bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRPV4 promotes HBV replication and capsid assembly via methylation modification of H3K4 and HBc ubiquitin
Authors: Zhang Yu , Yuan Xiaoxue , Wang Jun , Han Ming , Lu Hongping , Wang Yun , Liu Shunai , Yang Song , Xing Hui‐Chun , Cheng Jun 
Published: April 2024

AbstractHepatitis B virus (HBV) infection poses a significant burden on global public health. Unfortunately, current treatments cannot fully alleviate this burden as they have limited effect on the transcriptional activity of the tenacious covalently closed circular DNA (cccDNA) responsible for viral persistence. Consequently, the HBV life cycle should be further investigated to develop new anti‐HBV pharmaceutical targets. Our previous study discovered that the host gene TMEM203 hinders HBV replication by participating in calcium ion regulation. The involvement of intracellular calcium in HBV replication has also been confirmed. In this study, we found that transient receptor potential vanilloid 4 (TRPV4) notably enhances HBV reproduction by investigating the effects of several calcium ion‐related molecules on HBV replication. The in‐depth study showed that TRPV4 promotes hepatitis B core/capsid protein (HBc) protein stability through the ubiquitination pathway and then promotes the nucleocapsid assembly. HBc binds to cccDNA and reduces the nucleosome spacing of the cccDNA‐histones complex, which may regulate HBV transcription by altering the nucleosome arrangement of the HBV genome. Moreover, our results showed that TRPV4 promotes cccDNA‐dependent transcription by accelerating the methylation modification of H3K4. In conclusion, TRPV4 could interact with HBV core protein and regulate HBV during transcription and replication. These data suggest that TRPV4 exerts multifaceted HBV‐related synergistic factors and may serve as a therapeutic target for CHB.
The use of human iPSC‐derived alveolar organoids to explore SARS‐CoV‐2 variant infections and host responses
Authors: Gandikota Chaitanya , Vaddadi Kishore , Sivasami Pulavendran , Huang Chaoqun , Liang Yurong , Pushparaj Samuel , Deng Xufang , Channappanavar Rudragouda , Metcalf Jordan P. , Liu Lin 
Published: April 2024

AbstractSevere acute respiratory syndrome‐related coronavirus 2 (SARS‐CoV‐2) primarily targets the respiratory system. Physiologically relevant human lung models are indispensable to investigate virus‐induced host response and disease pathogenesis. In this study, we generated human induced pluripotent stem cell (iPSC)‐derived alveolar organoids (AOs) using an established protocol that recapitulates the sequential steps of in vivo lung development. AOs express alveolar epithelial type II cell protein markers including pro‐surfactant protein C and ATP binding cassette subfamily A member 3. Compared to primary human alveolar type II cells, AOs expressed higher mRNA levels of SARS‐CoV‐2 entry factors, angiotensin‐converting enzyme 2 (ACE2), asialoglycoprotein receptor 1 (ASGR1) and basigin (CD147). Considering the localization of ACE2 on the apical side in AOs, we used three AO models, apical‐in, sheared and apical‐out for SARS‐CoV‐2 infection. All three models of AOs were robustly infected with the SARS‐CoV‐2 irrespective of ACE2 accessibility. Antibody blocking experiment revealed that ASGR1 was the main receptor for SARS‐CoV2 entry from the basolateral in apical‐in AOs. AOs supported the replication of SARS‐CoV‐2 variants WA1, Alpha, Beta, Delta, and Zeta and Omicron to a variable degree with WA1 being the highest and Omicron being the least. Transcriptomic profiling of infected AOs revealed the induction of inflammatory and interferon‐related pathways with NF‐κB signaling being the predominant host response. In summary, iPSC‐derived AOs can serve as excellent human lung models to investigate infection of SARS‐CoV‐2 variants and host responses from both apical and basolateral sides.
Global burden of vaccine‐associated multiple sclerosis, 1967–2022: A comprehensive analysis of the international pharmacovigilance database
Authors: Woo Ho Geol , Kim Hyeon Jin , Park Jaeyu , Lee Jinseok , Lee Hayeon , Kim Min Seo , Koyanagi Ai , Smith Lee , Rahmati Masoud , Yeo Seung Geun , Yon Dong Keon 
Published: April 2024

AbstractVaccine‐associated multiple sclerosis (MS) is rare, with insufficient evidence from case reports. Given the scarcity of large‐scale data investigating the association between vaccine administration and adverse events, we investigated the global burden of vaccine‐associated MS and potential related vaccines from 1967 to 2022. Reports on vaccine‐associated MS between 1967 and 2022 were obtained from the World Health Organization International Pharmacovigilance Database (total number of reports = 120 715 116). We evaluated global reports, reporting odds ratio (ROR), and information components (IC) to investigate associations between 19 vaccines and vaccine‐associated MS across 156 countries and territories. We identified 8288 reports of vaccine‐associated MS among 132 980 cases of all‐cause MS. The cumulative number of reports on vaccine‐associated MS gradually increased over time, with a substantial increase after 2020, owing to COVID‐19 mRNA vaccine‐associated MS. Vaccine‐associated MS develops more frequently in males and adolescents. Nine vaccines were significantly associated with higher MS reporting, and the highest disproportional associations were observed for hepatitis B vaccines (ROR 19.82; IC025 4.18), followed by encephalitis (ROR 7.42; IC025 2.59), hepatitis A (ROR 4.46; IC025 1.95), and papillomavirus vaccines (ROR 4.45; IC025 2.01). Additionally, MS showed a significantly disproportionate signal for COVID‐19 mRNA vaccines (ROR 1.55; IC025 0.52). Fatal clinical outcomes were reported in only 0.3% (21/8288) of all cases of vaccine‐associated MS. Although various vaccines are potentially associated with increased risk of MS, we should be cautious about the increased risk of MS following vaccination, particularly hepatitis B and COVID‐19 mRNA vaccines, and should consider the risk factors associated with vaccine‐associated MS.
Reevaluating the FDA's warning against the use of probiotics in preterm neonates: A societal statement by ESPGHAN and EFCNI
Authors: van den Akker Chris H. P. , Embleton Nicholas D. , Lapillonne Alexandre , Mihatsch Walter A. , Salvatore Silva , Canani Roberto B. , Dinleyici Ener C. , Domellöf Magnus , Guarino Alfredo , Gutiérrez‐Castrellón Pedro , Hojsak Iva , Indrio Flavia , Mosca Alexis , Orel Rok , van Goudoever Johannes (Hans) B. , Weizman Zvi , Mader Silke , Zimmermann Luc J. I. , Shamir Raanan , Vandenplas Yvan , Szajewska Hania 
Published: April 2024

AbstractThe recent advisory issued by the United States Food and Drug Administration, cautioning against the routine administration of probiotics in preterm neonates, has sparked a lively debate within the scientific community. This commentary presents a perspective from members of the Special Interest Group on Gut Microbiota and Modifications within the European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) and other authors who contributed to the ESPGHAN position paper on probiotics for preterm infants, as well as representatives from the European Foundation for the Care of Newborn Infants. We advocate for a more nuanced and supportive approach to the use of certain probiotics in this vulnerable population, balancing the demonstrated benefits and risks.
Odevixibat as an adjunctive treatment for refractory pruritus in rare variants of cholestatic liver disease
Authors: Goel Akshat , Tucker Bethany , Casale Lorena Soler , Grammatikopoulos Tassos 
Published: April 2024

AbstractObjectivesOdevixibat, a reversible ileal bile acid transport inhibitor, has been shown to reduce serum bile acids (sBA) and pruritus mostly in children with progressive familial intrahepatic cholestasis (PFIC) 1 and 2 in clinical trials and case reports. There are currently no published case reports or series describing its use in rare variants of cholestatic liver disease.MethodsWe describe three children with progressive cholestatic liver disease who developed refractory pruritus, who had a genotypic diagnosis of AKR1D1, ABCB4 variant, and PKHD1 and PKHD2 variants; all being variants of unknown significance as per the American College of Medical Genetics and Genomics guidelines.ResultsOn Odevixibat there was a significant improvement in sBA (absolute change from baseline: −196 and −393 μmol/L) and pruritus in two children with heterozygous AKR1D1 and ABCB4 mutations. The child with ABCB4 variants was found to have features of sclerosing cholangitis along with a diagnosis of Crohn's disease, which represents the first reported usage of Odevixibat in such a case with good response. There was some reported improvement in the third child with PKHD1 and PKHD2 variants; however, we hypothesize that no sustained improvement could be due to severe and progressive nature of the disease. There were no side effects reported and it was well tolerated in all.ConclusionWe suggest that Odevixibat may be used as an adjunctive drug in refractory pruritus and could be started early in the course of disease if clinically and phenotypically indicated.
On the concept of the regional religious brand: The veneration and pilgrimage of Saint John Vladimir and new pathways for religious tourism
Authors: Avdia Blerta , Bici Alma , Giakoumis Konstantinos 
Published: April 2024

AbstractBuilding on the concept of regional branding, the paper seeks to pave new ground by delving into the novel concept of a ‘regional religious brand.’ As a new term in religious tourism, the ‘regional religious brand’ defines the function of distinguishing a spiritual value offered in a region as a concept and reality, containing a name, an iconography, a set of images, a pilgrimage, narratives, a slogan, visions, and symbols. After arguing that the territory of the former Archdiocese of Ohrid still qualifies as a region, we engage in one of the region's spiritual symbols, the veneration and pilgrimage of Saint John Vladimir and suggest a concrete pilgrimage route stemming from this regional religious brand.
Benzoylation of Iota Carrageenan: Development of a Stable, Conductive, and Hydrophobic Drug Carrier with Reduced Toxicity and Improved Gel‐Forming Ability
Authors: Bhatt Akanksha , Kailkhura Shashank , Purohit Priyank 
Published: April 2024

AbstractIota carrageenan is characterized as a hydrocolloid, with the limitations of hygroscopicity and hydrophilicity. Here, each of these factors is addressed to enhance the stability, and hydrophobicity through the protection of highly reactive primary alcohol and the formation of stable, improved hydrophobic, and conductive gel for the loading of lipophilic drugs. The functionalized polymer is evaluated through the microscopic and scanning electron microscopy (SEM) image of the compounds, which confirm the confined nature of the functionalized polymer. Moreover, the values changed from compound 1 (iota carrageenan) to compound 2 (benzoyl liked iota carrageenan) included Log P (partition coefficient) from −0.97 to 0.90, calculated partition coefficient (cLog P) from −2.50 to 0.12, and total polar surface area (TPSA) from 214.1 to 208.57, which strongly suggest the enhanced hydrophobic character of functionalized polymers. The enhancement is further concluded by the loading of the lipophilic drug inside the core of functionalized polymer‐based gel. The enhanced loading of the drug clearly shows the improved hydrophobicity (56–86%) of the gel core of the functionalized compounds. In summary, a polymer with enhanced stability, hydrophobicity, conductivity, and capacity to make a more stable gel, with enhanced drug‐loading capability of water‐insoluble drugs, will be the best way to resolve the low bioavailability issue of the water‐insoluble drug.
Design of miniaturized 2.5D frequency selective surface for X‐ and Ku‐band applications
Authors: Suganya Anandan , Natarajan Rajesh 
Published: April 2024

AbstractThis paper presents the design of 2.5D frequency selective surface (FSS) for X and Ku band applications. Proposed FSS's unit cell is constructed with two independent slitted rings on both side of the FR4 substrate and is connected with metallic vias. The FSS offers a wide stop band performance from 6.26 to 18 GHz, with a fractional bandwidth of 96.7% measured at S21 < −10 dB and has a low insertion loss (IL) of 0.018 dB. The footprint of the FSS is only about 0.11 with the shielding effectiveness of 83.365 dB, and the transverse electric and transverse magnetic mode performances are stable up to 70 degrees. Prototype FSS is fabricated and measured for its performance validation. The performance validation is carried out using applied wave research and the equivalent circuit model tools.
Facile preparation of thermally conductive fiber film by self‐assembling interconnected boron nitride nanosheets for effective thermal interface materials
Authors: Ye Bin , Li Ling , Dai Kun , Xie Zuoxiang , Zhou Qinhua , Dong Yuyang , Zheng Qingbin , Park Soo‐Jin , Zhang Yinhang 
Published: April 2024

AbstractBoron nitride (BN) has garnered significant attention for its potential in developing thermally conductive materials owing to its inherent insulating properties and high thermal conductivity. However, achieving high thermal conductivity in bulk materials or 3D structures constructed by BN sheets has remained challenging. In this study, we present a novel approach to design a high‐quality BN fiber using xanthan gum (XG) as a skeleton, aiming to facilitate heat dissipation in integrated circuits. The resulting thermally conductive film, with orderly and compactly arranged BN sheets in the XG skeleton, exhibits a remarkable in‐plane thermal conductivity of 8.26 Wm−1 K−1 and an out‐of‐plane thermal conductivity of 1.35 Wm−1 K−1. This film efficiently enables fast heat dissipation for LED chips and thermoelectric generators. Finite element simulation further supports the efficient heat transfer capacity of the BN fiber films.Highlights
A high‐quality BN fiber was prepared by a facile approach.
The BN fiber film exhibited high thermal conductivity.
This film enables fast heat dissipation in practical applications.
Effect of the molar mass of polyimide based on pyromellitic dianhydride and 4,4′‐oxydianiline on dielectric and mechanical properties of nonwoven oriented polyimide materials
Authors: Kamalov Almaz , Vaganov Gleb , Simonova Maria , Kraft Vadim , Nesterova Anna , Saprykina Natalia , Romasheva Margarita , Filippov Alexander , Yudin Vladimir 
Published: April 2024

AbstractThis work is dedicated to the development and investigation of the properties of low‐dielectric‐constant nonwoven materials obtained by electrospinning from a polyimide‐based polymer, PMDA‐ODA, which is composed of pyromellitic dianhydride (PMDA) and 4,4′‐oxydianiline (ODA). The influence of the molar mass of PMDA‐ODA on the structural, mechanical, and dielectric properties of the resulting samples has been investigated. The molar mass of the polyimide has been determined through light scattering and viscosity measurements of polyamic acid solutions. As the molar mass of PMDA‐ODA increases from 13.400 to 49.300 g/mol, the glass transition temperature of the produced nonwoven samples increases, and their tensile mechanical properties are improved. The dielectric constant of PMDA‐ODA nonwoven polyimide materials varies from 1.55 to 1.91 at a temperature of 30°C in a frequency range from 1 Hz to 1 MHz, depending on the molar mass of the polymer used.
High levels of genetic variation and differentiation in wild tropical gourds provide a novel resource for cucurbit crop improvement
Authors: Castellanos‐Morales Gabriela , Aguirre‐Dugua Xitlali , Scheinvar Enrique , Gasca‐Pineda Jaime , Sánchez‐de la Vega Guillermo , Aguirre‐Planter Erika , Lira‐Saade Rafael , Eguiarte Luis E. 
Published: April 2024

Social Impact StatementThe genetic variation of crop wild relatives will be key for our survival, as environmental change represents an increasing global threat for agriculture and food security. Cucurbita lundelliana and Cucurbita okeechobeensis subsp. martinezii are wild relatives of cultivated squashes and pumpkins that could be used for crop improvement. A genomic approach was used to characterize genetic resources in these taxa, that is, to identify candidate sites in the genome involved in responses to abiotic stress, and to understand the roles of gene flow and environmental differentiation in their divergence. This study highlights the importance of conserving these two species as phytogenetic resources for crop improvement.Summary

Crop wild relatives (CWR) are reservoirs of genetic diversity and they are important for the maintenance of crop evolutionary potential. Mexico is the centre of domestication and diversity for many CWR. The genus Cucurbita originated in America, where at least six independent domestication events took place. Nonetheless, Cucurbita CWR have been seldom studied. In the present study, we test the role of incomplete lineage sorting (ILS) and secondary contact in the divergence of C. okeechobeensis subsp. martinezii and C. lundelliana. Additionally, we seek to understand the role of environmental differentiation in their divergence.
We obtained 1,638 single nucleotide polymorphisms (SNPs) for 107 individuals from the most recently diverged wild species in this genus, encompassing 25 localities of C. lundelliana and nine localities of C. okeechobeensis subsp. martinezii in Mexico.
We found higher genetic variation in C. lundelliana (HE = 0.227) than in C. okeechobeensis subsp. martinezii (HE = 0.187). Each taxon constitutes a well‐differentiated genetic group, with an area of introgression in Pantanos de Centla. We found candidate loci associated to hydric and thermal stress, which could be valuable for crop improvement. Our study supported a scenario of ILS followed by secondary contact, where divergence was probably driven by Pleistocene climate change.
These CWR represent important phytogenetic resources for crop improvement given their high levels of genetic variation and differentiation and their SNPs associated to different climatic variables.
Ten‐year functional and oncological outcomes of a prospective randomized controlled trial comparing laparoscopic versus robot‐assisted radical prostatectomy
Authors: Checcucci Enrico , De Cillis Sabrina , Alladio Eugenio , Piramide Federico , Volpi Gabriele , Granato Stefano , Zamengo Davide , Bignante Gabriele , Amparore Daniele , Piana Alberto , Manfredi Matteo , Vallariello Edoardo , Stura Ilaria , Di Dio Michele , Autorino Riccardo , Porpiglia Francesco , Fiori Cristian 
Published: April 2024

AbstractBackgroundAmong prostate cancer (PCa) treatment options, mini‐invasive surgical approaches have gained a wide diffusion in the last decades. The aim of this study was to present oncological, functional, and quality of life data after 10 years of follow‐up of a prospective randomized controlled trial (RCT) (ISRCTN11552140) comparing robot‐assisted radical prostatectomy (RARP) versus laparoscopic radical prostatectomy (LRP) for the treatment of PCa.MethodsPatients with localized PCa were randomized to undergo LRP or RARP between January 2010 and January 2011. Functional (continence and potency) and oncological (prostate‐specific antigen, biochemical recurrence [BCR] and BCR‐free survival [BCRFS]) variables were evaluated. BCRFS curves were estimated by the Kaplan–Meier method and compared using the log‐rank test. Machine learning partial least square‐discriminant analysis (PLS‐DA) was used to identify the variables characterizing more the patients who underwent RARP or LRP.ResultsSeventy‐five of the originally enrolled 120 patients remained on follow‐up for 10 years; 40 (53%) underwent RARP and 35 (47%) LRP. Continence and potency recovery rates did not show significant differences (p = 0.068 and p = 0.56, respectively), despite a Δ12% for continence and Δ8% for potency in favor of the robotic approach. However, the quality of continence (in terms of International Consultation on Incontinence Questionnaire‐Short Form [ICIQ‐SF] score) and erection (in terms of International Index of Erectile Function‐5 [IIEF‐5] score) was significantly better after 10 years in the robotic group (p = 0.02 and p < 0.001). PLS‐DA revealed that LRP was characterized by the worst functional‐related outcomes analyzing the entire follow‐up period. Four (10%) and six (17%) patients experienced BCR in RARP and LRP groups, respectively (p = 0.36), with an overall 10‐year BCR‐free survival of 88% and 78% (p = 0.16).ConclusionsComparable continence and potency rates were observed between RARP and LRP after a 10‐year follow‐up. However, the RARP group exhibited superior totally dry rate and erection quality. No difference in terms of oncological outcomes was found.
Engineering Material Properties of Transcription Factor Condensates to Control Gene Expression in Mammalian Cells and Mice
Authors: Fischer Alexandra A.M. , Robertson Hanah B. , Kong Deqiang , Grimm Merlin M. , Grether Jakob , Groth Johanna , Baltes Carsten , Fliegauf Manfred , Lautenschläger Franziska , Grimbacher Bodo , Ye Haifeng , Helms Volkhard , Weber Wilfried 
Published: April 2024

AbstractPhase separation of biomolecules into condensates is a key mechanism in the spatiotemporal organization of biochemical processes in cells. However, the impact of the material properties of biomolecular condensates on important processes, such as the control of gene expression, remains largely elusive. Here, the material properties of optogenetically induced transcription factor condensates are systematically tuned, and probed for their impact on the activation of target promoters. It is demonstrated that transcription factors in rather liquid condensates correlate with increased gene expression levels, whereas stiffer transcription factor condensates correlate with the opposite effect, reduced activation of gene expression. The broad nature of these findings is demonstrated in mammalian cells and mice, as well as by using different synthetic and natural transcription factors. These effects are observed for both transgenic and cell‐endogenous promoters. The findings provide a novel materials‐based layer in the control of gene expression, which opens novel opportunities in optogenetic engineering and synthetic biology.
Facile Magnetite Coating for Stable High‐Voltage Cycling of Nickel‐Rich Cathodes in Conventional Liquid and All‐Solid‐State Lithium‐Ion Batteries
Authors: Mahajani Varad , Bhimani Kevin , Lakhnot Aniruddha Singh , Panchal Reena , Anjan Apurva , Manoj Rohit M. , Koratkar Nikhil 
Published: April 2024

AbstractThe instability of Nickel (Ni)‐rich cathodes at high voltage is a critical bottleneck toward developing superior lithium‐ion batteries. This instability is driven by cathode‐electrolyte side reactions, causing rapid degradation, and compromising the overall cycle life. In this study, a protective coating using dispersed “magnetite (FeO.Fe2O3)” nanoparticles is used to uniformly decorate the surface of LiNi0.8Co0.1Mn0.1O2 (NMC 811) microparticles. The modified cathode delivers significant improvement in electrochemical performance at high voltage (≈4.6 V) by suppressing deleterious electrode–electrolyte interactions. A notably higher cycle stability, rate performance, and overall energy density is realized for the coated cathode in a conventional liquid electrolyte battery. When deployed in pellet‐stacked solid‐state cells with Li6PS5Cl as the electrolyte, the magnetite‐coated NMC 811 showed strikingly superior cycling stability than its uncoated counterpart, proving the versatility of the chemistry. The facile surfactant‐assisted coating process developed in this work, in conjunction with the affordability, abundance, and nontoxic nature of magnetite makes this a promising approach to realize commercially viable high voltage Ni‐rich cathodes that exhibit stable performance in liquid as well as solid‐state lithium‐ion batteries.