bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expressionin planta
Authors: Farre Maria Font , Brown Daniel , Toth Reka , Mahadevan Chidambareswaren , Brazier-Hicks Melissa , Morimoto Kyoko , Kaschani Farnusch , Sinclair John , Dale Richard , Hall Samantha , Morris Melloney , Kaiser Markus , Wright Aaron T. , Burton Jonathan , van der Hoorn Renier A. L. 
Published: March 2024

AbstractEukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes. Identification by mass spectrometry revealed labeling of a wide range of active P450s, including six plant P450s, 40 mouse P450s and 13 P450s of the fungal wheat pathogenZymoseptoria tritici. We next used transient expression of GFP-tagged P450s by agroinfiltration to show ER-targeting and NADPH-dependent, activity-based labeling of plant, mouse and fungal P450s. Both global profiling and transient expression can be used to detect a broad range of active P450s to study e.g. their regulation and discover selective inhibitors.
Multi-layered Network Analysis of Osteoking in the Treatment of Osteoporosis: Unraveling Mechanisms from Gene Expression to Molecular Docking
Authors: Chen He , Ying Jun , Xie Xianjie , Huang Boyun , Lin Pengcheng 
Published: March 2024

AbstractThis study aimed to elucidate the therapeutic mechanisms of Osteoking in the treatment of osteoporosis through a comprehensive analysis of potential targets, active ingredients, and associated pathways.MethodThe study employed an integrated approach to understand the molecular mechanisms underlying Osteoking’s treatment of osteoporosis. The construction of the protein-protein interaction network involved analyzing data from GENEMANIA and STRING databases. KEGG enrichment analysis was performed to identify enriched pathways, focusing on the cAMP signaling and PI3K-AKT signaling pathways. Active ingredients, disease targets, and osteoporosis-related pathways were integrated into a comprehensive network diagram using Cytoscape.ResultThe Gene Expression Omnibus (GEO) database was employed to identify osteoporosis-related gene targets, revealing 3,578 downregulated and 1,204 upregulated genes. Simultaneously, Osteoking’s active ingredients and potential targets were investigated using the Traditional Chinese Medicine Integrated Database (ETCM). A protein-protein interaction network and KEGG pathway enrichment analysis were constructed, highlighting potential targets for Osteoking’s therapeutic effects on osteoporosis. The study also conducted molecular docking analysis, revealing the strong binding capacities of Kaempferol with key disease targets. The results suggest that Osteoking, particularly its active component Kaempferol, holds promise as a potential intervention for osteoporosis, providing insights for further exploration and development of osteoporosis treatmentsConclusionsIn conclusion, despite some limitations, this study provides valuable information for the treatment of osteoporosis. Future research should make further progress by continually expanding data sources, conducting in-depth experimental validations, and broadening the scope of targets to better understand and address this common skeletal disorder.FundingThis work was supported by the Scientific Foundation of Fuzhou Municipal Health Commission (2021-S-wp3).
Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance inToxoplasma gondii
Authors: Souza Rodolpho Ornitz Oliveira , Yang Chunlin , Arrizabalaga Gustavo 
Published: March 2024

ABSTRACTThe single mitochondrion of the obligate intracellular parasiteToxoplasma gondiiis highly dynamic. Toxoplasma’s mitochondrion changes morphology as the parasite moves from the intracellular to the extracellular environment and during division. Toxoplasma’s mitochondrial dynamic is dependent on an outer mitochondrion membrane-associated protein LMF1 and its interaction with IMC10, a protein localized at the inner membrane complex (IMC). In the absence of either LMF1 or IMC10, parasites have defective mitochondrial morphology and inheritance defects. As little is known about mitochondrial inheritance inToxoplasma, we have used the LMF1/IMC10 tethering complex as an entry point to dissect the machinery behind this process. Using a yeast two-hybrid screen, we previously identified Myosin A (MyoA) as a putative interactor of LMF1. Although MyoA is known to be located at the parasite’s pellicle, we now show through ultrastructure expansion microscopy (U-ExM) that this protein accumulates around the mitochondrion in the late stages of parasite division. Parasites lacking MyoA show defective mitochondrial morphology and a delay in mitochondrion delivery to the daughter parasite buds during division, indicating that this protein is involved in organellar inheritance. Disruption of the parasite’s actin network also affects mitochondrion morphology. We also show that parasite-extracted mitochondrion vesicles interact with actin filaments. Interestingly, mitochondrion vesicles extracted out of parasites lacking LMF1 pulled down less actin, showing that LMF1 might be important for mitochondrion and actin interaction. Accordingly, we are showing for the first time that actin and Myosin A are important forToxoplasmamitochondrial morphology and inheritance.
The green library revolution: a catalyst for climate change action
Authors: Ajani Yusuf Ayodeji , Tella Adeyinka , Enakrire Rexwhite Tega 
Published: March 2024

Purpose
Libraries are currently undergoing a significant transformation, emerging as dynamic advocates for environmental sustainability, surpassing their conventional roles as mere repositories of knowledge. In today’s world, dominated by the growing climate crisis, these evolving institutions are poised to play a pivotal role in addressing climate change. However, they confront a range of challenges, including the need to establish sustainable operations, provide accessible climate information, engage diverse audiences and uphold ethical standards.

Design/methodology/approach
The research methodology used for this study involves an extensive review of literature. The authors analyze existing studies concerning green libraries and their role as catalysts for climate change action globally. This analysis draws from a variety of scholarly sources, encompassing articles, books and reports, to provide a comprehensive overview of the subject.

Findings
The findings indicate that despite the aforementioned challenges, libraries in developed countries worldwide have demonstrated noteworthy success through initiatives such as green building projects, community engagement programs, collaborative partnerships, expansion of digital resources, innovative climate literacy initiatives and a commitment to ethical stewardship. These achievements serve as a source of inspiration, fostering hope and spurring action as libraries empower communities to embrace environmental stewardship. They showcase the potential of libraries as catalysts for positive environmental change.

Originality/value
The originality and value of this research lie in its insights into the potential of green libraries initiative as a catalyst for climate change action. Ultimately, this study opens the door to untold literary exploration, revolutionizing the art of green library capacity in the ever-evolving technological landscape. To augment their impact, libraries are encouraged to broaden the scope of their climate education initiatives, expand their digital resources, cultivate strategic collaborations, prioritize ethical responsibility and implement thorough evaluation and communication practices.
Debt, economic growth and threshold effects: Evidence from developing countries
Authors: Ibañez Martín María María , Rojas Mara Leticia , Dabús Carlos 
Published: March 2024

PurposeMost empirical papers on threshold effects between debt and growth focus on developed countries or a mix of developing and developed economies, often using public debt. Evidence for developing economies is inconclusive, as is the analysis of other threshold effects such as those probably caused by the level of relative development or the repayment capacity. The objective of this study was to examine threshold effects for developing economies, including external and total debt, and identify them in the debt-growth relation considering three determinants: debt itself, initial real Gross Domestic Product (GDP) per capita and debt to exports ratio.Design/methodology/approachWe used a panel threshold regression model (PTRM) and a dynamic panel threshold model (DPTM) for a sample of 47 developing countries from 1970 to 2019.FindingsWe found (1) no evidence of threshold effects applying total debt as a threshold variable; (2) one critical value for external debt of 42.32% (using PTRM) and 67.11% (using DPTM), above which this factor is detrimental to growth; (3) two turning points for initial GDP as a threshold variable, where total and external debt positively affects growth at a very low initial GDP, it becomes nonsignificant between critical values, and it negatively influences growth above the second threshold; (4) one critical value for external debt to exports using PTRM and DPTM, below which external debt positively affects growth and negatively above it.Originality/valueThe outcome suggests that only poorer economies can leverage credits. The level of the threshold for the debt to exports ratio is higher than that found in previous literature, implying that the external restriction could be less relevant in recent periods. However, the threshold for the external debt-to-GDP ratio is lower compared to previous evidence.
Leveraging artificial intelligence in firm-generated online customer communities: a framework and future research agenda
Authors: Marti Candice L. , Liu Huimin , Kour Gurpreet , Bilgihan Anil , Xu Yu 
Published: March 2024

PurposeIn an era where complex technological advances increasingly govern service delivery, it is incumbent on service firms to pioneer innovative strategies to sustain customer engagement and cultivate loyalty. This conceptual paper examines the transformative potential of artificial intelligence (AI) in the realm of online customer communities, with a particular focus on its creation, management and enhancement facets. The authors explore how AI can revolutionize the dynamics of customer interaction, feedback mechanisms and overall engagement within the service industry.Design/methodology/approachThis conceptual paper draws from marketing and management literature focusing on customer communities and AI in service and customer engagement contexts with a robust future research agenda.FindingsA classification of online customer community engagement is provided along with a conceptual framework to guide our understanding of the integration of AI into online customer communities.Originality/valueThis exploration underscores the imperative for service firms to embrace AI-driven approaches to online customer community management, not only as a means to optimize their operations but as a vital strategy to stay competitive in the ever-evolving digital landscape. This paper examines the novel combination of AI with online customer communities and provides the framework in the form of an input-process-output (IPO) model for future research into this integration.
The end of tourism? Contemplations of collapse
Authors: Higgins-Desbiolles Freya 
Published: March 2024

PurposeThis viewpoint engages with Jem Bendell’s deep adaptation framework which was developed as a response to the threat of collapse. Proponents of deep adaptation argue that societal collapse is either likely, inevitable or already underway. The deep adaptation framework is employed as a tool to contemplate the necessary adaptation of tourism development and planning in a context of polycrisis leading to collapse.Design/methodology/approachThis is a conceptual viewpoint article that is built on deductive analysis of recent events, reports and scientific findings. It employs the deep adaptation framework to analyse possible alternative tourism futures in the face of the threat of collapse.FindingsBendell’s framework included four aspects of response to the recognition of the threat of collapse: resilience, relinquishment, restoration and reconciliation. In this work, the deep adaptation framework is employed to analyse what a deep adaptation approach to tourism might offer for efforts in securing optimal social and ecological outcomes. Findings highlight damaging activities that we should relinquish, more resilient approaches that communities could encourage and restorative practices such as rewilding and pluriversal economies as protective measures. This work recommends a precautionary approach to transform tourism education, research and practice in order to secure better tourism futures.Originality/valueThis work is novel in engaging with the threat of future collapse and in using the deep adaptation framework to consider alternative tourism futures.
Assessment of working capital management efficiency – a two-stage slack-based measure of data envelopment analysis
Authors: Seth Himanshu , Deepak Deepak , Ruparel Namita , Chadha Saurabh , Agarwal Shivi 
Published: March 2024

PurposeThis study aims to assess the efficiency of managing working capital in 1,388 Indian manufacturing firms from 2008 to 2019 and investigate the effects of firm-specific and macro-level determinants on working capital management (WCM) efficiency.Design/methodology/approachThe current study accommodates a slack-based measure (SBM) in data envelopment analysis (DEA) for computing WCM efficiency. Further, we implement a panel data fixed-effects model that controls for heterogeneity across firms in determining the relationships of selected variables with WCM efficiency.FindingsThe results highlight that manufacturing firms operate at around 50 percent efficiency, which is constant throughout the study period. Furthermore, among the selected variables, yield, earnings, age, size, ability to create internal resources, interest rate and gross domestic product (GDP) significantly affect WCM efficiency.Originality/valueInstead of the traditional models used for assessing efficiency, the SBM-DEA model is unit-invariant and monotone for slacks, implying that it can handle zero and negative data, which overcomes the incapability of prior DEA models. Hence, this provides accurate efficiency scores for robust analysis. Additionally, this paper provides a holistic working capital model recognizing firm-specific and macro-level determinants for a more explicit estimation of the relationship between WCM efficiency and the selected determinants.
Woody semi‐natural habitats modulate the effects of field size and functional crop diversity on farmland birds
Authors: Frank Claudia , Hertzog Lionel , Klimek Sebastian , Schwieder Marcel , Tetteh Gideon Okpoti , Böhner Hannah G. S. , Röder Norbert , Levers Christian , Katzenberger Jakob , Kreft Holger , Kamp Johannes 
Published: March 2024

Abstract

Agricultural intensification has simplified landscape composition and configuration, which has led to biodiversity declines. Increasing landscape‐wide crop heterogeneity can promote farmland biodiversity. However, knowledge is still lacking on how the effects of configurational and compositional crop heterogeneity (i.e. field size and crop diversity) are modulated by the amount of semi‐natural habitats in the landscape, especially across large scales.

We tested how mean field size and functional crop diversity affect farmland bird diversity and abundance over three consecutive years, and how these effects are modulated by the amount of small woody features (SWF) in the landscape. We related data from a national bird monitoring scheme to field‐level information from a novel, high‐resolution remote sensing‐based crop type map.

Smaller field sizes and higher functional crop diversity were not generally associated with a higher diversity or abundance of farmland birds. Associations varied with species' breeding habitat preferences and were modulated by the amount of SWF.

In landscapes with a low SWF amount, species diversity and the abundance of species breeding in field edges or shrubs were negatively associated with increasing field size. However, where the amount of SWF was high, larger field size was associated with higher species diversity and abundance of field and shrub breeders. Diversity increased with higher functional crop diversity, as did the abundance of non‐field breeders in landscapes with a medium to high SWF amount. Field size tended to have a stronger effect on bird diversity and abundance than functional crop diversity.

Policy implications: National and EU agricultural policies should adopt a landscape perspective by considering the amount of semi‐natural habitats when designing biodiversity‐enhancing measures that target field size and functional crop diversity. In landscapes with low SWF amount, decreasing field sizes may be particularly effective to promote farmland bird diversity and the abundance of non‐field breeders. In landscapes with a medium to high SWF amount, increasing functional crop diversity is likely more effective than reducing field sizes. Field and shrub breeders may be promoted by maintaining landscapes with large fields, only if these offer a high SWF amount, low agronomic yield potential and low productivity.
Expectations and experiences of a dance programme for autistic children: A qualitative study of parents, teachers and therapists
Authors: Golding Ali , Ambrose Zoe , Lara Joanne , Malamateniou Christina , Green Dido 
Published: March 2024

AbstractThis study explores the expectations of dance therapists/practitioners and parents and teachers of autistic children engaging in a developmental dance programme. Information gathered will support development of an evaluation tool aligned with the International Classification of Functioning Disability and Health (ICF) Core Sets for autism spectrum disorders (ASD). A qualitative study included a convenience cohort of teachers (n = 6), parents (n = 2) of children with ASD and therapists (n = 3). Three role specific focus groups were undertaken considering potential benefits and challenges of the programme. Content and thematic analysis was undertaken using NVivo12. Findings reflected four positive themes relating to behaviour, skills, social interaction and environmental supports. Therapists, teachers and parents focused differently on stereotypical and restricted behaviours, environmental supports and habits and routines respectively. These themes also emerged as challenges (to implement/achieve); with parents identifying more emotional and behavioural restrictions. A fourth challenge theme of transferability of skills emerged from teachers and therapists. Items mapped against 28 ICF Core Sets (across the lifespan) and six to ICF categories, with creativity and imagination mismatched. Findings highlight need for a specific outcome measure for dance and/or movement programmes for autistic individuals that captures meaningful functions across ICF domains for differing stakeholders.
Diagnostic challenge and survival analysis of pulmonary oligometastases and primary lung cancer in breast cancer patients
Authors: Mai Siyao , Liu Haiqing , Zeng Hong , Cheng Ziliang , Huang Jingwen , Shi Guangzi , Li Yong , Wu Zhuo 
Published: March 2024

AbstractBackgroundThe aim of this study was to compare breast cancer patients with pulmonary oligometastases (POM) and primary lung cancer (PLC) and to assess whether there were differences in clinical features, CT features, and survival outcomes between the two groups.MethodsFrom January 2010 to December 2021, the clinical records of 437 with malignant pulmonary nodules who had breast cancer patients were reviewed. POM was identified in 45 patients and PLC in 43 patients after the initial detection of pulmonary nodules. The clinicopathological characteristics, CT appearance of pulmonary nodules, and survival of the two groups were compared.ResultsStage II to IV breast tumors (p < 0.001), high pathological grade of breast cancer (p = 0.001), low proportion of luminal‐type breast cancer (p = 0.003), and the higher serum CYFRA 21‐1 level (p = 0.046) were the clinical characteristics of pulmonary nodules suggestive of POM rather than PLC. The CT features of lung nodules indicative of PLC rather than POM were the subsolid component (p < 0.001), lobulation (p = 0.010), air bronchogram (p < 0.001) and pleural indentation (p = 0.004). Ten‐year survival rate for PLC was 93.2%, which was higher compared with 57.8% in those with POM (p = 0.001).ConclusionsElevated serum CYFRA 21‐1 levels and late‐stage breast cancer may be beneficial for the diagnosis of POM. CT imaging appearances of the subsolid component, lobulation, air bronchogram, and pleural indentation increase the likelihood of PLC. Breast cancer patients with PLC presented better survival with attentive monitoring than those with POM.
Prospective validity evidence for the abbreviated emergency medicine Copenhagen Burnout Inventory
Authors: Reisdorff Earl J. , Johnston Mary M. , Lall Michelle D. , Lu Dave W. , Bilimoria Karl Y. , Barton Melissa A. 
Published: March 2024

AbstractBackgroundPrior research has provided retrospective validity evidence for an abbreviated Copenhagen Burnout Inventory (CBI) to measure burnout among emergency medicine (EM) residents. We sought to provide additional validity and reliability evidence for the two‐factor, six‐item abbreviated CBI.MethodsThis cross‐sectional study used data from the abbreviated CBI that was administered following the 2022 American Board of Emergency Medicine In‐training Examination. Confirmatory factor analysis (CFA) was performed and the prevalence of burnout among EM residents was determined.ResultsOf the 8918 eligible residents, 7465 (83.7%) completed the abbreviated CBI. CFA confirmed the previously developed model of two factors using six items answered with a 1‐ to 5‐point Likert scale. The internal factor was derived from personal and work‐related burnout and the external factor was related to caring for patients. The reliability was determined using Cronbach's alpha (0.87). The overall prevalence of burnout was 49.4%; the lowest prevalence was at the EM1 level (43.1%) and the highest was at the EM2 level (53.8%).ConclusionsCFA of the abbreviated CBI demonstrated good reliability and model fit. The two‐factor, six‐item survey instrument identified an increase in the prevalence of burnout among EM residents that coincided with working in the COVID‐19 environment. The abbreviated CBI has sufficient reliability and validity evidence to encourage its broader use.