bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALISIS WACANA KRITIS MODEL TEUN A. VAN DIJK DALAM LIRIK LAGU â€œPARA PENCARI MUâ€ KARYA ENDA UNGU
Authors: Fadlan Zulbiyadi 
Published: March 2024

ABSTRAK: Wacana termasuk dalam unsur kebahasaan yang paling kompleks. Wacana yaitu unsur dari bahasa yang lebih memiliki sifat pragmatis dan pemakaian serta pemahaman wacana di dalam sebuah komunikasi memerlukan berbagai macam alat. Teun A. Van Dijk merupakan salah satu tokoh linguistik serta tokoh analisis wacana kritis yang model teorinya banyak digunakan analis wacana diseluruh dunia. Lagu â€œPara Pencari Muâ€ merupakan salah satu karya musik bergaya pop religi ciptaan Franco Wellijat Medjaya Kusumah dikenal dengan Enda Ungu yang berkembang di masyarakat Indonesia sejak tahun 2007. Lirik lagu â€œPara Pencari Muâ€ ini berisikan semangat hidup seseorang yang sedang mejalani hidup untuk pencarian yang indah pada tuhannya yaitu Allah SWT. Penelitian ini berfokus pada model teori Teun A. Van Dijk dan menganalisis struktur makro, menganalisis superstruktur, serta menganalisis konteks sosial yang terdapat pada lagu â€œPara Pencari Muâ€ ciptaan Enda ungu.
Kata kunci: Analisis Wacana Kritis, Lirik Lagu, Para Pencari Mu.
ABSTRACT: Discourse is one of the most complex linguistic elements. Discourse is an element of language that has a more pragmatic nature and the use and understanding of discourse in communication requires various kinds of tools. Teun A. Van Dijk is a linguistic figure and critical discourse analysis figure whose theoretical model is widely used by discourse analysts throughout the world. The song "Para Pencari Mu" is a work of religious pop style music created by Franco Wellijat Medjaya Kusumah, known as Enda Ungu, which has been developing in Indonesian society since 2007. The lyrics of the song "Para Pencari Mu" contain the spirit of life of someone who is living a life of search. beautiful in God, namely Allah SWT. This research focuses on Teun A. Van Dijk's theoretical model and analyzes the macro structure, analyzes the superstructure, and analyzes the social context contained in the song "Para Pencari Mu" created by Enda Ungu.
Keywords: Critical Discourse Analysis, Song Lyrics, Para Pencari Mu.
STRATEGI HUMAS POLRES CILEGON DALAM MENYOSIALISASIKAN PROGRAM BERMEDIA SOSIAL SECARA POSITIF DI LINGKUNGAN HUKUM POLRES CILEGON
Authors: Martha Marthalena , Hidayat Nina Ainul , Tri Santi Endang , Berthanila Rethorika 
Published: March 2024

ABSTRAK
Strategi humas Polres Cilegon dalam menyosialisasikan program â€œBermedia Sosial Secara Positifâ€ dilakukan hanya internal dengan melakukan face to face di lapangan Polres Cilegon. Penelitian ini bertujuan untuk mengetahui strategi humas yang dilakukan oleh humas Polres Cilegon dalam menyosialisasikan program â€œBermedia Sosial Secara Positifâ€ dengan melakukan fact finding, planning, communicating, evaluation dengan menggunakan teori Penyusunan Tindakan. Penelitian ini menggunakan pendekatan kualitatif dengan metode studi kasus yang didapat melalui hasil pemaparan dan penjelasan berdasarkan wawancara dan observasi dilapangan. Hasil dalam menyosialisasikan program â€œBermedia Sosial Secara Positifâ€ ini humas Polres Cilegon mendapatkan respon yang positif dari anggota Polres Cilegon dalam melakukan sosialisasi juga humas Polres membuat pemberitaan melalui website resmi Polres Cilegon, media lokal, dan Instagram resmi Polres Cilegon untuk menginformasikan bahwa di Polres Cilegon sedang melakukan Program sosialisasi

Kata Kunci : menyosialisasikan, Humas, strategi Humas,
Potensi Penyimpanan Karbon pada Lamun Thalassia Hempricii di Perairan Tanggetada, Kabupaten Kolaka
Authors: Antariksa Tasabaramo Ilham , Riska  , Laode Abdul Fajar Hasidu  , Aditya Hikmat Nugraha  , Hasan Eldin  Adimu  , Maharani  , Putri Cahyani  
Published: March 2024

Thalassia hempricii merupakan lamun yang memiliki sebaran dan kerapatan yang tinggi di perairan Kecamatan Tanggetada. Beberapa studi mengatakan, lamun ini memiliki kontribusi yang besar terhadap penyerapan karbon di atmosfer. Oleh karena itu, perlu dilakukan penelitian mengenai potensi penyimpanan karbon pada lamun Thalassia Hempricii. Tujuan dari penelitian ini adalah mengetahui seberapa besar lamun Thalassia Hempricii menyimpan karbon, baik pada bagian atas lamun maupun bagian bawah lamun. Metode yang digunakan untuk menghitung karbon adalah dengan metode faktor konversi biomassa ke karbon. Lokasi penelitian dilakukan di 3 stasiun yaitu Kelurahan Tanggetada, Desa Palewai dan Kelurahan Anaiwoi. Hasil penelitian menunukkan bahwa kandungan karbon yang tersimpan pada lamun Thalassia hempricii adalah sebesar 362,54 gC/m2. Karbon yang tersimpan pada lamun bagian bawah lebih besar dibandingkan lamun bagian atas. Karbon yang tersimpan pada lamun bagian bawah berkisar antara 79, 60-497 gC/m2 dan pada lamun bagian atas berkisar antara 17,50-131,43 gC/m2.
DoE-based medium optimization for improved biosurfactant production with Aureobasidium pullulans
Authors: Haala Frederick , Dielentheis-Frenken Marie R. E. , Brandt Friedrich M. , Karmainski Tobias , Blank Lars M. , Tiso Till 
Published: March 2024

Polyol lipids (a.k.a. liamocins) produced by the polyextremotolerant, yeast-like fungus Aureobasidium pullulans are amphiphilic molecules with high potential to serve as biosurfactants. So far, cultivations of A. pullulans have been performed in media with complex components, which complicates further process optimization due to their undefined composition. In this study, we developed and optimized a minimal medium, focusing on biosurfactant production. Firstly, we replaced yeast extract and peptone in the best-performing polyol lipid production medium to date with a vitamin solution, a trace-element solution, and a nitrogen source. We employed a design of experiments approach with a factor screening using a two-level-factorial design, followed by a central composite design. The polyol lipid titer was increased by 56% to 48 g L−1, and the space-time yield from 0.13 to 0.20 g L−1 h−1 in microtiter plate cultivations. This was followed by a successful transfer to a 1 L bioreactor, reaching a polyol lipid concentration of 41 g L−1. The final minimal medium allows the investigation of alternative carbon sources and the metabolic pathways involved, to pinpoint targets for genetic modifications. The results are discussed in the context of the industrial applicability of this robust and versatile fungus.
Validating the inactivation of viral pathogens with a focus on SARS-CoV-2 to safely transfer samples from high-containment laboratories
Authors: Chaki Sankar Prasad , Kahl-McDonagh Melissa M. , Neuman Benjamin W. , Zuelke Kurt A. 
Published: March 2024

IntroductionPathogen leak from a high-containment laboratory seriously threatens human safety, animal welfare, and environmental security. Transportation of pathogens from a higher (BSL4 or BSL3) to a lower (BSL2) containment laboratory for downstream experimentation requires complete pathogen inactivation. Validation of pathogen inactivation is necessary to ensure safety during transportation. This study established a validation strategy for virus inactivation. MethodsSARS-CoV-2 wild type, delta, and omicron variants underwent heat treatment at 95°C for 10 minutes using either a hot water bath or a thermocycler. To validate the inactivation process, heat-treated viruses, and untreated control samples were incubated with A549-hACE2 and Vero E6-TMPRSS2-T2A-ACE2 cells. The cells were monitored for up to 72 hours for any cytopathic effects, visually and under a microscope, and for virus genome replication via RT-qPCR. The quality of post-treated samples was assessed for suitability in downstream molecular testing applications. ResultsHeat treatment at 95°C for 10 minutes effectively inactivated SARS-CoV-2 variants. The absence of cytopathic effects, coupled with the inability of virus genome replication, validated the efficacy of the inactivation process. Furthermore, the heat-treated samples proved to be qualified for COVID-19 antigen testing, RT-qPCR, and whole-genome sequencing. DiscussionBy ensuring the safety of sample transportation for downstream experimentation, this validation approach enhances biosecurity measures. Considerations for potential limitations, comparisons with existing inactivation methods, and broader implications of the findings are discussed.
Clinical significance of regional constructive and wasted work in patients receiving cardiac resynchronization therapy
Authors: Wang Chun-Li , Wu Lung-Sheng , Wu Chia-Tung , Yeh Yung-Hsin , Cheng Yu-Wen , Yen Kun-Chi , Chan Yi-Hsin , Chuang Chi , Kuo Chi-Tai , Chu Pao-Hsien 
Published: March 2024

BackgroundPrevious studies have shown that global constructive work (CW) and wasted work (WW) predict response to cardiac resynchronization therapy (CRT). This study evaluated the predictive value of regional CW and WW for reverse remodeling and clinical outcomes after CRT.MethodsWe performed a prospective study involving 134 CRT candidates with left bundle branch block and left ventricular ejection fraction ≤35%. Global and regional CW and WW were calculated using pressure-strain loop analysis. CRT response was defined by reverse remodeling as a reduction of ≥15% in left ventricular end-systolic volume after six months.ResultsAt six-month follow-up, 92 (69%) patients responded to CRT. Of the regional CW and WW measures, lateral wall (LW) CW and septal WW were most strongly and significantly correlated with reverse remodeling. At multivariate analysis, LW CW and septal WW were both independent determinants of reverse remodeling. When LW CW and septal WW were included in the model, global CW and WW were not independently associated with reverse remodeling. LW CW and septal WW predicted reverse remodeling with an area under the curve (AUC) of 0.783 (95% CI: 0.700–0.866) and 0.737 (95% CI: 0.644–0.831), respectively. Using both variables increased the AUC to 0.832 (95% CI: 0.755–0.908). Both LW CW ≤878 mmHg% (HR 2.01; 95% CI: 1.07–3.79) and septal WW ≤181 mmHg% (HR 2.60; 95% CI: 1.38–4.90) were significant predictors of combined death and HF hospitalization at two-year follow-up.ConclusionLW CW and septal WW before CRT are important determinants of reverse remodeling and clinical outcomes.
Composition and diversity of rhizosphere microorganisms of Suaeda salsa in the Yellow River Delta
Authors: Xu Hui , Li Na , Li Wenjuan , Wang Hongguo , Shao Yan , Liu Jingmei , Zhang Jiabo , Wang Jun , Shang Shuai 
Published: March 2024

IntroductionSuaeda salsa is a typical wetland plant species in coastal areas that plays an important role in protecting the marine eco-environment. The rhizosphere microorganisms of S. salsa are responsible for its growth and development.MethodEighteen samples were collected from three areas, including the natural S. salsa-growing area (YDJ), artificial S. salsa restoration area (YDB), and nonrestoration area (BKS), and high-throughput sequencing technology was employed to explore the characteristics of the rhizosphere microorganisms of S. salsa in the Yellow River Delta.ResultsThe results illustrated that the abundance and diversity of soil bacteria were highest in the YDJ group, fungal abundance was highest in the YDJ group, and fungal diversity was greatest in the YDB group. In total, 26,663 operational taxonomy units (OTUs) were found in soil bacteria, among which 9,095, 8,023, and 11,001 were detected in the BKS, YDB, and YDJ groups, respectively. 11,619 OTUs were found in soil fungi, among which 4,278, 4,552, and 5,100 were detected in the BKS, YDB, and YDJ groups, respectively. The YDJ group had the highest number of OTUs for bacteria and fungi among the three groups.DiscussionS. salsa in natural wetland conditions tended to be similar to artificially restored S. salsa. The composition of fungi in the S. salsa rhizosphere had greater similarities than that of the bacteria. Proteobacteria had the highest abundance among bacterial communities, and Ascomycota, Basidiomycota, and Olpidiomycota were dominant in the fungal communities of the three groups. The correlation results found that power of hydrogen (pH) was significantly and negatively correlated with the abundance of Acidobacteriota and Proteobacteria. Meanwhile, electrical conductivity (EC) was significantly and positively correlated with the abundance of Firmicutes and negatively correlated with that of Proteobacteria. Regarding fungi, pH and EC were significantly and negatively correlated with the abundance of Chytridiomycota. Our findings provided some theoretical data for S. salsa conservation and wetland restoration.
Shrubs plays an important role in configuration of shelterbelt in windy and sandy areas
Authors: Zhang Shuai , Yuan Weijie , Yu Yi , Zhang Yaoxiang , Wang Weinan , Wang Luzhen , Yang Ying , Wang Haixia 
Published: March 2024

IntroductionPlanted forests are of great significance in reducing wind erosion and controlling degraded land, and are the main measure to improve the ecological environment in arid and semi-arid areas. Afforestation is mainly based on tall trees, but forest belts consume a large amount of water, bringing significant pressure to the environmental carrying capacity. While shrubs generally consume less water than do trees, it remains understudied what role shrubs play in configuration of shelterbelts in arid areas.MethodsIn this study, we conducted wind tunnel simulation experiments with the common used afforestation tree species Populus popularis and shrub species Salix psammophila. We set up single- or double-belt pure forest and mixed tree/shrub configurations, to analyze their effects on the wind speed, flow field, and wind prevention efficiency of different forest belt types.ResultsThe results showed that: 1) the wind erosion resistance of the double-belt shelter forest was stronger than that of a single-belt forest. 2) Shelterbelts consisting of trees had a strong resistance to airflow disturbances, but their protective effect was reflected mainly in the canopy layer, with poor near-surface protection. 3) Shelterbelts consisting of shrubs had a good protective effect within effective height, and the wind speed in the double-shrub forest belt was 77% of that in the single-tree forest belt. 4) The wind prevention efficiency within the shrub forest belt was better than that of the tree forest belt, with wind prevention efficiency exceeding 40% in most double-shrub forests. Shrubs as a supplement to trees could increase the minimum wind prevention efficiency by up to 30%.DiscussionTherefore, the role of shrubs should be emphasized in afforestation in arid areas, especially in areas that need near-surface protection.
Tree growth at the limits: the response of multiple conifers to opposing climatic constraints along an elevational gradient in the Alps
Authors: Obojes Nikolaus , Buscarini Serena , Meurer Armin Konrad , Tasser Erich , Oberhuber Walter , Mayr Stefan , Tappeiner Ulrike 
Published: March 2024

IntroductionClimate change affects the vitality of mountain forests through increasing temperatures and decreasing water availability due to changing precipitation patterns, earlier snowmelt, and increasing evaporative demand. Depending on species characteristics, tree growth might therefore increase in cold habitats near the forest line but decrease in water-limited conditions at low elevation.MethodsWe analyzed the tree-ring widths of five conifers (Picea abies, Larix decidua, Pinus sylvestris, Pinus nigra, and Pinus cembra) along an elevational gradient from 1,000 m to 2,320 m above sea level (a.s.l.) in Vinschgau/Val Venosta Valley in Northern Italy, one of the driest regions of the Alps (mean annual precipitation of 682 mm at 1,310 m a.s.l.).ResultsOur aim was to estimate the species-specific growth response to changing climate conditions along an elevational gradient. At low elevations, we observed a significant response to water availability not only during the actual growing season but also throughout the previous autumn for all species present. At mid-elevation, the correlation coefficients to precipitation and drought indices (SPEI) were highest for Picea abies. At high elevations, the positive correlation of growth with temperature was smaller than expected for Pinus cembra. In contrast, Larix decidua responded positively to temperature and grew faster in recent decades.DiscussionConsidering that a further increase in temperatures will reduce plant water availability during the growing season, our space-for-time approach provides an outlook on future growth conditions of conifers in larger regions of the European Alps. Water limitation will affect tree growth and vitality not only at low elevation in the valleys but also at mid elevation on mountain slopes, potentially impacting timber production and protective and recreative functions of forests. Near the forest line, the different capabilities of tree species to benefit from higher temperatures might lead to changes in species composition.
Rapid isolation of pan-neutralizing antibodies against Omicron variants from convalescent individuals infected with SARS-CoV-2
Authors: Yu Peng , Ran Jingping , Yang Ruiqi , Zhu Hang , Lu Song , Wu Yuzhang , Zhao Tingting , Xiong Tianchen 
Published: March 2024

IntroductionThe emergence of SARS-CoV-2 Omicron subvariants has presented a significant challenge to global health, as these variants show resistance to most antibodies developed early in the pandemic. Therapeutic antibodies with potent efficacy to the Omicron variants are urgently demanded.MethodsUtilizing the rapid antibody discovery platform, Berkeley Lights Beacon, we isolated two monoclonal neutralizing antibodies, 2173-A6 and 3462-A4. These antibodies were isolated from individuals who recently recovered from Omicron infections.ResultsBoth antibodies, 2173-A6 and 3462-A4, demonstrated high affinity for the RBD and effectively neutralized pseudoviruses from various Omicron lineages, including BA.4/5, XBB.1.16, XBB.1.5, and EG.5.1. This neutralization was achieved through binding to identical or overlapping epitopes.DiscussionThe use of the Beacon platform enabled the rapid isolation and identification of effective neutralizing antibodies within less than 10 days. This process significantly accelerates the development of novel therapeutic antibodies, potentially reducing the time required to respond to unknown infectious diseases in the future.
The effect of contextual factors on a health intervention against podoconiosis in Ethiopia
Authors: Engdawork Kibur , Tadele Getnet , Nahar Papreen , Davey Gail , Zaman Shahaduz 
Published: March 2024

IntroductionUnderstanding contextual factors that influence the implementation and outcome of interventions is crucial to improving them. Outcome and process evaluation studies have to date focused on the successes and limitations of interventions with little consideration of external factors that could either facilitate or impede them. Guided by the Context and Implementation of Complex Intervention (CICI) framework, we evaluated the effect of contextual factors on a health intervention implemented against the neglected tropical disease (NTD) podoconiosis in Ethiopia.MethodWe purposefully selected a health intervention jointly implemented by two NGOs in podoconiosis-endemic districts in Northwestern Ethiopia. We employed an exploratory mixed methods approach, and data were collected between April and July 2022. We first conducted document analysis, observations, 4 focus group discussions, 32 in-depth interviews and 19 key informant interviews. Subsequently, we administered a survey to 369 rural residents, of whom 42 were affected by podoconiosis. The qualitative data were managed with NVivo version 12 software. The quantitative data were analyzed using Stata version 15 software.ResultsMost project activities were implemented as per the initial plan and the intervention reached patients that never had access to treatment services before. Contextual factors (geographical, epidemiological, socio-economic, socio-cultural, ethical, legal and political) and lived experience of patients influenced the implementation and outcome of the intervention. Most of the contextual factors pose constraints on implementation and affected the acceptability and sustainability of project activities. On the other hand, political context such the commitment of the government to rollout national programs and set up NTD structures at district level positively influenced the implementation of the intervention.ConclusionHealth intervention implementors should be aware of the interactive effects of contextual factors wherein interventions are put into practice. Minimizing the constraining effects of these contextual factors while utilizing opportunities presented by them might help implement optimal strategies to improve the lives of individuals affected by podoconiosis and similar NTDs.
Evaluation of serum and urine biomarkers for severe COVID-19
Authors: Shansky Yaroslav D. , Yanushevich Oleg O. , Gospodarik Alina V. , Maev Igor V. , Krikheli Natella I. , Levchenko Oleg V. , Zaborovsky Andrew V. , Evdokimov Vladimir V. , Solodov Alexander A. , Bely Petr A. , Andreev Dmitry N. , Serkina Anna N. , Esiev Sulejman S. , Komarova Anastacia V. , Sokolov Philip S. , Fomenko Aleksei K. , Devkota Mikhail K. , Tsaregorodtsev Sergei V. , Bespyatykh Julia A. 
Published: March 2024

IntroductionThe new coronavirus disease, COVID-19, poses complex challenges exacerbated by several factors, with respiratory tissue lesions being notably significant among them. Consequently, there is a pressing need to identify informative biological markers that can indicate the severity of the disease. Several studies have highlighted the involvement of proteins such as APOA1, XPNPEP2, ORP150, CUBN, HCII, and CREB3L3 in these respiratory tissue lesions. However, there is a lack of information regarding antibodies to these proteins in the human body, which could potentially serve as valuable diagnostic markers for COVID-19. Simultaneously, it is relevant to select biological fluids that can be obtained without invasive procedures. Urine is one such fluid, but its effect on clinical laboratory analysis is not yet fully understood due to lack of study on its composition.MethodsMethods used in this study are as follows: total serum protein analysis; ELISA on moderate and severe COVID-19 patients’ serum and urine; bioinformatic methods: ROC analysis, PCA, SVM.Results and discussionThe levels of antiAPOA1, antiXPNPEP2, antiORP150, antiCUBN, antiHCII, and antiCREB3L3 exhibit gradual fluctuations ranging from moderate to severe in both the serum and urine of COVID-19 patients. However, the diagnostic value of individual anti-protein antibodies is low, in both blood serum and urine. On the contrary, joint detection of these antibodies in patients’ serum significantly increases the diagnostic value as demonstrated by the results of principal component analysis (PCA) and support vector machine (SVM). The non-linear regression model achieved an accuracy of 0.833. Furthermore, PCA aided in identifying serum protein markers that have the greatest impact on patient group discrimination. The study revealed that serum serves as a superior analyte for describing protein quantification due to its consistent composition and lack of organic salts and drug residues, which can otherwise affect protein stability.