bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome‐wide identification of XTH gene family in Musa acuminata and response analyses of MaXTHs and xyloglucan to low temperature
Authors: Tan Yehuan , Zhan Huiling , Chen Houbin , Li Xiaoquan , Chen Chengjie , Liu Hui , Chen Yilin , Zhao Ziyue , Xiao Yinyan , Liu Jing , Zhao Yafang , Su Zuxiang , Xu Chunxiang 
Published: February 2024

AbstractBanana (Musa spp.) production is seriously threatened by low temperature (LT) in tropical and subtropical regions. Xyloglucan endotransglycosylase/hydrolases (XTHs) are considered chief enzymes in cell wall remodelling and play a central role in stress responses. However, whether MaXTHs are involved in the low temperature stress tolerance in banana is not clear. Here, the identification and characterization of MaXTHs were carried out, followed by prediction of their cis‐acting elements and protein–protein interactions. In addition, candidate MaXTHs involved in banana tolerance to LT were screened through a comparison of their responses to LT between tolerant and sensitive cultivars using RNA‐Seq analysis. Moreover, immunofluorescence (IF) labelling was employed to compare changes in the temporal and spatial distribution of different types of xyloglucan components between these two cultivars upon stress.In total, 53 MaXTHs have been identified, and all were predicted to be located in the cell wall, 14 of them also in the cytoplasm. Only 11 MaXTHs have been found to interact with other proteins. Among 16 MaXTHs with LT responsiveness elements, MaXTH26/29/32/35/50 (Group I/II members) and MaXTH7/8 (Group IIIB members) might be involved in banana tolerance to LT stress. IF results suggested that the content of xyloglucan components recognized by CCRC‐M87/103/104/106 antibodies might be negatively related to banana chilling tolerance. In conclusion, we have identified the MaXTH gene family and assessed cell wall re‐modelling under LT stress. These results will be beneficial for banana breeding against stresses and enrich the cell wall‐mediated resistance mechanism in plants to stresses.
Predictors of non‐fatal suicide attempts among Veterans Health Administration (VHA) patients who experienced military sexual trauma
Authors: Hein Tyler C. , Austin Karen , Grau Peter P. , Keith Jessica A. , Claes Nathan J. , Bowersox Nicholas W. 
Published: February 2024

AbstractObjectiveMilitary sexual trauma (MST) has been identified as a risk factor for suicidal behavior. To inform suicide prevention efforts within the Veterans Health Administration (VHA), this study evaluates predictors of non‐fatal suicide attempts (NFSAs) among VHA patients who experienced MST.MethodsFor VHA patients in fiscal year (FY) 2019 who previously screened positive for a history of MST, documented NFSAs were assessed. Using multivariable logistic regression, demographic, clinical, and VHA care utilization predictors of NFSAs were assessed.ResultsOf the 212,215 VHA patients who screened positive for MST prior to FY 2019 and for whom complete race, service connection, and rurality information was available, 1742 (0.8%) had a documented NFSA in FY 2019. In multivariable logistic regression analyses, total physical and mental health morbidities were not associated with NFSA risk. Predictors of a documented NFSA included specific mental health diagnoses [adjusted odds ratio (aOR) range: 1.28–1.94], receipt of psychotropic medication prescriptions (aOR range: 1.23–2.69) and having a prior year emergency department visit (aOR = 1.32) or inpatient psychiatric admission (aOR = 2.15).ConclusionsAmong VHA patients who experienced MST, specific mental health conditions may increase risk of NFSAs, even after adjustment for overall mental health morbidity. Additionally, indicators of severity of mental health difficulties such as receipt of psychotropic medication prescriptions and inpatient psychiatric admissions are also associated with increased risk above and beyond risk associated with diagnoses. Findings highlight targets for suicide prevention initiatives among this vulnerable group within VHA and may help identify patients who would benefit from additional support.
Skinly: A novel handheld IoT device for validating biophysical skin characteristics
Authors: Tobar Maria del Pilar Bonilla , Clemann Sven , Hagens Ralf , Pagel‐Wolff Sonja , Hoppe Stefan , Behm Peter , Engelhard Felicia , Langhals Maria , Gallinat Stefan , Zhavoronkov Alex , Georgievskaya Anastasia , Kiselev Konstantin , Tlyachev Timur , Jaspers Sören 
Published: February 2024

AbstractBackgroundRecent advancements in artificial intelligence have revolutionized dermatological diagnostics. These technologies, particularly machine learning (ML), including deep learning (DL), have shown accuracy equivalent or even superior to human experts in diagnosing skin conditions like melanoma. With the integration of ML, including DL, the development of at home skin analysis devices has become feasible. To this end, we introduced the Skinly system, a handheld device capable of evaluating various personal skin characteristics noninvasively.Materials and MethodsEquipped with a moisture sensor and a multi‐light‐source camera, Skinly can assess age‐related skin parameters and specific skin properties. Utilizing state‐of‐the‐art DL, Skinly processed vast amounts of images efficiently. The Skinly system's efficacy was validated both in the lab and at home, comparing its results to established “gold standard” methods.ResultsOur findings revealed that the Skinly device can accurately measure age‐associated parameters, that is, facial age, skin evenness, and wrinkles. Furthermore, Skinly produced data consistent with established devices for parameters like glossiness, skin tone, redness, and porphyrin levels. A separate study was conducted to evaluate the effects of two moisturizing formulations on skin hydration in laboratory studies with standard instrumentation and at home with Skinly.ConclusionThanks to its capability for multi‐parameter measurements, the Skinly device, combined with its smartphone application, holds the potential to replace more expensive, time‐consuming diagnostic tools. Collectively, the Skinly device opens new avenues in dermatological research, offering a reliable, versatile tool for comprehensive skin analysis.
Estimated median density identifies donor age and sex differences in red blood cell biological age
Authors: Mykhailova Olga , Brandon‐Coatham Mackenzie , Durand Kiarra , Olafson Carly , Xu April , Yi Qi‐Long , Kanias Tamir , Acker Jason P. 
Published: February 2024

AbstractBackgroundDonors possess heterogeneous red cell concentrates (RCCs) in terms of the biological age of their red blood cells (RBCs) as a direct result of various donor‐dependent factors influencing rates of erythropoiesis. This study aimed to estimate the median biological age of RBCs in RCCs based on donor age and sex to investigate inherent differences in blood products' biological ages over hypothermic storage using estimated median densities (EMDs).Study DesignSixty RCCs were collected from four donor groups; male and female teenagers (17–19 years old) and seniors (75+ years old). A Percoll density‐based separation approach was used to quantify the EMDs indicative of biological age. EMD and mean corpuscular hemoglobin (MCHC) were compared by correlation analyses.ResultsDifferences in the median biological age of RCC units were observed with male donors having significantly higher EMDs compared to females (p < .001). Teen male donors possessed the highest EMDs with significantly elevated levels of biologically aged RBCs compared to both female donor groups, regardless of storage duration (p < .05). Throughout most of the 42‐day storage period, senior donors, particularly senior females, demonstrated the strongest correlation between EMD and MCHC (R2 > 0.5).ConclusionsThis study provides further evidence that there are inherent differences between the biological age profiles of RBCs between blood donors of different sex and age. Our findings further highlight that biological age may contribute to RBC quality during storage and that donor characteristics need to be considered when evaluating transfusion safety and efficacy.
Significant changes to AABB Standards: Updated quality system essentials
Authors: Ooley Patrick , Bocquet Christopher 
Published: February 2024

AbstractIn the late 1990s, AABB joined many other accrediting organizations in embracing a quality system approach to setting standards. This change, seen at the time as the new era of standards‐setting, was marked by a shift from error detection to error prevention as the main emphasis. AABB termed the framework for this approach “quality system essentials (QSEs).” These QSEs were incorporated into all eight AABB Standards. The QSEs enhanced the existing technical requirements and have been updated regularly since then for all operational areas in which AABB sets standards and provides accreditation.
Gastrointestinal colonization by Diutina (Candida) rugosa in a 6‐year‐old Siberian Husky
Authors: Randolph Nina K. , Her Jiwoong , McAloney Camille A. , Wellman Maxey 
Published: February 2024

AbstractA 6‐year‐old 21.5 kg castrated male Siberian Husky was presented for acute onset of lethargy, vomiting, hemorrhagic diarrhea, and inappetence. Physical examination revealed marked discomfort upon abdominal palpation and 5%–7% dehydration. The CBC and biochemical profile revealed changes consistent with mild to moderate inflammation, dehydration, and gastrointestinal (GI) disease. Despite aggressive gastrointestinal support, anorexia persisted, and an upper GI endoscopy was performed in conjunction with esophagostomy tube placement. Endoscopy revealed abnormal gastric mucosa characterized by moderately well‐demarcated areas of blue‐black discoloration. Impression smears of a gastric biopsy revealed abundant extracellular yeasts with morphology most consistent with Candida spp. and frequent extracellular cocci. Similar yeast and bacteria, in lower numbers, were observed on cytologic analysis of a direct smear of the rectal mucosa. A rectal swab submitted for fungal culture yielded pure growth of fungal yeasts identified as Diutina (formerly Candida) rugosa by matrix‐assisted laser desorption‐ionization time‐of‐flight mass spectrometry. The dog's clinical signs improved with fluconazole, and he was discharged. Follow‐up fungal culture of a rectal swab showed no growth of D. rugosa. To the authors' knowledge, this is the first case report that describes the clinical, hematologic, cytologic, and gross findings of enteric colonization by D. rugosa in a dog.
Modeling of Vehicle Mobility in Shallow Water With Data-Driven Hydrodynamics Model
Authors: Yamashita Hiroki , Martin Juan Ezequiel , Tison Nathan , Grunin Arkady , Jayakumar Paramsothy , Sugiyama Hiroyuki 
Published: February 2024

Abstract
In this study, a data-driven hydrodynamics model is proposed to enable quick prediction of vehicle mobility in shallow water, considering the effect of tire–soil interaction. To this end, a high-fidelity coupled vehicle–water interaction model using computational fluid dynamics (CFD) and multibody dynamics (MBD) solvers is developed to characterize the hydrodynamic loads exerted on a vehicle operated in shallow water, and it is used to generate training data for the data-driven hydrodynamics model. To account for the history-dependent hydrodynamic behavior, a long short-term memory (LSTM) neural network is introduced to incorporate effects of the historical variation of vehicle motion states as the input to the data-driven model, and it is used to predict hydrodynamic loads online exerted on vehicle components in the MBD mobility simulation. The impacts of hydrodynamic loads on the vehicle mobility capability in shallow water are examined for different water depths and incoming flow speeds using the high-fidelity coupled CFD-MBD model. Furthermore, it is demonstrated that the vehicle–water interaction behavior in scenarios not considered in the training data can be predicted using the proposed LSTM data-driven hydrodynamics model. However, the use of non-LSTM layers, which do not account for the sequential variation of vehicle motion states as the input, leads to an inaccurate prediction. A substantial computational speedup is achieved with the proposed LSTM-MBD vehicle–water interaction model while ensuring accuracy, compared to the computationally expensive high-fidelity coupled CFD-MBD model.
Cutaneous
Leishmania mexicana
infections in the United States: defining strains through endemic human pediatric cases in northern Texas
Authors: Nepal Binita , McCormick-Baw Clare , Patel Karisma , Firmani Sarah , Wetzel Dawn M. 
Published: February 2024

ABSTRACT

Over a 6-month span, three patients under 5 years old with cutaneous leishmaniasis presented to the Pediatric Infectious Diseases Clinic at the University of Texas Southwestern Medical Center/Children’s Health Dallas. None had traveled outside of northern Texas/southern Oklahoma; all had
Leishmania mexicana
infections confirmed by PCR. We provide case descriptions and images to increase the awareness of this disease among United States (US) physicians and scientists. Two patients responded to fluconazole, but the youngest required topical paromomycin. Combining these cases with guidelines and our literature review, we suggest that (i) higher doses (10–12 mg/kg/day) of fluconazole should be considered in young children to maximize likelihood and rapidity of response and (ii) patients should transition to alternate agents if they do not respond to high-dose fluconazole within 6 weeks. Furthermore, and of particular interest to the broad microbiology community, we used samples from these cases as a proof of concept to propose a mechanism to strain-type US-endemic
L. mexicana
. For our analysis, we sequenced three housekeeping genes and the internal transcribed sequence 2 of the ribosomal RNA gene. We identified genetic changes that not only allow us to distinguish US-based
L. mexicana
strains from strains found in other areas of the Americas but also establish polymorphisms that differ between US isolates. These techniques will allow documentation of genetic changes in this parasite as its range expands. Hence, our cases of cutaneous leishmaniasis provide significant evolutionary, treatment, and public health implications as climate change increases exposure to formerly tropical diseases in previously non-endemic areas.

IMPORTANCE

Leishmaniasis is a parasitic disease that typically affects tropical regions worldwide. However, the vector that carries
Leishmania
is spreading northward into the United States (US). Within a 6-month period, three young cutaneous leishmaniasis patients were seen at the Pediatric Infectious Diseases Clinic at the University of Texas Southwestern Medical Center/Children's Health Dallas. None had traveled outside of northern Texas and southern Oklahoma. We document their presentations, treatments, and outcomes and compare their management to clinical practice guidelines for leishmaniasis. We also analyzed the sequences of three critical genes in
Leishmania mexicana
isolated from these patients. We found changes that not only distinguish US-based strains from strains found elsewhere but also differ between US isolates. Monitoring these sequences will allow tracking of genetic changes in parasites over time. Our findings have significant US public health implications as people are increasingly likely to be exposed to what were once tropical diseases.
Identification of intratumor bacteria-associated prognostic risk score in adrenocortical carcinoma
Authors: Tan Linyi , Zhang Dengwei , Li Yong-xin , Li Yuqing , Guo Ting , Sun Yang , Li Ning , Feng Chenchen 
Published: February 2024

ABSTRACT

A landmark study by Poore et al. showed intratumor bacteria (ITBs) playing a critical role in most cancers by reproduction of The Cancer Genome Atlas (TCGA) transcriptome data. A recent study by Salzberg et al. argued that ITBs, being overstated as a methodology by Poore et al., were problematic. We previously reported that ITBs were prognostic in adrenocortical carcinoma (ACC), a highly aggressive rare disease using data by Poore et al., and here, we aimed to answer whether ITBs truly existed and were prognostic in ACC. ACC samples from our institutes underwent 16S rRNA sequencing [adrenocortical carcinoma blocks from Huashan Hospital and China Medical University (HS) cohort]. The ITB profile was compared to TCGA data processed by Poore et al. (TCGA-P) and TCGA data processed by Salzberg et al. (TCGA-S), respectively. The primary outcome was overall survival (OS). A total of 26 ACC cases (HS cohort) and 10 paraffin controls were sequenced. The TCGA cohort encompassed 77 cases. Two and four amid the top 10 abundant genera in HS cohort were not detected in TCGA-P and TCGA-S, respectively. Neither was alpha or beta diversity associated with survival nor could ACC be subtyped by ITB signature in the HS cohort. Notably, a five-genera ITB risk score (
Corynebacterium
,
Mycoplasma
,
Achromobacter
,
Anaerococcus
, and
Streptococcus
) for OS trained in the HS cohort was validated in both TCGA-P and TCGA-S cohorts and was independently prognostic. Whereas ITB signature on the whole may not be associated with ACC subtypes, certain ITB features are associated with prognosis, and a risk score could be generated and validated externally.

IMPORTANCE
In this report, we looked at the role of ITBs in ACC in patients with different race and sequencing platforms. We found a five-genera ITB risk score consistently predicted overall survival in all cohorts. We conclude that certain ITB features are universally pathogenic to ACC.
Impact of topical applications of sunflower seed oil on neonatal mortality and morbidity in southern Nepal: a community-based, cluster-randomised trial
Authors: Katz Joanne , Khatry Subarna K , Shrestha Laxman , Summers Aimee , Visscher Marty O , Sherchand Jeevan B , Tielsch James M , Subedi Seema , LeClerq Steven C , Mullany Luke C 
Published: February 2024

IntroductionHospital-based studies have demonstrated topical applications of sunflower seed oil (SSO) to skin of preterm infants can reduce nosocomial infections and improve survival. In South Asia, replacing traditional mustard with SSO might have similar benefits.Methods340 communities in Sarlahi, Nepal were randomised to use mustard oil (MO) or SSO for community practice of daily newborn massage. Women were provided oil in late pregnancy and the first month post partum, and visited daily through the first week of life to encourage massage practice. A separate data collection team visited on days 1, 3, 7, 10, 14, 21 and 28 to record vital status and assess serious bacterial infection.ResultsBetween November 2010 and January 2017, we enrolled 39 479 pregnancies. 32 114 live births were analysed. Neonatal mortality rates (NMRs) were 31.8/1000 (520 deaths, 16 327 births) and 30.5/1000 (478 deaths, 15 676 births) in control and intervention, respectively (relative risk (RR)=0.95, 95% CI: 0.84, 1.08). Among preterm births, NMR was 90.4/1000 (229 deaths, 2533 births) and 79.2/1000 (188 deaths, 2373 births) in control and intervention, respectively (RR=0.88; 95% CI: 0.74, 1.05). Among preterm births <34 weeks, the RR was 0.83 (95% CI: 0.67, 1.02). No statistically significant differences were observed in incidence of serious bacterial infection.ConclusionsWe did not find any neonatal mortality or morbidity benefit of using SSO instead of MO as emollient therapy in the early neonatal period. Further studies examining whether very preterm babies may benefit are warranted.Trial registration numberClinicalTrials.gov Registry (NCT01177111).
Dark energy and dark matter configurations for wormholes and solitionic hierarchies of nonmetric Ricci flows and $$F(R,T,Q,T_{m})$$ gravity
Authors: Bubuianu Laurenţiu , Vacaru Sergiu I. , Veliev Elşen Veli , Zhamysheva Assel 
Published: February 2024

AbstractWe extend the anholonomic frame and connection deformation method, AFCDM, for constructing exact and parametric solutions in general relativity, GR, to geometric flow models and modified gravity theories, MGTs, with nontrivial torsion and nonmetricity fields. Following abstract geometric or variational methods, we can derive corresponding systems of nonmetric gravitational and matter field equations which consist of very sophisticate systems of coupled nonlinear PDEs. Using nonholonomic frames with dyadic spacetime splitting and applying the AFCDM, we prove that such systems of PDEs can be decoupled and integrated in general forms for generic off-diagonal metric structures and generalized affine connections. We generate new classes of quasi-stationary solutions (which do not depend on time like coordinates) and study the physical properties of some physically important examples. Such exact or parametric solutions are determined by nonmetric solitonic distributions and/or ellipsoidal deformations of wormhole hole configurations. It is not possible to describe the thermodynamic properties of such solutions in the framework of the Bekenstein–Hawking paradigm because such metrics do not involve, in general, certain horizons, duality, or holographic configurations. Nevertheless, we can always elaborate on associated Grigori Perelman thermodynamic models elaborated for nonmetric geometric flows. In explicit form, applying the AFCDM, we construct and study the physical implications of new classes of traversable wormhole solutions describing solitonic deformation and dissipation of non-Riemannian geometric objects. Such models with nontrivial gravitational off-diagonal vacuum are important for elaborating models of dark energy and dark matter involving wormhole configurations and solitonic-type structure formation.