bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intra- and inter-individual variation of BIS-index® and Entropy® during controlled sedation with midazolam/remifentanil and dexmedetomidine/remifentanil in healthy volunteers: an interventional study
Authors: Haenggi Matthias , Ypparila-Wolters Heidi , Hauser Kathrin , Caviezel Claudio , Takala Jukka , Korhonen Ilkka , Jakob Stephan M 
Published: February 2009

Abstract
Introduction
We studied intra-individual and inter-individual variability of two online sedation monitors, BIS® and Entropy®, in volunteers under sedation.

Methods
Ten healthy volunteers were sedated in a stepwise manner with doses of either midazolam and remifentanil or dexmedetomidine and remifentanil. One week later the procedure was repeated with the remaining drug combination. The doses were adjusted to achieve three different sedation levels (Ramsay Scores 2, 3 and 4) and controlled by a computer-driven drug-delivery system to maintain stable plasma concentrations of the drugs. At each level of sedation, BIS® and Entropy® (response entropy and state entropy) values were recorded for 20 minutes. Baseline recordings were obtained before the sedative medications were administered.

Results
Both inter-individual and intra-individual variability increased as the sedation level deepened. Entropy® values showed greater variability than BIS® values, and the variability was greater during dexmedetomidine/remifentanil sedation than during midazolam/remifentanil sedation.

Conclusions
The large intra-individual and inter-individual variability of BIS® and Entropy® values in sedated volunteers makes the determination of sedation levels by processed electroencephalogram (EEG) variables impossible. Reports in the literature which draw conclusions based on processed EEG variables obtained from sedated intensive care unit (ICU) patients may be inaccurate due to this variability.

Trial registration
clinicaltrials.gov Nr. NCT00641563.
Commercialization, patenting and genomics: researcher perspectives
Authors: Murdoch CJ , Caulfield Timothy 
Published: February 2009

AbstractThe impact of commercialization and patenting pressure on genomics research is still a topic of considerable debate in academic, policy and popular literature. We interviewed genomic researchers to see if their perspectives offered fresh insights. Regional Genome Canada centers provided us with relevant researcher contact information, and in-depth structured interviews were conducted. Researcher perspectives were sharply divided, with both support and concern for commercialization regimes surfacing in interviews. Data withholding and publication delays were commonly reported, but the aggressive enforcement of patents was not. There are parallels to the Stem Cell community in Canada in these respects. Genomic researchers, as individuals directly implicated in the field of controversy, have developed varied and often novel insights which should be incorporated into the ongoing debates surrounding commercialization and patenting. Many researchers continue to raise concerns, particularly in relation to data withholding, thus emphasizing the need for a continued exploration of the complex issues associated with commercialization and patenting.
A Summary of Deliberations on Strategic Planning for Continuous Quality Improvement in Laboratory Medicine
Authors: Grzybicki Dana Marie , Shahangian Shahram , Pollock Anne M. , Raab Stephen S. 
Published: February 2009

Abstract
On September 24–26, 2007, the Centers for Disease Control and Prevention convened the 2007 Institute on Critical Issues in Health Laboratory Practice: Managing for Better Health to develop an action plan for change for the immediate and long-term future. A wide variety of stakeholders, including pathologists, pathologist extenders, clinicians, and researchers, examined means to improve laboratory service communication, quality parameters, and potential future laboratory contributions to health care. In this summary document, we present the identified gaps, barriers, and proposed action plans for improvement for laboratory medicine in the 6 quality domains identified by the Institute of Medicine: safety, effectiveness, patient centeredness, timeliness, efficiency, and equity. Five major recommendations emerged from concluding discussions and included focusing on preanalytic and postanalytic processes as areas of potential quality improvement and recruiting a multidisciplinary group of nonlaboratory stakeholders to work with laboratory personnel to achieve improvement goals.
Challenges and Pitfalls of Morphologic Identification of Fungal Infections in Histologic and Cytologic Specimens
Authors: Sangoi Ankur R. , Rogers William M. , Longacre Teri A. , Montoya Jose G. , Baron Ellen Jo , Banaei Niaz 
Published: February 2009

Abstract
Despite the advantages of providing an early presumptive diagnosis, fungal classification by histopathology can be difficult and may lead to diagnostic error. To assess the accuracy of histologic diagnosis of fungal infections vs culture (“gold standard”), we performed a 10-year retrospective review at our institution. Of the 47 of 338 positive mold and yeast cultures with concurrent surgical pathology evaluation without known history of a fungal infection, 37 (79%) were correctly identified based on morphologic features in histologic and/or cytologic specimens. The 10 discrepant diagnoses (21%) included misidentification of septate and nonseptate hyphal organisms and yeast forms. Errors resulted from morphologic mimics, use of inappropriate terminology, and incomplete knowledge in mycology. The accuracy did not correlate with preceding antifungal therapy (P = .14) or use of special stains (P = .34) and was not operator-dependent. Among 8 discrepancies with clinical follow-up available, 2 potential adverse clinical consequences resulted. While histopathologic identification of fungi in tissue sections and cytologic preparations is prone to error, implementation of a standardized reporting format should improve diagnostic accuracy and prevent adverse outcomes.
Flow Cytometry Can Diagnose Classical Hodgkin Lymphoma in Lymph Nodes With High Sensitivity and Specificity
Authors: Fromm Jonathan R. , Thomas Anju , Wood Brent L. 
Published: February 2009

Abstract
The diagnosis of classical Hodgkin lymphoma (CHL) has been made in tissue sections as attempts to identify neoplastic Hodgkin and Reed Sternberg (HRS) cells in lymph nodes by flow cytometry (FC) have been unsuccessful. However, we have recently demonstrated that HRS cells can be identified by FC, often present as T-cell–HRS-cell rosettes. In this study, we examined the usefulness of a novel 9-color (CD95–Pacific blue/CD64–fluorescein isothiocyanate/CD30-phycoerythrin [PE]/CD45-PE–Texas red/CD40–PE cyanine [Cy]5.5/CD20-PECy7/CD15-allophycocyanin [APC]/CD71-APC–AlexaFluor A700/CD5-APC-Cy7), single tube FC assay to diagnose CHL in lymph nodes. We used the FC assay to determine diagnostic sensitivity and specificity in 279 blindly identified and 141 selected (for specimen type or cytopreparation morphologic features suggesting CHL) tissues. Of the 53 morphologically defined CHL cases identified (10 in the unselected group; 43 in the selected group), the FC assay diagnostic sensitivity and specificity were 88.7% and 100%, respectively. With the current availability of 8 (or more) color clinical flow cytometers, this assay can now be applied to routinely immunophenotype and confirm a diagnosis of CHL or as an adjunct to immunohistochemical analysis.
Activating children's thinking skills (ACTS): The effects of an infusion approach to teaching thinking in primary schools
Authors: Dewey Jessica , Bento Janet 
Published: February 2009

BackgroundRecent interest in the teaching of thinking skills within education has led to an increase in thinking skills packages available to schools. However many of these are not based on scientific evaluation (DfEE, 1999). This paper endeavours to examine the effectiveness of one approach, that of infusion, to teaching thinking.AimsTo investigate the impact of an infusion methodology, activating children's thinking skills (ACTS), on the cognitive, social, and emotional development of children in Year 4–6 in primary schools. This is a sister project to research being conducted in Northern Ireland (McGuinness, 2006).SampleThe study involved 404 children from 8 primary schools in one local authority. These were divided into 160 in the experimental group and 244 in the waiting list control group.MethodsA quasi‐experimental design was used with pre‐, post‐, and delayed post‐tests to ascertain changes in children's cognitive abilities, self‐perceptions, and social/behavioural skills using quantitative measures. In addition qualitative techniques were used with pupils and teachers to evaluate effectiveness.ResultsThe experimental group made significantly greater gains in cognitive ability skills over a 2 year period compared to the waiting list control. Qualitative data demonstrated a positive impact on children's social and emotional development. In addition teacher professional development was reported to be enhanced.ConclusionsThis research indicated that children's cognitive abilities can be developed following a 2 year period of the ACTS infusion intervention. While some positive effects were evidenced on the social and emotional development of children, further study will be necessary to examine these in more detail.
Estimation of Subgrade Soils Resilient Modulus from in-situ Devices Test Results
Authors: Mohammad Louay N. , Nazzal Munir D. , Abu-Farsakh Murad Y. , Alshibli Khalid 
Published: February 2009

Abstract
Field and laboratory testing programs were conducted to develop resilient modulus prediction models for application in the design and evaluation procedures of pavement structures. The field testing program included conducting several in-situ tests such as Geogauge, Light Falling Weight Deflectometer, and Dynamic Cone Penetrometer (DCP). The laboratory program consisted of performing repeated load triaxial resilient modulus tests, physical properties, and compaction tests on soil samples obtained from tested sections. A total of four subgrade soil types at different moisture-dry unit weight levels were considered. Comprehensive statistical analyses were conducted on the field and laboratory test results. Two sets of models were developed. The first set (direct model) directly relates the laboratory measured resilient modulus values with the results of each of the three in-situ devices, whereas the second set (soil property model) incorporates soil properties in addition to the results of each of the three in-situ devices. A good agreement was observed between the predicted and measured values of the resilient modulus. Furthermore, the results showed that the resilient modulus prediction was enhanced when the soil properties were included as variables within the models. Among the models developed, the DCP-soil property model had the best prediction of resilient modulus followed by the DCP-direct model. The effectiveness of the DCP models were further evaluated during a forensic analysis of pavement section failure in a highway within Louisiana.
Community assembly and shifts in plant trait distributions across an environmental gradient in coastal California
Authors: Cornwell William K. , Ackerly David D. 
Published: February 2009

Community assembly processes are thought to shape the mean, spread, and spacing of functional trait values within communities. Two broad categories of assembly processes have been proposed: first, a habitat filter that restricts the range of viable strategies and second, a partitioning of microsites and/or resources that leads to a limit to the similarity of coexisting species. The strength of both processes may be dependent on conditions at a particular site and may change along an abiotic gradient.We sampled environmental variables and plant communities in 44 plots across the varied topography of a coastal California landscape. We characterized 14 leaf, stem, and root traits for 54 woody plant species, including detailed intraspecific data for two traits with the goal of understanding the connection between traits and assembly processes in a variety of environmental conditions.We examined the within‐community mean, range, variance, kurtosis, and other measures of spacing of trait values. In this landscape, there was a topographically mediated gradient in water availability. Across this gradient we observed strong shifts in both the plot‐level mean trait values and the variation in trait values within communities. Trends in trait means with the environment were due largely to species turnover, with intraspecific shifts playing a smaller role. Traits associated with a vertical partitioning of light showed a greater range and variance on the wet soils, while nitrogen per area, which is associated with water use efficiency, showed a greater spread on the dry soils.We found strong nonrandom patterns in the trait distributions consistent with expectations based on trait‐mediated community assembly. There was a significant reduction in the range of six out of 11 leaf and stem functional trait values relative to a null model. For specific leaf area (SLA) we found a significant even spacing of trait values relative to the null model. For seed size we found a more platykurtic distribution than expected. These results suggest that both a habitat filter and a limit to the similarity of coexisting species can simultaneously shape the distribution of traits and the assembly of local plant communities.
Community‐level density dependence: an example from a shallow coral assemblage
Authors: Tanner Jason E. , Hughes Terence P. , Connell Joseph H. 
Published: February 2009

While density dependence is a popular topic of research in population ecology, it has received much less attention at the community level. Using 27 years of data from Heron Island, on Australia's Great Barrier Reef, we develop a matrix model of coral community dynamics that shows that community‐level density dependence does occur and that it is fairly common, being found in 38% of the model parameters for which it was tested. In particular, colonization of free space (through either recruitment or growth of existing colonies) was nearly always density dependent. There were no consistent patterns in the results for mortality, persistence, or species interactions. Most transitions were found to be dependent on the cover of the incoming species group, with only a few dependent on that of the outgoing species group. In addition, few of the transitions representing species interactions were dependent on the amount of free space present, suggesting that the cover of other species does not influence encounters.When these results were combined into a model of community dynamics, it was found that density dependence resulted in a moderate increase in coral cover, which was spread over most species groups. The dynamics of the density‐dependent assemblage were also a lot noisier than those of an assemblage without density dependence. Sensitivity analysis indicated that it was density dependence in the colonization probabilities, particularly of encrusting acroporids, bushyAcroporaand staghornAcropora, which had the main influence on the model, although persistence of free space was also important. Transitions representing mortality were only of minor importance, and those representing species interactions were of no importance.
An ecological risk assessment for triclosan in the terrestrial environment
Authors: Reiss Richard , Lewis Gavin , Griffin John 
Published: February 2009

AbstractTriclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) is a broad-spectrum bactericide used throughout North America and Europe for a variety of antimicrobial functions. This paper addresses the risk to terrestrial organisms from several potential exposure pathways: Exposure experienced by earthworms, terrestrial plants, and soil microorganisms as the result of the use of sewage sludge containing triclosan as an agricultural soil amendment; secondary exposure by birds and mammals from consumption of earthworms that have been exposed to triclosan in soil; and secondary exposure by birds and mammals from consumption of fish exposed to triclosan as the result of wastewater treatment discharges. The assessment found satisfactory margins of safety for plants, earthworms, birds, fish, mammals, and soil microorganisms. The lowest margins of safety were for nontarget plants (100 for the typical scenario and 8 for the upper-bound scenario). However, these margins of safety are still above the European Union (EU) recommended fivefold assessment value for nontarget plants and are based on cucumber results from a vegetative vigor study conducted in quartz sand that is of limited relevance for risk assessment. In a pre-emergence study conducted in a more relevant soil (sandy loam), cucumbers showed no response to triclosan at the highest dose tested (1,000 μg/kg). A recent study provides limited field measurements of soil and earthworm concentrations. While that study finds higher soil and earthworm concentrations than were estimated in the present study, even these higher concentrations do not indicate significant risks.
Effect-directed analysis of sediment-associated algal toxicants at selected hot spots in the River Elbe basin with a special focus on bioaccessibility
Authors: Schwab Katrin , Altenburger Rolf , Varel Urte Lübcke-von , Streck GEORG , Brack Werner 
Published: February 2009

AbstractBenthic algae are the dominant primary producers in many rivers and may be at risk of being affected by sediment-associated toxicants. Toxicity as well as bioaccessibility should be considered for the identification of major toxicants affecting benthic algae. Thus, effect-directed analysis (EDA) combining biotesting, fractionation, and chemical analysis was combined with both exhaustive and bioaccessibility-directed extraction to identify the major toxicants in sediments at three industrial sites in the Elbe River basin (Bitterfeld, Most, and Přelouč). Exhaustive accelerated solvent extraction was used together with bioaccessibility-directed extraction with TENAX® in aqueous suspension. Growth inhibition of the green alga Scenedesmus vacuolatus identified polycyclic aromatic compounds as important algal toxicants at all three sites, while in Bitterfeld and Přelouč additional toxicants, such as N-phenyl-2-naphthylamine and 7H-benzo[de]anthracen-7-one, played an important role. Confirmation of the identified compounds was based on the index of confirmation quality (ICQ) comparing the effect of an artificial mixture to that of the original sample. The results suggest a successful confirmation of identified toxicants for most fractions with ICQ values between 0.5 and 2. The bioaccessibility of toxicants decreased in the order Přelouč &gt; Bitterfeld &gt; Most as determined by the investigation of TENAX samples. Similar total concentrations of toxicants in Přelouč and Most sediment result in bioaccessible concentrations in Přelouč that exceed those in Most by a factor of 10 to 20. The extraction with TENAX with a subsequent EDA proved to be a powerful tool for prioritizing contaminants and contaminated sites with respect to the hazard of sediment-associated toxicants to green algae.
Palaeobiogeography of early planktonic foraminifera
Authors: Hudson Wendy , Hart Malcolm B. , Smart Christopher W. 
Published: February 2009

AbstractOver the last 50 years, our knowledge of early planktonic foraminifera has changed markedly. In 1958 Grigelis described “Globigerina oxfordiana” from the Upper Jurassic of Lithuania and this has, subsequently, become identified as one of the most geographically widespread of Jurassic planktonic taxa. There is a danger in that many authors identify any planktonic foraminiferid from the Jurassic as this taxon, rather than consider the alternative species described in the literature. In the period from 1967 to 1973, however, Dr W. Fuchs (Austria) identified a number of new taxa from the Triassic and Jurassic of Austria and the Jurassic of Poland and claimed that the history of planktonic foraminifera began in the Triassic. Following a long re-investigation of all his material in the Geologische Bundesanstalt in Vienna by the authors it appears that one of his new genera, Praegubkinella, was probably the ancestor of Conoglobigerina in the Toarcian (Early Jurassic). The first conoglobigerinids have been described from Toarcian – Aalenian – Bajocian strata in Central and Eastern Europe (including parts of the Former Soviet Union). The palaeobiogeography of Conoglobigerina and other related genera such as Globuligerina, Haeuslerina and Compactogerina are discussed and the problems yet to be resolved (e.g., records of “planktonic” taxa in the Triassic/Jurassic offshore N.W. Australia) are documented. The distribution (and evolution) of the Jurassic and Early Cretaceous planktonic foraminifera appear to be related to sea level highstands and continental fragmentation, especially in the earliest Cretaceous.
Biogeographical affinities of Late Cretaceous continental tetrapods of Europe: a review
Authors: Pereda-Suberbiola Xabier 
Published: February 2009

AbstractThe continental tetrapod assemblages from the Santonian-Maastrichtian of Europe consist of dinosaurs (theropods: Abelisauroidea, Alvarezsauridae, Dromaeosauridae, ?Oviraptorosauria, ?Troodontidae, and birds: Enantiornithes, basal Ornithurae; sauropods: Titanosauria; ankylosaurs: Nodosauridae; ornithopods: Hadrosauridae, Rhabdodontidae; and neoceratopsians), pterosaurs (Azhdarchidae), crocodyliforms (eusuchians: Alligatoroidea, Gavialoidea, ?Hylaeochampsidae; sebecosuchian-like ziphosuchians; and, probably, basal neosuchians), choristoderes (?Champsosauridae), squamates (lacertilians: Iguanidae s.l., Paramacellodidae, Polyglyphanodontinae, Varanoidea; snakes: Madtsoiidae; possible amphisbaenians), turtles (cryptodires: Chelydroidea, Kallokibotionidae, Solemydidae; pleurodires: Bothremydidae, Dortokidae), lissamphibians (Albanerpetontidae; anurans: Discoglossidae, Palaeobatrachidae; caudates: Batrachosauroididae, Salamandridae), and mammals (multituberculates: Kogaionidae, ?“Paracimexomys group”; marsupials: Herpetotheriidae; eutherians: “Zhelestidae”).The palaeobiogeographical affinities of the Late Cretaceous continental tetrapods of Europe are complex. The faunas are commonly considered to show a mixed pattern resulting from the addition of “Asiamerican” and Gondwanan forms to European taxa. Albanerpetontids, both paramacellodid and polyglyphonodontine lizards, and hadrosaurid dinosaurs are taxa with Palaeolaurasian (or, in some case, even Neopangean) affinities. Other forms, such as paleobatrachid and batrachosauroidid lissamphibians, solemydid turtles, alligatoroid crocodyliforms, and nodosaurid dinosaurs can be considered as Euramerican taxa. Kallokibotionid and dortokid turtles, rhabdodontid dinosaurs and kogaionid mammals are endemic to Europe. The Gondwanan taxa have been regarded as African immigrants that reached southern Europe via the Mediterranean Tethyan sill. Abelisaurid and titanosaurid dinosaurs, trematochampsid crocodyliforms, podocnemidid and bothremydid turtles, and boid and madtsoiid snakes constitute the basic pattern of the so-called “Eurogondwanan fauna”. However, the validity of some of these taxa is a disputed matter (Titanosauridae, Trematochampsidae), and the presence of other taxa in the Late Cretaceous of Europe is based on controversial data (Boidae, Podocnemididae). Only Abelisauroidea, Madtsoiidae and Bothremydidae (and, yet for confirming, Sebecosuchia) provide evidence of interchanges between Africa and Europe. At least abelisauroids might have reached Europe before the Late Cretaceous. In conclusion, most of the continental tetrapod taxa from the latest Cretaceous of Europe show affinities with those of northern Hemisphere landmasses. Latest Cretaceous trans-Tethyan dispersal events between Africa and Europe remain poorly documented.