bluuebunny/crossref_metadata_2025_split huggingface dataset:
Community structure of soil microorganisms and endophytes of honeysuckle at different ecological niche specificities
Authors: Xu Qingyi , Jiang Dan , Zhou Na , Kang Yingquan , Li Meng , Yang Chuchu , Liu Xinyu , Mi Jiu , Hua Guodong , Ren Guangxi , Liu Chunsheng 
Published: March 2024

Abstract
Background
The plant microbiome is one of the key determinants of healthy plant growth, and it has many functions, such as nitrogen fixation, iron production, phosphorus dissolution, and auxin production. However, the structural composition of the bacterial and fungal microbiomes in the plant microenvironment in different regions, especially the relationship between subsurface and aboveground communities, is complex and not fully understood. The present study investigated the niche differentiation of plant microbiomes in different regions and the diversity of microbial community structure within different niches.
Methods
High-throughput sequencing was used to sequence the soil and endophytes of honeysuckle. Alpha diversity analysis and principal component analysis (PCoA) were used to study the community composition structure of the soil and endophyte, and the function of endophyte bacteria and fungi was predicted based on the PICRUST2 process and FUNGuild.
Results
In total, there were 382 common bacterial genera and 139 common fungal genera in the soil of different producing areas of honeysuckle. There were 398 common bacterial genera and 157 common fungal genera in rhizosphere soil. More beneficial bacteria were enriched in rhizosphere soil. Endophytic bacteria were classified into 34 phyla and 770 genera. Endophytic fungi were classified into 11 phyla and 581 genera, among which there were significant differences in the dominant genera of roots, stems, leaves, and flowers, as well as in community diversity and richness. PCoA indicated that the community structure of endophytic bacteria and fungi among roots, stems, leaves, and flowers was also significantly different. Bacterial community function prediction demonstrated that the relative abundance of functional genes involved in the metabolism of endophytic bacteria was the highest, and the functions of endophytic fungi were mainly related to saprophytic bacteria. In different tissues of honeysuckle, functional genes that could fight microorganisms were also found in KEGG secondary functional genes.
Conclusion
There are many common bacterial genera and fungi genera in the soil and rhizosphere soil of honeysuckle. The endogenous dominant core bacteria in each part of honeysuckle plant have a high degree of overlap with the dominant bacteria in soil. Functional prediction suggested that some dominant core bacteria have antibacterial effects, providing scientific basis for further exploring the microorganisms with antibacterial function of honeysuckle to understand the medicinal value of honeysuckle.
Audio fault diagnosis of belt conveyors based on improved variational modal decomposition and improved adaptive noise reduction convolutional network in strong noise environment
Authors: Li Zhiyuan , Wang Hongwei , Liang Wei , Yao Linhu 
Published: March 2024

Abstract

Fault diagnosis of belt conveyors is crucial for coal mine production, but audio-based fault diagnosis in underground coal mines remains challenging due to the strong noise environment. To address this problem, a method for audio fault diagnosis of belt conveyors based on improved variational modal decomposition and improved adaptive noise reduction convolutional networks in a strong noise environment is proposed. Firstly, the improved beluga whale optimization is designed by introducing the non-linear balance factor and non-linear probability and combining them with the proposed cyclical shock factor to optimize the variational modal decomposition parameters to achieve noise reduction and signal reconstruction. Secondly, an improved adaptive noise reduction convolutional network is developed using an adaptive threshold activation function and an improved loss function to enhance noise robustness and fault diagnosis accuracy. Finally, the proposed method's effectiveness is evaluated in low and strong noise environments, with experimental results demonstrating superior fault diagnosis performance. In low noise environments, the fault diagnosis accuracy is 98.61%, and in strong noise environments, it is 98.96%, outperforming existing fault diagnosis methods.
An Investigation of the Prevalence of Stress, Anxiety, and Depression and their Relationship with Some Demographic Variables in Secondary High School Female Students
Authors: GHsabe Nasibe Farmani , Garmaroudi Gholamreza , Leyli Ehsan Kazemnezhad , Farrahi Hassan 
Published: March 2024

Abstract

Background
Given that mental health is one indicator of health evaluation in different societies and that high school students, especially girls, face many stressors, this study aims to investigate the prevalence of stress, anxiety, and depression and their relationship with some demographics variables which conducted in secondary high school female students.
Materials and methods
In this descriptive-analytical study, 1,181 secondary school girls of Rasht city were included in the study by cluster sampling method. The research was done using the DASS-21 questionnaire and demographic variables questionnaire. Data analysis was done with SPSS-21 software and statistical tests.
Results
A large number of students were suffering from moderate to severe stress, anxiety, and depression. The investigated variables were the history of mental illnesses in the family, the number of students in the class, the education and occupation of the parents, the economic status, the type of school, green space, the attractiveness of colors and materials, and sufficient lighting in the classroom which are related to these three types of disorders. In the results of logistic regression, the predictor variables of stress, anxiety, and depression were having green spaces in the school, sufficient lighting, and the uniqueness of the colors and materials used in the students' classrooms.
Conclusion
Conclusion: Considering the high prevalence of stress, anxiety, and depression among students and the correlation of some changeable demographic variables with these mental disorders, efforts to create political, social, and cultural contexts and carry out educational interventions to improve the mental health of students are a serious necessity.
The impact of Sports Social Support, Sports Behavior, and Impulsive Personality on Academic Adaptation in Adolescents
Authors: Liu Li , Zhang Tingran , Liu Yongsen , Luo Jiong 
Published: March 2024

Abstract
Background
Currently, Chinese teenagers are facing serious academic adaptation problems, which have affected their physical and mental health development.
Objective
To explore the effects of exercise behavior, social support for exercise, and impulsive personality on academic adaptation in adolescents, and to provide theoretical and practical references for adolescents to overcome academic adaptation barriers and improve academic performance.
Method
Adolescents in Chongqing, China were selected as the survey subjects, and relevant data was obtained through a self-report questionnaire. SPSS 21.0 and AMOS 19.0 statistical analysis software were used to process and analyze the data.
Result
Engaging in high contact - high-attack sports, participating in school sports teams, duration of each exercise, and impulsive personality can all induce academic adaptation problems in adolescents, while participating in sports cannot effectively reduce the occurrence of academic adaptation problems; Parental support has a significant positive effect on adolescent academic adaptation problems, but impulsive personality is a key factor in inducing academic adaptation problems.
Conclusion
Impulsive personality is a key factor affecting academic adaptation problems. Adolescents may seek stimulation through high exposure and aggressive exercise, while forgetting the frustration of academic maladaptation, making exercise participation a means of reducing academic adaptation obstacles ineffective.
Observational Study of Left Bundle Branch Area Pacing: Implantation of the Solia S Lead using the Selectra 3D Sheath at an Inclined Angle
Authors: Ju Changlin , Zhou YU , Cheng Keng , Xie Xiangrong 
Published: March 2024

Abstract

Background
Left Bundle Branch Area Pacing (LBBaP) is a cardiac pacing technique designed to mimic the natural conduction system of the heart. Traditional right ventricular apical pacing has been associated with increased risks of heart failure and atrial fibrillation. This study investigates the stability and safety of LBBaP using the Selectra 3D sheath (Biotronik) with an inclined angle for implanting the Solia S lead (Biotronik, SE & Co, KG).
Methods
A single-center retrospective study was conducted on 25 patients who underwent LBBaP implantation using the Selectra 3D sheath at our hospital. The procedure involved inserting the Solia S lead into the interventricular septum at an inclined angle. Surgical and postoperative data were collected, including the success rate, depth and angle of electrode insertion, complications, and follow-up data.
Results
The success rate of LBBaP implantation was 92%. The length of electrode insertion into the interventricular septum ranged from 12 to 23.0mm, with an average of 18.1 ± 3.08mm. The angle formed between the electrode and the septum ranged from 0° to 57.3°, with an average of 35.14°±14.31°. During the 3-month follow-up period, pacing parameters remained stable, and no complications were reported.
Conclusions
LBBaP implantation using the Selectra 3D sheath with an inclined angle for the Solia S implantation demonstrates stability and safety. The procedure boasts a high success rate and offers an effective option for LBBaP implantation.
Brain metabolic patterns and amyloid brain deposition at 5 years follow-up in patients with amnestic mild cognitive impairment as assessed by 11 C-PIB and 18 F-FDG PET/CT
Authors: Jiménez-Bonilla Julio Francisco , De Arcocha-Torres Maria , Lage Carmen , López-García Sara , Martínez-Rodríguez Isabel , Rodríguez-Rodríguez Eloy , Sánchez-Juan Pascual , Quirce Remedios 
Published: March 2024

Abstract

Background: To assess 5-year changes in regional brain metabolic patterns by 18F-FDG PET/CT in amnestic mild cognitive impairment (A-MCI) patients and link these changes to clinical outcomes and initial 11C-PIB PET/CT findings.
Methods: In 65 A-MCI patients diagnosed using 11C-PIB and 18F-FDG PET/CT, a 5-year follow-up began. 34 patients underwent new 11C-PIB and 18F-FDG PET/CT scans, analyzing global and regional brain amyloid load (BAL) and glucose brain metabolism (GBM) across 26 selected brain regions. Clinical status was assessed using the Global Deterioration Scale (GDS) and neuropsychological tests. Additionally, 73 healthy volunteers formed the control group, undergoing 18F-FDG PET/CT scans.
Results: Follow-up at 5 years involved 65 A-MCI patients. Among the 34 re-evaluated (27 PIB positive, 7 negative initially), global BAL increased by 22% and GBM decreased by 1.4%, notably in specific brain regions. In the 27 PIB positive patients, two cerebral metabolic patterns emerged: 13/27 showed reduced global GBM (mean: -11.25%) and 14/27 showed no reduction (mean: 8.80%) (p<0.05), with regional variations. Moderate correlations were found between regional BAL and GBM (r= -0.378 and 0.336). Clinical progress at 5 years for PIB positive A-MCI (GDS3): one remained A-MCI, 10 developed mild-AD (GDS4), 10 moderate-AD (GDS5), and 6 severe-AD (GDS6). Among the 7 PIB negative A-MCI (GDS3), 5 remained GDS3, and 2 developed other dementias.
Conclusions: At 5 years, A-MCI patients with brain amyloid showed diverse glucose metabolic changes, involving global and regional hypermetabolism linked to varying cognitive statuses.
Novel loss of function variant in SOST from Chinese family results in van Buchem disease
Authors: Guo Yufan , Wu Xintao , Gu Yu , Feng Jianhua 
Published: March 2024

Abstract

Background
SOST encodes a secreted glycoprotein that is similar in sequence to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function variants of SOST result in sclerosteosis, Van Buchem disease (VBD), or craniodiaphyseal dysplasia. SOST-related genetic disorders are highly rare, and only limited studies have reported variants associated with VBCH.
Methods
Clinical data such as magnetic resonance imaging (MRI), computed tomography (CT), emission computer tomography (ECT), electromyogram (EMG), routine blood tests, and physical examinations were collected from the proband. Trio-whole exome sequencing (Trio-WES) of this family was performed on the family, and all variants with a minor allele frequency (< 0.01) in the exon and canonical splicing sites were selected for further pathogenic evaluation. Candidate pathogenic variants were validated through Sanger sequencing. The wild-type and variant of SOST were cloned into an expression vector, and the RNA and protein expression levels were investigated in the HEK293T cell line.
Results
In this study, we present a case study of a proband who displays abnormal facial expressions accompanied by numbness. The results of the brain MRI show thicening of the skull and disapearance of the diplopia signal. The temporal bone CT scan indicates diffuse osteosclerosis affecting the bilateral ossicular chains and internal auditory meatus, as well as stenosis of the bilateral internal auditory meatus. Trio-WES sequencing detected a new homozygous variant in the proband on SOST gene [NM_025237.3: c.327C > A, (p.Cys109*)], which was also validated in his sister from the same family. According to the ACMG guidelines, this variant is classified as likely pathogenic (LP). Further in vitro experiments demonstrated that the novel detected variant caused a decrease in SOST expression at the RNA and protein level by producing a truncated protein.
Conclusion
The report presents new evidence for the clinical diagnosis of SOST-related facial numbness and expands the variant spectrum of SOST.
The clinical value and cost effectiveness of a 6-month digital health intervention to improve physical activity and mental health-related quality of life in people with chronic kidney disease (Kidney BEAM): A multi-centre, randomised controlled trial
Authors: Greenwood Sharlene , Briggs Juliet , Young Hannah , Castle Ellen , Billany Roseanne , Mangahis Emmanuel , Walklin Christy , Haggis Lynda , Asghari Elham , Bhandari Sunil , Bishop Nicolette , Bramham Kate , Burton James , Campbell Jackie , Chilcot Joseph , Cooper Nicola , Deelchand Vashist , Graham-Brown Matthew , Hamilton Alexander , Jesky Mark , Kalra Philip , Koufaki Pelagia , Mccafferty Kieran , Nixon Andrew , Noble Helen , Saynor Zoe , Taal Martin , Tollitt James , Wheeler David , Wilkinson Thomas , Worboys Hannah , Macdonald Jamie 
Published: March 2024

Abstract
There is inequity in provision of physical rehabilitation services for people living with chronic kidney disease (CKD). The Kidney BEAM trial evaluated the clinical value and cost effectiveness of a physical activity digital health intervention in CKD. In a single-blind, 11 centre, randomised controlled trial, 340 adult participants with CKD were randomly assigned to either the Kidney BEAM physical activity digital health intervention or a waitlist control. The primary outcomes were the difference in the Kidney Disease Quality of Life Short Form 1.3 Mental Component Summary (KDQoL-SF1.3 MCS) between intervention and control groups at 6 months, and cost-effectiveness. At 6 months there was a significant difference in mean adjusted change in KDQoL MCS score between Kidney BEAM and waitlist control (intention-to-treat adjusted mean: 5.9 {95% confidence interval: 4.4 to 7.5} arbitrary units, p<0.0001), and a 93% and 98% chance of the intervention being cost-effective at a willingness to pay threshold of £20,000 and £30,000 per quality-adjusted life year gained. In conclusion, the Kidney BEAM physical activity digital health intervention is a clinically valuable and cost-effective means to improve mental health related quality of life in people with CKD (trial registration no. NCT04872933).
Triplet-Induced Stable Organic Radical Emitter in Host-Guest Room-Temperature Phosphorescent Materials
Authors: Lei Yunxiang , Li Gengchen , Zhang Yongfeng , Zhao Yeyun , Cheng Xin , Wang Ruiyang , Dai Wenbo , Wang Li , Sun Peng , Shi Jianbing , Tong Bin , Cai Zhengxu , Dong Yuping , Wang Yijia , Zheng Yuan 
Published: March 2024

Abstract

New strategies for the synthesis of stable organic luminescent radicals with their unique electronic structures are highly desirable. Herein, we designed and synthesized a novel arylboron compound BN-2Ph with radical-generating potential. BN-Ph was used as the guest molecule in the host-guest system, exhibiting high-efficiency and persistent phosphorescent emission. The long lifetime triplet exciton induced unexpected luminescent radicals with red emission in the doped crystalline state, which could be rapidly and reversibly generated by heating and recrystallizing. The quantitative electron paramagnetic resonance analysis and theoretical calculations suggest that the triplet-stabled photoinduced electron transfer mechanism between host and guest molecules is responsible for the generation and stabilization of radicals. This doped system was applied in AND and OR logic gates, showing its potential application in logic circuits. Our results provide a simple molecular-design strategy for achieving stable organic luminescent radicals.
Prioritization and Identification of Accident Black Spots and Analyzing Road Traffic Accidents in the Amhara Region: A Case Study from Dembecha to the Injibara Highway Segment
Authors: Geremew Gedefaye , Geremew Gedefaye 
Published: March 2024

Abstract
Millions of people die in road accidents annually, causing significant damage to the economy and humanity. Even if severe road traffic accidents are a major health concern worldwide, unlike in developed or high-income countries, very little progress has been made in addressing this problem in many developing countries. Ethiopia is one of the developing countries with the worst road traffic accident records in the world, and it ranks second among East African countries. Nonetheless, there are ways to strengthen the reduction of injured persons that will help to lower the toll from severe RTAs. The main focus of the study is to analyze road accidents through black spot prioritization and identification as well as to analyze road traffic accidents on the Dembecha-Injibara (99 km) highway segment. The selected sites for the present study included around Dembecha, Dembecha, Around Finote Selam, Finote Selam city, Burie, Guagusa Shikudad, Banja and Injibara city. This research was established mainly through descriptive and inferential analysis. Inferential analysis was carried out using the quality control method (Zegeer: 1982) for accident rate calculations, critical accident rates were calculated by using the Poisson distribution analysis method, and the results were generated. Furthermore, the analysis of the prioritization of accident black spots was carried out by considering the weight of factors that lead to road traffic accidents and the weight of road traffic accident severity types. Accordingly, black spot location and priority value analyses were performed. Therefore, out of the 8 sections of the subject road, Finote Selam, Banja and Burie were highly prone to accidents, and the corresponding accident frequency values were 94, 78 and 69, respectively, while the total accident factor weightage values were 34, 37 and 31, respectively. Moreover, the priority values of Burie, around Finote Selam and Banja are 27.22, 21.57 and 20.57, respectively. As a result, Burie Road was found to be the prime accident-prone location, followed by the location around the Finote Selam and Banja road segments. Based on the findings, recommendations and suggestions were provided on how to reduce the severity of traffic accidents in the study area.
Estimates of heterosis, inbreeding depression and transgressive segregation in rice (Oryza sativa L.) under sodic soil
Authors: Shrivastav Shiv Prakash , Verma O. P. , Jakhar Dan Singh 
Published: March 2024

Abstract
The present investigation was carried out at the Main Experimental Station of Acharya Narendra Deva University of Agriculture & Technology, Narendra Nagar (Kumarganj), Ayodhya (U.P.) India. A field experiment was conducted by using a line x tester set of 63 F1s and 63 F2s derived by crossing 21 rice genotypes/varieties as lines (females) with three testers (males) viz., Narendra Usar Dhan 3, CSR 23 and IR 28 with 2 check varieties (Jaya and CSR 43) of rice (Oryza sativa L.) in randomized complete block design with three replications to work out the heterosis, transgressive segregantion and inbreeding depression effects for various attributes under the sodic soil condition. Among these, top 5 F1s viz., NDRK 5037 x Narendra Usar Dhan 3, NDRK 5062 x IR 28, NDRK 5062 x CSR 23, NDRK 5037 x CSR 23 and NDRK 5040 x Narendra Usar Dhan 3were showed significant positive standard heterosis for grain yield per plant over SV1 and SV2, respectively. All of the above mentioned crosses had highly significant inbreeding depression for grain yield per plant in F2 generation. Inspite of grain yield of these F1s had significant heterosis and inbreeding depression for some of the other yield contributing characters also. This study indicated the presence of non additive gene action in the inheritance of grain yield per plant and some of the other yield contributing characters. Tolerant breeding populations showed similar banding pattern whereas susceptible exhibited similar banding pattern but possesses wide variations between tolerant and susceptible. At 35 kDa the medium to dark bands were present in parents, F1s, F2s, transgressive segregants and checks while in highly inbreeding depressed cross combinations, variable range of the bands were observed viz., absence of bands, light, medium and dark bands. Our data ofer a valuable resource for advancing the understanding and facilitating the utilization of additive and non-additive information for rice improvement.
Concurrent afatinib and stereotactic body radiotherapy improved survival of patient with oligometastatic EGFR-mutated non-small cell lung cancer： A case study and literature review
Authors: Sun Lisi , Tao Dan , Lv Yuyu , Wu Yongzhong , Wang Chunyu , Yue Xie 
Published: March 2024

Abstract
Background: Epidermal growth factor receptor (EGFR) mutated patients treated with target therapy are inevitable to develop resistance to tyrosine kinase inhibitors (TKIs). It has recently been proved that concurrent stereotactic body radiotherapy (SBRT) and the first-generation TKIs can prolong both progression-free survival (PFS) and overall survival (OS) of EGFR-mutated patients with limited metastases. However, the efficacy and safety of concomitant second-generation TKIs and SBRT is still unknown.
Case presentation: We for the first time present a stage IVA patient with mutation of both EGFR G719X and L861Q, who after initial response, had developed intracranial progression during afatinib monotherapy. With local treatment for the brain metastasis, she continued to receive afatinib and then a concurrent consolidative lung SBRT. Until January 2023, the patient had achieved a PFS of 24 months and OS of 32 months without serious adverse events except for a grade 1 radiation pneumonitis after the lung SBRT.
Conclusions: With this case and a literature review, we aim to demonstrate that concurrent afatinib and SBRT can bring prognostic benefits to oligometastatic NSCLC patients with uncommon EGFR mutations with good tolerance.