bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of cAMP on the first mitotic cell cycle of mouse embryos
Authors: Yu Aiming , Zhang Zhe , Bi Qiang , Sun Bingqi , Su Wenhui , Guan Yifu , Mu Runqing , Miao Changsheng , Zhang Jie , Yu Bingzhi 
Published: November 2007

AbstractMitosis promoting factor (MPF) plays a central role during the first mitosis of mouse embryo. We demonstrated that MPF activity increased when one‐cell stage mouse embryo initiated G2/M transition following the decrease of cyclic adenosine 3′, 5′‐monophosphate (cAMP) and cAMP‐dependent protein kinase (PKA) activity. When cAMP and PKA activity increases again, MPF activity decreases and mouse embryo starts metaphase–anaphase transition. In the downstream of cAMP/PKA, there are some effectors such as polo‐like kinase 1 (Plk1), Cdc25, Mos (mitogen‐activated protein kinase kinase kinase), MEK (mitogen‐activated protein kinase kinase), mitogen‐activated protein kinase (MAPK), Wee1, anaphase‐promoting complex (APC), and phosphoprotein phosphatase that are involved in the regulation of MPF activity. Here, we demonstrated that following activation of MPF, MAPK activity was steady, whereas Plk1 activity fluctuated during the first cell cycle. Plk1 activity was the highest at metaphase and decreased at metaphase–anaphase transition. Further, we established a mathematical model using Gepasi algorithm and the simulation was in agreement with the experimental data. Above all the evidences, we suggested that cAMP and PKA might be the upstream factors which were included in the regulation of the first cell cycle development of mouse embryo. Mol. Reprod. Dev. 75: 489–495, 2008. © 2007 Wiley‐Liss, Inc.
Fear and pulmonary stress behaviors to an asphyxial threat across cognitive states
Authors: Campbell Margaret L. 
Published: November 2007

AbstractThe purpose of this exploratory study was to identify behaviors that may signify respiratory distress across cognitive states in response to an asphyxial threat. Patients undergoing a ventilator weaning trial were assessed and observed at baseline and during weaning with a capnograph/oximeter and video camera. Cognitive state was categorized at baseline, and an emotion report was elicited after the trial. Pulmonary stress and fear behaviors were similar across cognitive states. Hypercarbia predicted activation of fear behaviors. Gender differences characterized emotion reporting. An asphyxial threat may induce an innate array of behaviors that cannot be volitionally controlled and that may have the same appearance across cognitive states. Recognizing respiratory distress behaviors may improve nursing care of patients who are cognitively impaired. © 2007 Wiley Periodicals, Inc. Res Nurs Health 30:572–583, 2007
Health literacy and health outcomes in HIV seropositive persons
Authors: Nokes Kathleen M. , Coleman Christopher Lance , Cashen Margaret , Dole Pamela , Sefcik Elizabeth , Hamilton Mary Jane , Kirksey Kenn M. , Huang Emily Hai , Apinya Jumpamool , Holzemer William 
Published: November 2007

AbstractInadequate health literacy has been associated with worse health status and more hospitalizations. We examined the influence of personal characteristics and health literacy on body change distress, depressive symptoms, and HIV symptom intensity in a convenience sample of 489 HIV‐seropositive adults throughout the United States. Health literacy was measured with the Rapid Estimate of Adult Literacy in Medicine (REALM) instrument. Those with higher health literacy scores reported more body change distress, depressive symptoms, and HIV symptom intensity. Being Latino and having higher health literacy scores were associated with poorer health outcomes. These results challenge the utility of the REALM to examine the relationship between health literacy and adverse health status. © 2007 Wiley Periodicals, Inc. Res Nurs Health 30:620–627, 2007
“It's not just about MOMMAS”: African‐American non‐resident fathers' views of paternal involvement
Authors: Julion Wrenetha , Gross Deborah , Barclay‐McLaughlin Gina , Fogg Louis 
Published: November 2007

AbstractMany social and economic policies have been developed to increase fathers' involvement with their children. Yet, we know little about the meaning of involvement for African‐American non‐resident fathers. The purpose of this study was to obtain African‐American non‐resident fathers' perspectives on involvement and perceptions of their involvement. Seven focus groups were conducted with 69 fathers. Fathers' views of involvement were grouped into four major areas of importance, including sharing and caring, providing guidance, providing support, and serving in culturally specific roles. Fathers described many impediments to, and expressed dissatisfaction with, their level of involvement. The findings support the need for father‐focused interventions. © 2007 Wiley Periodicals, Inc. Res Nurs Health 30:595–610, 2007
In vitro biocompatibility of three‐dimensional chitosan scaffolds immobilized with chondroitin‐6‐sulfate
Authors: Jou Chi‐Hsiung , Chen Wei‐Chun , Yang Ming‐Chien , Hwang Mou‐Chen , Chou Wen‐Li , Lin Shang‐Ming , Hsu Cheng‐Yi 
Published: November 2007

AbstractCellular‐compatible scaffolds were prepared using a three‐dimensional micro‐porous chitosan (CS) non‐woven fabric immobilized by glutaraldehyde (GA), followed by the immobilization of chondroitin‐6‐sulfate (ChS). To characterize the immobilizing process, tensile analysis, and scanning electron microscopy (SEM) were performed. The cell seeding efficiency and proliferation test were evaluated using L929 fibroblasts. The chitosan scaffolds showed high water vapor transmission rate and antibacterial activity. In addition, ChS‐immobilized scaffolds exhibited higher cell seeding efficiency and fibroblasts proliferation. These results demonstrated that the CS non‐woven fabrics grafted with GA and immobilized with ChS could be an appropriate candidate for wound healing and artificial scaffolds in the clinical applications. Copyright © 2007 John Wiley & Sons, Ltd.
Peripheral T‐cell lymphoma in childhood: Population‐based experience in the United Kingdom over 20 years
Authors: Windsor Rachael , Stiller Charles , Webb David 
Published: November 2007

AbstractBackgroundPeripheral T‐cell lymphomas (PTCL) are very rare in children and this has prevented assessment of best treatment and prognosis.ProcedureRegistry‐based experience in England, Scotland and Wales over a 20‐year period was studied to address these shortfalls. Anaplastic large cell lymphoma and mycosis fungoides were excluded due to recent publications describing UK experience with these disorders.ResultsTwenty‐five cases were identified, comprising 1.6% of non‐Hodgkin lymphoma (NHL) registrations; 17 (68%) children with PTCL‐unspecified (PTCL‐u), 3 (12%) with angiocentric PTCL, 3 (12%) with angioimmunoblastic PTCL, and 2 (8%) with subcutaneous panniculitis‐like T‐cell lymphoma. Eighteen children were male, with a male/female ratio of 2.6:1. Median age was 7 (range 1–14) years. Eleven children (44%) died and actuarial survival was 76% at 1 year, 64% at 3 years and 59% at 5 years. Treatments given were subdivided between group T (regimens for T NHL or acute lymphoblastic leukaemia) or group B (regimens for B NHL). Amongst the 17 children with PTCL‐u, 9/12 children in group T survived compared with 1/5 group B.ConclusionsThe Children's Cancer and Leukaemia Group recommendation that children with PTCL‐u receive group T therapy is supported by this series. The numbers of children with other types of PTCL were too small to allow conclusions on best therapy. Pediatr Blood Cancer 2008;50:784–787. © 2007 Wiley‐Liss, Inc.
Proteome analysis of metastatic colorectal cancer cells recognized by the lectin Helix pomatia agglutinin (HPA)
Authors: Saint‐Guirons Julien , Zeqiraj Elton , Schumacher Udo , Greenwell Pamela , Dwek Miriam 
Published: November 2007

AbstractThe lectin from Helix pomatia (HPA) binds to adenocarcinomas with a metastatic phenotype but the glycoconjugates of cancer cells that bind to the lectin have yet to be characterized in detail. We used a model of metastatic (HT29) and nonmetastatic (SW480) human colorectal cancer cells and a proteomic approach to identify HPA binding glycoproteins. Cell membrane proteins purified by HPA affinity chromatography, were separated by 2‐DE and analyzed by MS. Competitive inhibition experiments with N‐acetylgalactosamine, N‐acetylglucosamine, and sialic acid confirmed that HPA binding was via a glycan‐mediated interaction. Western blot analysis showed that HPA binds to proteins not recognized by an antibody against blood group A epitope. The proteomic study showed the main HPA binding partners include integrin αv/α6 and annexin A2/A4. These proteins were found complexed with microfilament proteins α and β tubulin, actin, and cytokeratins 8 and 18. HPA also bound to Hsp70, Hsp90, TRAP‐1, and tumor rejection factor 1. This study revealed that the prognostic utility of HPA lies in its ability to bind simultaneously to many glycoproteins involved in cell migration and signaling, in addition, the proteins recognized by HPA are glycosylated with structures distinct from the blood group A epitope.
Evidence for a protective response by the gill proteome of rainbow trout exposed to X‐ray induced bystander signals
Authors: Smith Richard W. , Wang Jiaxi , Bucking Carol P. , Mothersill Carmel E. , Seymour Colin B. 
Published: November 2007

AbstractThe bystander effect occurs when cells which are not directly exposed to radiation, but which receive signals from irradiated cells, respond as though they were irradiated. An X‐ray induced bystander effect has been demonstrated in rainbow trout gills. Therefore, a proteomic comparison was made of gills from X‐ray treated trout and trout exposed to X‐ray induced bystander signals. 2‐D gel analysis revealed X‐ray exposure increased the expression of the cancer related protein annexin II. The proteomic changes associated with the bystander effect differed from those associated with direct radiation exposure. Expression of a hemopexin‐like protein, Rho GDP dissociation inhibitor (RhoGDI) and pyruvate dehydrogenase (PDH) were increased. These proteins possess protective properties against reactive oxygen damage (a component of the bystander signal), regulate epithelial polarity and prevent lactate acidosis, respectively. There was also evidence for an increase in chromosome 1 SR‐like CTD‐associated factor (SCAF) protein turnover, which could suggest the protective response is transcriptionally regulated. The freshwater fish gill is a polarised barrier, separating against an external hypotonic environment. Since the maintenance of epithelial polarity is vital to gill function, these bystander effect proteomic changes could collectively protect the structural, functional and intracellular integrity of gill epithelia.
Overexpression of annexin a1 induced by terephthalic acid calculi in rat bladder cancer
Authors: Cui Lunbiao , Wang Yubang , Shi Yuan , Zhang Zhengdong , Xia Yankai , Sun Hong , Wang Shouling , Chen Jianfeng , Zhang Wei , Lu Qiang , Song Ling , Wei Qingyi , Zhang Ruiwen , Wang Xinru 
Published: November 2007

AbstractProlonged cell proliferation in response to irritation by bladder calculi can evoke malignant transformation of the urothelium. However, the molecular mechanisms responsible for calculi‐associated bladder carcinogenesis are unknown. We compared the protein expression pattern of rat bladder transitional cell carcinomas (TCCs) induced by terephthalic acid with that of normal bladder tissues using 2‐DE. Comparative analysis of the respective spot patterns on 2‐DE showed 146 spots that were markedly changed in TCC samples. Subsequently, 56 of the variant protein spots were identified by MALDI‐TOF MS. Among them, overexpression of annexin a1 (ANNA1) in rat TCCs was confirmed by Western blotting and real‐time RT‐PCR analysis. Immunohistochemical staining revealed that ANNA1, usually a cytoplasmic protein in normal urothelium, was translocated to the nucleus in rat bladder cancer cells. In contrast to the animal studies, examination of human clinical specimens showed that ANNA1 expression was reduced in TCC compared to normal urothelium. The expression of ANNA1 was inversely related to the level of differentiation of TCC. Our data suggest that overexpression of ANNA1 is involved in bladder carcinogenesis induced by bladder calculi and that translocation of the protein may be partly responsible for the effect. ANNA1 may serve as a new marker of differentiation for the histopathological grading of human TCC.
A–B–A stereoblock copolymers of N‐isopropylacrylamide
Authors: Nuopponen Markus , Kalliomäki Katriina , Laukkanen Antti , Hietala Sami , Tenhu Heikki 
Published: November 2007

AbstractA–B–A stereoblock polymers with atactic poly(N‐isopropylacrylamide) (PNIPAM) as a hydrophilic block (either A or B) and a non‐water‐soluble block consisting of isotactic PNIPAM were synthesized using reversible addition fragmentation chain transfer (RAFT) polymerizations. Yttrium trifluoromethanesulfonate was used in the tacticity control, and bifunctional S,S′‐bis(α,α′‐dimethyl‐α″‐acetic acid)‐trithiocarbonate (BDAT) was utilized as a RAFT agent. Chain structures of the A–B–A stereoblock copolymers were determined using 1H NMR, SEC, and MALDI‐TOF mass spectrometry. BDAT proved to be an efficient RAFT agent in the controlled synthesis of stereoregular PNIPAM, and both atactic and isotactic PNIPAM were successfully used as macro RAFT agents. The glass transition temperatures (Tg) of the resulting polymers were measured by differential scanning calorimetry. We found that the Tg of isotactic PNIPAM is molecular weight dependent and varies in the present case between 115 and 158 °C. Stereoblock copolymers show only one Tg, indicating the miscibility of the blocks. Correspondingly, the Tg may be varied by varying the mutual lengths of the A and B blocks. The phase separation of aqueous solutions upon increasing temperature is strongly affected by the isotactic blocks. At a fixed concentration (5 mg/mL), an increase of the isotacticity of the stereoblock copolymers decreases the demixing temperature. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 38–46, 2008
Enhancement of photocurrent of polymer‐gelled dye‐sensitized solar cell by incorporation of exfoliated montmorillonite nanoplatelets
Authors: Tu Chi‐Wei , Liu Ken‐Yen , Chien An‐Ting , Yen Ming‐Huei , Weng Ting Hsiang , Ho Kuo‐Chuan , Lin King‐Fu 
Published: November 2007

AbstractPoly(n‐isopropylacrylamide) (PNIPAAm) and its nanocomposite with exfoliated montmorillonite (MMT) were prepared by soap‐free emulsion polymerization and individually applied to gel the electrolyte systems for the dye‐sensitized solar cells (DSSCs). Each exfoliated MMT nanoplatelet had a thickness of ∼ 1 nm, carried ∼ 1.8 cation/nm2, and acted like a two‐dimensional electrolyte. The DSSC with the LiI/I2/tertiary butylpyridine electrolyte system gelled by this polymer nanocomposite had higher short‐circuit current density (Jsc) compared to that gelled by the neat PNIPAAm. The former has a Jsc of 12.6 mA/cm2, an open circuit voltage (Voc) of 0.73 V, and a fill factor (FF) of 0.59, which harvested 5.4% electricity conversion efficiency (η) under AM 1.5 irradiation at 100 mW/cm2, whereas the latter has Jsc = 7.28 mA/cm2, Voc = 0.72 V, FF = 0.60, and η = 3.17%. IPCE of the nanocomposite‐gelled DSSC were also improved. Electrochemical impedance spectroscopy of the DSSCs revealed that the nanocomposite‐gelled electrolytes significantly decreased the impedances in three major electric current paths of DSSCs, that is, the resistance of electrolytes and electric contacts, impedance across the electrolytes/dye‐coated TiO2 interface, and Nernstian diffusion within the electrolytes. The results were also consistent with the increased molar conductivity of nanocomposite‐gelled electrolytes. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 47–53, 2008
Highly stereo‐efficient synthesis and luminescence properties of novel trans‐regular vinylene– silylene–thiophene polymers
Authors: Majchrzak Mariusz , Ludwiczak Monika , Bayda Malgorzata , Marciniak Bronislaw , Marciniec Bogdan 
Published: November 2007

AbstractThe novel trans‐stereo‐regular silylene–thiophene derivatives (4, 5) with perfect consecutive silylene–arylene–silylene–vinylene linkage were synthesized via silylative coupling polycondensation of 2,5‐bis(vinyldimethylsilyl)thiophene (2) or 5,5′‐bis(vinyldimethylsilyl)‐2,2′‐bithiophene (3) catalyzed by ruthenium‐hydride complex [RuHCl(CO)(PCy3)2] (1). Their spectroscopic, absorption, and luminescence properties were characterized and compared with those of model compounds containing thiophene or bithiophene chromophores. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 127–137, 2008
Analysis of thermal degradation process of Nafion‐117 with age‐momentum correlation method
Authors: Shibahara Yuji , Akiyama Yoko , Izumi Yoshinobu , Nishijima Shigehiro , Honda Yoshihide , Kimura Norio , Tagawa Seiichi , Isoyama Goro 
Published: November 2007

AbstractThe thermal degradation of Nafion membrane was analyzed with age‐momentum correlation (AMOC) measurement and four‐electrode AC impedance measurement. In the heated sample, the decrease in proton conductivity was observed. The lifetimes and corresponding relative intensities showed fairly good agreement between heated and nonheated samples within the experimental error. In the analysis of the photopeak of annihilation γ‐ray, on the other hand, the difference between those two kinds of samples was observed, and this difference was found to be caused by the annihilation of free positrons with low energy electrons by using AMOC method. The decrease in proton conductivity was caused by the low energy electrons, namely sulfonic radicals. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 1–7, 2008
Structure and properties for transparent polypropylene containing sorbitol‐based clarifier
Authors: Tenma Miwa , Mieda Naoya , Takamatsu Shinya , Yamaguchi Masayuki 
Published: November 2007

AbstractRelation between structure and properties is studied for polypropylene (PP) containing 1,3:2,4‐di‐benzylidene sorbitol (DBS) that forms network structure composed of nanofibrils in a molten PP. It is found that the aggregation state of DBS, which can be controlled by the applied flow field and thermal history, affects the spherulite texture of PP and thus the transparency. When injection‐molded products, in which the nanofibrils of DBS orient to the flow direction, are reprocessed at 180 °C, that is, lower temperature than the melting point of DBS, the obtained material shows high level of transparency, although it has been believed that sorbitol‐derivatives have to be melted and dissolved into the molten PP at high temperature, for example, 240 °C. Further, it is found that the particle flow occurs in the blend at 180 °C. The applied shear force generates the particles, that is, flow units, by the fragmentation of the network structure. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 41–47, 2008
Sustaining hope when communicating with terminally ill patients and their families: a systematic review
Authors: Clayton Josephine M. , Hancock Karen , Parker Sharon , Butow Phyllis N. , Walder Sharon , Carrick Sue , Currow David , Ghersi Davina , Glare Paul , Hagerty Rebecca , Olver Ian N. , Tattersall Martin H. N. 
Published: November 2007

AbstractThe aim of this systematic review was to examine studies that have investigated sustaining hope during prognostic and end‐of‐life issues discussions with terminally ill patients and their families. A comprehensive search of databases (MEDLINE, EMBASE, CINAHL, PsychINFO, Cochrane Central Register of Controlled Trials) and handsearching, from 1985 to June 2006, identified 27 studies.This review suggests that the issues surrounding hope in this context are complex. Despite the lack of unanimity among researchers regarding the definition of hope, findings suggest that balancing hope with honesty is an important skill for health professionals (HPs). Many patients seem to be able to maintain a sense of hope despite acknowledging the terminal nature of their illness. Patients and caregivers mostly preferred honest and accurate information, provided with empathy and understanding. Many different sources of hope were identified in this context in broad aspects of life, not just the medical situation. HPs need to recognize this spectrum of hope and appreciate that patients may simultaneously hope for ‘cure’ while acknowledging the terminal nature of their illness. HPs may help patients to cope with their terminal prognosis by exploring and fostering realistic forms of hope that are meaningful for the particular patient and their family. Copyright © 2007 John Wiley & Sons, Ltd.
The development of multiple reaction monitoring assays for liver‐derived plasma proteins
Authors: McKay Matthew J. , Sherman Jamie , Laver Matthew T. , Baker Mark S. , Clarke Stephen J. , Molloy Mark P. 
Published: November 2007

AbstractThere is wide interpatient variability in toxicity to chemotherapeutic drugs and a lack of routine clinical tests for prospectively identifying patients at risk of developing toxicity from chemotherapy. An empirically driven MS strategy has been developed to monitor liver‐derived plasma proteins as potential biomarkers of early toxicity. Multiple reaction monitoring (MRM) has been used to assess 46 candidate peptides from 18 liver‐derived proteins. Following an iterative process of assay design, optimisation and assessment we selected 29 MRM assays (median CV 4.6%, range 1.2–11.6%) and monitored changes in levels of plasma proteins from a small number of colorectal cancer (CRC) patients undergoing chemotherapy. We demonstrated MRM assay robustness, and show that patients undergo minor elevation in plasma proteins when profiled on Day 3 of the chemotherapeutic regime. The MRM assays were in general agreement with 2‐D DIGE‐based quantitation from the same patient samples. The data supports the application of MRM‐based methods as facile, highly reproducible, medium‐throughput techniques that warrant expanded investigation for clinical utility in identifying patients at risk of developing chemotoxicity.
Salivary biomarkers associated with perceived satiety and body mass in humans
Authors: Harthoorn Lucien F. , Schipper Raymond G. , Loof Arnoud , Vereijken Pieter F. G. , Van Heerde Waander L. , Dransfield Eric 
Published: November 2007

AbstractRegulation of food intake and energy homeostasis is controlled by a delicate balancing of numerous central and peripheral factors, including circulating peptide hormones. This study investigated the proteome of saliva using SELDI‐TOF‐MS in relation to satiety and body mass index (BMI) in humans. Within a longitudinal test session, 18 subjects were exposed to a lunch‐induced hunger‐satiety shift, while every 15 min collecting their whole saliva and rating their hunger and satiety. Saliva was analysed by SELDI‐TOF‐MS using IMAC arrays with a chromatographic copper surface (IMAC‐Cu). From all subjects and time points measured, peptide and protein profiles showed 190 common peaks. Their interrelationships show that 37% of the variation was accounted for in one dimension. About 30 means had a strong association (0.70<|r|<0.95) with all subjective satiety ratings across time during the test session, and seven peaks were significantly correlated to BMI. Database MS searches indicated characterisation of some relevant metabolic peptide hormones. In conclusion, SELDI‐TOF‐MS on human saliva provides a valuable and noninvasive way of profiling that enables characterisation of novel and differently expressed peptides and proteins which can be used as biomarkers of satiety and overweight.