bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are tactile function and body awareness of the foot related to motor outcomes in children with upper motor neuron lesions?
Authors: Marsico Petra , Meier Lea , van der Linden Marietta L. , Mercer Thomas H. , van Hedel Hubertus J. A. 
Published: March 2024

IntroductionSomatosensory function can be reduced in children with Upper Motor Neuron (UMN) lesions. Therefore, we investigated relationships between somatosensory functions of the foot and motor outcomes in children with UMN lesions.MethodIn this cross-sectional study, we assessed the Tactile Threshold (TT) with monofilaments and body awareness with Tactile Localisation Tasks for spatial-related action (TLTaction) and structural-related perception (TLTperception) body representation at the foot sole. Furthermore, we assessed four motor outcomes: the Selective Control Assessment of the Lower Extremity (SCALE), the modified Timed Up and Go test (mTUG), the Gillette Functional Assessment Questionnaire (FAQ), and the Functional Mobility Scale (FMS). Spearman's correlations (ρ) were applied to assess relationships between the somatosensory function of the foot sole and the applied motor outcomes.ResultsThirty-five children with UMN lesions, on average 11.7 ± 3.4 years old, participated. TLTperception correlated significantly with all lower limb motor outcomes (|ρ|=0.36–0.57; p &lt; 0.05), but TLTaction (|ρ|=0.00–0.27; p = 0.15–0.97, and TT did not (|ρ|=0.01–0.83; p = 0.73–0.94). TLTperception correlated strongly with the Gross Motor Function Classification System (|ρ|=0.62; p = 0.001) in children with cerebral palsy (n = 24).DiscussionAssessing structural body representation of the foot sole should be considered when addressing lower limb motor impairments, including gait, in children with upper motor neuron lesions. Our results suggest that the assessment of tactile function and spatial body representation may be less related to lower limb motor function.
Unidentified fish sounds as indicators of coral reef health and comparison to other acoustic methods
Authors: Jarriel Sierra D. , Formel Nathan , Ferguson Sophie R. , Jensen Frants H. , Apprill Amy , Mooney T. Aran 
Published: March 2024

The global decline of coral reefs is a major contributor to the global biodiversity crisis and requires improved monitoring at these critically important habitats. Non-invasive passive acoustic assessments may address this need, leveraging the rich variety and spatiotemporal variability of biological sounds present in coral reef environments and offering near-continuous temporal coverage. Despite this, acoustic metrics that reliably represent coral reef health are still debated, and ground-truthing of methods is limited. Here we investigated how the prevalence of low frequency biotic sounds (without species information) relates to coral reef health, providing a foundation from which one can compare assessment methods. We first quantified call rates of these low frequency sounds for three reefs exhibiting different community assemblages around St. John, U.S. Virgin Islands, by manually annotating presumed fish noises for 1 min every 30 min across 8 days for each site. Annotated days were selected at key points across lunar cycles. These call rates were then compared with traditional visual surveys, and several acoustic methods and indices commonly used in underwater soundscape research. We found that, overall, manually detected fish call rates successfully differentiated between the three reefs, capturing variation in crepuscular activity levels–a pattern consistent with previous work that highlights the importance of diel choruses. Moreover, fish vocal rates were predictors of hard coral cover, fish abundance, and fish species richness, while most acoustic indices failed to parse out fine distinctions among the three sites. Some, such as the Acoustic Complexity Index, failed to reveal any expected differences between sites or times of day, while the Bioacoustic Index could only identify the most acoustically active reef, otherwise having weak correlations to visual metrics. Of the indices tested, root-mean-squared sound pressure level and Acoustic Entropy, both calculated in the low frequency fish band (50–1,200 Hz), showed the strongest association with visual health measures. These findings present an important step toward using soundscape cues for reef health assessments. The limited generalizability of acoustic indices across different locations emphasizes the need for caution in their application. Therefore, it is crucial to improve methods utilizing fish sounds, such as automatic fish call detectors that are able to generalize well to new soundscapes.
Nutritional composition, phytochemicals, and antioxidant activities of Abies marocana Trab. needles
Authors: Zirari Malak , Aouji Marouane , Imtara Hamada , Hmouni Driss , Tarayrah Mahmoud , Noman Omar M. , El Mejdoub Nouredine 
Published: March 2024

IntroductionFinding natural sources of bioactive compounds is turning into a cutting- edge task for the scientific community and industry alike. Abies marocana, Moroccan fir, holds great importance due to its ecological, economic, social, and cultural significance.Material and MethodsThe current study aimed to evaluate the needles of Abies marocana in terms of its nutritional and anti-nutrient content, bioactive components, and antioxidant capacity. The AOAC technique was used to determine the composition of the needles. Mineral content was analyzed by inductively coupled plasma optical emission spectrometry. Phytochemical screening of methanol extract was performed using standard procedures, and multiple assays evaluated antioxidant activity. The extract’s volatile profile was elucidated using GC-MS method.Results and DiscussionThe presence of various components in the needles was discovered through proximate analysis, including carbohydrates, crude protein, crude fiber, crude fat, ash, and moisture. Anti-nutrients such as chlorophyll, carotenoids, and vitamin A were confirmed. Needles are a rich source of mineral elements and contain significant levels of phenols, flavonoids, tannins, and phytosterols. Palmitic acid, 17-octadecynoic acid, and (Z)-18- octadec-9-enolide were the main components identified by GC-MS analysis. The methanolic extract exhibited substantial antioxidant capacity. The DPPH scavenging activity displayed a remarkable percentage inhibition. The integration of Abies marocana needles into sustainable diets can contribute to the well- being of humans and the planet, since the nutrient and bioactive compounds present have potential applications in the pharmaceutical and food industries.
Case Report: Inflamed Jacobson nerve: an uncommon cause of persisting otalgia after an acute otitis media
Authors: Camurdan Aybige , Kilgue Alexander , Scholtz Lars Uwe , Todt Ingo 
Published: March 2024

IntroductionOtalgia can have multiple causes. Mostly otalgia is caused by a tubal dysfunction or an acute middle ear infection. This case describes a patient with an inflammation of the Jacobson's nerve causing severe persistent otalgia after an acute otitis media. The patients complaints completely disappeared after neurolysis of the Jacobson's nerve.Case presentationWe describe a case of a 21-year-old female caucasian patient with acute otitis media and persistent intractable otalgia. Infection was first successfully controlled by antibiotics. But the patient reported a persistent otalgia not responding to analgetics. We performed a CT scan, which exhibited a regular aerated middle ear finding, and a diagnostic tympanoscopy to examine the middle ear structures particularly the tympanic Jacobson's nerve as a possible cause for persistent pain. The following neurolysis of Jacobson's nerve under general anaesthesia led to a resolution of otalgia.ConclusionAn inflamed tympanic Jacobson's nerve is a rare observation and a persisting otalgia after an acute otitis media not responding to conservative treatment can be treated by a neurolysis.
Length-Weight-Age Relationship of Schizothorax eurystomus Kessler, 1872 and Comparison to Other Snow Trout Species in Central Asia
Authors: Karimov Erkin , Zeiringer Bernhard , Coeck Johan , Verhelst Pieterjan , Karimov Bakhtiyor , Omonov Otabek , Schletterer Martin , Hayes Daniel S. 
Published: March 2024

This study presents a comprehensive analysis of the length-weight relationship, condition factors, and age of Schizothorax eurystomus in the Shakhimardan River basin in Central Asia, along with a comparative perspective to other Schizothorax species in the region. The study found that S. eurystomus exhibits positive allometric growth, which is consistent with similar patterns observed in this species from the Syr Darya River basin. The two analyzed condition factors showed mean values within the normal range, indicating good feeding and environmental conditions. However, significant disparities between minimum and maximum values of these factors indicated varied growth conditions which may be influenced by anthropogenic factors. Age estimation using opercular bones showed variations in the total length among fish of the same age, and a clear age distribution pattern across different sites. Younger fish predominantly inhabited the shallower, warmer, and lower sections of the river, which is impacted by agricultural water diversion, while older specimens were found in areas with higher discharge and deeper pools. Overall, this research provides valuable insights into the life history traits of S. eurystomus, underlining the need for sustainable fishery management and conservation strategies in the Shakhimardan River basin. The findings also emphasize the importance of considering habitat quality and anthropogenic pressures regarding understanding both fish population dynamics and growth patterns.
Bacteriophage vB_SepP_134 and Endolysin LysSte_134_1 as Potential Staphylococcus-Biofilm-Removing Biological Agents
Authors: Golosova Natalia N. , Matveev Andrey L. , Tikunova Nina V. , Khlusevich Yana A. , Kozlova Yulia N. , Morozova Vera V. , Babkin Igor V. , Ushakova Tatiana A. , Zhirakovskaya Elena V. , Panina Elizaveta A. , Ryabchikova Elena I. , Tikunov Artem Y. 
Published: March 2024

Bacteria of the genus Staphylococcus are significant challenge for medicine, as many species are resistant to multiple antibiotics and some are even to all of the antibiotics we use. One of the approaches to developing new therapeutics to treat staphylococcal infections is the use of bacteriophages specific to these bacteria or the lytic enzymes of such bacteriophages, which are capable of hydrolyzing the cell walls of these bacteria. In this study, a new bacteriophage vB_SepP_134 (St 134) specific to Staphylococcus epidermidis was described. This podophage, with a genome of 18,275 bp, belongs to the Andhravirus genus. St 134 was able to infect various strains of 12 of the 21 tested coagulase-negative Staphylococcus species and one clinical strain from the Staphylococcus aureus complex. The genes encoding endolysin (LysSte134_1) and tail tip lysin (LysSte134_2) were identified in the St 134 genome. Both enzymes were cloned and produced in Escherichia coli cells. The endolysin LysSte134_1 demonstrated catalytic activity against peptidoglycans isolated from S. aureus, S. epidermidis, Staphylococcus haemolyticus, and Staphylococcus warneri. LysSte134_1 was active against S. aureus and S. epidermidis planktonic cells and destroyed the biofilms formed by clinical strains of S. aureus and S. epidermidis.
SISTEM PAKAR PENYAKIT KULIT MENGGUNAKAN METODE CERTAINTY FACTOR
Authors: Perangin angin Ratna Sari 
Published: March 2024

Seiring kemajuan ilmu pengetahuan dan teknologi khususnya dalam bidang kedokteran akan dihasilkan peralatan medis yang semakin canggih dalam memerangi penyakit atau melakukan deteksi lebih dini penyakit agar lebih cepat mendapat penanganan. Salah satunya adalah penyakit kulit pada manusia. Tujuan penelitian ini adalah membangun aplikasi sistem pakar yang mampu melakukan diagnosis penyakit kulit berdasarkan gejala yang dialami dengan mengimplementasikan metode Certainty Factor. Pengembangan sistem pakar menggunakan model waterfall, yang terdiri dari tahap analisis, merumuskan masalah, mengumpulkan data, dan pengujian. Implementasi metode Certainty Factor pada sistem pakar ini digunakan untuk menghasilkan tingkat kepercayaan dalam diagnosis penyakit. Sistem pakar diuji melalui 3 tahap pengujian yaitu uji blackbox, uji validitas dan uji pengguna. Pada uji blackbox, fungsi sistem berjalan sesuai yang diharapkan. Pada uji validitas, sistem dinyatakan valid untuk digunakan. Pada uji pengguna, responden memberikan kriteria “Layak”. Dari hasil penelitian dapat disimpulkan bahwa sistem mampu memberikan nilai kepercayaan dan dapat mendiagnosis penyakit kulit berdasarkan gejala yang dimasukkan user. Karena masih terbatasnya sistem, diharapkan adanya pengembangan sistem dengan metode lain sehingga lebih bermanfaat bagi pengguna.
SISTEM PENDUKUNG KEPUTUSAN PEMILIHAN SISWA BERPRESTASI DI SMP NEGERI 4 SATU ATAP PURBA DENGAN METODE AHP
Authors: Simarmata Ervin Riyanda 
Published: March 2024

Menjadi siswa berprestasi adalah impian setiap siswa di sekolah. Prestasi yang di dapat tentu didasarkan dengan suatu kemampuan terhadap pengetahuan yang dimiliki oleh masing-masing siswa. Kriteria yang ditetapkan dalam kasus ini adalah Nilai Raport, Absen, Sikap dan Extrakurikuler. Dari empat kriteria tersebut
akan menentukan siapa siswa berprestasi pada setiap tahun ajar. Sistem pendukung keputusan (SPK) merupakan suatu sistem yang interaktif, membantu pengambilan keputusan melalui penggunaaan data dan model-model keputusan untuk memecahkan masalah yang sifatnya semi terstruktur maupun yang tidak terstruktur. Perancanga dimulai dari diagram usecase, diagram activity, dan diagram sequence. Pembuatan aplikasi dalam hal ini menggunakan bahasa pemrograman Visual Basic 2010 dan menggunakan metode Analytic Hierarchy Process (AHP). Metode AHP (Analytic Hierarchy Proces) sesuai untuk diaplikasikan dengan menentukan nilai bobot dari semua kriteria setiap siswa sehingga pihak sekolah dapat mengambil keputusan menentukan siswa berprestasi tersebut.
SISTEM PENDUKUNG KEPUTUSAN PENILAIAN KINERJA DOSEN MENGGUNAKAN METODE HYBRID AHP DAN TOPSIS
Authors: Suarnatha I Putu Dody 
Published: March 2024

Untuk menghasilkan dosen yang berkompetensi dan profesional tentunya memerlukan berbagai upaya berkesinambungan serta terukur agar tujuan tersebut tercapai, salah satu upaya yang dapat dilakukan yaitu melalui penilaian kinerja dosen. Di Universitas Tabanan penilaian kinerja dosen dilaksanakan tiap akhir semester, namun dalam penyelenggaraanya ditemui kendala, yaitu: hasil penilaian yang masih belum sesuai, serta belum adanya tolak ukur penentuan kinerja dosen. Hal ini memiliki dampak pada proses evaluasi yang mengakibatkan hasil penilaian terkadang tidak efektif dan sering dilakukan tidak objektif dampak lainnya terletak pada proses pengambilan keputusan dalam merangking dan menentukan dosen yang memiliki kinerja yang baik ataupun kurang. Maka dari itu, untuk mengatasi kendala tersebut diperlukanlah suatu sistem pendukung keputusan (SPK). SPK yang dibangun menggunakan gabungan/hybrid antara metode AHP dan TOPSIS. Metode AHP digunakan pada proses pembobotan kriteria sedangkan metode TOPSIS untuk perhitungan perangkingan. Sistem pendukung keputusan yang dibangun menggunakan tiga kriteria yaitu : Pendidikan, Penelitian danPengabdian Kepada Masyarakat atau lebih dikenal dengan nama Tri Dharma Perguruan Tinggi.
Engineering of the AAV-Compatible Hair Cell-Specific Small-Size Myo15 Promoter for Gene Therapy in the Inner Ear
Authors: Hu Shao Wei , Lv Jun , Wang Zijing , Tang Honghai , Wang Hui , Wang Fang , Wang Daqi , Zhang Juan , Zhang Longlong , Cao Qi , Chen Yuxin , Gao Ziwen , Han Yu , Wang Wuqing , Li Geng-lin , Shu Yilai , Li Huawei 
Published: March 2024

Adeno-associated virus (AAV)-mediated gene therapy is widely applied to treat numerous hereditary diseases in animal models and humans. The specific expression of AAV-delivered transgenes driven by cell type-specific promoters should further increase the safety of gene therapy. However, current methods for screening cell type-specific promoters are labor-intensive and time-consuming. Herein, we designed a “multiple vectors in one AAV” strategy for promoter construction in vivo. Through this strategy, we truncated a native promoter for Myo15 expression in hair cells (HCs) in the inner ear, from 1,611 bp down to 1,157 bp, and further down to 956 bp. Under the control of these 2 promoters, green fluorescent protein packaged in AAV-PHP.eB was exclusively expressed in the HCs. The transcription initiation ability of the 2 promoters was further verified by intein-mediated otoferlin recombination in a dual-AAV therapeutic system. Driven by these 2 promoters, human otoferlin was selectively expressed in HCs, resulting in the restoration of hearing in treated

Otof
−/−

mice for at least 52 weeks. In summary, we developed an efficient screening strategy for cell type-specific promoter engineering and created 2 truncated Myo15 promoters that not only restored hereditary deafness in animal models but also show great potential for treating human patients in future.
Усереднення в математичних моделях під дією багаточастотних збурень із запізненням
Authors: Бігун Ярослав Йосипович , Українець Олег Захарович , Скутар Ігор Дмитрович 
Published: March 2024

Метод усереднення за швидкими змінними застосовано для дослідження математичних моделей природничих процесів з лінійними запізненнями під дією багаточастотних збурень. Побудовано усереднену систему за швидкими змінними, яка значно простіша точної системи рівнянь. Доведено існування і єдиність неперервно диференційовного розв’язку на скінченному часовому відрізку. Обґрунтування методу усереднення будується на оцінках осциляційних інтегралів, відповідних багаточастотній системі. Напрям досліджень багаточастотних систем запропоновано у працях А.М. Самойленка і Р.І. Петришина. Диференціальним рівнянням із запізненням аргументу і початковими, багатоточковими й інтегральними умовами, дослідженню за допомогою методу усереднення систем, які в процесі еволюції проходять через резонанс, присвячені праці Я.Й. Бігуна. Встановлено умову резонансу, яка залежить не тільки від частот, але і від запізнень у швидких змінних. Одержана оцінка методу усереднення явно залежить від малого параметра та кількості швидких змінних і запізнень у них. Асимптотика оцінки є непокращуваною при накладених у роботі умовах. Основною умовою побудови оцінки є умова виходу системи з малого околу резонансу. У роботі такою умовою є відмінність від нуля на часовому відрізку визначника Вронського порядку побудованого за системою векторів частот з лінійно перетвореними аргументами, — кількість швидких змінних і частот. Одержаний результат проілюстровано на моделі Вольтерри–Лотки під впливом одночастотного збурення з лінійно перетвореним аргументом. Асимптотика оцінки похибки методу має порядок і характерна для двочастотної системи без запізнення. Для усередненої задачі проаналізовано біфуркації стану рівноваги, який відповідає співіснуванню двох видів. Результати роботи можна застосувати при побудові і дослідженні математичних моделей динаміки популяції за наявності багаточастотних збурень. Такі дослідження актуальні в період воєнних дій, коли на процеси у природі впливають шуми, світлові, механічні і електромагнітні збурення. Також одержаний результат заслуговує на увагу в моделях поширення епідемій, імунної відповіді організму при інфекційних захворюваннях, при дослідженні політичних і воєнних конфліктів.
Clinical Diagnosis of Polyglandular Autoimmune Syndrome
Authors: Янголенко В.В. , Негурко М.В. , Тимофеева Т.В. , Гребенникова И.В. 
Published: March 2024

В статье представлены клинические проявления аутоиммунного полигландулярного синдрома и влияние их на качество жизни.
The article presents clinical aspects of polyglandular autoimmune syndrome according to the time intervals of the disease and stages of their manifestation in the course of the life.
Modern Approaches of Detection and Correction of Menopausal Syndrome in Clinical Practice
Authors: Шепелькевич А.П. , Лузан А.М. , Шкарупа А.С. , Кудинова Е.В. 
Published: March 2024

Менопаузальный период является физиологическим этапом репродуктивной жизни женщины. При этом термин «менопауза» в настоящее время определяется как последняя менструация, возникшая вследствие естественного или индуцированного снижения активности яичников. В 30–40% случаев менопаузальный период сопровождается патологическими проявлениями, существенно снижающими качество жизни женщины. В таком случае речь идет о менопаузальном или климактерическом синдроме или климактерии. Согласно современным рекомендациям диагностика менопаузального синдрома проводится на основании клинических данных без привлечения лабораторного тестирования. Тактика лечения менопаузальных симптомов вырабатывается вместе с пациенткой и включает модификацию образа жизни, отказ от вредных привычек, негормональную медикаментозную и когнитивно-поведенческую терапию. При принятии решения о назначении МГТ необходимо учитывать противопоказания и критерии для назначения данной терапии с целью снижения риска смертности и улучшения качества жизни женщины. В статье представлено патофизиологическое обоснование своевременного выявления и коррекции менопаузального (климактерического) синдрома.
The menopausal period is a physiological stage of a woman’s reproductive life. Moreover, the term "menopause" is currently defined as the last menstruation that occurs as a result of a natural or induced decrease in ovarian activity. In 30–40% of cases the menopausal period is accompanied by pathological manifestations that significantly reduce a woman’s quality of life. In this case, it can be called menopausal or climacteric syndrome. According to the modern recommendations, the diagnosis of the menopausal condition should be carried out on the basis of clinical data without the involvement of laboratory testing, that can be facilitating and at the same time challenging. This article presents pathophysiological reasons for timely detection and correction of the menopausal (climacteric) syndrome.
Diabetic Hypoglycemia and its Consequences in Assessing the Quality of Management of Diabetes Mellitus
Authors: Данилова Л.И. , Короленко Г.Г. , Лущик М.Л. , Романовский А.А. , Бурко И.И. , Исачкина О.Н. 
Published: March 2024

В статье дается обзор основных положений, касающихся диабетической гипогликемии. Рассматриваются клинические проявления и причины гипогликемии и гипогликемической комы при сахарном диабете (СД). Приводятся классификации гипогликемий, предложенные Американской диабетической ассоциацией (ADA) и российскими «Алгоритмами специализированной помощи больным сахарным диабетом». Показана распространенность гипогликемий у пациентов с СД1 и СД2 по данным крупных международных исследований. Рассматриваются основные механизмы развития бессимптомной гипогликемии. Особый акцент делается на описании последствий гипогликемических состояний в оценке качества ведения сахарного диабета. На основании собственного клинического опыта описываются ситуации, в которых наиболее часто наблюдается симптомная гипогликемия 2–3-го уровня. Выделяются приоритеты при управлении гипогликемией.
The article presents literature review on the problem of diabetic hypoglycemia. The symptoms and causes of hypoglycemia and hypoglycemic coma in patients with diabetes mellitus are considered. The classifications of hypoglycemia proposed by the American Diabetes Association (ADA) and Russian Standards of specialized diabetes care are given. The frequency of hypoglycemia in patients with type 1 and type 2 diabetes is shown. The mechanisms involved in the development of asymptomatic hypoglycemia are described. Special attention is paid to consequences of hypoglycemic episodes in assessing the quality of management of diabetes mellitus. Risk factors for symptomatic level 2 and level 3 hypoglycemia are determined based on our own clinical experience. Priorities in the management of hypoglycemia are highlighted.