bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering influence of donor age on adipose-derived stem cells: paracrine function and angiogenic potential
Authors: TROTZIER Chloe , BELLANGER Clement , ABDESSADEQ Hakima , DELANNOY Philippe , MOJALLAL Ali , AUXENFANS Celine 
Published: February 2024

Abstract

Background: As fat grafting is commonly used as a filler, Adipose-derived stem/stromal cells (ASC) have been reported to be key player in retention rate. Paracrine and differentiation potential of those cells confer them strong pro-angiogenic capacities. However, a full characterization of the influence of aging on ASC has not been reported yet. Here we’ve investigated the effect of age on paracrine function, stemness and angiogenic potential of ASC.
Methods: ASC were extracted from young and old adult donors. We assessed stromal vascular fraction cell populations repartition, ASC stemness potential, capability to differentiate into mesenchymal lineages as well as their secretome. Angiogenic potential was assessed using a sprouting assay, an indirect co-culture of ASC and dermal microvascular endothelial cells (EC). Total vascular network length was measured, and co-culture soluble factors were quantified. Pro-angiogenic factors alone or in combination as well as ASC-conditioned medium (CM) were added to EC to assess sprouting induction.
Results: Decrease of endothelial cells yield and percentage is observed in cells extracted from adipose tissue of older patients, whereas ASC percentage increased with age. Clonogenic potential of ASC is stable with age. ASC can differentiate into adipocytes, chondrocytes and osteoblasts, and aging does not alter this potential. Among the 25 analytes quantified, high levels of pro-angiogenic factors were found, but none is significantly modulated with age. ASC induce a significantly longer vascular network compared to fibroblasts, and no difference was found between young and old ASC donors on that parameter. Higher concentrations of bFGF, G-CSF, HGF and IL-8, and lower concentrations of VEGF-C were quantified in EC/ASC co-cultures compared to EC/fibroblasts co-cultures. EC/ASC from young donors secrete higher levels of VEGF-A compared to old ones. Neither soluble factor nor CM without cells are able to induce organized sprouts, highlighting the requirement of cell communication for sprouting. CM of ASC donor inducing long vascular network can restore pro-angiogenic potential of ASC donor inducing short vascular network.
Conclusion: Our results show cells from young and old donors exhibit no difference in all assessed parameters, suggesting all patients could be included in clinical applications. We emphasized the leading role of ASC in angiogenesis process, without impairment with age, but further studies are required to better understand the mechanistic of this phenomenon.
Wavelet-based Arcing Signal Source Localization Algorithm using a Compact Multi-square Microstrip Antenna
Authors: Amirruddin Melaty , Adzman Mohd Rafi , Affendi Nur Adyani Mohd , Isa Muzamir , Idris Muhd Hafizi , Halim Syahirah Abd , Lehtonen Matti 
Published: February 2024

Abstract

Ensuring power system safety involves effective arc fault detection and localization. Existing devices struggle to differentiate normal and abnormal conditions, especially in confined spaces, posing precision challenges. Placing antennas strategically around the arc aids in detecting electromagnetic radiation, even in limited areas. This enables valuable data collection for real-time monitoring. To address these challenges, this paper proposes integrating experimental work using a compact multi-square microstrip antenna and signal processing techniques. The study compares the effectiveness of three signal processing approaches: threshold, Discrete Wavelet Transform (DWT), and Continuous Wavelet Transform (CWT). The signal processing technique separates genuine arc signals from background noise by identifying unique characteristics and isolating the dominant frequency. The Time of Arrival (ToA) is measured and used in Least Square (LS) and Gauss-Jordan Elimination (GJE) methods to calculate the arc source location. The outcomes illustrate the precision of the proposed model in detecting and pinpointing arc source signals, with error margins ranging from 6.15% to 7.13% for the CWT technique, 6.88% to 7.89% for the DWT technique, and 7.99% to 8.44% for the threshold technique. These results hold promise for enhancing the integration of experimental approaches in assessing arcing conditions, thereby addressing challenges in insulation systems.
Accuracy of self-collected versus healthcare worker collected specimens for diagnosing sexually transmitted infections: an updated systematic review and meta-analysis
Authors: Jaya Ziningi , Mapanga Witness , Dlangalala Thobeka , Thembane Nokukhanya , Kgarosi Kabelo , Dzinamarira Tafadzwa , Mashamba-Thompson Tivani 
Published: February 2024

Abstract

The use of self-collected specimens as an alternative to healthcare worker-collected specimens for diagnostic testing has gained increasing attention in recent years. This systematic review aimed to assess the diagnostic accuracy of self-collected specimens compared to healthcare worker-collected specimens across different sexually transmitted infections (STIs) including chlamydia, human papillomavirus (HPV), Mycoplasma genitalium (MG), gonorrhoea, syphilis and trichomoniasis in females. A rigorous process was followed to screen for studies in various electronic databases. The quality of included studies was assessed using the Quality Assessment of Diagnostic Accuracy Studies 2 tool. There were no studies on syphilis that met the criteria for inclusion in the review. A total of six studies for chlamydia, five studies for HPV, four studies for MG, and seven studies for gonorrhoea and trichomoniasis were included in the review. However, not all studies were included in the sub-group meta-analysis. The analysis revealed that self-collected specimens demonstrated comparable diagnostic accuracy to healthcare worker-collected specimens across most STIs. This indicates that the diagnostic accuracy of self-collected specimens can provide accurate results and enhance access to diagnostic testing, potentially improving healthcare service delivery. Future research should further explore the diagnostic accuracy of self-collected specimens in larger and more diverse populations.
Changes in Motor and Sensory Cortical Neurotransmitters Following Traumatic Brachial Plexus Injuries in Adults Measured with Magnetic Resonance Spectroscopy
Authors: Wade Ryckie G , Bourke Gráinne , Olaru Alexandra M , Williams Steve R , Shelley David , Plein Sven , Bains Robert D , Bedford James D , Newton Lucy Homer , Ng Chye Yew , Parkes Laura , Lea-Carnall Caroline 
Published: February 2024

Abstract
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. In response to injury within the central nervous system, GABA promotes cortical plasticity and represents a potential pharmacological target to improve functional recovery. However, it is unclear how GABA changes in the brain after traumatic brachial plexus injuries (tBPI). Therefore, neurometabolite concentrations were serially quantified in 7 males with acute tBPI, up to 19 months post-injury, using magnetic resonance spectroscopy (MRS). We acquired J-difference editing MRS using a MAGNETOM Prisma 3T (Siemens Healthcare, Erlangen, Germany). Data were analysed in jMRUI blind to clinical information to quantify GABA, GABA, creatine (Cr) and N-acetylaspartate (NAA) concentrations. Interhemispheric means were compared using linear methods. Confidence intervals (CI) were generated to the 95% level. Within weeks of injury, the hemisphere representing the injured upper limb had a significantly lower GABA:NAA ratio (mean difference 0.23 [CI 0.06–0.40]) and GABA:Cr ratio (mean difference 0.75 [CI 0.24–1.25]) than the uninjured side, which equalised 12 months post-injury. After BPI, there are interhemispheric differences in GABA concentrations within the sensory and motor cortex. Pharmacological modulation of these metabolites may enhance cortical plasticity, prolong the available time for reconstructive surgery and ultimately, improve function.
Activity-stability trade-off observed in variants at position 315 of the GH10 xylanase XynR
Authors: Nakamura Tomoka , Takita Teisuke , Kuwata Kohei , Mizutani Kimihiko , Mikami Bunzo , Nakamura Satoshi , Yasukawa Kiyoshi 
Published: February 2024

Abstract
XynR is a thermostable alkaline GH10 xylanase, for which we have previously examined the effects of saturation mutagenesis at position 315 on enzyme alkaliphily, and found that at pH 10, the activities of variants could be ordered as follows: T315Q > T315S = T315N > T315H = wild-type XynR (WT) > 15 other variants. In this study, we sought to elucidate the mechanisms underlying the variable activity of these different variants. Crystallographic analysis revealed that the Ca2+ ion near position 315 in WT was absent in the T315Q variant. We accordingly hypothesized that the enhancement of alkaliphily in T315Q, and probably also in the T315H, T315N, and T315S variants, could be ascribed to an activity-stability trade-off associated with a reduction in stability due to the lack of this Ca2+ ion. Consistent with expectations, the alkaline resistance of T315H, T315N, T315Q, and T315S, evaluated through the pH-dependence of stability at 0 mM CaCl2 under alkaline conditions, was found to be lower than that of WT. In addition, the thermostabilities of these four variants, as assessed using the denaturing temperatures (Tm) at 0 mM CaCl2 based on ellipticity at 222 nm in circular dichroism measurements, were lower than that of WT. Furthermore, the Tm values of WT and variants at 5 mM CaCl2 were higher than those at 0 mM CaCl2 by 6–11°C. Collectively, our findings in this study indicate that mutation of the T residue at position 315 of XynR to Q, S, N, and S causes an increase in the alkaliphily of this enzyme, thereby reducing its stability.
DTRoadseg: A duplex transform heterogeneous feature fusion network for road segmentation
Authors: Guo Zhiyang , Hu Xing , Wang Jiejia , Miao XiaoYu , Sun MengTeng , Wang HuaiWei , Ma XueYing 
Published: February 2024

Abstract

Detecting roads in automatic driving environments poses a challenge due to issues such as boundary fuzziness, occlusion, and glare from light. We believe that two factors are instrumental in addressing these challenges and enhancing detection performance: global context dependency and effective feature representation that prioritizes important feature channels. To tackle these issues, we introduce DTRoadseg, a novel duplex Transformer-based heterogeneous feature fusion network designed for road segmentation. DTRoadseg leverages a duplex encoder architecture to extract heterogeneous features from both RGB images and point-cloud depth images. Subsequently, we introduce a multi-source Heterogeneous Feature Reinforcement Block (HFRB) for fusion of the encoded features, comprising a Heterogeneous Feature Fusion Module (HFFM) and a Reinforcement Fusion Module (RFM). The HFFM leverages the self-attention mechanisms of Transformers to achieve effective fusion through token interactions, while the RFM focuses on emphasizing informative features while downplaying less important ones, thereby reinforcing feature fusion. Finally, a Transformer decoder is utilized to produce the final semantic prediction. Furthermore, we employ a boundary loss function to optimize the segmentation structure area, reduce false detection areas, and improve model accuracy. Extensive experiments are carried out on the KITTI road dataset. The results demonstrate that, compared with state-of-the-art methods, DTRoadseg exhibits superior performance in terms of segmentation accuracy.
Identification of a predictive model for schizophrenia based on SNPs in a Chinese population
Authors: 杨 志英 , 姚明 顺 , 徐 伊冲 , 张 小清 , 什 元 , 王 丽君 , 崔 东红 
Published: February 2024

Abstract
Background
Schizophrenia is a devastating mental disease with high heritability. A growing number of susceptibility genes associated with schizophrenia, as well as their corresponding SNPs loci, have been revealed by genome-wide association studies. However, using SNPs as predictors of disease and diagnosis remains difficult. Here, we aimed to uncover susceptibility SNPs in a Chinese population and to construct a prediction model for schizophrenia.
Methods
A total of 210 participants, including 70 patients with schizophrenia, 70 patients with bipolar disorder, and 70 healthy controls, were enrolled in this study. We estimated 14 SNPs using published risk loci of schizophrenia, and used these SNPs to build a model for predicting schizophrenia via comparison of genotype frequencies and logistic regression. We evaluated the efficacy of the diagnostic model in schizophrenia and control patients using ROC curves and then used the 70 patients with bipolar disorder to evaluate the model’s differential diagnostic efficacy.
Results
5 SNPs were selected to construct the model: rs148415900, rs71428218, rs4666990, rs112222723 and rs1716180. Correlation analysis results suggested that, compared with the risk SNP of 0, the risk SNP of 3 was associated with an increased risk of schizophrenia (OR = 13.00, 95%CI:2.35–71.84, P = 0.003). The ROC-AUC of this prediction model for schizophrenia was 0.719 (95% CI: 0.634–0.804), with the greatest sensitivity and specificity being 60% and 80%, respectively. The ROC-AUC of the model in distinguishing between schizophrenia and bipolar disorder was 0.591 (95%CI: 0.497–0.686), with the greatest sensitivity and specificity being 60% and 55.7%, respectively.
Conclusions
The SNP risk score prediction model had good performance in predicting schizophrenia. To the best of our knowledge, previous studies have not applied SNP-based models to differentiate between cases of schizophrenia and other mental illnesses. It could have several potential clinical applications, including shaping disease diagnosis, treatment, and outcomes.
A climatology of stratospheric gravity waves induced by tropical cyclones
Authors: Zhang Lifeng , Wang Xu , Wang Yuan , Zhang Yun , Guan Jiping 
Published: February 2024

Abstract

A climatology of stratospheric gravity waves (SGWs) induced by tropical cyclones (TC-SGWs) is necessary for stratospheric aviation safety and accurate numerical weather prediction. Few previous works have characterized the climatology of TC-SGWs, and the relative importance of the source and background wind for TC-SGWs has not been clearly established. Here we present the distribution of TC-SGWs formed over the northwest Pacific Ocean based on 30 years of ERA5 reanalysis data. Using explainable artificial intelligence, we found the background wind in the lower stratosphere to be more important than TCs in controlling the distribution pattern of TC-SGWs. The background wind shear can influence the direction of TC-SGW propagation by refraction and filtering, which influences the distribution pattern of the TC-SGWs. The TC intensity only influences the TC-SGW intensity. Our results provide information on TC-SGW hotspots and suggest the importance of the background wind shear.
Investigation of Nicotine Dependence in Young Male Soldiers After High-altitude Exposure: a Cross- Sectional Study
Authors: Xiao Zhong-Ming , Chen Yu , Ma Ke , Li Shao-Ying , Men Bo , Yu Ting , Nian Zi-Xiong , He Gong-Hao , Zhang Xin , Cai Wen-Ke 
Published: February 2024

Abstract

This study was conducted as a cross-sectional survey using a questionnaire to analyze "demographic characteristics, nicotine dependence, and self-efficacy for smoking cessation" among healthy young adult male soldiers who are presently residing and training in high-altitude for a minimum period of 3 months. A total of 3307 questionnaires were distributed between January and August 2023 in the high-altitude areas (altitude approximately 1700m-3700m).The participants in the survey exhibited an average age of 24.5 ± 3.7 years. A notable 82% of the respondents migrated to the high-altitude from regions with an altitude below 1500m, while 12.5% of smokers had resided and worked in the high-altitude area for a duration of ≥6 months. A substantial 89.6% of participants shared living spaces with smoking roommates; 60.1% initiated smoking before reaching the age of 19; 75% of smokers maintained a smoking habit spanning 1-9 years; a predominant 94.2% manifested very low to moderate nicotine dependence, with the remaining 5.8% presenting high to very high dependence. A distinct correlation emerged between factors such as BMI, duration of residence and work at high altitudes, daily smoking quantity (in the last 6 months), maximum daily smoking amount, roommate smoking status, and smoking duration, and the heightened status of nicotine dependence. An impressive 82.1% of respondents conveyed a resolute intent to cease smoking, with pivotal determinants such as educational attainment, daily smoking quantity (in the last month), maximum daily smoking amount, and smoking duration significantly influencing the cessation intention. Nicotine dependence persists in young adult male soldier smokers post high-altitude exposure, with the majority expressing a strong desire to quit. Therefore, our focus should be on bolstering their willingness to quit, crafting a tailored cessation program, and elevating the success rate of smoking cessation.
Induced Erotomania by Online Romance Fraud - A Novel Form of de Clérambault's Syndrome
Authors: Alotti Nasri , Osvath Peter , Tenyi Tamas , Voros Viktor 
Published: February 2024

Abstract
Background: Although the impact of internet usage on mental health is extensively documented, there is a notable scarcity of reports in the literature concerning cyber-induced Erotomania. Erotomania is a rare but likely underdiagnosed delusional disorder. It is characterized by an irrational belief held by the affected persons that someone of higher socioeconomic status harbor romantic feelings toward them. Here, we describe and summarize the psychopathology of Erotomanic delusion induced by online romantic fraud in a female patient. Employing this case as a focal point, we illuminate novel aspects of erotomania that warrant attention and examination.
Case presentation: We present a compelling case involving a 70-year-old married Caucasian woman diagnosed with medically controlled persistent depressive disorder for several years. The intricacies of her condition became evident as she became deeply engrossed in online profiles featuring the image of a renowned musician, inadvertently falling victim to an online romantic fraud. Subsequently, this distressing experience triggered the emergence of erotomanic delusions and a suicide attempt. The patient's history reveals an array of medical conditions and stressful life events, contributing to her vulnerability. The diagnosis of erotomanic delusional disorder, dysthymia, and mild cognitive impairment with cerebral vascular background was established. Treatment involved her previous antidepressant with low-dose risperidone, alongside supportive individual and group therapy. Her delusion showed remission four weeks later, prompting her discharge for outpatient follow-up. Although she retained some false beliefs, the intensity of the symptoms had notably diminished and her functionality improved.
Conclusions: This case underscores the complex interplay between mental health, online activities, and the consequences of delusions, including suicidal thoughts, shedding light on the need for a comprehensive approach in addressing such challenging psychiatric scenarios.
Association between education inequality and lung cancer: a two- sample Mendelian randomization study
Authors: Nian Hui , Chen Xiaosang , Xu Songtao 
Published: February 2024

Abstract

Background: While numerous studies have explored the correlation between educational disparities and lung cancer, the definitive determination of a causal link between these factors is still pending. This study seeks to clarify the potential causal role of educational disparities in the development of lung cancer.
Methods: This study employed a two-sample Mendelian randomization (MR) analysis by using genetic data from genome-wide association studies (GWAS) to explore the hypothesized causal connection between educational attainment and lung cancer. The investigation specifically concentrated on its major subtypes: Lung Squamous Cell Carcinoma (LUSC) and Lung Adenocarcinoma (LUAD). In order to enhance our understanding and address potential confounding factors, a Multivariable MR analysis was utilized. Additionally, an advanced Two-step MR methodology was applied to clarify the biological pathways through which educational attainment might impact the risk of lung cancer.
Results: This study employed the Multiplicative Random-Effect Inverse Variance Weighted (IVW-MRE) as primary method in Univariable Mendelian Randomization (UVMR) analysis. The IVW-MRE approach yielded MR estimates indicating an odds ratio (OR) of 0.153 (95% Confidence Interval [CI]: 0.125-0.186) for overall Lung Cancer (LUCA), 0.195 (95% CI: 0.154–0.246) for Lung Squamous Cell Carcinoma (LUSC), and 4.860 (95% CI: 3.584–6.589) for Lung Adenocarcinoma (LUAD). Additionally, Multivariable Mendelian Randomization (MVMR) analysis confirmed the persistent significance of educational inequalities as risk determinants. A subsequent two-step MR analysis further delineated the mediating roles of smoking, alcohol consumption, and Body Mass Index (BMI) in this association, and only found alcohol consumption has mediation effect between the two traits.
Conclusion: Our findings indicate a genetic connection between educational inequality and susceptibility to lung cancer. This emphasizes the need for more extensive research to unravel the complex socio-genetic interactions involved. Importantly, these results underscore the crucial role of addressing educational disparities in the epidemiology and progression of lung cancer.
The influencing factors of tourists’ behavioral intention in City walk in Malaysia
Authors: wang zhenbin , Zhang Hui , Cao Chaoyi 
Published: February 2024

Abstract
The study aims to fill the gaps in theoretical studies of the new travel trend of city walk in Malaysia from the perspective of embodiment with factors of sensory experience, visitor engagement, authenticity, and behavioral intention. Memorable tourism experiences and perceived risk are mediators and moderators between embodied cognition and behavioral intention, MTEs, and behavioral intention. Chinese tourists who are the major international tourist in Malaysia were taken into the investigation. The results show that sensory experience and existential authenticity have a positive effect on behavioral intention, MTEs play a mediating role in the relationships of sensory experience, authenticity, and behavioral intention. there is no significant mediation effect between visitor engagement and behavioral intention through MTEs. Perceived risk also shows no significant moderating effect on behavioral intention in city walk in Malaysia. This study provides new conceptual frameworks and research perspectives on City walk in Malaysia with theoretical and practical contributions leading to further study and city tour development practices.