bluuebunny/crossref_metadata_2025_split huggingface dataset:
Label‐Free Single Microparticles and Cell Aggregates Sorting in Continuous Cell‐Based Manufacturing
Authors: Gong Lingyan , He Linwei , Lu Nan , Petchakup Chayakorn , Li King Ho Holden , Tay Chor Yong , Hou Han Wei 
Published: March 2024

AbstractThere is a paradigm shift in biomanufacturing toward continuous bioprocessing but cell‐based manufacturing using adherent and suspension cultures, including microcarriers, hydrogel microparticles, and 3D cell aggregates, remains challenging due to the lack of efficient in‐line bioprocess monitoring and cell harvesting tools. Herein, a novel label‐free microfluidic platform for high throughput (≈50 particles/sec) impedance bioanalysis of biomass, cell viability, and stem cell differentiation at single particle resolution is reported. The device is integrated with a real‐time piezo‐actuated particle sorter based on user‐defined multi‐frequency impedance signatures. Biomass profiling of Cytodex‐3 microcarriers seeded with adipose‐derived mesenchymal stem cells (ADSCs) is first performed to sort well‐seeded or confluent microcarriers for downstream culture or harvesting, respectively. Next, impedance‐based isolation of microcarriers with osteogenic differentiated ADSCs is demonstrated, which is validated with a twofold increase of calcium content in sorted ADSCs. Impedance profiling of heterogenous ADSCs‐encapsulated hydrogel (alginate) microparticles and 3D ADSC aggregate mixtures is also performed to sort particles with high biomass and cell viability to improve cell quality. Overall, the scalable microfluidic platform technology enables in‐line sample processing from bioreactors directly and automated analysis of cell quality attributes to maximize cell yield and improve the control of cell quality in continuous cell‐based manufacturing.
Mixed‐Dimensional Membranes with Double 0D@2D Structures Enable Efficient and Sustainable Water Treatment
Authors: Zhang Zixin , Fu Huaqiang , Wang Zhe , Qian Wei , Zhao Xin , Si Yunfa , Guo Jiannan , Cao Shaowen , He Daping 
Published: March 2024

AbstractHigh‐efficiency and sustainable membrane purification technology is highly desirable but unattainable in the field of water treatment. 2D materials are emerging as very promising candidates for water treatment applications. However, membranes assembled by pure 2D materials lack reasonable structural and functional design, usually exhibiting relatively low water permeance, purification performance, and fouling resistance. Here, a mixed‐dimensional membrane is designed to solve the above‐mentioned problems by assembling two kinds of in situ grown 0D@2D functional building blocks of SiO2@GO and TiO2@MXene. Specifically, the obtained membrane exhibits high and maintainable water permeance of 2114 L m−2 h−1 bar−1 as well as high rejection rates toward organic dyes thanks to the plentiful firm water channels constructed by 0D@2D structures and the negative charges on both SiO2@GO and TiO2@MXene, respectively. Moreover, the 0D@2D TiO2@MXene serves as photocatalyst, rendering the composite membrane intriguing self‐cleaning function under UV irradiation, which is verified by the high recovery ratios of water permeance (97.5%) and dye interception rate (98.7%). Thus, this work offers a promising approach for the design of highly efficient and sustainable membrane for separation and purification purposes.
Clinical and Translational Opportunities of Nanocarriers Containing RNAi for the Management of Triple‐Negative Breast Cancer
Authors: Sharma Saurabh , Pradhan Rajesh , Lee David , Sharma Mahalaxmi , Nasab Shima Zahed , Hejmady Siddhanth , Chander Subhash , Mohaghegh Neda , Maity Surjendu , Khan Shahid , Khademhosseini Ali , Kirane Amanda 
Published: March 2024

AbstractTriple‐negative breast cancer develops from malignant cells within the interior lining of milk ducts or, less frequently, in the stromal tissues and accounts for ≈15% of all breast cancers. Breast cancer is highly prevalent with the overall number of cases expected to reach 27.78 million by 2040, according to the World Health Organization. MiRNAs serve a novel role in management of breast cancer through their ability to indirectly control the translation of coding mRNA and regulate the expression of specific genes, which allows it to play a significant role in cancer therapeutics. However, inherent characteristics of miRNA including rapid clearance via renal excretion, degradation by nucleases in plasma, and additional factors limit its clinical application. To reduce these limitations, miRNA can be administered in conjunction small molecules with nanocarriers, which then allow it to impart its benefits including inhibition of epithelial–mesenchymal transitions, and inhibiting tumor angiogenesis. This review highlights the current application of nanocarrier‐based delivery approach of miRNA/siRNA for triple negative breast cancer treatment.
Oncolytic Viruses in Cancer Immunotherapy
Authors: Li Xiao , Cheng Zhongping 
Published: March 2024

AbstractOncolytic viruses are novel and promising therapeutic regimens that promote antitumor efficacy through multimode mechanisms, including direct lysis of tumor cells, release of the tumor antigens and danger signals, induction of immunological cell death, activation of innate, and adaptive immune response. In addition, oncolytic viruses can be engineered to express virus‐centered and immune‐centered therapeutic transgenes, further transforming the “cold” tumor into “hot” and enhancing the oncolytic efficacy. Several oncolytic viruses are engineered and widely used as potential therapeutic agents for many cancers and the therapeutic strategies and effectiveness vary among different types of oncolytic viruses. Finally, oncolytic viruses‐based combination immunotherapy is practiced in preclinical and clinical studies, and indicates that combination of oncolytic viruses with conventional therapy or immunomodulatory agents exerts favorable antitumor efficacy.
Uniaxial‐Oriented Perovskite Films with Controllable Orientation
Authors: Li Dongni , Sun Xiangyu , Zhang Yao , Guan Zhen , Yue Yansong , Wang Qingya , Zhao Lu , Liu Fangze , Wei Jing , Li Hongbo 
Published: March 2024

AbstractPerovskite films with large crystal size, preferred orientation, and facile fabrication process, combining advantages of single‐crystal and polycrystalline films, have gained considerable attention recently. However, there is little research on the facet properties of perovskite films. Here, (111)‐ and (001)‐oriented perovskite films with bandgaps ranging from 1.53 to 1.77 eV, and systematically investigated their orientation‐dependent properties are achieved. The (111)‐oriented films show electron‐dominated traps and the (001)‐oriented films show hole‐dominated traps, which are related to their atomic arrangement at the surface. Compared with the (001)‐oriented films, the (111)‐oriented films exhibit lower work function and superior water/oxygen robustness. For the wide‐bandgap films, the lattice of the (001)‐oriented film provides an unobstructed passage for ion migration. Comparably, the (111)‐oriented films exhibit suppressed ion migration and excellent phase stability. The optimized unencapsulated solar cells based on both (001) and (111) orientations show a similar high efficiency of ≈23%. The (111)‐oriented solar cell exhibits excellent stability, maintaining 95% of its initial efficiency after 1500 h maximum power point (MPP) tracking test, and 97% initial efficiency after 3000 h aging in ambient conditions. This work paves the way for the rational design, controllable synthesis, and targeted optimization of uniaxial‐oriented perovskite films for various electronic applications.
The Trade‐Off between Transconductance and Speed for Vertical Organic Electrochemical Transistors
Authors: Skowrons Michael , Schander Andreas , Negron Alvaro Galeana Perez , Lüssem Björn 
Published: March 2024

AbstractThe high transconductance gm of organic electrochemical transistors (OECTs) has received widespread attention and made OECTs a valid candidate for wearable sensor systems. However, the large transconductance is often accompanied by large switching time constants, τ, making the transconductance an imperfect benchmark. For a fair assessment, the ratio of transconductance to switching time constant has to be considered instead of any single parameter in isolation. One approach put forward to optimize OECTs is a vertical design, in which the channel length can be scaled into the sub‐micrometer regime. Here, a new vertical device geometry is proposed, in which the active volume of the mixed conductor is confined to a small cavity between source and drain, yielding excellent performance and reproducibility. It is shown that this approach yields optimized ratios instead of maximized transconductance only. However, this scaling is effective only in a small range of device dimensions and requires careful optimization of the device to not be limited by parasitic effects such as excess volume of the mixed conductor or parasitic series resistances. Overall, the design considerations discussed here provide new guidelines to optimize OECTs, not only for high transconductance but for operation at high frequencies as well.
Systematic Review on Fabrication, Properties, and Applications of Advanced Materials in Wearable Photoplethysmography Sensors
Authors: Mathew Jinu , Zheng Dingchang , Xu Jianwei , Liu Haipeng 
Published: March 2024

AbstractPhotoplethysmography (PPG) technology enables the measurement of multiple physiological and psychological parameters with low‐cost wearable sensors and is reshaping modern healthcare. Advanced materials play a vital role in improving reliability and accuracy of PPG sensors. Recently, various advanced materials have been explored to optimize PPG sensor design, while some challenges exist toward large‐scale validation and mass production. This paper focuses on advanced materials applied in the photodetectors, light sources, and circuits of PPG sensors. The materials are categorized into four groups: inorganic, organic, nanomaterials, and hybrid materials. The properties and fabrication processes are summarized. Other technical details including the mode of operation, measurement sites, testing, and validation are discussed. The merits and limitations of the state of the art are highlighted to provide some suggestions for the future development of PPG sensors based on advanced materials.
Exploring and leveraging aggregation effects on reactive oxygen species generation in photodynamic therapy
Authors: Zhuang Zeyan , Li Jianqing , Shen Pingchuan , Zhao Zujin , Tang Ben Zhong 
Published: March 2024

AbstractAggregate‐level photodynamic therapy (PDT) has attracted significant interest and driven substantial advances in multifunction phototheranostic platforms. As exemplified by two typical instances of aggregation‐caused quenching of reactive oxygen species (ROS) and aggregation‐induced generation of ROS, the aggregation effect plays a significant role on the ROS generation of photosensitizers (PSs), which is worthy of in‐depth exploration and full utilization. However, in contrast to the well‐developed researches on the aggregation effect on luminescence, the studies concerning the aggregation effect on ROS generation are currently in a relatively nascent and disjointed stage, lacking guidance from a firmly established research paradigm. To advance this regard, this review aims at providing a consolidated overview of the fundamental principles and research status of aggregation effects on the ROS generation. Here, the research status can be organized into two main facets. One involves the comparison between isolated state and aggregated state, which is mainly conducted by two methods of changing solvent environments and adding adjuvants into a given solvent. The other underscores the distinctions between different aggregate states, consisting of three parts, namely comparison within the same or between different categories based on the classification of single‐component and multicomponent aggregates. In this endeavor, we will present our views on current research methodologies that explore how aggregation affects ROS generation and highlight the design strategies to leverage the aggregation effect to optimize PS regiments. We aspire this review to propel the advancement of phototheranostic platforms and accelerate the clinical implementation of precision medicine, and inspire more contributions to aggregate‐level photophysics and photochemistry, pushing the aggregate science and materials forward.
Combined balloon Eustachian tuboplasty/endoscopic sinus surgery for patients with chronic rhinosinusitis and Eustachian tube dysfunction
Authors: Hsieh Cheng‐Yu , Lin Wei‐Chieh , Lin Chung‐Ching , Chou Yi‐Fan 
Published: March 2024

AbstractBackgroundTo elucidate the role of balloon Eustachian tuboplasty (BET) in the management of chronic rhinosinusitis with obstructive Eustachian tube dysfunction (ETD), we evaluated the results of endoscopic sinus surgery (ESS) with and without BET in patients with chronic rhinosinusitis with obstructive ETD.MethodsThis randomized controlled trial conducted in a single‐institution tertiary care center setting included 50 patients diagnosed with primary chronic rhinosinusitis and obstructive ETD between July 2018 and June 2022. Twenty‐five patients were prospectively enrolled for combined ESS/BET. The control group (25 patients) underwent ESS alone. Outcome measurements of the Sinonasal Outcome Test 22, modified Lund–Kennedy score, Eustachian Tube Dysfunction Questionnaire‐7 (ETDQ‐7), and serial Eustachian tube function test results were analyzed 3 months postoperatively.ResultsThe improvement (12.60 ± 6.50) in the ETDQ‐7 score in the BET group was significantly higher than that in the control group (6.60 ± 5.58). The ratio of improvement in the ETDQ‐7 score was also significantly higher in the BET than in the control group (92% vs. 68%, p = 0.034). Logistic regression analysis showed that performing BET (odds ratio [OR]: 5.41, 95% confidence interval [CI]: 1.02–28.79, p = 0.048) and a low post‐modified Lund–Kennedy score (OR: 0.15, 95% CI: 0.04–0.54, p = 0.004) were significantly associated with ETDQ‐7 score improvement.ConclusionCombined BET/ESS could decrease otologic symptoms and improve Eustachian tube function. BET may be an appropriate adjunctive procedure for treating chronic rhinosinusitis with obstructive ETD.
The Inflation Reduction Act: Implications for Medicare spending and access to biologic therapies for chronic rhinosinusitis with nasal polyposis and asthma
Authors: Rathi Vinay K. , Soler Zachary M. , Schlosser Rodney J. , Workman Alan D. , Chapurin Nikita , Rowan Nicholas R. , Dusetzina Stacie B. 
Published: March 2024

Key points
In 2021, Medicare spending on biologics was $926 million in Part B (FFS) and $1.3 billion in Part D (FFS/MA).
Between 2017 and 2021, annual Medicare spending on biologics increased by approximately 200%.
Between 2023 and 2025, Medicare Part D OOP costs for biologics will decrease by an estimated 50%–60%.
Transesterification Induced Multifunctional Additives Enable High‐Performance Lithium Metal Batteries
Authors: Gao Yuanhang , Wu Gang , Fang Wenqiang , Qin Zuosu , Zhang Tao , Yan Jiaxing , Zhong Yunpeng , Zhang Ning , Chen Gen 
Published: March 2024

AbstractThe electrolyte chemistry is crucially important for promoting the practical application of lithium metal batteries (LMBs). Here, we demonstrate for the first time that 1,3‐dimethylimidazolium dimethyl phosphate (DIDP) and trimethylsilyl trifluoroacetate (TMSF) can undergo in situ transesterification in carbonate electrolyte to generate dimethyl trimethylsilyl phosphate (DTMSP) and 1,3‐dimethylimidazolium trifluoroacetate (DITFA) as multifunctional additives for LMBs. H2O and HF can be removed by the Si−O group in DTMSP to improve the moisture resistance of electrolyte and the stability of cathode. Furthermore, the dissolution of lithium nitrate (LiNO3) in carbonate electrolyte can be promoted by the trifluoroacetate anion (TFA−) in DITFA, thereby optimizing the solvation structure and transport kinetics of Li+. More importantly, both DTMSP and DITFA tend to preferential redox decomposition due to the low lowest unoccupied molecular orbital (LUMO) and high highest occupied molecular orbital (HOMO). Consequently, a thin and robust layer rich in P/N/Si on the cathode and an inorganic‐rich layer (e.g. Li3N/Li3P) on the anode can be constructed and superior electrochemical performances are achieved. This artificial transesterification strategy to introduce favorable additives paves an efficient and ingenious route to high‐performance electrolyte for LMBs.
A NIR‐II Photoacoustic Probe for High Spatial Quantitative Imaging of Tumor Nitric Oxide in Vivo
Authors: Jiang Zhiyong , Zhang Changli , Sun Qian , Wang Xiaoqing , Chen Yuncong , He Weijiang , Guo Zijian , Liu Zhipeng 
Published: March 2024

AbstractNitric oxide (NO) exhibits both pro‐ and anti‐tumor effects. Therefore, real‐time in vivo imaging and quantification of tumor NO dynamics are essential for understanding the conflicting roles of NO played in pathophysiology. The current molecular probes, however, cannot provide high‐resolution imaging in deep tissues, making them unsuitable for these purposes. Herein, we designed a photoacoustic probe with an absorption maximum beyond 1000 nm for high spatial quantitative imaging of in vivo tumor NO dynamics. The probe exhibits remarkable sensitivity, selective ratiometric response behavior, and good tumor‐targeting abilities, facilitating ratiometric imaging of tumor NO throughout tumor progression in a micron‐resolution level. Using the probe as the imaging agent, we successfully quantified NO dynamics in tumor, liver and kidney. We have pinpointed an essential concentration threshold of around 80 nmol/cm3 for NO, which plays a crucial role in the “double‐edged‐sword” function of NO in tumors. Furthermore, we revealed a reciprocal relationship between the NO concentration in tumors and that in the liver, providing initial insights into the possible NO‐mediated communication between tumor and the liver. We believe that the probe will help resolve conflicting aspects of NO biology and guide the design of imaging agents for tumor diagnosis and anti‐cancer drug screening.
Interface‐Compatible Gel‐Polymer Electrolyte Enabled by NaF‐Solubility‐Regulation toward All‐Climate Solid‐State Sodium Batteries
Authors: Guo Xiaoniu , Xie Zhengkun , Wang Ruixue , Luo Jun , Chen Jiacheng , Guo Shuai , Tang Guochuan , Shi Yu , Chen Weihua 
Published: March 2024

AbstractGel‐polymer electrolyte (GPE) is a pragmatic choice for high‐safety sodium batteries but still plagued by interfacial compatibility with both cathode and anode simultaneously. Here, salt‐in‐polymer fibers with NaF salt inlaid in polylactide (PLA) fiber network was fabricated via electrospinning and subsequent in situ forming gel‐polymer electrolyte in liquid electrolytes. The obtained PLA‐NaF GPE achieves a high ion conductivity (2.50×10−3 S cm−1) and large Na+ transference number (0.75) at ambient temperature. Notably, the dissolution of NaF salt occupies solvents leading to concentrated‐electrolyte environment, which facilitates aggregates with increased anionic coordination (anion/Na+ >1). Aggregates with higher HOMO realize the preferential oxidation on the cathode so that inorganic‐rich and stable CEI covers cathode’ surface, preventing particles’ breakage and showing good compatibility with different cathodes (Na3V2(PO4)3, Na2+2xFe2‐x(SO4)3, Na0.72Ni0.32Mn0.68O2, NaTi2(PO4)3). While, passivated Na anode induced by the lower LUMO of aggregates, and the lower surface tension between Na anode and PLA‐NaF GPE interface, leading to the dendrites‐free Na anode. As a result, the assembled Na || Na3V2(PO4)3 cells display excellent electrochemical performance at all‐climate conditions.
Photoactivated Circularly Polarized Luminescent Organic Radicals in Doped Amorphous Polymer
Authors: She Pengfei , Qin Yanyan , Zhou Yuxiang , Zheng Xiaokang , Li Feiyang , Liu Shujuan , Ma Yun , Zhao Qiang , Wong Wai‐Yeung 
Published: March 2024

AbstractLuminescent organic radicals, especially those with photoactivated circularly polarized luminescence (CPL) features, hold great significance for cutting‐edge optoelectronic applications, but their development still remains a challenge. In this study, we propose a novel strategy to achieve photoactivated CPL radicals by bonding two phosphine centers within an axial chiral system, yielding a compound of R/S‐5,5‐bis(diphenylphosphino)‐4,4′‐bibenzo[d][1,3]dioxole (R/S‐BDP). The photoactivated R/S‐BDP molecules in polymer matrix display a robust quantum yield of 19.8 % and a dissymmetry factor (glum) of 1.2×10−4, marking this work as the first example of photoactivated CPL radicals. Furthermore, the glum is improved to 1.0×10−2 by using a liquid crystal as host. Experimental and theoretical analyses reveal that R/S‐BDP molecules, endowed with double phosphine cores in axial chirality, offer a direct way for intramolecular electron transfer upon photoirradiation. This leads to the generation of radical ionic pairs, which subsequently trigger the donor‐acceptor arrangement through intermolecular electron transfer, thereby resulting in stable radical emission. The extended photoactivated BDP‐F exhibits a remarkably high quantum efficiency of 57.8%. Ultimately, the distinctive photo‐responsive CPL radical luminescence has been successfully used for information displays and anti‐counterfeiting.
Microencapsulation of pyriproxyfen using the particles from gas‐saturated solutions process as a controlled‐release system
Authors: Sharmin Tanjina , Tashiro Hiroyuki , Sakai Hiroki , Shibata Kouichiro , Ema Konoka , Irie Keiichi , Satho Tomomitsu , Mishima Kenji 
Published: March 2024

AbstractThe purpose of this investigation was to determine whether pyriproxyfen (PPF), a synthetic juvenile hormone analog (JHA), could be encapsulated in supercritical carbon dioxide (scCO2) using the process particles from the gas‐saturated solutions (PGSS) for a controlled‐release system. The PGSS process represents a promising two‐step production system especially suited for the encapsulation and controlled‐release system (CRS). In contrast to traditional encapsulation methods that often involve the use of harsh organic solvents or high temperatures, the PGSS process offers a gentler approach employing scCO2 as an alternative. The solubility of scCO2 in polymer (poly‐ϵ‐caprolactone [PCL]) allowed for the formation of PPF microparticles, and the particle size distribution could be controlled by adjustment of operating pressure and temperature. The obtained particles had a mean particle size of 73.6 ± 2 μm and encapsulation efficiency of 78.8 ± 9% at 60°C and 10 MPa. Furthermore, the in vitro dissolution profiles of PPF–PCL particles showed a low‐level release pattern (42.5 ± 5 ppb/d) in water, followed by zero‐order kinetics indicating a high‐performance CRS. Finally, the in vivo bioassay using microparticles treated water exhibited 95%–100% emergence inhibition of mosquitoes, suggesting the effectiveness of PPF–PCL particles as a mosquito control agent.