bluuebunny/crossref_metadata_2025_split huggingface dataset:
An interesting dynamic retrograde His bundle potential during the transventricular‐septal process for left bundle branch pacing in a patient with right bundle branch block: A case report
Authors: Peng Xinhui , Jiang Longfu , Chen Sujun , Feng Haiming 
Published: February 2024

AbstractA 67‐year‐old male presented with symptomatic bradycardia caused by atrial fibrillation and underwent His bundle pacing (HBP) and left bundle branch pacing (LBBP). Electrocardiography (ECG) revealed a complete right bundle branch block (RBBB). John Jiang's connecting cable was used during the transventricular septal process. An interesting dynamic retrograde His bundle potential (RHP) was recorded with uninterrupted lead screws.
Do monetary or nonmonetary incentives promote citizens' use of a government crowdsourcing: A case of the City of Omaha's 311‐type of crowdsourcing platform
Authors: Lee Danbee , Kim Yeonkyung , Lee Jooho 
Published: February 2024

AbstractIncreasing numbers of local governments have adopted crowdsourcing platforms to engage citizens in public service provisions. As citizen engagement plays a critical role in the success of government crowdsourcing, we focus on incentive strategies to facilitate it. While studies have considered monetary incentives the primary motivator traditionally, recent research has examined the potency of nonmonetary strategies that stimulate citizens' territorial or civic motivation. Linking the coproduction framework of incentives to expectancy theories as a theoretical framework, we compare the effects of those incentives with vignette experiments that include Omahahotline—a 311‐type of government crowdsourcing platform run by the City of Omaha. The results show that not just a material incentive but also a solidary incentive increases residents' willingness to participate in the platform effectively. By conducting experiments with Omaha residents, we offer implications for local governments to use effective incentive strategies to engage citizens in the government crowdsourcing platform.
Effects of functional polymorphisms of opioid receptor mu 1 and catechol‐O‐methyltransferase on the neural processing of pain
Authors: Cheong Yongjeon , Lee Seonkyoung , Okazawa Hidehiko , Kosaka Hirotaka , Jung Minyoung 
Published: February 2024

AimPain is reconstructed by brain activities and its subjectivity comes from an interplay of multiple factors. The current study aims to understand the contribution of genetic factors to the neural processing of pain. Focusing on the single‐nucleotide polymorphism (SNP) of opioid receptor mu 1 (OPRM1) A118G (rs1799971) and catechol‐O‐methyltransferase (COMT) val158met (rs4680), we investigated how the two pain genes affect pain processing.MethodWe integrated a genetic approach with functional neuroimaging. We extracted genomic DNA information from saliva samples to genotype the SNP of OPRM1 and COMT. We used a percept‐related model, in which two different levels of perceived pain intensities (“low pain: mildly painful” vs “high pain: severely painful”) were employed as experimental stimuli.ResultsLow pain involves a broader network relative to high pain. The distinct effects of pain genes were observed depending on the perceived pain intensity. The effects of low pain were found in supramarginal gyrus, angular gyrus, and anterior cingulate cortex (ACC) for OPRM1 and in middle temporal gyrus for COMT. For high pain, OPRM1 affected the insula and cerebellum, while COMT affected the middle occipital gyrus and ACC.ConclusionOPRM1 primarily affects sensory and cognitive components of pain processing, while COMT mainly influences emotional aspects of pain processing. The interaction of the two pain genes was associated with neural patterns coding for high pain and neural activation in the ACC in response to pain. The proteins encoded by the OPRM1 and COMT may contribute to the firing of pain‐related neurons in the human ACC, a critical center for subjective pain experience.
Does the risk of basal cell carcinoma increase in patients with psoriasis under treatment?
Authors: Sevdem Gülcan Aliye , Ozlem Kucuk Su , Nazan Yilmaz , Nahide Onsun 
Published: February 2024

AbstractBackgroundPsoriasis is a common, chronic, inflammatory disease. Although it mainly affects the skin, it has been associated with a large number of comorbidities. In addition to comorbidities such as depression and psoriatic arthritis, it is known that there is an increased prevalence of cancer in psoriasis patients. Skin cancers, particularly squamous cell carcinoma, have been associated with psoriasis. However, basal cell carcinoma data are limited.Methods346 psoriasis patients and 306 individuals were selected as the control group. There were no differences between the patient and control groups in terms of age and gender. The mean age of the psoriasis patients was 49.9 ± 15.8 years and the control group was 49.4 ± 13.4 years. Sociodemographic data of the patients were recorded. Pharmacological agents used in the treatment of psoriasis were included in the analysis. Disease severity was assessed by the psoriasis area severity index (PASI). In the physical examination of the patients, biopsies were taken from lesions suspicious for BCC. BCC diagnosis was made by histopathologically.ResultsThe frequency of BCC was higher in psoriasis patients than in the control group (6.6% vs. 2.9%, p < .001). Advanced age (p < .001), smoking (p = .003), and arthritis (p < .001) were associated with BCC in psoriasis patients. However, there was no relationship between PASI and BCC (p = .142). Among the psoriasis treatments, only UV therapy was associated with BCC (p = .038). The frequency of PUVA (p < .001) and number of PUVA session (p = .010) was higher in psoriasis patients with BCC rather than NB‐UVB.ConclusionThe frequency of BCC is increased in psoriasis patients. Psoriasis is associated with an increased risk of BCC, especially when treated with PUVA therapy for a long time.
Expectancy and attention bias to spiders: Dissecting anticipation and allocation processes using ERPs
Authors: Abado Elinor , Aue Tatjana , Pourtois Gilles , Okon‐Singer Hadas 
Published: February 2024

AbstractThe current registered report focused on the temporal dynamics of the relationship between expectancy and attention toward threat, to better understand the mechanisms underlying the prioritization of threat detection over expectancy. In the current event‐related potentials experiment, a‐priori expectancy was manipulated, and attention bias was measured, using a well‐validated paradigm. A visual search array was presented, with one of two targets: spiders (threatening) or birds (neutral). A verbal cue stating the likelihood of encountering a target preceded the array, creating congruent and incongruent trials. Following cue presentation, preparatory processes were examined using the contingent negative variation (CNV) component. Following target presentation, two components were measured: early posterior negativity (EPN) and late positive potential (LPP), reflecting early and late stages of natural selective attention toward emotional stimuli, respectively. Behaviorally, spiders were found faster than birds, and congruency effects emerged for both targets. For the CNV, a non‐significant trend of more negative amplitudes following spider cues emerged. As expected, EPN and LPP amplitudes were larger for spider targets compared to bird targets. Data‐driven, exploratory, topographical analyses revealed different patterns of activation for bird cues compared to spider cues. Furthermore, 400–500 ms post‐target, a congruency effect was revealed only for bird targets. Together, these results demonstrate that while expectancy for spider appearance is evident in differential neural preparation, the actual appearance of spider target overrides this expectancy effect and only in later stages of processing does the cueing effect come again into play.
Targeted Liberalization: China's foreign investment regulation reform and its post‐WTO‐accession export surge
Authors: Liang Yang , Lovely Mary E. , Zhang Hongsheng 
Published: February 2024

AbstractTo gain membership in the World Trade Organization (WTO) in 2001, China substantially modified its regulation of foreign direct investment. These reforms coincided with rapid changes in the composition of capital inflows, as the dominant entry mode shifted from joint venture to wholly foreign owned affiliate. Foreign‐invested enterprises contributed a rising share of China's rapidly growing exports. We investigate how much China's foreign ownership liberalization contributed to these observed trends in foreign investment flows and Chinese exports. Accounting for both the set of activities from which it removed foreign equity caps and those into which it newly encouraged investment, we estimate the impact of China's reforms on firm entry and exports using a difference‐in‐differences estimator. To eliminate bias resulting from heterogeneous and dynamic treatment effects, we also apply novel dynamic difference‐in‐differences estimators. We find that removal of foreign equity caps induced entry of wholly foreign owned firms, while having no significant effect on entry of new joint ventures. Concurrently, the designation of new activities for investment incentives induced foreign entry, particularly in the form of joint ventures. Reduced‐form calculations imply that FDI policy changes explain almost 9% of the increase in exports from foreign‐invested firms over the decade studied. The effect was larger in sectors identified as “high‐tech industries” by the Chinese government, as they contribute most of the estimated policy‐driven export growth from foreign‐invested firms. Thus, China's FDI regulation reform following WTO entry was targeted liberalization: elimination of equity share limits induced new foreign entry, while investment incentives encouraged formation of joint ventures.
Differences in the annotation between facial images and videos for training an artificial intelligence for skin type determination
Authors: Lehner Gabriele Maria , Gockeln Laura , Naber Bettina Marie , Thamm Janis Raphael , Schuh Sandra , Duttler Gabriel , Rottenkolber Anna , Hartmann Dennis , Kramer Frank , Welzel Julia 
Published: February 2024

AbstractBackgroundThe Grand‐AID research project, consisting of GRANDEL‐The Beautyness Company, the dermatology department of Augsburg University Hospital and the Chair of IT Infrastructure for Translational Medical Research at Augsburg University, is currently researching the development of a digital skin consultation tool that uses artificial intelligence (AI) to analyze the user's skin and ultimately perform a personalized skin analysis and a customized skin care routine. Training the AI requires annotation of various skin features on facial images. The central question is whether videos are better suited than static images for assessing dynamic parameters such as wrinkles and elasticity. For this purpose, a pilot study was carried out in which the annotations on images and videos were compared.Materials and MethodsStandardized image sequences as well as a video with facial expressions were taken from 25 healthy volunteers. Four raters with dermatological expertise annotated eight features (wrinkles, redness, shine, pores, pigmentation spots, dark circles, skin sagging, and blemished skin) with a semi‐quantitative and a linear scale in a cross‐over design to evaluate differences between the image modalities and between the raters.ResultsIn the videos, most parameters tended to be assessed with higher scores than in the images, and in some cases significantly. Furthermore, there were significant differences between the raters.ConclusionThe present study shows significant differences between the two evaluation methods using image or video analysis. In addition, the evaluation of the skin analysis depends on subjective criteria. Therefore, when training the AI, we recommend regular training of the annotating individuals and cross‐validation of the annotation.
Metrics to evaluate constitutive model fitness based on DIC experiments
Authors: Peshave Amar , Pierron Fabrice , Lava Pascal , Moens David , Vandepitte Dirk 
Published: February 2024

AbstractFull‐field optical experimental techniques such as Digital Image Correlation (DIC) enable measurement of information‐rich heterogeneous strain states. The aim of Material Testing 2.0 is to capitalise on this and design experimental setups for efficient material characterisation using inverse identification techniques such as the Virtual Fields Method (VFM). In that case, however, a priori knowledge of the constitutive model is required, which sometimes is not a trivial task. This limitation can be overcome by identifying multiple constitutive models using the same DIC strain fields and comparing their performance. In this work, we present a methodology to evaluate model performance and compare different constitutive models based on quantitative metrics. DIC experiments were performed on an S‐shaped high density polyethylene test sample under uniaxial load. The heterogeneous DIC strain fields were used in combination with the VFM to identify parameters of hyperelastic constitutive models with increasing complexity. Two deterministic metrics, the equilibrium gap indicator (EGI) and the reconstructed axial force ratio (RAFR), were defined and used to compare the performance of these constitutive models. The impact of spatial smoothing on DIC strain fields was studied by calculating the EGI and RAFR using a digital twin of the DIC experiments. EGI and RAFR metrics provide complementary information regarding the material behaviour, and both are necessary to make an informed choice. In this case study, hyperelastic constitutive models were found not to capture the material stiffness well in the small strain regime. A linear‐elastic contribution exponentially decaying with the equivalent shear stress was added to the hyperelastic models and the corresponding model parameters identified using the VFM. The elastic‐hyperelastic constitutive models were found to perform better than their purely hyperelastic counterparts.
A study of knowledge and acceptance of kidney xenotransplantation among Chinese kidney transplant recipients and candidates
Authors: Tu Yuhao , Zhao Xiangli , Chen Gang , Zhu Lan 
Published: February 2024

AbstractBackgroundIn recent years, the implementation of the first case of pig‐to‐human heart xenotransplantation and the report of three cases of pig‐to‐brain‐dead human recipient kidney transplantation indicate that xenotransplantation is getting closer to clinical application. In the near future, China may also launch clinical trials of kidney xenotransplantation. Therefore, it is necessary to investigate the level of knowledge and acceptance of xenotransplantation among kidney transplant recipients and candidates in China. This study aims to investigate the level of comprehension and acceptance of kidney xenotransplantation in kidney transplant recipients and explore related factors, providing a reference for promoting the application and clinical trials of xenotransplantation in the near future.MethodsA questionnaire was completed by 211 kidney transplant recipients and 21 candidates. Answers to the questionnaires were self‐administered by the respondents. Scores were compared using nonparametric tests, as well as using Chi‐square test or Fisher's exact test to compare differences in answers.ResultsRespondents demonstrated a high score of 75 (out of 100) on knowledge and acceptance of kidney xenotransplantation. The sector “Knowledge and Attitude” received the overall highest score from respondents (85.0 out of 100), while “Risks and Concerns” received the lowest score (50 out of 100). Interestingly, respondents paid more attention to infection risks but showed less concern about rejection or unknown risks. Furthermore, 191 respondents (82.3%) expected that pig kidney xenografts could function for at least 5 years or more. The scores were statistically significant in terms of age, gender, level of education, level of knowledge on the case of xenotransplantation clinical trial, and willingness to donate organs. cognitionConclusionsThe awareness rates of xenotransplantation are high among kidney transplant recipients and candidates, for which the majority showed a generally favorable attitude towards this procedure. Respondents did not have a comprehensive understanding of the specific knowledge of xenotransplantation and expressed more concern about the risk of infection compared to the risk of rejection and other unknown risks, while also expecting long‐term survival similar to allograft transplantation for pig kidney xenografts.
Effects of periodic breathing on sleep at high altitude: a randomized, placebo‐controlled, crossover study using inspiratory CO2
Authors: Ibrahim Abubaker , Stefani Ambra , Cesari Matteo , Roche Johanna , Gatterer Hannes , Holzknecht Evi , Turner Rachel , Vinetti Giovanni , Furian Michael , Heidbreder Anna , Högl Birgit , Siebenmann Christoph 
Published: February 2024

AbstractHypoxia at high altitude facilitates changes in ventilatory control that can lead to nocturnal periodic breathing (nPB). Here, we introduce a placebo‐controlled approach to prevent nPB by increasing inspiratory CO2 and used it to assess whether nPB contributes to the adverse effects of hypoxia on sleep architecture. In a randomized, single‐blinded, crossover design, 12 men underwent two sojourns (three days/nights each, separated by 4 weeks) in hypobaric hypoxia corresponding to 4000 m altitude, with polysomnography during the first and third night of each sojourn. During all nights, subjects’ heads were encompassed by a canopy retaining exhaled CO2, and CO2 concentration in the canopy (i.e. inspiratory CO2 concentration) was controlled by adjustment of fresh air inflow. Throughout the placebo sojourn inspiratory CO2 was ≤0.2%, whereas throughout the other sojourn it was increased to 1.76% (IQR, 1.07%–2.44%). During the placebo sojourn, total sleep time (TST) with nPB was 54.3% (37.4%–80.8%) and 45.0% (24.5%–56.5%) during the first and the third night, respectively (P = 0.042). Increased inspiratory CO2 reduced TST with nPB by an absolute 38.1% (28.1%–48.1%), the apnoea–hypopnoea index by 58.1/h (40.1–76.1/h), and oxygen desaturation index ≥3% by 56.0/h (38.9.1–73.2/h) (all P < 0.001), whereas it increased the mean arterial oxygen saturation in TST by 2.0% (0.4%–3.5%, P = 0.035). Increased inspiratory CO2 slightly increased the percentage of N3 sleep during the third night (P = 0.045), without other effects on sleep architecture. Increasing inspiratory CO2 effectively prevented hypoxia‐induced nPB without affecting sleep macro‐architecture, indicating that nPB does not explain the sleep deterioration commonly observed at high altitudes.
imageKey points
Periodic breathing is common during sleep at high altitude, and it is unclear how this affects sleep architecture.
We developed a placebo‐controlled approach to prevent nocturnal periodic breathing (nPB) with inspiratory CO2 administration and used it to assess the effects of nPB on sleep in hypobaric hypoxia.
Nocturnal periodic breathing was effectively mitigated by an increased inspiratory CO2 fraction in a blinded manner.
Prevention of nPB did not lead to relevant changes in sleep architecture in hypobaric hypoxia.
We conclude that nPB does not explain the deterioration in sleep architecture commonly observed at high altitude.
A robust, scalable, and cost-efficient approach to whole genome sequencing of RSV directly from clinical samples
Authors: Köndgen Sophie , Oh Djin-Ye , Thürmer Andrea , Sedaghatjoo Somayyeh , Patrono Livia V. , Calvignac-Spencer Sébastien , Biere Barbara , Wolff Thorsten , Dürrwald Ralf , Fuchs Stephan , Reiche Janine 
Published: February 2024

ABSTRACT

Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections causing significant morbidity and mortality among children and the elderly; two RSV vaccines and a monoclonal antibody have recently been approved. Thus, there is an increasing need for a detailed and continuous genomic surveillance of RSV circulating in resource-rich and resource-limited settings worldwide. However, robust, cost-effective methods for whole genome sequencing of RSV from clinical samples that are amenable to high-throughput are still scarce. We developed Next-RSV-SEQ, an experimental and computational pipeline to generate whole genome sequences of historic and current RSV genotypes by in-solution hybridization capture-based next generation sequencing. We optimized this workflow by automating library preparation and pooling libraries prior to enrichment in order to reduce hands-on time and cost, thereby augmenting scalability. Next-RSV-SEQ yielded near-complete to complete genome sequences for 98% of specimens with Cp values ≤31, at median on-target reads >93%, and mean coverage depths between ~1,000 and >5,000, depending on viral load. Whole genomes were successfully recovered from samples with viral loads as low as 230 copies per microliter RNA. We demonstrate that the method can be expanded to other respiratory viruses like parainfluenza virus and human metapneumovirus. Next-RSV-SEQ produces high-quality RSV genomes directly from culture isolates and, more importantly, clinical specimens of all genotypes in circulation. It is cost-efficient, scalable, and can be extended to other respiratory viruses, thereby opening new perspectives for a future effective and broad genomic surveillance of respiratory viruses.

IMPORTANCE
Respiratory syncytial virus (RSV) is a leading cause of severe acute respiratory tract infections in children and the elderly, and its prevention has become an increasing priority. Recently, vaccines and a long-acting monoclonal antibody to protect effectively against severe disease have been approved for the first time. Hence, there is an urgent need for genomic surveillance of RSV at the global scale to monitor virus evolution, especially with an eye toward immune evasion. However, robust, cost-effective methods for RSV whole genome sequencing that are suitable for high-throughput of clinical samples are currently scarce. Therefore, we have developed Next-RSV-SEQ, an experimental and computational pipeline that produces reliably high-quality RSV genomes directly from clinical specimens and isolates.