bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening H3 Histone Acetylation in a Wild Bird, the House Sparrow (Passer Domesticus)
Authors: Ray D , Sheldon E L , Zimmer C , Martin L B , Schrey A W 
Published: February 2024

SynopsisEpigenetic mechanisms are increasingly understood to have major impacts across ecology. However, one molecular epigenetic mechanism, DNA methylation, currently dominates the literature. A second mechanism, histone modification, is likely important to ecologically relevant phenotypes and thus warrants investigation, especially because molecular interplay between methylation and histone acetylation can strongly affect gene expression. There are a limited number of histone acetylation studies on non-model organisms, yet those that exist show that it can impact gene expression and phenotypic plasticity. Wild birds provide an excellent system to investigate histone acetylation, as free-living individuals must rapidly adjust to environmental change. Here, we screen histone acetylation in the house sparrow (Passer domesticus); we studied this species because DNA methylation was important in the spread of this bird globally. This species has one of the broadest geographic distributions in the world, and part of this success is related to the way that it uses methylation to regulate its gene expression. Here, we verify that a commercially available assay that was developed for mammals can be used in house sparrows. We detected high variance in histone acetylation among individuals in both liver and spleen tissue. Further, house sparrows with higher epigenetic potential in the Toll Like Receptor-4 (TLR-4) promoter (i.e., CpG content) had higher histone acetylation in liver. Also, there was a negative correlation between histone acetylation in spleen and TLR-4 expression. In addition to validating a method for measuring histone acetylation in wild songbirds, this study also shows that histone acetylation is related to epigenetic potential and gene expression, adding a new study option for ecological epigenetics.
“Just Theater!”—How Self-Legitimation Practices Can Backfire in International Organizations
Authors: Christian Ben 
Published: February 2024

AbstractWorking in international organizations (IOs) is not always a pleasure. Contradictory external demands make it difficult to do the right thing, constant failures in the field lead to frustration, and hierarchical structures require “emotional labor” on the part of IO staff. While we know that IO leadership therefore engages in self-legitimation practices to strengthen employee motivation and maintain organizational cohesion, we know little about the actual results of these activities. To address this gap, I focus on the perceptions of “ordinary” IO employees. Based on seventy-five in-depth interviews with IO staff in two different IOs, the United Nations (UN) Secretariat and the Food and Agriculture Organization (FAO), I develop a typology of five different staff responses to internal legitimation. I show that leadership’s legitimation efforts can not only result in (i) acceptance and adoption of the given narratives but also lead to (ii) criticism, (iii) toleration, (iv) cynicism, or even (v) exit of IO employees. Thus, ironically, the very leadership activities that are supposed to foster internal stability can also backfire and contribute to the destabilization of the IO. Building on these findings, I discuss two factors that influence IO employees’ perception of self-legitimation practices and are crucial for their success: the credibility of IO leaders and the right balance between glossing over and self-criticism in their legitimation narratives. I conclude by arguing that, contrary to common belief, legitimation and critique are not irreconcilable opposites. Rather, self-criticism can be an integral part of successful legitimation.
The Supply and Demand of Rebel Governance
Authors: Florea Adrian , Malejacq Romain 
Published: February 2024

Abstract
A recent wave of civil conflict scholarship examines rebel governance, the process through which insurgent groups organize local affairs in areas under their control. While current research predominantly focuses on the supply side of rebel governance, the attention given to the demand side has been relatively limited. In this study, we take stock of recent scholarship on the dynamic relationship between rebels and civilians to theoretically explore the supply side of rebel governance and develop a new demand-side typology that captures the key factors influencing civilian preferences regarding the nature of rebel rule. Specifically, we argue that demand for rebel governance is mainly shaped by the interaction between civilians’ perceptions of the state and civilian–rebel compatibility, which we define as civilians’ perceptions of the compatibility of the rebel group with their own values, ideology, identity, and preferred modes of socio-political organization. To illustrate our main theoretical points, we draw upon insights gleaned from multiple insurgencies. Our study significantly enhances our understanding of how rebel–civilian interactions mold the fabric of political order in civil war environments.
Inferior, Yet Transformative: the User Experience with Robotic Vacuum Cleaners
Authors: Hertzum Morten 
Published: February 2024

Abstract
Robotic vacuum cleaners are a prime example of home automation and a rich source of information about how people experience it. On the basis of a 3-week diary study, this article compares householders’ user experience (UX) of robotic vacuum cleaners with their UX of three types of manual vacuum cleaner. The main finding is that robotic vacuum cleaners are inferior in use, yet transform vacuuming. While their inferiority is about their pragmatic qualities, their transformative power relates to their autonomy, agency and hedonic qualities. This contradictory UX involves that robotic vacuum cleaners perform worse or not at all on seven out of nine surfaces and, at the same time, appear to escalate cleanliness standards. The transformation of household chores such as vacuuming is ongoing and calls for further research on engineering and merging the pragmatic and hedonic aspects of UX.
Functional properties of Ganoderma lucidum supplementation in canine nutrition
Authors: Kayser Emanuela , Castaneda Paola L , Soto-Diaz Katiria , Steelman Andrew J , Murphy Alayna , Spindola Maria , He Fei , de Godoy Maria R C 
Published: February 2024

Abstract
Ganoderma lucidum (GL) is a mushroom that has been widely used in Asia for its immunostimulatory and anti-inflammatory capacity, which has been hypothesized to be attributed mainly to the recognition of its cell-surface patterns by cells of the immune system present in the gastrointestinal tract, resulting in a cascade of modulatory events. However, the nutraceutical properties of GL have not been tested in dogs. Forty adult beagles were used in a completely randomized design. The objective of the present study was to evaluate the effects of dietary inclusion of GL on peripheral blood mononuclear cells (PBMC; T cells, B cells, monocytes, and natural killers), vaccine response, nutrient digestibility, fecal fermentative end-products, and skin and coat quality of adult dogs. Dogs were fed a commercial dry extruded complete and balanced diet plus GL top-dressed daily upon feeding time. Four experimental treatments were used: 0% GL supplementation (control), 5 mg/kg BW of GL, 10 mg/kg BW of GL, or 15 mg/kg BW of GL. Following a 7 d adaptation to the control diet, dogs were fed their respective treatment diets for 28 d. They were challenged with vaccination of a modified live virus Canine Distemper, Adenovirus Type 1 (Hepatitis), Adenovirus Type 2, Parainfluenza, and Parvovirus and killed Rabies Virus on day 7 with blood collections on days 0, 14, and 28. The inclusion of GL in all dosages was well-accepted by all dogs, with no detrimental effect on macronutrient apparent total tract digestibility. There was a trend that the percentage of major histocompatibility II (MHC-II) from B cells was greater in dogs fed 15 mg/kg of GL (41.91%) compared to the control group (34.63%). The phagocytosis response tended to have treatment-by-time interaction among treatments; dogs fed 15 mg/kg of GL tended to have greater phagocytosis activity on day 28 than dogs from the control group and dogs fed 5 mg/kg of GL. The vaccine-specific serum immunoglobulin G (IgG) concentrations were higher in the group supplemented with 15 mg/kg of GL compared to treatment control 7 d after the vaccination for rabies. These data suggest that the inclusion of GL had no detrimental effects on any analyzed PBMC. Due to changes in immune parameters among treatments, GL may also exert beneficial immunostimulatory effects in healthy adult dogs when provided at a daily dose of 15 mg/ kg BW.
Loss of maternal calcitriol reversibly alters early offspring growth and skeletal development in mice
Authors: Hartery Sarah A , Kirby Beth J , Walker Emma C , Kaufmann Martin , Jones Glenville , St-Arnaud René , Sims Natalie A , Kovacs Christopher S 
Published: February 2024

Abstract
Ablation of Cyp27b1 eliminates calcitriol but does not disturb fetal mineral homeostasis or skeletal development. However, independent of fetal genotypes, maternal loss of Cyp27b1 altered fetal mineral and hormonal levels compared to offspring of WT dams. We hypothesized that these maternal influences would alter postnatal skeletal development. Cyp27b1 null and WT females were mated to bear only Cyp27b1+/− offspring. Forty-eight hours after birth, pups were cross-fostered to dams of the same or opposite genotype that bore them. Maternal and offspring samples were collected on days 21 (weaning) and 42. Offspring measurements included minerals and hormones, BMC by DXA, ash weight and mineral content, gene expression, 3-point bending tests, and microCT. Maternal lactational behavior was evaluated. Milk was analyzed for nutritional content. At day 21, offspring fostered by nulls, independent of birth dam, had ~20% lower weight, BMC, ash weight, and ash calcium than pups fostered by WT dams. Adjustment for body weight accounted for the lower BMC but not the lower ash weight and ash calcium. Hormones and serum/urine minerals did not differ across offspring groups. Offspring fostered by nulls had shorter femurs and lower cortical thickness, mean polar moment of inertia, cortical area, trabecular bone volume, and trabecular number. Dam lactational behaviors and milk nutritional content did not differ between groups. At day 42, body weight, ash weight, lengths, BMC, and tibial bone strength were no longer different between pups fostered by null vs WT dams. In summary, pups fostered by Cyp27b1 nulls, regardless of birth dam, have proportionately smaller skeletons at 21 d, impaired microstructure, but normal mineral homeostasis. The skeletal effects are largely recovered by day 42 (3 wk after weaning). In conclusion, maternal loss of calcitriol impairs early postnatal cortical bone growth and trabecular bone mass, but affected offspring catch up after weaning.
Imagining sustainable worlds: the potential of mythical stories in environmental education
Authors: Ikonen Essi , Keränen-Pantsu Raili , Welz Claudia 
Published: February 2024

Abstract
Pedagogically speaking, how can we best transform a student’s understanding of the environment? To move students to action, and to inspire sustainable lifestyles, environmental educators would do well to consider personal pedagogical approaches, as opposed to merely present scientific facts about climate change and species extinction. In this article, we present the power of myth as a compelling option. We expand on prevailing pedagogies of myth, such as Matthew Farrelly’s approach, and argue that mythical stories taken from Nordic folk traditions, the Hebrew Bible, the New Testament, and the Quran can enhance environmental education by introducing multiple alternative ways of relating to nature, facilitating mutual teacher–student reflections, and by building more responsive relationships with fauna and flora. Building upon Paul Ricoeur’s hermeneutical theory, we contend that the process of imagining sustainable worlds is facilitated by engagement with mythical stories and that the counter-productive imposition of world-views on students is minimized through critical discussions of relevant learning materials.
Emergent plant presence and richness alter competitive interactions between two floating plants
Authors: Liu Jun-Nan , Wu Fang-Ru , Roiloa Sergio R , Xue Wei , Lei Ning-Fei , Yu Fei-Hai 
Published: February 2024

Abstract
Interactions between two plant species can be influenced by the presence of other plant species and such an effect may change as the diversity of the other species increases. To test these hypotheses, we first constructed aquatic communities consisting of 1, 2 and 4 emergent plant species and then grew ramets of Lemna minor only, ramets of Spirodela polyrhiza only or ramets of both L. minor and S. polyrhiza within these aquatic communities. We also included controls with ramets of L. minor, S. polyrhiza or both but without any emergent plants. Biomass and number of ramets of L. minor and S. polyrhiza were significantly smaller with than without the emergent plants, but they did not differ among the three richness levels. The presence of S. polyrhiza did not significantly affect the growth of L. minor, and such an effect was not dependent on the richness of the emergent plant species. Without the emergent plant species, the presence of L. minor markedly reduced biomass (−92%) and number of ramets (−88%) of S. polyrhiza. However, such a competitive effect of L. minor on S. polyrhiza became much weaker in the presence of one emergent plant species (−46% biomass and −39% number of ramets) and completely disappeared in the presence of two or four emergent plant species. Therefore, both the presence and richness of emergent plant species can alter competitive interactions between the two duckweed species. These findings highlight the importance of species diversity in regulating plant–plant interactions.
Albumin–bilirubin score is a useful predictor of worsening liver reserve after stereotactic body radiation therapy in elderly Japanese patients with hepatocellular carcinoma
Authors: Yoshino Yuki , Suzuki Gen , Shiomi Hiroya , Kimoto Takuya , Seri Sho , Yamazaki Hideya , Yamada Kei 
Published: February 2024

Abstract
The prognosis of patients with hepatocellular carcinoma (HCC) is closely related to their liver reserves. The Child-Pugh (CP) score has traditionally been used to evaluate this reserve, with CP Grade B (CP score ≥ 7) associated with a higher risk of radiation-induced liver disease after stereotactic body radiation therapy (SBRT). However, the CP score has limitations, as it does not accurately assess liver reserve capacity. The albumin–bilirubin (ALBI) score has been introduced as a meticulous indicator of liver reserve for the treatment of HCC. We retrospectively evaluated the role of the ALBI score in estimating the worsening liver reserve in 42 patients with HCC treated with SBRT using CyberKnife between 2015 and 2023. The median biologically effective dose (α/β = 10 Gy) was 100 Gy. For a median follow-up duration of 17.4 months, the 1-year overall survival (OS), local control (LC) and progression-free survival (PFS) rates were 100, 98 and 62%, respectively. Worsening liver reserve was defined as an increase in the modified ALBI grade or CP score within 1 year after SBRT. Univariate and multivariate analyses showed that the baseline ALBI score (≥−2.7 vs &lt;−2.7) was the only significantly different predictor of worsening liver reserve. The OS and LC rates after SBRT for HCC were satisfactory. However, the PFS was poor, and recurrent HCC will require additional treatment. It is clinically important to predict the liver reserve capacity after SBRT, and the baseline ALBI score is a useful predictor.
Waking up Sleeping Beauty: DNA damage activates dormant stem cell division by enhancing brassinosteroid signaling
Authors: Heyman Jefri , De Veylder Lieven 
Published: February 2024

This article comments on:
Takahashi N, Suita K, Koike T, Ogita N, Zhang Y, Umeda M. 2024. DNA double-strand breaks enhance brassinosteroid signaling to activate quiescent center cell division in Arabidopsis. Journal of Experimental Botany 75, 1364–1375.
Functional and Evolutionary Integration of a Fungal Gene With a Bacterial Operon
Authors: Sun Liang , David Kyle T , Wolters John F , Karlen Steven D , Gonçalves Carla , Opulente Dana A , LaBella Abigail Leavitt , Groenewald Marizeth , Zhou Xiaofan , Shen Xing-Xing , Rokas Antonis , Hittinger Chris Todd 
Published: February 2024

Abstract
Siderophores are crucial for iron-scavenging in microorganisms. While many yeasts can uptake siderophores produced by other organisms, they are typically unable to synthesize siderophores themselves. In contrast, Wickerhamiella/Starmerella (W/S) clade yeasts gained the capacity to make the siderophore enterobactin following the remarkable horizontal acquisition of a bacterial operon enabling enterobactin synthesis. Yet, how these yeasts absorb the iron bound by enterobactin remains unresolved. Here, we demonstrate that Enb1 is the key enterobactin importer in the W/S-clade species Starmerella bombicola. Through phylogenomic analyses, we show that ENB1 is present in all W/S clade yeast species that retained the enterobactin biosynthetic genes. Conversely, it is absent in species that lost the ent genes, except for Starmerella stellata, making this species the only cheater in the W/S clade that can utilize enterobactin without producing it. Through phylogenetic analyses, we infer that ENB1 is a fungal gene that likely existed in the W/S clade prior to the acquisition of the ent genes and subsequently experienced multiple gene losses and duplications. Through phylogenetic topology tests, we show that ENB1 likely underwent horizontal gene transfer from an ancient W/S clade yeast to the order Saccharomycetales, which includes the model yeast Saccharomyces cerevisiae, followed by extensive secondary losses. Taken together, these results suggest that the fungal ENB1 and bacterial ent genes were cooperatively integrated into a functional unit within the W/S clade that enabled adaptation to iron-limited environments. This integrated fungal-bacterial circuit and its dynamic evolution determine the extant distribution of yeast enterobactin producers and cheaters.
From “Additive-Free” to “Tobacco & Water”: Assessing the Impact of Marketing Claim Changes on Natural American Spirit and L&M Cigarettes
Authors: Weiger Caitlin , Gratale Stefanie K , Ganz Ollie , Wackowski Olivia A 
Published: February 2024

Abstract

Introduction
The 2017 agreement between the U.S. Food and Drug Administration and the makers of Natural America Spirit (NAS) cigarettes restricted the use of “additive-free” (AF) in brand marketing, which the company replaced with “tobacco ingredients: tobacco & water” (TW), a phrase subsequently adopted by L&M. We tested how participants’ perceptions differed when exposed to AF versus TW claims on NAS and L&M packs.

Aims and Methods
A between-subjects experiment was embedded within an online survey in August 2022 (n = 2526). Participants were randomized to view one of three packs (NAS AF pack, NAS TW pack, L&M TW pack). Logistic regressions assessed differences (by pack exposure) in perceived pack attractiveness and relative chemical and harm perceptions compared to other cigarettes. Interactions by smoking status were explored.

Results
No significant differences were observed between the NAS AF and NAS TW packs on perceived attractiveness or relative harm, although never smokers had higher odds of having lower relative chemicals perceptions in response to the NAS AF versus NAS TW pack (OR = 1.81). Compared to participants who saw the NAS TW pack, those who saw the L&M TW pack tended to have lower odds of perceiving the pack as attractive, believing that the product had fewer chemicals than other products, or believing the product was less harmful than other products compared to the NAS TW pack.

Conclusion
Results suggest the more implicit “tobacco ingredients: tobacco & water” claim functions similarly to “additive-free” and as an unauthorized modified risk claim. NAS brand image also appears to contribute to higher perceived pack attractiveness and lower perceptions of chemicals and harm. Additional marketing regulations and corrective campaigns may be needed to reduce risk-related misperceptions about NAS cigarettes.

Implications
Our findings show that replacing “additive-free” claims with “tobacco ingredients: tobacco & water” did not sufficiently address the illegal use of unauthorized modified risk claims by the makers of NAS cigarettes. Further regulatory action restricting the TW claim could potentially reduce inaccurate lower harm perceptions about NAS cigarettes. Educational campaigns may also be needed to mitigate NAS’s history of marketing that implies reduced harm, given that the effects of implicit reduced risk claims like TW have a greater effect on pack perceptions for NAS than L&M.
Effects of physical forcing on short-term plankton dynamics in a narrow coral reef lagoon (Ouano, New Caledonia): a two-week high-frequency study
Authors: Rodier Martine , Pagano Marc , Lhomond Lucas , Péricaud Jean-Baptiste , Guilloux Loïc , Devenon Jean-Luc , Chevalier Cristèle 
Published: February 2024

Abstract
The response of plankton communities to short-term climatic events was studied in a narrow coral reef lagoon representative of the channel-type lagoons of the high islands of the tropical Pacific. Phyto- and zooplankton data (abundance and taxonomic composition) were sampled at high frequencies for 2 weeks in April–May 2017 under various tidal conditions and combined with environmental and physical measurements and modeling hydrodynamic products. The short-term external events (wind, waves and rain) that occurred caused changes in the circulation patterns and increased nutrient concentrations in the lagoon. The plankton reacted quickly to this enrichment, with an increase in chlorophyll a and the rapid development of a microphytoplankton community dominated by diatoms, and of opportunistic herbivorous zooplankton (tintinnids, appendicularians). Zooplankton composition and distribution were strongly modulated by the circulation patterns through advection and mixing. Our results show that the tidal and diel components of the temporal variability of planktonic groups were blurred by sporadic event components (i.e. wind, wave and rainfall events). Environmental and biological responses to these external physical forcings occurred at lagoon scale in this channel-like lagoon, unlike what is observed in larger lagoons (such as the southern lagoons of New Caledonia) where spatial variability is much higher.