bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perinatal compromise affects development, form, and function of the hippocampus part two; preclinical studies
Authors: White Tegan A. , Miller Suzanne L. , Sutherland Amy E. , Allison Beth J. , Camm Emily J. 
Published: March 2024

Abstract
The hippocampus is a vital brain structure deep in the medial temporal lobe that mediates a range of functions encompassing emotional regulation, learning, memory, and cognition. Hippocampal development is exquisitely sensitive to perturbations and adverse conditions during pregnancy and at birth, including preterm birth, fetal growth restriction (FGR), acute hypoxic–ischaemic encephalopathy (HIE), and intrauterine inflammation. Disruptions to hippocampal development due to these conditions can have long-lasting functional impacts. Here, we discuss a range of preclinical models of prematurity and FGR and conditions that induce hypoxia and inflammation, which have been critical in elucidating the underlying mechanisms and cellular and subcellular structures implicated in hippocampal dysfunction. Finally, we discuss potential therapeutic targets to reduce the burden of these perinatal insults on the developing hippocampus.

Impact

The review explores the preclinical literature examining the association between pregnancy and birth complications, and hippocampal form and function.

The developmental processes and cellular mechanisms that are disrupted within the hippocampus following perinatal compromise are described, and potential therapeutic targets are discussed.
A let-7 microRNA-RALB axis links the immune properties of iPSC-derived megakaryocytes with platelet producibility
Authors: Chen Si Jing , Hashimoto Kazuya , Fujio Kosuke , Hayashi Karin , Paul Sudip Kumar , Yuzuriha Akinori , Qiu Wei-Yin , Nakamura Emiri , Kanashiro Maria Alejandra , Kabata Mio , Nakamura Sou , Sugimoto Naoshi , Kaneda Atsushi , Yamamoto Takuya , Saito Hirohide , Takayama Naoya , Eto Koji 
Published: March 2024

AbstractWe recently achieved the first-in-human transfusion of induced pluripotent stem cell-derived platelets (iPSC-PLTs) as an alternative to standard transfusions, which are dependent on donors and therefore variable in supply. However, heterogeneity characterized by thrombopoiesis-biased or immune-biased megakaryocytes (MKs) continues to pose a bottleneck against the standardization of iPSC-PLT manufacturing. To address this problem, here we employ microRNA (miRNA) switch biotechnology to distinguish subpopulations of imMKCLs, the MK cell lines producing iPSC-PLTs. Upon miRNA switch-based screening, we find imMKCLs with lower let-7 activity exhibit an immune-skewed transcriptional signature. Notably, the low activity of let-7a-5p results in the upregulation of RAS like proto-oncogene B (RALB) expression, which is crucial for the lineage determination of immune-biased imMKCL subpopulations and leads to the activation of interferon-dependent signaling. The dysregulation of immune properties/subpopulations, along with the secretion of inflammatory cytokines, contributes to a decline in the quality of the whole imMKCL population.
Height-renderable morphable tactile display enabled by programmable modulation of local stiffness in photothermally active polymer
Authors: Hwang Inwook , Mun Seongcheol , Youn Jung-Hwan , Kim Hyeong Jun , Park Seung Koo , Choi Meejeong , Kang Tae June , Pei Qibing , Yun Sungryul 
Published: March 2024

AbstractReconfigurable tactile displays are being used to provide refreshable Braille information; however, the delivered information is currently limited to an alternative of Braille because of difficulties in controlling the deformation height. Herein, we present a photothermally activated polymer-bilayer-based morphable tactile display that can programmably generate tangible three-dimensional topologies with varying textures on a thin film surface. The morphable tactile display was composed of a heterogeneous polymer structure that integrated a stiffness-tunable polymer into a light-absorbing elastomer, near-infra-red light-emitting diode (NIR-LED) array, and small pneumatic chamber. Topological expression was enabled by producing localized out-of-plane deformation that was reversible, height-adjustable, and latchable in response to light-triggered stiffness modulation at each target area under switching of stationary pneumatic pressure. Notably, the tactile display could express a spatial softness map of the latched topology upon re-exposing the target areas to modulated light from the NIR-LED array. We expect the developed tactile display to open a pathway for generating high-dimensional tactile information on electronic devices and enable realistic interaction in augmented and virtual environments.
Forward-predictive SERS-based chemical taxonomy for untargeted structural elucidation of epimeric cerebrosides
Authors: Tan Emily Xi , Leong Shi Xuan , Liew Wei An , Phang In Yee , Ng Jie Ying , Tan Nguan Soon , Lee Yie Hou , Ling Xing Yi 
Published: March 2024

AbstractAchieving untargeted chemical identification, isomeric differentiation, and quantification is critical to most scientific and technological problems but remains challenging. Here, we demonstrate an integrated SERS-based chemical taxonomy machine learning framework for untargeted structural elucidation of 11 epimeric cerebrosides, attaining >90% accuracy and robust single epimer and multiplex quantification with <10% errors. First, we utilize 4-mercaptophenylboronic acid to selectively capture the epimers at molecular sites of isomerism to form epimer-specific SERS fingerprints. Corroborating with in-silico experiments, we establish five spectral features, each corresponding to a structural characteristic: (1) presence/absence of epimers, (2) monosaccharide/cerebroside, (3) saturated/unsaturated cerebroside, (4) glucosyl/galactosyl, and (5) GlcCer or GalCer’s carbon chain lengths. Leveraging these insights, we create a fully generalizable framework to identify and quantify cerebrosides at concentrations between 10−4 to 10−10 M and achieve multiplex quantification of binary mixtures containing biomarkers GlcCer24:1, and GalCer24:1 using their untrained spectra in the models.
Large exchange-driven intrinsic circular dichroism of a chiral 2D hybrid perovskite
Authors: Li Shunran , Xu Xian , Kocoj Conrad A. , Zhou Chenyu , Li Yanyan , Chen Du , Bennett Joseph A. , Liu Sunhao , Quan Lina , Sarker Suchismita , Liu Mingzhao , Qiu Diana Y. , Guo Peijun 
Published: March 2024

AbstractIn two-dimensional chiral metal-halide perovskites, chiral organic spacers endow structural and optical chirality to the metal-halide sublattice, enabling exquisite control of light, charge, and electron spin. The chiroptical properties of metal-halide perovskites have been measured by transmissive circular dichroism spectroscopy, which necessitates thin-film samples. Here, by developing a reflection-based approach, we characterize the intrinsic, circular polarization-dependent complex refractive index for a prototypical two-dimensional chiral lead-bromide perovskite and report large circular dichroism for single crystals. Comparison with ab initio theory reveals the large circular dichroism arises from the inorganic sublattice rather than the chiral ligand and is an excitonic phenomenon driven by electron-hole exchange interactions, which breaks the degeneracy of transitions between Rashba-Dresselhaus-split bands, resulting in a Cotton effect. Our study suggests that previous data for spin-coated films largely underestimate the optical chirality and provides quantitative insights into the intrinsic optical properties of chiral perovskites for chiroptical and spintronic applications.
Water-participated mild oxidation of ethane to acetaldehyde
Authors: Li Bin , Mu Jiali , Long Guifa , Song Xiangen , Huang Ende , Liu Siyue , Wei Yao , Sun Fanfei , Feng Siquan , Yuan Qiao , Cai Yutong , Song Jian , Dong Wenrui , Zhang Weiqing , Yang Xueming , Yan Li , Ding Yunjie 
Published: March 2024

AbstractThe direct conversion of low alkane such as ethane into high-value-added chemicals has remained a great challenge since the development of natural gas utilization. Herein, we achieve an efficient one-step conversion of ethane to C2 oxygenates on a Rh1/AC-SNI catalyst under a mild condition, which delivers a turnover frequency as high as 158.5 h−1. 18O isotope-GC–MS shows that the formation of ethanol and acetaldehyde follows two distinct pathways, where oxygen and water directly participate in the formation of ethanol and acetaldehyde, respectively. In situ formed intermediate species of oxygen radicals, hydroxyl radicals, vinyl groups, and ethyl groups are captured by laser desorption ionization/time of flight mass spectrometer. Density functional theory calculation shows that the activation barrier of the rate-determining step for acetaldehyde formation is much lower than that of ethanol, leading to the higher selectivity of acetaldehyde in all the products.
A fungal core effector exploits the OsPUX8B.2–OsCDC48-6 module to suppress plant immunity
Authors: Shi Xuetao , Xie Xin , Guo Yuanwen , Zhang Junqi , Gong Ziwen , Zhang Kai , Mei Jie , Xia Xinyao , Xia Haoxue , Ning Na , Xiao Yutao , Yang Qing , Wang Guo-Liang , Liu Wende 
Published: March 2024

AbstractProteins containing a ubiquitin regulatory X (UBX) domain are cofactors of Cell Division Cycle 48 (CDC48) and function in protein quality control. However, whether and how UBX-containing proteins participate in host–microbe interactions remain unclear. Here we show that MoNLE1, an effector from the fungal pathogen Magnaporthe oryzae, is a core virulence factor that suppresses rice immunity by specifically interfering with OsPUX8B.2. The UBX domain of OsPUX8B.2 is required for its binding to OsATG8 and OsCDC48-6 and controls its 26 S proteasome–dependent stability. OsPUX8B.2 and OsCDC48-6 positively regulate plant immunity against blast fungus, while the high-temperature tolerance heat-shock protein OsBHT, a putative cytoplasmic substrate of OsPUX8B.2–OsCDC48-6, negatively regulates defense against blast infection. MoNLE1 promotes the nuclear migration and degradation of OsPUX8B.2 and disturbs its association with OsBHT. Given the high conservation of MoNLE1 among fungal isolates, plants with broad and durable blast resistance might be generated by engineering intracellular proteins resistant to MoNLE1.
Structure of the human Bre1 complex bound to the nucleosome
Authors: Onishi Shuhei , Uchiyama Kotone , Sato Ko , Okada Chikako , Kobayashi Shunsuke , Hamada Keisuke , Nishizawa Tomohiro , Nureki Osamu , Ogata Kazuhiro , Sengoku Toru 
Published: March 2024

AbstractHistone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 proteins generally function as tumor suppressors, while in certain cancers, they facilitate cancer cell proliferation. To obtain structural insights of H2BK120 ubiquitination and its regulation, we report the cryo-electron microscopy structure of the human Bre1 complex bound to the nucleosome. The two RING domains of Bre1A and Bre1B recognize the acidic patch and the nucleosomal DNA phosphates around SHL 6.0–6.5, which are ideally located to recruit the E2 enzyme and ubiquitin for H2BK120-specific ubiquitination. Mutational experiments suggest that the two RING domains bind in two orientations and that ubiquitination occurs when Bre1A binds to the acidic patch. Our results provide insights into the H2BK120-specific ubiquitination by the Bre1 proteins and suggest that H2B monoubiquitination can be regulated by nuclesomal DNA flexibility.
Spectroscopic visualization of reversible hydrogen spillover between palladium and metal–organic frameworks toward catalytic semihydrogenation
Authors: Liu Qiaoxi , Xu Wenjie , Huang Hao , Shou Hongwei , Low Jingxiang , Dai Yitao , Gong Wanbing , Li Youyou , Duan Delong , Zhang Wenqing , Jiang Yawen , Zhang Guikai , Cao Dengfeng , Wei Kecheng , Long Ran , Chen Shuangming , Song Li , Xiong Yujie 
Published: March 2024

AbstractHydrogen spillover widely occurs in a variety of hydrogen-involved chemical and physical processes. Recently, metal–organic frameworks have been extensively explored for their integration with noble metals toward various hydrogen-related applications, however, the hydrogen spillover in metal/MOF composite structures remains largely elusive given the challenges of collecting direct evidence due to system complexity. Here we show an elaborate strategy of modular signal amplification to decouple the behavior of hydrogen spillover in each functional regime, enabling spectroscopic visualization for interfacial dynamic processes. Remarkably, we successfully depict a full picture for dynamic replenishment of surface hydrogen atoms under interfacial hydrogen spillover by quick-scanning extended X-ray absorption fine structure, in situ surface-enhanced Raman spectroscopy and ab initio molecular dynamics calculation. With interfacial hydrogen spillover, Pd/ZIF-8 catalyst shows unique alkyne semihydrogenation activity and selectivity for alkynes molecules. The methodology demonstrated in this study also provides a basis for further exploration of interfacial species migration.
Cooling of a granular gas mixture in microgravity
Authors: Puzyrev Dmitry , Trittel Torsten , Harth Kirsten , Stannarius Ralf 
Published: March 2024

AbstractGranular gases are fascinating non-equilibrium systems with interesting features such as spontaneous clustering and non-Gaussian velocity distributions. Mixtures of different components represent a much more natural composition than monodisperse ensembles but attracted comparably little attention so far. We present the observation and characterization of a mixture of rod-like particles with different sizes and masses in a drop tower experiment. Kinetic energy decay rates during granular cooling and collision rates were determined and Haff’s law for homogeneous granular cooling was confirmed. Thereby, energy equipartition between the mixture components and between individual degrees of freedom is violated. Heavier particles keep a slightly higher average kinetic energy than lighter ones. Experimental results are supported by numerical simulations.
Data analytics and modelling in context to determination of moisture susceptibility of reclaimed asphalt foamed bituminous mix
Authors: Gupta Lokesh , Kumar Rakesh , Chakrabarti Tulika , Chakrabarti Prasun , Margala Martin 
Published: March 2024

AbstractThe presence of water badly affects the moisture susceptibility of the reclaimed asphalt Foamed Bituminous Mix (FBM). The present study is mainly emphasized to assess the moisture susceptibility of reclaimed asphalt FBM, Where RAP is being incorporated as a replacement of fresh aggregates. Moisture susceptibility of the mix is evaluated in terms of tensile strength ratio (TSR) and resilient modulus ratio, subjected to different conditioning procedures namely AASHTO T283, modified IDOT, TG-2 guidelines, and MIST. Further data analytics and regression modeling are also carried out to determine the moisture susceptibility of the mix and to check the statistics among the variables. The findings show that the incorporation of RAP in the FBM improves moisture resistance. Further, FBM containing 100% RAP shows the least moisture susceptibility in terms of TSR and Mr ratio irrespective of any conditioning type. Moreover, MIST conditioning may be preferred to assess the moisture sensitivity as it simulates the field pore pressure effects. Further, mathematical analysis is carried out to predict the moisture susceptibility of mix. Adjusted R square coefficient indicates a better fit of the prediction model developed. Overall, the study may be helpful to highway professionals in analyzing the conditioning procedures and determining the moisture sensitivity of the reclaimed asphalt Foamed Bituminous Mix.
Experimental design of 4-point supported belt robot for sanding large convex surfaces
Authors: Kim Hanbom , Jin Hongjoo , Lee Woojae , Chae SeungHeon , Kim Taegyun , Seo TaeWon 
Published: March 2024

AbstractIn general, sanding robots that move as if drawing a line along a surface are mainly used when sanding objects with a large area; however, they require a long working time, and it is difficult to secure a uniform sanded area. This study focuses on large-area sanding robots, such as those for ships, storage tanks, and tank lorries, and proposes an adaptive belt tension robot equipped with a 4-point supported belt mechanism capable of sanding variable curved surfaces. In addition, a sanding normal force prediction formula is proposed to describe the sanding performance of the contact surface. This equation consists of the concentrated load function due to the belt movement and the normal force due to the vertical and horizontal elongation of the belt. A video image analysis was performed to calculate the sanding area. Therefore, we determined whether the area was uniformly sanded. The dimensions of the test bench (W $$\times $$
×
D $$\times $$
×
H) were 1700 mm $$\times $$
×
1450 mm $$\times $$
×
900 mm. Experiments were performed using the proposed techniques on convex specimens with radii of 725, 1000, and 2100 mm. The sanding performance was improved by 43 $$\%$$
%
compared with that of a general belt-sanding robot.
Role of folate receptor α in the partial rejuvenation of dentate gyrus cells: Improvement of cognitive function in 21-month-old aged mice
Authors: Antón-Fernández A. , Cuadros R. , Peinado-Cahuchola R. , Hernández F. , Avila Jesús 
Published: March 2024

AbstractNeuronal aging may be, in part, related to a change in DNA methylation. Thus, methyl donors, like folate and methionine, may play a role in cognitive changes associated to neuronal aging. To test the role of these metabolites, we performed stereotaxic microinjection of these molecules into the dentate gyrus (DG) of aged mice (an average age of 21 month). Folate, but not S-Adenosyl-Methionine (SAM), enhances cognition in aged mice. In the presence of folate, we observed partial rejuvenation of DG cells, characterized by the expression of juvenile genes or reorganization of extracellular matrix. Here, we have also tried to identify the mechanism independent of DNA methylation, that involve folate effects on cognition. Our analyses indicated that folate binds to folate receptor α (FRα) and, upon folate binding, FRα is transported to cell nucleus, where it is acting as transcription factor for expressing genes like SOX2 or GluN2B. In this work, we report that a FRα binding peptide also replicates the folate effect on cognition, in aged mice. Our data suggest that such effect is not sex-dependent. Thus, we propose the use of this peptide to improve cognition since it lacks of folate-mediated side effects. The use of synthetic FRα binding peptides emerge as a future strategy for the study of brain rejuvenation.
A radiographic and histological study to compare red (650 nm) versus near infrared (810 nm) diode lasers photobiomodulation for alveolar socket preservation
Authors: Abd-Elhaleem Othman Mohamad Anwar , Zaky Ahmed Abbas , Eltayeb Elsayed Abdullah , Khalil Nesma Mohamed 
Published: March 2024

AbstractPrevious findings indicated that the laser photobiomodulation is more effective than the control or placebo in preserving the alveolar socket. This study aimed to compare two different lasers regarding their effectiveness in aiding alveolar socket preservation. Twenty extraction sockets were selected then divided into two equal groups. Group A was exposed to 650 nm Diode laser, and Group B to 810 nm Diode laser following the same protocol and parameters after a standard alveolar socket preservation procedure with collagen plug. Radiographic analysis with cone beam computed tomography was done to compare the alveolar bone surface area immediately after extraction and three months post-operatively, while bone samples collected before implant drilling were histologically examined for newly formed bone evaluation and histomorphometric analysis in terms of percentage of new bone surface area, percentage of unmineralized bone and finally, immunohistochemical analysis of Osteocalcin reaction surface area as well as optical density. Radiographically, infrared (810 nm) Diode effect on alveolar bone surface area has significantly exceeded the red laser, while histologically, red (650 nm) Diode has demonstrated statistical significance regarding all parameters; newly formed bone surface area percentage, unmineralized bone area percentage and finally Osteocalcin bone marker reaction surface area percentage and optical density. Under the specified conditions and laser parameters, photobiomodulation using the 810 nm Diode got the upper hand radiographically, yet histologically, the red 650 nm Diode managed to dominate all histological parameters when both employed as an adjunct to alveolar socket preservation procedures.
Volatile profile of bee bread
Authors: Pokajewicz Katarzyna , Lamaka Darya , Hudz Nataliia , Adamchuk Leonora , Wieczorek Piotr Paweł 
Published: March 2024

AbstractBee bread is one of the least studied bee products. In this study, ten bee bread samples were characterized using palynology and HS–SPME–GC–MS (headspace solid-phase microextraction gas chromatography-mass spectrometry). In total, over one hundred different volatile components were identified, belonging to different chemical groups. Only ten common components were detected in all the samples. These volatiles were ethanol, methylene chloride, ethyl acetate, acetic acid, α-pinene, furfural, nonane, nonanal, n-hexane and isovaleric acid. Several other components were commonly shared among various bee bread samples. Over sixty detected compounds have not been previously reported in bee bread. The analysis required a mild extraction temperature of 40 °C, as higher temperatures resulted in the Maillard reaction, leading to the production of furfural. The profile of volatile compounds of the tested bee pollen samples was complex and varied. Some relationships have been shown between botanical origin and volatile organic compound profile.
Experimental analysis and model prediction of elbow pipe's erosion in water-cooled radiator
Authors: Wang Yongfei , Li Xiaofei , Wang Tong , Zhang Jian , Li Longcheng , Zhang Yu 
Published: March 2024

AbstractThe radiator with heat transfer capability is able to guarantee the stable operation of hydro generator set, while the long-term and continuous scouring on radiator pipes by cooling medium will lead to thinning or even perforation of pipe wall, which triggers wall failure. This paper analyzes and predicts the failure mechanism of radiator’s pipe wall, and investigates the effects of water flow velocity, sand content and sand particle size on erosion damage of radiator pipe by establishing a test bench for pipe erosion. The results show that the increase of above parameters will lead to the increasing erosion rate, especially when the sand content is 1%, the velocity is 8 m/s and the sand particle size is 0.85 mm, the erosion damage will be particularly serious. Based on experimental data, BP and LSSVM models are employed to predict the pipe wall failure, and PSO algorithm is used to optimize the two models. The optimized PSO-BP has the highest accuracy with the mean absolute error (MAE) of 0.2070 and the mean absolute percentage error (MAPE) of 4.702%. The findings provide a reference for wall failure analysis of radiator, which is of great significance for unit's safe operation.