bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional integrin subunits regulating cell–matrix interactions in the intervertebral disc
Authors: Gilchrist Christopher L. , Chen Jun , Richardson William J. , Loeser Richard F. , Setton Lori A. 
Published: February 2007

AbstractCellular interactions with the extracellular matrix are key factors regulating cell survival, differentiation, and response to environmental stimuli in cartilagenous tissues. Much is known about the extracellular matrix proteins in the intervertebral disc (IVD) and their variations with region, age, or degenerative state of the tissue. In contrast, little is known of the integrin cell surface receptors that directly bind to and interact with these matrix proteins in the IVD. In almost all tissues, these integrin‐mediated cell–matrix interactions are important for transducing environmental cues arising from mechanical stimuli, matrix degradation fragments, and cytokines into intracellular signals. In this study, cells from the nucleus pulposus and anulus fibrosus regions of porcine IVDs were analyzed via flow cytometry to quantify integrin expression levels upon isolation and after monolayer culture. Assays of cell attachment to collagens, fibronectin, and laminin were performed after functional blocking of select integrin subunits to evaluate the role of specific integrins in cell attachment. In situ distribution and co‐localization of integrins and laminin were also characterized. Results identify integrin receptors critical for IVD cell interactions with collagens (α1β1) and fibronectin (α5β1). Additionally, dramatic differences in cell–laminin interactions were observed between cells of the nucleus and anulus regions, including differences in α6 integrin expression, cell adhesion to laminin, and in situ pericellular environments. These findings suggest laminin–cell interactions may be important and unique to the nucleus pulposus region of the IVD. The results of this study provide new information on functional cell–matrix interactions in tissues of the IVD. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25: 829–840, 2007
Low‐level accelerations applied in the absence of weight bearing can enhance trabecular bone formation
Authors: Garman Russell , Gaudette Glenn , Donahue Leah‐Rae , Rubin Clinton , Judex Stefan 
Published: February 2007

AbstractHigh‐frequency whole body vibrations can be osteogenic, but their efficacy appears limited to skeletal segments that are weight bearing and thus subject to the induced load. To determine the anabolic component of this signal, we investigated whether low‐level oscillatory displacements, in the absence of weight bearing, are anabolic to skeletal tissue. A loading apparatus, developed to shake specific segments of the murine skeleton without the direct application of deformations to the tissue, was used to subject the left tibia of eight anesthesized adult female C57BL/6J mice to small (0.3 g or 0.6 g) 45 Hz sinusoidal accelerations for 10 min/day, while the right tibia served as an internal control. Video and strain analysis revealed that motions of the apparatus and tibia were well coupled, inducing dynamic cortical deformations of less than three microstrain. After 3 weeks, trabecular metaphyseal bone formation rates and the percentage of mineralizing surfaces (MS/BS) were 88% and 64% greater (p < 0.05) in tibiae accelerated at 0.3 g than in their contralateral controls. At 0.6 g, bone formation rates and mineral apposition rates were 66% and 22% greater (p < 0.05) in accelerated tibiae. Changes in bone morphology were evident only in the epiphysis, where stimulated tibiae displayed significantly greater cortical area (+8%) and thickness (+8%). These results suggest that tiny acceleratory motions — independent of direct loading of the matrix — can influence bone formation and bone morphology. If confirmed by clinical studies, the unique nature of the signal may ultimately facilitate the stimulation of skeletal regions that are prone to osteoporosis even in patients that are suffering from confinement to wheelchairs, bed rest, or space travel. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25: 732–740, 2007
Blocking Wnt/LRP5 signaling by a soluble receptor modulates the epithelial to mesenchymal transition and suppresses met and metalloproteinases in osteosarcoma Saos‐2 cells
Authors: Guo Yi , Zi Xiaolin , Koontz Zach , Kim Alison , Xie Jun , Gorlick Richard , Holcombe Randall F. , Hoang Bang H. 
Published: February 2007

AbstractWe previously reported the Wnt receptor low‐density lipoprotein receptor‐related protein 5 (LRP5) was frequently expressed in osteosarcoma (OS) tissue and correlated with metastasis and a lower disease‐free survival. Subsequent in vitro analysis revealed that dominant‐negative, soluble LRP5 (sLRP5) can reduce in vitro cellular invasion. In the current study, we examined the molecular mechanisms of blocking canonical Wnt signaling by sLRP5 in Saos‐2 osteosarcoma cells. Transfection of sLRP5 caused a marked up‐regulation of E‐cadherin in this cell line. This increase in E‐cadherin, seen primarily at the cell–cell contact borders, was associated with down‐regulation of Slug and Twist, transcriptional repressors which mediate cancer invasion and metastasis. In contrast, N‐cadherin, a mesenchymal marker, was reduced by sLRP5. In addition, blocking Wnt signaling by sLRP5 modulated other epithelial and mesenchymal markers (keratin 8 and 18, fibronectin), suggesting a reversal of epithelial–mesenchymal transition (EMT) seen during cancer progression. SLRP5 also reduced the expression of matrix metalloproteinase (MMP) 2 and 14, consistent with a decrease in invasive capacity. SLRP5 transfection decreased both Met expression and hepatocyte growth factor (HGF)‐induced cell motility. Taken together, these results support a role for Wnt/LRP5 signaling in invasiveness of a subset of OS cells. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:964–971, 2007
Oxidant and anti‐oxidant systems of synovial fluid from patients with knee post‐traumatic arthritis
Authors: Ostałowska Alina , Kasperczyk Sławomir , Kasperczyk Aleksandra , Słowińska Ludmiła , Marzec Marek , Stołtny Tomasz , Koczy Bogdan , Birkner Ewa 
Published: February 2007

AbstractIt has been suggested that patients with knee post‐traumatic arthritis (PA), associated or not to haemarthrosis (HA), display altered oxidant and anti‐oxidant systems in their synovial fluid. This study aimed to establish whether this is really the case. Synovial fluid samples were obtained by transdermal arthrocentesis from 69 patients with PA (36 of them had HA) and 22 control subjects. The activities of synovial fluid zinc‐copper superoxide dismutase (ZnCuSOD) and manganese superoxide dismutase (MnSOD) isoenzymes, catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione‐S‐transferase (GST) enzymes, and malondialdehyde (MDA) concentration and synovial fluid viscosity were measured in the study groups. Patients with PA had significantly increased activities of all antioxidant enzymes, except CAT, and MDA concentration than did the controls. However, synovial fluid viscosity was found to be decreased in the study group, mainly in the HA subgroup. Results suggest that excessive free radicals production may exist in synovial fluid of PA patients and may contribute to knee joint destruction. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25: 804–812, 2007
Fractalkine‐CX3CR1 axis regulates tumor cell cycle and deteriorates prognosis after radical resection for hepatocellular carcinoma
Authors: Matsubara Takeshi , Ono Takashi , Yamanoi Akira , Tachibana Mitsuo , Nagasue Naofumi 
Published: February 2007

AbstractBackground and ObjectivesFractalkine is the only CX3C chemokine, and its receptor, CX3CR1, is expressed on NK cells, CD8+ T cells, monocytes, and dendritic cells (DC). Although studies have reported that fractalkine regulates the host immune response, the roles of the fractalkine‐CX3CR1 axis in tumor biology and the clinical results of hepatocellular carcinoma (HCC) remain unknown.MethodsFractalkine and CX3CR1 expression in HCC were evaluated and compared with the clinicopathologic features, including tumor progression determined by proliferating cell nuclear antigen (PCNA) antibody and patient prognosis after surgery.ResultsTumors with high expression of both fractalkine and CX3CR1 had significantly fewer intra‐ and extrahepatic recurrences, a low PCNA labeling index (PCNALI), and different histological grades. Patients with tumors that expressed both had a significantly better prognosis in terms of disease‐free (DFS) and overall survival (OAS), and this finding was identified as one of the independent prognostic factors in the multivariate analysis.ConclusionsOur results suggest that the fractalkine‐CX3CR1 axis plays a pivotal role in the prognosis of patients with HCC, which might arise from the known modulation of the host immune response, and that of the cell cycle in HCC. J. Surg. Oncol. 2007;95:241–249. © 2007 Wiley‐Liss, Inc.
Mortality following liver resection in US medicare patients: Does the presence of a liver transplant program affect outcome?
Authors: Dixon Elijah , Schneeweiss Sebastian , Pasieka Janice L. , Bathe Oliver F. , Sutherland Francis , Doig Christopher 
Published: February 2007

AbstractBackgroundHepatic resection is a complicated procedure, at times associated with significant morbidity. Liver transplantation programs may improve outcomes following resective liver surgery at the institutional level by a number of means, including: availability of ancillary services and personnel, specialized critical care, and added surgical expertise.ObjectivesTo determine if the presence of a liver transplant program at a center improves outcomes following hepatic resection when compared to centers without an associated liver transplant program.MethodsUsing data from the national Medicare claims database, 30‐day mortality following all hepatic resections performed over a 2‐year period (1999, 2000) were studied. Regression techniques were used to assess the relationship between mortality at centers with an associated liver transplant program in comparison to those without, while controlling for potential confounding factors.ResultsThe proportion of patients dying within 30 days among 4,661 patients that underwent hepatic resection was 6.65%. Factors that did increase the risk of dying after hepatic resection included: urgent or emergent surgery (vs. elective), primary liver cancer (vs. metastatic), male sex, increasing comorbidity score, low hospital volume, and extent of surgery. The presence of a liver transplant program within a center was not associated with any improvement in mortality.ConclusionAt an institutional level, the presence of a liver transplant program was not associated with decreased 30‐day mortality following hepatic resection. J. Surg. Oncol. 2007;95:194–200. © 2007 Wiley‐Liss, Inc.
Expression of tissue inhibitor of metalloproteinase‐3 (TIMP‐3) and its prognostic significance in resected non‐small cell lung cancer
Authors: Mino Nobuya , Takenaka Kazumasa , Sonobe Makoto , Miyahara Ryo , Yanagihara Kazuhiro , Otake Yosuke , Wada Hiromi , Tanaka Fumihiro 
Published: February 2007

AbstractBackground and ObjectivesTissue inhibitor of metalloproteinase‐3 (TIMP‐3) inhibits the activity of metalloproteinases that play important roles in development and progression of malignant tumors. We conducted a retrospective study of TIMP‐3 expression in resected non‐small cell lung cancer (NSCLC).MethodsTIMP‐3 expression was examined immunohistochemically in primary tumor specimens from 143 patients who underwent complete resection for NSCLC. Correlations between TIMP‐3 expression grade and tumor histology, TNM classification, MMP‐2 and MMP‐9 expression grade, VEGF expression grade, intra‐tumoral microvessel density, proliferative index, apoptosis index, and prognosis were analyzed.ResultsTIMP‐3 expression was low in 40, moderate in 71, and high in 32 patients. Higher TIMP‐3 expression was seen in squamous cell carcinoma than in adenocarcinoma (P = 0.001), and reduced TIMP‐3 expression was significantly associated with nodal involvement (P = 0.016) and advanced pathologic stage (P = 0.036). MMP‐2 expression was reduced along with enhanced TIMP‐3 expression (P = 0.010). The 5‐year overall survival rates of low, moderate, and high TIMP‐3 patients were 53, 64, and 84%, respectively (P = 0.037). Multivariate analysis confirmed that enhanced TIMP‐3 expression was an independent factor for a favorable prognosis (P = 0.037).ConclusionsTIMP‐3 expression status was significantly correlated with pathologic stage and nodal involvement, and was an independent prognostic factor in resected NSCLC. J. Surg. Oncol. 2007;95:250–257. © 2007 Wiley‐Liss, Inc.
Family ties: Managers can benefit from personal lives
Authors: Ruderman Marian N. , Graves Laura M. , Ohlott Patricia J. 
Published: February 2007

AbstractThe mantra that managers, to be effective, must be totally committed to work while disregarding their families and other aspects of their personal lives in order to be effective is coming into question. New research shows that attentiveness to family is tied to better performance at work.
An alternative approach to the image reconstruction for parallel data acquisition in MRI
Authors: Bauer Frank , Kannengiesser Stephan 
Published: February 2007

AbstractMagnetic resonance imaging with parallel data acquisition requires algorithms for reconstructing the patient's image from a small number of measured k‐space lines.In contrast to well‐known algorithms like SENSE and GRAPPA and its flavours we consider the problem as a non‐linear inverse problem. Fast computation algorithms for the necessary Fréchet derivative and reconstruction algorithms are given. Copyright © 2007 John Wiley & Sons, Ltd.
Obturator neuropathy complicating elective laparoscopic tubal occlusion
Authors: Jirsch Jeffrey D. , Chalk Colin H. 
Published: February 2007

AbstractIsolated obturator neuropathy is rare. We report a woman who developed a severe obturator neuropathy from electrocautery during elective laparoscopic tubal ligation. This complication has not previously been described in association with the procedure, and the potential etiological role of an underrecognized anatomical variant, in which an accessory obturator nerve is present, is discussed. Muscle Nerve, 2007
Successful treatment of a child with late onset T‐cell post‐transplant lymphoproliferative disorder/lymphoma
Authors: Williams Kirsten M. , Higman Meghan A. , Chen Allen R. , Schwartz Cindy L. , Wharam Moody , Colombani Paul , Arceci Robert J. 
Published: February 2007

AbstractWe report a novel regimen for refractory post‐transplant T‐cell lymphoma (PTL). Our patient presented with non‐Epstein–Barr virus (EBV) related, T‐cell post‐transplant lymphoproliferative disease (PTLD) 3.5 years after liver transplantation. Initially diagnosed as polyclonal PTLD, the disease progressed to a monoclonal, T‐cell PTL that was refractory to several chemotherapy regimens but responded to a regimen consisting of fludarabine, cyclophosphamide, cytarabine, and alemtuzumab. Consolidation therapy included high‐dose chemotherapy, autologous hematopoietic stem cell rescue, and radiation therapy. She remains in remission 2.5 years later. T‐cell PTL is a rare disease with a poor prognosis; this regimen provides a novel, potentially curative approach for its treatment. Pediatr Blood Cancer 2008;50:667–670. © 2007 Wiley‐Liss, Inc.
Supramolecular command surfaces for liquid crystal alignment
Authors: Hoogboom Johan , Elemans Johannes AAW , Rasing Theo , Rowan Alan E , Nolte Roeland JM 
Published: February 2007

AbstractDevices based on liquid crystal display technology, ranging from watches to flat panel displays, have proliferated over the past few decades and can now be found in all conceivable aspects of everyday life. It is surprising that a crucial part in the construction of such displays, namely the alignment layer, which dictates the surface alignment of mesogenic molecules, relies essentially on a mechanical rubbing process which was invented 100 years ago. In this paper, recent developments in the construction of alignment layers (also called command layers) based on the self‐assembly of molecular and macromolecular components are discussed. Two topics are highlighted: (i) tuneable layers formed by hierarchical assembly of siloxane oligomers and phthalocyanine dyes on indium‐tin‐oxide surfaces and (ii) command layers formed by self‐assembly of porphyrin trimers. Copyright © 2007 Society of Chemical Industry
Sense of coherence and distress in cancer patients and their partners
Authors: Gustavsson‐Lilius Mila , Julkunen Juhani , Keskivaara Pertti , Hietanen Päivi 
Published: February 2007

AbstractStrong sense of coherence (SOC) is assumed to promote and protect health in stressful situations, such as a serious illness. There is, however, surprisingly little research‐based discussion on the SOC–distress association in cancer patients and especially in their partners. The aim of this study was to clarify these issues.The associations between SOC, depression, and anxiety were studied in 123 cancer couples. Data were collected with self‐report questionnaires at the time of diagnosis, 8 and 14 months later. The predictors of follow‐up distress and possible mediators of the cross‐lagged longitudinal data were analysed with SEM.No gender differences in the patients' study variables were found, but the female partners displayed more distress symptoms than their male counterparts. The results supported the SOC theory. Strong SOC alleviated the development of distress. In addition, patient SOC tended to strengthen during the follow‐up. No direct crossover between baseline SOC and follow‐up distress was found. However, all patient and partner variables at the 14‐month follow‐up were related to each other, but not at baseline. This could indicate a gradual crossover process of the shared experience. Special attention in clinical practice should be given to the psychological well‐being of cancer patients' partners, especially female partners. Copyright © 2007 John Wiley & Sons, Ltd.
Environmentally friendly approach for nonlithographic nanostructuring of materials
Authors: Tiginyanu I. M. , Monaico E. , Albu S. , Ursaki V. V. 
Published: February 2007

AbstractSelf‐organized quasi‐ordered two‐dimensional hexagonal arrays of pores with diameters as low as 70 nm in n‐InP substrates subjected to anodic etching in aqueous solution of NaCl are reported. We show that proper periodic modulation of the applied potential with time allows one to reach 3D nanostructuring of the material. Anodization in salty water proves to be a cost‐effective and environmentally‐friendly tool for spatial nanostructuring of materials and nonlithographic manufacturing of semiconductor nanotemplates for nanofabrication. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Elucidation of the in vitro metabolic profile of stable isotope labeled BAL19403 by accurate mass capillary liquid chromatography/quadrupole time‐of‐flight mass spectrometry and isotope exchange
Authors: Wind Mathias , Gebhardt Klaus , Grunwald Helge , Spickermann Jochen , Donzelli Massimiliano , Kellenberger Laurenz , Muller Marc , Fullhardt Pascal , Schmitt‐Hoffmann Anne , Schleimer Michael 
Published: February 2007

AbstractThe in vitro metabolic pattern of BAL19403, a novel macrolide antibiotic, was investigated by capillary liquid chromatography/quadrupole time‐of‐flight mass spectrometry (LC/QTOF‐MS) in incubations with human microsomes. For the elucidation of the metabolic pathway, BAL19403 labeled with four deuterium atoms (D4) was used, and detection of metabolites performed using mixtures of the unlabeled (H4) BAL19403 and its D4 analogue (1:1) as substrate. All metabolites appeared with similar chromatographic behavior. MS/MS spectra of BAL19403 and its metabolites are dominated by non‐informative fragment ions. Therefore, the structure of the metabolites was elucidated mainly by accurate mass measurements with subsequent proposals of elemental compositions. Main biotransformations were N‐demethylation, lactone ring hydrolysis, and oxidation. Additionally, N‐dealkylation of the aromatic moiety was identified. This dealkylation results not only in formation of an aldehyde, according to the classical pathway, but also in formation of the corresponding alcohol and carboxylic acid. Final elucidation of their structures was possible, since this dealkylation takes place vicinal to the deuterium‐labeled part of BAL19403 and interferes with D/H exchange. The degree of D/H exchange, determined by analysis of the metabolite isotopic pattern, was used to elucidate the adjacent functional group. Copyright © 2007 John Wiley & Sons, Ltd.
Predicting joint frequency distributions of depth and velocity for instream habitat assessment
Authors: Schweizer Steffen , Borsuk Mark E. , Jowett Ian , Reichert Peter 
Published: February 2007

AbstractStream ecosystem structure and function are strongly influenced by patterns of velocity and depth. Simple methods for predicting the spatial distributions of these two variables, as functions of one‐dimensional reach and discharge characteristics, have been recently reported in the literature. These studies have provided valuable insight into the fundamental factors influencing stream behaviour and represent a practical alternative to multi‐dimensional hydrodynamic models. However, these previous studies have handled velocity and depth separately, while there is evidence that meso‐habitats and stream biota are associated with distinct combinations of the two variables. Therefore, we used survey data from 92 stream reaches in New Zealand to develop a model for the joint distribution of depth and velocity. We found that, for each reach, the bivariate distribution of relative velocity and relative depth could be described by a mixture of two end‐member distributions, one bivariate normal and the other bivariate lognormal, each with fixed parameters. The relative contribution of each shape for a particular reach at a particular discharge could then be related to the reach mean Froude number, the reach mean relative roughness, and the ratio of the survey discharge to the mean discharge. As these inputs can be readily estimated for changed channel morphology, our model should provide a useful approach for linking river rehabilitation strategies to hydraulics and ecology. Copyright © 2007 John Wiley & Sons, Ltd.
Adsorption of genetically engineered proteins studied by time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS). Part B: hierarchical cluster analysis (HCA)
Authors: Suzuki Noriaki , Sarikaya Mehmet , Ohuchi Fumio S. 
Published: February 2007

AbstractIn Part A, we adopted principal component analysis (PCA) for the analysis of TOF‐SIMS data to assess the binding specificity of GBP‐1 to metallic Au, Ag and Pd. Within a given set of data, PCA aids in the interpretation of the TOF‐SIMS spectra by capitalizing on the differences from one spectrum to another. In Part B, we introduce another multivariate statistical method called ‘hierarchical cluster analysis (HCA)’, where visualization of the similarity and difference in data is readily observed, from which a variety of adsorption conditions of GBP‐1 were characterized. Copyright © 2007 John Wiley & Sons, Ltd.