bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a Commercially Available Serum Competitive ELISA for Detection of Specific Antibodies to <em>Neospora caninum</em> in Raw Milk of Ruminants
Authors: Fereig Ragab , Altwaim Sarah A. , Frey Caroline F. 
Published: March 2024

Neosporosis is an infection caused by the protozoan parasite Neospora caninum and has substantial veterinary hazards. Neosporosis cannot be controlled via vaccination or a chemotherapeutic agent. Thus, accurate diagnosis followed by isolation and culling of infected animals is regarded as the most efficient way of control. In vivo diagnosis often relies on serologic testing of the animals, and milk represents a non-invasive and easy to collect sample matrix. However, ELISAs specifically designed for the antibody detection in milk are sometimes not easily available and it is tempting to use ELISA kits designed for use in serum also for milk samples. Herein, we evaluated a widely used commercial ELISA (ID Screen® Neospora caninum competition Multispecies ELISA (ID. Vet, Grabels, France)), developed for detection of N. caninum antibodies in serum samples, for its performance with milk samples instead. Milk samples from dairy ruminants (cows, buffaloes, sheep, goats; n = 149) were tested in parallel with the serum ELISA and a commercial milk ELISA as a standard test (Neospora caninum Milk Competitive ELISA, ID. Vet, Grabels, France). The detected prevalence rates were 42 (28.2%), 26 (17.4%), and 26 (17.4%) using milk ELISA, serum ELISA, and both ELISAs, respectively. Sensitivity, specificity, positive predictive value, and negative predictive value for the serum ELISA used with milk samples were 61.9%, 100%, 100%, 87%, respectively: Agreement and Kappa value between the two ELISAs were 89.3%, and 0.70, respectively. High values of Pearson correlation coefficient (0.991) and area under ROC curve (0.789) demonstrated a high diagnostic performance of serum ELISA also in milk samples. Also, Bland-Altman plot and Histogram describing the frequency of distribution of ELISA optical densities confirmed the high agreement of both serum and milk ELISAs. The current results revealed a high specificity but moderate sensitivity of the serum ELISA used for milk samples compared with the milk ELISA. However, the excellent positive predictive value of the serum ELISA makes it an alternative option in case of unavailability of milk ELISA. With this study, we provided additional evidence that a widely used serum ELISA test kit may also be used for detection of N. caninum antibodies in milk samples.
Implementasi Asset Based Community Development Dalam Menumbuhkan Modal Sosial, Ekonomi dan Budaya Pada Masyarakat Pesisir Desa Branta
Authors: Bagas M. Chindra , Sholikhah Riska Alfiyah , Faroha Siti , Rahmawati Vina 
Published: March 2024

<span>Penelitian ini bertujuan untuk mengungkap Implementasi Asset Based Community Development Dalam Menumbuhkan Modal Sosial, Ekonomi dan Budaya Pada Masyarakat Pesisir Desa Branta. Penelitian tentang Implementasi Asset Based Community Development Dalam Menumbuhkan Modal Sosial, Ekonomi dan Budaya Pada Masyarakat Pesisir ini dilakukan dengan menggunakan pendekatan kulitatif dengan jenis penelitian lapangan (field research) dengan teknik pengumpulan data menggunakan Teknik Wawancara (Interview), Observasi (Pengamatan) dan Dokumentasi. Adapusn subyek penelitian atau informan dalam peneliti ini adalah Kepala Desa dan Tokoh Masyarakat Desa Branta. Hasil penelitian menunjukkan bahwa Pengembangan aset dengan pendekatan ABCD telah diterapkan disalah satu desa pesisir Pulau Madura yakni desa Branta Pesisir. Kekayaan alam yang melimpah diiringi dengan keuletan masyarakatnya sehingga menjadikan desa Branta Pesisir sebagai salah satu desa yang maju dalam bidang perekonomiannya Salah satu pendobrak perekonomian Desa Branta Pesisir yaitu sektor UMKM, UMKM di Desa ini sangat beragam mulai dari UMKM olahan ikan, ikan kering dan lain sebagainya. Dengan demikian, hasil dari penelitian yang telah dilakukan menjelaskan bahwa aset yang ada di desa Branta Pesisir menjadi sarana pengembangan kesejahteraan masyarakat meliputi aset manusia, aset alam, aset ekonomi, aset infrastruktur, aset sosial dan budaya setempat. Adapun proses dalam pengembangan aset tersebut terbagi menjadi 5 (lima) tahapan yakni discovery (pengkajian), dream (impian), design (prosedur atau perencanaan), define (aksi) dan destiny (evaluasi atau monitoring).</span>
Paradigm of integration-interconnection: M. Amin Abdullah and Mehdi Golshani's perspective
Authors: Fian Khafifatul , Hidayat Fahri 
Published: March 2024

<p><span lang="EN-US">The existence of a monodisciplinary approach leaves no room for discussion between the sciences. There needs to be an interconnection and integration approach so that some sciences can reveal ideas to solve the problems faced. The focus of this research is on the comparison of the thoughts of M. Amin Abdullah and Mehdi Golshani on the integration of scientific interconnections. This research is library research through data collection techniques by searching for sources, either articles, books, or other sources relevant to the topic of discussion. After collecting the data, it is selected, analyzed, and conclusions formulated. The results showed that Mehdi Golshani supported the integration of scientific interconnection, but only for religion and science. In contrast to the perspective of M. Amin Abdullah, who created the idea of interconnection between religious science and general science, not only science was described through spider webs. M. Amin Abdullah assumes that religion and general science must be open to each other, even though each science has characteristics. With openness, it can create space for discussion so that what is debated as a problem can be criticized to produce a more comprehensive opinion.</span><span lang="EN-US"> </span></p>
Pustakawan di Ruang Virtual: Menggali Potensi dan Tantangan dalam Melayani Pengguna Melalui Platform Digital
Authors: YUSUF M. , Oktabrilianto Catur , Suhendro Prastomo 
Published: March 2024

<span lang="IN">Penelitian ini bertujuan untuk menjelajahi peran pustakawan dalam melayani pengguna melalui platform digital dan mengidentifikasi potensi dan tantangan yang terkait. Dalam latar belakangnya, perpustakaan semakin mengadopsi ruang virtual sebagai alternatif untuk memberikan layanan kepada pengguna dalam era teknologi informasi yang berkembang pesat. Metode penelitian yang digunakan adalah studi literer, dengan mengumpulkan dan menganalisis artikel, buku, dan sumber-sumber terkait lainnya. Hasil penelitian menunjukkan bahwa pustakawan memiliki potensi besar dalam melayani pengguna melalui platform digital. Mereka dapat memberikan akses mudah ke koleksi digital, memberikan bantuan dan panduan secara online, serta berkolaborasi dengan pengguna secara virtual. Namun, terdapat tantangan yang harus diatasi, seperti kesenjangan akses teknologi, kendala teknis dan keamanan, serta tantangan komunikasi dan interaksi dalam lingkungan virtual. Penelitian ini memberikan pemahaman yang lebih dalam tentang peran pustakawan dalam ruang virtual dan menyoroti potensi serta tantangan yang perlu diperhatikan. Dengan pemahaman ini, pengembangan layanan perpustakaan dalam ruang virtual dapat lebih efektif. </span>
Intestinally Targeted Glipizide Delivery via pH-responsive Dextran-Modified Polylactic acid Microspheres.
Authors: Kumar Sumit , Singh Ankita , Pandey Kalpana , Bansal Smriti , Sarkar Anjana , Poddar Deepak , Yoo Hyeongmin 
Published: March 2024

Abstract
Glipizide, a low-cost antidiabetic drug, is known for its fast-acting nature and reduced risk of hypoglycemia. Its non-selectivity and short-acting nature, however, restrict its use. In this study, the core-shell particle of glycidyl trimethyl ammonium chloride modified dextran-coated glipizide polylactic acid has been fabricated capable of targeted drug release in the intestinal region with acidic pH resistivity and mucoadhesive properties. To achieve this glipizide-encapsulated polylactic acid (PLA), microspheres of size 27.09 ± 6.55 µm were fabricated using emulsion solvent evaporation followed by GT-dextran surface coating using the dipping method. NMR and FT-IR confirmed the presence of GT-dextran, and SEM confirmed the presence of a coating on the surface of PLA particles. The GT-dextran coating increases the size of the GI-PLA particles by 14.01%, with a size of 17.17 ±1.33 µm, along with 57.47 ± 2.7% encapsulation efficiency. The release behavior showed that the particle was slowly released at 8.76 ± 0.93% at an acidic pH of 1.5 and normally released at 92.4 ± 7.32% at a pH of 7.2. This shows that the particle is resistant to acid. The desired controlled release profile was achieved, with 68.45 ± 11.01% at pH 9.2 over a time of 48 h and best fits the Korsmeyer-Peppas model and follows zero-order kinetics, which exhibited great potential as a targeted and controlled drug delivery system for antidiabetic therapy.
Rehabilitation outcomes after comprehensive post-acute inpatient rehabilitation following moderate to severe acquired brain injury – study protocol for an overall prognosis study based on routinely collected health record data
Authors: Pommerich Uwe M. , Stubbs Peter W. , Nielsen Jørgen Feldbæk 
Published: March 2024

Abstract

Background
The initial theme of the PROGRESS framework for prognosis research is termed overall prognosis research. Its aim is to describe the most likely course of health conditions in the context of current care. These average group level prognoses may be used to inform patients, health policies, trial designs or further prognosis research. Acquired brain injury, such as stroke, traumatic brain injuries or encephalopathy, is a major cause of disability and functional limitations, worldwide. Nevertheless, rehabilitation, aiming at maximizing independent functioning and meaningful participation in society post-injury, is considered both effective and cost-effective. While some observational studies, in theory, allow an inference of the overall prognosis of the level of independent functioning, the context for the provision of rehabilitation is often only sparsely described. The aim of this protocol is to provide a detailed account of the clinical context to aid the interpretation of our upcoming overall prognosis study.
Methods
The study will take place at a Danish post-acute inpatient rehabilitation facility providing specialised inpatient rehabilitation for individuals with moderate to severe acquired brain injury. Routinely collected electronic health record data will be extracted from the healthcare provider's database and deterministically linked on an individual level to construct the study cohort. The study period spans from March 2011 to December 2022. Four outcome measure for the level of functioning and rehabilitation needs will be described, both for the entire cohort, across rehabilitation complexity levels and stratified for relevant demographic and clinical parameters. Descriptive statistics will be used to estimate average prognoses for the level of functioning at discharge from post-acute rehabilitation. The patterns of missing data will be investigated.
Discussion
As the estimates will be based on routinely collected clinical data we deem the present account of particular importance. This protocol is intended provide transparency in our upcoming study, thus aiding the readers to interpret the overall prognosis estimates within the context of our current clinical practice and assess potential sources of bias independently.
A qualitative study on the factors influencing role transition in elderly patients with acute myocardial infarction preparing for discharge
Authors: Zhang Jie , Qiang ChunLing , Su LiQing , Li MengXue , Zhang Yumeng , Zheng ZiYi , Sun WeiJin , Chang Jian 
Published: March 2024

Abstract

Background Elderly patients with acute myocardial infarction find it difficult to restore their heart function to a normal level, and they often live with the disease for a long time, greatly impacting their quality of life and health conditions .Methods From September 2022 to June 2023, a phenomenological research method will be employed. Semi-structured in-depth interviews will be conducted with 4 doctors, 4 nurses, 9 patients, and 4 family members from a tertiary hospital in Shanghai. The interview data will be transcribed, coded, analyzed, and themes will be extracted.Results The factors influencing role transition in elderly patients with acute myocardial infarction during their discharge preparation can be summarized into four main themes.On the patient side, these include negative issues associated with aging, negative emotions, lack of role behaviors, and reinforcement of role behaviors. On the family side, the factors include a lack of economic support, caregiver knowledge deficiency, difficulties in adapting to the caregiver role, and busy work schedules of family members. Environmental factors encompass difficulties in adapting to the hospital environment, changes in comfort level, and inadequate discharge services provided by the hospital. Lastly, social factors include the availability of well-equipped community hospitals, supportive friendships and neighborly relationships, a lack of coordination between hospitals and the community, and inconvenience in seeking medical treatment in a different location.Conclusion The role transition experienced by elderly patients with acute myocardial infarction during their discharge preparation is influenced by various factors. It is important to increase awareness of this issue at various levels of society and provide support to help patients adjust their emotions and adapt to the transition from a healthy role to a role affected by illness. Encouraging patients to actively cope with the disease and adapt to all aspects of change, as well as involving them in disease management, is crucial. Improving the caregiving abilities of healthcare professionals, strengthening the continuity of long-term follow-up, developing service systems, and implementing personalized intervention measures that are suitable for the clinical scenarios in China are essential steps.
Examining the Role of Negative Attentional Bias in Patients with Adolescent Depressive Disorders: An Emotion-Attention Blink Task Study.
Authors: Zhu Shasha , Zhao Yuqing , Lu Hehe , Guo Feng , Lin Chunting , Qiu HuiLin , Jiang Ke 
Published: March 2024

Abstract
Background
Negative cognitive bias significantly influences the onset, progression, and persistence of depression. However, the detailed aspects of attentional bias within this negative cognitive framework remain insufficiently delineated. This study aimed to (1) employ the attentional blink disengagement effect for assessing cognitive bias in facial emotion recognition among individuals with depressive disorders and (2) explore the specific elements of negative bias in these patients.
Methods
Thirty-three adolescents diagnosed with depressive disorders and 33 control subjects were selected based on the Depressive Disorder Severity Questionnaire (DDSQ) and psychiatric interviews. A mixed-design emotion-attention blink task (4 SOAs: 0 ms/235 ms/706 ms/1176 ms × 2 tasks: dual task/single task × 2 subgroups: control group/depressive disorder group) was utilized to measure the accuracy of emotion recognition. Differences in negative attentional bias between the two groups were analyzed using repeated-measures ANOVA.
Results
Adolescents with depressive disorders exhibited a negative attentional bias, primarily characterized by difficulties in attentional disengagement. The disparity in this negative attentional bias was most pronounced in scenarios involving exclusively negative emotional faces. Clinical trial registration number ChiCTR2300067671.
Conclusion
Negative attentional bias is a hallmark of processing styles in patients with depressive disorders. In adolescent patients, this bias in emotion recognition tasks predominantly manifests as difficulties in attentional disengagement.
The Drivers of 90-Day CABG Episode Payment Variation for Commercially Insured Patients
Authors: Alsarhan Saleh , Delgado Rigoberto I. , Krause Trudy Millard , Aguilar David , Mikhail Osama 
Published: March 2024

Abstract
Coronary artery bypass grafting (CABG) surgery has become a target for episode payment initiatives, and there is the need to understand what factors contribute to costs of CABG surgeries. 90-day episode payments for CABG surgery were estimated using commercial insurance claims of patients over 18 years of age in Texas. The study used a multiple linear regression model with a log-transformed 90-day CABG episode payments to model effect of patient factors on payment variation. The source of data was Optum’s de-identified Clinformatics® Data Mart Database (CDM): administrative claims data. A total of 999 CABG episodes were identified. The mean (SD) 90-day CABG episode payment per patient was $81,330 ($47,382). Patient factors explained about 37% of the payment variation. Wide variation exists in 90-day CABG episode payments for commercially insured patients across Texas. Focusing on reducing variation of index hospitalization could be a potentially effective approach to improve efficiency.
Identification of hub genes related to Alzheimer's disease by bioinformatics analysis and observation of the pathological characteristics
Authors: Wang Jinping , Qu Yidi , Huang Yuhua , Zeng Hanlin , Chui Enshan , Yao Chun 
Published: March 2024

Abstract

Objectives: Alzheimer’s disease (AD) is a neurodegenerative disease that often occurs in the elderly population, with complex pathogenesis. The aim of this paper is to explore related indicators in AD.
Methods: Two microarray datasets associated with ‘Alzheimer’s disease’ were screened and the common differentially expressed genes (DEGs) were determined by GEO2R. GO and KEGG pathway analysis of DEGs was performed by DAVID. Hub genes were screened by protein-protein interaction (PPI) network. The AD mouse model was constructed and HE staining was carried out to observe the pathological characteristics of brain tissues. The expression of oxidative stress related indicators in serum and hub genes in tissues were detected by ELISA and qRT-PCR, respectively.
Results: There were 259 common DEGs in the GSE1297 and the GSE5281 datasets. Six hub genes, ACTB, H3C12, CREBBP, CXCR4, NFKBIA, and SNAP25, were selected by PPI network. HE staining demonstrated that the hippocampal region in the AD group showed obvious atrophy, and degeneration and necrosis of scattered or continuous neurons could be seen. ELISA results showed that the contents of SOD and GSH-px in the serum of the AD group were significantly reduced, with increased MDA.
Conclusion: Hub genes involved in the development of AD were identified by bioinformatics analysis, including ACTB, H3C12, CREBBP, CXCR4, NFKBIA, and SNAP25. Oxidative stress is associated with AD development.
Calculation of Windshear from Radiosonde Observations in Saudi Arabia
Authors: Zamreeq Arjan O. , Hasanean H. M. 
Published: March 2024

Abstract

The windshear (WS) or wind gradient is a short-lived microscale or meso-gamma weather phenomenon and is potentially very dangerous for aviation everywhere. It is commonly observed near a microburst or downburst caused by different factors including thunderstorms and may increase in the era of climate change. This research presents the calculation of WS frequency and intensity from radiosonde data at six stations in Saudi Arabia for the period 1991-2020. The radiosonde data were used to calculate light, moderate, strong, and severe WS frequency and intensity at Abha, Jeddah, Madinah, Tabuk, Riyadh, and Dammam airport stations. The frequency and intensity of WS calculated from radiosonde data from 0-1200 m height indicate that the number of severe WS increased suddenly from 2015 below 30 m level at all stations except for Riyadh there is no sudden increase. At all other levels, the number of light WS increased from bottom to up to ~ 900 m and then gradually decreased at 1200 m irrespective of stations. Overall, the highest WS observed in the autumn season for all stations except it is in summer for Jeddah. The occurrence of WS is higher in the daytime as compared to the nighttime round the year for Dammam, Madinah, Riyad, and Tabuk while the pattern is opposite for Jeddah. In Abha, it depends on the season. The sudden increase of severe WS from 2015 may be linked to the rise of surface pressure, temperature, and wind speed at station levels or they may occur due to outflow from the airplane landing. However, further investigation is required to understand the large number of severe WS and its relation to climate change in the region along with teleconnection to the large-scale circulations.
Scaling up noncommunicable disease care in a resource-limited context: lessons learned and implications for policy
Authors: Mamo Yoseph , Mekoro Mirchaye , Phillips David , Mortimore Andrew 
Published: March 2024

Abstract

Background:Although primary care models for the care of common noncommunicable diseases (NCD) have been developed in sub-Saharan Africa, few have described an integrated, decentralized approach at the community level. We report the results of a four-year, Ethiopian project to expand this model of NCD care to 15 primary hospitals and 45 health centres encompassing a wide geographical spread and serving a population of approximately 7.5 million people.Methods:Following baseline assessment of the 60 sites, 30 master trainers were used to cascade train a total of 621 health workers in the diagnosis, management and health education of the major common NCDs identified in a scoping review (hypertension, diabetes, chronic respiratory disease and epilepsy). Pre- and post-training assessments and regular mentoring visits were carried out to assess progress and remedy supply or equipment shortages and establish reporting systems. The project was accompanied by a series of community engagement activities to raise awareness and improve health seeking behaviour.Results:A total of 643,296 people were screened for hypertension and diabetes leading to a new diagnosis in 24,313 who were started on treatment. Significant numbers of new cases of respiratory disease (3,986) and epilepsy (1,925) were also started on treatment. Mortality rates were low except for hypertension in the rural health centres where 311 (10.2%) died during the course of the project. Loss to follow up (LTFU), defined as failure to attend clinic for >6 months despite reminders, was low in the hospitals but represented a significant problem in the urban and rural health centres with up to 20 to 30 % of patients with hypertension or diabetes absenting from treatment by the end of the project. Estimates of the population disease burden enrolled within the project, however, were disappointing; asthma (0.49%), hypertension (1.7%), epilepsy (3.3%) and diabetes (3.4%).Conclusion:This project demonstrates the feasibility of scaling up integrated NCD services in a variety of locations, with modest cost and methodology that is replicable and highly sustainable. However, the relatively small gain in the detection and treatment of common NCDs highlights the huge challenge in making NCD services available to all.