bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling of Polar Ocean Tides at the Last Glacial Maximum: Amplification, Sensitivity, and Climatological Implications
Authors: Griffiths Stephen D. , Peltier W. Richard 
Published: December 2008

AbstractDiurnal and semidiurnal ocean tides are calculated for both the present day and the Last Glacial Maximum. A numerical model with complete global coverage and enhanced resolution at high latitudes is used including the physics of self-attraction and loading and internal tide drag. Modeled present-day tidal amplitudes are overestimated at the standard resolution, but the error decreases as the resolution increases. It is argued that such results, which can be improved in the future using higher-resolution simulations, are preferable to those obtained by artificial enhancement of dissipative processes. For simulations at the Last Glacial Maximum a new version of the ICE-5G topographic reconstruction is used along with density stratification determined from coupled atmosphere–ocean climate simulations. The model predicts a significant amplification of tides around the Arctic and Antarctic coastlines, and these changes are interpreted in terms of Kelvin wave dynamics with the aid of an exact analytical solution for propagation around a polar continent or basin. These polar tides are shown to be highly sensitive to the assumed location of the grounding lines of coastal ice sheets, and the way in which this may contribute to an interaction between tides and climate change is discussed. Globally, the picture is one of energized semidiurnal tides at the Last Glacial Maximum, with an increase in tidal dissipation from present-day values, the dominant energy sink being the conversion to internal waves.
Quantifying the Imprint of a Severe Hector Thunderstorm during ACTIVE/SCOUT-O3 onto the Water Content in the Upper Troposphere/Lower Stratosphere
Authors: Chemel Charles , Russo Maria R. , Pyle John A. , Sokhi Ranjeet S. , Schiller Cornelius 
Published: December 2008

Abstract
The development of a severe Hector thunderstorm that formed over the Tiwi Islands, north of Australia, during the Aerosol and Chemical Transport in Tropical Convection/Stratospheric-Climate Links with Emphasis on the Upper Troposphere and Lower Stratosphere (ACTIVE/SCOUT-O3) field campaign in late 2005, is simulated by the Advanced Research Weather Research and Forecasting (ARW) model and the Met Office Unified Model (UM). The general aim of this paper is to investigate the role of isolated deep convection over the tropics in regulating the water content in the upper troposphere/lower stratosphere (UT/LS). Using a horizontal resolution as fine as 1 km, the numerical simulations reproduce the timing, structure, and strength of Hector fairly well when compared with field campaign observations. The sensitivity of results from ARW to horizontal resolution is investigated by running the model in a large-eddy simulation mode with a horizontal resolution of 250 m. While refining the horizontal resolution to 250 m leads to a better representation of convection with respect to rainfall, the characteristics of the Hector thunderstorm are basically similar in space and time to those obtained in the 1-km-horizontal-resolution simulations. Several overshooting updrafts penetrating the tropopause are produced in the simulations during the mature stage of Hector. The penetration of rising towering cumulus clouds into the LS maintains the entrainment of air at the interface between the UT and the LS. Vertical exchanges resulting from this entrainment process have a significant impact on the redistribution of atmospheric constituents within the UT/LS region at the scale of the islands. In particular, a large amount of water is injected in the LS. The fate of the ice particles as Hector develops drives the water vapor mixing ratio to saturation by sublimation of the injected ice particles, moistening the air in the LS. The moistening was found to be fairly significant above 380 K and averaged about 0.06 ppmv in the range 380–420 K for ARW. As for UM, the moistening was found to be much larger (about 2.24 ppmv in the range of 380–420 K) than for ARW. This result confirms that convective transport can play an important role in regulating the water vapor mixing ratio in the LS.
Predictive Factors of Subtle Residual Shoulder Symptoms after Transtendinous Arthroscopic Cuff Repair
Authors: Castagna Alessandro , Delle Rose Giacomo , Conti Marco , Snyder Stephen J. , Borroni Mario , Garofalo Raffaele 
Published: December 2008

Background Transtendinous repair is a well-known technique for the arthroscopic management of partial rotator cuff tear. However, there are not a lot of clinical follow-up studies in the literature reporting data on this approach, and, moreover, potential factors responsible to influence the outcomes have not been investigated. Purpose To evaluate clinical outcomes after arthroscopic transtendinous repair and to identify predictive factors of residual shoulder symptoms. Study Design Case series; Level of evidence, 4. Methods Fifty-four patients with a mean age of 56.7 ± 8.8 years (range, 31-71 years) who had undergone an arthroscopic transtendon repair for a painful articular-sided rotator cuff tear with a minimum of 2 years of follow-up were contacted. Clinical outcomes using a patient-based questionnaire, the Constant score, University of California at Los Angeles score, Simple Shoulder Test, and visual analog scale were evaluated. The influence of patient age, presence or absence of a trauma responsible for the cuff tear, presence of associated shoulder lesions, millimeters of exposed footprint, and millimeters of torn tendon retraction on the outcomes were assessed. Results The mean cuff tear exposure footprint was 5.2 mm, and the mean retraction of the torn part of the tendon was 8 mm. Only one patient reported dissatisfaction with surgery because of persistence of pain during overhead activities. After arthroscopic repair, University of California at Los Angeles, Constant, and Simple Shoulder Test scores were significantly improved from 14.1, 45.3, and 9.8 to 32.9, 90.6, and 0.8, respectively (P < .001). Twenty-two patients (41%) reported occasional shoulder discomfort at the extremes of range of motion (particularly at extremes of abduction and internal rotation) occurring during some daily living and sports activities. The best multivariate model showed that residual shoulder discomfort is strongly linked with a partial thickness supraspinatus tendon avulsion-type articular-sided rotator cuff lesion consisting of a large tendon retraction and/or a relatively small exposure footprint area in an older patient in the absence of a specific trauma (P < .001). Conclusion Arthroscopic transtendon partial articular supraspinatus tendon avulsion-type rotator cuff repair was a reliable procedure that resulted in a good outcome in terms of pain relief and shoulder scores in 98% of the 54 patients. Better results could be expected in patients with less tendon retraction, a larger footprint exposure, of younger age, and with a clinical history of trauma.
A New Peroneal Tendon Rerouting Method to Treat Recurrent Dislocation of Peroneal Tendons
Authors: Wang Chih-Chien , Wang Shyu-Jye , Lien Shiu-Bii , Lin Liou-Chyr 
Published: December 2008

Background The surgical treatment methods for recurrent dislocation of peroneal tendons are controversial. A simpler and more effective treatment method is valuable for these patients. Hypothesis A new rerouting operation designed by the authors will have satisfactory results and avoid disadvantages of the old rerouting methods. Study Design Case series; Level of evidence, 4. Methods Seventeen consecutive male patients with unilateral recurrent peroneal tendon dislocations were treated by transposition of the calcaneofibular ligament from the tubercle of calcaneofibular ligament with a 1 × 1 × 1 cm3 bone block and elevation of this tubercle with another 1 × 1 × 1 cm3 calcaneal bone block, which were fixed by a 3.5-mm cancellous screw with a washer. All patients received clinical and radiographic follow-up for at least 2 years. The preoperative and postoperative American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot scales were used for functional results assessment. Results All bone transposition sites healed radiographically at 6 weeks after surgery. Four patients had transient numbness over the lateral aspect of the injured foot, and 3 patients had swelling and pain involving the operative sites. All complications resolved by 3 to 5 months after the operation. No recurrent dislocation of the peroneal tendons was noted. The mean AOFAS ankle-hindfoot scale improved significantly, from 73.4 ± 5.5 preoperatively to 100 at 2- to 5-year follow-up (P <. 001). Normal ankle stability and no tightening of the lateral side of the injured ankles in the inversion position were noted. Conclusion This method is a simple, reliable, and reproducible operation to treat recurrent dislocation of the peroneal tendons. It allows early return to daily, working, and sports activities with satisfactory results.
Effect of Bone Morphogenetic Protein-12 Gene Transfer on Posterior Cruciate Ligament Healing in a Rabbit Model
Authors: Ma Yong , Zhang Xin , Wang Jian , Liu Ping , Zhao Liheng , Zhou Chunyan , Ao Yingfang 
Published: December 2008

Background The posterior cruciate ligament heals to some extent after injury. However, results after conservative treatment may diminish with long-term follow-up. Bone morphogenetic protein-12 can induce formation of ligament tissues. Hypothesis Bone morphogenetic protein-12 gene transfer can improve the histologic and biomechanical properties of healing posterior cruciate ligaments. Study Design Controlled laboratory study. Methods Bilateral posterior cruciate ligaments of 32 rabbits were injured. The cut ends in 1 limb received an injection containing 3 × 107 pfu recombinant bone morphogenetic protein-12 adenovirus, and the posterior cruciate ligament in the contralateral limb served as an untreated control. Eight rabbits were sacrificed at each time point of 3, 6, 12, and 26 weeks after the operation. In addition, 6 rabbits receiving a sham operation were used to obtain normal control data. The posterior cruciate ligament specimens were evaluated biomechanically and histologically. Results The repair tissue of the treatment group at 26 weeks was similar to the normal posterior cruciate ligament in collagen arrangement, collagen formation, and mechanical properties. At weeks 6, 12, and 26, the ultimate load, stiffness, and energy absorbed at failure of the treatment group were significantly greater than those of the untreated group. Conclusion Adenovirus-mediated bone morphogenetic protein-12 gene transfer in a partial posterior cruciate ligament laceration rabbit model resulted in an obvious improvement of histologic properties, tensile strength, and stiffness of the repaired ligaments, indicating improved posterior cruciate ligament healing. Clinical Relevance Bone morphogenetic protein-12 gene transfer is a potential future strategy to improve the repair of injured posterior cruciate ligaments.
Minimum 10-Year Results after Anterior Cruciate Ligament Reconstruction
Authors: Shelbourne K.Donald , Gray Tinker 
Published: December 2008

Background Few long-term studies exist that evaluate how the loss of normal knee range of motion affects results after anterior cruciate ligament reconstruction. Hypothesis Patients with normal knee motion will have higher subjective scores than patients with less than normal motion. Study Design Cohort study; Level of evidence, 2. Methods Patients were prospectively evaluated at > 10 years after anterior cruciate ligament reconstruction according to International Knee Documentation Committee criteria. Normal knee motion was within 2° of extension (including hyperextension) and 5° of flexion compared with the uninvolved knee. Regression analysis was performed to determine what factors affected subjective scores. Results Objective follow-up was obtained on 502 patients at a mean of 14.1 years postoperatively. Regression analysis showed that the most statistically significant factor related to lower subjective scores was lack of normal knee extension; loss of normal flexion was also significant. Patients who had meniscectomy or articular cartilage damage had statistically significantly lower subjective scores if they also had less than normal motion. Ninety-eight percent of patients with intact menisci, normal articular cartilage, and normal knee motion had normal radiographs; 29% of patients with normal motion had less than normal radiographs versus 71% of patients who had less than normal motion. The overall International Knee Documentation Committee objective grade was normal in 48%, nearly normal in 42%, abnormal in 9%, and severely abnormal in 0.5%. Conclusion The loss of 3° to 5° of knee extension, to include loss of hyperextension, adversely affected the subjective and objective results after surgery, especially when coupled with meniscectomy and articular cartilage damage.
T-cell receptor– and CD28-induced Vav1 activity is required for the accumulation of primed T cells into antigenic tissue
Authors: David Rachel , Ma Liang , Ivetic Aleksandar , Takesono Aya , Ridley Anne J. , Chai Jian-Guo , Tybulewicz Victor L. , Marelli-Berg Federica M. 
Published: December 2008

Abstract
Localization of primed T cells to antigenic tissue is essential for the development of effective immunity. Together with tissue-selective homing molecules, T-cell receptor (TCR)– and CD28-mediated signals have been shown to promote transendothelial migration of specific T cells into nonlymphoid antigen-rich tissue. However, the cellular and molecular requirements for T-cell accumulation to target tissue following their recruitment are largely undefined. The guanine nucleotide exchange factor (GEF) Vav1 has an integral role in coupling TCR and CD28 to signaling pathways that regulate T-cell activation and migration. Here, we have investigated the contribution of TCR- and CD28-induced Vav1 activity to the trafficking and localization of primed HY-specific CD4+ T cells to antigenic sites. Severe migratory defects displayed by Vav1−/− T cells in vitro were fully compensated by a combination of shear flow and chemokines, leading to normal recruitment of Vav1−/− T cells in vivo. In contrast, Vav1−/− T-cell retention into antigen-rich tissue was severely impaired, reflecting T cells' inability to engage in sustained TCR- and CD28-mediated interactions with tissue-resident antigen-presenting cells (APCs). This novel function of APC-induced, and TCR- and CD28-mediated Vav1 activity in the regulation of effector T-cell immunity highlights its potential as a therapeutic target in T cell–mediated tissue damage.
Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes.
Authors: Luo Xin M. , Maarschalk Emily , O'Connell Ryan M. , Wang Pin , Yang Lili , Baltimore David 
Published: December 2008

AbstractBroadly neutralizing anti-HIV antibodies are rare and have proved hard to elicit with any immunogen. We have tested in vitro the notion that such antibodies or other antiviral proteins could be made by lentivirus-mediated gene transfer into human hematopoietic stem/progenitor cells (HSPCs), followed by differentiation of the transduced cells into B cells, the most potent antibody-producing cells. To do this, we have developed a highly efficient system for in vitro maturation of secreting B lymphocytes and plasma cells from CD34+ HSPCs. It is a 3-stage, in vitro culture system that supports normal human B-lineage development from HSPCs to antibody-secreting plasmablasts (∼36%) and plasma cells (∼20%). By transducing human cord blood CD34+ cells with lentiviral vectors encoding a secretory monoclonal anti-HIV antibody, b12 (IgG1), we were able to program human B cells to produce in vitro up to 1.5 μg/mL of this broadly neutralizing antibody. Our results suggest that an HIV vaccine might be delivered by autologous transplantation of in vitro–programmed HSPCs, which would develop into antibody-secreting B cells in vivo and provide a continuous supply of anti-HIV neutralizing antibodies.
Mind the gap between policy imperatives and service provision: a qualitative study of the process of respiratory service development in England and Wales
Authors: Hamilton Sonya , Huby Guro , Tierney Alison , Powell Alison , Kielmann Tara , Sheikh Aziz , Pinnock Hilary 
Published: December 2008

Abstract

Background
Healthcare systems globally are reconfiguring to address the needs of people with long-term conditions such as respiratory disease. Primary Care Organisations (PCOs) in England and Wales are charged with the task of developing cost-effective patient-centred local models of care. We aimed to investigate how PCOs in England and Wales are reconfiguring their workforce to develop respiratory services, and the background factors influencing service redesign.

Methods
Semi-structured qualitative telephone interviews with the person(s) responsible for driving respiratory service reconfiguration in a purposive sample of 30 PCOs. Interviews were recorded, transcribed, coded and thematically analysed.

Results
We interviewed representatives of 30 PCOs with diverse demographic profiles planning a range of models of care. Although the primary driver was consistently identified as the need to respond to a central policy to shift the delivery of care for people with long-term conditions into the community whilst achieving financial balance, the design and implementation of services were subject to a broad range of local, and at times serendipitous, influences. The focus was almost exclusively on the complex needs of patients at the top of the long-term conditions (LTC) pyramid, with the aim of reducing admissions. Whilst some PCOs seemed able to develop innovative care despite uncertainty and financial restrictions, most highlighted many barriers to progress, describing initiatives suddenly shelved for lack of money, progress impeded by reluctant clinicians, plans thwarted by conflicting policies and a PCO workforce demoralised by job insecurity.

Conclusion
For many of our interviewees there was a large gap between central policy rhetoric driving workforce change, and the practical reality of implementing change within PCOs when faced with the challenges of limited resources, diverse professional attitudes and an uncertain organisational context. Research should concentrate on understanding these complex dynamics in order to inform the policymakers, commissioners, health service managers and professionals.
Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGFβ-1 in cultured rat nucleus pulposus cells
Authors: Nakai Tomoko , Mochida Joji , Sakai Daisuke 
Published: December 2008

Abstract
Introduction
Although transforming growth factor β1 (TGFβ1) is known to be a potent inhibitor of proliferation in most cell types, it accelerates proliferation in certain mesenchymal cells, such as articular chondrocytes and nucleus pulposus cells. The low ability for self-renewal of nucleus pulposus cells is one obstacle in developing new therapeutic options for intervertebral disc diseases, and utilizing cytokines is one of the strategies to regulate nucleus pulposus cell proliferation. However, the precise cell cycle progression and molecular mechanisms by which TGFβ1 stimulates cell growth remain unclear. The aim of this study was to elucidate a mechanism that enables cell proliferation with TGFβ1 stimulation.

Methods
We tested cultured rat nucleus pulposus cells for proliferation and cell cycle distribution under exogenous TGFβ1 stimulation with and without putative pharmaceutical inhibitors. To understand the molecular mechanism, we evaluated the expression levels of key regulatory G1 phase proteins, c-Myc and the cyclin-dependent kinase inhibitors.

Results
We found that TGFβ1 promoted proliferation and cell cycle progression while reducing expression of the cyclin-dependent kinase inhibitors p21 and p27, which are downregulators of the cell cycle. Robust c-Myc expression for 2 h and immediate phosphorylation of extra cellular signal regulated kinase (ERK1/2) were detected in cultures when TGFβ1 was added. However, pretreatment with 10058-F4 (an inhibitor of c-Myc transcriptional activity) or PD98059 (an inhibitor of ERK1/2) suppressed c-Myc expression and ERK1/2 phosphorylation, and inhibited cell cycle promotion by TGFβ1.

Conclusions
Our experimental results indicate that TGFβ1 promotes cell proliferation and cell cycle progression in rat nucleus pulposus cells and that c-Myc and phosphorylated ERK1/2 play important roles in this mechanism. While the difference between rat and human disc tissues requires future studies using different species, investigation of distinct response in the rat model provides fundamental information to elucidate a specific regulatory pathway of TGFβ1.
IFN-β improves BCG immunogenicity by acting on DC maturation
Authors: Giacomini Elena , Remoli Maria Elena , Gafa Valérie , Pardini Manuela , Fattorini Lanfranco , Coccia Eliana M 
Published: December 2008

AbstractGiven the variable protective efficacy provided by Mycobacterium bovis bacillus Calmette-Guérin (BCG), there is an urgent need to develop new vaccines against tuberculosis. As dendritic cells (DC) play a critical role in initiating and regulating a protective T cell response against the pathogens, the comprehension of mycobacterium-induced modulation of DC functions is critical to pinpoint new, immunological strategies. To this end, a comparative analysis of the effect induced by BCG and Mycobacterium tuberculosis (Mtb) infection on the DC immunophenotype indicated that BCG is less efficient in inducing DC maturation than Mtb. In addition, BCG-infected DC poorly expressed IFN-β and displayed a reduced production of IL-12 as compared with Mtb-stimulated cells. The impaired expression of IL-12p35 and IFN-β is likely a result of the inability of BCG to induce the activation of the IFN regulatory factor-3. Taking into account these data, we sought to investigate whether the exogenous addition of IFN-β, a cytokine that exerts important effects on the immune system, could enhance the Th1-polarizing capacity of BCG-infected DC. Interestingly, when DC infected by BCG were pretreated in vitro with IFN-β, they displayed a fully mature phenotype and released a significant amount of bioactive IL-12p70, which resulted in an enhanced Th1 response. This study demonstrates that IFN-β potentiates DC immunological functions following BCG infection, thus suggesting IFN-β as a possible candidate as vaccine adjuvant.