bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discrete dynamic Bayesian network analysis of fMRI data
Authors: Burge John , Lane Terran , Link Hamilton , Qiu Shibin , Clark Vincent P. 
Published: November 2007

AbstractWe examine the efficacy of using discrete Dynamic Bayesian Networks (dDBNs), a data‐driven modeling technique employed in machine learning, to identify functional correlations among neuroanatomical regions of interest. Unlike many neuroimaging analysis techniques, this method is not limited by linear and/or Gaussian noise assumptions. It achieves this by modeling the time series of neuroanatomical regions as discrete, as opposed to continuous, random variables with multinomial distributions. We demonstrated this method using an fMRI dataset collected from healthy and demented elderly subjects (Buckner, et al., 2000: J Cogn Neurosci 12:24‐34) and identify correlates based on a diagnosis of dementia. The results are validated in three ways. First, the elicited correlates are shown to be robust over leave‐one‐out cross‐validation and, via a Fourier bootstrapping method, that they were not likely due to random chance. Second, the dDBNs identified correlates that would be expected given the experimental paradigm. Third, the dDBN's ability to predict dementia is competitive with two commonly employed machine‐learning classifiers: the support vector machine and the Gaussian naïve Bayesian network. We also verify that the dDBN selects correlates based on non‐linear criteria. Finally, we provide a brief analysis of the correlates elicited from Buckner et al.'s data that suggests that demented elderly subjects have reduced involvement of entorhinal and occipital cortex and greater involvement of the parietal lobe and amygdala in brain activity compared with healthy elderly (as measured via functional correlations among BOLD measurements). Limitations and extensions to the dDBN method are discussed. Hum Brain Mapp, 2009. © 2007 Wiley‐Liss, Inc.
The influence of ageing on complex brain networks: A graph theoretical analysis
Authors: Micheloyannis Sifis , Vourkas Michael , Tsirka Vassiliki , Karakonstantaki Eleni , Kanatsouli Kassia , Stam Cornelis J. 
Published: November 2007

AbstractObjective:To determine the functional connectivity of different EEG bands at the “baseline” situation (rest) and during mathematical thinking in children and young adults to study the maturation effect on brain networks at rest and during a cognitive task.Methods:Twenty children (8–12 years) and twenty students (21–26 years) were studied. The synchronization likelihood was used to evaluate the interregional synchronization of different EEG frequency bands in children and adults, at rest and during math. Then, graphs were constructed and characterized in terms of local structure (clustering coefficient) and overall integration (path length) and the “optimal” organization of the connectivity i.e., the small world network (SWN).Results:The main findings were: (i) Enhanced synchronization for theta band during math more prominent in adults. (ii) Decrease of the optimal SWN organization of the alpha2 band during math. (iii) The beta and especially gamma bands showed lower synchronization and signs of lower SWN organization in both situations in adults.Conclusion:There are interesting findings related to the two age groups and the two situations. The theta band showed higher synchronization during math in adults as a result of higher capacity of the working memory in this age group. The alpha2 band showed some SWN disorganization during math, a process analog to the known desynchronization. In adults, a dramatic reduction of the connections in gray matter occurs. Although this maturation process is probably related to higher efficiency, reduced connectivity is expressed by lower synchronization and lower mean values of the graph parameters in adults. Hum Brain Mapp 2009. © 2007 Wiley‐Liss, Inc.
Do nutrition labels improve dietary outcomes?
Authors: Variyam Jayachandran N. 
Published: November 2007

AbstractThe disclosure of nutritional characteristics of most packaged foods became mandatory in the United States with the implementation of the Nutrition Labeling and Education Act (NLEA) in 1994. Under the NLEA regulations, a ‘Nutrition Facts’ panel displays information on nutrients such as calories, total and saturated fats, cholesterol, and sodium in a standardized format. By providing nutrition information in a credible, distinctive, and easy‐to‐read format, the new label was expected to help consumers choose healthier, more nutritious diets. This paper examines whether the disclosure of nutrition information through the mandatory labels impacted consumer diets. Assessing the dietary effects of labeling is problematic due to the confounding of the label effect with unobserved label user characteristics. This self‐selection problem is addressed by exploiting the fact that the NLEA exempts away‐from‐home foods from mandatory labeling. Difference‐in‐differences models that account for zero away‐from‐home intakes suggest that the labels increase fiber and iron intakes of label users compared with label nonusers. In comparison, a model that does not account for self‐selection implies significant label effects for all but two of the 13 nutrients that are listed on the label. Published in 2007 by John Wiley & Sons, Ltd.
Lack of functional erythropoietin receptors of cancer cell lines
Authors: Laugsch Magdalena , Metzen Eric , Svensson Tanja , Depping Reinhard , Jelkmann Wolfgang 
Published: November 2007

AbstractErythropoietin (Epo) therapy reduces red cell transfusion requirements and improves the quality of life of anemic cancer patients receiving chemotherapy. However, there is concern that Epo may promote tumor growth. We investigated by real‐time RT‐PCR, immunofluorescence microscopy, Western blotting and cell growth analysis whether human cancer cell lines (SH‐SY5Y, MCF7, HepG2, U2‐OS, HeLa, HEK293T, RCC4, HCT116, 7860wt and SW480) possess functional Epo receptors (EpoR). We detected EpoR mRNA in all cell lines. Neither hypoxia nor Epo treatment altered the level of EpoR mRNA expression. Four commonly used commercial antibodies proved to be unsuitable for immunoblot procedures because they cross‐reacted with several proteins unrelated with EpoR. Depending on the antibody used, EpoR was localized to the plasma membrane, the cytoplasm or the nucleus. Experiments with small interfering RNA showed that EpoR protein was not expressed by the tumor cells except by UT7/Epo leukemia cells, which served as an EpoR positive control line, and by cells transfected with the human EpoR gene. Apart from UT7/Epo, none of the tumor cell lines responded to Epo treatment with phosphorylation of signaling molecules or with cell proliferation. © 2007 Wiley‐Liss, Inc.
Trends in oesophageal cancer incidence and mortality in Europe
Authors: Bosetti Cristina , Levi Fabio , Ferlay Jacques , Garavello Werner , Lucchini Franca , Bertuccio Paola , Negri Eva , Vecchia Carlo La 
Published: November 2007

AbstractTo monitor recent trends in mortality from oesophageal cancer in 33 European countries, we analyzed the data provided by the World Health Organization over the last 2 decades, using also joinpoint regression. For selected European cancer registration areas, we also analyzed incidence rates for different histological types. For men in the European Union (EU), age‐standardized (world population) mortality rates were stable around 6/100,000 between the early 1980s and the early 1990s, and slightly declined in the last decade (5.4/100,000 in the early 2000s, annual percent change, APC = −1.1%). In several western European countries, male rates have started to level off or decline during the last decade (APC = −3.4% in France, and −3.0% in Italy). Also in Spain and the UK, which showed upward trends in the 1990s, the rates tended to level off in most recent years. A levelling of rates was observed only more recently in countries of central and eastern Europe, which had had substantial rises up to the late 1990s. Oesophageal cancer mortality rates remained comparatively low in European women, and overall EU female rates were stable around 1.1–1.2/100,000 over the last 2 decades (APC = −0.1%). In northern Europe a clear upward trend was observed in the incidence of oesophageal adenocarcinoma, and in Denmark and Scotland incidence of adenocarcinoma in men is now higher than that of squamous‐cell carcinoma. Squamous‐cell carcinoma remained the prevalent histological type in southern Europe. Changes in smoking habits and alcohol drinking for men, and perhaps nutrition, diet and physical activity for both sexes, can partly or largely explain these trends. © 2007 Wiley‐Liss, Inc.
The interactive relationship of competitive climate and trait competitiveness with workplace attitudes, stress, and performance
Authors: Fletcher Thomas D. , Major Debra A. , Davis Donald D. 
Published: November 2007

AbstractThere has been a considerable debate about the individual and organizational benefits of competition. Adopting a person–environment fit perspective, this research examined the influence of competition as an interaction between trait competitiveness and competitive climate. Using a sample of information technology workers, competitive climate was considered as both an individual level variable and a workgroup variable. Results show that the effect of competitive climate depended on trait competitiveness and the level at which climate was assessed for four of the outcomes assessed: job satisfaction, organizational commitment, job dedication, and supervisor‐rated task performance. In general, the effect of competitive climate was more negative for individuals lower in trait competitiveness. Competitive psychological climate was associated with greater stress regardless of the level of trait competitiveness but was not directly related to self‐rated task performance. Findings suggest that managers should be cautious in encouraging competitive climate. Copyright © 2007 John Wiley & Sons, Ltd.
Toward a unified theory of consumer acceptance technology
Authors: Kulviwat Songpol , Bruner II Gordon C. , Kumar Anand , Nasco Suzanne A. , Clark Terry 
Published: November 2007

AbstractIn the last few decades, scholars and practitioners have increasingly tried to understand the factors that influence technology acceptance. Theories and models developed by scholars have tended to focus on the role of cognition and have rarely included affect. The few studies that have incorporated affect have tended to measure a single emotion rather than modeling it comprehensively. This research addresses that inadequacy in our understanding of technology adoption by merging two previously unrelated models: TAM (the Technology Acceptance Model) and PAD (the Pleasure, Arousal, and Dominance paradigm of affect). This study also examines an enhanced view of cognition. The product of this unified theoretical framework is referred to as the Consumer Acceptance of Technology (CAT) model. The results of a test using structural equation modeling provide empirical support for the model. Overall, the CAT model explains over 50% of the variance in consumer adoption intentions, a considerable increase compared to TAM. These findings suggest that substantial improvement in the prediction of technology adoption decisions is possible by use of this model with its integration of affect and cognition. © 2007 Wiley Periodicals, Inc.
Tissue and organ donation for research in forensic pathology: the MRC Sudden Death Brain and Tissue Bank
Authors: Millar T , Walker R , Arango J‐C , Ironside JW , Harrison DJ , MacIntyre DJ , Blackwood D , Smith C , Bell JE 
Published: November 2007

AbstractNovel methodological approaches to the investigation of brain and non‐central nervous system disorders have led to increased demand for well‐characterized, high quality human tissue samples, particularly from control cases. In the setting of the new Human Tissue legislation, we sought to determine whether relatives who have been suddenly bereaved are willing to grant authorization for research use of post mortem tissue samples and organs in sufficient numbers to support the establishment of a brain and tissue bank based in the forensic service. Research authorization was sought from families on the day prior to forensic post mortem examination followed up by written confirmation. We have to date selected individuals who have died suddenly (age range 1–89 years) and who were likely to have normal brains or who had displayed symptoms of a CNS disorder of interest to researchers, including psychiatric disorders. One hundred and eleven families have been approached during the first 2 years of this project. Research use of tissue samples was authorized by 96% of families and 17% agreed to whole brain donation. Audit of families' experience does not suggest that they are further distressed by being approached. Respondents expressed a clear view that the opportunity for research donation should be open to all bereaved families. Despite the sometimes long post mortem intervals, the quality of tissue samples is good, as assessed by a range of markers including Agilent BioAnalyzer quantification of RNA integrity (mean value 6.4). We conclude that the vast majority of families are willing to support research use of post mortem tissues even in the context of sudden bereavement and despite previous adverse publicity. The potential for acquisition of normal CNS and non‐CNS tissues and of various hard‐to‐get CNS disorders suggests that efforts to access the forensic post mortem service for research material are eminently worthwhile. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Scaling‐up technique for net ecosystem productivity of deciduous broadleaved forests in Japan using MODIS data
Authors: Yamaji Takehiko , Sakai Toru , Endo Takahiro , Baruah Pranab J. , Akiyama Tsuyoshi , Saigusa Nobuko , Nakai Yuichiro , Kitamura Kenzo , Ishizuka Moriyoshi , Yasuoka Yoshifumi 
Published: November 2007

AbstractWe attempted to obtain carbon sequestration maps of deciduous forests in Japan using detectable parameters from the Moderate Resolution Imaging Spectrometer (MODIS) sensor and to determine how the spatial pattern of carbon sequestration differs within the same forest ecosystem type. For this investigation, we firstly parameterized the MODIS algorithm at one flux tower site, Takayama, for the years 2002–2003. The MODIS algorithm could link flux‐based net ecosystem productivity (NEP) with simple functions controlled by a thermal infrared band and a vegetation index. Second, the performance of the MODIS algorithm was validated through comparisons with the flux‐based NEP at another flux tower site, Hitsujigaoka. The MODIS‐based NEP at Hitsujigaoka was also within an accuracy of a flux‐based NEP with R2 of 0.879 and root mean square error of 1.64 gC m−2 day−1, regardless of canopy structure and age. The MODIS algorithm was noteworthy for its general applicability in different locations. Finally, we used the MODIS algorithm for the same forest ecosystem type in Japan for regional extrapolation of NEP. The MODIS‐based NEP of deciduous forests in Japan showed great variance with 347 ± 288 gC m−2 year−1 in 2002, according to the stand structure and climatic condition of the year. Studies for quantification of ecosystem carbon balance need to consider variance, frequency and spatial distributions of NEP. Satellite remote sensing demonstrated the potential for the large‐scale mapping of NEP.
Functional expression of a low-affinity zinc uptake transporter (FrZIP2) from pufferfish (Takifugu rubripes) in MDCK cells
Authors: Qiu Andong , Hogstrand Christer 
Published: November 2007

Zinc is a vital micronutrient to all organisms and it is therefore very important to determine the mechanisms that regulate cellular zinc uptake. Previously, we reported on zinc uptake transporters from zebrafish (Danio rerio; DrZIP1) and Fugu pufferfish (Takifugu rubripes; FrZIP1) that facilitated cellular zinc uptake of high affinity (Km&lt;0.5 μM) in both CHSE214 [chinook salmon (Oncorhynchus tshawytscha) embryonic 214] cells and Xenopus laevis oocytes. To investigate additional biochemical pathways of zinc uptake in fish, we molecularly cloned the second fish member (FrZIP2) of the SLC39 subfamily II from Fugu pufferfish gill. Functional characterization suggests that FrZIP2 stimulated zinc uptake in a temperature-, time-, concentration- and pH-dependent manner when overexpressed in MDCK cells (Madin–Darby canine kidney cells). In comparison with FrZIP1 and DrZIP1 (&lt;0.5 μM), FrZIP2 appears to represent a low-affinity zinc uptake transporter (Km=13.6 μM) in pufferfish. FrZIP2 protein was selective for zinc, but it might also transport Cu2+, since 20 times excess of Cu2+ completely abolished its zinc uptake activity. The zinc uptake by FrZIP2 was stimulated in a slightly acidic medium (pH 5.5–6.5) and was completely blocked at pH 7.5 and above, suggesting that an inward H+ gradient might provide a driving force for zinc transport by FrZIP2. Furthermore, FrZIP2-mediated zinc uptake activity was slightly inhibited by 0.5 mM HCO3−, indicating that FrZIP2 may employ a different mechanism of zinc translocation from the assumed HCO3−-coupled zinc transport used by human SLC39A2. The FrZIP2 gene was expressed in all the tissues studied herein, with especially high levels in the ovary and intestines. Thus FrZIP2 may be a prominent zinc uptake transporter of low affinity in many cell types of Fugu pufferfish.
Peroxisome-proliferator-activated receptors and the control of levels of prostaglandin-endoperoxide synthase 2 by arachidonic acid in the bovine uterus
Authors: Sheldrick E. Linda R. , Derecka Kamila , Marshall Elaine , Chin Evonne C. , Hodges Louise , Wathes D. Claire , Abayasekara D. Robert E. , Flint Anthony P. F. 
Published: November 2007

Arachidonic acid is a potential paracrine agent released by the uterine endometrial epithelium to induce PTGS2 [PG (prostaglandin)-endoperoxide synthase 2] in the stroma. In the present study, bovine endometrial stromal cells were used to determine whether PTGS2 is induced by arachidonic acid in stromal cells, and to investigate the potential role of PPARs (peroxisome-proliferator-activated receptors) in this effect. Arachidonic acid increased PTGS2 levels up to 7.5-fold within 6 h. The cells expressed PPARα and PPARδ (also known as PPARβ) (but not PPARγ). PTGS2 protein level was increased by PPAR agonists, including polyunsaturated fatty acids, synthetic PPAR ligands, PGA1 and NSAIDs (non-steroidal anti-inflammatory drugs) with a time course resembling that of arachidonic acid. Use of agonists and antagonists indicated PPARα (but not PPARδ or PPARγ) was responsible for PTGS2 induction. PTGS2 induction by arachidonic acid did not require PG synthesis. PTGS2 levels were increased by the PKC (protein kinase C) activators 4β-PMA and PGF2α, and the effects of arachidonic acid, NSAIDs, synthetic PPAR ligands and 4β-PMA were blocked by PKC inhibitors. This is consistent with PPAR phosphorylation by PKC. Induction of PTGS2 protein by 4β-PMA in the absence of a PPAR ligand was decreased by the NF-κB (nuclear factor κB) inhibitors MG132 and parthenolide, suggesting that PKC acted through NF-κB in addition to PPAR phosphorylation. Use of NF-κB inhibitors allowed the action of arachidonic acid as a PPAR agonist to be dissociated from an effect through PKC. The results are consistent with the hypothesis that arachidonic acid acts via PPARα to increase PTGS2 levels in bovine endometrial stromal cells.
Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm
Authors: Ono Nobuhito , Van der Heijden Ingrid , Scheffer George L. , Van de Wetering Koen , Van Deemter Elizabeth , De Haas Marcel , Boerke Arjan , Gadella Bart M. , De Rooij Dirk G. , Neefjes Jacques J. , Groothuis Tom A. M. , Oomen Lauran , Brocks Lenny , Ishikawa Toshihisa , Borst Piet 
Published: November 2007

The human and murine genes for MRP9 (multidrug resistance-associated protein 9; ABCC12) yield many alternatively spliced RNAs. Using a panel of monoclonal antibodies, we detected full-length Mrp9 only in testicular germ cells and mouse sperm; we obtained no evidence for the existence of the truncated 100 kDa MRP9 protein reported previously. In contrast with other MRPs, neither murine Mrp9 nor the human MRP9 produced in MRP9-transfected HEK-293 cells (human embryonic kidney cells) appears to contain N-linked carbohydrates. In mouse and boar sperm, Mrp9 localizes to the midpiece, a structure containing all sperm mitochondria. However, immunolocalization microscopy and cell fractionation studies with transfected HEK-293 cells and mouse testis show that MRP9/Mrp9 does not localize to mitochondria. In HEK-293 cells, it is predominantly localized in the endoplasmic reticulum. We have been unable to demonstrate transport by MRP9 of substrates transported by other MRPs, such as drug conjugates and other organic anions.
The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA
Authors: Clarke Thomas A. , Cole Jeffrey A. , Richardson David J. , Hemmings Andrew M. 
Published: November 2007

NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 Å (1 Å=0.1 nm) resolution. The architecture of the protein is that of a 40 Å ‘nanowire’ in which the five haems are positioned within 6 Å of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein–protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2–NrfB2 heterotetrameric complex.
Inhibition of MMP-2 gelatinolysis by targeting exodomain–substrate interactions
Authors: Xu Xiaoping , Chen Zhihua , Wang Yao , Bonewald Lynda , Steffensen Bjorn 
Published: November 2007

MMP-2 (matrix metalloproteinase 2) contains a CBD (collagen-binding domain), which is essential for positioning gelatin substrate molecules relative to the catalytic site for cleavage. Deletion of the CBD or disruption of CBD-mediated gelatin binding inhibits gelatinolysis by MMP-2. To identify CBD-binding sites on type I collagen and collagen peptides with the capacity to compete CBD binding of gelatin and thereby inhibit gelatinolysis by MMP-2, we screened a one-bead one-peptide combinatorial peptide library with recombinant CBD as bait. Analyses of sequences from the CBD-binding peptides pointed to residues 715–721 in human α1(I) collagen chain as a binding site for CBD. A peptide (P713) including this collagen segment was synthesized for analyses. In SPR (surface plasmon resonance) assays, the CBD and MMP-2E404A, a catalytically inactive MMP-2 mutant, both bound immobilized P713 in a concentration-dependent manner, but not a scrambled control peptide. Furthermore, P713 competed gelatin binding by the CBD and MMP-2E404A. In control assays, neither of the non-collagen binding alkylated CBD or MMP-2 with deletion of CBD (MMP-2ΔCBD) bound P713. Consistent with the exodomain functions of the CBD, P713 inhibited ∼90% of the MMP-2 gelatin cleavage, but less than 20% of the MMP-2 activity on a peptide substrate (NFF-1) which does not require the CBD for cleavage. Confirming the specificity of the inhibition, P713 did not alter MMP-2ΔCBD or MMP-8 activities. These experiments identified a CBD-binding site on type I collagen and demonstrated that a corresponding synthetic peptide can inhibit hydrolysis of type I and IV collagens by competing CBD-mediated gelatin binding to MMP-2.