bluuebunny/crossref_metadata_2025_split huggingface dataset:
Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes
Authors: Chades Théo , Le Fèvre Raphaël , Chebbi Imène , Blondeau Karine , Guyot François , Alphandéry Edouard 
Published: February 2024

AbstractWe report the successful fabrication of a pharmaceutical cellular bank (PCB) containing magnetotactic bacteria (MTB), which belong to the Magnetospirillum gryphiswaldense MSR1 species. To produce such PCB, we amplified MTB in a minimal growth medium essentially devoid of other heavy metals than iron and of CMR (Carcinogenic, mutagenic and reprotoxic) products. The PCB enabled to acclimate MTB to such minimal growth conditions and then to produce highly pure magnetosomes composed of more than 99.9% of iron. The qualification of the bank as a PCB relies first on a preserved identity of the MTB compared with the original strain, second on genetic bacterial stability observed over 100 generations or under cryo-preservation for 16 months, third on a high level of purity highlighted by an absence of contaminating microorganisms in the PCB. Furthermore, the PCB was prepared under high-cell load conditions (9.108 cells/mL), allowing large-scale bacterial amplification and magnetosome production. In the future, the PCB could therefore be considered for commercial as well as research orientated applications in nanomedicine. We describe for the first-time conditions for setting-up an effective pharmaceutical cellular bank preserving over time the ability of certain specific cells, i.e. Magnetospirillum gryphiswaldense MSR1 MTB, to produce nano-minerals, i.e. magnetosomes, within a pharmaceutical setting.
Dietary intake and gastrointestinal symptoms are altered in children with Autism Spectrum Disorder: the relative contribution of autism-linked traits
Authors: Li Hailin , Huang Saijun , Jing Jin , Yu Hong , Gu Tingfeng , Ou Xiaoxuan , Pan Shuolin , Zhu Yanna , Su Xi 
Published: February 2024

Abstract
Background
Dietary and gastrointestinal (GI) problems have been frequently reported in autism spectrum disorder (ASD). However, the relative contributions of autism-linked traits to dietary and GI problems in children with ASD are poorly understood. This study firstly compared the dietary intake and GI symptoms between children with ASD and typically developing children (TDC), and then quantified the relative contributions of autism-linked traits to dietary intake, and relative contributions of autism-linked traits and dietary intake to GI symptoms within the ASD group.

Methods
A sample of 121 children with ASD and 121 age-matched TDC were eligible for this study. The dietary intake indicators included food groups intakes, food variety, and diet quality. The autism-linked traits included ASD symptom severity, restricted repetitive behaviors (RRBs), sensory profiles, mealtime behaviors, and their subtypes. Linear mixed-effects models and mixed-effects logistic regression models were used to estimate the relative contributions.

Results
Children with ASD had poorer diets with fewer vegetables/fruits, less variety of food, a higher degree of inadequate/unbalanced dietary intake, and more severe constipation/total GI symptoms than age-matched TDC. Within the ASD group, compulsive behavior (a subtype of RRBs) and taste/smell sensitivity were the only traits associated with lower vegetables and fruit consumption, respectively. Self-injurious behavior (a subtype of RRBs) was the only contributing trait to less variety of food. Limited variety (a subtype of mealtime behavior problems) and ASD symptom severity were the primary and secondary contributors to inadequate dietary intake, respectively. ASD symptom severity and limited variety were the primary and secondary contributors to unbalanced dietary intake, respectively. Notably, unbalanced dietary intake was a significant independent factor associated with constipation/total GI symptoms, and autism-linked traits manifested no contributions.

Conclusions
ASD symptom severity and unbalanced diets were the most important contributors to unbalanced dietary intake and GI symptoms, respectively. Our findings highlight that ASD symptom severity and unbalanced diets could provide the largest benefits for the dietary and GI problems of ASD if they were targeted for early detection and optimal treatment.
A concept for human use of real-time and remote monitoring of diabetic subjects using intermittent scanned continuous glucose measurement
Authors: Goez-Mora Jhon E. , Arbeláez-Córdoba Natalia , Balcazar-Morales Norman , Rivadeneira Pablo S. 
Published: February 2024

Abstract
Background
Flash glucose monitoring systems like the FreeStyle Libre (FSL) sensor have gained popularity for monitoring glucose levels in people with diabetes mellitus. This sensor can be paired with an off-label converted real-time continuous glucose monitor (c-rtCGM) plus an ad hoc computer/smartphone interface for remote real-time monitoring of diabetic subjects, allowing for trend analysis and alarm generation.

Objectives
This work evaluates the accuracy and agreement between the FSL sensor and the developed c-rtCGM system. As real-time monitoring is the main feature, the system's connectivity was assessed at 5-min intervals during the trials.

Methods
One week of glucose data were collected from 16 type 1 diabetic rats using the FSL sensor and the c-rtCGM. Baseline blood samples were taken the first day before inducing type 1 diabetes with streptozotocin. Once confirmed diabetic rats, FSL and c-rtCGM, were implanted, and to improve data matching between the two monitoring devices, the c-rtCGM was calibrated to the FSL glucometer readings. A factorial design 2 × 3^3 and a second-order regression was used to find the base values of the linear model transformation of the raw data obtained from the sensor. Accuracy, agreement, and connectivity were assessed by median absolute relative difference (Median ARD), range averaging times, Parkes consensus error grid analysis (EGA), and Bland–Altman analysis with a non-parametric approach.

Results
Compared to the FSL sensor, the c-rtCGM had an overall Median ARD of 6.58%, with 93.06% of results in zone A when calibration was not carried out. When calibration frequency changed from every 50 h to 1 h, the overall Median ARD improved from 6.68% to 2.41%, respectively. The connectivity evaluation showed that 95% of data was successfully received every 5 min by the computer interface.

Conclusions and clinical importance
The results demonstrate the feasibility and reliability of real-time and remote subjects with diabetes monitoring using the developed c-rtCGM system. Performing calibrations relative to the FSL readings increases the accuracy of the data displayed at the interface.
Smartphone-based inertial measurements during Chester step test as a predictor of length of hospital stay in abdominopelvic cancer postoperative period: a prospective cohort study
Authors: Nascimento Ananda Quaresma , Nagata Letícia Akemi Rosa , Almeida Manuela Trindade , da Silva Costa Vanessa Ladyanne , de Marin Ana Beatriz Rocha , Tavares Victória Brioso , Ishak Geraldo , Callegari Bianca , Santos Enzo Gabriel Rocha , da Silva Souza Givago , de Melo Neto João Simão 
Published: February 2024

Abstract
Background
Objective assessment of pre-operative functional capacity in cancer patients using the smartphone gyroscope during the Chester step (CST) test may allow greater sensitivity of test results. This study has investigated whether the CST is a postoperative hospital permanence predictor in cancer patients undergoing abdominopelvic surgery through work, VO2MAX and gyroscopic movement analysis.

Methods
Prospective, quantitative, descriptive and inferential observational cohort study. Fifty-one patients were evaluated using CST in conjunction with a smartphone gyroscope. Multivariate linear regression analysis was used to examine the predictive value of the CST.

Results
The duration of hospital permanence 30 days after surgery was longer when patients who performed stage 1 showed lower RMS amplitude and higher peak power. The work increased as the test progressed in stage 3. High VO2MAX seemed to be a predictor of hospital permanence in those who completed levels 3 and 4 of the test.

Conclusion
The use of the gyroscope was more accurate in detecting mobility changes, which predicted a less favorable result for those who met at level 1 of the CST. VO2MAX was a predictor of prolonged hospitalization from level 3 of the test. The work was less accurate to determine the patient's true functional capacity.
Frequency and indication of non-musculoskeletal examinations: a cross-sectional survey of Quebec chiropractors
Authors: Giroux Danikel , Branconnier Chloé , Bussières André , Théroux Jean , Blanchette Marc-André 
Published: February 2024

Abstract
Background
Approximately 1% of low back pain is estimated to be caused by serious systemic diseases, including cancer, infection, or abdominal aortic dissection. This study aimed to determine the frequency of execution of non-MSK physical examination procedures among Quebec chiropractors and to identify the clinical context that prompts them to use these physical examination procedures.

Methods
Cross-sectional survey containing 44 questions administered to a random sample of Quebec chiropractors using a succession of online, postal and phone questionnaires. The 4-part survey questionnaire contained six demographic questions, 28 single-choice questions to determine the frequency of execution of non-MSK physical examination procedures, seven short clinical vignettes for which the respondents had to select the non-MSK examinations that would be required, and two questions inquiring about the proportion of new patients for which participants’ felt non-MSK examinations were necessary and whether appropriate assessments were performed. The questionnaire was pilot tested, and feedback received integrated prior to administration. We conducted descriptive statistics, Pearson correlations, and an ANOVA.

Results
The survey was completed by 182 chiropractors (response rate: 36.4%). The most commonly non-musculoskeletal examination performed daily were blood pressure (12.1%) and cranial nerves (4.9%). The most common tests never performed were oxygen saturation (68.7%), cardiac auscultation (69.2%), tibio-brachial index (71.4%), breast (86.8%), rectal (96.7%), testicular (95.6%), and vaginal (99.9%) exams. Female chiropractors and Quebec University in Trois-Rivières graduates reported that a significantly higher proportion of their new patients required a non-musculoskeletal physical examination compared to male participants (37.2% vs 28.3%) or Canadian Memorial Chiropractic College graduates (33.9% vs 19.9%). Reason for not performing a physical examination included the belief that another healthcare professional was better positioned to perform and/or interpret the related tests (76.4%).

Conclusions
Vital signs and cranial nerve examinations were the most frequency performed non-musculoskeletal examinations reported by chiropractors. Apart from the genitourinary exam almost never performed, most participants chose non-musculoskeletal examinations deemed appropriate for the patient’s presentation.
Unlocking hepatocellular carcinoma aggression: STAMBPL1-mediated TRAF2 deubiquitination activates WNT/PI3K/NF-kb signaling pathway
Authors: Wang Zhihuai , Zhang Yinjie , Shen Yuhang , Zhou Haiyang , Gao Yuan , Zhu Chunfu , Qin Xihu 
Published: February 2024

AbstractSTAM Binding Protein Like 1 (STAMBPL1), functions as a deubiquitinase (DUB) and plays a significant role in various types of cancers. However, its effect as a DUB participating in the HCC tumorigenesis and progression still unknown. In the study, the upregulation and strong prognosis value of STAMBPL1 were identified in HCC patients. Functionally, STAMBPL1 significantly promoted HCC cells proliferation and metastasis, and it interacts with TRAF2 and stabilize it via the deubiquitination at the K63 residue. The TRAF2 upregulation stabilized by STAMBPL1 overexpression transfers of P65 protein into the nucleus and activates the WNT/PI3K/ NF-kb signaling pathway. The 251–436 sites of STAMBPL1 particularly interact with the 294–496 sites of TRAF2, thereby exerting the function of DUB and removing the ubiquitin molecules attached to TRAF2. Our research unveiled a new function of STAMBPL1 in mediating TRAF2 deubiquitination and stabilization, thereby activating the WNT/PI3K/NF-kb signaling pathway, suggesting its potential as a novel biomarker and therapeutic target for HCC.
Lessons from an elderly patient with pulmonary embolism caused by protein S deficiency: a case report
Authors: Qiang Liu , Hong Li , Min Shen , Hongping Wang , Xian Chen , Tianlang Li 
Published: February 2024

Abstract
Background
Lower limb deep vein thrombosis (DVT) concurrent with pulmonary embolism (PE) is perilous, particularly in the elderly, exhibiting heterogeneity with thrombophilia mutations. Tailored treatment is essential, yet sudden deaths complicate causative factor elucidation. This report emphasizes genetic testing necessity in PE patients with thrombophilia indicators, facilitating cause identification, personalized treatment guidance, and family education.

Case presentation
This study details a 75-year-old Chinese woman with DVT and PE, where genetic testing identified thrombophilia, guiding personalized treatment decisions.

Results
Upon admission, the patient, after over 10 days of bed rest, presented chest tightness, shortness of breath, and unilateral leg swelling. Diagnostic measures revealed DVT and a substantial PE. Genetic testing identified a PROS1 gene C200A>C mutation, reducing protein S activity. Following 2 weeks of anticoagulation and inferior vena cava filter insertion, the patient, discharged, initiated lifelong anticoagulant therapy. A 1-year follow-up showed no recurrent thrombotic events. Family members carrying the mutation received informed and educational interventions.

Conclusion
Genetic testing for thrombophilic predisposition post-PE is crucial, elucidating etiology, guiding individualized treatment, and playing a pivotal role in family education.
Bernhard Peyer and his discoveries of Triassic vertebrates in Switzerland
Authors: Sues Hans-Dieter 
Published: February 2024

AbstractBernhard Peyer (1885–1963) was a prominent Swiss vertebrate palaeontologist and anatomist at the University of Zurich. In 1919, he discovered the occurrence of Middle Triassic (Anisian–Ladinian) ichthyosaurs, sauropterygians, and other reptiles at Monte San Giorgio on the border between Switzerland and Italy. Starting in 1924, Peyer, and later his student and successor Emil Kuhn-Schnyder, worked tirelessly to excavate and collect material from fossil-bearing deposits in this region. The discovery of an articulated skeleton of a long-necked reptile in 1929 allowed Peyer to solve the riddle of unusually elongate bones from the German Muschelkalk, which, he demonstrated, were cervical vertebrae of the extraordinarily long-necked reptile Tanystropheus. As a student in 1919 and later in 1942, Peyer explored a Rhaetian bonebed close to his native town of Schaffhausen. Processing the sediment, he recovered numerous small bones and teeth of vertebrates, among which teeth of diverse stem-mammals are of particular importance.
Tools to implement measurement-based care (MBC) in the treatment of opioid use disorder (OUD): toward a consensus
Authors: Rush A. John , Gore-Langton Robert E. , Bart Gavin , Bradley Katharine A. , Campbell Cynthia I. , McKay James , Oslin David W. , Saxon Andrew J. , Winhusen T. John , Wu Li-Tzy , Moran Landhing M. , Tai Betty 
Published: February 2024

Abstract
Background
The prevalence and associated overdose death rates from opioid use disorder (OUD) have dramatically increased in the last decade. Despite more available treatments than 20 years ago, treatment access and high discontinuation rates are challenges, as are personalized medication dosing and making timely treatment changes when treatments fail. In other fields such as depression, brief measures to address these tasks combined with an action plan—so-called measurement-based care (MBC)—have been associated with better outcomes. This workgroup aimed to determine whether brief measures can be identified for using MBC for optimizing dosing or informing treatment decisions in OUD.

Methods
The National Institute on Drug Abuse Center for the Clinical Trials Network (NIDA CCTN) in 2022 convened a small workgroup to develop consensus about clinically usable measures to improve the quality of treatment delivery with MBC methods for OUD. Two clinical tasks were addressed: (1) to identify the optimal dose of medications for OUD for each patient and (2) to estimate the effectiveness of a treatment for a particular patient once implemented, in a more granular fashion than the binary categories of early or sustained remission or no remission found in The Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5).

Discussion
Five parameters were recommended to personalize medication dose adjustment: withdrawal symptoms, opioid use, magnitude (severity and duration) of the subjective effects when opioids are used, craving, and side effects. A brief rating of each OUD-specific parameter to adjust dosing and a global assessment or verbal question for side-effects was viewed as sufficient. Whether these ratings produce better outcomes (e.g., treatment engagement and retention) in practice deserves study.
There was consensus that core signs and symptoms of OUD based on some of the 5 DSM-5 domains (e.g., craving, withdrawal) should be the basis for assessing treatment outcome. No existing brief measure was found to meet all the consensus recommendations. Next steps would be to select, adapt or develop de novo items/brief scales to inform clinical decision-making about dose and treatment effectiveness. Psychometric testing, assessment of acceptability and whether the use of such scales produces better symptom control, quality of life (QoL), daily function or better prognosis as compared to treatment as usual deserves investigation.
The displacement mechanism of the cracked rock – a seismic design and prediction study using XFEM and ANNs
Authors: Mughieda Omer , Guo Lijie , Tang Yunchao , Okasha Nader M. , Azimi Sayed Javid , Namdar Abdoullah , Azhar Falak 
Published: February 2024

AbstractMaterials with sufficient strength and stiffness can transfer nonlinear design loads without damage. The present study compares crack propagation speed and shape in rock-like material and sandstone when subjected to seismic acceleration. The nonlinear extended finite element method (NXFEM) has been used in numerical simulation. It assumes the model has a pre-existing crack at 0° from the horizontal. The mechanical properties of the model, crack propagation shape, and crack speed were selected as the main parameters. The nonlinear stress and strain along the crack have been compared in two simulated models. NXFEM and Artificial Neural Networks (ANNs) were used to predict the displacement. The simulation results illustrate that the materials’ crack propagation mechanism and mechanical properties control the stress, strain, and displacement at the selected points in the model. In addition, crack propagation in materials is related to elastic-plastic stresses and strains along the crack path. The speed and shape of the crack are associated with the mechanical properties of the materials. The prediction of crack paths helps to understand failure patterns. Comparison of the seismic response of the rock-like material with sandstone helps to assess the stress, strain, and displacement levels during cracking. This study’s findings agree with the literature report and field observations.
Exploring the significance of caspase-cleaved tau in tauopathies and as a complementary pathology to phospho-tau in Alzheimer’s disease: implications for biomarker development and therapeutic targeting
Authors: Rizzi Liara , Grinberg Lea T. 
Published: February 2024

AbstractTauopathies are neurodegenerative diseases that typically require postmortem examination for a definitive diagnosis. Detecting neurotoxic tau fragments in cerebrospinal fluid (CSF) and serum provides an opportunity for in vivo diagnosis and disease monitoring. Current assays primarily focus on total tau or phospho-tau, overlooking other post-translational modifications (PTMs). Caspase-cleaved tau is a significant component of AD neuropathological lesions, and experimental studies confirm the high neurotoxicity of these tau species. Recent evidence indicates that certain caspase-cleaved tau species, such as D13 and D402, are abundant in AD brain neurons and only show a modest degree of co-occurrence with phospho-tau, meaning caspase-truncated tau pathology is partially distinct and complementary to phospho-tau pathology. Furthermore, these caspase-cleaved tau species are nearly absent in 4-repeat tauopathies. In this review, we will discuss the significance of caspase-cleaved tau in the development of tauopathies, specifically emphasizing its role in AD. In addition, we will explore the potential of caspase-cleaved tau as a biomarker and the advantages for drug development targeting caspase-6. Developing specific and sensitive assays for caspase-cleaved tau in biofluids holds promise for improving the diagnosis and monitoring of tauopathies, providing valuable insights into disease progression and treatment efficacy.
Advanced systems and technologies for the enhancement of user experience in cultural spaces: an overview
Authors: Capece Sonia , Chivăran Camelia , Giugliano Giovanna , Laudante Elena , Nappi Maria Laura , Buono Mario 
Published: February 2024

Abstract
Human–computer interaction, Artificial Intelligence, and the multilingualism of digital culture open up unexpected scenarios in the contemporary design of cultural spaces with the creation of artifacts in which the analogic and digital dimensions come together to enhance the experience. Sensors and devices track user movement in the real world and translate the inputs into commands through hand gestures, speech recognition, head movements, tangible interfaces, or a combination of these elements. Through theorical models, concepts and tools, the paper reports the evolution of “User Experience” applied to personalized enjoyment and use of cultural places. The functional-performance survey of tools and technologies for perception, narrative and augmented interaction revealed models that highlight the diversity and richness of tangible and intangible cultural heritage through new forms of interaction and knowledge transfer. In this direction, new technological tools make it possible to detect, track and evaluate the personalized user experience by processing or producing large amounts of data. Therefore, on one hand the paper explores the limitations dictated by data management and user privacy in using such systems and, on the other, it prefigures new scenarios for amplifying and personalizing the user experience.