bluuebunny/crossref_metadata_2025_split huggingface dataset:
Connective tissue activation. xxxi. identification of two molecular forms of a human mesenchymal cell—derived growth factor, connective tissue activating peptide—V
Authors: Cabral A. R. , Castor C. W. 
Published: February 2007

AbstractWe previously identified, in normal urine, a growth factor that stimulated monolayer cultures of human synovial, cartilage, and dermal fibroblasts to synthesize incremental amounts of hyaluronic acid, proteoglycans, and DNA. An isolation procedure guided by bioassays and immunologic methods disclosed 2 anionic bioactive polypeptides with Mr of 28,000 and 16,000, respectively, as judged by single bands with sodium dodecyl sulfate—polyacrylamide gel electrophoresis in reduced and nonreduced samples. Rabbit antibodies raised against each purified protein were shown to react, on immunodiffusion and Western blot, with both antigens. Immunohistochemical and immunobinding studies detected the protein in normal human synovial, dermal, and cartilage fibroblasts and in human saphenous vein endothelial cells. The mesenchymal cell—derived growth factor is now designated connective tissue activating peptide—V (CTAP‐V). Monospecific polyclonal anti—CTAP‐V antibodies were used in a radial immunodiffusion assay for quantitative determination of the antigen in biologic fluids. In normal human plasma the concentration of CTAP‐V was below the limit of detection. The CTAP‐V concentration in normal urine was 4.5 ± 2.0 m̈g/ml, calculated from measurements of 5—18‐fold concentrated samples. Joint fluid from patients with rheumatic diseases and normal renal function had CTAP‐V levels similar to those found in plasma; 2—15‐fold increases were detected in plasma and joint fluid of patients with chronic renal failure. Immunodiffusion or dot‐blot analysis revealed a CTAP‐V—like material in the plasma or serum of 10 mammalian species. It was not detectable in 2 avian species.
Correlates of community resource information needs of arthritis patients
Authors: Hull Alan L. , Liebman Judith J. , Moskowitz Roland W. 
Published: February 2007

AbstractOne hundred eighty‐nine ambulatory arthritis patients were interviewed to assess needs for information about community resource services. Participants were asked how frequently they needed 66 specific information items. Three categories of resource needs in arthritis care were identified: general information, personal care and medical services, and assistive devices and equipment. In the first category, results indicated that the greater the impairment in functional ability, the greater the need for general information about arthritis. When functional ability was held constant, younger patients with lower family income displayed a greater need for arthritis resource information. Lower functional ability and family income were associated with a greater need for personal care and medical service information. Lower functional ability contributed the most to the need for information about assistive devices and equipment. We suggest that, as an essential component of the long‐term care of patients with rheumatic diseases, individuals who provide educational counseling should clearly understand the community resource needs of patients, and should be able to match articulated needs with the existing services. Health professionals should be aware that certain patient characteristics (specifically, family income, functional ability, and age) are related to a need for this type of information. The large percentage of patients who could not name their rheumatic disease diagnosis (65%) presents a major concern and challenge to patient educators.
Detection of anticentromere antibodies using cloned autoantigen cenp‐Bl
Authors: Rothfield N. , Whitaker D. , Bordwell B. , Weiner E. , Senecal J.‐L. , Earnshaw W. 
Published: February 2007

AbstractA solid‐phase enzyme‐linked immunosorbent assay has been established using a cloned fusion protein, Cterm CENP‐B [β‐gal], as antigen. The fusion protein carries the major epitope of CENP‐B, the major centromeric autoantigen. The enzyme‐linked immunosorbent assay was more sensitive than immunofluorescence techniques in detecting anticentromere antibodies in patients with scleroderma or Raynaud'S disease, and was weakly positive in 3% of normal controls and in 3% of 70 patients with other connective tissue diseases.
A dr4‐associated dr‐dq haplotype is significantly associated with rheumatoid arthritis
Authors: Wallin Johan , Carlsson BjöUrn , Ströum HÅRkan , Möuller Erna 
Published: February 2007

AbstractForty‐three patients with seropositive, erosive rheumatoid arthritis (RA) and 24 members of 5 RA multicase families were studied for HLA class II gene polymorphism, using restriction fragment analysis with complementary DNA probes for DRβ and DQβ chains. This method generates HLA‐DR‐DQ haplotypes that are highly correlated with HLA‐DR serology. Thirty‐five of the 43 RA patients (81%) were positive for one or for both of the DR4‐associated DR‐DQ haplotypes, 4.1 and 4.2. Among these patients, the 4.1 haplotype was found significantly more often than in DR4 + controls (P < 0.01). The haplotype segment C3;B15;DR4 was present in all RA patients in 4 of the 5 families, and included the DR‐DQ4.1 haplotype.
Inhibition of in vitro proliferative response of cultured t lymphocytes to interleukin‐2 by gold sodium thiomalate
Authors: Wolf Robert E. , Hall Vicky C. 
Published: February 2007

AbstractGold sodium thiomalate (GST), in concentrations attainable during chrysotherapy for rheumatoid arthritis, significantly inhibited the proliferative responses of cultured T cells stimulated by interleukin‐2 (IL‐2). The observed suppression was not related to altered kinetics, cell death, or interference with the binding of IL‐2 to cell surface receptors. It appeared that GST affected an early step in the proliferative process, since maximum inhibition was obtained by the addition of GST within 4 hours of stimulation; progressive reduction of suppression was observed when GST was added later. Significant inhibition occurred when cultured T cells were preincubated for 24 hours with GST and washed prior to IL‐2 stimulation, although the degree of suppression was decreased. Thus, inhibitory activity was not dependent on the continued presence of GST throughout culture. These findings suggest that there is a direct inhibition of T lymphocytes by GST, which may be important in immunomodulation by gold compounds.
An enzymatically induced structural transformation in articular cartilage. its significance with respect to matrix breakdown
Authors: Broom Neil D. 
Published: February 2007

AbstractIt was demonstrated in this study that the 3‐dimensional, “pseudo‐random” architecture of collagen in the general matrix of normal cartilage can be transformed enzymatically into a radial array of fibril aggregates or fibers. By first degrading the proteoglycans and then attacking the collagen, it is possible to produce a collagenous structure almost identical to that observed in matrices exhibiting both nonprogressive softening and osteoarthritic changes, and in matrices subjected to dynamic overloading. This structural transformation is explained as a breakdown in the fibril interlinking system.
Anti–ro (ss‐a) antibodies from ro (ss‐a)–immunized mice
Authors: Rosario Myra O. , Fox Owen F. , Koren Eugen , Harley John B. 
Published: February 2007

AbstractImmunization of BALB/c mice with immuno‐affinity‐purified bovine or human Ro (SS‐A) induces the production of antibodies reactive with Ro (SS‐A). Fusion of spleen cells from the hyperimmunized mice to SP2/0 cells resulted in hybridoma cell lines that produced anti–Ro (SS‐A) antibodies. Anti–Ro (SS‐A) binding was established by solid‐phase immunosorbent assay, immunoblotting, or RNA immunoprecipitation. Most of the anti–Ro (SS‐A) antibodies bound to both human and bovine Ro (SS‐A) in the solid phase, but only one of the monoclonal antibodies selectively bound to human Ro (SS‐A); this suggests that there are species differences between the bovine and human Ro (SS‐A) antigens. Indirect immunofluorescence studies demonstrated that most anti–Ro (SS‐A) antibodies bound to cytoplasmic or nuclear HEp‐2 cellular antigens, whereas others did not bind to fixed HEp‐2 tissue culture cells. Nuclear staining of mouse substrates by one of the sera containing anti–Ro (SS‐A) demonstrated that autoantibodies were induced by immunization with human Ro (SS‐A).
Antibodies to types i, ii, ix, and xi collagen in the serum of patients with rheumatic diseases
Authors: Charríre Guyĺne , Hartmann Daniel J. , Vignon Eric , Ronzíre Marie‐Claire , Herbage Daniel , Ville G´rard 
Published: February 2007

AbstractAntibodies to native types I, II, IX, and XI collagen were measured, using a 125I‐solid‐phase radio‐immunoassay, in serum from 104 patients with rheumatic diseases (rheumatoid arthritis, osteoporosis, Paget's disease, or osteoarthritis). In all disease groups, antibodies to type II collagen occurred with greater frequency than antibodies to type I collagen (11–35% versus 5–23%). Antibodies to type XI collagen were the most frequent: They were present in ˜50% of the patients in the rheumatoid arthritis, Paget's disease, and osteoporosis groups. Antibodies to type IX collagen were found at a high frequency in the rheumatoid arthritis group only (44%). Analysis of the clinical data suggested that the presence of antibodies to collagen was associated with disease that was less severe or of shorter duration.
Interstitial pneumonitis in antinuclear antibody–negative systemic lupus erythematosus: a new clinical manifestation and possible association with anti–ro (ss‐a) antibodies
Authors: Hedgpeth Madelaine T. , Boulware Dennis W. 
Published: February 2007

AbstractThe clinical manifestations of patients with systemic lupus erythematosus (SLE) and negative fluorescent antinuclear antibody have been well documented. This report describes 2 cases of antinuclear antibody–negative SLE with antibodies only to Ro (SS‐A) and the previously unreported clinical manifestation of lupus interstitial pneumonitis. A review of all SLE patients from 1984–1986 with interstitial pneumonitis revealed that 10 of the 12 patients had antibodies to Ro (SS‐A). The frequency of anti–Ro (SS‐A) with interstitial pneumonitis in SLE patients suggests a possible association between the two.
Increase in levels of serum keratan sulfate following cartilage proteoglycan degradation in the rabbit knee joint
Authors: Williams James M. , Downey Colleen , Thonar EUGENE J.‐M. A. 
Published: February 2007

AbstractFollowing injection of chymopapain into a single knee joint in rabbits, serum keratan sulfate levels rose sharply and remained elevated for at least 48 hours before returning to preinjection levels. These changes were accompanied by depletion of proteoglycans from articular cartilage in the injected joint. We conclude that serum keratan sulfate levels rise predictably following acute loss of proteoglycan from a single joint.
Frequency of infection among patients with rheumatoid arthritis versus patients with osteoarthritis or soft tissue rheumatism
Authors: van Albada‐Kuipers G. A. , Linthorst J. , Peeters E. A. J. , Breedveld F. C. , Dijkmans B. A. C. , Hermans J. , Vandenbroucke J. P. , Cats A. 
Published: February 2007

AbstractWe compared the frequencies and types of infections that occurred in 448 patients with rheumatoid arthritis (RA) versus those occurring in 185 control patients who had osteoarthritis or soft tissue rheumatism. At least 1 infection developed in 23% of the RA patients and in 27% of the control patients. Using a series of risk factors, we found no substantial difference in the risk of infection in RA patients compared with that in control patients. Some of these factors, such as being female, having poor functional capacity, and receiving glucocorticoid treatment, were associated with different levels of risk for infection, but the levels were similar in RA patients and control patients.
A model of health status for rheumatoid arthritis. a factor analysis of the arthritis impact measurement scales
Authors: Mason John H. , Anderson Jennifer J. , Meenan Robert F. 
Published: February 2007

AbstractHealth status is a key aspect of quality of life that may be addressed and affected by health care. The measurement of this construct requires the identification of unique and relevant components of health status that can be operationally defined by specific scales or scale groupings. We explored the components of health status that are operationalized by the Arthritis Impact Measurement Scales (AIMS). Prior testing has shown that the AIMS measures delineate at least 3 components of health status: Physical Function, Psychological Status, and Pain. This 3‐component model, however, can be theoretically and empirically restrictive. Using AIMS responses from a rheumatoid arthritis (RA) patient population, our investigation, through factor analyses, identifies 5 components of health status in this chronic disease: Lower Extremity Function, Upper Extremity Function, Affect, Symptom, and Social Interaction. It is proposed that these intuitively reasonable and measurable components constitute the core of the health status construct for individuals with RA. These 5 components could also prove central to the assessment of health status in other rheumatic diseases. Future studies of health status in RA should include tested measures to assess these 5 components.
What are we measuring? an examination of self‐reported functional status measures
Authors: Spiegel Jane Sinden , Leake Barbara , Spiegel Timothy M. , Paulus Harold E. , Kane Robert L. , Ward Nancy B. , Ware John E. 
Published: February 2007

AbstractFunctional status questionnaires are being used in various types of studies. To determine factors related to self‐reported functional ability for rheumatoid arthritis patients, we examined the relationship between a functional and mental health questionnaire and objective disease‐specific measures. Using 3–5 predictor variables, we explained 43–57% of the variance in patients' self‐reported functioning. Mental and physical health perceptions were significant predictors for each self‐reported functional measure. The relationships among mental health and self‐reported functioning should be considered when interpreting studies that use functional status questionnaires.
Aminobisphosphonate inhibition of interleukin‐1—induced bone resorption in mouse calvariae
Authors: Tan Paul L. J. , Katz June M. , Ames Ruth , Caughey David E. , Gray Harley D. , Ibbertson H. Kaye , Watson James D. 
Published: February 2007

AbstractInterleukin‐1 (IL‐1) is probably an important lymphokine mediator of inflammation and bone resorption. IL‐1 derived from mononuclear cells, a melanoma cell line (MM96 cells), and recombinant human IL‐1 (rHuIL‐1β) increased in vitro bone resorption, as measured by the release of 45Ca from cultured mouse calvariae. The 50% maximum active resorption was observed with 0.125 ng/ml or approximately 10−11M rHuIL‐1β. The resorptive action of IL‐1 was not entirely dependent on prostaglandin mediation, since its effect was evident when prostaglandin synthesis was inhibited in the cultures by indomethacin. IL‐1—induced resorption has been shown to be inhibited by 10−5M 3‐amino‐1‐hydroxypropylidene‐1‐1‐bisphosphonate (APD). This inhibition was partially reversed by increasing doses of IL‐1. In vitro toxicity studies showed that at concentrations of 10−4M, APD inhibited the growth of cultured MM96, murine myelomonocytic P388D1, and rat osteosarcoma UMR 106 cells, but not other mast and lymphoid cell lines. These in vitro observations may have relevance to the use of APD in bone and joint diseases in which inflammation and bone resorption are prominent.
Interleukin‐6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides
Authors: Houssiau Frédéric A. , Devogelaer Jean‐Pierre , Damme Jo Van , Deuxchaisnes Charles Nagant De , Snick Jacques Van 
Published: February 2007

AbstractInterleukin‐6 (IL‐6), also called 26‐kd protein, hybridoma plasmacytoma growth factor, β2‐interferon, or B cell stimulatory factor 2, is a recently described human cytokine with multiple growth and differentiation activities. Using a very sensitive bioassay based on the growth factor activity of this protein for B cell hybridomas, we found that IL‐6 activity was significantly elevated in synovial fluid from patients with rheumatoid arthritis (RA) or other inflammatory arthritides, as compared with that in a group of patients with osteoarthritis. Moreover, IL‐6 was detected in about one‐third of the serum samples from patients with RA. In the latter group, we found a significant correlation between serum IL‐6 activity and serum levels of C‐reactive protein, α1‐acid glycoprotein, α1‐antitrypsin, fibrinogen, and haptoglobin, which indicates that IL‐6 is related to disease activity in patients with RA.
Costochondral involvement in systemic candidiasis in heroin addicts: clinical, scintigraphic, and histologic features in 26 patients
Authors: Miro J. M. , Brancos M. A. , Abello R. , Lomena F. , Bisbe J. , Ribalta T. , Rotes‐Querol J. 
Published: February 2007

AbstractWe studied the clinical, scintigraphic, and histopathologic characteristics of 26 intravenous drug abusers with costochondral involvement secondary to systemic infection with Candida albicans. The clinical findings were of a mass appearing in the anterior region of the thorax. In general, signs of inflammation were absent. Histopathologic study of this costochondral mass in 12 patients showed perichondritis in 100% and myositis in 87%, with secondary involvement of cartilage in 43% and of bone in 75%. Results of bone scintigrams using 99mTc‐methylene diphosphonate were positive in only 7 of 15 patients (47%), with a correlation between positive uptake and osteitis. Gallium scintigraphy findings were positive in 9 of 10 patients (90%). The greater sensitivity of 67Ga was probably because the invariably present pericartilaginous inflammatory mass was not always accompanied by secondary cartilage and bone involvement.
Network regulation in rheumatoid arthritis. studies of dr+ t cells, anti‐dr, antiidiotypic antibodies, and clinical disease activity
Authors: Searles Robert P. , Savage Susan M. , Broz̈ek Celestyn M. , Marnell Lorraine L. , Hoffman Charles L. 
Published: February 2007

AbstractWe prospectively studied rheumatoid arthritis patients with various degrees of clinical disease activity, for the presence of DR+ T cells by flow cytometry, for anti‐DR using immunoblot analysis, and for antiidiotypic (anti‐id) antibodies by enzyme‐linked immunosorbent assay using F(ab′)2 monoclonal antibody anti‐DR L243 as idiotype. DR+ T cells correlated positively with anti‐DR, and anti‐id correlated negatively with both DR+ T cells and anti‐DR. Active clinical disease correlated positively with both DR+ T cells and anti‐DR, and correlated negatively with anti‐id. This DR antigen/anti‐DR/anti‐id network may control disease activity in rheumatoid arthritis patients.
The effects of leucovorin (folinic acid) on methotrexate therapy in rheumatoid arthritis patients
Authors: Tishler Moshe , Caspi Dan , Fishel Beno , Yaron Michael 
Published: February 2007

AbstractHigh‐dose leucovorin (folinic acid) supplementation was tested in a prospective, unblinded manner for 4 weeks in 7 rheumatoid arthritis patients who were being treated successfully with low‐dose methotrexate (MTX). Nausea caused by MTX disappeared; however, the underlying rheumatic disease worsened in all patients. Subjective clinical assessment, Ritchie articular index, grip strength, erythrocyte sedimentation rate, and levels of C‐reactive protein showed statistically significant deterioration. All these parameters improved after the leucovorin was stopped. This is the first direct clinical evidence implying folate antagonism in the action of low‐dose MTX therapy in rheumatoid arthritis patients.
Complement c4b–null alleles in felty's syndrome
Authors: Thomson Wendy , Sanders Paul A. , Davis Martin , Davidson Judith , Dyer Philip A. , Grennan David M. 
Published: February 2007

AbstractC4A and C4B allotypes were compared in 20 patients with Felty's syndrome (FS), 52 patients with rheumatoid arthritis (RA), and 55 control subjects. Nineteen of the FS patients had HLA–DR4. A C4B‐null allele was more frequent in the patients with FS (60%) than in either the RA patients (15%) or the control subjects (26%). Only the differences between patients with FS and those with RA remained statistically significant when DR4 positive subjects were compared. The C4B null allele may identify individuals within the rheumatoid population who are at risk of developing particular systemic complications.
Psoriatic arthritis and acquired immunodeficiency syndrome
Authors: Espinoza Luis R. , Berman Alberto , Vasey Frank B. , Cahalin Cay , Nelson Robert , Germain Bernard F. 
Published: February 2007

AbstractWe describe 2 patients who developed psoriatic arthritis during the course of acquired immunodeficiency syndrome. Both patients developed skin and articular involvement characteristic of psoriatic arthritis; these manifestations were refractory to conventional therapy. Our findings suggest that psoriatic arthritis should be added to the expanding spectrum of musculoskeletal manifestations of the acquired immunodeficiency syndrome.
Modulation of urokinase‐type plasminogen activator messenger rna levels in human synovial fibroblasts by interleukin‐1, retinoic acid, and a glucocorticoid
Authors: Vitti Gerard , Hamilton John A. 
Published: February 2007

AbstractPrevious studies have shown that mononuclear cell–conditioned medium (MCCM), interleukin‐1 (IL‐1), and all‐trans‐retinoic acid rapidly stimulate, while glucocorticoids lower, the urokinase‐type plasminogen activator (u‐PA) activity of human synovial fibroblastlike cells. It is now reported that MCCM, recombinant human IL‐1α (rHuIL‐1α), rHuIL‐1β, and all‐trans‐retinoic acid elevate the u‐PA messenger RNA (mRNA) levels to a steady‐state value within 2 hours, while dexamethasone (10−7M) inhibits this increase. For both situations, when the u‐PA activity is either stimulated or reduced, the changes in the u‐PA mRNA levels parallel the changes in the u‐PA activity, and it is suggested that modulation of gene transcription plays an important role.
Demonstration and characterization of a transient arthritis in rats following sensitization of synovial mast cells with antigen‐specific ige and parenteral challenge with specific antigen
Authors: Malone Daniel G. , Metcalfe Dean D. 
Published: February 2007

AbstractWe have developed a model of IgE‐dependent, mast cell–mediated arthritis in rats. One knee joint (test joint) of a Sprague‐Dawley rat was injected with 1 μg of a monoclonal IgE specific for dinitrophenol, and the contralateral (control) joint was injected with the same amount of an irrelevant monoclonal IgE in phosphate buffered saline or with phosphate buffered saline alone. Within 5 minutes of intravenous injection of antigen, an acute, transient arthritis occurred in the test joints only, with swelling and extravasation of intravascular blue dye and 125I‐labeled albumin, decreased numbers of stainable mast cells, and decreased histamine content of the joint synovium. Pretreatment of experimental animals with H1 and H2 antihistamines did not completely block the reaction. These data show that IgE‐dependent synovial mast cell degranulation causes a transient, nondestructive arthritis, reminiscent of lupus arthritis and intermittent hydrarthrosis.