bluuebunny/crossref_metadata_2025_split huggingface dataset:
Field evaluation of an automated mosquito surveillance system which classifies Aedes and Culex mosquitoes by genus and sex
Authors: González-Pérez María I. , Faulhaber Bastian , Aranda Carles , Williams Mark , Villalonga Pancraç , Silva Manuel , Costa Osório Hugo , Encarnaçao Joao , Talavera Sandra , Busquets Núria 
Published: March 2024

Abstract
Background
Mosquito-borne diseases are a major concern for public and veterinary health authorities, highlighting the importance of effective vector surveillance and control programs. Traditional surveillance methods are labor-intensive and do not provide high temporal resolution, which may hinder a full assessment of the risk of mosquito-borne pathogen transmission. Emerging technologies for automated remote mosquito monitoring have the potential to address these limitations; however, few studies have tested the performance of such systems in the field.

Methods
In the present work, an optical sensor coupled to the entrance of a standard mosquito suction trap was used to record 14,067 mosquito flights of Aedes and Culex genera at four temperature regimes in the laboratory, and the resulting dataset was used to train a machine learning (ML) model. The trap, sensor, and ML model, which form the core of an automated mosquito surveillance system, were tested in the field for two classification purposes: to discriminate Aedes and Culex mosquitoes from other insects that enter the trap and to classify the target mosquitoes by genus and sex. The field performance of the system was assessed using balanced accuracy and regression metrics by comparing the classifications made by the system with those made by the manual inspection of the trap.

Results
The field system discriminated the target mosquitoes (Aedes and Culex genera) with a balanced accuracy of 95.5% and classified the genus and sex of those mosquitoes with a balanced accuracy of 88.8%. An analysis of the daily and seasonal temporal dynamics of Aedes and Culex mosquito populations was also performed using the time-stamped classifications from the system.

Conclusions
This study reports results for automated mosquito genus and sex classification using an optical sensor coupled to a mosquito trap in the field with highly balanced accuracy. The compatibility of the sensor with commercial mosquito traps enables the sensor to be integrated into conventional mosquito surveillance methods to provide accurate automatic monitoring with high temporal resolution of Aedes and Culex mosquitoes, two of the most concerning genera in terms of arbovirus transmission.

Graphical Abstract
Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep
Authors: Niciura Simone Cristina Méo , Cardoso Tainã Figueiredo , Ibelli Adriana Mercia Guaratini , Okino Cintia Hiromi , Andrade Bruno Gabriel , Benavides Magda Vieira , Chagas Ana Carolina de Souza , Esteves Sergio Novita , Minho Alessandro Pelegrine , Regitano Luciana Correia de Almeida , Gondro Cedric 
Published: March 2024

Abstract
Background
The integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological interactions underlying the resistance of hosts to parasites. Haemonchus contortus, the predominant sheep gastrointestinal parasite species in the tropics, causes significant production and economic losses, which are further compounded by the diminishing efficiency of chemical control owing to anthelmintic resistance. Knowledge of how the host responds to infection and how the parasite, in combination with microbiota, modulates host immunity can guide selection decisions to breed animals with improved parasite resistance. This understanding will help refine management practices and advance the development of new therapeutics for long-term helminth control.

Methods
Eggs per gram (EPG) of feces were obtained from Morada Nova sheep subjected to two artificial infections with H. contortus and used as a proxy to select animals with high resistance or susceptibility for transcriptome sequencing (RNA-seq) of the abomasum and 50 K single-nucleotide genotyping. Additionally, RNA-seq data for H. contortus were generated, and amplicon sequence variants (ASV) were obtained using polymerase chain reaction amplification and sequencing of bacterial and archaeal 16S ribosomal RNA genes from sheep feces and rumen content.

Results
The heritability estimate for EPG was 0.12. GAST, GNLY, IL13, MGRN1, FGF14, and RORC genes and transcripts were differentially expressed between resistant and susceptible animals. A genome-wide association study identified regions on chromosomes 2 and 11 that harbor candidate genes for resistance, immune response, body weight, and adaptation. Trans-expression quantitative trait loci were found between significant variants and differentially expressed transcripts. Functional co-expression modules based on sheep genes and ASVs correlated with resistance to H. contortus, showing enrichment in pathways of response to bacteria, immune and inflammatory responses, and hub features of the Christensenellaceae, Bacteroides, and Methanobrevibacter genera; Prevotellaceae family; and Verrucomicrobiota phylum. In H. contortus, some mitochondrial, collagen-, and cuticle-related genes were expressed only in parasites isolated from susceptible sheep.

Conclusions
The present study identified chromosome regions, genes, transcripts, and pathways involved in the elaborate interactions between the sheep host, its gastrointestinal microbiota, and the H. contortus parasite. These findings will assist in the development of animal selection strategies for parasite resistance and interdisciplinary approaches to control H. contortus infection in sheep.

Graphical Abstract
Intrafamily heterooligomerization as an emerging mechanism of methyltransferase regulation
Authors: Hobble Haley V. , Schaner Tooley Christine E. 
Published: March 2024

AbstractProtein and nucleic acid methylation are important biochemical modifications. In addition to their well-established roles in gene regulation, they also regulate cell signaling, metabolism, and translation. Despite this high biological relevance, little is known about the general regulation of methyltransferase function. Methyltransferases are divided into superfamilies based on structural similarities and further classified into smaller families based on sequence/domain/target similarity. While members within superfamilies differ in substrate specificity, their structurally similar active sites indicate a potential for shared modes of regulation. Growing evidence from one superfamily suggests a common regulatory mode may be through heterooligomerization with other family members. Here, we describe examples of methyltransferase regulation through intrafamily heterooligomerization and discuss how this can be exploited for therapeutic use.
Dynamic whole-body [18F]FES PET/CT increases lesion visibility in patients with metastatic breast cancer
Authors: Pedersen Mette A. , Munk Ole L. , Dias André H. , Steffensen Johanne H. , Møller Anders L. , Johnsson Anna Lyhne , Hansen Kim Vang , Bender Dirk , Jakobsen Steen , Busk Morten , Gormsen Lars C. , Tramm Trine , Borgquist Signe , Vendelbo Mikkel H. 
Published: March 2024

Abstract
Background
Correct classification of estrogen receptor (ER) status is essential for prognosis and treatment planning in patients with breast cancer (BC). Therefore, it is recommended to sample tumor tissue from an accessible metastasis. However, ER expression can show intra- and intertumoral heterogeneity. 16α-[18F]fluoroestradiol ([18F]FES) Positron Emission Tomography/Computed Tomography (PET/CT) allows noninvasive whole-body (WB) identification of ER distribution and is usually performed as a single static image 60 min after radiotracer injection. Using dynamic whole-body (D-WB) PET imaging, we examine [18F]FES kinetics and explore whether Patlak parametric images ($${K}_{i}$$

K
i

) are quantitative and improve lesion visibility.

Results
This prospective study included eight patients with metastatic ER-positive BC scanned using a D-WB PET acquisition protocol. The kinetics of [18F]FES were best characterized by the irreversible two-tissue compartment model in tumor lesions and in the majority of organ tissues. $${K}_{i}$$

K
i

values from Patlak parametric images correlated with $${K}_{i}$$

K
i

values from the full kinetic analysis, r2 = 0.77, and with the semiquantitative mean standardized uptake value (SUVmean), r2 = 0.91. Furthermore, parametric $${K}_{i}$$

K
i

images had the highest target-to-background ratio (TBR) in 162/164 metastatic lesions and the highest contrast-to-noise ratio (CNR) in 99/164 lesions compared to conventional SUV images. TBR was 2.45 (95% confidence interval (CI): 2.25–2.68) and CNR 1.17 (95% CI: 1.08–1.26) times higher in $${K}_{i}$$

K
i

images compared to SUV images. These quantitative differences were seen as reduced background activity in the $${K}_{i}$$

K
i

images.

Conclusion
[18F]FES uptake is best described by an irreversible two-tissue compartment model. D-WB [18F]FES PET/CT scans can be used for direct reconstruction of parametric $${K}_{i}$$

K
i

images, with superior lesion visibility and $${K}_{i}$$

K
i

values comparable to $${K}_{i}$$

K
i

values found from full kinetic analyses. This may aid correct ER classification and treatment decisions.
Trial registration ClinicalTrials.gov: NCT04150731, https://clinicaltrials.gov/study/NCT04150731
Contribution of environmental determinants to the risk of developing type 2 diabetes mellitus in a life-course perspective: a systematic review protocol
Authors: Mengue Yannick Wilfried , Audate Pierre-Paul , Dubé Jean , Lebel Alexandre 
Published: March 2024

Abstract
Background
Prevention policies against type 2 diabetes mellitus (T2DM) focus solely on individual healthy lifestyle behaviours, while an increasing body of research recognises the involvement of environmental determinants (ED) (cultural norms of land management and planning, local foodscape, built environment, pollution, and neighbourhood deprivation). Precise knowledge of this relationship is essential to proposing a prevention strategy integrating public health and spatial planning. Unfortunately, issues related to the consistency and synthesis of methods, and results in this field of research limit the development of preventive strategies. This systematic review aims to improve knowledge about the relationship between the risk of developing T2DM in adulthood and long-term exposure to its ED during childhood or teenage years.

Methods
This protocol is presented according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) tools. PubMed, Embase, CINAHL, Web of Science, EBSCO, and grey literature from the Laval University Libraries databases will be used for data collection on main concepts such as ‘type 2 diabetes mellitus’, ‘zoning’ or ‘regional, urban, or rural areas land uses’, ‘local food landscape’, ‘built environment’, ‘pollution’, and ‘deprivation’. The Covidence application will store the collected data for selection and extraction based on the Population Exposure Comparator Outcome and Study design approach (PECOS). Studies published until December 31, 2023, in English or French, used quantitative data about individuals aged 18 and over that report on T2DM, ED (cultural norms of land management and planning, local foodscape, built environment, and neighbourhood deprivation), and their association (involving only risk estimators) will be included. Then, study quality and risk of bias will be conducted according to the combined criteria and ratings from the ROBINS-E (Risk of Bias in Non-randomised Studies—of Exposures) tools and the ‘Effective Public Health Practice Project’ (EPHPP). Finally, the analytical synthesis will be produced using the ‘Synthesis Without Meta-analysis’ (SWiM) guidelines.

Discussion
This systematic review will summarise available evidence on ED associated with T2DM. The results will contribute to improving current knowledge and developing more efficient cross-sectoral interventions in land management and public health in this field of research.

Systematic review registration
PROSPERO CRD42023392073.
The SAFE procedure: a practical stopping heuristic for active learning-based screening in systematic reviews and meta-analyses
Authors: Boetje Josien , van de Schoot Rens 
Published: March 2024

AbstractActive learning has become an increasingly popular method for screening large amounts of data in systematic reviews and meta-analyses. The active learning process continually improves its predictions on the remaining unlabeled records, with the goal of identifying all relevant records as early as possible. However, determining the optimal point at which to stop the active learning process is a challenge. The cost of additional labeling of records by the reviewer must be balanced against the cost of erroneous exclusions. This paper introduces the SAFE procedure, a practical and conservative set of stopping heuristics that offers a clear guideline for determining when to end the active learning process in screening software like ASReview. The eclectic mix of stopping heuristics helps to minimize the risk of missing relevant papers in the screening process. The proposed stopping heuristic balances the costs of continued screening with the risk of missing relevant records, providing a practical solution for reviewers to make informed decisions on when to stop screening. Although active learning can significantly enhance the quality and efficiency of screening, this method may be more applicable to certain types of datasets and problems. Ultimately, the decision to stop the active learning process depends on careful consideration of the trade-off between the costs of additional record labeling against the potential errors of the current model for the specific dataset and context.
Water quality, habitat, and fish assemblage relationships in middle-order agriculture and forest streams of the Mississippi Alluvial Plain
Authors: Skoog Matthew L. , Eggleton Michael A. , Chen Yushun 
Published: March 2024

Abstract
Background
Agriculture has greatly influenced water quality, habitats, and fish assemblages in streams of the Mississippi Alluvial Plain (MAP) ecoregion. However, MAP streams have historically been understudied compared to streams in other agricultural regions of the USA. In this study, water quality, habitat, and fish assemblage composition were assessed seasonally (spring, summer, and fall) in eight representative MAP streams located across three U.S. states. The study design included four streams containing highly agricultural watersheds (herein termed “agriculture” streams) and four streams containing mostly forested watersheds (herein termed “forest” streams), which were intended to represent reference conditions for MAP streams.

Results
In general, forest streams contained significantly better instream and riparian habitats than agriculture streams (P = 0.010–0.040) whereas agriculture streams contained significantly greater levels of primary nutrients (P < 0.001–0.010). Differences between agriculture and forest streams with respect to other physical and chemical variables were intermittent and season dependent. Fish assemblages in agriculture and forest streams were structured primarily along an environmental gradient reflecting instream habitat conditions, water nutrient concentrations, and benthic chlorophyll-a production. Structurally, fish assemblages in both stream types contained many regionally common species, though some species appeared to exhibit affinities for a particular stream type. Functionally, fish assemblages in agriculture streams contained more tolerant species, more omnivores, and fewer insectivores compared to forest stream assemblages, which were nearly all insectivores. Overall, one-third of the fish specimens collected in forest streams classified as intolerant species.

Conclusions
Our results suggested that stream water quality, habitat, and fish assemblages differed between agriculture and forest streams in the MAP, with fish assemblages exhibiting both structural and functional differences. Results were consistent with a larger body of literature from smaller, headwater streams whereby land-use changes (e.g., row-crop agriculture) impacted the physical, chemical, and biological characteristics of stream ecosystems. Results further highlight the importance of land use management and its effects on habitat diversity in stream ecosystems, and that protecting the few remaining undisturbed or less-disturbed streams should be a priority.
Clinical evidence of the link between gut microbiome and myalgic encephalomyelitis/chronic fatigue syndrome: a retrospective review
Authors: Wang Jing-Hua , Choi Yujin , Lee Jin-Seok , Hwang Seung-Ju , Gu Jiyeon , Son Chang-Gue 
Published: March 2024

AbstractMyalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a heterogeneous disorder with elusive causes, but most likely because of clinical and other biological factors. As a vital environmental factor, the gut microbiome is increasingly emphasized in various refractory diseases including ME/CFS. The present study is aimed to enhance our understanding of the relationship between the gut microbiome and ME/CFS through data analysis of various clinical studies. We conducted a literature search in four databases (PubMed, Cochrane Library, Web of Science, and Google Scholar) until May 31, 2023. Our analysis encompassed 11 clinical studies with 553 ME/CFS patients and 480 healthy controls. A comparative analysis of meta data revealed a significant decrease in α-diversity and a noticeable change in β-diversity in the gut microbiome of ME/CFS patients compared to healthy controls. The notable ratio of Firmicutes and Bacteroides was 2.3 times decreased, and also, there was a significant reduction in the production of microbial metabolites such as acetate, butyrate, isobutyrate, and some amino acids (alanine, serine, and hypoxanthine) observed in ME/CFS patients. The lack of comparison under similar conditions with various standardized analytical methods has impeded the optimal calculation of results in ME/CFS patients and healthy controls. This review provides a comprehensive overview of the recent advancements in understanding the role of the gut microbiome in ME/CFS patients. Additionally, we have also discussed the potentials of using microbiome-related interventions and associated challenges to alleviate ME/CFS.
Residual calcified material volume of β–tricalcium phosphate with platelet-rich fibrin in unilateral alveolar bone graft
Authors: Ho Nguyen Chon T. , Bui Minh H. , Lam Phuong H. 
Published: March 2024

Abstract
Background
This study aimed to evaluate the effectiveness of β-tricalcium phosphate (β-TCP) and platelet-rich fibrin (PRF) in unilateral alveolar bone graft, involving the percentage of residual calcified material and the average labiopalatal thickness of the grafts on cone beam computed tomography at 6 months after surgery, comparing two age groups 12 years and under and over 12 years old.

Results
The mean preoperative defect volume was 0.93 ± 0.20 cm3, with no significant difference between the two groups (p = 0.652). In the postoperative period, we did not record any abnormal bleeding and no infection was observed. Six months after surgery, the mean percentage of residual calcified material was 63.53 ± 16.48% with a significantly higher difference in the age group 12 and under (p < 0.001), and the mean average labiopalatal thickness of the grafted bone was 5.72 ± 1.09 mm with a significantly higher difference in the age group 12 and under (p = 0.011).

Conclusion
Using β-TCP and PRF in alveolar bone graft surgery has acceptable effectiveness clinically and on CBCT images, with significantly higher differences of the percentage of residual calcified material and the average labiopalatal thickness of the grafted bone in the group 12 years old and younger than in the older group.
Audiologists’ practice and perspectives on rehabilitation in individuals with minimal-mild degree of hearing loss: a questionnaire-based survey
Authors: Goyal Swati , E. Prajwal Kumar , Dutta Kristi Kaveri , S. Gowtham Varma , Thomas Prajeesh 
Published: March 2024

Abstract
Background
The study aimed to understand the perspectives of audiologists working in different audiological setups regarding the fitting of amplification devices for individuals with minimal-mild hearing loss which cannot be corrected or sensorineural in nature. The study employed a questionnaire-based survey research design conducted in three phases: questionnaire development, obtaining responses from audiologists, and analyzing the responses. The questionnaire consisted of three sections: demographic details, audiologist's practice, and audiologist's opinion, with a total of 22 questions. The responses from audiologists were collected through an online survey distributed via WhatsApp groups, email, and Telegram. Descriptive statistics were used to analyze the percentage of responses for each question.

Results
The findings shed light on the current practices and opinions of audiologists. The study found that audiologists perceived the assessment of self-handicap level as aiding in better counseling, understanding motivation level, and making decisions about amplification.

Conclusions
The findings of the study provide insights into the current practices and perspectives of audiologists in offering services to individuals with minimal-mild hearing loss.
Two-staged hybrid repair of multiple great vessel and thoracic aneurysms with right vocal fold palsy using retrograde in situ branched stent grafting: a case report
Authors: Imamura Yuki , Saito Yoshiaki , Murata Kenyu , Itokawa Rin , Taguchi Ryo , Minakawa Masahito 
Published: March 2024

Abstract
Background
Repair of multiple thoracic aneurysms with vocal fold palsy represents a surgical challenge. Here, we present a case of multiple cervical and thoracic aortic aneurysms with right vocal fold palsy successfully treated with two-stage hybrid repair using retrograde in situ branched grafting.

Case presentation
A 52-year-old patient with multiple cervical and thoracic aortic aneurysms and right vocal fold palsy was treated using two-stage hybrid repair. Initially, the patient underwent an open partial arch replacement with reconstruction of the right subclavian, right common carotid, and left common carotid arteries. Three months after the initial procedure, the left subclavian artery was reconstructed during thoracic endovascular aortic repair with retrograde in situ stent grafting using the needle technique. The postoperative course was uneventful with no endoleaks detected using postoperative computed tomography.

Conclusion
This case highlighted the potential of two-stage repair of multiple thoracic aneurysms using retrograde in situ stent grafting. With the expansion of surgical options to include endovascular and hybrid approaches, surgical procedures should be carefully planned for individuals with complex aortic diseases.