bluuebunny/crossref_metadata_2025_split huggingface dataset:
Elucidation of the molecular mechanism of the breakage-fusion-bridge (BFB) cycle using a CRISPR-dCas9 cellular model
Authors: Singh Manrose , Raseley Kaitlin , Perez Alexis M. , MacKenzie Danny , Kosiyatrakul Settapong T , Desai Sanket , Batista Noelle , Guru Navjot , Loomba Katherine K. , Abid Heba Z. , Wang Yilin , Udo-Bellner Lars , Stout Randy F. , Schildkraut Carl L. , Xiao Ming , Zhang Dong 
Published: April 2024

AbstractChromosome instability (CIN) is frequently observed in many tumors. The breakage-fusion-bridge (BFB) cycle has been proposed to be one of the main drivers of CIN during tumorigenesis and tumor evolution. However, the detailed mechanisms for the individual steps of the BFB cycle warrants further investigation. Here, we demonstrated that a nuclease-dead Cas9 (dCas9) coupled with a telomere-specific single-guide RNA (sgTelo) can be used to model the BFB cycle. First, we showed that targeting dCas9 to telomeres using sgTelo impeded DNA replication at telomeres and induced a pronounced increase of replication stress and DNA damage. Using Single-Molecule Telomere Assay via Optical Mapping (SMTA-OM), we investigated the genome-wide features of telomeres in the dCas9/sgTelo cells and observed a dramatic increase of chromosome end fusions, including fusion/ITS+ and fusion/ITS-.Consistently, we also observed an increase in the formation of dicentric chromosomes, anaphase bridges, and intercellular telomeric chromosome bridges (ITCBs). Utilizing the dCas9/sgTelo system, we uncovered many novel molecular and structural features of the ITCB and demonstrated that multiple DNA repair pathways are implicated in the formation of ITCBs. Our studies shed new light on the molecular mechanisms of the BFB cycle, which will advance our understanding of tumorigenesis, tumor evolution, and drug resistance.
An iron-sulfur cluster as a new metal centre in a flavodiiron protein
Authors: Martins Maria C. , Silveira Célia M. , Morgado Leonor , Teixeira Miguel , Folgosa Filipe 
Published: April 2024

AbstractSyntrophomonas wolfeicontains two distinct multiple domain flavodiiron proteins (FDPs), of Classes H and E, presumably acting as oxygen reductases to protect this anaerobic bacterium from oxidative stress due to exposure to environments containing, even if only transiently, oxygen. The Class E FDP was predicted to have, besides the two core domains characteristic of this type of enzymes, an extra C- terminal domain putatively harbouring an iron-sulfur centre. Bioinformatic analyses showed that, thus far, Class E FDPs are only present in three other bacteria of theSyntrophomonasgenus:Syntrophomonas palmitatica,Syntrophomonas zenhnderiandThermosyntropha lipolytica.In this work, we extensively characterized the enzyme fromSyntrophomonas wolfei(wild type, site directed mutants and truncated domains) and showed unequivocally, using EPR and Resonance Raman spectroscopies, that indeed it contains a [3Fe- 4S]1+/0centre, a novelty in the field of FDPs. Structure prediction using Alphafold indicated some similarities to [3Fe4S]1+/0containing ferredoxins. The reduction potentials of each cofactor were determined: +70 mV, -5/-70 mV and -90 mV for the FeS, diiron centre and flavin, respectively.
The Structure of the Drp1 Lattice on Membrane
Authors: Peng Ruizhi , Rochon Kristy , Hutson Anelise N. , Stagg Scott M. , Mears Jason A. 
Published: April 2024

ABSTRACTMitochondrial health relies on the membrane fission mediated by dynamin-related protein 1 (Drp1). Previous structural studies of Drp1 on remodeled membranes were hampered by heterogeneity, leaving a critical gap in the understanding of the mitochondrial fission mechanisms. Here we present a cryo-electron microscopy structure of full-length human Drp1 decorated on membrane tubules. Using the reconstruction of average subtracted tubular regions (RASTR) technique, we report that Drp1 forms a locally ordered lattice along the tubule without global helical symmetry. The filaments in the lattice are similar to dynamin rungs with conserved stalk interactions. Adjacent filaments are connected by GTPase domain interactions in a novel stacked conformation. We identified two states of the Drp1 lattice among the heterogenous dataset representing conformational changes around hinge 1. Additionally, we observed contact between Drp1 and membrane that can be assigned to the variable domain sequence. Together these structures revealed a putative mechanism by which Drp1 constricts mitochondria membranes in a stepwise, “ratchet” manner.SUMMARYThis study provides new insights into the structure of Drp1 on lipid membranes. A locally ordered Drp1 lattice structure is solved and reveals intermolecular contacts and conformational rearrangements that suggest a mechanism for constriction of mitochondrial membranes.
Contextualizing work-family experiences in India: sex, gender role perception and urbanization
Authors: Raina Mahima , Cho Eunae 
Published: April 2024

PurposeDespite the recognition that contextual factors play a key role in shaping individuals’ work-family (WF) interface, empirical research that simultaneously considers individual, roles and contextual factors is scarce. Drawing on the pyramid model of work-home interface, we delve into the intersection among sex, gender role ideology (GRI) and urbanization (URB) in relation to WF conflict and enrichment in India. Specifically, we explored whether and how sex (male vs female), GRI (traditional vs egalitarian) and URB (big vs small city) interact to predict WF conflict and WF enrichment.Design/methodology/approachThe data were collected from 586 full-time employees working in both more and less urbanized cities in India. Moderation analyses were utilized to study the interaction effects on WF conflict and enrichment.FindingsResults indicate that GRI is a stronger driver of WF experiences, especially WF enrichment, for women regardless of location. The study contributes to the understanding of WF experiences in India and addresses the complexity of WF experiences, especially with respect to sex and gender.Originality/valueOur study offers a nuanced understanding of WF experiences in India by integrating micro- to macro-level antecedents, thereby addressing the complexity of WF experiences. While a lot of research explains sex and gender differences in WF experiences, our study highlights how these experiences vary with the degree of URB.
Dynamic analysis of a tensegrity-based traction structure
Authors: Liu Heping , Lu Jinxin , Zhu Fusheng , Luo Ani 
Published: April 2024

Purpose This study proposes a tensegrity-based traction structure with D-bar dual cable units. It is used to connect the airship and the ground to stabilize the airship.Design/methodology/approach The mathematical models and dynamic models of the D-bar dual cable (hereafter referred to as DD cable) unit of the tensegrity-based traction structure are established. Based on the minimum mass method, the mass of the DD cable unit in the critical state (cable member is yielding, or bar member is buckling or yielding) is analyzed. Then, the tensile strength of the DD cable unit and single cable unit under the same condition is compared using the control variate method. Finally, based on ANSYS dynamic simulation, the stability of the two structures under the same external force disturbance was tested.Findings Expressions for the minimum mass of the DD cable unit under different failure conditions are solved. Dynamic simulation results show that the capacity of resisting disturbance of the DD cable unit is much better than that of the single cable unit under the same wind speed. So, we find a structure more suitable for the fixed connection of an airship.Originality/value This study helps to provide theoretical reference and thinking for the practical application of the traction structure with a D-bar dual cable unit.
When social enterprise ecosystem shapes national public policy: examining Brazil’s national impact economy strategy
Authors: Mathias Ana Luiza Terra Costa , Souza Aline Gonçalves Videira de , Ribeiro Matheus de Mello Sá Carvalho 
Published: April 2024

PurposeSocial enterprises are embedded in ecosystems with multiple actors interested in the field’s growth. One way to enhance social enterprises is through public policies and developing countries like Brazil included this in the public agenda. After an important mobilization of private organizations and public managers, the Brazilian federal government implemented in 2017 the National Impact Investment and Business Strategy (ENIMPACTO) renamed in 2023 to National Impact Economy Strategy with the same abbreviation. Since its creation, ENIMPACTO saw significant modifications leading to a decree in 2023 extending its mandate, amplifying membership and changing its name to the National Impact Economy Strategy while maintaining the same acronym. This experience leads us to the following question: How was ENIMPACTO created and developed?Design/methodology/approachWe used institutional arrangements and advocacy coalition theory to analyze the key elements that contributed to ENIMPACTO’s creation and its evolution through time. A qualitative, single-case study on the Brazilian experience implementing ENIMPACTO was conducted through semi-structured interviews with national strategy members, participant observation, document and data analysis.FindingsWe argue that advocacy coalition and institutional arrangements frameworks combined are needed to understand Enimpacto’s complexity. The strategy presented an extensive multiple-actor articulation involving shared beliefs that were also important to gather support on recreating and expanding Enimpacto when external events threatened its continuity. Yet, it presented important challenges on how to achieve consensus and alignment regarding important concepts and regulation strategy among the actors and manage the public policy governance and activities implementation.Originality/valueWe combine institutional arrangements and advocacy coalition frameworks and apply them to analyze a public policy composed of actors of multiple sectors that play an active advocacy coalition role. We also present empirical evidence that elements of the advocacy coalition framework add analytical elements to institutional arrangements literature and how they affect each other. We point to two important elements of the institutional arrangements framework (territoriality and subsidiarity) that were not initially considered by ENIMPACTO and were later incorporated because of tensions in the field. We provide empirical evidence of the incipient role that public administration can play in promoting social enterprises' agenda that might base similar strategies to boost social enterprises in other locations.
A cold chain logistics distribution optimization model: Beijing-Tianjin-Hebei region low-carbon site selection
Authors: Zhang Liyi , Fu Mingyue , Fei Teng , Lim Ming K. , Tseng Ming-Lang 
Published: April 2024

PurposeThis study reduces carbon emission in logistics distribution to realize the low-carbon site optimization for a cold chain logistics distribution center problem.Design/methodology/approachThis study involves cooling, commodity damage and carbon emissions and establishes the site selection model of low-carbon cold chain logistics distribution center aiming at minimizing total cost, and grey wolf optimization algorithm is used to improve the artificial fish swarm algorithm to solve a cold chain logistics distribution center problem.FindingsThe optimization results and stability of the improved algorithm are significantly improved and compared with other intelligent algorithms. The result is confirmed to use the Beijing-Tianjin-Hebei region site selection. This study reduces composite cost of cold chain logistics and reduces damage to environment to provide a new idea for developing cold chain logistics.Originality/valueThis study contributes to propose an optimization model of low-carbon cold chain logistics site by considering various factors affecting cold chain products and converting carbon emissions into costs. Prior studies are lacking to take carbon emissions into account in the logistics process. The main trend of current economic development is low-carbon and the logistics distribution is an energy consumption and high carbon emissions.
Evaluation of the performance of Spanish family businesses portfolios
Authors: Otero González Luis , Querentes Hermida Raquel Esther , Durán Santomil Pablo , López Penabad Celia 
Published: April 2024

PurposeThe primary objective of this study is to analyze the performance and risk characteristics of portfolios composed of Spanish family businesses (FBs) when sustainability and quality factors are taken into account. By comparing different portfolio compositions against a benchmark, the study aims to provide insights into the impact of these factors on portfolio performance.Design/methodology/approachThis study employs an empirical approach to evaluate the performance and risk of portfolios consisting of Spanish family businesses (FBs) by incorporating sustainability and quality factors. It compares the results of various portfolios against a benchmark, utilizing GARCH models and the extended six-factor model of Fama and French for the period 2018–2023.FindingsThe findings reveal that investing in Spanish family businesses (FBs) yields higher returns compared to the index, with portfolios incorporating quality factors demonstrating superior performance. However, the inclusion of sustainability factors negatively affects portfolio performance. These results highlight the significance of considering sustainability and quality factors in portfolio construction and investment decisions.Originality/valueThis study contributes to the existing literature by examining the performance and risk implications of incorporating sustainability and quality factors into portfolios of family businesses. The findings offer valuable insights for investors and managers interested in constructing portfolios or developing financial products that balance risk and return effectively.
The e-entrepreneurial intentions of Jordanian business students to start an online business in emerging economies: an application of planned behavior theory
Authors: Abu Shriha Sofyan , AL-Shboul Moh’d Anwer , Abaddi Samer 
Published: April 2024

PurposeThe purpose of this study is to assess the correlations between the e-entrepreneurial intentions, attitude toward e-entrepreneurship, subjective norms, perceived behavior control, attitude toward risk and entrepreneurial knowledge of Jordanian business students to start an online business and the e-entrepreneurial intention.Design/methodology/approachA sample of 392 undergraduate business students from different Jordanian public and private universities participated in the study. Data were collected using an online survey-based questionnaire (i.e. Google Forms) using emails and social media platforms (i.e. WhatsApp, Facebook, etc.); reliability and validity tests were ensured. This study employs a 50-item questionnaire (distributed online via Google Forms and in two languages) to collect data, utilizing 5-point Likert scales; correlation analysis, linear regression analysis, and structural equation modeling are used to analyze the data.FindingsThe results showed that the e-entrepreneurship intentions of Jordanian business students are significantly predicted by their attitude toward e-entrepreneurship, subjective norms, perceived behavioral control, and entrepreneurial knowledge. One’s attitude toward risk does not influence the ambition to launch an Internet company much. Furthermore, their affiliation does not significantly impact the students' plans to pursue e-entrepreneurship.Practical implicationsThe study has important real-world implications, particularly for Jordan. The country could create more jobs and boost the economy by encouraging students to start online businesses and helping small businesses grow. This is especially important in Jordan, where many people, particularly young adults, struggle to find work. Therefore, true need for interventions to foster e-entrepreneurship among business students in emerging economies like Jordan.Originality/valueThe goal of this research is to examine Jordanian business students' aspirations to launch Internet businesses in developing nations throughout the digital age. The results offer valuable information on the elements influencing the e-entrepreneurial intents of Jordanian business students. This information may be utilized to create programs and policies that effectively encourage e-entrepreneurship in Jordan.
Research on small sample target detection for underwater robot
Authors: Luo Hu , Ruan Haobin , Tu Dawei 
Published: April 2024

Purpose
The purpose of this paper is to propose a whole set of methods for underwater target detection, because most underwater objects have small samples, low quality underwater images problems such as detail loss, low contrast and color distortion, and verify the feasibility of the proposed methods through experiments.

Design/methodology/approach
The improved RGHS algorithm to enhance the original underwater target image is proposed, and then the YOLOv4 deep learning network for underwater small sample targets detection is improved based on the combination of traditional data expansion method and Mosaic algorithm, expanding the feature extraction capability with SPP (Spatial Pyramid Pooling) module after each feature extraction layer to extract richer feature information.

Findings
The experimental results, using the official dataset, reveal a 3.5% increase in average detection accuracy for three types of underwater biological targets compared to the traditional YOLOv4 algorithm. In underwater robot application testing, the proposed method achieves an impressive 94.73% average detection accuracy for the three types of underwater biological targets.

Originality/value
Underwater target detection is an important task for underwater robot application. However, most underwater targets have the characteristics of small samples, and the detection of small sample targets is a comprehensive problem because it is affected by the quality of underwater images. This paper provides a whole set of methods to solve the problems, which is of great significance to the application of underwater robot.
The role of awareness of circular economy’s concept in purchase of sustainable goods and access-based and collaborative consumption – Porto case
Authors: Vieira Elvira , Pinto Borges Ana , Rodrigues Paula Lopes , Reis Ana Maria , Ostapenko Svitlana 
Published: April 2024

Purpose
Circular economy (CE) is receiving increasing worldwide attention as a manner to overcome the challenges linked to current trends of unsustainable energy and resource consumption. This paper aims to fill this gap and analyze the adherence to sustainable, access-based and collaborative consumption practices by exploring the role of CE awareness, specifically in the context of Porto, the second-largest city of Portugal.

Design/methodology/approach
The methodology of choice is quantitative, based on partial least square-based structural equation modeling.

Findings
The result shows that there is an influence of CE awareness on subsequent sustainable consumption models.

Research limitations/implications
Present research contributes to the theory on CE awareness and sustainable consumption. It proposes a model that could be applied in other countries. As this research is developed within the city of Porto, it may limit generalizations of obtained results.

Practical implications
As CE practices are embodied into national and local policies, this research contributes to understanding local contexts of CE practices dissemination, providing practical suggestions for businesses and policymakers aiming the transition to the CE.

Originality/value
An original approach to measuring the awareness of CE economy is proposed, that is analyzed not only from the familiarity perspective but in six dimensions of its construction: familiarity, importance, perception or interpretation, advantages, social impact and barriers in this process. Further, the conceptual model of the impact that these dimensions have on the adoption of sustainable consumption models (purchase of sustainable products, access-based and collaborative consumption) is proposed.
The Terminator in the goldfields: speculative affects in an extractive frontier in Colombia
Authors: Jaramillo Pablo 
Published: April 2024

AbstractThis article analyses the relationships among speculative rumour, everyday storytelling, and financial speculation that impact small‐scale gold mining towns in Colombia. By following the stories surrounding the visit of Arnold Schwarzenegger to Marmato, a mining town in central Colombia, and its consequences, I explore how people speculate about such ‘speculative’ visits so as to reframe the events as part of wider colonial histories. I aim to comprehend the affective constellation of lives criss‐crossed by financial speculation and the political openings created by rumour, storytelling, and fabulation. I argue that people locate the Schwarzenegger visit as part of a crisis‐ridden history that counters the narratives of modern and responsible mining. The argument contributes to the conceptualization of speculation in anthropology, as well as to questions of history, temporality, and politics of extractive frontiers. The article is based on ethnographic fieldwork carried out between 2016 and 2019 in the central Andes of Colombia and with governmental and corporate representatives.
Identification of prospective aging drug targets via Mendelian randomization analysis
Authors: Mao Rui , Li Ji , Xiao Wenqin 
Published: April 2024

AbstractAging represents a multifaceted process culminating in the deterioration of biological functions. Despite the introduction of numerous anti‐aging strategies, their therapeutic outcomes have often been less than optimal. Consequently, discovering new targets to mitigate aging effects is of critical importance. We applied Mendelian randomization (MR) to identify potential pharmacological targets against aging, drawing upon summary statistics from both the Decode and FinnGen cohorts, with further validation in an additional cohort. To address potential reverse causality, bidirectional MR analysis with Steiger filtering was utilized. Additionally, Bayesian co‐localization and phenotype scanning were implemented to investigate previous associations between genetic variants and traits. Summary‐data‐based Mendelian randomization (SMR) analysis was conducted to assess the impact of genetic variants on aging via their effects on protein expression. Additionally, mediation analysis was orchestrated to uncover potential intermediaries in these associations. Finally, we probed the systemic implications of drug‐target protein expression across diverse indications by MR‐PheWas analysis. Utilizing a Bonferroni‐corrected threshold, our MR examination identified 10 protein‐aging associations. Within this cohort of proteins, MST1, LCT, GMPR2, PSMB4, ECM1, EFEMP1, and ISLR2 appear to exacerbate aging risks, while MAX, B3GNT8, and USP8 may exert protective influences. None of these proteins displayed reverse causality except EFEMP1. Bayesian co‐localization inferred shared variants between aging and proteins such as B3GNT8 (rs11670143), ECM1 (rs61819393), and others listed. Mediator analysis pinpointed 1,5‐anhydroglucitol as a partial intermediary in the influence LCT exhibits on telomere length. Circulating proteins play a pivotal role in influencing the aging process, making them promising candidates for therapeutic intervention. The implications of these proteins in aging warrant further investigation in future clinical research.