bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D printing of gellan‐dextran methacrylate IPNs in glycerol and their bioadhesion by RGD derivatives
Authors: Paoletti Luca , Baschieri Francesco , Migliorini Claudia , Di Meo Chiara , Monasson Olivier , Peroni Elisa , Matricardi Pietro 
Published: March 2024

AbstractThe ever‐growing need for new tissue and organ replacement approaches paved the way for tissue engineering. Successful tissue regeneration requires an appropriate scaffold, which allows cell adhesion and provides mechanical support during tissue repair. In this light, an interpenetrating polymer network (IPN) system based on biocompatible polysaccharides, dextran (Dex) and gellan (Ge), was designed and proposed as a surface that facilitates cell adhesion in tissue engineering applications. The new matrix was developed in glycerol, an unconventional solvent, before the chemical functionalization of the polymer backbone, which provides the system with enhanced properties, such as increased stiffness and bioadhesiveness. Dex was modified introducing methacrylic groups, which are known to be sensitive to UV light. At the same time, Ge was functionalized with RGD moieties, known as promoters for cell adhesion. The printability of the systems was evaluated by exploiting the ability of glycerol to act as a co‐initiator in the process, speeding up the kinetics of crosslinking. Following semi‐IPNs formation, the solvent was removed by extensive solvent exchange with HEPES and CaCl2, leading to conversion into IPNs due to the ionic gelation of Ge chains. Mechanical properties were investigated and IPNs ability to promote osteoblasts adhesion was evaluated on thin‐layer, 3D‐printed disk films. Our results show a significant increase in adhesion on hydrogels decorated with RGD moieties, where osteoblasts adopted the spindle‐shaped morphology typical of adherent mesenchymal cells. Our findings support the use of RGD‐decorated Ge/Dex IPNs as new matrices able to support and facilitate cell adhesion in the perspective of bone tissue regeneration.
Mineral matrix deposition of MC3T3‐E1 pre‐osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite
Authors: Rincón‐López July Andrea , Hermann‐Muñoz Jennifer Andrea , Detsch Rainer , Rangel‐López Raúl , Muñoz‐Saldaña Juan , Jiménez‐Sandoval Sergio , Alvarado‐Orozco Juan Manuel , Boccaccini Aldo R. 
Published: March 2024

AbstractThis work presents the effect of the silicocarnotite (SC) and nagelschmidtite (Nagel) phases on in vitro osteogenesis. The known hydroxyapatite of biological origin (BHAp) was used as a standard of osteoconductive characteristics. The evaluation was carried out in conventional and osteogenic media for comparative purposes to assess the osteogenic ability of the bioceramics. First, the effect of the material on cell viability at 24 h, 7 and 14 days of incubation was evaluated. In addition, cell morphology and attachment on dense bioceramic surfaces were observed by fluorescence microscopy. Specifically, alkaline phosphatase (ALP) activity was evaluated as an osteogenic marker of the early stages of bone cell differentiation. Mineralized extracellular matrix was observed by calcium phosphate deposits and extracellular vesicle formation. Furthermore, cell phenotype determination was confirmed by scanning electron microscope. The results provided relevant information on the cell attachment, proliferation, and osteogenic differentiation processes after 7 and 14 days of incubation. Finally, it was demonstrated that SC and Nagel phases promote cell proliferation and differentiation, while the Nagel phase exhibited a superior osteoconductive behavior and could promote MC3T3‐E1 cell differentiation to a higher extent than SC and BHAp, which was reflected in a higher number of deposits in a shorter period for both conventional and osteogenic media.
Study of absorption and emission spectra of substituted terthiophene compounds by methods of theoretical chemistry
Authors: Kužela Tomáš , Bondarev Dmitrij , Kutálková Eva , Benková Zuzana , Hrnčiřík Josef , Ingr Marek 
Published: March 2024

AbstractTerthiophene derivatives attract interest due to their prospective applications in optoelectronic or sensor devices. Due to their nontoxicity they can be considered as suitable candidates in biological applications. Supramolecular organization of the matter is one of the most interesting topics in contemporary materials science. Amphiphilic chromophores based on substituted terthiophenes are capable of self‐assembly into supramolecular architectures. In this work, we aim at simulation of the spectral properties of terthiophene with oligo(ethylene oxide) substituents by the methods of quantum chemistry and molecular dynamics (MD). The potential energy surface (PES) of this molecule was determined by the methods of density functional theory (DFT) for the ground state and time‐dependent density‐functional theory (TD‐DFT) for the excited state. MD simulations in water than revealed the most frequented molecular conformations in both these states. Absorption and fluorescence spectra were determined for all these conformations, including the surrounding water molecules, using TD‐DFT and averaged over the conformation space to obtain the final absorption and fluorescence spectrum. The calculated spectra were compared with their experimental counterparts and the differences were discussed in context of the supramolecular structure revealed by confocal microscopy. In spite of its simplicity, this approach provides a satisfactory approximation of absorption and fluorescent spectra of these molecules obtained by computational methods.
Pharmacokinetic and Pharmacodynamic Modeling Analysis of Delpazolid (LCB01‐0371) in Adult Patients with Pulmonary Tuberculosis
Authors: Lee Sang Min , Choi Seung Chan , Mun Kyung Ran , Seo Ji Young , Cho Young Lag , Shim Tae Sun , Lim Hyeong‐Seok 
Published: March 2024

AbstractDelpazolid (LCB01‐0371) is a novel oxazolidinone derivative with a good safety profile for treating gram‐positive pathogenic infections such as Mycobacterium abscessus, a highly pathogenic drug‐resistant Mycobacterium. In this study, we evaluated the pharmacokinetics (PK) and pharmacodynamics (PD) of delpazolid after 14 days of multiple oral administration, using data from adult patients with pulmonary tuberculosis. 800 mg once a day, 400 mg twice a day, 800 mg twice a day, and 1200 mg once a day delpazolid for 14 days were tested in 63 patients with pulmonary tuberculosis. For PK blood collection, inpatient and outpatient scheduling were separately implemented. Plasma concentrations of delpazolid were measured at visits 2, 4, 6, and 8 in outpatients, and four sparse blood samples were measured in inpatients. PD models were sequentially fitted using individual PK parameter estimates obtained from PK compartmental models. For PK modeling, 180 plasma concentrations of delpazolid from 56 patients were included. A two‐compartment mixed first‐ and zero‐order absorption model best described the time course of plasma concentration. For the PD model, 448 bacterial titer data from 60 patients were used. The time course of bacterial titers (log10 CFU/mL) was described by a model that consists of the growth and killing rate of bacteria with the sigmoid Emax model. The PK–PD simulation suggested that the bacterial titers are the lowest on the 800 mg bid regimen among the four, consistent with observed data, as all regimens substantially decrease. In the dose–response relationship, the effectiveness of delpazolid was suggested.
Mechanobiology of bacterial biofilms: Implications for orthopedic infection
Authors: Blondel Margaux , Machet Camille , Wildemann Britt , Abidine Yara , Swider Pascal 
Published: March 2024

AbstractPostoperative bacterial infections are prevalent complications in both human and veterinary orthopedic surgery, particularly when a biofilm develops. These infections often result in delayed healing, early revision, permanent functional loss, and, in severe cases, amputation. The diagnosis and treatment pose significant challenges, and bacterial biofilm further amplifies the therapeutic difficulty as it confers protection against the host immune system and against antibiotics which are usually administered as a first‐line therapeutic option. However, the inappropriate use of antibiotics has led to the emergence of numerous multidrug‐resistant organisms, which largely compromise the already imperfect treatment efficiency. In this context, the study of bacterial biofilm formation allows to better target antibiotic use and to evaluate alternative therapeutic strategies. Exploration of the roles played by mechanical factors on biofilm development is of particular interest, especially because cartilage and bone tissues are reactive environments that are subjected to mechanical load. This review delves into the current landscape of biofilm mechanobiology, exploring the role of mechanical factors on biofilm development through a multiscale prism starting from bacterial microscopic scale to reach biofilm mesoscopic size and finally the macroscopic scale of the fracture site or bone–implant interface.
Chronology of drill cores and the inferred coastal environmental evolution on Haitan Island, South China
Authors: Qiu Junjie , Jin Jianhui , Zuo Xinxin , Fan Xuechun , Wei Junjie , Xu Daiyu , Hou Chenyang 
Published: March 2024

ABSTRACTSoutheastern China's coastal region is considered one of the key areas for studying prehistoric human–land relationships in the Western Pacific region. This region, which is rich in records of marine civilization, provides an ideal location for recording coastal environmental evolution. In this study, the chronological framework of three borehole cores recovered from Haitan Island, South China, near the Keqiutou group of sites, was established using both optically stimulated luminescence (OSL) and accelerator mass spectrometry radiocarbon (AMS 14C) dating techniques. Various analyses, including lithologic characteristics, chroma, clay–water electric conductivity (EC), grain size and elemental geochemistry, were conducted to elaborate the sedimentary sequence and its paleoenvironmental implications. The findings suggest that (i) since the late Quaternary, the Haitan Island sedimentary sequence can be divided into three deposition stages: Old Red Sand formed during Marine Isotope Stage 3 (MIS3), intermittent sedimentation during the Last Glacial Maximum and coastal aeolian deposition during the Holocene. Furthermore, the study reveals that terrestrial debris was significant as a material source for Haitan Island. (ii) Due to the absence of tectonic uplift, Haitan Island has no thick marine sedimentary record during the Holocene period of high sea level. The Luyangpu coastal sand sheet can be dated to 2.78 ka, which is consistent with previous reports giving dates of 2.4 ka. Prior to the formation of the sand sheet, the sedimentary environment in the area was characterized by lagoon or marsh facies. (iii) Paleoanthropological activities were influenced by sedimentary environmental evolution and geomorphic processes, among others. The Keqiutou Culture experienced high sea levels, which created an intertidal environment near the site and facilitated fishing. However, regression at 5.7 ka led to terrestrial environments, which prompted the inhabitants to migrate. Approximately 3 ka ago, sea levels rose again, coinciding with the initiation of rice cultivation. These factors may have influenced the transition from the Neolithic to Bronze Age culture. This study reconstructs evolution of the sedimentary sequence since the late Quaternary, enhancing our understanding of past human–terrestrial relationship in the region.
Evolution of fermented grain yeast communities in strong‐flavored baijiu and functional validation of yeasts that produce superior‐flavored substances
Authors: Duan Zhong‐Fu , Han Mei‐Yue , Niu Jia‐Liang , Zhao Jing‐Rong , Li Wei‐Wei , Zhu Li‐Ning , Ma Hui‐Feng , Wu Yan‐Fang , Li Xiu‐Ting , Sun Bao‐Guo 
Published: March 2024

AbstractBACKGROUNDBaijiu is a well‐known alcoholic beverage in China and the quality is determined by various microorganisms during the fermentation process. Yeast is one of the most important microorganisms in the fermentation of baijiu. It has a strong esterification capacity and also affects the aroma.RESULTSHigh‐throughput sequencing results showed that the fermented grains (jiupei) during baijiu production were mainly composed of eight highly abundant yeast species. The species and abundance of yeasts changed significantly with the fermentation process. The flavor of 30 yeast strains in the jiupei was determined by a sniffing test and gas chromatography–mass spectrometry (GC–MS). The strain with the highest flavor substance content (2.34 mg L−1), named YX3205, was identified as Clavispora lusitaniae. Tolerance results showed that C. lusitaniae YX3205 can tolerate up to 15% (v v−1) ethanol. In a solid‐state simulated fermentation experiment, the content of 24 flavor substances was significantly increased in the fortified group, and the total ester content reached 4240.73 μg kg‐1, which was 2.8 times higher than that of the control group.CONCLUSIONThe present study demonstrated the potential of C. lusitaniae YX3205 to enhance the flavor of baijiu, thereby serving as a valuable strain for the improvement of the flavor quality of baijiu. © 2024 Society of Chemical Industry.
Behavior of diclofenac from contaminated fish after cooking and in vitro digestion
Authors: Mello Flávia V. , Marmelo Isa , Fogaça Fabíola H.S. , Déniz Fernando Lafont , Alonso Mariana B. , Maulvault Ana Luísa , Torres João Paulo M. , Marques Antônio , Fernandes José O. , Cunha Sara C. 
Published: March 2024

AbstractBACKGROUNDSeafood consumers are widely exposed to diclofenac due to the high contamination levels often present in aquatic organisms. It is a potential risk to public health due its endocrine disruptor properties. Limited information is available about diclofenac behavior after food digestion to enable a more realistic scenario of consumer exposure. This study aimed to evaluate cooking effects on diclofenac levels, and determine diclofenac bioaccessibility by an in vitro digestion assay, using commercial fish species (seabass and white mullet) as models. The production of the main metabolite 4′‐hydroxydiclofenac was also investigated. Fish hamburgers were spiked at two levels (150 and 1000 ng g−1) and submitted to three culinary treatments (roasting, steaming and grilling).RESULTSThe loss of water seems to increase the diclofenac levels after cooking, except in seabass with higher levels. The high bioaccessibility of diclofenac (59.1–98.3%) observed in both fish species indicates that consumers' intestines are more susceptible to absorption, which can be worrisome depending on the level of contamination. Contamination levels did not affect the diclofenac bioaccessibility in both species. Seabass, the fattest species, exhibited a higher bioaccessibility of diclofenac compared to white mullet. Overall, cooking decreased diclofenac bioaccessibility by up to 40% in seabass and 25% in white mullet. The main metabolite 4′‐hydroxydiclofenac was not detected after cooking or digestion.CONCLUSIONThus, consumption of cooked fish, preferentially grilled seabass and steamed or baked white mullet are more advisable. This study highlights the importance to consider bioaccessibility and cooking in hazard characterization studies. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Two‐view autostereoscopic display independent of differences of interocular distance and viewing condition
Authors: Hori Takumi , Yoshimoto Kayo , Hamagishi Goro , Takahashi Hideya 
Published: March 2024

AbstractWe have previously proposed the parallax barrier autostereoscopic display with eye tracking control. This display realized a wide viewing area. However, the previous method simplifies the process by using the average interocular distance and composing images corresponding to the center position of both eyes. As a result, crosstalk caused by individual differences in interocular distance and changes in viewing condition, such as facial tilt and rotation, was occurred. The crosstalk caused by individual differences in interocular distance is an important factor that must be eliminated. Therefore, we propose the method to compose a parallax image by using positions of both eyes to expand the viewing zone. We use the black images to the crosstalk subpixels, which can be determined from the positions of each eye and achieves more comfortable stereoscopic viewing. To verify the effectiveness of the proposed method, we constructed the prototype 3D display using the parallax barrier with 50% aperture ratio and confirmed the wide viewing area with the crosstalk ratio of less than 5%. We can achieve an autostereoscopic display that has bright and high‐quality stereoscopic image and independent of individual differences in interocular distance and changes in viewing condition.
Enabling knowledge sharing in a production context in China
Authors: Muniz Jorge , Ribeiro Vagner Batista , Hong Jacky Fok Loi , Wintersberger Daniel , Salomon Valerio Antonio Pamplona 
Published: March 2024

AbstractThere is limited research on the cultural influence on knowledge sharing in production contexts. In view of the gap in literature, this study draws on a multi‐criteria decision‐making approach, the analytic hierarchy process (AHP), to identify and rank different enabling factors of knowledge sharing in the workplace of a Chinese electronics plant on the basis of the relative importance ascribed to them by operators, leaders, and managers. The fieldwork research was conducted in a Chinese electronic plant, considering the judgment of 121 operators (blue‐collar workers), 56 production leaders, and 9 managers selected by convenience. The findings reinforce the understanding about the importance of integrating factors related to people, process, and knowledge to enable knowledge management in different cultural contexts, which may support managers' needs for lessons learned from other organizations in the areas of knowledge management, organizational learning, and process management.
Up to date diagnosis and treatment of massive irreparable cuff tears: Results of the survey of the U45 ESSKA Committee
Authors: Cerciello Simone , Ollivier Matthieu , Giordano Matthew Charles , Kocaoglu Baris ,   The ESSKA U45 Committee
Published: March 2024

AbstractPurposeIrreparable rotator cuff tears are disabling and can severely affect daily life activities, especially when young and active patients are involved. The definition is still debated, and they can have several clinical presentations. The treatment depends on the clinical presentation and the patient's age.MethodsThe survey was developed by the ESSKA U45 Committee and was uploaded on the ESSKA website. Fifty‐seven questionnaires were returned. Several aspects of the diagnosis and treatment of massive irreparable cuff tears were investigated. Moreover, treatment options for specific clinical scenarios were given for posterior‐superior and anterior‐superior cuff tears in young and old patients.ResultsFatty degeneration of the muscles was the most common criterion to define an irreparable tear (59.7%). In young patients with external rotation with the arm at side lag, partial repair of the cuff was the most common option (41.8%); in case of external rotation with the arm at side + external rotation at 90° of abduction lag without pseudoparalysis, the most common option was partial repair of the cuff + latissimus dorsi or lower trapezius transfer (39.3%), and in case of external rotation with the arm at side + external rotation at 90° of abduction lag with pseudoparalysis, partial repair of the cuff + latissimus dorsi or lower trapezius transfer was still the most common option (25.5%). The same scenarios in old patients yielded the following results: reverse prosthesis (49.1%), reverse prosthesis (44.6%) and reverse prosthesis ± latissimus dorsi or lower trapezius transfer (44.6%), respectively.ConclusionsThe present survey clearly confirms that biological options (partial cuff repairs and tendon transfers) are the reference in the case of young patients with deficient cuffs (both posterior and anterior). Reverse shoulder prosthesis is the most common treatment option in old patients in all clinical scenarios.Level of EvidenceLevel IV.
Quantification and Functional Studies of Neutrophilic Cells Identifies Distinct Papilloma Phenotypes
Authors: Bai Ke , Clavijo Paul E. , Robbins Yvette , Norberg Scott M. , Allen Clint T. 
Published: March 2024

ObjectivesTo characterize the distribution of immune cell subsets within laryngeal papillomas and to study the function of potentially immunosuppressive neutrophilic and regulatory T cells (Tregs).MethodsFresh clinical papilloma specimens were collected at the time of surgery and studied with multiparameter flow cytometry. Papilloma infiltrating neutrophilic cells and Tregs were sorted and studied functionally with ex vivo T cell suppression assays.ResultsFlow cytometric analysis of fresh laryngeal papillomas samples from 18 adult patients with recurrent respiratory papillomatosis revealed patterns in immune constituency between patients. Clearly divergent phenotypes based primarily on the degree of neutrophilic and T cell infiltration were identified. Relative neutrophilic cell enrichment and T cell depletion were observed in 50% of samples and neutrophilic cell depletion and T cell enrichment were observed in the others. Greater papilloma neutrophilic cell enrichment was positively associated with the number of clinically indicated interventions required in the 12 months prior to sample collection, linking papilloma neutrophil inflammation to disease severity. Functional assays revealed the ability of both papilloma infiltrating neutrophilic and Tregs to suppress T cell function at roughly equal magnitudes, but substantially increased infiltration of neutrophilic cells compared to Tregs across samples.ConclusionNeutrophilic cells are an important contributor to immunosuppression within the respiratory papilloma microenvironment. Given these data and the association between greater neutrophilic cell infiltration and lack of clinical response to therapeutic vaccination, additional study of strategies aimed at limiting neutrophilic cell infiltration or function within papillomas is warranted.Level of Evidence4 Laryngoscope, 134:3238–3244, 2024
The effect of SMAW welding parameters on stress corrosion cracking of the X65 gas pipelines steel
Authors: Nooriun Hanie , Hamidi Ahmad Ghaderi , Momeni Amir 
Published: March 2024

AbstractSteel pipelines used for oil and gas transmission are at risk of stress corrosion cracking (SCC). Extensive research has been carried out to evaluate the SCC susceptibility of pipeline steels. Those research studies were mainly specific to the steels of pipes without welding. In this research, pieces of X65 steel plates were welded by the shielded metal arc welding process. The speeds of electrode travel were set as the main welding parameter. Slow strain rate tests (SSRTs) in the air and the NS4 standard solution were conducted. The results showed that the tensile behavior of the samples in the solution was very different from that in the air. For the samples tested in the solution, the strength amounts of the weld zones were higher than the strength of the base metal, but in the case of SSRT in the air, the strength of the base metal was higher. As a result, the UTS strength of one of the weld zones reached levels as high as 480 MPa and also 34% elongation under the SSRT. Therefore, the conditions of welding have a high degree of importance and must be controlled to have less SCC susceptibility.