bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neamine Inhibits Prostate Cancer Growth by Suppressing Angiogenin-Mediated rRNA Transcription
Authors: Ibaragi Soichiro , Yoshioka Norie , Li Shuping , Hu Miaofen G. , Hirukawa Saori , Sadow Peter M. , Hu Guo-fu 
Published: March 2009

Abstract
Purpose: Angiogenin (ANG) undergoes nuclear translocation and stimulates rRNA transcription in both prostate cancer cells and endothelial cells. The purpose of this study is to assess the antitumor activity of neamine, a nontoxic degradation product of neomycin that blocks nuclear translocation of ANG.
Experimental Design: The anti-prostate cancer activity of neamine was first evaluated in a xenograft animal model. It was then examined in the murine prostate-restricted AKT transgenic mice that develop prostate intraepithelial neoplasia (PIN) owing to AKT transgene overexpression.
Results: Neamine inhibits xenograft growth of PC-3 human prostate cancer cells in athymic mice. It blocks nuclear translocation of ANG and inhibits rRNA transcription, cell proliferation, and angiogenesis. Neamine also prevents AKT-induced PIN formation as well as reverses fully developed PIN in murine prostate-restricted AKT mice, accompanied by a decrease in rRNA synthesis, cell proliferation, and angiogenesis and an increase in prostate epithelial cell apoptosis.
Conclusion: We confirmed that ANG is a molecular target for cancer drug development and that blocking nuclear translocation of ANG is an effective means to inhibit its activity. Our results also suggested that neamine is a lead compound for further preclinical evaluation.
A Negative Feedback Regulatory Loop Associates the Tyrosine Kinase Receptor ERBB2 and the Transcription Factor GATA4 in Breast Cancer Cells
Authors: Hua Guoqiang , Zhu Bing , Rosa Fréderic , Deblon Nicolas , Adélaïde José , Kahn-Perlès Brigitte , Birnbaum Daniel , Imbert Jean 
Published: March 2009

Abstract
Overexpression of the ERBB2 gene, linked to genomic and transcriptional amplifications, is a poor prognosis indicator in 25% to 30% of breast cancers. In contrast to some well-documented genomic amplifications, molecular mechanisms leading to ERBB2 transcriptional overexpression remain poorly characterized. Gene expression analyses of breast cancer have characterized distinct transcriptional signatures allowing a molecular classification of breast carcinoma. Coexpression of the ERBB2 and GATA4 genes was originally observed in tumors. Both genes are essential for cardiovascular development and GATA4 has been proposed to control the transcription of critical genes for the differentiation and the function of myocardium. We determined that ERBB2-targeted small interfering RNA repressed both ERBB2 and GATA4 genes, whereas GATA4-targeted small interfering RNA repressed GATA4 and activated ERBB2 transcription. Transfected GATA4-expressing construct repressed ERBB2 promoter. Phylogenetic foot printing revealed multiple putative GATA4 binding sites conserved in mammals within the ERBB2 promoter region. Chromatin immunoprecipitation showed that GATA4 binds specifically to several ERBB2 gene noncoding regions. Electrophoretic mobility shift assay revealed GATA4 binding to a well-conserved consensus motif. Site-directed mutagenesis confirmed the role of this new regulatory element for the activity of the ERBB2 gene enhancer. In agreement with a repressor role of GATA4 on ERBB2 gene expression balanced by ERBB2 activation of the GATA4 gene, a negative correlation between the relative levels of ERBB2 and GATA4 mRNA was observed in breast cancer cell lines and breast tumor samples. We propose that the negative feedback loop linking ERBB2 and GATA4 plays a role in the transcriptional dysregulation of ERBB2 gene expression in breast cancer. (Mol Cancer Res 2009;7(3):402–14)
Galectin-1-Mediated Tumor Invasion and Metastasis, Up-Regulated Matrix Metalloproteinase Expression, and Reorganized Actin Cytoskeletons
Authors: Wu Ming-Heng , Hong Tse-Ming , Cheng Hui-Wen , Pan Szu-Hua , Liang Yu-Ray , Hong Hsiao-Chin , Chiang Wei-Fan , Wong Tung-Yiu , Shieh Dar-Bin , Shiau Ai-Li , Jin Ying-Tai , Chen Yuh-Ling 
Published: March 2009

Abstract
Galectin-1 (Gal-1) is a β-galactose-binding lectin; its expression level has been reported to correlate with tumor progression. Gal-1 is highly expressed in the invasive front of primary tumors and in the cancer cells of metastatic lesions in the lymph nodes of patients with oral squamous cell carcinoma. However, the molecular mechanism of Gal-1 in tumor metastasis is not completely clear. We found that increased Gal-1 expression is closely associated with its high levels of invasion in lung adenocarcinoma and oral squamous cell carcinoma cell lines. Knocking down Gal-1 with small interfering RNA in highly invasive cancer cells reduced their invasion levels. Moreover, the invasion ability of poorly invasive cancer cells was significantly increased after Gal-1 overexpression of Gal-1. Mechanism studies revealed that Gal-1 promoted tumor invasion mainly by up-regulating matrix metalloproteinase (MMP)-9 and MMP-2 and by reorganizing actin cytoskeleton. Gal-1 enhanced the activation of Cdc42, a small GTPase and member of the Rho family, thus increasing the number and length of filopodia on tumor cells. Furthermore, Gal-1-overexpressing cells had higher metastatic abilities in tail vein metastasis assays in vivo. We conclude that Gal-1 is involved in tumor invasion and metastasis by increasing MMP expression and reorganizing cytoskeletons in oral cancers and lung adenocarcinoma. (Mol Cancer Res 2009;7(3):311–8)
Equatorial Zonal Jet Formation through the Barotropic Instability of Low-Frequency Mixed Rossby–Gravity Waves, Equilibration by Inertial Instability, and Transition to Superrotation
Authors: Fruman Mark D. , Hua Bach Lien , Schopp Richard 
Published: March 2009

Abstract
Depth-dependent barotropic instability of short mixed Rossby–gravity (MRG) waves is proposed as a mechanism for the formation of equatorial zonal jets. High-resolution primitive equation simulations show that a single MRG wave of very short zonal wavelength and small to moderate amplitude is unstable and leads to the development of a largely barotropic, zonally symmetric flow, featuring a westward jet at the equator and extra-equatorial jets alternating in direction with latitude. At higher but still moderate amplitude, westward flow still prevails at the equator at depths of maximum horizontal velocity amplitude in the initial wave, but the long-term equilibrated state can also feature eastward “superrotating” jets at the equator near the depths of zero horizontal velocity in the initial wave. The formation of the superrotating jets in the simulations is found to be sensitive to the inclusion of the nontraditional Coriolis force in the equations of motion. A linear theory is used to demonstrate the existence of exponentially growing horizontally nondivergent perturbations with a dominant zonally symmetric zonal velocity component. An argument for the sense and positioning of the jets relative to the equator is given in terms of inertial instability and the meridional mixing of planetary vorticity by the small zonal-scale components of the linearly unstable modes. In the long time evolution of the flow, if the amplitude of the westward equatorial jet becomes too great, zonally symmetric inertial instability limits the growth of the jets, and inertial adjustment leads to the homogenization of potential vorticity in latitude and depth around the equator.
A study to determine whether the use of a preconnect urinary catheter system reduces the incidence of nosocomial urinary tract infections
Authors: Madeo M. , Barr B. , Owen E. 
Published: March 2009

Nosocomial urinary tract infection (NUTI) is a common problem in the UK and is often related to the use of urinary catheters. In the UK urinary catheters account for approximately 25% of all healthcare associated infections and can have a considerable financial burden on the health service. Bacteria may gain entry into the bladder of a catheterised patient via the intra-luminal route especially if accidental disconnection arises. A prospective study was undertaken to determine the NUTI rates in patients using a preconnected catheter system compared to a traditional bag and catheter system on three medical wards. A total of 205 patients were included in the study. The catheter-associated urinary tract infection rate (CAUTI) in the baseline was 37.8 per 1,000 catheter days and 22.4 per 1,000 catheter days in the intervention group. The rates of CAUTI were 41% lower in the intervention group. The data in this sample group suggests the use of the preconnected catheter offers some protection against infection. The use of the preconnected system is a new concept in the UK and further studies are required to evaluate its effectiveness. The results from this study suggest their use may help to reduce the risk of CAUTI in certain clinical areas.
Long-term tracheostomy care: How to do it
Authors: Björling Gunilla 
Published: March 2009

Educational aimsTo provide an overview of long-term tracheostomy care.To increase the understanding of long-term tracheostomy care.To increase knowledge about tube selection.To improve understanding of how to avoid serious complicationsSummaryThe overall aim for long-term tracheostomy care is to help those with respiratory failure to achieve a high-quality active life. A long-term tracheostomy does not necessarily mean an increased need for hospital care. With an optimally fitted tracheostomy tube, patient and staff education and regular follow-ups, serious complications can be avoided. This article describes predictors of good long-term tracheostomy care, such as tube selection, indications of change and follow-up.
A role for CD81 on the late steps of HIV-1 replication in a chronically infected T cell line
Authors: Grigorov Boyan , Attuil-Audenis Valérie , Perugi Fabien , Nedelec Martine , Watson Sarah , Pique Claudine , Darlix Jean-Luc , Conjeaud Hélène , Muriaux Delphine 
Published: March 2009

AbstractBackgroundHIV-1 uses cellular co-factors for virion formation and release. The virus is able to incorporate into the viral particles host cellular proteins, such as tetraspanins which could serve to facilitate HIV-1 egress. Here, we investigated the implication of several tetraspanins on HIV-1 formation and release in chronically infected T-lymphoblastic cells, a model that permits the study of the late steps of HIV-1 replication.ResultsOur data revealed that HIV-1 Gag and Env structural proteins co-localized with tetraspanins in the form of clusters. Co-immunoprecipitation experiments showed that Gag proteins interact, directly or indirectly, with CD81, and less with CD82, in tetraspanin-enriched microdomains composed of CD81/CD82/CD63. In addition, when HIV-1 producing cells were treated with anti-CD81 antibodies, or upon CD81 silencing by RNA interference, HIV-1 release was significantly impaired, and its infectivity was modulated. Finally, CD81 downregulation resulted in Gag redistribution at the cell surface.ConclusionOur findings not only extend the notion that HIV-1 assembly can occur on tetraspanin-enriched microdomains in T cells, but also highlight a critical role for the tetraspanin CD81 on the late steps of HIV replication.
Toxicogenomic analysis of susceptibility to inhaled urban particulate matter in mice with chronic lung inflammation
Authors: Thomson Errol M , Williams Andrew , Yauk Carole L , Vincent Renaud 
Published: March 2009

AbstractBackgroundIndividuals with chronic lung disease are at increased risk of adverse health effects from airborne particulate matter. Characterization of underlying pollutant-phenotype interactions may require comprehensive strategies. Here, a toxicogenomic approach was used to investigate how inflammation modifies the pulmonary response to urban particulate matter.ResultsTransgenic mice with constitutive pulmonary overexpression of tumour necrosis factor (TNF)-α under the control of the surfactant protein C promoter and wildtype littermates (C57BL/6 background) were exposed by inhalation for 4 h to particulate matter (0 or 42 mg/m3EHC-6802) and euthanized 0 or 24 h post-exposure. The low alveolar dose of particles (16 μg) did not provoke an inflammatory response in the lungs of wildtype mice, nor exacerbate the chronic inflammation in TNF animals. Real-time PCR confirmed particle-dependent increases of CYP1A1 (30–100%), endothelin-1 (20–40%), and metallothionein-II (20–40%) mRNA in wildtype and TNF mice (p < 0.05), validating delivery of a biologically-effective dose. Despite detection of striking genotype-related differences, including activation of immune and inflammatory pathways consistent with the TNF-induced pathology, and time-related effects attributable to stress from nose-only exposure, microarray analysis failed to identify effects of the inhaled particles. Remarkably, the presence of chronic inflammation did not measurably amplify the transcriptional response to particulate matter.ConclusionOur data support the hypothesis that health effects of acute exposure to urban particles are dominated by activation of specific physiological response cascades rather than widespread changes in gene expression.
Application of the Cloze Procedure to Evaluate Comprehension and Demonstrate Rewriting of Pharmacy Educational Materials
Authors: Miller Michael J , DeWitt Jane E , McCleeary Erin M , O'keefe Kelly J 
Published: March 2009

Background: Written materials are commonly used to communicate pharmacy-relevant information to patients. However, they are often composed at a level that limits comprehension, mitigating a well-intended effect. Objective: To (1) use the cloze procedure (a test designed to assess reading comprehension) to evaluate an individual's understanding of a pharmacy-relevant educational pamphlet; (2) compare results of the cloze procedure with the reading comprehension component of the Short Test of Functional Health Literacy in Adults (S-TOFHLA); and (3) use results to demonstrate rewriting of the educational pamphlet. Methods: The cloze procedure was applied to a pharmacy-relevant educational pamphlet describing safe medication practices. A total of 162 subjects were recruited from university faculty, staff, and students; a local adult literacy center; and community senior centers. Subjects completed a background interview, the S-TOFHLA. and cloze procedure for the pharmacy-relevant educational pamphlet. S-TOFHLA and cloze procedure scores were described and compared. Cloze procedure responses were used to demonstrate revision of the pamphlet RESULTS: Of the 154 subjects analyzed, mean ± SD age was 56.5 ± 20.4 years. Subjects were predominantly white (93.5%), female (71.4%). and college graduates (42.2%). Mean score on the S-TOFHLA was 92.1%. A majority (95.5%, 147/154) of subjects demonstrated adequate functional health literacy. In contrast, mean score on the cloze procedure was 53.3%. Internal consistencies of the S-TOFHLA and the cloze procedure were 0.92 and 0.90, respectively. Scores on the cloze procedure and the S-TOFHLA were highly correlated (r = 0.71, p < 0.001), Performance on the cloze procedure indicated that 55.2% of subjects required supplemental teaching. Conclusions: In this highly educated, health-literate sample, a majority did not understand the pharmacy-relevant educational pamphlet despite adequate performance on a standard measure of health literacy. The cloze procedure can be used to assess comprehension of educational materials, solicit feedback from intended users, and guide the revision of educational materials.
An evaluation of the bioavailability and aquatic toxicity attributed to ambient zinc concentrations in fresh surface waters from several parts of the world
Authors: van Genderen Eric , Adams William , Cardwell Rick , Volosin Joe , Santore Robert , Rodriguez Patricio 
Published: March 2009

Abstract
Ambient concentrations of metals in surface waters have become an important consideration when establishing water quality criteria and conducting risk assessments. This study sought to estimate amounts of zinc that may be released into freshwater considering ambient concentrations, toxicity thresholds, and bioavailability. Cumulative distribution functions of ambient zinc concentrations were compared statistically for streams and lakes in Europe, North America, and South America to identify differences among mean distribution variables (e.g., slopes, intercepts, and inflection points). Results illustrated that most of the distributions among sites differed significantly. These differences illustrate the variability in ambient zinc concentrations in surface waters because of geographic location, regional geology, and anthropogenic influence. Additionally, water quality data were used to estimate bioavailable zinc concentrations in ambient surface waters (based on predictions using biotic ligand models). The amount of dissolved metal that could be added to surface waters without exceeding toxicity thresholds was calculated by subtracting ambient surface water concentrations from chronic no observable effect concentrations (NOEC; reproduction for Daphnia magna) or 10% effective concentrations (EC10; growth rate for Pseudokirchneriella subcapitata). Because ambient dissolved-zinc concentrations were, on average, below predicted effects thresholds, an average of 57.1 ± 175 μg/L (±SD) of zinc could be added before exceeding the D. magna chronic NOEC or the P. subcapitata chronic EC10. However, numerous sites (17%) were identified as having ambient zinc concentrations in excess of these toxicity thresholds. This article uses existing biotic ligand models for zinc to estimate the potential magnitudes and variabilities of bioavailable zinc concentrations in fresh surface waters from different regions of the world.
Gene Deletion of the Kinin Receptor B1 Attenuates Cardiac Inflammation and Fibrosis During the Development of Experimental Diabetic Cardiomyopathy
Authors: Westermann Dirk , Walther Thomas , Savvatis Konstantinos , Escher Felcicitas , Sobirey Meike , Riad Alexander , Bader Michael , Schultheiss Heinz-Peter , Tschöpe Carsten 
Published: March 2009

OBJECTIVE
Diabetic cardiomyopathy is associated with increased mortality in patients with diabetes. The underlying pathology of this disease is still under discussion. We studied the role of the kinin B1 receptor on the development of experimental diabetic cardiomyopathy.

RESEARCH DESIGN AND METHODS
We utilized B1 receptor knockout mice and investigated cardiac inflammation, fibrosis, and oxidative stress after induction of streptozotocin (STZ)-induced diabetes. Furthermore, the left ventricular function was measured by pressure-volume loops after 8 weeks of diabetes.

RESULTS
B1 receptor knockout mice showed an attenuation of diabetic cardiomyopathy with improved systolic and diastolic function in comparison with diabetic control mice. This was associated with a decreased activation state of the mitogen-activated protein kinase p38, less oxidative stress, as well as normalized cardiac inflammation, shown by fewer invading cells and no increase in matrix metalloproteinase-9 as well as the chemokine CXCL-5. Furthermore, the profibrotic connective tissue growth factor was normalized, leading to a reduction in cardiac fibrosis despite severe hyperglycemia in mice lacking the B1 receptor.

CONCLUSIONS
These findings suggest that the B1 receptor is detrimental in diabetic cardiomyopathy in that it mediates inflammatory and fibrotic processes. These insights might have useful implications on future studies utilizing B1 receptor antagonists for treatment of human diabetic cardiomyopathy.
Recurrent Antecedent Hypoglycemia Alters Neuronal Oxidative Metabolism In Vivo
Authors: Jiang Lihong , Herzog Raimund I. , Mason Graeme F. , de Graaf Robin A. , Rothman Douglas L. , Sherwin Robert S. , Behar Kevin L. 
Published: March 2009

OBJECTIVE
The objective of this study was to characterize the changes in brain metabolism caused by antecedent recurrent hypoglycemia under euglycemic and hypoglycemic conditions in a rat model and to test the hypothesis that recurrent hypoglycemia changes the brain's capacity to utilize different energy substrates.

RESEARCH DESIGN AND METHODS
Rats exposed to recurrent insulin-induced hypoglycemia for 3 days (3dRH rats) and untreated controls were subject to the following protocols: [2-13C]acetate infusion under euglycemic conditions (n = 8), [1-13C]glucose and unlabeled acetate coinfusion under euglycemic conditions (n = 8), and [2-13C]acetate infusion during a hyperinsulinemic-hypoglycemic clamp (n = 8). In vivo nuclear magnetic resonance spectroscopy was used to monitor the rise of13C-labeling in brain metabolites for the calculation of brain metabolic fluxes using a neuron-astrocyte model.

RESULTS
At euglycemia, antecedent recurrent hypoglycemia increased whole-brain glucose metabolism by 43 ± 4% (P &lt; 0.01 vs. controls), largely due to higher glucose utilization in neurons. Although acetate metabolism remained the same, control and 3dRH animals showed a distinctly different response to acute hypoglycemia: controls decreased pyruvate dehydrogenase (PDH) flux in astrocytes by 64 ± 20% (P = 0.01), whereas it increased by 37 ± 3% in neurons (P = 0.01). The 3dRH animals decreased PDH flux in both compartments (−75 ± 20% in astrocytes, P &lt; 0.001, and −36 ± 4% in neurons, P = 0.005). Thus, acute hypoglycemia reduced total brain tricarboxylic acid cycle activity in 3dRH animals (−37 ± 4%, P = 0.001), but not in controls.

CONCLUSIONS
Our findings suggest that after antecedent hypoglycemia, glucose utilization is increased at euglycemia and decreased after acute hypoglycemia, which was not the case in controls. These findings may help to identify better methods of preserving brain function and reducing injury during acute hypoglycemia.
Dysrhythmia Management Content in ADN and BSN Curricula
Authors: McIntosh Trisha , Duske Shawna , Anderson Mary Ann , Hill Pamela 
Published: March 2009

Background:
The purposes of this study were to compare nurse educators' perceptions of the importance of selected dysrhythmia management competencies for the graduates of associate degree in nursing (ADN) and baccalaureate of science in nursing (BSN) programs, and to compare what related content is taught at the ADN and BSN level for dysrhythmia management.

Method:
A quantitative, nonexperimental design with a mailed survey was used to compare how nurse educators perceive the importance of dysrhythmia competencies in selected ADN and BSN programs in the states of Illinois and Iowa, and to determine what related content is taught.

Results:
A total of 33 ADN and 24 BSN programs returned the survey for a 58.2% (57 of 98) response rate. Results indicated that ADN and BSN faculty in Illinois and Iowa perceived that selected dysrhythmia management content is important and that such content is currently provided at both the ADN and BSN level.

Conclusions:
Health care institutions can have confidence that new graduates from either educational preparation have had dysrhythmia management content and can expand their level of expertise.

J Contin Educ Nurs
2008;39(7):328–333.