bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of Plant-Parasitic Nematodes on Seashore Paspalum (Paspalum vaginatum) in Florida
Authors: Hixson A. C. , Crow W. T. 
Published: May 2007

Seashore paspalum (Paspalum vaginatum Swartz) is a warm-season grass capable of growing in the saline environments present in coastal areas of Florida (2). A major limitation of cultivating turfgrasses in the sandy soils of Florida is the destruction of roots by plant-parasitic nematodes (3). A survey was performed to determine the plant-parasitic nematodes associated with seashore paspalum. Sampling locations ranged from Daytona Beach to Miami Beach on the eastern coast and from Tampa to Naples on the western coast. Soil samples were taken during the spring and summer months of 2002 and 2003 from different golf courses and home lawns. In 2002, soil samples were taken from five golf courses (37 samples) and seven home lawns (17 samples). In the next year, three golf courses (23 samples) and 13 home lawns (34 samples) were sampled. Nematodes were extracted from 100 cm3 soil samples using a modified centrifugal-sugar flotation technique (1). Ten genera of plant-parasitic nematodes were present from the samples obtained from golf courses. In addition, two more plant-parasitic nematode genera were present in samples from home lawns. The genera most frequently detected were Hoplolaimus, Mesocriconema, Hemicriconemoides, and Helicotylenchus, which were found at 100, 100, 88, and 88% of the golf courses surveyed and at 75, 95, 70, and 85% of the home lawns sampled, respectively. Genera Xiphinema, Pratylenchus, and Tylenchorhynchus, were found in less than 30% of the golf courses and less than 45% of the home lawns sampled. Genera Peltamigratus and Hemicycliophora were associated with a low percentage of the home lawns. A moderately high frequency of the genus Belonolaimus present in soil samples from golf courses (50%) and home lawns (40%) was consistent for other grasses grown in sandy soils associated with coastal areas in Florida (4). Populations of the genera Belonolaimus, Hoplolaimus, Helicotylenchus, Trichodorus, Hemicriconemoides, and Mesocriconema were above the action threshold levels for bermudagrass used by the University of Florida Nematode Assay Laboratory. Genera Hoplolaimus, Belonolaimus, and Trichodorus were associated with irregular-shaped yellowing and declining turfgrass areas sampled in this survey. Large populations of Helicotylenchus spp. (>500 nematodes per 100 cm3 of soil) were often found associated with seashore paspalum. To our knowledge, this is the first report of plant-parasitic nematodes associated with seashore paspalum in Florida. References: (1) W. R. Jenkins. Plant Dis. Rep. 48:692, 1964. (2) J. Morton. Proc. Fla. State Hortic. Soc. 86:482, 1973. (3) V. G. Perry and H. Rhoades. Pages 144–149 in: Nematology in the Southern Region of the United States. Southern Cooperative Series Bull. 276, University of Arkansas Agric. Pub., Fayetteville, 1982. (4) R. T. Robbins and K. R. Barker. J. Nematol. 6:1, 1974.
First Detection of Phytoplasmas in Rhododendron in the Czech Republic
Authors: Mertelik J. , Kloudova K. , Vanc P. , Mokra V. , Sediva J. , Navratil M. , Valova P. 
Published: May 2007

A 10-year-old plant of rhododendron (‘Cunningham's White’ PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infection was detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII group) as determined previously (2). To our knowledge, this is the first finding of stolbur-type phytoplasma in rhododendron worldwide. References: (1) R. Fialova et al. Plant Prot. Sci. 39(1):7, 2003. (2) I.-M Lee et al. Int. J. Syst. Bacteriol. 48:1153,1998.
Bacterial Canker Caused by Clavibacter michiganensis subsp. michiganensis on Greenhouse-Grown Tomato in the Western Mediterranean Region of Turkey
Authors: Basim E. , Basim H. , Dickstein E. R. , Jones J. B. 
Published: May 2007

During the 2003 winter growing season, a disease occurred on tomato (Lycopersicon esculentum L.) cvs. Selin, Astona, 198 RN, Malike, and Tulin that were growing in almost all greenhouses of Serik, Aksu, Kumluca, Demre, and Kinik in the Antalya Province, and Keçiborlu, Çandir, and Şeyhler in the Isparta Province in the western Mediterranean Region of Turkey. Disease incidence ranged from 26 to 65%, resulting in economically heavy losses. Symptoms were water-soaked, dark brown-to-black lesions on the leaf margins and asymmetrical wilting of the leaflets. In advanced stages of disease, vascular tissue had a light brown discoloration. A gram-positive bacterium was consistently isolated and formed light gray colonies with internal light gray flecks approximately 2 to 3 mm in diameter on the modified semiselective medium (SCM) (2). Thirty isolates were identified as Clavibacter michiganensis subsp. michiganensis using polymerase chain reaction (PCR) with primers CMM 5 and CMM 6 on the basis of consistent amplification of a 614-bp DNA fragment (1). The isolates were also identified as C. michiganensis subsp. michiganensis (similarity 0.810 to 0.888%) using fatty acid methyl ester (FAME) analysis and Sherlock Microbial Identification System software (Microbial ID, Newark, DE). A pathogenicity test was done by spraying a 6-week-old tomato seedling (cv. Selin) with the bacterial concentration of 108 CFU/ml in sterile water. Control tomato seedlings were only sprayed with sterile water. The inoculated plants were covered with polyethylene bags for 48 h and kept in a growth chamber at 25°C. Symptoms developed on the inoculated plants within approximately 10 days and were similar to those observed in the greenhouse. No symptoms developed on control plants. The bacterium was reisolated from inoculated plants, and its identity was confirmed by colony type on SCM, PCR, and FAME analysis. Although it has been known to be present previously in Aegean, eastern Mediterranean, and the eastern Anatolia regions of Turkey, to our knowledge, this is the first report of bacterial canker on greenhouse tomatoes in the western Mediterranean Region of Turkey. Occurrence of the disease in almost all greenhouses may be the result of the high relative humidity present and the lack of protective bactericide applications in many greenhouses of the region. References: (1) J. Dreier et al. Phytopathology 85:462, 1995. (2) M. Fatmi and N. W. Schaad. Phytopathology 78:121, 1988.
First Report of Leaf Spot Caused by Curvularia affinis on Festuca arundinacea in Hubei, China
Authors: Huang J. B. , Zheng L. , Hsiang T. 
Published: May 2007

Curvularia leaf spot affects many species of grasses worldwide but has not been previously reported from central China. In October 2003, symptoms were observed on Festuca arundinacea in a city park in Yichang, Hubei. Fungicides were not used at this site. Leaf spots formed on the foliage after a few days of rainfall. In the most heavily infected areas, the incidence of disease was one leaf in five with spots. Overall, there was an average of 1 leaf in 50 with spots. Infected leaves most commonly bore a single spot, but some leaves had as many as 10 small spots. The spots were round, 5 to 10 mm across, and gray brown in the center with a dark margin. The samples were brought to the lab, and isolations were made on acidified potato dextrose agar (PDA; 3% acetic acid). After 3 days at room temperature, 10-mm-diameter white cultures with appressed mycelium were observed. Two days later, the colonies acquired a greenish cast particularly at the centers, and these yielded spores were identified as Curvularia affinis according to Ellis (1). The key characteristics were a scarcely protuberant hilum, spore size of 25 × 11 μm, and four-septate, curved conidia. DNA sequencing of the internal transcribed spacer region of ribosomal DNA was used to confirm the identity, and aside from sequence length differences, the match with a C. affinis sequence in GenBank was 100%. Inoculating the fungus onto field-grown F. arundinacea that had been washed, cut, and placed in 15-cm-diameter glass petri plates tested pathogenicity. The stems were at least 10 cm long and bore multiple attached leaves. The plates were layered with tissue paper that had been wetted and autoclaved. Sterile water was added to ensure sufficient moisture, and the tissue paper was folded over the bottom 1 cm of stems. Growing the fungus on PDA produced inoculum. Of the eight stems in the plate, one-half were used as a noninoculated check, while the remaining four stems were inoculated with three 5-mm-diameter agar plugs per stem. The plates were incubated at 25°C under continuous fluorescent light, and the inoculum plugs were removed after 48 h. Within 24 h after plug removal, lesions began to appear on the leaves. Circular brown spots were initially less than 5 mm wide, but within 2 days they darkened and grew to 10 mm wide and some of the lesions had a bright yellow margin. No lesions were found on noninoculated stems, which remained turgid and green throughout the trial. C. affinis was successfully reisolated from symptomatic tissue, completing Koch's postulates. To our knowledge, this is the first report of C. affinis on a turfgrass species, although it has been previously found on rice (1). Reference: (1) M. B. Ellis. Dematiaceous hyphomycetes VII: Curvularia, Brachysporium, etc. Mycol. Pap. 106:1, 1966.
Prevalence and clinicopathologic findings of antiphospholipid syndrome nephropathy in Thai systemic lupus erythematosus patients who underwent renal biopsies
Authors: CHEUNSUCHON BOONYARIT , RUNGKAEW PIMPATTANA , CHAWANASUNTORAPOJ RATANA , PATTARAGARN ANIRUT , PARICHATIKANOND PAISAL 
Published: May 2007

SUMMARY:Aim:  To determine the prevalence of antiphospholipid syndrome nephropathy (APSN) in Thai systemic lupus erythematosus (SLE) patients who underwent renal biopsy and to compare the relationship of renal histopathology and other significant clinical parameters between SLE patients with and without APSN.Methods:  A retrospective analysis was undertaken in systemic lupus erythematosus patients (n = 150, 44 <15 years old, 106 0e;15 years old) who underwent renal biopsy. The specimens were evaluated for histological features of APSN and other significant clinical parameters. The result of antiphospholipid antibodies, clinical course, and renal function from chart review were analysed.Results:  The prevalence of APSN in systemic lupus erythematosus patients who underwent renal biopsies was 34% (16% in <15‐year‐old group, 41.5% in 0e;15‐year‐old group). APSN was associated with more severe hypertension (P = 0.002 for systolic and P = 0.004 for diastolic blood pressure), acute renal failure (P = 0.003), persistent heavy proteinuria (P < 0.001 for 4+ proteinuria), severe lupus nephritis (class III and IV, P = 0.014, high activity and chronicity indices, P < 0.001) and a tendency to progress to end‐stage renal disease.Conclusion:  Systemic lupus erythematosus patients who underwent renal biopsies in our institute showed a prevalence of APSN comparable to those in western countries. The presence of APSN was significantly higher in the adult than in the paediatric population. Its association with poor prognostic indicators suggests poor renal outcome. Clinicians should be aware of this condition in order to give proper care to systemic lupus erythematosus patients.
Colonoscopy in elderly Asians: A prospective evaluation in routine clinical practice
Authors: MA Wee‐Tah , MAHADEVA Sanjiv , KUNANAYAGAM Soraya , POI Philip Jun‐Hua , GOH Khean‐Lee 
Published: May 2007

OBJECTIVE:  Colonoscopy is believed to be more complicated in elderly patients in Western countries. It is uncertain if the situation holds true among Asians. This study is to determine differences in colonoscopy performance and sedation complications between patients aged <65 years and those ≥65 years of age in an Asian population.METHODS:  A prospective, cross‐sectional study of adults attending outpatient colonoscopy at a tertiary institution. Clinical and endoscopic data were obtained from all consenting adults.RESULTS:  Two hundred and one patients (70 elderly and 131 aged <65 years) were enrolled. Compared to the patients aged <65 years, the elderly patients had similar levels of good (42.9%vs 45.8%), satisfactory (42.9%vs 33.6%) and poor (14.3%vs 20.6%) bowel preparations (P = NS). Cecal intubation was achieved in 60 (85.7%) of the elderly patients and 116 (88.5%) of the younger adults (P = NS). The differences in mean total colonoscopy duration was not significant (30 ± 13 vs 27 ± 11 min). Although the elderly patients received lower mean sedation doses of midazolam (4.7 vs 5.1 mg) and pethidine (37.8 vs 46.4 mg) compared to the younger adults, the hypotension rates were significantly higher in the elderly patients (7.1%vs 0.8%, P = 0.01). The elderly patients had in additional one or more co‐morbid illnesses (P = 0.001), with significantly higher rates of diabetes (P = 0.004), ischemic heart disease (P = 0.03), hypertension (P = 0.001) and stroke disease (P = 0.004).CONCLUSION:  Colonoscopy performance in elderly Asians is similar to that in younger adults. However, the conscious sedation of these patients results in a higher rate of cardio‐vascular complications.
BEFORE THE ORIGINAL POSITION: The Neo‐Orthodox Theology of the Young John Rawls
Authors: Gregory Eric 
Published: May 2007

ABSTRACTThis paper examines a remarkable document that has escaped critical attention within the vast literature on John Rawls, religion, and liberalism:Rawls'sundergraduate thesis, “A Brief Inquiry into the Meaning of Sin and Faith: An Interpretation Based on the Concept of Community” (1942). The thesis shows the extent to which a once regnant version of Protestant theology has retreated into seminaries and divinity schools where it now also meets resistance. Ironically, the young Rawls rejected social contract liberalism for reasons that anticipate many of the claims later made against him by secular and religious critics. The thesis and Rawls's late unpublished remarks on religion and World War II offer a new dimension to his intellectual biography. They show the significance of his humanist response to the moral impossibility of political theology. Moreover, they also reveal a kind of Rawlsian piety marginalized by contemporary debates over religion and liberalism.
Micro‐environment of olive ridley turtle nests deposited during an aggregated nesting event
Authors: Clusella Trullas S. , Paladino F. V. 
Published: May 2007

AbstractThe hatching success of nests deposited by olive ridley turtles Lepidochelys olivacea during aggregated nesting events (‘arribada’) is typically low and the underlying mechanisms are not clear. In this study, temperature, oxygen and carbon dioxide partial pressures (PO2 and PCO2) of in situ nests as well as nests relocated into a hatchery with clean sand were monitored throughout incubation. Hatching success of hatchery nests was significantly higher than in situ nests (83.1 vs. 21.6%) and mainly resulted from higher mortality of early‐stage embryos. During the first half of incubation, temperature and PCO2 were higher (by 0.6°C and 0.7 kPa, respectively) and PO2 was lower (by 1.1 kPa) within in situ relative to hatchery nests. Because embryo metabolism does not interfere significantly with nest gas contents during the first half of incubation, these results suggest that the greater content of organic matter and/or microorganisms in the sand surrounding in situ nests had an effect on nest gas contents. As PO2 and PCO2 differences were relatively small, microbial activity (such as fungal and bacterial infection) may have caused the early embryo mortality found in situ. Moreover, our results suggest that during the second half of incubation, neither PO2 nor PCO2 reached threshold levels that resulted in the death of embryos or hatchlings. Overall, this study showed a clear benefit of using clean sand to increase hatchling production in arribada beaches and highlights the importance of further investigating the relationship between nest micro‐environment, sand microbial activity and embryo development under natural conditions during these unique nesting events.
Tracks for traffic: microtubules in the plant pathogen Ustilago maydis
Authors: Steinberg Gero 
Published: May 2007

SummaryPathogenic development of the corn smut fungus Ustilago maydis depends on the ability of the hypha to grow invasively. Extended hyphal growth and mitosis require microtubules, as revealed by recent studies on the microtubule cytoskeleton. Surprisingly, hyphal tip growth involves only two out of 10 kinesins. Kinesin‐3 is responsible for tip‐directed (anterograde) endosome motility of early endosomes, which are thought to support hyphal elongation by apical membrane recycling. In addition, kinesin‐3, together with kinesin‐1 and myosin‐5, appear to deliver secretory vesicles to the hyphal tip. Kinesin‐1 also affects endosome motility by targeting cytoplasmic dynein to microtubule plus ends. This plus‐end localization of dynein is essential for cell body‐directed (retrograde) endosome motility, but also allows force generation during spindle elongation in mitosis. Furthermore, kinesin‐1 and dynein participate in the organization of the microtubule array, thereby building their own network of tracks for intracellular motility. The recent progress in understanding microtubule‐based processes in U. maydis has revealed an unexpected complexity of motor functions essential for the virulence of this pathogen. Further studies on structural and regulatory requirements for motor activity should help identify novel targets for fungicide development.

Contents

Summary
721

I.
Introduction
722

II.
Organization of the interphase microtubule array in Ustilago maydis
724

III.
Molecular motors in hyphal tip growth of Ustilago maydis
727

IV.
Mechanisms of mitosis
729

V.
Questions and future perspectives
730

Acknowledgements
730

References
731
Polymorphic microsatellite markers for chromosomal races of Australian morabine grasshoppers (Vandiemenella, viatica species group)
Authors: KAWAKAMI T. , BUTLIN R. K. , PAULL D. J. , COOPER S. J. B 
Published: May 2007

AbstractChromosomally diverse Australian morabine grasshoppers (genus Vandiemenella, viatica species group) have parapatric distributions and occasionally hybridize at contact zones. To investigate population genetic structure and the extent of gene flow between chromosomal races/species of Vandiemenella, we isolated and characterized nine polymorphic microsatellite loci and one insertion/deletion polymorphic locus. The numbers of alleles per locus ranged from two to 34 across three chromosomal races on Kangaroo Island, South Australia, and expected heterozygosity within races ranged from 0.00 to 0.94. Inter‐taxon amplification was generally successful within Vandiemenella, but not for other morabine genera.
Use of dopamine in acute renal failure
Authors: Sigrist Nadja E. 
Published: May 2007

AbstractObjective:To review the current understanding of dopamine and its use in the prevention and treatment of acute renal failure (ARF).Data sources:Original research articles and scientific reviews.Human data synthesis:Low‐dose dopamine administration has been shown to increase natriuresis and urinary output in both healthy individuals and in a few small studies in human patients with renal insufficiency. However, in several large meta‐analyses, dopamine treatment did not change mortality or the need for dialysis. Due to the potential side effects, the use of dopamine for prevention and treatment of ARF is no longer recommended in human medicine.Veterinary data synthesis:Low‐dose dopamine increases urinary output in healthy animals and animal models of ARF if given before the insult. There are no available studies looking at the effect of low‐dose dopamine therapy in naturally occurring ARF in dogs or cats.Conclusion:Due to the potential side effects of low‐dose dopamine therapy, the results from large human trials, and the lack of information in veterinary medicine, the use of dopamine for treatment of ARF in veterinary patients should be further evaluated.
Evaluation of adding diltiazem therapy to standard treatment of acute renal failure caused by leptospirosis: 18 dogs (1998–2001)
Authors: Mathews Karol A. , Monteith Gabrielle 
Published: May 2007

AbstractObjective:To assess efficacy and safety of intravenous (IV) diltiazem as a treatment for acute renal failure (ARF) secondary to leptospirosis in dogs.Design:Retrospective studyAnimals:Eighteen dogs with ARF caused byLeptospira spptreated during the months of September to December (1998–2001).Procedure:All dogs treated for ARF caused byLeptospira sppwere enrolled in the study and were treated with standard care consisting of IV fluids, +/− furosemide, and antibiotics. With owner consent some dogs were treated with diltiazem at 0.1–0.5 mg/kg (0.045–0.22 mg/lb) IV slowly, followed by 1–5 μg/kg/minutes (0.45–2.2 mg/lb/minutes) constant rate infusion. Outcome measures were compared between the 2 groups (diltiazemversusstandard). Diltiazem was administered within 60 hours of admission until serum creatinine fell into the normal range or stabilized. The primary outcome measurement of safety was systolic blood pressure (SBP). The primary measurement of efficacy outcome was the rate and magnitude of reduction of serum creatinineResults:Eleven out of 18 dogs received diltiazem. The rate of reduction in creatinine in the diltiazem group was 1.76 times faster than the standard group (P=0.054). Recovery of renal function showed a trend towards significant association with treatment group (exactP=0.08, odds ratio=3.62). This effect may be clinically relevant. Diltiazem had no clinically relevant effect on SBP.Conclusions and clinical relevance:Renal recovery in dogs with acute renal failure secondary to leptospirosis is improved with the administration of diltiazem in addition to ‘standard’ therapy.