bluuebunny/crossref_metadata_2025_split huggingface dataset:
Component Analysis of HPLC Profiles of Unique Lipoprotein Subclass Cholesterols for Detection of Coronary Artery Disease
Authors: Okazaki Mitsuyo , Usui Shinichi , Fukui Akio , Kubota Isao , Tomoike Hitonobu 
Published: September 2006

AbstractBackground: Patients with coronary artery disease (CAD) are known to have several lipoprotein abnormalities. We examined plasma cholesterol concentrations of major lipoproteins and their subclasses, using a gel permeation HPLC, to establish an association between a lipoprotein subclass pattern and the presence of CAD.Methods: We performed a simple and fully automated HPLC, followed by mathematical treatment on chromatograms, for measuring cholesterol concentrations of major lipoproteins and their subclasses in 62 male patients (45 with CAD and 17 controls without CAD) who underwent cardiac catheterization.Results: For major lipoprotein classes, the patient group had a significantly (P &lt;0.05) higher LDL-cholesterol (LDL-C) and lower HDL-cholesterol (HDL-C), but no difference in VLDL-cholesterol (VLDL-C) concentrations. For lipoprotein subclasses, the patient group had a significantly higher small VLDL-C (mean particle diameter of 31.3 nm, P &lt;0.001), small LDL-C (23.0 nm, P &lt;0.05), and very small LDL-C (16.7–20.7 nm, P &lt;0.001), but a significantly lower large HDL-C (12.1 nm, P &lt;0.001) concentrations. Combined variables of “small VLDL-C + small LDL-C + very small LDL-C – large HDL-C” differentiated the patient from the control group more clearly than single-subclass measurements or calculated traditional lipid markers.Conclusions: These results suggest the usefulness of multiple and simultaneous subclass analysis of proatherogenic and antiatherogenic lipoproteins and indicate that HPLC and its component analysis can be used for easy detection and evaluation of abnormal distribution of lipoprotein subclasses associated with CAD.
Factors Affecting S-Homocysteinylation of LDL Apoprotein B
Authors: Zinellu Angelo , Zinellu Elisabetta , Sotgia Salvatore , Formato Marilena , Cherchi Gian Mario , Deiana Luca , Carru Ciriaco 
Published: September 2006

AbstractBackground: Hyperhomocysteinemia is an important risk factor for vascular disease and atherosclerosis, but the mechanisms by which homocysteine exerts its deleterious effects are not known. Because oxidation and/or homocysteinylation may increase atherogenicity of LDL, we investigated S-homocysteinylation of LDL as a possible contributor to atherosclerosis pathogenesis.Methods: We used capillary electrophoresis to measure LDL-bound thiols [homocysteine, cysteine (Cys), cysteinylglycine, glutathione, and glutamylcysteine] in 104 healthy study participants We also assessed total plasma thiol concentrations and lipid profiles.Results: Our data suggest that apoprotein B (apoB)-cysteinylglycine (CysGly), apoB-Hcy, and apoB-Cys concentrations are markedly higher in men than in women. The percentage of CysGly and glutathione on apoB was higher than that of the same thiols in plasma, whereas the other thiols were markedly less prevalent in lipoprotein than in plasma. Pearson correlation showed that among all thiols, only total plasma Hcy is related to apoB-Hcy concentrations. Multiple correlation analysis confirmed that total Hcy was the most important determinant of apoB-Hcy. Age and LDL cholesterol also showed positive associations, but Cys and, mainly, CysGly were negatively associated with apoB-Hcy concentrations.Conclusions: apoB-Hcy derivative formation is mainly dependent on total homocysteine concentration. Increased cholesterol concentrations are related to increased apoB-Hcy. CysGly seems to compete with Hcy for binding to LDL apoprotein, suggesting that CysGly may protect against atherosclerosis by decreasing the concentrations of Hcy transferred by LDL from plasma to endothelial and subendothelial spaces.
High-Speed Interferometric Detection of Label-Free Immunoassays on the Biological Compact Disc
Authors: Zhao Ming , Nolte David , Cho Wonryeon , Regnier Fred , Varma Manoj , Lawrence Greg , Pasqua John 
Published: September 2006

Abstract
Background: We describe a direct-detection immunoassay that uses high-speed optical interferometry on a biological compact disc (BioCD).
Methods: We fabricated phase-contrast BioCDs from 100-mm diameter 1.1-mm thick borosilicate glass disks coated with a 10-layer dielectric stack of Ta2O5/SiO2 that serves as a mirror with a center wavelength at 635 nm. The final layer is a λ/4 layer of SiO2 onto which protein patterns are immobilized through several different chemical approaches. Protein on the disc is scanned by a focused laser spot as the disc spins. Interaction of the light with the protein provides both a phase-modulated signal and a local reference that are combined interferometrically to convert phase into intensity. A periodic pattern of protein on the spinning disc produces an intensity modulation as a function of time that is proportional to the surface-bound mass. The binding of antigen or antibodies is detected directly, without labels, by a change in the interferometric intensity. The technique is demonstrated with a reverse assay of immobilized rabbit and mouse IgG antigen incubated against anti-IgG antibody in a casein buffer.
Results: The signal increased with increased concentration of analyte. The current embodiment detected a concentration of 100 ng/L when averaged over ∼3000 100-micron-diameter protein spots.
Conclusions: High-speed interferometric detection of label-free protein assays on a rapidly spinning BioCD is a high-sensitivity approach that is amenable to scaling up to many analytes.
Chip Electrophoresis as a Method for Quantifying Total Microalbuminuria
Authors: Chan Owen TM , Herold David A 
Published: September 2006

AbstractBackground: Microalbuminuria is an important prognostic marker in diabetic nephropathy and cardiovascular disease. Initially, most commercial assays used immunoreactivity to quantify microalbuminuria; however, size-exclusion HPLC demonstrated the existence of nonimmunoreactive forms of albumin that may not be detected by immunoassay. Recent liquid chromatography tandem mass spectrometry analyses suggested that size-exclusion HPLC gave higher results attributable to other urine proteins coeluting with albumin. We describe an assay that measures total microalbuminuria (immunoreactive and nonimmunoreactive) without any discernable interference from other common urine proteins.Methods: We used an automated chip electrophoresis system that utilized microfluidic separation technology and fluorescent sample detection. Each albumin specimen was mixed with the manufacturer’s sample buffer in addition to a chicken albumin internal calibrator and then electrophoresed without additional reducing agents.Results: With variable concentrations of bovine serum albumin normalized to a chicken albumin internal calibrator, the electrophoresis system was best fit with a polynomial (R2 = 0.9997; concentration range, 5–300 mg/L). The lower limit of detection was 5 mg/L. Interchip and intrachip variation studies conducted on patient urine demonstrated CVs of 3%–13%. The introduction of potentially interfering agents (i.e., molecular analytes, nonalbumin proteins) did not alter precision. Compared with immunoassay, the chip electrophoresis identified higher microalbuminuria concentrations in all urine samples. The method also clearly resolved the albumin peak from interfering proteins.Conclusions: Unlike immunoassay, chip electrophoresis can detect both immunoreactive and nonimmunoreactive forms of albumin. This system is a simple, robust method to quantify microalbuminuria with good sensitivity, precision, and accuracy.
Association of Adiponectin Gene Variations with Risk of Incident Myocardial Infarction and Ischemic Stroke: A Nested Case-Control Study
Authors: Hegener Hillary H , Lee I-Min , Cook Nancy R , Ridker Paul M , Zee Robert YL 
Published: September 2006

Abstract
Background: Adiponectin (ADIPOQ) gene variations are associated with risk of cardiovascular disease in patients with diabetes. No prospective data are available, however, on the risk of atherothrombotic disorders in persons with ADIPOQ variations who do not have diabetes.
Methods: From a group of DNA samples collected at baseline in a prospective cohort of 14 916 initially healthy American men, we assessed the presence of 5 ADIPOQ genetic variants (rs266729, rs182052, rs822396, rs2241766, and rs1501299) in samples from 600 Caucasian men who subsequently suffered an atherothrombotic event (incident myocardial infarction or ischemic stroke) and from 600 age- and smoking-matched Caucasian men who remained free of reported vascular disease during follow-up (controls).
Results: Genotype distributions for the variations tested were in Hardy-Weinberg equilibrium. Marker-by-marker conditional logistic regression analysis, adjusted for potential risk factors, showed an association of rs266729 [recessive: odds ratio (OR), 0.26; 95% confidence interval (CI), 0.10–0.64; P = 0.004] and rs182052 (recessive: OR, 0.40; 95% CI, 0.21–0.76; P = 0.006) with decreased risk of ischemic stroke. These findings remained significant after Bonferroni correction. Haplotype-based (constituted by rs266729, rs182052, and rs822396) conditional logistic regression analysis, adjusted for the same potential risk factors, showed an association of haplotype G-A-G (OR, 0.28; 95% CI, 0.09–0.87; P = 0.03) with decreased risk of ischemic stroke. Prespecified analysis limited to participants without baseline diabetes showed similar significant findings.
Conclusions: The present prospective investigation provides further evidence for a protective role of adiponectin gene variation in the risk of ischemic stroke that was independent of the presence of diabetes.
Measuring Radial Total Stresses on Model Suction Caissons in Clay
Authors: Chen W , Randolph MF 
Published: September 2006

Abstract
Miniature total pressure transducers (TPTs) were incorporated on model suction caissons in order to measure changes in radial stress during installation and loading of the caissons. The accuracy of TPTs in clay was first assessed by calibration tests at both 1g and in a geotechnical centrifuge at up to 120g. Calibration tests in a modified triaxial apparatus showed that the accuracy of the TPTs under loading, unloading, cyclic loading, and sustained loading was better than 92.5%. The initial readings of the TPTs were also found to change only slightly on transfer among air, water, and soil, in spite of differences in the heat exchange environment. The cross-sensitivity of TPTs to an axial load of 300 N on the caisson was insignificant. The accuracy of TPTs in water in the centrifuge was better than 96%. The TPTs also gave reasonable measurements of radial stresses generated on the outer wall of suction caissons during penetration in clay at 120g, as assessed by comparison with stress changes predicted using the strain path method.
Molecular Profiling of CD34+ Cells in Idiopathic Myelofibrosis Identifies a Set of Disease-Associated Genes and Reveals the Clinical Significance of Wilms' Tumor Gene 1 (WT1)
Authors: Guglielmelli Paola , Zini Roberta , Bogani Costanza , Salati Simona , Pancrazzi Alessandro , Bianchi Elisa , Mannelli Francesco , Ferrari Sergio , Le Bousse-Kerdilès Marie-Caroline , Bosi Alberto , Barosi Giovanni , Migliaccio Anna Rita , Manfredini Rossella , Vannucchi Alessandro M. 
Published: September 2006

Abstract
This study was aimed at the characterization of a gene expression signature of the pluripotent hematopoietic CD34+ stem cell in idiopathic myelofibrosis (IM), which would eventually provide novel pathogenetic insights and/or diagnostic/prognostic information. Aberrantly regulated genes were revealed by transcriptome comparative microarray analysis of normal and IM CD34+ cells; selected genes were also assayed in granulocytes. One-hundred seventy four differentially expressed genes were identified and in part validated by quantitative polymerase chain reaction. Altered gene expression was corroborated by the detection of abnormally high CD9 or CD164, and low CXCR4, membrane protein expression in IM CD34+ cells. According to class prediction analysis, a set of eight genes (CD9, GAS2, DLK1, CDH1, WT1, NFE2, HMGA2, and CXCR4) properly recognized IM from normal CD34+ cells. These genes were aberrantly regulated also in IM granulocytes that could be reliably differentiated from control polycythemia vera and essential thrombocythemia granulocytes in 100% and 81% of cases, respectively. Abnormal expression of HMGA2 and CXCR4 in IM granulocytes was dependent on the presence and the mutational status of JAK2V617F mutation. The expression levels of both CD9 and DLK1 were associated with the platelet count, whereas higher WT1 expression levels identified IM patients with more active disease, as revealed by elevated CD34+ cell count and higher severity score. In conclusion, molecular profiling of IM CD34+ cells uncovered a limited number of genes with altered expression that, beyond their putative role in disease pathogenesis, are associated with patients' clinical characteristics and may have potential prognostic application.
Co‐witness feedback in line‐ups
Authors: Skagerberg Elin M. 
Published: September 2006

AbstractThe aim of the current study was to establish whether feedback from a co‐witness concerning their choice of suspect could influence an individual witness' certainty and other testimony‐relevant judgements. Eighty‐two university students and members of the general public viewed a film of a staged mugging in pairs and then made an identification of who they thought was the suspect from a culprit‐absent line‐up (i.e. identification parade). The participants were then required to tell their partner whom they had identified and to fill out a questionnaire with testimony‐relevant questions (e.g. How good a view did you get of the person in the line‐up?). When the pairs of participants agreed on their choice of suspect, their scores on the testimony‐relevant questions tended to be higher than when the pairs did not agree. This shows that co‐witnesses can influence each others' memory reports when giving each other feedback after the identification process. The implications of these findings are discussed. Copyright © 2006 John Wiley & Sons, Ltd.
Effects of magnetic field on the antioxidant enzyme activities of suspension‐cultured tobacco cells
Authors: Sahebjamei Hassan , Abdolmaleki Parviz , Ghanati Faezeh 
Published: September 2006

AbstractEffects of magnetic fields (MFs) on the activities of antioxidant enzymes of suspension‐cultured tobacco cells were investigated. Compared with the control cells, exposure of the cells to static MF with the magnitudes of 10 and 30 mT for 5 days, 5 h each day, increased the activity of superoxide dismutase (SOD). In contrast, the activity of the catalase (CAT) and ascorbate peroxidase (APX) was decreased by MF, compared with those of the control cells. Level of lipid peroxidation was also increased by MF. It suggests that MF could deteriorate antioxidant defense system of plant cells. Bioelectromagnetics. © 2006 Wiley‐Liss, Inc.
Survey of residential 50 Hz EMF exposure from transformer stations
Authors: Szabó Judit , Jánossy Gábor , Thuróczy György 
Published: September 2006

AbstractIn Hungary it is typical that 10/04 kV transformer stations are being installed in multistory residential and office buildings. Magnetic fields (MFs) up to several tens of µT have been measured in apartments close to transformers. The aim of the present study was to provide systematic assessment of MF exposure of residents living above transformer stations. Out of 41 addresses provided by the electricity supplier, current load of 21 transformers and MF in 21 apartments was measured. Spot MFs at 1 m height and time weighted average 24 h MF exposure at bed height was measured. All‐day personal MF exposure was measured at waist and HOME exposure was calculated. BED exposure was measured at bed height. Participants kept a time‐activity diary. The time‐weighted average 24 h MF exposure (3.03 µT) exceeded the usual residential exposure (<0.2 µT). The mean HOME and BED personal exposure above transformers was 0.825 and 1.033 µT, respectively. Our study provides exposure assessment of a cohort with a wider exposure range, compared to power‐line epidemiological studies. Bioelectromagnetics. © 2006 Wiley‐Liss, Inc.
The effect of induced biphasic pulsed currents on re‐epithelialization of a novel wound healing model
Authors: Bullock Anthony J. , Barker Anthony T. , Coulton Leslie , MacNeil Sheila 
Published: September 2006

AbstractThe coordinated migration of keratinocytes is crucial to cutaneous wound healing; failure of keratinocytes to migrate into a wound can lead to chronic non‐healing wounds. Keratinocyte migration can be influenced by applied electrical fields. Our aim was to investigate whether keratinocyte migration could be accelerated by applying an induced biphasic pulsed electrical field. We developed two in vitro biological systems models for this purpose: a keratinocyte colony‐forming model and a reconstituted skin wound healing model with biphasic pulsed currents. Our in vitro skin models were capable of generating trans‐epithelial potentials (TEP) similar to in vivo mammalian skin. Histological examination of the wound healing model also indicated that re‐epithelialization occurred in a similar manner to that seen in vivo, although no evidence of a reconstitution of a basement membrane was seen during the 14 days in vitro experimental period. We found that growth of keratinocyte colonies and keratinocyte migration in an in vitro wound bed were not significantly affected by induced short duration biphasic pulsed currents at a frequency of 0.5 Hz of 100 and 200 mV/mm. Bioelectromagnetics. © 2006 Wiley‐Liss, Inc.
Magnetic field exposure and prognostic factors in childhood leukemia
Authors: Foliart Donna E. , Mezei Gabor , Iriye Richard , Silva J. Michael , Ebi Kristie L. , Kheifets Leeka , Link Michael P. , Kavet Rob , Pollock Brad H. 
Published: September 2006

AbstractWe examined the association between magnetic field (MF) exposure and the presence of prognostic risk factors among 482 children diagnosed with acute lymphoblastic leukemia (ALL) between 1996 and 2001. Personal 24‐h MF measurements were obtained for 412 children; 386 children were included in analyses. There were no trends seen between increasing exposure to MF and the presence of adverse clinical and tumor‐specific prognostic factors. Our results suggest that exposure to MF is not associated with the presence of unfavorable cytogenetic abnormalities in leukemic blast cells or with clinical factors at the time of diagnosis that predict poor survival. Bioelectromagnetics. © 2006 Wiley‐Liss, Inc.
Gibbs Free Energy of Formation of the Ternary Oxide Nd3RuO7
Authors: Banerjee Aparna , Prasad R. , Vaidya V. N. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Thermodynamic Calculation of LiH↔Li3AlH6↔LiAlH4 Reactions.
Authors: Jang Je‐Wook , Shim Jae‐Hyeok , Cho Young Whan , Lee Byeong‐Joo 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
On the Phase Relations in the ZrO2—YO1.5—AlO1.5 System
Authors: Lakiza S. , Fabrichnaya O. , Zinkevich M. , Aldinger F. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Experimental Evidence for Linear Metal—Azido Coordination: The Binary Group 5 Azides [Nb(N3)5], [Ta(N3)5], [Nb(N3)6]‐, and [Ta(N3)6]‐, and 1:1 Acetonitrile Adducts [Nb(N3)5(CH3CN)] and [Ta(N3)5(CH3CN)].
Authors: Haiges Ralf , Boatz Jerry A. , Schroer Thorsten , Yousufuddin Muhammed , Christe Karl O. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Synthesis of Oxadiazoloquinoxaline, Oxathiadiazoloquinoxaline and Oxadiazolobenzothiazine Derivatives.
Authors: Fylaktakidou Konstantina C. , Litinas Konstantinos E. , Saragliadis Athanasios , Adamopoulos Spyros G. , Nicolaides Demetrios N. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Chloroformamidinium Salts: Efficient Reagents for Preparation of 2‐Aminobenzoimidazole, 2‐Aminobenzoxazole, and 2‐Aminobenzothiazole Derivatives.
Authors: El‐Faham Ayman , Chebbo Mohamed , Abdul‐Ghani Mohamed , Younes Ghassan 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
1,2‐Dioximes in the Trofimov Reaction.
Authors: Zaitsev A. B. , Schmidt E. Yu. , Vasil'tsov A. M. , Mikhaleva A. I. , Petrova O. V. , Afonin A. V. , Zorina N. V. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Facile Aerobic Photooxidation of Alcohols in the Presence of Catalytic N‐Bromosuccinimide.
Authors: Kuwabara Kiyoto , Itoh Akichika 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
The Selective Solid‐Phase Oxidation of Alcohols and Other Organic Substrates by 3,5‐Dimethylpyrazolium Fluorochromate.
Authors: Chaudhuri M. K. , Dehury S. K. , Hussain S. , Duarah A. , Gogoi N. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Highly Regioselective Pd‐Catalyzed Intermolecular Aminoacetoxylation of Alkenes and Evidence for cis‐Aminopalladation and SN2 C—O Bond Formation.
Authors: Liu Guosheng , Stahl Shannon S. 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Zinc‐Mediated Allylation Reactions of Aldehydes and Ketones in Aqueous Media under Ultrasonic Irradiation.
Authors: Bian Yan‐Jiang , Fan Chun‐Ran , Hu Xiao‐Hong , Li Ji‐Tai 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Ytterbium Triflate Catalyzed Synthesis of β‐Keto Enol Ethers.
Authors: Curini Massimo , Epifano Francesco , Genovese Salvatore 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Novel and Effective Synthesis of 2‐Acyloxy‐3‐keto Esters.
Authors: Komiyama Takuzo , Takaguchi Yutaka , Tsuboi Sadao 
Published: September 2006

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.