bluuebunny/crossref_metadata_2025_split huggingface dataset:
circRNA_8521 promotes Senecavirus A infection by sponging miRNA-324 to regulate LC3A
Authors: Yang Xiwang , Liu Rui , Du Yunsha , Mei Caiqiu , Zhang Guangneng , Wang Chen , Yang Yijun , Xu Zhiwen , Li Wenting , Liu Xiao 
Published: April 2024

AbstractSenecavirus A (SVA) causes outbreaks of vesicular disease in pigs, which imposes a considerable economic burden on the pork industry. As current SVA prevention measures are ineffective, new strategies for controlling SVA are urgently needed. Circular (circ)RNA is a newly characterized class of widely expressed, endogenous regulatory RNAs, which have been implicated in viral infection; however, whether circRNAs regulate SVA infection remains unknown. To investigate the influence of circRNAs on SVA infection in porcine kidney 15 (PK-15) cells, RNA sequencing technology was used to analyze the circRNA expression profiles of SVA-infected and uninfected PK-15 cells, the interactions between circRNAs, miRNAs, and mRNAs potentially implicated in SVA infection were predicted using bioinformatics tools. The prediction accuracy was verified using quantitative real-time (qRT)-PCR, Western blotting, as well as dual-luciferase reporter and RNA pull-down assays. The results showed that 67 circRNAs were differentially expressed as a result of SVA infection. We found that circ_8521 was significantly upregulated in SVA-infected PK-15 cells and promoted SVA infection. circ_8521 interacted with miR-324. miR-324 bound to LC3A mRNA which inhibited the expression of LC3A. Knockdown of LC3A inhibited SVA infection. However, circ_8521 promoted the expression of LC3A by binding to miR-324, thereby promoting SVA infection. We demonstrated that circ_8521 functioned as an endogenous miR-324 sponge to sequester miR-324, which promoted LC3A expression and ultimately SVA infection.
MIR145 Core Promoter Methylation in Pretreatment Cell-Free DNA: A Liquid Biopsy Tool for Muscle-Invasive Bladder Cancer Treatment Outcome
Authors: Pilala Katerina-Marina , Kotronopoulos Georgios , Levis Panagiotis , Giagkos Georgios-Christos , Stravodimos Konstantinos , Vassilacopoulou Dido , Scorilas Andreas , Avgeris Margaritis 
Published: April 2024

PURPOSE The lack of personalized management of bladder cancer (BlCa) results in patients' lifelong post-treatment monitoring with invasive interventions, underlying the urgent need for tailored and minimally invasive health care services. On the basis of our previous findings on miR-143/145 cluster methylation in bladder tumors, we evaluated its clinical significance in pretreatment cell-free DNA (cfDNA) of patients with BlCa. MATERIALS AND METHODS Methylation analysis was performed in our screening cohort (120 patients with BlCa; 20 age-matched healthy donors) by bisulfite-based pyrosequencing. Tumor recurrence/progression for patients with non–muscle-invasive bladder cancer, and progression and mortality for patients with muscle-invasive bladder cancer (MIBC) were used as clinical end point events in survival analysis. Bootstrap analysis was applied for internal validation of Cox regression models and decision curve analysis for assessment of clinical benefit on disease prognosis. RESULTS Decreased methylation of MIR145 core promoter in pretreatment cfDNA was associated with short-term disease progression (multivariate Cox: hazard ratio [HR], 2.027 [95% CI, 1.157 to 3.551]; P = .010) and poor overall survival (multivariate Cox: HR, 2.098 [95% CI, 1.154 to 3.817]; P = .009) of patients with MIBC after radical cystectomy (RC). Multivariate models incorporating MIR145 promoter methylation in cfDNA with tumor stage clearly ameliorated patients' risk stratification, highlighting superior clinical benefit in MIBC prognostication. CONCLUSION Reduced pretreatment cfDNA methylation of MIR145 core promoter was markedly correlated with increased risk for short-term progression and worse survival of patients with MIBC after RC and adjuvant therapy, supporting modern personalized and minimally invasive prognosis. Methylation profiling of MIR145 core promoter in pretreatment cfDNA could serve as a minimally invasive and independent predictor of MIBC treatment outcome and emerge as a promising marker for blood-based test in BlCa.
Repeat Next-Generation Sequencing Testing on Progression in Men With Metastatic Prostate Cancer Can Identify New Actionable Alterations
Authors: Park Joseph J. , Chu Alec , Li Jinju , Ali Alicia , McKay Rana R. , Hwang Clara , Labriola Matthew K. , Jang Albert , Kilari Deepak , Mo George , Ravindranathan Deepak , Graham Laura S. , Sokolova Alexandra , Tripathi Abhishek , Pilling Amanda , Jindal Tanya , Ravindra Aditya , Cackowski Frank C. , Sweeney Patrick L. , Thapa Bicky , Amery Taylor S. , Heath Elisabeth I. , Garje Rohan , Zakharia Yousef , Koshkin Vadim S. , Bilen Mehmet A. , Schweizer Michael T. , Barata Pedro C. , Dorff Tanya B. , Cieslik Marcin , Alva Ajjai S. , Armstrong Andrew J. 
Published: April 2024

PURPOSE There are limited data available on the real-world patterns of molecular testing in men with advanced prostate cancer. We thus sought to evaluate next-generation sequencing (NGS) testing in the United States, focused on single versus serial NGS testing, the different disease states of testing (hormone-sensitive v castration-resistant, metastatic vs nonmetastatic), tissue versus plasma circulating tumor DNA (ctDNA) assays, and how often actionable data were found on each NGS test. METHODS The Prostate Cancer Precision Medicine Multi-Institutional Collaborative Effort clinical-genomic database was used for this retrospective analysis, including 1,597 patients across 15 institutions. Actionable NGS data were defined as including somatic alterations in homologous recombination repair genes, mismatch repair deficiency, microsatellite instability (MSI-high), or a high tumor mutational burden ≥10 mut/MB. RESULTS Serial NGS testing (two or more NGS tests with specimens collected more than 60 days apart) was performed in 9% (n = 144) of patients with a median of 182 days in between test results. For the second NGS test and beyond, 82.1% (225 of 274) of tests were from ctDNA assays and 76.1% (217 of 285) were collected in the metastatic castration-resistant setting. New actionable data were found on 11.1% (16 of 144) of second NGS tests, with 3.5% (5 of 144) of tests detecting a new BRCA2 alteration or MSI-high. A targeted therapy (poly (ADP-ribose) polymerase inhibitor or immunotherapy) was given after an actionable result on the second NGS test in 31.3% (5 of 16) of patients. CONCLUSION Repeat somatic NGS testing in men with prostate cancer is infrequently performed in practice and can identify new actionable alterations not present with initial testing, suggesting the utility of repeat molecular profiling with tissue or blood of men with metastatic castration-resistant prostate cancer to guide therapy choices.
TERT Promoter Mutations Increase Tumor Aggressiveness by Altering TERT mRNA Splicing in Papillary Thyroid Carcinoma
Authors: Sako Ayaka , Matsuse Michiko , Saenko Vladimir , Tanaka Aya , Otsubo Ryota , Morita Michi , Kuba Sayaka , Nishihara Eijun , Suzuki Keiji , Ogi Tomoo , Kawakami Atsushi , Mitsutake Norisato 
Published: April 2024

Abstract

Context
Telomerase reverse transcriptase promoter (TERT-p) mutations, which upregulate TERT expression, are strongly associated with tumor aggressiveness and worse prognosis in papillary thyroid carcinomas (PTCs). TERT expression is also observed in a proportion of PTCs without TERT-p mutations, but such tumors show less aggressiveness and better prognosis than TERT-p mutation–positive tumors.

Objective
TERT has multiple splicing variants whose relationships with the TERT-p status and clinicopathological characteristics remain poorly understood. We examined the relationship between the TERT-p mutational status, the TERT splicing pattern, and clinicopathological features.

Methods
We investigated the expression of 2 major variants, α deletion (dA) and β deletion (dB), in a series of 207 PTCs operated on between November 2001 and March 2020 in Nagasaki University Hospital and Kuma Hospital.

Results
The TERT-p mutations were found in 33 cases, and among 174 mutation-negative cases, 24 showed TERT expression. All cases were classified into 3 groups: the TERT-p mutation–negative/expression–negative group (mut−/exp−), the TERT-p mutation–negative/expression–positive group (mut−/exp+), and the TERT-p mutation–positive group (mut+/exp+). The +A+B/dB ratio in mut+/exp+ was significantly higher than that in mut−/exp+ PTCs. Analysis with clinicopathological data revealed that +A+B expression was associated with higher PTC aggressiveness, whereas dB expression counteracted this effect. Functional in vitro study demonstrated that dB strongly inhibited cell growth, migration, and clonogenicity, suggesting its tumor-suppressive role.

Conclusion
These results provide evidence that the TERT-p mutations alter the expression of different TERT splice variants, which, in turn, associates with different tumor aggressiveness.
The More You Know: The Impact of Personal Knowledge on Interpersonal Treatment at Work
Authors: Hardin Ashley E. 
Published: April 2024

Employees often receive conflicting advice about sharing personal information in the workplace. They are told to “bring your whole self to work” but also to keep it professional and not share too much personal information with colleagues. Research has been equivocal in its overall guidance about sharing personal information at work: it may be either beneficial or harmful for work relationships. These inconsistencies are likely driven by the types of questions posed. Specifically, existing research has studied the impact of a particular piece of personal information and the specific details of what is learned, not what all of these pieces of personal information amount to. Instead, this paper takes a new vantage point to understand how the amount of personal knowledge (the quantity of information that one person knows across many aspects of a colleague’s personal life) influences positive interpersonal dynamics at work by humanizing the known colleague. Through a full-cycle research approach, I establish causal support experimentally and then replicate support for my hypotheses in the field, demonstrating a positive, persistent effect: having more personal knowledge—regardless of whether that knowledge conveys value incongruence or life-to-work interference—leads to a more humanized perception of the known colleague, resulting in increased responsiveness toward that colleague. These findings resolve an existing puzzle in the literature and also contribute to a more nuanced understanding of work relationships and interpersonal learning at work. Funding: This work was supported by Washington University in St. Louis (faculty research funds) and the University of Michigan (doctoral research funds). Supplemental Material: The online appendix is available at https://doi.org/10.1287/orsc.2021.15606 .
Determinants of coexistence of undernutrition and anemia among under-five children in Rwanda; evidence from 2019/20 demographic health survey: Application of bivariate binary logistic regression model
Authors: Asmare Abebew Aklog , Agmas Yitateku Adugna 
Published: April 2024

Background
Undernutrition and anemia are significant public health issues among under-5 children, with potential long-term consequences for growth, development, and overall health. Thus, this study aims to conduct a bivariate binary logistic regression model by accounting for the possible dependency of childhood undernutrition and anemia.

Methods
The data came from the DHS program’s measurement. A total of 3,206 under-five children were involved in this study. A single composite index measure was calculated for stunting, wasting, and underweight using principal component analysis. A bivariate binary logistic regression model is used to assess the association between undernutrition and anemia given the effect of other predictors.

Results
Among 3,206 under-five children considered in this study, 1482 (46.2%) and 658 (20.5%) children were agonized by anemia and undernutrition, respectively. In bivariate binary logistic regression model; Urban children [AOR = 0.751, 96% CI: 0.573–0.984; AOR = 0.663, 95% CI: 0.456–0.995] and anemic mothers [AOR = 1.160, 95% CI: 1.104–1.218; AOR = 1.663, 95% CI: 1.242–2.225] were significantly associated with both childhood anemia and undernutrition, respectively. Improved water sources [AOR = 0.681, 95% CI: 0.446–0.996], average-sized children [AOR = 0.567, 95% CI: 0.462–0.696], and diarrhea [AOR = 1.134, 95% CI: 1.120–2.792] were significantly associated with childhood anemia. Large-sized children [AOR = 0.882, 95% CI: 0.791–0.853] and those with fever [AOR = 1.152, 95% CI: 1.312–2.981] were significantly associated with under-five children’s undernutrition.

Conclusion
The prevalence of both undernutrition and anemia among under-five-year-old children was high in Rwanda. The following determinants are statistically associated with both childhood undernutrition and anemia: place of residence; source of drinking water; maternal anemia; being a twin; birth size of children; diarrhea; fever; and child age. Anemia and nutritional deficiencies must be treated concurrently under one program, with evidence-based policies aimed at vulnerable populations.
Uncovering the causes and socio-demographic constructs of stillbirths and neonatal deaths in an urban slum of Karachi
Authors: Muhammad Ameer , Rizvee Muhammad Salman Haider , Khan Uzma , Khan Hina , Bachlany Alishan , Baloch Benazir , Shafiq Yasir 
Published: April 2024

Introduction
Neonatal deaths and stillbirths are significant public health concerns in Pakistan, with an estimated stillbirth rate of 43 per 1,000 births and a neonatal mortality rate of 46 deaths per 1,000 live births. Limited access to obstetric care, poor health seeking behaviors and lack of quality healthcare are the leading root causes for stillbirths and neonatal deaths. Rehri Goth, a coastal slum in Karachi, faces even greater challenges due to extreme poverty, and inadequate infrastructure. This study aims to investigate the causes and pathways leading to stillbirths and neonatal deaths in Rehri Goth to develop effective maternal and child health interventions.

Methods
A mixed-method cohort study was nested with the implementation of large maternal, neonatal and child health program, captured all stillbirths and neonatal death during the period of May 2014 till June 2018. The Verbal and Social Autopsy (VASA) tool (WHO 2016) was used to collect primary data from all death events to determine the causes as well as the pathways. Interviews were conducted both retrospectively and prospectively with mothers and caregivers. Two trained physicians reviewed the VASA form and the medical records (if available) and coded the cause of death blinded to each other. Descriptive analysis was used to categorize stillbirth and neonatal mortality data into high- and low-mortality clusters, followed by chi-square tests to explore associations between categories, and concluded with a qualitative analysis.

Results
Out of 421 events captured, complete VASA interviews were conducted for 317 cases. The leading causes of antepartum stillbirths were pregnancy-induced hypertension (22.4%) and maternal infections (13.4%), while obstructed labor was the primary cause of intrapartum stillbirths (38.3%). Neonatal deaths were primarily caused by perinatal asphyxia (36.1%) and preterm birth complications (27.8%). The qualitative analysis on a subset of 40 death events showed that health system (62.5%) and community factors (37.5%) contributing to adverse outcomes, such as delayed referrals, poor triage systems, suboptimal quality of care, and delayed care-seeking behaviors.

Conclusion
The study provides an opportunity to understand the causes of stillbirths and neonatal deaths in one of the impoverished slums of Karachi. The data segregation by clusters as well as triangulation with qualitative analysis highlight the needs of evidence-based strategies for maternal and child health interventions in disadvantaged communities.
Beneficial impacts of physical activity on heart rate variability: A systematic review and meta-analysis
Authors: El-Malahi Ouahiba , Mohajeri Darya , Mincu Raluca , Bäuerle Alexander , Rothenaicher Korbinian , Knuschke Ramtin , Rammos Christos , Rassaf Tienush , Lortz Julia 
Published: April 2024

Background
Cardiovascular diseases (CVD) are the leading causes of morbidity and mortality. Heart rate variability (HRV) represents the modulatory capacity of the autonomous nervous system and influences mortality. By surveying this meta-analysis, we investigated the impact of physical activity on HRV.

Methods
Databases, online journal libraries and clinical trial registries were searched for publications of randomized controlled and non-randomized controlled trials concerning adults with coronary artery disease (CAD)/ischemic heart disease (IHD), congestive heart failure (CHF), peripheral arterial disease (PAD) or after acute coronary syndrome (ACS) joining an intervention group with physical activity or a control group with usual care or no intervention. Extracted time-domain and frequency-domain parameter of HRV were analyzed in a meta-analysis using a random effect model. Subgroup analyses concerning intervention type, study design and type of heart disease and sensitivity analysis were performed.

Results
Significant results were obtained for RR-Interval (p = 0.05) and standard deviation of Normal-to-Normal intervals (SDNN) (p = 0.01) for short-term assessment and for the ratio of low-frequency power (LF) to high-frequency power (HF) (p = 0.05) for 24-hour assessment. Subgroup analyses also resulted significant: root-mean-square difference of successive normal R-R intervals (RMSSD) (p = 0.01), SDNN (p = 0.02) and HF (p < 0.01) concerning CHF.

Conclusion
We were able to demonstrate the positive impact of physical activity on HRV, especially in patients with CHF. Cardiac rehabilitation exercise programs need to be individualized to identify the most beneficial method of training for improving the prognosis of patients with CVD.
Fables of Development: Capitalism and Social Imaginaries in Spain (1950–1967), Ana Fernández-Cebrian (2023)
Authors: Castelló Enric 
Published: April 2024

Review of: Fables of Development: Capitalism and Social Imaginaries in Spain (1950–1967), Ana Fernández-Cebrian (2023)
Liverpool: Liverpool University Press, 210 pp.,
ISBN 978-1-80207-805-3, h/bk, £95
ISBN 978-1-80207-641-7, e-book, £95
Reggaetón: Una Revolución Latina (Reaggeton: a Latin Revolution), Pablito Wilson (2022)
Authors: Jiménez Pau Albà 
Published: April 2024

Review of: Reggaetón: Una Revolución Latina (Reaggeton: a Latin Revolution), Pablito Wilson (2022)
Bilbao: Liburuak
ISBN 9-788-41923-4-018, h/bk, €25.00
P2X7 receptor: A receptor closely linked with sepsis-associated encephalopathy
Authors: Fan Zhao , Wang Kaifang , Zhao Xiaoyong , Sun Xude 
Published: April 2024

Abstract
Sepsis is defined as a dysregulated host response to infection resulting in life-threatening organ dysfunction. Sepsis-associated encephalopathy (SAE) is the main manifestation of sepsis. Inflammation, peroxidation stress injury, and apoptosis are the main factors involved in the pathogenesis of SAE. A growing body of evidence has proved that P2X7 receptor (P2X7R), a cationic channel receptor that is widely distributed in the body, plays a major role in the occurrence and development of inflammatory injury. Therefore, this review mainly describes the activation of P2X7R in sepsis, which leads to the recruitment of inflammatory cells to the cerebral vasculature, the destruction of the blood–brain barrier, the activation of microglial cells in the brain, the apoptosis of brain cells, and other damage processes. This review also illustrates the potential therapeutic value of P2X7R inhibition in SAE.
Injustice, Corruption, and Partisanship in the Eastern Seljuq Lands of the Early Twelfth Century: al-Ghazālī’s Persian Letters to Viziers as a Historical Source
Authors: Tor D. G. 
Published: April 2024

Abstract
This article analyses five historical missives written by the great theologian Abū Ḥāmid al-Ghazālī to three Seljuq viziers, and datable to the years 490/1097 to 504/1110 f. The first four of these letters form a progressive series complaining of governmental corruption in Ṭūs during the Seljuq civil war around the turn of the sixth/twelfth century, and thus provide the sole extant eyewitness testimony from Khurāsān during the last five years of the Seljuq civil war period. They also reveal al-Ghazālī’s vision of just rule far more concretely than do al-Ghazālī’s numerous theoretical writings on the subject. The fifth letter, written after the civil war had ended, explores the problem of intra-Sunni madhhab-based religious partisanship (taʿaṣṣub), and also incidentally reveals important biographical information about al-Ghazālī. All five of these letters constitute unique eyewitness testimony to some of the major political and social problems of the Seljuq period.
Stylistic classification of cuneiform signs using convolutional neural networks
Authors: Yugay Vasiliy , Paliwal Kartik , Cobanoglu Yunus , Sáenz Luis , Gogokhia Ekaterine , Gordin Shai , Jiménez Enrique 
Published: April 2024

Abstract
The classification of cuneiform signs according to stylistic criteria is a difficult task, which often leaves experts in the field disagree. This study introduces a new publicly available dataset of cuneiform signs classified according to style and Convolutional Neural Network (CNN) approaches to differentiate between cuneiform signs of the two main styles of the first millennium bce, Neo-Assyrian and Neo-Babylonian. The CNN model reaches an accuracy of 83 % in style classification. This tool has potential implications for the recognition of individual scribes and the dating of undated cuneiform tablets.
The bat Tonatia bidens (Phyllostomidae) as an insect pest predator in the Brazilian Caatinga
Authors: Barbier Eder , Nobre Carlos Eduardo Beserra , Iannuzzi Luciana , Bernard Enrico 
Published: April 2024

Abstract

Tonatia bidens is listed as ‘Data Deficient’ in the IUCN Red List. Our research, conducted in the Brazilian Caatinga dry forest between 2016 and 2022, aimed to shed light on the diet of this little-known species. Thirty-eight taxa were identified among insect remains beneath T. bidens’ feeding perch. Notably, certain insects such as hawkmoths and cerambycid beetles are agricultural pests with significant negative impacts on crops, including cassava, corn, tomato, papaya, and cashew plantations. These results underscore bats’ crucial role in insect pest consumption, emphasizing the need for further studies to fully understand their diet and ecosystem contributions.
Long non-coding RNA SRA1 suppresses radiotherapy resistance in esophageal squamous cell carcinoma by modulating glycolytic reprogramming
Authors: Chen Yurao , Fan Peng , Chen Zhenhai , Zheng Zemao , He Ming , Zhao Xiang , Chen Ronghuai , Yao Juan , Yang Zhaodong 
Published: April 2024

Abstract
Esophageal squamous cell carcinoma (ESCC), a highly aggressive subtype of esophageal cancer, is characterized by late-stage diagnosis and limited treatment options. Recent advancements in transcriptome sequencing technologies have illuminated the molecular intricacies of ESCC tumors, revealing metabolic reprogramming as a prominent feature. Specifically, the Warburg effect, marked by enhanced glycolysis, has emerged as a hallmark of cancer, offering potential therapeutic targets. In this study, we comprehensively analyzed bulk RNA-seq data from ESCC patients, uncovering elevated SRA1 expression in ESCC development and a poorer prognosis. Silencing of SRA1 led to a modulation of glycolysis-related products and a shift in PKM2 expression. Our findings shed light on the intricate molecular landscape of ESCC, highlighting SRA1 as a potential therapeutic target to disrupt glycolysis-dependent energy production. This metabolic reprogramming may hold the key to innovative treatment strategies for ESCC, ultimately improving patient outcomes.