bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forecasting migraine attacks by managing daily lifestyle: a systematic review as a basis to develop predictive algorithms
Authors: Quartetti Umberto , Brighina Filippo , Gambino Giuditta , Frinchi Monica , Bellafiore Marianna , Tabacchi Garden , Vasto Sonya , Accardi Giulia , Amato Antonella , Giardina Marta , Mazzucco Walter , Boffetta Paolo , Giglia Giuseppe , Di Liberto Valentina 
Published: February 2025

Abstract
Recent studies attempting to develop forecasting models for new migraine attack onsets, overviewing triggers and protectors, are encouraging but necessitate further improvements to produce forecasting models with high predictive accuracy. This updated review of available data holds the potential to enhance the precision of predicting a migraine attack. This study aims to evaluate how lifestyle factors affect migraine frequency in adults with episodic migraine, to contribute to the development of an effective migraine forecasting model. A comprehensive search of databases, including PubMed, ScienceDirect, Google Scholar, and Scopus, was conducted considering studies published from 2018 to December 2023, following the PRISMA guidelines. Critical evaluation was conducted using the Joanna Briggs Institute's appraisal tools. The lifestyle modifications examined in this review included dietary habits, physical activity, sleep, and stress management. Of the 36 studies analysed, which predominantly exhibited low to moderate bias, 18 investigated dietary habits, 7 explored physical activity, 11 assessed stress management, and 5 investigated sleep patterns. The evidence from these 36 studies advocates for the implementation of lifestyle modifications in migraine management. Furthermore, these outcomes carry valuable implications from the standpoint of migraine forecasting models. The most consistent results were observed in relation to specific diets, dietary supplements, and physical activity. Although trends were noted in stress management and sleep, further research is required to elucidate their influence on migraine frequency and their integration into a migraine forecasting model. This study is registered on PROSPERO (ID CRD42024511300).
Reduced peri-pubertal bone density accrual among Zimbabwean males living with HIV: a longitudinal study
Authors: Jeena Lisha , Ferrand Rashida A. , Simms Victoria , Kahari Cynthia , Bandason Tsitsi , Rukuni Ruramayi , Rehman Andrea M. , Rowland-Jones Sarah , Hsieh Anthony Y.Y. , Gregson Celia L. 
Published: February 2025

Objective:
To investigate bone density accrual over 1 year among peripubertal children living with HIV (CWH) compared to children without infection (CWOH); and risk factors associated with bone density accrual among CWH.

Design:
A prospective cohort study in urban Zimbabwe.

Methods:
CWH on antiretroviral therapy aged 8–16 years, and CWOH, frequency-matched by age were recruited in Zimbabwe. Z-scores for height-adjusted total-body less-head bone mineral content for lean mass (TBLH-BMCLBM) and size-adjusted lumbar spine bone mineral apparent density (LS-BMAD) were calculated from dual X-ray absorptiometry (DXA) scan measurements. Linear regression compared bone density accrual by HIV status.

Results:
Of 609 participants, 492 (80.7%) completed a follow-up visit (50.2% male, 49.6% CWH). Mean baseline age was 12.5 years. More female CWH than CWOH were in Tanner stages I/II at baseline. Bone density accrual (Δ) adjusted for age, Tanner stage and baseline DXA Z-score was less in male CWH than male CWOH {adjusted mean (95% confidence interval (CI)] ΔLS-BMAD Z-score −0.14 (−0.25 to −0.02) vs. 0.01 (−0.09 to 0.12), P = 0.020, and ΔTBLH-BMCLBM
Z-score −0.19 (−0.33 to −0.04) vs. 0.07 (−0.07 to 0.20), P = 0.015}, but similar in females with and without HIV [ΔLS-BMAD Z-score 0.05 (−0.07 to 0.17) vs. −0.01 (−0.09 to 0.07), P = 0.416, and ΔTBLH-BMCLBM
Z-score 0.08 (−0.07 to 0.22) vs. −0.03 (−0.12 to 0.07), P = 0.295]. Viral load greater than 1000 copies/ml and tenofovir disoproxil fumarate use were associated with less gain in LS-BMAD Z-score among males, whereas Tanner stage IV and V were associated with greater gains in LS-BMAD and TBLH-BMCLBM
Z-scores among CWH.

Conclusion:
Among males only, CWH had impaired bone accrual, associated with high viral load and tenofovir use. Bone density gains were greater in later puberty among CWH suggesting potential to correct deficits.
Physical, psychosocial and sexual health among people aged 50 years and above with and without HIV: a nationwide study
Authors: Scofield Ditte , Frisch Morten , Andersson Mikael , Storgaard Merete , Pedersen Gitte , Johansen Isik S. , Katzenstein Terese L. , Graugaard Christian , Omland Lars H. , Weis Nina , Moseholm Ellen 
Published: February 2025

Objective:
To investigate physical, psychosocial and sexual health outcomes in ≥50 years-old people with and without HIV.

Design:
Cross-sectional study in Denmark comparing health outcomes by HIV status.

Methods:
Data were drawn from the SHARE study, a nationwide survey conducted between 2021 and 2022, examining psychosocial, sexual and reproductive health in people with HIV (PWH), and compared to data from people without HIV (PWOH) in the nationally representative Project SEXUS cohort study. Health outcomes were assessed using validated patient-reported outcome measures, with sex-stratified logistic regression models adjusting for sociodemographic and other confounding factors.

Results:
The study included 322 men and 67 women with HIV and 15 548 men and 13 926 women without HIV. PWH were more likely to report being single and having non-Danish parentage. HIV was associated with higher odds of liver disease in both men and women, cardiovascular and lung diseases in men, and osteoporosis and hypertension in women. Compared with PWOH, men with HIV more often reported depressive symptoms or a history of treatment for anxiety, while women with HIV more often reported loneliness, suicidal thoughts and domestic abuse. Sexually, PWH more often reported reduced sexual desire and activity, men with HIV more often reported erectile dysfunction or orgasmic dysfunction and women with HIV more often reported lubrication dysfunction or genital pain dysfunction.

Conclusions:
PWH aged ≥50 years in Denmark face significantly more physical, psychosocial and sexual health challenges than PWOH. Clinical attention to these disparities is crucial for improving the overall health of ageing PWH.
Cerebrospinal fluid biomarkers of inflammation and immune activation associated with neurocognitive impairment among U.S. Latinos living with HIV
Authors: Diaz Monica M. , Kamalyan Lily , Al-rousan Tala , Breton Jordana , Franklin Donald R. , Umlauf Anya , Ellis Ronald J. , Cherner Mariana , Iudicello Jennifer , Heaton Robert K. , Letendre Scott L. , Marquine María J. 
Published: February 2025

Objective:
Examine the association between markers of inflammation in the cerebrospinal fluid (CSF) and neurocognitive impairment (NCI) among diverse persons with HIV (PWH).

Background:
Latino PWH are at higher risk for NCI than non-Latino White PWH (NLW). Evidence of inflammation in cerebrospinal fluid (CSF) can be higher among racial and ethnic minority PWH and has been linked to NCI.

Methods:
We performed a retrospective cross-sectional analysis of 363 PWH who identified as Latinos or NLW. Neurocognitive performance was measured by a comprehensive battery. A focused panel of biomarkers [interleukin-6 (IL-6), soluble CD14 (sCD14), interferon-γ-inducible protein-10 (IP-10), neurofilament light chain (NFL)] was measured in CSF by immunoassay. Covariates included demographic, HIV disease, medical, psychiatric, and substance use characteristics.

Results:
The cohort consisted of 126 Latinos and 237 NLW (age: M = 42.5, SD = 11.0, 88% male, 51.5% AIDS history; 64% on antiretroviral therapy). Latinos had significantly higher NFL levels than NLW (P < 0.0001, adjusted Cohen's d 1.15), but not among virally-suppressed PWH. In the entire cohort, higher sCD14 was associated with NCI (adjusted odds ratio (aOR) = 2.6, confidence interval (CI) = 1.1–6.5] after adjusting for statistically significant covariates.

Conclusions:
We did not identify a relationship between ethnicity, inflammation and NCI in this cohort. Future studies might examine sociocultural factors leading to increased inflammation in the CSF in diverse PWH.
Remote Site Delayed Extradural Hematoma Secondary to Decompressive Craniectomy for Traumatic Brain Injury: Incidence, Predictors, Characteristics, and Outcomes
Authors: Shen Jun , An Qian , Cao Jun , Fang Jingcheng , Xia Dayong , Jiang Xiaochun , Zhang Shaolin 
Published: February 2025

Objective:
Remote site delayed extradural hematoma (RDEDH) is an uncommon but serious complication that can occur after decompressive craniectomy (DC) in patients with traumatic brain injury (TBI). This study aims to clarify the incidence, predictors, clinical and radiological characteristics, and outcomes of RDEDH to improve the authors' understanding and management of this complication.

Methods:
This study identified 36 patients with TBI who developed RDEDH after DC. The incidence, predictors, clinical and radiological characteristics, and outcomes were analyzed retrospectively. Continuous variables were compared using the student t test, whereas categorical variables were assessed using the χ2 test. Variables with a P value of <0.05 in univariable analysis were included in the multivariable logistic regression analysis.

Results:
The incidence of RDEDH after DC was 14.6%. Preoperative pupillary dilation [bilaterally; odds ratio (OR): 8.797; 95% CI: 1.969–39.297; P = 0.004], intraoperative acute brain swelling (OR: 33.696; 95% CI: 5.073–227.005; P < 0.001), and pupillary abnormalities after DC (OR: 8.141; 95% CI: 2.117–31.307; P = 0.002) were identified as independent risk factors for the development of RDEDH after DC. The average length of hospitalization in the RDEDH group was significantly longer than that in the non-RDEDH group (P = 0.018). The Glasgow Outcome Scale score at 6 months in the RDEDH group was significantly lower than that in the non-RDEDH group (P = 0.01). The incidence of poor prognosis in the RDEDH group (80.6%) was significantly higher than that in the non-RDEDH group (59.8%; P = 0.017).

Conclusion:
Preoperative bilateral pupillary dilation, intraoperative brain swelling, and pupillary abnormalities after surgery were independent risk factors for the development of RDEDH after DC in patients with TBI. The occurrence of RDEDH in patients with TBI after DC did not significantly increase mortality, it was associated with a prolonged length of stay and an increased incidence of poor prognosis.
Open Globe Injury After Penetrating Keratoplasty: Etiology and Prognostic Factors
Authors: Özer Ömer , Dursun Özer 
Published: February 2025

Background:
This study aimed to evaluate the etiologic causes and prognostic factors affecting the final outcome of patients with open globe injury after penetrating keratoplasty.

Methods:
This study included patients between November 2018 and June 2024. Age, sex, intraocular pressure (mm Hg), visual acuity (logMAR), indication for keratoplasty, time since keratoplasty, cause, location, and length of injury were noted. All patients were followed up for at least 3 months after surgery.

Results:
There was a moderate positive correlation between visual acuity in the first month and the third month after trauma repair (r=0.562, P=0.036). Final visual acuity was 1.0 logMAR or worse in high injury length patients (6 clocks or more). Patients with vitreous loss had a final visual acuity of 0.9 logMAR or worse. Of the 5 patients who underwent PK for keratoconus, 4 (80%) had visual acuity of 1.2 logMAR or worse at the third month after trauma.

Conclusion:
In conclusion, final visual acuity is poor in patients who underwent PK due to high injury length, vitreous loss, and keratoconus. In addition, final visual acuity is associated with visual acuity in the early postoperative period. Patients undergoing PK should be informed about globe injuries, risks of visual loss, and protective precautions. Patients should also be enlightened about wound healing and encouraged to prevent trauma.
Mitochondrial Transplantation
Authors: Mukkala Avinash Naraiah , David Bruna Araujo , Ailenberg Menachem , Liang Jady , Vaswani Chirag Manoj , Karakas Danielle , Goldfarb Rachel , Barbour William , Gasner Avishai , Wu Ruoxian Scarlet , Petrut Raluca , Jerkic Mirjana , Andreazza Ana C. , dos Santos Claudia , Ni Heyu , Zhang Haibo , Kapus Andras , Kubes Paul , Rotstein Ori David 
Published: February 2025

Objective:
To investigate the hepatoprotective effects of mitochondrial transplantation in a murine liver ischemia/reperfusion (I/R) model.

Summary Background Data:
Sequential liver ischemia followed by reperfusion (I/R) is a pathophysiological process underlying hepatocellular injury in a number of clinical contexts, such as hemorrhagic shock/resuscitation, major elective liver surgery and organ transplantation. A unifying pathogenic consequence of I/R is mitochondrial dysfunction. Restoration of mitochondria via transplantation (MTx) has emerged as potential therapeutic in I/R. However, its role in liver I/R and its mechanisms of action remain poorly defined.

Methods:
We investigated the hepatoprotective effects of MTx in an in vivo mouse model of liver I/R and used in vivo imaging and various knockout and transgenic mouse models to determine the mechanism of protection.

Results:
We found that I/R-induced hepatocellular injury was prevented by MTx, as measured by plasma ALT, AST and liver histology. Additionally, I/R-induced pro-inflammatory cytokine release (IL-6, TNFα) was dampened by MTx, and anti-inflammatory IL-10 was enhanced. Moreover, MTx lowered neutrophil infiltration into both the liver sinusoids and lung BALF, suggesting a local and distant reduction in inflammation. Using in vivo intravital imaging, we found that I/R-subjected Kupffer cells (KCs), rapidly sequestered transplanted mitochondria, and acidified mitochondria within lysosomal compartments. To specifically interrogate the role of KCs, we depleted KCs using the diphtheria toxin-inducible Clec4f/iDTR transgenic mouse, then induced I/R, and discovered that KCs are necessary for the beneficial effects of MTx. Finally, we induced I/R in complement receptor of the immunoglobulin superfamily (CRIg) knockout mice and found that CRIg was required for mitochondria capture by KCs and mitochondrial-mediated hepatoprotection.

Conclusions:
In this study, we demonstrated that CRIg-dependent capture of mitochondria by I/R-subjected Kupffer cells is a hepatoprotective mechanism in vivo. These data progress knowledge on the mechanisms of MTx and opens new avenues for clinical translation.
Phylogeography and diversification of thePieris napispecies group in the Western Palaearctic
Authors: Sala-Garcia Javier , Vives-Ingla Maria , Tonzo Vanina , Dincă Vlad , Vila Roger , Carnicer Jofre 
Published: February 2025

AbstractThe butterflies of thePieris napicomplex, encompassing diverse taxa across Europe, Asia and North Africa, represent an emergent model system for studying climatic adaptation. In this study, we use genomic data for this group to address phylogenetic relationships, population structure and ecological differentiation in the Western Palearctic. Our results reveal two main groups: thenapiclade and thebryoniae-balcanaclade. We clarify the status and show that the distribution of the debated speciesP. balcanaextends to south Romania and the Peloponnese. This species is shown to be sister toP. bryoniae, which indicates that melanism is an evolutionarily labile trait linked to climatic adaptation. TheP. napiclade is divided into three components, each predominating in a different south European peninsula, and the borealP. n. adalwinda, which is notably differentiated. The endangered Moroccan taxonsegonzaciproduced conflicting results, probably due to low-quality DNA. Admixture analyses suggest substantial gene flow between taxa in contact areas. Redundancy analyses identify summer temperature and precipitation as key drivers of adaptive genetic variation within the group. This work provides a robust evolutionary framework for future ecological studies of thePieris napicomplex, to forecast eco-evolutionary responses and to address conservation priorities in a rapidly changing climate.
Hepatic Arterial Flow-Induced Portal Tract Fibrosis in Portal Hypertension: The Role of VCAM-1 and Osteopontin-Expressing Macrophages
Authors: Ma Ruixue , Gong Li , Dong Chao , Utsumi Teruo , Qi Jiewen , Zhuang Zhen W. , Zhang Xuchen , Yang Yilin , McConnell Matthew J. , Huang Hui-Chun , Iwakiri Yasuko 
Published: February 2025

ABSTRACTBackgroundThe liver undergoes significant hemodynamic changes during surgery, transplantation, or cirrhosis with portal hypertension(PH). The hepatic artery buffer response(HABR), which compensates for reduced portal venous flow by increasing hepatic artery(HA) flow, is hypothesized to induce pathological portal tract remodeling. This study investigates the molecular mechanisms underlying this process.MethodsPH was induced in Sprague-Dawley rats via partial portal vein ligation(PPVL). Structural evaluation(microCT), immune cell profiling, hemodynamic measurements, and transcriptomic analysis in macrophages(Mϕ) from sham or PPVL rats were conducted.ResultsMicroCT revealed decreased portal vein flow and increased HA flow correlated with portal pressure(r=0.799, p<0.01). A 2-fold increase in portal tract fibrosis(p<0.001) was observed with increased α-SMA+ myofibroblasts in PPVL rats. CD68+ Mϕ peaked at 10 days post-PPVL, and their depletion significantly reduced fibrosis(p<0.001), indicating critical roles of Mϕ in portal tract remodeling. VCAM-1 was elevated in HA endothelium and portal fibroblasts (PFs); VCAM-1 neutralization reduced collagen accumulation(p<0.05), CD68+ Mϕ(46.3%, p<0.01), and CD3+ T cells(18%, p<0.05). Mϕ-conditioned medium increased VCAM-1 in PFs(8-fold, p<0.001) and enhanced PF migration, while VCAM-1 knockdown reduced this effect (p<0.01). Single-cell RNA sequencing data(GSE171904) and RNA-FISH revealed increased interactions between osteopontin (Spp1)+ Mϕ and PFs, with Spp1+ Mϕ driving fibrosis. Spp1 knockdown in Mϕ co-culture reduced PF fibrogenic markers, while recombinant Spp1 upregulated Col1a1, Fn1, and Acta2 expression in PFs.ConclusionIncreased VCAM-1 in arterial endothelial cells and PFs facilitates the recruitment of Spp1+ Mϕ, which drive HA flow-mediated vascular remodeling and portal tract fibrosis. These findings highlight arterial flow-induced fibrosis as a key mechanism in PH, potentially contributing to disease progression and decompensation.SynopsisLiver hemodynamic changes in portal hypertension drive extracellular matrix accumulation and portal tract remodeling via Spp1+ macrophages. This study highlights how altered blood flow induces fibrosis, and its potential role in decompensation, and identifies therapeutic targets for advanced liver disease.
A Temporal and Spatial Atlas of Adaptive Immune Responses in the Lymph Node Following Viral Infection
Authors: Jiang Shaowen , Mantri Madhav , Maymi Viviana , Leddon Scott A. , Schweitzer Peter , Bhandari Subash , Holdener Chase , Ntekas Ioannis , Vollmers Christopher , Flyak Andrew I. , Fowell Deborah J. , Rudd Brian D. , De Vlaminck Iwijn 
Published: February 2025

ABSTRACTThe spatial organization of adaptive immune cells within lymph nodes is critical for understanding immune responses during infection and disease. Here, we introduce AIR-SPACE, an integrative approach that combines high-resolution spatial transcriptomics with paired, high-fidelity long-read sequencing of T and B cell receptors. This method enables the simultaneous analysis of cellular transcriptomes and adaptive immune receptor (AIR) repertoires within their native spatial context. We applied AIR-SPACE to mouse popliteal lymph nodes at five distinct time points after Vaccinia virus footpad infection and constructed a comprehensive map of the developing adaptive immune response. Our analysis revealed heterogeneous activation niches, characterized by Interferon-gamma (IFN-γ) production, during the early stages of infection. At later stages, we delineated sub-anatomical structures within the germinal center (GC) and observed evidence that antibody-producing plasma cells differentiate and exit the GC through the dark zone. Furthermore, by combining clonotype data with spatial lineage tracing, we demonstrate that B cell clones are shared among multiple GCs within the same lymph node, reinforcing the concept of a dynamic, interconnected network of GCs. Overall, our study demonstrates how AIR-SPACE can be used to gain insight into the spatial dynamics of infection responses within lymphoid organs.
Heterochromatin fidelity is a therapeutic vulnerability in lymphoma and other human cancers
Authors: Najia Mohamad Ali , Jha Deepak K. , Zhang Cheng , Laurent Benoit , Kubaczka Caroline , Markel Arianna , Li Christopher , Morris Vivian , Tompkins Allison , Hensch Luca , Qin Yue , Chapuy Bjoern , Huang Yu-Chung , Morse Michael , Marunde Matthew R. , Vaidya Anup , Gillespie Zachary B. , Howard Sarah A. , North Trista E. , Dominguez Daniel , Keogh Michael-Christopher , Schlaeger Thorsten M. , Shi Yang , Li Hu , Shipp Margaret M. , Blainey Paul C. , Daley George Q. 
Published: February 2025

ABSTRACTGenes involved in the regulation of chromatin structure are frequently disrupted in cancer, contributing to an aberrant transcriptome and phenotypic plasticity. Yet, therapeutics targeting mutant forms of chromatin-modifying enzymes have yielded only modest clinical utility, underscoring the difficulty of targeting the epigenomic underpinnings of aberrant gene regulatory networks. Here, we sought to identify novel epigenetic vulnerabilities in diffuse large B-cell lymphoma (DLBCL). Through phenotypic screens and biochemical analysis, we demonstrated that inhibition of the H3K9 demethylases KDM4A and KDM4C elicits potent, subtype-agnostic cytotoxicity by antagonizing transcriptional networks associated with B-cell identity and epigenetically rewiring heterochromatin. KDM4 demethylases associated with the KRAB zinc finger ZNF587, and their enzymatic inhibition led to DNA replication stress and DNA damage-induced cGAS-STING activation. Broad surveys of transcriptional data from patients also revealed KDM4 family dysregulation in several other cancer types. To explore this potential therapeutic avenue, we performed high-throughput small molecule screens with H3K9me3 nucleosome substrates and identified novel KDM4 demethylase inhibitors. AI-guided protein-ligand binding predictions suggested diverse modes of action for various small molecule hits. Our findings underscore the relevance of targeting fundamental transcriptional and epigenetic mechanisms for anti-cancer therapy.HIGHLIGHTSPhenotypic screens identified JIB-04 as a potent anti-cancer agent for multiple subtypes of diffuse large B-cell lymphomaJIB-04 binds and inhibits KDM4 demethylases resulting in epigenomic rewiring of heterochromatinKDM4 demethylases cooperate with KRAB zinc fingers to limit DNA replication stress, and KDM4 inhibition instigates DNA-damage and cGAS-STING activation in several human cancersHigh-throughput small molecule screens with semi-synthetic nucleosome substrates and AI-guided molecular docking simulations identify novel KDM4 inhibitors