bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biocompatibility of polyglycidol with human peripheral blood mononuclear cells
Authors: Huang Yi‐Chun , Royappa A. Timothy , Tundel Sofia , Tsukamoto Kana , Sharma Venkatanarayanan 
Published: November 2008

AbstractPolyglycidol (PGly), a hyperbranched water‐soluble polyether with numerous terminal hydroxyl groups, has structural similarities with polyethylene glycol. Our laboratory is exploring drug delivery using hyperbranched PGly‐cytokines conjugates. Using human peripheral blood mononuclear cells and a tumor derived human B cell line, HBL‐1; various PGly concentrations were tested for cytotoxicity. Our data indicated that hyperbranched PGly was similar in toxicity to PEG 8000, a commercial polymer. It was concluded that hyperbranched PGly did not exhibit significant cytotoxicity and showed promise for use as a biocompatible polymer. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009
Mining transcriptome data for function–trait relationship of hyper productivity of recombinant antibody
Authors: Charaniya Salim , Karypis George , Hu Wei‐Shou 
Published: November 2008

AbstractIn the past decade we have witnessed a drastic increase in the productivity of mammalian cell culture‐based processes. High‐producing cell lines that synthesize and secrete these therapeutics have contributed largely to the advances in process development. To elucidate the productivity trait in the context of physiological functions, the transcriptomes of several NS0 cell lines with a wide range of antibody productivity were compared. Gene set testing (GST) analysis was used to identify pathways and biological functions that are altered in high producers. Three complementary tools for GST—gene set enrichment analysis (GSEA), gene set analysis (GSA), and MAPPFinder, were used to identify groups of functionally coherent genes that are up‐ or downregulated in high producers. Major functional classes identified include those involved in protein processing and transport, such as protein modification, vesicle trafficking, and protein turnover. A significant proportion of genes involved in mitochondrial ribosomal function, cell cycle regulation, cytoskeleton‐related elements are also differentially altered in high producers. The observed correlation of these functional classes with productivity suggests that simultaneous modulation of several physiological functions is a potential route to high productivity. Biotechnol. Bioeng. 2009;102: 1654–1669. © 2008 Wiley Periodicals, Inc.
ChemInform Abstract: Tools and Tactics for the Optical Detection of Mercuric Ion
Authors: Nolan Elizabeth M. , Lippard Stephen J. 
Published: November 2008

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
High‐density sphere packing for discrete element method simulations
Authors: Labra Carlos , Oñate Eugenio 
Published: November 2008

AbstractThe first step in a discrete element simulation is the discretization of the domain into a set of particles. The cost of generating a good cylindrical or spherical packing has resulted in a great number of approaches during the last years. A new algorithm is proposed for high‐density packing using a scheme that minimizes the distance between each particle. Using the support of a finite element mesh, less time is needed in order to achieve a low porosity configuration. In addition, a boundary constraint is introduced. The application of the same optimization scheme is used as a condition to force a good surface definition. The results obtained show a high efficiency for the generation of low porosity packing, achieving values smaller than 10% in 2D cases and 30% in 3D cases. Copyright © 2008 John Wiley & Sons, Ltd.
Virtual stability: Constructing a simulation model
Authors: Voorhees Burton 
Published: November 2008

AbstractAfter a discussion of the importance of stability and instability for complex systems theory, we define the concept of virtual stability as a state in which a system employs self‐monitoring and adaptive control to maintain itself in a configuration that would otherwise be unstable. The energy expended in this gains the system an increase in its flexibility of behavioral response to environmental changes. A model designed to illustrate virtual stability is presented, followed by a brief discussion of the evolutionary advantage this capacity provides. This leads to the suggestion that such advantage gives an argument both for the directionality of evolution and for the emergence of self‐consciousness. © 2008 Wiley Periodicals, Inc. Complexity, 2009
Enhancement of attachment and cognitive development of young nursery‐reared chimpanzees in responsive versus standard care
Authors: van IJzendoorn Marinus H. , Bard Kim A. , Bakermans‐Kranenburg Marian J. , Ivan Krisztina 
Published: November 2008

AbstractForty‐six nursery‐reared chimpanzee infants (22 females and 24 males) receiving either standard care (n = 29) or responsive care (n = 17) at the Great Ape Nursery at Yerkes participated in this study. Standard care (ST) consisted primarily of peer‐rearing, with humans providing essential health‐related care. Responsive care (RC) consisted of an additional 4 hr of interaction 5 days a week with human caregivers who were specially trained to enhance species‐typical chimpanzee socio‐emotional and communicative development. At 9 months, ST and RC chimpanzees were examined with the Bayley Scales for Infant Development to assess their Mental Development Index (MDI). At 12 months, the chimpanzees were assessed with their human caregivers in the Ainsworth Strange Situation Procedure (SSP). In this first study to use the SSP in chimpanzees, nursery‐reared chimpanzees exhibited the definite patterns of distress, proximity seeking, and exploration that underpin the SSP for human infants. In ST chimpanzees the attachment classification distribution was similar to that of human infants raised in Greek or Romanian orphanages. RC chimpanzees showed less disorganized attachment to their human caregivers, had a more advanced cognitive development, and displayed less object attachment compared to ST chimpanzees. Responsive care stimulates chimpanzees' cognitive and emotional development, and is an important factor in ameliorating some of the adverse effects of institutional care. © 2008 Wiley Periodicals, Inc. Dev Psychobiol 51: 173–185, 2009
On the Immobilisation of Catechol Phosphane Palladium Complexes on Titania
Authors: Chikkali Samir , Gudat Dietrich , Mallissery Samith Komath 
Published: November 2008

AbstractTwo isomeric bis(phosphane) palladium chloride complexes with functional catechol phosphane ligands were immobilised on different specimens of TiO2. The solid materials were characterised by 31P NMR spectroscopy both when dry and as suspensions in various solvents. The NMR spectroscopic studies demonstrated that the resistance towards leaching varies strongly with the nature of the carrier material and that the surface‐bound complexes may become detached from the support in certain circumstances in the presence of solvents that are sufficiently polar or contain reactive components such as amines.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Effect of Ligand Spacer on Metallosupramolecular Architectures: From a Dinuclear Copper(II) Double Helicate to a Tetranuclear Copper(II) Complex
Authors: Halder Partha , Banerjee Priya Ranjan , Zangrando Ennio , Paine Tapan Kanti 
Published: November 2008

AbstractTwo new bis(N,O‐bidentate) Schiff base ligands with asymmetric spacers between the donor sites have been synthesized. The asymmetric ligand H2L1 self‐assembles with copper(II) ions to give rise to a dinuclear copper(II) complex [Cu2L12] (1), while a more flexible ligand H2L2, having an additional methylene group in the spacer, forms a tetranuclear copper(II) cluster [Cu4L24] (2). Both complexes have been characterized by different analytical and spectroscopic techniques. Single‐crystal X‐ray diffraction study of 1 shows a dinuclear copper(II) double‐stranded helicate with the two heterotopic ligands oriented head‐to‐head (HH), with a separation of 7.687(3) Å between the distorted tetrahedral copper(II) centers. The bis(bidentate) ligand strands wrap around the copper centers with a similar relative orientation (HH) to give rise to a double‐helical structure. The intramolecular face‐to‐face π–π interaction stabilizes the double‐helical structure of the molecule. In the tetranuclear complex 2, the four copper(II) centers, separated by 7.288(2)–8.239(2) Å, define a slightly distorted tetrahedron.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Ruthenium Complexes Containing Pyridine‐2‐carbaldehyde Azine as a Synthon in the Synthesis of Bi‐/Trimetallic Complexes
Authors: Dwivedi Sudhakar D. , Singh Ashish K. , Singh Sanjay K. , Sharma Sanjeev , Chandra Manish , Pandey Daya S. 
Published: November 2008

AbstractRuthenium complexes [(η5‐C5H5)Ru(PPh3)(κ2‐paa)]PF6 (paa = pyridine‐2‐carbaldehyde azine) and [(η5‐C5H5)Ru(κ1‐dppm)(κ2‐paa)]BF4 [dppm = bis(diphenylphosphanyl)methane] have been employed as a synthon in the synthesis of homo/hetero bi‐/trimetallic complexes. It is the uncoordinatedN,N‐donor site on paa in complex [(η5‐C5H5)Ru(PPh3)(κ2‐paa)]PF6, and N,N‐donor site on paa and pendant phosphorus in [(η5‐C5H5)Ru(κ1‐dppm)(κ2‐paa)]BF4 that allows for their incorporation into bi‐/trimetallic systems. The resulting complexes have been characterized by analytical, spectral and electrochemical studies. Molecular structures of homo‐bimetallic complex [(PPh3)(η5‐C5H5)Ru(μ‐paa)Ru(η6‐C10H14)Cl](PF6)2 and hetero‐bimetallic complex [(PPh3)(η5‐C5H5)Ru(μ‐paa)Rh(η5‐C5Me5)Cl](PF6)2 have been authenticated crystallographically. Complexes exhibit absorptions throughout the visible region and complicated electrochemical behaviour. Metal‐based RuII/RuIII oxidations in the bimetallic complexes do not vary as in the trimetallic complexes. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Preparation of New Bis(8‐aminoquinoline) Ligands and Comparison with Bis(8‐hydroxyquinoline) Ligands on Their Ability to Chelate CuII and ZnII
Authors: Deraeve Céline , Maraval Alexandrine , Vendier Laure , Faugeroux Vanessa , Pitié Marguerite , Meunier Bernard 
Published: November 2008

AbstractNew bis(8‐aminoquinoline) ligands linked on the C2 carbon atom of both quinolines have been prepared. When the linker includes a one‐atom amino group, the corresponding compound is an excellent chelator of CuII ions and forms a mononuclear species with high metal ion selectivity relative to ZnII chelation. This selectivity for CuII ions was not observed for a bis(8‐hydroxyquinoline) analogue. The chelating properties of these ligands are compared to those of the bis(8‐aminoquinoline) ligands with different linkers between their N8 nitrogen atoms. The N8‐linked bis(8‐aminoquinoline) ligands have a higher affinity for CuII when the linker includes a three‐atom unit or when the linker is linked directly through the N8 atom. Other ligands, including that with a dimethylamino linker on C2, are less efficient and seem to chelate CuII and ZnII with an affinity that is similar to that for dipicolylamine. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)
Prins Cyclization in Ionic Liquid Hydrogen Fluoride Salts: Facile and Highly Efficient Synthesis of 4‐Fluorinated Tetrahydropyrans, Thiacyclohexanes, and Piperidines
Authors: Kishi Yuichiro , Inagi Shinsuke , Fuchigami Toshio 
Published: November 2008

AbstractPrins cyclization of homoallylic alcohols with various aldehydes was investigated in ionic liquid hydrogen fluoride (HF) salts, which played roles as a reaction medium, a catalyst, and a fluorine source. The reaction afforded the corresponding 4‐fluorinated tetrahydropyrans in excellent yields with a high stereoselectivity (cis form exclusively). When benzaldehydes having a strongly electron‐donating group at the para position were used, the stereoselectivity was lost in this system. In order to apply this method to the synthesis of other 4‐fluorinated heterocyclic compounds, we also carried out thia‐Prins and aza‐Prins cyclization in HF salt and successfully obtained 4‐fluorinated thiacyclohexanes and piperidines, respectively.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Comparing the applicability of CGE‐on‐the‐chip and SDS‐PAGE for fast pre‐screening of mouse serum samples prior to proteomics analysis
Authors: Grunert Tom , Marchetti‐Deschmann Martina , Miller Ingrid , Müller Mathias , Allmaier Günter 
Published: November 2008

AbstractIn this comparative study, a set of 13 mouse sera was investigated, which had to be checked in a fast way for homogeneity prior to complex, time‐consuming and expensive proteomics analysis. SDS‐PAGE and CGE‐on‐the‐chip were compared in terms of handling, time consumption and significance of detecting differences. SDS‐PAGE was a system giving good information on the samples at first sight and discrepancies for one sample were observed immediately after staining. The time consumption of up to 20 h was rather high (separation and staining procedure with 14×14 cm gels) in SDS‐PAGE. CGE‐on‐the‐chip system exhibited differences for two samples and, most important, analysis time was reduced to only 1 h. Of importance is that the serum protein pattern obtained by both methods may not represent the same proteins. Sample preparation was equal for both analytical techniques but necessary sample amount was only half of the material in the case of CGE‐on‐the‐chip. Statistical evaluation indicated highly critical values for only one sample in SDS‐PAGE and for two samples in the CGE‐on‐the‐chip system, both in good agreement with visual observations. Three samples showed limited homogeneity with both methods. Five and six samples, respectively, were indicated as noticeable with one of the used methods.
Highly Stable Pd‐Based Catalytic Nanoarchitectures for Low Temperature Fuel Cells
Authors: Hu F. P. , Shen P. K. , Li Y. L. , Liang J. Y. , Wu J. , Bao Q. L. , Li C. M. , Wei Z. D. 
Published: November 2008

AbstractThe multiwalled carbon nanotubes (MWCNTs) are treated with hydrofluoric acid (HF) aqueous solution so as to make enlarged micropores on the nanotube walls. Normally, there are no chemical bonds between the metal nanoparticles and the support. Therefore, the large contact area between metal nanoparticles and the support would decrease the possibility of nanoparticle agglomeration or their detachment from the support. The obtained large micropores to which the meal nanoparticles are attached can efficiently achieve the above advantages. In the experiment, the catalytic activity and the stability of Pd supported on HF treated MWCNTs (Pd/MWCNTHF) catalyst are evaluated by potential cycling for ethanol oxidation. The voltammetric data suggest that the obtained enlarged micropores on the treated nanotube walls can anchor the metal nanoparticles so effectively that it can overcome the agglomeration or detachment from the surface of the MWCNT. Pd/MWCNTHF catalyst shows improved stability.
Fault‐slip analysis in the metaophiolites of the Voltri Massif: constraints for the tectonic evolution at the Alps/Apennine boundary
Authors: Federico Laura , Spagnolo Chiara , Crispini Laura , Capponi Giovanni 
Published: November 2008

AbstractThe metaophiolitic Voltri Massif underwent a complex tectono‐metamorphic evolution, resulting from subduction to collision events during the Alpine orogenic cycle, and was subsequently involved in the first stages of the Apennine Orogeny. So this is a key area to investigate the late orogenic Alpine tectonics and its time relationship with the first Apenninic deformation events.We performed detailed mapping, fault‐slip analysis and study of fault rocks in a study area located at the eastern border of the Voltri Massif. Here we recognized two main generations of reverse shear zones (RSZ). The first one (RSZ1) is associated to mylonites and protomylonites in greenschist to low‐greenschist metamorphic facies and is characterized by a ductile to ductile–brittle behaviour; RSZ1 display either top‐to‐N–NW or to‐SW kinematics. The second generation of reverse shear zones (RSZ2) is characterized by fault breccia, crush breccia or protocataclasite; RSZ2 sense of tectonic transport ranges from top‐to‐NW, ‐SW and ‐NE.RSZ1 structures are locally folded by 10 m‐scale, open chevron folds with fold axes gently plunging to SW that likely represent the last phases of deformation during Alpine collision.RSZ2 structures locally reactivate RSZ1, with a more brittle behaviour and produce a huge volume of fault rock. They are coeval with low‐greenschist to zeolite‐facies metamorphic conditions. The RSZ2 fit a transpressional regime active at the eastern border of the Voltri Massif at least during the late Oligocene–early Miocene. Copyright © 2008 John Wiley & Sons, Ltd.
High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis
Authors: Talbot Clifford B. , McGinty James , Grant David M. , McGhee Ewan J. , Owen Dylan M. , Zhang Wei , Bunney Tom D. , Munro Ian , Isherwood Beverly , Eagle Rob , Hargreaves Andre , Dunsby Chris , Neil Mark A. A. , French Paul M. W. 
Published: November 2008

AbstractWe report an automated optically sectioning fluorescence lifetime imaging (FLIM) multiwell plate reader for high content analysis (HCA) in drug discovery and accelerated research in cell biology. The system utilizes a Nipkow disc confocal microscope and performs unsupervised FLIM with autofocus, automatic setting of acquisition parameters and automated localisation of cells in the field of view. We demonstrate its applications to test dye solutions, fixed and live cells and FLIM‐FRET. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating
Authors: Schnettler Reinhard , Knöß Peter D. , Heiss Christian , Stahl Jens‐Peter , Meyer Christof , Kilian Olaf , Wenisch Sabine , Alt Volker 
Published: November 2008

AbstractThe combination of hydroxyapatite (HA) implants serving as osteoconductive scaffold with growth factors is an interesting approach for the improvement of bone defect healing. The purpose of this study was to test whether recombinant human bone morphogenetic protein‐2 (rhBMP‐2) coating of solid HA‐implants improves bone formation in a cortical bone defect. Cylindrical trephine mill defects (diameter: 9.8 mm, depth: 10 mm) were created into the cortical tibia shaft of minipigs and subsequently filled either by plain HA cylinders (Endobon®) or by rhBMP‐2‐coated HA cylinders. Fluorochrome labeling for the evaluation of time‐dependent bone formation was done on days 8, 9, and 10 postsurgery with tetracyclin‐100, at days 25 and 30 with alizarin‐komplexon, and finally on days 32, 37, 73, and 79 with calcein green. Twelve weeks after implantation, the tibiae were harvested and were prepared for standard histological staining, fluorochrome analysis, and histomorphometry. Coating of HA implants with rhBMP‐2 led to significant enhanced new bone formation of 84.7% (±4.6%) of the implant area with almost complete bony incorporation compared with only 27.7% (±8.5%) in the uncoated HA implants (p = 0.028). In both types of implants, osteoconduction of HA led to bone ingrowth of the surrounding host bone into the implants. However, only rhBMP‐2‐coated implants showed multitopic de novo bone formation reflecting the osteoinductive properties of rhBMP‐2 in all areas of the HA implant. This study showed that the coating of HA ceramic implants with rhBMP‐2 can significantly enhance new bone formation attributable to its osteoinductive effects. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Coralline hydroxyapatite is a suitable bone graft substitute in an intra‐articular goat defect model
Authors: Koëter S. , Tigchelaar S. J. , Farla P. , Driessen L. , van Kampen A. , Buma P. 
Published: November 2008

AbstractIntra‐articular defects can be filled with an autologous bone graft taken from the iliac crest. This can be indicated after trauma or following correcting osteotomy. Patients may encounter donor site morbidity after this procedure. In this in vivo study, we studied if coralline hydroxyapatite (CHA) is a suitable material to replace autologous bone graft to fill a defect in the femoral trochlea of goats. CHA did not evoke any negative reaction in the synovium, and the articular cartilage was comparable to controls. In the bone graft group, we found scattered areas of (enchondral formed) bone. Most bone graft had been resorbed or remodeled, and the scarce remnants were incorporated into new bone. Resorption of CHA was limited or absent and most CHA was surrounded by new bone. In areas with fragmented CHA, close to the joint surface, numerous giant cells were found. The study shows that in this animal model, CHA inserted in a defect that directly communicates with the joint space incorporates into bone. This study did not show any negative effects of CHA in a joint environment. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Dual functions for LTBP in lung development: LTBP‐4 independently modulates elastogenesis and TGF‐β activity
Authors: Dabovic Branka , Chen Yan , Choi Jiwon , Vassallo Melinda , Dietz Harry C. , Ramirez Francesco , von Melchner Harald , Davis Elaine C. , Rifkin Daniel B. 
Published: November 2008

AbstractThe latent TGF‐β binding proteins (LTBP) ‐1, ‐3, and ‐4 are extracellular proteins that assist in the secretion and localization of latent TGF‐β. The null mutation of LTBP‐4S in mice causes defects in the differentiation of terminal air‐sacs, fragmented elastin, and colon carcinomas. We investigated lung development from embryonic day 14.5 (E14.5) to day 7 after birth (P7) in order to determine when the defects in elastin organization initiate and to further examine the relation of TGF‐β signaling levels and air‐sac septation in Ltbp4S−/− lungs. We found that defects in elastogenesis are visible as early as E14.5 and are maintained in the alveolar walls, in blood vessel media, and subjacent airway epithelium. The air‐sac septation defect was associated with excessive TGF‐β signaling and was reversed by lowering TGF‐β2 levels. Thus, the phenotype is not directly reflective of a change in TGF‐β1, the only TGF‐β isoform known to complex with LTBP‐4. Reversal of the air‐sac septation defect was not associated with normalization of the elastogenesis indicating two separate functions of LTBP‐4 as a regulator of elastic fiber assembly and TGF‐β levels in lungs. J. Cell. Physiol. 219: 14–22, 2009. © 2008 Wiley‐Liss, Inc.