bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kindlin-2-mediated hematopoiesis remodeling regulates triple-negative breast cancer immune evasion
Authors: Wang Wei , Chaudhary Rahul , Szpendyk Justin , El Khalki Lamyae , Yousafzai Neelum Aziz , Chan Ricky , Desai Amar , Sossey-Alaoui Khalid 
Published: February 2025

Abstract
Triple-negative breast cancer (TNBC) presents significant clinical challenges due to its limited treatment options and aggressive behavior, often associated with poor prognosis. This study focuses on Kindlin-2, an adaptor protein, and its role in TNBC progression, particularly in hematopoiesis-mediated immune evasion. TNBC tumors expressing high levels of Kindlin-2 induce a notable reshaping of hematopoiesis, promoting expansion of myeloid cells in bone marrow (BM) and spleen. This shift correlated with increased levels of neutrophils and monocytes in tumor-bearing mice over time. Conversely, genetic knockout of Kindlin-2 mitigated this myeloid bias and fostered T cell infiltration within the tumor microenvironment, indicating Kindlin-2's pivotal role in immune modulation. Further investigations revealed that Kindlin-2 deficiency led to reduced expression of PD-L1, a critical immune checkpoint inhibitor, in TNBC tumors. This molecular change sensitized Kindlin-2-deficient tumors to host anti-tumor immune responses, resulting in enhanced tumor suppression in immune-competent mouse models. Single-cell RNA sequencing, bulk RNA-seq, and immunohistochemistry data supported these findings by highlighting enriched immune-related pathways and increased infiltration of immune cells in Kindlin-2-deficient tumors. Therapeutically, targeting PD-L1 in Kindlin-2-expressing TNBC tumors effectively inhibited tumor growth, akin to the effects observed with genetic Kindlin-2 knockout or PD-L1-KO. Our data underscore Kindlin-2 as a promising therapeutic target in combination with immune checkpoint blockade to bolster anti-tumor immunity and counteract resistance mechanisms typical of TNBC and other immune evasive solid tumors. Implications: Kindlin-2 regulates tumor immune evasion through the systemic modulation of hematopoiesis and PD-L1 expression, which warrants therapeutic targeting of Kindlin-2 in TNBC patients.
Strengthening Asian/Asian American, Native Hawaiian, and Pacific Islander Leadership in Cancer Research
Authors: Wu Lawrence W. , Moy Ryan H. ,   the AACR AANHPI Task Force, Bhardwaj Nina , Cho Kathleen , Feng Gen-Sheng , Kim Edward S. , Lei Ming , Liu Edison T. , Maitra Anirban , Mori Motomi , Ng Irene O.L. , Oshima Masanobu , Pao William , Park Ben Ho , Ramalingam Suresh , Rha Sun Young , Ryeom Sandra W. , Shaw Alice T. , Siu Lillian L. , Suri Anil K. , Tan Daniel S. , Tanabe Kenneth K. , Ueno Naoto T. , Verma Sunil , Wong Kwok-Kin , Wu Catherine J. , Wu Hong , Yang James Chih-Hsin 
Published: February 2025

Summary:
Individuals in the United States from Asian and Asian American, Native Hawaiian, and Pacific Islander (AANHPI) backgrounds face a distinct set of cancer-related disparities. In this study, in conjunction with the American Association for Cancer Research Asian/AANHPI Task Force, we highlight the unique disparities faced by AANHPI patients and professionals, and we offer actionable recommendations on how to strengthen AANHPI leadership in cancer research.
A Material Transfer Agreement between Glioblastoma and Normal Brain Cells
Authors: Shakya Sajina , Hubert Christopher G. , Lathia Justin D. 
Published: February 2025

Summary:
Tumor cells communicate with normal cells in various ways, typically leading to the exploitation of resources of the normal cells by tumor cells for their benefit. In this issue, Mangena and colleagues use three-dimensional organoid models to show the transfer of GFP and mRNA from malignant glioblastoma to nonmalignant cells in cerebral organoid models; this transfer is facilitated by extracellular vesicles and possibly tunneling nanotubes, demonstrating how nonmalignant cells in the tumor microenvironment can be exploited by neighboring malignant cells.
See related article by Mangena et al., p. 299
Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
Authors: Sun Jiazheng , Wang Pin , Yi Ziying , Wu Yushen , Wei Yuxian , Fang Huiying , Song Daqiang , Chen Yuru , Du Huimin , Huang Jing , Li Qin , Yang Dejuan , Ren Guosheng , Li Hongzhong 
Published: February 2025

Abstract
The limited infiltration of CD8+ T cells in tumors hampers the effectiveness of T cell–based immunotherapy, yet the mechanisms that limit tumor infiltration by CD8+ T cells remain unclear. Through bulk RNA sequencing of human tumors, we identified a strong correlation between WNT7A expression and reduced CD8+ T-cell infiltration. Further investigation demonstrated that inhibiting WNT7A substantially enhanced MHC-I expression on tumor cells. Mechanistically, WNT7A inhibition inactivated the Wnt/β-catenin signaling pathway and thus resulted in reduced physical interaction between β-catenin and p65 in the cytoplasm, which increased the nuclear translocation of p65 and activated the NF-κB pathway, ultimately promoting the transcription of genes encoding MHC-I molecules. We found that our lead compound, 1365-0109, disrupted the protein–protein interaction between WNT7A and its receptor FZD5, resulting in the upregulation of MHC-I expression. In murine tumor models, both genetic and pharmaceutical suppression of WNT7A led to increased MHC-I levels on tumor cells, and consequently enhanced the infiltration and functionality of CD8+ T cells, which bolstered antitumor immunity and improved the effectiveness of immune checkpoint blockade therapy. These findings have elucidated the intrinsic mechanisms of WNT7A-induced immune suppression, suggesting that therapeutic interventions targeting WNT7A hold promise for enhancing the efficacy of immunotherapy.
Stuttering: Our Current Knowledge, Research Opportunities, and Ways to Address Critical Gaps
Authors: Chang Soo-Eun , Below Jennifer E. , Chow Ho Ming , Guenther Frank H. , Hampton Wray Amanda M. , Jackson Eric S. , Max Ludo , Neef Nicole E. , SheikhBahaei Shahriar , Shekim Lana , Tichenor Seth E. , Walsh Bridget , Watkins Kate E. , Yaruss J. Scott , Bernstein Ratner Nan 
Published: February 2025

Abstract
Our understanding of the neurobiological bases of stuttering remains limited, hampering development of effective treatments that are informed by basic science. Stuttering affects more than 5% of all preschool-age children and remains chronic in approximately 1% of adults worldwide. As a condition that affects a most fundamental human ability to engage in fluid and spontaneous verbal communication, stuttering can have substantial psychosocial, occupational, and educational impacts on those who are affected. This article summarizes invited talks and breakout sessions that were held in June 2023 as part of a 2-day workshop sponsored by the US National Institute on Deafness and Other Communication Disorders. The workshop encompassed topics including neurobiology, genetics, speech motor control, cognitive, social, and emotional impacts, and intervention. Updates on current research in these areas were summarized by each speaker, and critical gaps and priorities for future research were raised, and then discussed by participants. Research talks were followed by smaller, moderated breakout sessions intended to elicit diverse perspectives, including on the matter of defining therapeutic targets for stuttering. A major concern that emerged following participant discussion was whether priorities for treatment in older children and adults should focus on targeting core speech symptoms of stuttering, or on embracing effective communication regardless of whether the speaker exhibits overt stuttering. This article concludes with accumulated convergent points endorsed by most attendees on research and clinical priorities that may lead to breakthroughs with substantial potential to contribute to bettering the lives of those living with this complex speech disorder.
Observing the impact of meal consumption on limb occlusion pressure in Indian female collegiates
Authors: Hariramani M. , Singh G. , Kothiyal S. , Navjyot T. , Sharma N. , Kaur S. , Majhi R. , Prathap G.D. 
Published: February 2025

Abstract
Blood flow resistance training (BFRT), characterised by low load, short duration, and high-efficiency exercises, has gained popularity among coaches, athletes, and health enthusiasts. Proper application of a compressible cuff, determined by limb occlusion pressure (LOP), is essential for effective BFRT. LOP is influenced by several factors, including limb size, length, temperature, blood pressure, and location. The objective of the study was to observe the variance in LOP, before and after meal consumption, in Indian female collegiate and to find the correlation between LOP and other variables. 93 female collegiate athletes participated in this study. LOP of the lower limb was measured pre- and post-meal using a hand-held Doppler, cuff inflator, and occlusion cuff. Data analysis was performed using IBM SPSS version 21. The mean LOP before meals was 66.99 mmHg (95% CI: 57.38-84.98 mmHg) with a median of 260.00 mmHg (interquartile range: 230.00-300.00 mmHg). Post-meal, the mean LOP increased to 69.98 mmHg (95% CI: 58.91-77.74 mmHg), with a median of 230.00 mmHg (interquartile range: 180.00-260.00 mmHg). A significant decrease in LOP after a meal was observed (Z-score: −7.67, ). Higher weight correlated positively with pre-meal LOP (rho = 0.261, ), and BMI also showed a positive correlation with pre-meal LOP (rho = 0.340, ). In conclusion, meal consumption significantly reduces LOP in Indian female collegiates. Additionally, weight and BMI positively correlate with pre-meal LOP, highlighting the need to consider body composition in LOP studies.
“Just a little overweight”
Authors: Alghazo Sharif , Ahmed Mervat M. , Rabab'ah Ghaleb , El-Dakhs Dina Abdel Salam 
Published: February 2025

Abstract
This study investigates the impacts of age and gender on the use of euphemistic strategies in Alexandrian Arabic, a dialect that is spoken in the coastal city of Alexandria to the north-west of Egypt. To achieve this objective, a role-play that includes 6 situations categorised to account for the age and gender variables groups was developed and administered to 160 Alexandrian Arabic speakers, who were divided into four primary groups, each consisting of 40 speakers based on age (old vs young) and gender (male vs female). The responses were analysed using a mixed-methods approach, with quantitative and qualitative measures. The analysis is based on a taxonomy we collected from many previous frameworks, such as Allan and Burridge (1991) and Warren (1992). The results revealed age to be a crucial factor in selecting euphemistic strategies: the older group most frequently employed the implication strategy, and the synonym strategy was more common among the younger group. The results further revealed that gender has a lesser effect on the choice of euphemistic strategies by the participants: borrowing is more common among the female participants, and other methods are utilised almost equally by the two genders. This denotes that although specific euphemism strategies, like synonymy and deletion, can be age-specific, others, such as understatement and taboo, cut across age groups, which can perhaps be seen as manifestations of norms culturally embedded. Gender differences are also only slightly significant except in borrowing, in which females use this strategy more than males, perhaps due to their sociocultural or sociolinguistic functions that shaped women’s language.
A relevance-theoretic approach to weak explicatures as a means to understanding poetic effects
Authors: Arga Bora , Doğan Gürkan 
Published: February 2025

Abstract
What makes the indeterminacy in poetic language so alluring, or at times, frustrating for some readers? How do we take pleasure in not being able to reach an ultimate meaning while reading poetry, in other words, from the impossibility of fully clarifying the message conveyed by the poet? In order to answer these questions and account for cognitive processes behind achieving poetic effects, most pragmaticians employing the lens of Relevance Theory mainly rely on the notion of ‘weak implicature’ (Sperber & Wilson, 1986/1995). However, taking the view held by Sperber and Wilson (2005) that explicatures can too be ‘stronger or weaker’ further, this paper argues that poetic effects on the basis of indeterminacy can similarly be triggered by weak explicatures. Poems by Turkish poet Özdemir Asaf (2020a, 2020b, 2020c) will be employed to provide relevant underpinnings—in terms of reference assignment and enrichment—for our claim.
Explicit performatives in Old Saxon and Old High German
Authors: Concu Valentina 
Published: February 2025

Abstract
This study explores the distribution and frequency of explicit performatives within the Old Saxon and Old High German corpora. Focusing on instances with verbs with meanings such as ‘request,’ ‘command,’ ‘advise,’ and ‘entreat,’ the analysis reveals a partial similarity between the two corpora, particularly in their portrayal of asymmetrical social relationships in religious texts. The findings indicate that higher-ranked addressers, such as God and Jesus, predominantly use verbs of ‘commanding’ and ‘instructing,’ while lower-ranked addressers, mainly believers, employ verbs for ‘requesting’ and ‘begging.’ A comparison with explicit performatives in Old English show a similar use of explicit performatives in these diachronic varieties, likely reflecting common practices in the use of this type of directives. Additionally, the absence of verbs for ‘advising’ or ‘suggesting’ used in explicit performatives aligns with findings in Old English, pointing to a preference for verbs that clearly delineate power dynamics within the texts. This study contributes to our understanding of historical pragmatic variation and the linguistic practices represented in early Germanic texts, offering insights into the portrayal of social hierarchies and power relationships in fictive religious writings.
The Challenge of “Chineseness”: Taiwan’s Identity and Education Policy for Hua-ch’iao and Malaysian Chinese from the 1990s to 2000s
Authors: Hung Tak Wai , Kwan Yuk Sing , Ching Ka Hung 
Published: February 2025

Abstract
This manuscript delves into the complexities of imagined hua-ch’iao identity and its interplay with the Taiwanese education policies for overseas Chinese in Malaysia from the 1990s to the 2000s. By examining the shift in Taiwan’s stance toward Chinese in diaspora, the study underscores the devolution of hua-ch’iao education within the broader context of Taiwan’s quest for a distinct national identity from China. We explore the historical underpinnings and the ideological shifts that informed the policies under the Kuomintang (kmt) and the Democratic Progressive Party (dpp), highlighting the contentious debate over “Chineseness” and its implications for overseas Chinese communities. By tracing the changes in educational policy, the study reflects on the broader questions of identity, nationalism, and the politics of belonging in the era of globalization. It raises critical inquiries about Taiwan’s engagement with overseas Chinese communities and the conceptual challenges of defining “Chineseness” since the twenty-first century.
Chinese Family Businesses, Dialect Groups, and Succession Planning: sme s’ Perseverance Practices in Malaysia
Authors: Lee John Kean Yew , Wong Sau Man 
Published: February 2025

Abstract
Chinese family businesses (cfb s) have been perceived as a cornerstone of Malaysia’s economic and social fabric. The history of cfb s can be referred back to the early fifteenth century, when the Hokkien arrived in Nanyang as the earliest Chinese immigrants to work in trade ports. Since that time, cfb s have undergone an evolution in their structure, from a traditional Chinese form to more professionalized form. To better understand how immigration patterns and traditional skills influence the industries dominated by different dialect groups, the present work adopts a business-history approach to understand small and medium enterprises owned by Chinese families in different dialect groups, namely Besfomec (Hakka), lh Plus (Henghua), and Fung Keong (Cantonese). This study highlights the historical building of business reputations to describe how these Malaysian cfb s in the dialect groups have adapted to Malaysia’s “New Economic Policy.” This study sheds light on the companies’ integration of unique challenges, and the strategies taken by the different dialect groups to achieve market prominence and sustain their businesses over generations. By promoting Nanyang-branded products in Malaysia, this paper aims to demonstrate the importance of Chinese-owned small and medium enterprises’ perseverance practices, the role of the state in fostering public-private partnerships, and inter-ethnic business strengthening during generational change.
Interactive cooking lessons improve attitudes towards food and weight statuses among preschool children
Authors: LeMay-Russell Sarah , Rowell Tiffany , Fernando Nimali , DiEugenio Heidi , Potock Melanie , Chilson Andie , Goldstein Eliza , Jennings Krystal , Zucker Nancy 
Published: February 2025

Objective: Data demonstrate that children in the USA eat below the recommended amount of fruits and vegetables, which has been linked with increased risk of obesity and low weight. Previous school-based interventions promoting healthy eating and enjoyment of food have yielded outcomes such as improved weight and children’s willingness to try novel foods. Therefore, we explored whether interactive cooking lessons could be an effective means of food exposure to fruit and vegetables, and improve weight statuses among young children. Design: This study utilised a community-based intervention. Setting: Head Start classrooms in a southern state of the USA. Methods: Monthly lessons led by classroom teachers introduced students to novel foods and explained nutritional benefits and guiding children through snack preparation and mindful appreciation of the new food. Teachers completed the Food Fussiness and Food Enjoyment subscales of the Child Eating Behaviour Questionnaire and recorded body mass index (BMI) percentiles, which were collected by trained Family Engagement Specialists, for each student at the beginning and end of the school year. Results: Among 614 Head Start students, children demonstrated a significant decrease in food fussiness ( p < .001) and increase in food enjoyment ( p < .001). There were significant changes ( p < .001) in BMI percentile. Children with high weight lost 16.9 percentile points over the year compared to those with healthy weight. Conversely, children with low weight gained 24.7 percentile points. Conclusion: Developmentally appropriate interactive cooking lessons that expose young children to fruit and vegetables in a social setting may be an effective strategy for improving attitudes towards food and weight statuses. Additional data are required to fully understand the mechanisms that cause beneficial weight changes in preschool age children.
Planned approach of virtual training in organizations
Authors: Srivastava Sunaina , Kunte Monica 
Published: February 2025

Background Virtual learning has been widely employed to promote employee learning as technology and globalization have advanced. Critically examining workers’ views on virtual learning is important since their opinions of its design and delivery affect their acceptance of it. Objective The research aims to explore the influence of service quality, information quality (virtual learning training components), and self-efficacy factors on employees’ perceived value and flow at work, towards their learning satisfaction, using perceived organizational support as a moderator in this relationship and employing the SOR architecture as a theoretical foundation. Methods A total of 352 research samples were collected from the IT sector employing convenience sampling and analyzed using a structural equation model. Results The results indicate that service quality, information quality, and self-efficacy positively influenced the employee’s perceived value, flow at work, and learning satisfaction. Employee perceived value and flow at work positively mediated the association between service quality, information quality, self-efficacy, and employee learning satisfaction. Additionally, perceived organizational support had a significant moderating role. Conclusion This research enhances the existing knowledge in the field of information technology and innovative technology adoption by investigating the fast-growing trend of virtual training in the IT industry.
Association of reported sleep disturbances with objectively assessed mild cognitive impairment among adults in the United States
Authors: Shen Chan , Wang Hao , Djiotsop Arthur Nguimatsa , Wiener R. Constance , Pathak Mona , Mitra Sophie , Findley Patricia A , Sambamoorthi Usha 
Published: February 2025

Background: Sleep is a multifaceted phenomenon influenced by both duration and quality. Various sleep disturbances have been associated with mild cognitive impairment, but the role of specific disturbances in mild cognitive impairment pathophysiology remains unclear. This study investigated the associations between distinct sleep disturbances and mild cognitive impairment in adults aged 50 and older using nationally representative data. Methods: Longitudinal data from the Health and Retirement Study were analyzed to explore the association between mild cognitive impairment and three types of sleep disturbances: trouble falling asleep, trouble waking up, and waking up too early. Logistic regression models estimated unadjusted (Model 1) and adjusted associations accounting for sex, race/ethnicity, age, social determinants of health (Model 2), general health (Model 3), depression (Model 4), and pain and physical activity (Model 5). Results: The study cohort included 8877 participants aged ⩾50 years in 2018 (baseline) who were followed up in 2020. Overall, 15.4% reported trouble falling asleep, 23.2% reported trouble waking up, and 12.8% reported waking up too early and being unable to fall back asleep most of the time. Among older adults, approximately 13.1% reported experiencing mild cognitive impairment; The prevalence of mild cognitive impairment was even higher in those who experienced sleep disturbances. The unadjusted odds ratio (uOR) for experiencing trouble falling asleep most of the time was 1.69 (95% CI: 1.42–2.03), for trouble waking up most of the time was 1.31 (95% CI: 1.10–1.57), and for waking up early most of the time was 1.88 (95% CI: 1.51–2.35). However, these positive associations attenuated depending on the covariate adjustment. Conclusions: Nearly one in seven adults had mild cognitive impairment. The relationship between sleep disturbances and mild cognitive impairment has been challenging to delineate. Our findings demonstrate a positive association between sleep disturbances and mild cognitive impairment, although these associations were sensitive to covariate adjustments. These findings suggest multifaceted pathways for reducing the risk of mild cognitive impairment.