bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental and theoretical model of microvascular network remodeling and blood flow redistribution following minimally invasive microvessel laser ablation
Authors: Gruionu Gabriel , Baish James , McMahon Sean , Blauvelt David , Gruionu Lucian G. , Lenco Mara Onita , Vakoc Benjamin J. , Padera Timothy P. , Munn Lance L. 
Published: April 2024

AbstractOverly dense microvascular networks are treated by selective reduction of vascular elements. Inappropriate manipulation of microvessels could result in loss of host tissue function or a worsening of the clinical problem. Here, experimental, and computational models were developed to induce blood flow changes via selective artery and vein laser ablation and study the compensatory collateral flow redistribution and vessel diameter remodeling. The microvasculature was imaged non-invasively by bright-field and multi-photon laser microscopy, and optical coherence tomography pre-ablation and up to 30 days post-ablation. A theoretical model of network remodeling was developed to compute blood flow and intravascular pressure and identify vessels most susceptible to changes in flow direction. The skin microvascular remodeling patterns were consistent among the five specimens studied. Significant remodeling occurred at various time points, beginning as early as days 1–3 and continuing beyond day 20. The remodeling patterns included collateral development, venous and arterial reopening, and both outward and inward remodeling, with variations in the time frames for each mouse. In a representative specimen, immediately post-ablation, the average artery and vein diameters increased by 14% and 23%, respectively. At day 20 post-ablation, the maximum increases in arterial and venous diameters were 2.5× and 3.3×, respectively. By day 30, the average artery diameter remained 11% increased whereas the vein diameters returned to near pre-ablation values. Some arteries regenerated across the ablation sites via endothelial cell migration, while veins either reconnected or rerouted flow around the ablation site, likely depending on local pressure driving forces. In the intact network, the theoretical model predicts that the vessels that act as collaterals after flow disruption are those most sensitive to distant changes in pressure. The model results correlate with the post-ablation microvascular remodeling patterns.
Biogeochemical dynamics in a marine storm demonstrates differences between natural and anthropogenic impacts
Authors: Tiano Justin , Witbaard Rob , Gerkema Theo , Soetaert Karline 
Published: April 2024

AbstractThis study explores the impact of a wind storm on sediment resuspension and marine biogeochemical dynamics. Additionally, the storm took place during an expedition researching bottom trawling, enabling the direct comparison of certain natural and fisheries-related disturbances. The storm was initiated by a decline in atmospheric pressure and a 2 h period of gale force winds, which was followed by over 40 h of elevated bottom currents. Storm induced turbidity, potentially a cumulative post-fishing impact, was remarkably higher compared to what was observed in a recent trawling event. Storm-induced mixing and movement of water masses led to decreased silicate and increased phosphate concentrations in the water column, accompanied by lower salinity and higher fluorescence. The erosion depth of the seabed averaged around 0.3 cm during the peak turbidity period. Trawl-induced erosion in the area has been measured at over twice that depth, and has been linked to intermittent reductions in near-bed oxygen levels. In contrast, storm-induced turbidity coincided with increased oxygen due to wave mixing, suggesting inherent differences in how trawling and storms can oxidize reduced substances. These findings suggest that storms have a greater regional impact, whereas the local impacts of bottom trawling on biogeochemistry can be more significant.
Optimized reusable modular 3D-printed models of choledochal cyst to simulate laparoscopic and robotic bilioenteric anastomosis
Authors: Gu Jing , Cao Jie , Cao Wenli , Chen Yusuo , Wei Fangqiang 
Published: April 2024

AbstractLaparoscopic and robotic surgery is a challenge to the surgeon's hand–eye coordination ability, which requires constant practice. Traditional mentor training is gradually shifting to simulation training based on various models. Laparoscopic and robotic bilioenteric anastomosis is an important and difficult operation in hepatobiliary surgery. We constructed and optimized the reusable modular 3D-printed models of choledochal cyst. The aim of this study was to verify the ability of this optimized model to distinguish between surgeons with different levels of proficiency and the benefits of repeated practice. A total of 12 surgeons with different levels participated in the study. Operation completion time and OSATS score were recorded. The model was validated by Likert scale. Surgeons were shown the steps and contents before performing laparoscopic or robotic bilioenteric anastomosis using the model. Surgeons with different levels of experience showed different levels when performing laparoscopic bilioenteric anastomosis on this model. Repeated training can significantly shorten the time of laparoscopic bilioenteric anastomosis and improve the operation scores of surgeons with different levels of experience. At the same time, preliminary results have shown that the performance of surgeons on the domestic robotic platform was basically consistent with their laparoscopic skills. This model may distinguish surgeons with different levels of experience and may improve surgical skills through repeated practice. It is worth noting that in order to draw more reliable conclusions, more subjects should be collected and more experiments should be done in the future.
Discrimination of distinct chicken M cell subsets based on CSF1R expression
Authors: Zeinali Safieh , Sutton Kate , Zefreh Masoud Ghaderi , Mabbott Neil , Vervelde Lonneke 
Published: April 2024

AbstractIn mammals, a subset of follicle-associated epithelial (FAE) cells, known as M cells, conduct the transcytosis of antigens across the epithelium into the underlying lymphoid tissues. We previously revealed that M cells in the FAE of the chicken lung, bursa of Fabricius (bursa), and caecum based on the expression of CSF1R. Here, we applied RNA-seq analysis on highly enriched CSF1R-expressing bursal M cells to investigate their transcriptome and identify novel chicken M cell-associated genes. Our data show that, like mammalian M cells, those in the FAE of the chicken bursa also express SOX8, MARCKSL1, TNFAIP2 and PRNP. Immunohistochemical analysis also confirmed the expression of SOX8 in CSF1R-expressing cells in the lung, bursa, and caecum. However, we found that many other mammalian M cell-associated genes such as SPIB and GP2 were not expressed by chicken M cells or represented in the chicken genome. Instead, we show bursal M cells express high levels of related genes such as SPI1. Whereas our data show that bursal M cells expressed CSF1R-highly, the M cells in the small intestine lacked CSF1R and both expressed SOX8. This study offers insights into the transcriptome of chicken M cells, revealing the expression of CSF1R in M cells is tissue-specific.
Comprehensive geriatric assessment of older patients with renal disease: a cross-sectional survey
Authors: Chen Xiaoyu , Hu Yingchun , Peng Lei , Wu Hongmei , Ren Jiangwen , Liu Guanjian , Cao Li , Yang Ming , Hao Qiukui 
Published: April 2024

AbstractMultidimensional health function impairments are common in older patients with chronic kidney disease (CKD). The purpose of this study was to explore whether the risk or severity of geriatric syndrome increased with a decline in renal function. This survey was conducted for CKD patients aged  ≥ 60 years and hospitalized at West China Hospital of Sichuan University (Center of Gerontology and Geriatrics, Nephrology, and Endocrinology) and Chengdu Kangfu Kidney Disease Hospital from September 01, 2013 to June 30, 2014. Patients underwent multidimensional individualized assessments by trained doctors. Logistic regression analysis found that the risk of assisted walking (P = 0.001) and urinary incontinence (P = 0.039) increased with a decline in renal function. Regression analysis revealed that the scores of activities of daily living (P = 0.024), nutritional status (P = 0.000), total social support (P = 0.014), and objective support (P = 0.000) decreased with a decline in renal function.
Parameter optimization of vibration control system for adjacent building structures based on negative stiffness inerter damper
Authors: Kang Xiaofang , Tang Jianjun , Wei Jiachen , Jiang Xueqin , Sheng Ziyi , Shi Xianzeng 
Published: April 2024

AbstractBuilding structures are subjected to strong earthquakes, which result in lateral collisions between them. Such collisions often cause severe structural damage and exacerbate the seismic hazard risk of building structures during earthquake events. This paper discusses the application of vibration control devices based on negative stiffness inerter damper in single-story adjacent building structures. The dynamic equations of the vibration control system containing different types of negative stiffness inerter damper under seismic excitation are established as a unified model. The H2 norm theory and Monte Carlo pattern search method are used to optimize the design parameters to improve the vibration control performance of the system, and the dynamic characteristics of the system are investigated. The results demonstrate that attaching negative stiffness inerter damper to adjacent building structures can effectively improve the overall seismic capacity reserve of the building and reduce the risk of collision of adjacent building structures; improve the robustness and stability of the system, and better reduce the displacement response of the building structure under seismic excitation. In addition, the potential of NSID-based vibration control devices to convert seismic energy into usable electricity has been investigated.
EPAS1 expression contributes to maintenance of the primordial follicle pool in the mouse ovary
Authors: Martin Jacinta H. , Bernstein Ilana R. , Lyons Jess M. , Brady Ariel R. , Mabotuwana Nishani S. , Stanger Simone J. , De Oliveira Camila Salum , Damyanova Katerina B. , Nixon Brett , Lord Tessa 
Published: April 2024

AbstractOxygen availability can have profound effects on cell fate decisions and survival, in part by regulating expression of hypoxia-inducible factors (HIFs). In the ovary, HIF expression has been characterised in granulosa cells, however, any requirement in oocytes remains relatively undefined. Here we developed a Hif2a/Epas1 germline-specific knockout mouse line in which females were fertile, however produced 40% fewer pups than controls. No defects in follicle development were detected, and quality of MII oocytes was normal, as per assessments of viability, intracellular reactive oxygen species, and spindle parameters. However, a significant diminishment of the primordial follicle pool was evident in cKO females that was attributed to accelerated follicle loss from postnatal day 6 onwards, potentially via disruption of the autophagy pathway. These data demonstrate the importance of HIF signalling in oocytes, particularly at the primordial follicle stage, and lend to the importance of controlling oxygen tension in the development of in vitro growth and maturation approaches for assisted reproduction.
Study on the detonation wave propagation of shaped charge with three-layer liner and its driving characteristics to liner
Authors: Hao Zhiwei , Wang Zhijun , Xu Yongjie , Duan Conghui , Wang Yifan 
Published: April 2024

AbstractWith the continuous improvement of various armor protection technologies, the armor protection performance has increased significantly, and then the damage performance requirements of armor-piercing ammunition have also increased. In order to improve the penetration ability of the liner, a new three-layer liner structure is designed in this paper. The jet forming process was simulated by AUTODYN software. The mechanism of shaped jet forming of three-layer liner was studied. The reason why the penetration depth of three-layer liner was higher than that of ordinary liner was explained. The influence of three-layer liner on the propagation of detonation wave and the change of pressure when detonation wave acted on liner were found, which provided a new idea for improving the penetration depth of jet. The influence of liner material, cone angle and stand-off on jet forming and penetration was also studied by orthogonal optimization experiment, and the structural parameters with the best penetration performance were obtained. The results show that the pressure at the convergence point increases first and then decreases during the formation of the jet of the three-layer liner. The pressure at the convergence point when the three-layer liner material is from low impedance to high impedance from the outside to the inside is much larger than the pressure at the convergence point from high impedance to low impedance. When the three-layer liner material is Al 2024-Copper–Tantalum from the outside to the inside, the pressure at the convergence point of the three-layer liner at different times is higher than that of the double-layer liner and the single-layer liner. Reasonable matching of different impact impedance materials in the three-layer liner can greatly improve the pressure value of the detonation wave acting on the cone liner. The maximum pressure at the convergence point on the axis is 39.10 GPa, which is 22.00% higher than that of the double-layer liner at the convergence point, and 53.03% higher than that of the single-layer liner at the convergence point. The orthogonal design test scheme is simulated and analyzed. The penetration depth is taken as the observation index, and the range analysis is adopted. The results show that the material matching of the three-layer liner has the greatest influence on the depth of the jet penetrating the target plate, followed by the cone angle of the three-layer liner. Relatively speaking, the stand-off has the least influence on the result. Reasonable matching of materials with different impact impedances in the three-layer liner can maximize the penetration depth of the jet into the target plate.
Influencing factors on the time to CT in suspected pulmonary embolism: an explorative investigation
Authors: Koehler Daniel , Ozga Ann-Kathrin , Molwitz Isabel , Shenas Farzad , Keller Sarah , Adam Gerhard , Yamamura Jin 
Published: April 2024

AbstractPulmonary embolism is a potentially fatal condition with increased mortality if anticoagulation is delayed. This study aimed to find influencing factors on the duration from requesting a computed tomography (CT) pulmonary angiography (CTPA) to performing a CTPA in suspected acute pulmonary embolism. In 1849 cases, automatically generated time data were extracted from the radiological information system. The impact of the distance to the scanner, case-related features (sector of patient care, triage), and workload (demand for CTs, performed CTs, available staff, hospital occupancy) were investigated retrospectively using multiple regression. The time to CTPA was shorter in cases from the emergency room (ER) than in inpatients and outpatients at distances below 160 m and 240 m, respectively. While requests from the ER were also performed faster than cases from regular wards (< 180 m), no difference was found between the ER and intensive care units. Compared to “not urgent” cases, the workflow was shorter in “urgent” (− 17%) and “life-threatening” (− 67%) situations. The process was prolonged with increasing demand (+ 5%/10 CTs). The presented analysis identified relevant in-hospital influences on the CTPA workflow, including the distance to the CT together with the sector of patient care, the case triage, and the demand for imaging.
Radiological manifestations and clinical findings of patients with oncologic and osteoporotic medication-related osteonecrosis of the jaw
Authors: Shin Jeong Won , Kim Jo-Eun , Huh Kyung-Hoe , Yi Won-Jin , Heo Min-Suk , Lee Sam-Sun , Choi Soon-Chul 
Published: April 2024

AbstractMedication-related osteonecrosis of the jaw (MRONJ) poses a challenging form of osteomyelitis in patients undergoing antiresorptive therapies in contrast to conventional osteomyelitis. This study aimed to compare the clinical and radiological features of MRONJ between patients receiving low-dose medications for osteoporosis and those receiving high-dose medications for oncologic purposes. The clinical, panoramic radiographic, and computed tomography data of 159 patients with MRONJ (osteoporotic group, n = 120; oncologic group, n = 39) who developed the condition after using antiresorptive medications for the management of osteoporosis or bone malignancy were analyzed. The osteoporotic group was older (75.8 vs. 60.4 years, p < 0.01) and had a longer duration of medication usage than the oncologic group (58.1 vs. 28.0 months, p < 0.01). Pus discharge and swelling were more common in the osteoporotic group (p < 0.05), whereas bone exposure was more frequent in the oncologic group (p < 0.01). The mandibular cortical index (MCI) in panoramic radiographs was higher in the osteoporotic group (p < 0.01). The mean sequestra size was larger in the oncologic group than in the osteoporotic group (15.3 vs. 10.6 mm, p < 0.05). The cured rate was significantly higher in the osteoporotic group (66.3% vs. 33.3%, p < 0.01). Oncologic MRONJ exhibited distinct clinical findings including rapid disease onset, fewer purulent signs, and lower cure rates than osteoporotic MRONJ. Radiological features such as sequestrum size on CT scan, and MCI values on panoramic radiographs, may aid in differentiating MRONJ in osteoporotic and oncologic patients.
Unraveling the Indian monsoon’s role in fueling the unprecedented 2022 Marine Heatwave in the Western North Pacific
Authors: Song Qianghua , Wang Chunzai , Yao Yulong , Fan Hanjie 
Published: April 2024

AbstractAn unprecedented marine heatwave (MHW) event occurred in the middle-high latitudes of the western North Pacific during the summer of 2022. We demonstrate that excessive precipitation thousands of kilometers away fuels this extreme MHW event in July 2022. In the upper atmosphere, a persistent atmospheric blocking system, forming over the MHW region, reduces cloud cover and increases shortwave radiation at the ocean surface, leading to high sea surface temperatures. Atmospheric perturbations induced by latent heat release from the extreme precipitation in the Indian summer monsoon region enhance this atmospheric blocking through the propagation of quasi-stationary Rossby waves. Our hypothesis is verified by using a numerical model that is forced with the observed atmospheric anomalous diabatic heating. This study sheds light on how a subtropical extreme event can fuel another extreme event at middle-high latitudes through an atmospheric teleconnection.
Structural dynamics of Na+ and Ca2+ interactions with full-size mammalian NCX
Authors: Giladi Moshe , Fojtík Lukáš , Strauss Tali , Da’adoosh Benny , Hiller Reuben , Man Petr , Khananshvili Daniel 
Published: April 2024

AbstractCytosolic Ca2+ and Na+ allosterically regulate Na+/Ca2+ exchanger (NCX) proteins to vary the NCX-mediated Ca2+ entry/exit rates in diverse cell types. To resolve the structure-based dynamic mechanisms underlying the ion-dependent allosteric regulation in mammalian NCXs, we analyze the apo, Ca2+, and Na+-bound species of the brain NCX1.4 variant using hydrogen-deuterium exchange mass spectrometry (HDX-MS) and molecular dynamics (MD) simulations. Ca2+ binding to the cytosolic regulatory domains (CBD1 and CBD2) rigidifies the intracellular regulatory loop (5L6) and promotes its interaction with the membrane domains. Either Na+ or Ca2+ stabilizes the intracellular portions of transmembrane helices TM3, TM4, TM9, TM10, and their connecting loops (3L4 and 9L10), thereby exposing previously unappreciated regulatory sites. Ca2+ or Na+ also rigidifies the palmitoylation domain (TMH2), and neighboring TM1/TM6 bundle, thereby uncovering a structural entity for modulating the ion transport rates. The present analysis provides new structure-dynamic clues underlying the regulatory diversity among tissue-specific NCX variants.
Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo
Authors: Tsuchiya Masaki , Tachibana Nobuhiko , Hamachi Itaru 
Published: April 2024

AbstractCellular glucose uptake is a key feature reflecting metabolic demand of cells in physiopathological conditions. Fluorophore-conjugated sugar derivatives are widely used for monitoring glucose transporter (GLUT) activity at the single-cell level, but have limitations in in vivo applications. Here, we develop a click chemistry-based post-labeling method for flow cytometric measurement of glucose uptake with low background adsorption. This strategy relies on GLUT-mediated uptake of azide-tagged sugars, and subsequent intracellular labeling with a cell-permeable fluorescent reagent via a copper-free click reaction. Screening a library of azide-substituted monosaccharides, we discover 6-azido-6-deoxy-D-galactose (6AzGal) as a suitable substrate of GLUTs. 6AzGal displays glucose-like physicochemical properties and reproduces in vivo dynamics similar to 18F-FDG. Combining this method with multi-parametric immunophenotyping, we demonstrate the ability to precisely resolve metabolically-activated cells with various GLUT activities in ex vivo and in vivo models. Overall, this method provides opportunities to dissect the heterogenous metabolic landscape in complex tissue environments.
Isolated flat band in artificially designed Lieb lattice based on macrocycle supramolecular crystal
Authors: Chen Cheng-Yi , Li En , Xie Huilin , Zhang Jianyu , Lam Jacky Wing Yip , Tang Ben Zhong , Lin Nian 
Published: April 2024

AbstractIsolated flat bands are known to host various strongly correlated phases due to the enhanced Coulomb interactions when the flat bands are gapped from dispersive bands. One way to achieve an isolated flat band is by breaking the on-site energy symmetry in a Lieb lattice. In this study, we demonstrate the design of such a Lieb lattice. The self-assembly of square-shaped macrocycle molecules on a Ag(111) surface forms a two-dimensional supramolecular crystal, comprising three types of nanopores with different sizes arranged in a Lieb lattice. The surface-state electrons of the Ag(111) substrate confined by these nanopores behave as quantum dots with specific energies depending on the pore size. Using scanning tunneling spectroscopy and plane-wave quantum simulation, we reveal that this artificial Lieb lattice exhibits an isolated flat band gapped at 0.16 eV from the nearest band. The supramolecular crystal is nearly defect-free and extends to sub-micrometer size, making it a practical platform for exploring the exotic properties of the isolated flat band.