bluuebunny/crossref_metadata_2025_split huggingface dataset:
Id‐1 promotes proliferation of p53‐deficient esophageal cancer cells
Authors: Hui Cheuk Man , Cheung Pak Yan , Ling Ming Tat , Tsao Sai Wah , Wang Xianghong , Wong Yong Chuan , Cheung Annie L.M. 
Published: February 2006

AbstractThe helix‐loop‐helix protein inhibitor of differentiation and DNA binding (Id‐1) is known to promote cellular proliferation in several types of human cancer. Although it has been reported that Id‐1 is over‐expressed in esophageal squamous cell carcinoma (ESCC), its function and signaling pathways in esophageal cancer are unknown. In our study, we investigated the direct effects of Id‐1 on esophageal cancer cell growth by transfecting an Id‐1 expression vector into an ESCC cell line (HKESC‐3), which showed serum‐dependent Id‐1 expression. Ectopic Id‐1 expression resulted in increased serum‐independent cell growth and G1‐S phase transition, as well as up‐regulation of mouse double minute 2 (MDM2) and down‐regulation of p21Waf1/Cip1 protein expressions in the transfectant clones in a p53‐independent manner. However, overexpression of Id‐1 had no effect on the pRB, CDK4 and p16INK4A expressions. Stable transfection of Id‐1 antisense expression vector to inhibit the expression of endogenous Id‐1 in another ESCC cell line (HKESC‐1) reversed the effects on MDM2 and p21Waf1/Cip1. In addition, Id‐1 expression protected ESCC cells from Tumor Necrosis Factor (TNF)‐α‐induced apoptosis by up‐regulating and activating Bcl‐2. In conclusion, our study provides evidence for the first time that Id‐1 plays a role in both proliferation and survival of esophageal cancer cells. Our findings also suggest that unlike prostate, hepatocellular and nasopharyngeal carcinomas in which Id‐1 induces cell proliferation through inactivation of p16INK4A/RB pathway, the increased cell proliferation observed in ESCC cells may be mediated through a different mechanism. © 2006 Wiley‐Liss, Inc.
The adhesion molecule L1 (CD171) promotes melanoma progression
Authors: Meier Friedegund , Busch Silke , Gast Daniela , Göppert Anne , Altevogt Peter , Maczey Evelyn , Riedle Svenja , Garbe Claus , Schittek Birgit 
Published: February 2006

AbstractThe adhesion molecule L1 is expressed in primary melanomas and cutaneous metastases in contrast to melanocytic nevi and melanocytes, and is significantly associated with metastatic spread. Recent studies have demonstrated that in carcinomas L1 expression is associated with sustained activation of the extracellular signal‐regulated kinase (ERK) pathway and upregulation of ERK‐dependent, motility‐ and invasion‐associated gene products including αvβ3 integrin. The objective of this study was to further investigate the role of the adhesion molecule L1 in melanoma progression, and to evaluate whether targeting the L1 adhesion molecule would have therapeutic effects against invasive melanoma growth. Using human melanoma cells from different stages of progression in monolayer and organotypic human skin culture mimicking the pathophysiological environment of cutaneous melanoma, we found that (1) L1 expression mostly correlates with melanoma progression and αvβ3 integrin expression, (2) overexpression of L1 in early radial growth phase melanoma cells promotes conversion from radial to vertical growth phase melanoma without upregulation of αvβ3 integrin expression, and (3) suppression of L1 function significantly reduces migration and invasion of melanoma cells, but does not completely block invasive melanoma growth. Altogether, L1 plays a critical role in melanoma invasion and progression and offers therapeutic potential in combination with conventional anticancer agents. © 2006 Wiley‐Liss, Inc.
Helicobacter pylori IgA and IgG antibodies, serum pepsinogen I and the risk of gastric cancer: Changes in the risk with extended follow‐up period
Authors: Knekt Paul , Teppo Lyly , Aromaa Arpo , Rissanen Harri , Kosunen Timo U. 
Published: February 2006

AbstractThe prediction of Helicobacter pylori antibodies immunoglobulin A (IgA) and immunoglobulin G (IgG) and serum pepsinogen I (PG I) on gastric cancer occurrence was studied in a nested case‐control study, based on 225 incident cancer cases and 435 matched controls from a Finnish cohort followed from 1966–1991. The odds ratio of noncardia gastric cancer between infected and noninfected persons was 3.12 (95% confidence interval (CI) = 1.97–4.95) for elevated IgA and 2.88 (CI: 1.63–5.07) for elevated IgG antibodies. The odds ratio between low and high PG I was 2.24 (CI: 1.43–3.49). The strength of association was significant for IgA antibodies during the total follow‐up, but for IgG antibodies this was only true for follow‐up periods of 15 years or more. IgA antibodies were significantly associated with all registered histological subtypes apart from intestinal type adenocarcinoma. The highest gastric cancer risk was found among individuals with simultaneously elevated IgA and IgG antibodies and low PG I with an odds ratio of 10.9 (CI: 4.31–27.7) in comparison with those who were negative for both antibodies and had normal PG I. Elevated IgA and IgG antibodies and low PG I were not associated with cancers of the gastric cardia. The findings support the hypothesis that H. pylori infection is a cause of noncardia gastric cancer. Although elevated H. pylori IgA and IgG antibodies and low PG I independently could predict the occurrence of noncardia gastric cancer, their power to do so varied with the stage and length of the follow‐up period and it increased when they were applied in combination. © 2006 Wiley‐Liss, Inc.
Breast cancer in Belarus and Ukraine after the Chernobyl accident
Authors: Pukkala Eero , Kesminiene Ausra , Poliakov Semion , Ryzhov Anton , Drozdovitch Vladimir , Kovgan Lina , Kyyrönen Pentti , Malakhova Irina V , Gulak Liudmila , Cardis Elisabeth 
Published: February 2006

AbstractAn increase in breast cancer incidence has been reported in areas of Belarus and Ukraine contaminated by the Chernobyl accident and has become an issue of public concern. The authors carried out an ecological epidemiological study to describe the spatial and temporal trends in breast cancer incidence in the most contaminated regions of Belarus and Ukraine, and to evaluate whether increases seen since 1986 correlate to radiation exposure from the Chernobyl accident. The authors investigated the trends through age‐cohort‐period‐region analyses of district‐specific incidence rates of breast cancer for Gomel and Mogilev regions of Belarus and Chernigiv, Kyiv and Zhytomir regions of Ukraine. Dose‐response analyses were based on Poisson regression, using average district‐specific whole body doses accumulated since the accident from external exposure and ingestion of long‐lived radionuclides. The study demonstrated increases in breast cancer incidence in all areas following the Chernobyl accident, reflecting improvements in cancer diagnosis and registration. In addition, a significant 2‐fold increase in risk was observed, during the period 1997–2001, in the most contaminated districts (average cumulative dose of 40.0 mSv or more) compared with the least contaminated districts (relative risk [RR] in Belarus 2.24, 95% confidence interval [CI] 1.51–3.32 and in Ukraine 1.78, 95% CI = 1.08–2.93). The increase, though based on a relatively small number of cases, appeared approximately 10 years after the accident, was highest among women who were younger at the time of exposure and was observed for both localised and metastatic diseases. It is unlikely that this excess could be entirely due to the increased diagnostic activity in these areas. © 2006 Wiley‐Liss, Inc.
The process and promise of mental health augmentation of nurse home‐visiting programs: Data from the Louisiana Nurse–Family Partnership
Authors: Boris Neil W. , Larrieu Julie A. , Zeanah Paula D. , Nagle Geoffrey A. , Steier Alison , McNeill Patricia 
Published: February 2006

AbstractThe Nurse–Family Partnership (NFP) model is a well‐studied and effective preventive intervention program targeting first‐time, impoverished mothers and their families. Data documenting the negative impact of maternal depression and partner violence on the developing young child can be used to make a strong case for augmenting NFP programs to focus on mental health problems impacting the mother–child relationship. This article reviews the rationale for and process of augmenting an NFP program in Louisiana. Data on the prevalence of depression and partner violence in our sample are presented alongside a training protocol for nurses and mental health consultants designed to increase the focus on infant mental health. The use of a weekly case conference and telephone supervision of mental health consultants as well as reflections on the roles of the mental health consultant and the nurse supervisor are presented.
Collageneous matrix coatings on titanium implants modified with decorin and chondroitin sulfate: Characterization and influence on osteoblastic cells
Authors: Bierbaum Susanne , Douglas Timothy , Hanke Thomas , Scharnweber Dieter , Tippelt Sonja , Monsees Thomas K. , Funk Richard H. W. , Worch Hartmut 
Published: February 2006

AbstractStudies in developmental and cell biology have established the fact that responses of cells are influenced to a large degree by morphology and composition of the extracellular matrix. Goal of this work is to use this basic principle to improve the biological acceptance of implants by modifying the surfaces with components of the extracellular matrix (ECM), utilizing the natural self‐assembly potential of collagen in combination with further ECM components in close analogy to the situationin vivo. Aiming at load‐bearing applications in bone contact, collagen type I in combination with the proteoglycan decorin and the glycosaminoglycan chondroitin sulfate (CS) was used; fibrillogenesis, fibril morphology, and adsorption of differently composed fibrils onto titanium were assessed. Both decorin and CS could be integrated into the fibrils during fibrillogenesis, the amount bound respectively desorbed depending on the ionic strength of fibrillogenesis buffer. Including decorin always resulted in a significant decrease of fibril diameter, CS in only a slight decrease or even increase, depending on the collagen preparation used. No significant changes in adsorption to titanium could be detected. Osteoblastic cells showed different reactions for cytoskeletal arrangement and osteopontin expression depending on the composition of the ECM, with CS enhancing the osteoblast phenotype. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Enhanced osteogenic promotion around dental implants with synthetic binding motif mimicking bone morphogenetic protein (BMP)‐2
Authors: Seol Yang‐Jo , Park Yoon‐Jeong , Lee Sang‐Cheol , Kim Kyoung‐Hwa , Lee Jue‐Yeon , Kim Tae‐Il , Lee Yong‐Moo , Ku Young , Rhyu In‐Chul , Han Soo‐Boo , Chung Chong‐Pyoung 
Published: February 2006

AbstractSynthetic receptor binding motif mimicking bone morphogenetic protein‐2 (BMP‐2) was covalently linked to titanium (Ti) surfaces through a chemical conjugation process. The composition and properties of surface‐modified Ti were investigated by XPS as well as by measuring surface radioactivity. In vitro tests were conducted with osteoblast‐like MC3T3‐E1 cells to assess cell attachment, morphology, and expression of osteogenic marker in the cells grown on modified Ti surfaces. In addition, in vivo experiments involved implants in mandibular bone defects of beagles to evaluate the effect of surface modification on bone regeneration. Results of XPS measurements showed a complete and homogeneous peptide overlayer on the Ti surfaces; the content was further measured by gamma counting. Biological evaluations showed that the biochemically modified Ti samples were active in terms of cell attachment behavior. The MC3T3‐E1 cell growth rate, marker protein expression, and alkaline phosphatase production of the peptide‐modified surfaces were all higher than those of control Ti. Importantly, the implants in the canine mandibles showed significant increase of bone growth when modified with bioactive peptide, thereby confirming that biochemical modifications of Ti surfaces can enhance the rate of bone healing as compared with untreated Ti surfaces. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006
Changes in color and translucency of porcelain‐repairing resin composites after thermocycling
Authors: Choi Mi‐Sook , Lee Yong‐Keun , Lim Bum‐Soon , Rhee Sang‐Hoon , Yang Hyeong‐Cheol , Lim Young‐Jun 
Published: February 2006

AbstractThe objective of this study was to determine the changes in color and translucency of dental porcelain‐repairing resin composites compared to dental porcelain after thermocycling. Color and spectral reflectance of three shades (A2, A3, and A3.5) of one brand of dental porcelain and three basic shades (A2, A3, and A3.5) and three combinations (A2/A3, A3/3.5, and A2/A3.5) of three brands of porcelain‐repairing resin composites (ABT, FSP, and TCR) were measured, before and after thermocycling for 3000 cycles, relative to the illuminant D65. The specimen was 2 mm in thickness, and 1 mm of each shade was layered to make combined shades. Changes in color (ΔE*ab) and translucency parameter (ΔTP) were calculated. A general linear model by the material (porcelain or resin composite) and shade was used to compare differences (α = 0.05). The range of color changes was 0.68–1.67 in porcelain, 0.56–1.30 in ABT, 2.28–3.10 in FSP, and 0.36–1.15 in TCR. The range of ΔTP was 0.45–0.96 in porcelain, −0.48 to 0.94 in ABT, −1.31 to 0.82 in FSP, and −0.51 to 1.91 in TCR. After thermocycling, changes in color and TP were correlated with the shade of the material, but not with the material. The discrepancy in the changes of color and translucency after thermocycling between porcelain and porcelain‐repairing resin composites should be considered when selecting repairing materials. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
New concept of resin‐dentin interfacial adhesion: The nanointeraction zone
Authors: Koshiro Kenichi , Sidhu Sharanbir K. , Inoue Satoshi , Ikeda Takatsumi , Sano Hidehiko 
Published: February 2006

AbstractThe purpose of this study was to evaluate the morphological appearance of resin‐dentin interface produced by 1‐step adhesive systems in order to classify them, using TEM. It was hypothesized that all interfaces produced by each 1‐step adhesive show characteristics similar to that produced by the conventional self‐etching system rather than that produced by etch‐and‐rinse system. The flattened dentin surfaces were subjected to bonding treatment using either a 2‐step adhesive: single bond, mega bond, unifil bond; or a 1‐step adhesive: Prompt L‐Pop, absolute, xeno CF, AQ bond, one‐up bond F, G bond, and SSB‐200. A flowable resin was then applied to these surfaces. The results revealed that resin‐dentin interfaces produced by 1‐step systems were classified into three categories. Additionally, the interface produced by G bond and SSB‐200 showed a different morphological appearance from that of the others. The interface produced by these adhesives was thinner and less demineralized, with the presence of hydroxyapatite‐coated collagen fibrils. It is proposed that “Nanointeraction Zone (NIZ)” is a new complementary classification for partially demineralized resin‐dentin interdiffusion zones. Thus, one‐step self‐etching adhesive systems can be classified into three categories, etch‐and‐rinse type, mild self‐etching type, and NIZ type, by their morphological appearance of the interface. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Contemporary alumina‐on‐alumina total hip arthroplasty performed in patients younger than forty years: A 5‐year minimum follow‐up study
Authors: Yoo Jeong Joon , Kim Young‐Min , Yoon Kang Sup , Koo Kyung‐Hoi , Kim Jong Won , Nam Kwang Woo , Kim Hee Joong 
Published: February 2006

AbstractCeramic‐on‐ceramic bearing surfaces in total hip arthroplasties (THAs) are an attractive alternative coupling and may offer a promising option for young active patients. We evaluated the clinical results of 72 contemporary primary cementless alumina‐on‐alumina THAs performed in 61 patients younger than 40 years (mean age, 30 years), after a 5‐year minimum follow‐up. Most patients (97%) were able to return to their prearthritic activity levels, and none changed occupation because of a postoperative hip joint problem. No implant loosened radiographically and no stem or cup was revised. Ceramic wear was undetectable in 24 hips, where differentiation of the femoral head from the cup was possible on radiographs and no osteolysis was observed. One ceramic fracture following a major motor vehicle accident and a recurrent subluxation‐associated ceramic liner edge fracture attributed to inadequate hip muscle tone occurred. We believe that this alternative articulation offers a promising option for young active patients. However, our findings also suggest that contemporary ceramic implant fracture is not a potential, but a real danger. Careful preoperative patient evaluation, proper indications for ceramic bearings, and meticulous surgical techniques are recommended to minimize the possibility of modern ceramic failure. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Tissue engineering of articular cartilage with autologous cultured adipose tissue‐derived stromal cells using atelocollagen honeycomb‐shaped scaffold with a membrane sealing in rabbits
Authors: Masuoka Kazunori , Asazuma Takashi , Hattori Hidemi , Yoshihara Yasuo , Sato Masato , Matsumura Kouji , Matsui Takemi , Takase Bonpei , Nemoto Koichi , Ishihara Masayuki 
Published: February 2006

AbstractAdipose tissue derived stromal cells (ATSCs), which were isolated from adipose tissue of rabbit, have shown to possess multipotential, that is, they differentiate into osteoblasts and adipocytes in plate‐culturing and into chondrocytes in an established aggregate culture using defined differentiation‐inductive medium. The aim of this study was to evaluate the utility of ATSCs in tissue engineering procedures for repair of articular cartilage‐defects using the atelocollagen honeycomb‐shaped scaffold with a membrane sealing (ACHMS‐scaffold). We intended to repair full‐thickness articular cartilage defects in rabbit knees using autologously cultured ATSCs embedded in the ACHMS‐scaffold. ATSCs were incubated within the ACHMS‐scaffold to allow a high density and three‐dimensional culture with control medium. An articular cartilage defect was created on the patellar groove of the femur, and the defect was filled with the ATSCs‐containing ACHMS‐scaffold, ACHMS‐scaffold alone, or empty (control). Twelve weeks after the operation, the histological analyses showed that only the defects treated with the ATSCs‐containing ACHMS‐scaffold were filled with reparative hyaline cartilage, highly expressed Type II collagen. These results indicate that transplantation of autologous ATSCs‐containing ACHMS‐scaffold is effective in repairing articular cartilage defects. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
Gene expression profiling of androgen receptor antagonists in the rat fetal testis reveals few common gene targets
Authors: Mu Xueyan , Liu Kejun , Kleymenova Elena , Sar Madhabananda , Young S. Stanley , Gaido Kevin W. 
Published: February 2006

AbstractThe androgen receptor (AR) is expressed in the fetal testis; however, the role of AR in fetal testicular development is poorly understood. Disrupted AR activity and subsequent gene expression alterations may disturb developmental programming of the fetal testis and result in testicular abnormalities later in life. The present study was performed to examine global gene expression patterns in rat fetal testis following in utero exposure to various AR antagonists. Pregnant Sprague‐Dawley rats were treated with flutamide (50 mg/kg/day), linuron (50 mg/kg/day), vinclozolin (200 mg/kg/day), p,p′‐DDE (100 mg/kg/day) or corn oil vehicle by gavage daily from gestation day (GD) 12 –19. Testes were isolated on GD 19, and AR immunostaining, histology, and global changes in gene expression were determined. There were no alterations in the pattern or expression level of AR and no apparent histological changes in the fetal testes in any treatment group. Microarray analysis using Dunnett's test with multiple testing correction revealed no significant gene expression alterations following exposure to flutamide, linuron, vinclozolin, and p,p′‐DDE. A less stringent analysis yielded some chemical specific effects on gene expression, and these effects were further evaluated by real‐time RT‐PCR. Vinclozolin treatment reduced the expression of several genes involved in cholesterol biosynthesis, though the testosterone levels were unchanged in the fetal testes in any treatment group. In flutamide, linuron, and p,p′‐DDE treatment groups, the expression of hemoglobin Y, beta‐like embryonic chain (Hbb‐y) was reduced. Myomesin 2 (Myom2) expression was increased following linuron treatment. Given the lack of a common set of genes and the absence of overt histopathology, we conclude that the fetal testis is not a major target for AR activity at this stage of development although some cell‐type specific gene expression changes cannot be ruled out. © 2006 Wiley Periodicals, Inc. J Biochem Mol Toxicol 20:7–17, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20110
Splicing of scorpion toxin gene BmKK2 in HEK 293T cells
Authors: Zhijian Cao , Chao Dai , Shijin Yin , Yingliang Wu , Jiqun Sheng , Yonggang Sha , Wenxin Li 
Published: February 2006

AbstractUsing GFP as a reporter gene, splicing of scorpion toxin gene BmKK2 was investigated in cultured HEK 293T cells. The results of RT‐PCR and western blotting showed that BmKK2's intron could be recognized and spliced in cultured HEK 293T cells. At the same time, a cryptic splicing site of BmKK2 gene was found at the 91st nucleotide site of the second exon, which is a typical form of alternative splicing. For the first time, alternative splicing would partially explain the diversity of scorpion toxins at the gene level. Moreover, replacing BmKK2's intron with BmP03's intron (an artificial BmKK2‐BmP03 mosaic gene) did not affect the intron's recognition and splicing, but increased the expression of the toxin‐GFP fusion protein by fluorescence imaging, which indicated that both introns may regulate the expression of toxin‐GFP fusion protein. The artificial BmKK2‐BmP03 mosaic gene was also spliced into two kinds of mRNA molecules, which showed that sequence of intron was not absolutely conserved. The results suggested that introns of scorpion toxin genes BmKK2 and BmP03 increase the diversity of scorpion toxins and regulate the expression of their genes. © 2006 Wiley Periodicals, Inc. J Biochem Mol Toxicol 20:1–6, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20111