bluuebunny/crossref_metadata_2025_split huggingface dataset:
Viable Vitreous Grafts of Whole Porcine Menisci for Transplant in the Knee and Temporomandibular Joints
Authors: Wang Shangping , Mueller Dustin , Chen Peng , Pan Ge , Wilson Marshall , Sun Shuchun , Chen Zhenzhen , Lee Thomas , Damon Brooke , Hepfer R. Glenn , Hill Cherice , Kern Michael J. , Pullen William M. , Wu Yongren , Brockbank Kelvin G. M. , Yao Hai 
Published: March 2024

AbstractThe shortage of suitable donor meniscus grafts from the knee and temporomandibular joint (TMJ) impedes treatments for millions of patients. Vitrification offers a promising solution by transitioning these tissues into a vitreous state at cryogenic temperatures, protecting them from ice crystal damage using high concentrations of cryoprotectant agents (CPAs). However, vitrification's success is hindered for larger tissues (>3 mL) due to challenges in CPA penetration. Dense avascular meniscus tissues require extended CPA exposure for adequate penetration; however, prolonged exposure becomes cytotoxic. Balancing penetration and reducing cell toxicity is required. To overcome this hurdle, a simulation‐based optimization approach is developed by combining computational modeling with microcomputed tomography (µCT) imaging to predict 3D CPA distributions within tissues over time accurately. This approach minimizes CPA exposure time, resulting in 85% viability in 4‐mL meniscal specimens, 70% in 10‐mL whole knee menisci, and 85% in 15‐mL whole TMJ menisci (i.e., TMJ disc) post‐vitrification, outperforming slow‐freezing methods (20%–40%), in a pig model. The extracellular matrix (ECM) structure and biomechanical strength of vitreous tissues remain largely intact. Vitreous meniscus grafts demonstrate clinical‐level viability (≥70%), closely resembling the material properties of native tissues, with long‐term availability for transplantation. The enhanced vitrification technology opens new possibilities for other avascular grafts.
Self‐reported history of breastfeeding in relation to recalled age at menarche in the United States
Authors: Gold Claire L. , Bertone‐Johnson Elizabeth 
Published: March 2024

AbstractMenarche is a key life history event that shapes the female reproductive trajectory and is important to the study of human biology because of the associated epidemiological and social consequences later in life. Our question is whether breastfeeding is associated with the variation in timing of menarche. Using data from a college‐aged female student population from Amherst, MA, we examined whether having been breastfed was associated with age at menarche. Of the 340 female participants with information on breastfeeding during infancy, we found that women who were breastfed (n = 286) had an adjusted mean age of menarche of 12.53 years (SE 0.09), while those who were not breastfed (n = 54) had an adjusted mean age of menarche of 12.04 years (SE 0.20; p < 0.03). We propose further research that explores a finer distinction between formula‐fed, mixed‐fed or predominantly breastfed infants, duration of breastfeeding and age at menarche.
Bridging Atom Engineering for Low‐Temperature Oxygen Activation in a Robust Metal–Organic Framework
Authors: Wang Rui , Wang Zi‐Yu , Zhang Yuan , Shaheer A. R. Mahammed , Liu Tian‐Fu , Cao Rong 
Published: March 2024

AbstractAchieving active site engineering at the atomic level poses a significant challenge in the design and optimization of catalysts for energy‐efficient catalytic processes, especially for a reaction with two reactants competitively absorbed on catalytic active sites. Herein, we show an example that tailoring the local environment of cobalt sites in a robust metal–organic framework through substituting the bridging atom from −Cl to −OH group leads to a highly active catalyst for oxygen activation in an oxidation reaction. Comprehensive characterizations reveal that this variation imparts drastic changes on the electronic structure of metal centers, the competitive reactant adsorption behavior, and the intermediate formation. As a result, exceptional low‐temperature CO oxidation performance was achieved with T25(Temperature for 25 % conversion)=35 °C and T100 (Temperature for 100 % conversion)=150 °C, which stands out from existing MOF‐based catalysts and even rivals many noble metal catalysts. This work provides a guidance for the rational design of catalysts for efficient oxygen activation for an oxidation reaction.
Nickel‐Catalyzed Regio‐ and Enantioselective Migratory Hydrocyanation of Internal Alkenes: Expanding the Scope to α,ω‐Diaryl Internal Alkenes
Authors: Jiao Mingdong , Long Jinguo , Chen Jianxi , Yang Hua , Wang Ting , Fang Xianjie 
Published: March 2024

AbstractMetal‐hydride‐catalyzed migratory functionalization of alkenes witnessed extensive development in the past few years. However, the asymmetric version of this reaction has remained largely underdeveloped owing to the difficulty in simultaneous control of both regio‐ and stereoselectivity. In addition, exploring the wider alkene substrate scope to enable more synthetically valuable applications represents another challenge in this field. In this context, a nickel‐catalyzed asymmetric hydrocyanation of internal alkenes involving a chain‐walking process is demonstrated. The reaction exhibits excellent regio‐ and enantioselectivity, proceeds under mild reaction conditions, and delivers benzylic nitriles in high yields. Even α,ω‐diaryl internal alkenes, which are known to be one of the most challenging substrates of this type, could be successfully converted to the desired products with good regio‐ and stereoselectivity by modifying the electronic and steric effects. Theoretical calculations suggest that the η3‐benzyl coordination mode and the aryl substituent (3,5‐(OMe)2C6H3) on the diphosphite ligand are both key factors in regulating regio‐ and enantioselectivity.
NIR‐II Absorption/Fluorescence of D–A π‐Conjugated Polymers Composed of Strong Electron Acceptors Based on Boron‐Fused Azobenzene Complexes
Authors: Nakamura Masashi , Kanetani Ippei , Gon Masayuki , Tanaka Kazuo 
Published: March 2024

AbstractLuminescence in the second near‐infrared (NIR‐II, 1,000–1,700 nm) window is beneficial especially for deep tissue imaging and optical sensors because of intrinsic high permeability through various media. Strong electron‐acceptors with low‐lying lowest unoccupied molecular orbital (LUMO) energy levels are a crucial unit for donor–acceptor (D–A) π‐conjugated polymers (CPs) with the NIR‐II emission property, however, limited kinds of molecular skeletons are still available. Herein, D–A CPs involving fluorinated boron‐fused azobenzene complexes (BAz) with enhanced electron‐accepting properties are reported. Combination of fluorination at the azobenzene ligand and trifluoromethylation at the boron can effectively lower the LUMO energy level down to −4.42 eV, which is much lower than those of conventional strong electron‐acceptors. The synthesized series of CPs showed excellent absorption/fluorescence property in solution over a wide NIR range including NIR‐II. Furthermore, owing to the inherent solid‐state emissive property of the BAz skeleton, obvious NIR‐II fluorescence from the film (up to λFL=1213 nm) and the nanoparticle in water (λFL=1036 nm, brightness=up to 29 cm−1 M−1) were observed, proposing that our materials are applicable for developing next‐generation of NIR‐II luminescent materials.
Mechanical, viscoelastic, and biodegradability characteristics of ramie fibre‐reinforced acrylonitrile butadiene styrene composites
Authors: Behera Basanta Kumar 
Published: March 2024

AbstractBiocomposites made from natural fibers have attracted significant attention in recent years owing to the global concern regarding plastic accumulation. In this investigation, ramie fiber was employed as a natural fiber reinforcement in the acrylonitrile butadiene styrene (ABS) matrix to enhance the biodegradability of the polymer composite. ABS/ramie fiber composites were prepared by compression molding with various wt% (5, 10, 15, and 20 wt %) of ramie fibers within the ABS matrix. Subsequently, the biodegradability, water absorption, mechanical properties, viscoelastic behavior, and thermal characteristics of the prepared composites were evaluated. The biodegradation test results demonstrated that there was a positive correlation between the concentrations of fibers in neat ABS. The maximum biodegradation of the composites was 2.56% at 20 wt% ramie fibers in the ABS matrix. Moreover, an increase in the wt% of ramie fiber led to an increase in water absorption. Although the tensile strength and impact strength decreased with increasing fiber content, the tensile modulus of the ABS/ramie fiber composites initially increased up to 5 wt% fiber and then started decreasing, and the natural fibers in ABS reduced the viscoelastic properties. The objective of developing ABS/ramie fiber composites is to utilize them as environmentally sustainable materials in automotive manufacturing industry.
Evolution of staining methods in neuroanatomy: Impetus for emanation of neuron doctrine during the turn of 20th century
Authors: Ghosh Sanjib Kumar , Walocha Jerzy A. 
Published: March 2024

AbstractThe nervous system is distinctive as compared to other tissue systems in human body owing to intricate structural organization. Histological studies played a key role in unveiling complex details of nervous tissue. However, the process of developing suitable staining method for nerve cells was arduous and spanned across almost half a century. The present study explored details of the journey involving quest for propitious staining method in neuroanatomy culminating in promulgation of neuron doctrine at the onset of 20th century. Initial efforts involving hematoxylin (including its diverse modifications) and subsequent adoption of analogous dye‐based stains (like Nissl's method) had limited success in visualization of different parts of a nerve cell and structural details of nervous tissue. This was due to inability of dye‐based stains to penetrate the connective tissue sheath of nervous tissue. Eventually, advent of metallic stains in form of silver impregnation method (Golgi stain), reduced silver impregnation method with gold stain (Cajal's stain) and silver carbonate staining method of Río‐Hortega unraveled the structure of nervous tissue. The evolution of staining methods catalyzed the refinement of theories pertinent to constitution of nervous tissue. Golgi's staining led to emergence of reticular theory (neurons exist as a network) and Nissl's staining was the basis of the concept of Nervösen Grau (nerve cells and glial cells are embedded in mass of gray matter). Finally, Cajal's staining method successfully elucidated the complex anatomy of nerve terminals and resulted in emanation of neuron doctrine (neurons exists as individual units with adjacent connections).
An empirical exploration of the subjectivity problem of information qualities
Authors: Van der Sluis Frans , Faure Julien , Homnual Sofie Phutachard 
Published: March 2024

AbstractInformation qualities such as usefulness, accuracy, and comprehensiveness are to some extent subjective. Information resources have different meanings to different people and at different moments. This apparent subjectivity hinders indexing based on qualities for retrieval and filtering purposes. We conceptualize this as the subjectivity problem and address it through two studies. Study One explores whether, on public fora, people consider qualities as claims they should agree upon. Study Two explores, through a vignettes study, which conditions foster this inter‐subjective validity of quality claims. We conclude that information qualities become agreeable given the right set of conditions. We discuss the need for transparency about information qualities and quality considerations in order to offer these conditions to end users.
Sn‐MFI and Fe‐MFI zeolites for fructose conversion to levulinic and lactic acids by the one‐pot method
Authors: Sobuś Natalia , Król Magdalena , Drożdż Ewa , Piotrowski Marcin , Yoney Büşra , Kornaus Kamil , Komarek Sebastian 
Published: March 2024

AbstractThis paper presents the results of the use of MFI zeolite as a catalyst modified with tin and iron. Sn‐MFI and Fe‐MFI catalysts were obtained by ion exchange under hydrothermal conditions with and without ammonium exchange. Catalytic materials were characterized with the use of analytical techniques such as X‐ray diffraction (XRD), Brunauer–Emmett–Teller method (BET), diffuse reflectance spectroscopy in the ultraviolet‐visible range (DRS UV–visible), hydrogen temperature programmed reduction (H2‐TPR), or Fourier transform infrared (FTIR) spectroscopy. The one‐pot catalytic conversion of fructose was performed at 220 °C for 1–5 h. Based on the results, the influence of time and material selection on the products obtained can be seen. Lactic acid (LAC) was obtained with a yield of 68.7% (after 2 h) and levulinic acid (LA) with a yield of 70.9% (after 5 h) with the participation of MFI. In turn, formic acid with a yield of 28.5% (after 5 h) was obtained with the participation of Fe‐MFI.
Kinetic and quantitative analysis of gaseous products of thermochemical biomass conversion based on thermogravimetric and mass‐spectrometric data
Authors: Badenko Vladislav , Sosnovsky Ilya , Kozlov Alexander , Penzik Maxim , Zhang Shu 
Published: March 2024

AbstractThe aim of this study is to apply a new approach to the quantitative analysis of the formation of gaseous products generated during the thermochemical conversion of wood waste samples of different fineness by using thermogravimetric analysis with mass spectrometry (TGA‐MS). Profiles of the release of gaseous components in the process of thermochemical conversion of wood samples were obtained based on data from the combined use of mass spectrometry and thermogravimetry. The resulting mass spectrometric data were processed according to the proposed methodology and the characteristics of the released gases were noted. The kinetic coefficients of the gases’ formation were calculated from the processed data.
Teacher perspectives on the introduction of linguistics in the languages classroom: Evidence from a co‐creation project on French, German and Spanish
Authors: Sheehan Michelle , Havinga Anna D. , Kasstan Jonathan R. , Stollhans Sascha , Corr Alice , Gillman Peter 
Published: March 2024

AbstractLinguistics is conspicuously absent from language teaching in UK schools. A‐level cultural topics cover a range of themes such as cyber‐society, cultural heritage and multiculturalism, but the approach taken to these topics is not informed by linguistics. In previous work, we have argued that this is an unfortunate omission not only because linguistics is appealing to many language students and perceived by them to be useful, but also because the existing cultural topics could be significantly enriched by the inclusion of the critical/analytical study of language itself. In this paper, we provide concrete examples of how linguistics can be integrated into the existing A‐level curriculum for Modern Foreign Languages (MFL) in England and Wales. Reporting on a project in which teachers trialled linguistics materials co‐created by us (a group of academics) and experienced languages teachers, we present evidence that linguistics materials are perceived to be both highly novel and nonetheless compatible with the existing A‐level curriculum. Data from questionnaires and semi‐structured interviews with participating teachers also show that: (i) these new materials can be taught with little or no prior experience of linguistics; and (ii) adding linguistics materials to the curriculum leads to significant impacts on teacher and pupil attitudes towards language(s). Despite some challenges, which we also discuss, the results highlight again the great potential of linguistics as a component of language teaching and the contribution that it can make to the enrichment of the discipline.
Patterns of B‐cell lymphocyte expression changes in pre‐ and post‐malignant prostate tissue are associated with prostate cancer progression
Authors: Sadasivan Sudha M. , Loveless Ian M. , Chen Yalei , Gupta Nilesh S. , Sanii Ryan , Bobbitt Kevin R. , Chitale Dhananjay A. , Williamson Sean R. , Rundle Andrew G. , Rybicki Benjamin A. 
Published: March 2024

AbstractBackroundInflammation characterized by the presence of T and B cells is often observed in prostate cancer, but it is unclear how T‐ and B‐cell levels change during carcinogenesis and whether such changes influence disease progression.MethodsThe study used a retrospective sample of 73 prostate cancer cases (45 whites and 28 African Americans) that underwent surgery as their primary treatment and had a benign prostate biopsy at least 1 year before diagnosis. CD3+, CD4+, and CD20+ lymphocytes were quantified by immunohistochemistry in paired pre‐ and post‐diagnostic benign prostate biopsy and tumor surgical specimens, respectively. Clusters of similar trends of expression across two different timepoints and three distinct prostate regions—benign biopsy glands (BBG), tumor‐adjacent benign glands (TAG), and malignant tumor glandular (MTG) regions—were identified using Time‐series Anytime Density Peaks Clustering (TADPole). A Cox proportional hazards model was used to estimate the hazard ratio (HR) of time to biochemical recurrence associated with region‐specific lymphocyte counts and regional trends.ResultsThe risk of biochemical recurrence was significantly reduced in men with an elevated CD20+ count in TAG (HR = 0.81, p = 0.01) after adjusting for covariates. Four distinct patterns of expression change across the BBG‐TAG‐MTG regions were identified for each marker. For CD20+, men with low expression in BBG and higher expression in TAG compared to MTG had an adjusted HR of 3.06 (p = 0.03) compared to the reference group that had nominal differences in CD20+ expression across all three regions. The two CD3+ expression patterns that featured lower CD3+ expression in the BBG compared to the TAG and MTG regions had elevated HRs ranging from 3.03 to 4.82 but did not reach statistical significance.ConclusionsLongitudinal and spatial expression patterns of both CD3+ and CD20+ suggest that increased expression in benign glands during prostate carcinogenesis is associated with an aggressive disease course.
Modified coronally advanced flap technique in the treatment of fibrous epulis: A case report with 1‐year follow‐up
Authors: Zheng Kaixin , Zhang Yuan 
Published: March 2024

AbstractBackgroundCurrently, the treatment of epulis is primarily surgical excision, which would greatly affect the aesthetics of patients if happened in the anterior region. It's challenging for clinicians to balance the aesthetic after surgery and less surgical trauma. To overcome this disadvantage, the authors propose the modified coronally advanced flap technique which applies the principles of minimally invasive surgery to provide satisfactory therapeutic results in fibrous epulis.MethodsWe report a case of an 18‐year‐old female with the chief complaint of a gingival swelling in the right upper anterior region. After the initial periodontal therapy, the modified surgical approach was applied to this patient. Unlike conventional coronally advanced flap technique, an additional incision was made, and the free portion was rotated into the adjacent space to completely cover the trauma, which avoided the use of the second operative zone.ResultsThe gingiva recovered with normal color, contour, and consistency after surgery, the papilla filled up the proximal space well and was in good harmony with the adjacent papillae. The surgical results remained stable during the follow‐up period.ConclusionsThe use of modified coronally advanced flap technique allows the clinician to successfully resume the natural appearance of gingiva in the treatment of fibrous epulis, as well as simplify the surgical approach, shorten the operative time, and demonstrate no tendency of recurrence.Key PointsWhy is this case new information?

This novel technique not only removes the epulis, but also takes into account the postoperative aesthetics of the surgery at the same time.
This minimally invasive surgical technique reduces operative time and increases patient comfort.
Keys to successful management of this case are as follows: (i) Adequate preoperative assessment of the location of the additional incision; (ii) tension‐free coronal flap advancement.What are the primary limitations to success in this case?

Clinical studies with long‐term outcomes of this approach are needed.
This procedure may be limited to larger gingival tumors.
Neuromodulatory Effects Induced by the Association of Moringa oleifera Lam., Tribulus terrestris L., Rhodiola rosea Lam., and Undaria pinnatidifida Extracts in the Hypothalamus
Authors: Chiavaroli Annalisa , Di Simone Simonetta Cristina , Acquaviva Alessandra , Nilofar Nilofar , Libero Maria Loreta , Brunetti Luigi , Recinella Lucia , Leone Sheila , Orlando Giustino , Zengin Gokhan , Di Vito Maura , Menghini Luigi , Ferrante Claudio 
Published: March 2024

AbstractThe present study investigated the role of a commercial formulation constituted by herbal extracts from Rhodiola rosea, Undaria pinnatifida, Tribulus terrestris, and Moringa oleifera. The formulation was analysed for determining the content in total phenols and flavonoids and scavenging/reducing properties. The formulation was also tested on isolated mouse hypothalamus in order to investigate effects on serotonin, dopamine, neuropeptide Y (NPY), and orexin A. The gene expression of gonadrotopin releasing hormone (GnRH) was also assayed. The formulation was able to reduce dopamine and serotonin turnover, and this could be related, albeit partially, to the capability of different phytochemicals, among which hyperoside and catechin to inhibit monoaminooxidases activity. In parallel, the formulation was effective in reducing the gene expression of NPY and orexin‐A and to improve the gene expression of GnRH. In this context, the increased GnRH gene expression induced by the formulation may contribute not only to improve the resistance towards the stress related to ageing, but also to prevent the reduction of libido that could be related with a stimulation of the serotoninergic pathway. According to the in silico analysis, hyperoside could play a pivotal role in modulating the gene expression of GnRH. Regarding NPY and orexin A gene expression, no direct interactions between the formulation phytochemicals and these neuropeptides were anticipated; thus, suggesting that the pattern of gene expression observed following exposure of the hypothalamus to the formulation may be secondary to inhibitory effects of dopamine and serotonin turnover. Concluding, the present study demonstrated the efficacy of the formulation in exerting neuromodulatory effects at the hypothalamic level; thus, suggesting the potential to contrast stress and fatigue.
Exploring the Efficacy of Noncovalent SARS‐CoV‐2 Main Protease Inhibitors: A Computational Simulation Analysis Study
Authors: Xiong Fei , Zhang Yan‐Jun , Jiang Hui‐Ying , Wang Zhong‐Hua 
Published: March 2024

AbstractThe SARS‐CoV‐2 main protease, as a key target for antiviral therapeutics, is instrumental in maintaining virus stability, facilitating translation, and enabling the virus to evade innate immunity. Our research focused on designing non‐covalent inhibitors to counteract the action of this protease. Utilizing a 3D‐QSAR model and contour map, we successfully engineered eight novel non‐covalent inhibitors. Further evaluation and comparison of these novel compounds through methodologies including molecular docking, ADMET analysis, frontier molecular orbital studies, molecular dynamics simulations, and binding free energy revealed that the inhibitors N02 and N03 demonstrated superior research performance (N02 ΔGbind=−206.648 kJ/mol, N03 ΔGbind=−185.602 kJ/mol). These findings offer insightful guidance for the further refinement of molecular structures and the development of more efficacious inhibitors. Consequently, future investigations can draw upon these findings to unearth more potent inhibitors, thereby amplifying their impact in the treatment and prevention of associated diseases.