bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure and binding of selected immunodominant La/SSB epitopes on human apoptotic cells
Authors: Neufing Petra J. , Clancy Robert M. , Jackson Michael W. , Tran Hai Bac , Buyon Jill P. , Gordon Tom P. 
Published: December 2005

AbstractObjectiveOpsonization of apoptotic cells by autoantibodies bound to surface membrane–translocated La/SSB antigens may initiate tissue damage in the setting of congenital heart block. By injecting pregnant mice with human anti‐La antibodies, we previously demonstrated the formation of IgG–apoptotic cell complexes in the developing mouse fetus; however, the binding of anti‐La antibodies to human‐specific epitopes could not be addressed. Accordingly, the objective of the current study was to delineate the epitope specificity of human La antibodies that are exposed on the surface of apoptotic cells.MethodsWe used fluorescence microscopy and flow cytometry to assess the binding of human anti‐La antibodies affinity purified against immunodominant epitopes of La to human cells undergoing spontaneous apoptosis, in a murine xenograft model in vivo and in cultured human fetal cardiocytes rendered apoptotic in vitro, respectively.ResultsAnti‐La antibodies bound to immunodominant epitopes of La within the NH2‐terminus and the RNA recognition motif (RRM) region of apoptotic human cells, in both xenografts and fetal cardiocytes. In contrast, human antibodies affinity purified against the COOH‐terminal La epitope did not bind apoptotic cells in either model. This defines the topology of redistributed La during apoptosis, with surface exposure of the NH2‐terminus and RRM regions. The potential importance of anti‐La NH2‐terminal and anti‐La RRM specificity was confirmed by detection of this reactivity in mothers of children with congenital heart block.ConclusionThese findings provide insight into both the molecular modification of the La autoantigen during apoptosis and the specificity of antibodies capable of binding to surface‐exposed La. Subsequent formation of surface immune complexes may lead to tissue injury in patients with autoimmune diseases such as congenital heart block.
The PTPN22 620W allele is a risk factor for Wegener's granulomatosis
Authors: Jagiello Peter , Aries Peer , Arning Larissa , Wagenleiter Sonja E. N. , Csernok Elena , Hellmich Bernhard , Gross Wolfgang L. , Epplen Joerg T. 
Published: December 2005

AbstractObjectiveAnalyses of families with multiple autoimmune disorders have revealed a functional polymorphism, 620W, in the intracellular tyrosine phosphatase gene PTPN22 as a predisposing factor for type 1 diabetes, seropositive rheumatoid arthritis, systemic lupus erythematosus, and Hashimoto thyroiditis, and the presence of the PTPN22 protein appears to herald the development of autoantibodies in these disorders. This study therefore examined whether the functionally relevant PTPN22 polymorphism is associated with Wegener's granulomatosis (WG).MethodsA population‐based study was performed for the PTPN22 polymorphism in 199 patients with WG and in 399 healthy individuals. The R620W variation was investigated by simple restriction fragment‐length polymorphism analysis.ResultsThe PTPN22 620W allele frequency was significantly increased in antineutrophil cytoplasmic antibody (ANCA)–positive WG patients compared with healthy controls (P < 0.001). The association was particularly striking in patients with kidney, lung, eye, and peripheral nervous system involvement (i.e., those with generalized WG).ConclusionThe PTPN22 620W allele appears to be involved in the pathogenesis of WG, and ANCA positivity seems to be the hallmark.
Acute CD4+ T lymphocyte–dependent interleukin‐1–driven arthritis selectively requires interleukin‐2 and interleukin‐4, joint macrophages, granulocyte–macrophage colony‐stimulating factor, interleukin‐6, and leukemia inhibitory factor
Authors: Lawlor Kate E. , Wong Peter K. K. , Campbell Ian K. , Rooijen Nico van , Wicks Ian P. 
Published: December 2005

AbstractObjectiveTo further investigate the effects of interleukin‐1 (IL‐1) in immune‐mediated joint inflammation, we examined the role of IL‐2, Th1 interferon‐γ (IFNγ), and Th2 (IL‐4) cytokines, joint macrophages, and macrophage‐derived cytokines (IL‐12 p40, IL‐6, leukemia inhibitory factor [LIF], oncostatin M [OSM], and granulocyte–macrophage colony‐stimulating factor [GM‐CSF]) in a CD4+ T lymphocyte–dependent model of acute arthritis.MethodsMethylated bovine serum albumin (mBSA)/IL‐1–induced arthritis was elicited in wild‐type, gene‐knockout, and monoclonal antibody–treated mice. Synovial lining macrophages were selectively depleted by intraarticular injection of clodronate liposomes prior to disease induction. The severity of arthritis was assessed histologically.ResultsMice deficient in IL‐2 were almost completely protected from arthritis, and neutralization of IL‐4 reduced the severity of disease. In contrast, arthritis severity and resolution appeared to be independent of IFNγ. Synovial lining macrophage depletion markedly reduced arthritis severity. IL‐6 or LIF deficiency was only modestly protective, although as previously reported, GM‐CSF deficiency conferred profound disease resistance. IL‐12 p40–deficient mice (which lack IL‐12 and IL‐23) and OSM receptor–deficient mice were susceptible to mBSA/IL‐1–induced arthritis.ConclusionAcute mBSA/IL‐1–induced arthritis is dependent on IL‐2 and IL‐4, but not IFNγ. In vivo, the Th1/Th2 paradigm may be distorted by the presence of macrophage‐derived cytokines such as IL‐1. Synovial lining macrophages are essential in mBSA/IL‐1–induced arthritis. However, the requirement for macrophage‐derived cytokines is selective; that is, IL‐6, LIF, and especially GM‐CSF are necessary, but IL‐12, IL‐23, and OSM are dispensable. IL‐1 may therefore influence both adaptive and innate immune mechanisms in acute inflammatory arthritis.
Design characteristics that affect speed of information access and clarity of presentation in an electronic neuroanatomy atlas
Authors: Stewart P.A. , Nathan N. , Nyhof‐Young J. 
Published: November 2005

AbstractFunctional Neuroanatomy, an interactive electronic neuroanatomical atlas, was designed for first year medical students. Medical students have much to learn in a limited time; therefore a major goal in the atlas design was that it facilitate rapid, accurate information retrieval. To assess this feature, we designed a testing scenario in which students who had never taken a neuroanatomy course were asked to complete two equivalent tests, one using the electronic atlas and one using a comparable hard copy atlas, in a limited period of time. The tests were too long to be completed in the time allotted, so test scores were measures of how quickly correct information could be retrieved from each source. Statistical analysis of the data showed that the tests were of equal difficulty and that accurate information retrieval was significantly faster using the electronic atlas when compared with the hard copy atlas (P < 0.0001). Post‐test focus groups (n = 4) allowed us to infer that the following design features contributed to rapid information access: the number of structures in the database was limited to those that are relevant to a practicing physician; all of the program modules were presented in both text and image form on the index screen, which doubled as a site map; pages were layered electronically such that information was hidden until requested, structures available on each page were listed alphabetically and could be accessed by clicking on their name; and an illustrated glossary was provided and equipped with a search engine. Clin. Anat. 20:93–110, 2007. © 2005 Wiley‐Liss, Inc.
Organotin Perfluorooctanesulfonates as Air‐Stable Lewis Acid Catalysts: Synthesis, Characterization, and Catalysis
Authors: An De Lie , Peng Zhihong , Orita Akihiro , Kurita Akinobu , Man‐e Sumiyo , Ohkubo Kei , Li Xingshu , Fukuzumi Shunichi , Otera Junzo 
Published: December 2005

AbstractThe reactions of 1,3‐dichloro‐1,1,3,3‐tetrabutyldistannoxane and dialkyltin dihalides with silver perfluorooctanesulfonate provided the corresponding sulfonates as hydrates. The number of water molecules (n) of hydration was dependent on the conditions. The distannoxane derivative was identified as n from 0.5 to 6, while in the hydrated mononuclear species and DMSO complexes n varied widely from 4 to 13. 119Sn NMR spectroscopy and conductivity measurements indicated the ionic dissociation of these compounds in solution. These compounds exhibited unusually high solubility in polar organic solvents. The ionic dissociation together with facile hydration probably causes the unusual solubility. The Lewis acidity of these compounds was found to be high among organotin derivatives on the basis of ESR spectra of superoxide/metal‐ion complexes. In contrast to well‐known organotin triflates, these compounds suffered no hydrolysis upon storage in open air. The high catalytic activity of the distannoxane 1 was exemplified for various carbon–carbon bond‐forming reactions, such as Mukaiyama–aldol as well as ‐Michael reactions and allylation of aldehydes.
Smoking influences aberrant CpG hypermethylation of multiple genes in human prostate carcinoma
Authors: Enokida Hideki , Shiina Hiroaki , Urakami Shinji , Terashima Masaharu , Ogishima Tatsuya , Li Long‐Cheng , Kawahara Motoshi , Nakagawa Masayuki , Kane Christopher J. , Carroll Peter R. , Igawa Mikio , Dahiya Rajvir 
Published: December 2005

AbstractBACKGROUNDAberrant CpG methylation profiles of gene promoters and their correlation with advanced pathologic features have been well investigated in prostate carcinoma (PC). Several case–control and prospective studies have revealed a positive association between current smoking and PC. The authors hypothesized that smoking influences both progression and prognosis of PC through CpG hypermethylation of related genes.METHODSA total of 164 PC patients (52 current, 30 former, and 82 never smokers) and 69 benign prostatic hyperplasia (BPH) patients were examined by methylation‐specific PCR (MSP) for 3 genes: adenomatous polyposis coli (APC), glutathione S‐transferase pi (GSTP1), and multidrug resistance one (MDR1). The methylation status of representative samples was confirmed by bisulfite DNA sequencing analysis. The newly defined methylation score (M‐score) of each sample is the sum of the corresponding log hazard ratio (HR) coefficients derived from multivariate logistic regression analysis for pathology (BPH vs. PC), and was related to clinical and pathologic outcome including smoking status.RESULTSThe M‐score was significantly higher in the current smokers than in never smokers (P = 0.008). Spearman rank correlation test demonstrated a significant correlation between pack‐years smoked and M‐score in PCs (P = 0.039). Significant correlation of the M‐score methylation was observed with high pT category (P < 0.001), high Gleason sum (P < 0.001), high preoperative prostate‐specific antigen (PSA) (P = 0.041), and advanced pathologic features. In addition, Gleason sum was significantly associated with PSA failure‐free probability as a poor outcome (P = 0.020).CONCLUSIONSThis is the first study to demonstrate significant correlation of the methylation status of multigenes with smoking status in PC. Smoking status may influence both progression and prognosis of PC through CpG hypermethylation of related genes. Cancer 2006. © 2005 American Cancer Society.
The efficacy of voice therapy in patients after treatment for early glottic carcinoma
Authors: van Gogh Christine D. L. , Verdonck‐de Leeuw Irma M. , Boon‐Kamma Brigitte A. , Rinkel Rico N. P. M. , de Bruin M. Diana , Langendijk Johannes A. , Kuik Dirk J. , Mahieu Hans F. 
Published: December 2005

AbstractBACKGROUNDAfter treatment for early glottic carcinoma, a considerable number of patients end up with voice problems that interfere with daily life activities. The objective of this randomized and controlled study was to assess the efficacy of voice therapy in these patients.METHODSOf 177 patients, 6–120 months after treatment for early glottic carcinoma, 70 patients (40%) suffered from voice impairment based on a 5‐item screening questionnaire. Approximately 60% of those 70 patients were not interested in participating in the current study. Twenty‐three patients who were willing to participate were assigned randomly either to a voice therapy group (n = 12 patients) or to a control group (n = 11 patients). Multidimensional voice analyses (the self‐reported Voice Handicap Index [VHI], acoustic and perceptual voice quality analysis, videolaryngostroboscopy, and the Voice Range Profile) were conducted twice: before and after voice therapy or with 3 months in between for the control group.RESULTSStatistical analyses of the difference in scores (postmeasurement minus premeasurement) showed significant voice improvement after voice therapy on the total VHI score, percent jitter, and noise‐to‐harmonics ratio in the voice signal and on the perceptual rating of vocal fry.CONCLUSIONSVoice therapy proved to be effective in patients who had voice problems after treatment for early glottic carcinoma. Improvement not only was noticed by the patients (VHI) but also was confirmed by objective voice parameters. Cancer 2006. © 2005 American Cancer Society.
Chelate [2‐(Iminoethyl)pyridine N‐oxide]metal Complexes – Synthesis and Structural Comparison with Their Chemically Related 2‐(Iminoethyl)pyridine‐Derived Systems
Authors: Nienkemper Katrin , Kotov Vasily V. , Kehr Gerald , Erker Gerhard , Fröhlich Roland 
Published: December 2005

AbstractThe N,O‐chelate ligands 2‐(iminoethyl)pyridine N‐oxide (2a) and 2‐(iminoethyl)‐6‐isopropylpyridine N‐oxide (2b) were prepared by conventional synthetic routes, the latter involving a variant of the Reissert–Henze reaction. Treatment of 2a with FeCl2 resulted in a deoxygenation reaction of the ligand and formation of the salt [bis{2‐(iminoethyl)pyridine}FeCl]+[FeCl4]– (18a). In contrast, the reaction of 2a with PdCl2 or CoCl2 cleanly furnished the six‐membered chelate [κN,O‐2(iminoethyl)pyridine N‐oxide]MCl2 complexes (19a, M = Pd) or (20a, M = Co), respectively, which were both characterised by X‐ray diffraction. Treatment of 2b with [NiBr2(dme)], followed by crystallisation from THF, gave the complex [(κN,O‐2b)NiBr2(THF)] (21b), which features a distorted trigonal‐bipyramidal coordination geometry of the central metal atom. The reaction of 2a with [NiBr2(dme)] gave the structurally related complex [(κN,O‐2a)NiBr2(κO‐2a)] (21a). The N,O‐chelate Pd complex 19a was shown to be an active catalyst for the Suzuki coupling reaction. The ligand systems 2a,b and their related 2‐(iminoethyl)pyridines 3a,b and a variety of metal complexes of ligands 3 were also prepared and characterised for comparison by X‐ray diffraction. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Solid‐State Luminescence and π‐Stacking in Crystalline Uranyl Dipicolinates
Authors: Harrowfield Jack M. , Lugan Noël , Shahverdizadeh Gholam H. , Soudi Ali A. , Thuéry Pierre 
Published: December 2005

AbstractCrystal structure determinations on the hydrated forms of the complexes M2UO2(dipic)2, M = H, Rb, Cs, dipic = dipicolinate = pyridine‐2,6‐dicarboxylate, all of which show strong solid‐state luminescence from the uranyl centre, have as a common feature extended π‐stacking arrays of the dipicolinate ligands. Comparisons with related but non‐luminescent complexes indicate that these stacking arrays may be associated with efficient energy transfer from the dipicolinate “antennae” to the uranyl unit. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
The Coordination Chemistry of 1,4‐Diazepan‐6‐amine
Authors: Romba Jens , Kuppert Dirk , Morgenstern Bernd , Neis Christian , Steinhauser Stefan , Weyhermüller Thomas , Hegetschweiler Kaspar 
Published: December 2005

AbstractThe new, tridentate, facially coordinating ligand 1,4‐diazepan‐6‐amine (daza) has been prepared from ethane‐1,2‐diamine and 2,3‐dibromo‐1‐propanol in a seven‐step procedure with an overall yield of 22 %. The conformation of the free ligand has been elucidated by pH‐ and temperature‐dependent 1H NMR spectroscopy and by a single‐crystal X‐ray structure analysis of H3dazaCl3·H2O. A twisted chair with a predominantly equatorial orientation of the primary amino group has been established for daza and its protonation products Hxdazax+ (1 ≤ x ≤ 3). The formation constants of [M(daza)]2+ and [M(daza)2]2+ have been determined in aqueous solutions for M = NiII, CuII, ZnII, CdII, and CoII by potentiometric and spectrophotometric measurements, and a remarkably high stability has been found for the bis complexes ML2 in comparison to the mono complexes ML. This effect is discussed in terms of the particular steric requirements of the daza ligand. [Cu(daza)Cl2], [Ni(daza)2]Cl2·3.2H2O and [Zn(daza)2]SO4·5H2O have been characterized by single‐crystal X‐ray analyses. Aerial oxidation of Co2+ in the presence of daza results in the formation of the inert[Co(daza)2]3+, which was isolated as a mixture of the cisand trans isomers. These two isomers were separated by chromatographic methods and identified by NMR spectroscopy and single‐crystal X‐ray structure analysis of cis‐[Co(daza)2][ZnBr4]Br·H2O and trans‐[Co(daza)2]3[ZnBr4]2Br5·4H2O. The redox potentials of [Ni(daza)2]3+/2+ (1.04 V vs. NHE) and [Co(daza)2]3+/2+ (–0.21 V vs. NHE) were determined by cyclic voltammetry. The values are slightly more positive than for corresponding complexes with related cyclic triamines. This effect is again discussed in terms of the particular steric requirements of these ligands.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Evidence for Hydrogen‐Bond‐Mediated Exchange Coupling in an Aqua‐Bridged CuII Dimer: Synthesis, Magnetic Study and Correlation with Density Functional Calculations
Authors: Talukder Pritha , Sen Soma , Mitra Samiran , Dahlenberg Lutz , Desplanches Cédric , Sutter Jean‐Pascal 
Published: December 2005

AbstractA rare example of a mono‐aqua‐bridged copper(II) dimer,namely [Cu2(μ2‐H2O)L2(H2O)2](ClO4)2·2H2O (1), has been synthesised and its structure determined by single‐crystal X‐ray diffraction. Each CuII centre is in a square‐pyramidal surrounding formed by a Schiff base and a terminal H2O ligand; a second H2O ligand, which acts as a bridge, is common to two metal ions. Hydrogen bonds between the H2O ligand and the Schiff base of the adjacent centre complete the intra‐dimer linkages. This compound exhibits an antiferromagnetic behaviour (J = –13.2 cm–1, H = –JS1·S2), and density functional calculations clearly establish that the main exchange does not proceed through the bridging H2O but is mediated by the intra‐dimer H‐bonds.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Attentional modulation of emotional stimulus processing: An fMRI study using emotional expectancy
Authors: Bermpohl Felix , Pascual‐Leone Alvaro , Amedi Amir , Merabet Lotfi B. , Fregni Felipe , Gaab Nadine , Alsop David , Schlaug Gottfried , Northoff Georg 
Published: November 2005

AbstractWe used emotional expectancy to study attentional modulation in the processing of emotional stimuli. During functional magnetic resonance imaging (fMRI), volunteers saw emotional and neutral expectancy cues signaling the subsequent presentation of corresponding emotional or neutral pictorial stimuli. As a control, emotional and neutral pictures were presented without preceding expectancy cue, resulting in a 2 × 2 factorial design with the factors “expectancy” and “emotion.” Statistical analysis revealed a significant positive interaction effect between these factors in the medial prefrontal cortex (MPFC, Brodmann area [BA] 9/10), amygdala, and dorsal midbrain. In all these regions, expectancy augmented the neural response to emotional but not to neutral pictures. Time course analysis of raw data suggests that this augmented activation was not preceded by baseline increases in MPFC and amygdala during the period of emotional expectancy. In a post‐scanning session, the paradigm was presented for a second time to allow emotional intensity rating. Again, a significant interaction between expectancy and emotion was observed, with intensity ratings specifically enhanced in emotional photographs preceded by expectancy. There was a positive correlation between intensity ratings and blood oxygenation level‐dependent (BOLD) signals in the left amygdala. We conclude that specific components of the emotion network show enhanced activation in response to emotional stimuli when these are preceded by expectancy. This enhancement effect is not present in neutral pictures and might parallel accentuated subjective feeling states. Hum. Brain Mapp 2005. © 2005 Wiley‐Liss, Inc.
Persistent posttreatment depressive symptoms in patients with head and neck cancer
Authors: Karnell Lucy Hynds , Funk Gerry F. , Christensen Alan J. , Rosenthal Eben L. , Magnuson J. Scott 
Published: December 2005

AbstractBackground.This study examined the prevalence and risk factors of persistent (versus short‐term) depressive symptoms in patients with head and neck cancer.Methods.Patients with 10+ and 18+ posttreatment Beck Depression Inventory scores for 6 or more months during their first year were identified. Regression analyses determined risk factors associated with persistently high scores.Results.Of the 148 patients, 25.0% and 7.4% were persistently above the 10+ and 18+ cutoff scores, respectively (compared with 33.6% to 44.2% and 9.2% to 18.6% when measured at single points across this time period.) The strongest predictor of persistent posttreatment depressive symptoms was pretreatment depressive symptoms.Conclusions.The percentage of patients with persistently high levels of depressive symptoms, although considerable, is substantially lower when patients with transient mood disorders are omitted. A screening tool that determines high levels of pretreatment depressive symptoms could identify patients at high risk of experiencing posttreatment depression who would be good candidates for clinical intervention. © 2005 Wiley Periodicals, Inc. Head Neck 28: 453–461, 2006
A laccase study by electrospray ionization Fourier transform ion cyclotron resonance MS: copper depletion, glycoforms and stability
Authors: Marjasvaara Asse , Kruus Kristiina , Vainiotalo Pirjo 
Published: November 2005

AbstractThe molecular properties and stability of a laccase from the white‐rot fungus Trametes hirsuta (ThL) were studied to exploit the unique capability of electrospray ionization mass spectrometry (ESI‐MS) to monitor conformational and molecular‐based heterogeneities and metal ion binding simultaneously. Acid and organic solvents were applied as denaturing agents. In aqueous acidic solution, ThL existed in two major forms, distinguished by their mass difference; in addition to these, two other forms were detected. This molecular heterogeneity was due to the variable glycan content of ThL. Additionally, copper‐depleted forms of laccase were observed in mass spectra measured from aqueous acidic solution. A small amount of organic solvent (acetonitrile, CH3CN) increased the loss of one Cu atom from folded states and led to unfolding. In the unfolded state, ThL was depleted of all four copper atoms, and the charge state distribution was shifted to lower mass‐to‐charge region. Thus, denaturation took place in two stages: first, the loss of one Cu resulting in an inactive form; second, complete denaturation with the loss of the three remaining Cu atoms. After all coppers were lost, ThL was unfolded, as was clearly seen in the increased number of charge states in the mass spectra. Different stabilities of the glycoforms were observed in the denaturation triggered in acid and organic solvents. Copyright © 2005 John Wiley & Sons, Ltd.