bluuebunny/crossref_metadata_2025_split huggingface dataset:
Copper binding and conformation of the N‐terminal octarepeats of the prion protein in the presence of DPC micelles as membrane mimetic
Authors: Dong Shou‐Liang , Cadamuro Sergio A. , Fiorino Ferdinando , Bertsch Uwe , Moroder Luis , Renner Christian 
Published: October 2007

AbstractThe prion protein is usually pictured as globular structured C‐terminal domain that is linked to an extended flexible N‐terminal tail. However, in its physiological form, it is a glycoprotein tethered to the cell surface via a C‐terminal GPI anchor. The low solubility of PrP even without GPI anchor and its strong tendency for aggregation has forced most structural investigations to be performed at low pH and mostly with N‐terminally truncated variants. In the present study, we have used a synthetic peptide related to the PrP tetra‐octarepeat region, i.e., the sequence (Pro‐His‐Gly‐Gly‐Gly‐Trp‐Gly‐Gln)4, for NMR structural analysis of its preferred conformation in DPC micelles as membrane mimic. Well‐defined and identical loops are observed between the four octarepeats that are linked by flexible Gly‐Gly‐Gly sequences. Interaction with the micelles is mainly through the tryptophan residues that appear to act as anchors. Copper binding to the peptide in the presence of DPC micelles revealed marked conformational rearrangements although binding to the micelles is preserved. Interestingly, titration experiments point to cooperative effects for the four binding sites. A destabilization of the DPC micelles by the peptide parallels the destabilizing effect of the prion protein on membranes so that the octarepeat region appears to be very membrane‐active. How the physico–chemical properties reported here are linked to the function and significance of the prion protein remains a puzzle as long as the functional mechanism of the prion protein is not precisely elucidated. Nevertheless, our results emphasize the strong influence of the (membrane) environment on the PrP properties. © 2007 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 88: 840–847, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
The PTB domain of tensin: NMR solution structure and phosphoinositides binding studies
Authors: Leone Marilisa , Yu Eric C. , Liddington Robert C. , Pasquale Elena B. , Pellecchia Maurizio 
Published: October 2007

AbstractTensin is a protein confined at those discrete and specialized regions of the plasma membrane, known as focal adhesions. It contains, at the C‐terminus, a phosphotyrosine binding (PTB) domain that can interact with the cytoplasmic tail of β‐integrins and is necessary for localization of the protein to cell‐matrix adhesions. Here, we present the NMR solution structure of the PTB domain of tensin1. Moreover, through NMR binding studies, we demonstrate that the PTB domain of tensin1 is able to interact with phosphatidylinositol 4, 5‐diphosphate (PtIns(4,5)P2) and phosphatidylinositol 4‐phosphate (PtIns(4)P), presenting higher affinity for the diphosphorylated inositide. Chemical shift mapping studies reveal a putative PtIns(4,5)P2 binding region that is distinct from the predicted integrin β‐tail recognition site. Heteronuclear NOE experiments, recorded in absence and presence of PtIns(4,5)P2, indicate that the interaction with lipids decreases the flexibility of loop regions, predicted to be important for integrin binding, and thus, proposes a possible correlation between the two distinct binding events. Therefore, our studies suggest that capture of lipids by the PTB domain of tensin1 may play a role for the protein function at focal adhesions. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 86–92, 2008.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
DartBoards and Clovers as new tools in sustainability planning and control
Authors: Bonacchi Massimiliano , Rinaldi Leonardo 
Published: October 2007

AbstractAmong organizations that recognize that a multidimensional perspective is necessary to integrate stakeholder needs into a long term value creation process, only in a few cases are performance measurement systems able to integrate financial indicators with social and environmental metrics. This paper first discusses the sustainability concept for management accounting purposes. After reviewing the literature addressing performance measurement system issues, the paper offers a view as to how implementation of management accounting for sustainability could progress. We suggest a performance measurement system based on two managerial instruments that organize a set of primary and secondary measures, connected with stakeholder satisfaction, and are able to detect and articulate both win–win and trade‐off situations. Copyright © 2007 John Wiley & Sons, Ltd and ERP Environment.
Paving the way for sustainability communication: evidence from a Swiss study
Authors: Stiller Ylva , Daub Claus‐Heinrich 
Published: October 2007

AbstractThis paper presents and compares the outcomes of an annually repeated project, assessing the corporate reporting practice in the 250 largest Swiss companies, over a time frame of three years (2003–2005). Focusing on the quantitative and qualitative corporate sustainability disclosure, the study attempts a review of the Swiss corporate reporting practice by using a benchmark approach, as an alternative to content analysis, in order to supply best‐practice examples for companies and open the door towards a more dialogical approach. Supported by the findings of Kolk, Gray, Unerman etc. as well as critique aired by Thomson and Bebbington and by Solomon and Darby, the study and its results are discussed and reflected upon in a conceptual and practical context. Copyright © 2007 John Wiley & Sons, Ltd and ERP Environment.
Improvement of residual bilinearization by particle swarm optimization for achieving the second‐order advantage with unfolded partial least‐squares
Authors: Bortolato Santiago A. , Arancibia Juan A. , Escandar Graciela M. , Olivieri Alejandro C. 
Published: October 2007

AbstractThe combination of unfolded partial least‐squares (U‐PLS) with residual bilinearization (RBL) provides a second‐order multivariate calibration method capable of achieving the second‐order advantage. RBL is performed by varying the test sample scores in order to minimize the residues of a combined U‐PLS model for the calibrated components and a principal component model for the potential interferents. The sample scores are then employed to predict the analyte concentration, with regression coefficients taken from the calibration step. When the contribution of multiple potential interferents is severe, particle swarm optimization (PSO) helps in preventing RBL to be trapped by false minima, restoring its predictive ability and making it comparable to the standard parallel factor (PARAFAC) analysis. Both simulated and experimental systems are analyzed in order to show the potentiality of the new technique. Copyright © 2007 John Wiley & Sons, Ltd.
Novel application of flow cytometry: Determination of muscle fiber types and protein levels in whole murine skeletal muscles and heart
Authors: Jackaman Connie , Nowak Kristen J. , Ravenscroft Gianina , Lim Esther M. , Clément Sophie , Laing Nigel G. 
Published: October 2007

AbstractConventional methods for measuring proteins within muscle samples such as immunohistochemistry and western blot analysis can be time consuming, labor intensive and subject to sampling errors. We have developed flow cytometry techniques to detect proteins in whole murine heart and skeletal muscle. Flow cytometry and immunohistochemistry were performed on quadriceps and soleus muscles from male C57BL/6J, BALB/c, CBA and mdx mice. Proteins including actins, myosins, tropomyosin and α‐actinin were detected via single staining flow cytometric analysis. This correlated with immunohistochemistry using the same antibodies. Muscle fiber types could be determined by dual labeled flow cytometry for skeletal muscle actin and different myosins. This showed similar results to immunohistochemistry for I, IIA and IIB myosins. Flow cytometry of heart samples from C57BL/6J and BALB/c mice dual labeled with cardiac and skeletal muscle actin antibodies demonstrated the known increase in skeletal actin protein in BALB/c hearts. The membrane‐associated proteins α‐sarcoglycan and dystrophin could be detected in C57BL/6J mice, but were decreased or absent in mdx mice. With the ability to label whole muscle samples simultaneously with multiple antibodies, flow cytometry may have advantages over conventional methods for certain applications, including assessing the efficacy of potential therapies for muscle diseases. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.
Homogenization of Hamilton‐Jacobi‐Bellman equations with respect to time‐space shifts in a stationary ergodic medium
Authors: Kosygina Elena , Varadhan S. R. S. 
Published: October 2007

AbstractWe consider a family {uϵ(t, x, ω)}, ϵ < 0, of solutions to the equation ∂uϵ/∂t+ ϵΔuϵ/2 +H(t/ϵ,x/ϵ, ∇uϵ, ω) = 0 with the terminal datauϵ(T, x, ω) =U(x). Assuming that the dependence of the HamiltonianH(t, x, p, ω) on time and space is realized through shifts in a stationary ergodic random medium, and thatHis convex inpand satisfies certain growth and regularity conditions, we show the almost sure locally uniform convergence, in time and space, ofuϵ(t, x, ω) as ϵ → 0 to the solutionu(t, x) of a deterministic averaged equation ∂u/∂t+H̄(∇u) = 0,u(T, x) =U(x). The “effective” HamiltonianH̄is given by a variational formula. © 2007 Wiley Periodicals, Inc.
Investigating the performance of a middleware protocol architecture for tele‐measurement
Authors: Berruti Luca , Vignola Stefano , Zappatore Sandro 
Published: October 2007

AbstractThe rapid growth of network infrastructures and the large availability of instrumentation supporting remote control have encouraged the deployment of complex and sophisticated laboratories and the design of software platforms for accessing the resources present there. Although the market offers several solutions to remotely manage equipment, little attention has been paid to the hardware and software architectures devoted to control distance learning experimental environments and to manage laboratories consisting of heterogeneous devices. The paper illustrates the architectural approach adopted within the LABNET project and describes in detail the main software components of the devised platform, which allows to exploit the instrumentationviaa common Web user interface, thus making the system available independent of any specific (commercial) environment or application. Specifically, attention is focused on the LABNET server (LNS), which represents the supervising central unit and, therefore, a very critical element of the system. The paper mainly points out the architecture and protocols at the basis of the LNS and discusses a set of performance tests aimed at proving the effectiveness of the system and comparing it with a well‐known commercial solution. Copyright © 2007 John Wiley & Sons, Ltd.
A neural network system for authenticating remote users in multi‐server architecture
Authors: Lin Iuon‐Chang 
Published: October 2007

AbstractAuthenticating the legitimacy of a remote user is an important issue in modern computer systems. In this paper, a neural network system for authenticating remote users is presented. The benefits of the proposed scheme include that (1) it is suitable for multi‐server environment; (2) it does not maintain a verification table; (3) users can freely choose their password; and (4) it can withstand replay attack, off‐line password guessing attack, and privileged insider attacks. Furthermore, some drawbacks, such as the users who choose the same passwords will have the same identities and unavailability for evicting a user from the system, will also be eliminated. Copyright © 2007 John Wiley & Sons, Ltd.
Pancreatic scintigraphy with 99mTc‐interleukin‐2 at diagnosis of type 1 diabetes and after 1 year of nicotinamide therapy
Authors: Chianelli M. , Parisella M. G. , Visalli N. , Mather S. J. , D'Alessandria C. , Pozzilli P. , Signore A. ,   IMDIAB study group
Published: October 2007

AbstractBackgroundTo evaluate the clinical utility of pancreatic scintigraphy with 99mTc‐interleukin‐2 to identify Type 1 diabetic patients with pancreatic inflammation at diagnosis.Methods99mTc‐interleukin‐2 scintigraphy was performed on 42 newly diagnosed Type 1 diabetic patients, before and after 1 year of treatment with nicotinamide (25 or 50 mg/kg/day) in addition to intensive insulin therapy. Metabolic status was monitored every 3 months for 1 year. Sixteen normal subjects were studied as control.ResultsSignificant pancreatic accumulation of 99mTc‐interleukin‐2 was found in 31% of the patients at the time of diagnosis. Patients positive or negative for pancreatic accumulation of interleukin‐2 scintigraphy did not show any difference in metabolic or immunologic parameters at diagnosis. Positive patients, however, showed higher C‐peptide values at 3 months and lower insulin requirement at 1 year, compared to negative patients (insulin requirement (IR): 0.33 ± 0.11 vs 0.67 ± 0.24 IU/kg/day, positive vs negative patients; p = 0.0001); patients positive to IL2 scintigraphy treated with nicotinamide at 25 mg/kg were the only group showing a significant reduction in IR 1 year after diagnosis (IRt0: 0.53 ± 0.30 vs IRt12: 0.28 ± 0.07 IU/kg/day; p = 0.013). After 1 year, all the positive patients showed a significant decrease in pancreatic uptake of 99mTc‐interleukin‐2 (P/B: 7.87 ± 2.28 at diagnosis vs 5.00 ± 1.23 after 1 year; p < 0.0001 paired t‐test).Conclusion99mTc‐interleukin‐2 scintigraphy at diagnosis of Type 1 diabetes may identify patients with pancreatic inflammation. In such patients, treated with nicotinamide at 25 mg/kg, insulin requirement and pancreatic inflammation after 1 year were significantly reduced suggesting that IL2 scintigraphy may be of potential use for assessing the autoimmune phenomena in endocrine pancreas. Copyright © 2007 John Wiley & Sons, Ltd.
Impact of lowering the criterion for impaired fasting glucose on identification of individuals with insulin resistance. The GISIR database.
Authors: Sesti Giorgio , Andreozzi Francesco , Bonadonna Riccardo C. , De Mattia Giancarlo , Leonetti Frida , Luzi Livio , Marini Maria Adelaide , Natali Andrea , Vettor Roberto , Bonora Enzo ,   on behalf of the Group of Italian Scientists of Insulin Resistance (GISIR)
Published: October 2007

AbstractObjectiveWe assessed the accuracy of the American Diabetes Association (ADA)2003 definition of impaired fasting glucose (IFG) in identifying subjects with low insulin sensitivity, and determined cardiovascular risk factors in ADA2003 IFG subjects.Research Design and MethodsThis study included 930 non‐diabetic Italian Caucasians from the GISIR database in which subjects underwent a hyperinsulinaemic‐euglycaemic clamp performed with a standard technique. Low insulin sensitivity was defined as being in the lower quartile of glucose metabolized during the last hour of the clamp (M). Subjects were stratified in the following groups: normal fasting glucose (NFG) (<100 mg/dL), IFG100 (100–109 mg/dL), ADA1997 IFG110 (110–125 mg/dL), and ADA2003 IFG (100–125 mg/dL).ResultsThe sensitivity of identifying subjects with low insulin sensitivity increased adopting the ADA2003 criterion. After Bonferroni correction for multiple comparisons, both IFG100 and ADA1997 IFG110 showed significantly higher body mass index (BMI), waist, systolic blood pressure (SBP) and diastolic blood pressure (DBP), triglyceride, fasting plasma insulin (FPI) and fasting plasma glucose (FPG), and lower insulin sensitivity as compared with NFG. As compared with IFG100, ADA1997 IFG110 showed significantly higher BMI, waist, SBP, FPI, FPG, and lower insulin sensitivity. ADA2003 IFG group showed significantly higher BMI, waist, SBP and DBP, triglyceride, cholesterol, FPI, and FPG, but lower HDL levels and insulin sensitivity compared with NFG subjects.ConclusionsAlthough neither the ADA2003 nor the ADA1997 definition of IFG appears to be particularly efficacious for the identification of subjects' low insulin sensitivity, lowering the criterion to the ADA2003 glucose threshold increased the sensitivity without affecting the specificity. ADA2003 IFG showed a worse cardiovascular risk profile compared with NFG.Copyright © 2007 John Wiley & Sons, Ltd.
Insulin glargine and receptor‐mediated signalling: clinical implications in treating type 2 diabetes
Authors: Le Roith Derek 
Published: October 2007

AbstractMost patients with type 2 diabetes mellitus will eventually require insulin therapy to achieve or maintain adequate glycaemic control. The introduction of insulin analogues, with pharmacokinetics that more closely mimic endogenous insulin secretion, has made physiologic insulin replacement easier to achieve for many patients. However, there are also concerns regarding alteration of binding affinities for the insulin receptor (IR) or insulin‐like growth factor‐1 receptor (IGF‐1R) may increase the mitogenic potential of some analogues. Therefore, this article will review the relevant preclinical and clinical data to assess the mitogenic potential of insulin glargine, a basal insulin analogue, compared with regular human insulin (RHI).Searches of the PubMed database were performed using terms that included ‘IR,’ ‘insulin‐like growth factor‐1,’ ‘IGF‐1R,’ ‘type 2 diabetes mellitus,’ and ‘insulin glargine.’ Original articles and reviews of published literature were retrieved and reviewed.Although one study reported increased binding affinity of insulin glargine for the IGF‐1R and increased mitogenic potential in cells with excess IGF‐1Rs (Saos/B10 osteosarcoma cells), most in vitro binding‐affinity and cell‐culture studies have demonstrated behaviour of insulin glargine comparable to that of RHI for both IR and IGF‐1R binding, insulin signalling, and metabolic and mitogenic potential.Currently published in vivo carcinogenic studies and human clinical trial data have shown that insulin glargine is not associated with increased risk for either cancer or the development or progression of diabetic retinopathy. Copyright © 2007 John Wiley & Sons, Ltd.
Genetic Study of Saudi Diabetes (GSSD): significant association of the KCNJ11 E23K polymorphism with type 2 diabetes
Authors: Alsmadi Osama , Al‐Rubeaan Khalid , Wakil Salma M. , Imtiaz Faiqa , Mohamed Gamal , Al‐Saud Haya , Al‐Saud Nouran Abu , Aldaghri Nasser , Mohammad Shahinaz , Meyer Brian F 
Published: October 2007

AbstractBackgroundThe E23K variant of KCNJ11 has been associated with type 2 diabetes (T2D) in several but not all populations studied. Thus far, despite a high incidence of T2D, the role of this variant in Arabs has not been established.MethodsWe performed a case‐control association study using 550 T2D Saudi patients (WHO criteria), and 335 controls (age ≥ 60; fasting plasma glucose < 7 mmol/L). E23K genotyping was performed by using molecular beacon‐based real time PCR assays.ResultsThe difference in K or risk allele frequency of cases and controls was significant with an OR of 1.7 (p = 0.0001). The K allele is more common among T2D patients (21%) than in the age and sex matched controls (13.6%). This was consistent with a likely eventual conversion to T2D of younger normoglycemic individuals as they grow older.ConclusionsOur results report for the first time a positive association of the E23K variant with T2D in an Arab population. Confirmation by a larger study is indicated. Copyright © 2007 John Wiley & Sons, Ltd.
PAI‐1 activity, but not fibrinogen or von Willebrand factor, is inversely related to LDL particle size in type 2 diabetes
Authors: Mertens Ilse , Lemieux Isabelle , Verrijken An , Després Jean‐Pierre , Van Gaal Luc F. 
Published: October 2007

AbstractBackgroundLevels of fibrinogen, von Willebrand factor (vWF) and plasminogen activator inhibitor‐1 (PAI‐1) have been associated with small low‐density lipoprotein (LDL) particles. However, it is not clear whether these associations are independent of visceral adiposity or other components of the metabolic syndrome such as triglycerides or insulin resistance.MethodsVisceral adipose tissue (VAT; CT‐scan), fibrinogen, von Willebrand factor antigen (vWF : Ag), PAI‐1 activity and different metabolic parameters such as total cholesterol (chol), HDL‐chol, triglycerides, insulin resistance (homeostasis model assessment; HOMA‐IR) were determined in 41 women and 78 men with type 2 diabetes. LDL particle size was assessed by polyacrylamide gradient gel electrophoresis.ResultsPAI‐1 activity was inversely related to LDL particle size after adjustment for age and body mass index (BMI) (r = − 0.28; p = 0.006) or age and VAT (r = − 0.26; p = 0.01), but not after adjustment for age and HOMA‐IR (r = − 0.15; p = 0.148) or age and triglycerides (r = − 0.04; p = 0.679). In multiple regression analysis, LDL particle size did not independently determine PAI‐1 activity levels. Fibrinogen and vWF : Ag did not seem to be related to LDL size.ConclusionsPAI‐1 activity levels, in contrast to fibrinogen and vWF : Ag, seem to be related to the small LDL phenotype in patients with type 2 diabetes. However, this relationship was not independent of insulin resistance or triglycerides. Copyright © 2007 John Wiley & Sons, Ltd.
PANDAS anorexia nervosa—Endangered, extinct or nonexistent?
Authors: Puxley Frances , Midtsund Marie , Iosif Alina , Lask Bryan 
Published: October 2007

AbstractObjective:Substantial evidence exists for the concept of pediatric autoimmune neuropsychiatric disorders associated with streptoccoccal infection (PANDAS) such as some cases of obsessive‐compulsive disorder and tics/Tourette's syndrome. More recently PANDAS‐AN has been described. The aim of this article is to provide a critical review of this concept.Method:The literature was searched using Medline, Psychinfo, and Google.Results:There is some evidence for the entity of PANDAS‐AN but this is considerably limited by a wide range of methodological problems, including the seemingly low prevalence, the lack of clarity in diagnostic criteria, and the lack of specificity in the diagnostic tests. Innovative treatment approaches hold promise but require further evaluation.Conclusion:Further research into PANDAS‐AN, using more clearly defined and consistent diagnostic criteria is needed, given the potential for enhancing the understanding of the pathogenesis of AN and for more effective treatments. © 2007 by Wiley Periodicals, Inc. Int J Eat Disord 2008
The diagnostic validity of the criteria for binge eating disorder
Authors: Latner Janet D. , Clyne Courtney 
Published: October 2007

AbstractObjective:This paper considers whether the criteria currently used to classify the diagnosis of binge eating disorder (BED) are valid and appropriate.Method:We review evidence that reflects on the validity of the current criteria for binge eating episodes and BED, using literature retrieved through major psychology and psychiatry search engines (e.g., PsycInfo, PubMed).Results:Evidence from experimental research points to the relative importance of episode frequency, the amount of food consumed at episodes, the subjective sense of loss of control over eating, and several additional criteria associated with binge episodes in BED. Evidence on the differences in psychopathology between BED and bulimia nervosa and between BED and obesity without binge eating, as related to diagnostic criteria, is reviewed.Conclusion:Although evidence concerning the diagnostic criteria of BED is mixed, broadening certain diagnostic criteria for binge eating episodes and BED might more accurately reflect the research literature and increase the number of individuals eligible for inclusion in treatment programs. © 2007 by Wiley Periodicals, Inc. Int J Eat Disord 2008