bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of Humidity and Electrostatic Charge on Anticholinergic Molecules in Dry Powder Inhalations
Authors: Yalçın Duygu Orak , Çıtak Şevval , Civan Didem , Özparlak Nur , Erdoğan Ünnügülsüm , Işık Enis , İçin Hayriye , Aldeniz Emre Erol , Kayar Gül Gönül , Dude Udaya 
Published: March 2024

Abstract

Background: Dry powder inhalers (DPIs) are commonly used for respiratory diseases such as asthma, chronic bronchitis, and chronic obstructive pulmonary disease (COPD). DPIs have many advantages for patients, such as ease of use or rapid delivery to the patient’s lungs. However, they can be very sensitive to environmental conditions such as ambient relative humidity (RH) and electrostatic charge, and therefore the manufacturing process and storage conditions of these pharmaceutical products must be carefully determined.
Method: In this study, two different active substances belonging to the anticholinergic family were studied and it was examined how the active substances of the same family might be affected by changing humidity conditions. The change in the amount of delivered active substance to the patient’s lungs as the electric charge accumulated in the capsules was determined. Additionally, the effect of electrical charge of the inhaler device on fine particle dose (FPD) was studied in capsules manufactured with Active Substance-1.
Results and Conclusion: This study demonstrated that, with the increase in ambient humidity and aging of the capsule and powder, the FPD of Active Substance-1 and the total mass increased in parallelly. While Active Substance-2 was not affected by the aging of the capsules, it was observed that the FPD value increased with the increase in ambient RH during the manufacturing process.
Bio-distribution and Toxicity Potential of Human Umbilical Cord Mesenchymal Stem Cells in Cynomolgus Monkeys
Authors: Huang Ying , Hao Xiaofang , Lin Zhi , Li Lulu , Jiang Hua , Zhang Hezhan , Geng Xingchao , Zhu Hao , Wen Hairuo 
Published: March 2024

Abstract

Mesenchymal stem cells (MSCs) have demonstrated promising advantages in the therapies of many diseases, while its multi-directional differentiation potential and immunotoxicity are the major concerns hindered their clinical translation. In this study, human umbilical Mesenchymal stem cell (hUC-MSCs) were labeled with a near-infrared fluorescent dye DiR before infused into Cynomolgus monkeys, and the amount of hUC-MSCs in the peripheral blood were dynamically estimated from 5 min to 28 days post a single administration at 3 × 106 cells/kg and 2 × 107 cells/kg intravenously. As results, some hUC-MSCs distributed to the whole body within 5 minutes, while most of the cells accumulate in the lungs along with the systemic blood circulation, and subsequently released into the blood. The toxicity potentials of hUC-MSCs were investigated in another 30 Cynomolgus monkeys, and the cells were repeatedly administrated at doses of 3 × 106 cells/kg and 2 × 107 cells/kg for 5 times on a weekly basis, with a recovery period of 1 months. hUC-MSCs showed no obvious toxic effects in cynomolgus monkeys, except xenogeneic immune rejection to human stem cells. Low level of hUC-MSCs gene at were detected in the peripheral blood of a few animals administered 2 × 107 cells/kg at 30 minutes after the first and last administration, indicating that the hUC-MSCs was not significantly amplified in vivo, indicating itssafety in non-human primate. Our study for the first time verified the safety of long-term use of hUC-MSCs in primates. We have pioneered a technology for the real-time detection of hUC-MSCs in peripheral blood and provide dynamic and rapid monitoring of the distribution characteristics of hUC-MSCs in vivo. Here, we provide data supporting the application of such products for clinical treatment and the application of stem cells in major refractory diseases and regenerative medicine.
Platelet-rich plasma treatment for large joint osteoarthritis : Retrospective study highlighting a possible treatment protocol with long-lasting stimulation of the joint with an adequate dose of platelets
Authors: Schwitzguébel Adrien , Corzo Alfredo Hernandez , Theodoridou Efstathia , ARTIERES Clémence , Roy-Camille David , Bogoev Mitko , Grange Matthieu , Boudabbous Sana , Benaim Charles 
Published: March 2024

Abstract
Platelet-rich plasma (PRP) therapy has emerged as a potential treatment option for osteoarthritis (OA) due to its ability to promote tissue healing and anti-inflammatory effects. More evidences are needed to establish the optimal therapy protocol. We present here a retrospective analysis of 252 patients treated with PRP for big joints OA between 2020 and 2022. We aimed to evaluate the benefits of PRP combined with rehabilitation on pain and function as well as the potential prognosis factors. We observed clinically significant improvements in pain (VAS improvement 49% at 6 months, 45% at 12 months) and function (Single Assessment Numeric Evaluation i.e. SANE score improvement 44% at 6 months, 39% at 12 months). Multiple PRP shoots and high sports activity, especially competition level, were found as favorable prognosis factors. The authors would suggest offering systematically PRP therapy for competition sports practitioners. Moreover, authors suggest that multiple PRP shoots, spaced from 3–4 weeks, with a total platelet count not exceeding 3*109 platlets per shoot could be a viable treatment option for OA.
Machine Learning–Based Predictive Model for Post-Stroke Dementia
Authors: Wei Zemin , Li Mengqi , Zhang Chenghui , Miao Jinli , Wang Wenmin , Fan Hong 
Published: March 2024

Abstract

Backgound: Post-stroke dementia (PSD), a common complication, diminishes rehabilitation efficacy and affects disease prognosis in stroke patients. Many factors may be related to PSD, including demographic, comorbidities, and examination characteristics. However, most existing methods are qualitative evaluations of independent factors, which ignore the interaction amongst various factors. Therefore, the purpose of this study is to explore the applicability of machine learning methods for predicting PSD.
Methods: 9 acceptable features were screened out by the Spearman correlation analysis and Boruta algorithm. We developed and evaluated 8 machine learning (ML) models: logistic regression, elastic net, k-nearest neighbors, decision tree, extreme gradient boosting, support vector machine, random forest, and multilayer perceptron.
Results: A total of 539 stroke patients were included in this study. Among the 8 models used to predict PSD, extreme gradient boosting and random forest showed the highest area under the curve (AUC), with values of 0.7287 and 0.7285, respectively. The most important features for predicting PSD included age, high sensitivity C-reactive protein, stroke side and location, and the occurrence of cerebral hemorrhage.
Conclusion: Our findings suggest that ML models, especially extreme gradient boosting, can best predict the risk of PSD.
Runx3 and Brn3a interplay orchestrates the transcriptional program in the early stages of proprioceptive neuron development
Authors: Orlovsky Kira , Appel Elena , Hantisteanu Shay , Olender Tsviya , Lotem Joseph , Levanon Ditsa , Groner Yoram 
Published: March 2024

Abstract

The development and diversification of proprioceptive neurons, which reside in the dorsal root ganglia (DRG) and express the tropomyosin receptor kinase C (TrkC), depend on the transcription factor (TF) Runx3. As one of the major functions of TrkC neurons is to coordinate limb movements, Runx3-deficient mice develop severe limb ataxia due to TrkC neuron cell death. In TrkC neurons Runx3 expression is driven by the gene proximal P2 promoter. Accordingly, P2-/- mice develop severe ataxia. Heterozygous (P2+/-) vs. homozygous (P2-/-) TrkC neuron transcriptome highlighted a repertoire of differentially expressed genes (DEG). The Intersection of Runx3 genomic occupancy-associated genes and DEG data discovered 244 high-confidence Runx3 target genes (HCT). We also analyzed the genomic occupancy of two additional TFs, Brn3a and Isl1, that play a role in sensory neuron development and determined the landscape of histone H3 acetylated lysine 27 (H3K27Ac) and transpoase-accessible chromatin with sequencing (ATAC-seq)-marked chromatin regions in E11.5 TrkC neurons. These analyses shed new light on the interplay of Runx3, Brn3a, Isl1, and open chromatin regions in regulating the HCT genes in the early developmental stages of TrkC neurons.
Large-scale network analysis of the cerebrospinal fluid proteome identifies molecular signatures of frontotemporal lobar degeneration
Authors: Saloner Rowan , Staffaroni Adam , Dammer Eric , Johnson Erik C.B. , Paolillo Emily , Wise Amy , Heuer Hilary , Forsberg Leah , Lago Argentina Lario , Webb Julia , Vogel Jacob , Santillo Alexander , Hansson Oskar , Kramer Joel , Miller Bruce , Li Jingyao , Loureiro Joseph , Sivasankaran Rajeev , Worringer Kathleen , Seyfried Nicholas , Yokoyama Jennifer , Seeley William , Spina Salvatore , Grinberg Lea , VandeVrede Lawren , Ljubenkov Peter , Bayram Ece , Bozoki Andrea , Brushaber Danielle , Considine Ciaran , Day Gregory , Dickerson Bradford , Domoto-Reilly Kimiko , Faber Kelley , Galasko Douglas , Geschwind Daniel , Ghoshal Nupur , Graff-Radford Neill , Hales Chadwick , Honig Lawrence , Hsiung Ging-Yuek , Huey Edward , Kornak John , Kremers Walter , Lapid Maria , Lee Suzee , Litvan Irene , McMillan Corey , Mendez Mario , Miyagawa Toji , Pantelyat Alexander , Pascual Belen , Paulson Henry , Petrucelli Leonard , Pressman Peter , Ramos Eliana , Rascovsky Katya , Roberson Erik , Savica Rodolfo , Snyder Allison , Sullivan A. Campbell , Tartaglia Carmela , Vandebergh Marijne , Boeve Bradley , Rosen Howie , Rojas Julio , Boxer Adam , Casaletto Kaitlin 
Published: March 2024

Abstract
The pathophysiological mechanisms driving disease progression of frontotemporal lobar degeneration (FTLD) and corresponding biomarkers are not fully understood. We leveraged aptamer-based proteomics (> 4,000 proteins) to identify dysregulated communities of co-expressed cerebrospinal fluid proteins in 116 adults carrying autosomal dominant FTLD mutations (C9orf72, GRN, MAPT) compared to 39 noncarrier controls. Network analysis identified 31 protein co-expression modules. Proteomic signatures of genetic FTLD clinical severity included increased abundance of RNA splicing (particularly in C9orf72 and GRN) and extracellular matrix (particularly in MAPT) modules, as well as decreased abundance of synaptic/neuronal and autophagy modules. The generalizability of genetic FTLD proteomic signatures was tested and confirmed in independent cohorts of 1) sporadic progressive supranuclear palsy-Richardson syndrome and 2) frontotemporal dementia spectrum syndromes. Network-based proteomics hold promise for identifying replicable molecular pathways in adults living with FTLD. ‘Hub’ proteins driving co-expression of affected modules warrant further attention as candidate biomarkers and therapeutic targets.
An Empirical Study of the Optimal Piezoelectric Patch Positioning for Voltage Generation on the Cantilever Beam
Authors: Sasikumar M 
Published: March 2024

Abstract
The ideal location for piezoelectric patches on a cantilever beam to capture electrical energy from vibration is presented in this research. Harnessing vibrations for energy generation through piezoelectric patches on cantilever beams holds great promise for powering small devices. A piezoelectric patch on a cantilever beam with a tip mass is typically used in energy harvesting devices. The majority of these gadgets employ a rectangular piezoelectric patch that covers the entire beam or just a portion of it. The position on the cantilever beam determines the voltage produced by the piezoelectric patches. The output voltage was determined experimentally in this work using a prototype harvester developed in accordance with the previously specified structure. The resultant ac output voltage was measured after the piezoelectric patches were positioned at various points along the rectangular cantilever beam. Additionally, we investigate the appearance of various solutions by varying the tip mass. Finding the best location for the energy harvester's patches and tip mass on the vibrating structure is crucial since different solutions may provide varying amounts of ac output voltage. Once this optimal position is identified, it provides a clear path to enhance the energy harvesting system's performance.
Sedentary Behaviour Associated with Frailty and Depressive Symptoms in Community- Dwelling Older Adults: a cross sectional study
Authors: Alhwoaimel Norah A , Alqahtani Bader A , Alshehri Mohammed M , Alhowimel Ahmed S 
Published: March 2024

Abstract

Background The association between the level physical activity with frailty and depressive symptoms is not yet clear in the literature. The analysis of physical activity level may provide evidence of the factors that contribute to frailty and depressive symptoms among older adults. Thus, the aim of this study was to examine the association between physical activity level (including sedentary behaviours) and both frailty and depressive symptoms in community-dwelling older adults. Methods a cross-sectional study was conducted among 428 community-dwelling older adults aged 50 and above. Physical activity level and sedentary behaviour were assessed with the Arabic version of Physical Activity Scale for the Elderly (PASE). Frailty was evaluated using the Arabic version of the FRAIL scale, and depressive symptoms was assessed using the Arabic version of the Patient Health Questionnaire 9 (PHQ-9). Descriptive statistics were used to characterize the sample. To examine the the association between physical activity level and both physical frailty and depressive symptoms, multinomial logistic regression model was used. Statistical significance was defined as p ≤ 0.05. Results The prevalence of sedentary behaviour was 24.9% (n = 106), with higher prevalence observed in frail and pre-frail individuals. Sedentary physical activity level was associated pre-frailty and frailty (OR: 2.78; 95% CI [1.47,5.25],p = 0.002), (OR: 4.58; 95% CI [1.94,10.80],p = 0.001), respectively. Further, sedentary physical activity level was significantly associated with depressive symptoms (OR: 2.42; 95% CI [1.22,4.81],p = 0.011). Conclusion Sedentary physical activity level is more prevalent among older adults who have frailty symptoms. Both frailty and depressive symptoms is significantly associated with sedentary level of physical activity. Promoting physical activity should be considered to prevent frailty and reduce depressive symptoms in older adults.
Study of the floral phenology of Dacryodes edulis in the Republic of Congo
Authors: Ossibi Grace Jokael Etou , Chrichina Mbon Nguékou , Mouekouba Dalcantara Liana Ongouya , Makoundou Alaric , Mpika Joseph , Attibayeba Paul 
Published: March 2024

Abstract
Dacryodes edulis (G. Don) HJ Lam. is a fruit tree producing edible fruits called safou. Floral phenology is described while situating it in time, in relation to temperature. And illumination. For this, 10 feet are selected in the surroundings of the city of Brazzaville. The qualitative and quantitative characters of the flowers are observed, in order to identify their phenophases. There Flowering lasts 38 days and includes three stages, the initiated flower, the flower bud and the bloomed flower which have lengths respectively 3.52 mm ; 4.742 mm and 5.569 mm. Temperature and lighting positively influence the elongation of the flower. Geographic orientation in relation to sunset and at sunrise does not influence the elongation of the flower.
High Temperature Heat Flux Sensor With ITO/In2O3 Thermopile for Extreme Environment Sensing
Authors: Tan Qiulin , Dong Helei , Lu Meimei , Wang Weifeng 
Published: March 2024

Abstract
Hypersonic vehicles and aircraft engine blades face complex and harsh environments such as high heat flow density and high temperature, and they are generally narrow curved spaces, making it impossible to actually install them for testing. Thin-film heat flux sensors(HFSs) have the advantages of small size, fast response, and in-situ fabrication, but they are prone to reach thermal equilibrium and thus fail during testing. In our manuscript, an ITO-In2O3 thick film HFS is designed and a high-temperature heat flux test system is built to simulate the working condition of a blade subjected to heat flow impact. The simulation and test results show that the test performance of the thick-film HFS is improved by optimizing the structure and parameters. Under the condition of no water cooling, the designed HFS can work under the extremely high temperature environment of 1450°C, with the maximum output thermopotential of 17.8 mV, and the average test sensitivity of 0.035 mV/(kW/m2), which has a superior high temperature resistance performance, which cannot be achieved by other existing thin (thick) film HFSs. Therefore, designed HFS has a great potential for application in harsh environments such as aerospace, weaponry, and industrial metallurgy.
RESTQ-Sport 77 Athlete Self-Report Measure for Soccer Players :The Revisit using Item Response Theory
Authors: Khusaini Nurul Syuhadah , Azidin Raja Mohammed Firhad Raja , Ramli Mohd Hanif Mohd , Aziz Norheliena , Hemdi Abdul Rahman , Mohamed Zulkifli 
Published: March 2024

Abstract

Monitoring athletes’ well-being by using subjective or objective approach is oneof the important aspects to ensure optimum performance for both technical andphysical during match or training. Athlete Self Report Measure (ASRM) is one ofthe subjective approaches and it is used widely at the global level. However, mostASRM are still printed on paper RESTQ-Sport is one of the well-known ASRM andit is established using Classical Test Theory (CTT). This study aims to re-validateRESTQ-Sport via a different approach; by implementing Item Response Theory(IRT) which will convert ordinal data to interval data for a sound and prudentobjective measurement. A total of 45 respondents volunteer to participate in thepilot study; and based on the interaction between respondents (person) and items, areliability index of 0.95 and 0.93 is recorded for both person and item. The responsefor this pilot study yields an explained variance by measures of 53.2%, while 9.2%is the unexplained variance. Further scrutiny is made for improvement, and thetest is re-analyzed. It is evident that the explained variance by measures improvetremendously to 56.9%; whilst the unexplained variance is reduced to 5.4%.
Cooperative colonization of the host and pathogen dissemination involves stochastic and spatially structured expression of virulence traits
Authors: Beuzon Carmen , Lopez-Pagan Nieves , Rufian Jose , Luneau Julien , Sanchez-Romero Maria-Antonia , Aussel Laurent , Vliet Simon van , Ruiz-Albert Javier 
Published: March 2024

Abstract
Bacteria respond to changing environments by altering gene expression. Some responses display probabilistic cell-to-cell variation within isogenic populations. A few paradigmatic examples in animal pathogens have demonstrated that this phenotypic heterogeneity has biological relevance for virulence. We investigate single-cell flagellar expression in relation to type III secretion expression in the model plant pathogen Pseudomonas syringae and describe that both systems undergo phenotypic heterogeneity throughout plant colonization. We establish that high expression of these system carries growth penalties. Stochastic, spatial and time factors shape dynamics of a phenotypically diverse population which displays division of labor during colonization: T3SSON bacteria effectors act as ‘common goods` to suppress immunity, allowing the increase of motile bacteria that actively leave the infected tissue before necrosis. This study provides a comprehensive view of how processes underlying bacterial specialization play out in the context of complex and changing environments of biological and applied relevance such as host colonization.
To check the effectiveness of transcranial direct current stimulation and mirror therapy on neonatal motor development and mental health in children with spastic quadriplegic cerebral palsy-Study protocol for randomized clinical trial
Authors: Waqas Shoaib , Ahmad Ashfaq , Goulardins Juliana Barbosa , Hanif Asif , Tariq Muhammad , Niamat Atiqa , Ali Aadil Amir 
Published: March 2024

Abstract

Background:
Cerebral palsy (CP) is a non-progressive disorder resulting from undeveloped brain function leading to delays in motor, sensory, speech, and social interaction when compared to a normal, developed child. These structural and bodily insults prompt the infant towards disability (1). Transcranial direct current stimulation (TDCS) and mirror therapy (MT) work on the concept of neuroplasity to enhance the neuromuscular development of CP children. The response of this group to the mentioned therapies has never been investigated with a large sample size, which can mimic the concept of generalizability. The goal of this study is to compare the effects of transcranial direct current stimulation and mirror therapy on neuromuscular growth and mental well-being of the spastic quadriplegic cerebral palsy population.
Methods:
This is a single-centered, double blinded, and randomized clinical trial comprised of 105 CP patients aged 3–7 years. Sampling will be done by simple random, and allocation will be done by a random number table. The assessment will be taken at baseline (Pre-I), after two weeks (Post-I), and again after ten weeks of standard of care Physical Therapy treatment (Post-II), which is routine Physical Therapy. Study variables will be age, gender, height, weight, socioeconomic status, education, and severity of symptoms. The self-constructed Shoaib motor development tool will assess motor development, motor control by the Fugal Mayer assessment tool, and muscle performance by an isokinetic dynamometer, anthropometry by scales and measuring tape, and mental health by the SDQ questionnaire.
Discussion:
Researchers anticipate that TDCS and MT will stimulate neuromuscular development in terms of motor development and influence motor control, muscle performance, anthropometric factors, and hormonal change. This, in turn, is expected to uplift the mental wellbeing of CP children under the umbrella of neuroplasity. Ultimately, the child's entire developmental curve is positively impacted, therefore improving functional capability and participation across the short-term, mid-term, and long-term activity contexts.
Registration of trial:
On January 26, 2024, the trial was registered under the reference number IRCT20231227060542N1 on the Iranian Registry of Clinical Trials (IRCT).