bluuebunny/crossref_metadata_2025_split huggingface dataset:
All-Inside ACL Reconstruction: Short Femoral and Tibial Socket Technique
Authors: Sato Dai , Moulton Samuel G. , Lansdown Drew A. , Benjamin Ma C. 
Published: April 2024

Background: Recently, there has been an increase in interest in all-inside anterior cruciate ligament (ACL) reconstruction (ACLR). Regarding graft maturity, graft contact with the bone tunnel is important. All-inside ACLR with short femoral and tibial sockets might allow to increase the contact between the graft and the socket (ie, no interference screw and contact between the graft and bottom of the socket) and can improve graft maturing. Indications: All-inside ACLR with short sockets technique is indicated in patients with primary ACL rupture or ACL graft re-rupture. Technique Description: The patient was placed in supine position. Following examination under anesthesia, diagnostic arthroscopy was performed to assess for meniscus injuries and chondral defects. The surgical technique required 6 steps: (1) graft preparation (4-strand semitendinosus tendon, graft length was about 45 mm, grafts larger than 8 mm), (2) creating femoral socket (5-10 mm), (3) creating tibial tunnel socket (10-15 mm), (4) passing the graft, (5) tensioning the graft, and (6) graft fixation and skin closure. Patients were partial weightbearing with crutches for 3 weeks and used a hinged knee brace for 6 weeks after surgery. Return to activities was based on functional strength recovery, with a return to running targeted at 4 to 5 months after surgery. Results: All-inside ACLR with short sockets technique provides a better chance of return to preinjury level of activity with accompanied patient satisfaction as compared with anteromedial portal technique at 2 years of follow-up. This technique has favorable functional and clinical outcomes with improvement of graft maturing. Discussion/Conclusion: In conclusion, we propose that ACLR with short tibial and femoral socket technique is simple and safe with favorable clinical outcomes. This is an advantageous ACLR technique that can preserve the gracilis muscle and reduce muscle strength loss of affected limbs. In addition, the all-inside technique is circumferential filling of the socket with the graft, which might increase bone to graft contact compared with interference screws. Further study is needed to show the better clinical outcome and the earlier graft maturation compared with ordinary ACLR. Patient Consent Disclosure Statement: The author(s) attests that consent has been obtained from any patient(s) appearing in this publication. If the individual may be identifiable, the author(s) has included a statement of release or other written form of approval from the patient(s) with this submission for publication.
Immune status and selection of patients for immunotherapy in myeloma: a proposal
Authors: Dhodapkar Madhav V. 
Published: April 2024

Abstract
Newer immune-based approaches based on recruitment and redirection of endogenous and/or synthetic immunity such as chimeric antigen receptor T cells or bispecific antibodies are transforming the clinical management of multiple myeloma (MM). Contributions of the immune system to the antitumor effects of myeloma therapies are also increasingly appreciated. Clinical malignancy in MM originates in the setting of systemic immune alterations that begin early in myelomagenesis and regional changes in immunity affected by spatial contexture. Preexisting and therapy-induced changes in immune cells correlate with outcomes in patients with MM including after immune therapies. Here, we discuss insights from and limitations of available data about immune status and outcomes after immune therapies in patients with MM. Preexisting variation in systemic and/or regional immunity is emerging as a major determinant of the efficacy of current immune therapies as well as vaccines. However, MM is a multifocal malignancy. As with solid tumors, integrating spatial aspects of the tumor and consideration of immune targets with the biology of immune cells may be critical to optimizing the application of immune therapy, including T-cell redirection, in MM. We propose 5 distinct spatial immune types of MM that may provide an initial framework for the optimal application of specific immune therapies in MM: immune depleted, immune permissive, immune excluded, immune suppressed, and immune resistant. Such considerations may also help optimize rational patient selection for emerging immune therapies to improve outcomes.
CD70-specific CAR NK cells expressing IL-15 for the treatment of CD19-negative B-cell malignancy
Authors: Guo Shanshan , Lei Wen , Jin Xueli , Liu Hui , Wang James Q. , Deng Wenhai , Qian Wenbin 
Published: April 2024

Abstract
Chimeric antigen receptor (CAR) natural killer (NK) cells can eliminate tumors not only through the ability of the CAR molecule to recognize antigen-expressed cancer cells but also through NK-cell receptors themselves. This overcomes some of the limitations of CAR T cells, paving the way for CAR NK cells for safer and more effective off-the-shelf cellular therapy. In this study, CD70-specific (a pan-target of lymphoma) fourth-generation CAR with 4-1BB costimulatory domain and interleukin-15 (IL-15) was constructed and transduced into cord blood–derived NK cells by Baboon envelope pseudotyped lentiviral vector. CD70-CAR NK cells displayed superior cytotoxic activity in vitro and in vivo against CD19-negative B-cell lymphoma when compared with nontransduced NK cells and CD19-specific CAR NK cells. Importantly, mice that received 2 doses of CD70-CAR NK cells showed effective eradication of tumors, accompanied by increased concentration of plasma IL-15 and enhanced CAR NK cell proliferation and persistence. Our study suggests that repetitive administration-based CAR NK-cell therapy has clinical advantage compared with a single dose of CAR NK cells for the treatment of B-cell lymphoma.
One-pot Eucalyptus globulus wood fractionation in acid-catalyzed mixtures of water and 1-pentanol: an optimization study
Authors: Fernández-Bautista Marcos , Rivas Sandra , Yáñez Remedios , Alonso José Luís , Parajó Juan Carlos 
Published: April 2024

AbstractSamples of Eucalyptus globulus wood were processed in a reaction media made up of 1-pentanol, water, and diluted acid catalyst. The effects of selected variables (proportion of 1-pentanol in the reaction media, catalyst charge and temperature) on wood solubilization and on the composition of the various phases from treatments (solids containing cellulose, pentanol-rich phase containing depolymerized lignin, and the aqueous phase concentrating the hemicellulose-derived products) were assessed by Response Surface Methodology. Numerical optimization based on the statistical models allowed the identification of the best operational conditions, under which extensive removal of both lignin and hemicelluloses was reached at good selectivity toward cellulose solubilization. The treated solids achieved near stoichiometric conversion upon enzymatic hydrolysis performed under diverse operational conditions, and the lignin structural analysis provided data typical for hardwoods. The experimental results confirmed the potential of 1-pentanol as a solvent for Eucalyptus globulus wood-based biorefineries.
Breathable and wearable graphene/waterborne polyurethane coated regenerated polyethylene terephthalate fabrics for motion sensing and thermal therapy
Authors: Zhang Zhou , Zhang Xuzhen , Huang Wenjian , Zheng Xiong , Ding Bona , Wang Xiuhua 
Published: April 2024

AbstractThe functional utilization of recycled polymers has emerged as a current prominent and timely subject. Flexible wearable devices with high sensitivity to conductivity have garnered significant attention in the fields of human healthcare monitoring and personal heat management. One significant obstacle that needs to be addressed is the simultaneous maintenance of both sensing functionality and durability in composite fabrics. In this paper, a collection of durable, breathable, and flexible smart fabric was produced using the scratch coating method. The fabrics were created by utilizing a regenerated polyethylene terephthalate fabric as a base material, incorporating graphene microsheets (G) as a conductive agent, and applying a waterborne polyurethane layer as a surface protective coating. Furthermore, an investigation was conducted to assess their sensing performance and electrothermal performance. The composite fabric exhibits significant advantages in terms of high conductivity (592 S/m), wide strain range, high sensitivity (Gauge factor = 6.04) and fantabulous dynamic stability (2000 cycles) at a mass ratio of Graphene/WPU loading of 8:2. These sensors were successfully utilized to monitor various degrees of real-time human body movements, ranging from significant deformation bending of elbows to slight deformation swallowing. Furthermore, the sensors also exhibit a significant electric heating effect. Specifically, when a voltage of 10 V is applied, the sensors can reach a steady state temperature of 53.3 °C within a mere 30 s. This discovery holds potential for the development of wearable heaters that can be used for on-demand thermal therapy, functional protective clothing, and medical electric heating wearables.
Haplotype diversity of 17 Y-STR in the Iranian population
Authors: Eskandarion Mohammad Reza , Tabrizi Arash Alipour , Shirkoohi Reza , Raoofian Reza , Naji Masume , Pazhoomand Reza , Salari Hooman , Samadirad Bahram , Sabouri Alireza , Zohour Mostafa Montazer , Namazi Hadi , Farhadi Pegah , Baratieh Zohre , Sayyari Minoo , Dadgarmoghaddam Maliheh , Safdarian Esmat , Nikbakht Afrooz , Golshan Farnaz , Baybordi Fatemeh , Madhaji Elham , ShohodiFar Shadi , Tabasi Mohsen , Mohebbi Ramezan 
Published: April 2024

AbstractThe current study aimed to evaluate Y chromosome haplotypes obtained from 1353 unrelated Iranian males using the AmpFlSTRTM YfilerTM kit; 1353 out of the 1353 identified haplotypes were unique. The haplotype diversity (HD) and discriminating capacity (DC) values were 1.00000 and 0.997, respectively. Analysis of genetic distance was performed using molecular variance (AMOVA) and multidimensional scaling plots (MDS), revealing a statistically significant difference between the study population and previous data reported for other Iranian populations and other neighboring countries. The present findings are likely to be useful for forensic casework analyses and kinship investigations.
DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
Authors: Wang Shuo , Zhao XueLei , Li Chang , Dong Jing , Ma JiaCheng , Long YueHong , Xing ZhaoBin 
Published: April 2024

AbstractPlant growth and development can be significantly impacted by drought stress. Plants will adjust the synthesis and accumulation of secondary metabolites to improve survival in times of water constraint. Simultaneously, drought stress can lead to modifications in the DNA methylation status of plants, and these modifications can directly impact gene expression and product synthesis by changing the DNA methylation status of functional genes involved in secondary metabolite synthesis. However, further research is needed to fully understand the extent to which DNA methylation modifies the content of secondary metabolites to mediate plants’ responses to drought stress, as well as the underlying mechanisms involved. Our study found that in Eleutherococcus senticosus (E. senticosus), moderate water deprivation significantly decreased DNA methylation levels throughout the genome and at the promoters of EsFPS, EsSS, and EsSE. Transcription factors like EsMYB-r1, previously inhibited by DNA methylation, can re-bind to the EsFPS promotor region following DNA demethylation. This process promotes gene expression and, ultimately, saponin synthesis and accumulation. The increased saponin levels in E. senticosus acted as antioxidants, enhancing the plant’s adaptability to drought stress.
Assessment of the changes in growth, photosynthetic traits and gene expression in Cynodon dactylon against drought stress
Authors: Noor Maryam , Fan Jibiao , Kaleem Muhammad , Akhtar Muhammad Tanveer , Jin Shixuan , Nazir Usman , Zhang Chuan-Jie , Yan Xuebing 
Published: April 2024

AbstractDrought stress considered a key restrictive factor for a warm-season bermudagrass growth during summers in China. Genotypic variation against drought stress exists among bermudagrass (Cynodon sp.), but the selection of highly drought-tolerant germplasm is important for its growth in limited water regions and for future breeding. Our study aimed to investigate the most tolerant bermudagrass germplasm among thirteen, along latitude and longitudinal gradient under a well-watered and drought stress condition. Current study included high drought-resistant germplasm, “Tianshui” and “Linxiang”, and drought-sensitive cultivars; “Zhengzhou” and “Cixian” under drought treatments along longitude and latitudinal gradients, respectively. Under water deficit conditions, the tolerant genotypes showed over-expression of a dehydrin gene cdDHN4, antioxidant genes Cu/ZnSOD and APX which leads to higher antioxidant activities to scavenge the excessive reactive oxygen species and minimizing the membrane damage. It helps in maintenance of cell membrane permeability and osmotic adjustment by producing organic osmolytes. Proline an osmolyte has the ability to keep osmotic water potential and water use efficiency high via stomatal conductance and maintain transpiration rate. It leads to optimum CO2 assimilation rate, high chlorophyll contents for photosynthesis and elongation of leaf mesophyll, palisade and thick spongy cells. Consequently, it results in elongation of leaf length, stolon and internode length; plant height and deep rooting system. The CdDHN4 gene highly expressed in “Tianshui” and “Youxian”, Cu/ZnSOD gene in “Tianshui” and “Linxiang” and APX gene in “Shanxian” and “Linxiang”. The genotypes “Zhongshan” and “Xiaochang” showed no gene expression under water deficit conditions. Our results indicate that turfgrass show morphological modifications firstly when subjected to drought stress; however the gene expression is directly associated and crucial for drought tolerance in bermudagrass. Hence, current research has provided excellent germplasm of drought tolerant bermudagrass for physiological and molecular study and future breeding.
Mitigating drought stress in wheat plants (Triticum Aestivum L.) through grain priming in aqueous extract of spirulina platensis
Authors: Elnajar Mustafa , Aldesuquy Heshmat , Abdelmoteleb Mohamed , Eltanahy Eladl 
Published: April 2024

Abstract
Background
The study focuses on the global challenge of drought stress, which significantly impedes wheat production, a cornerstone of global food security. Drought stress disrupts cellular and physiological processes in wheat, leading to substantial yield losses, especially in arid and semi-arid regions. The research investigates the use of Spirulina platensis aqueous extract (SPAE) as a biostimulant to enhance the drought resistance of two Egyptian wheat cultivars, Sakha 95 (drought-tolerant) and Shandawel 1 (drought-sensitive). Each cultivar’s grains were divided into four treatments: Cont, DS, SPAE-Cont, and SPAE + DS. Cont and DS grains were presoaked in distilled water for 18 h while SPAE-Cont and SPAE + DS were presoaked in 10% SPAE, and then all treatments were cultivated for 96 days in a semi-field experiment. During the heading stage (45 days: 66 days), two drought treatments, DS and SPAE + DS, were not irrigated. In contrast, the Cont and SPAE-Cont treatments were irrigated during the entire experiment period. At the end of the heading stage, agronomy, pigment fractions, gas exchange, and carbohydrate content parameters of the flag leaf were assessed. Also, at the harvest stage, yield attributes and biochemical aspects of yielded grains (total carbohydrates and proteins) were evaluated.

Results
The study demonstrated that SPAE treatments significantly enhanced the growth vigor, photosynthetic rate, and yield components of both wheat cultivars under standard and drought conditions. Specifically, SPAE treatments increased photosynthetic rate by up to 53.4%, number of spikes by 76.5%, and economic yield by 190% for the control and 153% for the drought-stressed cultivars pre-soaked in SPAE. Leaf agronomy, pigment fractions, gas exchange parameters, and carbohydrate content were positively influenced by SPAE treatments, suggesting their effectiveness in mitigating drought adverse effects, and improving wheat crop performance.

Conclusion
The application of S. platensis aqueous extract appears to ameliorate the adverse effects of drought stress on wheat, enhancing the growth vigor, metabolism, and productivity of the cultivars studied. This indicates the potential of SPAE as an eco-friendly biostimulant for improving crop resilience, nutrition, and yield under various environmental challenges, thus contributing to global food security.
The automatic activity of abdominal muscles during stable and unstable standing postural tasks in older adults with and without low back pain- A cross-sectional study
Authors: Kalantari Mohammad , ShahAli Shabnam , Dadgoo Mehdi , Tabatabaei Abbas 
Published: April 2024

Abstract
Background
The postural control and abdominal muscles’ automatic activity were found to be impaired in subjects with low back pain (LBP) during static activities. However, the studies are predominantly conducted on younger adults and a limited number of studies have evaluated abdominal muscles’ automatic activity during dynamic standing activities in subjects with LBP. The present study investigated the automatic activity of abdominal muscles during stable and unstable standing postural tasks in older adults with and without LBP.

Methods
Twenty subjects with and 20 subjects without LBP were included. The thickness of the transversus abdominis (TrA), internal oblique (IO), and external oblique (EO) muscles was measured during rest (in supine), static, and dynamic standing postural tasks. To estimate automatic muscle activity, each muscle’s thickness during a standing task was normalized to its thickness during the rest. Standing postural tasks were performed using the Biodex Balance System.

Results
The mixed-model analysis of variance revealed that task dynamicity significantly affected thickness change only in the TrA muscle (P = 0.02), but the main effect for the group and the interaction were not significantly different (P > 0.05). There were no significant main effects of the group, task dynamicity, or their interaction for the IO and EO muscles (P > 0.05). During dynamic standing, only the TrA muscle in the control group showed greater thickness changes than during the static standing task (P < 0.05).

Conclusions
Standing on a dynamic level increased the automatic activity of the TrA muscle in participants without LBP compared to standing on a static level. Further research is required to investigate the effects of TrA muscle training during standing on dynamic surfaces for the treatment of older adults with LBP.
Immunization with a peptide mimicking lipoteichoic acid induces memory B cells in BALB/c mice
Authors: Yi Xia-Yu , Hou Xiao-Rui , Huang Zhao-Xia , Zhu Ping , Liu Bei-Yi 
Published: April 2024

Abstract
Background
There is an urgent clinical need for developing novel immunoprophylaxis and immunotherapy strategies against Staphylococcus aureus (S. aureus). In our previous work, immunization with a tetra-branched multiple antigenic peptide, named MAP2-3 that mimics lipoteichoic acid, a cell wall component of S. aureus, successfully induced a humoral immune response and protected BALB/c mice against S. aureus systemic infection. In this study, we further investigated whether vaccination with MAP2-3 can elicit immunologic memory.

Methods
BALB/c mice were immunized with MAP2-3 five times. After one month of the last vaccination, mice were challenged with heat-killed S. aureus via intraperitoneal injection. After a 7-day inoculation, the percentage of plasma cells, memory B cells, effector memory T cells, and follicular helper T cells were detected by flow cytometry. The levels of IL-6, IL-21, IL-2, and IFN-γ were measured by real-time PCR and ELISA. Flow cytometry results were compared by using one-way ANOVA or Mann-Whitney test, real-time PCR results were compared by using one-way ANOVA, and ELISA results were compared by using one-way ANOVA or student’s t-test.

Results
The percentage of plasma cells and memory B cells in the spleen and bone marrow from the MAP2-3 immunized mice was significantly higher than that from the control mice. The percentage of effector memory T cells in spleens and lymphoid nodes as well as follicular helper T cells in spleens from the MAP2-3 immunized mice were also higher. Moreover, the levels of IL-6 and IL-21, two critical cytokines for the development of memory B cells, were significantly higher in the isolated splenocytes from immunized mice after lipoteichoic acid stimulation.

Conclusions
Immunization with MAP2-3 can efficiently induce memory B cells and memory T cells.
Detection of circulating tumor DNA in plasma of patients with primary CNS lymphoma by digital droplet PCR
Authors: Zhong Yujie , Tan Geok Wee , Bult Johanna , Veltmaat Nick , Plattel Wouter , Kluiver Joost , Enting Roelien , Diepstra Arjan , van den Berg Anke , Nijland Marcel 
Published: April 2024

Abstract
Background
Primary central nervous system lymphoma (PCNSL) are rare mature B-cell lymphoproliferative diseases characterized by a high incidence of MYD88 L265P and CD79B Y196 hotspot mutations. Diagnosis of PCNSL can be challenging. The aim of the study was to analyze the detection rate of the MYD88 L265P and CD79B Y196 mutation in cell free DNA (cfDNA) in plasma of patients with PCNSL.

Methods
We analyzed by digital droplet PCR (ddPCR) to determine presence of the MYD88 L265P and CD79B Y196 hotspot mutations in cfDNA isolated from plasma of 24 PCNSL patients with active disease. Corresponding tumor samples were available for 14 cases. Based on the false positive rate observed in 8 healthy control samples, a stringent cut-off for the MYD88 L265P and CD79B Y196 mutation were set at 0.3% and 0.5%, respectively.

Results
MYD88 L265P and CD79B Y196 mutations were detected in 9/14 (64%) and 2/13 (15%) tumor biopsies, respectively. In cfDNA samples, the MYD88 L265P mutation was detected in 3/24 (12.5%), while the CD79B Y196 mutation was not detected in any of the 23 tested cfDNA samples. Overall, MYD88 L265P and/or CD79B Y196 were detected in cfDNA in 3/24 cases (12.5%). The detection rate of the combined analysis did not improve the single detection rate for either MYD88 L265P or CD79B Y196.

Conclusion
The low detection rate of MYD88 L265P and CD79B Y196 mutations in cfDNA in the plasma of PCNSL patients argues against its use in routine diagnostics. However, detection of MYD88 L265P by ddPCR in cfDNA in the plasma could be considered in challenging cases.