bluuebunny/crossref_metadata_2025_split huggingface dataset:
Karakteristik penderita Lepra (Kusta) yang menjalani pengobatan rawat jalan di Puskesmas Tamalate Makassar periode 2018–2021
Authors: Amaliah H.R Riski , Lisa Yuniati  , Roem Nurul Rumila , Sri Vitayani  , Solecha Setiawati  
Published: March 2024

Kusta adalah penyakit menular karena Mycobacterium leprae. Kusta adalah penyakit menular yang tetap endemik di banyak wilayah di dunia, Brasil, India dan Indonesia menyumbang sekitar 80% dari kasus baru yang terdaftar secara global. Indonesia berada di peringkat ketiga di dunia setelah India dan Brazil, dengan jumlah Penderita Kusta baru pada tahun 2017 mencapai 15.910 Penderita Kusta (angka penemuan Penderita Kusta baru 6,07 per 100.000 penduduk). Ada beberapa hal yang menjadi distribusi Penderita Kusta menurut faktor manusia, antara lain: usia, jenis kelamin, etnik dan suku dan faktor sosial ekonomi. Tujuan penelitian ini adalah untuk mengetahui karakteristik penderita Lepra (Kusta) yang menjalani pengobatan rawat jalan di puskesmas Tamalate Makassar periode 2018–2021. Penelitian ini adalah penelitian dengan metode penelitian deskriptif dengan pendekatan cross-sectional dengan menggunakan data sekunder yaitu rekam medik pasien lepra (kusta) dan pengambilannya dengan cara teknik total sampling. Ditemukan distribusi penderita lepra atau kusta yang menjalani perawatan rawat jalan di Puskesmas Tamalate Makassar periode 2018-2021 berdasarkan klasifikasinya terbanyak pada kusta tipe Multibasiller yaitu 33 orang (78%), berdasarkan usia didapatkan terbanyak pada kelompok usia 26-35 tahun (28,6%), berdasarkan jenis kelamin didapatkan lebih banyak pada kelompok laki-laki sebanyak 34 orang (81%), dan berdasarkan pekerjaan lebih banyak pada pasien yang bekerja sebagai buru harian yaitu 16 orang (38,1%). Kesimpulan Karakteristik penderita Lepra (Kusta) yang menjalani pengobatan rawat jalan di puskesmas Tamalate Makassar periode 2018–2021 yaitu terbanyak pada kusta tipe Multibasiller, kelompok usia 26-35 tahun, berjenis kelamin laki-laki dan pasien yang bekerja sebagai buruh harian.
Gambaran KarakteristikPasien Otitis Eksterna di RSUD Haji Makassar PeriodeJanuari-Desember 2021
Authors: Hamzah Miftahul Jannah 
Published: March 2024

Pendahuluan: Indonesia merupakan negara dengan iklim tropis. Indonesia sendiri merupakan bagian dari iklim tropis lembab atau zona lembab dan panas, dimana ditetapkan kriteria faktor predisposisi terjadinya otitis eksterna. Otitis eksterna merupakan penyakit radang telinga yang terbagi menjadi akut dan kronis yang disebabkan oleh bakteri, jamur dan virus. Tujuan penelitian: Mengetahui gambaran tipikal otitis media rawat jalan di Rumah Sakit Haji Massari antara Januari dan Desember 2021. Metode: Desain penelitian yang digunakan dalam penelitian ini adalah deskriptif dan retrospektif, dan bahan penelitian berupa rekam medis rawat jalan otitis media. Hasil penelitian: Hasil penelitian ini menunjukkan bahwa penderita otitis eksterna terbanyak pada kelompok jenis kelamin, dengan mayoritas berjenis kelamin perempuan (56%) dan terbanyak pada kelompok usia 17-25 dan 36-45 tahun (19,5%); paling sakit atau sakit telinga (57,1%); dan otitis eksterna yang paling banyak ditemukan adalah otitis eksterna akut difus (88,7%). Kesimpulan: Dari penelitian ini dapat disimpulkan bahwa otitis eksterna dapat terjadi pada usia berapapun, tidak dipengaruhi oleh jenis kelamin atau hormon, dan biasanya muncul sebagai gejala utama berupa nyeri telinga.
Kata kunci: Karakteristik; otitis eksterna; RSUD Haji Makassar
Risk Factors for Cervical Cancer Incidence at Ibnu Sina Hospital Makassar
Authors: Khairun Nadhifah 
Published: March 2024

Cervical cancer is a malignant tumor that occurs in the cervical region at the end of the cervix. Based on data from the International Agency for Research on Cancer (IARC), it is known that the second highest cause of death is cervical cancer, reaching 460,000 cases and 230,000 women deaths. Cervical cancer is caused by HPV infection and several factors, namely age, parity, age of first sexual intercourse, number of sexual partners history, HPV vaccination history, smoking history, and oral contraceptives history. To determine the risk factors for cervical cancer incidence at Ibnu Sina Hospital Makassar. This was descriptive retrospective study method. With cross sectional research design. The total sample was 68 people with cervical cancer at Ibnu Sina Hospital Makassar. The distribution of age 30-60 years (85.3%), parity >3 (57.4%), and age of first sexual intercourse >17 years (85.3%). There was no history of using oral contraceptives (54.4%), HPV vaccination history (97.1%), and smoking history (98.5%). Having only 1 history of number of sexual partners (89.7%), employment history was not out of town (63.2%), and the most common type of cervical cancer was squamous cell carcinoma (76.5%). The distribution of risk factors for the incidence of Cervical Cancer at Ibnu Sina Hospital was mostly found at the age of 30-60 years, parity> 3, age of first sexual intercourse >17 years, no history of using oral contraceptives, no HPV vaccination history, no smoking history, having 1 history of sexual partners, work history not out of town, and the type of cervical cancer is squamous cell carcinoma.
HUBUNGAN KEKUATAN OTOT LENGAN TERHADAP HASIL TOLAK PLURU GAYA OBRAIN SISWA KELAS IX SMPN 03 SELUMA
Authors: Rosa Elvina , Dewi Citra , Apriansyah Dolly 
Published: March 2024

This study aims to determine the relationship between the strength of the arm muscles and the results of the obrain style shot put for class IX students of SMPN 3 SELUMA. This type of research is descriptive with correlational analysis by looking for relationships between variables and data collection techniques using tests and measurements with a population and sample of 12 students. The results of this study based on data analysis obtained a p-value (sig) 0.000 <0.05. Based on the results above, it can be concluded that there is a significant (significant) relationship between arm muscle strength and obrain-style bullet repulsion in class IX students of SMPN 3 SELUMA.

Keywords: Relationship, Arm Muscle Strength, Shot Reject
Upaya Meningkatkan Hasil Belajar Lay Up Shoot Bola Basket Melalui Media Youtube Tutorial Teman Sebaya Pada Siswa Smpn 5 Kota Bengkulu
Authors: A Rahman , Mesterjon Mesterjon , Perdima F E 
Published: March 2024

Untuk mengetahui sejauh mana peningkatan hasil belajar Lay Up Shoot bola basket melalui media Youtube Tutorial teman sebaya pada Siswa SMPN 5 Kota Bengkulu. Desain penelitian ini adalah penelitian tindakan kelas dengan prosedur penelitian melalui; perencanaan, tindakan, observasi, dan refleksi. Subjek penelitian ini adalah siswa kelas X A SMPN 5 Kota Bengkulu yang berjumlah 36 siswa. Instrumen penelitian yang digunakan yaitu tes lay up shoot dan lembar observasi. Hasil penelitian menunjukkan bahwa media youtube tutorial teman sebaya sangat efektif dalam meningkatkan hasil belajar lay up shoot pada permainan bola basket di SMPN 5 Kota Bengkulu. Peningkatan hasil belajar lay up shoot pada permainan bola basket menggunakan media youtube tutorial teman sebaya yaitu dengan nilai rata-rata 70,27 di siklus I meningkat menjadi 80,55 di siklus II. 2) Selain meningkatkan kualitas pembelajaran, metode tutorial teman sebaya direkomendasikan untuk membantu guru dalam menumbuhkan kegembiraan dan kesenangan pada siswa dalam situasi kompetitif serta mampu memotivasi siswa dalam belajar.
Genomic insights into the evolution, pathogenicity, and extensively drug-resistance of emerging pathogens Kluyvera and Phytobacter
Authors: Huang Zhenzhou , Zhang Guozhong , Zheng Zhibei , Lou Xiuqin , Cao Feifei , Zeng Lingyi , Wang Duochun , Yu Keyi , Li Jun 
Published: March 2024

IntroductionKluyvera is a Gram-negative, flagellated, motile bacillus within the Enterobacteriaceae. The case reports of clinical infections shed light on the importance of this organism as an emerging opportunistic pathogen. The genus Phytobacter, which often be misidentified with Kluyvera, is also an important clinically relevant member of the Enterobacteriaceae. However, the identification of Kluyvera and Phytobacter is problematic, and their phylogenetic relationship remains unclear.MethodsHere, 81 strains of Kluyvera and 16 strains of Phytobacter were collected. A series of comparative genomics approaches were applied to the phylogenetic relationship reconstruction, virulence related genes profiles description, and antibiotic resistance genes prediction.ResultsUsing average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH), we offered reliable species designations of 97 strains, in which 40 (41.24%) strains were incorrectly labeled. A new Phytobacter genomospecies-1 were defined. Phytobacter and Kluyvera show great genome plasticity and inclusiveness, which may be related to their diverse ecological niches. An intergenomic distances threshold of 0.15875 was used for taxonomy reassignments at the phylogenomic-group level. Further principal coordinates analysis (PCoA) revealed 11 core genes of Kluyvera (pelX, mdtL, bglC, pcak-1, uhpB, ddpA-2, pdxY, oppD-1, cptA, yidZ, csbX) that could be served as potential identification targets. Meanwhile, the Phytobacter specific virulence genes clbS, csgA-C, fliS, hsiB1_vipA and hsiC1_vipB, were found to differentiate from Kluyvera. We concluded that the evolution rate of Kluyvera was 5.25E-6, approximately three times higher than that of Phytobacter. Additionally, the co-existence of ESBLs and carbapenem resistance genes were present in approximately 40% strains, suggesting the potential development of extensively drug-resistant or even fully drug-resistant strains.DiscussionThis work provided a better understanding of the differences between closely related species Kluyvera and Phytobacter. Their genomes exhibited great genome plasticity and inclusiveness. They not only possess a potential pathogenicity threat, but also a risk of multi-drug resistance. The emerging pathogens Kluyvera and Phytobacter warrant close attention.
Evaluation of left ventricular flow field changes after stress in patients with nonobstructive coronary artery disease using ultrasonic flow vector imaging
Authors: Li Dongmei , Zhao Xin , Xiao Qiuyu , Yang Rui , Li Zizhuo , Xie Yuanyuan , Mao Xinyue , Li Xi , Hu Wenhan , Deng Yan 
Published: March 2024

PurposeVector flow mapping and treadmill exercise stress echocardiography were used to evaluate and explore changes in the left ventricular (LV) flow field of patients with nonobstructive coronary artery disease.MethodsOverall, 34 patients with nonobstructive (&lt;50%) left anterior descending coronary artery stenosis (case group) and 36 patients with no coronary artery stenosis (control group) were included. Apical four-, three-, and two-chamber echocardiographic images were collected at rest and during early recovery from treadmill exercise. LV flow field, vortex area, and circulation (cir) changes were recorded in different phases: isovolumetric systole (S1), rapid ejection (S2), slow ejection (S3), isovolumetric diastole (D1), rapid filling (D2), slow filling (D3), and atrial systole (D4). Intra- and inter-group differences were compared before and after exercise loading.ResultsThe control and case groups demonstrated regular trends of eddy current formation and dissipation at rest and under stress. Compared with the control group, the case group had irregular streamline distributions. Abnormal vortices formed in the S1 and D3 apical segments and D1 left ventricular middle segment in the resting group. Compared with the control group, the resting group had decreased left ventricular S1 vortex areas and increased S3 vortex areas. The post-stress D1 and D3 vortex areas and D1 and D2 cir increased. Compared with at rest, after stress, the control group had decreased S1, S3, D2, and D3 vortex areas; increased S2, D1, D3, and D4 cir; and decreased D2 cir. After stress, the case group had decreased S3 and D2 vortex areas, increased D1 vortex areas, and increased S2, D1, D3, and D4 cir (P all &lt; 0.001). Logistic regression and ROC curve analyses show that increased D1 vortex area after stress is an independent risk factor for stenosis in nonobstructive stenosis of coronary arteries (OR: 1.007, 95% CI: 1.005–1.010, P &lt; 0.05). A D1 vortex area cutoff value of 82.26 had an AUC, sensitivity, and specificity of 0.67, 0.655, and 0.726, respectively.ConclusionThe resting left ventricular flow field changed in patients with nonobstructive left anterior descending coronary artery stenosis. Both groups had more disordered left ventricular blood flow after stress. The increased D1 vortex area after stress is an independent risk factor for mild coronary stenosis and may contribute to the assessment of nonobstructive coronary stenosis. VFM combined with treadmill stress is useful in evaluating left ventricular flow field changes in patients with nonobstructive coronary artery disease, which is valuable in the early evaluation of coronary heart disease.
Aetiology of non-malaria acute febrile illness fever in children in rural Guinea-Bissau: a prospective cross-sectional investigation
Authors: Gutierrez Rui , Landa Mariana , Sambou Masse , Bassane Hubert , Dia Ndongo , Djalo Alfa Saliu , Domenichini Chiara , Fall Gamou , Faye Martin , Faye Ousmane , Fernandez-Garcia Maria-Dolores , Flevaud Laurence , Loko Jerlie , Mediannikov Oleg , Mize Valerie , Ndiaye Kader , Niang Mbayame , Raoult Didier , Rocaspana Merce , Villen Susana , Sall Amadou Alpha , Fenollar Florence 
Published: March 2024

BackgroundWith growing use of parasitological tests to detect malaria and decreasing incidence of the disease in Africa; it becomes necessary to increase the understanding of causes of non-malaria acute febrile illness (NMAFI) towards providing appropriate case management. This research investigates causes of NMAFI in pediatric out-patients in rural Guinea-Bissau.MethodsChildren 0–5 years presenting acute fever (≥38°) or history of fever, negative malaria rapid diagnostic test (mRDT) and no signs of specific disease were recruited at the out-patient clinic of 3 health facilities in Bafatá province during 54 consecutive weeks (dry and rainy season). Medical history was recorded and blood, nasopharyngeal, stool and urine samples were collected and tested for the presence of 38 different potential aetiological causes of fever.ResultsSamples from 741 children were analysed, the protocol was successful in determining a probable aetiological cause of acute fever in 544 (73.61%) cases. Respiratory viruses were the most frequently identified pathogens, present in the nasopharynx samples of 435 (58.86%) cases, followed by bacteria detected in 167 (22.60%) samples. Despite presenting negative mRDTs, P. falciparum was identified in samples of 24 (3.25%) patients.ConclusionsThis research provides a description of the aetiological causes of NMAFI in West African context. Evidence of viral infections were more commonly found than bacteria or parasites.
Stressing out—carp edema virus induces stress and modulates immune response in common carp
Authors: Zawisza Maria , Rebl Alexander , Teitge Felix , Krzystyniak Barbara , Piackova Veronika , Gela David , Kocour Martin , Chadzinska Magdalena , Adamek Mikolaj , Rakus Krzysztof 
Published: March 2024

IntroductionCarp edema virus (CEV) is a fish poxvirus that primarily infects the gills of common carp. CEV causes koi sleepy disease (KSD), which is highly contagious and can result in mortality of up to 100%.MethodsIn the present study, we analyzed the stress and immune responses during KSD in two strains of common carp with different resistance to CEV: susceptible koi and resistant Amur sazan. Experiments were performed at two temperatures: 12°C and 18°C. In the case of koi carp, we also analyzed the effect of supplementation of 0.6% NaCl into tank water, which prevents mortality of the CEV-infected fish (salt rescue model).ResultsWe found that CEV-infected koi kept at 18°C had the highest viral load, which correlated with the most severe histopathological changes in the gills. CEV infection resulted in the activation of stress response reflected by the upregulated expression of genes involved in stress response in the stress axis organs and increased levels of cortisol and glucose in the blood plasma. These changes were the most pronounced in CEV-infected koi kept at 18°C. At both temperatures, the activation of antiviral immune response was observed in koi kept under freshwater and NaCl conditions upon CEV infection. Interestingly, a clear downregulation of the expression of adaptive immune genes was observed in CEV-infected koi kept under freshwater at 18°C.ConclusionCEV induces a stress response and modulates adaptive immune response in koi, and this is correlated with the level of viral load and disease development.
Reduced serum IgG galactosylation is associated with increased inflammation during relapses of neuromyelitis optica spectrum disorders
Authors: Gao Shiyu , Jiao Xin , Guo Ruoyi , Song Xiujuan , Li Bin , Guo Li 
Published: March 2024

Background and ObjectivePost-translational modifications of antibodies, with a specific focus on galactosylation, have garnered increasing attention in the context of understanding the pathogenesis and therapeutic implications of autoimmune diseases. However, the comprehensive scope and the clinical significance of antibody galactosylation in the context of Neuromyelitis Optica Spectrum Disorder (NMOSD) remain enigmatic.The primary aim of this research was to discern disparities in serum IgG galactosylation levels between individuals in the acute stage of NMOSD relapse and their age- and sex-matched healthy counterparts.MethodsA total of fourteen untreated NMOSD patients experiencing an acute relapse phase, along with thirteen patients under medication, were enrolled, and an additional twelve healthy controls of the same age and gender were recruited for this investigation. Western blot and lectin enzyme techniques were used to determine the level of IgG galactosylation in the serum samples from these subjects. The expression of CD45+, CD3+, CD3+CD4+, CD3+CD8+, CD19+, and CD16+CD56+ in peripheral blood leukocytes was measured by flow cytometry. The enzyme-linked immunosorbent assay (ELISA) was also used to quantify the amounts of IgG. Magnetic particle luminescence assays are used to detect cytokines. Robust statistical analysis was executed to ascertain the potential associations between IgG galactosylation and the aforementioned immune indices.ResultsIn the context of NMOSD relapses, serum IgG galactosylation exhibited a notable decrease in untreated patients (0.2482 ± 0.0261), while it remained comparatively stable in medicated patients when contrasted with healthy controls (0.3625 ± 0.0259) (p=0.0159). Furthermore, a noteworthy inverse correlation between serum IgG galactosylation levels and the Expanded Disability Status Scale (EDSS) score during NMOSD relapse was observed (r=-0.4142; p=0.0317). Notably, IgG galactosylation displayed an inverse correlation with NMOSD relapse among peripheral blood CD45+, CD3+, CD3+CD8+, CD19+ cells, as well as with IL-6 and IL-8. Nevertheless, it was not determined whether IgG galactosylation and CD3+CD4+ T cells or other cytokines are statistically significantly correlated.ConclusionOur research identified reduced IgG galactosylation in the serum of NMOSD patients during relapses, significantly correlated with disease severity, thereby providing a novel target for the diagnosis and treatment of NMOSD in the realm of medical research.
Clinical and immunologic features of co-infection in COVID-19 patients, along with potential traditional Chinese medicine treatments
Authors: Zhang Guochao , Zhang Junjun , Gao Qi , Zhao Yingying , Lai Yanjun 
Published: March 2024

ObjectivesWith the increasing number of people worldwide infected with SARS-CoV-2, the likelihood of co-infection and/or comorbidities is rising. The impact of these co-infections on the patient’s immune system remains unclear. This study aims to investigate the immunological characteristics of secondary infections in hospitalized COVID-19 patients, and preliminarily predict potential therapeutic effects of traditional Chinese medicine and their derivatives for the treatment of co-infections.MethodsIn this retrospective cohort study, we included 131 hospitalized patients with laboratory-confirmed COVID-19, of whom there were 64 mild and 67 severe cases. We analyzed clinical characteristics and immunologic data, including circulating immune cell numbers, levels of inflammatory factors and viral load, comparing COVID-19 patients with and without co-infection.ResultsAmong 131 hospitalized COVID-19 patients, 41 (31.3%) were co-infection positive, with 33 (80.5%) having severe disease and 14 (34.1%) of them resulting in fatalities. Co-infected patients exhibited significantly higher severity and mortality rates compared to non-co-infected counterparts. Co-infected patients had significantly lower absolute counts of lymphocytes, total T lymphocytes, CD4+ T cells, CD8+ T cells and B lymphocytes, while levels of hs-CRP, PCT and IL-6 were significantly elevated compared to non-co-infected patients. Additionally, the viral load of co-infected patients was significantly higher than non-co-infected patients.ConclusionCo-infection emerges as a dangerous factor for COVID-19 patients, elevating the risk of severe pneumonia and mortality. Co-infection suppresses the host’s immune response by reducing the number of lymphocytes and increasing inflammation, thereby diminishing the antiviral and anti-infective effects of the immune system, which promotes the severity of the disease. Therefore, it is crucial to implement infection prevention measures to minimize the spread of co-infections among COVID-19 hospitalized patients. Additionally, changes in these biomarkers provide a theoretical basis for the effective treatment of co-infections with traditional Chinese medicine.
Enhancing soil health and strawberry disease resistance: the impact of calcium cyanamide treatment on soil microbiota and physicochemical properties
Authors: Du Ying-chun , Yuan Can-sheng , Song Yu-qi , Yang Ying , Zheng Qing-song , Hou Qiong , Wang Di , Wang Lin 
Published: March 2024

IntroductionContinuous strawberry cropping often causes soil-borne diseases, with 20 calcium cyanamide being an effective soil fumigant, pig manure can often be used as soil organic fertilizer. Its impact on soil microorganisms structure, however, remains unclear.MethodsThis study investigated the effectiveness of calcium cyanamide and pig manure in treating strawberry soil, specifically against strawberry anthracnose. We examined the physical and chemical properties of the soil and the rhizosphere microbiome and performed a network analysis.ResultsResults showed that calcium cyanamide treatment significantly reduces the mortality rate of strawberry in seedling stage by reducing pathogen abundance, while increasing actinomycetes and Alphaproteobacteria during the harvest period. This treatment also enhanced bacterial network connectivity, measured by the average connectivity of each Operational Taxonomic Unit (OTU), surpassing other treatments. Moreover, calcium cyanamide notably raised the levels of organic matter, available potassium, and phosphorus in the soil–key factors for strawberry disease resistance and yield.DiscussionOverall, applying calcium cyanamide to soil used for continuous strawberry cultivation can effectively decrease anthracnose incidence. It may be by changing soil physical and chemical properties and enhancing bacterial network stability, thereby reducing the copy of anthracnose. This study highlights the dual benefit of calcium cyanamide in both disease control and soil nutrient enhancement, suggesting its potential as a valuable tool in sustainable strawberry farming.