bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cloning, expression, and localization of a molt‐related β‐N‐acetylglucosaminidase in the spruce budworm, Choristoneura fumiferana
Authors: Zheng Y.‐P. , Krell P.J. , Doucet D. , Arif B.M. , Feng Q.‐L. 
Published: March 2008

AbstractA β‐N‐acetylglucosaminidase cDNA (CfGlcNAcase) was cloned from the spruce budworm, Choristoneura fumiferana. Western blotting analysis of developmental CfGlcNAcase expression revealed high levels of expression of the gene on the last day of the 5th instar larvae and the first day in the 6th instar larvae, followed by a decrease to background levels during the intermolt of the 6th instar. CfGlcNAcase was detected again from the last day of the 6th instar to day 2 of pupal stage. CfGlcNAcase expression was induced by tebufenozide at 24 h post treatment and remained at high levels until 72 h. Immunohistochemical localization analysis of CfGlcNAcase indicated that CfGlcNAcase was present in the molting fluid, epidermis, trachea, and hemolymph in prepupae during the transformation from larva to pupa. CfGlcNAcase cDNA was expressed into a recombinant protein in bacterial and baculovirus systems and the protein expressed in the baculovirus system had a higher chitinolytic activity than in the bacterial system and appeared to be secreted. Arch. Insect Biochem. Physiol. 2008. © 2008 Wiley‐Liss, Inc.
Efficient Synthesis of Symmetrically and Unsymmetrically Substituted Hexaphenylbenzene Analogues by Suzuki–Miyaura Coupling Reactions
Authors: Yang Xiaoyin , Dou Xi , Müllen Klaus 
Published: March 2008

AbstractA series of symmetrically and unsymmetrically substituted hexaphenylbenzene (HPB) analogues were efficiently synthesized by using a sterically hindered Suzuki–Miyaura cross‐coupling reaction of arylboronic acids with 1,4‐diiodo‐2,3,5,6‐tetraarylbenzenes under our optimized reaction conditions. The 1,4‐diiodo‐2,3,5,6‐tetraarylbenzenes can be readily prepared by using a one‐pot Hart reaction. Oxidative cyclodehydrogenation of the dibromo derivative with FeCl3 gave the corresponding hexabenzocoronene (HBC) derivative in good yield.
TROSY effects in MAS solid‐state NMR
Authors: Chevelkov Veniamin , Reif Bernd 
Published: March 2008

AbstractUse of transverse relaxation‐optimized spectroscopy (TROSY) type techniques had a dramatic impact on the study of large proteins with a molecular weight >30kDa for solution‐state NMR. In the solid‐state, such an effect would not be expected a prior, as the investigated molecules are immobilized. However, local motions induce fluctuations of the local fields experienced by the nuclear spins and, this way, are effective for relaxation. We demonstrate that protein dynamics can significantly influence the resonance line width in ultra high resolution MAS (magic angle spinning) solid‐state NMR experiments. Averaging of the 15NHα/β multiplet components as a consequence of 1H decoupling induces effective broadening of the 15N resonance. Application of TROSY type techniques that select only the narrow component of the multiplet pattern results in an increased resolution and, thus, will be of benefit for MAS solid‐state NMR spectroscopy. © 2008 Wiley Periodicals, Inc. Concepts Magn Reson Part A 32A: 143–156, 2008.
A Water‐Stable and Strongly Luminescent Terbium(III) Macrocyclic Complex Incorporating an Intracyclic Pyrazolylpyridine Chromophore
Authors: Nasso Isabelle , Bedel Sébastien , Galaup Chantal , Picard Claude 
Published: March 2008

AbstractA 15‐membered macrocyclic ligand L1H3 was designed with the aim of sensitizing the visible luminescence of the TbIII ion in water: this octadentate chelating ligand is based on an intracyclic N,C‐pyrazolylpyridine chromophore and a diethylenetriaminetriacetic acid core. This ligand reacts with equimolar amounts of LnCl3 salts to yield water‐soluble and stable complexes of the general formula [L1·Ln(H2O)]0. Detailed photophysical studies were performed in aerated water on the free ligand and its GdIII complex and complexes formed with LnIII ions commonly used in fluoroimmunoassays (Ln = Sm, Eu, Tb, and Dy). The results show a wide range in the emission properties of the complexes, which can be rationalized in terms of the chromophore triplet‐state energy. In particular, the TbIII complex has a remarkable overall quantum yield of ca. 50 % in H2O (80 % in D2O) and an energy‐transfer efficiency from sensitizer to the terbium ion of ca. 85 %. According to these results and in view of its kinetic inertness in aqueous solutions, the L1·Tb complex may be considered as a very promising candidate as long‐lived luminescent label for bioaffinity assays. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Enantiocontrolled Preparation of the First Stable α‐Ferrocenylalanine Derivatives
Authors: Moreno Rosa M. , Catasús Mònica , López Concepción , Moyano Albert 
Published: March 2008

AbstractAn enantiocontrolled synthesis of α‐ferrocenylalanine methyl ester (S)‐24 and of the corresponding N‐Boc derivative (S)‐25 is presented. We have found that an important source of instability for α‐ferrocenyl‐α‐amino acid derivatives is the high reactivity of the α‐hydrogen, and its replacement by a methyl group has proven to be essential for the stability of these compounds. The synthesis of (S)‐24 requires only six steps from the readily available 2‐propenylferrocene which is converted into the diol (S)‐16 by Sharpless catalytic dihydroxylation. Regio‐ and stereoselective azide substitution of the tertiary hydroxy group affords the azido diol (S)‐17. Swern oxidation gives the corresponding aldehyde which is smoothly oxidized to the carboxylic ester by means of the Smith–Kozlowski protocol. Transesterification and azide reduction lead to the α‐amino ester (S)‐24 in a good overall yield and enantiomeric purity. A cyclic voltammetric study of this compound showed that oxidation takes place by a simple reversible one‐electron‐transfer process. Half a century after the initial unsuccessful attempts to obtain ferrocenylglycine, this is the first synthesis of a stable α‐amino acid derivative incorporating a ferrocene unit at the α‐position.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Development of Prototype Wireless Transmission Measurement for Glucose in Subcutaneous and Brain Striatum
Authors: Ahmad Farook , Chow Fook Cheong , Ho Yah Wen , Chin Yew Siang , Christenson Andreas , Bainbridge Martina , Md Yusof Ahmad Pauzi , Ab Ghani Sulaiman 
Published: March 2008

AbstractMonitoring of glucose in subcutaneous and brain striatum have been extensively studied in the past. While biocompatibility was one of the limitations, others included the messy measuring equipments preclude monitoring in a complex environment. This study tried to establish an amperometric measurement of glucose in pre‐ and post‐insulin‐administration on diabetic and hyperglycemia rats via wireless. The results have indicated that the wireless sensing kit used was capable of monitoring glucose in both subcutaneous and brain. The physiological data have also shown a new insight on the fabrication of implantable glucose sensors.
Al(III) and Fe(III) Balance in Hemodialysis Treatment Assessed via Fluid Analysis by Adsorptive Stripping Voltammetry and UV Sample Digestion
Authors: Nascimento Paulo C. , Del‐Fabro Luciana D. , Bohrer Denise , de Carvalho Leandro M. , Rosa Marcelo B. , Noremberg Simone M. 
Published: March 2008

AbstractSimultaneous determination of Al(III) and Fe(III) in posthemodialysis fluids was investigated by the Adsorptive stripping voltammetry of solochrome violet metal complexes. The adsorption of the complexes on the mercury electrode (HMDE) was investigated by out of phase altenating current voltammetry in presence of the main matrix interfering species. Sample digestion by UV irradiation was investigated to overcome the matrix interference. The proposed method was valid for real posthemodialysis samples containing or not Desferrioxamine B. Detection limits of 1.4 and 1.8 μg L−1 were calculated for Al(III) and Fe(III), respectively. Recoveries ranging from 88.1 to 106.3% were obtained from spiking experiments.
Developmental differences of neurocognitive networks for phonological and semantic processing in Chinese word reading
Authors: Cao Fan , Peng Danling , Liu Li , Jin Zhen , Fan Ning , Deng Yuan , Booth James R. 
Published: March 2008

AbstractDevelopmental differences in the neurocognitive networks for phonological and semantic processing in Chinese word reading were examined in 13 adults and 13 children using functional magnetic resonance imaging (fMRI). Rhyming and semantic association judgments were made to two‐character words that were presented sequentially in the visual modality. These lexical tasks were compared with a nonlinguistic control task involving judgment of line patterns. The first main finding was that adults showed greater activation than children in right middle occipital gyrus on both the meaning and rhyming task, suggesting adults more effectively engage right hemisphere brain regions involved in the visual‐spatial analysis of Chinese characters. The second main finding was that adults showed greater activation than children in left inferior parietal lobule for the rhyming as compared with the meaning task, suggesting greater specialization of phonological processing in adults. The third main finding was that children who had better performance in the rhyming task on characters with conflicting orthographic and phonological information relative to characters with nonconflicting information showed greater activation in left middle frontal gyrus, suggesting greater engagement of brain regions involved in the integration of orthography and phonology. Hum Brain Mapp, 2009. © 2008 Wiley‐Liss, Inc.
The role of viral and bacterial pathogens in gastrointestinal cancer
Authors: Selgrad Michael , Malfertheiner Peter , Fini Lucia , Goel Ajay , Boland C. Richard , Ricciardiello Luigi 
Published: March 2008

AbstractThe association of Helicobacter pylori (H. pylori) with gastric cancer is thus far the best understood model to comprehend the causal relationship between a microbial pathogen and cancer in the human gastrointestinal tract. Besides H. pylori, a variety of other pathogens are now being recognized as potential carcinogens in different settings of human cancer. In this context, viral causes of human cancers are central to the issue since these account for 10–20% of cancers worldwide. In the case of H. pylori and gastric cancer, as well as the human papillomavirus and anal cancer, the causal relationship between the infectious agent and the related cancer in the gastrointestinal tract has been clearly confirmed by epidemiological and experimental studies. Similarly, Epstein–Barr virus and the oncogenic JC virus are being suggested as possible causative agents for cancers in the upper and lower gastrointestinal tract. This review discusses various viral and microbial pathogens and their oncogenic properties in the evolution of gastrointestinal carcinogenesis and summarizes the available experimental data make a convincing agreement favoring the associations between infectious agents and specific human cancers. J. Cell. Physiol. 216: 378–388, 2008. © 2008 Wiley‐Liss, Inc.
P53 mutations in colorectal cancer from northern Iran: Relationships with site of tumor origin, microsatellite instability and K‐ras mutations
Authors: Mahdavinia Mahboobeh , Bishehsari Faraz , Verginelli Fabio , Cumashi Albana , Lattanzio Rossano , Sotoudeh Masoud , Ansari Reza , Semeraro Daniela , Hormazdi Mahshid , Fakheri Hafez , Rakhshani Naser , De Lellis Laura , Curia Maria Cristina , Cama Alessandro , Piantelli Mauro , Malekzadeh Reza , Iacobelli Stefano , Mariani‐Costantini Renato 
Published: March 2008

AbstractCRC‐associated P53 mutations have not been studied extensively in non‐Western countries at relatively low CRC risk. We examined, for the first time, 196 paraffin‐embedded CRC cases from Northern Iran for mutations in P53 exons 5–8 using PCR‐direct sequencing. P53 status and mutation site/type were correlated with nuclear protein accumulation, clinicopathologic variables and data on K‐ras mutations and high‐level microsatellite instability (MSI‐H). We detected 96 P53 mutations in 87 (44.4%) cases and protein accumulation in 84 cases (42.8%). P53 mutations correlated directly with stage and inversely with MSI‐H. Distal CRCs were more frequently mutated at major CpG hotspot codons [248 (8/66, 12.1%), 175 (7/66, 10.6%), and 245 (7/66, 10.6%)], while in proximal tumors codon 213, emerged as most frequently mutated (5/28, 17.9% vs. 3/66, 4.5%, P = 0.048). Transitions at CpGs, the most common mutation type, were more frequent in non‐mucinous (25% vs. 10.4% in mucinous, P = 0.032), and distal CRC (27% vs. 12.5% in proximal, P = 0.02), and correlated with K‐ras transversions. Transitions at non‐CpGs, second most common P53 mutation, were more frequent in proximal tumors (15.6% vs. 4.7% in distal, P = 0.01), and correlated with K‐ras transitions and MSI‐H. Overall frequency and types of mutations and correlations with P53 accumulation, stage and MSI‐H were as reported for non‐Iranian patients. However P53 mutation site/type and correlations between P53 and K‐ras mutation types differed between proximal and distal CRC. The codon 213 P53 mutation that recurred in proximal CRC was previously reported as frequent in esophageal cancer from Northern Iran. J. Cell. Physiol. 216: 543–550, 2008. © 2008 Wiley‐Liss, Inc.
Pyrin, product of the MEFV locus, interacts with the proapoptotic protein, Siva
Authors: Balci‐Peynircioglu Banu , Waite Andrea L. , Hu Chunbo , Richards Neil , Staubach‐Grosse Ann , Yilmaz Engin , Gumucio Deborah L. 
Published: March 2008

AbstractMutations in pyrin cause the autoinflammatory disorder familial Mediterranean fever (FMF), a syndrome characterized by sporadic and unpredictable attacks of fever and localized severe pain. Currently, it is not clear how attacks are triggered, nor why they spontaneously resolve after 2 or 3 days. In fact, the cellular function of the pyrin protein and the molecular underpinnings of its malfunction in FMF have so far eluded clear definition. The identification of pyrin‐interacting proteins has the potential to increase our understanding of the cellular networks in which pyrin functions. Previous reports have established that pyrin interacts with the apoptotic protein ASC, the cytoskeletal adaptor protein PSTPIP1, the inflammatory caspase, Caspase‐1 and certain forms of the cytosolic anchoring protein 14‐3‐3. Here, we report that pyrin also interacts with Siva, a pro‐apoptotic protein first identified for its interaction with the cytosolic tail of CD27, a TNF family receptor. The interaction between pyrin and Siva involves the C‐terminal B30.2/rfp/SRPY domain of pyrin and exon 1 of Siva. We show that Siva and pyrin are indeed co‐expressed in human neutrophils, monocytes, and synovial cells. Furthermore, using a novel protein/protein interaction assay, we demonstrate that pyrin can recruit Siva to ASC specks, establishing a potential platform for intersection of ASC and Siva function. Finally, we show that pyrin modulates the apoptotic response to oxidative stress mediated by Siva. Thus, the Siva–pyrin interaction may be a potential target for future therapeutic strategies. J. Cell. Physiol. 216: 595–602, 2008, © 2008 Wiley‐Liss, Inc.
Characterization of Ni(II) complexes of Schiff bases of amino acids and (S)‐N‐(2‐benzoylphenyl)‐1‐benzylpyrrolidine‐2‐carboxamide using ion trap and QqTOF electrospray ionization tandem mass spectrometry
Authors: Jirásko Robert , Holčapek Michal , Kolářová Lenka , Nádvorník Milan , Popkov Alexander 
Published: March 2008

AbstractThis work demonstrates the application of electrospray ionization mass spectrometry (ESI‐MS) using two different mass analyzers, ion trap and hybrid quadrupole time‐of‐flight (QqTOF) mass analyzer, for the structural characterization of Ni(II) complexes of Schiff bases of (S)‐N‐(2‐benzoylphenyl)‐1‐benzylpyrrolidine‐2‐carboxamide with different amino acids. ESI enables the determination of molecular weight on the basis of rather simple positive‐ion ESI mass spectra containing only protonated molecules and adducts with sodium or potassium ions. Fragmentation patterns are characterized by tandem mass spectrometric experiments, where both tandem mass analyzers provide complementary information. QqTOF data are used for the determination of elemental composition of individual ions due to mass accuracies always better than 3 ppm with the external calibration, while multistage tandem mass spectra obtained by the ion trap are suitable for studying the fragmentation paths. The novel aspect of our approach is the combination of mass accuracies and relative abundances of all isotopic peaks in isotopic clusters providing more powerful data for the structural characterization of organometallic compounds containing polyisotopic elements. The benefit of relative and absolute mean mass accuracies is demonstrated on the example of studied Ni(II) complexes. Copyright © 2008 John Wiley & Sons, Ltd.
Classification and geometric aspects of vector valued Fourier transforms
Authors: Park In Sook 
Published: March 2008

AbstractIt is shown that for any locally compact abelian group 𝔾 and 1 ≤ p ≤ 2, the Fourier type p norm with respect to 𝔾 of a bounded linear operator T between Banach spaces, denoted by ‖T |ℱ𝒯𝔾p‖, satisfies ‖T |ℱ𝒯𝔾p‖ ≤ ‖T |ℱ𝒯𝔸p‖, where 𝔸 is the direct product of ℤ2, ℤ3, ℤ4, … It is also shown that if 𝔾 is not of bounded order then Cnp ‖T |ℱ𝒯𝕋p‖ ≤ ‖T |ℱ𝒯𝔾p‖, where 𝕋 is the circle group, n is a onnegative integer and Cp = . From these inequalities, for any locally compact abelian group 𝔾 ‖T |ℱ𝒯𝔾2‖ ≤ ‖T |ℱ𝒯𝕋2‖, and moreover if 𝔾 is not of bounded order then ‖T |ℱ𝒯𝔾2‖ = ‖T |ℱ𝒯𝕋2‖. The Hilbertian property and B‐convexity are discussed in the framework of Fourier type p norms. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Complexity penalized least squares estimators: Analytical results
Authors: Wittich O. , Kempe A. , Winkler G. , Liebscher V. 
Published: March 2008

AbstractA simple variational approach to the estimation of timeseries is studied in detail and mathematical rigor. The functional in question is a complexity penalized sum of squares. The results include existence and uniqueness of the statistical estimate, as well as continuous dependence and stability in dependence on parameters and data. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Some preconditioners for the CFIE equation of electromagnetism
Authors: Levadoux David P. 
Published: March 2008

AbstractWe present three weak parametrices of the operator of the combined field integral equation (CFIE). An interesting feature of these parametrices is that they are compatible with different discretization strategies and hence allow for the construction of efficient preconditioners dedicated to the CFIE. Their numerical analysis shows that a regularization process acting at the continuous level of the equation is also effective at the discrete level if the mesh size tends to zero. First numerical tests confirm this effect and preconditioning is observed indeed. Furthermore, we show that the underlying operator of CFIE satisfies a uniform discrete Inf–Sup condition that allows one to predict an original convergence result for the numerical solution of CFIE to the exact one. Copyright © 2008 John Wiley & Sons, Ltd.
Molecular thermodynamic model of the glass transition temperature: dependence on molecular weight
Authors: Kim Yong Woo , Park Jung Tae , Koh Joo Hwan , Min Byoung Ryul , Kim Jong Hak 
Published: March 2008

AbstractA new molecular thermodynamic model to predict the dependency of the glass transition temperature (Tg) of polymer on its molecular weight was developed based on the configurational entropy model and the Flory–Huggins theory. In this model, the disorientation entropy of the polymer (Sdis) has been taken into account. Quantitative descriptions according to the proposed model are consistent with experimental Tg data of several polymers against the number of chain segment (r). At the same Tg.∞ (Tg of polymer at a infinite r value), the degree of polymer disorientation is strongly correlated with the slope of straight line at lower r regions in the Tg versus r plot, which is quantitatively identified by physical parameter (γdis) in this model. Copyright © 2008 John Wiley & Sons, Ltd.
Association between medication and risk of suicide, attempted suicide and death in nationwide cohort of suicidal patients with schizophrenia
Authors: Haukka Jari , Tiihonen Jari , Härkänen Tommi , Lönnqvist Jouko 
Published: March 2008

AbstractPurposeMortality among schizophrenia patients is substantially higher than in the general population. The aim of this study was to investigate, in a nationwide cohort of suicidal schizophrenic individuals, how the risks of suicide, severe suicide attempts and death are associated with usage of antidepressant or antipsychotic treatment.MethodsThe study population included all individuals in Finland who were hospitalised with a diagnosis of attempted suicide between 1 January 1997 and 31 December 2003, who also had at least one hospitalisation due to schizophrenia diagnosis (ICD‐10 F20), and were at least 16 years old when the index hospitalisation began. Cox's proportional hazards modelling and Bayesian intensity estimation were used in the analysis.ResultsThere were 1611 patients with a mean follow‐up time of 4.3 years. Current use of antipsychotics was associated with decreased mortality due to suicide (HR 0.52, 95% CI 0.34–0.81, p = 0.004), but no significant decrease in mortality was observed during current use of antidepressants (0.66, 0.41–1.08, p = 0.099), when compared to past use. In more detailed analysis when current users were compared to non‐users, olanzapine, and mixed use of antipsychotics, were associated with reduced all‐cause mortality, and mixed use also with reduced risk of suicide mortality. Current use of citalopram was associated with decreased all‐cause and suicide mortality.ConclusionsIn a population of suicidal schizophrenic individuals antipsychotic medication, treatment was associated with lower mortality from suicide and all‐causes. Antidepressive medication was associated with lower all‐cause mortality when used in combination with antipsychotics. Copyright © 2008 John Wiley & Sons, Ltd.
Structural and enzymatic characterization of HP0496, a YbgC thioesterase from Helicobacter pylori
Authors: Angelini Alessandro , Cendron Laura , Goncalves Susana , Zanotti Giuseppe , Terradot Laurent 
Published: March 2008

AbstractYbgC proteins are bacterial acyl‐CoA thioesterases associated with the Tol‐Pal system. This system is important for cell envelope integrity and is part of the cell division machinery. In E. coli, YbgC associates with the cell membrane and is part of a protein network involved in lipid biogenesis. In the human pathogen Helicobacter pylori, a putative homologue of YbgC, named HP0496, was found to interact with the cytotoxin CagA by two different studies. We have determined its crystal structure and characterized its enzymatic activity. The structure of HP0496 shows that it is a member of the hot‐dog family of proteins, with a εγ tetrameric arrangement. Finally, enzymatic assays performed with the purified protein showed that HP0496 is an acyl‐CoA thioesterase that favors long‐chain substrates. Proteins 2008. © 2008 Wiley‐Liss, Inc.
Response to András Szilágyi's Commentary
Authors: Zbilut Joseph P. , Giuliani Alessandro , Webber Charles L. 
Published: March 2008

AbstractIn a commentary to our article on protein domain size, András Szilágyi suggests our findings are a mathematical artifact. In examining his concerns, we believe they are possibly a result of misunderstandings, errors of fact, and philosophical approach. Proteins 2008. © 2008 Wiley‐Liss, Inc.