bluuebunny/crossref_metadata_2025_split huggingface dataset:
Green synthesis of silver nanoparticles using Carob leaf extract: Characterization and analysing of toxic effects in model organism Galleria mellonella L. (The Greater Wax Moth)
Authors: Andırın Aslıhan , Yaycı Nur Dudu , Idikut Murat , Kara Ayse , Tuncsoy Mustafa , Tuncsoy Benay , Ozalp Pınar 
Published: April 2024

Abstract

Silver nanoparticles (AgNP) have been used in many studies due to their inhibitory properties on microorganisms such as bacteria and viruses. In recent years, due to global problems such as environmental pollution, the green synthesis (biosynthesis) method is frequently preferred because it is simple and low cost and does not require the use of toxic substances. In this study, it was determined that the effects on antioxidant enzyme activities (SOD, CAT, GPx, GST), acetylcholinesterase (AChE), and total hemocyte count (THC) as well as phenoloxidase activity to determine their effect on antioxidant defence and the immune system in model organism Galleria mellonella larvae. We observed that green synthesized AgNPs accumulate in the midgut of the larvae and led to the increasing of CAT and SOD activities. GST and AChE activities were increased in the fat body of the larvae otherwise; it was decreased in the midgut. Moreover, increases were found in THC and phenoloxidase activity. Consequently, green synthesized silver nanoparticles led to oxidative stress and immuntoxic effects on G. mellonella larvae.
Mindfulness training reduces the preference for sustainable outcomes
Authors: Corbi Zarah Le Houcq , Koch Kathrin , Hölzel Britta , Soutschek Alexander 
Published: April 2024

Abstract

Theoretical accounts posit that mindfulness promotes pro-environmental behavior. While this claim is supported by correlational findings, past intervention studies provided no evidence that enhancing mindfulness increases self-report measures of pro-environmental attitudes or behavior. Here, we tested whether a 31-day mindfulness intervention strengthens preferences for pro-environmental outcomes with decision tasks involving real conflict between participants’ selfish interests and beneficial consequences for the environment. To unravel the psychological mechanisms underlying the impact of mindfulness on sustainability, we assessed the impact of mindfulness training on prosociality and future orientation. Contrary to our hypotheses, the mindfulness intervention reduced instead of increased preferences for pro-environmental and prosocial outcomes, whereas no effects were observed on future orientation. Baseline preferences for pro-environmental and prosocial outcomes (and the intervention effects on them) were correlated, providing empirical evidence for a link between sustainability and prosociality. Together, the current data suggest that the relationship between mindfulness and sustainability as well as prosociality may be more complicated than assumed in the literature.
Regeneration of capacity-degraded LixNi0.6Co0.2Mn0.2O2（0 &lt; x &lt; 1）by molten salt method
Authors: Huang Li , Huang Junjie , Lian Hongxia , Wu Yiyun , Wang Zhifu , Wang Hao , Deng Heming , Cheng Qi 
Published: April 2024

Abstract

This paper provides a method for recycling spent lithium-ion battery cathode material NCM622, which is extremely innovative and simple to implement. The capacity of degraded NCM622 can be restored to the initial capacity by relithiation the degraded NCM622 with LiNO3-LiOH as the lithium salt. X-ray diffraction (XRD), inductively coupled plasma spectrometry (ICP), and scanning electron microscopy (SEM) are adopted to evaluate the properties of the regenerated NCM622. The capacity-degraded cathode particles with significant Li loss (≈20%) and capacity degradation (≈26.3%) can be successfully regenerated to achieve their original composition and crystal structures, leading to effective recovery of their capacity, cycling stability, and rate capability to the levels of the pristine materials. the first discharge capacity of pristine, capacity-degraded, regenerated was 172.5 mAh/g, 127.1 mAh/g, 170.5 mAh/g (0.1 C) . After cycling for 100 cycles at 1 C, the discharge capacity of all samples was 130. 5 mAh/g, 38.0 mAh/g, 133.1 mAh/g. This method can be widely used to recycle and regenerate NCM cathodes on a large scale of recycling from industrial production of LIBs.
Unveiling the Brazilian Kefir Microbiome: Discovery of a novel Lactobacillus kefiranofaciens (LkefirU) genome and in silico prospection of bioactive peptides with potential anti-Alzheimer properties
Authors: Silva Matheus Henrique , Batista Letícia Leandro , Malta Serena Mares , Santos Ana Carolina Costa , Mendes-Silva Ana Paula , Bonetti Ana Maria , Ueira-Vieira Carlos , Santos Anderson Rodrigues 
Published: April 2024

Abstract

Background- Kefir is a complex microbial community that plays a critical role in the fermentation and production of bioactive peptides, and has health-improving properties. In this study, we employed shotgun metagenomics and peptidomics approaches to further characterize Brazilian kefir.
Results- We successfully assembled the novel genome of Lactobacillus kefiranofaciens (LkefirU) and conducted a comprehensive pangenome analysis to compare it with other strains. Furthermore, we performed a peptidome analysis, revealing the presence of bioactive peptides encrypted by L. kefiranofaciens in the Brazilian kefir sample, and utilized in silicoprospecting and molecular docking techniques to identify potential anti-Alzheimer peptides, targeting β-amyloid (fibril and plaque), BACE, and acetylcholinesterase. Through this analysis, we identified two peptides that show promise as compounds with anti-Alzheimer properties.
Conclusions -These findings not only provide insights into the genome of L. kefiranofaciens but also serve as a promising prototype for the development of novel anti-Alzheimer compounds derived from Brazilian kefir.
Insights into Water Quality of River Yamuna, India: A Comprehensive Spatial-Temporal Analysis Using Advanced Indices and Multivariate Statistical Techniques.
Authors: Antil Sandeep , Maurya Swati , Dagar Jyoti , Bhagat Pooja , Makhija Seema , Toteja Ravi 
Published: April 2024

Abstract
The escalating issue of heavy metal pollution is a global concern, primarily due to its toxic and persistent nature, further exacerbated by a continuous surge in concentrations above recommended thresholds. This study examines the spatial-temporal dynamics of water quality along the Yamuna River through physicochemical parameters, heavy metal analysis, advanced approaches such as the Heavy Metal Pollution Index (HPI) & Ecological Risk Index (ERI), and multivariate statistical techniques (Principal Component Analysis). It was identified that Wazirabad after drain (WBAD) exhibits poor water quality, with levels of Dissolved Oxygen (DO), Ammonia, Electrical Conductivity (EC), and five heavy metals (Fe, Mn, Pb, Cr, and Ni) exceeding Bureau of Indian Standards (BIS) permissible limits. During the pre-monsoon season, HPI values surpassed the critical threshold (100) in WBBD (146.69), WBAD (267.13), and PJ (204.80), while in the post-monsoon season, only PJ (115) exhibited elevated HPI values. Ecological risk assessment highlighted Cd, Pb, and Ni as major risk elements, posing significant environmental threats. Pearson Correlation analysis and PCA identified two distinct groups of heavy metals, suggesting distinct contamination sources for Co, Cd, and Cu compared to the Fe-Cr-Pb-Mn-Zn-Ni group. This thorough study not only emphasizes the critical condition of water quality in the Delhi region but also provides valuable insights into the sources and distribution of heavy metals, offering a valuable foundation for targeted intervention and mitigation strategies to address the pressing issue of heavy metal pollution.
Reverse obliquity femoral neck fracture: two cases report and review of the literature
Authors: Huang Zheyuan , Fu Hongjun , Chen Xiaolin , Liu Haoyuan 
Published: April 2024

Abstract

Background:The literature defines a femoral neck fracture with a Pauwels angle of more than 70° as a vertical femoral neck fracture, which is one of the refractory fracture types. Clinically, there is a class of femoral neck fractures with a Pauwels angle ≥ 90° and a fracture line alignment that does not allow for Gotfried-positive support. we classified and named it as reverse obliquity femoral neck fracture（ROFNF）and report the treatment of two cases.
Case presentation: The first case was a 56-year-old female admitted with a right femoral neck fracture (Pauwels III-type, Pauwels angle 95°) caused by a slip and fall while playing table tennis. The second case was a 45-year-old male admitted with a right femoral neck fracture (Pauwels III-type, Pauwels angle 90°) due to a rollover accident while driving an electric car. The patients were supine on a fluoroscopic operating table and offered lumbar epidural anesthesia. After failure of closed anatomical repositioning under fluoroscopy, both patients were treated with incisional repositioning via an anterior hip approach and internal fixation with three 7.3 mm cannulated screws in combination with a medial femoral neck buttressplate. The follow‑up radiographs at 9 months showed complete union of the bone fractures.
Conclusions: This is the first reported of reverse obliquity femoral neck fracture with Pauwels angle≥ 90°. The course of treatment in our case showed that closed reduction and firm fixation of this rare type of femoral neck fracture were difficult, and open reduction via anterior hip approach and combined fixation were the optional treatment.
Transmutation of Palladium at Electrochemical Interfaces: Experimental and Theoretical Validation with Second Order Perturbation Theory
Authors: Gadly Trilochan , Phapale Suhas , Gamre Sunita , Jain Pankaj , Kumar Harishyam , Poudel Sanjeev , Desai Shraddha , Pathak Arup Kumar , Srivast Ashutosh , Kumar Amit , Pal Rupali , Chandwadkar Pallavi , Acharya Celin , Bakshi A. K. , Varma Salil , Patro Birija S , Goswami Dibakar 
Published: April 2024

Abstract

Electrolysis of D2O may be used as a portable neutron source with numerous applications without the complexity of huge reactor operations. Herein, we report reproducible fast neutron generation by electrolysis of D2O using palladium cathode and platinum anode, which was detected with diamond detector, gas filled 3He detectors after thermalisation with high density polythene, as well as novel epoxy resin and CR-39 detectors. Notably, a highly reproducible neutron generation at electrochemical surfaces of palladium electrode was observed and signature transmutation via Pd (d, n) Ag was corroborated. This was further explained using a theoretical model based on second order quantum perturbation theory.
Identification and validation of an inflammatory response-related signature for predicting prognosis and immunotherapeutic response in non-small cell lung cancer
Authors: Hu Xuelei , Jiang Tengfei , Wang Jinxiang 
Published: April 2024

Abstract

Background: Immunotherapy has revolutionized non-small cell lung cancer (NSCLC
) therapy, only a small proportion of patients demonstrate durable antitumor response due to the heterogeneity. Emerging evidence has linked inflammatory response to progression, anticancer therapeutic resistance, and poor prognosis of NSCLC. This study aimed to classify distinct inflammatory response-related subtypes and constructed a new risk model to predict the prognosis and guide immunotherapeutic treatments.
Methods:
The gene expression, somatic mutation and clinical parameters for patients with NSCLC were obtained from TCGA-LUAD, and were used as the training dataset. GSE41721 and GSE54467, from GEO database including gene expression and clinical parameters were used as external validation datasets. We distinguished the patients of TCGA-LUAD into three clusters by Consensus clustering analysis based on the “ConsensusClusterPlus” package. It was validated through clinical features, prognosis, tumor microenvironment, expression of immune checkpoints and somatic mutation profile in distinct inflammation-associated subtypes. The risk model was construct by the hub-gene, which were screened out from the inflammation-related genes by univariate Cox and LASSO Cox regression, and verified by time-dependent ROC and Kaplan–Meier analyses. In addition, IC50 was implemented with “oncoPredict” package and GDSC datasets to evaluate the difference of drug susceptibility, the immunotherapy response were investigated by the IMVigor210 datasets. Finally, the single cell RNA seq analysis was preformed to validate of inflammation genes expression pattern.
Results:
Our findings demonstrated that NSCLC can be devided into three subtypes by inflammatory response-related signature, namely, inflammation-low, inflammation-mid, and inflammation-high, each exhibiting distinct clinicopathological characteristics, prognostic implications, somatic mutation profile and tumor microenvironments. We have affirmed the reproducibility and predictability of this categorization. The inflammation-high subtype generally represents a poor prognosis characterized by high immune cell infiltration, high immune score, low tumor purity high expression of immune checkpoints and a high frequency of oncogene mutations. Conversely, the inflammation-low subtype exhibit favorable clinical outcomes, low immune cell infiltration, low immune score, high tumor purity low expression of immune checkpoints and a low frequency of oncogene mutations. Furthermore, we have developed an inflammatory response-related risk model that demonstrates robust efficiency in assessing prognosis, drug sensitivity and immunotherapy response.
Conclusions: In conclusion, we devided NSCLC into three subtypes and constructed a risk model based on the inflammatory response . This model was highly effective in predict the prognosis, as well as the immunotherapy response.
A heterogenous-source Geoinformation System to manage landscape modifications from a climate-change resilience perspective
Authors: Bellini Lorenzo Serra , Spanò Antonia , Bellini Marcello Cittadini , Tonolo Fabio Giulio 
Published: April 2024

Abstract

Historical landscapes in Italy have been changing slowly over the centuries since their features, once fixed in certain shapes, were perpetuated until new economic and social developments occurred. Yet, in the Alpine region, this territorial organization underwent sudden changes after WWII, resulting in a loss of population and traditional agropastoral production in favour of skiing plants and holiday houses. Moreover, the loss of traditional knowledge of environmental behaviour has resulted in urbanization of lands vulnerable to extreme events.
Nowadays, modern mapping technologies enable the assessment of the state of the landscape before, during, and after extreme events whose increased frequency is possibly related to climate change. The case study presented in the paper is the flood that hit Limone Piemonte, Italy, between October 2 and 3, 2020. On that occasion, an aerial survey of the affected areas was carried out by means of Uncrewed Aerial Vehicles (UAV) a few weeks after the event. Spatial analyses based on very high-resolution satellite imagery acquired a few days after the event were also carried out to integrate and support where to plan more detailed 3D surveys, enabling the assessment of damages at different map scales. Thanks to the availability of pre-event multi-temporal cartographic reference datasets it was possible to monitor the historical evolution of built-up area extent in the affected areas. It was possible to assess the vulnerable areas before the event and to evaluate the morphological and settlement changes after the disaster. Thus, we advocate that geoinformation systems are one of the main tools for studying environmental and climate alterations impacting landscapes. The ultimate goal was not only to produce a 3D model of the affected areas but also to provide the public administration with a sustainable and innovative tool for territorial and landscape management, in accordance with the 11th pillar of UN Sustainable Development Goals.
Exploration of Chemical Composition and Properties of Ancient Silver Mine Slag
Authors: KÜLEKÇİ Gökhan 
Published: April 2024

Abstract

This study focuses on the investigation of pozzolanic properties of the by-products generated from silver distillation processes in ancient times. The residues resulting from silver distillation are commonly described as slag or residues, representing unwanted materials left behind after metallurgical processes. However, these residues are now recognized as potential secondary raw material sources that can be economically utilized through secondary processes. Various analyses were conducted in this study to determine the pozzolanic properties of ancient mine slag. The methodology employed in the study involved grinding the mine residues to cement size and determining strength activity indexes with different pozzolan-cement mixtures. The obtained data indicates the potential pozzolanic activity of ancient mine slag. These findings provide significant implications for waste management and economic recycling. The pozzolanic properties of residues from silver distillation processes in ancient times serve as an important reference point for future research and industrial applications. This study contributes to the evaluation of residues carrying traces of metallurgical processes from ancient times through modern scientific methods, elucidating their potential value. Exploration of Pozzolanic Properties of Ancient Silver Distillation By-Products serves as a bridge spanning from ancient to modern times, offering important insights into how residues from prehistoric periods can be utilized in contemporary industrial processes."
Fine structure of a daytime mid-latitude sporadic-E revealed by GNSS TEC, InSAR, and MAI
Authors: Fujimoto Tatsuya , Furuya Masato , Heki Kosuke 
Published: April 2024

Abstract

Combining the total electron content (TEC) data from two nationwide Global Navigation Satellite System (GNSS) networks in Japan with the L-band synthetic aperture radar (SAR) data, we reveal the fine spatial and temporal structure of a daytime sporadic-E (Es) episode in Shikoku, Japan. The snapshot of the Es is derived not only from interferometric SAR (InSAR) but also from multiple aperture interferometry (MAI). The Es episode is accompanied by east-west elongated (up to ~ 180 km) multiple southward migrating TEC striations with a speed of ~ 90 m/s and ~ 10–20 km widths in the north-south direction. As previously suggested by the GNSS TEC time series, the present InSAR and MAI data independently confirm that electron density gradually increases from the frontal leading edge but abruptly drops in the trailing edge. The asymmetric electron density distribution is consistent with a previous study but requires further clarifications that can account for the occurrence in the daytime. The multiple TEC striations are reminiscent of the quasi-periodic (QP) echoes in nighttime Es detected by the Middle and Upper Atmosphere (MU) radar. Still, no vertically extended anomalies are suggested in the present daytime Es. The Kelvin-Helmholtz instabilities around the wind shear of neutral winds could be responsible for the QP TEC striations.
Coronary angiographic findings and clinical characteristics in patients with ST-elevation myocardial infarction: A cross-sectional study
Authors: Jand Abolfazl , amirfarhangi Abdollah , Sezavar seyed hashem , Naderi Maryam , eshrati Babak 
Published: April 2024

Abstract

Background: Coronary artery disease (CAD) is one of the most common causes of mortality worldwide. Several studies have demonstrated that some areas of the coronary arteries are more prone to thrombosis. This study investigated the associations between angiographic findings and risk factors for coronary artery disease.
Methods: This cross-sectional study investigated the total number of patients with ST-elevation myocardial infarction (STEMI) referred to the angiography department at Hazrat-E-Rasool Hospital from 21st March 2017 to 20th March 2018. All patients underwent standard coronary angiography; if necessary, a stent was deployed in the infarct-related artery (IRA). The referrals were made through emergency medical services (EMS) or the hospital's emergency department.
Results: Fifty-two patients were enrolled as STEMI patients and underwent coronary angiography at Hazrat-E-Rasool Hospital in 1396. The mean age of the patients was 59.7±12.7 years, and 78.9% were males. The most frequently involved vessels were the left anterior descending (LAD), right coronary artery (RCA), and left circumflex(LCX) Most lesions in the LAD were located in the mid-segment, while in the RCA and LCX, the involvement predominantly occurred in the proximal segment.
Conclusions: The results of our study demonstrated that the LAD artery is the most commonly involved vessel. Additionally, male sex, family history, and smoking are considered risk factors at a younger age, while diabetes, hypertension, and chronic kidney disease (CKD) play significant roles in older generations.
Plant species identity drives soil legacies by affecting root morphology of maize but effects depend on phosphorus supply
Authors: He Yujuan , Du Mulin , Yang Chuanli , Bi Yixian , Liu Hao , Struik Paul C. , Jing Jingying , Zhang Yingjun 
Published: April 2024

Abstract

Aims
Soil biotic legacies of leys in grassland-crop rotation systems affect growth of subsequent crops and are garnering increasing attention. We studied how different grass and legume species grown in monoculture or mixture affect the growth of the subsequent maize crop and whether the legacy effects depend on external phosphorus inputs.
Methods
We collected microbial inoculum from a five-year experiment with nine leys, including monocultures of two grasses and two legumes, and five legume-grass mixtures in different ratios (3:7, 4:6, 5:5, 6:4, 7:3), and conducted a greenhouse experiment to explore the biotic legacy effects conditioned by these nine leys on maize growth at three P levels (0, 20, 50 mg kg− 1).
Results
Significant biotic legacies on biomass of maize were only observed at high P. The biotic legacies and P application interactively impacted root morphology. Maize growth in soil conditioned by alfalfa monoculture showed stronger legacy effects by enriching beneficial bacteria and reducing accumulation of pathogenic fungi than maize growth in soil from the orchard grass monoculture. However, the effects of other monocultures and mixtures did not differ significantly.
Conclusion
Legacy effects on plant growth were proven to be ley-specific and depended on P input. These findings stress that biotic legacy effects interacted with external nutrient input have a significant role in determining the performance of subsequent crops.