bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the Role of Aggrecan in Parvalbumin Interneurons: Unexpected Outcomes from a Conditional ACAN Knockout That Eliminates WFA+ Perineuronal Nets
Authors: Grødem Sverre , Thompson Elise H. , Røe Malin B. , Vatne Guro H. , Nymoen Ingeborg , Buccino Alessio , Madland Tarjei , Hafting Torkel , Fyhn Marianne , Lensjø Kristian K. 
Published: March 2024

AbstractThe transition from juvenile to adult is accompanied by the maturation of inhibitory parvalbumin-positive (PV+) neurons and reduced plasticity. This transition involves the formation of perineuronal nets (PNNs), a dense configuration of the extracellular matrix that predominantly envelops parvalbumin-positive (PV+) neurons. Aggrecan, a proteoglycan encoded by the ACAN gene, has been shown to have a key role in the PNNs as knock-out of ACAN in the adult brain reactivates juvenile plasticity, but the contribution of different cell populations is unknownHere, we establish and characterize a mouse model in which ACAN is selectively knocked out (KO) in PV+ neurons (ACANflx/PVcre). Moreover, we develop a viral tool to perform similar cell-type directed KO in adult mice. Both models are compared with the traditional method of PNN removal, namely enzymatic degradation of PNNs with Chondroitinase ABC (chABC).We show that PV+ neurons in adult ACANflx/PVcre mice do not produce PNNs that are labeled byWisteria floribunda agglutinin(WFA), the most commonly used PNN marker. Surprisingly, electrophysiological properties of PV+ interneurons in the visual cortex (V1) and ocular dominance plasticity of adult ACANflx/PVcre mice were similar to controls. In contrast, AAV-mediated ACAN knockout in adult mice increased ocular dominance plasticity. Moreover, in vivo chABC treatment of KO mice resulted in reduced firing rate of PV+ cells and increased frequency of spontaneous excitatory postsynaptic currents (sEPSC), a phenotype associated with chABC treatment of WT animals. This suggests compensatory mechanisms in the germline KO. Indeed, qPCR of bulk tissue indicates that other PNN components are expressed at higher levels in the KO animals. Finally, we perform memory-and behavioral testing to see if the lack of ACAN from PV+ neurons throughout development affected stereotypic behaviors and memory processing. ACANflx/PVcre mice have learning and memory abilities similar to controls, but use bold search strategies during navigation in the Morris water maze. The low level of anxiety-related behavior is confirmed in an open field and zero maze, where they spent nearly twice the time in open areas.
Loss of prohibitin 2 in Schwann cells dysregulates key transcription factors controlling developmental myelination
Authors: Wilson Emma R. , Nunes Gustavo Della-Flora , Shen Shichen , Moore Seth , Gawron Joseph , Maxwell Jessica , Syed Umair , Hurley Edward , Lanka Meghana , Qu Jun , Desaubry Laurent , Wrabetz Lawrence , Poitelon Yannick , Feltri M. Laura 
Published: March 2024

AbstractSchwann cells are critical for the proper development and function of the peripheral nervous system, where they form a mutually beneficial relationship with axons. Past studies have highlighted that a pair of proteins called the prohibitins play major roles in Schwann cell biology. Prohibitins are ubiquitously expressed and versatile proteins. We have previously shown that while prohibitins play a crucial role in Schwann cell mitochondria for long-term myelin maintenance and axon health, they may also be present at the Schwann cell-axon interface during development. Here, we expand on this work, showing that drug-mediated modulation of prohibitinsin vitrodisrupts myelination and confirming that Schwann cell-specific ablation of prohibitin 2 (Phb2)in vivoresults in early and severe defects in peripheral nerve development. Using a proteomic approachin vitro, we identify a pool of candidate PHB2 interactors that change their interaction with PHB2 depending on the presence of axonal signals. Furthermore, we showin vivothat loss ofPhb2in mouse Schwann cells causes ineffective proliferation and dysregulation of transcription factors EGR2 (KROX20), POU3F1 (OCT6) and POU3F2 (BRN2) that are necessary for proper Schwann cell maturation. Schwann cell-specific deletion ofJun, a transcription factor associated with negative regulation of myelination, confers partial rescue of the development defect seen in mice lacking Schwann cellPhb2. This work develops our understanding of Schwann cell biology, revealing thatPhb2may directly or indirectly modulate the timely expression of transcription factors necessary for proper peripheral nervous system development, and proposing candidates that may play a role in PHB2-mediated integration of axon signals in the Schwann cell.
Analysis and culturing of the prototypic crAssphage reveals a phage-plasmid lifestyle
Authors: Schmidtke Danica T. , Hickey Angela S. , Liachko Ivan , Sherlock Gavin , Bhatt Ami S. 
Published: March 2024

SummaryThe prototypic crAssphage (Carjivirus communis) is one of the most abundant, prevalent, and persistent gut bacteriophages, yet it remains uncultured and its lifestyle uncharacterized. For the last decade, crAssphage has escaped plaque-dependent culturing efforts, leading us to investigate alternative lifestyles that might explain its widespread success. Through genomic analyses and culturing, we find that crAssphage uses a phage-plasmid lifestyle to persist extrachromosomally. Plasmid-related genes are more highly expressed than those implicated in phage maintenance. Leveraging this finding, we use a plaque-free culturing approach to measure crAssphage replication in culture withPhocaeicola vulgatus, Phocaeicola dorei,andBacteroides stercoris, revealing a broad host range. We demonstrate that crAssphage persists with its hosts in culture without causing major cell lysis events or integrating into host chromosomes. The ability to switch between phage and plasmid lifestyles within a wide range of hosts contributes to the prolific nature of crAssphage in the human gut microbiome.
Thermal equation of state of rhodium to 191 GPa and 2700 K using double-sided flash laser heating in a diamond anvil cell
Authors: McHardy J. D. , Storm C. V. , Duff M. J. , Lonsdale C. M. , Woolman G. A. , McMahon M. I. , Giordano N. , MacLeod S. G. 
Published: March 2024

The phase behavior of rhodium (Rh) metal has been studied to 191 GPa and 2700 K using a combination of room-temperature isothermal compression and double-sided flash laser heating experiments. The isothermal compression data have been fitted with a second-order adapted polynomial of order L equation of state (EoS) with best-fitting parameters of V0=13.764(2)Å3/atom, K0=258(3)GPa, and K′=5.36(9). Two-dimensional maps of the uniaxial stress component t are presented for Rh at different pressures showing the spatial distribution of the local stress state of a relatively high-yield strength material encased in a Bi pressure medium. In addition, a simple, thermal pressure equation-of-state model, based on a single Einstein temperature, has been fitted to the high-pressure-temperature data up to 2700 K at 148 GPa and ambient-pressure thermal expansion data up to 1982 K. Also determined are the best-fitting parameters to reproduce the thermal EoS within the two-dimensional integration software. The optimized parameters are V0=13.764Å3/atom, K0=260.54GPa, K′=5.114, αT=2.99×10−5 K−1, ∂αT/∂T=1.27×10−9 K−2, ∂K0/∂T=−6.43×10−5GPa/K, and ∂K′/∂T=−9.3×10−10K−1.

Published by the American Physical Society
2024
Driving forces behind the adoption of IEQ principles in building design: evidence from Nigerian quantity surveying firms
Authors: Aliu John , Agbaje Doyin Hellen , Oke Ayodeji Emmanuel , Ebekozien Andrew 
Published: March 2024

PurposeThe main objective of this study is to evaluate the driving forces behind the adoption of indoor environmental quality (IEQ) principles in building designs from the perspectives of Nigerian quantity surveying firms.Design/methodology/approachA quantitative approach was used which involved administering a well-structured questionnaire to a sample of 114 quantity surveyors. The collected data were analyzed using various statistical methods, including frequencies, percentages, mean item scores, Kruskal–Wallis test and exploratory factor analysis.FindingsThe top five ranked drivers were climate change mitigation, conservation of natural resources, reduction of waste and pollution, use of sustainable building materials and development of new materials and building systems. Based on the factor analysis, the study identified five clusters of drivers: (1) health and well-being drivers (2) economic drivers (3) environmental drivers (4) innovation and technology drivers and (5) regulatory drivers.Practical implicationsThe findings from this study suggest that to effectively integrate IEQ principles, quantity surveying firms should consider developing comprehensive guidelines and checklists that align with the identified drivers and clustered categories. These resources can serve as practical tools for project teams, facilitating a structured and holistic approach to the incorporation of IEQ factors throughout the project lifecycle.Originality/valueThe study’s identification of the top drivers and the subsequent clustering of these drivers into five distinct categories contributes to the existing body of knowledge on IEQ. This approach provides a structured framework for comprehensively understanding the factors influencing IEQ adoption, offering a valuable tool for researchers, policymakers and industry practitioners.
An observational study of teachers' and students' behaviors in synchronous online classrooms
Authors: González Carlos , Ponce Daniel 
Published: March 2024

PurposeThis paper aims first to describe the most prevalent teachers’ and students’ behaviors in synchronous online classes in emergency remote teaching; second, to discern behavior profiles and third, to investigate what features explain the observed behaviors.Design/methodology/approachAn adapted COPUS observation protocol was employed to observe 292 online classes from 146 higher education teachers.FindingsThe most prevalent behaviors were: Presenting for teachers and Receiving for students, followed by Teachers Guiding and Students Talking to Class. Furthermore, cluster analysis showed two groups: Traditional and Interactive. The variables that better explained belonging to the Interactive lecture group were disciplinary area – social sciences and humanities –and teaching in technical institutions.Practical implicationsIn a context where higher education institutions intend to project the lessons learned into post-pandemic learning experiences, this study provides observational evidence to realize the full potential expected from online and blended teaching and learning.Originality/valueDespite the prevalence of synchronous online lectures during COVID-19, there is a paucity of observational studies on the actual behaviors that occurred in this context. Most research has been based on surveys and interviews. This study addresses this gap.
Leading the sustainable dimension of business – the responsible leadership way
Authors: Janaswamy Kiranmai , Sarkar Shulagna , Mishra Ram Kumar , Das Gupta Ananda 
Published: March 2024

Purpose
The word “sustainability” is discussed in several ways since MDGs and SDGs have gained business attention. Sustainability is not just about something’s durability; it also looks at how to advance development projects without endangering the development of others nearby, both now and in the future (Fink, 2000). It is a drive that weaves together the different facets, including the social, economic, and environmental. World Economic Forum’s has emphasised the need for responsive and responsible leadership. It is in this context, the paper focuses on studying the transformational shift in the role and responsibility of leaders in demonstrating responsible leadership and discusses the ‘Locust and Honeybee Leadership styles’.

Design/methodology/approach
Literature portrays that responsible leaders are recognized by their qualities, competency and skills. One such model is “Locust and Honeybee Leadership,” where the two leadership styles are contrasted. The paper attempts to discuss the varied contexts of these leadership styles and attempts to draw a matrix on the commonalities of both set in an Indian context. A leader should always have openness of thought and integrity.

Findings
Value systems are important for a leader. The major challenge is developing globally responsible leaders and managers who can perform effectively under these conditions of enormous challenge, as they are chronically pushed beyond their capabilities into unexpected roles and unfamiliar domains. Situations compel leaders to make decisions, yet the styles determine the way the decisions are taken. The paper is unique as it discusses the commonalities and contrasting perspectives of the “Locust and Honeybee Leadership.”

Research limitations/implications
The paper is limited to a small number of respondents and is set in Indian context.

Originality/value
To the best of the authors’ knowledge, the paper is an original piece of work and expresses thoughts of the authors on establishing a sustainable leadership model.
Enabling successful change in a high-demand working environment: a case study in a health care organization
Authors: Clarke Emma , Näswall Katharina , Wong Jennifer , Pawsey Fleur , Malinen Sanna 
Published: March 2024

PurposeThe anticipation of organizational change and the transition process often creates uncertainty for employees and can lead to stress and anxiety. It is therefore essential for all organizations, especially those that operate in high-demand working environments, to support the well-being of staff throughout the change process.Design/methodology/approachResearch on how employees respond to the organizational change of relocating to a new work space is limited. To fill this gap in the research, we present a case study examining the well-being of clinical and health care employees before and after a disruptive change: relocation in workplace facilities. In addition, factors that enabled successful change in this high-stress, high-demand working environment were investigated. Interviews were conducted with 20 participants before the relocation and 11 participants after relocation. Following an inductive approach, data were analyzed using thematic analysis to identify key themes.FindingsOur findings suggest that a supportive team, inclusive leadership and a psychologically safe environment, may buffer negative employee well-being outcomes during disruptive organizational change.Originality/valueThis research contributes to the literature on successful organizational change in health care by highlighting the resources which support well-being throughout the change process and enabling the successful transition to a new facility.
Downside risk in Dow Jones equity markets: hedging and portfolio management during COVID-19 pandemic and the Russia–Ukraine war
Authors: Said Amira , Ouerfelli Chokri 
Published: March 2024

PurposeThis paper aims to examine the dynamic conditional correlation (DCC) and hedging ratios between Dow Jones markets and oil, gold and bitcoin. Using daily data, including the COVID-19 pandemic and the Russia–Ukraine war. We employ the DCC-generalized autoregressive conditional heteroskedasticity (GARCH) and asymmetric DCC (ADCC)-GARCH models.Design/methodology/approachDCC-GARCH and ADCC-GARCH models.FindingsThe most of DCCs among market pairs are positive during COVID-19 period, implying the existence of volatility spillovers (Contagion-effects). This implies the lack of additional economic gains of diversification. So, COVID-19 represents a systematic risk that resists diversification. However, during the Russia–Ukraine war the DCCs are negative for most pairs that include Oil and Gold, implying investors may benefit from portfolio-diversification. Our hedging analysis carries significant implications for investors seeking higher returns while hedging their Dow Jones portfolios: keeping their portfolios unhedged is better than hedging them. This is because Islamic stocks have the ability to mitigate risks.Originality/valueOur paper may make a valuable contribution to the existing literature by examining the hedging of financial assets, including both conventional and Islamic assets, during periods of stability and crisis, such as the COVID-19 pandemic and the Russia–Ukraine war.
Analyzing the concept of participatory learning: strategies, trends and future directions in education
Authors: Dara Vijaya Lakshmi , Kesavan Chitra 
Published: March 2024

PurposeThe research questions probe into the heart of participatory learning’s scholarly landscape. They inquire about historical trends, shifts in research interest, and regional contributions, highlighting the global reach of participatory learning discourse. The study also reaches into the most cited papers, emphasizing their pivotal role in shaping the field’s trajectory. Design/methodology/approachThe research design of our study involves a retrospective analysis focused on the topic of participatory learning in education. A retrospective analysis is a methodological approach that entails examining existing literature, data, and publications within a specific field to identify trends, patterns, and insights over a defined period. In the context of participatory learning in education, this research design allows us to gain a comprehensive understanding of the evolution, contributions, and emerging directions within this pedagogical approach.FindingsThe culmination of this study lies in formulating 75 thought-provoking future research questions. These questions are designed to guide the future trajectory of participatory learning research, addressing existing gaps and propelling the field toward new horizons. The findings of the study contribute to a panoramic view of participatory learning’s evolution, global impact, and potential for reshaping education. It is a compass for researchers, educators, and policymakers navigating the intricate landscape of participatory learning in education. Research limitations/implicationsThemes and topics are thoroughly explored, unrevealed the multidimensional nature of participatory learning research. The investigation spans time, revealing enduring themes while unveiling emerging areas of inquiry that capture the attention of researchers and practitioners. Current trends and focus areas are scrutinized, offering insights into the ever-evolving scholarly pursuits within participatory learning.Practical implicationsThis comprehensive study investigates the evolution and impact of research on participatory learning in education from 1995 to 2023. The research objectives encompass a broad spectrum, including trend analysis, geographical distribution assessment and identification of influential papers, thematic exploration, and the formulation of future research questions. Through a meticulous examination of published literature, this study aims to shed light on the development of participatory learning as a transformative educational approach.Originality/valueThis study’s originality lies in its comprehensive analysis of participatory learning in education from 1995 to 2023, revealing temporal trends, global contributions, influential papers, thematic nuances, and emerging trends. By amalgamating these dimensions, it offers a holistic view of participatory learning’s evolution, impact, and future directions, enriching the understanding of this transformative educational approach and guiding further research and practice.
The impact of marketing appeals on consumer willingness to participate in virtual CSR co-creation
Authors: Zhang Jiemei , Tang Bingxin , Lyu Bei , Song Zhaoran 
Published: March 2024

PurposeThis study explores how businesses can effectively market functional and emotional benefits through virtual corporate social responsibility co-creation (VCSRC) initiatives. The aim is to enhance customer engagement through these initiatives.Design/methodology/approachThe initial phase of this study involved recruiting 185 Chinese university students as participants. These individuals were randomly distributed into four distinct experimental groups, each designed to investigate the influence of varied marketing appeals and common in-group identity (CIGI) on consumer engagement willingness. This phase also sought to elucidate the mediating role of CSR associations in these dynamics. Following this, the second study engaged 570 individuals, recruited through “Credamo,” for a group-based experiment. This subsequent phase was intended to validate the findings of the preliminary study and explore the varying intensities of interaction between different marketing appeals and CIGI, with a particular focus on the dichotomy of independent and interdependent self-construals.FindingsThe study delineates a detailed relationship between consumers' willingness to participate in VCSRC, marketing appeals, and common in-group identity, revealing that strong common in-group identity correlates with a preference for functional appeals, while a weaker in-group identity inclines towards emotional appeals. Notably, interdependent self-construal significantly influences consumer responses, intensifying the interaction between in-group identity and marketing appeal and thereby influencing participation willingness. Moreover, CSR associations emerge as key mediators in this interaction, underscoring their role in enhancing the effectiveness of VCSRC strategies. These insights provide a new understanding of the crucial impact of consumer identity traits on marketing strategy efficacy.Originality/valueThis research stands as a trailblazing endeavor in evaluating the effects of varied advertising appeals under the VCSR paradigm. It probes into the foundational mechanisms, leveraging insights from interaction alignment theory and persuasion theory to elucidate the processes involved.
Formation and hazard of ethyl carbamate and construction of genetically engineered Saccharomyces cerevisiae strains in Huangjiu (Chinese grain wine)
Authors: Li Mi , Jia Wei 
Published: March 2024

AbstractHuangjiu, a well‐known conventional fermented Chinese grain wine, is widely consumed in Asia for its distinct flavor. Trace amounts of ethyl carbamate (EC) may be generated during the fermentation or storage process. The International Agency for Research on Cancer elevated EC to a Class 2A carcinogen, so it is necessary to regulate EC content in Huangjiu. The risk of intake of dietary EC is mainly assessed through the margin of exposure (MOE) recommended by the European Food Safety Authority, with a smaller MOE indicating a higher risk. Interventions are necessary to reduce EC formation. As urea, one of the main precursors of EC formation in Huangjiu, is primarily produced by Saccharomyces cerevisiae through the catabolism of arginine, the construction of dominant engineered fermentation strains is a favorable trend for the future production and application of Huangjiu. This review summarized the formation and carcinogenic mechanism of EC from the perspectives of precursor substances, metabolic pathways after ingestion, and risk assessment. The methods of constructing dominant S. cerevisiae strains in Huangjiu by genetic engineering technology were reviewed, which provided an important theoretical basis for reducing EC content and strengthening practical control of Huangjiu safety, and the future research direction was prospected.