bluuebunny/crossref_metadata_2025_split huggingface dataset:
The association between loneliness and mental health disorders in older people after the COVID-19 pandemic
Authors: Araújo Odete , Sousa Lia , Sampaio Francisco , Sequeira Carlos , Teixeira Laetitia 
Published: March 2024

Abstract

Background
Acknowledging the exacerbated impact of the COVID-19 pandemic on the mental health status of the general population, particularly the older population, this study aimed to explore the association between loneliness and mental health disorders in older people after the COVID-19 pandemic.
Methods
A cross-sectional survey using the SHARE database was conducted from June to August 2020 amid the COVID-19 pandemic.
Results
The sample comprised 847 participants, including 393 (46.4%) men, with an overall mean age of 74.6 years (sd = 6.7 years). Concerning mental health indicators, 441 (52.1%) reported feelings of nervousness, 384 (45.3%) experienced sadness or depression, 349 (41.2%) encountered sleeping difficulties, and 280 (33.1%) reported experiencing loneliness often or some of the time. The results revealed increased loneliness feelings for women who had worse health, increased fear of falling, dizziness, heightened fatigue, anxiety, depression, and concurrent health and sleep problems. Notably, age and medication had no impact on feeling lonely.
Conclusions
The pandemic has adversely affected the mental well-being of older population. These findings provide valuable insights for the development of targeted strategies to prevent mental health problems.
Unraveling the tumor-promoting role of wildtype Isocitrate dehydrogenase 1 (IDH1) in human gliomas
Authors: Li Xiang , Tao Yiran , Lyu Yuan , Li Junqi , Liang Wulong , Liu Wanqing , Hu Nan , Jing Zhou , Li Zian , De Xiao , Dai Lirui , Zheng Yuqian , Shi Zimin , Hu Weihua , Zhou Shaolong , Shan Qiao , Fu Xudong , Wang Xinjun 
Published: March 2024

Abstract

Isocitrate dehydrogenase 1 (IDH1) mutations are discovered in most grade Ⅱ gliomas (71%-78%), grade Ⅲ gliomas (62%-78%) and secondary glioblastomas (88%), and have received lots of attention in recent years. However, the tumor-promoting role of wildtype IDH1 still need to be further investigated. In this article, we found wildtype IDH1 mRNA and protein levels were both elevated in glioma by using bioinformatic analysis, Besides, IDH1 mutation reduced the expression of wildtype IDH1 in U87-R132H cell line. Furthermore, the expression of wildtype IDH1 also increased along with the increase of clinical grades of glioma. Cell function and signaling pathways enrichment analyses were enriched in metabolic processes, phosphatase complex, TCA, DNA replication, p53 signaling pathway, Notch signaling pathway, et al. Single-cell sequencing analysis revealed that high expression of wildtype IDH1 correlated with cell cycle, metastasis, EMT, proliferation, invasion, stemness, and DNA damage. Besides, wildtype IDH1 promoted GBM cell viability, migration, and radioresistance in vitro. Wildtype IDH1 was significantly relevant with diagnosis, prognosis, and survival probability of glioma patients. Therefore, wildtype IDH1 could be an underlying target for glioma therapy.
Accurate Method for Estimating Wall-Friction based on Analytical Wall-Law Model
Authors: Zhou Lei , Wang Duo , Cao Bochao , Xu Hongyi 
Published: March 2024

Abstract

A novel method is proposed for accurately determining the local wall friction through the near-wall measurement of time-average velocity profile in a Type-A turbulent boundary layer (TBL). The method is based on the newly established analytical wall-law in Type-A TBL. The direct numerical simulations (DNS) data of turbulence on a zero-pressure-gradient flat-plate (ZPGFP) is used to demonstrate the accuracy and the robustness of the approach. To verify the reliability and applicability of the method, a two-dimensional particle image velocimetry (PIV) measurement was performed in a ZPGFP TBL with the low-to-moderate Reynolds number (Re). Via utilizing the algorithm of single-pixel ensemble correlation (SPEC), the velocity profiles in the ZPGFP TBL were resolved at a significantly improved spatial resolution, which greatly enhanced the measurement accuracy and permitted to accurately capture the near-wall velocity information. The accuracy of the approach is then quantitatively validated for the high Reynolds number turbulence by the ZPGFP TBL data. The research demonstrates that the current method can provide the precise estimation of wall friction with a mean error of less than 2%, which not only possesses the advantage of its insensitivity to the absolute wall-normal distance of the measuring point, but also is capable of providing an accurate prediction of wall shear stress based on fairly sparse experimental data on the velocity profile. The current study demonstrates that the wall shear stress can be accurately estimated by a velocity even at a single-point either measured or calculated in the near-wall region.
A novel posterior keratoconus with osteogenesis imperfecta by mutation in COL1A1
Authors: Wu Hui , Shi Xiaoru , Shi Hui , Liu Lei , Xu Hui , Hou Lulu , Huang Rong , Wang Chunmei , Jia Hui 
Published: March 2024

Abstract

BackgroundPosterior keratoconus is a rare, non-progressive corneal condition in the posterior surface of the cornea. Also known as keratoconus posticus, it is characterized by thinning of the posterior cornea without ectasia of the anterior cornea. Osteogenesis imperfecta (OI) is a rare inherited connective tissue disease affecting collagen production. The manifestations in OI patients have not been well studied in the case of keratoconus, especially posterior keratoconus. Here, we observe that a clinical patient has the syndrome of OI caused by mutations in the COL1A1 genes and Descemet membrane detachment.Case presentationWe report a 27 years old Chinese male with skeletal deformity and hirsutism complained of blurred vision in his left eye for one month. He had hearing loss in his both ears 6 years ago. There were many times of bone fragility history. Cardiac ultrasound indicated cardiac insufficiency. On examination, best corrected visual acuity (BCVA) was 20/40 in the right eye and light perception in the left eye. Slit lamp examination of the left eye showed corneal edema, paracentral corneal thinning without ectasia of the anterior cornea and a peripheral circular band of corneal opacity which was sharply demarcated. Examination of the right eye was normal except a peripheral circular band of corneal opacity as the same as the left eye. Topographic analysis of the cornea has further shown that there are in fact anterior surface changes in posterior keratoconus, including paracentral steepening in the area of the posterior corneal depression with gradual peripheral flattening. Ultrasound biomicroscopy has been shown the local anterior bulging of the posterior corneal surface, thinning of the superior stroma, a central Descemet’s detachment and enhanced stromal reflectivity corresponding to the stromal opacity seen in circumscribed posterior keratoconus. The diagnosis of posterior keratoconus accompanied with bilateral peripheral circular corneal degeneration was made and the patient underwent penetrating keratoplasty of the left eye. After the operation, both anatomic and visual development success were obtained. However, the patient was suffering from overall dysplasia. We speculated that it might be a systemic syndrome. So we performed gene testing of blood samples from patient and his family members after obtaining their informed consent. The results indicated a mutation in the COL1A1 gene (NM_000088.4:exon7:c.572_573 delins AA: p.G191E).ConclusionsThis is the first report which covers posterior keratoconus occurring in patient with OI. This novel report reminds us the importance of taking eye examinations into consideration in systemic syndrome.
Influence of pores on the fatigue life of welded joints and investigation of acceptance criteria defined in standard documents
Authors: Jung Matthias , Fenzl René 
Published: March 2024

Abstract

Inner defects in welded joints constitute concurrent sites for fatigue failure in welds, especially when high fatigue resistance is enforced at weld toe and root. Existing standards limit the size of pores and other inner defects proportional to the sheet or weld thickness, but for larger dimensions cutoff values are in place. This leads to overly high acceptance criteria when it comes to thicker sheets, as e.g. used for wind turbine towers. To analyze the influence of pores on the fatigue life, specimens with targeted pore sizes larger than the usually accepted were tested under fatigue loading. In the present study a numerical model for fatigue assessment was developed based on the experimental results, which allowed to further vary pore sizes and locations. It is shown, that for larger weldments the acceptable pore size can be increased without large reductions in fatigue life. But, the location of the pore with regard to the specimens surface has shown to have a large influence – an effect that is currently not considered in common weld quality documents.
IIW Document No.: XIII-2994-2023
Molecular and biological characterization of infectious full- length cDNA clones of two viruses in Paris yunnanensis, including a novel potyvirus
Authors: Lan Pingxiu , He Peng , Mu Aiqiu , Cao Mengji , Wang Yun , Zhou Guohua , Chen Xiaojiao , Cai Hong , Li Fan 
Published: March 2024

Abstract

Paris yunnanensis, also named as Rhizoma Paridis in the Chinese Pharmacopeia, is a perennial Chinese medicinal herb commonly grown in Southwest China. In recent years, this plant has been found to be infected with many viruses. Using high-throughput sequencing (HTS) and Sanger sequencing, we obtained the complete genome sequences of three capillovirus isolates and one potyvirus isolate. Genomic and phylogenetic analysis results revealed that these three capillovirus isolates and the Paris polyphylla chlorotic mottle virus, a newly reported capillovirus, are the same virus belonging to the genus Capillovirus. The newly found potyvirus isolate shares 52.4-68.9% nucleotide sequence identity with other known potyviruses and thus, is grouped into the bean common mosaic virus subgroup. Based on the nucleotide sequence identity, we consider this virus is a novel potyvirus species and name Paris potyvirus 5 (ParPV-5) as its common virus name, Potyvirus shilinense as its species name. To characterize their biological features, two infectious clones, representing the two viruses, have been constructed through homologous recombination or yeast homologous recombination, respectively. Agrobacterium-mediated inoculation assay results showed that the Paris polyphylla chlorotic mottle virus (PpCMV) can infect P. yunnanensis, Nicotiana benthamiana, N. tabacum var. Xanthi nc, Cucurbita moschata, and Capsicum annuum, while the potyvirus can infect N. benthamiana, Cucumis sativus L., and Bidens pilosa L. A total of 179 field P. yunnanensis leave samples from four counties were detected using RT-PCR, the result showed that ParPV-1 is uncommon in Paris yunnanensis but PpCMV is an preponderant species in some surveyed regions.
Development and externally validated prediction model of individualization of FSH starting dose in the depot GnRH agonist protocol for the early follicular phase
Authors: Fan Wenqian , Ye Tian , Du Linqing , Tian Lifeng , Kong Huijuan 
Published: March 2024

Abstract

Background
Each controlled ovarian hyperstimulation(COH) protocol has its own unique mechanism and hormone pattern. The depot GnRHa protocol has a deeper down-regulation effect and favourable clinical pregnancy rates, the predictive model of the optimal follicle-stimulating hormone (FSH) starting dose in the early follicular phase depot GnRH agonist (EFDGa) protocol has not been reported. Our study was made to explore predictive indicators for determining the optimal FSH starting dose in patients undergoing ovarian stimulation with the EFDGa protocol in assisted reproductive technology (ART), and to develop and validate a nomogram prediction model for the starting dose of FSH.
Methods
This retrospective study included 2733 cycles who underwent fresh cycle transplantation at two large teaching hospitals in China from January to December 2022: center 1 (Reproductive Medicine Center of first affiliated Hospital of Zhengzhou University) provided the data for modelling (n = 938) and internal testing (n = 400), and center 2 (Reproductive Medicine Center of Jiangxi Maternal and Child Health Hospital) provided the data for external testing (n = 1109). Patient demographics, including age, anti-Mullerian hormone (AMH) levels, baseline endocrine profile, and body mass index (BMI), along with information on ovulation stimulation, were collected. Univariate and multivariate linear regression models were used to identify factors influencing the FSH starting dose. A nomogram for the ideal FSH starting dose was developed based on these factors and validated internally and externally. Bland and Altman plots and paired t-tests were conducted to verify the concordance and RMSE between groups.
Results
Univariate analysis revealed that patient age, BMI, baseline FSH, AMH, and antral follicle count (AFC) were indicators of FSH starting dose. The regression model for predicting FSH starting dose was determined as: Initial dose of FSH = 45.984 + 1.728 * AGE (years) + 5.131 * BMI (kg/m²) + 2.455 * bFSH (IU/ml) − 6.697 * AMH (ng/ml) – 3.339 * AFC. Bland and Altman analysis showed good agreement in the internal validation (bias: 0.629, SD of bias: 36.83, 95%LoA: -71.55–72.81 IU). Furthermore, validating the model on external cohort confirmed that nomogram prediction model is an accurate predictor of FSH starting dose ((bias: -1.428, SD of bias: 43.21, 95%LoA: -85.11–82.15 IU).
Conclusions
We established a model for effectively predicting the ideal FSH starting dose, with the nomogram model providing an intuitive representation of the data. The predictive model demonstrates practical utility, effectively initiating a proper ovarian response and preventing adverse ovarian reactions or the occurrence of ovarian hyperstimulation syndrome. As more IVF cycles are being generated in the future, this model will be valuable in clinicians using basic parameters to assess proper initial dose of FSH.
Outcomes of Video-Assisted Thoracoscopic Wedge Resection Using a Non-Crushing Clamp and Continuous Sutures Plus Hemovac Drains Versus Staplers Plus Conventional Chest Tube: A Comparative Study
Authors: Fazlıoğlu Mithat 
Published: March 2024

Abstract
OBJECTIVES.
In thoracoscopic surgery, we described wedge resection of the parenchyma with clamps and subsequent use of hemovac drains and compared it with the standard technique of using staplers and conventional chest tubes.
METHODS.
Fifty-nine patients who underwent thoracoscopic wedge resection between 2018 and 2024 were retrospectively analyzed. Patients were divided into two groups according to the surgical and drain types: stapler plus chest tube (S-ct, n = 27) and clamp plus hemovac drain (C-hd, n = 32). Air leak duration, postoperative drainage time, hospital stay duration, and total medical costs were also investigated.
RESULTS.
There were no significant differences in patient characteristics between both groups. The operation time was longer in Group C-hd (median 70 min versus median 60 min, p = 0.115). Prolonged air leak rates were similar between the two groups (11.1% and 12.5% in Groups S-ct and C-hd, respectively). The air leak duration and postoperative drainage time were not statistically significantly different between both groups (p = 0.872 and p = 0.176, respectively). The VAS scores on the first postoperative and day were significantly lower in Group C-hd (p = 0.02, and p = 0.02, respectively). Pain requiring additional treatment was significantly higher in Group S-ct (33.3% vs. 9.4%, p = 0.04). Total medical cost was statistically significantly higher in Group S-ct (median 371.7 vs. 191.6 dollars, p < 0.001).
CONCLUSION.
In thoracoscopic surgery, wedge resection of the parenchyma with clamping and subsequent use of a hemovac drain are comparable to the standard technique of using staplers and conventional chest tubes regarding short-term results and better economic results. Hemovac drainage can reduce postoperative pain.
The combination of the 18F-FDG and 18F-FP-DTBZ PET for early diagnosis of cerebral glucose metabolism and dopaminergic dysfunction in Parkinson's disease
Authors: li shuang , Lu Jie , Lu Wei-Zhao , Song Tian-Bin , Zhang Chun , Yang Chang 
Published: March 2024

Abstract

Objective: This study aimed to use 18F-FDG and 18F-FP-DTBZ PET to illustrate the correlation between cerebral glucose metabolism and nigrostriatal dopamine function and to investigate the precision of early Parkinson's disease (PD) diagnosis.
Methods: 18 healthy control participants and 32 patients with early PD were recruited for 18F-FDG and 18F-FP-DTBZ PET scans.
Results: Patients with PD exhibited significant 18F-FDG metabolic reduction in the frontal, parietal, and temporal cortex, as well as notable increased metabolism in the putamen, thalamus, pons, and cerebellum (all P < 0.05). Additionally, there was a notable reduction in 18F-FP-DTBZ uptake in the bilateral caudate, anterior putamen, and posterior putamen (all P < 0.005). The reduction of 18F-FP-DTBZ uptake in the bilateral caudate and anterior putamen positively correlated with the reduction in 18F-FDG uptake in the frontal cortical regions (r=0.352, 0.324, 0.300, and 0.314, respectively; all P < 0.05) and the parietal cortical regions (r=0.329, 0.303, and 0.330, respectively; all P < 0.05). The combination of the 18F-FDG and 18F-FP-DTBZ model exhibited the highest reliable prognostic performance, yielding an area under the curve of 0.979 (95% CI [0.948–1.00], P < 0.01).
Conclusions: Our study suggested that 18F-FDG and 18F-FP-DTBZ PET imaging was an excellent tool in differentiating PD from normal aging in the early stage of the disease.
Pharmacological activation of Sig-1R ameliorates pathological neuroinflammation in rats with diabetic neuropathic pain via the Akt/GSK-3β/NF-κB pathway
Authors: Cao Shan-shan , Shi Lei-lei , Zhang Yu-han , Shi Yong , Wang Xin , Yuan Shi-yu , Han Chao-jun , Liu Ji-ping 
Published: March 2024

Abstract
Diabetic neuropathic pain (DNP) is a common complication of diabetes mellitus (DM) and is characterized by spontaneous pain and neuroinflammation. The Sigma-1 receptor (Sig-1R) has been proposed as a target for analgesic development. It has anti-inflammatory properties and has been found to regulate DNP. However, it is not known whether Sig-1R can ameliorate pathological neuroinflammation in DNP. The present study used a rat model of DNP and a highly selective agonist of Sig-1R to assess the effects of the protein on neuropathic pain in rats with type 2 diabetes mellitus. The rats were divided into Control, Model, PRE-084 (0.3 mg/kg), PRE-084 (0.6 mg/kg), PRE-084 (1 mg/kg), and metformin (Met, 20 mg/kg) groups, with seven rats per group, and their body weight, fasting blood glucose, mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were tested weekly for two weeks. After treatment with PRE-084, the pain thresholds in the DNP rats were significantly improved, together with pathological changes in the dorsal root ganglion, reductions in the serum levels of TNF-α, IL-1β, IL-6, MOD, and prostaglandin E2, and the activity of superoxide dismutase was increased. The mRNA levels of TNF-α, IL-1β, and cyclooxygenase 2 were reduced. Pharmacological inhibition of Sig-1R with BD1047 (10 µM) abolished Sig-1R-mediated activation of lipopolysaccharide-treated BV-2 microglial cells. It was also found that PRE-084 increased phosphorylation of serine/threonine protein kinase B (Akt) and glycogen synthase kinase 3β (GSK3β) at Ser9, inhibiting nuclear factor kappa B(NF-κB)-mediated neuroinflammation in the dorsal root ganglion, thus reducing DNP. The findings suggest that the effect of Sig-1R agonist PRE-084 on DNP may be to reduce the level of inflammation by downregulating Akt/GSK-3β/NF-κB signaling, thereby contributing to the treatment of the disease.
An aid diagnostic platform to detect the transition of mild cognitive impairment (MCI) to Alzheimer's disease (AD) based on 48,116 AD and MCI patients
Authors: Song You-Qiang , LI JING , Li Siwen , Shea Yat-fung , Yue Ming , Zhu Pengfei , Zou Quan , Yuan Shuofeng , Chu Leung-Wing 
Published: March 2024

Abstract
Alzheimer's disease (AD) is an incurable, progressive neurodegenerative disorder, necessitating early diagnosis and intervention. Mild cognitive impairment (MCI) often precedes AD, but not all cases progress to AD, emphasizing the need for predictive biomarkers. We analyzed routine blood test data from 43,981 AD patients and 4,537 MCI subjects in Hong Kong hospitals (2000-2019). Among 31 shared biomarkers, five blood biomarkers (Hemoglobin, Hematocrit, Red blood cell related to oxygen carrying capacity, Neutrophils, and White blood cell related to immunity) significantly differentiated MCI from AD. Subjects were divided into four groups (Female 65 ~ 74, Male 65 ~ 74, Female 75 ~ 89, Male 75 ~ 89) to minimize gender and age bias. Models utilizing the five biomarkers along with machine learning yielded the highest accuracy in the Female 65 ~ 74 group (AUC of 0.76 on an independent test set). The other three models were trained with other biomarkers besides these 5 to optimize predictions, capturing models with AUC close to 0.70. We then constructed a platform predicting the risk of MCI converting to AD (MAP, http://lab.malab.cn/~lijing/MAP.html) to help physicians and MCI subjects with early diagnosis and prevention of AD. In conclusion, this study demonstrates the potential for accurate prediction of MCI to AD conversion using routine blood test data and machine learning, offering an economical and practical approach for early AD screening in MCI individuals.