bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lacidophilin tablets alleviate constipation through regulation of intestinal microflora by promoting the colonization of Akkermansia sps
Authors: Sun Denglong , Yu Jingting , Zhan Yang , Cheng Xiaoying , Zhang Jingwen , Li Yingmeng , Li Qiong , Xiong Yanxia , Liu Wenjun 
Published: March 2024

AbstractConstipation is a major health problem worldwide that requires effective and safe treatment options. Increasing evidence indicates that disturbances in gut microbiota may be a risk factor for constipation. Administration of lacidophilin tablets shows promising therapeutic potential in the treatment of inflammatory bowel disease owing to their immunomodulatory properties and regulation of the gut microbiota. The focus of this study was on investigating the ability of lacidophilin tablets to relieve constipation by modulating the gut microbiome. Rats with loperamide hydrochloride induced constipation were treated with lacidophilin tablets via intragastric administration for ten days. The laxative effect of lacidophilin tablets was then evaluated by investigating the regulation of intestinal microflora and the possible underlying molecular mechanism. Our results reveal that treatment with lacidophilin tablets increased the intestinal advancement rate, fecal moisture content, and colonic AQP3 protein expression. It also improved colonic microflora structure in the colonic contents of model rats mainly by increasing Akkermansia muciniphila and decreasing Clostridium_sensu_stricto_1. Transcriptome analysis indicated that treatment with lacidophilin tablets maintains the immune response in the intestine and promotes recovery of the intestinal mechanical barrier in the constipation model. Our study shows that lacidophilin tablets improve constipation, possibly by promoting Akkermansia colonization and by modulating the intestinal immune response.
Homocysteine metabolites inhibit autophagy by upregulating miR-21-5p, miR-155-5p, miR-216-5p, and miR-320c-3p in human vascular endothelial cells
Authors: Witucki Łukasz , Jakubowski Hieronim 
Published: March 2024

AbstractNutritional and genetic deficiencies in homocysteine (Hcy) metabolism lead to hyperhomocysteinemia (HHcy) and cause endothelial dysfunction, a hallmark of atherosclerosis, which is a major cause of cardiovascular disease (CVD). Impaired autophagy causes the accumulation of damaged proteins and organelles and is associated with CVD. Biochemically, HHcy is characterized by elevated levels of Hcy and its metabolites, Hcy-thiolactone and N-Hcy-protein. However, whether these metabolites can dysregulate mTOR signaling and autophagy in endothelial cells is not known. Here, we examined the influence of Hcy-thiolactone, N-Hcy-protein, and Hcy on autophagy human umbilical vein endothelial cells. We found that treatments with Hcy-thiolactone, N-Hcy-protein, or Hcy significantly downregulated beclin 1 (BECN1), autophagy-related 5 (ATG5), autophagy-related 7 (ATG7), and microtubule-associated protein 1 light chain 3 (LC3) mRNA and protein levels. We also found that these changes were mediated by upregulation by Hcy-thiolactone, N-Hcy-protein, and Hcy of autophagy-targeting microRNA (miR): miR-21, miR-155, miR-216, and miR-320c. The effects of these metabolites on levels of miR targeting autophagy as well as on the levels of BECN1, ATG5, ATG7, and LC3 mRNA and protein were abrogated by treatments with inhibitors of miR-21, miR-155, miR-216, and mir320c. Taken together, our findings show that Hcy metabolites can upregulate miR-21, miR-155, miR-216, and mir320c, which then downregulate autophagy in human endothelial cells, important for vascular homeostasis.
A theoretical model for roller-shape design of three-roller continuous and synchronous calibration process of ovality and straightness for large thin-walled pipes
Authors: Huang Xueying , Gu JinPing 
Published: March 2024

AbstractLarge thin-walled pipes are particularly suitable for oil and gas transport and auxiliary pipes in cold areas or deep sea-beds. At present, the rounding and straightening processes are completed independently, and the theoretical model for roller-shape design is analyzed only in a single direction. To solve this problem, a theoretical model for roller-shape design of three-roller continuous and synchronous adjusting straightness and ovality process for large thin-walled pipes is established. The established model is verified by numerical simulation and experimental research using 304 stainless steel pipes. The results show that the three roller-shape design schemes, including three-section, four-section and five-section, proposed based on the theoretical model, can obtain qualified formed pipes. Based on the model, the residual ovality, residual straightness and maximum residual stress of the three roller-shape schemes are discussed. The residual straightness can reach within 0.2%, and the residual ovality can reach within 1%. It verifies the applicability of the model and the feasibility of the process. The model can provide a theoretical basis for presetting the process parameters and optimizing the roller-shape.
Green tea and hyaluronic acid gel enhance fibroblast activation and improves the gingival healing post-third molar extraction
Authors: Bonatto Mariana da Silva , Feltran Geórgia da Silva , Barbosa Thamires Prazeres , Pereira Davisson Alves , Santos Samara de Souza , Mendes Pedro Gomes Junqueira , e Pessoa Roberto Sales , Bezerra Fábio José Barbosa , Zambuzzi Willian Fernando , de Oliveira Guilherme José Pimentel Lopes 
Published: March 2024

AbstractThis study evaluates the effects of a green tea (Camellia sinensis) and hyaluronic acid gel on fibroblast activity and alveolar bone repair following third molar extractions. By examining the gene expression related to cell survival, proliferation, and angiogenesis, the study bridges in vitro findings with clinical outcomes in a split-mouth randomized trial. Human fibroblasts were exposed to the treatment gel, analysing gene expression through RT-qPCR. Twenty participants undergoing bilateral third molar extractions received the test gel on one side and a placebo on the other. Assessments included patient-reported outcomes, professional evaluations, and radiographic analyses at multiple postoperative intervals. The test gel significantly enhanced AKT, CDKs, and VEGF gene expressions, indicating a positive effect on angiogenesis and cell proliferation. Clinically, it resulted in reduced exudate, swelling, and secondary interventions, with radiographs showing improved alveolar bone density after 90 days. The green tea and hyaluronic acid gel significantly improves soft tissue and bone healing post-extraction, offering a promising adjunctive therapy for enhancing postoperative recovery. This gel represents a novel adjuvant treatment option for facilitating improved healing outcomes after third molar extractions, highlighting its potential utility in clinical dental practice.
PDGFRA K385 mutants in myxoid glioneuronal tumors promote receptor dimerization and oncogenic signaling
Authors: de Villenfagne Laurence , Sablon Ariane , Demoulin Jean-Baptiste 
Published: March 2024

AbstractMyxoid glioneuronal tumors (MGNT) are low-grade glioneuronal neoplasms composed of oligodendrocyte-like cells in a mucin-rich stroma. These tumors feature a unique dinucleotide change at codon 385 in the platelet-derived growth factor receptor α (encoded by the PDGFRA gene), resulting in the substitution of lysine 385 into leucine or isoleucine. The functional consequences of these mutations remain largely unexplored. Here, we demonstrated their oncogenic potential in fibroblast and Ba/F3 transformation assays. We showed that the K385I and K385L mutants activate STAT and AKT signaling in the absence of ligand. Co-immunoprecipitations and BRET experiments suggested that the mutations stabilized the active dimeric conformation of the receptor, pointing to a new mechanism of oncogenic PDGF receptor activation. Furthermore, we evaluated the sensitivity of these mutants to three FDA-approved tyrosine kinase inhibitors: imatinib, dasatinib, and avapritinib, which effectively suppressed the constitutive activity of the mutant receptors. Finally, K385 substitution into another hydrophobic amino acid also activated the receptor. Interestingly, K385M was reported in a few cases of brain tumors but not in MGNT. Our results provide valuable insights into the molecular mechanism underlying the activation of PDGFRα by the K385I/L mutations, highlighting their potential as actionable targets in the treatment of myxoid glioneuronal tumors.
Camptothecin enhances the anti-tumor effect of low-dose apatinib combined with PD-1 inhibitor on hepatocellular carcinoma
Authors: Wang Hankang , Gao Congcong , Li Xiaodong , Chen Feng , Li Guijie 
Published: March 2024

AbstractApatinib has been shown to apply to a variety of solid tumors, including advanced hepatocellular carcinoma. Preclinical and preliminary clinical results confirmed the synergistic antitumor effects of apatinib in combination with anti-programmed death-1 (PD-1) inhibitors. In this study, we investigated camptothecin (CPT) enhances the anti-tumor effect of low-dose apatinib combined with PD-1 inhibitor on hepatocellular carcinoma. CPT combined with a PD-1 inhibitor enhances the anti-tumor effects of low-dose apatinib in hepatocellular carcinoma which was evaluated in making use of the H22 mouse model (n = 32), which was divided into four groups. Immunohistochemical staining and western blotting were used to detect nuclear factor erythroid 2-related factor 2 (Nrf2) as well as sequestosome 1 (p62), vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), PD-1, and programmed cell death ligand 1 (PD-L1). The results showed that the average size of the tumor of the combination group (Group D) was significantly less than that of the apatinib + PD-1 inhibitor group (Group C). The expression levels of Nrf2, p62, VEGFA, VEGFR2, PD-1, and PD-L1 in the apatinib + PD-1 inhibitor group(Group C) were lower than those in the control group (Group A) (P < 0.05). The expression levels of these genes in the apatinib + PD-1 inhibitor group (Group C) were significantly lower in the combination group (Group D) (P < 0.05). There was no obvious difference in body weight and liver and kidney functions between the four groups of mice. In conclusion, CPT improves the anti-tumor effect of low-dose apatinib combined with PD-1 inhibitor on hepatocellular carcinoma
Assessment of hypercholesterolemia prevalence and its demographic variations in the Republic of Kazakhstan
Authors: Kulimbet Mukhtar , Davletov Kairat , Saliev Timur , Davletov Dimash , Dzhumabekov Berik , Umbayev Bauyrzhan , Balabayeva Aigerim , Tanabayeva Shynar , Fakhradiyev Ildar 
Published: March 2024

AbstractHypercholesterolemia is a major risk factor for cardiovascular disease, the leading cause of death in Kazakhstan. Understanding its prevalence is vital for effective public health planning and interventions. This study aimed to assess the scale of hypercholesterolemia in the Republic of Kazakhstan and to identify differences among distinct population groups. A cross-sectional study involving 6720 participants (a nationally representative survey.) aged 18–69 was conducted from October 2021 to May 2022 across all 17 regions of Kazakhstan. The magnitude of hypercholesterolemia was 43.5%. Cholesterol levels were determined through blood biochemical analysis. Age, sex, geographic location, and ethnicity served as covariates. The majority of participants (65.49%) were from urban areas with an almost equal gender distribution (50.07% male and 49.93% female). The predominant age groups were 18–29 years (25.71%) and 30–39 years (25.12%), and 65.09% identified as Kazakh. The prevalence increased with age, with the 60–69 age group showing the highest rate at 71.14%. Women had slightly higher rates than men. Geographical differences were evident, with regions like Astana city and Almaty region showing significant disparities. Kazakhs had a lower rate compared to other ethnicities. Age, region, and BMI were significant predictors for hypercholesterolemia in both binary and multivariate logistic regression analyses. The study revealed a significant prevalence of hypercholesterolemia in Kazakhstan, with increasing age as a major determinant. Women, especially those over 50, and certain regions showed higher cholesterol levels. The disparities observed across regions and ethnicities suggest the need for targeted public health interventions to address this pressing health concern.
The C-type lectin DCIR contributes to the immune response and pathogenesis of colorectal cancer
Authors: Trimaglio Giulia , Sneperger Tamara , Raymond Benjamin B. A. , Gilles Nelly , Näser Emmanuelle , Locard-Paulet Marie , Ijsselsteijn Marieke E. , Brouwer Thomas P. , Ecalard Romain , Roelands Jessica , Matsumoto Naoki , Colom André , Habch Myriam , de Miranda Noel F. C. C. , Vergnolle Nathalie , Devaud Christel , Neyrolles Olivier , Rombouts Yoann 
Published: March 2024

AbstractDevelopment and progression of malignancies are accompanied and influenced by alterations in the surrounding immune microenvironment. Understanding the cellular and molecular interactions between immune cells and cancer cells has not only provided important fundamental insights into the disease, but has also led to the development of new immunotherapies. The C-type lectin Dendritic Cell ImmunoReceptor (DCIR) is primarily expressed by myeloid cells and is an important regulator of immune homeostasis, as demonstrated in various autoimmune, infectious and inflammatory contexts. Yet, the impact of DCIR on cancer development remains largely unknown. Analysis of available transcriptomic data of colorectal cancer (CRC) patients revealed that high DCIR gene expression is associated with improved patients’ survival, immunologically "hot" tumors and high immunologic constant of rejection, thus arguing for a protective and immunoregulatory role of DCIR in CRC. In line with these correlative data, we found that deficiency of DCIR1, the murine homologue of human DCIR, leads to the development of significantly larger tumors in an orthotopic murine model of CRC. This phenotype is accompanied by an altered phenotype of tumor-associated macrophages (TAMs) and a reduction in the percentage of activated effector CD4+ and CD8+ T cells in CRC tumors of DCIR1-deficient mice. Overall, our results show that DCIR promotes antitumor immunity in CRC, making it an attractive target for the future development of immunotherapies to fight the second deadliest cancer in the world.
Electrochemical properties of MnSe inclusions and improving the pitting corrosion resistance of stainless steel via Se microalloying
Authors: Nishimoto Masashi , Katsuyama Tomoki , Muto Izumi 
Published: March 2024

AbstractControlling the electrochemical properties of non-metallic inclusions is of substantial interest in the design of corrosion-resistant alloys. To the best of our knowledge, the dissolution and pitting corrosion behavior of selenide inclusions in stainless steels and the improvement of the pitting corrosion resistance of type 304 stainless steels via Se microalloying have not been investigated. In this study, stainless steel specimens containing artificial MnS and MnSe inclusions were fabricated via spark plasma sintering to systematically investigate their electrochemical properties. The superior pitting corrosion resistance and dissolution resistance of MnSe to those of MnS were demonstrated. The results obtained from the sintered specimens were applied to improve the pitting corrosion resistance of type 304 stainless steels via Se microalloying. Adding a trace amount of Se (0.005 mass%) altered the readily soluble sulfide inclusions to dissolution-resistant selenide-type inclusions, resulting in improved pitting corrosion resistance of type 304 stainless steel.
Analysis of endoscopic and pathological features of 6961 cases of gastric cancer
Authors: Lu Junhui , Wang Qing , Zhang Hezhao , Liu Jingwei , Ren Jinnan , Fan Jing , Gong Jingwen , Sui Yue , Chen Xing 
Published: March 2024

AbstractGastric cancer (GC) stage and tissue differentiation affect treatment efficacy and prognosis, highlighting the importance of understanding the risk factors that affect these parameters. Therefore, this study analyzed risk factors affecting the GC stage and differentiation and the relationships between the cancer site and the sex and age of the patient. We collected clinical data from 6961 patients with GC, including sex, age, endoscopic lesion location, and pathological differentiation. Patients were grouped based on GC stage (early or advanced), differentiation (well or poorly differentiated), and lesion site (upper stomach [cardia and fundus], middle stomach [gastric body], and lower stomach [gastric antrum]). Differences in sex, age, location, stage, and degree of differentiation were assessed based on these groupings. Univariate analysis revealed that the disease location and differentiation significantly differed based on the GC stage (P < 0.05), whereas sex, age, site, and stage significantly differed based on GC differentiation (P < 0.05). A multivariate analysis confirmed these factors as independent risk factors affecting GC. Moreover, lesion sites significantly differed between sexes (P < 0.05) and among age groups (P < 0.05). Although the effects of family history, lifestyle, and Helicobacter pylori infection status of the patients were not considered, this single-center retrospective study established independent risk factors for GC.Trial registration ChiCTR2200061989.
Research on collaborative edge network service migration strategy based on crowd clustering
Authors: Cao Junjie , Yu Zhiyong , Xue Bin 
Published: March 2024

AbstractThe innovative application of Crowd Intelligent Devices (CIDS) in edge networks has garnered attention due to the rapid development of artificial intelligence and computer technology. This application offers users more reliable and low-latency computing services through computation offloading technology. However, the dynamic nature of network terminals and the limited coverage of edge servers pose challenges, such as data loss and service interruption. Furthermore, the high-speed mobility of intelligent terminals in the dynamic edge network environment further complicates the design of computation offloading and service migration strategies. To address these challenges, this paper explores the computation offloading model of cluster intelligence collaboration in a heterogeneous network environment. This model involves multiple intelligences collaborating to provide computation offloading services for terminals. To accommodate various roles, a switching strategy of split-cluster group collaboration is introduced, assigning the cluster head, the alternate cluster head, and the ordinary user are assigned to a group with different functions. Additionally, the paper formulates the optimal offloading strategy for group smart terminals as a Markov decision process, taking into account factors such as user mobility, service delay, service accuracy, and migration cost. To implement this strategy, the paper utilizes the deep reinforcement learning-based CCSMS algorithm. Simulation results demonstrate that the proposed edge network service migration strategy, rooted in groupwise cluster collaboration, effectively mitigates interruption delay and enhances service migration efficiency.
Nature-based Solutions can help restore degraded grasslands and increase carbon sequestration in the Tibetan Plateau
Authors: Sun Jian , Wang Yingxin , Lee Tien Ming , Nie Xiaowei , Wang Tao , Liang Eryuan , Wang Yafeng , Zhang Lin , Wang Jun , Piao Shilong , Chen Fahu , Fu Bojie 
Published: March 2024

AbstractThe Tibetan grassland ecosystems possess significant carbon sink potential and have room for improved carbon sequestration processes. There is a need to uncover more ambitious and coherent solutions (e.g., Nature-based Solutions) to increase carbon sequestration. Here, we investigated the rationale and urgency behind the implementation of Nature-based Solutions on sequestering carbon using literature review and meta-analysis. We also project the changes in terrestrial carbon sink of Tibetan Plateau grassland ecosystems using model simulations with different future emissions scenario. The results show that the Nature-based Solution projects are expected to increase the carbon sink of Tibetan Plateau grassland ecosystems by 15 to 21 tetragrams of carbon by 2060. We defined a conceptual framework of Nature-based Solutions that integrates initiatives for the restoration of degraded grasslands and carbon sequestration. Our framework consists of four stages: theory, identification, practice, and goal. Traditional Tibetan knowledge plays an important role in reframing the proposed Nature-based Solutions framework. We also apply this framework to optimize ecological restoration techniques and projects and to evaluate the annual changes in the carbon sink under different socioeconomic pathway scenarios.
Microbial conversion of carbon dioxide into premium medium-chain fatty acids: the progress, challenges, and prospects
Authors: Wu Kai-Kai , Xu Pian-Pian , Zhao Lei , Ren Nan-Qi , Zhang Yi-Feng 
Published: March 2024

AbstractThe conversion of carbon dioxide to medium-chain fatty acids (CO2-to-MCFAs) through microbial processes represents a valuable technology for sequestering and exploiting CO2, generating superior bio-chemicals from the primary contributor to the greenhouse effect. However, a comprehensive overview and generalization of microbial CO2-to-MCFAs are presently deficient. Based on this, the present review systematically summarizes the research progress, explicates the process mechanisms, analyses the key challenges and possible solutions, and anticipates forthcoming research perspectives and priorities for the first time. We proposed two original strategies, namely the synchronous strategy and integrated strategy, from current research into microbial CO2-to-MCFAs. The synchronous strategy concurrently achieves hydrogen (H2) and CO2 assimilation, as well as MCFAs production, by employing a reactor that co-cultivates predominant H2/CO2-utilizing microorganisms and chain elongation microorganisms. The integrated approaches involve CO2-to-precursors (i.e., acetate and ethanol) and subsequent precursors-to-MCFAs, achieved through the use of two bioreactors for separately cultivating H2/CO2-utilizing microorganisms and chain elongation microorganisms. Mechanistic insights reveal that microbial CO2-to-MCFAs predominantly encompasses two processes: H2 and CO2 assimilation into precursor and subsequent precursors chain elongation into MCFAs, through a Wood-Ljungdahl pathway and a two-round elongation, respectively. The analyses of key challenges and possible solutions for microbial CO2-to-MCFAs underscore the imperative to enhance efficiency and economy and to shed light on metabolic mechanisms. Furthermore, in order to improve the strategy application potential of microbial CO2-to-MCFAs, future research perspectives and priorities, e.g. exploitation of functional pure bacteria, screening of functional pure bacteria, multi-omics analysis, genetic modification and enhancement, enhancement of bioreactor stability, specific MCFA production, development of coupled purification technology for MCFAs, and economic benefits and ecological environmental risks, are proposed and prospected. This work is expected to offer a thorough understanding of the microbial CO2-to-MCFAs, guide and inspire researchers to address critical challenges in-depth and propel the development of CO2-to-MCFAs.