bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prospective, randomised clinical trial on the necessity of using a silicone intubarium in the context of a dacryocystorhinostomy (DCR) in patients with postsaccal lacrimal duct stenosis
Authors: Bahner Lia , Zebralla Veit , Dietz Andreas , Otto Mathias , Pirlich Markus 
Published: March 2024

Abstract
Objective.
This study evaluates the effect of a silicone stent tube (SST) on the success rate of endonasal endoscopic dacryocystorhinostomy (EN-DCR) to treat primary acquired nasolacrimal duct obstruction (PANDO).
Design.
Prospective randomized clinical trial.
Methods.
Patients were randomly assigned to receive EN-DCR with or without SST intubation over a period of 3 months. The surgery was performed using standardized techniques. Patients were assessed at three different timepoints: one day, 12 weeks and 24 weeks after the surgery. The results were compared in order to evaluate statistical differences. Surgical success was determined by means of positive irrigation procedures, as well as by the improvement of symptoms and a high level of patient satisfaction.
Results.
A total of 56 randomized cases completed 24 weeks of follow up. 1 Patient dropped out due to malignant genesis of the nasolacrimal duct obstruction (NLDO). After 24 weeks of follow up no statistically significant differences in levels of epiphora (p > .10) or patency (p > .16) were revealed. Comparisons regarding changes in time did not show levels of significance (p > .28).
Conclusion.
This study could not confirm a statistically significant benefit or disadvantage for SST Insertion in DCR Surgery.
Anti-proliferative and Apoptotic Efficacy of Nano-PLGA encapsulated Quercetin Molecules by down-regulation of Akt in K-ras mutated NSCLC cell lines, A549 and H460
Authors: Mukherjee Avinaba , Ghosh Sandip , Ganguli Sayak , Basu Biswarup 
Published: March 2024

Abstract
To test if encapsulating hydrophobic flavonoids in nanoparticles could offer a new possibility in the therapeutics of non-small cell lung cancer (NSCLC), quercetin was encapsulated in PLGA nanoparticles by solvent displacement technique. The synthesised nanoparticles were then characterised by dynamic light scattering (DLS), Fourier transforms infrared spectroscopy (FTIR), and atomic force microscopy (AFM). The size of the nanoparticles with smooth surface topology was estimated at 110 nm. Treatment with nano-PLGA encapsulated quercetin (NPEQ) triggered the death of K-ras mutated NSCLC cells, A549 and H460, and showed 50% cell cytotoxicity in them at a dose of 406 ng/ml and 306 ng/ml, respectively. NPEQ was able to block uncontrolled cell proliferation by inducing concomitant destruction of BrdU activity and a lower incidence of cell migrations. Cell death was due to the induction of apoptosis rather than necrosis, as revealed by morphological alterations and phosphatidylserine externalisation induced by NPEQ. NPEQ also caused the arrest of A549 and H460 cells at the sub-G1 stage. NPEQ induced down-regulation of Akt, which is usually found to be hyperactive in NSCLC due to K-ras mutation. This indicates that NPEQ caused target-specific apoptotic and antiproliferative activity by targeting the downregulation of Akt. Further, when NPEQ was generated in the tumour-bearing mice model, it showed antitumor efficacy. Besides this, histological alteration of tissue architecture and modulation of an apoptotic marker protein in mice indicates the prospect and advantages of nanoparticulate quercetin delivery in therapeutic formulations against cancer.
Effect of rehabilitation on renal outcomes after acute kidney injury associated with cardiovascular disease: a retrospective analysis
Authors: Hirano Yuma , Fujikura Tomoyuki , Kono Kenichi , Yamaguchi Tomoya , Ohashi Naro , Yokoyama Yurina , Toda Masahiro , Yamauchi Katsuya , Yasuda Hideo 
Published: March 2024

Abstract
Background: Acute kidney injury (AKI) incidence is extremely high worldwide, and patients who develop AKI are at increased risk of developing chronic kidney disease (CKD), CKD progression, and end-stage renal disease (ESRD). However, there is no established treatment strategy for AKI. Based on the idea that exercise stabilizes hemodynamics, we hypothesized that rehabilitation would have beneficial renal outcomes after AKI associated with cardiovascular disease. Therefore, the purpose of this study was to determine whether rehabilitation can stabilize hemodynamics and positively impact renal outcomes in patients with AKI associated with cardiovascular disease.
Methods: In total, 107 patients with AKI associated with cardiovascular disease were enrolled in this single-center retrospective study and were either assigned to the intervention group (n = 36), which received rehabilitation at least once a week for at least 8 consecutive weeks, or to the control group (n = 71). Estimated glomerular filtration rate was assessed at baseline before admission, at the lowest value during hospitalization, and at 3, 12, and 24 months. Trends over time (group × time) between the two groups were compared using generalized estimating equations. Moreover, congestive status was assessed by amino-terminal pro-B-type natriuretic peptide (NT-proBNP), and the effect of rehabilitation on congestion improvement was investigated using logistical regression analysis.
Results: The time course of renal function after AKI, from baseline to each of the three timepoints suggested significant differences between the two groups (p < 0.01). However, there was no significant difference between the two groups at any time point in terms of percentage of patients with a high risk of developing ESRD. Moreover, the presence or absence of intervention was significantly associated with improvement in congestion at 3 months (p < 0.05, odds ratio: 0.300, 95% confidence interval: 0.092–0.972).
Conclusion: Our results suggest that rehabilitation after AKI associated with cardiovascular disease correlates with an improvement in congestion and may have a positive effect on the course of renal function after AKI.
DMD-based Spatiotemporal Superresolution Measurement of a Supersonic Jet using Dual Planar PIV and Acoustic Data
Authors: Kaneko Sayumi , Delpozo Alvaro , Nishikori Hiroki , Ozawa Yuta , Nonomura Taku 
Published: March 2024

Abstract

The present study applies a framework of the spatiotemporal superresolution measurement based on the total-least-squares dynamic mode decomposition (tlsDMD), the Kalman filter and the Rauch-Tung-Striebel smoother to an axisymmetric underexpanded supersonic jet of a jet Mach number of 1.35. Dual planar particle image velocimetry (dual PIV) was utilized, and paired velocity fields of the flow with a short time interval were obtained at a temporal resolution of 5000 Hz. High frequency acoustic data of 200,000 Hz were simultaneously obtained. Then, the time-resolved velocity fields of the supersonic jet were reconstructed at a temporal resolution of 200,000 Hz. Also, time coefficients of dynamic modes in high temporal resolution were calculated. The correlation between time coefficients implies that the mixing promotion by screech tone causes the lift-up of the high velocity fluid from the jet center and accelerates at the downstream side.
Single-cell infrared absorption spectroscopy of bacterial inclusion bodies
Authors: Duverger Wouter , Tsaka Grigoria , Khodaparast Ladan , Khodaparast Laleh , Louros Nikolaos , Rousseau Frederic , Schymkowitz Joost 
Published: March 2024

Abstract
Background
Inclusion bodies (IBs) are well-known subcellular structures in bacteria where protein aggregates are collected. Various methods have probed their structure, but single-cell spectroscopy remains challenging. Atomic Force Microscopy-based Infrared Spectroscopy (AFM-IR) is a novel technology with high potential for the characterisation of biomaterials such as IBs.
Results
We present a detailed investigation using AFM-IR, revealing the substructure of IBs and their variation at the single-cell level, including a rigorous optimisation of data collection parameters and addressing issues such as laser power, pulse frequency, and sample drift. An analysis pipeline was developed tailored to AFM-IR image data, allowing high-throughput, label-free imaging of more than 3500 IBs in 12000 bacterial cells. We examined IBs generated in Escherichia coli under different stress conditions. Dimensionality reduction analysis of the resulting spectra suggested distinct clustering of stress conditions, aligning with the nature and severity of the applied stresses. Correlation analyses revealed intricate relationships between the physical and morphological properties of IBs.
Conclusions
Our study highlights the power and limitations of AFM-IR, revealing structural heterogeneity within and between IBs. We show that it is possible to perform quantitative analyses of AFM-IR images over a large collection of different samples and determine how to control for various technical artefacts.
A multidimensional analysis of MRPL17 protein in human tumors
Authors: Li Huan , Xie Xuewen , Zhang Liang , Fei Shuke 
Published: March 2024

Abstract
MRPL17 is one of mitochondrial ribosome protein (MRP) family proteins, which have oncogenic effects in several malignant tumors. However, it is unclear that the relationship between MRPL17 expression pattern and prognosis across different cancer types. Also, the biological function or effects on the immune microenvironment of MRPL17 is unknown. In this study, we parsed multiple public databases to explore the potential tumorigenic actions of MRPL17, including correlations with prognosis, microsatellite instability (MSI), tumor mutational burden (TMB), immune checkpoint genes, immune cell infiltration, and immunotherapy response in pan-cancer. Moreover, we validated MRPL17 expression in a tissue microarray by immunohistochemistry. The results showed that MRPL17 was upregulated in 19 cancer types and correlated with poor prognosis in many cancers. The correlation between MRPL17 and TMB was found in 10 cancers as well as MSI in five. The expression level of MRPL17 was found to be notably correlated with immune cell infiltration, showing a negative correlation with CD4 T cells infiltration and a positive correlation with dendritic cells (DC). MRPL17 expression levels were positively associated with drug sensitivity in certain cancers. In addition, we discovered that MRPL17 participated in the DNA repair at the single-cell level for most cancers. These findings provides a promising candidate for therapeutic targets and a new direction for future research.
Intracranial volumetric evaluation in postnatally repaired myelomeningocele infants
Authors: Hashimoto Hiroaki , Irizato Naoki , Takemoto Osamu , Chiba Yasuyoshi 
Published: March 2024

Abstract

Introduction
Most myelomeningocele (MMC) cases exhibit ventriculomegaly or hydrocephalus, yet a comprehensive volumetric assessment of MMC intracranial structures is lacking. This study aims to provide baseline data on intracranial structural volume immediately after birth in postnatally repaired MMC infants.
Methods
In this retrospective single-center study, we analyzed 52 MMC infants undergoing postnatal closure, utilizing head computer tomography scans at birth for volumetric assessment. Intracranial volume (ICV), lateral ventricles volume (LVV), choroid plexus volume (CPV), and posterior cranial fossa volume (PCFV) were measured. Comparative analysis with healthy controls (eight infants at 0 months) employed the Wilcoxon rank-sum test. Receiver operating characteristic (ROC) analysis discriminated cases with and without ventriculoperitoneal shunt (VPS).
Results
MMC infants exhibited significant differences compared to healthy cases in LVV (33.18 mL vs. 2.05 mL, p < 0.001), CPV (0.67 mL vs. 0.25 mL, p = 0.005), and PCFV (21.35 mL vs. 39.50 mL, p < 0.001), but not in ICV (407.50 mL vs. 418.15 mL, p = 0.25). Thirty-seven cases (71.15%) underwent VPS. ROC analysis revealed an LVV cut-off value of 6.74 mL for discriminating cases with and without VPS.
Conclusion
Postnatally repaired MMC infants showed significantly larger LVV and CPV, and smaller PCFV compared to healthy infants, providing crucial baseline data for volumetric evaluation. These findings enhance our understanding of intracranial volumetric changes in MMC, facilitating more objective assessments of MMC cases.
Comparing and evaluating realistic wood structure models derived from multi-source remote sensing data: A focus on suitability reinforcement
Authors: Shi Yanru , Guo Ming , Liang Xuanshuo , Zhao Jiawei , Shang Xiaoke , Huang Ming , Guo Shuai 
Published: March 2024

Abstract

Exploring the comparison and evaluation of wood structure realistic model based on the fusion of multi-source remote sensing data and suitability reinforcement effects, aiming to proactively and accurately prevent potential structural issues to ensure long-term effective preservation of historical buildings. Various point cloud data from different sources were collected using equipment such as unmanned aerial vehicle (UAV) and LiDAR; Based on the point cloud data, a hybrid realistic model was established, using the existing point cloud data to successfully restore the orientation angle of the mortise and tenon interface, the angle was determined to further enhance the real reflection of the existing shape of the column-architrave structure, which provides a more accurate analysis of the structural analysis of the stress model; Based on the establishment of a realistic model, precise identification of weak locations was carried out and two different types of reinforcement were designed. A comparative analysis of the effectiveness of these two types of reinforcement was carried out to determine the most suitable reinforcement scheme applicable to the weakened area. The results show that by performing finite element analysis on the 24 columns in the realistic model, the columns with weak areas—M2W23 columns, were found to present the largest deformation of 4.65 mm in the upper and middle parts of the columns. Of the two reinforcement types, the reinforcement with aluminum alloy material reduced the deformation of the wooden columns from 4.65 mm to 3.5 mm, and after being reinforced, the deformation of the wooden columns was reduced by about 24.7% compared to that before the reinforcement. In comparison, the deformation of the wooden columns was reduced from 4.65 mm to 2.8 mm after reinforcement with different thicknesses of CFRP (Carbon Fiber Reinforced Plastic) sheet, which is a 39.7% reduction relative to the unreinforced condition, showing better stability. Reinforcement with different thicknesses of CFRP sheets provides flexibility to cope with the unequal damage of the wooden columns, thus ensuring an equal distribution of strength throughout the columns. The results indicate that within this structure, different thicknesses of CFRP sheets reinforcement exhibit more suitable performance.
Studies on annual life history of okra shoot and fruit borer, Earias vittella (Fabricius) on okra under mid hills of Himachal Pradesh
Authors: Kumar Manjeet , Thakur Anjana , Motmayen Mohammad Ilyas , Mehta Vasu 
Published: March 2024

Abstract
Annual life cycle of Earias vittella (Fabricius) was studied under laboratory conditions at mid-hills of Himachal Pradesh during 2021-22. Five overlapping generations of E. vittella were observed in a year. The mean duration of generations ranged between 31.6 to 34.6 days in generations falling in June to October months while its development slowed from November to May. Mean duration of egg, larva and pupa was 3.4 ± 0.05, 14.0 ± 0.44 and 8.30 ± 0.24 days, respectively. Mean developmental period excluding adult longevity was 26.8 ± 0.72 days in generations from June to October. The longevity of adult male and female was 5.3 ± 0.10 and 6.1 ± 0.20 days, respectively, while the average fecundity was 147.8 ± 21.60. E. vittella showed maximum mortality at larval stage (51.36%).
Correlation Between Higher Lateral Tibial Slope and Inferior Long Term Subjective Outcomes Following Single Bundle Anterior Cruciate Ligament Reconstruction
Authors: Hung Yu-Chieh , Chuang Chieh-An , Yao Shang-Yu , Lin Keng-Yi , Hung Shih-Feng , Chen Yi-Jou , Chiu Chi-Hao , Ho Chin-Shan , Chan Yi-Sheng , Yang Cheng-Pang 
Published: March 2024

Abstract

Background The impact of anatomical factors, such as the lateral tibial slope (LTS), on outcomes following anterior cruciate ligament (ACL) reconstruction is an area of growing interest. This study was led by the observation that patients with a higher LTS may have different recovery trajectories. Hypothesis/Purpose: The purpose of this study was to investigate the correlation between a higher LTS and long term inferior subjective outcomes following single-bundle ACL reconstruction. Study Design: This study was designed as a retrospective cohort study. Methods The study comprised 138 patients who underwent single-bundle ACL reconstruction. The LTS was measured on preoperative radiographs. Patient-reported outcome measures (PROMs) were collected, which included the Lysholm Knee Score, UCLA Activity Score, IKDC Score, and Tegner Activity Score, over a mean follow-up duration of 137 months. Results A significant negative correlation was found between LTS and all measured PROMs (p < 0.001). The established cut-off value of LTS distinguishing between "Good" and "Fair" Lysholm scores was 8.35 degrees. Female patients has statistically significant higher LTS and lower PROMs scores than male. Patients with LTS greater than or equal to 8.35 had significantly lower PROMs, indicative of poorer functional and subjective outcomes. Conclusion Our findings suggest that a higher LTS is associated with inferior subjective outcomes following single-bundle ACL reconstruction in long term. The LTS cut-off value of 8.35 degrees could potentially be used as a reference in preoperative planning and patient counseling. Clinical Relevance: Understanding the relationship between LTS and ACL reconstruction outcomes could inform surgical planning and postoperative management. These findings highlight the need to consider anatomical variances, such as LTS, when assessing patient-specific risks and recovery expectations, contributing to the advancement of personalized care in sports medicine.
Employing supramolecular chemistry strategies for the separation of mixtures of anisole and bromoanisole isomers
Authors: Barton Benita , Senekal Ulrich 
Published: March 2024

Abstract

In the present work, host compounds trans-9,10-dihydro-9,10-ethanoanthracene-11,12-dicarboxylate (H1), trans-α,α,α’,α’-tetraphenyl-9,10-dihydro-9,10-ethanoanthracene-11,12-dimethanol (H2) and trans-α,α,α’,α’-tetra(p-chlorophenyl)-9,10-dihydro-9,10-ethanoanthracene-11,12-dimethanol (H3) were assessed for their host ability for anisole (ANI) and 2-, 3- and 4-bromoanisole (2-, 3- and 4-BA). It was demonstrated that H3 formed complexes with each of these guest species, while H1 and H2 only possessed the ability to enclathrate ANI. When H3 was crystallized from equimolar binary guest solutions, a significantly enhanced host affinity was observed for ANI and 3-BA. As examples, equimolar binary ANI/2-BA and 2-BA/3-BA solutions afforded complexes that demonstrated a near-complete H3 selectivity towards ANI (97.5%) and 3-BA (94.5%), respectively. Furthermore, from H3 crystallization experiments in binary ANI/2-BA, ANI/4-BA, 3-BA/2-BA and 3-BA/4-BA mixtures, where the molar guest amounts were varied sequentially, were calculated significant K values, so much so that H3 may be used to separate very many of the guest anisole mixtures prepared in this work, through supramolecular chemistry strategies, which serves as a greener separation protocol compared with tedious and energy intensive fractional distillations. Thermal analyses were also used to investigate the relative stabilities of each of the single solvent complexes.
Global Perspectives on the Management of Primary Progressive Aphasia
Authors: Gallée Jeanne , Cartwright Jade , Grasso Stephanie , Jokel Regina , Lavoie Monica , McGowan Ellen , Pozzebon Margaret , Beber Bárbara Costa , Duboisdindien Guillaume , Montagut Núria , Norvik Monica , Sugimoto Taiki , Townsend Rosemary , Unger Nina , Winsnes Ingvild E. , Volkmer Anna 
Published: March 2024

Abstract

Speech-language therapists/pathologists (SLT/Ps) are key professionals in the management and treatment of primary progressive aphasia (PPA), however, there are gaps in education and training within the discipline, with implications for skills, confidence, and clinical decision-making. This survey aimed to explore the areas of need amongst SLT/Ps working with people living with PPA (PwPPA) internationally to upskill the current and future workforce working with progressive communication disorders. One hundred eighty-five SLT/Ps from 27 countries who work with PwPPA participated in an anonymous online survey about their educational and clinical experiences, clinical decision-making, and self-reported areas of need when working with this population. Best practice principles for SLT/Ps working with PwPPA were used to frame the latter two sections of this survey. Only 40.7% of respondents indicated that their university education prepared them for their current work with PwPPA. Competency areas of “Knowing people deeply,” “Practical issues,” “Connectedness,” and “Preventing disasters” were identified as the basic areas of priority and need. Respondents identified instructional online courses (92.5%), sample tools and activities for interventions (64.8%), and concrete training on providing care for advanced stages and end of life (58.3%) as central areas of need in their current work. This is the first international survey to comprehensively explore the perspectives of SLT/Ps working with PwPPA. Based on survey outcomes, there is a pressing need to enhance current educational and ongoing training opportunities to better promote the well-being of PwPPA and their families, and to ensure appropriate preparation of the current and future SLT/P workforce.
Incidence of degenerative disease of the temporomandibular joint in anterior open bite patients with different molar relationships: a retrospective CBCT study
Authors: Wang Chenxu , Guo Feng , Hu Hengyu , liu Chenghuan , Lei Lang 
Published: March 2024

Abstract
Objectives
To assess the prevalence of degenerative joint disease (DJD) in anterior open bite (AOB) patients with different molar relationships.
Methods
246 AOB adult orthodontic patients were divided into Class I group (n = 65), Class II group (n = 116) and Class III group (n = 65) based on their sagittal molar relationships, meanwhile, 70 normal subjects were selected as control group. The morphological characteristics of temporomandibular joint (TMJ) and occurrence of DJD in the subjects were detected by cone-beam computed tomography, and the incidence of DJD among them was compared. Mandibular plane angle and the vertical distance between the upper and lower incisors (ULD) were also measured.
Results
The overall incidence of DJD in AOB patients and control group was 30.28% and 5.00%, respectively. Class II group showed a significantly highest incidence of DJD (50.43%, P<0.001), highest frequency of bilateral DJD (43.95%, P<0.001) and highest mandibular plane angle (MP-SN of 44.39°±7.02° and MP-FH of 35.68° ± 6.74°,P<0.001). Among the AOB groups, Class III group exhibited the highest ULD (3.35 ± 1.86mm, P<0.05) but the lowest incidence of DJD (10.77%).
Conclusions
AOB patients with Class II molar relationship exhibit the highest incidence of DJD, and orthodontists should pay closer attention to the risk of temporomandibular joint diseases (TMD) in Class II AOB patients during clinical practice.