bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross‐linking chemistry and biology: development of multifunctional photoaffinity probes
Authors: Tomohiro Takenori , Hashimoto Makoto , Hatanaka Yasumaru 
Published: November 2005

AbstractAn efficient method of photoaffinity labeling has been developed based on rationally designed multifunctional photoprobes. Photoaffinity techniques have been used to elucidate the protein structure at the interface of biomolecules by the photochemical labeling of interacting sites. However, the identification of labeled sites within target proteins is often difficult. Novel biotinyl bioprobes bearing a diazirine photophore have contributed significantly to the rapid elucidation of ligand binding sites within proteins, thereby extending conventional photoaffinity methods. This article discusses the synthesis and applications of various photoprobes bearing a biotin, including strategies using cleavable linkages between photophores. The combination of photoaffinity methods with chip technology is also described as a novel entry to rapid affinity‐based screening of inhibitors. This review focuses on a rapid and reliable photoaffinity method utilizing diazirine‐based multifunctional photoprobes with numerous potential applications in functional proteomics of biomolecular interactions. © 2005 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 5: 385–395; 2005: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20058
Discovery and development of microporous metal carboxylates
Authors: Mori Wasuke , Sato Tomohiko , Kato Chika Nozaki , Takei Tohru , Ohmura Tetsushi 
Published: November 2005

AbstractWe have found a form of copper(II) terephthalate that occluded an enormous amount of gases such as N2, Ar, O2, and Xe. Copper(II) terephthalate is the first metal complex found capable of adsorbing gases. This complex has opened a new field of adsorbent chemistry and is recognized as a leader in the construction of microporous metal complexes. In extending the route for the synthesis of microporous complexes, we obtained many new porous materials that are widely recognized as useful materials for applications in areas such as gas storage, molecular sieves, catalysis, inclusion complexes, and surface science. © 2005 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Chem Rec 5: 336–351; 2005: Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.20060
Routine pre‐evacuation ultrasound diagnosis of hydatidiform mole: experience of more than 1000 cases from a regional referral center
Authors: Fowler D. J. , Lindsay I. , Seckl M. J. , Sebire N. J. 
Published: November 2005

AbstractObjectivesTo examine the accuracy of sonographic findings of routine ultrasound examinations in patients with a proven histological diagnosis of complete or partial hydatidiform mole referred to a supra‐regional referral center, and to examine the relationship of sonographic findings to gestational age across the first and early second trimesters.MethodsReview of consecutive cases referred to a trophoblastic disease unit from June 2002 to January 2005 with a diagnosis of possible or probable hydatidiform mole in whom results of a pre‐evacuation ultrasound examination were documented. Ultrasound detection rates for partial and complete hydatidiform moles were calculated and comparison of detection rates between complete and partial mole, and gestational age groups carried out.Results1053 consecutive cases were examined. The median maternal age was 31 (range, 15–54) years and the median gestational age was 10 (range, 5–27) weeks. 859 had a final review diagnosis of partial or complete hydatidiform mole (82%), including 253 (29%) complete moles and 606 (71%) partial moles. Non‐molar hydropic miscarriage was diagnosed following histological review in 194 (18%). Overall, 378 (44%) cases with a final diagnosis of complete or partial hydatidiform mole had a pre‐evacuation ultrasound diagnosis suggesting hydatidiform mole, including 200 complete moles and 178 partial moles, representing 79% and 29%, respectively, of those with complete (253) or partial (606) moles in the final review diagnosis. The ultrasound detection rate was significantly better for complete versus partial hydatidiform moles (Z = 13.4, P < 0.001). There was a non‐significant trend towards improved ultrasound detection rate with increasing gestational age, with an overall detection rate of 35–40% before 14 weeks' gestation compared to around 60% after this gestation. The sensitivity, specificity, positive predictive value and negative predictive value for routine pre‐evacuation ultrasound examination for detection of hydatidiform mole of any type were 44%, 74%, 88% and 23%, respectively.ConclusionsRoutine pre‐evacuation ultrasound examination identifies less than 50% of hydatidiform moles, the majority sonographically appearing as missed or incomplete miscarriage. Detection rates are, however, higher for complete compared to partial moles, and improve after 14 weeks' gestation. Histopathological examination of products of conception remains the current gold standard for the identification of gestational trophoblastic neoplasia. Copyright © 2005 ISUOG. Published by John Wiley & Sons, Ltd.
Prenatal prognosis of congenital diaphragmatic hernia using magnetic resonance imaging measurement of fetal lung volume
Authors: Gorincour G. , Bouvenot J. , Mourot M.G. , Sonigo P. , Chaumoitre K. , Garel C. , Guibaud L. , Rypens F. , Avni F. , Cassart M. , Maugey‐Laulom B. , Bourlière‐Najean B. , Brunelle F. , Durand C. , Eurin D. 
Published: November 2005

AbstractObjectivesTo investigate the correlation between fetal lung volume (FLV), measured with magnetic resonance imaging (MRI), and postnatal mortality in newborns with prenatally diagnosed isolated congenital diaphragmatic hernia (CDH).MethodsIn a 4‐year prospective multicenter study, 77 fetuses with isolated CDH diagnosed between 20 and 33 weeks' gestation underwent fast spin‐echo T2‐weighted lung MRI. These MRI‐FLV measurements were compared with a previously published normative curve obtained in 215 fetuses without thoracoabdominal malformations and with normal ultrasound biometric findings. FLV measurements were correlated with postnatal survival. The mean gestational age at MRI was 31.3 weeks.ResultsThe measured/expected FLV ratio was significantly lower in the newborns with CDH who died compared with those who survived (23.6 ± 12.2 vs. 36.1 ± 13.0, P < 0.001). When the ratio was below 25%, there was a significant decrease in postnatal survival (19% vs. 40.3%, P = 0.008). Survival was significantly lower for neonates when one lung could not be seen by fetal MRI compared with those fetuses with two visible lungs on MRI (17.9% vs. 62.1%, P < 0.001).ConclusionIn isolated CDH, FLV measurement by MRI is a good predictor of postnatal mortality due to pulmonary hypoplasia. Copyright © 2005 ISUOG. Published by John Wiley & Sons, Ltd.
Fetal transesophageal echocardiography: clinical introduction as a monitoring tool during cardiac intervention in a human fetus
Authors: Kohl T. , Müller A. , Tchatcheva K. , Achenbach S. , Gembruch U. 
Published: November 2005

AbstractBecause of insufficient imaging by maternal transabdominal fetal echocardiography (TAE) in a human fetus with aortic atresia, imperforate atrial septum and progressive cardiac failure, we assessed the feasibility of fetal transesophageal echocardiography (TEE) as a monitoring tool during fetal cardiac intervention at 24 + 6 weeks of gestation. Percutaneous fetoscopic intraesophageal deployment of the ultrasound catheter was achieved and did not result in any maternal or fetal complications. Fetal TEE permitted substantially clearer definition of fetal cardiac anatomy and intracardiac device manipulations than conventional maternal TAE. Despite the employment of various devices, no sufficiently large opening could be achieved within the atrial septum. Although the fetus tolerated the procedure remarkably well and satisfactory fetoplacental flow could be documented at the end of the procedure, the fetus died from progressive cardiac failure 3 days after the intervention. Fetoscopic TEE is feasible in the human fetus and permits substantially clearer definition of fetal cardiac anatomy and intracardiac manipulations than conventional maternal TAE. Based on the observation of spontaneous closure of multiple iatrogenic perforations of the atrial septum, specialized devices are required in order to improve the technical success rate of septoplasty methods and hence the survival odds of these high‐risk patients. Copyright © 2005 ISUOG. Published by John Wiley & Sons, Ltd.
Evaluation of endometrial receptivity during in‐vitro fertilization using three‐dimensional power Doppler ultrasound
Authors: Järvelä I. Y. , Sladkevicius P. , Kelly S. , Ojha K. , Campbell S. , Nargund G. 
Published: November 2005

AbstractObjectiveTo compare sonographic endometrial characteristics in in‐vitro fertilization (IVF) cycles between women who conceive and those who do not.MethodsThirty‐five women undergoing IVF treatment participated in the study. Using three‐dimensional (3D) power Doppler ultrasound, we assessed endometrial patterns, volume and vascularization, after follicle stimulating hormone (FSH) stimulation but before human chorionic gonadotropin (hCG) administration (referred to hereafter as ‘after FSH stimulation’) and again on the day of oocyte retrieval.ResultsThe pregnancy rate was 37% (13/35). After FSH stimulation, 29 of the 35 women had a triple‐line endometrial pattern, compared with five out of 35 on the day of oocyte retrieval. In those who had a triple‐line pattern after FSH stimulation the pregnancy rate was 44.8% (13/29) and it was 0% (0/6) in those with a homogeneous pattern (chi‐square test, P = 0.039). If a triple‐line pattern was present on the day of oocyte retrieval the pregnancy rate was 80.0% (4/5), whereas if the pattern was homogeneous the pregnancy rate was 30.0% (9/30) (P = 0.032). There were no differences between those who conceived and those who did not in endometrial thickness, volume or vascularization on either day examined. Endometrial volume decreased significantly after hCG injection in women who conceived, but not in those who did not conceive. In both groups endometrial and subendometrial vascularization decreased after hCG injection, while the endometrial thickness remained unchanged.ConclusionsThe existence of a homogeneous endometrial pattern after FSH stimulation seems to be a prognostic sign of an adverse outcome in IVF, while a triple‐line pattern after FSH stimulation and a decrease in endometrial volume appear to be associated with conception. Copyright © 2005 ISUOG. Published by John Wiley & Sons, Ltd.
Number of yolk sacs does not predict amnionicity in early first‐trimester monochorionic multiple gestations
Authors: Shen O. , Samueloff A. , Beller U. , Rabinowitz R. 
Published: November 2005

AbstractObjectiveTo determine the relationship between amnionicity and number of yolk sacs before 11 weeks of gestation.MethodsTwenty‐two cases of monochorionic multiple pregnancy were scanned before 11 weeks of gestation. There were 21 sets of twins and one of triplets. Amnionicity was determined by visualization of a dividing amniotic membrane between the gestational sacs. The number of yolk sacs was recorded and compared with the presence or absence of a dividing membrane for all fetuses.ResultsIn 17/20 (85%) cases of monochorionic diamniotic twins, two yolk sacs were seen. In 3/20 (15%) cases of monochorionic diamniotic twins, a single yolk sac was seen. In the one case of monochorionic diamniotic triplets, two yolk sacs were visualized. In one case of monoamniotic twins, a single yolk sac was observed.ConclusionsIn monochorionic pregnancies, the presence of two yolk sacs predicts diamnionicity. However, the use of the number of yolk sacs as a predictor of amnionicity may not be accurate in a small proportion of patients. The diagnosis of monoamnionicity can be made only following a careful search for a dividing amniotic membrane. Copyright © 2005 ISUOG. Published by John Wiley & Sons, Ltd.
Determination of the properties of a polycapillary x‐ray lens
Authors: Sun Tianxi , Ding Xunliang 
Published: November 2005

AbstractThe energy dependence of the focal spot size, transmission efficiency and output focal distance of a polycapillary x‐ray lens was measured efficiently over a broad energy range. An axial scanning method was devised to determine the energy dependence of the output focal distance. The halo effect was studied by measuring the spatial distribution of x‐rays with various energies in the beam focused by the polycapillary x‐ray lens. Copyright © 2005 John Wiley & Sons, Ltd.
New weakly expressed cell cycle‐regulated genes in yeast
Authors: de Lichtenberg Ulrik , Wernersson Rasmus , Jensen Thomas Skøt , Nielsen Henrik Bjørn , Fausbøll Anders , Schmidt Peer , Hansen Flemming Bryde , Knudsen Steen , Brunak Søren 
Published: November 2005

AbstractWe present an approach combining bioinformatics prediction with experimental microarray validation to identify new cell cycle‐regulated genes inSaccharomyces cerevisiae. We identify in the order of 100 new cell cycle‐regulated genes and show by independent data that these genes in general tend to be more weakly expressed than the genes identified hitherto. Among the genes not previously suggested to be periodically expressed we find genes linked to DNA repair, cell size monitoring and transcriptional control, as well as a number of genes of unknown function. Several of the gene products are believed to be phosphorylated by Cdc28. For many of these new genes, homologues exist inSchizosaccharomyces pombeandHomo sapiensfor which the expression also varies with cell cycle progression. Copyright © 2005 John Wiley & Sons, Ltd.
Expression of the GPD1 and GPP2 orthologues and glycerol retention during growth of Debaryomyces hansenii at high NaCl concentrations
Authors: Gori Klaus , Mortensen Henrik D. , Arneborg Nils , Jespersen Lene 
Published: November 2005

AbstractThe highly NaCl‐tolerant yeast Debaryomyces hansenii produces and obtains high levels of intracellular glycerol as a compatible solute when grown at high NaCl concentrations. The effect of high NaCl concentrations (4%, 8% and 12% w/v) on the glycerol production and the levels of intra‐ and extracellular glycerol was determined for two D. hansenii strains with different NaCl tolerance and compared to one strain of the moderately NaCl‐tolerant yeast Saccharomyces cerevisiae. Initially, high NaCl tolerance seems to be determined by enhanced glycerol production, due to an increased expression of DhGPD1 and DhGPP2 (AL436338) in D. hansenii and GPD1 and GPP2 in S. cerevisiae; however, the ability to obtain high levels of intracellular glycerol seems to be more important. The two D. hansenii strains had higher levels of intracellular glycerol than the S. cerevisiae strain and were able to obtain high levels of intracellular glycerol, even at very high NaCl concentrations, indicating the presence of, for example, a type of closing channel, as previously described for other yeast species. Copyright © 2005 John Wiley & Sons, Ltd.
Cloning and characterization of a dextranase gene from Lipomyces starkeyi and its expression in Saccharomyces cerevisiae
Authors: Kang Hee‐Kyoung , Kim Seung Heuk , Park Ji‐Young , Jin Xing‐Ji , Oh Deok‐Kun , Soo Kang Seong , Kim Doman 
Published: November 2005

AbstractA dextranase‐encoding cDNA from L. starkeyi KSM22 was isolated and characterized. The 2052 bp cDNA fragment (lsd1) harbouring the dextranase gene exhibited one open reading frame (ORF) composed of 1824 bp flanked by a 41 bp 5′‐UTR and a 184 bp 3′‐UTR, including a 27 bp poly(A) tail. The lsd1 gene contains no introns. The open reading frame encodes a 608 amino acid polypeptide (LSD1) with a 67.6 kDa predicted molecular mass. There was a 77% deduced amino acid sequence identity between the LSD1 dextranase and the dextranase from Penicillium minioluteum. The primary structure of LSD1 dextranase exhibits distant similarity with the enzymes of the glycosyl hydrolase family 49 that comprises Penicillium dextranase. The optimum pH of LSD1 was 6.0 and the optimum temperature was 37 °C. LSD1 dextranase activity was substantially abolished by exposure to 1 mM Hg2+, Ag3+ and Mn2+. LSD1 exhibited high hydrolysing activity towards dextran (100%), soluble starch (22%) and mutan (8%). Copyright © 2005 John Wiley & Sons, Ltd.
Tension‐free vaginal tape for elderly women with stress urinary incontinence
Authors: Liapis A. , Bakas P. , Christopoulos P. , Giner M. , Creatsas G. 
Published: November 2005

AbstractObjective: To investigate the efficacy of the tension‐free vaginal tape (TVT) procedure for the management of stress urinary incontinence (SUI) in elderly women. Method: A total of 55 women aged between 65 and 86 years underwent a TVT procedure for urodynamic SUI. Of these, 15 (27%) had undergone previous surgery for treatment of SUI. Before the TVT procedure, a complete medical history was taken and a gynecologic examination performed. Results: Operating time ranged between 11 and 35 min (excluding the time of concomitant surgery, if any); hospitalization time ranged between 1 and 5 days; and no severe intraoperative or postoperative complications occurred. Cure occurred in 39 (76%) of 51 evaluable patients and its rate was positively associated with bladder neck mobility. Among patients in whom the angle of displacement on the Q‐tip test was less than 30°, 42% became continent whereas among those in whom it was 30° or higher, 90% became continent (P < .001). Among those in whom the angle was between 20° and 30°, 57% became continent, and among those in whom it was less than 10°, 80% remained incontinent. Conclusion: The TVT procedure in elderly women with SUI offers a satisfactory cure rate; however, in patients with significantly decreased bladder neck mobility (an angle < 20° on the Q‐tip test), the results are not encouraging.
Toronto Facial Grading System: Interobserver reliability
Authors: Kayhan Fatma Tulin , Zurakowski David , Rauch Steven D. 
Published: November 2005

The Toronto Facial Grading System (TFGS) is an observer scale for rating facial nerve dysfunction. The TFGS scores aspects of resting symmetry, symmetry of voluntary movement, and synkinesis for each division of the face (subscores) and then provides calculated total scores and an overall composite score of facial function. The developers of the scale have validated its sensitivity for identifying small changes in facial dysfunction and the independence of the different components measured. Herein we report our results in a study of interob‐server reliability using the TFGS. Twenty‐five patients from the Massachusetts Eye and Ear Infirmary Facial Nerve Center with varying degrees of facial paresis, paralysis, and synkinesis were videotaped, and the video recordings were scored by 5 independent observers using the TFGS. Intraclass correlation coefficients (κ) and 95% confidence intervals were calculated for subscores and for each total and composite score. Intraclass correlation coefficients ranged from 0.59 to 0.85, all considered substantial to near‐perfect agreement between observers. We believe the TFGS is superior to other scales by virtue of its sensitivity, comprehensiveness, ease of use, and interobserver reliability. The TFGS presently appears to be the best option in those situations in which accurate and precise documentation of facial function is required.
Remifentanil‐based anesthesia versus a propofol technique for otologic surgical procedures
Authors: Jellish W. Scott , Leonetti John P. , Avramov Avram , Fluder Elaine , Murdoch John 
Published: November 2005

Otologic procedures require a still surgical field and are associated with a 50% incidence of emetic symptoms. Propofol reduces nausea and vomiting but not intraoperative movement. This study compares a remifentanil/propofol anesthetic to a propofol/fentanyl combination to determine which provides the best perioperative conditions for otologic microsurgery. Eighty healthy patients were randomly assigned to receive one of the anesthetic combinations. Demographic data, hemodynamic variables, movement, and bispectral index monitoring values in addition to anesthetic emergence, nausea, vomiting, pain, and other recovery variables were compared between groups with appropriate statistical methods. Both groups were similar. Times to eye opening (7.7 ± 0.7 vs 12.4 ±1.2 minutes) and extubation (9.8 ± 0.9 vs 12.4 ±1.0 minutes) were shorter with remifentanil. This group also had lower hemodynamic variables and movement (23% vs 65%) under anesthesia. Postoperative pain was mild in both groups, but remifentanil patients had more than the propofol group. All other postoperative parameters were similar. Remifentanil‐based anesthesia produces better hemodynamic stability, less movement, and faster emergence after otologic surgery, with propofol's antiemetic effect, for the same cost.
Assessment of auditory cortex activation with functional magnetic resonance imaging
Authors: Ulualp Seckin O. , Biswal Bharat B. , Yetkin F. Zerrin , Kidder Thomas M. 
Published: November 2005

OBJECTIVEThe goal was to assess auditory cortex activation evoked by pure‐tone stimulus with functional MRI.METHODSFive healthy children, aged 7 to 10 years, were studied. Hearing evaluation was performed by pure‐tone audiometry in a sound‐treated room and in the MRI scanner with the scanner noise in the background. Subjects were asked to listen to pure tones (500, 1000, 2000, and 4000 Hz) at thresholds determined in the MRI scanner. Functional image processing was performed with a cross‐correlation technique with a correlation coefficient of 0.5 (P < 0.0001). Auditory cortex activation was assessed by observing activated pixels in functional images.RESULTSFunctional images of auditory cortex activation were obtained in 3 children. All children showed activation in Heschl's gyrus, middle temporal gyrus, superior temporal gyrus, and planum temporale. The number of activated pixels in auditory cortexes ranged from 4 to 33.CONCLUSIONSFunctional images of auditory cortex activation evoked by pure‐tone stimuli are obtained in healthy children with the functional MRI technique.
Electroneurographic facial muscle pattern in Bell's palsy
Authors: Engström Mats , Jonsson Lars , Grindlund Margareta , Stälberg Erik 
Published: November 2005

To study the electroneurographic facial muscle pattern in Bell's palsy over time, electroneurographic recordings in the frontalis, orbicularis oculi, nasalis, and mentalis muscle regions were performed early (mean, day 11) and 1 and 3 months after the onset of the condition in 30 consecutive patients. The correlation between facial muscle electroneurographic recordings over time was also calculated. An additional aim was to assess whether further prognostic information could be obtained by electroneurographic recordings in more than one facial region. The recovery pattern was similar in all 4 facial regions. Initially, the correlation between the facial recordings was weak (r = 0.20‐0.27), but it was improved at follow‐up examinations (r = 0.33‐0.65). Favorable outcome in 23 of 24 patients (96%) could have been predicted by the initial nasalis and/or mentalis recordings. The gap between patients with favorable outcome and patients with unfavorable outcome increased when the average electroneurography values were calculated from 1, 2, and 4 muscle recordings (4%, 8%, and 15%, respectively). Our results indicate that in Bell's palsy, electroneurographic examination of more than one facial muscle region may add prognostic information and that the degree of degeneration is initially different in the nerve branches.