bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case of Severe Lower Extremity Lymphedema Caused by Postoperative Intraperitoneal Lymphatic Leakage Treatment
Authors: Tsukura Kahori , Onoda Satoshi , Tachibana Gaku , Kobayashi Kohta , Katsuragi Ryohei , Satake Toshihiko 
Published: March 2024

Summary:
In the present study, we encountered a patient who developed intraabdominal lymphatic leakage after surgery for gastric cancer, underwent lymphangiography and lymphatic mass embolization, and developed severe lymphedema. The patient was a 55-year-old woman with gastric cancer with pancreatic invasion. Total gastrectomy and lymph node dissection were performed as conversion procedures. Six liters of ascites was detected postoperatively. Lymphangiography with Lipiodol injections into the bilateral inguinal lymph nodes was done three times, and Histoacryl embolization of the lymphatic leak was performed. However, edema of the lower extremities rapidly worsened. Lymphatic venous anastomosis was performed under general anesthesia. Anastomosis was performed at seven sites on the right and eight sites on the left. Postoperatively, the patient underwent compression therapy using the multilayer bandage method. The edema continued to improve further, and at 2 weeks postoperatively, the patient’s weight had decreased by 21.4 kg from the preoperative weight, which was the same as that before the onset of edema. In this case, the patient’s general condition was unstable due to cardiac insufficiency and other factors. Therefore, we aimed for an operation time of less than 3 hours. In addition, two surgeons performed the surgery to ensure an immediate therapeutic effect, and more anastomoses were performed than usual. After lymphatic venous anastomosis, not only the edema of both lower extremities but also the edema of the entire body improved promptly. One of the reasons for the improvement in general edema was thought to be due to increased intravascular protein and enhanced intravascular return of interstitial fluid.
Relationship Between Firm Size and Perceived Organizational Support and the Moderating Role of Supervisor Support
Authors: Inoue Shunsuke , Nagata Tomohisa , Odagami Kiminori , Adi Nuri Purwito , Mori Koji 
Published: March 2024

Objective
The study aims to explore the relationship between firm size and perceived organizational support (POS) and to investigate how supervisor support might moderate this relationship.

Methods
The study involved a cross-sectional questionnaire survey in Japan with 25,648 participants. Assessing POS through eight-question. Firm size was categorized into small (2–49 employees), medium (50–999), and large (1000 or more). Coefficients were estimated using multiple regression analyses.

Results
The analysis revealed that medium and large firms had lower POS compared to small firms, with the difference being more pronounced in medium firms. A positive interaction between firm size and supervisor support was observed for POS.

Conclusions
This study found that although POS is more likely to be lower in medium and large firms than in small firms, the effect of supervisor support on improving POS is stronger.
The role of pharmacogenomics in precision mental health care
Authors: Hamilton Joshua M. 
Published: March 2024

ABSTRACT
Pharmacogenomics is the study of how genes affect a person's response to medications. This science has given rise to the practice of “precision health care,” which combines pharmacology (the study of medication) and genomics (the study of genes and their functions) to inform a more personalized approach. Related considerations include which medications to prescribe to different individuals and what doses will have the most therapeutic effects (with the fewest side effects). A case of recalcitrant depression and anxiety (in the context of chronic inflammatory and autoimmune disease) serves as the basis for discussion of psychopharmacogenomics to inform a more effective treatment approach for psychiatric symptoms. A contemporary understanding of neurobiology and neuropathology, enhanced by genetic and genomic information, supports new models for diagnosis and precision treatment of commonly recurring mental health problems.
Impact of sex differences on the feasibility and safety of distal radial access for coronary procedures: a multicenter prospective observational study
Authors: Rivera Kristian , Fernández-Rodríguez Diego , Bullones Juan , Gorriño Susana , Sánchez Espino Alejandro , Garcia-Guimarães Marcos , Casanova-Sandoval Juan , Irigaray Patricia , Costa-Mateu Joan , Arroyo-Calpe David , Roig-Boira Oriol , Tornel-Cerezo María , Baiget-Pons Anna , Worner Fernando , Ferreiro José Luis 
Published: March 2024

BackgroundConventional transradial access in women is associated with a lower success rate and a higher incidence of spasm compared to men. To date, the effect of sex on the performance of distal radial access (DRA) has not been fully elucidated. The aim of this study was to assess the impact of sex on catheterization success and other performance parameters of DRA procedures.MethodsThis is a prospective three-center observational study. From August 2020 to September 2022, data from all consecutive patients who underwent DRA for coronary procedures were collected.ResultsA total of 868 procedures were registered and stratified into two groups according to sex: women (n = 258) and men (n = 610). Female patients had less favorable baseline characteristics than male patients in terms of absent or weak pulse (29% vs. 17%;P < 0.001), distal radial diameter (2.2 ± 0.3 vs. 2.4 ± 0.4 mm;P < 0.001) and proximal radial diameter (2.5 ± 0.7 vs. 2.7 ± 0.7 mm;P = 0.001). No differences in success rates were found in women compared to men (94.2% vs. 96.6%;P = 0.135), with a higher presence of arterial spasm in women (5.8% vs. 3.0%;P = 0.044). The preprocedural ultrasound evaluation was the only predictor of DRA success [odds ratio = 20.0 (4.739–83.333);P < 0.001].ConclusionIn patients undergoing coronary procedures, the success rate of DRA was high regardless of sex, with a higher incidence of arterial spasm in women.
Supporting the Health and Well-Being of Caregivers of Persons with Pain Strategies to address stress and improve self-care
Authors: Tonkikh Orly , Chi Nai-Ching , Herr Keela A. , Fishman Scott M. , Young Heather M. 
Published: March 2024

This article is part of a series, Supporting Family Caregivers: No Longer Home Alone, published in collaboration with the AARP Public Policy Institute. Results of focus groups, conducted as part of the AARP Public Policy Institute's No Longer Home Alone video project, supported evidence that family caregivers aren't given the information they need to manage the complex care regimens of family members. This series of articles and accompanying videos aims to help nurses provide caregivers with the tools they need to manage their family member's health care at home.
This new group of articles provides practical information nurses can share with family caregivers of persons living with pain. To use this series, nurses should read the articles first, so they understand how best to help family caregivers. Then they can refer caregivers to the informational tear sheet—Information for Family Caregivers—and instructional videos, encouraging them to ask questions. For additional information, see Resources for Nurses. Cite this article as: Tonkikh, O., et al. Supporting the Health and Well-Being of Caregivers of Persons with Pain. Am J Nurs 2023; 123 (6): 55-61.
Discrimination and Cardiovascular Health in Black Americans: Exploring Inflammation as a Mechanism and Perceived Control as a Protective Factor
Authors: Merritt Carrington C. , Muscatell Keely A. 
Published: March 2024

ABSTRACT

Objective
Inflammation may be an integral physiological mechanism through which discrimination impacts cardiovascular health and contributes to racial health disparities. Limited research has examined psychosocial factors that protect against the negative effects of discrimination on inflammation. Perceived control is a promising possible protective factor, given that it has been shown to moderate the relationship between other psychosocial stressors and physiological outcomes. This study thus tested whether systemic inflammation mediated the link between discrimination and cardiovascular health and whether perceived control moderated this relationship.

Methods
Data for this project included 347 non-Hispanic/Latinx Black adults (mean [standard deviation] age = 51.64 [11.24] years; 33% female) taken from the Midlife in the United States study. Perceived control and daily discrimination were assessed via self-report, and inflammation was measured via circulating levels of C-reactive protein (CRP), interleukin-6 (IL-6), fibrinogen, and tumor necrosis factor α. Cardiovascular health was measured by morbidity of cardiovascular conditions: heart disease, hypertension, and/or stroke.

Results
CRP (indirect effect: b = 0.004, 95% confidence interval [CI] = 0.001–0.007) and fibrinogen (indirect effect: b = 0.002, 95% CI = 0.0003–0.005) mediated the link between discrimination and cardiovascular conditions. Perceived control moderated the relationship between discrimination and CRP (F(1, 293) = 4.58, ΔR
2 = 0.013, b = −0.02, SE = 0.01, p = .033). CRP mediated the link between discrimination and cardiovascular conditions only for those who reported low levels of perceived control (Index = −0.003, 95% CI = −0.007 to −0.0001).

Conclusion
Findings provide empirical evidence of inflammation as a mechanism linking discrimination to cardiovascular conditions among Black Americans. Additionally, perceived control may be protective. Findings could suggest beliefs about control as a potential intervention target to help reduce the negative effects of discrimination on cardiovascular health among Black Americans.
Automated Breast Volume Scanner Is More Valuable Than Hand-Held Ultrasound in Diagnosis of Small Breast cancer
Authors: Yan Lixia , Jing Luxia , Lu Qing , Wang Xi , Mao Wujian , Wang Peilei , Zhan Mengna , Huang Beijian 
Published: March 2024

Abstract
This study aimed to evaluate the clinical value of automated breast volume scanner (ABVS) compared with hand-held ultrasound (HHUS). From January 2015 to May 2019, a total of 912 breast lesions in 725 consecutive patients were included in this study. κ statistics were calculated to identify interobserver agreement of ABVS and HHUS. The diagnostic performance for ABVS and HHUS was expressed as the area under the receiver operating characteristic curve, as well as the corresponding 95% confidence interval, sensitivity, and specificity. The sensitivities of ABVS and HHUS were 95.95% and 93.69%, and the specificities were 85.47% and 81.20%, respectively. A difference that nearly reached statistical significance was observed in sensitivities between ABVS and HHUS (P = 0.0525). The specificity of ABVS was significantly higher than that of HHUS (P = 0.006). When lesions were classified according to their maximum diameter, the sensitivity and specificity of ABVS were significantly higher than HHUS for lesions ≤20 mm, while they made no statistical significance between ABVS and HHUS for lesions >20 mm. The interobserver agreement for ABVS was better than that of HHUS. Automated breast volume scanner was more valuable than HHUS in diagnosing breast cancer, especially for lesions ≤20 mm, and it could be a valuable diagnostic tool for breast cancer.
Serial Nerve Conduction Studies in Guillain–Barré Syndrome: Its Usefulness and Precise Timing
Authors: Lee Hyung-Soo , Suh Bum Chun , Kim Jong Kuk , Kim Byung-Jo , Nam Tai-Seung , Oh Jeeyoung , Bae Jong Seok , Shin Kyong Jin , Kim Seung Woo , Kim Seung Min , Shin Ha Young 
Published: March 2024

Purpose:
Nerve conduction study (NCS) is essential for subclassifying Guillain–Barré syndrome (GBS). It is well known that the GBS subclassification can change through serial NCSs. However, the usefulness of serial NCSs is debatable, especially in patients with early stage GBS.

Methods:
Follow-up NCS data within 3 weeks (early followed NCS, EFN) and within 3 to 10 weeks (late-followed NCS, LFN) were collected from 60 patients with GBS who underwent their first NCS (FN) within 10 days after symptom onset. Each NCS was classified into five subtypes (normal, demyelinating, axonal, inexcitable, and equivocal), according to Hadden's and Rajabally's criteria. We analyzed the frequency of significant changes in classification (SCCs) comprising electrodiagnostic aggravation and subtype shifts between demyelinating and axonal types according to follow-up timing.

Results:
Between FN and EFN, 33.3% of patients with Hadden's criteria and 18.3% with Rajabally's criteria showed SCCs. Between FN and LFN, 23.3% of patients with Hadden's criteria and 21.7% with Rajabally's criteria showed SCCs, of which 71.4% (Hadden's criteria) and 46.2% (Rajabally's criteria) already showed SCCs from the EFN. The conditions of delayed SCCs between EFN and LFN were very early FN, mild symptoms at the FN, or persistent electrophysiological deterioration 3 weeks after symptom onset.

Conclusions:
A substantial proportion of patients with GBS showed significant changes in neurophysiological classification at the early stage. Serial NCS may be helpful for precise neurophysiological classification. This study suggests that follow-up NCSs should be performed within 3 weeks of symptom onset in patients with GBS in whom FN was performed within 10 days of symptom onset.
A dynamical perspective: moving towards mechanism in single-cell transcriptomics
Authors: Maizels Rory J. 
Published: March 2024

As the field of single-cell transcriptomics matures, research is shifting focus from phenomenological descriptions of cellular phenotypes to a mechanistic understanding of the gene regulation underneath. This perspective considers the value of capturing dynamical information at single-cell resolution for gaining mechanistic insight; reviews the available technologies for recording and inferring temporal information in single cells; and explores whether better dynamical resolution is sufficient to adequately capture the causal relationships driving complex biological systems.
This article is part of a discussion meeting issue ‘Causes and consequences of stochastic processes in development and disease’.
On epigenetic stochasticity, entropy and cancer risk
Authors: Teschendorff Andrew E. 
Published: March 2024

Epigenetic changes are known to accrue in normal cells as a result of ageing and cumulative exposure to cancer risk factors. Increasing evidence points towards age-related epigenetic changes being acquired in a quasi-stochastic manner, and that they may play a causal role in cancer development. Here, I describe the quasi-stochastic nature of DNA methylation (DNAm) changes in ageing cells as well as in normal cells at risk of neoplastic transformation, discussing the implications of this stochasticity for developing cancer risk prediction strategies, and in particular, how it may require a conceptual paradigm shift in how we select cancer risk markers. I also describe the mounting evidence that a significant proportion of DNAm changes in ageing and cancer development are related to cell proliferation, reflecting tissue-turnover and the opportunity this offers for predicting cancer risk via the development of epigenetic mitotic-like clocks. Finally, I describe how age-associated DNAm changes may be causally implicated in cancer development via an irreversible suppression of tissue-specific transcription factors that increases epigenetic and transcriptomic entropy, promoting a more plastic yet aberrant cancer stem-cell state.
This article is part of a discussion meeting issue ‘Causes and consequences of stochastic processes in development and disease’.
Cardiorespiratory demands of firearms training instruction and 15m shuttle tests in British law enforcement
Authors: Warwick Joseph , Cooper Sophie , Ronca Flaminia 
Published: March 2024

AbstractObjectivesLaw enforcement agencies require minimum fitness standards to safeguard their officers and training staff. Firearms instructors (FI) are expected to maintain the same standards as their operational counterparts. This study aimed to quantify the daily physiological demands placed on FI.Methods19 FI (45 ± 5 years) completed occupational tasks whilst wearing heart rate (HR) monitors for a minimum 10 days. Maximal oxygen consumption (VO2max) testing was conducted on FI during a treadmill test (TT) and a multistage shuttle test (ST). Linear regression models were used to model the relationship between VO2and HR throughout the TT. This model was applied to HR data from occupational tasks to infer oxygen consumption. Repeated Measures ANOVAs were used to compare time spent in VO2max equivalent zones throughout.ResultsThe VO2max achieved during ST (45.1 ± 5.6 ml/kg/min) was significantly higher than TT (39 ± 3 ml/kg/min) (p = 0.014). Time to exhaustion (TTE) was sooner on ST (06:26 min) compared to TT (13:16 min) (p < .001). FI spent ∼85% of occupational time with an oxygen demand ≤20 ml/kg/min (p < .005). The most intense occupational tasks saw FI achieve VO2max ≥30 ml/kg/min, but <40 ml/kg/min.ConclusionUsing ST to assess cardiorespiratory fitness resulted in a quicker TTE and a higher VO2max. Predominantly, FI occupational tasks are low intensity with sporadic exposures requiring a VO2max of >40 ml/kg/min. To safeguard FI from occupational-related cardiorespiratory or long-term health issues, it is intuitive to suggest fitness standards should exceed a VO2max of 40 ml/kg/min.
SEGUID v2: Extending SEGUID checksums for circular, linear, single- and double-stranded biological sequences
Authors: Pereira Humberto , Silva Paulo César , Davis M. Wayne , Abraham Louis , Babnigg György , Bengtsson Henrik , Johansson Björn 
Published: March 2024

AbstractBackgroundSynthetic biology involves combining different DNA fragments, each containing functional biological parts, to address specific problems. Fundamental gene-function research often requires cloning and propagating DNA fragments, such as those from the iGEM Parts Registry or Addgene, typically distributed as circular plasmids.Addgene’s repository alone offers around 150,000 plasmids. To ensure data integrity, cryptographic checksums can be calculated for the sequences. Each sequence has a unique checksum, making checksums useful for validation and quick lookups of associated annotations. For example, the SEGUID checksum, uniquely identifies protein sequences with a 27-character string.ObjectivesThe original SEGUID, while effective for protein sequences and single-stranded DNA (ssDNA), is not suitable for circular and double-stranded DNA (dsDNA) due to topological differences. Challenges include how to uniquely represent linear dsDNA, circular ssDNA, and circular dsDNA. To meet these needs, we propose SEGUID v2, which extends the original SEGUID to handle additional types of sequences.ConclusionsSEGUID v2 produces orientation and rotation invariant checksums for single-stranded, double-stranded, possibly staggered, linear, and circular DNA and RNA sequences. Customizable alphabets allow for other types of sequences. In contrast to the original SEGUID, which uses Base64, SEGUID v2 uses Base64url to encode the SHA-1 hash. This ensures SEGUID v2 checksums can be used as-is in filenames, regardless of platform, and in URLs, with minimal friction.AvailabilitySEGUID v2 is readily available for major programming languages, distributed under the MIT license. JavaScript packageseguidis available on npm, Python packageseguidon PyPi, R packageseguidon CRAN, and a Tcl script on GitHub. These tools, along with documentation, examples, and an onlineSEGUID Calculator, can be found athttps://www.seguid.org.
Choices to landscapes: Mechanisms of animal movement scale to landscape patterns
Authors: Rogers Will , Yanco Scott , Jetz Walter 
Published: March 2024

AbstractUnderstanding the geographic distributions of animals is central to ecological inquiry and conservation planning. Movement-based habitat selection models, like discrete-time step-selection functions, are useful for identifying key environmental attributes which animals select upon and can nearly perfectly capture preference patterns between environmental conditions. However, predictions based only on selection can often fail to accurately describe true geographic distributions. We show that by integrating local selection patterns, movement models, and explicit landscape constraints within the same framework, we can better predict simulated distributions than occurrence-based frameworks of selection alone. Using three case studies, we show that this framework can better predict distributions of organisms across increasing scales of out-of-sample prediction: within individuals, between individuals, and even between regional contexts. Movement modeling is a powerful tool to describe both the selection paradigm that organisms apply to the environment and the movement patterns that enable them to apply those selection paradigms. By understanding movement and selection at the same time, habitats that species “prefer” may not be ultimately occupied, as many “preferred” patches may be unreachable given movement strategies and landscape context, often leading to smaller geographic distributions than estimated by selection or occurrence alone.
Chlomito: a novel tool for precise elimination of organelle genome contamination in nuclear genome assemblies
Authors: Song Wei , Li Chong , Lu Yanming , Shen Dawei , Jia Yunxiao , Huo Yixin , Piao Weilan , Jin Hua 
Published: March 2024

AbstractAccurate genome assemblies are crucial for understanding biological evolution, mechanisms of disease, and biodiversity. However, contamination from organelle genomes in nuclear genome analyses often leads to inaccuracies and unreliability in results. To address this issue, we developed a tool named Chlomito, which employs innovative algorithms to precisely identify and eliminate organelle genome contamination sequences from nuclear genome assemblies. Compared to conventional approaches, Chlomito can not only detect and eliminate organelle sequences but also effectively distinguish true organelle sequences from those transferred into the nucleus via horizontal gene transfer. To evaluate the accuracy of Chlomito, we conducted tests using sequencing data from Plum and Mango. The results confirmed that Chlomito can accurately detect contigs originating from the organelle genome, and the identified contigs covered most regions of the organelle reference genomes, demonstrating its efficiency and precision in comprehensively recognizing organelle genome sequences. Additionally, for user convenience, we packaged this method into a Docker image, simplifying the data processing workflow. Overall, Chlomito provides a highly efficient and accurate method for identifying and removing contigs derived from organelle genomes in genomic assembly data, thereby contributing to the improvement of genome assembly quality and advancing research in genomics and evolutionary biology.