bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between reduced b cell susceptibility to igm antibodies and reduced igd‐bearing b cells in patients with systemic lupus erythematosus
Authors: Suzuki Hiroshi , Sakurami Takehiko , Imura Hiroo 
Published: February 2007

AbstractPeripheral blood lymphocytes from 14 patients with systemic lupus erythematosus, 5 patients with rheumatoid arthritis, and 10 normal subjects were cultured for 7 days with or without anti‐IgM or anti‐IgD antibodies, and IgG‐ and IgM‐secreting cells were assayed by reverse hemolytic plaque assay. Surface immunoglobulin (Ig) isotypes on peripheral blood B cells were also examined by a direct anti‐Ig resetting reaction. In normal subjects and rheumatoid arthritis patients, the spontaneous development of IgG‐ and IgM‐secreting cells was markedly suppressed by anti‐IgM or anti‐IgD antibodies. Over 50% of peripheral blood B cells were IgD‐ and/or IgM‐bearing cells in normal subjects and in most patients with rheumatoid arthritis. In lupus patients, however, the suppression of IgG and IgM production by anti‐IgM or anti‐IgD antibodies was remarkably reduced, especially in the active stage. Furthermore, the percentage of IgD‐bearing cells in peripheral blood B cells was remarkably reduced, especially in patients with active disease. There was a good correlation between reduced susceptibility of B cells to anti‐IgM antibody‐mediated suppression and reduced percentage of IgD‐bearing cells in lupus patients.
Effect of metal chelators and antiinflammatory drugs on the degradation of hyaluronic acid
Authors: Betts W. Henry , Cleland Leslie G. 
Published: February 2007

AbstractDegradation of hyaluronic acid (measured viscometrically) by oxygen‐derived free radicals (ODFR) generated 1) by autoxidation of ferrous EDTA chelates and 2) enzymatically by xanthine oxidase and hypoxanthine (XO/HX) was studied. Degradation of hyaluronic acid by XO/HX was strongly inhibited by superoxide dismutase and catalase, whereas degradation of hyaluronic acid by autoxidation of ferrous ions was weakly inhibited by catalase and unaffected by superoxide dismutase. Both ODFR‐producing systems were inhibited by hydroxyl radical scavengers, suggesting that hydroxyl radical was the proximate damaging species in both systems. Penicillamine at concentrations of 1–5 mM stimulated hyaluronic acid degradation by ferrous EDTA chelates but inhibited degradation by the XO/HX system. Higher concentrations of penicillamine and all concentrations studied (1–100 mM) of other antiinflammatory drugs (chloroquine, gold sodium thiomalate, and salicylate) inhibited hyaluronic acid degradation by both the autoxidation and enzymatic ODFR‐producing systems, with inhibitory potency similar to that seen with known hydroxyl radical scavengers. Both systems serve as in vitro models of ODFR‐mediated tissue damage which may occur in vivo at sites of inflammation.
Quantitation and detection of isotypes of ANTI‐SS‐B antibodies by elisa and farr assays using affinity purified antigens
Authors: Venables PATRICK J. W. , Charles Peter J. , Buchanan RUSSELL R. C. , Yi Tung , Mumford Patricia A. , Schrieber Leslie , Room GERALDINE R. W. , Maini Ravinder N. 
Published: February 2007

AbstractAffinity purified SS‐B was characterized as a protein with immunoreactive polypeptides of 40K and 29K. A modified Farr assay and an enzyme linked immunosorbent assay (ELISA) were 100‐ to 1,000‐fold more sensitive than immunodiffusion and showed an association with the systemic manifestations of primary sicca syndrome and Sjögren's syndrome with systemic lupus erythematosus. The ELISA was sufficiently sensitive to detect class specific antibodies in saliva and lymphocyte culture supernatants.
The effect of native and single stranded DNA on the platelet release reaction
Authors: Dorsch Carole A. , Killmayer Jody 
Published: February 2007

AbstractNative (n) but not single stranded (ss) DNA was found to induce release of 3H‐serotonin (5‐HT) from platelets of the majority of normal individuals. However, ssDNA markedly enhanced 5‐HT release induced by heat‐aggregated IgG (aggIgG), while less enhancement was seen using nDNA. Similar enhancement was produced by polyinosinic acid but not by polyinosinic:polycytidylic acid. The ability of ssDNA to potentiate aggIgG‐induced 5‐HT release seemed specific for aggIgG, since no effect on ADP or epinephrine‐induced release was observed and thrombin‐induced release was inhibited. In contrast, nDNA in high concentrations (100 μg/ml) potentiated ADP, epinephrine, and thrombin‐induced 5‐HT release. These results suggest that ss‐and nDNA may interact with platelets by different mechanisms and provide a means by which DNA, released at sites of tissue injury, could modulate the role of platelets in the inflammatory response. The ability of DNA to enhance the aggIgG‐induced platelet release reaction may be important in immune complex diseases such as systemic lupus erythematosus.
An Evaluation of the Annual Meeting of The American Rheumatism Association
Authors: Meenan Robert F. , Mason John H. , Winfield John B. 
Published: February 2007

AbstractMembers of the American Rheumatism Association (ARA) were surveyed to determine their views on the content and quality of their annual meeting. Responses were received from 1,208 members. ARA members were generally positive about the major components of the annual meeting program, but there were significant differences in opinion between practitioners and academicians. Practitioners favored a reduction in basic science material and an increase in didactic clinical sessions. Academicians were more likely to feel that recent annual meetings have adequately addressed their educational needs. These findings have implications for the planning of future annual meetings of the ARA.
IgG and IgM Rheumatoid Factors in Rheumatoid Arthritis
Authors: Wernick Richard , Merryman Parvin , Jaffe Israeli , Ziff Morris 
Published: February 2007

AbstractPenicillamine treatment of patients with rheumatoid arthritis (RA) leads to falling titers of agglutinating IgM rheumatoid factor (RF), but its effect on IgG RF has not been described. Using specific solid phase radio‐immunoassays, we have determined serial levels of IgM RF and IgG RF in 18 patients receiving penicillamine for 1 year, and correlated the results with the change in RA activity. Mean IgM RF levels fell to 76 ± 10% (mean ± SEM) after 3 months, and 30 ± 5% of the pretreatment value after 1 year of penicillamine treatment. This decline was greater than that for total IgM (P < 0.0001), indicating a selective reduction of RF. Patients receiving maintenance doses of 750 mg/day manifested more rapid and greater decreases than did those given 250 mg/day. In contrast, serial mean IgG RF levels did not change significantly, and actually increased in 6 of 18 cases. At onset, there was a significant correlation with erythrocyte sedimentation rate for both IgM RF (r = 0.535, P = 0.05) and IgG RF levels (r = 0.570, P = 0.02). But changes in RF concentration demonstrated no correlation with changes in either erythrocyte sedimentation rate or joint score over the 1‐year period, suggesting that circulating IgM RF or IgG RF levels may be unrelated to the degree of RA activity.
Decreased Complement Mediated Binding of Antibody/3H‐dsDNA Immune Complexes to the Red Blood Cells of Patients with Systemic Lupus Erythematosus, Rheumatoid Arthritis, and Hematologic Malignancies
Authors: Taylor Ronald P. , Horgan Carol , Buschbacher Ralph , Brunner Carolyn M. , Hess Charles E. , O'Brien William M. , Wanebo Harold J. 
Published: February 2007

AbstractThe complement mediated binding of prepared antibody/3H‐dsDNA immune complexes to the red blood cells obtained from a number of patient populations has been investigated. Patients with solid tumors have binding activity similar to that seen in a normal group of individuals. However, a significant fraction of patients with systemic lupus erythematosus, rheumatoid arthritis, and hematologic malignancies have lowered binding activity compared with normal subjects. Quantitative studies indicate the lowered activity probably arises due to a decrease in complement receptors on the respective red blood cells. The potential importance and implications of these findings are briefly discussed.
Neurologic complications induced by gold treatment
Authors: Schlumpf U. , Meyer M. , Ulrich J. , Friede R. L. 
Published: February 2007

AbstractTwo patients developed a Guillain—Barre‐like syndrome and a third suffered neuropathy and myokymia during therapy with sodium aurothiomalate. The cumulative dose at the time of development of neuropathy was different in all 3 cases. Evidence for gold toxicity was suggested by clinical, electromyographic, and histologic findings, as well as the disappearance of symptoms and signs on cessation of gold injections.
Suppressor cell function and t lymphocyte subpopulations in peripheral blood of patients with progressive systemic sclerosis
Authors: Whiteside Theresa L. , Kumagai Yasuo , Roumm Alan D. , Almendinger Robin , Rodnan Gerald P. 
Published: February 2007

AbstractThree different in vitro assays—immunofluorescence with monoclonal anti‐T cell reagents, enumeration of Tγ cells, and nonspecific suppressor cell function—were used for the analysis of suppressor lymphocytes in the circulation of 28 patients with progressive systemic sclerosis (PSS, scleroderma) and 20 normal individuals. Both OKT8+ and Tγ lymphocytes were significantly reduced (P < 0.001 and P < 0.02, respectively) in patients with PSS compared with controls. The OKT4/OKT8 ratio was increased (P < 0.02). However, the mean suppressor cell index (SCI) of 1.9 (range 0.4‐6.6) for patients with PSS was not significantly different (P > 0.05) from the SCI of 2.9 (range 1.2‐14) for controls. Eleven of the patients had depressed suppressor cell function as indicated by the index value of < 1.2. In only 5 of these patients, simultaneously measured Tγ and OKT8+ cells were reduced and OKT4+ lymphocytes were concomitantly increased. There were no significant correlations between the numbers of Tγ or OKT8+ cells and the SCI in patients and controls. Neither depressed suppressor cell function nor the OKT4+/OKT8+ ratio > 4.2 (>2 SD of normal) in the patients could be related to other immunologic findings, to disease duration and severity, or to involvement of internal organs. These results suggest that depressed suppressor cell activity and immunoregulatory T cell imbalance in PSS may not be directly related to the pathogenesis of the disease.
Collagenolytic activity and collagen matrix breakdown of the articular cartilage in the pond‐nuki dog model of osteoarthritis
Authors: Pelletier Jean‐Pierre , Martel‐Pelletier Johanne , Altman Roy D. , Ghandur‐Mnaymneh Latifa , Howell David S. , Frederick Woessner J. 
Published: February 2007

AbstractRecent reports on the Pond‐Nuki model of osteo‐arthritis in the dog have provided evidence for partial disruption of the collagen network. The possibility of collagenase involvement in these localized changes was studied. Animals were killed 2, 4, 8, and 12 weeks after surgery. The left knee served as a sham‐operated control. Cartilages from femoral condyles were processed for light and electron microscopy and assayed for collagenolytic activity by a direct tissue assay, based on the measurement of digestion of endogenous cartilage collagen. Animals killed at 2 and 4 weeks showed fibrillation and mild erosion of femoral condyles, which usually progressed to ulceration by 8 and 12 weeks. Electron microscopy demonstrated fiber disruption of the midzone perilacunar collagen as early as 2 weeks after the operation. Total collagenolytic activity, measured after activation by aminophenylmercuric acetate, was significantly higher in diseased cartilage than in controls of 2, 4, and 8 weeks; the peak value was at 4 weeks. Collagenase was shown, by its specific action on type I collagen, to be present at 2 and 4 weeks; however, other metallo‐proteases may also contribute to the digestion. The correlation between increased collagenolytic activity and the early osteoarthritic changes in cartilage suggests a role of this enzyme activity in the disease process.
A new model of osteoarthritis in rabbits
Authors: Colombo C. , Butler M. , O'Byrne E. , Hickman L. , Swartzendruber D. , Selwyn M. , Steinetz B. 
Published: February 2007

AbstractA partial lateral meniscectomy procedure has been developed for the induction of a predictable and reproducible degenerative joint disease in knees of rabbits. The procedure adopted involves section of the fibular collateral and sesamoid ligaments and removal of 4‐5 mm of the anterior lateral meniscus. In most experiments the animals are killed and tissues obtained for histologic examination at 6 weeks. Section of the ligaments alone (with or without penetration of the joint space) did not result in significant pathologic change. Significant degeneration was observed in tibial and femoral cartilage when the meniscus as well as the ligaments were cut, but the most extensive lesions were seen when a piece of the anterolateral meniscus was actually removed. These lesions included fibrillation, ulceration and erosion, “clone” and osteophyte formation, loss of chondrocytes, and loss of safraninophilic staining in the articular cartilage. The incidence and distribution of lesions with time following surgery were also investigated. Lesions were observed as early as 1‐2 weeks post‐surgery and increased in number and severity up to 12 weeks. A global scoring system has been devised to permit statistical comparisons of lesion incidence and severity in different groups of rabbits. This scoring system has enabled us to test drug efficacy in the rabbit lateral meniscectomy model of osteoarthritis.
Sustained improvement of intractable rheumatoid arthritis after total lymphoid irradiation
Authors: Field Elizabeth H. , Strober Samuel , Hoppe Richard T. , Calin Andrei , Engleman Edgar G. , Kotzin Brian L. , Tanay Amir S. , Jane Calin H. , Terrell Candace P. , Kaplan Henry S. 
Published: February 2007

AbstractTotal lymphoid irradiation (TLI) was administered to 11 patients who had intractable rheumatoid arthritis that was unresponsive to conventional medical therapy, including aspirin, multiple nonsteroidal antiin‐flammatory drugs, gold salts, and D‐penicillamine. Total lymphoid irradiation was given as an alternative to cytotoxic drugs such as azathioprine and cyclophosphamide. After radiotherapy, 9 of the 11 patients showed a marked improvement in clinical disease activity as measured by morning stiffness, joint tenderness, joint swelling, and overall functional abilities. The mean improvement of disease activity in all patients ranged from 40–70 percent and has persisted throughout a 13–28 month followup period. This improvement permitted the mean daily steroid dose to be reduced by 54%. Complications included severe fatigue and other constitutional symptoms during radiotherapy, development of Felty's syndrome in 1 patient, and an exacerbation of rheumatoid lung disease in another. After therapy, all patients exhibited a profound T lymphocytopenia, and a reversal in their T suppressor/cytotoxic cell to helper cell ratio. The proliferative responses of peripheral blood mononu‐clear cells to phytohemagglutinin, concanavalin A, and allogeneic leukocytes (mixed leukocyte reaction) were markedly reduced, as was in vitro immunoglobulin synthesis after stimulation with pokeweed mitogen. Alterations in T cell numbers and function persisted during the entire followup period, except that the mixed leukocyte reaction showed a tendency to return to normal values.
Heterogeneity of bone marrow‐directed immune mechanisms in the pathogenesis of neutropenia of felty's syndrome
Authors: Abdou Nabih I. 
Published: February 2007

AbstractTwenty‐seven patients with Felty's syndrome were studied by the colony forming unit in culture (CFU‐C) assay for possible immune mechanisms within the bone marrow compartment that could contribute to the neutropenia. Depletion of bone marow suppressor T cells resulted in normal CFU‐C numbers in 7 patients. In 5 patients serum antiprecursor cell activity was detected. Blood monocytes failed to generate colony stimulating factor in 5 patients. Bone marrow‐directed immune mechanisms are heterogeneous and could play a role in the pathogenesis of neutropenia in some patients with Felty's syndrome.
67Gallium lung scans in progressive systemic sclerosis
Authors: Baron Murray , Feiglin David , Hyland Robert , Urowitz Murray B. , Shiff Bonnie 
Published: February 2007

Abstract67Gallium lung scans were performed in 19 patients with progressive systemic sclerosis (scleroderma). Results were expressed quantitatively as the 67Gallium Uptake Index. The mean total pulmonary 67Gallium Uptake Index in patients was significantly higher than that in controls (41 versus 25), and 4 patients (21%) fell outside the normal range. There were no clinical or laboratory variables that correlated with the 67gallium uptake. Increased pulmonary 67gallium uptake in scleroderma may prove useful as an index of pulmonary disease activity.
The epidemiology of giant cell arteritis. Report of a Ten‐Year Study in Shelby County, Tennessee
Authors: Smith Carolyn A. , Fidler William J. , Pinals Robert S. 
Published: February 2007

AbstractWe have studied the epidemiology of giant cell arteritis (GCA) hi an urban population in south central United States. The incidence of GCA in Shelby County, Tennessee for the years 1971 through 1980 was determined for the total population and for age‐, sex‐, and race‐specific groups. Over this period 26 cases were identified. The average annual incidence was 0.35/100,000; This was increased to 1.58/100,000 for those over the age of 50. The incidence was 7 times greater in whites than in blacks and 7 times greater in females than in males. Clinical and laboratory features of the patients were reviewed and found to be similar to those in other populations. GCA is considerably less frequent in Shelby County, Tennessee than in other areas where similar epidemiologic studies have been performed. This difference can be accounted for only in part by racial distributions. Similar studies in southern geographic areas are needed to place our findings in perspective and point to specific factors which may have etiologic significance in GCA.
Polyarthritis in rats following the systemic injection of lactobacillus casei cell walls in aqueous suspension
Authors: Lehman Thomas J. A. , Allen Janice B. , Plotz Paul H. , Wilder Ronald L. 
Published: February 2007

AbstractPostdysenteric Reiter's syndrome and the arthritis associated with jejunal bypass surgery for obesity suggest that the gastrointestinal tract is a possible source of bacterial products capable of initiating chronic inflammatory arthritis. Lactobacillus casei, a common component of the enteric flora, has a cell wall structure similar to that of arthritogenic streptococci. In this study we have demonstrated that a single intraperitoneal injection of an aqueous suspension of the cell walls of Lactobacillus casei induces a dose‐dependent, chronic, symmetric, erosive, peripheral polyarthritis in inbred LEW/N female rats. This model suggests that bacteria in the “normal enteric flora” may be a source of inflammatory products capable of inducing chronic erosive polyarthritis.
Lack of activation of C1, despite circulating immune complexes detected by two C1q methods, in patients with rheumatoid arthritis
Authors: Erik Hack C. , Eerenberg‐Belmer Anke J. M. , Lim Uwi G. , Haverman Joost , Aalberse Rob C. 
Published: February 2007

AbstractThe activation of C1 by circulating immune complexes in patients with rheumatoid arthritis was investigated. C\documentclass{article}\pagestyle{empty}\begin{document}$ \overline {{\rm{1r}}} $\end{document}C\documentclass{article}\pagestyle{empty}\begin{document}$ \overline {{\rm{1s}}} $\end{document}
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‐In)2 complexes in EDTA‐plasma, reflecting C1 activation in vivo, were slightly raised in 35 of 57 patients with rheumatoid arthritis, though most patients had elevated levels of circulating immune complexes as measured with either the 125I‐C1q binding test or the C1q solid phase assay. The activation of C1 by circulating immune complexes in vitro was investigated by measuring the generation of C\documentclass{article}\pagestyle{empty}\begin{document}$ \overline {{\rm{1r}}} $\end{document}
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‐In)2 complexes during 60 minutes at 37oC in diluted recalcified EDTA‐plasma. In 16 of the 57 patients, a slightly increased C1 activation in vitro was observed. These patients tended to have high levels of circulating immune complexes. However, the majority of the patients with high levels of circulating immune complexes showed a normal C1 activation in vitro. Therefore, it was concluded that measurement of circulating immune complexes by either of the two C1q methods in patients with rheumatoid arthritis does not imply that these circulating immune complexes are able to activate C1.
In vitro t lymphocyte proliferative response to yersinia enterocolitica in reiter's syndrome
Authors: Brenner Michael B. , Kobayashi Shigeto , Wiesenhutter Craig W. , Huberman Arthur K. , Bales Peter , Yu David T. Y. 
Published: February 2007

AbstractWe assessed the in vitro T lymphocyte tritiated thymidine (3HTdr) incorporation response of Reiter's patients in the United States to a serotype 3 strain of Yersinia enterocolitica. The mean 3HTdr incorporation response to the formalin killed form of this strain was 27,409 ± 5,488 counts per minute for 14 HLA‐B27 positive Reiter's patients compared with 5,414 ± 3,490 cpm for a control group of 11 HLA‐B27 positive normal individuals (P < 0.0005). This high response in Reiter's patients was observed with the formalin killed form of Y enterocolitica serotype 3, but not with a heat killed form or a rough mutant form derived from the same bacterial strain. Further, Y enterocolitica of a different serotype (serotype 8) which is not associated with reactive arthritis failed to induce the high proliferative response observed with the serotype 3 strain. This response indicates that T lymphocytes from spontaneous Reiter's patients are capable of recognizing and proliferating to determinant(s) on the formalin killed form of Y enterocolitica serotype 3. Since this bacterium is associated with Reiter's syndrome in Europe but not the United States, these data are consistent with the possibility that our patients have previously encountered these or similar determinants through unrecognized infection with other microorganisms.
The effects of a partially purified fraction of an ant venom in rheumatoid arthritis
Authors: Altman Roy D. , Schultz Duane R. , Collins‐Yudiskas Barbara , Aldrich Jose , Arnold Patricia I. , Arnold Patricia I. , Brown Harvey E. 
Published: February 2007

AbstractA partially purified extract of an ant venom from the South American tree ant Pseudomyrmex sp. was tested in a double‐blind, controlled study of patients with rheumatoid arthritis. Venom treated patients demonstrated an improvement in global efficacy and a decrease in the number of tender/painful joints and swollen joints. Swollen joint index improved in 60% of venom treated patients. Other parameters did not demonstrate significant change. Reduction of joint swelling was followed by symptomatic improvement that was sometimes delayed by weeks. Reactions were limited to erythema at the injection site (all patients), local pruritis (two‐thirds of the patients), and fever with malaise (one‐third of the patients). Further study of this venom in rheumatoid arthritis appears warranted in view of its apparent favorable efficacy‐to‐toxicity ratio.