bluuebunny/crossref_metadata_2025_split huggingface dataset:
IGF-1-mediated FOXC1 overexpression induces stem-like properties through upregulating CBX7 and IGF-1R in esophageal squamous cell carcinoma
Authors: Wu Hao , Li Zhao-Xing , Fang Kang , Zhao Zi-Ying , Sun Ming-Chuang , Feng An-Qi , Leng Zhu-Yun , Zhang Ze-Hua , Chu Yuan , Zhang Li , Chen Tao , Xu Mei-Dong 
Published: February 2024

AbstractSubstantial evidence attests to the pivotal role of cancer stem cells (CSC) in both tumorigenesis and drug resistance. A member of the forkhead box (FOX) family, FOXC1, assumes significance in embryonic development and organogenesis. Furthermore, FOXC1 functions as an overexpressed transcription factor in various tumors, fostering proliferation, enhancing migratory capabilities, and promoting drug resistance, while maintaining stem-cell-like properties. Despite these implications, scant attention has been devoted to its role in esophageal squamous cell carcinoma. Our investigation revealed a pronounced upregulation of FOXC1 expression in ESCC, correlating with a poor prognosis. The downregulation of FOXC1 demonstrated inhibitory effects on ESCC tumorigenesis, proliferation, and tolerance to chemotherapeutic agents, concurrently reducing the levels of stemness-related markers CD133 and CD44. Further studies validated that FOXC1 induces ESCC stemness by transactivating CBX7 and IGF-1R. Additionally, IGF-1 activated the PI3K/AKT/NF-κB and MEK/ERK/NF-κB pathways through its binding to IGF-1R, thereby augmenting FOXC1 expression. Conversely, suppressing FOXC1 impeded ESCC stemness induced by IGF-1. The presence of a positive feedback loop, denoted by IGF-1-FOXC1-IGF-1R, suggests the potential of FOXC1 as a prognostic biomarker for ESCC. Taken together, targeting the IGF-1-FOXC1-IGF-1R axis emerges as a promising approach for anti-CSC therapy in ESCC.
Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury
Authors: Jo-Watanabe Airi , Inaba Toshiki , Osada Takahiro , Hashimoto Ryota , Nishizawa Tomohiro , Okuno Toshiaki , Ihara Sayoko , Touhara Kazushige , Hattori Nobutaka , Oh-Hora Masatsugu , Nureki Osamu , Yokomizo Takehiko 
Published: February 2024

AbstractHomoeostatic regulation of the acid–base balance is essential for cellular functional integrity. However, little is known about the molecular mechanism through which the acid–base balance regulates cellular responses. Here, we report that bicarbonate ions activate a G protein-coupled receptor (GPCR), i.e., GPR30, which leads to Gq-coupled calcium responses. Gpr30-Venus knock-in mice reveal predominant expression of GPR30 in brain mural cells. Primary culture and fresh isolation of brain mural cells demonstrate bicarbonate-induced, GPR30-dependent calcium responses. GPR30-deficient male mice are protected against ischemia-reperfusion injury by a rapid blood flow recovery. Collectively, we identify a bicarbonate-sensing GPCR in brain mural cells that regulates blood flow and ischemia–reperfusion injury. Our results provide a perspective on the modulation of GPR30 signalling in the development of innovative therapies for ischaemic stroke. Moreover, our findings provide perspectives on acid/base sensing GPCRs, concomitantly modulating cellular responses depending on fluctuating ion concentrations under the acid–base homoeostasis.
Latent ion tracks were finally observed in diamond
Authors: Amekura H. , Chettah A. , Narumi K. , Chiba A. , Hirano Y. , Yamada K. , Yamamoto S. , Leino A. A. , Djurabekova F. , Nordlund K. , Ishikawa N. , Okubo N. , Saitoh Y. 
Published: February 2024

AbstractInjecting high-energy heavy ions in the electronic stopping regime into solids can create cylindrical damage zones called latent ion tracks. Although these tracks form in many materials, none have ever been observed in diamond, even when irradiated with high-energy GeV uranium ions. Here we report the first observation of ion track formation in diamond irradiated with 2–9 MeV C60 fullerene ions. Depending on the ion energy, the mean track length (diameter) changed from 17 (3.2) nm to 52 (7.1) nm. High resolution scanning transmission electron microscopy (HR-STEM) indicated the amorphization in the tracks, in which π-bonding signal from graphite was detected by the electron energy loss spectroscopy (EELS). Since the melting transition is not induced in diamond at atmospheric pressure, conventional inelastic thermal spike calculations cannot be applied. Two-temperature molecular dynamics simulations succeeded in the reproduction of both the track formation under MeV C60 irradiations and the no-track formation under GeV monoatomic ion irradiations.
Chorus wave power at the strong diffusion limit overcomes electron losses due to strong diffusion
Authors: Daggitt T. A. , Horne R. B. , Glauert S. A. , Del Zanna G. , Albert J. M. 
Published: February 2024

AbstractEarth’s radiation belts consist of high-energy charged particles trapped by Earth’s magnetic field. Strong pitch angle diffusion of electrons caused by wave-particle interaction in Earth’s radiation belts has primarily been considered as a loss process, as trapped electrons are rapidly diffused into the loss cone and lost to the atmosphere. However, the wave power necessary to produce strong diffusion should also produce rapid energy diffusion, and has not been considered in this context. Here we provide evidence of strong diffusion using satellite data. We use two-dimensional Fokker-Planck simulations of electron diffusion in pitch angle and energy to show that scaling up chorus wave power to the strong diffusion limit produces rapid acceleration of electrons, sufficient to outweigh the losses due to strong diffusion. The rate of losses saturates at the strong diffusion limit, whilst the rate of acceleration does not. This leads to the surprising result of an increase, not a decrease in the trapped electron population during strong diffusion due to chorus waves as expected when treating strong diffusion as a loss process. Our results suggest there is a tipping point in chorus wave power between net loss and net acceleration that global radiation belt models need to capture to better forecast hazardous radiation levels that damage satellites.
Single-pixel p-graded-n junction spectrometers
Authors: Wang Jingyi , Pan Beibei , Wang Zi , Zhang Jiakai , Zhou Zhiqi , Yao Lu , Wu Yanan , Ren Wuwei , Wang Jianyu , Ji Haiming , Yu Jingyi , Chen Baile 
Published: February 2024

AbstractUltra-compact spectrometers are becoming increasingly popular for their promising applications in biomedical analysis, environmental monitoring, and food safety. In this work, we report a single-pixel-photodetector spectrometer with a spectral range from 480 nm to 820 nm, based on the AlGaAs/GaAs p-graded-n junction with a voltage-tunable optical response. To reconstruct the optical spectrum, we propose a tailored method called Neural Spectral Fields (NSF) that leverages the unique wavelength and bias-dependent responsivity matrix. Our spectrometer achieves a high spectral wavelength accuracy of up to 0.30 nm and a spectral resolution of up to 10 nm. Additionally, we demonstrate the high spectral imaging performance of the device. The compatibility of our demonstration with the standard III-V process greatly accelerates the commercialization of miniaturized spectrometers.
Observation of continuum Landau modes in non-Hermitian electric circuits
Authors: Zhang Xuewei , Wu Chaohua , Yan Mou , Liu Ni , Wang Ziyu , Chen Gang 
Published: February 2024

AbstractContinuum Landau modes — predicted recently in a non-Hermitian Dirac Hamiltonian under a uniform magnetic field — are continuous bound states with no counterparts in Hermitian systems. However, they have still not been confirmed in experiments. Here, we report an experimental observation of continuum Landau modes in non-Hermitian electric circuits, in which the non-Hermitian Dirac Hamiltonian is simulated by non-reciprocal hoppings and the pseudomagnetic field is introduced by inhomogeneous complex on-site potentials. Through measuring the admittance spectrum and the eigenstates, we successfully verify key features of continuum Landau modes. Particularly, we observe the exotic voltage response acting as a rainbow trap or wave funnel through full-field excitation. This response originates from the linear relationship between the modes’ center position and complex eigenvalues. Our work builds a bridge between non-Hermiticity and magnetic fields, and thus opens an avenue to explore exotic non-Hermitian physics.
Reconfigurable perovskite X-ray detector for intelligent imaging
Authors: Pang Jincong , Wu Haodi , Li Hao , Jin Tong , Tang Jiang , Niu Guangda 
Published: February 2024

AbstractX-ray detection is widely used in various applications. However, to meet the demand for high image quality and high accuracy diagnosis, the raw data increases and imposes challenges for conventional X-ray detection hardware regarding data transmission and power consumption. To tackle these issues, we present a scheme of in-X-ray-detector computing based on CsPbBr3 single-crystal detector with convenient polarity reconfigurability, good linear dynamic range, and robust stability. The detector features a stable trap-free device structure and achieves a high linear dynamic range of 106 dB. As a result, the detector could achieve edge extraction imaging with a data compression ratio of ~50%, and could also be programmed and trained to perform pattern recognition tasks with a high accuracy of 100%. Our research shows that in-X-ray-detector computing can be used in flexible and complex scenarios, making it a promising platform for intelligent X-ray imaging.
Immunisation efficacy of a stabilised SARS-CoV-2 spike glycoprotein in two geriatric animal models
Authors: Usai Carla , Ainsua-Enrich Erola , Gales Victor Urrea , Pradenas Edwards , Lorca-Oró Cristina , Tarrés-Freixas Ferran , Roca Núria , Pérez Mónica , Ávila-Nieto Carlos , Rodríguez de la Concepción María Luisa , Pedreño-Lopez Núria , Carabelli Julieta , Trinité Benjamin , Ballana Ester , Riveira-Muñoz Eva , Izquierdo-Useros Nuria , Clotet Bonaventura , Blanco Julià , Guallar Victor , Cantero Guillermo , Vergara-Alert Júlia , Carrillo Jorge , Segalés Joaquim 
Published: February 2024

AbstractAge is associated with reduced efficacy of vaccines and linked to higher risk of severe COVID-19. Here we determined the impact of ageing on the efficacy of a SARS-CoV-2 vaccine based on a stabilised Spike glycoprotein (S-29) that had previously shown high efficacy in young animals. Thirteen to 18-month-old golden Syrian hamsters (GSH) and 22–23-month-old K18-hCAE2 mice were immunised twice with S-29 protein in AddaVaxTM adjuvant. GSH were intranasally inoculated with SARS-CoV-2 either two weeks or four months after the booster dose, while all K18-hACE2 mice were intranasally inoculated two weeks after the second immunisation. Body weight and clinical signs were recorded daily post-inoculation. Lesions and viral load were investigated in different target tissues. Immunisation induced seroconversion and production of neutralising antibodies; however, animals were only partially protected from weight loss. We observed a significant reduction in the amount of viral RNA and a faster viral protein clearance in the tissues of immunized animals. Infectious particles showed a faster decay in vaccinated animals while tissue lesion development was not altered. In GSH, the shortest interval between immunisation and inoculation reduced RNA levels in the lungs, while the longest interval was equally effective in reducing RNA in nasal turbinates; viral nucleoprotein amount decreased in both tissues. In mice, immunisation was able to improve the survival of infected animals. Despite the high protection shown in young animals, S-29 efficacy was reduced in the geriatric population. Our research highlights the importance of testing vaccine efficacy in older animals as part of preclinical vaccine evaluation.
MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques
Authors: Beddingfield Brandon J. , Plante Kenneth S. , Plante Jessica A. , Weaver Scott C. , Bose Sarah , Krzykwa Clara , Chirichella Nicole , Redmann Rachel K. , Seiler Stephanie Z. , Dufour Jason , Blair Robert V. , Endt Kathrin , Volkmann Ariane , Maness Nicholas J. , Roy Chad J. 
Published: February 2024

AbstractMVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus (MVA-BN-WEV) vaccines were evaluated in the cynomolgus macaque aerosol model of EEEV infection. Macaques vaccinated with two doses of 5 × 108 infectious units of the MVA-BN-EEEV or MVA-BN-WEV vaccine by the intramuscular route rapidly developed robust levels of neutralizing antibodies to EEEV that persisted at high levels until challenge at day 84 via small particle aerosol delivery with a target inhaled dose of 107 PFU of EEEV FL93-939. Robust protection was observed, with 7/8 animals receiving MVA-BN-EEEV and 100% (8/8) animals receiving MVA-BN-WEV surviving while only 2/8 mock vaccinated controls survived lethal challenge. Complete protection from viremia was afforded by both vaccines, with near complete protection from vRNA loads in tissues and any pathologic evidence of central nervous system damage. Overall, the results indicate both vaccines are effective in eliciting an immune response that is consistent with protection from aerosolized EEEV-induced disease.
BANKSY unifies cell typing and tissue domain segmentation for scalable spatial omics data analysis
Authors: Singhal Vipul , Chou Nigel , Lee Joseph , Yue Yifei , Liu Jinyue , Chock Wan Kee , Lin Li , Chang Yun-Ching , Teo Erica Mei Ling , Aow Jonathan , Lee Hwee Kuan , Chen Kok Hao , Prabhakar Shyam 
Published: February 2024

AbstractSpatial omics data are clustered to define both cell types and tissue domains. We present Building Aggregates with a Neighborhood Kernel and Spatial Yardstick (BANKSY), an algorithm that unifies these two spatial clustering problems by embedding cells in a product space of their own and the local neighborhood transcriptome, representing cell state and microenvironment, respectively. BANKSY’s spatial feature augmentation strategy improved performance on both tasks when tested on diverse RNA (imaging, sequencing) and protein (imaging) datasets. BANKSY revealed unexpected niche-dependent cell states in the mouse brain and outperformed competing methods on domain segmentation and cell typing benchmarks. BANKSY can also be used for quality control of spatial transcriptomics data and for spatially aware batch effect correction. Importantly, it is substantially faster and more scalable than existing methods, enabling the processing of millions of cell datasets. In summary, BANKSY provides an accurate, biologically motivated, scalable and versatile framework for analyzing spatially resolved omics data.
Harmonized diffusion MRI data and white matter measures from the Adolescent Brain Cognitive Development Study
Authors: Cetin-Karayumak Suheyla , Zhang Fan , Zurrin Ryan , Billah Tashrif , Zekelman Leo , Makris Nikos , Pieper Steve , O’Donnell Lauren J. , Rathi Yogesh 
Published: February 2024

AbstractThe Adolescent Brain Cognitive Development (ABCD) Study® has collected data from over 10,000 children across 21 sites, providing insights into adolescent brain development. However, site-specific scanner variability has made it challenging to use diffusion MRI (dMRI) data from this study. To address this, a dataset of harmonized and processed ABCD dMRI data (from release 3) has been created, comprising quality-controlled imaging data from 9,345 subjects, focusing exclusively on the baseline session, i.e., the first time point of the study. This resource required substantial computational time (approx. 50,000 CPU hours) for harmonization, whole-brain tractography, and white matter parcellation. The dataset includes harmonized dMRI data, 800 white matter clusters, 73 anatomically labeled white matter tracts in full and low resolution, and 804 different dMRI-derived measures per subject (72.3 TB total size). Accessible via the NIMH Data Archive, it offers a large-scale dMRI dataset for studying structural connectivity in child and adolescent neurodevelopment. Additionally, several post-harmonization experiments were conducted to demonstrate the success of the harmonization process on the ABCD dataset.
A dataset for predicting cloud cover over Europe
Authors: Svennevik Hanna , Hicks Steven A. , Riegler Michael A. , Storelvmo Trude , Hammer Hugo L. 
Published: February 2024

AbstractClouds are important factors when projecting future climate. Unfortunately, future cloud fractional cover (the portion of the sky covered by clouds) is associated with significant uncertainty, making climate projections difficult. In this paper, we present the European Cloud Cover dataset, which can be used to learn statistical relations between cloud cover and other environmental variables, to potentially improve future climate projections. The dataset was created using a novel technique called Area Weighting Regridding Scheme to map satellite observations to cloud fractional cover on the same grid as the other variables in the dataset. Baseline experiments using autoregressive models document that it is possible to use the dataset to predict cloud fractional cover.
A high altitude respiration and SpO2 dataset for assessing the human response to hypoxia
Authors: Zhang Xi , Zhang Yu , Si Yingjun , Gao Nan , Zhang Honghao , Yang Hui 
Published: February 2024

AbstractThis report presents the Harespod dataset, an open dataset for high altitude hypoxia research, which includes respiration and SpO2 data. The dataset was collected from 15 college students aged 23–31 in a hypobaric oxygen chamber, during simulated altitude changes and induced hypoxia. Real-time physiological data, such as oxygen saturation waveforms, oxygen saturation, respiratory waveforms, heart rate, and pulse rate, were obtained at 100 Hz. Approximately 12 hours of valid data were collected from all participants. Researchers can easily identify the altitude corresponding to physiological signals based on their inherent patterns. Time markers were also recorded during altitude changes to facilitate realistic annotation of physiological signals and analysis of time-difference-of-arrival between various physiological signals for the same altitude change event. In high altitude scenarios, this dataset can be used to enhance the detection of human hypoxia states, predict respiratory waveforms, and develop related hardware devices. It will serve as a valuable and standardized resource for researchers in the field of high altitude hypoxia research, enabling comprehensive analysis and comparison.
A database on the abundance of environmental antibiotic resistance genes
Authors: Xu Wenjuan , Pan Zhizhen , Wu Yangyu , An Xin-Li , Wang Weiyi , Adamovich Boris , Zhu Yong-Guan , Su Jian-Qiang , Huang Qiansheng 
Published: February 2024

AbstractAntimicrobial resistance (AMR) poses a severe threat to global health. The wide distribution of environmental antibiotic resistance genes (ARGs), which can be transferred between microbiota, especially clinical pathogens and human commensals, contributed significantly to AMR. However, few databases on the spatiotemporal distribution, abundance, and health risk of ARGs from multiple environments have been developed, especially on the absolute level. In this study, we compiled the ARG occurrence data generated by a high-throughput quantitative PCR platform from 1,403 samples in 653 sampling sites across 18 provinces in China. The database possessed 291,870 records from five types of habitats on the abundance of 290 ARGs, as well as 8,057 records on the abundance of 30 mobile genetic elements (MGEs) from 2013 to 2020. These ARGs conferred resistance to major common types of antibiotics (a total of 15 types) and represented five major resistance mechanisms, as well as four risk ranks. The database can provide information for studies on the dynamics of ARGs and is useful for the health risk assessment of AMR.
MicroRNA-203a inhibits breast cancer progression through the PI3K/Akt and Wnt pathways
Authors: Entezari Maryam , Soltani Bahram M. , Sadeghizadeh Majid 
Published: February 2024

AbstractMicroRNA expression in breast cancer (BC) is explored both as a potential biomarker and for therapeutic purposes. Recent studies have revealed that miR-203a-3p is involved in BC, and importantly contributes to BC chemotherapy responses; however, the regulatory pathways of miR-203a in BC remain elusive. Hence, we aimed to investigate the miR-203a regulatory mechanisms and their potential functions in the progress of BC. To this end, the miR-203a potential involving pathways was predicted by databases analyzing its target genes. The relations between miR-203a, the phosphatidylinositol 3′-kinase (PI3K)–Akt, and Wnt signaling pathways were mechanistically investigated. Our results revealed that miR-203a inhibited the activation of the PI3K/Akt and Wnt pathways and reduced its downstream cell cycle signals, including Cyclin D1 and c-Myc. Moreover, the overexpression of miR-203a drastically arrested the cell cycle at subG1 and G1 phases, decreased the viability, proliferation, and migration, and increased apoptosis of BC cells. Therefore, miR-203a-3p may be considered a tumor suppressor factor and a potential biomarker or therapeutic target for BC.
Silicon attenuates aluminum toxicity in sugarcane plants by modifying growth, roots morphoanatomy, photosynthetic pigments, and gas exchange parameters
Authors: da Silveira Sousa Junior Gilmar , Hurtado Alexander Calero , de Cassia Alves Rita , Gasparino Eduardo Custodio , dos Santos Durvalina Maria Mathias 
Published: February 2024

AbstractAluminum (Al) inhibits growth and limits plant productivity in acidic soils. An important strategy to increase Al tolerance is the use of silicon (Si) nutrition. Thus, the aim of this study was to evaluate the interactive role of Si in increasing the growth, physiological and morphoanatomy responses of sugarcane plants under Al toxicity. A 4 × 2 factorial scheme in a completely randomized design was used to study the impact of Si (2 mM) on attenuating Al toxicity (0, 10, 15 and 20 mg L−1, as Al2(SO4)3·18H2O) in sugarcane seedlings. After 45 days, Al toxicity affected sugarcane growth by increasing Al uptake and accumulation, modifying root growth, thickness, and morphoanatomy, and decreasing pigment content, gas exchange parameters, and the number of adaxial and abaxial stomata. However, Si attenuated Al toxicity in the sugarcane seedlings by limiting Al uptake and transport to the shoots, causing positive changes in root morphoanatomy, higher pigment content, improving gas exchange parameters, thereby increased growth. Furthermore, cultivar ‘CTC9003’ showed beneficial impacts from Si supplementation than ‘CTC9002’, especially under Al toxicity. The findings of this study suggest that Si plays a notable role in improving anatomical and physiological aspects, particularly the growth of sugarcane seedlings under Al toxicity.