bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Cross-Protective Immunity is Elicited by Infection with Contemporary Influenza B Lineage Viruses
Authors: Page Caroline , Shepard Justin D. , Ray Sean D. , Akinpelu Jasmine Y. , Geiger Ginger , Tompkins Stephen M. 
Published: April 2024

AbstractInfluenza B virus (FLUBV) significantly contributes to the influenza disease burden and has complicated vaccine development and efficacy, yet remains understudied compared to its counterpart, influenza A virus (FLUAV). Since its isolation in 1940, FLUBV has diverged into two antigenically distinct lineages: Victoria (B/Vic) and Yamagata (B/Yam). Recent human studies and epidemiological modeling reveal differences in immunity elicited by each FLUBV lineage, contributing to higher reinfection rates following B/Yam infection. To investigate disparities in FLUBV lineage cross-protection and immunity, we examined the effects of lineage-specific prior immunity on FLUBV reinfection dynamics. Mice were infected with representative B/Vic and B/Yam viruses from evolutionary distinct clades and subsequently reinfected with heterolineal viruses (i.e., B/Vic → B/Yam and B/Yam → B/Vic) to assess the extent of protection elicited between the lineages. Using this validated challenge model, we explored potential mechanisms underlying the asymmetric reinfection dynamics observed between the lineages. Our findings align with human observations, indicating that contemporary B/Vic viruses confer cross-protection against contemporary B/Yam infections, whereas contemporary B/Yam viruses do not provide the same degree of protection. Furthermore, we demonstrated that serum antibodies elicited by hemagglutinin vaccination cannot account for the observed heterolineal protection. Rather, antibodies targeting the viral neuraminidase (NA) may play a significant role in eliciting cross-protection to subsequent FLUBV infection. Our findings define asymmetric cross-protection resulting from contemporary FLUBV infection and suggest NA as a potential significant contributor to heterolineal FLUBV protection. This asymmetric immunity may also help explain the proposed extinction of B/Yam viruses since the COVID-19 pandemic.ImportanceInfluenza B viruses (FLUBV) consist of two divergently evolving lineages, Victoria (B/Vic) and Yamagata (B/Yam). Contemporary isolates from these lineages exhibit increased endemic activity and higher evolutionary rates while utilizing distinct mechanisms for evolutionary success. This is exemplified by novel seasonal infection dynamics with Influenza A viruses, differences in cross-protection elicited between the FLUBV lineages, and the potential extinction of B/Yam following the COVID-19 pandemic. We explore FLUBV infection dynamics utilizing contemporary viruses to define the asymmetric immunity elicited between the lineages. Contemporary Yamagata viruses are unable to confer the same breadth of protection as Victoria viruses. This may help explain the higher reinfection rates for Yamagata viruses and suggest a potential contributor to the extinction of this lineage.
Co-existence of plasmid-mediatedblaNDM-1andblaNDM-5inEscherichia colisequence type 167 and ST101 and their discrimination through restriction digestion
Authors: Bhattacharjee Amrita , Basak Priyanka , Mitra Shravani , Sarkar Jagannath , Dutta Shanta , Basu Sulagna 
Published: April 2024

AbstractThe concurrent presence of multiple New Delhi metallo-β-lactamase (blaNDM) variants within an isolate often goes undetected without the use of next-generation sequencing. This study detects and characterizes dualblaNDM-variants inEscherichia colithrough Sanger and whole-genome sequencing. Additionally, a rapid identification method utilizing restriction digestion was designed for detecting variants carrying M154L mutation. Antibiotic susceptibility, minimal inhibitory concentration for meropenem and ertapenem, PCR and Sanger sequencing ofblaNDMalong with genome sequencing using Ilumina and Nanopore technology were conducted. Transmissibility and replicon types ofblaNDM-harbouring plasmids were evaluated. Restriction digestion using restriction enzyme, BtsCI was developed to distinguish betweenblaNDM-1and variants possessing M154L mutations. Two isolates belonging to phylogroups A; ST167 and B1; ST101 and resistant to meropenem and ertapenem (≥16mg/L) were recovered from the blood of a neonate and the rectal swab of a pregnant woman respectively.blaNDMwas detected by PCR, and Sanger sequences ofblaNDMshowed two peaks at 262 (G & T) and 460 (A & C) nucleotide positions indicative of more than oneblaNDMvariant. Hybrid assembly confirmed co-existence ofblaNDM-1andblaNDM-5in each isolate.blaNDM-1was located on IncY (ST167) and IncHI1A/HI1B (ST101), whileblaNDM-5was on a IncFIA/FII (ST167) and IncC (ST101) plasmids. Digestion with BtsC1 could discriminateblaNDM-1andblaNDM-5. Co-existence of multipleblaNDMS,blaNDM-1andblaNDM-5in epidemic clones ofE. coliis concerning. Restriction digestion method and Sanger sequencing can facilitate quick identification of dualblaNDMvariants in single isolate.ImportanceThe global dissemination of antimicrobial resistance genes is a serious concern. One such gene,blaNDM, has spread all across the globe via plasmids.blaNDMconfers resistance against all β-lactam antibiotics except monobactams. Most of the earlier literature reported presence of singleblaNDMvariants. However, this study reports the prevalence of dualblaNDMvariants (blaNDM-1andblaNDM-5) located on two separate plasmids identified in two distinctE. coliepidemic clones ST167 and ST101; isolated from a septicaemic neonate and a pregnant mother respectively.blaNDM-5differs fromblaNDM-1due to presence of two point mutations i.e., V88L and M154L. This study detected dualblaNDM-variants through Sanger sequences and further validated through hybrid-genome assembly. Detection of multipleblaNDM-variants in a single isolate remains difficult until genome sequencing or southern blotting are carried out. Hence, a simple restriction digestion method was devised for rapid screening of dualblaNDM-variants containing M154L mutation.
The Proximal Centriole-Like Structure Anchors the Centriole to the Sperm Nucleus
Authors: Buglak Danielle B. , Holmes Kathleen H.M. , Galletta Brian J. , Rusan Nasser M. 
Published: April 2024

ABSTRACTProper connection between the sperm head and tail is critical for sperm motility and fertilization. The link between the head and tail is mediated by the Head-Tail Coupling Apparatus (HTCA), which secures the axoneme (tail) to the nucleus (head). However, the molecular architecture of the HTCA is not well understood. Here, we useDrosophilato create a high-resolution map of proteins and structures at the HTCA throughout spermiogenesis. Using structured illumination microscopy, we demonstrate that key HTCA proteins Spag4 and Yuri form a ‘Centriole Cap’ that surrounds the centriole (or Basal Body) as it is inserted, or embedded into the surface of the nucleus. As development progresses, the centriole is laterally displaces to the side of the nucleus, during which time the HTCA expands under the nucleus, forming what we term the ‘Nuclear Shelf.’ We next show that the proximal centriole-like (PCL) structure is positioned under the Nuclear Shelf and functions as a critical stabilizer of the centriole-nuclear attachment. Together, our data indicate that the HTCA is complex, multi-point attachment site that simultaneously engages the PCL, the centriole, and the nucleus to ensure proper head-tail connection during late-stage spermiogenesis.
Synthetic G protein-coupled receptors for programmable sensing and control of cell behavior
Authors: Kalogriopoulos Nicholas A. , Tei Reika , Yan Yuqi , Ravalin Matthew , Li Yulong , Ting Alice 
Published: April 2024

AbstractSynthetic receptors that mediate antigen-dependent cell responses are transforming therapeutics, drug discovery, and basic research. However, established technologies such as chimeric antigen receptors (CARs) can only detect immobilized antigens, have limited output scope, and lack built-in drug control. Here, we engineer synthetic G protein-coupled receptors (GPCRs) capable of driving a wide range of native or nonnative cellular processes in response to user-defined antigen. We achieve modular antigen gating by engineering and fusing a conditional auto-inhibitory domain onto GPCR scaffolds. Antigen binding to a fused nanobody relieves auto-inhibition and enables receptor activation by drug, thus generating Programmable Antigen-gated G protein-coupled Engineered Receptors (PAGERs). We create PAGERs responsive to more than a dozen biologically and therapeutically important soluble and cell surface antigens, in a single step, from corresponding nanobody binders. Different PAGER scaffolds permit antigen binding to drive transgene expression, real-time fluorescence, or endogenous G protein activation, enabling control of cytosolic Ca2+, lipid signaling, cAMP, and neuronal activity. Due to its modular design and generalizability, we expect PAGER to have broad utility in discovery and translational science.
Bacteriophage Φ21’s receptor-binding protein evolves new functions through destabilizing mutations that generate non-genetic phenotypic heterogeneity
Authors: Gerbino Krista R. , Borin Joshua M. , Ardell Sarah M. , Lee Justin J. , Corbett Kevin D. , Meyer Justin R. 
Published: April 2024

AbstractHow viruses evolve to expand their host range is a major question with implications for predicting the next pandemic. Gain-of-function experiments have revealed that host-range expansions can occur through relatively few mutations in viral receptor-binding proteins, and the search for molecular mechanisms that explain such expansions are underway. Previous research on expansions of receptor use in bacteriophage λ has shown that mutations that destabilize λ’s receptor-binding protein cause the receptor-binding protein to fold into new conformations that can utilize novel receptors but have weakened thermostability. These observations led us to hypothesize that other viruses may take similar paths to expand their host range. Here, we find support for our hypothesis by studying another virus, bacteriophage 21 (Φ21), which evolves to use two new host receptors within two weeks of laboratory evolution. By measuring the thermodynamic stability of Φ21 and its descendants, we show that, as Φ21 evolves to use new receptors and expands its host range, it becomes less stable and produces viral particles that are genetically identical but vary in their thermostabilities. Next, we show that this non-genetic heterogeneity between particles is directly associated with receptor use innovation, as phage particles with more derived receptor use capabilities are more unstable and decay faster. Lastly, by manipulating the expression of protein chaperones during Φ21 infection, we demonstrate that heterogeneity in thermostability and receptor use breadth of phage particles is directly related to the folding of phage proteins into different conformers. Altogether, our results provide support for the hypothesis that viruses can evolve new receptor-use tropisms through mutations that destabilize the receptor-binding protein and produce multiple protein conformers.
Plasmon dispersion in bilayer cuprate superconductors
Authors: Bejas M. , Zimmermann V. , Betto D. , Boyko T. D. , Green R. J. , Loew T. , Brookes N. B. , Cristiani G. , Logvenov G. , Minola M. , Keimer B. , Yamase H. , Greco A. , Hepting M. 
Published: April 2024

The essential building blocks of cuprate superconductors are two-dimensional CuO2 sheets interspersed with charge reservoir layers. In bilayer cuprates, two closely spaced CuO2 sheets are separated by a larger distance from the subsequent pair in the next unit cell. In contrast to single-layer cuprates, prior theoretical work on bilayer systems has predicted two distinct acoustic plasmon bands for a given out-of-plane momentum transfer. Here we report random phase approximation (RPA) calculations for bilayer systems which corroborate the existence of two distinct plasmon bands. We find that the intensity of the lower-energy band is negligibly small in most parts of the Brillouin zone, whereas the higher-energy band carries significant spectral weight. We also present resonant inelastic x-ray scattering (RIXS) experiments at the O K-edge on the bilayer cuprate Y0.85Ca0.15Ba2Cu3O7 (Ca-YBCO), which show only one dispersive plasmon branch, in agreement with the RPA calculations. In addition, the RPA results indicate that the dispersion of the higher-energy plasmon band in Ca-YBCO is not strictly acoustic but exhibits a substantial energy gap of approximately 250 meV at the two-dimensional Brillouin zone center.

Published by the American Physical Society
2024
About the magnitude of the
γ*N→N(1520)
transverse amplitudes near
Q2=0
Authors: Ramalho G. 
Published: April 2024

The γ*N→N(1520) transition has a property that differs from the other low-lying nucleon resonance amplitudes: the magnitude of the transverse helicity amplitudes. The transition helicity amplitudes are defined in terms of square-transfer momentum q2, or Q2=−q2. Near the photon point (Q2=0) there is a significant difference in the magnitude of the transverse amplitudes: A3/2 is very large and A1/2 is very small. This atypical behavior contrasts with the relation between the amplitudes at the pseudothreshold [the limit where the nucleon and the N(1520) are both at rest and Q2<0], where A3/2=A1/2/3, and also in the large-Q2 region, where theory and data suggest that A3/2 is suppressed relative to A1/2. In the present work, we look for the source of the suppression of the A1/2 amplitude at Q2=0. The result is easy to understand in first approximation, when we look into the relation between the transverse amplitudes and the elementary form factors, defined by a gauge-invariant parametrization of the γ*N→N(1520) transition current, near Q2=0. There is a partial cancellation between contributions of two elementary form factors near Q2=0. We conclude, however, that the correlation between the two elementary form factors at Q2=0 is not sufficient to explain the transverse amplitude data below Q2=1  GeV2. The description of the dependence of the transverse amplitudes on Q2 requires the determination of the scale of variation of the elementary form factors in the range Q2=0…0.5  GeV2, a region with almost nonexistent data. We conclude at the end that the low-Q2 data for the transverse amplitudes can be well described when we relate the scale of variation of the elementary form factors with the nucleon dipole form factor.

Published by the American Physical Society
2024
Exploring perspectives on living through the COVID-19 pandemic for people experiencing homelessness and dealing with mental ill-health and/or substance use: qualitative study
Authors: Adams Emma Audrey , Hunter Desmond , Kennedy Joanne , Jablonski Tony , Parker Jeff , Tasker Fiona , Widnall Emily , O'Donnell Amy Jane , Kaner Eileen , Ramsay Sheena E. 
Published: April 2024

Purpose
This study aims to explore the experiences of living through the COVID-19 pandemic for people who faced homelessness and dealt with mental health and/or substance use challenges.

Design/methodology/approach
This qualitative study was comprised of 26 1:1 interviews (16 men and 10 women), conducted between February and May 2021 with people who experienced homelessness in North East England during the COVID-19 pandemic. An inductive reflexive thematic analysis was undertaken, with input from individuals with lived experience who were involved throughout the study.

Findings
Four themes were developed. The first theme, lack of support and exacerbation of mental health and substance use difficulties, highlighted how the lack of in-person support and increased isolation and loneliness led to relapses or new challenges for many people’s mental health and substance use. The second theme, uncertainty and fear during the pandemic, explored how the “surreal” experience of the pandemic led to many people feeling uncertain about the future and when things would return to normal. The third theme, isolation and impacts on social networks, discussed how isolation and changes to relationships also played a role in mental health and substance use. Finally, opportunity for reflection and self-improvement for mental health and substance use, explored how some people used the isolated time to re-evaluate their recovery journey and focus on self-improvement.

Practical implications
The experiences shared within this study have important implications for planning the future delivery and commissioning of health and social care services for people facing homelessness, such as sharing information accessibly through clear, consistent and simple language.

Originality/value
As one of the few papers to involve people with lived experience as part of the research, the findings reflect the unique narratives of this population with a focus on improving services.
Potential errors during final inspection and certification process of an aircraft component
Authors: Abbas Jibran , Khare Ashish 
Published: April 2024

Purpose
According to regulations, aircraft must be in an airworthy condition before they can be operated. To ensure airworthiness, they must be maintained by an approved component maintenance organisation. This study is aimed to identify potential errors that may arise during the final inspection and certification process of aircraft components, categorise them, determine their consequences and quantify the associated risks. Any removed aircraft components must be sent to an approved aircraft component maintenance organisation for further maintenance and issuance of European Union Aviation Safety Agency (EASA) Form 1. Thereafter, a final inspection and certification process must be conducted by certifying staff to receive an EASA Form 1. This process is crucial because any errors during this stage can result in the installation of unsafe components in an aircraft.

Design/methodology/approach
The Systematic Human Error Reduction and Prediction Approach (SHERPA) method was used to identify potential errors. This method involved a review of the procedures of three maintenance organisations, individual interviews with ten subject matter experts and a consensus group of 14 certifying staff from different maintenance organisations to achieve the desired results.

Findings
In this study, 39 potential errors were identified during the final inspection and certification process. Furthermore, analysis revealed that 48.7% of these issues were attributed to checking errors, making it the most common type of error observed.

Originality/value
This study pinpoints the potential errors in the final inspection and certification of aircraft components. It offers maintenance organisations a roadmap to assess procedures, implement preventive measures and reduce the likelihood of these errors.
Examining the effects of fear of missing out on sustainable food consumption: a social identity perspective
Authors: Singh Suruchi , Banerjee Shubhomoy 
Published: April 2024

PurposeThis study employs the Social Identity Theory to examine the differential effects of personal and social dimensions of fear of missing out (FOMO) on sustainable food consumption (SFC) practices.Design/methodology/approachAn online survey-based empirical study was conducted with 395 respondents. The data were analysed using structural equation modelling and Hayes process Macro in SPSS.FindingsSFC was found to be positively influenced by personal FOMO. Contrary to expectations, social FOMO had a negative correlation with SFC. Social influence and social identity were shown to be positively correlated, whilst the social influence-SFC relationship was favourable. This approach was aided by social identity.Research limitations/implicationsThe study supports personal FOMO as an SFC-influencing factor. It evaluates the differential effects of FOMO’s personal and social dimensions on SFC. It also demonstrates that social FOMO negatively affects SFC, contrary to expectations.Practical implicationsThe study advises sustainable food firms to reduce personal FOMO via advertising and messaging.Originality/valueThis research is amongst the first to segregate the differential effects of social and personal FOMO regarding SFC behaviour. Research has examined FOMO as a higher-order construct involving social and personal aspects. Second, FOMO is often associated with negative behaviours including social media addiction and substance abuse. This FOMO-related research analyses a desired behaviour.
Decreasing employability with age? The role of automation risk, lifelong learning and occupational mobility
Authors: Goštautaitė Bernadeta , Šerelytė Miglė 
Published: April 2024

PurposeAs aging populations lead to longer working lives and increasing automation threatens job security, maintaining lifelong employability is becoming a fundamental challenge for many individuals. The purpose of this study is to examine how lifelong employability can be maintained. Design/methodology/approachBased on the theoretical perspectives of both movement capital and selection, optimization and compensation (SOC) theories, we used large-scale survey data (N = 2,256) from three European countries to investigate strategies for preserving employability among aging workers. Specifically, we explored the perceived risk of automation, lifelong learner characteristics and self-efficacy for occupational mobility as boundary conditions that may shape the negative relationship between age and employability.FindingsWe found a negative relationship between age and employability, which was more pronounced when the perceived risk of automation was higher. Furthermore, lifelong learner characteristics and self-efficacy for occupational mobility mitigated the negative relationship, so that age was not related to employability if people possessed lifelong learner characteristics and were ready for a career change.Originality/valueOur study implies the importance of investing in enhancing lifelong learner characteristics and self-efficacy for occupational mobility for older employees.
The role of attachment to K-celebrity from a destination marketing perspective
Authors: Kim MiRan , Lee Heijin , Kim Soyeon , Choi Laee 
Published: April 2024

Purpose
Although there is a growing body of literature on how celebrity involvement impacts the effectiveness of destination marketing, the underlying mechanisms of that relationship are still underexplored. Based on the affect transfer and meaning transfer theories, this study aims to examine the impact of celebrity attachment on customer delight toward K-culture and K-culture attachment, affective and cognitive images of Korea, and the intention to visit Korea.

Design/methodology/approach
Online survey data were collected from 2,614 US residents, representing various demographic characteristics. For the data analysis, the partial least squares-structural equation modeling was conducted to evaluate the structural model and test the hypotheses.

Findings
The results showed that celebrity attachment is positively related to customer delight toward K-culture and K-culture attachment, which, in turn, positively influences affective and cognitive images of Korea. Additionally, K-culture attachment positively influences cognitive and affective images of Korea, which are positively related to the intention to visit Korea.

Research limitations/implications
By using the affect transfer theory and meaning transfer theory, this study provides valuable insights into how consumer’s attachment to celebrities has spillover effects on the decision-making process. This study also adds a new concept, customer delight connected to cultural experience, in the context of destination marketing.

Practical implications
By understanding the importance and influence of people’s intimacy with media characters, practitioners can apply parasocial relationship theory, affect transfer theory and meaning transfer theory to their marketing strategies.

Originality/value
As one of the few empirical studies that examines the impact of celebrity attachment on consumers’ perceptions and behaviors, this study can make significant contributions to the destination marketing literature.