bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of dopamine and its relativity of baicalin in rat nuclei after intravenous administration of flavonoids from Scutellariae radix
Authors: Chen Long , Zhang Lujun , Wang Xueli , Lin Han , Du Lijun 
Published: November 2006

AbstractBaicalin is an active component of Scutellariae radix extracts. It can cross the blood–brain barrier and distribute in cerebral nuclei. However, its mechanism and the effects on the nuclei where it accumulates remain unclear. We used an HPLC–electrochemical detection method to determine the dopamine in cerebral nuclei after intravenous administration of flavonoids from Scutellariae radix and analyzed the relativity between baicalin and DA in cerebral nuclei. We found that the dopamine system is another target system of brain function which baicalin acts on. Copyright © 2006 John Wiley & Sons, Ltd.
HPLC determination and pharmacokinetic study of tenatoprazole in dog plasma after oral administration of enteric‐coated capsule
Authors: Liu Pei , Sun Bo , Lu Xiumei , Qin Feng , Li Famei 
Published: November 2006

AbstractA simple, sensitive and selective high‐performance liquid chromatographic (HPLC) method with UV detection (306 nm) was developed and validated for determination of tenatoprazole, a novel proton‐pump inhibitor, in dog plasma. Tenatoprazole and internal standard (pantoprazole) were extracted into diethyl ether and separated using an isocratic mobile phase of 10 mm phosphate buffer (pH4.7)–acetonitrile (70:30, v/v) on a Diamonsil C18 column (150 × 4.6 mm, 5 µm). The retention times for tenatoprazole and internal standard were 7.1 and 12.3 min, respectively. No endogenous interferences were observed. This HPLC method was fully validated. The lower limit of quantitation was 20 ng/mL, with a relative standard deviation of less than 20%. A linear range of 0.02–5.0 µg/mL was established. The interday and intraday precisions were within RSD 13.4–10.1 and 4.6–1.4%, respectively. This method developed can be easily applied to the pharmacokinetic study of tenatoprazole in dog plasma after oral administration of an enteric‐coated capsule. The plasma concentration of tenatoprazole from six dogs showed a mean Cmax of 2.63 µg/mL at Tmax of 1.89 h. The bioavailability of tenatoprazole was improved by administration of enteric‐coated capsule. Copyright © 2006 John Wiley & Sons, Ltd.
Prognostic factors in patients with unresectable locally advanced pancreatic adenocarcinoma treated with chemoradiation
Authors: Krishnan Sunil , Rana Vishal , Janjan Nora A. , Abbruzzese James L. , Gould Morris S. , Das Prajnan , Delclos Marc E. , Palla Shana , Guha Sushovan , Varadhachary Gauri , Evans Douglas B. , Wolff Robert A. , Crane Christopher H. 
Published: November 2006

AbstractBACKGROUND.Although patients with locally advanced pancreatic cancer (LAPC) have an extremely poor prognosis, they are a heterogeneous group. Prognostic factors are inadequately defined for disease‐free survival and overall survival in patients with LAPC who are receiving chemoradiation, so more definitive prognostic factors would be very useful for designing clinical trials.METHODS.Between December 1993 and July 2005, 247 patients with nonmetastatic LAPC were treated at M. D. Anderson Cancer Center (Houston, Tex) with concurrent chemoradiation (CRT). Median radiation dose was 30 Gy (range, 15–52.2 Gy). Radiosensitizers included 5‐fluorouracil (54%), gemcitabine (33%), and capecitabine (13%). Actuarial univariate and multivariate statistical methods were used to determine significant prognostic factors for disease‐free survival and overall survival.RESULTS.Median follow‐up was 4.3 months (range, 1–63 months). Median disease‐free survival and overall survival were 4.2 months and 8.5 months, respectively. On univariate analysis, prognostic factors for improved disease‐free survival were a Karnofsky performance scale (KPS) status of >80 (P< .01) and a hemoglobin (Hgb)level at presentation of ≥12 (P= .03). On multivariate analysis, KPS was the only independent prognostic factor for disease‐free survival. Median disease‐free survival was 4.9 months among patients with a KPS score of >80 and was 3.9 months among those with a KPS score of ≤80. On univariate analysis, prognostic factors for improved overall survival were an Hgb level of ≥12 (P= .02), KPS>80 (P< .001), and <5% weight loss (P= .03). On multivariate analysis, Hgb and KPS were independent prognostic factors for overall survival.CONCLUSIONS.In the current study, KPS score was an independent prognostic factor for disease‐free and overall survival among patients treated with chemoradiation for LAPC. The pretreatment Hgb level was an additional independent prognostic factor for overall survival. Cancer 2006. © 2006 American Cancer Society.
Broad‐Complex, E74, and E75 early genes control DNA puff BhC4‐1 expression in prepupal salivary glands
Authors: Basso L.R. , Neves M. de C. , Monesi N. , Paçó‐Larson M.L. 
Published: November 2006

AbstractThe DNA puff BhC4‐1 gene of the sciarid Bradysia hygida is induced in salivary glands prior to the pupal molt as a secondary response to the increase in ecdysone titers. Previous studies demonstrated that the BhC4‐1 promoter is activated in transgenic Drosophila melanogaster salivary glands as a late response to the ecdysone peak that triggers metamorphosis, revealing that this aspect of BhC4‐1 transcriptional regulation is conserved in the Drosophila background. To identify regulators of BhC4‐1 expression, we utilized a candidate gene approach and tested the roles of the ecdysone‐induced genes BR‐C, E74, and E75. Our results reveal that the BR‐C Z3 isoform is essential for BhC4‐1‐lacZ induction in prepupal salivary glands and constitute the first demonstration of the participation of early genes products on DNA puff genes regulation. genesis 44:505–514, 2006. © 2006 Wiley‐Liss, Inc.
Determination of pKa values of diastereomers of phosphinic pseudopeptides by CZE
Authors: Koval Dušan , Kašička Václav , Jiráček Jiří , Collinsová Michaela 
Published: November 2006

AbstractA CE method was used for the determination of acidity constants (pKa) of a series of ten phosphinic pseudopeptides, which varied in number and type of ionogenic groups. Effective electrophoretic mobilities were measured in the 1.8–12.0 pH range in the BGEs of constant ionic strength of 25 mM. Effective electrophoretic mobilities, corrected to standard temperature of 25°C, were subjected to non‐linear regression analysis and the obtained apparent pKa values were recalculated to thermodynamic pKa's by extrapolation to zero ionic strength according to the extended Debye‐Hückel model. The pKa values of the phosphinic acid group fell typically in the 1.5–2.25 interval, C‐terminal carboxylic groups in the 2.94–3.50 interval, carboxylic groups of the lateral chain of glutamate and aspartate in the 4.68–4.97 interval, imidazolyl moiety of histidine in the 6.55–8.32 interval, N‐terminal amino groups in the 7.65–8.28 interval and ε‐amino group of the lateral chain of lysine in the 10.46–10.61 interval. Further, separation of diastereomers of the phosphinic pseudopeptides was investigated in achiral BGEs. Evaluation of the resolution of the diastereomers as a function of pH of the BGE revealed that most suitable pH region for separation of the diastereomers is around the pKa values of the central phosphinic acid group of the pseudopeptides. Successful separation of some diastereomers was, however, achieved in the neutral and alkaline BGEs as well.
Miniaturized liquid core waveguide‐based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids
Authors: Kostal Vratislav , Zeisbergerova Marta , Hrotekova Zuzana , Slais Karel , Kahle Vladislav 
Published: November 2006

AbstractA miniaturized post‐column fluorimetric detection cell for capillary separation methods based on optical fibers and liquid core waveguides (LCWs) is described. The main part of the detection cell is a fused‐silica capillary coated with Teflon AF serving as an LCW. The optical fibers are used both for coupling the excitation source with the detection domain in the LCW and for the axial fluorescence collection from the LCW end. The latter fiber is connected with a compact CCD spectrometer that serves for the rejection of the scattered excitation light and for the fluorescence signal detection. The proposed design offers a compact fluorescence detector for various microcolumn separation techniques without optical elements such as filters or objectives. Moreover, its construction and optical adjustment are very simple and the whole system is highly miniaturized. The function of the detection cell is demonstrated by CE of amino acids labelled by fluorescein‐based tags. Separations of different standard amino acid mixtures and plasma samples are presented. The comparison of plasma amino acid levels of individuals being in good health with those of patients with inherited metabolic disorders is also shown.
In vivo volatiles emission and essential oils from different organs and pollen of Cistus albidus from Caprione (Eastern Liguria, Italy)
Authors: Maccioni Simonetta , Baldini Rosa , Cioni Pier Luigi , Tebano Marianna , Flamini Guido 
Published: November 2006

AbstractThe composition of the essential oils obtained from the whole flowering tops and from isolated flowers and leaves of Cistus albidus L. (Cistaceae) has been investigated. The three essential oils were constituted mainly by sesquiterpene derivatives, but they showed quite different compositions. In the flowering tops and flowers the main constituents were α‐zingiberene, α‐cadinol and ar‐curcumene, while in the leaves the principal compounds were α‐cadinol, juniper camphor, germacrene D and spathulenol. Furthermore, the volatiles emitted in vivo by whole flowers, petals, sepals, pollen and leaves were investigated by means of SPME. Also in these analyses, sesquiterpenes were the main constituents but with a clear dominance of hydrocarbon derivatives. The whole flowers, petals, sepals and leaves of C. albidus showed a similar emission pattern of volatiles, whereas the pollen showed a very different profile. Copyright © 2006 John Wiley & Sons, Ltd.
Isolierung und Charakterisierung eines Pektinase‐Inhibitors aus grünen Bohnen (Phaseolus vulgaris)
Authors: Göbel H. , Bock W. 
Published: November 2006

AbstractAus grünen Bohnen wird durch Anwendung konventioneller Methoden der Proteinreinigung, eingeschlossen Acetonbehandlung, Extraktion mit Kochsalz, Fraktionierung mit Ammoniumsulfat, Chromatographie an SEPHADEX G‐50, CM‐SEPHADEX, SEPHADEX G‐200 und Rechromatographie, ein Pektinase‐Inhibitor isoliert, dessen spezifische Aktivität 160mal höher ist als im Inhibitorrohextrakt. Der in 20%iger Ausbeute gewonnene Inhibitor 1st ein arabinosehaltiges basisches Glykoprotein mit einem Molekulargewicht um 40000 und mit einem isoelektrischen Punkt von pH 9,3. Er ist weitgehend stabil im sauren pH‐Bereich und bis zu einer Temperatur von etwa 50 °C. Eine hämagglutinierende Aktivität konnte nicht nachgewiesen werden. Der Pektinase‐Inhibitor gehört zu einer Gruppe von pflanzeneigenen Wirkstoffen mit ausgeprägten spezifischen Hemmeigenschaften gegenüber mikrobiellen pektinolytischen Enzymen vom Endo‐Typ.
Untersuchungen zur Verbesserung der Qualität von Rapssamen durch gemeinsame Silierung mit Futterzuckerrüben 1. Mitt. Qualitätsbeurteilung der Silagen
Authors: Kozlowski M. , Koterska B. , Zaworowski K. , Kozłowska H. , Borowska J. 
Published: November 2006

AbstractDie im technischen Maßstab durchgeführten Untersuchungen zeigten, daß der Zusatz von zu Flocken verarbeiteten Rapssamen zu Futterzuckerrüben den Futterwert und die Qualität der Silagen erhöht. Außerdem wurde festgestellt, daß mit steigendem Anteil an Rapssamen die Entwicklung von Hefen gehemmt und die Vermehrung von Säurebildnern verzögert war. Die mikrobiologische Analyse weist darauf hin, daß der Zusatz von Rapssamen zu Rüben 10% nicht überschreiten sollte.
Untersuchungen zur Maillard‐Reaktion zwischen β‐Lactoglobulin und Lactose. 2. Mitt. Der Einfluß der Thiol‐ und Disulfidgruppen auf die Beschaffenheit der makromolekularen Reaktionsprodukte
Authors: Ludwig E. 
Published: November 2006

AbstractIst an der Maillard‐Reaktion ein Protein als Aminokomponente beteiligt, wird bei geeigneten Reaktionsbedingungen zusätzlich mit Umsetzungen anderer im Proteinmolekül vorhandener funktioneller Gruppen zu rechnen sein. An dem erstmals von Freimuth und Trübsbach angewandten System β‐Lactoglobulin‐Lactose kann gezeigt werden, daß Thiol‐ und Disulfidgruppen bei hoher Temperatur in wasserarmem Milieu die Beschaffenheit der Maillard‐Reaktionsprodukte beeinflussen. In Abhängigkeit von der An‐ oder Abwesenheit dieser reaktiven Gruppen verändern sich Menge, Löslichkeit bzw. Quellbarkeit und die Zusammensetzung.
Zinnwirkung auf Ratten. 1. Mitt. Die inversvoltammetrische Bestimmung von Zinn neben Blei in biologischem Material nach Extraktion
Authors: Woggon H. , Raab M. , Jehle D. 
Published: November 2006

AbstractNach doppelter Extraktion mit Diäthylammoniumdiäthyldithiocarbamat (DADDTC) in perchlorsaurer Lösung wird das Zinn selektiv inverspolarographisch bestimmt. Der Mittelwert der Wiederauffindung beträgt bei Kartoffeln 91,7 ± 5,8%, Rinderniere 85,0 ± 22,7%, Rinderleber 79,9 ± 13,8% und Milch 69,8 ± 11,4%. Eine eventuelle Störung der Zinn‐Bestimmung durch andere Elemente wird diskutiert.
The role of sulphur compounds in evaluation of flavouring value of some plant raw materials
Authors: Tokarska B. , Karwowska K. 
Published: November 2006

AbstractThe aim of the study was to determine the composition of flavour isolates from garlic and horse‐radish, prepared by dichlorodifluoromethane extraction. Gas chromatography, with olfactory determination of the flavour of the resolved components, and gas chromatography combined with mass spectrometry were used. In the garlic extract 19 components comprising mono‐, di‐, and trisulphides and thiophene derivatives were detected. In the horse‐radish extract 14 components including isothiocyanates, thiocyanates and cyanides were identified.
Synthesis and analysis of l‐octen‐3‐ol, the main flavour component of mushrooms
Authors: Wnuk S. , Kinastowski S. , Kaminski E. 
Published: November 2006

AbstractThe most abundant volatile occurring in mushrooms and responsible for the mushroom odour is 1‐octen‐3‐ol. To meet the demand for a flavour compound yielding a mushroom odour a study was carried out on the possibility of obtaining l‐octen‐3‐ol synthetically. On the basis of literature data and experiments performed the synthesis of this compound was carried out by two methods, i.e. by GRIGNARD reaction between acrolein and amyl iodide and by selective reduction of l‐octen‐3‐on. The purity of the l‐octen‐3‐ol obtained was determined by GLC chromatography and by spectroscopic methods. The compound obtained by GRIGNARD reaction had its IR, 13C NMR spectra and GLC chromatogram identical with those of the standard. The yield of 1 ‐octen‐3‐ol by GRIGNARD reaction was 65 %, while the reduction of the ketone to the alcohol gave a yield of 90%.
The toxicity of the daily intake of nitrite and dimethylamine
Authors: Roché M. García , Castillo A. , Ballenilla T. , Silva V. , Cabrera Y. 
Published: November 2006

AbstractIt is known that sodium nitrite and dimethylamine are toxic compounds, which may react to form dimethyl‐nitrosamine in the gastro‐intestinal tract, a much more toxic compound and a powerful cancerogen. The aim of the present work is the investigation of toxicity in young rats, caused by daily intake of sodium nitrite, administered together with dimethylamine during 30 days. The indicators examined were: histopathological analysis of the liver and kidney, transaminase in blood serum, variations in body weight and relation of weight liver/body weight. The method of analysis used to determine transaminase was that reported by REINTMAN and FRANKEL in 1957. The statistical method employed was the test of multiple comparisons based on the total ranges of CRUSKAL‐WALLIS. The results show that either significant differences were found (α = 0,05) among the groups (including the control), nor was necrosis observed or forming of tumors in the organs under investigation. Therefore, the dosis administered does not seem to be toxic under the conditions of the experiment. Some signs of toxicity found in the group which was more severely treated (10 mg of sodium nitrite and 20 mg of dimethylamine) appeared only in a few animals and it is necessary to verify the same in experiments with more animals and over a longer period of treatment.
Untersuchungen zur Maillard‐Reaktion 11. Mitt. Derivatographische Untersuchungen am System Glucose/p‐Toluidin
Authors: Westphal G. , Örsi F. , Kroh L. , Bochow Ch. 
Published: November 2006

AbstractAus den derivatographischen Untersuchungen am System Glucose/p‐Toluidin läßt sich die solvensfreie Maillard‐Reaktion einem Temperaturbereich von 80–200£C zuordnen. Zwischen dem N‐(p‐Toluidino)‐glucopyranosid und der 1‐Desoxy‐1‐(p‐toluidino)‐fructopyranose, ersten Produkten dieser Reaktion, bestehen hinsichtlich ihres derivatographischen Verhaltens als auch ihrer Temperaturstabilität signifikante Unterschiede. Eine erstmals gefundene, thermisch induzierte Amadori‐Umlagerung ließ sich mit Hilfe von HPLC‐Untersuchungen der derivatographischen Proben nachweisen. Ausgehend von N‐(p‐Toluidino)‐glucopyranosid, konnte das Temperaturoptimum der Umlagerungsreaktion zu 130£C bestimmt werden.
Die quantitative Zusammensetzung der Lactoflora des Verdauungstrakts vor und nach kosmischen Flügen unterschiedlicher Dauer
Authors: Lencner A. A. , Lencner Ch. P. , Mikelsaar M. E. , Tjuri M. E. , Toom M. A. , Väljaots M. E. , Šilov V. M. , Liz'Ko N. N. , Legenkov V. I. , Reznikov I. M. 
Published: November 2006

AbstractIn einer Untersuchung der Lactoflora von 24 Kosmonauten wird gezeigt, daß in der Periode vor dem Start zum Raumflug ausgeprägte Veränderungen im Verdauungstrakt vorkommen, die den quantitativen Gehalt verschiedener Lactobacillus‐Arten betreffen können. Die Ursache hierfür ist im emotionellen Streß zu suchen. Nach Kurzzeitflügen werden sogar tiefer eingreifende Verschiebungen beobachtet als nach Langzeitflügen, da die vor dem Start eingetretenen Störungen infolge der kurzen Zeitdauer vom Organismus nicht ausgeglichen werden können. Zusätzlich beginnen die Faktoren des Raumflugs zu wirken, wobei die Adaptation eine bestimmte Zeit erfordert. Für die Veränderungen der Menge der Lactoflora im Speichel und Stuhl bei den Kosmonauten sind individuelle Besonderheiten charakteristisch.
Die quantitative Bestimmung des Proteingehaltes der Milch mittels Ultraviolettspektralphotometrie 2. Mitt. Zur Eignung der Differenz der Absorbanzwerte bei 235 und 280 nm für die quantitative Milchproteinanalytik
Authors: Reichardt W. , Schüler E. 
Published: November 2006

AbstractDer Proteingehalt von entfetteter Milch ist nach Verdünnung mit Alkylpolyglycoläthersulfatlösung mit Hilfe von Absorbanzmessungen bei 235 und 280 nm bestimmt worden. Die Differenz der Absorbanzwerte A235 ‐ A280 weist eine hohe Korrelation zu den Ergebnissen der Kjeldahl‐Stickstoffanalyse auf (r = 0,99). Der Proteingehalt von Caseinat‐ und Milchserumlösungen konnte analog ermittelt werden. Die Methode besitzt den Vorzug, von der Aminosäurenzusammensetzung des Proteins, dem Nucleinsäure‐, Salz‐, Lactose‐ und Nichtproteinstickstoffgehalt der Milch weitgehend unabhängig zu sein. Beide Absorbanzwerte sind aus Lösungen der gleichen Konzentration bestimmt worden. Es wurde die Abhängigkeit der Meßergebnisse vom Alter der Lösung. vom Alter der Milch und von der Temperatur untersucht sowie die Eignung von Konservierungsmitteln geprüft.
Regression analysis applied to evaluation of aroma and flavour of Bulgarian sour milk
Authors: Gyosheva B. 
Published: November 2006

AbstractThis investigation was carried out with the purpose of creating an objective criterion for evaluation of the aroma and flavour of Bulgarian sour milk (BSM) by mathematical and statistical elaboration of data obtained by chemical, gas chromatographical and bacteriological analysis of the product and thus complementing the sensory evaluation. Regression analysis was applied for the elaboration of data obtained by the analysis of 135 BSM samples, whereupon models were postulated for establishing a relationship between analysis data and sensory evaluation.The results of the performed tests on the adequacy of the models indicated that BSM aroma and flavour can be regarded to a great extent as a function of the total effect of the volatile aroma compounds, titratable acidity, and the ratio of S. thermophilus and L. bulgaricus.
Zum enzymatischen Abbau des DDT 4. Mitt. Abbau des DDD (Dichlordiphenyldichlorethan)
Authors: Kujawa M. , Macholz R. M. , Lewerenz H. J. 
Published: November 2006

AbstractDurch reduktive Dechlorierung entsteht DDD aus dem DDT. Nach Applikation von DDD an Ratten konnte das Dehydrochlorierungsprodukt DDMU nur nach kurzen Expositionszeiten in den Organen, in hohen Konzentrationen jedoch ständig im Kot nachgewiesen werden. Die Verteilung des DDD im Rattenorganismus wird ermittelt.
Basic protein isolate from lobster shell
Authors: Oviedo D. , García I. , Henriques R. D. 
Published: November 2006

AbstractSome structural characteristics of the basic protein concentrate isolated from the lobster shell were determined. The influence of the NaOH concentration, temperature and treatment time on the concentrate characteristics were also analyzed. An adequate combination of treatment time and alkaline concentration allowed to obtain similar protein yields with closer analogies in their structural characteristics, but the lower alteration and higher molecular weight are produced with the longest treatment time and the lowest alkaline concentration.
Untersuchungen zum Metabolismus von Fenazox in Tomatenpflanzen
Authors: Stock M. , Bernasch A. , Schütte H. R. 
Published: November 2006

AbstractDie in Versuchen zum Metabolismus von 14C‐Fenazox erzielten Ergebnisse zeigen, daß der Wirkstoff in 6–8 Wochen alten Tomatenpflanzen der Sorte „Harzfeuer”︁ einer Biotransformation unterliegt. Nach chromatographischer Trennung wurden die Biotransformationsprodukte durch Vergleich mit authentischen Testsubstanzen, durch Derivatisierung sowie mittels physikalisch‐chemischer Methoden in ihrer Struktur aufgeklärt. Dabei wurden folgende Metabolite identifiziert: Nicht umgesetztes Fenazox, o‐Hydroxyazobenzen, o‐Hydroxyanilin und p‐Hydroxyanilin. Wahrscheinlich wird der Wirkstoff zunächst enzymatisch oder nichtenzymatisch in o‐Hydroxyazobenzen umgewandelt, dann erfolgt eine reduktive Spaltung in o‐Hydroxyanilin und Anilin. Letzteres wird seinerseits in o‐Hydroxy‐ und p‐Hydroxyanilin umgewandelt. Experimente zur Umwandlungskinetik zeigen, daß das applizierte Fenazox in seiner Konzentration schnell abnimmt, daß das erste Umwandlungsprodukt o‐Hydroxyazobenzen anfangs ansteigt, um dann in seiner Konzentration wieder abzusinken. Mit zunehmender Applikationsdauer kommt es zu einem Anstieg der nichtextrahierbaren Rückstände.
Trypsin and chymotrypsin inhibitor activities in plant foods from Vietnam and Hungary
Authors: Vinh Le Tien , Dworschák E. 
Published: November 2006

AbstractA method for the determination of chymotrypsin inhibitor activity and a screening test for evaluating higher activities of trypsin inhibitor were elaborated.The protease inhibitor activities of the most important plant foods from Vietnam and Hungary were determined. In cereals and legume seeds the activity of chymotrypsin inhibitors is generally lower than that of the trypsin inhibitors. In various soy products (isolates, texturates) the extent of lowering of chymotrypsin inhibitor activity was less than that of trypsin inhibitor related to raw soy bean. The cooking process according to the usual kitchen technique of Vietnam has more effect on the higher trypsin inhibitor level of soy bean than on other legumes of average activity.