bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel terpenyl esters from Australian finger lime (Citrus australasica) peel extract
Authors: Delort Estelle , Jaquier Alain 
Published: March 2009

AbstractThe volatile constituents of the peel solvent extract of the Australian finger lime Citrus australasica were analysed by GC–MS. Besides limonene, isomenthone was a major component, which is rare in Citrus species. Six new terpenyl esters were also identified and confirmed by chemical synthesis: citronellyl 2‐methylbutanoate; 1,8(10)‐p‐menthadien‐9‐yl propanoate; 1,8(10)‐p‐menthadien‐9‐yl 2‐methylbutanoate; 1,8(10)‐p‐menthadien‐9‐yl 3‐methylbutanoate; 1‐p‐menthen‐9‐yl 2‐methylbutanoate; and 1‐p‐menthen‐9‐yl 3‐methylbutanoate. To the best of our knowledge, the last four compounds have never before been identified in a natural product extract. Copyright © 2009 John Wiley & Sons, Ltd.
Design characteristics that influence attrition in geriatric antidepressant trials: meta‐analysis
Authors: Heo Moonseong , Papademetriou Eros , Meyers Barnett S. 
Published: March 2009

AbstractObjectiveTo identify study‐level and treatment group‐level characteristics that are associated with attrition in antidepressant trials for the depressed elderly.MethodsThis meta‐analysis used 68 published antidepressant randomized trials for the elderly depressed. Study‐level and treatment group‐level variables were extracted. The former consisted of: use of placebo arm, number of arms, unbalanced treatment allocation, year conducted, study duration, study location, number of centers, and patient pool. The latter consisted of: assigned treatments, baseline mean Hamilton Depression Rating Scale (HDRS), baseline mean age, gender ratio, attrition size, and sample size. Univariate logistic regressions with attrition as the dependent variable were applied, followed by application of a forward stepwise selection method for identification of independent attrition correlates.ResultsThe 68 studies had a total of 153 treatment groups and 8,385 subjects. Among them, 2,287 subjects were terminated early, resulting in an overall 27.3% attrition rate. The stepwise results showed that higher attrition was significantly associated with active antidepressant groups as opposed to placebo groups, higher baseline HDRS, smaller sample size, unbalanced allocation of treatments, longer duration, and studies conducted in USA.ConclusionsThe attrition in geriatric antidepressant trials can be affected by study and group‐level design characteristics that include severity of depression symptoms and active treatments among others. Copyright © 2009 John Wiley & Sons, Ltd.
Mixed medullary‐papillary carcinoma of the thyroid: Report of a case and review of the literature
Authors: Nangue Charlette , Bron Luc , Portmann Luc , Volante Marco , Ris Hans‐Beat , Monnier Philippe , Andrejevic‐Blant Snezana 
Published: March 2009

AbstractBackground.Mixed medullary‐follicular thyroid carcinoma denotes a rare and heterogeneous group of tumors displaying morphological and immunophenotypical features of both origins within the same lesion.Method.We report a case of a 41‐year‐old woman with a lump in the right side of the neck, increasing in pain and size over several weeks. Serum levels of calcitonine (1140 ng/L) and carcinoembryonic antigen (288 μg/L) were very high. Fine‐needle aspiration cytology suggested a diagnosis of medullary thyroid carcinoma. Total thyroidectomy, along with bilateral functional neck and mediastinal lymph‐node dissection, were performed.Results.The histopathological examination yielded a diagnosis of medullary carcinoma in the right thyroid lobe, closely intermingled with a nonencapsulated classical papillary carcinoma. One ipsilateral lymph node showed micrometastasis of the medullary counterpart.Conclusion.When compared with other cases reported in literature, this particular presentation should be recognized, if required, morphologic and functional criteria are used. The treatment is mostly surgical, driven by the medullary component. The presence of micrometastasis in 1 ipsilateral cervical lymph‐node underlines the importance of cervicomediastinal lymph‐node dissection and careful searching for metastatic disease. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Cavernous hemangioma of infrahyoid carotid sheath and review of the literature on carotid sheath tumors
Authors: Antonopoulos Constantine , Karagianni Maria , Zolindaki Crisoula , Anagnostou Emmanouil , Vagianos Constantine 
Published: March 2009

AbstractBackground.Carotid sheath may include a variety of lesions such as neurogenic or vascular, tumors originating from extra‐adrenal chromaphil cells, squamous cell carcinomas, and lymphomas. Hemangiomas are benign congenital neoplasms, which, when located in the head and neck region, may be easily misdiagnosed due to their infrequent localization.Methods and Results.We report an unusual case of cavernous hemangioma of the infrahyoid carotid sheath presenting as palpable mass in a 51‐year‐old woman with no prior medical history. A review of the literature was performed to investigate the radiological and histopathological characteristics and highlight the treatment of carotid sheath lesions.Conclusions.CT, MRI, and MR angiography would help to locate hemangiomas of carotid sheath, but only surgical excision and histopathology can reveal their true nature. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Endoscopic mucosal suturing of vocal fold with placement of stent for the treatment of glottic stenoses
Authors: Xu Wen , Han Demin , Hu Huiying , Chen Xuejun , Li Hongyan , Hou Lizhen , Zhang Li 
Published: March 2009

AbstractBackground.Stenosis of the glottis can influence both phonation and respiration. The management of laryngeal web is difficult because of the tendency of readhesion to recur locally.Methods.Twenty‐five patients with web from the anterior glottis to the entire membranous portion of the vocal folds were included. All patients underwent endoscopic mucosal suturing or combined with placement of laryngeal silastic sheet stents.Results.Eight patients underwent suture of the vocal fold alone. Seventeen patients underwent suture of the vocal fold combined with silastic sheet placement. The vocal assessment of the perceptual Grade, Roughness, Breathiness, Aesthenia, Strain (GRBAS) scale, Voice Handicap Index scores, and maximum phonation time are significantly improved after surgery. Vibration of the vocal folds is normal or close to normal in 23 patients. The patients who were initially tracheotomy dependent were all decannulated.Conclusion.Endoscopic suture of the vocal fold and placement of a laryngeal stent can make successful outcomes for laryngeal web. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Higher laryngeal preservation rate after CO2 laser surgery compared with radiotherapy in T1a glottic laryngeal carcinoma
Authors: Schrijvers Michiel L. , van Riel Eva L. , Langendijk Johannes A. , Dikkers Frederik G. , Schuuring Ed , van der Wal Jacqueline E. , A. M. van der Laan Bernard F. 
Published: March 2009

AbstractBackground.Clinical outcome of endoscopic CO2 laser surgery and radiotherapy in early‐stage glottic laryngeal carcinoma is difficult to compare because of differences in treatment selection and patient groups. Therefore, we compared local control, overall survival, and laryngeal preservation in a homogenous group of patients with T1a glottic carcinoma with normal/diminished mucosal wave treated with either CO2 laser surgery or radiotherapy.Methods.Retrospective survival analysis was performed on 100 patients with T1a glottic carcinoma treated with CO2 laser surgery (n = 49) or radiotherapy (n = 51), diagnosed at the University Medical Center Groningen between 1990 and 2004.Results.No significant differences in local control and overall survival were found. Ultimate 5‐year laryngeal preservation was significantly better in the CO2 laser surgery group (95% vs 77%, p = .043).Conclusion.Patients with T1a glottic carcinoma with normal/diminished mucosal wave treated with CO2 laser surgery had a significantly better laryngeal preservation rate than patients treated with radiotherapy. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Life course sun exposure and risk of prostate cancer: Population‐based nested case‐control study and meta‐analysis
Authors: Gilbert Rebecca , Metcalfe Chris , Oliver Steven E. , Whiteman David C. , Bain Chris , Ness Andy , Donovan Jenny , Hamdy Freddie , Neal David E. , Lane J. Athene , Martin Richard M. 
Published: March 2009

AbstractThere is currently no means of primary prevention for prostate cancer. Increased exposure to ultraviolet‐radiation may be protective, but the literature is inconclusive. We investigated associations of life course exposure to sunlight with prostate cancer. The study design was a UK‐wide nested case‐control study, based on 1,020 prostate specific antigen‐detected cases and 5,044 matched population controls and a systematic review with meta‐analysis. Men with olive/brown skin (OR = 1.47; 95% CI: 1.00 to 2.17), men who burnt rarely/never (OR = 1.11; 0.95 to 1.29) and men with the lowest levels of intense sun exposure in the 2 years prior to diagnosis (OR = 1.24; 1.03 to 1.50) had an increased prostate cancer risk. However, amongst men with prostate cancer, spending less time outside was associated with a reduced risk of advanced cancer (OR = 0.49; 0.27 to 0.89) and high Gleason grade (OR = 0.62; 0.43 to 0.91), and men who burnt rarely/never had a reduced risk of advanced cancer (OR = 0.71; 0.47 to 1.08). The meta‐analysis provided weak evidence that men with the lowest (versus highest) sunlight exposure had an increased prostate cancer risk (4 studies, random‐effects pooled relative risk = 1.13; 0.98 to 1.29) and higher advanced or fatal prostate cancer risk (6 studies, random‐effects pooled relative risk = 1.14; 0.98 to 1.33). Our data and meta‐analyses provide limited support for the hypothesis that increased exposure to sunlight may reduce prostate cancer risk. The findings warrant further investigation because of their implications for vitamin D chemoprevention trials. © 2009 UICC
Incorporating structural characteristics for identification of protein methylation sites
Authors: Shien Dray‐Ming , Lee Tzong‐Yi , Chang Wen‐Chi , Hsu Justin Bo‐Kai , Horng Jorng‐Tzong , Hsu Po‐Chiang , Wang Ting‐Yuan , Huang Hsien‐Da 
Published: March 2009

AbstractStudies over the last few years have identified protein methylation on histones and other proteins that are involved in the regulation of gene transcription. Several works have developed approaches to identify computationally the potential methylation sites on lysine and arginine. Studies of protein tertiary structure have demonstrated that the sites of protein methylation are preferentially in regions that are easily accessible. However, previous studies have not taken into account the solvent‐accessible surface area (ASA) that surrounds the methylation sites. This work presents a method named MASA that combines the support vector machine with the sequence and structural characteristics of proteins to identify methylation sites on lysine, arginine, glutamate, and asparagine. Since most experimental methylation sites are not associated with corresponding protein tertiary structures in the Protein Data Bank, the effective solvent‐accessible prediction tools have been adopted to determine the potential ASA values of amino acids in proteins. Evaluation of predictive performance by cross‐validation indicates that the ASA values around the methylation sites can improve the accuracy of prediction. Additionally, an independent test reveals that the prediction accuracies for methylated lysine and arginine are 80.8 and 85.0%, respectively. Finally, the proposed method is implemented as an effective system for identifying protein methylation sites. The developed web server is freely available at http://MASA.mbc.nctu.edu.tw/. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2009
The spermatogonial stem cell niche
Authors: De Rooij Dirk G. 
Published: March 2009

AbstractSpermatogonial stem cells (SSCs; As spermatogonia) and their direct descendants (Apr and Aal spermatogonia) are preferentially located in those areas of the seminiferous tubules that border on the interstitial tissue. Fewer of these cells are present in tubule areas directly bordering on another tubule. Therefore, the SSC niche is related to the presence of interstitial tissue. The somatic cells within the seminiferous tubules, the Sertoli cells, are able to produce growth factors that stimulate self‐renewal (GDNF, FGF2) and differentiation (activin A, BMP4, and SCF) of the SSCs. As Sertoli cells are everywhere on the basal membrane of the tubules, other factors coming from outside the tubules must determine, either directly or indirectly via Sertoli cells, whether in a particular area self‐renewal of SSCs will be preferred or differentiation in the form of Apr formation. Self‐renewal will be preferred in the stem cell niche and differentiation outside of the niche. Factors that could link the niche to the interstitial tissue are CSF1, produced by Leydig cells that stimulate stem cell proliferation and FSH, the concentration of which will be highest near blood vessels and that stimulates GDNF production by Sertoli cells. Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.
Pediatric hospitalists and primary care providers: A communication needs assessment
Authors: Harlan Gregory , Srivastava Rajendu , Harrison Lanny , McBride Glen , Maloney Christopher 
Published: March 2009

AbstractBACKGROUND/OBJECTIVE:Existing research on hospitalist–primary care provider (PCP) communication focuses mainly on adult hospitalist models with little known about the quality of current pediatric hospitalist‐PCP communication. Our objective was to perform a needs assessment by exploring important issues around communication between pediatric hospitalists and PCPs.METHODS:Six previously identified issues around hospitalist‐PCP communication from the adult hospitalist literature were abstracted and incorporated into an open‐ended and closed‐ended questionnaire. The questionnaire was pretested, revised, and administered by phone to 10 pediatric hospitalists and 12 pediatric PCPs residing in our 5‐state catchment area. Interviews were transcribed and openly coded, and themes compared using qualitative methods.RESULTS:The 6 identified issues were: quality of communication, barriers to communication, methods of information sharing, key data element requirements, critical timing, and perceived benefits. Hospitalists and PCPs rated overall quality of communication from “poor” to “very good.” Both groups acknowledge that significant barriers to optimal communication currently exist, yet the barriers differ for each group. Hospitalists and PCPs agree on what information is important to transmit (diagnoses, medications, follow‐up needs, and pending laboratory test results) and critical times for communication during the hospitalization (at discharge, admission, and during major clinical changes). Both groups also agree that optimal communication could improve many aspects of patient care.CONCLUSIONS:Identifying and addressing barriers to these 6 issues may help both hospitalists and PCPs implement targeted interventions aimed at improving communication. Future studies will need to demonstrate the link between improved hospitalist‐PCP communication and improved patient care and outcomes. Journal of Hospital Medicine 2009. © 2009 Society of Hospital Medicine.
Antioxidant properties of Korean black raspberry wines and their apoptotic effects on cancer cells
Authors: Jung Jaewook , Son Mi‐Young , Jung Seungwon , Nam Pilwoo , Sung Jung‐Suk , Lee Seung‐Joo , Lee Kwang‐Geun 
Published: March 2009

AbstractBACKGROUND: The objective of the study was to determine the antioxidant activities of Korean black raspberry concentrate (KBR: Rubus coreanus Miq.) and Korean black raspberry wine concentrates (KBRW‐1 and KBRW‐2) using the DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radical scavenging assay. The apoptotic effects of the KBRW concentrates on various cancer cells were also investigated.RESULTS: Both the KBR concentrate and KBRW concentrates showed dose‐related antioxidant activities in the DPPH assay. At concentrations of 500 µg mL−1 and 1000 µg mL−1, the KBR concentrate antioxidant activities were 59% and 79%, respectively. KBRW‐1 and KBRW‐2 inhibited radical formation at 500 µg mL−1 by 19% and 48%, respectively. The antioxidant activity of KBRW‐2 was comparable to various red wines (RW‐1, RW‐2, RW‐3, and RW‐4). However, at 1000 µg mL−1, the antioxidant activities of KBRW‐1 and KBRW‐2 were 53% and 83% that of α‐tocopherol, respectively. Both RW‐1 and KBRW‐2 showed marked antiproliferative effects on all cancer cell types, with decreases in cell viability greater than 50%. This was co‐related with apoptotic characteristics, including chromatin condensation and apoptotic body formation, as determined by cell morphological assessment. Cell cycle analysis showed that treating AGS cells with RW‐1 and KBRW‐2 resulted in 25% and 30% apoptotic cell accumulation, respectively. KBRW‐2 induced the cleavage of poly(ADP‐ribose) polymerase (PARP) and the activation of caspase‐3 within the AGS cells. Levels of PARP cleavage were increased five‐ and three‐fold by RW‐1 and KBRW‐2, respectively, and the level of caspase‐3 was significantly increased by the treatment with KBRW‐2.CONCLUSION: Overall, the results of this study suggest that Korean black raspberry wine displays antioxidant activity comparable to that of imported red wines, and has anticancer effects that may be attributed to induction of the apoptotic pathway. Copyright © 2009 Society of Chemical Industry
Crosstalk between adipose‐derived stem/stromal cells and vocal fold fibroblasts in vitro
Authors: Kumai Yoshihiko , Kobler James B. , Park Hyoungshin , Lopez‐Guerra Gerardo , Karajanagi Sandeep , Herrera Victoria L.M. , Zeitels Steven M. 
Published: March 2009

AbstractObjectives/Hypothesis:To explore adipose‐derived stem cell/fibroblast interactions as a potential remodeling pathway for vocal fold scar.Study Design:Fibroblasts and adipose‐derived stem/stromal cells (ASCs) were cultured alone and in combination in a cell‐contact–independent paracrine system. Analyses of cell proliferation, and the production of hyaluronic acid (HA) and collagen were performed on samples collected on days 1, 3, and 7.Methods:Normal fibroblasts (NFs) were isolated bilaterally from the subepithelial lamina propria of two normal ferret vocal folds. Scar fibroblasts (SFs) were isolated from vocal folds that were electrocauterized 2 weeks before harvest. ASCs were isolated from lipoaspirated subcutaneous abdominal fat of two ferrets. A transwell cell‐contact–independent cell communication culturing system was used for coculture experiments. Cells were seeded at 50,000/well in both monoculture and coculture experiments.Results:In monoculture, SFs proliferated faster and produced less HA and more collagen than NFs at day 7 (P < .05). In SF/ASC coculture, SF proliferation was diminished and collagen production at day 7 decreased (P < .05). HA production did not differ between monoculture and coculture conditions.Conclusions:Normal and scar‐tissue–derived vocal fold fibroblasts maintain phenotypic differences in culture, thus validating this in vitro scar model. In co‐culture, contact‐independent crosstalk occurs between SFs and ASCa, leading to less collagen secretion. The data support the hypothesis that ASCs can induce favorable remodeling of scarred vocal folds in vivo by their interactions with endogenous fibroblasts. Laryngoscope, 2009
New Preparation of Structurally Symmetric, Biodegradable Poly(L‐lactide) Disulfides and PLLA–Stabilized, Photoluminescent CdSe Quantum Dots
Authors: Hou Xiaodong , Li Qiaobo , Jia Lin , Li Yang , Zhu Yingdan , Cao Amin 
Published: March 2009

AbstractNew, biodegradable poly(L‐lactide) disulfides, PLLA‐SS‐PLLA, were first prepared through the DMAP‐catalyzed ring‐opening polymerization of L‐lactide with a dihydroxyethyl disulfide initiator, and were further catalytically reduced into thiol‐end‐functionalized poly(L‐lactide)s, HO‐PLLA‐SH, with a tributyl phosphine catalyst (PBu3). Employing the HO‐PLLA‐SH as the ligand, new core‐shell CdSe/PLLA quantum dots (QDs) were continuously prepared via a facile ligand‐exchanging process with the CdSe/TOPO QD precursor. The chemical structures, morphologies and solvent solubility of these prepared CdSe/PLLA QDs were investigated by NMR spectroscopy, FTIR spectroscopy, XRD, TEM and excitation under either room light or UV radiation at 365 nm, demonstrating the successful ligand replacement and the new formation of core‐shell CdSe/PLLA QDs (diameter:4.0 ± 0.3 nm). Finally, UV and FL results indicate the two factors of the HO‐PLLA‐SH ligand molecular weight and the ligand/QD precursor feeding weight ratio were important for preparing stable and highly photoluminescent CdSe/PLLA QDs.magnified image
Synthesis of 12,12‐Ammonium Ionenes with Functionality for Chain Extension and Cross‐Linking via UV Irradiation
Authors: Williams Sharlene R. , Barta Zsófia , Ramirez Sean M. , Long Timothy E. 
Published: March 2009

AbstractA novel synthetic methodology for preparing chain‐extended and cross‐linked ionenes using UV irradiation is described. 12,12‐Ammonium ionenes were functionalized with either cinnamate or styrenic functionality at the chain ends and were subsequently exposed to UV irradiation. In addition, a novel copolymer (poly(cinDMPC‐co‐bocDMPC)) was mixed with the cinnamate‐functionalized 12,12‐ammonium ionene to prepare photo‐cross‐linked networks. The UV irradiation process was monitored via UV–Vis spectroscopy after each exposure. The chain‐extended 12,12‐ammonium ionene possessed superior tensile properties compared to the original 12,12‐ammonium ionene. Successful cross‐linking was confirmed with solubility testing of the thin films in methanol. The results indicated that photo‐cross‐linked 12,12‐ammonium ionenes offer potential in membrane technology applications.magnified image
Confocal Raman Microspectroscopy: A Non‐Invasive Approach for in‐Depth Analyses of Polymer Substrates
Authors: Tomba J. Pablo , Pastor José M. 
Published: March 2009

AbstractWe present a simple approach that avoids the problem of coupling fluid‐sample interaction in confocal Raman depth‐profiling with immersion objectives. We use a thin protective medium (PE film) that preserves the sample from direct contact with the coupling fluid, and that can be readily attached/de‐attached from the sample surface by applying a small difference of pressure. The strategy was tested performing confocal Raman depth profiling studies on a series of transparent substrates (PP films; 25, 44 and 60 µm nominal thickness) and comparing the results with those obtained with conventional approaches. It is shown that the strategy recovers very well all the film features, preserving at the same time a good depth resolution and optical throughput.magnified image