bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salinity Effects on Growth and Nutritional Content of Newly Isolated Microalgal with Potential Use in The Shrimp-Hatcheries
Authors: Iba Wa , Balubi Abdul Muis Balubi , Martin Lenore , A. Rice Michael , H. Wikfors Gary 
Published: April 2024

A two-week batch experiment was conducted on three newly isolated Indonesian microalgal strains (Kb1-2 identified as Chaetoceros sp., Kb1-3 and Kb1-5) and Tisochrysis lutea to determine salinity effects upon the growth, proximate composition and ω-3, eicosapentaenoic acidand docosahexaenoic acid, (EPA and DHA) and ω-6 (arachidonic acid /ARA) fatty acids. Salinity within each strain growth of all microalgae tested. The highest cell densities were observed in Indonesian strains, Kb1-3 on day 8 at 25 psu and Kb1-5 on day 10 at 35 psu. Salinity significantly affected the lipid, protein and carbohydrate content in all microalgae cultured. The highest total lipid content was found in T. lutea cultured at 30 psu (28.3 %) followed by Kb1-2 cultured at 20 psu (25.0 %) and T. lutea at 35 psu (24.8 %). Kb1-3 produced highest protein when cultured at 20 and 25 psu, decreasing at higher salinities of 30 and 35 psu, 44.7 and 39.2 % to 31.5 and 32.6 %, respectively, similar to T. lutea. Kb1-5 had higher protein at both 25 and 35 psu but showed lower protein levels at 20 and 30 psu. Indonesian strains showed almost a similar content of carbohydrate across culture salinities similar to T. lutea. Although all Indonesian microalgae contained important ω-3 (EPA and DHA) and ω-6 (ARA) fatty acids, concentrations were low in comparison to T. lutea. All Indonesian microalgal strains also contained the dicarboxylic acid (DCA), phthalic acid, which was not present in T. lutea.
Increasing the Non-Specific Immunity of Vannamei Shrimp (Litopenaeus vannamei) Maintained in Traditional Ponds with the Addition of Fitoimun®
Authors: Rahwara Munawwarah , Hardi Esti Handayani , Septiani Gina , Sulistyawati Sulistyawati , Maidie Asfie 
Published: April 2024

Vannamei shrimp (Litopenaeus vannamei) is a commodity that is in great demand by the community. The purpose of this study were to examine the use of Fitoimun® mixed in feed to increase non-specific immunity, survival the morphology of vannamei shrimp. This study used a completely randomized design, with 3 treatments and 3 replications. The Fitoimun® product used made from extracts of fingerroot (Boesenbergia pandurata) and tamarind eggplant (Solanum ferox) was mixed with pellet feed. The doses used in the treatment were 0 mL/kg, 20 mL/kg, 24 mL/kg. The feed was given twice a day, in the morning and evening as much as 500 grams per repetition. The research was conducted for 3 months and observations were carried out for one month. The shrimp was purchased from the Brackish and Sea Water Central Seed Center (BBU) manggar Balikpapan with an average weight of 27 g. Sampling was carried out once a week. Haemolymph was taken for the observation of immunity. The results showed that the administration of Fitoimun® increased non-specific immunity such as total haemocytes with the highest value of 125.7 cells/mL and differential haemocytes with the highest value in granulocyte cells 43.90%, semi granulocyte cells 42.57% and hyaline 32.63 %. The Survival were 52% and there was no significant difference between treatments. Fitoimun® did not affect the morphology of Pasific whiteleg shrimp.
Prediction of the discharge capacity of piano key weirs using artificial neural networks
Authors: Iqbal Mujahid , Ghani Usman 
Published: April 2024

ABSTRACT
The discharge capacity of the piano key weir (PKW) is an important flow feature which ultimately decides the design of PKWs. In the present research work, the different architecture of artificial neural networks (ANNs) was employed to predict the discharge capacity of the trapezoidal piano key weir (TPKW) by varying geometric parameters. Furthermore, adaptive neuro-fuzzy interference system (ANFIS), support vector machines (SVMs) and non-linear regression (RM) techniques were also applied to compare the performance of best ANN models. The performance of each model was evaluated using statistical indices including scatter-index (SI); coefficient of determination (R2) and mean square error (MSE). The prediction capability of all the models was found to be satisfactory. However, results predicted by ANN-22(H-15) [R2 = 0.998, MSE= 0.0024, SI = 0.0177] were more accurate than ANFIS (R2 = 0.995, MSE = 0.00039, SI=0.0256), SVM (R2 = 0.982, MSE = 0.000864, SI =0.0395) and RM (R2 = 0.978, MSE = 0.001, SI = 0.0411). It was observed that Si/So, Wi/Wo and L/W ratios have the greatest effect on the discharge performance of TPKW. Furthermore, sensitivity analysis confirmed that h/P is the most influencing ratio which may considerably affect the discharge efficiency of the TPKW and ANN architecture having a single hidden layer and keeping neurons three times of input parameters in hidden layers generated better results.
Celastrol Elicits Antitumor Effects through Inducing Immunogenic Cell Death
and Downregulating PD-L1 in ccRCC
Authors: Li Hong-Fang , Zhu Neng , Wu Jia-Jun , Shi Ya-Ning , Gu Jia , Qin Li 
Published: April 2024

Background::
Targeting immunogenic cell death (ICD) is considered a promising therapeutic strategy
for cancer. However, the commonly identified ICD inducers promote the expression of programmed cell
death ligand 1 (PD-L1) in tumor cells, thus aiding them to evade the recognition and killing by the immune
system. Therefore, the finding of novel ICD inducers to avoid enhanced PD-L1 expression is of vital significance
for cancer therapy. Celastrol (CeT), a triterpene isolated from Tripterygium wilfordii Hook. F induces
various forms of cell death to exert anti-cancer effects, which may make celastrol an attractive candidate as an
inducer of ICD.

Methods::
In the present study, bioinformatics analysis was combined with experimental validation to explore
the underlying mechanism by which CeT induces ICD and regulates PD-L1 expression in clear cell renal cell
carcinoma (ccRCC).

Results::
The results showed that EGFR, IKBKB, PRKCQ and MAPK1 were the crucial targets for CeT-induced
ICD, and only MAPK1 was an independent prognostic factor for the overall survival (OS) of ccRCC patients.
In addition, CeT triggered autophagy and up-regulated the expressions of HMGB1 and CRT to induce
ICD in 786-O cells in vitro. Importantly, CeT can down-regulate PD-L1 expression through activating autophagy.
At the molecular level, CeT suppressed PD-L1 via the inhibition of MAPK1 expression. Immunologically,
the core target of celastrol, MAPK1, was tightly correlated with CD8+ T cells and CD4+ T cells in ccRCC.

Conclusion::
These findings indicate that CeT not only induces ICD but also suppresses PD-L1 by down-regulating
MAPK1 expression, which will provide an attractive strategy for ccRCC immunotherapy.
ERH Impacts Patient Prognosis and Tumor Immune Microenvironment: A Pan-Cancer Analysis
Authors: Gong Qianhui , Li Qiong , Xu Zhichao , Shen Xiaobing 
Published: April 2024

Background:
The enhancer of rudimentary homolog (ERH) has been shown to play
significant roles in tumorigenesis and progression. However, few systematic pan-cancer analyses
about ERH have been described.

Methods:
From the tumor immune estimation resource web server2.0 (TIMER2.0), the Genotype-
Tissue Expression database (GTEx) and the Gene Expression Profile Interactive Analysis
version 2 (GEPIA2) databases, we explored the expression profiles and prognostic significance
of ERH in 33 cancers. The Clinical Proteomic Tumor Analysis Consortium (CPTAC) and the
Human Protein Atlas (HPA) databases were further used to examine the differential expression
of ERH at the protein level. The genetic alteration profile was obtained from the cBioPortal. The
correlation between ERH expression and the quantities of immune infiltrating cells was examined
by the TIMER tool. Spearman's correlation test was conducted to analyze the association
between ERH expression status and a number of prognostic indicators, including immune checkpoints,
TMB, MSI, immune neoantigen, MMR genes, and DNA methyltransferases. Protein-
Protein Interaction analyses were performed in the String and GeneMANIA databases, and enrichment
analysis and predicted signaling pathways were identified through GO and KEGG. To
make our results convincing, we validated them in six datasets in the Gene Expression Omnibus
(GEO) database. In addition, we verified the expression of ERH between gastric cancer tissues
and adjacent normal tissues by RT-qPCR.

Results:
ERH expression was elevated in numerous tumors, and it was associated with the patient's
prognosis. Furthermore, the quantities of immune infiltrating cells and immune checkpoint
genes were remarkably associated with ERH. TMB and MSI were related to ERH expression in
14 and 15 cancer types, respectively. Moreover, the expression of ERH was strongly associated
with MMR defects in multiple cancer types, and almost all tumors showed co-expression of ERH
and four DNA methyltransferases. The results of GO and KEGG analysis confirmed that ERH
potentially impacts several important signaling pathways. Both the GEO datasets and the RTqPCR
experiment validated our previous analysis.

Conclusion:
Our pan-cancer analysis demonstrated the characterization of ERH in multiple tumors.
ERH may be a valuable novel biological indicator for assessing immunotherapy efficacy
and prognosis in various malignancies.
Antimicrobial Potential of Natural Compounds of Zingiberaceae Plants and their Synthetic Analogues: A Scoping Review of In vitro and In silico Approaches
Authors: Yit Kok-Hou , Zainal-Abidin Zamirah 
Published: April 2024

Aim::
There has been increased scientific interest in bioactive compounds and their synthetic
derivatives to promote the development of antimicrobial agents that could be used sustainably
and overcome antibiotic resistance.

Methods::
We conducted this scoping review to collect evidence related to the antimicrobial potential
of diverse natural compounds from Zingiberaceae plants and their synthetic derivatives. We
followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Extension
for Scoping Reviews guidelines. The literature search was conducted using PubMed, Web
of Science and Scopus electronic databases for relevant studies published from 2012 to 2023. A total
of 28 scientific studies fulfilled the inclusion criteria. The authors of these studies implemented
in vitro and in silico methods to examine the antimicrobial potency and underlying mechanisms of
the investigated compounds.

Result::
The evidence elucidates the antimicrobial activity of natural secondary metabolites from
Zingiberaceae species and their synthetic derivatives against a broad panel of gram-positive and
gram-negative bacteria, fungi and viruses.

Conclusion::
To date, researchers have proposed the application of bioactive compounds derived
from Zingiberaceae plants and their synthetic analogues as antimicrobial agents. Nevertheless,
more investigations are required to ascertain their efficacy and to broaden their commercial applicability.
Development and Outlook for Pipeline Inspection Robots
Authors: Zhang Yuan , Yang Yupeng , Lu Shuai , Zhou Enwen 
Published: April 2024

Background:
Robots for pipeline inspection are frequently employed in long-distance oil,
wastewater, and natural gas pipelines. Much complex research is being conductedon pipeline inspection
robots, including work on fluid-driven and non-fluid-driven, among other things. Research on
pipeline inspection robots can be advanced by examining their current stage of development. Additionally,
pipeline inspection robots may be able to promote the growth of the pipeline transportation
sector by creating new structures.

Objective:
This study aims to examine the benefits and drawbacks of several pipeline inspection
robots and theirdevelopment trend through the lens of the most recent advancements in the field.

Methods:
Through the structure and application scenarios of the latest representative patents of pipeline
inspection robots, the working principle and characteristics of pipeline inspection robots are
demonstrated.

Results:
The shortcomings of the current pipeline inspection robots are highlighted, along with potential
future development pathways and research issues, by comparing pipeline inspection robots of
various structures.

Conclusion:
The development of pipeline inspection robots has benefited the construction of sewage,
oil, natural gas, and long-distance pipelines, as well as long-distance pipeline transportation.
Because of these robots' unique features and ample room for advancement, pipeline inspection robots
have even more development prospects.
Cadenas regionales de valor en alimentos procesados en la Alianza del Pacífico
Authors: Gómez Parada Edgar Javier , González Escobar Jimmy , González Polo Gyseidys 
Published: April 2024

Objetivo: el presente estudio se enfocó en identificar las oportunidades de encadenamientos productivos para la conformación de cadenas regionales de valor en el sector de alimentos procesados entre Chile, Colombia, México y Perú, países que conforman la Alianza del Pacífico (AP).
Metodología: se aplicó una metodología cualitativa. Para esto, se seleccionaron productos del sector de alimentos que presentaron saldo comercial positivo para algunos de los países de la AP, entre los años 2017-2021, identificándose, dentro de ellos, bienes finales e insumos según los procesos de elaboración industrial y se organizaron cadenas de valor; posteriormente, se estableció el papel que desempeña cada país como fabricante de bienes finales o como proveedor de insumos en la cadena. Los productos se asociaron a la clasificación del sistema armonizado arancelario a cuatro dígitos.
Resultados: Se identificaron trece cadenas de valor asociadas a los bienes finales y treinta y dos bienes insumos con potencial exportador; se destaca que los cuatro países pueden ser fabricantes de bienes finales en al menos tres cadenas; México puede ser fabricante de bienes finales en doce cadenas; con respecto a insumos, en siete de las trece cadenas pueden participar los cuatro países de la Alianza conjuntamente; en las demás, participan al menos tres de ellos.
Conclusiones: se aprecia que los países de la Alianza del Pacífico tienen potencial para conformar cadenas de valor en alimentos procesados; se destaca, además, el liderazgo que en esta industria tiene México como fabricante de bienes finales, así como la diversidad de insumos que puede suministrar cada país.
Originalidad: sobresale el papel que puede desempeñar cada país de la Alianza del Pacífico como fabricante de productos finales o proveedores de insumos en los productos que tienen fortalezas exportadoras hacia la conformación de articulaciones productivas.
Preoperative parameters (signalment, digital radiography, urinalysis, urine microbiological culture) and novel algorithm improve prediction of canine urocystolith composition
Authors: To Iris , Berent Allyson C. , Weisse Chick W. , An Anjile , Harling Brett , Sack Danny , Ciardullo Robert , Slade Dennis J. , Palma Douglas A. , DeJesus Antonia A. , Fischetti Anthony J. 
Published: April 2024

Abstract

OBJECTIVE
To determine the accuracy of 4 preoperative parameters (signalment, urinalysis, urine microbiological culture, and digital radiography) in predicting urocystolith composition, compare accuracy between evaluators of varying clinical experience and a mobile application, and propose a novel algorithm to improve accuracy.

ANIMALS
175 client-owned dogs with quantitative analyses of urocystoliths between January 1, 2012, and July 31, 2020.

METHODS
Prospective experimental study. Canine urocystolith cases were randomly presented to 6 blinded “stone evaluators” (rotating interns, radiologists, internists) in 3 rounds, each separated by 2 weeks: case data alone, case data with a urolith teaching lecture, and case data with a novel algorithm. Case data were also entered into the Minnesota Urolith Center mobile application. Prediction accuracy was determined by comparison to quantitative laboratory stone analysis results.

RESULTS
Prediction accuracy of evaluators varied with experience when shown case data alone (accuracy, 57% to 82%) but improved with a teaching lecture (accuracy, 76% to 89%) and further improved with a novel algorithm (accuracy, 93% to 96%). Mixed stone compositions were the most incorrectly predicted type. Mobile application accuracy was 74%.

CLINICAL RELEVANCE
Use of the 4 preoperative parameters resulted in variable accuracy of urocystolith composition predictions among evaluators. The proposed novel algorithm improves accuracy for all clinicians, surpassing accuracy of the mobile application, and may help guide patient management.
Systemic absorption and gastrointestinal adverse effects from topical ketorolac and diclofenac ophthalmic solutions in healthy dogs
Authors: Van Vertloo Laura R. , Sebbag Lionel , Allbaugh Rachel A. , Allenspach Karin , Borts David J. , Mochel Jonathan P. 
Published: April 2024

Abstract

OBJECTIVE
To investigate systemic absorption and gastrointestinal (GI) adverse effects of topical ketorolac 0.5% and diclofenac 0.1% ophthalmic solutions.

ANIMALS
11 healthy purpose-bred Beagles.

METHODS
Dogs were randomly assigned to receive either ketorolac (n = 6) or diclofenac (5), 1 drop in both eyes 4 times daily for 28 days. Upper GI endoscopy was performed on days 0 and 29 with mucosal lesion scores (0 to 7) assigned to each region evaluated. Plasma samples were collected on days 14, 21, and 28 for measurement of diclofenac and ketorolac using high-performance liquid chromatography–mass spectrometry.

RESULTS
GI erosions and/or ulcers developed in all ketorolac-treated dogs and 1 of 5 diclofenac-treated dogs. Post-treatment mucosal lesion score for the antrum was higher in the ketorolac group than in the diclofenac group (P = .006) but not significantly different for any other region. Post-treatment antral mucosal lesion scores were significantly related to plasma ketorolac concentrations (P < .001). Ketorolac and diclofenac were detected in the plasma at all time points (median ketorolac day 14, 191 ng/mL; day 21, 173.5 ng/mL; and day 28, 179.5 ng/mL; and median diclofenac day 14, 21.1 ng/mL; day 21, 20.6 ng/mL; day 28, 27.5 ng/mL). Vomiting and decreased appetite events were observed uncommonly and were not significantly different between treatment groups.

CLINICAL RELEVANCE
GI ulceration and erosion developed after ophthalmic administration of ketorolac and diclofenac, with higher plasma concentrations and more severe GI lesions associated with ketorolac. Clients should be alerted to this potential risk with ophthalmic use and informed to watch for systemic clinical signs that would warrant veterinary reevaluation.
Oscillometric and Doppler techniques can provide continuous noninvasive blood pressure readings in cats and dogs: an instructional video
Authors: Haginoya Satoshi , Thomovsky Elizabeth J. 
Published: April 2024

Abstract

OBJECTIVE
To provide a video tutorial detailing how to perform continuous noninvasive blood pressure monitoring in dogs and cats.

ANIMALS
Any size dog or cat.

METHODS
To measure blood pressure noninvasively, a blood pressure cuff is selected on the basis of the circumference of the limb and placed at the level of the right atrium. For oscillometric blood pressure measurement, the cuff is connected to an oscillometric unit that will automatically inflate and deflate the cuff in order to measure the patient’s blood pressure using an internal algorithm. For Doppler blood pressure measurement, a sphygmomanometer is used to manually inflate the pressure cuff 30 to 40 mm Hg above the point where the audible arterial sounds disappear. Then, the cuff is gradually deflated until the audible arterial sounds return; the pressure at the first sound is recorded as the blood pressure. To generate continuous readings, the oscillometric machine is set to measure blood pressure as often as every minute. Alternatively, the Doppler crystal is taped to the patient’s leg to facilitate repeated cuff inflation/deflation and collection of blood pressure values as often as every minute.

RESULTS
Continuous blood pressure readings can be obtained by both the oscillometric and Doppler techniques.

CLINICAL RELEVANCE
Continuous blood pressure readings identify trends in a patient’s cardiovascular status. The most reliable oscillometric blood pressure reading is the mean arterial pressure. Doppler blood pressure values are considered systolic in dogs. Doppler values in cats underestimate systolic and overestimate mean blood pressure.
Industry 4.0 and Sustainability: a Study on the Applicability of Additive Manufacturing as a Tool for Minimizing Environmental Impacts on Production Processes
Authors: Silva Cássia Mara Alexandrino , Gomes Vitor Carvalho , Ferreira Allan Berthier Silva , Nascimento Leonardo Dias , Leite André Búrigo , Santos Vinicius Saraiva , Acruz Carla Santos , Marques Francisco Roldineli Varela , Leão Airton Pereira da Silva , Tourem Rafael Viêro 
Published: April 2024

Objective: The present research sought to analyze the applicability of additive manufacturing as a tool for minimizing environmental damage in production processes.

Methodology: The research was conducted through an integrative literature review. Academic platforms such as SciELO and Google Academic were consulted, using specific keywords related to the theme. Inclusion criteria were established to select complete articles, in Portuguese and Brazilian languages, directly associated with the research theme, published between 2020 and 2024.

Results and data analysis: The revised studies emphasize the growing importance of additive manufacturing (AM) and 3D printing technology in transforming production processes towards more sustainable and efficient practices. MA's ability to produce complex parts accurately and personalized, significantly reducing raw material waste through layer-by-layer manufacturing, stands out as a crucial tool to minimize environmental impacts on production. In addition, the integration of MA in the early stages of product development promotes efficiency in production, although more in-depth studies on its environmental and energy impacts are needed.

Conclusion: It is therefore concluded that additive manufacturing is an essential tool for more sustainable and innovative practices in the industry, promoting the conservation of natural resources, the reduction of waste and the creation of adaptable and customized solutions. It is therefore up to companies and society to exploit and develop these technologies in search of a more sustainable and competitive future.