bluuebunny/crossref_metadata_2025_split huggingface dataset:
Periplasmic carbonic anhydrase CAH1 contributes to high inorganic carbon affinity inChlamydomonas reinhardtii
Authors: Shimamura Daisuke , Ikeuchi Tomoaki , Tsuji Yoshinori , Fukuzawa Hideya , Yamano Takashi 
Published: March 2024

AbstractCarbonic anhydrase (CA), an enzyme conserved across species, is pivotal in the interconversion of inorganic carbon (Ci; CO2and HCO3−). Compared to the well-studied intracellular CA, the specific role of extracellular CA in photosynthetic organisms is still not well understood. In the green algaChlamydomonas reinhardtii, CAH1, located at the periplasmic space, is strongly induced under CO2-limiting conditions by the Myb transcription factor LCR1. While it has been observed that thelcr1mutant shows decreased Ci-affinity, the detailed mechanisms behind this phenomenon are yet to be elucidated. In this study, we aimed to unravel the LCR1-dependent genes essential for maintaining high Ci-affinity. To achieve this, we identified a total of 12 LCR1-dependent inducible genes under CO2-limiting conditions, focusing specifically on the most prominent ones -CAH1,LCI1,LCI6, andCre10.g426800. We then created mutants of these genes using the CRISPR-Cas9 system, all from the same parental strain, and compared their Ci-affinity. Contrary to earlier findings (Van and Spalding, 1999) that reported no reduction in Ci-affinity in thecah1mutant, our newly createdcah1-1 mutant exhibited a significant decrease in Ci-affinity under high HCO3−/CO2-ratio conditions. Additionally, when we treated wild-type cells with a CA inhibitor with low membrane permeability, a similar reduction in Ci-affinity was observed. Moreover, the addition of exogenous CA to thecah1mutant restored the decreased Ci-affinity. These results, highlighting the crucial function of the periplasmic CAH1 in maintaining high Ci-affinity inChlamydomonascells, provide new insights into the functions of periplasmic CA in algal carbon assimilation.One-sentence summaryCAH1, a periplasmic carbonic anhydrase inChlamydomonas reinhardtii, plays a crucial role in maintaining a high affinity for inorganic carbon, particularly under CO2-limiting conditions.
Comprehensive analysis of CXXX sequence space reveals thatS. cerevisiaeGGTase-I mainly relies on a2X substrate determinants
Authors: Sarkar Anushka , Hildebrandt Emily R. , Patel Khushi V. , Mai Emily T. , Shah Sumil S. , Kim June H. , Schmidt Walter K. 
Published: March 2024

AbstractMany proteins undergo a post-translational lipid attachment, which increases their hydrophobicity, thus strengthening their membrane association properties or aiding in protein interactions. Geranylgeranyltransferase-I (GGTase-I) is an enzyme involved in a three-step post-translational modification (PTM) pathway that attaches a 20-carbon lipid group called geranylgeranyl at the carboxy-terminal cysteine of proteins ending in a canonical CaaL motif (C - cysteine, a - aliphatic, L - often leucine, but can be phenylalanine, isoleucine, methionine, or valine). Genetic approaches involving two distinct reporters were employed in this study to assessS. cerevisiaeGGTase-I specificity, for which limited data exists, towards all 8000 CXXX combinations. Orthogonal biochemical analyses and structure-based alignments were also performed to better understand the features required for optimal target interaction. These approaches indicate that yeast GGTase-I best modifies the Cxa[L/F/I/M/V] sequence that resembles but is not an exact match for the canonical CaaL motif. We also observed that minor modification of non-canonical sequences is possible. A consistent feature associated with well-modified sequences was the presence of a non-polar a2residue and a hydrophobic terminal residue, which are features recognized by mammalian GGTase-I. These results thus support that mammalian and yeast GGTase-I exhibit considerable shared specificity.Article SummaryThis work investigates yeast GGTase-I specificity through genetics, high throughput sequencing, and two distinct reporter systems. This approach allows for comprehensive evaluation of all CXXX sequence space, which has not been possible with earlier approaches. We identified CXXX sequences supporting geranylgeranylation that differ from the historically defined CaaL sequence often cited in the literature as the GGTase-I target motif, and our results indicate that the last two amino acids of the target motif largely dictate GGTase-I specificity.
Contrasting patterns ofAsaiasp association withPlasmodium falciparumbetween field collectedAnopheles gambiaeandAnopheles coluzziifrom Cameroon
Authors: Maffo Claudine Grâce Tatsinkou , Sandeu Maurice Marcel , Tchoupo Micareme , Kamga Fleuriane Metissa Dondji , Mugenzi Leon M.J. , Njiokou Flobert , Hughes Grant L. , Wondji Charles S. 
Published: March 2024

AbstractThe presence of bacteria of the genusAsaiain mosquitoes makes them suitable candidates for malaria paratrangenic control. To better understand whether this bacterium could be used for malaria control, we investigated and quantifiedAsaiainfluence inAn.Lgambiae slpopulations naturally infected by the malaria parasitePlasmodium falciparum.AdultAnophelesmosquitoes were collected across two different eco-geographical localities in Cameroon, during the dry and wet seasons. DNA was extracted from the whole individual mosquitoes andAsaiawas quantified using real-time Q-PCR by amplification of 16S ribosomal RNA gene. We also detected and quantifiedPlasmodium falciparuminfection in same mosquitoes. The density ofAsaiawas quantified in 864 mosquitoes. This included 439An. gambiaecollected from Bankeng and 425An. coluzziicollected from Gounougou. The prevalence ofAsaiainAn. gambiaeandAn. coluzziiwas 88.3% and 80.9% respectively, withAn. gambiaewere more heavily infected byAsaiathanAn. coluzzii.There was also a significant difference between the infection densities ofAsaiaper season in the two localities. We also had a significant difference between the infection densities ofAsaiaaccording toPlasmodiuminfection status in the two localities. However, no correlation was observed between the amount ofAsaiaandP. falciparuminfection. This study provides evidence that naturally occurringAsaiainfection is not correlated toP. falciparumdevelopment withinAn. gambiaeandAn. coluzzii.Further studies as experimental infection and innate immune response are required to better investigate the correlation betweenAnophelesmosquitoes,AsaiaandPlasmodium.ImportanceMalaria control relies mainly on insecticide-based tools. However, the effectiveness of these tools is threatened by the widespread insecticide resistance in malaria vectors highlighting the need for alternative control approaches. The endosymbiontAsaiahas emerged as a promising candidate for paratransgenic control of malaria, but its biology and genetics still need to be further analyzed across Africa. Here, we investigated and quantifiedAsaiainfluence inAn.Lgambiae slpopulations naturally infected by the malaria parasitePlasmodium falciparum.DNA was extracted from the whole individual mosquitoes collected from two localities, and thenAsaiawas quantified using real-time Q-PCR by amplification of 16S ribosomal RNA gene. We also detected and quantifiedPlasmodium falciparuminfection in same mosquitoes and correlatedPlasmodiuminfection them with the presence ofAsaiainfection. This study provides evidence that naturally occurringAsaiainfection is not correlated toP. falciparumdevelopment withinAn. gambiaeandAn. coluzzii.
Pervasive mitochondrial tRNA gene loss in the clade B of haplosclerid sponges (Porifera, Demospongiae)
Authors: Lavrov Dennis V. , Turner Thomas L. , Vicente Jan 
Published: March 2024

AbstractMitochondrial tRNA gene loss and cytosolic tRNA import to mitochondria are two common phenomena in mitochondrial biology, but their importance is often under-appreciated in animals. This is because most bilaterally symmetrical animals (Bilateria) encode a complete set of tRNAs needed for mitochondrial translation. By contrast, studies of mitochondrial genomes in non-bilaterian animals have shown a reduced tRNA gene content in several lineages, necessitating tRNA import. Interestingly, in most of these lineages tRNA gene content appears to be set early in the evolution of the group and conserved thereafter. Here we demonstrate that Clade B of Haplosclerid Sponges (CBHS) represent an exception to this pattern. We determined mt-genome sequences for eight species from this group and analyzed them with six that had been previously available. In addition, we determined mt-genome sequences for two species of haploslerid sponges outside the CBHS and used them with eight previously available sequences as outgroups. We found that tRNA gene content varied widely among CBHS species: from three in an undescribedHaliclonaspecies (Haliclona sp. TLT785) to 25 inXestospongia mutaandX. testudinaria. Furthermore, we found that all CBHS species outside the genusXestospongialackedatp9, while some also lackedatp8. Analysis of nuclear sequences fromNiphates digitalisrevealed that bothatp8andatp9had transferred to the nuclear genome, while the absence of mt-tRNA genes represented their genuine loss. Overall, CBHS can be a useful animal system to study mt-tRNA genes loss, mitochondrial import of cytosolic tRNA, and the impact of both of these processes on mitochondrial evolution.Significance statementIt is generally believed that the gene content is stable in animal mitochondrial (mt) DNA. Indeed, mtDNA in most bilaterally symmetrical animals encompasses a conserved set of 37 genes coding for 13 proteins, two rRNAs and 22 tRNAs. By contrast, mtDNA in non-bilaterian animals shows more variation in mt gene content, in particular in the number of tRNA genes. However, most of this variation occurs between major non-bilaterian lineages. Here we demonstrate that a group of demosponges called Clade B of Haplosclerid Sponges (CBHS) represents a fascinating exception to this pattern, with species experiencing recurrent losses of up to 22 mt-tRNA genes. We argue that this group constitutes a promising system to investigate the effects of tRNA gene loss on evolution of mt-genomes as well as mitochondrial tRNA import machinery.
Multimessenger Constraints on Radiatively Decaying Axions from GW170817
Authors: Diamond M. , Fiorillo D. , Marques-Tavares G. , Tamborra I. , Vitagliano E. 
Published: March 2024

The metastable hypermassive neutron star produced in the coalescence of two neutron stars can copiously produce axions that radiatively decay into O(100)  MeV photons. These photons can form a fireball with characteristic temperature smaller than 1 MeV. By relying on x-ray observations of GW170817/GRB 170817A with CALET CGBM, Konus-Wind, and Insight-HXMT/HE, we present new bounds on the axion-photon coupling for axion masses in the range 1–400 MeV. We exclude couplings down to 5×10−11  GeV−1, complementing and surpassing existing constraints. Our approach can be extended to any feebly interacting particle decaying into photons.

Published by the American Physical Society
2024
Remote work’s impact on well-being: longitudinal analysis and the influence of gender, household size and childcare
Authors: Prati Gabriele 
Published: March 2024

PurposeThe aim of the study was to investigate the relationship between remote work and subjective well-being and the potential moderating role of gender, household size and childcare.Design/methodology/approachThe current research used data from the 2016 and 2020 editions of the Survey on Household Income and Wealth (SHIW) of the Bank of Italy. Italian workers were asked to report their subjective well-being and how many days per month they work remotely (at the time of the COVID-19 pandemic). Cross-sectional and longitudinal analyses were conducted on a sample of 1.103 and 184 participants, respectively.FindingsAfter controlling for the effects of gender, age, education and perceived economic condition, ordered probit models revealed that the relationship between remote work and subjective well-being was nonsignificant. Moreover, gender, household size and childcare did not play a moderating role in the relationship between remote work and subjective well-being.Practical implicationsThe findings of the current study suggest that an individualized approach is required to maximize the possible benefits of remote work.Originality/valueThe potential positive effects of remote work on subjective well-being might be overestimated.
Identifying and prioritizing coworking member needs
Authors: Johansson Erik , Rådman Erik , Raharjo Hendry , Bosch-Sijtsema Petra 
Published: March 2024

Purpose
This paper aims to identify and prioritize the needs of coworking members. The authors focus on maintaining the existing members rather than attracting new ones.

Design/methodology/approach
The authors use two phases and multiple methods. The first phase focuses on a qualitative approach using observations and interviews to uncover and formulate the members’ needs. The second phase focuses on prioritizing the needs using a quantitative approach.

Findings
The authors discovered 19 member needs from the coworking spaces. Based on an online survey, the authors classified those needs into three main Kano model’s categories.

Originality/value
The resulting member needs and their strategic priorities provide a useful basis for coworking providers to direct their improvement efforts towards achieving greater member satisfaction.
Shameful or shameless? Anxieties about mothers and women's autonomy on the Central African Copperbelt, 1956–1964
Authors: Lämmert Stephanie 
Published: March 2024

AbstractThis article deals with anxiety about and the shaming of modern urban mothers and wives on the mines of the late colonial Central African Copperbelt. Women's various labours and public presence lead to ambivalent depictions, such as the ‘careless mother’, that were part of a broader array of anxieties about women's autonomy on the mines. However, urban women rejected such labelling and resisted the isolation of modern motherhood. Instead, they created networks of mutual support and solidarity, they publicly shamed their failing breadwinners, supported one another financially, emotionally and through expressing solidarity in the press, thus seeking economic independence where possible.
Environmental ethics and green practices in the manufacturing sector: The role of green innovation and environmental policy
Authors: Ogiemwonyi Osarodion , Eneizan Bilal 
Published: March 2024

AbstractNumerous studies have indicated that incorporating an environmental ethic (EE), green innovation (GI), and environmental policy (EnP) can enhance a company's sustainable performance, that is, environmental performance (EP) and economic performance (EcP) in the manufacturing industry. However, to encourage eco‐friendly production and processes, it is crucial to promote sustainable practices across different industries. Nevertheless, some researchers have overlooked the significance of these implementations. Integrating these practices into the manufacturing sector can be challenging. To address this issue, this study has developed an empirical model that assesses two pillars of sustainable performance in the Jordanian manufacturing industry. The study analyzes the direct correlation between a firm's EE, GI, and sustainable performance. It also examines whether GI plays a mediating role in the relationship between company EE and sustainable performance and if EnP moderates the relationship between GI and sustainable performance. The study collected data from 386 respondents belonging to various manufacturing industries in Jordan and analyzed it using SmartPLS. The research revealed that a manufacturing firm's EE is significantly boosted by GI, EP, and EcP. These findings suggest that companies with higher EE are more likely to innovate sustainably and perform better in terms of sustainability. In particular, the study found that GI plays a mediating role between a firm's EE and its sustainable performance. Furthermore, the impact of EnP on the relationship between GI and EP was mixed, showing a moderating effect on the former but not on EcP. The study provides valuable insights into the implementation of green practices in the manufacturing sector and discusses policy implications for manufacturers.
Tracking floristic archetypes of Patagonian steppes
Authors: Easdale Marcos Horacio , Martin‐Albarracin Valeria Leticia , Perri Daiana Vanesa , López Dardo Rubén , Bruzzone Octavio Augusto 
Published: March 2024

AbstractQuestionsThe classification of plant communities is a well‐established practice in ecology. However, the factors that determine the distribution of common species and the extent of their dominance and overlap are still being debated. Large‐scale studies based on field data are of great interest in the face of biodiversity changes and climate change impacts. This research was designed to answer the following two questions: What are the dominant plant communities in the Patagonian steppes and how do they relate to environmental features, as measured by latitude, longitude, altitude, mean annual precipitation and mean temperature? What have been the recent changes in plant communities in terms of expansion and contraction of different types of communities?LocationPatagonian steppes (South America).MethodsWe used a large database of species‐by‐species vegetation cover from 426 monitoring plots in the South American Patagonian steppes, surveyed between 2007 and 2019, with two different assessment dates at least 5 years apart. We applied archetypal analysis to identify different vegetation compositions at a regional scale (called floristic archetypes) and to track their changes over time. In addition, we analyzed the relationship between floristic archetypes and spatial and meteorological variables to assess the association between floristic composition and the environmental context.ResultsSeven floristic archetypes were identified in the Patagonian steppes. The dominant and most widespread floristic archetype showed an expanding pattern in floristic trajectories and was positively associated with mean annual temperature. This floristic archetype was characterized by both drought‐ and grazing‐tolerant species. Two floristic archetypes located at the longitudinal extremes showed decreasing relative participation in the vegetation configurations, and the archetype located towards the west showed a positive relationship with annual precipitation, suggesting a threatened scenario in the face of drier conditions. Southern floristic archetypes either showed a slight decrease or remained stable.ConclusionsPatagonian steppes are dominated by both drought‐ and grazing‐tolerant species communities and their spatial distribution is expanding across different biomes, whereas the more forage‐preferred species were associated with a decreasing floristic archetype. A projected drier and hotter climate scenario may potentiate this pattern. The floristic archetypes framework has potential as a monitoring tool for tracking changes in vegetation composition at a mesoscale and for identifying hotspots of vulnerable vegetation in the face of environmental changes.
Cross‐notation knowledge of rational numbers predicts fraction arithmetic
Authors: Ching Boby Ho‐Hong , Li Xiang Yu , Chen Tiffany Ting 
Published: March 2024

AbstractBackgroundRecent research showed that cross‐notation magnitude knowledge of fractions and decimals was related to better performance in fraction arithmetic, but it remains unclear whether it made an independent contribution to fraction arithmetic longitudinally when other cognitive variables are considered.AimsTo examine the extent to which children's earlier knowledge of cross‐notation magnitude predicted subsequent performance in fraction addition and subtraction as well as fraction multiplication and division longitudinally.SampleThree hundred and fifty‐four Chinese children (Mage = 112.1 months).MethodsDuring the first wave of assessment, a range of cognitive abilities of children were measured, including within‐notation fraction and decimal magnitude comparisons, whole‐number arithmetic fluency, non‐verbal intelligence, attentive behaviours, counting recall, word‐level reading, and phonological awareness. Twelve months later, the same children were assessed again with two tasks of fraction arithmetic: fraction addition and subtraction as well as fraction multiplication and division.Results and ConclusionsMultiple linear regressions showed that within‐notation fraction and decimal magnitude knowledge predicted fraction addition and subtraction longitudinally, after the effects of working memory, nonverbal intelligence, language skills, attentive behaviour, and whole‐number arithmetic were controlled. Cross‐notation magnitude knowledge made independent contributions to fraction addition and subtraction longitudinally beyond the influence of within‐notation fraction and decimal magnitude knowledge and other covariates. However, within‐notation fraction and decimal magnitude knowledge were not associated with fraction multiplication and division, whereas cross‐notation magnitude knowledge remained a unique predictor. These findings suggest that it may be useful to incorporate cross‐notation knowledge in the assessments of children's mathematics abilities and teaching.
Complications of immuno‐oncology care: what urologist should know
Authors: Grajales Valentina , Martini Alberto , Shore Neal D. 
Published: March 2024

ObjectivesTo provide a practical review of immune‐related adverse events (irAEs) that may be encountered in uro‐oncology patients.Patients and MethodsWe conducted a literature review of studies reporting irAEs including articles published through September 2023 for uro‐oncology patients and the potential relevancy for the practicing urologist.ResultsImmunotherapy has revolutionised cancer treatment, extending its impact to urological malignancies including for patients with urothelial, kidney, and prostate cancers. Immuno‐oncology (IO) compounds have achieved measurable and durable responses in these cancers. Urologists, choosing to administer or co‐manage IO patient care, should be prepared to understand, evaluate, and treat irAEs. This review discusses the spectrum of irAEs that can be encountered. Ongoing trials are exploring the use of immunotherapy at earlier stages of uro‐oncological diseases, thus underscoring the evolving landscape of urological cancer treatment. Paradoxically, some data suggests that the occurrence of irAEs is associated with improved oncological outcomes.ConclusionsImmune‐related AEs, while manageable, may be life‐threatening and require lifelong therapy. A thorough understanding of AEs and toxicity of a novel drug class is imperative.