bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-generated mechanical forces play a role in epileptogenesis after injury
Authors: Dalir Laya , Tatic-Lucic Svetlana , Berdichevsky Yevgeny 
Published: February 2025

AbstractTraumatic brain injury (TBI) is associated with a significantly increased risk of epilepsy. One of the consequences of severe TBI is progressive brain atrophy, which is frequently characterized by organized tissue retraction. Retraction is an active process synchronized by mechanical interactions between surviving cells. This results in unbalanced mechanical forces acting on surviving neurons, potentially activating mechanotransduction and leading to hyperexcitability. This novel mechanism of epileptogenesis was examined in organotypic hippocampal cultures, which develop spontaneous seizure-like activity in vitro. Cell-generated forces in this model resulted in contraction of hippocampal tissue. Artificial imbalances in mechanical forces were introduced by placing cultured slices on surfaces with adhesive and non-adhesive regions. This modeled disbalance in mechanical forces that may occur in the brain after trauma. Portions of the slices that were not stabilized by substrate adhesion underwent increased contraction and compaction, revealing the presence of cell-generated forces capable of shaping tissue geometry. Changes in tissue geometry were followed by excitability changes that were specific to hippocampal sub-region and orientation of contractile forces relative to pyramidal cell apical-basal axis. Results of this study suggest that imbalanced cell-generated forces contribute to development of epilepsy, and that force imbalance may represent a novel mechanism of epileptogenesis after trauma.
Assessment of native mass spectrometry as a screening method to identify and characterize RNA-targeting small molecules
Authors: Sternicki Louise M. , Klose Jack W. , Poulsen Sally-Ann 
Published: February 2025

ABSTRACTThe intentional targeting of RNA with small molecules is recognized as a viable pathway to new therapeutics with potential to vastly expand the proportion of the human genome considered druggable. The practical considerations to deliberately target RNA are largely under development, including optimal screening methods to identify small molecules for binding to RNA. Native mass spectrometry (nMS) is established as a valuable biophysical method for identifying small molecule hits against diverse biomolecular targets, most frequently proteins and protein-protein interactions. Herein we assess nMS for studying the binding of small molecules with RNA aptamers as a model for nMS screening of RNA as a drug target. We first develop workflows for characterizing the binding of cognate RNA aptamer ligands and then establish a nMS method to screen a small molecule library against the RNA aptamers. nMS analysis permitted identification of binders, quantitation of binding strength and generation of structure-activity relationships with some dependence on the aptamer class. This work demonstrates the utility of nMS as a complementary and efficient target-based biophysical screening method that can characterize RNA-small molecule interactions and the potential of nMS becoming a powerful enabling tool in RNA-targeting drug discovery.
CandyCollect Open to Closed (O2C) Microfluidic System for Rapid and User-Centric Detection of Group A Streptococcus
Authors: Leong Kelsey M. , Sanchez J. Carlos , Craig Cosette , Tatka John A. , Arvizu René R. , Robertson Ingrid H. , Shinkawa Victoria A. M. , Robinson Timothy R. , Chang Megan M. , Su Xiaojing , Thongpang Sanitta , Theberge Ashleigh B. , Berthier Erwin , Olanrewaju Ayokunle O. 
Published: February 2025

AbstractWe created CandyCollect, a lollipop-inspired, child-friendly saliva sampling device that provides enhanced comfort compared to common throat and mouth swabs. The device has an open microchannel and a functionalized surface that effectively captures and stores the saliva and pathogens. We recently demonstrated the ability to combine the CandyCollect with rapid antigen detection tests (RADTs) for fast and accessible detection of group A streptococcus (GAS).However, the current procedure necessitates manual steps to elute and transfer captured antigens from the lollipop to RADTs, which relies on the user to follow complex instructions, increasing the likelihood of error. In this work, we developed the CandyCollect Open to Closed (O2C) microfluidic system to automate antigen elution and streamline detection with RADTs. The O2C system securely seals the open microchannels of the CandyCollect device to create a continuous closed channel for seamless delivery of elution reagents to release antigens at the press of a button for subsequent lateral flow detection. Our system leverages and combines the unique benefits of both open channel and closed channel microfluidics into one platform, further enhancing the potential of both methods. We performed experiments with GAS bacteria spiked into saliva and compared the manual and O2C elution methods. Both the manual and O2C methods showed clear test lines on the RADT strips at clinically relevant GAS concentrations (between 5 ×105and 109CFU/mL). The O2C provided comparable or better results than the manual procedure in a more convenient form factor. The O2C platform has the potential to enable user-friendly screening for respiratory pathogens by minimally trained users in decentralized settings.
Defects in nephrogenesis result in an expansion of theFoxd1+ stromal progenitor population
Authors: Michalopulos Michael G. , Liu Yan , Raju Dinesh Ravindra , Lafin John T. , Ma Yanru , Gaur Dhruv , Khadka Sadiksha , Xing Chao , McMahon Andrew P. , Carroll Thomas J. , Drake Keri A. 
Published: February 2025

ABSTRACTReciprocal signaling interactions coordinate multiple aspects of kidney development. While signals from the stroma have been shown to regulate nephron progenitor cell (NPC) differentiation, much less is known about regulation of the stromal progenitor population. Here, we demonstrate that disruption of the NPC lineage via loss ofWt1(i.e.,Six2cre;Wt1c/c) results in an expansion ofFoxd1+ stromal progenitor cells. Analyses of the developing stroma in two additional models, includingWnt4-nullmutants (which fail to form nephron structures similar toSix2cre;Wt1c/ckidneys) and NPC ablation via diphtheria toxin (i.e.,Six2cre;RosaDTAc/+), both phenocopySix2cre;Wt1c/cmutants, thus further confirming that defects in the NPC lineage result in abnormal development of the stromal progenitor population. Furthermore, we identify a subcluster of theFoxd1+ stroma that appears expanded in the three mutant mouse models and conserved in human fetal kidneys. Overall, the findings from this study suggest that loss of differentiating nephron structures may result in possible over proliferation of the stromal progenitor population and/or a block in stromal differentiation and further highlight how crosstalk amongst the progenitor cell lineages coordinates multiple aspects of kidney development.KEY POINTSMutant mouse models targeting the nephron lineage (i.e.,Six2cre;Wt1c/candWnt4-nullmutants which fail to form early nephron structures) suggest that a block in NPC differentiation results in an abnormal expansion of theFoxd1+ stromal progenitor population.Mutant kidneys with NPC ablation (i.e.,Six2cre;RosaDTAc/+) show maintenance of stromal progenitors independent of signals from adjacent nephron progenitors and ureteric bud.Single nuclei RNA-seq identifies three subclusters of theFoxd1+ stromal progenitor population at E15.5, including one cluster of proliferating cells and a distinctFap+,Igf1+,Gria1+,Gdnf– subcluster which appears expanded inSix2cre;Wt1c/c,Wnt4-null,andSix2cre;RosaDTAc/+mutant kidneys and conserved in human fetal kidneys.
The association between virtual communication and leadership in the post-pandemic era: the role of emotional intelligence
Authors: Chouhan Vikram Singh , Shukla Abhishek 
Published: February 2025

PurposeThe study aims to examine the association between virtual communication effectiveness (VCE), leadership effectiveness (LE) and the role of emotional intelligence (EI) in the post-pandemic era.Design/methodology/approachA survey was conducted among 305 employees in the Indian IT sector using an online questionnaire. Data were analyzed using reliability, validity and moderated regression analysis.FindingsThe findings reveal that VCE is a significant predictor of LE. EI plays a significant moderating role between VCE and LE.Originality/valueThis study establishes the role of EI in pre-empting LE. Furthermore, it results in the advancement of improved tools for the selection, training and development of leadership talent. Research on virtual communication (VC) and EI enhances our understanding of effective leadership. To the best of the authors’ knowledge, the present research is one of the first to link and standardize various practices of VC, and EI to increase LE in the post-pandemic era.
Data-driven identification method for bolt looseness of complicated tower structure
Authors: Gao Yingbo , Yan Bo , Yang Hanxu , Deng Mao , Lv Zhongbin , Zhang Bo , Liu Guanghui 
Published: February 2025

PurposeA transmission tower usually experiences bolt loosening under long-term alternating cyclic load, which may lead to collapse of the tower in extreme operating conditions. The paper aims to propose a data-driven identification method for bolt looseness of complicated tower structures based on reduced-order models and numerical simulations to perceive and evaluate the health state of a tower in operation.Design/methodology/approachThe equivalent stiffnesses of three types of bolt joints under various loosening scenarios are numerically determined by three-dimensional finite element (FE) simulations. The order of the FE model of a tower structure with bolt loosening is reduced by means of the component modal synthesis method, and the dynamic responses of the reducer-order model under calibration loads are simulated and used to create the dataset. An identification model for bolt looseness of the tower structure based on convolutional neural networks driven by the acceleration sensors is constructed.FindingsAn identification model for bolt looseness of the tower structure based on convolutional neural networks driven by the acceleration sensors is constructed and the applicability of the model is investigated. It is shown that the proposed method has a high identification accuracy and strong robustness to data noise and data missing. Meanwhile, the method is less dependent on the number and location of sensors and is easier to apply in real transmission lines.Originality/valueThis paper proposes a data-driven identification method for bolt looseness of a complicated tower structure based on reduced-order models and numerical simulations. Non-linear relationships between equivalent stiffness of bolted joints and bolt preload depicting looseness are obtained and reduced-order model of tower structure with bolt looseness is established. Finally, this paper investigates applicability of identification model for bolt looseness.
Framework for detecting, assessing and mitigating mental health issue in the context of online social networks: a viewpoint paper
Authors: Roggendorf Polina , Volkov Andrei 
Published: February 2025

PurposeThe development and presentation of a framework that integrates modern methods for detecting, assessing and mitigating mental health issues in the context of dynamic and adverse changes in social networks.Design/methodology/approachThis viewpoint is based on a literature review of current advancements in the field. The use of causal discovery and causal inference methods forms the foundation for applying all the techniques included in the framework (machine learning, deep learning, explainable AI as well as large language models and generative AI). Additionally, an analysis of network effects and their influence on users’ emotional states is conducted.FindingsThe synergy of all methods used in the framework, combined with causal analysis, opens new horizons in predicting and diagnosing mental health disorders. The proposed framework demonstrates its applicability in providing additional analytics for the studied subjects (individual traits and factors that worsen mental health). It also proves its ability to identify hidden factors and processes.Originality/valueThe proposed framework offers a novel perspective on addressing mental health issues in the context of rapidly evolving digital platforms. Its flexibility allows for the adaptation of tools and methods to various scenarios and user groups. Its application can contribute to the development of more accurate algorithms that account for the impact of negative (including hidden) external factors affecting users. Furthermore, it can assist in the diagnostic process.
Surviving a cognitive crisis at work: understanding the causes and consequences of cognitive dissonance
Authors: Shah Javaid Ali , Lacaze Delphine 
Published: February 2025

Purpose
The purpose of this paper is twofold. First, most of the previous studies have primarily studied the cognitive dissonance outcomes of a single organization’s employees and have used a quantitative approach to study cognitive dissonance. This study attempts to improve this shortcoming and explores the cognitive dissonance in employees of different workplaces. Furthermore, multiple previous related studies focused on a specific stage rather than the whole process of cognitive dissonance. Current study tends to explain the process by systematically postulating the antecedents and conflicts faced by employees that cause cognitive dissonance and effect their personal and professional lives.

Design/methodology/approach
Semi-structured interviews were used to collect data from two samples, i.e. France and Pakistan. A total of 30 interviews were performed to collect data about employees’ work environments and dissonant situations. Gioia’s methodology of two-order thematic analyses was used, and multiple themes were produced that were embedded in the context of cognitive dissonance.

Findings
The qualitative study revealed that the employees exposed to cognitive dissonance suffered in both professional and individual lives in different manners (physically and mentally).

Practical implications
This study contributes significantly to cognitive dissonance theory by highlighting the powerful urge for dissonance reduction during dissonant experiences. It also emphasizes the detrimental influence of work contextual factors, such as unfairness and injustice on employee positivity. This study suggests that researching dissonance reduction strategies can be a valuable resource for effective organizational interventions. By promoting a workplace where employees, stakeholders and organizational agents can cooperate and communicate effectively in decision-making processes.

Originality/value
In the current paper, the authors considered improving the cognitive dissonance theory, primarily conceptual inadequacies and its connection with work ethics. As a better comprehension of the methodological perspectives is imperative to future hypothetical advancement, the authors proposed some approaches to address these deficiencies.
Competitive and polyphonic reactivity to accountability pressures in digital public sector reporting: insights from Italian state museums
Authors: Agostino Deborah 
Published: February 2025

PurposeThis study investigates the reactions of public sector organisations to the accountability pressures of online dashboards and the impact of this reactivity on reporting systems. This study explores the concept of “reactivity,” which explains changes in organisational behaviours in reaction to being evaluated, observed or measured.Design/methodology/approachThis study adopts a case study methodology to investigate organisations’ reactivity to an online dashboard within the Italian system of state museums. Data were gathered through a real-time dashboard initiated in 2019, complemented by interviews, participant observations and secondary sources collected over a period of three years.FindingsThe results indicate that online dashboards provoke a divergent but coexistent response: competitive reactivity when museums modify their behaviours to improve performance disclosed in the online dashboard, and polyphonic reactivity when museums question the algorithm’s validity and accuracy on the basis of the online dashboard. This finding reveals the emergence of layered accountability, with organisations balancing public expectations and hierarchical standards through manipulation and boycott, thereby giving rise to a decoupled system of measures.Originality/valueThis research contributes to the literature on public sector accountability by highlighting the dual nature of reactivity, both conforming to and resisting the imposed metrics. This study enhances our understanding of the interplay between digital accountability mechanisms and organisational responses, offering insights into the complexities of adapting to real-time, data-driven environments in the public sector.
Prioritisation of barriers in the journey of regenerative tourism – a DEMATEL perspective
Authors: Agarwal Alpana , Ojha Ravindra 
Published: February 2025

PurposeHumans are the most significant entity in the fast-growing tourism ecosystem. Paradoxically, many of the environmental crises caused by tourism are the consequences of anthropocentrism. Therefore, initiatives for regenerative tourism are imperative. This paper aims to identify and understand the key barriers and their interrelationships under regenerative tourism and provide recommendations for progress.Design/methodology/approachThe literature review and focus group approach identified barriers to regenerative tourism. Then, the Decision-Making Trial and Evaluation Laboratory (DEMATEL) methodology was applied to analyse the strength and cause-and-effect interrelationships between the barriers.FindingsThe scatter plot and digraph of the data set from DEMATEL have provided useful insights. The strongest barrier is the absence of an evolved comprehensive framework for regenerative tourism for each location. A linear mindset and lack of a single-window approach for regenerative initiatives are the two cause barriers. The two strongest effect barriers are the lack of symbiotic relationship/management between various entities in the tourism ecosystem, and operational barriers focused only on short-term routine business objectives.Practical implicationsThe DEMATEL approach has brought out useful stakeholder insights. It has set the priorities for focus based on the degree of interrelationship strength and the relationship’s kind (cause or effect). It has provided stakeholders for futuristic regenerative tourism.Originality/valueApplication of the DEMATEL approach for analysing the cause-and-effect dynamics between the twelve barriers to the future of regenerative tourism.
Caring for innovation: practices of compassion in health and social care
Authors: Manfrini Caterina , Duff Cameron 
Published: February 2025

PurposeAcross the public sector, and especially in the delivery of health and social care and support, practicing innovation is a difficult and seldom rewarding activity. The organisational barriers inhibiting innovation adoption in healthcare settings have been widely discussed. What is less well understood is what motivates staff to persist with innovation efforts despite these barriers. This paper contributes to recent studies of the role of care and compassion in innovation processes within social care settings not only to generate new insights into the motivations underpinning innovation efforts but also to help illuminate how staff overcome barriers to innovation.Design/methodology/approachWe crafted a series of vignettes from our recent ethnographic studies of innovation across the health and social care sectors in Australia and Denmark, involving semi-structured interviews, observation and field notes. Within the Danish case, we explore an instance involving a formal organisational focus on identifying and sustaining innovation within local service deliver. With the Australian case, we present an informal approach, where the process of identifying and sustaining innovation derives from moments of spontaneous employee engagement and initiative.FindingsReflecting on the examples of “frugal innovation” presented in these vignettes, the major contribution of this study is to situate care and compassion as critical social, affective and material aspects of the practice of innovation in health and social care settings. Our analysis indicates how the practice of innovation is shaped by diverse relations of caregiving, where compassion emerges as a key source of motivation, aspiration and application that inspires staff to seek novel solutions to enduring healthcare challenges.Originality/valueWe develop our argument with reference to recent interdisciplinary orientations to care and compassion in the healthcare literature, incorporating contributions from feminist scholars and the ongoing articulation of feminist care ethics in the study of innovation.
Lived experiences and the structure of shared leadership in entrepreneurial firms: delegation, empowerment and performance
Authors: Chandranathan Preman 
Published: February 2025

PurposeThis paper aims to discuss findings from a study which explored the lived experiences of leadership among owner-managers and employees of growing entrepreneurial firms. In doing so it contributes to the conceptual development of entrepreneurial leadership via shared leadership theory and qualitative empirical material.Design/methodology/approachThe study utilised a qualitative, interpretivist and comparative case study approach. Findings were developed from the analysis of 38 interviews conducted with organisational members of 5 entrepreneurial firms located in the northwest of England.FindingsThe findings illustrate an understanding of shared entrepreneurial leadership, which is premised upon organisational ownership and characterized by three mutually interacting forms of influence in the settings of interest. These three forms of influence are: (1) hierarchical downward influence, which includes (2) empowerment as a means of enabling employees so they are positioned to influence work processes and (3) lateral peer influence within teams.Research limitations/implicationsThis paper also highlights the importance of organisational ownership as a contextual factor that may influence entrepreneurial leadership. It offers recommendations for further research in this regard to develop entrepreneurial leadership research.Practical implicationsFor practitioners, this paper highlights the dynamics of a more collectivist approach to leadership and how this might be practiced within entrepreneurial firms. The findings illustrate more trust, delegation and empowerment on the part of hierarchical leaders and mutuality and co-performance on the part of organisational members as all involved work collaboratively towards objectives associated with venture-building.Originality/valueThis paper contributes a contextualised, nuanced account of shared leadership processes and dynamics in owner-managed, growth-oriented entrepreneurial firms, representing the first to do so via qualitative insights in entrepreneurial leadership research.