bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Aerobic Exercise on Cardiac Inflammatory Cytokines, Apoptotic Pathways, and Myocardial Preservation in a Rat Model of Type 2 Diabetes Mellitus
Authors: Rashidpour¹ Alireza , Piralaiy² Elaheh , Ismael¹ Badrkhan Rashwan , Hamidian³ Gholamreza , Naddafha⁴ Siavash 
Published: February 2025

Abstract

Background
Type 2 diabetes mellitus (T2DM) is associated with an increased risk of cardiovascular complications, partially mediated by chronic inflammation and myocardial injury. This study aimed to investigate the Aerobic Exercise Reduces Cardiac Inflammation and Apoptosis in Diabetic Rats.
Methods
A total of 20 male Wistar rats (average weight: 240 ± 28 g) were randomly divided into four groups: diabetic exercise (Dia + Exe), healthy exercise (Heal + Exe), diabetic control (Dia + Con), and healthy control (Heal + Con). The Dia + Exe and Dia + Con groups were fed a diet consisting of 60% high-fat food for a specified duration before receiving intratracheal injections of streptozotocin to induce diabetes. The Dia + Exe and Heal + Exe groups underwent aerobic exercise on a treadmill at speeds ranging from 5 to 24 meters per minute for eight weeks. Levels of TNF-α and IL-6 were measured using ELISA, while caspase-3 activity was assessed via real-time PCR.
Results
Compared to the Heal + Con group, the diabetic control group (Dia + Con) displayed a notable elevation in TNF-α, IL-6, and caspase-3 levels (P ≤ 0.05), indicative of heightened inflammation and apoptosis. Conversely, the diabetic exercise group (Dia + Exe) that underwent aerobic exercise demonstrated a reduction in TNF-α, IL-6, and caspase-3 levels compared to the Dia + Con group (P ≤ 0.05).
Conclusion
The results of this study demonstrated that aerobic exercise could reduce inflammatory markers such as TNF-α, IL-6, and caspase-3, particularly in cardiac tissue. These findings underscore the potential of aerobic exercise as a non-pharmacological strategy to mitigate cardiac complications in diabetic patients.
Constructing synthetic nuclear architectures via transcriptional condensates in a DNA protonucleus
Authors: Walther Andreas , Xie Miao , Chen Weixiang , Roy Maria 
Published: February 2025

Abstract

Nuclear biomolecular condensates are essential sub-compartments within the cell nucleus and play key roles in transcription and RNA processing. Bottom-up construction of nuclear architectures in synthetic settings is non-trivial but vital for understanding the mechanisms of condensates in real cellular systems. Here, we present a facile and versatile synthetic DNA protonucleus (PN) platform that facilitates localized transcription of branched RNA motifs with kissing loops (KLs) for subsequent condensation into complex condensate architectures. We identify salinity, monomer feeding, and KL-PN interactions as key parameters to control co-transcriptional condensation of these KLs into diverse artificial nuclear patterns, including single and multiple condensates, interface condensates, and biphasic condensates. Over time, KL transcripts co-condense with the PN matrix, with the final architecture determined by their interactions, which can be precisely modulated using a short DNA invader strand that outcompetes these interactions. Our findings deepen the understanding of RNA condensation in nuclear environments and provide new strategies for designing functional nucleus-mimetic systems with precise architectural control.
Engineered virus-like particle-assembled VEGF-targeting Cas9 ribonucleoprotein treatment alleviates neovascularization in wet age-related macular degeneration
Authors: Wu Jun , Jang Hyewon , Kwak Hyunjong , Lee Seok Jae , Jo Dong Hyun , Kim Hyongbum Henry , Kim Jeong Hun 
Published: February 2025

Abstract

Background
Age-related macular degeneration (AMD), particularly the wet form, is a leading cause of vision loss, characterized by abnormal blood vessel growth in the retina. Targeting vascular endothelial growth factor (VEGF) has proven effective in mitigating the disease. While knock-out VEGF expression using genome editing is a proven therapeutic strategy, conventional delivery systems such as adeno-associated virus (AAV) and lipid nanoparticles (LNPs) face limitations, including prolonged expression, potential genome integration, and reduced efficiency for macromolecule delivery. Engineered virus-like particles (eVLPs) offer an alternative, combining the efficiency of viral systems with the transient nature of non-viral platforms. Here, we investigated the therapeutic efficacy of eVLPs for the transient delivery of VEGF-targeting Cas9 ribonucleoprotein (RNP) in a laser-induced choroidal neovascularization (LI-CNV) mouse model of wet AMD.
Methods
EVLPs were produced by co-transfecting HEK293T cells with plasmids encoding VSV-G, MMLVgag–pro–pol, MMLVgag–3xNES–Cas9, and gN19-Vegfa sgRNA. The delivery efficiency and VEGF disruption were initially assessed in NIH/3T3 cells through immunocytochemistry, enzyme-linked immunosorbent assay, and deep sequencing. In vivo, Cas9-eVLPs were administered to the LI-CNV mouse model to evaluate the delivery efficiency and therapeutic efficacy. Insertion and deletion (INDEL) frequency, VEGF expression change, therapeutic efficacy, and safety issus were evaluated using deep sequencing, ELISA, histological analyses, and ocular functional tests.
Results
Cas9-eVLPs demonstrated efficient intracellular delivery in vitro, achieving up to 99% indel frequency at the VEGF target locus and significant VEGF downregulation without cytotoxicity. Single subretinal injection of Cas9-eVLPs into the retinal pigment epithelium (RPE) effectively disrupted VEGF, with expression nearly abolished by day seven. In LI-CNV mouse model, this intervention reduced CNV area and volume by 32.9% and 26.5%, respectively, while significantly decreasing VEGF protein levels in the RPE. Furthermore, Cas9-eVLPs delivery did not cause any retinal anatomical or functional toxicity.
Conclusion
This study highlights the potential of eVLPs as a safe and efficient delivery platform for Cas9 RNPs, achieving precise VEGF knockout and significant reductions in neovascularization in a mouse model of wet AMD. With transient Cas9 expression, high editing efficiency, and minimal risk of genomic integration, eVLPs present a promising alternative to conventional delivery systems for advancing genome editing therapies.
Multisystem Inflammatory Syndrome in Children associated with COVID-19 – A Case Report and Literature Review.
Authors: Ebrahim Mohamedraza Akbar , Makundi Shadrack Elia , Moshi Noureen Ndelimbi , Ibrahim Pendo Mussa , Osman Rukhsar Shabir , Fidaali Zainab , Nkya Deogratius Arnold 
Published: February 2025

Abstract

Given the rarity of Multi-system Inflammatory Syndrome related to COVID-19 (MIS-C). This case report highlights the high index of suspicion to diagnose MIS-C in a child who presented with symptoms suggestive of acute gastroenteritis. While admitted to the hospital, her condition deteriorated, with worsening symptoms, clinical decline, deranging laboratory markers and significant radiological findings even with antibiotics. She had acute myocarditis with myocardial dysfunction on echocardiography and increased cardiac enzymes. Intravenous human immunoglobulin (IVIG) was given, but there was an additional need for inotropes (norepinephrine) and methylprednisolone, although the preliminary echocardiogram was normal. Early recognition of MIS-C with rapid escalation of care after IVIG administration is needed to prevent morbidity and mortality.
Phytochemical Analysis of Selected Indigenous Wild Edible Fruits Used by the Kattunaikka Tribe of Wayanad District, Kerala, India
Authors: Abdussalam AK , Prasanth KP , Azeez K , Prajith PK 
Published: February 2025

Wayanad district, located in the northeastern part of Kerala state, features hilly terrain within the southern Western Ghats. The district boasts impressive ethnic diversity, with ten different tribal groups. Among these, the Kattunaikka tribe is particularly significant for their conservation and consumption of wild edible fruits. Fruit crops have been a part of our culture for centuries. Ethnic people all over the world have relied on a variety of wild food plants to meet their nutritional and dietary needs. Fruits have a significant role in human diets. They meaningfully contribute to human well-being in terms of nutrition because of their great nutritional value. Seasonal surpluses of perishable fruits are readily available. Among the wild foodstuff, wild edible fruits form a major part of diet supplements, especially for rural people. Large quantities of agricultural produce go to waste in specific locations during particular seasons due to a combination of factors, including ignorance and a lack of management skills in distribution, marketing, and storage. To spread the word about nutritional and therapeutic benefits of these fruits, present investigation has been carried out. The selected wild edible fruits, such as Tamilnadia uliginosa, Diplocyclos palmatus, Momordica dioica, Physalis minima and Syzygium caryophyllatum were collected from different hamlets of Kattunaikk tribes of Wayanad District, northern Kerala at the time of ripening. Several factors were similar, including how moisture, dry matter, carotenoids and carbohydrates were assessed. Additionally, antioxidant enzymes such as superoxide dismutase, peroxidase, and catalase were thoroughly analyzed. Important metabolites like reducing sugar, starch, and soluble sugar are estimated with standard procedure. Selected five wild fruits are considered edible and commonly used by rural people in Northern Kerala.
Geospatial Modeling of Carbon Emission Reduction Achievement in Siak Regency, Riau Province
Authors: Putri Amaliyah , Purwanto Ris Hadi , Soraya Emma 
Published: February 2025

The Siak Regency implemented the Green Siak Policy in 2016 to commit to reducing carbon emissions. This research aimed to assess land use and land cover (LULC) changes from 2016 to 2023 and make projections for 2030, quantify carbon stocks by LULC type, and estimate CO₂ emissions associated with the implementation of the Green Siak Policy. This research classified LULC using Landsat imagery. It employed the CA-Markov to project land cover in 2030 using eight driving factors: elevation, temperature, rainfall, population density, distance from roads, burned areas, state forest areas, and evidence likelihood. This research assessed carbon stocks using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model and calculated CO₂ emissions based on changes in LULC and peat decomposition. The findings revealed a slight reduction in total carbon stock from 1,232.52 MtC in 2016 to 1,232.12 MtC in 2023, with annual CO₂ emissions of 1.4 MtCO₂e. Projections indicated an increase in carbon stock, expected to reach 1,232.27 MtC by 2030, with anticipated annual emissions of 1.398 MtCO₂e from 2023 to 2030. While the Green Siak Policy targeted a decrease in emissions of 23.28 MtCO₂e/year by 2030, the results indicated that the Regency achieved merely 0.03% of its target.
Pelatihan Pengelolaan Media Sosial Dalam Meningkatkan Pemasaran Online Olahan Produk Nanas Pada Usaha Mikro Desa Tangkit Baru
Authors: Wediawati Besse , Setiawati Rike , Amin Shoifa , Widiastuti Fitri , Ratnawati RTS 
Published: February 2025

Pemasaran online merupakan suatu aktivitas yang tidak dapat diabaikan di era bisnis digital bagi uusaha Mikrokecil sebagai suatu strategi untuk memperluas jaringan pemasaran mengunakan berbagai media digital/sosial. Kegiatan Pengabdian ini bertujuan membantu memecahkan problem rendahnya literasi digital dan masalah pemasaran uusaha Mikroyang masih terbatas karena masih mengandalkan pemasaran konvensional (offline). Solusi yang dilakukan adalah fasilitasi pelatihan literasi digital dan pemasaran melalui media sosial (0nline). Mitra peserta pelatihan ini adalah kelompok Usaha pengolahan nenas di Desa Tangkit Baru yaitu Kelompok Abadi Group sebanyak 10 Usaha mikro. Metode yang digunakan adalah metode ceramah, diskusi/tanyajawab, demonstrasi/simulasi serta praktek langsung dalam pembuatan dan editing foto dan video serta memposting ke media sosial yang relevan bagi SAHA MIKROuntuk pemasaran online mereka. Luaran kegiatan ini berupa meningkatnya literasi digital terkait pemanfaatan media sosial untuk memperluas akses pemasaran online di kelompok mitra.
Å dyrke dyden: nyaristoteliske konsepter i utviklingen av det offentlige rom
Authors: Sirowy Beata , Knight Kelvin 
Published: February 2025

In this chapter we demonstrate how the insights from contemporary Aristotelian ethical and political thought might inform urban planning and challenge its utilitarian norms. We propose the Aristotelian concepts of eudaimonia—which we translate as human well-being or flourishing – and of the virtues (excellences of character and understanding) and civic friendship as guiding concepts for today’s urban development, especially in the design and programming of urban public space.
In determining how best to integrate the above-mentioned notions in urban development, we employ the neo-Aristotelian concept of practices, as distinct from organizational institutions, and introduce the concept of communities of virtue proposed by Alasdair MacIntyre. We argue that the development of urban public space should take account of citizens’ participative practices, rather than only of functions that are administratively conceived.
We conclude with a discussion of urban agriculture as an example of a practice situated in urban public space – a cooperative activity that cultivates the ethical virtues, sociality, and productive skills of its participants in pursuit of a common good, whilst also contributing to the wider good of local communities.
Edukasi 3R dalam Penanganan Sampah Menuju Sekolah Adiwiyata
Authors: Sari Febrina , Abdillah Nuryasin , Desyanti  
Published: February 2025

Permasalahan utama adalah penumpukan sampah yang semakin meningkat di lingkungan sekolah. Sampah organik dan anorganik tidak terkelola dengan baik, siswa masih membuang sampah organik dan anorganik menjadi satu di tempat sampah yang sama sehingga menyebabkan penumpukan sampah. Tujuan dari kegiatan ini adalah Tujuan penelitian ini untuk menganalisis edukasi 3R dalam penanganan sampah menuju sekolah adiwiyata SD. Rangkaian kegiatan untuk menyelesaikan permasalahan mitra dilaksanakan dalam beberapa tahapan mulai dari sosialisai, pelatihan dan penerapan iptek. Hasil penelitian yaitu kegitan ini menyediakan sarana prasarana pengelolaan sampah yang mendukung, seperti tempat sampah terpisah untuk sampah organik dan anorganik, dengan adanya kegiatan ini maka siswa dapat memilah sampah dan membuang sampah sesuai dengan jenisnya, mengajarkan siswa untuk meningkatkan kebersihan lingkungan. Dampak dari kegiatan ini adalah berkurangnya sampah yang berserakan di lingkungan sekolah karena sudah ada tempat sampah yang sesuai dengan jenis sampah, meningkatnya kesadaran siswa untuk menjaga lingkungan sekolah agar tetap bersih dan bebas dari sampah. Implikasi penelitian ini yaitu mitra telah memiliki pengetahuan dan keterampilan dalam mengelolah sampah yang ada di sekolah dengan metode 3R, sehingga sampah memiliki nilai juga.
Penguatan Hospitality dan Interpretasi Daya Tarik Wisata Berbasis Sport Edutourism di Objek Wisata
Authors: Kartika Diana , Mulatsih Listiana Sri , Astuti Yuni , Maharani Jelita , Fikri Muhammad , Haryanto Bayu , Almauli Rizki 
Published: February 2025

Salah satu ikon pariwisata unggulan menghadapi tantangan dalam pengelolaan destinasi wisata yang berkelanjutan dan peningkatan kualitas sumber daya manusia (SDM) di sektor pariwisata. Sehingga, diperlukan pendekatan konsep sustainable tourism berbasis sport edutourism untuk mengoptimalkan tata kelola objek wisata dan meningkatkan kapasitas SDM lokal. Tujuan pengabdian ini untuk mengembangkan SDM kepariwisataaan yang mampu menerapakan hospitality dan berkomunikasi dengan efektif untuk menjelaskan interpretasi daya tarik wisata yang ada di objek wisata. Metode yang digunakan dalam penelitian ini adalah HEI (Hospitality, Edukatif, Informatif) dengan peserta 25 orang yang berasal dari Pokdarwis, pelaku wisata dan kelompok masyarakat Data dikumpulkan melalui wawancara, observasi, dan studi dokumentasi dengan instrumen berupa panduan wawancara, lembar observasi, dan kuesioner. Analisis data menggunakan teknik deskriptif kualitatif dan kuantitatif. Hasil penelitian menunjukkan peningkatan keterampilan hospitality, komunikasi, dan interpretasi wisata peserta yang sejalan dengan tujuan penelitian. Kesimpulan penelitian ini adalah pemberdayaan berbasis CBT efektif untuk meningkatkan kompetensi SDM pariwisata yang profesional dan mandiri. Implikasi penelitian ini dapat mendukung pengembangan Gunuang Padang sebagai destinasi edukasi dan konservasi berkelanjutan yang berkontribusi terhadap pencapaian SDGs.
Comparison of annual electrical energy losses of conventional and superconductive transformers in power lines
Authors: Wajda Józef 
Published: February 2025

The aim of the work is to compare performance and annual results losses of electrical energy lost by transformers superconducting and conventional. The efficiency has been calculated both transformers installed in the power plant at changing load. The example used superconducting transformer with 2G0 winding. Obtained the results show 80% less energy loss per year compared to transformers with copper windings.
Visual metonymy of boy parts in Krzysztof Kieślowski’s “Three colours: Blue” (1993), A pilot study
Authors: Karasová Lucia 
Published: February 2025

The study investigates the role of cognitive tools of PART–FOR–WHOLE or SOURCE–IN–TARGET conceptual metonymy and image schemas inherent in framing in highlighting characters’ inner states, emotions, intentions and actions. The study analyses each shot containing a close-up/extreme close-up of a body part in Krzysztof Kieślowski's "Three Colours: Blue" (1993). It further validates research conducted by cognitive film linguists like Coëgnarts (2019a, 2019b), and Coëgnarts & Kravanja (2016a, 2016b, 2016c) who claim that framing techniques elicit image schemas that shape the viewer's perception of the onscreen reality. It is argued that conceptual metonymy is employed to highlight characters’ mental states and emotions through body parts and their actions to enable the viewer to identify with the characters’ inner states, which the analysis offered confirms.
Dining Preferences of Mall-Goers in Bohol
Authors: Cabig Hersheylou 
Published: February 2025

The study is to assess the level of preference in dining establishments of mall-goers in Bohol. Specifically, the study sought to answer the respondent’s profile in terms of the age, gender, nationality and income. Moreover, the perceived level of preference of mall-goers and problems encountered during their dining experience, the significant relationship and difference between their profiles and level of preference were assessed. In the views thereof, the study would propose a program to retain customer satisfaction and loyalty in the service offered by establishment.
This study utilized descriptive-correlational method of research using a survey questionnaire to determine the level of preference of customer in dining establishments. The sample size was 50 mall-goers using purposive sampling. The locale of the survey was in Tagbilaran City as a hub of commerce and lone city of the province of Bohol where we can find the Island City Mall. The survey used a researcher made survey questionnaire which was composed of three parts: profile, level of preference of mall-goers in terms of food variation and taste, service personnel, price and ambience based on the Likert rating scale. Treatment of data were done using frequency count and per cent distribution; weighted mean and ranking; regression; and one-way ANOVA.
Results showed that most of the respondents were young adults with ages below 20 years old, mostly were females and Asian with below P10, 000.00 income. Furthermore, it showed that customers have problems with the staffs because of their slow and not good service. This amplified the hypothesis that there is a significant relationship between the respondent’s profiles in terms of age, gender, and nationality with their level of preference, and there is a significant difference between the respondent’s profile in terms of income and their level of preference. It was concluded that respondents highly preferred all of the attributes in a dining establishment in terms of food variation and taste, service personnel, price, and ambience. Finally, with the findings of the study, it was recommended that management should conduct seminars and trainings for job enrichment, motivational patterns and goal consciousness for food and drink provision.
Mental Well-being and Life Satisfaction as the Outcomes of Life Orientation and Resilience among University Students
Authors: Saleh Fatima , Chattha Ali Haider , Salma Hasan Syeda 
Published: February 2025

The purpose of this study was to investigate into how life orientation and resilience affect university students' sense of life satisfaction and mental health. It was hypothesized that the correlation between life orientation, resilience, mental health, and life satisfaction would be statistically significant. It was also hypothesized that resilience and life orientation would be significant predictors of life satisfaction and mental-wellbeing, and mental well-being and life satisfaction would have significant differences in terms of gender. The study's sample (N=300) was drawn from university students. Purposive sampling was employed to choose all participants, with age ranging from 18 to 29 years. The Life Orientation Scale (1994) developed by Scheier, the Resilience Scale (NMRQ) developed by McBride (2020), the Warwick Edinburgh Mental Well-being Scale (2006), and the Life Satisfaction Scale (SWLS) developed by Deiner (1985) were used to assess university the study variables. The current study showed that life satisfaction, resilience, life orientation, and mental well-being are all significantly positively correlated. The results also revealed that resilience and life orientation strongly predict the mental health of university students. Life orientation was found to be a significant predictor of university students' life satisfaction. Male university students scored higher than female university students in terms of mental health and life satisfaction.