bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the Effects of a Ketogenic Diet Full-Cycle Management Platform in Children with Refractory Epilepsy
Authors: Wang Xingyanan , Ye Jinghua , Tian Xiaoqin , Wang Fangping 
Published: April 2024

Abstract

Objective:
To develop and explore the application effects of a Ketogenic Diet Full-Cycle Management Platform in the nursing care of children with refractory epilepsy undergoing a ketogenic diet.
Methods:
A prospective, non-synchronous control study was conducted. Thirty children undergoing a ketogenic diet from August to September 2023 were selected as the control group, while thirty children undergoing the diet from October to November 2023 served as the experimental group. The control group received standard nursing care, whereas the experimental group received interventions through the Ketogenic Diet Full-Cycle Management Platform, on top of the control group's regimen. The study compared the average daily seizure frequency, the average daily incidence of adverse reactions, and the average duration of the ketogenic diet between the two groups after four weeks.
Results:
The experimental group showed a decrease in seizure frequency and adverse reactions, along with an increased average duration of the ketogenic diet compared to the control group. These findings were statistically significant (P < 0.05).
Conclusion:
The Ketogenic Diet Full-Cycle Management Platform enables parents of affected children to more conveniently and quickly grasp the knowledge related to the ketogenic diet. Medical staff can timely understand changes in the children's diet through the platform, make adjustments as needed, and thus improve the quality and effectiveness of the ketogenic diet for children with refractory epilepsy.
Research on a Coal Seam Modeling Construction Method Based on Improved Kriging Interpolation
Authors: ZHANG Jing , LIU Guangwei 
Published: April 2024

Abstract
To make the Kriging interpolation more consistent with the actual coal seam shape, combine interpolation points into a triangular mesh, extract a large triangular mesh using MATLAB for local interpolation, then identify triangular meshes with similar normal vectors. Formulate them into a plane, remove interpolation points inside the plane. Finally, continue interpolation at the boundaries, generate a triangular mesh, clip using the original boundary lines to retain internal triangular meshes, re-establish the boundary lines, and ultimately disperse the boundary lines into points. The research results show that applying this method to the construction of the Dananhu No. 2 open-pit mine coal seam model has improved the problem of coal seam transition stiffness, such as abnormal large-area triangulation in areas with large elevation differences, saving about 27000KB, about the memory occupied by the four-layer coal seam model, preventing boundary line positioning due to reasons such as too small density of points on the coal seam reserve boundary line and transitions are inaccurate, making the model transition at the boundary smoother and more consistent with the actual coal seam change trend. This method can be extended to the construction of all mine models.
Properties of postbiotics produced by probiotics: antimicrobial, antioxidant activities and production of vitamins, organic acids
Authors: Ibrahim Gamal A. , Mabrouk Ahmed M. , El-Ssayad Mohamed F. , Mehaya Fathy. M. S. , Sharaf Osama M. , Ibrahim Mohamed I. M. 
Published: April 2024

Abstract

The current study was conducted to determine and investigate the antimicrobial, antioxidant activity, vitamins, organic acid and short-chain fatty acid composition of postbiotics produced by some probiotics. In this study, the antimicrobial activity of all postbiotics from tested probiotic strains showed zones of inhibition against most indicator strains, but with different diameters. The results show that L. acidophilus and L. helveticus had the largest inhibitory effects on pathogenic bacteria and fungi. The results also show that the postbiotics have antioxidant activity. It was observed that the L. plantarum strain had the highest antioxidant activity, followed by L. rhamnosus GG and B. bifidum. The results indicated that postbiotics produced by various strains contain many organic acids in different concentrations. In conclusion, different compounds detected in the postbiotics of the probiotic strains used in this study are in preparation for the next research on their nutritional evaluation to identify the best strains for application in functional dairy products.
Cohesive transcriptomic and in vitro approach revealed genes involved in inhibition of G0/G1, G2 phase of cell cycle pathway of stomach cancer cells by geraniol treatment
Authors: Perumalsamy Haribalan , Rahimi Shadi , Gokulanathan Anandapadmanaban , Jyothiraditya Vuluchala , Dhandapani Sanjeevram , Almoajel Alia , Subramanian Sivakumar Allur , Elsadek Mohamed Farouk , Balusamy Sri Renukadevi 
Published: April 2024

Abstract

The effects of geraniol on cell cycle related pathway in AGS using RNA sequencing have not been explored and it is largely unknown. In this study, we isolated geraniol from Cymbopogon martini (palmarosa) essential oil using various spectroscopic analyses. At first, we carried out the cytotoxicity of geraniol on AGS cells. In-depth RNA sequencing analysis showed that geraniol negatively regulated genes that specifically initiate double-strand break repair via DNA replication, mitotic G1, G2/M transition, and S phases in cell cycle, eventually leading to induce apoptosis. Additionally, we validated the interaction of geraniol with the cell cycle related genes using docking, Florescence-activated cell sorting (FACS) and quantitative polymerase chain reaction (qPCR) analysis. Overall, the present investigation shows that geraniol interacts with specific target genes involved in the cell cycle process and induce cell death in the stomach cancer cells, which can be suggested as the potential treatment for stomach cancer.
Adverse Childhood Experiences, School Bullying Retrospection, and the Impact on Resilience in Adulthood
Authors: Fountain Raisa , Beckstein Amoneeta 
Published: April 2024

Abstract

Adverse childhood experiences (ACEs) have been repeatedly shown to profoundly impact individual resilience in adulthood. ACEs and bullying experiences are linked with school disengagement, depression, anxiety disorders, and substance abuse. Moreover, ACEs are associated with lower life expectancy, increased risk of heart disease, and cancer. Experiencing bullying victimization had been shown to lead to lower educational attainment, unemployment, reduced income, and poorer general health and cognitive functioning in early to midlife. Yet, there remains a gap in understanding how ACEs and bullying interact to influence resilience in adulthood. This cross-sectional study aims to explore the compounded effects of ACEs and bullying on resilience scores. The study surveyed 255 Amazon MTurk workers selected by CloudResearch under 40 years old from the United States, assessing ACEs, bullying, and resilience. Surprisingly, mild to moderate experiences of past bullying were positively linked to resilience scores, counter to expectations. However, ACEs showed no direct relationship with resilience scores. These findings prompt further investigation, considering potential sample biases from using an MTurk population, and highlight implications for future research and practice.
Integrated analysis of disulfidptosis-related genes SLC7A11, SLC3A2, RPN1 and NCKAP1 across cancers Running title: Disulfidptosis-related genes across cancers
Authors: Zheng Zequn , Song Yongfei 
Published: April 2024

Abstract

Disulfidptosis, a newly identified form of regulated cell death associated with disruption of disulfide bond formation in the endoplasmic reticulum, involves the dysregulation of disulfidptosis-related genes (DRGs) that may contribute to cancer development and progression. However, the molecular mechanisms and clinical implications of DRGs in different cancer types remain poorly characterized. Therefore, in this comprehensive study, we investigated the expression, prognostic value, and functional roles of four recently identified DRGs (SLC7A11, SLC3A2, RPN1, and NCKAP1) across various cancers. Our results demonstrated that these DRGs were significantly upregulated in multiple tumor types and were associated with poor survival outcomes. Through mutation landscape analysis, we identified diverse missense mutations in these DRGs, with NCKAP1 exhibiting the highest mutation frequency (5.9% in skin cutaneous melanoma). Additionally, we observed positive correlations between these DRGs and tumor stemness (DNAss and RNAss) as well as RNA modifications, particularly m6A modification, in several cancer types. Furthermore, high expression of SLC7A11, RPN1, and NCKAP1 was positively associated with infiltration of T-helper type 2 (Th2) cells in various cancers, while high expression of SLC7A11, SLC3A2, and RPN1 correlated with tumor mutational burden (TMB) in 10, 4, and 8 tumor types, respectively. Utilizing a protein-protein interaction network, we identified the RHO GTPases Activate WASPs and WAVEs pathway as significantly enriched, suggesting the involvement of these DRGs in cancer-related signaling pathways. Collectively, our findings provide novel insights into the molecular mechanisms and clinical implications of DRGs in pan-cancer, highlighting their potential as biomarkers and therapeutic targets for cancer treatment.
Process Optimization of Vacuum Concentration of Karonda Fruit Juice Using Response Surface Methodology: Effects on Antioxidant Activity, Iron Content and Gcms Profile
Authors: S. Chinmayi A. , J. Suresha G. , X. Rosemary M. , K. Sadananda G. , R. Venugopalan , R. Vasudeva K. , B. Suresha K. , K Swamy G. S. 
Published: April 2024

Abstract
In the realm of fruit processing, karonda juice stands out for its elevated moisture levels, posing challenges in storage and transportation. This study presents a pioneer effort for concentration of karonda juice through optimizing the process parameters viz., temperature and time using central composite design (CCD) of response surface methodology (RSM) within rotary evaporator setup. Through ten experimental runs, variations were introduced, adjusting temperatures from 45 to 55°C and duration from 90 to 210 minutes. The results showcased the efficacy of vacuum concentration, reducing moisture content to 16.43% and significantly elevating total soluble solids (TSS) from 9.02 to 89˚B. Moreover, key nutrients experienced substantial increase: ascorbic acid surged from 3.79 to 14.66 mg per 100g, total phenolic content (TPC) soared from 100.74 to 386.97 mg GAE per 100g, total antioxidant activity (TAA) (FRAP) escalated from 76.74 to 328.10 mg AAE per 100g, anthocyanin content increased from 10.9 to 129.34 mg per 100g, and iron content rose from 39 to 150.54 mg per 100g. GC-MS profile elucidated compounds like Tetrahydrofuran, 3-Hydroxy-3-methylvaleric acid, 3-Hexen-2-one, Hydroperoxide and Octane. The optimization process, guided by RSM revealed the ideal parameters: 55°C for 150 minutes, marking a significant advancement in karonda concentration techniques.
Transcriptomic and Proteomic Spatial Profiling of Pediatric and Adult Diffuse Midline Glioma H3 K27-Altered, Reveals Region Specific Differences and Limited Overlap between mRNA and Protein
Authors: Damodharan Sudarshawn , Shireman Jack M. , Xie Elliot , Distler Emily , Kendziorski Christina , Dey Mahua 
Published: April 2024

Abstract

Diffuse midline glioma, H3 K27-altered (DMG-Alt) are highly aggressive malignancies of the central nervous system (CNS) that primarily affect the pediatric population. Large scale spatial transcriptomic studies have implicated that tumor microenvironmental landscape plays an important role in determining the phenotypic differences in tumor presentation and clinical course, however, data connecting overall transcriptomic changes to the protein level is lacking. The NanoString GeoMx™ Digital Spatial Profiler platform was used to determine the spatial transcriptomic and proteomic landscape in a cohort of both pediatric and adult H3 K27-altered DMG biopsy samples. Three fluorescently labeled antibodies targeting immune cells (CD45), epithelial cells (PanCK), tumor cells (H3 K27M) and a nucleic acid stain (SYTO-13) were used to establish regions of interest (ROI) for genomic and proteomic analysis. We found genetic alterations within the tumor which can be delineated across patient age and spatial location. We show that the H3 K27M mutation itself has a profound impact on tumor cells transcriptomics and interestingly we found limited fidelity between overall transcriptome and proteome. Our data also validate the previously described OPC like precursor signature at the proteomic level and reveal a special shift in the signature based on the local TME composition.
Spirulina supplementation and circuit resistance training (CRT) reduce serum Asprosin and appetite and improve energy balance in overweight and obese Men
Authors: Nakhaei Hossein , Mogharnasi Mehdi , Dehghani Karim , Mohammadi Mahdi , Ghasemi Elham 
Published: April 2024

Abstract

Purpose
The present study aimed to investigate the effects of eight weeks spirulina supplementation and circuit resistance training (CRT) on Asprosin, appetite and energy balance of overweight and obese men.
Methods
The present study is a single-blind randomized controlled trial. Sixty overweight and obese men (BMI > 25) were purposefully selected and randomly divided into equal four groups of training plus spirulina, training plus placebo, spirulina, and placebo. The participants of the training groups performed 12 movements with 40–90 percent maximal repetition (3 sessions per week) and supplemental groups consumed 1000 mg of spirulina per day for eight weeks. Asprosin, appetite with visual analogue scales, calorie intake, energy expenditure and mass of body fat (MBF) were measured before and after intervention. To analyze data, one-way variance, paired- t-tests and Pearson correlation test was used by SPSS (version 20) at a significance level of p ≤ 0.05.
Results
After intervention Asprosin level, weight, MBF, calorie intake, and hunger declined and fullness and satiety increased in training plus spirulina, training plus placebo, and spirulina groups (p < 0.05). In addition, energy expenditure increased only in the training groups (P = 0.01). The mean changes of the research indicators in the training plus spirulina group were significantly more than the other groups (p < 0.05).
Conclusion
It seems that eight weeks of CRT and spirulina supplementation decrease the level of Asprosin and improve appetite and energy balance in overweight and obese people.
Well-being amid (im)mobility struggles: Youth’s experiences in Casamance, Senegal
Authors: Debray Alix , Petit Véronique , Ruyssen Ilse , Sow Ndiémé , Toma Sorana 
Published: April 2024

Abstract

Background Large numbers of young people worldwide, especially in the Global South, wish to migrate but lack the capacity to do so, with potentially detrimental consequences for their well-being and mental health. Termed ‘involuntary immobility’, this phenomenon is numerically larger than migration, but remains for now a largely underexplored area of research. Focusing on young Senegalese living in Casamance, this paper contributes to the limited literature on the implications of immobility for subjective well-being. It does so by (i) considering different degrees and types of involuntary immobility and their intersections, (ii) laying out the implications of (interacting) social and spatial immobilities for well-being with particular attention to youth’s agency in navigating a lack of capabilities, and (iii) by accounting for heterogenous experiences taking a gendered approach.
Methods For this study, 35 semi-structured interviews were conducted with 18- to 39-year-olds in Ziguinchor region, recruited through purposive and snowball sampling. Thematic analysis was employed on verbatim transcriptions of audio-recorded interviews.
Results Our study reveals a nuanced reality where aspirations to (temporarily) move abroad coexist with aspirations to stay in Casamance. Participants’ life aspirations and overarching projects clash, however, both with a lack of capabilities to move abroad and to enact these locally. This pervasive immobility decreases life satisfaction and generates negative emotions, such as stress, anxiety, discouragement, and distress. Despite these obstacles, our findings also underscore the agency and resourcefulness displayed by the youth as they navigate their limited control over life choices and paths.
Conclusions Involuntary spatial immobility exacerbates the dominant experience of social immobility, magnifying its effect on youth’s well-being, revealing a previously unacknowledged phenomenon. Our findings further emphasize the pressing need for a more cohesive alignment between migration policies and information campaigns on one hand and the real experiences and challenges encountered by their intended audience on the other.
Role of Melatonin-Related Signatures in the Prognosis of Glioblastoma
Authors: ma zhen , Cheng Houxiang , Shun Chao , Zhou Jinhuan , Yuan Guoqiang , Pan Yawen 
Published: April 2024

Abstract

Background
Melatonin (MT) can have a direct or indirect impact on the expression of numerous crucial genes in cancer cells. Consequently, this paper strived to investigate MT-associated prognostic genes in Glioblastoma (GBM) and their mechanisms of operation.
Methods
Based on the survival of samples in training set and the expression of 34 MT-related genes (MT-RGs), univariate Cox regression analysis and consistent cluster analysis were used to identify differential prognostic MT-RGs for follow-up analysis. Then, prognostic genes were obtained by least absolute shrinkage and selection operator (LASSO) regression analysis to construct risk model. On the basis of risk model, GBM patients in training set were sorted to high- and low-risk cohorts. Further, to predict patient survival, we combined risk score with other clinical characteristics to access independent prognostic factors for the construction of prognostic model. Moreover, immune cell infiltration analysis and drug sensitization were performed to probe the potential mechanism of prognostic genes. Additionally, single-cell analysis was utilized to recognize cell types in GBM patients, and pseudotemporal trajectories of predictive genes were predicted in key cell types.
Results
The GBM patients in the training set were divided into two clusters (Cluster 1 and Cluster 2). Based on two clusters and the expression of MT-RGs, 8 differential prognostic MT-RGs were selected by wilcoxon test. Ultimately, ECE1, CYP1B1, IRAK1, SIRT1, and ACHE were defined as prognostic genes via LASSO. Importantly, risk score and age associated with GBM prognosis were identified as independent prognostic factor to establish the prognostic model, which might be an effective tool for predicting survival in GBM patients. For immune analysis, neutrophils were markedly more abundant in high-risk cohort. And 198 drugs (e.g. Temozolomide, Cispiatin, Etoposide) were identified between two risk cohorts. Moreover, 7 cell types (Myeloid, Neoplastic, Oligodendrocytes, Astrocytes, Vcascular, OPCs, and Neurons, and Myeloid) were annotated in single-cell dataset. Pseudotemporal analysis revealed that ECE1, CYP1B1 and ACHE expression steadily decreased on Myeloid, Vascular and Neoplastic.
Conclusion
A new prognostic model for GBM associated with MT was created and verified, which might furnish new opinions for the study of MT-RGs in GBM.
Analysis of deformation and failure mechanism of sandwich beams with lattice core under three-point bending load
Authors: Taghipoor Hossein , Sefidi Mahdi 
Published: April 2024

Abstract
This paper investigates sandwich beams with lattice cores under quasi-static bending, owing to their lightweight nature and high energy absorption capabilities. Utilizing analytical methods governing beams, an investigation into their failure mechanisms is conducted, incorporating experimental and numerical results. The influence of thickness and core cell sizes on energy absorption are examined. The analysis delves into the elastic and plastic behavior of the beam, which is refined and validated against the numerical and experimental tests and failure modes of sandwich panel beams. The alignment of analytical predictions with both experimental and numerical results in terms of mean forces, and energy absorptions was remarkably precise. Moreover, evidence has been presented that the face yield and core shear failure regions are significantly impacted by variances in core dimensions. Additionally, the thickness of core cell strands was found to be pivotal in influencing the compressive and shear strengths of sandwich panel beams.
Enhancing Medical Education Through the BOPPPS (Bridge-in, Objective, Preassessment, Participatory learning, Post-assessment, Summary) : A Comprehensive Review and Analysis
Authors: Z Jing , Gan Xiaochuang , Gou Qitao , Tie Hongtao 
Published: April 2024

Abstract
BOPPPS (Bridge-in, Objective, Preassessment, Participatory learning, Post-assessment, Summary) has emerged as a compelling alternative in medical education. This study aimed to assess the efficacy of BOPPPS in medical education compared to traditional teaching strategies. Databases of PubMed, EMbase, Cochrane Library, Scopus, and Web of Science were systematically searched. The Primary outcome was the final examination score. All meta-analyses were performed by using random-effects models. Overall, 146 publications were initially retrieved, of which 16 studies with 1198 in the BOPPPS group and 1122 in the control group were finally included. The pooled result revealed that BOPPPS was associated with a significantly improved final examination score (Standardized mean difference: 1.14, 95% CI 0.84–1.43; P < 0.001) compared to other traditional teaching. Besides, BOPPPS conferred better student satisfaction, classroom interaction, and learning initiative among medical students. The findings underscore the potential of the BOPPPS teaching strategy to enhance various aspects of medical education, but further research is warranted to explore its effectiveness in diverse cultural contexts and its impact on higher-order cognitive skills.