bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot intervention to enhance sexual rehabilitation for couples after treatment for localized prostate carcinoma
Authors: Canada Andrea L. , Neese Leah E. , Sui Dawen , Schover Leslie R. 
Published: November 2005

AbstractBACKGROUNDThe majority of prostate carcinoma survivors experience enduring sexual difficulties and associated distress in the years after definitive treatment. A counseling intervention aimed at improving levels of sexual satisfaction and increasing successful utilization of medical treatment for erectile dysfunction (ED) was developed and pilot‐tested for both the survivor of prostate carcinoma and his partner.METHODSAll male participants were 3‐month to 5‐year survivors of localized prostate carcinoma who had been treated with radical prostatectomy or radiation therapy, and were married or in a committed relationship. Couples were randomized to attend four sessions of counseling together or to have the man attend alone. In both groups, partners completed behavioral homework. The sessions included education on prostate carcinoma and sexual function and options to treat ED as well as sexual communication and stimulation skills. Standardized questionnaires at baseline, posttreatment, and at 3‐month and 6‐month follow‐up assessed sexual function, marital adjustment, psychologic distress, and utilization of treatments for ED.RESULTSFifty‐one of 84 couples randomized to treatment completed the intervention (61%). Attendance by the partner did not affect outcomes. Participants completing the intervention demonstrated improvment in male overall distress (P < 0.01), male global sexual function (P < 0.0001), and female global sexual function (P < 0.05) at 3‐month follow‐up, but regression toward baseline was noted at 6‐month follow‐up. However, utilization of ED treatments increased from 31% at the time of study entry to 49% at the 6‐month follow‐up (P = 0.003).CONCLUSIONSThe results of this brief pilot counseling intervention demonstrated significant gains in sexual function and satisfaction and increased utilization of treatments for ED. However, modifications are needed in future randomized trials to reduce the rate of premature termination and to improve long‐term maintenance of gains. Cancer 2005. © 2005 American Cancer Society.
Distinct expression of TRPM8, TRPA1, and TRPV1 mRNAs in rat primary afferent neurons with aδ/c‐fibers and colocalization with trk receptors
Authors: Kobayashi Kimiko , Fukuoka Tetsuo , Obata Koichi , Yamanaka Hiroki , Dai Yi , Tokunaga Atsushi , Noguchi Koichi 
Published: November 2005

AbstractThe transient receptor potential (TRP) superfamily of cation channels contains four temperature‐sensitive channels, named TRPV1–4, that are activated by heat stimuli from warm to that in the noxious range. Recently, two other members of this superfamily, TRPA1 and TRPM8, have been cloned and characterized as possible candidates for cold transducers in primary afferent neurons. Using in situ hybridization histochemistry and immunohistochemistry, we characterized the precise distribution of TRPA1, TRPM8, and TRPV1 mRNAs in the rat dorsal root ganglion (DRG) and trigeminal ganglion (TG) neurons. In the DRG, TRPM8 mRNA was not expressed in the TRPV1‐expressing neuronal population, whereas TRPA1 mRNA was only seen in some neurons in this population. Both A‐fiber and C‐fiber neurons expressed TRPM8, whereas TRPV1 was almost exclusively seen in C‐fiber neurons. All TRPM8‐expressing neurons also expressed TrkA, whereas the expression of TRPV1 and TRPA1 was independent of TrkA expression. None of these three TRP channels were coexpressed with TrkB or TrkC. The TRPM8‐expressing neurons were more abundant in the TG compared with the DRG, especially in the mandibular nerve region innervating the tongue. Our data suggest heterogeneity of TRPM8 and TRPA1 expression by subpopulations of primary afferent neurons, which may result in the difference of cold‐sensitive primary afferent neurons in sensitivity to chemicals such as menthol and capsaicin and nerve growth factor. J. Comp. Neurol. 493:596–606, 2005. © 2005 Wiley‐Liss, Inc.
Inherent Complexities of Trace Detection by Surface‐Enhanced Raman Scattering
Authors: Pieczonka Nicholas P. W. , Aroca Ricardo F. 
Published: November 2005

AbstractSurface‐enhanced Raman scattering (SERS) and surface‐enhanced resonance Raman scattering (SERRS) are powerful optical scattering techniques used in such frontier areas of research as ultrasensitive chemical analysis, the characterization of nanostructures, and the detection of single molecules. However, measuring and, most importantly, interpreting SERS/SERRS spectra can be incredibly challenging. This is the result of modifications to the measured spectra that are due to of a variety of instabilities and contributions. These interferences and modifications arise from the nature of the enhancement itself, as well as the conditions used to attain SERS spectra. The present report is an attempt to collect in one place the analytical interferences that are most commonly found during the collection of SERS/SERRS spectra.
Aromaticity Analysis of Lithium Cation/ π Complexes of Aromatic Systems
Authors: Güell Mireia , Poater Jordi , Luis Josep M. , Mó Otilia , Yáñez Manuel , Solà Miquel 
Published: November 2005

AbstractThe changes in the local aromaticity of aromatic rings on complexation with lithium cation are discussed for a series of polycyclic aromatic hydrocarbons by using, as probes of aromaticity, thepara‐delocalization index (PDI), the aromatic fluctuation index (FLU), the harmonic oscillator model of aromaticity index (HOMA), and the nucleus‐independent chemical shift (NICS). In most cases, changes in the electronically based PDI and FLU indices are almost irrelevant. For this reason, the analysis is carried out mainly with the more sensitive HOMA and NICS descriptors. These indices indicate that changes in the local aromatic character of the different rings are small. In general, Li+interacts more favorably with the ring having the largest π charge, but there is no correlation between these magnitudes. The ring directly interacting with the lithium cation suffers a slight but significant reduction of aromaticity, while the reduction of the local aromaticity of the adjacent rings is minor. For those rings located further away from the ring directly connected to Li+, we found generally a small increase in aromaticity. Although there is no clear correlation between aromaticity and Li+binding enthalpies, there is a rough correlation between the latter and the change in the aromaticity of the ring directly interacting with the metal cation.
Probing the Water Coordination of Protein‐Targeted MRI Contrast Agents by Pulsed ENDOR Spectroscopy
Authors: Zech Stephan G. , Sun Wei‐Chuan , Jacques Vincent , Caravan Peter , Astashkin Andrei V. , Raitsimring Arnold M. 
Published: November 2005

AbstractA novel methodology based on electron–nuclear double resonance (ENDOR) spectroscopy is used for the direct determination of the water coordination number (q) of gadolinium‐based magnetic resonance imaging (MRI) contrast agents. Proton ENDOR spectra can be obtained at approximately physiological concentrations for metal complexes in frozen aqueous solutions either in the presence or absence of protein targets. It is shown that, depending on the structure of the co‐ligand, the water hydration number of a complex in aqueous solution can be significantly different to when the complex is noncovalently bound to a protein. From the ENDOR spectra of the exchangeable protons, precise information on the metal–proton distance can be derived as well. These essential parameters directly correlate with the efficacy of MRI contrast agents and should therefore aid the development of novel, highly efficient compounds targeted to various proteins.
Adsorptive Stripping Voltammetric Determination of Trace Uranium with a Bismuth‐Film Electrode Based on the U(VI)→U(V) Reduction Step of the Uranium‐Cupferron Complex
Authors: Kefala Georgia , Economou Anastasios , Voulgaropoulos Anastasios 
Published: November 2005

AbstractThis work reports the use of adsorptive stripping voltammetry (AdSV) for the determination of uranium on a preplated rotating‐disk bismuth‐film electrode (BiFE). The principle of the method relied on the complexation of U(VI) ions with cupferron and the subsequent adsorptive accumulation of the complex on the surface of the BiFE. The uranium in the accumulated complex was then reduced by means of a cathodic voltammetric scan while the analytically useful U(VI)→U(V) reduction signal was monitored. The experimental variables as well as potential interferences were investigated and the figures of merit of the method were established. Using the selected conditions, the 3σ limit of detection for uranium was 0.1 μg L−1 at a preconcentration time of 480 s and the relative standard deviation was 4.7% at the 5 μg L−1 level for a preconcentration time of 120 s (n=8). The accuracy of the method was established by analyzing a reference sea water sample.
Reduction of vividness and associated craving in personalized food imagery
Authors: McClelland Andrew , Kemps Eva , Tiggemann Marika 
Published: November 2005

AbstractThis study aimed to extend recent experimental work on the efficacy of visuo‐spatial working memory–based techniques for reducing food cravings by adopting a more naturalistic methodology. Fifty undergraduate women formed images of their favorite foods while performing a visuo‐spatial task across six successive trials. Vividness and craving intensity were rated for each food image. Concurrent visuo‐spatial processing reduced the vividness of, and craving reactivity to, personally relevant food images. Forehead tracking, a novel self‐administered task, proved to be as effective in reducing vividness and craving ratings as the established visuo‐spatial working memory laboratory tasks of eye movements, dynamic visual noise, and spatial tapping, and thus presents a simple, accessible technique potentially applicable in the home environment. All four tasks maintained their reducing effect over multiple trials. Individual differences in imaging ability and habitual food craving did not impact upon their effectiveness, indicating that visuo‐spatial tasks can be successfully used to reduce food cravings across a range of people. © 2005 Wiley Periodicals, Inc. J Clin Psychol 62: 355–365, 2006.
A two‐stage analysis of repeated measurements with dropouts and/or intermittent missing data
Authors: Overall John E. , Tonidandel Scott 
Published: November 2005

AbstractThis article is about a simple two‐stage analysis that utilizes slope coefficients as the dependent variable for testing the significance of difference in mean rates of change in repeated measurement designs with missing data. The ANCOVA test on the doubly weighted slope coefficients provides power comparable to that of more complex maximum likelihood procedures when data are missing completely at random, requires fewer assumptions and is more generally applicable under realistic nonrandom dropout conditions, and most importantly can be readily understood and explained by those who actually do most controlled clinical research. © 2005 Wiley Periodicals, Inc. J Clin Psychol 62: 285–291, 2006.
The innovation triad: an EvoDevo agenda
Authors: Müller Gerd B. , Newman Stuart A. 
Published: November 2005

AbstractThis article introduces a special issue on evolutionary innovation and morphological novelty, two interrelated themes that have received a remarkable increase of attention over the past few years. We begin with a discussion of the question of whether innovation and novelty represent distinct evolutionary problems that require a distinct conceptualization. We argue that the mechanisms of innovation and their phenotypic results—novelty—can only be properly addressed if they are distinguished from the standard evolutionary themes of variation and adaptation, and we present arguments for making such a distinction. We propose that origination, the first formation of biological structures, is another distinct problem of morphological evolution, and that together with innovation and novelty it constitutes a conceptual complex we call the innovation triad. We define a problem agenda of the triad, which separates the analysis of the initiating conditions from the mechanistic realization of innovation, and we discuss the theoretical problems that arise from treating innovation as distinct from variation. Further, we categorize the empirical approaches that address themes of the innovation triad in recognizing four major strands of research: the morphology and systematics program, the gene regulation program, the epigenetic program, and the theoretical biology program. We provide examples of each program, giving priority to contributions in the present issue. In conclusion, we observe that the innovation triad is one of the defining topics of EvoDevo research and may represent its most pertinent contribution to evolutionary theory. We point out that an inclusion of developmental systems properties into evolutionary theory represents a shift of explanatory emphasis from the external factors of natural selection to the internal dynamics of developmental systems, complementing adaptation with emergence, and contingency with inherency.J. Exp. Zool. (Mol. Dev. Evol.) 304B:487–503, 2005. © 2005 Wiley‐Liss, Inc.
Antibody responses against SARS coronavirus are correlated with disease outcome of infected individuals
Authors: Zhang Linqi , Zhang Fengwen , Yu Wenjie , He Tian , Yu Jian , Yi Christopher E. , Ba Lei , Li Wenhui , Farzan Michael , Chen Zhiwei , Yuen Kwok‐Yung , Ho David 
Published: November 2005

AbstractMost of the SARS‐CoV‐infected patients spontaneously recovered without clinical intervention while a small percentage succumbed to the disease. Here, we characterized temporal changes in N protein‐specific and S glycoprotein‐specific neutralizing antibody (Nab) responses in infected patients who have either recovered from or succumbed to SARS‐CoV infection. Recovered patients were found to have higher and sustainable levels of both N protein‐specific and S glycoprotein‐specific Nab responses, suggesting that antibody responses likely play an important role in determining the ultimate disease outcome of SARS‐CoV‐infected patients. J. Med. Virol. 78:1–8, 2006. © 2005 Wiley‐Liss, inc.
Impact of unreported HIV‐1 reverse transcriptase mutations on phenotypic resistance to nucleoside and non‐nucleoside inhibitors
Authors: Saracino A. , Monno L. , Scudeller L. , Cibelli D.C. , Tartaglia A. , Punzi G. , Torti C. , Lo Caputo S. , Mazzotta F. , Scotto G. , Carosi G. , Angarano G. 
Published: November 2005

AbstractAn extended spectrum of HIV‐1 reverse‐transcriptase (RT) mutations in HAART‐treated patients has been recently described. To verify the possible association of previously unreported RT mutations with a decrease of phenotypic susceptibility to nucleoside (NRTIs) and non‐nucleoside (NNRTIs) RT inhibitors, the RT sequence of 328 HIV‐1‐positive patients (102 naïve and 226 treated with HAART participating in either the PhenGen or Genpherex study) was analyzed. All treated patients were tested at the time of therapeutic failure with both phenotypic (Antivirogram®, Virco) and genotypic analyses (VircoGen™); the frequency of RT substitutions (positions 1–240) with respect to consensus B was compared to that of naïve patients using a Chi‐square test. Amino acid changes at 13 positions not included in the IAS list of resistance‐associated mutations were detected more frequently in treated than in naïve subjects. The mutations involving 10 of these positions were associated with a reduced susceptibility to antiretroviral drugs; K20R, T39A, K43EQN, E203KD, H208Y, and D218E were correlated with NRTI resistance while mutations K101EQP, H221Y, K223EQ, L228HR were associated to NNRTI resistance. A correlation was found between K20R and lamivudine resistance (P = 0.006) while T39A (P = 0.005), K43EQN (<0.001), E203KD (P = 0.010), and H208Y (P =  < 0.001) seemed to be associated with a previous use of zidovudine and stavudine and with the development of thymidine analog resistance. For H208Y, an association with use/resistance to abacavir (P = 0.004) was also noted. D218E showed a weak association to didanosine resistance (P = 0.013). The data confirm that previously unreported mutations are associated with antiretroviral drug experience and, more importantly, with a reduced susceptibility to NRTIs and NNRTIs. J. Med. Virol. 78:9–17, 2006. © 2005 Wiley‐Liss, inc.
Tracing the history of hepatitis B virus genotype D in western Japan
Authors: Michitaka Kojiro , Tanaka Yasuhito , Horiike Norio , Duong Tran Nhu , Chen Yan , Matsuura Kana , Hiasa Yoichi , Mizokami Masashi , Onji Morikazu 
Published: November 2005

AbstractThe major hepatitis B virus (HBV) genotypes in Japan are B and C. HBV genotype D (HBV/D), however, is widespread in a small area of Western Japan, where the Gianotti–Crosti syndrome caused by HBV subtype ayw, which is suspected to be HBV/D, was endemic in the 1970s. The aim of the study was to elucidate its origin, time of transmission, and spread in this area. Genotyping of HBV‐DNA was done in 363 patients with HBV infection. The year of birth was checked in patients with HBV/D. The full genome sequences of 20 HBV/D strains, 2 of which were obtained from a single carrier with a 19‐year‐interval, were analyzed. An evolutionary rate, the date of the most recent common ancestor, and the effective number of HBV/D infections were calculated. Fifty‐two of 363 patients were infected with HBV/D, and 39 were born in 1970s. In a phylogenetic tree, the 20 HBV/D strains produced a definite cluster, and the evolutionary rate was calculated to be 5.4 × 10−5 nucleotide substitutions/site/year. The root of the tree was estimated to be in approximately 1,900 and began to spread from the 1940s, leading to a rapid increase of infected patients in the 1970s. From these results, it is suspected that HBV/D was likely transmitted to the area investigated approximately 100 years ago and then spread widely in the 1970s. From the history of the area and the genetic analysis, HBV/D in this area was speculated to be of Russian origin. J. Med. Virol. 78:44–52, 2006. © 2005 Wiley‐Liss, inc.
Patients with and without loss of hepatitis B virus DNA after hepatitis B e antigen seroconversion have different virological characteristics
Authors: Misawa Noriko , Matsumoto Akihiro , Tanaka Eiji , Rokuhara Akinori , Yoshizawa Kaname , Umemura Takeji , Maki Noboru , Kimura Tatsuji , Kiyosawa Kendo 
Published: November 2005

AbstractThe characteristic differences between patients with and without loss of hepatitis B virus (HBV) DNA after achieving hepatitis B e antigen seroconversion were analyzed by comparing changes in HBV DNA and HBV core‐related antigen levels during a period from 3 years before to 3 years after the seroconversion. Of the 24 seroconverters, 6 (inactive replication group) showed continuous loss of HBV DNA in serum after the seroconversion and the remaining 18 did not lose HBV DNA (active replication group). The HBV DNA level was similar between the two groups, while the HBV core‐related antigen level was significantly lower in the active replication group than in the inactive replication group before the seroconversion. The levels of both HBV DNA and HBV core‐related antigen decreased remarkably around the time of seroconversion in the inactive replication group, while these levels did not change or decreased slightly in the active replication group. After the seroconversion, the HBV DNA level was significantly higher in the active replication group than in the inactive replication group, while the HBV core‐related antigen level was similarly low between the two groups. Because the serum level of HBV core‐related antigen mainly reflects that of HBe antigen, the low level of HBV core‐related antigen seen after seroconversion in both groups might have contributed to the occurrence of seroconversion. The precore and core promoter mutations which cause diminished excretion of hepatitis B e antigen were significantly more frequent in the active replication group than in the inactive replication group. It was therefore considered that the seroconversion was caused mainly by a decrease in viral replication in the inactive replication group, and mainly by a decrease in HBe antigen production in the active replication group. J. Med. Virol. 78:68–73, 2006. © 2005 Wiley‐Liss, inc.
Rapid and simple detection of IFN‐neutralizing antibodies in chronic hepatitis C non‐responsive to IFN‐α
Authors: Jorns Carl , Holzinger Dirk , Thimme Robert , Spangenberg Hans Christian , Weidmann Manfred , Rasenack Jens , Blum Hubert Erich , Haller Otto , Kochs Georg 
Published: November 2005

AbstractDifferent mechanisms have been proposed for the failure of interferon (IFN) therapy in patients with chronic hepatitis C and multiple sclerosis, for example, the presence of IFN‐neutralizing antibodies. In this study, a novel assay system based on the IFN‐inducible Mx‐promoter was used to detect IFN‐neutralizing antibodies in sera of patients with chronic hepatitis C. To monitor IFN bioactivity in IFN‐treated patients, a real‐time RT‐PCR for MxA gene expression in PBMCs was established. Using these two methods, patients with chronic hepatitis C virus (HCV) infection receiving IFN therapy and patients with treatment induced HCV clearance were monitored. Importantly, neutralizing anti‐IFN antibodies were detected in the sera of 3 of 38 chronically HCV‐infected patients who failed to respond to therapy but none in sera of patients who cleared HCV after IFN therapy. Interestingly, the presence of these antibodies correlated with the lack of MxA induction in PBMCs after initiation of IFN‐α therapy. Retrospective analysis of one patient's sera revealed that the anti‐IFN‐α antibodies had already developed after the first of four unsuccessful IFN therapies, suggesting that neutralizing antibodies may have contributed to the failure of previous IFN treatments. In summary, a novel screening assay was established that may be helpful for testing IFN non‐responders for the presence of clinically relevant anti‐IFN‐α antibodies and for selecting alternative IFN preparations not neutralized by these antibodies. J. Med. Virol. 78:74–82, 2006. © 2005 Wiley‐Liss, inc.