bluuebunny/crossref_metadata_2025_split huggingface dataset:
The evolution of Envelope function during coinfection with phylogenetically distinct Human Immunodeficiency Virus
Authors: Omar Shatha , Woodman Zenda L. 
Published: March 2024

Abstract

Background Coinfection with two phylogenetically distinct Human Immunodeficiency Virus-1 (HIV-1) variants might provide an opportunity for rapid viral expansion and the emergence of fit variants that drive disease progression. However, autologous neutralising immune responses are known to drive Envelope (Env) diversity which can either enhance replicative capacity, have no effect, or reduce viral fitness. This study investigated whether in vivo outgrowth of coinfecting variants was linked to pseudovirus and infectious molecular clones’ infectivity to determine whether diversification resulted in more fit virus with the potential to increase disease progression.Results For most participants, emergent recombinants displaced the co-transmitted variants and comprised the major population at 52 weeks post-infection with significantly higher entry efficiency than other co-circulating viruses. Our findings suggest that recombination within gp41 might have enhanced Env fusogenicity which contributed to the increase in pseudovirus entry efficiency. Finally, there was a significant correlation between pseudovirus entry efficiency and CD4 + T cell count, suggesting that the enhanced replicative capacity of recombinant variants could result in more virulent viruses.Conclusion Co-infection provides variants with the opportunity to undergo rapid recombination that results in more infectious virus. This highlights the importance of monitoring the replicative fitness of emergent viruses.
RETRACTED: MiR-145 modulation of TGF-β pathway attenuates EMT inhibition of Wilms’Tumor cell proliferation, invasion and migration
Authors: Zhou Jun , Wu Zhiwei , Zhu Jian , Song Jialun , Wang Peng , Zhao Xinxin , Liu Ziping , Lu Songxian , Shi Hongguang , Feng Xiaojuan , Zheng Mingjun , Du Junpeng 
Published: March 2024

Abstract

The authors have requested that this preprint be removed from Research Square.
The Ties That Bind: Exploring Place Identity and Attachment Among Digital Nomads
Authors: Lagidze Nato , Pirtskhalava Ekaterine 
Published: March 2024

Abstract

Place identity and place attachment are central concepts in environmental psychology that are especially relevant in the current era of enhanced international mobility. Recently, Georgia has emerged as a hotspot for ‘digital nomads.’ This mixed-methods research undertakes a comparative analysis of place identity and place attachment among Georgian residents and ‘digital nomads’ connected to Georgia. Study 1 focused on qualitative analysis through 16 in-depth interviews, comprising both Georgian participants and international digital nomads. This phase aimed to understand personal experiences, adaptation strategies, and the impact of mobility on place identity and attachment. Themes from these in-depth interviews were categorized into past, present, and future perspectives. The qualitative insights revealed significant factors that shape place identity among people on the move, including cultural, social, and environmental elements, as well as participants' past experiences and future aspirations. In Study 2, quantitative analysis was conducted with a sample of 176 individuals, using an online self-report questionnaire. The quantitative results highlighted the relationships between place identity, place attachment, and psychological well-being and life satisfaction.
Insights on Lipid Biodegradation in Domestic Biodegradable Waste at a Full-scale Black Soldier Fly Larvae (Hermetia illucens L.) Bioconversion
Authors: Fan Shilin , Ma Jingjin , Jiang Shuoyun , Khan Faw , Xiang FA , Zhijian zhang 
Published: March 2024

Abstract
The lipids in the domestic biodegradable waste (DBW) pose a challenge to resource regeneration, and few studies have examined the evolution of lipid profiles during the process of black soldier fly larvae (Hermetia illucens L., BSFL) bioconversion. This study aimed to explore the dynamic features of lipid fraction and their associated responses of microbial community succession in residue during a full-scale BSFL bioconversion. Data showed that the lipid content decreased by95%, while the seed germination index increased by 20% through the synergistic effects of BSFL and microbiota. The results of spectral and Gas chromatography-mass spectrometry showed that free fatty acids and medium-chain fatty acids were given first priority in degrading in larval and microbial coexistence systems, resulting in the relative accumulation of sterols. The lipid content (71.1%, P = 0.002) was the prime environmental factor that promoted the succession of the bacterial community. The diversity and structure of the bacterial community varied at different stages of the bioprocess, where BSFL induced Corynebacterium, Marinobacter, and Brevibacterium. EC: 4.2.1.17 (Enoyl-CoA hydratase) and EC: 1.1.1.35 (3-hydroxyacyl-CoA dehydrogenase) were the key lipid metabolic enzymes, promoting the degradation and transformation of materials and lipids. The synergistic effect of BSFL and microbiota promotes lipid metabolisms in DBW, which is conducive to the sustainable utilization of BSFL biotechnology to convert wastes into high-value resources.
The Spotted Lanternfly Contains High Concentrations of Plant Hormones in Its Salivary Glands: Implications in Host Plant Interactions
Authors: ACEVEDO FLOR E. 
Published: March 2024

Abstract

The spotted lanternfly (SLF), Lycorma delicatula is an invasive species in the United States that has emerged as a significant pest in vineyards. This polyphagous insect causes significant damage to grapevines and tree of heaven (TOH). SLF feeds voraciously on plant tissues using its piercing and sucking mouthparts through which it injects saliva and uptakes plant sap. Despite its impact, research on fundamental mechanisms mediating SLF interactions with their predominant hosts is limited. This study documents the morphology of salivary glands and quantifies plant hormones in salivary glands of SLF adults fed on grapevines and TOH using Liquid Chromatography-Mass Spectrometry (LC/MS). SLF adults have one pair of large salivary glands, ranging from 10–15 mm in length that extend from the insect’s head to the last sections of the abdomen. The salivary glands of SLF contain salicylic acid (89 ng/g), abscisic acid (6.5 ng/g), 12-oxo-phytodienoic acid (5.7 ng/g), indole-3-acetic acid (2 ng/g), jasmonic acid (0.6 ng/g), jasmonic acid isoleucine (0.037 ng/g), and the cytokinin ribosides trans-zeatin (0.6 ng/g) and cis-zeatin (0.1 ng/g). While the concentrations of these hormones were similar in insects fed on grapevines and TOH, abscisic acid was more abundant in insects fed on grapevines, and jasmonic acid isoleucine was only detected in insects fed on grape. These results are discussed in the context of the possible implications that these hormones may have on the regulation of plant defenses. This study contributes to our understanding of the composition of SLF saliva and its potential role in plant immunity.
Development of a Spatial Accuracy Compensation Method Based on Multi-Beam Laser Interferometer
Authors: Lei Lihua , Sun Changjian , Cao Chenming , Liang Lijie , Fu Yunxia 
Published: March 2024

Abstract
A mathematical model has been established for the error associated with the degree of freedom for each motion axis, with a correction applied based on the 12-line method in three-dimensional space. A multi-beam laser interferometer is proposed, which comprises four lasers, where different laser combinations can be used to measure different parameters, ultimately achieving simultaneous measurement of errors for the six degrees of freedom. This has informed the development of a spatial error measurement and accuracy compensation system. The error in the X/Y/Z axis data of the computer numerical control (CNC) machine tool is determined using the multi-beam laser interferometer. Spatial accuracy compensation is achieved by applying a software algorithm, and the positioning accuracy is obtained by measuring the same axis at different spatial coordinate positions to verify correction of the angular error. The experimental results have confirmed that after spatial error compensation, linear positioning accuracy, horizontal and vertical straightness errors improved by nearly 96%, 89% and 55% respectively, and the average and maximum deviations of positioning accuracy improved by nearly 88% and 91% respectively.
Dynamic changes in the real-time glomerular filtration rate and kidney injury markers in different acute kidney injury models
Authors: Xin Yu , Liu Yanqi , Liu Linqiong , Wang Xinran , Wang Dawei , song yuchen , shen lifeng , liu yuxi , liu yuhan , peng yahui , wang xibo , zhou yang , li hongxu , zhou yuxin , huang pengfei , yuan mengyao , xiao yu , yu kaijiang , wang changsong 
Published: March 2024

Abstract

In this study, the dynamic changes in the real-time glomerular filtration rate in acute kidney injury models were monitored via percutaneous glomerular filtration rate monitoring technology. Male C57BL/6 mice were used to establish sepsis, ischemia‒reperfusion, cisplatin, and antibiotic-induced acute kidney injury models. In addition to the real-time glomerular filtration rate, renal tissue NGAL and Kim-1 mRNA expression levels and serum creatinine and blood urea nitrogen levels were also used to evaluate renal function. The results showed that the changes in biomarkers always lagged the real-time glomerular filtration rate during the progression and recovery of renal injury. In sepsis-induced acute kidney injury, the glomerular filtration rate decreases significantly as early as 2 hours after modeling, similar to renal injury induced by ischemia‒reperfusion, and the glomerular filtration rate can still better reflect renal insufficiency in the recovery stage of injury. In a model of antibiotic-induced kidney injury, vancomycin plus piperacillin-tazobactam did not worsen nephrotoxicity.
Evaluating the effectiveness of Toxfin and Novasil as dietary aflatoxin-binding agents in broilers for sustaining hepatic antioxidant capacity and intestinal health status during aflatoxin B 1 exposure
Authors: Alqhtani Abdulmohsen , Sulaiman Ali Al , Abudabos Ala 
Published: March 2024

Abstract

To assess the efficacy of Toxfin and Novasil as aflatoxin-binding agents in broilers exposed to aflatoxin B1 (AFB1) from 11–30 d, 288 mixed-sex Ross 308 broiler chickens were randomly allocated to four dietary groups: control feed, control feed + 0.25 mg/kg AFB1, AFB1 feed + 0.3% Toxfin, and AFB1 feed + 0.3% Novasil. The evaluation encompassed growth performance for the grower (11–20 d), finisher (21–30 d), and overall (11–30 d) phases, carcass characteristics, serum biochemical components, liver function enzymes, hepatic antioxidant capacity, AFB1 residue in the liver and kidney, and ileal morphology at 30 d, and apparent nutrient digestibility during 29–30 d. Exposure to AFB1 significantly resulted in reduced growth efficiency, lowered carcass yields, liver hypertrophy, impaired metabolic and hepatic functions, liver oxidative stress, disrupted ileum architecture, diminished nutrient digestibility, and accumulated AFB1 in liver and kidney. Conversely, supplementation of Toxfin or Novasil significantly augmented body weight gain (BWG) and reduced feed conversion ratio (FCR) during the finisher and overall phases, elevated BWG in the grower phase, heightened levels of glucose, hepatic protein, and glutathione peroxidase, declined malondialdehyde content, improved apparent metabolizable energy, and lowered AFB1 residues in the liver and kidney. Furthermore, Toxfin inclusion significantly reduced FCR during the grower phase, enhanced European production efficiency factor during the grower and overall phases, augmented dressing percentage, declined proportional liver weight, elevated concentrations of total protein, albumin, and total antioxidant capacity, heightened villus surface area, and boosted crude protein digestibility. To conclude, incorporating 0.3% Toxfin into broilers' feeds confers a more effectual safeguard than Novasil against the deleterious consequences of AFB1 exposure.
Ulcerative colitis and Nephrotic syndrome: a two-sample Mendelian randomization study
Authors: Zhan Jinfeng , Rao Yujie , Liu Jiahao , Miao Haizhou , Xia Zhongbin 
Published: March 2024

Abstract

Objectives
This study employed Mendelian randomization to investigate the potential causal relationship between ulcerative colitis and nephrotic syndrome
Methods
We obtained the whole-genome association study data of ulcerative colitis in the European population from the GWAS database. Relevant single nucleotide polymorphisms (SNPs) were selected for analysis. We employed the inverse variance-weighted meta-analysis of multiplicative random effects models to obtain SNP-specific Wald ratio estimates, which assume horizontal pleiotropy. In addition, we performed sensitivity analyses using MR-Egger, weighted median, and IVW.
Results
Our findings suggest a strong association between ulcerative colitis and nephrotic syndrome(P < 0.05). After conducting sensitivity analyses, we found no evidence of horizontal pleiotropy or heterogeneity(P > 0.05).
Conclusion
Our Mendelian randomization study provides evidence for an association between ulcerative colitis and nephrotic syndrome, which may help us better understand the conditions of ulcerative colitis and nephropathy, as well as their relationship.
Evaluation of the Relationship Between Frailty Level and Quality of Life in Elderly Individuals with Type 2 Diabetes
Authors: RASHİDİ Mahruk , YILDIRIM Gülay , KARAMAN Funda , ÇAKMAK Sultan , DURUSOY Ebru , ŞAHİN Buse SAYGIN , GENÇ Aslı , AKGÖZ Hasan Fatih 
Published: March 2024

Abstract

Background
The aim of the study was to examine the relationship between frailty level and quality of life in elderly individuals with Type 2 diabetes.
Methods
This descriptive and correlational study was conducted with 211 patients who applied to the diabetes outpatient clinic of a private and a public hospital in Istanbul between September and November 2022. The data were obtained by using the Personal Information Form, Edmonton Frail Scale and the Quality of Life in the Elderly Scale.
Results
The mean Edmonton Frail Scale score of the participants was 7.36 ± 4.35. Their mean quality of life scale score was 19.32 ± 7.12. A low degree of negative correlation was found between Elderly Quality of Life Scale total and subscale scores and the Edmonton Frail Scale score (p < 0.05). Besides, there was a statistically significant difference between the Edmonton Frail Scale mean score and education level, history of falling in the last 1 year, using oral antidiabetic drugs, and the presence of diabetes complications (p < 0.05).
Conclusion
It is recommended that frailty and quality of life should be evaluated regularly in elderly patients with diabetes and necessary planning should be made in diabetes management and improving quality of life in the presence of frailty.
A Randomized, Multicenter, Treatment-as-usual Controlled Clinical Trial to Evaluate the Safety and Efficacy of Digital Cognitive Behavioral Therapy for Eating Disorders in South Korea: Study Protocol
Authors: Moon Daa Un , Lee Jung-Hyun , Song Youn Joo , Kim Kyung Ran , Kang Min Jae , Choi Soo-Hee , Lee Joongsuk , Lee Yujin , Kim Youngin , Jung Young-Chul 
Published: March 2024

Abstract

Background
Eating disorders, including anorexia nervosa, bulimia nervosa, and binge eating disorder, present significant challenges to both physical health and psychosocial functioning. Despite cognitive behavioral therapy (CBT) being an effective treatment for binge eating, accessibility issues limit its reach, underscoring the need for innovative treatment solutions. The digital cognitive behavioral therapy (dCBT) application "WELT-ED" presents a promising alternative, aiming to bridge this gap by offering accessible, scalable, and cost-effective treatment.
Methods
This study is a two-center, two-arm randomized controlled trial comparing the efficacy and safety of "WELT-ED" against standard care for individuals with eating disorders in South Korea. A total of 134 participants will be enrolled, randomly divided into either the dCBT intervention group (n = 67) or a control group receiving standard care (n = 67). Assessments at baseline, midpoint, and post-intervention will evaluate primary outcomes such as binge eating frequency, and secondary outcomes including psychosocial impairment. Safety, compliance, and satisfaction within the intervention group will also be analyzed to provide a comprehensive evaluation of the dCBT's impact.
Discussion
This research aims to investigate the possibility of "WELT-ED," a dCBT app, addressing the treatment gap for eating disorders in South Korea. By evaluating its efficacy, safety, and user satisfaction, the results could support incorporating digital interventions into standard care. This may transform how eating disorders are managed by improving access to evidence-based therapies.
Trial registration
This study was approved by the Institutional Review Board of Yonsei Severance Hospital (No. 2022-2865-002) and Seoul National University Hospital (No. D-2212-043-1385). It has been registered on ClinicalTrials.gov (27 February 2025, identifier: NCT06294353).
Location Based Collision Analysis to Detect Accident Blackspot Zones and Its Alternative Measures for Coimbatore City
Authors: Selvasofia S. D. Anitha , Jebaseelan S.D Sundarsingh 
Published: March 2024

Abstract
In this work, an effort has been made to identify the accident prone zones within Coimbatore district, Tamilnadu. For this purpose, the road accident data during the period pertaining to Coimbatore district have been used. Accident particulars like date, location, type of vehicle involved, number of persons injured or died were included in the GIS database. The objective of the work is to identify the accident prone areas on the national highways namely Rakkipalayam Junction, Anaikatti, Thudiyalur junction and Thanner panthal and to identify the high accident prone locations and safety deficient areas. The road geometry was measured in the accident prone locations to find out the deficiencies in the geometric design of highways. The model was validated with the accident data performance of the model was found to be excellent. In order to reduce the traffic congestion and accident spots, remedial measures was suggested. Suggestions were given to remove or relocate the bus stops and the new traffic signals were proposed. The emergence of traffic and subsequently traffic congestion in urban road networks are increasing worldwide with the growing number of vehicles, which results in excess delay, and reduced safety. Based on the GIS analysis, it was found that traffic volume is one of the major causes for the traffic congestion. Finally the percentage area of high risk zones and very high risk zones have reduced while the percentage area of medium traffic congestion zone have increased by 20.23 percentage and the percentage decreased in area of safe zone is 16.79%.
An effective study on the diagnosis of colon cancer with the developed local binary pattern method
Authors: Gül Mehmet 
Published: March 2024

Abstract

According to a recent study, 1 million people died from colon cancer and approximately 2 million from lung cancer. Regardless of the type of cancer, identifying the tumor area is extremely important. The pathology method is the most trustworthy technique for locating the tumor. Nucleus detection and classification studies were performed on images obtained with the pathology method. The principal objective of this study is to ascertain the presence of the tumor and acquire insights into its behavior. There could be some complications while the pathology procedure is performed. On the other hand, it is also important that the samples obtained are examined correctly by experts. Within the scope of the study, the local binary pattern method was used as a highly effective method among image enhancement methods. Colon cancer was diagnosed with two valuable local binary pattern (LBP) methods derived from the local binary pattern (LBP) method. During the diagnosis procedure, the developed LBP methods were first evaluated with machine learning and some transfer learning (TL) methods. Within the scope of the study, the LC25000 dataset was used to analyze colon cancer histopathological images. The performance values for step LBP method analysis were, respectively, accuracy (96.87%), kappa (93.74%), precision (96.9%), recall (96.9%), F1 score (96.9%), and ROC (99.4%). The results obtained with the developed cross-over LBP method were, respectively, accuracy (94.57%), kappa (90.91%), precision (94.9%), recall (94.9%), F1 score (94.9%), and ROC (98.8%).
Secure Cloud Data with Attribute-based Honey Encryption
Authors: Siyal Reshma , Long Jun 
Published: March 2024

Abstract
Encryption is a Technique to convert plain text into Cipher text, which is unreadable without an appropriate decryption key. Hadoop is a platform to process and store huge amounts of data reliably. It also provides a flexible service for big data through HDFS storage. Inappropriately, Hadoop's lack of built-in security measures makes it more likely that hostile attacks will be made against the data processed or stored using Hadoop. So, the data stored in Hadoop is a big, challenging task. The prevalence of private data attacks underscores the critical need for robust Encryption techniques. In this article, we have used Attribute-based encryption based on Cipher text policy attributes encryption. Attribute-based Honey Encryption (ABHE) is a novel encryption technique that provides an additional layer of security to data stored in a database. This paper especially focused on the file sizes encoded inside the Hadoop. Hadoop is good for handling big data but it lacks security, making data vulnerable to attacks. To fix this, we have created Attribute Honey Encryption (ABHE), a method to encode and decode files securely in HDFS, and compared work with the CP-ABE algorithm and KP-ABE algorithm. ABHE works well even with large files and performs better. This makes Hadoop safer for storing and processing data.
Rural Tourism Combined with Relaxation Guidance on Fertility Outcomes in the Infertility Population
Authors: fu xianchun , zhong di , Dai Zhijun , Ni Gang , Hong Mingyun 
Published: March 2024

Abstract
This study aims to investigate the effects of rural tourism combined with relaxation guidance on the conception rates and live birth rates among patients suffering from infertility.A retrospective cohort study was conducted, involving 208 infertile couples who received treatment at a reproductive centre in Anhui province between September 2021 and September 2023. The primary indicators monitored in this study were the pregnancy rate, live birth rate, and emotional state of the patients.Out of the participants, 32% had recently undergone rural tourism combined with relaxation guidance. The study found that those who received this combined approach had a significantly higher conception rate (88% vs. 54%) and live birth rate (71% vs. 37%) compared to those who did not participate in the rural tourism and relaxation guidance.The findings suggest that incorporating rural tourism with relaxation guidance significantly improves the live birth rates in infertile patients. Therefore, it is recommended that healthcare providers consider this combined approach as a part of the infertility treatment and care plan for patients.