bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imidazole propionate ameliorates atopic dermatitis-like skin lesions by inhibiting mitochondrial ROS and mTORC2
Authors: Kim Ha Eun , Lee Jong Yeong , Yoo Dong-Hoon , Park Hyo-Hyun , Choi Eun-Ju , Nam Kyung-Hwa , Park Jin , Choi Jin Kyeong 
Published: March 2024

BackgroundImidazole propionate (IMP) is a histidine metabolite produced by some gut microorganisms in the human colon. Increased levels of IMP are associated with intestinal inflammation and the development and progression of cardiovascular disease and diabetes. However, the anti-inflammatory activity of IMP has not been investigated. This study aimed to elucidate the role of IMP in treating atopic dermatitis (AD). MethodsTo understand how IMP mediates immunosuppression in AD, IMP was intraperitoneally injected into a Dermatophagoides farinae extract (DFE)/1-chloro-2,4 dinitrochlorobenzene (DNCB)-induced AD-like skin lesions mouse model. We also characterized the anti-inflammatory mechanism of IMP by inducing an AD response in keratinocytes through TNF-α/IFN-γ or IL-4 stimulation. ResultsContrary to the prevailing view that IMP is an unhealthy microbial metabolite, we found that IMP-treated AD-like skin lesions mice showed significant improvement in their clinical symptoms, including ear thickness, epidermal and dermal thickness, and IgE levels. Furthermore, IMP antagonized the expansion of myeloid (neutrophils, macrophages, eosinophils, and mast cells) and Th cells (Th1, Th2, and Th17) in mouse skin and prevented mitochondrial reactive oxygen species production by inhibiting mitochondrial energy production. Interestingly, we found that IMP inhibited AD by reducing glucose uptake in cells to suppress proinflammatory cytokines and chemokines in an AD-like in vitro model, sequentially downregulating the PI3K and mTORC2 signaling pathways centered on Akt, and upregulating DDIT4 and AMPK. DiscussionOur results suggest that IMP exerts anti-inflammatory effects through the metabolic reprogramming of skin inflammation, making it a promising therapeutic candidate for AD and related skin diseases.
Disease-associated immune cell endotypes in anti-MDA5-positive dermatomyositis using unbiased hierarchical clustering
Authors: Guo Ruru , Yang Yang , Gu Liyang , Li Xinyu , Ma Yiyangzi , Liu Xuesong , Lu Liangjing 
Published: March 2024

ObjectiveClinical and prognostic features of Anti-MDA5-Positive Dermatomyositis (MDA5+ DM) are diverse. This study aimed to examine the peripheral immune cell profiles of patients with MDA5+ DM, identify disease endotypes related to the heterogeneous manifestations and prognosis, and guide individualized therapy regimen.MethodsThis inpatient cohort included 123 patients with MDA5+ DM. Unsupervised hierarchical clustering analysis was used to derive disease endotypes from the circulating immune cell profiles on admission. Clinical symptoms, laboratory test results, inpatient treatments, and disease outcomes were then analyzed among the identified endotypes.ResultsThree disease endotypes in MDA5+ DM were identified from peripheral immune cell profiles. Endotype1 had the highest percentages of CD4+ T cells and monocytes, and the lowest percentage of neutrophils; Endotype2 had the highest percentage of B cells; Endotype3 had the highest percentage of CD8+ T cells and NK cells. Clinical and prognostic heterogeneity of the endotypes were revealed. Endotype1 had the lowest 3-month mortality with the high incidence of periungual capillary changes. Endotype2 and Endotype3 had higher prevalence of rapidly progressive interstitial lung disease (RPILD) and mortality at 3 months than Endotype1. Meanwhile, Endotype3 had higher pneumocystis jiroveci and CMV viremia cases with significantly elevated of activated CD8+ T cells and multiple cytokines than Endotype1.ConclusionClustering analysis of peripheral immune cell profiles identified three different endotypes in MDA5+ dermatomyositis. Endotpye2 and 3 showed higher RPILD, 3-month mortality, pneumocystis jiroveci and CMV viremia.
Yarrowia lipolytica as a promising protein source for Pacific white shrimp (Litopenaeus vannamei) diet: impact on growth performance, metabolism, antioxidant capacity, and apparent digestibility
Authors: Zhang Lei , Zhang Pian , Tan Peng , Xu Dongdong , Wang Ligai , Ding Zhili , Shao Qingjun 
Published: March 2024

Exploring alternatives to fishmeal (FM) in aquaculture nutrition remains a hot topic in this area. Yarrowia lipolytica (YL), a promising single-cell protein source, has yet to be evaluated for its use on the Pacific white shrimp (Litopenaeus vannamei) diet. This investigation assessed the effects of YL on growth performance, metabolism, antioxidant capacity, and nutrient digestibility in shrimp. Seven diets with equal protein and lipid contents were prepared, incorporating gradient YL (dry matter basis): 0% (control), 1.50%, 3.00%, 4.50%, 6.00%, 9.00%, and 12.00%. During a 56-day feeding trial involving shrimp, with an initial average weight of 0.32 ± 0.02 g, each kind of diet was randomly assigned to six tanks. It was observed that the growth performance improved initially but then exhibited a declining trend as the dietary inclusion of YL increased. Regression analysis indicated 2.68% as the optimal YL percentage for a specific growth rate (SGR), accounting for 10.54% of the dietary FM content. A higher YL (over 6.00%), which is equivalent to replacing over 23.6% of the FM, led to increased feed intake and conversion ratios, possibly correlating with decreased YL digestibility. However, YL inclusion across the studied range had no detrimental impact on intestinal or hepatopancreatic morphology. Notably, higher YL levels significantly enhanced hepatopancreatic trypsin activity and reduced serum triglyceride and cholesterol levels. The inclusion of YL notably augmented antioxidant defense mechanisms in shrimp, as evidenced by increased total antioxidant capacity and catalase activities, as well as reduced malondialdehyde levels in the hepatopancreas, possibly due to the presence of β-glucan and mannan oligosaccharides in YL. These findings support YL being viable as a protein source in shrimp feeds.
Effects of online tDCS and hf-tRNS on reading performance in children and adolescents with developmental dyslexia: a study protocol for a cross sectional, within-subject, randomized, double-blind, and sham-controlled trial
Authors: Battisti Andrea , Lazzaro Giulia , Varuzza Cristiana , Vicari Stefano , Menghini Deny 
Published: March 2024

BackgroundDevelopmental Dyslexia (DD) is a brain-based developmental disorder causing severe reading difficulties. The extensive data on the neurobiology of DD have increased interest in brain-directed approaches, such as transcranial direct current stimulation (tDCS), which have been proposed for DD. While positive outcomes have been observed, results remain heterogeneous. Various methodological approaches have been employed to address this issue. However, no studies have compared the effects of different transcranial electrical stimulation techniques (e.g., tDCS and transcranial random noise stimulation, tRNS), on reading in children and adolescents with DD.MethodsThe present within-subject, double-blind, and sham-controlled trial aims to investigate the effects of tDCS and hf-tRNS on reading in children and adolescents with DD. Participants will undergo three conditions with a one-week interval session: (A) single active tDCS session; (B) single active hf-tRNS session; and (C) single sham session (tDCS/hf-tRNS). Left anodal/right cathodal tDCS and bilateral tRNS will be applied over the temporo-parietal regions for 20 min each. Reading measures will be collected before and during each session. Safety and blinding parameters will be recordered.DiscussionWe hypothesize that tRNS will demonstrate comparable effectiveness to tDCS in improving reading compared to sham conditions. Additionally, we anticipate that hf-tRNS will exhibit a similar safety profile to tDCS. This study will contribute novel insights into the effectiveness of hf-tRNS, expediting the validation of brain-based treatments for DD.
A real-world disproportionality analysis of Everolimus: data mining of the public version of FDA adverse event reporting system
Authors: Zhao Bin , Fu Yumei , Cui Shichao , Chen Xiangning , Liu Shu , Luo Lan 
Published: March 2024

Background: Everolimus is an inhibitor of the mammalian target of rapamycin and is used to treat various tumors. The presented study aimed to evaluate the Everolimus-associated adverse events (AEs) through data mining of the US Food and Drug Administration Adverse Event Reporting System (FAERS).Methods: The AE records were selected by searching the FDA Adverse Event Reporting System database from the first quarter of 2009 to the first quarter of 2022. Potential adverse event signals were mined using the disproportionality analysis, including reporting odds ratio the proportional reporting ratio the Bayesian confidence propagation neural network and the empirical Bayes geometric mean and MedDRA was used to systematically classify the results.Results: A total of 24,575 AE reports of Everolimus were obtained using data from the FAERS database, and Everolimus-induced AEs occurrence targeted 24 system organ classes after conforming to the four algorithms simultaneously. The common significant SOCs were identified, included benign, malignant and unspecified neoplasms, reproductive system and breast disorders, etc. The significant AEs were then mapped to preferred terms such as stomatitis, pneumonitis and impaired insulin secretion, which have emerged in the study usually reported in patients with Everolimus. Of note, unexpected significant AEs, including biliary ischaemia, angiofibroma, and tuberous sclerosis complex were uncovered in the label.Conclusion: This study provided novel insights into the monitoring, surveillance, and management of adverse drug reaction associated with Everolimus. The outcome of serious adverse events and the corresponding detection signals, as well as the unexpected significant adverse events signals are worthy of attention in order to improving clinical medication safety during treatment of Everolimus.
The Virtual Reality Influence on the Eye Movement in Patients with Cerebral Infarction and Intracerebral Hemorrhage
Authors: Марьенко И.П. , Лихачев С.А. , Крамаренко А.Н. 
Published: March 2024

Введение. Медицинская реабилитация пациентов с инфарктом мозга (ИМ) и внутримозговым кровоизлиянием (ВМК) с использованием среды виртуальной реальности (ВР) является перспективным направлением в восстановлении глазодвигательных нарушений. Общность патофизиологических механизмов нарушения глазодвигательных реакций и развития других двигательных дефицитов позволяет применять анализ глазодвигательной функции как неинвазивный метод оценки состояния пациентов в динамике. Цель. Изучить влияние мультиафферентного воздействия среды ВР на показатели глазодвигательных реакций у пациентов с ИМ и ВМК. Материалы и методы. Оптокинетический нистагм (ОКН) регистрировали у 15 пациентов методом электронистагмографии до и после тренировок в среде ВР. Результаты. Установлено уменьшение величины и асимметрии амплитуды медленной фазы (Ам), амплитуды быстрой фазы (Аб), скорости медленной фазы (СМФ) и скорости быстрой фазы (СБФ) горизонтального ОКН после тренировок в среде виртуальной реальности. Заключение. Полученные результаты могут указывать на тенденцию к нормализации ритма ОКН.
Introduction. Medical rehabilitation of patients with cerebral infarction (MI) and intracerebral hemorrhage (ICH) using a virtual reality (VR) environment is a promising direction in the restoration of oculomotor disorders. Pathophysiological mechanism of oculomotor phenomena and other motor disorders pathophysiological mechanism similarity make it possible to use the analysis of oculomotor functions as a non-invasive method for assessing patient dynamics. Purpose. We aimed to study the influence of multifferent influence of the VR environment on indicators of oculomotor reactions in patients with MI and ICH. Materials and methods. Optokinetic nystagmus (OKN) was recorded in 15 patients using electronystagmography before and after training in a VR environment. Results. A decrease in the magnitude and asymmetry of horizontal OKN eye movement features after training in a virtual reality environment was established. Conclusion. It may show a tendency towards normalization of the OKN rhythm.
Possibilities of Using Transcranial Magnetic Stimulation for Migraine
Authors: Глеб О.В. , Чернуха Т.Н. , Лихачев С.А. 
Published: March 2024

Данные некоторых исследований показывают, что транскраниальная магнитная стимуляция (ТМС) позволяет снижать количество дней мигрени в месяц, уменьшать интенсивность и продолжительность приступов мигрени в течение нескольких дней после применения ТМС, быстро купировать приступ мигрени и улучшать уровень качества жизни. Кроме того, ТМС безопасна и хорошо переносится, совместима с приемом фармакологической профилактической терапии при хронической мигрени. Однако отсутствие однородности результатов и данных о долгосрочной реальной эффективности приводит к тому, что результаты не предоставляют убедительных доказательств эффективности ТМС в лечении мигрени. В целях оптимального применения ТМС в клинической практике для лечения мигрени необходимы дальнейшие исследования, направленные на уточнение механизмов влияния данной процедуры на головной мозг. Важной проблемой, препятствующей широкому клиническому применению ТМС при мигрени, является неполное знание механизмов действия ТМС при мигрени, касающееся как профилактического лечения, так и лечения острой фазы. Следовательно, необходимы проведение дальнейших рандомизированных плацебо-контролируемых исследований для выяснения патофизиологии мигрени, анализ безопасности длительного и частого применения ТМС. Также требуется изучение потенциального взаимодействия между ТМС и новыми доступными препаратами для лечения и предотвращения приступов мигрени. Актуальна разработка оптимальных параметров ритмической ТМС, количества сеансов, показаний для направления на данный вид лечения.
Some studies show that transcranial magnetic stimulation (TMS) can reduce the number of migraine days per month, reduce the intensity and duration of migraine attacks within a few days after using TMS, quickly relieve a migraine attack, and improve quality of life. In addition, TMS is safe and well tolerated and is compatible with pharmacological preventive therapy for chronic migraine. However, the lack of uniformity of results and long-term real-world effectiveness data means that the results do not provide convincing evidence of the effectiveness of TMS in the treatment of migraine. Further research is needed to clarify the mechanisms of the effect of this procedure on the brain in order to optimally use TMS in clinical practice for the treatment of migraine. An important problem preventing the widespread clinical use of TMS for migraine is incomplete knowledge of the mechanisms of action of TMS for migraine, regarding both preventive treatment and treatment of the acute phase. Therefore, further randomized placebo-controlled studies are needed to elucidate the pathophysiology of migraine, as well as to analyze the safety of long-term and frequent use of TMS. Potential interactions between TMS and new available drugs for the treatment and prevention of migraine attacks are also warranted. It is relevant to develop optimal parameters for rhythmic TMS, the number of sessions, and indications for referral to this type of treatment.
ANALISIS NILAI TAMBAH PADA USAHA AGROINDUSTRI VIRGIN COCONUT OIL (VCO)
Authors: Pratiwi Saskiah , Pellokila Marthen R. , Kapa Maximillian M.J. 
Published: March 2024

This study aims to (1) calculate the profit from processing coconut into VCO (2) calculate the efficiency (3) calculate the added value of VCO agro-industrial businesses in Kemasama Micro Enterprises. Data collection methods are carried out using profit and loss analysis, R/C ratio analysis and the Hayami method. The results of the research can be explained as follows : (1) the profit received from processing cococnut into VCO in Kemasama Micro Enterprises during 2021 is Rp.42.077.875,-, in 2022 in the amount of Rp.59.703.875,- an increase of Rp.17.626.000,- or 14%. (2) The efficiancy of coconut processing business to become VCO in Kemasama Micro Enterprises with an R/C ratio value of 2,26 in 2021 and an increase in 2022 of 2,62 this means that the business of processing coconut into VCO in Kemasama Micro Enterprises shows efficiency, where the value of the R/C ratio is more than 1 (R/C ratio > 1). (3) The added value obtained from processing cococnut into VCO in the Kemasama Micro Business is Rp.45.000 per one liter of raw material or around 75,84% of the product value per unit of raw material and profit drived from processing coconut into VCO in one production is Rp. 28.312,5,- per liter or 62,22%.
POLA PENGELUARAN MAKANAN DAN NON MAKANAN PADA KELUARGA PRA-SEJAHTERA DAN SEJAHTERA 1 DI KECAMATAN FATULEU KABUPATEN KUPANG
Authors: Budi Maria , Nendissa Doppy Roy , Hendrik Ernantje 
Published: March 2024

This study aims to determine the Expenditures and differences in the Pattern of Food and Non-Food Expenditures in Pre-Prosperous and Prosperous 1 Families in Fatuleu District, Kupang Regency. To find out food and non-food consumption expenditures, descriptive methods were used, while to find out differences in food and non-food expenditure patterns in Pre-Prosperous and Prosperous 1 Families, data analysis techniques were carried out using Chi Square Two Independent Samples. The results of the study show that (1) the average spending on food and non-food consumption in pre-prosperous families: food expenditure is Rp. 759,000 and non-food expenditure amounted to Rp. 587,000. While the average for food and non-food expenditure in the Prosperous Family 1: food expenditure amounts to Rp. 1,178,920 and non-food expenditure amounted to Rp. 898,480. Thus the Pre-Prosperous and Prosperous 1 families prioritize spending on food compared to non-food. (2) There is a difference in the pattern of food expenditure for Pre-Prosperous and Prosperous 1 families with a food value of 1,000 and a non-food value of 0.728. Thus there are significant differences in the pattern of food and non-food expenditure in Pre-Prosperous and Prosperous 1 families. The existence of significant differences in the pattern of food and non-food expenditure in Pre-Prosperous and Prosperous 1 families shows that people prioritize spending on food consumption compared to non-food consumption
Faktor Penentu Produksi Tanaman Buah Naga Merah (Hylocereus polyrhizus)
Authors: Ledoh Neta Ayu Puspita , Pellokila Marthen Robinson , Lango Alfetri N. P. 
Published: March 2024

This research was conducted in Kolobolon Village, Lobalain District, Rote Ndao District. The purpose of this study was to find out the general description of red meat dragon fruit (Hylocereus polyrhizus) cultivation techniques and to find out the factors that determine the production of red meat dragon fruit (Hylocereus polyrhizus). The variables used in this study were land area, organic fertilizers, pesticides, labor and number of plants. Using primary and secondary data. Data collection techniques with interviews and documentation methods. Data processing was carried out using the SPSS version 26 program using the Cobb-Douglas production function.The results showed that all stages of agricultural activity started from seeding, tillage, support poles, planting, fertilizing, pruning, watering, pest control, harvesting, and post-harvesting. Based on the analysis using the Cobb-Douglas production function, the following results are obtained: : Y = 7.683 X1–0,060 X20,304 X3– 0,011X40,355X50,208.
The results of the simultaneous F test showed that the independent variable had an effect on the yield of red flesh dragon fruit (Hylocereus polyrhizus) of 24.261. Partial test (t) shows that organic fertilizer, labor and the number of plants significantly affect the yield of dragon fruit with red meat (Hylocereus polyrhizus). Meanwhile, area and pesticide variables had no significant effect on the yield of red meat dragon fruit (Hylocereus polyrhizus). The Adjusted R2 value is 0.847. showed that 84.70% of red meat dragon fruit (Hylocereus polyrhizus) production could be explained by area variables, organic fertilizers, pesticides, labor and number of plants. While the remaining 15.30%, this ratio is explained by other factors that are not included in the regression model.
KARAKTERISTIK POHON INANG DAN KEANEKARAGAMAN ANGGREK (Orchidaceae) EPIFIT DI HUTAN LINDUNG LUNUNIANAM, DESA FATU’ULAN, KECAMATAN KI’E, KABUPATEN TIMOR TENGAH SELATAN, PROVINSI NUSA TENGGARA TIMUR
Authors: Abe Karmila , Pellondo’u Mamie , Mau Astin E 
Published: March 2024

This study was conducted to determine the characteristics of orchid host trees, the diversity of epiphytic orchids (Orchidaceae), and the preservation of host trees and epiphytic orchids in the Lununianam protected forest area. This research was conducted in Lununianam Protection Forest, Fatu'ulan Village, Ki'e District, South Central Timor Regency, East Nusa Tenggara Province. Which is carried out for 1 month, from November to December 2022. This study used purposive sampling methods, surveys, and cruising paths with data analysis using qualitative descriptive and quantitative descriptive analysis. Host tree data was processed using vegetation analysis formulas and orchid data was processed using the Shannon wiener (H') diversity index formula.
The results showed that there were 5 species of host trees found in the study site, namely forest guava (Syzygium pycnanthum), Matani (Pterocarpus indicus), Tahi (Celtis wightii planch), Kamala (Mallotus philippensis), Jenitri (Elaeocarpus ganitrid). Host trees found in Lununianam Protection forest have the characteristics of large hard and rough bark, have a height range of 12-30 m, and bark overgrown with moss, The results of vegetation analysis of host tree species with the highest Important Value Index namely Syzygium Pycnanthum and Elaeocarpus ganitrus have the lowest Important Value Index. Orchids found in the sampling plot in the Lununianam Protection Forest contained 8 species of epiphytic orchids, namely Dendrobium Sp, Vanda insignis, Eria multiflora, Flickingeria grandiflora, Trichoglottis bipenicillata, Pholidota carnea, Appendicula reflexa Blume, and Ceologyne speciosa. The calculation of Shannon Wiener's diversity index of 1.57 (H') is classified as medium (1<H'<3). The most common type of orchid found is the type Eria multifora with a total of 168 individuals and the type that is less commonly found is Appendicula reflexa Blume with 4 individuals.
KAJIAN KEARIFAN LOKAL MASYARKAT DALAM PENGELOLAAN HUTAN LINDUNG MUTIS TIMAU (STUDI KASUS RITUAL ADAT (HERING) DI DESA BITOBE, KECAMATAN AMFOANG TENGAH, KABUPATEN KUPANG, PROVINSI NUSA TENGGARA TIMUR)
Authors: Nuhang Clemens Januarius , Riwu Kaho Ludji Michael , Pramatana Fadlan 
Published: March 2024

Tujuan dari penelitian ini adalah untuk mengetahui bentuk dan pengaruh kearifan lokal di Desa Bitobe Kecamatan Amfoang Tengah, Kabupaten Kupang, Provinsi Nusa Tenggara Timur.
Penelitian ini dilaksanakan di Hutan Lindung Gunung Mutis Timau Desa Bitobe Kecamatan Amfoang Tengah, Kabupaten Kupang, Provinsi Nusa Tenggara Timur. Penelitian ini menggunakanwawancara bentuk tidak terstruktur Teknik pengumpulan data yang digunakan dalam penelitian ini adalah data primer dan data sekunder. Dari data yang dikumpulkan dilakukan Analisis data secara deskriptif dan kuantitatif.
Hasil penelitian masyarakat adat Desa Bitobe telah lama mendiami wilayah disekitar kawasan Hutan Gunung Timau, dari pengalaman masyarakat tutupan lahan di Hutan Timau mengalami perubahan yangdiakibatkan dari bencana alam. Kearifan lokal masyarakat Desa Timau berpengaruh terhadap kelestarian hutan, kearifan lokal masyarakat sangat melekat erat dalam praktek pola pertanian dan pemanenan hasil hutan madu. Dengan adanya kearifan lokal yang dipegang oleh masyarkat, kelestraian hutan dapat terjaga.