bluuebunny/crossref_metadata_2025_split huggingface dataset:
Violence In the Classroom
Authors: Bender William N. , McLaughlin Phillip J. 
Published: December 2008

The authors provide an overview of this special issue on preparing administrators and teachers to handle violence and problematic behaviors in schools This issue of Intervention in School and Clinic focuses on the topic of violence in the classroom. In addition to acquainting our readers with this area, the various articles also present curriculum and teaching models that can be used in integrated settings. I am pleased that Professors William N. Bender and Phillip J. McLaughlin, who have assembled an outstanding blend of both authors and topics, agreed to serve as guest editors of this issue. We are honored that Intervention is able to showcase the outstanding work of so many talented individuals. We hope you enjoy reading the articles, and we welcome your comments.—GW
Nutrient Intake and Obesity in Children with Autism
Authors: Ho Helena H. , Eaves Linda C. , Peabody Diana 
Published: December 2008

Many children with autism have idiosyncratic food preferences and eating habits that are difficult to regulate. The nutrient intake of 54 school-age Canadian children with autistic spectrum disorders, at mean age 13.3 years, was assessed using 3-day food consumption diaries compiled by parents or caregivers. The food records were analyzed for energy, minerals, vitamins, carbohydrates, protein, and fat content and compared with the recommended nutrient intake (RNI) for Canadians. All children had adequate protein intake, but only the diets of 4 children (7.4%) met the recommended servings from each food group recommended by the Canadian Food Guide. Twenty children (37%) with adequate vitamin intake received additional vitamin supplement, and 18 children (33%) not supplemented were low in calcium intake. The entire group consumed more carbohydrates and less fat than the average Canadian child but had similar calorie intake. Twenty-three children (42.6%) were found to be obese (to have greater than 120% of ideal body weight), but only five of these were overeating. Nearly half of the obese children had a low level of activity. Obesity was found to be correlated positively with severity of autism but not with gender, parents' educational levels, intake of various food groups, or with overeating alone.
A metal halide high pressure discharge lamp with warm white colour and high efficacy
Authors: Fromm D.C. , Seehawer J. , Wagner W.-J. 
Published: December 2008

A Na, Tl, In, Sn high pressure discharge lamp of warm white colour was developed for an efficient energy utilization in indoor illumination. The lamp is characterised by: (1) low colour temperature (CCT = 3300 K) with good colour rendering (Ra = 85). (2) high luminous efficacy of approx. 90 lm/W, corresponding to the 5- to 6-fold of an incandescent lamp, (3) high life time of approx. 4000 h, corresponding to the 4-fold of an incandescent lamp, (4) arbitrary burning position as with an incandescent lamp. These data were achieved through utilization of strongly pressure broadened line radiation (Na, Tl, In) combined with molecular radiation (SnI, SnBr, HgI, HgBr). The main features of the lamp are discussed, in particular a deviation from local thermal equilibrium in the mantle of the discharge.
Movement under emergency lighting: the effect of illuminance
Authors: Boyce P.R. 
Published: December 2008

This paper describes an investigation carried out in a large open plan office with the aim of identifying the illuminance necessary for rapid, safe movement under emergency lighting conditions. In the experiment, the times taken for each subject to cover an escape route were measured. The manner in which the subjects moved was observed and their opinions of the conditions were noted. This data was collected from sixty subjects in all, twelve at each illuminance condition. The subjects were not familiar with the office and were of working age. From the data collected it is concluded that at a mean illuminance of 0.2 lx, people experience some difficulty in moving quickly and unhesitatingly over the escape route. At a mean illuminance of 1 Ix there is no difficulty in moving smoothly and steadily over the escape route at a speed similar to that achieved under the normal room lighting. These results suggest that the present emergency lighting criterion of a minimum illuminance of 0.2 Ix is only reasonable if it is indeed treated as an absolute minimum. They also indicate that it would be useful to adopt a mean illuminance of 1 Ix on the escape route as an additional emergency lighting criterion. Three subsidiary experiments were carried out to explore the effects of age, familiarity with the office and group movement rather than individual movement. These subsidiary experiments showed that these factors made little difference to the subject's ability to move under the emergency lighting. Therefore the conclusions reached for the main experiment also apply to older people of working age, to those who are familiar with the space and to people moving in small groups.
Levels of Visual Abstraction and the Assessment of Language Comprehension in Trainable Mentally Retarded Children
Authors: Woznak Diana , Lasky Elaine Z. 
Published: December 2008

Assessing language comprehension is critical for developing intervention programs for children who are mentally retarded. Since children's visual perceptual development affects how they attend to, interpret, and respond to stimuli, their performance may be affected by varying the stimuli. More concrete, more redundant stimuli may alter the performance. In this study, language comprehension performance scores of each TMR child improved in response to more concrete co/or photographs as compared to more abstract line drawings. Implications for diagnosis and intervention are discussed.
Hidden abnormalities and novel classification of t(15;17) acute promyelocytic leukemia (APL) based on genomic alterations
Authors: Akagi Tadayuki , Shih Lee-Yung , Kato Motohiro , Kawamata Norihiko , Yamamoto Go , Sanada Masashi , Okamoto Ryoko , Miller Carl W. , Liang Der-Cherng , Ogawa Seishi , Koeffler H. Phillip 
Published: December 2008

AbstractAcute promyelocytic leukemia (APL) is a hematopoietic malignant disease characterized by the chromosomal translocation t(15;17), resulting in the formation of the PML-RARA gene. Here, 47 t(15;17) APL samples were analyzed with high-density single-nucleotide polymorphism microarray (50-K and 250-K SNP-chips) using the new algorithm AsCNAR (allele-specific copy-number analysis using anonymous references). Copy-number-neutral loss of heterozygosity (CNN-LOH) was identified at chromosomes 10q (3 cases), 11p (3 cases), and 19q (1 case). Twenty-eight samples (60%) did not have an obvious alteration (normal-copy-number [NC] group). Nineteen samples (40%) showed either one or more genomic abnormalities: 8 samples (17%) had trisomy 8 either with or without an additional duplication, deletion, or CNN-LOH (+8 group); and 11 samples (23%) had genomic abnormalities without trisomy 8 (other abnormalities group). These chromosomal abnormalities were acquired somatic mutations. Interestingly, FLT3-ITD mutations (11/47 cases) occurred only in the group with no genomic alteration (NC group). Taken together, these results suggest that the pathway of development of APL differs in each group: FLT3-ITD, trisomy 8, and other genomic changes. Here, we showed for the first time hidden abnormalities and novel disease-related genomic changes in t(15;17) APL.
Transplantation of allogeneic T cells alters iron homeostasis in NOD/SCID mice
Authors: Bair Steven , Spaulding Emily , Parkkinen Jaakko , Shulman Howard M. , Lesnikov Vladimir , Beauchamp Mary , Canonne-Hergaux François , Kowdley Kris V. , Deeg H. Joachim 
Published: December 2008

AbstractIron overload is common in patients undergoing allogeneic hematopoietic cell transplantation (HCT), but the mechanisms leading to overload are unknown. Here, we determined iron levels and the expression of iron regulatory proteins in the liver and gut of nonobese diabetic–severe combined immunodeficient (NOD/SCID) mice that underwent transplantation with syngeneic (histocompatible) or allogeneic (histoincompatible) T lymphocytes. Infusion of histoincompatible T cells resulted in a significant rise in serum iron levels and liver iron content. Iron deposition was accompanied by hepatocyte injury and intestinal villous damage. Feeding of low- or high-iron diet was associated with appropriate ferroportin 1 and hepcidin responses in mice given histocompatible T cells, whereas mice given histoincompatible T cells showed inappropriate up-regulation of duodenal ferroportin 1 and a loss of expression of hepatic hepcidin. These findings suggest that alloreactive T cell–dependent signals induced dysregulation of intestinal iron absorption, which contributed to liver iron overload after HCT.
Mucosal immune responses to HIV-1 in elite controllers: a potential correlate of immune control
Authors: Ferre April L. , Hunt Peter W. , Critchfield J. William , Young Delandy H. , Morris Megan M. , Garcia Juan C. , Pollard Richard B. , Yee Hal F. , Martin Jeffrey N. , Deeks Steven G. , Shacklett Barbara L. 
Published: December 2008

AbstractThere exists a unique group of persons who are able to durably control HIV in the absence of therapy. The mechanisms of control in these persons remain poorly defined. In this study, we examined CD8+ T-cell responses in blood and rectal mucosa from 17 “elite controllers” (viral load < 75 copies/mL), 11 “viremic controllers” (75-2000 copies/mL), 14 noncontrollers (> 10 000 copies/mL), and 10 antiretroviral-treated persons (< 75 copies/mL). Production of interferon-γ, interleukin-2, tumor necrosis factor-α, macrophage inflammatory protein-1β, and CD107a by CD8+ T cells in response to HIV-1 Gag stimulation was measured using flow cytometry. Our hypothesis was that “polyfunctional” T cells producing multiple antiviral factors would be most abundant in mucosal tissues of HIV controllers. Mucosal CD8+ T-cell responses were significantly stronger and more complex in controllers than in antiretroviral-suppressed persons (P = .0004). The frequency of 4-function responses in rectal mucosa was higher in controllers than in noncontrollers and patients on therapy (P < .0001). Mucosal responses in controllers were frequently stronger and more complex than blood responses. These findings demonstrate that many controllers mount strong, complex HIV-specific T-cell responses in rectal mucosa. These responses may play an important role in mucosal immune surveillance, as suggested by their relative enrichment among persons who control HIV in the absence of therapy.
NOTCH1/FBXW7 mutation identifies a large subgroup with favorable outcome in adult T-cell acute lymphoblastic leukemia (T-ALL): a Group for Research on Adult Acute Lymphoblastic Leukemia (GRAALL) study
Authors: Asnafi Vahid , Buzyn Agnès , Le Noir Sandrine , Baleydier Frédéric , Simon Arnauld , Beldjord Kheira , Reman Oumedaly , Witz Francis , Fagot Thierry , Tavernier Emmanuelle , Turlure Pascal , Leguay Thibaut , Huguet Françoise , Vernant Jean-Paul , Daniel Francis , Béné Marie-Christine , Ifrah Norbert , Thomas Xavier , Dombret Hervé , Macintyre Elizabeth 
Published: December 2008

Abstract
Many somatic genetic abnormalities have been identified in T-cell acute lymphoblastic leukemia (T-ALL) but each individual abnormality accounts for a small proportion of cases; therapeutic stratification consequently still relies on classical clinical markers. NOTCH1 and/or FBXW7 mutations both lead to activation of the NOTCH1 pathway and are among the most frequent mutations in T-ALL. We screened 141 adult diagnostic T-ALL samples from patients treated on either the Lymphoblastic Acute Leukemia in Adults (LALA)-94 (n = 87) or the GRAALL-2003 (n = 54) trials. In 88 cases (62%) there were demonstrated NOTCH1 mutations (42% heterodimerization [HD], 10% HD+proline glutamate serine threonine [PEST], 6% PEST, 2% juxtamembrane mutations, 2% transactivation domain [TAD]) and 34 cases (24%) had FBXW7 mutations (21 cases had both NOTCH1 and FBXW7 mutations); 40 cases (28%) were wild type for both. There was no significant correlation between NOTCH1 and/or FBXW7 mutations and clinico-biologic features. Median event-free survival (EFS) and overall survival (OS) were 36 versus 17 months (P = .01) and not reached versus 32 months (P = .004) in patients with NOTCH1 and/or FBXW7 mutations versus other patients, respectively. Multivariate analysis showed that the presence of NOTCH1/FBXW7 mutations was an independent good prognostic factor for EFS and OS (P = .02 and P = .01, respectively). These data demonstrate that NOTCH1 pathway activation by either NOTCH1 or FBXW7 mutation identifies a large group of patients with a favorable outcome that could justify individual therapeutic stratification for T-ALL.
Subcellular localization of ammonium transporters in Dictyostelium discoideum
Authors: Kirsten Janet H , Xiong Yanhua , Davis Carter T , Singleton Charles K 
Published: December 2008

Abstract

Background
With the exception of vertebrates, most organisms have plasma membrane associated ammonium transporters which primarily serve to import a source of nitrogen for nutritional purposes. Dictyostelium discoideum has three ammonium transporters, Amts A, B and C. Our present work used fluorescent fusion proteins to determine the cellular localization of the Amts and tested the hypothesis that the transporters mediate removal of ammonia generated endogenously from the elevated protein catabolism common to many protists.

Results
Using RFP and YFP fusion constructs driven by the actin 15 promoter, we found that the three ammonium transporters were localized on the plasma membrane and on the membranes of subcellular organelles. AmtA and AmtB were localized on the membranes of endolysosomes and phagosomes, with AmtB further localized on the membranes of contractile vacuoles. AmtC also was localized on subcellular organelles when it was stabilized by coexpression with either the AmtA or AmtB fusion transporter. The three ammonium transporters exported ammonia linearly with regard to time during the first 18 hours of the developmental program as revealed by reduced export in the null strains. The fluorescently tagged transporters rescued export when expressed in the null strains, and thus they were functional transporters.

Conclusion
Unlike ammonium transporters in most organisms, which import NH3/NH4
+ as a nitrogen source, those of Dictyostelium export ammonia/ammonium as a waste product from extensive catabolism of exogenously derived and endogenous proteins. Localization on proteolytic organelles and on the neutral contractile vacuole suggests that Dictyostelium ammonium transporters may have unique subcellular functions and play a role in the maintenance of intracellular ammonium distribution. A lack of correlation between the null strain phenotypes and ammonia excretion properties of the ammonium transporters suggests that it is not the excretion function that is important for coupling ammonia levels to the slug versus culmination choice, but rather a sensor and/or signaling function of these proteins that is important.
A large-scale survey of genetic copy number variations among Han Chinese residing in Taiwan
Authors: Lin Chien-Hsing , Li Ling-Hui , Ho Sheng-Feng , Chuang Tzu-Po , Wu Jer-Yuarn , Chen Yuan-Tsong , Fann Cathy SJ 
Published: December 2008

Abstract

Background
Copy number variations (CNVs) have recently been recognized as important structural variations in the human genome. CNVs can affect gene expression and thus may contribute to phenotypic differences. The copy number inferring tool (CNIT) is an effective hidden Markov model-based algorithm for estimating allele-specific copy number and predicting chromosomal alterations from single nucleotide polymorphism microarrays. The CNIT algorithm, which was constructed using data from 270 HapMap multi-ethnic individuals, was applied to identify CNVs from 300 unrelated Han Chinese individuals in Taiwan.

Results
Using stringent selection criteria, 230 regions with variable copy numbers were identified in the Han Chinese population; 133 (57.83%) had been reported previously, 64 displayed greater than 1% CNV allele frequency. The average size of the CNV regions was 322 kb (ranging from 1.48 kb to 5.68 Mb) and covered a total of 2.47% of the human genome. A total of 196 of the CNV regions were simple deletions and 27 were simple amplifications. There were 449 genes and 5 microRNAs within these CNV regions; some of these genes are known to be associated with diseases.

Conclusion
The identified CNVs are characteristic of the Han Chinese population and should be considered when genetic studies are conducted. The CNV distribution in the human genome is still poorly characterized, and there is much diversity among different ethnic populations.
Meta-analysis of expression signatures of muscle atrophy: gene interaction networks in early and late stages
Authors: Calura Enrica , Cagnin Stefano , Raffaello Anna , Laveder Paolo , Lanfranchi Gerolamo , Romualdi Chiara 
Published: December 2008

Abstract

Background
Skeletal muscle mass can be markedly reduced through a process called atrophy, as a consequence of many diseases or critical physiological and environmental situations. Atrophy is characterised by loss of contractile proteins and reduction of fiber volume. Although in the last decade the molecular aspects underlying muscle atrophy have received increased attention, the fine mechanisms controlling muscle degeneration are still incomplete. In this study we applied meta-analysis on gene expression signatures pertaining to different types of muscle atrophy for the identification of novel key regulatory signals implicated in these degenerative processes.

Results
We found a general down-regulation of genes involved in energy production and carbohydrate metabolism and up-regulation of genes for protein degradation and catabolism. Six functional pathways occupy central positions in the molecular network obtained by the integration of atrophy transcriptome and molecular interaction data. They are TGF-β pathway, apoptosis, membrane trafficking/cytoskeleton organization, NFKB pathways, inflammation and reorganization of the extracellular matrix. Protein degradation pathway is evident only in the network specific for muscle short-term response to atrophy. TGF-β pathway plays a central role with proteins SMAD3/4, MYC, MAX and CDKN1A in the general network, and JUN, MYC, GNB2L1/RACK1 in the short-term muscle response network.

Conclusion
Our study offers a general overview of the molecular pathways and cellular processes regulating the establishment and maintenance of atrophic state in skeletal muscle, showing also how the different pathways are interconnected. This analysis identifies novel key factors that could be further investigated as potential targets for the development of therapeutic treatments. We suggest that the transcription factors SMAD3/4, GNB2L1/RACK1, MYC, MAX and JUN, whose functions have been extensively studied in tumours but only marginally in muscle, appear instead to play important roles in regulating muscle response to atrophy.
Forced IFIT-2 expression represses LPS induced TNF-alpha expression at posttranscriptional levels
Authors: Berchtold Susanne , Manncke Birgit , Klenk Juliane , Geisel Julia , Autenrieth Ingo B , Bohn Erwin 
Published: December 2008

AbstractBackgroundInterferon induced tetratricopeptide repeat protein 2 (IFIT-2, P54) belongs to the type I interferon response genes and is highly induced after stimulation with LPS. The biological function of this protein is so far unclear. Previous studies indicated that IFIT-2 binds to the initiation factor subunit eIF-3c, affects translation initiation and inhibits protein synthesis. The aim of the study was to further characterize the function of IFIT-2.ResultsStimulation of RAW264.7 macrophages with LPS or IFN-γ leads to the expression of IFIT-2 in a type I interferon dependent manner. By using stably transfected RAW264.7 macrophages overexpressing IFIT-2 we found that IFIT-2 inhibits selectively LPS induced expression of TNF-α, IL-6, and MIP-2 but not of IFIT-1 or EGR-1. In IFIT-2 overexpressing cells TNF-α mRNA expression was lower after LPS stimulation due to reduced mRNA stability. Further experiments suggest that characteristics of the 3'UTR of transcripts discriminate whether IFIT-2 has a strong impact on protein expression or not.ConclusionOur data suggest that IFIT-2 may affect selectively LPS induced protein expression probably by regulation at different posttranscriptional levels.
Histone H1x is highly expressed in human neuroendocrine cells and tumours
Authors: Warneboldt Julia , Haller Florian , Horstmann Olaf , Danner Bernhard C , Füzesi László , Doenecke Detlef , Happel Nicole 
Published: December 2008

AbstractBackgroundHistone H1x is a ubiquitously expressed member of the H1 histone family. H1 histones, also called linker histones, stabilize compact, higher order structures of chromatin. In addition to their role as structural proteins, they actively regulate gene expression and participate in chromatin-based processes like DNA replication and repair. The epigenetic contribution of H1 histones to these mechanisms makes it conceivable that they also take part in malignant transformation.MethodsBased on results of a Blast data base search which revealed an accumulation of expressed sequence tags (ESTs) of H1x in libraries from neuroendocrine tumours (NETs), we evaluated the expression of H1x in NETs from lung and the gastrointestinal tract using immunohistochemisty. Relative protein and mRNA levels of H1x were analysed by Western blot analysis and quantitative real-time RT-PCR, respectively. Since several reports describe a change of the expression level of the replacement subtype H1.0 during tumourigenesis, the analysis of this subtype was included in this study.ResultsWe found an increased expression of H1x but not of H1.0 in NET tissues in comparison to corresponding normal tissues. Even though the analysed NETs were heterogenous regarding their grade of malignancy, all except one showed a considerably higher protein amount of H1x compared with corresponding non-neoplastic tissue. Furthermore, double-labelling of H1x and chromogranin A in sections of pancreas and small intestine revealed that H1x is highly expressed in neuroendocrine cells of these tissues.ConclusionWe conclude that the high expression of histone H1x in NETs is probably due to the abundance of this protein in the cells from which these tumours originate.