bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adult T‐cell leukemia (ATL) virus‐specific antibodies in atl patients and healthy virus carriers
Authors: Yamamoto N. , Schneider J. , Koyanagi Y. , Hinuma Y. , Hunsmann G. 
Published: February 2007

AbstractThe adult T‐cell leukemia (ATL)‐associated antigen complex (ATLA) is recognized by serum antibodies of carriers of ATL virus (ATLV). ATLA consists mainly of ATLV polypeptides and their precursors. The sera from 22 ATL patients, 21 healthy carriers and 9 healthy individuals were examined quantitatively by immunofluorescence assay (IF) for ATLA and by a newly developed radioimmunoprecipitation test with purified 125I‐gp68, the putative env gene product of ATLV. More qualitative results were obtained by analysis on polyacrylamide gel (PAGE) of immunoprecipitates from lysates of 35‐S‐cysteine‐la‐belled cells producing ATLV, pelleted ATLV and cell‐free culture supernatant. The two quantitative assays gave negative results with sera from all normal subjects and a few patients, but detected ATLA antibodies in all the healthy ATLV carriers. An important finding was that sera of patients that gave negative results in one assay gave positive, results in the other, and vice versa. In contrast, all sera from ATL patients and healthy carriers, but not normal donors, precipitated ATLV‐specific glycopolypeptides, gp68 and gp46 from 35S‐labelled materials. But core polypeptides p28, p24, p19 and p15 were precipitated only by sera with IF titers of over 80. Thus, anti‐ATLA antibodies in seropositive sera are predominantly directed against glycopolypeptides of ATLV, and the antibody reactivity to ATLA antigens does not differentiate between ATL patients at various stages of the disease and healthy ATLV carriers.
Monoclonal antibody (1116 NS 19‐9) defined monosialoganglioside (GICA) in colorectal carcinoma in relation to stage, histopathology and DNA flow cytometry
Authors: Arends J. W. , Wiggers T. , Schutte B. , Thijs C. T. , Verstijnen C. , Hilgers J. , Blijham G. H. , Bosman F. T. 
Published: February 2007

AbstractImmunoreactivity of 1116 NS 19‐9 monoclonal antibody defined monosialoganglioside (gastrointestinal cancer‐associated antigen, GICA) has been studied in a series of colorectal carcinoma patients of a multicentre prospective controlled trial in order to assess its correlation with parameters such as localization, stage histopathological characteristics and DNA flow cytometry. GICA could be detected in 60% of the carcinomas, but no correlation was observed between its status of immunoreactivity and any of the parameters studied. It is concluded that, though study of the expression of the monosialganglioside may be worth while in relation to fundamental aspects of behaviour of colorectal carcinomas, the significance of its immunohistochemical detection in a diagnostic or prognostic sense is limited.
Epstein‐Barr virus specific T‐cell response in nasopharyngeal carcinoma patients
Authors: Moss D. J. , Chan S. H. , Burrows S. R. , Chew T. S. , Kane R. G. , Staples J. A. , Kunaratnam N. 
Published: February 2007

AbstractThere is a substantial body of evidence suggesting an association between Epstein‐Barr virus (EBV) and undifferentiated nasopharyngeal carcinoma (NPC). The present study has compared a group of NPC patients (newly diagnosed and long‐term survivors) and controls for EBV‐specific T‐cell immunity using the regression of transformation assay. Newly diagnosed patients (17 tested) when compared with either long‐term survivors (20 tested) or controls (30 tested) showed a significant impairment in virus‐specific T‐cell immunity (p = 0.036, p = 0.043 respectively). Furthermore, donors with IgA antibody to EBV showed a significant depression in virus‐specific T‐cell immunity compared with donors without IgA antibody (19 IgA‐positive, 48 IgA‐negative; p = 0.0025). These results may be important in explaining the postulated role of EBV in the aetiology of NPC.
Purification of a protein (60K/58K) associated with the Epstein‐Barr virus‐induced early antigen complex in Raji cells
Authors: Dölken Gottfried , Lange Winand , Weitzmann Ulrike , Hirsch Friedrich W. , Löhr Georg W. 
Published: February 2007

AbstractA double antibody sandwich ELISA has been established for the detection and quantitation of EBV‐associated early antigens (EA) in IUdR‐induced Raji cells. The EA complex extracted from Raji cells could be separated by ion exchange chromatography and isoelectric focusing into several components. One EA‐associated subspecificity has been purified by DEAE‐, CM‐, and Blue‐Sepharose chromatography followed by isoelectric focusing. The isolated protein has an apparent molecular weight of 240,000 ± 20,000 daltons under non‐dissociating conditions on Sephacryl S‐300, an isoelectric point of 4.5, and seems to be composed of two polypeptides of 60,000 and 58,000 daltons as shown by SDS‐gel electrophoresis and two‐dimensional gel electrophoresis. Preliminary data indicate that the 58,000 polypeptide is generated by limited proteolysis of the 60,000 polypeptide. The EA activity of the isolated protein has been confirmed by the double antibody sandwich ELISA and its reactivity with anti‐EA‐positive sera in an ELISA for the detection of anti‐EA antibodies.
A study of gastrointestinal cancer‐associated antigen (GICA) in human fetal organs
Authors: Raux Hélène , Labbe François , Fondaneche Marie‐Claude , Koprowski Hilary , Burtin Pierre 
Published: February 2007

AbstractThe gastrointestinal cancer‐associated antigen (GICA) characterized by 1116 NS 19‐9 monoclonal antibody was studied in human fetal organs by immunohistology and immunofixation on nitrocellulose sheets. It was found in all the gastrointestinal tracts of fetuses and newborns. Other fetal organs, except biliary and pancreatic ducts, were negative. Immunohistological data and enzymatic studies led us to conclude that GICA is predominantly a mucin‐type glycoprotein in fetal organs.
Detection of serum antibodies to adult T‐cell leukemia virus in non‐human primates and in people from Africa
Authors: Hunsmann Gerhard , Schneider Josef , Schmitt Jakob , Yamamoto Naoki 
Published: February 2007

AbstractThe distribution of serum antibodies to adult T‐cell leukemia virus (ATLV) was examined as a marker for virus infection among non‐human primates as well as people from Africa and Germany. The virus is present in Africa in certain primate species including man. Altogether, 468 sera from 27 monkey species were examined. Only African green monkeys, less frequently also chimpanzees and crab‐eating monkeys, were found to be infected. About 1–2% of people from Kenya have antibodies, while ATLV‐antibodies may be present in well below 0.1% of the German population.
Natural infection in non‐human primates with adult T‐cell leukemia virus or a closely related agent
Authors: Miyoshi I. , Fujishita M. , Taguchi H. , Matsubayashi K. , Miwa N. , Tanioka Y. 
Published: February 2007

AbstractA total of 703 sera from 10 species of monkeys were examined for the presence of antibodies to adult T‐cell leukemia (ATL)‐associated antigens (ATLA). ATLA represent core protein(s) of ATL virus (ATLV) and ATLV‐determined polypeptides. Anti‐ATLA antibodies were found in all seven macaque species tested but not in three non‐macaque species. The frequencies of seropositive macaques ranged from 10 to 50%. In three macaque species (Japanese monkeys, rhesus monkeys, and crabeating monkeys) in which sufficient numbers of animals were studied, more females than males were anti‐ATLA positive and the antibody‐positive rate increased with age. In Japanese monkeys, over 70% were seropositive after the age of 10 years. A family study of Japanese monkeys suggested maternal transmission of ATLV or a closely related agent. These seroepidemiological features are consistent with those of Japanese in the ATL‐endemic area. Three of 10 rhesus monkeys were found to be anti‐ATLA‐positive 13 days after arrival in Japan, suggesting that they had been infected with ATLV in Indonesia where they were captured. Thus, ATLV appears to be prevalent among several macaque species as well as in man, and its pathogenetic role in these non‐human primates should be explored in relation to ATL in man.
In vitro responses of human prostate tumour cell lines to a range of antitumour agents
Authors: Metcalfe Sylvia A. , Whelan Richard D. H. , Masters John R. W. , Hill Bridget T. 
Published: February 2007

AbstractFour human prostate tumour continuous cell lines were employed for in vitro drug sensitivity testing of nine cytotoxic drugs, X‐irradiation and two hormonal preparations. Responses were assessed by measuring cell survival as judged by colony formation in agarose. A marked heterogeneity of responses was noted to many of the agents studied, including X‐irradiation. However, in terms of ID70 values for 24‐h drug exposures, comparable responses in all the cell lines tested, with values falling within a 1.4‐ to 2.9‐fold range, were noted with actinomycin D, adriamycin, m‐AMSA4, dibromodulcitol, 5‐fluorouracil, hydroxyurea and ICRF‐159. In contrast, mixed responses to cis‐platinum and methotrexate were particularly marked, with ID70 values differing by factors of 3.8 and 7.5 respectively. In addition, when higher drug concentrations of m‐AMSA, cis‐platinum, dibromodulcitol and ICRF‐159 were employed, significant deviations in the four survival curves became apparent. Continuous exposure to diethylstilboestrol and medroxyprogesterone acetate caused negligible cell kill. Due to these variable responses it was not possible to predict that any agent tested might have specific value in the treatment of prostate cancer. However, preliminary in vitro screening with such a panel of cell lines may complement in vivo studies with models of prostatic cancer and prove useful in investigations of mechanisms of drug resistance.
Effect of coordinated therapeutic assays using C. parvum, interferon and arginine butyrate on spontaneous disease and survival of AKR mice
Authors: Chany Charles , Cerutti Italina , Mace Bertrand 
Published: February 2007

AbstractAKR mice, known to develop spontaneous leukemia in almost 100% of cases, were studied throughout their lifespan. Different treatments combining a potent immune stimulator, Corynebacterium parvum (CP), with interferon (IFN) and arginine butyrate were initiated at the 15th week of life. In a preliminary series of experiments, CP (200 μg), IFN (20,000 units) and butyrate 50 mM) were employed in a well‐defined chronological order. In controls, the mean survival time (MST) was 35.17±1.67 weeks and the final survival rates was 0/50 mice for all experiments. Only CP associated with arginine butyrate significantly augmented the MST (42.5±3.66 weeks) and final survival rate (9/35 mice). In an adjusted set of experiments, reducing the IFN concentration to 10,000 and 5,000 units and that of butyrate to 6 mM greatly improved the results. The MST was substantially increased with the combinations of CP+IFN+butyrate (41.4±1.86 weeks), CP+IFN (42.73±3.29 weeks) and butyrate+IFN (41±2.34 weeks), as well as the final survival rates (8/15, 10/15 and 6/15 mice respectively). An important finding was that when CP and IFN were used separately, they were ineffective.
Patterns of tumor colony development over time in soft‐agar culture
Authors: Kirkels Wim J. , Pelgrim Olga E. , Hoogenboom Adrie M. M. , Aalders Mathilde W. , Debruyne Frans M. J. , Vooijs G. Peter , Herman Chester J. 
Published: February 2007

AbstractHuman tumors were cultured by the two‐layer soft‐agar technique and the time course of tumor colony development was evaluated during periods of up to 6 weeks in culture. All colony counting was performed with an automated tumor colony counter (Omnicon; Bausch and Lomb, Inc, Rochester, NY, USA). This instrument provided colony counts per culture plate in six size categories from >60 μm diameter colonies to > 149 μm diameter colonies. Six to 24 culture plates were used for each “growth curve”, generally 24. Control (non‐drug‐treated) cultures were obtained from 117 tumors, of which 25 also provided enough cells to allow evaluation of the time course of colony development after exposure to cytostatic agents. The development of colonies in non‐drug‐treated plates usually demonstrated a lag phase, a logarithmic growth phase to maximum colony development and a subsequent deterioration of colonies. In spite of clumps seeded into the agar, real colony growth could be recognized by frequent colony counting of culture dishes, although the temporal patterns of growth were sometimes different if pure single‐cell suspensions were compared with suspensions containing clumps from the same tumor. Drug pre‐incubation caused changes in the temporal pattern of colony growth as well as in the total number of colonies. Some cultures showed drug sensitivity when evaluated at certain time points while evaluation at later time points showed only borderline drug effect or none at all. The potential utility of tumor colony growth curves in the clinical applications of tumor colony cultures is discussed.
Enhancement by TPA of phenotypes associated with transformation in carcinogen‐treated human cells: Evidence for a selective mechanism
Authors: Siegfried Jill M. , Kaufman David G. 
Published: February 2007

AbstractThe tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) was found to increase the incidence of phenotypic alterations induced by the direct‐acting carcinogen N‐methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG) in human endometrial stromal cells. Following carcinogen treatment, changes in saturation density, γ‐glutamyl‐transpeptidase expression, morphology, and growth in selective media were enhanced in cell cultures subjected to continuous TPA exposure as compared to cultures receiving ethanol vehicle. This enhancement may have resulted, at least in part, from selection of cells altered by carcinogen, as evidenced by differences in the colony‐forming abilities of MNNG‐treated and control cultures after prolonged TPA exposure, and by differences in morphologic response to TPA challenge in these two populations.
Human lung tumour cell line adapted to grow in serum‐free medium secretes angiogenesis factor
Authors: Kumar S. , West D. , Daniel Mary , Hancock Anne , Carr T. 
Published: February 2007

AbstractA human lung tumour cell line has been grown in serum‐free medium for 12 months and during this period cells were subcultured every 4 days. The culture medium contained an angiogenesis factor which has been purified and shown to be similar to that isolated from other sources including solid tumours. This is the first report that demonstrates de novo synthesis and secretion of angiogenesis factor by a cell line adapted to grow in a serum‐free medium.
Karyotype evolution in a transformed rat cerebral endothelial cell line
Authors: Tyrkus Michael , Diglio Clement A. , Gohle Norma 
Published: February 2007

AbstractPrimary cultures of rat microvascular endothelial cells were transformed, in vitro, by exposure to Rous sarcoma virus. Transformed cells were followed and evaluated cytogenetically through numerous passages. Highly specific karyotypic changes in karyotype (both structural and numerical) were documented. These changes became established and intimately involved in further “karyotypic evolution”. The findings were reproducible, and when considered in the light of the literature suggest regular patterns of karyotypic change in rat tumors. The in vitro methodology utilized promises to be of practical value in the study of the early stages of malignancy.
A Rauscher‐virus‐induced T‐lymphocyte cell line. Induction of differentiation under influence of dimethylsulfoxide and phorbolesters
Authors: De Both N. J. , Rhijnsburger E. H. , Van Ewijk W. 
Published: February 2007

AbstractDBA/2 mice which are neonatally infected with Rauscher helper virus (R‐MuLV) develop predominantly lymphatic leukemias. From one of these lymphatic leukemias we established a permanent cell line which we named RLD (Rauscher Lymphoid DBA/2). Phenotyping of this cell line with a panel of monoclonal antibodies directed to cell‐surface determinants shows that RLD cells have T‐cell characteristics: they bind monoclonal antibodies directed to the antigens Thy‐1, T‐200 and Lyt‐1; they do not react with anti‐Lyt‐2 antibodies, nor do they react with antibodies directed to determinants on B cells or myelomonocytic cells. RLD cells show a high activity of the nuclear enzyme terminal deoxyribonucleotidyl transferase (TdT). RLD cells are able to differentiate after in vitro stimulation with 1% DMSO or with 30 nM tetra‐decanoylphorbol‐1.3‐acetate (TPA). This differentiation process is reflected by (I) changes in the 2D gel electro‐phoresis pattern of metabolically labelled proteins, (2) a decrease in TdT activity and (3) changes in the expression of cell‐surface markers. Flow cytometric analysis of stimulated RLD cells shows a strong increase in the Lyt‐1 expression. Together these data indicate that RLD cells are immature T lymphocytes which upon appropriate stimulation differentiate along the line of T helper cells.
Stable heat‐resistant clones selected from wild‐type and surface variants of B‐16 melanoma
Authors: Tao Tien‐Wen , Calderwood Stuart , Hahn George M. 
Published: February 2007

AbstractStable heat‐resistant clones were selected from wild‐type B‐16 melanoma cells and from three of their surface variants resistant to the lectins wheat‐germ agglutinin, ricin and concanavalin A. The selection procedure included three or four cycles of heating the cells in culture at 43°C for 2‐1/2 to 3‐1/2 h interspersed with growth at 37°C. The survivability of the heat‐resistant (HR) variant cells at elevated temperatures of 43°C for 160 min and 45°C for 40 min was 2–4 logs greater than that of their respective parents. This acquired property of heat resistance appeared to be a stable phenomenon, persisting in these cell lines for more than 80 generations. One HR variant line carried in tissue culture for 250 generations showed no change in the heat‐resistance characteristic. Acquisition of resistance appeared to be a gradual process with intermediate stages preceding the more pronounced degree of resistance. These newly selected HR variants join the existing surface variants of B‐16 melanoma to result in a large family of variants from the same cell lineage to make this system a powerful tool for studying the relationship between heat sensitivity, metastasis and hyperthermia treatment of cancer.
Presence of EBV‐DNA sequences in nasopharyngeal cells of individuals without IgA‐VCA antibodies
Authors: Desgranges C. , Pi G. H. , Bornkamm G. W. , Legrand C. , Zeng Y. , De‐Thé G. 
Published: February 2007

AbstractExfoliated nasopharyngeal (NP) cells from 62 normal Cantonese Chinese having IgA/VCA antibodies for more than a year and from 39 similar persons without IgA/VCA antibodies, were tested for the presence of EBV/DNA sequences by spot followed by blot hybridization tests, using the cloned internal repeat of B95‐8 viral DNA as probe. Thirteen out of 62 specimens from IgA/VCA‐positive (21%) and six out of 39 specimens (15.4%) from IgA/VCA‐negative individuals were found to contain EBV/DNA sequences. Forty‐six cases (20 IgA/VCA‐positive and 26 IgA/VCA‐negative) were followed a year later for EBV/DNA sequences and EBV serology. Half of the individuals having EBV/DNA sequences in their exfoliated NP cells in 1981 did not have detectable EBV sequences a year later, and to out of 15 negative individuals became EBV/DNA‐positive. There was no obvious correlation between EBV/DNA detectability and EBV serology. (We conclude that the best marker for NPC risk remains the increasing IgA/VCA and/or EA antibody titers.)
Expression of major histocompatibility antigens and nature of inflammatory cellular infiltrate in ovarian neoplasms
Authors: Kabawat Salim E. , Bast Robert C. , Welch William R. , Knapp Robert C. , Bhan Atul K. 
Published: February 2007

AbstractExpression of histocompatibility antigens and the intensity of inflammatory cellular infiltrate were evaluated in frozen tissue sections from 70 human ovarian tumors and six normal ovaries using monoclonal antibodies and an avidin‐biotin immunoperoxidase technique. In the normal human ovary, surface epithelial cells, mature granulosa cells and lutein cells reacted with anti‐HLA‐A, B, C (HLA) and beta2‐microglobulin antibodies but not with anti‐la (la‐like, HLA‐DR). Stromal cells and granulosa cells of the primordial follicles did not react with any of the antibodies. Among the neoplasms examined, all benign epithelial tumors, 86% of borderline an 81% of malignant epithelial tumors reacted with anti‐HLA and/or beta2‐microglobulin antibodies. HLA‐negative epithelial tumors were of serous or endometrioid types. Although la was not found in normal ovarian surface epithelium, the antigen could be detected in 44% of benign, and 43% of borderline and malignant epithelial ovarian tumors. Mono‐nuclear cellular infiltrate was generally scarce in ovarian tumors and consisted mainly of T cells. Malignant epithelial tumors contained significantly more T cells than did benign tumors. More T cells were observed in HLA‐positive ovarian tumors than in HLA‐negative neoplasms, but the difference did not achieve statistical significance. No correlation could be found between la expression and the intensity of T‐cell infiltrate. Significantly more T8 and Leu‐3a‐positive cells were found in the tumor stroma than amongst neoplastic cells. HNK‐I‐positive natural killer cells, OK‐MI‐positive macrophages and BI‐positive B lymphocytes were rarely encountered either in the tumor stroma or between adjacent tumor cells.
Karyotype evolution in B‐cell lymphoid malignancy with AN 8;14 translocation
Authors: Fukuhara Shirou , Kita Kenkichi , Nasu Kaori , Kannagi Mari , Kamezaki Tadashi , Ohno Hitoshi , Yamazowa Michihiko , Nishigori Masaru , Uchino Haruto , Yagita Masato , Matsuo Naomitsu , Yamabe Hirohiko , Tanaka Satoru 
Published: February 2007

AbstractTo evaluate the significance of karyotypic evolution of tumor cells with an 8;14 translocation [t(8;14)(q24;q32)], we examined the clinicopathologic features and immunologic phenotypes of nine Japanese patients with various types of B‐cell malignancy with the translocation. All these patients had structural rearrangements of the long arm of chromosome No. I (Iq) in a stem line or the sub‐line of tumor cells with a t(8;14). The rearrangements were composed of a translocation involving Iq with other chromosomes and a tandem duplication of Iq, and they were exclusively associated with a partial trisomy for Iq. Two patients with diffuse large‐cell lymphoma, whose tumor cells did not express surface immunoglobulins (s‐Ig), had the Iq translocation in their highly complex karyo‐types. Tumor cells from the other seven patients expressed s‐Ig and the karyotypes were relatively simple. Among these patients, the Iq translocation was found in two patients with Burkitt's lymphoma, and the Iq duplication were observed in a stem line or the sublines from four patients with Burkitt's lymphoma‐leukemia and one each with small non‐cleaved‐cell or diffuse large‐cell lymphoma. Except for one patient in the stage of IE, these patients had a poor prognosis because of the clinical conversion of extranodal metastases in the earlier disease phase. These findings are compatible with those of Western patients with a t(8;14). Therefore, tumor cells marked primarily with a t(8;14) could have “major routes” in the karyotypic evolution, for which potentials should be recognized as clinical risk factors, and the morphologic presentation and the expression of surface immunoglobulins may be associated with the process of karyotypic evolution.