bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple pilomatricomas associated with myotonic distrophy
Authors: Calderon María J. , Manrique Pilar , Gonzalez Rosario , Garcia‐Monco JC , Zabalza I. 
Published: July 2006

AbstractCalcifying epithelioma of Malherbe, pilomatricoma, is a benign neoplasm of the skin, originating from primitive cells of the hair matrix, Myotonic dystrophy (Steinert's disease) is an uncommon hereditary disease characterized by myotonia, muscle wasting, cataracts, testicular atrophy and frontal baldness. We present the case of a woman with pilomatricomas and myotonic dystrophy and then we review the relationship between these two disorders.
Effectiveness and usability of short‐time treatment of psoriasis vulgaris with two different corticosteroids under occlusion with a hydrocolloid dressing (ContreetR)
Authors: Faber W.R. , van Joost Th. , de Hoop D. , Berretty P.J.M. , Boelen R.E. , Neumann H.A.M. 
Published: July 2006

AbstractObjectives To evaluate the efficacy of treatment of plaque‐type psoriasis with a moderate and a strong corticosteroid under continuous hydrocolloid occlusion, with weekly change of dressing, compared with treatment with the same corticosteroids alone, and the acceptance by the patients of his type of treatment.Setting Dermatological Departments of two University Hospitals and four General Hospitals.Design Open parallel study divided in two parts depending on the two different corticosteroid preparations applied. Each part was an intrasubject half‐side study with left‐right comparison.Subjects Fifty five patients with symmetrical, stable psoriasis plaques on elbows or knees.Methods Twenty nine patients were treated with clobetason butyrate cream (EmovatcR) twice daily as well as once weekly covered with a hydrocolloid dressing (ContreetR) for four weeks. Twenty six patients were treated with clobetasol propionate cream (DermovateR) twice daily as well as once weekly covered by the hydrocolloid dressing for 2 weeks. Induration, erythema and scaling were assessed using a four‐point scale. Plaque size was measured by multiplying the largest and the smallest diameter. Healing was defined as score 0 for scaling and induration and score 0 or 1 for erythema. Acceptance was evaluated by questions related to the wearing of the dressings: comfort of wearing, pain, itching, adhesiveness and sticking to clothing, and the performance of daily activities.Statistics analysis Sign test for the score values, parametric paired Student t‐test for plaque size, chi‐square test for healing.Results With occlusion a statistically significant increased reduction of severity was obtained compared to treatment without occlusion; this effect was more pronounced with the application of the strong corticosteroid. The questions related to the wearing of the dressing were answered positively by the majority of the patients.Conclusions Treatment of psoriasis plaques with, especially strong, corticosteroid creams under occlusion with a hydrocolloid dressing with a high adherence is rapid and effective. The acceptance of this type of treatment by the patients is high, and it permits normal daily activities.
Increased IgE‐levels in bullous pemphigoid correlate to soluble low affinity Fc‐II‐receptor for IgE and soluble IL‐2‐receptor
Authors: Schaller J. , Schult R. , Jeromin T. 
Published: July 2006

AbstractBackground Increased serum‐IgE levels in patients with bullous pemphigoid (BP) are one of the well known immunological deviations in this autoimmune dermatosis.Aim and methods Since the mechanism that leads to an increase of IgE in BP is still unclear, we investigated serum‐IgE levels and IgE regulating cytokines (soluble low affinity Fc II receptor for IgE, IL‐4, IFN‐g and soluble IL‐2 receptor) in sera and blister fluids of 15 BP patients before and during treatment with immunosuppressants using an ELISA‐technique.Results All 15 patients examined proved to have high IgE levels in blister fluids (> 100 KU/l) and 13 of the 15 patients had elevated serum‐IgE levels. Soluble low affinity Fc II receptor for IgE (sCD23R) was also increased in sera (7.9 ± 2.4 pg/ml) and ten fold higher in blister fluids (76.7 ± 15.3 pg/ml). Investigation of cytokines interfering with IgE production showed significantly increased levels of the soluble IL‐2 receptor in sera whereas the concentrations of IL‐4 and IFN‐g revealed no significant differences compared to controls. In blister fluids we detected all the cytokines investigated. During treatment with immunosuppressants the elevated concentrations of sCD23R and soluble IL‐2 receptor were reduced to normal values.Conclusions The results show a direct correlation between IgE levels, sCD23R and soluble IL‐2‐receptor concentrations and suggest a connection between increased IgE levels and T‐cell activation in BP.
Detection of Borrelia burgdorferi in skin biopsies from patients with morphea by polymerase chain reaction
Authors: Trevisan Giusto , Stinco Giuseppe , Nobile Carla , Bonin Serena , Stanta Giorgio 
Published: July 2006

AbstractAim We looked for the evidence of Borrelia infection in patients with morphea by serologic means and by polymerase chain reaction (PCR) analysis of skin biopsy samples.Background The possible relationship between Lyme borreliosis and morphea has been suggested by certain clinical, immunological and microbiological findings, but many authors were not be able to demonstrate Borrelia burgdorferi infection in patients with morphea and cast doubts on an etiological role for B. burgdorferi in this skin lesion.Patients and methods Ten patients with morphea, 9 females (range: 8–65 years) and one 44‐year‐old man were examined. Serological tests for Lyme borreliosis were performed by immunofluorescence assay and flagellin enzyme‐linked immunosorbent assay. Skin biopsy specimens were taken from the periphery of morphea lesions for histological examination and PCR.Results Antibodies to B. burgdorferi were detected in 3 patients and B. burgdorferi DNA was demonstrated in 5 patients.Conclusions The amplification of DNA with PCR analysis seems to open new prospects for the detection of Borrelia genome in tissues, in the present study we were able to demonstrate the presence of B. burgdorferi DNA in patients with morphea, even in seronegative patients. These data confirm that PCR is an interesting tool in skin lesion diagnosis and support the hypothesis of an etiological association between B. burgdorferi infection and some cases of morphea.
Coping and psoriasis – a framework for targeted intervention
Authors: Metz D. , Jemec G.B.E. 
Published: July 2006

AbstractBackground Dermatological patients often suffer from psycho‐social problems in relation to their disease. These problems may affect the outcome of the treatment and should therefore be dealt with in an appropriate way. A framework suitable for the analysis of psycho‐social problems of disease exists in psychological literature, and can be applied to dermatological patients in particular.Methods Examples of qualitative analysis are presented. The examples are based on focused, semi‐structured interviews transcribed into case stories. Four patients with long‐standing psoriasis vulgaris were studied.Results The coping strategies used by patients are identified using the proposed framework. The cases show that minimising is a much used strategy by patients with psoriasis. The underlying mechanisms are analysed. The analysis can serve as background for additional psycho‐social intervention on the key points of Appraisal and Sense of Coherence, which are the processes of recognising the personal significance of situations and the ability to understand the significance of a given situation in a meaningful way.Conclusion The presented model of coping is simple and allows rational and explicit identification of the required mode of psychological intervention necessary in order to offer the best possible help to patients.
Variations in the course of recurrent aphthous stomatitis in women taking hormone replacement therapy or oral contraceptives
Authors: McCartan Bernard E. , Sullivan Aine 
Published: July 2006

AbstractAim To investigate the outcome of therapeutic ovarian hormones on concurrent aphthous stomatitis.Background Studies of therapeutic hormones in recurrent aphthous stomatitis have shown conflicting results, mostly not amenable to statistical analysis.Methods 114 clients attending a well woman clinic were evaluated by means of a structured interview.Results Women with menstrual cycle associated aphthae were more likely to have complete or partial remission when receiving therapeutic ovarian hormones; women with menstrual cycle unassociated aphthae were more likely to have no change or exacerbation. These differences were statistically significant (p < 0.001).Conclusions The findings are consistent with the postulated role of ovarian hormones in the aetiology of menstrual cycle associated aphthous stomatitis.
A rare variant of incontinentia pigmenti ‐ disseminated papules and vesicles
Authors: Schaller J. , Schaller Sibylle 
Published: July 2006

AbstractThe case report of a 2 week‐old Turkish girl suffering from incontinently pigmenti (IP) with atypical disseminated papules and vesicles as a rare clinical manifestation at the onset is presented.The diagnosis result from the histological examination with eosinophilic spongiosis, the peripheral blood eosinophilia and the development of typical striated lesions is the course of the disease.
Papillon‐Lefevre syndrome in two sisters
Authors: De Giorgi V. , Martini L. , Prignano F. , Donati E. , Moretti S. 
Published: July 2006

AbstractPapillon‐Lefevre syndrome is a rare disorder of keratinization inherited as an autosomal recessive trait. It is characterized by thickening of palms and soles, perindonlopathia, tendency to pyogenic skin infections and sometimes mental impairment. The authors report Papillon‐Lefevre syndrome in two sisters in whom the familial pedigree shows the autosomal recessive inheritance of the trait. Two oilier important disorders of keratinization transmitted by an autosomal recessive gene. Richner‐Hanhart syndrome and Mal de Meleda, are excluded by clinical and metabolic criteria. Systemic therapy with etretinate and acitretin could not be performed because one of the patients has a hepatopathy and the other refuses the treatment. Application of local keratolytics is giving quite good results.
Dowling‐Degos disease localized in the vulvar area
Authors: Sacerdoti G. , Amantea A. , Donati P. , Menaguale G. , Fazio M. 
Published: July 2006

AbstractThe authors present a case of a woman with Dowling‐Degos disease in which the reticular pigmentation clearly involved the perianal and vulvar area. The extreme rarity of this localization and the importance of recognizing this disease in the mucosa is emphasized to permit identifying forms with few cutaneous characteristics and to distinguish them from other dermatoses.
Acquired reactive perforating collagenosis following diclofenac therapy in a patient with parapsoriasis
Authors: Bugatti Leonardo , Filosa Giorgio , Ciattaglia Giovanni 
Published: July 2006

AbstractA case of acquired reactive perforating collagenosis in a 70‐year old man with a long standing digitate parapsoriasis is reported. Multiple asymptomatic cutaneous hyperpigmented papules with a central adherent keratotic plug developed simultaneously with widespread purpuric eruption following a single i.m. diclofenac injection. Histologic examination of a keratotic lesion revealed an epidermal crater with centrally degenerated collagen and elastic fibre extrusion. This case is interesting for the lack of pruritus, the transepithelial elimination of both collagen and elastic fibres and the association with parapsoriasis. The etiologic role of diclofenac is discussed. The term acquired perforating dermatosis may be more suitable for this case.
Tacalcitol ointment is an efficacious and well tolerated treatment for psoriasis
Authors: Scarpa Carmelo 
Published: July 2006

AbstractAim To demonstrate the efficacy and tolerability of tacalcitol ointment on plaque psoriasis.Subject Vitamin D3 derivatives are now on world trial for plaque psoriasis treatment and we submit the results of a multicenter Italian trial (13 University and Hospital centers) with topical tacalcitol ointment, one of these new derivatives, which has less effect on calcium metabolism.Method A multicenter, randomized, double‐blind, left‐right compared 6 weeks clinical study was performed using tacalcitol ointment (4 μg/g) and betamethasone valerate ointment (0. 1%) on 76 Italian subjects with plaque‐type (vulgar) psoriasis.Results 63 subjects completed the treatment. The overall evaluation of our data gave a roughly equal improvement of plaque psoriasis with both types of treatment.Conclusions A new drug for topical treatment of plaque psoriasis vulgaris is now available: tacalcitol ointment.
In vitro cytotoxicity of antimicrobial agents to human keratinocytes
Authors: Kautzky Florian , Hartinger Anton , Köhler Lars D. , Vogt Hermann‐J. 
Published: July 2006

AbstractBackround The degree of bacterial contamination in the wound bed plays a key role in determining the level of “take” of keratinoeyte grafts in burn patients. This study was done to evaluate the best suited antimicrobial agent for topical application in relation to antimicrobial activity and cytotoxic effect on graft cells.Objective The cytotoxicity of 7 antibacterial agents (gentamicin sulphate, ciprofloxacin hydrochioride, bacitracin, polymyxin B sulphate, diethanolamin salt of fusidic acid, teicoplanin, vancomycin hydrochtoride), one anlifungal substance (amphotcricin B sodium deoxycholate) and one antiseptic agent (povidone iodine) on cultured human keratinocytes, HaCaT keratinocytes and fibroblasts was investigated.Material and methods The effects of these agents were tested using two separate assays ‐ the neutral red assay and the Bradford protein assay. In order to assess toxicity of the agent, the obtained midpoint cytotoxicity (MC50) was compared to the respective minimal inhibitory concentration (MIC) for receiving the cytotoxic potential in microbiologically effective doses.Results Antibiotics effective against Staphylococcus aureus with a small potential of toxicity are Bacitracin, Fusidic acid, Vancomycin and Tcicoplanin. Gentamicin and Ciprofloxacin are effective against both Staphylococcus aureus and Pseudomonas aeruginosa with a low potential of toxicity.Conclusion The use of antimicrobial agents with a midpoint cytotoxicity (MC50) higher than the minimal inhibitory concentration (MIC) by at least a factor of 100 might reduce the risk of toxic injury to graft cells.
Cryotherapy for lentigo maligna — is clinical acumen combined with a single punch biopsy good enough for staging?
Authors: Yell J.A. , Baigrie C. , Dawber R.P.R. , Millard P.R. , Goodacre T. 
Published: July 2006

AbstractAim We aimed to clarity the effectivenes of our staging of LM over a I‐year period.Background Cryosurgery has become accepted as a simple and effective treatment for lentigo maligna (LM) but not necessarily lentigo maligna melanoma (LMM). Pigmented epithelial cells are extremely sensitive to cold injury. If adequate freezing is delivered to the proper depth into the dermal appendages, LM should be eradicated. However, if the initial staging does not detect invasion the lesion may be inadequately treated.Methods Over 1 year prospectively. all patients presenting with LM(n= 12) wore stayed clinically by experienced dermatologists and by a single punch biopsy. This was then checked by complete excision of the lesion.Results In 9 patients the clinical and punch biopsy diagnosis was confirmed after excision. Two melanomas were missed clinically but detected on punch biopsy. In one patient the punch biopsy described a “LM with probable invasion elsewhere in the lesion”. Surgical excision yielded a melanoma, 0.8 mm thick. Clark's level 4. In a second patient, punch biopsy diagnosed superficial spreading melanoma (‐SSM) in situ, confirmed on excision.Conclusions We therefore feel that clinical diagnosis combined with a single punch biopsy will diagnose invasion when present. We emphasise that cryotherapy should not be performed without punch biopsy confirmation of the clinical diagnosis.
A multicentre evaluation of the guidelines for the use of cyclosporin A in severe psoriasis
Authors: Witkamp L. , Meinardi M.M.H.M. , Bossuyt P.M.M. , Van de Kerkhof P.C.M. , Arnold W.P. , De Hoop D. , Rampen F.H.J. , Tazelaar D.J. , Kuiters G.R.R. , Hamminga B. , Boelen R.E. , Habets W.J.M. , de Zwan N. Verburgh‐Van , Bos J.D. 
Published: July 2006

AbstractBackground Controversy still exists as lo whether a dosage scheme for the treatment of severe psoriasis with cyclosporin A (CsA) should start with low dosages (3 mg/kg/day) or rather with high dosages (5 mg/kg/day).Aims In this open prospective multi‐centre trial guidelines for the use of CsA in psoriasis beginning with low dosages were evaluated. A secondary aim of the study was to elucidate factors predicting efficacy of CsA treatment.Methods Efficacy and tolerability of CsA were evaluated monthly during 16 weeks in 86 patients (56 males, 30 females, mean age 43,0 ± 14,9 years) suffering from chronic severe plaque‐type psoriasis, not responding to topical therapy (mean PASI 18.0 ± 8.1). All patients started with 3 mg/kg/day. Patients were defined as responders with a PASI reduction > 25% at month 1, ≥ 25% at month 1, ≥ 60% at month 3 and ≥ 70% at month 4. When a patient was a failure, the dose was increased by 1 mg/kg/day lo a maximum of 5 mg/kg/day.Results A gradual mean PASI reduction of 38%. 59%, 72% to 76% was reached with a mean CsA dose of 3.0, 3.2, 3.5, and 3.6 mg/kg/day at weeks 4, 8, 12 and 16. respectively. At the end of the study period, 39 patients were still on 3, 24 patients were on 4 and 15 patients were on 5 mg/kg/day. Due to subjective side‐effects 6 patients dropped out on 3 mg/kg/day and 2 on 4 mg/kg/day. Diastolic and systolic blood pressure and creatinine levels were stable. Overall, CsA was relatively well tolerated. Absence of previous therapies, low baseline PASI and failure at week 4 were predictive for higher drop‐out and failure rate and lower PASI at the end of study.Conclusions This study shows that a significant proportion of severe psoriasis patients can be treated with 3 mg/kg/day CsA with good tolerability and excellent clinical results. It is concluded that a treatment scheme with an optimal risk‐benefit ratio should start with low dosages of CsA (3 mg/kg/day).
Progressive erythrokeratodermia with deafness
Authors: Rødland Ole , Steinkjer Bjarte 
Published: July 2006

AbstractProgressive erythrokeratodermia with deafness comprises a genodermatosis belonging to the congenital erythrokeratodermias associated with sensorineural hearing loss, teeth anomalies, neurological disturbances, psychomotor and physical development retardation. We present the history of and findings in a 26‐year‐old woman suffering from this rare condition.
Cicatricial pemphigoid and lichen sclerosus
Authors: Marren P. , Neild V. , Frith P. , Wojnarowska F. 
Published: July 2006

AbstractTwo patients with clinical and laboratory evidence of co‐existing lichen sclerosus and eicatricial pemphigoid are reported. Autoimmune bullous diseases affecting the vulva may mimic lichen sclerosus but in these two patients both diseases were present. These two diseases have not previously been reported simultaneously to date.
Investigation of notalgia paraesthetica using laser Doppler velocimetry and immunohistochemistry before and after treatment with topical capsaicin
Authors: Halpern S.M. , Ahluwalia A. , Makunura C.N. , Jordan S. 
Published: July 2006

AbstractBackground Notalgia paraesthetica is an uncommon sensory neuropathy of unknown aetiology that appears to be mediated by peptidergic nerves.Objective To investigate both functional and anatomical aspects of notalgia paraesthetica using laser Doppler velocimetry and immunohistochemistry and to assess the effect of treatment on these parameters.Methods Basal blood flow and response to local heating were recorded in 7 patients with notalgia paraesthetica from within the affected area and the contralateral side as control. Biopsies were taken from both sites for immunohistochemistry with S100 protein. PGP9.5 and CD la. Measurements were repeated after treatment with topical capsaicin.Results All patients benefited from capsaicin treatment suggesting that the symptoms of notalgia paraesthetica are mediated by peptidergic sensory nerves. Basal blood How and response to local heating were generally reduced in the affected areas prior to treatment but this did not achieve statistical significance. These differences were less obvious following treatment. The density of CD1a immunoreactive epidermal Langerhans cells and small nerve fibres in the upper dermis both appeared to be slightly increased in the majority of patients within the affected area. No significant change was noted in 3 patients who were rebiopsied after treatment.Conclusions We conclude that in notalgia paraesthetica there is a localised functional abnormality of peptidergic sensory nerves. The relevance of the subtle histological findings is unclear.
Evaluation of a solution chemiluminescent hybridization assay for identification of human papillomavirus from paraffin‐embedded tissues
Authors: Didier J.M. , Mougin C. , Bettinger D. , Vallet A. , Bernard C. , Kantelip J.P. , Lab M. 
Published: July 2006

AbstractAims To evaluate the use of the Digene Hybrid CaptureTM system, a chemiluminescent hybridization assay for the analysis of low and intermediate/high risk human papillomavirus (HPV) DNA, on paraffin‐embedded tissues and to investigate possible reasons for reaction failure.Methods Fifty cervical biopsies were tested by an in situ hybridization (ISH) method using probes for HPV 6/11. 16/18, 31/33/51 and by the Digene Hybrid CaptureTM system.Results The ISH was able to detect HPV in 23 out of the 50 biopsies. Eight samples were positive with the HPV 6/11 probe, 4 with the 16/18 probe. 6 with the 31/33/51 probe and 5 had mixed infections. With the Hybrid CaptureTM system, having taken the mixed infections into account. 40 samples gave concordant results, while total and partial discordances were observed in 6 and 4 samples respectively. The relative sensitivity of the new assay was 91.6% and specificity 84.2% for low risk HPVs; and 80% and 94.3% respectively for intermediate/high risk HPVs. Agreement rates on HPV positivity for either low risk or intermediate/high risk types were 86% and 94% respectively in comparison with ISH results.Conclusion The Hybrid CaptureTM system is simple, reliable in use and suitable for routine discrimination of HPVs in tissue sections from paraffin‐embedded blocks. It might thus be of clinical value in the diagnosis and prognosis of HPV infection.
Morphea‐like cutaneous mucinosis
Authors: Noto Giuseppe , Pravatà Gabriella , Aricò Mario 
Published: July 2006

AbstractA whitish, indurated, oval plaque, lasting for 1.5 year, was observed in the right paraumbilical skin of a middle‐aged man in otherwise good health. No erythema was present or referred. Histological examination revealed massive deposits of a globular, slightly basophilic, amorphous substance in the dermis, accompanied by a slight perivascular mononuclear cell infiltrate and scattered mast cells. Special staining with Alcian blue at pH 2.5 confirmed the intense deposition of mucin involving the entire dermis. sparing epidermis, adnexa and perivascular areas. No abnormal laboratory results indicating lupus erythematosus or thyroid dysfunction were present. Morphea was excluded on clinical and histological grounds. A plaque‐like form of cutaneous mucinosis was diagnosed. Plaque‐like mucinosis and reticular erythematous mucinosis (REM) syndrome are currently considered to be the same disease, although some authors noted a different response lo treatment. Clinical presentation of our case was unusual: no erythema preceded or accompanied the plaque growth, which had a whitish, indurated, morphea‐like appearance from the beginning but with preservation of hair follicles, quite different from archetypical REM syndrome based on clinical grounds.