bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics of non-tuberculous mycobacterium in central China were analyzed based on the macropathogenic mNGS sequencing technology
Authors: Yang Feicheng , Liu Yu , Yang Yuzhong , Chen Si , Liu Xiaoying , Dai Penghui , Yang Zhou , Zhou Yan 
Published: March 2024

Abstract
Objective: Metagenomics next generation sequencing (mNGS) was used to investigate the pathogenic factors, epidemic characteristics and clinical characteristics of non-tuberculous mycobacterium in central China.
Methods：The pathological data of 320 patients with mycobacterium infection diagnosed in the Department of Pathology of Hunan People's Hospital from June 2019 to December 2023 were collected, including HE staining, acid-fast and fungal fluorescence staining, and macrogenetic pathoomics next generation sequencing (mNGS) detection to classify mycobacterium pathogens.
Results：Among 320 patients with mycobacterium detection, 249 were positive for mycobacterium tuberculosis and 71 were positive for non-mycobacterium tuberculosis. Thirteen non-tuberculous mycobacterium genera were identified in the samples from central China, including M. chelonis, M. avium complex group, M. abscess, M. Gordon, M. intracellular, M. tobacco-Kansas, M. paracondontium, M. Aubris, M. margherita, M. fortuitus, M. smegmatis and M. margherita.
Conclusion:Non-tuberculous mycobacterium infections gradually increased, and the clinical infection mode began to change from single strain infection to multi-strain combined complex infection. It was very difficult to distinguish non-tuberculous mycobacterium from mycobacterium tuberculosis by simple pathologic diagnosis. The application of mNGS contributed to solving this problem and improving the clinical diagnosis rate and early treatment.
Investigating Snoring Patterns and Characteristics among Residents in the Qingpu District
Authors: Li Yan , Zhang Qi , Wang Shengli , Bai Guangping 
Published: March 2024

Abstract
Objective: The primary objective of this research was to conduct polysomnography tests on individuals diagnosed with Obstructive Sleep Apnea-Hypopnea Syndrome (OSAHS) in the Qingpu suburb of Shanghai, China. The study entailed the examination of diverse sleep monitoring parameters linked to age, gender, and disease severity to thoroughly investigate snoring patterns among residents of the Qingpu District. The resulting research outcomes are intended to offer significant insights for formulating successful clinical treatment plans and strategies. Methods: Polysomnography was conducted on 90 snoring patients, and parameters including Apnea-Hypopnea Index (AHI), Oxygen Desaturation Index (ODI), Snoring Index (SI), AGE, GENDER, and Body Mass Index (BMI) were statistically evaluated. Results: Average values of AHI, ODI, SI, and BMI across different age groups were relatively high without significant variations. When examining gender differences, it was found that both men and women exhibited higher average Apnea-Hypopnea Index (AHI) and Oxygen Desaturation Index (ODI), while notable discrepancies were not observed in Snore Index (SI), age, or Body Mass Index (BMI) between men and women.When stratified by the severity of OSAHS, box plots for AHI and ODI revealed a progressive rise in average values as OSAHS severity increased, culminating at severe stages. The correlation analyses indicated a robust positive relationship between AHI and ODI, with correlation coefficients r values of 0.30, 0.55, 0.77, and 0.84 for normal, mild, moderate, and severe OSAHS categories, respectively. Conclusion: Age did not significantly impact the mean values of AHI, ODI, SI, and BMI in residents of Qingpu, Shanghai. No substantial gender disparities were observed concerning snoring index, age of onset, and BMI. However, men exhibited a higher propensity for apnea and hypoxia compared to women, particularly in terms of AHI and ODI. The study highlighted a robust association between AHI and ODI, with the strength of this association increasing with OSAHS severity levels.
Quality characteristics and thermal behavior diversity of traditional crude shea (Vitellaria paradoxa Gaertn) butter from Burkina Faso
Authors: Goumbri Bertrand W. F. , Djang’eing’a Roland Marini , Semdé Rasmané , Mouithys-Mickalad Ange , Sakira Abdoul Karim , Yaméogo Gérard B. Josias , Somé Touridomon Issa , Danthine Sabine 
Published: March 2024

Abstract

The aim of this study was to establish a comparative analysis of the physicochemical and the thermal behavior (solid fat content (SFC) by p-NMR, and by differential scanning calorimetry (DSC) of shea butter (SB) from Burkina Faso, related to different traditional extraction processes. Thirty-seven samples obtained from different localities were collected from local producers and analyzed for chemical indexes, unsaponifiable matter content (UMC), color, yellow index, fatty acids (FA) profile, and thermal behavior. Results showed that stearic acid and oleic acid were the most important FA but varied from 47.2 ± 0.2 to 51.0 ± 0.1%, respectively. Chemical quality variations among the 37 samples were observed: UMC (3.0 ± 1.0 to 12.0 ± 0.1%), peroxide value (4.6 ± 0.1 to 44.5 ± 0.2 meqO2/kg), iodine value by GC (50.5 to 64.2 g/100g), free fatty acids (1.1 ± 0.1 to 9.7 ± 0.1%). Statistically significant relationships were observed between thermal properties and chemical parameters. The results were subjected to clustering and principal component analysis (PCA). Results showed that a great diversity exists among crude shea butter samples from Burkina Faso which is due to both regional variation and difference in extraction processes. A standardization of the process could help to improve the quality.
A fluorescent probe for Hg 2+ specific recognition based on xanthene and its application in food detection and cell imaging
Authors: Zhang Chenglu , Nie Shiru , Liu Chang , zhang Yang , Guo Jinghao 
Published: March 2024

Abstract
The mercury-loving unit aminothiourea was introduced into the xanthene fluorophore to synthesized the probe molecule NXH. NXH has a specific response to Hg2+, and with the addition of (0 ~ 50 µM) Hg2+, the fluorescence intensity of the probe solution was quenched from 2352 a.u. to about 308 a.u.. The probe NXH exhibited excellent detection performance of high sensitivity (LOD = 96.3 nM), real-time response (105 s), wide pH range (2.1 ~ 9.3), and strong anti-interference ability for Hg2+. At the same time, the probe NXH has wide range of applications for Hg2+ detection, which can be used to create molecular logic gates, make Hg2+ detection test paper, as well as the fluorescence imaging of Hg2+ in Hela live cells and tea samples.
Comparative Analysis of Machine Learning Algorithms for Predicting House Prices
Authors: Yamannage Sachith Nimesh 
Published: March 2024

Abstract
This study conducted a thorough examination of residential property data in the Abbotsford area of M elbourne with the goal of identifying significant trends in housing, regional patterns, and variables aff ecting home values.The dataset contained a number of elements, such as neighborhood information, g eographic coordinates, transaction details, and property attributes.Using a variety of techniques, inclu ding mean imputation, forward filled imputation, machine learning algorithms, and discarding missin g data, the study started with the identification and treatment of missing values.Particularly in the pric e variable, outliers were found, and boxplots and other visualization tools were used for outlier analysi s.For additional analysis, numerical values representing the categorical variables were converted.To in vestigate the distributions of numerical variables and comprehend connections between variables with a focus on correlations with home prices—univariate and bivariate analyses were carried out. Feature engineering, covariance analysis, ANOVA testing, and predictive modeling with regression algorithms like Random Forest, XGBoost, and Support Vector Machine (SVM) were all part of the quantitative analysis process. Metrics like Mean Absolute Error (MAE) were used to assess the performance of the model; the results showed that XGBoost was the most accurate predictor of housing prices. Significant factors influencing home prices were identified by the study, such as building area, property type, number of rooms, and geographic considerations including proximity to important sites. Each component was analyzed in terms of its relative relevance, and the building area and land size. It was noted how the constructed model has limits, such as overfitting and the need for more model refining. The results offer insightful information to scholars, politicians, and real estate professionals who are interested in the dynamics of the housing market.
High-sensitivity whole-mount in situ hybridization of mouse oocytes and embryos visualizes the super-resolution structures and distributions of mRNA molecules
Authors: Sanada Takahiro , Kotani Tomoya 
Published: March 2024

Abstract
Background
Mammalian oocytes accumulate more than ten thousand mRNAs, of which three to four thousand mRNAs are translationally repressed. The timings and sites of translational activation of these dormant mRNAs are crucial for promoting oocyte maturation and embryonic development. How these mRNAs are accumulated and distributed in oocytes is therefore a fundamental issue to be explored. A method that enables visualization of mRNA molecules with high resolution in a simple manner would be valuable for understanding how oocytes accumulate and regulate the dormant mRNAs.
Results
We have developed a highly sensitive whole-mount in situ hybridization method using in vitro-synthesized RNA probes and the tyramide signal amplification (TSA) system optimized for mouse oocytes and embryos. Using this method, Pou5f1/Oct4, Emi2, and cyclin B1 mRNAs were detected in immature oocytes and 2-cell stage embryos. Confocal microscopy showed that these mRNAs formed granular structures in the oocyte cytoplasm. The structures of Pou5f1/Oct4 and cyclin B1 mRNAs persisted in 2-cell stage embryos. Pou5f1/Oct4 RNA granules exhibited a solid-like property in immature oocytes and became liquid-like droplets in 2-cell stage embryos. Double-staining of cyclin B1 mRNA with Emi2 or Pou5f1/Oct4 mRNA revealed that these mRNAs were distributed as different RNA granules without overlapping each other and that the size of cyclin B1 RNA granules tended to be larger than that of Emi2 RNA granules. The structures and distribution patterns of these mRNAs were further analyzed by N-SIM super-resolution microscopy. This analysis revealed that the large-sized RNA granules consist of many small-sized granules, suggesting the accumulation and regulation of dormant mRNAs as basal-sized RNA granules.
Conclusion
This method can easily visualize the structure and distribution of mRNAs accumulated in mammalian oocytes and embryos with high sensitivity and super-resolution. This method is useful for investigating the cellular and molecular mechanisms of translational control of mRNAs by which maturation and early developmental processes are promoted.
Association between class III obesity and overall survival in previously untreated younger patients with acute myeloid leukemia enrolled on SWOG S1203
Authors: Percival Mary-Elizabeth , Zhang Michelle , Othus Megan , McMillen Kerry , Erba Harry , Garcia-Manero Guillermo , Pagel John , Sorror Mohamed 
Published: March 2024

Abstract

There has been ongoing debate on the association between obesity and outcomes in acute myeloid leukemia (AML). Currently there are few studies that have stratified outcomes by class I obesity, class II obesity, and class III obesity; and a more nuanced understanding is becoming increasingly important with the rising prevalence of obesity. We examined the association between body mass index (BMI) and outcomes in previously untreated AML in younger patients (age ≤60) enrolled in SWOG S1203 (n=729). Class III obesity was associated with an increased rate of early death (p=0.004) and worse overall survival (OS) in multivariate analysis (hazard ratio (HR) 2.48, 95% confidence interval (CI) 1.62-3.80 versus normal weight). Class III obesity was also associated with worse OS after allogeneic hematopoietic cell transplant (HR 2.37, 95% CI 1.24-4.54 versus normal weight). These findings highlight the unique risk of class III obesity in AML, and the importance of further investigation to better characterize this patient population.
Photosynthetic Physiological Regulation Mechanism of VIRESCENT-ALBINO LEAF 1 in Rice (Oryza sativa L.)
Authors: Li Lingyi , Yang Shihui , Jiao Yingrui , Liu Yufei , Wang Bingbing , Zhang Hongmei , Hu Jian , Wang Guangyuan , Gul Shareef , Feng Ping , Zhang Ting , Ling Yinghua , He Guanghua , Yao Hesheng 
Published: March 2024

Abstract
Improving photosynthetic potential and light use efficiency is a crucial way to increase rice yield. The virescent-albino leaf 1 (val1) mutant, wild-type ‘Jinhui 10’ (WT), and VAL1 gene overexpression plants (VAL1-OE) were used to determine the physiological mechanisms of survival strategy in val1 mutant and improvement of photosynthetic potential in VAL1-OE through investigating leaf photosynthetic characteristics, photoprotection processes and yield. The results showed that, compared with WT, the expressions of key genes in photosynthetic pathway and chlorophyll contents in val1 mutant were both significantly lower at the early growth stage and higher at the late growth stage. Besides, the photosynthetic electron transport rate, the quantum yield of photosystem II and I, and carboxylation efficiency in val1 mutant were significantly lower than those in WT at the early growth stage, but significantly higher at the late growth stage. Furthermore, the non-photochemical quenching (NPQ) of val1 mutant was significantly higher than WT, thereby optimizing the heat dissipation pathway on the basis of reducing the chlorophyll contents and light absorption at the early growth stage. The NPQ and cyclic electron flow (CEF) were significantly higher in val1 mutant than WT at the late growth stage, resulting in the improvements of light use efficiency and photosynthetic acclimation under both low and high light conditions. Compared with WT, the expressions of key genes in photosynthetic pathway and chlorophyll contents were significantly higher in VAL1-OE. The photosynthetic electron transport rate, quantum yield of photosystem II and I, and the carboxylation efficiency in VAL1-OE were significantly higher than those in WT, improving light use efficiency and carboxylation efficiency of VAL1-OE. Overall, the val1 mutant survived by the optimal plant phenotype and leaf photoprotection pathway at the early growth stage, and improved photosynthetic potential and light use efficiency at the late growth stage. VAL1-OE improved light absorption by optimizing the dissipation pathway of excess light energy so as to increase light use efficiency and carboxylation efficiency. Cultivating phenotypic materials with high leaf area on the basis of high photosynthesis rate in VAL1-OE could be a breakthrough in high photosynthetic efficiency rice breeding.
Overexpression of COL4A1 promotes progression of colon cancer and is associated with poor prognosis
Authors: Li Qiusheng , Wang Changjing , Wang guiying 
Published: March 2024

Abstract
Background
Colon cancer is one of the common malignant tumor diseases of the digestive system, and its incidence and mortality rate are increasing year by year.Collagen type IV alpha 1 chain (COL4A1) is a hypoxia-associated gene (a collagen family protein) involved in the formation of the extracellular matrix.COL41 plays an important role in the progression of several cancers and is associated with poorer prognostic outcomes.However, whether it can mediate the progression of colon cancer is unclear.
Method
First, based on the TCGA-COAD dataset, we analyzed the mRNA expression levels of COL4A1 in colon cancer and evaluated the relationship between COL4A1 and clinicopathological features as well as prognosis.Then,we further analyzed the expression of COL4A1 in colon cancer tissues and colon tumor cells by qRT-PCR and HIC.Colon cancer cell lines SW480 and HCT116 were transfected with small interfering rna (siRNA) to construct low expression cell lines.Clone formation assay and transwell migration and invasion assays were used to detect the effects of knockdown of COL4A1 on proliferation, migration and invasion of colon cancer cells.
Result
Bioinformatics analysis showed that COL4A1 was significantly higher expressed in colon cancer tissues than in normal colon tissues. High expression of COL4A1 is associated with poor clinical stage and poor prognosis.We observed that COL4A1 was also highly expressed in colon cancer tissue and colon tumor cells compared with normal colon tissue and normal colon cells.Downregulation of COL4A1 significantly inhibited tumor cell proliferation, migration and invasion progression.
Conclusion
In summary, our study suggests that COL4A1 is a potential oncogene for colon cancer and can mediate colon cancer progression.
PPM1D/Wip1 is amplified, overexpressed, and mutated in human Non-Hodgkin Lymphomas
Authors: Pilevneli Hatice , Doger Firuzan , Karagenc Levent , Kozaci Leyla Didem , Eren Mehtap Kilic 
Published: March 2024

Abstract

Background Wip1, is a p53-dependent Ser/Thr phosphatase involved in the timely termination of DDR. ThePPM1Dgene encoding Wip1 is deregulated and thus gained an oncogene character in common human solid tumors and cell lines. This study assessed the oncogenic potential of thePPM1Dgene in human NHL, the most common hematological malignancy worldwide. Methods and Results FFPE human LH (n = 17) and NHL tumor lymph node samples (n = 65) and human NHL cell lines were used to assess the oncogenic potential of thePPM1Dgene in the present study. Copy number gain and mRNA expression analysis of thePPM1D/Wip1 gene were assessed by qRT-PCR analysis. Mutational analysis of Exon 6 of thePPM1Dgene was performed by PCR amplification and Sanger sequencing. Expressions of Wip1 and p53 proteins were assessed by immunohistochemistry and western blot analysis. Conclusions We found thatPPM1Dgained gene copy number in NHL tumors by 0.7-8 times compared to the control (p < 0.01). IncreasedPPM1D/Wip1 gene copy number was associated with higher mRNA and protein expression in human NHL samples (p < 0.01). Overexpression of Wip1 in NHL tumors and NHL cell lines was associated with amplification level and was unaffected by p53 status. Furthermore, a heterozygous type mutation was detected in exon 6 (c.1553C > A, p.518) of thePPM1Dgene particularly in DLBCL samples. Wip1 may have oncogenic potential, perhaps playing a role in the onset and progression of human NHL. The possible significance of Wip1 overexpression to chemotherapy response in NHL remains an intriguing question that requires more exploration.
Infection episodes and islet autoantibodies in children at increased risk for type 1 diabetes before and during the COVID-19 pandemic
Authors: Zeller Ivo , Weiss Andreas , Arnolds Stefanie , Schütte-Borkovec Katharina , Arabi Sari , Berge Thekla , Casteels Kristina , Hommel Angela , Kordonouri Olga , Larsson Helena Elding , Lundgren Markus , Rochtus Anne , Snape Matthew D , Szypowka Agnieszka , Vatish Manu , Winkler Christiane , Bonifacio Ezio , Ziegler Anette-Gabriele 
Published: March 2024

Abstract

Purpose. To determine the impact of the COVID-19 pandemic on the incidence rates of infection and islet autoimmunity in children at risk for type 1 diabetes. Methods. 1050 children aged 4 to 7 months with an elevated genetic risk for type 1 diabetes were recruited from Germany, Poland, Sweden, Belgium and the UK. Reported infection episodes and islet autoantibody development were monitored until age 40 months from February 2018 to February 2023. Results. The overall infection rate was 311 (95% Confidence Interval [CI], 304–318) per 100 person years. Infection rates differed by age, country, family history of type 1 diabetes, and period relative to the pandemic. Total infection rates were 321 per 100 person-years (95% CI, 304–338) in the pre-pandemic period (until February 2020), 160 (95% CI, 148–173) per 100 person-years in the first pandemic year (March 2020 - February 2021; P < 0.001) and 337 (95% CI, 315–363) per 100 person-years in subsequent years. Similar trends were observed for respiratory and gastrointestinal infections. Islet autoantibody incidence rates were 1.6 (95% CI, 1.0-2.4) per 100 person-years in the pre-pandemic period, 1.2 (95% CI, 0.8–1.9) per 100 person-years in the first pandemic year (P = 0.46), and 3.4 (95% CI, 2.3–4.8) per 100 person-years in subsequent years (P = 0.005 vs. pre-pandemic year; P < 0.001 vs. first pandemic year). Conclusions. The COVID-19 pandemic significantly altered infection patterns. Islet autoantibody incidence rates increased two-fold when infection rates returned to pre-pandemic levels.
Histo-morphological changes induced by nano nickel particles in rat testis
Authors: Singh Meenu , Verma Yeshvandra , Rana S V S 
Published: March 2024

Abstract
Exposure to nickel nanoparticles is known to cause adverse health effects in man and animal models. Present investigations were undertaken to determine the histo-morphological changes induced by nickel nanoparticles in testis of Wistar rats with special reference to duration of exposure. Suitable numbers of rats were fed with NiONPs and NiOMPs (5mg/kg b.w.each) for 15 and 30 days. Testes were removed and processed for histopathological, histochemical and SEM observations. Bioconcentration of nickel in testis and serum level of testosterone were also estimated. The results showed that NiONPs and NiOMPs could induce specific time dependent lesions in the testis of rat. Degeneration of germinal epithelium, spermatocytes, hypertrophy of seminiferous tubules and necrosis were observed. Localization of alkaline phosphatase was also altered. SEM results also indicated specific morphological changes in cellular components of tubules. In conclusion, present study suggests that testis is a target organ of NiONPs toxicity where its exposure time dependent effects are expressed. The results will be helpful in understanding the reproductive toxicity of new materials.