bluuebunny/crossref_metadata_2025_split huggingface dataset:
Parenting Practices Are Associated With Out-of-School Physical Activity in US Adolescents in 2014
Authors: Hiya Farhan , Lamour Jean-Paul M. , Khan Anwar A. , Wood Robert , Rodriguez de la Vega Pura E. , Castro Grettel , Ruiz Juan G. , Barengo Noel C. 
Published: April 2024

Introduction: Lack of physical activity (PA) is associated with obesity, diabetes, hypertension, cardiovascular diseases, and cancer. Parenting practices influence PA in young children. However, there is little evidence available for adolescents. We examined whether parenting practices were associated with out-of-school PA (OSPA) in US adolescents. Methods: This cross-sectional 2019 study analyzed data from the 2014 FLASHE study, a web-based, quota-sampled survey of parent–adolescent dyads. Inclusion required survey completion and parents to live with their teen (ages 12–17 y old). Physically limited adolescents were excluded. Dyads were stratified by teen age. Exposures included parental modeling, monitoring, facilitation, restriction, guided choice, and pressure. The outcomes of interest were OSPA Youth Activity Profile scores. Odds ratios (ORs) with 95% confidence intervals (CI) were calculated using adjusted logistic regressions. Results: A total of 1109 dyads were included. Guided choice increased odds of OSPA for 15- to 17-year-olds (OR = 2.12; 95% CI, 1.17–3.84). Facilitation increased odds of OSPA for 12- to 14-year-olds (OR = 2.21; 95% CI, 1.13–4.33). Monitoring decreased odds of OSPA for 15- to 17-year-olds (OR = 0.34; 95% CI, 0.20–0.57) and 12- to 14-year-olds (OR = 0.45; 95% CI, 0.27–0.74). Friend support increased odds of OSPA in 15- to 17-year-olds (OR = 4.03; 95% CI, 2.29–7.08) and 12- to 14-year-olds (OR = 3.05; 95% CI 1.69–5.51). Conclusion: Future interventions should prioritize (1) shared decision making for older teens, (2) access to PA opportunities for younger adolescents, and (3) promoting peer PA and friend support for everyone.
Establishment of replication-competent vesicular stomatitis virus recapitulating SADS-CoV entry
Authors: Zhu Zihui , Han Yutong , Gong Mingli , Sun Bo , Zhang Rong , Ding Qiang 
Published: April 2024

ABSTRACT

Zoonotic coronaviruses pose a continuous threat to human health, with newly identified bat-borne viruses like swine acute diarrhea syndrome coronavirus (SADS-CoV) causing high mortality in piglets.
In vitro
studies indicate that SADS-CoV can infect cell lines from diverse species, including humans, highlighting its potential risk to human health. However, the lack of tools to study viral entry, along with the absence of vaccines or antiviral therapies, perpetuates this threat. To address this, we engineered an infectious molecular clone of Vesicular Stomatitis Virus (VSV), replacing its native glycoprotein (G) with SADS-CoV spike (S) and inserting a Venus reporter at the 3′ leader region to generate a replication-competent rVSV-Venus-SADS S virus. Serial passages of rVSV-Venus-SADS S led to the identification of an 11-amino-acid truncation in the cytoplasmic tail of the S protein, which allowed more efficient viral propagation due to increased cell membrane anchoring of the S protein. The S protein was integrated into rVSV-Venus-SADS SΔ11 particles, susceptible to neutralization by sera from SADS-CoV S1 protein-immunized rabbits. Additionally, we found that TMPRSS2 promotes SADS-CoV spike-mediated cell entry. Furthermore, we assessed the serum-neutralizing ability of mice vaccinated with rVSV-Venus-SADS SΔ11 using a prime-boost immunization strategy, revealing effective neutralizing antibodies against SADS-CoV infection. In conclusion, we have developed a safe and practical tool for studying SADS-CoV entry and exploring the potential of a recombinant VSV-vectored SADS-CoV vaccine.

IMPORTANCE
Zoonotic coronaviruses, like swine acute diarrhea syndrome coronavirus (SADS-CoV), pose a continual threat to human and animal health. To combat this, we engineered a safe and efficient tool by modifying the Vesicular Stomatitis Virus (VSV), creating a replication-competent rVSV-Venus-SADS S virus. Through serial passages, we optimized the virus for enhanced membrane anchoring, a key factor in viral propagation. This modified virus, rVSV-Venus-SADS SΔ11, proved susceptible to neutralization, opening avenues for potential vaccines. Additionally, our study revealed the role of TMPRSS2 in SADS-CoV entry. Mice vaccinated with rVSV-Venus-SADS SΔ11 developed potent neutralizing antibodies against SADS-CoV. In conclusion, our work presents a secure and practical tool for studying SADS-CoV entry and explores the promise of a recombinant VSV-vectored SADS-CoV vaccine.
Improved empirical formula for spontaneous fission half-lives
Authors: Babu A. V. Mahesh , Dhananjaya N. , Manjunatha H. C. , Sowmya N. , Nagaraja A. M. 
Published: April 2024

The spontaneous fission (SF) half-lives of all 96 experimentally accessible SF emitters were examined. The SF emitters are classified as even Z-even A, odd Z-even A, odd Z-odd A and even Z-odd A. A plot of [Formula: see text] versus [Formula: see text] demonstrates a straight line, where k is variable. The [Formula: see text] values produced in this study are compared to all other semi-empirical relations such as Ren et al., [Nucl. Phys. A 759, 64 (2005)], Xu et al., [Phys. Rev. C 78, 044329 (2008)], Santhosh et al., [Nucl. Phys. A 832, 220 (2010)] and Karpov et al., [Int. J. Mod. Phys. E 21, 1250013 (2012)] available in the literature. When compared to earlier semi-empirical equations accessible in the literature, the new formula shows reduced uncertainty in standard deviation in the case of Odd Z-even A and even Z-Odd A nuclei. When compared to other sets of combinations (even Z-even A, and odd Z-odd A nuclei), this study more accurately reproduces the experimental SF half-lives for even Z-odd A and odd Z-even A nuclei with smaller [Formula: see text] in the atomic number range 9[Formula: see text]12 and mass number range 23[Formula: see text]84.
Supplementary Table S2 from Human Tumor–Associated Macrophages and Neutrophils Regulate Antitumor Antibody Efficacy through Lethal and Sublethal Trogocytosis
Authors: Singhal Sunil , Rao Abhishek S. , Stadanlick Jason , Bruns Kyle , Sullivan Neil T. , Bermudez Andres , Honig-Frand Adam , Krouse Ryan , Arambepola Sachinthani , Guo Emily , Moon Edmund K. , Georgiou George , Valerius Thomas , Albelda Steven M. , Eruslanov Evgeniy B. 
Published: April 2024

<p>Supplementary Table S2. Heatmap showing comparisons of the abilities of different types of blood and tumor FcR+ effectors to affect tumor cell growth depending on targeted tAg density, E:T ratios, and anti-tumor Ab isotypes. The indicated FcR+ effectors were added to EGFRhiA431 (high tAg density) or EGFRloA549 (low tAg density) tumor cells at different E:T ratios in the presence of cetuximab (IgG) or IgA anti-EGFR Ab (IgA). The effect on the tumor cell growth was determined at 48 hours using the IncuCyte Live Cell Analysis System. The numbers on the heatmap represent the percent of inhibition of tumor growth. A positive value indicates the inhibition of tumor growth (degrees of red color). A negative value indicates the stimulation of tumor cell growth (degrees of green color). The numbers represent the mean values obtained from experiments shown in Fig.1I, 1K, 4I, and 4J.</p>
Supplementary Figure S6 from Human Tumor–Associated Macrophages and Neutrophils Regulate Antitumor Antibody Efficacy through Lethal and Sublethal Trogocytosis
Authors: Singhal Sunil , Rao Abhishek S. , Stadanlick Jason , Bruns Kyle , Sullivan Neil T. , Bermudez Andres , Honig-Frand Adam , Krouse Ryan , Arambepola Sachinthani , Guo Emily , Moon Edmund K. , Georgiou George , Valerius Thomas , Albelda Steven M. , Eruslanov Evgeniy B. 
Published: April 2024

<p>Supplementary Figure S6. (A and B) Representative experiment and summary results showing the effect of indicated blood and tumor FcR+ effectors on the growth of A431 tumor cell spheroids in the presence of cetuximab (1ug/ml). GFP+A431 cells were seeded at 500 cells/well in a round bottom ultra-low adhesion cell culture plate designed to form spheroids. Indicated FcR+ effectors were added at E:T ratio 50:1 into the spheroid cultures. The growth of tumor spheroids was monitored in the IncuCyte® Live Cell Analysis System for 48 hrs. Representative images of GFP+A431 tumor cell spheroids co-cultured with indicated effectors and cetuximab for 48 hrs taken from IncuCyte® Live Cell Analysis System (original magnification x10). Scale bar, 400 µm. Percentage of tumor cell growth inhibition/stimulation in the presence of anti-EGFR Abs was calculated at 48 hrs using the formula: (FI)(A431+Ab)-FI(A431+effectors+Ab)/FI(A431+Ab)x100%, FI-Integrated fluorescence intensity. Data are represented as mean ± SEM. Wilcoxon matched pairs test (n=7). (C) Representative dotplots showing the different levels of ADT mediated by indicated FcR+ effectors co-cultured with spheroids formed with PKH67-labeled A431 cells. FcR+ effectors were co-cultured at a E:T ratio 50:1 in the presence or absence of cetuximab for 12 hrs. Representative results from 1 of 3 experiments are shown. (D and E) Representative experiment and summary results showing the effect of PBN and TAN on the growth of GFP+A431 tumor cell spheroids in the presence of IgA anti-EGFR Ab (IgA) (1ug/ml). Experiments were performed as described in (A and B). Original magnification x10. Scale bar, 400 µm. Data are represented as mean ± SEM. Wilcoxon matched pairs test (n=5). (F) Representative dotplots showing the ADT mediated by PBN and TAN co-cultured with spheroids formed with PKH67-lableld A431 cells. PBN and TAN were co-cultured at a E:T ratio 50:1 in the presence or absence of IgA anti-EGFR Ab for 12 hrs. Representative results from 1 of 3 experiments are shown.</p>
Simulated Warming Hole in Paleo-Pacific Oceans
Authors: Wei Mengyu , Yang Jun , Hu Yongyun , Liu Yonggang , Hu Shineng , Li Xiang , Lan Jiawenjing , Guo Jiaqi , Yuan Shuai , Nie Ji 
Published: April 2024

Abstract
Both observations and simulations show that under global warming, there exists a warming deficit in the North Atlantic, known as the North Atlantic warming hole (NAWH). Here, we show that a similar warming hole occurs in the subpolar Pacific Ocean of paleoclimate simulations. As the solar constant is increased, the local surface becomes substantially cooler rather than warmer in the subpolar paleo-Pacific Ocean under the land–sea configurations of 70, 90, and 150 million years ago (Ma). The warming hole has a magnitude of ≈3°C and is located in the Northern Hemisphere in 70 and 90 Ma. The warming hole in 150 Ma has a magnitude of ≈1°C and is located in the Southern Hemisphere. Both atmospheric and oceanic processes contribute to trigger the warming hole. For 70- and 90-Ma experiments, atmospheric teleconnection along a great circle from tropics to extratropics intensifies surface winds over subpolar ocean and thereby increases relatively cool seawater transport from high to low latitudes. Meanwhile, global meridional overturning circulation (GMOC) becomes weaker, causing a divergence of the meridional ocean heat transport in the warming hole region. An increasing regional cloud shortwave cooling effect acts to further enhance the warming hole. For 150-Ma experiments, the warming hole is related to the meridional shift of midlatitude jet stream and the weakening of GMOC in the Southern Hemisphere. The strength and phase of the atmospheric teleconnection and the response of GMOC strongly depend on land–sea configuration, resulting in the paleo-Pacific warming hole to occur in special periods only.
Chest Tube Size Selection: Evaluating Provider Practices, Treatment Efficacy, and Complications in Management of Thoracic Trauma
Authors: Messa Genevieve E. , Fontenot Cameron J. , Deville Paige E. , Hunt John P. , Marr Alan B. , Schoen Jonathan E. , Stuke Lance E. , Greiffenstein Patrick P. , Smith Alison A. 
Published: April 2024

Background The standard for managing traumatic pneumothorax (PTX), hemothorax (HTX), and hemopneumothorax (HPTX) has historically been large-bore (LB) chest tubes (>20-Fr). Previous studies have shown equal efficacy of small-bore (SB) chest tubes (≤19-Fr) in draining PTX and HTX/HPTX. This study aimed to evaluate provider practice patterns, treatment efficacy, and complications related to the selection of chest tube sizes for patients with thoracic trauma. Methods A retrospective chart review was performed on adult patients who underwent tube thoracostomy for traumatic PTX, HTX, or HPTX at a Level 1 Trauma Center from January 2016 to December 2021. Comparison was made between SB and LB thoracostomy tubes. The primary outcome was indication for chest tube placement based on injury pattern. Secondary outcomes included retained hemothorax, insertion-related complications, and duration of chest tube placement. Univariate and multivariate analyses were performed. Results Three hundred and forty-one patients were included and 297 (87.1%) received LB tubes. No significant differences were found between the groups concerning tube failure and insertion-related complications. LB tubes were more frequently placed in patients with penetrating MOI, higher average ISS, and higher average thoracic AIS. Patients who received LB chest tubes experienced a higher incidence of retained HTX. Discussion In patients with thoracic trauma, both SB and LB chest tubes may be used for treatment. SB tubes are typically placed in nonemergent situations, and there is apparent provider bias for LB tubes. A future randomized clinical trial is needed to provide additional data on the usage of SB tubes in emergent situations.
Increased CX3CL1 in cerebrospinal fluid and ictal serum t-tau elevations in migraine: results from a cross-sectional exploratory case-control study
Authors: Süße Marie , Kloetzer Christine , Strauß Sebastian , Ruhnau Johanna , Overeem Lucas Hendrik , Bendig Merle , Schulze Juliane , Reuter Uwe , Vogelgesang Antje , Fleischmann Robert 
Published: April 2024

Abstract
Background
To date, migraine is diagnosed exclusively based on clinical criteria, but fluid biomarkers are desirable to gain insight into pathophysiological processes and inform clinical management. We investigated the state-dependent profile of fluid biomarkers for neuroaxonal damage and microglial activation as two potentially relevant aspects in human migraine pathophysiology.

Methods
This exploratory study included serum and cerebrospinal fluid (CSF) samples of patients with migraine during the headache phase (ictally) (n = 23), between attacks (interictally) (n = 16), and age/sex-matched controls (n = 19). Total Tau (t-Tau) protein, glial fibrillary acidic protein (GFAP), ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), and neurofilament light chain (NfL) were measured with the Neurology 4-plex kit on a Single Molecule Array SR-X Analyzer (Simoa® SR-X, Quanterix Corp., Lexington, MA). Markers of microglial activation, C-X3-C motif chemokine ligand 1 (CX3CL1) and soluble triggering receptor expressed on myeloid cells 2 (sTREM2), were assessed using an immunoassay.

Results
Concentrations of CX3CL1 but not sTREM2 were significantly increased both ictally and interictally in CSF but not in serum in comparison to the control cohort (p = 0.039). ROC curve analysis provided an AUC of 0.699 (95% CI 0.563 to 0.813, p = 0.007). T-Tau in serum but not in CSF was significantly increased in samples from patients taken during the headache phase, but not interictally (effect size: η2 = 0.121, p = 0.038). ROC analysis of t-Tau protein in serum between ictal and interictal collected samples provided an AUC of 0.729 (95% CI 0.558 to 0.861, p = 0.006). The other determined biomarkers for axonal damage were not significantly different between the cohorts in either serum or CSF.

Discussion
CX3CL1 in CSF is a novel potential fluid biomarker of migraine that is unrelated to the headache status. Serum t-Tau is linked to the headache phase but not interictal migraine. These data need to be confirmed in a larger hypothesis-driven prospective study.
Developing an ensembled machine learning model for predicting water quality index in Johor River Basin
Authors: Sidek L. M. , Mohiyaden H. A. , Marufuzzaman M. , Noh N. S. M. , Heddam Salim , Ehteram Mohammad , Kisi Ozgur , Sammen Saad Sh. 
Published: April 2024

AbstractCurrently, the Water Quality Index (WQI) model becomes a widely used tool to evaluate surface water quality for agriculture, domestic and industrial. WQI is one of the simplest mathematical tools that can assist water operator in decision making in assessing the quality of water and it is widely used in the last years. The water quality analysis and prediction is conducted for Johor River Basin incorporating the upstream to downstream water quality monitoring station data of the river. In this research, the numerical method is first used to calculate the WQI and identify the classes for validating the prediction results. Then, two ensemble and optimized machine learning models including gradient boosting regression (GB) and random forest regression (RF) are employed to predict the WQI. The study area selected is the Johor River basin located in Johor, Peninsular Malaysia. The initial phase of this study involves analyzing all available data on parameters concerning the river, aiming to gain a comprehensive understanding of the overall water quality within the river basin. Through temporal analysis, it was determined that Mg, E. coli, SS, and DS emerge as critical factors affecting water quality in this river basin. Then, in terms of WQI calculation, feature importance method is used to identify the most important parameters that can be used to predict the WQI. Finally, an ensemble-based machine learning model is designed to predict the WQI using three parameters. Two ensemble ML approaches are chosen to predict the WQI in the study area and achieved a R2 of 0.86 for RF-based regression and 0.85 for GB-based ML technique. Finally, this research proves that using only the biochemical oxygen demand (BOD), the chemical oxygen demand (COD) and percentage of dissolved oxygen (DO%), the WQI can be predicted accurately and almost 96 times out of 100 sample, the water class can be predicted using GB ensembled ML algorithm. Moving forward, stakeholders may opt to integrate this research into their analyses, potentially yielding economic reliability and time savings.
Physiological and transcriptomic comparisons shed light on the cold stress response mechanisms of Dendrobium spp
Authors: Li Zhiyuan , Lu Shunjiao , Yi Shuangshuang , Mo Shunjin , Yu Xiaoyun , Yin Junmei , Zhang Changqing 
Published: April 2024

Abstract
Background
Dendrobium spp. comprise a group of tropical orchids with ornamental and medicinal value. Dendrobium spp. are sensitive to low temperature, and the underlying cold response regulatory mechanisms in this group are unclear. To understand how these plants respond to cold stress, we compared the transcriptomic responses of the cold-tolerant cultivar ‘Hongxing’ (HX) and the cold-sensitive cultivar ‘Sonia Hiasakul’ (SH) to cold stress.

Results
Chemometric results showed that the physiological response of SH in the later stages of cold stress is similar to that of HX throughout the cold treatment. Orthogonal partial least squares discriminant analysis (OPLS–DA) revealed that soluble protein content and peroxidase activity are key physiological parameters for assessing the cold tolerance of these two Dendrobium spp. cultivars. Additionally, weighted gene co-expression network analysis (WGCNA) results showed that many cold response genes and metabolic pathways significantly associated with the physiological indices were enriched in the 12 detected modules. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) enrichment analyses of the 105 hub genes showed that Dendrobium spp. adapt to cold stress by regulating signal transduction, phytohormones, transcription factors, protein translation and modification, functional proteins, biosynthesis and metabolism, cell structure, light, and the circadian clock. Hub genes of the cold stress response network included the remorin gene pp34, the abscisic acid signaling pathway-related genes PROTEIN PHOSPATASE 2 C (PP2C), SNF1-RELATED PROTEIN KINASE 2 (SnRK2), ABRE-BINDING FACTOR 1 (ABF1) and SKI-INTERACTING PROTEIN 17 (SKIP17), the Ca2+ signaling-related GTP diphosphokinase gene CRSH1, the carbohydrate-related gene STARCH SYNTHASE 2 (SS2), the cell wall biosynthesis gene CINNAMYL ALCOHOL DEHYDROGENASE (CAD7), and the endocytosis-related gene VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 52 A (VPS52A).

Conclusions
The cold-responsive genes and metabolic pathways of Dendrobium spp. revealed in this study provide important insight to enable the genetic enhancement of cold tolerance in Dendrobium spp., and to facilitate cold tolerance breeding in related plants.
Insight into the phylogeny and responses of species from the genus Sergia (Campanulaceae) to the climate changes predicted for the Mountains of Central Asia (a world biodiversity hotspot)
Authors: Vintsek Lizaveta , Klichowska Ewelina , Nowak Arkadiusz , Nobis Marcin 
Published: April 2024

Abstract
Background
Together with other elevated areas, the Mountains of Central Asia are significantly threatened by ongoing climate change. The presence of refuges during the glaciations makes the region extremely rich in species, especially endemic ones. However, the limited potential for colonisation of other habitats makes rocky-related species with ‘island‐like’ distribution, particularly vulnerable to climate change. To understand the processes underlying species response to climate warming, we assessed differences in ecological niches and phylogenetic relationship of two geographically disjunctive alpine species belonging to the genus Sergia. The taxa are considered Tertiary relicts, endemic to the Tian Shan and Pamir-Alai Mountains. To illustrate range dynamics and differences in occupied niches of Sergia species, we used Ecological Niche Modelling of current and future distribution. Whereas, to reconstruct the phylogenetic relationship within and between Sergia and other related Campanulaceae species from the region we used molecular data (ITS, cpDNA, DArTseq-derived SNPs).

Results
The results reveal that the genus Sergia is a polyphyletic group, and its representatives differ geographically, ecologically and genetically. Both S. regelii and S. sewerzowii constitute a common clade with Asyneuma group, however, S. sewerzowii is more closely related to Campanula alberti (a species that has never previously been considered closely related to the genus Asyneuma or Sergia) than to S. regelii. Sergia sewerzowii is adapted to lower elevations with higher temperatures, while S. regelii prefers higher elevations with lower temperatures. The future distribution models demonstrate a dramatic loss of S. regelii range with a shift to suitable habitats in higher elevations, while the potential range of S. sewerzowii increases and shifts to the north.

Conclusions
This study shows that S. regelii and S. sewerzowii have a long and independent evolution history. Sergia regelii and S. sewerzowii significantly differ in realised niches. These differences are mirrored in the response of the studied endemics to future climate warming. As suitable habitats shrink, rapid changes in distribution can lead to species' range loss, which is also directly related to declines in genetic variability. The outcomes of this paper will help to more precisely assess the impact of climate changes on rocky-related plant species found in this world’s biodiversity hotspot.