bluuebunny/crossref_metadata_2025_split huggingface dataset:
In-depth investigation of the mechanisms of high and low residual feed intake regulating hens during the late laying period via liver and gut microbiota
Authors: Gao Zhouyang , Zheng Chuanwei , Mao Zhiqiong , Zheng Jiangxia , Liu Dan , Xu Guiyun 
Published: March 2024

AbstractResidual feed intake (RFI) is a more accurate indicator of feed efficiency than the feed conversion ratio (FCR) and is widely used to measure the efficiency of livestock and poultry feed utilization. Typically, Low RFI (LRFI) implies higher feed conversion efficiency, while high RFI (HRFI) indicates lower feed conversion efficiency. This study systematically explored the differences between high and low RFI and the function of the liver and cecum microbes of hens during the late laying period by multiple-omics techniques and further explored the interaction among microorganisms, the function of tissues and organs, and body metabolism. The results showed that the length and mass of the digestive organs in the LRFI group were higher than those in the HRFI group as well as the chest width. Additionally, the key genes and metabolites regulating RFI in hens during the late laying phase were found to beADCY2, ADCY8, CCKAR, ACSS2, FABP1, FABP4, and LysoPI (18:2(9Z,12Z)/0:0) in the liver. The levels of AST, HDL-C and ACTH in the serum were considered candidate markers influencing RFI. By conducting a microbiome-metabolome association analysis, we have identified the dominant and beneficial microbial community in the gut of LRFI groups, such asOscillospirales,Ruminococcaceae, andButyricicoccaceae, which offers a theoretical basis for understanding how the gut microbiota regulates RFI. These results will provide a scientific basis for the molecular mechanism of RFI phenotypic variation in late laying hens.
Theta transcranial alternating current stimulation is not effective in improving working memory performance
Authors: Kasanov Dauren , Dorogina Olga , Mushtaq Faisal , Pavlov Yuri G. 
Published: March 2024

AbstractThere is an extensive body of research showing a significant relationship between frontal midline theta activity in the 4-8 Hz range and working memory (WM) performance. Transcranial alternating current stimulation (tACS) is recognized for inducing lasting changes in brain oscillatory activity. Across two experiments, we tested whether WM could be improved through tACS of dorsomedial prefrontal cortex and anterior cingulate cortex, by affecting executive control networks associated with frontal midline theta. In Experiment 1, following either a 20-minute verum or sham stimulation applied to Fpz-CPz at 1 mA and 6 Hz, 31 participants performed WM tasks, while EEG was recorded. The tasks required participants to either mentally manipulate memory items or retain them in memory as they were originally presented. No significant effects were observed in behavioral performance, and we found no change in theta activity during rest and task following stimulation. However, alpha activity during retention or manipulation of information in WM was less strongly enhanced during the delay period following verum stimulation as compared with sham. In Experiment 2 (N = 25), tACS was administered during the task in two separate sessions. Here, we changed the order of the stimulation blocks: a 25-minute task block was either accompanied first by sham stimulation and then by verum stimulation, or vice versa. Again, we found no improvements in WM through either tACS after-effects or online stimulation. Taken together, our results demonstrate that theta frequency tACS applied at the midline is not an effective method for enhancing WM.
Assessment of DNA quality for whole genome library preparation
Authors: Jansson Linda , Fagerholm Siri Aili , Börkén Emelie , Gynnå Arvid Hedén , Sidstedt Maja , Forsberg Christina , Ansell Ricky , Hedman Johannes , Tillmar Andreas 
Published: March 2024

AbstractIn recent years, more sophisticated DNA technologies for genotyping have enabled considerable progress in various fields such as clinical genetics, archaeogenetics and forensic genetics. DNA samples previously rejected as too challenging to analyze due to low amounts of degraded DNA can now provide useful information. To increase the chances of success with the new methodologies, it is crucial to know the fragment size of the template DNA molecules, and whether the DNA in a sample is mostly single or double stranded. With this knowledge, an appropriate library preparation method can be chosen, and the DNA shearing parameters of the protocol can be adjusted to the DNA fragment size in the sample. In this study, we first developed and evaluated a user-friendly fluorometry-based protocol for estimation of DNA strandedness. We also evaluated different capillary electrophoresis methods for estimation of DNA fragmentation levels. Next, we applied the developed methodologies to a broad variety of DNA samples processed with different DNA extraction protocols. Our findings show that both the applied DNA extraction method and the sample type affect the DNA strandedness and fragmentation. The established protocols and the gained knowledge will be applicable for future sequencing-based high-density SNP genotyping in various fields.
mRNA display in cell lysates enables identification of cyclic peptides targeting the BRD3 extraterminal domain
Authors: Hurd Catherine A. , Bush Jacob T. , Powell Andrew J. , Walport Louise J. 
Published: March 2024

ABSTRACTmRNA display is a powerful technology to screen libraries of >1012cyclic peptides against a protein target, enabling the rapid discovery of high affinity ligands. These cyclic peptides are particularly well suited to challenging protein targets that have been difficult to drug with small molecules. However, target choice can still be limited as screens are typically performed against purified proteins which often demands the use of isolated domains and precludes the use of aggregation-prone targets. Here, we report a method to perform mRNA display selections in mammalian cell lysates without the need for prior target purification, vastly expanding the potential target scope of mRNA display. We have applied the methodology to identify low to sub-nanomolar peptide binders for two targets; a NanoLuc subunit (LgBiT) and full-length bromodomain-containing protein 3 (BRD3). Our cyclic peptides for BRD3 were found to bind to the extraterminal (ET) domain of BRD3 and the closely related BRD proteins, BRD2 and BRD4. While many chemical probes exist for the bromodomains of BRD proteins, the ET domain is relatively underexplored, making these peptides valuable additions to the BRD toolbox.
How clinically relevant are prostate cancer cell lines? A comprehensive characterisation and multiomics comparison
Authors: Ahmed Zahra , Mosabbir Warda , Tandon Devansh , Pereira Snehal Pinto , Cheema Umber , Loizidou Marilena , Withington John , Moore Caroline , Okoli Uzoamaka , Heavey Susan 
Published: March 2024

AbstractCell line experiments arguably remain the most used tool in preclinical cancer research, despite their limitations. With almost 95% drugs entering human trials failing, and up to 90% preclinical research failing before even being tested in humans, we must shift the pre-clinical paradigm. A range of in silico, in vitro, in vivo and ex vivo approaches are gaining popularity, with the aim of potentially replacing cell line use. However, we cannot ignore the plethora of historical data from cell lines, nor write off their future use– especially within advanced bioengineered models. Therefore, we must question if and how cell lines hold clinical relevance. This study evaluates the clinical characteristics of 46 prostate cancer cell lines against worldwide data and investigates the biological features of seven cell lines in depth, comparing them to over 10,000 well characterised human cases from 24 studies in nine countries. Clinical features compared included age, ethnicity, Gleason grade, cancer type, treatment history and multiomics variables included mutations, copy number alterations, structural variants, microsatellite instability, mRNA and protein expression, and tumour mutational burden. We found that the most used cell lines accurately represent a minute proportion of prostate cancer patients. Furthermore, we recommend a pipeline for tailoring selection of clinically relevant cell lines with the ultimate aim of increasing the scientific methodology behind choosing a cell line.
Bioorthogonal labeling and enrichment of histone monoaminylation reveal its accumulation and regulatory function in cancer cell chromatin
Authors: Zhang Nan , Wu Jinghua , Hossain Farzana , Peng Haidong , Li Huapeng , Gibson Connor , Chen Min , Zhang Huan , Gao Shuaixin , Zheng Xinru , Wang Yongdong , Zhu Jiangjiang , Wang Jing J. , Maze Ian , Zheng Qingfei 
Published: March 2024

AbstractHistone monoaminylation (i.e., serotonylation and dopaminylation) is an emerging category of epigenetic mark occurring on the fifth glutamine (Q5) residue of H3 N-terminal tail, which plays significant roles in gene transcription. Current analysis of histone monoaminylation is mainly based on site-specific antibodies and mass spectrometry, which either lacks high resolution or is time-consuming. In this study, we report the development of chemical probes for bioorthogonal labeling and enrichment of histone serotonylation and dopaminylation. These probes were successfully applied for the monoaminylation analysis ofin vitrobiochemical assays, cells, and tissue samples. The enrichment of monoaminylated histones by the probes further confirmed the crosstalk between H3Q5 monoaminylation and H3K4 methylation. Finally, combining theex vivoandin vitroanalyses based on the developed probes, we have shown that both histone serotonylation and dopaminylation are highly enriched in tumor tissues that overexpress transglutaminase 2 (TGM2) and regulate the three-dimensional architecture of cellular chromatin.TOC
pSPObooster: a plasmid system to improve sporulation efficiency ofSaccharomyces cerevisiaelab strains
Authors: Loll-Krippleber Raphael , Jiang Yangyang Kate , Brown Grant W. 
Published: March 2024

AbstractCommonS. cerevisiaelab yeast strains derived from S288C have meiotic defects and therefore are poor sporulators. Here, we developed a plasmid system containing corrected alleles of theMKT1andRME1genes to rescue the meiotic defects and show that standard BY4741 and BY4742 strains containing the plasmid display faster and more efficient sporulation. The plasmid, pSPObooster, can be maintained as an episome and easily cured or stably integrated into the genome at a single locus. We demonstrate the use of pSPObooster in low- and high-throughput yeast genetic manipulations and show that it can expedite both procedures without impacting strain behavior.Take AwaypSPObooster contains corrected alleles orRME1andMKT1.pSPObooster can be maintained as an episome or integrated.pSPObooster increases sporulation efficiency by up to 13-fold.pSPObooster can be used to speed up high-throughput yeast strain engineering.
New understanding of nuclei spectra properties observed by the AMS-02 experiment
Authors: Dong Xu-Lin , Yao Yu-Hua , Guo Yi-Qing , Cui Shu-Wang 
Published: March 2024

The AMS-02 experiment has observed new properties of primary cosmic rays (CRs) categorized into two groups: He-C-O-Fe and Ne-Mg-Si-S, which are independent of CR propagation. In this study, we investigate the unexpected properties of these nuclei using a spatial propagation model. All nuclei spectra are accurately reproduced and separated into primary and secondary contributions. Our findings include: 1. Primary CR spectra are identical. 2. Our calculations align with AMS-02 results for primary-dominated nuclei within a 10% difference, but show significant discrepancies for the secondary-dominated nuclei. 3. The primary element abundance at around 200 GeV is presented and compared with previous solar and Galactic results. We hope that future DAMPE experiments can provide more experimental observational evidence to validate our model calculations.

Published by the American Physical Society
2024
How does market orientation affect disruptive green innovation? The mediating role of resource bricolage
Authors: Shang Tian-Tian , Dong Guang-Mao , Tian Min 
Published: March 2024

PurposeBased on the resource bricolage theory, we investigate the impact of proactive market orientation and responsive market orientation on firms’ disruptive green innovation. We also examine the impact of resource bricolage on disruptive green innovation and the mediating role of resource bricolage.Design/methodology/approachQuantitative data were collected from 232 firms in China. Structural equation modelling was used to test hypotheses.FindingsThe result show that proactive market orientation had positive effect on firm’s disruptive green innovation, whereas responsive market orientation had negative effect on firm’s disruptive green innovation. In addition, resource bricolage positively promotes firm’s disruptive green innovation. Resource bricolage played a mediating role between proactive market orientation and disruptive green innovation. Resource bricolage had a suppressing effect between responsive market orientation and disruptive green innovation.Originality/valueThis study makes up for the deficiency of the existing research on the relationship between market orientation and enterprise disruptive green innovation, improves the guidance mechanism of disruptive green innovation.
A hybrid approach for optimizing software defect prediction using a grey wolf optimization and multilayer perceptron
Authors: Mustaqeem Mohd , Mustajab Suhel , Alam Mahfooz 
Published: March 2024

PurposeSoftware defect prediction (SDP) is a critical aspect of software quality assurance, aiming to identify and manage potential defects in software systems. In this paper, we have proposed a novel hybrid approach that combines Grey Wolf Optimization with Feature Selection (GWOFS) and multilayer perceptron (MLP) for SDP. The GWOFS-MLP hybrid model is designed to optimize feature selection, ultimately enhancing the accuracy and efficiency of SDP. Grey Wolf Optimization, inspired by the social hierarchy and hunting behavior of grey wolves, is employed to select a subset of relevant features from an extensive pool of potential predictors. This study investigates the key challenges that traditional SDP approaches encounter and proposes promising solutions to overcome time complexity and the curse of the dimensionality reduction problem.Design/methodology/approachThe integration of GWOFS and MLP results in a robust hybrid model that can adapt to diverse software datasets. This feature selection process harnesses the cooperative hunting behavior of wolves, allowing for the exploration of critical feature combinations. The selected features are then fed into an MLP, a powerful artificial neural network (ANN) known for its capability to learn intricate patterns within software metrics. MLP serves as the predictive engine, utilizing the curated feature set to model and classify software defects accurately.FindingsThe performance evaluation of the GWOFS-MLP hybrid model on a real-world software defect dataset demonstrates its effectiveness. The model achieves a remarkable training accuracy of 97.69% and a testing accuracy of 97.99%. Additionally, the receiver operating characteristic area under the curve (ROC-AUC) score of 0.89 highlights the model’s ability to discriminate between defective and defect-free software components.Originality/valueExperimental implementations using machine learning-based techniques with feature reduction are conducted to validate the proposed solutions. The goal is to enhance SDP’s accuracy, relevance and efficiency, ultimately improving software quality assurance processes. The confusion matrix further illustrates the model’s performance, with only a small number of false positives and false negatives.
Brilliance in resilience: operations and supply chain management’s role in achieving a sustainable future
Authors: Holgado Maria , Blome Constantin , Schleper Martin C. , Subramanian Nachiappan 
Published: March 2024

PurposeThe purpose of this article is to discuss how the mastery of resilience in operations and supply chains plays a significant role in the transition to a more sustainable future. Furthermore, it is supposed to propose avenues for future research on operational and supply chain resilience, interacting with the sustainability literature in our field.Design/methodology/approachA conceptual review of resilience and sustainability themes within operations and supply chain management research is conducted. Reflections on the topic are informed by relevant literature published over the last decade.FindingsThe major conceptual contributions are threefold: (1) This article elaborates on the understanding of operational resilience and supply chain resilience concepts and reviews their respective primary research streams. (2) It proposes resilience as the missing element in the pursuit of excellence in organizations that want to contribute to a more sustainable future. (3) The article offers a research framework that provides a future research agenda at the intersection of resilience and sustainability in operations and supply chain management research.Originality/valueThe article highlights gaps in current research and illustrates further areas of research that need to be addressed to maximize the contribution of operations and supply chain management research in supporting practitioners to achieve a more sustainable future.
Exploring resistance in family policy transfer: a comparative analysis of the Czech Republic and South Korea
Authors: Gurín Martin 
Published: March 2024

PurposeFamily policy is an area where policy transfer has garnered a lot of attention lately. A growing body of research demonstrates policymakers' interest in and willingness to adopt foreign family policies. However, previous studies have tended to neglect the second mechanism of policy transfer: resistance. This manuscript aims to address this research gap by exploring both the willingness and resistance to policy transfer in Czech and Korean childcare and leave policies.Design/methodology/approachThis study employs a qualitative research design, incorporating structured expert interviews instrumental in in-depth thematic analysis.FindingsThe analysis shows that policymakers in both countries demonstrated interest and willingness to transfer family policies, albeit employing different strategies and to varying extents. Moreover, the two countries exhibited significant differences in resistance to family policy transfer, with resistance in the Czech Republic being more frequent and effective. Resistance is directed towards both forced and voluntary transfers, although it isn't always against transfers that require a paradigm change. Policy transfer and non-transfer can concurrently be perceived as threats.Originality/valueThe study concludes that integrating both policy transfer and resistance in the analyses helps to shed light on cross-national differences in family policy change and contributes to a more nuanced portrayal of the world of policy transfer in this policy field.
The relationship between the media and corruption: perspectives of Ghanaian justice and anti-corruption officials
Authors: Amagnya Moses Agaawena 
Published: March 2024

PurposeThe media is described as a fourth estate of the realm due to its ability to frame and shape discussions on governance and provide a stimulus for fighting corruption. But is the media really an effective tool for fighting corruption? This question arises due to the possibility of the media being used for propaganda, biased reporting and media owners’ and journalists’ engagement in corruption. The current study addresses the question by exploring the relationship between the media and corruption from the perspectives of Ghanaian justice and anti-corruption officials.Design/methodology/approachThe study adopts a qualitative approach by interviewing justice and anti-corruption officials across three administrative regions in Ghana.FindingsThe results show that while justice officials describe the media as a medium for accusing officials unjustifiably and exaggerating the scale of corruption, anti-corruption officials believe the media helps to fight corruption. In addition to uncovering and exposing public officials’ corruption, the media is also a double-edged sword characterised by intra-vigilance: the media hold “their own” (i.e. journalists fighting corruption) accountable through criticism and exposure of wrongdoings.Practical implicationsThe double-edged nature of the media can strengthen and enhance the fight against corruption because anti-corruption actors and journalists will be cautious as misjudgements or errors committed will not be overlooked or concealed by the media. Therefore, anti-corruption agencies in Ghana can collaborate with the media to uncover and expose corruption committed by public officials and even journalists or media owners.Originality/valueThis study is the first in Ghana to explore the relationship between the media and corruption from the perspectives of justice and anti-corruption officials. The approach, frameworks and methodology adopted in this study can be applied in similar studies in other countries on the African continent and beyond.
Analysis of tax compliance behavior of family businesses: combining social and psychology norms and legitimacy determinants
Authors: Belahouaoui Rida , Attak El Houssain 
Published: March 2024

PurposeThis study aims to analyze the tax compliance behavior of family firms by integrating social and psychological norms with legitimacy determinants, focusing specifically on the Moroccan context.Design/methodology/approachEmploying a qualitative research design, the study conducted semi-structured interviews with 30 chief executive officers (CEOs) of Moroccan family firms. The data were analyzed using thematic analysis to unravel the interplay between individual beliefs and societal norms.FindingsThe findings reveal a complex interplay between the personal norms of CEOs and chief financial officers (CFOs) and wider societal and cultural expectations, significantly influencing tax compliance behavior. The study identifies the multifaceted nature of tax compliance, which is shaped by personal ethics, family values and the dominant societal tax culture.Research limitations/implicationsThe research is limited by its qualitative approach and focus on Moroccan family businesses, which may not be generalizable to other contexts. Future studies could use a quantitative approach and expand to other geographical settings for a more comprehensive understanding.Practical implicationsInsights from the study can assist policymakers and tax authorities in developing culturally sensitive tax compliance strategies that resonate with family business values.Social implicationsThe research underscores the importance of considering sociocultural dimensions in tax compliance, fostering a more cooperative relationship between family businesses and tax authorities.Originality/valueThe study contributes a novel perspective by synthesizing social, psychological and legitimacy factors in understanding tax compliance in the unique context of family businesses.