bluuebunny/crossref_metadata_2025_split huggingface dataset:
With or without you: Family and Career‐Work in a Demanding and Precarious Profession
Authors: Reid Erin , Ghaedipour Farnaz , Obodaru Otilia 
Published: April 2024

AbstractFamily arrangements are crucial to people's abilities to meet the high demands of professional careers; but most scholarship has examined stable, highly remunerated professions. To understand the relationship between career and family within the increasing number of precarious professions, we analyse interviews with 102 journalists. We discover two broad types of career‐work practices these professionals employ to engage family in their careers: career‐family positioning (i.e., crafting a narrative of how career and family relate) and career‐family resourcing (i.e., generating resources from family for career or vice‐versa). Together, these practices touch more family members – spouses, children, parents, siblings, and extended family members – and involve a wider range of resources than documented in stable fields. By piecing together variations of these practices, professionals construct career strategies that address their difficult context in different ways. Two strategies largely accept the demands and precarity, by prioritizing career and drawing on family, or prioritizing career and forgoing family. A third, prioritizing family over career, involves defying the demands. Gender does not clearly influence which career strategy people pursue. These findings advance scholarship on career and family in the professions, social‐symbolic work, and contribute to careers research more broadly.
Unlocking liver health: Can tackling myosteatosis spark remission in metabolic dysfunction‐associated steatotic liver disease?
Authors: Henin Guillaume , Loumaye Audrey , Deldicque Louise , Leclercq Isabelle A. , Lanthier Nicolas 
Published: April 2024

AbstractMyosteatosis is highly prevalent in metabolic dysfunction‐associated steatotic liver disease (MASLD) and could reciprocally impact liver function. Decreasing muscle fat could be indirectly hepatoprotective in MASLD. We conducted a review to identify interventions reducing myosteatosis and their impact on liver function. Non‐pharmacological interventions included diet (caloric restriction or lipid enrichment), bariatric surgery and physical activity. Caloric restriction in humans achieving a mean weight loss of 3% only reduces muscle fat. Lipid‐enriched diet increases liver fat in human with no impact on muscle fat, except sphingomyelin‐enriched diet which reduces both lipid contents exclusively in pre‐clinical studies. Bariatric surgery, hybrid training (resistance exercise and electric stimulation) or whole‐body vibration in human decrease both liver and muscle fat. Physical activity impacts both phenotypes by reducing local and systemic inflammation, enhancing insulin sensitivity and modulating the expression of key mediators of the muscle‐liver‐adipose tissue axis. The combination of diet and physical activity acts synergistically in liver, muscle and white adipose tissue, and further decrease muscle and liver fat. Several pharmacological interventions (patchouli alcohol, KBP‐089, 2,4‐dinitrophenol methyl ether, adipoRon and atglistatin) and food supplementation (vitamin D or resveratrol) improve liver and muscle phenotypes in pre‐clinical studies by increasing fatty acid oxidation and anti‐inflammatory properties. These interventions are effective in reducing myosteatosis in MASLD while addressing the liver disease itself. This review supports that disturbances in inter‐organ crosstalk are key pathophysiological mechanisms involved in MASLD and myosteatosis pathogenesis. Focusing on the skeletal muscle might offer new therapeutic strategies to treat MASLD by modulating the interactions between liver and muscles.
Environmental DNA: The next chapter
Authors: Blackman Rosetta , Couton Marjorie , Keck François , Kirschner Dominik , Carraro Luca , Cereghetti Eva , Perrelet Kilian , Bossart Raphael , Brantschen Jeanine , Zhang Yan , Altermatt Florian 
Published: April 2024

AbstractMolecular tools are an indispensable part of ecology and biodiversity sciences and implemented across all biomes. About a decade ago, the use and implementation of environmental DNA (eDNA) to detect biodiversity signals extracted from environmental samples opened new avenues of research. Initial eDNA research focused on understanding population dynamics of target species. Its scope thereafter broadened, uncovering previously unrecorded biodiversity via metabarcoding in both well‐studied and understudied ecosystems across all taxonomic groups. The application of eDNA rapidly became an established part of biodiversity research, and a research field by its own. Here, we revisit key expectations made in a land‐mark special issue on eDNA in Molecular Ecology in 2012 to frame the development in six key areas: (1) sample collection, (2) primer development, (3) biomonitoring, (4) quantification, (5) behaviour of DNA in the environment and (6) reference database development. We pinpoint the success of eDNA, yet also discuss shortfalls and expectations not met, highlighting areas of research priority and identify the unexpected developments. In parallel, our retrospective couples a screening of the peer‐reviewed literature with a survey of eDNA users including academics, end‐users and commercial providers, in which we address the priority areas to focus research efforts to advance the field of eDNA. With the rapid and ever‐increasing pace of new technical advances, the future of eDNA looks bright, yet successful applications and best practices must become more interdisciplinary to reach its full potential. Our retrospect gives the tools and expectations towards concretely moving the field forward.
Demand and distribution in a dynamic spatial panel model for the United States: Evidence from state‐level data
Authors: Lima Gilberto Tadeu , Marques André M. 
Published: April 2024

AbstractWe estimate a modified demand‐and‐distribution system for the 48 contiguous US states and the District of Columbia (DC) employing dynamic spatial panel data for 1980–2019. We allow for endogenous regressors, test for the presence, significance, and magnitude of spatial spillovers, and estimate immediate and cumulative effects on our endogenous variables of interest. Without testing for spatial dependence and spillovers, we estimate that output growth and capacity utilization in the sample US states and DC rise in response to an increase in their own wage share. When we test for spatial dependence and spillovers as required by the state‐level nature of the data, and consider that the functional distribution of income and the level of economic activity are jointly determined, we estimate that a higher state wage share raises output growth in the own state and the neighboring states. Yet the effect of a change in the state wage share on capacity utilization in the own state and the neighboring states is not statistically significant. Meanwhile, a higher state output growth raises the wage share in the own state, but its impact on the wage share in the neighboring states is not statistically significant. A higher state capacity utilization raises the wage share in the own state, yet it reduces the wage share in the neighboring states.
The influence of viewing time on visual diagnostic accuracy: Less is more
Authors: Monteiro Sandra , Sherbino Jonathan , LoGiudice Andrew , Lee Mark , Norman Geoff , Sibbald Matthew 
Published: April 2024

AbstractBackgroundUnderstanding the factors that contribute to diagnostic errors is critical if we are to correct or prevent them. Some scholars influenced by the default interventionist dual‐process theory of cognition (dual‐process theory) emphasise a narrow focus on individual clinician's faulty reasoning as a significant contributor. In this paper, we examine the validity of claims that dual process theory is a key to error reduction.MethodsWe examined the relationship between a clinical experience (staff and resident physicians) and viewing time on accuracy for categorising chest X‐rays (CXRs) and electrocardiograms (ECGs). In two studies, participants categorised images as normal or abnormal, presented at viewing times of 175, 250, 500 and 1000 ms, to encourage System 1 processing. Study 2 extended viewing times to 1, 5, 10 and 20 s to allow time for System 2 processing and a diagnosis. Descriptives and repeated measures analysis of variance were used to analyse the proportion of true and false positive rates (TP and FP) as well as correct diagnoses.ResultsIn Study 1, physicians were able to detect abnormal CXRs (0.78) and ECGs (0.67) with relatively high accuracy. The effect of experience was found for ECGs only, as staff physicians (0.71, 95% CI = 0.66–0.75) had higher ECG TP than resident physicians (0.63, 95% CI = 0.58–0.68) in Study 1, and staff had lower ECG FP (0.10, 95% CI = 0.03–0.18) than resident physicians (0.27, 95% CI = 0.20–0.33) in Study 2. In other comparisons, experience was equivocal for ECG FPs and CXR TPs and FPs. In Study 2, overall diagnostic accuracy was similar for both ECGs and CXRs, (0.74). There were small interactions between experience and time for TP in ECGs and FP in CXRs, which are discussed further in the discussion and offer insights into the relationship between processing and experience.ConclusionOverall, our findings raise concerns about the practical application of models that link processing type to diagnostic error, or to specific diagnostic error reduction strategies.
Intermembrane space‐localized TbTim15 is an essential subunit of the single mitochondrial inner membrane protein translocase of trypanosomes
Authors: von Känel Corinne , Oeljeklaus Silke , Wenger Christoph , Stettler Philip , Harsman Anke , Warscheid Bettina , Schneider André 
Published: April 2024

AbstractAll mitochondria import >95% of their proteins from the cytosol. This process is mediated by protein translocases in the mitochondrial membranes, whose subunits are generally highly conserved. Most eukaryotes have two inner membrane protein translocases (TIMs) that are specialized to import either presequence‐containing or mitochondrial carrier proteins. In contrast, the parasitic protozoan Trypanosoma brucei has a single TIM complex consisting of one conserved and five unique subunits. Here, we identify candidates for new subunits of the TIM or the presequence translocase‐associated motor (PAM) using a protein–protein interaction network of previously characterized TIM and PAM subunits. This analysis reveals that the trypanosomal TIM complex contains an additional trypanosomatid‐specific subunit, designated TbTim15. TbTim15 is associated with the TIM complex, lacks transmembrane domains, and localizes to the intermembrane space. TbTim15 is essential for procyclic and bloodstream forms of trypanosomes. It contains two twin CX9C motifs and mediates import of both presequence‐containing and mitochondrial carrier proteins. While the precise function of TbTim15 in mitochondrial protein import is unknown, our results are consistent with the notion that it may function as an import receptor for the non‐canonical trypanosomal TIM complex.
Higher consumption of ultra‐processed foods is associated with obesity and abdominal obesity in socially vulnerable Brazilian women
Authors: de Medeiros Jocione Mara , Silva‐Neto Luiz Gonzaga Ribeiro , dos Santos Thays Lane Ferreira , dos Santos Neto João Eudes , de Menezes Toledo Florêncio Telma Maria 
Published: April 2024

AbstractThis study aimed to assess the consumption of ultra‐processed foods (UPF) and identify their association with obesity and abdominal obesity in adult women of reproductive age living in situations of social vulnerability in Maceió, Northeastern Brazil. This was a cross‐sectional study carried out between October 2020 and May 2021. An anthropometric evaluation was carried out to assess obesity and abdominal obesity. A dietary assessment was also conducted using a 24‐h food recall to determine the calorie intake from UPF. To estimate intra‐individual variability in food consumption, the probabilistic Multiple Source Method was used. These data in the form of tertiles were used to analyse the association between the consumption of UPF and obesity and abdominal obesity. Logistic regressions were used to analyse the association. A directed acyclic graph (DAG) was created for this analysis. This study included 1702 women of which 53.7% were 31 years old or older, and 74.2% lived in poverty. It identified that 36.5% and 38.1% of the women had obesity and abdominal obesity, respectively, and that an average of 33.8% of calories consumed came from UPF. In the analysis of association guided by the DAG, it was observed that women with a high‐calorie intake from UPF had a 1.3 times higher probability of being obese. It was also observed that women with a moderate and high‐calorie intake from UPF were 1.4 and 1.3 times more likely, respectively, to have abdominal obesity. Thus, it can be concluded that socially vulnerable women in Brazil have a relatively high consumption of UPF and that this condition increases the probability of obesity in this population group.
CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter
Authors: Wang Zhangqing , Zhang Yanting , Wu Yaru , Lai Duoduo , Deng Yuan , Ju Chuanfeng , Sun Lv , Huang Panpan , Wang Cun 
Published: April 2024

Summary

Boron (B) is crucial for plant growth and development. B deficiency can impair numerous physiological and metabolic processes, particularly in root development and pollen germination, seriously impeding crop growth and yield. However, the molecular mechanism underlying boron signal perception and signal transduction is rather limited.

In this study, we discovered that CPK10, a calcium‐dependent protein kinase in the CPK family, has the strongest interaction with the boron transporter BOR1. Mutations in CPK10 led to growth and root development defects under B‐deficiency conditions, while constitutively active CPK10 enhanced plant tolerance to B deficiency.

Furthermore, we found that CPK10 interacted with and phosphorylated BOR1 at the Ser689 residue. Through various biochemical analyses and complementation of B transport in yeast and plants, we revealed that Ser689 of BOR1 is important for its transport activity.

In summary, these findings highlight the significance of the CPK10‐BOR1 signaling pathway in maintaining B homeostasis in plants and provide targets for the genetic improvement of crop tolerance to B‐deficiency stress.
Transcriptomic features of programmed and inflammatory cell death in gingival tissues
Authors: Ebersole Jeffrey L. , Kirakodu Sreenatha S. , Nguyen Linh M. , Gonzalez Octavio A. 
Published: April 2024

AbstractThe local gingival tissue environment with homeostasis and tissue‐destructive events of periodontitis demonstrates major changes in histological features and biology of the oral/sulcular epithelium, fibroblasts, vascular cells, inflammatory cell infiltration, and alveolar bone.ObjectiveThis study used an experimental periodontitis model to detail the gingival transcriptome related to cell death processes of pyroptosis, necroptosis, ferroptosis, and cuproptosis.Materials and MethodsHealthy Macaca mulatta primates stratified by age, ≤3 years (young), 7–12 years (adolescent), 12–15 years (adult), and 17–23 years (aged), provided gingival tissue biopsies for microarray analysis focused on 257 genes representative of the four cell death processes and bacterial plaque samples for 16S rRNA gene analysis.ResultsAge differences in the profiles of gene expression in healthy tissues were noted for cuproptosis, ferroptosis, necroptosis, and pyroptosis. Major differences were then observed with disease initiation, progression, and resolution also related to the age of the animals. Distinct bacterial families/consortia of species were significantly related to the gene expression differences for the cell death pathways.ConclusionsThese results emphasized age‐associated differences in the gingival tissue molecular response to changes in the quality and quantity of bacteria accumulating with the disease process reflected in regulated cell death pathways that are both physiological and pathophysiological.
The roles of 5‐HT in orofacial pain
Authors: Zou Zhishan , Fan Wenguo , Liu Haotian , Liu Qing , He Hongwen , Huang Fang 
Published: April 2024

AbstractObjectivesAcute and chronic orofacial pain are very common and remain a vexing health problem that has a negative effect on the quality of life. Serotonin (5‐HydroxyTryptamine, 5‐HT) is a kind of monoamine neurotransmitter that is involved in many physiological and pathological processes. However, its role in orofacial pain remains inconclusive. Therefore, this review aims to summarize the recent advances in understanding the effect exerted by 5‐HT on the modulation of orofacial pain.Subjects and MethodsAn extensive search was conducted on PubMed and Web of Science for pertinent studies focusing on the effects of 5‐HT on the modulation of orofacial pain.ResultsIn this review, we concisely review how 5‐HT mediates orofacial pain, how 5‐HT is regulated and how we can translate these findings into clinical applications for the prevention and/or treatment of orofacial pain.Conclusions5‐HT plays a key role in the modulation of orofacial pain, implying that 5‐HT modulators may serve as effective treatment for orofacial pain. However, further research on the precise mechanisms underlying the modulation of orofacial pain is still warranted.
AN AEGEAN MIRROR FROM HALA SULTAN TEKKE, CYPRUS
Authors: Feldbacher Rainer , Alvarez Laura E. , Miyauchi Yuko , Lorentz Kirsi , Fischer Peter M. 
Published: April 2024

SummaryThis study deals with the results of the 2023 fieldwork at the extramural cemetery of the Late Bronze Age harbour city of Hala Sultan Tekke. One of the three excavated tombs in 2023 was the undisturbed Chamber Tomb XX, which is dated around 1300 BC. It contained a riveted bronze mirror, a rare type in Cyprus at that time, which is part of a mortuary context of four individuals out of a total of 17 individuals and 264 complete objects, many of them imported from a vast area, which includes the Mycenaean, Minoan, Egyptian and Levantine cultures. The current paper presents this mirror and associated contexts. As regards the provenance of the mirror, the Aegean is suggested as the area of manufacture, and more precisely Crete, suggesting potential evidence of direct contact between individuals from Crete and Hala Sultan Tekke.
Long‐term outcomes of two‐dose alemtuzumab induction in pediatric kidney transplantation
Authors: Engen Rachel M. , Bartosh Sharon M. 
Published: April 2024

AbstractBackgroundAlemtuzumab is a lymphocyte depleting agent used for induction in kidney transplant, but long‐term information on its use in pediatric recipients remains sparse.MethodsWe performed a single‐center retrospective cohort study of 57 pediatric kidney transplant recipients receiving alemtuzumab 20 mg/m2/dose ×2 doses for induction immunosuppression. All patients underwent surveillance biopsies, and 91.3% underwent steroid withdrawal by day 4 post‐transplant. Outcomes of interest included graft survival, development of donor specific antibodies (DSA), incidence of viremia and PTLD, and duration of lymphopenia.ResultsMedian follow‐up time was 7.9 years (IQR 5–13.6 years). Median graft survival was 16.5 years (95% CI 11.6‐unknown). DSA developed in 36.5% at a median of 944 days (IQR 252–2113 days). Incidences of BK polyomavirus DNAemia (BKPyV‐DNAemia), CMV DNAemia, and EBV DNAemia were 38.6%, 22.8%, and 14%, respectively; one patient developed PTLD at 13.3 years post‐transplant. Median duration of lymphopenia was 365 days (IQR 168–713 days); 19.3% of patients remained lymphopenic at 3 years post‐transplant. There was no association between duration of lymphopenia and graft survival, rejection, DSA detection, or viremia.ConclusionsA two‐dose alemtuzumab induction protocol can have excellent outcomes with a steroid‐free maintenance immunosuppression regimen. More comprehensive, multicenter, comparative studies of pediatric kidney transplant are needed to improve long‐term outcomes.
From intestinal failure to transplantation: Review on the current need for transplant indications under multidisciplinary transplant programs worldwide
Authors: Raghu Vikram K. , Rumbo Carolina , Horslen Simon P. 
Published: April 2024

AbstractIntroductionIntestinal failure, defined as the loss of gastrointestinal function to the point where nutrition cannot be maintained by enteral intake alone, presents numerous challenges in children, not least the timing of consideration of intestine transplantation.ObjectivesTo describe the evolution of care of infants and children with intestinal failure including parenteral nutrition, intestine transplantation, and contemporary intestinal failure care.MethodsThe review is based on the authors' experience supported by an in‐depth review of the published literature.ResultsThe history of parenteral nutrition, including out‐patient (home) administration, and intestine transplantation are reviewed along with the complications of intestinal failure that may become indications for consideration of intestine transplantation. Current management strategies for children with intestinal failure are discussed along with changes in need for intestine transplantation, recognizing the difficulty in generalizing recommendations due to the high level of heterogeneity of intestinal pathology and residual bowel anatomy and function.DiscussionAdvances in the medical and surgical care of children with intestinal failure have resulted in improved transplant‐free survival and a significant fall in demand for transplantation. Despite these improvements a number of children continue to fail rehabilitative care and require intestine transplantation as life‐saving therapy or when the burden on ongoing parenteral nutrition becomes too great to bear.
Successful use of eculizumab in immediate ANCA vasculitis recurrence in a pediatric kidney transplant
Authors: Peterson Caitlin G. , Jackson Rachel L. 
Published: April 2024

AbstractBackgroundKidney transplantation is an acceptable therapy end‐stage kidney disease secondary to antineutrophil cytoplasmic antibody‐associated vasculitis with risk of disease recurrence ranging from 3% to 17%. Standard posttransplant immunosuppression is the mainstay of therapy after recurrence. Recently, new medications focused on complement regulation and avoidance of steroids have been shown to be effective in treating antineutrophil cytoplasmic antibody (ANCA) vasculitis with no studies in the pediatric population.MethodsWe report a 5‐year‐old patient with immediate recurrence of positive myeloperoxidase (MPO)‐ANCA vasculitis after deceased donor kidney transplant and the novel use of eculizumab to salvage the graft.ResultsEculizumab and transition to ravulizumab has been successful in improving graft function and maintenance of disease remission after immediate MPO‐ANCA vasculitis recurrence posttransplant.ConclusionsComplement inhibitors may be used in addition to standard immunosuppression postkidney transplant in a pediatric patient with MPO‐ANCA vasculitis recurrence without higher rates of infections.