bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcyclin-Binding Protein Inhibits Proliferation, Tumorigenicity, and Invasion of Gastric Cancer
Authors: Ning Xiaoxuan , Sun Shiren , Hong Liu , Liang Jie , Liu Lili , Han Shuang , Liu Zhiguo , Shi Yongquan , Li Yuan , Gong Weiqin , Zhang Shanhong , Chen Yu , Guo Xueyan , Cheng Yi , Wu Kaichun , Fan Daiming 
Published: January 2008

Abstract
Calcyclin-binding protein/Siah-1–interacting protein (CacyBP/SIP), a target protein of the S100 family, which includes S100A6, S100A1, S100A12, S100B, and S100P, has been identified as a component of a novel ubiquitinylation complex leading to β-catenin degradation. However, the function of CacyBP/SIP in gastric cancer has not been elucidated. In the present study, we prepared CacyBP/SIP overexpressing and knockdown cell lines of gastric cancer. Forced CacyBP/SIP expression inhibited the proliferation of gastric cancer cells, suppressed tumorigenicity in vitro, and prolonged the survival time of tumor-bearing nude mice. In addition, increased CacyBP/SIP repressed the invasive potential of gastric cancer cells. Conversely, the down-regulation of CacyBP/SIP by RNA interference showed the opposite effects. Further studies showed that depressed CacyBP/SIP increased the expression of total and nuclear β-catenin at the protein level and elevated the transcriptional activity of Tcf/LEF. Taken together, our results suggest that CacyBP/SIP may be a potential inhibitor of cell growth and invasion in the gastric cancer cell, at least in part through the effect on β-catenin protein expression and transcriptional activation of Tcf/LEF. (Mol Cancer Res 2007;5(12):1254–62)
Muscle formation during embryogenesis of the polychaete Ophryotrocha diadema (Dorvilleidae) – new insights into annelid muscle patterns
Authors: Bergter Annette , Brubacher John L , Paululat Achim 
Published: January 2008

Abstract

Background
The standard textbook information that annelid musculature consists of oligochaete-like outer circular and inner longitudinal muscle-layers has recently been called into question by observations of a variety of complex muscle systems in numerous polychaete taxa. To clarify the ancestral muscle arrangement in this taxon, we compared myogenetic patterns during embryogenesis of Ophryotrocha diadema with available data on oligochaete and polychaete myogenesis. This work addresses the conflicting views on the ground pattern of annelids, and adds to our knowledge of the evolution of lophotrochozoan taxa.

Results
Somatic musculature in Ophryotrocha diadema can be classified into the trunk, prostomial/peristomial, and parapodial muscle complexes. The trunk muscles comprise strong bilateral pairs of distinct dorsal and ventral longitudinal strands. The latter are the first to differentiate during myogenesis. They originate within the peristomium and grow posteriorly through the continuous addition of myocytes. Later, the longitudinal muscles also expand anteriorly and form a complex arrangement of prostomial muscles. Four embryonic parapodia differentiate in an anterior-to-posterior progression, significantly contributing to the somatic musculature. Several diagonal and transverse muscles are present dorsally. Some of the latter are situated external to the longitudinal muscles, which implies they are homologous to the circular muscles of oligochaetes. These circular fibers are only weakly developed, and do not appear to form complete muscle circles.

Conclusion
Comparison of embryonic muscle patterns showed distinct similarities between myogenetic processes in Ophryotrocha diadema and those of oligochaete species, which allows us to relate the diverse adult muscle arrangements of these annelid taxa to each other. These findings provide significant clues for the interpretation of evolutionary changes in annelid musculature.
TEL/AML1+ ACUTE LYMPHOBLASTIC LEUKEMIA IN THE GREEK PEDIATRIC POPULATION
Authors: Papadhimitriou Stefanos , Paterakis Georgios S. , Parcharidou Agapi , Tsakiridou Anastasia , Papadakis Vassilios , Papargyri Sofia , Paissiou Anna , Tourkantoni Natalia , Tsitsikas Konstantinos , Pavlidis Dimitris , Georgiakaki Maria , Polychronopoulou Sophia 
Published: January 2008

INTRODUCTION: TEL/AML1+ acute lymphoblastic leukemia (ALL) is considered to be a distinct nosological entity with excellent prognosis, but recent studies have indicated significant clinical heterogeneity.
OBJECTIVE: In this study, we attempted to estimate the incidence and clinical features of TEL/AML1+ ALL for the first time in a representative cohort of Greek pediatric patients.
METHODS: One hundred twenty children (&lt;16 years old) diagnosed with ALL (107 of B-cell origin, 13 of T-cell origin) were screened for TEL/AML1 with interphase fluorescence in situ hybridization by using a commercial probe set. All patients were treated as either standard risk (SR) or high-risk (HR) cases according to a modified BFM-95 (Berlin-Frenkfurt-Munster) protocol. Follow-up ranged between 5 and 87 months (median: 45 months).
RESULTS: Twenty-six patient (all of them will ALL of B-cell origin [24.3%]) were found to be positive for TEL/AML1. The presence of TEL/AML1 was significantly associated with younger age and lower white blood cell count at diagnosis but not with remission duration or overall survival rate. The number of children who relapsed (1 vs 7) or succumbed (1 vs 5) was comparable between the TEL/AML1+ and TEL/AML1− groups of children with ALL of B-cell origin.
CONCLUSIONS: The incidence of TEL/AML1 in Greece seems comparable to that in other European and Mediterranean countries. As in most European studies, the independent prognostic value of TEL/AML1 is in doubt, because it is closely associated with other favorable factors. In this series, the modification of the therapeutic regimen (ie, omission of the SR arm) may be responsible for the similar outcome in TEL/AML1+ and TEL/AML1− cases, because it seems to lower the relapse risk for all children with ALL.
PLACENTAL ADENOVIRAL GENOME: POLYMERASE CHAIN REACTION DETECTION AND PLACENTAL HISTOLOGY IN PRETERM AND TERM INFANTS
Authors: Tsekoura Eufrosini , Konstantinidou A. , Tsakris A. , Antsaklis A. , Kafetzis D. 
Published: January 2008

INTRODUCTION: Intrauterine infection is an important cause of spontaneous preterm birth. However, evidence-based etiology for the causative role of viral infection is still lacking. Intervillous trophoblasts express adenovirus receptor. Infection of trophoblast cells in vitro by adenovirus early in pregnancy has shown increased apoptosis. Adenovirus early in pregnancy may cause placental dysfunction. Mature syncytiotrophoblasts do not express adenovirus receptor.
OBJECTIVE: The aim of this study was to test the hypothesis that detection of adenovirus in placental tissue is associated with preterm birth and correlates with placental histopathological findings that are suggestive of infection.
METHODS: Placentas were prospectively collected from consecutive deliveries. Detection of the adenovirus genome was tested by polymerase chain reaction assay. Placental histology and immunohistochemistry studies with monoclonal antibody CD45 were evaluated for signs of placental inflammation in the samples that tested positive for adenovirus.
RESULTS: Between January 2005 and December 2006, 193 placenta samples (71 from preterm deliveries and 122 from term deliveries) were collected in Alexandra's Maternity Hospital in Athens, Greece. The adenoviral genome was isolated in 54 (28%) of 193 placentas. The frequency of adenovirus detection in preterm placentas compared with those from term placentas was significantly increased (29 of 71[41%] vs 25 of 122 [20%]; P = .002; odds ratio [OR]: 2.6 [95% confidence interval (CI): 1.4–5.1]). Stratification by gestational age (GA) revealed a stronger association between preterm delivery and adenovirus detection as GA decreased below 33 weeks (GA ≤ 29 weeks, OR: 2.8 [95% CI: 1.1–7.0]; and GA 30–33 weeks, OR: 2.7 [95% CI: 1.1–6.5]). In the subgroup of deliveries at 34 to 36 weeks' GA, the association was no longer significant (OR: 2.6 [95% CI: 0.9–7.0]). Adenoviral genome detection followed the seasonal variation of adenovirus respiratory infections (beginning of March to end of June). Chorioamnionitis was present more frequently in the adenovirus-positive preterm placentas compared with term placentas (P = .006). The presence of villitis (P = .03) and chorioamnionitis (P = .02) was significantly increased in the adenovirus-positive preterm placentas compared with preterm adenovirus-negative placentas.
CONCLUSIONS: Our results indicate that there is an association between placental adenoviral genome detection and spontaneous early premature birth. Adenovirus may cause preterm birth through placental inflammation (chorioamnionitis and villitis).
DOES INTRAFAMILIAL SPREAD PLAY A ROLE FOR HELICOBACTER PYLORI INFECTION IN CHILDREN?
Authors: Roma Eleftheria , Panayiotou Joanna , Pachoula Joanna , Kafritsa Yota , Constantinidou Cathrin , Mentis Andreas , Syriopoulou Vasiliki 
Published: January 2008

INTRODUCTION: Acquisition of Helicobacter pylori (Hp) infection in children occurs mainly in those under 5 years of age.
OBJECTIVE: Our aim was to investigate intrafamilial spread of Hp infection.
METHODS: One hundred symptomatic children without previous eradication treatment were investigated by gastroscopy and the 13C-urea breath test (UBT). All family members of each index patient were investigated by using the UBT. Infected members were estimated according to UBT results, and for those members who were UBT-negative and had recently received eradication therapy after confirmation of infection by endoscopy, the previous positivity was taken into account.
RESULTS: Ηp infection was identified in 44 (44%) of 100 symptomatic index children. There was no statistical difference between Ηp+ and Ηp− index children concerning demographic factors except age, which was higher in Ηp+ index children (P = .009). In all Ηp+ index children (100%) and in 71.4% of Ηp− children, at least 1 more family member was infected (P &lt; .001); in all cases (100%) at least 1 parent in the group of Ηp+ index children, compared with 69.6% in Ηp− index children (P &lt; .001), was infected. The rate of infected siblings of the Ηp+ index children was 43.2%, and that in the Ηp− group was 3.6% (P &lt; .001). There were more infected mothers in the Ηp+ index children group (83.7% vs 50% in the Ηp− group; P = .001) and more infected fathers (76.7% vs 56.4%, respectively; P = .035).
CONCLUSIONS: The identification of at least 1 more infected member in each family of Hp+ index patients, including a parent in all cases, strongly indicates family as the main source of infection for children and confirms the hypothesis of intrafamilial spread of Hp.
MONOCYTES EXPRESSING TISSUE FACTOR AS A DIAGNOSTIC MARKER FOR NEONATAL SEPSIS
Authors: Youssry Ilham , Edris Amira , Tawfik Naglaa , Rizk Samia , Abou-Elew Heba , Mokhtar Doha , El Messery Lobna 
Published: January 2008

INTRODUCTION: For neonatal sepsis, several clinical and laboratory parameters have been proposed for its diagnosis but with variable sensitivity and specificity. The bacterial products in sepsis, including endotoxin, induce the production of proinflammatory cytokines that evoke the expression of tissue factor (TF) on monocytes and endothelial cells.
OBJECTIVE: Our goal was to estimate the percentage of monocytes that express TF (TF%) by flow cytometry in patients with neonatal sepsis and to delineate its significance in diagnosing neonatal sepsis.
METHODS: Twenty-seven neonates with neonatal sepsis and positive blood-culture results were recruited and evaluated clinically for their risk factors. Laboratory investigations including obtaining complete blood count and C-reactive protein level and estimation of the monocytes' TF expression by flow cytometry were performed. Twenty-four normal newborns were included as controls for the laboratory data.
RESULTS: The monocytes that expressed TF% of the studied patients was significantly higher than that of the controls (P = .0001). The level of TF% was significantly influenced positively by premature rupture of membranes, multiplicity, white blood cell (WBC) count, staff/segment ratio, and C-reactive protein level and negatively by gestational age, body weight, and platelet count. The sensitivity and overall accuracy of the TF% were higher than those of the staff/segment ratio and the WBC count for diagnosing neonatal sepsis. The areas under the receiver operating characteristic curve of TF%, staff/segment ratio, and WBC count were 0.84, 0.79, and 0.60, respectively.
CONCLUSIONS: The monocytes expressing TF% is a promising diagnostic and prognostic marker of infection in neonatal sepsis with high sensitivity and overall accuracy. Adding the estimation of monocytes expressing TF% to the sepsis screen may improve the diagnosis of neonatal sepsis.
DOUBLE-BLIND TRIAL OF FORMULA IN DISTRESSED AND REGURGITATING INFANTS
Authors: Vandenplas Yvan , Devreker T. , Hauser B. 
Published: January 2008

INTRODUCTION: Many parents seek medical help because of frequent regurgitation and inconsolable crying of their formula-fed infant.
OBJECTIVE: We aimed to assess the effectiveness of special formulas in distressed and regurgitating infants.
METHODS: We included 12 consecutive infants in a prospective, randomized, single-blinded (parents), cross-over trial (6 infants were started on G1 [80/20 casein/whey, tapioca starch, and locust bean] for 2 weeks and then switched for 2 weeks to G3 [partial whey hydrolysate, tapioca starch, and locust bean]; for the other 6 infants, the order of the formulas was opposite). Infants were exclusively formula fed, were crying for &gt;3 hours/day for at least 3 weeks, and regurgitated several times after each feeding. Before inclusion, all of them had been given ≥3 different commercialized AR formulae, formulae for digestive comfort, and at least 1 extensive hydrolysate without success. All infants had been treated without success with a prokinetic agent (domperidone/cisapride) and an acid-blocking drug (H2-receptor agonist/proton-pump inhibitor).
RESULTS: Gastric emptying time, as evaluated with a 13C acetate breath test, was 117.1 ± 18.3 minutes with Novalac-AR (80/20 casein/whey and corn starch), 104.5 ± 15.5 minutes with G1, and 79.2 ± 14.0 minutes with G3 (P &lt; .001 [Friedman test]). The mean number of regurgitations per day was 5.1 ± 1.2 with G1 and 1.8 ± 1.2 with G3 (P = .002). Quality of life, as assessed by the parents in a diary, was 4.20 ± 1.79 with G1 and 2.10 + 0.74 with G3 (P = .005). The mean duration of crying per day was 84.5 ± 50.1 minutes with G1 and 26.7 ± 18.1 minutes with G3 (P = .003).
CONCLUSIONS: The cross-over design protected for bias. G3 scored better than G1 for all parameters evaluated and decreased regurgitation and infant distress significantly.
PREVALENCE OF SEXUAL ABUSE AMONG ADOLESCENT MEDICAL AND NURSING STUDENTS IN A COLLEGE IN PUNJAB, INDIA
Authors: Sobti Praveen , Biswas Gautam 
Published: January 2008

OBJECTIVE: We sought to determine the prevalence of sexual abuse among adolescent medical and nursing students who were attending a college in Punjab, India.
METHODS: Five hundred thirty-two subjects (150 male medical students, 220 female medical students, and 162 female nursing students) between 17 and 26 years old filled out a detailed questionnaire, in absolute privacy, to gather demographic and sexual abuse information.
RESULTS: of the participating students, 28.2% were male and 71.8% were female. The overall prevalence rate of any form of sexual abuse was 32.1% (171 subjects). A severe form of sexual abuse (intercourse) was reported by 30 subjects (10 among males and 20 among females). The most common type of abuse reported was in the form of showing pornographic material among male students (41 subjects [23%]) and touching of breasts (58 subjects [33.9%]) among females. The minimum age of victims at the time of the first incident was &gt;16 years in 83 subjects (48.5%). The abuser was a friend in 32.1% (29 in males and 27 in females) of the cases. The perpetrators of sexual abuse were males for 132 subjects of both genders. In 56% (96 subjects), the time of abuse was between 2 and 10 pm. Forty-one subjects (23%) currently experience abuse memories in the form of unwanted scenes flashing in dreams, difficulty in maintaining relationships, feeling of guilt, fear, or nausea when touched, and disturbing sexual thoughts, alone or in combination.
CONCLUSIONS: These results provide baseline information on the prevalence and type of sexual abuse among adolescents. Abuse involved both genders, but the perpetrators were male. For nearly one fourth of the students the incident seems to have left a long-term psychological impact on the victim.
PEDIATRICIANS' AWARENESS OF AND ATTITUDES ABOUT OTITIS MEDIA: RESULTS OF A MULTINATIONAL SURVEY
Authors: Arguedas Adriano , Lefebvre Cedric , Vercruysse Severine , Dean Carol , Maudrich Julie 
Published: January 2008

INTRODUCTION: Otitis media (OM) is a common problem in primary care and constitutes a significant health burden in &lt;5-year-olds. Middle-ear effusion after acute OM is also a common problem that causes hearing loss in a substantial proportion of children and is a frequent reason why primary care providers refer children to specialists. However, there are limited non-US data on pediatricians' awareness and attitudes toward OM disease burden, complications, and causative pathogens.
OBJECTIVE: A multinational survey was undertaken to validate and measure primary care physicians' attitudes and behaviors toward OM.
METHODS: Two thousand pediatricians from 10 countries (4 European, 3 Asian, 2 Latin American, and 1 Middle Eastern) were interviewed. Questions focused on the number of children younger than 5 who were treated for OM in the previous year, perceptions about complications and sequelae, awareness of OM pathogens, and concerns about current disease-management practice.
RESULTS: Reported estimates of OM in &lt;5-year-olds was 349 (range: 125–1000) cases per year per practice (ie, pediatricians treated at least 1 patient with OM per day). Eighty-two percent of the pediatricians reported that they treat OM with first-line antibiotics; they were generally satisfied but viewed antibiotic resistance as a serious issue. Nineteen percent of children were referred to an ear, nose, and throat specialist because of treatment failure, recurrent/chronic OM, or hearing problems or for surgery. Pediatricians associated OM with 2 main pathogens: Streptococcus pneumoniae (77%) and Haemophilus influenzae (73%). Association of nontypeable H influenzae was significantly lower (40%).
CONCLUSIONS: OM is frequently treated by pediatricians in daily practice. A majority of them seem to use antibiotics as first-line treatment. The most common reasons for specialist referrals include treatment failures, recurrent/chronic OM, hearing problems, and surgery. Hearing loss and antibiotic resistance are of concern. Nontypeable H influenzae is less well known as an otopathogen.
RESEARCH OF MANNOSE-BINDING LECTIN AND INTERLEUKINS 10, 12, AND 18 IN CHILDREN WITH HENOCH-SCHÖNLEIN PURPURA
Authors: Li Qiu , She Ping-li , Chen Jia , Zhang Wei 
Published: January 2008

INTRODUCTION: Henoch-Schönlein Purpura (HSP) is a common vasculitis in children, and the unbalance of T-helper 1/T-helper 2 plays an important part in its pathogenesis. Mannose-binding lectin (MBL) is an important component of innate immunity and related with a lot of diseases with immunologic derangement. However, we do not know the relationship between MBL and HSP.
OBJECTIVE: We aimed to explore the serum level and gene polymorphisms of MBL and the levels of interleukin 10 (IL-10), IL-12, and IL-18 in supernatant of peripheral blood mononuclear cells of children with HSP and HSP nephritis (HSPN) and of healthy children.
METHODS: The concentrations of MBL and IL-10, IL-12, and IL-18 were measured by enzyme-linked immunosorbent assay: MBL gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction with sequence-specific primers.
RESULTS: The serum MBL levels in the 23 children with HSP were not significantly different from 27 children with HSPN (P = .95) or 18 normal children. The levels of IL-18 in the supernatant of peripheral blood mononuclear cells in the 3 groups were not significantly different from each other (P = .47, .15, and .14). The levels of IL-10 in children with HSP and HSPN were not different from each other (P = .70), but both were significantly different from those in the normal children (P = .04 and .01). The levels of IL-12 in children with HSP were different from those in the children with HSPN (P = .04). The MBL promoter genotype and the frequency of alleles were not different between the children with HSP and HSPN or between those 2 groups compared with the normal group.
CONCLUSIONS: IL-12 probably plays an important role in the renal involvement in HSP. The position of MBL in the pathogenesis of HSP and HSPN remains to be confirmed.
CHILDREN WITH PERSISTENT WHEEZING ASSOCIATED WITH HUMAN BOCAVIRUS INFECTION IN CHINA
Authors: Enmei Liu , Yu Deng , Xiaodong Zhao , Yuan Ding , Qubei Li , Zhengxiu Luo , Lijia Wang , Xiqiang Yang 
Published: January 2008

INTRODUCTION: Human bocavirus (HBoV) is a newly identified human parvovirus that was originally isolated from children with lower respiratory tract disease. The impact of HBoV on childhood persistent wheezing has not been identified.
OBJECTIVE: Our aim was to study the impact of HBoV on childhood persistent wheezing.
METHODS: In this study, a total of 40 tracheal aspirates were obtained by bronchofibroscope from children with persistent wheezing who had been wheezing for at least &gt;4 weeks. HBoV was detected by polymerase chain reaction. A rapid immunofluorescence method was used for diagnosis of respiratory syncytial virus, adenovirus, influenza A and B, and parainfluenza 1, 2, and 3.
RESULTS: In 40 children with persistent wheezing, 13 (32.5%) had DNA sequences that were HBoV-positive. Age of the patients with HBoV-positive infection ranged from 1 month to 2 years. The results of polymerase chain reaction products sequencing proved that these 13 samples were exactly identical to the sequence of HBoV published in GenBank (accession Nos. DQ988934 and DQ457413). Two children with HBoV infection were found to have coinfection with respiratory syncytial virus.
CONCLUSIONS: This study confirmed that HBoV is a common pathogen for children with lower respiratory infection and might particularly be attributed to persistent wheezing. However, more studies should be performed to study the mechanism of HBoV on chronic airway inflammation.