bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generation of a tightly regulated doxycycline‐inducible model for studying mouse intestinal biology
Authors: Roth Sabrina , Franken Patrick , van Veelen Wendy , Blonden Lau , Raghoebir Lalini , Beverloo Berna , van Drunen Ellen , Kuipers Ernst J. , Rottier Robbert , Fodde Riccardo , Smits Ron 
Published: October 2008

AbstractTo develop a sensitive and inducible system to study intestinal biology, we generated a transgenic mouse model expressing the reverse tetracycline transactivator rtTA2‐M2 under control of the 12.4 kb murine Villin promoter. The newly generated Villin‐rtTA2‐M2 mice were then bred with the previously developed tetO‐HIST1H2BJ/GFP model to assess inducibility and tissue‐specificity. Expression of the histone H2B‐GFP fusion protein was observed exclusively upon doxycycline induction and was uniformly distributed throughout the intestinal epithelium. The Villin‐rtTA2‐M2 was also found to drive transgene expression in the developing mouse intestine. Furthermore, we could detect transgene expression in the proximal tubules of the kidney and in a population of alleged gastric progenitor cells. By administering different concentrations of doxycycline, we show that the Villin‐rtTA2‐M2 system drives transgene expression in a dosage‐dependent fashion. Thus, we have generated a novel doxycycline‐inducible mouse model, providing a valuable tool to study the effect of different gene dosages on intestinal physiology and pathology. genesis 47:7–13, 2009. © 2008 Wiley‐Liss, Inc.
The Pax3‐Cre transgene exhibits a rostrocaudal gradient of expression in the skeletal muscle lineage
Authors: Jarad George , Miner Jeffrey H. 
Published: October 2008

AbstractPax3‐Cre (P3Pro‐Cre) transgenic mice have been used for conditional gene deletion and/or lineage tracing in derivatives of neural crest, neural tube, metanephric mesenchyme, and ureteric mesenchyme. However, the extent of its expression in skeletal muscle has not been reported. We investigated the expression of P3Pro‐Cre in the skeletal muscle lineage using the R26R reporter and found an unexpected rostrocaudal gradient of expression. By X‐gal staining, head, neck, forelimb, diaphragm, and most of the chest wall muscles did not show evidence of Cre expression, whereas all muscle groups posterior of the diaphragm stained blue. Intercostal muscles exhibited a rostrocaudal gradient of staining. The consistency of this expression pattern was demonstrated by using P3Pro‐Cre to mutate a conditional dystroglycan allele. The result was loss of dystroglycan from caudal muscles, which exhibited the histological signs of muscle fiber injury and regeneration characteristic of muscular dystrophy. The lack of dystroglycan in regenerating myofibers suggests that the P3Pro‐Cre transgene is active in satellite cells and/or in their precursors. In contrast, rostral muscles, including feeding and breathing muscles, maintained dystroglycan expression and were spared from disease. Accordingly, the mutants were viable for over a year. Its unique gradient of activity makes the P3Pro‐Cre transgene a previously unappreciated yet powerful tool for manipulating gene expression in skeletal muscle and its precursors. genesis 47:1–6, 2009. © 2008 Wiley‐Liss, Inc.
Predictors of response to cognitive behavioral treatment for bulimia nervosa delivered via telemedicine versus face‐to‐face
Authors: Marrone Sonia , Mitchell James E. , Crosby Ross , Wonderlich Steve , Jollie‐Trottier Tami 
Published: October 2008

AbstractObjective:This study presents the results of a receiver operating characteristics (ROC) analysis to evaluate response to cognitive behavioral treatment (CBT) for patients with bulimia nervosa (BN), delivered via telemedicine (TV‐CBT) or face‐to‐face (FTF‐CBT).Method:Data were gathered on 116 adults treated with CBT for BN. Response to treatment (i.e., percent reduction in binge eating and purging behavior) were examined at weeks two, four, six, and eight. ROC analysis was completed to predict abstinence at end of treatment (EOT) as well as 3‐month and 1‐year follow‐up for the entire sample and by treatment group (TV‐CBT versus FTF‐CBT).Results:ROC analyses revealed that abstinence at EOT and 1‐year follow‐up was predicted by percent reduction in binge eating behavior whereas abstinence at 3‐month follow‐up was predicted by percent reduction in purging behavior. Results showed differences in predictors of treatment response when ROC analyses were completed for the entire sample and by treatment group.Discussion:Results suggest that evaluating percent reduction in binge eating and purging at weeks two, four, six, and eight of treatment is a clinically useful tool for predicting treatment response at EOT, 3‐month, and 1‐year follow‐up. © 2008 by Wiley Periodicals, Inc. Int J Eat Disord 2009
Di‐ or Trinuclear 3d–4f Schiff Base Complexes: The Role of Anions
Authors: Costes Jean‐Pierre , Donnadieu Bruno , Gheorghe Ruxandra , Novitchi Ghenadie , Tuchagues Jean‐Pierre , Vendier Laure 
Published: October 2008

AbstractWe demonstrate through structural, spectroscopic, and magnetic studies that the main factors governing the nuclearity of M–Gd (M = Cu, Ni) complexes derived from compartmental Schiff base ligands are the different affinities of the lanthanide ions for the potential ligands and anions present in the reaction medium. In the eight examples studied, there is competition between the tetradentate O2O2 coordination site of the polydentate ligand and the anionic entities brought by the gadolinium salts. The strong affinity of nitrato anions for lanthanides yields dinuclear complexes and prevents formation of trinuclear entities, whereas the use of poorly coordinating anions such as triflates may yield either dinuclear or trinuclear complexes, depending on the 3d/4f ratio.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Spin Canting in a Cobalt(II) Radical Complex with an Acentric Counter Anion (Eur. J. Inorg. Chem. 31/2008)
Authors: Mitsumoto Kiyotaka , Shiga Takuya , Nakano Motohiro , Nihei Masayuki , Nishikawa Hiroyuki , Oshio Hiroki 
Published: October 2008

AbstractThe cover picture shows that cobalt(II) diradical complexes with centric and acentric counter anions, [CoCl(bisimpy)(MeOH)2]X (X = PF6 and ClO4), crystallize in the centrosymmetric and polar space groups of P21c and Pna21, respectively. The PF6– salt is paramagnetic down to 1.8 K, whereas the ClO4– salt shows a weak ferromagnetic long‐range order with metamagnetic behavior at 1.8 K. Spin canting can arise from single‐ion magnetic anisotropy and/or antisymmetric exchange interaction (Dzyaloshinsky–Moriya interaction). The noncentrosymmetric space group of the latter complex is compatible with both mechanisms. Details are discussed in the Short Communication by H. Oshio et al. on p. 4851 ff.
Synthesis of 3‐Deoxy Glycals via Tandem Metathesis Sequences and Their Use in an Intermolecular Heck Arylation
Authors: Schmidt Bernd , Biernat Anne 
Published: October 2008

AbstractDeoxy glycals can be synthesized from a mannitol‐derived C2‐symmetric diene diol by using the tandem RCM/isomerization approach. Incorporation of a ruthenium‐catalyzed dehydrogenative silylation step into the reaction sequence is possible. Thus, an orthogonally protected 3‐deoxy glycalresults via a tandem RCM/hydrosilylation/isomerization sequence. All ruthenium‐catalyzed steps of this tandem sequence occur under distinct conditions, allowing for the selective synthesis of the intermediates. The 3‐deoxy glycals obtained via this route undergo a regio‐ and stereoselective Heck reaction, as exemplified by the synthesis of a C‐aryl glycoside. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
HAMLET (human α‐lactalbumin made lethal to tumor cells) triggers autophagic tumor cell death
Authors: Aits Sonja , Gustafsson Lotta , Hallgren Oskar , Brest Patrick , Gustafsson Mattias , Trulsson Maria , Mossberg Ann‐Kristin , Simon Hans‐Uwe , Mograbi Baharia , Svanborg Catharina 
Published: October 2008

AbstractHAMLET, a complex of partially unfolded α‐lactalbumin and oleic acid, kills a wide range of tumor cells. Here we propose that HAMLET causes macroautophagy in tumor cells and that this contributes to their death. Cell death was accompanied by mitochondrial damage and a reduction in the level of active mTOR and HAMLET triggered extensive cytoplasmic vacuolization and the formation of double‐membrane‐enclosed vesicles typical of macroautophagy. In addition, HAMLET caused a change from uniform (LC3‐I) to granular (LC3‐II) staining in LC3‐GFP‐transfected cells reflecting LC3 translocation during macroautophagy, and this was blocked by the macroautophagy inhibitor 3‐methyladenine. HAMLET also caused accumulation of LC3‐II detected by Western blot when lysosomal degradation was inhibited suggesting that HAMLET caused an increase in autophagic flux. To determine if macroautophagy contributed to cell death, we used RNA interference against Beclin‐1 and Atg5. Suppression of Beclin‐1 and Atg5 improved the survival of HAMLET‐treated tumor cells and inhibited the increase in granular LC3‐GFP staining. The results show that HAMLET triggers macroautophagy in tumor cells and suggest that macroautophagy contributes to HAMLET‐induced tumor cell death. © 2008 Wiley‐Liss, Inc.
Treatment outcomes and clinicopathologic characteristics of triple‐negative breast cancer patients who received platinum‐containing chemotherapy
Authors: Uhm Ji Eun , Park Yeon Hee , Yi Seong Yoon , Cho Eun Yoon , Choi Yoon La , Lee Su Jin , Park Min Jae , Lee Se‐Hoon , Jun Hyun Jung , Ahn Jin Seok , Kang Won Ki , Park Keunchil , Im Young‐Hyuck 
Published: October 2008

AbstractThe aim of this study was to evaluate the role of platinum‐containing chemotherapy for metastatic triple‐negative breast cancer (TNBC) patients in terms of the response rate (RR) and progression‐free survival. A second aim was to characterize the clinical behavior at the time of relapse of TNBC. We retrospectively analyzed the clinical outcomes of patients with metastatic breast cancer who received taxane–platinum chemotherapy as the first‐ or second‐line treatment, focusing on the TN phenotype. In total, 257 patients with metastatic breast cancer received platinum‐containing chemotherapy at Samsung Medical Center from 1999 to 2006. Of these patients, 106 patients with available data on estrogen (ER), progesterone (PgR) and human epidermal growth factor receptor‐2 (HER2) receptor status received taxane–platinum regimen as the first‐ or second‐line treatment. The overall RR of patients with TNBC was 39%. This rate did not differ significantly from those of patients with other phenotypes. The time to death after chemotherapy (19 vs. 50 months, p = 0.037) and overall survival (OS) (21 vs. 56 months, p = 0.030) differed significantly between patients with TNBC and non‐TNBC. TNBC showed a unique locoregional infiltration pattern at relapse, which might reflect its aggressive clinical behavior. Despite the similar response to platinum‐containing chemotherapy, patients with TNBC had a shorter OS than patients with non‐TNBC. The implication of TN phenotype as poor prognostic factor is uncertain, because it needs to be defined whether poor outcome is related to the rapid growing characteristics of tumor itself or the resistance to drug therapy. Further prospective studies are warranted. © 2008 Wiley‐Liss, Inc.
Markers of inflammation and risk of ovarian cancer in Los Angeles County
Authors: Wu Anna H. , Pearce Celeste L. , Tseng Chiu‐Chen , Templeman Claire , Pike Malcolm C. 
Published: October 2008

AbstractFactors that increase inflammation have been suggested to influence the development of ovarian cancer, but these factors have not been well studied. To further investigate this question, we studied the role of talc use, history of endometrioisis and use of non‐steroidal anti‐inflammatory drugs (NSAIDs) and risk of ovarian cancer in a population‐based case‐control study in Los Angeles County involving 609 women with newly diagnosed epithelial ovarian cancer and 688 population‐based control women. Risk of ovarian cancer increased significantly with increasing frequency and duration of talc use; compared to never users risk was highest among long‐duration (20+ years), frequent (at least daily) talc users (adjusted relative risk (RR) = 2.08, 95% confidence interval (CI) = 1.34–3.23). A history of physician‐diagnosed endometriosis was statistically significantly associated with risk (RR = 1.66, 95% CI = 1.01–2.75). Women who were talc users and had a history of endometriosis showed a 3‐fold increased risk (RR = 3.12, 95% CI = 1.36–7.22). Contrary to the hypothesis that risk of ovarian cancer may be reduced by use of NSAIDs; risk increased with increasing frequency (per 7 times/week, RR = 1.27, 95% CI = 1.14–1.43) and years of NSAID use (per 5 years of use, RR = 1.25, 95% CI = 1.10–1.42); this was consistent across types of NSAIDs. We conclude that risk of ovarian cancer is significantly associated with talc use and with a history of endometriosis, as has been found in previous studies. The NSAID finding was unexpected and suggests that factors associated with inflammation are associated with ovarian cancer risk. This result needs confirmation with careful attention to the reasons for NSAID use. © 2008 Wiley‐Liss, Inc.
Effects of Helicobacter pylori infection on genetic instability, the aberrant CpG island methylation status and the cellular phenotype in Barrett's esophagus in a Japanese population
Authors: Moriichi Kentaro , Watari Jiro , Das Kiron M. , Tanabe Hiroki , Fujiya Mikihiro , Ashida Toshifumi , Kohgo Yutaka 
Published: October 2008

AbstractGenetic or epigenetic alterations in Barrett's esophagus (BE) with/without Helicobacter pylori (H. pylori) infection remain unclear. We examined the effects of H. pylori infection on genetic instability (GIN), the CpG island methylation status and a biomarker related to BE carcinogenesis. We analyzed 113 Japanese individuals with endoscopically suspected BE. The patients included, Group CLE (n = 25): no specialized intestinal metaplasia (SIM) in a columnar lined epithelium (control); Group BE (n = 88): all had SIM. Microsatellite instability and a loss of heterozygosity as GIN, the methylation status at hMLH1, E‐cadherin, p16 and APC, and immunoreactivity using a monoclonal antibody (mAb) Das‐1, which specifically reacts with BE, were evaluated. Nine additional patients with BE were prospectively followed up for 2 years after successful H. pylori eradication. The frequency of GIN, methylation at E‐cadherin and APC, and mAb Das‐1 reactivity in Group BE was significantly higher than that in Group CLE (p < 0.0001, p < 0.0001 and p < 0.005, and p < 0.0001, respectively). Furthermore, GIN, E‐cadherin methylation and mAb Das‐1 reactivity showed a significantly higher incidence in patients with H.pylori infection than in those without H. pylori infection (p < 0.01, p < 0.005, and p < 0.01, respectively). Interestingly, the patients from Group BE were observed to change to a stable state of molecular alterations in 60% for GIN, 42.9% for E‐cadherin methylation and 55.6% for APC methylation, or a reduction of mAb Das‐1 reactivity was noted in 25% following eradication. H. pylori infection may therefore affect these molecular alterations associated with the pathogenesis of BE, to some degree, in the Japanese population. © 2008 Wiley‐Liss, Inc.
Radiation‐induced cathepsin S is involved in radioresistance
Authors: Seo Haeng Ran , Bae Sangwoo , Lee Yun‐Sil 
Published: October 2008

AbstractPrevious studies have suggested that the production of cathepsin S (CatS), a cysteine protease, was specifically induced in radiation‐induced rat mammary tumors. In this study, we further investigate the mechanism by which CatS is induced by radiation and its function. Radiation induced production of CatS at both the mRNA and protein level, and increased its protease activity. In addition, these radiation induced changes occurred in a dose and time‐dependent fashion. Agents such as bleomycin, As2O3 and H2O2, which produce reactive oxygen species (ROS), also induced CatS expression; however, other agents that damage DNA such as taxol and cisplatin did not. Additionally, treatment of the cells with the ROS scavengers, N‐acetylcysteine and catalase, inhibited the radiation induced CatS expression. Furthermore, radiation‐induced ROS was also involved in IFN‐γ production, which was responsible for radiation‐mediated CatS expression. Moreover, electrophoretic mobility shift assay (EMSA) data obtained using an IFN‐stimulated response element (ISRE) oligonucleotide revealed that IFN regulatory factor‐1 (IRF1) was the critical transcriptional mediator of IFN‐γ‐dependent CatS production after radiation. Finally, CatS overespression was found to induce radioresistance; however, knockdown of CatS resulted in the suppression of radioresistance. Taken together, the results of this study indicate that radiation induced CatS expression via ROS‐IFN‐γ pathways, and that this increased expression may be involved in radioresistance. © 2008 Wiley‐Liss, Inc.
Sense of coherence moderates late effects of early childhood Holocaust exposure
Authors: van der Hal‐van Raalte Elisheva A.M. , van IJzendoorn Marinus H. , Bakermans–Kranenburg Marian J. 
Published: October 2008

AbstractThis study evaluated child Holocaust survivors with an emphasis on potential protective factors facilitating participants' adaptation to post‐Holocaust life. We examined Antonovsky's (1979, 1987) salutogenic paradigm, testing the mediating and moderating effect of participants' sense of coherence (SOC) on the association between early childhood deprivation due to Holocaust persecution and posttraumatic stress later in life. The nonclinical sample, composed of 203 child Holocaust survivors born between 1935 and 1944 completed questionnaires on Holocaust survival exposure, inventories on current health, posttraumatic stress, and SOC. The results indicated that SOC moderates the association between traumatic experiences during the war and posttraumatic stress, and SOC acts as a protective factor, buffering the impact of traumatic Holocaust experiences on child survivors in old age. Survivors with a less coherent perspective on the meaning of their life showed greater vulnerability for posttraumatic complaints. The moderating role of the SOC may suggest promising avenues of therapeutic interventions for child Holocaust survivors and other adults with early childhood trauma. © 2008 Wiley Periodicals, Inc. J Clin Psychol 64:1–17, 2008.
The use of integrated countercurrent nanofiltration cascades for advanced separations
Authors: Caus Alexander , Braeken Leen , Boussu Katleen , Van der Bruggen Bart 
Published: October 2008

AbstractBACKGROUND: The concept of integrated countercurrent nanofiltration cascades is derived from fractional distillation and results in an enriched and a depleted stream, the volume and purity of which can be controlled by changing the operating parameters. The aim of this study was to assess the potential of integrated countercurrent nanofiltration cascades for advanced separation of individual organic components in aqueous solution by means of single‐stage filtration experiments using xylose and maltose and cascade simulations. The influence of module recovery, membrane characteristics, recycled fraction and the number of modules was evaluated.RESULTS: It was found that, in order to obtain a high product purity with a limited number of modules, there has to be a sufficiently high rejection difference between the components. Membranes with high rejections for the studied components (e.g. NF70 and UTC20) allow one to obtain a good separation factor but a low product recovery in a cascade configuration. A higher product recovery but lower separation factors are obtained for a cascade consisting of membranes with low rejections (e.g. NFPES10). Independently of the difference in rejection between the components, the number of modules, the recycled fraction and the module recovery determine the separation to a large extent.CONCLUSION: The optimal number of modules depends on the trade‐off between a high selectivity and a high xylose recovery; the module recovery should be as high as (practically) possible. Higher selectivities and higher product recoveries are also obtained when the retentate is recycled to the previous module. This is different from the traditional cascade approach where retentates are recycled to the next module. Copyright © 2008 Society of Chemical Industry
The skeletal kinematics of lung ventilation in three basal bird taxa (emu, tinamou, and guinea fowl)
Authors: Claessens Leon P.A.M. 
Published: October 2008

AbstractIn vivo visceral and skeletal kinematics of lung ventilation was examined using cineradiography in two palaeognaths, the emu (Dromaius novaehollandiae) and the Chilean tinamou (Nothoprocta perdicaria), and a basal neognath, the helmeted guinea fowl (Numida meleagris). Upon inspiration, the thorax expands in all dimensions. The vertebral ribs swing forward and upward, thereby increasing the transverse diameter of the trunk. The consistent location of the parapophysis throughout the dorsal vertebral series, ventral and cranial to the diapophysis, ensures a relatively uniform lateral expansion. An increase in the angle between the vertebral and the sternal ribs causes the sternal ribs to push the sternum ventrally. Owing to the greater length of the caudal sternal ribs, the caudal sternal margin is displaced further ventrally than the cranial sternal margin. When observed in lateral view, sternal movement is not linear, but elliptical.The avian thorax is highly constrained in its movement when compared with crocodylians, the other extant archosaur clade. Birds lack a lumbar region and intermediate ribs. Sternal ribs are completely ossified, and have a bicondylar articulation with the sternum. Considering the importance of pressure differences between cranial and caudal air sac complexes for the generation of unidirectional air flow in the avian lung, it is hypothesized that a decrease in the degrees of freedom of movement of the avian trunk skeleton, greater expansion of the ventrocaudal trunk region, and elliptical sternal movement may represent specific adaptations for fine‐tuned control over air flow within the complex avian pulmonary system. J. Exp. Zool. 311A:586–599, 2009. © 2008 Wiley‐Liss, Inc.