bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sniping at web applications to discover input-handling vulnerabilities
Authors: Brandi Ciro , Perrone Gaetano , Romano Simon Pietro 
Published: April 2024

AbstractWeb applications play a crucial role in modern businesses, offering various services and often exposing sensitive data that can be enticing to attackers. As a result, there is a growing interest in finding innovative approaches for discovering vulnerabilities in web applications. In the evolving landscape of web security, the realm of fuzz testing has garnered substantial attention for its effectiveness in identifying vulnerabilities. However, existing literature has often underemphasized the nuances of web-centric fuzzing methodologies. This article presents a comprehensive exploration of fuzzing techniques specifically tailored to web applications, addressing the gap in the current research. Our work presents a holistic perspective on web-centric fuzzing, introduces a modular architecture that improves fuzzing effectiveness, demonstrates the reusability of certain fuzzing steps, and offers an open-source software package for the broader security community. By addressing these key contributions, we aim to facilitate advancements in web application security, empower researchers to explore new fuzzing techniques, and ultimately enhance the overall cybersecurity landscape.
Long-term imaging and spatio-temporal control of living cells using targeted light based on closed-loop feedback
Authors: Wijewardhane Neshika , Denniss Ana Rubio , Uppington Matthew , Hauser Helmut , Gorochowski Thomas E. , Piddini Eugenia , Hauert Sabine 
Published: April 2024

AbstractThe ability to optically interact with cells on both an individual and collective level has applications from wound healing to cancer treatment. Building systems that can facilitate both localised light illumination and visualisation of cells can, however, be challenging and costly. This work takes the Dynamic Optical MicroEnvironment (DOME), an existing platform for the closed-loop optical control of microscale agents, and adapts the design to support live-cell imaging. Through modifications made to the imaging and projection systems within the DOME, a significantly higher resolution, alternative imaging channels and the ability to customise light wavelengths are achieved (Bio-DOME). This is accompanied by an interactive calibration procedure that is robust to changes in the hardware configuration and provides fluorescence imaging (Fluoro-DOME). These alterations to the fundamental design allow for long-term use of the DOME in an environment of higher temperature and humidity. Thus, long-term imaging of living cells in a wound, with closed-loop control of real-time frontier illumination via projected light patterns, is facilitated.
Bilateral adrenal neuroblastoma: peculiar pattern of a rare pediatric presentation
Authors: Fawzy Mohamed , Ahmed Gehad , Youssef Yasser , Elkinaai Naglaa , Refaat Amal , Elahmadawy Mai Amr , Said Fadwa , Elmenawi Salma 
Published: April 2024

Abstract
Background
Bilateral suprarenal neuroblastoma (BSN) is a rare presentation. Few previously published literature showed BSN patients to have favorable pattern and prognosis. This study aim was to evaluate clinical and biological features in relation to outcome of Egyptian patients with BSN.

Methods
Included patients were diagnosed from 2007 to 2017, retrospectively. Tissue biopsy, imaging and bone marrow were evaluated at presentation. Clinical, demographic, biological variables and risk group were determined and analyzed in relation to overall (OS) and event-free-survival (EFS).

Results
BSN patients (n = 33) represented 2% of hospital patients with neuroblastoma during the 10-year study period, 17 were males and 16 were females. Twenty-four patients (72.7%) were infants, and 9 patients (27.3%) were above 1 year of age (range: 1 month to 3 years). Metachronous disease was present in only one patient. Amplified MYCN was found in 10 patients. Initially, most patients (n = 25) had distant metastasis, 6 had stage 3 versus 2 stage 2. Fifteen were high risk (HR), 15 intermediate (IR), 1 low risk (LR) and 2 were undetermined due to inadequate tissue biopsy. Three-year OS for HR and IR patients were 40.5% and 83.9% versus 23.2% and 56.6% EFS; respectively.

Conclusion
BSN treatment is similar to unilateral disease. A more conservative surgical approach with adrenal tissue preservation on less extensive side should be considered. Biological variables and extent of disease are amongst the most important prognostic determinants. Future studies are warranted to further address the biologic profiling of BSN and highlight prognostic significance of size difference between both adrenal sides.
Diagnostic value of circulating tumor cells in patients with thyroid cancer: a retrospective study of 1478 patients
Authors: Zeng Qingxin , Zhong Haifeng , Rao Hui , Wang Yuedong 
Published: April 2024

Abstract
Background
Circulating tumor cell (CTC) detection is one form of liquid biopsy. It is a novel technique that is beginning to be applied in the field of thyroid cancer. The present study was designed to evaluate the diagnostic value of CTCs in patients with thyroid cancer.

Methods
A total of 1478 patients were retrospectively analyzed and divided into malignant group (n = 747) and benign group (n = 731). Peripheral blood was collected, and CTCs were enriched and quantified before surgery. The baseline data of the two groups were matched by Propensity Score Matching (PSM). Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic efficiency of different indicators for thyroid cancer. The malignant group before PSM was further divided into subgroups according to the BRAF V600E mutation and lymphatic metastasis (N stage), and the number of CTCs in different subgroups was compared.

Results
After 1:1 PSM, baseline characteristics of the malignant group and benign group were matched and assigned 315 cases in each group. The number of CTCs and the TPOAb values were comparable in the two groups (p > 0.05). The TgAb values [1.890 (1.110 – 16.010) vs 1.645 (1.030 – 7.073) IU/mL, p = 0.049] were significantly higher in the malignant group than in the benign group. After PSM, ROC analyses showed that the areas under the curve (AUCs) of CTC, TgAb and ultrasound were 0.537 (sensitivity 65.6%, specificity 45.8%), 0.546 (sensitivity 40.0%, specificity 70.8%) and 0.705 (sensitivity 77.1%, specificity 63.2%), respectively. The AUCs of the combined detection of ‘CTC + ultrasound’ (combine 1) and the combined detection of ‘CTC + TgAb + ultrasound’ (combine 2) were 0.718 (sensitivity 79.3%, specificity 61.7%) and 0.724 (sensitivity 78.0%, specificity 63.3%), respectively. The AUC of ultrasound was significantly higher than CTC (p < 0.001). There was no statistically significant difference in AUC between combination 1 and ultrasound, and between combination 2 and ultrasound (p > 0.05). The number of CTCs between the N0 and N1 subgroups, and between the BRAF mutant and BRAF wild subgroups was comparable (p > 0.05).

Conclusions
As an emerging and noninvasive testing tool, the efficacy of CTCs in diagnosing thyroid cancer is limited.
Biosynthesized copper oxide nanoparticles by Psidium guajava plants with antibacterial, antidiabetic, antioxidant, and photocatalytic capacity
Authors: Relhan Ankush , Guleria Samriti , Bhasin Aparajita , Mirza Anis , Zhou John L. 
Published: April 2024

AbstractWith an increasing focus on green technologies, this research aimed to synthesize copper oxide nanoparticles (CuO-NPs) using leaf extracts from Allahabad Safeda and Hisar Safeda for environmental and health protection. A range of concentrations of leaf extracts were employed in the synthesis of nanoparticles, utilizing 1–9% extract from Allahabad Safeda and 3–11% extract from Hisar Safeda. The synthesized CuO-NPs were characterized by UV–visible spectrophotometry, Dynamic light scattering, Fourier transform infrared spectroscopy, and Scanning electron microscope with energy-dispersive X-ray spectroscopy. CuO-NPs synthesized using 3% Allahabad Safeda extract and 5% Hisar Safeda extract exhibited a particle size of 15.88 nm and 14.05 nm, respectively. CuO-NPs synthesized with Allahabad Safeda extract exhibited superior antibacterial, antioxidant, antidiabetic, and photocatalytic properties. Their antibacterial tests demonstrated significant inhibition zones against Staphylococcus aureus (20.5 cm), Streptococcus latis (20.7 cm), Escherichia coli (19.5 cm), and Pseudomonas aeruginosa (19.7 mm). Additionally, CuO-NPs from Allahabad Safeda extract (70 µg/mL) exhibited 68.23% of scavenging activity against 2,2-diphenyl-1-picrylhydrazyl. Moreover, the same CuO-NPs at 100 µg/mL concentration showed 67.32% α-amylase inhibition and 75.18% α-glucosidase inhibition, confirming their antidiabetic activities. Furthermore, these nanoparticles demonstrated high performance in photocatalytic degradation, by degrading 82.31% methylene blue and 88.54% crystal violet within 150 min of UV irradiation. Overall, the findings highlight the feasibility of CuO-NPs synthesis using Allahabad Safeda extract and their potential applications in antibacterial treatment, combating diabetes, antioxidation, and environmentally friendly dye photodegradation process.
Graphical Abstract
Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration
Authors: Tao Chen-Yang , Wu Xiao-Ling , Song Shu-Shu , Tang Zheng , Zhou Yu-Fu , Tian Meng-Xin , Jiang Xi-Fei , Fang Yuan , Zhu Gui-Qi , Huang Run , Qu Wei-Feng , Gao Jun , Chu Tian-Hao , Yang Rui , Chen Jia-Feng , Zhao Qian-Fu , Ding Zhen-Bin , Dai Zhi , Zhou Jian , Liu Wei-Ren , Shi Ying-Hong , Fan Jia 
Published: April 2024

Abstract
Purpose
GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown.

Methods
Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hairpin RNA lentivirus was used to knock down GPX8, and the main signaling pathways were investigated using transcriptome sequencing and a phosphorylated kinase array. The sphere formation assays, cloning-formation assays and cell migration assays were used to evaluate the stemness and migration ability of HCC cells. Identifying the GPX8-interacting proteins was accomplished through immunoprecipitation and protein mass spectrometry.

Results
The GPX8 protein levels were downregulated in HCC patients. Low expression of GPX8 protein was related to early recurrence and poor prognosis in HCC patients. GPX8 knockdown could enhance the stemness and migration ability of HCC cells. Consistently, Based on transcriptome analysis, multiple signaling pathways that include the PI3K-AKT and signaling pathways that regulate the pluripotency of stem cells, were activated after GPX8 knockdown. The downregulation of GPX8 could increase the expression of the tumor stemness markers KLF4, OCT4, and CD133. The in vivo downregulation of GPX8 could also promote the subcutaneous tumor-forming and migration ability of HCC cells. MK-2206, which is a small-molecule inhibitor of AKT, could reverse the tumor-promoting effects both in vivo and in vitro. We discovered that GPX8 and the 71-kDa heat shock cognate protein (Hsc70) have a direct interaction. The phosphorylation of AKT encouraged the translocation of Hsc70 into the nucleus and the expression of the PI3K p110 subunit, thereby increasing the downregulation of GPX8.

Conclusion
The findings from this study demonstrate the anticancer activity of GPX8 in HCC by inactivating the Hsc70/AKT pathway. The results suggest a possible therapeutic target for HCC.
Gender Affirming Surgeries of the Trunk
Authors: Park Rachel H. , Stranix John T. 
Published: April 2024

AbstractPurpose of ReviewThis review aims to explore the current concept, techniques, outcomes and safety of gender affirming surgeries of the trunk for transgender and gender non-conforming individuals.Recent FindingsGender affirming surgery is increasingly becoming more accessible to patients with gender dysphoria. Chest surgery is the most popular form with various surgical techniques that one can utilize. It is critical for operating surgeons to understand the nuances of these procedures when compared to cis-gender body contouring procedures. Recent outcome data show that these procedures are safe and well tolerated with high patient satisfaction and mental health benefits. Further long-term outcome data are needed.SummaryGender affirming surgery of the trunk is a safe and well-tolerated treatment for patients with gender dysphoria with high patient satisfaction and mental health benefits.Graphical Abstract
Virtual Reality Relaxation for Mental Health Service Users with Complex Care Needs: a Pilot Study in Residential Care Homes and Supported Accommodation
Authors: Williams Grace , Drini Eugenia , Riches Simon 
Published: April 2024

AbstractVirtual reality (VR) relaxation is a promising, novel intervention for service users with a range of mental health conditions and has potential wellbeing benefits such as promoting relaxation and reducing stress and anxiety. Less is known about the implementation of VR relaxation for service users in complex care mental health services. This study aimed to investigate implementation of a single session of VR relaxation for service users in residential care homes and supported accommodation, who were supported by a South London community team. Participants could explore relaxing, natural environments in VR. Pre- and post-VR visual analogue scales of mood, immersiveness, helpfulness, narrative feedback and clinician reflections were recorded. Participants (N = 20) had psychotic disorders and other serious mental health conditions. Post-VR, there were significant increases in relaxation (large effect), happiness and connectedness to nature (both medium effects), and decreases in anxiety and sadness (both medium effects). Stress was low at baseline and there was no significant effect post-VR. Helpfulness and immersion scores were high (both > 8). Participants reported the intervention was very relaxing, exciting, uplifting and were enthusiastic about engaging in more VR relaxation and further psychological interventions, including therapy and other digital interventions. Participants reported finding a session facilitator helpful. Technical issues were minor and resolved by the facilitator. Findings indicate scope to widen access to VR relaxation within complex care services. More facilitators could offer multiple sessions to larger, more diverse groups of service users. Future research with more robust methodologies is needed to test effectiveness and longer-term impact.
Life Expectancy at Birth and Infant Mortality Rates of Indigenous Populations in Canada from 2004 to 2016
Authors: Dion Patrice , Tremblay Marilyn-Anne , Gagnon Roxanne , Sirag Elham 
Published: April 2024

AbstractLife expectancy and infant mortality rates are two important indicators of the well-being of a population. However, it is not possible to compute these indicators for specific Indigenous populations in Canada from vital statistics databases because information about the identity of individuals is lacking. We use alternative data sources, linkages between the long-form census questionnaire and the Canadian Vital Statistics, namely the Canadian Census Health and Environment Cohorts and the Canadian Birth Census Cohorts, to compute life expectancy at birth and infant mortality rates among Indigenous populations. We describe the data, explain the methodology, and analyze the results to demonstrate the usefulness of these databases for the regular reporting of these health outcomes and monitoring of trends.
Fortify the defense frontline: MAPKs phosphorylate receptor-like cytoplasmic kinase to maintain plant resistance in soybean
Authors: Rui Lu , Wang Wei 
Published: April 2024

AbstractMitogen-activated protein kinase (MAPK) activation is one of the significant immune events that respond to pathogens in plants. A MAPK cascade often contains a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK/MKK), and a MAPK. The well-characterized MAPK cascade, to date, is the MAPKKK3/4/5-MKK4/5-MPK3/6 module. Soybean cyst nematodes (SCN) is one of the most devastating soybean pathogens. However, the early immune components contributing to soybean resistance to SCN and the role of the MAPK cascade in the soybean–SCN interaction remain unclear. A recent study published in Plant Cell discovered that GmMPK3/6 phosphorylates a receptor-like cytoplasmic kinase (RLCK), CDG1-LIKE1 (GmCDL1), and maintains the stability of GmCDL1 in soybean. Remarkably, GmCDL1 enhances GmMPK3/6 activation and resistance to SCN by phosphorylating GmMAPKKK5 and activating the GmMAPKKK5-GmMKK4-GmMPK3/6 cascade. In addition, two L-type lectin receptor kinases (LecRKs), GmLecRK02g and GmLecRK08g, are involved in the GmCDL1 function after the perception of SCN. taken together, this study not only discovers a complete early immune pathway that responds to SCN infection in soybean, but also reveals a molecular mechanism by which plants maintain the activation of the MAPK cascade and resistance.
Inconsistent metaphysical dependence: cases from the Kyoto School
Authors: Casati Filippo , Fujikawa Naoya 
Published: April 2024

AbstractEven though metaphysical dependence has been a subject of a lively debate in contemporary metaphysics, it is rare in such a debate to seriously consider the possibility that the metaphysical dependence relations among the things in the reality is inconsistent. This paper focuses on two philosophers of the Kyoto School, Kitaro Nishida and Keiji Nishitani, who challenge the common supposition that the structure of reality is consistent. In this paper, we show that Nishida’s logic of place is a version of inconsistent foundationalism, according to which absolute nothingness as a foundational element does not depend on anything but depends on itself, and that Nishtani’s theory of the field of emptiness is a version of inconsistent coherentism, according to which emptiness does not depend on anything but depends on everything else (and possibly on itself).
Shear wave ultrasound elastography for estimating cartilage stiffness: implications for early detection of osteoarthritis
Authors: Georgas Elias , Rayes Adnan , Zhang Junhang , Zhou Qifa , Qin Yi-Xian 
Published: April 2024

AbstractCurrent osteoarthritis (OA) diagnosis relies on radiographic abnormalities found in later stages of the disease, posing a challenge to the treatment efficacy. Therefore, earlier detection of OA is essential for improving therapeutic outcomes. The aim of this study was to investigate the feasibility of shear wave ultrasound elastography (SWUE) to detect changes in cartilage mechanical properties under OA conditions ex-vivo. Bovine osteochondral units were harvested from femoral condyles and subjected to either trypsin degradation, cartilage surface roughness defect using varying degrees of sandpaper, or subchondral bone degeneration using formic acid (FA) injection. Shear waves were generated using a mechanical shaker, while a high-frequency ultrasound system operating at 18 MHz was employed to detect wave propagation along the samples. The elasticity of cartilage was estimated by the shear wave speed (SWS) through the auto-correlation method. Our results show that the estimated SWS of cartilage after 24, 48, and 72 hours of trypsin incubation significantly decreased by 37%, 43%, and 59%, respectively, compared to the control group. Surface roughness treatment using 150-grit sandpaper significantly decreased the SWS by 35% compared to the control. Samples treated with 7% FA showed a significant increase in SWS by 62%, 89%, and 53% compared to control, 1% FA, and 3% FA, respectively. Our findings demonstrate the feasibility of SWUE to differentiate the elastic properties of cartilage under different OA conditions. This study presents the potential of a noninvasive, nonionizing tool for early detection of OA, representing a significant step toward its clinical implementation.
Graphical Abstract
Effectiveness of a Fire Disaster PFA Simulation Game: A Single-Blinded Trial
Authors: Choi Yun-Jung , Song Heewon 
Published: April 2024

Abstract

Objective:
As societies become more complex, disasters are increasing in frequency and magnitude. To respond to the psychological problems that may arise in such situations, it is necessary to develop the psychological first aid (PFA) training program that is more engaging for disaster relief workers and less limited in time and space. This study aimed to investigate the effectiveness of a web-based PFA simulation game for disaster relief workers to provide to fire disaster victims.

Methods:
This was a non-randomized controlled experimental study with 30 participants in the experimental and control groups. The experimental group learned through the web-based PFA simulation game developed in this study, and the control group was provided with written educational materials regarding general disaster. The effects of time between groups and interaction between groups were tested.

Results:
Compared to the control group, the experimental group showed significant effects on core competencies in disaster response, self-efficacy, and problem-solving process, and the persistence of the effects was also significantly different.

Conclusion:
The web-based PFA simulation game was found to be effective in improving core competencies in disaster response, self-efficacy, and problem-solving process of disaster relief workers. These results suggest that simulation games can be an effective learning method for learning PFA for disaster relief workers. Since it is difficult to learn through direct participation in disaster situations, a web-based simulation game may be a more effective way to improve and maintain the competence of PFA.
Empirical evidence and computational assessment on design knowledge transferability
Authors: Rahman Molla H. , Bayrak Alparslan E. , Sha Zhenghui 
Published: April 2024

Abstract
Developing an artificial design agent that mimics human design behaviors through the integration of heuristics is pivotal for various purposes, including advancing design automation, fostering human-AI collaboration, and enhancing design education. However, this endeavor necessitates abundant behavioral data from human designers, posing a challenge due to data scarcity for many design problems. One potential solution lies in transferring learned design knowledge from one problem domain to another. This article aims to gather empirical evidence and computationally evaluate the transferability of design knowledge represented at a high level of abstraction across different design problems. Initially, a design agent grounded in reinforcement learning (RL) is developed to emulate human design behaviors. A data-driven reward mechanism, informed by the Markov chain model, is introduced to reinforce prominent sequential design patterns. Subsequently, the design agent transfers the acquired knowledge from a source task to a target task using a problem-agnostic high-level representation. Through a case study involving two solar system designs, one dataset trains the design agent to mimic human behaviors, while another evaluates the transferability of these learned behaviors to a distinct problem. Results demonstrate that the RL-based agent outperforms a baseline model utilizing the first-order Markov chain model in both the source task without knowledge transfer and the target task with knowledge transfer. However, the model’s performance is comparatively lower in predicting the decisions of low-performing designers, suggesting caution in its application, as it may yield unsatisfactory results when mimicking such behaviors.