bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of Tomato leaf curl New Delhi virus Infecting Tomato in Bangladesh
Authors: Maruthi M. N. , Rekha A. R. , Cork A. , Colvin J. , Alam S. N. , Kader K. A. 
Published: May 2007

Tomato is an important cash crop for resource-poor farmers and accounts for 20% of the 2 million t of vegetables grown annually in Bangladesh. Tomato cultivation is affected by Tomato leaf curl virus (ToLCV), which can cause as much as 100% yield loss. Plants exhibiting typical ToLCV disease symptoms of yellowing, severe leaf curling, and stunting were collected at Jessore, Bangladesh during September 2003. The putative virus was transmitted from tomato to tomato by the whitefly Bemisia tabaci. In two separate experiments, 100% transmission was achieved by using 10 viruliferous B. tabaci adults for each of the 20 test plants that was confirmed by comparing the symptoms on test and virus source plants. Total DNAs were extracted from the symptomatic leaves, and the putative viral genomes were amplified by polymerase chain reaction by using the Deng A and B primers (1). Sequences generated from these primers were used to design virus-specific primers that were used to obtain complete viral sequences. Full-length DNA-A (2,740 nt; GenBank Accession No. AJ875157) and DNA-B (2,688 nt; GenBank Accession No. AJ875158) sequences of a bipartite Tomato leaf curl New Delhi virus from Jessore (ToLCNDV-[Jes]) were obtained, which were most similar to the corresponding sequences of ToLCNDV-(Lucknow) (GenBank Accession No. Y16421) at 95.7% and Tomato leaf curl Gujarat virus-(Varanasi) (Gen-Bank Accession No. AY190291) at 90.6% nt identities, respectively. DNA-A sequences had only 73.2% nt identity with the previously reported monopartite Tomato leaf curl Bangladesh virus (GenBank Accession No. AF188481) (2), confirming the occurrence of mono- and bipartite bego-moviruses in Bangladesh. The virus diversity poses a challenge for ToLCVD management in Bangladesh. References: (1) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (2) S. K. Green et al. Plant Dis. 85:1286, 2001.
Phytophthora Leaf Spot and Foliar Blight of Pieris japonica Caused by Phytophthora citricola in California
Authors: Koike S. T. , Browne G. T. , Bhat R. G. , Lee R. C. M. , Tjosvold S. A. , Buermeyer K. 
Published: May 2007

During 2004, containerized nursery stock of lily-of-the-valley-bush (Pieris japonica ‘Flamingo’, family Ericaceae) in Santa Cruz County was affected by a foliar disease. Symptoms consisted of large leaf spots, many developing at the leaf tips that ranged in size from 1 to greater than 4 cm in diameter. Spots were dark brown to almost black, generally oval to round, visible from both sides of the leaf, and did not exhibit signs of any pathogen. Lesions typically expanded and affected the entire leaf, leaf petiole, and stems, resulting in blight-like symptoms. Severely affected leaves abscised from the plant. In advanced stages of the disease, the foliage of the plant was killed. These symptoms resembled those caused by the sudden oak death (SOD) pathogen, Phytophthora ramorum (3). A Phytophthora sp. was isolated consistently from symptomatic leaf tissue. However, the species was identified as P. citricola based on morphological traits that included the following: production of semipapillate, noncaducous sporangia that were irregular in shape and occasionally had more than one apex; presence of oospores with paragynous antheridia in single culture; and radiate to slightly petaloid colony morphology (1). P. ramorum and other fungi were not recovered. Pathogenicity of four representative isolates was confirmed by gently abrading the adaxial surfaces of attached leaves with a sterile wire brush, placing a colonized agar plug (5 mm in diameter) on the surface, misting the leaf with sterile water, and then covering the plug with a plastic cap that was secured with a wire clip. Control leaves were treated in the same manner but received sterile agar plugs. Plants were maintained in a greenhouse at 23 to 25°C. After 2 days, all leaves inoculated with the isolates exhibited dark brown lesions and by day 6, lesions measured 3 cm in diameter. P. citricola was reisolated from symptomatic lesions. Sterile plug control leaves developed no symptoms. The test was repeated and the results were similar. To our knowledge, this is the first report of P. citricola causing a foliar disease of Pieris japonica in California. P. citricola has been reported as a pathogen on Pieris spp. in Ohio (2). Our finding is important because P. ramorum causes very similar symptoms on this same host (3). The occurrence of these two foliar Phytophthora spp. on this ornamental plant may complicate P. ramorum field detection during inspections and laboratory confirmations as established by quarantine regulations. References: (1) D. C. Erwin and O. K. Ribeiro. Morphology and Identification of Phytophthora Species. Pages 96–144 in: Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (2) W. W. P. Gerlach et al. Phytopathology 64:1368, 1974. (3) P. W. Tooley et al. Plant Dis. 88:993, 2004.
First Report of Bacterial Wilt of Annual Bluegrass Caused by Xanthomonas translucens pv. poae in Montana
Authors: Mitkowski N. A. 
Published: May 2007

During August 2003, a golf course putting green sample composed of Poa annua from the Buffalo Hill Country Club in Kalispell, MT exhibiting symptoms of general decline, wilting, and necrosis was submitted to the University of Rhode Island Turfgrass Disease Diagnostic Laboratory. No pathogenic fungi were observed or cultured from affected plants. Bacterial streaming was observed from cut leaves. Cut leaves were surface disinfested for 5 min in a 0.6% sodium hypochlorite solution and plated on yeast dextrose calcium carbonate (YDC) agar media. A single yellow, mucoid colony type composed of rod-shaped bacteria was isolated from all leaves. Bacteria were gram negative, lacked anaerobic growth, did not fluorescence on King's medium B, and were able to grow at 33°C on YDC. Colonies were transferred to YDC for 10 days, DNA was extracted and a 2,190-bp region encompassing the 16S rRNA, ITS, and 5′ end of the 23S rRNA was amplified via polymerase chain reaction (PCR) using previously published protocols (1). Sequence comparisons of the resulting 2,190-bp PCR product revealed a 99.7% sequence similarity with X. translucens pv. poae (American Type Culture Collection [ATCC] no. 33804) and a 99.8% sequence similarity with X. translucens pv. poae M-1 (Torrington, CT). No higher sequence similarity could be identified from a BLAST search. The Montana isolate and the previously described M-1 isolate were inoculated onto four replicates of 5-month-old P. annua var. annua plants by dipping cut leaves into a bacterial suspension adjusted to 109 CFU/ml in 0.9% NaCl. Control plants were dipped into 0.9% NaCl without the presence of the bacteria. All plants were placed in the greenhouse at an average daytime temperature of approximately 24°C and 12 h of sunlight. After 8 weeks, the plants were assessed for disease and checked for bacterial streaming. This experiment was repeated once. The Montana isolate caused approximately 68 and 70% leaf death and the M-1 isolate caused 21 and 25% leaf death in the two experiments. Bacterial streaming was observed in approximately 50 and 80% of the examined leaves inoculated with the M-1 and Montana isolates, respectively. Control plants showed no leaf mortality or bacterial streaming. Although this pathogen was originally identified in the United States in Michigan (2) and has been prevalent in the northeastern United States for the past 10 to 15 years, to our knowledge, this is the first report of the disease in the northwestern United States. References: (1) N. A. Mitkowski et al. Plant Dis. 89:469, 2005. (2) D. L. Roberts et al. Phytopathology 75:1289, 1985.
First Report of Meloidogyne partityla on Pecan in Florida
Authors: Crow W. T. , Levin R. , Halsey L. A. , Rich J. R. 
Published: May 2007

Pecan (Carya illinoensis (Wagenh.) K. Koch) is an important tree in the southern United States for commercial nut production and residential use. Meloidogyne partityla (pecan root-knot nematode) is only known to parasitize trees in the Juglandaceae including pecan, walnut, and hickory. In the United States, it has been reported on pecan in Texas, New Mexico, and Georgia and internationally in South Africa. Portions of a large pecan nursery in Madison County, Florida were infested with an unknown species of root-knot nematode. The pecan trees at this nursery are grafted onto rootstock and grown in the field until the trees are large enough to sell as bare-root transplant stock in several states including and adjoining Florida. Trees infected by the root-knot nematodes were stunted, had extensive galling and rotting of the root system, and pulled from the ground relatively easily compared with noninfected trees. Attempts to rear these nematodes on tomato in the greenhouse were unsuccessful. Young egg-laying females were isolated directly from pecan roots for speciation. Enzymes extracted from females were resolved with polyacrylamide gel electrophoresis [4% stacking (pH 6.8) and 8% separating gel (pH 8.8) with Tris-glycine buffer]. The esterase (Est) and malate dehydrogenase (Mdh) phenotypes of the unknown Meloidogyne sp. were consistent with those reported for M. partityla. In addition, specimens of M. partityla maintained by the USDA Southeastern Fruit and Tree Nut Research Laboratory in Byron, GA were obtained and their Est and Mdh phenotypes compared with the unknown Meloidogyne sp. under identical electrophoretic and enzyme staining protocols. The Est and Mdh phenotypes of the two isolates were identical. Therefore, we conclude that the root-knot nematodes infesting the pecan nursery in Florida are M. partityla. To our knowledge, this is the first report of this nematode in Florida and also the first report of this nematode from infected nursery stock. It is unknown how long the field has been infested but clearly, there is potential for spread of M. partityla to locations around Florida and bordering states. This nematode may be widespread throughout pecan-growing regions of the United States. References: (1) K. P. N. Kleynhans. Phytophylactica 18:103, 1986. (2) A. P. Nyczepir et al. Plant Dis. 86:441, 2002. (3) J. L. Starr et al. J. Nematol. 28:565, 1996. (4) S. H. Thomas et al. Plant Dis. 85:1030, 2001.
First Report of the Rust Fungus Coleosporium tussilaginis on Tussilago farfara in Turkey
Authors: Tunali B. , Yildirim A. , Aime M. C. , Hernández J. , Berner D. 
Published: May 2007

Tussilago farfara L. (“coltsfoot”, Asteraceae) is indigenous to Eurasia, including Turkey, but has been classified as a Class A noxious weed in North America. Plant pathogens are being sought as potential candidates for biological control of coltsfoot. A rust fungus, Coleosporium tussilaginis (Pers) Lev. has been reported to cause disease on T. farfara from Europe, Russia, and Japan (2). During August of 2001 and 2002, rust pustules were observed on coltsfoot leaves in Kizilcahamam, Ankara, Turkey. Diseased plant specimens with rust pustules were collected, examined microscopically, and compared with published descriptions (1) and specimens in the U.S. National Fungus Collections in Beltsville, MD. The causal agent was subsequently identified as the fungus Coleosporium tussilaginis (Pers.) Lév. on the basis of symptomatology and morphology of uredinial and telial stages such as: clustered, golden-orange, sub-epidermal, erumpent, uredinia approximately 1.0 × 2.0 mm in diameter on the abaxial surface, and corresponding achlorotic areas on the adaxial leaf surfaces; thick-walled (3.0 to 4.0 μm), pale orange, coarsely verrucose, variously shaped (mostly broadly ellipsoid or angular) urediniospores measuring 23.5 to 33.0 × 20.0 to 25.7 μm (average = 27.3 × 23.3 μm), with obscure and scattered germ pores; and orange-red telia on the abaxial leaf surfaces covered by a gelatinous apical layer enclosing orange-red, cylindrical to clavate teliospores measuring 50.0 to 80.0 × 18.0 to 28.0 μm. A voucher specimen of this material has been deposited in the U.S. National Fungus Collections (BPI 863516). To the best of our knowledge, this is the first report of C. tussilaginis rust on coltsfoot in Turkey. References: (1) G. Deml et al. Phytopathol. Z 104:39, 1982. (2) D. F. Farr et al. Fungal Databases. USDA-ARS Systematic Botany and Mycology Laboratory, Online publication. ARS, USDA, 2005.
Phytophthora ramorum on Quercus ilex in the United Kingdom
Authors: Denman S. , Kirk S. A. , Brasier C. M. , Barton V. C. , Hughes K. J. D. , Webber J. F. 
Published: May 2007

Phytophthora ramorum causes bleeding cankers of trunks of trees native to the west coast of the United States (i.e., Quercus kelloggii, Q. parvula var. shrevei, and Lithocarpus densiflorus). In the United Kingdom so far, bleeding cankers caused by inner bark infections have been found on Aesculus hippocastanum, Fagus sylvatica, Q. cerris, Q. falcata, and Q. petraea ( http://rapra.csl.gov.uk [2005]). Shoot tip dieback (ramorum dieback) and foliar necrosis (ramorum leaf blight) are other diseases caused by the pathogen on understory and ornamental plants (3). Inoculum is produced on infected shoots and leaves of foliar hosts but not on bole cankers (1). Foliar hosts are thus critical in initiating and maintaining epidemics of tree mortality resulting from lethal bark cankers. Ramorum dieback and blight occurs in Europe on genera Rhododendron, Camellia, Kalmia, Pieris, and Viburnum (http://rapra.csl.gov.uk [2005]), and now we report these diseases on foliage and shoots of holm oaks (Quercus ilex) in Cornwall (UK). First discovered in November 2003, infected young leaves had a water-soaked, dull gray appearance, and petioles were blackened. Lesions started at leaf margins, tips, or petioles, often progressing into the midrib veins. Initial infections also occurred on shoots and extended into the petioles. If shoots were infected, they were blackened at first, but later in the season clusters of dry, dead leaves and twigs characterized branch tips. Infected mature leaves bore dry, reddish-brown, restricted lesions. P. ramorum (A1 sexual compatibility type belonging to the European population) was isolated and confirmed by morphological studies, ITS sequence (GenBank Accession No. AY924253), and amplified fragment length polymorphism analyses. Lesions developed on detached leaves dipped for 10 sec in inoculum (4 × 105 zoospores per ml) and incubated in moist chambers at 20°C for 6 days (2). Two isolates were used (four leaves per isolate). The pathogen was reisolated, and the tests were repeated twice. Koch's postulates were also successfully completed once on foliage attached to saplings. To our knowledge, this is the first report of P. ramorum on holm oak. So far, at least 24 holm oaks are infected at various woodland and garden sites in the United Kingdom; infected rhododendrons have also been found at these sites. P. ramorum has also been recorded on saplings in nurseries. The high sporulation potential, the evergreen nature of leaves, and susceptible shoots indicate that holm oak could be a significant source of inoculum for other hosts. References: (1) J. M. Davidson et al. Phytopathology 95:587, 2005. (2) S. Denman et al. Plant Pathol. 54:512, 2005. (3) E. M. Hansen et al. Plant Dis. 89:63, 2005.
Iris yellow spot virus: A New Onion Disease in Spain
Authors: Córdoba-Sellés C. , Martínez-Priego L. , Muńoz-Gómez R. , Jordá-Gutiérrez C. 
Published: May 2007

So far, only three viral diseases have been identified in onion crops grown in Spain. These are Tomato spotted wilt virus (TSWV), Onion yellow dwarf virus (OYDV), and Leek yellow stripe virus (LYSV). In September 2003, unusual virus-like symptoms including straw-colored, dry, tan, diamond-shaped lesions on the leaves and stalks, sometimes with necrotic lesions, curled leaves, and bulbs of reduced size, were observed on several onion plants (Allium cepa L.) in commercial fields in Albacete, Spain. Severely affected plants eventually died. To verify the identity of the disease found in the Spanish onions, double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was performed on leaf extracts of symptomatic onions using specific polyclonal antibodies against OYDV, LYSV, Cucumber mosaic virus (CMV) (Biorad Phyto-Diagnostics, Marnes-La Coquette, France), Iris yellow spot virus (IYSV), and TSWV (Loewe Biochemica, Sauerlach, Germany). All samples of infected onion tissue were positive for IYSV and negative for the other viruses tested. To confirm the ELISA results, viral RNA was extracted from five of the ELISA-positive onion samples, a healthy onion plant, and a positive control for IYSV (DSMZ, Braunschweig. Germany). The extracted RNA was used in a One-Step reverse transcription-polymerase chain reaction (RT-PCR) assay using SuperScript Platinum Taq (Invitrogen Life Technologies, Barcelona, Spain) in the presence of the IYSV1S and IYSV1A primers for the nucleocapsid gene of IYSV (1). The RT-PCR assay produced an amplicon of the expected size of 790 bp. No amplification products were observed when healthy plants or a water control were used as templates in the RT-PCR reaction. To establish the authenticity of the virus from onion, the PCR products were purified (High Pure PCR Product Purification Kit, Roche Diagnostics, Mannheim, Germany), sequenced, and the nucleotide sequences obtained were analyzed and compared with the published sequences in GenBank. The PCR product was 97% identical to the sequence of the IYSV nucleocapsid gene (Genbank Accession No. AB121026). IYSV, an emerging tospovirus that is potentially a devastating pathogen of onion, has been reported in many locations in Brazil, Japan, the Netherlands, Israel, Australia, the western United States, Slovenia, and Iran (2). IYSV is included in the European and Mediterranean Plant Protection Organization alert list of viruses (2), and to our knowledge, this is the first report of IYSV in Spain. This tospovirus is transmitted in a propagative manner by Thrips tabaci. Although the vector is present in large populations in the onion-growing areas in Spain, the efficiency of the Mediterranean ecotype in transmitting IYSV is not known. References: (1) B. A. Coutts et al. Australas. Plant Pathol. 32:555, 2003. (2) European and Mediterranean Plant Protection Organization. EPPO on-line publication at www.eppo.org/QUARANTINE/Alert_List/Viruses/irysxx.html .
First Report of Tomato chlorosis virus and Tomato infectious chlorosis virus in Tomato Crops in France
Authors: Dalmon A. , Bouyer S. , Cailly M. , Girard M. , Lecoq H. , Desbiez C. , Jacquemond M. 
Published: May 2007

Since 2002, yellowing symptoms associated with high levels of white-fly populations have been observed in plants of protected tomato crops in France. Symptomatic plants exhibited interveinal yellowing areas in older leaves, followed by generalized yellowing. Symptoms were not observed in young plants or fruits. Trialeurodes vaporariorum populations were generally abundant in spring, and Bemisia tabaci (established in France for approximately 10 years) became predominant in summer and fall. To check for the presence of Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV), two whitefly-transmitted criniviruses known to induce yellowing symptoms, 696 samples were collected in the major tomato-growing areas; 573 samples from southern France and 123 samples from northern France. Total RNA was extracted from each sample and analyzed using reverse transcription-polymerase chain reaction (RT-PCR). Primers specific to ToCV (2) and TICV (1,3) were used to amplify either part of the heat-shock-like protein gene HSP70h (both viruses) or part of the diverged coat protein gene (CPd), (TICV only). A 439-bp DNA fragment was obtained with ToCV primers in 178 samples from southern France collected mainly from mid-spring to early fall from 2002 to 2004. Three RT-PCR products amplified from samples collected from diverse growing areas were sequenced and showed 99 to 100% sequence identity with published ToCV sequences from Spain (GenBank Accession Nos. AF215818, AF233435, and AF215817), Portugal (GenBank Accession No. AF234029), Sicily (GenBank Accession No. AY048854), and the United States (GenBank Accession No. AF024630). Considering the high frequency of ToCV-infected samples (41 positive samples of 112 samples collected in 2002, 71 of 295 collected in 2003, and 66 of 166 collected in 2004), this virus appears to be well established in southern France but remains absent in the northern regions. The presence of TICV was tested in 485 samples using the CPd-specific primers or the HSP70h-specific primers. The virus was detected in only two samples from Nice (southeastern France) in 2003 with both primer pairs. The CPd DNA fragment (700 bp) from one of these samples was sequenced, showing 98.9% sequence identity with a TICV Japanese isolate (AB085603). Results of these assays suggest that in contrast to ToCV, TICV is not yet broadly established in France. This difference could be associated with the specificity of the vectors, since ToCV is transmitted by B. tabaci and T. vaporariorum, while TICV is transmitted only by T. vaporariorum (4). References: (1) R. H. Li et al. Plant Dis. 82:84, 1998. (2) D. Louro et al. Eur. J. Plant Pathol. 1065:589, 2000. (3) A. M. Vaira et al. Phytoparasitica 30:290, 2002. (4) G. C. Wisler et al. Plant Dis. 82:271, 1998.
First Report of Apple Rot Caused by Neofabraea alba in Chile
Authors: Henriquez J. L. 
Published: May 2007

A new rot of stored apples was observed in local and export markets on apples that were grown in the south-central region of Chile during 2004. Circular, pale brown spots with a darker outer ring were observed at least 3 months after harvest in cvs. Braeburn, Fuji, Granny Smith, Pink Lady, and Royal Gala. Lesions developed from lenticel cavities or wounds and reached 2 to 3 cm in diameter after 1 week at room temperature. Symptoms resembled those produced by the bull's eye rot pathogens on apple that occur in other parts of the world. Acervuli developed in the rotted areas, and microscopic examination revealed the presence of the fungus Neofabraea alba (Guthrie) (anamorph Phlyctema vagabunda Desm.) characterized by production of curved macroconidia and absence of microconidia (1). Pure culture on potato dextrose agar (PDA) yielded a characteristic white sterile mycelium. Four cv. Pink Lady apples were wound inoculated with mycelium of the pathogen and four apples were wound inoculated with a 5 × 104 CFU/ml conidial suspension. Four apples were wounded and inoculated with sterile water as a negative control. The fruit was held at 20°C. Symptoms appeared after 4 and 5 days in the mycelium and conidial inoculated apples, respectively. Resulting symptoms were similar to those originally observed. Abundant macroconidia were produced at the inoculation sites, and a sterile, white mycelium was recovered after isolation on PDA. To our knowledge, this is the first report of apple fruit rot caused by N. alba in Chile. Reference: (1) J. L. Henriquez et al. Plant Dis. 88:1134, 2004.