bluuebunny/crossref_metadata_2025_split huggingface dataset:
HEPATIC SINUSOIDAL ENDOTHELIUM IN GOATS
Authors: Wright PL , Clemett JA , Smith KF , Day WA , Fraser R 
Published: August 2007

SummaryThe ultrastructure of the hepatic sinusoids of the goat was examined. Contrary to current belief concerning this and other ruminant species, we found the sinusoids to be lined by a smooth, continuous layer of endothelium, broken only by the presence of numerous, unoccluded fenestrae which provide a direct path of communication between the sinusoidal lumen and the space of Disse. Therefore, we suggest that a sieving function similar to that which has been shown to occur in rats may be attributed to the sinusoidal endothelium of the goat.
ISOANTIBODIES TO EPIDIDYMAL SPERM ANTIGENS IN BALB/c STRAIN OF MICE
Authors: Cukier D , Nayudu PRV 
Published: August 2007

SummaryFemale mice of the BALB/c isogenic strain were immunized with epididymal sperm of the male mice. Four antigenic perparations were used: whole sperm (WS), homogenized sperm (HS), protein fraction (PF) and lipid fraction (LF). Antisera were assayed for antibody activity by sperm immobilization and indirect immunofluorescence tests. The differential results between the WS and HS antisera obtained in the two tests suggest that sperm membrane surface antigen(s) may be distinct from intracellular antigen(s). PF‐antiserum showed lower but significant activity with the sperm immobilization test but weak activity with the indirect immunofluorescence test. The lipid fractions showed extremely weak activities with both tests. Indirect immunofluorescent staining was curried out on 10 μm sections of unfertilized and fertilized eggs and 2‐cell embryos. Unfertilized eggs showed no antibody binding and fertilized eggs showed patchy fluorescence of the plasma membrane and cytoplasm. In the 2‐cell embryo the fluorescence was diffuse, suggesting spreading of the sperm antigens. Comparative electrophoresis of aliquots of HS antiserum with and without incubation with washed sperm showed a clear reduction, due to adsorption by sperm, of IgG class of antibodies, on the basis of molecular weight. The differences in antigenicity between the four sperm preparations are discussed in relation to (i) possible localization of the antigens in the spermatozoa and (ii) the conformational differences of the antigens manifested by organic solvent denaturation.
DNALinux Virtual Desktop Edition
Authors: Bassi Sebastian , Gonzalez Virginia 
Published: August 2007

AbstractThe new version of DNALinux (VDE) is presented. DNALinux VDE is a departure from traditional distributions since it uses a virtual machine to bundle together the operating system and bioinformatics applications. The main advantage of this approach is that a virtualized environment doesn't affect a installed system. With a virtual machine a Linux system can be run under a Windows system, provided that the virtual machine player is installed. The included programs are listed and specifications to add more programs are explained. We believe that DNALinux could be used as a standardized virtual machine for learning, using, developing and testing bioinformatics applications.
An evaluation of counselling and rehabilitation courses for Chronic Fatigue Syndrome
Authors: Thomas Marie , Smith Andrew 
Published: August 2007

AbstractThe aim of the current study was to evaluate the services offered by Action for ME to sufferers of Chronic Fatigue Syndrome using measures developed and validated in previous research. Preliminary studies had suggested that clients attending rehabilitation residential courses were benefiting from the service. A further, more in‐depth evaluation process with a greater number of health‐related outcome measures was warranted. In addition, assessment was widened to include other services offered to sufferers of the illness. Data relating to the usefulness and success of the services (rated by the clients) were also collected. Data from client volunteers were collected at baseline (that is, before intervention) and approximately six months later. Quantitative comparative analyses were conducted using within‐group comparisons to assess any improvements in scores at six‐month follow‐up from baseline. Fifty‐six participants completed wide‐ranging questionnaires assessing illness history, psychopathology, psychosocial factors and health and well‐being. Data relating to improvements in illness status and acceptability of treatment were also collected by means of global outcome measures. Both the counselling and residential groups showed improvements in many areas assessed at follow‐up. Most importantly, improvements were indicated in areas such as fatigue and the levels of disability suffered by patients. In addition, there were significant improvements in ratings of mood, anxiety, depression and physical symptoms. Overall, clients reported satisfaction with the care received and most found the services useful. All of the participants who completed the evaluation stated that they would recommend Action for ME services to fellow sufferers. The outcome of the current study is encouraging. The data presented provides evidence of the high level of support and advice Action for ME offers to sufferers of this illness. Furthermore, measurable improvements in scores relating to illness status were accompanied by improvements in mental health and psychosocial variables in the patient group.
A pluralistic framework for counselling and psychotherapy: Implications for research
Authors: Cooper Mick , McLeod John 
Published: August 2007

AbstractHistorically, training, research and practice in counselling and psychotherapy have been dominated by unitary theoretical models. Although integrative and eclectic positions have been developed as alternatives, these have not been successful in generating research and have resulted in a further proliferation of competing models. In this paper we introduce a ‘pluralistic’ framework for counselling and psychotherapy and discuss the implications of this framework for research. The basic principle of this pluralistic framework is that psychological difficulties may have multiple causes and that there is unlikely to be one, ‘right’ therapeutic method that will be appropriate in all situations—different people are helped by different processes at different times. This pluralistic framework operates as a meta‐theory within which it is possible to utilise concepts, strategies and specific interventions from a range of therapeutic orientations. The framework is structured around three domains—goals, task and methods—by which therapeutic processes can be conceptualised, critically examined and empirically investigated. These domains, and the relationships between them, are outlined and the collaborative relationship at the heart of the pluralistic framework is discussed. The pluralistic framework provides a means for empirical research directly to inform practice and potential lines of empirical inquiry are outlined, along with findings from a recent study of counselling in schools.
Online self‐help: Developing a student‐focused website for depression
Authors: Meyer Denise 
Published: August 2007

AbstractMany depressed people, young men in particular, do not seek professional help. This social constructionist action research project aimed to construct a comprehensive self‐help website for students. Its goal was to give a multi‐layered (‘thick’) account of depression to website users offering empowering perspectives and strategies while challenging barriers to help‐seeking. Thirteen student interviewees, representing diverse demographics and depression experiences, attended semi‐structured interviews to elicit ‘coping’ narratives. Ten of them then formed an ongoing e‐mail focus group. An evaluative action research spiral was used to analyse and act upon public health guidelines, student interviews and student focus group commentary. Triangulation was provided via the specifications and ongoing feedback from the other project stakeholders. These were the funding charity steering group (including heads of university counselling services); the university providing programme/ethical approval; and the expert group offering clinical validation. The final site at www.studentdepression.org has nearly 100 pages of information and self‐help resources cross‐referenced with personal narratives. It provides a rich, complex account of how depression may be tackled and resisted. Both student and expert groups were impressed with final site quality and usefulness. Collaborative development with user‐group representatives is likely to have produced a far richer, more accessible and more comprehensive resource than counsellor authorship alone.1
‘Does class count?’ Social class and counselling
Authors: Ballinger Liz , Wright Jeannie 
Published: August 2007

AbstractThis article explores the importance attached to social class by experienced practitioners taking part in a co‐operative inquiry group. A review of the literature from the last thirty years indicates that there is very little research on class in relation to counselling and psychotherapy reported in the UK. Both authors position themselves as coming from working class origins. Nine co‐researchers from both middle and working class origins joined the group. Eight meetings took place over a period of nine months. Extracts from the group's discussions are represented and integrated with ‘presentational knowing’ drawn from contemporary culture, including poetry and popular music. This study suggests that social class is a neglected aspect of diversity in the counselling field. Implications of the study have relevance for the language of counselling and psychotherapy and class based values; social class and its impact on initial education; and ongoing counselling practice and access to therapy for working class people.
Generation of Single-Copy T-DNA Transformants in Arabidopsis by the CRE/loxP Recombination-Mediated Resolution System
Authors: De Buck Sylvie , Peck Ingrid , De Wilde Chris , Marjanac Gordana , Nolf Jonah , De Paepe Annelies , Depicker Ann 
Published: August 2007

Abstract
We investigated whether complex T-DNA loci, often resulting in low transgene expression, can be resolved efficiently into single copies by CRE/loxP-mediated recombination. An SB-loxP T-DNA, containing two invertedly oriented loxP sequences located inside and immediately adjacent to the T-DNA border ends, was constructed. Regardless of the orientation and number of SB-loxP-derived T-DNAs integrated at one locus, recombination between the outermost loxP sequences in direct orientation should resolve multiple copies into a single T-DNA copy. Seven transformants with a complex SB-loxP locus were crossed with a CRE-expressing plant. In three hybrids, the complex T-DNA locus was reduced efficiently to a single-copy locus. Upon segregation of the CRE recombinase gene, only the simplified T-DNA locus was found in the progeny, demonstrating DNA had been excised efficiently in the progenitor cells of the gametes. In the two transformants with an inverted T-DNA repeat, the T-DNA resolution was accompanied by at least a 10-fold enhanced transgene expression. Therefore, the resolution of complex loci to a single-copy T-DNA insert by the CRE/loxP recombination system can become a valuable method for the production of elite transgenic Arabidopsis thaliana plants that are less prone to gene silencing.
Genetic and Molecular Interactions between BELL1 and MADS Box Factors Support Ovule Development inArabidopsis
Authors: Brambilla Vittoria , Battaglia Raffaella , Colombo Monica , Masiero Simona , Bencivenga Stefano , Kater Martin M. , Colombo Lucia 
Published: August 2007

AbstractIn Arabidopsis thaliana and many other plant species, ovules arise from carpel tissue as new meristematic formations. Cell fate in proliferating ovule primordia is specified by particular ovule identity factors, such as the homeodomain factor BELL1 (BEL1) and MADS box family members SEEDSTICK (STK), SHATTERPROOF1 (SHP1), SHP2, and AGAMOUS. Both in the bel1 mutant and the stk shp1 shp2 triple mutant, integuments are transformed into carpelloid structures. Combining these mutants in a bel1 stk shp1 shp2 quadruple mutant, we showed that the bel1 phenotype is significantly enhanced. We also demonstrate that ovule differentiation requires the regulation of the stem cell maintenance gene WUSCHEL, repression of which is predominantly maintained by BEL1 during ovule development. Based on yeast three-hybrid assays and genetic data, we show that BEL1 interacts with the ovule identity MADS box factors when they dimerize with SEPALLATA proteins. We propose a model for ovule development that explains how the balance between carpel identity activity and ovule identity activity is established by a MADS box homeodomain protein complex.
MYB98 Positively Regulates a Battery of Synergid-Expressed Genes Encoding Filiform Apparatus–Localized Proteins
Authors: Punwani Jayson A. , Rabiger David S. , Drews Gary N. 
Published: August 2007

AbstractThe synergid cells within the female gametophyte are essential for reproduction in angiosperms. MYB98 encodes an R2R3-MYB protein required for pollen tube guidance and filiform apparatus formation by the synergid cells. To test the predicted function of MYB98 as a transcriptional regulator, we determined its subcellular localization and examined its DNA binding properties. We show that MYB98 binds to a specific DNA sequence (TAAC) and that a MYB98–green fluorescent protein fusion protein localizes to the nucleus, consistent with a role in transcriptional regulation. To identify genes regulated by MYB98, we tested previously identified synergid-expressed genes for reduced expression in myb98 female gametophytes and identified 16 such genes. We dissected the promoter of one of the downstream genes, DD11, and show that it contains a MYB98 binding site required for synergid expression, suggesting that DD11 is regulated directly by MYB98. To gain insight into the functions of the downstream genes, we chose five genes and determined the subcellular localization of the encoded proteins. We show that these five proteins are secreted into the filiform apparatus, suggesting that they play a role in either the formation or the function of this unique structure. Together, these data suggest that MYB98 functions as a transcriptional regulator in the synergid cells and activates the expression of genes required for pollen tube guidance and filiform apparatus formation.
The Organization of High-Affinity Ammonium Uptake in Arabidopsis Roots Depends on the Spatial Arrangement and Biochemical Properties of AMT1-Type Transporters
Authors: Yuan Lixing , Loqué Dominique , Kojima Soichi , Rauch Sabine , Ishiyama Keiki , Inoue Eri , Takahashi Hideki , von Wirén Nicolaus 
Published: August 2007

Abstract
The AMMONIUM TRANSPORTER (AMT) family comprises six isoforms in Arabidopsis thaliana. Here, we describe the complete functional organization of root-expressed AMTs for high-affinity ammonium uptake. High-affinity influx of 15N-labeled ammonium in two transposon-tagged amt1;2 lines was reduced by 18 to 26% compared with wild-type plants. Enrichment of the AMT1;2 protein in the plasma membrane and localization of AMT1;2 promoter activity in the endodermis and root cortex indicated that AMT1;2 mediates the uptake of ammonium entering the root via the apoplasmic transport route. An amt1;1 amt1;2 amt1;3 amt2;1 quadruple mutant (qko) showed severe growth depression under ammonium supply and maintained only 5 to 10% of wild-type high-affinity ammonium uptake capacity. Transcriptional upregulation of AMT1;5 in nitrogen-deficient rhizodermal and root hair cells and the ability of AMT1;5 to transport ammonium in yeast suggested that AMT1;5 accounts for the remaining uptake capacity in qko. Triple and quadruple amt insertion lines revealed in vivo ammonium substrate affinities of 50, 234, 61, and 4.5 μM for AMT1;1, AMT1;2, AMT1;3, and AMT1;5, respectively, but no ammonium influx activity for AMT2;1. These data suggest that two principle means of achieving effective ammonium uptake in Arabidopsis roots are the spatial arrangement of AMT1-type ammonium transporters and the distribution of their transport capacities at different substrate affinities.
A Ribonuclease III Domain Protein Functions in Group II Intron Splicing in Maize Chloroplasts
Authors: Watkins Kenneth P. , Kroeger Tiffany S. , Cooke Amy M. , Williams-Carrier Rosalind E. , Friso Giulia , Belcher Susan E. , van Wijk Klaas J. , Barkan Alice 
Published: August 2007

AbstractChloroplast genomes in land plants harbor ∼20 group II introns. Genetic approaches have identified proteins involved in the splicing of many of these introns, but the proteins identified to date cannot account for the large size of intron ribonucleoprotein complexes and are not sufficient to reconstitute splicing in vitro. Here, we describe an additional protein that promotes chloroplast group II intron splicing in vivo. This protein, RNC1, was identified by mass spectrometry analysis of maize (Zea mays) proteins that coimmunoprecipitate with two previously identified chloroplast splicing factors, CAF1 and CAF2. RNC1 is a plant-specific protein that contains two ribonuclease III (RNase III) domains, the domain that harbors the active site of RNase III and Dicer enzymes. However, several amino acids that are essential for catalysis by RNase III and Dicer are missing from the RNase III domains in RNC1. RNC1 is found in complexes with a subset of chloroplast group II introns that includes but is not limited to CAF1- and CAF2-dependent introns. The splicing of many of the introns with which it associates is disrupted in maize rnc1 insertion mutants, indicating that RNC1 facilitates splicing in vivo. Recombinant RNC1 binds both single-stranded and double-stranded RNA with no discernible sequence specificity and lacks endonuclease activity. These results suggest that RNC1 is recruited to specific introns via protein–protein interactions and that its role in splicing involves RNA binding but not RNA cleavage activity.
Applying general risk management principles to library administration
Authors: Joint Nicholas 
Published: August 2007

PurposeRecent areas of digital library innovation, such as digital rights management, have led librarians to apply risk management (RM) principles to certain circumscribed areas of library management. This paper will attempt to argue that RM principles can in fact be applied much more generally in modern library administration.Design/methodology/approachA conceptual paper based on abstract principles of RM.FindingsThat different approaches to RM apply in digital librarianship, as opposed to traditional, print‐based library work. Also, that different models of “RM” can be used at the level of the employee in contrast to the global perspective of the organisation as a whole.Research limitations/implicationsBecause this is a conceptual piece, there is ample room for further experimental testing of these hypotheses.Practical implicationsThis paper rejects some of the principles of RM that are less relevant to the “people management” problems of hybrid (print‐electronic) library administration. It offers an abstract statement of RM principles that should be of genuine practical usefulness to middle managers helping staff cope with the problems of “mixed media”, hybrid library environments.Originality/valueThis paper relates ideas from general business RM to practitioner librarianship in ways that have not been attempted previously.
Libraries and local history collections in Sierra Leone
Authors: Abdul Kargbo John 
Published: August 2007

PurposeDiscusses the development of local history collections within libraries in Sierra Leone.Design/methodology/approachThe article is a reflective viewpoint.FindingsThat there is a growing interest in local history collections in libraries in Sierra Leone, and that more formal structures should be put in place to safeguard these materials for the future.Practical implicationsProposes some ways forward for ensuring the long term viability of local collections in Sierra Leone.Originality/valueThe article is the first on the topic of local history collections in Sierra Leone's libraries, and should be of interest to librarians around the world who have an interest in local history librarianship.
Balancing individuals’ expectations and organisational requirements for continuing professional development
Authors: Cossham Amanda , Fields Alison 
Published: August 2007

PurposeThis paper aims to analyse the differing views on and needs of librarians and their managers for continuing professional development (CPD).Design/methodology/approachThis article draws on the findings of a survey conducted by the authors in 2005. The survey was an assessment of the CPD needs of New Zealand's librarians and library assistants.FindingsThere is a significant gap between the CPD that individuals want, and that which their managers think they should have. Organisations overall need a greater strategic focus on CPD to ensure that budgets are spent well, staff are appropriately skilled, and the impact of CPD on both individuals and the organisation is tangible. Individuals need to take more responsibility for ensuring their needs are met.Originality/valueHighlights a dual focus on CPD across the profession, in the light of the decision by the Library and Information Association of New Zealand Aotearoa to adopt professional registration.
Support for alternative publishing by public libraries in Scotland
Authors: Brookens Angela , Poulter Alan 
Published: August 2007

PurposeIt is proposed that public libraries have a duty to collect material from alternative publishers (in both fiction and non‐fiction and in all media) to better reflect the diversity of their communities. This paper aims to investigate the links between alternative publishing and public libraries in Scotland.Design/methodology/approachTwo surveys (based on the 1979 Alternative Acquisitions Project) were carried out of alternative publishers and public libraries in Scotland. Questions were based on those in the 1979 survey, except where updated to accommodate new technologies. A literature review was also carried out to contextualise survey findings.FindingsWhile alternative publishers and public libraries were aware of each other, alternative publishers faced many hurdles in getting their material in public libraries. For their part, public libraries were constrained by budgets but wanted to extend support for alternative publishing.Originality/valueThis paper re‐uses a previously tried and tested methodology to create a comparable and up to date study of an area of publishing often overlooked. Alternative publishing is revealed as a flourishing area, despite trends towards fewer and larger publishing outlets. Public libraries are seen as having a vital role to play in giving an outlet to alternative publishing.