bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex-Mismatching in Isolated Heart Transplant Confers No Postoperative Risk
Authors: Dale Reid , Leipzig Matt , Bahatyrevich Nataliya , Pines Katharine , Chen Qiudong , Teuteberg Jeffrey , Woo Y. Joseph , Currie Maria 
Published: February 2024

AbstractIntroductionFor heart transplantation, optimal donor-recipient matching is an important factor in the ongoing development of the United Network for Organ Sharing (UNOS) continuous distribution framework. Donor-recipient sex-mismatch has decreased since the 1990s, but this may be related to the risk posed by size mismatching, particularly when donor hearts are undersized. Thus, the impact of sex-mismatching, controlling for other factors including size mismatch, is uncertain.MethodsAdult first-time, isolated heart transplant patients from the UNOS database between October 1, 1987 and December 31, 2022 were analyzed. Cohorts were separated into male and female recipients. Propensity score matching on known preoperative risk factors was performed. Equivalence testing via Two One-Sided Testing (TOST) was performed to assess between-arm equivalence in postoperative outcomes. Survival differences were measured by the between-arm ratio of Restricted Mean Survival Time and binary outcome differences by the Odds Ratio (OR).ResultsIn the propensity matched cohort, we found significant equivalence between arms in both male (TOST P<0.001) and female (TOST P<0.001) recipients for overall survival at all temporal endpoints, postoperative treatment for rejection within one year, and pre-discharge dialysis.ConclusionsSex-mismatch in isolated heart transplantation confers no additional risk to postoperative outcomes when controlling for other factors, including size mismatch. Consequently, sex-mismatch should not factor into individual assessments of organ acceptance or be incorporated into any national organ allocation policy. Increasing the acceptance of sex-mismatched donors has the potential to expand the donor pool and increase female donor utilization.
Activity-dependent regulation of vascular cholesterol metabolism acts as a negative feedback mechanism for neurovascular coupling
Authors: Profaci CP , Foreman KL , Spieth L , Coelho-Santos V , Berghoff SA , Fontaine JT , Jeffrey DA , Palecek SP , Shusta EV , Saher G , Shih AY , Dabertrand F , Daneman R 
Published: February 2024

SummaryBrain function is dependent on a continuous supply of bloodborne oxygen and nutrients. Because neurons require a greater supply of oxygen and nutrients when active, there is increased local blood flow following neuronal activity. The underlying mechanisms of this hyperemia are termed neurovascular coupling (NVC). Many complex processes contribute to NVC, and there is still much unknown about how vascular physiology adapts to changes in neuronal activity and blood flow. Here we show that neuronal activity increases brain endothelial expression of genes related to cholesterol synthesis and uptakein vivo, and that shear stress is sufficient for upregulation of these genesin vitro. We previously found that treatment with PLX5622 induces upregulation of the same cassette of cholesterol-related genes in brain endothelial cells. In the present study, we find that increasing brain endothelial cholesterol synthesis and/or uptake, either with PLX5622 or targeted AAV-mediated expression of LDLR, inhibits brain arteriole dilation in response to capillary K+stimulation, and this deficit is rescued by cholesterol depletion. Together, these data suggest that neuronal activity regulates brain endothelial cholesterol, which in turn blocks endothelial retrograde signaling and vasodilation, thus acting as a negative feedback mechanism for NVC.
The impact ofBacillus coagulansX3 on available nitrogen content, bacterial community composition, and nitrogen functional gene levels when composting cattle manure
Authors: Liu Biao , Chen Wei , Wang Zhen , Guo Zhaohui , Li Yongmei , Xu Lijuan , Wu Minxi , Yin Hongmei 
Published: February 2024

AbstractThis study was designed to probe available nitrogen levels, bacterial community composition, and the levels of nitrogen functional genes present when composting cattle manure with or without the addition ofBacillus coagulansX3. Bacterial supplementation was associated with the prolongation of the thermophilic stage and improved maturity of the resultant compost. At the maturity stage, samples to whichB. coagulansX3 had been added exhibited significant increases in ammonium nitrogen, nitrate nitrogen, and total nitrogen levels. The dominant bacterial phyla observed in these composting samples were Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteriota, and Chloroflexi.B. coagulansX3 addition resulted in significant increases in relative Firmicutes abundance during the thermophilic and cooling stages, while also increasingamoAandnosZgene abundance and reducingnirSgene levels over the course of composting. Together, these data suggest thatB.coagulansX3 supplementation provides an effective means of enhancing nitrogen content in the context of cattle manure composting through the regulation of nitrification and denitrification activity.
Thg1 family 3’-5’ RNA polymerases as tools for targeted RNA synthesis
Authors: Jayasinghe Malithi I. , Patel Krishna J. , Jackman Jane E. 
Published: February 2024

ABSTRACTMembers of the 3’-5’ RNA polymerase family, comprised of tRNAHisguanylyltransferase (Thg1) and Thg1-like proteins (TLPs), catalyze templated synthesis of RNA in the reverse direction to all other known 5’-3’ RNA and DNA polymerases. Discovery of enzymes capable of this reaction raised the possibility of exploiting 3’-5’ polymerases for post-transcriptional incorporation of nucleotides to the 5’-end of nucleic acids without ligation, and instead by templated polymerase addition. To date, studies of these enzymes have focused on nucleotide addition to highly structured RNAs, such as tRNA and other non-coding RNA. Consequently, general principles of RNA substrate recognition and nucleotide preferences that might enable broader application of 3’-5’ polymerases have not been elucidated. Here, we investigated the feasibility of using Thg1 or TLPs for multiple nucleotide incorporation to the 5’-end of a short duplex RNA substrate, using a templating RNA oligonucleotide providedin transto guide 5’-end addition of specific sequences. Using optimized assay conditions, we demonstrated a remarkable capacity of certain TLPs to accommodate short RNA substrate-template duplexes of varying lengths with significantly high affinity, resulting in the ability to incorporate a desired nucleotide sequence of up to 8 bases to 5’-ends of the model RNA substrates in a template-dependent manner. This work has further advanced our goals to develop this atypical enzyme family as a versatile nucleic acid 5’-end labeling tool.
Local Anesthetic Systemic Toxicity in Pregnancy: A Retrospective Cohort Analysis
Authors: de Valle Micah K. , Adkison Michael , Stevenson Cooper , Maredia Ruhi , Murugan Shobana 
Published: February 2024

ABSTRACTIntroductionLocal Anesthetic Systemic Toxicity (LAST) is a rare complication of regional anesthesia. Pregnancy is a risk factor due to gestational physiologic changes. Labor and disorders of pregnancy can mask or delay symptoms of LAST, slowing appropriate intervention. This study examines LAST within a larger cohort and identifies features that help distinguish LAST in pregnancy from nonpregnant patients.MethodsThe TriNetX database was used to compare pregnant and nonpregnant patients with LAST from 2013 to 2023. Cohorts were matched on age, race, obesity status, diabetes, metabolic disorders, local anesthetic type, and cardiovascular, liver, kidney, and respiratory disease. Outcomes included prodromal symptoms of LAST and symptoms of cardiac and central nervous system excitation and depression.ResultsMatching occurred for 276 pregnant and 276 nonpregnant patients. Pregnant cohorts had a significantly higher risk of cardiac depression (RR, 1.96 [95% CI 1.44 - 2.66], p<0.01) and significantly lower risk of cardiac excitation (RR, 0.38 [95% CI 0.22-0.63], p<0.01), prodromal symptoms (RR, 0.17 [95% CI 0.09 - 0.33], p<0.01), central nervous system excitation (RR, 0.44 [95% CI 0.21-0.90], p=0.02), and central nervous system depression (RR, 0.24 [95% CI 0.13-0.48], p<0.01) than nonpregnant cohorts.ConclusionPregnant patients with LAST were more likely to exhibit cardiac depression and less likely to manifest prodromal symptoms, cardiac excitation, and central nervous system excitation and depression than nonpregnant patients. Physiological changes during pregnancy and prompt detection and treatment may explain these differences. These findings highlight the variable nature of LAST and how pregnancy may influence its clinical presentation.Key MessagesLocal anesthetic systemic toxicity, a rare complication of regional anesthesia, has variable manifestations within the cardiovascular and central nervous systems. This study identifies local anesthetic systemic toxicity in a larger cohort relative to previous literature, revealing a distinct presentation in pregnant patients compared to their nonpregnant counterparts. These findings emphasize the diverse nature of local anesthetic systemic toxicity and indicate severe complications in pregnancy. Recognition of subtle clinical manifestations in pregnant patients receiving local anesthesia aids accurate diagnosis and timely intervention in the event of toxicity. The present study provides additional insight into local anesthetic systemic toxicity and sets the stage for further investigations in pregnant patients.
The real-world evidence to the effects of primary psychological healthcare system in diluting risks of suicide ideation in underrepresented children/adolescents: an observational, multi-center, population-based, and longitudinal study
Authors: Li Wei , Liu Xuerong , Zhang Qianyu , Tian Xiaobing , Ren Jidong , Han Xiaodi , Shen Chang , Li Yanyan , Chen Ji , Xia Lei , Zhang Jingxuan , Wu Yi , Gong Jie , Lan Hai , Wu Yan ,   Psychological Health Guard for Children and Adolescents Project of China (CPHG) consortium,   Chinese Open Science Network (COSN) consortium, Feng Zhengzhi , Chen Zhiyi 
Published: February 2024

AbstractBackgroundEstablishing primary psychological healthcare system to prevent suicide was eagerly advocated. However, it remains unclear whether such policy-driven and low-cost healthcare systems could be practical, especially with equal benefits for underrepresented children/adolescents. We aimed to examine the real-world practical effects of primary psychological healthcare system in preventing suicide ideation among children/adolescents, particularly underprivileged ones.Method and FindingsThe study employed an observational, multi-center, population-based and longitudinal design. 19,140 children/adolescents sampled from lower- and middle-income areas of western China (Nanchong) with 1-year followed-ups were included, of which majority to underrepresented underprivileged ones. The primary outcome was the incidence for reporting severe suicide ideation after practicing primary psychological healthcare system at 0.5-year and 1-year follow-ups, as contrasts to baseline. Subgroup analysis was conducted to examine the equal benefits of system for underrepresented children/adolescents. After instigating such system, the risks of reporting suicide ideation for included children/adolescents were found significantly lower compared with the control group at 0.5-year (adjust odds ratios [aOR] 0.28, 95%CI 0.23-0.33; p<0.001) and 1-year follow-ups (aOR 0.28, 95%CI 0.23-0.33; p<0.001). The effects were also observed among underrepresented children/adolescents including “left-behind” children/adolescents, “single-parent” children/adolescents and children/adolescents in especially difficult circumstance (CEDC, all pcorrected < 0.001). The effects in CEDC and “left-behind” children/adolescents were found non-inferior to typical developing cohort (all pcorrected < 0.01).ConclusionsPrimary psychological healthcare system was practically effective in reducing risks of suicide ideation in children/adolescents in 1-year, at least, but not yet with fully equal benefits for all the underprivileged ones.
Molecular regulation ofBna1205ams1required for male fertility and development of a recessive genic male-sterility system inBrassica napus
Authors: Xiao Lijing , Zhang Jinze , Yu Kunjiang , yang Xu , Wang Qian , Jin Hairun , Ouyang Qingjing , Tian Entang 
Published: February 2024

AbstractThrough the comprehensive use of two pollination control systems of cytoplasmic male sterility (CMS) and genic male sterility (GMS), the rapeseed yield was increased by more than 20%. However, more hybrid production systems and detailed mechanisms underlying male sterility are required. Here, we reported a novel two-line hybrid production system of 1205A for GMS and also investigated the underlying mechanism for male sterility. Five co-segregated kompetitive allele specific PCR (KASP) markers were developed and validated, which could be used for transferring the male sterility trait into externalB. napusbreeding lines and developing further two-line hybrid production systems of GMS. Inheritance studies detected one gene locus ofBna1205ams1for regulating the male sterility of 1205A. As a potential candidate gene ofBna1205ams1,BnaC03g27700Dwas fine mapped and narrowed down to a 181.47 kb region on chrC03 and validated by functional analysis. The mutation ofBnaC03g27700Din 1205A resulted in large metabolic fluctuations, most of which were involved in aborted tapetal PCD, which could lead to reduced pollen fertility with abnormal pollen exine. The developed new GMS line of 1205AB provided us with the opportunity to identify a new male sterility gene ofBnaC03g27700DinB. napus. The study ofBnaC03g27700Daims to renew the annotation of the gene and provide new resources for basic research on the genetic control of male sterility.
Predictors of good contraception attitude and practice among female students of television studies in Nigeria: a secondary analysis
Authors: Adeoye Philip Adewale , Adeniji Tolu , Agbo Hadizah Abigail 
Published: February 2024

AbstractBackgroundGlobally contraceptive uptake among young people is increasing; so too is the high unmet need for family planning among this population, especially in sub-Saharan Africa. This study assessed attitude and practice item predictors of contraception among female students of Television studies in Nigeria.MethodsThis is a secondary analysis of a cross-sectional study among 227 female TV undergraduates in Nigeria; selected through a simple random technique by balloting. Data was analyzed using SPSS V.25 and qualitative data were presented as frequencies and proportions. Predictors of good attitude and practice were determined by multivariable logistic regression. P-value<0.05 was adjudged significant.Resultsmost (91%) of respondents are aware of contraception. Most (94.9%) of the respondents have had unplanned pregnancies and only 42.1% had ever used contraception. Predictors of good attitude include good knowledge that female sterilization is one way to avoid pregnancy (aOR: 2.244 [95%CI: 1.170, 4.304]; p=0.015); and opportunity for switching in case of side-effect (aOR: 2.310 [95%CI: 1.166, 4.578]; p=0.016); and using both condoms and pills is very effective (aOR: 1.965 [95%CI: 1.005, 3.840]; p=0.048). The predictor of good contraceptive practice is a poor perception of the adequacy of the current method being used (aOR: 3.236 [95%CI: 1.455, 7.196]; p=0.004).ConclusionThis study shows that most respondents are aware of contraception. However, they show poor attitudes to and practice of contraception. There is a need to consolidate comprehensive sex education in all secondary and tertiary institutions; especially in non-science disciplines to improve the attitude and practice of contraception and ensure reproductive well-being and educational development of the girl-child. There is also the need for community action through community dialogue to improve poor contraceptive attitudes among young women.
The role of tour guide competency in the cultural tour experience: the case of Cappadocia
Authors: Kul Erdim , Dedeoğlu Bekir Bora , Nuray Küçükergin Fulden , De Martino Marcella , Okumus Fevzi 
Published: February 2024

PurposeThis study investigates to what extent the values perceived by tourists throughout cultural tours impact their overall satisfaction levels and behavioral intentions related to the destination. This study further examines the moderating role of tour guide competency in the relationship patterns concerned.Design/methodology/approachEmpirical data were collected via a survey from 420 foreign tourists who visited Cappadocia and participated in guided cultural tours. Partial least squares-structural equation modeling was used for data analysis.FindingsStudy results reveal that the effects of quality, emotional, monetary and social value perceptions of tourists gained through cultural tour experiences on their overall satisfaction levels and the effects of overall satisfaction on recommendation and revisit intention are positive and significant. Furthermore, the moderating role of tour guide competency is significant and positive in the relationships between quality value and satisfaction and between satisfaction and revisit intention.Originality/valueThis study offers a critical analysis of discoveries concerning the pivotal role of tour guide competency within the cultural tour experience.
Parent company personnel embeddedness and stock price crash risk: evidence from Chinese enterprise groups
Authors: Li Yanxi , Meng Delin , Hu YunGe 
Published: February 2024

PurposeThis study aims to investigate the influence of parent company personnel embedding on the stock price crash risk (SPCR) of listed companies, along with the moderating effect of disparate locations between parent and subsidiary companies and other major shareholders.Design/methodology/approachThis research empirically tests hypotheses based on a sample of listed subsidiaries in China during the period between 2006 and 2021.FindingsOur results demonstrate that personnel embeddedness in the parent company significantly alleviates SPCR in subsidiaries. This effect is even more substantial when the parent and subsidiary companies are in different places. However, other major shareholders in the subsidiary company weaken it. Our additional analysis indicates that, relative to executive embeddedness, director embeddedness exerts a stronger effect on the SPCR of the subsidiary. Mechanism examination reveals that the information asymmetry and the level of internal control (IC) within the subsidiary are significant channels through which the personnel embeddedness from the parent company influences the SPCR of the subsidiary.Originality/valueThis study expands the literature on how personnel arrangements in corporate groups within emerging countries influence SPCR. We have extended the traditional concept of interlocking directorates to corporate groups, thereby broadening the understanding of the governance effects of interlocking directors and executives from a group perspective.
Critical pedagogy and disability in participatory research: a review
Authors: May Emma 
Published: February 2024

Purpose
The literature review explores how multidisciplinary approaches based on critical pedagogy and participatory research can provide frameworks for equitable partnerships and genuine participation in educational design and research practices. Additionally, the essay aims to expand understandings of equitable engagement within educational research and design based on principles from critical pedagogy.

Design/methodology/approach
The essay draws from diverse literature in the learning sciences, health informatics, industrial design, disability studies, ethnic studies, rehabilitation science, and to a lesser extent HCI research to understand how critical pedagogy and participatory research methods can provide useful frameworks for disabled peoples' equitable engagement and genuine participation in educational research and design. The literature reviewed in the paper concern topics such as participatory approaches to community development with disabled adults, the implementation of university-initiated community partnerships, participatory research with students and disabled people, and the importance of culturally-responsive research practices. The design literature in this review explores various arenas such as the co-design of assistive technologies with disabled children and adults and the design of curricula for students with and without disabilities. This review focuses on research practices that engender disabled peoples' participation in educational research and design, with focus on developing multidisciplinary frameworks for such research.

Findings
The literature review concludes that participatory research methods and critical pedagogy provide useful frameworks for disabled peoples’ participation in educational design and research practices. Critical pedagogy and participatory design allow for the genuine participation of disabled people in the research process.

Social implications
Emphases on collaboration and collective knowledge-building in social transformation are present in scholarship concerning critical pedagogy, participatory research, and disability studies. However, these connections have been routinely underexplored in the literature. This paper aims to underscore these integral connections as a means to build solidarity between disabled and other marginalized people.

Originality/value
The connections between participatory research methods, critical pedagogy, and disability studies have been previously underexplored. The literature review proposes a combined approach, which has the potential to radically transform multiple realms of research beyond the learning and information sciences.
The impact of relationship marketing practices on companies’ market and financial performance in emerging markets
Authors: Rebiazina Vera , Sharko Elena , Berezka Svetlana 
Published: February 2024

PurposeThe paper aims to reveal the impact of relationship marketing (RM) practices adopted by companies in emerging markets on their market and financial performance (FP) over a long-term, 13-year perspective.Design/methodology/approachThe research design combines primary empirical data from 229 Russian companies, based on the Contemporary Marketing Practices (CMP) survey, and objective FP data from official statistical databases for 2008–2020 to verify the impact of RM practices on market and FP in the long term.FindingsThe research underlines the significant impact of RM practices. It is important to notice that the effect of product development (PD) on marketing performance is mediated by competitor orientation. PD affects market and FP, whose roles vary with the return on assets (ROA).Research limitations/implicationsResearch design supplements the subjective survey data with the objective FP data on the ROA to avoid common method bias.Practical implicationsImplementation of RM practices by Russian companies can increase their effectiveness of performance in the long term.Originality/valueThis research shows the positive impact of RM practices on the FP of Russian firms over the past 13 years.