Pacific Rim Forestry / Silviculture and Forest Ecology
Authors: Negi Vishal Singh , Pal Archana , Singh Ratnesh , Borthakur Dulal , Pala Archana , Negi Vishal Singh , Borthakur Dulal , Rushanaedy Isabel , Jones Tyler , Dudley Nicklos , Borthakur Dulal , Yafuso Jannai , Negi Vishal Singh , Borthakur Dulal , Friday Kathleen , Sanney Jacob , Nithan Maxson , Jonathan Rickson , Waguk Erick , Friday Kathleen , Michael Uelbil Puanani , Sadang Omekrael , Friday Kathleen , Eperiam Eugene , Marquez Wendolin Roseo , Friday Kathleen , Maugalei Fa'afa'i Tony , Sue John Ah , Friday Kathleen , Jones Tyler , Dudley Nicklos , Strom Brian , Smith Sheri , Fischer Joseph , Jones Tyler , Dudley Nicklos , Borthakur Dulal , Yeh Aileen , Sniezko Richard , Cannon Philip , James Robert , Schenck Susan , Raboin Erin , Johnson M. Tracy , Denslow Julie , Liu Zhanfeng , Gurr Neil , Schmaedick Mark , Whistler Arthur , Fischer Lisa , Jones Tyler , Dudley Nicklos , James Robert , Borthakur Dulal , Cannon Philip , Sniezko Richard , Schenck Susan , Kolka Randy , Hanson Paul , Norby Rich , Palik Brian , Wullschleger Stan , Garten Charles , Sebestyen Steve , Thornton Peter , Bradford John , Mulhollend Pat , Todd Don , Iversen Colleen , Warren Jeff , Peterson Kristin , Shiraishi Ayami , Uchida Janice , Tacconelli Michelle , Loewenstein Edward , Bell Conor , Kettler Kenneth , Premer Michael , Oikarinen Kara , Dunnack Eric , Pickens James , Burkowski Marilynn , Gazal Rico , Dugan Sean , Oswald Brian , Balice Randy , Unger Daniel , Kreye Melissa , Stein Taylor , Ober Holly , Kobziar Leda , Bohn Kimberly , Carter Douglas , Peterson Nancy , Doruska Paul , Vacchiano Giorgio , Berretti Roberta , Motta Renzo , Michaels Christy , Oswald Brian , Williams Hans , Farrish Kenneth , Schuler Jamie , Litton Creighton , Giardina Christian , Crow Susan , Fortini Lucas , Schultz Emma , Seymour Robert , Weiskittel Aaron , Droske Christine , Morgan Penelope , Davis Anthony S. , Robichaud Peter R. , Lewis Sarah , Hudak Andrew T. , Lentile Leigh B. , Francis Paul , Orr Blair , Long Michael , Litton Creighton , Giardina Christian , Sparks Jed , Macy Thomas , Goebel Charles , Hix David , Miller David , Rudnicki Mark , Hiscox April , Su Hong Bing , Kolb Thomas , Previant Wilfred , Nagel Linda , Webster Christopher , Fuller Angela , Harrison Daniel , DeRose Justin , Shaw John , Long Jim , Bentz Barbara , Holzmueller Eric , Gibson David , Suchecki Paul , Stuhlinger Chris , Silver Emily , D'Amato Anthony , Fraver Shawn , Palik Brian , Strommer Shane , Teets Andrew , Houghton Craig , Gundlach Andrew , Fritz Brian , Brantley Elizabeth , Stuhlinger Chris , Cunningham Kyle , Pelkki Matthew , Caldwell Benjamin , Kusbach Antonin , Shaw John , Long Jim , Eiche Nik , Strom Brian , Smith Sheri , Jones Dryw , Lightle Nicole , Heckathorn Scott , Morton Nathaniel , Weigle Brad , McGown Kathryn , Miller Joshua , Stewart Jane , Ross-Davis Amy , Davis Anthony S. , Chhin Sophan , Mbow Cheikh , Sambou Bienvenu , Skole David , Gottschalk Kurt , Miller Gary , Brose Patrick , Tolley Grant , Battaglia Mike , Jain Theresa , Han Han-Sup , Graham Russell , Keyes Christopher , Fried Jeremy 
Published: April 2024

Abstract
Leucaena leucocephala (leucaena) is an important agroforestry tree of tropics and subtropics due to its protein rich foliages, ability to grow on poor soils, and high level of tolerance to various stresses including drought and diseases. We hypothesized that leucaena have a unique set of genes which confer tolerance to various abiotic and biotic stresses and these genes are either absent or not expressed in other legumes that are susceptible to these stress conditions. Interspecies suppression subtractive hybridization (iSSH) was performed to identify some unique genes from leucaena using cDNAs from leucaena and Acacia confusa (acacia) as the tester and driver, respectively. We identified 100 leucaena genes showing homology to various plant genes with known functions, of which 23 genes had homology to various stress-related proteins including chitinase, PR-10 protein, wound-stress protein, type-2 metallothionein, thaumatin-like protein, disease resistance response protein, cysteine proteinase, and kunitz trypsin inhibitor. We also identified 50 leucaena genes which either had homology to hypothetical proteins (HyPs) or had no homology at all. Putative functions of 15 HyPs were predicted using conserved domain and PSI-BLAST analyses. Linear motif analyses identified 17 HyPs with MAPK recognition motif, which is involved in signaling pathways, including stress responses. This study identified 23 known and some putative unique stress-related genes from leucaena which may serve as a useful source of stress-tolerance genes for tree and crop improvement in the future.