bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro‐synthesis of radioleptophos labelled at five different sites
Authors: Zayed S. M. A. D. , Farghaly M. , Fakhr I. M. I. , Hassan A. 
Published: October 2006

AbstractThe micro‐procedure involved interaction of benzene with phosphorus trichloride followed by addition of sulphur. Phenyl dichlorophosphine sulphide was condensed with methanol to form 0‐methyl phenylthiophosphono‐chloridate which was allowed to react with the sodium salt of 4‐bromo‐2,5‐dichlorophenol to yield leptophos [O‐(4‐bromo‐2,5‐dichlorophenyl) 0‐methyl phenyl phosphonothioate]. Five radioactive leptophos compounds were prepared by substituting the cold chemicals with the appropriate radioactive precursor (32PCl3, 1‐14C‐benzene, 35S, 14C‐methanol or tritiated 4‐bromo‐2,5 dichlorophenol).
Preparation of deuterium‐labeled rutin by hydrogen exchange reaction
Authors: Hiraoka K. , Miyamoto T. , Baba S. , Furuta T. 
Published: October 2006

AbstractPreparation of deuterium‐labeled rutin by hydrogen exchange reaction under alkaline condition is described. Hydrogens at positions 2′, 5′ and 6′ of rutin were replaced with deuteriums only on heating, while hydrogens at positions 6 and 8 were readily replaced at room temperature. On the basis of these findings rutin‐2′, 5′, 6′, 6, 8‐d5 was first prepared and then treated with alkaline water at room temperature to obtain rutin labeled with deuteriums at positions 2′, 5′ and 6′.
Synthesis of N‐(4‐pyridyl[14C]carbonylamino)‐1,2,[3H]3,6‐tetrahydropyridine and the mono‐labelled [14C]‐ and [3H]‐analogues
Authors: Mercer J. R. , Wiebe L. I. , Knaus E. E. 
Published: October 2006

AbstractReaction of isonicotinic [14C] acid hydrazide (1) with the Zinke salt (2) afforded N‐(4‐pyridyl [14C] carbonylimino)pyridinium ylide (3) in 81% chemical yield. Sodium borohydride reduction of 3 gave N‐(4‐pyridyl [14C] carbonylamino) −1,2,3,6‐tetrahydropyridine (4) in 60% chemical yield with a specific activity of 6.17 mCi mM−1. Alternatively reduction of N‐(4‐pyridylcarbonylimino) pyridinium ylide (5) using absolute ethanol and [3H]‐water as solvent yielded N‐ (4‐pyridylcarbonylamino)‐1,2,[3H]3,6‐tetrahydropyridine (9) in 52.6% chemical yield with a specific activity of 1.14 mCi mM−1. N‐4‐Pyridyl[14C]carbonylamino‐1,2,[3H]3,6‐ tetrahydropyridine (10) was similarly prepared by sodium borohydride reduction of 3 using absolute ethanol and [3H]‐water as solvent in 46% chemical yield with specific activities of 1.31 and 5.66 mCi mM−1 respectively for [3H] and [14C].
Preparation of 2,3,5,6‐tetrachloropyridine‐2,6‐14C
Authors: McKendry L. H. 
Published: October 2006

AbstractAn 88.6% yield of 2,3,5,6‐tetrachloropyridine‐2,6‐14C (8.99 mCi, 14.5 mCi/mmole) was obtained via chlorination of 2,6‐dichloropyridine‐2,6‐14C.
Synthesis of deuterated and tritiated derivatives of enflurane
Authors: Burke Terrence R. , Pohl Lance R. 
Published: October 2006

AbstractSpecifically deuterated derivatives of the inhalation anesthetic enflurane [2‐chloro‐1‐(difluoromethoxy)‐1,1,2‐trifluoroethane] have been synthesized by a facile base catalyzed exchange. Tritiated enflurane has also been synthesized by this procedure.
Synthesis of 14C‐labelled 4‐(2‐hydroxy‐3‐isopropylaminopropoxy)‐2‐methyl‐indole (mapindolol)
Authors: Illi V. O. , Heindl J. , Balzer J. , Schwartz W. 
Published: October 2006

AbstractMepindolol, a potent ß‐adrenoceptor blocking agent, was labelled with 14C by two different routes yielding the 2‐[14C]methyl and [2‐14C] products. While the first alternative was abandoned because of low overall yield, the latter route provided mepindolol with a specific activity of 2.06 GBq/mmole (55.8 mCi/mmole) and in a radiochemical yield of 40% starting from Ba14CO3.
Synthesis of 123mTe labeled fatty acids
Authors: Mills Stanley L. , Basmadjian Garo P. , Parker George R. , Ice Rodney D. 
Published: October 2006

AbstractFour 123mTe labeled fatty acid analogues were synthesized: 17‐tellura‐9‐octadecenoic acid, 17‐tellura‐9‐nonadecenoic acid, 18‐methyl‐17‐tellura‐9‐nonadecenoic acid and 3‐tellura‐non‐adecanoic acid.Tellurium‐123m metal was solubilized in water with NaBH4. The inorganic nucleophiles produced, NaHTe or Na2Te2 were reacted with 16‐bromo‐9‐hexadecenoic acid in a one or two step procedure to produce the sodium alkyl telluride. To this was added either methyl iodide, ethyl bromide or isopropyl bromide and the resulting 17‐tellura‐fatty acids isolated and formulated. Tellurium‐123m labeled 3‐tellura‐nonadecanoic acid was synthesized via the dihexadecanyl ditelluride or the sodium hexadecanyl telluride route.
Synthese De Phenyl‐1 Propenols Specifiquement Deuteries
Authors: Denhez Jean‐Pierre , Girault Gisèle , Dizabo Pierre 
Published: October 2006

AbstractThe synthesis of deuterated 1‐phenylpropénols is reported. The methods involve either Grignard reactions or selective reduction of α, β insaturated ketones. These ketones are obtained by degradation of ammonium salts of the Mannich bases.
The synthesis of [2‐3H2] taurine and [2‐3H2] hypotarine
Authors: Fellman J. H. 
Published: October 2006

AbstractThe synthesis of [2‐3H2]‐2‐aminoethanesulfonate [2‐3H]‐taurine by the reduction of cyanomethanesulfonic acid with tritium gas is described. The conversion of [2‐3H]‐taurine and its 14C and 35S isotopic forms to 2‐aminoethanesulfinate (hypotaurine) was accomplished by converting taurine to its corresponding sulfonyl chloride and reducing the latter with metallic zinc.
A convenient synthesis of NG‐mono[14C‐methyl]‐L‐arginine
Authors: Abou‐Gharbia M. , Paik W. K. , Swern D. 
Published: October 2006

AbstractNG‐Mono[14C‐methyl]‐L‐arginine was prepared in a two‐step synthesis. N,S‐dimethylthiopseudouronium iodide [methyl‐14C] was prepared in excellent yield and afforded the labelled amino acid on coupling with L‐ornithine.
Synthesis of [8‐3H]Pentostatin
Authors: Putt Sterling R. , Hartman Jon D. , Showalter H. D. Hollis , Kepler John A. , Taylor George 
Published: October 2006

AbstractStarting from 3‐(2‐deoxy‐β‐D‐erythro‐pentofuranosyl)‐6,7‐dihydroimidazo[4,5‐d][1,3]diazepin‐8(3H)‐one (4), the synthesis of both the R‐and S‐isomers of [8‐3H]pentostatin has been achieved. The separation of the desired R‐isomer in Ca. 24% overall chemical yield from the R, S‐mixture was effected by preparative reverse‐phase chromatography utilizing a C18 stationary support. Crystalline product of high specific activity (227 mCi/mmole) was obtained.
Synthese de l'acide nervonique 14C‐24 (acide cis‐tétracosène‐15 oïque 14C‐24)
Authors: Moriya T. , Pabiot M. , Pichat L. 
Published: October 2006

AbstractMethyl iodide‐14C was condensed with 2‐(1‐lithio‐hept‐1‐yn‐6‐yl) 1,3‐dioxolanne 4 in presence of HMPT and benzene to give 2‐(oct‐2‐yn‐8‐yl‐1‐14C) 1,3‐dioxolanne 5.Catalytic reduction of 5, followed by acid hydrolysis led to pelargonic aldehyde 9‐14C 7. Condensation of this aldehyde 7 with an excess of w‐carbomethoxy‐tetradecylidene), triphenyl phosphorane 11 produced methyl nervonate 24‐ 14C 12. The latter was isolated from its trans isomer by chromatography on a silver nitrate impregnated silicagel column. Finally, nervonic acid 24‐14C 13 was obtained by alkaline hydrolysis (specific activity : 52 mCi/mMole) with an overall yield of 7% based on methyl iodide‐14C.
Syntheses of astato compounds by melt method
Authors: Shiue C.‐Y. , Meyer G.‐J. , Ruth T. J. , Wolf A. P. 
Published: October 2006

Abstractm‐Astatobenzoic acid (2) methyl m‐astatobenzoate (3), and m‐astatohippuric acid (4) were synthesized by the melt method. Reaction of methyl m‐bromobenzoate with astatide at 200°C gave methyl m‐astatobenzoate. Compounds 2 and 4 were synthesized by similar method. Compounds 2 and 3 were purified and identified by radio‐gaschromatography.
The synthesis of 13C labelled N, N′‐dimethyl 2‐naphthaleneethanimidamide monohydrochloride, a potential psychotherapeutic agent
Authors: Goralski Christian T. , McCarthy James R. , Linowski J. W. , Nyquist R. A. , Putzig C. L. 
Published: October 2006

AbstractN, N′ ‐Dimethyl 2‐naphthaleneethanimidamide monohydrochloride (DL‐588) is a potential psychotherapeutic agent for which a sample of carbon‐13 labelled material was required for metabolism studies. The sample of 90% 13C enriched DL‐588 was prepared starting with 90% 13C enriched potassium cyanide in three steps. 2‐(Bromomethyl)naphthalene was treated with the 13C potassium cyanide under phase transfer catalysis. The resulting 13C‐2‐napthylacetonitrile was converted to the imidate ester hydrochloride under Pinner reaction conditions (1). This intermediate was treated with excess methylamine in ethanol at 55° to give the title compound in an overall yield of 68%. The effects of 13C labelling on the 1H NMR spectra of the final product and intermediates are discussed.
A convenient synthesis of [carbonyl‐14C]‐4‐hydroxybenzaldehyde
Authors: Holland Herbert L. , Sultana Nighat 
Published: October 2006

AbstractA two‐step synthesis of the title compound from [cyano‐14C]‐copper(I) cyanide is described. The method is extensible to the preparation of carbonyl‐labelled 3,4‐dihydroxybenzaldehyde.
Preparation of all trans‐retinoic‐11‐3H acid and all trans‐retinyl‐11‐3H acetate
Authors: Kaegi Hans H. , Degraw Joseph I. 
Published: October 2006

AbstractTrans‐ionylidineacetaldehyde‐1‐3H (3) was obtained by reduction of unlabeled aldehyde (1) with lithium borotritide, followed by reoxidation of the tritio alcohol (2). Condensation of aldehyde (3) with triethyl 3‐methyl‐4‐phosphonocrotonate (7), followed by saponification of the retinoic ethyl ester (8), afforded trans‐retinoic‐11‐3H acid (9). Esterification of the crystalline acid with diazomethane, and subsequent reduction of the methyl trans‐retinoate‐11‐3H with lithium aluminum hydride, yielded trans‐retinol‐11‐3H, which was acetylated in situ to give trans‐retinyl‐11‐3H acetate (10).
Synthesis of 1‐(3‐trideuteromethoxy‐4‐hydroxyphenyl)‐[1,2,2‐2H3]‐ethyleneglycol ([2H6]MHPG)
Authors: Murray S , Baillie T A , Davies D S 
Published: October 2006

AbstractThe four stage synthesis of a hexadeuterated analogue of the noradrenaline metabolite 3‐methoxy‐4‐hydroxyphenylethylene glycol (MHPG) is described. The overall yield from 3‐trideuteromethoxy‐4‐hydroxyacetophenone is 29%.
Thitium nuclear magnetic resonance spectroscopy. Part 13 [ref. (1)]. Tritium labelled neurochemicals
Authors: Bloxsidge James P. , Elvidge John A. , Gower Marion , Jones John R. , Evans E. Anthony , Kitcher J. Philip , Warrell David C. 
Published: October 2006

AbstractA series of 50 neurochemicals, important for the investigation of neurotransmission processes, has been labelled with tritium, using in all 8 methods. Tritium nuclear magnetic resonance spectroscopy has been employed to determine the positions and extent of labelling in these neurochemicals which can be important for their use in tracer studies. The specificity of the labelling processes is discussed.
Synthesis, analysis, and stability studies of 14C‐methyl(acetoxymethyl)nitrosamine
Authors: Roller Peter P. , Keefer Larry K. , Iii Wallace W. Bradford , Reist Elmer J. 
Published: October 2006

AbstractThe one‐step synthesis of 14C‐methyl(acetoxymethyl)nitrosamine (14C‐DMN‐OAc) of high specific activity is described. The product was shown to decompose by hydrolysis, as reported previously for the unlabelled material, and by self‐radiolysis, a process which occurred even in methylene chloride solution at high specific activities. Both modes of decomposition led to disappearance of label from the solution, at least partly by sorption to the glass storage vessel. Thin‐layer chromatography proved less suitable for resolving the decomposition products than radio‐gas chromatography. Labelled carbon atoms were found to the extent of only 1% in the formaldehyde produced on esterase hydrolysis of 14C‐DMN‐OAc, showing that scrambling during the synthesis was not extensive. Storage of aqueous solutions for biological work was best accomplished in frozen aqueous solutions of pH 5.5.
Chloramine‐t for “no‐carrier‐added” labelling of aromatic biomolecules with bromine‐75,77
Authors: Petzold G. , Coenen H. H. 
Published: October 2006

AbstractThe applicability of chloramine‐T (CAT) was examined for “no‐carrier‐added” labelling of aromatic compounds with the short‐lived isotopes bromine‐77 (T1/2 = 56 h) and bromine‐75 (T1/2 = 98 min). In aqueous solution the optimum reaction conditions with respect to pH, concentration of CAT, reaction time and added brominecarrier were determined using tyrosine as a model substrate. Highest radiochemical yields (>85%) were obtained at pH 1 within a few seconds at a CAT concentration of 10−3 M. At pH 7 a maximum radiochemical yield of 65% was achieved with 10−2 M after 2.5 minutes. Studies with some model peptides showed that only the tyrosyl residues were brominated and similar yields were obtained as in free tyrosine. Some aromatic molecules such as α‐methyl‐tyrosine, phenylalanine, uracil, cytosine, and phenol were radiobrominated by the CAT method with yields up to 90%. Separation and identification of the labelled products were achieved by means of radio high performance liquid chromatography.
Synthesis of 3H‐lorcainide monohydrochloride (R 15 889)
Authors: Thijssen J. B. A. , Knaeps A. G. , Heykants J. J. P. 
Published: October 2006

AbstractLorcainide monohydrochloride, N‐(4‐chlorophenyl)‐N‐/1‐(2‐methylethyl)‐4‐piperidinyl/benzeneacetamide monohydrochioride, is a new orally active antiarrhythmic drug.Incorporation of tritium was achieved by reduction of 4‐chloro‐N‐/1‐(1‐methylethyl)‐4‐piperidinylidene/benzenamine with tritiated sodium borohydride to N‐(4‐chlorophenyl‐1‐1‐methylethyl)‐4‐piperidinamine. This compound was converted in situ into the amide derivative.The radioactive yield of the last two synthesis steps was 82.7 % spread over two fractions with specific activities of 1.1 Ci/mmol and 0.046 Ci/mmol. The labelled compounds were radiochemically pure according to thin‐layer chromatography in three solvent systems, and high‐performance liquid chromatography. The radiochemical stability of lorcainide monohydrochloride investigated in both acidic and alkaline media for 1 hour at 60°C was found to be excellent.
14C‐markierte kohlenwasserstoffe durch katalytische isomerisierung
Authors: Dermietzel Jürgen , Jockisch Wolfgang , Räuber Günther 
Published: October 2006

AbstractA general procedure for the synthesis of labelled hydrocarbons by catalytic isomerization in a microreactor is described.At four studied reactions (synthesis of isohexanes, methylcyclopentane, methylcyclopentenes and xylenes) the thermodynamically expected equilibrium concentrations could be approached. For product separation a direct coupling of the microreactor with a prep‐scale gaschromatographic unit is favorable.
Bledta II: Synthesis of a new tumor‐visualizing derivative of CO(III)‐bleomycin
Authors: Deriemer Leslie H. , Meares Claude F. , Goodwin David A. , Diamanti Carol I. 
Published: October 2006

AbstractA new bifunctional chelating agent, (S)‐l‐(p‐bromoacetamidobenzyl) ethylenedinitrilotetraacetic acid, has been prepared from L‐phenylalanine. Reaction of the chelating agent with the Co(III) complex of the antitumor antibiotic bleomycin‐A2DM was used to generate a new derivative of Co(III)‐bleomycin, BLEDTA II. The product can be radiolabeled quickly by binding a radioactive metal ion to the EDTA group. The in vivo distribution of 111 In‐BLEDTA II in tumor‐bearing mice was determined and found to be indistinguishable from that of the clinically useful 111 In(III)‐BLEDTA I complex (J. Med. Chem. 22: 1019 (1979)).
Synthesis of 3H‐labelled indicine N‐oxide
Authors: Piper James R. , Kari Prasad , Shealy Y. Fulmer 
Published: October 2006

AbstractIndicine N‐oxide, an antitumor agent possessing an unusual type of potential alkylating capacity, was recently selected for clinical trial. Radioactive‐labelled indicine N‐oxide was sought for studies of its biological properties relating to antitumor activity and toxicity. Available indicine N‐oxide was reduced to indicine, and the alkaloid ester was hydrolyzed to its component compounds, retronecine and (−)‐trachelanthic acid. The radioactive label was then introduced into the hydroxymethyl grouping of retronecine by a previously reported procedure involving oxidation of retronecine to methyl 1,2‐dehydro‐7β‐hydroxy‐8α‐pyrrolizidine‐1‐carboxylate followed by reduction of the methoxycarbonyl grouping with LiAl3H4. Recombination of the alcohol and the carboxylic acid, a heretofore challenging problem in syntheses of pyrrolizidine alkaloid esters, was achieved using an adaptation of the mild esterification procedure recently reported by Hassner and Alexanian. By this method, indicine was obtained following reaction of retronecine, the isopropylidene derivative of (−)‐trachelanthic acid, and N,N′‐dicyclohexylcarbodiimide in the presence of 4‐(dimethylamino)pyridine in toluene. Tritium‐labelled indicine thus formed was then treated with m‐chloroperoxybenzoic acid to give labelled indicine N‐oxide.
Synthesis of 14C‐labelled 1‐(4‐chlorobenzyl)‐3‐methyl‐3‐(2‐hydroxyethyl)‐thiourea
Authors: Esses‐Reiter K. , Reiter J. 
Published: October 2006

Abstract1‐(4‐Chlorobenzyl)‐3‐methyl‐3‐(2‐hydroxyethyl)‐thiourea labelled with 14C at its urea group was synthesised starting from potassium cyanide‐14C, via potassium thiocyanate‐14C, 4‐chlorobenzyl‐thiocyanate‐14C and 4‐chlorobenzyl‐isothiocyanate‐14C. The conditions of the isomerisation of 4‐chlorobenzyl‐thiocyanate‐14C to 4‐chlorobenzyl‐isothiocyanate‐14C were studied in detail.
Enzymatic labelling of carcinoembryonic antigen with iodine‐125
Authors: Siuda Andrzej , Lucka Barbara , Russin Krystyna 
Published: October 2006

AbstractCarcinoembryonic antigen /CEA/ was labelled with iodine‐125 using lactoperoxidase. The radiochemical yield of /125I/CEA was about 25% at the specific activity of about 30μCi/μg /1.1MBq/μg/. The percentage binding of /125I/CEA with specific anti‐CEA serum was determined in lyophilized samples during storage. The obtained /125I/CEA preparations can be utilized in radioimmunoassay kits for at least two months.
Synthesis of clomipramine‐d8
Authors: Chaudhuri Naba K. , Sung Ming‐Sang , Markus Bohdan 
Published: October 2006

AbstractThe synthesis of deuterium labelled clomipramine which is an antidepressant drug is described. Eight deuterium atoms have been incorporated specifically into the nonaromatic part of the molecule — six atoms at 1, 2, and 3 positions of the dimethyl‐ aminopropyl side chain and two atoms at the 10 and 11 positions of the molecule. 1,1,2,2,3,3‐Hexadeutero‐3‐dimethylaminopropyl chloride has been synthesized for this purpose and used for the construction of the labelled side chain.
Synthesis of 1‐[2‐hydroxy‐4‐(3‐sulfo‐1‐propyloxy)‐phenyl]‐ ‐3‐(3‐hydroxy‐4‐methoxyphenyl)‐propan‐1‐one‐1‐14C sodium salt
Authors: Koltai E. , Esses‐Reiter K. , Bánfi D. 
Published: October 2006

Abstract1‐[2‐hydroxy‐4‐(3‐sulfo‐1‐propyloxy)‐phenyl]‐3‐ ‐(3‐hydroxy‐4‐methoxyphenyl)‐propan‐1‐one sodium salt labelled with 14C at its carbonyl group (1) was synthetized starting from sodium acetate‐1‐14C through acetyl chloride‐1‐14C, resacetophenone‐carbonyl‐14C (2), 2‐hydroxy‐4‐(3‐sulfo‐1‐propyloxy)‐acetophenone‐carbonyl‐14C sodium salt (3) and 1‐[2‐hydroxy‐4‐(3‐sulfo‐1‐propyloxy)‐phenyl]‐3(3‐hydroxy‐4‐methoxyphenyl)‐2‐ propen‐1‐one‐1‐14C (4).
Synthesis of 14C‐labelled crotamiton
Authors: Chaudhuri Naba K. , Potdar Sudhakar V. , Ball Thomas J. 
Published: October 2006

AbstractThe synthesis of 14C‐labelled crotamiton, which is a fungicide, an insecticide as well as a scabicide is described. Starting from 2‐bromonitrobenzene and Cu14CN, o‐toluidine, labelled with 14C at the methyl group was prepared by the following sequence of reactions: NO2‐C6H4‐Br → NO2‐C6H4‐14CN → NO2‐C6H4‐14COOH → NO2‐C6H4‐14CH2OH → NO2‐C6H4‐14CH2Br → NH2‐C6H4‐14CH3. Labelled o‐toluidine was then heated with crotonic anhydride to give crotonic acid o‐toluidide which was then ethylated by treatment with sodium hydride and ethyl iodide to obtain labelled crotamiton.
Tritium‐labelling of lipids
Authors: Shevchenko V. P. , Myasoedov N. F. 
Published: October 2006

AbstractTritium‐labelled lipid preparations are obtained by heterogeneous isotopic exchange with gaseous tritium in solution. After appropriate purification, the localization of the label was determined in the main fragments of [3H]‐compounds. The obtained data on specific radioactivity and the pattern of label localization allow an insight into processes taking place on labelling under particular experimental conditions.
Synthesis of O,S‐diethyl 2‐(bromomethyl‐13C)‐2‐methylthiomalonate, a model substrate for methylmalonyl‐co a mutase reactions
Authors: Chung S. K. , Hansen J. B. 
Published: October 2006

AbstractO,S‐Diethyl methylthiomalonate was efficiently condensed with paraformaldehyde‐13C in the presence of sodium methoxide to give 2‐[13C]‐hydroxymethylated product, which was converted to 2‐[13C]‐bromomethyl product by treatment with triphenylphosphine and carbon tetrabromide.
Synthese du phosphate d'isopropylamino‐2 pyrimidine (14C2‐4,6) a tres forte activite specifique
Authors: Pabiot J. M. , Pichat L. , Cen‐Saclay  , Esanu A. , Pommier J. , Laurent J. P. 
Published: October 2006

AbstractSyntnesis of very high specific activity (4,6‐14C2) 2‐isopropylaminopyrimidine phosphate (I.A.P.P.)(1‐14C) Sodium acetate (specific activity : 57 mCi/mMole)‐ was transformed into ethyl (1‐ C) acetate with a 76 % yield. The latter was lithiated with lithiodiisopropylamide. The intermediate ethyl (1‐14C) lithioacetate was carbonated with 14CO2 (specific activity : 57 mCi/mMole) to give monoethyl (1,3‐14C2) malonate which was esterified into diethyl (1,3‐14C2) malonate. The latter was condensed with N‐isopropylguanidne to give 4,6‐dihydroxy 2‐iso‐propylamino (4,6‐14C2) pyrimidine. Treatment with POBr3 gave 4,6‐dibromo 2‐isopropylamino (4,6‐14C2) pyrimidine the hydrogenation of which under controlled conditions gave 2‐isopropylamino (4,6‐14C2) pyrimidine isolated as the phosphate. Specific activity was 114 mCi/mMole, radiochemical purity (TLC and HPLC) was 99 % ‐ overall yield was 7 % based on (14 C) carbon dioxide.
Tritium labelled compounds of high specific activity II. amitriptyline
Authors: Zólyomi G. , Sirokmán F. , Tóth G. , Elekes I. 
Published: October 2006

AbstractTritium labelled amitriptyline (1) of high specific activity was prepared. The tritium was incorporated by catalysed isotope exchange into the precursor dibenzosuberone, which had been previously converted into a cyclic ketal, in order to protect the oxo‐group against reduction. It was demonstrated by degradation that isotope exchange occurred exclusively in specified position.
Synthesis and quality control of 125I‐and 14C‐labelled 5‐iodo‐2′‐deoxycytidine triphosphate
Authors: Linz Ute 
Published: October 2006

AbstractA method is described for a no‐carrier‐added synthesis and purification of doubly‐labelled [5‐125I, U‐14C]‐iododeoxycytidine triphosphate. Iodination of 14C‐dCTP was achieved with a radiochemical yield of 80%. Separation and purification of the reaction product were accomplished by reversed‐phase high performance liquid chromatography (RP‐HPLC).
Enzymatic synthesis of radio‐labeled selenocystine from labeled serine and selenide
Authors: Dilworth Gregory L. 
Published: October 2006

AbstractSelenocysteine can be formed enzymically from O‐acetylserine and sodium selenide by O‐acetylserine sulfhydrylase. The preparation of O‐acetylserine from serine and its conversion to selenocystine are described. These reactions can be used to synthesize [14C], [3H], or [75Se]selenocystine.
Preparation of deuterium and tritium labelled norethynodrel, norethindrone, and 6‐methyleneandrostenedione
Authors: Holland Herbert L. , Brown Frances M. , Tan Liat 
Published: October 2006

AbstractA convenient, one step method for the preparation of deuterium or tritium labelled norethynodrel, norethindrone, and 6‐methyleneandrostenedione is reported which utilizes water as the source of label. The choice of exchange reagent (lithium or sodium hydroxide) is crucial in controlling rearrangement of the chemically labile β, γ unsaturated carbonyl function of norethynodrel.