bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between retinal sensitivity and foveal microstructure after successful surgery for rhegmatogenous retinal detachment
Authors: Zhang Yanzhen , Wu Hailong , Hao Jianchen , Gu Xiaopeng , Zhang Yadi , zhang shijie 
Published: April 2024

Abstract

Purpose
To observe the correlation between retinal sensitivity, as measured by microperimetry, and foveal microstructure abnormalities in patients with rhegmatogenous retinal detachment (RRD) after successful surgery.
Methods
In this cross-sectional study, patients with primary unilateral RRD with retinal attachment more than three months after surgery were enrolled. Spectral-domain optical coherence tomography (SD-OCT) was used to observe changes in foveal microstructure. Mean retinal sensitivity (MS) and fixation stability were measured using microperimetry, and the correlation between MS and foveal microstructure abnormalities was analyzed.
Results
Thirty patients with RRD were included in this study (8 females and 22 males). Analysis of the foveal microstructure by SD-OCT revealed the formation of epiretinal membranes (ERMs) in 21 RRD eyes with RRD (70%), disruption of the external limiting membrane (ELM) in 10 RRD eyes (33%), disruption of the ellipsoid zone (EZ) in 9 RRD eyes (30%), and changes in the continuity of the interdigitation zone (IZ) in 18 RRD eyes with RRD (60%). The mean retinal sensitivity of the RRD eyes was significantly lower than that of the healthy fellow eyes (25.74 ± 1.60 vs. 27.86 ± 1.83 dB; p>0.01), and there was no significant difference in fixation stability between the eyes (p>0.05). There was a significant correlation between MS and disruption of the ELM (coefficient = −0.37, p = 0.04) and EZ (coefficient −0.39, p = 0.04).
Conclusions
The mean retinal sensitivity decreased in RRD patients with RRD after successful surgery, but the fixation stability was not abnormal. The decline in MS correlated with the disruption of ELM and EZ.
Beyond the stress response: absence of RelA protein hampers the cell wall structuring and cell size in Liquorilactobacillus vini
Authors: Santos Dayane da Silva , Mendonça Allyson Andrade , Zúñiga Manoel , de Morais Marcos Antonio , Elsztein Carolina 
Published: April 2024

Abstract

The role of (p)ppGpp alarmone produced by RelA protein is well documented as a response to stress and nutritional deficiency in bacteria. However, little information is reported for Lactobacillus sensu lato, including the remaining species of old genus Lactobacillus and the derived species recently allocated in new genera. The present study aimed to characterize for the first time the effect of the inactivation of relA in a representative of the Liquorilactobacillus genus. Results obtained have revealed an unexpected role of RelA protein on the cell wall homeostasis and cell size. L.vini ΔrelA showed low growth and increased cell size, related to overexpression of genes responsible for DNA transcription and repair and down-expression of the fusA gene. The low growth also resulted in the loss of the flagellum in ΔrelA, which may also be associated with the fragility of the cell wall, ease of lysis and resistance to beta-lactams of the ΔrelA mutant. Cell wall homeostasis was deregulated mainly by the down-expression of the gene encoding alanine ligase and murB, which may have triggered the overexpression of the genes encoding PBPs proteins. Growth data suggest that the absence of the RelA protein promoted a disconnection between the cell cycle and cell division in L. vini, and a consequent reduction in the growth rate of the culture, cell wall fragility and beta-lactam resistance, expanding the RelA function in Liquorilactobacillus beyond the stringent response.
Investigating Gender Disparities in the Relationships between Psychological Hardiness and Subjective Well-being in Cardiac Patients: The Mediating Role of Resilience
Authors: Muhammad Zohaib Khan  , Syeda Shahida Batool  
Published: April 2024

Background: Health psychologists are constructively playing their role to help us know the causes of psychobiological disorders that affect human physical and psychological health
Objective: The existing study was designed to investigate the gender differences among cardiac patients in terms of psychological hardiness, subjective well-being, and resilience in cardiac patients.
Methods: Gender differences among cardiac patients were investigated using a cross-sectional study approach. The research comprised 75 cardiac patients (43 male and 32 female) ranging in age from 27 + to 65 years. Participants were briefed about the significance of the research, informed consent was obtained, and data were collected using valid, indigenously translated research instruments. Patient anonymity was maintained, and responses were evaluated using SPSS-23 and AMOS-20 statistical tools.
Results: Results from the independent sample t-test revealed that male cardiac patients exhibited a significantly higher level of psychological hardiness (t=4.46, p<.05) and resilience (t=7.51, p<.05) compared to their female counterparts. However, the study found no statistically significant gender differences in subjective well-being. (t=.08, p>.05). Moreover, path analysis was conducted through structure equational modelling, unveiled that resilience played a significant role in the connection between psychological hardiness and subjective well-being in the patients (χ2=1.82, p<.05, RMSEA=.07, RMR=.20, CFI=.97, GFI=.98, and TLI=.93). The determined value of chi-square (χ2/pdf) 1.82 is acceptable for model fit indexes parameters. The multiple linear regression analysis findings revealed that resilience and psychological hardiness were significant (p<.05) predictors of subjective well-being in cardiac patients.
Conclusion: This research emphasizes the vital role of psychological hardiness in cardiac patients' lives, showcasing its adaptive function. The study's findings will contribute to the existing literature, providing valuable guidance for medical professionals, health/clinical psychologists, and the families of patients. This information aims to enhance rehabilitation strategies and promote improved well-being for individuals with cardiac patients.
To Determine the Frequency of Clinical Remission Induction with Versus without Fecal Microbiota Transplant in Treatment of Active Ulcerative Colitis
Authors: Bakr Attique Abou , Sarwar Shahid , Aslam Muhammad Naeem , Ghias Mamoona , Yousuf Muhammad Nadeem 
Published: April 2024

Background: Ulcerative colitis is a long lasting inflammatory disease of the intestines that gets worse over time.
Objective:. The goal of this study was to compare the success rates of inducing clinical remission in patients with active Ulcerative Colitis who underwent treatment with or without Fecal Microbiota Transplant.
Methods: It was a Randomized Controlled Trial, which was done in Department of Gastroenterology/Medical Unit II of Jinnah Hospital Lahore .Study duration was 18months (from 01-01-2021 to 30-06-2022). The research included 90 patients who were randomly assigned to two groups. Standard Ulcerative Colitis therapy was given to both groups. Patients in group A recieved once-weekly faecal microbiota transplant for a period of six weeks. Patients in group B had weekly water enemas for same period. All patients underwent colonoscopy and Mayo score was noted before treatment, and after 6 weeks of treatment. Clinical remission was labeled if patients achieved Mayo score £2 after treatment.
Results: Group A (Treatment) had a mean age of 39.31 ±13.45 and group B (Control) had a mean age of 38.59 ±12.11 (p=0.52). Duration of ulcerative colitis in group A was 8.08±2.64 weeks and in group B was 9.00±2.62 weeks (p=0.76). Pre-treatment Mayo score in groups A and B, were 8.29±1.91in and 8.73 ±1.61 respectively (p=0.15). Post-treatment in group A, the Mayo score was 3.71± 2.22, whereas in group B, it was 5.07± 1.75 (p=0.003). Remission was achieved in 24.44% (n=11) in fecal microbiota transplant group and 8.88% (n=4) in control group. (p=0.003).
Conclusion: When compared to the control group, FMT significantly induces remission in patients with active UC.
Evolution of Prime Editing Systems: Move Forward to the Treatment of Hereditary Diseases
Authors: Volodina Olga V. , Fabrichnikova Anastasia R. , Anuchina Arina A. , Mishina Olesya S. , Lavrov Alexander V. , Smirnikhina Svetlana A. 
Published: April 2024

:
The development of gene therapy using genome editing tools recently became relevant.
With the invention of programmable nucleases, it became possible to treat hereditary diseases due
to introducing targeted double strand break in the genome followed by homology directed repair
(HDR) or non-homologous end-joining (NHEJ) reparation. CRISPR-Cas9 is more efficient and
easier to use in comparison with other programmable nucleases. To improve the efficiency and
safety of this gene editing tool, various modifications CRISPR-Cas9 basis were created in recent
years, such as prime editing – in this system, Cas9 nickase is fused with reverse transcriptase and
guide RNA, which contains a desired correction. Prime editing demonstrates equal or higher correction
efficiency as HDR-mediated editing and much less off-target effect due to inducing nick.
There are several studies in which prime editing is used to correct mutations in which researchers
reported little or no evidence of off-target effects. The system can also be used to functionally
characterize disease variants. However, prime editing still has several limitations that could be further
improved. The effectiveness of the method is not yet high enough to apply it in clinical trials.
Delivery of prime editors is also a big challenge due to their size. In the present article, we observe
the development of the platform, and discuss the candidate proteins for efficiency enhancing,
main delivery methods and current applications of prime editing.
Pulmonary Hypertension Associated with Chronic Lung Disease
Authors: Wang Yuanchen , Estrada Rodolfo A. , Goel Khushboo , Folz Rodney J. , Sahay Sandeep 
Published: April 2024

Abstract:
Patients with Chronic Lung Disease (CLD) are frequently burdened by pulmonary hypertension
(PH), which is associated with reduced functional capacity, poor quality of life, increased
oxygen requirements, and increased morbidity and mortality. The development of PH associated
with chronic lung disease (PH-CLD) is complex and multifactorial and varies between
different types of CLD. In this review, we provide an update on PH-CLD, with a particular focus
on Interstitial Lung Disease (ILD), chronic obstructive pulmonary disease (COPD), obstructive
sleep apnea (OSA), and obesity hypoventilation syndrome (OHS). We discussed epidemiology,
histopathology, pathophysiology, diagnostic evaluation, and treatment approaches. There are limited
data on the use of pulmonary arterial hypertension-specific treatments in PH-CLD, so it has
been proposed to phenotype patients based on their degree of pulmonary vascular disease to guide
individualized care. The heterogeneity within PH-CLD highlights the importance of identifying
novel molecular pathways unique to each subgroup to ultimately achieve precision medicine.
Therapeutic Potential of Dicentrine in Human Disorders: A Review On Medicinal Importance and Pharmacological Activities of Aporphine Alkaloid
Authors: Patel Kanika , Patel Dinesh Kumar 
Published: April 2024

Backgrounds:
Plants have always been an important source of food and drugs, in addition
to their application in medical and cosmetic sectors. The popularity of herbal remedies has increased
in many countries in recent decades. Herbal medicines contain numerous active phytocomponents
with different biological activities. Alkaloid class phytochemicals have diverse chemical structures
and pharmacological activities in nature. Additionally, Aporphinoids are an important class of plant
secondary metabolites that have been used for the treatment of numerous human disorders for a long
time in traditional medicine. Dicentrine is an aporphine class phytochemical isolated from numerous
medicinal plants, including Actinodaphne sesquipedalis and Lindera megaphylla.

Methods:
The biological potential of dicentrine, an aporphine alkaloid derivative, has been described
in the present work. Scientific data on dicentrine were collected here from different scientific databases and presented in this paper in order to know the biological importance of dicentrine in medicine for the treatment of human complications. Further, detailed pharmacological activities and scientific data on dicentrine were also analyzed in order to determine its therapeutic potential in medicine. Moreover, its analytical aspects were also described in this work to understand its separation
and isolation methods.

Results:
The present work described the biological potential of dicentrine in medicine and its analytical aspects. It signified the biological potential of dicentrine in cancer, breast cancer, oral squamous
cell carcinoma, lung adenocarcinoma, blood pressure, inflammatory disorders, hyperlipidaemia,
arrhythmia, stomach muscle, and glomerulonephropathy. Further, its effectiveness in medicine was
found to be mainly because of its antiplatelet, alpha 1-adrenoceptor, epidermal growth factor, antiprotozoal, larvicidal, antimicrobial, topoisomerase II, and acetylcholinesterase inhibitory potential.
Moreover, other scientific data also signified its metabolism and pharmacokinetic parameters in
terms of its analytical aspects in medicine.

Conclusion:
The present review gives us an updated summary of the scientific information for
pharmacological activities and analytical aspects of dicentrine in medicine. It also signified the potential contribution of dicentrine in medicine for the development of a newer class of drug molecules
for human disorders.
Exploration of the Molecular Mechanism Underlying the Antidiabetic Activity of a Northeast Indian Plant Zingiber rubens Roxb. through Modulation of PKC Phosphorylation
Authors: Das Sujit , Devroy Piyali , Chatterjee Suraj Kumar , Jana Sandipan , Gayen Srijon , Ghosh Suparna , Bhowmik Manas , Bala Asis , Haldar Pallab Kanti 
Published: April 2024

Background:
Zingiber rubens Roxb., a new species of the Zingiberaceae family, is found
profoundly in the Northeastern region of India. It is a rhizomatous geophyte and grows primarily in
the seasonally dry tropical biome native to Indo-China regimens.

Objective:
The aim is to research the root part of this plant to discover active constituents and evaluate the molecular mechanism of antidiabetic activity

Methods:
The hydroalcoholic root extract of this plant (HAZR) was evaluated for in-vitro antioxidant assays α-amylase and α-glucosidase inhibition assay. Further, the antidiabetic activity assay was
evaluated, exploring the molecular mechanism for modulating oxidative stress in tissue biochemical
parameters and HbA1c. To explore the molecular mechanism, we also investigated the inhibition
assay of protein kinase C (PKC) phosphorylation in mouse peritoneal macrophages.

Results:
The experiments' results helped to conclude that HAZR has potent antioxidant and antidiabetic activity and could have been embedded with potent active molecules. They also revealed the
mechanism of action as inhibition of PKC phosphorylation, meriting further studies.

Conclusion:
The present study revealed that HAZR demonstrated its antidiabetic activity against
diabetic animal models through oxidative stress-mediated PKC and TGF-β regulation in diabetic
individuals.
Effect of Plant Secondary Metabolites against Lung Cancer: A Comprehensive Review on Apoptosis Induction and Synergistic Potential
Authors: Dewangan Nirupama Rani , Agrawal Prachi , Sahu Priyanka , Sahu Dalchand , Yadav Megha , Gupta Priyanka , Ahirwar Bharti 
Published: April 2024

:
Lung carcinoma is the most ubiquitous and fundamental cause of cancer-related mortality
globally. The conventional therapeutic approaches for lung cancer have consistently encountered
several challenges and complexities, prompting the exploration of novel tactics for the detection and
management of cancer. Lung cancer is a highly preventable disease, and early-stage detection significantly
improves patient’s survival rates. Several distinct medicinal substances from plants aid in
treating human illnesses and promoting good health. Plants, including Astragalus propinquus, Curcuma
longa, Anethum graveolens, Allium cepa, etc., are utilized as medicines to treat lung cancer. It
can be effectively treated using plant secondary metabolites like flavonoids, phenolics, terpenoids,
steroids, alkaloids, and polyphenols. This review describes the structures of plant metabolites and the
process by which they induce apoptosis against lung cancer. This data will assist in the comprehensive
awareness and understanding of the factors that combat phytochemicals against lung cancers
and the production of new anti-cancer medications.
Plastic Waste Valorization: Prospects for Green Hydrogen Production
Authors: Luque Rafael 
Published: April 2024

:
Plastic waste is a current issue worldwide that is already negatively influencing and
threatening the lives of human beings, with residual micro- and nanoplastics entering water and soil
bodies inducing recalcitrant pollution and health issues. The proposed perspective has been aimed to
provide an overview of the potential of plastic waste valorization to green hydrogen and carbonaceous
nanostructures. The overall concept additionally includes the utilization of the generated carbonaceous
nanostructures to design advanced functional materials in combination with the obtained
green hydrogen from plastic waste in a number of batch-to-flow catalytic hydrogenations to close the
circle of sustainable integrated valorization of plastic waste. The concept also includes insightful
Life-Cycle Assessment (LCA) and techno-economic studies in order to select the most relevant lines
from the sustainability and cost-competitive standpoints.
Social media for research dissemination: A research network’s Twitter (X) journey and development of a logic model framework for knowledge translation (Preprint)
Authors: Hooley Gwendolyn C , Lin Michelle , Magana Julia N , Woods Jason M , Sivasankar Shyam , VonHoltz Lauren , Schmidt Anita R , Chang Todd P 
Published: April 2024

BACKGROUND

Research dissemination is a vital step in bridging the gap between the publication of cutting edge research and its adoption into clinical practice. Social media is gaining increasing prominence in academic medicine as a way to engage, educate, and accelerate knowledge translation. Guidance on best practices is needed for research organizations and medical institutions hoping to launch professional social media accounts.

OBJECTIVE

To provide a roadmap for organizations creating a professional Twitter account for research dissemination.

METHODS

This was a mixed methods study. A qualitative approach collected the Pediatric Emergency Care Applied Research Network’s (PECARN) Twitter team's experience from 2020 to 2023. We initially extracted insights from team members' experiences using a Nominal Group Technique and Slack communications. Subsequently we analyzed quantitative data from Twitter performance metrics and Altmetrics Attention Scores for feature journal publications. The two datasets informed the design of a logic model, offering a practical framework for research organizations planning to use Twitter for research dissemination.

RESULTS

Five stakeholders generated 63 thematic statements that coalesced into 9 categories of recommendation statements that formulated a logic model. We provide recommendations for each aspect, overlaid by our experiences and relevant analytic data. We also describe the priorities, assumptions, and external factors which influenced our decision and findings.

CONCLUSIONS

Highlighting the role of social media in knowledge translation, we describe the importance of a strategic Twitter presence for research organizations and medical institutions. Our logic model roadmap, based on our practical multi-year experience and data-driven strategies, hopefully can serve as a guide for organizations aiming to incorporate Twitter for research dissemination.
Effects of Sodium-Glucose Cotransporter 2 Inhibitors on Cause-Specific Cardiovascular Death in Patients with CKD
Authors: Fletcher Robert A. , Herrington William G. , Agarwal Rajiv , Mayne Kaitlin J. , Arnott Clare , Jardine Meg J. , Mahaffey Kenneth W. , Perkovic Vlado , Staplin Natalie , Wheeler David C. , Chertow Glenn M. , Heerspink Hiddo J.L. , Neuen Brendon L. 
Published: April 2024

Clinical Trial registry name and registration number: ClinicalTrials.gov Identifiers: NCT02065791 (CREDENCE), NCT03036150 (DAPA-CKD), NCT03594110 (EMPA-KIDNEY).

Podcast
This article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2024_09_23_CJASNSeptember19992.mp3
Importancia del ciclo biogeoquímico del aluminio (Al) con relación con la acidez de los suelos en la producción piscícola y la salud pública ¿cuál sería el caso de la Orinoquia?
Authors: RONDON BARRAGÁN I.S. , RAMÍREZ DUARTE W.F. , BARATO P. , ESLAVA MOCHA P.R. 
Published: April 2024

Titulo en ingles: Importance of the biogeochemical cycle of aluminium (Al) with relationship with soil acidity in the fish production and public health What would be Orinoquia’s case?RESUMEN: Los hallazgos de patología branquial en peces cultivados en los Llanos Orientales, han sugerido que factores medio ambientales pueden favorecer la presentación de dichas lesiones antes de la colonización de microorganismos. Teniendo en cuenta condiciones de acidez, alto contenido de aluminio (Al) intercambiable de los suelos y además baja condición de dureza de agua de la Orinoquia. Se ha reportado que peces en estas condiciones ambientales pueden presentar problemas asociados a la toxicidad de metales pesados como alteraciones ionorregulatorias y respiratorias, entre otros. Razón por la cual se ha propuesto estudiar el posible papel del aluminio. Además, el Al ha sido relacionado con el desarrollo de enfermedades neurodegenerativas como la enfermedad de Alzheimer en humanos frente a lo cual también se deben adelantar estudios. Por lo anterior, se propuso como objetivo de esta revisión de literatura describir algunas características biogeoquímicas del Al así como su interacción con los demás sistemas biológicos, especialmente los peces y su implicación en el desarrollo de enfermedades neurodegenerativas de importancia, se enfatiza en los temas relacionados con las condiciones ambientales orinoquences.Palabras clave: Aluminio, toxicología acuática, ciclos biogeoquímicos, producción acuícola.ABSTRACT: Findings in gill pathology from fish cultured in oriental plains, have suggested that environmental factors can facilitate the expression of such lesions before the colonization of microorganisms. Take account the acidity, high content of exchangeable aluminium (Al) in soils as well as low hardness in the water from Orinoquia. Problems in fish under these environmental conditions have been reported showing heavy metal-associated ionoregulatory and respiratory alterations, for example. Furthermore, Al have been associated with neurodegenerative diseases such as Alzheimer disease in humans by which many studies must be done. Thus, the aim of this review is to describe some biogeochemical features of Al as well as its interaction with other biological systems, especially fish and its implications in the development of neurodegenerative diseases, emphasizing in Orinoquia’s environmental conditions.Keywords: Aluminium; aquatic toxicology; biogeochemical cycle; aquaculture.