bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mid-term results of a prospective study for aortic dissection with a gutter-plugging chimney stent graft
Authors: Shu Chang , Wan Zicheng , Luo Mingyao , Fang Kun , Hu Jia , Zuo Jian , Li Xiaoqiang , Li Quanming , He Hao , Li Xin 
Published: April 2024

Abstract

OBJECTIVES
Our goal was to access early and mid-term outcomes of a gutter-plugging chimney stent graft for treatment of Stanford type B aortic dissections in the clinical trial Prospective Study for Aortic Arch Therapy with stENt-graft for Chimney technology (PATENCY).

METHODS
Between October 2018 and March 2022, patients with Stanford type B aortic dissections were treated with the Longuette chimney stent graft in 26 vascular centres. The efficiency and the incidence of adverse events over 12 months were investigated.

RESULTS
A total of 150 patients were included. The technical success rate was 99.33% (149/150). The incidence of immediate postoperative endoleak was 5.33% (8/150, type I, n = 6; type II, n = 1; type IV, n = 1) neurologic complications (stroke or spinal cord ischaemia); the 30-day mortality was 0.67% (1/150) and 1.33% (2/150), respectively. During the follow-up period, the median follow-up time was 11.67 (5–16) months. The patent rate of the Longuette graft was 97.87%. Two patients with type I endoleak underwent reintervention. The follow-up rate of the incidence of retrograde A type aortic dissection was 0.67% (1/150). There was no paraplegia, left arm ischaemia or stent migration.

CONCLUSIONS
For revascularization of the left subclavian artery, the Longuette chimney stent graft can provide an easily manipulated, safe and effective endovascular treatment. It should be considered a more efficient technique to prevent type Ia endoleak. Longer follow-up and a larger cohort are needed to validate these results.

CLINICAL TRIAL REGISTRY NUMBER
NCT03767777
Same-day discharge after atrial fibrillation ablation under a nurse-coordinated standardized protocol
Authors: Espinosa Teresa , Farrus Anna , Venturas Montserrat , Cano Alba , Vazquez-Calvo Sara , Pujol-Lopez Margarida , Eulogio-Valenzuela Frida , Guichard Jean-Baptiste , Falzone Pasquale V , Graterol Freddy R , Freixa Xavier , Tolosana Jose M , Guasch Eduard , Porta-Sanchez Andreu , Arbelo Elena , Brugada Josep , Sitges Marta , Mont Lluis , Roca-Luque Ivo , Althoff Till F 
Published: April 2024

Abstract

Aims
Same-day discharge (SDD) after atrial fibrillation (AF) ablation is an effective means to spare healthcare resources. However, safety remains a concern, and besides structural adaptations, SDD requires more efficient logistics and coordination. Therefore, in this study, we implement a streamlined, nurse-coordinated SDD programme following a standardized protocol.

Methods and results
As a dedicated SDD coordinator, a nurse specialized in ambulatory cardiac interventions was in charge of the full SDD protocol, including eligibility, patient flow, in-hospital logistics, patient education, and discharge as well as early post-discharge follow-up by smartphone-based virtual visits. Patients planned for AF ablation were considered eligible if they had a left ventricular ejection fraction (LVEF) ≥35%, with basic support at home and accessibility of the hospital within 60 min also forming a part of the eligibility criteria. A total of 420 consecutive patients were screened by the SDD coordinator, of whom 331 were eligible for SDD. The reasons for exclusion were living remotely (29, 6.9%), lack of support at home (19, 4.5%), or LVEF &lt;35% (17, 4.0%). Of the eligible patients, 300 (91%) were successfully discharged the same day. There were no major post-SDD complications. Rates of unplanned medical attention (19, 6.3%) and 30-day readmission (5, 1.6%) were extremely low and driven by femoral access–site complications. These were significantly reduced upon the introduction of compulsory ultrasound-guided punctures after the initial 150 SDD patients (P = 0.0145). Standardized SDD coordination resulted in efficient workflows and reduced the total workload of the medical staff.

Conclusion
Same-day discharge after AF ablation following a nurse-coordinated standardized protocol is safe and efficient. The concept of ambulatory cardiac intervention nurses functioning as dedicated coordinators may be key in the future transition of hospitals to SDD. Ultrasound-guided femoral puncture virtually eliminated relevant femoral access–site complications in our cohort and should therefore be a prerequisite for SDD.
Evolutionary divergence of plasticity in brain morphology between ecologically divergent habitats of Trinidadian guppies
Authors: Axelrod Caleb J , Yang Yusan , Grant Eleanor , Fleming Jorie , Stone Isabel , Carlson Bruce A , Gordon Swanne P 
Published: April 2024

Abstract
Phenotypic plasticity is critical for organismal performance and can evolve in response to natural selection. Brain morphology is often developmentally plastic, affecting animal performance in a variety of contexts. However, the degree to which the plasticity of brain morphology evolves has rarely been explored. Here, we use Trinidadian guppies (Poecilia reticulata), which are known for their repeated adaptation to high-predation (HP) and low-predation (LP) environments, to examine the evolution and plasticity of brain morphology. We exposed second-generation offspring of individuals from HP and LP sites to 2 different treatments: predation cues and conspecific social environment. Results show that LP guppies had greater plasticity in brain morphology compared to their ancestral HP population, suggesting that plasticity can evolve in response to environmentally divergent habitats. We also show sexual dimorphism in the plasticity of brain morphology, highlighting the importance of considering sex-specific variation in adaptive diversification. Overall, these results may suggest the evolution of brain morphology plasticity as an important mechanism that allows for ecological diversification and adaptation to divergent habitats.
Indirect costs of reproduction and the tradeoff between offspring size and number: a framework illustrated by fitness costs and benefits of ovarian fluid
Authors: Johnson Darren W , Chhor James T , Shelley Callyn E , Siegfried Emma J 
Published: April 2024

Abstract
The theory describing the evolution of offspring size often assumes that the production cost per unit volume is the same for small and large offspring. However, this may not be true if indirect costs of reproduction (e.g., material and energetic costs of supporting offspring development) scale disproportionately with offspring size. Here we show how direct and indirect costs of reproduction can be explicitly modeled within the Smith–Fretwell framework and how observations of size-number relationships can thus be used to evaluate indirect costs. We applied this analysis to measures of egg volume and fecundity for over 300 individuals of a coastal fish species and found that the tradeoff was much stronger than the expected inverse (fecundity scaled with volume−1.843). Larger offspring were thus more expensive to produce. For our study species, an important indirect cost was that larger eggs were accompanied by disproportionately more ovarian fluid. Calorimetry and removal experiments were used to further measure both the energetic costs and fitness benefits of ovarian fluid. In addition, we show that indirect costs of reproduction can intensify size-number tradeoffs in a variety of fishes. Indirect costs of reproduction can be large and may therefore play an important role in the evolution of offspring size.
Proteomic insight into arabinogalactan utilization by particle-associated Maribacter sp. MAR_2009_72
Authors: Kalenborn Saskia , Zühlke Daniela , Riedel Katharina , Amann Rudolf I , Harder Jens 
Published: April 2024

Abstract
Arabinose and galactose are major, rapidly metabolized components of marine particulate and dissolved organic matter. In this study, we observed for the first time large microbiomes for the degradation of arabinogalactan and report a detailed investigation of arabinogalactan utilization by the flavobacterium Maribacter sp. MAR_2009_72. Cellular extracts hydrolysed arabinogalactan in vitro. Comparative proteomic analyses of cells grown on arabinogalactan, arabinose, galactose, and glucose revealed the expression of specific proteins in the presence of arabinogalactan, mainly glycoside hydrolases (GH). Extracellular glycan hydrolysis involved five alpha-l-arabinofuranosidases affiliating with glycoside hydrolase families 43 and 51, four unsaturated rhamnogalacturonylhydrolases (GH105) and a protein with a glycoside hydrolase family-like domain. We detected expression of three induced TonB-dependent SusC/D transporter systems, one SusC, and nine glycoside hydrolases with a predicted periplasmatic location. These are affiliated with the families GH3, GH10, GH29, GH31, GH67, GH78, and GH115. The genes are located outside of and within canonical polysaccharide utilization loci classified as specific for arabinogalactan, for galactose-containing glycans, and for arabinose-containing glycans. The breadth of enzymatic functions expressed in Maribacter sp. MAR_2009_72 as response to arabinogalactan from the terrestrial plant larch suggests that Flavobacteriia are main catalysts of the rapid turnover of arabinogalactans in the marine environment.
Colocalization analysis of 3′ UTR alternative polyadenylation quantitative trait loci reveals novel mechanisms underlying associations with lung function
Authors: Saferali Aabida , Kim Wonji , Xu Zhonghui , Chase Robert P , Cho Michael H , Laederach Alain , Castaldi Peter J , Hersh Craig P 
Published: April 2024

Abstract
While many disease-associated single nucleotide polymorphisms (SNPs) are expression quantitative trait loci (eQTLs), a large proportion of genome-wide association study (GWAS) variants are of unknown function. Alternative polyadenylation (APA) plays an important role in posttranscriptional regulation by allowing genes to shorten or extend 3′ untranslated regions (UTRs). We hypothesized that genetic variants that affect APA in lung tissue may lend insight into the function of respiratory associated GWAS loci. We generated alternative polyadenylation (apa) QTLs using RNA sequencing and whole genome sequencing on 1241 subjects from the Lung Tissue Research Consortium (LTRC) as part of the NHLBI TOPMed project. We identified 56 179 APA sites corresponding to 13 582 unique genes after filtering out APA sites with low usage. We found that a total of 8831 APA sites were associated with at least one SNP with q-value &lt; 0.05. The genomic distribution of lead APA SNPs indicated that the majority are intronic variants (33%), followed by downstream gene variants (26%), 3′ UTR variants (17%), and upstream gene variants (within 1 kb region upstream of transcriptional start site, 10%). APA sites in 193 genes colocalized with GWAS data for at least one phenotype. Genes containing the top APA sites associated with GWAS variants include membrane associated ring-CH-type finger 2 (MARCHF2), nectin cell adhesion molecule 2 (NECTIN2), and butyrophilin subfamily 3 member A2 (BTN3A2). Overall, these findings suggest that APA may be an important mechanism for genetic variants in lung function and chronic obstructive pulmonary disease (COPD).
Technical note: utilization of various allotment strategies to evaluate variation and replications required to detect statistical significance in nursery pig research
Authors: Jenkins Abigail K , Bromm Jenna J , Gebhardt Jordan T , Woodworth Jason C , Goodband Robert D , Tokach Mike D , DeRouchey Joel M 
Published: April 2024

Abstract
A total of 720 barrows (line 200 × 400, DNA genetics) were used in two 42-d nursery trials (initially 6.20 ± 0.12 kg and 5.63 ± 0.16 kg, respectively) to evaluate strategies for allotting pigs to pens in randomized controlled trials. At placement, the population was split into three cohorts with similar average weight and standard deviation and randomly assigned to one of the three allotment strategies. Strategy 1 (random) utilized a simple randomization strategy with each pig randomized to pens independent of all other pigs. Strategy 2 (body weight [BW] distribution) sorted each pig within the cohort into one of the five BW groups. One pig from each weight group was then randomly assigned to a pen such that distribution of BW within pen was uniform across pens. Strategy 3 (BW grouping) sorted pigs within the cohort into 3 BW categories: light, medium, and heavy. Within each BW category, pigs were randomized to pen to create pens of pigs from each BW category. Within each experiment, there were 72 pens with five pigs per pen and 24 pens per allotment strategy. For all strategies, once pigs were allotted to pens, pens were allotted to one of the two treatments for a concurrent trial. In experiment 1, environmental enrichment using ropes tied near the pan of the feeder was compared to a control with no enrichment. In experiment 2, treatment diets consisted of basal levels of Zn and Cu from the trace mineral premix for the duration of the study (110 and 17 mg/kg, respectively; control), or diets (supplemented control) with carbadox (50 g/ton; Mecadox, Phibro Animal Health, Teaneck, NJ) fed in phase 1 (days 0 to 22) and 2 (days 22 to 43), pharmacological levels of Zn and Cu (2,414 mg/kg Zn from ZnO; 168 mg/kg Cu from CuSO4) fed in phase 1, and only pharmacological levels of Cu (168 mg/kg Cu from CuSO4) fed in phase 2. These treatment designs were used to determine the impact on coefficient of variation (CV) and to estimate the number of replications required to find significant treatment differences based on allotment strategy. There were no meaningful allotment strategy × treatment interactions for either study. For between-pen CV, pigs allotted using BW distribution and BW grouping strategies had the lowest CV at allotment and final weight in both trials. For overall average daily gain in experiments 1 and 2 in experiment 2, the BW distribution strategy required the fewest replications to detect differences in performance. However, there is no meaningful difference between allotment strategies in replications required to detect significant differences for gain:feed ratio.
Targeting fusion oncoproteins in childhood cancers: challenges and future opportunities for developing therapeutics
Authors: Verma Sharad K , Witkin Keren L , Sharman Anu , Smith Malcolm A 
Published: April 2024

Abstract
Fusion oncoproteins are associated with childhood cancers and have proven challenging to target, aside from those that include kinases. As part of its efforts for targeting childhood cancers, the National Cancer Institute recently conducted a series on Novel Chemical Approaches for Targeting Fusion Oncoproteins. Key learnings on leading platforms and technologies that can be used to advance the development of molecular therapeutics that target fusion oncoproteins in childhood cancers are described. Recent breakthroughs in medicinal chemistry and chemical biology provide new ground and creative strategies to exploit for the development of targeted agents for improving outcomes against these recalcitrant cancers.
Brazilian indigenous nonstarter lactic acid bacteria enhance the diversification of volatile compounds in short-aged cheese
Authors: Barreto Pinilla Cristian Mauricio , da Silva Oliveira Wellington , de Oliveira Garcia Aline , Spadoti Leila Maria , Redruello Begoña , del Rio Beatriz , Alvarez Miguel Angel , Torres Silva e Alves Adriana 
Published: April 2024

Abstract
There is growing interest in using autochthonous lactic acid bacteria (LAB) that provide unique sensory characteristics to dairy products without affecting their safety and quality. This work studied the capacity of three Brazilian indigenous nonstarter LABs (NSLAB) to produce biogenic amines (BAs) and evaluated their effect on the volatile organic compounds (VOCs), microbial LAB communities, and physicochemical profile of short-aged cheese. Initially, the strain's potential for biosynthesis of BAs was assessed by PCR and in vitro assays. Then, a pilot-scale cheese was produced, including the NSLAB, and the microbial and VOC profiles were analyzed after 25 and 45 days of ripening. As a results, the strains did not present genes related to relevant BAs and did not produce them in vitro. During cheese ripening, the Lactococci counts were reduced, probably in the production of alcohols and acid compounds by the NSLAB. Each strain produces a unique VOC profile that changes over the ripening time without the main VOCs related to rancid or old cheese. Particularly, the use of the strain Lacticaseibacillus. paracasei ItalPN16 resulted in production of ester compounds with fruity notes. Thus, indigenous NSLAB could be a valuable tool for the enhancement and diversification of flavor in short-aged cheese.
Advances in isolated phages that affect Ralstonia solanacearum and their application in the biocontrol of bacterial wilt in plants
Authors: Tang You , Zhou Moxi , Yang Chuyun , Liu Rong , Du Hongyi , Ma Ming 
Published: April 2024

Abstract
Bacterial wilt is a widespread and devastating disease that impacts the production of numerous crucial crops worldwide. The main causative agent of the disease is Ralstonia solanacearum. Due to the pathogen's broad host range and prolonged survival in the soil, it is challenging to control the disease with conventional strategies. Therefore, it is of great importance to develop effective alternative disease control strategies. In recent years, phage therapy has emerged as an environmentally friendly and sustainable biocontrol alternative, demonstrating significant potential in controlling this severe disease. This paper summarized basic information about isolated phages that infect R. solanacearum, and presented some examples of their application in the biocontrol of bacterial wilt. The risks of phage application and future prospect in this area were also discussed. Overall, R. solanacearum phages have been isolated from various regions and environments worldwide. These phages belong mainly to the Inoviridae, Autographiviridae, Peduoviridae, and Cystoviridae families, with some being unclassified. Studies on the application of these phages have demonstrated their ability to reduce pathogenicity of R. solanacearum through direct lysis or indirect alteration of the pathogen's physiological properties. These findings suggested bacteriophage is a promising tool for biocontrol of bacterial wilt in plants.
Carbon- and Oxygen-rich stars in MaStar: identification and classification
Authors: Hill Lewis , Maraston Claudia , Thomas Daniel , Yan Renbin , Chen Yanping , Stringfellow Guy S , Lane Richard R , Fernández-Trincado José G 
Published: April 2024

ABSTRACT
Carbon- and Oxygen-rich stars populating the Thermally Pulsing Asymptotic Giant Branch (TP-AGB) phase of stellar evolution are relevant contributors to the spectra of ∼1 Gyr old populations. Atmosphere models for these types are uncertain, due to complex molecules and mass-loss effects. Empirical spectra are then crucial, but samples are small due to the short (∼3 Myr) TP-AGB lifetime. Here we exploit the vastness of the MaNGA Stellar library MaStar (∼60 000 spectra) to identify C and O-rich type stars. We define an optical colour selection with cuts of (g − r) &gt; 2 and (g − i) &lt; 1.55(g − r) − 0.07, calibrated with known C- and O-rich spectra. This identifies C- and O-rich stars along clean, separated sequences. An analogue selection is found in V, R, I bands. Our equation identifies C-rich and O-rich spectra with predictive performance metric F1-scores of 0.72 and 0.74 (over 1), respectively. We finally identify 41 C- and 87 O-rich type AGB stars in MaStar, 5 and 49 of which do not have a SIMBAD counterpart. We also detect a sample of non-AGB, dwarf C-stars. We further design a fitting procedure to classify the spectra into broad spectral types, by using as fitting templates empirical C- and O-rich spectra. We find remarkably good fits for the majority of candidates and categorize them into C- and O-rich bins following existing classifications, which correlate to effective temperature. Our selection models can be applied to large photometric surveys (e.g. Euclid, Rubin). The classified spectra will facilitate future evolutionary population synthesis models.
Role of ionizing background and galactic feedback in the redshift space clustering of O vi absorbers in hydrodynamical simulations
Authors: Maitra Soumak , Mallik Sukanya , Srianand Raghunathan 
Published: April 2024

ABSTRACT
We explore the effect of ionizing ultraviolet background (UVB) on the redshift space clustering of low-z (z ≤ 0.5) O vi absorbers using the Sherwood simulations incorporating ‘WIND’ (i.e. outflows driven by stellar feedback)-only and ‘WIND + AGN (active galactic nucleus)’ feedback. These simulations show positive clustering signals up to a scale of 3 Mpc. We find that the effect of feedback is restricted to small scales (i.e. ≤2 Mpc or ≈200 km s−1 at z ∼ 0.3) and ‘WIND’-only simulations produce a stronger clustering signal compared to simulations incorporating ‘WIND + AGN’ feedback. How the clustering signal is affected by the assumed UVB depends on the feedback processes assumed. For the simulations considered here, the effect of the UVB is confined to even smaller scales (i.e. &lt;1 Mpc or ≈100 km s−1 at z ∼ 0.3). These scales are also affected by exclusion caused by line blending. Therefore, our study suggests that clustering at intermediate scales (i.e. 1–2 Mpc for simulations considered here) together with the observed column density distribution can be used to constrain the effect of feedback in simulations.
GeV γ-ray emission in the field of young massive star cluster RCW 38
Authors: Ge Ting-Ting , Sun Xiao-Na , Yang Rui-Zhi , Tam Pak-Hin Thomas , Lu Ming-Xuan , Liang En-Wei 
Published: April 2024

ABSTRACT
We report the detection of γ-ray emission by the Fermi Large Area Telescope towards the young massive star cluster RCW 38 in the 1–500 GeV photon energy range. We found spatially extended GeV emission towards the direction of RCW 38, which is best modelled by a Gaussian disc of 0.23° radius with a significance of the extension of ∼11.4σ. Furthermore, the spatial correlation with the ionized and molecular gas content favours the hadronic origin of the γ-ray emission. The γ-ray spectrum of RCW 38 has a relatively hard photon index of 2.44 ± 0.03, which is similar to other young massive star clusters. We argue that the diffuse GeV γ-ray emission in this region likely originates from the interaction of accelerated protons in the stellar cluster with the ambient gas.
Post-AGB candidate IRAS 02143+5852: Cepheid-like variability, three-layer circumstellar dust envelope and spectral features
Authors: Ikonnikova N P , Burlak M A , Dodin A V , Shugarov S Yu , Belinski A A , Fedoteva A A , Tatarnikov A M , Rudy R J , Perry R B , Zheltoukhov S G , Atapin K E 
Published: April 2024

ABSTRACT
We present the results of multicolour UBVRCICJHK photometry, spectroscopic analysis and spectral energy distribution (SED) modelling for the post-AGB candidate IRAS 02143+5852. We detected Cepheid-like light variations with the full peak-to-peak amplitude ΔV ∼ 0.9 mag and the pulsation period of about 24.9 d. The phased light curves appeared typical for the W Vir Cepheids. The period–luminosity relation for the Type II Cepheids yielded the luminosity logL/L⊙ ∼ 2.95. From a low-resolution spectrum, obtained at maximum brightness, the following atmospheric parameters were determined: Teff ∼ 7400 K and logg ∼ 1.38. This spectrum contains the emission lines H α, ${\rm Ba\, {\small II}}$ λ6496.9, ${\rm He\, {\small I}}$λ10830, and Pa β. Spectral monitoring performed in 2019–2021 showed a significant change in the H α profile and appearance of CH and CN molecular bands with pulsation phase. The metal lines are weak. Unlike typical W Vir variables, the star shows a strong excess of infrared radiation associated with the presence of a heavy dust envelope around the star. We modelled the SED using our photometry and archival data from different catalogues and determined the parameters of the circumstellar dust envelope. We conclude that IRAS 02143+5852 is a low-luminosity analogue of dusty RV Tau stars.