bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface Properties of Microcrystalline Hydrous Titanium Oxide as Obtained by Thermal Hydrolysis from a TiCl4 Solution
Authors: Ooi Kenta , Miyai Yoshitaka , Katoh Shunsaku , Sugasaka Kazuhiko 
Published: July 2006

Abstract
The surface properties of hydrous titanium oxides (HTiO’s), as obtained by refluxing TiCl4 or ureacontaining TiCl4 solutions at 373 K, were examined by means of nitrogen adsorption/desorption at 77 K and of fluoride ion adsorption. Their crystal phases and particle shapes were investigated by X-ray diffraction analyses and electronmicroscopic observations, respectively. The HTiO obtained from a 0.03 or 0.1 mol·dm−3 TiCl4 solution was of an anatase type, consisting of small (about 10 nm) particles. They had pores that were 2 nm in radius, with an narrow size distribution. The HTiO obtained from a 1.0 mol·dm−3 TiCl4 solution was of a rutile type, consisting of acicular particles. It had pores that were greater than 2 nm in radius, with a broad size distribution. All of the HTiO’s obtained from (TiCl4+urea) solutions were anatase types. The most probable pore radius increased from 1.9 to 3.6 nm with the increase in the initial TiCl4 concentration from 0.03 to 1.0 mol·dm−3. The surface properties of the anatase-type HTiO’s were discussed based on the sizes and shapes of the primary crystallites and on their spatial distribution.
Binding Aspects of 2-(4-Hydroxyphenylazo)benzoic Acid to Bovine Serum Albumin in the Limited Mixing Ratio
Authors: Takeda Kunio , Sasa Katsushi , Hachiya Kazuaki , Murata Mamoru , Aoki Koichiro 
Published: July 2006

Abstract
The binding of 2-(4-hydroxyphenylazo)benzoic acid (HABA) to bovine serum albumin (BSA) was studied in a dilute phosphate buffer of pH 7.0 and ionic strength 0.014 at 25 °C. Binding measurements were made for both undefatted BSA and defatted BSA (the protein naturally contains 2.4–2.5 mol/mol fatty acid). A metachromasy band appeared at 480 nm at the expense of the original absorption band at 350 nm in the presence of BSA. An isosbestic point in the absorption spectra was observed in the vicinity of 380 nm only in the limited range of the molar mixing ratios of HABA to BSA (D0⁄P0), where the ratio was 1–10 in the above buffer, regardless of the absence and presence of fatty acids. However, the isosbestic point was not observed by any feasible change of the D0⁄P0 ratio in a buffer containing 0.1 M NaCl. This fact seemed to indicate that the binding nature depended on the ionic strength as well as the D0⁄P0 ratio. Then, binding measurements were carried out in the limited range of D0⁄P0 ratios in a dilute phosphate buffer. The amount of HABA bound to BSA was determined by curve-fitting for changes of the apparent molar extinction coefficient, ε, between 300 and 550 nm, assuming one class of binding site: that is, only two species (free and bound dyes) contributing to the absorption changes. The simulated curves of ε were in good agreement with those obtained experimentally. By the use of the Scatchard plot, the binding parameters were determined. The binding constant was larger for defatted BSA than for undefatted BSA, the values being of the order of 105 M−1. The number of binding sites of HABA was 1.4–1.5, not being affected very much by the absence and presence of fatty acids.
Relaxation of Short-Lived 57Fe-Species in 57Co-Labeled Co(IO3)2 Studied by Time-Resolved Emission Mössbauer Spectroscopy
Authors: Watanabe Yasuo , Endo Kazutoyo , Sano Hirotoshi 
Published: July 2006

Abstract
KX-Ray-gated and time resolved emission Mössbauer spectra of 57Co-labeled Co(IO3)2 were measured at room temperature, using a coincidence technique. No remarkable difference was found on the relative intensity of Fe(II)/Fe(III) between the X-ray-gated and normal emission spectra. A lifetime for unstable 57Fe(II)-species formed through EC-decay was estimated to be 43±5 ns and the initial distribution of Fe(II)/Fe(III) at 14.4 keV nuclear level to be 0.47±0.13 from the time-resolved emission Mössbauer spectrum. The results are discussed in terms of the chemical stability of the environments of the decayed 57Fe-atoms.
The Effect of Water Vapor on the Thermal Decomposition of Various Tin(IV) cyclo-Phosphates
Authors: Nariai Hiroyuki , Motooka Itaru , Tsuhako Mitsutomo 
Published: July 2006

Abstract
The process of the thermal decomposition of various tin(IV) cyclo-phosphates, SnP4O12·4H2O(P4m), Sn3(P6O18)2·12H2O (P6m), and Sn2P8O24·5H2O(P8m), was investigated using X-ray analysis, DTA-TG, and HPLC-FIA. P4m lost the water of crystallization at about 105 °C. In the region of 400–500 °C, P4m changed to cyclo-triphosphate (P3m) without any other species of phosphates. Therefore, the change of P4m to P3m probably proceeded as a radical reaction. At temperatures above 580 °C, P3m changed to insoluble polyphosphates (Phigh poly). P6m lost the water of crystallization at about 110 °C. In the region of 500–570 °C, P6m changed to Phigh poly through P3m. P8m lost the water of crystallization at about 140 °C. At about 255 °C, P8m decomposed to oligo phosphates(n&lt;8)(Poligo), soluble polyphosphates(15&lt;n&lt;20)(Ppoly), and amorphous insoluble polyphosphates (Phigh poly*). Under a higher water vapor pressure, these decomposition and subsequent condensation reactions proceeded rather faster than in a lower one. At about 300 °C, Phigh Poiy* changed to P3m again. At higher temperatures, P3m changed to Phigh poiy.
The Crystal Structures of Δ-, Λ-, and Pseudoracemic Forms of Bis(ethylenediamine)oxalatocobalt(III) Aspartates
Authors: Kiriyama Hideko , Igaki Michihito 
Published: July 2006

Abstract
The crystal structures of Δ-[Co(ox)(en)2](l-Hasp)·3H2O, Λ-[Co(ox)(en)2](l-Hasp)·3H2O and rac[Co(ox)(en)2](l-Hasp) have been determined by means of X-ray diffraction. The absolute configurations of (−)-and (+)-cations were assigned as Δ(δλ) and Λ(δλ), respectively, on the basis of that of l-Hasp− ion. The counter ion is extended with C–C–C–C near 180° in all cases, but the other torsion angles are somewhat affected by surroundings. Both α and β carboxyl groups of the anion are ionized, therefore its NH2 group is protonated. In the three compounds, the cations and the anions are alternately arranged, forming a layer through N–H···O hydrogen bonds in a very similar way to each other. The lattice of the more soluble Δ-isomer consists of such single cation–anion layers, between which water molecules are inserted. In the less soluble Λ-diastereoisomer the cation–anion layers are doubled by N–H···O bonds between cations from neighboring layers. The less soluble racemic salt is anhydride where the similar cation–anion layers are connected by hydrogen bonds between cations and between anions alternately. In connection to optical resolution, the difference in solubility was discussed in terms of the density, unoccupied fraction and electrostatic energy.
Studies on the Reaction Products of Antitumor Platinum Complex with d(ApG), d(GpA), and d(pGpA). 2′-Deoxy Sugar Is Favourable for G-N7, A-N1 Chelation
Authors: Inagaki Kenji , Tomita Akihito , Kidani Yoshinori 
Published: July 2006

Abstract
The reaction products of dichloro[(1R,2R)-1,2-cyclohexanediamine]platinum(II), Pt(R,R-dach)Cl2, with dinucleotides, d(ApG), d(GpA), and d(pGpA) have been separated by HPLC, and each product fractionated has been characterized by UV and CD spectral analyses. The reaction of Pt(R,R-dach)Cl2 with d(ApG) yields Pt(R,R-daich)(d(ApG)-N1(1),N7(2)) (1, 16%), Pt(R,R-dach)(d(ApG)-N7(1),N7(2)) (2, 80%) and uncharacterized minor product (4%). The reaction with d(GpA) yielded two Pt(R,R-dach)(d(GpA)-N7(1),N1(2)), (3, 22% and 5, 61%), and Pt(R,R-dach)(d(GpA)-N7(1),N7(2)), (4, 17%), but surprisingly 5 isomerizes very slowly to 3 and 4 under the subsequent incubation (half life of 5; about 6 days at 37 °C). The reverse reaction (3 and 4→5) was not observed. The reaction with d(pGpA) also yielded two Pt(R,R-dach)(d(pGpA)-N7(1),N1(2)), (3′, 8% and 5′, 80%) and Pt(R,R-dach)(d(pGpA)-N7(1),N7(2)), (4′, 12%). The adduct, 5′, also isomerizes to 3′ and 4′. The platinum adducts, 3′, 4′, and 5′, are compatible with 3,4, and 5, respectively, after removal of the 5′-phosphate group upon treatment with alkaline phosphatase. A competition reaction between d(ApG) and d(GpA) for Pt(R,R-dach)Cl2 indicates that d(ApG) was about 2 times more reactive than d(GpA). The reactivity decreases along the series; d(pGpA)&gt;d(ApG)&gt;d(GpA).
Synthesis and Properties of Copper(II) Thiocyanato Complexes with Imino Oximes
Authors: Wan Songping , Mori Wasuke , Yamada Shoichiro , Murahashi Shun-Ichi 
Published: July 2006

Abstract
Reaction of copper(I) thiocyanate with imino oximes 3-[[2-(alkylamino)ethyl]imino]-2-butanone oximes or 3-[[2-(dialkylamino)ethyl]imino]-2-butanone oximes, (abbreviated as Hdox-enRR′), gave a series of copper(II) complexes which consist of binuclear complexes [Cu2(dox-enRR′)2(NCS)2] with a thiocyanate anion coordinated to the copper(II) ion. The magnetic susceptibilities over the temperature range 77–320 K show a strong antiferromagnetic spin coupling through the N–O bridge for these complexes. The magnetic behavior can be explained by using the Bleaney–Bowers equation.
Photochemical Alkylation, Hydroxyalkylation, and Alkoxylation of Pyridinecarboxamides in Alcohol
Authors: Sugimori Akira , Itoh Hiroshi , Kanai Mitsuharu , Itoh Nobuko , Sugiyama Toru 
Published: July 2006

Abstract
The UV-irradiation of 2- and 4-pyridinecarboxamides in alcohol brings about alkylation and/or hydroxyalkylation in the pyridine ring. In the irradiation of 3-pyridinecarboxamide in the presence of sulfuric acid, ionic reaction (alkoxylation at the 2- and 6-position) and radical reaction (alkylation at the 4- and 6-position) occur in parallel. The effects of quenchers indicate that two alkylation products originate from one excited triplet state which is quenched by energy-transfer mechanism and that two alkoxylation products originate from the excited singlet state.
Novel Sulfur-Containing Phytoalexins from the Chinese Cabbage Brassica campestris L. ssp. pekinensis (Cruciferae)
Authors: Takasugi Mitsuo , Monde Kenji , Katsui Nobukatsu , Shirata Akira 
Published: July 2006

Abstract
The first isolation and structure elucidation of four sulfur-containing indole phytoalexins from Chinese cabbage inoculated with Pseudomonas cichorii are described.
Iodine- or Iodine Monobromide-Catalyzed Alkoxy–Alkoxy Exchange Reactions of Alkylalkoxysilanes: Formation of the Catalyst–Alkoxysilane Complexes and the Reaction Mechanism
Authors: Ito Katsuko , Ibaraki Takeshi 
Published: July 2006

Abstract
The formation of charge-transfer complexes of iodine and of iodine monobromide with alcohols and alkoxysilanes has been established spectroscopically, and the formation constants of iodine–ethoxytriethylsilane and iodine–diethoxydimethylsilane complexes has been determined as 0.55±0.01 and 0.61±0.02, respectively. On the basis of these observations and the kinetic information recently reported, the previously proposed mechanism for the iodine or iodine monobromide catalyzed alkoxy–alkoxy exchange reactions of alkoxysilanes is discussed afresh. It has been confirmed that a mechanism involving a four-centered transition state containing a CT-complex is most favorable.
Dual-Mode Behavior in Doping–Undoping of Polypyrrole with Alkanesulfonate
Authors: Shimidzu Takeo , Ohtani Akira , Honda Kenichi 
Published: July 2006

Abstract
Polypyrrole (PPy) films incorporated with a series of 1-alkanesulfonates, RnSO3− (CnH2n+1SO3−, n=3–10), were prepared by the electrochemical polymerization of pyrrole in H2O containing sodium salt of RnSO3−. The mass transfer in the PPy matrix of the RnSO3− incorporated PPy (PPy/RnSO3−) films were investigated by electrochemical measurements. The electrochemical properties of PPy/RnSO3− were affected by the chain length of the incorporated RnSO3−. The incorporation and release of cationic species into the PPy/RnSO3− matrix during an electrochemical oxidation–reduction of PPy were observed and the contribution of cationic species during the doping-undoping process was increased with the dopant size. The PPy/RnSO3− showed a dual-mode behavior during the electrochemical doping–undoping process.
Novel Syntheses of Arylcarbamic Esters from Carbon Dioxide and Aromatic Amine via a Zinc Carbamate
Authors: Yoshida Yasuhiko , Ishii Shigeru , Kawato Akinori , Yamashita Tadataka , Yano Masahiro , Inoue Shohei 
Published: July 2006

Abstract
Arylcarbamic esters were synthesized directly from carbon dioxide and an aromatic amine via a zinc carbamate. The alkylation of the reaction mixture of carbon dioxide, an aromatic amine, and diethylzinc with dialkyl sulfate formed alkyl arylcarbamate in a high yield. Also, 2-hydroxycyclohexyl diphenylcarbamate was obtained selectively in a good yield by the reaction of carbon dioxide, epoxycyclohexane, and ethylzinc diphenylamide. Other arylcarbamates of 1,2-cyclohexanediol were obtained by the reactions of carbon dioxide, an aromatic amine, diethylzinc, and epoxycyclohexane.
Retentive Solvolysis. 15. Salt Effect on the Retentive Phenolyses of 1-(p-Substituted phenyl)ethyl p-Nitrobenzoates. The Pattern of Salt Effect and the Number of Ion-Pair Intermediates in the SN1 Solvolysis
Authors: Kinoshita Tomomi , Shibayama Koichi , Ikai Keizo , Okamoto Kunio 
Published: July 2006

Abstract
The salt effect of sodium phenoxide on the polarimetric (kp) and titrimetric rate constants (kt) has exhibited pattern B for the phenolysis of optically active 1-(p-methylphenyl)ethyl p-nitrobenzoate (ROPNB; (1)) in pure phenol. The other patterns A, C, and D were previously observed for the phenolyses of 1-phenylethyl p-nitrobenzoates with p-MeO- (2), p-H- (3), and p-NO2-substituents (4), respectively. Thus, the kp–kt pattern changes in the order A→B→C→D as the stability of the intermediate decreases in the order of 2&gt;1&gt;3&gt;4. All the kp–kt patterns can be correlated with the ion-pair stage for product formation, i.e., the pattern A with the second ion-pair intermediate and the patterns B, C, and D with the first one. The pattern of salt effect on the product distribution (% of ROPh, o- and p-RC6H4OH, and p-MeC6H4CH=CH2) for 1 is also compatible with the kp–kt pattern B.
Synthesis and Properties of Dinitromethylated Arenes. Reinvestigation of the Ponzio Reaction
Authors: Suzuki Hitomi , Takaoka Hiroshi , Yamamoto Hidetoshi , Ogawa Takuji 
Published: July 2006

Abstract
Several title compounds have been prepared by the modified Ponzio procedure and their IR, 1H and 13C NMR data are presented. In some cases trinitromethylated arenes were obtained as a by-product. Oxidative cleavage to parent aldehydes and oxidation to carboxylic acids were the major competing reactions. 9-Anthracenecarbaldehyde oxime and 10-methyl-9-anthracenecarbaldehyde oxime reacted with nitrogen dioxide in an unexpected way, giving 10-nitro-9-anthracenecarbonitrile oxide and 10-methyl-10-nitro-9-anthrone as the respective main products. Nitration of (dinitromethyl)benzene has also been examined under various conditions.
Effect of Pore Diffusion in Liquid-Phase Enantioselective Hydrogenation with Ni–SiO2 Catalysts
Authors: Nitta Yuriko , Imanaka Toshinobu 
Published: July 2006

Abstract
A series of Ni–SiO2 catalysts, having a constant Ni loading and different Ni crystallite sizes, were prepared by using series of silica supports with controlled wide-pore structure. The crystallite size effect in enantioselective hydrogenation was found to hold also for the reactions controlled by the pore diffusion.
Kinetic Studies of Fast Equilibrium by Means of High-Performance Liquid Chromatography. XVII. Separation of Tautomers of 1,3-Cyclohexanediones
Authors: Moriyasu Masataka , Kato Atsushi , Hashimoto Yohei 
Published: July 2006

Abstract
Tautomers of 1,3-cyclohexanediones have been separated by means of low-temperature high-performance liquid chromatography (HPLC). The ratios of keto and enol-forms have been found to be highly solvent dependent, which is explained in terms of solute–solvent and solute–solute hydrogen bonding.
Photo-Excitable Membranes. Effects of pH of the Solution on the Photoresponse of Poly(vinyl chloride) Membrane Doped with a Spirobenzopyran Derivative
Authors: Anzai Jun-ichi , Hasebe Yasushi , Ueno Akihiko , Osa Tetsuo 
Published: July 2006

Abstract
The magnitude of the photoinduced membrane potential across a poly(vinyl chloride)/spirobenzopyran membrane depends considerably on the pH of the solution adjacent to the membrane surface which is exposed to light. The photoresponse is enhanced in the acidic region, while is depressed in the alkaline region. The results are explained in terms of the formation of the asymmetric membrane and of the charge density on the membrane surface.
Effect of the Aggregation Number on Gel Chromatographic Patterns of Micellar Systems
Authors: Funasaki Noriaki , Hada Sakae , Neya Saburo 
Published: July 2006

Abstract
Gel chromatographic patterns and their derivatives of micellar systems are predicted as a function of the total surfactant concentration and the aggregation number m by plate theory and asymptotic theory. These predicted patterns provide the basis for estimation of monomer concentration and m from experimental chromatographic data.
Preparation of Highly Pure Lithium and Sodium Fluorides Using Solvent Extraction
Authors: Kobayashi Kenji 
Published: July 2006

Abstract
LiF and NaF can be purified by solvent extraction. The impurity concentrations in the purified NaF are 2.5 ng g−1 for chromium, 60 ng g−1 for iron, 0.03 ng g−1 for cobalt, and 1.5 ng g−1 for copper. The concentrations in the purified LiF are 42 ng g−1 for nickel and 2.1 ng g−1 for copper.
Synthesis of Bis[1,2-bis(methylseleno)vinylenedithio]tetrathiafulvalene (TMSVT)
Authors: Nakano Hideyuki , Nogami Takashi , Shirota Yasuhiko 
Published: July 2006

Abstract
The title molecule was synthesized as an effective donor molecule for highly conductive organic complexes. It is a TTF dirivative possessing four selenium atoms and eight sulfur atoms. Its oxidation potential (E1⁄2) is 0.86 V vs. SCE in THF, which is slightly higher than that of VT and TMTVT.
Efficient Synthesis of Sterically Hindered Chiral Binaphthol Derivatives
Authors: Maruoka Keiji , Itoh Takayuki , Araki Yoshitaka , Shirasaka Tadashi , Yamamoto Hisashi 
Published: July 2006

Abstract
A highly effective method for preparation of a variety of sterically hindered chiral binaphthol derivatives has been described which involves the intramolecular 1,3-rearrangement of bis(trialkylsilyl ether) of chiral 3,3′-dibromo-1,1′-bi-2-naphthol with t-BuLi.
Crystal Structures of the Imidazole and the 1,4-Di(1-imidazolyl)butane Adducts of N,N′-Ethylenebis[salicylideneaminato]manganese(III) Perchlorate
Authors: Matsumoto Naohide , Takemoto Naohiko , Ohyosi Akira , Okawa Hisashi 
Published: July 2006

Abstract
Crystal structures of the titled compounds [Mn(Him)(salen)][ClO4]CH3OH (1) and [Mn(biim)(salen)][ClO4] (2), have been determined by the single-crystal X-ray diffraction method. The molecular structure of the imidazole adduct (1) is a binuclear structure bridged by phenoxy oxygen atoms of salen ligand, while that of the 1,4-di(1-imidazolyl)butane adduct (2) is a polynuclear structure bridged by imidazole nitrogen atoms of 1,4-di(1-imidazolyl)butane.
The Determination of a Small Amount of Biological Constituent by the Use of Chemiluminescence. XIV. The Flow-Injection Analysis of Protein Using Ultrasonic Chemiluminescence of Luminol
Authors: Hara Tadashi , Nakatsu Kazuhiro , Arai Akihiro , Yoshida Tatsunari , Tsukagoshi Kazuhiko 
Published: July 2006

Abstract
A flow-injection analysis method for the determination of a small amount of protein has been established by the use of the ultrasonic chemiluminescence of luminol. The present method was based on the phenomenon that the catalytic activity of cobalt(II) for the ultrasonic chemiluminescence of luminol decreased in the presence of protein. The apparatus consisted of only a luminol solution line equipped with an ultrasonic irradiation bath and a cobalt(II) catalyst solution line equipped with a sample inlet. Under optimum conditions, similar calibration curves were obtained for bovine serum albumin, bovine serum γ-globulin, human serum albumin, and human serum γ-globulin. According to the present method, these proteins could be determined in the concentration range 5.0×10−6–1.0×10−1 g dm−3, with a detection limit of 200 pg (injected sample volume 40 mm3, S/N=2) and a coefficient of variation of 2.9% (n=8) at a rate of about 60 samples per hour.
Ligand-Substitution Reaction Kinetics of Tris(8-quinolinolato)- and Bis(2-methyl-8-quinolinolato)(8-quinolinolato)aluminum(III) Complexes with Ethylenediamine-N,N,N′,N′-tetraacetic Acid
Authors: Horihata Tomonori , Okamoto Ken-ichi , Hidaka Jinsai , Einaga Hisahiko 
Published: July 2006

Abstract
Ligand-substitution reactions of [Al(hq)3] and [Al(hq)(mhq)2] (H(m)hq: (2-methyl-)8-quinolinol) with H4edta have been studied at 25 °C and 1.0 mol dm−3 NaCl by means of the stopped-flow spectrophotometric method. Three concurrent reaction pathways, a dissociative activation one and two parallel associative activation ones, to which H2edta2− and Hedta3− contribute, are elucidated.
Chemistry of Germanium Atoms. IV. A New Method for Preparation of Organogermanium Halides by Co-condensation of Germanium Vapor with Tetrahydrofuran
Authors: Mochida Kunio , Nakai Y\={u}ko , Shiota Shigeru 
Published: July 2006

Abstract
Germanium vapor (atom) when co-condensated with THF at 77 K was found to be reactive toward organic halides. Several organogermanium halides were prepared by the addition of organic halides to the resultant germanium atom-THF slurry at ambient temperatures.
Facile Photorearrangement of Some Crowded Azoxyarenes via Two Concurrent Pathways
Authors: Okubo Masao , Hyakutake Hayato , Taniguchi Naoki 
Published: July 2006

Abstract
Naphthalene-1-ONN-azoxybenzenes and some unsymmetrically ortho-substituted ONN- and NNO-azoxybenzenes were prepared, and their photochemical behavior in ethanol was compared. The crowded azoxyarenes tend to undergo a facile Wallach rearrangement via the known azoxy-ortho oxygen mirgation and a concurrent pathway involving ONN–NNO isomerization. The relative contribution of the two pathways was affected by substituents.
Novel Synthesis of Isoflavones by the Palladium-Catalyzed Cross-Coupling Reaction of 3-Bromochromones with Arylboronic Acids or Its Esters
Authors: Hoshino Yukio , Miyaura Norio , Suzuki Akira 
Published: July 2006

Abstract
The synthesis of isoflavone derivatives by means of palladium-catalyzed cross-coupling reaction between 3-bromochromones and arylboronic acids or its butyl esters is described.
Determination of a Small Amount of a Biological Constituent by the Use of Chemiluminescence. XII. Highly Sensitive Immunoaffinity Chromatography
Authors: Hara Tadashi , Tsukagoshi Kazuhiko , Arai Akihiro , Iharada Takeshi 
Published: July 2006

Abstract
Highly sensitive immunoaffinity chromatography was established by combining a micro-bore affinity column with a chemiluminescence detector for protein monitored by a flow-injection method using a 1,10-phenanthroline–hydrogen peroxide–copper(II) system. After having optimized the bed volume of a column, the flow rate of a buffer solution which was used for an immune reaction, and the time necessary for an immune reaction in a column, human serum albumin in the range of 3×10−4–1×10−1 g dm−3 could be determined, with the detection limit of 15 ng as the injected amount. Human serum γ-globulin and bovine serum γ-globulin showed somewhat nonspecific adsorption onto an affinity column with values of 0.16 and 0.14 based on the specific adsorption 1.00 of human serum albumin onto a column, whereas bovine serum albumin, bovine serum α-globulin, and ovalbumin showed no nonspecific adsorption.