bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colchicine alleviates interstitial lung disease in mice with experimental autoimmune myositis by inhibiting the formation of neutrophil extracellular traps
Authors: Li Feifei , Zhao Peipei , Zhao Liangtao , Bai Ling , Su Qiyan , Feng Yingyue , Ma Wenlan , Zhu Jiarui , Zhang Sigong 
Published: March 2024

Abstract

Background:Our previous study has shown that neutrophil extracellular traps (NETs) are associated with idiopathic inflammatory myopathy and its related interstitial lung disease (IIM-ILD). Colchicine plays an anti-inflammatory role mainly by inhibiting the activity and chemotaxis of neutrophils. This study will verify the role and mechanism of colchicine in IIM-ILD.
Methods: A mouse model of experimental autoimmune myositis was established and divided into model group, colchicine treatment group (1, 2mg/kg), and healthy control group (n=5/ group). After 5 weeks, the mice were sacrificed to evaluate the degree of pulmonary interstitial lesions and the formation of NETs. Human neutrophils were stimulated with PMA in vitro, and the treatment group was pretreated with colchicine (40nmol/L). After 4 hours of culture, the neutrophils were stained with Sytox Green and observed by fluorescence microscopy. Human pulmonary microvascular endothelial cells were stimulated with NETs, and the experimental group was pretreated with colchicine (7.5, 15nmol/L), and the markers of inflammation and pyroptosis were detected 24h later.
Results: (1) Pathological staining of lung tissue showed that colchicine treatment significantly alleviated interstitial lung disease in EAM mice. (2) After colchicine treatment, the infiltration of NETs in the lung tissue of EAM was significantly reduced, and the serum level of NETs was also significantly decreased (F=6.859, P < 0.05). (3) Colchicine intervention significantly attenuated the formation of NETs on neutrophils induced by PMA in vitro. (4) Colchicine significantly decreased the expression of inflammasomes and pyroptosis markers in NETs-stimulated human lung microvascular endothelial cells, and inhibited the production of inflammasomes and pyroptosis in the lung tissue of EAM in vivo.
Conclusions: Colchicine can alleviate interstitial lung disease in EAM mice by inhibiting NETs formation, inflammasome activation and endothelial cell pyroptosis. These findings provide a basis for targeting NETs in the treatment of myositis-associated interstitial lung disease.
Tuberculosis of TMJ presenting as preauricular swelling – A rare case report
Authors: Lata Jeevan , Gupta Pallavi , Pansotra Neetu , Gowda Sharad 
Published: March 2024

Abstract
Tuberculosis is an infectious disease affecting multiple organs commonly caused by Mycobacterium tuberculosis. It can present as either its primary form or in secondary forms. Primary TB is mostly pulmonary, but it may occur in skin, abdomen, joints, lymph nodes, meninges and central nervous system. Maxillofacial manifestations of tuberculosis form nearly 10% of all extrapulmonary manifestations of the disease. Very few cases of primary TB of the temporomandibular joint (TMJ) have been reported in the literature. This rarity is the reason for not considering TB in differential diagnosis of the patients presenting with TMJ pain. Owing to its non-specific clinical signs it may be misdiagnosed as osteoarthritis, osteomyelitis, or any other chronic joint disease. Detailed clinical and radiographic examination aided by histopathological and a microbiological diagnostic workup is the key for timely detection and administration of appropriate therapeutic regimens.
Investigating the impact of Socio-Demographic and Urban Built Environments on the Spatial Heterogeneity of Flu Vaccine Locations – A Case Study from New York City, United States
Authors: Chang Haoliang , Huang Siwen , Luo Shuli , Han Ahreum , XU Corey Kewei 
Published: March 2024

Abstract
Flu vaccine clinics play a pivotal role in fighting the spread of flu. However, limited research has focused on the influence of socio-demographic and urban built environments on the spatial heterogeneity of flu vaccine locations. This study focuses on the spatial inequity of flu vaccine clinics in New York City. Firstly, Global Moran’s I index was utilized to test the statistical significance of the spatial heterogeneity of flu vaccine locations. Next, ordinary least squares regression, a spatial lagged endogenous model, and two geographically weighted regression models were conducted to uncover the associations between the number of flu vaccine locations and the socio-demographic and urban built environments. We observed significant spatial heterogeneity regarding the spatial distribution of all flu vaccine locations and clinics offering flu vaccines for children. Population distribution and per capita income were positively associated with the number of flu vaccine locations and clinics offering flu vaccines for children. Compared to all the flu vaccine locations associated with regions offering greater health services, the clinics that offered flu vaccines for children were placed in areas with more numerous residential communities. Current findings indicate that socio-demographics and the urban built environment have distinct influences on the number of flu vaccine clinics in different city regions. These findings provide the health authorities with valuable insights into the socio-demographic and urban characteristics associated with the inequity of flu vaccine clinics and may enhance preparedness for future influenza pandemics.
Mycobacterium marinum MMAR_0267-regulated copper utilization facilitates bacterial escape from phagolysosome
Authors: xu Junqi , Ma Shaying , Huang Yu , Zhang Qiao , huang lingxi , xu xianghong , Suleiman Ismail , li peibo , Wang ZhiJian , Xie Jianping 
Published: March 2024

Abstract

The host limits Mycobacterium tuberculosis (Mtb) by restricting copper access. This research investigates how Mtb escapes copper stress. The membrane protein encoded by Mtb Rv0102, when its homolog in M. smegmatis (MSMEG_4702) was knocked out, resulted in a fourfold decrease in intracellular copper levels and enhanced tolerance to elevated extracellular copper concentrations. Similarly, knockout mutants of its homolog in M. marinum (MMAR_0267) showed increased virulence in zebrafish and higher bacterial load within macrophages. In THP-1 cells infected with MMAR_0267 deletion mutants, the intracellular survival of the mutants increased, accompanied by reduced THP-1 apoptosis. Cu deficiency down-regulated the transcriptional level of the M. marinum virulence factor CFP-10, dampened macrophage STING cytosolic signaling, resulting in decreased IFN-β production and cell apoptosis. In conclusion, these findings highlight the significant impact of copper on the survival and reproduction of mycobacteria, underscoring the importance of studying mycobacterial adaptation mechanisms in copper-rich environments.
The putative proton-coupled organic cation antiporter is involved in uptake of triptans into human brain capillary endothelial cells
Authors: Svane Nana , Pedersen Alberte Bay Villekjaer , Rodenberg Anne , Ozgür Burak , Saaby Lasse , Bundgaard Christoffer , Kristensen Mie , Tfelt-Hansen Peer , Brodin Birger 
Published: March 2024

Abstract

Background
Triptans are anti-migraine drugs with a potential central site of action. However, it is not known to what extent triptans cross the blood-brain barrier (BBB). The aim of this study was therefore to determine if triptans pass the brain capillary endothelium and investigate the possible underlying mechanisms with focus on the involvement of the putative proton-coupled organic cation (H+/OC) antiporter. Additionally, we evaluated whether triptans interacted with the efflux transporter, P-glycoprotein (P-gp).
Methods
We investigated the cellular uptake characteristics of the prototypical H+/OC antiporter substrates, pyrilamine and oxycodone, and seven different triptans in the human brain microvascular endothelial cell line, hCMEC/D3. Triptan interactions with P-gp were studied using the IPEC-J2 MDR1 cell line. Lastly, in vivo neuropharmacokinetic assessment of the unbound brain-to-plasma disposition of eletriptan was conducted in wild type and mdr1a/1b knockout mice.
Results
We demonstrated that most triptans were able to inhibit uptake of the H+/OC antiporter substrate, pyrilamine, with eletriptan emerging as the strongest inhibitor. Eletriptan, almotriptan, and sumatriptan exhibited a pH-dependent uptake into hCMEC/D3 cells. Eletriptan demonstrated saturable uptake kinetics with an apparent Km of 89 ± 38 µM and a Jmax of 2.2 ± 0.7 nmol·min− 1·mg protein− 1 (n = 3). Bidirectional transport experiments across IPEC-J2 MDR1 monolayers showed that eletriptan is transported by P-gp, thus indicating that eletriptan is both a substrate of the H+/OC antiporter and P-gp. This was further confirmed in vivo, where the unbound brain-to-unbound plasma concentration ratio (Kp,uu) was 0.04 in wild type mice while the ratio rose to 1.32 in mdr1a/1b knockout mice.
Conclusions
We have demonstrated that the triptan family of compounds possesses affinity for the H+/OC antiporter proposing that the putative H+/OC antiporter plays a role in the BBB transport of triptans, particularly eletriptan. Our in vivo studies indicate that eletriptan is subjected to simultaneous brain uptake and efflux, possibly facilitated by the putative H+/OC antiporter and P-gp, respectively. Our findings offer novel insights into the potential central site of action involved in migraine treatment with triptans and highlight the significance of potential transporter related drug-drug interactions.
How much would Huey Long's Share Our Wealth Plan have lowered inequality?
Authors: Mathy Gabriel 
Published: March 2024

Abstract
Huey Long proposed a radical plan for wealth and income distribution, called the “Share Our Wealth” Plan. This was intended to end the Great Depression by imposing 100% taxes on income and wealth above a cap and to use that revenue to provide for a guaranteed minimum income and wealth, among other policies. I estimate the impact of these policies had they been implemented in a counterfactual United States of 1934. The largest impact on inequality comes from the cap on wealth, which makes the wealth of the very rich both lower and perfectly equal. However, wealth and income ceilings do nothing to reduce poverty or to improve the condition of the non-rich. Minimum income and wealth floors reduce inequality much less, but are much more effective at reducing poverty. However, the minimum income and wealth policies would require significant revenues to implement, which could not be funded by maximum income or wealth caps, which would raise little revenue.
JEL Codes: D31, D63, H23
The use of technology by seniors with neurocognitive disorders in long-term care: A scoping review
Authors: Hardy Marie-Soleil , Fanaki Chaimaa , Savoie Camille 
Published: March 2024

Abstract

Background To investigate and describe the current state of knowledge about the use of technology with seniors with neurocognitive disorders in long-term care to foster interactions, wellness, and stimulation. Methods Cumulative Index to Nursing and Allied Health Literature (CINAHL Plus); MEDLINE; PsycINFO; Embase and Web of Science were searched in eligible literature, with no limit of time, to describe the current use of technology by seniors with neurocognitive disorders in long-term care. All types of literature were considered except for theses, editorial, social media. Review Methods: This scoping review was built around the recommendations of Peters et al. (2020 version). Three researchers collaborated on the selection of articles and independently reviewed the papers, based on the eligibility criteria and review questions. Results The search yielded 3,605 studies, of which 39 were included. Results are presented in an overall description of the included studies encompassing study designs, population characteristics, and the current state of knowledge about different types of technologies used in long term care and the main findings on their use, objectives, and potential effects. Conclusion The study highlights different types and potential benefits of technology for long-term care residents with neurocognitive disorders, emphasizing the crucial need for additional research to refine interventions and their use.
Medical Tattooing as a Complementary Cosmetic Intervention to Reduce Body-Image Distress and Mental Health Symptoms in Breast Cancer Survivors
Authors: Proctor Miranda , Cassisi Jeffrey E. , Dvorak Robert , Decker Veronica 
Published: March 2024

Abstract

Purpose
A review of the literature revealed a high incidence of body-image distress among breast cancer survivors who had surgery. This study examined medical tattooing as a complementary intervention to mitigate body-image distress and poor mental health outcomes among breast cancer survivors following surgery.
Methods
We examined 330 responses collected through a nationwide online survey, pursuing two main objectives. First, we investigated body-image distress, depression and anxiety symptoms, and perceived stress in survivors who underwent breast cancer surgery, comparing those with medical tattooing (n = 89) and those without (n = 226). Second, we assessed the influence of the participant's surgery type on body-image distress, depression and anxiety symptoms, and perceived stress. Additionally, we evaluated whether individual factors, such as appearance investment, satisfaction with decision, and cosmetic expectation discrepancy, predicted the participant’s body-image distress.
Results
Findings suggest that participants with medical tattoos reported significantly lower body-image distress, depression and anxiety symptoms, and perceived stress compared to those without medical tattoos. The participant’s surgery type did not significantly predict body-image distress, depression or anxiety symptoms, or perceived stress. However, participants who reported greater appearance investment endorsed higher body-image distress. Participants who reported higher satisfaction with their treatment decisions and lower cosmetic expectation discrepancy endorsed lower body-image distress.
Conclusion
Medical tattooing can be a valuable tool in improving body-image distress and mental health. Empirical studies supporting the mental health benefits of medical tattooing among survivors is crucial to standardize insurance coverage and promote its inclusion as a complementary intervention across insurance providers nationwide. This complementary intervention should be considered using a patient-centered approach that aligns with the patient’s values and preferences.
The Effect of Teachers Beliefs and Experiences on the Use of ChatGPT in STEM Disciplines
Authors: Beege Maik , Hug Christopher , Nerb Josef 
Published: March 2024

Abstract
The rapid development of AI in the recent years has triggered numerous discussions in the education sector. AI offers numerous opportunities, but also challenges, especially for teachers. This study therefore examines the extent to which STEM teachers assess the benefits but also the risks of ChatGPT and how this influences the future use of AI in an educational context. As part of a questionnaire study, the current and future use of ChatGPT and various teacher variables were recorded and relationships between the variables were explored using a path model. In general, AI is still used relatively rarely, but future usage expectations are high compared to current usage. Results further revealed that perceived competence and benefits of ChatGPT have a positive influence on the use and intention to use it. Perceived risks and concerns have no significant influence on the usefulness of ChatGPT in the classroom, indicating that STEM teachers use AI in the classroom despite potential concerns and perceived risks. Additionally, perceived benefits and risks are negatively associated with each other, indicating that teachers rely on an affect heuristic when judging the usefulness of AI technology in the classroom.
Alternative culture medium for Nannochloropsis oculata mass culture
Authors: Neves Fábio de Farias , Sales Rafael de Oliveira Jaime , Ikeda Isadora Kaniak , Santos Ana Carolina de Souza , Duarte Ana Flavia Celso , Martins Ricardo Camilo , Schneider Rosana de Cássia de Souza , Willemann Daniel Pedro 
Published: March 2024

Abstract
Microalgae of the genus Nannochloropsis are highly valued in aquaculture due to their protein, fatty acid, and carotenoid content, offering potential as additives or substitutes for fish meal or oil. However, large-scale production encounters challenges, particularly concerning the culture medium. Thus, this study aimed to assess the impact of substituting artificial sea salt with common salt and standard medium with agricultural fertilizer in Nannochloropsis oculata production. Initially, the effects of reduced salinity were evaluated at salinities of 30, 10, 5, and 1 ups, followed by an examination of the effects of replacing artificial sea salt with common salt and using agricultural fertilizer on microalgae growth. Salinities of 30 and 10 exhibited the highest growth parameters. The salt source had no significant impact on culture growth, while the agricultural fertilizer enhanced it. Common salt increased the concentration of eicosapentaenoic acid compared to artificial sea salt, although the total lipid concentration was higher in microalgae cultured with artificial sea salt. The developed medium was validated through semi-continuous cultures in 100 L flat vertical bags, showing to be an economically viable alternative without hindering N. oculata growth.
Concurrent Validity and Responsiveness of Clinical Upper Limb Outcome Measures and Sensor-based Arm Use Metrics within the First Year after Stroke: A Longitudinal Cohort Study
Authors: Pohl Johannes , Verheyden Geert , Held Jeremia Philipp Oskar , Luft Andreas , Awai-Easthope Chris , Veerbeek Janne Marieke 
Published: March 2024

Abstract

Background: Concurrent validity and responsiveness of upper limb outcome measures are essential to interpret motor recovery poststroke. Evaluating the associations between clinical upper limb measures and sensor-based arm use (AU) fosters a comprehensive understanding of motor recovery. Defining sensor-based AU metrics for intentional upper limb movements could be crucial in mitigating bias arising from walking-related activities. Here, we investigate the measurement properties of a comprehensive set of clinical measures and sensor-based AU metrics when gait and non-functional upper limb movements are excluded.
Methods: In a prospective, longitudinal cohort study, individuals with motor impairment were measured at days 3±2 (D3), 10±2 (D10), 28±4 (D28), 90±7 (D90), and 365±14 (D365) after their first stroke. Upper limb function, activity capacity, and performance were assessed using the Fugl-Meyer Assessment, Action Research Arm Test, Box & Block Test, and the 14-item Motor Activity Log. For three days, individuals wore five movement sensors (trunk, wrists, and ankles). Thirteen AU metrics were computed based on functional movements during non-walking periods. Concurrent validity across clinical and AU metrics was determined by Spearman's rank correlations for each time point. Criterion-based responsiveness was examined by correlating patient-reported Global Rating of Perceived Change (GRPC) scores (1-7) and observed change in upper limb outcome. Optimal cut-off values for minimal important change (MIC) were estimated by ROC curve analysis.
Results: Ninety-three individuals participated. At D3 and D10, correlations between clinical measures and AU-metrics presented variability (range rs 0.44-0.90, p<0.01). All time points following showed strong positive associations between capacity measures and affected AU metrics (range rs 0.73-0.94, p<0.01), whereas unilateral nonaffected AU metrics had low-to-high negative associations (range rs 0.48-0.77). Responsiveness across outcomes was highest between D10-D28 within moderate-to-strong relations between GRPC and clinical measures (rs range 0.60-0.73, p<0.01), whereas relations were weaker for AU-metrics (rs range 0.28-0.43, p<0.05).
Eight MIC values were estimated for clinical measures and nine for AU metrics, showing moderate to good accuracy (66-87%).
Conclusions: We present reference data on concurrent validity and responsiveness of clinical upper limb measures and specified AU metrics within the first year poststroke. Estimated MIC values can be used as a benchmark for clinical stroke rehabilitation.
Trial registration: This trial was registered on clinicaltrials.gov; registration number NCT03522519.
Comparison of the ability of different quantitative indices in 123I-FP-CIT single-photon emission computed tomography to differentiate dopaminergic neurodegenerative disease
Authors: Sato Tomohiro , Sawai Setsu , Shimada Naokazu 
Published: March 2024

Abstract
Purpose By imaging dopamine transporter (DAT) uptake in the striatum, 123I-2β-carbomethoxy-3β-(4-iodophenyl)-N-(3-fluoropropyl) nortropane (123I-FP-CIT) single-photon emission computed tomography (SPECT) can differentiate dopaminergic neurodegenerative disease (dNDD) and non-dNDD, which differ in pathophysiology and clinical management. dNDD can be diagnosed by visual judgment and/or objective assessment using quantitative indices. Our aim was to compare and validate the diagnostic abilities of various 123I-FP-CIT SPECT quantitative indices for dNDD.
Methods Distribution volume ratio (DVR) and binding ratio (BR), measures of DAT uptake capacity, were measured by analyzing clinical 123I-FP-CIT SPECT images of 29 patients with dNDD, including dementia with Lewy bodies and Parkinson’s disease, and 18 patients with non-dNDD, using Montreal Neurological Institute space-based anatomical standardization and an atlas template, which utilizes statistical parametric mapping. Additionally, we computed the specific binding ratio (SBR) based on Bolt’s method and the maximum and mean standardized uptake values (SUVmax and SUVmean, respectively).
Results The caudate-to-occipital lobe, putamen-to-occipital lobe, and striatum-to-occipital lobe ratios (COR, POR, and SOR, respectively) on DVR and POR and SOR on BR were significantly lower in dNDD than in non-dNDD, with areas under the receiver operating curve (AUCs) of 0.941–0.960, showing high diagnostic accuracy for dNDD. However, the AUC of COR on BR was 0.839, indicating lower diagnostic performance. SBR had an AUC of 0.921, while SUVmax and SUVmean had AUCs of 0.906 and 0.900, respectively. Although striatal asymmetry on both DVR and BR exhibited AUCs of 0.728 and 0.734 and asymmetry on SBR showed an AUC of 0.757, the ratio-based DAT quantitative indices were superior. There were strong positive correlations of DVR with BR, DVR with SBR or SUVmax, BR with SBR or SUVmax, and SBR with SUVmax.
Conclusions COR, POR, and SOR on DVR and POR and SOR on BR were the most useful DAT quantitative indices. These indices can be compared with SBR and SUV, suggesting that comprehensive evaluation improves the diagnostic accuracy of dNDD.