bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standard chemotherapy with interferon compared with CHOP followed by high-dose therapy with autologous stem cell transplantation in untreated patients with advanced follicular lymphoma: the GELF-94 randomized study from the Groupe d'Etude des Lymphomes de l'Adulte (GELA)
Authors: Sebban Catherine , Mounier Nicolas , Brousse Nicole , Belanger Coralie , Brice Pauline , Haioun Corinne , Tilly Herve , Feugier Pierre , Bouabdallah Redah , Doyen Chantal , Salles Gilles , Coiffier Bertrand 
Published: July 2006

AbstractThe purpose of this study is to compare our standard chemotherapy regimen (CHVP [cyclophosphamide, doxorubicin, teniposide, and prednisone]) plus interferon with 4 courses of CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) followed by high-dose therapy with autologous stem cell transplantation (ASCT) in treatment-naive patients with advanced follicular lymphoma. Four hundred one patients were included from July 1994 to March 2001: 209 received 12 cycles of CHVP plus interferon α for 18 months (CHVP-I arm) and 192 received 4 cycles of CHOP followed by high-dose therapy (HDT) with total body irradiation and ASCT (CHOP-HDT arm). Overall response rates were similar in both groups (79% and 78% after induction chemotherapy, respectively). One hundred thirty-one of the 150 patients eligible for HDT underwent transplantation (87%). Intent-to-treat analysis after a median follow-up of 7.5 years showed that there was no difference between the 2 arms for overall survival (P = .53) or event-free survival (P = .11). Patients with a complete response at the end of the induction therapy had a statistically longer event-free survival and overall survival (P = .02 and < .001, respectively). After long-term follow-up, our study showed that there was no statistically significant benefit in favor of first-line high-dose therapy in patients with follicular lymphoma. High-dose therapy should be reserved for relapsing patients.
Endogenous thrombospondin-1 is a cell-surface ligand for regulation of integrin-dependent T-lymphocyte adhesion
Authors: Li Shu Shun , Liu Zhiwen , Uzunel Mehmet , Sundqvist Karl-Gösta 
Published: July 2006

AbstractLymphocyte adhesion to cells and extracellular matrix (ECM) via integrins plays a pivotal role for the function of the immune system. We show here that endogenous thrombospondin-1 (TSP-1) is a cell-surface ligand for cis interaction of surface receptors in T lymphocytes controlled by integrins and the T-cell antigen receptor (TCR/CD3). Stimulation of CD3 triggers rapid surface expression of TSP-1 in quiescent T cells, whereas activated cells express TSP-1 constitutively. Endogenous TSP-1 is attached to lipoprotein receptor-related protein 1 (LRP1/CD91) and calreticulin (CRT) on the cell surface through its NH2-terminal domain. Adhesion via integrins to ICAM-1 or ECM components up-regulates TSP turnover dramatically from a low level in nonadherent cells, whereas CD3 stimulation inhibits TSP turnover through interference with CD91/CRT-mediated internalization. Integrin-associated protein (IAP/CD47) is essential for TSP turnover and adhesion through interaction with the C-terminal domain of TSP-1 in response to triggering signals delivered at the NH2-terminal. These results indicate that endogenous TSP-1 connects separate cell-surface receptors functionally and regulates T-cell adhesion.
Treatment of solid organ transplant recipients with autologous Epstein Barr virus–specific cytotoxic T lymphocytes (CTLs)
Authors: Savoldo Barbara , Goss John A. , Hammer Markus M. , Zhang Lan , Lopez Teresita , Gee Adrian P. , Lin Yu-Feng , Quiros-Tejeira Ruben E. , Reinke Petra , Schubert Stephan , Gottschalk Stephen , Finegold Milton J. , Brenner Malcolm K. , Rooney Cliona M. , Heslop Helen E. 
Published: July 2006

AbstractWe have investigated the in vivo safety, efficacy, and persistence of autologous Epstein Barr virus (EBV)–specific cytotoxic T lymphocytes (CTLs) for the treatment of solid organ transplant (SOT) recipients at high risk for EBV-associated posttransplantation lymphoproliferative disease (PTLD). EBV-CTLs generated from 35 patients expanded with normal kinetics contained both CD8 and CD4 lymphocytes and produced significant specific killing of autologous EBV-transformed B lymphoblastoid cell lines (LCLs). Twelve SOT recipients at high risk for PTLD, or with active disease, received autologous CTL infusions without toxicity. Real-time polymerase chain reaction (PCR) monitoring of EBV-DNA showed a transient increase in plasma EBV-DNA suggestive of lysis of EBV-infected cells, although there was no consistent decrease in virus load in peripheral-blood mononuclear cells. Interferon-γ enzyme-linked immunospot (ELISPOT) assay and tetramer analysis showed an increase in the frequency of EBV-responsive T cells, which returned to preinfusion levels after 2 to 6 months. None of the treated patients developed PTLD. One patient with liver PTLD showed a complete response, and one with ocular disease has had a partial response stable for over one year. These data are consistent with an expansion and persistence of adoptively transferred EBV-CTLs that is limited in the presence of continued immunosuppression but that nonetheless produces clinically useful antiviral activity.
Streptococcus pneumoniae induced c-Jun-N-terminal kinase- and AP-1 -dependent IL-8 release by lung epithelial BEAS-2B cells
Authors: Schmeck Bernd , Moog Kerstin , Zahlten Janine , van Laak Vincent , N'Guessan Philippe Dje , Opitz Bastian , Rosseau Simone , Suttorp Norbert , Hippenstiel Stefan 
Published: July 2006

AbstractBackgroundAlthough pneumococcal pneumonia is one of the most common causes of death due to infectious diseases, little is known about pneumococci-lung cell interaction. Herein we tested the hypothesis that pneumococci activated pulmonary epithelial cell cytokine release by c-Jun-NH2-terminal kinase (JNK)MethodsHuman bronchial epithelial cells (BEAS-2B) or epithelial HEK293 cells were infected withS. pneumoniaeR6x and cytokine induction was measured by RT-PCR, ELISA and Bioplex assay. JNK-phosphorylation was detected by Western blot and nuclear signaling was assessed by electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP). JNK was modulated by the small molecule inhibitor SP600125 and AP1 by transfection of a dominant negative mutant.ResultsS. pneumoniaeinduced the release of distinct CC and CXC, as well as Th1 and Th2 cytokines and growth factors by human lung epithelial cell line BEAS-2B. Furthermore, pneumococci infection resulted in JNK phosphorylation in BEAS-2B cells. Inhibition of JNK by small molecule inhibitor SP600125 reduced pneumococci-induced IL-8 mRNA expression and release of IL-8 and IL-6. One regulator of theil8promoter is JNK-phosphorylated activator protein 1 (AP-1). We showed thatS. pneumoniaetime-dependently induced DNA binding of AP-1 and its phosphorylated subunit c-Jun with a maximum at 3 to 5 h after infection. Recruitment of Ser63/73-phosphorylated c-Jun and RNA polymerase II to the endogenousil8promoter was found 2 h afterS. pneumoniaeinfection by chromatin immunoprecipitation. AP-1 repressor A-Fos reduced IL-8 release by TLR2-overexpressing HEK293 cells induced by pneumococci but not by TNFα. Antisense-constructs targeting the AP-1 subunits Fra1 and Fra2 had no inhibitory effect on pneumococci-induced IL-8 release.ConclusionS. pneumoniae-induced IL-8 expression by human epithelial BEAS-2B cells depended on activation of JNK and recruitment of phosphorylated c-Jun to theil8promoter.
The claudin gene family: expression in normal and neoplastic tissues
Authors: Hewitt Kyle J , Agarwal Rachana , Morin Patrice J 
Published: July 2006

AbstractBackgroundThe claudin (CLDN) genes encode a family of proteins important in tight junction formation and function. Recently, it has become apparent thatCLDNgene expression is frequently altered in several human cancers. However, the exact patterns ofCLDNexpression in various cancers is unknown, as only a limited number ofCLDNgenes have been investigated in a few tumors.MethodsWe identified all the humanCLDNgenes from Genbank and we used the large public SAGE database to ascertain the gene expression of all 21CLDNin 266 normal and neoplastic tissues. Using real-time RT-PCR, we also surveyed a subset of 13CLDNgenes in 24 normal and 24 neoplastic tissues.ResultsWe show that claudins represent a family of highly related proteins, with claudin-16, and -23 being the most different from the others. Fromin silicoanalysis and RT-PCR data, we find that most claudin genes appear decreased in cancer, whileCLDN3,CLDN4, andCLDN7are elevated in several malignancies such as those originating from the pancreas, bladder, thyroid, fallopian tubes, ovary, stomach, colon, breast, uterus, and the prostate. Interestingly,CLDN5is highly expressed in vascular endothelial cells, providing a possible target for antiangiogenic therapy.CLDN18might represent a biomarker for gastric cancer.ConclusionOur study confirms previously knownCLDNgene expression patterns and identifies new ones, which may have applications in the detection, prognosis and therapy of several human cancers. In particular we identify several malignancies that expressCLDN3andCLDN4. These cancers may represent ideal candidates for a novel therapy being developed based on CPE, a toxin that specifically binds claudin-3 and claudin-4.
Comparison of lentiviral vector titration methods
Authors: Geraerts Martine , Willems Sofie , Baekelandt Veerle , Debyser Zeger , Gijsbers Rik 
Published: July 2006

Abstract

Background
Lentiviral vectors are efficient vehicles for stable gene transfer in dividing and non-dividing cells. Several improvements in vector design to increase biosafety and transgene expression, have led to the approval of these vectors for use in clinical studies. Methods are required to analyze the quality of lentiviral vector production, the efficiency of gene transfer and the extent of therapeutic gene expression.

Results
We compared lentiviral vector titration methods that measure pg p24/ml, RNA equivalents/ml, transducing units (TU/ml) or mRNA equivalents. The amount of genomic RNA in vector particles proves to be reliable to assess the production quality of vectors encoding non-fluorescent proteins. However, the RNA and p24 titers of concentrated vectors are rather poor in predicting transduction efficiency, due to the high variability of vector production based on transient transfection. Moreover, we demonstrate that transgenic mRNA levels correlate well with TU and can be used for functional titration of non-fluorescent transgenes.

Conclusion
The different titration methods have specific advantages and disadvantages. Depending on the experimental set-up one titration method should be preferred over the others.
Purchasing online journal access for a hospital medical library: how to identify value in commercially available products
Authors: Carter Thomas P , Carter Anne O , Broomes Gwendolyn 
Published: July 2006

Abstract
Background
Medical practice today requires evaluating large amounts of information which should be available at all times. This information is found most easily in a digital form. Some information has already been evaluated for validity (evidence based medicine sources) and some is in unevaluated form (paper and online journals). In order to improve access to digital information, the School of Clinical Medicine and Research at the University of the West Indies and Queen Elizabeth Hospital decided to enhance the library by offering online full text medical articles and evidence based medicine sources. The aim of this paper is to evaluate the relative value of online journal commercial products available for a small hospital and medical school library.

Methods
Three reference standards were chosen to represent the ideal list of core periodicals for a broad range of medical care: 2 Brandon/Hill selected lists of journals for the small medical library (BH and BH core) and the academic medical library core journal collection chosen for the Florida State University College of Medicine Medical Library. Six commercially available collections were compared to the reference standards and to the current paper journal subscription list as regards to number of journals matched and cost per journal matched. Ease of use and presence of secondary sources were also considered.

Results
The cost per journal matched ranged from US $ 3194 to $ 81. Because of their low subscription prices, the Biomedical Reference Collection and Proquest products were the most cost beneficial. However, they provided low coverage of the ideal lists (12 – 17% and 21–32% respectively) and contained significant embargoes on current editions, were not user friendly and contained no secondary sources. The Ovid Brandon/Hill Plus Collection overcame these difficulties but had a much higher cost-benefit range while providing higher coverage of the ideal lists (14–47%).

Conclusion
After considering costs, benefits, ease of use, embargoes, presence of secondary sources (ACP Journal Club, DARE), the Ovid Brandon/Hill Plus Collection was the best choice for our hospital considering our budget. However, the option to individually select our own journal list from Ovid and pay per journal has a certain appeal as well.
Attractin, a dipeptidyl peptidase IV/CD26-like enzyme, is expressed on human peripheral blood monocytes and potentially influences monocyte function
Authors: Wrenger Sabine , Faust Jürgen , Friedrich Daniel , Hoffmann Torsten , Hartig Roland , Lendeckel Uwe , Kähne Thilo , Thielitz Anja , Neubert Klaus , Reinhold Dirk 
Published: July 2006

Abstract
The ectoenzyme dipeptidyl peptidase IV (DP IV; CD26) was shown to play a crucial role in T cell activation. Several compounds inhibiting DP IV-like activity are currently under investigation for the treatment of Type 2 diabetes, rheumatoid arthritis, colitis ulcerosa, psoriasis, multiple sclerosis, and other diseases. In the present study, we show that human peripheral blood monocytes express a DP IV-like enzyme activity, which could be inhibited completely by the synthetic DP IV inhibitor Lys[Z(NO2)]-thiazolidide. DP IV immunoreactivity was not detectable on monocytes, and DP IV transcript levels of monocytes were near the detection limit of quantitative polymerase chain reaction. However, monocytes exhibit a strong mRNA expression of the multifunctional DP IV-like ectoenzyme attractin and were highly positive for attractin in flow cytometric analysis. Fluorescence microscopy clearly demonstrated that attractin is located on the cell surface of monocytes. Attractin immunoprecipitates hydrolyzed Gly-Pro-pNA, indicating that monocyte-expressed attractin possesses DP IV-like activity. Inhibitor kinetic studies with purified human plasma attractin revealed that Lys[Z(NO2)]-thiazolidide not only inhibits DP IV but also attractin (50% inhibition concentration=8.45×10−9 M). Studying the influence of this inhibitor on monocyte functions, we observed a clear reduction of cell adhesion to fibronectin-coated culture plates in the presence of Lys[Z(NO2)]-thiazolidide. Moreover, this inhibitor significantly modulates the production of interleukin-1 (IL-1) receptor antagonist, IL-6, and transforming growth factor-β1 in lipopolysaccharide-stimulated monocyte cultures. In summary, here, we demonstrate for the first time expression of attractin on monocytes and provide first data suggesting that drugs directed to DP IV-like enzyme activity could affect monocyte function via attractin inhibition.
Thyrotropin Receptor Autoantibodies Are Independent Risk Factors for Graves’ Ophthalmopathy and Help to Predict Severity and Outcome of the Disease
Authors: Eckstein Anja K. , Plicht Marco , Lax Hildegard , Neuhäuser Markus , Mann Klaus , Lederbogen Sebastian , Heckmann Christian , Esser Joachim , Morgenthaler Nils G. 
Published: July 2006

Abstract
Objective: The objective of this study was to examine whether TSH-receptor antibody [TSH binding inhibitory antibodies (TBII)] levels are associated with the severity of Graves’ ophthalmopathy (GO) over the entire course of the disease.
Methods and Patients: A total of 159 patients with GO were followed for 12–24 months. One year after the first symptoms of GO, all patients were classified into mild or severe GO according to their clinical manifestations. TBII were measured every 3 months after onset of GO. Receiver operating characteristic plot analysis was performed to assess the power to discriminate both patient groups by TBII (specificity &gt;90%).
Results: TBII levels and prevalence at each time point during follow-up were significantly higher in patients with a severe course of GO compared with patients with a mild course of GO. Prognostic statements on the course of the disease were possible for about half of the GO patients at all time points (except the first). If at first presentation and at consecutive time points TBII levels were less than 5.7, 2.6, 1.5, 1.5, 1.5, and 1.5 IU/liter, the patients had a 2.3- to 15.6-fold higher chance of a mild course. If 5–8 months after GO onset and at consecutive time points TBII levels were above 8.8, 5.1, 4.8, 2.8, and 2.8 IU/liter, the patients had a 8.7- to 31.1-fold higher risk of a severe course. This relationship of TBII to the severity was independent from age and smoking.
Conclusion: Follow-up measurements of TBII allow, in half of the patients, assessment of the prognosis of GO and, therefore, could be of additional help for the disease management.
Magnetic Phase Transition of the 1,3-Bis(2,2′-biphenylene)-2-phenylallyl (BDPA) Complex with Acetone
Authors: Yamauchi Jun 
Published: July 2006

Abstract
The magnetic susceptibility of the 1,3-bis(2,2′-biphenylene)-2-phenylallyl (BDPA) complex with acetone exhibited a broad maximum at around 10 K, characterized by a linear Heisenberg-type magnetic interaction; a discontinuity in dχ/dT at ca. 2.8 K was observed, suggesting an antiferromagnetic spin-ordering. The magnetic behavior in the paramagnetic region is discussed in terms of the Weiss temperature, exchange interaction parameters, and a divergent character in the ESR linewidth and g-value. The spin-ordered state was ascertained by antiferromagnetic resonance, in which one of the resonance branches showed a rapid temperature dependence, indicating a transition temperature of 3.1 K. Compared with the magnetic data of the BDPA–benzene complex, it was concluded that the magnetic characteristics were greatly influenced by solvent complexation and that the magnetic parameters in both the paramagnetic and antiferromagnetic regions were enhanced by changing the incorporated solvent from benzene to acetone.
NQR and X-Ray Study of Bis(4-methylpyridinium) Pentabromoantimonate(III), (4-CH3C5H4NH)2SbBr5
Authors: Ishihara Hideta , Dou Shi-qi , Weiss Alarich 
Published: July 2006

Abstract
The crystal structure of the title compound was determined (T = 300 K): , Z = 2, a = 1085.8, b = 1064.3, c = 929.0 pm, α = 97.83, β = 108.86, γ = 99.24°, R(F) = 0.047, Rw(F) = 0.041. Characteristic for the structure is the dimer structure of SbBr52− ion. The atomic coordination around the Sb atom is a distorted octahedron. The 81Br NQR spectrum was studied in the temperature range 77 ≤ T/K ≤ 350. The 81Br NQR frequencies are spread out over a wide range at 77 K: 37 ≤ ν(81Br)/MHz ≤ 111. The temperature dependence of the NQR frequencies and of their spread is given as is an assignment of 81Br NQR lines to the positions of Br atoms in the anion.
Monte-Carlo Simulation Taking Account of Solvent Effects for a Conformation Analysis of Peptides in Solution
Authors: Kuroda Masataka , Yamazaki Kazuto , Kobayashi Tetsu , Fujii Nobutaka , Taga Tooru 
Published: July 2006

Abstract
Solvent effects were introduced into a Monte-Carlo simulation of peptide conformations by the use of a new potential function for evaluating the nonbonding interactions between solute and solvent molecules. The Monte-Carlo program, which included the electrostatic and nonbonding interactions of the solvent, was tested for a conformation analysis of the zwitterionic tetragastrin in DMSO. The theoretical coupling constants were in good agreement with the NMR data. The conformation of the zwitterion was a folded compact form at the lowest energy, which was different from those obtained from a simulation in vacuo and that without nonbonding solvent effects.
Sol–Gel Preparation and Third-Order Nonlinear Optical Properties of TiO2 Thin Films
Authors: Hashimoto Tadanori , Yoko Toshinobu , Sakka Sumio 
Published: July 2006

Abstract
Rutile and anatase thin films have been prepared by the sol–gel method using Ti(OC3H7i)4. Third-order nonlinear optical properties of both TiO2 thin films have been investigated by the third-harmonic generation (THG) method and the effect of the polymorph of TiO2 on the third-order nonlinear optical susceptibility, χ(3), has been examined. The measured χ(3) values of rutile and anatase thin films were 1.4 × 10−12 and 9.7 × 10−13 esu, respectively. The χ(3) values corrected for the porosity of the film were 4.0 × 10−12 (rutile) and 2.4 × 10−12 esu (anatase), which are about 100 times as high as that of SiO2 glass used as standard sample (2.8 × 10−14 esu). The measured and corrected χ(3) values were discussed in comparision with those calculated on the basis of several models.
Synthesis and Characterization of New Style of Water-Soluble Glycosylated Porphyrins as a Spectrophotometric Reagent for Metal Ions
Authors: Kohata Katsunori , Higashio Hisao , Yamaguchi Yuichi , Koketsu Mamoru , Odashima Tsugikatsu 
Published: July 2006

Abstract
Six new styles of water-soluble glycosylated porphyrins (5, 10, 15, 20-tetrakis[2-, 3- or 4-(β-d-glucopyranosyl)phenyl]porphine) were synthesized and studied concerning their structures by 1H and 13C NMR. Tetrakis (o-substituted phenyl)porphine, consisting of four atropisomers (αβαβ, ααββ, αααβ, and αααα), was clearly assigned based on the 13C NMR peak-splitting pattern. It was especially noteworthy that water-soluble picket-fence porphyrin (αααα) was obtained. Furthermore, their characterization as a chromogenic reagent for metal ions was investigated. The introduction of highly water-soluble glucose successfully improved the aggregration and adsorption characteristics of the anionic or cationic porphyrins so far prepared without any marked change in the other important analytical properties.
Theoretical Estimation of the Redox Potentials Involving Organic and Inorganic Free Radicals
Authors: Abe Takehiro 
Published: July 2006

Abstract
A simple theoretical expression for the redox potential of a redox couple (X/X−• or X•/X−) has been presented by using a modified Born equation and has been successfully applied to the organic and inorganic redox couples the reliable standard redox potentials of which are directly measured electrochemically, and to polarographic half-wave redox potentials of organic compounds. The expression has also been applied to inorganic redox couples the provisional standard redox potentials of which are estimated indirectly by using thermodynamic cycles, to examine the validity of the provisional potentials. At least, the provisional redox potentials, with which the potentials calculated approximately agree, seem to be reliable.