bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protamine usage following implantation of drug‐eluting stents: A word of caution
Authors: Cosgrave John , Qasim Asif , Latib Azeem , Aranzulla Tiziana C. , Colombo Antonio 
Published: March 2008

AbstractThe treatment of hemorrhagic complications of percutaneous interventions with protamine is an accepted practice. Concerns exist about the increased thrombo‐genicity of drugeluting stents. We report two cases of acute stent thrombosis following drug‐eluting stent implantation related to Protamine administration. © 2008 Wiley‐Liss, Inc.
Difficult stent delivery: Use of an aspiration catheter as a “sheath”
Authors: Ashikaga Takashi , Nishizaki Mitsuhiro , Yamawake Noriyoshi 
Published: March 2008

AbstractThis is a report of a case in which stent delivery to the lesion location proved difficult because of severely calcified and tortuous proximal vasculature. Efforts to deliver a stent using a buddy wire, anchoring technique and five‐in‐seven guiding system were unsuccessful, whereas an aspiration catheter enabled stent delivery to the target lesion. This technique offers a potential alternative for cases in which some stent delivery methods are problematic. In addition, this technique may also prevent potential injury to drug‐eluting polymer during stent delivery. © 2008 Wiley‐Liss, Inc.
Dorsalis pedis artery stenting for limb salvage
Authors: Kawarada Osami , Yokoi Yoshiaki 
Published: March 2008

AbstractStent‐supported angioplasty has emerged as an effective alternative to distal bypass for infrapopliteal artery disease. This treatment has been mainly employed in the tibial and peroneal arteries. However, the pedal artery below the ankle is the final frontier for catheter intervention. Here, we describe the utility of dorsalis pedis artery stenting for limb salvage. © 2008 Wiley‐Liss, Inc.
Will Genetic Profiling Make a Difference in Clinical Trial Outcomes?
Authors: Conti C. Richard 
Published: March 2008

AbstractWhat stimulated me to write this editorial was an article I read in USA Today written by Lois Hatton, who is a writer and columnist based in Brookings, South Dakota and author of “Inspiration for a Lifetime.” The article, “‘Black’ Cherokees fight for heritage,” told of a black Yankton Sioux who says he is seen as a black by other Sioux while on the reservation, but as an Indian by blacks who do not live on the reservation. Copyright © 2008 Wiley Periodicals, Inc.
Expression of avian prickle genes during early development and organogenesis
Authors: Cooper Oliver , Sweetman Dylan , Wagstaff Laura , Münsterberg Andrea 
Published: March 2008

AbstractChicken homologues of prickle‐1 (pk‐1) and prickle‐2 (pk‐2) were isolated to gain insight into the extent of planar cell polarity signaling during avian embryogenesis. Bioinformatics analyses demonstrated homology and showed that pk‐1 and pk‐2 exhibited conserved synteny with ADAMTS20 and ADAMTS9, GON‐related zinc metalloproteases. Expression of pk‐1 and pk‐2 was established during embryogenesis and early organogenesis, using in situ hybridization and sections of chicken embryos. At early stages, pk‐1 was expressed in Hensen's node, primitive streak, ventral neural tube, and foregut. In older embryos, pk‐1 transcripts were detected in dorsolateral epithelial somites, dorsomedial lip of dermomyotomes, and differentiating myotomes. Furthermore, pk‐1 expression was seen in lateral body folds, limb buds, and ventral metencephalon. pk‐2 was expressed in Hensen's node and neural ectoderm at early stages. In older embryos, pk‐2 expression was restricted to ventromedial epithelial somites, except in the most recently formed somite pair, and limb bud mesenchyme. Developmental Dynamics 237:1442‐1448, 2008. © 2008 Wiley‐Liss, Inc.
Summary of ‘Bicycle helmet legislation for the uptake of helmet use and prevention of head injuries’
Authors: Milne Andrea 
Published: March 2008

AbstractThis is a summary of a Cochrane review, published in this issue of EBCH, first published as: Macpherson A, Spinks A. Bicycle helmet legislation for the uptake of helmet use and prevention of head injuries. Cochrane Database of Systematic Reviews 2007, Issue 2. Art. No.: CD005401. DOI: 10.1002/14651858.CD005401 .pub2.Further information for this Cochrane review is available in this issue of EBCH in the accompanying EBCH Commentary article. Copyright © 2008 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Summary of ‘Human recombinant activated protein C for severe sepsis’
Authors: Milne Andrea 
Published: March 2008

AbstractThis is a summary of a Cochrane review, published in this issue of EBCH, first published as: Martí‐Carvajal A, Salanti G, Cardona AF. Human recombinant activated protein C for severe sepsis. Cochrane Database of Systematic Reviews 2008, Issue 1. Art. No.: CD004388. DOI: 10.1002/14651858.CD004388.pub3.Further information for this Cochrane review is available in this issue of EBCH in the accompanying EBCH Commentary and Characteristics and Key Findings Tables articles. Copyright © 2008 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Commentary on ‘Antihistamines and/or decongestants for otitis media with effusion (OME) in children’, with a reply from the review author
Authors: Smith Michael B. H. 
Published: March 2008

AbstractThis is a commentary on a Cochrane review, published in this issue of EBCH, first published as: Griffin GH, Flynn C, Bailey RE, Schultz JK. Antihistamines and/or decongestants for otitis media with effusion (OME) in children. Cochrane Database of Systematic Reviews 2006, Issue 4. Art. No.: CD003423. DOI: 10.1002/14651 858.CD003423.pub2.Further information for this Cochrane review is available in this issue of EBCH in the accompanying EBCH Summary and Characteristics and Key Findings Tables articles. Copyright © 2008 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Characteristics and key findings for ‘Antihistamines and/or decongestants for otitis media with effusion (OME) in children’
Authors: van Dalen E. C. , Offringa M. 
Published: March 2008

AbstractThese are the characteristics and key findings for a Cochrane review published in this issue of EBCH, first published as: Griffin GH, Flynn C, Bailey RE, Schultz JK. Antihistamines and/or decongestants for otitis media with effusion (OME) in children. Cochrane Database of Systematic Reviews 2006, Issue 4. Art. No.: CD003423. DOI: 10.1002/14651858.CD003423.pub2.Further information for this Cochrane review is available in this issue of EBCH in the accompanying EBCH Summary and Commentary articles. Copyright © 2008 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Summary of ‘Interventions for primary vesicoureteric reflux’
Authors: Milne Andrea 
Published: March 2008

AbstractThis is a summary of a Cochrane review, published in this issue of EBCH, first published as: Hodson EM, Wheeler DM, Vimalchandra D, Smith GH, Craig JC. Interventions for primary vesicoureteric reflux. Cochrane Database of Systematic Reviews 2007, Issue 3. Art. No.: CD001532. DOI: 10.1002/14651858.CD001532. pub3.Further information for this Cochrane review is available in this issue of EBCH in the accompanying EBCH Commentary and Characteristics and Key Findings Tables articles. Copyright © 2008 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Direct injection of organic solvent extracts for capillary electrophoresis
Authors: Shihabi Zak K. 
Published: March 2008

AbstractIt is demonstrated here that organic solvents immiscible in water used in sample extraction, such as chloroform, can be injected directly and successfully on the capillary without the need for evaporation and reconstitution. Current continuity was maintained all the time during the run. In order to avoid the rapid evaporation of the organic solvent during the analysis, the aqueous layer was left over the chloroform. This simplified the extraction step, and enabled the injection from the same vial over several hours without dealing with problem of evaporation. The relative peak heights in the electropherograms can be modified by the inclusion in the chloroform of a more polar solvent, by adjusting the pH, or adjusting the salt content of the sample. Addition of a polar solvent to the chloroform improved greatly the precision of the analysis for both the peak height and migration time.
Rapid capillary electrophoresis time‐of‐flight mass spectrometry separations of peptides and proteins using a monoquaternarized piperazine compound (M7C4I) for capillary coatings
Authors: Elhamili Anisa , Wetterhall Magnus , Arvidsson Björn , Sebastiano Roberto , Righetti Pier Giorgio , Bergquist Jonas 
Published: March 2008

AbstractA monoquaternarized piperazine, 1‐(4‐iodobutyl) 4‐aza‐1‐azoniabicyclo[2,2,2] octane iodide (M7C4I), has been evaluated as a surface derivatization reagent for CE in combination with TOF MS for the analysis of proteins, peptides, and protein digests. The M7C4I piperazine, at alkaline pH, forms a covalent bond via alkylation of the ionized silanols producing a cationic surface with a highly stable and reversed EOF. The obtained surface yields rapid separations (less than 5 min) of peptides and proteins at acidic pH with high separation efficiencies (up to 1.1×106 plates/m for peptides and up to 1.8×106 plates/m for proteins) and no observed bleeding of the coating reagent into the mass spectrometer. The simplicity of the coating procedure also enables fast (2 min) regeneration of the surface, if necessary. This is useful in the analysis of complex samples in order to prevent possible memory effects. The potential of using M7C4I‐coated capillaries for MS analysis of complex samples is demonstrated by the separation of peptides, proteins, and protein digests. Even more, the spectacular thing in which large intact proteins with molecular masses over 0.5 MDa could be separated. The coating showed good ability to handle these large proteins with high efficiency and retained peak shape as demonstrated by separation of IgG1 (150 kDa) and thyroglobulin (669 kDa).
Capillary electrophoresis of boron cluster compounds in aqueous and nonaqueous solvents
Authors: Valeri Anna Lucia , Kremser Leopold , Raggi Maria Augusta , Grüner Bohumir , Vespalec Radim , Kenndler Ernst 
Published: March 2008

AbstractThe electrophoretic properties of boron cluster compounds were determined in water, methanol and ACN as solvents of the BGE and discussed based on the principles of ion migration. Two types of boron cluster compounds were investigated. One type consisted of derivatives of the nido‐7,8‐dicarbaundecaborate cluster, the other types are derivatized cobalt bis(dicarbollide) ions (COSANs) whose central cobalt atom is sandwiched by two 7,8‐dicarbaundecaborate clusters. The BGE in all solvents was acetate/acetic acid buffer with pH 4.75 in water, 9.7 in methanol and 22.3 in ACN, respectively, at different ionic strength between 5 and 30 mM. The dependence of the mobility on ionic strength could not be explained by the theory of Debye, Hückel and Onsager, but good agreement was found upon considering an ion size parameter. Limiting mobilities were derived by curve fitting, and by the aid of the solvent viscosities the hydrodynamic radii of the analyte anions were calculated. They are between 0.25 and 0.48 nm, and were nearly independent of the solvent. Electrophoresis of the analytes in a BGE consisting of 6 mM perchloric acid in ACN allows the conclusion that the present boron cluster compounds behave as stronger acids than perchloric acid.
Combined effects of carbon, nitrogen and phosphorus substrates on D‐ribose production via transketolase deficient strain of Bacillus pumilus
Authors: Srivastava Rajesh K , Wangikar Pramod P 
Published: March 2008

AbstractBACKGROUND: D‐ribose, a five carbon sugar, can be produced via mutant strains with impairment in the non‐oxidative part of the pentose phosphate pathway (PPP). D‐ribose is an important intermediate in the pharmaceutical industry. This work focuses on the effect of various substrates on D‐ribose production with a transketolase deficient strain of Bacillus pumilus IFO13322.RESULT: D‐ribose titer is found to increase with a concomitant decrease in the yield (on a g product g−1 substrate basis) with increasing glucose concentration. Likewise, higher concentration of ammonium sulfate led to lower product yield while glucose and corn steep liquor concentrations were kept unaltered. Among the complex nitrogen substrates, cas amino acids showed better product yield than corn steep liquor. When tested for supplements of defined amino acids, media supplemented with tyrosine, phenylalanine, tryptophan and histidine gave better yields and higher biomass growth. This is possibly due to the fact that mutant strain is an aromatic amino acids auxotroph owing to impairment in aromatic amino acid biosynthesis. Further, higher phosphate concentrations in the media led to greater biomass formation and lower D‐ribose yield.CONCLUSIONS: At higher glucose or ammonium sulfate concentrations, a greater proportion of the carbon was channeled toward byproducts such as acetate, and acetoin and biomass formation. Thus, the relative rates of PPP and in turn the D‐ribose yield were dependent on not only the carbon substrate concentration but also the nitrogen and phosphorus substrates. Copyright © 2008 Society of Chemical Industry
Study of the bio‐production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using pre‐treated agro‐industrial substrates
Authors: Valduga Eunice , Valério Alexsandra , Treichel Helen , Di Luccio Marco , Furigo Júnior Agenor 
Published: March 2008

AbstractBACKGROUND: The increasing industrial demand for carotenoids has aroused interest in their bio‐production, and the need to reduce production costs has encouraged the use of low cost industrial substrates, such as agro‐industrial residues. Thus the objective of this research was the bio‐production of carotenoids by Sporidiobolus salmonicolor using agro‐industrial substrates (corn steep liquor and sugarcane molasses), pre‐treated with acids (sulphuric and phosphoric).RESULTS: Bio‐production was carried out in an orbital shaker using a 10% (v/v) inoculum, incubation at 25 °C, and agitation at 180 rpm for 120 h in a non‐illuminated environment. The carotenoids were recovered using liquid N2 combined with dimethylsulphoxide for cell rupture, and an acetone/methanol mixture (7:3 v/v) for extraction.CONCLUSION: The complete second‐order design allowed for optimisation of the carotenoid concentration obtained from industrial substrates pre‐treated with acids (sulphuric and phosphoric), obtaining a total carotenoid content of 541.5 µg L−1 using 10 g L−1 sugarcane molasses, 5 g L−1 corn steep liquor and 5 g L−1 yeast hydrolysate at 25 °C, with agitation at 180 rpm and an initial pH of 4.0. Copyright © 2008 Society of Chemical Industry
Recognition of the Helix‐Loop‐Helix domain of the Id proteins by an artificial luminescent metal complex receptor
Authors: Kiewitz Sebastian D. , Kruppa Michael , Riechers Alexander , König Burkhard , Cabrele Chiara 
Published: March 2008

AbstractSynthetic agents specifically interacting with a protein interface are important not only for the better understanding of protein dimer or complex formation but also for medical applications. Here we describe the recognition of the helix‐loop‐helix (HLH) dimerization domain of the Id proteins by an artificial luminescent receptor containing two binding sites for a Lewis acid and a Lewis base, respectively. The Id proteins are inhibitors of bHLH transcription factors and play key roles during development of cancer. We show that a receptor/Id‐HLH‐domain complex was formed cooperatively (K0.5 ∼2 µM under physiological conditions) and with moderate specificity, as compared to the related MyoD and Max HLH domains. Accordingly, a preferred receptor binding motif, CYSR(K), was identified within the Id HLH domains. These results are promising and may be exploited to design highly selective synthetic receptors for the Id HLH domain. Copyright © 2008 John Wiley & Sons, Ltd.
Conformational flexibility and kinetic complexity in antibody–antigen interactions
Authors: Kourentzi Katerina , Srinivasan Mohan , Smith‐Gill Sandra J. , Willson Richard C. 
Published: March 2008

AbstractThe kinetics of dissociation of three structurally characterized anti‐hen egg white lysozyme antibodies (H8, H10, and H26), with hen egg white lysozyme (HEL) and the avian variant Japanese quail lysozyme (JQL) were examined. These antibodies share over 90% sequence identity and recognize the same epitope, but differ in their degree of cross‐reactivity and predicted combining site rigidity. Competitive dissociation induced by the addition of excess unlabeled HEL after varied periods of antibody–antigen association was followed in real time using fluorescence anisotropy. Dissociation was in many cases non‐single‐exponential, and the observed off‐rates became slower as the complex age increased, suggesting multi‐step association kinetics consistent with an encounter‐docking view of protein–protein interactions. The fully docked fraction of the complexes just prior to inducing dissociation was high for the HEL complexes but was dramatically reduced for JQL complexes, that is final docking was antigen‐sensitive. Variations among the systems can be understood in terms of the complexes' differing conformational flexibilities, based on the encounter‐docking model of protein–protein associations. Copyright © 2008 John Wiley & Sons, Ltd.
A simple and convenient approach for evaluation of the parameters of ligand–receptor interaction. Receptor blocking index and its application
Authors: Bobrovnik S. A. 
Published: March 2008

AbstractA new approach for determination of the parameters for ligand‐receptor interaction, which is based on so‐called dilution coordinates, was developed earlier. Equations that allow evaluation of not only the affinity of ligand‐receptor interaction but also of the amount of free (or occupied by corresponding ligand) receptors were suggested. The most important advantage of this approach as compared with well‐known methods is the ability to determine the binding parameters for ligand‐receptor interaction even for the cases in which ligand and receptor are already present in a mixture and separation of counterparts from each other is technically difficult or even impossible. Due to this reason, the proposed approach can be especially useful for studying interactions between highly‐labile biological receptors and corresponding ligands as found in vivo. In the present paper I continue to consider how to determine the binding parameters for a given ligand‐receptor interaction if the value of receptor blocking index is determined experimentally. Copyright © 2008 John Wiley & Sons, Ltd.
Design, synthesis and screening of antisense peptide based combinatorial peptide libraries towards an aromatic region of SARS‐CoV
Authors: Huang Yanyan , Zhao Rui , Luo Jia , Xiong Shaoxiang , Shangguan Dihua , Zhang Hongwu , Liu Guoquan , Chen Yi 
Published: March 2008

AbstractA combination of high‐performance affinity chromatography and antisense peptide based combinatorial peptide libraries was used to screen a potential inhibitor for SARS‐CoV. An aromatic‐amino acid‐rich region within the transmembrane domain at the C terminal of spike (S) protein identified as a membrane‐active region was chosen as the target sense peptide (SP) and immobilized as affinity ligand. Four antisense peptides were designed based on the degeneracy of genetic codes. One of them was screened as the lead peptide to construct the extended peptide libraries (EPL). The library screening was carried out at pH 5.5 so as to mimic the low‐pH milieu required by virus fusion. After five cycles of screening, a dodecapeptide KKKKYRNIRRPG (DP) was identified to possess the highest binding affinity to the immobilized sense peptide. The dissociation constant of the complex between the DP and the SP was 5.64 × 10−7 M in a physiological condition. The recognition between the DP and recombinant SARS S protein was demonstrated by ELISA assay to be in a saturable way. The competitive inhibition of the sense peptide in the competitive ELISA reveals the affinity binding between the DP and SARS S protein is specific and directed towards the target SP of the S protein. The results indicate this preferred polypeptide can be used as a lead compound of potent inhibitor of SARS‐CoV. The mechanism study suggests the specific recognition between the DP and the target peptide was due to sequence‐dependent and multi‐modal affinity interaction. Copyright © 2008 John Wiley & Sons, Ltd.
Sensitivity of knee replacement contact calculations to kinematic measurement errors
Authors: Fregly Benjamin J. , Banks Scott A. , D'Lima Darryl D. , Colwell Clifford W. 
Published: March 2008

AbstractThe ability to measure in vivo knee kinematics accurately makes it tempting to calculate in vivo contact forces, pressures, and areas directly from kinematic data. However, the sensitivity of contact calculations to kinematic measurement errors has not been adequately investigated. To address this issue, we developed a series of sensitivity analyses derived from a validated in vivo computational simulation of gait. The simulation used an elastic foundation contact model to reproduce in vivo contact force, center of pressure, and fluoroscopic motion data collected from an instrumented knee replacement. Treating each degree of freedom (DOF) in the simulation as motion controlled, we first quantified how errors in measured relative pose of the implant components affected contact calculations. Pose variations of ±0.1 mm or degree over the entire gait cycle changed maximum contact force, pressure, and area by 204, 100, and 117%, respectively. Larger variations of ±0.5 mm or degree changed these same quantities by 1157, 108, and 578%, respectively. In both cases, the largest sensitivities were to errors in superior‐inferior translation and varus‐valgus rotation, with loss of contact occurring on one or both sides. We then quantified how switching the sensitive DOFs from motion to load control affected the sensitivity results. Pose variations of ±0.5 mm or degree in the remaining DOFs changed maximum contact quantities by at most 3%. These results suggest that accuracy on the order of microns and milliradians is needed to estimate contact forces, pressures, and areas directly from in vivo kinematic measurements, and that use of load rather than motion control for the sensitive DOFs may improve the accuracy of in vivo contact calculations. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1173–1179, 2008
Gender differences in passive knee biomechanical properties in tibial rotation
Authors: Park Hyung‐Soon , Wilson Nicole A. , Zhang Li‐Qun 
Published: March 2008

AbstractThe anterior cruciate ligament (ACL) is the most commonly injured knee ligament with the highest incidence of injury in female athletes who participate in pivoting sports. Noncontact ACL injuries commonly occur with both internal and external tibial rotation. ACL impingement against the lateral wall of the intercondylar notch during tibial external rotation and abduction has been proposed as an injury mechanism, but few studies have evaluated in vivo gender‐specific differences in laxity and stiffness in external and internal tibial rotations. The purpose of this study was to evaluate these differences. The knees of 10 male and 10 female healthy subjects were rotated between internal and external tibial rotation with the knee at 60° of flexion. Joint laxity, stiffness, and energy loss were compared between male and female subjects. Women had higher laxity (p = 0.01), lower stiffness (p = 0.038), and higher energy loss (p = 0.008) in external tibial rotation than did men. The results suggest that women may be at greater risk of ACL injury resulting from impingement against the lateral wall of the intercondylar notch, which has been shown to be associated with external tibial rotation and abduction. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:937–944, 2008