bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of medium sugar source on the production of retroviral vectors for gene therapy
Authors: Coroadinha A.S. , Ribeiro J. , Roldão A. , Cruz P.E. , Alves P.M. , Merten O.‐W. , Carrondo M.J.T. 
Published: March 2006

AbstractThe production of gene therapy retroviral vectors presents many difficulties, mainly due to vector instability and low cell productivities hampering the attainment of high titers of infectious viral vectors. The objective of this work is to increase the production titers of retroviral vectors by manipulating the sugar carbon sources used in bioreaction. Four sugars were tested (glucose, galactose, sorbitol, and fructose) on an established Moloney murine leukemia virus (MoMLV) producer cell line. Galactose and sorbitol did not support cell growth or vector production. Glucose supplemented at 25 mM supported the highest cell growth; however, the use of glucose or fructose at 83 and 140 mM have shown to improve the infectious vector titer three to fourfold. The reasons for the titer improvements were further analyzed and, although, the cell‐specific productivity in viral transgene RNA and reverse transcriptase were augmented 5‐ and 6‐fold for glucose at 140 mM and 14‐ and 16‐fold for fructose at 140 mM, comparing with glucose at 25 mM, these increases did not seem sufficient to account for the 14‐ (140 mM glucose) and 32‐ (140 mM fructose) fold increment obtained for the infectious particles‐specific productivity. Further accounting the enhancement in the titers was the improvement in the viral stability, the half‐life of the vectors was enhanced by 30–60%. This resulted in a product quality with a superior ratio of infectious to total particles, thus reducing the most problematic contaminant in the production of retroviral vectors, non‐infectious retroviral particles. © 2006 Wiley Periodicals, Inc.
Comparison of two immunomagnetic separation technologies to deplete T cells from human blood samples
Authors: Lara Oscar , Tong Xiaodong , Zborowski Maciej , Farag Sherif S. , Chalmers Jeffrey J. 
Published: March 2006

AbstractThe objective of this study was to compare the performance of two immunomagnetic separation technologies to deplete T cells from buffy coats of human blood. Specifically, two versions of the commercial MACS® Technology: MiniMACS and SuperMACS, and a prototype, flow‐through system, the QMS, were evaluated. Peripheral blood mononuclear leukocytes (PBL) were isolated from buffy coats and an immunomagnetic separation of CD3+ cells was conducted using company and optimized labeling protocols. To mimic peripheral blood containing bone marrow purged hematopoietic stem cells, HSC, CD34 expressing‐cells (KG1a) were spiked into PBL prior to T‐cell depletion once optimized depletion conditions were determined. Once the labeling protocol was optimized, the MiniMACS system performed well by producing a highly enriched CD3+ fraction, and a respectable level of depletion of T cells and recovery of KG1a cells in the depleted fraction; an average log10 depletion of T cells of 2.88 ± 0.17 and an average recovery of the KG1a cells of 60.8 ± 5.94% (n = 14). The performance of the SuperMACS system was very similar with an average log10 depletion of T cells of 2.89 ± 0.22 and an average recovery of KG1a of 63.1 ± 8.55% (n = 10). In contrast, the QMS system produced an average log10 depletion of T cells of 3.98 ± 0.33 (n = 16) with a corresponding average recovery of 57.9 ± 16.6% of the spiked CD34+ cells. The aforementioned QMS performance values were obtained using sorting speeds ranging from 2.5 × 104 to 1.7 × 105 cells per second. It is suggested that the lack of a 100% recovery of the unlabeled KG1a cells is the result of a previously reported “drafting” phenomena which pulls unlabeled cells in the direction of the magnetically labeled cells thereby resulting in loss of the unlabeled cells. © 2006 Wiley Periodicals, Inc.
Self‐Organizing Properties of Monosubstituted Sucrose Fatty Acid Esters: The Effects of Chain Length and Unsaturation
Authors: Molinier Valérie , Kouwer Paul J. J. , Fitremann Juliette , Bouchu Alain , Mackenzie Grahame , Queneau Yves , Goodby John W. 
Published: March 2006

AbstractThree families of mono‐substituted sucrose fatty acid esters were prepared by enzymatic and classical synthetic procedures, and their self‐assembly and self‐organizational properties were investigated by thermal polarised light microscopy, differential scanning calorimetry and X‐ray diffraction. The properties were evaluated as a function of the fatty acid chain length. For the lower homologues of the series columnar liquid‐crystalline stacking structures were found, whereas for the higher homologues, lamellar phases predominated. A model for the columnar stacking arrangement, consisting of a unique arrangement of the molecules which could lead to the creation of multiple internal ion channels between the hydrophobic interior and the hydrophilic exterior of the columns, is suggested.
A Pyrazolato‐Bridged Dinuclear Platinum(II) Complex Induces Only Minor Distortions upon DNA‐Binding
Authors: Teletchéa Stéphane , Komeda Seiji , Teuben Jan‐Maarten , Elizondo‐Riojas Miguel‐Angel , Reedijk Jan , Kozelka Jiří 
Published: March 2006

AbstractThe cytotoxic, pyrazolato‐bridged dinuclear platinum(II) complex [(cis‐{Pt(NH3)2})2(μ‐OH)(μ‐pz)]2+ (pz=pyrazolate) has been found to cross‐link two adjacent guanines of a double‐stranded DNA decamer without destabilizing the duplex and without changing the directionality of the helix axis. A 1H NMR study of the oligonucleotide d(CTCTG*G*TCTC)‐d(GAGACCAGAG), cross‐linked at the two G* guanines by [(cis‐{Pt(NH3)2})2(μ‐pz)]3+, and molecular dynamics simulations of the explicitly solvated duplex were performed to characterize the structural details of the adduct. The dinuclear platinum cross‐link unwinds the helix by approximately 15°, that is, to a similar extent as the widely used antitumor drug cisplatin, but, in contrast to the latter, induces no significant bend in the helix axis. The Watson–Crick base‐pairing remains intact, and the melting temperature of the duplex is unaffected by the cross‐link. The helical twist is considerably reduced between the two platinated bases, as becomes manifest in an unusually short sequential H1′–H1′ distance. This unwinding also affects the sugar ring of the guanosine in the 3′‐position to the cross‐link, which presents an N⇌S equilibrium. This is the first cytotoxic platinum complex that has been successfully designed by envisioning the structural consequences of its binding to DNA.
Orientational Effect of Aryl Groups on 77Se NMR Chemical Shifts: Experimental and Theoretical Investigations
Authors: Nakanishi Waro , Hayashi Satoko , Shimizu Daisuke , Hada Masahiko 
Published: March 2006

AbstractThe orientational effect of p‐YC6H4 (Ar) on δ(Se) is elucidated for ArSeR, based on experimental and theoretical investigations. The effect is examined in the cases in which SeCR in ArSeR is either in the Ar plane (pl) or is perpendicular to the plane (pd). 9‐(Arylselanyl)anthracenes (1) and 1‐(arylselanyl)anthraquionones (2) are employed to establish the effect in pl and pd, respectively. Large upfield shifts are observed for Y=NMe2, OMe, and Me, and large downfield shifts for Y=COOEt, CN, and NO2 in 1, relative to Y=H, as is expected. Large upfield shifts are brought by Y=NMe2, OMe, Me, F, Cl, and Br, and downfield shifts by Y=CN and NO2 in 2, relative to Y=H, with a negligible shift by Y=COOEt. Absolute magnetic shielding tensors of Se (σ(Se)) are calculated for ArSeR (R=H, Me, and Ph), assuming pl and pd, based on the DFT‐GIAO method. Observed characters are well explained by the total σ(Se). Paramagnetic terms (σp(Se)) are governed by (σp(Se)xx+σp(Se)yy), in which the direction of np(Se) (constructed by 4pz(Se)) is set to the z axis. The main interaction in pl is the np(Se)–π(C6H4)–pz(Y) type. The Y dependence in pl occurs through admixtures of 4pz(Se) in π(SeC6H4Y) and π*(SeC6H4Y), modified by the conjugation, with 4px(Se) and 4py(Se) in σ(CSeX) and σ*(CSeX) (X=H or C) under a magnetic field. The main interaction in pd is the σ(CSeX)–π(C6H4)–px(Y) type, in which SeX is nearly on the x axis. The Y dependence in pd mainly arises from admixtures of 4pz(Se) in np(Se) with 4px(Se) and 4py(Se) in modified σ*(CSeX), since np(Se) is filled with electrons. It is demonstrated that the effect of Y on σp(Se) in the pl conformation is the same regardless of whether Y is an electron‐donor or electron‐acceptor, whereas for pd conformations the effect is greater when Y is an electron donor, as observed in 1 and 2, respectively. Contributions of each molecular orbital and each transition on σp(Se) are evaluated, which enables us to recognize and visualize the effect clearly.
Conformational, Concomitant Polymorphs of 4,4‐Diphenyl‐2,5‐cyclohexadienone: Conformation and Lattice Energy Compensation in the Kinetic and Thermodynamic Forms
Authors: Roy Saikat , Banerjee Rahul , Nangia Ashwini , Kruger Gert J. 
Published: March 2006

Abstract4,4‐Diphenyl‐2,5‐cyclohexadienone (1) crystallized as four conformational polymorphs and a record number of 19 crystallographically independent molecules have been characterized by low‐temperature X‐ray diffraction: form A (P21, Z′=1), form B (P$\bar 1$, Z′=4), form C (P$\bar 1$, Z′=12), and form D (Pbca, Z′=2). We have now confirmed by variable‐temperature powder X‐ray diffraction that form A is the thermodynamic polymorph and B is the kinetic form of the enantiotropic system A–D. Differences in the packing of the molecules in these polymorphs result from different acidic CH donors approaching the CO acceptor in CH⋅⋅⋅O chains and in synthons I–III, depending on the molecular conformation. The strength of the CH⋅⋅⋅O interaction in a particular structure correlates with the number of symmetry‐independent conformations (Z′) in that polymorph, that is, a short CH⋅⋅⋅O interaction leads to a high Z′ value. Molecular conformation (Econf) and lattice energy (Ulatt) contributions compensate each other in crystal structures A, B, and D resulting in very similar total energies: Etotal of the stable form A=1.22 kcal mol−1, the metastable form B=1.49 kcal mol−1, and form D=1.98 kcal mol−1. Disappeared polymorph C is postulated as a high‐Z′, high‐energy precursor of kinetic form B. Thermodynamic form A matches with the third lowest energy frame based on the value of Ulatt determined in the crystal structure prediction (Cerius2, COMPASS) by full‐body minimization. Re‐ranking the calculated frames on consideration of both Econf (Spartan 04) and Ulatt energies gives a perfect match of frame #1 with stable structure A. Diphenylquinone 1 is an experimental benchmark used to validate accurate crystal structure energies of the kinetic and thermodynamic polymorphs separated by <0.3 kcal mol−1 (∼1.3 kJ mol−1).
A Dynamic Tricopper Double Helicate
Authors: Hutin Marie , Frantz Richard , Nitschke Jonathan R. 
Published: March 2006

AbstractThe reaction between 8‐aminoquinoline, 1,10‐phenantholine‐2,9‐dicarbaldehyde, and copper(I) tetrafluoroborate gave a quantitative yield of a tricopper double helicate. The presence of dynamic covalent imine (CN) bonds allowed this assembly to participate in two reactions not previously known in helicate chemistry: 1) It could be prepared through subcomponent substitution from a dicopper double helicate that contained aniline residues. An electron‐poor aniline was quantitatively displaced; a more electron‐rich aniline competed effectively with the aminoquinoline, setting up an equilibrium between dicopper and tricopper helicates that could be displaced towards the tricopper through the addition of further copper(I). 2) Both dicopper and tricopper helicates could be prepared simultaneously from a mixture of phenanthroline dialdehyde, aniline, and aminoquinoline, which contained all possible imine condensation products in equilibrium. Following the addition of copper(I), thermodynamic equilibration on both covalent and coordinative levels eliminated all partially‐formed and mixed imine ligands from the mixture, leaving the helicates as exclusive products.
On the Structure and Chemical Bonding of Tri‐Tungsten Oxide Clusters W3On‐ and W3On (n = 7—10): W3O8 as a Potential Molecular Model for O‐Deficient Defect Sites in Tungsten Oxides
Authors: Huang Xin , Zhai Hua‐Jin , Li Jun , Wang Lai‐Sheng 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Syntheses, Structure, and Bonding of Rb14(Mg1‐xInx)30. A Nonstoichiometric Phase with an Unusual Substitution in the Anion Framework.
Authors: Li Bin , Corbett John D. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Hydride Ion as a Two‐Electron Donor in a Nanoporous Crystalline Semiconductor 12CaO×7Al2O3.
Authors: Hayashi Katsuro , Sushko Peter V. , Shluger Alexander L. , Hirano Masahiro , Hosono Hideo 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Chemical Vapor Transport of Solid Solutions. Part 26. Formation of Mixed Phases and Chemical Vapor Transport in the Systems TiS2/NbS2, TiSe2/NbSe2, NbS2/TaS2 and NbSe2/TaSe2.
Authors: Hotje U. , Wartchow R. , Binnewies M. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Electrochemical and Spectroscopic Studies of the Chloro and Oxochloro Complex Formation of Nb(V) and Ta(V) in NaCl—AlCl3 Melts.
Authors: von Barner J. H. , Bjerrum N. J. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis and Characterization of Two Open‐Framework Germanates Containing Double Four‐Ring Building Units.
Authors: Lin Zhi‐En , Zheng Shou‐Tian , Yang Guo‐Yu 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
V4S9Br4: A Novel High‐Spin Vanadium Cluster Thiobromide with Square‐Planar Metal Core.
Authors: Mironov Yuri V. , Yarovoi Spartak S. , Naumov Dmitry Y. , Kozlova Svetlana G. , Ikorsky Vladimir N. , Kremer Reinhard K. , Simon Arndt , Fedorov Vladimir E. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
A New Preparative Approach to HgPbP14 Structure Type Materials: Crystal Structure of Cu0.73(1)Sn1.27(1)P14 and Characterization of M1‐xSn1+xP14 (M: Cu, Ag) and AgSbP14.
Authors: Lange Stefan , Sebastian C. Peter , Nilges Tom 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis, Structure, and Magnetic Properties of Sr2NiOsO6 and Ca2NiOsO6: Two New Osmium‐Containing Double Perovskites.
Authors: Macquart Rene , Kim Seung‐Joo , Gemmill William R. , Stalick Judith K. , Lee Yongjae , Vogt Tom , zur Loye Hans‐Conrad 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Al‐Catalyzed Enantioselective Alkylation of α‐Ketoesters by Dialkylzinc Reagents. Enhancement of Enantioselectivity and Reactivity by an Achiral Lewis Base Additive.
Authors: Wieland Laura C. , Deng Hongbo , Snapper Marc L. , Hoveyda Amir H. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Asymmetric Multicomponent Domino Reactions and Highly Enantioselective Conjugated Addition of Thiols to α,β‐Unsaturated Aldehydes.
Authors: Marigo Mauro , Schulte Tobias , Franzen Johan , Joergensen Karl Anker 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Asymmetric Organocatalysis with Novel Chiral Thiourea Derivatives: Bifunctional Catalysts for the Strecker and Nitro‐Michael Reactions.
Authors: Tsogoeva Svetlana B. , Yalalov Denis A. , Hateley Martin J. , Weckbecker Christoph , Huthmacher Klaus 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Asymmetric Direct Vinylogous Michael Reaction of Activated Alkenes to Nitroolefins Catalyzed by Modified Cinchona Alkaloids.
Authors: Xue Dong , Chen Ying‐Chun , Wang Qi‐Wei , Cun Ling‐Feng , Zhu Jin , Deng Jin‐Gen 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Diastereoselective Synthesis of Five‐ and Seven‐Membered Rings by [2 + 2 + 1], [3 + 2], [3 + 2 + 2], and [4 + 3] Carbocyclization Reactions of β‐Substituted (Alkenyl)(methoxy)carbene Complexes with Methyl Ketone Lithium Enolates.
Authors: Barluenga Jose , Alonso Jorge , Fananas Francisco J. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
One‐Pot Synthesis of New Imidazo[1,2‐a]pyridine and Imidazo[1,2‐a]pyrimidine Derivatives.
Authors: Georgescu Florentina , Georgescu Emilian , Draghici Constantin , Iuhas Paula C. , Filip Petru I. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Novel Nitrogen‐Containing Chelating Ligands from Aziridines — Preparation, Coordination Studies, and Catalytic Application in the Cyclopropanation of Styrene.
Authors: Krasnova Larissa B. , Yudin Andrei K. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Kumada Coupling of Aryl and Vinyl Tosylates under Mild Conditions.
Authors: Limmert Michael E. , Roy Amy H. , Hartwig John F. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Quinolinium Fluorochromate: An Efficient and Convenient Reagent for Oxidative Cleavage of Thioacetals to Their Parent Carbonyl Compounds.
Authors: Tajbakhsh Mahmood , Alimohammadi Kamal , Ramzanian‐Lehmali Farhad , Barghamadi Mohamad , Shakeri Alireza 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Four‐Component Pd‐Catalyzed Cascade/Ring Closing Metathesis. Synthesis of Heterocyclic Enones.
Authors: Grigg Ronald , Martin William , Morris James , Sridharan Visuvanathar 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Sulfur Dioxide Mediated One‐Pot, Four‐Component Synthesis of Polyfunctional Sulfones and Sulfonamides, Including Medium‐Ring Cyclic Derivatives.
Authors: Bouchez Laure C. , Turks Maris , Dubbaka Srinivas Reddy , Fonquerne Freddy , Craita Cotinica , Laclef Sylvain , Vogel Pierre 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
β Elimination of a Phosphonate Group from an Alkoxyl Radical — Intramolecular Acylation Using Acylphosphonate Derivatives as Carbonyl Group Acceptors.
Authors: Cho Chang Ho , Kim Sunggak 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Regioselective Nitration of N,N‐Dialkylanilines Using Cerium(IV) Ammonium Nitrate in Acetonitrile.
Authors: Yang Xianghua , Xi Chanjuan , Jiang Yanfeng 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
A Powerful Broensted Acid Catalyst for the Organocatalytic Asymmetric Transfer Hydrogenation of Imines.
Authors: Hoffmann Sebastian , Seayad Abdul Majeed , List Benjamin 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis, Structure Determination, and Hydroformylation Activity of N‐Heterocyclic Carbene Complexes of Rhodium.
Authors: Chen Austin C. , Allen Daryl P. , Crudden Cathleen M. , Wang Ruiyao , Decken Andreas 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Asymmetric Phase Transfer Catalyzed Glycolate Alkylation: Investigation of the Scope, and Application to the Synthesis of (‐)‐Ragaglitazar.
Authors: Andrus Merritt B. , Hicken Erik J. , Stephens Jeffrey C. , Bedke D. Karl 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Ruthenium‐Catalyzed Asymmetric Hydrogenation of α‐ and β‐Keto Esters in Ionic Liquids Using Chiral P‐Phos Ligand.
Authors: Lam Kim Hung , Xu Lijin , Feng Lichun , Ruan Jiwu , Fan Qinghua , Chan Albert S. C. 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Mild and Reversible Dehydration of Primary Amides with PdCl2 in Aqueous Acetonitrile.
Authors: Maffioli Sonia I. , Marzorati Ettore , Marazzi Alessandra 
Published: March 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.