bluuebunny/crossref_metadata_2025_split huggingface dataset:
The artificial surface‐induced whole blood inflammatory reaction revealed by increases in a series of chemokines and growth factors is largely complement dependent
Authors: Lappegård K. T. , Bergseth G. , Riesenfeld J. , Pharo A. , Magotti P. , Lambris J. D. , Mollnes T. E. 
Published: December 2007

AbstractExposing blood to an artificial surface results in a systemic inflammatory response, including cytokine release and complement activation. We studied the artificial surface‐induced inflammation in human whole blood using an extensive panel of inflammatory mediators including proinflammatory cytokines, chemokines and growth‐factors and investigated the role of the complement system in the induction of this response. Using multiplex technology, 27 different inflammatory mediators were measured after circulating blood for 4 hours in polyvinyl chloride tubing. The C3 inhibitor compstatin was used to block complement activation. A significant (p < 0.05) increase in 14 of the 27 mediators was induced by the surface, of which 7 were chemokines (IL‐8, MCP‐1, MIP‐1α, MIP‐1β, RANTES, eotaxin and IP‐10) and 5 were growth‐factors (G‐CSF, GM‐CSF, VEGF, PDGF and FGF). The traditional proinflammatory cytokines like IL‐1β, TNFα and IL‐6 were not induced, although IL‐6, as well as IL‐15 and IL‐17 increased if the surface was coated with highly bioincompatible laminaran. Inhibition of complement activation with compstatin significantly (p < 0.05) reduced the formation of 12 of the 14 mediators. For 10 of the 12 mediators, the inhibition was by 2/3 or more, for the remaining two the inhibition was more moderate. A highly biocompatible heparin‐coated PVC surface was used as negative control and completely abolished the whole inflammatory response. The artificial surface PVC markedly induced a broad spectrum of chemokines and growth‐factors, which was largely dependent on activation of complement. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Gene expression analysis of osteoblasts seeded in coral scaffold
Authors: Foo Lean Heong , Suzina A. H. , Azlina A. , Kannan T. P. 
Published: December 2007

AbstractCoral matrix of Porites sp. has the suitable properties for bone cell growth. This study was aimed to study the gene expression levels of osteoblast specific genetic markers; RUNX2, osteopontin, alkaline phosphatase and osteocalcin from osteoblasts seeded in coral scaffold, which are important in determining the feasibility of osteoblasts. Human osteoblasts were inoculated onto the processed coral in Dulbecco's Minimum Essential Medium. The cells were trypsinized on day 1, 7, 14, 18, and 21 and added with RNALater for preservation of RNA in cells. The RNA was extracted using commercial RNA extraction kit and the respective genes were amplified using RT‐PCR kit and analyzed qualitatively on 1.5% agarose gel. The expressions were evaluated with the Integrated Density Value based on the intensity of band for different periods of cell harvest. Increased expressions of the RUNX2, osteopontin, alkaline phosphatase and osteocalcin genes in the present study proved that coral is a favorable carrier for osteogenetically competent cells to attach and remain viable. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Evaluation of bone healing with eggshell‐derived bone graft substitutes in rat calvaria: A pilot study
Authors: Park Jin‐Woo , Bae Sang‐Ryul , Suh Jo‐Young , Lee Dong‐Hee , Kim Sang‐Hyun , Kim Hyungjun , Lee Chong‐Soo 
Published: December 2007

AbstractThe purpose of this study was to investigate the potential effectiveness of a surface‐modified natural calcium carbonate, hen eggshell (ES) as a bone graft substitute. The surface characteristics, cell viability on, and osteoconductivity of, particulated ES with and without hydrothermal treatment in phosphate solutions were evaluated. Hydrothermal treatment partially converted ES to calcium‐deficient hydroxyapatite (HA) with surface microstructure. MTT assay indicated higher osteoblast viability on surface‐modified ES compared with a commercially available bone substitute, anorganic bovine bone (Bio‐Oss, BO) (p < 0.001). Histological and histomorphometric analysis showed significantly greater new bone formation and mineralized bone‐to‐graft contact of surface‐modified ES, especially with hydrothermally treated ES, compared with BO in 5‐mm diameter calvarial defects in rats at 4 and 8 weeks of healing (p < 0.01). Complete bony bridging was more frequently found with hydrothermally treated ES. The results of this pilot study indicate the potential efficacy of surface‐modified particulated hen eggshell as an osteoconductive bone substitute in a rat calvarial defect model. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
The evolution of female mole ovotestes evidences high plasticity of mammalian gonad development
Authors: Carmona F. David , Motokawa Masaharu , Tokita Masayoshi , Tsuchiya Kimiyuki , Jiménez Rafael , Sánchez‐Villagra Marcelo R. 
Published: December 2007

AbstractPrevious studies of the reproductive biology and genetics of European moles (Talpa spp.) showed that all females of these species have ovotestes (gonads with testicular and ovarian tissue) instead of normal ovaries, a unique specialization among mammals. Females are fertile as their ovarian tissue is fully functional. Testicular tissue is abnormal and sterile, but produces high levels of testosterone. This phenomenon also characterizes other talpid species from Europe and North America. To study the origin of this singular reproductive specialization, we examined the gonads of several female specimens belonging to two critical taxa. Although large Japanese moles (Mogera wogura) posses ovotestes, greater Japanese shrew moles (Urotrichus talpoides) are characterized by normal ovaries. The results fit parsimoniously with a recent phylogenetic study that places Urotrichus relatively basal in the talpid tree and separate from the American shrew mole. Parsimony reconstruction on alternative phylogenetic hypotheses clearly indicates that reversal(s) must have occurred and suggests that a relatively simple genetic mechanism must be associated with the evolution of female hermaphroditism in moles. J. Exp. Zool. (Mol. Dev. Evol.) 310B:259–266, 2008. © 2007 Wiley‐Liss, Inc.
Developing strategies for detection of gene doping
Authors: Baoutina Anna , Alexander Ian E. , Rasko John E. J. , Emslie Kerry R. 
Published: December 2007

AbstractIt is feared that the use of gene transfer technology to enhance athletic performance, the practice that has received the term ‘gene doping’, may soon become a real threat to the world of sport. As recognised by the anti‐doping community, gene doping, like doping in any form, undermines principles of fair play in sport and most importantly, involves major health risks to athletes who partake in gene doping. One attraction of gene doping for such athletes and their entourage lies in the apparent difficulty of detecting its use. Since the realisation of the threat of gene doping to sport in 2001, the anti‐doping community and scientists from different disciplines concerned with potential misuse of gene therapy technologies for performance enhancement have focused extensive efforts on developing robust methods for gene doping detection which could be used by the World Anti‐Doping Agency to monitor athletes and would meet the requirements of a legally defensible test. Here we review the approaches and technologies which are being evaluated for the detection of gene doping, as well as for monitoring the efficacy of legitimate gene therapy, in relation to the detection target, the type of sample required for analysis and detection methods. We examine the accumulated knowledge on responses of the body, at both cellular and systemic levels, to gene transfer and evaluate strategies for gene doping detection based on current knowledge of gene technology, immunology, transcriptomics, proteomics, biochemistry and physiology. Copyright © 2008 John Wiley & Sons, Ltd.
Mass spectrometric study of selected precursors and degradation products of chemical warfare agents
Authors: Papoušková Barbora , Bednář Petr , Fryšová Iveta , Stýskala Jakub , Hlaváč Jan , Barták Petr , Ulrichová Jitka , Jirkovský Jaromír , Lemr Karel 
Published: December 2007

AbstractSelected precursors and degradation products of chemical warfare agents namely N,N‐dialkylaminoethane‐2‐ols, N,N‐dialkylaminoethyl‐2‐chlorides and some of related N‐quaternary salts were studied by means of electrospray ionization‐multiple tandem mass spectrometry (ESI‐MSn). Proposed structures were confirmed with accurate mass measurement. General fragmentation patterns of these compounds are discussed in detail and suggested processes are confirmed using deuterated standards. The typical processes are elimination of alkene, hydrogen chloride, or water, respectively. Besides, elimination of ethene from propyl chain under specific conditions was observed and unambiguously confirmed using exact mass measurement and labelled standard. The potential of mass spectrometry to distinguish the positional isomers occurring among the studied compounds is reviewed in detail using two different MS instruments (i.e. ion trap and hybrid quadrupole‐time of flight (Q‐TOF) analyzer). A new microcolumn liquid chromatography (µLC)/MSn method was designed for the cases where the resolution based solely on differences in fragmentation is not sufficient. Low retention of the derivatives on reversed phase (RP) was overcome by using addition of less typical ion pairing agent (1 mM/l, 3,5‐dinitrobenzoic acid) to the mobile phase (mixture water : acetonitrile). Copyright © 2007 John Wiley & Sons, Ltd.
De novo sequencing of a 21‐kDa cytochrome c4 from Thiocapsa roseopersicina by nanoelectrospray ionization ion‐trap and Fourier‐transform ion‐cyclotron resonance mass spectrometry
Authors: Branca Rui Miguel Mamede , Bodó Gabriella , Bagyinka Csaba , Prokai Laszlo 
Published: December 2007

AbstractWe have determined the primary structure of cytochrome c4 from Thiocapsa roseopersicina by de novo protein sequencing using the ‘bottom up’ approach. Three different enzymes (trypsin, endoproteinase Lys‐C, and endoproteinase Glu‐C) were employed to prepare four different sets of proteolytic digests. The digestion strategy was designed to permit a gradual buildup of smaller peptides into larger ones that were overlapped to yield the complete protein sequence. In this way we countered the main problem: peptides larger than about 1500 Da were difficult to sequence fully by tandem mass spectrometry. Direct infusion and online liquid chromatography were used on a linear ion trap Fourier‐transform ion‐cyclotron resonance hybrid instrument. The high resolving power, high mass accuracy and the availability of electron capture dissociation and collision‐induced dissociation were essential to achieve full sequence coverage. The software DeNovoX complemented by manual interpretation was used to generate sequence information from tandem mass spectra. The predominantly automated nature of data acquisition and handling allowed for a relatively straightforward and fast procedure, which could compete with the mainstream alternative of nucleotide sequence determination. Copyright © 2007 John Wiley & Sons, Ltd.
An investigation on the nature of the peptide at m/z 904, overexpressed in plasma of patients with colorectal cancer and familial adenomatous polyposis
Authors: Seraglia Roberta , Molin Laura , Tonidandel Loris , Pucciarelli Salvatore , Agostini Marco , Urso Emanuele Damiano , Bedin Chiara , Quaia Michele , Nitti Donato , Traldi Pietro 
Published: December 2007

AbstractIn an investigation devoted to the search for plasma markers for colorectal cancer (CRC), carried out by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry, a series of overexpressed peptides were identified in the plasma of patients. Among them the peptide with molecular weight 903 Da was the most abundant one, with a mean ± (SD) relative abundance of 37 ± 17% and a frequency over 60%. Interestingly, also in plasma samples of ten subjects affected by familial adenomatous polyposis (FAP), the peptide with molecular weight 903 was overexpressed. In this investigation, MALDI/MS/MS experiments were carried out on the ion at m/z 904 detected in the MALDI mass spectra of CRC and FAP patients. The data analysis by SwissProt.2007.01.09 indicates that this peptide is due to the sequence RPPGFSPF, found in the kininogen‐1 precursor, which is an α‐2‐thiol proteinase inhibitor. In the case of subjects affected by a particular FAP syndrome, the MALDI/MS/MS spectra were quite different from those obtained from CRC and FAP patients. In fact, two sequences have been evidenced: RPPGFSPF belonging to kininogen‐1 precursor, and PRKSSSSR belonging to Forkhead box protein 01A. Copyright © 2007 John Wiley & Sons, Ltd.
Development of an HPLC multi‐residue method for the determination of ten quinolones in bovine liver and porcine kidney according to the European Union Decision 2002/657/EC
Authors: Christodoulou Eleni A. , Samanidou Victoria F. , Papadoyannis Ioannis N. 
Published: December 2007

AbstractA sensitive multi‐residue analytical method was developed for the determination of ten quinolones: enoxacin, ofloxacin, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, oxolinic acid, nalidixic acid, and flumequine in bovine liver and porcine kidney. A simple liquid extraction step followed by a solid phase extraction clean up procedure was applied for the extraction of quinolones from liver and kidney tissues. Recoveries of the extraction varied between 82 and 88% for bovine liver and 92 and 95% for porcine kidney. Separation was performed on an ODS‐3 PerfectSil Target (250×4 mm) 5 μm analytical column at 25°C. The mobile phase consisted of a mixture of TFA 0.1%–CH3CN–CH3OH, delivered at a flow rate of 1.2 mL/min according to a gradient program. Elution of quinolones and the internal standard (caffeine, 7.5 ng/μL) was complete within 27 min. Photodiode array detection was used for monitoring the eluants at 275 and 255 nm. The method was fully validated according to the European Union Decision 2002/657/EC, determining linearity, selectivity, decision limit, detection capability, accuracy, and precision. The LODs of the specific method of quinolone determination in bovine liver varied between 3 and 7 μg/kg and in porcine kidney between 3 and 4 μg/kg.
Attenuation of DNA damage‐induced p53 expression by arsenic: A possible mechanism for arsenic co‐carcinogenesis
Authors: Shen Shengwen , Lee Jane , Weinfeld Michael , Le X. Chris 
Published: December 2007

AbstractInhibition of DNA repair processes has been suggested as one predominant mechanism in arsenic co‐genotoxicity. However, the underlying mode of action responsible for DNA repair inhibition by arsenic remains elusive. To further elucidate the mechanism of repair inhibition by arsenic, we examined the effect of trivalent inorganic and methylated arsenic metabolites on the repair of benzo(a)pyrene diol epoxide (BPDE)–DNA adducts in normal human primary fibroblasts and their effect on repair‐related protein expression. We observed that monomethylarsonous acid (MMAIII) was the most potent inhibitor of the DNA repair. MMAIII did not change the expression levels of some key repair proteins involved upstream of the dual incision in the global nucleotide excision repair (NER) pathway, including p48, XPC, xeroderma pigmentosum complementation group A (XPA), and p62‐TFIIH. However, it led to a marked impairment of p53 induction in response to BPDE treatment. The abrogated p53 expression translated into reduced p53 DNA‐binding activity, suggesting a possibility of downregulating downstream repair genes by p53. A p53‐null cell line failed to exhibit the inhibitory effect of MMAIII on NER, implicating a role for p53 in the NER inhibition by MMAIII. Further investigation revealed that MMAIII dramatically inhibited p53 phosphorylation at serine 15, implying that MMAIII destabilized p53 by inhibiting its phosphorylation. Because p53 is required for proficient global NER, our data suggest that arsenic inhibits NER through suppressing p53 induction in response to DNA damage in cells with normal p53 gene expression. © 2007 Wiley‐Liss, Inc.
Adaptive through‐thickness integration for accurate springback prediction
Authors: Burchitz I. A. , Meinders T. 
Published: December 2007

AbstractAccurate numerical prediction of springback in sheet metal forming is essential for the automotive industry. Numerous factors influence the accuracy of prediction of this complex phenomenon by using the finite element method. One of them is the numerical integration through the thickness of shell elements. It is known that the traditional numerical schemes are very inefficient in elastic–plastic analysis and even for simple problems they require up to 50 integration points for an accurate springback prediction. An adaptive through‐thickness integration strategy can be a good alternative. The main characteristic feature of the strategy is that it defines abscissas and weights depending on the integrand's properties and, thus, can adapt itself to improve the accuracy of integration. A concept of an adaptive through‐thickness integration strategy for shell elements is presented in this paper. Its potential is demonstrated using two examples. Calculations of a simple test—bending a beam under tension—show that for a similar set of material and process parameters the adaptive rule with seven integration points performs significantly better than the traditional trapezoidal rule with 50 points. Simulations of an unconstrained cylindrical bending problem demonstrate that the adaptive through‐thickness integration strategy for shell elements can guarantee an accurate springback prediction at minimal costs. Copyright © 2007 John Wiley & Sons, Ltd.
Transcription factor SOX‐5 enhances nasopharyngeal carcinoma progression by down‐regulating SPARC gene expression
Authors: Huang D‐Y , Lin Y‐T , Jan P‐S , Hwang Y‐C , Liang S‐T , Peng Y , Huang C‐YF , Wu H‐C , Lin C‐T 
Published: December 2007

AbstractNasopharyngeal carcinoma (NPC) is prevalent in south‐eastern Asia, and its tumourigenesis is rather complex. The purpose of this research was to identify the pivotal genes that may be altered during the early stage of NPC progression. Eleven genes were selected by comparative microarray analysis of NPC versus normal nasomucosal cells. The expression of SPARC (secreted protein, acidic, cysteine‐rich) was statistically significantly down‐regulated in NPC cells. In exploring the mechanism underlying the decreased transcription of SPARC in NPC cells, we found that the transcription factor SRY (sex‐determining region Y)‐box 5 (SOX‐5) is up‐regulated in NPC cells. RNA interference of SOX‐5 by short hairpin RNA (shRNA) in NPC cells caused a dramatic increase in SPARC and chromosome immunoprecipitation assay showed that SOX‐5 can bind directly to the SPARC promoter, suggesting that SOX‐5 acts as a key transcriptional repressor of SPARC. We further demonstrated that shRNA knockdown of SOX‐5 suppressed the proliferation of NPC cells, as well as their migratory ability, which was also observed when SPARC was over‐expressed in NPC cells. Alternatively, blocking SPARC with an antagonistic antibody reversed the effects of SOX‐5 knockdown. In 66 NPC patients, over‐expression of SOX‐5 in tumour cells correlated clinically with poor survival. Our study suggests that SOX‐5 transcriptionally down‐regulates SPARC expression and plays an important role in the regulation of NPC progression. SOX‐5 is a potential tumour marker for poor NPC prognosis. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Mössbauer studies of Fe‐Zn sulphur spinels
Authors: Bae Sung Hwan , Kim Sam Jin , Kim Chul Sung 
Published: December 2007

AbstractThe polycrystalline sample of Zn doped Fe1–xZnxCr2S4(x = 0.1, 0.3) were prepared by solid state reaction. The crystal structure was determined to be the normal cubic spinel of space group Fd 3m and the lattice constants(x = 0.1, 0.3) were a0 = 9.9967(3) Å and a0 = 9.9926(3) Å, respectively. The magnetic moment values of Fe1–xZnxCr2S4 for the x = 0.3 were observed higher than that of the x = 0.1. The Néel temperatures were decreased to 153 K (x = 0.1), 135 K (x = 0.3) with Zn substitution concentration. This result is due to the decrease of A‐B superexchange interaction by the replacement of Zn ions for A site. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Superexchange interactions in inverse spinel lithium ferrites
Authors: Hyun Sung Wook , Kim Chul Sung 
Published: December 2007

AbstractSpinel ferrites, MFe2O4 (M = Ni, Mg, Co, Li) samples were prepared by sol‐gel method. It has been studied by X‐ray diffraction, Mössbauer spectroscopy. X‐ray diffraction patterns were analyzed by the Rietveld refinement. The samples have been cubic spinel structure with the lattice constant (a0) is 8.326 ∼ 8.390 Å. The temperature dependence of the magnetic hyperfine field is analyzed by the Néel theory of ferrimagnetism. The intersublattice A‐O‐B and intrasublattice A‐O‐A superexchange interactions are found to be antiferromagnetic while the intrasublattice B‐O‐B superexchange interaction is ferromagnetic for the MFe2O4 (M = Ni, Mg, Co) samples as shown in Table 1. On the other hand, the intersublattice superexchange interaction is found to be antiferromagnetic while the intrasublattice superexchange interactions are ferromagnetic for the lithium ferrite sample. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Relation between Mössbauer spectroscopy and geometrical frustration factors in MCr1.9857Fe0.02O4 (M = Co, Zn)
Authors: Choi Kang Ryong , Kouh Taejoon , Kim Sam Jin , Kim Chul Sung 
Published: December 2007

AbstractIn order to elucidate the role of Cr ions in MCr2O4(M = Co, Zn) exhibiting geometrically frustration and multiferroic property, we have substituted a small amount of Fe ions for Cr sites and investigated the magnetic behavior of Fe ions with Mössbauer spectroscopy. The crystal structure was found to be single‐phase cubic spinel with space group of Fd3m. The lattice constants a0 and the internal structural parameter (x) of the oxygen were determined to be 8.340, 8.331 Å and 0.261 and 0.260, respectively. The Mössbauer absorption spectra at 4.2 K show that the well‐developed two sextets are superposed with small difference of hyperfine fields(Hhf). The hyperfine fields of CoCr1.9857Fe0.02O4 and ZnCr1.9857Fe0.02O4 were determined to be 490 ∼ 480 kOe and 460 ∼ 450 kOe, respectively. Isomer shift values (δ) of the two sextets are found to be 0.33 ∼ 0.35 mm/s relative to those of Fe metal, which are consistent with the high spin Fe3+ charge state. From the results of Mössbauer measurement, it is suggested that Cr3+ ions have two different magnetic sites, and there is a correlation between hyperfine fields and degree of magnetic geometrical frustration. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Magnetism of linear Fe, Co, and Ni nanowires encapsulated in zigzag (n,0) carbon nanotubes (CNT) with n = 5 to 9: A first‐principles study
Authors: Jang Y.‐R. , Lee J. I. 
Published: December 2007

AbstractWe investigated the magnetic properties of linear Fe, Co, and Ni nanowires (NW) encapsulated in zigzag (n,0) (n = 5, 6, 7, 8, and 9) carbon nanotubes (CNT) using the first‐principles plane wave calculation with the generalized gradient approximation. The magnetic moments per atom for transition metal atoms in the zigzag (n,0) with n = 8 and 9 were slightly smaller than those of freestanding transition metal nanowires with the same bond length, but the values were decreased abruptly for the NW in (6,0) and (5,0). These results suggest that the magnetic properties of linear NW in zigzag CNT are mainly determined by the size of CNT and also by the electronic property of surrounding CNT. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)