bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epimedin A inhibits the PI3K/AKT/NF-κB signalling axis and osteoclast differentiation by negatively regulating TRAF6 expression
Authors: li jun , Wei Jia J , Wu Cen H , Zou Tao , Zhao Hong , Huo Tianqun , Wei Chengjian , Yang Ting 
Published: March 2024

Abstract

Objective: Epimedin A (EA) has been indicated to suppress the extensive osteoclastogenesis and bone resorption, of which the effects remain incompletely understood. The aim of our study was to investigate the effects of EA on osteoclastogenesis and bone resorption, so as to explore its corresponding signal pathway.
Methods: Rats were randomly received sham-operation and ovariectomy, with alendronate as the positive group. The therapeutic effect of EA on osteoporosis was systematically analyzed by measuring bone mineral density and bone biomechanical properties. In vitro, RAW264.7 cells were treated with receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) to induce osteoclast differentiation. Cell viability assay, tartrate-resistent acid phosphatase (TRAP) staining, and immunofluorescence were used to elucidate the influences of EA on osteoclastogenesis. In addition, the expression of bone differentiation related proteins or genes were evaluated using western blot analysis or quantitative polymerase chain reaction (PCR), respectively.
Results: After 3 months of oral EA intervention, OVX rats showed increased bone density, relative bone volume, trabecular thickness, and trabecular number, along with reduced trabecular separation. EA dose-dependently improved bone density and trabecular microarchitecture in de-ovulated rats. Additionally, EA inhibited expressions of TRAP and NFATc1 in OVX rats. In addition, the in vitro results indicated that EA may inhibit osteoclast differentiation by suppressing the TRAF6/PI3K/AKT/NF-κB pathway. Further studies revealed that osteoclast differentiation, which was originally inhibited by EA, was reversed when the TRAF6 gene was overexpressed.
Conclusion: The findings indicated that EA may hold promise as a potential therapeutic agent for the treatment of osteoporosis.
Stripping Voltammetry and Chemometric Modelling Scheme for Robust, Sensitive, and Selective Electrochemical Sensing of As3+ ions in Water
Authors: Bansod Babankumar S , Antil Monika 
Published: March 2024

Abstract
The ionic form of Arsenic As3+ is the most toxic, causing severe health problems like cancer, cardiovascular diseases, and developmental disorders. Therefore, researchers have contributed significantly to its removal and detection work. However, there is still a need for accurate, trustworthy, and sensitive methods that may be employed at field sites for detecting Arsenic ions at nano levels. In the present study, we report a novel and robust electrochemical sensing platform for the selective and sensitive detection of As3+ ions in aqueous samples. The method combines the high sensitivity of stripping voltammetry with the data analytics and power of Chemometric modelling to achieve superior performance. Optimising key instrumental parameters such as deposition potential, deposition time, and scan rate improves the analytical response. To address potential interferences from co-existing ions, a Chemometric modelling approach based on principal component analysis (PCA) is employed to ensure the selection of predictor variables. This multivariate technique effectively discriminates As(III) signals from background noise and interfering species, enhancing selectivity and accurate quantification. The proposed method demonstrates a threshold limit and sensitivity of 0.4nM and 176.34µA/µM, for Arsenic, which is less than the World Health Organization (WHO) permissible limit in drinking water. Furthermore, the method shows good repeatability and stability, making it suitable for real-world water analysis applications. This novel sensing platform offers a promising alternative for sensitive, selective, and robust As(III) detection in water samples, contributing to improved water quality monitoring and public health protection.
Clinical profile Of patients with Urinary Diversion (Mainz II) For Women With Irreparable Vesico-Vaginal Fistula (VVF) In Eritrea, 2022: A Retrospective Study
Authors: daniel nahom 
Published: March 2024

Abstract
Introduction: Mainz II urinary diversion procedures are increasingly being performed in the developing world for irreparable obstetric fistulas. Our goal was to review the long-term complications of continent urinary diversion and to evaluate the feasibility of the recommended follow-up protocols in a country with limited resources.
Methods: The medical records of patients who underwent urinary diversion or who are under follow up for irreparable vesico-vaginal fistula (VVF) at National Fistula center (NFC) in Mendefera, Eritrea, between February 2004 and December 2021, were reviewed. Descriptive statistics was used to depict complications and surgical outcomes by using frequencies and percentages.
Results: Urinary diversions were performed on 47 patients. The average duration of stay after surgery was 18 days. Metabolic panel and Arterial Blood Gases (ABG) were found only in 26 of the records which showed hyperchloremic acidosis in 22 (84.7%) and decreased kidney function in 8 (30.7%) of the women. Night time incontinence was found in 24 (51.1%) of the cases with 9 (19.1%) experiencing symptomatic renal infections which required admission. Three of the patients developed pouch stones and two patients developed hydronephrosis. Five patients got pregnant after diversion. Eleven (23.4 %) women were lost to follow up and 10 (21.3 %) died with majority (70%) of them with unknown cause of death.
Conclusion: On top of the long-term complications following diversion, there was substantial loss to follow-up and death after urinary diversion. Therefore, innovative approaches and continuous financial support to ensure patient return or local follow-up in home regions must be developed to overcome barriers to guarantee continuity of care and to decrease the morbidity and mortality of these women.
Exploring the association between adoptive parents’ attachment orientations and adoptees’ emotional and behavioral difficulties: The role of parents’ self-compassion and mindful parenting
Authors: Rato Matilde Paulo , Canavarro Maria Cristina , Pires Raquel 
Published: March 2024

Abstract
This study aimed to explore the association between adoptive parents’ attachment orientations and adoptees’ emotional and behavioral difficulties while examining the mediating role of adoptive parents’ self-compassion and mindful parenting in this relationship, and to explore the extent to which the above-mentioned associations differ from those observed in non-adoptive families. Using convenience sampling and a cross-sectional study design, a sample of adoptive parents (n = 400) and non-adoptive parents (n = 333) of children aged between 2 and 17 years old was collected through an online self-report survey. Serial mediation models showed that adoptive parents’ attachment-related avoidance and attachment-related anxiety were associated with adoptees’ emotional and behavioral difficulties; these relationships were found to be serially mediated by self-compassion and mindful parenting. Mediation analyses also showed that adoptive parents’ attachment-related anxiety was associated with adoptees’ emotional and behavioral difficulties through mindful parenting alone. Moderated mediation analyses suggested that the association between parents’ attachment anxiety and children’s emotional and behavioral difficulties through self-compassion and mindful parenting was significantly moderated by the type of family. These findings contribute to a better understanding of the role adoptive parents might have on adoptees’ postplacement emotional and behavioral difficulties, highlighting the importance of their levels of self-compassion and mindful parenting; they add new information for planning post-adoption therapeutic services aimed to foster a positive adoptive parent-child relationship and, thus, the wellbeing of families challenged by adoption circumstances.
Associations of blood and urinary heavy metals with stress urinary incontinence risk among adults in NHANES, 2003–2018
Authors: Fu Maoling , Zhu Zifan , Xiang Yechen , Yuan Quan , Yang Qiaoyue , Yu Yaqi , Song Wenshuai , Li Xinyu , Yu Genzhen 
Published: March 2024

Abstract

Background: People come into contact with heavy metals in various ways in their daily lives. Accumulating evidence shows that toxic metal exposure is hazardous to human health. However, limited information is available regarding the impact of metal mixtures on stress urinary incontinence (SUI).
Methods: In this specific survey, data from the National Health and Nutrition Examination Survey (NHANES) for eight cycles from 2003 to 2018. Logistic regression, weighted quantile sum (WQS) regression, quantile-based g-computation (qgcomp), Bayesian kernel machine regression (BKMR) analyses, and Restricted cubic spline (RCS) analysis were used to explore the independent and comprehensive associations of heavy metal exposure with SUI risk.
Results: A total of 10622 U.S. adults were included, and 2455 (23.11%) were diagnosed with SUI. In the single-exposure model, blood cadmium (Cd), lead (Pb), mercury (Hg), urinary Cd, Pb, and cesium (Cs) were found to be positively connected with SUI risk. Moreover, WQS, qgcomp, and BKMR models consistently demonstrated blood and urinary metals-mixed exposure were positively associated with the risk of SUI, and emphasized that blood Pb and Cd and urinary Cd and Cs were the main positive drivers, respectively. This association was more pronounced in the young and middle-aged group (20-59 years old) and the female group.
Conclusions: Using large-scale national data, we found that even after adjusting for twelve confounding factors, heavy metal exposure significantly increases the risk of SUI in the general population.
The Relationship Between Adolescents' Physical Activity and Circadian Rhythms on Sleep
Authors: Liu Daoli , Li Hengfen 
Published: March 2024

Abstract
Objective: To explore the correlation between physical activity, circadian rhythms, and sleep in adolescents, and analyze the influencing factors of sleep quality in this population.
Methods: A total of 381 high school students were selected through cluster sampling in a specific high school. The Pittsburgh Sleep Quality Index (PSQI) was employed to categorize the participants into a good sleep quality group (n=199) and a poor sleep quality group (n=182). Comprehensive assessments were conducted using the Hamilton Depression Rating Scale (HAMD), Hamilton Anxiety Rating Scale (HAMA), PSQI, Morningness-Eveningness Questionnaire-5 (MEQ-5), and International Physical Activity Questionnaire Short Form (IPAQ-SF).
Results: The prevalence of poor sleep quality is 47.8%. The BMI in the poor sleep quality group is higher than that in the good sleep quality group, and the male ratio is lower than that in the good sleep quality group. The poor sleep quality group exhibits significantly higher levels of depression, anxiety, evening chronotype, and low physical activity compared to the good sleep quality group.Spearman rank correlation analysis revealed a positive correlation between the PSQI total score and HAMA and HAMD scores, and a negative correlation with physical activity and MEQ-5 scores. Binary logistic stepwise regression analysis identified lack of physical activity, eveningness chronotype, anxiety, and depression as risk factors for poor sleep quality.
Conclusion: Adolescent sleep quality is correlated with gender, BMI, anxiety, depression, chronotype, and physical activity levels. The findings highlight the importance of considering these factors in interventions aimed at improving sleep health in adolescents.
Retention of Implant Retained Obturator Using Two Implant Placement Configurations for Maxillectomy Cases: In-Vitro Study
Authors: Aboseada Nourhan. I. , Mohamed Faten. S. , El-shabrawy Sonia. M. 
Published: March 2024

Abstract

Background
Implant-retained obturators for maxillectomy cases have several advantages over traditional obturators for them. Appropriate prosthetic design for specific condition after maxillary resection is essential for improvement of retention, stability of implant retained obturator. Implant placement configuration for maxillary obturators had several challenges.
Objectives
Retention evaluation of obturators retained by three implants placed with two different placement configurations.
Material and methods
Three implants arranged in linear configuration and other arranged in nonlinear configuration were inserted into two identical epoxy resin maxillary models of completely edentulous unilateral maxillary defect (Brown’s class IIb). Two equal sets of twenty-six obturators were constructed; each with different implant placement configuration. Group I include thirteen obturators retained by three implants with linear configuration while Group II include thirteen obturators retained by three implants with nonlinear configuration. Both groups included the same attachment design (non-splinted Ball attachment). Using a cyclic loading machine that served as a dental mastication simulator, each obturator was subjected to 500 tension-compression cycles. Using the universal testing machine, each obturator was removed at a speed of 50 mm/min for the crosshead. To evaluate the initial retention and loss of retention between the two groups, peak load to dislodgement was measured both before and after the cyclic test.
Results
Nonlinear implant placement configuration for class IIb maxillectomy showed more initial and final retentive force than linear implant placement configuration Also, after cyclic tension-compression test of 500 cycles, all implant-retained obturators lost significant portions of its initial retention but group I (linear configuration) lost a higher percentage of its retention compared to group II (nonlinear configuration).
Conclusions
non-linear implant placement is more retentive than linear and lost less retention after usage.
Enhanced Tiny Face Detection and Perspective Transformation for Attendance Tracking in Enclosed Venues
Authors: Abdulaziz F. , Mangoud M. A. , Mattar E. A. 
Published: March 2024

Abstract
Tiny face detection in enclosed spaces like auditoriums or parliament chambers presents a unique challenge in computer vision. Traditional face detection methods often struggle to accurately identify and track individuals, especially when faces are small or partially obscured. Addressing this issue is crucial for applications such as tracking the attendance of parliamentary members within indoor chambers. This paper presents an innovative approach for tracking the attendance of members of parliament using computer vision techniques. The applied approach leverages an improved version of YOLOv7 face detection method, enhanced with the Contextual Block Attention Module (CBAM) and head extension to optimize feature learning for tiny face detection. CBAM selectively focuses on critical features, while the additional head captures small face details. The model outperforms other object detection methods, showing substantial improvements in recall (26.76%) and small but notable gains in precision, F1-score, and mAP@0.5 (1.81%, 8.45%, and 3.03%, respectively). This approach holds promise for object detection tasks, though its performance may vary across different datasets and scenarios. Also, the paper presents a perspective transformation of members locations into bird's eye views for the venue, employing real-time face detection with a single camera to monitor and analyze attendance.
Factors associated with mental health literacy and demand for mental health services among older adults in China: a cross-sectional study
Authors: Chen Haihui , Ma Zijuan , Huang Ruidian , Liu Yujing , Li Lianghua , Zhou Zhengyuan , Wang Dongfang , Wang Wei , Lin Junxu , Lin Weishi , Yin Luning , Zhang Rui , Mo Xuan , Fan Yunge , Fan Fang 
Published: March 2024

Abstract
Background
There is limited research on the level of mental health literacy (MHL) among older adults and their demand and willingness for mental health services. This study investigated the factors associated with them among Chinese older adults. Additionally, we further compared these relationships between older adults living alone and those not living alone.
Methods
This study is a cross-sectional study conducted among 494 older adults in Guangzhou, China. Sociodemographic characteristics, frequency of participating in community-based elderly activities, mental health status, MHL, and demand and willingness for mental health services were assessed through self-reported questions. A series of logistic regression analyses were conducted to examine factors associated with the MHL and demand and willingness for mental health services.
Results
The awareness rates for the three MHL-related questions were 73.7%, 65.3%, and 73.5% respectively, with 62.0% and 69.6% of the participants indicating the demand and willingness, respectively, for mental health services. Frequently participating in elderly activities increased the likelihood of awareness of the MHL-related questions (ORs = 2.92–4.18, CIs = [1.50,9.28]), as well as the demand and willingness for mental health services (ORs = 2.16–2.24, CIs = [1.19,4.20]). Similar significant associations were found among the older adults who were not living alone but not among those living alone.
Conclusions
To enhance older adults' MHL and increase their willingness for mental health services, given the results of this study, policymakers are advised to develop targeted community-based activities and to focus in particular on living-alone groups.
Ephestia kuehniella eggs as a surrogate host to test feeding preference and intraguild interactions between and Macrolophus pygmaeus and Trichogramma achaeae
Authors: Soares António Onofre , Durão Ana C. , Oliveira Maria Luísa , Arruda Patrícia , Borges Isabel 
Published: March 2024

Abstract
Mass rearing of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) eggs is a laborious task requiring investment of time, equipment and man hour work. The use of a surrogate prey/host, as Ephestia kuehniella Zeller (Lepidoptera: Pyralidae), easily obtained and maintain, could be an option to undertake experiments. The aim of this study was to test to what extent the eggs of E. kuehniella is a suitable surrogate host of T. absoluta eggs to test for feeding preference and intraguild interactions between Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) and Trichogramma achaeae Nagaraja and Nagarkatti (Hymenoptera: Trichogrammatidae). Our results shows that E. kuehniella eggs is a suitable surrogate host to test for feeding preference and intraguild interactions between natural enemies, taking in consideration that i) under single diet, feeding on T. absoluta eggs is expected to be twice that on E. kuehniella, ii) whether offering T. absoluta or E. kuehniella under different ratios of parasitized vs unparasitized eggs, feeding preference was always toward non-parasitized eggs iii) conspecific experiments with increasing density of M. pygmaeus females, the results revel an overall decrease on feeding consumption of 38,7% of E. kuehniella compared with T. absoluta eggs but experiments with T. achaeae show an overall increase in parasitism rate of 78,2% of eggs toward E. kuehniella eggs iv) Heterospecific experiments with increasing density of both natural enemies, when using E. kuehniellaeggs we found an overall decrease on feeding consumption of 30% and an increase of parasitism rate of 65%.
Unmet needs and harm reduction preferences of syringe services program participants: Differences by co-use of street opioids and methamphetamine
Authors: Sun Rachel , Sauda Tonazzina , Hoopsick Rachel A. 
Published: March 2024

Abstract
Background
The current fourth wave of the United States opioid overdose epidemic is characterized by the co-use of opioids and stimulants, including street opioids and methamphetamine. The co-use of these two drugs, known as “goofballing,” is associated with higher risk for several adverse outcomes, including more frequent injections, greater health risks, and higher morbidity. Considering these differences, this unique subpopulation of people who inject drugs (PWID) may also have unique unmet needs and/or harm reduction preferences.
Methods
We collected self-reported data from participants (N = 50) of a syringe services program (SSP), including basic needs and harm reduction preferences. Using bivariate analyses, we examined for differences between SSP participants who do and do not co-use street opioids and methamphetamine.
Results
In the overall sample, the mean level of need was highest for bus passes or other transportation, a person who can help you get the services you need, medication for opioid use disorder, and a job or job training. Additionally, all participants reported being either interested or very interested in fentanyl test strips, safe consumption sites, delivery of syringe service supplies, and delivery of naloxone. Those who endorsed co-use had a greater need for food, healthcare, substance use disorder treatment, a support person to help them access needed services, and bus passes/transportation.
Conclusions
Unmet needs were prevalent, and the desire for more harm reduction services was high among these PWID. Results also suggest people who co-use street opioids and methamphetamine may have the greatest unmet needs and desire for additional harm reduction services.
Effect of carbon dioxide (CO 2 ) deprivation on Fibroblast cells viability and morphology
Authors: Greco I. , Machrafi H. , Iorio C. S. 
Published: March 2024

Abstract
In applications related to remote areas and microgravity experiments, access to proper CO2 incubation for in vitro experiments is difficult and, in some cases, impossible. It is important to assess the time period in which one can handle and transport cells whilst maintaining a reasonable viability. The effects of carbon dioxide (CO2) deficiency, in an in vitro environment, on the viability and morphology of fibroblast cells are looked at in this work. A CO2-free environment was provided to fibroblast cells, and their behavior was tracked over an established amount of time. The measured viability of the cells was higher than 80% up until 7h, where it appeared that the cell shape did not change much as was visualized by the microscope and quantified by measuring the average width and length of the cells. The cell shape started to change considerably at 10 h accompanied by a stronger viability decrease, i.e. 9%/h vs 3%/h. After 16 hours, the experiment came to an end with almost total cell viability loss.