bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pengembangan Motorik Halus Anak Usia Dini melalui Permainan Pasir Kinetik di Kelompok B Pratama Widya Pasraman Gurukula
Authors: Suryawan I Gde , Kasriani Ni Wayan 
Published: February 2024

Early childhood is experiencing a process of rapid growth and development. In this case, stimulation is needed to develop aspects of child development. One of the child's development that needs to be developed is fine motor skills. Early childhood education is a means to develop children's fine motor skills through a variety of fun games. Kinetic sand play activities are activities that can develop children's fine motor skills. Kinetic sand is a learning medium in the form of sand that is flexible, easy to shape and liked by children. The purpose of this study was to determine motor development activities through playing kinetic sand in Group B Pratama Widya Pasraman Gurukula Bangli. This study uses qualitative methods to convey research results descriptively. Data collection techniques in this study are observation, interviews and documentation. The results of this study were that early childhood fine motor development activities in Group B Pratama Widya Pasraman Gurukula Bangli through kinetic sand games had a significant impact on children's fine motor development. By squeezing, holding, pressing and shaping kinetic sand, it can stimulate the muscles of the child's arms so that fine motor skills can develop properly.
Intervenciones para prevenir el sobrepeso y la obesidad en adolescentes
Authors: Medina Llatas Ana Paola Yomar , Vitón Vásquez Sharon , Valderrama Rios Olga Giovanna , Salazar Rojas Judit , Riojas-Yance Zora de los Ángeles , Hiyo-Huapaya Eva Patricia 
Published: February 2024

Objetivo: Analizar la evidencia científica sobre las estrategias de intervención para prevenir el sobrepeso y la obesidad en adolescentes. Material y métodos: Revisión sistemática de artículos publicados entre 2017 y 2022. Se revisaron artículos en las bases de datos Medline-PubMed, Scielo y Dialnet. Después de aplicar los criterios de inclusión y exclusión se seleccionaron 10 artículos. Se utilizó el diagrama de flujo Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA). Resultados: Se encontraron 30 manuscritos en Scielo, 70 manuscritos en Pubmed y 5 en Dialnet, que sumaron un total de 105 registros, de los cuales se seleccionaron 70 manuscritos a través de un análisis de los títulos y resúmenes. Asimismo, de ellos apenas 12 registros son textos completos, de los cuales 10 manuscritos fueron incluidos para el estudio: 1 en Scielo y 9 en PubMed. Conclusiones: En los 10 artículos analizados se evidenciaron diversos enfoques de intervención en aspectos nutricionales y actividad física. Los estudios abordaron estrategias de prevención de sobrepeso y obesidad en los adolescentes, incorporando a la familia; además, presentaron cambios en la reducción del índice de masa corporal (IMC) en periodos más largos.
Estrés y desempeño laboral en el personal de enfermería de la Clínica San Judas Tadeo, Lima - 2023
Authors: Gamarra Causto Idalia , Arevalo Marcos Rodolfo Amado 
Published: February 2024

Objetivo: Determinar la relación entre el estrés y el desempeño laboral en el personal de enfermería de la Clínica San Judas Tadeo, Lima - 2023. Materiales y métodos: El estudio se desarrolló a través del método hipotético deductivo, de tipo aplicado, de enfoque cuantitativo, de nivel correlacional, de diseño no experimental y de corte transversal; en una muestra de 80 participantes. La técnica utilizada fue la encuesta; los instrumentos de recolección de datos fueron dos, el cuestionario denominado Questionaries Nursing Stress Scale (N.S.S) y la escala de desempeño laboral, propuesta por el MINSA. Resultados: Los resultados encontrados evidencian que el 52,5% de los participantes presentaron regular nivel de estrés, el 31,3% estrés bajo y el 16,3% estrés alto. Asimismo, el 51.2% evidenciaron regular desempeño laboral, el 46,3% alto desempeño y el 2,5% deficiente desempeño laboral. Por otra parte, se encontró relación en las dimensiones física, psicológica y social del estrés, con el desempeño laboral. Conclusiones: Se concluye determinado que existe relación entre el estrés y el desempeño laboral en los profesionales de enfermería, donde el valor de p fue igual a 0,000 < 0,05; y el resultado de la correlación de Spearman fue positiva moderada (Rho = 4,71).
Use of patellofemoral digital twins for patellar tracking and treatment prediction: comparison of 3D models and contact detection algorithms
Authors: Michaud Florian , Luaces Alberto , Mouzo Francisco , Cuadrado Javier 
Published: February 2024

Introduction: Poor patellar tracking can result in painful contact pressures, patella subluxation, or dislocation. The use of musculoskeletal models and simulations in orthopedic surgeries allows for objective predictions of post-treatment function, empowering clinicians to explore diverse treatment options for patients. Although a promising approach for managing knee surgeries, the high computational cost of the Finite Element Method hampers its clinical usability. In anticipation of minimal elastic deformations in the involved bodies, the exploration of the Multibody Dynamics approach emerged as a viable solution, providing a computationally efficient methodology to address clinical concerns related to the knee joint.Methods: This work, with a focus on high-performance computing, achieved the simulation of the patellofemoral joint through rigid-body multibody dynamics formulations. A comparison was made between two collision detection algorithms employed in the simulation of contact between the patellar and femoral implants: a generic mesh-to-mesh collision detection algorithm, which identifies potential collisions between bodies by checking for proximity or overlap between their discretized mesh surface elements, and an analytical contact algorithm, which uses a mathematical model to provide closed-form solutions for specific contact problems, but cannot handle arbitrary geometries. In addition, different digital twins (3D model geometries) of the femoral implant were compared.Results: Computational efficiency was considered, and histories of position, orientation, and contact force of the patella during the motion were compared with experimental measurements obtained from a sensorized 3D-printed test bench under pathological and treatment scenarios. The best results were achieved through a purely analytical contact detection algorithm, allowing for clinical usability and optimization of clinical outcomes.
In-silico techniques to inform and improve the personalized prescription of shoe insoles
Authors: Killen Bryce A. , Van Rossom Sam , Burg Fien , Vander Sloten Jos , Jonkers Ilse 
Published: February 2024

Background: Corrective shoe insoles are prescribed for a range of foot deformities and are typically designed based on a subjective assessment limiting personalization and potentially leading to sub optimal treatment outcomes. The incorporation of in silico techniques in the design and customization of insoles may improve personalized correction and hence insole efficiency.Methods: We developed an in silico workflow for insole design and customization using a combination of measured motion capture, inverse musculoskeletal modelling as well as forward simulation approaches to predict the kinematic response to specific insole designs. The developed workflow was tested on twenty-seven participants containing a combination of healthy participants (7) and patients with flatfoot deformity (20).Results: Average error between measured and simulated kinematics were 4.7 ± 3.1, 4.5 ± 3.1, 2.3 ± 2.3, and 2.3 ± 2.7° for the chopart obliquity, chopart anterior-posterior axis, tarsometatarsal first ray, and tarsometatarsal fifth ray joints respectively.Discussion: The developed workflow offers distinct advantages to previous modeling workflows such as speed of use, use of more accessible data, use of only open-source software, and is highly automated. It provides a solid basis for future work on improving predictive accuracy by adapting the currently implemented insole model and incorporating additional data such as plantar pressure.
Placebo-resistant gut bacteria: Akkermansia muciniphila spp. and Familial Mediterranean fever disease
Authors: Pepoyan Elya , Marotta Francesco , Manvelyan Anahit , Galstyan Artak , Stepanyan Lena , Grigoryan Hasmik , Grigoryan Liana , Mikayelyan Mikayel , Balayan Marine , Harutyunyan Natalya , Mirzabekyan Susanna , Tsaturyan Vardan , Torok Tamas , Pepoyan Astghik 
Published: February 2024

IntroductionDespite numerous investigations into the impact of drugs/probiotics on the gut microbiota composition in Familial Mediterranean Fever (FMF) patients, the question as to whether there exists a significant bacterial diversity(ies) independent of the placebo effect that can be reliably considered in clinical and nutritional trials remains unresolved.MethodsThis study represents the in augural analysis of the placebo’s influence on the gut microbiota of both healthy individuals and FMF afflicted men, utilizing previously collected data from PhyloChip™ DNA microarray experiments. A total of 15 healthy and 15 FMF male volunteers, aged 18 to 50, participated in this partially randomized placebo trial, which is accessible through the GEO Series accession number GSE111835.Results and DiscussionKey findings from current investigations include i. the anticipated divergence in gut bacteria resistance to placebo between healthy and FMF individuals, ii. the minor impact of placebo on gut bacterial diversities in healthy individuals, with Enterobacteriaceae diversities identified as placebo-resistant among “healthy” gut bacteria, and iii. the comprehensive influence of placebo on all bacterial phyla in the gut microbiome of FMF patients, extending to nearly all bacterial genera, except for the resilience of gut Akkermansia muciniphila spp. to placebo in FMF patients. This study underscores the susceptibility of Faecalibacterium, Blautia, and Clostridium genera to placebo. Consequently, this investigation holds significance for the proper design of placebo-controlled trials and establishes a foundation for further exploration of the gut-brain axis. Furthermore, it contributes valuable insights to discussions regarding proposals for probiotic therapies, particularly focusing on Faecalibacterium spp., Blautia spp., and Clostridium spp.
Efficacy of physical exercise on the physical ability, cardiac function and cardiopulmonary fitness of patients with atrial fibrillation: a systematic review and meta-analysis
Authors: Yang Chenyan , Wu Qian , Lv Qianyu , Hou Xinzheng , Ye Xuejiao , Yang Yingtian , Li Lanlan , Zuo Wenxi , Wang Shihan 
Published: February 2024

ObjectiveIt is advised that patients engage in physical activity to enhance their quality of life and achieve better results. The purpose of the current study was to measure the efficacy of exercise on the physical ability, cardiac function and cardiopulmonary fitness of patients with AF.MethodA comprehensive systematic literature search was performed in PubMed, Embase, and Web of Science from 1991 to 2023 for RCTs comparing physical exercise combined with AF routine treatments to routine treatments alone. The meta-analysis was conducted following PRISMA guidelines. Our main outcomes were physical ability (measured by the 6-min walk test, 6MWT), cardiac function (measured by left ventricular ejection fraction, LVEF) and cardiopulmonary fitness (measured by peak oxygen uptake and resting heart rate). Quality assessments were conducted using the Cochrane Collaboration tool.ResultsThirteen trials involving 672 patients met the criteria for analysis. The results showed that physical exercise increased physical ability by improving the 6MWT (m) performance (MD = 96.99, 95% CI: 25.55–168.43; Z = 2.66; p = 0.008); and enhanced peak VO2 (ml/kg per min) (MD = 4.85, 95% CI: 1.55–8.14; Z = 2.89; p = 0.004) while reducing resting heart rate (beats per minute, bpm) (MD = −6.14, 95% CI: −11.30 to −0.98; Z = 2.33; p = 0.02). However, the results showed that regular exercise could improve LVEF (%) inpatients clinically, which had no statistic difference between experimental and control group (MD = 1.49, 95% CI: −0.25–3.24; Z = 1.68; p = 0.09).ConclusionOur meta-analysis shows that physical exercise is an effective intervention to improve the exercise ability and cardiopulmonary fitness for AF patients. Meanwhile, we also do not exclude the positive effect of exercise on the improvement of cardiac function (LVEF) in patients with AF. To this end, doctors should consider the positive impact of exercise on patients and give advice on exercise limits in practical clinical practice.
Ovaries of estrogen receptor 1-deficient mice show iron overload and signs of aging
Authors: Schröder Sarah K. , Krizanac Marinela , Kim Philipp , Kessel Jan C. , Weiskirchen Ralf 
Published: February 2024

IntroductionEstrogens are crucial regulators of ovarian function, mediating their signaling through binding to estrogen receptors. The disruption of the estrogen receptor 1 (Esr1) provokes infertility associated with a hemorrhagic, cystic phenotype similar to that seen in diseased or aged ovaries. Our previous study indicated the possibility of altered iron metabolism in Esr1-deficient ovaries showing massive expression of lipocalin 2, a regulator of iron homeostasis.MethodsTherefore, we examined the consequences of depleting Esr1 in mouse ovaries, focusing on iron metabolism. For that reason, we compared ovaries of adult Esr1-deficient animals and age-matched wild type littermates. Results and discussionWe found increased iron accumulation in Esr1-deficient animals by using laser ablation inductively coupled plasma mass spectrometry. Western blot analysis and RT-qPCR confirmed that iron overload alters iron transport, storage and regulation. In addition, trivalent iron deposits in form of hemosiderin were detected in Esr1-deficient ovarian stroma. The depletion of Esr1 was further associated with an aberrant immune cell landscape characterized by the appearance of macrophage-derived multinucleated giant cells (MNGCs) and increased quantities of macrophages, particularly M2-like macrophages. Similar to reproductively aged animals, MNGCs in Esr1-deficient ovaries were characterized by iron accumulation and strong autofluorescence. Finally, deletion of Esr1 led to a significant increase in ovarian mast cells, involved in iron-mediated foam cell formation. Given that these findings are characteristics of ovarian aging, our data suggest that Esr1 deficiency triggers mechanisms similar to those associated with aging.
Discussion on the mechanism of Danggui Sini decoction in treating diabetic foot based on network pharmacology and molecular docking and verification of the curative effect by meta-analysis
Authors: Ni Xiyu , Bao Huhe , Guo Jiaxing , Li Deer , Wang Lihang , Zhang Wanyin , Sun Guanwen 
Published: February 2024

ObjectiveThe main active components and mechanism of Danggui Sini decoction (DSD) in treating diabetic foot (DF) were studied and verified by network pharmacology and molecular docking. Evidence-based medicine was used to prove its efficacy.MethodsThe TCMSP systematic pharmacology platform screened out DSD’s practical components and targets—screening disease targets in GeneCards database, using Cytoscape 3.7.2 to draw DSD–active ingredient–target network diagram, and drawing the protein interaction network diagram through STRING database. The Metascape platform was used to analyze the GO function enrichment and KEGG signal pathway. The molecular docking experiment was carried out by using Auto Dock vina 4.2. The related literature on DSD in treating DF in China Zhiwang, Wanfang, Weipu, and China Biomedical Literature Database was searched. The literature was screened, data was extracted, and quality was evaluated according to the inclusion and exclusion criteria. Then, a meta-analysis was performed using RevMan 5.3 software.ResultsA total of 256 targets of all effective components of DSD were obtained. Among 1,272 disease targets, there are 113 common targets. The GO analysis received 6,179 entries, and the KEGG pathway enrichment analysis found 251 related pathways. The molecular docking results of the main targets of diabetic foot and the active substances of DSD all showed a high docking activity. The meta-analysis included six literature, all of which were randomized controlled experiments. The quality grade of the literature was C, and the results showed that the total effective rate of clinical efficacy in the experimental group was significantly higher than that in the control group.ConclusionsDSD may treat DF by participating in biological processes such as cell proliferation regulation, inflammatory reaction, oxidative stress reaction, and promotion of angiogenesis. DSD treats DF through AKT1, TP53, IL6, TNF, VEGFA, and other targets. DSD plays a role in treating DF mainly through the AGE-RAGE signaling pathway and PI3K-AKT signaling pathway. The molecular docking results of AKT1, TP53, IL-6, TNF, and VEGFA with the active substances of DSD show that they all have a high docking activity; among them, VEGFA has a higher docking activity. Compared with conventional treatment, DSD has a high effective rate, short wound healing time, large wound healing area, and high ABI index.
Extracellular vesicles released by microglia and macrophages carry endocannabinoids which foster oligodendrocyte differentiation
Authors: Lombardi Marta , Scaroni Federica , Gabrielli Martina , Raffaele Stefano , Bonfanti Elisabetta , Filipello Fabia , Giussani Paola , Picciolini Silvia , de Rosbo Nicole Kerlero , Uccelli Antonio , Golia Maria Teresa , D’Arrigo Giulia , Rubino Tiziana , Hooshmand Kourosh , Legido-Quigley Cristina , Fenoglio Chiara , Gualerzi Alice , Fumagalli Marta , Verderio Claudia 
Published: February 2024

IntroductionMicroglia and macrophages can influence the evolution of myelin lesions through the production of extracellular vesicles (EVs). While microglial EVs promote in vitro differentiation of oligodendrocyte precursor cells (OPCs), whether EVs derived from macrophages aid or limit OPC maturation is unknown.MethodsImmunofluorescence analysis for the myelin protein MBP was employed to evaluate the impact of EVs from primary rat macrophages on cultured OPC differentiation. Raman spectroscopy and liquid chromatography-mass spectrometry was used to define the promyelinating lipid components of myelin EVs obtained in vitro and isolated from human plasma.Results and discussionHere we show that macrophage-derived EVs do not promote OPC differentiation, and those released from macrophages polarized towards an inflammatory state inhibit OPC maturation. However, their lipid cargo promotes OPC maturation in a similar manner to microglial EVs. We identify the promyelinating endocannabinoids anandamide and 2-arachidonoylglycerol in EVs released by both macrophages and microglia in vitro and circulating in human plasma. Analysis of OPC differentiation in the presence of the endocannabinoid receptor antagonists SR141716A and AM630 reveals a key role of vesicular endocannabinoids in OPC maturation. From this study, EV-associated endocannabinoids emerge as important mediators in microglia/macrophage-oligodendrocyte crosstalk, which may be exploited to enhance myelin repair.
Identification of marker genes for spinal cord injury
Authors: Luan Zhiwei , Zhang Jiayu , Wang Yansong 
Published: February 2024

IntroductionSpinal cord injury (SCI) is a profoundly disabling and devastating neurological condition, significantly impacting patients’ quality of life. It imposes unbearable psychological and economic pressure on both patients and their families, as well as placing a heavy burden on society.MethodsIn this study, we integrated datasets GSE5296 and GSE47681 as training groups, analyzed gene variances between sham group and SCI group mice, and conducted Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis based on the differentially expressed genes. Subsequently, we performed Weighted Gene Correlation Network Analysis (WGCNA) and Lasso regression analyses.ResultsWe identified four characteristic disease genes: Icam1, Ch25h, Plaur and Tm4sf1. We examined the relationship between SCI and immune cells, and validated the expression of the identified disease-related genes in SCI rats using PCR and immunohistochemistry experiments.DiscussionIn conclusion, we have identified and verified four genes related to SCI: Icam1, Ch25h, Plaur and Tm4sf1, which could offer insights for SCI treatment.
Mentha rotundifolia (L.) Huds. and Salvia officinalis L. hydrosols mitigate aging related comorbidities in rats
Authors: Boualam Khadija , Ibork Hind , Lahboub Zakaria , Sobeh Mansour , Taghzouti Khalid 
Published: February 2024

IntroductionAging is often linked to oxidative stress, where the body experiences increased damage from free radicals. Plants are rich sources of antioxidants, playing a role in slowing down aging and supporting the proper functioning and longevity of cells. Our study focuses on exploring the impact of Mentha rotundifolia (MR) and Salvia officinalis (SO) hydrosols on aging-related comorbidities.MethodsThe chemical composition of MR and SO hydrosols was analyzed by gas chromatography coupled to mass spectrometry. 2,2-Diphenyl 1-picrylhydrazyl and 2,20-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid radicals scavenging assays were used to assess their in vitro antioxidant activity, and heat induced albumin denaturation test was used to evaluate their anti-inflammatory activity. Subsequently, we administered 5% of each plant hydrosol in the drinking water of 18-month-old rats for six months. We then conducted behavioral tests, including open field, dark/light box, rotarod, and Y-maze assessments, and measured biochemical parameters in plasma, liver and brain tissues.Results and discussionAt two years old, animals treated with MR and SO hydrosols displayed fewer physical and behavioral impairments, along with well-preserved redox homeostasis in comparison with animals in the control group. These results highlighted the significance of MR and SO hydrosols in addressing various aspects of age-related comorbidities. The study suggests that these plant-derived hydrosols may have potential applications in promoting healthy aging and mitigating associated health challenges.