bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of plateletpheresis on the Fresenius AS.TEC 204 and Haemonetics MCS 3p
Authors: Ranganathan Sudha 
Published: November 2006

AbstractThis is an attempt at comparing two cell separators for plateletpheresis, namely the Fresenius AS.TEC 204 and Haemonetics MCS 3p, at a tertiary care center in India. Donors who weighed between 55–75 kg, who had a hematocrit of 41–43%, and platelet counts of 250 × 103–400 × 103/μl were selected for the study. The comparability of the donors who donated on the two cell separators were analysed by t‐test independent samples and no significant differences were found (P > 0.05). The features compared were time taken for the procedure, volume processed on the separators, adverse reactions of the donors, quality control of the product, separation efficiency of the separators, platelet loss in the donors after the procedure, and the predictor versus the actual yield of platelets given by the cell separator. The volume processed to get a target yield of >3 × 1011 was equal to 2.8–3.2 l and equal in both the cell separators. Symptoms of citrate toxicity were seen in 4 and 2.5% of donors who donated on the MCS 3p and the AS.TEC 204, respectively, and 3 and 1% of donors, respectively, had vasovagal reactions. All the platelet products collected had a platelet count of >3 × 1011; 90% of the platelet products collected on the AS.TEC 204 attained the predicted yield that was set on the cell separator where as 75% of the platelet products collected on the MCS 3p attained the target yield. Quality control of the platelets collected on both the cell separators complied with the standards except that 3% of the platelets collected on the MCS 3p had a visible red cell contamination. The separation efficiency of the MCS 3p was higher, 50–52% as compared to the 40–45% on the AS.TEC 204. A provision of double venous access, less adverse reactions, negligible RBC contamination with a better predictor yield of platelets makes the AS.TEC 204 a safer and more reliable alternative than the widely used Haemonetics MCS 3p. J. Clin. Apheresis. © 2006 Wiley‐Liss, Inc.
Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts
Authors: Sugiki Tadashi , Uyama Taro , Toyoda Masashi , Morioka Hideo , Kume Shoen , Miyado Kenji , Matsumoto Kenji , Saito Hirohisa , Tsumaki Noriyuki , Takahashi Yoriko , Toyama Yoshiaki , Umezawa Akihiro 
Published: November 2006

AbstractWhat is it that defines a bone marrow‐derived chondrocyte? We attempted to identify marrow‐derived cells with chondrogenic nature and immortality without transformation, defining “immortality” simply as indefinite cell division. KUM5 mesenchymal cells, a marrow stromal cell line, generated hyaline cartilage in vivo and exhibited enchondral ossification at a later stage after implantation. Selection of KUM5 chondroblasts based on the activity of the chondrocyte‐specific cis‐regulatory element of the collagen α2(XI) gene resulted in enhancement of their chondrogenic nature. Gene chip analysis revealed that OP9 cells, another marrow stromal cell line, derived from macrophage colony‐stimulating factor‐deficient osteopetrotic mice and also known to be niche‐constituting cells for hematopoietic stem cells expressed chondrocyte‐specific or ‐associated genes such as type II collagen α1, Sox9, and cartilage oligomeric matrix protein at an extremely high level, as did KUM5 cells. After cultured OP9 micromasses exposed to TGF‐β3 and BMP2 were implanted in mice, they produced abundant metachromatic matrix with the toluidine blue stain and formed type II collagen‐positive hyaline cartilage within 2 weeks in vivo. Hierarchical clustering and principal component analysis based on microarray data of the expression of cell surface markers and cell‐type‐specific genes resulted in grouping of KUM5 and OP9 cells into the same subcategory of “chondroblast,” that is, a distinct cell type group. We here show that these two cell lines exhibit the unique characteristics of hyaline cartilage formation and enchondral ossification in vitro and in vivo. J. Cell. Biochem. 100: 1240–1254, 2007. © 2006 Wiley‐Liss, Inc.
Mouse embryonic fibroblast cells from transgenic mice overexpressing tNOX exhibit an altered growth and drug response phenotype
Authors: Yagiz Kader , Wu Lion‐Ying , Kuntz Charles P. , James Morré D. , Morré Dorothy M. 
Published: November 2006

AbstractMouse embryonic fibroblast (MEF) cells prepared from transgenic mice overexpressing a cancer‐specific and growth‐related cell surface NADH oxidase with protein disulfide‐thiol interchange activity grew at rates approximately twice those of wild‐type embryonic fibroblast cells. Growth of transgenic MEF cells overexpressing tNOX was inhibited by low concentrations of the green tea catechin (−)‐epigallocatechin‐3‐gallate (EGCg) or the synthetic isoflavene phenoxodiol. Both are putative tNOX‐targeted inhibitors with anti‐cancer activity. With both EGCg and phenoxodiol, growth inhibition was followed after about 48 h by apoptosis. Growth of wild‐type mouse fibroblast cells from the same strain was unaffected by EGCg and phenoxodiol and neither compound induced apoptosis even at concentrations 100–1,000‐fold higher than those that resulted in apoptotic death in the transgenic MEF cells. The findings validate earlier reports of evidence for tNOX presence as contributing to unregulated growth of cancer cells as well as the previous identification of the tNOX protein as the molecular target for the anti‐cancer activities attributed to both EGCg and phenoxodiol. The expression of tNOX emerges as both necessary and sufficient to account for the cancer cell‐specific growth inhibitions by both EGCg and phenoxodiol. J. Cell. Biochem. 101: 295–306, 2007. © 2006 Wiley‐Liss, Inc.
Pair interaction energy decomposition analysis
Authors: Fedorov Dmitri G. , Kitaura Kazuo 
Published: November 2006

AbstractThe energy decomposition analysis (EDA) by Kitaura and Morokuma was redeveloped in the framework of the fragment molecular orbital method (FMO). The proposed pair interaction energy decomposition analysis (PIEDA) can treat large molecular clusters and the systems in which fragments are connected by covalent bonds, such as proteins. The interaction energy in PIEDA is divided into the same contributions as in EDA: the electrostatic, exchange‐repulsion, and charge transfer energies, to which the correlation (dispersion) term was added. The careful comparison to the ab initio EDA interaction energies for water clusters with 2–16 molecules revealed that PIEDA has the error of at most 1.2 kcal/mol (or about 1%). The analysis was applied to (H2O)1024, the α helix, β turn, and β strand of polyalanine (ALA)10, as well as to the synthetic protein (PDB code 1L2Y) with 20 residues. The comparative aspects of the polypeptide isomer stability are discussed in detail. © 2006 Wiley Periodicals, Inc.J Comput Chem 2007
N‐glycosylation of the Xenopus laevis ClC‐5 protein plays a role in cell surface expression, affecting transport activity at the plasma membrane
Authors: Schmieder Sandra , Bogliolo Stéphanie , Ehrenfeld Jordi 
Published: November 2006

AbstractMutations in the gene encoding ClC‐5 lead to X‐linked hypercalciuric nephrolithiasis (XLHN), characterized by proteinuria, hypercalciuria, and phosphaturia. In renal proximal tubule cells, ClC‐5 was identified as an important player in endocytosis, which ensures reabsorption of filtered protein. However, the recent finding that ClC‐5 is a Cl−/H+ antiporter and not a Cl− channel as long thought points to the lack of understanding of its functional role. Also, little biochemical data are available about ClC‐5 and its post‐translational modifications have not been investigated. Here, we examined the role of N‐glycosylation of xClC‐5 in the Xenopus oocyte expression system by comparing wild‐type (WT) xClC‐5 and N‐glycosylation site mutants. We found that xClC‐5 is N‐glycosylated on asparagines 169 and 470, which are the only N‐glycosylated sites. xClC‐5 mutants have an increased susceptibility to polyubiquitination and proteasomal degradation; however, without a notable impact on the expression level. Using a cross‐linking reagent, we showed that xClC‐5 assembles into protein complexes, independent of its N‐glycosylation. Voltage‐clamp measurements showed a reduced conductance in the presence of tunicamycin and with xClC‐5 N‐glycosylation site mutants. Using immunocytochemistry, we localized xClC‐5 mainly in intracellular compartments, and found that its cell surface pool is reduced in the absence of N‐glycans. We further examined the plasma membrane retrieval of WT and mutant xClC‐5 in the presence of Brefeldin A (BFA), and found that the non‐glycosylated mutant was retrieved more than five times faster than the WT protein. We conclude that N‐glycosylation enhances cell surface expression of xClC‐5, increasing its plasma membrane transport activity. J. Cell. Physiol. 210: 479–488, 2007. © 2006 Wiley‐Liss, Inc.
Patterning the developing and regenerating olfactory system
Authors: Henion Timothy R. , Schwarting Gerald A. 
Published: November 2006

AbstractThe olfactory system is a remarkable model for investigating the factors that influence the guidance of sensory axon populations to specific targets in the CNS. Since the initial discovery of the vast odorant receptor (ORs) gene family in rodents and the subsequent finding that these molecules directly influence targeting, several additional olfactory axon guidance cues have been identified. Two of these, ephrins and semaphorins, have well‐established functions in patterning axon connections in other systems. In addition, lactosamine‐containing glycans are also required for proper targeting and maintenance of olfactory axons, and may also function in other sensory regions. It is now apparent that these and likely other additional molecules are required along with ORs to orchestrate the complex pattern of convergence and divergence that is unique to the olfactory system. J. Cell. Physiol. 210: 290–297, 2007. © 2006 Wiley‐Liss, Inc.
Differentiation of human adult skin‐derived neuronal precursors into mature neurons
Authors: Gingras Marie , Champigny Marie‐France , Berthod François 
Published: November 2006

AbstractThe isolation of autologous neuronal precursors from skin‐derived precursor cells extracted from adult human skin would be a very efficient source of neurons for the treatment of various neurodegenerative diseases. The purpose of this study was to demonstrate that these neuronal precursors were able to differentiate into mature neurons. We isolated neuronal precursors from breast skin and expanded them in vitro for over ten passages. We showed that 48% of these cells were proliferating after the first passage, while this growth rate decreased after the second passage. We demonstrated that 70% of these cells were nestin‐positive after the third passage, while only 17% were neurofilament M‐positive after 7 days of differentiation. These neuronal precursors expressed βIII tubulin, the dendritic marker MAP2 and the presynaptic marker synaptophysin after 7 days of in vitro maturation. They also expressed the postsynaptic marker PSD95 and the late neuronal markers NeuN and neurofilament H after 21 days of differentiation, demonstrating they became terminally differentiated neurons. These markers were still expressed after 50 days of culture. The generation of autologous neurons from an accessible adult human source opens many potential therapeutic applications and has a great potential for the development of experimental studies on normal human neurons. J. Cell. Physiol. 210: 498–506, 2007. © 2006 Wiley‐Liss, Inc.
Mitogen activated protein kinase‐dependent activation of c‐Jun and c‐Fos is required for neuronal differentiation but not for growth and stress response in PC12 cells
Authors: Eriksson Minna , Taskinen Minna , Leppä Sirpa 
Published: November 2006

AbstractMAPK‐dependent activation of AP‐1 protein c‐Jun is involved in PC12 cell differentiation and apoptosis. However, the role of other AP‐1 proteins and their connection to MAPKs during growth, differentiation and apoptosis has remained elusive. Here we studied the activation of AP‐1 proteins in response to ERK, JNK, and p38 signaling upon NGF, EGF and anisomycin exposures. All treatments caused different kinetics and strength of MAPK and AP‐1 activities. NGF induced persistent ERK and AP‐1 activities, whereas upon EGF and anisomycin exposures, their activities were only weakly and transiently induced. The sustained AP‐1 activity was associated with concomitant c‐Fos and c‐Jun expression and phoshorylation, which were JNK and ERK dependent. While inhibition of the ERK, JNK, and p38 activities partially prevented AP‐1 activity and suppressed differentiation, none of them was required for anisomycin‐induced apoptosis. The importance of c‐Fos and c‐Jun as mediators of differentiation was demonstrated by the findings that the corresponding siRNAs suppressed NGF‐induced neurite outgrowth. However, the capacity of c‐Fos to promote differentiation required cooperation with Jun proteins. In contrast, Fra‐2 expression was not required for the differentiation response. Together, the results show that sustained c‐Jun and c‐Fos activities mediate MAPK signaling and are essential for differentiation of PC12 cells. J. Cell. Physiol. 210: 538–548, 2007. © 2006 Wiley‐Liss, Inc.
Activation of the β myosin heavy chain promoter by MEF‐2D, MyoD, p300, and the calcineurin/NFATc1 pathway
Authors: Meissner Joachim D. , Umeda Patrick K. , Chang Kin‐Chow , Gros Gerolf , Scheibe Renate J. 
Published: November 2006

AbstractCalcium is a key element in intracellular signaling in skeletal muscle. Changes in intracellular calcium levels are thought to mediate the fast‐to‐slow transformation of muscle fiber type. One factor implicated in gene regulation in adult muscle is the nuclear factor of activated T‐cells (NFAT) isoform c1, whose dephosphorylation by the calcium/calmodulin‐dependent phosphatase calcineurin facilitates its nuclear translocation. Here, we report that differentiated C2C12 myotubes predominantly expressing fast‐type MyHCII protein undergo fast‐to‐slow transformation following calcium‐ionophore treatment, with several transcription factors and a transcriptional coactivator acting in concert to upregulate the slow myosin heavy chain (MyHC) β promoter. Transient transfection assays demonstrated that the calcineurin/NFATc1 signaling pathway is essential for MyHCβ promoter activation during transformation of C2C12 myotubes but is not sufficient for complete fast MyHCIId/x promoter inhibition. Along with NFATc1, myocyte enhancer factor‐2D (MEF‐2D) and the myogenic transcription factor MyoD transactivated the MyHCβ promoter in calcium‐ionophore‐treated myotubes in a calcineurin‐dependent manner. To elucidate the mechanism involved in regulating MyHCβ gene expression, we analyzed the −2.4‐kb MyHCβ promoter construct forcis‐regulatory elements. Using electrophoretic mobility shift assays (EMSAs), chromatin immunoprecipitation assays (ChIP), and nuclear complex coimmunoprecipitation (NCcoIP) assays, we demonstrated calcium‐ionophore‐induced binding of NFATc1 to a NFAT consensus site adjacent to a MyoD‐binding E‐box. At their respective binding sites, both NFATc1 and MyoD recruited the transcriptional coactivator p300, and in turn, MEF‐2D bound to the MyoD complex. The calcium‐ionophore‐induced effects on the MyHCβ promoter were shown to be calcineurin‐dependent. Together, our findings demonstrate calcium‐ionophore‐induced activation of the β MyHC promoter by NFATc1, MyoD, MEF‐2D, and p300 in a calcineurin‐dependent manner. J. Cell. Physiol. 211: 138–148, 2007. © 2006 Wiley‐Liss, Inc.
Life without water: expression of plant LEA genes by an anhydrobiotic arthropod
Authors: Hand Steven C. , Jones Dana , Menze Michael A. , Witt Trudy L. 
Published: November 2006

AbstractAnhydrobiotic animals protect cellular architecture and metabolic machinery in the dry state, yet the molecular repertoire supporting this profound dehydration tolerance is not fully understood. For the desiccation‐tolerant crustacean, Artemia franciscana, we report differential expression of two distinct mRNAs encoding for proteins that share sequence similarities and structural features with late‐embryogenesis abundant (LEA) proteins originally discovered in plants. Bioinformatic analyses support assignment of the LEA proteins from A. franciscana to group 3. This eucoelomate species is the most highly evolved animal for which LEA gene expression has been reported. It is becoming clear that an ensemble of micromolecules and macromolecules is important for establishing the physical conditions required for cellular stabilization during drying in nature. J. Exp. Zool. 305A:62–66, 2006. © 2006 Wiley‐Liss, Inc.
Nowhere‐zero flows in tensor product of graphs
Authors: Zhang Zhao , Zheng Yirong , Mamut Aygul 
Published: November 2006

AbstractIn this paper, we characterize graphs whose tensor product admit nowhere‐zero 3‐flow. The main result is: For two graphs G1 and G2 with δ ≥ 2 and G2 not belonging to a well‐characterized class of graphs, the tensor product of G1 and G2 admits a nowhere‐zero 3‐flow. © 2006 Wiley Periodicals, Inc. J Graph Theory 54: 284–292, 2007
Multivariate analysis approach to the plasma protein profile of patients with advanced colorectal cancer
Authors: Ragazzi Eugenio , Pucciarelli Salvatore , Seraglia Roberta , Molin Laura , Agostini Marco , Lise Mario , Traldi Pietro , Nitti Donato 
Published: November 2006

AbstractThe aim of the present study was to identify the pattern of plasma protein species of interest as markers of colorectal cancer (CRC). Using matrix‐assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS), the plasma protein profile was determined in nine stage IV CRC patients (study group) and nine clean‐colon healthy subjects (control group). Multivariate analysis methods were employed to identify distinctive disease patterns at protein spectrum. In the study and control groups, cluster analysis (CA) on the complete MALDI‐MS spectra plasma protein profile showed a distinction between CRC patients and healthy subjects, thus allowing the identification of the most discriminating ionic species. Principal component analysis (PCA) and linear discriminant analysis (LDA) yielded similar grouping results. LDA with leave‐one‐out cross validation achieved a correct classification rate of 89% in both the patients and the healthy subjects. Copyright © 2006 John Wiley & Sons, Ltd.
Seasonality of the northern hemisphere circumpolar vortex
Authors: Wrona Kalyn M. , Rohli Robert V. 
Published: November 2006

AbstractIn previous research, Rohli et al. (2005) identified long‐term features of the northern hemispheric circumpolar vortex (NHCPV) in January. This research provides a seasonal analysis using December and February to augment the previously analyzed January data in representing winter, along with April, July, and October data to represent spring, summer, and autumn, respectively. A representative 500 hPa geopotential height contour was selected to delineate the NHCPV in each of the five months. The area, shape, and centroid of the monthly December, February, April, July, and October NHCPV are computed for 1959–2001 to supplement the previously identified January properties. These geometrical features of the NHCPV reveal relationships between hemispheric‐scale circulation and temperature anomalies throughout the year. A circularity ratio (Rohli et al., 2005) is used to characterize the shape of the hemispheric‐scale circulation. Results suggest that only October exhibit long‐term trends in either area or circularity, with July being the most variable month in area and October being the most variable month in circularity. Centroids tend to be skewed toward the Pacific basin, except in spring, but few systematic temporal shifts in centroid position were noted for any month. The NHCPV is correlated with atmospheric teleconnection patterns in several months. For example, as was the case for January (Rohli et al., 2005), the Arctic Oscillation (AO) is associated with the area of the December, February, and April NHCPV, while in December the circularity is positively correlated to the AO Index. Also, the Pacific‐North American index is correlated with the area of the December and February NHCPV and with the shape of the December and October NHCPV. Copyright © 2006 Royal Meteorological Society
Measuring and managing engagement in a cross‐cultural workforce: New insights for global companies
Authors: Sanchez Paul , McCauley Dan 
Published: November 2006

AbstractShould a company take a global or local approach to building engagement in its worldwide workforce? Research by Mercer Human Resource Consulting finds that country norms for drivers of engagement, as well as for other employee perceptions about their work and their employers, differ noticeably from region to region—and not in ways that might be expected. But a core set of drivers is emerging globally. This should help multinational companies distinguish between global and local engagement issues, design a manageable global engagement strategy and initiatives, and tailor implementation to meet the needs and preferences of the local workforce. © 2006 Wiley Periodicals, Inc.
Critical events and next best activities in relationship marketing: Best practices beyond advanced analytics
Authors: Bartikowski Boris , Braunmüller Peter 
Published: November 2006

AbstractCustomer relationship management (CRM) systems are the backbone of effective marketing efforts. Yet some research has found that such systems can suffer failure rates as high as 75 percent, thus negating their value to their organizations. This interview explores both the promise and the problems of CRM applications. Because effective CRM implementation requires thorough organizational integration, particularly in the use of data warehousing techniques, both academic and managerial perspectives are brought to bear as the discussants detail the evolution of CRM applications and where their challenges lie. And they highlight some best practices of eventtriggered CRM uses. © 2006 Wiley Periodicals, Inc.
Stimulation of EP4 receptor enhanced bone consolidation during distraction osteogenesis
Authors: Chang Fei , Mishima Hajime , Ishii Tomoo , Yanai Takaji , Akaogi Hiroshi , Sakai Shinsuke , Yoshioka Tomokazu , Ochiai Naoyuki 
Published: November 2006

AbstractThe objective of this study was to confirm whether an agonist of prostaglandin E receptor subtype EP4 can enhance bone consolidation in distraction osteogenesis. A rat distraction osteogenesis model was generated. A unilateral external fixator was fixed to the left femur of the rats of this model after osteotomy. Seven days later, 0.25 mm/12 h or 0.5 mm/12 h elongation was performed for 2 weeks. A systemic administration of an EP4 receptor agonist (ONO 4819 · CD, 3, 10, 30 µg/kg) or normal saline by subcutaneous injection was also performed for 2 weeks. The animals were sacrificed 10, 14, 17, 21, and 42 days after the operation. Radiographic examination, histological examination, and measurements of bone mineral density (BMD) and distraction‐callus hardness were performed to qualitatively and quantitatively evaluate new bone formation. Twenty‐one days after the operation, the experimental group had a higher BMD and a higher distraction‐callus hardness than that of the control group. Forty‐two days after the operation, BMD was similar among all of the groups. But the hardness of the experimental groups increased more than that of the control group, so the statistical differences in distraction‐callus hardness became more distinct between the two groups, indicating an improved remodeling of the distraction callus. These findings are also supported by histological examination. Subcutaneous injection of an EP4 receptor agonist can promote bone formation and remodeling during distraction osteogenesis. ONO 4819 · CD might be a potential candidate for shortening the treatment time of distraction osteogenesis. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:221–229, 2007