bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single pediatric center experience with multiple device implantation for complex secundum atrial septal defects
Authors: Bramlet Matthew T. , Hoyer Mark H. 
Published: September 2008

AbstractObjectives: We analyzed the efficacy and complications of placing multiple transcatheter devices to correct complex ASD in a primarily pediatric population. Background: Limited pediatric information exists regarding the safety and efficacy of using multiple devices for secundum atrial septal defects (ASD) when multiple defects are present. Methods: Cath lab, echo, and clinical data were reviewed for 238 patients who underwent device closure of secundum ASD from 2000 to 2007 at a single pediatric center. Of those requiring multiple devices (n = 15), residual shunting and complications were assessed. Results: 238 patients (mean age 12 yrs; 80% < 16 yrs) underwent transcatheter ASD closure. 34 (14%) had multiple ASD, but only 15 received multiple devices (2 devices in 14 and 3 devices in 1). The mean age and weight (12 yrs; 35 kg) represent a younger population than previously reported. Only 3 were > 15 yrs, with a median age of 10.8 yrs (range 2–31). There was no compromise or obstruction to surrounding structures, and no device embolization or erosion was noted. A tiny thrombus was observed on the right sided screw of one device with no clinical sequelae. One patient (age 31 yrs) died at home 30 days post procedure from cardiac tamponade. Autopsy revealed the 3 intact devices in stable position without evidence of erosion. Latest available echo follow‐up for the group revealed functional resolution of atrial level shunting in all patients. Conclusion: Multiple devices can safely and effectively be implanted in the pediatric population to close complex ASD. © 2008 Wiley‐Liss, Inc.
Slipping into trance
Authors: Kirsch Irving , Mazzoni Giuliana , Roberts Kathrine , Dienes Zoltan , Hallquist Michael N. , Williams John , Lynn Steven Jay 
Published: September 2008

AbstractIt has been hypothesized that highly hypnotizable people spontaneously slip into trance when given imaginative suggestions without prior induction of hypnosis. We tested this in two studies. In Study 1, we examined state reports from ten highly suggestible students following the administration of a suggestion for altered colour perception. The suggestion was administered twice, once with and once without prior induction of hypnosis. Students reported equivalent perceptual changes with and without the induction of hypnosis, but reported being in a hypnotic state only when a hypnotic induction had been administered. In Study 2, participants received either a hypnotic induction or specific suggestions to not slip into hypnosis. Even under these circumstances, subjective responding was equivalent in both conditions and behavioural responding was only slightly higher in the ‘hypnosis’ condition. These data disconfirm the slipping‐into‐hypnosis hypothesis. Copyright © 2008 British Society of Experimental & Clinical Hypnosis. Published by John Wiley & Sons, Ltd.
Sex‐specific expression of SOX9 during gonadogenesis in the amphibian Xenopus tropicalis
Authors: El Jamil Anwar , Kanhoush Rasha , Magre Solange , Boizet‐Bonhoure Brigitte , Penrad‐Mobayed May 
Published: September 2008

AbstractTo investigate the role of SOX9 gene in amphibian gonadogenesis, we analyzed its expression during male and female gonadogenesis in Xenopus tropicalis. The results showed that in both sexes SOX9 mRNA and protein were first detectable after metamorphosis when the gonads were well differentiated and remained present until the adult stage. In the testis, SOX9 expression was restricted to the nucleus of Sertoli‐like cells, similarly to what has been observed in other vertebrates suggesting a conserved role in vertebrate testicular differentiation. In the ovary, in sharp contrast with what has been observed in all vertebrates examined so far, the SOX9 protein was localized in the cytoplasm of previtellogenic oocytes before being translocated into the nucleus of vitellogenic oocytes suggesting an unexpected role during oogenesis. These results suggest that the SOX9 gene may not be a sex‐determining gene in X. tropicalis and may play different functions in testicular and ovarian differentiation. Developmental Dynamics 237:2996–3005, 2008. © 2008 Wiley‐Liss, Inc.
Development of lymphatic vessels in mouse embryonic and early postnatal hearts
Authors: Juszyński Michał , Ciszek Bogdan , Stachurska Emilia , Jabłońska Anna , Ratajska Anna 
Published: September 2008

AbstractWe aimed to study the spatiotemporal pattern of lymphatic system formation in the embryonic and early postnatal mouse hearts. The first sign of the development of lymphatics are Lyve‐1–positive cells located on the subepicardial area. Strands of Lyve‐1–positive cells occur first along the atrioventricular sulcus of the diaphragmatic surface and then along the great arteries. Lumenized tubules appear, arranged in rows or in a lattice. They are more conspicuous in dorsal atrioventricular junction, along the major venous and coronary artery branches and at the base of the aorta and the pulmonary trunk extending toward the heart apex. At later stages, some segments of the lymphatic vessels are partially surrounded by smooth muscle cells. Possible mechanisms of lymphangiogenesis are: addition of Lyve‐1–positive cells to the existing tubules, elongation of the lymphatic lattice, sprouting and coalescence of tubules. We discuss the existence of various subpopulations of endothelial cells among the Lyve‐1–positive cells. Developmental Dynamics 237:2973–2986, 2008. © 2008 Wiley‐Liss, Inc.
Thyroid hormone–up‐regulated hedgehog interacting protein is involved in larval‐to‐adult intestinal remodeling by regulating sonic hedgehog signaling pathway in Xenopus laevis
Authors: Hasebe Takashi , Kajita Mitsuko , Shi Yun‐Bo , Ishizuya‐Oka Atsuko 
Published: September 2008

AbstractSonic hedgehog (Shh) was previously shown to be involved in the larval‐to‐adult remodeling of the Xenopus laevis intestine. While Shh is transcriptionally regulated by thyroid hormone (TH), the posttranscriptional regulation of Shh signaling during intestinal remodeling is largely unknown. In the present study, we focused on a role of the pan‐hedgehog inhibitor, hedgehog interacting protein (Hip), in the spatiotemporal regulation of Shh signaling. Using real‐time reverse transcriptase‐polymerase chain reaction and in situ hybridization, we show that Hip expression is transiently up‐regulated during both natural and TH‐induced metamorphosis and that Hip mRNA is localized in the connective tissue adjacent to the adult epithelial primordia expressing Shh. Interestingly, the expression of bone morphogenetic protein‐4, a Shh target gene, is hardly detectable where Hip is strongly expressed. Finally, we demonstrate that Hip binds to the N‐terminal fragment of processed Shh in vivo, suggesting that Hip suppresses Shh signaling through sequestering Shh. Developmental Dynamics 237:3006–3015, 2008. © 2008 Wiley‐Liss, Inc.
Inhibition of mammalian muscle differentiation by regeneration blastema extract of Sternopygus macrurus
Authors: Kim Hyun‐Jung , Archer Eric , Escobedo Norma , Tapscott Stephen J. , Unguez Graciela A. 
Published: September 2008

AbstractTissue regeneration through stem cell activation and/or cell dedifferentiation is widely distributed across the animal kingdom. By comparison, regeneration in mammals is poor and this may reflect a limited dedifferentiation potential of mature cells. Because mammalian myotubes can dedifferentiate in the presence of newt blastema extract, the present study tested the dedifferentiation induction capability of the blastema from the teleost Sternopygus macrurus (SmBE). Our in vitro data showed that SmBE did not induce cell cycle reentry of myonuclei in myotubes. Instead, SmBE caused myotubes to detach and time‐lapse imaging analyses characterized the cellular events before their detachment. Furthermore, SmBE enhanced myoblast proliferation and reversibly inhibited their differentiation. These data suggest the presence of protein factors in SmBE that regulate mammalian muscle physiology and differentiation, but do not support the conservation of a dedifferentiation induction capability by the blastema of S. macrurus. Developmental Dynamics 237:2830–2843, 2008. © 2008 Wiley‐Liss, Inc.
Drosophila SIN3 is required at multiple stages of development
Authors: Sharma Vishal , Swaminathan Aishwarya , Bao Riyue , Pile Lori A. 
Published: September 2008

AbstractSIN3 is a component of a histone deacetylase complex known to be important for transcription repression. While multiple isoforms of SIN3 have been reported, little is known about their relative expression or role in development. Using a combination of techniques, we have determined that SIN3 is expressed throughout the Drosophila life cycle. The pattern of expression for each individual isoform, however, is distinct. Knock down of all SIN3 expression reveals a requirement for this protein in embryonic and larval periods. Taken together, the data suggest that SIN3 is required for multiple developmental events during the Drosophila life cycle. Developmental Dynamics 237:3040–3050, 2008. © 2008 Wiley‐Liss, Inc.
Kinetic and mechanical analysis of live tube morphogenesis
Authors: Cheshire Alan M. , Kerman Bilal E. , Zipfel Warren R. , Spector Alexander A. , Andrew Deborah J. 
Published: September 2008

AbstractRibbon is a nuclear Broad Tramtrack Bric‐a‐brac (BTB) ‐domain protein required for morphogenesis of the salivary gland and trachea. We recently showed that ribbon mutants exhibit decreased Crumbs and Rab11‐coincident apical vesicles and increased apical Moesin activity and microvillar structure during tube elongation. To learn how these molecular and morphological changes affect the dynamics of tubulogenesis, we optimized an advanced two‐photon microscope to enable high‐resolution live imaging of the salivary gland and trachea. Live imaging revealed that ribbon mutant tissues exhibit slowed and incomplete lumenal morphogenesis, consistent with previously described apical defects. Because Moesin activity correlates with cortical stiffness, we hypothesize that ribbon mutants suffer from increased apical stiffness during morphogenesis. We develop this hypothesis through mechanical analysis, using the advantages of live imaging to construct computational elastic and analytical viscoelastic models of tube elongation, which suggest that ribbon mutant tubes exhibit three‐ to fivefold increased apical stiffness and twofold increased effective apical viscosity. Developmental Dynamics 237:2874–2888, 2008. © 2008 Wiley‐Liss, Inc.
Development of diverse lateral line patterns on the teleost caudal fin
Authors: Wada Hironori , Hamaguchi Satoshi , Sakaizumi Mitsuru 
Published: September 2008

AbstractThe lateral line is composed of mechanoreceptors, the neuromasts, which are distributed over the body surfaces of fish. We examine the development of neuromast patterns on the caudal fins of medaka and zebrafish. In medaka, the terminal neuromast is established just prior to the caudal fin formation. The terminal neuromast subsequently gives rise to a cluster of accessory neuromasts. In zebrafish, the terminal neuromasts vary in terms of both number and position, and they achieve their final positions relative to the caudal fin structures through migration. Subsequently, they give rise to four lines of accessory neuromasts that extend along the caudal fin. We show that developmental processes similar to those observed in medaka and zebrafish may account for a large variety of patterns in other teleost species. These results establish terminal neuromast patterning as a new model for the study of the developmental mechanisms underlying diverse lateral line patterns. Developmental Dynamics 237:2889–2902, 2008. © 2008 Wiley‐Liss, Inc.
Perplexing Pax: From puzzle to paradigm
Authors: Blake Judith A. , Thomas Meghan , Thompson Jennifer A. , White Robert , Ziman Melanie 
Published: September 2008

AbstractPax transcription factors are critical for the development of the central nervous system (CNS) where they have a biphasic role, initially dictating CNS regionalization, while later orchestrating differentiation of specific cell subtypes. While a plethora of expression, misexpression, and mutation studies lend support for this argument and clarify the importance of Pax genes in CNS development, less well understood, and more perplexing, is the continued Pax expression in the adult CNS. In this article we explore the mechanism of action of Pax genes in general, and while being cognizant of existing developmental data, we also draw evidence from (1) adult progenitor cells involved in regeneration and tissue maintenance, (2) specific expression patterns in fully differentiated adult cells, and (3) analysis of direct target genes functioning downstream of Pax proteins. From this, we present a more encompassing theory that Pax genes are key regulators of a cell's measured response to a dynamic environment. Developmental Dynamics 237:2791–2803, 2008. © 2008 Wiley‐Liss, Inc.
Development of the proepicardium in Xenopus laevis
Authors: Jahr Maike , Schlueter Jan , Brand Thomas , Männer Jörg 
Published: September 2008

AbstractThe proepicardium (PE) is an embryonic progenitor cell population, which provides the epicardium, the majority of the cardiac interstitium, the coronary vasculature and possibly some cardiomyocytes. Recent studies have documented (1) the presence of bilaterally paired PE anlagen in several vertebrates, and (2) species‐specific differences in the fate of the left and right PE anlagen. Here, we document PE development in Xenopus laevis (stages 37–46). The PE appears at stage 41 in the form of a cone‐shaped accumulation of mesothelial cells covering the pericardial surface of the right horn of the sinus venosus. No such structure appears on the left sinus horn. At the end of stage 41, the tip of the PE establishes a firm contact with the developing ventricle. A secondary tissue bridge is established facilitating the transfer of PE cells to the heart. During stages 41–46, this tissue bridge is visible in vivo through the transparent body wall. Corresponding to the morphological data, the PE marker gene Tbx18 is expressed only on the right sinus horn suggesting a right‐sided origin of the PE. Left–right lineage tracing has confirmed this idea. These results show that Xenopus PE development proceeds in a bilaterally asymmetric pattern as previously observed in chicks. We speculate that asymmetric PE development is controlled by signals from left–right signaling pathways and that the PE is an indicator for right‐sidedness in Xenopus embryos. Xenopus might be a good model to uncover the role of left–right signaling pathways in the control of asymmetric PE development. Developmental Dynamics 237:3088–3096, 2008. © 2008 Wiley‐Liss, Inc.
VEGF‐mediated fusion in the generation of uniluminal vascular spheroids
Authors: Gentile Carmine , Fleming Paul A. , Mironov Vladimir , Argraves Kelley M. , Argraves W. Scott , Drake Christopher J. 
Published: September 2008

AbstractEmbryonic mouse allantoic tissue (E8.5) was cultured in hanging drops to generate a three‐dimensional vascular micro‐tissue. The resulting tissue spheroids had an inner network of small diameter vessels expressing platelet endothelial cell adhesion molecule‐1 (PECAM‐1) and an outer layer of cells expressing SMαA, SM22‐α, and SM‐MHC. In a subsequent phase of culture, the fusion‐promoting activity of vascular endothelial growth factor (VEGF) was used to transform the inner network of small diameter endothelial tubes into a contiguous layer of cells expressing PECAM‐1, CD34, and VE‐cadherin that circumscribed a central lumen‐like cavity. The blood vessel‐like character of the VEGF‐treated spheroids was further demonstrated by their physiologically relevant vasodilatory and contractile responses, including contraction induced by KCl and relaxation stimulated by high‐density lipoproteins and acetylcholine‐induced nitric oxide production. Developmental Dynamics 237:2918–2925, 2008. © 2008 Wiley‐Liss, Inc.
Leading edge‐secreted Dpp cooperates with ACK‐dependent signaling from the amnioserosa to regulate myosin levels during dorsal closure
Authors: Zahedi Baharak , Shen Weiping , Xu Xing , Chen Xi , Mahey Meena , Harden Nicholas 
Published: September 2008

AbstractDorsal closure of the Drosophila embryo is an epithelial fusion in which the epidermal flanks migrate to close a hole in the epidermis occupied by the amnioserosa, a process driven in part by myosin‐dependent cell shape change. Dpp signaling is required for the morphogenesis of both tissues, where it promotes transcription of myosin from the zipper (zip) gene. Drosophila has two members of the activated Cdc42‐associated kinase (ACK) family: DACK and PR2. Overexpression of DACK in embryos deficient in Dpp signaling can restore zip expression and suppress dorsal closure defects, while reducing the levels of DACK and PR2 simultaneously using mutations or amnioserosa‐specific knock down by RNAi results in loss of zip expression. ACK function in the amnioserosa may generate a signal cooperating with Dpp secreted from the epidermis in driving zip expression in these two tissues, ensuring that cell shape changes in dorsal closure occur in a coordinated manner. Developmental Dynamics 237:2936–2946, 2008. © 2008 Wiley‐Liss, Inc.
Analysis of the IKKβ/NF‐κB signaling pathway during embryonic angiogenesis
Authors: Hou Yanjun , Li Fu , Karin Michael , Ostrowski Michael C. 
Published: September 2008

AbstractThe nuclear factor‐κB (NF‐κB) signaling pathway regulates cellular growth, survival, differentiation and development. In this study, the functions of IκB kinase (IKK)β in angiogenesis during mouse development were examined. Conditional disruption of the Ikkβ locus in endothelial cells using the well‐characterized Tie2‐Cre transgene resulted in embryonic lethality between embryonic day (E) 13.5 and E15.5. Examination of the mutant embryos revealed that while deletion of Ikkβ occurred in endothelial cells throughout the embryo, only the vascular network in the fetal liver was affected. Disruption of the fetal liver vasculature was accompanied by decreased cell proliferation and increased apoptosis of hepatocytes, but hematopoiesis was not affected. Increased apoptosis was not observed outside of fetal liver in the mutant embryos. These results indicate that the IKKβ/NF‐κB pathway plays a previously unappreciated role in development of the sinusoidal vasculature in the fetal liver and additionally that this pathway is critical in the crosstalk between endothelial cells and hepatocytes during mouse development. Developmental Dynamics 237:2926–2935, 2008. © 2008 Wiley‐Liss, Inc.
Origin and fate of cardiac mesenchyme
Authors: Snarr Brian S. , Kern Christine B. , Wessels Andy 
Published: September 2008

AbstractThe development of the embryonic heart is dependent upon the generation and incorporation of different mesenchymal subpopulations that derive from intra‐ and extra‐cardiac sources, including the endocardium, epicardium, neural crest, and second heart field. Each of these populations plays a crucial role in cardiovascular development, in particular in the formation of the valvuloseptal apparatus. Notwithstanding shared mechanisms by which these cells are generated, their fate and function differ profoundly by their originating source. While most of our early insights into the origin and fate of the cardiac mesenchyme has come from experimental studies in avian model systems, recent advances in transgenic mouse technology has enhanced our ability to study these cell populations in the mammalian heart. In this article, we will review the current understanding of the role of cardiac mesenchyme in cardiac morphogenesis and discuss several new paradigms based on recent studies in the mouse. Developmental Dynamics 237:2804–2819, 2008. © 2008 Wiley‐Liss, Inc.
Fgf16 is required for cardiomyocyte proliferation in the mouse embryonic heart
Authors: Hotta Yuhei , Sasaki Sayaka , Konishi Morichika , Kinoshita Hideyuki , Kuwahara Koichiro , Nakao Kazuwa , Itoh Nobuyuki 
Published: September 2008

AbstractFibroblast growth factor (Fgf) signaling plays important roles in development and metabolism. Mouse Fgf16 was predominantly expressed in cardiomyocytes. To elucidate the physiological roles of Fgf16, we generated Fgf16 knockout mice. Although the mice were apparently normal and fertile, heart weight and cardiomyocyte cell numbers were slightly decreased at 6 months of age. However, blood pressure, heart rate, and cardiac performance were essentially unchanged. In addition, the expression of most cardiac marker genes examined was also essentially unchanged. However, the expression of Bnp was significantly decreased, indicating potential roles of Fgf16 in the heart under pathological conditions. In contrast, the proliferation of embryonic cardiomyocytes was significantly decreased, indicating that Fgf16 is a growth factor for these cells. The embryonic heart phenotype is similar to that of the Fgf9 knockout heart, indicating Fgf9 and Fgf16 to synergistically act as growth factors for embryonic cardiomyocytes. Developmental Dynamics 237:2947–2954, 2008. © 2008 Wiley‐Liss, Inc.
Ultrafast Vibronic Processes in a Ru–Porphyrin Complex
Authors: Iwakura Izumi , Yabushita Atsushi , Kobayashi Takayoshi 
Published: September 2008

AbstractThe excited‐state dynamics of RuII(TPP)(CO)(acetone) (TPP = tetraphenylporphyrin) was investigated by using a sub‐5‐fs pulse. In previous studies, the fluorescence lifetime of RuII(TPP)(CO) could not be resolved and was reported to be <1 ps. Analysis of the six‐coordinate complexes of RuII(TPP)(CO)(acetone) showed that 1Qx(π,π*) had a lifetime of 140 ± 20 fs and the lifetime of 3(d,π*) was 560 ± 150 fs. Stimulated emission due to a spin‐forbidden transition from 3(π,π*) to the ground state was observed for the first time.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Controlling the Crystal Growth of Dodecasil 3C by Buffering with DL‐Histidine
Authors: Seral José J. , Uriel Santiago , Coronas Joaquín 
Published: September 2008

AbstractDodecasil 3C, a pure silica zeolite with an MTN‐type structure, was prepared by liquid‐phase hydrothermal synthesis in the presence of the amino acid dl‐histidine. This compound acts as a buffer and affects the growth of zeolite, in particular its aggregation behaviour and particle size. As a result of the alteration the amino acid exerts on the silica chemistry in aqueous solution, nucleation was lowered and dodecasil 3C was formed as large, single crystals with sizes up to 140 μm.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Organometallic Diiron Complex Chemistry Related to the [2Fe]H Subsite of [FeFe]H2ase
Authors: Capon Jean‐François , Gloaguen Frédéric , Pétillon François Y. , Schollhammer Philippe , Talarmin Jean 
Published: September 2008

AbstractDuring the last decade, the structure of the subsite of [FeFe]H2ases (or Fe‐only hydrogenases) has inspired organometallic chemists to find out whether it was possible to build synthetic molecules, based on sulfur, iron, carbon monoxide and cyanide or surrogates, having the same efficiency for hydrogen production/uptake as that of the enzyme. A short nonexhaustive overview of recent advances in this chemistry is presented. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)