bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cdk9/Cyclin T1 complex: A key player during the activation/differentiation process of normal lymphoid B cells
Authors: De Falco Giulia , Leucci Eleonora , Onnis Anna , Bellan Cristiana , Tigli Chiara , Wirths Stefan , Cerino Giovanna , Cocco Mario , Crupi Domenica , De Luca Antonio , Lanzavecchia Antonio , Tosi Piero , Leoncini Lorenzo , Giordano Antonio 
Published: January 2008

AbstractCdk9/Cyclin T1 complex is very important in controlling specific differentiative pathways of several cell types. Limited data are available regarding the expression of Cdk9/Cyclin T1 in hematopoietic and lymphoid tissues. Cdk9/Cyclin T1 expression seems to be related to particular stages of lymphoid differentiation/activation. In this study, we observed that the expression level of Cdk9/Cyclin T1 in vivo increases in memory B cells compared to naïve B cells, and in activated B cells, compared to non‐activated ones. The expression level of the Cdk9/Cyclin T1 complex does not increase in cells induced to differentiate in vitro. In addition, we showed that Cdk9 interacts with E12 and E47, specifically activated during Germinal Center (GC) reaction. Taken together this data suggests an active role for the Cdk9/Cyclin T1 complex during lymphoid differentiation through germinal center reaction. J. Cell. Physiol. 215: 276–282, 2008. © 2008 Wiley‐Liss, Inc.
Comparison of synthesis yields of 3′‐deoxy‐3′‐[18F]fluorothymidine by nucleophilic fluorination in various alcohol solvents
Authors: Lee Sang Ju , Oh Seung Jun , Chi Dae Yoon , Lee Byoung Se , Ryu Jin Sook , Moon Dae Hyuk 
Published: January 2008

Abstract[18F]Fluorothymidine ([18F]FLT) is synthesized with a high radiochemical yield by nucleophilic substitution in protic solvent. In this study, we compared [18F]fluorination yields of [18F]fluorothymidine ([18F]FLT) in various alcohol solvents: 3,3‐dimethyl‐1‐butanol, 2‐trifluoromethyl‐2‐propanol, t‐BuOH (2‐methyl‐2‐propanol), t‐amyl alcohol (2‐methyl‐2‐butanol), thexyl alcohol (2,3‐dimethyl‐2‐butanol) and 3,3‐dimethyl‐2‐butanol. We used 5′‐O‐DMTr‐2′‐deoxy‐3′‐O‐nosyl‐β‐D‐threopentofuranosyl)‐3‐N‐BOC‐thymine as a precursor for [18F]fluorination. [18F]F− was eluted with TBAHCO3 solution after trapping [18F]F− on a PS‐HCO3 cartridge. [18F]fluorination was performed at 100°C for 5–30 min using 20 mg of the precursor. [18F]fluorination and radiochemical yields of [18F]FLT were evaluated by radioTLC. [18F]fluorination yields were dependent on the solvent used. All tertiary alcohol solvents, except 2‐trifluoromethyl‐2‐propanol, showed >85% of [18F]fluorination yields, whereas primary and secondary alcohols showed 26.3–71.8%. The highest yield of 94.1±4.4% was obtained with thexyl alcohol after [18F]fluorination for 5 min. Automated synthesis with t‐amyl alcohol resulted in high synthetic yields of 64.6±6.1% after high‐performance liquid chromatography purification (n=43). The use of tertiary alcohol as a solvent provides high radiochemical yields of [18F]FLT. Copyright © 2008 John Wiley & Sons, Ltd.
Characterization of normal canine anterior cruciate ligament–associated synoviocytes
Authors: Vasanjee Sunil C. , Paulsen Daniel , Hosgood Giselle , Robinson Sandra O. , Lopez Mandi J. 
Published: January 2008

AbstractThis study was designed to identify and quantify synoviocyte phenotypes enveloping the canine anterior cruciate ligament (ACL) to test the hypothesis that there are at least two synoviocyte phenotypes, each with distinct quantities and topographical distributions. CD18 and HSP25 epitopes were colocalized in the synovium of 10 normal canine ACLs. Sagittal sections were prepared from medial, central, and lateral aspects of each ACL and phenotypes were quantified in the proximal, middle, and distal aspects of each section. Distinct synoviocyte populations stained positive for CD18 (CD18+) or HSP25 (HSP25+), and a small population of cells stained for both epitopes (DS+). The proportion (mean ± SEM) of HSP25+ synoviocytes (57% ± 7.5%) was significantly greater than the proportion of CD18+ synoviocytes (27% ± 8.2%), which was significantly greater than the proportion of DS+ synoviocytes (16% ± 3.5%). Reverse transcriptase polymerase chain reaction (RT‐PCR), Western blot analysis, and immunoelectron microscopy confirmed the presence of CD18 and HSP25 epitopes in the canine ACL. Identification and quantification of ACL synoviocytes may serve as the foundation for future studies involving ACL disease or reconstruction. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:809–815, 2008
Raman spectroscopic analysis of azurite blackening
Authors: Mattei E. , de Vivo G. , De Santis A. , Gaetani C. , Pelosi C. , Santamaria U. 
Published: January 2008

AbstractAzurite is a basic copper carbonate pigment largely employed in painting realization. The areas painted with azurite are easily alterable and are often less resistant than the other parts of artworks. The azurite alteration in a black pigment, the copper oxide (tenorite), has been studied by micro‐Raman spectroscopy. The blackening can be due to thermal or chemical alterations: in the second case the alterations being due to the presence of alkaline conditions. Laser‐induced degradation of azurite has been studied as a function of the grain size. The results show that the temperature of the grains decreases as the size increases, and azurite degrades into tenorite only below the critical value of 25 µm. To study the chemical alteration of azurite, the pigment has been applied on the plaster of terracotta samples and analyzed at different pH values by micro‐Raman spectroscopy. As opposed to most part of the analytical techniques, it can detect the presence of both azurite and tenorite molecules in the same micro areas, and provides a valuable tool to determine azurite degradation. Copyright © 2008 John Wiley & Sons, Ltd.
Raman spectroscopy of ion‐irradiated astrophysically relevant materials
Authors: Baratta G. A. , Brunetto R. , Leto G. , Palumbo M. E. , Spinella F. , Strazzulla G. 
Published: January 2008

AbstractSolid objects in space (interstellar grains, comets, interplanetary dust particles, etc.) are continuously exposed to energetic processes, such as cosmic ion irradiation, that influence their evolution. In this paper we present an experimental study, carried out by Raman spectroscopy, of the effects induced by ion irradiation on frozen ices and refractory materials. If the irradiated ice mixture contains a relevant amount of carbon atoms, the ice is converted into an organic residue (stable at room temperature), which at high irradiation dose evolves toward a hydrogenated amorphous carbon. Here we show that material similar to that produced in the laboratory by ion irradiation of frozen ice mixtures and refractory materials can be formed in space by cosmic ion irradiation. This finding has been recently confirmed by the Stardust mission, which revealed in some of the cometary particles collected in space and returned to earth carbonaceous materials that have been processed by cosmic ion irradiation. Copyright © 2008 John Wiley & Sons, Ltd.
Second‐order Raman scattering from linear carbon chains inside multiwalled carbon nanotubes
Authors: Cupolillo A. , Castriota M. , Cazzanelli E. , Caputi L. , Giallombardo C. , Mariotto G. , Papagno L. 
Published: January 2008

AbstractThe present investigation concerns multiwalled carbon nanotubes synthesized on graphite cathodes by arc discharge in a He atmosphere, either with the insertion of a catalytic NiCr mixture or without catalysts. The morphology of the deposited cathodes was investigated by SEM, while the amount of carbon chains inside multiwalled carbon nanotubes (C@MWCNT) in various regions of the deposited cathodes was revealed by a parallel micro‐Raman study, through the analysis of the signal from the Raman bands generated by C@MWCNT in the range 1780–1870 cm−1 (L bands). In samples obtained by using the catalyst, a very high concentration of linear carbon chains is found in some zones, as indicated by the intensity of the L band, which turns out remarkably stronger than the G band of the host nanotubes. In these zones, the second‐order Raman scattering is clearly observed, too. The experimental wavenumber values of the 2 L overtone are slightly lower than the exact doubling of the one‐phonon peak wavenumber, and this fact is discussed in terms of the existing theoretical predictions for the chain‐mode dispersion curve. Copyright © 2008 John Wiley & Sons, Ltd.
Raman spectroscopy of Bi‐Te thin films
Authors: Russo V. , Bailini A. , Zamboni M. , Passoni M. , Conti C. , Casari C. S. , Li Bassi A. , Bottani C. E. 
Published: January 2008

AbstractThe deposition of micro‐ and nanocrystalline bismuth telluride thin films with tailored structure and composition is of interest in view of improving the well‐known material thermoelectric properties. Only a few works exist that discuss Raman scattering of Bi2Te3 crystals and films, while a Raman characterization of other phases, i.e. other lesser known compounds of the Bi‐Te system, such as tsumoite (BiTe) and pilsenite (Bi4Te3), is still completely lacking. We here present a Raman investigation of Bi‐Te polycrystalline thin films with controlled structure (stoichiometry and growth orientation), morphology and phase composition, produced by nanosecond pulsed laser deposition. Interpretation of Raman spectra from Bi‐Te films was supported by scanning electron microscopy, energy dispersive spectroscopy (EDS) and X‐Ray diffraction measurements, together with the predictions of the group theory. In this way, the first Raman characterization of Bi‐rich phases (namely BiTe and Bi4Te3) has been obtained. For Bi‐Te compositions characterized by a high Bi or Te content, Raman spectra reveal that segregation of elemental Bi or Te occurs. Copyright © 2008 John Wiley & Sons, Ltd.
Micro‐Raman investigations on the fresco ‘Trapasso della Vergine’ in the Church of ‘S. Giovanni Battista’ of Paterno Calabro in southern Italy
Authors: Castriota Marco , Meduri Emanuela , Barone Tiziana , De Santo Giuseppe , Cazzanelli Enzo 
Published: January 2008

AbstractIn this work, a micro‐Raman spectroscopic investigation was carried out to identify the pigments used in the fresco ‘Trapasso della Vergine’. This fresco is in the Church of S. Giovanni Battista (St. John the Baptist) in Paterno Calabro, southern Italy, and is believed to have been painted during the first half of the 16th century, on the basis of the available historical documents. The analysis was performed on five specimens belonging to significant zones of the fresco, detached because of the degradation processes. Raman spectra were collected by a Jobin Yvon micro‐Raman LABRAM apparatus, with a HeNe laser (632.8 nm) as excitation source. The white, blue and red pigments were identified. Some indications were obtained about the geographical origin of the pigments and a restoration in the 19th century. Copyright © 2008 John Wiley & Sons, Ltd.
Theoretical Designs of Molecular Photonics Materials
Authors: Yi Yuanping , Zhu Lingyun , Shuai Zhigang 
Published: January 2008

AbstractDifferent theoretical models for the computation of multiphoton absorption response are described and reviewed. The influences of intrinsic molecular structure parameters on the multiphoton absorption properties for organic conjugated molecules are discussed in terms of: i) the donor/acceptor substitutions; ii) the π conjugation length; iii) the ground‐state polarization mimicking structural character such as bond‐length alternation; iv) the molecular dimensionality; and, v) some other possible factors, such as vibronic coupling and the solvent, as well as aggregation effects. Some theoretical designing strategies for organic materials with large multiphoton cross‐sections are then proposed.magnified image
Thermodynamic hydrogen bonding inpoly(styrene‐co‐acrylic acid)/poly(styrene‐co‐4‐N,N‐dimethylacrylamide) blends
Authors: ElMiloudi K. , Hadj Hamou A.S. , Djadoun S. 
Published: January 2008

AbstractFTIR study of the hydrogen bonding interactions within blends of different ratios of poly(styrene‐co‐acrylic acid) containing 18, 27, and 32 mol% of acrylic acid (SAA) and poly(styrene‐co‐N,N‐dimethylacrylamide) containing 17 mol% of N,N‐dimethylacrylamide (SAD‐17) was carried out qualitatively and quantitatively in the temperature range varying from room temperature to 210°C. Two new bands characterizing these interactions appeared in the 1800–1550 cm–1 region at 1730 cm–1 and 1616 cm–1 and are attributed to “liberated” carbonyl group of the acidic copolymer and the “associated amide” carbonyl group, respectively. Equilibrium constants describing both the self‐association K2 and inter‐association KA and the enthalpy of hydrogen bonding formation in the different blends were experimentally determined using a curve fitting analysis of the infra‐red spectra as a function of temperature using the appropriate equations derived from the Painter‐Coleman association model. The obtained results confirm the miscibility of these blends in the considered temperature range from the negative values of the total free energy of mixing ΔGM. Optimization of the extent of intermolecular interactions between the two polymers in these blends is investigated. POLYM. ENG. SCI., 2008. © 2008 Society of Plastics Engineers
Reversible sol–gel transitions in aqueous solutions of N‐isopropylacrylamide ionic copolymers
Authors: Krzyminski Karol J , Jasionowski Marek , Gutowska Anna 
Published: January 2008

AbstractIonic copolymers of N‐isopropylacrylamide (NIPA) exhibiting sol–gel transitions in aqueous solutions were investigated. The studies were aimed at understanding of the structure–property relationship in the design of injectable, in situ forming gels for potential biomedical applications in delivery of therapeutics and tissue engineering. Aqueous solutions of NIPA ionic copolymers were found to flow freely at ambient temperatures and formed soft gels with controlled syneresis above 32 °C, the lower critical solution temperature of NIPA. The sol–gel transitions and temperature‐dependent properties of the resulting gels were analyzed using dynamic rheometry and ultraviolet and infrared spectrometry, and were found to be controlled by the molecular weight and composition of copolymers, ionization state of comonomers and composition of aqueous solvent. Copyright © 2008 Society of Chemical Industry
Proteome analysis of differentiating human myoblasts by dialysis‐assisted two‐dimensional gel electrophoresis (DAGE)
Authors: Gonnet Florence , Bouazza Belaid , Millot Gaël Armel , Ziaei Simin , Garcia Luis , Butler‐Browne Gillian S. , Mouly Vincent , Tortajada Jeanine , Danos Olivier , Svinartchouk Fédor 
Published: January 2008

AbstractIn the present study, modifications in cytosolic expressed proteins during human myoblast differentiation were studied by dialysis‐assisted 2‐DE (DAGE, [1]). About 1000 spots were analysed on the 5th and 13th day of differentiation with a dynamic range of protein expression exceeding 1000‐fold. During myogenic differentiation, the number of nonmatching spots as well as the extent of quantitative differences between matched spots significantly increased. Over one hundred differentially expressed spots were excised and identified by MALDI‐TOF MS. The differentiation‐associated expression pattern of eight proteins was validated by Western blot analysis. Differential expression of several proteins was demonstrated for the first time in human myotubes. Interestingly, Ingenuity pathway analysis grouped 30 of these proteins into two overlapping networks containing as principal nodes IGF‐1 and tumour necrosis factor, two proteins known to play a crucial role in cytogenesis. Our results illustrate the large rearrangement of the proteome during the differentiation of human myoblasts and provide evidence for new partners involved in this complex process.
Glycoproteomic characterization of butyrylcholinesterase from human plasma
Authors: Kolarich Daniel , Weber Alfred , Pabst Martin , Stadlmann Johannes , Teschner Wolfgang , Ehrlich Hartmut , Schwarz Hans‐Peter , Altmann Friedrich 
Published: January 2008

AbstractHuman butyrylcholinesterase (hBChE) is a highly glycosylated protein present in human plasma. The enzyme hydrolyses choline esters, for example benzoylcholine, butyrylthiocholine and acetylthiocholine as well as noncholine esters like heroin and aspirin. hBChE is primarily involved in neuronal transmission and is a potential bioscavenger of toxic organophosphates to protect acetylcholinesterase. A prerequisite for the therapeutic use of hBChE is a detailed characterization of this glycoprotein purified from human plasma. In this study, MS/MS could confirm most of the protein backbone, including the N‐ and the C‐terminus. Site‐specific analysis of all nine potential N‐glycosylation sites revealed mainly mono‐ and disialylated N‐glycans to be present on this glycoprotein. Sialic acids (Neu5Ac) are mainly α2,6‐linked, however a fraction of the N‐glycans contained Neu5Ac also in α2,3 linkage. On monosialylated N‐glycans, sialic acid is exclusively located on the 3‐arm and in α2,6 linkage, as verified by 2D‐HPLC and exoglycosidase digests of 2‐aminopyridine (PA)‐labelled N‐glycans. This first comprehensive glycoproteomic analysis of the important human plasma glycoprotein BChE did not give any indication of O‐glycosylation or any other kind of PTMs as previously postulated.
Exploring snake venom proteomes: multifaceted analyses for complex toxin mixtures
Authors: Fox Jay W. , Serrano Solange M. T. 
Published: January 2008

AbstractSnake venom proteomes are complex mixtures of a large number of distinct proteins. In a sense, the field of snake venom proteomics has been under investigation since the very earliest biochemical studies on venoms where peptides and proteins were isolated and structurally and biologically characterized. With the recent developments in mass spectrometry for the identification of proteins, coupled with venom gland transcriptomes, has the field of snake venom proteomics began to flourish. These developments have led to exciting insights into the protein composition of venoms and subsequently their pathological activities. In this review, we will discuss the state of art of snake venom proteomics. Although we have not reached the ultimate goal of characterizing and quantifying all unique proteins in a venom proteome, current technologies have opened many opportunities for high‐throughput proteomic studies that have gone beyond simple protein identification to analyzing various functional aspects, such as post‐translational modifications, proteolytic processing and toxin‐target interactions. In this review, we will discuss the technological approaches used in the study of venom proteomics highlighting the advances made and future directions.
Sociomics: CESAGen Multidisciplinary Workshop on the Transformation of Knowledge Production in the Biosciences, and its Consequences 23–24 July, 2007 Wellcome Trust Genome Campus, Hinxton, UK
Authors: McNally Ruth 
Published: January 2008

AbstractOn 23 and 24 July, 2007, the Centre for Economic and Social Aspects of Genomics (CESAGen) held its first sociomics workshop at the Wellcome Trust Genome Campus, Hinxton, UK. The topic was transformation of knowledge production. Participants included social scientists together with those working on different elements of the proteomics knowledge production‐line, including core facilities, data repositories, large‐scale projects, MS, search engines, reference databases, standardisation and public funding. Recurrent motifs included gear‐heads, black boxes, uncertainty and getting back to biology.
BSPR/EBI 2007 meeting report – Integrative Proteomics: From Molecules to Systems July 25–27, 2007 Wellcome Trust Conference Centre, Hinxton, UK
Authors: Donoghue Pamela , Byrne Jennifer , Pennington Kyla 
Published: January 2008

AbstractThis report reviews the joint British Society for Proteome Research (BSPR) and European Bioinformatics Institute (EBI) 2007 meeting, ‘Integrative Proteomics: From Molecules to Systems’ which took place at the Wellcome Trust Conference Centre, Hinxton, UK, from 25th to 27th July. The aim of this year's meeting was to explore how the integration of ‘omic’ technologies can lead to a comprehensive understanding of cellular organization, differentiation and signalling. Studies investigating protein–protein interactions and trafficking illustrated how the combination of proteomics and bioinformatics is allowing systems biology to develop as a discipline in its own right.
‘Proteomic Basics – Sample Preparation and Separation’: The 1st European Summer School in Kloster Neustift 12–18 August, 2007 Brixen/Bressanone, South Tyrol, Italy
Authors: Marcus Katrin , Kühn‐Hölsken Eva , Schmidt Carla , Schulenborg Thomas , Urlaub Henning 
Published: January 2008

AbstractProteomics is rapidly developing into a routine approach for protein analysis in many laboratories. The series of European‐wide Summer Schools ‘Proteomics Basics’ (http://www.proteomic‐basics.eu/) aims at teaching of comprehensive knowledge in proteomics research and applied technologies for master and graduate students and postdocs currently moving into the field of proteomic research. In the next 3 years the series will cover the theoretical basis of the fundamental topics in the various areas of proteomic analysis, i.e. sample preparation and handling, mass spectrometry, post‐translational modifications and quantitation given by leading experts in the field. This summer school series embodies a unique advantage in comparison with conventional scientific meetings and university curricula: internationally renowned experts will give a detailed perspective view of the fundamentals of their particular proteome research area, something which is usually not encountered at conferences and congresses. Here, we give a report on the first European Summer School ‘Sample Preparation and Handling’ within the series ‘Proteomic Basics’ that was held at the monastery in Neustift close to Bressanone/Brixen, Italy from August 12 to 18, 2007.
Information satisfaction in breast and prostate cancer patients: implications for quality of life
Authors: Davies Nicola J. , Kinman Gail , Thomas Robert J. , Bailey Tracey 
Published: January 2008

AbstractObjectives: This study aimed to assess information needs and levels of information satisfaction in breast and prostate cancer patients. It further examined relationships between information satisfaction and multi‐dimensional quality of life (QoL).Methods: An adapted Information Satisfaction Questionnaire (ISQ, 2004) and the Functional Assessment of Cancer Therapy QoL questionnaire (FACT‐G, 1993) were randomly distributed to cancer patients during oncology clinic visits (breast cancer, n=102; prostate cancer, n=112). Hierarchal regression analyses examined information satisfaction as a predictor of global QoL and its four dimensions (i.e. physical, social, emotional, and functional well‐being).Results: High levels of information needs and desire for decision involvement were identified, with patients expressing a considerable degree of information satisfaction. After controlling for demographic and illness factors, information satisfaction explained 21% of the variance in global QoL, 12% in physical well‐being, 13% in social well‐being, 8% in emotional well‐being, and 10% in functional well‐being (all p<0.001).Conclusions: This study highlights the importance of information satisfaction for perceived QoL in individuals with cancer. It is clearly important to identify specific information requirements during the diagnosis and treatment process in order to provide information that is congruent with patients' needs. Copyright © 2007 John Wiley & Sons, Ltd.