bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacodynamics and biodistribution of [195mPt]cisplatin(CISSPECT®) in head and neck squamous cell carcinoma
Authors: de Roest Reinout H. , Stigter van Walsum Marijke , van der Schilden Karlijn , Brakenhoff Ruud H. 
Published: March 2024

Abstract
Background
Cisplatin- based chemoradiotherapy is a crucial pillar in the treatment of HNSCC. The use of cisplatin comes with high toxicity rates as 35% of patients cannot sustain the planned dose while response is unpredictable. Unfortunately, there are no clinically applicable biomarkers to predict response. Based on the association of response with the number of DNA adducts and the involved molecular pathway to resolve cisplatin-induced DNA crosslinks in HNSCC, [195mPt]cisplatin (CISSPECT®) might have potential to monitor drug uptake and retention before treatment, and predict cisplatin response. The aim of this study is to investigate this concept by analyzing uptake, retention and biodistribution of [195mPt]cisplatin between known cisplatin-sensitive (VU-SCC-1131) and –resistant (VU-SCC-OE) HNSCC cell lines in vitro and xenografted in mice in vivo.

Results
By a variety of experiments in vitro, including cell cycle analyses, and in vivo, the sensitivity of cell line VU-SCC-1131 and resistance of cell line VU-SCC-OE for cisplatin was demonstrated. VU-SCC-OE was able to accumulate more [195mPt]cisplatin in the DNA, and showed an increased capability to repair [195mPt]cisplatin crosslinks compared to VU-SCC-1131. Notably, DNA binding of cisplatin increased even when cisplatin was removed from the medium, likely from intracellular sources. In vivo, [195mPt]cisplatin showed a rapid biodistribution to the large organs such as the liver, with no differences between intravenous and intraperitoneal administration. Most circulating [195mPt]cisplatin was cleared by renal filtration, and accumulation in kidney and liver remained high. Uptake in xenografts was rapid (blood:tumor ratio; 1:1) and highest after 1 h, while decreasing after 6 h in line with the concentration in the blood. Remarkably, there was no significant difference in uptake or retention between xenografts of the cisplatin-sensitive and -resistant cell line.

Conclusion
VU-SCC-1131 with a known FA deficiency and VU-SCC-OE displayed a significant difference in sensitivity to and recovery from cisplatin treatment, due to S-phase problems in VU-SCC-1131 at low doses, in line with the genetic defect. Using Pt-195m radioactivity analysis, we demonstrated the limited capability of cisplatin crosslink repair in VU-SCC-1131. Unexpectedly, we were not able to translate these findings to a mouse model for sensitivity prediction based on the biodistribution in the tumor, most likely as other factors such as influx counterbalanced repair. These data do not support response prediction by [195mPt]cisplatin, and applications to predict the toxic side-effects of cisplatin and to tailor dosing schemes seem more feasible.
End-of-life expenditure on health care for the older population: a scoping review
Authors: Kocot Ewa , Ferrero Azzurra , Shrestha Shibu , Dubas-Jakóbczyk Katarzyna 
Published: March 2024

Abstract
Background
The existing evidence shows that the pattern of health expenditure differs considerably between people at the end-of-life and people in other periods of their lives. The awareness of these differences, combined with a detailed analysis of future mortality rates is one of the key pieces of information needed for health spending prognoses. The general objective of this review was to identify and map the existing empirical evidence on end-of-life expenditure related to health care for the older population.

Methods
To achieve the objective of the study a systematic scoping review was performed. There were 61 studies included in the analysis. The project has been registered through the Open Science Framework.

Results
The included studies cover different kinds of expenditure in terms of payers, providers and types of services, although most of them include analyses of hospital spending and nearly 60% of analyses were conducted for insurance expenditure. The studies provide very different results, which are difficult to compare. However, all of the studies analyzing expenditure by survivorship status indicate that expenditure on decedents is higher than on survivors. Many studies indicate a strong relationship between health expenditure and proximity to death and indicate that proximity to death is a more important determinant of health expenditure than age per se. Drawing conclusions on the relationship between end-of-life expenditure and socio-economic status would be possible only by placing the analysis in a broader context, including the rules of a health system’s organization and financing. This review showed that a lot of studies are focused on limited types of care, settings, and payers, showing only a partial picture of health and social care systems in the context of end-of-life expenditure for the older population.

Conclusion
The results of studies on end-of-life expenditure for the older population conducted so far are largely inconsistent. The review showed a great variety of problems appearing in the area of end-of-life expenditure analysis, related to methodology, data availability, and the comparability of results. Further research is needed to improve the methods of analyses, as well as to develop some analysis standards to enhance research quality and comparability.
Ultrasound findings and specific intrinsic blood volume expansion therapy for neonatal capillary leak syndrome: report from a multicenter prospective self-control study
Authors: Liu Jing , Gao Yue-Qiao , Fu Wei 
Published: March 2024

Abstract
Objective
Capillary leak syndrome (CLS) is characterized by severe systemic edema without specific treatment, resulting in a high mortality rate. This study investigated whether there is organ edema in neonatal CLS patients and specific treatment strategies to improve patient prognosis.

Methods
Thirty-seven newborns diagnosed with CLS were included in this study. (1) Routine point-of-care ultrasound (POCUS) was used to identify whether the patients had visceral edema or fluid collection. (2) All patients were treated with 3% NaCl intravenously, and the clinical manifestations, laboratory indices and outcomes were compared before and after treatment.

Results
(1) Diffuse severe edema was found in 92.0% of the patients. (2) The POCUS examination revealed that CLS patients exhibited significant visceral edema in addition to diffuse severe edema, which included pulmonary edema in 67.6%, cerebral edema in 37.8%, severe intestinal edema in 24.3%, severe myocardial edema in 8.1%, pericardial effusion in 5.4%, pleural effusion in 29.7% and peritoneal effusion in 18.9%. Two patients (5.45%) had only myocardial edema without other manifestations. (3) Before and after the intravenous injection of 3% NaCl, there were no significant differences in the serum sodium or potassium levels of CLS patients, while the hemoglobin and hematocrit levels were significantly lower after treatment (p < 0.01). Her plasma ALB concentration and arterial pressure returned to normal levels after the treatment was completed. (4) All the patients survived, and no side effects or complications were observed during or after treatment with 3% NaCl.

Conclusions
(1) In addition to diffuse severe edema, visceral edema and effusion are common and important clinical manifestations of neonatal CLS and need to be detected by routine POCUS. (2) The intravenous injection of 3% NaCl is a safe, effective and specific treatment strategy for neonatal CLS, with a survival rate of 100% and no adverse effects.
tRNAGlu-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching
Authors: Fan Yuan , Pavani Krishna Chaitanya , Smits Katrien , Van Soom Ann , Peelman Luc 
Published: March 2024

AbstractTransfer RNA-derived small RNAs (tsRNAs) have been shown to be involved in early embryo development and repression of endogenous retroelements in embryos and stem cells. However, it is unknown whether tsRNAs also regulate embryo hatching. In this study, we mined the sequencing data of a previous experiment in which we demonstrated that the microRNA (miRNA) cargo of preimplantation embryonic extracellular vesicles (EVs) influences embryo development. We thus profiled the tsRNA cargo of EVs secreted by blastocysts and non-blastocysts. The majority of tsRNAs was identified as tRNA halves originating from the 5´ ends of tRNAs. Among the 148 differentially expressed tsRNAs, the 19 nt tRNA fragment (tRF) tDR-14:32-Glu-CTC-1 was found to be significantly up-regulated in EVs derived from non-blastocysts. RT-qPCR assays confirmed its significant up-regulation in non-blastocyst embryos and their conditioned medium compared to the blastocyst group (P < 0.05). Inhibition of tDR-14:32-Glu-CTC-1 by supplementing antagomirs to the conditioned medium improved embryo hatching (P < 0.05). Transcriptomic analysis of embryos treated with tDR-14:32-Glu-CTC-1 antagomirs further showed differential expression of genes that are associated with embryo hatching and implantation. In summary, tDR-14:32-Glu-CTC-1 is up-regulated in non-blastocyst embryos and their secretions, and inhibition of tDR-14:32-Glu-CTC-1 promotes embryo hatching, while influencing embryo implantation-related genes and pathways. These results indicate that embryonic EVs containing specific tRFs may regulate preimplantation embryo development.
Mechanism for formation of porcine blood hydrogels used as additives in the mortar of traditional Chinese architectural painting
Authors: Cheng Cong , Zhu Yeting , Zhang Jingyi , Li Wei , Teri Gele , Zheng Lizhen , Hu Daodao 
Published: March 2024

Abstract
Ancient Chinese architecture drawing is a unique form of ancient Chinese art. Porcine blood hydrogels are distinctive ingredients used to prepare the composite material of the substrate layer for architecture drawing. This investigation was focused on the mechanism for porcine blood hydrogel formation. Based on the traditional Chinese recipe for the preparation of porcine blood-lime mortar, samples with different ratios of porcine blood and lime water were prepared, and the molecular-scale interactions between the lime water and proteins in the porcine blood were examined with FTIR spectroscopy, Raman spectroscopy, XRD, XPS, fluorescence spectroscopy, contact angle and rheology studies. The mechanical properties and morphological features of the samples were detected with a rheometer, universal material testing machine and SEM. The results indicated that an appropriate amount of lime water was required for gelation of the porcine blood. With the appropriate alkalinity, sufficient carboxyl groups on the fibrin chains were deprotonated, and coordination with Ca2+ ions formed enough cross-links for support networks within the structure of the porcine blood hydrogel. Complexation of deprotonated carboxyl groups on the fibrin chains with Ca2+ ions led to the formation of a hydrophobic surface due alterations of the fibrin conformation and increased the adhesive properties of the hydrogel. With the hydrogel used as an ingredient in the preparation of a substrate layer composite, it is suggested that the hydrophobic surface of the hydrogel facilitated mixing with hydrophobic tung oil during preparation of the composite material, and strong adhesion of the hydrogel increased the mechanical strength and crack resistance of the substrate layer.
Deep learning-based metastasis detection in patients with lung cancer to enhance reproducibility and reduce workload in brain metastasis screening with MRI: a multi-center study
Authors: Park Yae Won , Park Ji Eun , Ahn Sung Soo , Han Kyunghwa , Kim NakYoung , Oh Joo Young , Lee Da Hyun , Won So Yeon , Shin Ilah , Kim Ho Sung , Lee Seung-Koo 
Published: March 2024

Abstract
Objectives
To assess whether a deep learning-based system (DLS) with black-blood imaging for brain metastasis (BM) improves the diagnostic workflow in a multi-center setting.

Materials and methods
In this retrospective study, a DLS was developed in 101 patients and validated on 264 consecutive patients (with lung cancer) having newly developed BM from two tertiary university hospitals, which performed black-blood imaging between January 2020 and April 2021. Four neuroradiologists independently evaluated BM either with segmented masks and BM counts provided (with DLS) or not provided (without DLS) on a clinical trial imaging management system (CTIMS). To assess reading reproducibility, BM count agreement between the readers and the reference standard were calculated using limits of agreement (LoA). Readers’ workload was assessed with reading time, which was automatically measured on CTIMS, and were compared between with and without DLS using linear mixed models considering the imaging center.

Results
In the validation cohort, the detection sensitivity and positive predictive value of the DLS were 90.2% (95% confidence interval [CI]: 88.1–92.2) and 88.2% (95% CI: 85.7–90.4), respectively. The difference between the readers and the reference counts was larger without DLS (LoA: −0.281, 95% CI: −2.888, 2.325) than with DLS (LoA: −0.163, 95% CI: −2.692, 2.367). The reading time was reduced from mean 66.9 s (interquartile range: 43.2–90.6) to 57.3 s (interquartile range: 33.6–81.0) (P <.001) in the with DLS group, regardless of the imaging center.

Conclusion
Deep learning-based BM detection and counting with black-blood imaging improved reproducibility and reduced reading time, on multi-center validation.
Lay beliefs of COVID-19 vaccine refusal among intercity commercial drivers in the Volta region of Ghana: recommendations for improved vaccine uptake
Authors: Manu Emmanuel , Douglas Mbuyiselo , Kushitor Mawuli Komla , Komesuor Joyce , Ampomah Mary Akua , Opoku Nicholas Obuobisa 
Published: March 2024

Abstract
Background
The COVID-19 vaccine has faced increased hesitancy in Ghana and the Volta region in particular since its rollout. Acceptance of the vaccine among intercity commercial drivers is crucial, especially in the Volta region, as they transport people within and outside the country and could fuel the transmission of the virus if not vaccinated.

Objective
We therefore established lay beliefs surrounding COVID-19 vaccine refusal among intercity commercial drivers in the Volta region of Ghana, as well as their recommendations for improved vaccine uptake.

Methods
We purposively interviewed twenty-five (25) intercity commercial drivers who had not been vaccinated for COVID-19 in the Volta region of Ghana using a semi-structured interview guide and analysed their responses thematically using the ATLAS.ti software.

Results
Various (ten) beliefs surrounding COVID-19 vaccine refusal were identified. These include the nonexistence of COVID-19, being immune to COVID-19, and the belief in the nonexistence of vaccines and vaccines being meant for the sick. Other beliefs identified were the belief that the COVID-19 vaccine is meant to reduce Africa’s population, that the vaccine triggers other health complications leading to death, the belief that vaccination could cause financial loss, political mistrust, that the COVID-19 vaccine is not permitted by God, and the belief that prayer prevents COVID-19 infection. They also suggested that the adoption of persuasive communication techniques, the publication of information on those who died of COVID-19, providing evidence of tests conducted on the vaccine, testing people before vaccination, provision of care to those who may experience side effects from the vaccine, and being able to explain why varied vaccines are used for the same virus could help improve vaccine uptake.

Conclusion
Our findings show that there is a general lack of understanding and mistrust surrounding the COVID-19 vaccine among intercity commercial drivers in the Volta region. Hence, health promotion officers and communicators in the region need to be knowledgeable on the vaccine as well as on the conspiracy theories thwarting its uptake to provide comprehensive education to the public and intercity commercial drivers to improve its uptake.
Patients’ experience of a novel interdisciplinary nurse-led self-management intervention (INSELMA)—a qualitative evaluation
Authors: Blum Nadine Schäffer , Esbensen Bente Appel , Østergaard Mikkel , Bremander Ann , Hendricks Oliver , Lindgren Luise Holberg , Andersen Lena , Jensen Kim Vilbaek , Primdahl Jette 
Published: March 2024

Abstract
Background
Despite continuous improvements in anti-rheumatic pharmacological treatment, people with chronic inflammatory arthritis still report substantial disease impact. Based on the framework for complex interventions, we thus developed INSELMA, a novel nurse-coordinated multidisciplinary self-management intervention for patients with rheumatoid arthritis, psoriatic arthritis or axial spondyloarthritis. Based on individual biopsychosocial assessments, a rheumatology nurse facilitated goal setting and coordinated interdisciplinary support. The aim of this study was to explore the patients’ experience of participating in the six-months INSELMA intervention.

Methods
Individual semi-structured interviews were conducted with 15 of the participants after their final follow-up. Thematic analysis was applied.

Results
The analysis derived four overall themes. (1) A new opportunity at the right time. The participants’ disease impacted all areas of daily life. Participation in INSELMA was experienced as an opportunity to improve symptoms and together reduce long-held challenges they had fought alone, until now. (2) The importance of person-centred goals. The participants found it meaningful to work with their individual goals, which encompassed physical, psychological, and social factors. Having time between consultations to work with goals at home was important. (3) Empathy, partnership and a little nudging from health professionals are essential. The empathic nurses’ continuous support and coaching helped participants become aware of their own resources. The participants highlighted having access to support from a physiotherapist and occupational therapist with rheumatology experience as important. (4) I got more than I could have hoped for. Most of the participants experienced decreased symptom load and improvement in physical strength, mobility, sleep, and mood as well as increased energy, knowledge, and self-management ability. The participants expressed new hope for the future with an improved ability to manage their symptoms and work towards new goals.

Conclusion
The participants found the INSELMA intervention meaningful and feasible. They experienced decreased disease impact and increased activity levels, facilitated by empathy and self-management support from health professionals.
Strengthening partnerships for the Sustainable Development Goals: engaging faith-based organizations and scientists in youth climate change learning and action
Authors: Boorse Dorothy F. , Jablonski Leanne M. 
Published: March 2024

AbstractCollaborations with and among faith-based organizations (FBOs), natural scientists, development organizations, and youth offer untapped potential for achieving the United Nations Sustainable Development Goals (SDGs). FBOs are important cultural partners for achieving the SDGs globally in numbers of adherents (80% of humanity) and resources, owning 8% of habitable land, half the world’s schools, and many health and community development services; they provide assets for formal and informal education, forming youth leaders, and social justice action. Natural scientists are also critical to SDG achievement because the environmental components in many goals require specialized skills and background. We focus on Climate Action (SDG 13) as it links to other SDGs and because climate justice directly connects to human development - a major area of FBO work, and engages scientist outreach and youth action. We surveyed The UN Environmental Program (UNEP) Faith for Action and other major FBO networking initiatives that were formed to address the SDGs as well as literature covering other interfaces and partnerships between religion, sustainability, youth, SDGs, science, climate change, and education. We examined curriculum and guidebooks to identify best practices and identified gaps in partnership development. The Faith Pavilion and multifaith statements at COP28 and the international Catholic movements emerging from the SDGs-concurrent 2015 Laudato Si' encyclical and its follow-up 2023 Laudate Deum are exemplary in mobilizing educators, institutions, and organizations to integrate SDGs into activities through a faith and particular mission lens. International development FBOs and diverse religious groups worldwide support climate adaptation and mitigation by connecting their central mission to environmental care. Today’s youth are unprecedented in number, sense of urgency, experience of climate anxiety and grief, and engaged sustainability actions. Yet, youth, FBOs and scientists are often not included in SDGs work. We recommend developing partnership skills, cultural and religious literacy materials, programs, and toolkits for secular groups and scientists partnering with FBOs, and networking opportunities to form partnerships. Connecting FBOs, including youth organizations, to larger youth climate movements and other collaborative climate networks, and creating climate action materials connecting to faith backgrounds of particular youth, will better equip potential partners for achieving the SDGs.
Energy optimization of stand-alone electrical grid considering the optimal performance of the hydrogen storage system and consumers
Authors: Peng Yizhao , Zhang Yingmin 
Published: March 2024

AbstractThe surge in energy storage systems and the increasing involvement of demand-side participation can be attributed to their favorable characteristics, including their seamless integration into electrical networks and their capacity to offer operational flexibility during critical periods. This scholarly article focuses on enhancing energy utilization in an autonomous electrical grid by incorporating hydrogen storage and demand-side participation. The optimization of the stand-alone electrical grid is based on maximizing efficiency and minimizing energy consumption costs as the main objective functions are modeled. The modeling efficiency is formulated considering the ratio of the energy not supplied (ENS) to energy generation by resources. And costs of energy consumption are modeled as consumption of fuel costs by resources. The consumers’ participation is proposed based on an incentive approach to consumers for demand shaving in peak times. Also, the hydrogen storage system is installed in the stand-alone electrical grid to improve the main objectives. The particle swarm optimization (PSO) algorithm for energy optimization and solving objective functions is applied. In the end, numerical simulation is carried out in some case studies to confirm and supremacy of energy optimization with the participation of the hydrogen storage system and consumers. The case studies based on non-participation and participation of the storage system and consumers in energy management are implemented. The implementation of case studies examines the impact of both non-participation and participation of the storage system and consumers in energy management. The findings reveal that when the storage system and consumers actively participate, there is a significant improvement in efficiency and a substantial reduction in energy costs. Specifically, the efficiency is enhanced by 3% and the energy costs are reduced by 29.5% compared to the scenario where they do not participate.