bluuebunny/crossref_metadata_2025_split huggingface dataset:
Energy sustainability and carbon dioxide emissions mitigation options for South Africa’s road transport sector
Authors: Ntuli Menzi Nazi , Eloka-Eboka Andrew C. , Mwangi Festus Maina , Ewim Daniel Raphael Ejike , Dioha Michael O. 
Published: April 2024

Abstract
Background
The transport sector in South Africa is responsible for around 11% of the country's carbon dioxide emissions, with road transport contributing an overwhelming 90% of this total, as noted by the South African Green Transport Energy of South Africa. As part of its commitment to global climate pacts, South Africa aims to reduce emissions from its road transport sector. Yet, studies focused on reducing energy consumption and related emissions in this sector have been sparse.

Results
Utilizing a bottom-up accounting modelling framework Low Emissions Analysis Platform (LEAP), this research investigated five low-carbon transition scenarios alongside a business-as-usual (BAU) scenario for road transport. These scenarios comprised Fuel and Technology Switching (FTS), Modal Shift (MS), Logistics Improvement (LI), Energy Efficient (EEF), and a Combined Mitigation (CMT). The BAU scenario was established as a benchmark to demonstrate energy demand and emissions in the absence of changes to current practices or policies. According to our model, under the BAU scenario, there will be a 61% surge in final energy demand, from 769 petajoules (PJ) in 2020 to 1240 PJ by 2050, accompanied by a proportional increase in emissions. The study revealed that the implementation of any of the alternative low-carbon scenarios could yield a reduced energy demand by 2050. LI 21%, MS 33%, FTS 40%, EEF 48%, CMT 77%, Significantly, a combined approach, integrating multiple low-carbon policies, can achieve more substantial reductions in energy demand and Carbon Dioxide (CO2) emissions than applying single policies separately.

Conclusions
This study emphasizes the importance of crafting province-specific solutions, acknowledging that challenges and contexts vary between provinces. Furthermore, lessening energy reliance not only diminishes the nation's fuel import bills but also improves air quality and aids in achieving low emission targets.
Dietary phytonutrients in common green leafy vegetables and the significant role of processing techniques on spinach: a review
Authors: Sarma Upasana , TR Bhavya 
Published: April 2024

AbstractLeafy vegetables comprises of the majority of food intake around the world. A nuanced understanding of their phytonutrients, bioavailability and post-harvest processing will aid in understanding their utility in human health better. Plant foods have a variety of dietary phytonutrients beneficial to us. With a lot of diversity in the variety of these leafy vegetables, it is of utmost importance as consumers to understand their benefits, functional properties, post processing changes that occurs until it reaches us. Some of the most popular green leafy vegetables include spinach, cabbage, lettuce and mustard greens. In this review, we provide a summary of the phytonutrients in such leafy greens with a detailed description of its bioavailability of nutrients, role of bio fortification, changes during harvest and post-harvest processing. As a low calorie food item, green leafy vegetables are ideal candidates to add valuable nutrients into our daily diets, and spinach especially is known to have multiple therapeutic implications in human health. Post-harvest processing may include addition of nutrients, increasing bio availability of important constituents, assessing effect of fertilizers and growth promoting factors on their nutrient content. All of these parameters need to be studied in depth to improve their beneficial effect in human nutrition and diet.
Graphical Abstract
Diet in Food Insecurity: A Mediator of Metabolic Health?
Authors: Morselli Lisa L , Amjad Rabia , James Roland , Kindel Tammy L , Kwitek Anne E , Williams Joni S , Grobe Justin L , Kidambi Srividya 
Published: April 2024

Abstract

Objective
Food insecurity (FI) is associated with poor metabolic health. It is assumed that energy intake and diet quality underlie this association. We tested the hypothesis that dietary factors (quantity and quality) mediate the association of FI with excess weight, waist circumference and glycemic control [glycohemoglobin (A1C)].

Methods
A mediation analysis was performed on data from the National Health And Nutrition Examination Survey using FI as an independent variable; body mass index (BMI), waist circumference, and A1C as metabolic outcome variables and total energy intake, macronutrients, and diet quality measured by the Healthy Eating Index-2015 (HEI-2015) as potential mediators.

Results
Despite a greater prevalence of obesity in participants experiencing FI, daily reported energy intake was similar in food-secure and -insecure subjects. In adjusted analyses of the overall cohort, none of the examined dietary factors mediated associations between FI and metabolic outcomes. In race-stratified analyses, total sugar consumption was a partial mediator of BMI in non-Hispanic Whites, while diet quality measures (HEI-2015 total score and added sugar subscore) were partial mediators of waist circumference and BMI, respectively, for those in the “other” ethnic group.

Conclusion
Dietary factors are not the main factors underlying the association of FI with metabolic health. Future studies should investigate whether other social determinants of health commonly present in the context of FI play a role in this association.
Purkinje cell dysfunction causes disrupted sleep in ataxic mice
Authors: Salazar Leon Luis E. , Brown Amanda M. , Kaku Heet , Sillitoe Roy V. 
Published: April 2024

ABSTRACT
Purkinje cell dysfunction disrupts movement and causes disorders such as ataxia. Recent evidence suggests that Purkinje cell dysfunction may also alter sleep regulation. Here, we used an ataxic mouse model generated by silencing Purkinje cell neurotransmission (L7Cre;Vgatfx/fx) to better understand how cerebellar dysfunction impacts sleep physiology. We focused our analysis on sleep architecture and electrocorticography (ECoG) patterns based on their relevance to extracting physiological measurements during sleep. We found that circadian activity was unaltered in the mutant mice, although their sleep parameters and ECoG patterns were modified. The L7Cre;Vgatfx/fx mutant mice had decreased wakefulness and rapid eye movement (REM) sleep, whereas non-REM sleep was increased. The mutants had an extended latency to REM sleep, which is also observed in human patients with ataxia. Spectral analysis of ECoG signals revealed alterations in the power distribution across different frequency bands defining sleep. Therefore, Purkinje cell dysfunction may influence wakefulness and equilibrium of distinct sleep stages in ataxia. Our findings posit a connection between cerebellar dysfunction and disrupted sleep and underscore the importance of examining cerebellar circuit function in sleep disorders.
Synthesis, characterization and preliminary antimicrobial study of some new phthalimide phenyl hydrazide derivatives
Authors: Alassadi Nisreen Mahdi , Hadi Mohammed Kamil 
Published: April 2024

Background: The synthesis and antibacterial efficacy of phthalimide Schiff bases derivatives (3a-f) derived from phthalic anhydride were investigated. The aim of this study was to assess the antibacterial activity of the synthesized Schiff bases against five different types of microbes in vitro. Methods: The Schiff bases were synthesized by reacting various aldehydes with the hydrazine of phthalimidobenzoic acid. The compounds were identified using FTIR and 1HNMR spectroscopy studies, and their antibacterial efficacy against four bacterial species (Staphylococcus aureus, Streptococcus pyrogens, Escherichia coli, and Pseudomonas aeruginosa) and one fungal species (Candida albicans) was assessed in vitro using a 100 µg/mL concentration of derivatives in dimethyl sulfoxide. Results: The antimicrobial activity of each compound towards the isolates was variable. Most of the compounds exhibited a slight inhibition rate towards Gram-positive and Gram-negative bacteria, particularly S. aureus, which was shown to be highly resistant to all derivatives used. However, there was moderate to high killing activity towards S. pyogenes. Conclusions: The synthesized phthalimide Schiff bases derivatives exhibited variable antibacterial activity against the tested microbes. The results suggest that further studies are required to optimize the efficacy of these compounds against harmful microorganisms.
Impact of transcranial electrical stimulation on serum neurotrophic factors and language function in patients with speech disorders
Authors: Sun Li , Xiao Kai , Shen Xiao-Yan , Wang Shu 
Published: April 2024

BACKGROUND
Speech disorders have a substantial impact on communication abilities and quality of life. Traditional treatments such as speech and psychological therapies frequently demonstrate limited effectiveness and patient compliance. Transcranial electrical stimulation (TES) has emerged as a promising non-invasive treatment to improve neurological functions. However, its effectiveness in enhancing language functions and serum neurofactor levels in individuals with speech disorders requires further investigation.
AIM
To investigate the impact of TES in conjunction with standard therapies on serum neurotrophic factor levels and language function in patients with speech disorders.
METHODS
In a controlled study spanning from March 2019 to November 2021, 81 patients with speech disorders were divided into a control group (n = 40) receiving standard speech stimulation and psychological intervention, and an observation group (n = 41) receiving additional TES. The study assessed serum levels of ciliary neurotrophic factor (CNTF), glial cell-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF), as well as evaluations of motor function, language function, and development quotient scores.
RESULTS
After 3 wk of intervention, the observation group exhibited significantly higher serum levels of CNTF, GDNF, BDNF, and NGF compared to the control group. Moreover, improvements were noted in motor function, cognitive function, language skills, physical abilities, and overall development quotient scores. It is worth mentioning that the observation group also displayed superior performance in language-specific tasks such as writing, reading comprehension, retelling, and fluency.
CONCLUSION
This retrospective study concluded that TES combined with traditional speech and psychotherapy can effectively increase the levels of neurokines in the blood and enhance language function in patients with speech disorders. These results provide a promising avenue for integrating TES into standard treatment methods for speech disorders.
Fast and high-accuracy collinear reflection Mueller imaging polarimeter implemented with the compound calibration method
Authors: Ma Xiang , Wu Jinxian , Hu Yujie , Li Yanqiu 
Published: April 2024

The collinear reflection Mueller matrix imaging polarimeter is suitable for characterizing thick samples with high-scattering depolarization such as biological tissues or in-situ living organs. Achieving fast detection and high measurement accuracy is vital to prevent artifacts and accurately assess polarization characteristics in these applications. This paper demonstrates a fast collinear reflection imaging polarimeter based on liquid crystal variable retarders (LCVRs-CRMMIP). We propose a novel compound calibration method (CCM), to the best of our knowledge, which enhances measurement accuracy through light intensity correction and an improved equivalent calibration sample model. This method surpasses the double-pass eigenvalue calibration method (dp-ECM), enhancing accuracy by over 23 times. Performance evaluations with standard samples, including mirrors, linear polarizers, and wave plates, reveal that the LCVRs-CRMMIP achieves rapid measurements (about 3 s) and high accuracy with an error of less than 0.0017.
Impact of Strongyloides stercoralis infection on complement activation in Type 2 diabetes mellitus: Insights from a clinical and anthelmintic intervention study
Authors: Rajamanickam Anuradha , Dasan Bindu , Munisankar Saravanan , Nott Sujatha , Menon Pradeep A. , Ahamed Shaik Fayaz , Chinnaiyan Ponnuraja , Nutman Thomas B. , Babu Subash 
Published: April 2024

Background
Numerous studies indicate a potential protective role of helminths in diabetes mellitus (DM) progression. The complement system, vital for host defense, plays a crucial role in tissue homeostasis and immune surveillance. Dysregulated complement activation is implicated in diabetic complications. We aimed to investigate the influence of the helminth, Strongyloides stercoralis (Ss) on complement activation in individuals with type 2 DM (T2D).

Methodology
We assessed circulating levels of complement proteins (C1q, C2, C3, C4, C4b, C5, C5a, and MBL (Lectin)) and their regulatory components (Factor B, Factor D, Factor H, and Factor I) in individuals with T2D with (n = 60) or without concomitant Ss infection (n = 58). Additionally, we evaluated the impact of anthelmintic therapy on these parameters after 6 months in Ss-infected individuals (n = 60).

Results
Ss+DM+ individuals demonstrated reduced levels of complement proteins (C1q, C4b, MBL (Lectin), C3, C5a, and C3b/iC3b) and complement regulatory proteins (Factor B and Factor D) compared to Ss-DM+ individuals. Following anthelmintic therapy, there was a partial reversal of these levels in Ss+DM+ individuals.

Conclusion
Our findings indicate that Ss infection reduces complement activation, potentially mitigating inflammatory processes in individuals with T2D. The study underscores the complex interplay between helminth infections, complement regulation, and diabetes mellitus, offering insights into potential therapeutic avenues.
Interruptions in a dental setting and evaluating the efficacy of an intervention: A pilot study
Authors: Ziegler Carsten , Parikh Pratik J. 
Published: April 2024

Introduction
Interruptions during dental treatment are frequent, and often impact provider satisfaction and processing times We investigate the source and duration of such interruptions at a German dental clinic.

Methods
A pre-post approach was adopted at this dental clinic. This included direct observations of 3 dentists and 3 dental hygienists, and a survey of providers. Following that, an intervention (switchable ‘Do Not Enter’ sign) was chosen, and a pilot study was conducted to evaluate if the chosen intervention can reduce processing time and improve provider satisfaction. Additional observations and surveys were performed afterwards.

Results
Pre-intervention data indicated that interruptions have the highest negative impact on provider satisfaction at this clinic as well as on processing time during longer and more complex treatments, where a minor error due to an interruption could lead to rework of 30 minutes and more. The total number of interruptions dropped by 72.5% after the intervention, short interruptions (< 1min) by 86%. Provider survey indicated improvement due to the intervention in perceived workload, provider work satisfaction, patient safety and stress.

Conclusions
This study demonstrates that a switchable sign can substantially reduce the number of interruptions in this dental clinic. It also shows the potential of improving the work environment by reducing interruptions to the dental providers.
Association between the COVID-19 pandemic and mental health in very old people in Sweden
Authors: Jonsson Fanny , Olofsson Birgitta , Söderberg Stefan , Niklasson Johan 
Published: April 2024

Background
During the COVID-19 pandemic, Sweden implemented social distancing measures to reduce infection rates. However, the recommendation meant to protect individuals particularly at risk may have had negative consequences. The aim of this study was to investigate the impact of the COVID-19 pandemic on very old Swedish peoples’ mental health and factors associated with a decline in mental health.

Methods
We conducted a cross-sectional study among previous participants of the SilverMONICA (MONItoring of Trends and Determinants of CArdiovascular disease) study. Of 394 eligible participants, 257 (65.2%) agreed to participate. Of these, 250 individuals reported mental health impact from COVID-19. Structured telephone interviews were carried out during the spring of 2021. Data were analysed using the χ2 test, t-test, and binary logistic regression.

Results
Of 250 individuals (mean age: 85.5 ± 3.3 years, 54.0% women), 75 (30.0%) reported a negative impact on mental health, while 175 (70.0%) reported either a positive impact (n = 4) or no impact at all (n = 171). In the binary logistic regression model, factors associated with a decline in mental health included loneliness (odds ratio [95% confidence interval]) (3.87 [1.83–8.17]) and difficulty adhering to social distancing recommendations (5.10 [1.92–13.53]). High morale was associated with positive or no impact on mental health (0.37 [0.17–0.82]).

Conclusions
A high percentage of very old people reported a negative impact on mental health from the COVID-19 pandemic, primarily from loneliness and difficulty adhering to social distancing measures, while high morale seemed to be a protective factor.
Sex-structured disease transmission model and control mechanisms for visceral leishmaniasis (VL)
Authors: Awoke Temesgen Debas , Kassa Semu Mitiku , Morupisi Kgomotso Suzan , Tsidu Gizaw Mengistu 
Published: April 2024

BackgroundLeishmaniasis are a group of diseases caused by more than 20 species of the protozoan that are transmitted through the bite of female sand fly. The disease is endemic to 98 countries of the world. It affects most commonly the poorest of the poor and mainly males. Several research has been conducted to propose disease control strategies. Effective medical care, vector control, environmental hygiene, and personal protection are the mainstays of the current preventative and control methods. The mathematical models for the transmission dynamics of the disease studied so far did not consider the sex-biased burden of the disease into consideration.MethodologyUnlike the previous VL works, this study introduces a new deterministic sex-structured model for understanding the transmission dynamics of visceral leishmaniasis. Basic properties of the model including basic reproduction number (R0), and conditions for the existence of backward bifurcation of the model are explored. Baseline parameter values were estimated after the model was fitted to Ethiopia’s VL data. Sensitivity analysis of the model was performed to identify the parameters that significantly impact the disease threshold. Numerical simulations were performed using baseline parameter values, and scenario analysis is performed by changing some of these parameters as appropriate.ConclusionThe analysis of the model shows that there is a possibility for a backward bifurcation forR0<1, which means bringingR0to less than unity may not be enough to eradicate the disease. Our numerical result shows that the implementation of disease-preventive strategies, as well as effectively treating the affected ones can significantly reduce the disease prevalence if applied for more proportion of the male population. Furthermore, the implementation of vector management strategies also can considerably reduce the total prevalence of the disease. However, it is demonstrated that putting more effort in treating affected reservoir animals may not have any significant effect on the overall prevalence of the disease as compared to other possible mechanisms. The numerical simulation infers that a maximum of 60% of extra preventative measures targeted to only male population considerably reduces the total prevalence of VL by 80%. It is also possible to decrease the total prevalence of VL by 69.51% when up to 50% additional infected males receive treatment with full efficacy. Moreover, applying a maximum of 15% additional effort to reduce the number of vectors, decreases the total VL prevalence by 57.71%. Therefore, in order to reduce the disease burden of visceral leishmaniasis, public health officials and concerned stakeholders need to give more emphasis to the proportion of male humans in their intervention strategies.
Understanding the limitations of your assay using EQA data with serum creatinine as an example
Authors: Marrington Rachel , MacKenzie Finlay 
Published: April 2024

Abstract

Objectives
Laboratories need to take into consideration the specificity and imprecision of assays not only in verification, but also of quality assessment. This study investigates the composition of serum used in EQA materials by comparing material from a single and multiple donors (pooled material), across multiple methods, using creatinine as an example.

Methods
Sixteen different serum matrices were distributed as 36 specimens through the UK NEQAS for Acute and Chronic Kidney Disease Scheme from March 2022 to March 2023. Male-only and female-only serum was used as single donations, pooled donations, unmanipulated or with added exogenous creatinine. Specimens were distributed to primarily UK participants (approximately n=500) for creatinine analysis. Data has been reviewed by method compared to the enzymatic creatinine method principle mean.

Results
From the 16 different matrices, only the enzymatic creatinine assay systems from Roche Cobas and Siemens Atellica met the minimum acceptable bias goal, from biological data, of 5.6 %, in all specimens. Pooled material showed less variation in bias across all methods.

Conclusions
Since Laboratories invest a lot of time and money in quality management, they need to know the limitations of their assays so that they are not investigating ‘apparent’ EQA/IQC problems which are purely due to non-specific, imprecise assay, rather than an analytical issue in their laboratory. When large numbers of individual donations are combined, interferents are essentially diluted out. Therefore, if EQA material is of this type it will be very difficult to determine the actual assay’s bias and variability.
Performance Evaluation of Economic Relocation Effect for Environmental Non-Governmental Organizations: Evidence from China
Authors: Li Guangqin , Luo Ji , Liu Siyan 
Published: April 2024

AbstractIn this article, an economic natural experiment in China is analyzed and the performance evaluation of the economic relocation effect of informal environmental regulations imposed through the channel of environmental nongovernmental organizations (ENGOs) is suggested. ENGOs are found to have a significantly negative impact on the overall economic location quotient of six major polluting industries. The economic relocation effect from ENGOs exhibits industrial and regional heterogeneity, as the effect is stronger among easy-to-relocate industries and in market-oriented areas. The underlying mechanism is the ENGOs, as informal environmental regulations could motivate firms to enhance their investment in environmental governance, ultimately crowding out these economically polluting industries.