bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adam19 Deficiency Impacts Pulmonary Function: Human GWAS Follow-up in Mouse
Authors: Li Huiling , House John , Nichols Cody , Gruzdev Artiom , Ward James , Li Jian-Liang , Wyss Annah , Haque Ezazul , Edin Matthew , Elmore Susan , Mahler Beth , Degraff Laura , Shi Min , Zeldin Darryl , London Stephanie 
Published: April 2024

Abstract

Purpose
Over 550 loci have been associated with human pulmonary function in genome-wide association studies (GWAS); however, the causal role of most remains uncertain. Single nucleotide polymorphisms in a disintegrin and metalloprotease domain 19 (ADAM19) are consistently related to pulmonary function in GWAS. Thus, we used a mouse model to investigate the causal link between Adam19 and pulmonary function.
Methods
We created an Adam19 knockout (KO) mouse model and validated the gene targeting using RNA-Seq and RT-qPCR. Contrary to prior publications, the KO was not neonatal lethal. Thus, we phenotyped the Adam19 KO.
Results
KO mice had lower body weight and shorter tibial length than wild type (WT). Dual-energy X-ray Absorptiometry indicated lower soft weight, fat weight, and bone mineral content in KO mice. In lung function analyses using flexiVent, compared to WT, Adam19 KO had decreased baseline respiratory system elastance, minute work of breathing, tissue damping, tissue elastance, and forced expiratory flow at 50% forced vital capacity but higher FEV0.1 and FVC. Adam19 KO had attenuated tissue damping and tissue elastance in response to methacholine following LPS exposure. Adam19 KO also exhibited attenuated neutrophil extravasation into the airway after LPS administration compared to WT. RNA-Seq analysis of KO and WT lungs identified several differentially expressed genes (Cd300lg, Kpna2, and Pttg1) implicated in lung biology and pathogenesis. Gene set enrichment analysis identified negative enrichment for TNF pathways.
Conclusion
Our murine findings support a causal role of ADAM19, implicated in human GWAS, in regulating pulmonary function.
Assessing the Clinical Implications of Ischemic Preconditioning through CAIX Expression in Rectal Cancer Patients.
Authors: FRANDON Julien , CHAVEROUX Cedric , LAVAL Pierre-Alexandre , GABARROU Gabrielle , FERRARO-PEYRET Carole , BERTRAND Martin M 
Published: April 2024

Abstract

Background:
Anastomotic leak is a significant concern following rectal cancer surgery, often attributed to ischemic events during surgery. Preoperative ischemic conditioning through embolization of the inferior mesenteric artery may improve colonic perfusion. However, the cellular mechanisms underlying this ischemic preconditioning remain unclear.
Methods:
This ancillary study aimed to explore the cellular correlation of ischemic preconditioning through carbonic anhydrase IX (CAIX) expression in patients undergoing rectal cancer surgery. Normal colonic tissues from ten patients, five with ischemic preconditioning and five controls, were stained against CAIX. The number of intestinal crypts exhibiting CAIX fixation was quantified and compared between the two groups. The resistive index (RI) at the time of surgery and the complications during the 2-year follow-up were also reported.
Results:
Patients who underwent ischemic preconditioning demonstrated a significantly smaller decrease in RI values (p = .03) and less pronounced CAIX fixation compared to controls, although without statistical significance (p = 0.42). CAIX staining was significantly lower in patients without a significant decrease in RI (p = 0.04). Patients with a significant decrease in RI and marked increase in CAIX exhibited a higher rate of complications and lower rate of digestive continuity restoration.
Conclusion:
This study suggests a potential correlation between CAIX expression and RI drop in patients undergoing rectal cancer surgery, indicating a role for CAIX as a cellular marker of ischemic preconditioning. Ischemic preconditioning by early embolization may reduce CAIX expression, suggesting a protective effect on ischemic tissue.
Clinical trial: NCT03628248, 2021-08-04
A novel single kernel parallel image encryption scheme based on a chaotic map
Authors: Bezerra Joao Inacio Moreira , Camargo Vinícius Valduga de Almeida , Soares Rafael Iankowski , Molter Alexandre , Machado Gustavo 
Published: April 2024

Abstract
The development of communication technologies has increased concerns about data security, increasing the prominence of cryptography. Images are one of the most widely shared data, and chaotic ciphers arouse significant interest from researchers, as traditional ciphers are optimized for text encryption. Chaotic encryption schemes perform well for low-quality images, however, they are not fast enough for real-time encryption of Full Ultra HD images. Hence, a novel cipher to be executed in GPU architectures is proposed, where the encryption procedure consists of a single kernel, making it different from previous chaotic works and also from the AES. This enables our work to achieve a throughput of 130.8 GB/s on a GeForce RTX3070 and 251.6 GB/s on a Tesla V100 GPU, an increase of 37\% compared to the AES, and 43 times higher than the previously high for chaotic ciphers. The cipher's security is also verified regarding multiple forms of attacks.
A Neuro-Fuzzy Risk Prediction Methodology in the Automotive Part Industry
Authors: Chakhrit Ammar , Djelamda Imene , Bougofa Mohammed , Guetarni Islam H.M. , Bouafia Abderraouf , Chennoufi Mohammed 
Published: April 2024

Abstract

Failure mode and effects analysis (FMEA) is a systematic and structured method employed across diverse industries to proactively identify and evaluate potential failure modes. In a traditional FMEA, for all failure modes, three criticality parameters: severity, detection, and frequency, are assessed to evaluate criticality. Nevertheless, it frequently has certain flaws. Therefore, in this work, a fuzzy risk proposed model is used to improve the use of the FMEA methodology. The new model uses a fuzzy inference technique in place of the conventional criticality calculation. Fuzzy logic technique is used where the various factors are given as members of a fuzzy set fuzzified by employing adequate membership functions to evaluate the risk and then ranking failure modes and preferring measures to control the risks of undesired events. The Adaptive Neuro-Fuzzy Inference System (ANFIS) is suggested as a dynamic, intelligently proposed model to improve and validate the results acquired by the fuzzy inference system and effectively predict the criticality evaluation of failure modes. Finally, an automotive parts industry case is presented to show the potential of the suggested model. This analysis offers a different ranking of failure modes and improves the decision-making by providing a “preventive –corrective plan. A comparison with existing approaches is presented to demonstrate the efficiency of the suggested approach.
Development and psychometric evaluation of the physical activity and nutritional behavior questionnaire based on social cognitive theory (PANBQ-SCT) in the middle aged at risk of coronary artery disease
Authors: rad Roghayeh Ezati , Hosseini Zahra , Mohseni Shokrollah , Aghamolaei Teamur , Nikparvar Marzieh , Mohammadi Mohammad 
Published: April 2024

Abstract

Background
Coronary artery disease (CAD) is the most common cardiovascular disease and the main cause of mortality in developed and developing countries. Physical activity and nutrition behaviors are modifiable factors in people at risk of CAD and the related risk factors. There is evidence to suggest that socio-cognitive factors play a crucial role in adopting physical activity and nutritional behaviors. The present study aimed to develop and evaluate the psychometric properties of the physical activity and nutritional behavior Questionnaire based on the social cognitive theory (PANBQ-SCT).
Methods
A questionnaire was developed based on the constructs of Bandura’s Social Cognitive Theory, knowledge, self-efficacy, collective efficiency, outcome expectations, observational learning, normative beliefs, social support, barriers and opportunities, reinforcement and punishment, behavioral skills, behavioral intention and nutritional behaviors. The questionnaire was administered as a two-week test-retest among the middle aged at risk of CAD. Confirmatory factor analysis was run to test the model fit of each scale using multiple indices including chi-square test, comparative-fit index (CFI), goodness-of-fit index (GFI), and the root mean square error of approximation (RMSEA). Reliability indices were also tested (ICC and Cronbach’s alpha).
Results
The reliability and factorial validity of each subscale were substantiated. Fit indices suggested that each model was an adequate-to-exact fit to data (CFI = .928, IFI = .894, GFI = .943, TLI = .969, RMSEA = .046). The internal consistency of the test was good, as Cronbach’s alpha coefficient for the entire scale was 0.913, and for the subscales ranged between 0.768 and 0.899. Also, the ICC ranged between 0.643 and 0.918.
Conclusions
The results substantiated the reliability and factorial validity of the socio-cognitive constructs related to physical activity and nutrition behaviors among the middle aged at risk of CAD. Thus, the suggested scale can be used to identify potential socio-cognitive correlates of middle age physical activity and nutrition behaviors, the mediators and validation of theoretical models based on SCT.
Clinical, laboratory, and hospital factors associated with preoperative complications in Peruvian older adults with hip fracture.
Authors: Aguirre-Milachay Edwin , León-Figueroa Darwin A. , Valladares-Garrido Mario J. 
Published: April 2024

Abstract

Objectives
To determine the clinical, laboratory, and hospital factors associated with preoperative complications in older adults with hip fractures.
Methodology:
Analytical observational retrospective cohort study, whose population was older adults with a diagnosis of hip fracture treated in a hospital in northern Peru, during 2017–2019.
Results
432 patients with a median age of 83 years (RIC: 77–88) were evaluated, with the female gender being the most prevalent (60.9%). The most common comorbidities included cardiovascular disease (68%) and diabetes (17.6%), and multimorbidity was observed in 47.2% of cases. The median number of geriatric syndromes was 2 (RIC: 1–5). The overall mortality rate was 3.2% (1.7–5.3). Analysis with Poisson regression model found a significant association with mental red cross > = 3 points RR = 4.29 (1.44–12.78), glucose on admission RR = 1.01 (1.001–1.014) and minimally significantly female sex RR = 2.36 (0.99–5.62)
Conclusions
The most commonly observed complications were infectious in nature, including pneumonia, sepsis, and urinary tract infections. Both mental red cross > = 3 pt, glucose on admission, and minimally female sex were found to increase the risk of developing pre-surgical complications in older adults with hip fracture. The assessment of preoperative and perioperative complications is of great importance in predicting adverse health outcomes in older adults with hip fractures.
Optimizing Subgraph Retrieval and Matching with an Efficient Indexing Scheme
Authors: He Jiezhong , Chen Yixin , Liu Zhouyang , Li Dongsheng 
Published: April 2024

Abstract

Graph index as an effective data structure is widely applied in subgraph retrieval and matching. It records and compares the frequencies of a set of specific features to detect subgraph containment on the fly, which is the foundation of the filtering techniques for subgraph retrieval and matching. However, due to the NP-hardness of the subgraph counting, current graph indices struggle to be built on large graphs. Even counting the simple path and cycle graphs is NP-hard. We observe that the monotone property of the counting process is crucial for the correctness and precision of the index. Therefore, we introduce an efficient graph indexing scheme by counting the path and cycle features monotonically in relaxed semantics. In addition to the filtering techniques, we propose to reorder the search candidates via our index. Experimental results reveal that our index can be constructed significantly faster than existing methods, by 1-3 orders of magnitude, and can handle graphs that are larger than previous work by 1-3 orders of magnitude. Our index-boosted filtering and ordering techniques are proven to be effective in optimizing the subgraph retrieval and matching process.
Construction of a Risk Prediction Model for Pre-eclampsia in Northern China: A Study of 798 Hospitalized Pregnant Women
Authors: Chen Ying , Li Jiaying , Ma Lingyu , Wu Shuyao , Lin Xichen , Yao Yujing , Fan Yiou 
Published: April 2024

Abstract

Objective
To screen the risk factors for pre-eclampsia in Northern China and construct a risk prediction model.
Methods
Clinical data of 798 hospitalized pregnant women from March 2023 to August 2023 at the First Hospital of Jilin University were collected and analyzed. The patients were divided into a pre-eclampsia group (N = 176, case group) and a non-pre-eclampsia group (622 cases, control group) based on clinical diagnosis. Patient medical history, family history, early pregnancy diagnostic data from prenatal care handbooks, and lifestyle information during pregnancy were collected, resulting in a total of 106 parameters for analysis. Univariate analysis and logistic regression analysis were used to identify independent risk factors associated with pre-eclampsia. Subsequently, ROC curve analysis was conducted to evaluate the predictive performance of the model, and a disease prediction model was constructed.
Results
(1) Independent risk factors for pre-eclampsia during early pregnancy included a history of pre-eclampsia, chronic hypertension, family history of hypertension, systolic blood pressure ≥ 120mmHg, diastolic blood pressure ≥ 80mmHg, education level, daily intake of dairy products > 100mL, education level of college or above, and white blood cell count > 1.3×109/L. (2) The probability (P) of prediction was calculated based on these factors using the formula P = 1/[1 + EXP(-1.670 + 3.326×history of pre-eclampsia + 3.151×history of chronic hypertension + 1.276×family history of hypertension + 0.786×systolic blood pressure ≥ 120mmHg + 3.205×diastolic blood pressure ≥ 80mmHg + 0.625×daily intake of dairy products > 100mL-0.792×education level of college or above + 1.000×white blood cell count > 1.3×109/L)]. (3) The area under the ROC curve based on this data was 0.804 [95% CI (0.756, 0.852)], P<0.05. (4) Validation of the model among 107 pregnant women, yielded an accuracy rate of 82.24%.
Conclusion
The risk prediction model, developed using identified risk factors, effectively predicts pre-eclampsia risk in high-risk individuals, offering valuable guidance for clinicians' decision-making.
Association between serum vitamin C and depression in adults: A cross-sectional study of NHANES from 2017 to 2018
Authors: Zhao Dehua , Long Xiaoqing , Wang Jisheng 
Published: April 2024

Abstract

Background
The objective of this cross-sectional study was to explore the association between serum vitamin C and depression in adults.
Methods
Data from the 2017–2018 National Health and Nutrition Examination Survey (NHANES) cycle were utilized. Multivariable logistic regressions were applied to assess the association between serum vitamin C and depression. Restricted cubic spline (RCS) regression was conducted to explore the dose-response relationship between serum vitamin C and depression. In addition, we performed stratified and sensitivity analyses to evaluate the stability of the results.
Results
A total of 4517 participants were included in this study. Among of them, 410 (9.08%) participants suffered depression. In fully adjusted models, serum vitamin C, as a continuous variable, was negatively associated with depression (OR = 0.69, 95%CI: 0.54–0.88, P = 0.003). Similar results were observed when serum vitamin C was analyzed as a categorical variable. The ORs (95% CI) for Q2, Q3, and Q4, with Q1 as the reference, were 1.00 (0.76–1.33), 0.73 (0.54–0.99), and 0.59 (0.42–0.81), respectively. The RCS analysis found a linear negative relationship between serum vitamin C and depression (P for non-linearity = 0.579). Additionally, the inverse associations between serum vitamin C and depression remained robust in stratified and sensitivity analyses.
Conclusion
Our findings suggested that there was a linear negative relationship between serum vitamin C and the risk of depression, indicating that maintaining sufficient serum vitamin C are recommended due to its potential benefits for protecting against depression.
Detection of human enteric viral genes in a non-native winter crane fly, Trichocera maculipennis (Diptera) in the sewage treatment facilities in Antarctic stations
Authors: Lee Sook-Young , Kim Ji Hee , Kang Seunghyun , Park Kye Chung , Cho Sung Mi , Salinas Carla Ximena , Rebolledo Lorena , Benítez Hugo A. , Mejías Tamara Contador , Soutullo Alvaro , Juri Eduardo , Kim Sanghee 
Published: April 2024

Abstract

Background
The Antarctic environment is susceptible to the introduction of non-native species due to its unique ecosystem, which has evolved under geographical isolation and extreme climatic conditions over an extended period. The non-native winter crane fly, Trichocera maculipennis, which was recently introduced to maritime Antarctica, is likely to pose a serious threat to the Antarctic ecosystem. In this study, the possibility of the mechanical transmission of viruses was evaluated.
Methods
The possibility of the mechanical transmission of viruses was evaluated using next-generation sequencing (NGS), quantitative polymerase chain reaction (qPCR), and virus isolation methods from T. maculipennis (Tm)-related samples (Tm body-wash fluid and Tm homogenate) collected from habitats and sewage treatment facilities located at three research stations in Antarctica.
Results
Our findings revealed the presence of human adenovirus (AdV) and human endogenous retrovirus (HERV) in Tm-related samples through virome analysis. Notably, these viruses are commonly detected in human feces. In addition, we identified pepper mild mottle virus (PMMoV) and cucumber green motortle mosaic virus (CGMMV), which are known indicators of enteric viruses, in all Tm-related samples. It is postulated that these viruses originated from wastewater, as evidenced by their detection in the wastewater samples. However, the minute quantities of AdV and HERV genomes detected in Tm-related samples through qPCR, coupled with the observed non-viability of AdV, indicate that T. maculipennis has limited potential for mechanical transmission.
Conclusions
Our study represents the first evaluation of the potential risk of non-native species serving as vectors for pathogens in Antarctica despite the relatively low quantities of detected viruses. This study provides valuable insights for further risk assessments of non-native species that are newly introduced or are likely to be introduced to Antarctica due to climate change or increased human activity.
PTK2 promotes lung cancer progression via cross-talk regulation between EGFR- and TLR-mediated signaling
Authors: Kim Ji Young , Shin Ji Hye , Kim Mi-Jeong , Choi Bongkum , Kang Yeeun , Choi Jimin , Kim Seo Hyun , Kwan Dohee , Kim Duk-Hwan , Chun Eunyoung , Lee Ki-Young 
Published: April 2024

Abstract
Introduction
Protein tyrosine kinase 2 (PTK2) plays a pivotal role in various cancers via cross-talk with growth factor signaling pathways. PTK2 is amplified in non-small cell lung cancer (NSCLC). However, the functional role of PTK2 has not been elucidated yet. Here, we report that PTK2 is functionally implicated in epidermal growth factor receptor (EGFR)- and toll-like receptors (TLRs)-mediated signaling for progression of lung cancer.
Methods
Microarray data of NSCLC tumor tissues and matched normal tissues of 42 NSCLC patients were used to gain insights into associations of PTK2 and EGFR expression with patient’s prognosis and cancer progression. CRISPR-Cas9 gene editing method and cancer progression assay were utilized for functional validation of PTK2 in human A549 and H1299 lung cancer cells. In vitro and in vivo tumorigenic assays were performed using a three-dimensional (3D) tumor spheroid formation and a xenografted NOD scid gamma mouse (NSG, NOD/SCID/IL-2Rγnull) model, respectively.
Results
Patients with up-regulated PTK2 exhibited a poor prognosis after clinical treatments. Gene set enrichment assay (GSEA) revealed that patients with up-regulated PTK2 exhibited high enrichments of gene sets related to lung cancer progression and EGFR- or TLRs-mediated signaling. The functional association between PTK2 and EGFR or TLRs was verified. PTK2-knockout (KO) lung cancer cells exhibited marked attenuations of cancer progression, and in vivo tumorigenic and metastatic activity in xenografted NSG mice. In response to TLR agonists, EGF, or TLR agonists plus EGF, the severe decreases of 3D-tumor spheroid formation could be observed in PTK2-KO lung cancer cells. We further elucidated the molecular mechanism by which PTK2 regulated the cross-talk between EGFR- and TLRs-mediated signaling. PTK2 specifically regulated their downstream molecules for the activation of NF-κB.
Conclusions
Up-regulated PTK2 might be a reliable marker for EGFR- or TLRs-induced lung cancer progression in NSCLC patients. It could be potentially considered as a therapeutic target in the field of precision or personalized cancer medicine aiming for NSCLC intervention.
Tertiary lymphoid structures correlate with better prognosis in patients with retroperitoneal sarcoma: A retrospective study
Authors: Kijima Toshiki , Takada-Owada Atsuko , Kokubun Hidetoshi , Uematsu Toshitaka , Takei Kohei , Betsunoh Hironori , Yashi Masahiro , Ishida Kazuyuki , Kamai Takao 
Published: April 2024

Abstract

B cells are recognized as components of the tumor immune microenvironment in various cancer types, including soft-tissue sarcomas. Recent studies on sarcomas have suggested that tertiary lymphoid structures (TLSs) are associated with B cell expression and immunotherapy efficacy. We evaluated the relationship between the presence of a TLS at the primary site and the prognosis of patients with retroperitoneal sarcoma. TLSs were observed in 17 of 29 patients (59 %). TLSs were observed in 2 of 3 patients (67 %) with well-differentiated liposarcoma, 14 of 16 patients (88 %) with dedifferentiated liposarcoma, and 1 of 2 patients (50 %) with undifferentiated pleomorphic sarcoma. The number of tumor-infiltrating CD8+ T cells was higher in the TLS-positive group than in the TLS-negative group (p = 0.01). Disease-specific survival was significantly longer in patients with TLS-positive tumors than in those with TLS-negative tumors. When patients were divided into groups according to the density of TLSs, the disease-specific curve showed the highest survival in the TLS ≥10 (/100 mm2) group and the lowest survival in the TLS-negative group. When the analysis focused on patients with dedifferentiated liposarcoma, survival was classified according to the density, as in the overall analysis. Negative TLS, positive resection margins, and a lack of systemic therapy at recurrence were independent prognostic predictors. One patients with TLS-positive undifferentiated pleomorphic sarcoma had a microsatellite-unstable tumor and long-term survival with complete disappearance of all metastatic lesions with pembrolizumab, suggesting a prognostic role of TLSs and their predictive role in immunotherapy efficacy.