bluuebunny/crossref_metadata_2025_split huggingface dataset:
Producing recombinant proteins in Vibrio natriegens
Authors: Smith Matthew , Hernández José Sánchez , Messing Simon , Ramakrishnan Nitya , Higgins Brianna , Perkins Shelley , Cregger Julia , Le Phuong Vi , Wall Vanessa , Grose Carissa , Mehalko Jennifer , Johnson Adam , Frank Peter , Sherekar Mukul , Pagonis Morgan , Widmeyer Stephanie , Denson J-P , Snead Kelly , Drew Matt , Hong Min , Poon Ivy , Waybright Timothy , Champagne Allison , Esposito Dominic , Jones Jane , Taylor Troy , Gillette William 
Published: April 2024

Abstract

The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system. The nature of the target proteins themselves, along with cost, ease of use, and speed, are typically cited as major factors to consider in production. Despite a wide variety of alternative expression systems, most recombinant proteins for research and therapeutics are produced in a limited number of systems: Escherichia coli, insect cells, and the mammalian cell lines HEK293 and CHO. Recent interest in Vibrio natriegens as a new prokaryotic recombinant protein expression host is due in part to its short doubling time of <10 minutes but also stems from the promise of compatibility with techniques and genetic systems developed for E. coli. We successfully incorporated V. natriegens as an additional prokaryotic expression system for recombinant protein production and report improvements to published protocols as well as new protocols that expand the versatility of the system. While not all proteins benefit from production in V. natriegens, we successfully produced several proteins that were difficult or impossible to produce in E. coli. We also show that in some cases, the increased yield is due to higher levels of properly folded protein. Additionally, we were able to adapt our enhanced isotope incorporation methods for use with V. natriegens. Taken together, these observations and improvements allowed production of proteins for structural biology, biochemistry, assay development, and structure-based drug design in V. natriegens that were impossible and/or unaffordable to produce in E. coli.
Warfarin-Related Nephropathy: Unveiling the Hidden Dangers of Anticoagulation
Authors: xufengbo 徐丰博 , Wang Guoqin , Sun Lijun , Cheng Hong 
Published: April 2024

Abstract
Warfarin-related nephropathy (WRN) is defined as acute kidney injury (AKI) subsequent to excessive anticoagulation with warfarin. Patients with mechanical prosthetic valves required long-term anticoagulant therapy, and warfarin remains the sole available option for anticoagulant therapy. Consequently, patients with mechanical prosthetic valves constitute a special group among the entire anticoagulant population. The present study recorded two cases in which patients who had undergone mechanical prosthetic valve surgery and were receiving warfarin therapy presented to the hospital with gross hematuria and progressive creatinine levels. Notably, the international normalized ratio (INR) did not exceed 3 in these two patients. Subsequent renal biopsies confirmed the presence of WRN with IgA nephropathy. The two patients continued to receive anticoagulation with warfarin and were prescribed oral corticosteroids and cyclophosphamide, which resulted in improved renal function during the follow period. Based on a review of all relevant literature and the present study, we also pose a new challenge, whether elevated INR levels must be as one of the criteria for clinical diagnosis of WRN? Perhaps it can get some inspiration from the present article.
Transurethral resection of the prostate (TURP) versus Rezūm for benign prostatic hyperplasia (BPH): prospective and comparative study
Authors: Yushanjiang Suliya , Qu Rui , Zhao Meng , Chen Guo , Yang Hui , Zhao Yue , Dai Yi , Yang Luo 
Published: April 2024

Abstract

Background
Benign prostatic hyperplasia(BPH) is the common male conditions that cause lower urinary tract symptoms disturbing patients. The treatments include pharmacologic, conventional transurethral resection of the prostate(TURP), and minimally invasive. Rezūm as a minimally invasive method has proven effective for BPH, but there is limited comparison with existing treatment modalities. Therefore, we conducted a comparison between Rezūm surgery and the TURP to assess the safety and efficacy of Rezūm.
Methods
Patients hospitalized at the West China Fourth Hospital for BPH were enrolled according to inclusion and exclusion criteria, with 20 undergoing TURP surgery and 20 undergoing Rezūm surgery. After one year of follow-up, we gathered comprehensive patient demographics and perioperative data and conducted postoperative evaluations including the International Prostate Symptom Score (IPSS) and Quality of Life (QOL) scores.
Results
After one year of follow-up, IPSS scores decreased by 57.35% in the TURP group and by 61.50% in the Rezūm group, respectively (p < 0.05). Similarly, QOL scores decreased by 63.12% and 63.92% in each respective group (p < 0.05). Notably, the Rezūm group exhibited significantly shorter operative times, reduced operative blood loss, and shorter hospital stays compared to the TURP group (p < 0.0001, p < 0.0001, p = 0.025). Three days post-surgery, the Rezūm group reported lower Numeric Rating Scale (NRS) scores compared to the TURP group (p < 0.0001), and experienced a shorter duration of hematuria (p = 0.023).
Conclusions
Rezūm enhanced the efficacy of BPH treatment at the one-year postoperative mark, yielding outcomes on par with those of TURP. Furthermore, Rezūm showcased notable advantages during the perioperative phase.
Optimizing two stage chemical Pretreatment for Enset ventricosum: A Response Surface Methodology Approach to Enhance Bioethanol Production Efficiency
Authors: Delisho Fitsum Dejene , Gedefaw Yeayneabeba Molla , Aba Dawit Albenea , Woldemichael Teketelew Belete 
Published: April 2024

Abstract
Background: Bioethanol, a promising alternative to fossil fuels, effectively mitigates CO2 emissions, reduces global warming, and helps combat urban pollution while promoting sustainable energy solutions. Therefore, the objective of this study is to optimize a two-stage chemical pretreatment for Enset ventricosum in order to enhance the efficiency of bioethanol production.
Results: In the experiment, Enset biomass leaves were subjected to a two-stage treatment using a solution of 1% H2SO4 and 1% NaOH (acid-alkaline treatment). The acid-alkaline pretreatment yielded the highest bioethanol content of 5.2 mg/ml and sugar concentration of 6.2 mg/ml, whereas the untreated sample had a bioethanol concentration of 2.2 mg/ml and sugar concentration of 3.4 mg/ml. The analysis using Response Surface Methodology identified optimal synthesis parameters for the pretreatment. These included a biomass concentration of 19.9775%, a pH of 3.86387, a temperature of 32.5739 °C, and an incubation period of 71.0274 hr. These parameters led to a predicted bioethanol yield of 9.65332 mg/ml with a desirability score of 1.000. Further analysis using SEM revealed significant changes in the surface morphology of the pretreated biomass. Additionally, FTIR spectroscopy confirmed the presence of cellulose and hemicellulose in the feedstock.
Conclusion: These findings demonstrate the potential of Enset ventricosum biomass as a sustainable feedstock for bioethanol production. The study also highlights the importance of two stage chemical Pretreatment and parameter optimization in enhancing bioethanol yield.
Adsorption of OTC from Aqueous Solution by UiO-66-NH2: Kinetics, Isotherms, and Thermodynamics
Authors: He Hao , Liu Xiao-Kai , Zhao Bai-Yun , Zhou Li-Juan , Zhao Xuan , Wang Chen-Xu , Zhang Ji-Yuan , Zhang Yuan-Fang , Wang Li 
Published: April 2024

Abstract
In this study, UiO-66-NH2 nanoparticles were synthesized by a hydrothermal method, and subsequently, the adsorption performance of UiO-66-NH2 on oxytetracycline (OTC) was evaluated by batch adsorption experiments, in which the key parameters, such as the concentration of OTC, pH, reaction time, and temperature, were investigated. UiO-66-NH2 nanoparticles were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), BET specific surface area, and transmission electron microscopy (TEM). The results showed that the adsorption data conformed to the Sips model, indicating a maximum adsorption capacity of 322.48 mg·g− 1. Additionally, the quasi-primary kinetic equilibrium adsorption capacity was determined to be 314.61 mg·g− 1, while the quasi-secondary kinetic equilibrium adsorption capacity reached 365.69 mg·g− 1. The adsorption kinetics suggested that the process was influenced by physicochemical co-actions. Notably, the specific surface area of UiO-66-NH2 was measured at a high value of 998.663 m²·g⁻¹, potentially contributing to improved adsorption efficiency. Analyses using FT-IR, XRD and XPS confirmed the adsorption of OTC on the material surface. Overall, the main driving forces behind the adsorption process are weak electrostatic interactions, hydrogen bonding interactions and π-π stacking interactions.
The Role of Medication Literacy and Polypharmacy in Sarcopenia Among Maintenance Hemodialysis Patients: A Cross-Sectional Analysis
Authors: Zhu Linfang , Liu Yang , Yang Fengxue , Li Jie , Yuan Huaihong , Fu Ping 
Published: April 2024

Abstract
Purpose
Sarcopenia is significantly prevalent among maintenance hemodialysis patients, with the contributing factors of medication literacy and polypharmacy receiving limited exploration in current research. This study aims to fill this gap by assessing the impact of these factors, along with demographic and malnurtition, on sarcopenia risk.
Methods
Conducted at the Wenjiang Hemodialysis Center in West China Hospital, this descriptive cross-sectional study involved 236 participants. Data collection included the Chinese Medication Literacy Scale, Malnutrition-Inflammation Score assessments, bioelectrical impedance analysis, and grip strength measurements, with sarcopenia diagnosed according to the 2019 AWGS criteria.
Results
The study included 236 participants. Of these, 87 (36.9%) had no sarcopenia, 121 (51.3%) were pre-sarcopenia, 7 (3.0%) were sarcopenia, and 21 (8.9%) had severe sarcopenia. Ordinal logistic regression analysis identified male gender (OR = 0.557, 95% CI: 0.322 to 0.962, P = 0.036), age below 65 (OR = 0.178, 95% CI: 0.082 to 0.389, P < 0.001), and good nutritional status (OR = 0.544, 95% CI: 0.310 to 0.954, P = 0.034) as protective against severe sarcopenia. Conversely, the combination of polypharmacy and limited medication literacy (OR = 1.956, 95% CI: 1.094 to 3.496, P = 0.024) was significantly associated with an increased risk of sarcopenia progression.
Conclusion
The study highlights the protective role of good nutrition and the lesser susceptibility of males and younger individuals to severe sarcopenia. It underscores the necessity of targeted interventions to address the compounded risk presented by polypharmacy and limited medication literacy in patients undergoing hemodialysis.
The Champati Slide
Authors: Pudasaini Shiva P. , Dangol Bekha Ratna , Tiwari Chet Nath , Kafle Jeevan , Pokhrel Puskar R. , Kattel Parameshwari 
Published: April 2024

Abstract
Here, we report some novel findings of chute experiments with native Nepalese granular seeds, called Champatis, and when mixed with other food grain of a fundamentally different physical properties, Silam. The epitomic supergrain Champati exhibits complex frictional, spin and rolling motion. We hypothesize that the Champati slide results in an essentially unique dynamical spreading, separation from other grains, mobility, run-out and deposition morphology. Particularly, the surface anatomy of Champati acted vitally in characterizing these aspects. We reveal spectacular dynamical flow obstacle interaction and depositional behaviour of Champati and the mixture slide. Champati slide manifests hyper-spreading in the run-out. Soon after the mass hits the ground, the behaviour is unprecedented and unpredictable, mainly around the frontal periphery. The very special properties of Champatis control the frontal spreading, rapid grain marching and the zigzag sporadic Champati motion. Particle rolling, spinning, exceptionally lower frictional energy dissipation and grain collision played the major role resulting in an unexpectedly longer travel time and distance, explaining the astonishing hypermobility of Champati grains. The obstacle substantially changes the flow dynamics as the Champati has the higher object mobilization capacity. Due to the unique property of the supergrain, the eye-catching, amazingly strong separation of Champati from Silam evolves swiftly right after the flow inception, and the process intensifies quickly. The separation length characterizes the complex interfacial momentum exchange between the phases in the mixture. The separation length between the frontal Champatis and Silam grains increases exceptionally as it does between the main body and the exclusively Silam-covered tremendously long tail of the flow in the inclined portion of the channel. These results may be useful in better understanding the phase separation, super-wide-spreading and hypermobility of some geological flows, including fragmented rock avalanches, probably helping resolve some relevant standing challenges.
Microbial communities associated with the nursery stage of commercially cultivated seaweed Saccharina japonica in Southern China
Authors: Zhuang Yingrui , Saha Mahasweta , Egan Suhelen , Han Yixiao , Qiu Qiying , Chen Defu , Wang Gaoge 
Published: April 2024

Abstract
Epimicrobiota associated with seaweeds are crucial for the health and development of their hosts due to their ability to produce phytohormones and vitamins etc. However, there is limited knowledge related to the microbiota of commercially cultivated seaweed Saccharina japonica. In this study, we investigated the dynamics of microbiota associated with S. japonica at nursery stage using Illumina sequencing of the V3-V4 hypervariable region of 16S rRNA gene. The composition and structure of epimicrobiota showed significant differences at the transition time (from mature sporophytes to sporelings). While, the epimicrobiota were relatively stable during the development of sporelings. Blastopirellula and Pseudoalteromonas were the dominant genera of the community of mature sporophytes and 6-week-old sporelings, respectively. Rubritalea was the most dominant genus for both 7 and 8-week-old sporelings. These three genera were also part of the core microbiota, suggesting that they may play an essential function within the S. japonica holobiont. In addition, members of the Planctomicrobium and Roseibacillus were identified as both drivers and keystone species, which might be responsible for the epimicrobiota shifts from 7-week-old sporelings to 8-week-old sporelings and were fundamental for the newly assembled epimicrobiota. Our results enrich the baseline data related to the microbiota of the commercially cultivated S. japonica.
Advancements and Comparative Analysis of Real-Time Face Recognition Systems
Authors: Saidakhmatov Mirolim 
Published: April 2024

Abstract
This paper investigates diverse face recognition methodologies within centralized computer vision systems, encompassing traditional techniques, deep learning frameworks, and cloud-based solutions. It explores optimization strategies aimed at enhancing model performance and efficiency. Through rigorous evaluation and comparative analysis, the study elucidates the strengths and limitations of each approach, providing valuable insights for practitioners and researchers. The findings contribute to the advancement of face recognition technology, offering guidance for future research endeavors and practical implementations in various real-world applications.
Energy Management Method of Integrated Energy System Considering Multi-mode Energy Storage and User Regulation Characteristics
Authors: Enbo Luo , Hai Lu , Yuanlong Zhang , Hao Zhang , Jiyong Xu , Guo Jie , Li Yan , Huang Yujing 
Published: April 2024

Abstract

With the development of multi-energy system and energy storage industry, the energy system is more diversified, and the research on its integration and complementarity is of great significance. First of all, the system model of users and building including multi-energy storage is established. The energy storage in the user model includes electric vehicle and building virtual energy storage. While considering human behavior and user comfort, the uncertainty model of human behavior based on conditional trigger is established. The energy storage in the building model includes actual energy storage devices such as batteries and ice storage tank. Then, a stackelberg game model is established, in which the user is the follower and takes the lowest cost as the goal to respond to the demand. As the leader, the building operator adjusts the energy price according to the result of the user response, with the goal of maximizing its own profit. The distributed algorithm is used to solve the problem and iterate to the game equilibrium repeatedly. Finally, a numerical example is given to verify the effectiveness of the proposed model and algorithm, and analyze the impact of human behavior on the virtual energy storage system, as well as the differences between generalized energy storage systems and the impact of strategies.
Fly-Ash based Flame-Retardant Cellulose Materials for Strengthening and Value-Added Utilization in Industrial Solid Wastes
Authors: He Wentao , Tan Lei , Wu Yongjia , Wei Yongchun , Chen Yiyang , Li Dan , Wang Guxia , Qian Yongqiang , Guo Shengwei 
Published: April 2024

Abstract

Cellulose, a bio-based material, is increasingly researched and valued for its abundant availability and exceptional characteristics. However, Cellulose has a flammable problem. This study addresses this issue by integrating it with industrial waste fly ash (FA) to overcome its natural flammability. By solution compounding, the study successfully developed cellulose/FA films and porous structures, significantly boosting the material's flame-retardant capabilities. This innovation not only enhances the practical application of cellulose but also promotes the high-value reuse of FA, resonating with the principles of sustainable development. The cellulose/FA hydrogel, characterized by a homogeneous and stable blend of FA particles and cellulose, achieves this through effective affinity and hydrogen bonding, ensuring optimal miscibility and encapsulation. In terms of thermal properties, the modified composites (C-F10, C-F20 and C-F30) demonstrate a substantial increase in initial decomposition temperatures, approximately 26℃ higher than pure cellulose, ranging between 282℃ and 302℃. This enhancement is attributed to the formation of an inorganic protective layer on the cellulose matrix, which significantly improves thermal stability while maintaining key mechanical properties. Remarkably, the flame retardancy of these materials shows notable improvement, particularly at a 30wt% FA concentration, with the limiting oxygen index (LOI) of the porous and film structures reaching around 29% and 31%, respectively. This advancement greatly elevates their flame resistance. Overall, this study presents a pioneering approach in developing eco-friendly, flame-retardant materials by repurposing industrial waste, marking a significant stride in sustainable material innovation.
Optimizing the Video Streaming Performance with an Energy-Efficient Evolution of Cub to Predator Based Resource Allocation
Authors: P Subotha S , L Femila , M Swamy S , R Valarmathi I 
Published: April 2024

Abstract

Wireless sensor networks (WSNs) and video streaming services have gained popularity in recent years. The MPEG-4 H.264/AVC protocol has been widely adopted for video streaming in WSNs, but it is important to transmit sensitive video packets securely to protect the confidentiality of mission-critical applications. It is crucial to ensure that security measures do not negatively impact network performance or video quality. While previous research has focused on secure and energy-efficient video streaming over WSNs, none have addressed the integration of video quality optimization and energy consumption.This study aims to create a more efficient method of encrypting video streaming in wireless sensor networks (WSNs) by combining selective Elliptic Curve Cryptography (ECC) with Evolution of Cub to Predator (ECP). The proposed method improves energy, distortion, and encryption performance by up to 10 dB more than other methods (EEP, UEP (GA), and MCS-RA) through the use of network resource allocation with ECP-RA in WSNs. Quantitative measures such as MSE, PSNR, RMSE, and SSIM demonstrate that this approach is superior to others.
A novel radial basis function description of a smooth implicit surface for musculoskeletal modelling
Authors: Cervenka Martin , Kohout Josef , Lipus Bogdan 
Published: April 2024

Abstract

As musculoskeletal illnesses continue to increase, useful computerised muscle modelling is crucial. This paper addresses this concern by proposing a mathematical model for a dynamic 3D geometrical surface representation of muscles using a Radial Basis Function (RBF) approximation technique. The objective is to obtain a smoother surface while minimising data use, contrasting it from classical polygonal (e.g., triangular) surface mesh models or volumetric (e.g., tetrahedral) mesh models. The novel method is tested on multiple data sets, and the experiments show good results according to the introduced metrics.
Affinity of Malassezia and other yeasts for pulmonary lipids
Authors: Ronda Violeta Esteban , Gilabert Pablo , Ferrer Consuelo , Gálvez Beatriz , Chiner Eusebi , Colom María Francisca 
Published: April 2024

Abstract

Pulmonary surfactant is the main substance that lines the epithelium of the human Lower Respiratory Tract (LRT). It has a high lipidic content, being dipalmitoyl-phosphatidylcholine (DPPC) the most abundant one. Although surfactants have a well-known antifungal activity for some yeast species, the important presence of species like Malassezia restricta in the lung mycobiome, leads to the idea that these yeasts may have some lipo-tolerance or even lipoaffinity for pulmonary lipids.
A culture-based study of affinity and tolerance to pulmonary lipids by yeasts described as important members of the lung microbiome has been carried out.
Ten species belonging to the genera Malassezia, Candida (including the new genera Nakaseomyces and Meyerozyma) and Cryptococcus, were tested for their ability to grow on media containing pulmonary lipids such as DPPC and commercial porcine surfactant. Lipoaffinity or lipo-tolerance was considered on the basis of their growth on standard media, considered as such the Modified Leeman Notman Agar (MLNA) for Malasseziaspecies, and the Sabouraud Dextrose Agar (SDA) for the other genera.
The addition of DPPC or surfactant to the media assayed resulted in increased growth of Malassezia yeasts and one of the Cryptococcus species. C. parapsilosis showed similar growth to the one obtained in the standard media and the other tested yeasts mainly showed lipo-tolerance but not lipoaffinity for these compounds.
To our knowledge, this is the first report on the influence of pulmonary lipids in the in vitro development of Malassezia spp and other yeasts members of the lung mycobiome.