bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune heparin‐induced thrombocytopenia resulting from preceding exposure to heparin catheter flushes
Authors: Muslimani Alaa A. , Ricaurte Basma , Daw Hamed A. 
Published: December 2006

AbstractImmune heparin‐induced thrombocytopenia (HIT) is a life‐threatening adverse effect of heparin. It can result from any type of heparin exposure and by any route of administration; however only a few cases are reported after exposures to small quantities of heparin from catheter flushes. The major clinical problem associated with HIT is thrombosis. Early detection and institution of alternative, non‐heparin anticoagulation are important. We report a patient with HIT associated with use of therapeutic‐dose unfractionated heparin in whom immune sensitization to heparin was triggered by two 500‐unit exposure to UFH associated with intravascular catheter flushing for antineoplastic chemotherapy in a patient with colon adenocarcinoma. Am. J. Hematol. 82: 2007. © 2006 Wiley‐Liss, Inc.
Diels–Alder Reactions of Novel (1′‐Arylallylidene)cyclopropanes with Heterodienophiles
Authors: Zhao Ligang , Yucel Baris , Scheurich René Peter , Frank Daniel , de Meijere Armin 
Published: December 2006

AbstractPalladium‐catalyzed cross‐coupling of various aryl iodides with bicyclopropylidene provided isolable (1′‐arylallylidene)cyclopropanes, which reacted with a number of carbonyl compounds in the presence of Eu(fod)3 under high pressure to furnish oxaspiro[2.5]octene derivatives in moderate to good yields (22–69 %). The reactions of the allylidenecyclopropanes with two azo compounds as dienophiles afforded diazaspiro[2.5]octenes in high yields (82 and 99 %) even at ambient pressure. When treated with nitrosobenzene, two of the allylidenecyclopropanes gave the Diels–Alder adducts in up to 83 and 40 % yield. 2,5‐Diiodo‐p‐xylene coupled twice with bicyclopropylidene, and the product underwent a twofold Diels–Alder reaction with nitrosobenzene to produce the bis(spirocyclopropaneoxazine) derivative in 88 % yield. This overall transformation can be brought about in a one‐pot, two‐step operation by addition of the nitrosoarene to the reaction mixture immediately after formation of the allylidenecyclopropanes to furnish various 5‐oxa‐4‐azaspiro[2.5]oct‐7‐ene derivatives in 22–77 % yield. The coupling of methyl bicyclopropylidenecarboxylate with 2,6‐dimethylphenyl iodide produced a mixture of very stable regioisomeric allylidenecyclopropane derivatives in 90 % yield. The reaction of this mixture with N‐phenyltriazolinedione gave a corresponding mixture of the spirocyclopropanated heterobicycles in 61 % yield.
Pathologic findings and clinical outcome of patients undergoing retroperitoneal lymph node dissection after multiple chemotherapy regimens for metastatic testicular germ cell tumors
Authors: Eggener Scott E. , Carver Brett S. , Loeb Stacy , Kondagunta G. Varuni , Bosl George J. , Sheinfeld Joel 
Published: December 2006

AbstractBACKGROUND.Postchemotherapy surgery is an essential component in the management of patients with metastatic germ cell tumors (GCT). The authors assessed their institutional experience of retroperitoneal lymph node dissection (RPLND) after multiple chemotherapy regimens for advanced GCT.METHODS.By analyzing the institutional prospective surgical database from 1989 to 2004, 71 patients were identified who underwent RPLND after multiple chemotherapy regimens. Clinicopathologic and treatment trends were characterized, and predictors of disease‐specific survival (DSS) were evaluated.RESULTS.The histologic findings at RPLND were fibrosis in 36 men (51%), GCT in 20 men (28%), and teratoma in 15 men (21%). Patients who underwent RPLND from 1989 to 1998 (n = 47), compared with patients who underwent RPLND from 1999 to 2004 (n = 24) were more likely to have GCT (36% vs 13%; P = .04). Patients who received taxane‐containing chemotherapy regimens as salvage therapy had lower rates of GCT at RPLND (14% vs 42%; P = .01), higher rates of fibrosis (63% vs 39%; P = .04), and similar rates of teratoma (31% vs 33%; P = .9). The 5‐ and 10‐year DSS rates were 74% (95% confidence interval [95% CI], 62–86%) and 70% (95% CI, 56–84%), respectively. Five‐year DSS based on worst histology of RPLND and extraretroperitoneal specimens was 87% (95% CI, 75–99%) for fibrosis, 87% for teratoma (95% CI, 63–100%), and 47% for GCT (95% CI, 23–71%; P = .004). On multivariable analysis, retroperitoneal mass ≥5 cm and GCT were predictors of worse DSS (P = .03 and P = .005, respectively).CONCLUSIONS.Taxane‐based salvage chemotherapeutic regimens appear to have decreased the rate of GCT at RPLND. The current data support RPLND in select patients after salvage chemotherapy, because a considerable proportion has teratoma or GCT, and the 10‐year DSS rate after resection is 70%. Cancer 2007;109:528–535. © 2006 American Cancer Society.
Stabilized Lipid Membrane Based Biosensors with Incorporated Enzyme for Repetitive Uses
Authors: Nikolelis Dimitrios P. , Raftopoulou Garyfallia , Nikoleli Georgia‐Paraskevi , Simantiraki Maria 
Published: December 2006

AbstractThis work reports a technique for the stabilization of lipid membrane based biosensors with incorporated enzyme that retains its activity for repetitive uses. Microporous filters composed of glass fibers were used as supports for the stabilization of these sensors. The lipid film is formed on the filter by polymerization using UV (ultraviolet) radiation prior its use. Methacrylic acid was the functional monomer, ethylene glycol dimethacrylate was the crosslinker and 2,2′‐azobis‐(2‐methylpropionitrile) was the initiator. The enzyme (acetylcholinesterase) is incorporated within this mixture prior to polymerization. The polymerization process takes place by using UV irradiation instead of heating at 60 °C the lipid mixture because this temperature might denature the enzyme. This method for preparation of stabilized lipid membranes was investigated using Raman spectroscopy. The results have indicated that the kinetics of polymerization are completed within 4 hours. The retain in activity of the enzyme was studied using electrochemical experiments which have shown that this mild technique of polymerization can now be used to incorporate a protein in these lipid membranes without loss of their activity. This will allow the practical use of the techniques for chemical sensing based on lipid membranes based biosensors and commercialization of these devices.
Influence of polymorphisms at loci encoding DNA repair proteins on cancer susceptibility and G2chromosomal radiosensitivity
Authors: Wilding Craig S. , Curwen Gillian B. , Tawn E. Janet , Sheng Xiaohua , Winther Jeanette F. , Chakraborty Ranajit , Boice John D. 
Published: December 2006

AbstractSixteen candidate polymorphisms (13 SNPs and 3 microsatellites) in nine genes from four DNA repair pathways were examined in 83 subjects, comprising 23 survivors of childhood cancer, their 23 partners, and 37 offspring, all of whom had previously been studied for G2chromosomal radiosensitivity. Genotype at the Asp148Glu SNP site in theAPEXgene of the base excision repair (BER) pathway was associated with childhood cancer in survivors (P= 0.001, significant even after multiple test adjustment), due to the enhanced frequency of theAPEXAsp148 allele among survivors in comparison to that of their partners. Analysis of variance (ANOVA) of G2radiosensitivity in the pooled sample, as well as family‐based association test (FBAT) of the family‐wise data, showed sporadic suggestions of associations between G2radiosensitivity and polymorphisms at two sites (the Thr241Met SNP site in theXRCC3gene of the homologous recombinational pathway by ANOVA, and the Ser326Cys site in thehOGG1gene of the BER pathway by FBAT analysis), but neither of these remained significant after multiple‐test adjustment. This pilot study provides an intriguing indication that DNA repair gene polymorphisms may underlie cancer susceptibility and variation in radiosensitivity. Environ. Mol. Mutagen., 2007. © 2006 Wiley‐Liss, Inc.
Chemical composition of seed essential oils from Algerian Nigella sativa extracted by microwave and hydrodistillation
Authors: Benkaci–Ali Farid , Baaliouamer Aoumeur , Meklati Brahim Y. , Chemat Farid 
Published: December 2006

AbstractExtraction of essential oil from Nigella sativa seed collected at two locations in the Sahara desert, Timimoun (T) and Adrar (A), has been conducted by hydrodistillation (HD) and a microwave distillation process (MD). The composition of the volatile oil was investigated by capillary gas chromatography and gas chromatography–mass spectrometry. Using the hydrodistillation, active compounds such as p‐cymene (8.9 and 7.2% in T and A, respectively), 4‐terpineol (0.6–8.9%), thymohydroquinone (6.1–12.2%), thymoquinone (1.6–21.8%), carvacrol (12.9–12.9%), carvone (4.4–0.3%) and thymol (1.5–0.7%) were the major components, representing more than 36% (T) and 64% (A) of the oils. For the microwave distillation, p‐cymene (28.1% and 32.0% in T and A, respectively), 4‐terpineol (3.4–2.0%), thymohydroquinone (0.7–1.1%), thymoquinone (10.8–24.6%), carvacrol (3.0–6.0%), and thymol (0.3–0.3%) represent respectively 46.1% and 66.0% of the T and A oils. Copyright © 2006 John Wiley & Sons, Ltd.
Composition and antioxidant activity of the essential oil of Teucrium royleanum Wall. ex Benth growing in Pakistan
Authors: Saroglou V. , Arfan M. , Shabir A. , Hadjipavlou‐Litina D. , Skaltsa H. 
Published: December 2006

AbstractThe chemical composition of the essential oil obtained from the leaves of Teucrium royleanum, growing in Pakistan, was investigated by GC–MS analyses. Kováts indices, mass spectra and standard compounds were used to identify a total of 46 individual compounds. Sesquiterpene hydrocarbons formed the main portion (42.2%) of the oil, among which β‐santalene and cis‐α‐bisabolene were the predominant compounds. The potential antioxidant activity was also investigated and the sample tested was found to interact slightly with the stable free radical DPPH in a time‐dependent manner. Copyright © 2006 John Wiley & Sons, Ltd.
An intervention to improve depression care in older adults with COPD
Authors: Sirey Jo Anne , Raue Patrick J. , Alexopoulos George S. 
Published: December 2006

AbstractObjectiveTo describe an intervention for older persons with Major Depressive Disorder (MDD) and Chronic Obstructive Pulmonary Disease (COPD) to improve adherence to psychiatric, medical, and rehabilitation recommendations. The intervention supplements antidepressant therapy for depression with an individualized care manager who targets psychological barriers that interfere with treatment participation.MethodDescription of intervention development, training, and barriers to care, and illustration with case example.ResultsDepression and its associated lack of motivation, helplessness, and lack of energy can obstruct active participation in rehabilitation exercises recommended for COPD. Additionally, depressed older adults perceive the benefits of depression treatment; however they also fear side effects, addiction to antidepressants and have concerns about stigma. The intervention elucidates individual attitudes and beliefs that may become barriers. The care manager works with the older adult to address the barriers and improve treatment participation.ConclusionAugmentation of traditional pharmacotherapy for depression with a care manager can improve adherence to both depression and COPD treatment. This improved adherence may lessen the physical, psychological, and functional costs of both diseases. Copyright © 2006 John Wiley & Sons, Ltd.
Dementia care mapping as a research tool
Authors: Sloane Philip D. , Brooker Dawn , Cohen Lauren , Douglass Carolinda , Edelman Perry , Fulton Bradley R. , Jarrott Shannon , Kasayka Roseann , Kuhn Daniel , Preisser John S. , Williams Christianna S. , Zimmerman Sheryl 
Published: December 2006

AbstractIntroductionDementia Care Mapping (DCM) was originally developed as a clinical tool but has attracted interest as a potential observational measure of quality of life (QOL) and well‐being of long‐term care residents with dementia. DCM coding involves continuous observation over a 6‐h period, with observers recording a Behavior Category Code (BCC, a recording of activity/interaction) and a Well/Ill Being (WIB) score at 5 min intervals.MethodDescriptive data from several different research teams on the distribution and psychometric properties of DCM data were compiled and summarized.ResultsIssues and problems identified include: complex scoring algorithms, inter‐rater reliability of the BCCs, limited variability of WIB values, associations between resident characteristics and DCM assessments, rater time burden, and comparability of results across study settings.ConclusionsDespite the identified limitations, DCM has promise as a research measure, as it may come closer to rating QOL from the perspective of persons with dementia than other available measures. Its utility will depend on the manner in which it is applied and an appreciation of the measure's strength and limitations. Possible changes that might improve the reliability, validity, and practicality of DCM as a research tool include coding the predominant event (rather than the ‘best’ event), shortening the observation period, and adding ‘0’ as a neutral WIB coding option. Copyright © 2006 John Wiley & Sons, Ltd.
Monte Carlo probabilistic sensitivity analysis for patient level simulation models: efficient estimation of mean and variance using ANOVA
Authors: O'Hagan Anthony , Stevenson Matt , Madan Jason 
Published: December 2006

AbstractProbabilistic sensitivity analysis (PSA) is required to account for uncertainty in cost‐effectiveness calculations arising from health economic models. The simplest way to perform PSA in practice is by Monte Carlo methods, which involves running the model many times using randomly sampled values of the model inputs. However, this can be impractical when the economic model takes appreciable amounts of time to run. This situation arises, in particular, for patient‐level simulation models (also known as micro‐simulation or individual‐level simulation models), where a single run of the model simulates the health care of many thousands of individual patients. The large number of patients required in each run to achieve accurate estimation of cost‐effectiveness means that only a relatively small number of runs is possible. For this reason, it is often said that PSA is not practical for patient‐level models.We develop a way to reduce the computational burden of Monte Carlo PSA for patient‐level models, based on the algebra of analysis of variance. Methods are presented to estimate the mean and variance of the model output, with formulae for determining optimal sample sizes. The methods are simple to apply and will typically reduce the computational demand very substantially. Copyright © 2006 John Wiley & Sons, Ltd.
Expression of somatic DNA repair genes in human testes
Authors: Galetzka Danuta , Weis Eva , Kohlschmidt Nicolai , Bitz Oliver , Stein Raimund , Haaf Thomas 
Published: December 2006

AbstractMeiosis is the key process for recombination and reduction of the diploid chromosome set to a haploid one. Many genes that have been found in yeast or mouse models to play a role in meiosis are also important for the repair of DNA damage in somatic cells. To study the DNA repair gene transcriptome during male germ cell development, we have developed a specialized cDNA microarray with 181 human genes which are involved in different somatic DNA repair pathways and/or cell cycle control and 45 control house‐keeping genes. This DNA repair gene chip was used to quantify the mRNA expression levels in three human testes samples versus a fibroblast RNA pool. Two hundred twenty genes on the chip (including house‐keeping genes) showed detectable expression levels in adult testes. Sixty‐four DNA repair‐ and cell cycle‐associated genes showed higher expression levels in testicular cells than in mitotically dividing fibroblasts and, therefore, are likely to be implicated in meiosis. The microarray results of 17 genes with increased expression levels were validated with reverse Northern blots or real‐time quantitative RT PCR. Systematic analyses of the meiotic DNA repair gene transcriptome may provide new insights into the genetics of male (in)fertility. J. Cell. Biochem. 100: 1232–1239, 2007. © 2006 Wiley‐Liss, Inc.
Tumor–lymphatic interactions in an activated stromal microenvironment
Authors: Wong Sunny Y. , Hynes Richard O. 
Published: December 2006

AbstractMetastasis to lymph nodes is a common feature of many human tumors and may facilitate dissemination to other parts of the body. Peritumoral lymphatics, which are located at the periphery of a primary tumor, appear to be anything but peripheral for metastasis, as recent studies have highlighted their critical role in disseminating tumor cells to local lymph nodes. The metastatic process, including lymphangiogenesis, is likely governed by a complex series of interactions among tumor cells, endothelial cells, and non‐endothelial stromal components. Therefore, a detailed understanding of the biology of the tumor microenvironment, particularly as it pertains to peritumoral lymphatics near the tumor–stromal junction, may someday translate into clinical approaches that target metastasis at the invasive front. J. Cell. Biochem. 101: 840–850, 2007. © 2006 Wiley‐Liss, Inc.
Comparison of the medium molecular weight venom fractions from five species of common social wasps by MALDI‐TOF spectra profiling
Authors: Turillazzi Stefano , Bruschini Claudia , Lambardi Duccio , Francese Simona , Spadolini Igino , Mastrobuoni Guido 
Published: December 2006

AbstractThe average spectral profiles and the exact mass weight (MW) of biomolecules present in the medium fraction (from 900 to 3000 Da) of the venom of five social wasps (three European and one North American Polistes and the European hornet Vespa crabro) were determined by matrix assisted laser desorption ionization time of flight (MALDI‐TOF) MS. Data were obtained analyzing the venom of single specimens (N = 46) and elaborated with the ClinProTools 2.0 (CPT) software to search for differences among the five species examined. Interesting differences in the spectral profiles were found, allowing the discrimination of venoms belonging to the different species, and their possible use as a quality control method in venom immunotherapy (VIT) for allergic patients. Copyright © 2006 John Wiley & Sons, Ltd.
Chronic haloperidol treatment results in a decrease in the expression of myelin/oligodendrocyte‐related genes in the mouse brain
Authors: Narayan Sujatha , Kass Kristi E. , Thomas Elizabeth A. 
Published: December 2006

AbstractSchizophrenia is a complex psychiatric illness that manifests as a combination of positive symptoms, negative symptoms, and cognitive deficits. Antipsychotic drugs, such as haloperidol, attenuate dopamine receptor signaling in neurons and constitute the frontline treatment for the positive symptoms of schizophrenia. However, haloperidol treatment has also been reported to exacerbate preexisting negative symptoms/cognitive deficits and the severity of these deficits has been correlated with white matter pathology in schizophrenia. Indeed, several studies implicate oligodendrocyte function in the pathophysiology of schizophrenia, but it is unknown whether these effects are related to drug treatment. It is well established that haloperidol alters gene expression in neurons. However, its effect on oligodendrocytes is unknown. In this study, we investigate the effects of chronic haloperidol treatment on the expression of eight genes known to play critical roles in myelin/oligodendrocyte function. We treated male mice with haloperidol (2 mg/kg/day) for 30 days and measured gene expression changes by using in situ hybridization analysis and quantitative densitometry. Haloperidol caused a decrease in the expression of these genes in several white matter regions of the mouse CNS. In contrast, clozapine (10 mg/kg/day) had no effect on the expression of a subset of these genes. This has important implications for both disease pathology and the consideration of treatment options for patients. © 2006 Wiley‐Liss, Inc.
Study of UV‐shielding properties of novel porous hollow silica nanoparticle carriers for avermectin
Authors: Li Zhu‐Zhu , Chen Jian‐Feng , Liu Fan , Liu An‐Qi , Wang Qing , Sun Hai‐Yan , Wen Li‐Xiong 
Published: December 2006

AbstractThe shielding protection given by self‐prepared porous hollow silica nanoparticles (PHSN) to pesticides from degradation by UV light was investigated using avermectin as a model pesticide. It was demonstrated that PHSN carriers with a shell thickness of ∼15 nm and a pore diameter of 4–5 nm have an encapsulation capacity of 625 g kg−1 for avermectin using a supercritical fluid loading method. PHSN carriers exhibited remarkable UV‐shielding properties for avermectin. This was affected by the intensity of UV light, the pH and the temperature of the release medium. Rises in UV intensity, pH and/or temperature reduced the UV protection of PHSN for avermectin. In addition, avermectin loaded into the inner core of the PHSN carriers was released slowly into the release medium for about 30 days following a typical sustained‐release pattern. It thus appears that PHSN carriers have a promising future in applications requiring sustained pesticide release. Copyright © 2007 Society of Chemical Industry
Effect of substituents at the 5‐position of the pyridine ring of imidacloprid on insecticidal activity against Periplaneta americana
Authors: Kagabu Shinzo , Murase Yuko , Imai Rie , Ito Nakako , Nishimura Keiichiro 
Published: December 2006

AbstractThe insecticidal activities of imidacloprid derivatives with a wide range of substituents at the 5‐position on the pyridine ring against American cockroaches, Periplaneta americana (L.), were measured by injection with and without synergists propyl 2‐propynyl phenylphosphonate and piperonyl butoxide. The log(1/MLD) value (MLD = minimal lethal dose in mol) without synergists was 7.96 for the methyl derivative, and the values were lower for other derivatives. Synergists enhanced the potencies of all the compounds tested. Considering these compounds and those with other substituents at this position, the region for maximum activity was predicted to be in the conjunction of the pyridyl 6‐chlorine atom with a lipophilic small group in the 5‐position. Copyright © 2007 Society of Chemical Industry
Theoretical study of the gas‐phase ion pairs SN2 reactions of LiX with CH3SY (X, Y = F, Cl, Br, I)
Authors: Gai Jing‐Gang , Ren Yi 
Published: December 2006

AbstractThe gas‐phase ion pair SN2 reactions at saturated sulfur LiX + CH3SY → CH3SX + LiY (X, Y = F, Cl, Br, I) are investigated using the CCSD(T) calculations. The calculated results show that the reactions LiX + CH3SY are exothermic only when the nucleophile is a heavier lithium halide. Central barrier heights are found to depend primarily on the identity of nucleophile LiX, decreasing in the order LiF > LiCl > LiBr > LiI. Another interesting feature of the ion pair reactions at sulfur is the good correlation between the reaction barriers with geometrical looseness of LiX and SY bonds in the transition state structures. The data for the reaction barriers show good agreement with the prediction of the Marcus equation and its modification. © 2007 Wiley Periodicals, Inc. Int J Quantum Chem, 2007