bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Posterior Tibial Slope on Outcomes After Posterior Cruciate Ligament Reconstruction
Authors: Conyer Ryan T. , Allen Tyler G. , Reinholz Anna K. , Krych Aaron J. , Okoroha Kelechi R. , Stuart Michael J. , Levy Bruce A. 
Published: March 2024

Background: Increased posterior tibial slope (PTS) leads to a relative anterior translation of the tibia on the femur. This is thought to decrease the stress on posterior cruciate ligament (PCL) reconstruction (PCLR) grafts. Purpose/Hypothesis: The purpose of this study was to analyze the effect of PTS on knee laxity, graft failure, and patient-reported outcome (PRO) scores after PCLR without concomitant anterior cruciate ligament reconstruction (ACLR). It was hypothesized that patients with higher PTS would have less knee laxity, fewer graft failures, and better PROs compared with patients with lower PTS. Study Design: Case-control study; Level of evidence, 3. Methods: All patients who underwent PCLR between 2001 and 2020 at a single institution were identified. Patients were excluded if they underwent concomitant or prior ACLR or proximal tibial osteotomy, were younger than 18 years, had <2 years of in-person clinical follow-up, and did not have documented PRO scores (Lysholm score and International Knee Documentation Committee [IKDC] score). Data were collected retrospectively from a prospectively gathered database. PTS measurements were recorded from perioperative lateral knee radiographs. A linear regression model was created to analyze PTS in relation to PRO scores. Patients with a grade 1 (1-5 mm) or higher posterior drawer were compared with those who had a negative posterior drawer. Results: A total of 37 knees met inclusion criterion; the mean age was 30.7 years at the time of surgery. The mean clinical follow-up was 5.8 years. No significant correlation was found between either the Lysholm score or the IKDC score and the PTS. Twelve knees (32.4%) had a positive posterior drawer at final follow-up. The mean PTS in knees with a positive posterior drawer was 6.2°, whereas that for knees with a negative posterior drawer was 8.3° ( P = .08). No significant differences in PRO scores were identified for knees with versus knees without a positive posterior drawer. No documented graft failures or revisions were found. Conclusion: No significant differences were found in PROs or graft failure rates based on PTS at a mean of 5.8 years after PCLR. Increased tibial slope trended toward being protective against a positive posterior drawer, although this did not reach statistical significance.
Association of 5-Year Hip Arthroscopy Outcomes with Hip Morphology and Cartilage Status at Time of Surgery: A National Registry Study With HAGOS Outcomes in 281 Patients With Femoroacetabular Impingement Syndrome
Authors: Richter Camilla , Ishøi Lasse , Kraemer Otto , Hölmich Per , Thorborg Kristian 
Published: March 2024

Background: The effects of specific bony hip morphologies, cam and dysplasia, and cartilage damage on mid- and long-term (≥5 years) patient-reported outcomes (PROs) are understudied. Purpose: To investigate if changes in PROs from preoperatively to 5 years after hip arthroscopy are associated with preoperative bony hip morphology and cartilage status in patients with femoroacetabular impingement syndrome. Study Design: Cohort study; Level of evidence, 3. Methods: Patients were identified in the Danish Hip Arthroscopy Registry. Hip and groin function was assessed from preoperatively to 5 years postoperatively with the Copenhagen Hip and Groin Outcome Score (HAGOS) Activities of Daily Living (ADL) and Sports and Recreation (Sport) subscales. Morphology was defined using the anterior alpha angle (AA) and lateral center-edge angle (LCEA) as follows: mild to moderate cam (55°≤ AA < 78°), severe cam (AA ≥ 78°), pincer (LCEA > 39°), and borderline dysplasia (20°≤ LCEA < 25°). Joint space width (JSW) was defined as slightly reduced (3.1 mm ≤ JSW ≤ 4 mm) or severely reduced (2.1 mm ≤ JSW ≤ 3 mm). Acetabular cartilage status was defined by modified Beck grades 0 to 4 and femoral head cartilage status by International Cartilage Regeneration & Joint Preservation Society grades 0 to 4. Acetabular and femoral cartilage injury areas were categorized as <1, 1 to 2, or >2 cm2. Multiple regression analyses assessed adjusted associations between hip morphology and cartilage injuries with improvement in HAGOS-ADL and HAGOS-Sport. Results: The study included 281 patients (age, 35 ± 10 years; 52.3% female). No cam and mild-to-moderate cam were associated with greater improvement in HAGOS-ADL (16 points [ P = .002] and 7 points [ P = .038], respectively) compared with severe cam. Normal JSW was associated with greater improvement in HAGOS-ADL (21 points; P = .026) compared with severely reduced JSW. Femoral head cartilage injury area <1 cm2 was associated with greater improvements in HAGOS-ADL (17 points; P = .03) and HAGOS-Sport (21 points; P = .035) compared with femoral cartilage injury area >2 cm2. Conclusion: Patients having no-to-moderate cam morphology, normal JSW, or femoral head cartilage injury area <1 cm2 had greater improvement in PROs 5 years after hip arthroscopy compared with patients having severe cam morphology, severely reduced JSW, or femoral cartilage injury area >2 cm2.
A Comparison Between In-Person and Virtual Communication Skills OSCE for Medical Students
Authors: Choi Alex , Murtha Tanya D. , Morrison Laura J. , Talwalkar Jaideep S. 
Published: March 2024

Objectives This study investigates the effectiveness of a virtual format of an advanced communication skills observed structured clinical examination (OSCE) for senior medical students in comparison to an in-person format. The study also examines the emotional support students experience in the virtual setting. Our analysis was based on quantitative data collected through objective checklists and post-OSCE survey results. Methods The virtual OSCE was a revision of an earlier in-person formative advanced communication skills OSCE for fourth-year medical students. Student performances were assessed by self and peers using objective checklists—the modified Master Interview Rating Scale (mMIRS) and Communication Behavior Checklist (CBC). The mMIRS measured interview process such as avoiding jargon and demonstrating empathy. The CBC examined interview content which included tasks specific to the content of the case. The OSCE was followed by a faculty-led debrief and quantitative survey. The virtual OSCE was conducted in 2021, and the results of the checklists and survey were compared with those collected from two earlier in-person OSCEs. Results Eighty-three students participated in the virtual OSCE. There was no difference in mMIRS scores between the virtual and in-person OSCE. Overall CBC scores were lower in the virtual OSCE compared to in-person ( p < 0.05). Sixty-seven out of 83 (80.7%) students completed the post-OSCE survey. There were no differences between the virtual and in-person OSCE in terms of educational value, whether the OSCE would change the way participants talk to patients, and preparedness to have serious conversations with patients. All respondents somewhat or strongly agreed with feeling emotionally supported during the virtual OSCE. Conclusion The virtual format was a suitable alternative to an in-person, formative, advanced communication skills OSCE for medical students. The virtual OSCE was educationally effective and was met with student satisfaction and a sense of emotional support. Future virtual iterations must ensure adequate instruction on interview content.
Prevalence, causes, and clinical associations of anemia in patients with systemic sclerosis: A cohort study
Authors: Gachet Benoit , Jouvray Mathieu , Koether Vincent , Collet Aurore , Morell-Dubois Sandrine , Sanges Sébastien , Sobanski Vincent , Hachulla Eric , Launay David 
Published: March 2024

Background: Anemia is considered a risk factor of severity in systemic sclerosis. Yet, limited data are available on the frequency and causes of anemia in systemic sclerosis. The objectives of our study were to determine the frequency and causes of anemia in systemic sclerosis, to analyze the clinical and biological characteristics of patients with anemia, and to assess the association between anemia and systemic sclerosis prognosis. Methods: We conducted a prospective single-center study from January 2017 to May 2022. Patients underwent a hemoglobin assay and a standardized etiological workup to determine the causes of anemia at the initial visit. Clinical and biological parameters were compared between patients with anemia and those with normal hemoglobin levels. We followed up patients until May 2023 and compared their survival. Results: A total of 502 systemic sclerosis patients, including 107 diffuse cutaneous systemic sclerosis, were included. At enrollment, 100 patients had anemia. The primary cause of anemia was iron deficiency (40%). Among the 32 patients with iron deficiency-associated anemia who underwent gastrointestinal exploration, 56% had digestive bleeding, with upper gastrointestinal tract involvement being the main cause (90%). Patients with anemia at enrollment had higher systemic sclerosis severity scores and more gastrointestinal symptoms compared to patients without anemia (p < 0.05). They exhibited higher systolic pulmonary artery pressure, lower anemia-corrected diffusing capacity for carbon monoxide, and lower forced vital capacity (p < 0.05). During follow-up, 65 patients (14.8%) died. After adjusting for age, systemic sclerosis subtypes, forced vital capacity, and pulmonary arterial hypertension, anemia at inclusion was associated with a higher mortality rate (hazard ratio: 1.94 (95% confidence interval: 1.39–2.48), p = 0.02). Conclusion: Our study shows a high frequency of anemia among patients with systemic sclerosis. Most anemias are due to iron deficiency, and gastrointestinal exploration can identify bleeding in the majority of the cases. In addition, our study confirms that systemic sclerosis patients with anemia have a more severe disease and a higher mortality rate.
Criminal Justice Responses to Sex Trafficking in West Bengal, India: A Representative Study from Victims’ Lenses
Authors: Chakraborty Ankita , Dube Dipa 
Published: March 2024

Known to be the oldest crime on earth, human trafficking has existed as an illegal industry from time immemorial. Statistics reveal that every year, 1–2 million men, women and children are trafficked worldwide, and about 0.22 million (225,000) are from South Asia. Amongst the South-Asian countries, India faces a high-ceilinged rate of sex trafficking and although it made its anti-trafficking interventions quite early, with the inclusion of anti-trafficking provisions in its domestic legislation and signed the United Nations Protocol, yet, the problem persisted and assumed alarming proportions. The principal argument that has been offered for the inept human trafficking responses in India is that the different facets of prevention, protection and prosecution centred on the human rights priorities of the victims are not adequately met. The present article is based on an empirical study conducted by the authors to understand the underpinnings of the working of the Indian criminal justice system (CJS) from the lens of victim’s experiences in West Bengal, India. West Bengal is one of the states located in the eastern part of the country, which shares its borders with Bangladesh and Nepal, and acts as an epicentre of sex trafficking in India. To determine whether the anti-trafficking interventions in India, which are largely based on the rhetoric of raid-to-rescue, are attuned to the specific needs of the sex trafficked survivors; the authors attempted to assess their encounters with and confidence in the CJS. They used cross-sectional surveys to collect qualitative data from 40 victims ( n = 40) across three after-care facilities in West Bengal, namely, Sanlaap, International Justice Mission and Mahima. The purpose of using this phenomenological study was to describe the phenomenon as participant-respondents experience them. In conclusion, the authors pinpointed the necessary interventions required at the legal and procedural levels to make the ICJS more responsive towards the needs of the sex-trafficked victims.
Enhanced MAPK signaling induced by CSF3R mutants confers dependence to DUSP1 for leukemic transformation
Authors: Kesarwani Meenu , Kincaid Zachary , Azhar Mohammad , Azam Mohammad 
Published: March 2024

Abstract
Elevated MAPK and the JAK-STAT signaling play pivotal roles in the pathogenesis of chronic neutrophilic leukemia and atypical chronic myeloid leukemia. Although inhibitors targeting these pathways effectively suppress the diseases, they fall short in providing enduring remission, largely attributed to the cytostatic nature of these drugs. Even combinations of these drugs are ineffective in achieving sustained remission. Enhanced MAPK signaling besides promoting proliferation and survival triggers a proapoptotic response. Consequently, malignancies reliant on elevated MAPK signaling use MAPK feedback regulators to intricately modulate the signaling output, prioritizing proliferation and survival while dampening the apoptotic stimuli. Herein, we demonstrate that enhanced MAPK signaling in granulocyte colony-stimulating factor 3 receptor (CSF3R)–driven leukemia upregulates the expression of dual specificity phosphatase 1 (DUSP1) to suppress the apoptotic stimuli crucial for leukemogenesis. Consequently, genetic deletion of Dusp1 in mice conferred synthetic lethality to CSF3R-induced leukemia. Mechanistically, DUSP1 depletion in leukemic context causes activation of JNK1/2 that results in induced expression of BIM and P53 while suppressing the expression of BCL2 that selectively triggers apoptotic response in leukemic cells. Pharmacological inhibition of DUSP1 by BCI (a DUSP1 inhibitor) alone lacked antileukemic activity due to ERK1/2 rebound caused by off-target inhibition of DUSP6. Consequently, a combination of BCI with a MEK inhibitor successfully cured CSF3R-induced leukemia in a preclinical mouse model. Our findings underscore the pivotal role of DUSP1 in leukemic transformation driven by enhanced MAPK signaling and advocate for the development of a selective DUSP1 inhibitor for curative treatment outcomes.
Neutrophil functions in patients with neutropenia due to glycogen storage disease type 1b treated with empagliflozin
Authors: Kaczor Magdalena , Malicki Stanislaw , Folkert Justyna , Dobosz Ewelina , Bryzek Danuta , Chruscicka-Smaga Barbara , Greczan Milena , Wesół- Kucharska Dorota , Piątosa Barbara , Samborowska Emilia , Madzio Joanna , Książyk Janusz , Ehmke vel Emczyńska Ewa , Hajdacka Małgorzata , Potempa Jan , Młynarski Wojciech , Rokicki Dariusz , Veillard Florian 
Published: March 2024

Abstract
Neutropenia and neutrophil dysfunction in glycogen storage disease type 1b (GSD1b) are caused by the accumulation of 1,5-anhydroglucitol-6-phosphate in granulocytes. The antidiabetic drug empagliflozin reduces the concentration of 1,5-anhydroglucitol (1,5-AG), thus restoring neutrophil counts and functions, leading to promising results in previous case reports. Here, we present a comprehensive analysis of neutrophil function in 7 patients with GSD1b and 11 healthy donors, aiming to evaluate the immediate (after 3 months) and long-term (after 12 months) efficacy of empagliflozin compared with the reference treatment with granulocyte-colony stimulating factor (G-CSF). We found that most patients receiving G-CSF remained neutropenic with dysfunctional granulocytes, whereas treatment with empagliflozin increased neutrophil counts and improved functionality by inhibiting apoptosis, restoring phagocytosis and the chemotactic response, normalizing the oxidative burst, and stabilizing cellular and plasma levels of defensins and lactotransferrin. These improvements correlated with the decrease in serum 1,5-AG levels. However, neither G-CSF nor empagliflozin overcame deficiencies in the production of cathelicidin/LL-37 and neutrophil extracellular traps. Given the general improvement promoted by empagliflozin treatment, patients were less susceptible to severe infections. G-CSF injections were therefore discontinued in 6 patients (and the dose was reduced in the seventh) without adverse effects. Our systematic analysis, the most extensive reported thus far, has demonstrated the superior efficacy of empagliflozin compared with G-CSF, restoring the neutrophil population and normal immune functions. This trial was registered as EudraCT 2021-000580-78.
The North Penninic Bündnerschiefer and Flysch of the Prättigau (Swiss Alps) revisited
Authors: Winkler Wilfried 
Published: March 2024

AbstractDuring the re-mapping of the area for the Geological Atlas of Switzerland, a significant stratigraphic unconformity was discovered in the North Penninic (Valais) Bündnerschiefer and the Flysch series of the northern Prättigau. It separates different units of the Cretaceous Bündnerschiefer from the Palaeogene Flysch. We explain this observation by a basin conversion from extension to compression, which caused the initial deformation of the Bündnerschiefer in an accretionary wedge. Interlinked return-flow has created a new heterogeneous substrate for the flysch sediments and explains the different types of unconformities. The basin conversion coincided with high-grade metamorphism in the vicinity of the the South Penninic suture and the Austroalpine units, and the increased exhumation in the Austroalpine nappe stack. Detrital zircon dating confirms also a change from European to Austroalpine detrital sources in the flysch sandstones. We discuss a palaeotectonic model leading to hP/lT metamorphism of the Bündnerschiefer in the Late Eocene (c. 42 Ma). It appears that the flysch formations were also involved, but to a lesser degree by tectonic deformation from the late Early Eocene onwards, as the pervasive folding characteristic of the Bündnerschiefer is absent. This has been followed by a phase of S-directed backfolding. During the Oligocene and Miocene, more extensive deformation occurred by SE to NW compression and finally by probable westward thrusting and folding. Our main theme is the transition from passive to active continental margins, which in Alpine plate tectonic framework corresponds to the transition to flysch sedimentation by basin conversion. Our results show that the simultaneity of the transition from extension to compression, as indicated by the accumulation of flysch, shifted in time from south to north in the Alpine Tethys.
Emerging roles of prominin-1 (CD133) in the dynamics of plasma membrane architecture and cell signaling pathways in health and disease
Authors: Pleskač Petr , Fargeas Christine A. , Veselska Renata , Corbeil Denis , Skoda Jan 
Published: March 2024

AbstractProminin-1 (CD133) is a cholesterol-binding membrane glycoprotein selectively associated with highly curved and prominent membrane structures. It is widely recognized as an antigenic marker of stem cells and cancer stem cells and is frequently used to isolate them from biological and clinical samples. Recent progress in understanding various aspects of CD133 biology in different cell types has revealed the involvement of CD133 in the architecture and dynamics of plasma membrane protrusions, such as microvilli and cilia, including the release of extracellular vesicles, as well as in various signaling pathways, which may be regulated in part by posttranslational modifications of CD133 and its interactions with a variety of proteins and lipids. Hence, CD133 appears to be a master regulator of cell signaling as its engagement in PI3K/Akt, Src-FAK, Wnt/β-catenin, TGF-β/Smad and MAPK/ERK pathways may explain its broad action in many cellular processes, including cell proliferation, differentiation, and migration or intercellular communication. Here, we summarize early studies on CD133, as they are essential to grasp its novel features, and describe recent evidence demonstrating that this unique molecule is involved in membrane dynamics and molecular signaling that affects various facets of tissue homeostasis and cancer development. We hope this review will provide an informative resource for future efforts to elucidate the details of CD133’s molecular function in health and disease.
Durable anti-fog micro-nano structures fabricated by laser ablation of aluminum film on resin/glass
Authors: Cui Hongtao , Teng Chao , Xie Xinyi , Qi Xiaowen 
Published: March 2024

AbstractThis study presents a technique for processing transparent glass and resin substrates using a low-cost laser marker to create a micro-nano-structured surface with exceptional anti-fog properties. The approach involved depositing an aluminum (Al) film on the transparent substrates as an absorbing layer, followed by rapid laser marker ablation. This ablation process effectively removed the majority of the Al film, resulting in the formation of hierarchical hillock-hollow micro-structures and the dispersion of Al-based nano-particles throughout the surface. The resulting structure on resin glasses demonstrated anti-fog performance even after 629 days storage in the laboratory, which marked the longest antifog record. It exhibited impressive antifog property without visible degradation for the first 9 months, which though degraded substantially afterwards. Furthermore, the micro-nano structure played a key role in reducing the contact angle of the surface. The contact angle experienced a significant reduction from a value of 64° for the control resin to 6.9° for the treated resin, while it was reduced from 44° for the control glass to 0° for the treated glass, indicating superhydrophilicity. This 0° superhydrophilic state persisted for a period of 25 days.
Deciphering the Genetic Basis of Allelopathy in japonica Rice Cultivated in Temperate Regions Using a Genome-Wide Association Study
Authors: García-Romeral Julia , Castanera Raúl , Casacuberta Josep , Domingo Concha 
Published: March 2024

AbstractAllelopathy has been considered as a natural method of weed control. Despite the nature of allelochemical compounds has been studied, little is known about the genetic basis underlying allelopathy. However, it is known that rice exhibits diverse allelopathic potentials across varieties, and breeding for rice plants exhibiting allelopathic potential conferring an advantage against weeds in paddy fields would be highly desirable. Knowledge of the gene factors and the identification of the genomic regions responsible for allelopathy would facilitate breeding programs. Taking advantage of the existing genetic diversity in rice, particularly in temperate japonica rice, we conducted a comprehensive investigation into the genetic determinants that contribute to rice allelopathy. Employing Genome-Wide Association Study, we identified four Quantitative Trait Loci, with the most promising loci situated on chromosome 2 and 5. Subsequent inspection of the genes located within these QTLs revealed genes associated with the biosynthesis of secondary metabolites such as Phenylalanine Ammonia Lyase (PAL), a key enzyme in the synthesis of phenolic compounds, and two genes coding for R2R3-type MYB transcription factors. The identification of these two QTLs associated to allelopathy in rice provides a useful tool for further exploration and targeted breeding strategies.