bluuebunny/crossref_metadata_2025_split huggingface dataset:
The estimation of mean nuclear volume in the diagnosis of breast carcinoma
Authors: Neal Harold J. , Hurst Peter R. 
Published: February 2007

AbstractSixty‐two cases of breast pathology were randomly selected From the files of the Dunedin Public Hospital for evaluation of mean epithelial nuclear volume. The cases were comprised of both benign and malignant ductal epithelial disease, diagnosed in cytological smears or in histological sections. Nuclear volume in histological preparations was estimated by the stereological technique of point‐line intercept measurements to derive volume‐weighted mean volumes (vV). An index of the nuclear volume (Vi) of cytology smears was calculated from measurements of nuclear areas by either image analysis Vi(e) or point‐line intercepts Vi(p). By all methods of analysis a clear distinction of nuclear volume was found between the benign [means for the cytology were 148 μm3 (Vi(e)) or 246 μm3 (Vi(p)), and 203 μm3 (Vv) for the histology specimens] and malignant diseased cases [means for cytology: 524 μm3 (Vi(e)) or 886 μm3 (Vi(p)), and 587 μm3 (vV) for the histology specimens]. © 1992 Wiley‐Liss, Inc.
Cytopathology of malignant mesothelioma: A stepwise logistic regression analysis
Authors: Stevens Mark W. , Leong Anthony S‐Y. , Fazzalari Nicola L. , Dowling Kaylene D. , Henderson Douglas W. 
Published: February 2007

AbstractTwenty‐four cytologic features, previously reported to be useful in the distinction of malignant mesothelioma, adenocarcinoma, and benign mesothelial proliferation in serous effusions were assessed. Forty‐four cases of malignant mesotheliomas, 46 cases of metastatic adenocarcinomas, and 30 cases of benign mesothelial proliferations were examined for these parameters. When these cytologic features were subjected to a stepwise logistic regression analysis, five features were selected to distinguish malignant mesothelioma from adenocarcinoma. These were true papillary aggregates, multinucleation with atypia, cell‐to‐cell apposition, acinus‐like structures, and balloon‐like vacuolation, the latter two features being characteristic of adenocarcinoma. The four variables selected to distinguish malignant mesothelioma from benign mesothelial proliferations were nuclear pleomorphism, macronucleoli, cell‐in‐cell engulfment, and monolayer cell groups, the latter being a feature of benign proliferations. Using these selected variables, the logistic model correctly predicted 95.4% of cases of malignant mesothelioma versus 100% of adenocarcinoma and 100% of malignant mesotheliomas versus 90% of benign mesothelial proliferations. The results of regression analysis suggest that many of the previously described cytologic features are not important diagnostic discriminators. © 1992 Wiley‐Liss, Inc.
Aspiration cytodiagnosis of breast carcinoma in pregnancy and lactation with immunohistochemical and electron microscopic study of an unusual mammary malignancy with pleomorphic giant cells
Authors: Gupta Raj K. , Wakefield St John , Lallu Sharda , Fauck Robert , Simpson John , Dowle Carl S. 
Published: February 2007

AbstractWe have reviewed our experience with 8,706 needle aspiration cytology (NAC) of the breast which were done from January 1983 to June 1991, During our study we diagnosed four cases of breast carcinomas (three ductal type and one with pleomorphic giant cells) in pregnant or lactating women; in all of these the cytologic findings corresponded with the subsequent tissue diagnosis and cell block preparations. Considering that carcinoma of breast during pregnancy and lactation is rare and is second only to cervical cancer, it was felt that its diagnosis by NAC was useful for timely management. In the case in which pleomorphic giant cells were present as an integral component of the tumour, immunohistochemical and electron microscopic study was done. This is discussed in view of our recent experience with this unusual tumour.
Malignant rhabdoid tumor of soft tissues: A cytopathological and immunohistochemical study
Authors: Sola Pérez J. , Pérez‐Guillermo M. , Bas Bernal A. , Manzanera López T. , Caro López F. 
Published: February 2007

AbstractWe describe the cytopathological and immunohistochemical features of a primary malignant rhaboid tumor (MRT) located in the left forehead region of an 8‐mo‐old female. The cardinal characteristics are hyaline cytoplasmic inclusions, rounded or bean‐shaped nucleus, vesicular chromatin, central prominent nucleolus, and poorly defined cytoplasmic profile. Both the cytoplasm and the hyaline inclusion are vimentin and keratin positive, but negative with desmin, S‐100 protein, neuron‐specific enolase, (NSE) myoglobin, leukocyte common antigen (LCA), and alpha‐1‐antichymotrypsin. This immunohistochemical pattern, although not exclusive to MRT, is fairly typical of MRT, and when it is assessed in conjunction with cytopathological findings and clinical data permits a confident diagnosis of MRT. We emphasize the utility of immunohistochemical techniques in making the differential diagnosis of MRT against other childhood round‐Cell tumors. © 1992 Wiley‐Liss, Inc.
Needle aspiration cytology, immunocytochemistry, and electron microscopy in a rare case of secretory carcinoma of the breast in an elderly woman
Authors: Gupta Raj K. , Lallu Sharda D. , Fauck Robert , Simpson John S. , Wakefield St John 
Published: February 2007

AbstractNeedle aspiration was performed on a breast mass in a 91‐year‐old woman. The cytologic features in the aspirate were a diffuse, prominent, intracytoplasmic vacuolization and secretion in malignant cells and occasional signet ring‐like forms. This was confirmed in a subsequent cell block which was made from the aspirate. Immunocytochemical studies showed a positivity for mucin by alcian blue stain in the vacuolated cells which was periodic acid‐Schiff positive and resistant to diastase digestion. Oil‐red‐O staining was negative and on Colloidal iron stain the tumour cells were positive. Immunopositivity to carcinoembryonic antigen, cytokeratin, and epithelial membrane antigen was found in the malignant cells, while on electron microscopy the tumour cells contained a significant amount of intracytoplasmic secretory material. Secretory carcinoma of the breast is a rare tumour and can be diagnosed and differentiated from other breast carcinomas in view of its characteristic cytologic features. © 1992 Wiley‐Liss, Inc.
Evaluation of cytocentrifuge apparatus with special reference to the cellular recovery rate
Authors: Kobayashi Tadao K. , Ueda Masami , Yamaki Tarumi , Yakushiji Michiaki 
Published: February 2007

AbstractTwo types of commercially available cytocentrifuge apparatus (type A and type B apparatus) using disposable funnels were compared for percentage of cell recovery and degree of cell preservation. The cellularity of each cell suspension was determined using a Sysmex micro cell counter for blood analysis, and the cell recovery was obtained by counting cells in the total smeared area on the May‐Grünwald Giemsa (MGG)‐stained slide. Overall recovery rate by the type A apparatus was between 54.3% and 74.9% with a mean of 63.0%, whereas, the recovery rate for type B apparatus was between 30.6% and 51.8%, with a mean of 42.5%, indicating that the type A apparatus was significantly better. In the type A apparatus, a higher yield of all cells was obtained (69.7‐74.9%) in the group of low cell counts (350 cells/ 0.5 ml), which was run for 10 minutes at 2,000 rpm. On the other hand, in the type B apparatus a higher yield of all cells was obtained (38.6‐42.6%) in the group of low cell counts which was run for 10 minutes at 2,000 rpm. Cellular structure was better preserved on the slides in the type A apparatus. However, the percentage of ghost cells was somewhat higher in the type B apparatus. The cytocentrifugation of the type A apparatus consistently recovered a higher percentage of cells than with the type B apparatus.Using the type A apparatus, a high rate of cellular recovery, which is extremely important, such as for accurate morphological evaluation of cerebrospinal fluid, can be consistently obtained.
Fine‐needle aspiration cytology of localized fibrous tumor of pleura
Authors: Dusenbery David , Grimes Margaret M. , Frable William J. 
Published: February 2007

AbstractFine‐needle aspiration (FNA) biopsy findings are presented in three cases of localized fibrous tumor of pleura (LFTP). Clinically, two of the tumors behaved benignly, although one showed frequent mitoses and foci of necrosis. The third tumor exhibited local aggressiveness as evidenced by rib destruction. Cytologically, these tumors exhibited a wide range of cellularity, composed mainly of small, bland oval to spindle cells with moderate numbers of stripped nuclei. Small bits of collagen were seen in the smears. In all cases, cell blocks were instrumental in making the diagnosis. Previous cytologic descriptions of the tumor are briefly reviewed and the cytologic differential diagnosis is discussed. The peripheral location of this uncommon tumor makes it an ideal target for FNA biopsy. © 1992 Wiley‐Liss, Inc.
Role of fine‐needle aspiration cytology in the management of thyroid nodules: Review of experience with 1,925 cases
Authors: Bisi Hélio , De Camargo Rosalinda Y. Asato , Filho Adhemar Longatto 
Published: February 2007

AbstractIn this review article, the authors present their experience with the management of thyroid nodules using fine‐needle aspiration cytology as the primary method of investigation. © 1992 Wiley‐Liss, Inc.
Needle aspiration cytology, immunocytochemistry, and electron microscopic study of unusual pancreatic carcinoma with pleomorphic giant cells
Authors: Gupta Raj K. , Wakefield St John 
Published: February 2007

AbstractNeedle aspiration cytology, immunocytochemistry, and electronmicroscopic findings are presented in three cases of an unusual pancreatic carcinoma in which pleomorphic giant cells formed an integral part of the tumour. All three patients were elderly males (age range 66–83 years) and had pancreatic masses. Notable cytologic features in all cases were the presence of bizarre mononucleated and multinucleated, poorly cohesive tumour giant cells, rare spindle cells, with occasional cannibalism and cytophagocytosis. Immunocytochemical study of aspirated material showed diffuse staining of cytokeratin and EMA within the tumour cells, while B 72.3 was seen as focal trace stain. Electron microscopy of aspirated material demonstrated epithelial features and these were characterised by the presence of tonofilaments and surface microvilli. Based on our findings, it is felt that the bizarre giant cells in this unusual variant of pancreatic carcinoma are of epithelial origin. The differential diagnosis of other tumours that may be associated with predominant giant cells in pancreatic aspirates is appropriately discussed. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of malignant nerve sheath tumors
Authors: Vendraminelli R. , Cavazzana A. O. , Poletti A. , Galligioni A. , Pennelli N. 
Published: February 2007

AbstractPrecise preoperative diagnosis of three malignant nerve sheath tumors (MNST) was based on their remarkably uniform and highly characteristic cytologic appearance. The differential diagnosis with benign nerve sheath tumors and other spindle cell sarcomas is addressed, and the possibility of achieving confident diagnosis on cytologic material from at least some soft‐tissue sarcomas is confirmed. © 1992 Wiley‐Liss, Inc.
Comparative features of comedo and noncomedo ductal carcinoma in situ of the breast on fine‐needle aspiration biopsy
Authors: Malamud Yasmin R. , Ducatman Barbara S. , Wang Helen H. 
Published: February 2007

AbstractTo determine whether fine‐needle aspiration biopsy (FNAB) can differentiate between comedo (C‐DCIS) and noncomedo ductal carcinoma in situ (NC‐DCIS), we reviewed retrospectively the preoperative FNAB and surgical biopsy slides of 13 cases of DCIS with adequate cytologic material. Eight were NC‐DCIS and 5 were C‐DCIS. Three (60 percent) of the C‐DCIS and 7 (88%) of the NC‐DCIS were nonpalpable lesions biopsied under conventional mammographic guidance. Three (60%) of the C‐DCIS but only 2 (25%) of the NC‐DCIS were considered either suspicious or positive for malignancy on FNAB, the remainder in both groups being atypical. A statistically significant difference in marked nuclear pleomorphism (60% of C‐DCIS vs. 0% of NC‐DCIS, P=0.04) and large nucleoli (60% of C‐DCIS vs. 0% of NC‐DCIS, P=0.04) was observed between these 2 groups. DCIS is morphologically diverse, and it appears that the cytologic features of individual cells on FNAB may distinguish C‐DCIS from NC‐DCIS. Diagn Cytopalhol 1992;8:571‐576. © 1992 Wiley‐Liss, Inc.
Sinus histiocytosis with massive lymphadenopathy (Rosai‐Dorfman disease): Cytomorphologic analysis on fine‐needle aspirates
Authors: Schmitt Fernando C. 
Published: February 2007

AbstractCytomorphologic characteristics of smears from fine‐needle aspirates (FNA) of three cases of sinus histiocytosis with massive lymphadenopathy (SHML) (Rosai‐Dorfman disease) are described. All aspirates showed mature lymphocytes, plasma cells, and large pale histiocytes with well‐preserved lymphocytes within the cytoplasm (lymphocytophagocytosis). The cases were confirmed by surgical biopsy, and the immunohistochemical findings suggested a macrophage origin for this lesion. The value of FNA analysis as an aid to diagnosis of SHML is discussed.
Fine‐needle aspiration cytology of an endometrioid‐like variant of yolk sac tumor
Authors: Wade Strong J. , Frederick Worsham G. , Baker Angus S. , Hawk J. C. , Marshall Austin R. 
Published: February 2007

AbstractA 36‐year‐old male with a history of immature teratoma and embryonal carcinoma of the test is was admitted to the hospital for abdominal pain and fever. A CT scan revealed a large right abdominal mass. The patient's serum alpha‐fetoprotein (AFP) was 46.8 ng/ml (reference <25 ng/ml). Fine‐needle aspiration (FNA) of the mass revealed malignant glandular cells. Chemotherapy was instituted, followed by resection of the large abdominal mass. The tumor was grossly encapsulated, consisting of large areas of necrotic, hemorrhagic tissue surrounded by smaller, multiloculated cysts. Microscopically, the tumor had a villoglandular pattern and variably stratified tall columnar cells. A prominent feature of the columnar cells was supranuclear and subnuclear racuolization. Intracytoplasmic PAS‐positive, diastase‐resistant hyaline globules were occasionally present. AFP by immunoperoxidase was prominent within the tumor. This recurrence of the previously diagnosed testicular teratoma with embryonal carcinoma represents a yolk sac tumor with components strongly resembling endometrioid carcinoma, a variant only recently described in eight cases of ovarian origin (Clement et al: Am J Surg Pathol 1987;11(10):767‐778). We believe this is the first reported case of an endometrioid‐like variant of testicular yolk sac tumor and also the first report of the FNA cytology findings in this variant. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration of a periurethral teflon‐filled cyst following radical prostatectomy
Authors: Hanau Cheryl A. , Chancellor Michael B. , Alexander Archibald , Rishi Mazhar , Jordan Andrea G. 
Published: February 2007

AbstractPeriurethral Teflon injections are being used increasingly for the treatment of urinary incontinence after radical prostatectomy. We report a case of a man who developed increasing obstructive urinary symptoms and stress incontinence following radical retropubic prostatectomy. Six months earlier, he had undergone periurethral Teflon injections. On transrectal ultrasound and magnetic resonance imaging, a 3.2‐cm cystic lesion was noted at the prostatic bed near the bladder neck where the Teflon had been injected. Ultrasound‐guided transperineal fine‐needle aspiration of the cyst yielded a specimen with numerous birefringent crystalline Teflon particles. Although previous reports have described granulomatous tissue reaction, no multinucleated giant cells were present to suggest granuloma formation. To our knowledge, this is the first reported case of Teflon cyst formation following periurethral Teflon injections. The patient's history, imaging studies, cytopathology, and review of the literature are presented in this report. © 1992 Wiley‐Liss, Inc.
Infantile cutaneous rhabdomyosarcoma (Li‐Fraumeni syndrome): Cytological presentation of fine‐needle aspirate biopsy, report of a case
Authors: Pérez‐Guillermo M. , Bonmatí‐Limorte C. , García‐Rojo B. , Hernández‐Gil A. 
Published: February 2007

AbstractWe describe the cytopathological picture of a cutaneous rhabdomyosarcoma located in the left nasal furrow of a 4‐mo‐old girl, some of whose close relatives have died or suffered from different types of neoplasias (Li‐Fraumeni syndrome). We believe that the cytological picture is highly characteristic and rules out other round cell tumours of childhood. We underline the usefulness of FNAC in dermatology and strongly advocate the introduction of this technique into the diagnostic armoury of every dermatologist.
Detection of numerical chromosomal abnormalities in malignant cells on body fluids by fluorescence in situ hybridization of interphase cell nuclei with chromosome‐specific probes
Authors: Cajulis Ricardo S. , Frias‐Hidvegi Denise 
Published: February 2007

AbstractTo detect numerical chromosomal abnormalities (NCA) in malignant cells on body fluids, Fluorescence in situ hybridization (FISH) technique was tested in clinical specimens from patients with metastatic disease. Directly labeled DNA probes specific for chromosomes 8, 12, X, and Y (Imagenetics, Naperville, IL) were used for in situ hybridization to interphase cell nuclei. Fifteen body fluids (BF) from various sites were studied. Based initially on the Papanicolaou‐stained slides, there were seven malignant and eight benign samples. Blind analysis (200 cells/sample) showed that all benign samples had a normal number of chromosomes, whereas six of seven malignant samples showed different NCA comprising 5‐60% of the cell population ranging from three to 10 chromosome signals per cell. We conclude that interphase cytogenetic cell analysis of BF by FISH is: (1) feasible and gives superior signals for detection of NCA, (2) helpful in detecting malignant cells, (3) relatively simple with a turnaround time of less than 24 hr. This method may have diagnostic and prognostic application in the study of the biologic behavior of malignant neoplasms. © 1992 Wiley‐Liss, Inc.
Fibroadenoma of the breast: Diagnostic pitfalls of fine‐needle aspiration
Authors: Benoit Janine L , Kara Rosemin , Elizabeth McGregor S. , Duggan Máire A. 
Published: February 2007

AbstractOn fine‐needle aspiration (FNA), fibroadenomas have a characteristic cytological appearance, although occasional cases are misinterpreted as carcinomas and vice versa. In a review of 521 breast aspirates correlated with the subsequent histology, six of 87 fibroadenomas (7%) were malignant or suspicious of malignancy on FNA (false positives). Following cytological review, four were still suspicious of malignancy because of cellular dyscohesion and prominent nucleoli, while two were fibroadenomas. On FNA, four of 145 carcinomas (3%) were diagnosed as fibroadenomas (false negatives). On review, three were malignant or suspicious of malignancy, while one was consistent with a fibroadenoma. Three false negative diagnoses were due to underappreciation of single malignant cells present between epithelial groupings typical of a fibroadenoma, while one was due to undersampling of the carcinoma. Cytologically, some fibroadenomas are sufficiently atypical that histological confirmation is necessary to exclude a malignancy. Misinterpreting carcinomas as fibroadenomas could be avoided by careful study of the morphology of isolated cells.
Diagnosis of eosinophilic granuloma of bone by fine‐needle aspiration with concurrent institution of therapy: A cytologic, histologic, clinical, and radiologic study of 27 cases
Authors: Shabb Nina , Fanning Christina V. , Carrasco Cesar H. , Guo Shan Q. , Katz Ruth L , Ayala Alberto G. , Raymond A. Kevin , Cangir Ayten 
Published: February 2007

AbstractTwenty‐seven patients with eosinophilic granuloma (EG) of bone seen at our institution between 1979 and 1991 underwent fine‐needle aspiration (FNA) with or without concurrent Tru‐Cut biopsy. The 16 males and 11 females ranged in age from 2 1/2 to 61 years (median, 10 yr). Twenty‐four patients had monostotic lesions. The clinicoradiologic differential diagnosis included osteomyelitis and Ewing's sarcoma (young patients) and primary and metastatic malignancies (older patients). Twenty‐four of 28 FNAs (one patient had two FNAs) were diagnostic of EG, and 10 cases were diagnosed by FNA alone. Smears in these cases showed histiocytes, often with grooved or infolded nuclei, and abundant eosinophils. Multinucleated giant cells, foamy histiocytes, neutrophils, lymphocytes, and plasma cells were present in variable numbers. Four FNAs were misdiagnosed: two as osteomyelitis where smears contained abundant neutrophils, sparse eosinophils, and histiocytes misinterpreted as foamy histiocytes, and two as metastatic carcinoma (in adults) where histiocytes in a scant specimen (one case) and skin appendageal structures without lesional tissue (one case) were misinterpreted. These cases were correctly diagnosed on repeat FNA (one case), Tru‐Cut (two cases), or excisional biopsy (one case); however, three cases diagnosed by FNA had nondiagnostic concurrent Tru‐Cut biopsies. Treatment consisted of intralesional injection of 125 mg of methylprednisolone (22 cases). Progressive or complete healing of alt lesions occurred. FNA is a rapid and useful technique for the immediate diagnosis of EG that allows concurrent institution of therapy.
Eight cases of sister mary joseph's nodule diagnosed by fine‐needle aspiration
Authors: Fleming Marian V. , Oertel Yolanda C. 
Published: February 2007

AbstractWe report eight cases of umbilical metastases (Sister Mary Joseph's nodules) diagnosed by fine‐needle aspiration. The primary carcinoma was below the diaphragm in seven patients. There was one false‐negative diagnosis. We advocate the use of fine needle aspiration as the initial diagnostic test. It is a simple, fast, accurate, and relatively inexpensive means of making the definitive diagnosis. It can spare patients invasive procedures that are not clearly of benefit.
Objective comparison of cellular yield in fine‐needle biopsy of lymph nodes with and without aspiration
Authors: Cajulis Ricardo S. , Sneige Nour 
Published: February 2007

AbstractRecent reports have indicated that needle biopsy without aspiration obtained adequate material for diagnosis. To determine objectively the adequacy of cell yield both for diagnosis and for special studies (e.g., flow cytometry, markers), we prospectively studied 20 superficial lymph nodes in 20 patients with a history of lymphoma. In each case, the cytology materials were obtained by fine‐needle biopsy techniques with (FNAB) and without (FNB) aspiration. Two needle passes for each method were placed in separate tubes of RPMI medium. With FNAB, the cell counts (determined by a Coulter counter) ranged from 1,55 to 70 million (M) per tube, mean = 19.12 M, whereas with FNB, the cell counts ranged from 1.58 M to 40 M, mean = 15.48 M (P=0.4555). Although FNAB provided more cells in 12 cases (60%), FNB provided more cells in the remaining 8 cases (40%) (P=0.9392). Both techniques provided adequate numbers of cells for special studies in all cases. We conclude that FNB can provide an adequate number of cells both for diagnosis and for special studies.