bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monoclonal antibodies directed against lipopolysaccharide of legionella pneumophila serogroup 1
Authors: Baranov A. M. , Vetchinin S. S. , Baranova E. V. , Biketov S. F. , Svetoch E. A. 
Published: July 2007

AbstractThe monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG‐secreting. The MAbs had narrow strain specificity mad showed no cross‐reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.
The effect of interferon therapy on erythrocyte membrane Na, K ATP activity in patients with chronic hepatitis B and C virus infections
Authors: Kuralay Filiz , Tanyalçin Tijen , Kutay Fatma , Ersöz Galip , Yüce Gül , Batur Yücel 
Published: July 2007

AbstractIn order to evaluate the effect of alpha interferon on erythrocyte membrane Na+, K+ ATPase (EC 3.6.1.37) activity, 10 patients with chronic hepatitis B virus (HBV) infection and 8 patients with chronic hepatitis C virus (HCV) infection were investigated. Erythrocyte membrane Na+, K+ ATPase activity was determined in controls and in patients with HBV and HCV infection. Na+, K+ ATPase activity was significantly less in untreated patients with (HBV) infection (n=20; 0,134±0,073 μmol of phosphate produced per milligram of protein per hour) and (HCV) infection (n=11; 0,144±0,049) when compared to the controls (n=10; 0,219±0,055). Among these subjects patients were treated with interferon and following treatment, significant elevation of Na+, K ATPase activity was seen in patients with HCV (n=8; 0,183±0,044; P=0,049) and HBV (n=10, 0,213±0,095, P=0,0069) infections when compared with the pre‐treatment values (n=8; 0,152±0,050) and (n=10, 0,131±0,083), respectively. Normalization of serum alanin amino transferase levels (ALT) at treatment cessation was seen in 8 of 10 (%80) HBV infected patients of whom 2 of 8 (%25) had sustained ALT responses within three months after the end of treatment. In HCV infected patients 1 of 8 (%12,5) had sustained response following treatment. At the end of treatment, although Na+, K+ ATPase was restored in both of the patients groups, relative changes in enzyme activity in relation to relative reduction in ALT levels as a response to IFN therapy were not correlated.
A comparative study by a single chromatographic procedure of glycolytic regulatory kinase isozymes in rat erythroid cells as a function of differentiation‐maturation process
Authors: Jimeno Pilar , Luque José , García‐Pérez Ana I. , Pinilla Montserrat 
Published: July 2007

AbstractThe isozymes of three glycolytic regulatory kinases: hexokinase, phosphofructokinase and pyruvate kinase are fractionated by a single ion exchange chromatographic procedure on DEAE‐cellulose. Enriched‐erythroblast bone marrow cells showed two heterogeneous peaks, each consisting of two overlapping peaks: one major and one minor peak, but only two isozymes were observed in reticulocytes and erythrocytes. Phosphofructokinase showed multiple isozymic forms in the three cell populations, but while in erythroblasts the main one eluted in the last fractions, in reticulocytes and erythrocytes it eluted in the early fractions. Pyruvate kinase showed a main early activity peak with a shoulder in erythroblasts, reticulocytes and erythrocytes but the response to the allosteric effectors (fructose‐1,6‐bisphosphate and ATP) suggests the presence of different pyruvate kinase isozymes in reticulocytes and erythrocytes.
5‐Azacytidine modulates γ‐glutamyltransferase and glutathione levels in two human prostatic adenocarcinoma cell lines
Authors: Yaagoubi M. El , Visvikis A. , Khalid A. , Siest G. , Wellman M. 
Published: July 2007

AbstractTwo human prostate adenocarcinoma cell lines, LNCaP and PC‐3, were used to study the effect of 5‐azacytidine on GGT gene expression, via genomic DNA methylation, and GSH content. When the cells were treated with 5‐azaC, the specific GGT activity increased in a dose and time‐dependent manner and was accompanied by the elevation of intracellular glutathione content. Southern blot analysis of DNA digested with MspI or HpaII showed negative correlation between the methylation pattern of GGT DNA and GGT activity, either in control or 5‐azaC treated cells. Reverse transcription‐polymerase chain reaction (RT‐PCR) analysis revealed that GGT mRNA types I are expressed and induced in a cell type‐specific manner in control and 5‐azacC treated cells respectively. Overall, our investigations suggest that DNA methylation and other mechanisms combine to regulate GGT gene expression in studied prostate adenocarcinoma derived cells.
Cooperative and differential effects of estrogen, prolactin, 22K and 20K human growth hormones on cyclin D1/PRAD1 gene expression in T‐47D human breast cancer cells
Authors: Kaneko Hiroshi , Fujikawa Takahiko , Alam Khorshed S. M. , Kaneko Satoshi , Tanaka Minoru , Hibasami Hiroshige , Nakashima Kunio 
Published: July 2007

AbstractThe cyclin D1/PRAD1 gene is correlated with carcinogenesis of human breast cancer. In this study, we have analyzed effects of breast cancer‐related hormones on the cyclin D1 gene expression in T‐47D human breast cancer cells. Estradiol (E2) and human prolactin (hPRL) equally enhanced the cyclin D1 gene expression in the cells, and 22 and 20 kDa human growth hormones (22K and 20K hGHs) showed less stimulatory effects. In the presence of E2, however, hPRL or 22K hGH showed additive stimulations of the cyclin D1 gene expression to that by E2 alone, while 20K hGH did not show any additive stimulation of the gene expression. The results suggest that the signal pathways through estrogen and hPRL receptors are important for cyclin D1 gene expression in breast cancer cells, and that 20K hGH has little effect on the cyclin D1 gene expression in these cells because of its lower affinity to PRL receptor.
Study of the influence of salt concentration on Newcastle disease virus matrix protein aggregation
Authors: Sagrera Ana , Cobaleda César , Berger Sylvia , Marcos María José , Shnyrov Valery , Villar Enrique 
Published: July 2007

AbstractThe aggregation process of Newcastle disease virus matrix protein (M protein) has been studied using light scattering. We observed that the aggregation of M protein is inversely correlated with ionic strength and that this process can be reversed by high salt concentrations. It was found that the oligomeric structure of NDV matrix protein is different from those described earlier for other matrix proteins of enveloped viruses
Antiidiotypic antibody to gonadotropin releasing hormone as probe for cell surface receptors
Authors: Karande Anjali A. , Rajeshwari Krishnamurthy , Padmanaban Deepa , Ravichandra Balijepalli , Joshi Preeti G. , Yadav Poonam , Shobha Prahlad 
Published: July 2007

AbstractMonoclonal antibodies were raised to the idiotype of a gonadotropin releasing hormone (GnRH) specific antibody, one of which was found to bind specifically to GnRH receptors present on pituitary gonadotrophs, placental syncytiotrophoblasts and testicular Leydig cells. These observations were confirmed by Western and ligand blotting as well as by the ability of the antibody to induce an increase in intracellular Ca++ in a mouse gonadotroph cell‐line viz., αT3‐1.
Dietary fats alter the activity and expression of glucose‐6‐phosphate dehydrogenase in rat lymphoid cells and tissues
Authors: Otton Rosemari , Graziola Fabiana , Hirata Mário H. , Curi Rui , Williams John F. 
Published: July 2007

AbstractThe effect of diets enriched with fat containing different fatty acids on the activity and expression of the glucose‐6‐phosphate dehydrogenase (EC 1.1.1.49) of mesenteric lymph nodes, lymphocytes and intraperitoneal macrophages was examined. Measurements of the enzyme were also performed using spleen, thymus and liver for comparison. The following fat rich diets containing a variety of fatty acids were used: 1‐standard chow (CC); 2‐medium chain saturated fatty acids (MS)‐cocconut fat‐oil; 3‐long chain saturated fatty acids (LS)‐cocoa butter; 4‐monounsaturated fatty acids (MU)‐canota oil (n‐9); 5‐polyunsaturated fatty acids (PU)‐soybean oil (n‐6). Of the fat‐rich diets tested, MS had the least effect. The G6PDH activity of lymphocytes was reduced by all the fat‐rich diets; 16% for MS, 38% for LS, and 54% for MU. Similarly, the enzyme activity was reduced in macrophages; 35%, 86%, and 73%, for LS, MU, and PU. respectively. In contrast, the fat‐rich diets elevated G6PDH activity in the lymphoid organs; by 42% in the spleen due to LS and by 131%, 35%, and 56% in the thymus due to LS, MU, and PU, respectively. Fat‐rich diets decreased the activity of G6PDH in liver: 42%, 68%, and 39% for MS, MU, and PU, respectively. Some of the changes in G6PDH activity induced by the fat‐rich diets occur through the mechanisms of mRNA abundance.
DNase I hypersensitive Sites in the chromatin of the chicken Msx2 gene differ in anterior and posterior lim mesenchyme, calvarial osteoblasts and embryonic fibroblasts
Authors: Pan ZhongZong , Lichtler Alexander C. , Upholt William B. 
Published: July 2007

AbstractTo identify candidate cis‐acting regulatory regions involved in regulation of the Msx2 gene in anterior limb mesenchyme and calvarial osteoblasts, DNase I hypersensitive sites (DHSs) from ‐6.1 kb to +8.4 kb relative to the translation start site of the chicken Msx2 gene were identified in anterior and posterior limb mesenchyme, calvarial osteoblasts, and embryonic fibroblasts. A total of 12 DHSs were detected. Except for the DHS in the basal promoter region, none of the other DHSs were present in all four tissues, suggesting that the chromatin structure in the Msx2 gene locus is differently organized in these four cell types. One DHS was unique to Msx2 expressing cells and a second site to nonexpressing cells. Anterior and posterior limb mesenchyme had similar patterns of DHSs that were much more complex than observed in catvarial osteoblasts possibly reflecting differences in the complexity of Msx2 regulation in these two tissues.
Effect of aminoguanidine on lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney
Authors: Kędziora‐Kornatowska K. , Luciak M. 
Published: July 2007

AbstractThe aim of this study was to examine lipid peroxidation and activities of key antioxidant enzymes in kidneys of rats with streptozotocin (STZ)‐induced diabetes and the effect of aminoguanidine on diabetes‐induced alterations. Three groups, 6 rats each, were studied: control animals, not treated diabetic rats and rats treated with aminoguanidine (AG; 1 g/liter of drinking water). After 6 and 12 weeks the animals were sacrificed and lipid peroxidation products and activities of antioxidant enzymes were determined in their kidney homogenates. Malondialdehyde (MDA) content was significantly elevated and activities of SOD and catalase decreased in the kidneys of STZ‐diabetic rats. AG treatment attenuated the increase in MDA content and diminutions of activities of SOD and catalase in the kidneys of diabetic rats. These results confirm oxidative stress in the kidney of rats with STZ diabetes and point to an antioxidant effect of AG in experimental diabetes.
Role of DNA definite structural elements in interaction with repair enzyme uracil‐DNA glycosylase
Authors: Kubareva Elena A. , Vasilenko Natalja L. , Vorobjeva Olga V. , Volkov Evgeny M. , Oretskaya Tatjana S. , Korshunova Galina A. , Nevinsky Georgy A. 
Published: July 2007

AbstractInteraction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil‐DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG‐substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme‐substrate recognition. However the phosphate group adjacent to single dU from the 3′‐end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2′‐deoxy‐2′‐aminouridine is a non‐hydrolyzable substrate analog for UDG.
The use of a chimera HIV‐1/HIV‐2 envelope protein for immunodiagosis of HIV infection: Its expression and purification in E. coli by use of a translation initiation site within HIV‐1 env gene
Authors: Han Baoguang , Song Xiaoguo , Chen Qun , Wang Hongyan , Ling Shigan 
Published: July 2007

AbstractA chimera HIV‐1/HIV‐2 envelope sequence composed of multiple conserved immunodominant epitopes of HIV‐1 envlope protein (HIV‐1 IIIB: env482‐518+env548‐675) and the HIV‐2 gp36 immunodominant epitope (env592‐603), was constructed and directly over‐expressed in E. coli by using a prokaryotic translation initiation sequence contained within the gene of HIV‐1 envelope. The recombinant product was purified and applied in antibody‐screening assay. The purified chimera antigen reacted with all the thirty‐eight HIV‐1 positive serum samples, the two HIV‐2 serum samples, and had no cross‐reaction with all the eighty‐eight normal healthy serum sample. The results indicated that this recombinant chimera HIV‐1/HIV‐2 envelope protein could be useful for diagnostic purposes of HIV infection.
Three dimensional electron spin resonance (ESR) imaging of internal organs in living mice
Authors: Masumizu Toshiki , Fujii Kanae , Kohno Masahiro , Nagai Soichi , Odagaki Yuji , Imanari Mamoru , Mori Akitane , Packer Lester 
Published: July 2007

AbstractIn viva electron spin resonance imaging (ESR imaging) was applied to living mice after peroral administration of a nitroxide radical spin probe. A 3D ESR imaging procedure was applied in vivo in order to obtain the exact distribution of the spin probe in a living animal. The imaging pictures demonstrated that the administered spin probe was firstly located in the stomach, then delivered to the liver, kidney and heart of the animal.
Glucose‐ and phorbol ester‐induced insulin secretion in human insulinoma cells ‐ Association with protein kinase C activation‐
Authors: Miura Atsushi , Ishizuka Tatsuo , Itaya Satomi , Ishizawa Masayoshi , Kanoh Yoshinori , Kimura Mika , Kajita Kazuo , Yasuda Keigo 
Published: July 2007

AbstractThis study examined the effect of glucose and 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) on insulin secretion in isolated human insulinoma cells. In addition, we analyzed conventional PKCα and β activation in the membrane fractions, respectively. Treatment with 5 mM and 20 mM glucose for 5 min and 20 min resulted in 6∼7‐fold increases in insulin secretion, and treatment with 1 μM TPA for 5 min also resulted in 3‐fold increases in insulin secretion from the basal level. Immunoblot analysis of membrane fractions showed increases in PKCα and β immunoreactivities after treatment with 5 mM, 20 mM glucose and 1 μM TPA. Translocations of PKCα after treatment with glucose and TPA were greater than those of PKCβ in membrane fractions. These results suggest that TPA independently provokes insulin secretion via PKC activation and that PKCα and β activation may be involved in insulin secretion in human insulinoma cells.
Occurrence of bovine spleen CD38/NAD glycohydrolase disulfide‐linked dimers
Authors: Berruet Laure , Muller‐Steffner Hélène , Schuber Francis 
Published: July 2007

AbstractBovine spleen NAD+ glycohydrolase, an ecto‐enzyme closely related to CD38, catalyzes the conversion of NAD+ into ADP‐ribose and cyclic ADP‐ribose, a calcium‐mobilizing metabolite. We have raised polyclonal antibodies against the native enzyme which on immunoblots revealed, besides the 32 kDa monomer, the presence of a stable dimeric form. This dimerization was shown to result from a spontaneous oxidative process involving the formation of one or several disulfide bond(s) sensitive to reducing agents such as 2‐mercaptoethanol. The homodimeric oxidized enzyme, which was not detected during the early steps of the enzyme purification procedure, was catalytically active. Our results underline the differences, in terms of oligomerization and reactivity towards thiols, between CD38/NAD+ glycohydrolases depending on their origin.
A Mg2 ‐dependent endonuclease is responsible for internucleosomal DNA fragmentation in human B lymphoblastic IM9 cells
Authors: Kwon Hyung‐Joo , Kim Doo‐Sik 
Published: July 2007

AbstractWe have identified a Mg2+‐dependent endonuclease activity from human B lymphoblastic IM9 cell lysates and nuclei using autodigestion method and DNA‐native‐polyacrylamide gel electrophoresis (DNA‐native‐PAGE) nuclease assay system. The level of the endonuclease activity in cell lysates was significantly decreased at certain stage by treatment of the cells with cycloheximide. However, the enzyme activity consistently remained for over 12 hours in the isolated nuclei of the apoptotic IM9 cells. The Mg2+‐dependent endonuclease isolated from the nuclei by native‐PAGE elution was able to catalyze the conversion of supercoiled plasmid DNA into linear form. This particular endonuclease activity was not detected in cycloheximide treated‐U937 cells. Several lines of experimental evidence suggest that the Mg2+‐dependent endonuclease localized in the nucleus may be responsible for the DNA fragmentation of apoptotic IM9 cells.
Factors influencing the levels of fatty acid synthase complex activity in fowl
Authors: Li Mei , Shi Yan , Tian Weixi 
Published: July 2007

AbstractThere is a notable discrepancy between the FAS (fatty acid synthase) activity of four types of fowl (egg chicken, meat chicken, egg duck, and meat duck) with distinctively different body fat levels. There is a 14.8 fold difference per unit body weight between the maximum and minimum FAS activities. The three major factors affecting this discrepancy are liver weight per unit body weight, which is 2.3 times greater in meat ducks than in egg chickens, the amount of FAS protein per gram of liver. which is 1.85 times greater in meat ducks than in egg chickens, and the FAS specific activity in meat ducks, which is 3.5 times greater in meat ducks than in egg chickens. Within the same species of egg chickens, the abdomen fat per kg of body weight at 470 days after egg production is 66 times greater than 90 days before egg production and the liver FAS activity is increased 9.6 fold. The 9.6 fold FAS activity increase resulted from an increase in the specific activity, since the liver weight per kilogram of body weight remained constant at approx. 20 grams and the FAS weight per gram of liver also remained constant at approx. 4.5rag. This shows that the control of the basic FAS activity level which is closely related to the level of body fat does not mainly arise from genetic control. For the same kind of fowl, the control of the basic FAS activity level occurs after gene expression. It is suggested that control may be imposed in the folding phase when new peptides give rise to functional proteins.
Relationship between the physicochemical parameters and biological activity of sulfosuccinic and ester surfactants
Authors: Oros Gyula , Cserhàti Tibor , Vrbanovà Alena 
Published: July 2007

AbstractThe effect of 11 sulfosuccinic acid ester surfactants on the abiotrophic developmental forms of the asexual spores of Plasmopara halstedii was determined. The strength and the selectivity of the effect was separated by the spectral mapping technique and the relationship between the biological activities and physicochemical parameters of anionic surfactants was elucidated by stepwise regression analysis. It was established that the plasma‐lemma of cell wall less zoospores showed the highest and the resting zoosporangia the lowest average sensitivity toward the surfactants. The biological efficacy of surfactants was highly different the di‐n‐octyl ester being the most effective. Calculations proved that both the stregth and selectivity of the biological activity mainly depends on the lipophilicity of the molecule and, to a lesser extent, on the electronic parameters.
The polarization model in bacterial photosynthesis
Authors: Borisov A.Yu. , Fok M. V. 
Published: July 2007

AbstractThe widely accepted model of reaction center /RC/ functioning is proved to come into contradiction with some recent data. In particular, it cannot explain why only a minor part of electronic excitations (≈︁10%) escapes from excited RC special pairs back to antenna BChls. Therefore we believe that the model must be substantially modernized. In 1981 we developed a new model/1,2/. We suggested a femtosecond state to precede primary e‐transfer reaction due to reorientation of water molecule dipole in the electric field of excited RC dimer. This mechanism is responsible for energy trapping before the primary e‐transport occurs. During last years his mechanism got support from various experimental works. Now this polarization model claims to fit all reliable experimental data at least in bacterial photosynthesis.
Differential regulation of protooncogene c‐myc expression in rat ventral prostate after castration
Authors: Lee Joong‐Hwa , Sul Chong‐Koo , Kim Young‐Kyun , Hwang Byung‐Doo , Lim Kyu 
Published: July 2007

AbstractTo evaluate the possibility that the protooncogene c‐myc plays a role in ventral prostate, the effects of castration have been investigated at a beginning of a period by Northern blot hybridization and the levels of c‐myc mRNA were also compared with mRNA of androgen‐regulated genes, C1 and TRPM‐2. Levels of c‐myc mRNA in ventral prostate increased with maximal stimulation reached at 6 hours (early induction) and 48 hours (late induction) after castration, respectively. The level of C 1 mRNA did not change and TRPM‐2 was not detected at early induction of c‐myc mRNA after castration. The level of early induction of c‐myc mRNA after castration was increased in ventral prostate treated with cycloheximide, but it was almost reduced by actinomycin‐D pretreatment. Administration of androgen at the time of castration prevented early induction of c‐myc mRNA. These results suggest that protooncogene c‐myc is differentially regulated in ventral prostate after castration.
Pre‐conditioning to global cerebral ischemia changes hippocampal acetylcholinesterase in the rat
Authors: Schetinger Maria R. C. , Bonan Carla D. , Frassetto Silvana S. , Wyse Angela T. S. , Schierholt Rejane C. , Webber Analupe , Dias Renato D. , Sarkis Jaão J. F. , Netto Carlos A. 
Published: July 2007

AbstractThis study shows the effect of transient global cerebral ischemia (ISC) on hippocampal acetylcholinesterase (AChE) activity. Naive adult Wistar rats received either a brief (2 min) or a long (10 min) ischemic episode by the four‐vessel occlusion method. Pre‐conditioned rats received double ischemia: a 10 min episode inflicted 24 h after a 2 min event, a condition known to confer cytoprotection to CA1 pyramidal cells of hippocampus. 2 min of ischemia caused an increase in acetylcholinesterase activity both immediately and 30 min after the episode, however enzyme activity was significantly decreased after 24 h of reperfusion. 10 min of ischemia caused an increase in activity both 60 min and 24 h after ischemia. Conversely, pre‐conditioned rats displayed lower activity both immediately and 60 min after ischemia. Our results suggest that: a) neuronal death, that follows 10 min of ischemia, is associated to a late increase in acetylcholinesterase activity; b) pre‐conditioning is related to diminished acetylcholinesterase activity. This is in agreement with previous evidence that acetylcholinesterase inhibition and maintenance of acetylcholine levels are beneficial for cell surviving after cerebral ischemia.