bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytologic characteristics of clear‐cell sarcoma of the kidney (CCSK) in fine‐needle aspiration biopsy (FNAB): A report of 4 cases
Authors: Drut Ricardo , Pomar Mabel 
Published: February 2007

AbstractThe cytologic findings observed in fine‐needle aspiration biopsy (FNAB) of four cases of clear‐cell sarcoma of the kidney (CCSK) are reported. Smears contained a monomorphicpopulation of cells exhibiting non‐descriptive cytoplasm and almost nude round to oval nuclei with smooth contour, evenly dispersed fine chromatin granules, and one or two small nucleoli. Some nuclei (approximately 40%) had a bar resulting from grooving or folding of the nuclear membrane. Comparison with smears of classical Wilms' tumor and malignant rhabdoid tumor allowed to recognize a distinctive pattern, different from other tumors of the kidney in in fancy. The recognition of the CCSK cytologic pattern justifies the usage of aggressive preoperative chemotherapy protocols or the indication of surgery avoiding delays.
Pneumocystis carinii in fna of the thyroid
Authors: Walts Ann E. , Pitchon Howard E. 
Published: February 2007

AbstractWe report the diagnosis of Pneumocystis carinii (PC) in a fine‐needle aspirate (FNA) from the thyroid of a human immunodeficiency virus infected (HIV+) male receiving aerosolized pentamidine as prophylaxis for Pneumocystis carinii pneumonia (PCP). The clinical diagnosis prior to FNA was multinodular goiter. The patient did not have pulmonary symptoms nor previous diagnosis of PCP at the time of the aspirate diagnosis. Recently, extrapulmonary Pneumocystis carinii (EPC) has been reported with increasing frequency in HIV+ patients receiving prophylactic aerosolized pentumidine. Awareness of extrapulmonary presentations of Pneumocystis carinii infection is a prerequisite for accurate Cytologic diagnosis.
Role of fine‐needle aspiration biopsy in the assessment of sacrococcygeal masses
Authors: Cristallini Enrico G. , Ascani Stefano , Paganelli Cristina , Peciarolo Antonella , Bolis Giovanni B. 
Published: February 2007

AbstractWhile fine‐needle aspiration (FNA) allows the diagnosis of deep masses, it does not always permit histologic classification. In the case of sites such as the sacrococcygeal region, in which a large number of benign or malignant, epithelial or connective, primary or secondary tumors may arise, it is necessary to choose between conservative treatment and radical surgery. This means that preoperative diagnosis must be as certain as possible. In order to increase the diagnostic accuracy of the method, when even immunohistochemistry is of no aid, it is useful to examine the histologic architecture of the specimen by preparing cell blocks from the aspirates. We report the comparative findings from the examination of the histologic architecture of tissue fragments embedded in methacrylate prepared from the aspirates of four cases of tumors: two chordomas, one metastasis of renal clear‐cell carcinoma, and one of mucus‐secreting carcinoma, all of them arising in the sacrococcygeal region.
Intraepithelial neoplasia mimicking microinvasive squamous‐cell carcinoma in endocervical brushings
Authors: Covell Jamie L , Frierson Henry F. 
Published: February 2007

AbstractThe interpretation of endocervical brush specimens requires familiarity with the various benign, atypical, dysplastic, and invasive neoplastic changes in cells located in the endocervical canal There are several pitfalls in the cytologic evaluation of brush specimens that may result in diagnostic difficulty and error. We report seven cases of high‐grade squamous intraepithelial lesions that were sampled by endocervical brushing and confirmed by cone biopsy or hysterectomy in which the cytologic changes in the Papanicolaou smears mimicked those of microinvasive sauamous‐cell carcinoma. The cells in the smears were compared with those in the tissue sections to determine their sites of origin. Results of this study indicate that cells with features fulfilling the criteria for microinvasive carcinoma were found primarily in brushing smears and corresponded with the features of cells involving the endocervical glands rather than those in the surface epithelium. We conclude that the criteria that have been promulgated for the cytologic diagnosis of microinvasive squamous‐cell carcinoma have limited value in the examination of endocervical brush specimens.
Cervicovaginal cytology in carcinosarcoma [malignant mixed mullerian (mesodermal) tumor] of the uterus
Authors: Costa Michael J. , Tidd Cecelia , Willis Dean 
Published: February 2007

AbstractWe examined the cervicovaginal cytology (PAP) findings in a series of 21 endometrial and 2 cervical carcinosarcomas (CS) [malignant mixed mullerian (mesodermal) tumors] (MMMT). Initial cytology diagnosis was positive for cancer in 14 of 23 cases (sensitivity of 61%); however, CS was correctly identified only 2 times. The remaining 12 cancer diagnoses were carcinomas: 10 adenocarcinomas (4 endometrial, 1 cervical, and 5 adenocarcinomas not otherwise specified), 1 squamous carcinoma, and 1 poorly differentiated carcinoma. Seventeen smears were available for review, all 8 false negative, one unsatisfactory, and 8 of 14 true positive smears. One false negative smear showed rare clusters diagnostic of adenocarcinoma. The remaining 7 false negative smears showed 3 high grade squamous dysplasias (two with additional findings: one showed atypical spindle cells and the other showed endometrial stromal cells), 2 extensive repair changes, and 2 negatives. The unsatisfactory smear showed atypical spindle cells. Review of the 8 true positive smears confirmed malignant spindle cells in the two cases originally identified as CS by cytology and in 3 other cases originally identified as adenocarcinoma. Of 9 smears positive for cancer available for review, 4 showed only carcinoma cells. PAP positive for cancer was associated with high stage at presentation (P <.025) and recurrent disease (P <.001) (even among stage I or II patients, P <.025). PAP positive for cancer showed no association with depth of myometrial invasion, size, grade, or histologic type of carcinosarcoma. The results of this study demonstrate the importance of consultative cytology reporting in which recommendations for appropriate biopsy are included in the report. The sensitivity of PAP could be increased to 83% (19/23 of cytologic findings would have led to biopsy recommendations). With consultative reporting, we can communicate that a biopsy is needed to establish a definitive diagnosis in many cases. For this reason, the confusing and potentially clinically damaging discrepancy between cytology and histology is minimized.
Fine‐needle aspiration cytology of multicystic mesothelioma
Authors: Devaney Kenneth , Kragel Peter J. , Devaney Eric J. 
Published: February 2007

AbstractThis report details the cytologic findings from a case of peritoneal multicystic mesothelioma (MCM). Fine‐needle aspiration of a 20 cm abdominal mass in a 31‐year‐old man yielded a specimen which consisted of a monomorphous population of mesothelial cells lacking cytologic atypia which were arranged in three patterns: monolayered sheets, single cells, and two‐cell‐thick strands of mesothelial cells with little or no intervening stroma. The background was clean, without necrotic debris or abundant inflammatory cells. The mesothelial cells were not arranged in prominent papillary formations; mitotic figures were not found. The mesothelial cells were cytokeratin positive and vimentin positive, and negative for carcinoembryonic antigen (CEA) and factor VIII. The FNA findings from MCM should be distinguished from those of a variety of other abdominal lesions, including cystic lymphangioma, ovarian and primary peritoneal epithelial tumors, necrotic tumors with cystic degeneration, developmental cysts, and infectious cysts.
Fine‐needle aspiration cytology in fibromatoses
Authors: Zaharopoulos Paul , Wong Jick Y. 
Published: February 2007

AbstractFine‐needle aspiration (FNA) cytology was performed in seven cases of fibromatosis of variable types with tumorous clinical presentation. These included: four cases of musculoaponeurotic fibromatosis, two in posterior neck muscles, one in anterior neck muscles and one in intercostal muscles; one case of fibromatosis of the breast; and two cases of fibromatosis colli in neonates. In all cases the specimens contained connective tissue with many fibroblast‐like cells, lacking features which could indicate a malignant lesion.The findings in these cases indicate that, although by FNA cytology in fibromatoses a specific diagnosis for each pathologic entity may not be easily reached, in the proper clinical setting the cytologic findings can be of sufficient relevance to offset the need for an open tissue biopsy, where there are valid reasons against a surgical intervention.
Peritoneal cytology of uncommon ovarian tumors
Authors: Valente Philip T. , Daniel Schantz H. , Edmonds Pamela R. , Hanjani Parviz 
Published: February 2007

AbstractPeritoneal cytology has been well established as a diagnostic and staging tool in the management of the common epithelial tumors of ovary. Germ cell, mesenchymal, and sex‐cord stromal tumors are much less frequently encountered in peritoneal specimens, often with cytologic features that may pose problems in differential diagnosis. This report presents the cytomorphology of the ascitic fluid in cases of endodermal sinus tumor, dysgerminoma, and Sertoli‐Leydig‐cell tumor, and peritoneal washings in a case of ovarian malignant mixed mullerian tumor. The cytologic features of Sertoli‐Leydig‐cell tumors have not been well described. Careful correlation of peritoneal cytologic findings, cell‐block preparations, and immunocytochemistry with the cytohistologic features of these tumors is crucial for correct tumor classification.
Immediate interpretation of FNA smears from the head and neck region
Authors: Sauer Torill , Freng Atle , Djupesland Per 
Published: February 2007

AbstractFNA smears from 540 patients, investigated for visible and/or palpable lesions in the head and neck, examined immediately during consultation have been compared with the final cytologic diagnoses and, when possible, with histologic results. Preliminary and final cytologic diagnoses differed in 25 cases (4.6%). Major discrepancies as to whether a lesion was benign or malignant occurred in 15 cases (2.8%). Histologic follow‐up was available for 188 lesions (35%). There were 5 false‐positive (2.6%) and 9 false‐negative (4.7%) diagnoses, giving a sensitivity of 90.6% and a specificity of 94.6%. The main diagnostic problem was benign, reactive lymphadenitis versus malignant lymphoma, which was responsible for 11 of 14 erroneous cytologic diagnoses (3 false‐positive and 8 false‐negative smears). © 1992 Wiley‐Liss, Inc.
Two AgNOR counts in fine‐needle aspirates of lymphoproliferative disorders compared with acridine orange flow cytometry
Authors: Mourad Walid A. , Katz Ruth L. , Sembera Doris , Neely Atkinson E. , El‐Naggar Adel K. 
Published: February 2007

AbstractStudies have shown that argyrophilic nucleolar organizer region‐associated proteins (AgNORs) may correlate with DNA ploidy and/or proliferative activity in neoplastic and non‐neoplastic conditions. However, studies have estimated only the mean AgNOR counts. Here we used two AgNOR counts, one of which may correlate with DNA ploidy and the other with proliferative activity. The mean AgNOR count (mAgNOR) was defined as the mean number of AgNORs/nucleus in 100 cells and may represent DNA or RNA index. The percentage of nuclei exhibiting 5 or more AgNORs/nucleus (pAgNOR) may reflect proliferative activity. These two AgNOR counts were correlated with results from acridine orange flow cyotmetry in 50 fine‐needle aspirate (FNA) smears of nodal and extranodal sites, including three cases of reactive lymphadenopathy and 47 cases of non‐Hodgkin's lymphoma. The mean mAgNOR count in the diploid specimens was 2.03 (±0.74 SD) and 2.62 (±0.73 SD) in the aneuploid tumors (P < 0.0001). Samples with a low RNA index had mean mAgNOR of 1.80 (±0.41 SD), whereas those with high RNA had a mean mAgNOR of 2.93 (±0.86 SD) (P <0.0001). Lesions with low proliferative index, determined by flow cytometry, had a mean pAgNOR of 4%, whereas those with intermediate and high proliferative indices had a mean pAgNOR of 16% (P <0.0001). A similar but less significant correlation existed between RI and pAgNOR (P < 0.005). We conclude that the two AgNOR counting methods may reliably reflect cell kinetics and distinguish ploidy from proliferative activity, making them useful adjuncts to flow cytometry in limited cytology specimens and small biopsy samples. © 1992 Wiley‐Liss. Inc.
The utility of ancillary studies in pediatric FNA cytology
Authors: Marion Gurley A. , Silverman Jan F. , Lassaletta Margarita M. , Wiley John E. , Tate Holbrook C. , Joshi Vijay V. 
Published: February 2007

AbstractWe evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work‐up of pediatric fine‐needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5‐year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, consisting of immunoperoxidase staining of direct smears and/or cell blocks or flow cytometric immunophenotyping, were performed. The studies were adequate in 14 cases (60.9%), suboptimal in five cases (21.7%), and inadequate in four cases (17.4%). Of the adequate and suboptimal cases, the ICC data helped to narrow the differential diagnosis or classify the disease process in eight cases (42.1 %), confirmed cytologic impression in nine cases (47.4%), and gave contradictory results in two cases (10.5%). Adequate material for electron microscopy (EM) was obtained in 14/19 cases (73.7%). Ultrastructural studies were diagnostic, or helped classify the disease process in five cases (35.7%), confirmed the cytologic impression in four cases (28.6%), helped exclude diagnostic considerations in three cases (21.4%), and were judged to be non‐contributory in two cases (14.3%). Cytogenetic studies revealed six of seven cases (all neoplasms) to have abnormal karyotypes. Special stains for organisms performed on smears from 25 cases including Ziehl‐Neelsen, Gomori methenamine silver (GMS), Gram, and Warthin‐Starry (WS) were negative except for 1/10 GMS and 4/9 Gram stains. In summary, we found that with appropriate case selection, ancillary studies performed on aspirated material can provide useful information in pediatric FNA cytology. © 1992 wiley‐Liss, Inc.
Selected monoclonal antibodies can increase the accuracy of cytodiagnosis of neoplastic effusions of cryptic origin expanded in a short term culture
Authors: Mottolese M. , Cianciulli A. , Venturo I. , Perrone Donnorso R. , Salzano M. , Benevolo M. , Bigotti G. , Natali P. G. 
Published: February 2007

AbstractThe use of a selected panel of monoclonal antibodies (MoAb) to tumor associated antigens (TAA) in immunocytochemical (IIC) tests has been shown, in a preliminary study, to be a powerful diagnostic tool for the identification of the primary solid tumor causing metastatic effusion. Despite this improvement in a minority of neoplastic fluids a number of different causes may still determine false negative (FN) immunocytochemical diagnoses. The aim of the present study was to confirm the diagnostic accuracy of this panel of MoAb. This was done by analyzing in IIC tests a larger number of effusions and by evaluating whether the expansion in short term culture of those fluids with an uncertain malignant morphology could provide an adequate cellular substrate for immunocytodiagnosis. The analysis of 314 effusions confirmed the results of the pilot study and demonstrated that the combination of short term culture and immunocytochemical assays can further increase the sensitivity of this novel diagnostic procedure from 84.3% to 95.3%. © 1992 Wiley‐Liss. Inc.
Fine‐needle aspiration biopsy of hepatic papillary cystadenocarcinoma in Caroli's disease
Authors: Tarroch Xavier , Tallada Natalia , Castells Carme , Garcia Magdalena 
Published: February 2007

AbstractA case of papillary adenocarcinoma arising in Caroli's disease (CD) in a 25‐yr‐old woman is reported. The diagnosis of malignancy was made by ultrasound‐guided, fine‐needle aspiration biopsy (FNAB). As there were no metastasis, a liver transplant was performed and the diagnosis was histologically confirmed. Only 22 cases of malignant transformation of CD have been reported to date, all among middle‐aged subjects (mean age: 52 yr) of both sexes. Most of these reported cases (83%) were found to be bile duct adenocarcinomas with occasional reports of hepatocellular and undifferentiated carcinomas. Our case is the youngest reported to date and the first to be diagnosed cytologically. © 1992 Wiley‐Liss, Inc.
Whipple's disease involving the mesenteric lymph nodes diagnosed by fine‐needle aspiration
Authors: Saleh Husain , Williams Thomas M. , Minda Justina M. , Gupta Prabodh K. 
Published: February 2007

AbstractAlthough it traditionally presents with signs and symptoms of small intestine involvement, such as diarrhea and malabsorption, Whipple's disease (WD) can involve many other organs. Typically, WD diagnosis is established by biopsy of the small intestine. A case of WD, established by duodenal biopsy, in a 36‐yr‐old male is presented. This patient developed mesenteric lymphadenopathy, prompting computerized‐tomography guided fine‐needle aspiration (FNA), which showed typical features of WD. Electron microscopy (EM) studies confirmed the diagnosis. To our knowledge, this is the first reported case of lymph‐node involvement in WD diagnosed by FNA biopsy. © 1992 Wiley‐Liss, Inc.
Supportive role of image analysis and DNA ploidy pattern in the diagnosis of thyroid tumors
Authors: Kashyap V. , Kaushik N. , Bhambhani S. , Das D. K. , Luthra Usha K. 
Published: February 2007

AbstractTo evaluate the supportive role of image cytometry and DNA ploidy pattern in the diagnosis of thyroid tumors, a preliminary study was performed on fine needle aspirates of 30 cases. Of these, 10 cases each were of colloid goiter, follicular neoplasm, and papillary carcinoma. The nuclear area and DNA value of 50 cells in each case were measured. The mean nuclear area in colloid goiter (69.50 + 12.62 sq.μm) was significantly lower than the mean nuclear area in a follicular neoplasm (88.71 + 15.51 sq.μm) (P < 0.05). Similar differences between the mean nuclear area in colloid goiter and papillary carcinoma (124.0 + 12.27 sq μm) was also highly significant (P < 0.001). The results obtained by image cytometry were compared with estimated DNA ploidy pattern of follicular cells from the same cases. All colloid goiter had mean nuclear area below 100sq.μm with diploid DNA value. However, papillary carcinoma showed aneuploid DNA pattern in eight cases (80.0%), but mean nuclear area was above 100 sq. μm. A diagnostically useful finding obtained in two of the 10 cases of follicular neoplasm was the association of aneuploid DNA pattern with mean nuclear area of the follicular cells above 100 sq.μm. indicating a high probability of carcinoma and thus demanding an urgent open biopsy. These cases were readily distinguished from other cases of the same category showing diploid DNA pattern and mean nuclear area of follicular cells below 100 sq.μm. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of spindle cell neoplasms of the adrenal gland
Authors: Nance Keith V. , McLeod Darryl L. , Silverman Jan F. 
Published: February 2007

AbstractThree spindle cell neoplasms were encountered in a series of 46 FNA of the adrenal performed between 1984 and 1991. These neoplasms included a recurrent undifferentiated adrenal cortical carcinoma (ACC) with a predominant spindle cell pattern, a pheochromocytoma (PC), and a metastatic desmoplastic malignant melanoma (DMM), Cytologically, the ACC was characterized by the presence of numerous microtissue fragments composed of spindle‐shaped malignant cells with oval to spindle‐shaped nuclei, one or more nucleoli, and bipolar cytoplasmic processes. In some areas the tumor cells were dissected by vascular channels. The background contained abundant metachromatic stroma as well as individually scattered tumor cells. The PC was composed predominantly of loosely cohesive spindle‐shaped cells along with more polygonal shaped cells with delicate faintly staining granular cytoplasm. The tumor cells exhibited mild anisonucleosis. The tumor fragments were well vascularized by arborizing delicate capillary channels. The DMM was composed of microtissue fragments, interlacing fascicles and loose aggregates of spindle‐shaped malignant cells with hyperchromatic nuclei, small nucleoli, and an absence of cytoplasmic pigment. In each case ancillary studies including immunocytochemistry and electron microscopy (EM) were helpful in the differential diagnosis. The ACC was negative for cytokeratins, neuron‐specific enolase (NSE), and muscle‐specific actin (HHF), but displayed strong positivity for vimentin as welt as characteristic whorls of smooth endoplasmic reticulum by EM. The PC was positive for NSE and chromogranin with no EM performed. The DMM stained for S‐100 and vimentin but was negative for HMB‐45. cytokeratin, and HHF. EM examination revealed rare atypical premelanosomes. A discussion of spindle cell lesions of the adrenal gland encountered in FNA biopsy is presented along with the role of ancillary studies in the work‐up of these cases. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of ki‐1‐positive anaplastic large‐cell lymphoma
Authors: Akhtar Mohammed , Ali Muhammad Ashraf , Haider Abdulrazzaq , Antonius John , Hainau Bo , Dayel Fouad Al 
Published: February 2007

AbstractFive cases of Ki‐1‐positive anaplastic large‐cell lymphoma diagnosed by fine‐needle aspiration biopsy are reviewed, and cytologic, histologic, and ultrastructural findings in these cases are correlated. In all cases, the diagnosis of anaplastic large‐cell lymphoma was suggested on the basis of the morphological appearance in aspiration smears. This diagnosis was confirmed by immunohistochemistry, which revealed strong positivity of most of the cells by Ki‐1 antibody. Two of the lymphomas were T‐cell type, one was B‐cell type, and the remaining 2 were composed of null cells. In 2 cases, intracytoplasmic inclusions were seen in some of the tumor cells in aspiration smears. These were ultrastructurally correlated with large lysosomal bodies of variable morphology. Fine‐needle aspiration combined with immunohistochemistry may be an effective technique for diagnosing this neoplasm. © 1992 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of pediatric neoplasms: Correlation between electron microscopy and immunocytochemistry in diagnosis and classification
Authors: Akhtar Mohammed , Bedrossian Carlos W. M. , Ali Muhammad Ashraf , Bakry Mohammed 
Published: February 2007

AbstractA series of fine‐needle aspiration biopsies performed in 635 children were reviewed. The diagnoses rendered in these patients included malignant lymphoma in 139 (21.9%); Hodgkin 's disease, 25 (3.9%); neuroblastoma, 58 (9.1%); Wilms' Tumor, 37 (5.8%); Ewing's sarcoma, 32 (5.0%); rhabdomyosarcoma, 25 (3.9%); retinoblastoma, 22 (3.5%); leukemia infiltrate, 33 (5.2%); and miscellaneous tumors, 52 (8.2%). In 171 patients (26.9%), the biopsy was nondiagnostic. The cytomorphological characteristics of these lesions are briefly described and illustrated. Salient morphological features are further correlated with histological and ultrastructural appearances. Immunocytochemical patterns of these tumors are also discussed briefly. © 1992 Wiley‐Liss, Inc.