bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artificial Intelligence–Generated Draft Replies to Patient Inbox Messages
Authors: Garcia Patricia , Ma Stephen P. , Shah Shreya , Smith Margaret , Jeong Yejin , Devon-Sand Anna , Tai-Seale Ming , Takazawa Kevin , Clutter Danyelle , Vogt Kyle , Lugtu Carlene , Rojo Matthew , Lin Steven , Shanafelt Tait , Pfeffer Michael A. , Sharp Christopher 
Published: March 2024

ImportanceThe emergence and promise of generative artificial intelligence (AI) represent a turning point for health care. Rigorous evaluation of generative AI deployment in clinical practice is needed to inform strategic decision-making.ObjectiveTo evaluate the implementation of a large language model used to draft responses to patient messages in the electronic inbox.Design, Setting, and ParticipantsA 5-week, prospective, single-group quality improvement study was conducted from July 10 through August 13, 2023, at a single academic medical center (Stanford Health Care). All attending physicians, advanced practice practitioners, clinic nurses, and clinical pharmacists from the Divisions of Primary Care and Gastroenterology and Hepatology were enrolled in the pilot.InterventionDraft replies to patient portal messages generated by a Health Insurance Portability and Accountability Act–compliant electronic health record–integrated large language model.Main Outcomes and MeasuresThe primary outcome was AI-generated draft reply utilization as a percentage of total patient message replies. Secondary outcomes included changes in time measures and clinician experience as assessed by survey.ResultsA total of 197 clinicians were enrolled in the pilot; 35 clinicians who were prepilot beta users, out of office, or not tied to a specific ambulatory clinic were excluded, leaving 162 clinicians included in the analysis. The survey analysis cohort consisted of 73 participants (45.1%) who completed both the presurvey and postsurvey. In gastroenterology and hepatology, there were 58 physicians and APPs and 10 nurses. In primary care, there were 83 physicians and APPs, 4 nurses, and 8 clinical pharmacists. The mean AI-generated draft response utilization rate across clinicians was 20%. There was no change in reply action time, write time, or read time between the prepilot and pilot periods. There were statistically significant reductions in the 4-item physician task load score derivative (mean [SD], 61.31 [17.23] presurvey vs 47.26 [17.11] postsurvey; paired difference, −13.87; 95% CI, −17.38 to −9.50; P &amp;lt; .001) and work exhaustion scores (mean [SD], 1.95 [0.79] presurvey vs 1.62 [0.68] postsurvey; paired difference, −0.33; 95% CI, −0.50 to −0.17; P &amp;lt; .001).Conclusions and RelevanceIn this quality improvement study of an early implementation of generative AI, there was notable adoption, usability, and improvement in assessments of burden and burnout. There was no improvement in time. Further code-to-bedside testing is needed to guide future development and organizational strategy.
Biomimetic Platelet‐Cloaked Nanoparticles for the Delivery of Anti‐Inflammatory Curcumin in the Treatment of Atherosclerosis
Authors: Fontana Flavia , Molinaro Giuseppina , Moroni Sofia , Pallozzi Giulia , Ferreira Mónica P. A. , Tello Rubén Pareja , Elbadri Khalil , Torrieri Giulia , Correia Alexandra , Kemell Marianna , Casettari Luca , Celia Christian , Santos Hélder A. 
Published: March 2024

AbstractAtherosclerosis still represents a major driver of cardiovascular diseases worldwide. Together with accumulation of lipids in the plaque, inflammation is recognized as one of the key players in the formation and development of atherosclerotic plaque. Systemic anti‐inflammatory treatments are successful in reducing the disease burden, but are correlated with severe side effects, underlining the need for targeted formulations. In this work, curcumin is chosen as the anti‐inflammatory payload model and further loaded in lignin‐based nanoparticles (NPs). The NPs are then coated with a tannic acid (TA)− Fe (III) complex and further cloaked with fragments derived from platelet cell membrane, yielding NPs with homogenous size. The two coatings increase the interaction between the NPs and cells, both endothelial and macrophages, in steady state or inflamed status. Furthermore, NPs are cytocompatible toward endothelial, smooth muscle and immune cells, while not inducing immune activation. The anti‐inflammatory efficacy is demonstrated in endothelial cells by real‐time quantitative polymerase chain reaction and ELISA assay where curcumin‐loaded NPs decrease the expression of Nf‐κb, TGF‐β1, IL‐6, and IL‐1β in lipopolysaccharide‐inflamed cells. Overall, due to the increase in the cell−NP interactions and the anti‐inflammatory efficacy, these NPs represent potential candidates for the targeted anti‐inflammatory treatment of atherosclerosis.
A Power‐Managed Tribovoltaic Nanogenerator and Self‐Powered Wireless Temperature Monitoring System
Authors: Cao Jie , Dong Zefang , Zhang Zhi , Gong Likun , Gao Yikui , Dai Yiming , Liu Guoxu , Feng Yuan , Jin Zhelin , Luan Ruifei , Wang Zhaozheng , Dong Sicheng , Cheng Guanggui , Zhang Chi , Ding Jianning 
Published: March 2024

AbstractThe emerging tribovoltaic nanogenerators (TVNGs) has garnered considerable attention in recent years owing to its higher power density, but it is still limited by unstable output, mismatched impedance, and lower charging speed. Herein, a power‐managed GaN‐based TVNG is first proposed and a self‐powered wireless temperature monitoring system (SWTMS) is developed. The energy backflow from transition capacitor to TVNG is effectively suppressed by applying a high‐voltage‐resistant diode. Benefiting from the combination of the maximum power point tracking (MPPT and the buck converter, an autonomous power management unit (PMU) is designed. The internal impedance of TVNG with PMU decreases by ten times and the charging speed of capacitor increases by 7.18 times compared to those without PMU. Moreover, 100 LEDs and six parallel thermohygrometers can be constantly powered. The wireless temperature monitoring in industrial environments without external power supply is demonstrated through the developed SWTMS. This work proposes an effective power management strategy for TVNG with high output voltage, which exhibits great potential to promote the development of self‐powered sensors and Industrial Internet of Things (IIoT).
United States crop conditions: 1986–2022
Authors: Bundy Logan R. , Gensini Vittorio A. , Ashley Walker S. , Haberlie Alex M. , Changnon David 
Published: March 2024

AbstractUSDA National Agricultural Statistics Service general crop condition data are used to quantify the relationship between crop conditions and yield, condition tendencies during the growing season, how conditions vary across the United States, and how conditions have trended for the 1986–2022 period for a variety of crops: barley (Hordeum vulgare L.), corn (Zea mays L.), cotton (Gossypium hirsutum L.), oats (Avena sativa L.), peanuts (Arachis hypogaea L.), rice (Oryza sativa L.), sorghum (Sorghum bicolor L.), soybeans (Glycine max L.), and spring and winter wheat (Triticum aestivum L.). Crop conditions are a statistically significant covariate (90% significance level) for yield and can be used in practice on a weekly basis. This becomes increasingly important as, historically, crop conditions not only deteriorated on average, but also became more variable throughout the growing season. Spatial differences in conditions, variability, and trends in condition ratings and variability are caused by crop selection, regional climate, and management practices such as irrigation. Crop conditions were poorest and had the highest interannual variability across the southern Great Plains region. Since 1986, crop conditions in the Great Plains region have deteriorated significantly, while variability has increased. Meanwhile, crop conditions across the southwestern and southeastern United States have significantly improved, while interannual variability in conditions has experienced robust declines. Given climatic and hydrologic trends, to maintain a positive trend in yield, continued advancements in production management and technology are required. Critical decisions regarding real‐time or future management can be supplemented by using crop condition risk assessment information along with actively monitoring weekly conditions.
Messagenin–based Chalcones: Synthesis, Modification and Perspectives of Antidiabetic Potency
Authors: Galimova Zarema , Khusnutdinova Elmira , Smirnova Irina , Poptsov Alexander , Spirikhin Leonid , Gabdrakhmanova Svetlana , Makara Nina , Nguyen Thi Thu Ha , Thi Tu Anh Le , Thanh Tra Nguyen , Thi Cham Ba , Kazakova Oxana 
Published: March 2024

AbstractA series of messagenin–based chalcones has been synthesized by Claisen‐Schmidt condensation and screened for in vitro α‐glucosidase inhibitory activity. The heterocyclization of 30‐(3‐pyridinylidene)‐messagenin led to syn/anti N‐acetyl‐pyrazoles in a ratio of 2 : 1 that were isolated by HPLC. Messagenin chalcones act as α‐glucosidase inhibitors with IC50 range from 0.055 to 80.70 μM. The lead nanomolar level α‐glucosidase inhibitor 30‐(4‐hydroxymethyl‐benzylidene)‐messagenin 10 was studied for antihyperglycemic activity on streptozotocin–induced diabetic animal models using in vivo mechanism–based assays. In a rat model of STZ‐induced T1DM, compound 10 (20 mg/kg, orally) exhibited antihyperlipidemic activity, reducing AIP (p>0.05) and CRI (p<0.05) compared to control. Compound 10 improved diabetic nephropathy, locomotor activity in rats, and reduced diabetes–related mortality.
Next‐Generation Metabolic Glycosylation Reporters Enable Detection of Protein O−GlcNAcylation in Living Cells without S‐Glyco Modification
Authors: Kufleitner Markus , Haiber Lisa Maria , Li Shuang , Surendran Harsha , Mayer Thomas U. , Zumbusch Andreas , Wittmann Valentin 
Published: March 2024

AbstractProtein O−GlcNAcylation is a ubiquitous posttranslational modification of cytosolic and nuclear proteins involved in numerous fundamental regulation processes. Investigation of O−GlcNAcylation by metabolic glycoengineering (MGE) has been carried out for two decades with peracetylated N‐acetylglucosamine (GlcNAc) and N‐acetylgalactosamine derivatives modified with varying reporter groups. Recently, it has been shown that these derivatives can result in non‐specific protein labeling termed S‐glyco modification. Here, we report norbornene‐modified GlcNAc derivatives with a protected phosphate at the anomeric position and their application in MGE. These derivatives overcome two limitations of previously used O−GlcNAc reporters. They do not lead to detectable S‐glyco modification, and they efficiently react in the inverse‐electron‐demand Diels–Alder (IEDDA) reaction, which can be carried out even within living cells. Using a derivative with an S‐acetyl‐2‐thioethyl‐protected phosphate, we demonstrate the protein‐specific detection of O−GlcNAcylation of several proteins and the protein‐specific imaging of O−GlcNAcylation inside living cells by Förster resonance energy transfer (FRET) visualized by confocal fluorescence lifetime imaging microscopy (FLIM).
Epoxide Ring‐Opening Reactions for Abundant Production of Mugineic Acids and Nicotianamine Probes
Authors: Kayano Kimika , Tsutsumi Tomohiro , Murata Yoshiko , Ogasa Chie , Watanabe Takehiro , Sato Ryota , Karanjit Sangita , Namba Kosuke 
Published: March 2024

AbstractA succinct synthetic approach to mugineic acids and 2’‐hydroxynicotianamine was established. Unlike all other synthetic methods, this approach utilized epoxide ring‐opening reactions to form two C−N bonds and is characterized by the absence of redox reactions. Mugineic acid was synthesized from three readily available fragments on a gram scale in 6 steps. The protected 2’‐hydroxynicotianamine was also synthesized in 4 steps, and the dansyl group, serving as a fluorophore, was introduced through a click reaction after propargylation of the 2’‐hydroxy group. The dansyl‐labeled nicotianamine (NA) iron complexes were internalized by oocytes overexpressing ZmYS1 (from maize) or PAT1 (from human) transporters, indicating successful transport of the synthesized NA‐probe through these transporters.
Covalent Organic Frameworks: Linkage Chemistry and Its Critical Role in The Evolution of π Electronic Structures and Functions
Authors: He Chunyu , Tao Shanshan , Liu Ruoyang , Zhi Yongfeng , Jiang Donglin 
Published: March 2024

AbstractCovalent organic frameworks (COFs) provide a molecular platform for designing a novel class of functional materials with well‐defined structures. A crucial structural parameter is the linkage, which dictates how knot and linker units are connected to form two‐dimensional polymers and layer frameworks, shaping ordered π‐array and porous architectures. However, the roles of linkage in the development of ordered π electronic structures and functions remain fundamental yet unresolved issues. Here we report the designed synthesis of COFs featuring four representative linkages: hydrazone, imine, azine, and C=C bonds, to elucidate their impacts on the evolution of π electronic structures and functions. Our observations revealed that the hydrazone linkage provides a non‐conjugated connection, while imine and azine allow partial π conjugation, and the C=C bond permits full π‐conjugation. Importantly, the linkage profoundly influences the control of π electronic structures and functions, unraveling its pivotal role in determining key electronic properties such as band gap, frontier energy levels, light absorption, luminescence, carrier density and mobility, and magnetic permeability. These findings highlight the significance of linkage chemistry in COFs and offer a general and transformative guidance for designing framework materials to achieve electronic functions.
Chiral Osmium(II)/Salox Species Enabled Enantioselective γ‐C(sp3)−H Amidation: Integrated Experimental and Computational Validation For the Ligand Design and Reaction Development
Authors: Chen Weijie , Xu Huiying , Liu Fu‐Xiaomin , Chen Kaifeng , Zhou Zhi , Yi Wei 
Published: March 2024

AbstractHerein, multiple types of chiral Os(II) complexes have been designed to address the appealing yet challenging asymmetric C(sp3)−H functionalization, among which the Os(II)/Salox species is found to be the most efficient for precise stereocontrol in realizing the asymmetric C(sp3)−H amidation. As exemplified by the enantioenriched pyrrolidinone synthesis, such tailored Os(II)/Salox catalyst efficiently enables an intramolecular site‐/enantioselective C(sp3)−H amidation in the γ‐position of dioxazolone substrates, in which benzyl, propargyl and allyl groups bearing various substituted forms are well compatible, affording the corresponding chiral γ‐lactam products with good er values (up to 99 : 1) and diverse functionality (>35 examples). The unique performance advantage of the developed chiral Os(II)/Salox system in terms of the catalytic energy profile and the chiral induction has been further clarified by integrated experimental and computational studies.
Modification of Polysulfide Surfaces with Low‐Power Lasers
Authors: Mann Abigail K. , Lisboa Lynn S. , Tonkin Samuel J. , Gascooke Jason R. , Chalker Justin M. , Gibson Christopher T. 
Published: March 2024

AbstractThe modification of polymer surfaces using laser light is important for many applications in the nano‐, bio‐ and chemical sciences. Such capabilities have supported advances in biomedical devices, electronics, information storage, microfluidics, and other applications. In most cases, these modifications require high power lasers that are expensive and require specialized equipment and facilities to minimize risk of hazardous radiation. Additionally, polymer systems that can be easily modified by lasers are often complex and costly to prepare. In this report, these challenges are addressed with the discovery of low‐cost sulfur copolymers that can be rapidly modified with lasers emitting low‐power infrared and visible light. The featured copolymers are made from elemental sulfur and either cyclopentadiene or dicyclopentadiene. Using a suite of lasers with discreet wavelengths (532, 638 and 786 nm) and powers, a variety of surface modifications could be made on the polymers such as controlled swelling or etching via ablation. The facile synthesis and laser modification of these polymer systems were exploited in applications such as direct laser lithography and erasable information storage.
A healing place: Reimagining the modern botanic garden as a physic garden
Authors: Entwisle Tim 
Published: March 2024

AbstractI have long promoted botanic gardens as a potent mix of nature, culture and science. Triggered by the COVID‐19 pandemic and the community's changing relationship with outdoor spaces, I want to add another vital element: the botanic garden as a reimagined physic garden. Not just a collection of medicinal plants, but a healing place. The value of nature, of gardens, and particularly of botanic gardens, for keeping us in “good health” is, if not self‐evident, then progressively confirmed by social research. The modern botanic garden as a physic garden embraces the creation and further development of botanic gardens in places where there are more people than “natural” diversity of plants; encourages a medical approach to addressing complex problems like climate change—targeting the root causes first, curing if possible, or if not, relieving symptoms and build resistance; favors a triaging approach to conservation for more effective use of resources; and provides plant rescue and restoration as the equivalent of a human Intensive Care Unit, with “plant ambulances” dispatched after natural (including human‐induced) disasters. Our landscapes and collections are for many their first contact with a complex plant environment—nature. That contact is also good for our health.
Superfetation and heterotopic pregnancy: Case report of two rare phenomena coexisting and implications in the era of assisted reproductive technologies
Authors: Badran Merhan , Labib Mazed , Abouali Omar , Pokhrel Prakriti 
Published: March 2024

Key Clinical MessageTo raise awareness about the increasing incidence of superfetation and heterotopic pregnancy in patients with ovarian induction, their insidious symptoms of abdominal pain, anemia, and hemodynamic instability in early pregnancy, and the usefulness of transvaginal ultrasound (TVUS) and quantitative beta human chorionic gonadotrophin (b‐hCG) for diagnosis.AbstractSuperfetation, occurrence of ovulation, fertilization, and implantation during an ongoing pregnancy and heterotopic pregnancy (HP) simultaneous presence of intrauterine and extrauterine pregnancies are infrequent phenomena. We report a case where both coexisted, challenges in diagnosis and management and association with the widespread use of assisted reproductive technologies (ARTs). A 32‐year‐old woman, who previously underwent ovulation induction therapy, presented with abdominal pain at 8 weeks pregnancy according to her last menstrual period. The patient had high quantitative serum beta‐human chorionic gonadotropin (b‐hCG) (30,883 mIU/mL). She was vitally stable and not anemic. Transvaginal ultrasound (TVUS) revealed two pregnancies at different gestational ages: an intrauterine pregnancy at 5 weeks and 3 days, and a right intact tubal ectopic pregnancy at 10 weeks and 5 days. Superfetation resulting in HP was then diagnosed. Subsequently, the patient underwent right laparoscopic salpingectomy. The intrauterine pregnancy progressed normally, resulting in delivery of a healthy full‐term neonate via Cesarean section at 38 weeks. Superfetation is typically rare from suppression of follicular development and ovulation during pregnancy. Various theories have been proposed to explain its etiology, including polyovulation, delayed blastocyst implantation, and abnormal estrogen and b‐hCG surges. In superfetation, an embryo resulting from a previous conception coexists with another embryo, either intrauterine, resulting in diamniotic dizygotic twins with significantly different gestational ages, or extrauterine resulting in HP. Despite being particularly challenging to diagnose because its presenting symptoms can overlap with those of other more common clinical conditions in early pregnancy, HP is increasingly seen with ARTs. In addition, the treatment of HP is versatile, ranging from expectant management to laparoscopic surgery. High level of suspicion for HP and superfetation is crucial in patients who, after ART, present with abdominal pain, hemodynamic instability, or anemia. Additionally, patients planning to undergo subsequent ART cycles should be thoroughly screened with b‐hCG and TVUS to exclude an ongoing intrauterine or extrauterine pregnancy.
Solid‐State Glass Nanopipettes: Functionalization and Applications
Authors: Wang Hao , Tang Haoran , Qiu Xia , Li Yongxin 
Published: March 2024

AbstractSolid‐state glass nanopipettes provide a promising confined space that offers several advantages such as controllable size, simple preparation, low cost, good mechanical stability, and good thermal stability. These advantages make them an ideal choice for various applications such as biosensors, DNA sequencing, and drug delivery. In this review, we first delve into the functionalized nanopipettes for sensing various analytes and the methods used to develop detection means with them. Next, we provide an in‐depth overview of the advanced functionalization methodologies of nanopipettes based on diversified chemical kinetics. After that, we present the latest state‐of‐the‐art achievements and potential applications in detecting a wide range of targets, including ions, molecules, biological macromolecules, and single cells. We examine the various challenges that arise when working with these targets, as well as the innovative solutions developed to overcome them. The final section offers an in‐depth overview of the current development status, newest trends, and application prospects of sensors. Overall, this review provides a comprehensive and detailed analysis of the current state‐of‐the‐art functionalized nanopipette perception sensing and development of detection means and offers valuable insights into the prospects for this exciting field.
A pH‐Sensitive Double Chromophore Fluorescent Dye for Live‐Tracking of Lipophagy
Authors: Engelhardt Pascal M. , Veronese Matteo , Eryiğit Alpay A. , Das Anushka , Kaczmarek Alexander T. , Rugarli Elena I. , Schmalz Hans‐Günther 
Published: March 2024

AbstractLipid droplet (LD) degradation provides metabolic energy and important building blocks for various cellular processes. The two major LD degradation pathways include autophagy (lipophagy), which involves delivery of LDs to autolysosomes, and lipolysis, which is mediated by lipases. While abnormalities in LD degradation are associated with various pathological disorders, our understanding of lipophagy is still rudimentary. In this study, we describe the development of a lipophilic dye containing two fluorophores, one of which is pH‐sensitive and the other pH‐stable. We further demonstrate that this “Lipo‐Fluddy” can be used to visualize and quantify lipophagy in living cells, in an easily applicable and protein label‐free approach. After estimating the ability of compound candidates to penetrate LDs, we synthesized several BODIPY and (pH‐switchable) rhodol dyes, whose fluorescence properties (incl. their photophysical compatibility) were analyzed. Of three Lipo‐Fluddy dyes synthesized, one exhibited the desired properties and allowed observation of lipophagy by fluorescence microscopy. Also, this dye proved to be non‐toxic and suitable for the examination of various cell lines. Moreover, a method was developed to quantify the lipophagy process using flow cytometry, which could be applied in the future in the identification of lipophagy‐related genes or in the screening of potential drugs against lipophagy‐related diseases.