bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translocation of fibroblast growth factor‐10 and its receptor into nuclei of human urothelial cells
Authors: Kosman Jeffrey , Carmean Nicole , Leaf Elizabeth M. , Dyamenahalli Kiran , Bassuk James A. 
Published: April 2007

AbstractFibroblast growth factor‐10 (FGF‐10), a mitogen for the epithelial cells lining the lower urinary tract, has been identified inside urothelial cells, despite its acknowledged role as an extracellular signaling ligand. Recombinant (r)FGF‐10 was determined by fluorescence microscopy optical sectioning to localize strongly to nuclei inside cultured urothelial cells. To clarify the possible role of a nuclear localization signal (NLS) in this translocation, a variant of rFGF‐10 was constructed which lacked this sequence. rFGF‐10(no NLS) was found in cytoplasm to a far greater degree than rFGF‐10, identifying this motif as a possible NLS. Furthermore, this variant displayed poor or non‐existent bioactivity compared to the wild‐type protein in triggering mitogenesis in quiescent urothelial cells. The presence of rFGF‐10(no NLS) in the nucleus suggested that additional interactions were also responsible for the nuclear accumulation of rFGF‐10. The FGF‐10 receptor was observed in cell nuclei regardless of the presence or concentration of exogenous rFGF‐10 ligand. Co‐localization studies between rFGF‐10 and the FGF‐10 receptor revealed a strong intracellular relationship between the two. This co‐localization was seen in nuclei for both rFGF‐10 and for rFGF‐10(no NLS), although the correlation was weaker for rFGF‐10(no NLS). These data show that an NLS‐like motif of rFGF‐10 is a partial determinant of its intracellular distribution and is necessary for its mitogenic activity. These advancements in the understanding of the activity of FGF‐10 present an opportunity to engineer the growth factor as a therapeutic agent for the healing of damaged urothelial tissue. J. Cell. Biochem. 102: 769–785, 2007. © 2007 Wiley‐Liss, Inc.
Cytoskeletal elements and intracellular transport
Authors: Bhaskar Lakshmi , Krishnan Vidya S. , Thampan Raghava Varman 
Published: April 2007

AbstractRecent advances in the understanding of the functions of various components of the cytoskeleton indicate that, besides serving a structural role, the cytoskeletal elements may regulate the transport of several proteins in the cell. Studies reveal that there are co‐operative interactions between the actin and microtubule cytoskeletons including functional overlap in the transport influenced by different motor families. Multiple motors are probably involved in the control of the dynamics of many proteins and intriguing hints about how these motors are co‐ordinated are appearing. It has been shown that some of the intermediate elements also participate in selected intracellular transport mechanisms. In view of the author's preoccupation with the steroid receptor systems, special attention has been given to the role of the cytoskeletal elements, particularly actin, in the intracellular transport of steroid receptors and receptor‐related proteins. J. Cell. Biochem. 101:1097–1108, 2007. © 2007 Wiley‐Liss, Inc.
Delayed activation of PKCδ and NFκB and higher radioprotection in splenic lymphocytes by copper (II)–Curcumin (1:1) complex as compared to curcumin
Authors: Kunwar Amit , Narang H. , Priyadarsini K. Indira , Krishna M. , Pandey R. , Sainis K.B. 
Published: April 2007

AbstractA mononuclear 1:1 copper complex of curcumin had been found to be superior to curcumin in its anti‐oxidant properties. This paper describes the radio‐protective effects of the complex in splenic lymphocytes from swiss mice. The complex was found to be very effective in protecting the cells against radiation‐induced suppression of glutathione peroxidase, catalase and superoxide dismutase (SOD) activities. Both curcumin and the complex protected radiation‐induced protein carbonylation and lipid peroxidation in lymphocytes with the complex showing better protection than curcumin. It also showed better overall protection by decreasing the radiation‐induced apoptosis. The kinetics of activation of PKCδ and NFκB after irradiation in presence or absence of these compounds was looked at to identify the molecular mechanism involved. The modulation of irradiation‐induced activation of PKCδ and NFκB by curcumin and the complex was found different at later time periods although the initial response was similar. The early responses could be mere stress responses and the activation of crucial signaling factors at later time periods may be the determinants of the fate of the cell. In this study this delayed effect was observed in case of complex but not in case of curcumin. The delayed effect of the complex along with the fact that it is a better free radical scavenger must be the reason for its better efficacy. The complex was also found to be less cytotoxic then curcumin at similar concentration. J. Cell. Biochem. 102: 1214–1224, 2007. © 2007 Wiley‐Liss, Inc.
Acetylation of histone H4 in complex structural transitions of spermiogenic chromatin
Authors: Kurtz Kathryn , Martínez‐Soler Fina , Ausió Juan , Chiva Manel 
Published: April 2007

AbstractIn spermiogenic nuclei of the cephalopod mollusc Sepia officinalis histones are replaced by a precursor‐protamine molecule, which is later converted into protamine. Simultaneously, spermiogenic chromatin undergoes a complex structural change. Somatic‐like chromatin belonging to the earliest spermatid is progressively reorganized into: (a) granules of 20 nm diameter, (b) fibres of 30–35 nm, and (c) fibres of 40–50 nm. In the final phases of spermiogenesis these fibres of 40–50 nm join to form larger structures of condensed chromatin, and lastly, the uniformly packed chromatin in the sperm nucleus. Using specific antibodies for mono‐ and hyperacetylated forms of histone H4, in this work we show that the first structural remodelling of chromatin (from somatic‐like organization into 20 nm granules) is given concomitantly with a massive mono‐acetylation of H4 (acetylation in lysine 12), whereas the structural remodelling from 30–35 to 40–50 nm fibres is produced simultaneously with hyperacetylation of H4 and the nuclear removal of histones. J. Cell. Biochem. 102: 1432–1441, 2007. © 2007 Wiley‐Liss, Inc.
Transcriptional regulation of IL‐8 by iron chelator in human epithelial cells is independent from NF‐κB but involves ERK1/2‐ and p38 kinase‐dependent activation of AP‐1
Authors: Choi Eun‐Young , Park Zee‐Yong , Choi Eun‐Ju , Oh Hyun‐Mee , Lee SungGa , Choi Suck‐Chei , Lee Kang‐Min , Im Sin‐Hyeog , Chun Jang‐Soo , Jun Chang‐Duk 
Published: April 2007

AbstractWe have shown that the bacterial iron chelator, deferoxamine (DFO), triggers inflammatory signals including the production of CXC chemokine IL‐8, in human intestinal epithelial cells (IECs) by activating the ERK1/2 and p38 kinase pathways. In this study we investigated the mechanisms involved in IL‐8 generation by DFO, focusing on the transcription factors involved and the roles of both mitogen‐activated protein kinases (MAPKs) in the transcription factor activation. Treatment of human epithelial HT‐29 cells with DFO markedly up‐regulated the expression of the essential components of the transcription factor AP‐1 at a transcriptional level, while it minimally affected the expression of the NF‐κB subunits. DFO also induced AP‐1‐dependent transcriptional activity in HT‐29 cells, and this activity was further augmented by the wild‐type c‐Jun transfection. In contrast, the AP‐1 activity by DFO was markedly decreased by the dominant‐negative c‐Jun transfection. Electrophoretic mobility shift assays revealed that DFO increases the specific binding of AP‐1 but not of NF‐κB. Such AP‐1 binding and transcriptional activities were blocked by the inhibitors of the ERK1/2 and p38 kinase pathways, suggesting that both mitogen‐activated protein kinases (MAPKs) lie upstream of AP‐1. Besides its action on AP‐1, DFO also induced the specific binding of other transcription factors such as CREB and Egr‐1. In summary, our results indicate that iron chelator‐induced IL‐8 generation in IECs involves activation of ERK1/2 and p38 kinase and downstream activation of AP‐1. A possible link between iron status and two additional transcription factors, that is, CREB and Egr‐1, rather than NF‐κB, was also suggested. J. Cell. Biochem. 102: 1442–1457, 2007. © 2007 Wiley‐Liss, Inc.
Two inducible, functional cyclooxygenase‐2 genes are present in the rainbow trout genome
Authors: Ishikawa Tomo‐o , Herschman Harvey R. 
Published: April 2007

AbstractThe cyclooxygenases (Cox) catalyze the initial reactions in prostanoid biosynthesis, and produce the common prostanoids precursor, PGH2. Mammalian species have two Cox isoforms; constitutively expressed cyclooxygenase‐1 (Cox‐1) and inducible cyclooxygenase‐2 (Cox‐2). Database searches suggest three Cox genes are present in many fish species. In this study, we cloned and characterized a second Cox‐2 cDNA, Cox‐2b, from the rainbow trout. Rainbow trout Cox‐2b protein contains all the functionally important conserved amino acids for Cox enzyme activity. Moreover, the Cox‐2b message contains AU‐rich elements (AREs) in the 3′ untranslated region (3′UTR) characteristic of inducible Cox‐2 mRNAs. We took advantage of the existence of a rainbow trout cell line to demonstrate that expression from both the originally reported Cox‐2 (Cox‐2a) and Cox‐2b genes is inducible. However, differential induction responses to alternative inducers are observed for rainbow trout Cox‐2a and Cox‐2b. Both Cox‐2a and Cox‐2b proteins expressed in COS cells are enzymatically active. Thus the rainbow trout has two functional, inducible Cox‐2 genes. The zebrafish also contains two Cox‐2 genes. However, genome structure analysis suggests diversion of the Cox‐2a gene between zebrafish and rainbow trout. J. Cell. Biochem. 102: 1486–1492, 2007. © 2007 Wiley‐Liss, Inc.
Cellular and organismal ageing: Role of the p53 tumor suppressor protein in the induction of transient and terminal senescence
Authors: Schmid Gerald , Kramer Matthias P. , Maurer Margarita , Wandl Stefanie , Węsierska‐Gądek Józefa 
Published: April 2007

AbstractIn recent years, an impact of the p53 tumor suppressor protein in the processes of cellular and organismal ageing became evident. First hints were found in model organisms like Saccharomyces cerevisiae, Caenorhabditis elegans, and Drosophila melanogaster where a clear connection between ageing phenotypes and pathways that are regulated by p53, were found. Interestingly, pathways that are central to the ageing process are usually also involved in energy metabolism and are highly conserved throughout evolution. This also supports the long known empiric finding that caloric restriction has a positive impact on the life span of a wide variety of organisms. Within the last years, on the molecular level, an involvement of the insulin‐like growth factor and of the histone deacetylase SRIT1 could be shown. Insight on the impact of p53 on ageing at the organismal level came from mice expressing aberrant forms of the p53 protein. Obviously, the balance of the full length p53 protein and of the shorter p44/ΔNp53 isomer bear a strong impact on ageing. The shorter isoform regulates full length p53 and in cases where there is too much of the longer isoform, this leads to elevated apoptosis resulting in decreased tumor incidence but also in premature ageing due to exhaustion of the renewal potential. Therefore, modulating the expression of the truncated p53 isoform accordingly, might lead to increased health‐span and elevated life‐span. J. Cell. Biochem. 101: 1355–1369, 2007. © 2007 Wiley‐Liss, Inc.
Theoretical study on the low‐lying electronic states of NiH and NiAt
Authors: Zou Wenli , Liu Wenjian 
Published: April 2007

AbstractThe low‐lying electronic states of NiH and NiAt are investigated by using multireference second‐order perturbation theory with relativistic effects taken into account. The potential energy curves as well as the corresponding spectroscopic constants are reported. The results are grossly in good agreement with the available experimental data and should thus be very useful for guiding future experimental measurements. A cross comparison with other nickel monohalides NiX (X = F, Cl, Br, and I) reveals that the change in the spin–orbit splittings when going from lighter to heavier ligands results more from the state interaction than from the relativistic effects of the ligands. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
Nonsurgical management of thyroid abscess with sonographically guided fine needle aspiration
Authors: Ilyin Alexey , Zhelonkina Natalia , Severskaya Natalia , Romanko Svetlana 
Published: April 2007

AbstractTreatment of thyroid abscess commonly includes the surgical drainage along with systemic antibiotic therapy. Alternatives for open surgical intervention may be the conservative management with use needle aspiration or catheter drainage. We report here two cases of thyroid abscess treatment with 21‐gauge needle aspiration under ultrasound guidance. In each case needle drainage was performed twice, at the 1st and 5th day of admission. Antibiotics were administered in pills and injected into the abscess cavity followed the pus aspiration and lavage. Both patients were cured. Follow‐up has not revealed recurrence during 6 month and 5 years. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2007
Iatrogenic venous pseudoaneurysm following venipuncture: Case report and review of the literature
Authors: Lotfi Mehrzad , Nabavizadeh Seyed Ali , Abbasi Hamid Reza , Geramizadeh Bita 
Published: April 2007

AbstractWe report the case of a venous pseudoaneurysm that developed after a venipuncture made during a blood donation in an otherwise healthy 43‐year‐old man. The patient presented 3 years later with a mass in the antecubital fossa that was successfully treated with surgical resection. The clinical features of this exceptionally rare lesion are discussed. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2007
Lower blood flow velocity, higher resistance index, and larger diameter of extracranial carotid arteries are associated with ischemic stroke independently of carotid atherosclerosis and cardiovascular risk factors
Authors: Bai Chyi‐Huey , Chen Jiunn‐Rong , Chiu Hou‐Chang , Pan Wen‐Harn 
Published: April 2007

AbstractPurpose.To investigate the association between diameter and flow velocity of the carotid arteries and ischemic stroke.Methods.Peak systolic velocity, end diastolic velocity, Pourcelot resistance index, blood flow volume, luminal diameter, and carotid plaque burden were measured and compared in 240 ischemic stroke (IS) patients without history of stroke, 163 chronic stable IS patients, and 236 nonstroke controls (age, ≥40 years). Data were also compared between stroke subtypes (large artery atherosclerosis, lacunar, cardioembolic, or undetermined origin).Results.Acute as well as chronic stable IS patients had significantly lower flow velocities and flow volume, higher resistance index than nonstroke controls in the common carotid artery (CCA), internal carotid artery and external carotid artery, and larger common carotid artery diameter. The differences were found across all IS subtypes and in stroke patients with as well as without carotid plaque. Comparisons between these subgroups showed significant differences in end diastolic velocity, resistance index, flow velocity, and diameter that were more prominent in the CCA. After adjusting for carotid plaque and cardiovascular risk factors, the associations between the above‐mentioned parameter and stroke remained significant.Conclusions.Stroke patients in acute as well as chronic stable phase appeared to have larger CCA diameters, lower carotid flow velocities and volume, and higher resistance index than nonstroke patients independently of extracranial carotid atherosclerosis. These findings need to be confirmed by a prospective study. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2007
Partial demineralization of maple sap by electrodialysis: impact on syrup sensory and physicochemical characteristics
Authors: Bazinet Laurent , Gaudreau Hélène , Lavigne Darie , Martin Nathalie 
Published: April 2007

AbstractIn this project, samples of osmosed maple saps were demineralized to 12.5 and 25% levels by electrodialysis (ED). The effect of this treatment on the composition and the physicochemical and sensory properties of maple syrups obtained from demineralized maple sap was evaluated. The ED technology was efficient to decrease levels of malic acid and calcium in osmosed maple saps. Effectively, 38% and 24% decreases in malic acid and calcium respectively were reached for ED with a demineralization level of 25% without any changes in the other measured components of osmosed maple saps. The demineralization process had no effect on the yield of syrups produced and on their characteristics: no significant difference was observed during sensory analysis and viscosity. Moreover, the percentage of light transmission of syrups produced from demineralized osmosed saps was higher than for the control. This work suggests that ED could be a potential technology to decrease or avoid sugar sand formation during maple syrup production. Copyright © 2007 Society of Chemical Industry
The ripening and aging of noni fruits (Morinda citrifolia L.): microbiological flora and antioxidant compounds
Authors: Chan‐Blanco Yanine , Vaillant Fabrice , Pérez Ana M , Belleville Marie‐Pierre , Zúñiga Cira , Brat Pierre 
Published: April 2007

AbstractThe juice of noni fruit (Morinda citrifolia L.) is claimed to be a natural functional beverage with a growing market both in the USA and Europe. It is traditionally produced by keeping harvested fruit in closed containers for several weeks as the fruit senesces or ages. Little is known about the changes that occur in the juice's microbiological, physicochemical, and functional characteristics during this treatment. Traditional processing was simulated in the laboratory, with samples being recovered and analyzed at various time intervals. At first, fermentation occurred and populations of molds, yeasts, and mesophilic bacteria increased significantly. After 2 weeks, microbial growth changed abruptly, stopping for yeasts, molds, and mesophilic bacteria, and decreasing suddenly for lactic bacteria. Analyses of pH, soluble solids, ethanol, and lactic acid in the fruits confirmed the microbial analyses, indicating initial sensitive variations, followed by values remaining comparatively steady during aging. Vitamin C and total phenol contents also remained constant at 300 ± 60 mg and 50 ± 20 mg GAE, respectively, per 100 g of pulp. Antioxidant capacity likewise remained relatively high at 8 ± 1.5 µmol Trolox® g−1. All phenolic compounds, including scopoletin and rutin, varied significantly immediately after harvest but remained more or less steady during aging. Copyright © 2007 Society of Chemical Industry
The potential use of hydrolysed konjac glucomannan as a prebiotic
Authors: Al‐Ghazzewi Farage Hashmi , Khanna Sheila , Tester Richard Frank , Piggott John 
Published: April 2007

AbstractKonjac glucomannan hydrolysate was derived enzymatically from konjac flour under optimal conditions. A number of culture strains of lactobacilli and bifidobacteria were grown on De Man, Rogosa and Sharpe (MRS) media supplemented with the hydrolysate. This hydrolysate stimulated the growth of all strains examined. Colony sizes of those strains grown on konjac hydrolysate were significantly (P = 0.001) bigger than those grown on pectin or xylan hydrolysates. Bacterial growth profiles were also conducted on nutrient agar (MRS or modified MRS agar containing konjac hydrolysate) using single strains of lactobacilli or bifidobacteria (Lactobacillus acidophilus, Lactobacillus casei or Bifidobacterium adolescentis), single pathogen cultures (Escherichia coli or Listeria monocytogenes) or mixed bacterial cultures (from chicken breast extract). Although the growth of lactobacilli inhibited the growth of pathogens (single or mixed culture) the pathogens could not grow on the konjac hydrolysate as a sole carbon source. Microbial growth profiles using konjac hydrolysate or inulin in UHT milk were also investigated. The results showed that the numbers of colony forming units (cfu) obtained from milk containing the konjac hydrolysate were significantly (P = 0.01) higher than those containing inulin. It is suggested that the unique properties of konjac hydrolysate make it universally valuable as a prebiotic which can be applied to a wide range of foods, feeds and healthcare/pharmaceutical products. Copyright © 2007 Society of Chemical Industry
Enhancing the productivity of high‐magnesium soil and water resources in Central Asia through the application of phosphogypsum
Authors: Vyshpolsky F. , Qadir M. , Karimov A. , Mukhamedjanov K. , Bekbaev U. , Paroda R. , Aw‐Hassan A. , Karajeh F. 
Published: April 2007

AbstractRecent evidences from some irrigated areas worldwide, such as Central Asia, suggest that water used for irrigation contains magnesium (Mg2+) at levels higher than calcium (Ca2+). Excess levels of Mg2+ in irrigation water and/or in soil, in combination with sodium (Na+) or alone, result in soil degradation because of Mg2+ effects on the soil's physical properties. More than 30 per cent of irrigated lands in Southern Kazakhstan having excess levels of Mg2+ are characterized by low infiltration rates and hydraulic conductivities. The consequence has been a gradual decline in the yield of cotton (Gossypium hirsutum L.), which is commonly grown in the region. These soils require adequate quantities of Ca2+ to mitigate the effects of excess Mg2+. As a source of Ca2+, phosphogypsum—a byproduct of the phosphorous fertilizer industry—is available in some parts of Central Asia. In participation with the local farming community, we carried out a 4‐year field experiment in Southern Kazakhstan to evaluate the effects of soil application of phosphogypsum—0, 4·5, and 8·0 metric ton per hectare (t ha−1)—on chemical changes in a soil containing excess levels of Mg2+, and on cotton yield and economics. The canal water had Mg2+ to Ca2+ ratio ranging from 1·30 to 1·66 during irrigation period. The application of phosphogypsum increased Ca2+ concentration in the soil and triggered the replacement of excess Mg2+ from the cation exchange complex. After harvesting the first crop, there was 18 per cent decrease in exchangeable magnesium percentage (EMP) of the surface 0·2 m soil over the pre‐experiment EMP level in the plots where phosphogypsum was applied at 4·5 t ha−1, and a 31 per cent decrease in EMP in plots treated with phosphogypsum at 8 t ha−1. Additional beneficial effect of the amendment was an increase in the soil phosphorus content. The 4‐year average cotton yields were 2·6 t ha−1 with 8 t ha−1 phosphogypsum, 2·4 t ha−1 with 4·5 t ha−1 phosphogypsum, and 1·4 t ha−1 with the control. Since the amendment was applied once at the beginning, exchangeable Mg2+ levels tended to increase 4 years after its application, particularly in the treatment with 4·5 t ha−1 phosphogypsum. Thus, there would be a need for phosphogypsum application to such soils after every 4–5 years to optimize the ionic balance and sustain higher levels of cotton production. The economic benefits from the phosphogypsum treatments were almost twice those from the control. Copyright © 2007 John Wiley & Sons, Ltd.
A Note on Classes of BANACH Spaces Related to Stable Measures
Authors: Mathé Peter 
Published: April 2007

AbstractThe problem under consideration is the following: Let S: E′ → Lq, T: E′ → Lp, 0 < q ≦ 2, 0 < p ≦ 2, be operators, ‖Sa‖ ≦ ‖Ta‖, such that, T generates a stable measure on E, i.e., exp (‐‖Ta‖ p), a ϵ E′, is the characteristic function of a RADON measure on E. Does this imply, that exp (‐‖Sa‖ q), a ϵ E′, is the characteristic function of a RADON measure, too? In general this is not true provided q or p less than 2. A BANACH space is said to be of (q,p)‐cotype if the answer to the above question is “yes”. We establish several properties of this classification and obtain as an application the well‐known classes due to MOUCHTARI, TIEN, WERON and MANDREKAR, WERON, Finally we apply our results to so‐called S‐spaces.