bluuebunny/crossref_metadata_2025_split huggingface dataset:
Organelle resolved proteomics uncovers PLA2R1 as a novel cell surface marker required for chordoma growth
Authors: Khan Shahbaz , Zuccato Jeffrey A. , Ignatchenko Vladimir , Singh Olivia , Govindarajan Meinusha , Waas Matthew , Mejia-Guerrero Salvador , Gao Andrew , Zadeh Gelareh , Kislinger Thomas 
Published: March 2024

AbstractChordomas are clinically aggressive tumors with a high rate of disease progression despite maximal therapy. Given the limited therapeutic options available, there remains an urgent need for the development of novel therapies to improve clinical outcomes. Cell surface proteins are attractive therapeutic targets yet are challenging to profile with common methods. Four chordoma cell lines were analyzed by quantitative proteomics using a differential ultracentrifugation organellar fractionation approach. A subtractive proteomics strategy was applied to select proteins that are plasma membrane enriched. Systematic data integration prioritized PLA2R1 (secretory phospholipase A2 receptor–PLA2R1) as a chordoma-enriched surface protein. The expression profile of PLA2R1 was validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates via immunoblotting. PLA2R1 expression was further validated by immunohistochemical analysis in a richly annotated cohort of 25-patient tissues. Immunohistochemistry analysis revealed that elevated expression of PLA2R1 is correlated with poor prognosis. Using siRNA- and CRISPR/Cas9-mediated knockdown of PLA2R1, we demonstrated significant inhibition of 2D, 3D and in vivo chordoma growth. PLA2R1 depletion resulted in cell cycle defects and metabolic rewiring via the MAPK signaling pathway, suggesting that PLA2R1 plays an essential role in chordoma biology. We have characterized the proteome of four chordoma cell lines and uncovered PLA2R1 as a novel cell-surface protein required for chordoma cell survival and association with patient outcome.
Reflective thinking and self-evaluation in language learning: mirroring the impacts on Saudi Arabian EFL students’ growth mindfulness, resilience, and academic well-being
Authors: Hammad Al-Rashidi Anwar , Aberash Amhara 
Published: March 2024

AbstractBased on the related literature, both self-evaluation (SE) and reflective thinking (RT) play an important role in English language learning among EFL learners. Therefore, in this study, the effects of SE and RT on growth mindfulness, resilience, and academic well-being of Saudi Arabian EFL students were compared. Ninety-six intermediate EFL students were selected based on the convenience sampling method in order to accomplish this goal, and they were divided into three groups: two experimental groups (EGs) and one control group (CG). Three questionnaires were then distributed to assess the participants’ academic well-being, progress in mindfulness, and resilience prior to the instruction. After that, the CG was instructed conventionally, while one EG was treated using RT and the other EG was treated utilizing SE activities. The aforementioned surveys were re-administered as study post-tests following a 21-session treatment, and the results were analyzed using One-way ANOVA and Tukey testing. The study’s findings showed that the two EGs conducted better than the CG on three post-tests. Additionally, the outcomes supported the equivalent benefits of RT and SE for the development of Saudi Arabian EFL students’ academic wellbeing, resilience, and mindfulness. The implications were eventually prepared for scholars, English instructors, students, and other stakeholders.
Functional prognosis following spontaneous intracerebral hemorrhage in patients on hemodialysis: a retrospective study of 100 consecutive cases
Authors: Watanabe Yusuke , Suzuki Kaima , Inoue Tsutomu , Kurita Hiroki , Okada Hirokazu 
Published: March 2024

Abstract
Background
Recently, discussions have increasingly focused on “withdrawal from dialysis” among patients undergoing dialysis who experience severe brain dysfunction as post-stroke sequelae. In this context, the lack of understanding regarding functional prognosis after spontaneous intracerebral hemorrhage in patients undergoing hemodialysis becomes a particularly important issue. Therefore, we aimed to evaluate the functional prognosis of intracerebral hemorrhage in patients undergoing hemodialysis when life-saving was prioritized and dialysis was not withdrawn. Furthermore, we sought to identify factors influencing life and functional prognosis.

Methods
We retrospectively analyzed data of 100 consecutive hemodialysis patients who experienced spontaneous intracerebral hemorrhage between 2012 and 2021. The in-hospital mortality rates and modified Rankin Scale (mRS) and Functional Independence Measure (FIM) scores at discharge and 90 days after onset were examined.

Results
The mean age of the patients was 65.7 years, with 29% of them undergoing life-saving brain surgery. The mRS score at discharge was 0, 1, 2, 3, 4, 5, and 6 (indicating death) in 0, 1, 13, 9, 26, 20, and 31 cases, respectively. Among the 69 surviving patients, the median FIM score at discharge was 37 (19–81), with 14 patients having an FIM score of 18 (i.e., complete dependence). Patients who underwent life-saving brain surgery showed a higher median mRS score [5, interquartile range (IQR) 5–6] and a lower FIM score (18, IQR 18–22) at discharge compared with those who did not undergo the surgery. Patients discharged with mRS and FIM scores of 5 and 18, respectively, had the same functional status at 90 days. The Functional Outcome in Patients With Primary Intracerebral Hemorrhage (FUNC) score and intraventricular extension at admission were predictive of a mRS score ≥ 5 (area under the receiver operating characteristic curve = 0.92, p < 0.0001) and FIM score at discharge (adjusted R2 = 0.3, p = 0.0003).

Conclusion
In our single-center study, more than 14% of patients on hemodialysis with cerebral hemorrhage incurred severe, irreversible neurological and functional impairments, necessitating discussions on hemodialysis withdrawal. Moreover, FUNC score and intraventricular extension at admission were highly accurate predictors of functional prognosis.
Revocable and verifiable weighted attribute-based encryption with collaborative access for electronic health record in cloud
Authors: Li Ximing , Wang Hao , Ma Sha , Xiao Meiyan , Huang Qiong 
Published: March 2024

AbstractThe encryption of user data is crucial when employing electronic health record services to guarantee the security of the data stored on cloud servers. Attribute-based encryption (ABE) scheme is considered a powerful encryption technique that offers flexible and fine-grained access control capabilities. Further, the multi-user collaborative access ABE scheme additionally supports users to acquire access authorization through collaborative works. However, the existing multi-user collaborative access ABE schemes do not consider the different weights of collaboration users. Therefore, using these schemes for weighted multi-user collaborative access results in redundant attributes, which inevitably reduces the efficiency of the ABE scheme. This paper proposes a revocable and verifiable weighted attribute-based encryption with collaborative access scheme (RVWABE-CA), which can provide efficient weighted multi-user collaborative access, user revocation, and data integrity verification, as the fundamental cornerstone for establishing a robust framework to facilitate secure sharing of electronic health records in a public cloud environment. In detail, this scheme employs a novel weighted access tree to eliminate redundant attributes, utilizes encryption version information to control user revocation, and establishes Merkle Hash Tree for data integrity verification. We prove that our scheme is resistant against chosen plaintext attack. The experimental results demonstrate that our scheme has significant computational efficiency advantages compared to related works, without increasing storage or communication overhead. Therefore, the RVWABE-CA scheme can provide an efficient and flexible weighted collaborative access control and user revocation mechanism as well as data integrity verification for electronic health record systems.
Physicochemical, in vitro antidiabetic and sensory characteristics of leavened functional bread made with Lasia spinosa and Nelumbo nucifera rhizome flours composited with wheat flour
Authors: Chiranthika N. N. G. , Chandrasekara A. , Gunathilake K. D. P. P. 
Published: March 2024

AbstractBread is considered one of the most popular bakery products consumed in several world regions including Sri Lanka. The potential of utilization of two rhizome flours (Lasia spinosa and Nelumbo nucifera) was evaluated in bread formulation with four different substitution levels (20, 40, 60, and 80%). After the incorporation of L. spinosa and N. nucifera flours, the alterations in compositional, functional, in vitro anti diabetic activity, in vitro starch digestibility and sensory attributes were observed. The results revealed that the total dietary fiber, resistant starch and ash contents were higher while protein, fat and starch contents were lower in the rhizome flour incorporated breads. The functional properties showed that the water absorption index and water solubility increased with increasing of rhizome or proportion. The results showed that 20% rhizome flour incorporated bread had the highest sensory score compared to other percentage ratios and contained a higher level of dietary fiber, resistant starch, antidiabetic activity, and low rate of starch digestibility compared to control bread. The mixture of Lasia spinosa and Nelumbo nucifera rhizome flours could be incorporated up to 20% substitution level with wheat flour to formulate functional bread with more nutritious and functional along with sensory acceptability.
Graphical Abstract
Inhibition of SAT1 alleviates chondrocyte inflammation and ferroptosis by repressing ALOX15 expression and activating the Nrf2 pathway
Authors: Xu Jingting , Ruan Zhaoxuan , Guo Zhou , Hou Liangcai , Wang Genchun , Zheng Zehang , Zhang Xiong , Liu Haigang , Sun Kai , Guo Fengjing 
Published: March 2024

AimsOsteoarthritis (OA) is the most common chronic pathema of human joints. The pathogenesis is complex, involving physiological and mechanical factors. In previous studies, we found that ferroptosis is intimately related to OA, while the role of Sat1 in chondrocyte ferroptosis and OA, as well as the underlying mechanism, remains unclear.MethodsIn this study, interleukin-1β (IL-1β) was used to simulate inflammation and Erastin was used to simulate ferroptosis in vitro. We used small interfering RNA (siRNA) to knock down the spermidine/spermine N1-acetyltransferase 1 (Sat1) and arachidonate 15-lipoxygenase (Alox15), and examined damage-associated events including inflammation, ferroptosis, and oxidative stress of chondrocytes. In addition, a destabilization of the medial meniscus (DMM) mouse model of OA induced by surgery was established to investigate the role of Sat1 inhibition in OA progression.ResultsThe results showed that inhibition of Sat1 expression can reduce inflammation, ferroptosis changes, reactive oxygen species (ROS) level, and lipid-ROS accumulation induced by IL-1β and Erastin. Knockdown of Sat1 promotes nuclear factor-E2-related factor 2 (Nrf2) signalling. Additionally, knockdown Alox15 can alleviate the inflammation-related protein expression induced by IL-1β and ferroptosis-related protein expression induced by Erastin. Furthermore, knockdown Nrf2 can reverse these protein expression alterations. Finally, intra-articular injection of diminazene aceturate (DA), an inhibitor of Sat1, enhanced type II collagen (collagen II) and increased Sat1 and Alox15 expression.ConclusionOur results demonstrate that inhibition of Sat1 could alleviate chondrocyte ferroptosis and inflammation by downregulating Alox15 activating the Nrf2 system, and delaying the progression of OA. These findings suggest that Sat1 provides a new approach for studying and treating OA.Cite this article: Bone Joint Res 2024;13(3):110–123.
Labour pain relief practice by maternal health care providers at a tertiary facility in Kenya: An institution-based descriptive survey
Authors: Ouma Eliazaro Gabriel , Orango Omenge , Were Edwin , Omwodo Kimbley Asaso 
Published: March 2024

Background
Although pain relief is a crucial component of modern obstetric care, it remains a poorly established service in sub-Saharan countries such as Kenya. Maternal health care providers have an extensive role to play in meeting the analgesic needs of women during childbirth. This study sought to examine the practice of labour pain relief among Kenyan maternal health care providers.

Methods
This was an institution-based, cross-sectional, descriptive survey. The study included midwives, obstetricians, and anaesthesiologists (n = 120) working at the second-largest tertiary facility in Kenya. A structured, self-administered questionnaire was used. The labour pain relief practice, knowledge, attitude, and perceived barriers to labour pain management were described.

Results
One hundred and seventeen respondents participated in the study representing a response rate of 97.5%. More than half of maternal health care providers routinely provided the service of labour pain relief (61.5%). Sixty-four (88.9%) respondents reported providing pharmacological and non-pharmacological methods, while 11.1% provided only pharmacological ones. The most common pharmacological method prescribed was non-opioids (12.8%). The most preferred non-pharmacological method of pain management was touch and massage (93.8%). Regional analgesia was provided by 3.4% of the respondents. More than half of the respondents (53%) had poor knowledge of labour pain relief methods. Almost all (94%) of the respondents had a positive attitude towards providing labour pain relief. Non-availability of drugs and equipment (58.1%), lack of clear protocols and guidelines (56.4%), and absence of adequate skilled personnel (55.6%) were reported as the health system factors that hinder the provision of labour analgesia.

Conclusions
More than half of maternal health care providers routinely relieve labour pain. Epidural analgesia is still relatively underutilized. There is a need to develop institutional labour pain management protocols to meet the analgesic needs of women during childbirth.
Non-linear relationship between body roundness index and albuminuria among children and adolescents aged 8–19 years: A cross-sectional study
Authors: Qin Xuankai , Wei Jiahui , Chen Jie , Lei Fengying , Qin Yuanhan 
Published: March 2024

Introduction
Obesity has been found to be correlated with numerous health issues, including an elevated risk of albuminuria in adults. However, this correlation is still controversial among children and adolescents, as several recent large-scale cross-sectional studies have observed a negative correlation between obesity and albuminuria. Our study aimed to investigate the link between the body roundness index (BRI) and albuminuria among children and adolescents, in order to further understand the correlation between obesity and albuminuria in this demographic.

Methods
We employed information from the National Health and Nutrition Examination Survey (NHANES) 1999–2010 for cross-sectional analysis. Weighted logistic regression was employed to explore the linear relationship between BRI and albuminuria, with subgroup analyses performed for more detailed insights. Weighted linear regression analysis was employed to explore the relationship between BRI and the urine albumin-creatinine ratio (UACR). Additionally, we applied smooth curve fitting to investigate their non-linear relationship and conducted threshold effect analysis to identify any turning point.

Results
In this study of 15,487 participants aged 8–19 years, multivariate logistic regression analysis revealed a significant negative correlation between BRI and albuminuria (OR = 0.616, 95%CI: 0.526–0.722). The relationship between BRI and UACR, as shown by multivariate linear regression analysis, was significantly inversely correlated (β: -5.424, 95%CI: -7.416 to -3.433). Furthermore, smooth curve fitting and threshold effect analysis showed a non-linear relationship between BRI and albuminuria, with a BRI inflection point identified at 2.906.

Conclusions
These findings of our study suggest a significant nonlinear negative association between BRI and the presence of albuminuria among children and teenagers, and maintaining an appropriate BRI may decrease the occurrence of albuminuria in this population.
ADMINISTRATIVE TRANSGRESSION AND JUDICIAL DISCRETION FOR THE SAKE OF CITIZENS’ RIGHTS: The Legalisation of Unregistered Marriages in Indonesia
Authors: Fauzi Muhammad Latif 
Published: March 2024

This article addresses how the legalisation of unregistered marriages works in Indonesian day-to-day practices. It understands people’s strategies to legalise their unregistered marriage and examines the state activities of granting legalisation. It seeks to answer in what ways the legal validity of a marriage is important to Muslim citizens. The data in this paper results from the observation of marriage registration documents in a marriage registration office and the examination of legal reasoning used by Islamic judges in marriage legalisation (isbat nikah) cases. This essay emphasizes that state recognition of a marriage cannot entirely substitute community-based legal norms. It also shows the importance of administrative transgression and judicial discretion played by street-level bureaucrats. By so doing, they have grounds to accord legalisation. They apply a lenient approach towards marriage rules that is key to providing state recognition of unregistered marriages.[Abstrak: Tulisan ini membahas bagaimana legalisasi perkawinan tidak tercatat diterapkan dalam praktik sehari-hari di Indonesia. Tulisan ini memahami strategi masyarakat dalam melegalisasi ‘perkawinan tanpa pencatatan’ dan menganalisis alasan negara dalam memberikan legalisasi. Ini untuk menjawab pertanyaan sejauh mana status sah dalam perkawinan penting bagi warga negara Muslim? Data dalam tulisan ini dihasilkan dari pengamatan dokumen pencatatan nikah di kantor pencatatan nikah dan pemeriksaan atas penalaran hukum dalam kasus pengesahan perkawinan (isbat nikah). Tulisan ini menekankan bahwa pengakuan negara atas perkawinan tanpa pencatatan tidak dapat menggantikan norma yang berlaku di masyarakat. Praktik di lapangan menunjukkan pentingnya pelanggaran administratif dan diskresi yudisial yang dimainkan oleh para birokrat tingkat jalanan. Dengan cara demikian, mereka memiliki alasan untuk memberikan legalisasi. Mereka menerapkan pendekatan yang lunak terhadap aturan perkawinan yang merupakan kunci untuk dapat memberikan pengakuan negara atas perkawinan tanpa pencatatan.]
THE DOMINATION OF ISLAMIC LAW IN CUSTOMARY MATRIMONIAL CEREMONIES: Islamic Values within the Malay Marriage Tradition in Kepulauan Riau
Authors: Saiin Asrizal , Umar M. Hasbi , Badarussyamsi Badarussyamsi , Hajazi Moh. Zaidi , Yusuf Maulana 
Published: March 2024

The Malay community has engaged with various traditions, such as those of India, the Middle East, China, and even Europe. Despite that, religion, Islam, has wielded the most significant influence, particularly within the context of marriage life. The process of Muslim Malay marriages consistently adhere to Islamic values, even as these marriage processes are conducted within the framework of local customary practices. This article aims to examine the extent to which these marriage process adopt and synchronize with commonly held Islamic values. Data was gathered through observation and interviews in the Kepulauan Riau. This article reveals that these marriage processes indeed manifest values well-known within Islam. These values constitute in merisik phase symbolizing the principle of at-ta’āruf; menyampaikan hajat symbolizing al-Musyāwarah; menyampaikan belanja being a form of at-ta’āwun; ajak mengajak representing as-syirkah; berbalas pantun signifying al-mau’iẓah; and do'a selamatan embodying as-syukr. These values unequivocally represent the dominance of Islamic Law as the pivotal principles of Customary Law in Kepulauan Riau. [Abstrak: Dalam lintasan sejarah, masyarakat Melayu telah berinteraksi dengan berbagai tradisi, misalnya India, Timur Tengah, China, dan bahkan Eropa. Terlepas dari sisi geografis di atas, Islam memiliki pengaruh yang paling signifikan, terutama dalam prosesi perkawinan. Pada tataran nilai, tahap-tahap pernikahan orang Melayu Muslim selalu menerapkan nilai-nilai Islam meskipun prosesi perkawinan itu dilaksanakan dalam bingkai acara-acara adat setempat. Artikel ini bertujuan untuk melihat sejauh mana tahap-tahap pernikahan itu mengadopsi dan singkron dengan nilai-nilai yang umum dalam Islam. Untuk mendapatkan gambaran yang tepat, kami mengumpulkan data dengan cara observasi dan wawancara di Kepulauan Riau. Artikel ini menemukan bahwa sebenarnya prosesi-prosesi tersebut merupakan manifestasi dari nilai-nilai yang selama ini jamak dikenal dalam Islam. Nilai nilai tersebut antara lain: merisik, menyimbolkan prinsip at-ta’āruf; menyampaikan hajat menyimbolkan al-Musyāwarah; menghantar belanja merupakan bentuk dari at-ta’āwun; ajak mengajak menyimbolkan as-syirkah; berbalas pantun, merupakan al-mau’iẓah; and doa selamatan merupakan as-syukr. Dengan demikian, nilai-nilai tersebut tidak ubahnya merupakan pengejewantahan dari dominasi Hukum Islam sebagai sendi Hukum Adat di Kepulauan Riau.]
Green Competence Building, Green Employee Involvement and Green Work-Life Balance to Improve Environmental Performance through Green Organizational Culture
Authors: PRASETYO Deni Widyo , KUSMANINGTYAS Amiartuti , MUJANAH Siti 
Published: March 2024

This research is motivated by environmental performance. The aim of this study is to determine the influence of the relationship between green competence building, green employee involvement, and green work-life balance on environmental performance, mediated by the green organizational culture of the Head of Study Program at PGRI Higher Education Institutions in East Java. The grand theory of this research is the AMO theory (Ability, Motivation, Opportunity). The sampling technique uses a saturated or census sample. The sample in this study consists of 185 respondents, the Head of Study Program at PGRI Higher Education Institutions in East Java, through a self-administered survey. The research instrument uses a questionnaire and is analyzed using SEM analysis, employing the Warp PLS Version 7.0 analysis tool to analyze the outer model, inner model, and hypothesis testing using p-value. The results show a significant direct influence of green competence building and green employee involvement on green organizational culture. Subsequently, green organizational culture significantly influences environmental performance. The relationship with green work-life balance does not significantly affect green organizational culture.
This research indicates that there is an intervening variable effect, namely green organizational culture, on the relationship between green competence building, green employee involvement, and environmental performance. However, green organizational culture cannot act as an intervening variable in the relationship between green work-life balance and environmental performance. This study demonstrates that the practices of green competence building, green employee involvement, and green organizational culture play a crucial role in the implementation of environmental policies to promote environmental performance at PGRI Higher Education Institutions in East Java.