bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility of an MR-based digital specimen for tongue cancer resection specimens: a novel approach for margin evaluation
Authors: de Koning Klijs Jacob , Dankbaar Jan Willem , de Keizer Bart , Willemsen Koen , van der Toorn Annette , Breimer Gerben Eise , van Es Robert Jelle Johan , de Bree Remco , Noorlag Rob , Philippens Marielle Emile Petronella 
Published: March 2024

ObjectiveThis study explores the feasibility of ex-vivo high-field magnetic resonance (MR) imaging to create digital a three-dimensional (3D) representations of tongue cancer specimens, referred to as the “MR-based digital specimen” (MR-DS). The aim was to create a method to assist surgeons in identifying and localizing inadequate resection margins during surgery, a critical factor in achieving locoregional control.MethodsFresh resection specimens of nine tongue cancer patients were imaged in a 7 Tesla small-bore MR, using a high-resolution multislice and 3D T2-weighted Turbo Spin Echo. Two independent radiologists (R1 and R2) outlined the tumor and mucosa on the MR-images whereafter the outlines were configured to an MR-DS. A color map was projected on the MR-DS, mapping the inadequate margins according to R1 and R2. We compared the hematoxylin-eosin-based digital specimen (HE-DS), which is a histopathological 3D representation derived from HE stained sections, with its corresponding MR-images. In line with conventional histopathological assessment, all digital specimens were divided into five anatomical regions (anterior, posterior, craniomedial, caudolateral and deep central). Over- and underestimation 95th-percentile Hausdorff-distances were calculated between the radiologist- and histopathologist-determined tumor outlines. The MR-DS’ diagnostic accuracy for inadequate margin detection (i.e. sensitivity and specificity) was determined in two ways: with conventional histopathology and HE-DS as reference.ResultsUsing conventional histopathology as a reference, R1 achieved 77% sensitivity and 50% specificity, while R2 achieved 65% sensitivity and 57% specificity. When referencing to the HE-DS, R1 achieved 94% sensitivity and 61% specificity, while R2 achieved 88% sensitivity and 71% specificity. Range of over- and underestimation 95HD was 0.9 mm - 11.8 mm and 0.0 mm - 5.3 mm, respectively.ConclusionThis proof of concept for volumetric assessment of resection margins using MR-DSs, demonstrates promising potential for further development. Overall, sensitivity is higher than specificity for inadequate margin detection, because of the radiologist’s tendency to overestimate tumor size.
Early life phenobarbital exposure dysregulates the hippocampal transcriptome
Authors: Quinlan Seán , Khan Tahiyana , McFall David , Campos-Rodriguez Carolina , Forcelli Patrick A. 
Published: March 2024

Introduction: Phenobarbital (PB) and levetiracetam (LEV) are the first-line therapies for neonates with diagnosed seizures, however, a growing body of evidence shows that these drugs given during critical developmental windows trigger lasting molecular changes in the brain. While the targets and mechanism of action of these drugs are well understood-what is not known is how these drugs alter the transcriptomic landscape, and therefore molecular profile/gene expression during these critical windows of neurodevelopment. PB is associated with a range of neurotoxic effects in developing animals, from cell death to altered synaptic development to lasting behavioral impairment. LEV does not produce these effects.Methods: Here we evaluated the effects of PB and Lev on the hippocampal transcriptome by RNA sequencing. Neonatal rat pups were given a single dose of PB, Lev or vehicle and sacrificed 72 h later-at time at which drug is expected to be cleared.Results: We found PB induces broad changes in the transcriptomic profile (124 differentially expressed transcripts), as compared to relatively small changes in LEV-treated animals (15 transcripts). PB exposure decreased GABAergic and oligodendrocyte markers pvalb and opalin, and increased the marker of activated microglia, cd68 and the astrocyte- associated gene vegfa. These data are consistent with the existing literature showing developmental neurotoxicity associated with PB, but not LEV.Discussion: The widespread change in gene expression after PB, which affected transcripts reflective of multiple cell types, may provide a link between acute drug administration and lasting drug toxicity.
Gut microbes combined with metabolomics reveal the protective effects of Qijia Rougan decoction against CCl4-induced hepatic fibrosis
Authors: Li Xue , Xu Xinyi , Tao Sian , Su Yue , Wen Li , Wang Dong , Liu Jibin , Feng Quansheng 
Published: March 2024

Background: The occurrence and development of Hepatic fibrosis (HF) are closely related to the gut microbial composition and alterations in host metabolism. Qijia Rougan decoction (QJ) is a traditional Chinese medicine compound utilized clinically for the treatment of HF with remarkable clinical efficacy. However, its effect on the gut microbiota and metabolite alterations is unknown. Therefore, our objective was to examine the impact of QJ on the gut microbiota and metabolism in Carbon tetrachloride (CCl4)-induced HF.Methods: 40% CCl4 was used to induce HF, followed by QJ administration for 6 weeks. Serum biochemical analyses, histopathology, immunohistochemistry, RT-PCR, 16S rRNA gene sequencing, and non-targeted metabolomics techniques were employed in this study to investigate the interventional effects of QJ on a CCl4-induced HF model in rats.Results: This study demonstrated that QJ could effectively ameliorate CCl4-induced hepatic inflammation and fibrosis. Moreover, QJ upregulated the expression of intestinal tight junction proteins (TJPs) and notably altered the abundance of some gut microbes, for example, 10 genera closely associated with HF-related indicators and TJPs. In addition, metabolomics found 37 key metabolites responded to QJ treatment and strongly associated with HF-related indices and TJPs. Furthermore, a tight relation between 10 genera and 37 metabolites was found post correlation analysis. Among them, Turicibacter, Faecalibaculum, Prevotellaceae UCG 001, and unclassified Peptococcaceae may serve as the core gut microbes of QJ that inhibit HF.Conclusion: These results suggest that QJ ameliorates hepatic inflammation and fibrosis, which may be achieved by improving intestinal tight junctions and modulating gut microbiota composition as well as modulating host metabolism.
Response of cotton growth, yield, and water and nitrogen use efficiency to nitrogen application rate and ionized brackish water irrigation under film-mulched drip fertigation
Authors: Wei Kai , Wang Quanjiu , Deng Mingjiang , Lin Shudong , Guo Yi 
Published: March 2024

IntroductionThe presence of brackish water resources is significant in addressing the scarcity of freshwater resources, particularly in the Xinjiang region. Studies focused on reducing adverse effect of brackish water irrigation based on using ionized brackish water, as well as on investigating its effects on fibre and oil plant production processes, remain incipient in the literature. Some benefits of this technique are the optimization of the quality and quantity of irrigation water, economy of water absorbed by the plants, improvement in the vegetative growth and productivity compared to irrigation using conventional brackish water. Thus, the aim of the current study is to assess the effect of different nitrogen application rates on soil water and salinity, cotton growth and water and nitrogen use efficiency.MethodsThe experimental design consisted of completely randomized design with two water types (ionized and non-ionized) and six nitrogen application rates with four replications.ResultsIrrigation conducted with ionized brackish water and different nitrogen application rates had significant effect on the plant height, leaf area index, shoot dry matter, boll number per plant and chlorophyll content. The study also demonstrated significant effects of ionized brackish water on soil water content and soil salinity accumulation. The highest cotton production was achieved with the use of 350 kg·ha-1 of ionized brackish water for irrigation, resulting in an average increase of 11.5% compared to the use of non-ionized brackish water. The nitrogen application exhibits a quadratic relationship with nitrogen agronomic use efficiency and apparent nitrogen use efficiency, while it shows a liner relationship with nitrogen physiological use efficiency and nitrogen partial productivity. After taking into account soil salinity, cotton yield, water and nitrogen use efficiency, the optimal nitrogen application rate for ionized brackish water was determined to be 300 kg·ha-1.DiscussionIt is hoped that this study can contribute to improving water management, reducing the environmental impact without implying great costs for the producer.
Does presenting perpetrator and innocent suspect faces from different facial angles influence the susceptibility of eyewitness memory? An investigation into the misinformation effect and eyewitness misidentification
Authors: Deering Kara , Colloff Melissa F. , Bennett Tia C. , Flowe Heather D. 
Published: March 2024

IntroductionThis study investigated the effects of face angle congruency across stages of a misinformation paradigm on lineup discrimination accuracy.MethodsIn a between-subjects design, participants viewed a mock crime with the perpetrator’s face from the front or profile angle. They then read a news report featuring an innocent suspect’s image from the same or different angle as the perpetrator had been shown. A subsequent lineup manipulated perpetrator presence and viewing angle of the lineup members, who were all shown either from the front or in profile.ResultsNo significant difference emerged in identification errors based on angle congruency between stages. However, accuracy was higher when faces were shown from the front angle, both during the initial event and the lineup, compared to the profile angle.DiscussionThe results of this research underscore the importance of considering viewing angles in the construction of lineups.
How to form shared objects to enhance university–school collaboration? A cultural–historical activity theory perspective
Authors: Fang Xueqin , Mao Qiming , Hong Jianzhong Janne , Diao Chunting 
Published: March 2024

IntroductionUniversity-school (U–S) collaboration has proven to be an effective approach for teacher professional development, but it could be hampered by the lack of shared objects. To understand how shared objects are formed in U–S collaboration, this research established a university-school collaborated Change Laboratory in W primary school based on cultural-historical activity theory, which is under the background of Chinese teaching research activity.MethodsRecordings of meetings throughout the year were transcribed into texts and coded, and then analyzed via the method of grounded theory and contradiction analysis.ResultsThe findings reveal that, in comparison to previous studies regarding shared object formation process, this study identified an special phase named “experimental object,” which highlights the significance of experimentation in U–S collaboration. Also, multiple contradictions are recognized as the driving force for shared object formation which would gradually transform into fundamental conflicts between tools. The main contradictions identified include those between scientific and daily concepts, university culture and school culture, as well as new experiment and old routine.DiscussionThe current study implicates that U–S collaboration is an expansive learning process to acquire unknown knowledge, which necessitates both parties engaging in exploration and experimentation together. Furthermore, shared object formation within U–S collaboration requires participants to focus on developing teaching tools while consciously undergoing changes in aspects such as logic of thinking, culture and routine.
Potential categories of employment stress among rural college students and their relationship to employment psychology
Authors: Wu Xinyue , Kim Kyung Yee , Jian Ziting 
Published: March 2024

BackgroundPsychological problems related to employment are among the most common psychological problems faced by rural college students. Employment stress is an important factor affecting the development of psychological health in employees; thus, reducing employment stress can improve the psychological state of employment.ObjectiveThis study aimed to understand the potential profiles of employment stress among rural college students to determine the relationship between different profiles and employment psychology.MethodsThis study was conducted in a higher education institution in Qingdao, Shandong Province, China between June and December 2023, and 249 rural college students participated. The Employment Stress Scale and Employment Psychology Scale were used to collect the data. Data were analyzed using latent profile analysis, independent sample t-tests, and binary logistic regression analysis.ResultsThe results showed that rural university students were categorized into low-level (49.80%) and high-level (50.21%) employment stress groups. There was a statistically significant difference between the employment psychology of rural college students in the low- and high-level groups (p &lt; 0.001). Juniors/seniors were more likely to be classified in the high-level group (OR = 0.477, p = 0.011).ConclusionIntervention programs should be developed and implemented to address the characteristics of employment stress among rural college students with different profiles to promote the healthy development of their attitudes toward employment.
Korean autistic persons facing systemic stigmatization from middle education schools: daily survival on the edge as a puppet
Authors: Yoon Wn-ho , Seo JaeKyung , Je Cheolung 
Published: March 2024

IntroductionKorean autistic persons who have endured an integrated secondary education system have been exposed to school bullying, causing trauma and stigma to them. It also blocks them from entering a tertiary education system and a decent work, resulting in a lower quality of life. However, research on how it affects autistic persons has not yet been conducted in Korea.MethodsFourteen adult autistic persons in the Republic of Korea participated in the semi-structured focused group interviews. Their conversations were analyzed through qualitative coding.ResultsThe interview results show the rare voice of Korean autistic people. Although interviewees experienced physical, verbal, and sexual violence against them during the secondary education period, they could not get substantial assistance from schools and society. Interviewees agreed that bullying is inherent in the secondary education system of Korea, even in Korean culture. They experienced the cause of bullying being attributed to them as victims rather than perpetrators, and impunity is given to the bullying assailants. Early analyses of this article confirm that such experiences are combined with the sociocultural climate of elitism, meritocracy, and authoritarianism in the Republic of Korea.ConclusionThe study confirmed that the autistic person’s bullying experience does not come from the social inability of autistic people but the “profound” competition and discriminative atmosphere of the society. The result urges further studies on the bullying experience of East Asian autistic persons and the construction of Korean intervention strategies to prevent school violence against Koreans with disabilities, especially autistic pupils.
Emotion dysregulation links pathological eating styles and psychopathological traits in bariatric surgery candidates
Authors: Belloli Arianna , Saccaro Luigi F. , Landi Paola , Spera Milena , Zappa Marco Antonio , Dell’Osso Bernardo , Rutigliano Grazia 
Published: March 2024

ObjectivesApproximately one-third of bariatric surgery patients experience weight regain or suboptimal weight loss within five years post-surgery. Pathological eating styles and psychopathological traits (e.g., emotion dysregulation) are recognized as potential hindrances to sustain weight loss efforts and are implicated in obesity development. A comprehensive understanding of these variables and their interplays is still lacking, despite their potential significance in developing more effective clinical interventions for bariatric patients. We investigate the prevalence of and interactions between pathological eating styles and psychopathological traits in this population.Materials and methods110 bariatric surgery candidates were characterized using the Binge Eating Scale (BES), Hamilton Depression/Anxiety Scales (HAM-D/A), Barratt Impulsiveness Scale (BIS-11), Experiences in Close Relationships (ECR), Difficulties in Emotion Regulation Scale (DERS). We analyzed these variables with multiple logistic regression analyses and network analysis.ResultsPatients with pathological eating styles showed more pronounced anxiety/depressive symptoms and emotion dysregulation. Network analysis revealed strong connections between BES and DERS, with DERS also displaying robust connections with HAM-A/D and ECR scales. DERS and attention impulsivity (BIS-11-A) emerged as the strongest nodes in the network.DiscussionOur findings demonstrate the mediating role of emotion dysregulation between pathological eating styles and psychopathological traits, supporting existing literature on the association between psychopathological traits, insecure attachment styles, and pathological eating behaviors. This research emphasizes the significance of emotion regulation in the complex network of variables contributing to obesity, and its potential impact on bariatric surgery outcomes. Interventions focusing on emotion regulation may thus lead to improved clinical outcomes for bariatric patients.
Incidence of grass and weed sensitization in Bangkok, Thailand: a clinical study
Authors: Aud-in Sirirat , Juprasong Yotin , Pinkaew Bannapuch , Talek Kanokporn , Tantilipikorn Pongsakorn , Songnuan Wisuwat 
Published: March 2024

BackgroundAllergic rhinitis (AR) is a prevalent public health concern globally, significantly impacting quality of life. In Thailand, the prevalence of AR is rising, with grass and weed pollen identified as primary outdoor triggers.ObjectivesThis study aimed to (1) assess patterns of pollen sensitization in Thai AR patients and (2) investigate correlations between demographics/clinical data and SPT results.MethodsA total of 121 individuals aged ≥18 years with clinically diagnosed AR were recruited. Skin prick testing (SPT) was performed using a panel of commonly encountered tropical grass and weed pollen extracts. SPT wheal sizes and clinical symptom scores were recorded. Correlations between SPT outcomes and symptom scores were analyzed.ResultsAmong the participants, 104 (85.95%) exhibited positive SPT reactions to at least one pollen type. Nutsedge (76/121), para grass (57/121), and Bermuda grass (48/121) were the most frequently identified allergens. Hurricane grass elicited the strongest reaction, evidenced by the highest average wheal size (6.2 mm). Poly-sensitization was observed in 77 (63.6%) of the SPT-positive individuals, with most cases involving two different pollen extracts (35/77). Notably, AR severity positively correlated with both average wheal size and the number of positive SPT tests.ConclusionThis study highlights nutsedge, para grass, and Bermuda grass as major allergenic pollen sources for Thai AR patients. Including nutsedge, hurricane grass, and careless weed in clinical SPT panels is recommended for improved diagnostic accuracy. Additionally, the positive correlation between AR severity and pollen reaction strength emphasizes the importance of implementing patient education and avoidance strategies.
Work status among middle-aged and older individuals in China: the effects on physical and mental health
Authors: Fu Yi , Li Xiaohan 
Published: March 2024

ObjectiveChina’s middle-aged and older population is a rich source of human capital. Therefore, considering the health of this group is important when creating and using human resources.MethodsUsing data from the nationwide China Health and Retirement Longitudinal Study (CHARLS) 2018 baseline survey of 19,000 adults ages 45 years and older, this study was an objective investigation of the effects of work on the physical and mental health of middle-aged and older adults. We conducted several types of analyses using details of participants’ lifestyles and demographic characteristics (living environment, education, financial level, and access to medical services) with work (i.e., employment or volunteer work) as a primary input component of health production to examine their effects on the health status of middle-aged and older individuals.ResultsSix primary outcomes were identified: (1) Employment positively affects both the physical and mental health of middle-aged and older people; (2) Employment can influence the physical and mental health of middle-aged and older people through income, cognitive level, and social support networks; (3) Compared to non-self-employment, self-employment dramatically worsens physical health but has no apparent detrimental effects on mental health. Compared to non-agricultural employment, agricultural labor affects both physical and mental health. (4) Employment has more positive physical and mental health effects in Individuals with higher rather than lower levels of education. (5) Employment opportunities in the eastern region are more likely to promote better physical health than those in the central and western regions of China, although the effects on mental health are negative. (6) When considering groups at different ages of the lifespan, the 60–65-year-old group, who are just entering retirement probably benefit more from continuing work.ConclusionWe provide some recommendations to encourage middle-aged and older people to work and utilize their experience, which will enhance their physical and mental well-being and help them in realize their own value and social integration.