bluuebunny/crossref_metadata_2025_split huggingface dataset:
“One Method to Label Them All”: A Single Fully Automated Protocol
for GMP-Compliant 68Ga Radiolabeling of PSMA-11, Transposable to
PSMA-I&T and PSMA-617
Authors: Fouillet Juliette , Donzé Charlotte , Deshayes Emmanuel , Santoro Lore , Rubira Léa , Fersing Cyril 
Published: March 2024

Background::
Prostate-specific membrane antigen (PSMA) is an ideal target for molecular
imaging and targeted radionuclide therapy in prostate cancer. Consequently, various
PSMA ligands were developed. Some of these molecules are functionalized with a chelator that
can host radiometals, such as 68Ga for PET imaging. The 68Ga radiolabeling step benefits from
process automation, making it more robust and reducing radiation exposure.

Objective::
To design a single automated radiolabeling protocol for the GMP-compliant preparation
of [68Ga]Ga-PSMA-11, transposable to the production of [68Ga]Ga-PSMA-617 and
[68Ga]Ga-PSMA-I&T.

Methods::
A GAIA® synthesis module and a GALLIAD® generator were used. Radio-TLC and
radio-HPLC methods were validated for radiochemical purity (RCP) determination. Three
[68Ga]Ga-PSMA-11 validation batches were produced and thoroughly tested for appearance and
pH, radionuclide identity and purity, RCP, stability, residual solvent and sterility. Minimal modifications
were made to the reagents and disposables for optimal application to other PSMA ligands.

Results::
[68Ga]Ga-PSMA-11 for clinical application was produced in 27 min. The 3 validation
batches met the quality criteria expected by the European Pharmacopoeia to allow routine production.
For optimal transposition to PSMA-617, the solid phase extraction cartridge was
changed to improve purification of the radiolabeled product. For application to PSMA-I&T, the
buffer solution initially used was replaced by HEPES 2.7 M to achieve good radiochemical
yields. Residual HEPES content was checked in the final product and was below the Ph. Eur.
threshold.

Conclusion::
A single automated radiolabeling method on the GAIA® module was developed
and implemented for 68Ga radiolabeling of 3 PSMA ligands, with slight adjustments for each
molecule.
MOLECULAR IDENTIFICATION OF A LOCAL LACTIC ACID BACTERIA (LAB) ISOLATE (B21) AND PRIMER CONFIRMATION FOR D-Lactate Dehydrogenase (D-LDH) GENE ISOLATION
Authors: Madhani Anisa Tiara , Nurcahyo Heru , Octavia Bernadetta , Astuti  , Mercuriani Ixora Sartika 
Published: February 2024

This study aims to identify a lactic acid bacteria (BAL) isolated from native chicken (Gallus gallus domesticus) intestinum (B21) based on 16S rRNA gene sequence and also to confirm primers for PCR-based D-Lactate dehydrogenase (D-LDH) gene isolation. The genomic DNA of B21 was isolated then amplified using 16S rRNA. The PCR product then sequenced and aligned. The sequencing was done in 1st Base Pte. Malaysia. The sequence then aligned using the Basic Local Alignment Search Tool (BLAST) in NCBI to know the similar genome species. Isolation D-LDH gene was done through PCR using 5 primers (idb 0101, idb 1147, idb 0813, idb 1010, and idb 2021) of B21 and also J15 isolate genome. The PCR product were then sequenced and aligned with the D-LDH gene sequences in NCBI.
The results of molecular identification based on 16S rRNA markers showed that B21 has 99.45% similarity to Bacillus proteolyticus. Amplification of the LDH gene with idb 1010 primer on J15 isolate yielded 4 fragments (i.e. 650, 1400, 1500, and 1700bp). The results of phylogenetic tests showed that the 1700bp fragment has a high similarity to the D-LDH gene in Myobacterium tuberculosis species with a bootstrap value of 84%. While other size fragments cannot be trusted to have similarity to LDH sequence because they have not reached the minimum bootstrap value that meets the qualifications.
Pelatihan Pembuatan Soal dan Penilaian Daring Bagi Guru SMP dan SMA di Desa Lembo Kabupaten Konawe Utara
Authors: Patih Tandri , Sangila Muhammad Syarwa , Boy La , Masdin Masdin , Kadir Abdul , Halistin Halistin , Nurhalizah Dina 
Published: February 2024

Sebagai seorang pendidik, guru berkewajiban untuk meningkatkan kualitas dari proses pembelajaran, yang kemudian berdampak pada peningkatan kemampuan peserta didiknya. Sehingga, guru dituntut untuk terus mengembangkan kompetensi yang dimilikinya, salah satunya yaitu kompetensi profesionalisme. Kegiatan pengabdian kepada masyarakat (PKM) ini bertujuan untuk menambah wawasan dan meningkatkan kompetensi guru SMP dan SMA di Desa Lembo Kabupaten Konawe Utara melalui pelatihan pembuatan soal dan penilaian secara daring (dalam jaringan). Di era digitalisasi saat ini pemanfaatan teknologi informasi (IT) diberbagai sektor/bidang menjadi begitu intens, tidak terkecuali dibidang Pendidikan. Lembaga Pendidikan dituntut untuk dapat melaksanakan proses pembelajaran hingga proses penilaian dengan memanfaatkan teknologi informasi. Terutama dalam menghadapi kondisi pandemi, yang menuntut dilaksanakannya proses pembelajaran dan penilaian secara daring. Kegiatan PKM ini dilaksanakan dalam tiga tahapan, yaitu perencanaan, pelaksanaan, dan evaluasi. Metode yang digunakan dalam kegiatan PKM ini yaitu pelatihan, pendampingan, dan diskusi. Pelatihan dilaksanakan dengan memberikan informasi mengenai pembuatan soal dan penilaian secara daring. Materi yang disampaikan mencakup kebijakan pelaksanaan penilaian daring sebelum dan selama pandemi serta pembuatan soal dan penilaian daring menggunakan google form. Hasil yang dicapai dalam Kegiatan PKM ini, yaitu: 1) meningkatnya pengetahuan dan pemahaman guru tentang pemanfaatan google form sebagai media dalam melaksanakan penilaian secara daring; 2) meningkatnya motivasi guru dalam mengembangkan kompetensi pedadogiknya. Hasil PKM ini juga memperlihatkan tingkat kepuasan yang baik dari guru-guru peserta pelatihan, dengan persentase sangat puas sebesar 70% dan puas sebesar 30%.Kata kunci: Pelatihan, Soal, Penilaian Daring, google form
Pelatihan Teknis Penyusunan Media Pembelajaran Berbasis Video dengan Aplikasi Inshot pada Guru Biologi MGMP Biologi SMA di Kulon Progo
Authors: Rahmawati Yunita Fera , Prakarsa Tatag Bagus Putra , Harjana Tri , Ciptono Ciptono , Suhandoyo Suhandoyo 
Published: February 2024

Dosen Berkegiatan di Luar Kampus (DLK) ini ini bertujuan untuk meningkatkan efektivitas pembelajaran bagi guru MGMP Biologi SMA di Kulon Progo dalam memilih, merancang, melaksanakan media pembelajaran online untuk memanfaatkan teknologi informasi dan teknologi (TIK) seiring perkembangan Revolusi Industri 4.0. Adanya media pembelajaran yang tepat dapat menjadikan pembelajaran di kelas lebih efektif dan efisien. Pelatihan dilaksanakan dalam bentuk workshop secara tatap muka yang diikuti oleh guru-guru MGMP Biologi SMA di Kulon Progo pada tanggal 28 Mei-18 Agustus 2022 di Laboratorium micro teaching Jurusan Pendidikan Biologi FMIPA UNY dan SMAN 1 Pengasih, Kulon Progo. Materi pelatihan meliputi gambaran umum story board, pengenalan aplikasi inshot, praktik membuat video sederhana dengan bimbingan langsung dari tim DLK. Berdasarkan analisis pretest dan posttest terdapat peningkatan pengetahuan peserta terkait pengetahuan menggunakan inshot, pengenalan menu dan bagaimana menggunakannya dalam membuat video pembelajaran. Pelatihan ini dinilai cocok dengan kebutuhan peserta. Hal tersebut diketahui melalui kuisioner yang dibagikan pada akhir pelatihan. Sebanyak 81% peserta berharap dapat mengetahui cara mengoperasikan aplikasi inshot dengan baik agar dapat digunakan untuk pembelajaran di sekolah utamanya pada mata pelajaran Biologi. Sedangkan 19% peserta menyatakan ingin mengenal aplikasi inshot untuk membuat alternatif atau variasi metode pembelajaran di kelas agar peserta didik tidak bosan di kelas. Melalui pelatihan ini, harapannya dapat mempermudah proses belajar mengajar dan menarik perhatian peserta didik pada setiap pembelajaran Biologi.
PERS DAN PENYEBARAN BENIH NASIONALISME: FILANTROPI PENGUSAHA ROKOK KRETEK KUDUS UNTUK PERS BUMIPUTERA MASA KOLONIAL
Authors: Supratno Edy 
Published: February 2024

AbstrakSelain melalui berbagai buku bacaan yang diperoleh di dunia pendidikan, isu kebangsaan yang diusung kelompok nasionalis pada awal abad ke-20 juga banyak tersebar melalui pers atau media massa. Di saat yang sama, mengelola media massa membutuhkan modal yang kuat demi keberlangsungan operasional sebuah penerbitan. Industri rokok kretek di Kudus menjadi salah satu yang fenomenal di Hindia Belanda karena pada masa itu pengusaha Bumiputera berhasil mencapai posisi tertentu. Dari hasil usaha rokok kretek mereka menjadi kaya raya dan menyandang status social baru, salah satunya adalah Nitisemito. Penelitian ini menggunakan metode sejarah yang sebagian di antaranya menggunakan sumber koran yang terbit pada masanya. Tujuan dari penelitian ini untuk mengetahui bagaimana sumbangsih perusahaan rokok kretek Kudus dalam menghidupi media massa yang dikelola oleh Bumiputera pada masa-masa perkembangan nasionalisme di Indonesia. Kata kunci: Nasionalisme, Filantropi, Pers, Kretek, Kudus.
Scientific and Methodological Approach to Assessing Timeliness Operational Management of Vehicle Routing in an Urban Agglomeration
Authors: Belov A. S. , Reformat A. N. , Trakhinin E. L. , Birkun N.  I. 
Published: March 2024

Purpose of reseach. The purpose of this article is to develop a scientific and methodological approach to assessing the timeliness of decision-making in the operational management of car traffic routing in an urban agglomeration. Timeliness is manifested in reducing the time to prepare a decision to change the route of a car delivering goods in conditions of a possible (predicted) increase in traffic congestion at intersections and streets crossing the established route in an urban agglomeration. Methods. The presented approach is based on the basic principles of management theory in organizational systems, the theory of rational consumer behavior, mathematical statistics, and simulation modeling.Results. A variant of the generalized scheme of the operational control cycle for the routing of a car in an urban agglomeration has been developed. A sequence of estimation of decision-making time for operational control of vehicle traffic routing is proposed, and the individual stages of the evaluation sequence implement the main functions of the developed version of the operational control cycle. Experimental dependences of the time of analysis of route parameters during operational management on the quantity and quality of the routes under consideration in the AnyLogic 8.4.0 simulation environment are obtained.Conclusion. The article considers an approach to assessing the timeliness of decision-making in the operational management of the route of a car delivering goods in an urban agglomeration. To minimize the time for making an operational decision, it is proposed to predict the intensity of traffic congestion and assess the quality of the route. The approach allows, based on forecasting the state of the road situation at the intersection points of the route and streets with a high probability of traffic congestion, as well as, taking into account the number and quality of routes, to increase the timeliness of making a decision to change the route of a car delivering goods to an urban agglomeration.
Trapping Sets Search Using the Method of Mixed Integer Linear Programming with a Priori List of Variable Nodes
Authors: Usatjuk V S. , Egorov S. I. 
Published: March 2024

Purpose of researchis to develop a new high-speed method for searching trappin sets in graph codes, ensuring the completeness of the search.Methods. There are two approaches to finding trappin sets. The first, based on the Monte Carlo method with a biased probability estimation using Importance Sampling, involves the use of a decoder. The advantage of this approach is its high performance. The disadvantages are the dependence on decoder parameters and channel characteristics and the finite probability of missing trappin sets. The second approach is based on the use of linear programming methods. The advantage of this approach is the completeness of the resulting list of trappin sets, due to its independence from the decoder parameters and channel characteristics. The disadvantage of this approach is its high computational complexity. In the article, within the framework of the second approach, a new method for searching trappin sets with less computational complexity is proposed. The method involves solving a mixed integer linear programming problem using an a priori list of code vertices participating in the shortest cycles in the code graph.Results.Using the proposed method, a search for trappin sets was performed for several low-density codes. For this purpose, the mathematical linear programming package IBM CPLEX version 12.8 was used, which was run on 32 threads of a 16-core AMD Ryzen 3950X processor with 32GB of RAM (DDR4). In the Margulis code (2640, 1320), using the proposed method, the trappin set TS(6,6) was found in a time of 0.53 s. The speedup provided by the method proposed in the paper compared to the Velazquez-Subramani method is 8252.415 times. Thanks to the high speed and completeness of the search, trappin sets were found for the first time TS(62,16) and TS(52,14) in the Margulis code (4896, 2474 ).Conclusion.The paper proposes a new method for searching trapping sets by solving a mixed integer linear programming problem with an a priori list of code. The method is fast and provides completeness of the search.
Six-Month Outcomes of a Theory- and Technology-Enhanced Physical Activity Intervention for Latina Women (Pasos Hacia La Salud II): Randomized Controlled Trial
Authors: Connell Bohlen Lauren , Dunsiger Shira I , von Ash Tayla , Larsen Britta A , Pekmezi Dori , Marquez Becky , Benitez Tanya J , Mendoza-Vasconez Andrea , Hartman Sheri J , Williams David M , Marcus Bess H 
Published: March 2024

Background
More than half (55%) of Latina women do not meet aerobic physical activity (PA) guidelines, and frequently cite time, childcare, and transportation as barriers to PA. In addition to linguistic adaptations for this population, successful PA interventions for Latina women addressed these barriers through remote intervention delivery approaches (eg, mail, phone, or web delivery).

Objective
We aimed to evaluate 6-month outcomes of a randomized trial comparing a Spanish-language, individually tailored, web-delivered PA intervention (original) to an enhanced version with text messages and additional features (enhanced). Further, we evaluated if increases in PA at 6 months were moderated by baseline activity status.

Methods
In total, 195 Latina women aged 18-65 years participated in a trial comparing the efficacy of the enhanced versus original interventions at initiating PA behavior change. We examined minutes per week of accelerometer-measured PA in the enhanced versus original arms, and the proportion of each arm meeting aerobic PA guidelines (150 min/wk at 6 mo). For moderator analyses, participants were classified as inactive (0 min/wk) or low active (1-90 min/wk) at baseline, measured via the 7 Day Physical Activity Recall interview.

Results
PA increased from 19.7 (SD 47.9) minutes per week at baseline to 46.9 (SD 66.2) minutes per week at 6 months in the enhanced arm versus 20.6 (SD 42.7) minutes per week to 42.9 (SD 78.2) minutes per week in the original arm (P=.78). Overall, 30% (31/103) of the enhanced group met aerobic PA guidelines at 6 months, compared to 21% (19/92) of the original group (odds ratio [OR] 1.75, 95% CI 0.87-3.55). Baseline PA (inactive vs low active) moderated treatment effects on PA. For inactive participants, there were no group differences at 6 months (b=7.1; SE 22.8; P=.75), while low-active participants increased more in enhanced than original (b=72.5; SE 27.9; P=.01). For low-active participants, 45% (46/103) of the enhanced group met PA guidelines at 6 months, versus 20% (18/92) of the original arm (OR 3.29, 95% CI 1.05-11.31). For inactive participants, there were no group differences (25/103, 24% vs n=19/92, 21% for enhanced vs original, respectively; OR 1.28, 95% CI 0.54-3.06).

Conclusions
Intervention effects were conditional on baseline PA. For low-active Latina women, the enhanced intervention was more effective at increasing PA. Additional tailored intervention enhancements may be necessary to increase PA for inactive Latina women.

Trial Registration
ClinicalTrials.gov NCT03491592; https://www.clinicaltrials.gov/study/NCT03491592

International Registered Report Identifier (IRRID)
RR2-10.1186/s13063-022-06575-4
Moderating Effect of Depression on Glycemic Control in an eHealth Intervention Among Black Youth With Type 1 Diabetes: Findings From a Multicenter Randomized Controlled Trial
Authors: Ellis Deborah , Carcone April Idalski , Templin Thomas , Evans Meredyth , Weissberg-Benchell Jill , Buggs-Saxton Colleen , Boucher-Berry Claudia , Miller Jennifer L , Drossos Tina , Dekelbab M Bassem 
Published: February 2024

Background
Black adolescents with type 1 diabetes (T1D) are at increased risk for suboptimal diabetes health outcomes; however, evidence-based interventions for this population are lacking. Depression affects a high percentage of youth with T1D and increases the likelihood of health problems associated with diabetes.

Objective
Our aim was to test whether baseline levels of depression moderate the effects of a brief eHealth parenting intervention delivered to caregivers of young Black adolescents with T1D on youths’ glycemic control.

Methods
We conducted a multicenter randomized controlled trial at 7 pediatric diabetes clinics located in 2 large US cities. Participants (N=149) were allocated to either the intervention group or a standard medical care control group. Up to 3 intervention sessions were delivered on a tablet computer during diabetes clinic visits over a 12-month period.

Results
In a linear mixed effects regression model, planned contrasts did not show significant reductions in hemoglobin A1c (HbA1c) for intervention adolescents compared to controls. However, adolescents with higher baseline levels of depressive symptoms who received the intervention had significantly greater improvements in HbA1c levels at 6-month follow-up (0.94%; P=.01) and 18-month follow-up (1.42%; P=.002) than those with lower levels of depression. Within the intervention group, adolescents had a statistically significant reduction in HbA1c levels from baseline at 6-month and 18-month follow-up.

Conclusions
A brief, culturally tailored eHealth parenting intervention improved health outcomes among Black adolescents with T1D and depressive symptoms.

Trial Registration
ClinicalTrials.gov NCT03168867; https://clinicaltrials.gov/study/NCT03168867
Combined Serologic and Genetic Risk Score and Prognostication of Phospholipase A2 receptor-Associated Membranous Nephropathy
Authors: Hu Xiaofan , Xu Jing , Wang Wei , Liu Lili , Jing Yuanmeng , Gao Chenni , Yu Xialian , Li Yi , Lin Li , Tong Jun , Weng Qinjie , Pan Xiaoxia , Zhang Wen , Ren Hong , Li Guisen , Kiryluk Krzysztof , Chen Nan , Xie Jingyuan 
Published: March 2024

Introduction
The aim of this study was to test whether a combined risk score on the basis of genetic risk and serology can improve the prediction of kidney failure in phospholipase A2 receptor (PLA2R)-associated primary membranous nephropathy.

Methods
We performed a retrospective analysis of 519 biopsy-proven PLA2R-associated primary membranous nephropathy patients with baseline eGFR ≥25 ml/min per 1.73 m2. The combined risk score was calculated by combining the genetic risk score with PLA2R ELISA antibody titers. The primary end point was kidney disease progression defined as a 50% reduction in eGFR or kidney failure. Cox proportional hazard regression analysis and C-statistics were applied to compare the performance of PLA2R antibody, genetic risk score, and combined risk score, as compared with clinical factors alone, in predicting primary outcomes.

Results
The median age was 56 years (range, 15–82 years); the male-to-female ratio was 1:0.6, the median eGFR at biopsy was 99 ml/min per 1.73 m2 (range: 26–167 ml/min per 1.73 m2), and the median proteinuria was 5.3 g/24 hours (range: 1.5–25.8 g/24 hours). During a median follow-up of 67 (5–200) months, 66 (13%) had kidney disease progression. In Cox proportional hazard regression models, PLA2R antibody titers, genetic risk score, and combined risk score were all individually associated with kidney disease progression with and without adjustments for age, sex, proteinuria, eGFR, and tubulointerstitial lesions. The best-performing clinical model to predict kidney disease progression included age, eGFR, proteinuria, serum albumin, diabetes, and tubulointerstitial lesions (C-statistic 0.76 [0.69–0.82], adjusted R2 0.51). Although the addition of PLA2R antibody titer improved the performance of this model (C-statistic: 0.78 [0.72–0.84], adjusted R2 0.61), replacing PLA2R antibody with the combined risk score improved the model further (C-statistic: 0.82 [0.77–0.87], adjusted R2 0.69, difference of C-statistics with clinical model=0.06 [0.03–0.10], P < 0.001; difference of C-statistics with clinical–serologic model=0.04 [0.01–0.06], P < 0.001).

Conclusions
In patients with PLA2R-associated membranous nephropathy, the combined risk score incorporating inherited risk alleles and PLA2R antibody enhanced the prediction of kidney disease progression compared with PLA2R serology and clinical factors alone.
The Impact of Macroeconomic Fundamental Policies on The Composite Stock Price Index
Authors: Nadhilla Zhafa , Sriyono Sriyono 
Published: February 2024

This study aims to test the purpose of this study, namely to determine the effect of Gross Domestic Product, Inflation, Interest Rates and Rupiah Exchange Rate on the Composite Stock Price Index.This type of research uses quantitative methods and using secondary data. The data used in this study are time series of Gross Domestic Product, Inflation, Interest Rates, Rupiah Exchange Rate and JCI during the 2011-2020 period. Sampling from this study used the saturated sample method from the monthly data of each variable for 10 years, so that 120 samples were obtained. This research uses multiple linear analysis techniques with the application of eviews 9. The results of this study show that the Gross Domestic Product variable has a positive and significant influence on the JCI, the Inflation variable has a positive and significant influence on the JCI, the Interest Rate variable does not have a significant influence on the JCI and the Rupiah Exchange Rate variable has a negative and significant influence on the JCI.
Vildagliptin Nephroprotective Effect in Rats Model with Cisplatin-Induced Nephrotoxicity
Authors: Watife Abeer T. , Bairam Ahsan F. , Al-Ghuraibawi Nibras H. 
Published: February 2024

Objective: To evaluate the nephroprotective effect of vildagliptin against cisplatin-induced nephrotoxicity in rats.
Methods and Materials: Twenty-eight male rats have been divided into four groups: Control (received distal water), cisplatin treated group (received single dose of cisplatin (7mg/kg) intraperitoneally (IP) on day eight), vildagliptin plus cisplatin treated group (received vildagliptin 10mg/kg/day orally for 14 days, seven days before and seven days after the dose of cisplatin on day eight), and vildagliptin treated group (received the same dose and duration of vildagliptin mentioned previously). At the end, blood samples were collected to evaluate tumor necrotic factor-α (TNF-α), caspase-3, total antioxidant capacity (TAOC), urea, and creatinine. The serum levels of these biomarkers were expressed as mean ± standard error of mean. Additionally, kidneys were fixed in formalin for histopathological examination.
Result: Vildagliptin treatment significantly reduced the serum levels of TNF-α, caspase-3, urea, and creatinine as well as increased the TAOC level in rats treated with vildagliptin plus cisplatin when compared with cisplatin treated group. Histopathological examination further supported the nephroprotective effect of vildagliptin in rats with cisplatin induced nephrotoxicity.
Conclusion: Vildagliptin improved kidney function and reduced cisplatin nephrotoxicity which may highlight the nephroprotective effect of this DPP-4 inhibitor against cisplatin-induced nephrotoxicity.