bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of MTHFR Genotype on Contingent Negative Variation And MRI Abnormalities in Migraine
Authors: De Tommaso Marina , Difruscolo Olimpia , Sardaro Michele , Losito Luciana , Serpino Claudia , Pietrapertosa Anna , Santeramo Maria Teresa , Dicuonzo Franca , Carella Aristide , Lamberti Paolo , Livrea Paolo 
Published: February 2007

Background.—The MTHFR C677T genotype has been associated with increased risk of migraine, particularly of migraine with aura (MA) in selected clinical samples and with elevated homocysteine. The hyper‐homocysteinemia may favor the vascular and neuronal mechanism underlying migraine, and the risk of stroke.Objective.—The first aim of the present study was to examine the Contingent Negative Variation (CNV) amplitude and habituation pattern in a migraine sample versus non‐migraine subjects, at the light of the MTHFR genotype, according to an unrelated and clinical based case‐control panel. The second aim was to compare the frequency of Magnetic Resonance Imaging (MRI) subclinical brain lesions across the different C677 genotypes in the same migraine sample, selected for the young age and the absence of any cardiovascular risk factor.Methods.—One hundred and five 18‐45 year old out‐patients, 90 affected by migraine without aura (MO) and 15 by MA, and 97 non‐migraine healthy subjects, age and sex matched, were selected for the genetic analysis. All subjects had a common ethnic origin from Puglia. Sixty‐four migraine subjects and 33 control subjects were submitted to the recording of the CNV. All migraine subjects underwent the MRI evaluation.Results.—The frequency of homozygosis was 14.33% in normal subjects, versus 25.7% in MA + MO group (χ2‐test: 10.80 P= .001). The frequency of homozygosis in MO patients, was 25.5% (MA versus N: χ2‐test: 9 P= .003), in MA group it was 26.6%.Considering the MTHFR genotype in migraine patients and controls, the C677TT subjects exhibited a reduced habituation index of the early CNV (iCNV), in respect with both C677TC and C677CC; in the migraine group, there was a significant decrease of CNV habituation in patients with homozygosis and a positive correlation between the habituation index values and the homocysteine levels. Nineteen migraine patients exhibited subclinical brain lesions (18.05%): patients with C677T homozygosis did not exhibit a higher risk for MRI abnormalities.Conclusions.—This unrelated and clinical based case‐control study showed that genetically induced hyper‐homocysteinemia may favor the neuronal factors predisposing to migraine, while it does not influence the presence of subclinical vascular brain lesions probably linked with increased risk of stroke.
Headaches Amongst Pool Players
Authors: Seet Raymond C.S. , Chan Yiong Huak , Lim Erle C.H. 
Published: February 2007

Background.—Pool‐players' headache is a newly described entity. We studied the prevalence and risk factors for the development of headaches amongst pool players.Methods.—We obtained demographic information, history of pre‐existing headaches, frequency and duration of pool‐playing, history of pre‐existing headaches and their subtypes, competitive pool‐playing and worsening or development of headaches amongst pool‐players at 2 pool centers. Univariate and multivariate analyses were performed and statistical significance was set at P < .05.Results.—Two hundred and three players, of mean age 22.6 (SD 7.7 years), participated in this study. Of the 21 respondents who reported headaches when playing pool, 11 (52.4%) had pre‐existing headaches and 10 (47.6%) had no pre‐existing headaches. Age and history of pre‐existing headaches were significantly associated with worsening of headaches.Conclusion.—Pool‐players are susceptible to the development of headaches. Avoidance and reduction in the frequency of pool‐playing may reduce the frequency of headaches amongst pool players.
The anti‐angiogenic substance TNP‐470 impairs peri‐implant bone formation: a pilot study in the rabbit metaphysis model
Authors: Mair Birgit , Fuerst Gabor , Kubitzky Pawel , Tangl Stefan , Bergmeister Helga , Losert Udo , Watzek Georg , Gruber Reinhard 
Published: February 2007

AbstractObjectives: To study the osseointegration of dental implants under the conditions of impaired blood vessel formation in a rabbit model.Material and methods: Twenty‐four titanium implants were placed in the diaphyses of 12 rabbits, two in each tibia. After 2 days, six animals received TNP‐470, an anti‐angiogenic substance, 10 mg/kg body weight, three times a week, by subcutaneous injection. The remaining six animals served as controls. All animals were sacrificed 6 weeks post implantation.Results: The amount of newly formed bone (NFB) within a peri‐implant distance of 0.2 mm and the percentage of bone‐to‐implant contacts (BIC) were determined in undecalcified ground sections. In both groups, appositional bone growth originating from the cortex was associated with a dense network of blood vessels. Within 1 mm apical from the implant shoulder, NFB was 29.3±6.7% in TNP‐470 treated animals vs. 44.2±13.1% in the controls (P=0.03). In the adjacent cortical area NFB was 46.1±10.0% in TNP‐470 treated animals and 58.4±3.0% in the control group (P=0.02). In the endosteal area, 3 mm from the implant shoulder NFB was 24.8±11.8% in TNP‐470‐treated animals and 27.0±9.6% in controls (P=0.73) and in the most apical peri‐implant area corresponding to the bone marrow cavity 4.3±3.2% in TNP‐470‐treated animals and 10.5±5.2% in the controls (P=0.03). No decrease in BIC in response to TNP‐470 was observed.Conclusion: The data suggest that TNP‐470 decreases the formation of peri‐implant bone, whereas BIC overall are not affected by it. The rabbit model presented here supports the importance of angiogenesis in the process of peri‐implant bone formation.
Effects of a novel calcium titanate coating on the osseointegration of blasted endosseous implants in rabbit tibiae
Authors: Suh Jo‐Young , Jeung Oh‐Cheol , Choi Byung‐Ju , Park Jin‐Woo 
Published: February 2007

AbstractObjective: The purpose of this study was to investigate the effects of a nanostructured calcium coating on the surfaces of blasted Ti implants on peri‐implant bone formation in the rabbit tibiae.Material and methods: Threaded implants (3.75 mm in diameter, 6 mm in length) were roughened by hydroxyapatite (HA) blasting (control; blasted implants). The implants were then hydrothermally treated in a Ca‐containing solution for 24 h to prepare Ca‐incorporated Ti surfaces (experimental; blasted/Ca implants). Surface characterizations were performed by scanning electron microscopy and stylus profilometry before and after Ca coating. Forty‐two implants (21 control and 21 experimental) were placed in the proximal tibiae of seven New Zealand White rabbits. Each rabbit received six implants. To evaluate the effects of the nanostructured Ca coating on the peri‐implant bone‐healing response, removal torque tests and histomorphometric analyses were performed 6 weeks after surgery.Results: The Ca coating did not significantly change the surface properties produced by blasting at the micron level. Histologically, active bone apposition was observed in the blasted/Ca implants in the marrow space. Compared with the blasted implants, the blasted/Ca implants showed significantly increased bone‐to‐implant contact over the total implant length (P<0.01) and greater mean removal torque values (P<0.05).Discussion and conclusion: The nanostructured, Ca‐incorporated surface significantly enhanced the peri‐implant bone‐healing response of HA‐blasted Ti implants. It may be concluded that the use of nanostructured, Ca‐coated surfaces may have synergic effects in enhancing osseointegration of blasted Ti implants due to their micron‐scaled surface properties and biologically active surface chemistry.
Peri‐implant hard and soft tissue integration to dental implants made of titanium and gold
Authors: Abrahamsson Ingemar , Cardaropoli Giuseppe 
Published: February 2007

AbstractObjectives: The aim of the study is to compare the peri‐implant hard and soft tissue integration around dental implants made of commercially pure (c.p.) titanium or a gold alloy but with the same shape and surface roughness.Material and methods: In four beagle dogs, all mandibular premolars were extracted. Three months later, four experimental non‐submerged implants were placed in each edentulous premolar region. Each implant comprised three different zones: zone A (coronal), zone B (central) and zone C (apical). Each zone was made of either c.p. titanium or a gold alloy. Four different combinations of metal and zone were used. A plaque control program was initiated and 6 months later, the animals were sacrificed and biopsies were obtained. The biopsies including the implant and the surrounding tissues were processed for ground sectioning.Results: The height of the peri‐implant mucosa and the length of the barrier epithelium were similar at the four experimental sites. The marginal bone level in the different metal combinations was located between 4.5 and 4.8 mm apical of the implant rim. The percent of mineralized bone that was in direct contact with the implant surface (BIC%) was consistently greater in the marginal than in the apical portion of the implants. The BIC% for the marginal and apical zone were consistently greater for implant portions made of titanium than for portions made of gold alloy (zone B: 42.7% vs. 36.5%, zone C: 33.2% vs. 19%).Conclusions: Osseointegration was achieved to surfaces made of both c.p. titanium and a gold alloy. BIC% was higher at titanium than at gold surfaces. Moreover, the peri‐implant soft tissue dimensions were not influenced by the metal used in the ‘marginal’ zone of the implant.
Bone healing in critical‐size defects treated with bioactive glass/calcium sulfate: a histologic and histometric study in rat calvaria
Authors: Furlaneto Flávia A. C. , Nagata Maria J. H. , Fucini Stephen E. , Deliberador Tatiana M. , Okamoto Tetuo , Messora Michel R. 
Published: February 2007

AbstractObjective: The purpose of this study was to analyze histologically the influence of bioactive glass (BG) with or without a calcium sulfate (CS) barrier on bone healing in surgically created critical‐size defects (CSD) in rat calvaria.Material and methods: A CSD was made in each calvarium of 48 rats. They were divided into three groups: C (control): blood clot only; BG: defect filled with BG; and BG/CS: defect filled with BG covered by a CS barrier. Animals were euthanized at 4 or 12 weeks. Formation of new bone was evaluated histomorphometrically.Results: No defect completely regenerated with bone. BG particles were observed in Groups BG and BG/CS at both periods of analysis. The thickness throughout the healing area in Groups BG and BG/CS was similar to the original calvarium, while Group C presented a thin connective tissue in the center of the defect in both periods of analysis. At 4 weeks, Groups C and BG/CS presented significantly more bone formation than Group BG. No significant differences were found between Groups C and BG/CS. At 12 weeks, no significant differences in the amount of bone formation were observed among the three groups. When comparing 4 and 12 weeks, there was a significant increase in new bone formation within groups BG and BG/CS, but not C.Conclusion: BG particles, used with or without a CS barrier, maintained the volume and contour of the area grafted in CSD. However, they did not lead to a significant difference in bone formation when compared with control at 12 weeks post‐operative.
In vitroevaluation of different heat‐treated radio frequency magnetron sputtered calcium phosphate coatings
Authors: Yonggang Yan , Wolke Joop G. C. , Yubao Li , Jansen John A. 
Published: February 2007

AbstractObjectives:Surface chemical compositions, such as calcium/phosphorus ratio and phase content, have a strong influence on the bioactivity and biocompatibility of calcium phosphate (CaP) coatings as applied on orthopedic and dental implants.Material and methods:Hydroxylapatite (HA) and dicalcium pyrophosphate (DCPP) coatings were prepared on titanium substrates by RF magnetron sputter deposition. The surfaces were left as‐prepared (amorphous HA coating; A‐HA, amorphous DCPP coating; A‐DCPP) or heat treated with: infrared (IR) at 550°C (I‐HA) or at 650°C (I‐DCPP), and a water steam at 140°C (S‐HA and S‐DCPP). The surface changes of these coatings were determined after incubation in simulated body fluid (SBF). Also, the growth of rat bone marrow cells (RBM) was studied with scanning electron microscopy (SEM).Results:Both IR and water steam heat treatment changed the sputter‐deposited coatings from the amorphous into the crystalline phase. As‐prepared amorphous coatings dissolved partially in SBF within 4 weeks of incubation, while heat‐treated coatings supported the deposition of a precipitate, i.e., carbonated apatite on both I‐HA and S‐HA specimens, and tricalciumphosphate on the I‐DCPP and S‐DCPP specimens. The Ca/P ratio of the A‐HA, I‐HA, S‐HA, A‐DCPP, I‐DCPP and S‐DCPP coatings changed, respectively, from 1.98 to 1.12, 2.01 to 1.76, 1.91 to 1.68, 0.76 to 1.23, 0.76 to 1.26 and 1.62 to 1.55 after 4 weeks of incubation in SBF. Finally, the RBM cells grew well on all heat‐treated coatings, but showed different mineralization morphology during cell culturing.Conclusion:The different heat‐treatment procedures for the sputtered HA and DCPP coatings influenced the surface characteristics of these coatings, whereby a combination of crystallinity and specific phase composition (Ca/P ratio) strongly affected theirin vitrobioactivity.
Effect of Bio‐Oss® on osseointegration of dental implants surrounded by circumferential bone defects of different dimensions: an experimental study in the dog
Authors: Polyzois Ioannis , Renvert Stefan , Bosshardt Dieter D. , Lang Niklaus P. , Claffey Noel 
Published: February 2007

AbstractObjectives: This study was designed to evaluate the effect of gap width and graft placement on bone healing around implants placed in simulated extraction sockets of various widths in four Labrador dogs.Materials and Methods: Five Osseotite® implants per dog were placed in the mandible of four dogs. Two implants were inserted into sites with a 2.37 mm and two with a 1 mm gap present between the implants and bone around the coronal 6 mm of the implants in each dog. For one of each gap sizes, the gap was filled with Bio‐Oss®, and the other two with blood alone. A fifth implant was inserted without a gap and used as a control. Ground sections were prepared from biopsies taken at 4 months and histometric measurements of osseointegration and bone between the threads made for the coronal 6 mm.Results: The medians for osseointegration ranged from 5.2 mm for control to 1–2.6 mm for the test modalities. There were significant differences for linear measurements of osseointegration (χ2 18.27; df 4; P=0.0011) and bone area within threads (χ2 23.4; df 4; P=0.0001) between test modalities.Conclusions: The results suggest that the wider the gap around the implants, the less favourable the histological outcome at short time intervals following treatment. They also infer that bone grafting with anorganic bovine bone xenograft seems to lead to a more favourable histological outcome for wider circumferential defects but not for narrower defects.
Effectiveness of two oral pastes for the treatment of recurrent aphthous stomatitis
Authors: Rodríguez M , Rubio JA , Sanchez R 
Published: February 2007

Objective:  To compare the effectiveness of two topical medications to reduce the pain and size of recurrent minor aphthous ulcers.Setting:  Ten Colombian Dental Faculties’ clinics.Design:  A double‐blind randomized multi‐centre clinical study.Subjects:  Ninety‐six patients complaining of at least five acute aphthous ulcers were randomized to two intervention groups. Sample size was calculated using an alpha error of 0.05 and beta of 0.20Materials and methods:  Participants were randomly assigned to receive 5% amlexanox or a 0.05% clobetasol propionate magistral preparation. Observers at the participating institutions were previously trained to standardize clinical diagnosis and data recollection. Ulcer size and pain were measured on treatment days 0, 2 and 5.Results:  No significant differences were found between the two groups studied in any of the studied variables at baseline. Both treatment medications significantly reduced pain magnitude and the index ulcer's size on days 2 and 5 compared with day 0 without adverse reactions (within groups differences). No statistical differences between groups of the study medications were found.Conclusion:  The two treatments applied had similar effectiveness as they both relieved pain and reduced the size of recurrent aphthous ulcers.
Acute upper airway obstruction from acquired angioedema
Authors: Cheng William YC , Smith William B , Russell W John 
Published: February 2007

AbstractC1 esterase inhibitor deficiency is an unusual cause of acute upper airway angioedema. This case of angioedema is secondary to acquired C1 esterase inhibitor deficiency associated with neoplastic disease and triggered by the use of angiotensin converting enzyme inhibitors. It was sufficiently severe to require emergency airway management. A guide to the evaluation and management of angioedema is presented.
Detection of BRAF V600E activating mutation in papillary thyroid carcinoma using PCR with allele-specific fluorescent probe melting curve analysis
Authors: Rowe Leslie R , Bentz Brandon G , Bentz Joel S 
Published: February 2007

Background:A single hotspot mutation at nucleotide 1799 of theBRAFgene has been identified as the most common genetic event in papillary thyroid carcinoma (PTC), with a prevalence of 29–83%.Aims:To use a PCR assay to molecularly characterise theBRAFactivating point mutation in a series of PTC and benign thyroid cases and correlate the mutation results with histological findings.Methods:Formalin-fixed paraffin-embedded (FFPE) sections were evaluated for theBRAFV600E mutation using LightCycler PCR with allele-specific fluorescent probe melting curve analysis (LCPCR).Results:42 (37 PTC; 5 benign) surgical tissue samples were analysed for theBRAFV600E activating point mutation. Using LCPCR and direct DNA sequencing, theBRAFmutation was identified in 23/37 (62.2%) PTC FFPE samples. DNA sequencing results demonstrated confirmation of the mutation.Conclusions:Detection of BRAF-activating mutations in PTC suggests new approaches to management and treatment of this disease that may prove worthwhile. Identification of the BRAF V600E activating mutation in routine FFPE pathology samples by a rapid laboratory method such as LCPCR could have significant value.
Periacinar retraction clefting in proliferative prostatic atrophy and prostatic adenocarcinoma
Authors: Ulamec Monika , Tomas Davor , Ensinger Christian , Čupić Hrvoje , Belicza Mladen , Mikuz Gregor , Krušlin Božo 
Published: February 2007

Aims:To evaluate the presence and extent of periacinar retraction clefting in proliferative prostatic atrophy and carcinoma in radical prostatectomy specimens.Methods:Atrophic foci and neoplastic glands were analysed in specimens from 50 patients who underwent radical prostatectomy. Analysed atrophic glands were classified in two main groups, proliferative atrophy (PA) and proliferative inflammatory atrophy (PIA); each group was subclassified into simple atrophy (SA) and postatrophic hyperplasia (PAH). According to the presence and extent of periacinar retraction clefting, atrophic and neoplastic glands were classified as: group 1, glands without clefts or with clefts affecting ⩽50% of gland circumference; group 2, glands with clefts that affected >50% of the circumference in <50% of examined glands; and group 3, glands with clefts that affected >50% of the circumference in ⩾50% of examined glands.Results:Forty-four (88.0%) atrophic foci were without periacinar clefts or clefts were present in less than half of the gland circumference (group 1). In 6 (12.0%), atrophic foci clefts affected >50% of gland circumference (groups 2 and 3). Forty-five (90.0%) carcinomas were with clefts which affected more than 50% of gland circumference (groups 2 and 3); and in five carcinomas only, clefts were not found or affected <50% of gland circumference (group 1).Conclusion:Results indicate that periacinar retraction clefting represents a reliable criterion in differential diagnosis between proliferative atrophy and carcinoma.
Blood Levels of Long-Chain Polyunsaturated Fatty Acids, Aspirin, and the Risk of Colorectal Cancer
Authors: Hall Megan N. , Campos Hannia , Li Haojie , Sesso Howard D. , Stampfer Meir J. , Willett Walter C. , Ma Jing 
Published: February 2007

Abstract
Background: N-3 fatty acids may decrease risk of colorectal cancer by inhibiting the cyclooxygenase-2 enzyme and production of proinflammatory eicosanoids derived from arachidonic acid (20:4n-6). Aspirin also inhibits the cyclooxygenase-2 enzyme and may share with n-3 fatty acids a potential mechanism to decrease the risk of colorectal cancer.
Methods: We conducted a nested case-control analysis using blood samples collected from the Physicians' Health Study participants in 1982 to 1984. N-3 and n-6 fatty acid levels were measured using gas-liquid chromatography for 178 men who developed colorectal cancer through December 31, 1995 and 282 age- and smoking-matched controls. We used conditional logistic regression to examine associations. All statistical tests were two-sided.
Results: Total long-chain n-3 fatty acids were nonsignificantly inversely associated with colorectal cancer risk [relative risk (RR) for highest versus lowest quartile, 0.60; 95% confidence interval (95% CI), 0.32 to 1.11; Ptrend = 0.10], after adjustment for possible confounders. We observed potential interaction between randomized aspirin assignment and long-chain n-3 fatty acid levels (Pinteraction = 0.04). Among men not on aspirin, RRs (95% CI) for increasing quartiles of long-chain n-3 fatty acids were 1.00 (reference), 0.60 (0.28-1.28), 0.51 (0.22-1.17), and 0.34 (0.15-0.82), Ptrend = 0.006. For participants taking aspirin, there was no additional benefit of increasing n-3 fatty acid levels. The RR (95% CI) for the highest versus lowest quartile of n-6 fatty acids was 0.64 (0.35-1.17).
Conclusions: Blood levels of long-chain n-3 fatty acids were associated with decreased risk of colorectal cancer among men not using aspirin. N-6 fatty acids were nonsignificantly inversely associated with colorectal cancer risk. (Cancer Epidemiol Biomarkers Prev 2007;16(2):314–21)
In situ Assessment of Intraepithelial Neoplasia in Hamster Trachea Epithelium Using Angle-Resolved Low-Coherence Interferometry
Authors: Chalut Kevin J. , Kresty Laura A. , Pyhtila John W. , Nines Ronald , Baird Maureen , Steele Vernon E. , Wax Adam 
Published: February 2007

Abstract
Optical spectroscopy was used to evaluate the transformation of nuclear morphology associated with intraepithelial neoplasia in an animal model of carcinogenesis. In this pilot study, we have assessed the capability of angle-resolved low-coherence interferometry (a/LCI) to monitor in situ the neoplastic progression of hamster trachea epithelial tissue. By using the depth resolution made possible by coherence gating, the a/LCI system has been adapted to the unique geometry of the hamster trachea to allow us to extract useful nuclear morphometric information from cells in the epithelial layer without the need for exogenous staining or tissue fixation. Analysis of a/LCI nuclear morphology measurements has identified two important biomarkers of neoplastic transformation in hamster trachea epithelium, the size and the refractive index of epithelial cell nuclei. By comparing the a/LCI measurements of these two biomarkers to pathologic classification, we distinguished nuclear morphology changes for normal tissue, low-grade dysplasia, and high-grade dysplasia. Given its previous usefulness for tracking neoplastic change through nuclear morphometry measurements, the a/LCI technique may prove to be a useful tool in evaluating chemopreventive agents in future studies of hamster trachea epithelium. (Cancer Epidemiol Biomarkers Prev 2007;16(2):223–7)