bluuebunny/crossref_metadata_2025_split huggingface dataset:
The LysM Receptor-Like Kinase SlLYK10 Controls Lipochitooligosaccharide Signaling in Inner Cell Layers of Tomato Roots
Authors: Ding Yi , Wang Tongming , Gasciolli Virginie , Reyt Guilhem , Remblière Céline , Marcel Fabien , François Tracy , Bendahmane Abdelhafid , He Guanghua , Bono Jean Jacques , Lefebvre Benoit 
Published: April 2024

Abstract
Establishment of arbuscular mycorrhiza relies on a plant signaling pathway that can be activated by fungal chitinic signals such as short-chain chitooligosaccharides and lipo-chitooligosaccharides (LCOs). The tomato LysM receptor-like kinase SlLYK10 has high affinity for LCOs and is involved in root colonization by arbuscular mycorrhizal fungi (AMF); however, its role in LCO responses has not yet been studied. Here, we show that SlLYK10 proteins produced by the Sllyk10-1 and Sllyk10-2 mutant alleles, which both cause decreases in AMF colonization and carry mutations in LysM1 and 2, respectively, have similar LCO-binding affinities compared to the WT SlLYK10. However, the mutant forms were no longer able to induce cell death in Nicotiana benthamiana when co-expressed with MtLYK3, a Medicago truncatula LCO co-receptor, while they physically interacted with MtLYK3 in co-purification experiments. This suggests that the LysM mutations affect the ability of SlLYK10 to trigger signaling through a potential co-receptor rather than its ability to bind LCOs. Interestingly, tomato lines that contain a calcium (Ca2+) concentration reporter [genetically encoded Ca2+ indicators (GECO)], showed Ca2+ spiking in response to LCO applications, but this occurred only in inner cell layers of the roots, while short-chain chitooligosaccharides also induced Ca2+ spiking in the epidermis. Moreover, LCO-induced Ca2+ spiking was decreased in Sllyk10-1*GECO plants, suggesting that the decrease in AMF colonization in Sllyk10-1 is due to abnormal LCO signaling.
The AGAMOUS-LIKE 16–GENERAL REGULATORY FACTOR 1 module regulates axillary bud outgrowth via catabolism of abscisic acid in cucumber
Authors: Chen Jiacai , Liu Liu , Wang Guanghui , Chen Guangxin , Liu Xiaofeng , Li Min , Han Lijie , Song Weiyuan , Wang Shaoyun , Li Chuang , Wang Zhongyi , Huang Yuxiang , Gu Chaoheng , Yang Zhengan , Zhou Zhaoyang , Zhao Jianyu , Zhang Xiaolan 
Published: April 2024

Abstract
Lateral branches are important components of shoot architecture and directly affect crop yield and production cost. Although sporadic studies have implicated abscisic acid (ABA) biosynthesis in axillary bud outgrowth, the function of ABA catabolism and its upstream regulators in shoot branching remain elusive. Here, we showed that the MADS-box transcription factor AGAMOUS-LIKE 16 (CsAGL16) is a positive regulator of axillary bud outgrowth in cucumber (Cucumis sativus). Functional disruption of CsAGL16 led to reduced bud outgrowth, whereas overexpression of CsAGL16 resulted in enhanced branching. CsAGL16 directly binds to the promoter of the ABA 8′-hydroxylase gene CsCYP707A4 and promotes its expression. Loss of CsCYP707A4 function inhibited axillary bud outgrowth and increased ABA levels. Elevated expression of CsCYP707A4 or treatment with an ABA biosynthesis inhibitor largely rescued the Csagl16 mutant phenotype. Moreover, cucumber General Regulatory Factor 1 (CsGRF1) interacts with CsAGL16 and antagonizes CsAGL16-mediated CsCYP707A4 activation. Disruption of CsGRF1 resulted in elongated branches and decreased ABA levels in the axillary buds. The Csagl16 Csgrf1 double mutant exhibited a branching phenotype resembling that of the Csagl16 single mutant. Therefore, our data suggest that the CsAGL16–CsGRF1 module regulates axillary bud outgrowth via CsCYP707A4-mediated ABA catabolism in cucumber. Our findings provide a strategy to manipulate ABA levels in axillary buds during crop breeding to produce desirable branching phenotypes.
The effect of local anesthetic on the hypersensitive and nonsensitive areas of the penis is different in primary premature ejaculation: a pilot study
Authors: Zheng Lei , Wei Le-Tian , Song-Chunli  , Liu Wen-Rong , Jiang Hui , Jiang Tao 
Published: April 2024

Abstract

Background
Penile hypersensitivity is not the whole penis, but rather only a part of the penis. Though local anesthetic can prolong intravaginal ejaculation latency time by reducing penile hypersensitivity, the effect on the hypersensitive and nonsensitive areas of penis is still unclear.

Aim
The study aimed to explore whether the effect of local anesthetic on the hypersensitive and nonsensitive areas of the penis is different in premature ejaculation.

Methods
Penile neurophysiological tests were performed on 290 patients with primary premature ejaculation. The sensory threshold, latency, and amplitude were recorded before and after the topical application of a local anesthetic (lidocaine cream) on the penis.

Outcomes
Local anesthetics increased the sensory thresholds of hypersensitive and nonsensitive areas of the penis without difference but only prolonged the latency of the hypersensitive areas.

Results
According to the neurophysiological results, 149 of 290 patients with primary premature ejaculation had normal penile sensitivity and 141 had penile hypersensitivity. While penile hypersensitivity does not necessarily mean that the whole penis is hypersensitive, and may be that only a part of the penis is hypersensitive, and we examined the following hypersensitivities: glans hypersensitivity only (14 cases), shaft hypersensitivity only (77 cases), and whole penis hypersensitivity (50 cases). Local anesthetics (lidocaine cream) increased the sensory thresholds of hypersensitive and nonsensitive areas of the penis without difference (P &lt; .001) but only prolonged the latency of the hypersensitive areas (P &lt; .001), and the latency of the nonsensitive areas was not different (P &gt; .05).

Clinical Implications
The present discovery implies that it is possible to improve ejaculation by applying local anesthetics externally to the hypersensitive areas of the penis to reduce the afferent local sensory signals, and improve intravaginal ejaculation latency time through accurately decreasing penile sensibility.

Strengths & Limitations
This is the first large-sample study to explore the difference of local anesthetics’ effects on the hypersensitive and nonsensitive areas of the penis by means of neurophysiological methods in premature ejaculation. Our study exclusively examines alterations in penile evoked potential following electrical stimulation, which may not entirely encompass shifts in penile receptivity during sexual activity.

Conclusion
The effects of local anesthetics on the same penis varied with penile sensitivity, and can only prolong the latency of hypersensitive area of the penis. The effect of local anesthetic on the hypersensitive and nonsensitive areas of the penis is different in premature ejaculation.
Sexual health among the oldest old: a population-based study among people aged 85 years and older in Stockholm, Sweden
Authors: Politis Marios , Kosidou Kyriaki , Fors Stefan , Nord Martina 
Published: April 2024

Abstract

Background
Sexual health is an important contributor to the well-being and life satisfaction of people aged ≥85 years, known as the oldest old. However, little is known about sexual health in this population.

Aim
To examine aspects of sexual health among the oldest old and explore its associations with sociodemographic, health-related, and lifestyle factors.

Methods
We conducted a population-based cross-sectional study including 183 individuals aged ≥85 years who were residents in Stockholm County, Sweden. Responders (response rate, 63%) were interviewed on a range of health, sociodemographic, and lifestyle parameters, including aspects of sexual health. Participants’ responses to the sexual health items were reported as proportions with 95% CIs. Associations were examined with multivariable logistic regression.

Outcomes
We examined sexual activity, sexual satisfaction, problems related to sexual health, and inquiries on sexual health by a health care provider.

Results
Twelve percent of participants (95% CI, 8%-17.6%) were sexually active, and 63.9% (95% CI, 56.5%-70.9%) were satisfied with their sexual lives during the past year. A third (35%; 95% CI, 28.4%-42.2%) reported at least a problem related to sexual health. Only 2.2% (95% CI, 0.6%-5.5%) were asked about sexual health by a health care provider, while 8.2% (95% CI, 4.7%-13.2%) identified a need for such an assessment. Yet, 85.2% (95% CI, 79.3%-90.0%) indicated no need for their sexual health to be evaluated by a health care provider. Being partnered was positively associated with sexual activity (adjusted odds ratio, 9.13; 95% CI, 2.53-32.90), whereas having strong social support was positively associated with being satisfied with one’s sexual life (adjusted odds ratio, 2.96; 95% CI, 1.53-5.74).

Clinical Implications
Health care providers should be proactive in assessing the sexual health of the oldest individuals.

Strengths and Limitations
A representative sample of an underresearched population was used in this study. However, the generalizability of our findings may be restricted due to the small sample. To maintain statistical power from a relatively small sample, we might have lost explanatory power. Given the observational cross-sectional nature of the data, we cannot draw causal inferences based on the observed associations.

Conclusions
A 10th of participants were sexually active, and the majority were satisfied with their sexual lives. Although many participants reported problems related to sexual health, few expressed the need to discuss sexual health with health care providers. Future studies should explore potential barriers to addressing sexual health and unmet health care needs among the oldest old.
High pathogenic avian influenza A(H5) viruses of clade 2.3.4.4b in Europe—Why trends of virus evolution are more difficult to predict
Authors: Fusaro Alice , Zecchin Bianca , Giussani Edoardo , Palumbo Elisa , Agüero-García Montserrat , Bachofen Claudia , Bálint Ádám , Banihashem Fereshteh , Banyard Ashley C , Beerens Nancy , Bourg Manon , Briand Francois-Xavier , Bröjer Caroline , Brown Ian H , Brugger Brigitte , Byrne Alexander M P , Cana Armend , Christodoulou Vasiliki , Dirbakova Zuzana , Fagulha Teresa , Fouchier Ron A M , Garza-Cuartero Laura , Georgiades George , Gjerset Britt , Grasland Beatrice , Groza Oxana , Harder Timm , Henriques Ana Margarida , Hjulsager Charlotte Kristiane , Ivanova Emiliya , Janeliunas Zygimantas , Krivko Laura , Lemon Ken , Liang Yuan , Lika Aldin , Malik Péter , McMenamy Michael J , Nagy Alexander , Nurmoja Imbi , Onita Iuliana , Pohlmann Anne , Revilla-Fernández Sandra , Sánchez-Sánchez Azucena , Savic Vladimir , Slavec Brigita , Smietanka Krzysztof , Snoeck Chantal J , Steensels Mieke , Svansson Vilhjálmur , Swieton Edyta , Tammiranta Niina , Tinak Martin , Van Borm Steven , Zohari Siamak , Adlhoch Cornelia , Baldinelli Francesca , Terregino Calogero , Monne Isabella 
Published: April 2024

Abstract
Since 2016, A(H5Nx) high pathogenic avian influenza (HPAI) virus of clade 2.3.4.4b has become one of the most serious global threats not only to wild and domestic birds, but also to public health. In recent years, important changes in the ecology, epidemiology, and evolution of this virus have been reported, with an unprecedented global diffusion and variety of affected birds and mammalian species. After the two consecutive and devastating epidemic waves in Europe in 2020–2021 and 2021–2022, with the second one recognized as one of the largest epidemics recorded so far, this clade has begun to circulate endemically in European wild bird populations. This study used the complete genomes of 1,956 European HPAI A(H5Nx) viruses to investigate the virus evolution during this varying epidemiological outline. We investigated the spatiotemporal patterns of A(H5Nx) virus diffusion to/from and within Europe during the 2020–2021 and 2021–2022 epidemic waves, providing evidence of ongoing changes in transmission dynamics and disease epidemiology. We demonstrated the high genetic diversity of the circulating viruses, which have undergone frequent reassortment events, providing for the first time a complete overview and a proposed nomenclature of the multiple genotypes circulating in Europe in 2020–2022. We described the emergence of a new genotype with gull adapted genes, which offered the virus the opportunity to occupy new ecological niches, driving the disease endemicity in the European wild bird population. The high propensity of the virus for reassortment, its jumps to a progressively wider number of host species, including mammals, and the rapid acquisition of adaptive mutations make the trend of virus evolution and spread difficult to predict in this unfailing evolving scenario.
Integrative taxonomy supports the establishment of a new deep-sea family of Tanaidacea (Peracarida)
Authors: Błażewicz Magdalena , Jakiel Aleksandra , Bird Graham J , Studzian Maciej 
Published: April 2024

Abstract
The tanaidacean family Colletteidae is large and exhibits highly diverse morphologies. A significant structural feature, the cephalothorax-cheliped attachment, is one of the sources of this variation but is still poorly understood or resolved in phylogenetic terms. The presence (and wide intrafamily disparities) of a lateral carapacial sclerite associated with this articulation is the main contributor to the diversity and this is investigated by us and shown to have valuable information for paratanaoidean phylogenies. We used integrative taxonomy to evaluate the status of several described colletteid genera, Caudalonga, Macrinella and Pseudoarthrura, based on analysis of COI and 18S genes, comparison with molecular data from ten other paratanaoidean families and detailed morphological study including confocal imaging of whole specimens, with the ‘sclerite’ a prime target for analysis. The results supported the establishment of a new family, Caudalongidae fam. nov., extracted from the overburdened Colletteidae, the narrow-bodied taxa defined principally by the small cheliped sclerite partly covered by a wide membrane and a short one-articled uropodal exopod. Apart from redescriptions/redefinitions of the genera Caudalonga, Macrinella, and Pseudoarthrura, two new genera Facultatotanais gen. nov. and Universitatotanais gen. nov. are erected and six new species are described, Caudalonga kurilensis sp. nov., F. bios sp. nov., U. freie sp. nov., U.gandavensis sp. nov., U. harvardi sp. nov., and U. jagiellonensis sp. nov. The Clarion Clipperton-Fracture Zone appears to support a high diversity of the family.
Proteome characterization of liver–kidney comorbidity after microbial sepsis
Authors: Gui Yuan , Yu Yanbao , Wang Wenjia , Wang Yuanyuan , Lu Hanyue , Mozdzierz Sarah , Eskander Kirollos , Lin Yi‐Han , Li Hanwen , Tian Xiao‐Jun , Liu Silvia , Zhou Dong 
Published: April 2024

AbstractSepsis is a life‐threatening condition that occurs when the body responds to an infection but subsequently triggers widespread inflammation and impaired blood flow. These pathologic responses can rapidly cause multiple organ dysfunction or failure either one by one or simultaneously. The fundamental common mechanisms involved in sepsis‐induced multiple organ dysfunction remain unclear. Here, employing quantitative global and phosphoproteomics, we examine the liver's temporal proteome and phosphoproteome changes after moderate sepsis induced by cecum ligation and puncture. In total, 4593 global proteins and 1186 phosphoproteins according to 3275 phosphosites were identified. To characterize the liver–kidney comorbidity after sepsis, we developed a mathematical model and performed cross‐analyses of liver and kidney proteome data obtained from the same set of mice. Beyond immune response, we showed the commonly disturbed pathways and key regulators of the liver–kidney comorbidity are linked to energy metabolism and consumption. Our data provide open resources to understand the communication between the liver and kidney as they work to fight infection and maintain homeostasis.
Proactive distractor suppression in early visual cortex
Authors: Richter David , van Moorselaar Dirk , Theeuwes Jan 
Published: April 2024

AbstractAvoiding distraction by salient yet irrelevant stimuli is critical when accomplishing daily tasks. One possible mechanism to accomplish this is by suppressing stimuli that may be distracting such that they no longer compete for attention. While the behavioral benefits of distractor suppression are well-established, its neural underpinnings are not yet fully understood. In an fMRI study, we examined whether and how sensory responses in early visual areas show signs of distractor suppression after incidental learning of spatial statistical regularities. Participants were exposed to an additional singleton task where, unbeknownst to them, one location more frequently contained a salient distractor. We analyzed whether visual responses in terms of fMRI BOLD were modulated by this distractor predictability. Our findings indicate that implicit spatial priors shape sensory processing even at the earliest stages of cortical visual processing, evident in early visual cortex as a suppression of stimuli at locations which frequently contained distracting information. Notably, while this suppression was spatially (receptive field) specific, it did extend to nearby neutral locations, and occurred regardless of whether distractors, nontarget items or targets were presented at this location, suggesting that suppression arises before stimulus identification. Crucially, we observed similar spatially specific neural suppression even if search was only anticipated, but no search display was presented. Our results highlight proactive modulations in early visual cortex, where potential distractions are suppressed preemptively, before stimulus onset, based on learned expectations. Combined, our study underscores how the brain leverages implicitly learned prior knowledge to optimize sensory processing and attention allocation.
Ketamine metabolism via hepatic CYP450 isoforms contributes to its sustained antidepressant actions
Authors: Nguyen Thi Mai Loan , Guilloux Jean-Philippe , Defaix Céline , Mendez-David Indira , Etting Isabelle , Alvarez Jean-Claude , McGowan Josephine C , Highland Jaclyn N. , Zanos Panos , Lovett Jacqueline , Moaddel Ruin , Corruble Emmanuelle , David Denis J. , Gould Todd D. , Denny Christine A. , Gardier Alain M. 
Published: April 2024

ABSTRACT(R,S)-ketamine (ketamine) has rapid and sustained antidepressant (AD) efficacy at sub-anesthetic doses in depressed patients. A metabolite of ketamine, including (2R,6R)-hydroxynorketamine ((6)-HNKs) has been reported to exert antidepressant actions in rodent model of anxiety/depression. To further understand the specific role of ketamine’s metabolism in the AD actions of the drug, we evaluated the effects of inhibiting hepatic cytochrome P450 enzymes on AD responses. We assessed whether pre-treatment with fluconazole (10 and 20 mg/kg, i.p.) 1 hour prior to ketamine or HNKs (10 mg/kg, i.p.) administration would alter behavioral and neurochemical actions of the drugs in male BALB/cJ mice with a highly anxious phenotype. Extracellular microdialysate levels of glutamate and GABA (Gluext, GABAext) were also measured in the medial prefrontal cortex (mPFC). Pre-treatment with fluconazole altered the pharmacokinetic profile of ketamine, by increasing both plasma and brain levels of ketamine and (R,S)-norketamine, while robustly reducing those of (6)-HNKs. At 24 hours post-injection (t24h), fluconazole prevented the sustained AD-like response of ketamine responses in the forced swim test and splash test, as well as the enhanced cortical GABA levels produced by ketamine. A single (2R,6R)-HNK administration selectively rescued the antidepressant-like activity of ketamine in mice pretreated with fluconazole within 24 hours of treatment. Overall, these findings are consistent with an essential role of (6)-HNK in mediating the sustained antidepressant-like effects of ketamine and suggest potential interactions between pharmacological CYPIs and ketamine during antidepressant treatment in patients.
Endogenous hydrogen peroxide positively regulates secretion of a gut-derived peptide in neuroendocrine potentiation of the oxidative stress response inC. elegans
Authors: Jia Qi , Young Drew , Zhang Qixin , Sieburth Derek 
Published: April 2024

AbstractThe gut-brain axis mediates bidirectional signaling between the intestine and the nervous system and is critical for organism-wide homeostasis. Here we report the identification of a peptidergic endocrine circuit in which bidirectional signaling between neurons and the intestine potentiates the activation of the antioxidant response inC. elegansin the intestine. We identify a FMRF-amide-like peptide, FLP-2, whose release from the intestine is necessary and sufficient to activate the intestinal oxidative stress response by promoting the release of the antioxidant FLP-1 neuropeptide from neurons. FLP-2 secretion from the intestine is positively regulated by endogenous hydrogen peroxide (H2O2) produced in the mitochondrial matrix bysod-3/superoxide dismutase, and is negatively regulated byprdx-2/peroxiredoxin, which depletes H2O2in both the mitochondria and cytosol. H2O2promotes FLP-2 secretion through the DAG and calcium-dependent protein kinase C family memberpkc-2and by the SNAP25 family memberaex-4in the intestine. Together, our data demonstrate a role for intestinal H2O2in promoting inter-tissue antioxidant signaling through regulated neuropeptide-like protein exocytosis in a gut-brain axis to activate the oxidative stress response.
B cell αv integrin regulates germinal center derived lung-resident IgA B cell responses following influenza virus infection
Authors: Montiel-Armendariz Andrea , Roe Kelsey , Lagos-Orellana Jonathan , Martinez-Castro Laura Veronica , Lacy-Hulbert Adam , Acharya Mridu 
Published: April 2024

AbstractEmerging studies have highlighted the importance of tissue-resident B cells in the lungs, for protective immunity against respiratory viruses. However, the mechanisms controlling generation and maintenance of such tissue-resident B cells at respiratory sites remain obscure. We have previously shown that αv integrins limit B cell responses to antigens containing Toll-like receptor ligands, and that deletion of B cell αv integrins, in mice, enhances germinal center (GC)-derived long-lived B cell responses after systemic immunization with viral antigens. Here we investigated whether αv also regulates B cell responses at the respiratory tract during viral infection. Our data show that αv integrin restricts tissue-resident B cell responses in the airway, and that deletion of B cell αv promotes generation of lung-resident IgA B cell responses following influenza A virus (IAV) infection. Investigating the mechanism for this, we found that loss of B cell αv, promotes persistence of GC reactions locally in the lungs, which leads to increases in lung-resident IgA+ memory B cells, cross-reactive to antigenic variants. Thus, these studies reveal how IgA B cells are maintained in the lungs and point to a new strategy to improve the durability of lung-resident IgA B cell responses for IAV vaccine efficacy.