bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel Bach2 transcripts and protein isoforms through tagging analysis of retroviral integrations in B-cell lymphomas
Authors: Liu Jinghua , Sørensen Annette Balle , Wang Bruce , Wabl Matthias , Nielsen Anders Lade , Pedersen Finn Skou 
Published: January 2009

Abstract

Background
The Bach2 gene functions as a transcriptional repressor in B-cells, showing high expression level only before the plasma cell stage. Several lines of evidence indicate that Bach2 is a B-cell specific tumor suppressor. We here address patterns of insertional mutagenesis and expression of Bach2 is a murine retroviral model of B-cell lymphoma induction.

Results
We report that the Bach2 gene is a target of proviral integrations in B-cell lymphomas induced by murine leukemia virus. An alternative Bach2 promoter was identified within intron 2 and this promoter was activated in one of the tumors harboring proviral integration. The alternative promoter was active in both normal and tumor tissue and the tissue specificity of the two Bach2 promoters was similar. Three different alternatively used Bach2 terminal exons were identified to be located in intron 4. The inclusion of these exons resulted in the generation of Bach2 mRNA with open reading frames lacking the bZIP DNA binding domain present in the normal Bach2 protein, but retaining a partial BTB protein dimerization domain. Such Bach2 protein was excluded from the cell nucleus.

Conclusion
We have identified an alternative promoter and new protein isoforms of Bach2. Our data imply that activation of an alternative promoter by proviral integration serves as a possible mechanism of up-regulation of the Bach2 gene with a potential role in B-cell lymphomagenesis. The finding of novel Bach2 transcripts and protein isoforms will facilitate a better insight into the normal and pathophysiological regulation of the Bach2 gene.
Evidence for separate translocation pathways in determining cadmium accumulation in grain and aerial plant parts in rice
Authors: Kashiwagi Takayuki , Shindoh Kumiko , Hirotsu Naoki , Ishimaru Ken 
Published: January 2009

Abstract

Background
Cadmium (Cd) translocation and accumulation in the grain and aerial plant parts of rice (Oryza sativa L.) is an important aspect of food safety and phytoextraction in areas with contaminated soil. Because control of Cd translocation and accumulation is likely to be determined by the plants genetics, the Cd contents of grain and the aerial parts of rice may be manipulated to improve food safety and for phytoextraction ability. This study studied Cd translocation and accumulation and their genetic control in aerial parts of rice to provide a starting point for improving food safety and phytoextraction in Cd-contaminated soils.

Results
In the japonica rice cultivar "Nipponbare", Cd accumulated in leaves and culms until heading, and in culms and ears after heading. Two quantitative trait loci (QTLs) from indica cv. "Kasalath", qcd4-1 and qcd4-2, affect Cd concentrations in upper plant parts just before heading. Three near-isogenic lines (NILs) with qcd4-1 and qcd4-2 were selected from the "Nipponbare" background, and were analyzed for the effects of each QTL, and for interactions between the two QTLs. From the results compared between "Nipponbare" and each NIL, neither QTL influenced total Cd accumulation in aerial parts at 5 days after heading, but the interaction between two QTLs increased Cd accumulation. At 35 days after heading, qcd4-2 had increased Cd accumulation in the aerial plant parts and decreased translocation from leaves other than flag leaf, but interaction between the two QTLs increased translocation from leaves. NILqcd4-1,2 accumulated higher concentrations of Cd in brown rice than "Nipponbare".

Conclusion
Three types of Cd translocation and accumulation patterns demonstrated by NILs suggested that the accumulation of Cd in leaves and culms before heading, and translocation from them after heading are responsible for Cd accumulation in grain. Cd translocation from roots to culms and ears after heading may direct Cd to the aerial organs without influencing brown rice accumulation.
n3 and n6 polyunsaturated fatty acids differentially modulate prostaglandin E secretion but not markers of lipogenesis in adipocytes
Authors: Wortman Patrick , Miyazaki Yuko , Kalupahana Nishan S , Kim Suyeon , Hansen-Petrik Melissa , Saxton Arnold M , Claycombe Kate J , Voy Brynn H , Whelan Jay , Moustaid-Moussa Naima 
Published: January 2009

Abstract
A dramatic rise in the incidence of obesity in the U.S. has accelerated the search for interventions that may impact this epidemic. One recently recognized target for such intervention is adipose tissue, which secretes a variety of bioactive substances including prostaglandins. Prostaglandin E2 (PGE2) has been shown to decrease lipolysis in adipocytes, but limited studies have explored alternative mechanisms by which PGE2 might impact obesity, such as adipogenesis or lipogenesis. Studies conducted on Apc
Min/+ mice indicated that selective inhibition of the cyclooxygenase (COX)-2 enzyme led to significant reductions in fatty acid synthase (FAS) activity in adipose tissue suggesting lipogenic effects of PGE2. To further investigate whether these lipid mediators directly regulate lipogenesis, we used 3T3-L1 adipocytes to determine the impact of eicosapentaenoic acid (EPA) and celecoxib on PGE2 formation and FAS used as a lipogenic marker. Both arachidonic acid (AA) and EPA dose-dependently increased PGE secretion from adipocytes. AA was expectedly more potent and exhibiting at 150 uM dose a 5-fold increase in PGE2 secretion over EPA. Despite higher secretion of PGE by EPA and AA compared to control, neither PUFA significantly altered FAS activity. By contrast both AA and EPA significantly decreased FAS mRNA levels. Addition of celecoxib, a selective COX-2 inhibitor, significantly decreased PGE2 secretion (p < 0.05) versus control, and also significantly decreased FAS activity (p < 0.05). Unexpectedly, the combination of exogenous PGE2 and celecoxib further decreased the FAS activity compared to PGE2 alone or untreated controls. In conclusion, EPA-mediated inhibition of AA metabolism did not significantly alter FAS activity while both AA and EPA significantly decreased FAS mRNA expression. COX-2 inhibition significantly decreased PGE2 production resulting in a decrease in FAS activity and expression that was not reversed with the addition of exogenous PGE2, suggesting an additional mechanism that is independent of COX-2.
Effect of ablated hippocampal neurogenesis on the formation and extinction of contextual fear memory
Authors: Ko Hyoung-Gon , Jang Deok-Jin , Son Junehee , Kwak Chuljung , Choi Jun-Hyeok , Ji Young-Hoon , Lee Yun-Sil , Son Hyeon , Kaang Bong-Kiun 
Published: January 2009

Abstract
Newborn neurons in the subgranular zone (SGZ) of the hippocampus incorporate into the dentate gyrus and mature. Numerous studies have focused on hippocampal neurogenesis because of its importance in learning and memory. However, it is largely unknown whether hippocampal neurogenesis is involved in memory extinction per se. Here, we sought to examine the possibility that hippocampal neurogenesis may play a critical role in the formation and extinction of hippocampus-dependent contextual fear memory. By methylazoxymethanol acetate (MAM) or gamma-ray irradiation, hippocampal neurogenesis was impaired in adult mice. Under our experimental conditions, only a severe impairment of hippocampal neurogenesis inhibited the formation of contextual fear memory. However, the extinction of contextual fear memory was not affected. These results suggest that although adult newborn neurons contribute to contextual fear memory, they may not be involved in the extinction or erasure of hippocampus-dependent contextual fear memory.
Recent advances of novel targeted therapy in non-small cell lung cancer
Authors: Katzel Jed A , Fanucchi Michael P , Li Zujun 
Published: January 2009

Abstract
Lung cancer is the leading cause of cancer deaths world-wide. Recent advances in cancer biology have led to the identification of new targets in neoplastic cells and the development of novel targeted therapies. At this time, two targeted agents are approved by the FDA in advanced non-small cell lung cancer (NSCLC): the epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) erlotinib, and the anitangiogenic bevacizumab. A third agent, cetuximab, which was recently shown to enhance survival when used with cisplatin and vinorelbine as first line therapy for advanced NSCLC, will likely be approved by regulatory agencies. With more than 500 molecularly targeted agents under development, the prospects of identifying novel therapies that benefit individual patients with lung cancer are bright.
Prognostic value of metastin expression in human pancreatic cancer
Authors: Nagai Kazuyuki , Doi Ryuichiro , Katagiri Fumihiko , Ito Tatsuo , Kida Atsushi , Koizumi Masayuki , Masui Toshihiko , Kawaguchi Yoshiya , Tomita Kenji , Oishi Shinya , Fujii Nobutaka , Uemoto Shinji 
Published: January 2009

Abstract

Background

KiSS-1 was identified as a metastasis-suppressing gene in melanoma cells. The KiSS-1 gene product (metastin) was isolated from human placenta as the ligand of GPR54, a G-protein-coupled receptor. The role of metastin and GPR54 in tumor progression is not fully understood.

Methods
We investigated the clinical significance of metastin and GPR54 expression in pancreatic cancer. We evaluated immunohistochemical expression of metastin and GPR54 in pancreatic ductal adenocarcinoma tissues obtained from 53 consecutive patients who underwent resection between July 2003 and May 2007 at Kyoto University Hospital. In 23 consecutive patients, the plasma metastin level was measured before surgery by enzyme immunoassay.

Results
Strong immunohistochemical expression of metastin was detected in 13 tumors (24.5%), while strong expression of GPR54 was detected in 30 tumors (56.6%). Tumors that were negative for both metastin and GPR54 expression were significantly larger than tumors that were positive for either metastin or GPR54 (p = 0.047). Recurrence was less frequent in patients who had metastin-positive tumors compared with those who had metastin-negative tumors (38.5% versus 70.0%, p = 0.04). Strong expression of metastin and GPR54 was significantly correlated with longer survival (p = 0.02). Metastin expression by pancreatic cancer was an independent prognostic factor for longer survival (hazard ratio, 2.1; 95% confidence interval, 1.1–4.7; p = 0.03), and the patients with a high plasma metastin level (n = 6) did not die after surgical resection.

Conclusion
Strong expression of metastin and GPR54 by pancreatic cancer is associated with longer survival. Metastin expression is an independent prognostic factor for the survival of pancreatic cancer patients. The plasma metastin level could become a noninvasive prognostic factor for the assessment of pancreatic cancer.
Prolonged Treatments With Antiresorptive Agents and PTH Have Different Effects on Bone Strength and the Degree of Mineralization in Old Estrogen-Deficient Osteoporotic Rats
Authors: Cheng Zhiqiang , Yao Wei , Zimmermann Elizabeth A , Busse Cheryl , Ritchie Robert O , Lane Nancy E 
Published: January 2009

Abstract
Current approved medical treatments for osteoporosis reduce fracture risk to a greater degree than predicted from change in BMD in women with postmenopausal osteoporosis. We hypothesize that bone active agents improve bone strength in osteoporotic bone by altering different material properties of the bone. Eighteen-month-old female Fischer rats were ovariectomized (OVX) or sham-operated and left untreated for 60 days to induce osteopenia before they were treated with single doses of either risedronate (500 μg/kg, IV), zoledronic acid (100 μg/kg, IV), raloxifene (2 mg/kg, PO, three times per week), hPTH(1–34) (25 μg/kg, SC, three times per week), or vehicle (NS; 1 ml/kg, three times per week). Groups of animals were killed after days 60 and 180 of treatment, and either the proximal tibial metaphysis or lumbar vertebral body were studied. Bone volume and architecture were assessed by μCT and histomorphometry. Measurements of bone quality included the degree of bone mineralization (DBM), localized elastic modulus, bone turnover by histomorphometry, compression testing of the LVB, and three-point bending testing of the femur. The trabecular bone volume, DBM, elastic modulus, and compressive bone strength were all significantly lower at day 60 post-OVX (pretreatment, day 0 study) than at baseline. After 60 days of all of the bone active treatments, bone mass and material measurements agent were restored. However, after 180 days of treatment, the OVX + PTH group further increased BV/TV (+30% from day 60, p &lt; 0.05 within group and between groups). In addition, after 180 days of treatment, there was more highly mineralized cortical and trabecular bone and increased cortical bone size and whole bone strength in OVX + PTH compared with other OVX + antiresorptives. Treatment of estrogen-deficient aged rats with either antiresorptive agents or PTH rapidly improved many aspects of bone quality including microarchitecture, bone mineralization, turnover, and bone strength. However, prolonged treatment for 180 days with PTH resulted in additional gains in bone quality and bone strength, suggesting that the maximal gains in bone strength in cortical and trabecular bone sites may require a longer treatment period with PTH.
Der Opfer-Plot. Probleme und neue Felder der deutschen Arbeitsmigrationsforschung
Authors: Richter Hedwig , Richter Ralf 
Published: January 2009

Vorspann
Sind die teils massiven Integrationsprobleme der Arbeitsmigranten nur die Folge falschen Zögerns der Bundesregierungen, Deutschland schon in den 1960er und 1970er Jahren zum Einwanderungsland zu erklären? Gegen den in der Ideologisierung nach 1968 entstandenen „Opfer-Plot“ von der kapitalistischen Ausbeutung der Migranten – im „Spiegel“ erst kürzlich wieder ausgebreitet – wenden sich Hedwig und Ralf Richter. Auf der Basis ihrer Studien über italienische Gastarbeiter im Wolfsburger VW-Werk plädieren die Autoren dafür, die Mitverantwortung der Migranten für das Geschehen stärker zu berücksichtigen und auf diachrone Vergleiche mit der Fremdarbeiterpolitik der NS-Zeit besser zu verzichten.
Retinoic acid receptor and retinoid X receptor subtype expression for the differential diagnosis of thyroid neoplasms
Authors: Hoftijzer Hendrieke C , Liu Ying Y , Morreau Hans , van Wezel Ton , Pereira Alberto M , Corssmit Eleonora P M , Romijn Johannes A , Smit Johannes W A 
Published: January 2009

BackgroundAlthough differential expression of retinoic acid receptor (RAR) subtypes between benign and malignant thyroid tissues has been described, their diagnostic value has not been reported.AimTo investigate the diagnostic accuracy of RAR and retinoid X receptor (RXR) subtype protein expression for the differential diagnosis of thyroid neoplasms.MethodsWe used a tissue array containing 93 benign thyroid tissues (normal thyroid, multinodular goiter, and follicular adenoma (FA)) and 77 thyroid carcinomas (papillary thyroid carcinoma (PTC), follicular thyroid carcinoma, and follicular variant of PTC (FVPTC)). Immunostaining was done for RAR and RXR subtypes. Staining was analyzed semiquantitatively based on receiver operating curve analyses and using hierarchical cluster analysis.ResultsWe found increased expression of cytoplasmic (c) RARA, cRARG, cRXRB and decreased expression of nuclear (n) RARB, nRARG, and nRXRA in thyroid carcinomas compared with benign tissues. We found three proteins differently expressed between FA and FTC and five proteins differentially expressed between FA and FVPTC, with high diagnostic accuracies. Using cluster analysis, the combination of negative staining of membranous RXRB and positive staining for cRXRB had a high positive predictive value (98%) for malignant thyroid disease, whereas the combination of positive nRXRA and negative cRXRB staining had a high predictive value (91%) for benign thyroid lesions.ConclusionWe conclude that differences in RAR and RXR subtype protein expression may be valuable for the differential diagnosis of thyroid neoplasms. The results of this study and especially the value of cluster analysis have to be confirmed in subsequent studies.
CLEAR LAKE SEDIMENTS: ANTHROPOGENIC CHANGES IN PHYSICAL SEDIMENTOLOGY AND MAGNETIC RESPONSE
Authors: Osleger David A. , Zierenberg Robert A. , Suchanek Thomas H. , Stoner Joseph S. , Morgan Sally , Adam David P. 
Published: January 2009

We analyzed the sedimentological characteristics and magnetic properties of cores from the three basins of Clear Lake, California, USA, to assess the depositional response to a series of land use changes that occurred in the watershed over the 20th century. Results indicate that distinct and abrupt shifts in particle size, magnetic concentration/mineralogy, and redox conditions occur concurrently with a variety of ecological and chemical changes in lake bed sediments. This coincidence of events occurred around 1927, a datum determined by an abrupt increase in total mercury (Hg) in Clear Lake cores and the known initiation of open‐pit Hg mining at the Sulphur Bank Mercury Mine, confirmed by 210Pb dating. Ages below the 1927 horizon were determined by accelerator mass spectrometry on 14C of coarse organic debris. Calculated sedimentation rates below the 1927 datum are ∼1 mm/yr, whereas rates from 1927 to 2000 are up to an order of magnitude higher, with averages of ∼3.5–19 mm/yr.In both the Oaks and Upper Arms, the post‐1927 co‐occurrence of abrupt shifts in magnetic signatures with color differences indicative of changing redox conditions is interpreted to reflect a more oxygenated diagenetic regime and rapid burial of sediment below the depth of sulfate diffusion. Post‐1927 in the Oaks Arm, grain size exhibits a gradual coarsening‐upward pattern that we attribute to the input of mechanically deposited waste rock related to open‐pit mining activities at the mine. In contrast, grain size in the Upper Arm exhibits a gradational fining‐upward after 1927 that we interpret as human‐induced erosion of fine‐grained soils and chemically weathered rocks of the Franciscan Assemblage by heavy earthmoving equipment associated with a road‐ and home‐building boom, exacerbated by stream channel mining and wetlands destruction. The flux of fine‐grained sediment into the Upper Arm increased the nutrient load to the lake, and that in turn catalyzed profuse cyanobacterial blooms through the 20th century. The resulting organic biomass, in combination with the increased inorganic sediment supply, contributed to the abrupt increase in sedimentation rate after 1927.
Assessing the importance of dietborne cadmium and particle characteristics on bioavailability and bioaccumulation in the nematodeCaenorhabditis elegans
Authors: Offermann Kirsten , Matthäi Anett , Ahlf Wolfgang 
Published: January 2009

AbstractTo investigate the impact of multiple cadmium exposure pathways on bioavailability and bioaccumulation, Caenorhabditis elegans were exposed to either dissolved cadmium or to both particulate (dietborne) and dissolved cadmium. The dietborne metal exposure scenarios included evaluations of two bacteria species (Escherichia coli and Arthrobacter globiformis), varying bacteria concentrations, artificial particles (with different surface functional groups or coated with lipopolysaccharides), as well as a mixture of both bacteria and artificial particles. Total cadmium concentration in the nematodes was used as a proxy for total metal accumulation. Internal cadmium availability was determined using the cadmium-responsive gene-1 (cdr-1) transcript level, as quantified by real-time polymerase chain reaction. Particle-associated exposure was the predominant contributor to cadmium bio-availability and bioaccumulation; however, no clear relationship between cdr-1 expression and cadmium body burden was observed. The quality and quantity of particles, rather than the total particle-associated metal load, was of primary importance in regulating cadmium uptake and accumulation. Nematodes exposed to cadmium-contaminated bacteria displayed significantly higher levels of cdr-1 expression relative to artificial particles. Furthermore, C. elegans displayed a statistically significant difference in cadmium accumulation patterns between the biotic diet, abiotic particles, and aqueous phase. Waterborne exposure caused an increase in total cadmium body burden following inhibition of the P-glycoprotein transport system in nematodes. This may suggest that cadmium taken up by the aqueous phase is detoxified preferentially by excretion.
Mass recovery methods for trichloroethylene in plant tissue
Authors: Gopalakrishnan Gayathri , Werth Charles J. , Negri Maria Cristina 
Published: January 2009

AbstractMonitoring expenses form a significant fraction of the costs associated with remediation of contaminated soil and groundwater sites. A novel monitoring method that could result in significant cost savings is the use of plants as monitoring devices; previous work indicates that plant tissue samples, especially trunk (core) and branch samples, can be used to delineate soil and groundwater plumes at phytoremediation sites. An important factor in reducing the uncertainty associated with this sampling method is development of a technique to analyze, both consistently and accurately, the chemicals stored in plant tissue samples. The present research presents a simple, robust, and inexpensive technique to recover most of the contaminant in plant branch tissue, irrespective of the age or species of the plant. Tricholoroethylene (TCE) was the chemical analyzed. A number of headspace and solvent extraction techniques in the literature were evaluated, including headspace extraction at different incubation times and temperatures and solvent extraction using hexane or hot methanol. Extraction using hot methanol was relatively fast, simple, and reliable; this method recovered more than 89% of the TCE present in branches of five different tree species.
Perspectives on the Etiology of Chronic Rhinosinusitis: An Immune Barrier Hypothesis
Authors: Kern Robert C. , Conley David B. , Walsh William , Chandra Rakesh , Kato Atsushi , Tripathi-Peters Anju , Grammer Leslie C. , Schleimer Robert P. 
Published: January 2009

Background Chronic rhinosinusitis (CRS) has been defined as persistent symptomatic inflammation of the nasal and sinus mucosa resulting from the interaction of multiple host and environmental factors. Recent studies have implicated Alternaria fungi or toxigenic Staphylococcus aureus as critical agents in CRS pathogenesis. The emphasis on environmental agents in CRS etiology has focused interest toward elimination of those agents as the prime mechanism of therapy. This viewpoint is in marked contrast to the current perspective on some other chronic inflammatory epithelial disorders that afflict the skin, lungs, and gut, wherein host factors are believed to predispose to disease expression in the presence of ubiquitous environmental agents. Methods The current review evaluates CRS etiology from this perspective and considers that CRS develops, in part, as an outcome of a dysfunctional host response. Specifically, evidence from our laboratory and others will be reviewed indicating that CRS is associated with a failure of the mechanical and immunologic barriers across the nasal mucosa. The hypothesis would further propose that genetic and epigenetic variation predisposes susceptible individuals to barrier failure in the presence of environmental stress leading to CRS. Results From this unifying perspective, bacteria and fungi are seen as disease modifiers rather than primary etiologic agents. Conclusion The goal is to place concepts of CRS pathophysiology in a framework consistent with a current understanding of chronic inflammation in general and epithelial disease in particular.