bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heterogeneity of small duct‐ and large duct‐type intrahepatic cholangiocarcinoma
Authors: Kinzler Maximilian N , Schulze Falko , Jeroch Jan , Schmitt Christina , Ebner Silvana , Gretser Steffen , Bein Julia , Finkelmeier Fabian , Trojan Jörg , Zeuzem Stefan , Schnitzbauer Andreas A , Demes Melanie C , Reis Henning , Wild Peter J , Walter Dirk 
Published: February 2024

AimsThe histological subtype of intrahepatic cholangiocarcinoma (iCCA) is associated with different mutational characteristics that impact clinical management. So far, data are lacking on the presence of small duct iCCA (SD‐iCCA) and large duct iCCA (LD‐iCCA) in a single patient. The aim of the current study was to determine the presence and degree of intratumoural heterogeneity of SD‐ and LD‐iCCA features in different tumour regions.Methods and resultsAll patients treated with surgically resected iCCA at Frankfurt University Hospital between December 2005 and March 2023 were retrospectively analysed. Histomorphological features of SD‐ and LD‐iCCA were evaluated by an expert hepatobiliary pathologist. Tissue samples suspicious for subtype heterogeneity were further investigated. Immunohistochemistry for N‐cadherin, S100P, MUC5AC, MUC6, TFF1 and AGR2 and mutational profiling with the Illumina TruSight Oncology 500 (TSO500) assay were performed separately for the SD‐ and LD‐iCCA regions. Of 129 patients with surgically resected iCCA, features of either SD‐ or LD‐iCCA were present in 67.4% (n = 87) and 24.8% of the patients (n = 32), respectively; 7.8% (n = 10) had histomorphological features of both SD‐ and LD‐iCCA, seven patients (5.4%) of which had sufficient formalin‐fixed, paraffin‐embedded tissue for further analysis. Heterogeneity of both subtypes could be confirmed with immunohistochemistry. In five of seven (71.4%) patients, molecular profiling revealed intratumoural differences in genetic alterations between the SD‐ and LD‐iCCA region. In one patient, a BRAF mutation (p.V600E) was found in the SD‐iCCA but not in the LD‐iCCA region of the tumour.ConclusionsA marked portion of patients with iCCA exhibits both SD‐ and LD‐iCCA in different tumour regions. In case of the presence of histopathological heterogeneity, mutational profiling should be considered to avoid missing therapeutically relevant genetic alterations.
Genetic connectivity is maintained in two insect pollinators across a human‐altered landscape
Authors: Schleimer Anna , Wittische Julian , Luttringer Amanda , Rupprecht Christina , Andrási Bálint , Ariey Hinatea , Cruz António , Gallego Gil , Lippert Stéphanie , Purnomo Chanistya Ayu , Herrera‐Mesías Fernanda , Eickermann Michael , Kharrat‐Jarboui Imen , Castelen Lorenzo , Cantú‐Salazar Lisette , Thissen Dylan , Meimberg Harald , Weigand Alexander M. , Frantz Alain C. 
Published: February 2024

Abstract
Population genetics is a valuable tool for assessing the impact of human‐altered landscapes on genetic connectivity in various species. However, when applied to insects, challenges arise due to potentially large effective population sizes (), high dispersal capacities and the recency of anthropogenic impacts.
This study assessed the population genetic structure of two pollinators across a human‐altered landscape in Luxembourg. Samples from the ashy mining bee (Andrena cineraria, N = 201) and the greater bee fly (Bombylius major, N = 637) were genotyped at 25 microsatellite loci, including a genotyping‐by‐amplicon‐sequencing approach for A. cineraria.
Despite high statistical power of > 0.002 in B. major and > 0.0025 in A. cineraria, no deviations from genetic homogeneity were detected. For both species, there was no evidence for isolation‐by‐distance or genetic clustering. Genetic homogeneity was most likely the result of high levels of gene flow that compensate for the effects of genetic drift.
Estimates of ranged between several thousand to tens of thousands, although precision was low. Simulations highlighted that genetic lag times can substantially affect our ability to detect recent (<50 generations) population differentiation when is very large. Lag times were shorter with data from 25,000 simulated di‐allelic loci, but only when sample sizes remained high.
Insect genetic studies should consider lag times due to large and ensure sample size and markers offer adequate power to reject the null hypothesis of no landscape effect on genetic connectivity.
Does the word‐writing strategy in vocabulary learning increase learning outcomes or mislead learners into overestimation?
Authors: Kakihana Shinichiro 
Published: February 2024

AbstractStudies on the effectiveness of the word‐writing strategy in vocabulary learning have yielded mixed results. However, this strategy has been widespread among learners of English as a foreign language (EFL). This study examines whether the word‐writing strategy is effective and whether learners overestimate its effectiveness. Sixty‐eight Japanese EFL learners acquired new English words through either a writing or a nonwriting condition. Immediately following the learning phase in each condition, the Behavioral Engagement Scale, the State Engagement Scale, and the global judgments of learning (global JOLs) were administered. A multiple‐choice test for form and a productive test for meaning were administered at the end. The results showed no significant differences between the conditions in terms of learning outcomes. However, the writing condition outperformed the nonwriting condition on two engagement measures and the global JOL for form retention. These results suggest that the word‐writing strategy has no effect on learning outcomes, but increases the sense of engagement during learning and misleads students into overestimating learning outcomes. EFL teachers should educate their students about the limits of this technique and recommend more effective learning strategies.
Career formation of Japanese women entrepreneurs
Authors: Lee YounHee 
Published: February 2024

AbstractThis study conducts an in‐depth analysis of Japanese women entrepreneurs' career formation, examining how they navigate the acquisition of essential capital, such as human, cultural, social, and financial, to initiate and sustain their business ventures. It delves into the societal and cultural barriers in Japan, drawing comparisons with South Korea to shed light on the factors contributing to the relatively low engagement of Japanese women in entrepreneurship. Interviews were conducted with 69 women entrepreneurs, unveiling diverse career trajectories and underscoring the significant impact of life events on their decision to pursue entrepreneurship. This study covers the numerous challenges these women face, from societal expectations to balancing family and work, and it considers how they creatively overcome them. The findings of this study not only highlight the resilience and resourcefulness of Japanese women entrepreneurs but also indicate the implications for policy and practice to foster a more supportive environment for women's entrepreneurial endeavors in Japan and similar contexts.
Distribution analysis of TRH in Bactrocera dorsalis using a CRISPR/Cas9‐mediated reporter knock‐in strain
Authors: Teng Feiyue , Guo Fengyi , Feng Jimei , Lu Yongyue , Qi Yixiang 
Published: February 2024

AbstractAlthough the study of many genes and their protein products is limited by the availability of high‐quality antibodies, this problem could be solved by fusing a tag/reporter to an endogenous gene using a gene‐editing approach. The type II bacterial CRISPR/Cas system has been demonstrated to be an efficient gene‐targeting technology for many insects, including the oriental fruit fly Bactrocera dorsalis. However, knocking in, an important editing method of the CRISPR/Cas9 system, has lagged in its application in insects. Here, we describe a highly efficient homology‐directed genome editing system for B. dorsalis that incorporates coinjection of embryos with Cas9 protein, guide RNA and a short single‐stranded oligodeoxynucleotide donor. This one‐step procedure generates flies carrying V5 tag (42 bp) in the BdorTRH gene. In insects, as in other invertebrates and in vertebrates, the neuronal tryptophan hydroxylase (TRH) gene encodes the rate‐limiting enzyme for serotonin biosynthesis in the central nervous system. Using V5 monoclonal antibody, the distribution of TRH in B. dorsalis at different developmental stages was uncovered. Our results will facilitate the generation of insects carrying precise DNA inserts in endogenous genes and will lay foundation for the investigation of the neural mechanisms underlying the serotonin‐mediated behaviour of B. dorsalis.
Characterisation of the myosin light chain kinase (MLCK) gene of Locusta migratoria and the encoded MLCK
Authors: Wei Miao , Zhang Ning , Li Xiang‐dong 
Published: February 2024

AbstractMyosin light chain kinase (MLCK) is a dedicated kinase of myosin regulatory light chain (RLC), playing an essential role in the regulation of muscle contraction and cell motility. Much of the knowledge about MLCK comes from the study of vertebrate MLCK, and little is known about insect MLCK. Here, we identified the single MLCK gene in the locust Locusta migratoria, which spans over 1400 kb, includes 62 exons and accounts for at least five transcripts. We found that the five distinct transcripts of the locust MLCK gene are expressed in a tissue‐specific manner, including three muscle‐specific isoforms and two generic isoforms. To characterise the kinase activity of locust MLCK, we recombinantly expressed LmMLCK‐G, the smallest locust MLCK isoform, in insect Sf9 cells. We demonstrated that LmMLCK‐G is a Ca2+/calmodulin‐dependent kinase that specifically phosphorylates serine 50 of locust muscle myosin RLC (LmRLC). Additionally, we found that almost all LmRLC molecules in the flight muscle and the hindleg muscles of adult locusts are phosphorylated.
Efficacy of a buccal and lingual at‐home bleaching protocol—A randomized, split‐mouth, single‐blind controlled trial
Authors: Forville Heloisa , Bernardi Laís Giacomini , Favoreto Michael Willian , Coppla Felipe , de Souza Carneiro Taynara , Coppla Fabiana Madalozzo , Loguercio Alessandro D. , Reis Alessandra 
Published: February 2024

AbstractObjectiveTo compare the color change, the risk and intensity of tooth sensitivity (TS), and gingival irritation (GI) of at‐home bleaching applied on the buccal surface only or the buccal and lingual surfaces.Materials and MethodsSixty patients with canines A2 or darker were selected and their superior arches were randomized in two groups: at‐home bleaching on the buccal‐only or on the buccal and lingual surfaces, with 7.5% hydrogen peroxide, for 1 h daily/2 weeks. The color change was evaluated at baseline, 7, 14 days, and 1 month after bleaching using shade guides scales (ΔSGU) and a spectrophotometer (ΔEAB, ΔE00, and ΔWID). Risk and intensity of TS and GI were recorded daily using visual analogic scale (0–10). Patient satisfaction was evaluated with the orofacial esthetics. Paired t‐test, McNemar's, and Wilcoxon signed‐rank test were used for data analysis (α = 5%).ResultsNeither the color change nor the risk/intensity of TS was statistically different between groups (p > 0.05). Patient satisfaction increased after bleaching for both groups (p < 0.05).ConclusionThe addition of one contact surface does not result in an increased whitening degree compared to bleaching applied solely on the buccal surface.Clinical SignificanceUnderstanding the influence of surfaces interacting with the bleaching agent is crucial for comprehending the bleaching mechanism and avoiding unnecessary material expenses. Notably, employing the buccal‐only technique is sufficient to achieve the desired efficacy.
Structural insights into the unusual core photocomplex from a triply extremophilic purple bacterium, Halorhodospira halochloris
Authors: Qi Chen‐Hui , Wang Guang‐Lei , Wang Fang‐Fang , Wang Jie , Wang Xiang‐Ping , Zou Mei‐Juan , Ma Fei , Madigan Michael T. , Kimura Yukihiro , Wang‐Otomo Zheng‐Yu , Yu Long‐Jiang 
Published: February 2024

ABSTRACTHalorhodospira (Hlr.) halochloris is a triply extremophilic phototrophic purple sulfur bacterium, as it is thermophilic, alkaliphilic, and extremely halophilic. The light‐harvesting‐reaction center (LH1–RC) core complex of this bacterium displays an LH1‐Qy transition at 1,016 nm, which is the lowest‐energy wavelength absorption among all known phototrophs. Here we report the cryo‐EM structure of the LH1–RC at 2.42 Å resolution. The LH1 complex forms a tricyclic ring structure composed of 16 αβγ‐polypeptides and one αβ‐heterodimer around the RC. From the cryo‐EM density map, two previously unrecognized integral membrane proteins, referred to as protein G and protein Q, were identified. Both of these proteins are single transmembrane‐spanning helices located between the LH1 ring and the RC L‐subunit and are absent from the LH1–RC complexes of all other purple bacteria of which the structures have been determined so far. Besides bacteriochlorophyll b molecules (B1020) located on the periplasmic side of the Hlr. halochloris membrane, there are also two arrays of bacteriochlorophyll b molecules (B800 and B820) located on the cytoplasmic side. Only a single copy of a carotenoid (lycopene) was resolved in the Hlr. halochloris LH1–α3β3 and this was positioned within the complex. The potential quinone channel should be the space between the LH1–α3β3 that accommodates the single lycopene but does not contain a γ‐polypeptide, B800 and B820. Our results provide a structural explanation for the unusual Qy red shift and carotenoid absorption in the Hlr. halochloris spectrum and reveal new insights into photosynthetic mechanisms employed by a species that thrives under the harshest conditions of any phototrophic microorganism known.
Informed Trading Intensity
Authors: BOGOUSSLAVSKY VINCENT , FOS VYACHESLAV , MURAVYEV DMITRIY 
Published: February 2024

ABSTRACTWe train a machine learning method on a class of informed trades to develop a new measure of informed trading, informed trading intensity (ITI). ITI increases before earnings, mergers and acquisitions, and news announcements, and has implications for return reversal and asset pricing. ITI is effective because it captures nonlinearities and interactions between informed trading, volume, and volatility. This data‐driven approach can shed light on the economics of informed trading, including impatient informed trading, commonality in informed trading, and models of informed trading. Overall, learning from informed trading data can generate an effective informed trading measure.
Cellular senescence in the pathogenesis of ovarian dysfunction
Authors: Harada Miyuki 
Published: February 2024

AbstractThe follicular microenvironment is crucial for normal ovarian function, and intra‐ovarian factors, in coordination with gonadotropins, contribute to its regulation. Recent research has revealed that the accumulation of senescent cells worsens the adverse environment of various tissues and plays critical roles in chronological aging and various pathological conditions. Cellular senescence involves cell‐cycle arrest, a senescence‐associated secretory phenotype (SASP), macromolecular damage, and dysmetabolism. In this review, I summarize the latest knowledge regarding the role of cellular senescence in pathological conditions in the ovary, in the context of reproduction. Specifically, cellular senescence is known to impair follicular and oocyte health in cisplatin‐ and cyclophosphamide‐induced primary ovarian insufficiency and to contribute to the pathogenesis of polycystic ovary syndrome (PCOS). In addition, cellular senescence is induced during the decline in ovarian reserve that is associated with chronological aging, endometriosis, psychological stress, and obesity, but it remains unclear whether it plays a causative role in these conditions. Finally, I discuss the potential for use of cellular senescence as a novel therapeutic target. The modification of SASP using a senomorphic and/or the elimination of senescent cells using a senolytic represent promising therapeutic strategies. Further elucidation of the role of cellular senescence in the effects of various insults on ovarian reserve, including chronological aging, as well as in pathogenesis of ovarian pathologies, including PCOS, may facilitate a new era of reproductive medicine.
Identification of CTNNB1 in the lined seahorse, Hippocampus erectus, and its function in innate immunity and cell proliferation
Authors: Zhang Lele , Wang Fang , Lv Chunhui , Yu Lanliang , Ci Xiaojing , Zhang Ning , Jia Longwu , Xue Yuanyuan , Zhang Xu , Su Xiaolei , Wang Kai 
Published: February 2024

Abstractβ‐Catenin (CTNNB) is a core downstream mediator in the Wnt/β‐catenin signaling pathway, which plays a role in immune regulation and cell differentiation. In the present study, two CTNNB genes were identified by genomic analysis of Hippocampus erectus and named He‐CTNNB1 and He‐CTNNB2. He‐CTNNB1 was more conserved than He‐CTNNB2. The full length of He‐CTNNB1 was 2369 bp, including an open reading frame of 2275 bp that encoded 737 amino acids containing 12 armadillo repeats. Phylogenetic analysis revealed that He‐CTNNB1 clusters had homologs in other bony fishes. Tissue distribution analysis showed the highest mRNA expression of He‐CTNNB1 in the ovary, liver, and kidney of the lined seahorse under normal physiological conditions. The transcription level of He‐CTNNB1 was significantly increased in the liver and kidney in response to LPS, poly I:C, and Edwardsiella tarda challenge (p < 0.05). In vitro overexpression of He‐CTNNB1 significantly upregulated the expression of key genes of the canonical Wnt/β‐catenin signaling pathway, such as axin2, cMyc, and cyclinD (p < 0.05), which increased cell proliferation during the wound‐healing process. Taken together, our results suggest that He‐CTNNB1 is essential for cell proliferation and the innate immune response in H. erectus.
Small‐scale hatchery of common carp, Cyprinus carpio L., in the highlands of Madagascar: Technical approach to improve fry production
Authors: Rakotoambinina Samuel , Desprez Damien , Andriniaina Henri Adelphe , Randriarilala Fanomezantsoa , Rakotomavo David , Rakotoasimbola Lovasoa , Andriantsoa Mamy , Godard Léo 
Published: February 2024

AbstractIn Madagascar, fish farming is a complementary activity to agriculture, developed mainly in the highlands region. The common carp, Cyprinus carpio L., is the main species farmed. The availability of fingerlings is one of the key constraints facing the sector. The present study shows the benefit of an appropriate feed and feeding regime to both broodfish and larvae/fry and also the positive impact of protecting larvae during the first stages of their life by using happa system. The combination of these technical approaches increases significantly carp fry production.
Effects of external tags on maternal postpartum, offspring body mass and breeding frequency in gray seals Halichoerus grypus
Authors: Justrabo Charity C. , den Heyer Cornelia E. , Bowen W. Don , Lidgard Damian C. 
Published: February 2024

AbstractFew studies have examined the impacts of externally fitted data‐loggers and telemetry tags on pinnipeds. We tested for instrument effects on body mass of lactating female gray seals and their offspring and probability of pupping in the next breeding season. Known‐age adult females (n = 216) were fitted with instruments in winter, spring, and fall from 1992 to 2018 at Sable Island, Nova Scotia. Of those tagged in spring and fall, 61 of 135 returning females and 59 of their offspring were weighed within 5 days postpartum and 79 pups were weighed at weaning. Instrumented females were assigned to treatments based on tag frontal area sums, tag mass, deployment duration, and acoustic tag presence compared to control females without instruments using linear mixed‐effects models. None of the treatment effects were included in the preferred models predicting birth mass of offspring or probability of breeding in the following year. The small negative effect (−3% to −7%) on postpartum maternal mass and pup weaning mass (−4.7%) for females instrumented in fall may be an artifact as longer spring deployments showed no effect. Overall, we found that the instruments deployed had no detectable negative effects on the maternal and offspring variables measured.
The TaSnRK1‐TabHLH489 module integrates brassinosteroid and sugar signalling to regulate the grain length in bread wheat
Authors: Lyu Jinyang , Wang Dongzhi , Sun Na , Yang Fan , Li Xuepeng , Mu Junyi , Zhou Runxiang , Zheng Guolan , Yang Xin , Zhang Chenxuan , Han Chao , Xia Guang‐Min , Li Genying , Fan Min , Xiao Jun , Bai Ming‐Yi 
Published: February 2024

SummaryRegulation of grain size is a crucial strategy for improving the crop yield and is also a fundamental aspect of developmental biology. However, the underlying molecular mechanisms governing grain development in wheat remain largely unknown. In this study, we identified a wheat atypical basic helix–loop–helix (bHLH) transcription factor, TabHLH489, which is tightly associated with grain length through genome‐wide association study and map‐based cloning. Knockout of TabHLH489 and its homologous genes resulted in increased grain length and weight, whereas the overexpression led to decreased grain length and weight. TaSnRK1α1, the α‐catalytic subunit of plant energy sensor SnRK1, interacted with and phosphorylated TabHLH489 to induce its degradation, thereby promoting wheat grain development. Sugar treatment induced TaSnRK1α1 protein accumulation while reducing TabHLH489 protein levels. Moreover, brassinosteroid (BR) promotes grain development by decreasing TabHLH489 expression through the transcription factor BRASSINAZOLE RESISTANT1 (BZR1). Importantly, natural variations in the promoter region of TabHLH489 affect the TaBZR1 binding ability, thereby influencing TabHLH489 expression. Taken together, our findings reveal that the TaSnRK1α1‐TabHLH489 regulatory module integrates BR and sugar signalling to regulate grain length, presenting potential targets for enhancing grain size in wheat.
Ecological interactions between farmed Atlantic salmon and wild Atlantic cod populations in Norway: A review of risk sources and knowledge gaps
Authors: Bøhn Thomas , Strøm John F. , Sanchez‐Jerez Pablo , Keeley Nigel B. , Johansen Torild , Gjelland Karl Ø. , Sandlund Nina , Sæther Bjørn‐S. , Sætra Ingeborg , Olsen Esben M. , Skjæraasen Jon E. , Meier Sonnich , van der Meeren Terje , Bjørn Pål A. 
Published: February 2024

AbstractAquaculture provides an important and expanding source of protein rich and healthy food to the world. However, to minimize environmental harm from aquaculture, interactions with wild fish communities need to be thoroughly assessed. Here, we characterize the existing knowledge pertaining to such interactions, exemplified with Atlantic salmon (Salmo salar) farming in open net pens along the Norwegian coast and potential consequences for wild Atlantic cod (Gadus morhua) populations. Importantly, the wild cod fishery also provides a protein rich, high quality food source with high economic value. We identify seven risk sources that may affect behaviour, physiology, and survival in wild cod. Of particular importance is the large amount of waste feed that causes wild fish to aggregate around farms, thereby altering a multitude of ecological interactions including predation and disease transmission. Moreover, altered food quality in pellets may alter physiological processes and cause mortality to vulnerable life‐stages in wild cod. More research is needed on mechanisms and thresholds for harm. As the most important cod fisheries are found in northern Norway, where climate change also is rapid, we expect stronger and potentially more harmful interactions between fish farming and wild cod fisheries as aquaculture continues to expand. We hope that our analysis will inspire further research, on farmed salmon and wild cod interactions, but also on aquaculture and wild fish interactions in general. Such research is fundamental for the development of management systems that can reduce the impact of aquaculture on fisheries and the environment.