bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contact urticaria from protein hydrolysates in hair conditioners
Authors: Niinimäki A. , Niinimäki M. , Mäkinen‐Kiljunen S. , Hannuksela M. 
Published: May 2007

Protein hydrolysates (PHs) are added to hair‐care products (to “repair” broken hair), soaps, bath gels, creams, etc. From one to 22 PHs used in hair‐care products (collagen, keratin, elastin, milk, wheat, almond, and silk) were tested in three patient groups: A) 11 hairdressers with hand dermatitis B) 2160 consecutive adults with suspected allergic respiratory disease subjected to routine skin prick tests C) 28 adults with atopic dermatitis.In group A, all the 22 PHs were tested with scratch and patch tests. In groups B and C, one to three PHs were tested with prick tests. Positive scratch/prick test reactions were seen in 12 patients from three PHs altogether. All were women with atopic dermatitis, and all reacted to at least hydroxypropyl trimonium hydroly:ed collagen (Crotein Q”). In three patients, prick and open tests with a hair conditioner containing Crotein Q were performed with positive results. One patient reported contact urticaria on her hands, and two reported acute urticaria on their head, face, and upper body from a hair conditioner containing Crotein Q. In seven of the eight studied sera, specific IgE to Crotein Q was detected. In conclusion, PHs of hair cosmetics can cause contact urticaria, especially in patients with atopic dermatitis.
Allorecognition and the alloresponse: clinical implications
Authors: Afzali B. , Lechler R. I. , Hernandez‐Fuentes M. P. 
Published: May 2007

AbstractThe artificial transfer of tissues or cells between genetically diverse individuals elicits an immune response that is adaptive and specific. This response is orchestrated by T lymphocytes that are recognizing, amongst others, major histocompatibility complex (MHC) molecules expressed on the surface of the transferred cells. Three pathways of recognition are described: direct, indirect and semi‐direct. The sets of antigens that are recognized in this setting are also discussed, namely, MHC protein products, the MHC class I‐related chain (MIC) system, minor histocompatibility antigens and natural killer cell receptor ligands. The end product of the effector responses are hyperacute, acute and chronic rejection. Special circumstances surround the situation of pregnancy and bone marrow transplantation because in the latter, the transferred cells are the ones originating the immune response, not the host. As the understanding of these processes improves, the ability to generate clinically viable immunotherapies will increase.
The genetic variation of the human HMGB1 gene
Authors: Kornblit B. , Munthe‐Fog L. , Petersen S. L. , Madsen H. O. , Vindeløv L. , Garred P. 
Published: May 2007

AbstractHigh‐mobility group box 1 protein (HMGB1) is a nuclear DNA‐binding protein, which also functions as a pleiotropic cytokine, implicated in the pathology of several different immune‐mediated diseases. The purpose of this study was to examine the HMGB1 gene for putative polymorphisms in 103 healthy Caucasian Danish blood donors. A total of six polymorphisms and four mutations were identified in the HMGB1 gene. Subsequent MatInspector estimation revealed that several polymorphisms might have a potential regulatory impact on HMGB1 transcription. This study has characterized genetic variations in the HMGB1 gene locus, which may have a regulating role in the expression of HMGB1, providing the basis for molecular investigations of the HMGB1 gene in different disease settings.
HLA‐G alleles and HLA‐G 14 bp polymorphisms in a Brazilian population
Authors: Castelli E. C. , Mendes‐Junior C. T. , Donadi E. A. 
Published: May 2007

AbstractBrazilians represent one of the most heterogeneous populations in the world as the result of five centuries of interethnic crosses. We investigated the polymorphism of the HLA‐G locus in a Brazilian urban sample. HLA‐G alleles were determined by SBT analysis of exons 2–4 and 8 in 103 unrelated individuals. The genotyping analysis identified 11 different HLA‐G alleles, including the recently described G*010110 allele, and four new variants in this population. These data indicate that this Brazilian population presents high HLA‐G allelic polymorphism, suggesting increased levels of intrapopulational genetic diversity that may be because of the admixed nature of the sample. This is the first study regarding HLA‐G genotyping that was performed at such refined resolution level in this heterogeneous population.
Evidence for disruption in prefrontal cortical functions in juvenile bipolar disorder
Authors: Bearden Carrie E , Glahn David C , Caetano Sheila , Olvera Rene L , Fonseca Manoela , Najt Pablo , Hunter Kristina , Pliszka Steve R , Soares Jair C 
Published: May 2007

Objectives:  Systematic parsing of executive function processes is critical for the development of more specific models of neurobiological processes mediating disturbed cognition in youth with bipolar disorder (BPD).Methods:  A sample of 33 children and adolescents with bipolar I disorder (BPD I) (mean age 12.1 ± 3.0 years, 39% female) and 44 demographically matched healthy participants (mean age 12.9 ± 2.8 years, 50% female) completed a neurocognitive battery including measures aimed at detection of disruption in prefrontal cortical circuitry (i.e., working memory, set shifting, and rule attainment).Results:  Compared to healthy controls, BPD I children exhibited significant deficits in spatial working memory, visual sequencing and scanning, verbal fluency and abstract problem solving, particularly when a memory component was involved. In our spatial delayed response task, memory set size was parametrically varied; the performance pattern in BPD I children suggested deficits in short‐term memory encoding and/or storage, rather than capacity limitations in spatial working memory. Earlier age at onset of illness and antipsychotic medication usage were associated with poorer performance on speeded information‐processing tasks; however, severity of mood symptomatology and comorbidity with disruptive behavior disorders were not associated with task performance.Conclusions:  These results suggest impairment in measures of prefrontal cortical function in juvenile BPD I that are similar to those seen in the adult form of the illness, and implicate both the ventral and dorsolateral prefrontal cortex as loci of pathology in juvenile BPD. As these deficits were not associated with clinical state or comorbidity with other disorders, they may reflect trait‐related impairments, a hypothesis that will be pursued further in longitudinal studies.
Abnormal cellular energy and phospholipid metabolism in the left dorsolateral prefrontal cortex of medication‐free individuals with bipolar disorder: an in vivo1H MRS study
Authors: Frey Benício N , Stanley Jeffrey A , Nery Fabiano G , Serap Monkul E , Nicoletti Mark A , Chen Hua‐Hsuan , Hatch John P , Caetano Sheila C , Ortiz Oswaldo , Kapczinski Flávio , Soares Jair C 
Published: May 2007

Objectives:  While the pathophysiology of bipolar disorder (BD) remains to be elucidated, postmortem and neuroimaging studies have suggested that abnormalities in the dorsolateral prefrontal cortex (DLPFC) are implicated. We compared the levels of specific brain chemicals of interest measured with proton magnetic resonance spectroscopy (1H MRS) in medication‐free BD subjects and age‐ and gender‐matched healthy controls. We hypothesized that BD subjects would present abnormal cellular metabolism within the DLPFC, as reflected by lower N‐acetyl‐aspartate (NAA) and creatine + phosphocreatine (Cr + PCr).Methods:  Thirty‐two medication‐free BD subjects (33.8 ± 10.2 years) and 32 matched controls (33.8 ± 9.0 years) underwent a short echo‐time (TE = 30 ms) 1H MRS. An 8‐cm3 single voxel was placed in the left DLPFC, and individual concentrations of NAA, Cr + PCr, choline‐containing compounds (GPC + PC), myo‐inositol, and glutamate were obtained, using the water signal as an internal reference.Results:  BD subjects had lower Cr + PCr [F(1,62) = 5.85; p = 0.018; one‐way analysis of variance (ANOVA)] and lower GPC + PC [F(1,62) = 5.79; p = 0.019; one‐way ANOVA] levels in the left DLPFC. No significant differences were observed for other brain metabolites.Conclusions:  These findings provide further evidence that the pathophysiology of BD involves impairment in the DLPFC. Our findings can be interpreted as evidence for reduced cellular energy and phospholipid metabolism, consistent with the hypothesis of mitochondrial dysfunction in BD.
Zygomycosis – a case report and overview of the disease in India
Authors: Diwakar Amit , Dewan R. K. , Chowdhary Anuradha , Randhawa H. S. , Khanna Geetika , Gaur S. N. 
Published: May 2007

SummaryA case of zygomycosis caused by Rhizopus oryzae in a diabetic patient previously misdiagnosed as invasive pulmonary aspergillosis and an overview of the disease in India are presented. The case was diagnosed by direct microscopy, histopathologic examination and culture. Following surgical resection of pulmonary cavity under cover of amphotericin B administration, the patient recovered completely. Of 461 cases reported to‐date, approximately 70% had been diagnosed at the Postgraduate Institute of Medical Education and Research, Chandigarh, in north India. This may be attributed to better awareness, expertise and infrastructural facilities for mycological diagnosis than to any particular regional preponderance of the disease. Rhino‐orbito‐cerebral manifestations were the most common feature of zygomycosis (269 cases), followed by cutaneous disease (66 cases), which is in conformity with the pattern prevalent worldwide. The etiologic agents encountered were Rhizopus oryzae, Apophysomyces elegans, Saksenaea vasiformis, Cunninghamella bertholletiae, Absidia corymbifera, Basidiobolus ranarum and Conidiobolus coronatus. In contrast to cases from the developed world where transplant recipients and patients with haematological malignancies seem to be most vulnerable to zygomycosis, the most common risk factor in India was uncontrolled diabetes mellitus. Amphotericin B was the mainstay of various treatment modalities employed. The relevance of a strong clinical suspicion and early diagnosis of zygomycosis for favourable prognosis can hardly be over‐emphasised.
Scedosporium apiospermum infection after near‐drowning
Authors: Katragkou Aspasia , Dotis John , Kotsiou Maria , Tamiolaki Maria , Roilides Emmanuel 
Published: May 2007

Summary Scedosporium apiospermum and its teleomorph (sexual form) Pseudallescheria boydii are ubiquitous saprophytic fungi, which under specific conditions, such as near‐drowning, may cause therapy‐refractory and life‐threatening infections. We reviewed 22 cases (eight children and 14 adults) of S. apiospermum infection after near‐drowning reported in the literature including an additional paediatric case from our institution. Scedosporiosis after near‐drowning was associated with high mortality (16/23, 70%) even in immunocompetent hosts. It affected mainly young (mean age 24 years) and immunocompetent (83% with no apparent immune defect) males (male to female ratio 2.5 : 1). Scedosporiosis after near‐drowning was a slow progressive disease (mean survival time 87 days) involving virtually all body organs. However, central nervous system (CNS) dissemination predominated (21/23, 91%) presenting mainly as multiple brain abscesses (15/23, 65%). All 23 patients showed preceding clinical and/or radiological evidence of lung disease indicating the mode of invasion. Diagnosis was delayed (median time to diagnosis 28 days) and was made by culture (16/23, 69.5%) or culture and tissue examination (7/23, 30.5%). The majority of the patients (20/23, 87%) received antifungal treatment and underwent neurosurgery. While the optimal treatment remains undefined, the most recent reports indicated voriconazole as a potentially effective option. Better knowledge of scedosporiosis after near‐drowning could lead to improved intervention and ultimately to more favourable outcome.
Wheat EST‐SSRs for tracing chromosome segments from a wide range of grass species
Authors: Zhang L. Y. , Bernard M. , Ravel C. , Balfourier F. , Leroy P. , Feuillet C. , Sourdille P. 
Published: May 2007

AbstractTransferability of 116 common wheat expressed sequence tag–simple sequence repeats (EST‐SSR) markers was investigated on 168 accessions representing 18 grass species to identify new alleles useful for wheat improvement. Transferability among the Triticeae ranged from 73.7% for Aegilops longissima to 100% for wheat subspecies (Triticum compactum) but was also good for less related species such as rye (72.8%) or maize (40.4%). On average, the number of alleles/locus detected by EST‐SSR markers was 3.1 for hexaploid wheat. The polymorphism information content (PIC) values simultaneously estimated for Triticum aestivum and Triticum durum were similar for the two species (0.40 and 0.39, respectively). The allelic diversity within allogamous species was higher (0.352–0.423) compared with that of T. aestivum and T. durum (0.108 and 0.093, respectively). T. aestivum and T. durum shared the largest number of alleles (74.6%) while among the three ancestral diploid species of bread wheat, Aegilops tauschii had the highest percentage of alleles with T. aestivum (57.4%). These results indicate that grass orphan species can be studied using wheat EST‐SSRs and can serve as a source of new alleles for wheat genetic improvement.
Simultaneous selection for early maturity, increased grain yield and elevated grain protein content in spring wheat
Authors: Iqbal M. , Navabi A. , Salmon D. F. , Yang R.‐C. , Spaner D. 
Published: May 2007

AbstractHigh grain yield and grain protein content, and early maturity are important traits in global bread wheat (Triticum aestivum L.)‐breeding programmes. Improving these three traits simultaneously is difficult due to the negative association between grain yield and grain protein content and the positive association between maturity and grain yield. We investigated the genetic relationship between maturity, grain yield and grain protein content in a population of 130 early maturing spring wheat lines in a high latitude (52–53°N) wheat‐growing region of Canada. Grain protein content exhibited negative genetic correlation with maturity (−0.87), grain fill duration (−0.78), grain fill rate (−0.49), grain yield (−0.93) and harvest index (−0.71). Grain yield exhibited positive genetic correlation with maturity (0.69), rate (0.78) and duration (0.49) of grain fill, and harvest index (0.55). Despite the positive association between maturity and grain yield, and negative association between grain yield and grain protein content, higher yielding lines with medium maturity and higher grain protein content were identified. Broad‐sense heritabilities were low (<0.40) for rate and duration of grain fill, grain protein content, spike per m2, grains per spike, harvest index and grain yield, and medium to high (>0.40) for grain weight, days to anthesis and maturity, and plant height. Selection for longer preanthesis and shorter grain fill periods may help circumvent the negative association between grain yield and grain protein content. Selection for shorter grain fill periods and higher grain fill rate may be a useful strategy for developing early maturing cultivars with acceptable grain yields in northern wheat‐growing regions.
A CAPS marker TAO1902 diagnostic for the I‐2 gene conferring resistance to Fusarium oxysporum f. sp. lycopersici race 2 in tomato
Authors: Staniaszek M. , Kozik E. U. , Marczewski W. 
Published: May 2007

Abstract Fusarium oxysporum f. sp. lycopersici inhabits most tomato‐growing regions worldwide, causing tomato production yield losses. A molecular marker linked to resistance would be useful for tomato improvement programmes. Thus, a cleaved amplified polymorphic sequence (CAPS) marker TAO1902 was developed to identify tomato genotypes possessing the I‐2 gene, which confers resistance to F. o. lycopersici race 2. The RsaI or FokI restriction fragments corresponded to the presence or absence of the I‐2 allele in a segregating 100 F2 progeny, tomato cultivars, 16 resistant and 20 susceptible to Fusarium wilt, respectively, lines and F1 hybrids, representing various tomato gene pools. TAO1902 may be helpful for selection of F. o. lycopersici‐resistant tomato germplasm.
Epigallocatechin‐3‐gallate and bicalutamide cause growth arrest and apoptosis in NRP‐152 and NRP‐154 prostate epithelial cells
Authors: Morrissey Colm , Brown Mark , O'Sullivan Jacintha , Weathered Natalie , Watson R. William G. , Tenniswood Martin 
Published: May 2007

Aim:  A number of epidemiological studies have suggested that consumption of green tea reduces the risk of prostate cancer. The aim of this study was to elucidate the effects of epigallocatechin‐3‐gallate (EGCG), one of the major constituents of green tea, on growth inhibition and apoptosis in prostate epithelial cell lines with and without bicalutamide.Methods:  The effects of EGCG and bicalutamide alone and in combination were examined on NRP‐152 and NRP‐154 cells derived from the dorso‐lateral prostate of the Lobund‐Wistar rat. Following treatments, cell number and levels of apoptosis were assessed.Results:  After treatment with EGCG, both cell lines displayed a dose‐dependent decrease in cell number; this effect was more pronounced in NRP‐154 cells. This decrease in cell number was caused by growth arrest in NRP‐152 cells and apoptosis in NRP‐154 cells. The apoptotic events in the NRP‐154 cells were concurrent with a loss of manganese superoxide dismutase expression. Androgen ablation was achieved by androgen withdrawal using charcoal stripped serum or treatment with bicalutamide. Bicalutamide decreased cell number and induced apoptosis in a dose‐dependent manner in both cell lines; however, androgen withdrawal did not. There was a loss of androgen receptor expression in NRP‐152 cells with bicalutamide treatment. However, as the NRP‐154 cells are androgen receptor negative, the loss in cell number and increased apoptotic events in these cells cannot be attributed to the anti‐androgenic activity of bicalutamide. Cells treated with a combination of bicalutamide and EGCG also demonstrated a dose‐dependent decrease in cell number that was significantly greater than bicalutamide alone.Conclusions:  This study demonstrates the potential use of EGCG and other antioxidants as therapeutic candidates for prostate cancer.
PERIPROSTHETIC FEMORAL FRACTURES AFTER TOTAL HIP ARTHROPLASTY
Authors: Young Simon W. , Pandit Salil , Munro Jacob T. , Pitto Rocco P. 
Published: May 2007

Background:  The management of periprosthetic fracture following a total hip arthroplasty is difficult, requiring expertise in both trauma and revision surgery. With rising numbers of patients in the population living with hip prostheses in situ, the frequency of these fractures is increasing, and controversy remains over their ideal management. The objective of this study was to review all periprosthetic fractures at a single institution to identify injury and treatment patterns and their associated clinical outcomes.Methods:  Fifty‐four periprosthetic fractures in 50 patients were reviewed to determine the relative frequency of fracture types, their complication rates and the clinical outcomes. Patient data were obtained through review of the clinical notes and individual patient follow up. Clinical outcomes were evaluated using the Oxford Hip Score and Harris Hip Score.Results:  The 54 fractures were classified using the Vancouver system, most of which were type B1 (20) or type B2 (10). The mean time to union for all fracture types was 4.6 months. A high non‐union rate was seen among fractures fixed operatively. Fifteen per cent of fractures went on to develop loosening following treatment, suggesting an underrecognition at the time of injury. The average Harris Hip Score was 73.1 and Oxford Hip Score 30.3 for all fracture types at a mean follow up of 3.3 years. In the 15 patients treated with revision surgery, the most common complication was dislocation (27%).Conclusion:  Treatment of patients with periprosthetic fractures requires recognition of the challenging nature of these injuries, the associated poor prognosis and the high complication rate.
PREOPERATIVE BETA‐BLOCKER USE REDUCES ATRIAL FIBRILLATION IN OFF‐PUMP CORONARY BYPASS SURGERY
Authors: Imren Yildirim , Benson Ariel A. , Zor Hakan , Tasoglu Irfan , Ereren Emrah , Sinci Volkan , Gokgoz Levent , Halit Velit 
Published: May 2007

Background:  Atrial fibrillation (AF) after coronary artery bypass graft surgery constitutes the most common sustained arrhythmia and results in many complications. The purpose of this study was to assess the effects of prophylactic use of beta‐blockers against atrial fibrillation in off‐pump surgery patients in the early postoperative period.Methods:  From 2002 to 2005, 78 patients were enrolled and 41 patients received 50 mg metoprolol succinate daily, which was initiated minimum four days before surgery. Preoperative beta‐blocking therapy was continued until the morning of surgery. Thirty‐seven patients were free of beta‐blocker therapy. Esmolol was used within same range of doses in both groups during operations. Both groups recieved metoprolol succinate following operations. The frequency of AF occurrence was analysed from the operation time to the sixth postoperative day.Results:  Sixteen patients developed AF with an overall incidence of 22.5%. Four patients from the study group and three patients from the control group were excluded from the study because of transfer to on‐pump surgery. There was no difference with regard to the number of grafts carried out, duration of operations and ventilation, intensive care unit stay and inotropic need among groups. Length of hospital stay did not differ among groups either. There was a higher incidence of postoperative AF in patients without beta‐blocker prophylaxis (11.7–32.4% P = 0.049).Conclusion:  Low‐dose postoperative beta‐adrenergic blockade is valuable for patients who receive these medications before off‐pump coronary artery bypass grafting procedures and may be beneficial against AF in all patients.
INDUCTION OF BONE MARROW CD34+ CELLS AND ENDOTHELIALIZATION OF POLYTETRAFLUOROETHYLENE PROSTHESES
Authors: Zhang Nan , Zhang Hongkun , Han Shiping , Feng Hua , Li Jiahui , Jin Wei , Wang Zhonggao 
Published: May 2007

Background:  The aim of the study was to investigate the endothelialization of prostheses with bone marrow CD34+ cells.Methods:  CD34+ cells were isolated from the canine bone marrow by an immune magnetic cell sorting system and induced into endothelial cells (EC) with vascular endothelial growth factor. Cells were seeded to polytetrafluoroethylene prostheses, which were implanted into the abdominal aorta artery and inferior vena cava.Results:  The isolated cells derived from bone marrow were CD34+ cells by flow cytometery, which could differentiate into EC when induced by vascular endothelial growth factor. The neointima on control prostheses was significantly thicker than that on prostheses seeded with EC at the anastomosis and the centre where the prosthesis interposed to aorta. All control prostheses (4/4) interposed to veins were occlusive and two seeded prostheses (2/8) interposed to veins were already occluded. The overall occlusion rate of prostheses interposed to veins in seeded prostheses and control prostheses were 25 and 100%, respectively. However, the neointima on seeded prostheses was significantly thicker in the vein than that in the aorta.Conclusion:  Bone marrow CD34+ cells can be induced into EC by vascular endothelial growth factor in vitro. Polytetrafluoroethylene prostheses seeded with CD34+ cells have the ideal endothelialization and patency.