bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the efficacy of mesenchymal stem cells derived conditioned medium in the treatment of striae distensae: a double blind randomized clinical trial
Authors: Behrangi Elham , Feizollahi Masoomeh , Zare Sona , Goodarzi Azadeh , Ghasemi Mohammad Reza , Sadeghzadeh-Bazargan Afsaneh , Dehghani Abbas , Nouri Maryam , Zeinali Roya , Roohaninasab Masoomeh , Nilforoushzadeh Mohammad Ali 
Published: March 2024

Abstract
Background
Striae distensae is a disfiguring atrophic skin condition that impairs the body’s aesthetic image. Despite the variety of conducted studies, there is controversy regarding the best modalities. Human mesenchymal stem cells are considered a rich source for scar treatment. Skin needling is among the most efficient and safe aesthetic and therapeutic devices. This study aimed to evaluate the efficacy of the combination of needling and intradermal injection of mesenchymal stem cells compared to skin needling alone for treating striae distensae.

Method
This study was a randomized, double-blind clinical trial involving 10 women aged 18–60. Each striae lesion was divided into two parts, with one side receiving needling and intradermal injection of conditioned medium, while the other side received needling and intradermal injection of normal saline. This treatment was administered in three sessions with three-week intervals. Patients were evaluated before the first intervention and three months after the final session. Three months after the completion of the intervention, patients’ lesions were evaluated using biometric criteria, physician evaluation, and patient self-assessment.

Results
The results demonstrated a significant improvement in dermal and complete thickness and skin density in patients treated with microneedling. All skin ultrasound parameters improved significantly in patients receiving the combination of needling and conditioned medium. When comparing the two groups, significantly higher physician and patient satisfaction was observed in the combination group. However, the comparison of biometric indices improvement wasn’t significant between these groups.

Conclusion
The combination of human mesenchymal stem cells with microneedling could be considered a novel effective option for stretch marks.
Embryonic stem cells overexpressing high molecular weight FGF2 isoform enhance recovery of pre-ganglionic spinal root lesion in combination with fibrin biopolymer mediated root repair
Authors: Lima B. H. M. , Cartarozzi L. P. , Kyrylenko S. , Ferreira R. S. , Barraviera B. , Oliveira Alexandre L. R. 
Published: March 2024

Abstract
Background
Spinal ventral root avulsion results in massive motoneuron degeneration with poor prognosis and high costs. In this study, we compared different isoforms of basic fibroblast growth factor 2 (FGF2), overexpressed in stably transfected Human embryonic stem cells (hESCs), following motor root avulsion and repair with a heterologous fibrin biopolymer (HFB).

Methods
In the present work, hESCs bioengineered to overexpress 18, 23, and 31 kD isoforms of FGF2, were used in combination with reimplantation of the avulsed roots using HFB. Statistical analysis was conducted using GraphPad Prism software with one-way or two-way ANOVA, followed by Tukey’s or Dunnett’s multiple comparison tests. Significance was set at *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

Results
For the first set of experiments, rats underwent avulsion of the ventral roots with local administration of HFB and engraftment of hESCs expressing the above-mentioned FGF2 isoforms. Analysis of motoneuron survival, glial reaction, and synaptic coverage, two weeks after the lesion, indicated that therapy with hESCs overexpressing 31 kD FGF2 was the most effective. Consequently, the second set of experiments was performed with that isoform, so that ventral root avulsion was followed by direct spinal cord reimplantation. Motoneuron survival, glial reaction, synaptic coverage, and gene expression were analyzed 2 weeks post-lesion; while the functional recovery was evaluated by the walking track test and von Frey test for 12 weeks. We showed that engraftment of hESCs led to significant neuroprotection, coupled with immunomodulation, attenuation of astrogliosis, and preservation of inputs to the rescued motoneurons. Behaviorally, the 31 kD FGF2 - hESC therapy enhanced both motor and sensory recovery.

Conclusion
Transgenic hESCs were an effective delivery platform for neurotrophic factors, rescuing axotomized motoneurons and modulating glial response after proximal spinal cord root injury, while the 31 kD isoform of FGF2 showed superior regenerative properties over other isoforms in addition to the significant functional recovery.
Development and validation of a prognostic nomogram model in locally advanced NSCLC based on metabolic features of PET/CT and hematological inflammatory indicators
Authors: Wang Congjie , Fang Jian , Jiang Tingshu , Hu Shanliang , Wang Ping , Liu Xiuli , Zou Shenchun , Yang Jun 
Published: March 2024

Abstract
Background
We combined the metabolic features of 18F-FDG-PET/CT and hematological inflammatory indicators to establish a predictive model of the outcomes of patients with locally advanced non-small cell lung cancer (LA-NSCLC) receiving concurrent chemoradiotherapy.

Results
A predictive nomogram was developed based on sex, CEA, systemic immune-inflammation index (SII), mean SUV (SUVmean), and total lesion glycolysis (TLG). The nomogram presents nice discrimination that yielded an AUC of 0.76 (95% confidence interval: 0.66–0.86) to predict 1-year PFS, with a sensitivity of 63.6%, a specificity of 83.3%, a positive predictive value of 83.7%, and a negative predictive value of 62.9% in the training set. The calibration curves and DCA suggested that the nomogram had good calibration and fit, as well as promising clinical effectiveness in the training set. In addition, survival analysis indicated that patients in the low-risk group had a significantly longer mPFS than those in the high-risk group (16.8 months versus 8.4 months, P < 0.001). Those results were supported by the results in the internal and external test sets.

Conclusions
The newly constructed predictive nomogram model presented promising discrimination, calibration, and clinical applicability and can be used as an individualized prognostic tool to facilitate precision treatment in clinical practice.
Upgrading informal areas through sustainable urban development principles
Authors: Elgohary Maha Mohamed , Abdin Ahmed Reda , Khalil Heba Allah E. 
Published: March 2024

AbstractInformal areas are considered one of the biggest urban problems worldwide; it was estimated in 2018 that 29% of the world’s urban population lived in informal areas (The world bank, Population living in slums, 2022). This research aimed to identify a set of criteria and principles that are the basis for sustainable development in informal areas to achieve a better quality of life for the community. After conducting a literature review of the sustainable development principles, indices, and sustainable development interventions within informal areas, the research links them to set informal areas’ sustainable development principles. Then, by relying on the experts’ questionnaires and their analysis through SPSS, the researchers utilized the experts’ input to define each principle’s relative weight. Additionally, the research applied the extracted principles to the Boulaq El Dakrour case study to assess the sustainability of previous developments undertaken in the area with community participation.
Bridging the voice of healthcare to digital transformation in practice – a holistic approach
Authors: Frisinger Ann , Papachristou Panagiotis 
Published: March 2024

AbstractBackgroundDigital transformation is key for healthcare to meet future needs and expectations and compete equally with new actors on the market. Medical digital diagnosis tools and clinical decision support systems (CDSS) are improving and offer new opportunities. To introduce new technology in healthcare can however be a challenging mission, too often ending in failure, with excessive costs or the actual transformation work not being carried out at all. It is unclear how to drive the establishment to reach desired results in this environment, and how industrial experiences can be used to support healthcare.ObjectiveThe objective of this study was to develop a holistic approach for introducing new information technology (IT), such as a CDSS, into a primary healthcare organization supported by industry best practices for digital transformation.MethodsThis qualitative study used a combined inductive and deductive method where the perceptions and beliefs of selected primary healthcare stakeholders were used as directions for developing an approach that could utilize existing industry best practices for digital transformation.ResultsA holistic healthcare-ified approach including 20 requirements was developed, that meet the needs of healthcare. The voice of healthcare was used as prism to healthcare-ify the industry practices and adapt it to match specific healthcare conditions. An example was provided showing how the research could be put into practice.ConclusionsThis study proposed a holistic approach, based on industry best practices, but adapted to healthcare using the voice of healthcare as a bridge, that may be used to introduce CDSS and other IT into a primary healthcare organization and step up the needed digitalization.
Telomere length is an epigenetic trait – Implications for the use of telomerase-deficient organisms to model human disease
Authors: Henriques Catarina M. , Ferreira Miguel Godinho 
Published: March 2024

ABSTRACT
Telomere length, unlike most genetic traits, is epigenetic, in the sense that it is not fully coded by the genome. Telomeres vary in length and randomly assort to the progeny leaving some individuals with longer and others with shorter telomeres. Telomerase activity counteracts this by extending telomeres in the germline and during embryogenesis but sizeable variances remain in telomere length. This effect is exacerbated by the absence of fully active telomerase. Telomerase heterozygous animals (tert+/−) have reduced telomerase activity and their telomeres fail to be elongated to wild-type average length, meaning that – with every generation – they decrease. After a given number of successive generations of telomerase-insufficient crosses, telomeres become critically short and cause organismal defects that, in humans, are known as telomere biology disorders. Importantly, these defects also occur in wild-type (tert+/+) animals derived from such tert+/− incrosses. Despite these tert+/+ animals being proficient for telomerase, they have shorter than average telomere length and, although milder, develop phenotypes that are similar to those of telomerase mutants. Here, we discuss the impact of this phenomenon on human pathologies associated with telomere length, provide a brief overview of telomere biology across species and propose specific measures for working with telomerase-deficient zebrafish.
Heart rate reduction during voluntary deep diving in free-ranging loggerhead sea turtles
Authors: Saito Ayaka , Kinoshita Chihiro , Sakai Kino , Sato Katsufumi , Sakamoto Kentaro Q. 
Published: March 2024

ABSTRACT
Air-breathing vertebrates exhibit cardiovascular responses to diving including heart rate reduction (diving bradycardia). Field studies on aquatic mammals and birds have shown that the intensity of bradycardia can vary depending on diving behaviour, such as the depth of dives and dive duration. However, in aquatic reptiles, the variation in heart rate during deep dives under natural conditions has not been fully investigated. In this study, we released five loggerhead sea turtles (Caretta caretta) outfitted with recorders into the sea and recorded their electrocardiogram, depth, water temperature and longitudinal acceleration. After 3 days, the recorders automatically detached from the turtles. The heart rate signals were detected from the electrodes placed on the surface of the plastron. The mean (±s.d.) heart rate of 12.8±4.1 beats min–1 during dives was significantly lower than that of 20.9±4.1 beats min–1 during surface periods. Heart rate during dives varied with dive depth, although it remained lower than that at the surface. When the turtle dived deeper than 140 m, despite the relatively high flipper stroke rate (approximately 19 strokes min–1), the heart rate dropped rapidly to approximately 2 beats min–1 temporarily. The minimum instantaneous heart rate during dives was lower at deeper dive depths. Our results indicate that loggerhead sea turtles show variations in the intensity of diving bradycardia depending on their diving behaviour, similar to that shown by marine mammals and birds.
Adjuvant Chemoradiation in Resected Biliary Adenocarcinoma: Evaluation of SWOG S0809 with a Large National Database
Authors: Dominguez Dana A. , Wong Paul , Chen Yi-Jen , Singh Gagandeep P. , Fong Yuman , Li Daneng , Ituarte Philip H. G. , Melstrom Laleh G. 
Published: March 2024

Abstract
Background
There is a paucity of evidence supporting the use of adjuvant radiation therapy in resected biliary cancer. Supporting evidence for use comes mainly from the small SWOG S0809 trial, which demonstrated an overall median survival of 35 months. We aimed to use a large national database to evaluate the use of adjuvant chemoradiation in resected extrahepatic bile duct and gallbladder cancer.

Methods
Using the National Cancer Database, we selected patients from 2004 to 2017 with pT2-4, pN0-1, M0 extrahepatic bile duct or gallbladder adenocarcinoma with either R0 or R1 resection margins, and examined factors associated with overall survival (OS). We examined OS in a cohort of patients mimicking the SWOG S0809 protocol as a large validation cohort. Lastly, we compared patients who received chemotherapy only with patients who received adjuvant chemotherapy and radiation using entropy balancing propensity score matching.

Results
Overall, 4997 patients with gallbladder or extrahepatic bile duct adenocarcinoma with available survival information meeting the SWOG S0809 criteria were selected, 469 of whom received both adjuvant chemotherapy and radiotherapy. Median OS in patients undergoing chemoradiation was 36.9 months, and was not different between primary sites (p = 0.841). In a propensity score matched cohort, receipt of adjuvant chemoradiation had a survival benefit compared with adjuvant chemotherapy only (hazard ratio 0.86, 95% confidence interval 0.77–0.95; p = 0.004).

Conclusion
Using a large national database, we support the findings of SWOG S0809 with a similar median OS in patients receiving chemoradiation. These data further support the consideration of adjuvant multimodal therapy in resected biliary cancers.
Wybrane formy rzeźby terenu w sudeckiej części Geoparku Kraina Wygasłych Wulkanów (pd.-zach. Polska)
Authors: Migoń Piotr 
Published: March 2024

Obszar Geoparku Kraina Wygasłych Wulkanów obejmuje część górską (sudecką) i przedgórską. W granicach geoparku znajdują się część Gór Kaczawskich, mających cechy gór niskich oraz część Pogórza Kaczawskiego. Mimo niewielkich wysokości bezwzględnych, rzeźba terenu jest różnorodna pod względem morfologicznym i genetycznym, a zróżnicowanie w dużej mierze nawiązuje do złożonej budowy geologicznej obszaru. Wyróżniające się zespoły form to wzniesienia i grzbiety twardzielcowe, zbudowane ze skał wulkanicznych wieku permskiego i kenozoicznego oraz elementy rzeźby krawędziowej (kuesty) rozwiniętej na permskich i mezozoicznych skałach osadowych. Wśród wzniesień zbudowanych z bazaltów część ma charakter neków. Do odpornościowego zróżnicowania podłoża nawiązują także przełomy rzeczne. Georóżnorodność obszaru uzupełniają formy skałkowe i odziedziczone formy środowiska peryglacjalnego, osuwiska, formy krasowe, przełomy rzeczne związane z reorganizacją sieci odwodnienia po zaniku lądolodu skandynawskiego oraz formy antropogeniczne, głównie związane z działalnością górniczą. Liczne miejsca wyróżniające się pod względem rzeźby mają charakter geostanowisk i są zagospodarowane geoturystycznie lub planowane do takiego zagospodarowania w przyszłości.
Rola łączności sieci dolinnej, rzecznej i drogowej w transporcie materiału zawieszonego w zlewni rolniczej i zalesionej (Pogórze Wiśnickie, Polska)
Authors: Święchowicz Jolanta , Natanek Adrianna 
Published: March 2024

Poznanie źródeł osadów, szlaków i dynamiki transportu oraz czynników, które leżą u podstaw przestrzennej i czasowej zmienności w transporcie zawiesiny w rzekach jest nadal kluczowym zagadnieniem poznawczym w geomorfologii. W zlewni nakładają się na siebie trzy sieci umożliwiające transport wody i osadów. Są to naturalne sieci dolinna i rzeczna oraz związana z gospodarczą działalnością człowieka sieć drogowa. Sieć dolinna, rzeczna i drogowa są ze sobą połączone, a w miejscach tych połączeń może następować dostawa materiału do transportu rzecznego. Celem pracy jest poznanie roli sieci dolinnej, rzecznej i drogowej w transporcie zawiesiny w zlewni rolniczej i zalesionej. Podstawą opracowania są badania przeprowadzone w zlewniach Dworskiego Potoku i Leśnego Potoku położonych na Pogórzu Wiśnickim. W rolniczej zlewni Dworskiego Potoku wyróżniono 10 węzłów: ciek–ciek, ciek–dolina, dolina–dolina i droga–droga. Najwięcej skrzyżowań występuje pomiędzy dolinami (połączenie dolina–dolina) oraz pomiędzy dolinami i ciekami (połączenie ciek–dolina). W zalesionej zlewni Leśnego Potoku wyróżniono 497 węzłów: ciek–ciek, ciek–dolina, ciek–droga, dolina–dolina i droga–droga. Najwięcej węzłów występuje pomiędzy dolinami (połączenie dolina–dolina), następnie między drogami (połączenie droga–droga) oraz między dolinami i ciekami wodnymi (połączenie ciek–dolina). Mniej powszechne są połączenia dróg i dolin oraz dróg i cieków wodnych. Najmniej połączeń występuje pomiędzy ciekami wodnymi.
Makro- i mikrożużle w aluwiach równiny zalewowej Kamionki koło Jędrowa (Góry Świętokrzyskie) jako wskaźnik dawnej działalności metalurgicznej
Authors: Przepióra Paweł , Kalicki Tomasz , Houbrechts Geoffrey 
Published: March 2024

Kamionka jest rzeką trzeciego rzędu i prawostronnym dopływem Kamiennej (Wyżyna Kielecka). W całej zlewni w ramach Staropolskiego Okręgu Przemysłowego rozwijał się przemysł metalurgiczny oraz młynarski. Młyn w Jędrowie (Suchedniów) jest jednym z niewielu obiektów na terenie Staropolskiego Okręgu Przemysłowego, który zachował się w dobrym stanie, włączając w to również pozostałości wciąż widocznej w rzeźbie infrastruktury hydrotechnicznej m.in. dawnej kuźnicy. Dotychczasowe badania w zlewni wykazały występowanie licznych śladów działalności metalurgicznej zachowanych w osadach równiny zalewowej Kamionki oraz dawnych stawów przemysłowych. Większość pozostałości po kuźnicy działającej w miejscu obecnego młyna reprezentuje żużel występujący m.in. w osadach jeziornych oraz na pobliskiej hałdzie. Wskaźnikiem średniowiecznego, a zwłaszcza nowożytnego hutnictwa na badanym stanowisku są również znajdowane w aluwiach węgielki drzewne datowane na ostatnie stulecia. Uzyskane wyniki potwierdzają liczne materiały historyczne opisujące dzieje tego miejsca. Oprócz makroskopowych fragmentów żużla odkryto również mikrosferulki występujące w aluwiach, które są produktem kucia żelaza w kuźnicy. Analiza z wykorzystaniem metody separacji magnetycznych sferulek żelaza (Magnetic Spherule Separation), dotychczas z powodzeniem stosowana była na rzekach ardeńskich. Po raz pierwszy użyto jej w badaniach rzek Gór Świętokrzyskich, w tym Kamionki w pobliżu Jędrowa. Separacja i pomiar tych artefaktów pozwala określić m.in. tempo akumulacji osadów pozakorytowych. Makro- i mikrożużle tworzą wyraźną warstwę przemysłową, która świadczy o akumulacji tych osadów w okresie funkcjonowania kuźnicy lub krótko po jej wygaszeniu. Zestawienie tych wyników z materiałami historycznymi pozwala na ustalenie wieku aluwiów oraz umożliwia rozpoznanie ewolucji równiny zalewowej w ostatnich stuleciach.
Epidemiology and burden of respiratory syncytial virus in Italian adults: A systematic review and meta-analysis
Authors: Domnich Alexander , Calabrò Giovanna Elisa 
Published: March 2024

Objective
Respiratory syncytial virus (RSV) is a common respiratory pathogen not only in children, but also in adults. In view of a recent authorization of adult RSV vaccines in Italy, our research question was to quantify the epidemiology and burden of RSV in Italian adults.

Methods
Observational studies on the epidemiology and clinical burden of laboratory-confirmed or record-coded RSV infection in Italian adults of any age were eligible. Studies with no separate data for Italian adults, modeling and other secondary publications were excluded. A literature search was performed in MEDLINE, Biological Abstracts, Global Health, Scopus and Web of Science on 22 November 2023. Critical appraisal was performed by means of a Joanna Briggs Institute checklist. Random-effects (RE) meta-analysis was performed to obtain pooled estimates and the observed heterogeneity was investigated by subgroup and meta-regression analyses. The protocol was prospectively registered (doi.org/10.17504/protocols.io.5qpvo32odv4o/v1).

Results
Thirty-five studies were identified, most of which had at least one possible quality concern. RSV seasonal attack rates ranged from 0.8 ‰ in community-dwelling older adults to 10.9% in hematological outpatients. In the RE model, 4.5% (95% CI: 3.2–5.9%) of respiratory samples tested positive for RSV. This positivity prevalence was higher in older adults (4.4%) than in working-age adults (3.5%) and in outpatient (4.9%) than inpatient (2.9%) settings. According to the meta-regression, study location and sample size were also significant predictors of RSV detection frequency. The pooled estimate of in-hospital mortality was as high as 7.2% (95% CI: 4.7–10.3%). Data on other indicators of the diseases burden, such as complication and hospitalization rates, were unavailable.

Conclusion
RSV poses a measurable burden on Italian adults, especially those of older age and with some co-morbidities. However, several data on the natural history of RSV disease are missing and should be established by future large-scale studies.
Ganzheitliches Biodiversitätsmanagement in der Baustoffindustrie
Authors: Heinrich Anneli , Marken Gesa 
Published: March 2024

Der Biodiversitätsverlust ist nicht nur eine Herausforderung für die Gesellschaft; auch Unternehmen – besonders der Baustoffindustrie – kommt eine Verantwortung für den Biodiversitätsschutz zu. Welche Lösungsansätze gibt es für die Branche? Welches Potenzial hat sie und wie sehen die Erfolgsfaktoren für die Umsetzung eines ­Bio­diversitätsmanagements aus?
Klimapolitisches Engagement auf kommunaler Ebene in Deutschland – Entwicklung eines Index zu Bewusstsein, Konzeption und Durchführung von Klimaschutz und Klimawandelanpassung
Authors: Tafel Julian , Zorn Anika , Schäfer Susann 
Published: March 2024

This paper presents an additive index on climate policy engagement at the municipal level, based on the three dimensions awareness, conception and implementation of climate change mitigation and adaptation. The distortion of the index is based on the self-perception and personal assessment of municipal decision-makers, which were collected and evaluated as part of a primary survey (postal survey). The index shows how strongly a municipality is committed to climate policy. Thus, it represents an extension of previous indices that either only consider climate mitigation or adaptation or are only based exclusively on secondary statistics. The second part of the paper describes the application of the index in three counties or 51 municipalities in Saxony, Saxony-Anhalt and Thuringia. The interpretation takes place in the context of structural characteristics of the municipalities concerned (number of inhabitants, share of votes of different parties, geographical location). The distribution of the index values shows a similar awareness in climate protection and climate change adaptation, but an increasing discrepancy in the implementation of adaptation-specific measures.