bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative transcriptome analysis of differentially expressed genes in the ovary and testis and identification of transformer-2 gene of Athetis dissimilis
Authors: Song Yueqin , Han Shengjie , Zhang Tiansi , Hao Haohao , Sun Hui-Zhong 
Published: March 2024

Abstract

Background
Insects have developed a variety of sex-determining regulatory mechanisms throughout their evolution. Not only do different insects use different genes and regulatory mechanisms for sex differentiation, but different subspecies populations of the same insect species can have different sex determination mechanisms. We analyzed differentially expressed genes (DEGs) from the ovary and testis transcriptomes of A. dissimilis. The function of DEGs was analyzed from various databases. The results of this research will aid in the understanding of the sex-determination mechanisms of this species.
Results
A total of 11,065 DEGs between the ovary and testis were identified, of which 6,685 genes were up-regulated and 4,380 genes were down-regulated in testis. 6,656 DEGs were annotated in NR (6,616, 99.40%), GO (1,484, 22.30%), COG (1,696, 25.48%), KEGG (2,483, 37.30%), Swiss-Prot (3,077, 46.23%), Pfam (3,967, 59.60%), and eggNOG (5,808, 87.26%). A Adistra-2 (525 bp) gene was obtained from the transcriptome of A. dissimilis, and sequence alignment with other related species revealed a highly conserved RRM domain. Phylogenetic analysis showed that the Adistra-2 protein is a close relative of the lepidopterous tra-2 protein. The temporal and spatial expression pattern of the Adistra-2 showed that it is more abundant during embryonic development than other stages, and its expression was higher in ovaries than in testes.
Conclusions
We analyzed DEGs from the ovary and testis transcriptomes of Athetis dissimilis. The characteristics of Adistra-2 gene were identified and analyzed. This work provides a theoretical reference for the sex differentiation and genetic manipulation of this insect.
Quantum interference comparison between benzene, naphthalene, and azulene single molecular junction
Authors: Baker Naji Abdulrazzaq , Al-Jobory Alaa A. 
Published: March 2024

Abstract
The ability to build devices from single-molecular junctions depends on the fabrication of molecular structures. Here we report significant changes in the transmission coefficient for different connectivities for polycyclic aromatichydrocarbons (PAHs). In this study, we are using density functional theory DFT compared with the single orbital tight binding TB to investigate the transmission coefficient for three different molecules which are benzene, naphthalene and azulene with different point connections with electrodes. In the case of benzene the para connection, are leads to constructive quantum interference CQI, and the meta link is distractive quantum interference DQI. Next naphthalene and azulene have the same number of atoms, with five different connections, the result shows the even-even or odd-odd connection are DQI while the even-odd is CQI. This result is supported by the wave function molecular orbital plot. Finally, the TB calculations are in good agreement with DFT.
Decreased expression of the HDAC2 disrupts the SLIT-ROBO signaling pathway and induced angiogenesis in placental endothelial cells in preeclampsia
Authors: Fan Xufei , Zheng Xiujuan , Malik Samiullah , Yu Jianyun , Yang Yali 
Published: March 2024

Abstract

Background: Preeclampsia is characterized by reduced histone deacetylase 2 (HDAC2) expression in placental tissue HDAC2 enrichment positively affects angiogenesis as it helps prevent endothelial cell dysfunction. Additionally, research has demonstrated that the SLIT2-ROBO signaling pathway influences preeclampsia. Bioinformatics analysis has suggested that HDAC2 may have a transcriptional regulatory effect on SLIT2. Consequently, investigations have examined the relationship between low HDAC2 expression and the SLIT-ROBO signaling pathway in placental angiogenesis in patients with preeclampsia.
Objective: To investigate how decreased HDAC2 expression disrupts the SLIT-ROBO signaling pathway and induces angiogenesis in placental endothelial cells in preeclampsia.
Methods:The study included patients with preeclampsia as the observation group, while the placental tissue of normal pregnant women was used as the in vivo control model. In vitro endothelial models using human umbilical veins and microvascular endothelial cells were also used to examine the effects of interference with the expression of HDAC2 and SLIT2. Cell viability CCK-8, colony formation, and tube formation assays were conducted to evaluate angiogenesis. Furthermore, Immunohistochemistry, RT-qPCR, and Western blot analyses were used to examine the expression of genes in cells and tissues.
Results: The expression of SLIT2 and ROBO1 was increased, and the protein and mRNA expression of CD34, HDAC2, and vonWillebrand factor(vWF) were lower in preeclampsia placentas than in normal placentas. Using an in vitro endothelial model, the knockdown of HDAC2 inhibited colony formation and impaired neovascularization by reducing vascular endothelial growth factor A (VEGFA) and vascular endothelial growth factor -2 (VEGFR2) activity, while SLIT2 and ROBO1 were highly expressed. The changes caused by HDAC2 knockdown were reversed by SLIT2 knockdown.
Conclusion: Preeclampsia progression is promoted by low HDAC2 expression, which inhibits the SLIT-ROBO signaling pathway and induces angiogenesis in placental endothelial cells.
Accurate Analysis of The Distribution of Medical Imaging Technologies: The Case of Türkiye
Authors: Temiz Hakan , Kara Tuncay 
Published: March 2024

Abstract
The distribution of medical diagnostic imaging technologies (MDIT) between the regions of Türkiye were analyzed through hierarchical clustering of the capacity and use (CAU) variables. Statistics yearbooks’ data of the Ministry of Health for the years 2013 and 2018 were used. Analyses were performed on the entire CAU data and its six subsets generated according to the characteristics of MDIT. The dissimilarities of the clusters were measured by Euclidean distance between z-score standardized data points. The experiments showed that analyzing the CAU variables together may lead to the formation of the clusters that do not reflect reality and therefore to make erroneous judgments. To ensure more accurate and detailed analysis, they should be analyzed on a per-device basis. This study also showed that 12 Nomenclature of Territorial Units for Statistics (NUTS) regions of the country could be clustered within 3 to 5 groups. According to our analysis method, with some negligible exceptions, it is seen that Turkey is in a very good position in ensuring the equity in access to the health services, in terms of MDIT distribution.
Examining Emotional and Behavioural Trajectories in Siblings of Children with Life-Threatening Conditions
Authors: Tay Joanne , Widger Kimberley , Steele Rose , Stremler Robyn , Pole Jason D. 
Published: March 2024

Abstract

Background
Healthy siblings of children with life-limiting conditions often experience emotional and behavioral struggles over the course of the ill child’s condition(s). Resources to support these siblings are limited due to the lack of understanding about the needs of siblings. Therefore, this study was designed to characterize the emotional and behavioral trajectories of siblings of children with progressive, life-limiting genetic, metabolic, or neurological conditions over a 12-month observation period.
Methods
Seventy siblings were recruited from a large survey-based study (Charting the Territory) that examined the biopsychosocial health outcomes of parents and siblings. Linear mixed effect models were used to assess the associations between siblings’ emotions and behavior trajectories and selected demographic variables. Siblings’ emotions and behaviors were measured with Child Behavior Checklist (CBCL).
Results
The mean age of the siblings was 11.2 years at baseline, and the Internalizing, Externalizing, and Total Behavior Problems mean scores were within normal ranges across time. However, 7–25% of the siblings had scores within the clinical range. Brothers had higher levels of internalizing problems than sisters, whereas sisters had higher levels of externalizing problems than brothers. When treatment was first sought for the ill child less than a year prior to study participation, the siblings had higher levels of internalizing and externalizing problems than did the siblings who participated more than one year after treatment was sought.
Conclusion
Healthy siblings experience emotional and behavioral problems early in the child’s disease trajectory. Although these problems improve with them, our findings show that brothers and sisters experience different types of challenges. Therefore, timely support for siblings is important as they navigate through these uncertainties and challenges.
Perceptual Autoencoder and Exemplar Selection for Lifelong Learning in Convolutional Neural Networks (CNNs)
Authors: Nugroho Hermawan , Tay Gee Yang , Dube Swaraj 
Published: March 2024

Abstract
Lifelong learning or incremental learning in convolutional neural networks (CNNs) has encountered a challenge known as catastrophic forgetting, which impairs model performance when tasks are presented sequentially. While a simple approach of retraining the model with all previously seen training data can alleviate this issue to some extent, it is not scalable due to the rapid accumulation of storage requirements and retraining time. To address this challenge, we propose a novel incremental learning strategy involving image data generation and exemplar selection. Specifically, we introduce a new type of autoencoder called the Perceptual Autoencoder, which reconstructs previously seen data while significantly compressing it, requiring no retraining when new classes are introduced. The latent feature map from the undercomplete Perceptual Autoencoder is stored and utilized to reconstruct training data for replay alongside new class data when necessary. Additionally, we employ example forgetting as an exemplar detection metric for exemplar selection, aiming to minimize the number of old task training data while preserving model performance. Our proposed strategy achieves state-of-the-art performance on both CIFAR-100 and ImageNet-100 datasets.
Towards new bioactive fluorine-containing 1,3,4-oxadiazole-amide derivatives: Synthesis, antibacterial activity, molecular docking and molecular dynamics simulation study
Authors: Xiong Fei , Zhang Yanjun , Jiao Jinlong , Zhu Yiren , Mo Tianlu , Li Yeji 
Published: March 2024

Abstract

Through the approach of molecular hybridization, this study rationally designed and synthesized new trifluoromethyl-1,3,4-oxadiazole amide derivatives, denoted as 1a-1n. The findings reveal that these novel molecules exhibit potent inhibitory effects against various bacterial strains. Thereinto, compounds 1c, 1d, 1i, 1j and 1n, demonstrate relatively superior antimicrobial performance against B. cereus FM314, with a minimum inhibitory concentration (MIC) of 0.03907 µg/mL. Molecular docking analysis suggests the potential importance of the Ser57 and Thr125 amino acid residues in contributing to the inhibitory activity against B. cereus. The consistency of these results was further corroborated through subsequent molecular dynamics simulations and MMPBSA validations. The insights gained from this study serve to facilitate the rational design and efficient development of novel eco-friendly antimicrobial inhibitors based on the trifluoromethyl-1,3,4-oxadiazole amide scaffold.
Determination of COVID-19 intensive care costs in Germany and assessment of the economic impact on the healthcare system - a nationwide retrospective cohort study from January 2020 to March 2022
Authors: Lottes Matthäus , Kaier Klaus , Graves Nicholas , Wolkewitz Martin 
Published: March 2024

Abstract
Background
In late 2021, the German statutory health insurance (SHI) funds reported average expenditures for the treatment of coronavirus disease 2019 (COVID-19) intensive care patients without mechanical ventilation at €8,000 and even more than €30,000 for those with mechanical ventilation. In this context, there is currently no further information on the total COVID-19 intensive care costs or an assessment of the economic impact on the German health care system.
Methods
A retrospective cohort study was conducted from January 01, 2020, to March 31, 2022, using nationwide inpatient billing data and the proportion of mechanically ventilated COVID-19 intensive care patients in Germany. The COVID-19 intensive care costs were then determined based on reported cost accounting estimates for nonventilated and ventilated intensive care unit (ICU) bed days. The health economic impact was assessed by relating the COVID-19 intensive care costs to SHI expenditures and the cost of illness for the German hospital sector.
Results
By the end of March 2022, German hospitals had spent a total of 2.2 million ICU bed days, including 1.2 million (55%) ventilated ICU bed days, for COVID-19 intensive care patients. Up to this point, the corresponding treatment costs were almost €3.0 billion. The annual COVID-19 intensive care costs more than doubled from €776 million in 2020 to €1.7 billion in 2021. This meant a cost increase of €203.1 million to €486 million for nonventilated ICU bed days and €573.2 million to €1.2 billion for ventilated ICU bed days. The cost share of COVID-19 intensive care in SHI expenditures for hospital treatment was predominantly less than 2.0%, and approximately 6.0% of the cost of illness for the hospital sector in 2020.
Conclusions
In conclusion, COVID-19 intensive care has become a rapidly growing cost factor for the German healthcare system, with the frequent use of mechanical ventilation being an important cost driver. However, compared to the SHI expenditures and cost of illness for the hospital sector, the cost share of COVID-19 intensive care appeared rather low. A more valid assessment of the health economic impact of COVID-19 intensive care in Germany requires case-specific billing data and consideration of costs associated with the displaced treatment of non-COVID-19 patients.
Research on WiFi human gesture recognition system based on hybrid model of DSC-SGRU
Authors: He Yulang , Zhao Zhibiao , Li Shanshan , Li Zhen 
Published: March 2024

Abstract
Wi-Fi wireless sensing technology has become a research hotspot in the field of perception, which can realize the intelligent perception of human activities and the surrounding environment. Aiming at the problem that the existing wireless sensing models have a large number of parameters, which makes it difficult to sense in real-time in scenarios with limited computing power, such as mobile devices, a lightweight feature extraction module based on Depthwise Separable Convolution (DSC) mixed with Stacked Gate Recur-rent Unit (SGRU) is proposed as a recognition model. The model first captures the spatial features of human gestures using DSC and keeps the temporal characteristics of the features unchanged, and then learns the spatio-temporal characteristics of the gestures using the SGRU network. The performance of the model is validated using the open source dataset Widar. The results show that the proposed DSC-SGRU model has only 236.891 K parameters with an accuracy of 77.6\%. Compared with existing gesture recognition models, DSC-SGRU greatly reduces the number of parameters of the model with approximate performance.
Evaluation of surgical strategies and long-term outcomes in pediatric hepatocellular carcinoma
Authors: Karayazili Merve , Celtik Ulgen , Ataseven Eda , Nart Deniz , Ergun Orkan 
Published: March 2024

Abstract
Purpose: Hepatocellular carcinoma (HCC), the second most common pediatric malignant liver tumor after hepatoblastoma, represents 1% of all pediatric tumors.
Methods A retrospective study was conducted on children with HCC treated at our center from March 2002-October 2022, excluding those with inadequate follow-up or records. Demographic data, initial complaints, AFP values, underlying disease, size and histopathological features of the masses, chemotherapy and long-term outcomes were analyzed.
Results Fifteen patients (8 boys, 7 girls) with a mean age of 11.4 ±4.1 years (0.8-16.4 years) were analyzed. The majority presented with abdominal pain, with a median AFP of 3.9 ng/mL. Hepatitis B cirrhosis in one patient (6.6%), metabolic disease (tyrosinemia type 1) in two patients (13.3%) were the underlying disease. Histopathological diagnoses were Fibrolamellar HCC (n:8; 53.3%), HCC (n:6; 40%). Four of the 15 patients underwent liver transplantation, 9 underwent surgical resection. Due to late diagnosis, 2 patients were considered inoperable (13.3%). The survival rate for the four patients who underwent liver transplantation was found to be 75%.
Conclusion: The surgical treatment of various variants of HCC, can be safely performed in experienced centers with a multidisciplinary approach and outcomes are better than in adults.
The Role of Parental Consanguinity and Familial Aggregation in Development of Multiple Sclerosis: A Case-control Study
Authors: Vaheb Saeed , Panah Mohammad Yazdan , Afshari-Safavi Alireza , Ghaffary Elham Moases , Shaygannejad Aysa , Shaygannejad Vahid , Mirmosayyeb Omid 
Published: March 2024

Abstract

Background
Several studies pointed out the importance of genetic risk factors such as parental consanguinity (PC) and familial multiple sclerosis (FMS) in the risk of MS. This study aimed to investigate the PC and FMS among people with MS (pwMS) in Isfahan, Iran.
Methods
This case-control study was conducted on pwMS from the MS clinic of Kashani Hospital, Isfahan, Iran, between September 2022 and September 2023. Healthy controls (HC) were also recruited from the MS clinic. Data on demographic and clinical characteristics and history of PC and FMS were collected from participants. The relationships between PC, FMS, and developing MS were assessed using multinomial logistic regression analysis. The Odds ratio (OR) with a 95% confidence interval (CI) was computed.
Results
A total number of 4264 pwMS and 400 HCs were included. The prevalence of PC and FMS among pwMS were 29.3% and 24%, respectively. Multinomial logistic regression adjusted for age and sex indicated that the odds of developing MS were significantly associated with a history of PC (OR = 3.03, 95%CI: 2.23 to 4.13, p < 0.001) and FMS (OR = 5.42, 95%CI: 3.51 to 8.38, p < 0.001).
Conclusion
PC and FMS can increase the risk of developing MS. PC and FMS should be considered along with other risk factors for developing MS. A comprehensive conclusion requires further research.
Associations of serum Cu, Zn, and Se with Chronic Respiratory Diseases in the American adults: Data from NHANES 2013–2016 and a bidirectional Mendelian randomization analysis
Authors: Yu Hanming , LV Taihong , Ji Zishuo , Wang Shuo , Liu Shi , Chen Yuru , Zhao Qi , Ma Li , Gu Xiu 
Published: March 2024

Abstract
Background
Despite studies on trace metals and Chronic Respiratory Diseases (CRDs), their causal relationship in American adults is uncertain. Our research applied observational and bidirectional Mendelian randomization (MR) methods to ascertain this association.
Objective
The aim of this study was to evaluate the potential linkages between serum levels of copper (Cu), zinc (Zn), and selenium (Se) and the incidence of chronic respiratory diseases within the adult demographic of the United States.
Methods
Drawing on the National Health and Nutrition Examination Survey (NHANES) 2013–2016 data, our study incorporated 2807 adults to examine serum Cu, Zn, and Se impacts on CRDs risks using logistic regression and cubic spline analysis. Subsequently, bidirectional MR assessments were conducted to ascertain the causative linkages pertaining to these associations.
Results
Employing adjusted-weighted logistic models, high serum Cu significantly escalated the risks for emphysema (OR: 3.83) and Chronic obstructive pulmonary disease (COPD) (OR: 2.14–3.33), while elevated Cu and Zn levels corresponded to respective OR of 1.82 and 1.91 for chronic tracheitis and COPD. Conversely, moderate Se reduced tracheitis risk (OR: 0.64). MR indicated no genetic causation between Cu and respiratory diseases (p > 0.05), yet Cu exposure was causally linked to COPD (p = 0.003), as was Zn (p = 0.014). selenium’s protective genetic association with chronic tracheitis was confirmed (p = 0.010).
Conclusion
High serum Cu links to increased emphysema, tracheitis, and COPD risk; Zn equally raises COPD risk; Se may lessen tracheitis risk. This observation warrants confirmation through additional large-scale, prospective cohort studies with sufficient sample sizes and extended follow-up durations.
Plasma Calcitonin Gene-Related Peptide Levels in Idiopathic Intracranial Hypertension: An Exploratory Study
Authors: Krajnc Nik , Frank Florian , Macher Stefan , Michl Martin , Müller Nina , Maier Sarah , Zaic Sina , Wöber Christian , Pemp Berthold , Broessner Gregor , Bsteh Gabriel 
Published: March 2024

Abstract
Background.
Idiopathic intracranial hypertension (IIH) is a debilitating condition characterized by increased intracranial pressure often presenting with chronic migraine-like headache. Calcitonin gene-related peptide (CGRP) plays an important pathophysiological role in primary headaches such as migraine and cluster headache, whilst its role in IIH has not yet been established.
Methods.
This longitudinal exploratory study included patients with definite IIH (pwIIH), episodic migraine (EM) in a headache-free interval and healthy controls (HC). Blood samples were collected from a cubital vein and plasma CGRP (pCGRP) levels were measured by standardized ELISA.
Results.
A total of 26 pwIIH (mean age 33.2 years [SD 9.2], 88.5% female, median BMI 34.8 kg/m2 [IQR 30.0–41.4]), 30 patients with EM (mean age 27.6 years [7.5], 66.7% female) and 57 HC (mean age 25.3 years [5.2], 56.1% female) were included. pCGRP levels displayed a wide variation in IIH as well as in EM and HC on a group-level. Within pwIIH, those with migraine-like headache had significantly higher pCGRP levels than those with non-migraine-like headache (F(2,524) = 84.79; p < 0.001) and headache absence (F(2,524) = 84.79; p < 0.001) throughout the observation period, explaining 14.7% of the variance in pCGRP levels. CGRP measurements showed strong intraindividual agreement in pwIIH (ICC 0.993, 95% CI 0.987–0.996, p < 0.001). No association was found between pCGRP levels and ophthalmological parameters.
Conclusions.
Although interindividual heterogeneity of pCGRP levels is generally high, migraine-like headache seems to be associated with higher pCGRP levels. CGRP may play a role in the headache pathophysiology at least in a subgroup of IIH.