bluuebunny/crossref_metadata_2025_split huggingface dataset:
Machine-learning-guided Directed Evolution for AAV Capsid Engineering
Authors: Fu Xianrong , Suo Hairui , Zhang Jiachen , Chen Dongmei 
Published: March 2024

Abstract:
Target gene delivery is crucial to gene therapy. Adeno-associated virus (AAV) has emerged as a primary
gene therapy vector due to its broad host range, long-term expression, and low pathogenicity. However,
AAV vectors have some limitations, such as immunogenicity and insufficient targeting. Designing or modifying
capsids is a potential method of improving the efficacy of gene delivery, but hindered by weak biological
basis of AAV, complexity of the capsids, and limitations of current screening methods. Artificial intelligence
(AI), especially machine learning (ML), has great potential to accelerate and improve the optimization of capsid
properties as well as decrease their development time and manufacturing costs. This review introduces the
traditional methods of designing AAV capsids and the general steps of building a sequence-function ML model,
highlights the applications of ML in the development workflow, and summarizes its advantages and challenges.
Enantioselective Zirconium-catalyzed Transformations
Authors: Pellissier Hélène 
Published: March 2024

Abstract:
The cheaper and less-toxic metals of group 4 compared with common metals
used in catalysis are increasingly applied in catalysis, resulting in the development of
many novel greener transformations. Zirconium is abundant, non-toxic, and exhibits a
remarkably diversified chemical reactivity among these metals. Since the first asymmetric
zirconium-catalyzed reaction disclosed by Nugent in 1992, a wide variety of chiral zirconium
catalysts have been proven to be capable of promoting many types of highly enantioselective
transformations, spanning from standard reactions, such as Friedel-Crafts reactions,
cycloadditions, aldol reactions, Mannich reactions, epoxidations, nucleophilic additions
to carbonyl compounds and derivatives, cyanations, ring-opening reactions, hydroxylations,
hydroformylations, carboaluminations among others, to more modern and complex
domino and tandem processes. This review aims to collect the major progress
achieved in the field of enantioselective transformations of all types promoted by chiral zirconium catalysts,
covering the literature since the beginning of 2003 and illustrating the power of these non-toxic catalysts to provide
high enantioselectivity in almost all kinds of asymmetric organic reactions. It is divided into ten parts, focussing
consecutively on enantioselective Friedel-Crafts reactions, cycloadditions, aldol reactions, Mannich
reactions, epoxidations, additions of alkylzinc reagents to imines, cyanations, ring-opening reactions, hydroxylations,
and domino/ tandem reactions. The diversity of these transformations well reflects that of the products
synthesized. For example, chiral indole and pyrrole derivatives were prepared from Friedel-Crafts reactions;
pyranones, pyridones and pyrazolidines from cycloadditions; β-hydroxy α-diazo carbonyl compounds, β-
hydroxy (thio)esters and β-hydroxy-α-amino acid derivatives from aldol reactions; β-amino (thio)esters from
Mannich reactions; functionalized epoxides from epoxidations; amines from additions of alkylzinc reagents to
imines; amino nitriles from cyanations; 1,2-diamines and β-vinyloxy alcohols from ring-opening processes; 2-
hydroxy 1-indanones from hydroxylations; various amines, 1,3-anti-diol monoesters, β-amino esters, α,β-
dihydroxy acid derivatives, α-amino ketones, indoles, cyclopentane and aryl α-aminophosphonates from domino/
tandem reactions. Furthermore, the utility of these novel methodologies was demonstrated in the total synthesis
of numerous essential bioactive products, such as (+)-prelactone C, (+)-9-deoxygoniopypyrone, (+)-
coniine, vancomycin, (+)-fusarisetin A, mycolipenic acid, onchidin, indoxacarb, tachykinin receptor antagonists,
cerebroprotecting agent MS-153, and L-erythro-sphingosine.

:
The advances achieved in the last three decades demonstrate that the non-toxicity, abundance, and efficiency of
zirconium make its application in catalysis suiting the growing demand for more environmentally benign processes,
offering the real opportunity to replace other toxic and expensive metals in the near future.
Recent Trends and Applications of Nanostructure-based Drug Delivery in Alleviating Chronic Obstructive Pulmonary Disease (COPD)
Authors: Lokesh  , Gulati Nisha , Saini Annu , Singh Sachin , Gupta Gaurav , MacLoughlin Ronan , Chellappan Dinesh Kumar , Dua Kamal , Dureja Harish 
Published: March 2024

Abstract:
Chronic Obstructive Pulmonary Disease (COPD), a chronic lung disease that causes
breathing difficulties and obstructs airflow from the lungs, has a significant global health burden
and affects millions of people worldwide. The use of pharmaceuticals in COPD treatment is aimed
to alleviate symptoms, improve lung function, prevent exacerbations, and enhance the overall quality
of life for patients. Nanotechnology holds great promise to alleviate the burden of COPD. The
main goal of this review is to present the full spectrum of therapeutics based on nanostructures for
the treatment and management of COPD, including nanoparticles, polymeric nanoparticles, polymeric
micelles, solid-lipid nanoparticles, liposomes, exosomes, nanoemulsions, nanosuspensions,
and niosomes. Nanotechnology is just one of the many areas of research that may contribute to the
development of more effective and personalized treatment modalities for COPD patients in the future.
Future studies may be focused on enhancing the therapeutic effectiveness of nanocarriers by
conducting extensive mechanistic investigations to translate current scientific knowledge for the
effective management of COPD with little or no adverse effects.
Research on Cascading Voltage Fault Ride-through Strategy for DFIG Based on Hybrid Energy Storage System
Authors: You Haichuan , Ding Can 
Published: March 2024

Background:
At present, clean energy power generation technology is vigorously developing,
and wind power generation technology is widely applied. Ensuring that the wind turbine
operation is not off-grid has become critical. Currently, most of the studies on fault ride-through
problems of doubly fed induction generators (DFIG) are single faults, i.e., low voltage ride
through (LVRT) or high voltage ride through (HVRT), mostly ignoring the low and high voltage
cascading fault.

background:
With the gradual increase of wind power penetration rate, how to ensure that the wind turbine operation is not off-grid has become critical. Currently, most of the studies on fault ride-through problems of doubly fed induction generator (DFIG) are single faults, i.e., low voltage ride through (LVRT) or high voltage ride through (HVRT), mostly ignoring the low and high voltage cascading fault

Methods:
This paper proposes a joint control strategy based on hybrid energy storage (HESS) to
cope with the cascading fault ride-through requirement. During the fault period, the DFIG rotor
side converter (RSC) works in the reactive power priority mode to provide reactive power support
for the power grid. In view of the hysteresis of the traditional PI control of HESS, Model predictive
control is used to improve.

Results:
During the fault traversal process, the DC voltage of the fan is stable, the output waveform
is smooth, and the support capacity is significantly improved.

method:
During the fault period, the DFIG rotor side converter (RSC) works in the reactive power priority mode to provide reactive power support for the power grid. In view of the hysteresis of the traditional PI control of HESS, Model predictive control is used to improve.

Conclusion:
Simulation results show that the proposed joint control strategy can effectively
support grid voltage recovery and maintain stable DC bus voltage, effectively achieving DFIG
low and high voltage continuous fault crossing.

conclusion:
Simulation results show that the proposed joint control strategy can effectively support grid voltage recovery and maintain stable DC bus voltage, effectively
Artificial intelligence in molecular and genomic prostate cancer diagnostics
Authors: Morozov A. O. , Bazarkin A. K. , Vovdenko S. V. , Taratkin M. S. , Balashova M. S. , Enikeev D. V. 
Published: March 2024

Introduction. Many molecular genetic analyses have been proposed to predict the course of prostate cancer (PCa). They have the potential to develop artificial intelligence (AI) algorithms by processing large amounts of data and define connections between them.Objective. To evaluate the possibilities of using artificial intelligence in early diagnosis and prognosis of prostate cancer.Materials & methods. We conducted a systematic review of the literature on the Medline citation database. We have selected papers that provide data on the use of AI in vitro, in vivo and in silico systems to determine biological and genetic markers and/or their relationship to clinical data of PCa-patients from 2020 to 2023. The quantitative synthesis includes 16 articles.Results. AI can identify metabolic and genetic «signature» of PCa, the key elements of signal pathways, thus fulfilling complex tasks in the field of bioinformatics. AI analyses various biomaterials: prostate tissue, blood, and urine. When evaluating prostate tissue for aberrations, AI can help a pathologist. For example, AI can predict the histological status of genes, eliminating the need for IHC or tissue sequencing, significantly reducing the economic cost of predicting the severity of the disease. In most cases, prostate tissue sequencing provides information to the attending physician, allowing the start of optimal treatment, considering the molecular or genetic «signature» of PCa. AI can be used as an alternative to existing population screening tools and a predictive castration-resistant PCa. The use of AI capabilities is more appropriate for blood and urine analysis, procedures that do not require additional economic costs for biomaterial sampling. In theory, this may be more affordable for the patient and the medical institution. It is worth noting that a few studies were conducted in silico (based on the analysis of molecular genetic databases without validation on cell lines or on real patients) and are useful as background information. However, the results can serve as a robust basis for further research in molecular diagnostics and genomics.Conclusion. It is possible to use AI in the search for key metabolites and genes of the elements of signalling pathways, as well as the determination of metastasis potential, because molecular or genetic «signature» of PCa allows the physician to start optimal treatment.
Лікарські засоби в стоматології: аналіз застосування у 2023 році
Authors: Mazur I.P. , Stadnyk M.B. , Ventsuryk Y.O. , Mazur P.V. , Buria D.A. 
Published: March 2024

Актуальність. Щодня лікарі-стоматологи стикаються з питанням призначення пацієнтам різних лікарських засобів, а оптимальне рішення ґрунтується на глибокому розумінні фармакотерапії. На сьогодні фармацевтичний ринок пропонує широкий спектр препаратів, регулярно доповнюючи його новими, проте питання раціональності та оптимізації їх використання залишається актуальним для всіх. Мета дослідження: провести аналіз статистичних даних стосовно частоти і призначення лікарями-стоматологами антибактеріальних, нестероїдних протизапальних, антисептичних препаратів та засобів гігієни ротової порожнини у різних клінічних випадках. Матеріали та методи. Дослідження містить результати опитування 1423 респондентів — практикуючих лікарів-стоматологів з усіх регіонів України стосовно частоти та призначення лікарських засобів. Опитування проводились під час науково-практичних конференцій в онлайн-режимі у Ι півріччі 2023 року, що були організовані ГО «Асоціація стоматологів України». Результати. Результати опитування продемонстрували, що у своїй практиці лікарі-стоматологи щоденно використовують різні фармакотерапевтичні препарати, із них 43,71 % лікарів-стоматологів призначають пацієнтам комбіновані антибактеріальні препарати (ципрофлоксацин і тинідазол), 39,56 % призначають пеніцилін, 29,23 % — метронідазол. Для лікування больового синдрому найчастіше лікарі-стоматологи використовують німесулід (90,16 %). У разі необхідності місцевого лікування інфекційно-запальних процесів у порожнині рота використовують: хлоргексидин (69,01 %), Метрогіл Дента (66,27 %), Холісал (43,57 %). Проведено аналіз статистичних даних щодо призначень засобів індивідуальної гігієни порожнини рота — зубних щіток, зубних паст, ополіскувачів для порожнини рота. Висновки. Результати аналізу проведеного опитування свідчать про широке використання фармакотерапевтичних препаратів лікарями-стоматологами у щоденній практиці. Обізнаність стосовно застосування нових лікарських засобів лікарів-стоматологів буде сприяти досягненню клінічно передбачуваних та високоефективних результатів лікування.
Los cabildos de las catedrales indianas, siglos XVI y XVII
Authors: Pérez Puente Leticia 
Published: March 2024

Los cabildos de las catedrales eran corporaciones de clérigos dotadas de personalidad jurídica, cuyo principal objetivo era la celebración del culto divino en el coro de la catedral, administrar las rentas eclesiásticas, y asesorar a los obispos en la carga pastoral y la dirección de los tribunales eclesiásticos. Ya que al inicio estuvieron sujetos al arzobispado de Sevilla, los cabildos indianos siguieron sus ceremonias y rituales con las costumbres hispalenses. El trabajo expone el problema de las catedrales americanas, que no podían cumplir con la liturgia sevillana por sus condiciones precarias y la inestabilidad que les generaban las constantes batallas entre indios y conquistadores.
Acceso a la información como primer escalón en las etapas de inversión para la reducción efectiva de consumo de energía
Authors: Ducón-Sosa Vladimir Camilo , Amézquita-Tovar Juan Carlos 
Published: March 2024

Hoy en día, el mundo y Colombia se enfrentan a una de las crisis más visibles e impactantes debido al aumento de la temperatura. Esta crisis nos hace cuestionarnos sobre cómo vamos a enfrentar los próximos 50 años. Bueno, algunos síntomas son realmente notorios desde la perspectiva ambiental. El cambio climático es un hecho con el que vamos a vivir, y no va a desaparecer en el corto plazo. El aumento de la temperatura de la tierra traerá consigo numerosos inconvenientes. Algunas de ellas se han manifestado desde hace algunos años. El rápido cambio de la temperatura está provocando sequías severas, inundaciones, tormentas eléctricas, tornados, erosión del suelo, huracanes, calor extremo, incendios forestales y otro tipo de desastres naturales. Estas situaciones son producidas por un desequilibrio en el equilibrio energético de la tierra. Este desequilibrio es, sin duda, causado por la acción humana como resultado de las actividades económicas aceleradas.
Por lo tanto, estamos tratando de entender el marco general de las causas y posibles soluciones para la vida humana en la tierra durante los próximos 100, 50 o 20 años. ¿Cómo podemos, como principal causa del daño al hábitat, mitigar algunos de estos problemas importantes y relevantes que creamos y que ponen en peligro nuestra supervivencia en el planeta tierra?
Este documento proporciona información específicamente sobre la cadena de suministro de energía eléctrica y agua. Trata de describirlo desde una perspectiva general o global hasta una perspectiva local o regional. Vamos a fomentar el reconocimiento de cómo se proporcionan estos recursos relevantes y cómo todo el proceso puede contribuir a la emisión de gases de efecto invernadero o afectar la sostenibilidad de las comunidades.
Este documento también enfatizará lo importante que es reconocer y entender el problema desde diferentes perspectivas para comenzar a sugerir soluciones utilizando tecnologías 4.0 como herramientas eficientes para comenzar a tomar medidas para mitigar algunas de las causas a nivel local. El potencial de optimizar el uso de recursos y reducir.
SISTIM PAKAR DIAGNOSIS GANGGUAN PADA KEHAMILAN TRIMESTER PERTAMA MENGGUNAKAN METODE CERTAINTY FACTOR BERBASIS WEB
Authors: Tokan Diana Inda Carmilla , Mola Sebastianus Adi Santoso , Widiastuti Tiwuk 
Published: March 2024

Gangguan kehamilan adalah masalah yang terjadi selama masa kehamilan, biasanya disebabkan oleh perubahan hormon dalam tubuh ibu dan perkembangan bayi di dalam rahim. Menurut World Health Organization (WHO), sekitar 15% dari seluruh wanita yang hamil mengalami komplikasi yang dapat mengancam nyawa mereka. Berdasarkan data Puskesmas Oepoi tercatat sekitar 17,19% ibu hamil mengalami gangguan pada kehamilan trimester pertama atau sekitar 1 hingga 12 minggu kehamilan saat diperiksa. Untuk membantu dan memudahkan ibu hamil mendapatkan informasi mengenai gangguan pada kehamilan, penelitian ini menggunakan sistem pakar. Sistem ini membantu tenaga medis dan masyarakat dalam mendiagnosis gangguan pada kehamilan trimester pertama berdasarkan gejala yang dirasakan. Sistem ini menggunakan metode certainty factor sebagai mesin inferensi untuk menghitung dan mengetahui derajat kepercayaan terhadap gangguan yang dialami. Terdapat 5 jenis gangguan dan 39 gejala yang dapat didiagnosis oleh sistem berdasarkan keluhan pasien. Berdasarkan pengujian dengan 100 sampel data rekam medis pasien gangguan pada kehamilan trimester pertama, sistem ini terbukti memiliki tingkat akurasi sebesar 94%.
Pengaruh Bimbingan Orang Tua Meningkatkan Konsep Diri Remaja di Bumiharjo Kecamatan Keling Kabupaten Jepara
Authors: Saliyo Saliyo Saliyo 
Published: March 2024

Penelelitian ini bertujuan untuk menguji pengaruh bimbingan orang tua terhadap konsep diri remaja. Dalam penelitian ini terdapat satu variabel bebas (independen) yaitu bimbingan orangtua (X) dan satu variabel terikat (dependen) yaitu, konsep diri (Y). Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kuantitatif dengan teknik pengambilan data menggunakan skala, wawancara dan dokumentasi. Pengambilan sampel penelitian ini menggunakan teknik random sampling, dengan jumlah sampel yang digunakan sebanyak 150 remaja.Hasil penelitian menunujukkan: Pertama, terdapat pengaruh bimbingan orang tua terhadap konsep diri remaja di Desa Bumiharjo, Kecamatan Keling Kabupaten Jepara. Hasil uji regresi linear sederhana menunjukan bahwa (sig. 0,000 0,05). Hal tersebut menunjukkan bahwa variabel independen (bimbingan orang tua) memiliki pengaruh yang signifikan terhadap variabel dependen (konsep diri remaja). Kedua, berdasarkan hasil uji koefisien determinasi (R2) ditentukan dengan nilai R square sebesar 0,248 atau 24,8%. Nilai tersebut menunjukkan pengaruh bimbingan orang tua terhadap konsep diri remaja sebanyak 24,8% dan 75,2% selebihnya diperngaruhi oleh variabel lain. Adapun nilai R sebesar 0,498 dan berarti bahwa bimbingan orang tua memiliki tingkat korelasi terhadap konsep diri sedang (interval 0,40–0,599).
Patron Client in Political Corruption in Circles South Sulawesi BUMD ((Regional Owned Enterprises))
Authors: Amin Rahma , Sulaiman Herlina , Muin Indriani 
Published: March 2024

Purpose – The aim of this research is to understand the forms and processes of political corruption within BUMDs based on patron-client relationships in placing people in strategic positions in the company.
Method - This research uses secondary data, namely based on the results of the anti-corruption assessment carried out by TI Indonesia. Then carry out a literacy study to draw conclusions and verify.
Result - The political corruption nested within BUMDs in South Sulawesi has had an impact on unsatisfactory performance and achievements, where continuous losses have been found, which is the impact of the recruitment system for BUMD directors which is only based on the politics of retaliation in the regional elections.
Implication – Transparency International (TI) Indonesia in the period December 2022-January 2023 has carried out a Transparency in Corporate Reporting assessment of 47 companies or Regional Owned Enterprises (BUMD) in 5 Provinces, South Sulawesi (Sulsel) is one of the provinces assessed. The result was that five South Sulawesi BUMDs were categorized as sick and not transparent in terms of management, making them prone to corruption. Through this research, it is hoped that it can be an evaluation of the performance of BUMDs in South Sulawesi related to company management.
Pengaruh Digital Marketing terhadap Minat Beli Produk Skincare Lokal di Kecamatan Belopa
Authors: Mursyid Mursyid , Julita Bunga 
Published: March 2024

This study aims to examine how customers' inclination to purchase local skincare products is affected by social media marketing and electronic word-of-mouth. The major objective of this study is to ascertain whether electronic word-of-mouth and social media marketing significantly affect local skincare consumers' propensity to buy items, either individually or collectively.
Quantitative research is used, and measures are made using a Liker scale. The population in this study are users of local skincare products in Belopa District with an undetermined population size. 100 respondents made up the sample, which was selected using a straightforward random sampling method and the Lemeshow algorithm. By handing out questionnaires and disseminating them online and in person, survey data was collected. SPSS is used for data processing and analysis methods 24 program tools.
The findings of this study demonstrate that there is a significant simultaneous influence between electronic word of mouth and social media marketing on buying interest in local skincare, with a T_count value of 5.331 > T_table 1.985, a partially significant influence of social media marketing on buying interest in local skincare, and a T_count sig value of 5.183 > T_table 1.985.
The Effect of Digital Marketing on Purchase Loyalty with Customer Satisfaction as an Intervening Variable on Products Starbucks in Sukoharjo
Authors: Santoso Bangkit Adjie , Lestari Wuryaningsih Dwi 
Published: March 2024

The purpose of this study was to determine the effect of digital marketing on purchase loyalty with customer satisfaction as an intervening variable on Starbucks products in Sukoharjo. This research method is quantitative. The population in this study was Starbuck Coffee consumers in Sukoharjo. The sampling technique for this research is nonprobability sampling. The sample in this study amounted to 100 respondents. The data analysis technique in this study uses Partial Least Square (PLS) using the help of SMARTPLS Software. The results of this study are digital marketing has a significant positive effect on purchase loyalty. Digital marketing has a significant positive effect on customer satisfaction. Customer satisfaction has a significant positive effect on digital marketing. The conclusion of this study is that digital marketing affects purchase loyalty with customer satisfaction as an intervening variable on Starbucks products in Sukoharjo
TELAAH KRITIS PENGATURAN TENTANG YAYASAN DI INDONESIA
Authors: Jamaluddin Fitriani 
Published: March 2024

The purpose of this study is to examine Law No. 16 of 2001 concerning Foundations in conjunction with Law No. 28 of 2004 concerning Foundations. The results showed that the basic purpose of establishing the foundation for social, humanitarian, and religious interests in implementation was not in accordance with the provisions of the Foundation Law, besides that there was no sanction for Foundation organs that took advantage of personal interests in the management of the Foundation.