bluuebunny/crossref_metadata_2025_split huggingface dataset:
Replicative senescence, telomere shortening and cell proliferation rate in Gaddi goat's skin fibroblast cell line
Authors: Gupta Neelam , Taneja Ritu , Pandey Alok , Mukesh Manishi , Singh Hari , Gupta S.C. 
Published: May 2007

AbstractWe assessed aging in continuous donor skin fibroblast cell line GGM5 up to the 25th passage by in vitro replicative senescence, telomere dynamics and chromosomal abnormalities. Cell proliferation rate increased from 0.84 ± 0.26 (primary cells) to 1.20 ± 0.17 (13–15 passage group) per day and reduced to 0.65 ± 0.14 in 22–25 passages. Cell proliferation rate was reduced by 45.7% after 87.62 CPDs. Cell viability reduced from 100% to 97.4% up to the 25th passages. Frequency of β gal+ cells increased in successive passages and days in culture. The correlation coefficient between frequency of β gal+ cells and growth rate was −0.50 to −0.61. Loss of mean TRF length was 13.8 nucleotides (passage 15) to 95.4 nucleotides per cell division in later passages. All cells showed Robertsonian translocation in 22–25 passaged cells. The SCNT pre‐implantation embryos production was highest (22.5%) in donor cells used from 10–15 passages as compared to early (≤5) and late (22–25 passages). Our findings supports that cell proliferation rates, β gal staining, mean TRF loss and karyological profile are useful marker for evaluation of competent nuclear donor.
Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation‐induced apoptosis of AHH‐1 human lymphoblastoid cells
Authors: Wang Zi‐Bing , Liu Yu‐Qing , Zhang Ying , Li Yan , An Xiao‐Xia , Xu Han , Guo Ying , Jin Wei , Jiang Zhu‐Jun , Cui Yu‐Fang 
Published: May 2007

AbstractThe aim of the study was to investigate the sensitivity of AHH‐1 human lymphoblastoid cells to radiation and its relevance to intracellular events, specifically alteration in cellular energy‐producing systems. AHH‐1 human lymphoblastoid cells were irradiated with 6 Gy of γ radiation, and then were collected at the indicated time points. Parallel studies were conducted to assess the effects of radiation on the cell proliferation and apoptotic index. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production were monitored. A marked decrease of cell viability was observed as early as 12 h postirradiation and fraction of apoptotic cells was highest at 24 h. Intracellular ROS generation measured with 2′,7′‐dichlorodihydrofluorescein diacetate (DCFH‐DA) appeared to be highest as early as 30 min postirradiation and resumed to normal level at 6 h. Unexpectedly, the fluorescence intensity of Rhodamine 123 for measuring MMP did not change during the first 3 h after radiation and exhibited an aberrant increase at 6 h. The results suggest that AHH‐1 cells are sensitive to radiation‐induced apoptosis and ROS generation is an early phase in the apoptosis process. Moreover, the results might cast doubts on those studies using Rhodamine 123 which hypothesized that the fall in MMP is one of the early events of apoptosis.
Complications of three sacrospinous ligament fixation techniques
Authors: Pollak J. , Takacs P. , Medina C. 
Published: May 2007

AbstractObjectives: To compare the complications of three techniques used to pass the suture through the sacrospinous ligament when performing sacrospinous ligament fixation (SSLF): (1) the standard needle driver with direct visualization; (2) the Deschamps ligature carrier by palpation; and (3) the Miya hook ligature carrier by palpation. Materials and methods: A retrospective chart review of 240 patients undergoing SSLF was performed. A standard needle driver with direct visualization was used for 46 patients; the Deschamps ligature carrier was used for 173, and the Miya hook for 21 patients. Intraoperative and postoperative complications were compared between the groups. Results: Twelve women (5%) had intraoperative and forty women (17%) had postoperative complications suspected directly from the suture placement. There was no statistically significant difference in the rate of total intraoperative complications among the three groups. The proportion of patients with postoperative complications that were felt to be directly related to the suture passage technique was significantly higher in the Deschamps group compared to the direct visualization group (18% vs 2%, P = 0.002). Conclusion; Passing the suture through the sacrospinous ligament under direct visualization may result in less intra and postoperative complications.
Episodes of late Holocene aridity recorded by stalagmites from Devil's icebox Cave, Central Missouri, USA
Authors: Denniston Rhawn F. , DuPree Michelle , Dorale Jeffrey A. , Asmerom Yemane , Polyak Victor J. , Carpenter Scott J. 
Published: May 2007

AbstractTwo stalagmites from Devil's Icebox Cave, central Missouri, display similar δ13C and δ18O values and trends during the late Holocene. Positive δ13C excursions at 3.5–2.6 ka and 1.2–0.9 ka are interpreted to reflect drier conditions. These elevated stalagmite δ13C values could have plausibly been driven by increasing C4 plant abundances over the cave or an increased contribution of bedrock carbon, both of which could reflect decreased effective moisture. A lack of corresponding oxygen isotopic anomalies during these intervals suggests that neither mean annual temperature nor the seasonality of precipitation changed concomitantly with dryness. Both of the δ13C excursions identified in our stalagmite record are roughly coincident with dry intervals from a number of sites located across the Great Plains.
Comparative Analysis of Splice Site Regions by Information Content
Authors: Rekha T. Shashi , Mitra Chanchal K. 
Published: May 2007

Abstract
We have applied concepts from information theory for a comparative analysis of donor (gt) and acceptor (ag) splice site regions in the genes of five different organisms by calculating their mutual information content (relative entropy) over a selected block of nucleotides. A similar pattern that the information content decreases as the block size increases was observed for both regions in all the organisms studied. This result suggests that the information required for splicing might be contained in the consensus of ~6–8 nt at both regions. We assume from our study that even though the nucleotides are showing some degrees of conservation in the flanking regions of the splice sites, certain level of variability is still tolerated, which leads the splicing process to occur normally even if the extent of base pairing is not fully satisfied. We also suggest that this variability can be compensated by recognizing different splice sites with different spliceosomal factors.
A Network Partition Algorithm for Mining Gene Functional Modules of Colon Cancer from DNA Microarray Data
Authors: Ruan Xiao-Gang , Wang Jin-Lian , Li Jian-Geng 
Published: May 2007

Abstract
Computational analysis is essential for transforming the masses of microarray data into a mechanistic understanding of cancer. Here we present a method for finding gene functional modules of cancer from microarray data and have applied it to colon cancer. First, a colon cancer gene network and a normal colon tissue gene network were constructed using correlations between the genes. Then the modules that tended to have a homogeneous functional composition were identified by splitting up the network. Analysis of both networks revealed that they are scale-free. Comparison of the gene functional modules for colon cancer and normal tissues showed that the modules’ functions changed with their structures.
KaKs_Calculator: Calculating Ka and Ks Through Model Selection and Model Averaging
Authors: Zhang Zhang , Li Jun , Zhao Xiao-Qian , Wang Jun , Wong Gane Ka-Shu , Yu Jun 
Published: May 2007

Abstract
KaKs_Calculator is a software package that calculates nonsynonymous (Ka) and synonymous (Ks) substitution rates through model selection and model averaging. Since existing methods for this estimation adopt their specific mutation (substitution) models that consider different evolutionary features, leading to diverse estimates, KaKs_Calculator implements a set of candidate models in a maximum likelihood framework and adopts the Akaike information criterion to measure fitness between models and data, aiming to include as many features as needed for accurately capturing evolutionary information in protein-coding sequences. In addition, several existing methods for calculating Ka and Ks are also incorporated into this software. KaKs_Calculator, including source codes, compiled executables, and documentation, is freely available for academic use at http://evolution.genomics.org.cn/software.htm.
Body fat content and feeding level interact strongly in the short- and medium-term regulation of plasma leptin during underfeeding and re-feeding in adult sheep
Authors: Delavaud Carole , Bocquier François , Baumont René , Chaillou Elodie , Ban-Tokuda Tomomi , Chilliard Yves 
Published: May 2007

Circulating leptin is regulated by food intake in the long, medium and short term; however, little is known about putative remnant effects of these successive regulations at any given time. To clarify this, two experiments were conducted in adult sheep, during which body condition parameters and plasma leptin were measured. During experiment 1, twenty ewes with normal body condition were either well fed (101 % of maintenance energy requirements (MER)) or underfed (41 % MER) for 166 d, then rapidly re-fed (at a mean of 208 % MER) for 3 d. Leptinaemia decreased after 14 d of underfeeding, remained depressed until day 166 and did not increase after 3 d re-feeding, whereas it was increased (+153 %;P < 0·05) by re-feeding the previously well-fed ewes. During experiment 2, twenty-four fat or lean ewes were either well fed (114 % MER) or underfed (52 % MER) for 94 d, and gradually re-fed for 2 d and maintained at a high feeding level (235 % MER) for 9 d. Underfeeding decreased leptinaemia in fat (from 4·19 to 2·63 ng/ml) but not lean ewes, and re-feeding increased leptinaemia after 5 d in lean previously well-fed (+123 %;P < 0·05) but not underfed ewes. In fat ewes, the impact of re-feeding was rapid (+144 %;P < 0·001 at 5 d) in previously well-fed ewes, whereas it was more gradual with a maximum at 11 d (+162 %;P < 0·01) in previously underfed ewes. In conclusion, leptinaemia is modulated by short-term energy intake level in interaction with long-term regulations involving nutritional history and body fatness, suggesting that a biological threshold of adiposity (about 20 %) is necessary to allow short- and medium-term leptin regulation.
Dietary patterns and their correlates among middle-aged and elderly Chinese men: a report from the Shanghai Men's Health Study
Authors: Cai Hui , Zheng Wei , Xiang Yong-Bing , Hong Xu Wang , Yang Gong , Li Honglan , Ou Shu Xiao 
Published: May 2007

Dietary patterns, which reflect the complexity of food preference, lifestyle and socio-economic status, may play a major role in health and longevity. Understanding dietary patterns and their correlates is important to the research of diet and health relationships. In the Shanghai Men's Health Study (SMHS) a total of 61 582 men aged 40–74 were recruited between 2002 and 2006. Their food intake over the previous year was collected using a validated FFQ. Study participants (75·6 %) reported little or no change in meat and vegetable intake in the 5 years prior to recruitment. Using the baseline data of the SMHS, we assessed dietary patterns, as well as their relationship with socio-demographic and lifestyle factors and with prevalence of some chronic diseases. Three major dietary patterns, fruit-, vegetable- and meat-based diets, were identified in our population. Consumption of the fruit diet appeared to be more common among men who were older and more physically active, had higher income, and lower waist-to-hip ratio (WHR), while this diet was less common among manual labourers. The meat- or vegetable-based diets were less common among elderly men and more common among men with higher WHR. Dietary patterns appeared to be associated with the presence of health conditions. In general, subjects with a chronic disease were more likely to have the vegetable-based diet and less likely to have the fruit- or meat-based diets. Future studies of diet and health in this population will need to carefully take into account these potential confounders.
Acquired immunodeficiency syndrome related Kaposi's sarcoma eroding the maxillary bone
Authors: Konstantinopoulos P A , Goldsztein H , Dezube B J , Pantanowitz L 
Published: May 2007

AbstractBackground:Oral involvement is a common manifestation of acquired immunodeficiency syndrome related Kaposi's sarcoma, and it occurs in approximately one-third of afflicted patients.Methods:We report an unusual case of Kaposi's sarcoma of the hard palate which invaded the underlying maxillary bone.Results:The patient underwent excisional biopsy and was subsequently treated with localised radiation and systemic chemotherapy including liposomal doxorubicin.Conclusions:The presentation, differential diagnosis, radiographic appearance and management of this serious complication are discussed. Our case highlights the need for a multidisciplinary approach, consisting of ENT, oncology and infectious disease consultation, to ensure appropriate management of maxillofacial, intraosseous, acquired immunodeficiency syndrome related Kaposi's sarcoma.
Mutational falsetto: intervention outcomes in 45 patients
Authors: Dagli M , Sati I , Acar A , Stone R E , Dursun G , Eryilmaz A 
Published: May 2007

AbstractObjective:The aim of this study was to evaluate the outcomes of therapeutic intervention in patients with mutational falsetto, by applying perceptual and acoustic analysis before and after voice therapy.Materials and methods:Forty-five consecutive patients with mutational falsetto were studied retrospectively. Acoustic analysis (i.e. fundamental frequency, jitter, shimmer, and formants one, two and three) was performed using the Multi-Dimensional Voice Program. Perceptual voice analyses were performed, including graded severity–roughness–breathiness–aesthenicity–strain assessment.Results:Subjects' fundamental frequency, voice formants one, two and three, jitter, and shimmer were greater before than after treatment. There were statistically significant differences between pre- and post-treatment average values for fundamental frequency, jitter and shimmer. There were also statistically significant differences between pre- and post-treatment average values for formants one and two. These results were maintained after six months of follow up, and there was no significant difference between results at three- and six-month follow up. According to perceptual evaluation, each subject's voice had altered from mutational falsetto to chest voice by completion of the intervention. Thus, all of the patients successfully lowered their modal speaking voice to an appropriate level.Conclusion:In the light of objective evaluations, and by applying the study treatment protocol, these results suggest that normal voice can be maintained after intervention, at six months' follow up.
Wave kinetic description of quantum pair plasmas
Authors: MENDONÇA J. T. , RIBEIRO J. E. , SHUKLA P. K. 
Published: May 2007

AbstractThe dispersion relation for a quantum pair plasma is derived, by using a wave kinetic description. A general form of the kinetic dispersion relation for electrostatic waves in a two-component quantum plasma is established. The particular case of an electron–positron pair plasma is considered in detail. Exact expressions for Landau damping are derived, and the quasi-classical limit is discussed.
Argument diagramming in logic, law and artificial intelligence
Authors: REED CHRIS , WALTON DOUGLAS , MACAGNO FABRIZIO 
Published: May 2007

AbstractIn this paper, we present a survey of the development of the technique of argument diagramming covering not only the fields in which it originated — informal logic, argumentation theory, evidence law and legal reasoning — but also more recent work in applying and developing it in computer science and artificial intelligence (AI). Beginning with a simple example of an everyday argument, we present an analysis of it visualized as an argument diagram constructed using a software tool. In the context of a brief history of the development of diagramming, it is then shown how argument diagrams have been used to analyse and work with argumentation in law, philosophy and AI.
Propositional theories are strongly equivalent to logic programs
Authors: CABALAR PEDRO , FERRARIS PAOLO 
Published: May 2007

AbstractThis paper presents a property of propositional theories under the answer sets semantics (called Equilibrium Logic for this general syntax): any theory can always be reexpressed as a strongly equivalent disjunctive logic program, possibly with negation in the head. We provide two different proofs for this result: one involving a syntactic transformation, and one that constructs a program starting from the countermodels of the theory in the intermediate logic of here-and-there.
Suzaku observation of the black hole transient 4U1630–472: discovery of absorption lines
Authors: Kubota Aya , Dotani Tadayasu , Cottam Jean , Kotani Taro , Done Chris , Ueda Yoshihiro , Fabian Andy C. , Yasuda Tomonori , Takahashi Hiromitsu , Fukazawa Yasushi , Yamaoka Kazutaka , Makishima Kazuo , Yamada Shinya , Kohmura Takayoshi , Angelini Lorella ,   the Suzaku team
Published: May 2007

AbstractWe present the results of six Suzaku observations of the recurrent black hole transient 4U 1630–472 during its decline from its most recent outburst in 2006. All observations show the typical high/soft state spectral shape in the 2–50 keV band, roughly described by an optically thick disk spectrum in the soft energy band plus a weak power-law tail.The disk temperature decreases from 1.4 keV to 1.2 keV as the flux decreases by a factor 2, consistent with a constant radius as expected for disk-dominated spectra. All the observations reveal significant absorption lines from highly ionized (H-like and He-like) iron Kα at 7.0 keV and 6.7 keV.The energies of these absorption lines suggest a blue shift with an outflow velocity of ∼1000 km s−1. The H–like iron Kα equivalent width remains approximately constant at ∼30 eV over all the observations, while that of the He–like Kα line increases from 7 eV to 20 eV. Thus the ionization state of the material decreases, as expected from the decline in flux.The data constrain the velocity dispersion of the absorber to 200–2000 km s−1, and the size of the plasma as ∼1010 cm assuming a source distance of 10 kpc.
Massive black holes: formation and evolution
Authors: Rees Martin J. , Volonteri Marta 
Published: May 2007

AbstractSupermassive black holes are nowadays believed to reside in most local galaxies. Observations have revealed us vast information on the population of local and distant black holes, but the detailed physical properties of these dark massive objects are still to be proven. Accretion of gas and black hole mergers play a fundamental role in determining the two parameters defining a black hole: mass and spin. We briefly review here the basic properties of the population of supermassive black holes, focusing on the still mysterious formation of the first massive black holes, and their evolution from early times to now.
Variable and polarized emission from SgrA*
Authors: Eckart A. , Schödel R. , Meyer L. , Straubmeier C. , Dovčiak M. , Karas V. , Morris M. R. , Baganoff F. K. 
Published: May 2007

AbstractThe super-massive black hole in the Galactic Center (Sagittarius A*) is one of the most exciting targets in the sky. At a distance of ∼ 8 kpc it is about one hundred times closer than the second nearest nucleus of a similar galaxy, M31, and therefore the closest galactic nucleus that we can study. Here we report on the modeling of polarized near-infrared flare emission from SgrA* using a model in which a hot spot is moving on a relativistic orbit around the massive black hole. We also summarize the results from simultaneous radio/near-infrared/X-ray measurements of flare emission.
Observational evidence for intermediate-mass black holes: ultra-luminous X-ray sources
Authors: Makishima Kazuo 
Published: May 2007

AbstractIncorporating early data from the Suzaku satellite launched in July 2005, properties of Ultra-Luminous compact X-ray sources (ULXs) were studied in close comparison with those of Galactic and Magellanic black-hole binaries. Based on an analogy between these two types of X-ray sources, ULXs showing power-law type spectra are considered to host Comptonized accretion disks, while those with multicolor-disk type spectra are interpreted to harbor “slim” disks. The analogy also suggests that ULXs are radiating near their Eddington limits, and hence their central black holes are significantly more massive than the ordinary stellar-mass black holes contained in Galactic and Magellanic black-hole binaries. In this sense, ULXs can be regarded as intermediate-mass black holes.
The optical counterpart of the ultraluminous X-ray source NGC6946 ULX-1
Authors: Abolmasov P. K. , Fabrika S. N. , Sholukhova O. N. 
Published: May 2007

AbstractWe present a study of a peculiar nebula MF16 associated with an Ultraluminous X-ray Source NGC6946 ULX-1. We use integral-field and long-slit spectral data obtained with the 6-m telescope (Russia). The nebula was for a long time considered powered by strong shocks enhancing both high-excitation and low-excitation lines. However, kinematical properties point to rather moderate expansion rates (VS ∼ 100÷200 km s−1). The total power of the emission-line source exceeds by one or two orders of magnitude the power observed expansion rate can provide, that points towards the existence of an additional source of excitation and ionization. Using CLOUDY96.01 photoionization code we derive the properties of the photoionizing source. Its total UV/EUV luminosity must be about 1040 erg/s.
Recipes for ULX formation: necessary ingredients and garnishments
Authors: Soria Roberto 
Published: May 2007

AbstractWe summarize the main observational features that seem to recur more frequently in the ULX population. We speculate that low metal abundance, and clustered star formation triggered by molecular cloud collisions are two fundamental physical requirements for ULX formation. In this scenario, most ULXs are formed from recent stellar processes, have black hole (BH) masses <100 M⊙, and do not require merger processes in super-star-clusters.
Explosion of very massive stars and the origin of intermediate mass black holes
Authors: Tsuruta Sachiko , Ohkubo Takuya , Umeda Hideyuki , Maeda Keiichi , Nomoto Ken'ichi , Suzuki Tomoharu , Rees Martin J. 
Published: May 2007

AbstractWe calculate evolution, collapse, explosion, and nucleosynthesis of Population III very massive stars with 500 M⊙ and 1000 M⊙. It was found that both 500 M⊙ and 1000 M⊙ models enter the region of pair-instability but continue to undergo core collapse to black holes. For moderately aspherical explosions, the patterns of nucleosynthesis match the observational data of intergalactic and intercluster medium and hot gases in M82, better than models involving hypernovae and pair instability supernovae.Our results suggest that explosions of Population III core-collapse very massive stars contribute significantly to the chemical evolution of gases in clusters of galaxies. The final black hole masses are about 500 M⊙ for our most massive 1000 M⊙ models. This result may support the view that Population III very massive stars are responsible for the origin of intermediate mass black holes which were recently reported to be discovered.
The gravitational redshift in the broad line region of the active galactic nucleus Mrk 110
Authors: Gavrilović N. , Popović L.Č , Kollatschny W. 
Published: May 2007

AbstractWe used the long term spectroscopic observations of Mrk 110 (Hα and Hβ lines) to investigate the gravitational field influence on spectral line profiles. We found that effects of gravitational field can be measured and that the lines are more intense where the emission is originating close to the central black hole of Mrk 110.