bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ligand binding properties of three odorant‐binding proteins in striped flea beetle Phyllotreta striolata towards two phthalate esters
Authors: Xiao Yong , Wu Yuhong , Lei Chunmei , Yin Fei , Peng Zhengke , Jing Xiangfeng , Zhang Yongjun , Li Zhenyu 
Published: March 2024

AbstractOdorant‐binding proteins (OBPs) initiate insect olfactory perception and mediate specific binding and selection of odorants via uncertain binding mechanisms. We characterized the binding characteristics of four OBPs from the striped flea beetle Phyllotreta striolata (SFB), a major cruciferous crop pest. Tissue expression analysis revealed that the two ABPII OBPs (PstrOBP12 and PstrOBP19) were highly expressed mainly in the antenna, whereas the two minus‐C OBPs (PstrOBP13 and PstrOBP16) showed a broad expression pattern. Competitive binding assays of cruciferous plant volatiles showed that PstrOBP12, PstrOBP16 and PstrOBP19 had very strong binding capacities for only two phthalate esters (Ki < 20 μM), and PstrOBP13 specifically bound to four aromatic volatiles (Ki < 11 μM). Fluorescence quenching assays displayed that two phthalate esters bound to three PstrOBPs via different quenching mechanisms. PstrOBP12/PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6‐methylheptyl) phthalate followed static quenching, while PstrOBP12/PstrOBP16–bis(6‐methylheptyl) phthalate and PstrOBP19–diisobutyl phthalate followed dynamic quenching. Homology modelling and molecular docking displayed that PstrOBP12–diisobutyl phthalate was driven by H‐bonding and van der Waals interactions, while PstrOBP16–diisobutyl phthalate and PstrOBP19–bis(6‐methylheptyl) phthalate followed hydrophobic interactions. Finally, behavioural activity analysis demonstrated that phthalate esters exhibited different behavioural activities of SFB at different doses, with low doses attracting and high doses repelling. Overall, we thus revealed the different binding properties of the three PstrOBPs to two phthalate esters, which was beneficial in shedding light on the ligand‐binding mechanisms of OBPs.
Prevention and treatment of HPV‐related cancer through a mRNA vaccine expressing APC‐targeting antigen
Authors: Li Xiaoxuan , Wang Huiyan , Lai Wujiang , Liao Jinrong , Mo Wenyu , Huang Keke , He Liqing , Liang Xiaomei , Yu Zhibin , Xu Jiang , Hua Xianwu , Hou Fujun , Ding Jun , Jia William Wei‐Guo , Zhang Kuan , Wang Yifeng 
Published: March 2024

AbstractPersistent human papillomavirus (HPV) infection is associated with multiple malignancies. Developing therapeutic vaccines to eliminate HPV‐infected and malignant cells holds significant value. In this study, we introduced a lipid nanoparticle encapsulated mRNA vaccine expressing tHA‐mE7‐mE6. Mutations were introduced into E6 and E7 of HPV to eliminate their tumourigenicity. A truncated influenza haemagglutinin protein (tHA), which binds to the CD209 receptor on the surface of dendritic cells (DCs), was fused with mE7‐mE6 in order to allow efficient uptake of antigen by antigen presenting cells. The tHA‐mE7‐mE6 (mRNA) showed higher therapeutic efficacy than mE7‐mE6 (mRNA) in an E6 and E7+ tumour model. The treatment resulted in complete tumour regression and prevented tumour formation. Strong CD8+ T‐cell immune response was induced, contributing to preventing and curing of E6 and E7+ tumour. Antigen‐specific CD8+ T were found in spleens, peripheral blood and in tumours. In addition, the tumour infiltration of DC and NK cells were increased post therapy. In conclusion, this study described a therapeutic mRNA vaccine inducing strong anti‐tumour immunity in peripheral and in tumour microenvironment, holding promising potential to treat HPV‐induced cancer and to prevent cancer recurrence.
Association between stress and catecholamines with painful TMD in children
Authors: Restrepo‐Serna Claudia , Santamaría‐Villegas Adriana 
Published: March 2024

AbstractBackgroundStudying biomarkers in children with temporomandibular disorders (TMD) such as epinephrine, norepinephrine, and dopamine may reveal factors like screen time or sleep loss that affect these biomarkers and predict TMD‐related pain, offering new research opportunities.AimTo determine the association between stress and catecholamines with myofascial pain and headache related to TMD in children.DesignSixty‐six 9‐ to 11‐year‐old children assisting at the clinics of Pediatric Dentistry of Universidad CES participated in the study. Myofascial pain and headache attributed to TMD were determined according to the Diagnostic Criteria for TMD (DC/TMD) Axis I. Stress was evaluated with the Perceived Stress Scale—Children (PSS‐C), and a 24‐h urine sample was analyzed using liquid chromatography to assay catecholamines. Single and multiple regression analyses were performed.ResultsChildren with a mean age of 10.3 years participated in the study. The mean score of stress was 29 ± 4. Perceived stress, dopamine, epinephrine, and norepinephrine were statistically significant predictors of myofascial pain and headache attributed to TMD in the single‐ and multiple variable logistic regression analyses.ConclusionStressful states and its biological biomarkers increase the probability of developing myofascial pain and headache attributed to TMD in children.
Thriving in a bruising job: How high achieving IT professionals can cope with occupational demands
Authors: Mattern Jana , Tarafdar Monideepa , Klein Stefan , Schellhammer Stefan 
Published: March 2024

AbstractWe report on a study of high performing IT professionals in a global IT services company, whose exceptional performance in a highly demanding work environment raises the question of how they cope with their occupational demands. While literature has focused primarily on technology‐induced stressors and associated coping behaviours of IT users, our study examines distinctive coping behaviours of IT professionals in response to diverse occupational demands. We combine qualitative interviews and heart rate variability data from an exemplar sample of 15 high performing IT professionals to provide insights into their psychological and physiological strain levels respectively. Our participants exhibit four strain levels, each related to a distinctive combination of coping behaviours, which we abductively theorise as coping portfolios. We find that high performing IT professionals with both a low psychological and physiological strain level apply a broad and varied portfolio of coping behaviours in response to diverse occupational demands. We contribute to IS research on IT professionals by studying the coping behaviours of an exemplar sample of high performing IT professionals in a leading IT firm. Theoretically, we complement the established concepts of coping flexibility and coping repertoires by introducing the notion of coping portfolios.
Influence of (Ta Nb Ti V) C powders synthesized by molten salt at low temperature on densification and properties of high entropy carbide ceramics
Authors: An Hongbin , Chen Ruoyu , Li Saisai , Huang Lingfeng , Jia Wenbao , Wu Qianfang , Mao Aiqing 
Published: March 2024

AbstractPowder synthesis is crucial for achieving the densification and facilitating widespread applications of high entropy carbide (HEC). Specifically, the production of fine particle‐size powders is essential to enhance the densification of HECs. Consequently, this paper proposes the molten salt method for synthesizing HEC powders. The synthesis process successfully yielded (Ta Nb Ti V) C powders with a particle size of only 0.44 µm at 1400°C, exhibiting a uniform distribution of each element. Subsequent densification of (Ta Nb Ti V) C within the 1700–1900°C temperature range was carried out using spark plasma sintering. It was observed that as the densification temperature increased, the densification and mechanical properties of HEC significantly improved. This study presents innovative ideas for the preparation of HEC at lower temperatures.
Peak frequency mapping of atypical atrial flutter
Authors: Karatela Maham F. , Dowell Robert S. , Friedman Daniel J. , Jackson Kevin P. , Thomas Kevin L. , Piccini Jonathan P. 
Published: March 2024

AbstractIntroductionPeak frequency (PF) mapping is a novel method that may identify critical portions of myocardial substrate supporting reentry. The aim of this study was to describe and evaluate PF mapping combined with omnipolar voltage mapping in the identification of critical isthmuses of left atrial (LA) atypical flutters.Methods and ResultsLA omnipolar voltage and PF maps were generated in flutter using the Advisor HD‐Grid catheter (Abbott) and EnSite Precision Mapping System (Abbott) in 12 patients. Normal voltage was defined as ≥0.5 mV, low‐voltage as 0.1−0.5 mV, and scar as <0.1 mV. PF distributions were compared with ANOVA and post hoc Tukey analyses. The 1 cm radius from arrhythmia termination was compared to global myocardium with unpaired t‐testing. The mean age was 65.8 ± 9.7 years and 50% of patients were female. Overall, 34 312 points were analyzed. Atypical flutters most frequently involved the mitral isthmus (58%) or anterior wall (25%). Mean PF varied significantly by myocardial voltage: normal (335.5 ± 115.0 Hz), low (274.6 ± 144.0 Hz), and scar (71.6 ± 140.5 Hz) (p < .0001 for all pairwise comparisons). All termination sites resided in low‐voltage regions containing intermediate or high PF. Overall, mean voltage in the 1 cm radius from termination was significantly lower than the remaining myocardium (0.58 vs. 0.95 mV, p < .0001) and PF was significantly higher (326.4 vs. 245.1 Hz, p < .0001).ConclusionLow‐voltage, high‐PF areas may be critical targets during catheter ablation of atypical atrial flutter.
On the occurrence of a post‐larval specimen of Brosme brosme (Gadiformes: Lotidae) on Porcupine Bank (west Ireland)
Authors: Baldó Francisco , De Carlos Alejandro , Bañón Rafael 
Published: March 2024

AbstractThe occurrence of a small specimen of Brosme brosme (Gadiformes: Lotidae) from the Porcupine Bank is reported. A single specimen with a total length of 73.2 mm was caught with bottom trawl at a depth of 322 m depth in 2017. The specimen was identified morphologically and confirmed by molecular taxonomy using DNA barcoding. Based on the size and ontogenetic characters found, the specimen was identified as a post‐larval individual, and a pelagic habitat of the specimen seems more likely.
Neuroendocrine neoplasms: Consensus on a patient care pathway
Authors: Hooper Jessica , Jervis Nikie , Morgan Lucy , Beckett Vivienne , Hand Philippa , Higgs Kate , Munir Alia , Prinn Jenny , Pritchard D Mark , Sarker Debashis , Srirajaskanthan Raj , Ellis Catherine Bouvier 
Published: March 2024

AbstractPeople with neuroendocrine neoplasms (NENs) face a multitude of challenges, including delayed diagnosis, low awareness of the cancer among healthcare professionals and limited access to multidisciplinary care and expert centres. We have developed the first patient care pathway for people living with NENs in England to guide disease management and help overcome these barriers. The pathway was developed in two phases. First, a pragmatic review of the literature was conducted, which was used to develop a draft patient care pathway. Second, the draft pathway was then updated following semi‐structured interviews with carefully selected expert stakeholders. After each phase, the pathway was discussed among a multidisciplinary, expert advisory group (which comprised the authors and the Deputy Chief Operating Officer, West Suffolk NHS Foundation Trust), who reached a consensus on the ideal care pathway. This article presents the outputs of this research. The pathway identified key barriers to care and highlighted how these may be addressed, with many of the findings relevant to the rest of the UK and international audiences. NENs are increasing in incidence and prevalence in England, compounding pre‐existing inequities in diagnosis and disease management. Effective integration of this pathway within NHS England will help achieve optimal, equitable care provision for all people with NENs, and should be feasible within the existing expert multidisciplinary teams across the country.
Interventions to improve nurse–family communication in the emergency department: A scoping review
Authors: Bissonette Sarah , Chartrand Julie , Bailey Liana , Lalonde Michelle , Tyerman Jane 
Published: March 2024

AbstractAimTo determine and describe what interventions exist to improve nurse–family communication during the waiting period of an emergency department visit.BackgroundCommunication between nurses and families is an area needing improvement. Good communication can improve patient outcomes, satisfaction with care and decrease patient and family anxiety.DesignScoping Review.MethodsA scoping review was conducted following the Joanna Briggs Institution methodology: (1) identify the research question, (2) define the inclusion criteria, (3) use a search strategy to identify relevant studies using a three‐step approach, (4) select studies using a team approach, (5) data extraction, (6) data analysis, and (7) presentation of results.Data SourcesMedline, CINAHL, EMBASE, PsychInfo and grey literature were searched on 3 August 2022.ResultsThe search yielded 1771 articles from the databases, of which 20 were included. An additional seven articles were included from the grey literature. Paediatric and adult interventions were found targeting staff and family of which the general recommendations were summarised into communication models.ConclusionFuture research should focus on evaluating the effectiveness of interventions using a standardised scale, understanding the specific needs of families, and exploring the communication models developed in this review.Implications for Clinical PracticeCommunication models for triage nurses and all emergency department nurses were developed. These may guide nurses to improve their communication which will contribute to improving family satisfaction.Reporting MethodPRISMA‐ScR.Trial and Protocol RegistrationProtocol has been registered with the Open Science Framework, registration number 10.17605/OSF.IO/ETSYB.Patient or Public ContributionNo patient or public contribution.
Product categorization of Korean rice wine (makgeolli) compared to beer and wine using sensory evaluation methods
Authors: Wong Barry , Phillips Megan , Kam Rothman 
Published: March 2024

AbstractThis study aims to investigate the product categorization and characterization of makgeolli (Korean rice wine [KRW]) among beer and wine samples. Projective mapping with ultra‐flash profiling (PM with UFP) (n = 68) was conducted with 12 alcoholic beverage samples, including wines, beers, and makgeolli, and showed that participants characterized wine based on fruitiness, beer on bitterness, and makgeolli on sourness. s showed that PM with UFP had a combined explained variance of 47.4% with beer, wine, and makgeolli grouped separately. However, when participants were separated based on wine knowledge, low wine knowledge participants placed Shiraz close to makgeolli indicating participants were using a different metric in grouping the alcoholic beverage samples. When participants were tasked with using polarized sensory positioning (n = 91) to characterize makgeolli and wines, participants regardless of wine knowledge showed that wine and makgeolli had unique sensory traits and thus should be categorized differently.Practical ApplicationsResearch on KRW (makgeolli) is relatively limited in Western societies and this study attempts to understand how participants categorize makgeolli in New Zealand among different alcoholic beverages using projective and characterizing methods. Based on the categorization theory, it is important to position the product properly in the food and beverage categories as consumers may use the category cues to evaluate makgeolli and use products such as beer or wine within the same category as price anchors for makgeolli's price in the market. The results showed that participants categorize makgeolli differently compared to wine and beer; therefore, it would be beneficial to position makgeolli separately. Practical applications may include creating a separate section on a restaurant beverage menu, a webpage on an alcoholic beverage seller's website, or a small sub‐section on the retail store shelf. Placing makgeolli in the wine section as previously suggested is not recommended.
Picto‐verbal representation: Types and features of unreliable narration in comics
Authors: Xu Lian 
Published: March 2024

AbstractScholars have underestimated comics as an art form. This article uses an analysis of unreliable narration to show the complexity of this narrative mechanism in comics and, in turn, the sophistication of comics. Groensteen divides comic narrators into reciters and monstrators, and this distinction provides the basis to discuss unreliable narration in comics. This article categorizes comics with unreliable narration into mono‐sequence and dual‐sequence unreliable narrations according to the picto‐verbal relationship in the works. Moreover, medium‐specific features of comics such as spatialization of time and panel designs lead to unique types of unreliability.
Histological alterations in chilli pepper (Capsicum annuum L.) roots associated with sudden wilt syndrome
Authors: Salaria Pooja , Jain Sandeep , Kalia Anu , Anjali  
Published: March 2024

AbstractSudden wilt syndrome of chilli, an emerging and destructive disorder, is characterized by an abrupt appearance that has increased in Indo‐Gangetic alluvial plains over the past decade. The primary cause associated with the problem is water stagnation that creates hypoxic conditions in the root zone, and the plant mortality is further aggravated by soil‐borne fungi Fusarium oxysporum. The effects of sudden wilt on chilli roots were studied morphologically and histologically, using root scanning, optical microscopy and electron microscopy. Significant changes with respect to root length, projected area, number of root tips and root segments, average root diameter as well as estimated volume of roots were observed via root scanning of healthy and diseased root samples. Through optical microscopy of sections of the microtome‐cut root junctions displayed normal epidermis in healthy plants, while swollen cells indicated stress in the diseased plants. Cells of laterals and root tips in healthy plants were intact and stained strongly, but because of damaged tissues, cells in diseased plants were macerated and stained weakly. Root tips of healthy plants contained mitotic zones, whereas diseased root tips lacked mitotic zones. Electron microscopy studies revealed that sudden wilt had an adverse impact on xylem diameter, stele diameter, epidermal thickness and cortex thickness as evidenced by significantly lesser values of these parameters. The present study is the first systematic attempt to examine the morphological, histological and ultrastructural changes in chilli in response to sudden wilt syndrome.
Healthcare for people with intellectual disabilities in the Netherlands
Authors: Huisman Sylvia , Festen Dederieke , Bakker‐van Gijssel Esther 
Published: March 2024

AbstractIn this article, we describe the healthcare system for people with intellectual disabilities (ID) in the Netherlands. The general background about healthcare for people with ID is the same worldwide: their health needs are often unrecognized and unmet. We delineate, from a historical perspective, the steps the Netherlands has taken to change the situation for them. The Netherlands crossed a milestone in 2016 when it ratified the UN Convention on the Rights of Persons with Disabilities. Despite challenges in establishing numbers, an estimated ID prevalence of 1.45% was determined. The Dutch healthcare system has different levels, is funded through six distinct laws, and is complicated. The Netherlands has a spectrum of disability care services that increasingly collaborate in shaping the care of people with ID. People with ID and their representatives are increasingly involved in the process of shaping this care. The general practitioner plays a central role in the Dutch healthcare system, serving as the gatekeeper to medical specialists. Furthermore, the Netherlands recognizes the role of a physician for people with ID as a medical specialization. The core competencies of the ID physician include knowledge of the etiology and consequences of ID and associated health problems. The ID physician also knows how to deal with diagnostic and therapeutic barriers. Key challenges facing ID healthcare in the Netherlands include difficulties supporting people with ID due to the increasing complexity of society, concerns about continuity of care at the transition age (18−/18+), inadequate reach of population screening programs for people with ID, and limited availability of (routine) data for research on the ID population. The Dutch government encourages research in the ID field to overcome these challenges by financially supporting academic collaboratives. Substantial progress has been made, but key challenges remain, showing that there is still great room for improvement.
Comparison of chemotherapy outcomes between normal and high serum cortisol concentration in dogs with lymphoma
Authors: Yamazaki Hiroki , Bunbai Kaito , Deguchi Tatsuya , Tamura Masahiro , Ohota Hiroshi 
Published: March 2024

AbstractBackgroundIncreased serum cortisol (COR) concentrations may induce glucocorticoid resistance by down‐regulation of glucocorticoid receptor (GCR), resulting in decreased chemotherapy efficacy in dogs with lymphoma.HypothesisInvestigate the relationship between serum COR concentrations and chemotherapy outcomes in dogs with lymphoma.AnimalsThirty client‐owned dogs with lymphoma, with serum COR concentration measured using serum samples collected at diagnosis.MethodsRetrospective study. Dogs were divided into 2 groups based on serum COR concentrations: a normal group (n = 16) with COR concentrations <6 μg/dL and a high group (14) with COR concentrations ≥6 μg/dL. We compared signalment, clinical signs, stage, type of lymphoma, adrenal gland size, alkaline phosphatase (ALP) activity, response to chemotherapy, progression‐free survival (PFS), overall survival (OS), and rate of P‐glycoprotein (P‐gp)‐ and GCR‐positive cells between the 2 groups.ResultsNo significant differences were found in the demographic characteristics between the 2 groups. However, the high COR group exhibited a significantly lower response to chemotherapy, PFS, and OS compared with the normal COR group. Serum ALP activity was significantly higher in the high COR group than in the normal COR group. Adrenal gland size was also significantly larger in the high COR group. Although no significant differences were found in the rate of P‐gp‐positive cells between the 2 groups, the rate of GCR‐positive cells was significantly lower in the high COR group.Conclusions and Clinical ImportanceOur data suggests that measurement of serum COR concentrations may serve as a potential prognostic factor and evaluation index.