bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spiral packing and chiral selectivity in model membranes probed by phase-resolved sum-frequency generation microscopy
Authors: Fellows Alexander P. , John Ben , Wolf Martin , Thämer Martin 
Published: April 2024

AbstractSince the lipid raft model was developed at the end of the last century, it became clear that the specific molecular arrangements of phospholipid assemblies within a membrane have profound implications in a vast range of physiological functions. Studies of such condensed lipid islands in model systems using fluorescence and Brewster angle microscopies have shown a wide range of sizes and morphologies, with suggestions of substantial in-plane molecular anisotropy and mesoscopic structural chirality. Whilst these variations can significantly alter many membrane properties including its fluidity, permeability and molecular recognition, the details of the in-plane molecular orientations underlying these traits remain largely unknown. Here, we use phase-resolved sum-frequency generation microscopy on model membranes of mixed chirality phospholipid monolayers to fully determine the three-dimensional molecular structure of the constituent micron-scale condensed domains. We find that the domains possess curved molecular directionality with spiralling mesoscopic packing, where both the molecular and spiral turning directions depend on the lipid chirality, but form structures clearly deviating from mirror symmetry for different enantiomeric mixtures. This demonstrates strong enantioselectivity in the domain growth process and indicates fundamental thermodynamic differences between homo- and heterochiral membranes, which may be relevant in the evolution of homochirality in all living organisms.
Genomic spectrum of actionable alterations in serial cell free DNA (cfDNA) analysis of patients with metastatic breast cancer
Authors: Bar Yael , Keenan Jennifer C. , Niemierko Andrzej , Medford Arielle J. , Isakoff Steven J. , Ellisen Leif W. , Bardia Aditya , Vidula Neelima 
Published: April 2024

AbstractWe aimed to study the incidence and genomic spectrum of actionable alterations (AA) detected in serial cfDNA collections from patients with metastatic breast cancer (MBC). Patients with MBC who underwent plasma-based cfDNA testing (Guardant360®) between 2015 and 2021 at an academic institution were included. For patients with serial draws, new pathogenic alterations in each draw were classified as actionable alterations (AA) if they met ESCAT I or II criteria of the ESMO Scale for Clinical Actionability of Molecular Targets (ESCAT). A total of 344 patients with hormone receptor-positive (HR+)/HER2-negative (HER2-) MBC, 95 patients with triple-negative (TN) MBC and 42 patients with HER2-positive (HER2 + ) MBC had a baseline (BL) cfDNA draw. Of these, 139 HR+/HER2-, 33 TN and 13 HER2+ patients underwent subsequent cfDNA draws. In the HR+/HER2- cohort, the proportion of patients with new AA decreased from 63% at BL to 27–33% in the 2nd-4th draws (p < 0.0001). While some of the new AA in subsequent draws from patients with HR+/HER2- MBC were new actionable variants in the same genes that were known to be altered in previous draws, 10-24% of patients had new AA in previously unaltered genes. The incidence of new AA also decreased with subsequent draws in the TN and HER2+ cohorts (TN: 25% to 0–9%, HER2 + : 38% to 14–15%). While the incidence of new AA in serial cfDNA decreased with subsequent draws across all MBC subtypes, new alterations with a potential impact on treatment selection continued to emerge, particularly for patients with HR+/HER2- MBC.
Kirigami-inspired, three-dimensional piezoelectric pressure sensors assembled by compressive buckling
Authors: Zhang Yi , Liu Changbo , Jia Ben , Ma Dongqin , Tian Xuecheng , Cui Yuanyuan , Deng Yuan 
Published: April 2024

AbstractPiezoelectric sensors whose sensing performances can be flexibly regulated hold significant promise for efficient signal-acquisition applications in the healthcare field. The existing methods for regulating the properties of polyvinylidene fluoride (PVDF) films mainly include material modification and structural design. Compared to material modification, which has a long test period and an unstable preparation process, structural design is a more efficient method. The irigami structure combined with compressive buckling can endow the flexible film with rich macrostructural features. Here, a method is fabricated to modulate the sensing performance by employing distinct 3D structures and encapsulation materials with varying Young’s moduli. The relationship among the aspect ratio (α), pattern factor (η), elastic modulus of encapsulation materials, and equivalent stiffness is obtained by finite element simulation, which provides theoretical guidance for the design of the 2D precursor and the selection of encapsulation materials. In the demonstration applications, the sensor accurately captures pulse waveforms in multiple parts of the human body and is employed for the pressure monitoring of different parts of the sole under various posture states. This method of structure design is efficient, and the preparation process is convenient, providing a strategy for the performance control of piezoelectric pressure sensors.
Variations to plasma H2O2 levels and TAC in chronical medicated and treatment-resistant male schizophrenia patients: Correlations with psychopathology
Authors: Yang Haidong , Sun Wenxi , Yang Man , Li Jin , Zhang Jing , Zhang Xiaobin 
Published: April 2024

AbstractAccumulating evidence suggests that imbalanced oxidative stress (OS) may contribute to the mechanism of schizophrenia. The aim of the present study was to evaluate the associations of OS parameters with psychopathological symptoms in male chronically medicated schizophrenia (CMS) and treatment-resistant schizophrenia (TRS) patients. Levels of hydrogen peroxide (H2O2), hydroxyl radical (·OH), peroxidase (POD), α-tocopherol (α-toc), total antioxidant capacity (TAC), matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were assayed in males with CMS and TRS, and matched healthy controls. Schizophrenia symptoms were assessed using the Positive and Negative Syndrome Scale (PANSS). The results demonstrated significant differences in the variables H2O2 (F = 5.068, p = 0.008), ·OH (F = 31.856, p < 0.001), POD (F = 14.043, p < 0.001), α-toc (F = 3.711, p = 0.027), TAC (F = 24.098, p < 0.001), and MMP-9 (F = 3.219, p = 0.043) between TRS and CMS patients and healthy controls. For TRS patients, H2O2 levels were correlated to the PANSS positive subscale (r = 0.386, p = 0.032) and smoking (r = −0,412, p = 0.021), while TAC was significantly negatively correlated to the PANSS total score (r = −0.578, p = 0.001) and POD and TAC levels were positively correlated to body mass index (r = 0.412 and 0.357, p = 0.021 and 0.049, respectively). For patients with CMS, ·OH levels and TAC were positively correlated to the PANSS general subscale (r = 0.308, p = 0.031) and negatively correlated to the PANSS total score (r = −0.543, p < 0.001). Furthermore, H2O2, α-toc, and ·OH may be protective factors against TRS, and POD was a risk factor. Patients with CMS and TRS exhibit an imbalance in OS, thus warranting future investigations.
A pan-genome of 69 Arabidopsis thaliana accessions reveals a conserved genome structure throughout the global species range
Authors: Lian Qichao , Huettel Bruno , Walkemeier Birgit , Mayjonade Baptiste , Lopez-Roques Céline , Gil Lisa , Roux Fabrice , Schneeberger Korbinian , Mercier Raphael 
Published: April 2024

AbstractAlthough originally primarily a system for functional biology, Arabidopsis thaliana has, owing to its broad geographical distribution and adaptation to diverse environments, developed into a powerful model in population genomics. Here we present chromosome-level genome assemblies of 69 accessions from a global species range. We found that genomic colinearity is very conserved, even among geographically and genetically distant accessions. Along chromosome arms, megabase-scale rearrangements are rare and typically present only in a single accession. This indicates that the karyotype is quasi-fixed and that rearrangements in chromosome arms are counter-selected. Centromeric regions display higher structural dynamics, and divergences in core centromeres account for most of the genome size variations. Pan-genome analyses uncovered 32,986 distinct gene families, 60% being present in all accessions and 40% appearing to be dispensable, including 18% private to a single accession, indicating unexplored genic diversity. These 69 new Arabidopsis thaliana genome assemblies will empower future genetic research.
The BALA project: A pioneering monitoring of Azorean forest invertebrates over two decades (1999–2022)
Authors: Pozsgai Gabor , Lhoumeau Sébastien , Amorim Isabel R. , Boieiro Mário , Cardoso Pedro , Costa Ricardo , Ferreira Maria Teresa , Leite Abrão , Malumbres-Olarte Jagoba , Oyarzabal Guilherme , Rigal François , Ros-Prieto Alejandra , Santos Ana M. C. , Gabriel Rosalina , Borges Paulo A. V. 
Published: April 2024

AbstractGlobally, there is a concerning decline in many insect populations, and this trend likely extends to all arthropods, potentially impacting unique island biota. Native non-endemic and endemic species on islands are under threat due to habitat destruction, with the introduction of exotic, and potentially invasive, species, further contributing to this decline. While long-term studies of plants and vertebrate fauna are available, long-term arthropod datasets are limited, hindering comparisons with better-studied taxa. The Biodiversity of Arthropods of the Laurisilva of the Azores (BALA) project has allowed gathering comprehensive data since 1997 in the Azorean Islands (Portugal), using standardised sampling methods across islands. The dataset includes arthropod counts from epigean (pitfall traps) and canopy-dwelling (beating samples) communities, enriched with species information, biogeographic origins, and IUCN categories. Metadata associated with the sample protocol and events, like sample identifier, archive number, sampled tree species, and trap type are also recorded. The database is available in multiple formats, including Darwin Core, which facilitates the ecological analysis of pressing environmental concerns, such as arthropod population declines and biological invasions.
Existence of reservoir with finite-dimensional output for universal reservoir computing
Authors: Sugiura Shuhei , Ariizumi Ryo , Asai Toru , Azuma Shun-ichi 
Published: April 2024

AbstractIn this paper, we prove the existence of a reservoir that has a finite-dimensional output and makes the reservoir computing model universal. Reservoir computing is a method for dynamical system approximation that trains the static part of a model but fixes the dynamical part called the reservoir. Hence, reservoir computing has the advantage of training models with a low computational cost. Moreover, fixed reservoirs can be implemented as physical systems. Such reservoirs have attracted attention in terms of computation speed and energy consumption. The universality of a reservoir computing model is its ability to approximate an arbitrary system with arbitrary accuracy. Two sufficient reservoir conditions to make the model universal have been proposed. The first is the combination of fading memory and the separation property. The second is the neighborhood separation property, which we proposed recently. To date, it has been unknown whether a reservoir with a finite-dimensional output can satisfy these conditions. In this study, we prove that no reservoir with a finite-dimensional output satisfies the former condition. By contrast, we propose a single output reservoir that satisfies the latter condition. This implies that, for any dimension, a reservoir making the model universal exists with the output of that specified dimension. These results clarify the practical importance of our proposed conditions.
Spectral characterization of the impact of modifiers and different prepare temperatures on snow lotus medicinal residue-biochar and dissolved organic matter
Authors: Zhang Sha , Sun Zenghong , Yao Yanna , Wang Xinyu , Tian Shuge 
Published: April 2024

AbstractThis study involved the production of 20 biochar samples derived from secondary medicinal residues of Snow Lotus Oral Liquid, processed within the temperature range of 200–600 °C. Additionally, four medicinal residues, including dissolved organic matter (DOM), from 24 samples obtained using the shaking method, served as the primary source material. The investigation focused on two key factors: the modifier and preparation temperature. These factors were examined to elucidate the spectral characteristics and chemical properties of the pharmaceutical residues, biochar, and DOM. To analyze the alterations in the spectral attributes of biochar and medicinal residues, we employed near-infrared spectroscopy (NIR) in conjunction with Fourier-infrared one-dimensional and two-dimensional correlation spectroscopy. These findings revealed that modifiers enhanced the aromaticity of biochar, and the influence of preparation temperature on biochar was diminished. This observation indicates the stability of the aromatic functional group structure. Comparative analysis indicated that Na2CO3 had a more pronounced structural effect on biochar, which is consistent with its adsorption properties. Furthermore, we utilized the fluorescence indices from UV–visible spectroscopy and excitation-emission-matrix spectra with the PARAFAC model to elucidate the characteristics of the fluorescence components in the DOM released from the samples. The results demonstrated that the DOM released from biochar primarily originated externally. Aromaticity reduction and increased decay will enhance the ability of the biochar to bind pollutants. Those results confirmed the link between the substantial increase in the adsorption performance of the high-temperature modified charcoal in the previous study and the structural changes in the biochar. We investigated the structural changes of biochar and derivative DOM in the presence of two perturbing factors, modifier and preparation temperature. Suitable modifiers were selected. Preparation for the study of adsorption properties of snow lotus medicinal residues.
Next-generation sequencing of host genetics risk factors associated with COVID-19 severity and long-COVID in Colombian population
Authors: Angulo-Aguado Mariana , Carrillo-Martinez Juan Camilo , Contreras-Bravo Nora Constanza , Morel Adrien , Parra-Abaunza Katherine , Usaquén William , Fonseca-Mendoza Dora Janeth , Ortega-Recalde Oscar 
Published: April 2024

AbstractCoronavirus disease 2019 (COVID-19) was considered a major public health burden worldwide. Multiple studies have shown that susceptibility to severe infections and the development of long-term symptoms is significantly influenced by viral and host factors. These findings have highlighted the potential of host genetic markers to identify high-risk individuals and develop target interventions to reduce morbimortality. Despite its importance, genetic host factors remain largely understudied in Latin-American populations. Using a case–control design and a custom next-generation sequencing (NGS) panel encompassing 81 genetic variants and 74 genes previously associated with COVID-19 severity and long-COVID, we analyzed 56 individuals with asymptomatic or mild COVID-19 and 56 severe and critical cases. In agreement with previous studies, our results support the association between several clinical variables, including male sex, obesity and common symptoms like cough and dyspnea, and severe COVID-19. Remarkably, thirteen genetic variants showed an association with COVID-19 severity. Among these variants, rs11385942 (p < 0.01; OR = 10.88; 95% CI = 1.36–86.51) located in the LZTFL1 gene, and rs35775079 (p = 0.02; OR = 8.53; 95% CI = 1.05–69.45) located in CCR3 showed the strongest associations. Various respiratory and systemic symptoms, along with the rs8178521 variant (p < 0.01; OR = 2.51; 95% CI = 1.27–4.94) in the IL10RB gene, were significantly associated with the presence of long-COVID. The results of the predictive model comparison showed that the mixed model, which incorporates genetic and non-genetic variables, outperforms clinical and genetic models. To our knowledge, this is the first study in Colombia and Latin-America proposing a predictive model for COVID-19 severity and long-COVID based on genomic analysis. Our study highlights the usefulness of genomic approaches to studying host genetic risk factors in specific populations. The methodology used allowed us to validate several genetic variants previously associated with COVID-19 severity and long-COVID. Finally, the integrated model illustrates the importance of considering genetic factors in precision medicine of infectious diseases.
Spatial–temporal evolution and peer effects of urban green development efficiency in China
Authors: Zhou Jialiang , Zhong Mingchun 
Published: April 2024

AbstractIn the process of global urban development, there are urgent ecological security and environmental pollution problems, green development is the fundamental way for urban sustainable development, economic transformation and mitigation of ecological and environmental problems. Based on the panel data of 283 cities at prefecture level and above in China from 2003 to 2017, this paper analyzes spatial–temporal evolution characteristics of urban green development efficiency (UGDE) and the peer effects of UGDE between cities of different grades. It is found that during the study period, in terms of temporal evolution, the average UGDE in China increased from 0.47 in 2003 to 0.61 in 2017, with a cumulative growth rate of 29.79%, showing a rising trend in general. In terms of spatial evolution, the number of low-efficiency cities and medium-efficiency cities continued to decrease. The eastern region has always been the main distribution area of higher-efficiency cities and high-efficiency cities; in the central region, UGDE in most cities improved significantly; in the western region, UGDE has always lagged behind that in the eastern and central regions. In addition, the center of gravity of UGDE presented a trend of northwest migration in general, with a total displacement of 100.07 km, and UGDE showed a spatial dispersion trend. The empirical results indicate that the improvement of UGDE in large cities has a driving effect on that in neighboring medium cities and small cities through the positive peer effect, and the growth of UGDE in medium cities has a promoting effect on that in neighboring small cities through the positive peer effect; the increase of UGDE in medium cities has a positive peer effect on that in neighboring large cities, and the growth of UGDE in small cities has a positive peer effect on that in neighboring medium cities; UGDE promotes each other between large cities through the positive peer effect.
Effects of decongestion on nasal cavity air conditioning efficiency: a CFD cohort study
Authors: Xiao Qiwei , Bates Alister J. , Doorly Denis J. 
Published: April 2024

AbstractDecongestion reduces blood flow in the nasal turbinates, enlarging the airway lumen. Although the enlarged airspace reduces the trans-nasal inspiratory pressure drop, symptoms of nasal obstruction may relate to nasal cavity air-conditioning. Thus, it is necessary to quantify the efficiency of nasal cavity conditioning of the inhaled air. This study quantifies both overall and regional nasal air-conditioning in a cohort of 10 healthy subjects using computational fluid dynamics simulations before and after nasal decongestion. The 3D virtual geometry model was segmented from magnetic resonance images (MRI). Each subject was under two MRI acquisitions before and after the decongestion condition. The effects of decongestion on nasal cavity air conditioning efficiency were modelled at two inspiratory flowrates: 15 and 30 L min−1 to represent restful and light exercise conditions. Results show inhaled air was both heated and humidified up to 90% of alveolar conditions at the posterior septum. The air-conditioning efficiency of the nasal cavity remained nearly constant between nostril and posterior septum but dropped significantly after posterior septum. In summary, nasal cavity decongestion not only reduces inhaled air added heat by 23% and added moisture content by 19%, but also reduces the air-conditioning efficiency by 35% on average.
A framework for selecting and assessing soil quality indicators for sustainable soil management in waste dumps
Authors: Li Yue , Zhao Hongbao , Liu Jiashun , chaonan Chen , Yuxuan Guo 
Published: April 2024

AbstractThe primary objective of this study was to develop soil quality indexes (SQIs) to reveal the changes in SQ during the restoration of vegetation in the reclaimed waste dumps of the Hequ open-pit coal mine. The study built an SQI evaluation model for waste dumps based on the soil management assessment framework. The total data set (TDS) consisted of nine physicochemical property indicators. The selection of the minimum data set (MDS) involved the utilization of principal component analysis (PCA) and Norm values. The SQ was comprehensively evaluated for nine indicators, taking into account the non-linear membership function and the improved Nemerow index. The findings suggested a notable disparity in the SQ between the reclaimed area and the unreclaimed area, yet the overall SQ fell short. In the TDS index system, the organic matter has the highest weight and a greater contribution to the soil quality of the waste dumps. In the MDS indicator system, the weights of organic matter and total nitrogen are both 0.5. According to Nemerow index method, the average SQIN of 5 plots is calculated to be 0.4352 ± 0.194. The average value obtained from TDS is 0.581 ± 0.236, and the average value obtained from MDS is 0.602 ± 0.351. The weighted additive method was employed to compute three SQIs, all of which yielded satisfactory outcomes. And the above evaluation methods indicate that the overall soil quality level of the waste dumps is at a moderate level. The sequence of SQ in various waste dumps was as follows: No.4lower > No.1 > No.2 > No.3 > No.4upper. Specifically, the non-linear membership function indicated that pH, available nitrogen (AN), available phosphorus (AP), surface moisture content (SMC), and bulk density (BD) were crucial in limiting SQIs in total waste dumps. The crucial limiting SQIs in unreclaimed areas were total phosphorus (TP) and total nitrogen (TN). This analysis demonstrates its efficacy in formulating strategies for the SQ evaluation and targeted soil reclamation plans of waste dumps.