bluuebunny/crossref_metadata_2025_split huggingface dataset:
OPTIMALISASI KINERJA KARYAWAN : DAMPAK DESAIN PEKERJAAN DI PT SUMBER ALAM MUBARAK
Authors: Paroli Paroli 
Published: April 2024

Penelitian ini bertujuan untuk menginvestigasi pengaruh desain pekerjaan terhadap kinerja pegawai di PT Sumber Alam Mubarak. Metode penelitian yang digunakan adalah analisis koefisien korelasi Spearman dan uji t-statistik. Sampel penelitian melibatkan 34 responden dari berbagai departemen dalam perusahaan. Hasil penelitian menunjukkan bahwa terdapat hubungan yang kuat antara desain kerja dan kinerja pegawai dengan nilai koefisien korelasi sebesar 0,721. Uji t-statistik menegaskan bahwa desain kerja memiliki pengaruh signifikan terhadap kinerja pegawai (t hitung = 4,081, sig = 0,000). Kontribusi desain kerja terhadap kinerja pegawai sebesar 51,9%, sementara faktor lain yang tidak diteliti berkontribusi sebesar 48,1%. Kesimpulan ini konsisten dengan temuan penelitian sebelumnya. Rekomendasi melibatkan peningkatan perhatian perusahaan terhadap desain kerja sebagai strategi untuk meningkatkan kinerja pegawai. Program pelatihan dan pengembangan dapat diarahkan untuk meningkatkan pemahaman dan keterampilan terkait desain pekerjaan yang optimal. Penelitian ini memberikan kontribusi penting dalam pemahaman tentang pentingnya desain kerja dalam meningkatkan kinerja pegawai dan dapat dijadikan pedoman bagi perusahaan dalam upaya peningkatan produktivitas dan pencapaian tujuan organisasi.
Kata kunci : Desain Pekerjaan, Kinerja Pegawai

ABSTRACT
This research aims to investigate the influence of job design on employee performance at PT Sumber Alam Mubarak. The research method used was Spearman correlation coefficient analysis and t-statistical test. The research sample involved 34 respondents from various departments within the company. The research results show that there is a strong relationship between work design and employee performance with a correlation coefficient of 0.721. The t-statistical test confirms that work design has a significant influence on employee performance (t = 4.081, sig = 0.000). The contribution of work design to employee performance was 51.9%, while other factors not studied contributed 48.1%. This conclusion is consistent with previous research findings. Recommendations involve increasing corporate attention to work design as a strategy to improve employee performance. Training and development programs can be directed at increasing understanding and skills related to optimal job design. This research provides an important contribution to understanding the importance of work design in improving employee performance and can be used as a guide for companies in efforts to increase productivity and achieve organizational goals.
Symbolism in the 19th Century, its Projections on the Age of Psychology of Unconscious and Musical Aspects of That Trend
Authors: Shevchenko Liliya , Kolesnyk Olena 
Published: April 2024

The purpose of the research is to identify proto-symbolist features in the legacy of T. Shevchenko, post-symbolist indicators in the 20th century and their musical expression. The methodological basis of the research is the intonation approach in the vision of the genetic unity of music and speech principles in the development of the concepts of B. Asafiev and B. Yavorskyi in the works of D. Androsova, O. Markova, I. Podobas, L. Stepanova, L. Shevchenko, with an emphasis on the hermeneutical perspective of this approach. The methods used are analytical and structural, focusing on the musicological specifics of thematic and constructive features of the compositions' presentation, and interspecies stylistic comparative to understand the interactions of artistic, scientific and religious tendencies in the thinking of representatives of proto-symbolism, symbolism as such and post-symbolism. The scientific novelty is the emphasis of the religious and cultural scope of symbolism, in which the artistic components are subject to the general cultural requirements of creativity, given the mystical charge of expressiveness derived from icon painting and old church music, which nourished the beginnings of this trend not from the capital formations, but from those that met the needs of the religious community of the state and the nation. In this regard, the centring of symbolism in Ukraine around Odesa, which in the 19th century was the imperial centre of publishing spiritual literature, primarily Orthodox, is organic. Conclusions. Symbolism appears in art in the phenomena of proto-symbolism of the late 18th and early 19th centuries, and is expressed in its methodological independence in the second half of the 19th and early 20th century, demonstrating post-symbolist manifestations in both modern avant-garde and traditionalism, as demonstrated in Ukraine by the compositions of B. Liatoshynskyi and V. Kosenko, for whom, like for many prominent artists, the appeal to the symbolist origins was not only a conscious act, but also a subconscious appeal in search of metaphorical saturation of artistic expression, which provokes the creation of symbolist "supercomplex" metaphorical images.
From creating conditions for forming functional literacy of students to achievement of planned results in the context of the federal state educational standard of basic general education
Authors: Riefling A. A. , Belous S. O. , Korolev Yu. V. , Chernova O. N. , Zhukova V. N. , Shchukina I. A. 
Published: April 2024

The article presents the diagnostic work to determine the level of functional literacy in students of 7th–8th grades, including reading, natural science and mathematical literacy. Assessment of functional literacy is aimed at identifying the conditions for successful learning of students in basic school in the subject “Informatics”. The peculiarity of tasks for the development of functional literacy is that the problems and tasks in them are described using the example of life situations and are initially posed outside a specific subject area.The relevance of the article is due to the fact that all-Russian testing is not carried out in the subject “Informatics” — monitoring the quality of students’ training, including checking the development of functional literacy, therefore this diagnostic work will be in demand by informatics teachers.The diagnostic work can be used to monitor the formation of universal educational activities in working with information and reading, to test the ability to apply basic mathematical and natural science knowledge in situations of a practice-oriented nature, as well as the ability to conduct research.One variant is used for diagnostics. It includes an introductory text explaining the situation and tasks for it, built on the context of educational material in various subject areas (informatics, mathematics, natural science).
A pénzügyi fogyasztóvédelem aktuális kérdései, kihívások a 2020-as években
Authors: Vértesy László 
Published: April 2024

Az elmúlt évtizedek során a pénzügyi fogyasztóvédelem számottevő fejlődésen ment keresztül, a 2020-as évek főbb kérdései két nagyobb csoportra oszthatók. Az egyik a hatósági, felügyeleti jogalkalmazás erősítése: a nemzeti és nemzetközi fokozott jogérvényesítés, a visszaesőkre való összpontosítás, a tisztességes hitelezés, a díjpolitikák és gyakorlatok széles körű vizsgálata, fogyasztói panaszok, jogviták figyelemmel kísérése és fokozott koordináció a szabályozó hatóságok között. A másik pedig a technológiai fejlődéssel való lépéstartáshoz kapcsolódik, úgymint a fintech vállalatok fejlődése, a neobanking, a kriptovaluták, a fenntarthatóságot hangsúlyozó ESG, öko, zéró és a zöld technológiát finanszírozó termékek elterjedése, valamint a kiberbiztonság és az elektronikus adatvédelem. Az USA jó gyakorlatokkal szolgál a pénzügyi fogyasztóvédelem terén, erősítve a hatóságok szakértelmét a gyorsan változó pénzügyi környezetben. A Magyar Nemzeti Bank is aktívan részt vesz a pénzügyi fogyasztóvédelem és a pénzügyi stabilitás előmozdításában.
Qualitative and quantitative chemical analysis of Leptadenia hastata: exploring a traditional african medicinal plant
Authors: Zhang Jiangsheng , Nan Yi , Su Jie , Jibril Aminu Usman , Lv Guiyuan 
Published: April 2024

Leptadenia hastata (Pers.) Decne is a commonly used food source and prescribed as a traditional African medicine for treatment of various diseases, such as diabetes, skin disorders, wounds, and ulcers. However, quality control has become a bottleneck restricting the therapeutic development and utilization of this plant. In this study, a reliable method for qualitative and quantitative determination of components in Leptadenia hastata was established. The components of L. hastata were profiled using ultra-high performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS). Subsequently, an ultra-high performance tandem diode array detector (UHPLC-DAD)-based method was used for simultaneous quantitative analysis of five major constituents in six batches of L. hastata samples. As a result, 35 compounds were tentatively identified. The quantities of the five constituents (vicenin-Ⅱ, orientin, schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, chrysin 6-C-glucoside 8-C-arabinoside) were determined as 124.8–156.9 μg/g, 170.5–216.0 μg/g, 61.31–93.73 μg/g, 85.13–119.3 μg/g and 99.82–129.4 μg/g, respectively. This method offers a successful strategy for precise and effective evaluation of the constituents of L. hastata, providing a robust foundation for holistic quality assessment of medicinal plants.
Yeast species in the respiratory samples of COVID-19 patients; molecular tracking of Candida auris
Authors: Rouhi Faezeh , Soltani Sajedeh , Sadeghi Somayeh , Nasri Elahe , Hosseini Mahnaz , Ghafel Safiyeh , Aboutalebian Shima , Fakhim Hamed , Mirhendi Hossein 
Published: April 2024

IntroductionAlthough the existence of Candida species in the respiratory tract is often considered commensal, it is crucial to recognize the significance of Candida colonization in immunocompromised or COVID-19 patients. The emergence of Candida auris as an emerging pathogen further emphasizes the importance of monitoring yeast infection/colonization, particularly in COVID-19 patients.MethodsIn this study, respiratory samples mainly from COVID-19 patients, primarily those suspected of having a fungal infection, were cultured on Sabouraud dextrose agar plates and the yeast colonies were identified using a two-step multiplex PCR method. The samples suspected of C. auris underwent specific nested PCR followed by sequence analysis.ResultsA total of 199 respiratory samples were collected from 73 women and 126 men, ranging in age from 1.6 to 88 years. Among the patients, 141 had COVID-19, 32 had cancer, 5 were hospitalized in ICU, 2 had chronic obstructive pulmonary disease)COPD(, and others were patients with combination diseases. From these samples, a total of 334 yeast strains were identified. C. albicans (n=132, 39.52%) was the most common species, followed by C. tropicalis (n=67, 20%), C. glabrata (n=56, 16.76%), C. krusei (n=18, 5.4%), C. parapsilosis (n=17, 5.08%), Saccharomyces cerevisiae (n=10, 3%), C. kefyr (n=9, 2.6%), C. dubliniensis (n=7, 2.1%), C. lusitaniae (n=5, 1.5%), C. auris (n=3, 0.9%), C. guilliermondii (n=2, 0.6%), C. rugosa (n=1, 0.3%), C. intermedia (n=1, 0.3%), and Trichosporon spp. (n=1, 0.3%). C. auris was detected in a patient in ICU and two COVID-19 patients. While its presence was confirmed through sequence analysis, our extensive efforts to isolate C. auris were unsuccessful.ConclusionWhile C. albicans colonization remains prevalent, our study found no evidence of Candida lung infection. Since the role of Candida colonization in airway secretions remains ambiguous due to limited research, further studies are imperative to shed light on this matter.
Uncovering specific taxonomic and functional alteration of gut microbiota in chronic kidney disease through 16S rRNA data
Authors: Zhang Yangyang , Zhong Weicong , Liu Wenting , Wang Xiaohua , Lin Gan , Lin Jiawen , Fang Junxuan , Mou Xiangyu , Jiang Shan , Huang Jiayuan , Zhao Wenjing , Zheng Zhihua 
Published: April 2024

IntroductionChronic kidney disease (CKD) is worldwide healthcare burden with growing incidence and death rate. Emerging evidence demonstrated the compositional and functional differences of gut microbiota in patients with CKD. As such, gut microbial features can be developed as diagnostic biomarkers and potential therapeutic target for CKD.MethodsTo eliminate the outcome bias arising from factors such as geographical distribution, sequencing platform, and data analysis techniques, we conducted a comprehensive analysis of the microbial differences between patients with CKD and healthy individuals based on multiple samples worldwide. A total of 980 samples from six references across three nations were incorporated from the PubMed, Web of Science, and GMrepo databases. The obtained 16S rRNA microbiome data were subjected to DADA2 processing, QIIME2 and PICRUSt2 analyses.ResultsThe gut microbiota of patients with CKD differs significantly from that of healthy controls (HC), with a substantial decrease in the microbial diversity among the CKD group. Moreover, a significantly reduced abundance of bacteria Faecalibacterium prausnitzii (F. prausnitzii) was detected in the CKD group through linear discriminant analysis effect size (LEfSe) analysis, which may be associated with the alleviating effects against CKD. Notably, we identified CKD-depleted F. prausnitzii demonstrated a significant negative correlation with three pathways based on predictive functional analysis, suggesting its potential role in regulating systemic acidbase disturbance and pro-oxidant metabolism.DiscussionOur findings demonstrated notable alterations of gut microbiota in CKD patients. Specific gut-beneficial microbiota, especially F. prausnitzii, may be developed as a preventive and therapeutic tool for CKD clinical management.
Transcriptional profiles associated with coronary artery disease in type 2 diabetes mellitus
Authors: Nevado Jose B. , Cutiongco-de la Paz Eva Maria C. , Paz-Pacheco Elizabeth T. , Jasul Gabriel V. , Aman Aimee Yvonne Criselle L. , Deguit Christian Deo T. , San Pedro Jana Victoria B. , Francisco Mark David G. 
Published: April 2024

BackgroundCoronary artery disease (CAD) is a common complication of Type 2 diabetes mellitus (T2DM). Understanding the pathogenesis of this complication is essential in both diagnosis and management. Thus, this study aimed to characterize the presence of CAD in T2DM using molecular markers and pathway analyses.MethodsThe study is a sex- and age-frequency matched case-control design comparing 23 unrelated adult Filipinos with T2DM-CAD to 23 controls (DM with CAD). Healthy controls served as a reference. Total RNA from peripheral blood mononuclear cells (PBMCs) underwent whole transcriptomic profiling using the Illumina HumanHT-12 v4.0 expression beadchip. Differential gene expression with gene ontogeny analyses was performed, with supporting correlational analyses using weighted correlation network analysis (WGCNA).ResultsThe study observed that 458 genes were differentially expressed between T2DM with and without CAD (FDR&lt;0.05). The 5 top genes the transcription factor 3 (TCF3), allograft inflammatory factor 1 (AIF1), nuclear factor, interleukin 3 regulated (NFIL3), paired immunoglobulin-like type 2 receptor alpha (PILRA), and cytoskeleton-associated protein 4 (CKAP4) with AUCs &gt;89%. Pathway analyses show differences in innate immunity activity, which centers on the myelocytic (neutrophilic/monocytic) theme. SNP-module analyses point to a possible causal dysfunction in innate immunity that triggers the CAD injury in T2DM.ConclusionThe study findings indicate the involvement of innate immunity in the development of T2DM-CAD, and potential immunity markers can reflect the occurrence of this injury. Further studies can verify the mechanistic hypothesis and use of the markers.
Dissecting the shared genetic architecture between Alzheimer’s disease and frailty: a cross-trait meta-analyses of genome-wide association studies
Authors: Enduru Nitesh , Fernandes Brisa S. , Zhao Zhongming 
Published: April 2024

Introduction: Frailty is the most common medical condition affecting the aging population, and its prevalence increases in the population aged 65 or more. Frailty is commonly diagnosed using the frailty index (FI) or frailty phenotype (FP) assessments. Observational studies have indicated the association of frailty with Alzheimer’s disease (AD). However, the shared genetic and biological mechanism of these comorbidity has not been studied.Methods: To assess the genetic relationship between AD and frailty, we examined it at single nucleotide polymorphism (SNP), gene, and pathway levels.Results: Overall, 16 genome-wide significant loci (15 unique loci) (pmeta-analysis &lt; 5 × 10−8) and 22 genes (21 unique genes) were identified between AD and frailty using cross-trait meta-analysis. The 8 shared loci implicated 11 genes: CLRN1-AS1, CRHR1, FERMT2, GRK4, LINC01929, LRFN2, MADD, RP11-368P15.1, RP11-166N6.2, RNA5SP459, and ZNF652 between AD and FI, and 8 shared loci between AD and FFS implicated 11 genes: AFF3, C1QTNF4, CLEC16A, FAM180B, FBXL19, GRK4, LINC01104, MAD1L1, RGS12, ZDHHC5, and ZNF521. The loci 4p16.3 (GRK4) was identified in both meta-analyses. The colocalization analysis supported the results of our meta-analysis in these loci. The gene-based analysis revealed 80 genes between AD and frailty, and 4 genes were initially identified in our meta-analyses: C1QTNF4, CRHR1, MAD1L1, and RGS12. The pathway analysis showed enrichment for lipoprotein particle plasma, amyloid fibril formation, protein kinase regulator, and tau protein binding.Conclusion: Overall, our results provide new insights into the genetics of AD and frailty, suggesting the existence of non-causal shared genetic mechanisms between these conditions.
Use of whole-genome sequence data for fine mapping and genomic prediction of sea louse resistance in Atlantic salmon
Authors: Onabanjo Olumide , Meuwissen Theo , Aslam Muhammad Luqman , Schmitt Armin Otto , Dagnachew Binyam 
Published: April 2024

Sea louse (Lepeophtheirus salmonis) infestation of Atlantic salmon (Salmo salar) is a significant challenge in aquaculture. Over the years, this parasite has developed immunity to medicinal control compounds, and non-medicinal control methods have been proven to be stressful, hence the need to study the genomic architecture of salmon resistance to sea lice. Thus, this research used whole-genome sequence (WGS) data to study the genetic basis of the trait since most research using fewer SNPs did not identify significant quantitative trait loci. Mowi Genetics AS provided the genotype (50 k SNPs) and phenotype data for this research after conducting a sea lice challenge test on 3,185 salmon smolts belonging to 191 full-sib families. The 50 k SNP genotype was imputed to WGS using the information from 197 closely related individuals with sequence data. The WGS and 50 k SNPs of the challenged population were then used to estimate genetic parameters, perform a genome-wide association study (GWAS), predict genomic breeding values, and estimate its accuracy for host resistance to sea lice. The heritability of host resistance to sea lice was estimated to be 0.21 and 0.22, while the accuracy of genomic prediction was estimated to be 0.65 and 0.64 for array and WGS data, respectively. In addition, the association test using both array and WGS data did not identify any marker associated with sea lice resistance at the genome-wide level. We conclude that sea lice resistance is a polygenic trait that is moderately heritable. The genomic predictions using medium-density SNP genotyping array were equally good or better than those based on WGS data.
A novel cis-regulatory element regulates αD and αA-globin gene expression in chicken erythroid cells
Authors: Cortés-Fernández de Lara Josué , Núñez-Martínez Hober Nelson , Tapia-Urzúa Gustavo , Garza-Manero Sylvia , Peralta-Alvarez Carlos Alberto , Furlan-Magaril Mayra , González-Buendía Edgar , Escamilla-Del-Arenal Martín , Casasola Andrea , Guerrero Georgina , Recillas-Targa Felix 
Published: April 2024

BackgroundCis-regulatory elements (CREs) play crucial roles in regulating gene expression during erythroid cell differentiation. Genome-wide erythroid-specific CREs have not been characterized in chicken erythroid cells, which is an organism model used to study epigenetic regulation during erythropoiesis.MethodsAnalysis of public genome-wide accessibility (ATAC-seq) maps, along with transcription factor (TF) motif analysis, CTCF, and RNA Pol II occupancy, as well as transcriptome analysis in fibroblasts and erythroid HD3 cells, were used to characterize erythroid-specific CREs. An α-globin CRE was identified, and its regulatory activity was validated in vitro and in vivo by luciferase activity and genome-editing assays in HD3 cells, respectively. Additionally, circular chromosome conformation capture (UMI-4C) assays were used to distinguish its role in structuring the α-globin domain in erythroid chicken cells.ResultsErythroid-specific CREs displayed occupancy by erythroid TF binding motifs, CTCF, and RNA Pol II, as well as an association with genes involved in hematopoiesis and cell differentiation. An α-globin CRE, referred to as CRE-2, was identified as exhibiting enhancer activity over αD and αA genes in vitro and in vivo. Induction of terminal erythroid differentiation showed that α-globin CRE-2 is required for the induction of αD and αA. Analysis of TF binding motifs at α-globin CRE-2 shows apparent regulation mediated by GATA-1, YY1, and CTCF binding.ConclusionOur findings demonstrate that cell-specific CREs constitute a key mechanism that contributes to the fine-tuning gene regulation of erythroid cell differentiation and provide insights into the annotation and characterization of CREs in chicken cells.
The non-pathogenic protozoon Leishmania tarentolae interferes with the activation of NLRP3 inflammasome in human cells: new perspectives in the control of inflammation
Authors: La Rosa Francesca , Varotto-Boccazzi Ilaria , Saresella Marina , Marventano Ivana , Cattaneo Giulia Maria , Hernis Ambra , Piancone Federica , Otranto Domenico , Epis Sara , Bandi Claudio , Clerici Mario 
Published: April 2024

BackgroundInnate immune responses against infectious agents can act as triggers of inflammatory diseases. On the other hand, various pathogens have developed mechanisms for the evasion of the immune response, based on an inhibition of innate immunity and inflammatory responses. Inflammatory diseases could thus be controlled through the administration of pathogens or pathogen-derived molecules, capable of interfering with the mechanisms at the basis of inflammation. In this framework, the NLRP3 inflammasome is an important component in innate antimicrobial responses and a major player in the inflammatory disease. Parasites of the genus Leishmania are master manipulators of innate immune mechanisms, and different species have been shown to inhibit inflammasome formation. However, the exploitation of pathogenic Leishmania species as blockers of NLRP3-based inflammatory diseases poses safety concerns.MethodsTo circumvent safety issues associated with pathogenic parasites, we focused on Leishmania tarentolae, a species of Leishmania that is not infectious to humans. Because NLRP3 typically develops in macrophages, in response to the detection and engulfment microorganisms, we performed our experiments on a monocyte-macrophage cell line (THP-1), either wild type or knockout for ASC, a key component of NLRP3 formation, with determination of cytokines and other markers of inflammation.ResultsL. tarentolae was shown to possess the capability of dampening the formation of NLRP3 inflammasome and the consequent expression of pro-inflammatory molecules, with minor differences compared to effects of pathogenic Leishmania species.ConclusionThe non-pathogenic L. tarentolae appears a promising pro-biotic microbe with anti-inflammatory properties or a source of immune modulating cellular fractions or molecules, capable of interfering with the formation of the NLRP3 inflammasome.