bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supplemental trace minerals as complexed or inorganic sources for beef cattle during the receiving period
Authors: Cheek Robin A , Kegley Elizabeth B , Russell Jason R , Reynolds Jana L , Midkiff Kirsten A , Galloway Doug , Powell Jeremy G 
Published: March 2024

Abstract
To investigate effects of inorganic or complexed trace mineral source (zinc, copper, manganese, and cobalt) on receiving period performance and morbidity, crossbred beef heifer calves (n = 287) arriving on three delivery dates were used in a 42-d receiving trial. Heifers were processed after arrival, stratified by day −1 body weights (BW) and allocated randomly to eight pens (11 to 13 heifers/pen, 24 pens total). Within truckload, pens were assigned randomly to dietary treatment (n = 12 pens/treatment). Heifers were housed on 0.42-ha grass paddocks, provided ad libitum bermudagrass hay and provided dietary treatments in grain supplements fed daily. Treatments consisted of supplemental zinc (360 mg/d), copper (125 mg/d), manganese (200 mg/d), and cobalt (12 mg/d) from complexed (Zinpro Availa 4, Zinpro Corp. Eden Prairie, MN) or inorganic sources (sulfates). Heifers were observed daily for clinical bovine respiratory disease (BRD). If presenting BRD symptoms and rectal temperature ≥ 40 °C, heifers were deemed morbid and treated with antibiotics. Six heifers/pen were bled to determine serum haptoglobin concentrations on days 0, 14, and 28. Liver biopsies were taken on day 5 ± 2 and 43 ± 1 from three calves selected randomly from each pen for mineral status comparisons. Statistical analyses were performed using the MIXED, GLIMMIX, and repeated measures procedures of SAS 9.4 with truckload as a random effect and pen within truckload specified as subject. There tended to be a treatment by day interaction for BW (P = 0.07). Heifer BW did not differ on day 0 (P = 0.82) and day 14 (P = 0.36), but heifers fed complexed trace minerals had greater BW on day 28 (P = 0.04) and day 42 (P = 0.05). Overall average daily gains were greater for heifers fed complexed trace minerals (P = 0.05; 0.78 vs. 0.70 kg, SE = 0.03). Heifers supplemented with inorganic trace minerals had greater BRD incidence (P = 0.03; 58 vs. 46%, SE = 3.6). Haptoglobin concentrations decreased throughout the trial (P &lt; 0.001), and heifers fed complexed trace minerals tended to have a decrease in haptoglobin concentrations (P = 0.07). The source of trace mineral supplementation had no effect (P ≥ 0.20) on liver mineral concentrations and there were no treatment × day interactions (P ≥ 0.35). In conclusion, supplementing diets for the first 42 d after arrival with complexed trace mineral sources improved heifer performance as compared to heifers supplemented with inorganic trace minerals.
Efficient agri-food waste valorization using mealworm (Coleoptera: Tenebrionidae) into nutrient-rich biomass for food and feed
Authors: Musembi John P , Owino Eunice A , Oyieke Florence A , Tanga Chrysantus M , Beesigamukama Dennis , Subramanian Sevgan , Cheseto Xavier , Egonyu James P 
Published: March 2024

Abstract
The utilization of yellow mealworm, Tenebrio molitor (Linnaeus, Coleoptera: Tenebrionidae), for food and feed is gaining interest globally. However, its production is hindered by expensive commercial diets. This study assessed mealworm growth performance, survival, bioconversion, and nutritional composition when fed on wheat bran (WB) with different inclusion levels (25%, 50%, 75%, and 100%) of Irish potato waste (PW). Results indicated that mealworms fed on diets with 25%–75% PW had increased body length and 1–2 times higher weight gain compared to sole WB and PW diets. The survival rate was 93%–94% across all diets. Mealworms fed on WB had a feed conversion ratio of 3.26, while the efficiency of diet conversion increased with PW inclusion levels. Mealworms fed on diets with 75% PW inclusion had the highest crude fat (48%) and energy levels (598 kcal/100 g), while sole WB produced mealworms with the highest crude protein (55%). The acid detergent fiber achieved using 100% WB was 2- to 3-fold higher, but the crude fiber and neutral detergent fiber did not vary significantly. Considerable amounts of lysine (1.6–2 mg/100 g), methionine (0.5–0.7 mg/100 g), leucine (1.4–2 mg/100 g), and threonine (0.8–1 mg/100 g) were achieved in the mealworm larvae. Our findings revealed that cheap agricultural by-products could be successfully used for the mass production of mealworms, substantially contributing to reduced production costs. Further exploration of the nutrient-dense mealworm larvae for the development of novel food and feed products is crucial.
Base-extension semantics for modal logic
Authors: Eckhardt Timo , Pym David J 
Published: March 2024

Abstract
In proof-theoretic semantics, meaning is based on inference. It may seen as the mathematical expression of the inferentialist interpretation of logic. Much recent work has focused on base-extension semantics, in which the validity of formulas is given by an inductive definition generated by provability in a ‘base’ of atomic rules. Base-extension semantics for classical and intuitionistic propositional logic have been explored by several authors. In this paper, we develop base-extension semantics for the classical propositional modal systems $K$, $KT$, $K4$ and $S4$, with $\square $ as the primary modal operator. We establish appropriate soundness and completeness theorems and establish the duality between $\square $ and a natural presentation of $\lozenge $. We also show that our semantics is in its current form not complete with respect to euclidean modal logics. Our formulation makes essential use of relational structures on bases.
CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces
Authors: Lee Yongjae , Hwang Soonkyu , Kim Woori , Kim Ji Hun , Palsson Bernhard O , Cho Byung-Kwan 
Published: March 2024

Abstract

The demand for discovering novel microbial secondary metabolites is growing to address the limitations in bioactivities such as antibacterial, antifungal, anticancer, anthelmintic, and immunosuppressive functions. Among microbes, the genus Streptomyces holds particular significance for secondary metabolite discovery. Each Streptomyces species typically encodes approximately 30 secondary metabolite biosynthetic gene clusters (smBGCs) within its genome, which are mostly uncharacterized in terms of their products and bioactivities. The development of next-generation sequencing has enabled the identification of a large number of potent smBGCs for novel secondary metabolites that are imbalanced in number compared with discovered secondary metabolites. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system has revolutionized the translation of enormous genomic potential into the discovery of secondary metabolites as the most efficient genetic engineering tool for Streptomyces. In this review, the current status of CRISPR/Cas applications in Streptomyces is summarized, with particular focus on the identification of secondary metabolite biosynthesis gene clusters and their potential applications.
This review summarizes the broad range of CRISPR/Cas applications in Streptomyces for natural product discovery and production.

One-Sentence Summary
This review summarizes the broad range of CRISPR/Cas applications in Streptomyces for natural product discovery and production.
The first living cervid species described in the 21st century and revalidation of Pudella (Artiodactyla)
Authors: Barrio Javier , Gutiérrez Eliécer E , D’Elía Guillermo 
Published: March 2024

Abstract
Several issues regarding the systematics and taxonomy of the Neotropical deer tribe Odocoileini, an assemblage of 18 recognized living species currently allocated into 7 genera, remain unclear. The few available phylogenetic analyses indicate that some genera are not monophyletic and that species richness in the group is underestimated. One genus that presents both problems are the stocky and short-legged dwarf deer, Pudu. As currently understood, it includes 2 species, the Northern pudu, Pudu mephistophiles from Peru, Ecuador, and Colombia; and the Southern pudu, P. puda, from southern Chile and nearby Argentina. Here, by means of qualitative and quantitative morphologic analysis and the assessment of genetic variation, we showed that 2 distinct species are encompassed by the current concept of P. mephistophiles. The typical form distributes north of the Huancabamba Depression from northernmost Peru to the north (Ecuador and Colombia), while the other distributes south of the Huancabamba Depression and is a Peruvian endemic. As no name is available for the last one, we describe and name it here. This is the first living cervid species described in the 21st century and the first from the New World in over 60 years. Additionally, as the Southern Pudu (the type species of Pudu) is not sister to the 2 northern pudu species, we revalidate the genus Pudella to allocate the latter 2 species.
Discovery of Trametinib as an orchestrator for cytoskeletal vimentin remodeling
Authors: Zhao Shuangshuang , Li Zhifang , Zhang Qian , Zhang Yue , Zhang Jiali , Fan Gaofeng , Cao Xiaobao , Jiu Yaming 
Published: March 2024

Abstract
The dynamic remodeling of the cytoskeletal network of vimentin intermediate filaments supports various cellular functions, including cell morphology, elasticity, migration, organelle localization, and resistance against mechanical or pathological stress. Currently available chemicals targeting vimentin predominantly induce network reorganization and shrinkage around the nucleus. Effective tools for long-term manipulation of vimentin network dispersion in living cells are still lacking, limiting in-depth studies on vimentin function and potential therapeutic applications. Here, we verified that a commercially available small molecule, trametinib, is capable of inducing spatial spreading of the cellular vimentin network without affecting its transcriptional or Translational regulation. Further evidence confirmed its low cytotoxicity and similar effects on different cell types. Importantly, Trametinib has no impact on the other two cytoskeletal systems, actin filaments and the microtubule network. Moreover, Trametinib regulates vimentin network dispersion rapidly and efficiently, with effects persisting for up to 48 h after drug withdrawal. We also ruled out the possibility that Trametinib directly affects the phosphorylation level of vimentin. In summary, we identified an unprecedented regulator Trametinib, which is capable of spreading the vimentin network toward the cell periphery, and thus complemented the existing repertoire of vimentin remodeling drugs in the field of cytoskeletal research.
Metabolomic signatures of inflammation and metabolic dysregulation in relation to colorectal cancer risk
Authors: Bever Alaina M , Hang Dong , Lee Dong Hoon , Tabung Fred K , Ugai Tomotaka , Ogino Shuji , Meyerhardt Jeffrey A , Chan Andrew T , Eliassen A Heather , Liang Liming , Stampfer Meir J , Song Mingyang 
Published: March 2024

Abstract

Background
Inflammation and metabolic dysregulation are associated with increased risk of colorectal cancer (CRC); the underlying mechanisms are not fully understood. We characterized metabolomic signatures of inflammation and metabolic dysregulation and evaluated the association of the signatures and individual metabolites with CRC risk.

Methods
Among 684 incident CRC cases and 684 age-matched controls in the Nurses’ Health Study (n = 818 women) and Health Professionals Follow-up Study (n = 550 men), we applied reduced rank and elastic net regression to 277 metabolites for markers of inflammation (C-reactive protein, interleukin 6, tumor necrosis factor receptor superfamily member 1B, and growth differentiation factor 15) or metabolic dysregulation (body mass index, waist circumference, C-peptide, and adiponectin) to derive metabolomic signatures. We evaluated the association of the signatures and individual metabolites with CRC using multivariable conditional logistic regression. All statistical tests were 2-sided.

Results
We derived a signature of 100 metabolites that explained 24% of variation in markers of inflammation and a signature of 73 metabolites that explained 27% of variation in markers of metabolic dysregulation. Among men, both signatures were associated with CRC (odds ratio [OR] = 1.34, 95% confidence interval [CI] = 1.07 to 1.68 per 1-standard deviation increase, inflammation; OR = 1.25, 95% CI = 1.00 to 1.55 metabolic dysregulation); neither signature was associated with CRC in women. A total of 11 metabolites were individually associated with CRC and biomarkers of inflammation or metabolic dysregulation among either men or women.

Conclusion
We derived metabolomic signatures and identified individual metabolites associated with inflammation, metabolic dysregulation, and CRC, highlighting several metabolites as promising candidates involved in the inflammatory and metabolic dysregulation pathways for CRC incidence.
Hyperventilation During Manual Ventilation Can Be Reduced Using a Novel Ventilator but Not With Education Interventions
Authors: Trent Andrea R , Fang Raymond , Chen Hegang , Copeland Curtis C , Roux Napoleon P , Grissom Thomas E 
Published: March 2024

ABSTRACT

Introduction
Traumatic brain injury (TBI) is the leading cause of combat casualties in modern war with an estimated 20% of casualties experiencing head injury. Since the release of the Brain Trauma Foundation’s Guidelines for the Management of Severe Traumatic Brain Injury in 1995, recommendations for management of TBI have included the avoidance of routine hyperventilation. However, both published and anecdotal data suggest that many patients with TBI are inappropriately ventilated during transport, thereby increasing the risk of morbidity and mortality from secondary brain injury.

Materials and Methods
Enlisted Air Force personnel with prior emergency medical technician training completing a 3-week trauma course were evaluated on their ability to provide manual ventilation. Participants provided manual ventilation using either an in-situ endotracheal tube (ETT) or standard face mask on a standardized simulated patient manikin with TBI on the first and last days of the course. Manual ventilation was provided via a standard manual ventilator and a novel manual ventilator designed to limit tidal volume (VT) and respiratory rate (RR). Participants were given didactic and hands-on training on the third day of the course. Half of the participants were given simulator feedback during the hands-on training. All students provided 2 minutes of manual ventilation with each respirator. Data were collected on the breath-to-breath RR, VT, and peak airway pressures generated by the participant for each trial and were averaged for each trial. A minute ventilation (MV) was then derived from the calculated RR and VT.

Results
One hundred fifty-six personnel in the trauma course were evaluated in this study. Significant differences were found in the participant’s performance with manual ventilation with the novel compared to the traditional ventilator. Before training, MV with the novel ventilator was less than with the traditional ventilator by 2.1 ± 0.4 L/min (P = .0003) and 1.6 ± 0.5 L/min (P = .0489) via ETT and face mask, respectively. This effect persisted after training with a difference between the devices of 1.8 ± 0.4 L/min (P = .0069) via ETT. Both traditional education interventions (didactics with hands-on training) and simulator-based feedback did not make a significant difference in participant’s performance in delivering MV.

Conclusions
The use of a novel ventilator that limits RR and VT may be useful in preventing hyperventilation in TBI patients. Didactic education and simulator-based feedback training may not have significant impact on improving ventilation practices in prehospital providers.
Bronchoalveolar Lavage Fluid Cytology of Deployed Military Personnel With Chronic Respiratory Symptoms From the STAMPEDE III Study
Authors: Boster Joshua M , Moore III William J , Stoffel Steven T , Anderson Jess T , Gonzales Michael A , Houle Mateo C , Walter Robert J , Morris Michael J 
Published: March 2024

ABSTRACT

Introduction
Deployed military personnel may be at risk for developing acute and chronic lung disease. Prior studies of this patient population have revealed that unexplained exertional dyspnea is the most common diagnosis despite an extensive evaluation. There is a concern that an occult disorder may be affecting this population. This study evaluated the role for bronchoalveolar lavage (BAL) fluid analysis in the evaluation of chronic deployment-associated dyspnea.

Materials and Methods
Military personnel who reported chronic respiratory symptoms were evaluated as part of the Study of Active Duty Military for Pulmonary Disease Related to Environmental Deployment Exposures III study. Participants underwent bronchoscopy with BAL as part of a standardized evaluation.

Results
A total of 308 patients with a mean age of 38 ± 8.6 years underwent bronchoscopy with BAL. BAL cell-count percentages of macrophages, lymphocytes, neutrophils, and eosinophils were: 76.2 ± 17.0%, 16.3 ± 13.4%, 6.6 ± 8.9%, and 0.9 ± 3.2%, respectively. There was no clear differentiation between groups based on increases in lymphocyte counts (P = .640), although lymphocyte values were more elevated (21.4 ± 12.1%) in the interstitial lung disease category. Neutrophil counts (6.6 ± 8.9%) were elevated compared to the reported normal reference values and were increased in the isolated pulmonary function test abnormality (9.4 ± 11.6%), large airway disorder (10.0 ± 7.5%), miscellaneous (10.9 ± 20.2%), and obstructive lung disease (11.0 ± 15.6%) groups. Eosinophil counts were within normal limits (0.9 ± 3.2%) and showed no differences between groups (P = .545); asthma patients trended higher (1.6 ± 5.7%). BAL counts for the exertional dyspnea group were within normal reference values and showed no differences from the entire cohort.

Conclusions
The addition of BAL cytology did not help differentiate those patients with unexplained dyspnea from other etiologies.
Stellar feedback impact on the ionized gas kinematics in the dwarf galaxy Sextans B
Authors: Gerasimov Ivan S , Egorov Oleg V , Moiseev Alexei V , Kniazev Alexei Yu , Lozinskaya Tatiana A , Egorova Evgeniya S 
Published: March 2024

ABSTRACT
We investigated the ionized and atomic gas kinematics and excitation state in the central region of ongoing star formation of the nearby low-metallicity dwarf galaxy Sextans B. The analysis is based on the new observations performed in the H α emission line with high resolution (R ∼ 16 000) scanning Fabry–Perot interferometer at the 6-m BTA Special Astrophysical Observatory of the Russian Academy of Sciences telescope, and on the long-slit spectral observations at the 9.2-m SALT and 2.5-m Caucasian Mountain Observatory of Sternberg Astronomical Institute of Moscow State University telescopes. Strong non-circular gas motions detected in the studied regions probably resulted from the off-plane gas motions and impact of stellar feedback. We identified six regions of elevated H α velocity dispersion, five of which exhibit asymmetric or two-component H α line profiles. Three of these regions are young (&lt;1.1 Myr) expanding ($V_\mathrm{exp} \sim 25-50 \, \mbox{km}\, \mbox{s}^{-1}$) superbubbles. We argue that at least three regions in the galaxy could be supernova remnants. We conclude that supernovae feedback is the dominant source of energy for superbubbles in Sextans B, which is expected for such a low metallicity, although we cannot rule out a strong impact of pre-supernova feedback for one superbubble.
Swift monitoring of GK Persei during the 2018 dwarf nova outburst
Authors: Pei Songpeng , Orio Marina , Zhang Xiaowan 
Published: March 2024

ABSTRACT
The old nova and intermediate polar (IP) GK Persei underwent one of its recurrent dwarf nova (DN) outbursts in 2018. We proposed monitoring it in UV and X-rays with the Neil Gehrels Swift Observatory, starting less than six days after the eruption, until 16 days after the eruption ended. For the first time, we could follow the decay to the minimum light UV and X-rays. We present the timing and spectral analysis, comparing the results with the previous outbursts and with the quiescent status. We confirm the spin modulation in X-rays with a period PWD = 351.325(9) s, only in the 2–10 keV range. The period was not detected in the 0.3–2 keV range and in the UV band, suggesting that the soft portion of the X-ray spectrum in GK Per does not originate near the poles, but in a wind or circumstellar material. The amplitude of the modulation was less prominent than in 2015, a fact that seems correlated with a lower average mass accretion rate. The spectral fits are consistent with a mass accretion rate increasing by a factor of 2 from rise to maximum and decreasing during the return to minimum, following the trend of the modulation amplitude. The maximum plasma temperature is higher than the Swift XRT energy range of 0.3–10 keV, thus it is not well constrained, but our spectral fits indicate that it may have varied irregularly during the outburst.
Evaluation of medication withdrawal in patients with non-systemic juvenile idiopathic arthritis in Japan using a web-based survey
Authors: Ebato Takasuke , Kishi Takayuki , Akamine Keiji , Nozawa Tomo , Imagawa Tomoyuki , Bando Yuki , Miyamae Takako 
Published: March 2024

ABSTRACT

Objectives
Although treatments for juvenile idiopathic arthritis (JIA) have seen considerable advancements, there remains a lack of clear guidelines on withdrawing medications. This study aimed to investigate the current strategies for discontinuing non-systemic JIA treatment.

Methods
A web-based questionnaire was distributed to members of the Pediatric Rheumatology Association of Japan.

Results
According to 126 responses, the most significant factors influencing JIA treatment tapering were the duration of clinically inactive disease, medication toxicity, and a history of arthritis flares. Respondents were often cautious about discontinuing medication if symptoms, e.g. ‘morning stiffness’ or ‘intermittent joint pain’, persisted. Among subtypes, oligoarticular JIA was more amenable to treatment tapering, whereas rheumatoid factor–positive polyarticular JIA proved less amenable. Most respondents started medication tapering after a continuous clinical inactive duration exceeding 12 months, and &gt;50% of them required &gt;6 months to achieve treatment discontinuation. Additionally, 40% of the respondents consistently underwent imaging before treatment tapering.

Conclusions
The relative risks of treatment continuation and withdrawal should be considered, and decisions should be made accordingly. To obtain improved understanding of and more robust evidence for the optimal strategies for safely discontinuing JIA treatment, it is crucial to continue investigations including long-term outcomes.
Successful treatment of lupus protein-losing enteropathy with belimumab: A case report
Authors: Kojima Marenori , Hanaoka Hironari , Aoki Kaito , Matsushita Hiromi , Ito Hiroshi , Yamada Hidehiro 
Published: March 2024

ABSTRACT
Lupus protein-losing enteropathy (LUPLE) is a rare condition in patients with systemic lupus erythematosus (SLE). Since the causes and exact pathological mechanism have not been elucidated, appropriate treatment has not been determined. Here, we report the case of a 69-year-old woman with systemic lupus erythematosus who developed LUPLE which was successfully treated with belimumab without an increase in glucocorticoid dose. This case suggests that belimumab monotherapy may be a treatment option for LUPLE.