bluuebunny/crossref_metadata_2025_split huggingface dataset:
SUMO regulates the cytoplasmonuclear transport of its target protein Daxx
Authors: Chen Angela , Wang Ping‐Yao , Yang Yu‐Chih , Huang Yi‐Hsin , Yeh Jeng‐Jung , Chou Yu‐Huai , Cheng Jiin‐Tsuey , Hong Yi‐Ren , Li Steven S.‐L. 
Published: February 2006

AbstractIt is known that Fas death domain‐associated protein (Daxx) possesses both putative nuclear and cytoplasmic functions. However, the nuclear transport mechanism is largely unknown. This study examined the nuclear location signal (NLS) of Daxx and whether the nuclear transport of Daxx was mediated by small ubiquitin‐related modifier (SUMO). Two NLS motifs of Daxx, leucine (L)‐rich nuclear export signal (NES)‐like motif (188IXXLXXLLXL197) and C‐terminal lysine (K) rich NLS2 (amino acids 627–634) motif, were identified and the K630 and K631 on the NLS2 motif were characterized as the major sumoylation sites of Daxx by in vitro sumoylation analysis. Proteins of inactive SUMO (SUMO‐Δ), a sumoylation‐incompetent mutant, and Daxx NLS mutants (Daxx‐NESmut and Daxx NLS2mut) were dispersed in cytoplasm. The cytoplasmic dispersed Daxx mutants could be relocalized to nucleus by cotransfection with active SUMO, but not with inactive SUMO‐Δ, demonstrating the role of SUMO on regulating the cytoplasmonuclear transport of Daxx. However, inactive SUMO‐Δ could also be relocalized to nucleus during cotransfection with wild‐type Daxx, suggesting that SUMO regulation of the cytoplasmonuclear transport of its target protein Daxx does not need covalent modification. This study shows that cytoplasmic SUMO has a biological role in enhancing the cytoplasmonuclear transport of its target protein Daxx and it may be done through the non‐sumoylation interactions. J. Cell. Biochem. 98: 895–911, 2006. © 2006 Wiley‐Liss, Inc.
Role of soluble ceruloplasmin in iron uptake by midbrain and hippocampus neurons
Authors: Ke Ya , Ho Kwokping , Du Jinrong , Zhu Li , Xu Youjia , Wang Qin , Wang Chen Yuen , Li Lianzhi , Ge Xiaohu , Chang Yang Zhong , Qian Zhong Ming 
Published: February 2006

AbstractCeruloplasmin (CP) is essential for brain iron homeostasis. However, its precise function in brain iron transport has not been definitely determined. In this study, we investigated the effects of soluble CP on iron influx and efflux in primary neuronal culture from the midbrain (the substantia nigra and striatum) and the hippocampus. Our data showed that low concentrations of CP (2, 4, 8 µg/ml) can promote iron influx into iron‐deficient neurons, but not the neurons with normal iron status. The same concentrations of CP had no effect on iron efflux from iron‐sufficient and normal‐iron neurons. Contrary to our expectation, we did not find any regional difference in the effects of CP on iron influx as well as efflux in neurons. The changes in quenching (iron influx) and also dequenching (iron efflux) of intracellular fluorescence, induced by the addition of CP with iron, in the midbrain neurons were no different from those in the hippocampus neurons. The data showed that soluble CP has a role in iron uptake by iron‐deficient brain neurons under our experimental conditions. The physiological significance of the results forms the focus for future work. J. Cell. Biochem. 98: 912–919, 2006. © 2006 Wiley‐Liss, Inc.
Identification of nuclear localization signal that governs nuclear import of BRD7 and its essential roles in inhibiting cell cycle progression
Authors: Zhou Ming , Liu Huaying , Xu Xiaojie , Zhou Houde , Li Xiaoling , Peng Cong , Shen Shourong , Xiong Wei , Ma Jian , Zeng Zhaoyang , Fang Songqing , Nie Xinmin , Yang Yixin , Zhou Jie , Xiang Juanjuan , Cao Li , Peng Shuping , Li Shufang , Li Guiyuan 
Published: February 2006

AbstractBRD7, a novel bromodomain gene, is identified to be associated with nasopharyngeal carcinoma (NPC). Decreased or loss of expression of BRD7 was detected in NPC biopsies and cell lines. Overexpression of BRD7 could inhibit NPC cell growth and arrest cells in cell cycle by transcriptionally regulating some important molecules involved in ras/MEK/ERK and Rb/E2F pathway, and downregulate the promoter activity of E2F3. In the present study, the subcellular localization of BRD7 was investigated. It was found that BRD7 was mainly localized in nucleus without distinct cell‐specific difference between COS7 and HNE1. Furthermore, a functional nuclear localization signal (NLS) sequence ranging from amino acid 65 to 96 was identified and characterized. The NLS is composed of a cluster of four bipartite nuclear targeting sequences, which are tightly linked and extremely overlapped. We found that whether the entire NLS or the four bipartite nuclear targeting sequences could respectively determine the nuclear import of green fluorescent protein (GFP). The most important is that NLS‐deleted BRD7 shifted the nuclear localization to be mostly in cytoplasm, and failed or reduced to negatively regulate the expression of cell cycle related molecules, cyclin D1 and E2F3, and cell cycle progression from G1 to S phase. In conclusion, NLS is an essential motif affecting BRD7 nuclear distribution, and the nuclear localization of BRD7 is critical for the expression of cell cycle related molecules and cell biological function. J. Cell. Biochem. 98: 920–930, 2006. © 2006 Wiley‐Liss, Inc.
The vertebrate E1/U17 small nucleolar ribonucleoprotein particle
Authors: Eliceiri George L. 
Published: February 2006

AbstractEach of the many different box H/ACA ribonucleoprotein particles (RNPs) present in eukaryotes and archaea consists of four common core proteins and one specific H/ACA small RNA, which bears the sequence elements H (ANANNA) and ACA. Most of the H/ACA RNPs are small nucleolar RNPs (snoRNPs), which are localized in nucleoli, and are one of the two major classes of snoRNPs. Most H/ACA RNPs direct pseudouridine synthesis in pre‐rRNA and other RNAs. One H/ACA small nucleolar RNA (snoRNA), vertebrate E1/U17 (snR30 in yeast), is required for pre‐rRNA cleavage processing that generates mature 18S rRNA. E1 snoRNA is encoded in introns of protein‐coding genes, and the evidence suggests that human E1 RNA undergoes uridine insertional RNA editing. The vertebrate E1 RNA consensus secondary structure shows several features that are absent in other box H/ACA snoRNAs. The available UV‐induced RNA‐protein crosslinking results suggest that the E1 snoRNP is asymmetrical in vertebrate cells, in contrast to other H/ACA snoRNPs. The vertebrate E1 snoRNP in cells is surprisingly complex: (i) E1 RNA contacts directly and specifically several proteins which do not appear to be any of the H/ACA RNP four core proteins; and (ii) multiple E1 RNA sites are needed for E1 snoRNP formation, E1 RNA stability, and E1 RNA–protein direct interactions. J. Cell. Biochem. 98: 486–495, 2006. © 2006 Wiley‐Liss, Inc.
Role of protein phosphatase 2A in the regulation of endothelial cell cytoskeleton structure
Authors: Tar Krisztina , Csortos Csilla , Czikora Istvan , Olah Gabor , Ma Shwu‐Fan , Wadgaonkar Raj , Gergely Pal , Garcia Joe G.N. , Verin Alexander D. 
Published: February 2006

AbstractOur recently published data suggested the involvement of protein phosphatase 2A (PP2A) in endothelial cell (EC) barrier regulation (Tar et al. [2004] J Cell Biochem 92:534–546). In order to further elucidate the role of PP2A in the regulation of EC cytoskeleton and permeability, PP2A catalytic (PP2Ac) and A regulatory (PP2Aa) subunits were cloned and human pulmonary arterial EC (HPAEC) were transfected with PP2A mammalian expression constructs or infected with PP2A recombinant adenoviruses. Immunostaining of PP2Ac or of PP2Aa + c overexpressing HPAEC indicated actin cytoskeleton rearrangement. PP2A overexpression hindered or at least dramatically reduced thrombin‐ or nocodazole‐induced F‐actin stress fiber formation and microtubule (MT) dissolution. Accordingly, it also attenuated thrombin‐ or nocodazole‐induced decrease in transendothelial electrical resistance indicative of barrier protection. Inhibition of PP2A by okadaic acid abolished its effect on agonist‐induced changes in EC cytoskeleton; this indicates a critical role of PP2A activity in EC cytoskeletal maintenance. The overexpression of PP2A significantly attenuated thrombin‐ or nocodazole‐induced phosphorylation of HSP27 and tau, two cytoskeletal proteins, which potentially could be involved in agonist‐induced cytoskeletal rearrangement and in the increase of permeability. PP2A‐mediated dephosphorylation of HSP27 and tau correlated with PP2A‐induced preservation of EC cytoskeleton and barrier maintenance. Collectively, our observations clearly demonstrate the crucial role of PP2A in EC barrier protection. J. Cell. Biochem. 98: 931–953, 2006. © 2006 Wiley‐Liss, Inc.
Paneling human thyroid cancer cell lines for candidate proteins for targeted anti‐angiogenic therapy
Authors: Hoffmann Sebastian , Wunderlich Anette , Celik Ilhan , Maschuw Katja , Hassan Iyad , Hofbauer Lorenz C. , Zielke Andreas 
Published: February 2006

AbstractTumor angiogenesis is believed to result from an imbalance of pro‐ and anti‐angiogenic factors, some of which are candidates for targeted therapy. Such therapy has raised hopes for patients with undifferentiated thyroid carcinomas, who are facing a grave prognosis with a survival of only months. In this study, in vivo growth of xenografted human thyroid carcinomas unexpectedly responded quite differently to neutralizing anti‐vascular endothelial growth factor (VEGF) antibody. In particular, lasting inhibition as well as accelerated growth occurred after treatment. Consequently, a panel of anti‐angiogenic factors was addressed in a representative sample of thyroid carcinoma lines. VEGF, fibroblast growth factor (FGF‐2), and endostatin were demonstrated by Western blotting and EIA, whereas PDGF‐A, PDGF‐B, and IL‐6 were negative. Quantification of VEGF, FGF‐2, and endostatin revealed a wide range of concentrations from 500 to 4,200 pg/ml VEGF, 5 to 60 pg/ml FGF‐2, and 50 to 300 pg/ml endostatin, not related to a particular histologic thyroid carcinoma background. Angiostatin (kringles 1–3) was detected in all, but one of the cell lines. Finally, aaATIII was confirmed in FTC133 cells. These data highlight the complex regulation of angiogenesis in thyroid carcinoma cell lines and suggest that the array of angiogenic factors differs markedly between individual cell lines. For the first time, angiostatin, endostatin, and possibly also aaATIII are identified as novel candidate regulators of angiogenesis in thyroid carcinoma cells. J. Cell. Biochem. 98: 954–965, 2006. © 2006 Wiley‐Liss, Inc.
Gap junction communications influence upon fibroblast synthesis of Type I collagen and fibronectin
Authors: Ehrlich H. Paul , Sun Bonnie , Saggers Gregory C. , Kromath Fatuma 
Published: February 2006

AbstractIn rats polyvinyl alcohol sponge subcutaneous implants treated with gap junctional intercellular communications (GJIC) uncouplers showed reduced deposition of connective tissue. Do uncouplers inhibit the synthesis and deposition of a new connective tissue by fibroblasts? Confluent human dermal fibroblasts in serum‐free medium received either endosulfan or oleamide, GJIC uncouplers. Collected media were subjected to Dot Blot analysis for native Type I collagen and fibronectin. Uncoupler‐treated fibroblasts released less Type I collagen, while there was no change in fibronectin release. Collagen synthesis was restored to normal, when the uncouplers were removed, showing that these uncouplers were reversible and not toxic to cells. Northern blot analysis revealed procollagen α1 (I) mRNA was minimally affected by endosulfan. Oleamide‐treated 17‐day chick embryo calvaria explants were incubated with Type I collagen antibody, frozen, cryosectioned, and then subjected to rhodamine (Rh) tagged anti‐mouse‐IgG antibody, to detect newly deposited Type I collagen. Fluorescent antibody‐collagen complexes were localized on the periphery of cells in control calvaria, but absent around cells in oleamide‐treated calvaria. GJIC optimize collagen synthesis but not fibronectin synthesis. The lack of connective tissue deposited in granulation tissues treated with uncouplers appears related to the inhibition of collagen synthesis. These findings suggest that altering GJIC might control collagen deposition in scarring. J. Cell. Biochem. 98: 735–743, 2006. © 2006 Wiley‐Liss, Inc.
Theoretical mechanistic study on the radical‐molecule reaction of CHCl2/CCl3 with NO2
Authors: Zhang Jia‐Xu , Li Ze‐Sheng , Liu Jing‐Yao , Sun Chia‐Chung 
Published: February 2006

AbstractThe radical‐molecule reaction mechanism of CHCl2 and CCl3 with NO2 have been explored theoretically at the B3LYP/6‐311G(d,p) and MC‐QCISD (single‐point) levels. For the singlet potential energy surface (PES) of CHCl2 + NO2 reaction, the association of CHCl2 with NO2 was found to be a barrierless carbon‐to‐nitrogen approach forming an energy‐rich adduct a (HCl2CNO2) followed by isomerization to b1 (trans–cis‐HCl2CONO), which can easily interconvert to b2, b3, and b4. Subsequently, the most feasible pathway is the 1,3‐chlorine migration associated with NO1 bond cleavage of b1 leading to P1 (CHClO + ClNO). The second competitive pathway is the 1,4‐chlorine migration along with NO1 bond rupture of b4 giving rise to P2 (CHClO + ClON). Moreover, some of P1 and P2 can further dissociate to give P6 (CHClO + Cl + NO). The lesser followed competitive channel is the 1,3‐H‐shift from C to N atom along with NO1 bond rupture of b1 to form P3 (CCl2O + HNO). The concerted 1,4‐H‐shift accompanied by NO1 bond fission of b3 to product P4 (CCl2O + HON) is even much less feasible. For the singlet PES of CCl3 + NO2 reaction, the only primary product is found to be P1 (CCl2O + ClNO), which can lead to P2 (CCl2O + Cl + NO) via dissociation of ClNO. The obtained major products CHClO and CCl2O for CHCl2 + NO2 and CCl3 + NO2 reactions, respectively, are in good agreement with kinetic detection in experiment. Compared with the singlet pathways, the triplet pathways may have less contributions to both reactions. Because the rate‐determining transition state involved in the feasible pathways lie above the reactants R, the title reactions may be important in high‐temperature processes. The similarities and discrepancies among the CHnCl3−n + NO2 (n == 0–2) reactions are discussed in terms of the substitution effect. The present study may be helpful for further experimental investigation of the title reactions. © 2006 Wiley Periodicals, Inc. J Comput Chem 27: 661–671, 2006
Repression of HIP/RPL29 expression induces differentiation in colon cancer cells
Authors: Liu Jian‐Jun , Huang Bao Hua , Zhang Jinqiu , Carson Daniel D. , Hooi Shing Chuan 
Published: February 2006

AbstractWe had previously shown that the expression of heparin/heparan sulfate interacting protein/ribosomal protein L29 (HIP/RPL29) was upregulated in colon cancer tissues. The present study investigated the role of HIP/RPL29 in differentiation in colon cancer cells. Inducing cellular differentiation in HT‐29 cells by both sodium butyrate and glucose deprivation resulted in a significant downregulation of HIP/RPL29 expression. The β‐catenin/Tcf‐4 pathway is the most important pathway controlling the switch between cellular differentiation and proliferation in intestinal epithelial cells. Inducing differentiation by dominant‐negative inhibition of the β‐catenin/Tcf‐4 complexes in LS174T cells also resulted in downregulation of HIP/RPL29. To determine whether a lower expression of HIP/RPL29 could induce differentiation in cancer cells, small interfering RNA (siRNA) targeting HIP/RPL29 was transfected into LS174T cells. The resultant knockdown of HIP/RPL29 expression induced cellular differentiation, as shown by the increased expression of two known markers of differentiation in LS174T cells, galectin‐4 and mucin‐2. In addition, the differentiation process induced by repression of HIP/RPL29 expression was accompanied by the upregulation of p21 and p53. In conclusion, HIP/RPL29 plays a role in the cellular differentiation process in colon cancer cells. The differentiation process is at least partially mediated by the upregulation of p21 and p53 pathways. J. Cell. Physiol. 207: 287–292, 2006. © 2006 Wiley‐Liss, Inc.
Morphometric discrimination of early life stage Lampetra tridentata and L. richardsoni (Petromyzonidae) from the Columbia River Basin
Authors: Meeuwig Michael H. , Bayer Jennifer M. , Reiche Rebecca A. 
Published: February 2006

AbstractThe effectiveness of morphometric and meristic characteristics for taxonomic discrimination of Lampetra tridentata and L. richardsoni (Petromyzonidae) during embryological, prolarval, and early larval stages (i.e., age class 1) were examined. Mean chorion diameter increased with time from fertilization to hatch and was significantly greater for L. tridentata than for L. richardsoni at 1, 8, and 15 days postfertilization. Lampetra tridentata larvae had significantly more trunk myomeres than L. richardsoni; however, trunk myomere numbers were highly variable within species and deviated from previously published data. Multivariate examinations of prolarval and larval L. tridentata (7.2–11.0 mm; standard length) and L. richardsoni (6.6–10.8 mm) were conducted based on standard length and truss element lengths established from eight homologous landmarks. Principal components analysis indicated allometric relationships among the morphometric characteristics examined. Changes in body shape were indicated by groupings of morphometric characteristics associated with body regions (e.g., oral hood, branchial region, trunk region, and tail region). Discriminant function analysis using morphometric characteristics was successful in classifying a large proportion (>94.7%) of the lampreys sampled. J. Morphol. © 2006 Wiley‐Liss, Inc.
The assessment of communities of practice
Authors: Verburg Robert M. , Andriessen J. H. Erik 
Published: February 2006

AbstractThis paper introduces the Community Assessment Toolkit (CAT) as a method for the assessment of Communities of Practice (CoPs) and provides an overview of its construction and applicability. It shows that the methodology is based on current theories with regard to CoPs and group dynamics. The method was also tested in practice through a pilot and a study among members of 7 communities of practice (N=271) in order to enable reliability tests and scale analysis. The method is unique in the sense that it provides feedback on the overall performance of CoPs on the individual, group and organizational level. The systematic nature of the CAT questionnaire enables comparisons between communities both within and between organizations. The CAT may be used for further scientific research into CoPs as well as for the analysis of community effectiveness for companies. Copyright © 2006 John Wiley & Sons, Ltd.
Valuing knowledge sharing in Lafarge
Authors: Perrin Alexandre , Vidal Pascal , McGill Jennifer 
Published: February 2006

AbstractThis paper describes the detailed process of a knowledge sharing strategy at Lafarge, a global player in the construction materials industry. The case study explains why this company uses such a strategy to create value for stakeholders, provide a local access to know‐how and build a knowledge sharing culture among divisions. It argues that a well‐articulated approach of a knowledge sharing strategy consists in creating a knowledge portfolio, supporting a knowledge management structure, providing tools for collaboration and nurture a culture of knowledge sharing through awards. At the end, we discuss the lessons learned by the Corporate Knowledge Manager on this strategy and we study how she can quantify the value brought by its action. In a whole, this paper gives insights of critical issues in moving a global company towards a knowledge‐sharing organization. Copyright © 2006 John Wiley & Sons, Ltd.
Knowledge asset value spiral: linking knowledge assets to company's performance
Authors: Carlucci Daniela , Schiuma Giovanni 
Published: February 2006

AbstractIn today's economy, more and more organisations are realising that they must explicitly manage their knowledge resources to acquire and maintain their competitive advantage. For this reason, managers need to better understand how they can identify and evaluate existing knowledge resources within an organisation and how to manage them in order to achieve competitive advantage.Many organisations frequently embark on Knowledge Management (KM) initiatives without a clear idea of what business benefits they could expect. This is mainly due to lack of understanding of the links between KM and company's business performance. Understanding these links supports the validation of KM investment and can help explain what knowledge should be managed within an organisation to achieve performance improvement. It appears from literature analysis, that there is not straightforward link between KM and company's performance but rather a complex relationship. In such a prospect, the main research question that has inspired this study is: ‘How can an organisation plan and evaluate a KM initiative designed to improve company's performances?’ On the base of this question, this paper provides a framework—the Knowledge Assets Value Spiral (KAVS)—which focuses on how to apply KM initiatives in a step by step process to achieve company's performance improvements. The framework provides guidelines to plan, implement and evaluate KM initiatives that are linked to performance objectives. It is illustrated how organisations can identify their key knowledge assets as well as their interdependencies and better understand their role in causal dynamics in company's value delivering. The application of the Analytic Hierarchy Process (AHP) method is proposed and a case example describing the application of the framework is illustrated. Copyright © 2006 John Wiley & Sons, Ltd.
Centralized versus peer‐to‐peer knowledge management systems
Authors: Maier Ronald , Hädrich Thomas 
Published: February 2006

AbstractThe term knowledge management system (KMS) has been used widely to denote information and communication technologies in support of knowledge management. However, so far investigations about the notion of KMS, their functions and architecture as well as the differences to other types of systems remain on an abstract level. This paper reviews the literature on KMS and distills a number of characteristics concerning the specifics of knowledge to be managed, the platform metaphor, advanced services, KM instruments, supported processes, participants and goals of their application. The paper then presents two ideal architectures for KMS, a centralized and a peer‐to‐peer architecture, discusses their differences with the help of two example systems and suggests that each of these architectures fits a different type of KM initiative. Copyright © 2006 John Wiley & Sons, Ltd.
Cognitive function in multiple system atrophy of the cerebellar type
Authors: Bürk Katrin , Daum Irene , Rüb Udo 
Published: February 2006

AbstractDementia represents an exclusion criterion for the diagnosis of multiple system atrophy (MSA), but there have been reports of fronto‐executive dysfunction in patients with MSA of the striatonigral type (MSA‐P). To study the cognitive profile of MSA, 20 patients with MSA of the cerebellar type (MSA‐C) were subjected to an extensive neuropsychological test battery comprising tests for IQ, attention, verbal and visuospatial memory, as well as executive function. There was evidence for impaired verbal memory and verbal fluency. Test performance was not related to the severity of motor disability. Regarding the similar cognitive syndrome of MSA‐P, the otherwise subclinical problems in MSA‐C result from subcortical rather than from cerebellar dysfunction. © 2006 Movement Disorder Society