bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drought priming‐induced stress memory improves subsequent drought or heat tolerance via activation of γ‐aminobutyric acid‐regulated pathways in creeping bentgrass
Authors: Yuan Y. , Tan M. , Zhou M. , Hassan M. J. , Lin L. , Lin J. , Zhang Y. , Li Z. 
Published: March 2024

Abstract
Recurrent drought can induce stress memory in plants to induce tolerance to subsequent stress, such as high temperature or drought. Drought priming (DP) is an effective approach to improve tolerance to various stresses; however, the potential mechanism of DP‐induced stress memory has not been fully resoved.
We examined DP‐regulated subsequent drought tolerance or thermotolerance associated with changes in physiological responses, GABA and NO metabolism, heat shock factor (HSF) and dehydrin (DHN) pathways in perennial creeping bentgrass.
Plants can recover after two cycle of DP, and DP‐treated plants had significantly higher tolerance to subsequent drought or heat stress, with higher leaf RWC, Chl content, photochemical efficiency, and cell membrane stability. DP significantly alleviated oxidative damage through enhancing total antioxidant capacity in response to subsequent drought or heat stress. Endogenous GABA was significantly increased by DP through activating glutamic acid decarboxylase activity and inhibiting GABA transaminase activity. DP also enhanced accumulation of NO, depending on NOS activity, under subsequent drought or heat stress. Transcript levels of multiple transcription factors, heat shock proteins, and DHNs in the HSF and DHN pathways were up‐regulated by DP under drought or heat stress, but there were differences between DP‐regulated heat tolerance and drought tolerance in these pathways.
The findings indicate that under recurrent moderate drought, DP improves subsequent tolerance to drought or heat stress in relation to GABA‐regulated pathways, providing new insight into understanding of the role of stress memory in plant adaptation to complex environmental stresses.
The Glycine soja cytochrome P450 gene GsCYP82C4 confers alkaline tolerance by promoting reactive oxygen species scavenging
Authors: Wu Jinyu , Fang Yangyang , Xu Liankun , Jin Xiaoxia , Iqbal Anam , Nisa Zaib_un , Ali Naila , Chen Chao , Shah Anis Ali , Gatasheh Mansour K. 
Published: March 2024

AbstractRecent studies have demonstrated the crucial role of Cytochrome P450 enzymes (CYPs) in the production of secondary metabolites, phytohormones and antioxidants in plants. However, their functional characterization specifically under alkaline stress remains elusive. CYP82C4 was the key gene screened from a family of wild soybean CYPs in our previous studies. The aim of this present study was to clone the Glycine soja GsCYP82C4 gene and characterize its functions in Arabidopsis and Glycine max. The results showed that the GsCYP82C4 gene displayed a high expression in different plant tissues at mature stages compared to young stages. Further, higher temporal expression of the GsCYP82C4 gene was noted at 6, 12 and 24 h time points after alkali treatment in leaves compared to roots. In addition, overexpression of GsCYP82C4 improved alkaline stress tolerance in Arabidopsis via increased root lengths and fresh biomass and strengthened the antioxidant defense system via a reduction in superoxide radicals in transgenic lines compared to wild type (WT) and atcyp82c4 mutants. Further, the expression levels of stress‐related marker genes were up‐regulated in GsCYP82C4 OX lines under alkali stress. The functional analysis of GsCYP82C4 overexpression in soybean displayed better hairy root growth, increased fresh weight, higher antioxidant enzyme activities and reduced lipid peroxidation rates in OX lines compared to the soybean WT (K599) line. In total, our study displayed positive roles of GsCYP82C4 overexpression in both Arabidopsis and Glycine max to alleviate alkaline stress via altering expression abundance of stress responsive genes, stronger roots, higher antioxidant enzyme activities as well as reduced rates of lipid peroxidation and superoxide radicals.
Coworking Spaces and Mid‐Sized Cities in Peripheral Contexts: Conceptualising Development Trajectories
Authors: Danko Lukáš , Bednář Pavel , Lux Gabor , Kalman Judit , Belvončíková Eva , Horeczki Réka , Bálint Dóra 
Published: March 2024

AbstractCoworking spaces and the creative industries represent a rapidly growing but highly diverse shared economy sector. The paper outlines the types of incremental or radical forms of change developed that lead to industrial–institutional coevolution based on evidence from selected cities in Central and Eastern Europe. The results suggest that CS in peripheral areas are contributing primarily to the path upgrading through local embeddedness of stakeholders. These spaces contribute to refocusing local creative ecosystems by enhancing competitiveness of microclusters. Results suggest that CS in peripheral areas are contributing primarily to the path upgrading through local embeddedness of stakeholders through remote workers and digital nomads These cases underline replicative entrepreneurship, where local stakeholders gather momentum for a subsequent period characterised by new internal structures and services in the urban setting. Considering the form of change, our results emphasise the importance of reproductive agency based on diverse services to digital nomads and remote workers.
Near‐infrared imaging of phytochrome‐derived autofluorescence in plant nuclei
Authors: Yoshinari Akira , Isoda Reika , Yagi Noriyoshi , Sato Yoshikatsu , Lindeboom Jelmer J. , Ehrhardt David W. , Frommer Wolf B. , Nakamura Masayoshi 
Published: March 2024

SUMMARYCapturing images of the nuclear dynamics within live cells is an essential technique for comprehending the intricate biological processes inherent to plant cell nuclei. While various methods exist for imaging nuclei, including combining fluorescent proteins and dyes with microscopy, there is a dearth of commercially available dyes for live‐cell imaging. In Arabidopsis thaliana, we discovered that nuclei emit autofluorescence in the near‐infrared (NIR) range of the spectrum and devised a non‐invasive technique for the visualization of live cell nuclei using this inherent NIR autofluorescence. Our studies demonstrated the capability of the NIR imaging technique to visualize the dynamic behavior of nuclei within primary roots, root hairs, and pollen tubes, which are tissues that harbor a limited number of other organelles displaying autofluorescence. We further demonstrated the applicability of NIR autofluorescence imaging in various other tissues by incorporating fluorescence lifetime imaging techniques. Nuclear autofluorescence was also detected across a wide range of plant species, enabling analyses without the need for transformation. The nuclear autofluorescence in the NIR wavelength range was not observed in animal or yeast cells. Genetic analysis revealed that this autofluorescence was caused by the phytochrome protein. Our studies demonstrated that nuclear autofluorescence imaging can be effectively employed not only in model plants but also for studying nuclei in non‐model plant species.
Microbiota of laboratory channel catfish skin mucosa and aquaria water exposed to chloramine-T trihydrate
Authors: Kittel Elliott , Crosby Tina , Kocurek Brandon , Ottesen Andrea , Gieseker Charles 
Published: March 2024

ABSTRACT

Here, we describe the skin mucosa microbiome of channel catfish (
Ictalurus punctatus
) before and after exposure to chloramine-T trihydrate. We also describe the aquaria water microbiome after the post-treatment period. These data provide a unique baseline description of skin mucosa and aquaria water microbiome from catfish reared in research aquaria.
Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation
Authors: Gager Christopher , Flores-Mireles Ana L. 
Published: March 2024

ABSTRACT

The spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a powerful component of microbial pathogenesis, providing a scaffold for efficient colonization and shielding against anti-microbials, which further complicates drug resistance studies. Early genetic knockout tools didn’t allow the study of essential genes, but clustered regularly interspaced palindromic repeat inference (CRISPRi) technologies have overcome this challenge via genetic silencing. These tools rapidly evolved to meet new demands and exploit native CRISPR systems. Modern tools range from the creation of massive CRISPRi libraries to tunable modulation of gene expression with CRISPR activation (CRISPRa). This review discusses the rapid expansion of CRISPRi/a-based technologies, their use in investigating MDR and biofilm formation, and how this drives further development of a potent tool to comprehensively examine multi-drug resistance.
Skin-to-skin stabilisation and uninterrupted respiratory support for preterm infants after birth: feasibility of a new and simplified rPAP system
Authors: Baldursdottir Sonja , Gunnarsdottir Kolbrun , Donaldsson Snorri , Jonsson Baldvin , Drevhammar Thomas 
Published: March 2024

BackgroundThe rPAP respiratory support system, used for delivery room stabilisation with nasal prongs, has been shown to reduce the need for intubation in extremely preterm infants. A simplified version of the system has been developed. The purpose of this study was to determine the feasibility of providing uninterrupted respiratory support with the simplified rPAP from birth up to 4 hours of life and to assess ease of use for skin-to skin stabilisation.MethodsThis was a non-randomised feasibility study conducted at Karolinska University Hospital, Sweden. Respiratory support with continuous positive airway pressure (CPAP) and positive pressure ventilation if needed was given with the simplified rPAP using heated humidified gases. Respiratory support was provided in the delivery room, during transportation and in the neonatal unit, for a maximum of 4 hours.Results32 preterm infants with a mean (SD) gestational age of 33.4 weeks (±1.2) were included. Of 17 infants born vaginally, 13 were stabilised skin-to-skin. The remaining infants were stabilised on a resuscitation table. All infants received CPAP and nine received positive pressure ventilation. 31 infants received continued support during transport and after arrival in the neonatal unit. Minor interruptions in CPAP support occurred in all infants. The study did not reveal problems with usability of the system.ConclusionIt is feasible to stabilise preterm infants with the simplified respiratory support system both skin-to-skin and on a resuscitation table, and to provide continued respiratory support with the same system during transportation and in the neonatal unit.Trial registration numberNCT04244890.
Real-world application of a smartphone-based visual acuity test (WHOeyes) with automatic distance calibration
Authors: Wu Yi , Keel Stuart , Carneiro Vera Lúcia Alves , Zhang Shiran , Wang Wei , Liu Chi , Tang Xuanzhang , Han Xiaotong , He Mingguang 
Published: March 2024

Background
To develop and assess the usability of a smartphone-based visual acuity (VA) test with an automatic distance calibration (ADC) function, the iOS version of WHOeyes.

Methods
The WHOeyes was an upgraded version with a distinct feature of ADC of an existing validated VA testing app called V@home. Three groups of Chinese participants with different ages (≤20, 20–40, >40 years) were recruited for distance and near VA testing using both an Early Treatment Diabetic Retinopathy Study (ETDRS) chart and the WHOeyes. The ADC function would determine the testing distance. Infrared rangefinder was used to determine the testing distance for the ETDRS, and actual testing distance for the WHOeyes. A questionnaire-based interview was administered to assess the satisfaction.

Results
The actual testing distance determined by the WHOeyes ADC showed an overall good agreement with the desired testing distance in all three age groups (p>0.50). Regarding the distance and near VA testing, the accuracy of WHOeyes was equivalent to ETDRS. The mean difference between the WHOeyes and ETDRS ranged from −0.084 to 0.012 logMAR, and the quadratic weighted kappa (QWK) values were >0.75 across all groups. The test–retest reliability of WHOeyes was high for both near and distance VA, with a mean difference ranging from −0.040 to 0.004 logMAR and QWK all >0.85. The questionnaire revealed an excellent user experience and acceptance of WHOeyes.

Conclusions
WHOeyes could provide accurate measurement of the testing distance as well as the distance and near VA when compared to the gold standard ETDRS chart.
Relationships between sodium, fats and carbohydrates on blood pressure, cholesterol and HbA1c: an umbrella review of systematic reviews
Authors: Breeze Penny , Sworn Katie , McGrane Ellen , Abraham Sarah , Cantrell Anna 
Published: March 2024

BackgroundThe relationship between nutrition and health is complex and the evidence to describe it broad and diffuse. This review brings together evidence for the effect of nutrients on cardiometabolic risk factors.MethodsAn umbrella review identified systematic reviews of randomised controlled trials and meta-analyses estimating the effects of fats, carbohydrates and sodium on blood pressure, cholesterol and haemoglobin A1c (HbA1c). Medline, Embase, Cochrane Library and Science Citation Index were search through 26 May 2020, with supplementary searches of grey literature and websites. English language systematic reviews and meta-analyses were included that assessed the effect of sodium, carbohydrates or fat on blood pressure, cholesterol and HbA1c. Reviews were purposively selected using a sampling framework matrix. The quality of evidence was assessed with A MeaSurement Tool to Assess systematic Reviews 2 (AMSTAR2) checklist, evidence synthesised in a narrative review and causal pathways diagram.ResultsForty-three systematic reviews were included. Blood pressure was significantly associated with sodium, fibre and fat. Sodium, fats and carbohydrates were significantly associated with cholesterol. Monounsaturated fat, fibre and sugars were associated with HbA1c.ConclusionMultiple relationships between nutrients and cardiometabolic risk factors were identified and summarised in an accessible way for public health researchers. The review identifies associations, inconsistencies and gaps in evidence linking nutrition to cardiometabolic health.
Comprehensive quality assessment for aphasia rehabilitation after stroke: protocol for a multicentre, mixed-methods study
Authors: Harvey Sam , Stone Marissa , Zingelman Sally , Copland David A , Kilkenny Monique F , Godecke Erin , Cadilhac Dominique A , Kim Joosup , Olaiya Muideen T , Rose Miranda L , Breitenstein Caterina , Shrubsole Kirstine , O’Halloran Robyn , Hill Annie J , Hersh Deborah , Mainstone Kathryn , Mainstone Penelope , Unsworth Carolyn A , Brogan Emily , Short Kylie J , Burns Clare L , Baker Caroline , Wallace Sarah J 
Published: March 2024

IntroductionPeople with aphasia following stroke experience disproportionally poor outcomes, yet there is no comprehensive approach to measuring the quality of aphasia services. The Meaningful Evaluation of Aphasia SeRvicES (MEASuRES) minimum dataset was developed in partnership with people with lived experience of aphasia, clinicians and researchers to address this gap. It comprises sociodemographic characteristics, quality indicators, treatment descriptors and outcome measurement instruments. We present a protocol to pilot the MEASuRES minimum dataset in clinical practice, describe the factors that hinder or support implementation and determine meaningful thresholds of clinical change for core outcome measurement instruments.Methods and analysisThis research aims to deliver a comprehensive quality assessment toolkit for poststroke aphasia services in four studies. A multicentre pilot study (study 1) will test the administration of the MEASuRES minimum dataset within five Australian health services. An embedded mixed-methods process evaluation (study 2) will evaluate the performance of the minimum dataset and explore its clinical applicability. A consensus study (study 3) will establish consumer-informed thresholds of meaningful change on core aphasia outcome constructs, which will then be used to establish minimal important change values for corresponding core outcome measurement instruments (study 4).Ethics and disseminationStudies 1 and 2 have been registered with the Australian and New Zealand Clinical Trial Registry (ACTRN12623001313628). Ethics approval has been obtained from the Royal Brisbane and Women’s Hospital (HREC/2023/MNHB/95293) and The University of Queensland (2022/HE001946 and 2023/HE001175). Study findings will be disseminated through peer-reviewed publications, conference presentations and engagement with relevant stakeholders including healthcare providers, policy-makers, stroke and rehabilitation audit and clinical quality registry custodians, consumer support organisations, and individuals with aphasia and their families.
Influence of stress history on thermal conductivity of saturated fine-grained soils
Authors: Sivaprasad Arjun , Basu Prasenjit 
Published: March 2024

This research investigated variations in thermal conductivity k for normally consolidated (NC) and overconsolidated (OC) saturated fine-grained soils at different levels of vertical effective stress. A series of undrained heating tests were performed using a specially designed consolidometer with central radial heating arrangement and temperature measurements within a soil specimen. For different soils investigated in this research, k correlated well with soil consolidation characteristics (compression index λ and swelling index κ) and vertical effective stress σvʹ at both NC and OC states. However, the rates of increment of steady- and transient-state values of k ( kst and ktr, respectively) with σv′ differed from one soil to another. For OC clays, both ktr and kst increased with preconsolidation stress σc′. A set of equations were proposed to estimate both kst and ktr for saturated fine-grained soils as functions of σv′, λ, κ, and stress history. At a particular value of σvʹ, the dependence of k on overconsolidation ratio (OCR) and σc′ marked the importance of considering stress history in the determination of k. The influence of soil mineral composition on k was also explored. Quantification of soil mineralogical composition and available data on mineral thermal conductivity enabled prediction of ktr with reasonable accuracy.
Alleviating both $$H_0$$ and $$\sigma _8$$ tensions in Tsallis cosmology
Authors: Basilakos Spyros , Lymperis Andreas , Petronikolou Maria , Saridakis Emmanuel N. 
Published: March 2024

AbstractWe present how Tsallis cosmology can alleviate both $$H_0$$

H
0

and $$\sigma _8$$

σ
8

tensions simultaneously. Such a modified cosmological scenario is obtained by the application of the gravity-thermodynamics conjecture, but using the non-additive Tsallis entropy, instead of the standard Bekenstein–Hawking one. Hence, one obtains modified Friedmann equations, with extra terms that depend on the new Tsallis exponent $$\delta $$
δ
that quantifies the departure from standard entropy. We show that for particular $$\delta $$
δ
choices we can obtain a phantom effective dark energy, which is known to be one of the sufficient mechanisms that can alleviate $$H_0$$

H
0

tension. Additionally, for the same parameter choice we obtain an increased friction term and an effective Newton’s constant smaller than the usual one, and thus the $$\sigma _8$$

σ
8

tension is also solved. These features act as a significant advantage of Tsallis modified cosmology.
Virtual segmentation of a small contact HPGe detector: inference of hit positions of single-site events via pulse shape analysis
Authors: Dai W. H. , Ma H. , Zeng Z. , Yang L. T. , Yue Q. , Cheng J. P. 
Published: March 2024

AbstractExploring hit positions of recorded events can help to understand and suppress backgrounds in rare event searches. We propose a pulse shape analysis method to discriminate single-site events (SSEs) in the inner and outer layer of a small contact P-type germanium detector (HPGe). SSEs in the inner and outer layer have different pulse shape features, of which the rise time of the $$(T_{Q})$$

(

T
Q

)

and current pulse $$(T_{I})$$

(

T
I

)

are selected for discrimination. A 500 Bq Thorium-228 (Th-228) source is used to determine the boundaries between the two layers. The double escape peak events from 2614.5 keV $$\gamma $$
γ
-ray are selected as typical SSEs, their numbers in the two layers are used to calculate the volumes and shapes of those layers. Considering the statistical and systematic uncertainties, the inner layer volume is evaluated to be 47.2% ± 0.26%(stat.) ± 0.18%(sys.) ± 0.22%(sys.) of the total sensitive volume. Selecting the inner layer as the analysis volume can reduce the external background in the signal region of Ge-76 neutrinoless double beta (0$$\nu \beta \beta )$$

ν
β
β
)

decay. We use the Th-228 data to validate the inner layer model and evaluate the background suppression power in the 0$$\nu \beta \beta $$

ν
β
β

signal region $$(Q_{\beta \beta }=2039$$

(

Q

β
β

=
2039

keV). The virtual segmentation further reduces the background from the external Th-228 source by about 10%. The virtual segmentation could be used to efficiently suppress surface background like electrons from Ar-42 decay in 0$$\nu \beta \beta $$

ν
β
β

experiments using germanium detectors immersed in liquid argon.
Reversed Effect of Eccentricity: Perceived Duration of Peripherally Presented Stimuli Depends on Time Span
Authors: Krug Alina , Eberhardt Lisa Valentina , Pittino Ferdinand , Huckauf Anke 
Published: March 2024

Abstract
The perceived duration of stimuli presented in the visual periphery decreases with increasing eccentricity for brief durations. However, studies using different experimental methods or longer durations have reported inconsistent results. The present study aims to systematically explore the impact of stimulus eccentricity on perceived duration across various duration ranges. In three experiments employing the temporal bisection task, participants categorised the duration of stimuli presented at 3° or 9° of eccentricity as rather short or long, referred to two anchor durations previously learned. Stimulus durations varied between experiments and included short durations of 20–220 ms (Experiment 1), long durations of 400–1600 ms (Experiment 2), and intermediate durations of 160–660 ms (Experiment 3). For the short durations of Experiment 1, the results again replicated the decrease in perceived duration with increasing eccentricity. Interestingly, for the long durations, the effect was reversed, while subjective duration did not differ significantly between eccentricities for the intermediate durations. We discuss the findings with respect to prominent models of time perception, considering potential differences in attentional processing of short and long durations.
One Hundred Thousand Greetings!
Authors: Sheikh Hossein 
Published: March 2024

Abstract
The Judeo-Persian texts from the Afghan Ginza provide us with unique information about the Jewish communities in Central Asia before the Mongol invasion. Among these texts, some letters can be considered not only the earliest evidence of the New Persian language, but more specifically the earliest evidence of the epistolary tradition in New Persian. This paper examines the opening sections of these letters in order to demonstrate and discuss the changes and the evolution of the opening formulae over time.