bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytogenetic Follow‐Up in a Case With a Primary Cutaneous Melanoma and Five Metastatic Lesions
Authors: GRAMMATICO PAOLA , EIBENSCHUTZ LAURA , ROCCELLA FRANCESCA , AMANTEA ADA , ROCCELLA MARIA , CATRICALÀ CATERINA , MICCI FRANCESCA , PORTO GIUSEPPE DEL 
Published: July 2006

Cytogenetic analyses conducted on several cases of melanoma have contributed to the identification of the chromosomal regions where the sequences responsible for malignant transformation and the evolution of this tumor are probably located. With regard to these problems, it is very important to have the possibility to analyze, through the use of cytogenetics, both the primary melanoma and the metastatic lesions from the same patient. We present a case in which the primary melanoma and five different metastases were studied by using cytogenetics.The primary tumor showed an inversion of chromosome 1 where the p36 region, often proposed in literature as the location of a melanoma susceptibility gene, was involved.Three cutaneous and one lymphonodal metastases presented the same nine clonal chromosomal aberrations. In particular, one is a further rearrangement of the marker present in the primary tumor; another is a deletion of the 9p21pter region in which the p16 gene is located.Our results can provide a contribution to the hypothesis of the location of a candidate gene for melanoma in the 1p36 region and can also underscore the role of the 9p21 region in the progression of melanoma.
The Localization of Glutathione Peroxidase in the Photoreceptor Cells and the Retinal Pigment Epithelial Cells of Wistar and Royal College of Surgeons Dystrophic Rats
Authors: AKEO KIYOSHI , TSUKAMOTO HIDEO , OKISAKA SHIGEKUNI , HIRAMITSU TADAHISA , WATANABE KEIICHI 
Published: July 2006

Introduction: If degenerating photoreceptor outer segments not phagocytized by RPE cells in the retina of Royal College Surgeons (RCS) rats were to undergo peroxidation, the distribution of glutathione peroxidase (GSH‐PO) in the mitochondria or cytoplasm of the retina might be altered. We evaluated the immunocytochemical localization of GSH‐PO to identify subcellular organelles in sections of the retinas of RCS rats.Methods: Immunoblot analysis confirmed the presence of GSH‐PO molecules in the retinas of RCS and Wistar rats aged 3 weeks. Sections were reacted with the F(ab) fragment of anti‐rat αGSH‐PO and then examined by laser scanning microscopy (LSM) and transmission electron microscopy (TEM).Results: The size of the GSH‐PO molecule in the retina was about 21 KD in the mitochondria and 23 KD in the cytosol in both strains of rats. LSM revealed fluorescent granules in the photoreceptor inner segments of the Wistar rats, and immunohistochemical TEM revealed GSH‐PO in the mitochondria of their photoreceptor inner segments and retinal pigment epithelial (RPE) cells. In the RCS rats, the degenerating photoreceptor outer segments were clearly seen to be positive for anti‐GSH‐PO by conventional light microscopy (CLM). However, the photoreceptor inner segments of the RCS rats were negative for staining with anti‐GSH‐PO by LSM, and no GSH‐PO could be detected in the mitochondria of the photoreceptor inner segments or RPE cells by immuno‐TEM.Conclusion: Degeneration of the photoreceptor outer segments induced mitochondrial damage in the photoreceptor inner segments, and as a result GSH‐PO shifted from the photoreceptor inner segments to the degenerating outer segments.
Automating the legal Reasoning Process: A Computer that uses Regulations and Statutes to Draft Legal Documents
Authors: Sprowl James A. 
Published: July 2006

Several years ago, the American Bar Foundation initiated a modest investigation into the feasibility of designing a computer that could automate the assembly of form legal documents such as wills, trusts, complaints, and the like. The investigation has since matured into a major research effort to design an entirely new kind of computational processor. A prototype processor has now been constructed, and it is undergoing field tests to determine whether it truly fulfills the specialized needs of the legal drafter.This article briefly traces the history of the project, explaining the motivation behind it and describing the role of the participants. The article then introduces a new language—the principal result of the research effort—that may be used to draft both form documents and statutes, regulations, and other “instructions” defining how form documents are to be assembled. The new language, a subset of English, is fully comprehensible both to attorneys and to a properly designed computational processor. An attorney or paraprofessional may redraft form legal documents, statutes, and regulations in the new language and then feed them directly into the computer; no conventional programming is required. The computer asks questions couched in language taken from the forms and statutes, performs any necessary computations, and draws any necessary legal conclusions. The computer then returns client-customized legal documents ready for court filing. The article tells how the new processor may be set up to perform even the most complex drafting tasks—even complex tax-return preparation. Particular emphasis is placed on having the computer force the attorney or paraprofessional to proceed in a highly organized fashion with the development of a complex delivery system so that the computer, and not the attorney or paraprofessional, keeps track of the complex linkages between the elements of the system as it evolves.In its concluding section, the article explores the possible impact of this new technology upon the legal profession and the public, and the author expresses his view that centralized systems set up by legal specialists to support the work of numerous nonspecialists may expand the areas in which the generalist may do competent work and may enable the general practitioner to buck the current trend toward specialization.
Lawyers and Localities: The Interaction of Community Context and Professionalism
Authors: Landon Donald D. 
Published: July 2006

Most studies of the legal profession, done in metropolitan settings, have indicated that the profession is characterized by deep divisions associated with varying practice patterns, professional status, and orientation. But the sources of division within the bar include more than the specialization and stratification that are typical of the bar in metropolitan settings. Community context also appears to be a source of division. It determines not only the character of a lawyer's cases and clients but also the manner in which practice is conducted. This comparison of practice patterns in Missouri of rural lawyers with those in a middle-sized city (Springfield) revealed that while the small town context protects the bar from the centrifugal forces of stratification found in larger settings, it also has elements that challenge the professional independence of the practitioner. The small town or rural practitioner is very much a part of the local life, and thus local opinion and values have a salience for practice patterns not typical of larger settings. In addition, these rural lawyers are less likely to be engaged exclusively in law practice.Overall, the data suggest that the legal profession is divided not only by specialization and its attendant internal stratification but also by the size and character of the local context. In contrast to the view that the profession is a homogeneous professional subculture, we found that the bar may better be thought of as a rather loose-knit group with tenuous collegial ties whose professionalism is under constant challenge from the community in which law practice occurs.
Framework for Analysis of Legal Mobilization: A Decision-Making Model
Authors: Zemans Frances Kahn 
Published: July 2006

The American legal system, structured in an entrepreneurial mode, relies upon the individual actor to personally evaluate the burdens and benefits of invoking the law on his or her own behalf. Without discounting the contribution to our understanding of legal mobilization which has been made by the access-to-justice movement, the author argues that focusing on the poor and the distribution of legal services has limited our understanding of the legal system.The article presents an alternative analytic framework for examination of citizen use of the law. The model of legal mobilization presented focuses on demands rather than needs, on citizens rather than lawyers or judges, on decision making rather than access, and on invoking the law rather than compliance with it. Drawing on the literature and available empirical evidence, the author attempts to analytically clarify the complex process of legal mobilization by organizing relevant variables into a decision-making model that focuses on the individual actor and the factors weighed in deciding whether and how to proceed in mobilizing the law.
Referring Discovery Tasks to Special Masters: Is Rule 53 a Source of Authority and Restrictions?
Authors: Brazil Wayne D. 
Published: July 2006

The author concludes that federal judges who want to appoint special masters to perform duties related to civil discovery may not look to the Federal Rules of Civil Procedure for authority to do so. By examining the historical backdrop against which the original rules were written, as well as the minutes of the proceedings of the first Advisory Committee, Brazil demonstrates that neither Rule 53 nor any other rule was designed to grant federal trial courts power to assign pretrial discovery tasks to special masters. In fact, the evidence the author marshalls shows that the original Advisory Committee explicitly rejected the idea that the Federal Rules should authorize even a limited role for special masters in connection with discovery depositions.Finding no authority for such appointments in the Federal Rules, the author turns to the judiciary's “inherent power.” Drawing principles from the seminal Supreme Court opinion in this area, Brazil infers that in some circumstances the courts' inherent authority is a sufficient premise for delegating discovery tasks to special masters. Noting that the reported cases contain no clear guidelines about when or how federal judges should use this authority in making pretrial appointments, Brazil concludes by calling for a new federal rule covering this important subject.
The Legislative Role of Juries
Authors: Levine James P. 
Published: July 2006

Is it possible that jurors, in the process of evaluating the evidence against a defendant, act to some degree as legislators by assessing the soundness of policies they perceive the criminal law to be supporting? To test the hypothesis that jurors reflect public opinion in making such judgments, the author correlated changes in public opinion on war policy with fluctuations in the proportion of jury trial defendants found guilty of violating selective service laws.It was found that juries convicted those accused of draft evasion at a higher rate when a war was in progress than during peacetime and that during the Korean and Vietnam wars the conviction rate was directly correlated with public approval of American military actions. A breakdown of regional differences showed that the South was somewhat more supportive of the Vietnam War and more prone to convict in selective service cases than the rest of the nation was, but the results of this comparative analysis were inconclusive. Although the correspondence between public opinion and verdict tendencies was far from perfect (perhaps in part because of the skewed composition of juries), the study does support the notion that jurors draw on prevailing popular sentiments about laws and the public policies the laws further.
No Job Too Small: Justice Without Bargaining in the Lower Criminal Courts
Authors: Schulhofer Stephen J. 
Published: July 2006

Plea bargaining and other informal disposition devices dominate case processing in the lower criminal courts. Consistently, studies have found such courts characterized by assembly-line procedures, deemphasis or disregard of formal due process rights, and guilty plea rates approaching 100% of all convictions. Caseload pressures, tendencies toward cooperation among courtroom participants and, above all, process costs of litigation that greatly outweigh the stakes in minor cases are widely thought to render informality and high guilty plea rates inevitable.
The study reported here challenges these assumptions. The author finds that in Philadelphia roughly one-fifth of all misdemeanor dispositions and one-half of all determinations of guilt are the result of genuine adversarial trials. He shows that an adversarial trial system need not impose prohibitive resource costs, even when extended to misdemeanor cases involving little likelihood of imprisonment, and that process costs will not deter defendants from invoking formal procedures when the court culture is committed to providing trials. Finally, the author argues that even routine misdemeanor cases benefit from guarantees of fairness and accuracy afforded by trial but unattainable when cases are processed by plea bargaining.
Referral Practices Among Lawyers: A Theoretical and Empirical Analysis
Authors: Spurr Stephen J. 
Published: July 2006

This article analvzes referral practices among lawyers. The hypothesis is that the market for legal services is “efficient,” that is, market forces enable plaintiffs in personal injury cases to maximize their recoveries, given the quality of lawyers representing defendants. If this market is indeed efficient, one would expect that legal claims which would benefit most from high-quality legal services would be assigned to high-quality lawyers. The evidence shows that referrals enable claims to be matched with lawyers in just that way. That is, through the mechanism of referrals, claims of greater intrinsic value are assigned to lawyers of higher quality.
The paper also provides a model of the decision a generalist must make: to litigate a case himself or refer it to a trial specialist. The implications of the model support the hypothesis that this market is efficient in the sense defined above.
The Right to Privacy Is Political: Power, the Boundary Between Public and Private, and Sexual Harassment
Authors: Roth Louise Marie 
Published: July 2006

This paper concerns the relationship between power and the ability to defend the night of privacy. The discourse of public and private spheres has shifted historically, engendering arbitrary and changing legal and cultural definitions of the boundary between public and private. Historic specifications of this boundary have become untenable as increasing numbers of women entered the paid labor force. Recent formulations define the boundary of privacy as an area within each individual's life. However, greater social power increases the ability to protect personal privacy because it offers the ability to define and protect the “private” from scrutiny.
After outlining the history of the shifting public/private boundary, this argument is applied to sexual harassment. Explicitly sexual types of harassment are related to the public/private boundary in two ways. First, they challenge the boundary itself, representing the occurrence of “private” conduct in the “public” sphere of work and education. Second, sexual harassment reveals the importance of social power in defining and defending one's privacy. Sexual harassment represents the extreme on a continuum of communication patterns between status unequals, and an invasion of the sexual privacy of the target.
The Legal Cartography of Colonization, the Legal Polyphony of Settlement: English Intrusions on the American Mainland in the Seventeenth Century
Authors: Tomlins Christopher 
Published: July 2006

This essay investigates the first century of English colonization of the North American mainland, concentrating on the charters and letters patent that proponents of western planning secured over the course of the century. The elaborated legalities of chartering should be understood as a technology of planning and design. Charters allowed projectors both to justify their pursuit of particular territorial claims and to establish, with some precision, the conceptions of the appropriate, familiar, desired order of things and people that would be imposed onto uncharted social and physical circumstance.The structures of authoritative sociolegal order planned by projectors encountered others implicit in the migrations of actual settlers. Investigating settlers' disagreement with and departure from projectors' designs, the essay discards common explanations—that these were inevitable corrections brought about by the intrusion of local environmental realities on English projectors' fantasies, or the realization of an implicit evolutionary logic of political development, or of legal reception. It argues that disagreements were more often the result of a collision of distinct English legal cultures brought, by migration, into an unavoidable proximity.The essay counterposes the paradigm of “colonization” to both “common law reception” and “bottom-up localism” analyses of the formation of early American legal culture. It proposes that “colonization” also resolves the discontinuity between early (colonial) and later (U.S.) American history.
Lawyers' Roles in Voluntary Associations: Declining Social Capital?
Authors: Heinz John P. , Schnorr Paul S. , Laumann Edward O. , Nelson Robert L. 
Published: July 2006

The extent and nature of lawyers' participation in civic life probably has important effects on the character of the community's activity and its outcomes. Where and how lawyers participate in voluntary associations may influence the ability of those organizations to function within the larger structure of American institutions.
This paper compares findings from two surveys of Chicago lawyers, the first conducted in 1975 and the second in 1994-95. Contrary to some expectations, the available evidence does not suggest that community activities of lawyers decreased. Moreover, lawyers' energies in 1995 appear to have been devoted more often to socially concerned organizations, those with a reformist agenda, than had been the case in 1975. The types of organizations with the greatest increase in activity were religious and civic associations. A smaller percentage of the respondents held leadership positions in 1995 than in 1975, but, because of a doubling in the number of lawyers, the best estimate is that the bar's absolute level of contribution to community leadership did not change greatly.
In both 1975 and 1995, a hierarchy of social prestige appears to have influenced the pattern of lawyers' community activities. Lawyers who had higher incomes, were middle-aged, were Protestants, and who had attended elite law schools were more likely to be active or leaders in most kinds of organizations. In ethnic and fraternal organizations, however, the elites of the profession had relatively low rates of participation, while government lawyers, solo practitioners, and graduates of less prestigious law schools predominated. Status hierarchies within the broader community—as well as social differences in taste, preference, or “culture”—clearly penetrate the bar.
Suppression of Cell Proliferation and Signaling Transduction by Connective Tissue Growth Factor in Non–Small Cell Lung Cancer Cells
Authors: Chien Wenwen , Yin Dong , Gui Dorina , Mori Akio , Frank Jonathan Mordechai , Said Jonathan , Kusuanco Donato , Marchevsky Alberto , McKenna Robert , Koeffler H. Phillip 
Published: July 2006

Abstract
Connective tissue growth factor (CTGF) is a secreted protein that belongs to CCN family. The proteins in this family are implicated in various biological processes, such as angiogenesis, adhesion, migration, and apoptosis. In this study, we explored the roles of CTGF in lung tumorigenesis. The expression levels of CTGF in 58 lung cancer samples were reduced by &gt;2 fold in 57% of the samples compared with matched normal samples using real-time reverse transcription-PCR. These results were confirmed by immunohistochemical staining for CTGF in normal lung epithelia and lung cancer. Cellular proliferation was inhibited in non–small cell lung cancer (NSCLC) cell lines NCI-H460, NCI-H520, NCI-H1299, and SK-MES-1 by CTGF overexpression. Partially purified CTGF suppressed lung cancer cell growth. The growth inhibition caused by CTGF overexpression was associated with growth arrest at G0-G1 and prominent induction of p53 and ADP ribosylation factor. Most interestingly, overexpression of CTGF suppressed insulin-like growth factor-I–dependent Akt phosphorylation and epidermal growth factor–dependent extracellular signal-regulated kinase 1/2 phosphorylation. In summary, NSCLC cells expressed decreased levels of CTGF compared with normal lung cells; this lower expression has an effect on lung cancer cell proliferation and its cellular response to growth factors. Our data suggest that CTGF may behave as a secreted tumor suppressor protein in the normal lung, and its expression is suppressed in many NSCLCs. (Mol Cancer Res 2006;4(8):591–8)
Baseline Gene Expression Predicts Sensitivity to Gefitinib in Non–Small Cell Lung Cancer Cell Lines
Authors: Coldren Christopher D. , Helfrich Barbara A. , Witta Samir E. , Sugita Michio , Lapadat Razvan , Zeng Chan , Barón Anna , Franklin Wilbur A. , Hirsch Fred R. , Geraci Mark W. , Bunn Paul A. 
Published: July 2006

AbstractTyrosine kinase inhibitors (TKI) of the epidermal growth factor receptor (EGFR) produce objective responses in a minority of patients with advanced-stage non–small cell lung cancer (NSCLC), and about half of all treated patients progress within 6 weeks of instituting therapy. Because the target of these agents is known, it should be possible to develop biological predictors of response, but EGFR protein levels have not been proven useful as a predictor of TKI response in patients and the mechanism of primary resistance is unclear. We used microarray gene expression profiling to uncover a pattern of gene expression associated with sensitivity to EGFR-TKIs by comparing NSCLC cell lines that were either highly sensitive or highly resistant to gefitinib. This sensitivity-associated expression profile was used to predict gefitinib sensitivity in a panel of NSCLC cell lines with known gene expression profiles but unknown gefitinib sensitivity. Gefitinib sensitivity was then determined for members of this test panel, and the microarray-based sensitivity prediction was correct in eight of nine NSCLC cell lines. Gene and protein expression differences were confirmed with a combination of quantitative reverse transcription-PCR, flow cytometry, and immunohistochemistry. This gene expression pattern related to gefitinib sensitivity was independent from sensitivity associated with EGFR mutations. Several genes associated with sensitivity encode proteins involved in HER pathway signaling or pathways that interrelate to the HER signaling pathway. Some of these genes could be targets of pharmacologic interventions to overcome primary resistance. (Mol Cancer Res 2006;4(8):521–8)