bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chiral phthalimides against penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus: molecular docking and in vitro analysis
Authors: Khan Aamina Azam , Khan Momin , Khan Sher Wali , Siddique Nayyer , Abid Rimsha , Zulfiqar Shandana , Rahman Sidra , Ali Muhammad 
Published: February 2024

Staphylococcus aureus (S. aureus) is a commensal bacterium and an opportunistic pathogen causing a wide variety of infections ranging from localized skin and soft tissue infections to life-threatening severe bacteremia, osteomyelitis, endocarditis, atopic dermatitis, prosthetic joint infection, staphylococcal food poisoning, medical device-related infections, and pneumonia. It is attributed to an acquired resistant gene, mecA, encoding penicillin-binding protein 2a (PBP2a). PBP2a is an essential protein responsible for the resistivity of methicillin-resistant S. aureus (MRSA) to various beta-lactam antibiotics. The antimicrobial treatment alternatives for MRSA are increasingly limited. Therefore, developing alternative therapeutic options for its treatment is the need of the day. Phthalimides and their N-substituted derivatives are of biological importance as they possess extensive biological and pharmaceutical properties and can serve as an excellent therapeutic option for MRSA. This study uses three chiral phthalimides (FIA, FIB, and FIC) to check their in silico and in vitro inhibitory effects. Molecular docking of these chiral phthalimides against PBP2a of MRSA was performed initially. After promising results, these novel compounds were screened through the agar-well diffusion method and micro-broth dilution assay to investigate their in vitro inhibitory activities with FIB being the strongest anti-staphylococcal agent yielding a 21 mm zone of inhibition and a minimum inhibitory concentration (MIC) of 0.022 ug, respectively. The zones of inhibition obtained through the in vitro activity showed that these chiral phthalimides possess substantial anti-MRSA activities and have the potential to be considered as alternative chemotherapeutics to treat the infections caused by MRSA after the confirmation of their cytotoxic and pharmacokinetic studies.
Evaluating the effectiveness of a novel somatostatin receptor 2 antagonist, ZT-01, for hypoglycemia prevention in a rodent model of type 2 diabetes
Authors: D’Souza Ninoschka C. , Aiken Julian A. , Hoffman Emily G. , Atherley Sara C. , Champsi Sabrina , Aleali Nadia , Shakeri Dorsa , El-Zahed Maya , Akbarian Nicky , Nejad-Mansouri Mehran , Bavani Parinaz Z. , Liggins Richard L. , Chan Owen , Riddell Michael C. 
Published: February 2024

Background: Elevated levels of somatostatin blunt glucagon counterregulation during hypoglycemia in type 1 diabetes (T1D) and this can be improved using somatostatin receptor 2 (SSTR2) antagonists. Hypoglycemia also occurs in late-stage type 2 diabetes (T2D), particularly when insulin therapy is initiated, but the utility of SSTR2 antagonists in ameliorating hypoglycemia in this disease state is unknown. We examined the efficacy of a single-dose of SSTR2 antagonists in a rodent model of T2D.Methods: High-fat fed (HFF), low dose streptozotocin (STZ, 35 mg/kg)-induced T2D and HFF only, nondiabetic (controls-no STZ) rats were treated with the SSTR2 antagonists ZT-01/PRL-2903 or vehicle (n = 9–11/group) 60 min before an insulin tolerance test (ITT; 2–12 U/kg insulin aspart) or an oral glucose tolerance test (OGTT; 2 g/kg glucose via oral gavage) on separate days.Results: This rodent model of T2D is characterized by higher baseline glucose and HbA1c levels relative to HFF controls. T2D rats also had lower c-peptide levels at baseline and a blunted glucagon counterregulatory response to hypoglycemia when subjected to the ITT. SSTR2 antagonists increased the glucagon response and reduced incidence of hypoglycemia, which was more pronounced with ZT-01 than PRL-2903. ZT-01 treatment in the T2D rats increased glucagon levels above the control response within 60 min of dosing, and values remained elevated during the ITT (glucagon Cmax: 156 ± 50 vs. 77 ± 46 pg/mL, p &lt; 0.01). Hypoglycemia incidence was attenuated with ZT-01 vs. controls (63% vs. 100%) and average time to hypoglycemia onset was also delayed (103.1 ± 24.6 vs. 66.1 ± 23.6 min, p &lt; 0.05). ZT-01 administration at the OGTT onset increased the glucagon response without exacerbating hyperglycemia (2877 ± 806 vs. 2982 ± 781), potentially due to the corresponding increase in c-peptide levels (6251 ± 5463 vs. 14008 ± 5495, p = 0.013).Conclusion: Treatment with SSTR2 antagonists increases glucagon responses in a rat model of T2D and results in less hypoglycemia exposure. Future studies are required to determine the best dosing periods for chronic SSTR2 antagonism treatment in T2D.
Regulation of tissue growth in plants – A mathematical modeling study on shade avoidance response in Arabidopsis hypocotyls
Authors: Favre Patrick , van Schaik Evert , Schorderet Martine , Yerly Florence , Reinhardt Didier 
Published: February 2024

IntroductionPlant growth is a plastic phenomenon controlled both by endogenous genetic programs and by environmental cues. The embryonic stem, the hypocotyl, is an ideal model system for the quantitative study of growth due to its relatively simple geometry and cellular organization, and to its essentially unidirectional growth pattern. The hypocotyl of Arabidopsis thaliana has been studied particularly well at the molecular-genetic level and at the cellular level, and it is the model of choice for analysis of the shade avoidance syndrome (SAS), a growth reaction that allows plants to compete with neighboring plants for light. During SAS, hypocotyl growth is controlled primarily by the growth hormone auxin, which stimulates cell expansion without the involvement of cell division.MethodsWe assessed hypocotyl growth at cellular resolution in Arabidopsis mutants defective in auxin transport and biosynthesis and we designed a mathematical auxin transport model based on known polar and non-polar auxin transporters (ABCB1, ABCB19, and PINs) and on factors that control auxin homeostasis in the hypocotyl. In addition, we introduced into the model biophysical properties of the cell types based on precise cell wall measurements.Results and DiscussionOur model can generate the observed cellular growth patterns based on auxin distribution along the hypocotyl resulting from production in the cotyledons, transport along the hypocotyl, and general turnover of auxin. These principles, which resemble the features of mathematical models of animal morphogen gradients, allow to generate robust shallow auxin gradients as they are expected to exist in tissues that exhibit quantitative auxin-driven tissue growth, as opposed to the sharp auxin maxima generated by patterning mechanisms in plant development.
Meta-analysis of exercise intervention on health behaviors in middle-aged and older adults
Authors: Liu Min , Mei Dong-hui , Zhang Ya-lu , Kang Ning , Wang Dong-min , Chen Gong 
Published: February 2024

ObjectiveTo systematically review and analyze the effects of exercise interventions on health behavior among middle-aged and older adults.MethodsA Meta-analysis was performed using NoteExpress software to screen randomized controlled trials (RCTs) published between January 1, 2000, and March 31, 2023, which were identified through databases including CNKI, Web of Science, Pubmed, and 6 more sources, based on predefined literature inclusion criteria. Following the quality assessment, we conducted both the overall and subgroup Meta-analyses of the exercise intervention moderator variables using Review Manager 5.4.1 software, encompassing data among the 18 RCTs. The effect size was measured as the standardized mean difference within its corresponding 95% confidence interval. Heterogeneity was assessed using the I2 metrics.Results(1) The overall results indicate a significant impact of exercise intervention on health behaviors, characterized by a large effect size [SMD = 1.02 &gt; 0.8, 95% CI (0.73, 1.32), P &lt; 0.001]. (2) The highest degree of heterogeneity in the relationship between exercise interventions and health behaviors was associated with the duration of one exercise session (I2= 71.2%), which was the most influential moderator variable. (3) The aerobic and resistance intervention in group exercise lasting 30–60 min per time a day, 6–7 times per week over a period of 8–12 weeks demonstrated the most substantial effect size.Conclusion(1) The exercise intervention significantly promotes the health behavior in middle-aged and older adults, emphasizing the importance of carefully considering the duration of individual exercise sessions when designing and implementing exercise intervention. (2) Considering the accessibility for middle-aged and older adults, the optimal exercise intervention should include the means of group practice, the types of aerobic and resistance exercise, with a duration of 30–60 min per time a day, beginning with a lower weekly frequency and gradually increase to 6–7 times per week, and lasting for 8 to 12 weeks.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024506750.
Accuracy of correction of a hexapod frame, patient-specific osteotomy and reduction guides, and hinged circular external fixation in a 3D-printed canine antebrachial deformity model
Authors: Burton Neil J. , Oxley Bill 
Published: February 2024

ObjectivesThis study aimed to objectively define whether human hexapod fixation (Maxframe), with or without the use of 3D-printed positioning guides, can correct a canine antebrachial deformity with greater accuracy than the clinically established techniques of 3D patient-specific osteotomy and reduction guides (3D-PSORG) or hinged circular external skeletal fixation (CESF).MethodsCT of a canine antebrachium was manipulated to induce distal radial deformity of the valgus, external torsion, and procurvatum, each of magnitude 20o. Five experiments were performed to correct the deformity via a distal radial and ulna opening osteotomy using: (1) A 3D-PSORG with the application of a locking plate, (2) hinged CESF, (3) Maxframe standard protocol, (4) Maxframe applied with patient-specific positioning guides (PSPGs), and (5) Maxframe with frame adjustment calculated from post-application CT. Following correction, all constructs were optically scanned, and objective measurement of the correction achieved was performed.ResultsNo construct returned the distal bone segment to its preoperative position in all planes. Translational malalignment in the sagittal plane had the highest magnitude of error for all constructs, with the Maxframe standard protocol showing the greatest error. Maxframe (PSPGs) showed the minimum error of all constructs in the frontal and sagittal planes.Clinical significanceIn this 3D-printed model of antebrachial deformity correction, the hexapod frame with the use of PSPGs achieved better accuracy than 3D-PSORG and hinged CESF and may be a technique of future interest and development in the management of canine antebrachial limb deformity.
Magnetic resonance imaging prognostic factors for survival and relapse in dogs with meningoencephalitis of unknown origin
Authors: Gonçalves Rita , De Decker Steven , Walmsley Gemma , Maddox Thomas W. 
Published: February 2024

IntroductionCanine meningoencephalitis of unknown origin (MUO) is a debilitating disease associated with high mortality. The prognostic value of magnetic resonance imaging (MRI) findings for predicting survival at 12 months and long-term relapse remains uncertain.MethodsThis was a retrospective cohort study evaluating the prognostic value of different MRI variables using multivariable logistic regression and Cox proportional hazards analysis.ResultsIn total, 138 dogs were presumptively diagnosed with MUO. The most common location for lesions identified on MRI were the white matter tracts of the corona radiata and corpus callosum, followed by the frontal, sensorimotor and temporal cortices. Lower T2 lesion load (p = 0.006, OR = 0.942, CI = 0.902–0.983) was associated with longer survival and higher T1 post-contrast lesion load (p = 0.023, OR = 1.162, CI = 1.021–1.322) was associated with relapse.DiscussionThis study has identified prognostic factors that may help identify dogs at higher risk of death and relapse and therefore guide treatment recommendations.
Effects of Tetrabasic Zinc Chloride on the Diarrhea Rate, Intestinal Morphology, Immune Indices and Microflora of Weaned Piglets
Authors: Peng Shuyu , Zhang Nan , Zhang Tuan , Zhang Yu , Dong Shuang , Wang Huiyun , Xu Cong , Wang Chunlin 
Published: February 2024

This study was aimed to investigate the effects of different dietary zinc sources on the diarrhea rate, intestinal morphology, immune indexes and intestinal microbial composition of weaned piglets. A total of 240 weaned piglets (Duroc × Landrace × Yorkshire), at the age of 21 days, were randomly assigned to five dietary treatments for a four-week feeding trial to determine the effects of different amounts of tetrabasic zinc chloride (TBZC) supplementation on intestinal morphology, intestinal immune indices and intestinal microflora in weaned piglets, compared with the pharmacological dose of ZnO. The dietary treatments included a negative control (CON), (T1) ZnO (ZnO, 1500 mg/kg), (T2) tetrabasic zinc chloride (TBZC, 800 mg/kg), (T3) tetrabasic zinc chloride (TBZC, 1000 mg/kg), and (T4) tetrabasic zinc chloride (TBZC, 1200 mg/kg). Each treatment comprised six replicate pens, with eight pigs (four barrows and four gilts) per pen. Dietary TBZC of 1200 mg/kg improved the duodenum villus height, jejunum villus height and crypt depth of ileum, and increased the ratio of villus height to crypt depth of ileum (p < 0.05). The dietary supplementation of TBZC at a dosage of 1200 mg/kg has the potential to increase the levels of immunoglobulin G (IgG) and immunoglobulin A (IgA) in the duodenal mucosa. Furthermore, it shows a significant increase in the levels of immunoglobulin A (IgA) in the ileum. Compared with CON, TBZC significantly (p < 0.05) decreased pH values of stomach contents. It also increased the number of Firmicutes in intestinal contents. Compared with CON, the abundance of Firmicutes in jejunum contents of other treatments was significantly improved (p < 0.05), while the abundance of Proteobacteria in ileum contents of high-zinc treatments (T2 and T5) was decreased (p < 0.05). In conclusion, dietary TBZC of 1200 mg/kg improved the digestibility of crude protein in weaned piglets, altered the intestinal morphology of piglets, changed the intestinal microflora of piglets, reduced the diarrhea rate, and significantly improved the development of the small intestine of weaned piglets, and its regulation mechanism on intestinal tract needs further study. In summary, TBZC is likely to be an effective substitute source for the pharmacological dose of ZnO to control diarrhea in weaned piglets.
Initial eGFR Changes Predict Response to Tolvaptan in ADPKD
Authors: Mochizuki Toshio , Matsukawa Miyuki , Tanaka Toshiki , Jiang Huan 
Published: February 2024

Key Points

This post hoc analysis of the Tolvaptan Efficacy and Safety in Management of Autosomal Dominant Polycystic Kidney Disease and Its Outcomes 3:4 study investigated the long-term predictive potential of initial changes in eGFR.Initial eGFR change from baseline to week 3 proved to be a significant and independent indicator of the long-term effects of tolvaptan.No correlation was found between the initial change in eGFR and the annual rate of percent growth in total kidney volume.

Background
Tolvaptan, the only pharmaceutical treatment available for autosomal dominant polycystic kidney disease (ADPKD), reduced the rates of total kidney volume (TKV) increase and kidney function decline in patients with ADPKD in the global phase 3 Tolvaptan Efficacy and Safety in Management of Autosomal Dominant Polycystic Kidney Disease and Its Outcomes (TEMPO) 3:4 study. Since tolvaptan initiation is associated with an initial decline in the eGFR, this post hoc analysis of the TEMPO 3:4 study investigated whether initial changes in eGFR from baseline to week 3 after tolvaptan administration can predict its longer-term effects on eGFR and TKV in patients with ADPKD.

Methods
eGFR was estimated using the CKD Epidemiology Collaboration equation at baseline and up to month 36. TKV was estimated using standardized kidney magnetic resonance imaging at baseline and after 12, 24, and 36 months of tolvaptan treatment. The effect of tolvaptan on kidney function and kidney volume was evaluated by measuring changes in eGFR from week 3 and TKV from baseline up to 36 months. All 961 patients randomized to receive tolvaptan in TEMPO 3:4 were included in this analysis.

Results
Initial change in eGFR from baseline to week 3 was a significant and independent predictor of the mean rate of change in eGFR per year. By contrast, there was no association between initial change in eGFR and the rate of percent growth in TKV per year.

Conclusions
Changes in eGFR after 3 weeks of treatment are likely due to the pharmacologic effect of tolvaptan, and these initial changes are predictive of the long-term effects of tolvaptan treatment.
PENGARUH PRILAKU DAN LINGKUNGAN KERJA TERHADAP KINERJA GENERASI Z PADA PERUSAHAAN BLUE ECONOMY DI PROBOLINGGO
Authors: Bryant Arya Adirajasa  , Noviaty Kresna Darmasetiawan  
Published: February 2024

Abstract. The type of research used is quantitative by examining organizational performance by looking at employee job performance. This research is clause research and will use random sampling techniques. Respondents are company employees with a total of 200 respondents and questionnaires will be distributed. It was found that employee performance is an important factor in organizational performance which will have an impact on blue economy companies in Probolinggo. This research will provide an understanding of organizational performance on the basis of organizational performance (OP) which includes several influences regarding work attitude, job satisfaction and work environment which have an impact on job performance in generation Z in blue economy companies.

Abstrak. Tipe penelitian yang digunakan adalah kuantitaif dengan menguji performa organisasi dengan melihat job performance pada karyawan. Penelitian merupakan clausal research ini akan menggunakan teknik random sampling. Responden merupakan karyawan perusahaan dengan jumlah 200 responden dan akan dilakukan penyebaran kuisioner. Ditemukan bahwa performance karyawan menjadi fakor penting dalam organizational performance yang akan berdampak pada blue economy company di Probolinggo. Penelitian ini akan memberikan pemahaman mengenai organizational performance dengan dasar organizational performance (OP) yang mencakup beberapa pengaruh mengenai work attitude, job satisfaction dan work environment yang berdampak pada job performance pada generasi Z di blue economy company.
THE INFLUENCE OF PARENTAL SUPPORT DIMENSIONS IN SHAPING THE INTENTION TO CONTINUE THE FAMILY BUSINESS DAUGHTER SUCCESSORS WITH SELF-EFFICACY AS A MEDIATOR
Authors: Nisa Puspayani , Padmalia Metta 
Published: February 2024

Abstract. The intention to continue the family business can be influenced in particular by parents. This study discusses the influence of parental support dimensions including Instrumental Assistance (IA) and Career-related modeling (CRM) in forming the intention to continue a family business that focuses on female successors with self-efficacy as mediation. Through this research, it is expected to be able to provide the latest views, especially girls, when in the family business. The sampling technique used was a purposive sampling technique on 195 girls aged at least 18 years who had contributed to the family business. This research was tested using SmartPLS 3.0 and JASP software. Through this study, the findings of this study indicate that both Instrumental Assistance (IA) and self-efficacy play significant roles in shaping a daughter's inclination to achieve success within the context of a family business. Unlike the previous Career-Related Modeling (CRM) based on specific studies indicate that self-efficacy does not serve as a mediating factor in the relationship between Career-Related Modelling and a daughter's intention to succeed in the family business.

Abstrak. Tujuan untuk melanjutkan bisnis keluarga dapat dipengaruhi khususnya oleh orang tua. Penelitian ini membahas tentang pengaruh dimensi dukungan orang tua meliputi Instrumental Assistance (IA) dan Career-related modeling (CRM) dalam membentuk niat untuk melanjutkan bisnis keluarga yang fokus pada penerus perempuan dengan efikasi diri sebagai mediasinya. Melalui penelitian ini diharapkan mampu memberikan pandangan terkini khususnya anak perempuan ketika terjun dalam bisnis keluarga. Teknik pengambilan sampel yang digunakan adalah teknik purposive sampling terhadap 195 anak perempuan berusia minimal 18 tahun yang pernah berkontribusi pada bisnis keluarga. Penelitian ini diuji dengan menggunakan software SmartPLS 3.0 dan JASP. Melalui penelitian ini, temuan penelitian ini menunjukkan bahwa baik Instrumental Assistance (IA) maupun self-efisiensi berperan penting dalam membentuk kecenderungan anak perempuan untuk mencapai kesuksesan dalam konteks bisnis keluarga. Berbeda dengan Career-Related Modeling (CRM) sebelumnya, berdasarkan penelitian tertentu menunjukkan bahwa self- efikasi tidak menjadi faktor mediasi dalam hubungan antara Career-Related Modeling dan niat anak perempuan untuk sukses dalam bisnis keluarga
PENGARUH ORGANIZATION COMMITMENT TERHADAP ORGANIZATIONAL CITIZENSHIP BEHAVIOR DENGAN PERSON-ORGANIZATION FIT SEBAGAI MODERATOR PADA KARYAWAN OAKWOOD HOTEL & RESIDENCES SURABAYA
Authors: Ecinsia Emi , Nugroho J.L. Eko , Widjaja M.E. Lanny Kusuma 
Published: February 2024

Penelitian ini bertujuan untuk mengetahui pengaruh organization commitment terhadap organizational citizenship behavior dengan person-organization fit sebagai moderator pada karyawan Oakwood Hotel & Residences Surabaya. Pada penelitian ini akan melihat sejauh mana peran perceived organizational support, employee development, dan organization commitment dalam memengaruhi organizational citizenship behavior karyawan Oakwood Hotel & Residences Surabaya. Penelitian ini menggunakan pendekatan kuantitatif dengan menggunakan analisis Structural Equation Modelling-Partial Least Square (SEM-PLS) menggunakan software SmartPLS dengan jumlah sampel sejumlah 115 responden. Penelitian ini memperlihatkan hasil bahwa perceived organizational support, employee development,dan organization commitment berpengaruh positif dan signifikan terhadap organizational citizenship behavior serta person-organization fit memoderasi hubungan antara organization commitment dengan organizational citizenship behavior.