bluuebunny/crossref_metadata_2025_split huggingface dataset:
Company Life Cycle, Merger Activities in Indonesia
Authors: Andriyani Ima , Yuliani Yuliani , Marzuki Agustina 
Published: March 2024

This study aims to explain and analyze the influence of the company's life cycle on the probability of conducting mergers, shareholder wealth, percentage of company stock ownership and payment methods by mergers . The sample of the research is 36 companies that have merged and acquired in Indonesia which are listed on the Indonesia Stock Exchange (BEI) in the period 2018-2022. Data analysis uses logistic regression and multiple linear regression methods . The results showed the company's life cycle did not significantly affect the probability of companies doing mergers . The company's life cycle has a significant effect on shareholder wealth. the company's life cycle has no significant effect on the percentage of share ownership , the company's life cycle has a positive and significant effect on payment methods for companies that have made mergers . The theoretical implications of this study provide an overview / explanation of the company's life cycle of the activities of mergers in company policy seen from the company's growth prospects. Keywords: Company life cycle, Merger Activities
Pelaksanaan Kampus Merdeka Angkatan 1 Program Merdeka Belajar Kampus Merdeka Kegiatan Asistensi Mengajar Di Smk Negeri 1 Belinyu
Authors: Luthfiana Nur , Yusnita Maya 
Published: March 2024

The implementation of the Merdeka Belajar - Kampus Merdeka (MBKM) Activities by the Ministry of Education and Culture of the Republic of Indonesia Batch 1 (first) provides opportunities for students to choose various Merdeka Belajar – Kampus Merdeka (MBKM) programs consisting of eight programs offered, namely: (1) Building a Thematic Village/KKN; (2) Apprenticeship; (3) Entrepreneurship; (4) Humanitarian Projects; (5) Independent Study/Project; (6) Student Research; (7) Student Exchange; and (8) Teaching Assistance. The current program is Teaching Assistance, which provides opportunities for students to add insight and gain experience in assisting the teaching and learning process in the Vocational High School Education unit. Learning is carried out using offline and online methods; there is offline or face-to-face learning and online learning because it still suppresses the increasing number of COVID-19 cases. The program implemented includes carrying out the teaching and learning process, training for all teachers and activities for students
Riscos & Desafios (Risk & Challenges) Program in pandemic times: the students’ voice
Authors: Santos Maria Paula , Cardoso Gizela , Silva Paula , Rocha Carla 
Published: March 2024

Background: Preventive interventions are important to delay the onset of high-risk behaviors for young people's health. Regarding emerging adulthood, successful preventive programs are important to avoid high-risk alcohol consumption among university students. Riscos & Desafios (Risk & Challenges) Program is an extracurricular, comprehensive, and multi-component competence enhancement-based preventive intervention designed for students attending the first year of higher education. Goals: This paper aims to give voice to the procedural assessment made by students and to describe the implementation of the Program at the Faculty of Sport of the University of Porto in the second semester of the 2019/2020 school year. This implementation of a previous validated program was innovative twofold, since it has a curriculum infusion format through an optional curricular unit, and because it was developed during the period of lockdown due to COVID-19 pandemic.Methods: This is a mixed-method study that focus on the implementation of the Riscos & Desafios Program during the second semester of 2019/20 academic year. This evaluation of the Riscos & Desafios Program was done with a post-program survey, followed by univariate analysis of frequency. Complementary, an inductive analysis of qualitative data of student’s reflexive reports was carried out, using the QSR-NVIVO 12®.Results: Twenty-three first-year students who participated in the Riscos & Desafios Program constituted the target group of the intervention. Most of the participants were male (n=16) aging between 17 to 21 years. Most attended all the eight sessions and 96% of them participated in seven or more sessions. At the end of the implementation, 23 post-program filled-in questionnaires, and 18 structured reflection reports, were obtained. Most of the students rated the program as very good. Several categories emerged from the thematic analysis of the 18 reports, namely (1) perceived competencies, developed in the program, including the promotion of the social support network, interpersonal and communication skills, problem-solving skills, and their importance, as factors to protect against the development of risk behaviors, (2) personal positive impacts of the program attendance and (3) gains and losses with the transition to online learning format.Discussion: Students’ considerations about the experience with the Riscos & Desafios Program not only reinforced the importance and pertinence of this experience in their life trajectory, but also brought to light its relevance at this pandemic moment.
UPAYA MENINGKATKAN MOTIVASI BELAJAR PADA ANAK SDN 120 INPRES TAMALATE, GALESONG UTARA, TAKALAR, SULAWESI SELATAN
Authors: Marian Fungky , Yansyah Medi , Hesti Hesti , Gusrini Indah Nurlisa 
Published: March 2024

Pendidikan di desa Tamalate berjalan dengan baik, namun masih kekurangan jumlah tenaga pendidik. Para siswa juga putus sekolah akibat kurangnya motivasi belajar. Banyak siswa memilih untuk bekerja sebagai nelayan setelah lulus SD atau melakukan pernikahan dini. Permasalahan tersebut diatasi menggunakan metode pemberian permainan edukatif berupa permainan brain gym untuk menumbuhkan motivasi belajar pada anak. Kegiatan permainan brain gym dilakukan sebelum dan sesudah pemberian materi pelajaran. Diharapkan dengan adanya kegiatan ini dapat menumbuhkan motivasi belajar pada siswa SDN 120 Inpres Tamalate agar dapat melanjutkan pendidikanya ke jenjang yang lebih tinggi. Peran aktif dari pemerintah atau dinas pendidikan juga dibutuhkan untuk dapat menambah jumlah guru di SDN 120 Inpres Tamalate serta dapat dilakukan sosialisasi lanjutan mengenai pentingnya pendidikan dan penanggulangan pernikahan dini.
Synthesis under high pressure: crystal structure and properties of cubic Dy36O11F50[AsO3]12 ∙ H2O
Authors: Goerigk Felix Christian , Locke Ralf Jules Christian , Schleid Thomas 
Published: March 2024

The multi-anionic compound with the composition Dy36O11F50[AsO3]12 ∙ H2O, which can be described in the non-centrosymmetric cubic space group F4¯3c, already shows an unusually large unit cell with an axis of a = 2587.59(14) pm. Its crystal structure exhibits isolated ψ1-tetrahedral [AsO3]3– anions, but both the coordination numbers and the linking schemes of the Dy3+-centered polyhedra differ significantly from the mostly layered structures described so far in literature. (Dy1)3+ is sevenfold coordinated by oxygen atoms and F− anions, forming a capped trigonal prism [(Dy1)O4.333F2.667]8.333–, and the remaining two cations (Dy2)3+ and (Dy3)3+ both reside in an eightfold coordination of anions. In both cases they form slightly distorted square antiprisms, which have the compositions of [(Dy2)O3.667F4.333]8.667– and [(Dy3)O4.667F3.333]9.667–, respectively. Some of the oxygen atoms are not part of ψ1-[AsO3]3– tetrahedra, but occur as O2– anions and one of these even shares a common crystallographic position with fluoride (F−). It is also worth mentioning that the single crystals were obtained as comparatively large cubes with an edge length of several 100 µm providing very good data with regard to single-crystal X-ray diffraction. To verify the simultaneous presence of oxygen and fluorine, electron-beam microprobe analysis was carried out, and a single-crystal Raman spectrum ruled out the presence of hydroxide anions or protonated [AsO3]3– groups, but proved the interstitial crystal-water molecules, which could not be determined precisely by the crystal-structure refinement.
HemU and TonB1 contribute to hemin acquisition in Stenotrophomonas maltophilia
Authors: Liao Chun-Hsing , Lu Hsu-Feng , Yang Ching-Wei , Yeh Ting-Yu , Lin Yi-Tsung , Yang Tsuey-Ching 
Published: March 2024

IntroductionThe hemin acquisition system is composed of an outer membrane TonB-dependent transporter that internalizes hemin into the periplasm, periplasmic hemin-binding proteins to shuttle hemin, an inner membrane transporter that transports hemin into the cytoplasm, and cytoplasmic heme oxygenase to release iron. Fur and HemP are two known regulators involved in the regulation of hemin acquisition. The hemin acquisition system of Stenotrophomonas maltophilia is poorly understood, with the exception of HemA as a TonB-dependent transporter for hemin uptake.MethodsPutative candidates responsible for hemin acquisition were selected via a homolog search and a whole-genome survey of S. maltophilia. Operon verification was performed by reverse transcription-polymerase chain reaction. The involvement of candidate genes in hemin acquisition was assessed using an in-frame deletion mutant construct and iron utilization assays. The transcript levels of candidate genes were determined using quantitative polymerase chain reaction.ResultsSmlt3896-hemU-exbB2-exbD2-tonB2 and tonB1-exbB1-exbD1a-exbD1b operons were selected as candidates for hemin acquisition. Compared with the parental strain, hemU and tonB1 mutants displayed a defect in their ability to use hemin as the sole iron source for growth. However, hemin utilization by the Smlt3896 and tonB2 mutants was comparable to that of the parental strain. HemA expression was repressed by Fur in iron-replete conditions and derepressed in iron-depleted conditions. HemP negatively regulated hemA expression. Like hemA, hemU was repressed by Fur in iron-replete conditions; however, hemU was moderately derepressed in response to iron-depleted stress and fully derepressed when hemin was present. Unlike hemA and hemU, the TonB1-exbB1-exbD1a-exbD1b operon was constitutively expressed, regardless of the iron level or the presence of hemin, and Fur and HemP had no influence on its expression.ConclusionHemA, HemU, and TonB1 contribute to hemin acquisition in S. maltophilia. Fur represses the expression of hemA and hemU in iron-replete conditions. HemA expression is regulated by low iron levels, and HemP acts as a negative regulator of this regulatory circuit. HemU expression is regulated by low iron and hemin levels in a hemP-dependent manner.
The effect of exercise on flow-mediated dilation in people with type 2 diabetes mellitus: a systematic review and meta-analysis of randomized controlled trials
Authors: Qiu Bopeng , Zhou Yilun , Tao Xifeng , Hou Xiao , Du Liwen , Lv Yuanyuan , Yu Laikang 
Published: March 2024

IntroductionAn increasing number of studies have investigated the effect of exercise on flow-mediated dilation (FMD) in people with type 2 diabetes mellitus (T2DM), while the findings were controversial. The primary aim of this systematic review and meta-analysis was to investigate the effect of exercise on FMD in T2DM patients, and the secondary aim was to investigate the optimal type, frequency, session duration, and weekly time of exercise for T2DM patients.MethodsSearches were conducted in PubMed, Cochrane Library, Scopus, Web of Science, Embase and EBSCO databases. The Cochrane risk of bias tool (RoB2) in randomized trial and Physiotherapy Evidence Database (PEDro) scale were used to assess the methodological quality of the included studies.ResultsFrom the 3636 search records initially retrieved, 13 studies met the inclusion criteria. Our meta-analysis revealed that exercise had a significant effect on improving FMD in T2DM patients [WMD, 2.18 (95% CI, 1.78-2.58), p &lt; 0.00001, I2 = 38%], with high-intensity interval training (HIIT) being the most effective intervention type [HIIT, 2.62 (1.42-3.82); p &lt; 0.0001; aerobic exercise, 2.20 (1.29-3.11), p &lt; 0.00001; resistance exercise, 1.91 (0.01-3.82), p = 0.05; multicomponent training, 1.49 (0.15-2.83), p = 0.03]. In addition, a higher frequency [&gt; 3 times, 3.06 (1.94-4.19), p &lt; 0.00001; ≤ 3 times, 2.02 (1.59-2.45), p &lt; 0.00001], a shorter session duration [&lt; 60 min, 3.39 (2.07-4.71), p &lt; 0.00001; ≥ 60 min, 1.86 (1.32-2.40), p &lt; 0.00001], and a shorter weekly time [≤ 180 min, 2.40 (1.63-3.17), p &lt; 0.00001; &gt; 180 min, 2.11 (0.82-3.40), p = 0.001] were associated with larger improvements in FMD.ConclusionThis meta-analysis provides clinicians with evidence to recommended that T2DM patients participate in exercise, especially HIIT, more than 3 times per week for less than 60 min, with a target of 180 min per week being reached by increasing the frequency of exercise.Systematic review registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42023466575.
Research on performance of jet refrigeration cycle driven by waste heat of refrigerated vehicle
Authors: Jiang Shujun , Pan Xiubin , Zhang Song , Huang Yanmei , Que Wenshuai , Sang Hailang , Guan Wei 
Published: March 2024

Nowadays, refrigerated food storage and transport vehicles are very common. However, with regard to the engine of vehicles, a significant amount of heat is discharged into the environment in the form of exhaust gas and jacket water. If the cooling system is used to convert this waste heat into cooling capacity, the thermal efficiency of the engine will be improved to a certain extent. Therefore, this paper uses two waste heat recovery jet refrigeration systems to recover heat from the exhaust gas and jacket water of the refrigerated vehicle. One is a conventional jet refrigeration system that uses only engine exhaust as the heat source, and the other is a system that adds a preheater upstream of the generator based on the conventional system, using engine jacket water to preheat the working fluid. This paper presents the results of comparing the impact of R141b/R123, R141b/R245fa, R142b/R134a, and R142b/R152a working fluids on system performance, and considers the effects of operating temperature and secondary flow pressure drop in the ejector receiving chamber on the system performance. The research results indicate that of the four selected working fluids, the R141b/R245fa (0.4/0.6) blend has the best overall performance. After the addition of a preheater, the system using R142b/R134a (0.6/0.4) hybrid working fluid achieves the maximum cooling capacity of 16.0994 kW and the maximum thermal efficiency of 5.45%, and the exergy loss in the generator is 1 kW lower than without the preheater.
Experiences of young mothers with the uptake of Sulfadoxine-Pyrimethamine for intermittent preventive treatment of malaria in pregnancy: a cross-sectional study in the Lake endemic region, Kenya
Authors: Matanda Dennis Juma , Zulu Tchaiwe , Odwe George , Okoth Oscar , Nakuya Zoe 
Published: March 2024

BackgroundA substantial proportion of the world population is affected by malaria with 241 million malaria cases reported globally. Intermittent Preventive Treatment in pregnancy (IPTp) with Sulfadoxine-Pyrimethamine (SP) is an effective chemotherapy but its utilisation has not been optimised. Few studies focus on young mothers and their experiences regarding the optimal uptake of IPTp-SP.MethodsThe study design was cross-sectional with data derived from six focus group discussions with mothers aged 15–24 years who had a pregnancy and gave birth to a live baby within the last two years in Kisumu and Migori counties, Kenya. Inductive analysis was used to identify themes and patterns.ResultsYoung mothers were motivated to take IPTp-SP during pregnancy if they had prior knowledge about SP and its associated benefits and if they were knowledgeable about the consequences of malaria infection during pregnancy. Perceived side effects of SP, lack of awareness of SP as a malaria prevention therapy, lack of knowledge on the benefits of SP, dosage and frequency of uptake, poor communication by health providers towards young mothers, and inconsistent supply of SP at health facilities inhibited young mothers from attaining the recommended 3+ doses of IPTp-SP.ConclusionsThere is a need for health literacy programmes that focus on increasing knowledge of IPTp-SP dosage, timing and benefits for both the young pregnant mother and her foetus. Community engagement through dialogue with mentor mothers and male partners will be an important complementary approach in establishing a support system for young women for positive health outcomes including attaining the recommended 3+ doses of IPTp-SP.
Investigating peripheral blood monocyte and T-cell subsets as non-invasive biomarkers for asymptomatic hepatic steatosis: results from the Multi-Ethnic Study of Atherosclerosis
Authors: Niedecker Rhys W. , Delaney Joseph A. , Doyle Margaret F. , Sparks Andrew D. , Sitlani Colleen M. , Buzkova Petra , Zeb Irfan , Tracy Russell P. , Psaty Bruce M. , Budoff Matthew J. , Olson Nels C. 
Published: March 2024

BackgroundCirculating immune cells have gained interest as biomarkers of hepatic steatosis. Data on the relationships between immune cell subsets and early-stage steatosis in population-based cohorts are limited.MethodsThis study included 1,944 asymptomatic participants of the Multi-Ethnic Study of Atherosclerosis (MESA) with immune cell phenotyping and computed tomography measures of liver fat. Participants with heavy alcohol use were excluded. A liver-to-spleen ratio Hounsfield units (HU) &lt;1.0 and liver attenuation &lt;40 HU were used to diagnose liver fat presence and &gt;30% liver fat content, respectively. Logistic regression estimated cross-sectional associations of immune cell subsets with liver fat parameters adjusted for risk factors. We hypothesized that higher proportions of non-classical monocytes, Th1, Th17, and memory CD4+ T cells, and lower proportions of classical monocytes and naive CD4+ T cells, were associated with liver fat. Exploratory analyses evaluated additional immune cell phenotypes (n = 19).ResultsNone of the hypothesized cells were associated with presence of liver fat. Higher memory CD4+ T cells were associated with &gt;30% liver fat content, but this was not significant after correction for multiple hypothesis testing (odds ratio (OR): 1.31, 95% confidence interval (CI): 1.03, 1.66). In exploratory analyses unadjusted for multiple testing, higher proportions of CD8+CD57+ T cells were associated with liver fat presence (OR: 1.21, 95% CI: 1.02, 1.44) and &gt;30% liver fat content (OR: 1.34, 95% CI: 1.07, 1.69).ConclusionsHigher circulating memory CD4+ T cells may reflect liver fat severity. CD8+CD57+ cells were associated with liver fat presence and severity, but replication of findings is required.
Dysfunctional mitochondria, disrupted levels of reactive oxygen species, and autophagy in B cells from common variable immunodeficiency patients
Authors: Berman-Riu Maria , Cunill Vanesa , Clemente Antonio , López-Gómez Antonio , Pons Jaime , Ferrer Joana M. 
Published: March 2024

IntroductionCommon Variable Immunodeficiency (CVID) patients are characterized by hypogammaglobulinemia and poor response to vaccination due to deficient generation of memory and antibody-secreting B cells. B lymphocytes are essential for the development of humoral immune responses, and mitochondrial function, hreactive oxygen species (ROS) production and autophagy are crucial for determining B-cell fate. However, the role of those basic cell functions in the differentiation of human B cells remains poorly investigated.MethodsWe used flow cytometry to evaluate mitochondrial function, ROS production and autophagy processes in human naïve and memory B-cell subpopulations in unstimulated and stimulated PBMCs cultures. We aimed to determine whether any alterations in these processes could impact B-cell fate and contribute to the lack of B-cell differentiation observed in CVID patients.ResultsWe described that naïve CD19+CD27- and memory CD19+CD27+ B cells subpopulations from healthy controls differ in terms of their dependence on these processes for their homeostasis, and demonstrated that different stimuli exert a preferential cell type dependent effect. The evaluation of mitochondrial function, ROS production and autophagy in naïve and memory B cells from CVID patients disclosed subpopulation specific alterations. Dysfunctional mitochondria and autophagy were more prominent in unstimulated CVID CD19+CD27- and CD19+CD27+ B cells than in their healthy counterparts. Although naïve CD19+CD27- B cells from CVID patients had higher basal ROS levels than controls, their ROS increase after stimulation was lower, suggesting a disruption in ROS homeostasis. On the other hand, memory CD19+CD27+ B cells from CVID patients had both lower ROS basal levels and a diminished ROS production after stimulation with anti-B cell receptor (BCR) and IL-21.ConclusionThe failure in ROS cell signalling could impair CVID naïve B cell activation and differentiation to memory B cells. Decreased levels of ROS in CVID memory CD19+CD27+ B cells, which negatively correlate with their in vitro cell death and autophagy, could be detrimental and lead to their previously demonstrated premature death. The final consequence would be the failure to generate a functional B cell compartment in CVID patients.
Integrative single-cell analysis of LUAD: elucidating immune cell dynamics and prognostic modeling based on exhausted CD8+ T cells
Authors: Zhang Han , Zhang Pengpeng , Lin Xuefeng , Tan Lin , Wang Yuhang , Jia Xiaoteng , Wang Kai , Li Xin , Sun Daqiang 
Published: March 2024

BackgroundThe tumor microenvironment (TME) plays a pivotal role in the progression and metastasis of lung adenocarcinoma (LUAD). However, the detailed characteristics of LUAD and its associated microenvironment are yet to be extensively explored. This study aims to delineate a comprehensive profile of the immune cells within the LUAD microenvironment, including CD8+ T cells, CD4+ T cells, and myeloid cells. Subsequently, based on marker genes of exhausted CD8+ T cells, we aim to establish a prognostic model for LUAD.MethodUtilizing the Seurat and Scanpy packages, we successfully constructed an immune microenvironment atlas for LUAD. The Monocle3 and PAGA algorithms were employed for pseudotime analysis, pySCENIC for transcription factor analysis, and CellChat for analyzing intercellular communication. Following this, a prognostic model for LUAD was developed, based on the marker genes of exhausted CD8+ T cells, enabling effective risk stratification in LUAD patients. Our study included a thorough analysis to identify differences in TME, mutation landscape, and enrichment across varying risk groups. Moreover, by integrating risk scores with clinical features, we developed a new nomogram. The expression of model genes was validated via RT-PCR, and a series of cellular experiments were conducted, elucidating the potential oncogenic mechanisms of GALNT2.ResultsOur study developed a single-cell atlas for LUAD from scRNA-seq data of 19 patients, examining crucial immune cells in LUAD’s microenvironment. We underscored pDCs’ role in antigen processing and established a Cox regression model based on CD8_Tex-LAYN genes for risk assessment. Additionally, we contrasted prognosis and tumor environments across risk groups, constructed a new nomogram integrating clinical features, validated the expression of model genes via RT-PCR, and confirmed GALNT2’s function in LUAD through cellular experiments, thereby enhancing our understanding and approach to LUAD treatment.ConclusionThe creation of a LUAD single-cell atlas in our study offered new insights into its tumor microenvironment and immune cell interactions, highlighting the importance of key genes associated with exhausted CD8+ T cells. These discoveries have enabled the development of an effective prognostic model for LUAD and identified GALNT2 as a potential therapeutic target, significantly contributing to the improvement of LUAD diagnosis and treatment strategies.