bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic insights into across pancreatitis types: the causal influence of immunoglobulin G N-glycosylation variants on disease risk
Authors: Chen Yulin , Li Xue , Lu Ran , Lv Yinchun , Ye Junman , Huang Qiaorong , Meng Wentong , Long Feiwu , Burman Jonas , Mo Xianming , Fan Chuanwen 
Published: March 2024

BackgroundWhile a few case-control studies indicated a possible correlation of IgG N-glycosylation patterns with pancreatitis, their restricted sample sizes and methodologies prevented conclusive insights into causality or distinguishing traits across pancreatitis types.MethodWe conducted a two-sample Mendelian Randomization (MR) analysis to investigate the causal relationship between 77 IgG N-glycosylation traits and various types of pancreatitis, including acute pancreatitis (AP), chronic pancreatitis (CP), alcohol acute pancreatitis (AAP), and alcohol chronic pancreatitis (ACP). This analysis utilized summary-level data from genome-wide association studies (GWAS), employing methods such as IVW, MR-Egger, and weighted median. To ensure the robustness of our findings, several sensitivity analyses, including Cochran’s Q statistic, leave-one-out, MR-Egger intercept, and MR-PRESSO global test were conducted.ResultOur study uncovered the causal relationship between specific IgG N-glycosylation traits and various types of pancreatitis. Notably, an increase in genetically predicted IGP7 levels was associated with a decreased risk of developing AP. For CP, our data suggested a protective effect associated with higher levels of both IGP7 and IGP31, contrasting with increased levels of IGP27 and IGP65, which were linked to a heightened risk. Moreover, in the case of AAP, elevated IGP31 levels were causatively associated with a lower incidence, while higher IGP26 levels correlated with an increased risk for ACP.ConclusionThis study establishes causal relationship between specific IgG N-glycosylation patterns and varying risks of different pancreatitis forms, underscoring their potential as predictive biomarkers. These findings necessitate further exploration into the underlying mechanisms, promising to inform more personalized diagnostic and therapeutic strategies in pancreatitis management.
Cultivable microbial diversity, peptide profiles, and bio-functional properties in Parmigiano Reggiano cheese
Authors: Martini Serena , Sola Laura , Cattivelli Alice , Cristofolini Marianna , Pizzamiglio Valentina , Tagliazucchi Davide , Solieri Lisa 
Published: March 2024

IntroductionLactic acid bacteria (LAB) communities shape the sensorial and functional properties of artisanal hard-cooked and long-ripened cheeses made with raw bovine milk like Parmigiano Reggiano (PR) cheese. While patterns of microbial evolution have been well studied in PR cheese, there is a lack of information about how this microbial diversity affects the metabolic and functional properties of PR cheese.MethodsTo fill this information gap, we characterized the cultivable fraction of natural whey starter (NWS) and PR cheeses at different ripening times, both at the species and strain level, and investigated the possible correlation between microbial composition and the evolution of peptide profiles over cheese ripening.Results and discussionThe results showed that NWS was a complex community of several biotypes belonging to a few species, namely, Streptococcus thermophilus, Lactobacillus helveticus, and Lactobacillus delbrueckii subsp. lactis. A new species-specific PCR assay was successful in discriminating the cheese-associated species Lacticaseibacillus casei, Lacticaseibacillus paracasei, Lacticaseibacillus rhamnosus, and Lacticaseibacillus zeae. Based on the resolved patterns of species and biotype distribution, Lcb. paracasei and Lcb. zeae were most frequently isolated after 24 and 30 months of ripening, while the number of biotypes was inversely related to the ripening time. Peptidomics analysis revealed more than 520 peptides in cheese samples. To the best of our knowledge, this is the most comprehensive survey of peptides in PR cheese. Most of them were from β-caseins, which represent the best substrate for LAB cell-envelope proteases. The abundance of peptides from β-casein 38–88 region continuously increased during ripening. Remarkably, this region contains precursors for the anti-hypertensive lactotripeptides VPP and IPP, as well as for β-casomorphins. We found that the ripening time strongly affects bioactive peptide profiles and that the occurrence of Lcb. zeae species is positively linked to the incidence of eight anti-hypertensive peptides. This result highlighted how the presence of specific LAB species is likely a pivotal factor in determining PR functional properties.
Dynamic changes in fungal communities and functions in different air-curing stages of cigar tobacco leaves
Authors: Zhao Songchao , Li Yuanyuan , Liu Fang , Song Zhaopeng , Yang Weili , Lei Yunkang , Tian Pei , Zhao Mingqin 
Published: March 2024

IntroductionAir curing (AC) plays a crucial role in cigar tobacco leaf production. The AC environment is relatively mild, contributing to a diverse microbiome. Fungi are important components of the tobacco and environmental microbiota. However, our understanding of the composition and function of fungal communities in AC remains limited.MethodsIn this study, changes in the chemical constituents and fungal community composition of cigar tobacco leaves during AC were evaluated using flow analysis and high-throughput sequencing.ResultsThe moisture, water-soluble sugar, starch, total nitrogen, and protein contents of tobacco leaves exhibited decreasing trends, whereas nicotine showed an initial increase, followed by a decline. As determined by high-throughput sequencing, fungal taxa differed among all stages of AC. Functional prediction showed that saprophytic fungi were the most prevalent type during the AC process and that the chemical composition of tobacco leaves is significantly correlated with saprophytic fungi.ConclusionThis study provides a deeper understanding of the dynamic changes in fungal communities during the AC process in cigar tobacco leaves and offers theoretical guidance for the application of microorganisms during the AC process.
The protective role of oily fish intake against type 2 diabetes: insights from a genetic correlation and Mendelian randomization study
Authors: Zhang Youqian , Ren Entong , Zhang Chunlong , Wang Yang , Chen Xiaohe , Li Lin 
Published: March 2024

Background and aimsPrevious research has underscored the association between oily fish intake and type 2 diabetes (T2DM), yet the causality remains elusive.MethodsA bidirectional univariable Mendelian Randomization (MR) analysis was employed to evaluate the causal effects of oily fish and non-oily fish intake on T2DM. Replication analysis and meta-analysis were conducted to ensure robust results. Multivariable MR analysis was utilized to assess confounders, and further mediation MR analysis discerned mediating effects. Linkage Disequilibrium Score (LDSC) analysis was undertaken to compute genetic correlations. Inverse variance weighted (IVW) was the primary method, complemented by a series of sensitivity analyses.ResultsThe LDSC analysis unveiled a significant genetic correlation between oily fish intake and T2DM (Genetic correlation: -0.102, p = 4.43 × 10−4). For each standard deviation (SD) increase in genetically predicted oily fish intake, the risk of T2DM was reduced by 38.6% (OR = 0.614, 95% CI 0.504 ~ 0.748, p = 1.24 × 10−6, False Discovery Rate (FDR) = 3.72 × 10−6). The meta-analysis across three data sources highlighted a persistent causal association (OR = 0.728, 95% CI 0.593 ~ 0.895, p = 0.003). No other causal effects were identified (all p &gt; 0.5, FDR &gt; 0.5). The main outcomes remained consistent in most sensitivity analyses. Both MVMR and mediation MR analyses emphasized the mediating roles of triglycerides (TG), body mass index (BMI), and 25-hydroxyvitamin D (25OHD) levels.ConclusionTo encapsulate, there’s an inverse association between oily fish intake and T2DM risk, suggesting potential benefits of oily fish intake in T2DM prevention.
Association between metabolic dysfunction-associated steatotic liver disease and risk of incident pancreatic cancer: a systematic review and meta-analysis of cohort studies
Authors: Zhang  Yi , Zhou Ben-Gang , Zhan Ji-Dong , Du Bin-Bin 
Published: March 2024

Background and objectivesSince the results of previous observational studies on the relationship between metabolic dysfunction-associated steatotic liver disease (MASLD) and pancreatic cancer were still controversial and inconsistent, we performed a systematic evaluation and meta-analysis of cohort studies to assess any potential association.MethodsWe conducted a systematic search of PubMed, Embase, and Web of Science databases from the database’s inception up to November 30, 2023. For summary purposes, hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated using random-effects models, and subgroup and sensitivity analyses were performed as well. The Egger’s test and Begg’s test were utilized to detect the publication bias.ResultsThis meta-analysis included nine cohort studies with a total of 10,428,926 participants. The meta-analysis demonstrated an increased risk of pancreatic cancer in those with MASLD (HR = 1.32, 95% CI: 1.10-1.59, P = 0.003) with moderate heterogeneity (I2 = 54%, P = 0.03). Subsequent subgroup analyses revealed that the pooled HRs remained significantly unchanged, irrespective of the study area, nomenclature of fatty liver disease, and sample size. The results of the sensitivity analyses remained unchanged. No evidence of publication bias was found.ConclusionThis meta-analysis indicated that MASLD was associated with a higher risk of pancreatic cancer. To further strengthen the association, future prospective cohort studies should take into account different ethnic groups, diagnostic methods of fatty liver, the severity of MASLD, and potential confounding factors, as well as explore the potential mechanisms of pancreatic cancer development in MASLD patients.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42023489137.
Post-anthesis dry matter and nitrogen accumulation, partitioning, and translocation in maize under different nitrate–ammonium ratios in Northwestern China
Authors: Wu Bing , Cui Zhengjun , Zechariah Effah , Guo Lizhuo , Gao Yuhong , Yan Bin , Liu Hongsheng , Wang Yifan , Wang Haidi , Li Li 
Published: March 2024

IntroductionAn appropriate supply of ammonium (NH4+) in addition to nitrate (NO3−) can greatly improve plant growth and promote maize productivity. However, knowledge gaps exist regarding the mechanisms by which different nitrogen (N) fertilizer sources affect the enzymatic activity of nitrogen metabolism and non-structural carbohydrates during the post-anthesis period.MethodsA field experiment across 3-year was carried out to explore the effects of four nitrateammonium ratio (NO3−/NH4+ = 1:0 (N1), 1:1 (N2), 1:3 (N3), and 3:1 (N4)) on postanthesis dry matter (DM) and N accumulation, partitioning, transportation, and grain yield in maize.ResultsNO3-/NH4+ ratio with 3:1 improved the enzymatic activity of N metabolism and non-structural carbohydrate accumulation, which strongly promoted the transfer of DM and N in vegetative organs to reproductive organs and improved the pre-anthesis DM and nitrogen translocation efficiency. The enzymatic activities of nitrate reductase, nitrite reductase, glutamine synthetase, glutamine oxoglutarate aminotransferase, and non-structural carbohydrate accumulation under N4 treatment were increased by 9.30%–32.82%, 13.19%–37.94%, 4.11%–16.00%, 11.19%–30.82%, and 14.89%–31.71% compared with the other treatments. Mixed NO3−-N and NH4+-N increased the total DM accumulation at the anthesis and maturity stages, simultaneously decreasing the DM partitioning of stem, increasing total DM, DM translocation efficiency (DMtE), and contribution of pre-anthesis assimilates to the grain (CAPG) in 2015 and 2017, promoting the transfer of DM from stem to grain. Furthermore, the grain yield increased by 3.31%–9.94% (2015), 68.6%–26.30% (2016), and 8.292%–36.08% (2017) under the N4 treatment compared to the N1, N2, and N3 treatments.ConclusionThe study showed that a NO3−/NH4+ ratio of 3:1 is recommended for high-yield and sustainable maize management strategies in Northwestern China.
Analysis of health risk factors for older adults living alone in China and establishment and evaluation of a nomogram prediction model
Authors: Chen Kexin , Qiu Jiangwei , Wang Wenlong , Hu Qi , Qiao Hui 
Published: March 2024

ObjectiveTo understand the health status of older adults living alone in China and analyze the influencing factors, so as to provide reference for improving the health status of older adults living alone.MethodsBased on CGSS data from China General Social Survey (2017), the influencing factors of health status of older adults living alone were analyzed by unconditional Logistic regression, and the R software was used to develop a nomogram for predicting the risk of self-assessed unhealthy adverse outcomes.ResultsGender, annual income, mandarin listening level and participation in medical insurance were the influencing factors of self-rated health of older adults living alone. Age and annual income are the influencing factors of physiological health. Annual income and Internet use were influential factors for mental health. C-Statistic of nomogram prediction model was 0.645. The calibration curve showed that goodness of fit test (χ2 = 58.09, p &lt; 0.001), and the overall prediction ability of the model was good.ConclusionThe health status of older adults living alone in the home-based older adults care is worrying, and it is affected by various factors. We should pay more attention to older adults living alone, improve the ability of listening and distinguishing mandarin and the use of health information platforms for older adults living alone, and further implement medical insurance policies and health services. Announcing the solution to promote healthy home-based care for older adults living alone.
ANALISIS VARIAN ANGGARAN OPERASIONAL PADA PERUMDA TIRTA PAKUAN KOTA BOGOR
Authors: Suprobo Rien Evangelin , Manullang Asna 
Published: March 2024

This research aims to find out whether there are deviati The analytical method used is a descriptive method, namely a research method that only describes the analysis of the company's operational budget so that it can be seen between the budget that has been prepared and agreed with the realization in the field ons between the opeand to find out the causes of operational budget deviations and their realization in the Tirta Pakuan Regional Government of Bogor City rational budget and its realization.	The results of research from the analysis of operational budget variances at Perumda Tirta Pakan, Bogor City, show that there are deviations between income and operational costs, whether unprofitable deviations or profitable deviations. control over revenue is still not optimal (unfavorable) at 0.24 due to the company's targets not being achieved, whereas in terms of operational costs at the Perumda Tirta Pakan company it is effective, this is due to improvements made by the company on any costs where irregularities occur.
keywords: Deviation; operational budget; variance analysis.
PENGARUH DISPLAY PRODUK, KUALITAS PRODUK DAN KELENGKAPAN PRODUK TERHADAP KEPUTUSAN PEMBELIAN KONSUMEN
Authors: Dyanti Anis Nur , Indriyatni Lies 
Published: March 2024

Penelitian ini bertujuan untuk menganalisis display produk, kualitas produk dan kelengkapan produk terhadap keputusan pembelian konsumen. Variable independen dalam penelitian ini adalah display produk, kualitas produk dan kelengkapan produk. Variable dependen dalam penelitian ini adalah keputusan pembelian konsumen. Sumber data dalam penelitian ini yaitu data primer dan sekunder, diperoleh dengan cara menyebarkan kuisioner kepada para responden yaitu para pelanggan Alfamart Pucang Gading. Populasi pada penetilian ini dihitung dari pelanggan Alfamart Pucang Gading. Sampel yang digunakan adalah 97 responden. Teknik pengambilan sampel yang digunakan adalah simple random sampling. Metode analisis data menggunakan program SPSS 25.
Hasilnya menunjukkan bahwa display produk berpengaruh positif dan signifikan terhadap keputusan pembelian konsumen dengan tingkat signifikansi 0.003 t table 1,1661, kualitas produk berpengaruh positif dan signifikan terhadap keputusan pembelian konsumen dengan tingkat signifikasi 0,022 < 0,05 dan t hitung 2,330 > t table 1,661 sedangkan kelengkapan produk tidak berpengaruh positif dan signifikan terhadap keputusan pembelian konsumen dengan tingkat signifikasi 0,315 > 0,05 dan t hitung 1,010 < t table 1,661. Hasil display produk, kualitas produk dan kelengkapan produk secara simultan berpengaruh positif dan signifikan terhadap keputusan pembelian konsumen dengan tingkat signifikansi F hitung 16,717 > F table 2,70.
PENINGKATAN KEWIRAUSAHAAN DALAM PENGOLAHAN PRODUK BAKSO IKAN PATIN
Authors: Khairani Zulia , Soviyanti Efrita , Kamilah Faizah 
Published: March 2024

by the signing of MOU No. 025/FE/Ks/2022 concerning Cooperation in Implementing the Tri Dharma of Higher Education by the Dean of the Faculty of Economics and the Head of Rumbai Bukit Village. Based on the results of previous service activities regarding increasing mothers' knowledge about nutritious food for toddlers, information was found that one of the problems faced by the Rumbai Bukit sub-district was the problem of stunting in children. As a result of the excavation of natural potential in Rumbai Bukit, information was found that there is potential for cassava, papaya, oil palm, catfish, catfish, etc. Based on the completeness of food sources in the Rumbai Bukit sub-district, there should be no under-fives experiencing stunting in this sub-district. This service activity is a continuation of the previous service activity, namely about increasing entrepreneurship in processing catfish meatballs. Patin is a food source that is easy to obtain and at affordable prices in the Riau area, especially the Rumbai Bukit sub-district. The hope is that by increasing mothers' skills in processing nutritious food for children, stunting rates can be reduced and the community's welfare and economy can be improved. The results of this service activity found an increase in community knowledge and skills in processing products made from patin raw materials and as an idea for entrepreneurship

Keywords: products, catfish, entrepreneurship
Umur bibit 25 hari dan bibit kultur jaringan jenis Kappaphycus alvarezii terhadap pertumbuhan dan kandungan karaginan
Authors: Djami Dewiyanti , Ratoe Oedjoe Marcelien Dj , Rebhung Felix 
Published: March 2024

This research was conducted to determine the growth rate and carrageenan content in Kappahycus alvarezii seaweed by comparing the use of 25-day-old selection seeds, unselected seeds and tissue culture seeds. This research was carried out in Oenaek waters for 3 months. observations of growth directly in the cultivation site while for carrageenan content were carried out at the Undana FPKP Laboratory. The results showed that the use of 25-day-old selection seeds can increase the growth rate and carrageenan content in seaweed. The highest absolute growth reached 267.8 grams, while the highest daily growth was 5.146%/gr as well as the carrageenan content which reached 6.32%.

Keywords: Selected seeds aged 25 days, carrageenan content, Kappaphycus alvarezii.
Studi kepadatan ikan sidat (Anguilla Spp) di Sungai Tarus Desa Mata Air Kabupaten Kupang pada bulan September sampai Oktober
Authors: Kefi Rudiyanto , Lukas Ade Yulita Hesti , Santoso Priyo 
Published: March 2024

The research conducted over approximately 60 days at Tarus River, Mata Air Village, Kupang Regency aimed to assess the variety and abundance of eel fish, a high-value commodity in the international market. The study focused on three stations along the river, where the density of eel fish was observed: station 1 (8 individuals), station 2 (5 individuals), and station 3 (2 individuals). Using digital scales, researchers measured the total weight of the caught fish, alongside noting the types of eel fish obtained. The findings, analyzed descriptively and presented through tables and figures, revealed the prevalence of eel fish type A. marmorata during the research period, primarily in the yellow eel and elver phases with varying sizes. Morphometric measurements indicated that Anguilla marmorata specimens typically ranged in total length (35-47 cm), dorsal length (20-30 cm), anal length (15-25 cm), head diameter (5-7 cm), body diameter (7-9 cm), and total weight (170-181 grams). Additionally, water quality assessments at the research site indicated temperatures ranging from 20-27oC, with a pH of 7.5.

Keywords: Identification, eel fish (Anguilla marmorata).
Studi kelimpahan dan morfometrik pada kerang darah (Anadara granosa) di Perairan Motadikin Kabupaten Malaka
Authors: Seran Hendrikus Klau , Santoso Priyo , Linggi Yulianus 
Published: March 2024

Anadara granosa is one of the fisheries commodities that is abundant in various regions, especially tropical areas. The high use of blood cockles by the community in Motadikin waters is considered to result in a decline in the population of blood cockles in nature, thereby increasing population growth. This research aims to examine the abundance and morphometric features of blood cockles in Motadikin waters, Malaka Regency. This research was carried out for 1 month in Motadikin waters, Malacca Regency using the transect method. The number of transects used was 3, the distance between transects was 25 m and each transect used 6 quadrants with a size of 1x1 m and the distance between each quadrant was 5 m. The results of the research showed that the abundance of blood clams in Motadikin Waters was an average of 5-7 individuals/m2 and the average The average abundance found on substrates covered with mangroves (I) is higher than on sandy substrates alone (II). Meanwhile, morphometric measurements of blood clams in Motadikin waters are dominated by blood clams with a length range of 3.2 cm - 3.9 cm, with a shell height of 1.4 cm - 2.7 cm, an umbo height of 1.1 cm - 1.2 cm, shell thickness 2.4 cm - 2.6 cm and ligament length 2.4 cm - 2.7 cm.

Keywords: Anadara granosa, abundance, morphometrics, motadikin waters.
UPAYA MENINGKATKAN HASIL BELAJAR SISWA MENGGUNAKAN METODE PEMBELAJARAN MIND MAPPING PADA MATERI PENGERTIAN DAN MANFAAT PENDAPATAN NASIONAL DI KELAS XI IIS 2 SMA NEGERI 4 KOTA KUPANG TAHUN AJARAN 2021/2022
Authors: Abolladaka Jacob , Abolla Monica Bellandina 
Published: March 2024

Mind mapping (peta pikiran) atau peta konsep merupakan salah satu metode atau cara mengajar yang menumbuhkan kreativitas peserta didik. Dengan metode ini daya ingat siswa mengenai materi dapat lebih lama dan mudah disimpan. Tujuannya penelitian ini untuk mengetahui peninngkatan hasil belajar pada materi pengertian dan manfaat pendapatan nasional, dengan menggunakan teknik deskriptif kualitatif untuk melihat kecendurungan yang terjadi dalam kegiatan pembelajaran. Berdasarkan hasil peneltian pada tahapan siklus I sebanyak 18 orang siswa atau 41% dari 44 orang siwa dinyatakan belum tuntas, hal ini disebabkan penggunakan model yang kurang tepat. Sedangkan siklus II hasil belajar semua siswa dinyatakan tuntas, hal ini menggambarkan dalam proses pembelajaran menggunakan model mind mapping pada siklus II berhasil. Dilihat dari hasil belajar yang diperoleh siswa pada siklus I dan siklus II dapat disimpulkan bahwa dengan menggunakan metode mind mapping dalam mata pelajaran ekonomi dengan materi pengertian dan manfaat pendapatan nasional hasil belajar siswa meningkat.