bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional apoptosis profiling reveals vulnerabilities in T-cell large granular lymphocytic leukemia
Authors: Shumilov Evgenii , Mazzeo Paolo , Trautmann Marcel , Levien Lena , Menck Kerstin , Richter Katharina , Markus Katharina , Ries Lena , Haase Detlef , Oberle Elena , Berning Philipp , Hartmann Wolfgang , Ströbel Philipp , Kerkhoff Andrea , Lenz Georg , Wulf Gerald , Koch Raphael 
Published: February 2025

Abstract
T-cell large granular lymphocytic leukemia (T-LGLL) is a rare hematologic neoplasm characterized by clonal expansion of CD3 + cytotoxic T lymphocytes and a highly heterogeneous clinical course. Conventional therapy primarily includes immunosuppressive regimen. However, optimal front-line approaches still need to be defined and refractory disease remains a clinical challenge. Thus, we here aimed to explore functional dependencies of T-LGLL as a basis for personalized therapeutic strategies. We performed functional apoptosis profiling and ex vivo drug treatment in a series of 8 clinically and genetically characterized T-LGLL patients from two German University hospitals. Our series of patients underscored the clinical and genetic heterogeneity of the disease. Genetically, only 2 patients harbored a STAT3 mutation. To identify targetable anti-apoptotic mechanisms, we performed selective functional BH3 profiling on the patients’ CD8 + T-cells harboring the malignant T-LGLL cells versus the same patients’ normal CD4 + T-cells. CD8 + cells in 50% of the patients (4/8) demonstrated a dominant functional dependence on MCL-1 as compared to the same patients’ normal T-cells. Accordingly, CD8 + T-LGLL cells from patients with enhanced MCL1 dependence significantly responded to AZD-5991 ex vivo while no response was observed in the remaining samples lacking enhanced MCL-1 dependence. Across clinically and genetically heterogeneous cases of T-LGLL, functional apoptosis profiling identified patients with CD8 + T-LGLL cells harboring a dominant dependence on MCL-1 as a potential therapeutic target.
Hormone and HER2-receptor status in breast cancer: determination using sonographically guided core needle biopsy and correlation with excision specimen—a German single institution diagnostic accuracy study
Authors: Pruss Maximilian , Cieslik Jan-Philipp , Török Janet , Dobrowolski Jerome , Neubacher Melissa , Helbig Martina , Friebe Verena , Häberle Lena , Krawczyk Natalia , Borgmeier Felix , Fehm Tanja , Dietzel Frederic , Mohrmann Svjetlana 
Published: February 2025

Abstract

Background
Sonographically guided core needle biopsy (CNB) is a well-established tool for diagnosing breast lesions. Preoperative estrogen receptor (ER), progesterone receptor (PR), and HER2-receptor status are essential for a personalized treatment approach.

Objectives
We evaluated the concordance of the hormone- and HER2-receptor status between the CNB and the surgical specimen to determine the accuracy of the CNB as a diagnostic method.

Design
This is a non-interventional retrospective study analyzing breast cancer patients treated at the breast care center of the University Medical Center Duesseldorf between January 2002 and December 2005.

Methods
Patients with paired CNB and surgical specimens and a diagnosis of invasive breast cancer were included. ER, PR, and HER2 status were determined by immunohistochemistry (IHC). Patients with IHC 2+ results were further examined by fluorescence in situ hybridization (FISH). Concordance of receptor status was calculated using specificity, sensitivity, and negative and positive predictive values.

Results
We found a very good agreement between CNB and surgical specimens regarding receptor status. A total of 248 patients were analyzed. Concordance rates in cases of primary surgery for ER, PR, and HER2 were 92.9%, 92.9%, and 93%, respectively. In cases of neoadjuvant chemotherapy, the concordance rates for ER, PR, and HER2 were 100%, 87.5%, and 96%, respectively.

Conclusion
CNB demonstrated high diagnostic accuracy compared with surgical specimens regarding ER, PR, and HER2-receptor status. Our findings support the recommendation to use sonographically guided CNB as the initial diagnostic method for guiding tailored treatment plans.
New data on the life cycle of Nippostrongylus brasiliensis (Travassos, 1914) (Nematoda: Heligmosomidae): development of eggs and larval stages in the intestine of naturally infected Rattus norvegicus (Berkenhout, 1769)
Authors: Galán-Puchades María Teresa , Gómez-Samblás Mercedes , Trelis María , Osuna Antonio , Bueno-Marí Rubén , Sáez-Durán Sandra , Conn David Bruce , Fuentes Màrius V. 
Published: February 2025

Abstract

Nippostrongylus brasiliensis is an intestinal parasite of rodents with a worldwide distribution. Due to the similarities between its life cycle and that of the human hookworms Ancylostoma duodenale and Necator americanus, N. brasiliensis has been widely used as a model in experimental research. We provide new data on the embryonation and hatching processes of N. brasiliensis eggs, not in the soil, but in the large intestine of naturally infected Rattus norvegicus. The parasite was investigated in 109 rats trapped in urban and periurban areas of Valencia, Spain. Rats were preserved at − 20 °C. After thawing, all the rat organs were examined. The contents of the large intestine were analysed by the Midi-Parasep® technique. N. brasiliensis (adult prevalence 69.72%) was identified by morphological and molecular techniques. Parasite eggs were found in 88.16% of the sediments. In addition to unembryonated eggs, eggs in different stages of embryonation were encountered in 67 of the 76 contents (88.16%). Free larval stages (L1 and more developed larvae) were also found in 47 of the 76 (61.84%) sediments studied. Filariform larval stages of N. brasiliensis were also sporadically detected in the lungs of the infected rats. According to our results, the development of eggs and larvae in the intestine of naturally infected rats opens up the possibility of autoinfection in the natural life cycle of N. brasiliensis, which could explain the high burdens of adult stages frequently found in the small intestine of infected rats.
New comparative approach to multi-level thresholding: chaotically initialized adaptive meta-heuristic optimization methods
Authors: Serbet Fatmanur , Kaya Turgay 
Published: February 2025

Abstract
One method aimed at enhancing the performance of meta-heuristic optimization techniques is the incorporation of chaotic systems. Instead of irregular distributions in the search space, chaotic distributions are employed in the initial population of optimization algorithms to improve the efficiency of the search process. This approach enables search agents distributed in a chaotic manner to effectively explore the search space. The initial populations of both the well-established PSO algorithm and the enhanced WSO algorithm, which incorporates advanced search techniques, are distributed in the search space according to the characteristics of Logistic, Chebyshev, Circle, Sine, and Piecewise chaotic maps in this study. The original PSO and WSO algorithms, as well as the resulting chaotically initialized PSO and chaotically initialized WSO algorithms, were tested using 23 benchmark functions. Subsequently, the Otsu method was integrated into the tested optimization algorithms to obtain multi-level thresholding values. These algorithms were applied to five different test images with a manually determined number of thresholds. The results obtained were presented in the study and evaluated using statistical tests.
The nonlinear fast diffusion equation on smooth metric measure spaces: Hamilton-Souplet-Zhang estimates and a Ricci-Perelman super flow
Authors: Taheri Ali , Vahidifar Vahideh 
Published: February 2025

Abstract
This article presents new gradient estimates for positive solutions to the nonlinear fast diffusion equation on smooth metric measure spaces, involving the f-Laplacian. The gradient estimates of interest are of Hamilton-Souplet-Zhang or elliptic type and are established using different methods and techniques. Various implications, notably to parabolic Liouville type results and characterisation of ancient solutions are given. The problem is considered in the general setting where the metric and potential evolve under a super flow involving the Bakry-Émery m-Ricci curvature tensor. The curious interplay between geometry, nonlinearity, and evolution – and their intricate roles in the estimates and the maximum exponent range of fast diffusion – is at the core of the investigation.
Aldh2 and the tumor suppressor Trp53 play important roles in alcohol-induced squamous field cancerization
Authors: Kondo Yuki , Ohashi Shinya , Katada Chikatoshi , Nakai Yukie , Yamamoto Yoshihiro , Tamaoki Masashi , Kikuchi Osamu , Yamada Atsushi , Hirohashi Kenshiro , Mitani Yosuke , Kataoka Shigeki , Saito Tomoki , Vu Trang H. Nguyen , Kondo Tomohiro , Uneno Yu , Sunami Tomohiko , Yokoyama Akira , Matsubara Junichi , Matsuda Tomonari , Naganuma Seiji , Oryu Kohei , Flashner Samuel , Shimonosono Masataka , Nakagawa Hiroshi , Muto Manabu 
Published: February 2025

Abstract

Background
Field cancerization defined by multiple development of squamous cell carcinomas (SCCs) in upper aerodigestive tract was explained by excessive alcohol intake. A dysfunctional mitochondrial aldehyde dehydrogenase 2 (Aldh2) delays the clearance of acetaldehyde, a genotoxic alcohol metabolite, and increases SCC risks. TP53 plays key roles in squamous carcinogenesis. However, the mechanism of alcohol-mediated squamous field cancerization has not been clearly elucidated.

Methods
We developed a novel genetically engineered mouse strain KTPA
–/– (Krt5Cre

ERT2

; Trp53

loxp/loxp

; Aldh2
–/–) featuring Aldh2-loss concurrent with epithelial-specific Trp53 deletion. These mice were given 10%-EtOH, and we evaluated the development of squamous cell carcinogenesis histologically and genetically.

Results
Widespread multifocal rete ridges (RRs), characterized by downward growth of proliferative preneoplastic cells, were found only in Aldh2
+/– and Aldh2
–/– mice with keratin5-specific Trp53 deletion (KTPA
+/–
and KTPA

–/–
mice, respectively), and alcohol drinking apparently increased RR formation rate. SCC occurred only in KTPA

–/–
(Aldh2 loss/TP53 loss) mice with alcohol drinking (15/18: 83%). Total alcohol consumption volume was significantly higher in KTPA

–/–
(Aldh2 loss/TP53 loss) mice with SCCs than those without SCCs. Further, target sequence revealed the occurrence of genetic abnormalities including Trp53 mutations in the esophageal epithelium of Aldh2

–/–
mice with alcohol drinking, suggesting direct mutagenic effects of alcohol drinking to the esophageal epithelium.

Conclusion
This study provides for the first time the evidence that alcohol drinking, Aldh2 dysfunction and Trp53 loss cooperate in squamous field cancerization. Alcohol consumption volume affects the SCCs development, even in the same genotype.
Impacts of Compression on the Ground and Low-Lying Excited Doublet States of Plasma-Embedded Lithium Atom
Authors: Doma Salah , Roston Gamal , Ahmed Mostafa 
Published: February 2025

Abstract
The variational Monte Carlo method is employed to conduct a comprehensive investigation of the compressed ground and excited states of plasma-embedded lithium atom within impenetrable spherical boxes of varying radii. The study focuses on the low-lying excited doublet states 1

$$s^{{2}}$$

s
2

ns, 1

$$s^{{2}}n$$

s
2

n

p, and 1

$$s^{{2}}n$$

s
2

n

d, utilizing plasma potentials such as the screened Coulomb (SCP), exponential cosine screened Coulomb (ECSCP), and Hulthén potentials. Energy eigenvalues are determined using appropriate trial wave functions, which account for electron–electron repulsion and spin parts to adhere to the Pauli Exclusion Principle. Moreover, two factors related to the wave function of the compressed system and ECSCP model are considered. The results reveal an intriguing relative ordering for the lithium atom using the three plasma models, with many of the findings being significant contributions yet to be explored.
Systemic risk factors contributing to sialolithiasis: a big-data retrospective analysis
Authors: Jonas Ehud , Gillman Leon , Masri Daya , Rosenfeld Eli , Chaushu Gavriel , Avishai Gal 
Published: February 2025

Abstract

Aims
To investigate systemic risk factors contributing to the formation of sialolithiasis.

Methods
A retrospective big-data cohort study was conducted using data from Clalit HMO in Israel. Sialolithiasis patients were identified based on ICD-10 codes and matched with controls. Univariate and multivariable logistic regression analyses were performed to assess the relationship between systemic conditions and sialolithiasis. P-value < 0.05 was considered significant.

Results
5100 sialolithiasis cases were collected. The statistical analysis revealed that hypertension (OR = 1.14, 1.08–1.24, p < 0.001), dyslipidemia (OR = 1.33, 1.27–1.41, p < 0.001), nephrolithiasis (OR = 1.55, 1.42–1.63, p < 0.001) and cholelithiasis (OR = 1.22, 1.16–1.27, p < 0.001) were significantly correlated with sialolithiasis.

Conclusions
Systemic diseases, particularly dyslipidemia, play a role in the development of sialolithiasis. Further research is needed to explore the underlying mechanisms linking these conditions and to develop targeted strategies for the prevention of sialolithiasis.

Clinical relevance
This study highlights the potential interplay between systemic conditions and sialolithiasis. Recognizing these associations can inform clinical practice in understanding the pathogenesis of the disease, risk assessment, early diagnosis, and preventive measures.
Piano level laser therapy versus epidermal growth factor injection for painful myogenic temporomandibular disorder (a randomized clinical trial)
Authors: Qataya Passant Osama , Zaki Azza Mohamed , Amin Fatma , Swedan Ahmed , Elkafrawy Hagar 
Published: February 2025

Abstract

Objective
The aim of this clinical trial was to evaluate the effectiveness of Piano level laser therapy using Nd-YAG laser and intramuscular EGF injection in pain alleviation, function, and quality of life improvement in patients suffering from myogenic TMD.

Materials and methods
A randomized clinical trial was performed on 29 patients suffering from chronic painful myogenic TMD based on diagnostic criteria for temporomandibular disorders. Group I (n = 13patients) was treated using 1064 nm Nd-YAG Laser (4 sessions once/week). Group II (n = 14 patients) was treated by intramuscular injection of EGF. Pain using numerical rating score, pain free opening and unassisted maximum opening were measured at baseline, 7,14,21 days, 1 and 3 months. Quality of life using OHIP-14 was assessed at baseline, 1 and 3 months.

Results
Results showed that there was a significant pain reduction (P < 0.000) and increase in pain free opening (P < 0.0001) in both test groups. However, only group I showed a significant increase in maximum opening (P = 0.007). Quality of life significantly improved in both groups (P = 0.0001). There was no significant difference between the two treatments in pain scores, pain free opening, maximum opening nor quality of life.

Conclusion
Both treatment modalities offered effective and cost-effective non- to minimally invasive treatment options for myogenic TMD with no side effects.

Clinical relevance
Myogenic TMD forms a public health issue and is a common musculoskeletal problem causing pain and disability. The proposal of effective, non-invasive, and affordable treatment options can help solve this issue.
How bumblebees manage conflicting information seen on arrival and departure from flowers
Authors: Guiraud Marie-Geneviève , MaBouDi HaDi , Woodgate Joe , Bates Olivia K. , Rodriguez Oscar Ramos , Gallo Vince , Barron Andrew B. 
Published: February 2025

Abstract
Bees are flexible and adaptive learners, capable of learning stimuli seen on arrival and at departure from flowers where they have fed. This gives bees the potential to learn all information associated with a feeding event, but it also presents the challenge of managing information that is irrelevant, inconsistent, or conflicting. Here, we examined how presenting bumblebees with conflicting visual information before and after feeding influenced their learning rate and what they learned. Bees were trained to feeder stations mounted in front of a computer monitor. Visual stimuli were displayed behind each feeder station on the monitor. Positively reinforced stimuli (CS +) marked feeders offering sucrose solution. Negatively reinforced stimuli (CS−) marked feeders offering quinine solution. While alighted at the feeder station the stimuli were likely not visible to the bee. The “constant stimulus” training group saw the same stimulus throughout. For the “switched stimulus” training group, the CS + changed to the CS− during feeding. Learning was slower in the “switched stimulus” training group compared to the constant stimulus” group, but the training groups did not differ in their learning performance or the extent to which they generalised their learning. The information conflict in the “switched stimulus” group did not interfere with what had been learned. Differences between the “switched” and “constant stimulus” groups were greater for bees trained on a horizontal CS + than a vertical CS + suggesting bees differ in their processing of vertically and horizontally oriented stimuli. We discuss how bumblebees might resolve this type of information conflict so effectively, drawing on the known neurobiology of their visual learning system.
Socioeconomic impacts of wind farms in small and rural areas: a case study in North-eastern Spain
Authors: Almazán-Gómez M. Á. , Bielsa J. , Cazcarro I. , Langarita R. , Rodríguez-López G. 
Published: February 2025

Abstract
It is now clear that the adoption of renewable energies is of high importance in addressing climate change. In this sense, this work aims to contribute to the discussion on the net socioeconomic effects of renewable energy deployment on the places, especially rural areas, in which the plants are installed. To this end, we particularise the analysis to the case of wind power in an Aragonese county: Matarraña, in North-eastern Spain. We use the input–output approach to account for the local direct, indirect, and potentially induced effects in terms of value added and employment. Unlike previous versions of this methodology, the multiregional input–output method, with a high level of spatial and sectoral disaggregation, allows us to identify the place and the economic sector in which those effects occur. This high granularity of both the data and the model we use constitutes one of the main contributions of the paper. At the same time, we take into account the temporal sequence of the effects, an aspect that is essential in this case. Our results provide information on the issue of local acceptance of renewable energy deployments. Specifically, they suggest that, in the absence of other compensations, wind power does not seem to generate meaningful economic value or employment in the area (Matarraña county) where the windmills are installed.

Graphical abstract
Reduced repetition of Eurasian Chaffinch’s social calls near scree slopes in a Mediterranean forest
Authors: Pérez-González Javier , Hidalgo Toledo Sebastián P. , Guillén Ferreira José A. , Hidalgo de Trucios Sebastián J. , Rey-Gozalo Guillermo 
Published: February 2025

Abstract
Birds use songs and calls as communicative signals. The production of bird vocalizations depends on stimuli that prompt or deter communications. The quartzite scree slopes (pedrizas) in the Mediterranean forests of southwestern Spain might potentially engender conflict situation for bird communication. These open areas entail enhanced sound transmission and heightened predation. In this study, we investigate whether the presence of pedrizas influences the characteristics of Eurasian Chaffinch (Fringilla coelebs) social calls. We employed portable recorders to capture chaffinch vocalizations both within the forest and in the proximity to the pedrizas in the Mediterranean forest of the Monfragüe National Park (Spain). We found that the number of ‘chink’ repeats in chaffinch social calls was reduced in proximity to pedrizas compared to those recorded in the forest. This trend persisted across different conditions. Our finding lends support to the coexistence of stimuli in pedrizas that both prompt and deter bird communication. As a consequence, a conflict might arise, leading chaffinches to potentially respond by emitting social calls while simultaneously shortening the duration of vocalizations to minimize the risk of attracting undetected predators.
Long-Run Trends and Cycles in US House Prices
Authors: Caporale Guglielmo Maria , Gil-Alana Luis Alberiko 
Published: February 2025

Abstract
This paper analyses US nominal house prices at an annual frequency over the period from 1927 to 2022 by means of a very general time series model. This includes both a (linear and non-linear) deterministic and a stochastic component, with the latter allowing for fractional orders of integration at both the long-run and the cyclical frequencies. The results are heterogeneous depending on the model specification and on whether or not the series have been logged. Specifically, a linear model appears to be more appropriate for the logged data whilst a non-linear one appears to be a better fit for the original ones. Further, the order of integration at the zero or long-run frequency is much higher than at the cyclical one. The former is in fact around 1 in all specified models, which implies a high degree of persistence of this component. Finally, the order of integration of the cyclical structure implies that cycles have a periodicity of about 8 years, but it is almost insignificant in all cases.
Matrix Solutions of Biot’s Poroelasticity in Saturated Multilayered Media
Authors: Yue Zhongqi Quentin 
Published: February 2025

Abstract
This paper presents analytical formulations for systematically deriving the solutions of Biot’s poroelasticity in saturated multilayered media of either full-space or halfspace extents. The number of the saturated multilayer media is either

$n+2$

n
+
2

for full-space extent or

$n+1$

n
+
1

for halfspace extent, where

$n$

n

is a positive or zero integer. The applied loadings include the internal forces and liquid source for full-space and both internal and external loadings for halfspace region with eight cases of four boundary conditions. The mathematical tools for the formulations are classical and include the two-dimensional Fourier transform, the Hankel transform, Laplace transform as well as linear algebra. The solutions are expressed in matrix forms and each matrix is explicitly expressed with clear physical meaning and well-defined elements. The matrix solutions in the Fourier and Laplace transform domains are axially symmetric about the vertical axis. The internal and boundary conditions can be four-dimensional and the matrix solutions in the physical domain are also four-dimensional.