bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the NF-kB signalling pathway in heterotopic ossification: biological and therapeutic significance
Authors: Liu Fangzhou , Zhao Yike , Pei Yiran , Lian Fengyu , Lin Hui 
Published: March 2024

AbstractHeterotopic ossification (HO) is a pathological process in which ectopic bone develops in soft tissues within the skeletal system. Endochondral ossification can be divided into the following types of acquired and inherited ossification: traumatic HO (tHO) and fibrodysplasia ossificans progressiva (FOP). Nuclear transcription factor kappa B (NF-κB) signalling is essential during HO. NF-κB signalling can drive initial inflammation through interactions with the NOD‐like receptor protein 3 (NLRP3) inflammasome, Sirtuin 1 (SIRT1) and AMP-activated protein kinase (AMPK). In the chondrogenesis stage, NF-κB signalling can promote chondrogenesis through interactions with mechanistic target of rapamycin (mTOR), phosphatidylinositol-3-kinase (PI3K)/AKT (protein kinase B, PKB) and other molecules, including R-spondin 2 (Rspo2) and SRY-box 9 (Sox9). NF-κB expression can modulate osteoblast differentiation by upregulating secreted protein acidic and rich in cysteine (SPARC) and interacting with mTOR signalling, bone morphogenetic protein (BMP) signalling or integrin-mediated signalling under stretch stimulation in the final osteogenic stage. In FOP, mutated ACVR1-induced NF-κB signalling exacerbates inflammation in macrophages and can promote chondrogenesis and osteogenesis in mesenchymal stem cells (MSCs) through interactions with smad signalling and mTOR signalling. This review summarizes the molecular mechanism of NF-κB signalling during HO and highlights potential therapeutics for treating HO.
Mitochondrial metabolism in neural stem cells and implications for neurodevelopmental and neurodegenerative diseases
Authors: Garone C. , De Giorgio F. , Carli S. 
Published: March 2024

AbstractMitochondria are cytoplasmic organelles having a fundamental role in the regulation of neural stem cell (NSC) fate during neural development and maintenance.During embryonic and adult neurogenesis, NSCs undergo a metabolic switch from glycolytic to oxidative phosphorylation with a rise in mitochondrial DNA (mtDNA) content, changes in mitochondria shape and size, and a physiological augmentation of mitochondrial reactive oxygen species which together drive NSCs to proliferate and differentiate. Genetic and epigenetic modifications of proteins involved in cellular differentiation (Mechanistic Target of Rapamycin), proliferation (Wingless-type), and hypoxia (Mitogen-activated protein kinase)–and all connected by the common key regulatory factor Hypoxia Inducible Factor-1A–are deemed to be responsible for the metabolic shift and, consequently, NSC fate in physiological and pathological conditions.Both primary mitochondrial dysfunction due to mutations in nuclear DNA or mtDNA or secondary mitochondrial dysfunction in oxidative phosphorylation (OXPHOS) metabolism, mitochondrial dynamics, and organelle interplay pathways can contribute to the development of neurodevelopmental or progressive neurodegenerative disorders.This review analyses the physiology and pathology of neural development starting from the available in vitro and in vivo models and highlights the current knowledge concerning key mitochondrial pathways involved in this process.
The genetic basis of hydrocephalus: genes, pathways, mechanisms, and global impact
Authors: Hale Andrew T. , Boudreau Hunter , Devulapalli Rishi , Duy Phan Q. , Atchley Travis J. , Dewan Michael C. , Goolam Mubeen , Fieggen Graham , Spader Heather L. , Smith Anastasia A. , Blount Jeffrey P. , Johnston James M. , Rocque Brandon G. , Rozzelle Curtis J. , Chong Zechen , Strahle Jennifer M. , Schiff Steven J. , Kahle Kristopher T. 
Published: March 2024

AbstractHydrocephalus (HC) is a heterogenous disease characterized by alterations in cerebrospinal fluid (CSF) dynamics that may cause increased intracranial pressure. HC is a component of a wide array of genetic syndromes as well as a secondary consequence of brain injury (intraventricular hemorrhage (IVH), infection, etc.) that can present across the age spectrum, highlighting the phenotypic heterogeneity of the disease. Surgical treatments include ventricular shunting and endoscopic third ventriculostomy with or without choroid plexus cauterization, both of which are prone to failure, and no effective pharmacologic treatments for HC have been developed. Thus, there is an urgent need to understand the genetic architecture and molecular pathogenesis of HC. Without this knowledge, the development of preventive, diagnostic, and therapeutic measures is impeded. However, the genetics of HC is extraordinarily complex, based on studies of varying size, scope, and rigor. This review serves to provide a comprehensive overview of genes, pathways, mechanisms, and global impact of genetics contributing to all etiologies of HC in humans.
scAbsolute: measuring single-cell ploidy and replication status
Authors: Schneider Michael P. , Cullen Amy E. , Pangonyte Justina , Skelton Jason , Major Harvey , Van Oudenhove Elke , Garcia Maria J. , Chaves Urbano Blas , Piskorz Anna M. , Brenton James D. , Macintyre Geoff , Markowetz Florian 
Published: March 2024

AbstractCancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy. Correct estimation of the ploidy of single-cell genomes is paramount for downstream analysis. Based only on single-cell DNA sequencing information, scAbsolute achieves accurate and unbiased measurement of single-cell ploidy and replication status, including whole-genome duplications. We demonstrate scAbsolute’s capabilities using experimental cell multiplets, a FUCCI cell cycle expression system, and a benchmark against state-of-the-art methods. scAbsolute provides a robust foundation for single-cell DNA sequencing analysis across different technologies and has the potential to enable improvements in a number of downstream analyses.
Vaccination and the risk of systemic lupus erythematosus: a meta-analysis of observational studies
Authors: Wang Meijiao , Gu Huanpeng , Zhai Yingqi , Li Xuanlin , Huang Lin , Li Haichang , Xie Zhijun , Wen Chengping 
Published: March 2024

Abstract
Objective
This meta-analysis aims to explore the potential link between vaccines and systemic lupus erythematosus (SLE).

Methods
We systematically searched PubMed, Cochrane Library, and Embase for observational studies from inception to September 3, 2023, using medical subject headings (MeSH) and keywords. Study quality was assessed using the NOS scale. Statistical analyses were conducted using STATA software (version 14.0). Publication bias was evaluated using funnel plots and Egger’s regression.

Results
The meta-analysis incorporated 17 studies, encompassing 45,067,349 individuals with follow-up periods ranging from 0.5 to 2 years. The pooled analysis revealed no significant association between vaccinations and an increased risk of SLE [OR = 1.14, 95% CI (0.86–1.52), I2 = 78.1%, P = 0.348]. Subgroup analyses indicated that HBV vaccination was significantly associated with an elevated risk of SLE [OR =2.11, 95% CI (1.11-4.00), I2 = 63.3%, P = 0.02], HPV vaccination was slightly associated with an increased risk of SLE [OR = 1.43, 95% CI (0.88–2.31), I2 = 72.4%, P = 0.148], influenza vaccination showed no association with an increased risk of SLE [OR = 0.96, 95% CI (0.82–1.12), I2 = 0.0%, P = 0.559], and COVID-19 vaccine was marginally associated with a decreased risk of SLE [OR = 0.44, 95% CI (0.18–1.21), I2 = 91.3%, P = 0.118].

Conclusions
This study suggests that vaccinations are not linked to an increased risk of SLE. Our meta-analysis results provide valuable insights, alleviating concerns about SLE risk post-vaccination and supporting further vaccine development efforts.
Prognostic significance of the stress hyperglycemia ratio and admission blood glucose in diabetic and nondiabetic patients with spontaneous intracerebral hemorrhage
Authors: Liang Shengru , Tian Xiaoxi , Gao Fei , Man Minghao , Wang Qi , Li Jianwei , Li Lihong , Yang Yang 
Published: March 2024

Abstract
Background
The role of stress hyperglycemia ratio (SHR) on the prognosis of spontaneous intracerebral hemorrhage (ICH) in patients with different diabetic status has not been elucidated. This study aimed to evaluate the prognostic value of SHR and admission blood glucose (ABG) for the short- and long-term mortality in diabetic and nondiabetic populations with ICH.

Method
Participants with ICH were retrospectively retrieved from the Medical Information Mart for Intensive Care (MIMIC-IV). The primary outcome was all-cause 30-day and 1-year mortality. The association of SHR and ABG with the primary outcomes in diabetic and nondiabetic cohorts were assessed by Cox proportional hazard regression.

Results
Overall, 1029 patients with a median age of 71.09 (IQR: 60.05–81.97) were included. Among them, 548 (53%) individuals were male, and 95 (19%) as well as 323 (31%) ones experienced the 30-day and 1-year mortality, respectively. After adjusting for confounding variables, individuals in quintile 5 of SHR had significantly higher risk of the 30-day and 1-year mortality than those in quintile 1 in the whole cohort (30-day mortality: HR 3.33, 95%CI 2.01–5.51; 1-year mortality: HR 2.09, 95% CI 1.46-3.00) and in nondiabetic patients (30-day mortality: HR 4.55, 95%CI 2.33–8.88; 1-year mortality: HR 3.06, 95%CI 1.93–4.86), but no significant difference was observed in diabetic patients. Similar results were observed for ABG as a categorical variable. As continuous variable, SHR was independently correlated with the 30-day and 1-year mortality in both of the diabetic and nondiabetic cohorts (30-day mortality: HR 2.63, 95%CI 1.50–4.60. 1-year mortality: HR 2.12, 95%CI 1.33–3.39), but this correlation was only observed in nondiabetic cohort for ABG (HR 1.00, 95%CI 0.99–1.01 for both of the 30-day and 1-year mortality). Moreover, compared with ABG, SHR can better improve the C-statistics of the original models regarding the 30-day and 1-year outcomes, especially in patients with diabetes (p < 0.001 in all models).

Conclusion
SHR might be a more useful and reliable marker than ABG for prognostic prediction and risk stratification in critically ill patients with ICH, especially in those with diabetes.
The marine conservation deposits of Monte San Giorgio (Switzerland, Italy): the prototype of Triassic black shale Lagerstätten
Authors: Klug Christian , Spiekman Stephan N. F. , Bastiaans Dylan , Scheffold Beat , Scheyer Torsten M. 
Published: March 2024

AbstractMarine conservation deposits (‘Konservat-Lagerstätten’) are characterized by their mode of fossil preservation, faunal composition and sedimentary facies. Here, we review these characteristics with respect to the famous conservation deposit of the Besano Formation (formerly Grenzbitumenzone; including the Anisian–Ladinian boundary), and the successively younger fossil-bearing units Cava inferiore, Cava superiore, Cassina beds and the Kalkschieferzone of Monte San Giorgio (Switzerland and Italy). We compare these units to a selection of important black shale-type Lagerstätten of the global Phanerozoic plus the Ediacaran in order to detect commonalities in their facies, genesis, and fossil content using principal component and hierarchical cluster analyses. Further, we put the Monte San Giorgio type Fossillagerstätten into the context of other comparable Triassic deposits worldwide based on their fossil content. The results of the principal component and cluster analyses allow a subdivision of the 45 analysed Lagerstätten into four groups, for which we suggest the use of the corresponding pioneering localities: Burgess type for the early Palaeozoic black shales, Monte San Giorgio type for the Triassic black shales, Holzmaden type for the pyrite-rich black shales and Solnhofen type for platy limestones.
The learning performance of the weak rescaled pure greedy algorithms
Authors: Guo Qin , Liu Xianghua , Ye Peixin 
Published: March 2024

AbstractWe investigate the regression problem in supervised learning by means of the weak rescaled pure greedy algorithm (WRPGA). We construct learning estimator by applying the WRPGA and deduce the tight upper bounds of the K-functional error estimate for the corresponding greedy learning algorithms in Hilbert spaces. Satisfactory learning rates are obtained under two prior assumptions on the regression function. The application of the WRPGA in supervised learning considerably reduces the computational cost while maintaining its powerful generalization capability when compared with other greedy learning algorithms.
Changes in albedo and its radiative forcing of grasslands in East Asia drylands
Authors: Zhu Qingsong , Chen Jiquan , Wu Liangxu , Huang Yuting , Shao Changliang , Dong Gang , Xu Zhe , Li Xianglan 
Published: March 2024

Abstract
Background
Grasslands in drylands are increasingly influenced by human activities and climate change, leading to alterations in albedo and radiative energy balance among others. Surface biophysical properties and their interactions change greatly following disturbances. However, our understanding of these processes and their climatic impacts remains limited. In this study, we used multi-year observations from satellites and eddy-covariance towers to investigate the response of albedo to variables closely associated with human disturbances, including vegetation greenness (EVI) and surface soil volumetric water content (VWC), as well as snow cover and clearness index (Ta) for their potential relationships.

Results
EVI and VWC during the growing season were the primary factors influencing albedo. EVI and VWC were negatively correlated with albedo, with VWC’s total direct and indirect impacts being slightly smaller than those of EVI. During the non-growing season, snow cover was the most influential factor on albedo. VWC and Ta negatively affected albedo throughout the year. We estimated the impact of variations in EVI and VWC on climate to be in the range of 0.004 to 0.113 kg CO2 m−2 yr−1 in CO2 equivalent.

Conclusions
This study indicates the significant impacts of climate change and human disturbances on vulnerable grassland ecosystems from the perspective of altered albedo. Changes in vegetation greenness and soil properties induced by climate change and human activities may have a substantial impact on albedo, which in turn feedback on climate change, indicating that future climate policies should take this factor into consideration.
Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens
Authors: Fu Yu , Zhou Jianmin , Schroyen Martine , Zhang Haijun , Wu Shugeng , Qi Guanghai , Wang Jing 
Published: March 2024

Abstract
Background
Deteriorations in eggshell and bone quality are major challenges in aged laying hens. This study compared the differences of eggshell quality, bone parameters and their correlations as well as uterine physiological characteristics and the bone remodeling processes of hens laying eggs of different eggshell breaking strength to explore the mechanism of eggshell and bone quality reduction and their interaction. A total of 240 74-week-old Hy-line Brown laying hens were selected and allocated to a high (HBS, 44.83 ± 1.31 N) or low (LBS, 24.43 ± 0.57 N) eggshell breaking strength group.

Results
A decreased thickness, weight and weight ratio of eggshells were observed in the LBS, accompanied with ultrastructural deterioration and total Ca reduction. Bone quality was negatively correlated with eggshell quality, marked with enhanced structures and increased components in the LBS. In the LBS, the mammillary knobs and effective layer grew slowly. At the initiation stage of eggshell calcification, a total of 130 differentially expressed genes (DEGs, 122 upregulated and 8 downregulated) were identified in the uterus of hens in the LBS relative to those in the HBS. These DEGs were relevant to apoptosis due to the cellular Ca overload. Higher values of p62 protein level, caspase-8 activity, Bax protein expression and lower values of Bcl protein expression and Bcl/Bax ratio were seen in the LBS. TUNEL assay and hematoxylin-eosin staining showed a significant increase in TUNEL-positive cells and tissue damages in the uterus of the LBS. Although few DEGs were identified at the growth stage, similar uterine tissue damages were also observed in the LBS. The expressions of runt-related transcription factor 2 and osteocalcin were upregulated in humeri of the LBS. Enlarged diameter and more structural damages of endocortical bones and decreased ash were observed in femurs of the HBS.

Conclusion
The lower eggshell breaking strength may be attributed to a declined Ca transport due to uterine tissue damages, which could affect eggshell calcification and lead to a weak ultrastructure. Impaired uterine Ca transport may result in reduced femoral bone resorption and increased humeral bone formation to maintain a higher mineral and bone quality in the LBS.

Graphical Abstract
Post-pandemic crime trends in England and Wales
Authors: Seyidoglu Hulya , Farrell Graham , Dixon Anthony , Pina-Sánchez Jose , Malleson Nick 
Published: March 2024

AbstractThis study of recorded crime trends in England & Wales spans three and a half years, that is, two covid pandemic years from March 2020 and 18 ‘post-pandemic’ months following cessation of covid restrictions. Observed crime rates were compared to expected (based on 5-year ARIMA models) and the ambient population (using Community Mobility Reports). It finds that, In Year 1, observed rates diverged dramatically from expected, waxing and waning generally in line with the movement restrictions of three national lockdowns. In Year 2, movement restrictions loosened and observed crime rates moved towards but mostly remained far from expected. In post-pandemic Year 3, people’s movement increased and observed crime rates continued towards expected. By mid-Year 4 many rates remained below expected levels, their mean monthly differences including: theft from person (− 22%); burglary (− 20%); vehicle crime (− 29%); violence & sexual offences (− 27%); robbery (− 16%) and; public order offences (− 21%). An exceptional increase in shoplifting achieved 20% above expected rates by August 2023. Methodological limitations and further research on shoplifting and other issues are discussed. The main conclusion is that crime trends generally followed ambient population movement and that enduring lifestyle changes in the post-pandemic period, notably increased work-from-home, account for continuing below-expected rates of many crime types.
Teacher support in language learning: a picture of the effects on language progress, academic immunity, and academic enjoyment
Authors: Huang Lan , Al-Rashidi Anwar Hammad , Bayat Sania 
Published: March 2024

AbstractTeacher support encompasses the provision of educational, instrumental, sentimental, or evaluative assistance by a teacher to a student, regardless of the setting. Learning a new language brings with it different ups and downs that may hinder the actual progress of the learners. The journey of learning may be made easier by supportive instructors. During the course of this inquiry, two groups (85 participants in total) including the experimental and control groups were chosen to gauge the influence of Teacher support in virtual instruction on English as a foreign language (EFL) learners’ progress, academic immunity, and academic enjoyment. In contrast to the experimental group (EG, n = 42), which had the chance to connect with a teacher in order to ask questions and get feedback plus the off-line education, the control group (CG, n = 44) was subjected to off-line instruction of language input (Top Notch 3). The status of the participants’ language growth, immunity, and enjoyment was investigated both before and after the treatment. The findings of independent samples t-tests as well as the multivariate analysis of variance (MANOVA) indicated that learners in EG did much better than their counterparts in CG. In addition, the ramifications and recommendations for potential future directions are analyzed.
The effect of social media upward comparison on Chinese adolescent learning engagement: a moderated multiple mediation model
Authors: Qi Xinjie , Jiang Yi , Lian Rong 
Published: March 2024

AbstractTo investigate the mechanisms and boundary conditions of social media upward comparison on adolescents’ learning engagement, this study utilized two separate approaches. In research 1, a convenience sampling method was employed to conduct a questionnaire survey among 609 adolescents. The aim was to explore the influence of social media’s upward comparative tendency on learning engagement. In research 2, real social media platforms were utilized to select short videos as experimental materials. These materials were used to manipulate instantaneous social media upward comparison and examine its impact on learning engagement. The research 1’s result indicated that the mediating effect of the sense of agency was significant, as well as the moderating effect of growth mindset. However, the mediating effect of positive-negative emotions was found to be insignificant.Regarding instantaneous social media upward comparison and learning engagement, positive and negative emotions were found to play a complete mediating role. However, the mediating role of agency and the moderating role of growth mindset were not significant.This study offers practical insights for educators, enabling them to develop a correct understanding and effectively regulate adolescents’ use of social media.