bluuebunny/crossref_metadata_2025_split huggingface dataset:
A roadmap for virtual professional learning: Bringing inquiry science practices to life through teacher professional community
Authors: Haavind Sarah 
Published: February 2024

AbstractAccording to the Next Generation Science Standards, three‐dimensional teaching challenges educators to adopt an inquiry approach through science and engineering practices aligned with disciplinary core ideas and crosscutting concepts. Few teachers today feel confident in their ability to orchestrate such lessons. Teaching inquiry science takes time and hard work. It also requires collaborative professional learning communities that support teachers in overcoming typical classroom challenges. This article describes an effective approach for facilitating professional learning that supports inquiry science teaching. Since the COVID pandemic, more teachers are familiar with videoconferencing and may even have initiated or expanded their use of asynchronous text based discussion. A hybrid combination of these platforms provides an effective, two‐fold strategy for facilitating a teacher professional learning community (PLC) that engages teachers in deepened reflection and sustained collaboration with colleagues. To illustrate how this approach can be successfully adopted, I draw from a recent example of the InquirySpace PLC that unfolded during the final year of a National Science Foundation‐funded educational research project. Key to the success of this PLC was a combination of responsiveness to teachers’ support requests, a warm and encouraging tone, orchestrating interactive engagement, and the showcasing of PLC member voices.
Metaphysical explanations: The case of singleton sets revisited
Authors: Büttner Kai Michael 
Published: February 2024

AbstractMany contemporary metaphysicians believe that the existence of a contingent object such as Socrates metaphysically explains the existence of the corresponding set {Socrates}. This paper argues that this belief is mistaken. The argument proposed takes the form of a dilemma. The expression “{Socrates}” is a shorthand either for the expression “the set that contains all and only those objects that are identical to Socrates” or for the expression “the set that contains Socrates and nothing else”. However, Socrates' existence does not explain the existence of the set that contains all and only those objects that are identical to Socrates, because there is such a set no matter whether or not Socrates exists. And although Socrates' existence does explain that of the set that contains Socrates and nothing else, this explanation is a conceptual rather than a metaphysical one. Both these claims rely on a deflationary account of the use of set theoretic vocabulary that is explained, though not properly justified, in the paper.
Comparing alternative China and the US arrangements with CPTPP
Authors: Li Chunding , Lin Xin , Whalley John 
Published: February 2024

AbstractFollowing China's formal application to join the Comprehensive and Progressive Trans‐Pacific Partnership (CPTPP), the US' intention to rejoin the trade agreement has obviously strengthened. This article develops a numerical general equilibrium model encompassing 29 countries, incorporating the concept of inside money and trade costs. The aim is to simulate and compare the impacts of China and the US participating in the CPTPP. Comparison results indicate that China entering the CPTPP would yield greater benefits for remember countries in terms of trade, GDP and manufacturing employment compared to the US. Moreover, China's participation can also positively impact GDP and manufacturing employment in most non‐member countries. Additionally, our simulation results unveil that the US would be more favoured among CPTPP members and globally concerning welfare considerations.
Comparison of plasma l‐lactate between jugular and cephalic veins in healthy and systemically ill horses using a point‐of‐care device
Authors: Rhodes Adrienne D. , Wilkins Pamela A. , McCoy Annette M. 
Published: February 2024

AbstractObjectiveTo compare plasma l‐lactate (LAC) values between samples collected from jugular and cephalic venipuncture in healthy horses and systemically ill horses.DesignProspective, experimental study.SettingLarge animal university teaching hospital.AnimalsThirty healthy adult university‐owned horses and 43 client‐owned horses presenting to the large animal hospital for elective surgical procedures or for emergent medical evaluation of systemic illness.InterventionsBlood samples were collected from the jugular vein (JV) and cephalic vein (CV) and placed in EDTA blood tubes prior to any medical therapy. LAC values were obtained with a handheld lactate meter at the time of blood collection.Measurements and Main ResultsLAC was higher in CV samples than JV samples in healthy horses (P < 0.001); however, all values were within the normal reference range. Similarly, LAC was higher in CV samples than JV samples in systemically ill horses (P < 0.001), but the median JV value was within normal reference range (1.9 mmol/L [17.1 mg/dL]), while the median CV value was outside the normal reference range (2.9 mmol/L [26.1 mg/dL]).ConclusionsThe CV is an alternative venipuncture site for assessing plasma LAC if the JV is not accessible or to preserve the JV for subsequent catheterization. However, in ill horses, the CV value may be outside the reference range when the corresponding JV value would have been within the reference range.
Routine results of an algorithm for managing the production of blood components
Authors: Pérez‐Aliaga Ana Isabel , Ayerra Irene , Sánchez‐Guillén Javier , López F. Javier , Puente Fernando , Aranda Alfonso , Domingo José María , Garcés Carmen 
Published: February 2024

AbstractBackground and ObjectivesThe variability in the number of donations together with a growing demand for platelet concentrates and plasma‐derived medicines make us seek solutions aimed at optimizing the processing of blood. Some mathematical models to improve efficiencies in blood banking have been published. The goal of this work is to validate and evaluate an algorithm's impact in the production of blood components in the Blood and Tissues Bank of Aragon (BTBA).Materials and MethodsA mathematical algorithm was designed, implemented and validated through simulations with real data. It was incorporated into the fractionation area, which uses the Reveos® fractionation system (Terumo BCT) to split blood into its components. After 9 months of daily routine validation, retrospective activity data from the Blood Bank and Transfusion Services before and during the use of the algorithm were compared.ResultsUsing the algorithm, the outdating rate of platelet concentrates (PC) decreased by 87.8% in the blood bank. The average shelf life remaining of PC supplied to Transfusion Services increased by almost 1 day. As a consequence, the outdating rate in the Aragon Transfusion Network decreased by 33%. In addition, extra 100 litres of plasma were obtained in 9 months.ConclusionsThe algorithm improves the blood establishment's workflow and facilitates the decision‐making process in whole blood processing. It resulted in a decrease in PC outdating rate, increase in PC shelf life and finally an increase in the volume of recovered plasma, leading to significant cost savings.
Experimental Investigation of a Rapid Calculation and Damage Diagnosis of the Quasistatic Influence Line of a Self-Anchored Suspension Bridge Based on Deflection Theory
Authors: Gao Qingfei , Dong Yidian , Wang Tong , Pang Baolong , Yang Shishuo 
Published: February 2024

AbstractBased on deflection theory, the basic differential equations and deformation compatibility equations of self-anchored suspension bridges are established. The calculation formula for the stiffening girder deflection of a three-span self-anchored suspension bridge is derived, and the validity of the formula is confirmed through an example. In addition, a fast calculation method for the theoretical deflection influence line is proposed by using the deflection calculation formula, and a calculation method for the actual deflection influence line is founded on the concept of time and space coordinate transformation. By loading the bridge with a load car and using real-time monitoring of the displacement response of the bridge test section, combined with the proposed intelligent damage evaluation program, the abnormal situation of the damage of the three-span self-anchored suspension bridge can be quickly and accurately identified. The research results of this paper can provide flexible, economical, and effective guidance for detecting the capacity of real bridges.
Esports Coaching: Applying a Constraints-Led Approach to Develop Team Coordination and Communication
Authors: Bubna Kabir , Trotter Michael G. , Watson Matthew 
Published: February 2024

The current case study reports on the application of a novel coaching approach in the context of a semiprofessional League of Legends team competing in Europe. The case study aimed to develop the coordinative tendencies of the team by applying a constraints-led approach that manipulated the practice environment to promote specific goal-directed behavior. The present study outlines the objectives of League of Legends, the needs analysis, intervention development, intervention delivery, and feedback from the players in regard to the effectiveness of the intervention and their development. Finally, the present study provides personal reflections from the practitioner, who was applying contemporary skill-acquisition approaches in esports for the first time.
Neddylation is essential for malaria transmission in
Plasmodium berghei
Authors: Nayak Bandita , Paul Plabita , Mishra Satish 
Published: February 2024

ABSTRACT

Neddylation is a type of posttranslational modification known to regulate a wide range of cellular processes by covalently conjugating the ubiquitin-like protein Nedd8 to target proteins at lysine residues. However, the role of neddylation in malaria parasites has not been determined. Here, for the first time, we showed that neddylation plays an essential role in malaria transmission in
Plasmodium berghei
. We found that disruption of Nedd8 did not affect blood-stage propagation, gametocyte development, gamete formation, or zygote formation while abolishing the formation of ookinetes and further transmission of the parasites in mosquitoes. These phenotypic defects in Nedd8 knockout parasites were complemented by reintroducing the gene that restored mosquito transmission to wild-type levels. Our data establish the role of
P. berghei
Nedd8 in malaria parasite transmission.

IMPORTANCE
Neddylation is a process by which Nedd8 is covalently attached to target proteins through three-step enzymatic cascades. The attachment of Nedd8 residues results in a range of diverse functions, such as cell cycle regulation, metabolism, immunity, and tumorigenesis. The potential neddylation substrates are cullin (CUL) family members, which are implicated in controlling the cell cycle. Cullin neddylation leads to the activation of cullin-RING ubiquitin ligases, which regulate a myriad of biological processes through target-specific ubiquitylation. Neddylation possibly regulates meiosis in zygotes, which subsequently develop into ookinetes. Our findings point to an essential function of this neddylation pathway and highlight its possible importance in designing novel intervention strategies.
Single-genome analysis reveals a heterogeneous association of the herpes simplex virus genome with H3K27me2 and the reader PHF20L1 following infection of human fibroblasts
Authors: Francois Alison K. , Rohani Ali , Loftus Matt , Dochnal Sara , Hrit Joel , McFarlane Steven , Whitford Abigail , Lewis Anna , Krakowiak Patryk , Boutell Chris , Rothbart Scott B. , Kashatus David , Cliffe Anna R. 
Published: February 2024

ABSTRACT

The fate of herpesvirus genomes following entry into different cell types is thought to regulate the outcome of infection. For the Herpes simplex virus 1 (HSV-1), latent infection of neurons is characterized by association with repressive heterochromatin marked with Polycomb silencing-associated lysine 27 methylation on histone H3 (H3K27me). However, whether H3K27 methylation plays a role in repressing lytic gene expression in non-neuronal cells is unclear. To address this gap in knowledge, and with consideration that the fate of the viral genome and outcome of HSV-1 infection could be heterogeneous, we developed an assay to quantify the abundance of histone modifications within single viral genome foci of infected fibroblasts. Using this approach, combined with bulk epigenetic techniques, we were unable to detect any role for H3K27me3 during HSV-1 lytic infection of fibroblasts. By contrast, we could detect the lesser studied H3K27me2 on a subpopulation of viral genomes, which was consistent with a role for H3K27 demethylases in promoting lytic gene expression. In addition, viral genomes co-localized with the H3K27me2 reader protein PHF20L1, and this association was enhanced by inhibition of the H3K27 demethylases UTX and JMJD3. Notably, targeting of H3K27me2 to viral genomes was enhanced following infection with a transcriptionally defective virus in the absence of Promyelocytic leukemia nuclear bodies. Collectively, these studies implicate a role for H3K27me2 in fibroblast-associated HSV genome silencing in a manner dependent on genome sub-nuclear localization and transcriptional activity.

IMPORTANCE
Investigating the potential mechanisms of gene silencing for DNA viruses in different cell types is important to understand the differential outcomes of infection, particularly for viruses like herpesviruses that can undergo distinct types of infection in different cell types. In addition, investigating chromatin association with viral genomes informs on the mechanisms of epigenetic regulation of DNA processes. However, there is a growing appreciation for heterogeneity in the outcome of infection at the single cell, and even single viral genome, level. Here we describe a novel assay for quantifying viral genome foci with chromatin proteins and show that a portion of genomes are targeted for silencing by H3K27me2 and associate with the reader protein PHF20L1. This study raises important questions regarding the mechanism of H3K27me2-specific targeting to viral genomes, the contribution of epigenetic heterogeneity to herpesvirus infection, and the role of PHF20L1 in regulating the outcome of DNA virus infection.
Lake Erie ice is a repository of organisms
Authors: Iwaloye Opeoluwa F. , Michaud Brenna , Alloy Tessa , D'Souza Nigel , McKay R. Michael L. , Morris Paul , Gura Colby , Rogers Scott O. 
Published: February 2024

ABSTRACT

Organism abundance and diversity were assessed in Lake Erie ice samples using sequences derived from a combined metagenomic and metatranscriptomic analysis. The 68,417 unique sequences were from Bacteria (77.5%), Eukarya (22.3%), and Archaea (0.2%) and indicated diverse species of organisms from 32 bacterial, 8 eukaryotic, and 2 archaeal taxonomic groups.
Whole-genome sequencing of SARS-CoV-2 from the initial cases of domestic cat infections in Canada
Authors: Sultana Asma , Bienzle Dorothee , Weese Scott , Pickering Brad , Kruczkiewicz Peter , Smith Greg , Pinette Mathieu , Lung Oliver 
Published: February 2024

ABSTRACT

Two cat nasal swabs from Canada’s earliest confirmed SARS-CoV-2 positive domestic cats were sequenced to over 99% SARS-CoV-2 genome coverage. One cat had lineage A.23.1 SARS-CoV-2 not reported before in animals. Both sequences have multiple spike gene mutations and clustered closely with human-derived sequences in the global SARS-CoV-2 phylogenetic tree.
Genomic revisitation and reclassification of the genus
Providencia
Authors: Dong Xu , Jia Huiqiong , Yu Yuyun , Xiang Yanghui , Zhang Ying 
Published: February 2024

ABSTRACT

Members of
Providencia
, although typically opportunistic, can cause severe infections in immunocompromised hosts. Recent advances in genome sequencing provide an opportunity for more precise study of this genus. In this study, we first identified and characterized a novel species named
Providencia zhijiangensis
sp. nov. It has ≤88.23% average nucleotide identity (ANI) and ≤31.8%
in silico
DNA-DNA hybridization (dDDH) values with all known
Providencia
species, which fall significantly below the species-defining thresholds. Interestingly, we found that
Providencia stuartii
and
Providencia thailandensis
actually fall under the same species, evidenced by an ANI of 98.59% and a dDDH value of 90.4%. By fusing ANI with phylogeny, we have reclassified 545 genomes within this genus into 20 species, including seven unnamed taxa (provisionally titled Taxon 1–7), which can be further subdivided into 23 lineages. Pangenomic analysis identified 1,550 genus-core genes in
Providencia
, with coenzymes being the predominant category at 10.56%, suggesting significant intermediate metabolism activity. Resistance analysis revealed that most lineages of the genus (82.61%, 19/23) carry a high number of antibiotic-resistance genes (ARGs) and display diverse resistance profiles. Notably, the majority of ARGs are located on plasmids, underscoring the significant role of plasmids in the resistance evolution within this genus. Three species or lineages (
P. stuartii
, Taxon 3, and
Providencia hangzhouensis
L12) that possess the highest number of carbapenem-resistance genes suggest their potential influence on clinical treatment. These findings underscore the need for continued surveillance and study of this genus, particularly due to their role in harboring antibiotic-resistance genes.

IMPORTANCE

The
Providencia
genus, known to harbor opportunistic pathogens, has been a subject of interest due to its potential to cause severe infections, particularly in vulnerable individuals. Our research offers groundbreaking insights into this genus, unveiling a novel species,
Providencia zhijiangensis
sp. nov., and highlighting the need for a re-evaluation of existing classifications. Our comprehensive genomic assessment offers a detailed classification of 545 genomes into distinct species and lineages, revealing the rich biodiversity and intricate species diversity within the genus. The substantial presence of antibiotic-resistance genes in the
Providencia
genus underscores potential challenges for public health and clinical treatments. Our study highlights the pressing need for increased surveillance and research, enriching our understanding of antibiotic resistance in this realm.
Sleep quality and its associated factors among patients with type 2 diabetes mellitus in Hunan, China: a cross-sectional study
Authors: Maimaitituerxun Rehanguli , Chen Wenhang , Xiang Jingsha , Xie Yu , Xiao Fang , Wu Xin Yin , Chen Letao , Yang Jianzhou , Liu Aizhong , Dai Wenjie 
Published: February 2024

ObjectivesType 2 diabetes mellitus (T2DM) is a serious public health issue. Compared with the general population, patients with T2DM have a higher risk of poor sleep quality, which could ultimately result in poor prognosis. Therefore, this study aimed to evaluate sleep quality and its associated factors among patients with T2DM in Hunan, China.DesignThis was a cross-sectional study.SettingA tertiary hospital in Hunan, China.ParticipantsPatients with T2DM hospitalised at the Endocrinology Department were consecutively enrolled between March 2021 and December 2022. Sociodemographic characteristics, lifestyle factors and T2DM-related information were collected retrospectively.Primary and secondary outcome measuresSleep quality was evaluated using the Pittsburgh Sleep Quality Index, with a cut-off value of >7 suggesting poor sleep quality. Multivariate logistic regression analysis was used to determine factors associated with poor sleep quality.ResultsOf the 1039 participants included, 1001 provided complete data. The mean age of the study sample was 60.24±10.09 years, and 40.5% (95% CI 37.5% to 43.5%) of patients had poor sleep quality. Multivariate logistic regression analysis showed that female sex (adjusted OR (aOR) 1.70, 95% CI 1.25 to 2.29), unmarried status (aOR 1.72, 95% CI 1.05 to 2.83), diabetic retinopathy (aOR 1.38, 95% CI 1.04 to 1.83), diabetic foot (aOR 1.80, 95% CI 1.11 to 2.93) and a per capita monthly household income of >5000 RMB (aOR 0.66, 95% CI 0.47 to 0.93) were associated with poor sleep quality.ConclusionsNearly two-fifths of patients with T2DM reported poor sleep quality in Hunan, China. Sex, marital status, diabetic retinopathy, diabetic foot and household income were independently associated with sleep quality among patients with T2DM in Hunan, China.
Demonstration of particle tracking with scintillating fibres read out by a SPAD array sensor and application as a neutrino active target
Authors: Franks Matthew , Dieminger Till , Kaneyasu Kodai , Sgalaberna Davide , Bruschini Claudio , Charbon Edoardo , Kose Umut , Li Botao , Mos Paul , Wayne Michael , Weber Tim , Wu Jialin 
Published: February 2024

AbstractScintillating fibre detectors combine sub-mm resolution particle tracking, precise measurements of the particle stopping power and sub-ns time resolution. Typically, fibres are read out with silicon photomultipliers (SiPM). Hence, if fibres with a few hundred $$\upmu $$
μ
m diameter are used, either they are grouped together and coupled with a single SiPM, losing spatial resolution, or a very large number of electronic channels is required. In this article we propose and provide a first demonstration of a novel configuration which allows each individual scintillating fibre to be read out regardless of the size of its diameter, by imaging them with Single-Photon Avalanche Diode (SPAD) array sensors. Differently from SiPMs, SPAD array sensors provide single-photon detection with single-pixel spatial resolution. In addition, O(us) or faster coincidence of detected photons allows to obtain noise-free images. Such a concept can be particularly advantageous if adopted as a neutrino active target, where scintillating fibres alternated along orthogonal directions can provide isotropic, high-resolution tracking in a dense material and reconstruct the kinematics of low-momentum protons (down to 150 MeV/c), crucial for an accurate characterisation of the neutrino-nucleus cross section. In this work the tracking capabilities of a bundle of scintillating fibres coupled to SwissSPAD2 is demonstrated. The impact of such detector configuration in GeV-neutrino experiments is studied with simulations and reported. Finally, future plans, including the development of a new SPAD array sensor optimised for neutrino detection, are discussed.
A Note to Non-adaptive Broadcasting
Authors: Gholami Saber , Harutyunyan Hovhannes A. 
Published: February 2024

Broadcasting is a fundamental problem in the information dissemination area. In classical broadcasting, a message must be sent from one network member to all other members as rapidly as feasible. Although it has been demonstrated that this problem is NP-Hard for arbitrary graphs, it has several applications in various fields. As a result, the universal lists model, replicating real-world restrictions like the memory limits of nodes in large networks, is introduced as a branch of this problem in the literature. In the universal lists model, each node is equipped with a fixed list and has to follow the list regardless of the originator. In this study, we focus on the non-adaptive branch of universal lists broadcasting. In this regard, we establish the optimal broadcast time of [Formula: see text]-ary trees and binomial trees under the non-adaptive model and provide an upper bound for complete bipartite graphs. We also improved a general upper bound for trees under the same model and showed that our upper bound cannot be improved in general.