bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Assumption of The Regulatory Function of Nf2-Amot Complex in Early Mammalian Embryos with A Computational Model
Authors: Sakai Yusuke , Hakura Jun 
Published: April 2024

ABSTRACTThe paper assumed that Nf2-Amot complex regulates the phosphorylation cascade so that each cell in the early mammalian embryo differentiates properlyin silico. To confirm the validity of the assumption, it was necessary to verify whether Nf2-Amot complex has an impact on the resulting differentiation. The living embryo is unsuitable for the confirmation since the early mammalian embryo is too small to observe and too ethically sensitive to invade. In such cases, computational models can be used as experimental subjects for operations that cannot be applied to the living embryo. Previous models on the embryo, however, could not verify the assumption because they had not modeled Nf2-Amot complex, and they seldom modeled the Hippo signaling pathway. Therefore, the paper introduced a model of Nf2-Amot complex to the previous study that had modeled the Hippo signaling pathway. Testing the model under diverse conditions revealed that the existence of Nf2-Amot complex reproduces the ideal cell differentiation observed in the living embryo. In this sense, the validity of the model was confirmed. Furthermore, diverse cell-cell contacts that induce various concentrations of Nf2-Amot complex also resulted in ideal cell differentiation. These results suggested the correctness of the assumptionin silico.
Long-Read Structural and Epigenetic Profiling of a Kidney Tumor-Matched Sample with Nanopore Sequencing and Optical Genome Mapping
Authors: Margalit Sapir , Tulpová Zuzana , Detinis Zur Tahir , Michaeli Yael , Deek Jasline , Nifker Gil , Haldar Rita , Gnatek Yehudit , Omer Dorit , Dekel Benjamin , Baris Feldman Hagit , Grunwald Assaf , Ebenstein Yuval 
Published: April 2024

AbstractCarcinogenesis often involves significant alterations in the cancer genome architecture, marked by large structural and copy number variations (SVs and CNVs) that are difficult to capture with short-read sequencing. Traditionally, cytogenetic techniques are applied to detect such aberrations, but they are limited in resolution and do not cover features smaller than several hundred kilobases. Optical genome mapping and nanopore sequencing are attractive technologies that bridge this resolution gap and offer enhanced performance for cytogenetic applications. These methods profile native, individual DNA molecules, thus capturing epigenetic information. We applied both techniques to characterize a clear cell renal cell carcinoma (ccRCC) tumor’s structural and copy number landscape, highlighting the relative strengths of each method in the context of variant size and average read length. Additionally, we assessed their utility for methylome and hydroxymethylome profiling, emphasizing differences in epigenetic analysis applicability.
Calcium (Ca2+) fluxes at Mitochondria-ER Contact Sites (MERCS) are a new target of senolysis in Therapy-Induced Senescence (TIS)
Authors: Puebla-Huerta Andrea , Huerta Hernán , Quezada-Gutierez Camila , Morgado-Cáceres Pablo , Casanova-Canelo César , Linsambarth Sergio , Díaz-Rivera Osman , López-Domínguez José Alberto , Rodríguez-López Sandra , Bustos Galdo , Silva-Pavez Eduardo , Lovy Alenka , Quiroz Gabriel , González-Seguel Catalina , Salas-Huenuleo Edison , Kogan Marcelo J. , Molgó Jordi , Zakarian Armen , Villalba José M. , Gonzalez-Billault Christian , Ahumada-Castro Ulises , Cárdenas J. César 
Published: April 2024

ABSTRACTThis study investigates the state of calcium (Ca2+) flux and Mitochondria-ER contact sites (MERCS) on Therapy-Induced Senescence (TIS).TIS cells-induced by Doxorubicin and Etoposide increase their MERCS contact surface but exhibit a decreased ER-mitochondria Ca2+flux.TIS cells show decreased levels of IP3R isoforms and a decreased interaction between type 1 IP3R isoform and VDAC1.The ER-mitochondria Ca2+flux is essential to maintain the viability of senescence cells.Inhibition of ER-mitochondria Ca2+flux rise as a new target of senolysisin vitroandin vivo.
TheDrosophilaEcR-Hippo component Taiman promotes epithelial cell fitness by control of the Dally-like glypican and Wg gradient
Authors: Schweibenz Colby K. , Placentra Victoria C. , Moberg Kenneth H. 
Published: April 2024

AbstractRapidly dividing cells can eliminate slow growing neighbors through the apoptotic process of cell competition. This process ensures that only high fitness cells populate embryonic tissues and is proposed to underlie the ability of oncogene-transformed cells to progressively replace normal cells within a tissue. Patches of cells in theDrosophilawing disc overexpressing the oncogenic Taiman (Tai) transcriptional coactivator kill normal neighbors by secreting Spz ligands that trigger pro-apoptotic Toll signaling in receiving cells. However, extracellular signaling mechanisms responsible for elimination of slow growing cells by normal neighbors remain poorly defined. Here we show that slow growing cells with reduced Tai (Tailow) are killed by normal neighbors through a mechanism involving competition for the Wingless (Wg/Wnt) ligand. Elevated Wg signaling significantly rescues elimination of Tailowcells in multiple organs, suggesting that Tai may normally promote Wg activity. Examining distribution of Wg components reveals that Tai promotes extracellular spread of the Wg ligand from source cells across the wing disc, thus ensuring patterned expression of multiple Wg-regulated target genes. Tai controls Wg spread indirectly through the extracellular glypican Dally-like protein (Dlp), which binds Wg and promotes its extracellular diffusion and capture by receptors. Data indicate that Tai likely controls Dlp at two levels: transcription ofdlpmRNA and Dlp intracellular trafficking. Overall, these data indicate that the Tai acts through Dlp to enable Wg transport and signaling, and that cell competition in the Tailowmodel arises due to inequity in the ability of epithelial cells to sequester limiting amounts of the Wg growth factor.
Spatio-temporal requirements of Aurora kinase A in mouse oocytes meiotic spindle building
Authors: Blengini Cecilia S. , Vaskovicova Michaela , Schier Jan , Drutovic David , Schindler Karen 
Published: April 2024

SummaryMeiotic spindles are critical to ensure proper chromosome segregation during gamete formation. Oocytes lack centrosomes and use alternative microtubule nucleation pathways for spindle building. However, how these mechanisms are regulated is still unknown. Aurora kinase A (AURKA) is necessary and sufficient for oocyte meiosis in mouse becauseAurkaKO oocytes arrest in meiosis I [1] and AURKA compensates for loss ofAurkb/Aurkc[2]. AURKA is required early in pro-metaphase I to trigger microtubule organizing center fragmentation, a step necessary to effectively build a bipolar spindle. Moreover, in doubleAurkb/Aurkcknockouts, AURKA localizes to spindles and chromatin to support meiosis. Although these mouse models were useful for foundational studies, we were unable to resolve AURKA spatial and temporal functions. Here we provide high-resolution analyses of AURKA requirements during multiple steps of meiotic spindle building and identify the subcellular populations that carry out these functions. By combining mouse genetics and pharmacological approaches we show that AURKA is specifically required in early spindle building and later for spindle stability, whereas AURKC is specifically required in late pro-metaphase. Through expression of targeted AURKA constructs expressed in triple Aurora kinase knockout oocytes and high-resolution live imaging, we demonstrate that the spindle pole population of AURKA is the predominate pool that controls meiotic spindle building and stability.
Phosphorylation on serine 72 modulates Rab7A palmitoylation and retromer recruitment
Authors: Modica Graziana , Tejeda-Valencia Laura , Sauvageau Etienne , Maes Juliette , Skorobogata Olga , Lefrancois Stephane 
Published: April 2024

AbstractThe small GTPase Rab7A has a key role in regulating membrane trafficking at late endosomes. By interacting with several different effectors, this small GTPase controls late endosome mobility, orchestrates fusion events between late endosomes and lysosomes, and participates in the formation of and regulates the fusion between autophagosomes and lysosomes. Rab7A is also responsible for the spatiotemporal recruitment of retromer, which is required for the endosome-to-TGN retrieval of cargo-receptors such as sortilin and CI-MPR. Recently several post-translational modifications have been shown to modulate Rab7A functions, including palmitoylation, ubiquitination and phosphorylation. Here we show that phosphorylation of Rab7A at serine 72 is important to modulate its interaction with retromer, as the non-phosphorylatable Rab7AS72Amutant is not able to interact with and recruit retromer to late endosomes. We have previously shown that Rab7A palmitoylation is also required for efficient retromer recruitment. We found that palmitoylation of Rab7AS72Ais reduced compared to the wild-type protein, suggesting an interplay between S72 phosphorylation and palmitoylation in regulating the Rab7A/retromer interaction. Finally, we identify NEK7 as the kinase required to phosphorylate Rab7A to promote retromer binding and recruitment.
Microwave frequency transfer over 3000-km fiber based on optical frequency combs and active noise cancellation
Authors: Yu Dongrui , Chen Ziyang , Zhang Yufei , Jin Ziyi , Yu Song , Luo Bin , Guo Hong 
Published: April 2024

Time and frequency transfer plays an important role in the fields of navigation and positioning, remote sensing, and fundamental physics. The performance of long-haul frequency transfer is ultimately limited by accumulated phase and amplitude noise in fiber propagation. In this work we overcome these limitations and demonstrate a frequency transfer system over 3000 km of indoor spooled fibers via repetition-frequency-locked frequency combs, which benefit from the extremely high signal-to-noise ratio. With the help of the digital phase discrimination and compensation method and active power stabilization, the dramatic phase and amplitude variations are sufficiently suppressed, respectively, which results in the residual instability 7.5×10−14 at 1 s and 1.0×10−16 at 10 000 s, without optical-electrical-optical recovery by the regeneration system. Our results mark a breakthrough in building large-scale, high-precision synchronization networks.

Published by the American Physical Society
2024
The drivers of external debt in Ghana
Authors: Mensah Lord , Arku Felix Kwasi 
Published: April 2024

PurposeThis paper aims to examine the factors that contribute to the external debt growth in Ghana.Design/methodology/approachThe study adopts the autoregressive distributed lag (ARDL) model and the error correction model (ECM) to establish the short-run and long-run relationships between the dependent variable (external debt) and the independent variables (debt service, exchange rate, gross domestic product, government expenditure, import and trade openness), using a time series data spanning from 1990 to 2019.FindingsThe results indicate that debt service, GDP, government expenditure and trade openness have a positive and significant relationship with external debt, while import and exchange rates have a negative relationship with external debt in the long run. In the short run, debt service, import, exchange rate and trade openness have a positive and significant relationship with external debt, while GDP has a negative relationship with external debt.Practical implicationsThe study found that variables such as government expenditure, debt service and import contribute significantly to the nation’s external debt stock. These findings suggest that policymakers should focus on prioritising and cutting down expenditure in their quest to curtail the debt menace facing the nation. Since existing debt service has the tendency of influencing debt stock, it is recommended that government should reduce borrowing in order avoid debt trap. Home-grown policies to reduce imports must also be encouraged. As these drivers of external debt are tackled head-on, Ghana can be rightly positioned to record lower levels of public debt and subsequently reap the benefits of economic growth.Originality/valueThe study adds to the public debt literature, specifically addressing the idiosyncratic determinants of external debt within the Ghanaian context.
Effect of augmented reality applications on attitude and behaviours of customers: cognitive and affective perspectives
Authors: Iranmanesh Mohammad , Senali Madugoda Gunaratnege , Foroughi Behzad , Ghobakhloo Morteza , Asadi Shahla , Babaee Tirkolaee Erfan 
Published: April 2024

PurposeUnderstanding how to retain users of augmented reality (AR) shopping apps and to motivate them to purchase is vital to the success of AR apps. This study assessed the chain effect of AR attributes on purchase intention and reuse intention through cognitive and affective factors.Design/methodology/approachThe data were collected from Thai users of the IKEA Place app using an online survey. A link to the survey was posted on Thai furniture groups on social media platforms. The 439 responses were analysed using the partial least squares (PLS) approach.FindingsThe results revealed that all four AR attributes, namely interactivity, vividness, novelty and spatial presence, significantly influence perceived enjoyment, perceived diagnosticity and perceived value. Brand attitude, as a key driver of purchase intention, is influenced by perceived value. Attitude towards the app significantly affects reuse intention and is affected by affective and cognitive factors.Practical implicationsThe findings enable shopping app designers and marketers to successfully promote the brand, retain users and boost sales by effectively incorporating AR.Originality/valueThe study extends the literature on the impacts of AR apps on customer behaviours by including affective factors in addition to cognitive factors to explain why AR attributes influence customer attitudes and behaviours. Furthermore, the study demonstrates the serial causal paths from AR attributes to customer behaviours.
Compensatory consumption of specialty agricultural products from an ELM theory perspective: joint effect of product attributes and social affordances
Authors: Wang Lin , Zhao Meng , Zhang Jiangli , Wang Yufang 
Published: April 2024

PurposeCompensatory consumption focuses on the psychological value of products. Special agricultural products have symbolic and social functions that effectively meet psychological needs and stimulate compensatory consumption behavior. The social commerce context not only enriches consumer experience but also influences consumer purchase decisions. This study constructs a model based on the elaboration likelihood model (ELM) and the stimulus-organism-response (SOR) theory to explore the mechanism of compensatory consumption behavior of special agricultural products in a social commerce context.Design/methodology/approachThis study uses a two-stage method of partial least squares structural equation model (PLS-SEM) and fuzzy set qualitative comparative analysis (fsQCA) to analyze 523 valid samples collected through random sampling. PLS-SEM was used to examine the relationships and effects between the variables; fsQCA was used to conduct a cohort analysis between the variables to further reveal the complexity and diversity of compensatory consumption behaviors.FindingsPLS-SEM indicates that product attributes and social affordances influence consumers’ triggering of compensatory consumption behavior for control and belongingness needs. fsQCA shows that there are three different modes, and the satisfaction of belongingness or control needs is a necessary condition for triggering compensatory consumption behavior.Originality/valueThere is limited research on compensatory consumption behavior specifically focused on special agricultural products. This study explores the influencing factors and mechanisms of compensatory consumption behavior related to special agricultural products. The occurrence of compensatory consumption behavior is not only influenced by product attributes but also by the social commerce environment. In marketing strategies, it is important to not only consider product characteristics but also pay attention to consumers’ social and psychological needs.
“What does well-being mean to me?” Conceptualisations of well-being in Irish primary schooling
Authors: Farrell Emma , Symonds Jennifer , Devine Dympna , Sloan Seaneen , Crean Mags , Cahoon Abbie , Hogan Julie 
Published: April 2024

PurposeThe purpose of this study is to understand the meaning of the term well-being as conceptualised by parents, grandparents, principals and teachers in the Irish primary education system.Design/methodology/approachA hermeneutic phenomenological approach was adopted to understand the nature and meaning of the phenomenon of well-being. Interviews were carried out with 54 principals, teachers, parents and grandparents from a representative sample of primary schools in Ireland. Each participant was asked the same, open, question: “What does well-being mean to you?” Responses were transcribed verbatim and analysed using a combination of the principles of the hermeneutic circle and Braun and Clarke’s framework for thematic analysis.FindingsThree conceptualisations of well-being were identified (1) well-being is about being happy, (2) well-being is about being healthy and safe and (3) well-being is something you “do”.Originality/valueTo the best of our knowledge this paper is the first of its kind to describe how well-being is conceptualised by adults in Irish primary school contexts. In particular it highlights how neoliberal conceptualisations of well-being as a “thing”, a commodity exchanged on assumptions of individualism, moralism and bio-economism, have crept into the education of our youngest citizens.
A qualitative study of transformative learning during internships
Authors: Wang Jiunwen , Chia Ivy , Yap Jerry 
Published: April 2024

PurposeThe purpose of this study is to document the process of transformative learning during students’ internships.Design/methodology/approachA qualitative study was conducted with 13 interviewed students to gain deeper insights into their learning experiences during their internships. Their weekly reflections from their 6 month’s internship experience were also coded for common themes.FindingsThe study found numerous trigger events ranging from task-related challenges to interpersonal challenges to environmental challenges led to mindset shifts in students during their internships. The mindset shifts are enabled by students engaging in the trigger events through asking questions, seeking information and reflecting. Other enablers of these mindset shifts are workplace psychological safety, social support and individual learning orientation. The conclusion drawn is that trigger events and enabling resources such as external support are central to healthy mindset shifts and learning.Practical implicationsThis paper provides important guidance for supporting transformative learning during student internships.Originality/valueThis paper provides important guidance for supporting transformative learning during student internships.
Human factors in remote work: examining cyber hygiene practices
Authors: Karayel Tuğçe , Aktaş Bahadır , Akbıyık Adem 
Published: April 2024

Purpose
The purpose of this paper is to investigate the cyber hygiene practices of remote workers.

Design/methodology/approach
This paper used two instruments: first, the Cyber Hygiene Inventory scale, which measures users’ information and computer security behaviors; second, the Recsem Inventory, developed within this paper’s context, to evaluate the cybersecurity measures adopted by organizations for remote workers. It was conducted on remote workers to examine their information security practices. The instrument was administered to a sample of 442 employees reached via the LinkedIn platform. Analyses were performed with SPSS v26, Python programming language and Seaborn library.

Findings
The findings indicate a significant correlation between the security measures implemented by companies and their employees’ cyber hygiene practices. A sector comparison revealed a significant difference in cyber hygiene levels between public and private sector workers.

Research limitations/implications
This paper aims to provide policymakers with suggestions for enhancing the cyber hygiene of remote workers to facilitate compliance with corporate security protocols.

Originality/value
This paper’s conclusions highlight the importance of companies increasing their cybersecurity investments as remote work becomes more prevalent. This should consider not only corporate-level factors but also employees' information and computer security behaviors.
Moderating effect of dynamic capabilities on the relationship between lean practices and operational performance
Authors: Liu Chao-chao , Wang Miao , Niu Zhanwen , Mo Xun 
Published: April 2024

Purpose
The view that dynamic capabilities theory can help explain how lean organizations improve has been put forward by scholars. However, there is still a lack of research on the matching relationship between the application of lean practice and the internal elements of enterprise organization from the perspective of dynamic capabilities. The purpose of this study is to validate the moderating effect of dynamic capabilities on the relationship between lean practices and operational performance.

Design/methodology/approach
This study used the method of survey and empirical research to collect sample data from 263 enterprises in China. Through literature review, this study put forward the moderating hypotheses around dynamic capabilities, lean practices and operation performance and used the method of regression analysis to validate these hypotheses.

Findings
The results showed that dynamic capabilities have a partially moderating effect on the application of lean practices. Specifically, dynamic capabilities have a significant moderating effect on the relationship between just-in-time, total quality management, total preventive maintenance and operational performance, while dynamic capabilities have no significant moderating effect on the relationship between human resource management and operational performance.

Originality/value
The research conclusion complements and enriches the lean practices literature from the perspective of dynamic capabilities. Existing studies mainly focus on the moderating role of external environmental factors, while there is a lack of empirical research on the role of dynamic capabilities in lean practices literature. The research results will help enterprises further understand the matching relationship between lean practices and dynamic capabilities and then improve the success of lean practices application.