bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vasorin Exocytosed from Glioma Cells Facilitates Angiogenesis via VEGFR2/AKT Signaling Pathway
Authors: Zhong Ying , Kang Hui , Ma Ziqing , Li Jiayu , Qin Zixi , Zhang Zixuan , Li Peiwen , Zhong Ying , Wang Lihui 
Published: March 2024

Abstract

Glioma is a highly vascularized tumor of the central nervous system. Angiogenesis plays a predominant role in glioma progression and is considered an important therapeutic target. Our previous study showed that vasorin (VASN), a transmembrane protein, is overexpressed in glioma and promotes angiogenesis; however, the potential mechanism remains unclear. In this study, we found that human vascular endothelial cells (hEC) co-cultured with VASN-overexpressing glioma cells exhibited accelerated migration ability and increased expression of VASN originated from glioma cells. VASN was found in exosomes secreted by glioma cells and could be taken up by hECs. hECs showed more edge filopodia and significantly upregulated expression of endothelial tip cell marker gene and protein levels after co-culture with VASN-overexpressing glioma cells. In clinical glioma tissue and orthotopic transplantation glioma tissue, the vascular density and the number of vascular endothelial cells with a tip cell phenotype in VASN-overexpressed tissues were significantly higher than in tissues with low expression. At the molecular level, VASN interacted with VEGFR2 and caused internalization and autophosphorylation of VEGFR2 protein, and then activated the AKT signaling pathway. Our study collectively reveals the function and mechanism of VASN in facilitating angiogenesis in glioma, providing a new therapeutic target for glioma.

Implications:
These findings demonstrate that VASN exocytosed from glioma cells enhanced the migration of vascular endothelial cells by VEGFR2/AKT signaling pathway.
Identification of Circulating Plasma Proteins as a Mediator of Hypertension-Driven Cardiac Remodeling: A Mediation Mendelian Randomization Study
Authors: Hu Yuanlong , Lin Lin , Zhang Lei , Li Yuan , Cui Xinhai , Lu Mengkai , Zhang Zhiyuan , Guan Xiuya , Zhang Muxin , Hao Jiaqi , Wang Xiaojie , Huan Jiaming , Yang Wenqing , Li Chao , Li Yunlun 
Published: March 2024

BACKGROUND:
This study focused on circulating plasma protein profiles to identify mediators of hypertension-driven myocardial remodeling and heart failure.

METHODS:
A Mendelian randomization design was used to investigate the causal impact of systolic blood pressure (SBP), diastolic blood pressure (DBP), and pulse pressure on 82 cardiac magnetic resonance traits and heart failure risk. Mediation analyses were also conducted to identify potential plasma proteins mediating these effects.

RESULTS:

Genetically proxied higher SBP, DBP, and pulse pressure were causally associated with increased left ventricular myocardial mass and alterations in global myocardial wall thickness at end diastole. Elevated SBP and DBP were linked to increased regional myocardial radial strain of the left ventricle (basal anterior, mid, and apical walls), while higher SBP was associated with reduced circumferential strain in specific left ventricular segments (apical, mid-anteroseptal, mid-inferoseptal, and mid-inferolateral walls). Specific plasma proteins mediated the impact of blood pressure on cardiac remodeling, with FGF5 (fibroblast growth factor 5) contributing 2.96% (
P
=0.024) and 4.15% (
P
=0.046) to the total effect of SBP and DBP on myocardial wall thickness at end diastole in the apical anterior segment and leptin explaining 15.21% (
P
=0.042) and 23.24% (
P
=0.022) of the total effect of SBP and DBP on radial strain in the mid-anteroseptal segment. Additionally, FGF5 was the only mediator, explaining 4.19% (
P
=0.013) and 4.54% (
P
=0.032) of the total effect of SBP and DBP on heart failure susceptibility.

CONCLUSIONS:
This mediation Mendelian randomization study provides evidence supporting specific circulating plasma proteins as mediators of hypertension-driven cardiac remodeling and heart failure.
Iron Status and Risk of Heart Disease, Stroke, and Diabetes: A Mendelian Randomization Study in European Adults
Authors: Liu Yunan , Clarke Robert , Bennett Derrick A. , Zong Geng , Gan Wei 
Published: March 2024

Background
The relevance of iron status biomarkers for coronary artery disease (CAD), heart failure (HF), ischemic stroke (IS), and type 2 diabetes (T2D) is uncertain. We compared the observational and Mendelian randomization (MR) analyses of iron status biomarkers and hemoglobin with these diseases.

Methods and Results
Observational analyses of hemoglobin were compared with genetically predicted hemoglobin with cardiovascular diseases and diabetes in the UK Biobank. Iron biomarkers included transferrin saturation, serum iron, ferritin, and total iron binding capacity. MR analyses assessed associations with CAD (CARDIOGRAMplusC4D [Coronary Artery Disease Genome Wide Replication and Meta‐Analysis Plus The Coronary Artery Disease Genetics], n=181 522 cases), HF (HERMES [Heart Failure Molecular Epidemiology for Therapeutic Targets), n=115 150 cases), IS (GIGASTROKE, n=62 100 cases), and T2D (DIAMANTE [Diabetes Meta‐Analysis of Trans‐Ethnic Association Studies], n=80 154 cases) genome‐wide consortia. Observational analyses demonstrated J‐shaped associations of hemoglobin with CAD, HF, IS, and T2D. In contrast, MR analyses demonstrated linear positive associations of higher genetically predicted hemoglobin levels with 8% higher risk per 1 SD higher hemoglobin for CAD, 10% to 13% for diabetes, but not with IS or HF in UK Biobank. Bidirectional MR analyses confirmed the causal relevance of iron biomarkers for hemoglobin. Further MR analyses in global consortia demonstrated modest protective effects of iron biomarkers for CAD (7%–14% lower risk for 1 SD higher levels of iron biomarkers), adverse effects for T2D, but no associations with IS or HF.

Conclusions
Higher levels of iron biomarkers were protective for CAD, had adverse effects on T2D, but had no effects on IS or HF. Randomized trials are now required to assess effects of iron supplements on risk of CAD in high‐risk older people.
Intensive Blood Pressure Lowering in Individuals With Low Diastolic Blood Pressure and Elevated Troponin Levels in SPRINT
Authors: Smith Cady , Berry Jarett D. , Scherzer Rebecca , de Lemos James A. , Nambi Vijay , Ballantyne Christie M. , Kravitz Richard L. , Killeen Anthony A. , Ix Joachim H. , Shlipak Michael G. , Ascher Simon B. 
Published: March 2024

Background
Among individuals with hypertension and low diastolic blood pressure (DBP), the optimal BP target remains controversial due to concerns that BP lowering may reduce coronary perfusion. We determined the impact of intensive BP control among individuals with elevated systolic BP who have low DBP and elevated hs‐cTnT (high‐sensitivity cardiac troponin T) levels.

Methods and Results

A total of 8828 participants in SPRINT (Systolic Blood Pressure Intervention Trial) were stratified by baseline DBP. Those with low DBP (<70 mm Hg) were further stratified by elevated hs‐cTnT (≥14 ng/L) at baseline. The effects of intensive versus standard BP lowering on a cardiovascular disease composite end point, all‐cause death, and 1‐year change in hs‐cTnT were determined. The combination of low DBP/high hs‐cTnT was independently associated with a higher risk for cardiovascular disease and all‐cause death, as well as greater 1‐year increases in hs‐cTnT, compared with DBP ≥70 mm Hg. However, randomization to intensive versus standard BP lowering led to similar reductions in cardiovascular disease risk among individuals with low DBP/high hs‐cTnT (hazard ratio [HR], 0.82 [95% CI, 0.57–1.19]), low DBP/low hs‐cTnT (HR, 0.48 [95% CI, 0.29–0.79]), and DBP ≥70 mm Hg (HR, 0.73 [95% CI, 0.60–0.89];
P
for interaction=0.20). Intensive BP lowering also led to a reduction in all‐cause death that was similar across groups (
P
for interaction=0.57).

Conclusions
In this nonprespecified subgroup analysis of SPRINT, individuals with low DBP and elevated hs‐cTnT, low DBP and nonelevated hs‐cTnT, and DBP ≥70 mm Hg derived similar cardiovascular disease and mortality benefits from intensive BP lowering. These findings warrant confirmation in other studies.
Exercise‐Dependent Modulation of Immunological Response Pathways in Endurance Athletes With and Without Atrial Fibrillation
Authors: Dorian David , Gustafson Dakota , Quinn Ryan , Bentley Robert F. , Dorian Paul , Goodman Jack M. , Fish Jason E. , Connelly Kim A. 
Published: March 2024

Background
Atrial fibrillation (AF) is a common arrhythmia characterized by uncoordinated atrial electrical activity. Lone AF occurs in the absence of traditional risk factors and is frequently observed in male endurance athletes, who face a 2‐ to 5‐fold higher risk of AF compared with healthy, moderately active males. Our understanding of how endurance exercise contributes to the pathophysiology of lone AF remains limited. This study aimed to characterize the circulating protein fluctuations during high‐intensity exercise as well as explore potential biomarkers of exercise‐associated AF.

Methods and Results

A prospective cohort of 12 male endurance cyclists between the ages of 40 and 65 years, 6 of whom had a history of exercise‐associated AF, were recruited to participate using a convenience sampling method. The circulating proteome was subsequently analyzed using multiplex immunoassays and aptamer‐based proteomics before, during, and after an acute high‐intensity endurance exercise bout to assess temporality and identify potential markers of AF. The endurance exercise bout resulted in significant alterations to proteins involved in immune modulation (eg, growth/differentiation factor 15), skeletal muscle metabolism (eg, α‐actinin‐2), cell death (eg, histones), and inflammation (eg, interleukin‐6). Subjects with AF differed from those without, displaying modulation of proteins previously known to have associations with incident AF (eg, C‐reactive protein, insulin‐like growth factor‐1, and angiopoietin‐2), and also with proteins having no previous association (eg, tapasin‐related protein and α
2
‐Heremans‐Schmid glycoprotein).

Conclusions
These findings provide insights into the proteomic response to acute intense exercise, provide mechanistic insights into the pathophysiology behind AF in athletes, and identify targets for future study and validation.
Inferring Expectations from Observables: Evidence from the Housing Market
Authors: Ben-David Itzhak , Towbin Pascal , Weber Sebastian 
Published: March 2024

Abstract
We propose a method to detect shifts in housing price expectations by observing excess capacity. Anticipated future price hikes lead to increased current supply, resulting in temporary vacancies. Using a structural vector autoregression with sign restrictions, we analyze the impact of these expectations on the U.S. housing market. Our findings indicate that price expectation shocks primarily drove the 1996–2006 boom, especially in the Sand States. At the boom's peak, these shocks stemmed from unrealistic growth expectations, which reversed during the bust.
Purpose in Life: a Comparison between Communities of the Non-religious and the Religious
Authors: Robbins Mandy , Longden Gareth 
Published: March 2024

Abstract
Research has demonstrated that people of faith often report higher levels of purpose in life than the general population. It has been suggested this may be because they hold a meaningful world view rather than as a consequence of faith. It can be argued that humanists, like people of faith, also hold a meaningful world view, albeit one that is based upon a philosophy of naturalism. This article compares a sample of 1,042 members of Humanists UK with population norms, and a sample of religious groups, using the Purpose in Life Test to assess their reported levels of purpose in life. The data demonstrate that humanists record higher levels of purpose in life than the general population.
Book Publishing and Geometrical Skills in the Career of Sébastien Le Clerc
Authors: Rabinovitch Oded 
Published: March 2024

Abstract
Sébastien Le Clerc was born into a family of goldsmiths in Lorraine, and received classical artisanal training. Yet over the course of a highly successful career as an engraver, he also became a widely published scientific author. This paper argues that geometrical skills played a key role in the dual development of Le Clerc’s career, and in his striving for recognition as a man of letters, as well as an engraver. By a detailed study of the geometrical skills displayed in Le Clerc’s two geometrical publications, this paper revisits the thorny question of the relations between scholars and artisans in the early modern period. Rather than a dependence on his hands-on, bodily experience, it was Le Clerc’s skill in geometry that lent support to his aspiring scholarly career.
Checklist of Orthoptera Caelifera from Colombia
Authors: CARBONELL C. S. , ROWELL C. H.F. , BENTOS-PEREIRA A. , PORRAS M. F. 
Published: March 2024

The purpose of this list is to make known the species currently recorded from Colombia, with the aim of showing the present state of knowledge of the suborder ORTHOPTERA, CAELIFERA in this extraordinarily diverse country. The list has been derived from the unpublished Lista de Referencia de los Acridomorfos Neotropicales compiled by one of the present authors (C.S.C.), from which we have extracted the Colombian data. Other two authors have added data arising from their own work in Colombia and the fourth checked geographical names and the Von Humboldt collection.
Intrinsically Episodic Antarctic Shelf Intrusions of Circumpolar Deep Water via Canyons
Authors: Ong Ellie Q. Y. , Doddridge Edward , Constantinou Navid C. , Hogg Andrew McC. , England Matthew H. 
Published: March 2024

Abstract
The structure of the Antarctic Slope Current at the continental shelf is crucial in governing the poleward transport of warm water. Canyons on the continental slope may provide a pathway for warm water to cross the slope current and intrude onto the continental shelf underneath ice shelves, which can increase rates of ice shelf melting, leading to reduced buttressing of ice shelves, accelerating glacial flow and hence increased sea level rise. Observations and modeling studies of the Antarctic Slope Current and cross-shelf warm water intrusions are limited, particularly in the East Antarctica region. To explore this topic, an idealized configuration of the Antarctic Slope Current is developed, using an eddy-resolving isopycnal model that emulates the dynamics and topography of the East Antarctic sector. Warm water intrusions via canyons are found to occur in discrete episodes of large onshore flow induced by eddies, even in the absence of any temporal variability in external forcings, demonstrating the intrinsic nature of these intrusions to the slope current system. Canyon width is found to play a key role in modulating cross-shelf exchanges; warm water transport through narrower canyons is more irregular than transport through wider canyons. The intrinsically episodic cross-shelf transport is found to be driven by feedbacks between wind energy input and eddy generation in the Antarctic Slope Current. Improved understanding of the intrinsic variability of warm water intrusions can help guide future observational and modeling studies in the analysis of eddy impacts on Antarctic shelf circulation.
When multidisciplinary clinical practice and research meet: Quality development in the Danish Children Centers
Authors: Løkkegaard Sille Schandorph , Vang Maria Louison , Elklit Ask 
Published: March 2024

Background Collaboration between clinical practice and research is often warranted. Extended periods of collaboration integrating research and practice is however rare. This article is about a series of joint research projects through the course of 8 years involving the Danish Center of Psychotraumatology and five regional centres dedicated to combating the sequelae of child abuse across Denmark. Method We describe the development of a standardized assessment battery and how this work evolves, analyses of administrative data, what happens after the assessment, the impact of working with child abuse on employees, profiles of abuse that vary by gender, national conferences and training programs, and international collaboration. Result The collaboration between research and practice against child abuse has been seminal and is still ongoing. We have learnt about new problem areas and have produced information that can be used to serve children, employees, and civil society in better ways. Discussion This work is inspired by and aligns with initiatives abroad pertaining the construction of Children Centers, of assessments tools and child abuse trauma research. Conclusion Mutual respect, dedication, and patient persistence pave the way for significant results in a committed relationship.
Trigger factors in patients with a patent foramen ovale—associated stroke: A case-crossover study
Authors: Immens Maikel HM , Ekker Merel S , Verburgt Esmee , Verhoeven Jamie I , Schellekens Mijntje MI , Hilkens Nina A , Boot Esther M , Van Alebeek Mayte E , Brouwers Paul JAM , Arntz Renate M , Van Dijk Gert W , Gons Rob AR , Van Uden Inge WM , den Heijer Tom , de Kort Paul LM , de Laat KF , Van Norden Anouk GW , Vermeer Sarah E , Van Zagten Marian SG , Van Oostenbrugge Robert J , Wermer Marieke JH , Nederkoorn Paul J , Kerkhoff Henk , Rooyer FA , Van Rooij Frank G , Van den Wijngaard Ido R , Klijn Catharina JM , Tuladhar Anil M , ten Cate Tim JF , de Leeuw Frank-Erik 
Published: March 2024

Background: Patent foramen ovale (PFO) is a congenital anatomical variant which is associated with strokes in young adults. Contrary to vascular risk factors and atherosclerosis, a PFO is present from birth. However, it is completely unknown how an anatomical structure that is already present at birth in a large proportion of the population can convert into a PFO that causes stroke in a few. Recent studies reported a significant association between certain trigger factors and ischemic stroke in young adults. This study aims to investigate these triggers in PFO-associated stroke. Methods: The ODYSSEY study, a multicenter prospective cohort study between 2013 and 2021, included patients aged 18–49 years experiencing their first-ever ischemic event. Participants completed a questionnaire about exposure to potential trigger factors. A case-crossover design was used to assess the relative risks (RR) with 95% confidence intervals (95% CI). The primary outcome was the RR of potential trigger factors for PFO-associated stroke. Results: Overall, 1043 patients completed the questionnaire and had an ischemic stroke, of which 124 patients had a PFO-associated stroke (median age 42.1 years, 45.2% men). For patients with PFO-associated stroke, the RR was 26.0 (95% CI 8.0–128.2) for fever, 24.2 (95% CI 8.5–68.7) for flu-like disease, and 3.31 (95% CI 2.2–5.1) for vigorous exercise. Conclusion: In conclusion, flu-like disease, fever, and vigorous exercise may convert an asymptomatic PFO into a stroke-causing PFO in young adults. Data access statement: The raw and anonymized data used in this study can be made available to other researchers on request. Written proposals can be addressed to the corresponding author and will be assessed by the ODYSSEY investigators for appropriateness of use, and a data sharing agreement in accordance with Dutch regulations will be put in place before data are shared.
Natto (fermented soybeans)-induced anaphylaxis in a surfer with the possibility of sensitization to poly(γ-glutamic acid) from cutaneous exposure to jellyfish sting: a case report
Authors: Shigeno Ayami , Suzuki Tsuyoshi , Obayashi Masakazu , Asada Kei , Matsushima Satoru 
Published: March 2024

Abstract
Background
We report a case of anaphylaxis induced by natto (fermented soybeans) allergy that occurred following dermal sensitization from a jellyfish sting.

Case presentation
A 49-year-old male presented to the emergency room complaining of an acute onset of erythema with pruritis that appeared while he was surfing. Given that his heart rate dropped to ~ 40 bpm without a decline in blood pressure or oxygen saturation, we suspected anaphylaxis and administered 0.5 mg of adrenaline intramuscularly. Immediately after the muscular adrenaline injection, his heart rate recovered to ~ 60–70 bpm.

Conclusions
The major allergen that induces natto allergy is poly(γ-glutamic acid) (PGA), which is present in its mucilage. Given that PGA is also produced by jellyfish tentacles, it can be inferred that the PGA sensitization occurred via dermal exposure to jellyfish PGA. This is an example of a food allergy induced by animal stings. As PGA is a high-molecular-weight polymer, natto allergy, despite being IgE-mediated, often presents with late-onset anaphylaxis, which typically develops half a day after digestion. PGA has a wide range of applications in pharmaceuticals, cosmetics, and foods. Patients may develop allergic symptoms and experience repeated anaphylaxis with no known cause. Therefore, it is important to obtain a detailed medical history and individually instruct patients suspected of being allergic to PGA to avoid PGA-containing products.
Influence of sex on sympathetic vasomotor outflow responses to passive leg raising in young individuals
Authors: Katayama Keisho , Shiozawa Kana , Lee Jordan B. , Seo Natsuki , Kondo Haruna , Saito Mitsuru , Ishida Koji , Millar Philip J. , Banno Ryoichi , Ogoh Shigehiko 
Published: March 2024

AbstractThe purpose of this study was to clarify sex differences in the inhibition of sympathetic vasomotor outflow which is caused by the loading of cardiopulmonary baroreceptors. Ten young males and ten age-matched females participated. The participants underwent a passive leg raising (PLR) test wherein they were positioned supine (baseline, 0º), and their lower limbs were lifted passively at 10º, 20º, 30º, and 40º. Each angle lasted for 3 min. Muscle sympathetic nerve activity (MSNA) was recorded via microneurography of the left radial nerve. Baseline MSNA was lower in females compared to males. MSNA burst frequency was decreased during the PLR in both males (− 6.2 ± 0.4 bursts/min at 40º) and females (− 6.5 ± 0.4 bursts/min at 40º), but no significant difference was detected between the two groups (P = 0.61). These results suggest that sex has minimal influence on the inhibition of sympathetic vasomotor outflow during the loading of cardiopulmonary baroreceptors in young individuals.