bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nonconvulsive Seizures in the Pediatric Intensive Care Unit: Etiology, EEG, and Brain Imaging Findings
Authors: Saengpattrachai Montri , Sharma Rohit , Hunjan Amrita , Shroff Manohar , Ochi Ayako , Otsubo Hiroshi , Cortez Miguel A. , Carter Snead O. 
Published: August 2006

Summary:  Purposes: To determine the occurrence of nonconvulsive seizures (NCS) in the Pediatric Intensive Care Unit (PICU); to ascertain the relationship of NCS to past medical history, etiology, EEG, and brain imaging; and to determine the concordance between abnormal EEG findings and neuroimaging abnormalities. Methods: A retrospective review was conducted of all pediatric patients who were admitted or transferred to the PICU from January 2000 to December 2003 with an unexplained decrease in level of consciousness, no overt clinical seizures, and EEG recordings performed within the 24 h of onset of an altered state of consciousness. Results: Twenty‐three of 141 patients who met criteria for inclusion in the study (16.3%) were found to have NCS. The male to female ratio was 1.9:1. The largest group of patients (43%) had no preexisting neurological condition prior to the onset of NCS. In the remainder, the etiology of NCS included: acute structural brain lesion (48%), acute nonstructural brain lesion (22%), epilepsy‐related seizure (13%), and others (17%). Epileptic foci were lateralized to the right side in 39.2%, the left side in 30.4%, and were bilateral in 30.4%. Of 23 patients with NCS, 18 (78.3%) demonstrated abnormal neuroimaging. In 10 of 18 of these patients (55.6%), the findings on neuroimaging were concordant with the lateralization found on EEG (p < 0.05, Fisher's exact test). Conclusions: NCS are not uncommon in pediatric patients with an altered state of consciousness. Almost half of the patients were previously healthy especially if they were under 6 months of age. This report highlights the importance of clinical awareness of NCS in the PICU.
The BOLD Effect of Interictal Spike Activity in Childhood Occipital Lobe Epilepsy
Authors: Leal Alberto , Dias Ana , Vieira José P , Secca Mário , Jordão Constança 
Published: August 2006

Summary:  Purpose: Occipital lobe epilepsy (OLE) presents in childhood with different manifestations, age of onset and EEG features that form distinct syndromes. The ictal clinical symptoms are difficult to correlate with onset in particular areas in the occipital lobes, and the EEG recordings have not been able to overcome this limitation. The mapping of epileptogenic cortical regions in OLE remains therefore an important goal in our understanding of these syndromes. Methods: In this work, three patients with two types of idiopathic childhood OLE were studied with EEG source analysis and also with mapping of the BOLD effect associated with spikes in simultaneous EEG/fMRI recordings. Results: Two patients with late onset OLE provided EEG source localizations in the lateral parietal cortex and in the medial occipital areas. The BOLD activations were more consistent and restricted to the medial parietal‐occipital cortex in both cases. One patient with photosensitive idiopathic OLE presented with dipole sources in the medial parietal cortex, but the BOLD activations were widespread over inferior and bilateral occipital areas and also posterior temporal ones. There was little spatial overlap between the EEG and BOLD results, but the localizations suggested by the latter are more consistent with the ictal clinical manifestations of each type of epileptic syndrome. Conclusions: Overall, the BOLD effect associated with interictal spikes maps epileptogenic areas to different localizations than the ones suggested by EEG source analysis. These maps are similar in two patients with late onset idiopathic OLE, but different from a case of photosensitive idiopathic OLE.
Debris retention and wear in three different nickel‐titanium rotary instruments
Authors: Elmsallati Elham A. , Wadachi Reiko , Ebrahim Aqeel Khalil , Suda Hideaki 
Published: August 2006

AbstractThe aim of this in vitro study was to compare three nickel‐titanium (Ni‐Ti) rotary instruments: EndoWave, K3 and ProFile, with respect to their debris retention and wear. Thirty simulated root canal models made from resin blocks with 40° canal curvature were used. They were divided into three groups of 10 samples each; EndoWave, K3 and ProFile were used in Groups A, B and C respectively. They were prepared with four serial size files. The weight of each file was measured to evaluate debris retention and wear at four representative time points: prior to root canal preparation (W1), immediately after root canal preparation (W2), after ultrasonic cleaning (W3), after further additional cleaning (W4). The weight of debris on each file after root canal instrumentation and ultrasonic cleaning was calculated as D1: (W2 – W4) and D2: (W3 – W4) respectively. Also, wear of the file was calculated as WF: (W1 – W4). There was a significant difference in the amount of debris remaining on the file among the three files; EndoWave retained more debris (D1) than K3 and ProFile (P < 0.001, one‐way anova). ProFile had the largest amount of debris (D2) remaining after ultrasonic cleaning (P < 0.001). Wear was not detectable in this study. Under the conditions of this study, immediately after root canal preparation, EndoWave retained the largest amount of debris, while ProFile retained the most debris even after ultrasonic cleaning.
Evaluation of the antibacterial substantivity of several intra‐canal agents
Authors: Khademi Abbas ali , Mohammadi Zahed , Havaee Asgar 
Published: August 2006

AbstractThe aim of this in vitro study was to compare the antimicrobial substantivity of 2% chlorhexidine gluconate (CHX), 100 mg ml−1 doxycycline and 2.6% sodium hypochlorite (NaOCl) in bovine root dentine. Eighty dentine tubes prepared from bovine incisors were infected in vitro for 14 days with Enterococcus faecalis. The specimens were divided into five groups as follows: doxycycline HCl; CHX; NaOCl; infected dentine tubes (positive control); and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth. After culturing, the number of colony‐forming units (CFU) was counted. In all experimental groups, the number of CFU was minimum in the first cultures, and the results obtained were significantly different from each other at any time period (P < 0.05). In the first culture, the NaOCl group and doxycycline HCl group showed the lowest and highest number of CFU, respectively. In each group, the number of CFU increased significantly by time‐lapse (P < 0.05). In conclusion, the substantivity of CHX was significantly greater than NaOCl and doxycycline.
In vitro computer analysis of crown discolouration from commonly used endodontic sealers
Authors: Partovi Mandana , Al‐Havvaz Abdol H. , Soleimani Banafsheh 
Published: August 2006

AbstractThe aim of this study was to assess the degree of staining of tooth crowns by commonly used endodontic sealers using a computer analysis method. Crown discolouration by root canal sealers AH26, Endofill, Tubliseal, Zinc oxide eugenol (ZnOE), Apatite root canal sealer III as well as gutta‐percha and Cavizol (a filling material containing ZnOE) were tested on extracted human premolar teeth. The roots of the teeth were resected 3 mm below the cemento‐enamel junction. The pulp chambers were then cleaned and irrigated. The samples were divided into nine groups of five samples each and filled with test materials. Ten teeth were used as control groups: five positive (amalgam) and five negative (distilled water). The degree of tooth discolouration was analysed at 3, 6 and 9 months. The crown discolouration was assessed by computer analysis of digital images taken from the samples using the CIE Lab colour system. Statistical analysis was carried out using anova, repeated measure anova and Tukey’s HSD tests. All sealers caused a degree of tooth discolouration, which increased with time. Endofill and ZnOE caused the greatest discolouration and Apatite root canal sealer III caused the least discolouration after 9 months. The most discolouration during the test periods occurred in the cervical third of the crown.
Multiprobe fluorescence in situ hybridisation: prognostic perspectives in superficial bladder cancer
Authors: Mian C , Lodde M , Comploj E , Lusuardi L , Palermo S , Mian M , Maier K , Pycha A 
Published: August 2006

Aim:To establish independent prognostic factors on a chromosomal basis in superficial bladder cancer, using a multicolour fluorescence in situ hybridisation (FISH) probe mix.Patients and methods:In 2002, voided urine from 75 consecutive patients (mean age 71.7, range 52–93) years under follow-up for superficial urothelial cancer was studied prospectively. The patients were observed for a mean (standard deviation (SD)) period of 39.3 (6.8) months (range 27–58) until July 2005. A multicolour FISH on liquid-based voided urinary cytology was carried out on all patients. Univariate analysis, using a log rank test, was used to determine the prognostic relevance of a low-risk pattern and a high-risk pattern. Progression-free survival time was calculated from the date of first diagnosis to first recurrence or progression according to the Kaplan–Meier product-limit method.Results:One patient was lost to follow-up. 27 of the 74 remaining (36.8%) patients showed recurrent disease. In 9 (33.3%) patients with a low-risk pattern disease recurred after a mean (SD) observation time of 29.7 (1.9) months (range 8.3–52.3, median 30.8 (12.4)). 18 (66.7%) patients with a high-risk pattern developed recurrence within a mean (SD) of 17.6 (2.0) months (range 4–38.8, median 16.7 (11.6)). The Kaplan–Meier curve for progression-free survival showed marked differences between the low-risk and the high-risk groups.Conclusion:Patients with a high-risk chromosomal pattern have a markedly shorter disease-free survival time and higher progression rate than patients with a low-risk pattern. High-risk patients can therefore be treated more aggressively to prevent tumour spreading.
Prevalence, intensity, and geographic distribution of sinus worm (Skrjabingylus nasicola) infection in mink (Mustela vison) of central Ontario
Authors: Santi Stacey A. , Parker Glenn H. , Schaffner Natalie P. , Capodagli Liane , Persinger Michael A. 
Published: August 2006

Mink (Mustela vison Schreber, 1777) skulls (n = 630) from 18 geographic regions throughout central Ontario were examined for levels of sinus worm (Skrjabingylus nasicola (Leuckart, 1842)) infection by exposing and counting adult worms present in each sinus cavity. Site differences in both infection prevalence (range 55.6%–100%) and infection intensity (2.4 ± 0.3 to 16.1 ± 2.2 worms per infected animal; mean ± SE) were found, but neither latitudinal nor longitudinal trends were apparent. Temporal differences in infection prevalence were not observed among selected regions sampled over two and three consecutive trapping seasons; however, at two of the three sites monitored, infection intensities increased approximately twofold. Juvenile animals of either sex indicated elevated levels of infection prevalence relative to adult males, while juvenile males, but not females, indicated significantly higher infection intensities than those seen in the adult male cohort. No bias toward left or right sinus location was noted in either prevalence or intensity of infections. Comparison of age- and sex-adjusted body mass failed to provide evidence for reduced physical condition in infected animals.
Is winter diet quality related to body condition of white-tailed deer (Odocoileus virginianus)? An experiment using urine profiles
Authors: Sauvé Daniel G. , Côté Steeve D. 
Published: August 2006

During winter, boreal forest herbivores have access to only poor-quality forage. On Anticosti Island (Quebec, Canada), the ongoing reduction of balsam fir (Abies balsamea (L.) P. Mill.) owing to overbrowsing by white-tailed deer (Odocoileus virginianus (Zimmermann, 1780)) may force deer to include a higher proportion of white spruce (Picea glauca (Moench) Voss), a browse normally avoided, in their winter diet. We tested the hypotheses that (i) deer body condition during winter and (ii) the costs of detoxification of plant secondary metabolites in the winter diet could be estimated by monitoring the 3-methylhistidine / creatinine and glucuronic acid / creatinine ratios, respectively, in urine collected in snow from white-tailed deer fawns. Doubling the amount of white spruce in the winter diet of deer (from the current 20% under natural conditions to 40%) did not increase 3-methylhistidine / creatinine ratios but increased the glucuronic acid / creatinine ratio in urine, suggesting that a diet containing more spruce was more toxic. A weak positive relationship was observed between 3-methylhistidine and percent cumulative mass loss. There was no relationship between the 3-methylhistidine / creatinine ratio and the number of days left before death, as well as no relationship between the ratio of glucuronic acid / creatinine and percent cumulative mass loss. We conclude that the costs of detoxification of plant secondary metabolites in the winter diet of white-tailed deer in boreal forests could be monitored with glucuronic acid / creatinine ratios, but that 3-methylhistidine / creatinine ratios were weak indicators of deer body condition in winter.
Expression of tumor-associated antigens in acute myeloid leukemia: implications for specific immunotherapeutic approaches
Authors: Greiner Jochen , Schmitt Michael , Li Li , Giannopoulos Krzysztof , Bosch Katrin , Schmitt Anita , Dohner Konstanze , Schlenk Richard F. , Pollack Jonathan R. , Dohner Hartmut , Bullinger Lars 
Published: August 2006

AbstractThe expression of tumor-associated antigens (TAAs) might play a critical role in the control of minimal residual disease (MRD) in acute myeloid leukemia (AML), and therefore might be associated with clinical outcome in AML. In a DNA microarray analysis of 116 AML samples, we found a significant correlation between high mRNA levels of G250/CA9 and longer overall survival (P = .022), a similar trend with high mRNA levels of PRAME (P = .103), and a hint for RHAMM/HMMR. In contrast, for other TAAs like WT1, TERT, PRTN3, BCL2, and LAMR1, we found no correlation with clinical outcome. High expression of at least 1 of the 3 TAAs, RHAMM/HMMR, PRAME, or G250/CA9, provided the strongest favorable prognostic effect (P = .005). Specific T-cell responses were detected in 8 (47%) of 17 patients with AML in complete remission for RHAMM/HMMR-R3 peptide, in 7 (70%) of 10 for PRAME-P3 peptide, and in 6 (60%) of 10 for newly characterized G250/CA9-G2 peptide, a significant increased immune response compared with patients with AML patients who had refractory disease (P < .001). Furthermore, we could demonstrate specific lysis of T2 cells presenting these epitope peptides. In conclusion, expression of the TAAs RHAMM/HMMR, PRAME, and G250/CA9 can induce strong antileukemic immune responses, possibly enabling MRD control. Thus, these TAAs represent interesting targets for polyvalent immunotherapeutic approaches in AML.
BCR ubiquitination controls BCR-mediated antigen processing and presentation
Authors: Drake Lisa , McGovern-Brindisi Erica M. , Drake James R. 
Published: August 2006

AbstractBCR-mediated antigen processing occurs at immunologically relevant antigen concentrations and hinges on the trafficking of antigen-BCR (Ag-BCR) complexes to class II–containing multivesicular bodies (MVBs) termed MIICs. However, the molecular mechanism underlying the trafficking of Ag-BCR complexes to and within MIICs is not well understood. In contrast, the trafficking of the epidermal growth factor receptor (EGFR) to and within MVBs occurs via a well-characterized ubiquitin-dependent mechanism, which is blocked by acute inhibition of proteasome activity. Using a highly characterized antigen-specific model system, it was determined that the immunoglobulin heavy chain subunit of the IgM BCR of normal (ie, nontransformed) B cells is ubiquitinated. Moreover, acute inhibition of proteasome activity delays the formation of ubiquitinated ligand–BCR complexes, alters the intracellular trafficking of internalized Ag-BCR complexes, and selectively blocks the BCR-mediated processing and presentation of cognate antigen, without inhibiting the endocytosis, processing, and presentation of non–cognate antigen internalized by fluidphase endocytosis. These results demonstrate that the trafficking of Ag-BCR complexes to and within MVB-like antigen processing compartments occurs via a molecular mechanism with similarities to that used by the EGFR, and establishes the EGFR as a paradigm for the further analysis of Ag-BCR trafficking to and within MIICs.
Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy
Authors: Schmitt E , Gehrmann M , Brunet M , Multhoff G , Garrido C 
Published: August 2006

AbstractStress or heat shock proteins (HSPs) are the most conserved proteins present in both prokaryotes and eukaryotes. Their expression is induced in response to a wide variety of physiological and environmental insults. These proteins play an essential role as molecular chaperones by assisting the correct folding of nascent and sress-accumulated misfolded proteins, and preventing their aggregation. HSPs have a dual function depending on their intracellular or extracellular location. Intracellular HSPs have a protective function. They allow the cells to survive lethal conditions. Various mechanisms have been proposed to account for the cytoprotective functions of HSPs. Several HSPs have also been demonstrated to directly interact with various components of the tightly regulated programmed cell death machinery, upstream and downstream of the mitochondrial events. On the other hand, extracellular located or membrane-bound HSPs mediate immunological functions. They can elicit an immune response modulated either by the adaptive or innate immune system. This review will focus on HSP27, HSP70, and HSP90. We will discuss the dual role of these HSPs, protective vs. immunogenic properties, making a special emphasis in their utility as targets in cancer therapy.
Mechanisms underlying activity of antiretroviral drugs in HIV-1-infected macrophages: new therapeutic strategies
Authors: Aquaro Stefano , Svicher Valentina , Schols Dominique , Pollicita Michela , Antinori Andrea , Balzarini Jan , Perno Carlo Federico 
Published: August 2006

Abstract
Monocyte-derived macrophages (M/M) are considered the second cellular target of HIV-1 and a crucial virus reservoir. M/M are widely distributed in all tissues and organs, including the CNS, where they represent the most common HIV-infected cells. Differently from activated CD4+ T lymphocytes, M/M are resistant to the cytopathic effect of HIV and survive HIV infection for a long time. Moreover, HIV-1 replication in M/M is a key pathogenetic event during the course of HIV-1 infection. Overall findings strongly support the clinical relevance of anti-HIV drugs in M/M. Nucleoside RT inhibitors (NRTIs) are more active against HIV in M/M than in CD4+ T lymphocytes. Their activity is further boosted by the presence of an additional monophosphate group (i.e., a phosphonate group, as in the case of Tenofovir), thus overcoming the bottleneck of the low phosphorylation ability of M/M. In contrast, the antiviral activity of non-NRTIs (not affecting the DNA chain elongation) in M/M is similar to that in CD4+ T lymphocytes. Protease inhibitors are the only clinically approved drugs acting at a late stage of the HIV lifecycle. They are able to interfere with HIV replication in HIV-1 chronically infected M/M, even if at concentrations greater than those observed in HIV-1 chronically infected CD4+ T lymphocytes. Finally, several new drugs have been shown to interfere efficiently with HIV replication in M/M, including entry inhibitors. A better understanding of the activity of the anti-HIV drugs in M/M may represent a key element for the design of effective anti-HIV chemotherapy.
Caveolin-1 Regulates Cellular Trafficking and Function of the Glucagon-Like Peptide 1 Receptor
Authors: Syme Colin A. , Zhang Lei , Bisello Alessandro 
Published: August 2006

AbstractThe glucagon-like peptide 1 receptor (GLP-1R) mediates important effects on β-cell function and glucose homeostasis and is one of the most promising therapeutic targets for type 2, and possibly type 1, diabetes. Yet, little is known regarding the molecular and cellular mechanisms that regulate its function. Therefore, we examined the cellular trafficking of the GLP-1R and the relation between receptor localization and signaling activity. In resting human embryonic kidney 293 and insulinoma MIN6 cells, a fully functional green fluorescent protein-tagged GLP-1R was localized both at the cell membrane and in highly mobile intracellular compartments. Real-time confocal fluorescence microscopy allowed direct visualization of constitutive cycling of the receptor. Overexpression of K44A-dynamin increased the number of functional receptors at the cell membrane. Immunoprecipitation, sucrose sedimentation, and microscopy observations demonstrated that the GLP-1R localizes in lipid rafts and interacts with caveolin-1. This interaction is necessary for membrane localization of the GLP-1R, because overexpression of a dominant-negative form of caveolin-1 (P132L-cav1) or specific mutations within the putative GLP-1R’s caveolin-1 binding domain completely inhibited GLP-1 binding and activity. Upon agonist stimulation, the GLP-1R underwent rapid and extensive endocytosis independently from arrestins but in association with caveolin-1. Finally, GLP-1R-stimulated activation of ERK1/2, which involves transactivation of epidermal growth factor receptors, required lipid raft integrity. In summary, the interaction of the GLP-1R with caveolin-1 regulates subcellular localization, trafficking, and signaling activity. This study provides further evidence of the key role of accessory proteins in specifying the cellular behavior of G protein-coupled receptors.
Synthesis of Hydrotalcite-Like Compounds and their Physico-Chemical Properties—The Systems Mg2+-Al3+-SO42− and Mg2+-Al3+-CrO42−
Authors: Miyata Shigeo , Okada Akira 
Published: August 2006

AbstractHydrotalcite-like compounds [MgI − xAlx(OH)2]x+ [A2−x/2, mH2O]x−, where A2− is SO42− or CrO42− and x ≇ 0.25, were prepared by a coprecipitation method and their physico-chemical properties were studied by X-ray diffraction, thermal analysis, i.r. absorption spectra and acidity-basicity measurement.The compounds including SO42− and CrO42− were analogous to an orthorhombic hydrotalcite and the lattice constants, a0 and c0 were 3.05 and 25.97 Å, and 3.05 and 26.48 Å, respectively. The crystallite size and strain in the 003 direction were 127 Å and 4.77 × 10−2 for the SO42− system or 83 Å and 6.60 × 10−2 for the CrO42− system, indicating a largely distorted microcrystallite. Two endothermic peaks observed at 240 and 455°C for the SO42− system and at 230 and 460°C for the CrO42− system are ascribed to the eliminations of interlayer water (mH2O) and structural water, respectively. The compound including SO42− formed MgO by calcination at 500°C and MgSO4 and MgAl2O4 by calcination at 800°C while the compound including CrO42− formed MgO at 400°C and MgAl2 − xCrxO4 at 800° I.r. study of the SO42− compound indicated that the bonding of SO42− was a bridge type. The highest acid and base strengths of the SO42− compound were H0 ≤ 1.5 and H− ≥ 12.2, the acidity and basicity being 0.3 and 0.1 mmol/g, respectively.