Identification and recombinant expression of a cutinase from
Papiliotrema laurentii
that hydrolyzes natural and synthetic polyesters
Authors: Roman Victor A. , Crable Bryan R. , Wagner Dominique N. , Gryganskyi Andrii , Zelik Stephen , Cummings Logan , Hung Chia S. , Nadeau Lloyd J. , Schratz Lucas , Haridas Sajeet , Pangilinan Jasmyn , Lipzen Anna , Na Hyunsoo , Yan Mi , Ng Vivian , Grigoriev Igor V. , Barlow Daniel , Biffinger Justin , Kelley-Loughnane Nancy , Crookes-Goodson Wendy J. , Stamps Blake , Varaljay Vanessa A. 
Published: April 2024

ABSTRACT

Given the multitude of extracellular enzymes at their disposal, many of which are designed to degrade nature’s polymers (lignin, cutin, cellulose, etc.), fungi are adept at targeting synthetic polyesters with similar chemical composition. Microbial-influenced deterioration of xenobiotic polymeric surfaces is an area of interest for material scientists as these are important for the conservation of the underlying structural materials. Here, we describe the isolation and characterization of the
Papiliotrema laurentii
5307AH (
P. laurentii
) cutinase, Plcut1.
P. laurentii
is basidiomycete yeast with the ability to disperse Impranil-DLN (Impranil), a colloidal polyester polyurethane, in agar plates. To test whether the fungal factor involved in this clearing was a secreted enzyme, we screened the ability of
P. laurentii
culture supernatants to disperse Impranil. Using size exclusion chromatography (SEC), we isolated fractions that contained Impranil-clearing activity. These fractions harbored a single ~22 kD band, which was excised and subjected to peptide sequencing. Homology searches using the peptide sequences identified, revealed that the protein Papla1 543643 (Plcut1) displays similarities to serine esterase and cutinase family of proteins. Biochemical assays using recombinant Plcut1 confirmed that this enzyme has the capability to hydrolyze Impranil, soluble esterase substrates, and apple cutin. Finally, we confirmed the presence of the Plcut1 in culture supernatants using a custom antibody that specifically recognizes this protein. The work shown here supports a major role for the Plcut1 in the fungal degradation of natural polyesters and xenobiotic polymer surfaces.

IMPORTANCE

Fungi play a vital role in the execution of a broad range of biological processes that drive ecosystem function through production of a diverse arsenal of enzymes. However, the universal reactivity of these enzymes is a current problem for the built environment and the undesired degradation of polymeric materials in protective coatings. Here, we report the identification and characterization of a hydrolase from
Papiliotrema laurentii
5307AH, an aircraft-derived fungal isolate found colonizing a biodeteriorated polymer-coated surface. We show that
P. laurentii
secretes a cutinase capable of hydrolyzing soluble esters as well as ester-based compounds forming solid surface coatings. These findings indicate that this fungus plays a significant role in biodeterioration through the production of a cutinase adept at degrading ester-based polymers, some of which form the backbone of protective surface coatings. The work shown here provides insights into the mechanisms employed by fungi to degrade xenobiotic polymers.