bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of drug‐eluting stents with bare metal stents in unselected patients with acute myocardial infarction
Authors: Kupferwasser L. Iri , Amorn Allen M. , Kapoor Nikhil , Lee Michael S. , Kar Saibal , Cercek Bojan , Dohad Suhail , Mirocha James , Forrester James S. , Shah Prediman K. , Makkar Raj R. 
Published: June 2007

AbstractObjectives:The aim of this study was to compare the procedural characteristics and outcomes of patients with acute myocardial infarction treated with drug‐eluting stents (DES) vs. bare metal stents (BMS).Background:DES have been shown to reduce the incidence of restenosis and target vessel revascularization (TVR) in clinical randomized studies when compared with BMS in patients undergoing elective percutaneous intervention. Limited data are available with the use of DES in patients with acute ST‐segment elevation myocardial infarction.Methods:Two hundred and sixty‐one consecutive patients who presented with myocardial infarction between 7/2001 and 8/2005 were studied. The procedural characteristics, 30‐day and 12‐month outcomes of 131 patients treated with DES were compared with 130 patients treated with BMS.Results:At 12‐months follow‐up DES therapy was associated with a substantial decrease in major adverse cardiovascular events (MACE) (HR 0.33; P =0.002), TVR (HR 0.19; P =0.002), and recurrent myocardial infarction (HR 0.23; P =0.051) vs. BMS therapy. Coronary interventions utilizing DES were characterized by a marked increase in the number of stent per target vessel (DES: 1.9 ± 0.9 vs. BMS: 1.38 ± 0.6, P < 0.0001), treatment of bifurcation (DES: 21% vs. BMS: 5%, P =0.0004), and multivessel intervention (DES: 22% vs. BMS: 8%, P =0.003).Conclusion:The routine use of DES in acute myocardial infarction is associated with reduced rates of MACE at 12 months vs BMS, despite a higher rate of complex procedures in the DES treated patients. In addition to its anti‐restenosis effect, the improved outcome of patients treated with DES may be linked to a more complete revascularization in association with prolonged clopidogrel therapy. © 2007 Wiley‐Liss, Inc.
Tryptophan‐to‐Dye Fluorescence Energy Transfer Applied to Oxygen Sensing by Using Type‐3 Copper Proteins
Authors: Zauner Gerhild , Lonardi Emanuela , Bubacco Luigi , Aartsma Thijs J. , Canters Gerard W. , Tepper Armand W. J. W. 
Published: June 2007

AbstractA fluorescence‐based system to sense oxygen in solution is described. The method exploits the sensitivity of the endogenous fluorescence of type‐3 copper proteins towards the presence of oxygen by translating the near‐UV emission of the protein to label fluorescence in the visible range through a FRET mechanism. The main protein in this study, a recombinant tyrosinase from the soil bacterium Streptomyces antibioticus, has been covalently labeled with a variety of fluorescent dye molecules with emission maxima spanning the whole visible wavelength range. In all cases, the emission of the label varied considerably between O2‐bound and O2‐free protein with a contrast exceeding that of the Trp emission for some labels. It is shown that different constructs may be simultaneously observed using a single excitation wavelength. Next to the described application in oxygen sensing, the method may be applicable to any protein showing variations in tryptophan fluorescence, for example as a function of ligand binding or catalysis.
Tetraazaperopyrenes: A New Class of Multifunctional Chromophores
Authors: Riehm Till , De Paoli Gabriele , Konradsson Asgeir E. , De Cola Luisa , Wadepohl Hubert , Gade Lutz H. 
Published: June 2007

AbstractTetraazaperopyrene and a range of derivatives have been synthesised and their photophysical and redox‐chemical properties studied. The parent compound, 1,3,8,10‐tetraazaperopyrene (1), was prepared by treating 4,9‐diamino‐3,10‐perylenequinone diimine with triethyl orthoformate, whereas the 2,9‐disubstituted derivatives of 1 were obtained after treatment with the corresponding carboxylic acid chloride or anhydride (2 mol equiv). The 1,3,8,10‐tetraazaperopyrene core structure was established by X‐ray diffraction of 2,9‐bis(2‐bromophenyl)‐1,3,8,10‐tetraazaperopyrene (6). The UV‐visible absorption spectra of the compounds have a characteristic visible π*←π absorption band at 440 nm (log εmax=4.80) with a strong vibrational progression (Δν≈1450 cm−1). Diprotonation of the nitrogen atoms induces a bathochromic shift of this band from 430–440 to 470–480 nm and all four nitrogen atoms are protonated when pure H2SO4 is used as the solvent. The first and second as well as the third and fourth protonations occur concomitantly, which implies that they have very similar pKa values and, consequently, similar proton affinities. A theoretical study of the proton affinities in the gas phase and in solution attributes this behaviour to the effects of polar solvents, which dampen the charge of a protonated site at the other end of the molecule and thus effectively decouple the two opposite pyrimidine units in the polycondensed aromatic compound. The photophysical data were modelled in a time‐dependent DFT study of 1, 1H22+ and 1H44+ in both the gas phase and in a polar solvent. All the dyes show weak fluorescence in organic solvents, however, their protonated conjugate acids show dramatically increased fluorescence intensity. All of the dyes undergo two electrochemically reversible one‐electron reductions with cyclovoltammetric half‐wave potentials at Ered1≈−0.9 V and Ered2≈−1.3 V (vs. SCE), which are associated with characteristic spectral changes.
Heavy Grignard Reagents: Challenges and Possibilities of Aryl Alkaline Earth Metal Compounds
Authors: Westerhausen Matthias , Gärtner Martin , Fischer Reinald , Langer Jens , Yu Lian , Reiher Markus 
Published: June 2007

AbstractCompounds of the type arylMX, with M=Ca, Sr, Ba and X as any kind of ligand (such as halide, phosphanide, amide, aryl), are presented. The low reactivity of the heavy alkaline earth metals calcium, strontium, and barium enforces an activation prior to use for the direct synthesis. The insertion of these metals into CI bonds of aryl iodides (direct synthesis) yields aryl metal iodides and has to be performed at low temperatures and in THF. Aryl alkaline‐earth‐metal compounds show some characteristics: 1) the ease of ether cleavage enforces low reaction temperatures, 2) for Sr and Ba the Schlenk equilibrium is shifted towards homoleptic MI2 and MPh2, 3) high solubility of diaryl alkaline‐earth‐metal derivatives in THF even at low temperatures initiated quantum chemical investigations on the aggregation behavior, and 4) a strong low field shift of the 13C resonances of the ipso carbon atoms in NMR spectra was observed. First results from quantum chemical calculations on diaryl dicalcium(I) suggest a long CaCa bond with a considerable CaCa bond dissociation energy. Initial results on a selection of applications such as metallation, metathesis, and addition reactions of aryl calcium compounds are presented as well.
Rapid screening of enzymes for the enzymatic hydrolysis of chiral esters in drug discovery
Authors: Petucci Chris , Di Li , McConnell Oliver 
Published: June 2007

AbstractThirty‐five enzymes were rapidly screened for their ability to selectively hydrolyze chiral esters to their corresponding carboxylic acids for the efficient generation of chiral intermediates in drug discovery. Optimization of the enzymatic reactions at various incubation times was performed using a robotic liquid handler. Enantiomeric pairs of chiral esters and carboxylic acids were then analyzed simultaneously by chiral GC/MS in a single analysis. This analytical approach is particularly useful for compounds that do not possess a conjugated chromophore or are volatile and difficult to analyze by chiral HPLC/UV or HPLC/MS. The resulting data was used to determine enantiomeric excesses and percent conversions to the desired enantiomer of the carboxylic acid for the selection of efficient enzymes for bioconversions in drug discovery in a pharmaceutical company. Chirality, 2007. © 2007 Wiley‐Liss, Inc.
Linked claims and medical records for cancer case management
Authors: Eberl Margaret M. , Watroba Nancy , Reinhardt Marc , Pomerantz Jay , Serghany Joseph , Broffman Gregory , Fox Chester H. , Mahoney Martin C. , Edge Stephen B. 
Published: June 2007

AbstractBACKGROUND.Failure to obtain the requisite follow‐up of patients with abnormal mammograms may delay cancer diagnosis and impact outcome. Up to 20% of women do not receive timely recommended follow‐up. The current study tested the accuracy of the linkage of payer claims and clinical data to identify the appropriate treatment for patients with abnormal mammograms.METHODS.Electronic medical records in a staff model practice that was affiliated with a single health payer were scanned to identify the Breast Imaging Reporting and Data System (BI‐RADS®) code for all mammograms. For each woman with a BI‐RADS code 0, 3, 4, or 5 mammogram, the payer claims were searched for follow‐up breast procedures (imaging, biopsy, and surgery) occurring within 2 months for BI‐RADS code 0, 4, and 5 mammograms and within 8 months for BI‐RADS code 3 mammograms. For women with >1 abnormal mammogram during the study period, only follow‐up for the first abnormal mammogram was examined. The medical records of cases defined by claims as not having recommended follow‐up care were reviewed to determine the accuracy of claims analyses.RESULTS.A total of 17,329 women underwent 23,721 mammograms between January 1, 2001 and December 31, 2003. BI‐RADS codes 0, 3, 4, or 5 occurred in 1490 mammograms (6.3%). Among 1206 women with a first abnormal mammogram who were eligible for claims follow‐up, 16% did not receive recommended follow‐up care. Medical record review demonstrated that the claims search accurately identified follow‐up care in 97% of these cases.CONCLUSIONS.Administrative claims supplemented with BI‐RADS data were found to accurately identify the follow‐up care of patients with abnormal mammograms. Case management using this method may assist physicians in ensuring that all patients receive appropriate care. Cancer 2007. © 2007 American Cancer Society.
Gemcitabine‐based combinations for inoperable pancreatic cancer: Have we made real progress?
Authors: Bria Emilio , Milella Michele , Gelibter Alain , Cuppone Federica , Pino Maria Simona , Ruggeri Enzo Maria , Carlini Paolo , Nisticò Cecilia , Terzoli Edmondo , Cognetti Francesco , Giannarelli Diana 
Published: June 2007

AbstractBACKGROUND.Several attempts have been made at improving the efficacy of gemcitabine in advanced pancreatic cancer by combining it with other chemotherapeutic or molecularly targeted agents. However, randomized trials have produced conflicting results.METHODS.All prospective, randomized, phase 3 trials that compared single‐agent gemcitabine with gemcitabine‐based combinations were considered eligible for the current analysis. A literature‐based meta‐analysis was performed, event‐based relative risk ratios with 95% confidence intervals were derived through both a fixed‐effect model approach and a random‐effect model approach, and overall survival (OS) was explored as the primary endpoint. To estimate the magnitude of the eventual benefit, absolute differences and the number of patients needed to treat (NNT) for 1 patient to benefit were calculated. A sensitivity analysis for OS was performed according to the type of agent used in combination with gemcitabine.RESULTS.Twenty trials that involved 6296 patients were identified. No significant differences in the primary endpoint were observed in the overall population or in the sensitivity analysis. Conversely, a significant advantage was evident with regard to both progression‐free survival (PFS) and the overall response rate (ORR) in the overall population, with an absolute benefit of 2.6% (NTT = 39 patients) and 3.0% (NNT = 33 patients). Platinum combinations led to the greatest absolute benefits for PFS and ORR compared with single‐agent gemcitabine (10% and 6.5%, respectively), but this did not result in an OS benefit. Improvement in PFS, but not in the ORR, was correlated with an improvement in OS.CONCLUSIONS.Single‐agent gemcitabine remains the standard of care for patients with advanced pancreatic cancer. However, platinum/gemcitabine combinations appeared to improve PFS and the ORR and, thus, may be considered in selected patients. Cancer 2007. © 2007 American Cancer Society.
Long‐term outcomes after treatment with brachytherapy and supplemental conformal radiation for prostate cancer patients having intermediate and high‐risk features
Authors: Dattoli Michael , Wallner Kent , True Lawrence , Cash Jennifer , Sorace Richard 
Published: June 2007

AbstractBACKGROUND.This study summarizes long‐term outcomes from treatment of prostate cancer with increased risk of extracapsular cancer extension (ECE) using brachytherapy‐based treatment.METHODS.A total of 282 consecutive patients were treated from 1992–1996 by 1 author (M.D.). Two hundred forty‐three patients had at least 1 higher risk feature for ECE including Gleason Score 7–10 (172), prostate‐specific antigen (PSA) above 10 (166), and clinical stages T2c (109) and T3 (107). Using National Comprehensive Cancer Network (NCCN) guidelines, 119 patients had intermediate‐risk disease and 124 had high‐risk disease. Patients received pelvic 3‐dimensional conformal external beam radiation followed by a palladium (Pd)‐103 boost. Generous brachytherapy margins were utilized. Biochemical failure was defined using ASTRO Consensus Definition, nadir +2 and PSA >0.2 ng/mL at last follow‐up. The nonfailing patient follow‐up period was 1–14 years (median, 9.5 years). Biochemical data and original biopsy slides were independently re‐reviewed at the University of Washington (by K.W. and L.T., respectively).RESULTS.Overall actuarial freedom from biochemical progression at 14 years was 81%, including 87% and 72% having intermediate and high‐risk disease, respectively. Absolute risk of failure decreased progressively, falling to 1% beyond 6 years after treatment. All failing patients had prostate biopsies without evidence of local recurrence. The strongest predictor of failure was Gleason score (P = .03) followed by PSA (P = .041). Treatment morbidity was limited to temporary RTOG grade 1–2 urinary and gastrointestinal symptoms.CONCLUSIONS.High tumor control rates are possible with beam radiation followed by Pd‐103 brachytherapy. Despite perceptions that brachytherapy is inappropriate for patients at higher risk for ECE, this series strengthens the rationale that brachytherapy‐based treatment may be a desirable modality for such patients. Cancer 2007. © 2007 American Cancer Society.
The Nature and Efficiency of Neurotransmitter Exocytosis also Depend on Physicochemical Parameters
Authors: Amatore Christian , Arbault Stéphane , Guille Manon , Lemaître Frédéric 
Published: June 2007

AbstractExocytosis is an important biological process used by cells to deliver messengers or effectors to target cells with high spatial, quantitative, and kinetic precision. This process occurs by interaction and fusion of vesicles containing the (bio)chemical information with the cell membrane to release their contents into the surrounding medium. Because of its importance for life, this mechanism underlies many biological controlling factors, including different families of proteins and enzymes. Tremendous efforts have been made over the last decade toward their determination. However, in parallel, many studies have also shown that the physical and chemical characteristics of the exocytosis actors (vesicle, membrane, and extracellular medium) could directly affect the quantitative or kinetic features of secretion. The major pieces evidence for this influence, which have been reported in the literature, are reviewed herein. It demonstrates undoubtedly that pure biological aspects cannot be segregated from the physicochemical context of living mechanisms.
Liquid‐based endometrial cytology in the management of sonographically thickened endometrium
Authors: Buccoliero Anna M. , Gheri Chiara F. , Castiglione Francesca , Garbini Francesca , Fambrini Massimiliano , Bargelli Gianni , Barbetti Alfredo , Pappalardo Salvatore , Taddei Antonio , Boddi Vieri , Scarselli Gian F. , Marchionni Mauro , Taddei Gian L. 
Published: June 2007

AbstractLiquid‐based cytology represents an opportunity to re‐evaluate endometrial cytology. We evaluated the accuracy of liquid‐based endometrial cytology as compared to biopsy in 670 women scheduled for histeroscopy because of thickened endometrium (>4 mm), as evaluated by transvaginal sonography. Endometrial biopsy detected pathology in 41 (6%) of cases (21 of which were adenocarcinomas). Cytologic study found pathology in 62 (9%) cases (19 of which were adenocarcinomas). Two hundred ninety‐one biopsies (43%) and 28 (4%) cytologies were inadequate. The sensitivity and the specificity were estimated, respectively, at 95% and 98%; the positive and negative predictive values were estimated, respectively, at 83% and 99%. Cytology provided sufficient material more often than biopsy (P < 0.01). We consider endometrial cytology an efficacious diagnostic opportunity. It could be usefully applied in association with transvaginal sonography. The combination of these procedures might reduce more invasive and expensive diagnostic procedures. Diagn. Cytopathol. 2007;35:398–402. © 2007 Wiley‐Liss, Inc.
Pseudoangiosarcomatous features in medullary thyroid carcinoma spindle‐cell variant. Report of a case studied by FNA and immunohistochemistry
Authors: Laforga Juan B. , Aranda F. Ignacio 
Published: June 2007

AbstractWe report a case of medullary thyroid carcinoma (MTC), spindle cell variant, which exhibited striking histological pseudovascular clefts and abortive lumina, closely mimicking an angiosarcoma. The patient is a 72‐yr‐old‐man who presented facial rash and a solid nodule in the right lobe of the thyroid gland. The fine‐needle aspiration (FNA) showed bloody background containing loosely groups of fusiform and plasmocytoid cells with coarse chromatin and eosinophilic granular cytoplasms. Microscopically, the tumor exhibited spindle‐cell pattern intermingled with a striking angiosarcoma‐like pattern characterized by the presence of abortive lumen and clefts containing erythrocytes. Dense hyaline extracellular amyloid was present. The tumor cells were strongly positive for cytokeratin, chromogranine‐A, synaptophysine, serotonin, calcitonin, and CEA. TTF‐1 was weakly positive. Stains for CD34 and CD31 were negative. This case illustrates that the spindle cell variant of MTC may exhibit an infrequent angiosarcoma‐like appearance which may be misdiagnosed as angiosarcoma. Diagn. Cytopathol. 2007;35:424‐428. © 2007 Wiley‐Liss, Inc.
ThinPrep Pap tests in patients with endometrial cancer: A histo‐cytological correlation
Authors: Zhou Jianhong , Tomashefski Joseph F. , Khiyami Amer 
Published: June 2007

AbstractThe aim of this retrospective study was to correlate cytological diagnoses of endometrial cancers in ThinPrep Pap tests with the histological diagnoses. ThinPrep specimens from 67 patients within 12 mo of the histological diagnosis of endometrial cancer were studied. Of this study sample, 89.6% had abnormal Pap tests. Abnormal Pap tests occurred in 96.8, 68.4, and 100% of patients with grades 1, 2, or 3 endometrial cancers, respectively. Of patients with endocervical involvement, 88.9% had positive or suspicious Pap tests, compared with 41.1% without endocervical involvement (LR = 7.85, P < 0.01). Of patients with ≥50% myometrial invasion, 78.9% had positive or suspicious Pap tests, compared with 34.8% with less than 50% invasion (LR = 10.97, P < 0.01). Positive or suspicious Pap tests were found in 59.5 and 32.1% of those with tumors ≥3 cm or <3cm, respectively (LR = 4.85, P < 0.05). Diagn. Cytopathol. 2007;35:448–453. © 2007 Wiley‐Liss, Inc.
Estimated cardiac vagal tone predicts fetal responses to mother's and stranger's voices
Authors: Smith Laura S. , Dmochowski Pawel A. , Muir Darwin W. , Kisilevsky Barbara S. 
Published: June 2007

AbstractHeart rate responses of 84 near‐term fetuses to recorded female voices were examined in 166 trials of auditory stimulation. Each fetus was presented with a 2‐min recording of their mother's voice and a 2‐min recording of a female stranger's voice, in counterbalanced order, with a 10‐min rest period between trials. High frequency heart rate variability during a 2‐min baseline period was used to estimate cardiac vagal tone for each trial. Differential heart rate responses to familiar and unfamiliar voice recordings were observed during a 2‐min poststimulus period, only when estimated cardiac vagal tone was high. This finding suggests that vagal tone plays a moderating role in the cardiac responses of term fetuses to familiar and unfamiliar stimuli. © 2007 Wiley Periodicals, Inc. Dev Psychobiol 49: 543‐547, 2007.
Measuring salivary cortisol in studies of child development: Watch out—what goes in may not come out of saliva collection devices
Authors: Harmon Amanda G. , Hibel Leah C. , Rumyantseva Olga , Granger Douglas A. 
Published: June 2007

AbstractTechnical advances that enable the noninvasive measurement of biomarkers in saliva have spawned a generation of investigations that integrate biological variables into behavioral and developmental research. This study examines whether the collection of saliva, using common absorbent devices compromises the measurement of cortisol when saliva specimens have low sample volume. Within subjects (n = 20), saliva samples were prepared to experimentally represent a gradient of lower to higher sample volumes. One aliquot was immediately frozen (no treatment control) and the remaining aliquots were absorbed (“collected”) using one of three collection techniques employed in studies of child development (e.g., braided cotton dental rope, Salivette cotton pledget, or hydrocellulose microsponge). The sample volume recovered from each device relative to the initial volume available to be absorbed, and cortisol level recovered from each device relative to the untreated‐control condition were measured. Results reveal that for certain collection devices (1) the percent volume recovered is related to the initial volume available to be absorbed, (2) a substantial percentage of cortisol in saliva specimens can remain in absorbent material, and (3) the percent of cortisol recovered can be associated with the initial sample volume available to be absorbed. When research participants, such as young children, produce low volume saliva specimens, some absorbent devices may have the potential to introduce error variance in the measurement of salivary cortisol. © 2007 Wiley Periodicals, Inc. Dev Psychobiol 49: 495‐500, 2007.