bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neurochemical markers of brain damage in cerebrospinal fluid during induction treatment of acute lymphoblastic leukemia in children
Authors: Österlundh Gustaf , Kjellmer Ingemar , Lannering Birgitta , Rosengren Lars , Nilsson Ulf A. , Márky Ildikó 
Published: October 2007

AbstractBackgroundCentral nervous system (CNS) irradiation has been replaced by systemic high‐dose methotrexate (MTX) and intrathecal MTX in acute lymphoblastic leukemia treatment due to the risk of late effects. However, treatment without CNS irradiation might also cause brain damage.ProcedureCerebrospinal fluid (CSF) was analyzed in 121 patients in an attempt to detect CNS injury. Seventy‐three samples were analyzed for neuron‐specific enolase (NSE), 108 for glial fibrillary acidic protein (GFAp), 110 for neurofilament protein light chain (NFp), and 70 for ascorbyl radical (AsR). Samples were taken at day 0, 8, 15, and 29 during induction treatment, including intrathecal MTX. Levels at days 8, 15, and 29 were compared with the levels before treatment.ResultsNSE levels were 9.0 (±3.5) µg/L (mean (±SD)) at day 0, 15.0 (±5.3) at day 8 (P < 0.001), 13.6 (±4.7) at day 15 (P < 0.001) and 11.1 (±4.3) at day 29 (P < 0.001). GFAp were 177 (±98) ng/L at day 0, 206 (±101) at day 8 (P < 0.001), 200 (±106) at day 15 (n.s.) and 228 (±137) at day 29 (P < 0.001). NFp were below the detection limit 125 ng/L at day 0 in all 110 CSF samples analyzed, and increased significantly above the detection limit in 6/77 samples at day 8, in 11/84 at day 15 and in 22/91 at day 29. The AsR content did not change significantly.ConclusionsLevels of NSE, GFAp, and NFp increased in CSF, which can be interpreted as early signs of brain damage. AsR levels do not show any convincing signs of oxidative stress. Pediatr Blood Cancer 2008;50:793–798. © 2007 Wiley‐Liss, Inc.
Brain‐sparing radiotherapy for neuroblastoma skull metastases
Authors: Wolden Suzanne L. , Barker Christopher A. , Kushner Brian H. , Bodduluri Haritha , Della‐Biancia Cesar , Kramer Kim , Modak Shakeel , Cheung Nai‐Kong V. 
Published: October 2007

AbstractBackgroundNeuroblastoma (NB) frequently metastasizes to the skull, often diffusely involving the calvarium and skull base. Radiotherapy may enhance local control; however, irradiating the brain is undesirable in young patients. The purpose of this study was to describe the technique, outcome and toxicities in patients with high risk NB metastatic to the skull treated with brain‐sparing skull radiotherapy (BSRT).ProcedureBetween 1999 and 2007, 31 patients with INSS stage four high risk NB, aged 2–32 years (median 6 years), underwent multimodality therapy, including radiotherapy to the whole skull using a brain‐sparing technique never previously described in this population. Dosimetric analyses were performed to compare the BSRT technique to a whole brain radiotherapy (WBRT) technique. Patients were either treated to consolidate upfront induction therapy (n = 22) or to palliate relapsed disease (n = 9).ResultsThirty of 31 patients (97%) completed the full course of BSRT. Median follow‐up was 19 months (range 1–83 months). Radiographic response to therapy was noted in 89% of patients. The actuarial rate of disease control in the skull was 89% and 60% 1 year after starting BSRT in patients treated in consolidation and for palliation, respectively. BSRT delivered half of the mean radiation dose to the brain when dosimetrically compared to whole brain radiotherapy. Few patients experienced significant toxicity.ConclusionsBSRT in NB patients with diffuse skull metastases offers dosimetric advantages over WBRT and results in good local control when used in the consolidative setting. The technique is well tolerated and while toxicity appears acceptable, longer follow‐up is necessary. Pediatr Blood Cancer 2008;50:1163–1168. © 2007 Wiley‐Liss, Inc.
UICC‐2002 TNM classification is not suitable for differentiated thyroid cancer in children and adolescents
Authors: Oommen Prasad T. , Romahn Annika , Linden Tobias , Frühwald Michael C. , Bucsky Peter 
Published: October 2007

AbstractBackgroundRecently the UICC‐TNM classification for differentiated thyroid cancer (DTC) was changed neglecting the special circumstances for children affected by the disease. While the 1997 TNM classification grouped tumours ≤1 cm as T1, the 2002 system changed this to a margin of ≤2 cm. The consequences of this change were evaluated by analysing patients enrolled in the multicentre interdisciplinary therapy study of the German Society of Paediatric Oncology and Haematology (GPOH) on malignant endocrine tumours in children and adolescents, GPOH‐MET 97.ProcedureBetween 1998 and 2005, 82 patients with histologically proven DTC entered the study. Patients classified according to UICC‐TNM classification 1997 were reclassified according to the new classification (2002/2003) and vice versa by cross checking with original pathologist's reports.ResultsTwenty males and 62 females at a mean age of 12.5 years were evaluated. We observed a definite shift from patients formerly classified as T2 (1–4 cm) to category T1 (≤2 cm) according to the 2002 TNM classification. Among these patients a threefold increase of lymph node involvement and/or distant metastases could be demonstrated.ConclusionsThe 2002 UICC‐classification may have a disadvantage for children with tumours measuring between 1 and 2 cm, as those are now classified as T1. A high rate of lymph node involvement in this group reflects the risk of under‐diagnosis and ‐treatment of this group. The current TNM classification for DTC in children should be changed taking the physiological and anatomical differences between children and adults into consideration. Pediatr Blood Cancer 2008;50:1159–1162. © 2007 Wiley‐Liss, Inc.
Early relapse after rituximab chemoimmunotherapy
Authors: Kiss Flora , Buslig Julia , Szegedi Istvan , Scholtz Beata , Kappelmayer Janos , Kiss Csongor 
Published: October 2007

AbstractIn relapsed/refractory childhood acute lymphoblastic leukemia (ALL) of the B‐cell lineage rituximab, a monoclonal anti‐CD20 antibody was used successfully in some cases. We report on a 15‐year‐old female with relapsed CD20‐positive B‐cell progenitor ALL treated with rituximab because of positive minimal residual disease signals after chemotherapy, as checked by flow cytometry and real time quantitative‐PCR. Rituximab eliminated the CD20‐positive subpopulation, but not the more immature leukemic cells. The patient died with fulminant aspergillosis before hematopoietic stem cell transplantation could be performed. Pediatr Blood Cancer 2008;50:372–375. © 2007 Wiley‐Liss, Inc.
Transient sialoadenitis: A complication of 131I‐metaiodobenzylguanidine therapy
Authors: Modak Shakeel , Pandit‐Taskar Neeta , Kushner Brian H. , Kramer Kim , Smith‐Jones Peter , Larson Steven , Cheung Nai‐Kong V. 
Published: October 2007

AbstractRadioiodinated metaiodobenzylguanidine [131I‐MIBG] is commonly used to treat resistant neuroblastoma or metastatic pheochromocytoma [MP] with little non‐hematopoietic toxicity. We describe here transient sialoadenitis, a previously unreported complication. Ten patients [9 neuroblastoma and 1 MP] received 12–18 mCi/kg of 131I‐MIBG. Five patients had bilateral parotid swelling, two with associated buccal discomfort within 24 hr of injection which subsided within 48 hr. Grade 3 or 4 serum amylase elevation was documented in 8/8 patients tested [median 1,336; range: 576–8,830 U/L] which normalized [25–125 U/L] within 4–14 [median 5.5] days. Serum lipase remained normal. Patients did not develop subsequent dry mouth or dysphagia. Pediatr Blood Cancer 2008;50:1271–1273. © 2007 Wiley‐Liss, Inc.
Cultural adaptation, translation and validation of a functional outcome questionnaire (TESS) to Portuguese with application to patients with lower extremity osteosarcoma
Authors: Saraiva Daniela , de Camargo Beatriz , Davis Aileen M. 
Published: October 2007

AbstractBackgroundEvaluation of physical functioning is an important tool for planning rehabilitation. Instruments need to be culturally adapted for use in non‐English speaking countries. The aim of this study was to culturally adapt, including translation and preliminary validation, the Toronto extremity salvage score (TESS) for Brazil, in a sample of adolescents and young adults treated for lower extremity osteosarcoma.ProcedureThe process included two independent forward translations of TESS questionnaire, consensus between translators on a forward translation, back‐translation by two independent translators, and a review of the back‐translations. Internal consistency of the TESS and known groups validity were also evaluated.ResultsInternal consistency for the 30 item TESS was high (coefficient alpha = 0.87). TESS score ranges from 0 to 100. Forty‐eight patients completed the questionnaire and scores ranged from 56 to 100 (mean score: 89.6). Patients receiving no pain medications scored higher on the TESS than those who were receiving pain medication (P = 0.014), and patients using walking aids had slightly higher but not statistically different scores. Those who were treated with amputation had higher scores than those who were treated with limb salvage procedures (P = 0.003).ConclusionPreliminary evidence suggests that Brazilian‐Portuguese translation is acceptable, understandable, reliable, and valid for evaluating the function in adolescents and young adults with osteosarcoma in lower extremity in Brazil. Pediatr Blood Cancer 2008;50:1039–1042. © 2007 Wiley‐Liss, Inc.
Paired intervention model for identified alcohol misuse in secondary care
Authors: Sims John , Kristian Marc R , Pritchard Karen , Jones Linda 
Published: October 2007

AbstractHigh numbers of individuals with identified alcohol misuse are being admitted to the secondary care setting. Once individuals are stabilized, from a physical treatment point of view, there is a ‘window of opportunity’ that presents itself from a psychological perspective. Once individuals are stabilized, they often do not require any acute care input but they stay in hospital in order to complete a cross‐tolerance prescribing regimen. Listening to the voice of the service user and considering their experience of care, this article discusses the merits of a ‘paired intervention’ response during this period. This refers to the concept of initiating psychological interventions to run in ‘tandem’ with chemically based treatments. Giving equal importance to psychological interventions delivered in conjunction with physical treatments is a way of optimizing this window of opportunity. Initiating a group‐based psychological response utilizing a motivational model of intervention can be an extremely facilitative experience within the in‐patient environment. The importance of a collaborative delivery of the message regarding the benefits to a paired intervention is discussed. Recognition from medical colleagues of the benefits to a paired intervention is acknowledged. A coordinated response is important if individuals are to be facilitated towards maintenance of positive behavioural change following hospital discharge. Copyright © 2007 John Wiley & Sons, Ltd.
Two‐dimensional separation of human plasma proteins using iterative free‐flow electrophoresis
Authors: Nissum Mikkel , Kuhfuss Sabine , Hauptmann Monika , Obermaier Christian , Sukop Ute , Wildgruber Robert , Weber Gerhard , Eckerskorn Christoph , Malmström Johan 
Published: October 2007

AbstractBlood plasma is the most complex human‐derived proteome, containing other tissue proteomes as subsets. This proteome has only been partially characterized due to the extremely wide dynamic range of the plasma proteins of more than ten orders of magnitude. Thus, the reduction in sample complexity prior to mass spectrometric analysis is particularly important and alternative separation methodologies are required to more effectively mine the lower abundant plasma proteins. Here, we demonstrated a novel separation approach using 2‐D free‐flow electrophoresis (FFE) separating proteins and peptides in solution according to their pI prior to LC‐MS/MS. We used the combination of sequential protein and peptide separation by first separating the plasma proteins into specific FFE fractions. Tryptic digests of the separated proteins were generated and subsequently separated using FFE. The protein separation medium was optimized to segregate albumin into specific fractions containing only few other proteins. An optimization of throughput for the protein separation reduced the separation time of 1 mL of plasma to approximately 3 h providing sufficient material for digestion and the subsequent peptide separation. Our approach revealed low‐abundant proteins (e.g., L‐selectin at 17 ng/mL and vascular endothelial‐cadherin precursor at 30 ng/mL) and several tissue leakage products, thus providing a powerful orthogonal separation step in the proteomics workflow.
Proteomics in free‐living Mus spretus to monitor terrestrial ecosystems
Authors: Montes‐Nieto Rafael , Fuentes‐Almagro Carlos A. , Bonilla‐Valverde Daniel , Prieto‐Álamo María‐José , Jurado Juan , Carrascal Montserrat , Gómez‐Ariza José Luis , López‐Barea Juan , Pueyo Carmen 
Published: October 2007

AbstractWe evaluated the suitability of high‐throughput proteomic methods to monitor terrestrial ecosystems. Free‐living Mus spretus from three sites along the “Domingo Rubio” (DR) stream were compared with mice from Doñana Biological Reserve (“Santa Olalla” lagoon (SOL) negative control), using specimens from an industrial settlement (phosphogypsum stacks (PS)) and rice fields (“Matochal” rice fields (ARZ)) as positive controls. Our 2‐DE analysis showed 36 spots with significantly altered expression. Sixteen were identified by MALDI‐TOF‐PMF and peptide matching with Mus musculus databases. Identified proteins play different roles: cytoskeletal dynamics, proteolysis, biotransformation, oxidative‐stress adaptation, and metabolism. Animals from different polluted environments showed contrasting differences in their proteomes, with specific increases and decreases in selected groups of proteins that seem to be co‐ordinately regulated. Proteomic data were consistent with metal biomonitoring and conventional biomarker responses, indicating that DR (and PS/ARZ) animals sustained a heavier pollutant burden than SOL specimens and suffered a chronic oxidative stress. Whereas some protein expression differences may protect mice from pollutant toxicity, others should make them more susceptible. Transcript expression signatures agree with the documented lack of correlation between mRNA and protein levels. Nonetheless, a positive significant correlation was found between the gpx1 mRNA molecules and the intensity of one of the two identified GPX1 isospots.
The cytoplasmic phosphoproteome of the Gram‐negative bacterium Campylobacter jejuni: Evidence for modification by unidentified protein kinases
Authors: Voisin Sébastien , Watson David C. , Tessier Luc , Ding Wen , Foote Simon , Bhatia Smita , Kelly John F. , Young N. Martin 
Published: October 2007

AbstractWe have undertaken a comprehensive analysis of cytoplasmic protein phosphorylation in Campylobacter jejuni by mass spectrometric identification of phosphoproteins and localization of the sites of modification by phosphopeptide analyses. Cell extracts, enriched for phosphoproteins using Fe(III) IMAC or commercial phosphoprotein purification kits, were analyzed by 1‐D and 2‐D SDS‐PAGE and subjected to mass fingerprinting by in‐gel tryptic digestion and MALDI‐TOF MS. Fifty‐eight phosphopeptides were identified from 1‐D gel bands by nano‐LC‐MS/MS and automated searching in a C. jejuni ORF database resulting in the unequivocal identification of 36 phosphoproteins of diverse function. In addition to elongation factors and chaperonins, which have been reported to be phosphorylated in other bacteria, the major phosphoproteins included bacterioferritin and superoxide dismutase. The sequences around the phosphorylated Ser and Thr residues are indicative of specific kinases being responsible for some of the modifications. However, many of the other identified proteins are enzymes that have phosphorylated substrates, including ATP, hence other modifications may arise from autophosphorylation. Comparative analyses of IMAC extracts from the Escherichia coli strain AD202 and Helicobacter pylori resulted in the identification of homologs of six of the C. jejuni phosphoproteins, though their overall phosphoproteome maps were distinctly different.
Evaluation of protein N‐glycosylation in 2‐DE: Erythropoietin as a study case
Authors: Llop Esther , Gallego Ricardo Gutiérrez , Belalcazar Viviana , Gerwig Gerrit J. , Kamerling Johannis P. , Segura Jordi , Pascual José A. 
Published: October 2007

AbstractThe structure, function, and physico‐chemical properties of many proteins are determined by PTM, being glycosylation the most complex. This study describes how a combination of typical proteomics methods (2‐DE) combines with glycomics strategies (HPLC, MALDI‐TOF‐MS, exoglycosidases sequencing) to yield comprehensive data about single spot‐microheterogeneity, providing meaningful information for the detection of disease markers, pharmaceutical industry, antidoping control, etc. Recombinant erythropoietin and its hyperglycosylated analogue darbepoetin‐α were chosen as showcases because of their relevance in these fields and the analytical challenge they represent. The combined approach yielded good results in terms of sample complexity (mixture glycoforms), reproducibility, sensitivity (∼25 pmoles of glycoprotein/spot), and identification of the underlying protein. Heterogeneity was present in all spots but with a clear tendency; spots proximal to the anode contained the highest amount of tetra‐antennary tetra‐sialylated glycans, whereas the opposite occurred for spots proximal to the cathode with the majority of the structures being undersialylated. Spot microheterogeneity proved a consequence of the multiple glycosylation sites as they contributed directly to the number of possibilities to account for a discrete charge in a single spot. The interest of this combined glycoproteomics method resides in the efficiency for detecting and quantifying subtle dissimilarities originated from altered ratios of identical glycans including N‐acetyl‐lactosamine repeats, acetylation, or antigenic epitopes, that do not significantly contribute to the electrophoretic mobility, but affect the glycan microheterogeneity and the potential underlying related functionality.
Induction of growth arrest and polycomb gene expression by reversine allows C2C12 cells to be reprogrammed to various differentiated cell types
Authors: Shan Sze Wan , Tang Mei Kuen , Chow Pak Ham , Maroto Miguel , Cai Dong Qing , Lee Kenneth K. H. 
Published: October 2007

AbstractReversine is a small, cell permeable synthetic chemical that has the ability to reprogram C2C12 myogenic cells to become various differentiated cell types. However, we still do not know how reversine works or the genes and proteins involved. Hence, we have used comparative proteomic techniques to address this issue. We have identified several proteins that were associated with cell cycle progression which were downregulated by reversine. Simultaneously, there were proteins associated with the induction of growth arrest that were upregulated. Consequently, we investigated the effects of reversine on C2C12 cell growth and established that it inhibited cell growth. Reversine had little affects on cell survival. We also investigated whether expressions of the polycomb genes, polycomb repressive complex 1 (PHC1) and Ezh2, were affected by reversine. Polycomb group genes are normally involved in chromatin based gene silencing. We found that PHC1 and Ezh2 expressions were enhanced by reversine and that it correlated with the inhibition of muscle specific transcriptional factor genes, myogenin, MyoD, and Myf5. Therefore, we believe that reversine is able to reprogram C2C12 cells to various differentiated cell types by inducing cell growth arrest, and promoting PHC1 and Ezh2 expressions.
Screening for distress in patients with brain cancer using the NCCN's rapid screening measure
Authors: Keir Stephen T. , Calhoun‐Eagan Roberta D. , Swartz Jonas J. , Saleh Oussama A. , Friedman Henry S. 
Published: October 2007

AbstractGoals of work: Patients with brain cancer are at a risk of experiencing elevated levels of distress due to the severe functional, neurocognitive, and neuropsychological sequelae of the disease. Using the National Comprehensive Cancer Network's Distress Thermometer, we evaluated the extent and sources of distress within a population of patients with brain cancer.Patients and methods: Participants were asked to complete the Distress Thermometer, a single‐item rapid screening tool for distress. The Distress Thermometer is a visual analog scale on which participants rate their level of distress from ‘0’ (none) to ‘10’ (extreme). Participants were also asked to designate which items from a 34‐item list constitute sources of distress.Main results: Fifty‐two percent of participants met the ⩾4 cut‐off score for distress. The scores were positively correlated with patient‐reported emotional sources of distress (r=0.444, p<0.001), physical sources of stress (r=0.231, p<0.05), and total number of concerns (r=0.368, p<0.001). On average, brain tumor patients reported 5.8 cancer‐related items of concern.Conclusion: Brain cancer patients are likely to experience distress at some point during their disease trajectory. Patient‐reported emotional sources of distress should be targeted and interventions should be designed to address sources of distress such as worry, sadness, and depression. Copyright © 2007 John Wiley & Sons, Ltd.
Effects of socioeconomic factors and cancer survivors' worries on their quality of life (QOL) in Japan
Authors: Kobayashi K. , Morita S. , Shimonagayoshi M. , Kobayashi M. , Fujiki Y. , Uchida Y. , Yamaguchi K. 
Published: October 2007

AbstractEffects of socioeconomic factors and cancer survivors' worries on their quality of life (QOL) were investigated. In 2002, Japanese national survey was performed to assess distress among cancer patients using a semi‐structured questionnaire (http://www.scchr.jp/yorozu/pdf/taiken_koe_eng.pdf). We investigated relationships between patients' distress and their QOL measured by European Organization for Research and Treatment of Cancer Core Questionnaire (EORTC QLQ‐C30) and Functional Assessment of Chronic Illness Therapy ‐ 12‐item Spiritual Well‐Being Scale (FACIT‐Sp), using a covariance structure analysis and multivariate regression analysis. A total of 130 outpatients (male: 42%; average age: 59 years; performance status rating 0–2:89%; breast/lung/gastrointestinal cancer: 38/22/21%) answered the questionnaires. A covariance structure analysis extracted latent variables, which were named socioeconomic distress and cancer worries, using a model that sufficiently represented the observed data (Goodness of fit index=0.833). Regression analysis demonstrated that higher family income significantly correlated with better Global health status/QOL (p=0.003) but that losing a job negatively correlated with all of the scales on functioning in the QLQ‐C30 (p<0.05) and spiritual well‐being (p<0.05). Patients' QOL was also affected by physical worries and spiritual issues in terms of emotional, cognitive, and social functioning. In conclusion, cancer survivors' QOL was doubly affected by socioeconomic distress and cancer worries. In the former, lower family income and losing employment by experiencing cancer had a negative impact on patients' QOL. As to the latter, physical worries and spiritual issues also affected patients' QOL. Copyright © 2007 John Wiley & Sons, Ltd.