bluuebunny/crossref_metadata_2025_split huggingface dataset:
The antifungal activity of the terrestrial Streptomyces US80 strain is induced by heat‐killed fungi
Authors: Fourati‐Ben Fguira Lilia , Smaoui Slim , Karray‐Rebai Ines , Bejar Samir , Mellouli Lotfi 
Published: May 2008

AbstractStudy of the influence of different concentrations of glucose, as carbon source, and magnesium, as chemical additive, on production by the Streptomyces sp. US80 strain of the three antifungal molecules (irumamycin, X‐14952 B and 17‐hydroxy‐venturicidin A) showed that the highest antifungal activity was obtained at 5 g/L and 3.5 mM for glucose and magnesium, respectively. Environmental factors for maximum antifungal activity production are: temperature of growth 30°C, pH 7, incubation time 72 h and agitation rate of 200 rpm. To further enhance the production of the three antifungal compounds, which possess a real potential application in the agriculture domain, and to explore the possibility of obtaining other active molecules from the Streptomyces sp. US80, we investigated the effect of the addition of heat‐killed fungi to the culture media. Biochemical, microbiological and spectroscopic studies of the cultures of the Streptomyces sp. US80 strain in absence (control) and in presence of heat‐killed fungus cells indicate an increase of 70% in the production of the three antifungal molecules, compared to the control culture.
Prevention of radial artery occlusion—Patent hemostasis evaluation trial (PROPHET study): A randomized comparison of traditional versus patency documented hemostasis after transradial catheterization
Authors: Pancholy Samir , Coppola John , Patel Tejas , Roke‐Thomas Marie 
Published: May 2008

AbstractObjective: The objective of this study was to evaluate the efficacy of hemostasis with patency in avoiding radial artery occlusion after transradial catheterization. Background: Radial artery occlusion is an infrequent but discouraging complication of transradial access. It is related to factors such as sheath to artery ratio and is less common in patients receiving heparin. Despite being clinically silent in most cases, it limits future transradial access. Patients and Methods: Four hundred thirty‐six consecutive patients undergoing transradial catheterization were prospectively enrolled in the study. Two hundred nineteen patients were randomized to group I, and underwent conventional pressure application for hemostasis. Two hundred seventeen patients were randomized to group II and underwent pressure application confirming radial artery patency using Barbeau's test. Radial artery patency was studied at 24 hr and 30 days using Barbeau's test. Results: Thirty‐eight patients had evidence of radial artery occlusion at 24 hr. Twenty patients had persistent evidence of radial artery occlusion at 1 month. Group II, with documented patency during hemostatic compression, had a statistically and clinically lower incidence of radial artery occlusion (59% decrease at 24 hr and 75% decrease at 30 days, P < 0.05), compared with patients in group I. Low body weight patients were at significantly higher risk of radial artery occlusion. No procedural variables were found to be associated with radial artery occlusion. Conclusion: Patent hemostasis is highly effective in reducing radial artery occlusion after radial access and guided compression should be performed to maintain radial artery patency at the time of hemostasis, to prevent future radial artery occlusion. © 2008 Wiley‐Liss, Inc.
Reversible Transitions between Peptide Nanotubes and Vesicle‐Like Structures Including Theoretical Modeling Studies
Authors: Yan Xuehai , Cui Yue , He Qiang , Wang Kewei , Li Junbai , Mu Weihua , Wang Bolin , Ou‐yang Zhong‐can 
Published: May 2008

AbstractPeptide‐based self‐assembling systems are increasingly attractive because of their wide range of applications in different fields. Peptide nanostructures are flexible with changes in the ambient conditions. Herein, a reversible shape transition between self‐assembled dipeptide nanotubes (DPNTs) and vesicle‐like structures is observed upon a change in the peptide concentration. SEM, TEM, AFM, and CD spectroscopy were used to follow this transition process. We show that dilution of a peptide‐nanotube dispersion solution results in the formation of vesicle‐like structures, which can then be reassembled into the nanotubes by concentrating the solution. A theoretical model describing this shape‐transition phenomenon is presented to propose ways to engineer assembling molecules in order to devise other systems in which the morphology can be tuned on demand.
Live‐cell analysis of mitotic spindle formation in taxol‐treated cells
Authors: Hornick Jessica E. , Bader Jason R. , Tribble Emily K. , Trimble Kayleigh , Breunig J. Scott , Halpin Elizabeth S. , Vaughan Kevin T. , Hinchcliffe Edward H. 
Published: May 2008

AbstractTaxol functions to suppress the dynamic behavior of individual microtubules, and induces multipolar mitotic spindles. However, little is known about the mechanisms by which taxol disrupts normal bipolar spindle assembly in vivo. Using live imaging of GFP‐α tubulin expressing cells, we examined spindle assembly after taxol treatment. We find that as taxol‐treated cells enter mitosis, there is a dramatic re‐distribution of the microtubule network from the centrosomes to the cell cortex. As they align there, the cortical microtubules recruit NuMA to their embedded ends, followed by the kinesin motor HSET. These cortical microtubules then bud off to form cytasters, which fuse into multipolar spindles. Cytoplasmic dynein and dynactin do not re‐localize to cortical microtubules, and disruption of dynein/dynactin interactions by over‐expression of p50 “dynamitin” does not prevent cytaster formation. Taxol added well before spindle poles begin to form induces multipolarity, but taxol added after nascent spindle poles are visible—but before NEB is complete—results in bipolar spindles. Our results suggest that taxol prevents rapid transport of key components, such as NuMA, to the nascent spindle poles. The net result is loss of mitotic spindle pole cohesion, microtubule re‐distribution, and cytaster formation. Cell Motil. Cytoskeleton 2008. © 2008 Wiley‐Liss, Inc.
Developmental and comparative aspects of posterior medial thalamocortical innervation of the barrel cortex in mice and rats
Authors: Kichula Elizabeth A. , Huntley George W. 
Published: May 2008

AbstractThe thalamocortical projection to the rodent barrel cortex consists of inputs from the ventral posterior medial (VPM) and posterior medial (POm) nuclei that terminate in largely non‐overlapping territories in and outside of layer IV. This projection in both rats and mice has been used extensively to study development and plasticity of highly organized synaptic circuits. Whereas the VPM pathway has been well characterized in both rats and mice, organization of the POm pathway has only been described in rats, and no studies have focused exclusively on the development of the POm projection. Here, using transport of Phaseolus vulgaris leucoagglutinin (PHA‐L) or carbocyanine dyes, we characterize the POm thalamocortical innervation of adult mouse barrel cortex and describe its early postnatal development in both mice and rats. In adult mice, POm inputs form a dense plexus in layer Va that extends uniformly underneath layer IV barrels and septa. Innervation of layer IV is very sparse; a clear septal innervation pattern is evident only at the layer IV/Va border. This pattern differs subtly from that described previously in rats. Developmentally, in both species, POm axons are present in barrel cortex at birth. In mice, they occupy layer IV as it differentiates, whereas in rats, POm axons do not enter layer IV until 1–2 days after its emergence from the cortical plate. In both species, arbors undergo progressive and directed growth. However, no layer IV septal innervation pattern emerges until several days after the cytoarchitectonic appearance of barrels and well after the emergence of whisker‐related clusters of VPM thalamocortical axons. The mature pattern resolves earlier in rats than in mice. Taken together, these data reveal anatomical differences between mice and rats in the development and organization of POm inputs to barrel cortex, with implications for species differences in the nature and plasticity of lemniscal and paralemniscal information processing. J. Comp. Neurol. 509:239–258, 2008. © 2008 Wiley‐Liss, Inc.
CD4+ T‐lymphocyte enumeration with a flow‐rate based method in three flow cytometers with different years in service
Authors: Pattanapanyasat Kovit , Chimma Pattamawan , Sratongno Panudda , Lerdwana Surada 
Published: May 2008

AbstractBackgroundCD4+ T‐lymphocyte count remains the most important surrogate marker for management of HIV patients in resource‐poor countries. The standard single‐platform (SP) bead‐based flow cytometric method for CD4+ testing is expensive; more affordable methods are needed. We evaluated the SP flow‐rate‐based calibration method for determining CD4+ counts, using three flow cytometers of varying ages.MethodsCD4+ counts from 103 HIV‐1 infected Thai patients were determined using a SP flow‐rate method in flow cytometers with 2, 12, and 16 years of service. Results were compared to the bead‐based method. Correlation and agreement were analyzed using linear regression and Bland‐Altman analysis.ResultsCounts obtained from the flow‐rate approach in each flow cytometer showed strong correlation with the bead‐based method (R2 = 0.97, 0.97, and 0.96 for the 2‐, 12‐, and 16‐year‐old flow cytometers). The mean biases for the flow‐rate approach compared with the bead‐based method were +32.4 cells/μL (limit of agreement (LOA): −83.0 to +147.8 cells/μL), −28.8 cells/μL (LOA: −131.6 to +74.1 cells/μL), and −27.0 cells/μL (LOA: −149.4 to +95.4 cells/μL).ConclusionThe flow‐rate approach is reliable for determining CD4+ counts. Results do not vary by age of flow cytometer. This approach provides a cost‐effective alternative for HIV patient monitoring in resource‐poor settings. © 2008 Clinical Cytometry Society
Libraries of Bidentate Phosphorus Ligands; Synthesis Strategies and Application in Catalysis
Authors: Goudriaan P. Elsbeth , van Leeuwen Piet W. N. M. , Birkholz Mandy‐Nicole , Reek Joost N. H. 
Published: May 2008

AbstractCombinatorial chemistry in combination with high‐throughput screening technologies is an important way of finding new successful catalyst systems. The design of ligand libraries of bidentate phosphorus ligands and the application of their transition‐metal complexes in homogeneous (asymmetric) catalysis reactions will be described in this review. Till now three different approaches were developed to arrive at such libraries of bidentate phosphorus ligands: 1) modular synthesis of bidentate ligands 2) the solid support synthesis of bidentate ligands and 3) the self‐assembly of ligand building blocks into bidentate ligands. The scope and limitations of these strategies will be discussed on the basis of a limited number of articles that dealt with the synthesis of at least 15 bidentate phosphorus ligands. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Quaterpyridine Ligands Forming Helical Complexes of Mono‐ and Dinuclear (Helicate) Forms
Authors: Stefankiewicz Artur R. , Wałęsa Monika , Jankowski Piotr , Ciesielski Artur , Patroniak Violetta , Kubicki Maciej , Hnatejko Zbigniew , Harrowfield Jack M. , Lehn Jean‐Marie 
Published: May 2008

AbstractThe dimethylquaterpyridine ligand L1 reacts with CuI and AgI to give dinuclear complexes of “helicate” form, in which the metals are in an approximate tetrahedral coordination, whereas reactions with CoII and ZnII provide mononuclear complexes, in which the metals are apparently six‐coordinate with markedly distorted octahedral coordination, and the ligand retains a helical conformation. Oxidation of the methyl substituents of L1 into carboxylic acid groups, followed by esterification, gives ligand L2, which also forms a dinuclear CuI helicate and a mononuclear CoII complex; one of the ester groups appears to be involved in complexation in the latter. All the complexes were characterised by single‐crystal X‐ray structure determinations.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Synthesis and Characterization of Acetonitrile‐Ligated Transition‐Metal Complexes with Tetrakis(pentafluorophenyl)borate as Counteranions
Authors: Hijazi Ahmed K. , Al Hmaideen Akef , Syukri Syukri , Radhakrishnan Narayanan , Herdtweck Eberhardt , Voit Brigitte , Kühn Fritz E. 
Published: May 2008

AbstractComplexes with the general formula [MII(NCCH3)6][TPFB]2 [M = Cr, Fe, Co, Ni, Cu, Zn, (TPFB)– = tetrakis(pentafluorophenyl)borate] were synthesized and characterized both in the solid state and in solution. According to the spectroscopic data, [TPFB] can be considered as a truly noncoordinating anion. The NCCH3 ligands are lost if the samples are kept at room temperature for extended periods of time. Thermolysis leads to the loss of the NCCH3 ligands and decomposition of the anion above 100 °C with the formation of MF2. It has to be noted that distortion of the geometries of the CuII, ZnII and CrII complexes occurs, as evidenced by infrared spectroscopy. The complexes can be easily prepared and obtained in high yields and are moderately sensitive to air. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Redox‐Controlled Fluorescence Modulation in a BODIPY‐Quinone Dyad (Eur. J. Org. Chem. 16/2008)
Authors: Benniston Andrew C. , Copley Graeme , Elliott Kristopher J. , Harrington Ross W. , Clegg William 
Published: May 2008

AbstractThe cover picture shows the chemically induced reversible cycling of a BODIPY‐based dyad from the dark quinone state to the highly emissive hydroquinone form. The change in luminescence intensity can be readily seen by the eye. The phenylene spacer between the BODIPY and the redox‐active units is essential to the operation of the molecular dyads. Without this group, the fluorescence from the BODIPY group is quenched in both the quinone and hydroquinone forms. The reason for the difference in behaviour is discussed in the article by A. C. Benniston et al. on p. 2705 ff, along with the full details of the synthesis, structural, and sensing capabilities of the molecular systems. The authors thank the EPSRC for financial support of the work.
Association between mitochondrial DNA copy number, blood cell counts, and occupational benzene exposure
Authors: Shen Min , Zhang Luoping , Bonner Matthew R. , Liu Chin‐San , Li Guilan , Vermeulen Roel , Dosemeci Mustafa , Yin Songnian , Lan Qing 
Published: May 2008

AbstractBenzene is a recognized hematotoxicant and carcinogen that produces genotoxic damage. Benzene metabolites can produce reactive oxidative species. Mitochondrial DNA (mtDNA) copy number may be increased in response to oxidative stress to compensate for damaged mitochondria. We carried out a cross‐sectional study of 40 benzene‐exposed workers and 40 controls to evaluate the association between benzene exposure and mtDNA copy number. Copy number of mtDNA in leukocyte DNA was determined by real‐time PCR. Compared with controls, the copy number of mtDNA increased by 4% and by 15% in workers exposed to ≤10 ppm (n = 20) and >10 ppm (n = 20) benzene, respectively. After adjusting for recent infection, the factor that was significantly correlated with mtDNA, the increase of mtDNA was statistically significant in the high exposed group (P = 0.016) with a significant linear trend (P = 0.024). To our best knowledge, this is the first report that benzene exposure was associated with increased mitochondria DNA copy number. Benzene exposure may induce mtDNA amplification, possibly in response to oxidative stress caused by benzene. The finding needs to be replicated by other studies. Environ. Mol. Mutagen., 2008. © 2008 Wiley‐Liss, Inc.
Theoretical and experimental analysis of debris flow: rheology and two‐phase modelling
Authors: Mambretti Stefano , Larcan Enrico , De Wrachien Daniele 
Published: May 2008

AbstractTo predict debris flow dynamics a numerical model, based on 1D De Saint Venant (SV) equations, was developed. The McCormack–Jameson shock capturing scheme was employed for the solution of the equations, written in a conservative law form. This technique was applied to determine both the propagation and the profile of a two‐phase debris flow resulting from the instantaneous and complete collapse of a storage dam.To validate the model, comparisons have been made between its predictions and laboratory measurements concerning flows of water and homogeneous granular mixtures in a uniform geometry flume reproducing dam‐break waves. Agreement between computational and experimental results is considered very satisfactory for mature (non‐stratified) debris flows, which embrace most real cases. To better predict immature (stratified) flows, the model should be improved in order to feature, in a more realistic way, the distribution of the particles of different size within the mixture.On the whole, the proposed model can easily be extended to channels with arbitrary cross sections for debris flow routing, as well as for solving different problems of unsteady flow in open channels by incorporating the appropriate initial and boundary conditions. Copyright © 2008 John Wiley & Sons, Ltd.
A role for neuroglobin: Resetting the trigger level for apoptosis in neuronal and retinal cells
Authors: Fago Angela , Mathews Antony J. , Brittain Thomas 
Published: May 2008

AbstractWe propose a new hypothesis for the molecular mechanism by which neuroglobin exerts its protective effect in hypoxia‐induced cell death. Our recent observation of a very rapid electron‐transfer reaction between ferrous neuroglobin and ferric cytochrome c is central to this hypothesis. In contrast to previously suggested roles for neuroglobin, related to its putative but unlikely oxygen storage/transport properties or its ability to react with nitrogen oxides, we suggest that ferrous neuroglobin exerts its protective effect via modulation of the early events in the intrinsic apoptotic pathway. We suggest this is achieved by the rapid reduction of cytosolic ferric cytochrome c by neuroglobin. The maintenance of cytochrome c in the nonapoptotic ferrous oxidation state and the concomitant generation of ferric neuroglobin in this reaction fit well with known feedback processes in the early events of the intrinsic apoptotic pathway. Our hypothesis also fits well with a number of previously uncorrelated findings, including the localization of neuroglobin in close proximity to mitochondria, the high concentration of neuroglobin in cells whose basal rates of aerobic metabolism are extremely high, and the cell types which are subject to large calcium ion fluxes in their normal physiology. © 2008 IUBMB IUBMB Life, 60(6): 398–401, 2008
The mitochondrial gateway to cell death
Authors: Smith Danielle J. , Ng Heling , Kluck Ruth M. , Nagley Phillip 
Published: May 2008

AbstractMitochondria play a key role in death signaling. The intermembrane space of these organelles contains a number of proteins which promote cell death once they are redistributed to the cytosol. The formation of pores in the outer membrane of mitochondria defines a gateway through which the apoptogenic proteins pass during death signaling. Interactions between pro‐apoptotic and pro‐survival members of the Bcl‐2 family of proteins are decisive in the initiation of pore opening. While the specific composition of the pore in molecular terms is still subject to debate and continuing investigation, it is recognized functionally as a passive channel which not only allows egress of proteins to cytosol but also entry in the reverse direction. A variety of constraints may restrict the release of proteins from the intermembrane space to the cytosol. These include trapping in the intercristal spaces formed by the convoluted invaginations of the inner membrane, binding of proteins to the inner membrane or to other soluble proteins of the intermembrane space, or insertion of proteins into the inner membrane. There is a corresponding variety of mechanisms that facilitate release of apoptogenic proteins from such entrapment. Morphological changes that expand the inner membrane enable proteins to be released from enclosure in intercristal spaces, allowing these proteins access to the mitochondrial gateway. Specific cases include cytochrome c molecules bound to inner membrane cardiolipin and released upon oxidation of that lipid component. Further, AIF that is embedded in the inner membrane is released by proteases (caspases or calpains), which enter from the cytosol once the outer membrane pore has opened. The facilitation (or restriction) of apoptogenic protein release through the mitochondrial gateway may provide new opportunities for regulating cell death. © 2008 IUBMB IUBMB Life, 60(6): 383–389, 2008.
Control of Polyaniline Particle Shapes
Authors: Jung Woo‐Hyuk , Lee Young Moo , Jo Nam‐Ju , Lee Jang‐Oo 
Published: May 2008

AbstractNeedle‐like poly(aniline p‐toluenesulfonic acid) complex aggregates were synthesized by oxidative polymerization, yielding conductivities of up to 3 S · cm−1 and maximum aspect ratios of 26. Rod‐like aggregates as precursors to be developed into needle‐like aggregates were obtained with 1 to 2 h APS addition time at 15 °C. Flake‐like polyanilines were prepared at −2 or +20 °C with 1.9 to 2.5 h of APS addition time, giving aspect ratios of 2 to 10. Plate‐like polyaniline aggregates emerged when xylene was added, and an optimum xylene concentration was 15 vol.‐%, giving 1.3 S · cm−1 conductivity. The polyanilines were characterized by optical microscopy, SEM and UV‐vis/NIR spectroscopy.magnified image
Structure elucidation and complete NMR spectral assignments of two new dammarane‐type tetraglycosides from Panax quinquefolium
Authors: Jiang Hai‐Ping , Qiu Ying‐Kun , Cheng Da‐Ren , Kang Ting‐Guo , Dou De‐Qiang 
Published: May 2008

AbstractTwo new saponins were isolated from leaves of Panax quinquefolium and their structures were elucidated as 3β, 12β, 20S‐trihydroxy‐25‐methoxydammar‐23‐ene 3‐O‐{[β‐D‐glucopyranosyl(1→2)‐β‐D‐glucopyranosyl]‐20‐O‐[α‐L‐arabinopyranosyl(1→6)]‐β‐D‐glucopyranoside (1) and 3β, 20S‐dihydroxy‐12β, 23R‐epoxydammar‐24‐ene 3‐O‐{[β‐D‐glucopyranosyl(1→2)‐β‐D‐glucopyranosyl]‐20‐O‐[β‐D‐xylopyanosyl(1→6)]‐β‐D‐glucopyranoside (2) on the basis of 1D and 2D NMR (including 1H, 13C‐NMR, 1H1H COSY, HSQC, TOCSY, HMBC, and NOESY), ESI‐MS spectrometry and chemical methods. Copyright © 2008 John Wiley & Sons, Ltd.
Home healthcare practice environment: Predictors of RN satisfaction
Authors: Tullai‐McGuinness Susan 
Published: May 2008

AbstractSince 2000, Medicare‐certified home healthcare agencies have undergone significant regulatory changes that directly affect registered nurses. The satisfaction of 201 nurses from 19 home healthcare agencies was examined. Home healthcare nurses reported slightly lower satisfaction than hospital nurses and were approximately 50% less satisfied than they were in 2000. Satisfaction had a negative relationship with years worked as a home healthcare nurse (r = −.25, p < .01). Controlling for years of home healthcare experience, control over practice decisions and practice setting decisions were significant predictors of satisfaction. With the demand for home healthcare nurses expected to increase 109% by 2020, development of a National Database of Nursing Quality Indicators for home healthcare that includes satisfaction as a nurse‐sensitive outcome might be helpful. © 2008 Wiley Periodicals, Inc. Res Nurs Health 31:252–260, 2008