bluuebunny/crossref_metadata_2025_split huggingface dataset:
脳死下臓器移植経験施設の施設特徴とその地域性の評価：多施設後ろ向き観察研究(Evaluation of institutional and regional characteristics of facilities with experience in organ transplantation under brain death: A multi–center, retrospective observational study)
Authors: 主基 出内 
Published: April 2024

要旨【目的】2021年に日本救急医学会の脳死・臓器組織移植に関する委員会により作成された脳死判定を目的とした転院搬送についての意識調査に関する委員会報告では，救命救急センターでも3分の1の施設が過去に臓器提供経験がないと判明した。本研究は臓器提供に至る施設の特徴や地域間格差の原因を探索することを目的とした。【対象】上記の意識調査（救命救急センター 295施設を対象，220施設より回答）を二次解析した。都道府県別腎臓提供数は過去臓器提供を行った全施設を対象とし日本臓器移植ネットワークの情報を用いた。【結果】救命救急センターにおける脳死下臓器移植の有無と，救命救急センター充実段階評価における「救急での人生の最終段階における医療の整備」が有意に関連していた（71.1% vs 53.8%, p=0.04）。また，人口10万あたり腎臓提供数が少ない地域では「救急での人生の最終段階における医療の整備」を行っている割合が少なかった（Q1+Q2+Q3 vs Q4; 85.6% vs 66.7%, p=0.01）。【結語】救命救急センターでの人生の最終段階における医療体制整備は地域・病院間で格差があり，臓器提供件数が少ない病院や都道府県で体制の整備が遅れている傾向が示された。人生の最終段階における医療体制整備を医療計画に基づいて支援することは臓器提供機会の適正化に寄与する可能性が示唆された。
Promoting effects of aluminum addition on chlorophyll biosynthesis and growth of two cultured iron‐limited marine diatoms
Authors: Zhou Linbin , Liu Fengjie , Achterberg Eric P. , Engel Anja , Campbell Peter G.C. , Fortin Claude , Huang Liangmin , Tan Yehui 
Published: April 2024

AbstractAluminum (Al) may play a role in the ocean's capacity for absorbing atmospheric CO2 via influencing carbon fixation, export, and sequestration. Aluminum fertilization, especially in iron (Fe)‐limited high‐nutrient, low‐chlorophyll ocean regions, has been proposed as a potential CO2 removal strategy to mitigate global warming. However, how Al addition would influence the solubility and bioavailability of Fe as well as the physiology of Fe‐limited phytoplankton has not yet been examined. Here, we show that Al addition (20 and 100 nM) had little influence on the Fe solubility in surface seawater and decreased the Fe bio‐uptake by 11–22% in Fe‐limited diatom Thalassiosira weissflogii in Fe‐buffered media. On the other hand, the Al addition significantly increased the rate of chlorophyll biosynthesis by 45–60% for Fe‐limited T. weissflogii and 81–102% for Fe‐limited Thalassiosira pseudonana, as well as their cell size, cellular chlorophyll content, photosynthetic quantum efficiency (Fv/Fm) and growth rate. Under Fe‐sufficient conditions, the Al addition still led to an increased growth rate, though the beneficial effects of Al addition on chlorophyll biosynthesis were no longer apparent. These results suggest that Al may facilitate chlorophyll biosynthesis and benefit the photosynthetic efficiency and growth of Fe‐limited diatoms. We speculate that Al addition may enhance intracellular Fe use efficiency for chlorophyll biosynthesis by facilitating the superoxide‐mediated intracellular reduction of Fe(III) to Fe(II). Our study provides new evidence and support for the iron–aluminum hypothesis.
Social media marketing activities, customer engagement, and customer stickiness: A longitudinal investigation
Authors: So Kevin Kam Fung , Li Jing , King Ceridwyn , Hollebeek Linda D. 
Published: April 2024

AbstractAlthough customer engagement has been deemed critical for optimizing social media marketing performance, prior research has mainly examined customer engagement through cross‐sectional designs, thus limiting insight into its evolving dynamics over time. To address this gap, we adopt uses‐and‐gratifications theory to explore the associations among customers' evaluations of a firm's social media marketing activities, customer engagement, and customer stickiness on a brand's social media page at three points in the post‐purchase stage of the consumption journey. Cross‐lagged autoregressive panel model analyses suggest that, first, customers' evaluations of firms' social media marketing activities, customer engagement, and customer stickiness fluctuate. Second, customer engagement has cross‐lagged longitudinal effects on stickiness over time; the same applies to the role of stickiness in customers' evaluations of the firm's social media marketing activities. However, no cross‐lagged effects arise from (a) firm‐based social media marketing activities to customer stickiness or (b) stickiness to engagement. These findings contribute to the customer engagement literature by describing the dynamics of such engagement and its interrelationships with other key constructs across time.
Exploring spatial and temporal resilience in socio‐ecological systems: Evidence from sacred forests in Epirus, Greece
Authors: Marini Govigli Valentino , Healey John R. , Wong Jennifer L. G. , Stara Kalliopi , Tsiakiris Rigas , Halley John M. 
Published: April 2024

Abstract

Socio‐ecological resilience is the capacity of a system to adapt to changing ecological and social disturbances. Its assessment is extremely important to integrate long‐term management of ecological and social features of natural ecosystems. This is especially true for Sacred Natural Sites, such as sacred forests and groves, where it can reveal the influence of social processes in ecosystem recovery or degradation.

Using tree ages determined through dendrochronology and tree population size‐class distributions collected in five sacred forests in Epirus (NW Greece), we explore spatial and temporal dynamics of resilience in a socio‐ecological system, identifying which cultural and social elements characterize resilience in space and time.

Our main results show that over past centuries sacred forests in Epirus underwent periods of varying tree establishment rate, depending on the intensity of human activities and historical disturbance events.

We also identified strong evidence of the role of the social component (i.e. the church and associated cultural praxis) in determining the spatial extent of the forests' current recovery phase, and thus the overall resilience of the system.

Policy implications. Appreciation of the ways sacred forests' ecological resilience is linked to changing socio‐cultural praxis over both temporal and spatial scales is crucial for guiding conservation and restoration strategies. We argue that greater attention should be paid to the role of the social component of socio‐ecological systems and specifically for sacred natural sites that provide both a nucleus of established forest habitat and the conditions necessary for forest recovery and restoration.

Read the free Plain Language Summary for this article on the Journal blog.
Follow‐up results regarding fixation of contralateral testis after testicular torsion in children
Authors: Yu Chengjun , Wei Yi , Shi Qinlin , Wen Sheng , Lin Tao , He Dawei , Wei Guanghui , Wu Shengde 
Published: April 2024

AbstractThe aim is to investigate the possible advantages and disadvantages of contralateral testicular fixation (CTF) for boys with testicular torsion (TT) by long‐term clinical follow‐up results. A retrospective cohort study was conducted on TT boys at Children’s Hospital of Chongqing Medical University from September 2005 to December 2020. The baseline characteristics, intraoperative findings, and clinical follow‐up results were compared between TT patients underwent orchiopexy with or without CTF, and orchiectomy with or without CTF, respectively. The χ2 test and the Fisher’s Exact test were used to compare dichotomous outcomes, and the Mann‐Whitney U test was used to compare continuous outcomes without normal distribution. A total of 140 cases were included in this study. The bell clapper deformity was found in 30.4% of all torsed testes underwent orchiopexy, 16.4% during contralateral testicular exploration, and only 10.9% in bilateral sides. Contralateral testicular volume, adverse events, and paternity rates were comparable between patients underwent orchiopexy with or without CTF, and no subsequent contralateral TT observed during follow‐up. However, those patients underwent orchiectomy without CTF had more incidence of scrotal discomfort than those had orchiectomy with CTF (P = 0.027). In addition, one boy suffered subsequent contralateral TT after orchiectomy without CTF. The incidence of bell clapper deformity was lower in this research than that of literature reports. CTF would not induce testicular volume loss, atrophy or scrotal pain. Contralateral testicular discomfort and even subsequent TT could be more easily observed during follow‐up in patients without CTF. We still recommend routine surgical fixation of contralateral testicle during surgery.
The shape of things to come
Authors: Kliner Dahv , Kingsbury Alex 
Published: April 2024

AbstractAll‐fiber beam shaping is revolutionizing laser‐based manufacturing. This capability is a commercially accessible reality in cutting, welding, and additive manufacturing tools released by leading integrators worldwide. These advanced tools increase productivity and part quality and introduce entirely new production capabilities, driving the displacement of legacy lasers and non‐laser technologies in existing applications and spurring the development of new markets.
Laser sources: global market and trends
Authors: Robin Thierry , Cochard Jacques , Moratis Kimon 
Published: April 2024

AbstractThe laser industry experienced significant growth in 2021 and 2022, reaching a value of 19.3 billion US dollars in 2022 after two challenging years in 2019 and 2020. Despite the global effects of the war in Ukraine, the ongoing trade war between the United States and China, and the aftermath of the Covid‐19 pandemic, this industry showed remarkable resilience. The demand for laser sources in both industry and consumer products has significantly accelerated their sales. If this trend is not affected by any destabilizing factors, the global laser sources market is expected to reach a total value of 26.8 billion US dollars by 2027, with a growth rate of 6.8 %.
scRNA‐seq determines the characteristics of T cell marker genes to predict the prognosis of esophageal cancer
Authors: Meng Fang , Hu Jiaru , Qiang Jinhu , Shi Qifeng , Ji Jing , Li Zhenyu , Li Lu , Lv Lei , Zhang Shiqiang 
Published: April 2024

AbstractEsophageal cancer (EC) mainly includes two histological subtypes, esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma, which is of high morbidity and mortality. With the continuous development of medical technology, the treatment of EC has been greatly improved, but its prognosis is still unfavorable. Recent single‐cell RNA‐sequencing (scRNA‐seq) is expected to bring breakthroughs in the treatment of EC. First, we identified T cell marker genes and generated signature by analyzing scRNA‐seq data from Expression Omnibus (GEO) database and TCGA database. Then, an immune prognostic model was constructed using the least absolute shrinkage and selection operator. We found that the survival rate of ESCC varied significantly among the low‐ and high‐risk groups. Two genes from T cell signature, DCPS and CYB5R3, were expressed in ESCC cells. Collectively, our study proposed a novel prognostic signature for ESCC patients based on T cell marker genes.
Dispersion evaluation of hydrogen sulfide inclusion in a cryogenic distillation pilot plant
Authors: Mohd Shukor Mohd Syazwan , Omar Rozita , Abdul Hamid Mohamad Rezi , Harun Mohd Razif , Said Mohamad Syazarudin Md 
Published: April 2024

AbstractA risk analysis simulation was completed for a cryogenic distillation (Cryo‐D) pilot operation processing a natural gas feed stream containing H2S. This study placed significant H2S concentration inside the Cryo‐D and evaluated its dispersion after a simulated leak within the pilot plant. Sensitivity analysis using an in‐house process simulation identified the operating conditions that caused the highest surroundings H2S concentration. A computational fluid dynamic (CFD) dispersion simulation assessed H2S leakage at the bottom column section and its effects on operators. A case with 60 bar column pressure, −30°C inlet temperature and 10% H2S input gas had the highest mass flow rate of 4.74 kg/h H2S through a leak. Leaked H2S covers 50% of the building within 60 s. Within 120 s, H2S accumulates toward the column bottom. At 120 s, most operators are exposed to above 1000 ppm H2S, which may cause fatalities instantly. Recommendations include enhancing the ventilation of the building by installing ventilation near the base of the cryogenic column in order to lessen the exposure concentration. In conclusion, H2S leakage from an indoor Cryo‐D pilot plant can cause catastrophic consequences due to its rapid dispersion and accumulation throughout the building.
Navigating the complexities of student understanding: Exploring the coherency of students' conceptions about the greenhouse effect
Authors: Schubatzky Thomas , Haagen‐Schützenhöfer Claudia , Wackermann Rainer , Wöhlke Carina , Wildbichler Sarah 
Published: April 2024

AbstractThe greenhouse effect is a complex scientific phenomenon that plays a crucial role in understanding climate change. Grasping students' understanding of this phenomenon on the content‐specific level but also how students' conceptions are organized is vital for effective climate change education. This study addresses both levels and delves into the relationship between students' frameworks and knowledge pieces of the greenhouse effect through the analysis of multiple‐choice questions, employing Bayesian correlations and multiple logistic regression. We thereby focus on specific types of conceptualizations of the greenhouse effect that have been identified in previous research and furthermore investigate the coherency of them. To do so, we analyzed answers of N = 604 grade 11 students in Austria and Germany and interpreted them from different theoretical perspectives. The findings showed that students hold various ideas about the greenhouse effect that are only seldom coherent, in particular when it comes to adequate ideas about the greenhouse effect. However, especially for a reflection‐based framework of the greenhouse effect, our results demonstrate that students' conceptions show some form of coherency. We argue that our results can inform the development of effective teaching strategies that address students' existing knowledge and alternative conceptions. In terms of practical implications, the findings suggest that teaching strategies should provide opportunities for students to integrate their knowledge pieces into a more coherent understanding of the greenhouse effect. The study highlights the need for further investigation into the relationship between knowledge pieces and frameworks not only for the greenhouse effect, but for science education in general.
The In Vivo Biological Fate of Protein Corona: A Comparative PET Study of the Fate of Soft and Hard Protein Corona in Healthy Animal Models
Authors: Villacorta Angel Martinez , Mielcarek Angelika , Martinez María Gómez , Jorge Helena , Henschke Agata , Coy Emerson , Gomez‐Vallejo Vanessa , Llop Jordi , Moya Sergio E. 
Published: April 2024

AbstractRadiolabeling and nuclear imaging techniques are used to investigate the biodistribution patterns of the soft and hard protein corona around poly (lactic‐co‐glycolic acid) nanoparticles (PLGA NPs) after administration to healthy mice. Soft and hard protein coronas of 131I‐labeled BSA or 131I‐labeled serum are formed on PLGA NPs functionalized with either polyehtylenimine (PEI) or bovine serum albumin (BSA). The exchangeability of hard and soft corona is assessed in vitro by gamma counting exposing PLGA NPs with corona to non‐labeled BSA, serum, or simulated body fluid. PEI PLGA NPs form larger and more stable coronas than BSA PLGA NPs. Soft coronas are more exchangeable than hard ones. The in vivo fate of PEI PLGA NPs coated with preformed 18F‐labeled BSA hard and soft coronas is assessed by positron emission tomography (PET) following intravenous administration. While the soft corona shows a biodistribution similar to free 18F BSA with high activity in blood and kidney, the hard corona follows patterns characteristic of nanoparticles, accumulating in the lungs, liver, and spleen. These results show that in vivo fates of soft and hard corona are different, and that soft corona is more easily exchanged with proteins from the body, while hard corona is largely retained on the nanoparticle surface.
Minimizing Voltage Losses via Synergistically Reducing Hetero‐Interface Energy Offset for High Efficiency Perovskite Solar Cells
Authors: Wang Xinxin , Huang Hao , Wang Min , Lan Zhineng , Yang Yingying , Cui Peng , Du Shuxian , Yan Luyao , Zhang Qiang , Qu Shujie , Zhao Zhiguo , Li Meicheng 
Published: April 2024

AbstractThe open circuit voltage (VOC) losses at multiple interfaces within perovskite solar cells (PSCs) limit the improvements in power conversion efficiency (PCE). Herein, a tailored strategy is proposed to reduce the energy offset at both hetero‐interfaces within PSCs to decrease the VOC losses. For the interface of perovskite and electron transport layer where exists a mass of defects, it uses the pyromellitic acid to serve as a molecular bridge, which reduces non‐radiative recombination and energy level offset. For the interface of perovskite and hole transport layer, which includes a passivator of PEAI, the detrimental effect (negative shift of work function) of PEAI passivation and optimizing the interface energy level alignment are neutralized by incorporating (2‐(4‐(bis(4‐methoxyphenyl)amino)phenyl)‐1‐cyanovinyl)phosphonic acid. Owing to synergistically reduced hetero‐interface energy offset, the PSCs achieve a PCE of 25.13%, and the VOC is increased from 1.134 to 1.174 V. In addition, the resulting PSCs possess enhanced stability, the unencapsulated PSCs can maintain ≈96% and ≈97% of their initial PCE after 2000 h of aging under ambient conditions and 210 h under operation conditions.
Schnelles und effizientes Genom-Editieren in Aureobasidium
Authors: Zimmermann Christian 
Published: April 2024

AbstractThe fungus Aureobasidium pullulans is used in the industry to produce pullulan and polymalate and possesses some outstanding properties such as polyextremotolerance and an unusual life cycle. This makes A. pullulans an interesting study object, for which we need an efficient genome editing method. This article describes how CRISPR/Cas can be used for this purpose. In particular, the usage of in vitro assembled ribonucleoproteins proved to be a fast and efficient method for genome editing.
Organisation von Gehirnzellen auf nanostrukturierten Materialien
Authors: Abend Alice , Jahnke Heinz-Georg , Zink Mareike 
Published: April 2024

AbstractBrain-on-a-chip devices offer great potential to study function and disease of the brain. We employ machine-learning algorithms in combination with fluorescence imaging and adhesion studies of neuronal cells to access the biocompatibility of electrode materials. Multielectrode arrays of nanocolumnar titanium nitride comprise improved electric properties and cell-surface interaction compared to conventional electrode materials important for cell stimulation.
Erforschen der Aortenklappenstenose im mikrophysiologischen System
Authors: Dittfeld Claudia , Sonntag Frank , Tugtekin Sems-Malte 
Published: April 2024

AbstractCalcific aortic valve disease results in stenosis and patient death if aortic valve (AV) is not replaced by prosthesis. Since conventional 2D valve cell cultures and animal models that, hardly reflect the human situation, are limited, the development of an AV microphysiological tissue culture system offering the possibility to also implement human AV tissue is aim of the research. Monitoring the tissue viability, ECM remodelling and marker expression also for long time culture periods is therefore essential.
Funktionale Hydrogele in mikrofluidischen Nanoreaktoren
Authors: Obst Franziska , Appelhans Dietmar , Gaitzsch Jens 
Published: April 2024

AbstractThe developments of microfluidics and hydrogels are strongly linked to one another. Small micrometer-sized hydrogels, so-called hydrogel-dots, can be placed into the microfluidic channel to fulfil a variety of different purposes. Handling chemical reactions or governing the flow as a valve would be two of the most prominent applications of hydrogel-dots in microfluidic channels. In this article, we will cover which requirements the microfluidic device needs to meet and how hydrogels can be applied for each of the named purposes.
Copy number signatures and CCNE1 amplification reveal the involvement of replication stress in high-grade endometrial tumors oncogenesis
Authors: Marlin Regine , Loger Jean-Samuel , Joachim Clarisse , Ebring Coralie , Robert-Siegwald Guillaume , Pennont Sabrina , Rose Mickaelle , Raguette Kevin , Suez-Panama Valerie , Ulric-Gervaise Sylviane , Lusbec Sylvie , Bera Odile , Vallard Alexis , Aline-Fardin Aude , Colomba Emeline , Jean-Laurent Mehdi 
Published: April 2024

Abstract
Purpose
Managing high-grade endometrial cancer in Martinique poses significant challenges. The diversity of copy number alterations in high-grade endometrial tumors, often associated with a TP53 mutation, is a key factor complicating treatment. Due to the high incidence of high-grade tumors with poor prognosis, our study aimed to characterize the molecular signature of these tumors within a cohort of 25 high-grade endometrial cases.

Methods
We conducted a comprehensive pangenomic analysis to categorize the copy number alterations involved in these tumors. Whole-Exome Sequencing (WES) and Homologous Recombination (HR) analysis were performed. The alterations obtained from the WES were classified into various signatures using the Copy Number Signatures tool available in COSMIC.

Results
We identified several signatures that correlated with tumor stage and disctinct prognoses. These signatures all seem to be linked to replication stress, with CCNE1 amplification identified as the primary driver of oncogenesis in over 70% of tumors analyzed.

Conclusion
The identification of CCNE1 amplification, which is currently being explored as a therapeutic target in clinical trials, suggests new treatment strategies for high-grade endometrial cancer. This finding holds particular significance for Martinique, where access to care is challenging.
Self-adaptive Technique with Double Inertial Steps for Inclusion Problem on Hadamard Manifolds
Authors: Abass Hammed Anuoluwapo , Oyewole Olawale Kazeem , Aremu Kazeem Olalekan , Jolaoso Lateef Olakunle 
Published: April 2024

AbstractIn this article, we investigate monotone and Lipschitz continuous variational inclusion problem in the settings of Hadamard manifolds. We propose a forward–backward method with a self-adaptive technique for solving variational inclusion problem. To increase the rate of convergence of our proposed method, we incorporate our iterative method with double inertial steps and establish a convergence result of our iterative method under some mild conditions. Finally, in order to illustrate the computational effectiveness of our method, some numerical examples are also discussed. The result present in this article is new in this space and extends many related results in the literature.