bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnosis And Treatment Trends In Pediatric Stone Disease: Preferences of Urologists In The Field
Authors: Akdogan Nebil , Deger Mutlu , Yılmaz Ismail Onder , Ates Tunahan , Arıdogan Ibrahim Atilla , Satar Nihat 
Published: March 2024

Abstract
Introduction:
Many different treatment options exist for pediatric stone disease (PSD). We conducted a survey among urologists in Turkey to find out which diagnostic and therapeutic method urologists choose for stones of different localization and size in pediatric patients of varying age groups.
Materials and Methods
A survey on treatment options in various PSD was developed for urologists working in hospitals of different statuses. The survey consisted of 36 multiple-choice questions, and the average response time was 5 minutes. The measure taken to avoid repetitive responses was that the survey could only be completed once from an internet protocol.
Results
The number of respondents was 95. 91.67%, 89.47%, and 80.21% of the participants preferred ultrasonography as the diagnostic method in the 0–2, 2–6, and 6–18 age ranges, respectively. In treating staghorn kidney stones between 0–2 and 2–6 years, mini percutaneous nephrolithotomy (PCNL) was preferred most frequently, followed by standard PCNL. In all age groups, shockwave lithotripsy was the most common procedure for symptomatic pelvic stones smaller than 10 mm.
Conclusions
Urologists in Turkey act following the guidelines. However, this is not always possible due to lack of facilities. The necessary facilities for urologists need to be improved.
Disentangling the impact of soil type, geographical location, altitude, and family identity on the flower and leaf microbiome in alpine plants
Authors: Abdelfattah Ahmed , Ramakrishnan Dinesh Kumar , Jauernegger Franziska , Hoefle Daniel , Berg Christian , Berg Gabriele 
Published: March 2024

Abstract
Background
While substantial research has explored rhizosphere and phyllosphere microbiomes, knowledge on flower microbiome, particularly in wild plants remains limited. This study explores into the diversity, abundance, and composition of bacterial and fungal communities on leaves and flowers of wild flowering plants in their natural alpine habitat, considering the influence of environmental factors.
Methods
We investigated 50 wild flowering plants representing 22 families across seven locations in Austria. Sampling sites encompassed varied soil types (carbonate/silicate) and altitudes (450–2760 m). Amplicon sequencing to characterize bacterial and fungal communities and quantitative PCR to assess microbial abundance was applied, and the influence of biotic and abiotic factors assessed.
Results
Our study revealed distinct bacterial and fungal communities on leaves and flowers, with higher diversity and richness on leaves (228 fungal and 91 bacterial ASVs) than on flowers (163 fungal and 55 bacterial ASVs). In addition, Gammaproteobacteria on flowers and Alphaproteobacteria on leaves suggests niche specialization for plant compartments. Location significantly shaped both community composition and fungal diversity on both plant parts. Notably, soil type influenced community composition but not diversity. Altitude was associated with increased fungal species diversity on leaves and flowers. Furthermore, significant effects of plant family identity emerged within a subset of seven families, impacting bacterial and fungal abundance, fungal Shannon diversity, and bacterial species richness, particularly on flowers.
Conclusion
This study provides novel insights into the specific microbiome of wild flowering plants, highlighting adaptations to local environments and plant-microbe coevolution. The observed specificity indicates a potential role in plant health and resilience, which is crucial for predicting how microbiomes respond to changing environments, ultimately aiding in the conservation of natural ecosystems facing climate change pressures.
A novel monomer of Elephantopus mollis H.B.K., EM-6, inhibits the proliferation of Huh-7 cells by blocking autophagic flux and activating the ROS/MAPK pathway
Authors: Hou Changyan , Lou Ruoxuan , Wu Zhihui , Tang Jingjing , Zhao Na , Xu Xinwen , Zhou Junzhen , Xiong Peiqian , Li Qiang , Zhang Qing , Jiang Yue , Jiang Jianwei 
Published: March 2024

Abstract
Purpose
Primary liver disease is one of the major health problems in the world, and the prognosis of liver cancer is very poor. Liver cancer cells develop strong resistance to clinical chemotherapy drugs, leading to repeated liver cancer.
Materials and Methods
RNA-sequence was applied to related signal pathways that significantly altered. Flow cytometry and Western blot were performed to detect the changes of cell cycle, apoptosis and MAPK pathways. Immunofluorescence and flow cytometry were used to detect changes in cell mitochondrial membrane potential and intracellular ROS levels. Western blot, immunofluorescence, qRT-PCR and mCherry-GFP-LC3 were used to detect the effect of autophagy. Western blot and qRT-PCR were utilized to detect the effect of ER Stress.
Results
EM-6 is a novel monomer purified from Elephantopus mollis H.B.K. Mechanistically, compared with cisplatin (CDDP), EM-6 significantly inhibited the proliferation of human hepatocellular cancer cell lines and had less toxicity to human normal epithelial cells. EM-6 can induce mitochondrial membrane potential disruption, which leads to the accumulation of ROS, S-phase arrest and activation of the IRE1α-ASK1-JNK/p38 pathway to promote apoptosis in Huh-7 cells. In addition, EM-6 blocked protective autophagy by inhibiting the initiation of autophagy, and inhibiting the formation of autophagolysosomes triggered Huh-7 cell apoptosis.
Conclusion
Taken together, our findings suggest that EM-6 activates mitochondrial apoptosis through the ROS/MAPK pathway and promotes the activation of ER stress and the inhibition of autophagic flux to exacerbate apoptosis. These studies demonstrated the promising future of EM-6 in the clinical treatment of hepatocellular cancer.
Deciphering Abnormal Platelet Subpopulations in Inflammatory Diseases through Machine Learning and Single-Cell Transcriptomics
Authors: Qiu Xinru , Nair Meera G. , Jaroszewski Lukasz , Godzik Adam 
Published: March 2024

Abstract

The study focuses on understanding the transcriptional heterogeneity of activated platelets and its impact on diseases like sepsis, COVID-19, and systemic lupus erythematosus (SLE). Recognizing the limited knowledge in this area, our research aims to dissect the complex transcriptional profiles of activated platelets to aid in developing targeted therapies for abnormal and pathogenic platelet subtypes. We analyzed single-cell transcriptional profiles from 47,977 platelets derived from 413 samples of patients with these diseases, utilizing Deep Neural Network (DNN) and eXtreme Gradient Boosting (XGB) to distinguish transcriptomic signatures predictive of fatal or survival outcomes. Our approach included source data annotations and platelet markers, along with SingleR and Seurat for comprehensive profiling. Additionally, we employed Uniform Manifold Approximation and Projection (UMAP) for effective dimensionality reduction and visualization, aiding in the identification of various platelet subtypes and their relation to disease severity and patient outcomes. Our results highlighted distinct platelet subpopulations that correlate with disease severity, revealing that changes in platelet transcription patterns can intensify endotheliopathy, increasing the risk of coagulation in fatal cases. Moreover, these changes also seem to impact lymphocyte function, indicating a more extensive role for platelets in inflammatory and immune responses. This study sheds light on the crucial role of platelet heterogeneity in serious health conditions, paving the way for innovative therapeutic approaches targeting platelet activation, which could potentially improve patient outcomes in diseases characterized by altered platelet function.
PFC/M1 activation and excitability: a longitudinal cohort study on fatigue symptoms in healthcare workers post-COVID-19
Authors: Han Tao , Dai Chunqiu , Liang Ying , Lin Xiaodong , Gao Ming , Liu Xinyu , Wu Xiangbo , Lu Yuheng , Xi Xiao , Tian Fei , Zhao Chenguang , Sun Xiaolong , Yuan Hua 
Published: March 2024

Abstract

Background
Fatigue is one of the most common neurological symptoms reported post coronavirus disease 2019 (COVID-19) infection. In order to establish effective early intervention strategies, more emphasis should be placed on the correlation between fatigue and cortical neurophysiological changes, especially in healthcare workers, who are at a heightened risk of COVID-19 infection.
Methods
A prospective cohort study was conducted involving 29 COVID-19 medical workers and 24 healthy controls. The assessment included fatigue, sleep and health quality, psychological status, and physical capacity. Functional near-infrared spectroscopy (fNIRS) was employed to detect activation of brain regions. Bilateral primary motor cortex (M1) excitabilities were measured using single- and paired-pulse transcranial magnetic stimulation. Outcomes were assessed at 1, 3, and 6 months into the disease course.
Results
At 1-month post-COVID-19 infection, 37.9% of patients experienced severe fatigue symptoms, dropping to 10.3% at 3 months. Interestingly, the remarkable decreased activation/excitability of bilateral prefrontal lobe (PFC) and M1 were closely linked to fatigue symptoms after COVID-19. Notably, greater increase in M1 region excitability correlated with more significant fatigue improvement. Re-infected patients exhibited lower levels of brain activation and excitability compared to single-infection patients.
Conclusions
Both single infection and reinfection of COVID-19 lead to decreased activation and excitability of the PFC and M1. The degree of excitability improvement in the M1 region correlates with a greater recovery in fatigue. Based on these findings, targeted interventions to enhance and regulate the excitability of M1 may represent a novel strategy for COVID-19 early rehabilitation.
Trial registration
The Ethics Review Committee of Xijing Hospital, No. KY20232051-F-1, registered February 3, 2023. The Chinese Clinical Trial Registry, ChiCTR2300068444, registered February 20, 2023. https://www.chictr.org.cn
The Correlation Between the Movement, Legislation, Paradigm, and Existence of Cooperatives Plays a Crucial Role in Achieving Success in Providing Services.
Authors: Samad Abdul Wahab , Sandopart Dewa Putu Yohanes Agata L. 
Published: March 2024

Abstract
The development and expansion of cooperatives in Indonesia, which serve as a fundamental pillar of the economy, have been subject to historical fluctuations within the country. During the New Order era, there was significant progress in assisting farmers through the establishment of Village Unit Cooperatives (Koperasi Unit Desa, KUD). However, these cooperatives are today facing obstacles and setbacks. To assess the difficulties and advantages of cooperatives, it is necessary to establish a cooperative framework that encompasses the cooperative movement and the support of regulatory norms. This paradigm ensures the continued presence and provision of cooperative products in the economy. The research was conducted utilizing quantitative methodologies, specifically utilizing smart Partial Least Square (Smart PLS) analysis. A study was undertaken to investigate the scope of opportunities and challenges for the cooperative market to integrate into the Indonesian economy. The findings, from the data discovered by the academic community at the Institute of Business and Informatics in 1957, indicate that the p value is less than 0.05, indicating a significant correlation with a value over 0.7. Therefore, there is a significant potential for the development and establishment of cooperatives in Indonesia, which can serve as a fundamental pillar of the country's thriving economy in the future.
Faktor – Faktor yang Berhubungan dengan Anemia pada Ibu Menyusui di Daerah Pertanian
Authors: Hayati Nur , Pangestuti Dina Rahayuning , Pradigdo Siti Fatimah 
Published: March 2024

This study aims to analyze the factors associated with anemia in breastfeeding mothers in agricultural areas. This type of research used analytic observational with a cross sectional approach. The sample of this study was 50 breastfeeding mothers who met the inclusion criteria. The research instrument consisted of informed consent, Questionnaire, Semi Quantitative-Food Frequency Questionnaire (SQ-FFQ), easy touch GCHb, nutrition application and data processing software. The statistical test used was the Chi-Square test. The results showed that the proportion of anemia in breastfeeding mothers was 50%. The results of the Chi-Square test showed that there was no significant relationship between hydration, micronutrient intake, exposure to cigarette smoke, tea intake, and physical activity with anemia in breastfeeding mothers (p0.05). However, there was a relationship between family income and anemia in breastfeeding mothers (p=0.004, p0.05). Factors related to the incidence of anemia in breastfeeding mothers in agricultural areas are family income. The community is expected to be able to control the factors that can affect anemia in breastfeeding mothers. Tujuan penelitian adalah untuk menganalisis faktor–faktor yang berhubungan dengan anemia ibu menyusui di daerah pertanian. Jenis penelitian yang digunakan observasional analitik dengan pendekatan cross sectional. Sampel penelitian ini adalah 50 ibu menyusui yang memenuhi kriteria inklusi. Instrumen penelitian terdiri dari inform consent, kuesioner recall aktivitas fisik, form Semi Quantitative-Food Frequency Questionaire (SQ-FFQ), easy touch GCHb, aplikasi nutrisurvey, dan perangkat lunak pengolah data. Uji statistik yang digunakan uji Chi-Square. Hasil penelitian menunjukkan bahwa proporsi anemia pada ibu menyusui sebesar 50%. Hasil Uji Chi-Square menunjukkan tidak terdapat hubungan yang bermakna antara hidrasi, asupan mikronutrien, paparan asap rokok, asupan teh, dan aktivitas fisik dengan anemia pada ibu menyusui (p0,05). Namun, terdapat hubungan antara pendapatan keluarga dengan anemia ibu menyusui (p=0,004, p0,05). Faktor yang berhubungan dengan kejadian anemia ibu menyusui di daerah pertanian adalah pendapatan keluarga. Bagi masyarakat diharapkan dapat melakukan pengendalian faktor – faktor yang dapat memengaruhi anemia pada ibu menyusui.
Pengaruh Open-Ended terhadap Prestasi Belajar, Berpikir Kritis dan Kepercayaan Diri Siswa SMP
Authors: Koriyah Vivi Nur , Harta Idris 
Published: March 2024

Tujuan penelitian ini adalah untuk mendeskripsikan pengaruh pendekatan open-ended, pengaruh sekolah dan interaksi antara pendekatan open-ended dengan kategori sekolah terhadap prestasi belajar matematika, kemampuan berpikir kritis dan kepercayaan diri siswa SMP. Penelitian ini menggunakan penelitian eksperimen semu yang populasinya mencakup seluruh siswa kelas VII SMPN di Kecamatan Kebumen yang dikelompokkaan menjadi sekolah standar nasional (SSN) dan sekolah potensial. Sampel terdiri dari empat kelas yang dipilih secara acak dari masing-masing kategori sekolah. Teknik pengumpulan data dalam penelitian ini adalah tes dan nontes, sedangkan instrumen yang digunakan untuk mengumpulkan data meliputi soal tes prestasi belajar matematika, soal tes kemampuan berpikir kritis, dan angket kepercayaan diri siswa. Data yang dianalisis menggunakan uji statistik Analisis Varians Multivariat Dua Jalur (Two Way MANOVA) adalah data gain (gain score). Hasil penelitian pada taraf signifikan 5% menunjukkan bahwa ada pengaruh pendekatan open-ended terhadap prestasi belajar matematika, kemampuan berpikir kritis, dan kepercayaan diri siswa SMPN di Kecamatan Kebumen, tidak terdapat pengaruh sekolah serta tidak terdapat interaksi antara pendekatan open-ended dan sekolah terhadap prestasi belajar matematika, kemampuan berpikir kritis, dan kepercayaan diri siswa SMP.Kata Kunci: open-ended, prestasi belajar, kemampuan berpikir kritis, kepercayaan diri The Effect of Open-Ended on the Achievement, Critical Thinking and Self-Confidence of Junior High School Students AbstractThe purpose of this study was to describe the effect of the open-ended approach, the effect of school, interaction of open-ended approach and the school categories on mathematics achievement, critical thinking and self-confidence in mathematic of public junior high school students. This study used quasi-experimental research that includes the entire population of students of class VIIth graders in junior high schools in District of Kebumen, classified into a standard national school (SSN) and potential school. The sample consists of four classes chosen at random from each category of the school. Data collection techniques in this research was a test and non-test while the instruments used to collect data covering mathematics,achievement test, critical thinking test, and questionnaire of the confidence of students. The data of gain score were analyzed using Multivariate Statistical analysis of variance test of two lines (Two Way MANOVA). The results of the study, using significance level 5%, showed that there was an effect of open-ended approach on mathematics achievement, critical thinking, and self-confidence of the students of public junior school in Kebumen District, there is no effect of school and there is no interaction of open-ended approach and schools on mathematics achievement, critical thinking and self confidence of the students of public junior high school. Keywords: open-ended, achievement, critical thinking, self-confidence
Pengembangan model asesmen pembelajaran matematika SMA berdasarkan kurikulum 2013
Authors: Mardhiyana Dewi , Jailani Jailani 
Published: March 2024

Tujuan penelitian ini adalah menghasilkan model asesmen pembelajaran matematika SMA yang sesuai dengan Kurikulum 2013 yang layak, yaitu valid, reliabel, dan praktis. Model asesmen yang dikembangkan terdiri dari instrumen asesmen untuk mengukur Kompetensi Inti 1 (KI-1), Kompetensi Inti 2 (KI-2), Kompetensi Inti 3 (KI-3), dan Kompetensi Inti 4 (KI-4). Model pengembangan yang diterapkan adalah model Plomp, yang terdiri dari tahap penelitian awal, pengembangan, dan penilaian. Analisis kevalidan dilakukan dengan menggunakan indeks Aiken. Reliabilitas dilakukan dengan rumus Alpha dan KR-20. Analisis kepraktisan dilakukan dengan analisis deskriptif. Berdasarkan hasil validasi, model asesmen yang dikembangkan memenuhi kriteria valid untuk masing-masing angket, lembar observasi, dan tes. Hasil uji coba menunjukkan bahwa model asesmen yang dikembangkan memenuhi kriteria reliabel dan praktis. Model asesmen mencapai kriteria reliabel berdasarkan hasil tes dan angket. Model asesmen juga mencapai kriteria praktis untuk penilaian guru dan kriteria baik untuk penilaian siswa.Kata Kunci: pengembangan, model asesmen, pembelajaran matematika, Kurikulum 2013 Developing High School Mathematics Learning Assessment Model Based on Curriculum 2013 AbstractThe purpose of this research is to produce a model of high school mathematics learning assessment which is appropriate with Curriculum 2013, is valid, reliable, and practical. The assessment models developed consists of an assessment instrument to measure Core Competencies 1 (KI-1), Core Competence 2 (KI-2), Core Competencies 3 (KI-3), and Core Competence 4 (KI-4). The developing model in this research was Plomp model, which consists of preliminary research, development, dan assesment phase. Validity analysis was performed by using the index Aiken. Reliability test was performed with the formula Alpha and KR-20. The analysis at practicality was with descriptive analysis. The results of the validation show that the assessment models developed are valid based on the questionnaire, observation sheet and test. The results of the tryout show that assessment models are reliable and practical. The assessment models is reliable criteria based on the tests and questionnaires. The assessment model also is practical criteria based on the teacher’s assessment and good criteria for student’s assessment.
Pengembangan Perangkat Pembelajaran untuk Meningkatkan HOTS pada Kompetensi Bangun Ruang Sisi Datar
Authors: Riadi Arifin , Retnawati Heri 
Published: March 2024

Penelitian ini bertujuan untuk menghasilkan perangkat pembelajaran bangun ruang sisi datar untuk meningkatkan higher-order thinking skills (HOTS) menggunakan problem-based learning (PBL) yang meliputi silabus, rencana pelaksanaan pembelajaran (RPP), dan lembar kegiatan siswa (LKS) yang valid, praktis, dan efektif, serta instrumen evaluasi berupa tes yang valid dan reliabel. Jenis penelitian ini adalah penelitian pengembangan. Penelitian ini dilakukan dengan menggunakan model pengembangan Borg Gall (1983) yang dimodifikasi menjadi tiga tahap pengembangan yaitu studi pendahuluan, desain produk, dan tahap pengembangan dan evaluasi. Aspek kevalidan perangkat pembelajaran ditinjau dari penilaian para ahli dan mencapai kategori valid. Aspek kepraktisan mencapai kategori sangat praktis ditinjau dari observasi keterlaksanaan pembelajaran, penilaian guru, dan penilaian siswa. Aspek keefektifan telah tercapai ditinjau dari HOTS siswa. Hasil uji coba lapangan menunjukkan bahwa HOTS siswa mencapai kategori A di SMP Negeri 1 Daha Utara dan kategori B di SMP Negeri 2 Daha Utara.Kata kunci: pengembangan, higher-order thinking skills, problem-based learning Developing Learning Kit to Improve HOTS for Flat Side of Space Competence AbstractThis research was aimed to develop a learning kit for flat side of space competence to improve higher-order thinking skills using problem-based learning, consisting of valid, practical, and effective syllabuses, lesson plans, worksheets, and the valid and reliable of evaluation instrument i.e. test. The research is a development research, which was carried out by the implementation of Borg Gall (1983) development model, which modified into three stages: preliminary study, product design, and development and evaluation. The result of validation by expert jugdement shows that the developed learning kit is feasible to use with valid category. The aspects of practicality was falls into practical category based on observation of learning implementation, and teacher’s and student’s assessment. The aspect of effectiveness could be reached from the student’s HOTS. The result of field study shows that student’s HOTS category gets A at SMP Negeri 1 Daha Utara and B at SMP Negeri 2 Daha Utara. Keywords: development, higher-order thinking skills, problem-based learning