bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract LB198: The PAX5 oncogene encodes circular RNA products involved in B cell cancer processes
Authors: Martin Danick , Hannay Brandon , McGraw Farrah , Martin Alexis , Veilleux Vanessa , Finn Nicholas , Robichaud Gilles A. 
Published: April 2024

Abstract
Introduction: B-cell cancers represent some of the most prevalent hematological lesions in adult and children. B-cell cancers are also notable for their malignancy and resistance to drug therapies. Our group studies B-cell oncogenes and recently discovered that the prominent PAX5 oncogene, which usually encodes transcription factors, also generates non-coding circular RNA transcripts. Circular RNAs (circRNAs) represent a new class of gene products involved in many biological processes and cancer disease. Due to their innate ability to bind and modulate co-interacting proteins and microRNAs (miRs), circRNAs represent new signaling avenues in cancer biology.
Hypothesis: Given that products from the PAX5 oncogene are important regulators of B-cell cancers, we hypothesized that PAX5 circRNAs (circPAX5) are also involved in B-cell oncogenesis.
Objective: We therefore set out to study circPAX5 products, and their co-interacting miRs, in B-cell cancers.
Results: Using a series of B-cell cancer models and clinical samples, we first profiled the expression of circPAX5 products and found that B-cell leukemias overexpress circPAX5 where its predominant circRNA isoform consisted of exons 2 to 5 sequences of the PAX5 locus. To establish a functional role of circPAX5 on cancer processes, we evaluated B-cell proliferation and chemosensitivity in cancer models expressing conditioned levels of circPAX5. Interestingly, we found that the suppression of circPAX5 levels resulted in the alteration of cancer growth rates, which were dependent upon B-cell differentiation state and population subtype. Mechanistically, we also found that circPAX5 interacts with specific miRs, which were profiled by circRNA precipitation and deep sequencing. Selected sponged miRs were then studied by bio-informatic analyses and mapped to downstream mRNA targets and B-cell cancer pathways.
Conclusions: Altogether, our findings uncover PAX5 circular products, which are involved in B-cell cancer processes and could potentially represent a new cancer pathway targeted for therapeutic and/or diagnostic strategies in B-cell cancer lesions.
Citation Format: Danick Martin, Brandon Hannay, Farrah McGraw, Alexis Martin, Vanessa Veilleux, Nicholas Finn, Gilles A. Robichaud. The PAX5 oncogene encodes circular RNA products involved in B cell cancer processes [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB198.
Abstract LB262: Reprogramming the tumor microenvironment by targeting IGF2-IGF1R signaling, enhancing viro-immunotherapy
Authors: Noh Min Hye , Miller Alexandra , Nguyen Grace , Nakashima Hiroshi , Chiocca E. Antonio , Kaur Balveen , Zhao Zhongming , Lee Tae Jin , Yoo Ji Young 
Published: April 2024

Abstract
While an FDA-approved oncolytic herpes simplex-1 virus (oHSV) has shown therapeutic promise with superior safety, accumulating clinical data revealed that its therapeutic efficacy is observed in a small subset of patients. Here, we show that NFκB-mediated Insulin-like Growth Factor 2 (IGF2)/Insulin-like Growth Factor-1 Receptor (IGF1R) signaling pathway as a key modulator for oHSV therapy-mediated tumor resistance. RNA sequencing on the oHSV-infected primary glioblastoma (GBM) and breast cancer (BC) cells identified IGF2 as one of the top 10 upregulated secretomes. Transcriptomic analysis also revealed significant upregulation/enrichment of genes related to IGF2-IGF1R-MAPK pathway in oHSV-treated tumor cells. Infection with oHSV in GBM and BC cells up-regulated IGF2 transcription via direct binding of NFκB in the IGF2 promoter 3. Compromising the IGF2-IGF1R signaling pathway using IGF2 neutralizing antibody significantlDB7 BC tumors in vivo. To circumvent this and promptly translate our findings in clinical settings, we have developed a novel oHSV, oHSV-D11mt, by incorporating modified IGF2R domain 11 into the parental oHSV genome to function as IGF2 decoy receptor as a single-agent modality. oHSV-D11mt specifically bind to IGF2 not IGF1, blocks oHSV-induced IGF2-IGF1R signaling, increases tumor cell killing, decreases oHSV-induced neutrophils/PMN-MDSCs infiltration, immune suppressive/proangiogenic cytokine secretion, and increases CD8+ cytotoxic T lymphocytes (CTLs) activation, resulting in increased virus propagation and mice survival. These findings suggest that IGF2-IGF1R signaling is a novel target to overcome oHSV therapy resistance and oHSV-D11mt could be used for improved viro-immunotherapy.
Citation Format: Min Hye Noh, Alexandra Miller, Grace Nguyen, Hiroshi Nakashima, E. Antonio Chiocca, Balveen Kaur, Zhongming Zhao, Tae Jin Lee, Ji Young Yoo. Reprogramming the tumor microenvironment by targeting IGF2-IGF1R signaling, enhancing viro-immunotherapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB262.
Abstract LB268: Discovery of a potent, selective and brain penetrating PARP1 inhibitor, XZP-7797
Authors: Gao Yeqing , Lu Yuqian , Chen Bo , Jiang Benke , Wang Feng , Zhang Genyan , Ma Xifeng , Zhou Dapeng , Shi Chunmin , Wang Yunling , Liu Zhipeng , Sun Jun , Zhou Huimin , Xu Mei , Li Jiakui , Liu Bin 
Published: April 2024

Abstract
First-generation PARP1/2 inhibitors are approved for the treatment of cancers, such as ovarian, prostate, pancreas, and breast cancers, with maximal activity against tumors harboring BRCA1/2 mutation or homologous recombination deficiency (HRD). However, PARP1/2 inhibitors in clinical use have severe hematological toxicities due to the inhibition of PARP2, which has been shown to play an important role in the survival of hematopoietic progenitor cells. Furthermore, the current data indicate that synthetic lethality with BRCA mutations is primarily caused by PARP1 inhibition and trapping, whereas PARP2 inhibition and trapping are not critical for anticancer activity. Therefore, the development of selective PARP1 inhibitors is expected to reduce the hematological adverse effects associated with PARP2 inhibition while maintaining the required efficacy. XZP-7797 is a potent, selective, and brain-penetrating PARP1 inhibitor with trapping ability. In biochemical assays, XZP-7797 is more than 1000-fold selective for PARP1 over PARP2 and other members of the PARP family, including PARP3, PARP5a, PARP5b, and PARP6. XZP-7797 can significantly inhibit MDA-MB-436 cell proliferation in vitro (IC50=2.4nM). In MDA-MB-436 tumor cells, XZP-7797 inhibits PARylation and increases γH2AX level in a concentration-dependent manner. XZP-7797 demonstrates rapid tumor regression in a dose-dependent manner in the BRCA1 mutant MDA-MB-436 breast cancer xenograft model. In a pancreatic cancer xenograft model, XZP-7797 can also significantly inhibit tumor growth. To evaluate the ability of XZP-7797 to cross the blood-brain barrier, an intracranial xenograft model of breast cancer is used. The results show that XZP-7797 can significantly inhibit the growth of intracranial tumors in mice at a dose of 10 mg/kg, demonstrating the brain-penetrating ability of XZP-7797. Additionally, XZP-7797 has great PK profiles in preclinical species after oral dosing and good ADME properties. In a 14-day toxicology study of XZP-7797 in SD Rats, XZP-7797 has no hematological adverse effects at the exposure that is 10 times higher than the exposure required to achieve superior efficacy.
Citation Format: Yeqing Gao, Yuqian Lu, Bo Chen, Benke Jiang, Feng Wang, Genyan Zhang, Xifeng Ma, Dapeng Zhou, Chunmin Shi, Yunling Wang, Zhipeng Liu, Jun Sun, Huimin Zhou, Mei Xu, Jiakui Li, Bin Liu. Discovery of a potent, selective and brain penetrating PARP1 inhibitor, XZP-7797 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB268.
Abstract LB286: Extrachromosomal DNA in lung cancer from never smokers
Authors: Khandekar Azhar , Zhang Tongwu , Diaz-Gay Marcos , Luebeck Jens , Alexandrov Ludmil , Landi Maria 
Published: April 2024

Abstract
Extrachromosomal DNA (ecDNA), circular DNA that resides outside of the linear chromosome, is a potent form of oncogene amplification in cancer. The structure and properties of ecDNA are markedly different from those of linear DNA, characterized by features like non-Mendelian inheritance and an altered epigenetic landscape. A prior pan-cancer analysis ​​indicated patients with cancers harboring ecDNA have statistically worse overall survival when compared to cancer patients with other types of focal amplification. Moreover, its unique characteristics also make it a promising target for therapy. Recently, efforts have intensified to profile ecDNA across many cancer types, yet comprehensive studies of ecDNA in lung cancer in never smokers (LCINS) is lacking. Given that LCINS is partially driven by somatic copy number alterations, we hypothesized that ecDNA-driven focal amplifications significantly influence LCINS tumor evolution. As part of the Sherlock-Lung project (current data freeze, 1217 samples), we comprehensively profiled 871 treatment-naive samples using a robust computational pipeline that refined copy number segmentation by accounting for tumor purity and ploidy. ecDNA was present in 151 (17.3%) of tumor samples, with a third of ecDNA-positive samples harboring more than one distinct ecDNA amplicon. Consistent with prior reports, no ecDNA was found in bulk sequenced blood or matched normal lung tissues. These ecDNA often included genes (84%), frequently overlapping with known oncogenes like MDM2, TERT, and MYC, which showed higher expression levels compared to non-amplified or other amplified genes. Notably, most MDM2 amplifications (36/45) were on ecDNA. ecDNA was significantly associated with whole genome doubling, tumor stage, and several endogenous mutational signatures, including APOBEC (SBS2 and SBS13), and clock-like signature (SBS1). Intriguingly, ecDNA-positive tumors were associated with significantly worse survival compared to tumors without any focal amplifications, though tumors with other amplification types (e.g. breakage-fusion-bridge, linear amplification, or complex events) had similar survival impacts. The unprecedented size of our cohort and the integration of clinical, epidemiologic, and multi-omics data allowed us to perform the first in-depth characterization of ecDNA in LCINS. We reconstructed the genomic regions involved in ecDNA and revealed the full spectrum of simple and complex ecDNAs across cancer histologies as well as the clinical and functional impact of ecDNA on LCINS.
Citation Format: Azhar Khandekar, Tongwu Zhang, Marcos Diaz-Gay, Jens Luebeck, Ludmil Alexandrov, Maria Landi. Extrachromosomal DNA in lung cancer from never smokers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB286.
Abstract LB292: Glutathione supports lipid abundance in vivo
Authors: Asantewaa Gloria , Tuttle Emily T. , Ward Nathan P. , Kang Yun P. , Kim Yumi , Kavanagh Madeline , Huber Aaron R. , Munger Joshua , Cravatt Benjamin , Cole Calvin L. , DeNicola Gina M. , Harris Isaac S. 
Published: April 2024

Abstract
Glutathione (GSH), the most abundant antioxidant, scavenges free radicals and prevents the detrimental effects of oxidative stress. As a result, GSH levels are associated with protection from stress-related conditions, such as aging and cancer. Given this rationale, antioxidants are routinely consumed by the public, mainly because they are viewed as “cure-alls” and almost uniformly beneficial to a range of diseases. This is an oversimplification, as the relationship between antioxidants and diseases is much more complex. Recent evidence has implicated GSH in protecting cancer cells from oxidative stress and promoting their survival. Therefore, understanding GSH metabolism in vivo and its intricate crosstalk with other cellular pathways is vital to improving the efficacy of therapies for diseases, including cancer. To interrogate this, we developed a series of in vivo models to induce Gclc deletion in adult animals. We find that GSH is essential to lipid abundance in vivo. GSH levels are reported to be highest in liver tissue, which is also a hub for lipid production. While the loss of GSH did not cause liver failure, it decreased lipogenic enzyme expression, circulating triglyceride levels, and fat stores. Mechanistically, we found that GSH promotes lipid abundance via the LXR/SREBP signaling pathway, as well as by repressing NRF2, a transcription factor induced by oxidative stress. Overall, these findings suggest an essential function for GSH in maintaining circulating lipids levels and lipid depots, possibly as a mechanism to prevent lipid peroxidation and redox imbalances. Notably, the accumulation of oxidized lipids propagates oxidative stress and associated cell death phenotypes (i.e., ferroptosis). This also highlights a potentially novel mechanism by which GSH regulates lipid production and suggests a possible link between oxidative stress (caused by GSH deficiency) and cachexia (commonly characterized by loss of fat and lean mass), a disorder linked to cancer but poorly understood. Consequently, by elucidating the contributions of GSH to lipid homeostasis, we can better understand the basic biology surrounding GSH and obtain critical insight into potentially improving the treatment of cancer-related cachexia.
Citation Format: Gloria Asantewaa, Emily T. Tuttle, Nathan P. Ward, Yun P. Kang, Yumi Kim, Madeline Kavanagh, Aaron R. Huber, Joshua Munger, Benjamin Cravatt, Calvin L. Cole, Gina M. DeNicola, Isaac S. Harris. Glutathione supports lipid abundance in vivo [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB292.
Abstract LB318: Exploring early precursor lesions of ovarian cancer: Insights from an in vitro model
Authors: Wang Yan E. , Levina Vera , Galson Deborah L. , Lokshin Anna E. 
Published: April 2024

Abstract
Ovarian cancer ranks as the fifth leading cause of cancer-related deaths in women, surpassing other cancers of the female reproductive system. Recent epidemiological and molecular studies highlight the significance of high-grade serous carcinoma (HGSC), the most aggressive subtype of ovarian cancer, constituting nearly 96% of cases with mutations in p53. HGSC originates from fallopian tube secretory epithelial (FTSEC) cells through a progression of precursor lesions, ranging from p53 signatures to serous tubal intraepithelial carcinoma (STIC). The p53 signature involves a linear expansion of over 12 TP53 mutant FTSECs, morphologically normal and displaying no increase in proliferative activity. In this study, we established a model system for P53 signature by either overexpressing p53 mutations (R175H and R273H) or knocking down P53 expression in normal FTSECs maintained as 2D, 3D, or organoid cultures. We have characterized the cells using RNA seq, mass spectroscopy and functional assays. Functional assays revealed increased migration and proliferation, along with decreased apoptosis in response to the cytotoxic stimulus (cisplatin). Despite the inability of p53 signature cells to proliferate or migrate in vivo, our hypothesis posited that clonal expansion of progenitor cell with a p53 mutation maintains plasticity by preserving the stemness of one or more cells capable of responding to cues from the microenvironment. Our experiments revealed heterogeneity in the model system, reflecting the composition of p53 signature in vivo. We also observed that stem-like cells tend to organize into clusters. Notably, stem-like cluster cells bearing a p53 signature exhibit migratory behavior, whereas clusters lacking p53 do not demonstrate such mobility. Given that most high-grade serous carcinoma (HGSC) cases occur in postmenopausal women, we demonstrated induction of proliferation and migration of p53 mutant stem cells in response to stimuli from aging stromal cells (fibroblasts and autologous immune cells) and to changes in hormonal background in our model. Conclusion: This study illuminates a novel aspect of ovarian cancer development, underscoring the role of a distinct population of fallopian tube epithelial cells with stem-like properties within p53 signatures. Further investigations into the tumorigenicity of these cells hold promise for providing valuable insights into future therapeutic strategies.
Citation Format: Yan E. Wang, Vera Levina, Deborah L. Galson, Anna E. Lokshin. Exploring early precursor lesions of ovarian cancer: Insights from an in vitro model [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB318.
Abstract LB320: Runx2 as a key transcription factor regulating contractility and extracellular matrix remodeling in abnormally activated fibroblasts: Implications for tumorigenesis and keloid pathogenesis
Authors: Lee Keun-Woo , Sohn Insuk , Kim Seok-Hyung , Yeo So-Young 
Published: April 2024

Abstract
Cancer associated fibroblasts (CAFs) express a variety of cytokines and factors that activate and contribute to pathways that favor tumorigenesis. CAFs disrupt normal cellular functions, such as cell cycle regulation and cell death including apoptosis, or signal certain types of cells to abnormally activate pro-tumor activities. In addition, when it is abnormally activated, it is characterized by increased collagen deposition and more contractility. Likewise, This pattern of abnormal activation is often observed in keloid disease. Keloid constitutes an abnormal fibroproliferative wound healing response with excessive collagen deposition causing abnormal wound healing. To better understand the CAF subtypes with abnormal activation and cancer-promoting properties and the subtypes of keloid fibroblasts with similar activation properties, it is essential to find the master transcription factors (TFs) that determine their identity. To identify functionally important TFs in abnormal CAFs and keloid fibroblasts, we analyzed H3K27ac ChIP-seq of CAFs and keloid fibroblasts and observed that Runx2 significantly enriched the two types of abnormal fibroblasts.To investigate the biological function of Runx2 , we established CAFs from colon cancer patients and performed ShRNA-mediated knockdown of the RUNX2 gene. We confirmed that knockdown of RUNX2 in CAFs suppressed the migration or invasion of cancer cells. Additionally, using a gel contraction assay, we confirmed that fibroblast contractility was significantly reduced by RUNX2 knockdown. Similarly, in keloid fibroblasts, it was confirmed that the migration and gel contraception abilities of fibroblasts were significantly reduced following knockdown of RUNX2 [A1] gene. Last but not least, expression of ECM elements, such as collagens and fibronectin, were significantly as a result of the knockdown of the RUNX2 gene in both abnormally activated fibroblasts. To conclude, we believe that Runx2 acts as a key TF that maintains the contractility and ECM remodeling ability of abnormally activated fibroblasts. Our findings indicate that Runx2 is a attractive therapeutic target not only for pro-tumorigenesis in the cancer microenvironment, but also for keloid fibroblasts.
Citation Format: Keun-Woo Lee, Insuk Sohn, Seok-Hyung Kim, So-Young Yeo. Runx2 as a key transcription factor regulating contractility and extracellular matrix remodeling in abnormally activated fibroblasts: Implications for tumorigenesis and keloid pathogenesis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB320.
Abstract LB342: Potent Anti-Tumor Activity Demonstrated by Anti-AXL CAR T Cell Therapy and AXL-ADC in Lung Cancer and Breast Cancer Models
Authors: Wu Zhongen , Gao Yang , Pei Jingpeng , Zhao Enfeng , Mei Shenglin , Feng Yuxiong , Zhu Di 
Published: April 2024

Abstract
AXL is over-expressed in various cancers. Its signaling pathway is related to tumor progress. We created a novel a novel AXL-ADC (Hu002-MMAE) based on humanized AXL-targeted antibody (Hu002). We also developed anti-AXL CAR T cells and AXL-CAR iNKT to evaluate anti-tumor effect. Methods: 1) Evaluate the cytotoxicity of Hu002-MMAE in 5 AXL-high-expressing cells. 2) in vivo efficacy validation of Hu002-MMAE into LCLC-103H-bearing Nude mice and U87MG-bearing nude mice. 3) Evaluate the cytotoxicity of anti-AXL CAR-T and AXL CAR-iNKT cells in cell lines with different AXL expression levels to validate the selectivity. 4) in vivo efficacy validation of anti-AXL CAR T cells into MDA-MB-231-bearing mice.Results: Hu002 demonstrates potent binding affinity significantly inhibited the migration of AXL-high tumor cells. The Hu002-MMAE showed potent cytotoxicity against AXL-high tumor cells (IC50 &lt; 0.1 nmol/L) and had a longer-term therapeutic effect in a variety of NSCLC and glioma tumors in vivo (MED &lt; 0.5 mg/kg). Anti-AXL CAR-T cells and AXL CAR-iNKT exhibited strong killing activity to AXL-high-expressed cell lines LCLC-103H and MDA-MB-231 in vitro, while shows minimum effect of anti-tumor effect in AXL-low-expressed cell lines H460 and MDA-MB-453. In in vivo models of MDA-MB-231, we observed increased infiltration of T cells in primary tumor and reduced lung metastasis after AXL CAR-T treamtment.Conclusion: Anti-AXL CAR T and AXL-ADC cells showed strong and selective killing activity, improved TME in primary tumor and mitigate lung metastasis, which makes it a potential strategy for cancers.
Citation Format: Zhongen Wu, Yang Gao, Jingpeng Pei, Enfeng Zhao, Shenglin Mei, Yuxiong Feng, Di Zhu. Potent Anti-Tumor Activity Demonstrated by Anti-AXL CAR T Cell Therapy and AXL-ADC in Lung Cancer and Breast Cancer Models [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB342.