bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactive Sulforaphane from in vitro Propagated Brassica Seedlings
Authors: Sivakumar G. , Aliboni A. , Antonini A. , Bacchetta L. 
Published: May 2007

AbstractSulforaphane is a biologically active phytochemical found abundantly in Brassica species. Sulforaphane from eighteen samples of three‐day‐old in vitro seedlings from Brassicas, the Brassica oleracea and Brassica rapa varieties were analyzed by HPLC. San Martino, cavolfiore Alverda, cavolo verza riccio d'Asti and cavolfiore Minaret (4.57; 4.54; 4.53 and 4.53 [mg/g d.w], respectively) seedlings showed the highest level of sulforaphane. The presented data may be applicable to choose sulforaphane‐rich Brassica varieties for functional foods. A higher sulforaphane content in functional foods might offer health benefits or desirable physiological effects.
Order imbalance and the pricing of index futures
Authors: Fung Joseph K.W. 
Published: May 2007

AbstractThis study examines whether the aggregate order imbalance for index stocks can explain the arbitrage spread between index futures and the underlying cash index. The study covers the period of the Asian financial crisis and includes wide variations in order imbalance and the indexfutures basis. The analysis controls for realistic trading costs and actual dividend payments. The results indicate that the arbitrage spread is positively related to the aggregate order imbalance in the underlying index stocks; negative order‐imbalance has a stronger impact than positive order imbalance. Violations of the upper no‐arbitrage bound are related to positive order imbalance; of the lower no‐arbitrage bound to negative order imbalance. Asymmetric response times to negative and positive spreads can be attributed to the difficulty, cost, and risk of short stock arbitrage when the futures are below their no‐arbitrage value. The significant relationship between order imbalance and arbitrage spread confirms that index arbitrageurs are important providers of liquidity in the futures market when the stock market is in disequilibrium. © 2007 Wiley Periodicals, Inc. Jrl Fut Mark 27:697–717, 2007
Role of phospholipase D2 in anti‐apoptotic signaling through increased expressions of Bcl‐2 and Bcl‐xL
Authors: Oh Kyoung‐Jin , Lee Sung‐Chang , Choi Hye‐Jin , Oh Doo‐Yi , Kim Sang‐Chul , Min Do Sik , Kim Jung Mogg , Lee Ki Sung , Han Joong‐Soo 
Published: May 2007

AbstractWe have previously reported that Fas‐resistant A20 cells (FasR) have phospholipase D (PLD) activity upregulated by endogenous PLD2 overexpression. In the present study, we investigated how overexpressed PLD2 in FasR could generate survival signals by regulating the protein levels of anti‐apoptotic Bcl‐2 and Bcl‐xL. To confirm the effect of PLD2 on Bcl‐2 protein levels, we transfected PLD2 into wild‐type murine B lymphoma A20 cells. The transfected cells showed markedly the increases in Bcl‐2 and Bcl‐xL protein levels, and became resistant to Fas‐induced apoptosis, similar to FasR. Treatment of wild‐type A20 cells with phosphatidic acid (PA), the metabolic end product of PLD2 derived from phosphatidylcholin, markedly increased levels of anti‐apoptotic Bcl‐2 and Bcl‐xL proteins. Moreover, PA‐induced expressions of Bcl‐2 and Bcl‐xL were enhanced by propranolol, an inhibitor of PA phospholydrolase (PAP), whereas completely blocked by mepacrine, an inhibitor of phospholipase A2 (PLA2), suggesting that PLA2 metabolite of PA is responsible for the increases in Bcl‐2 and Bcl‐xL protein levels. We further confirmed the involvement of arachidonic acid (AA) in PA‐induced survival signals by showing that 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphate (DPPA), PA without AA, was unable to increase Bcl‐2 and Bcl‐xL proteins. Moreover, PA notably increased cyclooxygenase (COX)‐2 protein expression, and PA‐induced expression of both Bcl‐2 and Bcl‐xL was inhibited by NS‐398, a specific inhibitor of COX‐2. Taken together, these findings demonstrate that PA generated by PLD2 plays an important role in cell survival during Fas‐mediated apoptosis through the increased Bcl‐2 and Bcl‐xL protein levels which resulted from PLA2 and AA‐COX2 pathway. J. Cell. Biochem. 101: 1409–1422, 2007. © 2007 Wiley‐Liss, Inc.
The proteasome regulates the interaction between Cx43 and ZO‐1
Authors: Girao Henrique , Pereira Paulo 
Published: May 2007

AbstractGap junction (GJ) intercellular communication (GJIC) is vital to ensure proper cell and tissue function. GJ are multimeric structures composed of proteins called connexins. Modifications on stability or subcellular distribution of connexins have a direct impact on the extent of GJIC. In this study we have investigated the role of the proteasome in regulation of connexin 43 (Cx43) internalization. Although the participation of both the proteasome and lysosome has long been suggested in Cx43 degradation, the molecular mechanisms whereby proteasome contributes to regulate Cx43 internalization and intercellular communication are still unclear. The results presented in this study envision a new mechanism whereby proteasome regulates GJIC by modulating interaction between Cx43 and ZO‐1. Immunoprecipitation experiments, in the presence of proteasome inhibitors, together with immunofluorescence data indicate that the proteasome regulates interaction between Cx43 and ZO‐1. Overexpression of the PDZ2 domain of ZO‐1 and the expression of Cx‐43 fused in frame with a V5/HIS tag, suggest that interaction between the two proteins occurs through the PDZ2 domain of ZO‐1 and the C‐terminus of Cx43. When interaction between Cx43 and ZO‐1 is reduced, as in the presence of proteasome inhibitors, Cx43 accumulates, forming large GJ plaques at plasma membrane. Data presented in this article suggest a new pathway whereby alterations in proteasome activity may impact on GJIC as well as on non‐junctional communication with extracellular environment, contributing to cell and tissue dysfunction. J. Cell. Biochem. 102: 719–728, 2007. © 2007 Wiley‐Liss, Inc.
Co‐inoculation of prostate cancer cells with U937 enhances tumor growth and angiogenesis in vivo
Authors: Craig Matthew , Ying Chi , Loberg Robert D. 
Published: May 2007

AbstractTumor‐associated macrophages (TAMs) have been implicated in promoting tumor growth and development. Here we present evidence that demonstrates that co‐inoculation of male athymic nude mice with PC‐3 prostate cancer cells and U937 promonocytic cells enhances tumor growth and increases tumor angiogenesis.
Male athymic nude mice were co‐inoculated with PC‐3 and U937 cells (control or IL‐4 stimulated) and tumor growth was monitored over time. Immunohistochemical analysis of tumor specimens was performed for proliferation markers (e.g., Ki67) and the effects of IL‐4 stimulation on U937 cells were analyzed for chemokine expression.
The presence of U937 cells increased the rate of tumor growth in vivo and stimulated increased microvascular density within the tumor bed. Stimulation of U937 cells with IL‐4 resulted in a significant increase in several pro‐angiogenic and pro‐tumor chemokines (e.g., CCL2).
Co‐inoculation increases prostate cancer growth via upregulation of chemokines that induce angiogenesis within the tumor. J. Cell. Biochem. 103: 1–8, 2008. © 2007 Wiley‐Liss, Inc.
Intranuclear mobility of estrogen receptor α and progesterone receptors in association with nuclear matrix dynamics
Authors: Matsuda KenIchi , Nishi Mayumi , Takaya Hisamitsu , Kaku Natsuko , Kawata Mitsuhiro 
Published: May 2007

AbstractWe analyzed the intranuclear dynamics of estrogen receptor α (ERα) and progesterone receptor (PR)‐A/B labeled with different spectral variants of green fluorescent protein (GFP) in living cells. The distribution of ERα and PR‐A/B were changed from a diffuse to discrete pattern after the addition of both ligands, but the extent of discrete cluster formation of PR‐A/B was lower than that of ERα. The nuclear areas where PR‐A/B were accumulated were colocalized with the cluster of ERα, suggesting that cross‐talk in the transcriptional regulation occurred in the loci. Fluorescence recovery after photobleaching (FRAP) analysis revealed that the mobility of PR‐A/B was hastened by the coexistence of ERα, while the mobility of ERα was not changed by the coexistence of PR‐A/B. Cluster formation was correlated with the nuclear matrix binding, because nuclear matrix binding capacity was also lower in PR‐A/B than ERα. By ATP‐depletion from the cells, most of ERα and PR‐A/B were bound to the nuclear matrix and their mobilities were extinguished both in the absence and presence of ligand. Fluorescent protein (FP) tagged nuclear matrix component protein (NuMA), which was colocalized with ERα and PR‐A/B, showed ATP‐dependent rapid exchange in the nucleus. These results indicate that the mobility of ERα and PR‐A/B is associated with the dynamics of the nuclear matrix. J. Cell. Biochem. 103: 136–148, 2008. © 2007 Wiley‐Liss, Inc.
Matrigel‐induced acinar differentiation is followed by apoptosis in HSG cells
Authors: Szlávik Vanda , Vág János , Markó Károly , Demeter Kornél , Madarász Emília , Oláh Imre , Zelles Tivadar , O'Connell Brian C. , Varga Gábor 
Published: May 2007

AbstractIt has been shown that a human salivary gland cell line (HSG) is capable of differentiation into gland‐like structures, though little is known of how morphological features are formed or controlled. Here we investigated the changes in cell proliferation and apoptosis upon terminal differentiation of HSG cells in Matrigel, an extracellular matrix derivative. Changes in the expression of survivin, a prominent anti‐apoptotic factor, and caspase‐3, a key apoptotic factor were also measured. In order to better understand the involvement of key signal transduction pathways in this system we pharmacologically blocked the activity of tyrosine kinase, nuclear factor kappa B(NFκB), protein kinase C (PKC), phosphatidylinositol 3‐kinase (PI3K) and matrix metalloproteases (MMP). Results of these studies demonstrate that cytodifferentiation of HSG cells to an acinar phenotype is accompanied first by a decrease of cell proliferation and then by a massive programmed cell death, affected by multiple signal transduction pathways. Thus, Matrigel alone is insufficient for the full maturation and long term survival of the newly formed acini: the presence of other factors is necessary to complete the acinar differentiation of HSG cells. J. Cell. Biochem. 103: 284–295, 2008. © 2007 Wiley‐Liss, Inc.
Yeast Nα‐terminal acetyltransferases are associated with ribosomes
Authors: Polevoda Bogdan , Brown Steven , Cardillo Thomas S. , Rigby Sean , Sherman Fred 
Published: May 2007

AbstractN‐terminal acetylation is one of the most common modifications, occurring on the vast majority of eukaryotic proteins. Saccharomyces cerevisiae contains three major NATs, designated NatA, NatB, and NatC, with each having catalytic subunits Ard1p, Nat3p, and Mak3p, respectively. Gautschi et al. (Gautschi et al. [2003] Mol Cell Biol 23: 7403) previously demonstrated with peptide crosslinking experiments that NatA is bound to ribosomes. In our studies, biochemical fractionation in linear sucrose density gradients revealed that all of the NATs are associated with mono‐ and polyribosome fractions. However only a minor portion of Nat3p colocalized with the polyribosomes. Disruption of the polyribosomes did not cause dissociation of the NATs from ribosomal subparticles. The NAT auxiliary subunits, Nat1p and Mdm20p, apparently are required for efficient binding of the corresponding catalytic subunits to the ribosomes. Deletions of the genes corresponding to auxiliary subunits significantly diminish the protein levels of the catalytic subunits, especially Nat3p, while deletions of the catalytic subunits produced less effect on the stability of Nat1p and Mdm20p. Also two ribosomal proteins, Rpl25p and Rpl35p, were identified in a TAP‐affinity purified NatA sample. Moreover, Ard1p copurifies with Rpl35p‐TAP. We suggest that these two ribosomal proteins, which are in close proximity to the ribosomal exit tunnel, may play a role in NatA attachment to the ribosome. J. Cell. Biochem. 103: 492–508, 2008. © 2007 Wiley‐Liss, Inc.
The role of androgen in determining differentiation and regulation of androgen receptor expression in the human prostatic epithelium transient amplifying population
Authors: Heer R. , Robson C.N. , Shenton B.K. , Leung H.Y. 
Published: May 2007

AbstractAbnormal differentiation in epithelial stem cells or their immediate proliferative progeny, the transiently amplifying population (TAP), may explain malignant pathogenesis in the human prostate. These models are of particular importance as differing sensitivities to androgen among epithelial cell subpopulations during differentiation are recognised and may account for progression to androgen independent prostate cancer. Androgens are crucial in driving terminal differentiation and their indirect effects via growth factors from adjacent androgen responsive stroma are becoming better characterised. However, direct effects of androgen on immature cells in the context of a prostate stem cell model have not been investigated in detail and are studied in this work. In α2β1hi stem cell enriched basal cells, androgen analogue R1881 directly promoted differentiation by the induction of differentiation‐specific markers CK18, androgen receptor (AR), PSA and PAP. Furthermore, treatment with androgen down‐regulated α2β1 integrin expression, which is implicated in the maintenance of the immature basal cell phenotype. The α2β1hi cells were previously demonstrated to lack AR expression and the direct effects of androgen were confirmed by inhibition using the anti‐androgen bicalutamide. AR protein expression in α2β1hi cells became detectable when its degradation was repressed by the proteosomal inhibitor MG132. Stratifying the α2β1hi cells into stem (CD133+) and transient amplifying population (TAP) (CD133−) subpopulations, AR mRNA expression was found to be restricted to the CD133− (TAP) cells. The presence of a functional AR in the TAP, an androgen independent subpopulation for survival, may have particular clinical significance in hormone resistant prostate cancer, where both the selection of immature cells and functioning AR regulated pathways are involved. J. Cell. Physiol. 212:572–578, 2007. © 2007 Wiley‐Liss, Inc.
LRRC4 inhibits glioblastoma cell proliferation, migration, and angiogenesis by downregulating pleiotropic cytokine expression and responses
Authors: Wu Minghua , Huang Chen , Li Xiayu , Li Xiaoling , Gan Kai , Chen Qiong , Tang Yunlian , Tang Ke , Shen Shourong , Li Guiyuan 
Published: May 2007

AbstractLeucine‐rich repeat C4 (LRRC4) has been shown to inhibit glioma cell proliferation, however, little is known about the mechanism(s) underlying the action of LRRC4. Here, we show that two glioblstoma U251 cell clones stably expressing LRRC4 were established. LRRC4 expression significantly inhibited the expression of some cytokines and their receptors determined by microarray and Western blot assays, and dramatically reduced cytokine‐induced AP‐1, NF‐kB, and CyclinD1 activation in glioma cells. Furthermore, LRRC4 expression in glioma cells significantly downregulated spontaneous and cytokine‐induced expression of K‐RAS and phosphorylation of c‐Raf, ERK, AKT, NF‐kBp65, p70S6K, and PKC, suggesting that LRRC4 inhibited receptor tyrosine kinase (RTK) signaling pathways. Moreover, treatment with bFGF, IGF1, or IGF2 stimulated LRRC4−/−, but not the LRRC4+, glioma cell proliferation, indicating that LRRC4 mitigated cytokine‐stimulated proliferation in glioma cells. In addition, treatment of LRRC4−/− glioma cells with EGF, IGF2, or PDGF promoted long distance mobilization, but induced little migration in LRRC4+ glioma cells, suggesting that LRRC4 retarded cytokine‐promoted glioma cell migration in vitro. Finally, human vessel endothelial cells (ECV304) treated with VEGF grew, aligned and formed hollow tube‐like structures in vitro. In contrast, LRRC4+ ECV304 treated with VEGF failed to form vessel‐tube structures. Collectively, LRRC4 expression inhibited the expression of some growth factors, cytokines and their receptors, and the capacity of glioma cells responding to cytokine stimulation, leading to inhibition of glioma cell proliferation. Conceivably, induction of LRRC4 expression may provide new intervention for human glioma in the clinic. J. Cell. Physiol. 214:65–74, 2008. © 2007 Wiley‐Liss, Inc.
Integration of wings and their eyespots in the speckled wood butterflyPararge aegeria
Authors: Breuker Casper J. , Gibbs Melanie , Van Dyck Hans , Brakefield Paul M. , Klingenberg Christian Peter , Van Dongen Stefan 
Published: May 2007

AbstractWe investigated both the phenotypic and developmental integration of eyespots on the fore‐ and hindwings of speckled wood butterfliesPararge aegeria. Eyespots develop within a framework of wing veins, which may not only separate eyespots developmentally, but may at the same time also integrate them by virtue of being both signalling sources and barriers during eyespot development. We therefore specifically investigated the interaction between wing venation patterns and eyespot integration. Phenotypic covariation among eyespots was very high, but only eyespots in neighbouring wing cells and in homologous wing cells on different wing surfaces were developmentally integrated. This can be explained by the fact that the wing cells of these eyespots share one or more wing veins. The wing venation patterns of fore‐ and hindwings were highly integrated, both phenotypically and developmentally. This did not affect overall developmental integration of the eyespots. The adaptive significance of integration patterns is discussed and more specifically we stress the need to conduct studies on phenotypic plasticity of integration.J. Exp. Zool. (Mol. Dev. Evol.) 308B:454–463, 2007. © 2007 Wiley‐Liss, Inc.
Improving fragmentation of poorly fragmenting peptides and phosphopeptides during collision‐induced dissociation by malondialdehyde modification of arginine residues
Authors: Leitner Alexander , Foettinger Alexandra , Lindner Wolfgang 
Published: May 2007

AbstractDespite significant technological and methodological advancements in peptide sequencing by mass spectrometry, analyzing peptides that exhibit only poor fragmentation upon collision‐induced dissociation (CID) remains a challenge. A major cause for unfavorable fragmentation is insufficient proton ‘mobility’ due to charge localization at strongly basic sites, in particular, the guanidine group of arginine. We have recently demonstrated that the conversion of the guanidine group of the arginine side chain by malondialdehyde (MDA) is a convenient tool to reduce the basicity of arginine residues and can have beneficial effects for peptide fragmentation. In the present work, we have focused on peptides that typically yield incomplete sequence information in CID‐MS/MS experiments. Energy‐resolved tandem MS experiments were carried out on angiotensins and arginine‐containing phosphopeptides to study in detail the influence of the modification step on the fragmentation process. MDA modification dramatically improved the fragmentation behavior of peptides that exhibited only one or two dominant cleavages in their unmodified form. Neutral loss of phosphoric acid from phosphopeptides carrying phosphoserine and threonine residues was significantly reduced in favor of a higher abundance of fragment ions. Complementary experiments were carried out on three different instrumental platforms (triple‐quadrupole, 3D ion trap, quadrupole–linear ion trap hybrid) to ascertain that the observation is a general effect. Copyright © 2007 John Wiley & Sons, Ltd.
Metastatic bone disease: A 36‐Year single centre trend‐analysis of patients admitted to a tertiary orthopaedic surgical department
Authors: Toma C.D. , Dominkus M. , Nedelcu T. , Abdolvahab F. , Assadian O. , Krepler P. , Kotz R. 
Published: May 2007

AbstractBackground and ObjectivesThe treatment and outcome of primary malignant bone tumours has changed with the advances in diagnostic and treatment modalities. A trend‐analysis on a large cohort of patients with metastatic bone disease was performed.MethodsA retrospective chart review of all cases with metastatic bone disease admitted to a single tertiary orthopaedic referral centre, registered with the Vienna Bone and Soft Tissue Tumour Registry between 1968 and 2003 was conducted. For trend‐analysis of frequency, survival, primary site, treatment methods, and others, the 36‐year study duration was divided into four periods.ResultsThe study identified 601 females and 580 males (mean: 60 years) with metastatic bone disease. The most common metastases were secondary to breast cancer (n = 275; 23%) and renal cell carcinoma (n = 242; 21%) and the majority were located in the femur (n = 332; 28%) and spine (n = 348; 29%). Overall, the proportion of patients who underwent surgery decreased. At follow‐up, 887 (75%) patients were verified to have died of their disease.ConclusionsOver the 36‐year period, the frequency of bone metastases has increased at our centre. Although survival increased over time, the difference was not significant; this was most likely attributable to the seriousness of cases referred to our tertiary care centre. J. Surg. Oncol. 2007;96:404–410. © 2007 Wiley‐Liss, Inc.
Rapid determination of C12–C26 non‐derivatized fatty acids in human serum by fast gas chromatography
Authors: Meng Zhe , Wen Dawei , Sun Dandan , Gao Fei , Li Wei , Liao Yiping , Liu Huwei 
Published: May 2007

AbstractThe concentration of long‐chain fatty acids (LCFAs) in human serum is closely related to human health. It is therefore important to develop a fast, low‐cost, efficient method for their determination. In this study, by using fast temperature programming and micro‐bore short capillary columns, a fast gas chromatography method was developed for the direct analysis of non‐derivatized LCFAs including n‐dodecanoic acid to n‐hexacosanic acid (C12:0–C26:0, even numbers only), linoleic acid (C18:2), oleic acid (C18:1), and erucic acid (C22:1) within 4.0 min. Method optimization including extraction and separation conditions is considered, and the analysis of real serum samples is presented. The results show that ten LCFAs were well separated with sufficient resolution, and the detection limit was in the range of 2.8–9.6 μg/mL. The reproducibility (RSD) for both intra‐day and inter‐day determination was always less than 15%, and the recoveries for these LCFAs were from 63.1 to 97.0%.
Stationary phase characterization and method development
Authors: Neue Uwe D. 
Published: May 2007

AbstractThe properties of stationary phases and their characterization methods are reviewed. New and significant developments have occurred in the last few years, and new methods for stationary phase characterization have become available. The characterization methods are discussed, and the differences between the different methods are pointed out. In addition, method development approaches are reviewed, with special emphasis on recent developments that employ multiple parameters in parallel. Also, the renewed interest of temperature as a tool in method development is surveyed.
Melt Spinning of Bacterial Aliphatic Polyester Using Reactive Extrusion for Improvement of Crystallization
Authors: Vogel Roland , Tändler Bernhard , Voigt Dieter , Jehnichen Dieter , Häußler Liane , Peitzsch Lutz , Brünig Harald 
Published: May 2007

AbstractThis paper reports on an attempt to use reactive extrusion with peroxide as a comfortable pathway for improvement of the crystallization of poly(3‐hydroxybutyrate) in a melt spinning process. At first, rheological and thermal properties of the modified melts are determined in order to assess the effect of nucleation. Then spinning tests are carried out. Molecular weights and molecular weight distributions of the spun fibers are determined by chromatographic methods. Average crystallite size is measured by wide angle X‐ray scattering. Thermal and textile properties of the spun PHB fibers are also determined. An estimation of the improvement of the crystallization in the spinline and of the inhibition of the secondary crystallization in the fibers from the use of the described way of reactive extrusion is given.magnified image