bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct magnetic separation of immune cells from whole blood using bacterial magnetic particles displaying protein G
Authors: Takahashi Masayuki , Yoshino Tomoko , Takeyama Haruko , Matsunaga Tadashi 
Published: February 2009

AbstractDirect separation of target cells from mixed population, such as peripheral blood, umbilical cord blood, and bone marrow, is an essential technique for various therapeutic or diagnosis applications. In this study, novel particles were fabricated, and direct magnetic separation of immune cells from whole blood using such particles was performed. The magnetotactic bacterium Magnetospirillum magneticum AMB‐1 synthesizes intracellular bacterial magnetic particles (BacMPs), and protein G was expressed on the surface of the BacMPs by gene fusion techniques with anchor proteins isolated from BacMP membrane. The BacMPs displaying protein G (protein G‐BacMPs) had high binding capabilities to a wide range of antibody types, and various versions of protein G‐BacMPs binding with different anti‐CD monoclonal antibodies were constructed. Consequently, direct magnetic separation of immune cells from whole blood using protein G‐BacMPs binding with anti‐CD monoclonal antibodies was demonstrated. B lymphocytes (CD19+ cells) or T lymphocytes (CD3+ cells), which represent less than 0.05% in whole blood cells, were successfully separated at a purity level of more than 96%. This level was superior to that from previous reports using other magnetic separation approaches. The results of this study demonstrate the utility of protein G‐BacMP and this particle may become a powerful tool for various therapeutic or diagnosis applications. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
Scalable culture and cryopreservation of human embryonic stem cells on microcarriers
Authors: Nie Ying , Bergendahl Veit , Hei Derek J. , Jones Jeffrey M. , Palecek Sean P. 
Published: February 2009

AbstractAs a result of their pluripotency and potential for unlimited self‐renewal, human embryonic stem cells (hESCs) hold tremendous promise in regenerative medicine. An essential prerequisite for the widespread application of hESCs is the establishment of effective and efficient protocols for large‐scale cell culture, storage, and distribution. At laboratory scales hESCs are cultured adherent to tissue culture plates; these culture techniques are labor‐intensive and do not scale to high cell numbers. In an effort to facilitate larger scale hESC cultivation, we investigated the feasibility of culturing hESCs adherent to microcarriers. We modified the surface of Cytodex 3 microcarriers with either Matrigel or mouse embryonic fibroblasts (MEFs). hESC colonies were effectively expanded in a pluripotent, undifferentiated state on both Matrigel‐coated microcarriers and microcarriers seeded with a MEF monolayer. While the hESC expansion rate on MEF‐microcarriers was less than that on MEF‐plates, the doubling time of hESCs on Matrigel‐microcarriers was indistinguishable from that of hESCs expanded on Matrigel‐coated tissue culture plates. Standard hESC cryopreservation methodologies are plagued by poor viability and high differentiation rates upon thawing. Here, we demonstrate that cryopreservation of hESCs adherent to microcarriers in cryovials provides a higher recovery of undifferentiated cells than cryopreservation of cells in suspension. Together, these results suggest that microcarrier‐based stabilization and culture may facilitate hESC expansion and storage for research and therapeutic applications. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
C. botulinum inactivation kinetics implemented in a computational model of a high‐pressure sterilization process
Authors: Juliano Pablo , Knoerzer Kai , Fryer Peter J. , Versteeg Cornelis 
Published: February 2009

AbstractHigh‐pressure, high‐temperature (HPHT) processing is effective for microbial spore inactivation using mild preheating, followed by rapid volumetric compression heating and cooling on pressure release, enabling much shorter processing times than conventional thermal processing for many food products. A computational thermal fluid dynamic (CTFD) model has been developed to model all processing steps, including the vertical pressure vessel, an internal polymeric carrier, and food packages in an axis‐symmetric geometry. Heat transfer and fluid dynamic equations were coupled to four selected kinetic models for the inactivation of C. botulinum; the traditional first‐order kinetic model, the Weibull model, an nth‐order model, and a combined discrete log‐linear nth‐order model. The models were solved to compare the resulting microbial inactivation distributions. The initial temperature of the system was set to 90°C and pressure was selected at 600 MPa, holding for 220 s, with a target temperature of 121°C. A representation of the extent of microbial inactivation throughout all processing steps was obtained for each microbial model. Comparison of the models showed that the conventional thermal processing kinetics (not accounting for pressure) required shorter holding times to achieve a 12D reduction of C. botulinum spores than the other models. The temperature distribution inside the vessel resulted in a more uniform inactivation distribution when using a Weibull or an nth‐order kinetics model than when using log‐linear kinetics. The CTFD platform could illustrate the inactivation extent and uniformity provided by the microbial models. The platform is expected to be useful to evaluate models fitted into new C. botulinum inactivation data at varying conditions of pressure and temperature, as an aid for regulatory filing of the technology as well as in process and equipment design. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
The meaning of style? Style reflexivity among Danish high school youths
Authors: Kjeldgaard Dannie 
Published: February 2009

Abstract

In much of the cultural theory literature on youth, style has been said to have started as an expression of subcultural class and transformed into individualized identity projects in neo‐tribal sociality. This article discusses young, Danish consumers' discourses on style and style practice. The key characteristic of their style practice is the reflexivity they bring to bear on their negotiation of global style expression in local identity projects. This style reflexivity manifests itself in two themes: style switching and style code reflexivity. The article shows that consumers' reflexivity of style consumption constitutes not only a ‘folk theory’ of consumption that reflects the academic and popular vernacular of style, but also postmodern consumer research scholarship's mode of expression about style. How individual consumers handle style in a reflexive manner through folk theories of the market is unexplored in previous research on the consumption of style.

Copyright © 2009 John Wiley & Sons, Ltd.
Sortase‐Mediated Ligation: A Gift from Gram‐Positive Bacteria to Protein Engineering
Authors: Tsukiji Shinya , Nagamune Teruyuki 
Published: February 2009

AbstractA new enzymatic protein ligation tool, sortase, has recently emerged from Gram‐positive bacteria. This article outlines the technique, sortase‐mediated ligation, and its applications in protein engineering, which include the introduction of unnatural molecules into proteins, protein immobilization, protein–protein conjugation, protein cyclization, as a self‐cleavable tag for protein expression, protein–PNA hybrids, neoglycoconjugates, and cell‐surface protein labeling, etc.magnified image
Batchwise and Continuous Organophilic Nanofiltration of Grubbs‐Type Olefin Metathesis Catalysts
Authors: Schoeps Dirk , Buhr Kristian , Dijkstra Marga , Ebert Katrin , Plenio Herbert 
Published: February 2009

AbstractRetained: An N‐heterocyclic carbene with eight cyclohexyl groups (see figure) provides increased electron density for a highly active olefin metathesis catalyst as well as sufficient steric bulk to allow the efficient separation of such a complex from the organic products in the solvent‐resistant nanofiltration.magnified imageA mass‐tagged N‐mesityl imidazolinium salt with four additional ‐CH2NCy2 substituents was synthesized, leading to a molecular mass of nearly 1100 g mol−1 in the corresponding carbene ligand. This mass‐tagged ligand was used to generate the respective Grubbs II and Grubbs–Hoveyda type complexes. The catalytic activity of the latter complex was tested in several olefin metathesis reactions and found to be slightly superior to that of the related N‐mesityl based complex. In batchwise solvent resistant nanofiltration experiments the ruthenium complex dissolved in toluene and following a metathesis reactions was efficiently retained (>99.8 %) by a single nanofiltration; the permeate contained less than 4 ppm of Ru. Equally efficient catalyst retention was observed in a membrane reactor utilized for the continuous synthesis of a RCM product.
ChemInform Abstract: Polar Domains and Charge‐Density Waves in the Acentric Cerium(III) Iron(II) Sulfide Ce22Fe21S54.
Authors: Mills Allison M. , Ruck Michael 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chromium‐Based Clinopyroxene‐Type Germanates NaCrGe2O6 and LiCrGe2O6 at 298 K.
Authors: Redhammer Guenther J. , Roth Georg , Amthauer Georg 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Crystal Structures of NiPdTe and Ni2PdSe2.
Authors: Bichler Daniel , Pocha Regina , Loehnert Catrin , Johrendt Dirk 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis, Characterization, and Sorption Properties of Amorphous Titanium Phosphate and Silica‐Modified Titanium Phosphates.
Authors: Maslova Marina V. , Rusanova Daniela , Naydenov Valeri , Antzutkin Oleg N. , Gerasimova Lidia G. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis, Crystal Structure, Infrared and Raman Spectra of Sr4Cu3(AsO4)2 (AsO3OH)4×3H2O and Ba2Cu4(AsO4)2 (AsO3OH)3.
Authors: Dordevic Tamara , Karanovic Ljiljana 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Separation of Diastereoisomeric 1‐Aryl‐3‐piperidino(morpholino)‐2‐phenylpropan‐1‐ols and Their Hydrochlorides.
Authors: Gevorgyan G. A. , Gasparyan N. K. , Agababyan A. G. , Panosyan G. A. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Functionalized Dialkyl Cyclobutane‐1,1‐dicarboxylates and Alkyl 5,6‐Dihydro‐4H‐pyran‐3‐carboxylates via Michael‐Induced Ring Closure of δ‐Chloro‐α,β‐Unsaturated Diesters and Ketoesters.
Authors: Mangelinckx Sven , Vermaut Bruno , Verhe Roland , De Kimpe Norbert 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Oxidation of Cyclic Sulfides Catalyzed by an Aluminum(salalen) Complex as the Catalyst.
Authors: Matsumoto Kazuhiro , Yamaguchi Tetsufumi , Katsuki Tsutomu 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Stereospecific Palladium‐Catalyzed Route to Monoalkyl Diazenes for Mild Allylic Reduction.
Authors: Movassaghi Mohammad , Ahmad Omar K. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Direct Synthesis of 2,3,5‐Trichloro‐4,4‐dimethoxy‐ (IV) and 2,5‐Dichloro‐3,4,4‐trimethoxycyclopent‐2‐en‐1‐ones (III) from Hexachlorocyclopentadiene and Some Aspects of Their Reactivity.
Authors: Ivanova N. A. , Kislitsina K. S. , Miftakhov M. S. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: One‐Pot Synthesis of Fused Tricyclic Heterocycles with Quaternary Carbon Stereocenter by Sequential Pauson—Khand Reaction and Formal [3 + 3] Cycloaddition.
Authors: Fan Liyan , Zhao Wanxiang , Jiang Weihua , Zhang Junliang 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 2‐Phospha[3]ferrocenophanes with Planar Chirality: Synthesis and Use in Enantioselective Organocatalytic [3 + 2] Cyclizations.
Authors: Voituriez Arnaud , Panossian Armen , Fleury‐Bregeot Nicolas , Retailleau Pascal , Marinetti Angela 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient Reagent for Aldehyde to Alkyne Homologation.
Authors: Taber Douglass F. , Bai Sha , Guo Peng‐fei 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A General and Environmentally Benign Catalytic Reduction of Nitriles to Primary Amines.
Authors: Enthaler Stephan , Addis Daniele , Junge Kathrin , Erre Giulia , Beller Matthias 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ruthenium Hydride Catalyzed Addition of Aldehydes to Dienes Leading to β,γ‐Unsaturated Ketones.
Authors: Omura Sohei , Fukuyama Takahide , Horiguchi Jiro , Murakami Yuji , Ryu Ilhyong 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Asymmetric Synthesis of the Docetaxel (Taxotere) Side Chain: Organocatalytic Highly Enantioselective Synthesis of Esterification‐Ready α‐Hydroxy‐β‐amino Acids.
Authors: Dziedzic Pawel , Vesely Jan , Cordova Armando 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cobalt‐Catalyzed Regioselective Synthesis of Indenamine from o‐Iodobenzaldimine and Alkyne: Intriguing Difference to the Nickel‐Catalyzed Reaction.
Authors: Liu Chuan‐Che , Korivi Rajendra Prasad , Cheng Chien‐Hong 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structural Aspects of Iodine‐Promoted One‐Pot Cyclization of σ‐Bis(methylthio)stilbenes to Thieno[3,2‐b]thiophene Derivatives: Synthetic Trials of Tetrathienoacenes from 1,2‐Bis(3‐methylthiothiophen‐2‐yl)ethenes.
Authors: Yamamoto Tatsuya , Miyazaki Eigo , Takimiya Kazuo 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Regioselectivity in the Reactions of N‐(Polychloroethylidene)sulfonamides with 1H‐Pyrrole and 1‐Methyl‐1H‐pyrrole.
Authors: Rozentsveig I. B. , Shainyan B. A. , Kondrashov E. V. , Rudyakova E. V. , Rozentsveig G. N. , Chernyshev K. A. , Levkovskaya G. G. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Highly Enantioselective Friedel—Crafts Reaction of 4,7‐Dihydroindoles with β,γ‐Unsaturated α‐Keto Esters by Chiral Broensted Acids.
Authors: Zeng Mi , Kang Qiang , He Qing‐Li , You Shu‐Li 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chemistry of Aminophenols. Part 11. Synthesis of 3‐Arylideneindolin‐2‐ones from 2‐Aminophenols by Ugi Four‐Component Reaction and Heck Carbocyclization.
Authors: Dai Wei‐Min , Shi Jianyu , Wu Jinlong 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Studies on Antidepressant and Anticonvulsant Activities of Some 3‐(2‐Thienyl)pyrazoline Derivatives
Authors: Ozdemir Zuhal , Kandilci H. Burak , Gumusel Bulent , Calis Unsal , Bilgin A. Altan 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of a New Class of 2‐Oxazolines and 2‐Thiazolines
Authors: Padmaja A. , Payani T. , Mahesh K. 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 2‐Nitrosobenzothiazoles: Useful Synthons for New Azobenzothiazole Dyes.
Authors: Faustino Helio , El‐Shishtawy Reda M. , Reis Lucinda V. , Santos Paulo F. , Almeida Paulo 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of New 1‐Substituted 4‐Perfluoroalkyl Tetrazol‐5‐ones
Authors: Mekni Nejib , Baklouti Ahmed 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 2‐Alkylidene‐1,3‐dithiane Monoxides as Activated Alkenes in Rhodium‐Catalyzed Addition Reaction of Arylboronic Acids.
Authors: Yoshida Suguru , Yorimitsu Hideki , Oshima Koichiro 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Preparation and Cope Rearrangement of 2‐CF3‐cis‐2,3‐Bis(alkenyl)oxiranes: A Facile Route to 2‐CF3‐Substituted Oxacycles.
Authors: Shimizu Masaki , Fujimoto Takuya , Liu Xinyu , Takeda Youhei , Hiyama Tamejiro 
Published: February 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.