bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA-free and genotype-independent CRISPR/Cas9 system in soybean
Authors: Kuwabara Chikako , Miki Ryuji , Maruyama Nobuyuki , Yasui Masanori , Hamada Haruyasu , Nagira Yozo , Imai Ryozo , Taoka Naoaki , Yamada Tetsuya 
Published: April 2024

AbstractThe CRISPR/Cas9 system has revolutionized the field of plant genetic engineering. Here we report a smart genome editing system of soybean by using iPB-RNP method without introducing foreign DNA and requiring traditional tissue culture processes such as embryogenesis and organogenesis. Shoot apical meristem (SAM) of embryonic axes was used the target tissue for genome editing, because the SAM in soybean mature seeds has stem cells and specific cell layer developing germ cells during reproductive growth stage. In the iPB-RNP method, the complex of ribonucleoprotein (RNP) and Cas9 protein was directly delivered into SAM stem cells via particle bombardment and genome-edited plants were generated from these SAMs. Soybean allergenic geneGly m Bd 30K, which we previously generated genome-editing soybean by usingAgrobacterium-mediated transformation and particle bombardment in our previous studies, was targeted in this study. Many E0(the first generation of genome-edited) plants in this experiment harbored mutant alleles at the targeted locus. Editing frequency of inducing mutations transmissible to the E1generation was approximately 0.4 to 4.6 % of all E0plants utilized in various soybean varieties. Furthermore, Gly m Bd 30K protein in mature seeds was not detected by western blot analysis due to flame-sift mutations. Our results offer a practical approach for both plant regeneration- and DNA-free genome editing achieved by delivering RNP into the SAM of dicotyledonous plants.
Kidney toxicology of a novel compound Lithium Bis(trifluoromethanesulfonyl)imide (LiTFSI, ie. HQ-115) used in energy applications: an Epigenetic evaluation
Authors: Sands Mia , Zhang Xing , Irudayaraj Joseph 
Published: April 2024

ABSTRACTExposure to emerging energy-based environmental contaminants such as lithium bis(trifluoromethanesulfonyl)imide (LiTFSI), more commonly known as HQ-115, poses a significant threat to human health, yet its impact on kidney function and epigenetic regulation remains poorly understood. Here, we investigated the effects of LiTFSI exposure on kidney-related biochemical parameters, renal injuries, and epigenetic alterations in male CD-1 mice under both 14-day and 30-day exposure durations. Our study revealed that LiTFSI exposure led to changes in kidney-related biochemical indicators, notably affecting serum bicarbonate levels, while relative kidney weight remained unaffected. Histological analysis unveiled tubule dilation, inflammation, and loss of kidney structure in LiTFSI-exposed mice, alongside dysregulated expression of genes associated with inflammation, renal function, and uric acid metabolism. Epigenetic analysis further identified widespread DNA methylation changes in the two exposure regimes. Functional analysis revealed that differentially methylated regions are implicated in cell apoptosis and cancer-related pathways and are enriched with development-related transcription factor binding motifs, suggesting a potential mechanism of action that can lead to kidney injury. These findings underscore the intricate interplay between environmental exposures, epigenetic modulation, and kidney health, emphasizing the need for additional research to unravel precise mechanisms that can help in the development of targeted interventions to mitigate the adverse effects of LiTFSI exposure on human health.SYNOPSISLiTFSI (HQ-115), an emerging environmental contaminant, impacts kidney health in male CD-1 mice by altering biochemical indicators, to result in renal injuries, and inducing epigenetic changes, highlighting environmental health concerns.
The flavivirus protein NS4B recruits thecis-Golgi protein ACBD3 to modify ER-Golgi trafficking for virion release
Authors: Yau Wai-Lok , Peters Marie B. A. , Sorin Marie , Rönfeldt Sebastian , Pulkkinen Lauri I. A. , Carlson Lars-Anders , Överby Anna K. , Lundmark Richard 
Published: April 2024

AbstractFlavivirus infection involves extensive remodeling of the endoplasmic reticulum (ER), which is key to both the replication of the viral RNA genome as well as the assembly and release of new virions. Yet, little is known about how viral proteins and host factors cooperatively facilitate such a vast transformation of the ER, and how this influences the different steps of the viral life cycle. In this study, we screened for host proteins that interact with the tick-borne encephalitis virus (TBEV) protein NS4B and found that the top candidates were coupled to trafficking between ER exit sites (ERES) and the Golgi. We characterized the role of ACBD3, one of the identified proteins, in flavivirus infection and show that it interacts with NS4B to promote infection across multiple flavivirus species. Using ACBD3 knockout cells, we found that the depletion of ACBD3 inhibited TBEV replication by preventing the trafficking of virions from the cell. We found that ACBD3 promotes flavivirus infection via a different mechanism than its previously described role in picornavirus infection. ACBD3 was enriched at modified ERES-Golgi contact sites to support virus replication. Therefore, we propose that ACBD3 promotes flavivirus replication by modifying the trafficking between the ERES and the Golgi to enable the release of new virions.Author summaryFlaviviruses including dengue virus and tick-borne encephalitis virus are group of viruses that widely affecting the health of human. During infection, flavivirus particles enter host cells and transform the endoplasmic reticulum (ER), which is the main structure for protein synthesis in cells. New flaviviral particles are produced in the transformed ER and then released to the Golgi apparatus, which is the main structure for protein transport in cells. It is unclear how the particles are transported from the ER to Golgi. Here we screened the factors that interact with viral proteins and identified a Golgi protein called ACBD3 as an important factor supporting flavivirus particles release from ER to Golgi. We showed that ACBD3 is recruited by flavivirus to a modified connection between ER and Golgi for viral particles release from the ER. Our work provides new insights into the fine coordination of virus replication and viral particles transport between organelles inside host cells.
Navigating the generative AI travel landscape: the influence of ChatGPT on the evolution from new users to loyal adopters
Authors: Li Yu , Lee Soyeun Olivia 
Published: April 2024

Purpose
This study, rooted in affordance-actualization theory and communication theory, aims to critically examine how ChatGPT influences users’ transition from new adopters to loyal advocates within the context of travel decision-making. It incorporates constructs including communication quality, personalization, anthropomorphism, cognitive and emotional trust (ET), loyalty and intention to adopt into a comprehensive model.

Design/methodology/approach
This study used quantitative methods to analyze data from 477 respondents, collected online through a self-administered questionnaire by Embrain, a leading market research company in South Korea. Lavaan package within R studio was used for evaluating the measurement model through confirmatory factor analysis and using structural equation modeling to examine the proposed hypotheses.

Findings
The findings reveal a pivotal need for enhancing ChatGPT’s communication quality, particularly in terms of accuracy, currency and understandability. Personalization emerges as a key driver for cognitive trust, while anthropomorphism significantly impacts ET. Interestingly, the study unveils that in the context of travel recommendations, users’ trust in ChatGPT predominantly operates at the cognitive level, significantly impacting loyalty and subsequent adoption intentions.

Practical implications
The findings of this research provide valuable insights for improving Generative AI (GenAI) technology and management practices in travel recommendations.

Originality/value
As one of the few empirical research papers in the burgeoning field of GenAI, this study proposes a highly explanatory model for the process from affordance to actualization in the context of using ChatGPT for travel recommendations.
Proposing a corporate governance model for an Islamic property developer
Authors: Puspitasari Novi , Mufidah Ana , Prihatini Dewi , Muhsyi Abdul , Suroso Imam 
Published: April 2024

Purpose
The purpose of this study include analyzing the conformity between the General Guidelines for the Governance of the Indonesian Sharia Entities (GGG-ISE) and the implementation in the field and proposing a model of corporate governance for Islamic property developers.

Design/methodology/approach
This research uses a qualitative method with a case study approach. The researcher used a structured interview method and chose a purposive technique to determine the interviewees. This study has seven interviewees representing three Islamic property developer companies in Jember Regency, East Java, Indonesia. Data collection was conducted from June to July 2023, with a duration of about 60 min for each interviewee. The interviews were conducted face-to-face in each interviewee’s residential office.

Findings
The results showed that the companies had implemented several principles of GGG-ISE, namely, ethical and responsible actors, risk management, internal control, compliance, disclosure and transparency by making financial reports, shareholder rights and stakeholder rights, both internal and external stakeholders. Furthermore, this study found that GGG-ISE does not comply with the components of the organizing organ group. This study also found that governance reports have not been implemented in GGG-ISE components. In addition, this study identified a new component that must be present and not found in GGG-ISE, namely, a statement of the use of contracts for mudharib owners and between mudharib owners and stakeholders. Based on these findings, this study proposes a governance model for Islamic property developer companies called the GGG-IPDE.

Originality/value
This research is a pioneer in proposing a corporate governance model for Islamic property developers.
The determinants of renting intention and renting satisfaction: the role of housing knowledge, housing challenges, and housing policy
Authors: Yaaco Mahazril ‘Aini , Hammad Ahmad Khan Hafizah , Mohd Noor Nurul Hidayana 
Published: April 2024

Purpose
This study aims to investigate the impact of housing knowledge, housing challenges and housing policy on the renting intention and satisfaction of young people.

Design/methodology/approach
A questionnaire survey helped collect data from young people in the study area, which were then analysed using the Statistical Package for the Social Sciences (SPSS) 27 software. A descriptive analysis and the Cronbach’s alpha test were adopted to analyse the data. The confirmatory factor analysis confirmed a significant relationship between housing knowledge, housing challenges and housing policy and renting intention and satisfaction.

Findings
The overall findings revealed that most young people intend to own a home one day, and a minority of them decided to continue renting. The findings suggest that there is a significant relationship between housing knowledge and housing intention. However, housing challenges and housing policies do not appear to impact renting intentions. On the other hand, housing knowledge and housing challenges were found to be associated with housing satisfaction, while housing policy does not show a significant relationship.

Research limitations/implications
This study, however, poses limitations as it uses a limited model and location and involves only a cross-sectional study. Future studies can use the methodology used in this study to conduct further investigations on housing intention and satisfaction in other regions of the country, thereby validating the findings of this study.

Practical implications
In terms of practical implications, this study has made a valuable contribution to the field of housing literature by shedding light on two crucial elements, namely, housing intention and satisfaction, which have been understudied. Understanding the determinants of housing intention and satisfaction is vital in efforts to implement appropriate policy reforms.

Social implications
Findings from this study offer valuable insight related to managerial and practical implications, with the former implicating a need to prioritise initiatives that enhance renters’ housing knowledge. Implementing educational programmes and providing accessible resources can empower renters with a better understanding of the rental process and other important housing information.

Originality/value
This paper is relevant because it provides a guideline for policymakers to initiate regulations concerning housing and implement appropriate policy reforms. This study can also help housing providers develop more affordable housing that meets the needs of young people currently renting because most have expressed their housing intentions. Understanding housing intention and satisfaction determinants is vital to implementing appropriate policy reforms.
An unequal distribution of the human right to health: assessing ethnic health disparities in cardiometabolic disease prevalence among Hawaii’s houseless population
Authors: Fancher Nicholas , Saha Bibek , Young Kurtis , Corpuz Austin , Cheng Shirley , Fontaine Angelique , Schiff-Elfalan Teresa , Omori Jill 
Published: April 2024

Purpose
In the state of Hawaii, it has been shown that certain ethnic minority groups, such as Filipinos and Pacific Islanders, suffer disproportionally high rates of cardiovascular disease, evidence that local health-care systems and governing bodies fail to equally extend the human right to health to all. This study aims to examine whether these ethnic health disparities in cardiovascular disease persist even within an already globally disadvantaged group, the houseless population of Hawaii.

Design/methodology/approach
A retrospective chart review of records from Hawaii Houseless Outreach and Medical Education Project clinic sites from 2016 to 2020 was performed to gather patient demographics and reported histories of type II diabetes, obesity, hyperlipidemia, hypertension and other cardiovascular disease diagnoses. Reported disease prevalence rates were compared between larger ethnic categories as well as ethnic subgroups.

Findings
Unexpectedly, the data revealed lower reported prevalence rates of most cardiometabolic diseases among the houseless compared to the general population. However, multiple ethnic health disparities were identified, including higher rates of diabetes and obesity among Native Hawaiians and other Pacific Islanders and higher rates of hypertension among Filipinos and Asians overall. The findings suggest that even within a generally disadvantaged houseless population, disparities in health outcomes persist between ethnic groups and that ethnocultural considerations are just as important in caring for this vulnerable population.

Originality/value
To the best of the authors’ knowledge, this is the first comprehensive study focusing on ethnic health disparities in cardiovascular disease and the structural processes that contribute to them, among a houseless population in the ethnically diverse state of Hawaii.
Lean six sigma 4.0 methodology for optimizing occupational exams in operations management
Authors: Moreira Tatiana da Costa Reis , Nascimento Daniel Luiz de Mattos , Smirnova Yelena , Santos Ana Carla de Souza Gomes dos 
Published: April 2024

Purpose
This paper explores Lean Six Sigma principles and the DMAIC (define, measure, analyze, improve, control) methodology to propose a new Lean Six Sigma 4.0 (LSS 4.0) framework for employee occupational exams and address the real-world issue of high-variability exams that may arise.

Design/methodology/approach
This study uses mixed methods, combining qualitative and quantitative data collection. A detailed case study assesses the impact of LSS interventions on the exam management process and tests the applicability of the proposed LSS 4.0 framework for employee occupational exams.

Findings
The results reveal that changing the health service supplier in the explored organization caused a substantial raise in occupational exams, leading to increased costs. By using syntactic interoperability, lean, six sigma and DMAIC approaches, improvements were identified, addressing process deviations and information requirements. Implementing corrective actions improved the exam process, reducing the number of exams and associated expenses.

Research limitations/implications
It is important to acknowledge certain limitations, such as the specific context of the case study and the exclusion of certain exam categories.

Practical implications
The practical implications of this research are substantial, providing organizations with valuable managerial insights into improving efficiency, reducing costs and ensuring regulatory compliance while managing occupational exams.

Originality/value
This study fills a research gap by applying LSS 4.0 to occupational exam management, offering a practical framework for organizations. It contributes to the existing knowledge base by addressing a relatively novel context and providing a detailed roadmap for process optimization.
Service user perspectives on recovery: the construction of unfulfilled promises in mental health service delivery in Ireland
Authors: Swords Calvin , Houston Stan 
Published: April 2024

Purpose
The concept of personal recovery is now a key pillar of service delivery. It aims to support individuals to flourish and establish a new identity following an acute episode or diagnosis. This view of recovery is unique to each person on that journey. However, there has been a significant focus on measuring these experiences. This paper aims to explore the influence of social constructionism on the concept of recovery within an Irish context, seeking to understand the influence of language, discourse and power on service users’ experiences.

Design/methodology/approach
A qualitative, interpretivist methodology was adopted for this case study design. Semi-structured interviews were conducted with 12 service users. Thematic analysis was chosen as the method of analysis.

Findings
Personalising recovery did not always lead to the removal of biological symptoms, but with the appropriate supports, individual’s recovery journey was greatly enhanced. On the contrary, personal recovery places overwhelmingly responsibility on the individual to succeed, largely driven by neoliberal discourse. This focus on individualism and the pressure to succeed was further experienced when people sought to re-integrate into society and participate in normalised social order. Ultimately, for many service users, they viewed personal recovery as an unfulfilled promise.

Research limitations/implications
It is not a representative sample of service users within an Irish context.

Originality/value
To the best of the authors’ knowledge, this is the first study to explore influence of social constructionism on the concept of personal recovery within a mental health service context.
Ethical leadership behaviors are their own reward for leaders: a moderated mediation model
Authors: Xu Zhixing , Ju Dong 
Published: April 2024

PurposeThis study investigates the benefits of ethical leadership behaviors for leaders themselves and the underlying mechanisms and boundary conditions of this effect.Design/methodology/approachUsing a multi-time and multi-source survey design, data was collected from both leaders and subordinates across three waves.FindingsEthical leadership behavior was found to be positively associated with the leader’s moral pride, resulting in the leader’s higher in-role performance and perceived manager effectiveness. The effect of ethical leadership behavior was moderated by core self-evaluation (CSE), such that low-CSE leaders benefit more from these behaviors.Practical implicationsOrganizations should encourage ethical leadership behaviors and educate leaders to develop moral pride from conducting these behaviors. Leaders with low CSEs can enhance their in-role performance and overall effectiveness by taking pride in their ethical leadership behaviors.Originality/valueThe field of study on ethical leadership has predominantly focused on the positive outcomes for recipients, yet it is imperative to examine the self-benefits for leaders as well. This study drew upon affective events theory to posit that ethical leadership behaviors generate moral pride in leaders, leading to improved work-related attitudes and performance outcomes.
Is ChatGPT-like technology going to replace commercial search engines?
Authors: Strzelecki Artur 
Published: April 2024

Purpose
This paper aims to give an overview of the history and evolution of commercial search engines. It traces the development of search engines from their early days to their current form as complex technology-powered systems that offer a wide range of features and services.

Design/methodology/approach
In recent years, advancements in artificial intelligence (AI) technology have led to the development of AI-powered chat services. This study explores official announcements and releases of three major search engines, Google, Bing and Baidu, of AI-powered chat services.

Findings
Three major players in the search engine market, Google, Microsoft and Baidu started to integrate AI chat into their search results. Google has released Bard, later upgraded to Gemini, a LaMDA-powered conversational AI service. Microsoft has launched Bing Chat, renamed later to Copilot, a GPT-powered by OpenAI search engine. The largest search engine in China, Baidu, released a similar service called Ernie. There are also new AI-based search engines, which are briefly described.

Originality/value
This paper discusses the strengths and weaknesses of the traditional – algorithmic powered search engines and modern search with generative AI support, and the possibilities of merging them into one service. This study stresses the types of inquiries provided to search engines, users’ habits of using search engines and the technological advantage of search engine infrastructure.