bluuebunny/crossref_metadata_2025_split huggingface dataset:
An integrative genomic approach reveals coordinated expression of intronic miR-335, miR-342, and miR-561 with deregulated host genes in multiple myeloma
Authors: Ronchetti Domenica , Lionetti Marta , Mosca Laura , Agnelli Luca , Andronache Adrian , Fabris Sonia , Deliliers Giorgio Lambertenghi , Neri Antonino 
Published: August 2008

Abstract

Background
The role of microRNAs (miRNAs) in multiple myeloma (MM) has yet to be fully elucidated. To identify miRNAs that are potentially deregulated in MM, we investigated those mapping within transcription units, based on evidence that intronic miRNAs are frequently coexpressed with their host genes. To this end, we monitored host transcript expression values in a panel of 20 human MM cell lines (HMCLs) and focused on transcripts whose expression varied significantly across the dataset.

Methods
miRNA expression was quantified by Quantitative Real-Time PCR. Gene expression and genome profiling data were generated on Affymetrix oligonucleotide microarrays. Significant Analysis of Microarrays algorithm was used to investigate differentially expressed transcripts. Conventional statistics were used to test correlations for significance. Public libraries were queried to predict putative miRNA targets.

Results
We identified transcripts specific to six miRNA host genes (CCPG1, GULP1, EVL, TACSTD1, MEST, and TNIK) whose average changes in expression varied at least 2-fold from the mean of the examined dataset. We evaluated the expression levels of the corresponding intronic miRNAs and identified a significant correlation between the expression levels of MEST, EVL, and GULP1 and those of the corresponding miRNAs miR-335, miR-342-3p, and miR-561, respectively. Genome-wide profiling of the 20 HMCLs indicated that the increased expression of the three host genes and their corresponding intronic miRNAs was not correlated with local copy number variations. Notably, miRNAs and their host genes were overexpressed in a fraction of primary tumors with respect to normal plasma cells; however, this finding was not correlated with known molecular myeloma groups. The predicted putative miRNA targets and the transcriptional profiles associated with the primary tumors suggest that MEST/miR-335 and EVL/miR-342-3p may play a role in plasma cell homing and/or interactions with the bone marrow microenvironment.

Conclusion
Our data support the idea that intronic miRNAs and their host genes are regulated dependently, and may contribute to the understanding of their biological roles in cancer. To our knowledge, this is the first evidence of deregulated miRNA expression in MM, providing insights that may lead to the identification of new biomarkers and altered molecular pathways of the disease.
Prevalence and correlates of foot pain in a population‐based study: the North West Adelaide health study
Authors: Hill Catherine L , Gill Tiffany K , Menz Hylton B , Taylor Anne W 
Published: August 2008

AbstractBackgroundFew population‐based studies have examined the prevalence of foot pain in the general community. The aims of this study were therefore to determine the prevalence, correlates and impact of foot pain in a population‐based sample of people aged 18 years and over living in the northwest region of Adelaide, South Australia.MethodsThe North West Adelaide Health Study is a representative longitudinal cohort study of n = 4,060 people randomly selected and recruited by telephone interview. The second stage of data collection on this cohort was undertaken between mid 2004 and early 2006. In this phase, information regarding the prevalence of musculoskeletal conditions was included. Overall, n = 3,206 participants returned to the clinic during the second visit, and as part of the assessment were asked to report whether they had pain, aching or stiffness on most days in either of their feet. Data were also collected on body mass index (BMI); major medical conditions; other joint symptoms and health‐related quality of life (the Medical Outcomes Study Short Form 36 [SF‐36]).ResultsOverall, 17.4% (95% confidence interval 16.2 – 18.8) of participants indicated that they had foot pain, aching or stiffness in either of their feet. Females, those aged 50 years and over, classified as obese and who reported knee, hip and back pain were all significantly more likely to report foot pain. Respondents with foot pain scored lower on all domains of the SF‐36 after adjustment for age, sex and BMI.ConclusionFoot pain affects nearly one in five of people in the community, is associated with increased age, female sex, obesity and pain in other body regions, and has a significant detrimental impact on health‐related quality of life.
As the Years Go Rolling By
Authors: Edwards Griffith 
Published: August 2008

Research which tries to understand human experience within the time dimension is entering difficult and uncomfortable territory. Long before the first prospecting research worker arrived on this scene with his questionnaires and statistical methods, creative writers had been exploring the same terrain. Take poetry, for instance. When poets write about the passage of time the visions which they conjure seem nearly always to carry menace. An image offered by Yeats exactly captures the sense of helplessness and haplessness which we may all sometimes experience under the pressure of passing time:The years like great black oxen tread the world.And God the herdsman goads them on behind.And I am broken by their passing feet.
Treatment of Depression
Authors: Paykel E. S. 
Published: August 2008

My aim in this lecture is to review a wide range of published studies on the treatment of depression. I want to look at these with one major clinical question in mind: given the range of treatments available, which treatment should be chosen for which patient? In July 1985 a report, published as a special supplement to the American Journal of Psychiatry, called for more research on this topic:“As the number and diversity of the effective drugs and psychosocial interventions expand, clinicians will increasingly want objective, reliable criteria with which to match the patient to the optimal treatment modality and to monitor the response.” (Institute of Medicine, 1985)
Propylene Glycol-Induced Lactic Acidosis in a Patient Receiving Continuous Infusion Pentobarbital
Authors: Miller Melissa A , Forni Allison , Yogaratnam Dinesh 
Published: August 2008

Objective: To report a case of probable propylene glycol (PG) toxicity in a patient receiving continuous infusion of pentobarbital for refractory status epilepticus. Case Summary: A 59-yoar-old woman with a declining mental status was admitted to the intensive care unit for management of status epilepticus. After failing to achieve the therapeutic endpoint of electroencephalogram burst suppression with a continuous infusion of propofol, the sedative regimen was changed to continuous infusion of pentobarbital. The patient received a loading dose of 450 mg (5 mg/kg), and the maintenance infusion was titrated to a dose of 10 mg/kg/h to achieve burst suppression. Twelve hours after the pentobarbital infusion was started, the patient developed an anion gap metabolic acidosis, elevated serum lactate level, hyperosmolality, and increased osmolal gap. The pentobarbital infusion was discontinued, and the patient's acidosis and hyperosmolality resolved. Discussion: Pentobarbital contains 40% v/v of PG, which was thought to be a potential source of the patient's metabolic derangements. Reports of toxicity with drugs containing PG. particularly intravenous lorazepam, have been well described in the literature. What we describe, however, is one of few reports involving intravenous pentobarbital. The Naranjo probability scale supports a probable drug-related adverse event in our patient. Conclusions: PG toxicity is a potential complication associated with intravenouspentobarbital. Practitioners should be aware of the PG content of pentobarbital and should be familiar with the signs and symptoms associated with PG toxicity.
Delayed-Onset Neutropenia with Divalproex Sodium
Authors: Stoner Steven C , Deal Eli , Lurk Jason T 
Published: August 2008

Objective: To report the development of neutropenia in a patient after almost 8 years of being stabilized on delayed-release divalproex sodium (DVPX). Case Summary: A 45-year-old man had been maintained on DVPX for nearly 8 years, with serum valproic acid concentrations of 85-120 mg/L and normal white blood cell (WBC) counts and absolute neutrophil counts (ANCs). Five months prior to the development of neutropenia (defined as ANC <1800 cells/μL), the patient's DVPX dosage was decreased by 250 mg to 1250 mg every morning and 1500 mg every evening. After 2 months of that regimen, the DVPX dosage was increased back to 1500 mg twice daily. Three months alter that increase, the patient's WBC count dropped to 3.7 × 103/μL and ANC was 1199 cells/μL Although the ANC was below 1800 cells/μL, he showed no physical manifestations consistent with neutropenia. DVPX was discontinued, and 2 weeks later the patient's WBC count was 7.2 x 103/μL and ANC was 2290 cells/μL. Discussion: Although a complete blood cell count with differential is a commonly accepted form of therapeutic drug monitoring with DVPX, the monitoring is considered most necessary to identify dose-retated thrombocytopenia. However, neutropenia has been rarely associated with the use of DVPX and could contribute to the development of different types of infection, including those of a bacterial, viral, or fungal origin. Although neutropenia is generally mild in severity, potentially severe DVPX-associated neutropenia can occur any time during the course of therapy, although it is most common within the first few months of treatment. In this case, DVPX was the probable cause of the neutropenia, according to the Naranjo probability scale. However, this case of neutropenia is atypical with respect to the timeframe in which it developed and was identified. Although the documented laboratory findings suggest neutropenia, the patient did not experience any clinical complications as a result. The late onset of the patient's neutropenia is unlike other cases that have been documented in the literature. Conclusions: Hematologic therapeutic drug monitoring continues to be clinically important regardless of whether the patient is early in therapy or even years later in the course. In this patient, continued regular therapeutic drug monitoring identified a suspected drug-related complication and the medication was able to be discontinued without the development of clinical complications.
Towards novel wood-based materials: Chemical bonds between lignin-like model molecules and poly(furfuryl alcohol) studied by NMR
Authors: Nordstierna Lars , Lande Stig , Westin Mats , Karlsson Olov , Furó István 
Published: August 2008

Abstract
Wood modification with furfuryl alcohol is a non-toxic alternative to conventional preservation treatments. A process in which furfuryl alcohol polymerises in situ was previously proposed for chemical modification of wood. In the present work, liquid model systems were investigated using compounds that resemble repeating units of lignin to verify whether chemical bonds form between the furfuryl alcohol polymer and wood. Using different NMR spectroscopic techniques we confirmed that these model compounds do form covalent bonds with the polymerising polymer. The results indicate that the furan polymer grafts to lignin, supporting observations in similar studies performed with genuine wood materials.
The capability of plasma osteoprotegerin as a predictor of cardiovascular disease: a systematic literature review
Authors: Nybo Mads , Rasmussen Lars M 
Published: August 2008

ObjectiveOsteoprotegerin (OPG) strongly inhibits bone resorption and may also serve as a vascular calcification inhibitor. However, recent studies have indicated that high plasma OPG is a strong predictor of cardiovascular disease (CVD) and mortality. To evaluate this capability, the data concerning OPG as a CVD predictor was gathered through a systematic literature review.Design and methodsStudies investigating OPG as a predictor of CVD or mortality were extracted from Medline and the Cochrane Library, retrieving 187 articles. Non-relevant articles were excluded, resulting in a total of 45 articles. After thorough evaluation of the abstracts, only eight prospective studies containing a follow-up period with a clinical emphasis on CVD were eligible for the literature review.ResultsAll studies except one confirmed that OPG measurement adds important prognostic information to the existing markers of CVD and mortality in high-risk populations. Hazard ratios emphasized the significant correlation between plasma OPG concentration and mortality. Due to methodological problems (e.g., population investigated, measurement principle, and statistics performed), meta-analysis could not be performed. As only one study was conducted in a healthy cohort, the results cannot per se be extrapolated to the general population.ConclusionThe combined results support plasma OPG as an independent predictor of CVD and mortality in high-risk populations. However, more longitudinal studies in general cohorts are needed before the use of plasma OPG can be evaluated in this regard.
Acetate-fed aerobic granular sludge for the degradation of chlorinated phenols
Authors: Carucci A. , Milia S. , De Gioannis G. , Piredda M. 
Published: August 2008

In this study, the possibility to use acetate-fed aerobic granular sludge for the degradation of low chlorinated 4-mono-chlorophenol (4CP) and highly chlorinated 2,4,6-tri- chlorophenol (TCP) was investigated. A Granulated Sequencing Batch Reactor (GSBR) was used to carry out the experiments, with acetate as growth substrate. 4CP concentration in the influent ranged between 0 and 50 mg/l, while TCP concentration varied between 0 and 15 mg/l. Different operating conditions were applied in order to obtain the complete aerobic degradation of 4CP. For TCP degradation, anaerobic feeding and control of dissolved oxygen concentration in the bulk liquid were used to keep the granules core under anaerobic conditions due to diffusion limitations: the possibility to obtain TCP reductive dechlorination under aerated conditions was thus investigated. Differences in granules shape and size were observed with 4CP and TCP dosed in the influent, and the effects of such toxic compounds on acetate removal were evaluated.
Aerobic granules grown on acetate as carbon source proved to be an interesting solution for the degradation of 4CP, showing good resistance to high 4CP concentrations in the influent even if unacclimated. The presence of TCP did not irreversibly inhibit biomass activity, and complete TCP degradation was achieved after acclimation.
Pregnancy Insulin, Glucose, and BMI Contribute to Birth Outcomes in Nondiabetic Mothers
Authors: Ong Ken K. , Diderholm Barbro , Salzano Giuseppina , Wingate Dianne , Hughes Ieuan A. , MacDougall Jane , Acerini Carlo L. , Dunger David B. 
Published: August 2008

OBJECTIVE—We investigated the effects of normal variations in maternal glycemia on birth size and other birth outcomes.RESEARCH DESIGN AND METHODS—Women in two unselected birth cohorts, one retrospective (n = 3,158) and one prospective (n = 668), underwent an oral glucose challenge at 28 weeks of gestation. In the retrospective study, glycemia was linked to routine birth records. In the prospective study, offspring adiposity was assessed by skinfold thickness from birth to age 24 months.RESULTS—In the retrospective study, within the nondiabetic range (2.1–7.8 mmol/l), each 1 mmol/l rise in the mother's 60-min glucose level was associated with a (mean ± SEM) 2.1 ± 0.8% (P = 0.006) rise in absolute risk of assisted vaginal delivery, a 3.4 ± 0.8% (P &lt; 0.0001) rise in emergency cesarean delivery, a 3.1 ± 0.7% (P &lt; 0.0001) rise in elective cesarean delivery, and a 46 ± 8 g (P &lt; 0.0001) increase in offspring birth weight. In the prospective study, fetal macrosomia (birth weight &gt;90th centile) was independently related to the mother's fasting glucose (odds ratio 2.61 per +1 mmol/l [95% CI 1.15–5.93]) and prepregnancy BMI (1.10 per +1 kg/m2 [1.04–1.18]). The mother's higher fasting glycemia (P = 0.004), lower insulin sensitivity (P = 0.01), and lower insulin secretion (P = 0.02) were independently related to greater offspring adiposity at birth. During postnatal follow-up, the correlation between the mother's glycemia and offspring adiposity disappeared by 3 months, whereas prepregnancy BMI was associated with offspring adiposity that was only apparent at 12 and 24 months (both P &lt; 0.05).CONCLUSIONS—Prepregnancy BMI, pregnancy glycemia, insulin sensitivity, and insulin secretion all contribute to offspring adiposity and macrosomia and may be separate targets for intervention to optimize birth outcomes and later offspring health.
Candida antarctica Lipase B (CAL‐B)‐Catalyzed Carbon‐Sulfur Bond Addition and Controllable Selectivity in Organic Media
Authors: Lou Feng‐Wen , Liu Bo‐Kai , Wu Qi , Lv De‐Shui , Lin Xian‐Fu 
Published: August 2008

AbstractA novel enzymatic, promiscuous protocol for Candida antarctica lipase B (CAL‐B)‐catalyzed carbon‐sulfur bond addition is described. Some control experiments have been designed to demonstrate the catalytic specificity of CAL‐B. Selectivity between anti‐Markovnikov addition and Markovnikov addition was achieved in different organic media. A series of thioether‐containing ester functional groups was synthesized under the catalysis of CAL‐B at 50 °C. All the products were characterized by spectroscopic methods (IR, NMR, ESI‐MS).
Silica Immobilized Second Generation Hoveyda‐Grubbs: A Convenient, Recyclable and Storageable Heterogeneous Solid Catalyst
Authors: Van Berlo Boris , Houthoofd Kristof , Sels Bert F. , Jacobs Pierre A. 
Published: August 2008

AbstractThe second generation Hoveyda–Grubbs metathesis catalyst was successfully immobilized on silica in pellet and powder form following a practical and fast synthesis procedure. The activity of the solid system is truly heterogeneous, efficient in various metathesis reaction types and stable for at least 4000 TON. Ru contamination of the products was very low (ppb levels). The successful use of the robust system has been demonstrated in a continuous reactor set‐up.
Aerobic Oxidation of Alcohols under Mild Conditions Catalyzed by Novel Polymer‐Incarcerated, Carbon‐Stabilized Gold Nanoclusters
Authors: Lucchesi Céline , Inasaki Takeshi , Miyamura Hiroyuki , Matsubara Ryosuke , Kobayashi Shū 
Published: August 2008

AbstractPolymer‐incarcerated, carbon‐stabilized gold nanoclusters were found to be highly active in heterogeneous catalysis for the oxidation of secondary alcohols using molecular oxygen in aqueous medium. After optimization of catalyst preparation methods, highly loaded and highly effective catalysts were obtained, and a broad range of secondary alcohols could be oxidized by using these catalysts under mild conditions. The catalysts could be recovered and reused several times without significant loss of activity. Moreover, kinetics of the oxidation reaction with (±)‐1‐phenylethanol was investigated.
Ruthenium‐Catalyzed Synthesis of Secondary Alkylamines: Selective Alkylation with Aliphatic Amines
Authors: Bähn Sebastian , Hollmann Dirk , Tillack Annegret , Beller Matthias 
Published: August 2008

AbstractThe chemoselective N‐alkylation of tert‐alkylamines applying aliphatic amines is described for the first time. In the presence of the Shvo catalyst 1, tert‐octylamine 4 and 1‐adamantylamine 5 are alkylated using primary, secondary, and even tertiary amines to give the corresponding monoalkylated tert‐alkylamine in moderate to very good yields and excellent selectivity. This novel reaction proceeds without an additional hydrogen source and ammonia is formed as the only by‐product.
Occupational behaviors and farmworkers' pesticide exposure: Findings from a study in monterey county, California
Authors: Salvatore Alicia L. , Bradman Asa , Castorina Rosemary , Camacho José , López Jesús , Barr Dana B. , Snyder John , Jewell Nicholas P. , Eskenazi Brenda 
Published: August 2008

AbstractBackgroundWe studied the relationship between behaviors promoted through the US Environmental Protection Agency Worker Protection Standard (WPS) and other programs and agricultural pesticide exposures in 73 strawberry fieldworkers employed in Monterey County, California.MethodsFarmworkers' behaviors were assessed via self‐report and organophosphorus (OP) pesticide exposure was measured using dimethyl alkylphosphate (DMAP) and malathion dicarboxylic acid (MDA) urinary metabolite levels.ResultsWearing WPS‐recommended clothing, wearing clean work clothes, and the combination of handwashing with soap and wearing gloves were associated with decreases in DMAP and MDA metabolite levels. Despite these protective behaviors, however, participants had significantly higher levels of exposure as compared with a national reference sample.ConclusionsInterventions that facilitate compliance with these behaviors may be effective in decreasing fieldworkers' pesticide exposures. However, further efforts are needed to reduce the exposure disparities experienced by farmworkers and decrease the potential for “take home” exposures to farmworkers' families. Am. J. Ind. Med. 51:782–794, 2008. Published 2008 Wiley‐Liss, Inc.
Association of cytokine gene polymorphisms in CWP and its severity in Turkish coal workers
Authors: Ates Ilker , Suzen H. Sinan , Yucesoy Berran , Tekin Ishak Ozel , Karakaya Asuman 
Published: August 2008

AbstractBackgroundCytokines appear to play a key role in some inflammatory reactions affecting the interactions among pro‐ and anti‐inflammatory mechanisms that result in several diseases such as coal workers' pneumoconiosis (CWP). In this study, to determine the cytokine gene profiles of Turkish coal miners, we performed genotyping analysis to investigate the polymorphisms of CWP‐related pro‐inflammatory (TNFA, IL1A, IL1B, and IL6) and anti‐inflammatory cytokines (IL‐1RN and TGFB1). An additional goal was to observe whether these cytokine gene polymorphisms influence the development risk and severity of.MethodsGenotyping was carried out by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) technique.ResultsTNFA (−238) gene polymorphism principally affected CWP development and severity (OR = 3.47: 95% CI, 1.12–10.77 and OR = 4.30: 95% CI, 1.25–14.74, respectively) and also risk of CWP (OR = 3.79: 95% CI, 1.37–10.46). The TNFA (−308) variant was associated with a risk for the CWP severity (OR = 2.84: 95% CI, 1.08–7.39). A protective effect of IL6 was found on the development (OR = 0.48: 95% CI, 0.21–0.93) and severity of CWP (OR = 0.37: 95% CI, 0.15–0.91).ConclusionsWe suggest that TNFA (−238) variant may be a risk factor in both development and the severity of CWP, while TNFA (−308) variant seems to be important only in disease severity. On the other hand, IL6 variant may have a protective effect on the development and disease severity. Am. J. Ind. Med. 51:741–747, 2008. Published 2008 Wiley‐Liss, Inc.