The mei‐9a test for chromosome loss in drosophila: A review of assays of 21 chemicals for chromosome breakage
Authors: Zimmering S. 
Published: February 2007

AbstractIn Drosophila, detection of chemical‐induced chromosome breakage for many compounds in conventional chromosome loss or reciprocal translocation (RT) tests requires considerably higher concentrations than for sex‐linked recessive lethals, or fails entirely even at concentrations giving substantial to high rates of recessive lethals. Accordingly, relatively high LDs may be necessary before evidence of chromosome breakage is observed raising questions of “false positives” resulting, for example, from aberrant metabolic effects/products not found at lower concentrations.In the test for chromosome loss, definitive scoring is made in F1 progeny. In contrast, an F2 and often an F3 is required to bring the RT test to completion. Further, the RT test is prone to “false negatives” in many cases unless testing is carried out on sperm stored in the female for several days (or longer). Accordingly, efforts in this laboratory have been directed toward improvements in the sensitivity of the test for chromosome loss. Major advances have been made by using excision repair deficient mei‐9a females as PI females to which treated males are mated.For purposes of testing, ring‐X males carrying the doubly marked Y chromosome, BsYy+, were treated or not, mated for 3 days with ordinary (repairproficient) females (the conventional test) or with mei‐9a females (the mei‐9a test) and all PIS discarded. Data are reported for this period only‐viz, on unstored sperm. F1 progeny were scored for complete loss (CL) of the X or Y (loss is principally of the ring‐X) and partial loss of the Y (PL)‐viz, loss of Bs or y+. Partial loss of the Y chromosome was taken as strong evidence of chromosome breakage, whereas loss of the ring‐X was regarded alternatively as break‐related or resulting from the production of sister chromatid exchanges.Of the 21 compounds tested, 17 are classified as carcinogens and four are unknown in this regard. At the concentration reported, 21/21 compounds were positive for CL+PL and 21/21 for PL in the mei‐9a test. At the same concentration, only 10/20 compounds were positive for CL and PL and 2/21 compounds for PL with repair proficient females; the mei‐9a test is clearly more sensitive than the conventional test for chromosome breakage. Data were compared with results from RT tests primarily from the work of others where the concentration was no lower or the duration of treatment less than that in the mei‐9a experiments. 14/21 compounds were judged positive and seven negative in the RT test; four of the negatives are classified as carcinogens. Most positives required sperm storage.Accordingly, the mei‐9a test for chromosome loss may be regarded as a far more rapid and sensitive test for chromosome breakage than the conventional reciprocal translocation test for the compounds tested (1) in light of the experimental results indicated above; (2) the mei‐9a test requires only an F1 for completion; (3) 21/21 chemicals were positive for chromosome breakage in unstored sperm in the mei‐9a test, and (4) with the exception of two experiments where LD values were 8 and 15, respectively, LD values were in the range 0–5.