bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treatment and Rehabilitation for Esophageal Cancer—Striving to Meet Obstacles and Long-term Impacts
Authors: Andreasen Trine Kromann , Boje Ida Rübot , Tolstrup Lærke Kjær , Missel Malene , Larsen Malene Kaas 
Published: April 2024

BackgroundFollowing esophagogastric cancer treatment, patients with esophageal cancer and their relatives struggle with adjusting to a new everyday life as they experience various challenges after treatment requiring rehabilitation. Health professionals must address long-term impacts on patients’ health, everyday life, family functioning, and support needs.ObjectiveThis qualitative descriptive study aimed to explore patients’, relatives’, and health professionals’ experience with long-term impacts and rehabilitation after treatment for esophageal cancer.MethodsA qualitative approach based on a phenomenological-hermeneutical methodology was used. Two methods were selected in the study: focus group interviews with health professionals and individual interviews with patients and relatives. Inductive thematic analysis with inspiration from Braun and Clarke was used for data analysis.ResultsThree main themes emerged from the data analysis: (1) forced into limitations in everyday life, (2) learning and navigating a new everyday life, and (3) rehabilitation accepting new normality.ConclusionPatients and relatives experience persistent long-term impacts after treatment for esophageal cancer, making rehabilitation strenuous and demanding. Relatives take considerable responsibility for the patients’ rehabilitation but feel invisible and unsupported by health professionals. Health professionals acknowledge the severity of long-term impacts but fail to prepare patients and relatives for the impacts and do not see the relatives’ need for support.Implications for PracticeThe study emphasizes the need for a proactive approach in the healthcare system, addressing patients’ and relatives’ physical and psychological vulnerability. Cancer nurses need to prepare and involve patients and relatives in treatment, long-term impacts, and rehabilitation.
Imaging of Neuroendocrine Neoplasms; Principles of Treatment Strategies. What Referring Clinicians Want to Know
Authors: Asmundo Luigi , Ambrosini Valentina , Mojtahed Amirkasra , Fanti Stefano , Ferrone Cristina , Hesami Mina , Sertic Madeleine , Najmi Zahra , Furtado Felipe S. , Dhami Ranjodh S. , Anderson Mark A. , Samir Anthony , Sharma Amita , Campana Davide , Ursprung Stephan , Nikolau Konstantin , Domachevsky Liran , Blake Michael A. , Norris Evan C. , Clark Jeffrey W. , Catalano Onofrio A. 
Published: April 2024

AbstractNeuroendocrine neoplasms (NENs) are a diverse group of tumors that express neuroendocrine markers and primarily affect the lungs and digestive system. The incidence of NENs has increased over time due to advancements in imaging and diagnostic techniques. Effective management of NENs requires a multidisciplinary approach, considering factors such as tumor location, grade, stage, symptoms, and imaging findings. Treatment strategies vary depending on the specific subtype of NEN. In this review, we will focus on treatment strategies and therapies including the information relevant to clinicians in order to undertake optimal management and treatment decisions, the implications of different therapies on imaging, and how to ascertain their possible complications and treatment effects.
Prevalence of Sexual Function and Vibrator Use Among Urogynecology Patients
Authors: Roberts Brittany , Rogers Rebecca , Husk Katherine , Walia Jessmehar , Jacobs Bradley , Wolff Gillian , Deverdis Erin C. 
Published: April 2024

Importance
Urogynecology patients often present with sexual dysfunction; limited information on vibrator utilization to improve sexual function in this population exists.

Objective
The aim of this study was to assess patient knowledge of and receptivity to vibrator use.

Study Design
We conducted a cross-sectional, survey-based cohort study. The survey included patient characteristics, Pelvic Floor Distress Inventory-20 (PFDI-20), Pelvic Organ Prolapse/Urinary Incontinence Sexual Questionnaire Short Form 12, and vibrator use questions. Our primary outcome was vibrator utilization rates comparing younger (<65) and older (≥65 years) urogynecology patients.

Results
Of 213 distributed, 165 (78%) surveys were analyzed. Of these, 104 participants (63%) were aged <65 years and 61 (37%) were ≥65 years. Baseline characteristics were similar between groups (all P's > 0.05). Older patients reported less vibrator utilization than younger patients (30% vs 64%, P ≤ 0.001) and were less likely to be sexually active with a partner (36% vs 62%, P = 0.002) or masturbate (23% vs 51%, P ≤ 0.001). Most patients (76%) thought physicians should discuss vibrators with patients who would like to improve their sexual function with no differences between age groups (71% vs 80%, P = 0.17). Among women receptive to vibrator use, in a multivariable analysis, patients who reported masturbation (odds ratio [OR], 13.8; 95% confidence interval [CI], 2.80–67.71), vibrator use in the past (OR, 24.4; 95% CI, 6.65–89.53), or who believed physicians should discuss vibrators in a clinical setting (OR, 11.66; 95% CI, 2.9–46.81) were more receptive to vibrator use to improve sexual function. Age did not influence receptivity.

Conclusions
Vibrator utilization is greater among younger than older patients. Most urogynecologic patients think health care providers should discuss vibrator use with patients who wish to improve sexual function.
Automated quality control of T1-weighted brain MRI scans for clinical research: methods comparison and design of a quality prediction classifier
Authors: Bhalerao Gaurav , Gillis Grace , Dembele Mohamed , Suri Sana , Ebmeier Klaus , Klein Johannes , Hu Michele , Mackay Clare , Griffanti Ludovica 
Published: April 2024

AbstractIntroductionT1-weighted MRI is widely used in clinical neuroimaging for studying brain structure and its changes, including those related to neurodegenerative diseases, and as anatomical reference for analysing other modalities. Ensuring high-quality T1-weighted scans is vital as image quality affects reliability of outcome measures. However, visual inspection can be subjective and time-consuming, especially with large datasets. The effectiveness of automated quality control (QC) tools for clinical cohorts remains uncertain. In this study, we used T1w scans from elderly participants within ageing and clinical populations to test the accuracy of existing QC tools with respect to visual QC and to establish a new quality prediction framework for clinical research use.MethodsFour datasets acquired from multiple scanners and sites were used (N= 2438, 11 sites, 39 scanner manufacturer models, 3 field strengths – 1.5T, 3T, 2.9T, patients and controls, average age 71 ± 8 years). All structural T1w scans were processed with two standard automated QC pipelines (MRIQC and CAT12). The agreement of the accept-reject ratings was compared between the automated pipelines and with visual QC. We then designed a quality prediction framework that combines the QC measures from the existing automated tools and is trained on clinical datasets. We tested the classifier performance using cross-validation on data from all sites together, also examining the performance across diagnostic groups. We then tested the generalisability of our approach when leaving one site out and explored how well our approach generalises to data from a different scanner manufacturer and/or field strength from those used for training.ResultsOur results show significant agreement between automated QC tools and visual QC (Kappa=0.30 with MRIQC predictions; Kappa=0.28 with CAT12’s rating) when considering the entire dataset, but the agreement was highly variable across datasets. Our proposed robust undersampling boost (RUS) classifier achieved 87.7% balanced accuracy on the test data combined from different sites (with 86.6% and 88.3% balanced accuracy on scans from patients and controls respectively). This classifier was also found to be generalisable on different combinations of training and test datasets (leave-one-site-out = 78.2% average balanced accuracy; exploratory models = 77.7% average balanced accuracy).ConclusionWhile existing QC tools may not be robustly applicable to datasets comprised of older adults who have a higher rate of atrophy, they produce quality metrics that can be leveraged to train a more robust quality control classifiers for ageing and clinical cohorts.
Menstrual hygiene management in two districts of Malawi
Authors: Hinton Rebekah G.K. , Tremblay-Lévesque Laurent-Charles , Kanjaye Modesta , Macleod Christopher J.A. , Troldborg Mads , Kalin Robert M. 
Published: April 2024

1AbstractMenstrual hygiene management (MHM) forms a critical component of ensuring access to adequate and equitable sanitation for all, as outlined in SDG 6.2. Despite its importance, little is known about MHM in Malawi, particularly at a household level. Through a household survey of MHM within 2 districts, we evaluated the type of menstrual absorbents used by people who menstruate. Reusable cloths/rags were the most used menstrual absorbent, used by 79.5% of respondents, whilst disposable absorbents, such as tampons and sanitary pads, were used by 18.6% of respondents. Appropriate MHM also incorporates adequate management of MHM materials, including the washing and drying of reusable menstrual absorbents. We evaluated the cleaning of reusable menstrual absorbents; most respondents (90.1%) reported appropriate washing of menstrual absorbents using soap and water, however only 20.3% reported that menstrual absorbents were dried outside in the sun (as is best practise) with most reporting that reusable menstrual absorbents were dried inside their homes. Our findings highlight the need for improved MHM within Malawi, not only in the access and affordability of appropriate menstrual absorbents but also the promotion of appropriate washing and drying of menstrual absorbents.
Prevalence of Social Anxiety Disorder and its associated factors among Foreign-born Undergraduate Students in Türkiye: A Cross-Sectional Study
Authors: Alnemr Lujain , Salama Abdelaziz H. , Abdelrazek Salma , Alfakeer Hussein , Ali Alkhateeb Mohamed , Torun Perihan 
Published: April 2024

AbstractSocial anxiety disorder (SAD) is prevalent among university students, yet data on its severity among foreign-born international undergraduate students in Türkiye remains limited. This study aims to determine the prevalence of SAD and its associated factors within this population. A cross-sectional study was conducted using a Google Form survey distributed across various universities from September 17, 2023, to February 1, 2024. The survey comprised two sections: sociodemographic information and 17 items of the Social Phobia Inventory (SPIN), which measures the frequency and intensity of social anxiety symptoms. Data analysis included descriptive statistics and inferential analysis, multiple regression, and binomial logistic regression. Out of 506 participants, 455 were included in the study. Results revealed that 39.1% exhibited no or very mild symptoms of SAD, while 23.7% experienced mild symptoms, 21.3% faced moderate symptoms, and 11.6% and 4.2% presented with severe to very severe symptoms, respectively. Factors such as gender (p < 0.0001), previous academic failures (p = 0.013), family history of mental health issues (p = 0.009), exercise frequency (p < 0.0001), and perceptions of relationships with classmates (p < 0.0001) were significantly associated with SAD. Females showed a higher probability of SAD compared to males (OR = 1.976). Individuals engaging in over 90 minutes of exercise per week were less likely to have SAD (OR = 0.383), and occasional smokers had a lower risk of SAD compared to non-smokers (OR = 0.422). Our study uncovered a notably elevated prevalence of Social Anxiety Disorder (SAD) among foreign-born undergraduate students in Türkiye. Factors such as being female, having a family history of mental illnesses, studying in a stressful environment, experiencing academic failure, and engaging in less frequent exercise were associated with noticeable symptoms of SAD. These findings emphasize the urgent need for heightened efforts in recognizing and addressing SAD within this population.
Spatial Coding Dysfunction and Network Instability in the Aging Medial Entorhinal Cortex
Authors: Herber Charlotte S. , Pratt Karishma J.B. , Shea Jeremy M. , Villeda Saul A. , Giocomo Lisa M. 
Published: April 2024

SummaryAcross species, spatial memory declines with age, possibly reflecting altered hippocampal and medial entorhinal cortex (MEC) function. However, the integrity of cellular and network-level spatial coding in aged MEC is unknown. Here, we leveragedin vivoelectrophysiology to assess MEC function in young, middle-aged, and aged mice navigating virtual environments. In aged grid cells, we observed impaired stabilization of context-specific spatial firing, correlated with spatial memory deficits. Additionally, aged grid networks shifted firing patterns often but with poor alignment to context changes. Aged spatial firing was also unstable in an unchanging environment. In these same mice, we identified 458 genes differentially expressed with age in MEC, 61 of which had expression correlated with spatial firing stability. These genes were enriched among interneurons and related to synaptic transmission. Together, these findings identify coordinated transcriptomic, cellular, and network changes in MEC implicated in impaired spatial memory in aging.
Adolescent THC impacts on mPFC dopamine-mediated cognitive processes in male and female rats
Authors: Martinez Maricela X. , Alizo Vera Vanessa , Ruiz Christina M. , Floresco Stan B. , Mahler Stephen V. 
Published: April 2024

AbstractRationaleAdolescent cannabis use is linked to later-life changes in cognition, learning, and memory. Rodent experimental studies suggest Δ9-tetrahydrocannabinol (THC) influences development of circuits underlying these processes, especially in the prefrontal cortex, which matures during adolescence.ObjectiveWe determined how 14 daily THC injections (5mg/kg) during adolescence persistently impacts medial prefrontal cortex (mPFC) dopamine-dependent cognition.MethodsIn adult Long Evans rats treated as adolescents with THC (AdoTHC), we quantify performance on two mPFC dopamine-dependent reward-based tasks—strategy set shifting and probabilistic discounting. We also determined how acute dopamine augmentation with amphetamine (0, 0.25, 0.5 mg/kg), or specific chemogenetic stimulation of ventral tegmental area (VTA) dopamine neurons and their projections to mPFC impacts probabilistic discounting.ResultsAdoTHC sex-dependently impacts acquisition of cue-guided instrumental reward seeking, but has minimal effects on set-shifting or probabilistic discounting in either sex. When we challenged dopamine circuits acutely with amphetamine during probabilistic discounting, we found reduced discounting of improbable reward options, with AdoTHC rats being more sensitive to these effects than controls. In contrast, neither acute chemogenetic stimulation of VTA dopamine neurons nor pathway-specific chemogenetic stimulation of their projection to mPFC impacted probabilistic discounting in control rats, although stimulation of this cortical dopamine projection slightly disrupted choices in AdoTHC rats.ConclusionsThese studies confirm a marked specificity in the cognitive processes impacted by AdoTHC exposure. They also suggest that some persistent AdoTHC effects may alter amphetamine-induced cognitive changes in a manner independent of VTA dopamine neurons or their projections to mPFC.
Acute myeloid leukemia mitochondria hydrolyze ATP to resist chemotherapy
Authors: Hagen James T , Montgomery Mclane M , Aruleba Raphael T , Chrest Brett R , Green Thomas D , Kassai Miki , Zeczycki Tonya N , Schmidt Cameron A , Bhowmick Debajit , Tan Su-Fern , Feith David J , Chalfant Charles E , Loughran Thomas P , Liles Darla , Minden Mark D , Schimmer Aaron D , Cabot Myles C , Mclung Joseph M , Fisher-Wellman Kelsey H 
Published: April 2024

ABSTRACTDespite early optimism, therapeutics targeting oxidative phosphorylation (OxPhos) have faced clinical setbacks, stemming from their inability to distinguish healthy from cancerous mitochondria. Herein, we describe an actionable bioenergetic mechanism unique to cancerous mitochondria inside acute myeloid leukemia (AML) cells. Unlike healthy cells which couple respiration to the synthesis of ATP, AML mitochondria were discovered to support inner membrane polarization by consuming ATP. Because matrix ATP consumption allows cells to survive bioenergetic stress, we hypothesized that AML cells may resist cell death induced by OxPhos damaging chemotherapy by reversing the ATP synthase reaction. In support of this, targeted inhibition of BCL-2 with venetoclax abolished OxPhos flux without impacting mitochondrial membrane potential. In surviving AML cells, sustained polarization of the mitochondrial inner membrane was dependent on matrix ATP consumption. Mitochondrial ATP consumption was further enhanced in AML cells made refractory to venetoclax, consequential to downregulations in both the proton-pumping respiratory complexes, as well as the endogenous F1-ATPase inhibitorATP5IF1. In treatment-naive AML,ATP5IF1knockdown was sufficient to drive venetoclax resistance, whileATP5IF1overexpression impaired F1-ATPase activity and heightened sensitivity to venetoclax. Collectively, our data identify matrix ATP consumption as a cancer-cell intrinsic bioenergetic vulnerability actionable in the context of mitochondrial damaging chemotherapy.
Slow kinesin-dependent microtubular transport facilitates ribbon synapse assembly in developing cochlear inner hair cells
Authors: Voorn Roos Anouk , Sternbach Michael , Jarysta Amandine , Rankovic Vladan , Tarchini Basile , Wolf Fred , Vogl Christian 
Published: April 2024

AbstractSensory synapses are characterized by electron-dense presynaptic specializations, so-called synaptic ribbons. In cochlear inner hair cells (IHCs), ribbons play an essential role as core active zone (AZ) organizers, where they tether synaptic vesicles, cluster calcium channels and facilitate the temporally-precise release of primed vesicles. While a multitude of studies aimed to elucidate the molecular composition and function of IHC ribbon synapses, the developmental formation of these signalling complexes remains largely elusive to date. To address this shortcoming, we performed long-term live-cell imaging of fluorescently-labelled ribbon precursors in young postnatal IHCs to track ribbon precursor motion. We show that ribbon precursors utilize the apico-basal microtubular (MT) cytoskeleton for targeted trafficking to the presynapse, in a process reminiscent of slow axonal transport in neurons. During translocation, precursor volume regulation is achieved by highly dynamic structural plasticity – characterized by regularly-occurring fusion and fission events. Pharmacological MT destabilization negatively impacted on precursor translocation and attenuated structural plasticity, whereas genetic disruption of the anterograde molecular motor Kif1a impaired ribbon volume accumulation during developmental maturation. Combined, our data thus indicate an essential role of the MT cytoskeleton and Kif1a in adequate ribbon synapse formation and structural maintenance.
Molecular Dynamics Trajectory Analysis of Permeation (MDTAP): A tool to analyze permeation events across membrane proteins
Authors: Raghuvamsi Palur Venkata , Sundaresan Sruthi , Rathinavelan Thenmalarchelvi 
Published: April 2024

AbstractBackground and ObjectiveMolecular dynamics (MD) simulations are indispensable and versatile in capturing the time-dependent conformational changes of biomolecules to shed light on the concomitant biological processes. MD is used to provide critical mechanistic insights into the transportation of solvent/solute/drug molecules across protein channels embedded in a membrane bilayer. The huge size and volume of the MD trajectories of a membrane-embedded system provide challenges in the analyses of membrane permeation events. Thus, a software, Molecular Dynamics Trajectory Analysis of Permeation (MDTAP), is presented here to analyze the permeation events across membrane-embedded proteins and nucleic acids automatically.MethodsA software is developed here to automatically detect the permeation events across the channels irrespective of their shape and size and the type of solute molecules from the MD trajectories. MDTAP employs bash scripts to fetch information about the permeation, residence time, and diffusion of the molecules of interest in a Linux/Mac-based environment. The source code of MDTAP is freely available to the public, along with installation and usage information on GitHub (attached as supplementary for the review process and will be made accessible to the public through the following link upon acceptance for publication:https://github.com/MBL-lab/MDTAP).ResultsThe efficiency of MDTAP is demonstrated here by considering the MD trajectories of 2 water-conducting channels as test cases:E. coliouter membrane protein Wzi andE. coliAquaporin Z. The dimensions of the channels and their capacity to accommodate and conduct water, the number of permeating water molecules along with the path traced and time taken to cross the channel is validated.ConclusionIn summary, the graphical representation of the time-dependent behavior of the solute/solvent permeation events corresponding to an MD trajectory in MDTAP allows the user to easily visualize the mechanism of permeation, including the localization of the permeating molecule (if any) and permeating path. Thus, MDTAP immensely reduces the difficult task of manually analyzing solute/solvent permeations from the bulk MD trajectories. Such a simplistic representation of permeation events across the protein transporters helps in the design of drug molecules to treat the associated diseases. Further, MDTAP is also designed to characterize the permeation events across artificial nucleic acid channels, considering their importance in recent times.
Bringing PanglaoDB to 5-star Linked Open Data using Wikidata
Authors: Lubiana Tiago , Cavalcante João Vitor F. 
Published: April 2024

AbstractPanglaoDB is a database of cell-type markers widely used for single-cell RNA sequencing data analysis. However, cell types and genes in the database are encoded by free text, lacking proper identifiers. Wikidata, is a freely editable knowledge graph database useful for integrating biomedical knowledge. We thus reasoned that porting PanglaoDB’s markers to the platform could improve their reusability and overall technical quality (FAIRness).We mapped 188 cell types from PanglaoDB to species-neutral terms on Wikidata and created 376 species-specific terms for cell types inHomo sapiensandMus musculus. These terms were enriched with marker information via thehas marker(P8872) property, totaling over 15.000 cell type X marker associations (w.wiki/9iw6). We explored this new subset of the graph via SPARQL queries, illustrating the discovery potential of structured, integrated knowledge. For example, we found a previously unexplored link between rosehip neurons, clozapine, and schizophrenia via theHRH1marker. Besides the graph-based insights, we took time to describe the details of the reconciliation process, hoping to stimulate more resources for a move to a 5-star linked open data format.