bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of dissociation rate of biomolecular complexes using CE
Authors: Yang Peilin , Mao Yingwei , Lee Angel W.‐M. , Kennedy Robert T. 
Published: January 2009

AbstractFluorescence anisotropy (FA), non‐equilibrium CE of equilibrium mixtures (NECEEM) and high‐speed CE were evaluated for measuring dissociation kinetics of peptide–protein binding systems. Fyn‐SH3‐SH2, a protein construct consisting of the src homology 2 (SH2) and 3 (SH3) domain of the protein Fyn, and a fluorescein‐labeled phosphopeptide were used as a model system. All three methods gave comparable half‐life of∼53 s for Fyn‐SH3‐SH2:peptide complex. Achieving satisfactory results by NECEEM required columns over 30 cm long. When using Fyn‐SH2‐SH3 tagged with glutathione S‐transferase (GST) as the binding protein, both FA and NECEEM assays gave evidence of two complexes forming with the peptide, yet neither method allowed accurate measurement of dissociation rates for both complexes because of a lack of resolution. High‐speed CE, with a 7 s separation time, enabled separation of both complexes and allowed determination of dissociation rate of both complexes independently. The two complexes had half‐lives of 22.0±2.7 and 58.8±6.1 s, respectively. Concentration studies revealed that the GST‐Fyn‐SH3‐SH2 protein formed a dimer so that complexes had binding ratios of 2:1 (protein‐to‐peptide ratio) and 2:2. Our results demonstrate that although all methods are suitable for 1:1 binding systems, high‐speed CE is unique in allowing multiple complexes to be resolved simultaneously. This property allows determination of binding kinetics of complicated systems and makes the technique useful for discovering novel affinity interactions.
Bounds on outputs of the exact weak solution of the three‐dimensional Stokes problem
Authors: Cheng Zhong , Ghomeshi Shahin , Paraschivoiu Marius 
Published: January 2009

AbstractA method for obtaining rigorous upper and lower bounds on an output of the exact weak solution of the three‐dimensional Stokes problem is described. Recently bounds for the exact outputs of interest have been obtained for both the Poisson equation and the advection‐diffusion‐reaction equation. In this work, we extend this approach to the Stokes problem where a novel formulation of the method also leads to a simpler flux calculation based on the directly equilibrated flux method. To illustrate this technique, bounds on the flowrate are calculated for an incompressible creeping flow driven by a pressure gradient in an endless square channel with an array of rectangular obstacles in the center. Copyright © 2009 John Wiley & Sons, Ltd.
On comparison of exact and series solutions for thin film flow of a third‐grade fluid
Authors: Abbasbandy S. , Hayat T. , Mahomed F. M. , Ellahi R. 
Published: January 2009

AbstractThe present investigation derives the exact and series solutions for steady thin film flow of a third‐grade fluid. The series solution is constructed by a homotopy analysis method. The obtained solutions are compared and an excellent agreement between these is achieved. Copyright © 2009 John Wiley & Sons, Ltd.
Interactions between endothelial cells and electrospun methacrylic terpolymer fibers for engineered vascular replacements
Authors: Veleva A. N. , Heath D. E. , Johnson J. K. , Nam J. , Patterson C. , Lannutti J. J. , Cooper S. L. 
Published: January 2009

AbstractA compliant terpolymer made of hexylmethacrylate (HMA), methylmethacrylate (MMA), and methacrylic acid (MAA) intended for use in small diameter vascular graft applications has been developed. The mechanical properties and in vitro biostability of this terpolymer have been previously characterized. The goal of this investigation was to examine the interactions between endothelial cells and the new terpolymer and to evaluate endothelial cell function. Electrospinning was used to produce both oriented and random terpolymer fiber scaffolds. Smooth solution cast films and tissue culture polystyrene were used as negative and positive controls, respectively. Human blood outgrowth endothelial cells and human umbilical vein endothelial cells were incubated with the test and control samples and characterized with respect to initial cell attachment, proliferation, viability, and maintenance of the endothelial cell phenotype. It was found that the terpolymer is cytocompatible allowing endothelial cell growth, with random fibers being more effective in promoting enhanced cellular activities than oriented fibers. In addition, endothelial cells cultured on these substrates appeared to maintain their phenotype. The results from this study demonstrate that electrospun HMA:MMA:MAA terpolymer has the potential to be used successfully in fabricating small diameter blood vessel replacements. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Hydrogel‐mediated DNA delivery confers estrogenic response in nonresponsive osteoblast cells
Authors: Dadsetan M. , Szatkowski J. P. , Shogren K. L. , Yaszemski M. J. , Maran A. 
Published: January 2009

AbstractOligo(polyethylene glycol) fumarate (OPF) hydrogel has been employed in musculoskeletal tissue engineering for photoencapsulation of chondrocytes and as a matrix for marrow stromal cells differentiation. In this study, we have studied the application of OPF hydrogel for coencapsulation of DNA and bone cells and examined whether coencapsulation can enhance gene transfer by maintaining the DNA within the cellular microenvironment. Our results showed that plasmid DNA encoding green fluorescence protein (GFP), coencapsulated with bone tumor cells, was capable of transfecting the cells, and the transfected tumor cells continuously expressed GFP protein over the time course of study (21 days). Furthermore, we have examined the coencapsulation of estrogen receptor (ER) encoding plasmid DNA and human fetal osteoblast cells (hFOB) that lack endogenous ER. Our results show that the transfected cells responded to estrogen as alkaline phosphatase (ALP), and estrogen response element (ERE)‐directed luciferase enzyme activities increased with estrogen treatment. Taken together, these studies show that OPF hydrogel could be further explored for targeted gene delivery in bone and other tissues encapsulated within the hydrogels. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Cell micropatterning on an albumin‐based substrate using an inkjet printing technique
Authors: Yamazoe Hironori , Tanabe Toshizumi 
Published: January 2009

AbstractPositioning of cells in a desired pattern on a substrate is an important technique for cell‐based technologies, including the fundamental investigation of cell functions, tissue‐engineering applications, and the fabrication of cell‐based biosensors and cell arrays. Recently, the inkjet printing technique was recognized as a promising approach to the creation of cellular patterns on substrates, and it has been achieved by the printing of living cells or cell adhesive proteins. In this article, we created complex cellular patterns by using an albumin‐based substrate and inkjet printing technique. Albumin was cross‐linked using ethylene glycol diglycidyl ether. Subsequent casting of the cross‐linked albumin solution onto glass plates prevented cells from adhering to their surfaces. Through screening various chemical reagents, we found that these cross‐linked albumin surfaces dramatically changed into cell adhesive surfaces after immersion in cationic polymer solutions. Based on this finding, cell adhesive regions were prepared with a desired pattern by printing the polyethyleneimine (PEI) solution onto a cross‐linked albumin substrate using a modified commercial inkjet printer. Various cellular patterns including figures, letters, and gradients could be fabricated by seeding mouse L929 fibroblast cells or mouse Neuro‐2a neuroblastoma cells onto the printed PEI‐patterned substrate. Compared with the printing of fragile living cells or proteins, printing of stable PEI circumvents clogging of printer head nozzles and enables reproducible printing. Therefore, the present method will allow the creation of complex cell patterns. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Protein‐resistant polyurethane via surface‐initiated atom transfer radical polymerization of oligo(ethylene glycol) methacrylate
Authors: Jin Zhilin , Feng Wei , Zhu Shiping , Sheardown Heather , Brash John L. 
Published: January 2009

AbstractProtein‐resistant polyurethane (PU) surfaces were prepared by surface‐initiated simultaneous normal and reverse atom transfer radical polymerization (s‐ATRP) of poly(oligo(ethylene glycol) methacrylate) (poly (OEGMA)). Oxygen plasma treatment was employed for initial activation of the PU surface. The grafted polymer chain length was adjusted by varying the molar ratio of monomer to sacrificial initiator in solution from 5:1 to 200:1. The modified PU surfaces were characterized by water contact angle, X‐ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). Protein adsorption experiments from tris‐buffered saline (TBS) and plasma were carried out to evaluate the protein‐resistance of the surfaces. Adsorption from single and binary protein solutions as well as from plasma was significantly reduced after modification. Adsorption decreased with increasing poly(OEGMA) chain length. Fibrinogen (Fg) adsorption on the 200:1 monomer/initiator surface was in the range of 3–33 ng/cm2 representing 96–99% reduction compared with the unmodified PU. Fg adsorption from 0.01–10% plasma was as low as 1–5 ng/cm2. Moreover, binary protein adsorption experiments using Fg and lysozyme (Lys) showed that protein size is a factor in the protein resistance of these surfaces. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Effective gait patterns for offloading the medial compartment of the knee
Authors: Fregly Benjamin J. , D'Lima Darryl D. , Colwell Clifford W. 
Published: January 2009

AbstractGait modification offers a noninvasive option for offloading the medial compartment of the knee in patients with knee osteoarthritis. While gait modifications have been proposed based on their ability to reduce the external knee adduction moment, no gait pattern has been proven to reduce medial compartment contact force directly. This study used in vivo contact force data collected from a single subject with a force‐measuring knee replacement to evaluate the effectiveness of two gait patterns at achieving this goal. The first was a “medial thrust” gait pattern that involved medializing the knee during stance phase, while the second was a “walking pole” gait pattern that involved using bilateral walking poles commonly used for hiking. Compared to the subject's normal gait pattern, medial thrust gait produced a 16% reduction and walking pole gait a 27% reduction in medial contact force over stance phase, both of which were statistically significant based on a two‐tailed Mann–Whitney U‐test. While medial thrust gait produced little change in lateral and total contact force over the stance phase, walking pole gait produced significant 11% and 21% reductions, respectively. Medial thrust gait may allow patients with knee osteoarthritis to reduce medial contact force using a normal‐looking walking motion requiring no external equipment, while walking pole gait may allow patients with knee osteoarthritis or a knee replacement to reduce medial, lateral, and total contact force in situations where the use of walking poles is possible. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 1016–1021, 2009
Effect of feeding intact brown seaweed Ascophyllum nodosum on some digestive parameters and on iodine content in edible tissues in pigs
Authors: Dierick Noël , Ovyn Anneke , De Smet Stefaan 
Published: January 2009

AbstractBACKGROUND: Limited research suggests that brown seaweed (extracts) may be used in pig nutrition for improving gut health and performances and for iodine enrichment of tissues. One in vitro and two in vivo experiments with dried iodine‐rich intact marine seaweed (Ascophyllum nodosum) have been conducted with weaned piglets to further unravel the mechanisms.RESULTS: In vitro investigations revealed a statistically significant depressive effect of seaweed on pig gut flora, especially on Escherichia coli. In vivo, seaweed (10 g kg−1) had a reducing effect on the E. coli load in the stomach (P = 0.07) and small intestine (P < 0.05), while the lactobacilli/E. coli ratio was enhanced (P < 0.05) in the small intestine, indicating a beneficial shift in the microbial population. Statistically significant increases (P < 0.001) in iodine content were noted for several tissues in piglets on seaweed (20 g kg−1, corresponding to 10 mg iodine kg−1 feed) compared with the control diet (1 mg iodine kg−1 feed).CONCLUSION: Intact A. nodosum brown seaweed may be introduced in pig nutrition as a feed material with a double strategy: improvement of pig gut health and performances and iodine enrichment of porcine tissues. This feeding strategy may alleviate, but not solve, the actual iodine deficiency in Belgium. Copyright © 2009 Society of Chemical Industry
Phage Tailspike Proteins with β‐Solenoid Fold as Thermostable Carbohydrate Binding Materials
Authors: Barbirz Stefanie , Becker Marion , Freiberg Alexander , Seckler Robert 
Published: January 2009

AbstractWe have investigated the stability of three tailspike proteins (TSPs) from bacteriophages Sf6, P22, and HK620. Tailspikes are rod‐like homotrimers with comparable β‐solenoid folds and similarly high kinetic stability in spite of different amino acid sequences. As tailspikes bind polysaccharides to recognize the bacterial host cell, their stability is required for maintenance of bacteriophage infectivity under harsh extracellular conditions. They resist denaturation by SDS at ambient temperature and their unfolding is slow even in 6 M guanidinium hydrochloride (GdmHCl). This makes them interesting candidates for very stable carbohydrate binding protein materials.magnified image
Pressure‐Sensitive Chromogenic Polyesters
Authors: Kunzelman Jill , Gupta Mohit , Crenshaw Brent R. , Schiraldi David A. , Weder Christoph 
Published: January 2009

AbstractCyano‐OPV moieties were covalently incorporated into a PETG backbone to create ductile amorphous polymers which change their PL and absorption color as a function of deformation. The cyano‐OPV concentration was systematically varied, and the composition was related to the material's optical response. This approach afforded PETG/dye copolymers which upon annealing display characteristics of aggregated dye molecules. The materials exhibit a significant color change upon compression, consistent with disassembly of the dye aggregates. This mechanochromic response is irreversible and can be detected by the unassisted eye.magnified image
Correlated downregulation of estrogen receptor beta and the circadian clock gene Per1 in human colorectal cancer
Authors: Mostafaie Nazanin , Kállay Enikö , Sauerzapf Elisabeth , Bonner Elisabeth , Kriwanek Stefan , Cross Heide S. , Huber Klaus Roland , Krugluger Walter 
Published: January 2009

AbstractThere is a growing body of evidence that disturbed circadian clock gene expression is associated with tumor development and tumor progression. Based on our initial experiments demonstrating decreased period 1 (Per1) expression in colon cancer, we evaluated clock gene and estrogen receptor (ER) alpha/beta expression in colon cancer cells of primary colorectal tumors and adjacent normal colon mucosa (NM) by real‐time RT‐PCR. Analysis of gene expression in G2 and G3 colorectal tumors revealed a decrease of Per1 mRNA compared with paired NM (G2: 0.52‐fold; P = n.s. and G3: 0.48‐fold; P = 0.03). A significant gender specific difference of Per1 expression was observed in G2 tumors as compared with NM (female: 0.38‐fold; P = 0.004 vs. male: 0.73‐fold; P = n.s.). Expression of CLOCK was significantly elevated in G2 tumors of male patients (1.63‐fold, P = 0.01). The expression of ER‐beta was significantly decreased in G2 and G3 tumors (G2: 0.32‐fold; P = 0.003 and 0.27; P = 0.001). No significant gender specific differences of ER‐beta reduction in tumors were observed. A significant correlation between the decrease of Per1 and ER‐beta in colorectal tumors (r = 0.61; P < 0.001) was found. No changes in gene expression were detected for ER‐alpha and Per2. Our data demonstrate a correlated decrease of Per1 and ER‐beta in colorectal tumors, mediated probably by epigenetic mechanisms. The observed gender differences in the expression of CLOCK and Per1 in G2 tumors might suggest a gender‐specific, distinctive role of the cellular clock in colorectal tumorigenesis. © 2009 Wiley‐Liss, Inc.
On the Cascade Polymerization Process Consisting of Metallocene Polymerization and ATRP to Prepare i‐PP‐Based Polar Block Copolymers
Authors: Yi Qingfeng , Fan Guoqiang , Wen Xiaojing , Dong Jin‐Yong , Han Charles C. 
Published: January 2009

AbstractIsotactic poly(propylene)s bearing a terminal hydroxyl group (i‐PP‐t‐OH) with high degrees of end‐group functionality were synthesized by a metallocene/methylaluminoxane (MAO)‐mediated propylene polymerization process via a preferential aluminium chain transfer reaction assisted by a hydroalumination process at the end of polymerization. Despite the highly isotactic configuration and relatively high molecular weight ($\overline M _{\rm n}$ as high as 60 800 g · mol−1) of the polymers, the hydroxyl group located at the i‐PP chain terminal was efficiently transformed to an atom transfer radical polymerization (ATRP) initiator moiety of i‐PP‐t‐Br via a reaction with 2‐bromopropionyl chloride at 60 °C in toluene. Well‐defined i‐PP‐b‐PMMA and i‐PP‐b‐PS block copolymers of controllable structure and compositions were then prepared with efficient ATRPs of MMA and St using i‐PP‐t‐Br.magnified image
An exploratory analysis of the effect of the “buy it now” option on sellers' revenues in silent auctions
Authors: Page Karen 
Published: January 2009

Abstract

This paper tests three competing hypotheses regarding the effect of a “buy it now” option in silent auctions: that the option has a positive, negative, or neutral effect on the seller's revenues. These hypotheses were tested on 4 years of data from a university art museum's silent auctions. The results indicate that the “buy it now” option has a significant negative effect on the seller's revenues. Possible explanation, implications, and limitations are discussed.
Copyright © 2009 John Wiley & Sons, Ltd.
Analysis of ototoxicity in young children receiving carboplatin in the context of conservative management of unilateral or bilateral retinoblastoma
Authors: Jehanne Mathilde , Lumbroso‐Le Rouic Livia , Savignoni Alexia , Aerts Isabelle , Mercier Ghislaine , Bours Danielle , Desjardins Laurence , Doz François 
Published: January 2009

AbstractBackgroundCarboplatin plays an important role in the conservative management of retinoblastoma, but is associated with risk of ototoxicity in these young children whose sensory prognosis may be also compromised by their loss of vision. This retrospective study analyzed the impact of carboplatin on hearing in the context of conservative management of children with retinoblastoma.MethodsData for 175 children treated at the Institut Curie between 1994 and 2002 were analyzed.ResultsMedian age at diagnosis was 8 months (0–60). Carboplatin was administered on 3 days (200 mg/m2/day) or 5 days (160 mg/m2/day) with etoposide and with diode‐laser therapy at the dose of 560 mg/m2 (chemothermotherapy). Median cumulative dose of carboplatin was 2,880 mg/m2 (560–6,160). Ototoxicity was investigated by pure‐tone audiometry and scored by Brock's grading scale before and after treatment. The median follow‐up of hearing assessment was 5 years (1.8–11). Ototoxicity was detected in 8 children: 3 grade 1, 1 grade 2, and 2 grade 4. The two patients with grade 4 hearing‐loss required a hearing aid. Two children developed bilateral high frequency hearing‐loss, considered to be secondary to carboplatin but with less than Brock grade 1. Ototoxicity was observed for a median cumulative dose of carboplatin of 3,120 mg/m2 (1,200–5,830). Only one child developed ototoxicity during treatment. All other cases were discovered after the last dose of carboplatin with a median interval of 3.7 years (0–7.6). No other risk factor for ototoxicity was able to account for these lesions.ConclusionChildren receiving carboplatin require long‐term audiometric follow‐up. Pediatr Blood Cancer 2009;52:637–643. © 2009 Wiley‐Liss, Inc.
Concurrent Langerhans cell histiocytosis and nephroblastoma
Authors: Narui Ryoko , Yagasaki Hiroshi , Takahashi Yoshiyuki , Hama Asahito , Nishio Nobuhiro , Muramatsu Hideki , Shimoyama Yoshie , Kojima Seiji 
Published: January 2009

AbstractBoth Langerhans cell histiocytosis (LCH) and nephroblastoma are rare in children. We report herein the first case of a patient with both diseases concurrently. A 2‐year‐old female presented with bone pain and swelling of the right humerus. As a result of the local incision biopsy, she was diagnosed as LCH. A nephroblastoma of the left kidney was discovered during her staging work‐up. After complete resection of the nephroblastoma, she received standard chemoradiotherapy for nephroblastoma. She is alive without relapse 14 months after initial presentation. Pediatr Blood Cancer 2009;52:662–664. © 2009 Wiley‐Liss, Inc.
Adverse drug reaction monitoring: comparing doctor and patient reporting for new drugs
Authors: Jarernsiripornkul N. , Kakaew W. , Loalukkana W. , Krska J. 
Published: January 2009

AbstractPurposeTo compare the frequency of adverse drug reactions (ADRs) recorded by doctors with perceived ADRs reported by patients during hospitalization and post‐discharge and to compare both to formal reporting in Thailand.SettingTertiary teaching hospital, North‐East Thailand.MethodsOver a 6‐month period doctors were requested to record ADRs to 13 recently marketed index drugs, occurring during hospitalization and after discharge, using specific forms. Patient perceived ADRs were detected by daily pharmacist interviews during hospitalization and a symptom checklist questionnaire after discharge.ResultsIn total, 203 in‐patients were prescribed an index drug and interviewed. Doctors recorded 13 ADRs in these patients, resulting in seven drugs being stopped. In‐patients reported 15 further symptoms, but 12 were assessed as unlikely to be an ADR. Of 160 patients who continued the index drug after discharge, 152 accepted questionnaires and 88 (58%) were returned. Patients identified 249 symptoms, of which the pharmacist assessed 190 (76%) as probably or possibly ADRs, using a previously developed method. Only 26 symptoms were noted in out‐patient department (OPD) records, although most patients claimed to have informed doctors about them. Doctors recorded only five symptoms on ADR forms, all associated with drug discontinuation. During the study period, four reports were received by the Thai FDA on these 13 drugs, none from this hospital.ConclusionsForms encouraging doctors to identify ADRs may be an effective method of increasing recording, particularly in the in‐patient setting. A combination of methods including patient reporting may enable pharmacists to increase formal ADR reporting. Copyright © 2009 John Wiley & Sons, Ltd.
Preparation of ZnO nanoparticles for bio‐imaging applications
Authors: Senthilkumar K. , Senthilkumar O. , Yamauchi Kazuki , Sato Moriyuki , Morito Shigekazu , Ohba Takuya , Nakamura Morihiko , Fujita Yasuhisa 
Published: January 2009

AbstractZnO nanoparticles were prepared by using a gas evaporation method. As ZnO is a luminescent and less toxic material, it can be useful in imaging applications. The nanoparticles prepared in our laboratory showed intense exciton emission in the UV‐blue region. They were processed and surface treated to be compatible for bio‐conjugation. We were able to prepare good dispersions and water‐soluble ZnO nanoparticles from our experiments. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)