bluuebunny/crossref_metadata_2025_split huggingface dataset:
NON BUTANOL EXTRACTABLE IODINE IN THE SERUM OF EUMETABOLIC ADULT GOITRE PATIENTS
Authors: Lamberg B.-A. , Hintze G. , Karlsson R. 
Published: December 2008

ABSTRACT
In studies on endemic goitre there were 20 patients who had more than a normal proportion of butanol insoluble iodine in the serum. All these patients were eumetabolic adults with a goitre ranging in size from 50 to 300 g. The insoluble portion of the PB131I (NBE131I) varied from 25 to 80 per cent, the PB131I from 0.005 to 0.94% per litre. Radiochromatography of the extract revealed that thyroxine made up 30 to 100 per cent, the remainder being iodide. In one case instead of thyroxine, 48 per cent of triiodothyronine was found. In one case traces of diiodotyrosine were found 6 hours after the test dose.
Enzymatic hydrolysis increased the extractability in two cases but failed to do so in two other cases.
The responses of PBI, PB131I to stimulation with thyrotrophic hormone were studied in some instances. There was usually a good response of PBI and PB131I. The reaction of NBE131I, however, was nil, or very small. The ratio between the values obtained after and before stimulation varied from 1.0 to 1.3. In contrast there was a marked increase in the difference PB131I – NBE131I which presumably represented thyroid hormones. The ratio varied from 2.5 to 3.2. In one case restudied 5 weeks and 3 months after subtotal thyroidectomy the same situation was observed.
The occurrence of NBE131I was observed in two members of a family in two instances. In some other families the pedigree was also highly suggestive of an inherited disorder, although it must be admitted that the
STUDIES ON THE HORMONAL CONTROL OF THE MILK-EJECTION ACTIVITY IN LACTATING RABBITS
Authors: Kullander Stig 
Published: December 2008

ABSTRACT
A study was made of the effect of synthetic oxytocin (Syntocinon Sandoz) and synthetic lysine-vasopressin (Vasopressin Sandoz) on the milk-ejecting activity of the lactating mammary gland of the rabbit. Both drugs were given by intravenous infusion except in some cases where oxytocin was injected intravenously in single doses.
Depending on the sensitivity of the mammary gland and the amount of oxytocin administered, infusion of this hormone gave a prolonged tonic increase in the milk pressure or a rhythmic series of pressure increases. Both these reactions could be counteracted appreciably by giving vasopressin simultaneously in doses of the order of 1:4 to 1:1 in relation to the dose of oxytocin. After withdrawal of vasopressin the original effect of the oxytocin in the milk-gland pressure was slowly resumed. Doses of vasopressin with ratios of 1:1000 – 1:5 or saline had little or no effect at all.
Intravenous administration of adrenaline also caused transient suppression of the effect of the oxytocin infusion. There was no evidence to indicate that vasopressin potentiated this effect.
These findings are discussed in relation to the clinical findings of disturbance in the milk let-down during different stress situations. Both vasopressin and adrenaline might be released during stress.
EXPERIMENTAL STUDIES ON THE EFFECTS OF OVARIECTOMY AND HYPOPHYSECTOMY ON BLASTOCYST IMPLANTATION IN THE RAT
Authors: Zeilmaker G. H. 
Published: December 2008

ABSTRACT
The effects of ovariectomy and hypophysectomy on the third and fourth day of pregnancy on blastocyst implantation have been studied. The day on which vaginal sperm was found was considered as day 0 of pregnancy. It was found that ovariectomy at the 1st h on day 4 of pregnancy did not interfere with implantation when progesterone substitution was given. The same operation performed at the 17th h on the third day almost completely prevented implantation. Hypophysectomy followed by luteotrophic hormone treatment at the 17th h on day 3, however, did not interfere with implantation whereas pituitary extirpation on the 13th h on day 3 prevented implantation in most cases.
A single administration of nembutal, atropine and reserpine several hours prior to the time hypophysectomy would have interfered with implantation did not delay implantation by 24 hours.
Some cases of incomplete implantation are described in which implantation sites of normal size occurred simultaneously with unimplanted blastocysts in the same uterus. These blastocysts implanted following the administration of oestradiol.
The results are discussed in relation to current views on the hormonal regulation of blastocyst implantation.
A SENSITIVE ANTI-ANDROGEN ASSAY: ANTAGONISM OF LOCALLY APPLIED ANDROGEN BY A-NORPROGESTERONE INUNCTED ON THE CHICK COMB
Authors: Lerner Leonard J. , Bianchi Albert , Dzelzkalns Margaret 
Published: December 2008

ABSTRACT
A completely local and highly sensitive anti-androgen assay employing the chick comb has been described.
Testosterone dissolved in oil was applied in daily volumes of 0.005 ml to one side of the comb by means of microsyringe. The anti-androgen, A-norprogesterone, in a similar volume of sesame oil was inuncted daily on the other side of the comb. After 7 days of treatment, the chick comb weights increased directly with the quantity of androgen applied and were inhibited from responding to the androgen in a direct relationship to the dose of A-norprogesterone.
DIURNAL RHYTHM IN THE ADRENAL CORTICAL SECRETION AND IN THE RATE OF METABOLISM OF CORTICOSTERONE IN THE RAT
Authors: Saba G. C. , Saba P. , Carnicelli A. , Marescotti V. 
Published: December 2008

ABSTRACT
Plasma levels and adrenal production of corticosterone (11β,21-dihydroxy-pregn-4-ene-3,20-dione) have been determined at different times of the day in male rats. Diurnal variations both in steroid blood levels and in adrenal secretion have been found. An attempt was made to study the rate of disappearance of the hormone in the morning, afternoon, evening, and at night.
DIURNAL RHYTHM IN THE ADRENAL CORTICAL SECRETION AND IN THE RATE OF METABOLISM OF CORTICOSTERONE IN THE RAT
Authors: Saba G. C. , Carnicelli A. , Saba P. , Marescotti V. 
Published: December 2008

ABSTRACT
An absence of diurnal rhythm, and a reduction of adrenal secretion, plasma levels and rate of disappearance of corticosterone (11β,21-dihydroxy-pregn-4-ene-3,20-dione) are observed in the rat after electrolytic lesions of the eminentia mediana.
The possibility of a direct control of the hormonal metabolism by the hypothalamus is discussed.
ON THE VALUE OF SOME PARAMETERS OF ADRENOCORTICAL GLUCOCORTICOID FUNCTION
Authors: Van Der Straeten M. , Vermeulen A. , Orie N. , Regniers P. 
Published: December 2008

ABSTRACT
The authors studied the correlation between cortisol production, as measured by an isotope dilution method, and the urinary excretion of total and free Porter-Silber chromogens, as well as of 17-ketogenic steroids. Although a significant correlation exists between total Porter-Silber chromogens, 17-ketogenic steroid excretion and cortisol production, discrepancies are occasionally observed. Hence, different colorimetric methods should be used to assess the glucocorticoid activity of the adrenal cortex.
HISTOLOGICAL CHANGES IN EXPERIMENTAL EXOPHTHALMOS OF CARASSIUS AURATUS
Authors: Brunish Robert , Sørensen Bente 
Published: December 2008

ABSTRACT
Carassius auratus were injected with pituitary exophthalmos-producing substance and the subsequent histological changes studied. Oedema, infiltration of macrophages in the retrobulbar area, muscle swelling and splitting, and increase of mucinous substance were noted. Since such changes have been reported for exophthalmic human patients and guinea pigs, it is suggested that the proptosis induced in fish has a genesis similar to that of the other species. Mast cells were hardly observed in Carassius auratus whenever the histological sections were exposed to aqueous solutions. With due precautions, however, an increase in mast cell number in the intestinal submucosa was noted.
METABOLIC EFFECTS OF SHORT TERM ADMINISTRATION OF HUMAN GROWTH HORMONE IN INFANCY AND EARLY CHILDHOOD
Authors: Vest M. , Girard J. , Bühler U. 
Published: December 2008

ABSTRACT
The metabolic effects of short term administration of human growth hormone were studied in four infants two weeks to 2½ months old and three children 11 months to 3¼ years of age. They responded qualitatively in the same way as older children and hypopituitary dwarfs. Quantitatively the infants responded to a lesser extent than older subjects. The most constant findings are in order of decreasing response: Fall in urinary creatine, urea nitrogen and total nitrogen excretion and decreasing blood concentration of urea and residual nitrogen. An increase in α-amino-nitrogen excretion is also a fairly common finding. There is only a slight rise in the fasting level of free fatty acids in the serum in response to HGH injection in the infants. In the children this effect is already more pronounced.
A fall in urinary sodium, potassium and phosphorus does not occur as regularly as in older subjects. An increase in ketone bodies in the urine was observed in some infants and children.
The possible mechanisms and significance of the diminished response of infants to exogenous growth hormone are discussed.
THE »EARLY-ANDROGEN« SYNDROME; ITS DEVELOPMENT AND THE RESPONSE TO HEMI-SPAYING
Authors: Swanson H. E. , van der Werff ten Bosch J. J. 
Published: December 2008

ABSTRACT
The »early-androgen« syndrome in the rat – i. e. anovulatory ovaries in adult females after a single injection of testosterone propionate (TP) within a week of birth – may not become apparent until some time after the attainment of sexual maturity. Large doses (50 or 100 μg) of TP were effective earlier than lower doses (5 or 10 μg). Rats which received 5 μg TP were ovulating at 10 weeks of age, mated but were infertile at 13 weeks of age, and were anovulatory at 21 weeks. In rats between 10 and 13 weeks old there was a marked fall in the number of corpora lutea in the ovaries of animals which had been given 5 μg TP.
Hemi-spaying was followed by compensatory growth of the remaining ovary which consisted of corpora lutea in ovulating, and of follicles in anovulatory rats; little or no compensatory weight increase occurred in animals which seemed to be in the transition stage from the ovulatory to the anovulatory condition.
ÜBER DEN EXTREMEN GESCHLECHTS-DIMORPHISMUS IM WACHSTUM DER MOSCHUSENTE, CAIRINA MOSCHATA FLEMM.
Authors: von Faber Hans 
Published: December 2008

ABSTRACT
The growth of the long bones and the development of the epiphyseal cartilage were studied in 50 male and 33 female Muscovy ducks from the time of hatching up to the 18th week. An average body weight was measured of 1990 ± 18 g in the females and of 2838 ± 99 g in the males after 10 weeks. No further increase in the body weight was noted in the females. The males continued to grow up to the 18th week and reached a final weight of 4101 ± 62 g. In the femur, the tibiotarsus, and the tarsusmetatarsus a sex difference occurred in the 2nd week and became significant after 4 weeks. The greatest difference was observed in the tibiotarsus which reached a length of 125.4 ± 0.7 mm in the male and 103.2 ± 1.1 mm in the female after 10 weeks. In both sexes the greatest increase of bone length was noted within the first 4 weeks. The bone growth ceased and the epiphyseal cartilage disappeared with about 8 weeks in the female and with about 10 weeks in the male. The humerus, the ulna and the radius were somewhat larger in the female than in the male after 4 weeks. Thereafter a more rapid growth was seen in the male. The greatest sex difference was observed in the humerus which measured 136.0 ± 1.6 mm in the male and 113.8 ± 0.9 mm in the female after 10 weeks. In contrast to the leg bones the greatest increase in body length occurred between the 6th and the 8th week. The bone growth ceased after 10 weeks in the female, and after 12 weeks in the male. Growing males which were treated with 35 mg stilboestrol weighed 2034 ± 90 g after 10 weeks. The long bones only grew to 85–90 % of the normal length. Birds which were allowed to survive a further 8 weeks after the treatment, attained a body weight of 3494 ± 111 g without any further increase in the length of the leg bones and only a slight increase in the length of the wing bones. The dwarfing effect of stilboestrol was not caused by a precocious disappearance of the epiphyseal cartilage. An identical treatment with stilboestrol had in Pekin ducks no effect on body weight or on the long bones.
PROTEINS OF NORMAL AND PATHOLOGICAL HUMAN THYROID TISSUE
Authors: Stanley P. G. 
Published: December 2008

ABSTRACT
Salting-out curves, analytical ultracentrifugation and paper electrophoresis were used to examine saline extracts of 21 human thyroid glands or portions of glands, both normal and pathological. A simplified procedure of plotting salting-out curves was used. The glands could be divided into 2 groups, the first characterized by a high proportion of protein sedimenting with S20,w 18–20 (55–75 %) and a low proportion of protein sedimenting with S20,w 4 (20–40%), the second characterized by a low proportion of protein sedimenting with S20,w 18–20 (20–55%) and a high proportion of protein sedimenting with S20,w 4 (45–75%). Group 1 comprises normal glands, multinodular goitres, a follicular-alveolar carcinoma and an anaplastic carcinoma containing much follicular tissue; group 2 comprises a follicular and trabecular adenoma, a follicularpapillary carcinoma, anaplastic carcinomata and toxic goitres. Protein sedimenting with S20,w 18–20 could be correlated with protein salting out between 35 and 45% saturation with ammonium sulphate (21 points, correlation coefficient 0.931, P < 0.001 by t test) and there was some correlation between protein sedimenting with S20,w 4 and protein salting out between 20 and 30 % saturation with ammonium sulphate provided some points were omitted (18 points, correlation coefficient 0.600, P < 0.01 by t test). It is suggested that the protein salting out between 20 and 30 % saturation is a nucleoprotein.
EFFECT OF SOME THYROXINE ANALOGUES ON HYPOTHYROID TADPOLES
Authors: Henriques U. 
Published: December 2008

ABSTRACT
Using a new tadpole assay for thyroid hormone which has previously been described, the activities of some thyroxine analogues have been investigated. Apart from the usual immersion technique, microinjection was also used for administration of drugs.
Diiodotyrosine was found to have no effect. Diiodothyronine only had a small effect. This compound as well as triprop, triac and tetrac gave dose response curves quite unlike those of triiodothyronine and thyroxine, so that an estimation of the relative activity could hardly be made. The implications of this finding is discussed.
D-thyroxine was found to be 7 times less active than L-thyroxine.
Triiodothyronine which in previous immersion experiments was found to be about 9 times as active as thyroxine was in injection experiments about 5 times as active as thyroxine.
None of the fatty acid derivatives investigated was more active than thyroxine if given by injection. Contamination with small amounts of these derivatives in the test material therefore does not invalidate the results obtained by the present method.
THE EFFECT OF THYROTROPHIC HORMONE ON THE POTASSIUM CONTENT OF MUSCLE AND LIVER TISSUE AND ON THE BLOOD SUGAR LEVEL IN GUINEA PIGS
Authors: Gedda Per Olof 
Published: December 2008

ABSTRACT
The investigation showed that a single subcutaneous injection of thyrotrophic hormone (TSH) in young guinea pigs does not produce any change in the potassium content of striated muscle or of the liver within 2 hours of the injection, but does induce hyperglycaemia within this period.
In a previous investigation (Gedda 1960), it was demonstrated that an equally large dose of TSH produced a marked increase in the potassium content of the thyroid gland of the guinea pig within 2 hours. The observations made in the present investigation support the assumption that the increase in potassium in the thyroid gland is specific to the gland after stimulation with TSH and that the increase is not secondary to a primary hypoglycaemia.
DIABETES INSIPIDUS ASSOCIATED WITH EXOPHTHALMIC GOITER
Authors: del Castillo Enrique B. , Trucco Eduardo , Radzyminski Delia 
Published: December 2008

ABSTRACT
The case of a 42 year old female with diabetes insipidus, exophthalmic goiter, lipodystrophy and amenorrhoea is described. The association of diabetes insipidus and hyperthyroidism is not frequently observed and only a few cases have been reported in the literature.
Attention is drawn to what happened to this patient during the Carter-Robbins test and to her death at the end of it.
CORTISOL METABOLISM IN THE NEWBORN
Authors: Aarskog D. , Stoa K. F. , Thorsen T. 
Published: December 2008

ABSTRACT
Following intravenous administration of cortisol-4-14C to seven full-term and five premature newborn infants, the urinary excretion of free and conjugated substances was measured in different fractions. Three normal adult subjects served as controls.
The study comprised the following fractions: a) Free »lowpolar« fraction, extractable with methylene dichloride. b) Free »high-polar« fraction, extractable with ethyl acetate. c) »Low-polar« glucuronoside fraction, extractable with methylene dichloride following β-glucuronidase hydrolysis. d) »High-polar« glucuronoside fraction, extractable with ethyl acetate following β-glucuronidase hydrolysis. e) Solvolysis fraction, extractable with ethyl acetate after previous removal of the free and glucuronoside conjugated fractions and subsequent solvolysis.
The excretion of total radioactivity in the group of normal newborns was found to be delayed as compared with the adult subjects. The unconjugated fractions were found to be somewhat higher in the newborn than in the adult subjects. On the other hand, the »low-polar« glucuronosides were essentially lower. These deviations from the findings in normal adults were still more pronounced in the group of premature newborns. An inverse relationship was demonstrated between the percentage excreted as glucoronosides and the maximum serum bilirubin level in the neonatal period.
PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE
Authors: Donini P. , Puzzuoli D. , Montezemolo R. 
Published: December 2008

ABSTRACT
Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C).
The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity.
The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2 and β globulins.
PURIFICATION OF GONADOTROPHIN FROM MENOPAUSAL URINE BY GEL FILTRATION ON SEPHADEX
Authors: Donini P. , Puzzuoli D. , D'Alessio I. 
Published: December 2008

ABSTRACT
The further purification of HMG obtained from postmenopausal urine with kaolin-acetone, DEAE-C and permutit chromatography was performed using gel filtration on Sephadex (G 200).
The potency of the purest fraction isolated was about 2.5 times higher than that of the starting material.
The total recovery of biological activity was 96 %.
THE INFLUENCE OF ANTIDIURETIC HORMONE ON PANCREATIC EXOCRINE SECRETION
Authors: Perks A. M. , Schapiro H. , Woodward E. R. 
Published: December 2008

ABSTRACT
Intravenous injections of an ADH preparation into unaesthetized dogs resulted in reduced rates of pancreatic exocrine flow, provided that the doses were above 4.2 mU/kg dog. There was a direct relationship between the percentage inhibition of pancreatic flow and the log of the dose of ADH administered. Oxytocin had no such effect in doses up to 83 mU/kg dog. During ADH inhibition, sodium, potassium and bicarbonate concentrations in the juice remained unchanged but protein and amylase concentrations increased.
Sodium thioglycollate, sodium hydroxide, and ultraviolet light, all of which destroy ADH, destroyed the pancreas-inhibiting factor. Intravenous injection of hypertonic sodium chloride and hypertonic glucose solutions also reduced the rate of pancreatic exocrine flow.
It is suggested that ADH can cause the isosmotic reabsorption of electrolytes from pancreatic secretion by an action on the cells of the pancreatic ducts.
HORMONE ASSAYS DURING RECURRENT EXCESSIVE HAIRGROWTH IN PREGNANCY
Authors: Turunen Aarno , Pesonen Sykkö , Zilliacus Harry 
Published: December 2008

ABSTRACT
Three cases of recurrent excessive hairgrowth during pregnancy are described. The hormone excretion of two of these women was studied before and after stimulation tests. The patients received 40 IU of corticotrophin as an intravenous infusion and in addition a total of 3 mg fluorocortisone to produce suppression of the adrenals. Urinary steroids were determined before and during the stimulation tests. Four patients who were in the first trimester of pregnancy served as controls.
After the legal termination of pregnancy incubation experiments with placental tissue were carried out to determine the ability of the placenta to convert the 17-OH-group of testosterone to the 17-keto-group. The results of these experiments were compared with those in normal subjects. In one case pregnancy had been legally terminated, and in the other the placenta was full-term.
In both patients an increased 17-OH-progesterone secretion and some block in steroid synthesis were responsible for dysfunction. The excretion of total 17-ketosteroids, epiandrosterone and androsterone were higher in these patients than in the control group.
In incubation experiments of placental tissue it was confirmed that the 17β-dehydrogenase activity of the patients was lower than in the control placenta. Hence, the inactivating capacity of testosterone was less than that of the control group. This phenomenon may be responsible for an increased level of testosterone in the plasma of the patients and of excessive hairgrowth during pregnancy.
Of the six children born to the three mothers with recurrent excessive hairgrowth during pregnancy, three had severe congenital malformations, in one case of the heart and in two cases of the extremities.
QUANTITATIVE STUDIES ON ISOLATED PANCREATIC ISLETS OF MAMMALS
Authors: Hellerström Claes , Täljedal Inge-Bert , Hellman Bo 
Published: December 2008

ABSTRACT
Quantitative studies of non-specific acid phosphatases were performed on isolated pancreatic islets from obese-hyperglycaemic mice. The islets of these animals are composed of a rather pure population of B cells. The following observations were made:
Acid phosphatases originating in the islet tissue, showed maximal enzyme activities at about pH 3.5 and 5.3 using p-nitrophenyl phosphate as substrate. The acid phosphatase activity of the exocrine tissue showed a single distinct maximum at about pH 5.3. The islet acid phosphatases were inhibited by sodium fluoride, sodium tartrate and formaldehyde. They were stable against storage in crude tissue homogenates at + 4° C and + 20° C for 48 hours. The pancreatic islets exhibited a significantly higher acid phosphatase activity than the exocrine parenchyma. Starvation for 7 days did not alter the enzyme levels in the islets or acini when measured at pH 5.3, while a probably increased enzyme activity was obtained in both these regions at pH 3.5. There was no evidence for a relationship between the insulin secretion and the acid phosphatase activity of the B cells.