bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic adoptive transfer of immunity and low‐dose irradiation eradicate metastases of 13762a rat mammary adenocarcinoma
Authors: Kreider John W. , Bartlett Gerald L. , Christensen Neil D. , Welsh Patricia 
Published: February 2007

AbstractRats cured of poorly immunogenic 13762A tumor by a combination of surgery and cyclophosphamide (CY) treatments produced peritoneal exudate cells (PEC) that prevented tumor growth when transferred to naive recipients, but they were ineffective against established tumor. A highly immunogenic 13762A clone (18A) induced PEC lymphocytes that completely reversed the growth of established primary tumor and of lymph‐node metastases. 18A‐immune PEC alone strongly inhibited tumors of 7 days' duration, but only moderately suppressed 14‐day tumors, and had no effect on 21‐day tumors. Irradiation (450 R) of rats prior to tumor transplantation improved the effectiveness of the PEC given at 7 days, but the benefit had gone by 14 days. Long‐term T‐cell depletion prior to tumor challenge allowed PEC inhibition of 7‐ and 14‐day tumors, but not 21‐day tumors. The most potent strategy was the administration of 450 R followed immediately by immune PEC. When rats with 21‐day tumors were so treated, the metastases grew temporarily to a maximum diameter of 2‐5 cm and then completely regressed. We concluded that a combination of immune T cells and 450 R can cure established, massive metastases, probably through a combination of an increase in the numbers of T‐cell effectors and elimination of suppressor cells.
Production of PDGF‐like growth factor by breast cancer cell lines
Authors: Rozengurt Enrique , Sinnett‐Smith James , Taylor‐Papadimitriou Joyce 
Published: February 2007

AbstractThe breast cancer cell line T47D produces factors which show mitogenic activity for 3T3 cells. Here we describe the partial purification of one of these factors and show that it has properties similar to plateletderived growth factor (PDGF). The T47D factor is a heat‐stable hydrophobic protein with a molecular weight of around 30,000, which inhibits the binding of 125I‐EGF in a temperature‐dependent manner. This 30 kd protein does not act synergistically with PDGF or fibroblast‐derived growth factor (FDGF; also PDGF‐like) in stimulating DNA synthesis; moreover, like these two factors, its mitogenic activity can be inhibited by an antiserum raised against human PDGF. A PDGF‐like growth factor was also found in the serum free medium of the breast cancer cell line MDA‐MB157. Since PDGF acts on mesenchymal cells, the production of PDGF like growth factors by breast cancer cells suggests a basis for the intense stromal reaction seen in human breast cancers.
Selection and characterization of monoclonal antibodies to the idiotype‐like structure of an interleukin‐2‐producing human leukemia t‐cell line
Authors: Moretta Alessandro , Pantaleo Giuseppe , Lopez‐Botet Miguel , Moretta Lorenzo 
Published: February 2007

AbstractA variant of the Jurkat leukemia cell line termed JA3 (surface phenotype: T11 +, T3 +, T4‐, T8‐, T6‐, T1 +, 3A1 +, HLA‐DR‐, Tac‐, 4FZ+) has been used for mouse immunization and production of monoclonal antibodies (MAbs) to molecules carrying clonotypic determinants that are thought to serve as receptor for antigen on human T cells. JA3 had been selected because of its ability to release large amounts of IL‐2 following stimulation with phytohemagglutinin (PHA) or anti‐T3 antibody in the presence of phorbolmyristate acetate (PMA). This functional property has been exploited for screening of MAbs potentially directed to idiotype‐like structures of JA3 cells. Thus supernatants of hybridomas (obtained by fusing mouse splenocytes with P3‐UI myeloma cells) were analyzed for their ability to induce JA3 cells to release IL‐Z in the presence of PMA. Four stimulatory antibodies reacted with JA3 but not with polyclonal T‐cell populations, T‐cell clones, or T and B tumor cell lines as assessed by indirect immunofluorescence and fluorescence‐activated cell sorter (FACS) analysis. All 4 antibodies immunoprecipitated the same disulphide‐linked heterodimeric molecules having a molecular mass (M′) of approximately 85,000 under non‐reducing conditions that was resolved in Z major peptides of 40,000 and 45,000 under reducing conditions. These data indicate that these antibodies (termed anti‐JTi) were directed to the clonotypic‐restricted structures of the JA3 T‐cell ‐ receptor molecules. Unlike the anticlonotypic antibodies described so far, anti‐JTi MAbs were capable of triggering IL‐Z production even in unbound, soluble form, in the absence of adherent cells or PMA. Competitive inhibition experiments, in which 35S‐la‐belled anti‐JTi MAbs have been used, provided evidence that they may be directed against different epitopes on the same clonotypic structure.
The establishment and characterization of a new balb/c angiosarcoma tumor system
Authors: Zvibel Isabel , Raz Avraham 
Published: February 2007

AbstractA BALB‐3T3/A31 untransformed cloned cell line and 3 selected variants derived from this parental cell line, expressing 3 increasingly malignant phenotypes, have been established and characterized in vivo and in culture. This new tumor series, identified as angiosarcoma, consists of an anchorage‐independent nontumorigenic cell clone and 2 sublines exhibiting tumorigenic and metastatic properties. Morphological examination revealed that the 3 transformed cell variants differed from the normal parental cells and were not contact‐inhibited. Karyotype and rate of cell proliferation in culture were similar for all the cell variants. Cytoskeletal visualization by immunofluorescence staining revealed that the tumorigenic and metastatic cell lines expressed an altered organization of actin cables and a smaller number of vinculin‐containing focal contacts. Lactoperoxidase iodination of cell surface proteins showed the appearance of an Mγ 86,000 protein in the tumorigenic and metastatic cell variants. Analysis of cell‐surface glycoproteins demonstrated an increased sialylation of Mγ 66,000 and Mγ 62,000 glycoproteins in the transformed, tumorigenic and metastatic cell lines. This angiosarcoma tumor model system allows investigation of cellular characteristics which might be relevant to specific stages in tumor progression.
Interaction between two cellular subpopulations of a rat colonic carcinoma when inoculated to the syngeneic host
Authors: Caignard A. , Martin M. S. , Michel M. F. , Martin F. 
Published: February 2007

AbstractFrom a single, chemically‐induced rat colonic carcinoma, two subpopulations of tumor cells have been isolated. When injected into syngeneic rats, TR cells give rise to progressive tumors in most of the animals, whereas TS cells give rise to no tumors or to tumors which regress in less than 30 days. TS cells inhibit the growth of TR tumors, whether they are injected before TR cells or simultaneously, at a different site or mixed with the TR cells. Lymph nodes and spleen lymphocytes from animals having rejected TS tumors also inhibit TR‐cell growth. On the other hand, TS cells are able to give rise to progressive tumors when they are injected into rats bearing established TR tumors. Lymph nodes and spleen cells of TR tumorbearing rats are able to enhance TS cell growth. These results suggest that subpopulations of cancer cells contained in the same tumor interact with each other through their effect on the host immune system. The growth of a whole tumor probably depends not only on the growth potential of each constituent subpopulation, but also on the interaction between the subpopulations themselves.
Influence of reproductne history on age at diagnosis of breast cancer and prognosis
Authors: Wang D. Y. , Allen D. S. , Bulbrook R. D. , Rubens R. D. , Millis R. R. , Chaudary M. A. , Hayward J. L. 
Published: February 2007

AbstractThe effect on age at breast cancer diagnosis of age at menarche, age at first baby, parity and age at menopause has been determined for 739 unselected patients diagnosed between 1975 and 1980 as having operable breast cancer. Age at diagnosis was significantly and positively associated with ages at menarche, first baby and menopause. The average number of children significantly declined with increasing age at diagnosis. This was largely due to a change in the proportion of patients who were nulliparous (15% in women aged 41‐50 years compared to about 30% in those over 60 years). A group of 1,989 normal women whose reproductive history was also collected between 1975 and 1980 showed similar trends between age and age at menarche, age at first baby and parity as the patients. Thus it appears that these reproductive parameters do not alter the time of onset of breast cancer but could be explained by temporal changes in reproductive patterns. There was no significant correlation between age at menopause and age at diagnosis for patients whose age at menopause was no more than 54 years and age at diagnosis no less than 55 years, respectively. It is therefore unlikely that age at menopause affects age at diagnosis. Postmenopausal patients with 3 or more children had a significantly shorter disease free interval and lower survival rate than those with less children. None of the other parameters was associated with prognosis.
Partial purification and characterization of a vascular permeability factor secreted by a human colon adenocarcinoma cell line
Authors: Lobb Roy R. , Key Marc E. , Alderman Edward M. , Fett James W. 
Published: February 2007

AbstractThe established human colon adenocarcinoma cell line, HT‐29, secretes a vascular permeability factor (VPF) in vitro. The factor has been purified from serum‐free conditioned medium by acidification, cation‐exchange, and reverse‐phase and anion‐exchange high‐performance liquid chromatography. The VPF is a non‐glycosylated acidic protein of apparent molecular weight 45,000, and is at least 4 orders of magnitude more potent than histamine as an inducer of vascular permeability. Its biological activity is unaffected by soybean trypsin inhibitor or by inhibitors of histamine, kinins, prostaglandins, or acid proteases. The VPF induces vascular permeability within minutes, which suggests a direct effect on the endothelial cell.
Structure‐activity relationship in the induction of Epstein‐Barr virus by teleocidin derivatives
Authors: Koshimizu Koichi , Hagiwara Nobuyuki , Irie Kazuhiro , Ito Yohei , Tokuda Harukuni , Murao Sawao , Hayashi Hideo 
Published: February 2007

AbstractNew derivatives of (‐)‐indolactam V (Fig. I), which have the basic ring‐structure of teleocidins without the monoterpenoid moiety, were prepared and their Epstein‐Barr virus early antigen (EBV‐EA)‐inducing activity was tested. (‐)‐14‐O‐Alkyl indolactam Vs (2 and 3) showed little induction of EBV‐EA, while (‐)‐14‐dehydroxyindolactam V (4) and (‐)‐14‐chloroindolactam V (5) proved to be potent EBV‐EA inducers, though their activities (EC50) were about 10 times weaker than that of (‐)‐indolactam V (1). These results indicate that the hydroxyl group at C‐14 is not indispensable for EBV‐EA induction and can be replaced. The activities (EC50) of (‐)‐I‐N‐methyl, (‐)‐I‐N‐ethyl, and (‐)‐I‐N‐butyl indolactam V (10, 11, and 12) were about 5 times weaker than that of (‐)‐indolactam V (1), while (‐)‐I‐N‐hexyl and (‐)‐I‐N‐octyl indolactam V (13 and 14) were even less active, suggesting that the free imino group of the indole ring in (‐)‐indolactam V (1) plays an important role in the activity, and that the activity cannot be enhanced by alkylation at the N‐I position of (‐)‐indolactam V (1).
Tiadenol‐mediated induction of peroxisomal enzymes in cultured C3H/10T1/2CL8 cells and in chemically transformed C3H/10T1/2 MCA16 cells
Authors: Berge Rolf K. , Lillehaug Johan R. 
Published: February 2007

AbstractThe levels of peroxisomal enzyme activities in cultured C3H/10T1/2 CL8 cells and in chemically transformed C3H/10T1/2 MCA16 cells were studied after treatment with tiadenol and niadenate, two hypolipidemic drugs which are both carcinogenic and cause peroxisome proliferation in vivo. Administration of these peroxisome proliferators to the cells resulted in large increases in specific palmitoyl‐CoA hydrolase, carnitine acetyl‐transferase, and catalase activities. A reproducible induction of cyanide‐insensitive palmitoyl‐CoA oxidative activity was observed 5 and 9 days after initiation of tiadenol treatment. Basal activity was 0.16 nmole/min/mg protein compared to 0.95 nmole/min/mg protein in cells treated with 18 μM tiadenol (cytotoxicity of about 25%) for 9 days. The enzyme activities were more increased in the transformed MCA 16 cells than in the non‐transformed cells and the order of increase in enzyme activities was: niadenate > tiadenol. In non‐transformed cells, the specific activity of palmitoyl‐CoA hydrolase was enhanced approximately 2.1‐fold within 4 days after tiadenol treatment. During this time period the enzyme activity was constant in untreated cells, but decreased during longer incubation periods. The enhancement of palmitoyl‐CoA hydrolase, carnitine acetyl‐transferase and catalase activities was dose‐related over a concentration range of 2 to 20 μM tiadenol, depending on the enzyme assayed. Tiadenol concentrations above 10 μM were increasingly cytotoxic, while 18 μM niadenate had no toxic effect on the C3H/10T1/2 C18 cells. Moreover, the stimulation of the 3 enzyme activities by the peroxisome proliferators were inhibited by cycloheximide. Neither of the two cell lines contained any appreciable urate oxidase activity. The responses of these cells to hypolipidemic drugs show that they constitute a useful system for studies on the role of peroxisomes in lipid metabolism and the relationship between hypolipidemic activity and carcinogenic potential of these drugs.
Lung colonization and metastasis by disseminated b16 melanoma cells: H‐2 associated control at the level of the host and the tumor cell
Authors: Taniguchi Kazuto , Käarre Klas , Klein George 
Published: February 2007

AbstractWe have studied the experimental metastasis of H‐2+ and H‐2− melanoma sublines in H‐2b/b and H‐2a/b hosts by enumerating pulmonary colonies 20–50 days after i.v. inoculation of tumor cells. In H‐2b/b hosts, the H‐2+ „B 16‐S”︁ cells gave rise to a moderate number of metastatic colonies (mean: 6.3 ± 6). The „BL 16‐L”︁ sublines that had lost the expression of MHC class I antigens, according to FACS‐analysis and quantitative absorption tests, gave no metastases under the same conditions. Pretreatment of the H‐2+ met+ B 16‐S with interferons (beta or alpha + beta) increased their H‐2 antigen expression and the number of metastatic colonies (mean: 25 ± 16). Interferon pretreatment of B16‐L cells partially restored their H‐2b expression and induced them to form a small number of metastatic colonies. The reduction in pulmonary colonization by the H‐2 negative B16‐L cells could be attributed to their rapid elimination by natural killer cells, already observed within 24 hr of inoculation of radiolabelled cells. H‐2− B16‐L cells were more susceptible than H‐2+ B16‐S cells to in vitro lysis by poly I:C‐treated splenocytes, and they acquired full metastatic abilities if the hosts were treated with anti‐asialo GM‐I serum. In H‐2a/b heterozygous hosts, the H‐2+ B16‐S cells also failed to metastasize. Reduced pulmonary colonization was evident by 24 hr after injection in comparison with H‐2b/b hosts, and could be reversed by anti‐asialo GM‐I treatment of the hosts. In vitro, H‐2a/b splenocytes were more cytotoxic to the B16 cells than syngeneic effectors. The results are discussed in relation to a recent hypothesis on a surveillance mechanism for elimination of cells on the basis of their lack (or insufficient expression) of host MHC genes.
A potential role for the extracellular matrix glycoprotein laminin in macrophage‐tumor‐cell interactions
Authors: Huard Thomas K. , Baney Jill L. , Wood James F. B. , Wicha Max S. 
Published: February 2007

AbstractAlthough cell surface molecules are thought to be involved in macrophage (MO)‐tumor‐cell recognition, the nature of these molecules remains unknown. In this study we have shown that the glycoprotein laminin may facilitate macrophage‐tumor‐cell binding. Macrophage binding to tumor cells was assessed by measuring the adherence of radiolabelled 3‐MCA2 induced malignant fibrosarcoma cells to syngeneic peritoneal MOs. Addition of exogenous laminin promoted the binding of a weakly metastatic subline of these tumor cells by 31‐68%. These weakly metastatic tumor cells express negligible endogenous cell‐surface laminin but display specific cell‐surface receptors for binding soluble laminin. Exogenous laminin promoted MO binding of these tumor cells whether it was present during the assay or whether the tumor cells were pretreated with the laminin. This increase in binding was blocked by anti‐laminin antibody. In contrast, MO binding of a strongly metastatic variant of the same tumor was not enhanced by the addition of exogenous laminin. This highly malignant fibrosarcoma line already expressed endogenous cell‐surface laminin. Since the MOs were found to specifically bind <125>I‐laminin, the interaction between laminin‐bearing tumor cells and MOs may be mediated via a specific MO plasma membrane receptor. Thus, the expression of cell surface laminin and its receptors on both tumor cells and MOs may provide a mechanism for promoting MO‐tumor‐cell binding.
Detection of a retrovirus‐related glycoprotein in immune complexes from patients with hematopoietic disorders
Authors: Jacquemin P. C. , Striuckmans P. 
Published: February 2007

AbstractHuman sera from normal and leukemic individuals were found to contain various amounts of an antigen with determinants related to pI5E and reverse transcriptase of retroviruses. Using a specific monoclonal antibody and the immuno‐affinity purified antigen, we surveyed a series of sera and plasmas from normal individuals and hematological patients by competition radioimmunoassays and binding assays directed against this specific protein. It was detected in all the samples at various levels. The level of this 74‐kd glycoprotein appeared to be related to a stimulation of the hematopoietic system. No free antibodies were found that could recognize the labelled purified antigen. Only immune complexes prepared from the blood of some hematological patients contained the specific antigen, but complexes prepared from the blood of normal individuals did not.
Prospective studies on nasopharyngeal carcinoma in epstein‐barr virus IgA/VCA antibody‐positne persons in Wuzhou city, china
Authors: Zeng Y. , Zhang L. G. , Wu Y. C. , Huang Y. S. , Huang N. Q. , Li J. Y. , Wang Y. B. , Jiang M. K. , Fang Z. , Meng N. N. 
Published: February 2007

AbstractA serological mass survey was carried out in Wuzhou City in 1980, 1,136 IgA/VCA‐positive persons being followed up for 4 years. Altogether 35 NPC cases were detected, of which 15 (43%) were in stage I and 17 (48.5%) in stage II, early cases (I + II) thus amounting to 91.5%. The detection rate of early cases was 2.9 times higher than in our outpatient clinic. IgA/VCA antibody could be detected 16–41 months prior to clinical diagnosis of NPC. We conclude that, if IgA/VCA‐positive individuals are examined routinely once a year, NPC can be detected in the early stages of evolution. The annual detection rate of NPC in IgA/VCA antibody‐positive individuals was 31.7 times higher than that of the annual incidence of NPC in the general population in the same age group, while during the 4‐year follow‐up period the incidence was 7.5 times higher than in the general population for the same age group. These results further indicate that EB virus plays an important role in the development of NPC, and that serological screening and follow‐up studies are valuable for the early detection of NPC.
HTLV‐I is endemic in Southern Italy: Detection of the first infectious cluster in a white population
Authors: Manzari Vittorio , Gradilone Angela , Barillari Giovanni , Zani Massimo , Collalti Enrico , Frati Luigi , Pandolfi Franco , De Rossi Giulio , Liso Vincenzo , Babbo Pasquale , Guroff Marjorie Robert 
Published: February 2007

AbstractHuman T‐cell leukemia virus (HTLV‐I) infection is observed among black and Japanese populations in well‐delimited endemic spots in association with a high incidence of adult T‐cell leukemia (ATL).We present evidence of HTLV‐I infection in two ATL patients from southeastern Italy who have not travelled and who have no known relations abroad, and in 8% of non‐leukemic controls from the same area. Thus, populations exhibiting HTLV‐I infection appear more widespread than supposed up to now.
Expression of burkitt lymphoma‐associated antigen (defined by the monoclonal antibody 38.13) on both normal and malignant germinal‐centre B cells
Authors: Murray L. J. , Habeshaw J. A. , Wiels J. , Greaves M. F. 
Published: February 2007

AbstractMonoclonal antibody 38.13 was raised against the Burkitt lymphoma cell line Daudi and has been previously shown to recognize a globotriaosylceramide (pk) determinant expressed selectively on Burkitt and a few other B‐cell lymphomas. We report here that 38.13 binds to a subset of normal B lymphocytes in germinal centres of secondary lymphoid follicles as well as to blood vessels. In malignant populations equivalent to various developmental compartments of the B‐cell lineage, only those with a follicular phenotype express the pk determinant at detectable levels. The expression of this tumour‐associated antigen, in common with many others, is therefore regulated in concert with cell lineage and maturation status.
Multiple epitopes on a human breast‐carcinoma‐associated antigen
Authors: Price M. R. , Edwards S. , Owainati A. , Bullock J. E. , Ferry B. , Robins R. A. , Baldwin R. W. 
Published: February 2007

AbstractThe monoclonal antibody (MAb) NCRC‐11 identifies an epitope expressed variably in human breast cancer. The degree of expression of this epitope in primary operable tumours is closely related to the subsequent clinical course of the disease (Ellis et al., 1985). The target antigen for NeRC‐11 was isolated from subcellular membranes of breast carcinomas and purified by immunoadsorbent chromatography. NCRC‐11 epitopes were expressed upon a large glycoprotein of more than 400 kd. This material was susceptible to degradation by pronase and papain and contained Nacetylglucosamine, as indicated by its binding to wheat‐germ agglutinin. The NCRC‐11‐defined antigen expressed epitopes for the anti‐human milk‐fat globule membrane antibodies HMFG‐1 and HMFG‐2, and other antibodies against epithelial membrane antigens (EMA, LICR‐LON‐M8). The reactivity of these antibodies with tumour membranes was also similar, but not identical, to that of the NCRC‐11 antibody. In competitive binding‐inhibition assays these antibodies partially inhibited the binding of 125I‐NCRC‐II antibody to antigen, suggesting that the epitopes involved are topographically closely associated. Sandwich immunoassays demonstrated that NCRC‐11 epitopes are likely to represent repeated structures of the NCRC‐11 antigen. The findings presented are interpreted as indicating that the NCRC‐11 antigen expresses a variety of epitopes which are associated with normal differentiation and malignant change.
Papillomavirus DNA in human tongue carcinomas
Authors: De Villiers E.‐M. , Weidauer H. , Otto H. , Zur Hausen H. 
Published: February 2007

AbstractSeven biopsies from different carcinomas of the tongue were analyzed for human papilloma virus (HPV) sequences by Southern blot analysis. After hybridization with various types of human papilloma viruses, 1 tumors were found to be positive. Whereas DNA from one tumor hybridized with HPY 2 DNA under conditions of high stringency, the 2 other positive biopsies contained HPV 16 DNA. All positive tumors revealed high copy numbers of the respective DNA. The cleavage pattern of the HPV‐2‐positive tumor differed from the established HPV 2 prototypes. Minor differences from the HPV 16 prototype were also noted in one of the HPV‐16‐positive tongue carcinomas.