bluuebunny/crossref_metadata_2025_split huggingface dataset:
O-Antigen decorations in Salmonella enterica play a key role in eliciting functional immune responses against heterologous serovars in animal models
Authors: Gasperini Gianmarco , Massai Luisa , De Simone Daniele , Raso Maria Michelina , Palmieri Elena , Alfini Renzo , Rossi Omar , Ravenscroft Neil , Kuttel Michelle M. , Micoli Francesca 
Published: February 2024

IntroductionDifferent serovars of Salmonella enterica cause systemic diseases in humans including enteric fever, caused by S. Typhi and S. Paratyphi A, and invasive nontyphoidal salmonellosis (iNTS), caused mainly by S. Typhimurium and S. Enteritidis. No vaccines are yet available against paratyphoid fever and iNTS but different strategies, based on the immunodominant O-Antigen component of the lipopolysaccharide, are currently being tested. The O-Antigens of S. enterica serovars share structural features including the backbone comprising mannose, rhamnose and galactose as well as further modifications such as O-acetylation and glucosylation. The importance of these O-Antigen decorations for the induced immunogenicity and cross-reactivity has been poorly characterized.MethodsThese immunological aspects were investigated in this study using Generalized Modules for Membrane Antigens (GMMA) as delivery systems for the different O-Antigen variants. This platform allowed the rapid generation and in vivo testing of defined and controlled polysaccharide structures through genetic manipulation of the O-Antigen biosynthetic genes.ResultsResults from mice and rabbit immunization experiments highlighted the important role played by secondary O-Antigen decorations in the induced immunogenicity. Moreover, molecular modeling of O-Antigen conformations corroborated the likelihood of cross-protection between S. enterica serovars.DiscussionSuch results, if confirmed in humans, could have a great impact on the design of a simplified vaccine composition able to maximize functional immune responses against clinically relevant Salmonella enterica serovars.
Comparison of diagnostic efficacy between 99mTc-methylene diphosphate SPECT/CT and MRI for bone and joint infections: a multicenter retrospective analysis
Authors: Gao Hao , Li Guoquan , Fu Congxiao , Ren Jun , Kang Fei , Luo Wen , Yin Qian , Zhou Cheng , Li Bo , Lu Shuaikun , Wang Hu , Zhang Yong , Zhang Yunfei 
Published: February 2024

ObjectiveThere is currently no non-invasive examination that can fully determine the diagnosis of osteomyelitis. SPECT/CT tomographic fusion imaging can provide both local metabolic activity and anatomical information to determine the condition and location. This study evaluates the diagnostic efficacy of 99mTc-MDP SPECT/CT in bone infections, compared to MRI.MethodsIn this multicenter retrospective study, 363 patients with suspected bone and joint infections or osteomyelitis were included. Participants underwent 99mTc-MDP SPECT/CT and/or MRI examinations, supplemented by pathogenic bacterial cultures and histopathological analysis.ResultsOnly SPECT/CT was tested in 169 patients, and only MRI was used in 116. 78 people have implemented both inspections and have detailed information. The diagnostic sensitivity and specificity of SPECT/CT for infection were 96% and 92% respectively, with an accuracy of 96%. For MRI, these figures were 88%, 84%, and 87% respectively.ConclusionThis represents the largest global study to date evaluating osteomyelitis and bone infection diagnosis using 99mTc-MDP SPECT/CT tomographic fusion imaging. The findings indicate that 99mTc-MDP SPECT/CT fusion imaging offers superior diagnostic accuracy compared to MRI. This is particularly evident in cases involving metallic implants and chronic infections. 99mTc-MDP SPECT/CT fusion imaging emerges as a highly suitable non-invasive diagnostic modality, facilitating enhanced clinical follow-up and treatment.
SKAP2 acts downstream of CD11b/CD18 and regulates neutrophil effector function
Authors: Bouti Panagiota , Klein Bart J. A. M. , Verkuijlen Paul J. H. , Schornagel Karin , van Alphen Floris P. J. , Taris Kees-Karel H. , van den Biggelaar Maartje , Hoogendijk Arie J. , van Bruggen Robin , Kuijpers Taco W. , Matlung Hanke L. 
Published: February 2024

BackgroundThe importance of CD11b/CD18 expression in neutrophil effector functions is well known. Beyond KINDLIN3 and TALIN1, which are involved in the induction of the high-affinity binding CD11b/CD18 conformation, the signaling pathways that orchestrate this response remain incompletely understood.MethodWe performed an unbiased screening method for protein selection by biotin identification (BioID) and investigated the KINDLIN3 interactome. We used liquid chromatography with tandem mass spectrometry as a powerful analytical tool. Generation of NB4 CD18, KINDLIN3, or SKAP2 knockout neutrophils was achieved using CRISPR-Cas9 technology, and the cells were examined for their effector function using flow cytometry, live cell imaging, microscopy, adhesion, or antibody-dependent cellular cytotoxicity (ADCC).ResultsAmong the 325 proteins significantly enriched, we identified Src kinase-associated phosphoprotein 2 (SKAP2), a protein involved in actin polymerization and integrin-mediated outside-in signaling. CD18 immunoprecipitation in primary or NB4 neutrophils demonstrated the presence of SKAP2 in the CD11b/CD18 complex at a steady state. Under this condition, adhesion to plastic, ICAM-1, or fibronectin was observed in the absence of SKAP2, which could be abrogated by blocking the actin rearrangements with latrunculin B. Upon stimulation of NB4 SKAP2-deficient neutrophils, adhesion to fibronectin was enhanced whereas CD18 clustering was strongly reduced. This response corresponded with significantly impaired CD11b/CD18-dependent NADPH oxidase activity, phagocytosis, and cytotoxicity against tumor cells.ConclusionOur results suggest that SKAP2 has a dual role. It may restrict CD11b/CD18-mediated adhesion only under resting conditions, but its major contribution lies in the regulation of dynamic CD11b/CD18-mediated actin rearrangements and clustering as required for cellular effector functions of human neutrophils.
A phase I trial of SON-1010, a tumor-targeted, interleukin-12-linked, albumin-binding cytokine, shows favorable pharmacokinetics, pharmacodynamics, and safety in healthy volunteers
Authors: Kenney Richard T. , Cini John K. , Dexter Susan , DaFonseca Manuel , Bingham Justus , Kuan Isabelle , Chawla Sant P. , Polasek Thomas M. , Lickliter Jason , Ryan Philip J. 
Published: February 2024

BackgroundThe benefits of recombinant interleukin-12 (rIL-12) as a multifunctional cytokine and potential immunotherapy for cancer have been sought for decades based on its efficacy in multiple mouse models. Unexpected toxicity in the first phase 2 study required careful attention to revised dosing strategies. Despite some signs of efficacy since then, most rIL-12 clinical trials have encountered hurdles such as short terminal elimination half-life (T½), limited tumor microenvironment targeting, and substantial systemic toxicity. We developed a strategy to extend the rIL-12 T½ that depends on binding albumin in vivo to target tumor tissue, using single-chain rIL-12 linked to a fully human albumin binding (FHAB) domain (SON-1010). After initiating a dose-escalation trial in patients with cancer (SB101), a randomized, double-blind, placebo-controlled, single-ascending dose (SAD) phase 1 trial in healthy volunteers (SB102) was conducted.MethodsSB102 (NCT05408572) focused on safety, tolerability, pharmacokinetic (PK), and pharmacodynamic (PD) endpoints. SON-1010 at 50-300 ng/kg or placebo administered subcutaneously on day 1 was studied at a ratio of 6:2, starting with two sentinels; participants were followed through day 29. Safety was reviewed after day 22, before enrolling the next cohort. A non-compartmental analysis of PK was performed and correlations with the PD results were explored, along with a comparison of the SON-1010 PK profile in SB101.ResultsParticipants receiving SON-1010 at 100 ng/kg or higher tolerated the injection but generally experienced more treatment-emergent adverse effects (TEAEs) than those receiving the lowest dose. All TEAEs were transient and no other dose relationship was noted. As expected with rIL-12, initial decreases in neutrophils and lymphocytes returned to baseline by days 9-11. PK analysis showed two-compartment elimination in SB102 with mean T½ of 104 h, compared with one-compartment elimination in SB101, which correlated with prolonged but controlled and dose-related increases in interferon-gamma (IFNγ). There was no evidence of cytokine release syndrome based on minimal participant symptoms and responses observed with other cytokines.ConclusionSON-1010, a novel presentation for rIL-12, was safe and well-tolerated in healthy volunteers up to 300 ng/kg. Its extended half-life leads to a prolonged but controlled IFNγ response, which may be important for tumor control in patients.Clinical trial registrationhttps://clinicaltrials.gov/study/NCT05408572, identifier NCT05408572.
Vestibular function in cases of posterior semicircular canal canalolithiasis and cupulolithiasis
Authors: Wenyan Xu , Lifeng Yue , Jing Wu , Hui Jiang 
Published: February 2024

ObjectiveTo analyze and compare the vestibular function of posterior canal cupulolithiasis and canalolithiasis.MethodsThe results of posterior cupulolithiasis in 45 cases, posterior canalolithiasis in 122 cases and 19 healthy controls were analyzed retrospectively.ResultsThe abnormal rates of vHIT in the canalolithiasis group and the cupulolithiasis group were 42.6 and 37.8%, respectively, both higher than those in the control group (both p &lt; 0.05); there was no statistically significant difference between two BPPV groups (p = 0.573). The abnormal vHIT in 76.9% of the canalolithiasis cases and 82.4% of the cupulolithiasis cases showed normal gain with saccades, with no difference between the groups (p = 0.859). The lesion location of vHIT in the two groups did not show a correlation with the affected side of BPPV (both p &gt; 0.05). 84.4% of canalolithiasis and 65.0% of cupulolithiasis had abnormal VEMP results, with no significant differences in abnormality rates or sides (both p &gt; 0.05). Abnormal results of VEMPs did not show any correlation with side (p &gt; 0.05). The results of pc-ca and pc-cu were both abnormal in 14 cases and 7 cases, and there was no correlation between the site and side of the injury (all p &gt; 0.05).ConclusionThe results of vHIT and VEMP in pc-cu and pc-ca were partially abnormal, but they did not show any correlation with side of BPPV. It can be considered that there are scattered vestibular peripheral organ damage in both groups.
Linear association of compound dietary antioxidant index with hyperlipidemia: a cross-sectional study
Authors: Zhou Haoran , Li Tianshu , Li Jie , Zheng Dongdong , Yang Jie , Zhuang Xin 
Published: February 2024

BackgroundThere is growing evidence that antioxidant-rich diets may prevent hyperlipidemia. However, the relationship between the Composite Dietary Antioxidant Index (CDAI) and hyperlipidemia is unclear. The CDAI is a composite score reflecting the antioxidant content of an individual’s diet, and this study aimed to investigate the relationship between CDAI and hyperlipidemia.MethodsThe study used the 2003–2018 National Health and Nutrition Examination Survey (NHANES) database for cross-sectional analyses and included 27,626 participants aged 20 years and older. The CDAI, which includes vitamins A, C, and E, zinc, selenium, and carotenoids, was calculated based on dietary intake reported in a 24-h recall interview. Hyperlipidemia was defined by the National Cholesterol Education Program (NCEP). Covariates included age, sex, race, education, marriage, household poverty-to-income ratio (PIR), glomerular filtration rate (eGFR), body mass index (BMI), energy, carbohydrates, total fat, cholesterol, smoking, alcohol consumption, hypertension, diabetes mellitus, coronary heart disease, and lipid-lowering medications. The association between CDAI and hyperlipidemia was explored through multiple logistic regression analyses and smoothed curve fitting. We also performed subgroup analyses and interaction tests to verify the relationship’s stability.ResultsAfter adjusting for potential confounders, CDAI was negatively associated with the risk of developing hyperlipidemia (OR 0.98, 95% CI 0.96–0.99, p &lt; 0.01). The results of weighted regression models stratified by quartiles of CDAI (−8.664 ≤ Q1 ≤ −2.209, −2.209 &lt; Q2 ≤ −0.002, −0.002 &lt; Q3 ≤ 2.774, 2.774 &lt; Q4 ≤ 124.284), fully adjusted for confounding variables, indicated that compared with the bottom quartile (Q1) of the CDAI, Q2, Q3, and Q4 of participants had a lower advantage ratio (Q2: OR 0.91, 95% CI 0.78–1.06, p &lt; 0.21; Q3: OR 0.85, 95% CI 0.73–1.00, p &lt; 0.05; and Q4: OR 0.77, 95% CI 0.64–0.94, p &lt; 0.01), which was confirmed by a test for trend (p &lt; 0.05). Smoothed curve fit analysis showed linearity (p for non-linear = 0.0912). In summary, there is a linear negative relationship between CDAI and the risk of developing hyperlipidemia. Subgroup analyses by age, sex, ethnicity, education level, marriage, tobacco status, alcoholic drinking, body mass index (BMI), hypertension, and diabetes did not indicate strong interactions.ConclusionIn this large cross-sectional study, there was a linear negative association between CDAI and hyperlipidemia among US adults. Therefore increase antioxidant rich foods in your life as a prevention of hyperlipidemia.
An evaluation of Astragali Radix with different growth patterns and years, based on a new multidimensional comparison method
Authors: Wang Yapeng , Yuan Changsheng , Zhao Jiachen , Liu Yunxiang , Tian Chunfang , Qian Jinxiu , Nan Tiegui , Kang Liping , Liu Yanmeng , Zhan Zhilai , Huang Luqi 
Published: February 2024

IntroductionWith the depletion of wild Astragali Radix (WA) resources, imitated-wild Astragali Radix (IWA) and cultivated Astragali Radix (CA) have become the main products of Astragali Radix. However, the quality differences of three growth patterns (WA, IWA, CA) and different growth years of Astragali Radix have not been fully characterized, leading to a lack of necessary scientific evidence for their use as substitutes for WA.MethodsWe innovatively proposed a multidimensional evaluation method that encompassed traits, microstructure, cell wall components, saccharides, and pharmacodynamic compounds, to comprehensively explain the quality variances among different growth patterns and years of Astragali Radix.Results and discussionOur study showed that the quality of IWA and WA was comparatively similar, including evaluation indicators such as apparent color, sectional structure and odor, thickness of phellem, diameter and number of vessels, morphology of phloem and xylem, and the levels and ratios of cellulose, hemicellulose, lignin, sucrose, starch, water-soluble polysaccharides, total-saponins. However, the content of sucrose, starch and sorbose in CA was significantly higher than WA, and the diameter and number of vessels, total-flavonoids content were lower than WA, indicating significant quality differences between CA and WA. Hence, we suggest that IWA should be used as a substitute for WA instead of CA. As for the planting years of IWA, our results indicated that IWA aged 1-32 years could be divided into three stages according to their quality change: rapid growth period (1-5 years), stable growth period (6-20 years), and elderly growth period (25-32 years). Among these, 6-20 years old IWA exhibited consistent multidimensional comparative results, showcasing elevated levels of key active components such as water-soluble polysaccharides, flavonoids, and saponins. Considering both the quality and cultivation expenses of IWA, we recommend a cultivation duration of 6-8 years for growers. In conclusion, we established a novel multidimensional evaluation method to systematically characterize the quality of Astragali Radix, and provided a new scientific perspective for the artificial cultivation and quality assurance of Astragali Radix.
Associations between internet addiction and school engagement among Turkish college students: mediating role of psychological distress
Authors: Öztekin Gülçin Güler 
Published: February 2024

IntroductionInternet addiction has become a subject of growing concern with adverse consequences. This study aimed to investigate the mediating effect of psychological distress in the relationship between internet addiction and school engagement.MethodsData were obtained from 732 Turkish college students (M = 20.98, SD = 2.58). The data were collected through Young’s Internet Addiction Test, Depression, Stress and Anxiety Scale (DASS21)-Short Form, and University Student Engagement Inventory. Mediation analysis was performed to investigate the mediating effects of depression, anxiety, and stress in the association between internet addiction and school engagement.ResultsThe results of the study showed that internet addiction was positively associated with psychological distress and negatively associated with school engagement, whereas psychological distress had a negative relationship with school engagement. This study also found that depression, anxiety, and stress acted as mediators in the relationship between internet addiction and behavioral engagement, emotional engagement, and cognitive engagement.ConclusionThis study has provided evidence that young adults with tendency of internet addiction may experience school engagement problems as well as psychological problems. Our findings underscore the need for special educational intervention programs aimed at encouraging college youth to limit their internet use for their future due to its negative consequences and raising awareness in this vulnerable group and their families.
Direction of arrival estimation using the rotating equatorial microphone
Authors: Lawrence Jeremy , Ahrens Jens , Peters Nils 
Published: February 2024

Introduction: Direction of arrival (DOA) estimation of sound sources is an essential task of sound field analysis which typically requires two or more microphones. In this study, we present an algorithm that allows for DOA estimation using the previously designed Rotating Equatorial Microphone prototype, which is a single microphone that moves rapidly along a circular trajectory, introducing DOA-dependent periodic distortions in the captured signal.Methods: Our algorithm compensates for the induced spectral distortions caused by the REM’s circular motion for multiple DOA candidates. Subsequently, the best DOA candidate is identified using two distortion metrics. We verify our approach through numerical simulations and practical experiments conducted in a low-reverberant environment.Results: The proposed approach localizes unknown single-frequency sources with a mean absolute error of 23 degrees and unknown wideband sources with a mean absolute error of 5.4 degrees in practice. Two sources are also localizable provided they are sufficiently separated in space.Conclusion: Whilst previous work only allowed for DOA estimation of a single monochromatic sound source with a known frequency, our DOA estimation algorithm enables localization of unknown and arbitrary sources with a single moving microphone.
Sociocultural predictors of immigrant adjustment and well-being
Authors: Shekriladze Ia , Javakhishvili Nino 
Published: February 2024

IntroductionResearch shows that culture change may pose risks to immigrant wellbeing. Our study examined adult Georgians (N = 431) residing in Greece, Italy, and Germany, and explored associations between their demographic characteristics, sociocultural adjustment, and psychological well-being outcomes.MethodsConducted via electronic self-report survey, the cross-sectional study measured participants’ levels of sociocultural adjustment, psychological adjustment, and depression along with the willingness to interact with host nationals, perceived sense of discrimination, history of being undocumented, age and length of relocation, and fluency in host language. The study also examined differences in three subsamples from the standpoint of intercultural distance. Sociocultural Adjustment Scale, Brief Psychological Adaptation Scale, Center for Epidemiologic Studies Depression Scale and Host Interaction Scale were used to measure the corresponding variables. Perceived history of discrimination was measured by a Likert-scale question about discrimination in a host country. Intercultural distance was established by Hofstede cultural compass and was estimated to be the smallest with Greece and the largest with Germany.ResultsDepression was positively predicted by histories of discrimination and illegal immigration, host language fluency upon relocation, and was negatively predicted by sociocultural adjustment. Psychological adjustment was positively predicted by sociocultural adjustment, willingness to interact with host nationals, and ongoing language fluency, while perceived sense of discrimination, age, and poor financial state acted as negative predictors. Finally, sociocultural adjustment acted as the strongest determinant of wellbeing predicting both lower depression and higher psychological adjustment.DiscussionOur findings suggested that adjustment in diverse sociocultural domains was the most critical for the immigrants’ psychological well-being along with the lack of perceived discrimination. Additional factors associated with the better adaptation outcomes included younger age, willingness to interact with host nationals, language fluency, better financial standing and no history of being undocumented. The results also indicated that host language proficiency upon relocation may contribute to migrant susceptibility, whereas intercultural distance may be overshadowed in importance by acculturation conditions. The findings illustrate the complexity of migration and culture change and point to the superiority of wholistic policies and practices in promoting smooth transition of immigrant populations.
Impact of menstrual cycle or combined oral contraception on elite female cyclists' training responses through a clustering analysis of training sessions
Authors: Carlin Hugo , Dupuit Marine , Storme Florent , Chassard Tom , Meignié Alice , Sachet Iris , Brunet Emanuel , Toussaint Jean-François , Antero Juliana 
Published: February 2024

Objectives(i) To classify training sessions of elite female cyclists according to an intensity index based on a longitudinal follow-up using multiparametric data collected in situ (ii) to measure the effect of estimated menstrual cycle (MC) phases and oral contraceptive pills (OC) phases on the athletes' training responses on each type of training identified.MethodThirteen elite French cyclists were followed up over 30 months and 5,190 training sessions were collected and 81 MC/OCs full cycles analyzed. Power sensors and position devices captured training data in situ, which was summarized into 14 external load variables. Principal Component Analysis and K-means clustering were used to identify cycling sessions according to an intensity load index. The clusters were then verified and categorized through the analysis of heart rate and rate of perceived effort. A calendar method was used to estimate 3 phases of the MC: menstruation, mid-cycle phase (MP) and late-cycle phase (LP). Two phases were defined among monophasic OC users: pills' taking/withdrawal.ResultsFour main types of training effort were identified: Intensive, Long, Medium and Light. In the MC group (n = 7; 52 cycles), the intensity index is 8% higher during the mid-cycle (vs. menstrual phase, p = 0.032) in the Intensive effort sessions. No differences were observed in Long, Medium or Light effort, nor between the phases of pills' taking/withdrawal among OC users.ConclusionThe clustering analyses developed allows a training classification and a robust method to investigate the influence of the MC/OC in situ. A better training response during the mid-cycle when the sessions are the most intense suggest an impact of the MC when the athletes approach their maximal capacity.