bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal quality of life data can provide insights on the impact of adjuvant treatment for pancreatic cancer—Subset analysis of the ESPAC‐1 data
Authors: Carter Ross , Stocken Deborah D. , Ghaneh Payla , Bramhall Simon R. , Olah Attila , Kelemen Deszo , Bassi Claudio , Friess Helmut , Dervenis Christo , Spry Nigel , Büchler Markus W. , Neoptolemos John P. 
Published: January 2009

AbstractThe European Study Group for Pancreatic Cancer (ESPAC‐1) study is the largest study of adjuvant treatment for pancreatic ductal adenocarcinoma to date and confirmed a survival advantage for adjuvant chemotherapy but not for chemoradiation. The importance of parallel evaluation of survival and quality of life (QoL) has been recognized as fundamental and the aim was to assess QoL and quality adjusted survival. A longitudinal QoL study on a subset of ESPAC‐1 patients who prospectively completed the EORTC QLQ C‐30 questionnaire during treatment and follow‐up. An integrated quality‐survival product method was used to adjust any treatment effect on survival by a function of measured QoL, calculated over a restricted 24‐month‐period (QALM‐24). Three hundred and sixteen patients completed 1,201 questionnaires. There were no differences between treatment groups in dimension scores at baseline (randomization). For the chemotherapy group, the mean Quality Adjusted Life Months over 24 months (QALM‐24) was 9.6 (95% CI: 8.7, 11.2) months compared with the mean QALM‐24 of 8.6 (95% CI: 7.6, 10.5) months for the no chemotherapy group. For the chemoradiation group, the mean QALM‐24 was 7.1 (95% CI: 6.0, 9.0) months compared with the mean QALM‐24 of 8.1 (95% CI: 7.0, 10.0) months for the no chemoradiation group. The previously reported survival advantage supporting the use of adjuvant chemotherapy is maintained when adjusted using quality adjusted survival methodology. Chemotherapy provided on average an additional 1.0 quality‐adjusted life months within a restricted 2‐year time period from the time of resection. © 2009 UICC
Mental health in infants with esophageal atresia
Authors: Faugli Anne , Emblem Ragnhild , Bjørnland Kristin , Diseth Trond H. 
Published: January 2009

AbstractChronic somatic illness in infancy may challenge the development of mental health and impinge the infant's capability to form close interpersonal relationships. Esophageal atresia (EA) is a congenital anomaly requiring neonatal surgery, medical aftertreatment, and extended hospitalization. The aim of the study was to assess mental health and to find prognostic factors for mental health among infants with EA. Thirty‐nine infants treated consecutively during 2000 to 2003 and their mothers were included. Infant mental health was assessed by Diagnostic Classification: 0–3 (Zero to Three, 1994). Medical and environmental data were collected from medical records and semistructured interview with the mothers. Child development was assessed with the Bayley scales, second edition (N. Bayley, 1993). Maternal psychological distress, anxiety, and child temperament were assessed by self‐report questionnaires: the General Health Questionnaire, 30‐item version (D. Goldberg & P. Williams, 1988); the State Trait Anxiety Inventory (C.D. Spielberger, R. Gorsuch, & R. Lushene, 1970); and the Infant Behaviour Questionnaire (M.K. Rothbart, 1981). Thirty‐one percent of the infants with EA showed mental health disorders by 1 year of age. Prognostic factors predicting mental health were posttraumatic symptoms reported by mother, more than one operation, mechanical ventilation beyond 1 day, and moderate/severe chronic family strain. Relational trauma, vulnerable attachment, and impaired self‐development are highlighted as possible pathways for psychopathology. Children with EA are vulnerable to mental health disorders, and this study may help clinicians to identify children at risk.
Infant irritability: The impact of fetal alcohol exposure, maternal depressive symptoms, and low emotional support from the husband
Authors: Lemola Sakari , Stadlmayr Werner , Grob Alexander 
Published: January 2009

AbstractObjectives: The aim of this study was to investigate the impact of fetal alcohol exposure, maternal depressive symptoms, and low emotional support from the husband on infant irritability in the first one and a half years of life. Four models describing the interplay of these factors were assessed: A direct effect model, an interaction or threshold model, a mediational model and a transactional model.Method: A sample of initially 458 women was assessed in a prospective 3‐wave study across the first 17 months after childbirth. Fetal alcohol exposure was questioned retrospectively six weeks after birth. Infant irritability was reported by the mothers and fathers.Results: Support for the direct effect model and the interaction model could be found: Fetal alcohol exposure and low emotional support from the husband were associated with increased infant irritability at 5 months. The impact of fetal alcohol exposure was moderated by postnatal depressive symptoms. More irritability was reported if both risk factors, prenatal alcohol exposure and maternal depressive symptoms, were present. Infant irritability and maternal depressive symptoms were associated cross‐sectionally. At the age of 17 months only a main effect of prenatal alcohol exposure on irritability could be found.Conclusion: Direct effects of fetal alcohol exposure, maternal depressive symptoms, and low emotional support from the husband on infant irritability as well as an interaction between fetal alcohol exposure and maternal depressive symptoms were revealed. The interaction can be understood in terms of a diathesis‐stress model. However, no longitudinal associations between maternal depressive symptoms and infant irritability could be found.
Promoting infant well‐being in the context of maternal depression by supporting the father
Authors: Fletcher Richard 
Published: January 2009

AbstractMaternal depression is recognized as posing a significant risk to the healthy development of infants. Guided by attachment theory, interventions have focused on the distressed mother's relationship with her infant. While interventions which include fathers are reported in the literature, the processes involved in the fathers' support of a distressed mother and in his interactions with his infant in the context of maternal depression remain unexplored. In the case study described here, home‐visiting support was directed to the father in a couple in which the mother had been treated for depression after the birth. The observed effects of the intervention on father–infant interaction and the “knock‐on” effects on the mothers' parenting suggest that targeting support to fathers in distressed families should be considered. Points for a service protocol are provided to incorporate fathers into the identification and treatment of maternal distress.
Quantitative proteomic analysis of differential proteins in the stroma of nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissue
Authors: Li Mei‐Xiang , Xiao Zhi‐Qiang , Liu Ying‐Fu , Chen Yong‐Heng , Li Cui , Zhang Peng‐Fei , Li Mao‐Yu , Li Feng , Peng Fang , Duan Chao‐Jun , Yi Hong , Yao Hui‐Xin , Chen Zhu‐Chu 
Published: January 2009

AbstractThe importance of stromal cells and the factors that they expressed during cancer initiation and progression have been highlighted by recent literature. To identify the stromal proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, we assessed differences in protein expression of the stroma from NPC and normal nasopharyngeal epithelium tissues (NNET) using a quantitative proteomic approach combined with laser capture microdissection (LCM). LCM was performed to purify stromal cells from the NPC and NNET, respectively. The differential proteins between the pooled microdissected tumor and normal stroma were analyzed by two‐dimensional difference gel electrophoresis (2D‐DIGE) combined with mass spectrometry (MS). Twenty differential proteins were identified, and the expression and location of two differential proteins (L‐plastin and S100A9) were further confirmed by Western blotting and immunohistochemical analysis. Our results will be helpful to study the role of stroma in the NPC carcinogenesis, as well as discover the interaction between NPC cells and their surrounding microenvironment. J. Cell. Biochem. 106: 570–579, 2009. © 2009 Wiley‐Liss, Inc.
Transcriptional regulation of fibrillin‐2 gene by E2F family members in chondrocyte differentiation
Authors: Yanagino Takuya , Yuasa Keizo , Nagahama Masami , Matsuda Yoshiko , Tsuji Akihiko 
Published: January 2009

AbstractMutation in fibrillin‐2, a major structural component of extracellular microfibrils in connective tissue, results in the autosomal dominant disease congenital contractural arachnodactyly. This genetic disease is characterized by dolichostenomelia and arachnodactyly, in addition to contractures of the large joints and abnormal pinnae formation, thus indicating the significance of fibrillin‐2 in chondrogenesis. In this study, we investigated the transcriptional regulation of fibrillin‐2 in chondrogenic differentiation. Although mRNA expression of fibrillin‐1, a highly homologous protein to fibrillin‐2, remained almost unchanged during chondrogenesis of mouse ATDC5 cells, fibrillin‐2 mRNA expression varied. Fibrillin‐2 was highly expressed at the early stage and declined progressively during differentiation. The 5′‐flanking region of the fibrillin‐2 gene contains potential binding sites for E2F, Runx, AP‐2, and Sox transcription factors. The promoter activity of fibrillin‐2 decreased markedly following deletion and mutagenesis of the E2F binding site between −143 and −136 bp. Overexpression of E2F1 resulted in a marked increase in its promoter activity, whereas expression of other transcription factors including AP‐2α and Runx2 had no effect. The increase in promoter activity by E2F1 was completely suppressed by the coexpression of E2F4. E2F2 and E2F3 had positive effects on the promoter activity. Although ATDC5 cells expressed transcripts for the E2F family genes at all stages of differentiation, the expression profiles differed. E2F1 expression remained almost unchanged, whereas E2F4 expression increased markedly at the late stage of differentiation. These results indicated that coordinated expression of the E2F family is critical for the transcriptional regulation of fibrillin‐2 during chondrogenesis. J. Cell. Biochem. 106: 580–588, 2009. © 2009 Wiley‐Liss, Inc.
Anti‐C1q antibodies: association with nephritis and disease activity in systemic lupus erythematosus
Authors: Moura Carlos Geraldo , Lima Isabella , Barbosa Lúcio , Athanazio Daniel , Reis Eliana , Reis Mitermayer , Burlingame Rufus W. , Santiago Mittermayer B. 
Published: January 2009

AbstractBackground: Anti‐C1q antibodies have been described in systemic lupus erythematosus (SLE) as well as in other connective tissue diseases. They have been considered as a marker for disease activity and presence of nephritis.Objective: The aim of this study was to determine the prevalence of anti‐C1q antibodies in Brazilian lupus patients as well as analyze their association with different clinical and serologic parameters.Methods: Sera from 81 SLE patients, based on the American College of Rheumatology (ACR) criteria, were collected from a lupus referral outpatient clinic in Salvador, Brazil. Antibodies to C1q were detected by an enzyme‐linked immunoassay (ELISA) kit and antibodies to other cellular antigens identified by indirect immunofluorescence on HEp‐2 cell substrate (ANA), or Crithidia luciliae (dsDNA), and to nucleosome by ELISA. A cutoff of 20 U wasestablished for anti‐C1q and antinucleosome assays.Results: Anti‐C1q antibodies were detected in 39.5% (32/81) of SLE sera. The presence of anti‐C1q antibodies was associated with proteinuria (P=0.028) but not with other laboratory or clinical features, such as antinucleosome or anti‐dsDNA antibodies, hematuria, urinary casts or renal failure, leukopenia, pericarditis, pleuritis, malar rash, seizures, and psychosis. There was a positive correlation between the titers of anti‐C1q antibodies and the systemic lupuis erythematosus disease activity index (SLEDAI) score (r=0.370; P=0.001).Conclusion: This study in Brazilian SLE patients confirms previous findings of the association of anti‐C1q antibodies with nephritis and disease activity. J. Clin. Lab. Anal. 23:19–23, 2009. © 2009 Wiley‐Liss, Inc.
Silent myocardial ischemia is associated with altered plasma phospholipids
Authors: Lin Hailong , Zhang Jing , Gao Peng 
Published: January 2009

AbstractTimely and accurate confirmation of the occurrence of silent myocardial ischemia (SMI) is critical both for prevention and therapy management. Metabolomics assay may offer an alternative for SMI differentiation and altered biomolecule discovery in addition to traditional measures. In this study, plasma samples were obtained from 14 diagnosed SMI subjects and 25 healthy controls and analyzed by liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry in view of metabolomics. Obtained data were subjected to orthogonal signal correction partialleast‐squares discriminate analysis. Multi‐variate statistic analysis indicated a clear separation between the two studied groups. Plasma concentration fluctuation of four kinds of phospholipids showed tight relationship with the occurrence of SMI, among which 1‐linoleoylglycerophosphocholine (C18:2) was decreased statistically in SMI population (P=0.01). The plasma phospholipids' changes were before enzymatic alteration in SMI, which might be a useful complementary reference to facilitate SMI diagnosis. J. Clin. Lab. Anal. 23:45–50, 2009. © 2009 Wiley‐Liss, Inc.
Evaluation of memory immune response to mycobacterium extract among household contact of tuberculosis cases
Authors: Cavalcanti Y.V.N. , Pereira V.R.A. , Reis L.C. , Ramos A.L.G. , Luna C.F. , Nascimento E.J.M. , Lucena‐Silva N. 
Published: January 2009

AbstractThe human immune response to tuberculosis (TB) is especially mediated by T CD4+lymphocytes. However, more studies are needed in order to understand the exact role of each cytokine in the mechanisms for cures. In this article, our aim was to analyze the production of TNF‐α, IL‐10, and IFN‐γ in peripheral blood mononuclear cells (PBMCs) among the household contacts of common primary TB cases, with or without histories of active TB infection, who were negative to parasitological and HIV tests. In order to characterize the cytokine production, PBMCs from these groups werestimulated with whole‐protein extract of M. tuberculosis (WPE) antigen (rAgTb) for 24 and 48 hr. The culture supernatants were collected and IFN‐γ, TNF‐α, and IL‐10 were assayed using capture ELISA. There were no statistical differences between primary TB cases and their household contacts with or without previous histories of lung TB. Our results suggest that T memory cells, T regulatory cells, and the Th1/Th2 dichotomy may be responsible for the results described in this article. Further studies are currently underway. J. Clin. Lab. Anal. 23:57–62, 2009. © 2009 Wiley‐Liss, Inc.
De‐sulfation of MG‐63 cell glycosaminoglycans delays in vitro osteogenesis, up‐regulates cholesterol synthesis and disrupts cell cycle and the actin cytoskeleton
Authors: Kumarasuriyar A. , Lee I. , Nurcombe V. , Cool S.M. 
Published: January 2009

AbstractGlycosaminoglycan (GAG) sugars are largely responsible for the bioactivity of the proteoglycan proteins they decorate, and are particularly important for mediating the processes of cell attachment and growth factor signaling. Here, we show that chlorate‐induced de‐sulfation of GAGs expressed by MG‐63 osteosarcoma cells results in delayed cell proliferation when the cells are exposed to chlorate for short or medium periods, but a disrupted mineralization without altered cell proliferation in response to long‐term chlorate exposure. Analysis of GAG‐binding growth factor activity indicated that chlorate disrupted BMP2/noggin signaling, but not FGF2 activity. Microarray analyses, which were confirmed by subsequent cell‐based assays, indicated that chlorate predominantly disrupted the cell cycle and actin cytoskeleton and upregulated cholesterol synthesis, without affecting cell migration or attachment. Furthermore, we observed that disruption of the functions of the proteoglycan syndecan‐4 replicated phenotypes induced by chlorate, implicating a primary role for this proteoglycan in providing bioactivity for these cells. J. Cell. Physiol. 219: 572–583, 2009. © 2009 Wiley‐Liss, Inc.
Chaperonin 10 as an endothelial‐derived differentiation factor: Role of glycogen synthase kinase‐3
Authors: Dobocan Monica C. , Sadvakassova Gulzhakhan , Congote Luis F. 
Published: January 2009

AbstractThe anti‐inflammatory peptide early pregnancy factor/chaperonin 10 (cpn10) was identified by 2D‐electrophoresis/mass spectrometry as one of the proteins increased in human umbilical cord endothelial cells (HUVEC) after treatment with erythropoietin (EPO). EPO increased the amount of cpn10 released into the medium of HUVEC cultures, despite the absence of a secretory signal peptide. Although immunosupressive agents would represent an indirect advantage for red cell formation under conditions of infection and inflammation, it is possible that cpn10 may have direct effects on erythroid cells. We show that the chaperonin decreased cell proliferation in cultures of the erythroleukemia cell line K562 and increased the amounts of the erythroid differentiation markers glycophorin A and hemoglobin in TF‐1 cells. Nevertheless, cpn10 is not a specific erythroid cell differentiation factor, because monolayers of skin fibroblasts overexpressing cpn10 had significantly higher levels of the differentiation marker collagen I than normal fibroblasts. Nothing is known about the mechanism of action of cpn10 in its capacity as a general differentiation factor. An analysis of early changes taking place in K562 cells after incubation with cpn10 using antibody microarrays identified several phosphorylation events, including a decrease of GSK‐3α phosphorylation. Further studies in TF‐1 cells indicated that cpn10 decreased the phosphorylation of cofilin‐1 while stimulating that of GSK‐3β. Furthermore, glycophorin A production decreased in the presence of a GSK‐3 inhibitor in the same cells. These experiments support the idea that GSK‐3‐regulated signal transduction pathways are not only important for stem cell maintenance but may be involved in events controlling cell differentiation. J. Cell. Physiol. 219: 470–476, 2009. © 2009 Wiley‐Liss, Inc.
Evaluation of applied cathode potential to enhance biocathode in microbial fuel cells
Authors: Liang Peng , Fan Mingzhi , Cao Xiaoxin , Huang Xia 
Published: January 2009

AbstractBACKGROUND: The biocathode is proving to be a promising feature for development of the microbial fuel cell (MFC), although much work remains to be done to increase its power generation. This study aimed to enhance the performance of a biocathode by applying selected cathode potential.RESULTS: When five two‐chambered MFCs were operated at selected cathode potentials of 142, 242, 342, 442, or 542 mV (vs standard hydrogen electrode), those MFCs with selected potentials lower than 342 mV could start up, and the highest power density of 0.11 W m−3 was obtained at a selected potential of 242 mV. An inner‐biocathode MFC was then constructed and operated at a start‐up cathode potential of 242 mV for 30 days. The open circuit cathode potential increased from 477 ± 9 mV to 572 ± 8 mV compared with the potential of the initially abiotic cathode, resulting in an increase in the maximum power density (4.25 ± 0.16 W m−3) of 106%. In addition, tests of continuous operation showed that a loading rate of 135 mg COD L−1 d−1 was optimal for obtaining maximum power generation in the system developed for this study.CONCLUSION: The results indicated that an optimal cathode potential of 242 mV enhanced the performance of a biocathode using oxygen as the electron acceptor. Copyright © 2009 Society of Chemical Industry
High power generation with simultaneous COD removal using a circulating column microbial fuel cell
Authors: Kargi Fikret , Eker Serkan 
Published: January 2009

AbstractBACKGROUND: A circulating column microbial fuel cell (MFC) with Cu anode and AuCu air cathode was used for power generation and chemical oxygen demand (COD) removal from synthetic wastewater. The column was operated in repeated‐fed batch mode using acclimated anaerobic sludge. The contents of the column MFC were circulated while the feed wastewater was fed to the reactor in fed‐batch mode. Effects of feed COD concentration and COD loading rate on voltage difference, power density and percentage COD removal were investigated.RESULTS: The highest voltage difference (650 mV), power density (40 W m−2) were obtained with a feed COD of 6400 mg L−1, yielding 45% COD removal with a COD loading rate of nearly 90 mg h−1. Low COD loadings (<90 mg h−1) caused substrate limitations, and high loadings (>90 mg h−1) resulted in inhibition of COD removal and power generation. The highest percentage COD removal (50%) was obtained with feed COD content of 10.35 g L−1 or a COD loading rate of 145 mg h−1.CONCLUSION: The power densities obtained with the circulating column MFC were considerably higher than those reported in the literature due to elimination of mass transfer limitations by the high circulation rates, proximity of electrodes and small anode surface area used in this study. Further improvements may be possible with optimization of the operating parameters. Copyright © 2009 Society of Chemical Industry
Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry
Authors: Li Hua‐Yu , Wang Shao‐Min , Liu Hong‐Min , Bu Shan‐Shan , Li Juan , Han Dong , Zhang Ming‐zhi , Wu Guang‐Yin 
Published: January 2009

AbstractUrinary‐modified nucleosides have a potential role as cancer biomarkers for a number of malignant diseases. High performance liquid chromatography (HPLC) was combined with full‐scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify purine nucleosides purified from urine. Potential purine nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner, numerous modified purine nucleosides were identified in the urine samples from cancer patients including xanthine, adenosine, N1‐methyladenosine, 5′‐deoxy‐5′‐methylthioadenosine, 2‐methyladenosine, N6‐threonylcarbamoyladenosine, inosine, N1‐methylinosine, guanosine, N1‐methylguanosine, N7‐methylguanine, N2‐methylguanosine, N2,N2‐dimethyguanosine, N2,N2,N7‐trimethylguanosine. Furthermore, a number of novel purine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data including N3‐methyladenosine, N7‐methyladenine, 5′‐dehydro‐2′‐deoxyinosine, N3‐methylguanine, O6‐methylguanosine, N1,N2,N7‐trimethylguanosine, N1‐methyl‐N2‐ethylguanosine and N7‐methyl‐N1‐ethylguanosine. Copyright © 2009 John Wiley & Sons, Ltd.