bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENGEMBANGAN BAHAN AJAR MENULIS TEKS NEGOSIASI DENGAN MODEL DISCOVERY LEARNING BERBANTUAN E-BOOK DI KELAS XI MAS DDI AL MUJAHIDIN BENDORO
Authors: Masnawati  , Hanafi Muhammad , Ahmad Jamaluddin , Efendy Rasyid Rustam , Yusmah Yusmah , Adnan Andi Astina 
Published: February 2025

Penelitian ini bertujuan mendeskripsikan efektivitas bahan ajar yang dikembangkan terhadap kemampuan teks negosiasi dengan model discovery learning berbantuan e-book di kelas XI MAS DDI Al Mujahidin Bendoro. Penelitian ini merupakan penelitian pengembangan (Research and Development) yang diistilahkan dengan R&D.
Penelitian ini dilaksanakan di MAS DDI Al Mujahidin Bendoro Kabupaten Sidenreng Rappang yang direncanakan selama tiga bulan. populasi dalam penelitian ini adalah keseluruhan siswa kelas XI MAS DDI Al Mujahidin Bendoro yang berjumlah 62 orang. Sampel dalam penelitian ini ditarik secara keseluruhan atau sebanyak 63 orang. Teknik pengumpulan data yang dilakukan dalam penelitian ini adalah dokumentasi, observasi, angket, dan tes. Teknik analisis data dalam penelitian ini dilakukan dengan uji validasi dan N-Gain Score. Hasil penelitian menunjukkan bahwa bahan ajar yang dikembangkan di MAS DDI Al Mujahidin Bendoro berbentuk e-book yang mengintegrasikan model pembelajaran Discovery Learning. E-book ini terdiri dari komponen pendahuluan, isi materi, aktivitas discovery learning, serta latihan dan evaluasi, dengan fitur interaktif yang memungkinkan siswa belajar secara mandiri. Model Discovery Learning dirancang untuk mendorong eksplorasi aktif siswa dalam memahami konsep teks negosiasi. Berdasarkan hasil penelitian ini menunjukkan bahwa e-book yang dikembangkan dinilai mudah digunakan dengan respons positif dari siswa karena interaktivitas dan fleksibilitasnya. Efektivitas bahan ajar terlihat dari peningkatan nilai pretest dan posttest siswa dengan rata-rata n-Gain Score 0,3, yang termasuk kategori sedang hingga tinggi. Beberapa siswa menunjukkan peningkatan yang sangat signifikan dengan n-Gain Score lebih dari 0,7. Dengan demikian, e-book berbasis Discovery Learning ini terbukti efektif dalam meningkatkan kemampuan siswa menulis teks negosiasi.
ANALISIS PENGETAHUAN ETNOMATEMATIKA GURU DI SDN 018/VII MUARA TEBO
Authors: Antok Kurniawan  , Syahrul Ramadhan  
Published: February 2025

Penelitian ini bertujuan untuk menganalisis pengetahuan etnomatematika dan keterampilan mengajar menggunakan pendekatan etnomatematika guru sekolah dasar di SDN 018/VII Muara Tebo, implikasi pengetahuan etnomatika dan kendala-kendala yang dihadapi oleh guru dalam mengimplementasikan pengetahuan etnomatematika di sekolah dasar.Penelitian ini dilakukan di Kecamatan Tebo Tengah, yaitu SDN 018/VII Muara Tebo. Data penelitian diperoleh menggunakan dua pendekatan yaitu pendekatan kuantitatif dan pendekatan kualitatif. Penggunaan pendekatan kuantitatif dilakukan dengan cara menyebarkan angket kepada guru kelas, kemudian data angket tersebut dianalisis untuk mengetahui tingkat pengetahuan etnomatematika guru di sekolah dasar SDN 028/VII Muara tebo. Pendekatan kualitatif dilakukan dengan mewawancarai guru kelas dan analisis dokumentasi, data yang diperoleh kemudian dianalisis menggunakan teknik analisis Miles dan Hubermen. Hasil penelitian menunjukkan bahwa pengetahuan etnomatematika guru kelas di sekolah dasar di SDN 018/VII Muara Tebo. dari 5 responden tersebut mengetahui apa itu etnomatematika, sesuai dengan pernyataan dari narasumber MZ dan MH yang mengatakan bahwa istilah etnomatematika adalah pembelajaran matematika yang menghubungkan dengan kebudayaan. Narasumber NH dan AH mengatakan etnomatematika adalah pembelajaran yang mengaitkan dengan budaya. Diketahui bahwa masih ada guru yang belum menguasai teori tentang etnomatematika hal ini sesuai dengan pernyataan dari NZ yang mengatakan bahwa belum adanya penguasaan terhadap pembelajaran etnomatematika sehingga dalam proses pembelajaran belum menerapkan pembelajaran berbasis etnomatematika.
Middle Eastern and European Christianity, 16th-20th Century
Authors: Heyberger Bernard 
Published: February 2025

Bernard Heyberger carved new paths in the study of Middle Eastern Christianity, helping to shed fresh light on aspects of the connected history of the Near East that had previously been neglected. His approach to ‘global microhistory’ has focused on questions of space and circulation (people, texts, and objects) and his work has spanned many disciplines. In addition, he has made important contributions to the social and cultural history of Early Modern Catholicism.
This volume presents a collection of Heyberger’s studies in English for the first time, providing an unprecedented opportunity for the international public to access his work. The essays are accompanied by an introduction discussing the importance and legacy of his work and a comprehensive bibliography of his writings.
Secondary prevention of preeclampsia
Authors: Aldika Akbar Muhammad Ilham , Rosaudyn Roudhona , Gumilar Khanisyah Erza , Shanmugalingam Renuka , Dekker Gustaaf 
Published: February 2025

Preventing preeclampsia (PE) is crucial for the wellbeing of the mother, fetus, and the neonate with three levels: primary, secondary, and tertiary. Secondary prevention involves pharmacological therapies aimed at stopping the disease’s progression before clinical signs. The predominant approach currently employed is the daily administration of low dose Aspirin and calcium. PE is a multifaceted illness characterized by syncytiotrophoblast (STB) stress, leading to endothelial dysfunction and systemic inflammation. Various subtypes of PE, in particular early-onset PE (EOP) and late-onset PE (LOP), have different pathophysiological pathways leading to STB stress and also different perinatal outcomes. Low-dose Aspirin (LDA) has been shown to be beneficial in lowering the occurrence of EOP, especially when started before 16 weeks of pregnancy. Calcium supplementation is advantageous for women with poor dietary calcium intake, reducing endothelium activation and hypertension. Low molecular weight heparins (LMWH), have pleiotropic effects, besides their anticoagulant effects, LMWH have significant anti-inflammatory effects, and have a potential restricted use in patients with history of prior severe placental vasculopathy with or without the maternal preeclamptic syndrome. Pravastatin and other statins have shown positive results in lowering preterm PE and improving outcomes for both the mother and baby. Proton pump inhibitors (PPIs) have shown potential in lowering soluble FMS-like tyrosine kinase-1 (sFlt-1) levels and enhancing endothelial function, but clinical trials have been inconsistent. Metformin, primarily used for improving insulin sensitivity, has potential advantages in decreasing PE incidence due to its anti-inflammatory and vascular properties, particularly in morbidly obese women. Nitric oxide (NO) donors and L-arginine have been shown to effectively reduce vascular resistance and improving blood flow to placenta, potentially reducing PE risk. In conclusion, various pharmacological treatments have the potential to prevent secondary PE, but their effectiveness depends on underlying risk factors and intervention time. Further research is needed to determine the optimal (combination) of method(s) for the individual patient with her individual risk profile.
Systematic collection, annotation, and pattern analysis of viral vaccines in the VIOLIN vaccine knowledgebase
Authors: Huffman Anthony , Gautam Mehul , Gandhi Arya , Du Priscilla , Austin Lauren , Roan Kallan , Zheng Jie , He Yongqun 
Published: February 2025

BackgroundViral vaccines have been proven significant in protecting us against viral diseases such as COVID-19. To better understand and design viral vaccines, it is critical to systematically collect, annotate, and analyse various viral vaccines and identify enriched patterns from these viral vaccines. MethodsWe systematically collected experimentally verified viral vaccines from the literature, manually annotated, and stored the information in the VIOLIN vaccine database. The annotated information included basic vaccine names, pathogens and diseases, vaccine components, vaccine formulations, and their induced host responses. Enriched patterns were identified from our systematical analysis of the viral vaccines and vaccine antigens. ResultsA total of 2,847 viral vaccines against 95 viral species (including 72 RNA viral species and 23 DNA viral species) were collected, manually annotated, and stored in the VIOLIN vaccine database. These viral vaccines used 542 vaccine antigens. A taxonomical analysis found various DNA and RNA viruses covered by the viral vaccines. These vaccines target different viral life cycle stages (e.g., viral entry, assembly, exit, and immune evasion) as identified in top ranked human, animal vaccines, and HPV vaccines. The vaccine antigen proteins also show up in different virion locations in viruses such as HRSV vaccines. Both structural and non-structural viral proteins have been used for viral vaccine development. Protective vaccine antigens tend to have a protegenicity score of &gt;85% based on the Vaxign-ML calculation, which measures predicted suitability for vaccine use. While predicted adhesins still have significantly higher chances of being protective antigens, only 21.42% of protective viral vaccine antigens were predicted to be adhesins. Furthermore, our Gene Ontology (GO) enrichment analysis using a customized Fisher’s exact test identified many enriched patterns such as viral entry into the host cell, DNA/RNA/ATP/ion binding, and suppression of host type 1 interferon-mediated signaling pathway. The viral vaccines and their associated entities and relations are ontologically modeled and represented in the Vaccine Ontology (VO). A VIOLIN web interface was developed to support user friendly queries of viral vaccines. DiscussionViral vaccines were systematically collected and annotated in the VIOLIN vaccine knowledgebase, and the analysis of these viral vaccines identified many insightful patterns.
Assessment of platelet-to-white blood cell ratio on short-term mortality events in patients hospitalized with acute decompensated heart failure: evidence from a cohort study from Jiangxi, China
Authors: Huang Xin , Kuang Maobin , Qiu Jiajun , Wang Chao , Sheng Guotai , Zou Yang , Xie Guobo 
Published: February 2025

ObjectivePlatelet-to-white blood cell ratio (PWR) as a comprehensive indicator of inflammatory response has been widely used to assess the prognosis of various diseases. However, the relationship between PWR and adverse outcomes in patients with acute decompensated heart failure (ADHF) remains unclear. This study aimed to evaluate the association between PWR and all-cause mortality within 30 days of hospitalization in ADHF patients from Jiangxi, China.MethodsA total of 1,453 ADHF patients from the Jiangxi-ADHF study1 cohort were included. The primary outcome measure was all-cause mortality within 30 days of hospitalization. Multivariable Cox proportional hazards regression, restricted cubic spline regression, and receiver operating characteristic curve analysis were employed to explore the association between the inflammatory marker PWR and all-cause mortality in ADHF patients within 30 days of hospitalization.ResultsDuring the 30-day observation period, a total of 53 subjects experienced mortality events. Multivariable Cox regression showed a negative correlation between PWR and all-cause mortality within 30 days of hospitalization in ADHF patients. Restricted cubic spline regression demonstrated an L-shaped association between PWR and 30-day mortality risk (p for nonlinear = 0.038). Further threshold analysis revealed a threshold point for PWR at 15.88, where a decrease in PWR below this threshold was significantly associated with increased risk of all-cause mortality (p for log-likelihood ratio test = 0.046). Additionally, the results of receiver operating characteristic curve analysis indicated that PWR had high predictive accuracy for mortality events within 30 days of hospitalization in ADHF patients and is significantly better than the traditional HF marker N-Terminal Pro-Brain Natriuretic Peptide (AUC: NT-proBNP 0.69, PWR 0.76; Delong test P &lt; 0.05). Subgroup analysis showed that compared to subjects with reduced or moderately reduced ejection fraction, ADHF patients with preserved ejection fraction had a lower risk of short-term mortality associated with PWR (HR:0.99 vs. 0.98 vs. 0.87, P for interaction = 0.0067).ConclusionThis study reveals, for the first time, a negative correlation between the inflammatory marker PWR and all-cause mortality within 30 days of hospitalization in ADHF patients. Based on the threshold analysis findings, patients with ADHF and a PWR below 15.88 had a significantly higher risk of death within 30 days.
Weight loss-independent changes in human growth hormone during water-only fasting: a secondary evaluation of a randomized controlled trial
Authors: Horne Benjamin D. , Anderson Jeffrey L. , May Heidi T. , Bair Tami L. , Le Viet T. , Iverson Leslie , Knowlton Kirk U. , Muhlestein Joseph B. 
Published: February 2025

IntroductionWater-only fasting for one day or more may provide health benefits independent of weight loss. Human growth hormone (HGH) may play a key role in multiple fasting-triggered mechanisms. Whether HGH changes during fasting are independent of weight loss and how basal HGH and HGH change relate to other fasting-induced changes are unknown.MethodsApparently healthy individuals (N=30) were randomized by Latin square to begin two days with either 24-hour water-only fasting or a control of 24-hour ad libitum eating. On day 2, subjects were crossed over to control (if day 1 was fasting) or fasting (if they ate on day 1). HGH, weight, and other parameters were measured at baseline and at the end of the first and second days.ResultsBaseline HGH had median 0.50 ng/mL for females (n=20) and 0.04 ng/mL for males (n=10), and correlated inversely with weight, glucose, insulin, and triglycerides and positively with changes in insulin and HOMA-IR. The 24-hour fasting-induced HGH change was uncorrelated with weight loss (r= 0.01, p=0.98), but correlated with changes in glucose, HGB, and IGF-1. The percent increase in HGH was greater (p&lt;0.001) for lower (females ≤0.15 ng/mL, males ≤0.05 ng/mL) vs. higher baseline HGH (median: 1,225% vs. 50.3%, respectively). Subjects with lower baseline HGH had a trend to greater reduction of HOMA-IR (median: -6.15 vs. -1.35 for lower vs. higher HGH, respectively, p=0.08).ConclusionsFasting increased HGH and the HGH changes were independent of weight loss. Basal HGH and fasting-induced HGH changes correlated inversely with cardiometabolic risk factors.Clinical Trial Registration: clinicaltrials.gov, identifier NCT01059760.
The role of natural exosomes from SHED-MSC in immunoregulation of M0/M1 polarized macrophage cells
Authors: Fallah Ali , Hosseinzadeh Colagar Abasalt , Khosravi Ayyoob , Mohammad-Hasani Azadeh , Saeidi Mohsen 
Published: February 2025

IntroductionExosomes (EXOs) as a targeted cell-free therapy could offer a new therapeutic strategy for immune-mediated inflammatory diseases, due to their stability and ease of storage and handling. This study focused on exosomes derived from stem cells of human exfoliated deciduous teeth (SHED-MSC-EXOs) and their role in managing the balance of immunoregulatory macromolecules that play a role in the underlying immunoregulatory mechanisms in THP-1-derived M0/M1 macrophage cells.MethodsFlow cytometry confirmed the expression of CD14, CD68, CD80, and CD86 markers in these macrophages. Following morphological and survival assessments, culture supernatants from SHED-MSCs were used to isolate exosomes. Once the exosomes were verified, Calcein AM-labeled EXOs were introduced to the macrophage cells. The immunoregulatory macromolecules were assessed by analyzing surface markers, cytokine production, and pro- and antioxidant activity.ResultsMacrophages treated with exosomes exhibited immunomodulatory effects akin to those treated with dexamethasone. The levels of anti-inflammatory and antioxidant markers, including CD206, Arg-1, IL-10, TGF-β, TAC, CAT, and SOD, which act as immunosuppressive macromolecules, were elevated. In contrast, there was a reduction in pro-inflammatory and pro-oxidant markers, including CD80, CD81, IL-6R, IL-12, TNF-α, MDA, and NO, which act as immunostimulatory macromolecules (P &lt; 0.05).DiscussionThe findings suggest that exosomes derived from SHED-MSC can skew M0/M1 macrophages to the M2 phenotype and inhibit M1 polarization. These nanovesicles, with their distinct physical properties and ability to penetrate target cells, may prove beneficial in conditions involving the depletion of M2 macrophages and M1 macrophage-induced diseases, potentially aiding in the reduction of inflammation and tissue injury.
The efficacy of a low-dose combination of febuxostat and benzbromarone versus each drug used alone for the treatment of gout
Authors: Zheng Caiyu , Tong Qingwen , Zhang Zhijun , Lin Chunmei , Wang Zhiyi , Kang Dongshu , Lin Yanmei , Tian Jianqing 
Published: February 2025

ObjectiveThe study focuses on comparing the efficacy of a low-dose combination of febuxostat and benzbromarone versus each drug used alone for the treatment of gout.MethodsA prospective, randomized, open-labeled trial of men with gout and renal uric acid underexcretion was conducted. We randomly assigned 100 patients to either low-dose febuxostat or low-dose benzbromarone for initial treatment. 43 patients with complete data were analyzed in this study. The analysis of medication Treatment effects, Period effects and Patient-Within-Sequence effects for different treatment options was performed by cross-test ANOVA.ResultThe cross-trial analysis revealed no significant differences in the magnitude of uric acid decline among the three groups after treatment. Similarly, there were no statistically significant differences in blood lipid levels, CRP, CA724, eGFR, and ALT among the three groups post-treatment. The null model without participant-specific benefits was a linear mixed model for ALT, AST, eGFR, TC, TG, LDL, UA, CRP, CA724, and 24-h uric acid with age, BMI, treatment period and treatment as fixed factors, and the intercept as a random factor by participant. The results indicated no significant differences on relevant indicators among the three treatment regimens.ConclusionOur study did not identify a difference in the efficacy of reducing uric acid excretion in gout patients when comparing conventional dose febuxostat and benzbromarone monotherapy versus low-dose combination therapy.
Antifungal activity and mechanism of Phoebe bournei wood essential oil against two dermatophytes
Authors: Yang Yan , Sun Qinglin , Zhang Yuting , Huang Junhua , Ma Wenjun , Yang Qi , Tong Zaikang , Zhang Junhong 
Published: February 2025

BackgroundDermatophytes are notorious pathogenic fungi that threaten human health and reduce quality of life. Phoebe bournei (Hemsl.) Yen C. Yang wood essential oil (PWEO) has been found to have excellent antifungal activity, but its mechanism remains unclear.MethodologyDetermination of minimum inhibitory concentration (MIC) of PWEO on two dermatophytes (Microsporum gypseum and Epidermophyton floccosum) by broth microdilution method. Culture plates containing PWEO in vitro tested PWEO inhibition effect of mycelial growth of dermatophytes and the effect of PWEO on hyphal structure was observed by microscopy; the changes of cell membrane permeability and the degree of lipid peroxidation were reflected by measuring cell physiological indexes. ROS and MMP probe detection of intracellular ROS and MMP change. Finally, WGCNA analysis was used to identify and verify the key genes.ResultsWe found that the main components of PWEO are monoterpenes and sesquiterpenoids. The PWEO had strong antifungal activity, and the MIC of PWEO against both dermatophytes was 3.600 mg/mL. PWEO significantly inhibit mycelial growth, and the inhibitory effect increases significantly with increasing concentration. When the PWEO concentration reaches 1.8mg/mL, mycelial growth is completely inhibited. Microscopic observation showed that PWEO destroy the structure of hyphae. The cell membrane permeability test indicated that the cell membrane of dermatophytes was damaged by PWEO. Cellular malondialdehyde (MDA) content was positively correlated with the concentration of PWEO, suggesting that lipid peroxidation of dermatophytes was caused by PWEO. Fluorescence microscopy images showed excessive production of ROS and disruption of MMP in dermatophytes after PWEO treatment. Physiological experiment of M. gypseum showed significant differences in protein extravasation, extracellular conductivity and intracellular MDA content after three hours of treatment with 0.450 mg/mL PWEO compared with the control. Five hub genes were identified by weighted gene co-expression network analysis (WGCNA), of which Long chain fatty acid CoA ligase 1 (ACSL1) was significantly up-regulated expressed. Meiotically up-regulated 72 (MUG72) and GDP-mannose transporters gene 1 (GMT1) were significantly down-regulated expressed after PWEO treatment, which affected the growth and reproduction of M. gypseum. These results suggest that PWEO can be used as natural antifungal agents for sustainable applications.
Optineurin overexpression ameliorates neurodegeneration through regulating neuroinflammation and mitochondrial quality in a murine model of amyotrophic lateral sclerosis
Authors: Zhao Shumin , Chen Ranran , An Yi , Zhang Yali , Ma Cheng , Gao Ying , Lu Yanchao , Yang Fei , Bai Xue , Zhang Jingjing 
Published: February 2025

IntroductionAmyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the loss of motor neurons (MNs). Genetic mutations in Optineurin (OPTN) and Superoxide Dismutase 1 (SOD1) have been identified as causal factors for ALS. OPTN immunopositive inclusions have been confirmed in the cases of ALS with SOD1 mutations. However, the role of the OPTN gene in ALS caused by SOD1 mutations is ambiguous.MethodsThe murine Optn lentivirus and empty vector lentivirus were injected into SOD1G93A mice after discovering variations in Optn expression over time. The phenotype onset date, life span, locomotor activity, and pathological changes in the spinal cord were determined and recorded subsequently. In addition, the influences on cellular apoptosis, mitochondrial dynamics, mitophagy, and neuroinflammation were further investigated.ResultsOptn expression was increased in the spinal cord of SOD1G93A mice at the pre-symptomatic phase, but decreased after disease onset. Optn overexpression led to a 9.7% delay in the onset of disease and improved motor performance in SOD1G93A mice. Optn overexpression also ameliorated the MNs loss by 46.8%. Moreover, all these ameliorating effects induced by Optn overexpression might be due to the inhibition of cellular apoptosis, improvement of mitochondrial quality, regulation of mitochondrial dynamics, promotion of mitophagy, and anti-inflammatory properties.ConclusionOur data demonstrate that Optn overexpression protects MNs, inhibites cellular apoptosis, improves mitochondrial quality and regulates neuroinflamation in SOD1G93A mice at the pre-symptomatic stage.