bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seven percent of primary anterior crucial ligament reconstruction patients have arthroscopic resection of cyclops lesions within 2 years: A cohort study of 2556 patients
Authors: Miller Lene Lindberg , Lind Martin , Mechlenburg Inger , Nielsen Torsten Grønbech 
Published: April 2024

AbstractPurposeAfter anterior cruciate ligament reconstruction (ACL‐R), a localised scar tissue called cyclops lesion may develop anterior to the graft causing knee extension deficits, pain, oedema, clicking and reduced knee function. This study determined the incidence of arthroscopic resection of a cyclops lesion within 2 years after ACL‐R and investigated the associations of patient characteristics and surgical techniques with the need for arthroscopic resection of a cyclops lesion.MethodsThis study included patients who underwent primary ACL‐R with adult surgical technique from 2005 to 2019 at Aarhus University Hospital, Denmark. The cohort was identified in a national registry. To identify patients who had resected a cyclops lesion within the first 2 years after ACL‐R, patients' surgical records were reviewed.ResultsIn 2005–2019, 2556 patients underwent primary ACL‐R; 176 developed cyclops lesions that were resected within 2 years, equivalent to an incidence of 6.9% (95% confidence interval [CI]: 5.9–7.9). When stratified by the femoral drilling technique used, this incidence was 8.9% (95% CI: 7.7–10.3) with the anteromedial technique and 1.9% (95% CI: 1.0–3.1) with the transtibial technique. The incidence was 8.5% (95% CI: 6.8–10.3) in women and 5.7% (95% CI: 4.6–7.1) in men. Age, graft choice and the presence of cartilage or meniscal lesions did not affect the incidence.ConclusionThe overall incidence of a cyclops lesion removal within 2 years post‐ACL‐R was 6.9%. This was five times higher with the anteromedial femoral drilling technique than with the transtibial technique. Women had a 47% higher incidence of cyclops lesion removal than men. This is relevant for the surgeon when planning an ACL‐R.Level of EvidenceLevel II
Low implantation volume, comorbidities, male sex and implantation of constrained TKA identified as risk factors for septic revision in knee arthroplasty: A register‐based study from the German Arthroplasty Registry
Authors: Szymski Dominik , Walter Nike , Straub Josina , Wu Yinan , Melsheimer Oliver , Grimberg Alexander , Alt Volker , Steinbrueck Arnd , Rupp Markus 
Published: April 2024

AbstractPurposePeriprosthetic joint infection (PJI) is a major cause of revision surgery after total knee arthroplasty (TKA) and unicondylar knee arthroplasty (UKA). Patient‐ and hospital‐related risk factors need to be assessed to prevent PJI. This study identifies influential factors and differences in infection rates between different implant types.MethodsData were obtained from the German Arthroplasty Registry. Septic revisions were calculated using Kaplan–Meier estimates with septic revision surgery as the primary endpoint. Patients with constrained and unconstrained TKA or UKA were analysed using Holm's multiple log‐rank test and Cox's proportional hazards ratio. The 300,998 cases of knee arthroplasty analysed included 254,144 (84.4%) unconstrained TKA, 9993 (3.3%) constrained TKA and 36,861 (12.3%) UKA with a maximum follow‐up of 7 years.ResultsAt 1 year, the PJI rate was 0.5% for UKA and 2.8% for TKA, whereas at 7 years, the PJI rate was 4.5% for UKA and 0.9% for TKA (p < 0.0001). The PJI rate significantly increased for constrained TKA compared to unconstrained TKA (p < 0.0001). The PJI rate was 2.0% for constrained TKA and 0.8% for unconstrained TKA at 1 year and 3.1% and 1.4% at 7 years. Implantation of a constrained TKA (hazard ratio [HR] = 2.55), male sex (HR = 1.84), increased Elixhauser score (HR = 1.18–1.56) and implant volume of less than 25 UKA per year (HR = 2.15) were identified as risk factors for revision surgery; an Elixhauser score of 0 (HR = 0.80) was found to be a preventive factor.ConclusionsReduced implant volume and constrained knee arthroplasty are associated with a higher risk of PJI. Comorbidities (elevated Elixhauser score), male sex and low UKA implant volume have been identified as risk factors for PJI. Patients who meet these criteria require specific measures to prevent infection. Further research is required on the potential impact of prevention and risk factor modification.Level of EvidenceLevel III.
Numerical Analysis of the Blade Coating Process Using Non‐Newtonian Nanofluid with Magnetohydrodynamic (MHD) and Slip Effects
Authors: Javed Muhammad Asif , Ghaffari Abuzar , Khan Sami Ullah , Elattar Ehab 
Published: April 2024

AbstractThe coating process is widely used in various industries to enhance the production quality and efficiency. This study gives a comprehensive analysis of non‐isothermal blade coating of non‐Newtonian nanofluid incorporating magnetic, thermophoresis, and Brownian effects. The mathematical equations derived from mass, momentum, and energy conservation laws are initially streamlined by means of lubrication approximation theory (LAT). Subsequently, these dimensionless equations are solved in dimensionless form numerically using fourth order Runge–Kutta and Newton–Raphson methods. This study includes the effects of the slip parameter, magnetohydrodynamic (MHD) and other material parameters on the coating thickness (), blade load, velocity, temperature, concentration, and pressure profiles through graphs and tables. The velocity of molten polymer increases near the substrate while it decreases near the blade surface as the slip parameter increases. The temperature distribution increases as the Brinkman number rises, with the maximum temperature occurring in the nip region of the flow. The coating thickness and load‐carrying force for both plane and exponential coater increase with higher values of the magnetohydrodynamic (MHD) parameter.
Benzo[a]pyrene promotes an epithelial‐to‐mesenchymal transition process in MCF10A cells and mammary tumor growth and brain metastasis in female mice
Authors: Castillo‐Sanchez Rocio , Garcia‐Hernandez Alejandra , Torres‐Alamilla Pablo , Cortes‐Reynosa Pedro , Candanedo‐Gonzales Fernando , Salazar Eduardo Perez 
Published: April 2024

AbstractBreast cancer is the most frequent neoplasia in developed countries and the leading cause of death in women worldwide. Epithelial‐to‐mesenchymal transition (EMT) is a cellular process through which epithelial cells decrease or lose their epithelial characteristics and gain mesenchymal properties. EMT mediates tumor progression, because tumor cells acquire the capacity to execute the multiple steps of invasion and metastasis. Benzo[a]pyrene (B[a]P) is an environmental organic pollutant generated during the burning of fossil fuels, wood, and other organic materials. B[a]P exposition increases the incidence of breast cancer, and induces migration and/or invasion in MDA‐MB‐231 and MCF‐7 breast cancer cells. However, the role of B[a]P in the induction of an EMT process and metastasis of mammary carcinoma cells has not been studied in detail. In this study, we demonstrate that B[a]P induces an EMT process in MCF10A mammary non‐tumorigenic epithelial cells. In addition, B[a]P promotes the formation of larger tumors in Balb/cJ mice inoculated with 4T1 cells than in untreated mice and treated with dimethyl sulfoxide (DMSO). B[a]P also increases the number of mice with metastasis to brain and the total number of brain metastatic nodules in Balb/cJ mice inoculated with 4T1 cells compared with untreated mice and treated with DMSO. In conclusion, B[a]P induces an EMT process in MCF10A cells and the growth of mammary tumors and metastasis to brain in Balb/cJ mice inoculated with 4T1 cells.
Four and a half LIM domains 2 (FHL2) attenuates tumorigenesis of gastrointestinal stromal tumors (GISTs) by negatively regulating KIT signaling
Authors: Zhang Shaoting , Zhang Liangying , Zhang Dan , Guo Yue , Gao Yisha , Jiang Zongying , Li Shujing , Liu Anbu , Cao Xu , Tian Jinhai , Zhao Sien , Yu Yuanyuan , Yang Wei , Bai Ru , Huang Ling , Yan Hongli , Zhao Hui , Sun Jianmin 
Published: April 2024

AbstractGastrointestinal stromal tumors (GISTs) are predominately induced by KIT mutants. In this study, we found that four and a half LIM domains 2 (FHL2) was highly expressed in GISTs and KIT signaling dramatically increased FHL2 transcription while FHL2 inhibited KIT transcription. In addition, our results showed that FHL2 associated with KIT and increased the ubiquitination of both wild‐type KIT and primary KIT mutants in GISTs, leading to decreased expression and activation of KIT although primary KIT mutants were less inhibited by FHL2 than wild‐type KIT. In the animal experiments, loss of FHL2 expression in mice carrying germline KIT/V558A mutation which can develop GISTs resulted in increased tumor growth, but increased sensitivity of GISTs to imatinib treatment which is used as the first‐line targeted therapy of GISTs, suggesting that FHL2 plays a role in the response of GISTs to KIT inhibitor. Unlike wild‐type KIT and primary KIT mutants, we further found that FHL2 didn't alter the expression and activation of drug‐resistant secondary KIT mutants. Taken together, our results indicated that FHL2 acts as the negative feedback of KIT signaling in GISTs while primary KIT mutants are less sensitive and secondary KIT mutants are resistant to the inhibition of FHL2.
High gain D‐band hybrid integrated on‐chip antenna based on low‐cost printed circuit board packaging technology
Authors: Wang Yanjun , Yi Xiang , Che Wenquan , Xue Quan 
Published: April 2024

AbstractA high gain D‐band hybrid integrated on‐chip antenna (OCA) based on a low‐cost printed circuit board (PCB) packaging technology is analyzed and designed in a standard 40‐nm CMOS process in this paper. The proposed OCA is a hybrid structure of a slotted patch OCA with off‐chip PCB ground and off‐chip dielectric resonator (DR). The proposed OCA exhibits a simulated gain of 3.5 dBi and 53% radiation efficiency at 150 GHz. The simulated impedance bandwidth is 20 GHz. The radiation performance and impedance bandwidth of the OCA are observably improved with the proposed techniques. The proposed OCA occupies 0.52 × 0.56 mm2, and addresses the highly desirable yet challenging overall design of future low‐cost integrated millimeter‐wave (mm‐Wave) and terahertz (THz) systems, which hit the mark on cost and compatibility.
Age estimation and validation in otoliths, spines, and fin rays from four central Texas fishes
Authors: Pfennig Meredith B. , Crane Derek P. , Smith Nate G. , Buckmeier Dave L. 
Published: April 2024

Abstract

Objective
Age estimation methods have not been validated for many species. Thus, we focused this age estimation study on four species from central Texas: the Guadalupe Bass Micropterus treculii, Channel Catfish Ictalurus punctatus, Gray Redhorse Moxostoma congestum, and Redbreast Sunfish Lepomis auritus. Our objectives for each species were to (1) investigate whether increments form annually in otoliths, fin rays, or spines; (2) determine the seasonal timing of zone deposition in each calcified structure; and (3) compare age estimates between otoliths and fin rays or spines.

Methods
Fish were sampled from central Texas waters and injected with oxytetracycline (OTC) quarterly in 2021. In July 2022, otoliths and fin rays or spines were collected to determine whether the increments formed annually and to estimate age.

Result
On average, two readers identified the correct number of annuli after the OTC mark in at least 95% of Gray Redhorse, Guadalupe Bass, and Redbreast Sunfish otoliths but in only 69% of Channel Catfish otoliths. However, the more experienced reader identified the correct number of annuli in at least 95% of otoliths from age-2–4 Channel Catfish. Compared to otoliths, the correct number of annuli after the OTC mark was identified in a smaller percentage of fin rays or spines (47–62%). The probability of identifying the correct number of annuli after the OTC mark differed between the two readers for all spines and fin rays. Age difference plots revealed that age estimates based on fin rays or spines were either overestimated or underestimated compared to otolith-based ages for all species.

Conclusion
We consider annual increment formation in otoliths to be validated for all age-classes of Gray Redhorse, Guadalupe Bass, and Redbreast Sunfish examined and for ages 2–4 of Channel Catfish. Differences in correctness between readers were most likely due to differences in age estimation experience. Given the level of error observed in our study, caution is advised if using age estimates from unvalidated structures and species.
Work experience of breastfeeding nurses returning to work after maternity leave in Liaoning Province of China: A qualitative study
Authors: Wan Xin‐li , Yang Jia‐yi , Pan Ying‐li 
Published: April 2024

AbstractAimWith the implementation of China's three‐child policy in 2021, the nurse population faces an increase in the number of breastfeeding nurses returning to work after maternity leave. This study aims to describe the work experience of breastfeeding nurses returning to work after maternity leave.DesignA qualitative descriptive design.MethodsThe data were collected through semi‐structured interviews with eight nurses and analysed through Braun and Clarke's thematic analysis.ResultsThree themes and nine sub‐themes emerged from the analysis of the interviews: changes in nurses (emotional changes, physical changes and changes in work); needs for an improving work environment (needs for a supportive workplace and nurse shortage); support for breastfeeding nurses (support from coworkers, support from the manager, support from the organisation and own need for work).Public ContributionThis study highlighted that breastfeeding nurses need an adjustment period when they return to work after maternity leave. Successful breastfeeding requires support from coworkers, managers and the organisation. In addition, workplace support for breastfeeding and management for nurses needs to be improved.
RAF1 gene fusions are recurrent driver events in infantile fibrosarcoma‐like mesenchymal tumors
Authors: Motta Marialetizia , Barresi Sabina , Pizzi Simone , Bifano Delfina , Lopez Marti Jennifer , Garrido‐Pontnou Marta , Flex Elisabetta , Bruselles Alessandro , Giovannoni Isabella , Rotundo Giovannina , Fragale Alessandra , Tirelli Valentina , Vallese Silvia , Ciolfi Andrea , Bisogno Gianni , Alaggio Rita , Tartaglia Marco 
Published: April 2024

AbstractInfantile fibrosarcomas (IFS) and congenital mesoblastic nephroma (CMN) are rare myofibroblastic tumors of infancy and early childhood commonly harboring the ETV6::NTRK3 gene fusion. IFS/CMN are considered as tumors with an ‘intermediate prognosis’ as they are locally aggressive, but rarely metastasize, and generally have a favorable outcome. A fraction of IFS/CMN‐related neoplasms are negative for the ETV6::NTRK3 gene rearrangement and are characterized by other chimeric proteins promoting MAPK signaling upregulation. In a large proportion of these tumors, which are classified as IFS‐like mesenchymal neoplasms, the contributing molecular events remain to be identified. Here, we report three distinct rearrangements involving RAF1 among eight ETV6::NTRK3 gene fusion‐negative tumors with an original histological diagnosis of IFS/CMN. The three fusion proteins retain the entire catalytic domain of the kinase. Two chimeric products, GOLGA4::RAF1 and LRRFIP2::RAF1, had previously been reported as driver events in different cancers, whereas the third, CLIP1::RAF1, represents a novel fusion protein. We demonstrate that CLIP1::RAF1 acts as a bona fide oncoprotein promoting cell proliferation and migration through constitutive upregulation of MAPK signaling. We show that the CLIP1::RAF1 hyperactive behavior does not require RAS activation and is mediated by constitutive 14‐3‐3 protein‐independent dimerization of the chimeric protein. As previously reported for the ETV6::NTRK3 fusion protein, CLIP1::RAF1 similarly upregulates PI3K‐AKT signaling. Our findings document that RAF1 gene rearrangements represent a recurrent event in ETV6::NTRK3‐negative IFS/CMN and provide a rationale for the use of inhibitors directed to suppress MAPK and PI3K‐AKT signaling in these cancers. © 2024 The Pathological Society of Great Britain and Ireland.
ΔNp73 and its effector targets promote colorectal peritoneal carcinosis and predict survival
Authors: Pastor‐Morate Daniel , Amigo‐Morán Lidia , Garranzo‐Asensio María , Rejas‐González Raquel , Carnicero Patricia , Rodríguez Nuria , Pérez‐Robledo Juan Pedro , Barderas Rodrigo , Prieto‐Nieto Isabel , Domínguez Gemma 
Published: April 2024

AbstractPeritoneal metastasis of colorectal origin appears in ~10–15% of patients at the time of diagnosis and in 30–40% of cases with disease progression. Locoregional spread through the peritoneum is considered stage IVc and is associated with a poor prognosis. The development of a regional therapeutic strategy based on cytoreductive surgery, and hyperthermic intra‐abdominal chemotherapy has significantly altered the course of the disease. Although recent evidence supports the benefits of cytoreductive surgery, the benefits of hyperthermic intra‐abdominal chemotherapy are, however, still a matter of debate. Understanding the molecular alterations underlying the disease is crucial for developing new therapeutic strategies. Here, we evaluated the involvement in peritoneal dissemination of the oncogenic isoform of TP73, ΔNp73, and its effector targets in in vitro and mouse models, and in 30 patients diagnosed with colorectal peritoneal metastasis. In an orthotopic mouse model, we observed that tumor cells overexpressing ΔNp73 present a higher avidity for the peritoneum and that extracellular vesicles secreted by ΔNp73‐upregulating tumor cells enhance their dissemination. In addition, we identified that tumor cells overexpressing ΔNp73 present with dysregulation of genes associated with an epithelial/mesothelial‐to‐mesenchymal transition (MMT) and that mesothelial cells exposed to the conditioned medium of tumor cells with upregulated ΔNp73 present a mesenchymal phenotype. Lastly, ΔNp73 and its effector target RNAs were dysregulated in our patient series, there were positive correlations between ΔNp73 and its effector targets, and MSN and ITGB4 (ΔNp73 effectors) predicted patient survival. In conclusion, ΔNp73 and its effector targets are involved in the peritoneal dissemination of colorectal cancer and predict patient survival. The promotion of the EMT/MMT and modulation of the adhesion capacity in colorectal cancer cells might be the mechanisms triggered by ΔNp73. Remarkably, ΔNp73 protein is a druggable protein and should be the focus of future studies. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.
Event‐free survival in relapsed and refractory rhabdomyosarcoma treated on cooperative group phase II trials: A report from the Children's Oncology Group
Authors: Metts Jonathan , Xue Wei , Gao Zhengya , Oberoi Sapna , Weiss Aaron R. , Venkatramani Rajkumar , Harrison Douglas J. 
Published: April 2024

AbstractBackgroundNovel therapies are needed for relapsed and refractory rhabdomyosarcoma (RRMS). Phase II clinical trials in RRMS have typically utilized radiologic response as the primary activity endpoint, an approach that poses several limitations in RRMS. In this analysis, we aimed to estimate an event‐free survival (EFS) endpoint for RRMS that could be used as a benchmark for future studies.ProcedureWe performed a retrospective study of patients with RRMS enrolling on 13 single‐agent phase II Children's Oncology Group and legacy group trials from 1997 to 2016. All included trials used radiographic response as their primary activity endpoint. Six‐month EFS was estimated from time of trial enrollment with 95% confidence intervals. Clinical characteristics, including trial of enrollment, sex, age, race, histology, number of prior chemotherapies, and radiographic response were evaluated for their impact on 6‐month EFS.ResultsWe identified 175 patients across 13 trials. The 6‐month EFS was 16.8% (11.6%–22.8%). No differences were seen in 6‐month EFS based on age, sex, race, or histology. There were nonsignificant trends toward improved 6‐month EFS for patients with less than or equal to two prior lines of therapy versus higher than two, for patients enrolled on trials that achieved their primary radiographic response endpoint versus trials that did not, and for patients who achieved complete or partial response compared to those achieving stable disease.ConclusionsThe prognosis of RRMS enrolled on single‐agent phase II trials is poor. This pooled 6‐month EFS of RRMS on single‐agent trials may be used as a RRMS‐specific benchmark for future single‐agent phase II trials.
Updated core competencies in pharmacoepidemiology to inform contemporary curricula and training for academia, government, and industry
Authors: Osborne Vicki , Goodin Amie , Brown Joshua , Winterstein Almut G. , Bate Andrew , Cohet Catherine , Pont Lisa , Moeny David , Klungel Olaf , Pinheiro Simone , Seeger John , Chan K. Arnold , Edlavitch Stanley , Tilson Hugh , Layton Deborah 
Published: April 2024

AbstractPurposeThe first paper to specify the core content of pharmacoepidemiology as a profession was published by an ISPE (International Society for Pharmacoepidemiology) workgroup in 2012 (Jones JK et al. PDS 2012; 21[7]:677–689). Due to the broader and evolving scope of pharmacoepidemiology, ISPE considers it important to proactively identify, update and expand the list of core competencies to inform curricula of education programs; thus, better positioning pharmacoepidemiologists across academic, government (including regulatory), and industry positions. The aim of this project was to update the list of core competencies in pharmacoepidemiology.MethodsTo ensure applicability of findings to multiple areas, a working group was established consisting of ISPE members with positions in academia, industry, government, and other settings. All competencies outlined by Jones et al. were extracted from the initial manuscript and presented to the working group for review. Expert‐based judgments were collated and used to identify consensus. It was noted that some competencies could contribute to multiple groups and could be directly or indirectly related to a group.ResultsFive core domains were proposed: (1) Epidemiology, (2) Clinical Pharmacology, (3) Regulatory Science, (4) Statistics and data science, and (5) Communication and other professional skills. In total, 55 individual competencies were proposed, of which 25 were new competencies. No competencies from the original work were dropped but aggregation or amendments were made where considered necessary.ConclusionsWhile many core competencies in pharmacoepidemiology have remained the same over the past 10 years, there have also been several updates to reflect new and emerging concepts in the field.
Applying structural equation modeling to examine the role of teacher beliefs and practices in differentiated instruction in physical education: Multiple mediation analyses
Authors: Wen Qingyun , Cai Juan 
Published: April 2024

AbstractDifferentiated instruction remains one of the most important frameworks for the global education community to achieve sustainable educational development goals and inclusive education. It is also one of the most critical challenges for teachers in different subjects. This study used structural equation modeling to examine the role of teachers' practices and beliefs in implementing differentiated instruction in physical education. Using a sample of 527 preservice teachers, we found that a growth mindset was the strongest predictor of differentiated instruction, followed by flexible grouping and differentiating the curriculum. In addition, multiple mediation analyses revealed that differentiating the curriculum and flexible grouping mediated the relationship between a growth mindset and differentiated instruction. We also guide teacher educators to develop teachers' growth mindsets and help teachers better understand differentiated instruction in physical education.