bluuebunny/crossref_metadata_2025_split huggingface dataset:
Versatile, accessible cross-platform molecular profiling of central nervous system tumors: web-based, prospective multi-center validation
Authors: Sahm Felix , Patel Areeba , Göbel Kirsten , Hinz Felix , Ille Sebastian , Dogan Helin , Lomakin Artem , Schrimpf Daniel , Göller Pauline , Ritter Michael , Kerbs Paul , Pfeifer Lisa , Schoebe Natalie , Hamelmann Stefan , Blume Christina , Bogumil Henri , Berghaus Natalie , Euskirchen Philipp , Schweizer Leonille , Hultschig Claus , Roy Nadine Van , Dorpe Jo Van , Meulen Joni Van der , Loontiens Siebe , Dedeurwaerdere Franceska , Leske Henning , Halldorsson Skarphedinn , Fox Graeme , Deacon Simon , Cahyani Inswasti , Holmes Nadine , Wibowo Satrio , Munro Rory , Martin Dan , Sharif Abid , Housley Mark , Goldspring Robert , Brandner Sebastian , Roy Somak , Hench Jürgen , Frank Stephan , Unterberg Andreas , Goidts Violaine , Jäger Natalie , Paine Simon , Smith Stuart , Herold-Mende Christel , Wick Wolfgang , Pfister Stefan , Krieg Sandro , Vik-Mo Einar , von Deimling Andreas , Jones David , Loose Matthew , Schlesner Matthias , Sill Martin 
Published: April 2024

Abstract
The 2021 WHO classification underscores the importance of molecular data integration in Central Nervous System (CNS) tumor diagnostics. However, currently used assays have disadvantages due to technical complexity, required equipment and reagent cost, as well as lengthy turnaround times. In response to these challenges, we introduce Rapid-CNS2 and MNP-Flex. Rapid-CNS2, an adaptive sampling-based nanopore sequencing workflow, offers real-time methylation classification and DNA copy-number information within a 30-minute window, suitable for intra-operative settings, followed by comprehensive molecular profiling within 24 h, covering the complete spectrum of diagnostically and therapeutically relevant information for the respective entity. We have prospectively validated Rapid-CNS2 in a multi-center setting on 223 samples. For even more widespread use of methylation-based CNS tumor classification, we developed MNP-Flex, a platform-agnostic methylation classifier encompassing 184 CNS tumor classes. MNP-flex achieved 92% accuracy across a global validation cohort of 78,000 samples spanning five different technologies. These innovations represent a significant advancement in CNS tumor diagnostics, making rapid, actionable molecular insights more widely and more rapidly available, which is crucial for personalized treatment strategies. Their integration streamlines the diagnostic process, broadening access to accurate molecular classification and promising improved patient outcomes in neurooncology on a global scale. MNP-Flex is available as a web-service https://mnp-flex.org and the Rapid-CNS2 workflow is available on Github.
Decisional procrastination and problematic mobile phone use: the mediation of anxiety and the moderation of 2019-nCoV traumatic experience
Authors: Chen Lili , Gao Sun-Yu , Li Jun , Chen Ling-Ge , Huang Jian-Hao 
Published: April 2024

Abstract

Background Decisional procrastination (DP) has an important effect on problematic mobile phone use (PMPU); however, the potential mechanisism and boundary conditions between DP and PMPU remianed to be further explored. This essay studied the mediation of anxiety between DP and PMPU and whether 2019-nCoV traumatic experience moderated the mediation process.Methods A questionnaire was used in this study. A valid sample of 798 college students reported levels of decisional procrastination, problematic mobile phone use, anxiety, and 2019-nCoV traumatic experience.Results The results indicated that decisional procrastination is positively associated with problematic mobile phone use among college students. Anxiety served as a partial mediator in the association between decisional procrastination and problematic mobile phone use. 2019-nCoV traumatic experience would positively moderated the mediating effects of anxiety between DP and PMPU. A higher degree of 2019-nCoV traumatic experience would strengthen the mediation effects of DP to PMPU through anxiety.Conclusions This study deepens our understanding of how DP affects college students' PMPU. It was found in the study that 2019-nCoV traumatic experience positively moderated the mediation effects of anxiety between DP and PMPU. These findings provide universities with a theoretical foundation for preventing PMPU among college students.
Genetic diversity and population structure analysis of Ethiopian bread wheat (Triticum eastivum L.) germplasm using SSR markers
Authors: Tiruneh Abebe , Feyissa Tileye 
Published: April 2024

Abstract

Background: Bread wheat, belonging to the most diverse and important family Poaceae in the plant kingdom, produces crucial edible grains. Ethiopia has been considered as a center of diversity and the second center of bread wheat domestication. Genetic diversity and population structure analyses in the Ethiopian bread wheat germplasm have enormous importance in enhancing breeding and sustainable conservation.
Methodology: 96 bread wheat germplasm were gathered from Kulumsa and Adet Research Centers, Ethiopia. The samples were taken to the ICARDA-BIGMP, Cairo, Egypt and grown at the green house, after two weeks leaf samples were collected per plant, and taken to the laboratory for DNA extraction. Data were analyzed using PowerMarker ver. 3.25, NJ, UPGMA, Structure, ver.2.3.4, and AMOVA.
Results: Genetic diversity and population structure were estimated across 96 germplasm using 7 polymorphic and informative SSRs. Varied values of diversity indices were observed across chromosomes and genomes. Higher mean values of MAF (0.67), PIC (0.34), and Nei's gene diversity (Gd) (0.36), and values of Gd (0.41) and PPL (87.28%) were signifying the presence of high genetic diversity within and among populations, respectively. AMOVA showed highly significant population differentiation for 98% variation within population letting only 2% significant variation among populations. The Structure analysis showed four populations (COV, EBWNVT, EBWYT, and EBWAT), while the UPGMA revealed 3 main population clusters, in which the EBWYT and EBWAT were the 2 sub-clusters. The NJ analysis and PCoA across 96 germplasm revealed three main clusters in each the germplasm were found inter-mixed irrespective of their breeding history and evolution likely to the Clumpak result, signifying the presence of higher admixture due to the existence of historical exchanges of seeds through informal system involving regional and nationwide farming communities in Ethiopia.
Conclusions: Sustainable utilization and conservation of rich Ethiopian bread wheat genetic resource is an irreplaceable means to cope up the recurrent climate changes and biotic stresses happening wide, and thereby able to meet the demand of bread wheat productivity for the ever-growing human population.
Proteomic Analysis of Plasma at the Preterminal Stage of Rhesus Nonhuman Primates Exposed to a Lethal Total-Body Dose of Gamma-Radiation
Authors: Carpenter Alana D. , Fatanmi Oluseyi O. , Wise Stephen Y. , Tyburski John B. , Cheema Amrita K. , Singh Vijay K. 
Published: April 2024

Abstract

The identification and validation of radiation biomarkers is critical for assessing the radiation dose received in exposed individuals and for developing radiation medical countermeasures that can be used to treat acute radiation syndrome (ARS). Additionally, a fundamental understanding of the effects of radiation injury could further aid in the identification and development of therapeutic targets for mitigating radiation damage. In this study, blood samples were collected from fourteen male nonhuman primates (NHPs) that were exposed to 7.2 Gy ionizing radiation at various time points (seven days prior to irradiation; 1, 13, and 25 days post-irradiation; as well as immediately prior to the euthanasia of moribund animals (preterminal)). Plasma was isolated from these samples and was analyzed using a liquid chromatography tandem mass spectrometry approach in an effort to determine the effects of radiation on plasma proteomic profiles. Of particular interest was to determine if the expression of certain proteins reacted to radiation in a way that would act as a predictor for health decline leading to a preterminal phenotype. Our results suggest that radiation induced a diverse temporal pattern among protein expression that displayed prominent changes within NHP proteomic plasma profiles. Of these significantly altered proteins, several play important roles in certain biological processes such as hemostasis, inflammation, and immune response.
Roles of Ce1-xZrxO2/SBA-15 in selective catalytic glycerol conversion to lactic acid process: Process behaviors and kinetic analyses
Authors: Saleh Syamima Nasrin Mohamed , Rohman Fakhrony Solahuddin Sholahudin , Ul-Hamid Anwar , ABDULLAH AHMAD ZUHAIRI BIN 
Published: April 2024

Abstract

The surge in glycerol production from oleochemical industry prompted the aggressive search for new value-added derivatives such as lactic acid. A series of novel Ce1 − xZrxO2 catalysts on mesoporous SBA-15 were synthesized and tested in a 50 mL reactor for up to 2 hours for oxidative lactic acid synthesis from glycerol. A suitable kinetic model to represent the reaction was identified and kinetic parameters were calculated. The 10Ce1 − xZrxO2/SBA-15 catalyst, at a 15 wt.% catalyst-to-glycerol ratio, exhibited impressive activity, achieving 94.2% glycerol conversion and 45.3% lactic acid yield at 260°C. The reaction model shows good agreement with experimental data across various reaction conditions (R2 values between 0.90 and 1.00). Based on a pseudo-second-order kinetic model, the selective conversion of glycerol to lactic acid was found to have an activation energy of 165.3 kJ/mol, with a high R2 value of 0.94 which was lower than some reported results.
The Role of Green Finance in Promoting Sustainable Development Goals (SDGs) Through the Mediation Effect of Green Technology Innovation
Authors: Mahmood Shahid , Sun Huaping , Iqbal Asifa , Alharbi Amal H. , Khafaga Doaa Sami , El-kenawy El-Sayed M. 
Published: April 2024

Abstract

Nations are confronted with a significant challenge in attaining the Sustainable Development Goals (SDGs). Achieving the SDGs pertaining to social sustainability, economic sustainability, and environmental sustainability poses the greatest challenge due to various interconnected factors. The present study investigates the influence of green finance on sustainable infrastructure, innovation in green technology, corporate social responsibility, economic stability, and environmental conservation within the framework of Belt and Road initiative (BRI) funding, particularly focusing on its application in the China-Pakistan Economic Corridor (CPEC) initiatives. A questionnaire served as the primary instrument for data collection. The study gathered data from a diverse sample comprising businessmen, CPEC officials, representatives from the Ministry of Finance, Pakistan Environmental Protection Agency, Ministry of Planning and Development. In this study, the PLS 4 was employed to examine associations and test hypotheses. The findings suggest a significant impact of green finance on both sustainable infrastructure and green technology innovation. Furthermore, the results underscore the vital role of environmentally friendly technology and sustainable infrastructure in advancing the SDGs across social, economic, and environmental dimensions. The study's results can help policymakers in other countries collaborate on similar projects to boost economic growth and environmental sustainability. This involves integrating green technologies, sustainable infrastructure, and green finance practices.
Role of vascularised fibula graft in the surgical management of Radiation-Induced Midshaft Femoral Fractures. Case Report and Literature Review.
Authors: Khasin Monique , Darcy Genevieve , Mah Eldon , Bella A/Prof Claudia Di 
Published: April 2024

Abstract

Background:
Post-radiation fractures (PRF) are a recognised complication of radiation treatment for soft tissue sarcomas. They have a low incidence and typically occur up to 5 years following treatment, more commonly affecting the pelvis, ribs and femur. Due to radiation-induced changes in bone, PRFs typically require more complicated intervention compared to post-trauma fractures, however, limited literature exists, particularly in regards to mid-shaft femoral PRFs. We report a case of a mid-shaft femoral PRF managed with onlay free vascularised fibular grafting (FVFG) due to non-union following initial intramedullary fixation.
Case Presentation:
A 40-year-old male with a history of left quadriceps clear cell sarcoma successfully treated with wide local excision, chemotherapy and radiotherapy 18 years prior presented with a displaced oblique pathological fracture of his left femoral shaft. He was initially treated operatively with intramedullary (IM) nailing, however, repeat imaging at the one-year post-operative review demonstrated persistent hypotrophic non-union of the fracture. 16 months following the initial fracture, the patient underwent further surgical intervention with implantation of a FVFG to the anterior aspect of the distal femur using bone from the right fibula and applied onlay without nail removal. One-year post-revision, the patient was pain-free with normal mobility and imaging of both the graft and fracture site demonstrated complete union.
Conclusion:
Despite their operative complexity, we suggest that FVFGs should be considered for treating non-union of mid-shaft femoral PRFs due to their ability to promote healing and bone union in irradiated bone. They could also be considered as a first line treatment for patients who are at high risk of non-union with conventional fracture fixation methods.
Analysis of gastric electrical rhythm in patients with type 2 diabetes mellitus
Authors: Wang Xixi , Ma Lianhua , Jiang Miao , Zhu Hong , Ni Chendong , Yang Xiaohua , Hu Ji , Zhang Hong-Hong 
Published: April 2024

Abstract

Aim: To analysis the change of electrogastrogram (EGG) in patients with type 2 diabetes mellitus (T2DM), and evaluate the prevalence of abnormal gastric electrical rhythm (AGER) and its relative influencing factors.
Methods: A total of 65 patients with T2DM hospitalized at the Second Affiliated Hospital of Soochow University from Dec.2020 to Dec. 2021 were included in the cross-sectional study. General information, clinical data, and medical history data of all study subjects, including name, gender, body mass index (BMI), duration of diabetes, anti-diabetic therapies, high blood pressure (HBP) history, smoking history and medication history, were completely collected. The results of laboratory tests, including biochemical parameters, glycosylated hemoglobin (HbA1c), fasting C-peptide, 2h postprandial C-peptide, 24-hour urine total protein (24-hUTP), urine microalbumin creatinine ratio (UACR) and estimated glomerular filtration rate (eGFR) were recorded. EGG, Gastroparesis Cardinal Symptom Index (GCSI), gastric emptying ultrasound, fundus examination, carotid artery ultrasonography, cardiac autonomic function test, heart rate variability (HRV) were all examined and recorded as well. According to the results of EGG, the subjects were divided into normal gastric electrical rhythm (NGER) group and abnormal gastric electrical rhythm (AGER) group.
Results: (1) Fasting blood glucose (FBG), HbA1c, the presence of diabetic peripheral neuropathy (DPN) and diabetic cardiac autonomic neuropathy (DCAN) were significantly higher in the AGER group (p<0.05). Low frequency (LF) and high frequency (HF), the indicators of HRV, were significantly lower in the AGER group (p<0.05). In addition, the prevalence of feeling excessively full after meals, loss of appetite and stomach or belly visibly larger after meals of gastrointestinal symptoms of gastroparesis were significantly higher in the AGER group (p<0.05). Multiple logistic regression analysis showed that FBG and the prevalence of DCAN were the independent risk factors.
Conclusion: AGER was associated with high FBG and the presence of DCAN. EGG examination is recommended for patients with gastrointestinal symptoms and clues of DCAN.
Effect of post-weld heat treatment on AA6351 friction stir welds
Authors: MADDA RAGHAVA RAO , B Lakshmi Saranya , G Siva Prasad , Ch Venkata Rao , K Srinivasa Rao 
Published: April 2024

Abstract

Al-Mg-Si (6xxx) alloys have superior properties to other aluminium alloys, making them suitable for aerospace and defence applications. However, their weldments are susceptible to various issues that affect their microstructural changes, mechanical properties, and corrosion resistance. The use of friction stir welding has been proven to be an effective solution for these problems, and this study aimed to investigate the microstructural changes, mechanical properties, and pitting potential (Epit) of AA6351 alloy friction stir welds. The present study used AA6351 with a thickness of 6mm alloy plates for welding and characterized the welds' microstructure, pitting corrosion resistance, and mechanical properties of hardness and tensile strength. Post-weld heat treatment (PWHT) for solutionizing at 523°C for 0.5hrs followed by 8 hrs at 177⁰C (STA-solutionizing treatment with ageing) was applied to improve the welds' corrosion resistance and mechanical properties. A comparison between the base metal and weldment before and after PWHT showed that the ageing treatment of the welds improved their mechanical properties and pitting corrosion resistance. This treatment caused re-precipitation and redistribution of precipitates in the welds’ grain-refined stir zone, restoring their mechanical properties and pitting corrosion resistance. Therefore, this study established that post-weld solutionizing at 523°C for 0.5 hrs and then ageing at 177⁰C for 8hrs can restore the overall mechanical and pitting corrosion behaviour of AA6351 alloy friction stir welds.
Prevalence and Predictors of Stillbirths Following Vaginal Delivery at Tumu Municipality of the Upper West Region, Ghana: a cross-sectional study
Authors: Alhassan Pascal Deeshini Aliu , Beru Martin Muonibe , Sackeya Eugene , Angmortey Richard Nomo , Alhassan Abdul-Samii Danaa , Kanamu Mohammed Hafiz , Akpah-Suka Andrews , Mohammed Baba Sulemana 
Published: April 2024

Abstract

Background
Low- and middle-income countries still have unacceptably high rates that makes it imperative to identify the factors that predict stillbirth so as to effectively develop interventions. This study aimed to identify the prevalence and predictors of stillbirth among mothers who gave birth in the Tumu municipality in the Upper West Region of Ghana.
Methods
Using a retrospective cross-sectional study design, 1,240 women who had vaginal deliveries had their medical records examined. From January 1 to December 31, 2019, information on vaginal deliveries was gathered from the hospital's records. The data were summarised using descriptive statistics, and the relationship between maternal and obstetric characteristics and foetal outcomes was investigated using Pearson's chi-square/Fisher's exact test. To evaluate predictors, logistic regression was fitted at a 95% confidence interval (95% CI), and the results were reported using odds ratio.
Results
Among the 1204 women, 626 (52%) of them were aged 20–29 years; 832 (70%) of mothers were multigravida and 878 (73.50%) attended ANC between 4 to 8 times. There were 1187 (98.6%) live births and the overall prevalence of foetal mortality was 14.12 per 1000 total births. Women who attended ANC between 4–8 times were 91% less likely to record a foetal mortality (AOR 0.090 (0.024–0.342) p-value = < 0.001). Women whose labour was not monitored using a partograph were more than 17 times likely to record a foetal mortality (AOR 17.156, 95% CL: 4.981–59.090, p-value = < 0.001).
Conclusion
The neonatal mortality rate is 14.12 per 1000 live births, with better outcomes for pregnant women who attend ANC 4–8 times. The likelihood of foetal deaths in labors without partograph monitoring is 17 times higher. Refresher training on patographs and early hospital arrival are recommended.
Dendritic cells pulsed with breast tumor lysate-derived nano-vesicles can elicit an effective in vitro and in vivo anti-tumor immune response
Authors: abbaspour maryam , Esmaeil Nafiseh , Rezazadeh Mahboubeh , Minaiyan Mohsen , Sanei Mohammad hossein , Akbari Vajihe 
Published: April 2024

Abstract
Background

This study aimed to investigate the effectiveness of utilizing autologous whole tumor cell lysate as a therapeutic vaccine to improve antigen presentation and processing to immune cells and induce an appropriate immune response. Nano-vesicles derived from mouse breast cancer cell lysate (TCL-NVs) were obtained through sonication.In vitro, the effect of TCL-NVs on dendritic cell (DC) maturation and T-cell proliferation using flow cytometry. In vivo studies evaluated the antitumor efficacy and safety of DCs loaded with TCL-NVs (DC-TCL-NVs) in a breast tumor mouse model. The levels of cytokines and infiltration of T cells at the tumor site were analyzed through RT-PCR and immunohistochemistry.

Results

The results showed that DCs loaded with TCL-NVs displayed a significant increase in CD86 expression compared to the control group (DCs pulsed only with LPS) (*P < 0.02). Furthermore, the TCL-NVs-loaded DCs exhibited an upregulation of genes involved in antigen presentation and T-cell priming compared to the control group (P<0.005). These activated DCs promoted T-cell proliferation and produced high levels of IFN-γ but low levels of IL-10, indicating their potential to elicit a TH1-immune response. Furthermore, administration of TCL-NV-loaded DCs in Balb/c mice induced a strong antitumor response in vivo, which reduced tumor growth compared to the control group (P<0.0001). Additionally, DC-TCL-NVs showed higher levels of tumor infiltration by CD8+ cells and increased gene expression of pro-inflammatory cytokines when compared to the control group.

Conclusions

These results underscore the immune-boosting potential of DC vaccines loaded with nano-vesicles obtained from autologous tumor cell lysate.