bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Practical Guide to Whole Genome Sequencing in the NICU
Authors: Seither Katelyn , Thompson Whitney , Suhrie Kristen 
Published: March 2024

Abstract
The neonatal period is a peak time for the presentation of genetic disorders that can be diagnosed using whole genome sequencing (WGS). While any one genetic disorder is individually rare, they collectively contribute to significant morbidity, mortality, and health-care costs. As the cost of WGS continues to decline and becomes increasingly available, the ordering of rapid WGS for NICU patients with signs or symptoms of an underlying genetic condition is now feasible. However, many neonatal clinicians are not comfortable with the testing, and unfortunately, there is a dearth of geneticists to facilitate testing for every patient that needs it. Here, we will review the science behind WGS, diagnostic capabilities, limitations of testing, time to consider testing, test initiation, interpretation of results, developing a plan of care that incorporates genomic information, and returning WGS results to families.
Group A Streptococcus Infections
Authors: Bhavsar Sejal Makvana 
Published: March 2024

Abstract
Group A Streptococcus causes a variety of clinical manifestations, including pharyngitis and skin and soft tissue infections as well as more invasive disease. There are also multiple nonsuppurative complications of group A Streptococcus infection, including acute rheumatic fever and poststreptococcal glomerulonephritis. Pediatricians should be able to diagnose and treat the various presentations of the infection.
Síntesis de tioureas con isoniacida y niacida estructural: test frente a bacterias Gram + y Gram –
Authors: Tovar-Taboada Carlo Felipe , Angulo-Cornejo Jorge 
Published: March 2024

Se han sintetizado los ligandos N-(2-nicotinoilhidrazina-1-carbonotioil)benzamida (H3L1) y su isómero N-(2-isonicotinoilhidrazina-1-carbonotioil)benzamida (H3L2) tipo N, S, O dadores, por reacción del isotiocianato de benzoilo con la hidracida nicotínica y la isoniacida respectivamente. Los ligandos H3L1 y H3L2 fueron caracterizados por 1H-RMN ,13C-RMN, FT-IR y análisis elemental. Se ha realizado el análisis computacional utilizando las funciones de Fukui para determinar las razones por la cuales no se formaron los 1,2,4-triazoles. Se han realizado pruebas preliminares de los ligandos sintetizados frente a Staphylococcus aureus (Gram+) y Escherichia coli (Gram-), habiéndose demostrado importante actividad frente las bacterias mencionadas.
DINAMIKA POPULASI IKAN KAKAP MERAH (Lutjanus malabaricus) di PERAIRAN PINRANG
Authors: Rapi Nuraeni L , Djumanto Djumanto , Murwantoko Murwantoko 
Published: March 2024

Ikan kakap merah merupakan salah satu komoditas perikanan yang bernilai tinggi sebagai ikan konsumsi. Pengetahuan sebaran panjang, umur, pertumbuhan, dan mortalitas digunakan untuk mengetahui kondisi stok ikan. Penelitian ini bertujuan untuk menganalisis pertumbuhan, mortalitas, laju eksploitasi dan pola rekrutmen ikan kakap merah (Lutjanus malabaricus). Sampling dilakukan tiap bulan sekali dari April 2019 sampai Maret 2020 di perairan Kabupaten Pinrang. Contoh ikan diperoleh dari hasil tangkapan nelayan gill net,rawai dasar dan bubu. Tiap contoh ikan dilakukan pengukuran panjang menggunakan penggaris dengan ketelitian 0,1 mm. Data frekuensi panjang dianalisis menggunakan paket program FiSAT II untuk mengetahui sebaran panjang, laju pertumbuhan, mortalitas,tingkat eksploitasi dan pola rekrutmen. Total sampel ikan yang dikumpulkan selama penelitian sebanyak 916 ekor. Nilai L∞ sebesar 72,95 cm, nilai K sebesar 0,41/tahun dan nilai t0 sebesar-0,28. Laju mortalitas total sebesar 1,21, mortalitas alami (M) sebesar 0,5, mortalitas penangkapan (F) sebesar 0,71, dan Tingkat eksploitasi (E) sebesar0,59. Rekrutmen berlangsung tiap bulan dengan persentase tertinggi pada akhir musim hujan, puncak musim kemarau dan awal musim hujan. Perlu pengaturan penangkapan tentang ukuran mata pancing dan mata jaring agar tingkat eksploitasi sumberdaya ikan kakap merah berkesinambungan Red snapper is a fisheries commodity with high value as a potential export. Knowledge of the distribution of length, age, growth, and mortality is used to determine fish stocks' condition. This study analyzes the growth, mortality, exploitation rate, and recruitment pattern of red snapper (Lutjanus malabaricus). Sampling has been carried out every month from April 2019 to March 2020 in Pinrang Regency waters. Fish samples were obtained from the catch of gill net fishermen, bottom longlines, and traps. Each fish sample is measured using a ruler to a length of 0.1 mm. Long frequency data were analyzed using the FiSAT II program package to determine the length distribution, growth rate, mortality, exploitation rate, and recruitment patterns. The total fish samples collected during the study were 916 individuals. The L∞ value is 72.95 cm, the K value is 0.41 / year, and the t0 value is -0.28. The total mortality rate is 1.21, natural mortality (M) is 0.5, fishing mortality (F) is 0.71, and the exploitation rate (E) is 0.59. Recruitment occurs every month with the highest percentage at the end of the rainy season, the dry season's peak, and the beginning of the rainy season. Fishing arrangements are needed about size hook and mesh size so that the level of exploitation of red snapper resources is sustainable.
INDEKS KEANEKARAGAMAN JENIS IKAN DEMERSAL DI LAUT ARAFURA
Authors: Nurulludin Nurulludin , Prihatiningsih Prihatiningsih , Panggabean Anthony Sisco , Taufik Muhammad , Kembaren Duranta Diandria , Mahulette Ralph Thomas , Saimroh Saimroh , Nurdin Erpind 
Published: March 2024

Ikan demersal merupakan salah satu sumber daya yang memiliki potensi sangat tinggi di Laut Arafura. Informasi indeks keanekaragaman ikan demersal diperlukan setelah adanya moratorium pukat tarik di Laut Arafura. Kegiatan penelitian dilaksanakan pada tanggal 20 Oktober sampai 20 November 2018 dengan mengikuti survei sumber daya ikan di Wilayah Pengelolaan Perikanan (WPP) 718 Laut Aru dan Arafura. Analisis data menggunakan beberapa nilai indeks biodiversitas meliputi keanekaragaman jenis (species diversity), kemerataan (species evenness), kekayaan jenis (species richness), dan dominansi jenis (species dominant). Keanekaragaman jenis dibagi dalam 3 (tiga) wilayah penelitian yaitu Timur, Barat dan Utara Laut Arafura. Indeks Keanekaragaman Shannon Wiener (H’) ikan demersal bervariasi antara 1,26 – 2,89. Nilai indeks kekayaan jenis ikan 9,37—16,8, indeks dominansi (D) antara 0,09-0,51. Indeks kemerataan jenis dari Pielou (E) Arafura bagian timur 0,13 dan utara dengan nilai 0,24 dan perairan Arafura bagian barat nilai indeks sebesar 0,27. Nilai indeks kemerataan jenis antara 0,26-0,50. Kondisi tersebut menunjukkan adanya keseimbangan dalam pemanfaatan sumber daya ikan demersal yang ada baik dari segi pemangsaan maupun secara spasial di Laut Arafura.Demersal fish is a resource that has very high potential in the Arafura Sea. Information on the diversity index of demersal fish is needed after the moratorium on trawling in the Arafura Sea. The research activity was carried out from 20 October to 20 November 2018 by participating in a survey of fish resources in the 718 Aru and Arafura Sea Fisheries Management Area (WPP). Data analysis used several biodiversity index values including species diversity, species evenness, species richness, and species dominance. Species diversity is divided into 3 (three) research areas, namely East, West and North Arafura Sea. The Shannon Wiener Diversity Index (H') of demersal fish varies between 1.26 – 2.89. Fish species richness index value is 9.37-16.8, dominance index (D) is between 0.09-0.51. The evenness index of Pielou (E) eastern Arafura is 0.13 and north is 0.24 and the Arafura waters is 0.27. The value of the species evenness index is between 0.26-0.50. This condition shows that there is a balance in the utilization of existing demersal fish resources, both feeding habits and spatial in the Arafura Sea.
The practice of Daylight Saving Time in Canada: Its suitability with respect to sleep and circadian rhythms
Authors: De Koninck Joseph , Nixon Ashley , Godbout Roger 
Published: March 2024

AbstractDaylight Saving Time (DST) is the practice of setting the clocks one hour forward from Standard Time (ST) in the spring and back again to ST in the fall. This commentary discusses the impact of bi-annual time changes on sleep and circadian rhythms and suggests avenues to minimize negative outcomes on the well-being of Canadian citizens. Ideally, ST should be close to solar time, meaning that daylight is equally distributed before and after noon time, i.e., when the sun is at its highest point in the sky. In Canada, some provinces are proposing to opt out of DST to either return to constant ST throughout the year or to implement permanent DST. National and international associations of clinicians and researchers on sleep and biological rhythms and in health sciences have positioned themselves in favour of permanent ST. In Canada, the Canadian Sleep Society and the Canadian Society for Chronobiology have also issued such a position. This commentary focuses on the implications of previous research findings for sleep and health in Canada given its northern geographical location. It concludes with a research agenda focusing on the Canadian context.
The Futility of Language as a Means of Communication in Edward Albee’s Who’s Afraid of Virginia Woolf?, Fam and Yam, and The Sandbox
Authors: Abd-Rabbo Muna M. , Zalloum Ghadir B. , Al-Wahsh Dima M. 
Published: March 2024

The three plays Who’s Afraid of Virginia Woolf? (1962) The Sandbox (1959) and Fam and Yam (1959) all display the absurdist tendencies to dispel the traditional view of language as the rational means of communication amongst mankind. In absurdist drama the world is portrayed as one that is meaningless and incomprehensible to the human mind. Language, which is confined to faulty human perception and subjectivity, is inefficient in the face of an unfathomable universe. A gap persists between the meanings in language and the world it purports to describe. In addition, the lack of fit between what language permits man to say and what he actually wants to say leads to a breakdown in communication and ultimately to the alienation of the individual. In absurdist works this chaotic sphere of existence is reflected in the dispensing of traditional elements in drama as well as in the illogical usage of language by the characters. Thus, absurdist drama acts as a counter-discourse to the previously dominant, essentialist discourse of realist drama. In this article a brief overview of the absurdist depiction of language’s insufficiency in communication is presented to serve as a backdrop for the analysis of certain segments in Albee’s aforementioned plays in order to assess the extent to which Albee’s plays demonstrate the absurdist’s notion of language’s deficiencies. Thus, the sections chosen for discussion are those that showcase language’s apparent shortcomings in generating human contact.
Evaluation of FTIR, Macro and Micronutrients of Compost from Black Soldier Fly Residual: in Context of Its Use as Fertilizer
Authors: Sukamto S. , Rahmat Ali 
Published: March 2024

The extensive use of chemical inorganic fertilizers promotes the degradation of agricultural land quality and leads to various environmental contaminations. Owing to these problems, the alternative way called “organic farming” has been developed through the use of organic fertilizers including compost. In this study, compost product obtained from composting process by using larvae as the biological agent has been prepared. Macronutrients (i.e. N, P, K, Ca, and Mg) and micronutrients (i.e. Mn, Fe, Cu, Zn, and Ni) have been determined using Inductively Coupled Plasma-Optical Emission Spectrometry, Walkey and Black method, Kjeldahl method, and P Bray-1 method, which showed sufficient content and meet the standard value. Analysis and the comparative study showed that the compost product can be used as a fertilizers substitute. The compost from residual offers more economic, great availability, and environmentally friendly fertilizers for agricultural purposes.
Consecutive Purple Sweet Potato Vinegar Fermentation Using Mixed Starter of Aspergillus spp, Saccharomyces Cerevisiae, and Dried Starter of Acetobacter pasteurianus
Authors: Cakrawati Dewi , Hongsprabhas P. 
Published: March 2024

This study investigated the effect of sorbent material on the viability of dried A. pasteurianus starter and the use of defined starter culture of fungi Aspergillus spp, yeast S. cerevisiae, and dried A. pasteurianus starter during purple sweet potato vinegar fermentation. Complex carbohydrate in cultivation media protects cells during drying, while rice flour as sorbent material absorbs water to obtain dried starter of A. pasteurianus. Increasing the ratio of cultivation media and sorbent material from 1:1 to 1:2 could reduce aw of dried A. pasteurianus starter that will prolong storage time. Mixed starter of Aspergillus spp, yeast S. cerevisiae, and dried A. pasteurianus starter could convert starch to glucose to EtOH to acetic acid during fermentation. However, media saccharified by A. usamii contain lower EtOH and higher acetic acid than media saccharified by A. shirousamii (P0.05). It is noteworthy that A. usamii could hydrolyze starch and protein in rice flour to prevent nutrient exhaustion during acetic acid production by A. pasteurianus. While nutrient exhaustion in media saccharified by A. shirousamii leads to the utilization of acetic acid known as over-oxidation.
Safety-centric urban engagement plan
Authors: Kiełek Kamil 
Published: March 2024

Research objective: The objective of this study is to assess the efficacy of a Safety-Centric Urban Engagement Plan (SCUEP) that intends to improve the safety of city centres by actively involving the community. This study investigates the impact of heightened social engagement in public areas on the safety and overall welfare of individuals residing in urban metropolis centres.
Methodology: The research adopts a mixed-methods approach, combining quantitative data analysis of crime rates and qualitative assessments of community perceptions before and after the implementation of SCUEP. It also includes case studies from various municipalities that have adopted similar engagement plans.
Main findings: The results show a direct correlation between heightened social participation and reduced crime rates in city centers. Improved lighting, well-maintained public spaces, and community events correlate with increased public use of city centers and a consequent reduction in crime.
Application of the study: The findings offer practical insights for urban planners, legislators, and law enforcement authorities. The significance of engaging local populations in safety measures is emphasised, indicating that successful urban involvement can result in enduring safety solutions in city centres.
Originality/Novelty of the study: The study brings a novel perspective by linking urban design and social engagement with urban safety. It introduces SCUEP as an innovative framework that integrates community-led activities, urban planning, and law enforcement strategies to foster safe and inclusive city centers.
Implicit Theories in Relationship with Social Judgments and Aggression Behaviors. Evidence Starting from Carol Dweck's Theoretical Framework
Authors: Vechiu Adina-Petronela , Grădinariu Tudorița 
Published: March 2024

Implicit theories of personality fall under social-cognitive theories and underlie studies that have examined the perspectives, beliefs, and judgments that people make about certain phenomena or behaviors. Although the theoretical model of implicit beliefs was originally categorized as theories about the self, the authors Dweck and Leggett (1988) extended it, applying it to the fild of social judgments about the characteristics of other people. In the present paper we aim to illustrate children's and adolescents' entity and incremental beliefs about aggression. In accordance with this purpose, we conducted a theoretical foray into implicit theories and their implications in the area of ​​social judgments in an attempt to capture how implicit beliefs about aggression are projected into the social interactions of children and adolescents. We believe that our remarks and findings can be important resources for educational factors in the effort to improve the effectiveness of programs to prevent aggressive behaviors and attitudes in children and youth.
Digitalization of Educational Activities at the Municipal Level
Authors: Kulchytska Nadiia , Kulchytskyi Ruslan , Dermanska Liudmyla , Semchyshyn Lida , Kostyshyn Nataliia , Yakovets Tetiana 
Published: March 2024

The article examines the activity of the municipality from the point of view of the education management system in modern realities, namely in the conditions of digital transformation of society and its life, in particular, digitalization of educational activities. The main advantages and disadvantages of the digitalization of education are analyzed and presented. In the context of the research, the theoretical, normative and legal aspects of the current educational activity are presented, as a key link in solving problems of any nature for the benefit of the local population, in particular at the municipal level. In conclusion, the priority directions of the development of education management in the conditions of digitalization are given, which should effectively influence the optimization of organizational and management processes in the field of digital transformation, as well as the active penetration of digital technologies into the educational process of various educational organizations.
The article reveals the effectiveness of information and communication technologies in municipal educational institutions training future specialists. In the context of the implementation of the research goal, the role of digitization as a factor in the implementation of educational activities in the process of training specialists in the economic field is studied. In the modern conditions of the development of the information society, there is a need for fast and high-quality calculation of economic indicators. It is this need that prompts the widespread use of software accounting methods. Digitalization has absorbed various fields of human activity.
Cholécystectomie par Célioscopie de Lithiases Biliaires Symptomatiques chez une Jeune Femme de 34 Ans et Revue de la Littérature (Un Cas Clinique)
Authors: Youssouf Oumarou , Diallo Mamadou Sarifou , Mustapha Riadh , Mazlout Chokri , Hjaiej Mourad , Ouimon Daniel Sylvere , Raphaï Clotaire Donatien 
Published: March 2024

Introduction : La lithiase biliaire est une pathologie d’une grande fréquence ; elle est le plus souvent asymptomatique, mais peut se compliquer en impactant négativement la qualité de vie. L’ablation de la vésicule sous endoscopie est prometteuse. Nous rapportons un cas clinique pour discuter des modalités thérapeutiques à travers une revue de la littérature. Nous rapportons, l’observation d’une femme de 34 ans avec antécédent obese Elle nous a consulté en juin 2020 pour des vomissements post prandiaux chroniques avec altération de l’état générale. Les examens paraclinques réalisés n’ont pas révélé une cause évidente. Evacuée à la polyclinique Hammamet à Tunis le diagnostic d’une lithiase vésiculaire a été posé et une ablation sous célioscopie était réalisée avec extraction de 13 gros calculs d’aspect cholestéroliques dont le diamètre moyen de 3 cm, et la pièce opératoire a trouvé un aspect histologique d’une cholécystite chronique diverticulaire en poussée aiguë modérée. La patiente est totalement guérie de sa maladie.

Cholelithiasis is a very common pathology; it is most often asymptomatic, but can become complicated by negatively impacting quality of life. Removal of the gallbladder under endoscopy is promising. We report a clinical case to discuss therapeutic modalities through a review of the literature. We report the observation of a 34-year-old woman with a history of obesity. She consulted us in June 2020 for chronic postprandial vomiting with deterioration in general condition. Paraclinical examinations carried out did not reveal an obvious cause. Evacuated to the Hammamet polyclinic in Tunis, the diagnosis of gallbladder lithiasis was made and an ablation under laparoscopy was carried out with extraction of 13 large cholesterol-looking stones with an average diameter of 3 cm, and the operating specimen found an appearance histology of chronic diverticular cholecystitis in moderate acute attack. The patient is completely cured of her illness. Conclusion: The interest of the work lies in the atypical case of symptomatic cholelithiasis which our technical platform did not allow to easily treat in our country.
Development of an Analytical Method For Determination of Urine 3-Phenylpyruvic Acid Based on Liquid-Liquid Extraction and LC-MS/MS
Authors: CANBAY Erhan , ÇELİK CANBAY Zeynep , YEŞİLYURT Berrak , DOĞAN Serçin , ARABACI İrem , DEMİREL SEZER Ebru , Y. SÖZMEN Eser 
Published: March 2024

Aim: The aim of this study is to develop a rapid, precise, specific LC MS MS method for the determination of 3-phenylpyruvic acid, which has an important place in the diagnosis of phenylketonuria, a metabolic disease resulting from Phenylalanine Hydroxylase Enzyme deficiency.
Materials and Methods : Analytical measurements were made with Acqutiy UPLC MS MS (Waters Xevo TQD). The chromatographic separation was operated on an Acquity UPLC Phenyl column (50 mm × 2.1 mm, 1.7 μm) with gradient elutionusing 0.1% formic acid containing water and methanol as the mobile phase. Within the scope of the study, firstly, sample preparation steps were focused and dispersive liquid liquid extraction and traditional liquid extraction methods were examined. The best results were obtained in the conventional liquid liquid extraction method, in which dichloromethane was used as the extraction solvent. In addition, in this study, trans cinnamic acid was tested as an internal standard for the determination of 3-phenylpyruvic acid and passed the validation steps.
Results : The linear range of the developed LC MS MS method was found to be between 0.009-5 M, while the detection limit was found to be 0.001 M. Intraday repeatability was below 7% for 3 levels, and interday repeatability was below 10%. In the recovery trial, which showed the accuracy of the method, the results in the urine sample for 3 levels were in the range of 97%-103%, and there was no significant ion suppression in the matrix effect trial, which showed how clean the final matrix obtained as a result of the sample preparation steps was.
Conclusion: As a result, a fast, inexpensive LC MS MS method has been developed for 3-Phenylpyruvic acid.