bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of human gingival fibroblast adhesion, morphology, tyrosine phosphorylation, and ERK 1/2 localization on polished, grooved and SLA substratum topographies
Authors: Kokubu Eitoyo , Hamilton Douglas W. , Inoue Takashi , Brunette Donald M. 
Published: November 2008

AbstractAttachment of connective tissue to dental implants, which is influenced by surface topography, is an important determinant of implant success. Approaches employed to alter topography include acid etching or blasting to produce roughened surfaces, and production of precisely defined topographies using microfabrication techniques. The aim of this study was to assess the influence of polished, microgrooved, and sand‐blasted, large grit, acid‐etched (SLA) topographies on fibroblast adhesion, morphology, activation, and ERK 1/2 phosphorylation and localization. Human gingival fibroblasts (HGFs) spread on all tested surfaces within 2 h, and topography influenced the pattern of phosphotyrosine localization. Fibrillar adhesion formation was prominent in HGFs cultured on microgrooves and SLA at 24 h compared with smooth. No significant difference in ERK 1/2 phosphorylation was observed at 2 or 24 h, but nuclear localization depended on culture time and substratum topography. Nuclear localization of ERK 1/2 occurred at 2 h on polished surfaces, but was not evident at 1 week. In contrast, cells on SLA and grooved surfaces did not exhibit nuclear localization of ERK 1/2 at early times, but did at 1 week. The results of this study suggest that rough and microfabricated topographies influence fibroblast adhesion and intracellular signaling through focal adhesion/integrin‐dependent mechanisms in a time‐dependent manner. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
Evaluation of the effect of four self‐etching adhesives ondentin permeability
Authors: Orucoglu Hasan , Belli Sema 
Published: November 2008

AbstractObjective:The aim of this study was to evaluate the effect of two two‐step and two single‐step self‐etching adhesives on dentin permeability using a computerized fluid filtration (CFF) test method.Methodology:Forty recently extracted sound human molar teeth were sectioned at the mid level of the crown to obtain dentin discs of 1 ± 0.2 mm. A total of 40 discs were obtained and randomly divided into four groups (n = 10). The permeability of the discs was measured using the CFF test method before and after application of the following self‐etching adhesives: Hybrid Bond, G‐Bond, AdheSE, and Protect Bond. Fluid movement measurements were made at 2‐min intervals for 8 min, and a mean of the values obtained was calculated for each specimen.Results:The in vitro fluid conductance of dentin discs treated with Hybrid Bond and AdheSE were lower than the dentin discs treated with G‐Bond and Protect Bond (p < 0.05).Conclusion:1‐Treating dentin discs with self‐etching adhesives caused significant reduction of dentin permeability. 2‐All self‐etching adhesives allowed diffusion of distilled water. However, AdheSE and Hybrid Bond showed the lowest permeability. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Predictive parameters for granulocyte colony‐stimulating factor‐induced peripheral blood stem cell mobilization
Authors: Okano Akira , Ashihara Eishi , Shimazaki Chihiro , Uchiyama Hitoji , Inaba Tohru , Taniguchi Kyoko , Maekawa Taira , Taniwaki Masafumi 
Published: November 2008

AbstractTo improve the selection of donors for allogeneic stem cell transplantation, it is important to identify reliable parameters that predict CD34+‐cell yields after granulocyte‐colony stimulating factor (G‐CSF)‐induced peripheral blood stem cell (PBSC) mobilization. We retrospectively investigated the peripheral blood (PB) kinetics of white blood cells (WBCs), CD34+ cells, matrix metalloproteinases (MMP)‐9 and ‐2, and tissue inhibitors of metalloproteinases (TIMP)‐1 and ‐2 in 15 healthy donors during their treatment with G‐CSF. All donors received 10 μg/kg of recombinant human G‐CSF once a day subcutaneously. Leukapheresis was initiated after 4 days of G‐CSF treatment, and G‐CSF treatment continued until the last day of leukapheresis. WBC and CD34+ cell numbers in the PB rose after 2 and 3 or 4 days of G‐CSF treatment, respectively. The PB CD34+ cell numbers on day 4 correlated weakly with the increase in WBC counts from day 1 to day 2 (R2 = 0.254, P = 0.056). There were also positive correlations between the CD34+ cell numbers in the PBSC products on day 4 and the CD34+ cells in the PB on days 1 and 4 (R2 = 0.768, P < 0.0001 and R2 = 0.816, P < 0.0005, respectively). The MMP‐9 plasma levels on days 1 and 4 also correlated positively with the day 4 circulating CD34+ cell numbers (R2 = 0.393, P < 0.05 and R2 = 0.406, P = 0.01, respectively). In conclusion, the CD34+ cell numbers in the PB steady state may be a useful parameter selecting allogeneic PBSC donors. J. Clin. Apheresis, 2008. © 2008 Wiley‐Liss, Inc.
Linear augmented Slater‐type orbital method for free standing clusters
Authors: Kang K. S. , Davenport J. W. , Glimm J. , Keyes D. E. , McGuigan M. 
Published: November 2008

AbstractWe have developed a Scalable Linear Augmented Slater‐Type Orbital (LASTO) method for electronic‐structure calculations on free‐standing atomic clusters. As with other linear methods we solve the Schrödinger equation using a mixed basis set consisting of numerical functions inside atom‐centered spheres and matched onto tail functions outside. The tail functions are Slater‐type orbitals, which are localized, exponentially decaying functions. To solve the Poisson equation between spheres, we use a finite difference method replacing the rapidly varying charge density inside the spheres with a smoothed density with the same multipole moments. We use multigrid techniques on the mesh, which yields the Coulomb potential on the spheres and in turn defines the potential inside via a Dirichlet problem. To solve the linear eigen‐problem, we use ScaLAPACK, a well‐developed package to solve large eigensystems with dense matrices. We have tested the method on small clusters of palladium. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
Prediction and analysis of binding affinities for chemically diverse HIV‐1 PR inhibitors by the modified SAFE_p approach
Authors: Arenas Miguel , Villaverde M. Carmen , Sussman Fredy 
Published: November 2008

AbstractOne of the biggest challenges in the “in silico” screening of enzyme ligands is to have a protocol that could predict the ligand binding free energies. In our group we have developed a very simple screening function (referred to as solvent accessibility free energy of binding predictor, SAFE_p) which we have applied previously to the study of peptidic HIV‐1 protease (HIV‐1 PR) inhibitors and later to cyclic urea type HIV‐1 PR inhibitors. In this work, we have extended the SAFE_p protocol to a chemically diverse set of HIV‐1 PR inhibitors with binding constants that differ by several orders of magnitude. The resulting function is able to reproduce the ranking and in many cases the value of the inhibitor binding affinities for the HIV‐1 PR, with accuracy comparable with that of costlier protocols. We also demonstrate that the binding pocket SAFE_p analysis can contribute to the understanding of the physical forces that participate in ligand binding. The analysis tools afforded by our protocol have allowed us to identify an induced fit phenomena mediated by the inhibitor and have demonstrated that larger fragments do not necessarily contribute the most to the binding free energy, an outcome partially brought about by the substantial role the desolvation penalty plays in the energetics of binding. Finally, we have revisited the effect of the Asp dyad protonation state on the predicted binding affinities. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
A combined semiempirical‐DFT study of oligomers within the finite‐chain approximation, evolution from oligomers to polymers
Authors: Derosa Pedro A. 
Published: November 2008

AbstractA computationally cheap approach combining time‐independent density functional theory (TIDFT) and semiempirical methods with an appropriate extrapolation procedure is proposed to accurately estimate geometrical and electronic properties of conjugated polymers using just a small set of oligomers. The highest occupied molecular orbital–lowest unoccupied molecular orbital gap (HLG) obtained at a TIDFT level (B3PW91) for two polymers, trans‐polyacetylene ‐the simplest conjugated polymer, and a much larger poly(2‐methoxy‐5‐(2,9‐ethyl‐hexyloxy)‐1,4‐phenylenevinylene (MEH‐PPV) polymer converge to virtually the same asymptotic value than the excitation energy obtained with time‐dependent DFT (TDDFT) calculations using the same functional. For TIDFT geometries, the HLG is found to converge to a value within the experimentally accepted range for the band gap of these polymers, when an exponential extrapolation is used; however if semiempirical geometries are used, a linear fit of the HLG versus 1/n is found to produce the best results. Geometrical parameters are observed to reach a saturation value in good agreement with experimental information, within the length of oligomers calculated here and no extrapolation was considered necessary. Finally, the performance of three different semiempirical methods (AM1, PM3, and MNDO) and for the TIDFT calculations, the performance of 7 different full electron basis sets (6‐311+G**, 6‐31+ +G**, 6‐311+ +G**, 6‐31+G**, 6‐31G**, 6‐31+G*, and 6‐31G) is compared and it is determined that the choice of semiempirical method or the basis set does not significantly affect the results. © 2008 Wiley Periodicals, Inc. J Comput Chem 2009
Radiosynthesis of [18F]PBR111, a selective radioligand for imaging the translocator protein (18 kDa) with PET
Authors: Dollé Frédéric , Hinnen Françoise , Damont Annelaure , Kuhnast Bertrand , Fookes Christopher , Pham Tien , Tavitian Bertrand , Katsifis Andrew 
Published: November 2008

AbstractPBR111 (2‐(6‐chloro‐2‐(4‐(3‐fluoropropoxy)phenyl)imidazo[1,2‐a]pyridin‐3‐yl)‐N,N‐diethylacetamide) is a novel, reported, high‐affinity and selective ligand for the translocator protein (18 kDa). PBR111 has been labelled with fluorine‐18 (half‐life: 109.8 min) using our Zymate‐XP robotic system. The process involves (A) a simple one‐step tosyloxy‐for‐fluorine nucleophilic aliphatic substitution (performed at 165°C for 5 min in DMSO using K[18F]F‐Kryptofix®222 and 6.8–7.6 µmol of the corresponding tosylate as precursor for labelling) followed by (B) C‐18 PrepSep cartridge pre‐purification and (C) semi‐preparative HPLC purification on a Waters Symmetry® C‐18. Up to 4.8 GBq (130 mCi) of [18F]PBR111 could be obtained with specific radioactivities ranging from 74 to 148 GBq/µmol (2–4 Ci/µmol) in 75–80 min (HPLC purification and SepPak®‐based formulation included), starting from a 37.0 GBq (1.0 Ci) [18F]fluoride batch. Overall non‐decay‐corrected isolated yields were 8–13% (13–21% decay‐corrected). Copyright © 2008 John Wiley & Sons, Ltd.
Assessment of fibula osteoseptocutaneous flap donor‐site morbidity using balance and gait test
Authors: Chou Shih‐Wei , Liao Han‐Tsung , Yazar Sukru , Lin Chih‐Hung , Lin Yin‐Chou , Wei Fu‐Chan 
Published: November 2008

AbstractTo assess the functional deficits in gait and balance after fibular osteoseptocutaneous flap harvest, a case–control study in a hospital‐based biomechanical laboratory was conducted. Eleven patients receiving fibula osteoseptocutaneous flaps were enrolled as study group and 10 age‐matched and healthy subjects as a control group. The average postoperative period at the time of evaluation was 27.4 months (range: 10 to 68 months) and the average age at the time of evaluation was 52.1 years (range: 38 to 76 years). The balance test by Smart Balance Master and gait analysis were used for objective assessment of the donor leg function postoperatively. No statistical differences were observed in the kinetic and kinematic variables of gait analysis except double support time in both the groups. There were also no significant differences in simple static balance test (eyes open, eyes closed, swaying vision, eyes open with swaying surface, and sway‐referenced vision and support). Significant differences were observed in more difficult task (eye closed with swaying support) and in the use of ankle strategy for maintaining maximum balance. In the dynamic balance test only specificity of the forward left direction determined significant difference in reaction time of movement control of gravity. The results of ankle strategy usage and dynamic balance indicated deficits of ankle muscle torque. Necessary postoperative physical training is required for ankle muscle torque and vestibular sense. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 555–560, 2009
BMP‐7 protects against progression of cartilage degeneration after impact injury
Authors: Hurtig Mark , Chubinskaya Susan , Dickey Jim , Rueger David 
Published: November 2008

AbstractIn vivo studies were used to characterize a model of cartilage injury leading to osteoarthritis progression in the medial femorotibial joint of sheep. In three subsequent studies, bilateral impact injuries were created and one joint received intraarticular injections of 340 µg of rhBMP‐7 protein in a collagen particle carrier while the contralateral knee received the vehicle alone. Sheep were allocated to three groups that received intraarticular injections on day 0 (group A), 21 (group B), or 90 (group C) after experimental knee injury. In each group the, joints were evaluated for signs of osteoarthritis progression 90 days after the last treatment using India ink stained area, OARSI histological scoring, cartilage sGAG content, immunostaining for apoptosis (TUNEL), caspase‐3, collagen degradation (Col 2 3/4C short collagen epitope), and the endogenous (pro‐) form of BMP‐7 protein. Knee joints that received rhBMP‐7 immediately after injury had small focal lesions at the injury site that did not progress into the surrounding cartilage. Joints that received BMP‐7 3 weeks after injury were improved and had limited progression compared to controls, but joints that received the protein 12 weeks after injury had no statistically significant improvement. These studies suggest that BMP‐7 may be chondroprotective after traumatic injury in patients if it is administered within 3 to 4 weeks of the index injury. The mechanism of protection after sublethal injury appeared to be an increased survival of chondrocytes that are able to participate in the repair process. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 602–611, 2009
Mechanical characterization of external fixator stiffness for a rat femoral fracture model
Authors: Willie Bettina , Adkins Kyle , Zheng Xing , Simon Ulrich , Claes Lutz 
Published: November 2008

AbstractClinical and experimental studies have shown that several mechanical factors influence the fracture healing process. One such factor, interfragmentary movement, is affected by loading and the stiffness of the fixation device. This study evaluated the stiffness of different external fixation devices for a rat femoral fracture model, using in vitro and analytical methods. The contribution to the stiffness of the fixation construct was dominated by the flexibility of the pins in relation to their offset, diameter, and material properties. The axial stiffness increased with decreasing offset and increasing pin diameter. Titanium pins resulted in significantly lower axial stiffness compared to stainless steel pins of the same design. The fixator body material and fixator length had a less pronounced influence on fixation stiffness. Mechanically characterized external fixation devices will allow in vivo study of the fracture healing process utilizing pre‐calculated fracture fixation stiffness. These characterized fixation devices will allow controlled manipulation of the axial and shear interfragmentary movement to achieve a flexible fixation resulting in callus formation compared to a more rigid fixation limiting callus formation in a rat femoral fracture model. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 687–693, 2009
Long‐term effects of saw osteotomy versus random fracturing on bone healing and remodeling in a sheep tibia model
Authors: Dumont Clemens , Kauer Fritz , Bohr Stefan , Schmidtmann Ulrich , Knopp Werner , Engelhardt Thomas , Stürmer Ewa Klara , Stürmer Klaus Michael 
Published: November 2008

AbstractThis article is about the evaluation of possible differences in biomechanical or histomorphological properties of bone healing between saw osteotomy and random fracturing after 6 months. A standardized, 30° oblique monocortical saw osteotomy of sheep tibia was carried out, followed by manual fracture completion of the opposed cortical bone. Fixation was performed by bridge plating (4.5 mm, LCDCP, broad). X‐rays were taken immediately after surgery and at the end of the study. Polychrome fluorescent staining was performed according to a standardized protocol in the 2nd, 4th 6th, 10th, 14th, 18th, 22th and 26th week. Ten sheep were comprehensively evaluated. Data for stiffness and histomorphology are reported. The average bending stiffness of the operated bone was higher (1.7 (SD 0.3) with plate (MP) vs. 1.5 without plate) than for the intact bone (1.4 (SD 0.2), though no significant differences in bending stiffness were observed (P>0.05). Fluorescence staining revealed small numbers of blood vessels and less fragment resorption and remodeling in the osteotomy gap. Bone healing after saw osteotomy shows a very close resemblance to ‘normal’ fracture healing. However, vascular density, fragment resorption, fragment remodeling, and callus remodeling are reduced at the osteotomy. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27: 680–686, 2009
Development of a novel pomegranate standard and new method for the quantitative measurement of pomegranate polyphenols
Authors: Martin Keith R. , Krueger Christian G. , Rodriquez Gerardo , Dreher Mark , Reed Jess D. 
Published: November 2008

AbstractBACKGROUND: The Folin–Ciocalteu method is routinely used to calculate total polyphenol concentrations (TPP) in food products. However, the use of inappropriate standards such as gallic acid (GA) may give inaccurate estimates of TPP content. A method for the production of a pomegranate (Punica granatum L. ‘Wonderful’) standard was developed and validated to avoid this problem. The PPS was produced by solid phase (C18) isolation of polyphenols from POMx powder (a nutritional supplement) and used for TPP analysis.RESULTS: TPP content of POMx was > 90% using PPS, whereas the GA standard underestimated TPP by up to 30%. Compositional analysis confirmed a balance of about 9% non‐polyphenolic compounds. Mass spectrometry analysis corroborated polyphenol composition and confirmed pomegranate‐specific peaks, i.e., punicalagin and punicalin.CONCLUSION: Results support the use of pomegranate‐specific standards to accurately determine TPP. Copyright © 2008 Society of Chemical Industry
2‐Furoylmethyl amino acids, hydroxymethylfurfural, carbohydrates and β‐carotene as quality markers of dehydrated carrots
Authors: Soria Ana C , Olano Agustin , Frías Juana , Peñas Elena , Villamiel Mar 
Published: November 2008

AbstractBACKGROUND: Processing of vegetables in the food industry usually includes dehydration as a preservation process. Industrial convective air drying of carrot can involve steam blanching of the raw product after peeling and cutting, and different stages of dehydration (first space, second space and final drying). Although the shelf‐life of carrot is significantly extended, important changes in its chemical composition can take place during dehydration since high temperatures and long times are used. This research is a preliminary study to evaluate the usefulness of β‐carotene, carbohydrates, hydroxymethylfurfural (HMF) and 2‐furoylmethyl amino acids (2‐FM‐AA) as quality markers of dehydrated carrots.RESULTS: A considerable decrease in β‐carotene and reducing carbohydrates was observed during dehydration. HMF, absent in raw carrots, increased during the whole drying process and the highest formation was found during the steam blanching stage. 2‐FM‐AA of lysine, arginine, γ‐aminobutyric acid and alanine were progressively originated up to the second space and decreased during the final drying.CONCLUSION: The combined use of HMF and 2‐FM‐AA seems to be advantageous for the assessment of the optimal processing conditions to obtain high‐quality dehydrated carrots. Copyright © 2008 Society of Chemical Industry
Characterization of α‐tubulin gene distinctively presented in a cytoplasmic male sterile and its maintainer line of non‐heading Chinese cabbage
Authors: Zhang Jingyi , Li Ying , Shi Gongjun , Chen Xiaofeng , Wang Jianjun , Hou Xilin 
Published: November 2008

AbstractBACKGROUND: Microtubules are prominent components of the cytoskeleton in every eukaryotic cell. Plant microtubules are essential for a wide variety of cellular functions, including generation of cell polarity, intracellular transport, positioning of organelles, cell wall deposition and cell division. The major component of microtubules is tubulin, an α,β heterodimer protein with a molecular mass of each subunit of around 50 kDa. Tubulin exists in cells as a mixture of polypeptides differing in their isoelectric points. Some post‐translational modifications of tubulins are thought to modulate the functions and localization of microtubules within the cell.RESULTS: The complete sequence of a single‐copy α‐tubulin gene Tuba1, belonging to a multiple gene family of non‐heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), was obtained. The gene was expressed in high levels in young leaves and stamens, and it was also highly expressed during all stages of microsporogenesis in the maintainer. However, there was a distinct difference in α‐tubulin expression between the sterile stage and the normal stages of pollen in a cytoplasmic male sterility line and its maintainer.CONCLUSION: Tuba1 was significantly related to the cell division and elongation of non‐heading Chinese cabbage, demonstrating that this gene played an important role in the development of pollen and may be closely related to male sterility. Copyright © 2008 Society of Chemical Industry
Electrospun Micro‐ and Nanostructured Polymer Particles
Authors: Liu Jing , Rasheed Asif , Dong Hongming , Carr Wallace W. , Dadmun Mark D. , Kumar Satish 
Published: November 2008

AbstractElectrospinning at relatively low polymer concentrations produces polymer particles rather than fibers. To study the relationship between solvent characteristics and particle morphologies, PMMA was electrospun from seven different solvents and PSVPh random copolymers were electrospun from solutions in MEK. High‐speed photography was used to visualize the particle‐formation process. Based on these studies, a qualitative relationship between the solvent properties and the electrospun particle morphologies is discussed. By tailoring the solution properties and electrospinning conditions, particles with different morphologies (porous polygonal particles, solid polygonal particles, hollow spheres, cups etc) can be produced.magnified image