bluuebunny/crossref_metadata_2025_split huggingface dataset:
DNA Flow Cytometry Analysis in Body Cavity Fluids Using Liquid‐Based Cytology
Authors: Ryu Ayumi , Honma Keiichiro , Tsuzaki Sayoko , Yoshioka Risa , Tanada Satoshi , Yamamoto Takashi , Satomi Hidetoshi , Nagata Shigenori , Yamasaki Tomoyuki , Ohue Masayuki 
Published: February 2025

ABSTRACTBackgroundDNA flow cytometry using LC‐1000 is utilized for analyzing cellular and nuclear lengths and DNA content in individual cells with samples in cell preservation solutions to acquire the cell proliferation index (CPIx). This study aimed to evaluate the performance of body cavity fluid cytology using LC‐1000. As cell preservation solutions, liquid‐based cytology (LBC) by Cellprep (CP) was compared with CelVerse (CeV), specifically designed for the LC‐1000.MethodsOverall, we evaluated 43 samples (20 malignant and 23 benign) of body cavity fluids suspended in CeV and 59 samples (28 malignant and 31 benign) suspended in CP. The CPIx was compared between malignant and benign cases of CeV and CP. Further, the subject groups were divided according to the SubG1% in a sample.ResultsThe CPIx in CeV was 0.14–10.22 (median 0.62) in malignant cases and 0.09–4.64 (median 0.19) in benign cases (p < 0.001); additionally, CP displayed 0.14–9.87 (median 0.84) in malignant cases and 0.11–3.02 (median 0.22) in benign cases (p < 0.001). The area under the curve (AUC) was 0.811 for CeV and 0.776 for CP. The difference in SubG1% between the CeV and CP groups was statistically significant (p < 0.0001) and influenced the AUC in CP.ConclusionThe CPIx in CeV or CP samples using the LC‐1000 may be applicable as an objective indicator in body cavity fluid cytology. Samples preserved in CeV provided more consistent results, unaffected by SubG1%, when compared to those stored in CP.
Towards a Consensus for Dyslexia Practice: Findings of a Delphi Study on Assessment and Identification
Authors: Holden Caroline , Kirby Philip , Snowling Margaret J. , Thompson Paul A. , Carroll Julia M. 
Published: February 2025

ABSTRACTThis paper discusses the findings of a Delphi study in which dyslexia experts, including academics, specialist teachers, educational psychologists, and individuals with dyslexia, were asked for their agreement with a set of key statements about defining and identifying dyslexia: why it should be assessed and how and when this assessment should be conducted. Two rounds of survey responses provided a vehicle for moving towards consensus on how to assess for dyslexia. Forty‐two consensus statements were ultimately accepted. Findings suggested that assessment practice should take account of risks to the accurate identification of dyslexia. An assessment model, with guidelines for assessors, is presented, based on the Delphi's findings. This hypothesis‐testing model requires assessors to investigate and weigh up the factors most likely to result in an accurate assessment before reaching conclusions, assigning terminology, and making recommendations for intervention and management.
Historical Demography and Species Distribution Models Shed Light on Speciation in Primates of Northeast India
Authors: Trivedi Mihir , Arekar Kunal , Manu Shivakumara , Kuderna Lukas F. K. , Rogers Jeffrey , Farh Kyle Kai‐How , Bonet Tomas Marques , Umapathy Govindhaswamy 
Published: February 2025

ABSTRACTPast climate change is one of the important factors influencing primate speciation. Populations of various species could have risen or declined in response to these climatic fluctuations. Northeast India harbors a rich diversity of primates, where such fluctuations can be implicated. Recent advances in climate modeling as well as genomic data analysis has paved the way for understanding how species accumulate at a particular geographic region. We utilized these methods to explore the primate diversity in this unique region in relation to past climate change. To ascertain the population level changes, we inferred the demographic history of nine species of primates found in Northeast India and compared it with species distribution models of Pliocene and Pleistocene period. Through this study, we are able to provide a detailed picture of how past climatic changes have resulted in the present species diversity and this mixture of species have either originated in the region or have dispersed from mainland Southeast Asia. We observe that effective population size has decreased for all the species, but distributions are different for all the four genera: Macaca, Trachypithecus, Hoolock and Nycticebus. It also gives an idea about how each species is affected differently by climate change, and why it should be given emphasis in framing species‐wise conservation models for future climate change.
Grassland bud and shoot demographic responses to single and recurrent droughts vary across an aridity gradient
Authors: Te Niwu , Griffin‐Nolan Robert J. , Slette Ingrid J. , Shi Yuan , Song Lin , Chen Jiaqi , Wang Hongqiang , Qian Jianqiang , Yu Qiang , Wang Zhengwen , Lü Xiaotao , Han Xingguo , Klimešová Jitka , Collins Scott L. , Luo Wentao 
Published: February 2025

AbstractClonal demographic traits play important roles in regulating community dynamics. Yet, it remains unclear how the responses of these clonal traits to drought might depend on previous drought exposure, and how drought responses vary among grasslands. We conducted a repeated drought experiment across four grasslands along an aridity gradient in northern China. We assessed the effects of single (precipitation reduction in 2021–2022) versus recurrent (precipitation reduction in 2015–2018 and 2021–2022) drought on bud density, shoot density, and the ratio of bud to shoot density. Drought reduced bud density at all grasslands and shoot density at most grasslands. Drought reduced the ratio of bud‐to‐shoot density only in the most arid grasslands. Recurrent drought had larger negative effects than a single drought on bud density and composition of bud and shoot at only one of four grasslands, and on shoot density at two of four grasslands. Our results suggest that previous drought exposure can alter the response of plant clonal demographic traits to subsequent drought in some but not all grasslands, and that responses can vary with mean climate.
Serum Vitamin D Levels and Risk of Iron Deficiency Anemia in Adults: A Cross‐Sectional Study and Mendelian Randomization Analysis
Authors: Chen Leifei , Gu Nanyuan , Qiu Kai , Chen Hui , Tian Fu , Chen Yang , Zeng Longhuan 
Published: February 2025

ABSTRACTThis study focuses on the potential role of vitamin D in the diagnosis and treatment of iron deficiency anemia (IDA), and evaluates causation using Mendelian randomization (MR). This cross‐sectional study collected clinical information from 3728 respondents in the National Health and Nutrition Examination Survey (NHANES) cycles of 2005–2010 and 2015–2018. To determine the link between serum vitamin D levels and the risk of IDA, we established a robust nomogram. Calibration and net clinical benefits were examined through calibration curves and decision curve analysis (DCA). Restricted cubic spline (RCS) models were utilized to explore the relationship between the two. In addition, the data of single nucleotide polymorphism (SNP) related to vitamin D and IDA were obtained from open biological databases. The main analytical method for the MR analysis was the inverse variance weighted (IVW) method. A series of sensitivity analyses were conducted to evaluate pleiotropy. Our cross‐sectional study showed that, after extensive adjustments, serum vitamin D levels remained an independent risk factor for predicting the development of IDA. The risk of developing IDA was significantly lower for participants in the highest quartile subgroup with vitamin D levels ≥ 78.1 nmol/L compared to those in the lowest quartile with vitamin D levels ≤ 42.8 nmol/L (p < 0.001). In terms of gender, serum vitamin D primarily exhibited a protective effect against IDA in females (OR:0.98, 95% CI: 0.98–0.99, p < 0.001). A non‐linear relationship between the two was found (p < 0.001 for non‐linear relationship). Meanwhile, using the IVW method in MR analysis, we identified a bidirectional causal relationship. The results of our cross‐sectional analysis demonstrated a negative correlation between serum vitamin D levels and IDA. Additionally, genetic evidence from the MR analysis supported an association between serum vitamin D levels and IDA.
Comprehensive Assessment of Technological Challenges In Photovoltaic Waste Recovery In India Using Principal Component Analysis and Analytic Hierarchy Process Models
Authors: Yadav Dinesh , Kumar Sanjeev , Paramasivam Prabhu , Kanti Praveen Kumar , Gupta Rupesh , Yusuf Mohamed 
Published: February 2025

AbstractThe rapid expansion of photovoltaic (PV) technology has raised concerns about sustainable PV waste management, particularly in India, where inadequate infrastructure and technical limitations hinder effective recycling. Addressing these challenges is crucial for minimizing environmental risks and promoting a circular economy in the renewable energy sector. This study presents a smart multi‐criteria decision‐making (MCDM) approach that integrates Principal Component Analysis (PCA) and the Analytic Hierarchy Process (AHP) to assess technological challenges in PV waste management. PCA is applied to prioritize key challenges, while AHP evaluated their interrelationships through criteria weights. Despite the effectiveness of PCA and AHP, their combined application in PV waste recovery remains underexplored, particularly in the Indian context. Eight key challenges are identified, with hazardous recycling methods (83.2%) and low recycling potential (83.4%) ranking highest in PCA. AHP results highlighted the lack of advanced recycling technology (0.2298) and hazardous recycling methods (0.2084) as the most critical barriers. A multi‐criteria utility function is developed to illustrate these interdependencies. This research bridges critical knowledge gaps by offering data‐driven insights into PV waste recovery in India, contributing to sustainable waste management strategies and the development of an efficient recycling framework.
Alternative MYB Promoter Activity Is a Potential Prognostic Biomarker in Head and Neck Adenoid Cystic Carcinoma
Authors: Jäwert Fredrik , Fehr André , Persson Marta , El‐Naggar Adel K. , Stenman Göran , Andersson Mattias K. 
Published: February 2025

ABSTRACTBackgroundAdenoid cystic carcinoma (ACC) is an aggressive therapy‐resistant head and neck cancer with a high frequency of local recurrences and distant metastases. Activation of the MYB gene, encoding an oncogenic master transcription factor, is the key genomic hallmark of ACC and a potential therapeutic target. Here, we have investigated the clinical significance of alternative MYB promoter (MYB TSS2) activation in primary ACCs.MethodsMYB TSS2 activity was studied in 28 ACCs using MYB exon‐specific qPCR assays. Overall survival (OS) and risk analyses were done to estimate the outcomes of MYB TSS2 high and low cases, respectively. The genomic and transcriptomic profiles of the ACCs were also analyzed in relation to MYB TSS2 activity.ResultsOS was significantly shorter among patients in the MYB TSS2 high group compared to the MYB TSS2 low group (p = 0.02). Multivariate analysis indicated that MYB TSS2 activity was a significant prognostic biomarker for OS (p = 0.03) that was independent of tumor grade and NOTCH1 mutation status. There was markedly higher MYB TSS2 activity in ACCs with 6q deletions compared to those without deletions (p = 0.04). Moreover, MYB TSS2 high tumors had an increased expression of cell cycle genes and known MYB target genes.ConclusionThe study demonstrates that alternative MYB promoter activity is a new potential prognostic biomarker for ACC and further strengthens the relevance of MYB‐targeted therapy. Our findings indicate that MYB TSS2 high primary tumors are more aggressive and have a worse prognosis than MYB TSS2 low tumors.
Targeting the crosstalk between glutamine metabolism and tumor immune microenvironment for lung cancer immunotherapy
Authors: Li Jingyang , Xiang Li , Wang Shaohui , Zhang Yingrui , Zhao Shiyi , Zhang Di , Meng Xianli , Zhang Yi , Lin Jin‐Ming 
Published: February 2025

AbstractLung cancer remains one of the deadliest cancers globally, which may be attributed in part to a limited understanding of its development. Therefore, exploration of the fundamental processes of lung cancer and innovative therapies is imminent. Recently, lung cancer immunotherapy has become a crucial topic of interest, and it pertains to the metabolism in lung tumor microenvironment. In particular, glutamine metabolism plays a pivotal role in lung cancer immunotherapy. This review summarizes the crosstalk between glutamine metabolism and the lung tumor immune microenvironment, and explores potential strategies of glutamine metabolism as an enhancement of lung cancer immunotherapy. It also discusses the potential of traditional Chinese medicine in regulating glutamine metabolism in lung cancer immunotherapy. This review may offer valuable insights and promising directions for future studies on this subject.
Experimental Kinetic Study of the Reactions Between NO3 Radicals and α‐ and β‐Phellandrenes
Authors: Harb Sergio , Cirtog Manuela , Cazaunau Mathieu , Pangui Edouard , Bergé Antonin , Picquet‐Varrault Bénédicte 
Published: February 2025

ABSTRACTNighttime NO3‐initiated oxidation of biogenic volatile organic compounds (BVOCs), such as monoterpenes, plays a crucial role as sources of organic nitrates (ONs) and secondary organic aerosols (SOA), which are known to have significant impacts on climate, air quality, and human health. Nevertheless, these reactions are still poorly understood. Therefore, the primary objective of this study is to provide new kinetic data for two monoterpenes, α‐phellandrene and β‐phellandrene through experiments in simulation chambers. The rate constants have been determined using an absolute kinetic method and found to be (3.9 ± 0.6) × 10−11 cm3 molecule−1 s−1 for α‐phellandrene and (6.6 ± 1.0) × 10−12 cm3 molecule−1 s−1 for β‐phellandrene. These rate constants have been compared to data from the literature. For α‐phellandrene, previous studies were scattered, and this new determination allows us to confirm the lowest value provided by the other absolute rate determination. For β‐phellandrene, our study provides the first absolute rate determination which is in good agreement with the unique value from the literature obtained by the relative rate technique. Rate constants of α‐ and β‐phellandrene are also compared to those of other monoterpenes having similar chemical structures. These kinetic results show that the oxidation by NO3 is a significant sink of α‐ and β‐phellandrene during nighttime.
Impacts of Land Tenure Security on the Conversion of Agricultural Land to Urban Use
Authors: Movahhed Moghaddam Saghi , Azadi Hossein , Sklenička Petr , Janečková Kristina 
Published: February 2025

ABSTRACTLand tenure security influences several processes relevant to the long‐term sustainability of farmland management, including agricultural land conversion to urban use (ALCU). This phenomenon has been illustrated by several studies, mainly on the scale of individual countries. However, there is a noticeable lack of global‐scale analyses examining how different aspects of land tenure affect ALCU. To address this gap, we have conducted a meta‐analysis of 62 studies that provide quantitative insights into the effects of land tenure variables on ALCU. We used Comprehensive Meta‐Analysis (CMA) software to extract data from original articles, including land tenure type (expressed as land farmed by its owner and land farmed by a tenant), legal status (titled land and untitled land), cadastral assignment (designated and not‐designated agricultural land), and land ownership (private land and communal land). We have found significant effects of all these aspects of land tenure on ALCU. Land farmed by its owner was the most substantial factor protecting agricultural land from urbanization, with a 3.42% decrease in ALCU. We also observed a 2.97% decrease in ALCU on titled land, a 2.62% decrease on designated agricultural land, and a 1.85% decrease on land that was privately owned. The application of findings and implications for policymakers are substantial: (i) efforts to secure land tenure, especially in underinvested areas, can minimize the degradation of land resources and facilitate better farmland preservation, (ii) the support of owner‐operated farming, land titles, private ownership of land, and designated agricultural land is conducive to sustainable farmland management, and (iii) the synergic effect of promoting land tenure security should be utilized to protect agricultural land from conversion to urban uses.
Endoscopic Ultrasound‐Guided Brachytherapy of Yttrium‐90 Implantation Into Pancreas: A Dose‐Escalation Pilot Study
Authors: Zhao Yuchong , Yang Yilei , Zhang Buchuan , Cui Haochen , Liu Luyao , Wang Ronghua , Han Yunfeng , Zhu Dongling , Ma Wenliang , Zhang Xinxing , Wang Jinlin , Xiong Si , Bai Shuya , Zhu Xiaohua , Cheng Bin 
Published: February 2025

ABSTRACTIntratumoral brachytherapy enables higher dose treatment and reduces damage to adjacent tissues. We first validated the feasibility and safety of endoscopic ultrasound (EUS)‐guided Yttrium‐90 (90Y) microspheres implantation in a porcine model. Under EUS guidance, 90Y‐loaded microspheres were implanted into the pancreas of 10 miniature pigs. The first pig was implanted with 10 MBq particles. Subsequently, nine pigs were sequentially included in the low‐ (20 MBq), medium‐ (40 MBq), and high‐dose (60 MBq) groups. Positron emission tomography (PET)/CT imaging was used to check the occurrence of particle displacement postoperatively. After euthanasia, the pancreas and adjacent organs were excised for histological examination and residue radiation detection. The absorbed doses demonstrated safe in the porcine model were further in the xenograft model and KRASLSL/+Trp53FL/FLPtfqaCre/+ mouse model. EUS‐guided implantations of 90Y‐loaded microspheres were successful in all animals. Two pigs had mild serum amylase elevation in the high‐dose group and the abnormal index returned to baseline without interventions. The volume of necrotic lesions ranged from 255.76 to 745.57 mm3. In KPC mouse model, PET/CT imaging demonstrated a significant decrease in maximum standardized uptake value (SUVmax) after 90Y implantation. EUS‐guided 90Y‐loaded carbon microsphere implantation could serve as a safe and feasible technique at ultrahigh dose for pancreatic cancer brachytherapy.
Modeling Immobilized Enzyme Reactions: Nonlinear Kinetics With Fractional‐ and Integer‐Order Analysis
Authors: Rajaraman R. 
Published: February 2025

ABSTRACTThis research investigates the immobilization of enzymes within porous materials of varying geometries, such as spherical and cylindrical pellet‐shaped catalysts. The study focuses on modeling enzyme reactions using reaction–diffusion equations that capture the irreversible Michaelis–Menten kinetics, emphasizing the nonlinear nature of the process. A distinctive feature of this work is the incorporation of fractional derivatives to enhance the understanding of enzymatic reaction kinetics. To achieve this, a novel computational framework utilizing Laguerre wavelets is developed to compute substrate concentrations and effectiveness factors over a broad range of parameter values. The proposed Laguerre wavelet method (LAWM) is rigorously compared against established analytical and numerical approaches, including the Hermite wavelet method (HWM), Taylor series method (TSM), Adomian decomposition method (ADM), and the fourth‐order Runge–Kutta method (RKM). The findings reveal a high degree of accuracy and consistency across all methods, underscoring the reliability and efficiency of the LAWM. This study offers new insights into enzyme kinetics within porous catalysts and highlights the potential of fractional‐order models for advancing biocatalytic applications. The outcomes provide a robust theoretical foundation for optimizing the design and performance of immobilized enzyme reactors in industrial and biotechnological settings.
Efficient and Rapid Histomorphometry of Regenerating Peripheral Nerve
Authors: Yau Jenny , Hernández Iván Coto , Minderler Steven , Barrera Keisha , Jowett Nate 
Published: February 2025

ABSTRACTIntroduction/AimsPeripheral nerve surgery entails repairing or transferring nerves to restore sensory or motor functions. Functional outcomes correlate with axon counts within operated nerves, but there exists no reliable means for intraoperative assessment of injured nerves. Conventional histologic assessment of regenerating peripheral nerve is labor‐intensive, requiring multiday processing for tissue immunohistochemistry. Here, we describe a high‐throughput protocol for histomorphometry of regenerating peripheral nerves.MethodsHealthy and regenerating murine and human peripheral nerve sections were stained with commercial fluorescent membrane‐specific dyes, one of which (MemBrite) was discovered to internalize within axons. Samples were imaged using confocal microscopy, and regenerating axons were quantified using commercial segmentation software. Quantification results using the novel staining protocol were compared against conventional neurofilament immunohistochemistry. The protocol was employed to guide intraoperative decision‐making in a case of free functional muscle transfer for treating a patient with facial paralysis.ResultsAxon quantification within healthy and regenerating nerve sections in human and murine samples via rapid staining yielded similar results when compared to antineurofilament labeling. The relative difference in axon counts for the healthy murine facial nerve for antineurofilament and MemBrite 594 staining was < 1%. For the regenerating human nerve, the relative difference was 8.7%.DiscussionA rapid quantification protocol for regenerating axons has been described, which may inform intraoperative decision‐making and advance knowledge in peripheral nerve surgery. Minimizing tissue processing time during surgery would allow surgeons to receive immediate feedback regarding axon counts in the donor nerve for cross‐facial nerve surgeries, ultimately leading to improved clinical outcomes for their patients.