bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intranasal bivalent vaccine with recombinant Influenza Virus expressing Pneumococcal Surface Protein A (PspA) protects against Streptococcus pneumoniae and Influenza A virus infection in mice
Authors: Araujo Marcio , Cardoso Kimberly , de Souza Lara Alves , Santos Beatriz Silva , de Carvalho Ketyllen Andrade , Messias Sarah Silva , Gonçalves Ana Paula de Faria Gonçalves , Kano Flora Satiko , Alves Pedro , Campos Marco Antônio Silva , Xavier Marcelo Pascoal , Garcia Cristiana Couto , Russo Remo , Gazzinelli Ricardo , Costa Érica Azevedo , Martins Nelson Silva , Miyaji Eliane , Machado Alexandre 
Published: April 2024

Abstract

Streptococcus pneumoniae and influenza A virus (IAV) are significant agents of pneumonia cases and severe respiratory infections globally. Secondary bacterial infections, particularly by Streptococcus pneumoniae are common in IAV-infected individuals, leading to critical outcomes. Despite reducing mortality, pneumococcal vaccines have high production costs and are serotype specific. The emergence of new circulating serotypes has led to the search for new prevention strategies that provide a broad spectrum of protection. In this context, vaccination using antigens present in all serotypes, such as Pneumococcal Surface Protein A (PspA), can offer broad coverage regardless of serotype. Employing the reverse genetics technique, our research group developed a recombinant influenza A H1N1 virus that expresses PspA (Flu-PspA), through replacement of neuraminidase by PspA. This virus was evaluated as a bivalent vaccine against infections caused by influenza A and S. pneumoniae in mice. Initially, we evaluated the Flu-PspA virus ability to infect cells and express PspA in vitro, its capacity to multiply in embryonated chicken eggs and its safety when inoculated in mice. Subsequently, the protective effect against influenza A and Streptococcus pneumoniae lethal challenge infections in mice was assessed using different immunization protocols. Analysis of the production of antibodies against PspA4 protein and influenza, and the binding capacity of anti-PspA4 antibodies/complement deposition to different strains of S. pneumoniae were also evaluated. Our results demonstrate that the Flu-PspA virus vaccine efficiently induces PspA protein expression in vitro, and it was able to multiply in embryonated chicken eggs even without exogenous neuraminidase. The Flu-PspA-based bivalent vaccine was demonstrated to be safe, stimulated high titers of anti-PspA and anti-influenza antibodies, and protected mice against homosubtypic and heterosubtypic influenza A and S. pneumoniaechallenge. Moreover, an efficient binding of antibodies and complement deposition on the surface of pneumococcal strains ascribes the broad-spectrum vaccine response in vivo. In summary, this innovative approach holds promise for developing a dual-protective vaccine against two major respiratory pathogens.
Preparation of composites with MgAl-LDH-modified commercial activated carbon for the quick removal of Cr from aqueous solutions.
Authors: Sun Ying , Wang Zexu , Zhang Shijie , Liu Chuyin , Xu Yunfeng 
Published: April 2024

Abstract

The problem of soil and water contamination caused by Cr discharged from dyeing, electroplating and metallurgical industries has become increasingly serious, which is a potentially great threat to the environment and public health. Therefore, it is essential to develop a quick, efficient and low-cost adsorbent for the remediation of Cr-contaminated wastewater. In this work, we successfully prepared MgAl-LDH/ commercial activated carbon nanocomposites (LDH-CAC) with hydrothermal. The effects of activated carbon type and loading on LDH-CAC were investigated. Moveover, we also have examined the adsorption capacity and adsorption behavior of LDH-CAC on Cr(VI), and proposed a potential removal mechanism. Lastly, the reusability of LDH-CAC was researched. The results showed that the prepared LDH-CAC@1 had the best removal performance at the commercial activated carbon type of CAC-103 and activated carbon loading of 1.0 g. The adsorption behavior study, on the other hand, indicated that LDH-CAC @1 could reach adsorption equilibrium within 10 min with a maximum adsorption capacity of 116.7 mg/g, which was significantly better than MgAl-LDH (10.7 mg/g) and CAC (21.3 mg/g). The primary removal mechanisms included adsorption-coupled reduction, ion exchange, surface precipitation, and electrostatic attraction. Reusability experiment revealed that the removal efficiency of LDH-CAC@1 decreased by 25.1% after four cycles, which illustrated the promising reusability of LDH-CAC@1.This study provides an effective adsorbent with a remarkably fast reaction, which has positive environmental significance for the treatment of Cr (VI) wastewater.
Performance evaluation of standardized residency training based on non-parametric intelligent group decision method
Authors: Zhou Rui , Xu Dandan , Ma LiJuan , Wang Xiaoying , Xu JiaLi , Song Qiwei , Xia Qun 
Published: April 2024

Abstract

Background
This study focuses on evaluating the performance of standardized residency training using a non-parametric intelligent group decision method.
Methods
In this study, a non-parametric intelligent group decision method is designed to create evaluation models for the performance of two hundred pediatric residents undergoing standardized training skills assessment in Anhui Province,China. The evaluation process involves analyzing six indicators: cardiopulmonary resuscitation, humanistic communication, history taking, physical examination, medical record writing, and case analysis.
Results
The two models exhibit consistency in both data and performance levels, validating the scientific feasibility of the new method. The empirical analysis highlights shortcomings in residents' medical record writing and case analysis abilities. Enhancing competency in these areas emerges as a crucial focus in standardized residency training.
Conclusion
The application of non-parametric intelligent group decision-based performance evaluation outperforms relying solely on multiple examiner scores in standardized residency training. This approach effectively identifies individual trainees' strengths and weaknesses, offers improvement recommendations, and supports decisions for disciplinary training base advancement.
Effect of intraoperative cold solution irrigation to reduce postoperative pain in knee osteoarthritis patients who underwent unilateral primary total knee arthroplasty: A double-blinded randomized controlled trial
Authors: Charoenwisetsin Suwis , Jiranantarat Vorakran , Hirunyachoke Paphon , Udomkiat Pacharapol 
Published: April 2024

Abstract

Purpose
To compare the postoperative pain score, opioid consumption, and blood loss in knee osteoarthritis patients who underwent unilateral primary total knee arthroplasty with and without intraoperative cold solution irrigation.
Method
In total, 70 knee osteoarthritis patients were randomly included in the study and allocated into 2 groups. The first group was irrigated intraoperatively with a cold solution and the second group was irrigated intraoperatively with a room-temperature solution.
Results
The cold solution group showed significantly lower pain scores (numerical rating scale, NRS) at 28 hours postoperatively (p = 0.047). There were no significant differences in opioid consumption or blood loss between the groups.
Conclusions
Intraoperative cold solution irrigation in unilateral primary total knee arthroplasty patients may provide the benefit of early postoperative pain reduction for up to 28 hours but has no effect in terms of reducing opioid consumption or blood loss.
Fine Mapping and Identification of the Downy Mildew Resistance Gene BoDMR2 in Cabbage (Brassica oleracea L. var. capitata)
Authors: Wu Yuankang , Zhang Bin , Yang Limei , Zhuang Mu , Lv Honghao , Wang Yong , Ji Jialei , Hou Xilin , Zhang Yangyong 
Published: April 2024

Abstract

Background
Cabbage (Brassica oleracea L. var. capitata) is a crucial Brassica oleracea crop and is widely cultivated worldwide. In recent years, outbreaks of downy mildew caused by Hyaloperonospora parasitica have led to significant field losses in cabbage production. Thus far, there have been few reports on genes related to resistance to downy mildew in cabbage.
Results
In this study, sister lines exhibiting significant differences in disease resistance and susceptibility were identified. Using bulked segregant analysis followed by sequencing (BSA-seq) and linkage analysis, we precisely mapped the adult-stage cabbage resistance gene BoDMR2 to an ~ 300 kb interval on chromosome 7. Among the candidate genes, several single nucleotide polymorphisms (SNPs) and a 3-bp insertion were discovered in the conserved domain of Bo7g117810 in susceptible materials. Furthermore, quantitative real-time polymerase chain reaction (qRT‒PCR) analysis revealed that the expression level of the Bo7g117810 gene in resistant specimens was 2.5-fold greater than that in susceptible specimens. An insertion‒deletion (InDel) marker was subsequently designed based on the identified insertion in susceptible materials, facilitating the identification and selection of downy mildew-resistant cabbage cultivars.
Conclusions
This study identified BoDMR2 as a potential candidate gene associated with adult downy mildew resistance in cabbage, primarily based on differences observed in its gene sequence and expression levels. Additionally, an InDel marker was developed utilizing its mutation, thus offering valuable resources for cabbage resistance breeding
A Proteomic Study to Elucidate Molecular Relationships Between Iron, Oxidative Stress and Polyphosphate in Streptomyces coelicolor A3(2)
Authors: Yılmaz Şerif , Yeşilırmak Filiz , Tunca Sedef 
Published: April 2024

Abstract

Polyphosphate (polyP) is an important energy and phosphate storage polymer in all organisms. Deletion of the polyP synthesising enzyme, polyP kinase (PPK), resulted in an antibiotic overproducing phenotype in Streptomyces. However, the industrial use of overproducing Streptomyces strains without PPK activity (∆ppk) is hampered by their increased sensitivity to oxidative stress. Iron plays a key role in the bacterial response to oxidative stress, and it is also an essential element for various processes in the cell. Conversely, polyP can sequester iron, reducing its bioavailability. This study aimed to elucidate the metabolic relationship between oxidative stress, iron, and polyP metabolisms in Streptomyces coelicolor as an example of the communication of cellular regulatory signalling networks. Comparative proteomic analyses were performed on three biological replicates of wild-type and ∆ppk strains grown in iron-containing and iron-free media. Independent of iron, the results show that the absence of polyP significantly alters the total proteome, revealing the importance of this polymer in maintaining cellular metabolism. The mutant strain was found to have difficulties coping with the iron even in the nutrient-rich medium. Compared to the wild type in the iron-free medium, a general abundance of proteins related to energy metabolism, and protein folding was observed in ∆ppk. In the presence of iron, the expression of the proteins involved in translation, phosphate metabolism and the antioxidant system was increased in the mutant strain compared to the wild type. To our knowledge, this is the first study to clarify the relationship between iron, oxidative stress, and polyphosphate.
Music Communicates Social Emotions: Evidence from 750 music excerpts
Authors: Pring Elliot , Olsen Kirk N. , Mobbs Anthony E. D. , Thompson William Forde 
Published: April 2024

Abstract

Humans perceive a range of basic emotional connotations from music, such as joy, sadness, and fear, which can be decoded from structural characteristics of music, such as rhythm, harmony, and timbre. However, despite theory and evidence that music has multiple social functions, little research has examined whether music conveys emotions specifically associated with social status and social connection. This investigation aimed to determine whether the social emotions of dominance and affiliation are perceived in music and whether structural features of music predict social emotions, just as they predict basic emotions. Participants (n = 1513) listened to subsets of 750 music excerpts and provided ratings of energy arousal, tension arousal, valence, dominance, and affiliation. Ratings were modelled based on ten structural features of music. Dominance and affiliation were readily perceived in music and predicted by structural features including rhythm, harmony, dynamics, and timbre. In turn, energy arousal, tension arousal and valence were also predicted by musical structure. We discuss the results in view of current models of music and emotion and propose research to illuminate the significance of social emotions in music.
Screening of the lipid-lowering probiotic Lactiplantibacillus plantarum SDJ09 and its anti-obesity mechanism
Authors: Yang Baoxin , Wang Wenxuan , Jian Cuiwen , Lv Beibei , He Hailin , Wang Miao , Li Shubo , Guo Yuan 
Published: April 2024

Abstract

In this study, 39 strains of lactic acid bacteria were screened from several fermented foods. Based on the evaluation of functional and prebiotic properties, Lactiplantibacillus plantarum SDJ09 was selected as a promising candidate. It gave a 48.16% cholesterol reduction and 33.73% pancreatic lipase inhibition in cells; exhibited high resistance to acid, bile salts, and gastrointestinal fluid; and had strong antibacterial activity and high adhesion capabilities. More importantly, the lipid-lowering effect of L. plantarum SDJ09 was also investigated using 3T3-L1 mature adipocytes and HepG2 nonalcoholic fatty liver disease models. L. plantarum SDJ09 effectively decreased triglyceride accumulation by more than 50% in both cell models, in which the expression of PPARγ, C/EBPα, aP2, and LPL in 3T3-L1 cells was significantly downregulated by L. plantarum SDJ09. L. plantarum SDJ09 also improved lipid metabolism by downregulating the expression of HMGCR, SREBP-1c, ACC, and FAS and upregulating the expression of CYP7A1 in HepG2 nonalcoholic steatohepatitis cells. Therefore, L. plantarum SDJ09 has the potential to effectively decrease obesity and non-alcoholic fatty liver disease (NAFLD) by inhibiting lipid accumulation, providing a prospective probiotic agent for anti-obesity.
Amelioration of fat clay treated with cohesive and cohesion less soils
Authors: Farooq Khalid , Mujtaba Hassan , Ashiq Syed Zishan , Shah Mudassar Munir , Mohamed Abdullah 
Published: April 2024

Abstract

The present study is aimed to evaluate the amelioration of fat clay by blending it with cohesive non-swelling soil (CNS) and cohesionless silty sandy soil (termed as Kassu). Fat clay sample with liquid limit (LL) 50, plasticity index (PI) 26 was collected from a site located near Narowal city while CNS and Kassu samples were procured from sites located at outskirts of Lahore (Pakistan). Geotechnical parameters evaluated after conducting tests on virgin soil indicated it as unsuitable soil for construction purposes. A series of laboratory tests were performed after blending fat clay with CNS and Kassu in different proportions ranging between 0 ~ 35% with 5%intervals. The laboratory tests including modified Proctor compaction, unconfined compression, California bearing ratio (CBR)and one-dimensional consolidation tests in addition to classification tests were performed on virgin and blended samples. The LL decreased from 50–32% and PI reduced from 24 to 13 with 35% addition of CNS while for Kassu LL and PI reduced to 29% and 12, respectively. CBR value of blended samples increased from 4–7% making the blended soil an acceptable subgrade for roads and foundation construction. Also, swell potential reduced from 4–1.2% ~ 0.26% for blended samples. Regression models have been proposed to predict swell pressure and ultimate swell potential of CNS and Kassu-treated swelling clays. Based on the study, it was concluded that significant improvement in mitigating expansive characteristics of fat clay can be achieved by blending it with CNS and Kassu. Meanwhile, CNS is observed to be more effective as compared to Kassu in controlling the swell properties of the fat clay.
Cross-cultural adaptation of the Quebecois Patient-Centered Coordination by a Care Team Questionnaire for use in France
Authors: Piraux Arthur , Poitras Marie-Eve , Lemarchand Sandra , Sidorkiewicz Stephanie , Ramond-Roquin Aline 
Published: April 2024

Abstract

Background
The prevalence of chronic disease and multimorbidity is increasing and the associated disease and treatment burden is particularly heavy. Coordinated multidisciplinary, patient-centered care is particularly important for people living with chronic disease or multimorbidity. There was no valid tool to measure the quality of coordinated patient-centered care from the patient’s perspective until the Patient-Centered Coordination by a Care Team (PCCCT) questionnaire was recently developed in Canada (Quebec/Ontario). The Quebecois version has been validated but is not directly transferable to France due to linguistic, cultural and health system differences between the two countries. To perform cross-cultural adaptation of the Quebecois PCCCT questionnaire to obtain a new version adapted for use in France ensuring item and semantic equivalence between the two versions.
Methods
The adaptation process consisted of two stages, both of which were supervised by a scientific committee made up of five healthcare professionals. The first stage was a Delphi consensus involving a multidisciplinary healthcare professional panel to evaluate and harmonize the clarity and appropriateness of the questionnaire for patients in the French health system. During the second stage, adult patients with one or more chronic diseases, from various age, sex, socio-occupational categories assessed the comprehensibility and conformity of the adapted version of the questionnaire resulting from stage 1 and improved it if necessary. This was achieved using cognitive interviews.
Results
During Stage 1, two rounds were undertaken with 10 professional experts resulting in consensual reformulation of 10 out of the 14 items. These newly formulated items and the 4 remaining items were submitted to patients in Stage 2. Cognitive interviews were undertaken with 14 patients, testing 3 successively adapted versions of the questionnaire, until three consecutive patients did not find any ambiguity or misunderstanding. This final version resulting from the cross-cultural adaptation process is usable in France and has item and semantic equivalence to the original Quebecois version.
Conclusions
This French version is a useful resource for the health system reforms aimed at promoting more integrated and patient-centered care pathways. Measurement equivalence will be addressed in a future study.
Trial registration:
Not applicable.
I was like sh*t this is gonna hurt’: Implementing Self-Sampling of Dried Blood Spots to Measure HIV Viral Load
Authors: Chavez Jennifer V. , Ewart Leah Davis , Ilyas Ozair , Ghanooni Delaram , Diaz José E. , Atkins Lindsay , Ramos Richard , Garayua Adriana Hernandez , Stewart Alex , Horvath Keith J. , Hirshfield Sabina , Carrico Adam W. 
Published: April 2024

Abstract

Background
Sexual minority men (SMM) with HIV who use stimulants may experience greater difficulties with antiretroviral therapy adherence which amplifies risk for unsuppressed HIV viral load (VL). Remote monitoring of VL could support efforts to rapidly respond to sub-optimal adherence.
Methods
This qualitative study enrolled 24 SMM with HIV who use stimulants to examine experiences with two different dried blood spots (DBS) self-sampling devices (i.e., Tasso-M20 vs. HemaSpot HD) to measure VL. Participants were asked to complete self-sampling of DBS using both devices, and then participated in a 45-minute semi-structured interview. Interviews focused on ease of use, device preference, experiences with receiving and mailing kits, and barriers to participating in research. A thematic analysis was conducted to analyze interviews transcripts.
Results
Twenty-two participants (92%) returned the Tasso-M20 and 21 (88%) returned the Hemaspot HD devices. Among the 22 participants that completed qualitative interviews, twenty-three codes were identified and collapsed within seven themes. Preferences for devices were based on convenience, pain and prior experiences with finger-pricking technology. Participants emphasized that clearer instructions with contingency plans for self-sampling of DBS would improve the user experience with self-sampling of DBS. Intersectional stigma (e.g., HIV, sexual minority status, and substance use) was noted as an important consideration in implementing self-sampling of DBS. Promoting decision making, or the option to choose sampling method based on personal preferences, may improve engagement and likelihood of DBS completion.
Conclusions
Findings will guide the broader implementation of self-sampling of DBS to optimize VL monitoring in SMM with HIV who use stimulants.
Exploring the roles of paradoxical tensions, paradoxical thinking, and team psychological capital on the creativity of engineering university students
Authors: guo huifen , zhou zhen , ma fengqi 
Published: April 2024

Abstract

Background The multifaceted challenges encountered by engineering university students generate paradoxical tensions, which serve as catalysts for fostering creativity. Engaging in paradoxical thinking during academic pursuits enhances the ability of students to solve complex engineering problems. Despite this, the intricate interconnections among paradoxical tensions, paradoxical thinking, and the creativity of engineering university students remain ambiguous.
Methods This study sought to address this gap by surveying 1410 engineering university students in China, delving into how paradoxical thinking mediates the link between paradoxical tensions and creativity. Additionally, it investigated the moderating impact of team psychological capital on the associations between paradoxical tensions and both paradoxical thinking and creativity. SPSS 24.0 was initially used to convert the cleaned data into a “.csv” format, and Smart PLS (v.4.0.9.5) was then employed to assess the model.
Results The findings of the study reveal a positive influence of paradoxical tensions on both creativity and paradoxical thinking. Notably, paradoxical thinking emerges as a significant contributor to enhancing the creativity of engineering university students. Furthermore, the findings show that paradoxical tensions enhance creativity by influencing paradoxical thinking. While team psychological capital emerged as a significant factor in moderating the link between paradoxical tension and creativity, its role in moderating the association between paradoxical tension and paradoxical thinking was not statistically significant.
Conclusions This study revealed how paradoxical tensions among engineering university students influence creativity through paradoxical thinking, moderated by team psychological capital. The findings not only provide new insights for researchers to better understand paradoxical tensions, paradoxical thinking, team psychological capital and the underlying psychological mechanism for engineering university students' creativity, but also have practical implications for education administrators.