bluuebunny/crossref_metadata_2025_split huggingface dataset:
Retrospective c‐erbB‐2 immunostaining in aspiration cytology of breast cancer
Authors: Jorda M. , Ganjei P. , Nadji M. 
Published: February 2007

AbstractFifty fine‐needle aspiration cytologies of breast that were diagnosed as carcinomas were retrieved from the files and retrospectively evaluated for the expression of c‐erbB‐2 oncoprotein using standard immunocytochemical methods. Corresponding histologic sections of all tumors were similarly studied.Seventeen fine‐needle aspirates (34%) reacted positively for the presence of c‐erbB‐2 oncoprotein. All but one (32%) of the corresponding tissue sections were also positive for c‐erbB‐2 by immunohistochemistry. All positive cases were infiltrative ductal carcinomas with a preponderance of the comedo type. Positive reactions were localized in the cytoplasmic membrane of tumor cells. The staining was either present in all cells throughout a tumor, or it was completely absent. We conclude that immunocytochemistry for c‐erbB‐2 oncoprotein can be performed on fine‐needle aspiration cytology samples that are previously fixed and stained with the Papanicolaou technique. Furthermore, the sensitivity of immunostaining results are comparable to that obtained in histologic sections. Diagn Cytopathol 1994; 11:262–265. © 1994 Wiley‐Liss, Inc.
Small‐cell carcinoma of the ovary in peritoneal fluid
Authors: Selvaggi Suzanne M. 
Published: February 2007

AbstractTwo cases of small‐cell carcinoma of the ovary in the ascitic fluid and peritoneal/pelvic washings of a 30‐ and 28‐yr‐old woman, respectively, are presented and discussed. Smear preparations from the ascitic fluid showed loose clusters and single malignant cells with scant cytoplasm and nuclei with smooth to irregular nuclear membranes, granular chromatin, and small nucleoli. In the second case peritoneal/pelvic washing specimens contained clusters and single malignant cells with a moderate amount of cytoplasm and nuclei with smooth nuclear membranes, granular, clumped chromatin, and prominent nucleoli. Histology confirmed the diagnosis of small‐cell carcinoma of the ovary. These are the first reported cases of this rare ovarian neoplasm present on fluid cytology. Its differentiation from other small‐cell neoplasms on peritoneal fluid cytology from young women is discussed. Diagn Cytopathol 1994; 11:266–270. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of salivary gland mycoses
Authors: Raab Stephen S. , Thomas Patricia A. , Cohen Michael B. 
Published: February 2007

AbstractThis report details the fine‐needle aspiration biopsy (FNAB) cytomorphologic features of two cases of salivary gland mycosis. Both patients had acquired immunodeficiency syndrome (AIDS) and presented with parotid gland masses. The first patient had Histoplasmosis with secondary infection by Candida. Cytopathologically, the FNAB smears showed classic features of a deep‐seated mycosis characterized by necrosis and scattered fungal forms. The second patient had a colonizing sialadenitis caused by either Asperigillus or Fusarium. Cytopathologically, the findings were similar to those seen in aspergillomas of the lung orparanasal sinuses with numerous hyphal forms and an absence of an inflammatory response. Because mycotic disease can induce a wide spectrum of pathogenic change, other benign or malignant, solid or cystic lesions enter into the differential diagnosis. Diagn Cytopathol 1994; 11:286–290. © 1994 Wiley‐Liss, Inc.
Melanocytic schwannoma: The cytologic aspect in fine‐needle aspiration cytology (FNAC): Report of a case located in the spinal cord
Authors: Sola‐Pérez J. , Pérez‐Guillermo M. , Bas‐Bernal A. , Giménez‐Bascuñana A. , Montes‐Clavero C. 
Published: February 2007

AbstractWe describe a case of intramedullary melanocytic schwannoma (MS) studied by means of fine‐needle aspiration cytology (FNAC).The main cytologic features were (1) large three‐dimensional clusters overshadowed by heavy pigment deposits which tested positive for Fontana Masson (and bleached with potassium permanganate), HMB‐45, Vimentin, and S‐100 protein; (2) loose syncytial monolayered sheets with very little pigment deposit; and (3) isolated spindle cells with polarized nucleus and tapered, thin cytoplasmic ends.Both the cytologic features and the immunophenotypic profile were nonspecific and on their own were insufficient to allow the diagnosis of MS to be reached. Clinical data and the magnetic resonance image (MRI) in conjuction with the cytologic findings did suggest the diagnosis of intramedullary MS. Diagn Cytopathol 1994;11:291–296. © 1994 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of recurrent and metastatic mixed mesodermal tumors
Authors: Mourad Walid A. , Sneige Nour , Katz Ruth L. , Caraway Nancy P. , Fanning Tina V. 
Published: February 2007

AbstractWe report the cytological and clinical findings of 16 fine‐needle aspirates (FNAs) performed on recurrent (n = 6) and metastatic (n = 10) mixed mesodermal tumors (MMMTs). The median interval between the primary diagnosis and FNA was 16 mo. Primary sites were the endometrium (n = 11), the ovary (n = 3), the cervix (n = 1), and pelvic soft tissue (n = 1). Primary tumors showed carcinoma with homologous mesenchymal components in 13 cases and focal heterologous elements in three (two chondrosarcomas and one rhabdomyosarcoma). The FNAs showed carcinoma in all 16 cases, with adenocarcinoma differentiation in three, Mesenchymal elements were identified in aspirates of three recurrent and two metastatic lesions. They were all homologous. No heterologous mesenchymal elements were identified in the aspirates. We conclude that mesenchymal components in FNAs of MMMTs are less likely to be seen in metastatic lesions, and that heterologous mesenchymal components are rarely seen in these aspirates even in recurrent disease. These findings confirm that the epithelial component is responsible for the malignant behavior of MMMTs, and suggest that these lesions may need to be classified as sarcomatoid carcinomas rather than true carcinosarcomas. Diagn Cytopathol 1994;11:328–332. © 1994 Wiley‐Liss, Inc.
Comparative studies of the same adenocarcinoma cells, macrophages, and mesothelial cells by light microscopy, scanning electron microscopy, and transmission electron microscopy
Authors: Kaneko Chiyuki , Niimi Hajime , Shinzato Masanori , Shamoto Mikihiro 
Published: February 2007

AbstractThe identification of cells in body cavities of cancer patients is sometimes difficult to make. In order to make a definite cytological diagnosis, we observed the same cells by using light microscopy (LM)‐scanning electron microscopy (SEM)‐transmission electron microscopy (TEM). In this study we first stained cells by the Papanicolaou method after fixation in 1% glutaraldehyde for LM, and then attempted to observe them successively by SEM‐TEM after fixation in 1% paraformaldehyde and 1.25% Os04. Our method and procedures in examining successively one and the same cells in body cavity fluids by using LM, SEM, and TEM ensured accurate discrimination among adenocarcinoma cells, mesothelial cells, and macrophages. The results of this study suggest that LM‐SEM‐TEM may be of diagnostic value in distinguishing among mesothelial cells, macrophages, and adenocarcinoma cells. This method also succeeded in disclosing differences between the ultrastructure of the cell surfaces, and those of the cytoplasm, and of the nuclei It is desirable that LM‐SEM‐TEM observation can be introduced into various aspects in order to obtain an improvement in the diagnosis by cytologic examination, the judgment of therapeutical effects, drug selection, and prognostic presumption. Diagn Cytopathol 1994; 11:333–342. © 1994 Wiley‐Liss, Inc.
Interobserver variability of cervical smears with squamous‐cell abnormalities: A philadelphia study
Authors: Young Nancy A. , Naryshkin Sonya , Atkinson Barbara F. , Ehya Hormoz , Gupta Prabodh K. , Kline Tilde S. , Luff Ronald D. 
Published: February 2007

AbstractThe reproducibility of reporting squamous lesions by the Bethesda System (TBS) was evaluated by distributing 20 slides to be classified among 5 panelists considered experts in the field of cytopathology. Four cases were chosen for their classic morphology and the remainder were foreseen to produce possible discrepancies within one diagnostic category. For 7/20 (35%) cases there was unanimous agreement. Participants disagreed within one category of magnitude for seven (35%) cases. In six (30%) cases there was a range of more than one category disagreement. However, additional written comments modifying TBS diagnoses often diminished the clinical significance of these discrepancies. We conclude that despite the important role of TBS in standardization of Pap smear reports, a great degree of subjectivity exists in classifying squamous abnormalities without “classic” morphology. The lack of reproducibility should be taken into account in cytology proficiency testing. Diagn Cytopathol 1994;11:352–357. © 1994 Wiley‐Liss, Inc.
Utility of polyclonal and monoclonal antibodies against carcinoembryonic antigen in hepatic fine‐needle aspirates
Authors: Rishi Mazhar , Kovatich Albert , Ehya Hormoz , Davila Rosa M. 
Published: February 2007

AbstractTo assess the usefulness of polyclonal and monoclonal antibodies against carcinoembryonic antigen (CEA) in the differential diagnosis of hepatocellular carcinoma (HCC) vs. metastatic adenocarcinoma (MA), we studied 25 cases of fine‐needle aspirates (FNA) of hepatic lesions. The material consisted of 9 primary HCCs, 8 MAs, and 8 benign hepatic aspirates. For immunostaining, the avidin‐biotin complex technique was performed on paraffin sections of cell blocks, using a standardized automatic immunostainer. Specific bile canalicular immunostaining with polyclonal CEA (pCEA) antibody was present in five of eight (5/8) benign hepatic aspirates and eight of nine (8/9) HCCs. Diffuse cytoplasmic immunostaining with pCEA antibody was present in four of eight (4/8) MAs. None of the aspirates showed any positive immunostaining with monoclonal CEA (mCEA) antibody. We conclude that: (1) pCEA antibody is useful in the evaluation of hepatic FNAs. Diffuse cytoplasmic staining is seen in MAs, whereas canalicular immunostaining pattern is an indication of benign or malignant hepatocytes. (2) Paraffin‐embedded cell blocks made from hepatic aspirate material are suitable for immunostaining with polyclonal CEA antibody. (3) mCEA antibody has no value in the diagnosis of HCC. Diagn Cytopathol 1994; 11:358–362. © 1994 Wiley‐Liss, Inc.
Nocardiosis diagnosed by lung FNA: A case report
Authors: Busmanis Inny , Harney Mary , Hellyar Andrew 
Published: February 2007

AbstractA 63‐yr‐old woman with systemic lupus erythematosus (SLE) diagnosed 12 mo previously and treated with prednisolone and cyclophosphamide presented with recent fever and dyspnoea. the etiology of a 3 cm diameter centrally cystic coin lesion in the lower lobe of the left lung was obscure. Blood cultures and sputum examination had been non‐contributory, and the diagnosis of Nocardia asteroidesinfection was initially made by cytologic examination of material obtained by lung fine‐needle aspiration (FNA). It is notoriously difficult to detect this organism by conventional sputum examination or with histologic sections, and it has rarely been detected by lung FNA. If this organism is demonstrated, appropriate microbiologic cultures for confirmation and susceptibility testing should be instituted. Long‐term antimicrobial therapy is needed. in this case, complete resolution of the lung lesion followed 5 mo of therapy.
Diagnosis of metastatic appendiceal adenocarcinoid in liver by fine‐needle aspiration cytology
Authors: Argani Pedram , Van Hoeven Karen H. , Artymyshyn Renee L. 
Published: February 2007

AbstractA 70‐yr‐old woman presented with symptoms clinically attributed to acute appendicitis. An adenocarcinoid tumor of the appendix was resected, followed by right hemicolectomy with negative lymph nodes. Seven years later, a fine‐needle aspiration biopsy was performed on one of multiple hepatic nodules, revealing metastatic adenocarcinoid. the cytopathologic features of the metastatic tumor are described.
Calcium oxalate crystals in benign breast cyst fluid
Authors: Mihaescu A. , Burri G. 
Published: February 2007

AbstractWe have recently observed a strongly birefringent material of varying shapes and sizes in a benign breast cyst fluid specimen from a 52‐yr‐old woman with suspicious mammographic microcalcifications. the finding of calcium oxalate crystals in the breast cyst fluid should be regarded as a recognition of a particular type of calcification, easily overlooked with the conventional light microscopy. to date, we are unaware of any cytologic reports of this type of calcification in the breast.
Calcifying epithelial odontogenic tumor (Pindborg's tumor) on fine‐needle aspiration biopsy smears: A case report
Authors: Fulciniti Franco , Vetrani Antonio , Zeppa Pio , Califano Luigi , Palombini Lucio 
Published: February 2007

AbstractA 44‐yr‐old woman with a long‐standing history of painful mandibular swelling was hospitalized for the diagnostic evaluation of an osteolytic mandibular lesion in the premolar‐canine area. Fineneedle aspiration biopsy (FNAB) was performed and cytologic smears were characterized by clusters, sheets, and rare isolated pleomorphic cells of the squamoid type, blocks of amorphous material encircled by fibroblasts, and occasional calcifications. A cytological diagnosis of Pindborg's tumor was made, which was confirmed by histopathological examination. Evaluation of DNA ploidy by semiautomated image cytometry produced an aneuploid histogram. the patient is free of residual neoplasm at a 12‐mo post‐operative interval. the cytological differential diagnosis of this rare odontogenic neoplasm is discussed briefly.
Transtracheal needle aspiration
Authors: Roche David H. , Wilsher Margaret L. , Marion Gurley A. 
Published: February 2007

AbstractA series of 27 transtracheal needle aspiration biopsies performed on 25 patients were reviewed. A rigid bronchoscope and rigid needle were used. the biopsies were performed as staging procedures in patients with bronchogenic carcinoma, or to obtain diagnoses in patients with mediastinal adenopathy. A cytopathologist attended 11 biopsies and microscopically examined a direct smear to determine adequacy. Repeat biopsies were taken at the time if necessary. A diagnosis was obtained in 9/11 (81%) of these cases. Only 3/16 biopsies (18.8%) performed in the absence of a pathologist were diagnostic. All negative cases were subsequently found to have disease (2 sarcoidosis, 13 cancer) by other methods including mediastinoscopy in 5 cases. in the patient group sampled, the technique of rigid needle and rigid bronchoscope with a cytopathologist in attendance resulted in a much higher sensitivity for the test than has been previously reported. © 1995 Wiley‐ Liss, Inc.
Benign spindle and inflammatory lesions of the breast: Diagnosis by fine‐needle aspiration
Authors: Bardales Ricardo H. , Stanley Michael W. 
Published: February 2007

AbstractWe describe fine‐needle aspiration (FNA) findings in 7 patients with breast masses showing numerous spindle cells in smear material. the benign nature of these lesions was confirmed by clinical follow‐up (n = 4) or surgical excision with histologic examination (n = 3). the final diagnosis included one case each of myofibroblastoma, juvenile hypertrophy, post‐FNA reactive stromal proliferation, stromal induction in proliferative fibrocystic disease, and subareolar abscess. There were two instances of granulation tissue. the case of stromal induction was initially felt to be suspicious for phyllodes tumor. the post‐FNA stromal reaction was considered sufficiently atypical to warrant histologic investigation. the importance of historical and physical examination findings in accurate interpretation of these uncommon cases is emphasized. Some will require histologic assessment for final diagnosis. © 1995 Wiley‐ Liss, Inc.
High grade squamous cervical lesions following negative papanicolaou smears: False‐negative cervical cytology or rapid progression
Authors: Hatem Fadi , Wilbur David C. 
Published: February 2007

AbstractThe cervical cytologic finding of a high grade squamous intraepithelial lesion (HGSIL) or carcinoma shortly following a negative smear is unusual. to determine if such cases are due to screening errors, inadequate sampling, or rapidly progressive lesions, a review of negative smears (immediately preceding a diagnosis of HGSIL or carcinoma by at most 2 yr) was performed on 17 patients (all such patients in our 1990–92 files, representing 6% (17/290) of such diagnoses). the time between negative smear and positive result averaged 9.3 mo. Eighty‐eight percent of these patients had prior histories of a cytologic abnormality.Upon review, 16 of 17 “negative” smears contained a cytologic abnormality. Atypical immature squamous metaplastic type cells (AISMTs) were found in 11 of 16 cases, atypical squamous cells or slight dysplasia were found in 5 of 16, HGSIL was found in 5 of 16, and atypical cells of undetermined type and origin were noted in 1 of 16. Combinations of the above diagnoses were present in 6 of 16 cases. the number of abnormal cells per slide was estimated, with < 10 cells in 1 case, <50 in 4 cases, 50–100 in 7 cases, 100–200 in 3 cases, and > 200 in 1 case.In this study, screening and/or evaluation errors were the dominant cause of false‐negative cytologic diagnoses, with AISMTs accounting for the most commonly missed or misinterpreted cells. These cells are small and have hyperchromatic nuclei with elevated nucleus to cytoplasmic ratio and nuclear irregularities. They may occur singly or in small groups, and may be interpreted as small histiocytes or missed entirely on screening. A careful search for such cells is warranted, and in their presence, colposcopy and biopsy should be undertaken. © 1995 Wiley‐ Liss, Inc.
Comparison of immunoreactivity of neu oncoprotein in fine‐needle aspirates and paraffin‐embedded materials
Authors: Linder James , Martin Alvin W. , Davey Diane D. 
Published: February 2007

AbstractExpression of the neu oncogene has been extensively examined in frozen and paraffin section breast cancers; however, very few studies examine neu oncoprotein expression in fine‐needle aspirates. to this effect, we compared the expression of neu oncoprotein in formalin‐fixed paraffin‐embedded breast cancers and the corresponding fine‐needle aspirates of these cancers. There was 100% correlation between the expression of neu oncoprotein in the paraffin‐embedded breast cancers and the fine‐needle aspirates, indicating the suitability of fine‐needle aspirates for the expression of neu oncoprotein in breast cancers. © 1995 Wiley‐ Liss, Inc.
Role of AgNOR in diagnosis of thyroid follicular neoplasms on fine‐needle aspiration smears
Authors: Karmakar T. , Dey P. 
Published: February 2007

AbstractThis is a retrospective study to examine the role of AgNOR in differentiating thyroid follicular carcinoma and adenoma preoperatively on fine‐needle aspiration (FNA) smears. Nineteen histopathology‐proven cases of follicular adenoma and carcinoma were selected for this study. May‐Grünwald‐Giemsa—stained smears were destined and AgNOR staining technique was employed. Significant differences of AgNOR values were observed among follicular carcinoma versus adenoma (P < .05) and follicular carcinoma versus control cases (P < .01). Thereby the AgNOR technique may have some role in differentiating between benign and malignant follicular neoplasms of thyroid. Diagn Cytopathol 1995;12:148–151. © 1995 Wiley‐ Liss, Inc.
Primary lymphoma of bone: Diagnosis by fine‐needle aspiration biopsy in a pediatric patient
Authors: Niemann Theodore H. , Thomas Patricia A. 
Published: February 2007

AbstractWe present an unusual case of a child with an isolated lytic lesion in the L3 vertebral body. Fine‐needle aspiration biopsy showed a small, blue‐cell neoplasm raising the differential diagnosis of Ewing's sarcoma and lymphoblastic lymphoma. Through a combination of morphologic and immunofluorescent findings we established a diagnosis of lymphoblastic lymphoma. This case further documents the utility of fine‐needle aspiration biopsy in the diagnosis of pediatric tumors. © 1995 Wiley‐ Liss, Inc.
Cytodiagnostic pitfalls in pulmonary coccidioidomycosis
Authors: Chen Karl T. K. 
Published: February 2007

AbstractTwo cases of pulmonary coccidioidomycosis which were initially misdiagnosed as carcinoma in cytologic examinations of material obtained by bronchoscopy or fine‐needle aspiration are reported. the cytodiagnostic pitfalls of misinterpreting reactive bronchial epithelial cells as carcinoma cells and of failing to recognize fungal organisms are discussed. © 1995 Wiley‐ Liss, Inc.
Comparison of conventional and thinprep preparations of mucoid cytology samples
Authors: Hees K. , Lebeau P. B. 
Published: February 2007

AbstractCytological specimens containing large amounts of mucus and/or blood are often a considerable problem as far as preparation and especially screening are concerned. the ThinPrep™ ‐method, which uses a mucolytic and hemolysing solution and prepares slides automatically, eliminates difficulties in both areas. Comparing ThinPreps to conventionally prepared samples reveals equivalent or even superior cellular preservation. Cells are spread in a thin layer, mostly with only a minimal amount of residual mucus or blood. Screening is consequently much easier and faster. the time‐saving effect does not compromise the diagnostic accuracy, as we could demonstrate with an 92% overall agreement in both procedures. the difference of 8% could possibly be attributed to inadequate splitting of the samples. © 1995 Wiley‐ Liss, Inc.
Fine‐needle aspiration biopsy and imprint cytology of small non‐cleaved cell (Burkitt's) lymphoma
Authors: Stastny Janet F. , Almeida Margarida M. , Wakely Paul E. , Kornstein Michael J. , Frable William J. 
Published: February 2007

AbstractWe reviewed 13 cases of small non‐cleaved cell (Burkitt's) lymphoma diagnosed by fine‐needle aspiration biopsy (FNAB) and four cases where that diagnosis was made from touch imprints from surgical biopsy specimens. the most common site was intraabdominal. Histologic confirmation was present in 14/17 cases. General microscopic features on smears included high cellularity and an individual cell pattern. Extracellular lymphoglandular bodies were abundant and tingible body macrophages were prominent. Nuclei were intermediate in size and round with a finely dispersed chromatin pattern on May‐Grünwald‐Giemsa stained smears. Cytoplasm was basophilic and scant with prominent vacuoles. Extracellular vacuoles and vacuoles within lymphoglandular bodies occurred in all patients. Immunocytochemistry performed on 16/17 cases demonstrated a B cell phenotype in 15. Nine cases were kappa light chain positive and six were lambda light chain positive. Flow cytometry performed in five cases demonstrated diploid cells with a high S phase. We conclude that Burkitt's lymphoma can be diagnosed using combined FNAB and immunophenotyping. © Wiley‐Liss, Inc.