bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing and Testing a Cooling System for CMOS in DSLR to Minimize Noise in Astronomical Image and Incorporating a Sky Quality Meter
Authors: Syahputra Hanif Nanda , Nuryanto Fadhil Muhammad , Iskak Farid Abyan Maulana 
Published: April 2024

Abstract
To capture faint astronomical images effectively, commercial camera like Digital Single-lens reflects (DSLR) require operation at low temperatures to minimize dark current noise. To achieve this, a cooling system utilizing Thermoelectric Cooler (TEC) technology is devised. This cooling apparatus is regulated by a microcontroller employing digital PID control. The design process for the cooler and its fan is conducted via computational fluid dynamics (CFD) simulations. Real-time temperature data is acquired using a temperature sensor affixed to the rear of the camera sensor. This data serves as input for the digital PID control algorithm, with the calculated PID results then applied to the PWM controller linked to the TEC. Consequently, the camera sensor temperature is effectively lowered in accordance with the specified target temperature. Apart from that, a sky quality meter is also installed on the device to measure the brightness of the night sky. By integrating the sky quality meter with the DSLR, researchers can obtain additional data in the form of sky brightness, which will be very useful in analyzing and interpreting astronomical images. In this paper, a DSLR camera cooling system integrated with a sky quality meter will be designed, tested and discussed in detail.
Evaluation of four growth mediums effect on potato (Solanum tubérosum L.) plantlet production in Burkina Faso
Authors: ZERBO Afoussatou , SOME Koussao , TIAMA Djakaridja , SORO Monique , OUEDRAOGO Wendpang-yidé Idrissa Caleb , TRAORE Renan Ernest 
Published: April 2024

Abstract

In Burkina Faso, potato growers are faced with difficulties in obtaining seed. These seeds, mostly imported partially meet the quality requirement in potato production. Furthermore, these seeds are not accessible in term of cost and also not available in time. Local seed production is therefore necessary. In vitro culture can remedy this problem by providing quality seeds. Thus, the goal of our study was to set up an in vitro culture protocol for the production of plantlets with optimal vegetative growth. This study was realised in the in vitro culture laboratory at INERA Kamboinsé. Explants from in vitro plantlets (1 cm) of the same generation were used as plant material and transplanted onto four different mediums. The mediums used were: M0= 4,4g/l MS medium (Murashige and Skoog 1962) + 7g/l agar; M1= M0 + 30g/l table sugar; M2= M0 + 0,5mg/l AIB and M3= M0+30g/l sugar + 0,5mg/l AIB. These mediums were prepared with a pH=5,7±0.1, then divided into jars and autoclaved for 20 minutes at a pressure of 120 bars. Subculture in the jars was realised out in a laminar flow hood. The covered jars were transferred to a growth room for 30 days. The results of this study indicated that M1 and M3 showed good regeneration of the in vitro plantlets compared to M0 and M2. Sugar probably had a positive effect on root length, stem diameter, number of nodes and number of leaves open. These parameters strongly differentiated M1 from the other mediums. AIB had a positive effect on the root proliferation of in vitro plantlets in the M2 medium. The combined effect of sugar and AIB was even more positive on stem height, number of roots, number of leaves open and weight of in vitro plantlets, which strongly differentiated medium M3 from the other mediums. In conclusion, M3 proved to be the best medium for producing in vitro plantlets with good vegetative growth for seed production.
An investigation of perinatal mental health needs: A qualitative content analysis
Authors: Dehghan Fateme , Kolivand Mitra , Sadeghi Mohammad , Sooki Zahra 
Published: April 2024

Abstract

Background and aims: Perinatal psychological problems significantly affect the health of mothers and infants. Assessment of mental health needs during pregnancy can help improve strategies and planning for mothers' mental health. Therefore, the present study aimed to explore perinatal mental health needs.
Methods: The present qualitative research was conducted in Kermanshah city in 2022. The participants were 11 pregnant women selected purposively with maximum variety, and took part in semi-structured interviews. The data collection continued until data saturation. The data were analyzed through a conventional content analysis, simultaneously with the sampling and data collection. Data management was performed in MAXQDA.
Results: The data analysis led to the extraction of five themes: Mental health care (6 categories), Supportive communications (7 categories), Knowledge and skill (3 categories), socioeconomic welfare (5 categories) and professional services (6 categories).
Conclusion: The present study found five aspects of needs concerning pregnant mothers’ mental health. The husband, family, society, specialist doctor and midwives can play important roles in satisfying these needs.
Evaluation and limitations of the novel chemiluminescent enzyme immunoassay technique for measuring total tau protein in the cerebrospinal fluid in patients with human prion disease: a 10-year prospective study (2011–2020)
Authors: Weijie Kong , Nonaka Toshiaki , Satoh Katsuya 
Published: April 2024

Abstract

Background: Recently, the investigation of cerebrospinal fluid (CSF) biomarkers for diagnosing human prion diseases (HPD) has garnered significant attention. Reproducibility and accuracy are paramount in biomarker research, particularly in the measurement of total tau (t-tau) protein, which is a crucial diagnostic marker. Given the global impact of the coronavirus disease pandemic, the frequency of measuring this protein using one of the world's fully automated assays, chemiluminescent enzyme immunoassay (CLEA), has increased. At present, the diagnosis and monitoring of neurological diseases mainly rely on traditional methods, but their accuracy and responsiveness are limited.there is a limited knowledge on the accuracy of CLEA in Tau measurements. We aimed to measure t-tau protein using CLEA and to elucidate its merits and limitations.
Methods: We analysed CSF samples obtained from 91 patients with rapidly progressive dementia using ELISA and CLEA. Additionally, we used western blotting to detect the presence of 14-3-3 protein and employed real-time quaking-induced conversion (RT-QuIC) assays to analyse the same set of samples. Furthermore, we examined the correlation coefficient between ELISA and CLEA results in a subset of 30 samples. Moreover, using CLEA, we evaluated the diurnal reproducibility, storage stability, dilutability, and freeze-thaw effects in three selected samples.
Results:Among the 91 patients, a total of 45 (22 men and 23 women) tested positive for HPD in the RT-QuIC assay. In contrast, all CSF samples from the remaining 46 patients without HPD (23 men and 23 women) tested negative in the RT-QuIC assay. Both ELISA and CLEA showed perfect sensitivity and specificity (100%) in measuring t-tau protein levels. Furthermore, there was a strong correlation coefficient (R² = 0.9363) between ELISA and CLEA results. However, despite its advantages, CLEA analysis exhibited instability for certain samples with t-tau protein levels exceeding 2,000 pg/mL, leading to low reproducibility during dilution analysis.
Conclusions: Our findings indicate that CLEA outperforms ELISA in terms of diurnal reproducibility, storage stability, and freeze-thaw effects. However, ELISA demonstrated superior performance in the dilution assay. Therefore, it is imperative to develop innovative approaches for the dilution of biomarker samples for CLEA measurements during clinical trials.
Investigation of potential detrimental effects of nano- and microplastics in human endometrial stromal cells
Authors: Kim Nara , Park Joo Hyun , Jung Gee Soo , Lee Inha , Lee Jae Hoon , Lee Min Jung , Im Wooseok , Cho SiHyun , Choi Young Sik 
Published: April 2024

Abstract

Nanoplastics (NPs) and Microplastics (MPs) have become a global concern in recent years. However, the effects of NPs and MPs on the female reproductive system have not been elucidated, yet. In this study, we aimed to establish proper in vitro NPs and MPs exposure protocol and to investigate possible cytotoxic effects of NPs and MPs on human endometrial cells, using different plastic size and concentrations. It was noted that smaller plastics, specifically 100 nm NPs and 1 µm MPs, exhibited a higher propensity for cellular uptake compared to larger counterparts such as 5 µm MPs. These smaller plastic particles demonstrated the ability to induce notable morphological changes and provoke cell death at concentrations exceeding 100 µg/mL over a 24-hour period. Furthermore, we confirmed the accumulations of these particles in the nucleus and cytoplasm of endometrial cells using confocal microscopy and a real-time imaging device. A significantly higher rate of internalization was observed, manifesting a discernible correlation between the size of the plastics and the degree of penetration into both the nucleus and cytoplasm. In conclusion, this is the first study to demonstrate that exposures of 100 nm NPs and 1 µm MPs results to dynamic accumulations of these particles within endometrial stromal cells, leading to cell death at specific concentrations. Our findings imply potential adverse impacts of these particles on fertility and reproductive health, underscoring the need for further research to elucidate the exact underlying molecular mechanisms.
Does living longer mean living healthier? A comprehensive analysis of 204 countries and regions from 1990 to 2019
Authors: Xiong Bo , Chen Yuxiao , Chen Ning 
Published: April 2024

Abstract

Background The data from the WHO showed that life expectancy and health-adjusted life expectancy at global level both increased from 2000 to 2019, while they did not increase at the same rate. Therefore, our study aims to explore the relationship and changing trends between life expectancy and health-adjusted life expectancy, and further comprehensively analyze the factors influencing the difference between the two. Methods This paper uses data from the Global Burden of Disease (GBD) database in life expectancy and health-adjusted life expectancy across 204 countries and regions from 1990 to 2019. It analyses the trends of the relationship between life expectancy and health-adjusted life expectancy at birth and at 65 and over across 204 countries and regions from 1990 to 2019 and classfies all the coutries into three typies: optimized countries, low deterioration countries and high deterioration countries. Then this paper uses the random effects model to analyze the factors that influence the three types of countries and regions in terms of environmental/occupational risk, behavioral risk, and metabolic risk. Results First, for males, for indicator of age at birth, 8 optimized countries were with "drug use" having the greatest impact, 98 low deterioration countries were with "low physical activity" being the most significant; 98 high deterioration countries were with "childhood sexual abuse and bullying" being the most significant; for indicator of age at 65 and over, 18 optimized countries were with "low physical activity" having the greatest impact, 98 low deterioration countries were with "drug use" being the most significant; 88 high deterioration countries were with "occupational risk" being the most significant. Second, for females, for indicator of age at birth, 6 optimized countries were with "drug use" having the greatest impact, 107 low deterioration countries were with "child and maternal malnutrition" being the most significant; 91 high deterioration countries were with "drug use" being the most significant; for indicator of age at 65 and over, 14 optimized countries were with "occupational risks" having the greatest impact, 109 low deterioration countries were with "occupational risk" being the most significant; 81 high deterioration countries were with "drug use" being the most significant. Conclusions Based on the results, it suggested that the policy need to focus on the facts of drug use, occupational risk and low physical activity to narrow the gap between life expectancy and health life expectancy.
Assessing urban riverscapes: A multiscale approach designed for management application
Authors: Murphy Brian M. , Nelson Peter A. , Powell Mary , Gilbert Josh 
Published: April 2024

Abstract

Urban riverscapes are integral to the livability and sustainability of cities and suburbs. Conserving and restoring these riverscapes requires assessing their condition over a broad range of spatial scales, from watershed to planning segment to reach scale. Any riverscape assessment must examine the hydrogeomorphic and ecological characteristics and behaviors in order to provide decision-making information to resource managers. The novel assessment method introduced in this article, Urban Riverscape conditions-Based Assessment for management Needs (URBAN), provides a framework to characterize the physical condition of and the social-ecological values within a watershed, as well as the anthropogenic impacts affecting those functions and values, that can inform restoration planning and riverscape management. URBAN is founded upon the third tier of the Urban Riverscapes Assessment Framework – “How should we assess the values and functions of an urban riverscape?” Assessment of physical conditions and characteristics in urban riverscapes using the URBAN indicators and metrics provides critical information about the underlying watershed system status and stream evolution trajectory. URBAN includes both data collection and the evaluation of desktop and field data to determine stream characteristics. Data collection emphasizes reach-typing (or stream classification) and related or relevant aspects of stream physical condition. URBAN is unique in its emphasis upon stream “values,” which links the physical processes of a stream and its social-ecological values to anthropogenic stressors that may influence the long-term recovery of degraded streams. These linkages also provide critical information for planning instruments, regulatory requirements, and community conversations. Coupled with GIS tools, urban riverscape studies at multiple scales, like URBAN, provide objective methods to understand and interpret multi-causal mechanisms and trends that influence the physical condition and potential recovery of degraded urban riverscapes. This article provides a summary of those methods and the tools applied by way of remote and rapid field assessments, through the lens of an urban watershed assessment conducted in the Colorado Front Range. The outcome of this work advances the scientific basis underlying applied riverscape management and broad-scale preservation and renovation planning.
Protocol for the Development of a Tool to Map Systemic Sclerosis Pain Sources, Patterns, and Management Experiences: A Scleroderma Patient-Centered Intervention Network Patient-Researcher Partnership
Authors: Santo Tiffany Dal , Golberg Meira , Nassar Elsa-Lynn , Carrier Marie-Eve , Hu Sophie , Kwakkenbos Linda , Bartlett Susan J. , Fox Rina S. , Lee Yvonne C. , Varga John , Benedetti Andrea , Thombs Brett D 
Published: April 2024

Abstract
Introduction: Systemic sclerosis (SSc) is a rare, complex autoimmune rheumatic disease with multiple factors that contribute to pain. People with SSc emphasize the effect pain has on their quality of life, but no studies have systematically examined the frequency and relative importance of different SSc pain sources, patterns of pain from different sources, and pain management experiences. Our objectives are to (1) develop a tool, jointly with researchers, health care providers, and patients, to map sources of pain in SSc, determine patterns of pain from different sources, and understand pain management experiences; and (2) administer the final tool version to participants in the large multinational Scleroderma Patient-centered Intervention Network (SPIN) Cohort.
Methods: First, we will use validated pain assessment tools as templates to develop an initial version of our pain assessment tool, and we will obtain input from patient advisors to adapt it for SSc. The tool will include questions on pain sources, pain patterns, pain intensity, pain management techniques, and barriers to pain management in SSc. Second, we will conduct nominal group technique sessions with people living with SSc and health care providers who care for people with SSc to further refine the tool. Third, we will conduct individual usability testing sessions with SPIN Cohort participants. Once the tool has been finalized, we will administer it to individuals in the multinational SPIN Cohort, which currently includes over 1,300 active participants from 54 sites in 7 countries. We will perform unsupervised clustering using the KAy-Means for MIxed LArge data (KAMILA) method to identify participant subgroups with similar profiles of pain sources (present or absent) and to evaluate predictors of subgroup membership. We will use latent profile analysis to identify subgroups of participants with similar profiles based on pain intensity scores for each pain source and evaluate predictors.
Discussion: Once completed, our pain assessment tool will allow our team and other researchers to map sources of pain in SSc and to understand pain management experiences of people living with SSc. This knowledge will provide avenues for studies on the pathophysiology of pain in SSc and studies of interventions to improve pain management.
Genetic diversity and comparative genomics across Leishmania (Viannia) species
Authors: Duitama Jorge , Gonzalez-Garcia Laura , Rodriguez Maria , Parra-Muñoz Marcela , Clavijo Ana , Levy Laura , Ovalle-Bracho Clemencia , Colorado Claudia , Camargo Carolina , Quiceno Eyson , Moncada Maria , Muskus Carlos , Urrea Daniel , Baez-Aguirre Felipe , Restrepo Silvia , Echeverry Maria 
Published: April 2024

Abstract
Leishmaniasis is a disease representing an important public health problem worldwide, with a broad spectrum of clinical and epidemiological features partly associated with the diversity and complex life cycle of the Leishmania parasites. This study analyzes genomic data from 205 Leishmania (Viannia) samples, including 66 newly sequenced clinical isolates. It also provides chromosome-level genome assemblies for 10 isolates representing different species and populations. The observed distribution of Leishmania genomic diversity across the sampling locations suggests rapid adaptation to different ecosystems. Pangenomic analysis of high-quality assemblies shows consistent copy number variation between species for different gene families. Amastin gene families have larger numbers and diversity than previous reports based on analysis of short-read data. This work provides comprehensive genomic resources to identify population markers for Leishmania spp, leveraging valuable insights into the biology, transmission dynamics, the evolution of virulence mechanisms, and the spread of resistance of the parasite.
WITHDRAWN: Research on pruning algorithm of the ViT model based on local linear embedding method
Authors: Wang Wei , Liu XiaoRui , You KeXin 
Published: April 2024

Abstract
To realize intelligent driving of new energy vehicles, traffic sign recognition has high requirements on storage and computing resources, increasing the cost of vehicle hardware. To solve this problem, the Vision Transformer (ViT) model is applied to classify traffic signs. However, due to its large number of parameters and the huge computation amount, the ViT model is difficult to be widely used in resource-limited platforms. This paper presents a similarity filter pruning method based on locally linear embedding. By using the alternating direction multiplier method and the loss of the locally linear embedding method for the model training function, the proposed pruning method prunes the operation model mainly by evaluating the similarity of each layer in the network layer filters. According to the pre-set pruning threshold value, similar filters to be pruned are obtained, and the filter with a large cross-entropy value is retained. The results on the BelgiumTS and GTSRB datasets indicate that the proposed SJ-LLE pruning algorithm can reduce the number of parameters of the multi-head self-attention module and Multi-layer Perceptron (MLP) module of the ViT model by more than 60%, and the loss of model accuracy is acceptable. The scale of the ViT model is greatly reduced, which is conducive to the application of this model in embedded traffic sign recognition equipment. Also, this paper proves the hypothesis through experiments that "using the LLE algo-rithm as the loss function for model training before pruning plays a positive role in reducing the loss of model performance in the pruning process".
Heart Failure Attributable to Hemoglobinopathies and Hemolytic Anemia: A Three-Decade Cross-Sectional Assessment of the Global Burden
Authors: Deng Xiaoqi , Yu Lei , He Jie , Fu Yufan , Jiao Lan 
Published: April 2024

Abstract

Background
Complexity of anemia subtypes remains unresolved, and therapies targeting anemia have inconsistently improved heart failure (HF) outcomes. This study aims to assess the prevalence trend and contributing factors of HF impairment with hemoglobinopathies and hemolytic anemia at global, regional and national levels.
Main body of the abstract:
Utilizing Global Burden of Disease (GBD) data for HF and hemoglobinopathies inclusive of hemolytic anemia, we systematically gathered annual figures for prevalence and incidence. Estimated Annual Percentage Changes (EAPCs) were computed to assess temporal trends in these diseases. Estimates were subsequently disaggregated by sex, geographical regions, and national levels to present a concise yet detailed picture of the disease dynamics globally. During the past three decades, although the absolute caseloads of hemoglobinopathies and hemolytic anemias grew without altering their standardized prevalence (EAPC = 0.26), the rate of heart failure compounded by anemia sharply rose (EAPC = 0.49). Notably, in high Sociodemographic Index (SDI) regions, the HF-to-hematological disorder ratio ascended more rapidly, moving from 82.80 parts per million (ppm) in 1990 to 114.22 ppm in 2019, surpassing the worldwide average increment (40 ppm). Despite greater anemia-related burdens among females, male patients experienced a disproportionately higher frequency of heart failure.
Short conclusion:
Over the past three decades, there has been a steady rise in the prevalence of heart failure comorbid with hemoglobinopathies and hemolytic anemias, with a more pronounced disease burden observed among men and a discernible shift toward High SDI regions.