bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful anatomical closure of a photographically documented 30-year-old idiopathic full-thickness macular hole following surgery for concurrent repair of an acute macula-on rhematogenous retinal detachment
Authors: Au Eong Jonathan T W , Lim Jason H M , George Sachin M , Au Eong Kah-Guan 
Published: April 2024

Abstract
A 62-year-old man with a 30-year-old photographically documented idiopathic full-thickness macular hole and visual acuity of 6/45 developed an acute macula-on rhegmatogenous retinal detachment in his left eye. A pars plana vitrectomy, internal limiting membrane peeling around the macular hole, fluid-air exchange, endolaser retinopexy around the peripheral retinal break and perfluoropropane (C3F8) internal tamponade were performed to repair the detached retina and macular hole. One month postoperatively, the patient developed a large peripheral circumferential retinal tear with shallow retinal detachment which necessitated scleral buckling, repeat vitrectomy, endolaser photocoagulation and C3F8 tamponade. The retina was successfully re-attached and the macula hole was closed. Three years post-vitrectomy, the repaired 30-year-old macular hole remained closed although the visual acuity remained unchanged at 6/45. In summary, we describe the successful anatomical closure of a 30-year-old idiopathic full-thickness macular hole which we believe to be the longest duration photographically documented macular hole closed following surgery.
Bayesian Multisource Hierarchical Models with Applications to the Monthly Retail Trade Survey
Authors: Kaputa Stephen J , Morris Darcy Steeg , Holan Scott H 
Published: April 2024

Abstract
The integration of multiple survey, administrative, and third-party data offers the opportunity to innovate and improve survey estimation via statistical modeling. With decreasing response rates and increasing interest for more timely and geographically detailed estimates, imputation methodology that combines multiple data sources to adjust for low unit response and allow for more detailed publication levels, including geographic estimates, is both timely and necessary. Motivated by the Advance Monthly Retail Trade Survey (MARTS) and Monthly Retail Trade Survey (MRTS), we propose Bayesian hierarchical multiple imputation-dependent data models with the goals of automating imputation for the MARTS by using historic MRTS data and providing geographically granular (state-level) estimates for the MRTS via mass imputation using third-party data and spatial dependence. As a natural byproduct of this approach, measures of uncertainty are provided. This article illustrates the advantages of applying established Bayesian hierarchical modeling techniques with multiple source data to address practical problems in official statistics and is, therefore, of independent interest. The motivating empirical studies are unified by their hierarchical modeling framework, which ultimately results in a more principled approach for estimation for the MARTS and a more geographically granular data product for the MRTS.
Back to square one: the bodily roots of conscious experiences in early life
Authors: Ciaunica Anna , Safron Adam , Delafield-Butt Jonathan 
Published: April 2024

Abstract
Most theoretical and empirical discussions about the nature of consciousness are typically couched in a way that endorses a tacit adult-centric and vision-based perspective. This paper defends the idea that consciousness science may be put on a fruitful track for its next phase by examining the nature of subjective experiences through a bottom-up developmental lens. We draw attention to the intrinsic link between consciousness, experiences and experiencing subjects, which are first and foremost embodied and situated organisms essentially concerned with self-preservation within a precarious environment. Our paper suggests that in order to understand what consciousness ‘is’, one should first tackle the fundamental question: how do embodied experiences ‘arise’ from square one? We then highlight one key yet overlooked aspect of human consciousness studies, namely that the earliest and closest environment of an embodied experiencing subject is the body of another human experiencing subject. We present evidence speaking in favour of fairly sophisticated forms of early sensorimotor integration of bodily signals and self-generated actions already being established in utero. We conclude that these primitive and fundamentally relational and co-embodied roots of our early experiences may have a crucial impact on the way human beings consciously experience the self, body and the world across their lifespan.
Hepatitis C Cascade of Care in a Multidisciplinary Substance Use Bridge Clinic Model in Tennessee
Authors: Chirunomula Samantha , Muscarella Anahit , Whelchel Kristen , Gispen Fiona , Marcovitz David , White Katie , Chastain Cody 
Published: April 2024

Abstract
Many barriers prevent individuals with substance use disorders from receiving hepatitis C virus (HCV) treatment. This study describes 96 patients with active HCV treated in an opioid use disorder bridge clinic model. Of 33 patients who initiated treatment, 25 patients completed treatment, and 13 patients achieved sustained virologic response.
Managing Modern Antiretroviral Therapy in the Intensive Care Unit: Overcoming Challenges for Critically Ill People With Human Immunodeficiency Virus
Authors: Chastain Daniel B , Tu Patrick J , Brizzi Marisa , Keedy Chelsea A , Baker Aubrey N , Jackson Brittany T , Ladak Amber F , Hamilton Leslie A , Sells Nicholas R , Henao-Martínez Andrés F , McManus Kathleen A , Cluck David B 
Published: April 2024

Abstract
People with human immunodeficiency virus (HIV) have a 50% excess risk for intensive care unit (ICU) admission, often for non-HIV-related conditions. Despite this, clear guidance for managing antiretroviral therapy (ART) in this setting is lacking. Selecting appropriate ART in the ICU is complex due to drug interactions, absorption issues, and dosing adjustments. Continuing ART in the ICU can be challenging due to organ dysfunction, drug interactions, and formulary limitations. However, with careful consideration, continuation is often feasible through dose adjustments or alternative administration methods. Temporary discontinuation of ART may be beneficial depending on the clinical scenario. Clinicians should actively seek resources and support to mitigate adverse events and drug interactions in critically ill people with HIV. Navigating challenges in the ICU can optimize ART and improve care and outcomes for critically ill people with HIV. This review aims to identify strategies for addressing the challenges associated with the use of modern ART in the ICU.
Type I interferon promotes the fate of Toll-like receptor 9–stimulated follicular B cells to plasma cell differentiation
Authors: Higuchi Ryota , Tanaka Kaori , Saito Yuichi , Murakami Daisuke , Nakagawa Takashi , Nutt Stephen L , Ohkawa Yasuyuki , Baba Yoshihiro 
Published: April 2024

Abstract
The activation and differentiation of B cells into plasma cells (PCs) play critical roles in the immune response to infections and autoimmune diseases. Toll-like receptor 9 (TLR9) responds to bacterial and viral DNA containing unmethylated CpG motifs and triggers immune responses in B cells; however, abnormal recognition of self-DNA by TLR9 can cause autoimmune diseases. When stimulated with TLR9 agonists, follicular (FO) B cells, a subset of B cells residing in the FO regions of secondary lymphoid organs, exhibit a propensity for activation but fail to give rise to PCs. The factors that enable the transition of TLR9-activated FO B cells from activation to differentiation into PCs remain unclear. In this study, we show that type I interferon-alpha (IFNα) signaling causes FO B cells activated by CpG stimulation to differentiate into PCs. Although CpG stimulation alone only temporarily increased interferon regulatory factor 4 (IRF4) expression in FO B cells, co-stimulation with both CpG and IFNα enhanced and maintained high IRF4 expression levels, ultimately enabling the cells to differentiate into PCs. Overexpression of IRF4 in FO B cells results in CpG-induced PC transition without IFN signaling. Furthermore, co-stimulation of TLR9 and IFNα receptors significantly enhanced mammalian target of rapamycin (mTOR) signaling, which regulates IRF4 expression and PC generation. These findings suggest that IFNα may play a key role in promoting the fate of PC differentiation in FO B cells activated by TLR9 stimulation.
Current Auditory Hallucinations Are Not Associated With Specific White Matter Diffusion Alterations in Schizophrenia
Authors: Nerland Stener , Slapø Nora Berz , Barth Claudia , Mørch-Johnsen Lynn , Jørgensen Kjetil Nordbø , Beck Dani , Wortinger Laura A , Westlye Lars T , Jönsson Erik G , Andreassen Ole A , Maximov Ivan I , Geier Oliver M , Agartz Ingrid 
Published: April 2024

Abstract

Background and Hypothesis
Studies have linked auditory hallucinations (AH) in schizophrenia spectrum disorders (SCZ) to altered cerebral white matter microstructure within the language and auditory processing circuitry (LAPC). However, the specificity to the LAPC remains unclear. Here, we investigated the relationship between AH and DTI among patients with SCZ using diffusion tensor imaging (DTI).

Study Design
We included patients with SCZ with (AH+; n = 59) and without (AH−; n = 81) current AH, and 140 age- and sex-matched controls. Fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD) were extracted from 39 fiber tracts. We used principal component analysis (PCA) to identify general factors of variation across fiber tracts and DTI metrics. Regression models adjusted for sex, age, and age2 were used to compare tract-wise DTI metrics and PCA factors between AH+, AH−, and healthy controls and to assess associations with clinical characteristics.

Study Results
Widespread differences relative to controls were observed for MD and RD in patients without current AH. Only limited differences in 2 fiber tracts were observed between AH+ and controls. Unimodal PCA factors based on MD, RD, and AD, as well as multimodal PCA factors, differed significantly relative to controls for AH−, but not AH+. We did not find any significant associations between PCA factors and clinical characteristics.

Conclusions
Contrary to previous studies, DTI metrics differed mainly in patients without current AH compared to controls, indicating a widespread neuroanatomical distribution. This challenges the notion that altered DTI metrics within the LAPC is a specific feature underlying AH.
The NFκB Dif is required for behavioral and molecular correlates of sleep homeostasis in Drosophila
Authors: O’Hara Michael K , Saul Christopher , Handa Arun , Cho Bumsik , Zheng Xiangzhong , Sehgal Amita , Williams Julie A 
Published: April 2024

Abstract
The nuclear factor binding the κ light chain in B-cells (NFκB) is involved in a wide range of cellular processes including development, growth, innate immunity, and sleep. However, genetic studies of the role of specific NFκB transcription factors in sleep have been limited. Drosophila fruit flies carry three genes encoding NFκB transcription factors, Dorsal, Dorsal Immunity Factor (Dif), and Relish. We previously found that loss of the Relish gene from fat body suppressed daily nighttime sleep, and abolished infection-induced sleep. Here we show that Dif regulates daily sleep and recovery sleep following prolonged wakefulness. Mutants of Dif showed reduced daily sleep and suppressed recovery in response to sleep deprivation. Pan-neuronal knockdown of Dif strongly suppressed daily sleep, indicating that in contrast to Relish, Dif functions from the central nervous system to regulate sleep. Based on the unique expression pattern of a Dif- GAL4 driver, we hypothesized that its effects on sleep were mediated by the pars intercerebralis (PI). While RNAi knock-down of Dif in the PI reduced daily sleep, it had no effect on the recovery response to sleep deprivation. However, recovery sleep was suppressed when RNAi knock-down of Dif was distributed across a wider range of neurons. Induction of the nemuri (nur) antimicrobial peptide by sleep deprivation was reduced in Dif mutants and pan-neuronal overexpression of nur also suppressed the Dif mutant phenotype by significantly increasing sleep and reducing nighttime arousability. Together, these findings indicate that Dif functions from brain to target nemuri and to promote deep sleep.
Research progress on the therapeutic application of extracellular vesicles in erectile dysfunction
Authors: Zhang Xiaolin , Yang Mengbo , Chen Xinda , Lu Mujun 
Published: April 2024

Abstract
Erectile dysfunction (ED) is one of the most common male sexual dysfunctions and is related to many pathogenic factors. However, first-line treatment, represented by phosphodiesterase 5 inhibitors, is unable to maintain long-term efficacy. Extracellular vesicles (EVs) have recently attracted the attention of researchers in the fields of cardiovascular disease, neurologic disease, and regenerative medicine and may become a treatment for ED. This article reviews recent applications of EVs in the treatment of ED from the aspects of the source, the therapeutic mechanism, and the strategies to enhance therapeutic efficacy. These research advances lay the foundation for further research and provide references for in-depth understanding of the therapeutic mechanism and possible clinical application of EVs in ED.
Knocking down GRAMD1C expression reduces 6-OHDA-induced apoptosis in PC12 cells
Authors: He Hui , Zhang Bo , Wang Xiang , Chen Lulu 
Published: April 2024

Abstract

Aim
To explore the differential genes in Parkinson’s disease (PD) through a preliminary GEO database, and to investigate the possible mechanisms.

Materials and Methods
The PD differentially expressed genes (DEGs) were analyzed by the microarray method. Then, these DEGs were applied to KEGG and GO analyses to predict the related signaling pathways and molecular functions. Comparison of GRAMD1C expression levels in the putamen of normal and Parkinson’s patients by bioinformatic analysis. PC12 cells were cultured to construct a 6-hydroxydopamine (6-OHDA)-induced Parkinson’s cell model. RT-qPCR was performed to detect the efficiency of GRAMD1C siRNA. MTT assay was conducted to examine the proliferation of cells. Then, the apoptosis of each group of cells was measured by flow cytometry. Western blot was carried out to determine the expression of apoptosis-related proteins.

Results
Through bioinformatics, GRAMD1C was confirmed to be one of the most significantly upregulated genes in PD. Furthermore, GRAMD1C was notably enhanced in the PD patients and 6-OHDA-induced PC12 cells. Besides, 6-OHDA stimulation significantly reduced PC12 cell proliferation, and it reverted with the GRAMD1C siRNA. Moreover, the flow cytometry results showed that knockdown of GRAMD1C could effectively reduce the high apoptosis rate of PC12 cells induced by 6-OHDA treatment. Similarly, western blot results found that 6-OHDA stimulation markedly increased the expression levels of Bax and Caspase 3Caspase 3 and decreased the Bcl-2 expression in PC12 cells, and GRAMD1C knockdown reversed these changes.

Conclusion
GRAMD1C is upregulated in PD, and may affect the PD process through the apoptotic pathway.
Emergency medical service use at a pediatric center in Turkey, during the COVID-19 pandemic period
Authors: Bodur İlknur , Aydın Orkun , Yaradılmış Raziye Merve , Güneylioğlu Muhammed Mustafa , Güngör Ali , Akkaya Bilge , Göktuğ Aytaç , Öztürk Betül , Karacan Can Demir , Tuygun Nilden 
Published: April 2024

Abstract

Aim
This study aimed to evaluate aspects of pediatric patients presenting to a hospital in Turkey via emergency ambulance services, including incidence of visits to the hospital, acuity of illness and most common diagnoses, during the one-year period before and after the onset of the coronavrrus dsease 2019 (COVID-19) pandemic.

Methods
This was a retrospective and single center analysis of children, transported by Emergency Medical Services to the Emergency Department (ED) of a children's hospital in Turkey, between 10 March 2019 and 11 March 2021.

Results
Percentage of high acuity group (68.1% vs.76.9%) during pandemic period was significantly lower than prepandemic period (p &lt; 0.001). On the contrary, the percentage of patients using emergency ambulance service with a low level of acuity increased during the pandemic period compared to the prepandemic period (31.9% vs. 23.1%) (p &lt; 0.001). A significant decrease was observed in the cases of lower respiratory tract infections, febrile status epilepticus and excessive alcohol use during the pandemic period. No significant differences were found hospitalizations requiring PICU and mortality in ED during the pandemic period.

Conclusion
During the COVID-19 pandemic; also, a decrease in admissions was observed for those with high-risk conditions. On the contrary, an increase was detected in patients with low acuity levels. Efforts should be made to ensure access to safe and quality emergency care during the pandemic.
Deciphering Transcriptomic and Metabolomic Wood Responses to Grapevine Trunk Disease-Associated Fungi
Authors: Romeo-Oliván Ana , Chervin Justine , Breton Coralie , Puech-Pagès Virginie , Fournier Sylvie , Marti Guillaume , Rodrigues Olivier , Daydé Jean , Dumas Bernard , Jacques Alban 
Published: April 2024

Esca is one of the main grapevine trunk diseases affecting vineyards worldwide. Phaeoacremonium minimum and Phaeomoniella chlamydospora are thought to be two of the main causal agents of this disease. However, the molecular mechanisms underlying plant defense responses in the grapevine trunk against esca-associated pathogens are poorly understood. To provide a first glimpse at the trunk responses to P. minimum and P. chlamydospora, transcriptomic and metabolomic analyses were performed to compare and contrast host responses to these pathogens. Transcriptomic analysis revealed different gene expression reprogramming in the trunk in response to each fungus. The main significant differences were found among genes associated with secondary metabolism, signaling, and hormone signaling. An untargeted liquid chromatography–high resolution mass spectrometry metabolomic approach performed 3 weeks after inoculation was used, and dereplication mainly highlighted flavonoids and stilbenes as plant defense metabolites in the infected trunk. Some metabolites were overproduced with both fungi, but specific responses were also observed. Particularly, a lipophilic flavonoid cluster was emphasized after P. minimum inoculation. The assessment of fungal infection 6 weeks postinfection showed more copies of P. minimum than P. chlamydospora. This dissimilarity in the level of colonization could be linked to the metabolomic responses observed. Our results reveal both different gene expression reprogramming and metabolomic-specific signatures depending on the wood pathogen. Altogether, these observations suggest that grapevine trunks can differently perceive and respond to P. minimum and P. chlamydospora. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
SARS-CoV-2 Infection Among Newborn Infants
Authors: Mathew Liji , Schmolze Mia , Carter Kathleen V. 
Published: April 2024

Abtsract

Background:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection of mothers may increase the risk of complications and adverse birth outcomes among newborn infants born more than 37 weeks’ gestation.

Purpose:
The aim of this scoping review is to identify the research gaps in the literature on SARS-CoV-2 positive newborn infants born at more than 37 weeks’ gestation in United States (U.S.).

Data Sources:
A search for relevant articles was conducted using multiple resources including three databases CINAHL, Ovid MEDLINE, and Web of Science. This scoping review included case reports, case series, cohort, and retrospective studies focusing on newborn infants born more than 37 weeks of gestation with SARS-CoV-2 infection.

Study Selection:
A total of 4262 citations were screened, and 12 articles met the eligibility criteria.

Data Extraction:
Two authors independently screened the articles using a multi-step approach.

Results:
This review identified the gaps in literature on newborn infants up to one month of age. Few studies have focused on SARS-CoV-2 positive newborn infants born more than 37 gestational weeks. This review demonstrates a higher prevalence of community-acquired SARS-CoV-2 infections among infants following discharge.

Implications of Practice and Research:
Few U.S. based studies have focused on newborn infants born more than 37 weeks’ gestation with SARS-CoV-2 infection. Future follow-up studies are essential on these infants especially during the first 30 days of life. Discharge teaching on SARS-CoV-2 infection is vital in reducing community transmission, admissions, and emergency department visits.
Prevalence of Acetabular Dysplasia at a Mean age of 18 Years After Treatment for Neonatal hip Instability
Authors: Maikku Mari , Valkama Arja Marita , Ohtonen Pasi , Leppilahti Juhana 
Published: April 2024

Background:
The long-term radiological outcomes after the treatment of neonatal hip instability (NHI) in developmental dysplasia of the hip are unclear. Therefore, the prevalence of acetabular dysplasia at a mean age of 18 years after treatment was investigated. The relationship between acetabular dysplasia and hip discomfort has also been poorly established. Therefore, the differences in pain, hip-related quality of life (QOL), and hip impingement tests in hips with and without acetabular dysplasia were assessed.

Methods:
All 127 patients treated for NHI from 1995 to 2001 at the study hospital and meeting the inclusion criteria were invited to participate in this population-based follow-up. Of these individuals, 88 (69.3%) participated. The lateral center-edge angle (LCEA), Sharp’s angle (SA), and acetabular head index (AHI) were calculated for both hips from pelvic anterior–posterior radiographs. The Copenhagen Hip and Groin Outcome Score (HAGOS) questionnaire was completed for both hips separately; the total score, pain, and QOL subscores were calculated; and the impingement test was performed.

Results:
The prevalence of acetabular dysplasia, defined as an LCEA <20°, was 3.4%. Only the mean AHI (81.1%, SD 5.3) differed (-5.08, 95% CI -5.77 to -4.38, P <0.001) from previously described gender-specific and side-specific means, whereas the mean LCEA and SA did not. The odds ratio for a positive hip impingement test was 2.8 (95% CI: 1.11-7.05, P = 0.029) for hips with an LCEA <25° compared to hips with LCEA ≥25°. The hips with an SA ≥45° had a mean of 7.8 points lower for the HAGOS pain subscore (95% CI: 4.2-11.4, P <0.001) and a mean of 6.1 points lower for the HAGOS QOL subscore (95% CI: 2.1-10.2, P = 0.003) compared with hips with an SA <45°.

Conclusions:
The prevalence of acetabular dysplasia was low after treatment for NHI. Acetabular dysplasia seems to be as common in the general population as for those treated for NHI based on the LCEA. Only an SA ≥45° resulted in slightly more pain and lower hip-related QOL.