bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surgical management of ameloblastomas: conservative or radical approach? A critical review of the literature
Authors: Adeyemo W.L. , Bamgbose B.O. , Ladeinde A.L. , Ogunlewe M.O. 
Published: February 2008

AbstractAim:  The aim of this study is to review the pertinent literature and determine the most appropriate method of treatment for ameloblastomas.Methods:  A computerized literature search using MEDLINE was conducted for published articles on treatment of ameloblastomas. Mesh phrases used in the search were: ameloblastoma AND treatment; ameloblastoma AND surgical management. An attempt was made to conduct a systematic review on the subject, but due to inconsistency in terminology, treatment protocol, lack of randomised controlled trial and inadequate follow‐up assessment in most of the articles assessed, a narrative critical review of selected relevant literature regarding treatment of ameloblastoma was undertaken.Results:  It is widely reported that the recurrence of an ameloblastoma in large part reflects the inadequacy or failure of the primary surgical procedure. Recent studies have unequivocally showed that when a diagnosis of ameloblastoma is made, the treatment must be aggressive and radical to avoid recurrence. The recurrence rates of 55% to 90% for solid or multicystic lesions treated by conservative approach (enucleation or curettage) and even metastases have been reported. Regarding unicystic ameloblastoma, systematic review of the literature has shown that radical approach to treatment resulted in lowest recurrence rate. For ameloblastomas, the first operation (especially radical) affords the best chance for cure.Conclusions:  There is a lack of consensus on the most appropriate treatment modality for ameloblastomas. However, a more radical approach (whenever possible) appears to be the best method for the management of these benign, but locally aggressive, lesions with propensity for multiple recurrences.
Strawberry gums in Wegener's granulomatosis: a rare presentation
Authors: Paul R. , Moran N. 
Published: February 2008

AbstractWegener's granulomatosis is a multisystem disease characterised by necrotising granulomatosis of the upper and lower respiratory tracts, disseminated vasculitis and glomerulonephritis. However, the clinical manifestations and organs involved in the disease vary widely. This case report documents the importance of considering Wegener's granulomatosis in patients with unique oral lesions. Failure to recognise clinical lesions can result in delayed diagnosis and treatment, with potentially fatal results.
Facteurs epsilon p-adiques
Authors: Marmora Adriano 
Published: February 2008

AbstractWe develop and study the epsilon factor of a ‘local system’ of p-adic coefficients over the spectrum of a complete discrete valuation field K with finite residue field of characteristic p>0. In the equal characteristic case, we define the epsilon factor of an overconvergent F-isocrystal over Spec(K), using the p-adic monodromy theorem. We conjecture a global formula, the p-adic product formula, analogous to Deligne’s formula for étale ℓ-adic sheaves proved by Laumon, which explains the importance of this local invariant. Namely, for an overconvergent F-isocrystal over an open subset of a projective smooth curve X, the constant of the functional equation of the L-series is expressed as a product of the local epsilon factors at the points of X. We prove the conjecture for rank-one overconvergent F-isocrystals and for finite unit-root overconvergent F-isocrystals. In the mixed characteristic case, we study the behavior of the epsilon factor by deformation to the field of norms.
Modulation of β-Catenin and E-Cadherin Interaction by Vpu Increases Human Immunodeficiency Virus Type 1 Particle Release
Authors: Salim Aneeza , Ratner Lee 
Published: February 2008

ABSTRACTVpu (viral protein U) is a 17-kDa human immunodeficiency virus type 1 (HIV-1) accessory protein that enhances the release of particles from the surfaces of infected cells. Vpu recruits β-transducin repeat-containing protein (β-TrCP) and mediates proteasomal degradation of CD4. By sequestering β-TrCP away from other cellular substrates, Vpu leads to the stabilization of β-TrCP substrates such as β-catenin, IκBα, ATF4, and Cdc25A, but not of other substrates such as Emi1. This study shows that in addition to stabilizing β-catenin, Vpu leads to the depression of both total and β-catenin-associated E-cadherin levels through β-TrCP-dependent stabilization of the transcriptional repressor Snail. We showed that both downregulation of overall E-cadherin levels and dissociation of E-cadherin from β-catenin result in enhanced viral release. By contrast, the overexpression of E-cadherin or the prevention of the dissociation of E-cadherin from β-catenin results in depressed levels of virus release. Since E-cadherin is expressed only in dendritic cells and macrophages, and not in T cells, our data suggest that the HIV-1vpugene may have evolved to counteract different restrictions to assembly in different cells.
Highly Enhanced Expression of CD70 on Human T-Lymphotropic Virus Type 1-Carrying T-Cell Lines and Adult T-Cell Leukemia Cells
Authors: Baba Masanori , Okamoto Mika , Hamasaki Takayuki , Horai Sawako , Wang Xin , Ito Yuji , Suda Yasuo , Arima Naomichi 
Published: February 2008

ABSTRACTHuman T-lymphotropic virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL). In Japan, the number of HTLV-1 carriers is estimated to be 1.2 million and more than 700 cases of ATL have been diagnosed every year. Considering the poor prognosis and lack of curative therapy of ATL, it seems mandatory to establish an effective strategy for the treatment of ATL. In this study, we attempted to identify the cell surface molecules that will become suitable targets of antibodies for anti-ATL therapy. The expression levels of approximately 40,000 host genes of three human T-cell lines carrying HTLV-1 genomes were analyzed by oligonucleotide microarray and compared with the expression levels of the genes in an HTLV-1-negative T-cell line. The HTLV-1-carrying T-cell lines used for experiments had totally different expression patterns of viral genome. Among the genes evaluated, the expression levels of 108 genes were found to be enhanced more than 10-fold in all of the T-cell lines examined and 11 of the 108 genes were considered to generate the proteins expressed on the cell surface. In particular, the CD70 gene was upregulated more than 1,000-fold and the enhanced expression of the CD70 molecule was confirmed by laser flow cytometry for various HTLV-1-carrying T-cell lines and primary CD4+T cells isolated from acute-type ATL patients. Such expression was not observed for primary CD4+T cells isolated from healthy donors. Since CD70 expression is strictly restricted in normal tissues, such as highly activated T and B cells, CD70 appears to be a potential target for effective antibody therapy against ATL.
hAda3 Degradation by Papillomavirus Type 16 E6 Correlates with Abrogation of the p14ARF-p53 Pathway and Efficient Immortalization of Human Mammary Epithelial Cells
Authors: Shamanin Vladimir A. , Sekaric Pedja , Androphy Elliot J. 
Published: February 2008

ABSTRACT
Two activities of human papillomavirus type 16 E6 (HPV16 E6) are proposed to contribute to the efficient immortalization of human epithelial cells: the degradation of p53 protein and the induction of telomerase. However, the requirement for p53 inactivation has been debated. Another E6 target is the hAda3 protein, a p53 coactivator and a component of histone acetyltransferase complexes. We have previously described the role of hAda3 and p53 acetylation in p14ARF-induced human mammary epithelial cell (MEC) senescence (P. Sekaric, V. A. Shamanin, J. Luo, and E. J. Androphy, Oncogene 26:6261-6268, 2007). In this study, we analyzed a set of HPV16 E6 mutants for the ability to induce hAda3 degradation. E6 mutants that degrade hAda3 but not p53 could abrogate p14ARF-induced growth arrest despite the presence of normal levels of p53 and efficiently immortalized MECs. However, two E6 mutants that previously were reported to immortalize MECs with low efficiency were found to be defective for both p53 and hAda3 degradation. We found that these immortal MECs select for reduced p53 protein levels through a proteasome-dependent mechanism. The findings strongly imply that the inactivation of the p14ARF-p53 pathway, either by the E6-mediated degradation of p53 or hAda3 or by cellular adaptation, is required for MEC immortalization.
Role for MyD88 Signaling in Murine Gammaherpesvirus 68 Latency
Authors: Gargano Lisa M. , Moser Janice M. , Speck Samuel H. 
Published: February 2008

ABSTRACT

Toll-like receptors (TLRs) are known predominantly for their role in activating the innate immune response. Recently, TLR signaling via MyD88 has been reported to play an important function in development of a B-cell response. Since B cells are a major latency reservoir for murine gammaherpesvirus 68 (MHV68), we investigated the role of TLR signaling in the establishment and maintenance of MHV68 latency in vivo. Mice deficient in MyD88 (MyD88
−/−
) or TLR3 (TLR3
−/−
) were infected with MHV68. Analysis of splenocytes recovered at day 16 postinfection from MyD88
−/−
mice compared to those from wild-type control mice revealed a lower frequency of (i) activated B cells, (ii) germinal-center B cells, and (iii) class-switched B cells. Accompanying this substantial defect in the B-cell response was an approximately 10-fold decrease in the establishment of splenic latency. In contrast, no defect in viral latency was observed in TLR3
−/−
mice. Analysis of MHV68-specific antibody responses also demonstrated a substantial decrease in the kinetics of the response in MyD88
−/−
mice. Analysis of wild-type × MyD88
−/−
mixed-bone-marrow chimeric mice demonstrated that there is a selective failure of MyD88
−/−
B cells to participate in germinal-center reactions as well as to become activated and undergo class switching. In addition, while MHV68 established latency efficiently in the MyD88-sufficient B cells, there was again a ca. 10-fold reduction in the frequency of MyD88
−/−
B cells harboring latent MHV68. This phenotype indicates that MyD88 is important for the establishment of MHV68 latency and is directly related to the role of MyD88 in the generation of a B-cell response. Furthermore, the generation of a B-cell response to MHV68 was intrinsic to B cells and was independent of the interleukin-1 receptor, a cytokine receptor that also signals through MyD88. These data provide evidence for a unique role for MyD88 in the establishment of MHV68 latency.
Human CAR Gene Expression in Nonpermissive Hamster Cells Boosts Entry of Type 12 Adenovirions and Nuclear Import of Viral DNA
Authors: Hochstein Norbert , Webb Dennis , Hösel Marianna , Seidel Werner , Auerochs Sabrina , Doerfler Walter 
Published: February 2008

ABSTRACT

Adenovirus type 12 (Ad12) propagation in hamster BHK21 cells is blocked prior to viral DNA replication. The amounts of Ad12 DNA in the nuclei or cytoplasm of hamster cells are about 2 orders of magnitude (2 h postinfection [p.i.]) and 4 to 5 orders of magnitude (48 h p.i.) lower than in permissive human cells. Cell line BHK21-hCAR is transgenic for and expresses the human coxsackie- and adenovirus receptor (hCAR) gene. Nuclear uptake of Ad12 DNA in BHK21-hCAR cells is markedly increased compared to that in naïve BHK21 cells. Ad12 elicits a cytopathic effect in BHK21-hCAR cells but not in BHK21 cells. Quantitative PCR or [
3
H]thymidine labeling followed by zone velocity sedimentation fails to detect Ad12 DNA replication in BHK21 or BHK21-hCAR cells. Newly assembled Ad12 virions cannot be detected. Thus, the block in Ad12 DNA replication in hamster cells is not released by enhanced nuclear import of Ad12 DNA.
Ownership and uses of human tissue: what are the opinions of surgical in-patients?
Authors: Bryant R J , Harrison R F , Start R D , Chetwood A S A , Chesshire A M , Reed M W R , Cross S S 
Published: February 2008

Aims:To investigate whether patient opinion about the uses of tissue removed at therapeutic operations has changed since the adverse publicity surrounding the Alder Hey and Bristol Royal Infirmary Inquiries, and to see whether it aligns with the Human Tissue Act 2004.Methods:A questionnaire was given to 220 postoperative patients in a teaching hospital during an 11 week period. Aggregated responses to each question were ranked in frequency order. Unweighted centroid linkage hierarchical clustering analysis was performed with dendrogram display for the main data on tissue usage.Results:203 completed questionnaires were collected (compliance rate 92.3%). 96.3% of patients indicated that they would not object to their tissue being used in research, significantly higher than in the 1996 study (89.1%) with no overlap of the 95% CIs. 29.1% of patients believed that the hospital had ownership of tissue once it has been removed during surgery, 23.2% believed they had ownership, 19.7% believed that the pathology laboratory had ownership, and 15.3% believed that nobody had ownership rights in the case of tissue samples.Conclusions:This new survey indicates that despite a turbulent decade for those involved in human tissue retention in the UK, public support for a wide range of human tissue based activities, especially biomedical research, has not diminished and that patient opinion aligns well with the Human Tissue Act 2004.
Subseasonal Variability Associated with Asian Summer Monsoon Simulated by 14 IPCC AR4 Coupled GCMs
Authors: Lin Jia-Lin , Weickman Klaus M. , Kiladis George N. , Mapes Brian E. , Schubert Siegfried D. , Suarez Max J. , Bacmeister Julio T. , Lee Myong-In 
Published: February 2008

Abstract
This study evaluates the subseasonal variability associated with the Asian summer monsoon in 14 coupled general circulation models (GCMs) participating in the Intergovernmental Panel on Climate Change (IPCC) Fourth Assessment Report (AR4). Eight years of each model’s twentieth-century climate simulation are analyzed. The authors focus on the three major components of Asian summer monsoon: the Indian summer monsoon (ISM), the western North Pacific summer monsoon (WNPSM), and the East Asian summer monsoon (EASM), together with the two dominant subseasonal modes: the eastward- and northward-propagating boreal summer intraseasonal oscillation (BSIO) and the westward-propagating 12–24-day mode.
The results show that current state-of-the-art GCMs still have difficulties and display a wide range of skill in simulating the subseasonal variability associated with Asian summer monsoon. During boreal summer (May–October), most of the models produce reasonable seasonal-mean precipitation over the ISM region, but excessive precipitation over the WNPSM region and insufficient precipitation over the EASM region. In other words, models concentrate their rain too close to the equator in the western Pacific. Most of the models simulate overly weak total subseasonal (2–128 day) variance, as well as too little variance for BSIO and the 12–24-day mode. Only 4–5 models produce spectral peaks in the BSIO and 12–24-day frequency bands; instead, most of the models display too red a spectrum, that is, an overly strong persistence of precipitation. For the seven models with three-dimensional data available, five reproduce the preconditioning of moisture in BSIO but often with a too late starting time, and only three simulate the phase lead of low-level convergence. Interestingly, although models often have difficulty in simulating the eastward propagation of BSIO, they tend to simulate well the northward propagation of BSIO, together with the westward propagation of the 12–24-day mode. The northward propagation in these models is thus not simply a NW–SE-tilted tail protruding off of an eastward-moving deep-tropical intraseasonal oscillation.
Itch−/−αβ and γδ T cells independently contribute to autoimmunity in Itchy mice
Authors: Parravicini Valentino , Field Anne-Christine , Tomlinson Peter D. , Albert Basson M. , Zamoyska Rose 
Published: February 2008

AbstractE3 ubiquitin ligases determine which intracellular proteins are targets of the ubiquitin conjugation pathway and thus play a key role in determining the half-life, subcellular localization and/or activation status of their target proteins. Itchy mice lack the E3 ligase, Itch, and show dysregulation of T lymphocytes and the induction of a lethal autoimmune inflammatory condition. Itch is widely expressed in hematopoietic and nonhematopoietic cells, and we demonstrate that disease is transferred exclusively by hematopoietic cells. Moreover, distinct manifestations of the autoimmune inflammatory phenotype are contributed by discrete populations of lymphocytes. The presence of Itch-deficient αβ T cells drives expansion of peritoneal B1b cells and elevated IgM levels, which correlate with itching and pathology. In contrast, Itch−/− interleukin-4–producing γδ T cells, even in the absence of αβ T cells, are associated with elevated levels of IgE and an inflammatory condition. These data indicate that disruption of an E3 ubiquitin ligase in αβ T cells can subvert a B-cell subpopulation, which normally functions to control particular microbial pathogens in a T-independent manner, to contribute to autoimmunity. In addition, disruption of Itch in innate γδ T cells can influence autoimmune pathology and might therefore require distinct therapeutic intervention.
Imatinib pharmacokinetics and its correlation with response and safety in chronic-phase chronic myeloid leukemia: a subanalysis of the IRIS study
Authors: Larson Richard A. , Druker Brian J. , Guilhot Francois , O'Brien Stephen G. , Riviere Gilles J. , Krahnke Tillmann , Gathmann Insa , Wang Yanfeng 
Published: February 2008

AbstractImatinib at 400 mg daily is standard treatment for chronic myeloid leukemia in chronic phase. We here describe the correlation of imatinib trough plasma concentrations (Cmins) with clinical responses, event-free survival (EFS), and adverse events (AEs). Trough level plasma samples were obtained on day 29 (steady state, n = 351). Plasma concentrations of imatinib and its metabolite CGP74588 were determined by liquid chromatography/mass spectrometry. The overall mean (± SD, CV%) steady-state Cmin for imatinib and CGP74588 were 979 ng/mL (± 530 ng/mL, 54.1%) and 242 ng/mL (± 106 ng/mL, 43.6%), respectively. Cumulative estimated complete cytogenetic response (CCyR) and major molecular response (MMR) rates differed among the quartiles of imatinib trough levels (P = .01 for CCyR, P = .02 for MMR). Cmin of imatinib was significantly higher in patients who achieved CCyR (1009 ± 544 ng/mL vs 812 ± 409 ng/mL, P = .01). Patients with high imatinib exposure had better rates of CCyR and MMR and EFS. An exploratory analysis demonstrated that imatinib trough levels were predictive of higher CCyR independently of Sokal risk group. AE rates were similar among the imatinib quartile categories except fluid retention, rash, myalgia, and anemia, which were more common at higher imatinib concentrations. These results suggest that an adequate plasma concentration of imatinib is important for a good clinical response. This study is registered at http://clinicaltrials.gov as NCT00333840.
Impact of light on Hypocrea jecorina and the multiple cellular roles of ENVOY in this process
Authors: Schuster Andrè , Kubicek Christian P , Friedl Martina A , Druzhinina Irina S , Schmoll Monika 
Published: February 2008

Abstract

Background
In fungi, light is primarily known to influence general morphogenesis and both sexual and asexual sporulation. In order to expand the knowledge on the effect of light in fungi and to determine the role of the light regulatory protein ENVOY in the implementation of this effect, we performed a global screen for genes, which are specifically effected by light in the fungus Hypocrea jecorina (anamorph Trichoderma reesei) using Rapid Subtraction Hybridization (RaSH). Based on these data, we analyzed whether these genes are influenced by ENVOY and if overexpression of ENVOY in darkness would be sufficient to execute its function.

Results
The cellular functions of the detected light responsive genes comprised a variety of roles in transcription, translation, signal transduction, metabolism, and transport. Their response to light with respect to the involvement of ENVOY could be classified as follows: (i) ENVOY-mediated upregulation by light; (ii) ENVOY-independent upregulation by light; (iii) ENVOY-antagonized upregulation by light; ENVOY-dependent repression by light; (iv) ENVOY-independent repression by light; and (v) both positive and negative regulation by ENVOY of genes not responsive to light in the wild-type. ENVOY was found to be crucial for normal growth in light on various carbon sources and is not able to execute its regulatory function if overexpressed in the darkness.

Conclusion
The different responses indicate that light impacts fungi like H. jecorina at several cellular processes, and that it has both positive and negative effects. The data also emphasize that ENVOY has an apparently more widespread cellular role in this process than only in modulating the response to light.
CT scanning for diagnosing blunt ureteral and ureteropelvic junction injuries
Authors: Ortega Sarah J , Netto Fernado S , Hamilton Paul , Chu Peter , Tien Homer C 
Published: February 2008

Abstract

Background
Blunt ureteral and ureteropelvic (UPJ) injuries are extremely rare and very difficult to diagnose. Many of these injuries are missed by the initial trauma evaluation.

Methods
Trauma registry data was used to identify all blunt trauma patients with ureteral or UPJ injuries, from 1 April 2001 to 30 November 2006. Demographics, injury information and outcomes were determined. Chart review was then performed to record initial clinical and all CT findings.

Results
Eight patients had ureteral or UPJ injuries. Subtle findings such as perinephric stranding and hematomas, and low density retroperitoneal fluid were evident on all initial scans, and prompted delayed excretory scans in 7/8 cases. As a result, ureteral and UPJ injuries were diagnosed immediately for these seven patients. These findings were initially missed in the eighth patient because significant associated visceral findings mandated emergency laparotomy. All ureteral and UPJ injuries have completely healed except for the case with the delay in diagnosis.

Conclusion
Most blunt ureteral and UPJ injuries can be identified if delayed excretory CT scans are performed based on initial CT findings of perinephric stranding and hematomas, or the finding of low density retroperitoneal fluid.