bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual Enzyme Sequential Digestion Protocol for Isolation of Human Primary Chondrocytes From the Articular Cartilage Derived From Knee Osteoarthritis Patients
Authors: Anuja Anamika Kumari , Rai Mohit Kumar , Gupta Latika , Nigam Neha , Agarwal Vikas 
Published: March 2024

Introduction: Majority of available protocols for isolation of chondrocytes from articular cartilage tissue rely on the enzymatic digestion of the tissue by collagenase type 2. The yield of chondrocytes in such protocols is low. Herein, we designed a novel indigenous sequential digestion by dual enzyme Pronase and Collagenase Type 1 for isolating human Chondrocytes from articular cartilage. Methods: Articular cartilage of Osteoarthritis (OA) patients undergoing total knee replacement were collected for the isolation of chondrocyte cells and subjected to sequential digestion by Pronase for three hours followed by Collagenase 1 overnight. Pellet of cells collected after digestion was plated on culture flask in 5% CO2 incubator. Results: From day three onwards, round to elongated cells adhered to the flask were visible which developed into elongated cell population of homogenous morphology, expressed Aggrecan (Agg), Collagen 2a (Col2a) and SRY-box transcription factor (Sox9) and had chondrogenic differentiation similar to a commercially available healthy chondrocyte. These cells were negative for Alizarin red stain, thus confirming the purity of chondrocytes. Conclusion: We have successfully established a sequential dual enzyme digestion-based culture technique for isolating the human chondrocytes from the articular cartilage biopsy derived from OA knee joints.
CCL21 and CLDN11 Are Key Driving Factors of Lymph Node Metastasis in Gastric Cancer
Authors: Yang Shaofei , Dong Dandan , Bao Xunxia , Lu Rongting , Cheng Pufei , Zhu Sibo , Yang Guanghua 
Published: March 2024

Background Gastric cancer (GC) is a leading cause of cancer-related deaths worldwide. Understanding the molecular mechanisms of GC metastasis is crucial for improving patient survival outcomes. Methods RNA sequencing and analysis were performed on tissue samples from primary and lymph node metastatic lesions of gastric cancer. Differential gene analysis and functional pathway analysis were conducted. Immune infiltrating environment and protein expression levels were evaluated using immunohistochemistry. Cell experiments were conducted to investigate the role of CCL21 in GC metastasis. Results ACTG2, CNN1, DES, MUC6, and PGC were significantly upregulated in primary tumor cells, while CCL21, MS4A1, CR2, CLDN11, and FDCSP were significantly upregulated in metastatic tumor cells. Functional pathway analysis revealed enrichment in pathways related to immune response. CLDN11 and CCL21 were found to play important roles in promoting gastric cancer metastasis. Cell experiments confirmed the role of CCL21 in promoting GC cell growth and metastasis. CCL21 is highly expressed in GC tissues and binds to CCR7, leading to upregulation of CLDN11. This results in GC-lymph node metastasis and abnormal activation of immune cells (B cells and CD4+ T cells). Conclusion Inhibition of CCL21 and CLDN11 proteins may be a promising strategy for treating GC and preventing lymph node metastasis. These findings provide specific molecular markers for early lymph node metastases of GC, which can aid in developing treatment strategies and predicting patient prognosis.
Prevalence and Associated Factors of Work-Related Injury Among Municipal Solid Waste Collectors in Hawassa City, Southern Ethiopia: A Cross-Sectional Study
Authors: Ejeso Amanuel , Berego Yohannes Seifu , Ahmednur Mahmud 
Published: March 2024

Introduction: Municipal solid waste collectors are at risk of experiencing work-related health problems due to the waste they handle and the physical effort they put in. Work-related injuries among domestic waste collectors have been the subject of scant research in developing nations. Therefore, it is necessary to describe work-related injuries in these subpopulation groups. Objective: This study aimed to determine the prevalence and associated factors of work-related injury among municipal solid waste collectors in Hawassa city, Southern Ethiopia. Methodology: A cross-sectional study was conducted from January 11 to 30, 2022, with all the registered municipal solid waste collectors (411). Three diploma environmental health professionals assisted in collecting data using a pretested structured interviewer-administered questionnaire; 1 Bachelor of Science environmental health professional served as a supervisor. Statistical Package for the Social Sciences (SPSS) version 20.0 was used to enter, clean, code, and analyze the data. Associations between independent and dependent variables were assessed, and the strength of the associations was presented by using Adjusted Odds Ratio (AOR) and 95% confidence intervals (CI). According to the bivariable analysis, all independent variables associated with a P-value <.25 were entered into a multivariable logistic regression model. Variables in the multivariable regression analysis were considered significant if their P-value was less than 0.05. Results: In this study, the prevalence of work-related injuries within the last 30 days was 12.9% (95% CI: 9.5, 16.3), while the prevalence over the last 12 months was 59.4% (95% CI: 54.7, 64.0). After adjusting for possible confounding factors, age 18 to 24 years [AOR = 1.72;95% CI: (1.07, 2.76)], lack of personal protective equipment (PPE) utilization [AOR = 2.30; 95% CI: (1.44, 3.68)], chewing khat (yes) [AOR = 2.32;95% CI: (1.32, 4.08)], drinking alcohol (yes) [AOR = 1.80; 95% CI: (1.003, 3.24)] and job dissatisfaction [AOR = 2.26, 95% CI: (1.44, 3.54)] were shown to be significantly associated with work-related injury. Conclusion: The prevalence of work-related injuries was high. Age 18 to 24 years, chewing khat, drinking alcohol, lack of PPE utilization, and job dissatisfaction were significantly associated with work-related injury. Based on the findings of this study, we suggest that addressing these risk factors could help to reduce work-related injuries.
Reducing no-show visits and disparities in access: The impact of telemedicine
Authors: Shao Connie C , Katta Meghna H , Smith Burke P , Jones Bayley A , Gleason Lauren T , Abbas Alizeh , Wadhwani Nikita , Wallace Eric L , Mugavero Michael J , Chu Daniel I 
Published: April 2024

Background No-show visits have serious consequences for patients, providers, and healthcare systems as they lead to delays in care, increased costs, and reduced access to services. Telemedicine has emerged as a promising alternative to in-person visits by reducing travel barriers, but risks exacerbating the digital divide. The aim of this study was to assess the impact of telemedicine (video and phone) at a tertiary care academic center on no-show visits compared to in-person visits. Methods A retrospective cohort analysis of all weekday clinic visits among in-state adult patients at a single tertiary care center in the southeast from January 2020 to April 2023 was performed. Rates of no-show visits for patients who were seen via phone and video were compared with those who were seen in-person. Demographic and clinical characteristics of these groups were also compared, including age, sex, race/ethnicity, socioeconomic status, and visit type. The primary outcome was the rate of no-show visits for each visit type. Results Our analysis included 3,105,382 scheduled appointments, of which 81.2% were in-person, 13.4% via video, and 5.4% via phone calls. Compared to in-person visits, phone calls and video visits reduced the odds of no-show visits by 50% (aOR 0.5, CI 0.49–0.51) and 15% (aOR 0.85, CI 0.84–0.86), respectively. Older patients, Black patients, patients furthest from clinic, and patients from counties with the greatest degree of vulnerability and disparities in digital access were more likely to use phone visits. No-shows were more common among non-white, male, and younger patients from counties with lower socioeconomic status. Conclusion Telemedicine effectively reduced no-show visits. However, limiting telemedicine to video-based visits only exacerbated disparities in access. Phone calls allow historically underserved patients from lower socioeconomic backgrounds to access healthcare and should be included within the definition of telemedicine.
Abnormal Colorectal Cancer Test Follow-Up: A Quality Improvement Initiative at a Federally Qualified Health Center
Authors: Shareef Faizah , Bharti Balambal , Garcia-Bigley Felipe , Hernandez Monica , Nodora Jesse , Liu Jie , Ramers Christian , Nery Jill Dumbauld , Marquez Jessica , Moyano Karina , Rojas Sarah , Arredondo Elva , Gupta Samir 
Published: March 2024

Introduction/Objectives: Colonoscopy completion rates after an abnormal fecal immunochemical test (FIT) are suboptimal, resulting in missed opportunities for early detection and prevention of colorectal cancer. Patient navigation and structured follow-up may improve colonoscopy completion, but implementation of these strategies is not widespread. Methods: We conducted a quality improvement study using a Plan-Do-Study-Act (PDSA) Model to increase colonoscopy completion after abnormal FIT in a large federally qualified health center serving a diverse and low-income population. Intervention components included patient navigation, and a checklist to promote completion of key steps required for abnormal FIT follow-up. Primary outcome was proportion of patients achieving colonoscopy completion within 6 months of abnormal FIT, assessed at baseline for 156 patients pre-intervention, and compared to 208 patients during the intervention period from April 2017 to December 2019. Drop offs at each step in the follow-up process were assessed. Results: Colonoscopy completion improved from 21% among 156 patients with abnormal FIT pre-intervention, to 38% among 208 patients with abnormal FIT during the intervention ( P < .001; absolute increase: 17%, 95% CI: 6.9%-25.2%). Among the 130 non-completers during the intervention period, lack of completion was attributable to absence of colonoscopy referral for 7.7%; inability to schedule a pre-colonoscopy specialist visit for 71.5%; failure to complete a pre-colonoscopy visit for 2.3%; the absence of colonoscopy scheduling for 9.2%; failure to show for a scheduled colonoscopy for 9.2%. Conclusions: Patient navigation and structured follow-up appear to improve colonoscopy completion after abnormal FIT. Additional strategies are needed to achieve optimal rates of completion.
Axicabtagene ciloleucel vs standard of care in second-line large B-cell lymphoma: outcomes by metabolic tumor volume
Authors: Locke Frederick L. , Oluwole Olalekan O. , Kuruvilla John , Thieblemont Catherine , Morschhauser Franck , Salles Gilles , Rowe Steven P. , Vardhanabhuti Saran , Winters Joshua , Filosto Simone , To Christina , Cheng Paul , Schupp Marco , Korn Ronald , Kersten Marie José 
Published: April 2024

Abstract
Metabolic tumor volume (MTV) assessed using 2-deoxy-2-[18F]fluoro-d-glucose positron emission tomography, a measure of tumor burden, is a promising prognostic indicator in large B-cell lymphoma (LBCL). This exploratory analysis evaluated relationships between baseline MTV (categorized as low [median or less] vs high [greater than median]) and clinical outcomes in the phase 3 ZUMA-7 study (NCT03391466). Patients with LBCL relapsed within 12 months of or refractory to first-line chemoimmunotherapy were randomized 1:1 to axicabtagene ciloleucel (axi-cel; autologous anti-CD19 chimeric antigen receptor T-cell therapy) or standard care (2-3 cycles of chemoimmunotherapy followed by high-dose chemotherapy with autologous stem cell transplantation in patients who had a response). All P values are descriptive. Within high- and low-MTV subgroups, event-free survival (EFS) and progression-free survival (PFS) were superior with axi-cel vs standard care. EFS in patients with high MTV (vs low MTV) was numerically shorter with axi-cel and was significantly shorter with standard care. PFS was shorter in patients with high MTV vs low MTV in both the axi-cel and standard-care arms, and median MTV was lower in patients in ongoing response at data cutoff vs others. Median MTV was higher in patients treated with axi-cel who experienced grade ≥3 neurologic events or cytokine release syndrome (CRS) than in patients with grade 1/2 or no neurologic events or CRS, respectively. Baseline MTV less than or equal to median was associated with better clinical outcomes in patients receiving axi-cel or standard care for second-line LBCL. The trial was registered at www.clinicaltrials.gov as #NCT03391466.
B-cell–directed CAR T-cell therapy activates CD8+ cytotoxic CARneg bystander T cells in patients and nonhuman primates
Authors: Kaminski James , Fleming Ryan A. , Alvarez-Calderon Francesca , Winschel Marlana B. , McGuckin Connor , Ho Emily E. , Eng Fay , Rui Xianliang , Keskula Paula , Cagnin Lorenzo , Charles Joanne , Zavistaski Jillian , Margossian Steven P. , Kapadia Malika A. , Rottman James B. , Lane Jennifer , Baumeister Susanne H. C. , Tkachev Victor , Shalek Alex K. , Kean Leslie S. , Gerdemann Ulrike 
Published: April 2024

Abstract
Chimeric antigen receptor (CAR) T cells hold promise as a therapy for B-cell–derived malignancies, and despite their impressive initial response rates, a significant proportion of patients ultimately experience relapse. Although recent studies have explored the mechanisms of in vivo CAR T-cell function, little is understood about the activation of surrounding CARneg bystander T cells and their potential to enhance tumor responses. We performed single-cell RNA sequencing on nonhuman primate (NHP) and patient-derived T cells to identify the phenotypic and transcriptomic hallmarks of bystander activation of CARneg T cells following B-cell–targeted CAR T-cell therapy. Using a highly translatable CD20 CAR NHP model, we observed a distinct population of activated CD8+ CARneg T cells emerging during CAR T-cell expansion. These bystander CD8+ CARneg T cells exhibited a unique transcriptional signature with upregulation of natural killer-cell markers (KIR3DL2, CD160, and KLRD1), chemokines, and chemokine receptors (CCL5, XCL1, and CCR9), and downregulation of naïve T-cell-associated genes (SELL and CD28). A transcriptionally similar population was identified in patients after a tisagenlecleucel infusion. Mechanistic studies revealed that interleukin-2 (IL-2) and IL-15 exposure induced bystander-like CD8+ T cells in a dose-dependent manner. In vitro activated and patient-derived T cells with a bystander phenotype efficiently killed leukemic cells through a T-cell receptor–independent mechanism. Collectively, to our knowledge, these data provide the first comprehensive identification and profiling of CARneg bystander CD8+ T cells following B-cell–targeting CAR T-cell therapy and suggest a novel mechanism through which CAR T-cell infusion might trigger enhanced antileukemic responses. Patient samples were obtained from the trial #NCT03369353, registered at www.ClinicalTrials.gov.
The Mediator Subunit OsMED16 Interacts with the WRKY Transcription Factor OsWRKY45 to Enhance Rice Resistance Against Magnaporthe oryzae
Authors: Wu Yanfei , Fu Yuquan , Zhu Zhonglin , Hu Qin , Sheng Feng , Du Xuezhu 
Published: April 2024

AbstractRice blast, caused by Magnaporthe oryzae (M. oryzae), is one of the most common and damaging diseases of rice that limits rice yield and quality. The mediator complex plays a vital role in promoting transcription by bridging specific transcription factors and RNA polymerase II. Here, we show that the rice mediator subunit OsMED16 is essential for full induction of the diterpenoid phytoalexin biosynthesis genes and resistance to the ascomycetous fungus M. oryzae. Mutants of Osmed16 show reduced expression of the DP biosynthesis genes and are markedly more susceptible to M. oryzae, while transgenic plants overexpressing OsMED16 increased the expression of the DP biosynthesis genes and significantly enhanced resistance to M. oryzae. Interestingly, OsMED16 is physically associated with the WRKY family transcription factor OsWRKY45, which interacts with the phytoalexin synthesis key regulator transcription factor OsWRKY62. Further, OsMED16-OsWRKY45-OsWRKY62 complex could bind to the promoter regions of phytoalexin synthesis-related genes and activate their gene expression. Our results show that OsMED16 may enhance rice tolerance to M. oryzae via directly manipulating phytoalexin de novo biosynthesis.
Unveiling the potential of a functionalized pyrrole-based polymer for efficient cadmium ion removal from wastewater: synthesis, characterization, and performance evaluation
Authors: Sayed Marwa M. , El-Hamid Islam S. Abd , El-Bery Haitham M. , Farrag Mostafa , Aly Kamal I. 
Published: April 2024

AbstractCadmium ion Cd2+ contamination is a major environmental issue caused by industry. Polyarylidene N-hexane pyrrole (PAPh) and crosslinked polyarylidene N-phenyl pyrrole (PAPD) were prepared from the previously synthesized polymer (polyarylidene ketone (PAK)) by using the advantage of repeating carbonyl groups at the 1,4 position and reacting it with hexylamine and P-phenylenediamine via the Paal–Knorr reaction. Various methods were used to characterize polymers, such as FT-IR spectroscopy, X-ray diffraction (XRD), thermogravimetry analysis (TGA), UV–visible spectroscopy, scanning electron microscope (SEM), zeta potential, and surface area measurements (BET), revealing successful fabrication, good thermostability, and well-defined microporous structures useful for Cd2+ adsorption. Optimal adsorption capacities of 55.8 mg g−1 for PAPh and 86.95 mg g−1 for PAPD indicate a significant enhancement in Cd2+ adsorption via their microporous structures, Cd2+ adsorption was also investigated in terms of contact time, initial concentration, and pH. A total input concentration of 30 ppm Cd ions, may yield an 84.3% removal rate for PAPh and an 89.2% removal rate for PAPD. The experimental results were well-fit by many models, including pseudo-second-order kinetics (PSO), Freundlich isotherms, intraparticle diffusion, and Langmuir. The varying adsorption performances of the two polymers studied, (PAPh) and (PAPD), were found to be derived from their respective chemical structures, which include various functional groups, according to studies conducted on Cd2+ in an aqueous solution. Cd2+ adsorption on polymers was considered physisorption; π–cation interactions and surface complexation played significant roles in adsorption. The PAP polymers may be considered promising substitutes and innovative adsorbents to remove Cd2+ ions from a water solution.
Data collection for assessment of the natural capital at the regional level: case study of LTSER Trnava region
Authors: Izakovičová Zita , Miklos Laszlo , Spulerova Jana , Dobrovodská Marta , Halada Ľuboš , Raniak Andrej , Dick Jan 
Published: April 2024

Abstract
Context
The landscape provides not only a living space for all life forms, including humans, but also a spatial base and set of resources for the implementation of individual human activities. Inappropriate implementation of human activities, disrespecting the properties of the landscape's natural resources, causes the degradation of natural resources and, consequently, the human living.

Objectives
The aim of this paper is to develop new methodological procedures and algorithms for effective assessment of natural capital based on the geosystem approach.

Methods
Each territorial unit (geosystem) represents a unique combination of natural assets that create a certain potential for the development of individual activities and eco-stabilization functions. In this study, we developed a new approach and algorithms to assess the natural capital of landscapes for sustainable use. This involves selecting indicators and their functional interpretation, as well as collecting available spatial data and statistics for GIS analysis, synthesis, and modeling.

Results
The methodological procedure consists of the determination of indicators for natural capital assessment, the determination of their functional values and weighting coefficients, the determination of the suitability of the geosystem for the implementation of individual activities based on the value of natural capital, and the determination of restrictions and limiting factors. The set of data on landscape assets can be categorized into abiotic, land cover and biotic, and socio-economic indicators, which can either support human activities or limit them. Options for sustainable use of natural capital were split into two groups of potential activities: (I) natural capital for landscape planning activities and (II) specific activities or functions (e.g., natural capital for energy use, recreation, regulation services). The modeling of eco-stabilizing natural capital in Trnava LTSER pointed to low spatial ecological stability, mainly in the central part of the district.

Discussion
Discussion pointed to strength, novelty and opportunities of implementing methodological approach to natural capital assessment.

Conclusions
As an output of this methodological approach, a comprehensive digital spatial database of landscape-ecological data for the assessment of natural capital and the suitability of its use for socio-economic activities has been created in Slovakia. The database represents a set of consistent spatial information on natural capital assets and other indicators, including land cover and socio-ecological indicators. The methodological approach can be applicable to any territory on the basis of a modification.