bluuebunny/crossref_metadata_2025_split huggingface dataset:
Europium Tetracycline as a Luminescent Probe for Nucleoside Phosphates and Its Application to the Determination of Kinase Activity
Authors: Schäferling Michael , Wolfbeis Otto S. 
Published: February 2007

AbstractThe determination of enzyme activities and the screening of enzyme regulators is a major task in clinical chemistry and drug development. A broad variety of enzymatic reactions is associated with the consumption of adenosine triphosphate (ATP), including, in particular, phosphorylation reactions catalyzed by kinases, formation of adenosine cyclic monophosphate (cAMP) by adenylate cyclases, and ATP decomposition by ATPase. We have studied the effect of a series of adenosine (ATP, ADP, AMP, cAMP) and guanosine (GTP, GDP) phosphoric esters, and of pyrophosphate (PP) on the fluorescence emission of the europium tetracycline (EuTC) complex. We found that these compounds have strongly different quenching effects on the luminescence emission of EuTC. The triphosphates ATP and GTP behave as strong quenchers in reducing the fluorescence intensity of EuTC to 25 % of its initial value by formation of a ternary 1:1:1 complex. All other phosphate esters showed a weak quenching effect only. The applicability of this fluorescent probe to the determination of the activity of phosphorylation enzymes is demonstrated by means of creatine kinase as a model for non‐membrane‐bound kinases. In contrast to other methods, this approach does not require the use of radioactively labeled ATP substrates, additional enzymes, or of rather complex immunoassays.
Highly Efficient Cyclization of o‐Iodobenzoates with Aldehydes Catalyzed by Cobalt Bidentate Phosphine Complexes: A Novel Entry to Chiral Phthalides
Authors: Chang Hong‐Tai , Jeganmohan Masilamani , Cheng Chien‐Hong 
Published: February 2007

AbstractMethyl 2‐iodobenzoates 1 a–c undergo cyclization reactions with various aromatic aldehydes 2 a–m (RC6H4CHO: R=H 2 a, 4‐CH3 2 b, 4‐tBu 2 c, 4‐OMe 2 d, 3‐OMe 2 e, 4‐Cl 2 f, 4‐CF3 2 g, 4‐CN 2 h, 4‐Ph 2 i; benzo[d][1,3]dioxole‐5‐carbaldehyde (2 j), 1‐napthaldehyde (2 k), benzofuran‐2‐carbaldehyde (2 l), and isonicotinaldehyde (2 m)) in the presence of [CoI2(dppe)] (dppe=1,2‐bis(diphenylphosphino)ethane) and Zn powder in dry THF at 75 °C for 24 h to give the corresponding phthalide derivatives 3 a–m and 3 q–t in good to excellent yields. Under similar reaction conditions, less reactive aliphatic aldehydes, heptanal (2 n), butyraldehyde (2 o), and 2‐phenylacetaldehyde (2 p) also underwent cyclization reactions with 1 a to provide 3 n–p, respectively, in fair to good yields. The catalytic reaction can be further extended to cinnamyl aldehyde (2 q) with 1 a to give the corresponding phthalide derivative 3 u. This synthetic method is compatible with a variety of functional groups on the aryl ring of 2. The high efficiency of the cobalt catalyst containing a dppe (dppe=1,2‐bis(diphenylphosphino)ethane) ligand encouraged us to investigate the asymmetric version of the present catalytic reaction by employing bidentate chiral ligands. Thus, aromatic aldehydes 2 a–c, 2 f, and 2 g undergo cyclization with 2‐iodobenzoate (1 a) smoothly in the presence of [CoI2{(S,S)‐dipamp}] ((S,S)‐dipamp=(1S,2S)‐(+)‐bis[2‐methoxyphenyl]phenylphosphino)ethane) and zinc powder in THF at 75 °C for 24 h, giving the corresponding (S)‐phthalides 4 a–e in 81–89 % yields with 70–98 % ee. A possible mechanism for the present catalytic reaction is proposed.
Falsely Elevated Cardiac Troponin I Levels
Authors: Makaryus Amgad N. , Makaryus Mary N. , Hassid Babak 
Published: February 2007

AbstractThe measurement of cardiac troponins (cTn) is of considerable usefulness in the diagnosis of acute coronary syndrome. Abnormal levels of serum cTn are occasionally found in patients who are not suffering a myocardial infarction. This may be observed in several well‐known situations including pulmonary embolism, pericarditis, myocarditis, coronary vasospasm, sepsis, congestive heart failure, supraventricular tachycardia with hemodynamic compromise, re‐nal insufficiency, and prolonged strenuous endurance exercise. Endogenous antibodies such as heterophile antibodies, rheumatoid factor, and other autoantibodies are known to interfere with the immunoassay measurements of many different analytes, including the widely used Abbot AxSYM™ cTnI analyzer. Other sources of circulating antibodies include immunotherapies, vaccinations, or blood transfusions that may interfere with these immunoassays as well. We examine the case of a 48‐year‐old man with a history of hypercholesterolemia and obesity who presented with chest pain and was found to have elevated Tn I levels on two separate occasions. Further work‐up revealed that the Tn I levels were spuriously elevated because the patient's blood revealed a normal cTnI level when mixed with polyethylene glycol to inactivate any antibodies interfering with the cTnI assay.
Long‐term results (up to 18 years) of balloon angioplasty on systemic hypertension in adolescent and adult patients with coarctation of the aorta
Authors: Hassan Walid , Malik Shahid , Akhras Nathem , Amri Mohamed Al , Shoukri Mohamed , Fawzy Mohamed Eid 
Published: February 2007

AbstractBackgroundLittle is known regarding the long‐term follow‐up results of balloon angioplasty (BA) for patients with aortic coarctation (AC) on systemic hypertension.HypothesisTo define the long‐term effect of BA of AC on systemic hypertension in adolescent and adult patients.MethodsFollow‐up data of 53 patients (36 male), mean age 24 ± 9 years, undergoing BA for discrete AC at median interval of 11.8 years (range, 4‐18 years), including cardiac catheterization, magnetic resonance imaging (MRI), and echocardiography. Patients were divided into two groups at 1 year after BA on the basis of absence (group A: 40 patients) or presence (group B: 11 patients) of persistent hypertension and need for medication.ResultsFifty‐one patients had baseline hypertension, BA produced an immediate reduction in peak AC gradient from 66 ± 23 mmHg to 10.8 ± 7 mmHg (p < 0.0001). The immediate systolic blood pressure (SBP) decreased from 165 ± 17 mmHg (95% CI: 159 to 171) to 125 ± 10 mmHg (95% CI: 122 to 131) (p < 0.001) in 40 patients (group A) and from 184 ± 19 mmHg (95% CI: 169 to 198) to 142 ± 22 mmHg (95% CI: 124 to 156) (p < 0.001) in 11 patients (group B). At 1‐year follow‐up, SBP decreased further to 115 ± 10 in group A (95% CI 111‐119) and 134 ± 19 in group B (95% CI 122‐142) (p < 0.001). The blood pressure had normalized without medication in group A (165 ± 17 to 115 ± 10 mmHg).ConclusionLong‐term results of BA for discrete AC are excellent and should be considered as the first option for treatment of this disease. No paradoxical hypertension occurred post angioplasty, and normalization of blood pressure without medication occurred in 78% of the patients after BA. Copyright © 2007 Wiley Periodicals, Inc.
Transcoronary concentration gradient of sCD40L and hsCRP in patients with coronary heart disease
Authors: Wang Ying , Li Li , Tan Hong‐Wei , Yu Guang‐Sheng , Ma Zhi‐Yong , Zhao Yu Xia , Zhang Yun 
Published: February 2007

AbstractBackgroundRecent studies indicated that local inflammation played a pivotal role in the pathogenesis of coronary heart disease. Soluble CD40 ligand (sCD40L) and hsC‐ reactive protein (hsCRP) are important inflammatory mediators. However, whether they can reflect local coronary inflammation is unclear.HypothesisWe hypothesized that transcoronary concentration gradient of sCD40L could reflect local inflammation in patients with coronary heart disease (CHD) more reliably.MethodsForty subjects were divided into unstable angina pectoris (UAP) group (n = 20), stable angina pectoris (SAP) group (n = 10), and controls (n = 10). Blood samples were collected from the coronary sinus (CS), aortic root (AO), and femoral vein (FV). The coronary circulation was expressed as CS‐AO difference, while system circulation was expressed as FV‐AO difference. sCD40L and hs‐CRP were measured.ResultsComplex lesions were more frequent in the UAP group than in the SAP group (85% vs. 40%, p < 0.05). CS‐AO differences of sCD40L were much greater in the UAP group than in the SAP or control groups, and were greatly higher than FV‐AO difference in UAP group (465.49 ± 247.85 pg/mL vs. −14.94 ± 83.41 pg/mL; 465.49 ± 247.85 pg/mL vs. −7.66 ± 78.54 pg/mL; 465.49 ± 247.85 pg/mL vs. −7.99 ± 141.34 pg/mL, all p < 0.001). CS‐AO differences of sCD40L were higher in patients with complex lesions than with smooth lesions (657.86 ± 384.76 pg/mL vs. 317.62 ± 409.98 pg/mL, p < 0.01). There were no significant differences of CS‐AO in hs‐CRP among the three groups.ConclusionsIn patients with CHD, the transcoronary concentration gradient of sCD40L is more sensitive than hsCRP, and sCD40L possibly a better marker of local inflammtion and plaque instability. Copyright © 2007 Wiley Periodicals, Inc.
Localization of EB1, IFT polypeptides, and kinesin‐2 in Chlamydomonas flagellar axonemes via immunogold scanning electron microscopy
Authors: Sloboda Roger D. , Howard Louisa 
Published: February 2007

AbstractIntraflagellar transport (IFT) refers to the bi‐directional movement of particles and associated cargo along the axonemes of eukaryotic flagella and cilia. To provide a new perspective on the morphology of IFT particles, their association with the axoneme, and their composition, we have used immunogold localization coupled to detection via scanning electron microscopy. Here we co‐localize in the Chlamydomonas flagellar axoneme polypeptides labeled with specific antibodies. Chlamydomonas EB1 localizes to the distal tip of the axoneme, as expected from previous immunofluorescent data (Pedersen et al. Curr Biol2003;13(22):1969–1974), thus demonstrating the utility of this approach. Using antibodies to IFT‐related polypeptides, particles can be identified associated with the axoneme that fall into one of two classes: The first class is composed of IFT particles labeled with polyclonal antibodies to kinesin‐2 and monoclonal antibodies to either IFT139 (an IFT complex A polypeptide) or IFT172 (a complex B polypeptide). The second class is comprised of particles that label with antibodies to IFT139 alone; thus, discrete particles are present associated with the axoneme that are composed only of complex A polypeptides. When IFT particles were purified by sucrose gradient ultracentrifugation, they appeared as more or less spherical aggregates of varying dimensions labeled with antibodies to IFT139 and to the motor protein kinesin‐2. By contrast, isolated IFT particles that were labeled with IFT172 antibodies were not labeled with kinesin‐2 antibodies. The data are discussed in terms of the total polypeptide composition of an IFT particle and the interaction of the particles with the motors that power IFT. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.
The deaf mouse mutant whirler suggests a role for whirlin in actin filament dynamics and stereocilia development
Authors: Mogensen Mette M. , Rzadzinska Agnieszka , Steel Karen P. 
Published: February 2007

AbstractStereocilia, finger‐like projections forming the hair bundle on the apical surface of sensory hair cells in the cochlea, are responsible for mechanosensation and ultimately the perception of sound. The actin cytoskeleton of the stereocilia contains hundreds of tightly cross‐linked parallel actin filaments in a paracrystalline array and it is vital for their function. Although several genes have been identified and associated with stereocilia development, the molecular mechanisms responsible for stereocilia growth, maintenance and organisation of the hair bundle have not been fully resolved. Here we provide further characterisation of the stereocilia of the whirler mouse mutant. We found that a lack of whirlin protein in whirler mutants results in short stereocilia with larger diameters without a corresponding increase in the number of actin filaments in inner hair cells. However, a decrease in the actin filament packing density was evident in the whirler mutant. The electron‐density at the tip of each stereocilium was markedly patchy and irregular in the whirler mutants compared with a uniform band in controls. The outer hair cell stereocilia of the whirler homozygote also showed an increase in diameter and variable heights within bundles. The number of outer hair cell stereocilia was significantly reduced and the centre‐to‐centre spacing between the stereocilia was greater than in the wildtype. Our findings suggest that whirlin plays an important role in actin filament packing and dynamics during postnatal stereocilium elongation. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.
Honokiol, a natural plant product, inhibits the bone metastatic growth of human prostate cancer cells
Authors: Shigemura Katsumi , Arbiser Jack L. , Sun Shi‐Yong , Zayzafoon Majd , Johnstone Peter A.S. , Fujisawa Masato , Gotoh Akinobu , Weksler Babette , Zhau Haiyen E. , Chung Leland W.K. 
Published: February 2007

AbstractBACKGROUND.Honokiol, a soluble nontoxic natural product derived from Magnolia spp., has been shown to induce apoptosis in malignant cells. The effect of honokiol and the combined therapy with docetaxel on prostate cancer (PCa) growth and bone metastasis was investigated in experimental models.METHODS.The in vitro proapoptotic effects of honokiol on human androgen‐dependent and ‐independent PCa, bone marrow, bone marrow‐derived endothelial, and prostate stroma cells were investigated. Honokiol‐induced activation of caspases was evaluated by Western blot and FACS analysis. To confirm the cytotoxicity of honokiol, mice bone was inoculated in vivo with androgen‐independent PCa, C4‐2 cells and the effects of honokiol and/or docetaxel on PCa growth in bone were evaluated. Daily honokiol (100 mg/kg) and/or weekly docetaxel (5 mg/kg) were injected intraperitoneally for 6 weeks. PCa growth in mouse bone was evaluated by radiography, serum prostate‐specific antigen (PSA) and tissue immunohistochemistry.RESULTS.Honokiol induced apoptosis in all cell lines tested. In PCa cells honokiol induced apoptosis via the activation of caspases 3, 8, and 9 and the cleavage of poly‐adenosine diphosphate ribose polymerase in a dose‐ and time‐dependent manner. Honokiol was shown to inhibit the growth and depress serum PSA in mice harboring C4‐2 xenografts in the skeleton and the combination with docetaxel showed additive effects that inhibited further growth without evidence of systemic toxicity. Immunohistochemical staining confirmed honokiol exhibited growth‐inhibitory, apoptotic, and antiangiogenic effects on PCa xenografts.CONCLUSIONS.The combination of honokiol and low‐dose docetaxel may be used to improve patient outcome in androgen‐independent prostate cancer with bone metastasis. Cancer 2007. © 2007 American Cancer Society.
Influence of age on survival in childhood spitzoid melanomas
Authors: Pol‐Rodriquez Marlyanne , Lee Shing , Silvers David N. , Celebi Julide Tok 
Published: February 2007

AbstractBACKGROUND.Melanoma occurring during childhood and adolescence is rare. Although a few limited studies suggest that the prognosis of childhood melanomas is similar to those in adults, and is dependent on the initial stage of the tumor, there is controversy with respect to the biologic behavior of childhood melanomas. Spitzoid melanoma is a subtype of melanoma with distinct clinical and histopathologic features. The prognosis of spitzoid melanoma in children, despite metastasis, has been suggested to be better than that observed in adults; however, this assertion remains controversial. Whereas a number of spitzoid melanomas with regional lymph node metastasis with no further progression have been reported, cases leading to widespread metastasis and fatal outcomes are also well documented.METHODS.A retrospective review of the literature was conducted between 1949 and 2006. A total of 82 cases of spitzoid melanoma with regional and/or widespread metastasis that occurred in children, 17 years of age and under, were selected for the analysis.RESULTS.The 5‐year survival rate in children diagnosed with metastatic spitzoid melanomas between 0 and 10 years of age was 88% compared with 49% in those between 11 and 17 years of age.CONCLUSIONS.The findings support the notion that younger age (≤10) may be associated with longer survival in children with metastatic spitzoid melanomas. Cancer 2007. © 2007 American Cancer Society.
Effect of laser cleaning on granite color
Authors: Grossi Carlota M. , Alonso Franeisco Javier , Esbert Rosa M. , Rojo Araceli 
Published: February 2007

AbstractThis article presents the effect of laser radiation on the color of ornamental granites used for external cladding. The laboratory experimentation was undertaken on a widely used coarse‐grain granite commercially known as Rosa Porriño. The irradiation was carried out with a Q‐switched Nd:YAG laser at 1064 nm and several energy densities (fluences) on polished surfaces, dry and wet, uncoated, and artificially coated‐simulating a black crust. Laser effects on the granite surface were determined by color measurements with a colorimeter. These measurements made possible to determine probable damage due to laser radiation and the diverse response of different minerals. The analysis of the data also suggests potential causes for the color change and applicability limits of the technique. The a*‐parameter, or red–green component, is the most affected, leading to a change in hab (hue) and was interpreted as a result in variations in the Fe compounds, which strongly condition stone color. No significant changes in L* (luminosity or lightness) or Cab* (chroma) may indicate no relevant alterations in the surface polish. When using laser irradiation to remove black layers on granite surfaces, variations of L* can be indicative of the cleaning effectiveness. This research results may be useful to select laser parameters when managing ornamental granite cleaning operations. However, they also suggest the need of further experimentation in specific techniques of analysis as well as different laser wavelengths. © 2007 Wiley Periodicals, Inc. Col Res Appl, 32, 152 – 159, 2007
Request for existing experimental datasets on color differences
Authors: Melgosa Manuel 
Published: February 2007

AbstractThe Technical Committee 1‐55 of the International Commission on Illumination on “Uniform color space for industrial color difference evaluation” is requesting the submission of datasets for use in developing a new approximately uniform color space for industrial use. The data should be submitted to the TC Chair, Dr. Manuel Melgosa at the University of Granada. © 2007 Wiley Periodicals, Inc. Col Res Appl, 32, 159, 2007
Romero's color invariant in a new light
Authors: Ladson Jack 
Published: February 2007

AbstractRomero et al. [Color Res Appl 2006;31:391–398] have found that modeling sensors as equal‐spread Gaussians in wavelength results in improved illuminant invariance of the Marchant–Onyango daylight expression. I offer some thoughts toward an explanation based on a similar theory for blackbody and related spectra. © 2007 Wiley Periodicals, Inc. Col Res Appl, 32, 160, 2007
Imaginary Vibrational Modes in Polycyclic Aromatic Hydrocarbons: A Challenging Test for the Hardness Profiles
Authors: Torrent‐Sucarrat Miquel , Geerlings Paul , Luis Josep M. 
Published: February 2007

AbstractIn a very recent article (J. Am. Chem. Soc. 2006, 128, 9342–9343) Moran et al. found that electron‐correlated methodologies using popular Pople basis sets lead to spurious nonplanar polycyclic aromatic hydrocarbon (PAH) equilibrium structures. Furthermore, some of the present authors have shown that the hardness profiles along a reaction path can be a useful tool to find spurious stationary points in the potential energy surface. Herein, we test the performance of the hardness profiles to detect shortcomings in energy profiles for the challenging case of nonplanar PAHs. The results obtained show that in 41 of the 42 imaginary vibrational modes studied, the hardness profiles indicate the wrong number and type of the potential energy surface stationary points.
Frequency of BK virus nephropathy in graft dysfunction biopsies
Authors: Çakalagaoglu Fulya , Erbarut Ipek , Tuglular Serhan 
Published: February 2007

AbstractBackgroundBK virus nephropathy is the most common type of viral disease to occur following renal transplantation. Although its frequency differs according to transplantation center, the rate is between 1.5% and 20%. Diagnosis can be established histologically by renal allograft biopsy. The aim of this study was to determine the frequency of BK virus nephropathy in patients who had had a renal biopsy for graft dysfunction at our center.MethodsBiopsies received in the last 3 years from patients with graft dysfunction were used in the study. We have found the BK virus in biopsies of 5 of 41 patients (11%). All these patients had been treated with immunosuppressive drugs following transplantation surgery. Biopsies were performed because of disturbance of renal function. Only 1 patient had had a urinary examination. Morphological diagnosis of BK virus nephropathy was followed by serological examination.ResultsThe most frequent morphological changes with BK virus infection in our samples were basophilia, nuclear inclusions, and nuclear enlargement on tubular epithelium. Decoy cells were not found in the biopsy from the only patient who had had a urinary examination. Treatment of the patients with BK virus nephropathy with immunosuppressive drugs has been reduced, and antiviral treatment has been started.ConclusionBK virus nephropathy should be kept in mind in the differential diagnosis of transplant rejection and drug toxicity.
Cytologic negativity in the diagnosis of secondary pulmonary neoplasms
Authors: Broghamer Walter L. , Richardson Mark E. , Faurest Sharon , Parker James E. 
Published: February 2007

AbstractThis retrospective study consisted of 55patients with histologically confirmed metastatic cancer to the lung. Cytologic specimens were collected within 45 days before death. These included bronchial secretions, pleural effusions, and fine needle aspirations obtained randomly, either singly or in various combinations. The cytodiagnosis of cancer was made in 58% (32/55) of the patients. Originally, 34% (11/32) of these had been false‐negative cases. Morphologic features associated with cytologic negativity in 23 patients included apparent failure to invade the respiratory air compartment in 57% (13/23) of the cases; when applicable, failure to induce a pleural effusion with neoplastic invasion of pleural tissues in 75% (6/8); peripheral distribution of the metastatic nodules to the outer third of the pulmonary parenchyma in 26% (6/23); and tumor size of less than 1.5 cm in 57% (13/23).
Improved antigen detection in ethanol‐fixed cytologic specimens. A modified avidin‐biotin‐peroxidase complex (ABC) method
Authors: Gupta Prabodh K. , Myers Joseph D. , Baylin Stephen B. , Mulshine James L. , Cuttitta Frank , Gazdar Adi F. 
Published: February 2007

AbstractA new amplification technique is described for the detection of small amounts of antigen in ethanol‐fixed cytologic specimens and formalin‐fixed tissues. By adding a third antibody layer to the already sensitive avidin‐biotin immunoperoxidase system. we have demonstrated a considerable increase in both the quantitative and qualitative aspects of this immunostaining method. In evaluating this technique, we employed a previously defined small cell lung carcinoma cell surface antigen system and monoclonal antibodies.
Mucinous metaplasia in scar endometriosis diagnosis by aspiration cytology
Authors: Leiman Gladwyn , Naylor Graeme 
Published: February 2007

AbstractThe clinicopathologic features of a case of scar endometriosis are presented. The lesion is unusual in that it occurred in a black woman following tubal surgery for ectopic pregnancy. The case appears to be unique in that the endometriotic focus was characterized by the presence of mucinous metaplasia. Diagnosis of this histopathologic curiosity was made by fine needle aspiration cytology.