bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile
Authors: Gálvez-Silva Matías , Arros Patricio , Berríos-Pastén Camilo , Villamil Aura , Rodas Paula I. , Araya Ingrid , Iglesias Rodrigo , Araya Pamela , Hormazábal Juan C. , Bohle Constanza , Chen Yahua , Gan Yunn-Hwen , Chávez Francisco P. , Lagos Rosalba , Marcoleta Andrés E. 
Published: March 2024

Abstract
Background
The convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global health concern. Hypervirulent K. pneumoniae (hvKp) strains, frequently from sequence type 23 (ST23) and having a K1 capsule, have been associated with severe community-acquired invasive infections. Although hvKp were initially restricted to Southeast Asia and primarily antibiotic-sensitive, carbapenem-resistant hvKp infections are reported worldwide. Here, within the carbapenemase production Enterobacterales surveillance system headed by the Chilean Public Health Institute, we describe the isolation in Chile of a high-risk ST23 dual-carbapenemase-producing hvKp strain, which carbapenemase genes are encoded in a single conjugative plasmid.

Results
Phenotypic and molecular tests of this strain revealed an extensive resistance to at least 15 antibiotic classes and the production of KPC-2 and VIM-1 carbapenemases. Unexpectedly, this isolate lacked hypermucoviscosity, challenging this commonly used hvKp identification criteria. Complete genome sequencing and analysis confirmed the K1 capsular type, the KpVP-1 virulence plasmid, and the GIE492 and ICEKp10 genomic islands carrying virulence factors strongly associated with hvKp. Although this isolate belonged to the globally disseminated hvKp clonal group CG23-I, it is unique, as it formed a clade apart from a previously reported Chilean ST23 hvKp isolate and acquired an IncN KPC-2 plasmid highly disseminated in South America (absent in other hvKp genomes), but now including a class-I integron carrying blaVIM−1 and other resistance genes. Notably, this isolate was able to conjugate the double carbapenemase plasmid to an E. coli recipient, conferring resistance to 1st -5th generation cephalosporins (including combinations with beta-lactamase inhibitors), penicillins, monobactams, and carbapenems.

Conclusions
We reported the isolation in Chile of high-risk carbapenem-resistant hvKp carrying a highly transmissible conjugative plasmid encoding KPC-2 and VIM-1 carbapenemases, conferring resistance to most beta-lactams. Furthermore, the lack of hypermucoviscosity argues against this trait as a reliable hvKp marker. These findings highlight the rapid evolution towards multi-drug resistance of hvKp in Chile and globally, as well as the importance of conjugative plasmids and other mobile genetic elements in this convergence. In this regard, genomic approaches provide valuable support to monitor and obtain essential information on these priority pathogens and mobile elements.
Interdisciplinary all-on-four® concept for mandibular jaw in dental education - do students benefit from individual 3d printed models from real patient cases?
Authors: Bjelopavlovic Monika , Goetze Elisabeth , Kämmerer Peer W , Scheller Herbert 
Published: March 2024

Abstract
Purpose
Digitalization is assuming increasing significance in dental education, as dental students are increasingly exposed to digital implant planning and contemporary technologies such as 3D printing. In this study, we present a cohort analysis aimed at assessing the potential benefits derived from the utilization of 3D prints to seamlessly translate planned procedures into real-life applications.

Methods
21 dental students participated in a virtual planning and hands-on course across two cohorts (C1: n = 10, C2: n = 11). The virtual implant planning phase involved the placement of four implants on an atrophic lower jaw model. Subsequently, Cohort 1 (C1) executed the implantation procedure on a prefabricated hands-on model, while Cohort 2 (C2) engaged with 3D prints representing their individual implant planning during the hands-on session. Subjective assessments of knowledge, skills, and the perceived utility of 3D prints were conducted through pre- and post-course questionnaires, utilizing a 5-point scale.

Results
In the subjective evaluation, 17 out of 21 participants expressed a positive appraisal of the use of personalized models. Notably, there was no statistically significant improvement in overall knowledge scores; however, there was a discernible increase of 0.5 points in the ratings related to perceived expertise and procedural abilities.

Conclusion
While there was a notable increase in the subjective ratings of knowledge and abilities, no statistically significant difference was observed between the two groups. The consensus among dental students is that individually planned and printed implant models serve as a valuable and effective tool in hands-on courses.
Modeling, design and optimization of integrated renewable energy systems for electrification in remote communities
Authors: Qiu Kuanrong , Entchev Evgueniy 
Published: March 2024

AbstractIntegrated renewable energy systems are becoming a promising option for electrification in remote communities. Integrating multiple renewable energy sources allows the communities to counteract the weaknesses of one renewable energy source with the strengths of another. This study aims to model, design and optimize integrated renewable energy systems consisting of solar photovoltaic (PV) panels, wind turbines, a biomass power generator, and storage batteries for applications in remote communities in Canada. Biomass is used as a fuel to produce electricity during periods when solar power and wind power are not capable of meeting the power demand. A methodology is developed to optimize the integrated renewable energy systems design, with the aim of minimizing the net present cost (NPC) and the levelized cost of electricity (LCOE) of the energy systems. Results show that the NPC is $3.61 M and the LCOE is $0.255/kWh for an optimized integrated renewable energy system in a sample remote community that has a peak power consumption of 238.7 kW and an average load demand of 2230 kWh/day. Through the present research, the integrated energy systems are evidenced to be an effective option for electrification in remote communities.
Exploring the nutritional landscape and emerging trends in kidney stone research: visualization and bibliometric analysis
Authors: Zyoud Sa’ed H. , Abushamma Faris , Salameh Husam , Abushanab Amani S. , Koni Amer , Abu Taha Adham , Al-Jabi Samah W. , Shahwan Moyad , Jairoun Ammar A. , Shakhshir Muna H. 
Published: March 2024

Abstract
Background
Kidney stones, or renal nephrolithiasis, are common and dangerous, increasing the risk of chronic kidney disease. Dietary manipulation is essential for the prevention and recurrence of kidney stones. Research into nutrition and kidney stones lacks library analysis, making it difficult to identify new trends. This bibliometric study was conducted to explore the current landscape of research on nutrition and kidney stones worldwide. This paper also emphasizes significant research trends in this area over the past two decades, aiming to assist researchers in understanding the current research status and identifying potential future directions.

Methods
The scholarly literature pertaining to diet and kidney stones was systematically explored utilizing SciVerse Scopus to identify pertinent research articles released from 2003 to 2022. The examination encompassed an assessment of publication patterns, key contributors, focal areas of research, prevalent themes, influential articles, and emerging research avenues. Following data extraction to Excel, analyses, including frequencies, percentages, and linear regression, were conducted. Visual exploration was facilitated through the use of the VOSviewer program version 1.6.19.

Results
Between 2003 and 2022, 697 publications on nutrition and kidney stones were identified. There were 478 original articles (68.58%), 120 reviews (17.22%) and 99 other types of publications (14.20%). The United States is the most productive country, with significant growth in research in the fields of nutrition and kidney stones. The United States has demonstrated the strongest partnerships between researchers’ networks from various countries. Cluster analysis revealed three major research themes: sex differences in kidney stone disease, the correlation between increased dietary acid consumption and calcium oxide kidney stones, and nephrolithiasis associated with a ketogenic diet.

Conclusions
This study offers a thorough examination of nutrition and kidney stone research, encompassing key research domains, collaborative networks, and emerging patterns. The findings can aid researchers in gaining insight into the present landscape of the discipline and determining future research directions.
A Case of Metastatic Choriocarcinoma-Related Paraneoplastic Thyroid Storm
Authors: Gupta Nidhi , Graham Lauren , Carpenter Matthew , Gandhi Gunjan Y 
Published: March 2024

Abstract
Thyroid storm due to gestational trophoblastic disease (GTD) with metastatic choriocarcinoma is a rare but potentially life-threatening endocrine emergency. We report on a woman with molar pregnancy and metastatic choriocarcinoma who presented with thyroid storm (Burch-Wartofsky point scale of 45) a few weeks after the evacuation of GTD. She was initially managed with intravenous hydrocortisone, oral propylthiouracil (PTU), and esmolol infusion. After stabilization in the intensive care unit, 10 cycles of chemotherapy with etoposide, methotrexate, leucovorin, dactinomycin, and cyclophosphamide (EMA-CO) were initiated for stage 4 choriocarcinoma with brain and lung metastases. She underwent a hysterectomy soon after completing chemotherapy and received an additional 3 cycles of chemotherapy after the hysterectomy. As human chorionic gonadotropin (hCG) levels normalized, thyroid function reverted to normal as well. At the last follow-up, the patient was asymptomatic, euthyroid (without antithyroid medication), had a normal hCG titer of 1.7 mIU/mL (normal nonpregnant reference is &lt; 5 mIU/mL), and the lung and brain lesions had resolved entirely. Management of thyroid storm in the presence of untreated metastatic choriocarcinoma requires a high index of suspicion and a multidisciplinary team approach to prevent complications and improve survival.
Efficacy of Oral Cinacalcet in Non-PTH Nonmalignant Hypercalcemia from Excess 1,25-Dihydroxyvitamin D
Authors: Mohan Sneha , Sheehan Michael , Tebben Peter , Wermers Robert 
Published: March 2024

Abstract
Elevated 1,25-dihydroxyvitamin D (1,25(OH)2D) is a rare cause of non–parathyroid hormone (PTH)–mediated hypercalcemia seen in granulomatous disease, malignancy (most often lymphoma), or genetic mutations. Therapeutic options are limited. We report the case of a 67-year-old White man with nonmalignant, nongranulomatous, 1,25(OH)2D-mediated hypercalcemia treated successfully with cinacalcet. At presentation, he had hypercalcemia, hypercalciuria with recurrent nephrolithiasis, low PTH, elevated 1,25(OH)2D, and normal 25-hydroxyvitamin D. The 1,25(OH)2D levels were inappropriate in the setting of hypercalcemia with low PTH. Evaluations for sarcoidosis, tuberculosis, and malignancy were negative. Genetic testing showed biallelic variants in the CYP24A1 gene. Cinacalcet was trialed and showed normalization of calcium levels. On cinacalcet, biochemical indices showed a slight increase in 1,25(OH)2D and 24-hour urine calcium and mild decrease in PTH. He briefly experienced symptomatic hypocalcemia that resolved after reducing cinacalcet dose. Due to limited symptomatic benefit, he opted to stop cinacalcet. Additional follow-up showed intermittently elevated serum calcium levels after stopping cinacalcet, most recently 10.3 mg/dL. Cinacalcet may be a therapeutic option in nonmalignant, 1,25(OH)2D-mediated hypercalcemia. Further study is necessary to confirm efficacy, understand risks and benefits, and elucidate mechanism(s) of action.
Bone Density in Transgender Youth on Gender-Affirming Hormone Therapy
Authors: Roy Micaela K , Bothwell Samantha , Kelsey Megan M , Ma Nina S , Moreau Kerrie L , Nadeau Kristen J , Rothman Micol S , Nokoff Natalie J 
Published: March 2024

Abstract
Some transgender youth are treated with gonadotropin-releasing hormone agonists (GnRHa) followed by testosterone or estradiol, which may impact bone mineral density (BMD). This cross-sectional study of transgender youth (n = 56, aged 10.4-19.8 years, 53% assigned female at birth [AFAB]) utilized total body dual-energy x-ray absorptiometry to evaluate BMD Z-scores, and associations between GnRHa duration, body mass index (BMI), and BMD. Participants on GnRHa alone (n = 19, 14 assigned male at birth [AMAB], 5 AFAB) at the time of the study visit were 13.8 [12.8, 15.3] (median [IQR]) years old, had been on GnRHa for 10 [5.5, 19.5] months, and began GnRHa at age 12 [10.4, 12.6] years. Total body BMD Z-score for individuals on GnRHa monotherapy was −0.10 [−0.8, 0.4] (AFAB, female norms) and −0.65 [−1.4, 0.22] (AMAB, male norms). AFAB participants (n = 21) on testosterone were age 16.7 [15.9, 17.8] years, had been on testosterone for 11 [7.3, 14.5] months, and started testosterone at age 16 [14.8, 16.8] years; total body BMD Z-score −0.2 [−0.5, 0] (male norms) and 0.4 [−0.2, 0.7] (female norms). AMAB participants (n = 16) were age 16.2 [15.1, 17.4] years, had been on estradiol for 11 [5.6, 13.7] months, and started estradiol at age 16 [14.4, 16.7] years; total body BMD Z-score −0.4 [−1.1, 0.3] (male norms) and −0.2 [−0.7, 0.6] (female norms). BMD Z-score was negatively correlated with GnRHa duration (male norms: r = −0.5, P = .005; female norms: r = −0.4, P = .029) and positively correlated with BMI (male norms: r = 0.4, P = .003; female norms: r = 0.4, P = .004). In this cross-sectional cohort, total body BMD Z-scores were slightly below average, but lowest in the AMAB group on GnRHa monotherapy.
A Fracture Liaison Service to Address Vitamin D Deficiency for Patients Hospitalized for Osteoporotic Fracture
Authors: Sun Xiaoxu , Leder Benjamin Z , Bolster Marcy B , Ly Thuan V , Franco-Garcia Esteban , Pu Charles T , Fan WuQiang 
Published: March 2024

Abstract

Context
Addressing vitamin D deficiency (VDD) is important for fracture secondary prevention.

Objectives
To explore the function of a fracture liaison service (FLS) to address VDD.

Design, Setting and Patients
An observational study of patients admitted to the Massachusetts General Hospital with fractures between January 1, 2016, and October 31, 2023, cared for by the FLS.

Intervention
Ergocalciferol 50 000 international units (50ku-D2) oral daily for 3 to 7 days.

Main Outcomes Measures
VDD prevalence. Efficacy of inpatient daily 50ku-D2 in raising serum 25-hydroxyvitamin D (25OHD) levels.

Results
Of the 2951 consecutive patients, 724 (24.53%) had VDD (defined by 25OHD ≤ 19 ng/mL). Men (252/897, or 28.09%) were more likely than women (472/2054, or 22.98%) to have VDD (P = .003). VDD was seen in 41.79% (117/280), 24.41% (332/1360), and 20.98% (275/1311) of patients of aged ≤59, 60 to 79, and ≥80 years, respectively (P &lt; .00001). Of the 1303 patients with hip fractures, 327 (25.09%) had VDD, which was associated with a longer length of stay (8.37 ± 7.35 vs 7.23 ± 4.78 days, P = .009) and higher trend of 30-day-readmission rate (13.63% vs 18.35%, P = .037). In a cohort of 32 patients with complete data, each dose of 50ku-D2 increased serum 25OHD by 3.62 ± 2.35 ng/mL without affecting serum calcium or creatinine levels.

Conclusion
VDD was seen in nearly 25% of Massachusetts General Hospital FLS patients and more prevalent in male and younger patients. VDD was associated with longer length of stay and higher 30-day-readmission risk in patients with hip fracture. Daily 50ku-D2 appeared to be a practical way to quickly replete vitamin D in the inpatient setting.
Light-field photography using differential high-speed aperture coding
Authors: Huang Suyi , Yang Qin , Deng Zihao , Yao Manhong , Zhang Zibang , Liu Xiaoli , Li Jianping , Peng Junzheng , Zhong Jingang 
Published: March 2024

Programmable aperture light-field photography enables the acquisition of angular information without compromising spatial resolution. However, direct current (DC) background noise is unavoidable in images recorded by programmable aperture light-field photography, leading to reducing the contrast of reconstructed images. In addition, it requires sacrificing temporal resolution to obtain angular information, making it a challenge to capture dynamic scenes. In this paper, we propose programmable aperture light-field photography using differential high-speed aperture coding. This method effectively reduces DC noise and produces high-contrast refocused images. Furthermore, we build a light-field camera based on a 1250 Hz spatial light modulator and a 1250 fps high-speed camera, achieving dynamic light-field photography at 1110(H)×800(V) resolution and 24 fps. Our results demonstrate significant improvements in image contrast and exhibit considerable promise for diverse applications.
Influence of Sperm Number and Antioxidant Melatonin in Extender on the Quality of Post-Thawing Sheep Spermatozoa
Authors: Putri Frilianty , Karja Ni Wayan Kurniani , Setiadi Mohamad Agus , Kaiin Ekayanti Muyawati 
Published: March 2024

This study aimed to examine the effect of spermatozoa concentration and the effectiveness of melatonin supplementation in diluent on the quality of post-thawing semen.Â Ejaculated semen was collected using the artificial vaginal method (MVB).Â The study was carried out in two stages, firstly semen was frozen in andromed diluent with different concentrations in one straw (50, 100, 200 million per straw), and the second was frozen semen in diluent supplemented with melatonin with different doses (0, 0,5, 1, 0 and 1.5 mM melatonin).Â Parameters observed were the movement of spermatozoa using Computer Assisted Sperm Analysis, membrane integrity, and acrosome integrity.Â Data were analyzed using ANOVA and further tested using Duncan's test.Â The results showed no significant difference in the quality and movement pattern of sheep semen when frozen at concentrations of 50, 100, or 200 million per straw (P 0.05).Â Furthermore, adding melatonin to the diluent in this study affected spermatozoa's total motility and progressive motility at a concentration of 1.0 mM (P0.05) but did not significantly affect the percentage of spermatozoa motility pattern characteristics.Â From the results, it can be concluded that the concentration of per straw spermatozoa does not affect the quality of sheep semen, and melatonin supplementation in diluent can reduce the effects of the frozen-thawed process on motility, acrosome cap, and plasma membrane integrity in sheep semen.Â Melatonin supplementation with a concentration of 1 mM in the extender was the highest quality concentration in this study.
Quality of Physical and Sensory of Super-native Chicken Breast Marinated with Herbs and Spices with Different Levels of Marination Concentration
Authors: Oktafa Huda , Prayitno Agus Hadi , Handayani Hatmiyarni Tri 
Published: March 2024

Super-native chickens result from crosses between male native chickens and laying hens.Â The diversification of processed products from super-native chicken can be developed into ready-to-cook products by marinating them with local herbs and spices. Â This research was conducted on the effect of marination concentrations of different local herbs and spices on the hedonic quality of super-native chicken breast.Â The research material consisted of super-native chicken breast, chili powder, sugar, salt, pepper, garlic, cinnamon, palm oil, lime juice, monosodium glutamate, and ginger.Â The treatment levels of marination concentration were 10, 20, and 30% of the weight of the meat.Â Physical quality parameters tested were color, pH value, moisture, cooking loss, water-holding capacity, yield, and hardness.Â Each treatment consisted of 3 replications.Â In addition, sensory quality was tested by a hedonic quality test by 40 untrained panelists.Â The hedonic quality parameters tested were color, flavor, taste, texture, tenderness, and juiciness.Â The data from the physical quality test were analyzed by analysis of variance.Â The hedonic quality test results were analyzed by non-parametric analysis through the Kruskal-Wallis hedonic test.Â If there was a significant difference (P0.05), the test was continued with Duncan's New Multiple Range Test. Â The results showed that the concentration level of different local herbs and spices marination could affect the physical quality (redness, pH value, and moisture) and sensory (color and texture) of super-native chicken breast.Â The marination concentration of local herbs and spices at 20% level was the best treatment based on hedonic quality with a smooth meat texture and a score of 3.98.
Construção e Interpretação de Tabelas e Gráficos Estatísticos: um olhar para a atitude de professores em formação
Authors: Castro Eliziane Rocha , Barreto Marcilia Chagas , Nascimento Francisco Jeovane do , Sousa Gabriel Linhares de 
Published: March 2024

Este estudo analisou a atitude de professoras em formação em relação à construção e interpretação de tabelas e gráficos estatísticos após a conclusão de uma atividade que demandou essas habilidades. Sete professoras que atuavam no 5° ano do ensino fundamental em Raposa/MA participaram da pesquisa. Optou-se por uma abordagem qualitativa empregando-se a Pesquisa-ação como método e conduzindo-se a análise documental, que envolveu a análise de conteúdo com o auxílio do software NVIVO. Os resultados revelaram que as professoras reconheceram a utilidade de tabelas e gráficos estatísticos, demonstraram baixa confiança em seu conhecimento intelectual, e experimentaram sentimentos negativos e ambivalentes. Também demonstraram disposição para enfrentar desafios, especialmente na interpretação de gráficos pictóricos, buscando autonomia na construção e interpretação desses gráficos. Essas atitudes refletem conscientização da complexidade das habilidades estatísticas e busca por aprimoramento sendo cruciais para promover uma prática educacional eficaz e desenvolver o letramento estatístico dos professores em formação.
Weak Procedural Constitutionalism. The Judicial Process as Legitimacy of Judicial Review
Authors: Jiménez Ramírez Milton César 
Published: March 2024

Abstract
This paper proposes a synthesis that renders democratic principles compatible with the preservation of judicial control of constitutionality. This has been mediated by what I have called ‘weak procedural constitutionalism’, a methodology through which constitutional conflicts are debated in the legislature and among the citizenry as the real holders of the final say in society, with judicial activity focused on an intermediate say and the promotion of subsequent social deliberation. The judicial process thus becomes a public dialogical procedure susceptible to intervention by citizens and capable of generating public information processes facilitating accountability. The constant quest for instrumentalities that increase the democratic legitimacy of constitutional courts is an existential necessity. Only through real opportunities for dialogue and citizen participation in the decision-making process can judicial review be made democratically palatable. This requires that both judges and legislators play an intermediate rather than primary role, one in which democratically elected legislatures enjoy a high degree of legitimacy in adopting decisions, in juxtaposition with the derivative legitimacy appurtenant to constitutional courts. The premise for the exercise of what I denominate as weak constitutionality control, or the hypothesis for a weak procedural constitutionalism, is a possible synthesis of the tension between constitutionalism and democracy, with emphasis on the Colombian case.