1998–2000. Rates of breast cancer in situ increased in most subgroups from 1990 to 2002, as did rates of lobular breast cancer for Chinese (APC = +7.46, p < 0.01) women. In Japanese women, rates of lobular breast cancer were highest in 1995–1997 and decreased thereafter. Our data support the notion that the prevalence of established risk factors influence breast cancer incidence, as breast cancer rates increased for more recently immigrated groups and decreased among more established groups, and may suggest leads into other avenues of research, such as genetic differences, that may explain differences in incidence rates among Asian subgroups. © 2006 Wiley‐Liss, Inc.
Role of glycolysis inhibition and poly(ADP‐ribose) polymerase activation in necrotic‐like cell death caused by ascorbate/menadione‐induced oxidative stress in K562 human chronic myelogenous leukemic cells
Authors: Verrax Julien , Vanbever Stéphanie , Stockis Julie , Taper Henryk , Calderon Pedro Buc 
Published: December 2006

AbstractAmong different features of cancer cells, two of them have retained our interest: their nearly universal glycolytic phenotype and their sensitivity towards an oxidative stress. Therefore, we took advantage of these features to develop an experimental approach by selectively exposing cancer cells to an oxidant insult induced by the combination of menadione (vitamin K3) and ascorbate (vitamin C). Ascorbate enhances the menadione redox cycling, increases the formation of reactive oxygen species and kills K562 cells as shown by more than 65% of LDH leakage after 24 hr of incubation. Since both lactate formation and ATP content are depressed by about 80% following ascorbate/menadione exposure, we suggest that the major intracellular event involved in such a cytotoxicity is related to the impairment of glycolysis. Indeed, NAD+ is rapidly and severely depleted, a fact most probably related to a strong Poly(ADP‐ribose) polymerase (PARP) activation, as shown by the high amount of poly‐ADP‐ribosylated proteins. The addition of N‐acetylcysteine (NAC) restores most of the ATP content and the production of lactate as well. The PARP inhibitor dihydroxyisoquinoline (DiQ) was able to partially restore both parameters as well as cell death induced by ascorbate/menadione. These results suggest that the PARP activation induced by the oxidative stress is a major but not the only intracellular event involved in cell death by ascorbate/menadione. Due to the high energetic dependence of cancer cells on glycolysis, the impairment of such an essential pathway may explain the effectiveness of this combination to kill cancer cells. © 2006 Wiley‐Liss, Inc.
MicroRNA and cancer: Current status and prospective
Authors: Wu Wei , Sun Miao , Zou Gang‐Ming , Chen Jianjun 
Published: December 2006

AbstractGene expression in normal cells is highly regulated by complex gene regulatory networks. Disruption of these networks may lead to cancer. Recent studies have revealed the existence of an abundant class of small nonprotein‐coding regulatory RNAs, known as microRNAs (miRNAs). MiRNAs may regulate diverse biological processes including development, cell proliferation, differentiation and apoptosis, through suppressing the expression of their target genes. Posttranscriptional silencing of target genes by miRNAs occurs either by cleavage of homologous target messenger RNAs (mRNAs), or by inhibition of target protein synthesis. Computational predictions indicate that 1 miRNA may target on hundreds of genes, and suggest that over 50% of human protein‐coding genes might be regulated by miRNAs. MiRNAs are receiving increased attention in cancer genomic research. We are beginning to understand that miRNAs may act as oncogenes and/or tumor suppressor genes within the molecular architecture of gene regulatory networks, thereby contributing to the development of cancer. MiRNAs may provide useful diagnostic and prognostic markers for cancer diagnosis and treatment, as well as serving as potential therapeutic targets or tools. © 2006 Wiley‐Liss, Inc.
Modulation by budesonide of DNA methylation and mRNA expression in mouse lung tumors
Authors: Pereira Michael A. , Tao Lianhui , Liu Yue , Li Long , Steele Vernon E. , Lubet Ronald A. 
Published: December 2006

AbstractBiomarkers are being developed that can aid in the evaluation of cancer therapeutic and chemopreventive drugs. Two suggested biomarkers found in mouse lung tumors are DNA hypomethylation and alterations in mRNA expression of genes, such as 18S RNA, caspase 3, cyclin B2, cyclin E1, iNOS and survivin. Budesonide is very efficacious in preventing lung tumors in mice, so that its ability to modulate biomarkers in lung tumors was determined. Lung tumors were induced by vinyl carbamate in female strain A/J mice. Budesonide (2.0 mg/kg diet) was administered for 2, 7 and 21 days or for 14 days followed by a 7‐days' holding period prior to the killing of the mice at week 27. After 2 days of budesonide treatment, the size of the lung tumors was reduced. Tumor size continued to decrease during the 21 days of treatment. In the tumors, 2 days of treatment resulted in (i) increased methylation of DNA, reversing DNA hypomethylation, (ii) increased expression of 18S RNA and (iii) decreased mRNA expression of caspase 3, cyclin B2, cyclin E1, iNOS and survivin. Termination of budesonide treatment at 7 days prior to killing did not affect the size of the tumors, but did result in increased mRNA expression of the 5 genes, approaching the expression level in tumors from control mice. Hence, budesonide rapidly decreased the size of lung tumors, reversed DNA hypomethylation and modulated mRNA expression of genes; with the molecular alterations requiring continued treatment with the drug for maintenance. © 2006 Wiley‐Liss, Inc.
Small‐sample confidence intervals for multivariate impulse response functions at long horizons
Authors: Pesavento Elena , Rossi Barbara 
Published: December 2006

AbstractExisting methods for constructing confidence bands for multivariate impulse response functions may have poor coverage at long lead times when variables are highly persistent. The goal of this paper is to propose a simple method that is not pointwise and that is robust to the presence of highly persistent processes. We use approximations based on local‐to‐unity asymptotic theory, and allow the horizon to be a fixed fraction of the sample size. We show that our method has better coverage properties at long horizons than existing methods, and may provide different economic conclusions in empirical applications. We also propose a modification of this method which has good coverage properties at both short and long horizons. Copyright © 2006 John Wiley & Sons, Ltd.
Intergenerational mobility and sample selection in short panels
Authors: Francesconi Marco , Nicoletti Cheti 
Published: December 2006

AbstractUsing data from the first 11 waves of the BHPS, this paper measures the extent of the selection bias induced by standard coresidence conditions—bias that is expected to be severe in short panels—on measures of intergenerational mobility in occupational prestige. We try to limit the impact of other selection biases, such as those induced by labour market restrictions that are typically imposed in intergenerational mobility studies, by using different measures of socio‐economic status that account for missing labour market information. We stress four main results. First, there is evidence of an underestimation of the true intergenerational elasticity, the extent of which ranges between 12% and 39%. Second, the proposed methods used to correct for the selection bias seem to be unable to attenuate it, except for the propensity score weighting procedure, which performs well in most circumstances. This result is confirmed both under the assumption of missing‐at‐random data as well as under the assumption of not‐missing‐at‐random data. Third, the two previous sets of results (direction and extent of the bias, and differential abilities to correct for it) are also robust when we account for measurement error. Fourth, restricting the sample to a period shorter than the 11 waves under analysis leads to a severe sample selection bias. In the cases when the analysis is limited to eight waves, this bias ranges from about 40% to 65%. Copyright © 2006 John Wiley & Sons, Ltd.
Identification and estimation of bounds on school performance measures: a nonparametric analysis of a mixture model with verification
Authors: Dominitz Jeff , Sherman Robert P. 
Published: December 2006

AbstractThis paper identifies and nonparametrically estimates sharp bounds on school performance measures based on test scores that may not be valid for all students. A mixture model with verification is developed to handle this problem. This is a mixture model for data that can be partitioned into two sets, one of which (the so‐called verified set) is more likely to be from the distribution of interest than the other. An administrative classification of each student as English proficient or limited English proficient determines these sets. An analysis of performance measures for some California public schools reveals how verification information and plausible monotonicity restrictions can bound the range of disagreement about school performance based on observed scores. Copyright © 2006 John Wiley & Sons, Ltd.
Nuclear receptor interactions in methotrexate induction of human dehydroepiandrosterone sulfotransferase (hSULT2A1)
Authors: Chen Xinrong , Maiti Smarajit , Zhang Jimei , Chen Guangping 
Published: December 2006

AbstractCytosolic sulfotransferases (SULTs) are a major family of phase II drug‐metabolizing enzymes. SULT‐catalyzed sulfonation regulates hormone activities metabolizes drugs detoxifies xenobiotic toxicants bioactivates carcinogens. Human dehydroepiandrosterone sulfotransferase (hSULT2A1 DHEA‐ST) plays a very important role in sulfating endogenous hydroxysteroids and exogenousxenobiotics. Our recent studies have shown that methotrexate can induce hSULT2A1 expression. To investigate the molecular mechanism involved in hSULT2A1 induction we generated the promoter sequence of hSULT2A1 by PCR and constructed a reporter gene vector. Both reporter gene assay and endogenous induction results suggested that human constitutive active receptor (hCAR) mediates the methotrexate induction of hSULT2A1 in both Caco‐2 and Hep G2 cells. Human vitamin D receptor (hVDR) also upregulated hSULT2A1 gene expression while human pregnane X receptor (hPXR) downregulated it. Human pregnane X receptor suppressed hCAR‐mediated methotrexate induction of hSULT2A1 in both Caco‐2 and Hep G2 cells. hVDR competed with hCAR for the hSULT2A1 promoter in Caco‐2 cells. hCAR inhibited hVDR‐mediated vitamin D3 induction of hSULT2A1 but not methotrexate induction of hSULT2A1. These results strongly support the hypothesis that cross‐talk occurs among nuclear receptors in the signal transduction pathway of hSULT2A1 and that interactions among nuclear receptors also depend on ligands (inducers) in the system. © 2006 Wiley Periodicals, Inc. J Biochem Mol Toxicol 20:309–317, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20149
Retracted: Elevated expression of CaMKIIγ during osteoclastogenesis and its functional implications
Authors: Yao Chaohua , Stern Paula H. , Zhang Liang 
Published: December 2006

AbstractCa2+/calmodulin signaling has been recognized recently as a major regulator in osteoclastogenesis. Efforts have ensued to identify the downstream targets of this signaling pathway in the context of regulating osteoclastogenesis. The calcineurin‐NFAT pathway has thus been identified as one such target. In this article, we describe the discovery of another novel downstream target, CaMKIIγ. We also demonstrate that CaMKIIγ is the sole known CaMK expressed in significant amounts in osteoclasts and their precursors. Other known CaMKs such as CaMKIV and CaMKIIα, β, δ, were not detectable, and CaMKI was only expressed at a negligible level. Furthermore, the expression of CaMKIIγ was tightly correlated with the osteoclastogenic process, with a peak level on Day 3 of cell culturing. Osteoclastogenesis is halted by treatment with the CaMKIIγ inhibitor, KN93, independently from apoptosis, with the IC50 for osteoclastogenesis matching that for blocking CaMKIIγ function. Collectively, these data indicate that CaMKIIγ may be a significant regulator of osteoclastogenesis. J. Cell. Biochem. 101: 1038–1045, 2007. © 2006 Wiley‐Liss, Inc.
HiNF‐P is a bifunctional regulator of cell cycle controlled histone H4 gene transcription
Authors: Mitra Partha , Xie Ronglin , Harper J. Wade , Stein Janet L. , Stein Gary S. , van Wijnen Andre J. 
Published: December 2006

AbstractCell cycle progression beyond the G1/S phase transition requires the activation of a transcription complex containing histone nuclear factor P (HiNF‐P) and nuclear protein mapped to ataxia telangiectasia (p220NPAT) in response to cyclin dependent kinase 2 (CDK2)/cyclin E signaling. We show here that the potent co‐activating properties of HiNF‐P/p220NPAT on the histone H4 gene promoter, which are evident in the majority of human cell types, are sporadically neutralized in distinct somatic cell lines. In cells where HiNF‐P and p220NPAT do not activate the H4 gene promoter, HiNF‐P instead represses transcription. Our data suggest that the cell type specific expression of the cyclin‐dependent kinase inhibitory (CKI) protein p57KIP2 inhibits the HiNF‐P dependent activation of the histone H4 promoter. We propose that, analogous to E2F proteins and other cell cycle regulatory proteins, HiNF‐P is a bifunctional transcriptional regulator that can activate or repress cell cycle controlled genes depending on the cellular context. J. Cell. Biochem. 101: 181–191, 2007. © 2006 Wiley‐Liss, Inc.
Tissue‐specific expression of Riken 2810430M08 and its function in T‐cell immune responses
Authors: Wu Tao , Hu ShenJiang , Qiao Xiaoying , Luo Hongyu , Wu Jiangping 
Published: December 2006

AbstractRiken 2810430M08 is a gene with unknown functions. According to in situ hybridization (ISH), it presented a pattern of temporal expression, peaking in the mid‐gestation (embryonic Days e9–e14) stage in most tissues. In the late‐gestation stage and during the adulthood, Riken 2810430M08 was expressed in some tissues with and without a robust proliferation program. Thus, the gene might have important functions that are related and unrelated to proliferation. Its expression was induced after T‐cell receptor stimulation in T cells. However, transgenic mice with actin promoter‐driven expression of Riken 2810430M08 showed normal in vitro and in vivo T cells responses, such as fetal thymus development, adult T cells activation marker expression, lymphokine secretion, proliferation and migration, and delayed type hypersensitivity (DTH). The expression of Riken 2810430M08 during ontogeny has provided useful clues for further investigation. Additional studies are warranted to examine its role in immune and other systems. J. Cell. Biochem. 101: 89–98, 2007. © 2006 Wiley‐Liss, Inc.
Long‐term fluoxetine administration does not result in major changes in bone architecture and strength in growing rats
Authors: Westbroek I. , Waarsing J.H. , van Leeuwen J.P.T.M. , Waldum H. , Reseland J.E. , Weinans H. , Syversen U. , Gustafsson B.I. 
Published: December 2006

AbstractMany studies have indicated that serotonin and its transporter play a role in bone metabolism. In this study we investigated the effect of selective serotonin re‐uptake inhibitor (SSRI), fluoxetine (Prozac®) on bone architecture and quality in growing female rats. We therefore administrated rats with clinically relevant doses of fluoxetine for a period of 6 months. DXA scans were performed during the treatment period in order to follow parameters as body weight, fat percentage and BMD. After 6 months of treatment, femurs were used to analyze bone architecture and bone strength, by means of µCT scans and three‐point bending assays, respectively. We found a slightly diminished bone quality, reflected in a lower bone tissue strength, which was compensated by changes in bone geometry. As leptin and adiponectin could be possible factors in the serotonergic regulation of bone metabolism, we also determined the levels of these factors in plasma samples of all animals. Leptin and adiponectin levels were not different between the control group and fluoxetine‐treated group, indicating that these factors were not involved in the observed changes in bone geometry and quality. J. Cell. Biochem. 101: 360–368, 2007. © 2006 Wiley‐Liss, Inc.
Comparative studies on the pyrolysis of N‐arylideneaminoamides: Kinetic and mechanistic studies
Authors: Al‐Awadi Nouria A. , Ibrahim Yehia A. , Patel Mehul , George Bobby J. , Al‐Etiabi Alya M. 
Published: December 2006

AbstractRates of thermal decomposition of title compounds have been measured using a static reaction system. They undergo a unimolecular first‐order elimination to give arylnitrile and the corresponding substituted amides. The decomposition parallels that of N‐arylidenamino cyclic amide. The relative elimination rates at 600 K were calculated. The kinetic data reveal that the electronic effects of substituents, such as methyl, phenyl, benzyl, and allyl groups, are associated with the opposing directions in which the lone pair of electrons on the nitrogen atom of the arylidene moiety is being delocalized. © 2006 Wiley Periodicals, Inc. Int J Chem Kinet 39: 59–66, 2007
Thermal dehydration kinetics of a rare earth hydroxide, Gd(OH)3
Authors: Chang Chengkang , Mao Dali 
Published: December 2006

AbstractThis paper reports the synthesis, characterization, and dehydration kinetics of a rare earth hydroxide, Gd(OH)3. Uniform rod‐like Gd(OH)3 powder was prepared by a colloidal hydrothermal method. The powder thus obtained dehydrated into its oxide form in a two‐step process, where crystalline GdOOH was obtained as the intermediate phase. Crystal structure study revealed a monoclinic structure for GdOOH, with space group P2/1m and lattice parameters a = 6.0633, b = 3.7107, c = 4.3266, and β = 108.669. The first‐step dehydration follows the F2 mechanism, while the second step follows the F1 model, indicating that both the steps are controlled by nucleation/growth mechanism. The activation energy Ea and frequency factor A are 231±12 kJ/mol and 2.08 × 1018 s−1 for the first step and 496 ± 32 kJ/mol and 7.88 × 1033 s−1 for the second step, respectively. Such high activation energy calculated from the experimental data can be ascribed to the high bonding energy of GdO bond, and the difference in activation energy for the two steps is due to the change in the bond length of hexagonal Gd(OH)3 and monoclinic GdOOH. © 2006 Wiley Periodicals, Inc. Int J Chem Kinet 39: 75–81, 2007
Quantitative analysis of messenger RNA expression of matrix metalloproteinases (MMP‐2 and MMP‐9), tissue inhibitor‐2 of matrix metalloproteinases (TIMP‐2), and steroidogenic enzymes in bovine placentomes during gestation and postpartum
Authors: Takagi M. , Yamamoto D. , Ohtani M. , Miyamoto A. 
Published: December 2006

AbstractThe relationship between the mRNA expression of proteolytic and steroidogenic enzymes in bovine placentomes was examined. Caruncle and cotyledon tissues were collected every 6 hr after spontaneous parturition until the fetal membranes were released. Based on the time of fetal membrane release after parturition, the specimens were classified as follows: (1) the early group, in which the fetal membranes were released within 6 hr after parturition; and (2) the late group, in which the fetal membranes were released 6–12 hr after parturition. The placentomes from a slaughterhouse were additionally collected as samples for the examination of enzymes during the gestation period. The mRNA expression of steroidogenic enzymes in the cotyledon was observed to be higher than that in caruncle tissues; however, the mRNA expression patterns of P450scc and StAR tended to be similar in both placental tissues. On the other hand, although the expression levels of TIMP‐2 mRNA in both caruncle and cotyledon tissues were similar, during gestation and postpartum the expression levels of MMP‐2 and MMP‐9 mRNA were approximately 10 times higher in caruncle than in cotyledon tissue. Marked contrasting changes in mRNA expression patterns between pre‐ and postpartum periods were observed for MMP‐2 and MMP‐9 in caruncle tissues and for MMP‐9 and TIMP‐2 in cotyledon tissues. The present study provides the first evidence that MMP‐2, MMP‐9, and TIMP‐2 mRNAs are expressed in bovine placentomes during the gestational and postpartum periods and suggests that these enzymes, in conjunction with steriodogenic enzymes, mediate fetal membrane detachment after parturition. Mol. Reprod. Dev. 74: 801–807, 2007. © 2006 Wiley‐Liss, Inc.