bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study
Authors: Papadimitropoulos A. , Mastrogiacomo M. , Peyrin F. , Molinari E. , Komlev V.S. , Rustichelli F. , Cancedda R. 
Published: July 2007

AbstractResorbable ceramic scaffolds based on Silicon stabilized tricalcium phosphate (Si‐TCP) were seeded with bone marrow stromal cells (BMSC) and ectopically implanted for 2, 4, and 6 months in immunodeficient mice. Qualitative and quantitative evaluation of the scaffold material was performed by X‐ray synchrotron radiation computed microtomography (microCT) with a spatial resolution lower than 5 µm. Unique to these experiments was that microCT data were first collected on the scaffolds before implantation and then on the same scaffolds after they were seeded with BMSC, implanted in the mice and rescued after different times. Volume fraction, mean thickness and thickness distribution were evaluated for both new bone and scaffold phases as a function of the implantation time. New bone thickness increased from week 8 to week 16. Data for the implanted scaffolds were compared with those derived from the analysis of the same scaffolds prior to implantation and with data derived from 100% hydroxyapatite (HA) scaffold treated and analyzed in the same way. At variance with findings with the 100% HA scaffolds a significant variation in the density of the different Si‐TCP scaffold regions in the pre‐ and post‐implantation samples was observed. In particular a post‐implantation decrease in the density of the scaffolds, together with major changes in the scaffold phase composition, was noticeable in areas adjacent to newly formed bone. Histology confirmed a better integration between new bone and scaffold in the Si‐TCP composites in comparison to 100% HA composites where new bone and scaffold phases remained well distinct. Biotechnol. Bioeng. 2007; 98: 271–281. © 2007 Wiley Periodicals, Inc.
Design and application of an oscillatory compression device for cell constructs
Authors: Cassino Theresa R. , Anderson Roger , Love Brian J. , Huckle William R. , Seamans Diane K. , Forsten‐Williams Kimberly 
Published: July 2007

AbstractMechanical compression has been shown to impact cell activity; however a need for a single device to perform a broader range of parametric studies exists. We have developed an oscillatory displacement controlled device to uniaxially strain cell constructs under both static and dynamic compression and used this device to investigate gene expression in cell constructs. The device has a wide stroke (0.25–4 mm) and frequency range (0.1–3 Hz) and several loading waveforms are possible. Alginate cellular constructs with embedded equine chondrocytes were tested and viability was maintained for the 24 h test period. Off‐line mechanical testing is described and a modulus value of 18.2 ± 1.3 kPa found for alginate disks which indicates the level of stress achieved with this deformation profile. Static (15% strain) and dynamic (15% strain, 1 Hz, triangle waveform) testing of chondrocyte constructs was performed and static compression showed significantly higher collagen II expression than dynamic using quantitative RT‐PCR. In contrast, differences in matrix metalloproteinase‐3 (MMP‐3) expression were statistically insignificant. These studies indicate the utility of our device for studying cell activity in response to compression and suggest further studies regarding how the load and strain spectrum impact chondrocyte activity. Biotechnol. Bioeng. 2007; 98: 211–220. © 2007 Wiley Periodicals, Inc.
Salvage oesophagectomy after local failure of definitive chemoradiotherapy
Authors: Gardner-Thorpe J , Hardwick R H , Dwerryhouse S J 
Published: July 2007

AbstractBackgroundDefinitive chemoradiotherapy (CRT) is one treatment option for locally advanced oesophageal carcinoma. CRT typically consists of high-dose (50–66 Gy) external beam radiotherapy concurrent with 5-fluorouracil and cisplatin. When definitive CRT fails to achieve local control, salvage oesophagectomy is frequently the only treatment available that can offer a chance of long-term survival.MethodsOnline databases were searched for publications relating to salvage oesophagectomy and definitive CRT. Nine series containing a total of 105 patients were reviewed. Demographics, indications for surgery, type of resection, complications and outcome data were extracted.ResultsEach centre performed one to three salvage resections per year comprising 1·7–4·1 per cent of the oesophagectomy workload. The overall anastomotic leak rate was 17·1 per cent. The in-hospital mortality rate was 11·4 per cent. Five-year survival rates of 25–35 per cent were achieved. Prognostic factors for increased survival were R0 resection (P = 0·006) and longer interval between CRT and recurrence (P = 0·002).ConclusionSalvage resection after CRT is feasible for selected patients but is a formidable undertaking. Restaging investigations after CRT for potentially resectable tumours in fit candidates should include endoscopy and positron emission tomography–computed tomography. Salvage oesophagectomy is carried out with the goal of cure and it should be attempted only if an R0 resection is technically possible.
Genomic variants of TLR1 – It takes (TLR‐)two to tango
Authors: Schumann Ralf R. , Tapping Richard I. 
Published: July 2007

AbstractToll‐like receptors (TLR) are innate immune sensors of microbial cell wall products that initiate early host responses. The TLR2 receptor complex has been shown to contain heterodimers of TLR2 with either TLR1 or TLR6 enabling the host to detect different microbial molecules, such as lipopeptides of different chemical composition. In this issue of the European Journal of Immunology, an important role in the sensing of microbial products for I602S, a single nucleotide polymorphism (SNP) in human TLR1 has been identified. This result, in combination with another recently published report on this polymorphism elucidating a functional role in cell trafficking (surface expression of the receptor complex in individuals carrying the SNP was altered), provide genetic evidence affirming the important function of TLR1 as an essential co‐receptor for TLR2.See accompanying article: http://dx.doi.org/10.1002/eji200737034
The needs of carers of patients with anorexia and bulimia nervosa
Authors: Graap Holmer , Bleich Stefan , Herbst Florian , Trostmann Yvonne , Wancata Johannes , de Zwaan Martina 
Published: July 2007

AbstractObjectiveThis study aims to assess the degree of distress and the need for support of carers of patients with anorexia and bulimia nervosa (BN).MethodsThirty‐two carers filled out the General Health Questionnaire (GHQ‐12) and the Burden Inventory (BI). In addition, they were interviewed with a semi‐structured research interview, the Carers' Needs Assessment (CNA), to assess relevant problem areas as well as the needs for helpful interventions. Patients were interviewed with the Eating Disorder Examination (EDE) to assess the severity of the eating disorder. All patients met criteria for anorexia (n = 16) or BN (n = 16) according to DSM‐IV criteria.ResultsThe mean duration of illness was 5.6 years. The mean age of the carers was 41 years. Most of the carers were mothers or partners. In the CNA we found high numbers of problems as well as high numbers of needed interventions. The most frequently mentioned problem area was ‘disappointment caused by the chronic course of the illness, concerns about the patient's future’ and the most frequently reported need for support was ‘counselling and support by a professional’. In three problem areas carers of persons suffering from anorexia nervosa (AN) reported significantly higher scores than carers of persons suffering from BN.ConclusionsOur results suggest that carers themselves have high levels of needs which are usually not addressed in clinical practice. Copyright © 2007 John Wiley & Sons, Ltd and Eating Disorders Association.
Weight curves as predictors of short‐term outcome in anorexia nervosa inpatients
Authors: Mewes Ricarda , Tagay Sefik , Senf Wolfgang 
Published: July 2007

AbstractObjectiveThe aim of the present study was to find factors that predict the short‐term outcome of inpatients with anorexia nervosa (AN).MethodFemale inpatients with AN (N = 100) were divided into treatment completers (N = 50) and drop‐outs (N = 50). Using stepwise linear regression the relationship of demographic data, illness history and patterns of weight gain to BMI gain up to discharge were analysed.ResultsAmong drop‐outs, 69% of the variance in BMI gain up to discharge was explained by length of the first half of treatment (FHT), average weekly BMI gain and average weight loss during FHT. Among completers, 52% of the variance in BMI gain up to discharge was explained by BMI at admission and average weekly BMI gain during the second half of treatment (SHT).DiscussionWeight curves offer important information about short‐term outcome for patients with AN. Copyright © 2007 John Wiley & Sons, Ltd and Eating Disorders Association.
Downregulation of RAD17 in head and neck cancer
Authors: Zhao Ming , Begum Shahnaz , Ha Patrick K. , Westra William , Califano Joseph 
Published: July 2007

AbstractBackground.DNA repair genes play a critical role in maintaining genome stability and have been implicated in tumorigenesis. Head and neck squamous cell carcinoma (HNSCC) often shows chromosomal instability. We examined the expression of human RAD17, a DNA damage cell cycle checkpoint gene, in primary head and neck cancer tissue.Methods.Significance analysis of microarrays was applied to expression array results examining more than 12,000 genes in 7 samples of primary HNSCC and 6 samples of normal control oral epithelial tissue. Additional confirmation was performed by quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) in these samples and western blot with an additional 12 primary HNSCC and 7 normal samples, followed by loss of heterozygosity (LOH) analysis and quantitative PCR at the RAD17 locus.Results.Multiple checkpoint and DNA repair genes were downregulated in primary head and neck tumor tissue compared with normal control epithelial tissue, including hRAD17. Its Z‐score and fold change were −2.5 and 0.39, respectively. The results of normalized, quantitative RT‐PCR showed decreased expression of hRAD17 mRNA in tumor tissue (mean value 0.2166) when compared with normal tissue (mean value 0.3957, p < .05). Western blot demonstrated undetectable expression of hRAD17 protein in primary tumor tissue (0/12), while there was strong expression of hRAD17 protein in normal oral mucosal tissue (6/7). To determine possible mechanisms of inactivation, the hRAD17 locus at 5q13 was analyzed using microsatellite markers, showing 70% LOH in 30 primary HNSCCs. Quantitative PCR showed that RAD17 DNA copy number was decreased in the majority of head and neck tumor tissue samples.Conclusion.Loss of hRAD17 expression occurs frequently in HNSCC, is often due to genomic deletion, and may facilitate genomic instability in HNSCC. © 2007 Wiley Periodicals, Inc. Head Neck, 2008
Ewing sarcoma of the mandibular condyle: Multidisciplinary management optimizes outcome
Authors: Solomon Lynn W. , Frustino Jennifer L. , Loree Thom R. , Brecher Martin L. , Alberico Ronald A. , Sullivan Maureen 
Published: July 2007

AbstractBackground.Ewing sarcoma (ES) is a rare, primary malignancy of bone that occurs in childhood and early adolescence. Improved methods of diagnosis and treatment have dramatically increased survival over the last 20 years. Treatment mainstays are chemotherapy and surgical tumor resection. ES usually occurs in long bones of the axial skeleton; however, it may rarely arise in facial structures, particularly the mandible. In these cases, resection presents a challenging postsurgical reconstruction.Methods and Results.We present the clinical findings and management of a case of ES that developed in the left mandibular condyle of a 15‐year‐old female. Chemotherapy and segmental mandibulectomy were used to achieve local control. An innovative temporomandibular joint reconstruction was successfully accomplished using a microvascular fibular free flap and conchal cartilage graft.Conclusion.Multidisciplinary management in diagnosis, treatment, and restoration of function produced an optimal result that eliminated disease and preserved aesthetics and quality of life. © 2007 Wiley Periodicals, Inc. Head Neck 2008
Concurrent platinum‐based chemotherapy and simultaneous modulated accelerated radiation therapy for locally advanced squamous cell carcinoma of the tongue base
Authors: Lawson Joshua D. , Otto Kristen , Chen Amy , Shin Dong M. , Davis Lawrence , Johnstone Peter A. S. 
Published: July 2007

AbstractBackground.Randomized data support use of chemotherapy concurrently with radiation in treatment of advanced squamous cell carcinoma (SCC) of the oropharynx. Intensity modulated radiation therapy (IMRT) is increasingly being used to deliver such radiotherapy; no published reports specifically describe results of chemotherapy with IMRT for SCC of the base of tongue (BOT). We present outcomes data using simultaneous modulated accelerated radiation therapy (SMART) combined with platinum‐based chemotherapy in treatment of locally advanced SCC of the BOTMethods.The records of the Otolaryngology/Head and Neck Surgery Department of Emory University were screened for patients undergoing definitive chemoradiotherapy for SCC of the BOT. Radiation Oncology records were reviewed for dosimetry and prescription data. Hospital and clinic records were reviewed for control and toxicity data. All patients were treated definitively with platinum‐based chemotherapy and once‐daily RT. Median dose and dose per fraction to sites of gross primary or nodal disease, clinically involved neck, and clinically uninvolved neck were 70.29 Gy (2.13 Gy/fx), 63.03 Gy (1.91 Gy/fx), and 57.75 Gy (1.75 Gy/fx), respectivelyResults.Between January 2003 and August 2005, 34 patients underwent definitive therapy for SCC of the BOT using SMART and chemotherapy. Follow‐up was documented in all cases (median, 20.1 months). There have been 3 distant failures and 3 locoregional failuresConclusion.With moderate follow‐up, chemotherapy and SMART contributes to excellent results, with 24‐month actuarial overall survival and local control of 90% and 92%, respectively. Toxicity may be increased, however, with 15% of patients developing esophageal stricture or stenosis. © 2007 Wiley Periodicals, Inc. Head Neck 2008
Fas single nucleotide polymorphisms and risk of thyroid and salivary gland carcinomas: A case‐control analysis
Authors: Ho Tang , Li Guojun , Zhao Chong , Zheng Rong , Wei Qingyi , Sturgis Erich M. 
Published: July 2007

AbstractBackground.The purpose of this study was to examine the association between 4 Fas single nucleotide polymorphisms (SNPs) and risk of differentiated thyroid carcinoma (DTC) and salivary gland carcinoma (SGC).Methods.We conducted a case‐control study including 279 DTC cases, 165 benign thyroid disease (BTD) cases, 154 SGC cases, 61 benign salivary gland disease (BSGD) cases, and 510 controls.Results.The A744G SNP genotype distribution was significantly different between subjects with SGC or BSGD and controls, while that of the A18272G SNP was significantly different between subjects with DTC or SGC and controls. Risk of SGC was significantly elevated for the 22628 heterozygous CT genotype (odds ratio [OR] = 1.5, p = .050), and risk of BSGD was elevated for the 22628 homozygous TT genotype (OR = 2.9, p = .023).Conclusion.Fas C22628T SNP may be associated with risk of SGC and BSGD, but none of the investigated Fas SNPs was associated with risk of DTC. © 2007 Wiley Periodicals, Inc. Head Neck 2008
Spontaneous regression of cutaneous head and neck melanoma: Implications for the immunologic control of neoplasia
Authors: Dunn Gavin P. , Lewis James S. , Sunwoo John B. , Uppaluri Ravindra 
Published: July 2007

AbstractBackground.Spontaneous regression of cancer in the head and neck is a rare event. Moreover, there are rare reported cases of spontaneous regression of primary head and neck melanoma with accompanying immunohistochemical analysis of the tumor.Methods.We used detailed preoperative and postoperative pathologic examination of a lesion in the right supraclavicular region.Results.Pathologic examination of the initial specimen identified a melanoma of superficial spreading type with vertical growth and a thickness of 1.8 mm. The excised specimen demonstrated a complete regression of the melanoma with a florid host inflammatory response predominantly composed of a histiocytic reaction.Conclusion.The case presented illustrates histopathologic findings occurring in a head and neck melanoma as it is undergoing spontaneous regression. These findings point to a potentially critical role for histiocytes in effecting tumor elimination. Pathologic analysis of spontaneous head and neck melanoma regression will ultimately facilitate an improved understanding of naturally‐occurring tumor elimination. © 2007 Wiley Periodicals, Inc. Head Neck 2008
Overexpression and role of the ATPase and putative DNA helicase RuvB-like 2 in human hepatocellular carcinoma
Authors: Rousseau Benoît , Ménard Ludovic , Haurie Valérie , Taras Danièle , Blanc Jean-Frédéric , Moreau-Gaudry François , Metzler Philippe , Hugues Michel , Boyault Sandrine , Lemière Sylvie , Canron Xavier , Costet Pierre , Cole Michael , Balabaud Charles , Bioulac-Sage Paulette , Zucman-Rossi Jessica , Rosenbaum Jean 
Published: July 2007

Abstract

Using a proteomic analysis of human hepatocellular carcinoma (HCC), we identified the overexpression in 4 tumors of RuvB-like 2 (RUVBL2), an ATPase and putative DNA helicase known to interact with β-catenin and cellular v-myc myelocytomatosis viral oncogene homolog (c-myc). RUVBL2 expression was further analyzed in tumors with quantitative reverse-transcription polymerase chain reaction analysis and immunohistochemistry; in addition, RUVBL2 expression in a HuH7 cell line was silenced by small interfering RNA or increased with a lentiviral vector. RUVBL2 messenger RNA overexpression was confirmed in 72 of 96 HCC cases, and it was associated with poorly differentiated tumors (

P

= 0.02) and a poor prognosis (

P

= 0.02) but not with β-catenin mutations or c-myc levels. Although RUVBL2 was strictly nuclear in normal hepatocytes, tumoral hepatocytes exhibited additional cytoplasmic staining. There was no mutation in the coding sequence of RUVBL2 in 10 sequenced cases. Silencing RUVBL2 in HuH7 HCC cells reduced cell growth (

P

< 0.001) and increased apoptosis, as shown by DNA fragmentation (

P

< 0.001) and caspase 3 activity (

P

< 0.005). This was associated with an increased expression of several proapoptotic genes and with an increased conformational activation of Bak-1 and Bax. On the other hand, HuH7 cells with an overexpression of RUVBL2 grew better in soft agar (

P

< 0.03), had increased resistance to C2 ceramide–induced apoptosis (

P

< 0.001), and gave rise to significantly larger tumors when injected into immunodeficient Rag2/γc mice (

P

= 0.016).

Conclusion:

RUVBL2 is overexpressed in a large majority of HCCs. RUVBL2 overexpression enhances tumorigenicity, and RUVBL2 is required for tumor cell viability. These results argue for a major role of RUVBL2 in liver carcinogenesis. (HEPATOLOGY 2007.)
Differential priming of CD8 and CD4 T-cells in animal models of autoimmune hepatitis and cholangitis
Authors: Derkow Katja , Loddenkemper Christoph , Mintern Justine , Kruse Nils , Klugewitz Katja , Berg Thomas , Wiedenmann Bertram , Ploegh Hidde L. , Schott Eckart 
Published: July 2007

Abstract

The pathogenesis of autoimmune liver diseases is poorly understood. Animal models are necessary to investigate antigen presentation and priming of T-cells in the context of autoimmunity in the liver. Transgenic mouse models were generated in which the model antigen ovalbumin is expressed in hepatocytes (TF-OVA) or cholangiocytes (ASBT-OVA). Transgenic OT-I (CD8) or OT-II (CD4) T-cells specific for ovalbumin were adoptively transferred into TF-OVA and ASBT-OVA mice to induce

in vivo

priming of antigen-specific T-cells. T-cell migration and activation, as well as induction of liver inflammation, were studied. OT-I T-cells preferentially located to the liver of both mouse strains whereas no migration of OT-II T-cells to the liver was observed. OT-I T-cells proliferated in the liver of TF-OVA mice and the liver and liver draining lymph nodes of ASBT-OVA mice. OT-II CD4 T-cells were activated in spleen and liver draining lymph node of TF-OVA mice but not in ASBT-OVA mice. Transfer of OT-I T-cells led to histologically distinct inflammatory conditions in the liver of ASBT-OVA and TF-OVA mice and caused liver injury as determined by the elevation of serum alanine aminotransferase.

Conclusion:

An antigen expressed in hepatocytes is presented to CD8 and CD4 T-cells, whereas the same antigen expressed in cholangiocytes is presented to CD8 but not CD4 T-cells. In both models, activation of CD8 T-cells occurs within the liver and causes liver inflammation. The models presented here are valuable to investigate the priming of T-cells in the liver and their role in the development of autoimmune disease of the liver. (HEPATOLOGY 2007.)
Association of the SUV39H1 histone methyltransferase with the DNA methyltransferase 1 at mRNA expression level in primary colorectal cancer
Authors: Kang Mi Yeon , Lee Bo Bin , Kim Young‐Ho , Chang Dong Kyoung , Kyu Park Seo , Chun Ho‐Kyung , Song Sang Yong , Park Joobae , Kim Duk‐Hwan 
Published: July 2007

AbstractThis study was aimed at investigating the involvement of the SUV39H1 histone methyltransferase on the epigenetic change of euchromatic promoter in colorectal cancer. We retrospectively analyzed the mRNA levels of SUV39H1 and the promoter methylation of the p14ARF, p16INK4a and HLTF genes as well as the mRNA levels of DNA methyltransferase 1 (DNMT1) in fresh frozen tissues from 219 colorectal cancer patients. The mRNA levels of the SUV39H1 and DNMT1 were assessed via quantitative real‐time PCR and the methylation profiles of the CpG islands were determined using methylation‐specific PCR. The mRNA levels of SUV39H1 and DNMT1 were elevated in 25% and 42% of 219 colorectal cancers, respectively. The hypermethylation of the p14ARF, p16INK4a and HLTF genes occurred in 36%, 51% and 34% of the patients. The elevated mRNA levels of SUV39H1 were not associated with the hypermethylation of the 3 genes. However, the mRNA levels of DNMT1 were significantly different between patients with elevated mRNA levels of SUV39H1 and those without (1.62 ± 0.84, 0.91 ± 0.81, respectively; p = 0.007). Patients with elevated mRNA levels of SUV39H1 showed a higher prevalence of DNMT1 elevation than those without (61 vs. 35%, p = 0.0008). Patients with an elevated mRNA level of SUV39H1 had a 2.71 (95% CI = 1.09–4.48, p = 0.002) times greater risk of an elevated mRNA level of DNMT1, after controlling for age and gender. In conclusion, the present study suggests that SUV39H1 is significantly associated with DNMT1, but not with euchromatic promoter methylation in colorectal cancer. © 2007 Wiley‐Liss, Inc.