bluuebunny/crossref_metadata_2025_split huggingface dataset:
A key predistribution scheme for deployable sensor networks using the node deployment probability density function
Authors: Ito Takashi , Ohta Hidenori , Matsuda Nori , Yoneda Takeshi 
Published: September 2007

AbstractIn order to establish encrypted communications in deployable sensor networks, it is essential that encryption keys be shared between nodes. However, the sharing of keys is not simple given the restricted resources of such devices. In recent years, several random key predistribution schemes have been proposed; in the scheme proposed by Du and colleagues, improved performance is achieved by using information regarding the deployment location when predistributing keys. However, this scheme suffers from the problem that it is possible to apply only for the so‐called group deployment model whereby nodes are partitioned into groups and deployed from locations that are arranged systematically. In this paper we propose a random key predistribution scheme that can be applied to arbitrary deployment models by making use of probability distribution information regarding the deployment when the key predistribution is performed. In addition, we confirm from computational experiments that this method is able to construct networks that are more consolidated than those created by Du and colleagues' scheme. © 2007 Wiley Periodicals, Inc. Electron Comm Jpn Pt 2, 90(10): 73–83, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjb.20390
System for judgment of depressive tendency from speech analysis
Authors: Suzuki Tomoko , Tamura Naoyoshi 
Published: September 2007

AbstractThe objective of this investigation was to construct a system for judgment of depressive tendency. A sentence reading experiment was performed with 15 subjects with a highly depressive tendency and 15 subjects with a mildly depressive tendency selected using the BDI, a measure of depressive tendency. The spoken sentences were fed to a computer, and the amplitude, fundamental frequency, and utterance speed were calculated. Statistical tests were applied to each physical feature parameter of the speech, and the relation to the degree of depressive tendency of the subject in the sentence reading was determined. The objective was to identify index words that well express the depressive tendency. For three index word selection strategies, the important physical parameters of speech were extracted by logistic multiple regression analysis. The experiment and analysis indicated that the statistical index word selection strategy was more effective in judgment than the other two index word selection strategies. © 2007 Wiley Periodicals, Inc. Electron Comm Jpn Pt 2, 90(10): 103–114, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjb.20392
Pedestrian detection with a vehicle camera using fast template matching based on background elimination and active search
Authors: Mochizuki Daisuke , Yano Yoshikazu , Hashiyama Tomonori , Okuma Shigeru 
Published: September 2007

AbstractTemplate matching using the silhouette shapes of pedestrians as features is widely used as a method for detecting pedestrians in images taken from an in‐vehicle camera. Because of the need to detect a variety of pedestrian shapes, the template matching involves scanning the entire image for a very large number of templates. A large number of computations are needed to utilize multiple templates, and so it is difficult to implement the processing at the video frame rate. Furthermore, because images of outdoor street scenes that are captured by an in‐vehicle camera consist of problematic backgrounds, false detections occur easily in the background area. A method is therefore proposed to perform highly efficient, high‐speed matching by removing the background region, as this region has a negative impact on the detection capabilities in template matching. The background removal is achieved by using difference calculations that use information about the motion of the observation measured by sensors. Furthermore, an active search is carried out on the images after removing the background to give an efficient search, and a broad reduction in the number of computations is achieved. © 2007 Wiley Periodicals, Inc. Electron Comm Jpn Pt 2, 90(10): 115–126, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/ecjb.20393
Divalent Nickel, Cobalt and Iron Complexes of an Amide‐Appended N2S2 Ligand: Synthesis, Characterization and Reactivity with Hydroxide Anion
Authors: Ingle Gajendrasingh K. , Makowska‐Grzyka Magdalena M. , Arif Atta M. , Berreau Lisa M. 
Published: September 2007

AbstractDivalent nickel, cobalt and iron complexes {[(bmppa)Ni(CH3CN)](ClO4)2 (1), [(bmppa)Co(CH3CN)](ClO4)2 (2), [(bmppa)Fe(CH3CN)](ClO4)2 (3)} of an amide‐appended N2S2‐donor ligand {bmppa = N,N‐bis(2‐methylthio)ethyl‐N‐[(6‐pivaloylamido‐2‐pyridyl)methyl]amine} have been prepared and characterized using 1H NMR, IR, UV/Vis, elemental analysis and magnetic moment measurements. The complexes 2 and 3 were also characterized by X‐ray crystallography. When treated with 1 equiv. of Me4NOH·5H2O in methanol, 1 and 2 form deprotonated amide complexes {[(bmppa–)Ni]ClO4 (4), [(bmppa–)Co]ClO4·H2O (5)} which were isolated and characterized by 1H NMR, IR, UV/Vis, elemental analysis and magnetic moment measurements. Treatment of the FeII complex 3 with 1 equiv. of Me4NOH·5H2O in methanol produced the free bmppa chelate ligand and a precipitate of an unidentified iron species. Heating of 5 at 50 °C in methanol for 5 d resulted in a ca. 50 % yield of amide methanolysis products and a small amount of products resulting from oxidative N‐dealkylation of the bmppa ligand. It has been previously shown that a zinc analog of 5 undergoes quantitative amide methanolysis under milder conditions. Complex 4 does not undergo amide cleavage upon heating at 50 °C in methanol for 5 d. A rationale for the metal‐dependent amide methanolysis reactivity of these complexes is proposed on the basis of structural differences in the “parent” protonated complexes. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
The Influence of Ligand Substitution at the Electron Donor Center in Molecular Cyano‐Bridged Mixed‐Valent CoIII/FeII and CoIII/RuII Complexes
Authors: Bernhardt Paul V. , Bozoglian Fernando , Font‐Bardía Mercè , Martínez Manuel , Meacham Andrew P. , Sienra Beatriz , Solans Xavier 
Published: September 2007

AbstractIn this paper we report a new variety of dinuclear cyano‐bridged CoIII/MII (M = Fe or Ru) mixed‐valent complexes of the general formula [LnCoIII(μ‐NC)MII(CN)x(bpy)y]z+ (where Ln represents a pentaamine macrocycle and M = Fe or Ru with x = 4, y = 1 and z = 1 or x = 2, y = 2 and z = 3)]. The incorporation of the bpy ligand in place of cyano ligands within the coordination sphere raises the MIII/II redox potential significantly relative to the hexacyanometalate analogs we have reported previously. However, the metal‐to‐metal (MII to CoIII) charge‐transfer transitions are shifted to lower energies in accordance with Hush theory as a result of a decreased reorganizational energy component, which result in them overlapping with more intense MLCT (MII to bpy) transitions. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Solid/liquid extraction and isolation by molecular distillation of hydroxytyrosol from Olea europaea L. leaves
Authors: Rada Mirela , Guinda Ángeles , Cayuela Jose 
Published: September 2007

AbstractMolecular distillation, or short‐path distillation (SPD), is particularly appropriate for processing of low‐volatility compounds, which are easily altered at high temperature. Olea europaea L. leaves constitute an olive tree by‐product very interesting for their natural antioxidants content. In this research, molecular distillation technology has been applied to obtain high‐value‐added compounds by the SPD fractionation of an olive tree leaf extract. The process consists of two stages: (a) ethanolic extraction of the olive leaves, followed by incorporation of the extract into glycerine and (b) molecular distillation of the glycerine enriched in olive leaf extract compounds (terpenic and phenolic compounds). Four molecular distillation tests under different conditions were carried out. Results showed that 80.9% 3,4‐dihydroxy‐phenylethanol (hydroxytyrosol) was recovered from the glycerine admixture under a pressure of 1.50–2.00 mbar, a temperature of 190 °C and a feed rate of 15 mL/min.
The Tautomerism of 5‐Amino‐3‐oxo‐1,2,4‐thiadiazole: An Experimental and Theoretical Study
Authors: Encinas Arantxa , Castro Ana , Campillo Nuria E. , Páez Juan A. 
Published: September 2007

AbstractThe 1,2,4‐thiadiazole system was the subject of our research as a consequence of the pharmacological activity of some derivatives as GSK3 inhibitors. Therefore, in order to explore the active form responsible for receptor interaction, a systematic study of the tautomerism in the 5‐amino‐3‐oxo‐1,2,4‐thiadiazole system was performed by using experimental and theoretical methods. Thus, the relative stability of the possible tautomers was studied in the gas phase by density functional theory and local density functional methods. The theoretical study in solution was carried out by using severalcontinuum solvation models. Finally, experimental studies were carried out to unambiguously establish the tautomeric form.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Downregulation of the 18‐kDa translocator protein: Effects on the ammonia‐induced mitochondrial permeability transition and cell swelling in cultured astrocytes
Authors: Panickar K. S. , Jayakumar A. R. , Rama Rao K. V. , Norenberg M. D. 
Published: September 2007

AbstractHepatic encephalopathy (HE) is a major neurological complication in patients with severe liver disease. While the pathogenesis of HE is unclear, elevated blood and brain ammonia levels are believed to be major etiological factors, and astrocytes appear to be the primary target of its toxicity. A notable feature of ammonia neurotoxicity is an upregulation of the 18‐kDa translocator protein (TSPO) (formerly referred to as the peripheral benzodiazepine receptor or PBR), which is found on the outer mitochondrial membrane. However, the precise significance of this upregulation is unclear. To examine its potential role in ammonia‐induced astrocyte dysfunction, we downregulated the TSPO using antisense oligonucleotides, and examined whether such downregulation could alter two prominent features of ammonia gliotoxicity, namely, the mitochondrial permeability transition (MPT) and astrocyte swelling. Nontransfected cultures treated with NH4Cl (5 mM; 48 h) showed a significant increase in astrocyte cell volume (37.5%). In cultured astrocytes transfected with TSPO antisense oligonucleotides, such cell swelling was reduced to 17%, but this change was not significantly different from control cell volume. Similarly, nontransfected cultures treated with NH4Cl (5 mM; 24 h) exhibited a 40% decline in the cyclosporin A‐sensitive mitochondrial inner membrane potential (ΔΨm) (P< 0.01) (a measure of the MPT). By contrast, cells transfected with TSPO antisense oligonucleotides did not display a significant loss of the ΔΨmfollowing ammonia exposure. Our findings highlight the important role of the TSPO in the mechanism of ammonia neurotoxicity. © 2007 Wiley‐Liss, Inc.
Hydroclimate controls over seasonal sediment yield in two adjacent High Arctic watersheds
Authors: Cockburn Jaclyn M. H. , Lamoureux Scott F. 
Published: September 2007

AbstractInterannual variations in seasonal sediment transfer in two High Arctic non‐glacial watersheds were evaluated through three summers of field observations (2003–2005). Total seasonal discharge, controlled by initial watershed snow water equivalence (SWE) was the most important factor in total seasonal suspended sediment transfer. Secondary factors included melt energy, snow distribution and sediment supply. The largest pre‐melt SWE of the three years studied (2004) generated the largest seasonal runoff and disproportionately greater suspended sediment yield than the other years. In contrast, 2003 and 2005 had similar SWE and total runoff, but reduced runoff intensity resulted in lower suspended sediment concentrations and lower total suspended sediment yield in 2005. Lower air temperatures at the beginning of the snowmelt period in 2003 prolonged the melt period and increased meltwater storage within the snowpack. Subsequently, peak discharge and instantaneous suspended sediment concentrations were more intense than in the otherwise warmer 2005 season. The results for this study will aid in model development for sediment yield estimation from cold regions and will contribute to the interpretation of paleoenvironmental records obtained from sedimentary deposits in lakes. Copyright © 2007 John Wiley & Sons, Ltd.
Characterization of erythropoietin receptor and erythropoietin expression and function in human ovarian cancer cells
Authors: Jeong Jee‐Yeong , Feldman Laurie , Solar Peter , Szenajch Jolanta , Sytkowski Arthur J. 
Published: September 2007

AbstractThe identification of erythropoietin receptors (EpoR) on cancer cells has caused concern, since it implies the possibility that treatment of cancer patients with erythropoietin (Epo) and related agents with demonstrable antiapoptotic activity could enhance cancer growth and progression. However, the function and even the validity of the identification of these receptors have been called into question. We now report the characterization of EpoR and Epo expression by 4 human ovarian cancer cell lines: A2780, CaOV, SKOV and OVCAR‐3. Using semiquantitative RT‐PCR, restriction digestion of the PCR products and DNA sequence analysis, we determined that each of the lines expresses the EpoR and Epo at the mRNA level. A2780 cells were the highest expressers of both genes. We demonstrated EpoR protein both by western blotting and by immunofluorescence and biologically active Epo protein by quantitative in vitro bioassay. The EpoR on A2780 cells was shown to be functional, since Epo stimulation resulted in phosphorylation of Erk1/2, an important EpoR mitogenic signaling intermediate. None of the cell lines exhibited a growth response in culture to exogenous Epo. However, addition of a neutralizing anti‐Epo antibody to A2780 cells resulted in partial growth inhibition that was reversed by the addition of excess Epo, providing evidence for an autocrine/paracrine mechanism of growth enhancement in these cells. © 2007 Wiley‐Liss, Inc.
Erythropoietin treatment of human ovarian cancer cells results in enhanced signaling and a paclitaxel‐resistant phenotype
Authors: Solar Peter , Feldman Laurie , Jeong Jee‐Yeong , Busingye Jacqueline R. , Sytkowski Arthur J. 
Published: September 2007

AbstractErythropoietin (Epo), a glycoprotein hormone that is the principal regulator of erythropoiesis, is known to act also on nonhematopoietic cell types. Epo receptors have been reported on several normal and neoplastic human cells and tissues, including ovarian cancer cells. We found that long‐term Epo treatment of A2780 cells resulted in the development of a phenotype exhibiting both enhanced Epo signaling, evidenced by increased peak levels of phospho‐Erk1/2 and increased paclitaxel resistance. This phenotypic effect was specific for paclitaxel, since no change in cisplatin or carboplatin sensitivity was observed. In addition, the change in phenotype was stable, even after the removal of Epo. Measurement of mono‐ and oligonucleosome formation revealed that long‐term Epo treated A2780 cells exhibited markedly less apoptosis than nonerythropoietin treated cells at essentially all concentrations of paclitaxel tested. Western blot analyses revealed that the long‐term Epo treated cells had significantly reduced expression of apoptosis‐related proteins Bcl‐2 and Bcl‐10. These findings may have implications for the clinical use of recombinant human Epo and other erythropoiesis stimulating agents to correct anemia in paclitaxel‐treated cancer patients. © 2007 Wiley‐Liss, Inc.
The CD155/poliovirus receptor enhances the proliferation of ras‐mutated cells
Authors: Kono Tokuyuki , Imai Yasuo , Yasuda Shin‐ichi , Ohmori Kyoko , Fukui Hirokazu , Ichikawa Kazuhito , Tomita Shigeki , Imura Johji , Kuroda Yoshikazu , Ueda Yoshihiko , Fujimori Takahiro 
Published: September 2007

AbstractStimulation of the CD155/poliovirus receptor, which localizes in the cell–matrix and at cell–cell junctions, inhibits cell adhesion and enhances cell migration. Necl‐5, a mouse homolog of CD155, is implicated in the formation of adherence junctions. Recently, Necl‐5 has also been found to enhance cell proliferation via the stimulation of serum and platelet‐derived growth factor through the Ras‐Raf‐MEK‐ERK signaling pathway. In our present study, we find that CD155 significantly enhances the serum‐induced cell proliferation of NIH3T3 cells which have been transformed by an oncogenic Ras (V12Ras‐NIH3T3), but not the parental cells. CD155 expression in V12Ras‐NIH3T3 cells is also found to upregulate cyclin D2, downregulate p27Kip1 and shorten the G0/G1 phase of the cell cycle. An inhibitor of focal adhesion kinase does not reduce this CD155‐mediated enhancement of V12Ras‐NIH3T3 cell proliferation. The expression of CD155ΔCP, which lacks the cytoplasmic region including the immunoreceptor tyrosine‐based inhibitory motif (ITIM), has a reduced ability to enhance the serum responsiveness of V12Ras‐NIH3T3 cells, suggesting that the ITIM might be required for this effect of CD155. In addition, the overexpression of exogenous CD155 enhances the serum responsiveness of HT1080 cells, which harbor a mutant N‐ras gene. On the other hand, siRNA‐induced knockdown of endogenous CD155 and/or CD155ΔCP expression significantly repress the serum responsiveness of DLD‐1 cells, which express endogenous CD155 and harbor a mutant K‐ras gene, suggesting that this mutant may function in a dominant negative manner. Taken together, our present data suggest that CD155, at least in part, enhances the proliferation of ras‐mutated cells. © 2007 Wiley‐Liss, Inc.
Involvement of SDF‐1α/CXCR4 axis in the enhanced peritoneal metastasis of epithelial ovarian carcinoma
Authors: Kajiyama Hiroaki , Shibata Kiyosumi , Terauchi Mikio , Ino Kazuhiko , Nawa Akihiro , Kikkawa Fumitaka 
Published: September 2007

AbstractEpithelial ovarian carcinoma (EOC) spreads by implantation of tumor cells onto the human peritoneal mesothelial cells (HPMCs) lining the peritoneal cavity. The aim of this study was to determine whether the stromal cell‐derived factor‐1α (SDF‐1α)/CXCR4 axis is involved in the interaction of EOC cells with HPMCs in peritoneal metastasis. Clinically, we first evaluated CXCR4 expression in sections from 36 primary EOCs using immunohistochemistry. We next examined whether SDF‐1α played roles in EOC progression, including in proliferation, cell motility, attachment to HPMCs, and the in vivo development of peritoneal metastasis through CXCR4. Of the 36 carcinomas, 16 cases (44.4%) were positive for CXCR4 immunoexpression. Positive CXCR4 expression significantly predicted poorer overall survival compared with negative expression (p = 0.0069). We found CXCR4 expression in both EOC cells and HPMCs. In contrast, the level of production of SDF‐1α by HPMCs was higher than that by various EOC cells. Functionally, SDF‐1α induced enhanced attachment between ES‐2 cells and HPMCs or extracellular matrix components. The enhancement of adhesion potential by SDF‐1α was inhibited by AMD3100, a CXCR4 antagonist, and by phosphatidylinositol 3 kinase and p44/42 inhibitors. Furthermore, intraperitoneal treatment with AMD3100 resulted in reduced dissemination in nude mice inoculated with ES‐2 cells. The present results suggest that there may be a link between the SDF‐1α/CXCR4 axis and enhanced intraperitoneal dissemination of EOC and that CXCR4 may be a novel target for the treatment of EOC. © 2007 Wiley‐Liss, Inc.
Human papillomavirus (HPV) genotype distribution in invasive cervical cancers in France: EDITH study
Authors: Prétet Jean‐Luc , Jacquard Anne‐Carole , Carcopino Xavier , Charlot Jean‐François , Bouhour Damien , Kantelip Bernadette , Soubeyrand Benoit , Leocmach Yann , Mougin Christiane , Riethmuller Didier ,   for the EDITH study group
Published: September 2007

AbstractInvasive cervical cancer (ICC) remains a significant cause of morbidity and mortality in France. Since human papillomavirus (HPV) is the necessary cause of ICC, the aim of this study was to assess the type‐specific prevalence of HPV in ICC in France in order to locally evaluate the potential benefit of an HPV 16/18 L1 virus‐like particles (VLP) vaccination. A total of 516 histological specimens collected in 15 centers were analyzed. Among them, 86% had a diagnosis of squamous cell carcinoma (SCC) whereas 14% were adenocarcinomas (ADC). HPV genotyping was performed using the INNO‐LiPA assay allowing the specific detection of 24 HPV genotypes both high risk (HR) and low risk (LR). The overall HPV prevalence in ICC was 97%. The most prevalent genotypes were HPV 16 (73%) and HPV 18 (19%) followed by HPV 31 (7%), 33, 68, 45, 52 and 58 (4.1–2.3%). HPV 16 and/or 18 were associated with 82% of ICC, 10% being HPV 16 and 18 coinfections. While HPV 16 was the most prevalent type in both SCC (74%) and ADC (64%), HPV 18 was by far more prevalent in ADC (37%) compared to SCC (16%; p < 0.001). Multiple infections with at least two different HR HPV genotypes were observed in 22% of ICC. Given the high HPV 16/18 prevalence and taking into account possible production of crossneutralizing antibodies against other HPV types, HPV 16/18 L1 VLP vaccination would be expected to significantly reduce the burden of ICC in France. © 2007 Wiley‐Liss, Inc.
Human papillomavirus genotype distribution in high grade cervical lesions (CIN 2/3) in France: EDITH study
Authors: Prétet Jean‐Luc , Jacquard Anne‐Carole , Carcopino Xavier , Monnier‐Benoit Sylvain , Averous Gerlinde , Soubeyrand Benoit , Leocmach Yann , Mougin Christiane , Riethmuller Didier ,   for the EDITH study group
Published: September 2007

AbstractHigh grade cervical intraepithelial neoplasia (CIN 2/3) have a high potential to progress to invasive cervical cancer (ICC). Pap testing including follow‐up and treatment of CIN 2/3 is currently the best prevention of ICC, but is associated with morbidity, namely obstetrical adverse effects and psychological distress. Human papillomavirus (HPV) is universally accepted as the necessary cause of ICC. The objective of the present study was to describe the type‐specific prevalence of HPV in CIN 2/3 in France and hereby to locally estimate the potential benefit of an HPV 16/18 L1 virus‐like particles (VLP) vaccine. A total of 493 formalin‐fixed and paraffin‐embedded CIN 2/3 specimens were analyzed. Medical records were examined for patient related data. HPV were genotyped with the INNO‐LiPA assay allowing the detection of 24 HPV genotypes. The overall prevalence of LiPA detectable HPV was 98%. The most prevalent genotype was HPV 16 (62%) followed by HPV 31 (15%), 33 (12%), 52 (9%), 51 (8%), 58 (7%), 35 and 18 (4%). Multiple infection with at least two different high‐risk (HR) HPV genotypes was observed in 26% of all specimens including 2.6% with HPV 16 and 18 multiple infections. The present study indicates that HPV 16 is by far the most common HPV type associated with CIN 2/3 in France. With an HPV 16 and 18 prevalence of 64%, HPV 16/18 L1 VLP vaccines would be expected to significantly reduce the burden associated with the management and treatment of CIN 2/3 in France. © 2007 Wiley‐Liss, Inc.