bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of onabotulinum toxin‐A injection on sexual function in women with refractory interstitial cystitis/bladder pain syndrome: A prospective study
Authors: Karaburun Murat Can , Kubilay Eralp , Öztuna Derya , Gökçe Mehmet İlker , Süer Evren , Gülpınar Ömer 
Published: March 2024

AbstractObjectivesTo determine the effect of intravesical onabotulinum toxin‐A (BoNT‐A) treatment on sexual functions in female patients with refractory interstitial cystitis/bladder pain syndrome (IC/BPS).MethodsFemale patients with IC/BPS refractory to previous treatments were included in the study between January 2020 and April 2022. Patients were treated with the trigone‐sparing injection (Group 1) or trigone‐included injection (Group 2) techniques. 100 Units of BoNT‐A was applied submucosally on 20 different points. The patients were evaluated with visual analog scale (VAS), O'Leary‐Sant Interstitial Cystitis Symptom Index (ICSI), Interstitial Cystitis Problem Index (ICPI), Female Sexual Function Index (FSFI) questionnaires, 3‐day voiding diary, uroflowmetry, and post‐voiding residual volume analysis in the preoperative period, as well as on the 30th and 90th days postoperatively. For the repeated measurements, analysis of variance was used to assess the time‐dependent variation across groups.ResultsThe baseline FSFI score of the patients was 15.96 ± 3.82. Following the treatment, the FSFI scores were 22.43 ± 4.93 and 24.41 ± 5.94 on the 30th and 90th days, respectively (p < .001). We observed statistically significant improvement in all FSFI subdomains (p < .05). Statistically significant improvements with treatment on ICSI, ICPI, and VAS scores were achieved (p < .05). Preoperative FSFI scores were similar in Group 1 and Group 2 (p = .147). While the preoperative FSFI scores were 17.00 ± 3.73 and 14.84 ± 3.72 for Group 1 and Group 2, respectively, the scores after the treatment were 22.85 ± 5.01 and 21.98 ± 5.01 on the 30th day, and 24.62 ± 6.06 and 24.19 ± 6.05 on the 90th day postoperatively. Significant improvement was observed in FSFI scores with treatment, and no difference was observed between the two groups in terms of treatment response (p = .706).ConclusionsIntravesical BoNT‐A injection in the treatment of women with refractory IC/BPS improves sexual functions. It also significantly improves pain and symptom scores. Both trigone‐sparing and trigone‐including injections are similarly safe and effective.
Machine learning‐based prediction of tear osmolarity for contact lens practice
Authors: Garaszczuk Izabela K. , Romanos‐Ibanez Maria , Consejo Alejandra 
Published: March 2024

AbstractPurposeThis study addressed the utilisation of machine learning techniques to estimate tear osmolarity, a clinically significant yet challenging parameter to measure accurately. Elevated tear osmolarity has been observed in contact lens wearers and is associated with contact lens‐induced dry eye, a common cause of discomfort leading to discontinuation of lens wear.MethodsThe study explored machine learning, regression and classification techniques to predict tear osmolarity using routine clinical parameters. The data set consisted of 175 participants, primarily healthy subjects eligible for soft contact lens wear. Various clinical assessments were performed, including symptom assessment with the Ocular Surface Disease Index and 5‐Item Dry Eye Questionnaire (DEQ‐5), tear meniscus height (TMH), tear osmolarity, non‐invasive keratometric tear film break‐up time (NIKBUT), ocular redness, corneal and conjunctival fluorescein staining and Meibomian glands loss.ResultsThe results revealed that simple linear regression was insufficient for accurate osmolarity prediction. Instead, more advanced regression models achieved a moderate level of predictive power, explaining approximately 32% of the osmolarity variability. Notably, key predictors for osmolarity included NIKBUT, TMH, ocular redness, Meibomian gland coverage and the DEQ‐5 questionnaire. In classification tasks, distinguishing between low (<299 mOsmol/L), medium (300–307 mOsmol/L) and high osmolarity (>308 mOsmol/L) levels yielded an accuracy of approximately 80%. Key parameters for classification were similar to those in regression models, emphasising the importance of NIKBUT, TMH, ocular redness, Meibomian glands coverage and the DEQ‐5 questionnaire.ConclusionsThis study highlights the potential benefits of integrating machine learning into contact lens research and practice. It suggests the clinical utility of assessing Meibomian glands and NIKBUT in contact lens fitting and follow‐up visits. Machine learning models can optimise contact lens prescriptions and aid in early detection of conditions like dry eye, ultimately enhancing ocular health and the contact lens wearing experience.
Diet‐dependent reproductive investment in gumleaf skeletonizer moths, Uraba lugens
Authors: Phạm Hiếu ThỊ , McNamara Kathryn B. , Elgar Mark A. 
Published: March 2024

AbstractJuvenile diet can profoundly affect subsequent adult development, morphology and reproductive investment. Yet, little is known about how juvenile diet affects adult investment into chemical‐based sexual signalling, perhaps due to the historical assumption that pheromone production is not costly. We explored how juvenile diet influenced the reproductive investment of adults in the gumleaf skeletonizer moth, Uraba lugens. Juveniles were reared on different host plant species (Eucalyptus camaldulensis and Eucalyptus moorei) and on E. moorei host plants with different fertilizer treatments (fertilized and non‐fertilized). These juvenile diets differ in foliage carbon and nitrogen content. Several adult life history traits were influenced by juvenile diet, including body size in females, and longevity in males. However, we found no evidence from Y‐maze olfactometer assays that diet affected the attractiveness of female pheromones to males. Finally, host plant species affected male pre‐copulatory investment: males reared on E. moorei had longer antennae, but less dense sensilla. Combined, our experiments suggest that the effects of juvenile diet on investment in reproductive traits, including those associated with signalling, differ between males and females. Females allocate nutrients to adult body size, which determines fecundity. In contrast, males allocate nutrients to adult longevity and antennae size, both of which improve mate search and mating success.
Ideology shapes evaluation of history within the general population
Authors: Rigoli Francesco 
Published: March 2024

AbstractThe way history is interpreted varies across ideologies as articulated by political thinkers and by leading politicians. Is history also assessed differently by laypeople reporting different ideological orientations? To address this, the article describes a study from six countries where, in addition to reporting their ideology on a left–right spectrum, participants evaluated the recent past, the present, and the near future. The data show that, in all countries, right‐ compared to left‐wing supporters evaluated the past as more positive. To elucidate this effect, a second study manipulated the appraisal of the past between groups but found that this did not influence participants' ideology. A third study manipulated the salience of ideological representations between groups. Here, the high‐salience group displayed a stronger link between ideology and evaluation of the past, indicating that embracing a certain ideology encourages a specific interpretation of the past. Exploring the factors mediating this effect, one last study found that nostalgia for tradition partially explains why right‐wing supporters cherish the past more. Altogether, these observations show that how history is interpreted is central not only to intellectuals' writings and politicians' speeches but also to laypeople's political beliefs.
A Beautiful Death
Authors: Blankenship‐Lai Paul Houston 
Published: March 2024

ABSTRACTThis article is about the death of a graduate school for spiritual formation and theological education. I ask what the death of this school can teach us about teaching theology and religion for the sake of student transformation and more loving pedagogies. I also ask how, when an institution dies, it can die beautifully. The underlying thesis of this article is that there is, alive in our classrooms (and ourselves, perhaps), a spiritual wound that can be named an experiential death of divine love—and that this wound is manifesting as unshepherded fear, rage, and discontent. I suggest that this spiritual wound be tended through skillful pedagogical tenderness, flexibility, and liberative co‐creation with students to cultivate a presence of love and feed the spiritual hungers of our time. I also suggest that a school (and a teacher) dies beautifully when its death is allowed and free to become a scene of beautiful instruction.
Ratiometric gibberellin biosensors for the analysis of signaling dynamics and metabolism in plant protoplasts
Authors: Andres Jennifer , Schmunk Lisa J. , Grau‐Enguix Federico , Braguy Justine , Samodelov Sophia L. , Blomeier Tim , Ochoa‐Fernandez Rocio , Weber Wilfried , Al‐Babili Salim , Alabadí David , Blázquez Miguel A. , Zurbriggen Matias D. 
Published: March 2024

SUMMARYGibberellins (GAs) are major regulators of developmental and growth processes in plants. Using the degradation‐based signaling mechanism of GAs, we have built transcriptional regulator (DELLA)‐based, genetically encoded ratiometric biosensors as proxies for hormone quantification at high temporal resolution and sensitivity that allow dynamic, rapid and simple analysis in a plant cell system, i.e. Arabidopsis protoplasts. These ratiometric biosensors incorporate a DELLA protein as a degradation target fused to a firefly luciferase connected via a 2A peptide to a renilla luciferase as a co‐expressed normalization element. We have implemented these biosensors for all five Arabidopsis DELLA proteins, GA‐INSENSITIVE, GAI; REPRESSOR‐of‐ga1‐3, RGA; RGA‐like1, RGL1; RGL2 and RGL3, by applying a modular design. The sensors are highly sensitive (in the low pm range), specific and dynamic. As a proof of concept, we have tested the applicability in three domains: the study of substrate specificity and activity of putative GA‐oxidases, the characterization of GA transporters, and the use as a discrimination platform coupled to a GA agonists' chemical screening. This work demonstrates the development of a genetically encoded quantitative biosensor complementary to existing tools that allow the visualization of GA in planta.
Dominance complementation of parental heading date alleles of Hd1, Ghd7, DTH8, and PRR37 confers transgressive late maturation in hybrid rice
Authors: Zong Wubei , Song Yingang , Xiao Dongdong , Guo Xiaotong , Li Fuquan , Sun Kangli , Tang Wenjing , Xie Wenhao , Luo Yanqiu , Liang Shan , Zhou Jingyao , Xie Xianrong , Liu Dilin , Chen Letian , Wang Haiyang , Liu Yao‐Guang , Guo Jingxin 
Published: March 2024

SUMMARYRice (Oryza sativa L.) is a short‐day plant whose heading date is largely determined by photoperiod sensitivity (PS). Many parental lines used in hybrid rice breeding have weak PS, but their F1 progenies have strong PS and exhibit an undesirable transgressive late‐maturing phenotype. However, the genetic basis for this phenomenon is unclear. Therefore, effective methods are needed for selecting parents to create F1 hybrid varieties with the desired PS. In this study, we used bulked segregant analysis with F1 Ningyou 1179 (strong PS) and its F2 population, and through analyzing both parental haplotypes and PS data for 918 hybrid rice varieties, to identify the genetic basis of transgressive late maturation which is dependent on dominance complementation effects of Hd1, Ghd7, DTH8, and PRR37 from both parents rather than from a single parental genotype. We designed a molecular marker‐assisted selection system to identify the genotypes of Hd1, Ghd7, DTH8, and PRR37 in parental lines to predict PS in F1 plants prior to crossing. Furthermore, we used CRISPR/Cas9 technique to knock out Hd1 in Ning A (sterile line) and Ning B (maintainer line) and obtained an hd1‐NY material with weak PS while retaining the elite agronomic traits of NY. Our findings clarified the genetic basis of transgressive late maturation in hybrid rice and developed effective methods for parental selection and gene editing to facilitate the breeding of hybrid varieties with the desired PS for improving their adaptability.
Preventive nasal administration of flagellin restores antimicrobial effect of gentamicin and protects against a multidrug-resistant strain of
Pseudomonas aeruginosa
Authors: Maia Ana Raquel , Cezard Adeline , Fouquenet Delphine , Vasseur Virginie , Briard Benoit , Sirard Jean-Claude , Si-Tahar Mustapha , Hervé Virginie 
Published: March 2024

ABSTRACT

The increasing prevalence of multidrug-resistant
Pseudomonas aeruginosa
(PA) is a significant concern for chronic respiratory disease exacerbations. Host-directed drugs, such as flagellin, an agonist of toll-like receptor 5 (TLR5), have emerged as a promising solution. In this study, we evaluated the prophylactic intranasal administration of flagellin against a multidrug-resistant strain of PA (PA
MDR
) in mice and assessed the possible synergy with the antibiotic gentamicin (GNT). The results indicated that flagellin treatment before infection decreased bacterial load in the lungs, likely due to an increase in neutrophil recruitment, and reduced signs of inflammation, including proinflammatory cytokines. The combination of flagellin and GNT showed a synergistic effect, decreasing even more the bacterial load and increasing mice survival rates, in comparison to mice pre-treated only with flagellin. These findings suggest that preventive nasal administration of flagellin could restore the effect of GNT against MDR strains of PA, paving the way for the use of flagellin in vulnerable patients with chronic respiratory diseases.
Structural and functional changes of binocular corneal innervation and ocular surface function after unilateral SMILE and tPRK
Authors: Gong Qianwen , Huang Kaiyan , Li Kexin , Tong Yixuan , Zhao Jian , Wang Hui , Xu Zhiqiang , Lin Meng , Lu Fan , Hu Liang 
Published: March 2024

Aims
To evaluate the bilateral changes in the sub-basal nerve plexus of the cornea and ocular surface function after unilateral small incision lenticule extraction (SMILE) and transepithelial photorefractive keratectomy (tPRK) procedures.

Methods
34 patients were enrolled in the study and underwent unilateral SMILE (21 of 34 patients) or unilateral tPRK (13 of 34 patients). Complete ophthalmic examinations, tear film function tests and Cochet-Bonnet esthesiometry were conducted to assess the effects of the surgeries on the corneal nerves and tear function. Morphological changes were assessed using in vivo confocal microscopy to evaluate the corneal sub-basal nerve plexus and dendritic cells. ELISA was used to measure the tear neuromediators. Clinical and morphological data at each follow-up point were compared with preoperative baseline values.

Results
All patients who underwent unilateral SMILE or tPRK procedures exhibited bilateral corneal nerve degenerative changes, decreased corneal sensitivity, worsening of dry eye symptoms and changes in bilateral tear neuromediators. In the SMILE group, bilateral corneal sensitivity was positively correlated with corneal nerve fibre length and negatively correlated with dendritic cell area. The dry eye severity was negatively correlated with corneal sensitivity. Tear levels of substance P and nerve growth factor were positively correlated with mean dendritic cell area and dry eye severity, but negatively correlated with corneal sensitivity. In the tPRK group, bilateral corneal sensitivity was positively correlated with corneal nerve fibre density.

Conclusions
Unilateral refractive surgery may bilaterally affect the morphology and function of corneal nerves and ocular surface status postoperatively.
Understanding the role of storage reserve mobilization during seed germination and initial seedling growth in species of the genus Carapa
Authors: de Carvalho Josiane C. , Nascimento Gleisson de O. , Fernandes Andreia V. , Gonçalves Elmer V. , dos Santos Pedro Paulo , Santos Alberdan S. , Gonçalves José Francisco de C. 
Published: March 2024

A physiological and metabolic database for various species of Amazonian plants is crucial to the species selection and sustainable utilization of their diversity. We hypothesized that understanding the mobilization of Carapa seed reserves and water uptake during germination could provide insights into the physiological behavior and propagation to differentiate species of the same genus. Given this, the mobilization mechanisms of primary reserves, water uptake, and enzymatic activities in seeds of two species of Carapa ( Carapa guianensis and Carapa vasquezii) were investigated. The lipid content was high in both species, with a value of 63.7 ± 4.7% for C. guianensis and 55 ± 1.5% for C. vasquezii. Carapa guianensis showed higher values for the other evaluated reserves. The protein profile highlights a band between 15 and 25 kDa in C. guianensis. As for the enzymatic activities investigated (lipase, amylase, protease, amd acid phosphatase), C. guianensis showed greater activity for most of them, except for acid phosphatase. Overall, the species showed different metabolic strategies and dynamics regarding the water uptake and use of reserves. Our findings indicate that distinct metabolic pathways are employed during the germination and initial seedling growth stages of two Carapa species. This trait can potentially guide the use and conservation of these species.
Targeted Gene Deletion or Antagonism of the Prostaglandin E2 EP3 Receptor Protects Against Cardiac Injury Postmyocardial Infarction
Authors: Bryson Timothy D. , Bhat Shaheen Y. , Moore Carlin , Taube David , Xu Jiang , Peterson Edward , Harding Pamela 
Published: March 2024

BACKGROUND:
Prostaglandin E2 acts through 4 G-protein-coupled receptors (EP1–EP4). We previously reported that activation of the EP3 receptor reduces cardiac contractility, and its expression increases after a myocardial infarction (MI), mediating the reduction in cardiac function. In contrast, cardiac overexpression of the EP4 receptor in MI substantially improves cardiac function. Moreover, we recently reported that mice overexpressing EP3 have heart failure under basal conditions and worsened cardiac function after MI. Thus, the deleterious effects of the prostaglandin E2 EP receptors in the heart are mediated via its EP3 receptor. We, therefore, hypothesized that cardiomyocyte-specific knockout (CM-EP3 KO) or antagonism of the EP3 receptor protects the heart after MI.

METHODS:
To test our hypothesis, we made the novel CM-EP3 KO mouse and subjected CM-EP3 KO or controls to sham or MI surgery for 2 weeks. In separate experiments, C57BL/6 mice were subjected to 2 weeks of MI and treated with either the EP3 antagonist L798 106 or vehicle starting 3 days post-MI.

RESULTS:
CM-EP3 KO significantly prevented a decline in cardiac function after MI compared with WT animals and prevented an increase in hypertrophy and fibrosis. Excitingly, mice treated with L798 106 3 days after MI had significantly better cardiac function compared with vehicle-treated mice.

CONCLUSIONS:
Altogether, these data suggest that EP3 may play a direct role in regulating cardiac function, and pharmaceutical targeting of the EP3 receptor may be a therapeutic option in the treatment of heart failure.
Moving and Static Faces, Bodies, Objects, and Scenes Are Differentially Represented across the Three Visual Pathways
Authors: Küçük Emel , Foxwell Matthew , Kaiser Daniel , Pitcher David 
Published: March 2024

Abstract
Models of human cortex propose the existence of neuroanatomical pathways specialized for different behavioral functions. These pathways include a ventral pathway for object recognition, a dorsal pathway for performing visually guided physical actions, and a recently proposed third pathway for social perception. In the current study, we tested the hypothesis that different categories of moving stimuli are differentially processed across the dorsal and third pathways according to their behavioral implications. Human participants (n = 30) were scanned with fMRI while viewing moving and static stimuli from four categories (faces, bodies, scenes, and objects). A whole-brain group analysis showed that moving bodies and moving objects increased neural responses in the bilateral posterior parietal cortex, parts of the dorsal pathway. By contrast, moving faces and moving bodies increased neural responses, the superior temporal sulcus, part of the third pathway. This pattern of results was also supported by a separate ROI analysis showing that moving stimuli produced more robust neural responses for all visual object categories, particularly in lateral and dorsal brain areas. Our results suggest that dynamic naturalistic stimuli from different categories are routed in specific visual pathways that process dissociable behavioral functions.
Roles of the Default Mode Network in Different Aspects of Self-representation When Remembering Social Autobiographical Memories
Authors: Katsumi Azusa , Iwata Saeko , Tsukiura Takashi 
Published: March 2024

Abstract
Autobiographical memory (AM) is episodic memory for personally experienced events, in which self-representation is more important than that in laboratory-based memory. Theoretically, self-representation in a social context is categorized as the interpersonal self (IS) referred to in a social interaction with a person or the social-valued self (SS) based on the reputation of the self in the surrounding society. Although functional neuroimaging studies have demonstrated the involvement of the default mode network (DMN) in self-representation, little is known about how the DMN subsystems contribute differentially to IS-related and SS-related AMs. To elucidate this issue, we used fMRI to scan healthy young adults during the recollection of AMs. We performed multivariate pattern analysis (MVPA) and assessed functional connectivity in the DMN subsystems: the midline core, medial temporal lobe (MTL), and dorsomedial pFC (dmPFC) subsystems. The study yielded two main sets of findings. First, MVPA revealed that all DMN subsystems showed significant classification accuracy between IS-related and nonsocial-self-related AMs, and IS-related functional connectivity of the midline core regions with the retrosplenial cortex of the MTL subsystem and the dmPFC of the dmPFC subsystem was significant. Second, MVPA significantly distinguished between SS-related and nonsocial-self-related AMs in the midline core and dmPFC subsystems but not in the MTL subsystem, and SS-related functional connectivity with the midline core regions was significant in the temporal pole and TPJ of the dmPFC subsystem. Thus, dissociable neural mechanisms in the DMN could contribute to different aspects of self-representation in social AMs.