bluuebunny/crossref_metadata_2025_split huggingface dataset:
The onset of Neo-Tethys subduction in the Early Jurassic: evidence from the eclogites of the North Shahrekord Metamorphic Complex (Sanandaj-Sirjan Zone, W Iran)
Authors: Montemagni Chiara , Zanchetta Stefano , Malaspina Nadia , Javadi Hamid Reza , Zanchi Andrea 
Published: April 2024

AbstractGeodynamic models implying subduction of continental crust either consider this process happening during collision, when the continental margin of the lower plate attempts subduction, or in pre-collisional stages, when tectonic erosion of the upper plate or subduction of continental extensional allochthons drag continental crust in the subduction channel. In the Zagros orogen (W Iran), high-pressure rocks are known only from the Sanandaj-Sirjan Zone, NE of the Main Zagros Thrust. Here, eclogites of the North Shahrekord Metamorphic Complex suggest subduction of continental crust slices derived from the upper plate (Central Iran) during the onset of the Neo-Tethys subduction along the southern margin of Iran. Eclogites record a clockwise pressure-temperature-time path, with pre-eclogitic epidote-amphibolites-facies phase assemblages preserved in garnet cores, a high-pressure stage, and a subsequent retrogression at amphibolite-facies conditions. By means of forward thermodynamic modelling and 40Ar/39Ar geochronology, the peak metamorphism has been constrained at 1.9-2.1 GPa and 550-600 °C, in the 191-194 Ma time span. The following retrogression during exhumation lasted at least until 144 Ma. Our data suggest that the onset of the Neo-Tethys subduction traces back prior to 190 Ma, involving together with the Neo-Tethys oceanic lithosphere also slices of the upper plate continental crust scraped off by means of tectonic erosion processes.
Statistical analysis of species association indices
Authors: Mendoza Manuel , Mendoza Eduardo , Gutiérrez-Peña E. 
Published: April 2024

Abstract
The study of species association is of great interest in ecology due to its role in understanding key issues such as patterns of habitat use by animals, species coexistence, biotic interactions, and in general factors affecting community structure and assembly. There are many indices that ecologists commonly use, all based on the observed frequencies of organism occurrences, to evaluate the association between a pair of species. However, few of these indices correspond to proper statistical measures of association, and the inferential aspects of their analysis are often overlooked. In this paper, we propose a Bayesian approach based on a simple multinomial-Dirichlet structure to provide a comprehensive inferential framework for any set of association indices. Our approach provides a full statistical analysis for any association index of interest, free of special requirements on the sample size. We illustrate our procedure with a camera-trapping real-dataset, but the analysis of any other dataset of the same type can be readily produced using the R package basa that accompanies this paper.
Method and Validation of Real‐Time Global Ionosphere Modeling Constraint by Multi‐Source GNSS/LEO Data
Authors: Chen Jun , Ren Xiaodong , Xu Guozhen , Yang Pengxin , Liu Hang , Zhang Xiaohong 
Published: April 2024

AbstractThis study applies the zero‐differenced integer ambiguity method, named PPP‐Fixed, to extract real‐time ionospheric data and eliminate the latencies of rapid/final Global Ionosphere Maps (GIMs). The PPP‐Fixed method is also used to derive ionospheric data for post‐processed GIM generation, named SGG Post‐GIM, combined with low earth orbit satellite data. The obtained hardware delays are applied to revise real‐time ionospheric data. Meanwhile, the estimated multi‐source ionospheric model is regarded as historical data to estimate an ionospheric prediction model for constraint using the semi‐parameter model. Then, the Kalman filter is employed to estimate the parameters to generate real‐time GIM. Finally, the accuracy of estimated real‐time GIM, named SGG RT‐GIM, and SGG Post‐GIM is assessed. During the experimental period, the mean differences of SGG Post‐GIM and SGG RT‐GIM relative to GIMs provided by the international Global Navigation Satellite System service, named IGSG, are −0.46 and −0.57 Total Electron Content Unit (TECU), respectively. The corresponding Root Mean Square (RMS) values are 1.64 and 3.08 TECU. Over the test period, the mean positioning errors of the single‐frequency precise point positioning corrected by IGSG, SGG Post‐GIM, SGG RT‐GIM, and Klobuchar model are 0.14, 0.19, 0.21, and 0.25 m in the horizontal direction, respectively, while the corresponding errors are 0.36, 0.33, 0.38, and 0.64 m in the up direction. Further, the mean biases of experimental days for the self‐consistency assessment are 0.06, −0.01, and −0.07 TECU for IGSG, SGG Post‐GIM, and SGG RT‐GIM, respectively. The corresponding RMS values are 1.19, 1.15, and 1.57 TECU.
PGC-1a mediated mitochondrial biogenesis promotes recovery and survival of neuronal cells from cellular degeneration
Authors: You Wenting , Knoops Kèvin , Berendschot Tos T. J. M. , Benedikter Birke J. , Webers Carroll A. B. , Reutelingsperger Chris P. M. , Gorgels Theo G. M. F. 
Published: April 2024

AbstractNeurodegenerative disorders are characterized by the progressive loss of structure and function of neurons, often including the death of the neuron. Previously, we reported that, by removing the cell death stimulus, dying/injured neurons could survive and recover from the process of regulated cell death, even if the cells already displayed various signs of cellular damage. Now we investigated the role of mitochondrial dynamics (fission/fusion, biogenesis, mitophagy) in both degeneration and in recovery of neuronal cells. In neuronal PC12 cells, exposure to ethanol (EtOH) induced massive neurite loss along with widespread mitochondrial fragmentation, mitochondrial membrane potential loss, reduced ATP production, and decreased total mitochondrial volume. By removing EtOH timely all these mitochondrial parameters recovered to normal levels. Meanwhile, cells regrew neurites and survived. Study of the mitochondrial dynamics showed that autophagy was activated only during the cellular degeneration phase (EtOH treatment) but not in the recovery phase (EtOH removed), and it was not dependent on the Parkin/PINK1 mediated mitophagy pathway. Protein expression of key regulators of mitochondrial fission, phospho-Drp1Ser616and S-OPA1, increased during EtOH treatment and recovered to normal levels after removing EtOH. In addition, the critical role of PGC-1α mediated mitochondrial biogenesis in cellular recovery was revealed: inhibition of PGC-1α using SR-18292 after EtOH removal significantly impeded recovery of mitochondrial damage, regeneration of neurites, and cell survival in a concentration-dependent manner. Taken together, our study showed reversibility of mitochondrial morphological and functional damage in stressed neuronal cells and revealed that PGC-1α mediated mitochondrial biogenesis played a critical role in the cellular recovery. This molecular mechanism could be a target for neuroprotection and neurorescue in neurodegenerative diseases.
An enzymatic continuous-flow reactor based on a pore-size matching nano- and isoporous block copolymer membrane
Authors: Zhang Zhenzhen , Gao Liang , Boes Alexander , Bajer Barbara , Stotz Johanna , Apitius Lina , Jakob Felix , Schneider Erik S. , Sperling Evgeni , Held Martin , Emmler Thomas , Schwaneberg Ulrich , Abetz Volker 
Published: April 2024

AbstractContinuous-flow biocatalysis utilizing immobilized enzymes emerged as a sustainable route for chemical synthesis. However, inadequate biocatalytic efficiency from current flow reactors, caused by non-productive enzyme immobilization or enzyme-carrier mismatches in size, hampers its widespread application. Here, we demonstrate a general-applicable and robust approach for the fabrication of a high-performance enzymatic continuous-flow reactor via integrating well-designed scalable isoporous block copolymer (BCP) membranes as carriers with an oriented and productive immobilization employing material binding peptides (MBP). Densely packed uniform enzyme-matched nanochannels of well-designed BCP membranes endow the desired nanoconfined environments towards a productive immobilized phytase. Tuning nanochannel properties can further regulate the complex reaction process and fortify the catalytic performance. The synergistic design of enzyme-matched carriers and efficient enzyme immobilization empowers an excellent catalytic performance with >1 month operational stability, superior productivity, and a high space-time yield (1.05 × 105 g L−1 d−1) via a single-pass continuous-flow process. The obtained performance makes the designed nano- and isoporous block copolymer membrane reactor highly attractive for industrial applications.
Cross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topologies
Authors: Zhu Ying , Akkaya Kerem Can , Ruta Julia , Yokoyama Nanako , Wang Cong , Ruwolt Max , Lima Diogo Borges , Lehmann Martin , Liu Fan 
Published: April 2024

AbstractThe functions of cellular organelles and sub-compartments depend on their protein content, which can be characterized by spatial proteomics approaches. However, many spatial proteomics methods are limited in their ability to resolve organellar sub-compartments, profile multiple sub-compartments in parallel, and/or characterize membrane-associated proteomes. Here, we develop a cross-link assisted spatial proteomics (CLASP) strategy that addresses these shortcomings. Using human mitochondria as a model system, we show that CLASP can elucidate spatial proteomes of all mitochondrial sub-compartments and provide topological insight into the mitochondrial membrane proteome. Biochemical and imaging-based follow-up studies confirm that CLASP allows discovering mitochondria-associated proteins and revising previous protein sub-compartment localization and membrane topology data. We also validate the CLASP concept in synaptic vesicles, demonstrating its applicability to different sub-cellular compartments. This study extends the scope of cross-linking mass spectrometry beyond protein structure and interaction analysis towards spatial proteomics, and establishes a method for concomitant profiling of sub-organelle and membrane proteomes.
Improvement of immune dysregulation in individuals with long COVID at 24-months following SARS-CoV-2 infection
Authors: Phetsouphanh Chansavath , Jacka Brendan , Ballouz Sara , Jackson Katherine J. L. , Wilson Daniel B. , Manandhar Bikash , Klemm Vera , Tan Hyon-Xhi , Wheatley Adam , Aggarwal Anupriya , Akerman Anouschka , Milogiannakis Vanessa , Starr Mitchell , Cunningham Phillip , Turville Stuart G. , Kent Stephen J. , Byrne Anthony , Brew Bruce J. , Darley David R. , Dore Gregory J. , Kelleher Anthony D. , Matthews Gail V. 
Published: April 2024

AbstractThis study investigates the humoral and cellular immune responses and health-related quality of life measures in individuals with mild to moderate long COVID (LC) compared to age and gender matched recovered COVID-19 controls (MC) over 24 months. LC participants show elevated nucleocapsid IgG levels at 3 months, and higher neutralizing capacity up to 8 months post-infection. Increased spike-specific and nucleocapsid-specific CD4+ T cells, PD-1, and TIM-3 expression on CD4+ and CD8+ T cells were observed at 3 and 8 months, but these differences do not persist at 24 months. Some LC participants had detectable IFN-γ and IFN-β, that was attributed to reinfection and antigen re-exposure. Single-cell RNA sequencing at the 24 month timepoint shows similar immune cell proportions and reconstitution of naïve T and B cell subsets in LC and MC. No significant differences in exhaustion scores or antigen-specific T cell clones are observed. These findings suggest resolution of immune activation in LC and return to comparable immune responses between LC and MC over time. Improvement in self-reported health-related quality of life at 24 months was also evident in the majority of LC (62%). PTX3, CRP levels and platelet count are associated with improvements in health-related quality of life.
Climate damage projections beyond annual temperature
Authors: Waidelich Paul , Batibeniz Fulden , Rising James , Kikstra Jarmo S. , Seneviratne Sonia I. 
Published: April 2024

AbstractEstimates of global economic damage from climate change assess the effect of annual temperature changes. However, the roles of precipitation, temperature variability and extreme events are not yet known. Here, by combining projections of climate models with empirical dose–response functions translating shifts in temperature means and variability, rainfall patterns and extreme precipitation into economic damage, we show that at +3 °C global average losses reach 10% of gross domestic product, with worst effects (up to 17%) in poorer, low-latitude countries. Relative to annual temperature damage, the additional impacts of projecting variability and extremes are smaller and dominated by interannual variability, especially at lower latitudes. However, accounting for variability and extremes when estimating the temperature dose–response function raises global economic losses by nearly two percentage points and exacerbates economic tail risks. These results call for region-specific risk assessments and the integration of other climate variables for a better understanding of climate change impacts.
Diversity-dependent speciation and extinction in hominins
Authors: van Holstein Laura A. , Foley Robert A. 
Published: April 2024

AbstractThe search for drivers of hominin speciation and extinction has tended to focus on the impact of climate change. Far less attention has been paid to the role of interspecific competition. However, research across vertebrates more broadly has shown that both processes are often correlated with species diversity, suggesting an important role for interspecific competition. Here we ask whether hominin speciation and extinction conform to the expected patterns of negative and positive diversity dependence, respectively. We estimate speciation and extinction rates from fossil occurrence data with preservation variability priors in a validated Bayesian framework and test whether these rates are correlated with species diversity. We supplement these analyses with calculations of speciation rate across a phylogeny, again testing whether these are correlated with diversity. Our results are consistent with clade-wide diversity limits that governed speciation in hominins overall but that were not quite reached by the Australopithecus and Paranthropus subclade before its extinction. Extinction was not correlated with species diversity within the Australopithecus and Paranthropus subclade or within hominins overall; this is concordant with climate playing a greater part in hominin extinction than speciation. By contrast, Homo is characterized by positively diversity-dependent speciation and negatively diversity-dependent extinction—both exceedingly rare patterns across all forms of life. The genus Homo expands the set of reported associations between diversity and macroevolution in vertebrates, underscoring that the relationship between diversity and macroevolution is complex. These results indicate an important, previously underappreciated and comparatively unusual role of biotic interactions in Homo macroevolution, and speciation in particular. The unusual and unexpected patterns of diversity dependence in Homo speciation and extinction may be a consequence of repeated Homo range expansions driven by interspecific competition and made possible by recurrent innovations in ecological strategies. Exploring how hominin macroevolution fits into the general vertebrate macroevolutionary landscape has the potential to offer new perspectives on longstanding questions in vertebrate evolution and shed new light on evolutionary processes within our own lineage.
Prevalence of breast cancer in rural population of Jaipur: a survey-based observational study
Authors: Singh Roshni , Kumar Sachin , Nakash Prashant , Kumar Ramesh , Kumar Govind , Pal Pusparghya , Mishra Shivang , Raj Preeti , Rajotiya Sumit , Singh Anurag Kumar , Debnath Sourav , Chaturvedi Bhumi , Bareth Hemant , Patel Akhilesh , Singh Mahaveer , Srivastava Anurag , Nathiya Deepak , Tomar Balvir Singh 
Published: April 2024

AbstractBreast cancer, a global health concern predominantly affecting women, recorded 2.3 million new cases and 685,000 deaths in 2020. Alarmingly, projections suggest that by 2040, there could be over 3 million new cases and 1 million deaths. To assess breast cancer prevalence in 24 rural villages within a 60 km radius of NIMS Hospital, Tala Mod, Jaipur, Rajasthan, North India 303,121. A study involving 2023 participants conducted initial screenings, and positive cases underwent further tests, including ultrasound, mammography, and biopsy. SPSSv28 analysed collected data. Among 2023 subjects, 3 screened positive for breast lumps. Subsequent clinical examination and biopsy identified 1 normal case and 2 with breast cancer, resulting in a prevalence proportion of 0.0009 or 98 per 100,000. This study helps fill gap in breast cancer prevalence data for rural Rajasthan. The results highlight a concerning prevalence of breast cancer in the rural area near NIMS hospital, emphasizing the urgent need for increased awareness, early detection, and better healthcare access. Challenges like limited resources, awareness programs, and delayed diagnosis contribute to this high incidence. To address this, comprehensive approach is necessary, including improved screening programs and healthcare facilities in rural areas. Prioritizing rural healthcare and evidence-based strategies can reduce the burden of breast cancer and improve health outcomes.
Alkyl ammonium hydrogen sulfate immobilized on Fe3O4@SiO2 nanoparticles: a highly efficient catalyst for the multi-component preparation of novel tetrazolo[1,5-a]pyrimidine-6-carboxamide derivatives
Authors: Khalaj Mehdi , Musavi Seyed Mahmoud , Ghashang Majid 
Published: April 2024

AbstractIn this, a three-component reaction for the preparation of novel tetrazolo[1,5-a]pyrimidine-6-carboxamide derivatives from N,N′-(sulfonylbis(1,4-phenylene))bis(3-oxobutanamide), aldehydes and 1H-tetrazol-5-amine is reported. The application of Fe3O4@SiO2-(PP)(HSO4)2 (A) as a catalyst afforded the desired products (a1–a18) in high yields in DMF as solvent as well as under solvent-free conditions.
Quantification of play behaviour in calves using automated ultra-wideband location data and its association with age, weaning and health status
Authors: Vázquez-Diosdado J. A. , Doidge C. , Bushby E. V. , Occhiuto F. , Kaler J. 
Published: April 2024

AbstractPlay behaviour can act as an indicator of positive animal welfare. Previous attempts to predict play behaviour in farmed calves are limited because of the classification methods used, which lead to overestimation, and the short time periods that calves are observed. The study aimed to automatically classify and quantify play behaviour in farmed calves using location data from ultra-wide band sensors and to investigate factors associated with play behaviour. Location data were collected from 46 calves in three cohorts for a period of 18 weeks. Behavioural observations from video footage were merged with location data to obtain a total of 101.36 h of labelled data. An AdaBoost ensemble learning algorithm was implemented to classify play behaviour. To account for overestimation, generally seen in low-prevalence behaviours, an adjusted count technique was applied to the outputs of the classifier. Two generalized linear mixed models were fitted to investigate factors (e.g. age, health) associated with duration of play and number of play instances per day. Our algorithm identified play behaviour with > 94% accuracy when evaluated on the test set with no animals used for training, and 16% overestimation, which was computed based on the predicted number of samples of play versus the number of samples labelled as play on the test set. The instances and duration of play behaviour per day significantly decreased with age and sickness, whilst play behaviour significantly increased during and after weaning. The instances of play also significantly decreased as mean temperature increased. We suggest that the quantification method that we used could be used to detect and monitor other low prevalence behaviours (e.g. social grooming) from location data, including indicators of positive welfare.
Recovery of neurophysiological measures in post-COVID fatigue: a 12-month longitudinal follow-up study
Authors: Maffitt Natalie J. , Germann Maria , Baker Anne M. E. , Baker Mark R. , Baker Stuart N. , Soteropoulos Demetris S. 
Published: April 2024

AbstractOne of the major consequences of the COVID-19 pandemic has been the significant incidence of persistent fatigue following resolution of an acute infection (i.e. post-COVID fatigue). We have shown previously that, in comparison to healthy controls, those suffering from post-COVID fatigue exhibit changes in muscle physiology, cortical circuitry, and autonomic function. Whether these changes preceded infection, potentially predisposing people to developing post-COVID fatigue, or whether the changes were a consequence of infection was unclear. Here we present results of a 12-month longitudinal study of 18 participants from the same cohort of post-COVID fatigue sufferers to investigate these correlates of fatigue over time. We report improvements in self-perception of the impact of fatigue via questionnaires, as well as significant improvements in objective measures of peripheral muscle fatigue and autonomic function, bringing them closer to healthy controls. Additionally, we found reductions in muscle twitch tension rise times, becoming faster than controls, suggesting that the improvement in muscle fatigability might be due to a process of adaptation rather than simply a return to baseline function.
Phase-change behavior of RuSbTe thin film for photonic applications with amplitude-only modulation
Authors: Hatayama Shogo , Makino Kotaro , Saito Yuta 
Published: April 2024

AbstractGe2Sb2Te5 (GST), the most mature phase-change materials (PCM), functions as a recoding layer in nonvolatile memory and optical discs by contrasting the physical properties upon phase transition between amorphous and crystalline phases. However, GST faces challenges such as a large extinction coefficient (k) and low thermal stability of the amorphous phase. In this study, we introduce RuSbTe as a new PCM to address the GST concerns. Notably, the crystallization temperature of the amorphous RuSbTe is approximately 350 °C, significantly higher than GST. A one-order-of-magnitude increase in the resistivity contrast was observed upon phase transition. The crystalline (0.35–0.50 eV) and amorphous (0.26–0.37 eV) phases exhibit relatively small band gap values, resulting in substantial k. Although RuSbTe demonstrates a k difference of approximately 1 upon crystallization at the telecommunications C-band, the refractive index (n) difference is negligible. Unlike GST, which induces both phase retardation and amplitude modulation in its optical switch device, RuSbTe exhibits amplitude-only modulation. This study suggests that RuSbTe has the potential to enable new photonic computing devices that can independently control the phase and amplitude. Combining RuSbTe with phase-only modulators could open avenues for advanced applications.