bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral primary angiosarcoma of the lower lip mucosa: Report of a case in a 15‐year‐old boy
Authors: Arribas‐Garcia Ignacio , Domínguez Manuel Fernández , Alcalá‐Galiano Andrea , García Antonio Fernández , Valls Julio Castedo , De Rasche Enrique Niembro 
Published: February 2008

AbstractBackground.Angiosarcomas are rare soft tissue malignant tumors with dismal prognosis. Head and neck involvement is uncommon (5%) and usually affects the scalp or facial skin.Methods and Results.We present the case of an inferior lip mucosal low‐grade angiosarcoma in a 15‐year‐old boy treated exclusively with surgery. One and a half years after treatment, the patient was free of signs of recurrence. Prompt and accurate diagnosis with adequate imaging modalities and multidisciplinary treatment are crucial for optimal management of these neoplasms.Conclusion.Lip mucosal involvement is exceptional with only a few cases described in the literature, all in patients older than 60 years To our knowledge, this is the youngest patient ever reported. © 2008 Wiley Periodicals, Inc. Head Neck, 2008
Vidian nerve schwannoma with middle cranial fossa extension resected via a maxillary swing approach
Authors: Honda Keigo , Asato Ryo , Tanaka Shinzo , Endo Tsuyoshi , Nishimura Kazunari , Ito Juichi 
Published: February 2008

AbstractBackground.Vidian nerve schwannoma is an extremely rare type of facial nerve schwannoma. To the best of our knowledge, only 1 case has been reported.Methods.We report an additional case of vidian nerve schwannoma with middle cranial fossa extension in a 49‐year‐old Japanese woman. The surgical approaches for infratemporal fossa schwannomas are reviewed, and the maxillary swing approach we used is described.Results.We adopted a maxillary swing approach combined with endonasal endoscopic techniques for the resection of the lesion. Gross total resection was achieved without sacrificing the trigeminal nerves or the facial motor nerves. The postoperative course was uneventful.Conclusion.Vidian nerve schwannoma is located in the retromaxillary space. It can grow silently until it involves the median skull base extensively. The maxillary swing approach was useful in this case. © 2008 Wiley Periodicals, Inc. Head Neck, 2008
Comparison of 2D fiber network orientation measurement methods
Authors: Sander E. A. , Barocas V. H. 
Published: February 2008

AbstractThe mechanical properties of tissues, tissue analogs, and biomaterials are dependent on their underlying microstructure. As such, many mechanical models incorporate some aspect of microstructure, but a robust protocol for characterizing fiber architecture remains a challenge. A number of image‐based methods, including mean intercept length (MIL), line fraction deviation (LFD), and Fourier transform methods (FTM), have been applied to microstructural images to describe material heterogeneity and orientation, but a performance comparison, particularly for fiber networks, has not been conducted. In this study, we constructed 40 two‐dimensional test images composed of simulated fiber networks varying in fiber number, orientation, and anisotropy index. We assessed the accuracy of each method in measuring principal direction (θ) and anisotropy index (α). FTM proved to be the superior method because it was more reliable in measurement accuracy (Δθ = 2.95° ± 6.72°, Δα = 0.03 ± 0.02), faster in execution time, and flexible in its application. MIL (Δθ = 6.23° ± 10.68°, Δα = 0.08 ± 0.06) was not significantly less accurate than FTM but was much slower. LFD (Δθ = 9.97° ± 11.82°, Δα = 0.24 ± 0.13) consistently underperformed. FTM results agreed qualitatively with fibrin gel SEM micrographs, suggesting that FTM can be used to obtain image‐based statistical measurements of microstructure. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
Molecularly designed surfaces for blood deheparinization using an immobilized heparin‐binding peptide
Authors: Martins M. Cristina L. , Curtin Scott A. , Freitas Sidónio C. , Salgueiro Pedro , Ratner Buddy D. , Barbosa Mário A. 
Published: February 2008

AbstractSystemic heparinization, used during haemodialysis to prevent blood clotting on the extracorporeal circuit, leads to a high incidence of hemorrhagic complications. The adverse reactions associated with heparin neutralization using protamine sulphate justify the development of an alternative system for blood deheparinization. The main objective of this work is to design nanostructured surfaces with the capacity to bind heparin from blood in a selective way. A heparin‐binding polypeptide, composed of L‐lysine and L‐leucine (pKL), was synthesized and immobilized, in different concentrations, onto self‐assembled monolayers (SAMs) terminated with tetra(ethylene‐glycol) (EG4 SAMs). Immobilization was performed using a fixed concentration of pKL after surface activation to different degrees using a range of CDI (N,N′‐carbonyldiimidazole) concentrations. Results demonstrated that the presence of pKL increases heparin adsorption to EG4‐SAMs, independently of the pKL concentration and the way of immobilization (adsorption or covalent bound). Selectivity towards heparin was successfully achieved on SAMs with low concentrations of immobilized pKL (9–17% of pKL). Surfaces were characterized using ellipsometry, contact angle measurements, Fourier transform infrared reflection absorption spectroscopy (IRAS), atomic force microscopy, and X‐ray photoelectron spectroscopy. Heparin adsorption was assessed using IRAS and N‐sulphonate‐35S‐heparin. Therefore, this study could give a good contribution for the design of blood deheparinization devices. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Functional and phenotypic characterization of human keratinocytes expanded in microcarrier culture
Authors: Borg Danielle J. , Dawson Rebecca A. , Leavesley David I. , Hutmacher Dietmar W. , Upton Zee , Malda Jos 
Published: February 2008

AbstractSkin cells for transplantation are routinely prepared by growing patient keratinocytes in a semi‐defined cocktail of growth factors, including serum and feeder cells. However, these reagents require substantial risk remediation and can contribute to transplant rejection. Microcarrier culture is an emerging technology that may allow the elimination of feeder cells whilst facilitating expansion of cultured keratinocytes. However, the behavior of keratinocytes in microcarrier culture and the potential of these cells to form an epidermis have been poorly defined. We characterized freshly isolated human keratinocytes cultured on CultiSpher‐G® microcarriers in the absence of murine feeder cells and assessed the potential of the keratinocytes to form an epidermis in an in vitro model. In a single passage, keratinocytes multiplied 44.9‐fold in microcarrier‐bioreactor culture in 17 days, whereas two‐dimensional cultures reached confluence in 9 days and only expanded 7.4‐fold. Histological characterization of keratinocytes on the microcarriers revealed that the cells were randomly distributed within these porous structures, however, not all pores contained cells. High‐resolution microcomputed tomography imaging of the microcarriers confirmed limited interconnectivity of the pores. Immunoreactivity of specific epidermal markers was confirmed during cell expansion via immunohistochemistry. Despite the expression of differentiation markers, microcarrier‐expanded keratinocytes retained the capacity to form an epidermis, as was evaluated using an in vitro human skin equivalent model. The epidermis formed by microcarrier‐expanded keratinocytes in this model exhibited morphology similar to native skin. Significantly, the microcarrier technique successfully eliminates the need for a feeder cell layer and hence facilitates development of an improved culture system. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Analysis of the osteoinductive capacity and angiogenicity of an in vitro generated extracellular matrix
Authors: Pham Quynh P. , Kasper F. Kurtis , Mistry Amit S. , Sharma Upma , Yasko Alan W. , Jansen John A. , Mikos Antonios G. 
Published: February 2008

AbstractIn this study, the osteoinductive potential of an in vitro generated extracellular matrix (ECM) deposited by marrow stromal cells seeded onto titanium fiber mesh scaffolds and cultured in a flow perfusion bioreactor was investigated. Culture periods of 8, 12, and 16 days were selected to allow for different amounts of ECM deposition by the cells as well as ECM with varying degrees of maturity (Ti/ECM/d8, Ti/ECM/d12, and Ti/ECM/d16, respectively). These ECM‐containing constructs were implanted intramuscularly in a rat animal model. After 56 days, histologic analysis of retrieved constructs revealed no bone formation in any of the implants. Surrounding many of the implants was a fibrous capsule, which was often interspersed with fat cells. Within the pore spaces, the predominant tissue response was the presence of blood vessels and young fibroblasts or fat cells. The number of blood vessels on a per area basis calculated from a histomorphometric analysis increased as a function of the amount of ECM within the implanted constructs, with a significant difference between Ti/ECM/d16 and plain Ti constructs. These results indicate that although an in vitro generated ECM alone may not induce bone formation at an ectopic site, its use may enhance the vascularization of implanted constructs. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
Genotoxicity and cytotoxicity in multiple organs induced by titanium miniplates in Wistar rats
Authors: Piozzi Rodrigo , Ribeiro Daniel Araki , Padovan Luís Eduardo Marques , Filho Hugo Nary , Matsumoto Mariza Akemi 
Published: February 2008

AbstractInternal fixture materials are currently used as metallic biomaterials in rehabilitation of human body. Nevertheless, metal release due to corrosion phenomena appears to play a crucial role for human health. Thus, the goal of the present study was to evaluate whether liver, kidney, and lung are particularly sensitive organs for DNA damaging and cytotoxicity following implantation of internal fixture materials composed by titanium alloy in vivo. A total of 18 rats underwent surgical titanium miniplates in their tibias, being randomly distributed into three groups: 30 days, 90 days, and 180 days after implantation. A total of six animals served as negative control (animals that not received any implant). After experimental design, the lung, liver, and kidney were removed for histopatological and genotoxic analysis as depicted by H.E. stain and single cell gel (comet) assay, respectively. No significant statistically differences (p > 0.05) for DNA damaging were found to all experimental groups when compared to negative control for all organs evaluated. In addition, no remarkable morphological alterations were detected under histopathological analysis. Taken together, such results suggest that titanium miniplates are neither able to induce DNA damage in multiple organs nor to cause some abnormalities in lung, liver, and kidney. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
Antibacterial properties of Ag (or Pt)‐containing calcium phosphate coatings formed by micro‐arc oxidation
Authors: Song Won‐Hoon , Ryu Hyun Sam , Hong Seong‐Hyeon 
Published: February 2008

AbstractSilver (or platinum)‐containing calcium phosphate (hydroxyapatite (HA) and tricalcium phosphate (α‐TCP)) coatings on titanium substrates were formed by micro‐arc oxidation (MAO) and their in vitro antibacterial activity and in vitro cytotoxicity were evaluated. MAO was performed in an electrolytic solution containing β‐glycerophosphate disodium salt pentahydrate (β‐GP) and calcium acetate monohydrate (CA), and Ag and Pt were introduced in the form of AgNO3 (or CH3COOAg) and H2PtCl6, respectively. The MG63 and human osteosarcoma (HOS) cell lines were used to investigate the proliferation and differentiation behavior of the cells, respectively, whereas two strains of bacteria, Staphylococcus aureus and Escherichia coli, were used to evaluate the antibacterial activity of the coatings. The phase, morphology, and Ag content of the coatings were strongly dependent on the applied voltage and Ag precursor concentration. HA and α‐TCP phases were detected in the coatings oxidized above 400 V and the presence of Ag was confirmed by EDS. While the coatings with a high content of Ag were cytotoxic and those obtained in the Pt‐containing electrolyte had no apparent antibacterial activity, the calcium phosphate coatings obtained in the low Ag concentration electrolyte exhibited in vitro antibacterial activity but no cytotoxicity. Thus, biocompatible calcium phosphate coatings on Ti implants with antibacterial activity can be achieved by one‐step MAO. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Method for predicting sorption of small drug molecules onto polylactide
Authors: Karst David , Yang Yiqi 
Published: February 2008

AbstractA method has been developed to predict the percent sorption of small drug molecules onto polylactide (PLA). In this method, molecular modeling is first used to calculate the interaction energy between the adsorbate and PLA. Alternatively, the adsorbate‐PLA interaction energy can be calculated by summing the adsorbate functional group contributions to the interaction energy. These functional group contributions have been found in this study. The adsorbate‐PLA interaction energy is then substituted into a linear equation to predict the natural log of the sorption equilibrium constant, and the percent sorption is obtained from this value. This linear equation has been developed based on experimental sorption data for dyes on PLA. The predicted percent sorption of two small drugs on PLA has been verified with experimental data. Our method possibly could be used to select appropriate drugs for use in controlled release studies involving PLA. This method also could be used to calculate the functional group contributions to the interaction energies between other drugs and polymers and to predict their percent sorption onto those polymers, which could be used to select drugs suitable for controlled release studies involving those polymers. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Composition of corrosion layers on a magnesium rare‐earth alloy in simulated body fluids
Authors: Rettig Ralf , Virtanen Sannakaisa 
Published: February 2008

AbstractIn this study, the composition of corrosion product layers on a magnesium rare‐earth alloy in simulated body fluid (m‐SBF) containing albumin in physiological concentration is examined. The time dependence of the composition of the layer is studied. The ions from the body fluid that participate in the corrosion layer formation were identified by analyzing layers formed in different solutions that contain only some of the ions of SBF. The layer composition was analyzed by different complementary methods. We used energy dispersive X‐ray analysis, grazing incidence X‐ray diffraction, and Fourier transform infrared spectroscopy. The morphology of the corrosion layers was studied using scanning electron microscopy and light microscopy. In m‐SBF with and without albumin we found an amorphous layer of carbonated calcium phosphate with some calcium replaced by magnesium. It can be clearly shown that calcium is only deposited in the corrosion layer if phosphates are in the solution. The cross‐sections reveal that there are some sharp crevices in the substrate. The work systematically explores the nature of surface layers on magnesium rare‐earth alloys formed in complex SBFs, with the aim to elucidate the influence of specific electrolyte components on the morphology, structure, and composition of corrosion layers on Mg alloys. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
Platelet and endothelial adhesion on fluorosurfactant polymers designed for vascular graft modification
Authors: Tang Chad , Kligman Faina , Larsen Coby C. , Kottke‐Marchant Kandice , Marchant Roger E. 
Published: February 2008

AbstractA prominent failure mechanism of small diameter expanded polytetrafluoroethylene (ePTFE) vascular grafts is platelet‐mediated thrombosis. We have designed a surface modification for ePTFE consisting of a self‐assembling fluorosurfactant polymer (FSP) bearing biologically active ligands, including adhesive peptides and polysaccharide moieties. The goal of this biomimetic construct is to improve graft hemocompatibility by promoting rapid surface endothelialization, whereas minimizing platelet adhesion. Here we present a direct comparison of platelet and endothelial cell (EC) adhesion to FSPs containing one of three cell‐adhesion peptides: cyclic Arg‐Gly‐Asp‐D‐Phe‐Glu (cRGD), cyclic *Cys‐Arg‐Arg‐Glu‐Thr‐Ala‐Trp‐Ala‐Cys* (cRRE, *denotes disulfide bond cyclization), linear Gly‐Arg‐Gly‐Asp‐Ser‐Pro‐Ala (RGD), or a polysaccharide moiety: oligomaltose (M‐7), later designed to prevent nonspecific protein adhesion. Measurements of soluble peptide‐integrin binding indicated that cRRE exhibits very low affinity for the αIIbβ3 platelet fibrinogen receptor. Static and dynamic adhesion of washed, activated platelets on FSP‐modified surfaces revealed that M‐7 and cRRE promote significantly less platelet adhesion compared to RGD and cRGD FSPs, whereas EC adhesion was similar on all peptide FSPs and minimal on M‐7 FSP. These results illustrate the potential for ligands presented in a FSP surface modification to selectively adhere ECs with limited platelet attachment. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res 2009
Fibroblast growth factor‐2‐apatite composite layers on titanium screw to reduce pin tract infection rate
Authors: Mutsuzaki Hirotaka , Ito Atsuo , Sakane Masataka , Sogo Yu , Oyane Ayako , Ochiai Naoyuki 
Published: February 2008

AbstractFibroblast growth factor‐2 (FGF‐2)‐apatite composite layers were formed on anodically oxidized titanium screws to improve bone‐screw interface strength and to reduce pin tract infection rate through enhanced skin tissue healing in external fixation. A calcium‐containing solution supplemented with FGF‐2, a phosphate‐containing solution, and a sodium bicarbonate solution were mixed at a Ca/P molar ratio of 2.0 to prepare a calcium phosphate solution supersaturated with respect to calcium phosphates. Screws were individually immersed in 10 mL of the calcium phosphate solution at 37°C for 2 days. Low‐crystalline apatite layers incorporating FGF‐2 were formed on the screw surface at FGF‐2 concentrations in the supersaturated calcium phosphate solution equal to or lower than 10 μg/mL. The amounts of FGF‐2 immobilized on the screws ranged from 2.3‐ to 2.4‐μg per screw. The immobilized FGF‐2 retained biological activity, as demonstrated by NIH3T3 cell proliferation. Titanium screws with the composite layer were percutaneously implanted into the bilateral proximal tibial metaphyses in rabbits for 4 weeks. The titanium screws with the composite layer formed at the optimum FGF‐2 concentration showed a significantly higher bone‐screw interface strength and a lower pin tract infection rate than those without the composite layer: the extraction torque and infection rates were respectively 0.230 ± 0.073 Nm and 43.8% for the screws with the composite layer, and 0.170 ± 0.056 Nm and 93.8% for those without the composite layer. Therefore, titanium screws with the FGF‐2‐apatite composite layer are useful for improving bone‐screw interface strength and infection resistance in external skeletal fixation. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008
CYR61 is a novel gene associated with temperature‐dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line
Authors: Hirayama Makoto , Ahsan Md. Nazmul , Mitani Hiroshi , Watabe Shugo 
Published: February 2008

AbstractA microarray comprising 3,514 cDNAs was constructed from a medaka EST library to elucidate the transcriptional responses associated with temperature shift from 25 to 15°C in a medaka cell line. Microarray analysis revealed that the mRNA levels of 313 clones were significantly different in at least one combination of different incubation periods up to 7 days at a given incubation temperature or between 25 and 15°C at a given incubation period (P < 0.05). These genes are known to be associated with various biological processes including morphogenesis, cell proliferation and response to stress. A number of genes encoding proteins which localize in extracellular areas were apparently up‐regulated at 15°C, whereas those localizing in intracellular areas were down‐regulated at this temperature. In addition, while a number of genes represented long‐term expression changes, only a few responded to short‐term inductions. A typical example was CYR61, a multifunctional matricellular signaling modulator, the mRNA levels of which increased after temperature shift from 25 to 15°C in 3 h, and then decreased rapidly to near the original level within 12 h. Another series of analyses by quantitative reverse transcription‐PCR revealed that the mRNA levels of CYR61 at 5°C were significantly higher even at 24 h after temperature shift compared to those of the cells successively maintained at 25°C. These analyses suggest that remodeling and reorganizing of extracellular structure of cells are important to offset the low temperature effect and CYR61 is considered to be a novel gene associated with temperature response in poikilotherms. J. Cell. Biochem. 104: 1297–1310, 2008. © 2008 Wiley‐Liss, Inc.
Activation of the ERK pathway in osteoblastic cells, role of gremlin and BMP‐2
Authors: Zanotti Stefano , Smerdel‐Ramoya Anna , Stadmeyer Lisa , Canalis Ernesto 
Published: February 2008

AbstractGremlin is a glycoprotein that binds and antagonizes the actions of bone morphogenetic proteins (BMPs) ‐2, ‐4, and ‐7. Gremlin appears to activate the extracellular regulated kinase (ERK) pathway in endothelial and tumor cells, and as a consequence to have direct cellular effects. To determine whether gremlin has direct effects in osteoblasts, independent of its BMP binding activity, we examined its effects in ST‐2 murine stromal cell lines and in primary cultures of murine calvarial osteoblasts. Gremlin did not activate Signaling mothers against decapentaplegic (Smad), and suppressed the BMP‐2 induced Smad 1/5/8 phosphorylation and the transactivation of the BMP/Smad reporter construct 12xSBE‐Oc‐pGL3, confirming its BMPs antagonizing activity. Neither gremlin nor BMP‐2 induced ERK 1/2 activation in ST‐2 cells or calvarial osteoblasts. Moreover, slight changes in culture conditions induced the phosphorylation of ERK independent from BMP or gremlin exposure. In conclusion, gremlin inhibits BMP‐2 signaling and activity, and does not have independent actions on ERK signaling in osteoblasts. Consequently, gremlin activity in osteoblasts can be attributed only to its BMP antagonizing effects. J. Cell. Biochem. 104: 1421–1426, 2008. © 2008 Wiley‐Liss, Inc.