bluuebunny/crossref_metadata_2025_split huggingface dataset:
ISTH bleeding assessment tool and platelet function analyzer in children with mild inherited platelet function disorders
Authors: Alhaj Dana , Hagedorn Nikola , Cuntz Franziska , Reschke Madlen , Schuldes Joerg , Ruthenberg Juliane , Bakchoul Tamam , Greinacher Andreas , Holzhauer Susanne 
Published: March 2024

AbstractObjectivesTo evaluate the diagnostic performance of platelet function analyzer (PFA) and The International Society on Thrombosis and Hemostasis bleeding‐assessment‐tool (ISTH‐BAT) in detecting mild inherited platelet function disorders (IPFDs) in children with suspected bleeding disorders.MethodsProspective single‐center diagnostic study including consecutive patients <18 years with suspected bleeding disorder and performing a standardized workup for platelet function defects including ISTH‐BAT, PFA, platelet aggregation testing, blood smear‐based immunofluorescence, and next‐generation sequencing‐based genetic screening for IPFDs.ResultsWe studied 97 patients, of which 34 von Willebrand disease (VWD, 22 type‐1, 11 type‐2), 29 IPFDs (including delta−/alpha‐storage pool disease, Glanzmann thrombasthenia, Hermansky–Pudlak syndrome) and 34 with no diagnosis. In a model combining PFA‐adenosine diphosphate (ADP), PFA‐epinephrine (EPI), and ISTH‐BAT overall performance to diagnose IPFDs was low with area under the curves of 0.56 (95% CI 0.44, 0.69) compared with 0.84 (95% CI 0.76, 0.92) for VWD. Correlation of PFA‐EPI/‐ADP and ISTH‐BAT was low with 0.25/0.39 Spearman's correlation coefficients. PFA were significantly prolonged in patients with VWD and Glanzmann thrombasthenia. ISTH‐BAT‐scores were only positive in severe bleeding disorders, but not in children with mild IPFDs or VWD.ConclusionNeither ISTH‐BAT nor PFA or the combination of both help diagnosing mild IPFDs in children. PFA is suited to exclude severe IPFDs or VWD and is in this regard superior to ISTH‐BAT in children.
Beyond a single temperature threshold: Applying a cumulative thermal stress framework to plant heat tolerance
Authors: Cook Alicia M. , Rezende Enrico L. , Petrou Katherina , Leigh Andy 
Published: March 2024

AbstractMost plant thermal tolerance studies focus on single critical thresholds, which limit the capacity to generalise across studies and predict heat stress under natural conditions. In animals and microbes, thermal tolerance landscapes describe the more realistic, cumulative effects of temperature. We tested this in plants by measuring the decline in leaf photosynthetic efficiency (FV/FM) following a combination of temperatures and exposure times and then modelled these physiological indices alongside recorded environmental temperatures. We demonstrate that a general relationship between stressful temperatures and exposure durations can be effectively employed to quantify and compare heat tolerance within and across plant species and over time. Importantly, we show how FV/FM curves translate to plants under natural conditions, suggesting that environmental temperatures often impair photosynthetic function. Our findings provide more robust descriptors of heat tolerance in plants and suggest that heat tolerance in disparate groups of organisms can be studied with a single predictive framework.
Nonlinear life table response experiment analysis: Decomposing nonlinear and nonadditive population growth responses to changes in environmental drivers
Authors: O'Connell Ryan D. , Doak Daniel F. , Horvitz Carol C. , Pascarella John B. , Morris William F. 
Published: March 2024

AbstractLife table response experiments (LTREs) decompose differences in population growth rate between environments into separate contributions from each underlying demographic rate. However, most LTRE analyses make the unrealistic assumption that the relationships between demographic rates and environmental drivers are linear and independent, which may result in diminished accuracy when these assumptions are violated. We extend regression LTREs to incorporate nonlinear (second‐order) terms and compare the accuracy of both approaches for three previously published demographic datasets. We show that the second‐order approach equals or outperforms the linear approach for all three case studies, even when all of the underlying vital rate functions are linear. Nonlinear vital rate responses to driver changes contributed most to population growth rate responses, but life history changes also made substantial contributions. Our results suggest that moving from linear to second‐order LTRE analyses could improve our understanding of population responses to changing environments.
Predictors of change in relationship satisfaction among Black postpartum mothers
Authors: Weber Danielle M. , Bryant Chalandra M. , Williamson Hannah C. , Mussa Kadija , Lavner Justin A. 
Published: March 2024

AbstractThe transition to parenthood can be a challenging time for the relationships of new parents and result in declines in relationship satisfaction. Although a robust literature has identified characteristics that predict changes in relationship satisfaction during this period, the relationships of Black mothers postpartum remain understudied. To address this gap, we examined a set of relational, individual, and external characteristics as predictors of relationship satisfaction trajectories over the first four months postpartum. First‐time Black mothers (N = 93, 22.6% married, 52.7% cohabiting, 24.7% not cohabiting) reported on relational, individual, and external characteristics at 1 week postpartum and their relationship satisfaction at 1, 8, and 16 weeks postpartum. Mothers who reported more commitment and partner support were higher in initial satisfaction, as were mothers who were married or cohabiting with a partner (relative to mothers who were not cohabiting with their partner). Mothers with clinically significant depressive symptoms at 1 week postpartum had lower initial relationship satisfaction than mothers without clinically significant depressive symptoms. Mothers' sleep difficulties and experiences of racial discrimination were associated with changes in relationship satisfaction over time; mothers experiencing more sleep difficulties and racial discrimination experienced larger declines in satisfaction. These findings offer new insights into risk and protective factors associated with relationship satisfaction among Black mothers during the early postpartum period and can inform multicomponent interventions to enhance their relationship functioning.
Microwave treatment affects the storage stability of rice: the influence on the structure and properties of starch
Authors: Liu Shiwei , Hassan Nora Ali , He Jie , Chen Xinli , Liu Guangxin , Wang Wei , Hu Aijun , Zheng Jie 
Published: March 2024

SummaryMicrowave (MW) is a non‐heating, environmental‐friendly method used to change starches. Rice was treated at various powers (2, 4, 6 and 8 W g−1). In vitro glycaemic index, lipase activity (LA), fatty acid value (FAV) and reducing sugars (RS) were determined in rice and linked to changes in rice starch structure and thermal properties caused by microwave, which were altered by an increase in power, which reduce values. The findings of pasting viscosity among microwave rice starch (MS) at four powers revealed decreased values when powers were increased. MS treated at power 2 W g−1 produced the lowest pasting temperature, which was required to gelatinise starch. A loss of granular crystallinity in treated samples was confirmed by XRD and FTIR, particularly 33.07%, 32.51%, 20.94% and 20.13% respectively. And the XRD patterns of MS were not changed. Microwave rice starch allowed the formation of the structural and thermal properties of rice starch. In vitro glycaemic index revealed that samples treated in 6 and 8 W g−1 showed significantly higher rapid hydrolyses than MS treated in 2 and 4 W g−1. The utilisation of microwaves had an additional advantage in improving the storage stability of rice and the structure and properties of rice starch.
Prostate cancer and liquid biopsies: Clinical applications and challenges
Authors: Urabe Fumihiko , Sumiyoshi Takayuki , Tashiro Kojiro , Goto Takayuki , Kimura Takahiro , Kobayashi Takashi 
Published: March 2024

AbstractLiquid biopsy has emerged as a valuable and minimally invasive tool for real‐time detection of clinically actionable abnormalities across various cancer types. Its applicability is particularly compelling in the realm of prostate cancer, where novel therapeutic agents, including those targeting DNA repair systems, are under development. Despite these advancements, challenges persist in effectively screening for prostate cancer, enhancing risk stratification, and determining optimal approaches for treating advanced disease. Consequently, there is a pressing need for improved biomarkers to aid clinicians in decision‐making within these contexts. Cell‐free DNA and extracellular vesicle analysis have demonstrated promise in diagnosis, prognostication, assessment of treatment responses, and identification of emerging mechanisms of resistance. Nevertheless, obstacles must be addressed before liquid biopsies can be integrated into routine clinical practice. These challenges encompass preanalytical considerations such as sample collection and storage, methods of extracellular vesicle isolation and enrichment, and the need for enhanced interpretation of generated sequencing data. This review provides a comprehensive overview of current clinical opportunities in managing prostate cancer through blood‐based liquid biopsy, highlighting the progress made, and acknowledging the challenges that remain. Additionally, we discuss the next steps required for the effective implementation of liquid biopsies in guiding personalized treatment strategies for prostate cancer.
SfREPAT38, a pathogen response gene (REPAT), is involved in immune response of Spodoptera frugiperda larvae through mediating Toll signalling pathway
Authors: Wang Yuxue , Mbiza Natasha Isabel Tanatsiwa , Liu Ting , Wang Yi , Zhang Yi , Luo Xincheng , Chu Longyan , Li Jianping , Yang Yazhen , Wang Xiangping , Zhang Jianmin , Yu Yonghao 
Published: March 2024

AbstractREPAT (response to pathogen) is an immune‐associated gene family that plays important roles in insect immune response to pathogens. Although nine REPAT genes have been identified in Spodoptera frugiperda (Lepidoptera: Noctuidae) currently, their functions and mechanisms in the immune response to pathogens still remain unclear. Therefore, SfREPAT38, a pathogen response gene (REPAT) of S. frugiperda, was characterised and its function was analysed. The results showed that SfREPAT38 contains a signal peptide and a transcription activator MBF2 (multi‐protein bridging factor 2) domain. Quantitative real‐time polymerase chain reaction analysis showed that SfREPAT38 was highly expressed in the sixth‐instar larvae (L6) and was the highest in expression in the midgut of L6. We found that the expression of SfREPAT38 could be activated by challenge with four microbial pathogens (Bacillus thuringiensis, Metarhizium anisopliae, Spodoptera exigua nuclearpolyhedrosis and Escherichia coli), except 12 h after E. coli infection. Furthermore, the SfREPAT38 expression levels significantly decreased at 24, 48 and 72 h after SfREPAT38 dsRNA injection or feeding. Feeding with SfREPAT38 dsRNA significantly decreased the weight gain of S. frugiperda, and continuous feeding led to the death of S. frugiperda larvae from the fourth day. Moreover, SfREPAT38 dsRNA injection resulted in a significant decrease of weight gain on the fifth day. Silencing SfREPAT38 gene down‐regulated the expression levels of immune genes belonging to the Toll pathway, including SPZ, Myd88, DIF, Cactus, Pell and Toll18W. After treatment with SfREPAT38 dsRNA, S. frugiperda became extremely sensitive to the B. thuringiensis infection, and the survival rate dramatically increased, with 100% mortality by the eighth day. The weight of S. frugiperda larvae was also significantly lower than that of the control groups from the second day onwards. In addition, the genes involved in the Toll signalling pathway and a few antibacterial peptide related genes were down‐regulated after treatment. These results showed that SfREPAT38 is involved in the immune response of S. frugiperda larvae through mediating Toll signalling pathway.
Halo‐hydromorphism alters nitrogen fertilization responses of tall wheatgrass pastures: Capture and use of resources, tiller dynamics and forage production
Authors: Fina Federico , Bertram Nicolás , Gatti María Laura , Di Bella Carla E. , Grimoldi Agustín A. , Berone Germán D. 
Published: March 2024

AbstractHalo‐hydromorphism limits productivity in approximately 100 million hectares worldwide. Tall wheatgrass (Thinopyrum ponticum) is a species widely used in these environments for its seeding potential, being the addition of nitrogen a considered technological tool to increase forage quality and production. The objective of the study was to determine the impact of nitrogen fertilization on the capture and use of resources (radiation, water and nitrogen) in a cool season perennial sward growing in contrasting halo‐hydromorphic conditions. Cultivated pastures from three independent sites were used. Sites were described according to the degree of halo‐hydromorphism using soil salinity and water table attributes (salinity and depth) as environmental indicators: low HHM site [electrical conductivity (EC1:2.5) 0.97 dS/m; water table salinity 2.03 dS/m; depth 85 cm], intermediate HHM site (EC1:2.5 3.86 dS/m; water table salinity 7.40 dS/m; depth 134 cm) and high HHM site (EC1:2.5 4.49 dS/m; water table salinity 7.85 dS/m; depth 31 cm). At each site, a late spring regrowth (~750°Cd) was studied by applying two treatments (n = 5): without (N0) and nitrogen fertilization (N150; 150 kg/ha of nitrogen in the form of urea). The response of tall wheatgrass to nitrogen fertilization in halo‐hydromorphic conditions depends on soil salinity and water table attributes. N150 treatments production was twice as high as in N0 in low HHM and intermediate HHM environments (from 1750 to 3500 kgDM/ha and from 1080 to 1985 kgDM/ha, respectively). Meanwhile, in high HHM conditions, forage production was only 40% higher when nitrogen was added (from 625 to 870 kgDM/ha). In low HHM the higher N150 production was related to tiller density and size, whereas in intermediate HHM and high HHM was linked only to tiller size. In N150 treatments, the nitrogen nutrition index was negatively affected with the increase in HHM conditions (0.77, 0.62 and 0.55 for low HHM, intermediate HHM and high HHM, respectively). Instead, nitrogen nutrition index of N0 was similar in all the environments (~0.42). In N150, forage production capacity analysed in terms of radiation and water use efficiency (RUE and WUE, respectively) was similar in low HHM and intermediate HHM environments (RUE ~0.81 gDM/Mj and WUE ~13 kgDM/mm). These findings emphasize the importance of conducting analyses based on resource use and capture to understand productive responses to the increase in growth‐limiting factors. Furthermore, they contribute to the identification of environments suitable for nitrogen fertilization.
Systolic and diastolic blood pressure time in target range and cardiovascular outcomes in patients with hypertension and pre‐frailty or frailty status
Authors: Zhu Jiajia , Yang Kun , Liu Wenxian 
Published: March 2024

AbstractIn patients with hypertension and pre‐frailty or frailty, the influence of systolic (SBP) and diastolic blood pressure (DBP) time in target range (TTR) on clinical outcomes is unclear. Thus, we conducted a post hoc analysis of the Systolic Blood Pressure Intervention Trial (SPRINT). Classifying 4208 participants into frail and non‐frail groups using a frailty index, the study calculated blood pressure time in target range (BP‐TTR) for the first three months using the Rosendaal method. The primary endpoint included a composite of nonfatal myocardial infarction (MI), acute coronary syndromes, stroke, acute decompensated heart failure (ADHF), and cardiovascular death. Relationships between BP‐TTR and outcomes were analyzed using Kaplan‐Meier curves, Cox models, and restricted cubic spline curves, with subgroup analysis for further insights. In a median follow‐up of 3.17 years, primary outcomes occurred in 6.7% of participants. Kaplan‐Meier analysis showed that a lower systolic blood pressure time in target range (SBP‐TTR) (0%–25%) correlated with an increased cumulative incidence of the primary outcome (p < .001), nonfatal MI (P = .021), stroke (P = .004), and cardiovascular death (P = .002). A higher SBP‐TTR (75%–<100%) was linked to a reduced risk of these outcomes. The restricted cubic spline (RCS) curve revealed a linear association between SBP‐TTR and the primary outcome (non‐linear P = .704). Similar patterns were observed for diastolic blood pressure time in target range (DBP‐TTR). Subgroup analysis showed that the protective effect of higher SBP‐TTR was less pronounced at low DBP‐TTR levels (P for interaction = .023). In conclusion, this study highlights the importance of maintaining BP within the target range to mitigate cardiovascular risks in this population.
Identification of fish specimens of the Tocantins River, Brazil, using DNA barcoding
Authors: Lima Renato Corrêia , de Lima Sabrina Rufino , Rocha Marcelo Salles , dos Anjos Hélio Daniel Beltrão , Dantas Yasmin Catarina Alves , Benites Ignácio Davi Navegante , Queiroz Cleonilde da Conceição Silva , Fraga Elmary da Costa , Batista Jacqueline da Silva 
Published: March 2024

AbstractThe fish fauna of the Tocantins River possesses many endemic species; however, it is little studied in molecular terms and is quite threatened by the construction of several hydroelectric dams. Therefore, the objective of this study was to identify the ichthyofauna of the Tocantins River using DNA barcoding. For this, collections were carried out in five points of this river, which resulted in the capture of 725 individuals from which partial sequences of the cytochrome oxidase subunit I (COI) gene were obtained for genetic analysis. A total of 443 haplotypes were recovered with the mean intraspecific K2P genetic distance of 1.82%. Altogether, 138 species were identified based on morphological criteria, which was a quantity that was much lower than that indicated by the four molecular methods (assemble species by automatic partitioning [ASAP], barcode index number [BIN], generalized mixed Yule coalescent (GMYC), and Bayesian Poisson tree processes [bPTP]) through which 152–157 molecular entities were identified. In all, 41 unique BINs were obtained based on the data generated in the BOLDSystems platform. According to the result indicated by ASAP (species delimitation approach considered the most appropriate in the present study), there was an increase of 17 molecular entities (12.32%), when compared to the number of species identified through their morphological criteria, as it can show cryptic diversity, candidates for new species, and misidentifications. There were 21 incongruities indicated between the different identification approaches for species. Therefore, it is suggested that these taxonomic problems be cautiously evaluated by experts to solve such taxonomic issues.
The effectiveness of non‐pharmacological interventions on reducing pain in patients undergoing bone marrow aspiration and biopsy: A systematic review and meta‐analysis of randomized controlled trials
Authors: Li Chengyang , Zhang Xiao , Zhuang Xueting , Zhang Kun , Huang Qiyuan , Ge Song , Wu Yong , Hu Rong 
Published: March 2024

AbstractBackgroundPatients often consider bone marrow aspiration and biopsy to be one of the most painful medical procedures. The effectiveness of non‐pharmacological interventions to reduce pain during bone marrow aspiration and biopsy remains unclear.AimTo synthesize existing evidence regarding the effectiveness of non‐pharmacological interventions in mitigating procedural pain among patients undergoing bone marrow aspiration and biopsy.DesignA systematic review and meta‐analysis of randomized controlled trials.MethodsSix electronic databases, including PubMed, EMBASE, CINAHL, PsycINFO, Cochrane Library and Web of Science were searched from inception to July 15, 2023. The risk of bias was assessed using the Cochrane Risk of Bias Tool Version 2.0. Meta‐analysis was conducted using STATA 16. The certainty of the evidence was assessed by the GRADE approach.ResultsThis meta‐analysis included 18 studies derived from 17 articles involving a total of 1017 participants. The pooled results revealed statistically significant pain reduction effects using distraction (SMD: −.845, 95% CI: −1.344 to −.346, p < .001), powered bone marrow biopsy system (SMD: −.266, 95% CI: −.529 to −.003, p = .048), and acupoint stimulation (SMD: −1.016, 95% CI: −1.995 to −.037, p = .042) among patients undergoing bone marrow aspiration and biopsy. However, the pooled results on hypnosis (SMD: −1.228, 95% CI: −4.091 to 1.515, p = .368) showed no significant impact on pain reduction. Additionally, the pooled results for distraction did not demonstrate a significant effect on operative anxiety (MD: −2.942, 95% CI: −7.650 to 1.767, p = .221).ConclusionsDistraction, powered bone marrow biopsy system and acupoint stimulation are effective in reducing pain among patients undergoing bone marrow aspiration and biopsy.Patient or Public ContributionNot applicable.Relevance to Clinical PracticeThis meta‐analysis highlights the effectiveness of distraction, powered bone marrow biopsy system and acupoint stimulation for reducing pain in patients undergoing bone marrow biopsy. Healthcare professionals should consider integrating these interventions into pain management practices for these patients.Registration(PROSPERO): CRD42023422854.
Nursing practices to optimise rheumatic fever prevention in a high‐risk country: An integrative review
Authors: Murray Ruby , Mowat Rebecca , Foster Mandie Jane , Blamires Julie 
Published: March 2024

AbstractBackgroundNew Zealand is one of the last high‐income countries in the world experiencing significant rates of rheumatic fever. Nurses play a crucial role in rheumatic fever prevention; however, little is understood as to how nurses can best achieve this.AimTo explore nursing practices that optimise rheumatic fever prevention.DesignAn integrative review.MethodsFour electronic databases (CINAHL, SCOPUS, Medline via, and Ovid) were searched for peer‐reviewed empirical articles published from 2013 to 2023. Grey literature (guidelines/reports) was also sourced. Critical appraisal was applied using the Mixed‐Methods Appraisal Tools and the Joanna Briggs Critical Appraisal checklist. Qualitative Research in Psychology, 3(2), 77–101, thematic analysis method was used to generate themes.ResultsSeven research articles and three national reports were included. Four themes—in‐depth nursing knowledge and improving prophylaxis adherence, cultural competency, and therapeutic nurse–patient relationships—were found.ConclusionWhile nursing knowledge and ways to improve injection adherence are essential, being culturally receptive and developing therapeutic relationships are equally important. Without strong and trusting relationships, it is difficult to deliver care required for prevention success.Implications to careWhen working with vulnerable populations it is important to be culturally receptive in all interactions with patients and their families.ImpactNew Zealand has high rates of rheumatic fever, especially among vulnerable populations such as Pacific Islanders and Māori. Nurses are often frontline primary care providers who, when skilled with the right tools, can help reduce the prevalence of this disease.Reporting methodThe Preferred Reporting Items for Systematic Reviews and Meta‐Analysis flow chart.Patient or public contributionNo Patient or Public Contribution was required for this research.