bluuebunny/crossref_metadata_2025_split huggingface dataset:
Double‐blind crossover trial comparing beclomethasone dipropionate and sodium cromoglycate in perennial allergic rhinitis
Authors: TANDON M. K. , STRAHAN E. G. 
Published: April 2006

SummaryA double‐blind crossover trial comparing the clinical efficacy of intranasal beclomethasone dipropionate and intranasal sodium cromoglycate was carried out in fourteen patients with perennial rhinitis due to animal danders. Intranasal beclomethasone dipropionate was significantly more effective than intranasal sodium cromoglycate in relieving nasal obstruction, nasal discharge and sneezing. Eleven patients reported preference for beclomethasone dipropionate and three had no preference for either drug.
The isolation of an allergen from extracts of Dermatophagoides pteronyssinus using a murine IgA myeloma with a specificity for phosphorylcholine
Authors: STEWART G. A. , McWILLIAM A. , TURNER K. J. 
Published: April 2006

SummaryThe presence of a high molecular weight glycoprotein component bearing the hapten phosphorylcholine (Pc) has been demonstrated in some but not all extracts of house dust mite using a phosphorylcholine specific IgA myeloma protein (S107). The Pc bearing component was isolated from house dust mite extracts by precipitation at equivalence using whole myeloma serum and the isolated fraction was found to be allergenic as judged by a direct RAST assay using a highly house dust mite allergic human serum pool. RAST inhibition studies using free hapten and direct RAST studies using pneumococcal polysaccharide C‐substance indicated that the Pe moiety did not contribute significantly to the immunodominant portion of the isolated allergen. Further studies indicated that the S107 isolated material was immunochemically different from the tridacnin reactive material and the con A reactive material previously isolated from extracts of house dust mite.
A critical evaluation of RAST to venoms of Hymenoptera
Authors: SETTIPANE G. A. , CARLISLE C. C. 
Published: April 2006

SummaryRAST to venoms were done on 108 sera. Positive RAST to one or more venoms of Hymenoptera found in 51% (41/80) patients with a generalized reaction to the sting of Hymenoptera and in 7% (2/28) of normal controls were critically evaluated. RAST determinations for each sera were done in duplicate and results averaged. The laboratories were not told which sera belonged to patients with allergy to Hymenoptera and which belonged to controls. In ten patients, one half of the original sera were saved and RAST to venoms were repeated approximately 1 years later. Of our positive RASTs, 49% (20/41) had positives to more than one type of venom. The most frequent positive RAST was to yellow jacket followed by hornet, wasp, and honey bee. A high degree of cross‐reactivity occurred between venoms of hornet, wasp, and yellow jacket. The frequency of positive RAST was similar in pre‐treatment and post‐treatment sera from patients on immunotherapy with whole body extract. Specific IgE (RAST) results to venoms did not correlate with the degree of clinical severity of the sting. However, IgG anti‐venom antibodies were not considered. Elevated RAST titres were associated with increased total IgE levels in sera of non‐atopic patients. A positive RAST to venoms is frequently found in sera of patients stung within 5 years. RAST scores of 2 to 4 were remarkably reproducible, while scores of 1 were not consistently reproducible. RAST results should be interpreted in conjunction with the clinical history.
Management of chronic idiopathic urticaria by the identification and exclusion of dietary factors
Authors: GIBSON ANNE , CLANCY R. 
Published: April 2006

SummaryThe role played by dietary chemical factors in the pathogenesis of chronic idiopathic urticaria (CIU) was assessed in seventy‐six patients by challenge. Stable remission was first established by using an empirically established ‘exclusion diet’. A diet modified to exclude those chemicals giving a positive response to challenge was demonstrated to be of therapeutic value for time periods of up to 18 months. Re‐testing twelve patients at 12 months indicated that most patients positive to salicylate or benzoate challenge retained this pattern of reactivity.
Potentiation by polymyxin B of the bronchoconstriction following allergen inhalation in patients with atopic asthma
Authors: ROZNIECKI J. , GORSKI P. , POLANSKA Z. 
Published: April 2006

SummaryThe effect of a single inhalation of allergen on bronchial reactivity to polymyxin B was studied in eight patients with hay fever. In winter the reaction to polymyxin B before and after inhalation of pollen allergen is measured by the FEV. In all patients bronchial reactivity to polymyxin B increased under the influence of allergen.
Cutaneous basophil hypersensitivity in atopic dermatitis
Authors: HÉNOCQ E. , GAILLARD J. 
Published: April 2006

SummaryThe infiltration by basophils into delayed hypersensitivity skin lest sites was examined in patients with atopic dermatitis, contact dermatitis and in normal healthy persons.Atopic dermatitis patients, with large amounts of IgE, injected intradermally with staphylococcal antigens showed reactions that were more transient and erythemaious than those of normal persons. On hislological examination there were numerous, degranulating basophils among the perivascular mononuclear cells. Normal persons, with small amounts of IgE showed typical mononuclear cell infiltration and few basophils. Patients with contact dermatitis, one with much IgE, responded to patch tests to potassium dichromate or to nickel sulphate by delayed type reactions, but on histology, two of the four patients showed a significant infiltration by basophils.It is considered that the erythematous response to the antigen in atopic dermatitis patients is related to the basophil infiltration.
Flow‐cytometric analysis of human basophil degranulation
Authors: NAKAGAWA T. , STADLER B. M. , HEINER D. C. , SKVARIL F. , DE WECK A. L. 
Published: April 2006

SummaryQuantification of human basophil degranulation induced by anti‐IgE, anti‐IgG4, and by ionophore was performed using a flow‐cytometric system. It was shown that these antibodies and ionophore can degranulate basophils in a dose‐dependent manner, and that there is a wide variation in the response of basophils obtained from different individuals to these stimuli. A significant correlation was observed between the degree of degranulation induced by anti‐IgE and anti‐IgG4, while this was not the case for anti‐IgE and ionophore. It was also shown that IgG4 myeloma protein can passively sensitize basophils. In general, degranulating efficacy was in the order of ionophore > anti‐IgE >anti‐IgG4, both in allergic and non‐allergic individuals.
Differences in response to anti‐IgE and to anti‐IgG in basophils from patients with bronchial asthma
Authors: KIMURA I. , TANIZAKI U. , SATO S. , TAKAHASHI K. 
Published: April 2006

SummaryPeripheral blood basophils of thirty‐eight patients with bronchial asthma were examined for their reactivity to anti‐IgE and anti‐IgG antisera. Basophils of patients with serum IgE levels of more than 1001 i.u./ml reacted strongly to anti‐IgE. Basophils of patients with serum IgE levels of less than 100 i.u./ml had a tendency to react more strongly to anti‐IgG. An index (basophil ratio) was devised to compare the patient basophil reactivity to anti‐IgE and anti‐IgG. This basophil ratio was lower (IgE dominant) in the atopic cases which usually exhibited a high serum IgE level. Most cases with low serum IgE exhibited a high basophil ratio (IgG dominant). The basophils of seven intractable patients reacted more strongly to anti‐IgG than to anti‐IgE regardless of the serum IgE level.
Supravital observation of in vitro basophils in immunological reactions
Authors: KIMURA I. , TANIZAKI Y. , SATO S. , TAKAHASHI K. , SAITO K. , UEDA N. 
Published: April 2006

SummaryThe migration velocity and morphology of basophils in vitro were examined after the addition of anti‐immunoglobulin (anti‐lgE or anti‐IgG). In atopic asthma patients with high serum IgE levels (more than 1000 i.u./ml) basophils showed increased migration velocity and showed pear‐shaped and palmate processes after the addition of anti‐lgE, but on the addition of anti‐IgG, these basophils did not show increased migration velocity or morphological changes. In intractable asthma patients with low serum IgE levels, these changes occurred with anti‐IgG but not with anti‐IgE. These in vitro findings suggest a basophil reactivity to anti‐IgE and anti‐IgG in individual patients and support our previous differentiation of asthma patients according to basophil reaction.
Type I allergy in mushroom soup processors
Authors: SYMINGTON* I. S. , KERR J. W. , McLEAN D. A. 
Published: April 2006

SummaryEight workers employed in a food manufacturing factory presented with symptoms of rhinorrhoea, dyspnoea and wheezing, originating during the preparation of dried mushroom soup. Five had positive immediate skin tests to dried mushroom extract and in four the FEV1 fell by over 30% following a bronchial challenge with mushroom dust. The features detected were those of a type I allergic response, without evidence of the extrinsic allergic alveolitis described in ‘mushroom worker's lung’.
Miller moth asthma
Authors: STORMS W. W. , BERRY C. , WITHEE W. 
Published: April 2006

SummaryThe characteristics of the allergen producing asthma caused by inhaled miller moth particles were investigated by skin testing and by Sephadex fractionation. No major difference in skin test reactivity was found between the wings and the bodies of the moths. Two fractions of the moth extracts were found to contain allergic reactivity. The importance of detailed history‐taking in order to discover such causes of asthma is stressed.
Complement consumption in eggwhite and fish sensitivity
Authors: BERRENS L. , VAN DIJK A. G. , WEEMAES C. M. R. 
Published: April 2006

SummaryA case of eggwhite allergy and a case offish allergy, where complement‐fixation by thermostable IgG‐class antibody was positive at extremely high antigen dilutions are described. The antibody in eggwhite allergy was of the precipitating variety and the C‐fixation phenomenon was not related to the presence or absence of IgE antibody.
The effect of complement depletion on hypersensitivity pneumonitis lesions induced by Micropolyspora faeni antigen
Authors: WILSON M. R. , SCHUYLER M. R. , CASHNER F. , SALVAGGIO J. E. 
Published: April 2006

SummaryAnimals sensitized by intratracheal administration of particulate Micropolyspora faeni antigen and subsequently challenged with the antigen intratracheally developed lesions of hypersensitivity pneumonitis histologically similar to those observed in man with this disease. Animals sensitized with antigen but depleted of complement with cobra venom factor prior to challenge with the antigen manifested a significant reduction in mean lesion indices when compared to a group of control animals that were not complement‐depleted. These data indicate that complement is necessary for the development of pulmonary lesions of experimental hypersensitivity pneumonitis in the rabbit.
Eczema and infant diet
Authors: KERGUSSON D. M. , HORWOOD L. J. , BEAUTRAIS A. L. , SHANNON F. T. , TAYLOR B. 
Published: April 2006

SummaryThe relationship between parental atopy, breast‐feeding, early solid food diet and the rate of eczema was studied in a birth cohort of 2‐year‐old children. Rates of eczema varied significantly with parental atopy and solid feeding: children of atopic parents given solid food during the first 4 months had over two‐and‐a‐half times the rate of eczema of children not given solid food and who had non‐atopic parents. Further, rates of eczema increased in almost direct proportion to the number of different types of solid food that the child had been given during the first 4 months. Breast‐feeding had no significant effect on rates of eczema.The results suggest that both parental atopy and diversity in early diet are factors which contribuie towards rates of childhood eczema.
Analysis of green coffee bean and castor bean allergens using RAST inhibition
Authors: LEHRER S. B. , KARR R. M. , SALVAGGIO J. E. 
Published: April 2006

SummaryCoffee workers with occupational allergic symptoms and positive skin tests to green coffee bean and factor dust antigens have elevated serum IgE antibodies (by radioallergosorbent test–RAST) to green coffee and castor bean allergens. These antibodies were used in a RAST inhibition assay to analyse coffee and castor allergens. Bean allergens were extracted by homogenization in PBS, centrifugation and concentration of supernates by ultrafiltration. Green coffee bean allergens, fractionated by gel filtration and Pevikon block electrophoresis, were shown to be very heterogeneous with a molecular weight range of 50 000 to 500 000 daltons. Castor allergens were more homogeneous with a molecular weight of 14 000 daltons and were partially purified by Pevikon block electrophoresis, gel filtration and isoelectrofocusing. Chemical analysis showed that protein was the major component in both allergen extracts. However, proteolytic enzymes could only partially destroy allergenic activity. Such isolation and characterization of these allergens should result in better methods of diagnosis and treatment of coffee workers with occupational allergic disease.
A double‐blind trial comparing disodium cromoglycate (DSCG), and ketotifen in extrinsic asthmatic children
Authors: SCHUHL J. F. , DE CUESTA D. HOLGADO 
Published: April 2006

SummaryA double‐blind non crossover trial comparing disodium cromoglycate (DSCG), with ketotifen, was carried out in twenty‐seven extrinsic asthmatic children over 12 weeks. Assessment was made using symptom score and drug score charts. Both drugs showed a similar protective effect in the group studied. None of the subjects had to withdraw from the trial and no side‐effects were noted in weight‐curve, arterial pressure and pulse rate. PEFR values remained unchanged for both drugs throughout the trial. A non‐significant tendency for additional corticosteroid support in the Ketotifen group was noted.
A controlled trial of oxatomide in the treatment of asthma with or without perennial rhinitis
Authors:   BROMPTON HOSPITAL/MEDICAL RESEARCH COUNCIL COLLABORATIVE TRIAL
Published: April 2006

SummaryA double‐blind study comparing oxatomide, a new oral drug with anti‐allergic properties, with placebo in the treatment of patients with asthma with or without perennial rhinitis has been undertaken. Sixty‐two patients were followed up to 28 weeks and fifty‐two to 52 weeks. On admission to the study the great majority of the patients were receiving inhaled steroids and about a third sodium cromoglycate for their asthma. The results of the study suggest that oxatomide has some activity but have failed to show that it is substantially better than placebo in this group of patients. For the first time weight‐gain is reported as a side effect of oxatomide.
Lymphocytes with receptors for IgG and IgM in atopic eczema and their relationship to serum IgE levels
Authors: CAMPBELL M. A. 
Published: April 2006

SummaryTwenty patients with atopic eczema were compared with twenty non‐atopic healthy controls. The atopic patients had significantly reduced percentages of lymphocytes bearing receptors for the Fc fragment of IgG (EAγ) in the peripheral blood. Percentages of lymphocytes with receptors for the Fc fragment of IgM (EAγ) did not differ significantly between the atopics and the controls. Serum IgE levels were significantly raised in the patient group and overall serum IgE levels were significantly inversely correlated with percentage EAγ. Although significant (P=0.007), this correlation was not strong (r=0.43) and the relevance of this to atopic disease is discussed.
Serum and salivary immunoglobulin A and free secretory component in ulcerative colitis
Authors: MORRIS T. J. , MATTHEWS N. , RHODES J. 
Published: April 2006

SummaryPatients with ulcerative colitis have been investigated for evidence of defects in secretory immunity. Total serum IgA concentration, serum IgA antibody titres to Candida albicans, salivary IgA and free secretory component concentrations have been measured in thirty‐six patients with ulcerative colitis and thirty‐six normal controls. None of the parameters was significantly different between patients with proctitis and patients with extensive colitis or between the colitis patients and normal controls.
Bronchial asthma due to inhaled wood dust: Tanganyika aningré
Authors: PAGGIARO P. L. , CANTALUPI R. , FILILRI M. , LOI A. M. , PARLANTI A. , TOMA G. , BASCHIERI L. 
Published: April 2006

SummaryThree cases of bronchial asthma due to wood dust from Tanganyika anigré are reported. All patients were woodworkers and had symptoms of dyspnoea, cough and wheezing, and sometimes itchiness and rhinorrhoea, after exposure to Tanganyika aningré. The intradermal skin tests were positive for untigenic extract of Tanganyika aningré. In two patients the bronchial provocation test with the wood dust and with the soluble extract inhaled by aerosol was positive showing an immediate reaction; in the same patients bronchial hyperreactivity was also found. Atopy was present in one subject. No precipitins or specific IgE were found in patient sera by the immunodiffusion technique or by RAST.
Increased plasma histamine levels in migraine patients
Authors: HEATLEY R. V. , DENBURG J. A. , BAYER N. , BIENENSTOCK J. 
Published: April 2006

SummaryWhole blood and plasma histamine levels, peripheral basophil and eosinophil counts and serum immunoglobulins have been measured in a group of eighteen patients with migraine in remission and in twelve of these patients during a headache attack. Plasma histamine levels were significantly elevated (P < 0.0005) in patients with migraine both during headache attacks and symptom‐free periods.
Antibody against a pigeon bloom‐extract: a further antigen in pigeon fanciers' lung
Authors: BANHAM S. W. , McKENZIE H. , McSHARRY C. , LYNCH P. P. , BOYD G. 
Published: April 2006

SummaryPigeon bloom, a waxy powder which coats the feathers of racing pigeons, has been investigated as a potential antigen in pigeon fanciers' lung. Precipitins to a soluble bloom‐extract were demonstrated in fifty‐nine of ninety‐seven people studied at two Scottish pigeon shows, whilst precipitins to pigeon serum were found in thirty‐seven. A radioimmunoassay was developed to allow an estimation of the intensity of antibody response to this antigen in nineteen pigeon fanciers being investigated for pigeon fanciers' lung. The mean antibody level to bloom extract was significantly higher (P>0.05) in those with acute symptoms compared to those with less severe symptoms. Pigeon bloom is a highly antigenic material and provides an alternative to pigeon droppings as a source for the inhalant antigens regularly encountered by those keeping pigeons.
Comparison of sodium cromoglycate pressurized aerosol and powder in the treatment of asthma
Authors: LAL S. , MALHOTRA S. M. , GRIBBEN M. D. 
Published: April 2006

SummaryThe effect of a pressurized aerosol formulation of sodium cromoglycate divided in doses of 8 mg a day was compared with that of a standard sodium cromoglycate powder (80 mg a day) in a double blind crossover trial lasting 16 weeks. No significant differences between the two formulations were detected in terms of patients' asthma severity and lung function, recorded monthly at the clinic and daily by the patients. The pressurized aerosol in a much smaller dose appears to be as effective a method as standard cromoglycate, and an alternative method of inhaling sodium cromoglycate for patients who prefer an aerosol to a dry powder formulation.
Allergen‐specific IgE and IgG4 antibody levels in sera and the capacity of these sera to sensitize basophil leucocytes in vitro
Authors: van TOORENENBERGEN A. W. , AALBERSE R. C. 
Published: April 2006

SummaryLeucocytes from normal non‐allergic donors were passively sensitized with sixty‐five atopic sera that contained IgE directed against house dust mite (HDM) or guinea‐pig dander (GPD). In no serum was there any IgE with an abnormal basophil anaphylactic‐sensitizing activity.In sera from patients hypersensitive to GPD, both GPD‐specific IgE and IgG4 were measured. A significant correlation was found between the GPD‐IgE RAST and the basophil‐sensitizing capacity of GPD‐positive sera, but no correlation was found between this sensitizing capacity and the GPD‐lgG4 RAST.Leucocytes were passively sensitized with serum mixtures that contained the same amounts of total and HDM‐specific IgE; these mixtures differed as to sensitizing activity. It is unclear whether this variation is due to differences in IgE or to influences of other factors in serum.
Clinical associations with serum allergen‐specific IgG4 antibodies
Authors: GWYNN C. M. , ALMOSAWI T. , STANWORTH D. R. 
Published: April 2006

SummarySerum total and allergen‐specific IgE and IgG4 antibodies, were measured in eighty‐seven atopic and nineteen non‐atopic asthmatics. The allergens studied were: Dermatophagoides pteronyssinus, grass pollens, cat dander, dog dander, milk and egg. Sixty‐eight atopic asthmatics and fourteen non‐atopic asthmatics were found to have allergen‐specific IgG4 antibodies, to at least one of the allergens tested. IgG4 antibodies to milk and egg were common in both groups of asthmatics, and to animal danders in the non‐atopic asthmatics.Skin prick tests were always negative when allergen‐specific IgG4 antibodies occurred alone, but in such cases, intradermal skin tests were positive.Seventy‐five per cent ofa group of patients with normal levels of serum total IgG4, were found to have at least one positive IgG4 RAST.
The effect of disodium cromoglycate on rectal mucosal histamine release, eosinophil exudation and disease activity in active ulcerative colitis
Authors: RAMPTON D. S. , BROWN M. J. , CAUSON R. , SAHIB M. 
Published: April 2006

SummaryDisodium cromoglycate has been reported to benefit some patients with active ulcerative colitis. We have investigated the effect of cromoglycate in high dose on rectal mucosal mast cell degranulation (assessed indirectly by measurement of mucosal histamine release, using in vivo rectal dialysis, and the eosinophil content of the rectal inflammatory exudate) and on disease activity in eight patients in relapse. Although cromoglycate (1600 mg by mouth daily for 2 weeks) did not affect histamine release, it reduced (P < 0.05) the eosinophil content of the exudate towards normal. Stool frequency also fell (P < 0.05) but other aspects of bowel habit, sigmoidoscopic appearance, rectal mucosal potential difference and electrolyte transport were unaltered. Pre‐treatment values of histamine release and eosinophil exudation could not be used to predict the response to cromoglycate. The reduction in eosinophil exudation produced by the drug could be due to inhibition of mast cell release of chemotactic factors other than histamine; the therapeutic effect of cromoglycate was unimpressive.