bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix‐assisted laser desorption/ionization time‐of‐flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study
Authors: Chen J. , Lan P. , Tarr A. , Yan Y. M. , Francki M. , Appels R. , Ma W. 
Published: July 2007

AbstractMatrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) instrumentation has been used to analyze wheat seed gliadins as an alternative to other established methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE), capillary electrophoresis (CE), high‐performance liquid chromatography (HPLC), etc. The MALDI‐TOF approach has shown to have many advantages such as high resolution, cost effectiveness and high throughput. MALDI‐TOF‐based gliadin profiles have been used for fast wheat cultivar identification. However, the genetic information represented by individual gliadin peaks has not been utilized. In this study a wheat doubled haploid population with a genetic linkage map of good coverage was used to assay individual gliadin peaks from MALDI‐TOF profiles as molecular markers. Eight segregating peaks in the population were scored as polymorphic across the population. The 1 to 1 segregating ratios validated the scoring of the peaks and all peaks were mapped to the expected chromosomes or linkage groups on the available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 6D. Copyright © 2007 John Wiley & Sons, Ltd.
Detection of potential ion suppression for peptide analysis in nanoflow liquid chromatography/mass spectrometry
Authors: Hirabayashi Atsumu , Ishimaru Masako , Manri Naomi , Yokosuka Toshiyuki , Hanzawa Hiroko 
Published: July 2007

AbstractA detection technique for ion suppression in liquid chromatography/mass spectrometry (LC/MS) was developed by adding a probe to an LC mobile phase at a certain concentration. The probe is so hydrophilic that it is not adsorbed in a reversed‐phase nanoflow LC column, and, furthermore, has an isoelectric point of about 3, which is lower than that for most peptides and is close to the pH of the mobile phase. The intensity of the protonated probe molecule decreases much more than that of other peptides when ion suppression occurs. Thus, the occurrence of the ion suppression is detected by a decrease in the mass chromatogram for the protonated probe molecule, and the decrease ratio is higher than that for other ions. Copyright © 2007 John Wiley & Sons, Ltd.
Acoustic emission evaluation of the fracture behavior of APS‐TBCs subjecting to bondcoating oxidation
Authors: Yang L. , Zhou Y. C. , Mao W. G. , Liu Q. X. 
Published: July 2007

AbstractAir‐plasma‐sprayed (APS) thermal barrier coatings (TBCs) under the conditions of without TBC, as‐sprayed and preoxidized, were tested under tensile loading, and their acoustic emission (AE) responses were monitored. AE parameters including event count, amplitude, and frequency spectrum realized by Fast Fourier Transformation (FFT) were analyzed for information regarding the fracture behavior of TBCs. On the basis of AE behavior, the fracture process of TBCs and corresponding cracking patterns in TBCs subjected to tensile tests were evaluated. AE results also showed that the bondcoating oxidation not only promotes the cracking but also changes the failure mode in TBCs. Copyright © 2007 John Wiley & Sons, Ltd.
Interface features of ultrasonic flip chip bonding and reflow soldering in microelectronic packaging
Authors: Duan Ji‐An , Li Junhui , Han Lei , Zhong Jue 
Published: July 2007

AbstractUltrasonic features in the bonding area are of interest for researchers in the field of microelectronic packaging. In this study, the interface characteristics of bonding were examined using an XD7100 X‐ray instrument and a transmission electron microscope (TEM). It was seen that bubbles were usually detected at the interfaces of the reflow‐soldered flip chip (FC), but they were not found at the interfaces of ultrasonically formed FC, and so ultrasonic FC bonding is more reliable than reflow soldering. The strong mechanical effect of ultrasonic vibration activates the dislocations in the crystalline metal lattice. Dislocation diffusion is more prominent than crystal diffusion when the temperature is relatively low during ultrasonic bonding, and therefore the processes of ultrasonic bonding enhance by several orders of magnitude. This indicates that the mechanism of ultrasonic bonding is different from the melting mechanism of reflow soldering. Copyright © 2007 John Wiley & Sons, Ltd.
Demographic responses of an arboreal marsupial, the common brushtail possum (Trichosurus vulpecula), to a prescribed fire
Authors: Isaac Joanne L. , Valentine Leonie E. , Goodman Brett A. 
Published: July 2007

AbstractWe investigated demographic responses of the common brushtail possum Trichosurus vulpecula, a medium‐sized arboreal marsupial, after a prescribed fuel reduction burn on Magnetic Island, tropical north Queensland, Australia. Possums were live‐trapped every month for 14 months before the fire and 11 months after the fire in both the burnt and unburnt areas; measurements of individuals were taken each month and demographic parameters were modelled using capture–mark–recapture data. Significant differences between the burnt and unburnt sites were found following the fire; recruitment was lower in the unburnt area, where population size also declined. In the burnt area, population size and recruitment displayed a tendency to increase after the fire, while capture probability declined, suggesting that an influx of new individuals, attracted to re‐sprouting vegetation, had resulted in trap saturation. There was no detectable effect of the fire on survival, and no fire‐induced mortalities were observed. We conclude that a low‐intensity, prescribed, fuel‐reduction burn had no obvious negative consequences for this possum population.
The impact of specialist care for low back pain on health service utilization in primary care patients: A prospective cohort study
Authors: Chenot Jean‐François , Leonhardt Corinna , Keller Stefan , Scherer Martin , Donner‐Banzhoff Norbert , Pfingsten Michael , Basler Heinz‐Dieter , Baum Erika , Kochen Michael M. , Becker Annette 
Published: July 2007

AbstractGuidelines portray low back pain (LBP) as a benign self‐limiting disease which should be managed mainly by primary care physicians. For the German health care system we analyze which factors are associated with receiving specialist care and how this affects treatment. This is a longitudinal prospective cohort study. General practitioners recruited consecutive adult patients presenting with LBP. Data on physical function, on depression, and on utilization of health services were collected at the first consultation and at follow‐up telephone interviews for a period of 12 months. Logistic regression models were calculated to investigate predictors for specialist consultations and use of specific health care services. Large proportions (57%) of the 1342 patients were seeking additional specialist care. Although patients receiving specialist care had more often chronic LBP and a positive depression score, the association was weak. A total of 623 (46%) patients received some form of imaging, 654 (49%) physiotherapy and 417 (31%) massage. Consulting a specialist remained the strongest predictor for imaging and therapeutic interventions while disease‐related and socio‐demographic factors were less important. Our results suggest that the high use of specialist care in Germany is due to the absence of a functioning primary care gate keeping system for patient selection. The high dependence of health care service utilization on providers rather than clinical factors indicates an unsystematic and probably inadequate management of LBP.
Women's experiences following failed vaginal birth after cesarean delivery
Authors: Chigbu C.O. , Enwereji J.O. , Ikeme A.C. 
Published: July 2007

AbstractObjectiveTo assess the experience of women in whom a trial of vaginal birth after cesarean (VBAC) has failed.MethodsPretested self‐administered questionnaires containing both open‐ and closed‐ended questions were given to the participants while they were waiting for an antenatal check‐up. The responses to the closed‐ended questions were analyzed with descriptive statistics. Content analysis was used for the responses to the open‐ended questions.ResultsThe women with a history of a successful vaginal delivery reported a positive experience whereas those with no history of vaginal delivery reported a negative experience. The women found the limited options available to them the most dissatisfying aspect of attempting a VBAC.ConclusionAn unsuccessful trial of VBAC could have significant adverse effects on women, especially on those who have never given birth vaginally. Adequate antenatal education for women planning a trial of VBAC, postdelivery support following an unsuccessful trial of VBAC, and research aimed at expanding the options available to women as they are attempting VBAC are recommended.
Pediatric Head and Neck Trauma from All‐Terrain Vehicle Accidents
Authors: Wang Brian S. , Smith Stacey L. , Pereira Kevin D. 
Published: July 2007

OBJECTIVETo characterize pediatric head and neck trauma from all‐terrain vehicles (ATVs) at a single institution.STUDY DESIGN/SETTINGRetrospective case series at a level I pediatric trauma center.RESULTSThirty‐four patients were admitted: average age was 12 years, with 68% between 12 and 16 years and 32% under 12 years. The patient was the driver in 74% of cases and the passenger in 17% of cases. Driver average age was 12.7 years and passenger average age was 10.5 years. Seventy‐six percent of children were not wearing helmets. Average hospital stay was 6 days, with 81% of cases discharged home. Central nervous system injuries were most common, followed by soft tissue injuries. Soft tissue repair was the most frequent procedure, and most maxillofacial fractures required operative intervention.CONCLUSIONSATV‐related morbidity and mortality in children are significant public health issues amenable to primary prevention by prohibiting passengers on ATVs and requiring helmet use.SIGNIFICANCENational trends indicate increasing ATV‐related injuries in children.
In Vitro Discrimination of Tumor Cell Lines with an Electronic Nose
Authors: Gendron Kristin B. , Hockstein Neil G. , Thaler Erica R. , Vachani Anil , Hanson C. William 
Published: July 2007

OBJECTIVEThe electronic nose is a sensor of volatile molecules that is useful in the analysis of expired gases. Our hypothesis is that the electronic nose can distinguish between different types of upper aerodigestive tract tumor cells in vitro.STUDY DESIGNCells from both tumor and normal cell lines were suspended in saline, and a polymer composite electronic nose was used to evaluate the headspace gases. The data were subjected to principal components analysis, and Mahalanobis distances were calculated to demonstrate the ability of the electronic nose to distinguish among samples.RESULTSThe tumor cell lines, including adenocarcinoma, squamous cell carcinoma, and mesothelioma, were distinct from each other, and from the normal fibroblast and smooth muscle cells as seen on canonical discrimination plots.CONCLUSIONThe electronic nose can distinguish between tumor cell lines in vitro and has the potential to be a useful screening test for cancer.
The Inhibitory Effects of Macrolide Antibiotics on Bone Remodeling in Chronic Rhinosinusitis
Authors: Park Chan‐Soon , Park Yong‐Soo , Park Yong‐Jin , Cho Jin‐Hee , Kang Jun‐Myung , Kim Soo‐Yeon 
Published: July 2007

OBJECTIVETo investigate the effect of nasal mucosal inflammation on bone remodeling and the inhibitory effect of macrolide antibiotics on bone remodeling through the inhibition of receptor activator of nuclear factor‐kB ligand (RANKL) and macrophage colony‐stimulating factor (M‐CSF).STUDY DESIGN AND SETTINGHuman nasal fibroblasts were primary‐cultured from nasal polyp. After interleukin (IL)‐1β stimulation of fibroblasts with or without macrolide pretreatment, real‐time polymerase chain reaction for RANKL messenger RNA (mRNA) and enzyme‐linked immunosorbent assay for M‐CSF were performed at various intervals. Peripheral blood mononuclear cells (PBMCs) were cultured for 10 days with M‐CSF only, M‐CSF plus RANKL, or macrolide antibiotic plus M‐CSF and RANKL.RESULTSIL‐1β stimulation of nasal polyp fibroblasts induced expression of RANKL mRNA and secretion of M‐CSF. Macrolide antibiotics reduced RANKL mRNA and M‐CSF expression by nasal polyp fibroblasts in a dose‐dependent manner, and inhibited osteoclastogenesis from PBMCs.CONCLUSIONNasal fibroblasts stimulated with IL‐1β may take on the role of osteoblasts in osteoclastogenesis, which may be inhibited by macrolide antibiotics.
Immune Modulatory Oligonucleotides in the Prevention and Treatment of OVA‐Induced Eustachian Tube Dysfunction in Rats
Authors: Blanks Deidra A. , Ebert Charles S. , Eapen Rose P. , Zdanski Carlton , Kandimalla Ekambar R. , Agrawal Sudhir , Prazma Jiri 
Published: July 2007

BACKGROUNDOtitis media with effusion (OME) is often associated with allergies. Immune modulatory oligonucleotides (IMO) mediate allergic inflammation and may therefore be efficacious in the treatment of airway inflammation.OBJECTIVETo evaluate the role of an IMO via transtympanic mucosal application in prevention and treatment of ovalbumin‐induced OME.DESIGNForty brown Norway rats were divided into control and treatment groups. Eustachian tube dysfunction was evaluated by passive opening pressures, passive closing pressures, active clearance of negative pressure, and mucociliary clearance transit time.RESULTSRats who underwent IMO treatment required 50% less pressure to open and close the eustachian tube (P < 0.05) and were able to actively clear 50% more negative pressure than the ovalbumin‐control rats (P < 0.001). The treatment rats' mucociliary clearance time was half that of the control group (P < 0.001).CONCLUSIONIMO via transtympanic application can prevent and treat allergy‐induced eustachian tube dysfunction in rats. IMO may offer substantial promise in the future management of OME.
Utility of Head and Neck Biopsies in the Evaluation of Posttransplant Lymphoproliferative Disorder
Authors: Campisi Paolo , Allen Upton D. , Ngan Bo‐Yee , Hawkes Michael , Forte Vito 
Published: July 2007

OBJECTIVETo determine the diagnostic yield of head and neck biopsies in the evaluation of PTLD in children and to explore whether this yield was related to pretransplant Epstein‐Barr virus (EBV) serostatus.STUDY DESIGNThis is a retrospective study of pediatric, post‐solid‐organ transplant recipients who have undergone a biopsy in the head and neck region to establish a diagnosis of PTLD.RESULTSFifty‐six biopsies were performed in 46 patients four to 120 months after solid‐organ transplantation. Biopsies yielded PTLD in 39.1% of patients. The odds of developing PTLD if a patient was seropositive for EBV at the time of transplantation was 0.26 (95% confidence interval, 0.064‐1.056; P = 0.054).CONCLUSIONSThe high diagnostic yield of PTLD suggests that biopsies should be performed if PTLD is suspected in pediatric posttransplant patients. The results demonstrate a trend toward lower risk of PTLD among patients with pretransplant exposure to EBV.
Tracheotomy in the Preschool Population: Indications and Outcomes
Authors: French Lesley C. , Wootten Christopher T. , Thomas Robert G. , Neblett Wallace W. , Werkhaven Jay A. , Cofer Shelagh A. 
Published: July 2007

OBJECTIVEAlthough more tracheotomy procedures are performed within the first year of life than in any other age group, preschool‐aged children requiring tracheotomy remain understudied. We characterize the indications and outcomes for patients between the ages of 3 and 6 years undergoing tracheotomy.METHODSOut of 480 pediatric tracheotomy procedures performed at a tertiary‐care hospital between 1988 and 2004, 15 patients underwent primary tracheotomy between 3 and 6 years of age.RESULTSMost (60%) procedures were performed for pulmonary toilet. Upper‐airway obstruction represented the second most common indication (40%), and trauma necessitated tracheotomy procedures more often than had been predicted (40%). The decannulation rate was 40%; 2 patients died.CONCLUSIONTrauma contributed to both upper‐airway obstruction as well as requirements for pulmonary toilet. These procedures performed secondary to trauma will likely continue to increase.SIGNIFICANCETracheotomy procedures in the preschool population remain uncommon; however, nearly half of those studied were performed as a direct result of otherwise preventable trauma.
Stratification for Malignant External Otitis
Authors: Peleg Uri , Perez Ronen , Raveh David , Berelowitz Daniel , Cohen David 
Published: July 2007

OBJECTIVETo propose a CT‐based method for early identification of severe cases of malignant external otitis (MEO) by correlating between initial CT findings and clinical course.STUDY DESIGN AND SETTINGEighteen MEO patients who underwent CT on admission were included in this retrospective study conducted at a tertiary center. The number and extent of anatomical areas involved according to CT were compared to clinical course severity.RESULTSThe patients were categorized into two groups according to clinical course. There were 13 patients in the “nonsevere” group and 5 in the “severe.” In six out of eight CT anatomical areas the “severe” group had significantly higher scores (P< 0.05 toP< 0.0005). The average number of areas involved in the “nonsevere” group was 2.9 and in the “severe” 5.4 (P< 0.0005).CONCLUSIONWe found a clear correlation between clinical course and initial CT findings in MEO patients. Based on these findings it may be possible to predict clinical course severity according to initial CT.
Survey of Ménière's Disease in a Subspecialty Referral Practice
Authors: Vrabec Jeffrey T. , Simon Lawrence M. , Coker Newton J. 
Published: July 2007

OBJECTIVESTo define the prevalence of definite Ménière's disease (MD) among patients presenting with characteristic symptoms and examine the utility of published diagnostic guidelines.STUDY DESIGN AND SETTINGRetrospective review in an academic referral practice.RESULTSThe prevalence of definite MD in these 295 individuals was 64%. The next‐largest group (23%) consisted of patients with only cochlear symptoms. Those initially classified as probable are usually reclassified as definite with extended follow‐up. Of those with definite MD, the mean duration of disease at last follow‐up was 7.6 years, 56% were female, 19% had bilateral disease, and 34% required surgical management for vertigo.CONCLUSIONSThe 1995 AAO‐HNS guidelines are useful for classification of MD according to certainty of diagnosis and severity of disease, though some modifications could be considered.SIGNIFICANCEApplication of consistent diagnostic criteria is essential for epidemiological, genetic, or outcomes studies of Ménière's disease.
Pediatric Cartilage Interleave Tympanoplasty
Authors: Gaslin Michael , O'Reilly Robert C. , Morlet Thierry , McCormick Michael 
Published: July 2007

OBJECTIVETo describe a novel technique of cartilage tympanoplasty, and review surgical and hearing results in children.STUDY DESIGN AND SETTINGRetrospective chart review of all patients who had undergone tympanoplasty at a pediatric tertiary care hospital from August 2002 to July 2005. Forty‐two patients were identified with a minimum follow‐up time of 12 months.RESULTSMean preoperative perforation size was 21.3 percent (range 10%‐90%), and mean patient age was 7.9 years (range 3‐16 years). Median clinical follow‐up was 24 months. Tympanic membrane closure and graft integration were achieved in 40 of 42 patients (95.2%), and 35 of 42 (85.7%) patients maintained an intact, stable tympanic membrane on long‐term follow‐up. A total of 93.8 percent of patients achieved a postoperative air‐bone gap of less than or equal to 20 dB, and mean improvement in the air‐bone gap was 10.7 dB.CONCLUSION AND SIGNIFICANCECartilage interleave tympanoplasty is a versatile, stable, and effective technique for tympanic membrane repair in children.
Immune Modulatory Oligonucleotides in Prevention of Nasal Allergen—Induced Eustachian Tube Dysfunction in Rats
Authors: Ebert Charles S. , Rose Austin S. , Blanks Deidra A. , Eapen Rose P. , Kandimalla Ekambar R. , Agrawal Sudhir , Prazma Jiri , Pillsbury Harold C. 
Published: July 2007

OBJECTIVESDevelop a model of nasal allergen‐induced Eustachian tube dysfunction (ETD) in a rat and investigate the role of immune modulatory oligonucleotides (IMOs) in the prevention of nasal allergen‐induced ETD.STUDY DESIGN AND SETTINGProspective, randomized study. Brown Norway rats were sensitized to ova albumin (OVA) and randomized to receive pretreatment with IMOs or phosphate‐buffered saline. All animals were challenged intranasally with aerosolized OVA. Dynamic measures of Eustachian tube (ET) function were analyzed.RESULTSAnimals that were OVA‐sensitized and IMO‐pre‐treated had significantly lower mean passive opening (95% confidence interval [95% CI] 15.0,19.4) and closing (95% CI 4.8,7.8) ET pressures compared with those of (95% CI 24.1,32.7) and (95% CI 12.1,18,8) OVA‐sensitized untreated rats, respectively. In addition, the IMO‐pretreated animals demonstrated the ability to actively clear a significantly higher proportion of negative pressure (95% CI 0.64,0.96) compared with the untreated animals (95% CI 0.09,0.39). IMO‐pretreated animals also demonstrated significantly improved mean mucociliary clearance times in seconds (95% CI 115,195) than those in untreated animals (95% CI 308,668).CONCLUSIONSPretreatment with IMOs prevented allergen‐induced allergic inflammation around the Eustachian tube (ET) and resulted in improved ventilatory function of the ET compared with sensitized untreated animals. IMOs offer considerable promise in the management of nasal allergic disease as well as otitis media with effusion.
The Effect of Dexmedetomidine on Middle Ear Pressure
Authors: Firat Yezdan , Kizilay Ahmet , Akarcay Mustafa , Yucel Aytac , But Kadir , Yologlu Saim 
Published: July 2007

OBJECTIVEDexmedetomidine is a preferred anesthetic agent in otological surgery because it provides controlled hypotension and good surgical field visibility. The aim of this study was to evaluate the influence of this novel agent on middle ear pressure.STUDY DESIGN AND SETTINGThis prospective clinical trial was performed in 60 patients who were scheduled for elective surgery. They received dexmedetomidine or saline infusion for 20 minutes before induction of anesthesia. Tympanometric measurements were recorded for both ears at preoperative, intraoperative, and postoperative states.RESULTSMean difference of tympanometric peak pressure from baseline was statistically significant between dexmedetomidine and control group at the 30th minute of operation (24.8 daPa, P = 0.003 for right ear; 20.5 daPa, P = 0.02 for left ear) and at the end of the operation (25.8 daPa, P = 0.01 for right ear; 28.1 daPa, P = 0.004 for left ear).CONCLUSIONSDexmedetomidine anesthesia raises the tympanometric parameters, but they never exceed the limits of normal.