bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANGIOTENSIN HYPERTENSION
Authors: Blaine Edward H. , Cunningham J Thomas , Hasser Eileen M. , Dale William E. , Li Qian , Sullivan Margaret 
Published: June 2007

SUMMARY1. One of the most important issues in the field of hypertension research centres on the therapeutic use of inhibitors of the renin‐angiotensin system (RAS). Inhibitors of the RAS have potent anti‐hypertensive effects, even in experimental models of hypertension and in human essential hypertension, where the activity of the peripheral RAS is low or normal.2. It is suggested here that determining the mechanisms by which activation of the peripheral RAS produces hypertension will help us determine the anti‐hypertensive effects of these inhibitors in low/normal renin‐angiotensin hypertension.3. Three hypotheses describing the hypertensive effects of angiotensin are discussed. The first hypothesis involves the direct vasoconstrictor effects of angiotensin. The second hypothesis suggests that chronic angiotensin produces hypertension by increasing Na+reabsorption leading to volume expansion and hypertension. The final hypothesis suggests that, in angiotensin‐induced hypertension, the increased Na+reabsorption is not associated with volume expansion but, rather, is associated with an increase in vascular tone resulting from an interaction between angiotensin and the nervous system.4. It is also hypothesized that the interaction between angiotensin and the nervous system produces a differential activation of sympathetic outflow that spares the kidney.
DIFFICULTIES IN DETECTING PARATHYROID HYPERTENSIVE FACTOR IN THE RAT
Authors: Mangos George J , Brown Mark A , Whitworth Judith A 
Published: June 2007

SUMMARY1. Parathyroid hypertensive factor (PHF) has been implicated in the pathogenesis of several forms of hypertension. We aimed to establish a PHF bioassay using spontaneously hypertensive rat (SHR) plasma.2. Spontaneously hypertensive rats were confirmed hypertensive and their plasma was dialysed and injected into anaesthetized normotensive rats.3. Haemodynamic responses to injected SHR plasma were uninterpretable during pentobarbital anaesthesia due to blood pressure (BP) fluctuation. Under halothane anaesthesia, BP was stable but did not rise following SHR plasma injection. Using filtered, undialysed plasma and a different brand of pentobarbital, no consistent BP response was observed following SHR plasma injection.4. We were unable to detect PHF in the SHR.
MYOCARDIAL DYSFUNCTION IN SEPSIS
Authors: Piper Richard D 
Published: June 2007

SUMMARY1. Sepsis is the leading reversible cause of death in patients requiring modern intensive care services.2. In this group of patients, death usually results from progressive multiple organ failure, rather than overwhelming primary infection.3. The pathophysiology of sepsis‐induced remote organ dysfunction is incompletely understood, although it is believed to result from a systemic inflammatory process that causes tissue injury in the absence of septic shock.4. As septic shock is the most common early manifestation of severe sepsis, an understanding of mechanisms of myocardial dysfunction is of clinical relevance. In the present review, we will discuss mechanisms of remote organ failure in sepsis, focusing in particular on the pathogenesis of myocardial dysfunction.
Bioenergetic and oxidative effects of free 3‐nitrotyrosine in culture: selective vulnerability of dopaminergic neurons and increased sensitivity of non‐dopaminergic neurons to dopamine oxidation
Authors: Ma Thong C. , Mihm Michael J. , Bauer John Anthony , Hoyt Kari R. 
Published: June 2007

AbstractProtein bound and free 3‐nitrotyrosine (3NT) levels are elevated in neurodegenerative diseases and have been used as evidence for peroxynitrite generation. Intrastriatal injection of free 3NT causes dopaminergic neuron injury and represents a new mouse model of Parkinson’s disease (PD). We are investigating the nature of free 3NT neurotoxicity. In primary ventral midbrain cultures, free 3NT damaged dopaminergic neurons, while adjacent non‐dopaminergic neurons were unaffected. Combined treatment with free 3NT and subtoxic amounts of dopamine caused extensive death of non‐dopaminergic forebrain neurons in culture. Free 3NT alone directly inhibited mitochondrial complex I, decreased ATP, sensitized neurons to mitochondrial depolarization, and increased superoxide production. Subtoxic concentrations of rotenone (instead of free 3NT) caused similar results. Additionally, free 3NT and dopamine combined increased extraneuronal hydrogen peroxide and decreased intraneuronal glutathione levels more than dopamine alone. Oxidative and bioenergetic processes have been proposed to contribute to neurodegeneration in PD. As free 3NT is a compound that is increased in PD, damages dopamine neurons in vivo and in vitro and has detrimental effects on neuronal bioenergetics, it is possible that free 3NT is an endogenous contributing factor to neuronal loss, in addition to being a marker of oxidative and nitrative processes.
Alcohol Consumption and Alcoholic Liver Disease: Evidence of a Threshold Level of Effects of Ethanol
Authors: Savolainen V. T. , Liesto K. , Männikkö A. , Penttilä A. , Karhunen P. J. 
Published: June 2007

The effects of long‐term moderate or “social” alcohol consumption (10‐80 g daily intake) on the incidence of features of alcoholic liver disease (ALD) were delineated in a consecutive autopsy series of 210 males. The subjects' daily intake, as well as duration of alcohol consumption, was determined by an interview with the spouse or a close acquaintance and compared with semiquantitative histological scores for stage of ALD.No significant increase in the incidence of features of ALD could be related to all‐year daily intake of ethanol below 40 g (40 g equals 1.1 liter of beer, 0.44 liter of wine, and 0.11 liter of spirits). However, daily intake between 40–80 g increased relative liver weight on average 3.1 g/kg of body weight (p < 0.02), the frequency of fatty liver from 11.7 to 47.2% [relative risk (RR) = 4.4], and the frequency of mainly slight alcoholic hepatitis up to 16.7% (RR = 7.5). The incidence of both bridging fibrosis and liver cirrhosis increased significantly (RR = 8.8) only when daily intake exceeded 80 g. Amounts of ethanol exceeding 80 g did not relate to further increases in incidence of bridging fibrosis or liver cirrhosis.These findings suggest that, in males, daily ingestion of ethanol below 40 g for a period of 25 years does not increase the risk of alcohol‐related liver disease. In contrast, similar duration of daily intake between 40 and 80 g (mean 61.6 g) increased the risk of all but fibrotic liver lesions of ALD significantly and may thus represent a potential threshold level that significantly increases the risk of alcohol‐related liver damage. Moreover, our results may suggest that, on an individual level, the risk function for liver fibrosis may not be directly dose‐related, but rather, when a permissive threshold level is attained, further consumption is of no or little importance to the progression of ALD.
Upper arm circumference is associated with race performance in ultra-endurance runners
Authors: Knechtle B , Knechtle P , Schulze I , Kohler G 
Published: June 2007

Objective:To investigate the association of anthropometric parameters to race performance in ultra-endurance runners in a multistage ultra-endurance run.Design:Descriptive field study.Setting:The Deutschlandlauf 2006 race in Germany, where athletes had to run 1200 km within 17 consecutive days. There were no interventions.Subjects:In total, there were 19 male Caucasian ultra-endurancerunners (mean (SD) 46.2 (9.6) years, 71.8 (5.2) kg, 179 (6) cm, BMI 22.5 (1.9) kg/m2).Main outcome measurements:Determination of body mass, body height, length of lower limbs, skin-fold thicknesses, circumference of limbs, body mass index (BMI), percentage skeletal muscle mass (%SM), and percentage body fat (%BF) in 19 successful finishers in order to correlate anthropometric parameters with running performance.Results:A significant association of upper arm circumference with the total running time was found (p<0.05, r2 = 0.26). No significant association was found with the directly measured anthropometric properties body height, body mass, average skin-fold thickness and the circumference of thigh and calf (p>0.05). Furthermore, no significant association was observed between the running time and the calculated parameters BMI, %BF, and %SM (p>0.05).Conclusions:In an ultra-endurance run over 1200 km within 17 consecutive days, circumference of the upper arm was the only factor associated with performance in well-experienced ultra-endurance runners. Body mass, BMI, body height, length of limbs, skin-fold thicknesses, circumference of limbs and the calculated percentage body composition of skeletal muscle mass and body fat showed no association with running performance.
Indo-Pacific Sea Surface Temperature Perturbations Associated with Intraseasonal Oscillations of Tropical Convection
Authors: Duvel Jean Philippe , Vialard Jérôme 
Published: June 2007

Abstract
Since the ISV of the convection is an intermittent phenomenon, the local mode analysis (LMA) technique is used to detect only the ensemble of intraseasonal events that are well organized at large scale. The LMA technique is further developed in this paper in order to perform multivariate analysis given patterns of SST and surface wind perturbations associated specifically with these intraseasonal events. During boreal winter, the basin-scale eastward propagation of the convective perturbation is present only over the Indian Ocean Basin. The intraseasonal SST response to convective perturbations is large and recurrent over thin mixed layer regions located north of Australia and in the Indian Ocean between 5° and 10°S. By contrast, there is little SST response in the western Pacific basin and no clear eastward propagation of the convective perturbation. During boreal summer, the SST response is large over regions with thin mixed layers located north of the Bay of Bengal, in the Arabian Sea, and in the China Sea. The northeastward propagation of the convective perturbation over the Bay of Bengal is associated with a standing oscillation of the SST and the surface wind between the equator and the northern part of the bay. In fact, many intraseasonal events mostly concern a single basin, suggesting that the interbasin organization is not a necessary condition for the existence of coupled intraseasonal perturbations of the convection.
The perturbation of the surface wind tends to be larger to the west of the large-scale convective perturbation (like for a Gill-type dynamical response). For eastward propagating perturbations, the cooling due to the reinforcement of the wind (i.e., surface turbulent heat flux) thus generally lags the radiative cooling due to the reduction of the surface solar flux by the convective cloudiness. This large-scale Gill-type response of the surface wind also cools the surface to the west of the basin (northwest Arabian Sea and northwest Pacific Ocean), even if the convection is locally weak. An intriguing result is a frequently occurring small delay between the maximum surface wind and the minimum SST. Different explanations are invoked, like a rapid surface cooling due to the vanishing of an ocean warm layer (diurnal surface warming due to solar radiation in low wind conditions) as soon as the wind increases.
Possible Impact of the Indian Ocean SST on the Northern Hemisphere Circulation during El Niño*
Authors: Annamalai H. , Okajima H. , Watanabe M. 
Published: June 2007

Abstract
Two atmospheric general circulation models (AGCMs), differing in numerics and physical parameterizations, are employed to test the hypothesis that El Niño–induced sea surface temperature (SST) anomalies in the tropical Indian Ocean impact considerably the Northern Hemisphere extratropical circulation anomalies during boreal winter [January–March +1 (JFM +1)] of El Niño years. The hypothesis grew out of recent findings that ocean dynamics influence SST variations over the southwest Indian Ocean (SWIO), and these in turn impact local precipitation. A set of ensemble simulations with the AGCMs was carried out to assess the combined and individual effects of tropical Pacific and Indian Ocean SST anomalies on the extratropical circulation. To elucidate the dynamics responsible for the teleconnection, solutions were sought from a linear version of one of the AGCMs.
Both AGCMs demonstrate that the observed precipitation anomalies over the SWIO are determined by local SST anomalies. Analysis of the circulation response shows that over the Pacific–North American (PNA) region, the 500-hPa height anomalies, forced by Indian Ocean SST anomalies, oppose and destructively interfere with those forced by tropical Pacific SST anomalies. The model results validated with reanalysis data show that compared to the runs where only the tropical Pacific SST anomalies are specified, the root-mean-square error of the height anomalies over the PNA region is significantly reduced in runs in which the SST anomalies in the Indian Ocean are prescribed in addition to those in the tropical Pacific. Among the ensemble members, both precipitation anomalies over the SWIO and the 500-hPa height over the PNA region show high potential predictability. The solutions from the linear model indicate that the Rossby wave packets involved in setting up the teleconnection between the SWIO and the PNA region have a propagation path that is quite different from the classical El Niño–PNA linkage.
The results of idealized experiments indicate that the Northern Hemisphere extratropical response to Indian Ocean SST anomalies is significant and the effect of this response needs to be considered in understanding the PNA pattern during El Niño years. The results presented herein suggest that the tropical Indian Ocean plays an active role in climate variability and that accurate observation of SST there is of urgent need.
Explorations of the Annual Mean Heat Budget of the Tropical Indian Ocean. Part I: Studies with an Idealized Model
Authors: Godfrey J. Stuart , Hu Rui-Jin , Schiller Andreas , Fiedler R. 
Published: June 2007

Abstract
Annual mean net heat fluxes from ocean general circulation models (OGCMs) are systematically too low in the tropical Indian Ocean, compared to observations. In the models, only some of the geostrophic inflow replacing southward Ekman outflow is colder than the minimum sea surface temperature (MINSST). Observed heat fluxes imply that much more inflow is colder than MINSST. Since inflow below MINSST can only join the surface Ekman transport after diathermal warming, the OGCMs must underestimate diathermal effects.
A crude analog of the annual mean Indian Ocean heat budget was generated, using a rectangular box model with a deep “Indo–Pacific” gap at 7°–10°S in its eastern side. Wind stress was zonal and proportional to the Coriolis parameter, so Ekman transport was spatially constant and equaled Sverdrup transport. For three experiments, zonally integrated Ekman transport was steady and southward at 10 Sv (Sv ≡ 106 m3 s−1). In steady state, a 10 Sv “Indonesian Throughflow” fed a northward western boundary current of 10 Sv, which turned eastward along the northern boundary at 10°N to feed the southward Ekman transport. Most diathermal mixing occurred within an intense eddy in the northwest corner. Some of the geostrophic inflow was at temperatures colder than MINSST (found at the northeast corner of the eddy); it must warm to MINSST via diathermal mixing. Northern boundary upwelling exceeded the 10-Sv Ekman transport. The excess warms as it recirculates around the eddy, apparently supplying the heat to warm inflow below MINSST. In an experiment using the “flux-corrected transport” (FCT) scheme, diathermal mixing occurred in the strongly sheared currents around the eddy. However the Richardson number never became low enough to drive strong diathermal mixing, perhaps because (like that of other published models) the present model’s vertical resolution was too coarse. In three experiments, the dominant mixing was caused by horizontal diffusion, spurious convective overturn, and numerical mixing invoked by the FCT scheme, respectively. All three mixing mechanisms are physically suspect; such model problems (if widespread) must be resolved before the mismatch between observed and modeled heat fluxes can be addressed. However, the fact that the density profile at the western boundary must be hydrostatically stable places a lower limit on the area-integrated heat fluxes. Results from the three main experiments—and from many published OGCMs—are quite close to this lower limit.
Mechanisms for the Interannual Variability in the Tropical Indian Ocean. Part II: Regional Processes
Authors: Huang Bohua , Shukla J. 
Published: June 2007

Abstract
To understand the mechanisms of the interannual variability in the tropical Indian Ocean, two long-term simulations are conducted using a coupled ocean–atmosphere GCM—one with active air–sea coupling over the global ocean and the other with regional coupling restricted within the Indian Ocean to the north of 30°S while the climatological monthly sea surface temperatures (SSTs) are prescribed in the uncoupled oceans to drive the atmospheric circulation. The major spatial patterns of the observed upper-ocean heat content and SST anomalies can be reproduced realistically by both simulations, suggesting that they are determined by intrinsic coupled processes within the Indian Ocean.
In both simulations, the interannual variability in the Indian Ocean is dominated by a tropical mode and a subtropical mode. The tropical mode is characterized by a coupled feedback among thermocline depth, zonal SST gradient, and wind anomalies over the equatorial and southern tropical Indian Ocean, which is strongest in boreal fall and winter. The tropical mode simulated by the global coupled model reproduces the main observational features, including a seasonal connection to the model El Niño–Southern Oscillation (ENSO). The ENSO influence, however, is weaker than that in a set of ensemble simulations described in Part I of this study, where the observed SST anomalies for 1950–98 are prescribed outside the Indian Ocean. Combining with the results from Part I of this study, it is concluded that ENSO can modulate the temporal variability of the tropical mode through atmospheric teleconnection. Its influence depends on the ENSO strength and duration. The stronger and more persistent El Niño events in the observations extend the life span of the anomalous events in the tropical Indian Ocean significantly. In the regional coupled simulation, the tropical mode is still active, but its dominant period is shifted away from that of ENSO. In the absence of ENSO forcing, the tropical mode is mainly stimulated by an anomalous atmospheric direct thermal cell forced by the fluctuations of the northwestern Pacific monsoon.
The subtropical mode is characterized by an east–west dipole pattern of the SST anomalies in the southern subtropical Indian Ocean, which is strongest in austral fall. The SST anomalies are initially forced by surface heat flux anomalies caused by the anomalous southeast trade wind in the subtropical ocean during austral summer. The trade wind anomalies are in turn associated with extratropical variations from the southern annular mode. A thermodynamic air–sea feedback strengthens these subtropical anomalies quickly in austral fall and extends their remnants into the tropical ocean in austral winter. In the simulations, this subtropical variability is independent of ENSO.
A model-based optimization framework for the inference of regulatory interactions using time-course DNA microarray expression data
Authors: Thomas Reuben , Paredes Carlos J , Mehrotra Sanjay , Hatzimanikatis Vassily , Papoutsakis Eleftherios T 
Published: June 2007

Abstract

Background
Proteins are the primary regulatory agents of transcription even though mRNA expression data alone, from systems like DNA microarrays, are widely used. In addition, the regulation process in genetic systems is inherently non-linear in nature, and most studies employ a time-course analysis of mRNA expression. These considerations should be taken into account in the development of methods for the inference of regulatory interactions in genetic networks.

Results
We use an S-system based model for the transcription and translation process. We propose an optimization-based regulatory network inference approach that uses time-varying data from DNA microarray analysis. Currently, this seems to be the only model-based method that can be used for the analysis of time-course "relative" expressions (expression ratios). We perform an analysis of the dynamic behavior of the system when the number of experimental samples available is varied, when there are different levels of noise in the data and when there are genes that are not considered by the experimenter. Our studies show that the principal factor affecting the ability of a method to infer interactions correctly is the similarity in the time profiles of some or all the genes. The less similar the profiles are to each other the easier it is to infer the interactions. We propose a heuristic method for resolving networks and show that it displays reasonable performance on a synthetic network. Finally, we validate our approach using real experimental data for a chosen subset of genes involved in the sporulation cascade of Bacillus anthracis. We show that the method captures most of the important known interactions between the chosen genes.

Conclusion
The performance of any inference method for regulatory interactions between genes depends on the noise in the data, the existence of unknown genes affecting the network genes, and the similarity in the time profiles of some or all genes. Though subject to these issues, the inference method proposed in this paper would be useful because of its ability to infer important interactions, the fact that it can be used with time-course DNA microarray data and because it is based on a non-linear model of the process that explicitly accounts for the regulatory role of proteins.
Requirement of histone deacetylase activity for the expression of critical photoreceptor genes
Authors: Chen Bo , Cepko Constance L 
Published: June 2007

Abstract
Background
Histone deacetylases (HDACs) play a major role in the regulation of gene transcription, often leading to transcriptional repression, as well as other effects following deacetylation of non-histone proteins.

Results
To investigate the role of HDACs in the developing mammalian retina, a general inhibitor of HDACs, trichostatin-A (TSA), was used to treat newborn murine retinae in explant cultures. Inhibition of HDAC activity resulted in a reduction in RNA levels for genes that regulate retinal development, as well as cell cycle regulators. Several of the genes encode transcription factors essential for rod photoreceptor development, Otx2, Nrl, and Crx. Using luciferase reporter assays, the promoter activity of both Nrl and Crx was found to be compromised by HDAC inhibition. Furthermore, downregulation of gene expression by HDAC inhibition didn't require de novo protein synthesis, and was associated with hyperacetylation of histones and non-histone proteins. Finally, HDAC inhibition in retinal explant cultures resulted in increased cell death, reduction in proliferation, a complete loss of rod photoreceptors and Müller glial cells, and an increase in bipolar cells.

Conclusion
HDAC activity is required for the expression of critical pro-rod transcription factors and the development of rod photoreceptor cells.