bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of age on vascularization during fracture repair
Authors: Lu Chuanyong , Hansen Erik , Sapozhnikova Anna , Hu Diane , Miclau Theodore , Marcucio Ralph S. 
Published: May 2008

AbstractAge affects fracture repair; however, the underlying mechanisms are not well understood. The goal of this study was to assess the effects that age has on vascularization during fracture healing. Tibial fractures were created in juvenile (4‐week‐old), middle‐aged (6‐month‐old), and elderly (18‐month‐old) mice. The length density and surface density of blood vessels within fracture calluses were analyzed using stereology at 7 days after fracture. The expression of molecules that regulate vascular invasion of the fracture callus was also compared among the three age groups by immunohistochemistry and in situ hybridization. At 7 days after fracture, juvenile mice had a higher surface density of blood vessels compared to the middle‐aged and elderly. Hypoxia‐inducible factor‐1α protein and transcripts of vascular endothelial growth factor were detected at 3 days postinjury in juvenile but not middle‐aged and elderly mice. Stronger Mmp‐9 and ‐13 expression was detected in fracture calluses at day 7 in the juvenile compared to the middle‐aged and elderly mice. At 21 days postfracture, expression of both Mmps was more robust in the elderly than juvenile and middle‐aged animals. These data indicate that age affects vascularization during fracture repair, and the changes we observed are directly correlated with altered expression of biochemical factors that regulate the process of angiogenesis. However, whether the increased vascularization is the cause or result of accelerated bone repair in juvenile animals remains unknown. Nonetheless, our results indicate that enhancing vascularization during fracture repair in the elderly may provide unique therapeutic opportunities. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1384–1389, 2008
Chemotaxis of human articular chondrocytes and mesenchymal stem cells
Authors: Mishima Yasunori , Lotz Martin 
Published: May 2008

AbstractMigration of chondrocytes and mesenchymal stem cells (MSCs) may be important in cartilage development, tissue response to injury, and in tissue engineering. This study analyzed growth factors and cytokines for their ability to induce migration of human articular chondrocytes and bone marrow‐derived mesenchymal stem cells in Boyden chamber assays.In human articular chondrocytes serum induced dose‐ and time‐dependent increases in cell migration. Among a series of growth factors and cytokines tested only PDGF induced a significant increase in cell migration. The PDGF isoforms AB and BB were more potent than AA. There was an aging‐related decline in the ability of chondrocytes to migrate in response to serum and PDGF. Human bone marrow MSC showed significant chemotaxis responses to several factors, including FBS, PDGF, VEGF, IGF‐1, IL‐8, BMP‐4, and BMP‐7. In summary, these results demonstrate that directed cell migration is inducible in human articular chondrocytes and MSC. PDGF is the most potent factor analyzed, and may be useful to promote tissue integration during cartilage repair or tissue engineering. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:1407–1412, 2008
Enrofloxacin‐imprinted monolithic columns synthesized using reversible addition‐fragmentation chain transfer polymerization
Authors: Liu Huina , Zhuang Xiaolei , Turson Mamat , Zhang Min , Dong Xiangchao 
Published: May 2008

AbstractMolecularly imprinted monolithic columns for selective separation of enrofloxacin were prepared by Reversible Addition‐Fragmentation Chain Transfer (RAFT)‐mediated radical polymerization. Different ratios of initiation system were used in the synthesis. The structures of the monoliths were characterized to study the relationship between the synthetic conditions and morphology of the monolithic material. The separation performance of the monoliths was evaluated by liquid chromatography. Under optimized synthetic conditions, a monolithic molecularly imprinted polymer (MIP) with high selectivity and improved column efficiency was obtained. The research has shown that RAFT polymerization provides more adjustable conditions for making monolithic materials with different morphologies. The results also demonstrated that homogeneous macro‐pore size distribution and large specific surface area are the key factors providing good separation ability and column efficiency for MIP monolithic structures.
Fast‐GC/HRMS to quantify the EU priority PAH
Authors: Ziegenhals Katja , Hübschmann Hans‐Joachim , Speer Karl , Jira Wolfgang 
Published: May 2008

AbstractFor the analysis of the 15 polycyclic aromatic hydrocarbons (PAH) classified as priority from the Scientific Committee on Food (SCF) and benzo[c]fluorene (BcL) assessed to be relevant by the Joint FAO/WHO Experts Committee on Food Additives (JECFA) in food, a sensitive analytical method is necessary. In the past, many methods were established for only the analysis of the 16 priority PAH recommended by the US Environmental Protection Agency (16 EPA‐PAH) but not for the 15 SCF‐PAH and BcL. Therefore, at the Max‐Rubner‐Institut, Federal Research Institute of Nutrition and Food in Kulmbach, Germany, an analytical method with a runtime of 72 min was developed. To shorten this runtime without a loss of separation, a Fast‐GC/high resolution MS (HRMS) method with a runtime of only 25 min using a TR‐50ms column (10 m×0.1 mm×0.1 μm) was created. The repeatability (n = 3) of spiked PAH concentrations in test‐matrix tea ranged from 0.1 to 11%. The analytical parameters LOD (0.01–0.02 μg/kg) and LOQ (0.03–0.06 μg/kg) also in the test‐matrix tea were determined. A good linearity for all PAH (R2 >0.99) and averaged recoveries from 75 to 117% in the concentration range of 1–20 μg/kg were achieved.
Analysis of corporate social responsibility in the service sector: does exist a strategic path?
Authors: Calabrese Armando , Lancioni Flavia 
Published: May 2008

AbstractThis paper proposes a strategic path managers might follow in order to optimise the outcome of the implementation of Corporate Social Responsibility (CSR) activities. As a starting point, we analysed the practice of CSR and its impact within a service industry, namely the Italian banking sector. Our aim was to understand the impact of CSR on the service company both externally and within the company itself and consequently our research was conducted on two different levels. The corporate level considers CSR as it is perceived by top management, and the operational level takes into account the perspectives of the front line employees and customers. Analysis at the corporate level was carried out by means of a thorough examination of the social reports and Internet sites of the service companies concerned, whereas the front line was studied by administrating a structured questionnaire, issued both to employees and to customers in a sample of bank branches.The research demonstrates that service companies are in fact implementing CSR initiatives and that stakeholders have a considerable interest in such initiatives. However, should the CSR initiatives be used as a tool solely to improve the brand equity rather than to improve relationships with their main stakeholders (employees and customers), then a boomerang effect is produced whereby the stakeholders pinpoint the CSR initiatives as one of the main reasons for their discontent with the service company. Although top managers invest in CSR initiatives in order to increase the satisfaction of the stakeholders, should the main stakeholders be unsatisfied with the management of the service company core business, the CSR initiatives might be rendered rather ineffective. As a result, the CSR initiatives may be a sign of underlying rift between top managers and the front line employees within the company, which in turn might also damage the relationship between bank and its customers. The implications of the findings in this paper provide a managerial tool for use in the implementation of CSR. This tool dictates a specific path to be followed which also requires precise timing for its success. Copyright © 2008 John Wiley & Sons, Ltd.
Novel progranulin mutation: Screening for PGRN mutations in a Portuguese series of FTD/CBS cases
Authors: Guerreiro Rita Joao , Santana Isabel , Bras Jose Miguel , Revesz Tamas , Rebelo Olinda , Ribeiro Maria Helena , Santiago Beatriz , Oliveira Catarina Resende , Singleton Andrew , Hardy John 
Published: May 2008

AbstractMutations in the progranulin (PGRN) gene were recently described as the cause of ubiquitin positive frontotemporal dementia (FTD) in many families. Different frequencies of these genetic changes have been reported in diverse populations leading us to determine if these mutations were a major cause of FTD in the Portuguese population. The entire coding sequence plus exon 0 of PGRN were sequenced in a consecutive series of 46 FTD/CBS Portuguese patients. Two mutations were found: a novel pathogenic insertion (p.Gln300GlnfsX61) and a previously described point variant (p.T182M) of unclear pathogenicity. Pathogenic mutations in the PGRN gene were found in one of the 36 probands studied (3% of the probands in our series) who had a corticobasal syndrome presentation, indicating that in the Portuguese population, mutations in this gene are not a major cause of FTD. © 2008 Movement Disorder Society
Polypropylene fibers containing dispersed clays having improved fire performance. Part II: characterization of fibers and fabrics from PP–nanoclay blends
Authors: Smart Gillian , Kandola Baljinder K. , Horrocks A. Richard , Nazaré Shonali , Marney Donavan 
Published: May 2008

AbstractIn this second part of a series of studies on polypropylene (PP) fibers having improved fire performance and containing nanodispersed clays we build on the observations of the first part of this work (published in Journal of Applied Polymer Science), where the need to improve dispersion of nanoclays is the key to achieve significant improvement. This paper attempts to address this issue by reporting studies of the use of several different functionalized clays in the presence and absence of compatibilizing PP copolymers and also to which the flame retardant (FR), ammonium polyphosphate (APP) has been introduced. Compounded polymer samples have been characterized using X‐ray diffraction (XRD), optical, and scanning electron microscopes, and their melt flow indices (MFI). These compounded polymers have been successfully extruded into filaments using a single screw extruder and then knitted into fabric samples. Filament physical properties are reported together with burning behaviors of fabric samples using limiting oxygen index (LOI) and flame spread tests. Copyright © 2008 John Wiley & Sons, Ltd.
Microwave‐enhanced rapid synthesis of organosoluble polyamides based on 5‐(3‐acetoxynaphthoylamino)‐isophthalic acid
Authors: Mallakpour Shadpour , Rafiee Zahra 
Published: May 2008

AbstractA series of photoactive polyamides (PAs) containing acetoxynaphthalamide side chain with inherent viscosities of 0.27–0.56 dl g−1 were prepared by the direct polycondensation reaction of the 5‐(3‐acetoxynaphthoylamino)isophthalic acid with various commercially available diamines by means of triphenyl phosphite (TPP) and pyridine (Py) in the presence of calcium chloride and N‐methyl‐2‐pyrrolidone (NMP) under microwave irradiation and conventional heating conditions. Most of the resulting PAs are soluble in strong polar solvents such as N,N‐dimethylformamide (DMF), N,N‐dimethylacetamide, and NMP. Thermo‐gravimetric analysis (TGA) showed that polymers are thermally stable, 10% weight loss temperatures in excess of 320 and 378°C, and char yields at 600°C in nitrogen higher than 60%. These macromolecules exhibited maximum UV‐Vis absorption at 265 and 300 nm in a DMF solution. Their photoluminescence in the DMF solution demonstrated fluorescence emission maxima around 361 and 427 nm for all of the PAs. Copyright © 2008 John Wiley & Sons, Ltd.
The relationship of parental overprotection, perceived vulnerability, and parenting stress to behavioral, emotional, and social adjustment in children with cancer
Authors: Colletti Christina J.M. , Wolfe‐Christensen Cortney , Carpentier Melissa Y. , Page Melanie C. , McNall‐Knapp René Y. , Meyer William H. , Chaney John M. , Mullins Larry L. 
Published: May 2008

AbstractBackgroundTo examine the relationship of self‐reported parental overprotection, perceived child vulnerability, and parenting stress to parent‐reported behavioral, emotional, and social adjustment of children currently on treatment for cancer.ProcedureParents of 62 children (34 boys, 28 girls) currently on treatment for cancer were recruited from an outpatient pediatric cancer clinic. Children ranged in age from 2 to 12 years; age at diagnosis ranged from 1.33 to 11.83 years.ResultsHigher levels of parenting stress, but not parental overprotection or perceived child vulnerability, were associated with poorer behavioral and social adjustment. Higher levels of perceived child vulnerability and parenting stress, but not parental overprotection, were independently associated with poorer emotional adjustment.ConclusionsSpecific parenting variables appear to be related to specific adjustment outcomes in children with cancer. Longitudinal follow‐up of these children is necessary to determine the developmental trajectory of parent variables and long‐term child outcomes. Pediatr Blood Cancer 2008;51:269–274. © 2008 Wiley‐Liss, Inc.
Imaging guidelines for children with Ewing sarcoma and osteosarcoma: A report from the Children's Oncology Group Bone Tumor Committee
Authors: Meyer James S. , Nadel Helen R. , Marina Neyssa , Womer Richard B. , Brown Kenneth L. B. , Eary J.F. , Gorlick Richard , Grier Holcombe E. , Randall R. Lor , Lawlor Elizabeth R. , Lessnick Stephen L. , Schomberg Paula J. , Kailo Mark D. 
Published: May 2008

AbstractThe Children's Oncology Group (COG) is a multi‐institutional cooperative group dedicated to childhood cancer research that has helped to increase the survival of children with cancer through clinical trials. These clinical trials include a standardized regimen of imaging examinations performed prior to, during, and following therapy. This article presents imaging guidelines developed by a multidisciplinary group from the COG Bone Tumor Committee. These guidelines provide both required and recommended studies. Recommended examinations may become required in the future. These guidelines should be considered a work in progress that will evolve with advances in imaging and childhood cancer research. Pediatr Blood Cancer 2008;51:163–170. © 2008 Wiley‐Liss, Inc.
‘I have a dream’: A process for visioning in practice development
Authors: Boomer Christine , Collin Ita , McCormack Brendan 
Published: May 2008

AbstractThe literature on practice development (PD) stresses the importance of having a ‘vision’, but provides little assistance on methodologies to develop one. This article describes in detail the process used to develop a shared vision in the context of a PD programme for clinical leaders in two Health and Social Services and Public Safety Trusts in Northern Ireland. Having a shared vision is seen as an essential foundation stone in the development of practice and in transformational cultures. The process used took the form of four distinct stages: preparation of participants for the visioning workshop; the first visioning workshop; visioning led by/facilitated by the programme participants; and the second visioning workshop. This work provided the foundation for development work in the subsequent cycles of the PD programme. Copyright © 2008 John Wiley & Sons, Ltd
Comparative study of electrostatic binding vs. complexation of Cu2+ ions with water‐soluble polymers containing styrene sulphonic acid and/or maleic acid units or their sodium salt forms
Authors: Oikonomou Eudokia K. , Bokias Georgios , Kallitsis Joannis K. 
Published: May 2008

AbstractThe interaction of Cu2+ ions with the homopolymer poly(styrene sulfonic acid) (PSSH), as well as with the copolymers of maleic acid (MAc) with styrene sulfonic acid (SSH) or vinyl acetate (VAc), was investigated in dilute aqueous solution through turbidimetry, potentiometry, viscometry, and spectrophotometry in the visible region. Cu2+ ions were introduced either through neutralization with Cu(OH)2 of the acid form of the (co)polymers (PSSH, P(SSH‐co‐MAc) and P(VAc‐co‐MAc)) or through mixing of the sodium salt form of the (co)polymers (PSSNa, P(SSNa‐co‐MANa) and P(VAc‐co‐MANa)) with CuSO4. Turbidimetry, potentiometry, and spectrophotometry revealed that the first carboxylic group of MAc or both carboxylate groups of MANa are involved in the complexation with Cu2+ ions when neutralization with Cu(OH)2 or mixing with CuSO4 are applied, respectively. The increased values of the reduced viscosity observed mainly at the first stages of neutralization of P(VAc‐co‐MAc) with Cu(OH)2 indicate that interchain polymer‐Cu2+ complexation takes possibly place. Finally, the spectrophotometric behavior observed upon neutralization of P(SSH‐co‐MAc) with Cu(OH)2 or mixing of P(SSNa‐co‐MANa) with CuSO4 revealed that the strength of counterion binding by the sulfonate groups is, in fact, comparable with the complexation of Cu2+ ions with the carboxylate groups of MAc. © 2008 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 1149–1158, 2008
Charge transport studies in electroluminescent biphenylyl substituted PPV derivatives using time‐of‐flight photoconductivity method
Authors: Vijila Chellappan , Ging Meng Ng , Zhi Kuan Chen , Furong Zhu , Soo Jin Chua 
Published: May 2008

AbstractThe charge transport properties in a series of electroluminescent biphenylyl substituted PPV derivatives, poly[(2‐(3′‐(p‐2″‐ethylhexyloxyphenyl)phenyl)‐1,4‐phenylenevinylene) (MP‐PPV), poly[2‐(2′‐phenyl‐4′,5′‐bis(3″‐methylbutoxy))phenyl‐1,4‐phenylene vinylene] (BP‐PPV) and poly{[2‐(4′,5′‐bis(3″‐methylbutoxy)‐2′‐phenyl)phenyl‐1,4‐phenylene‐vinylene]‐co‐(9,9‐dioctyl‐2,7‐fluorenylene‐vinylene)} (BPPPV‐PF), have been studied using a time of flight (TOF) photoconductivity technique. The TOF transients for holes in these polymers were weakly dispersive in nature with a mobility of the order of ∼10−5cm2/V s at room temperature. The temperature and field dependence of charge mobility has been studied and analyzed using the disorder formalisms (Bässler's Gaussian disorder model (GDM) and correlated disorder model (CDM)). The charge transport parameters, such as mobility prefactor, energetic disorder, positional disorder, and the average intersite distance, were estimated from the fit and correlated to the molecular structure of the polymer. The microscopic charge transport parameters derived for these polymers indicate that these biphenylyl substituted PPV derivatives have very good chemical purity and structural regularity, which is similar to the reported values for structurally related fully conjugated polymers with high chemical purity. © 2008 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 1159–1166, 2008
Gene expression changes in a transgenic mouse model overexpressing human wildtype and mutant torsinA
Authors: Grundmann Kathrin , Hübener Jeannette , Häbig Karina , Reischmann Bettina , Poths Sven , Hauser Till‐Karsten , Magg Janine , Riess Olaf , Bonin Michael , Nguyen Huu Phuc 
Published: May 2008

AbstractPrimary torsion dystonia is an autosomal‐dominantly inherited, neurodevelopmental movement disorder caused by a GAG deletion (ΔGAG) in the DYT1 gene, encoding torsinA. This mutation is responsible for approximately 70% of cases of early‐onset primary torsion dystonia. The function of wildtype torsinA is still unknown, and it is unsolved how the deletion in the DYT1 gene contributes to the development of the disease. To better understand the molecular processes involved in torsinA pathology, we used genome‐wide oligonucleotide microarrays to characterize gene expression patterns in the striatum of mouse models overexpressing the human wildtype and mutant torsinA. By this approach we were able to detect gene expression changes that seem to be specific for torsinA pathology. We found an impact of torsinA, independent from genotype, on vesicle trafficking, exocytosis, and neurotransmitter release in our mouse model. In addition, we were able to identify several new pathways and processes involved in the development of the nervous system that are affected by wildtype and mutant torsinA. Furthermore, we have striking evidence from our gene expression data that glutamate receptor mediated synaptic plasticity in the striatum is the affected underlying cellular process for impaired motor learning in human ΔGAG torsinA transgenic mice.
Heat‐shock protein‐27, ‐70 and peroxiredoxin–II show molecular chaperone function in sickle red cells: Evidence from transgenic sickle cell mouse model
Authors: Biondani Andrea , Turrini Franco , Carta Franco , Matté Alessandro , Filippini Alida , Siciliano Angela , Beuzard Yves , De Franceschi Lucia 
Published: May 2008

AbstractSickle cell disease (SCD) is an autosomal recessive genetic red cell disorder characterized by the production of a defective form of hemoglobin, hemoglobin‐S, that is worldwide‐distributed. The acute clinical manifestations of SCD are related to hemoglobin cyclic‐polymerization and to the generation of rigid, dense red blood cells (RBCs). We studied RBCs membrane proteome from human sickle RBCs, fractioned according to density compared to normal RBCs. 2‐DE followed by MS analysis was carried out. We identified 65 proteins differently expressed, divided into five major clusters according to their functions: (i) membrane‐cytoskeleton proteins; (ii) metabolic enzymes; (iii) ubiquitin‐proteasome‐system; (iv) flotillins; (v) chaperones. HSP27, HSP70 and peroxiredoxin‐II (Prx‐II) showed the most relevant changes. They were differently recruited to sickle RBCs membrane in response to in vitro hypoxia. Potential markers were then validated in a transgenic‐mouse model for SCD, the SAD mice, exposed to hypoxia mimicking acute SCD vaso‐occlusive‐crisis (VOCs); we found that HSP70 and HSP27 bound to RBCs membrane respectively after 12 h and 48 h of hypoxia, while Prx‐II membrane binding was modulated during hypoxia. Our data indicate that HSP27 and HSP70 play a novel role as RBCs membrane protein protectors and as possibly new markers of severity of RBCs membrane damage during acute VOCs.
Identification of proteins in human substantia nigra
Authors: Kitsou Efstathia , Pan Sheng , Zhang JianPeng , Shi Min , Zabeti Aram , Dickson Dennis W. , Albin Roger , Gearing Marla , Kashima Daniel T. , Wang Yan , Beyer Richard P. , Zhou Yong , Pan Catherine , Caudle W. Michael , Zhang Jing 
Published: May 2008

AbstractCharacterization of the human brain proteome is a critical area of research. While examination of the human cortex has provided some insight, very little is known about the proteome of the human midbrain, which demonstrates substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) in Parkinson's disease (PD). Therefore, characterization of this region is essential to a better understanding of the pathogenesis of PD. This dataset paper reports two separate studies, where human SNpc was collected from PD and control subjects and 1263 proteins were identified using MALDI‐TOF/TOF as well as linear ion trap MS platforms. With gene ontology analysis, the proteins were categorized according to their biological processes, as well as cellular components. These data were also compared with previous proteomic characterization of the human frontal and temporal cortex, and cerebrospinal fluid to establish shared proteins of relevance. The present dataset is the most extensive survey of the human SNpc proteome, to date. Further characterization of the SNpc proteome will significantly facilitate our understanding of the function and expression of proteins involved in PD, as well as provide potential proteins that may be utilized as biomarkers.