bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tribo-Erosion Analysis of HVOF Colmonoy-88-Coated Pump Impeller Steel in Coal Ash Particle Slurries
Authors: Singh Gurmeet , Jain Pulkit , Gill Harjot Singh 
Published: March 2024

Abstract
Coal ash slurries hold paramount importance in the service life of the coal and mining industries, significantly impacting their operational efficiency and durability. The literature reveals that the prominent dominating parameters such as rotational speed, concentration, time duration, and particle shape and size have a major impact on erosion wear. This research aims to explore the effects of coal ash slurries on Colmonoy-88-coated pump impeller steel, employing a combination of design of experiments (DOE) and digital image analysis (DIA). Through slurry experiments, it is observed that erosion wear increases non-linearly with an increase in influencing parameters. The particle size emerges as the most significant factor, followed by concentration, speed, and time. Additionally, DIA is leveraged to validate the influence of various erodent particle sizes on the erosion wear of the pump impeller steel. Results indicate that coal particles possess irregular shapes and sizes compared to fly ash and bottom ash, consequently resulting in larger erosion wear due to their lowest circularity factor (0.72). Furthermore, smaller particles exhibit lower erosion wear-rates compared to larger ones. This study sheds light on the intricate dynamics of erosion wear in the context of coal ash slurries, offering insights crucial for optimizing pump impeller steel durability in similar operational environments.
Investigating the association between nitrate dosing and nitrite generation by the human oral microbiota in continuous culture
Authors: Willmott Thomas , Serrage Hannah J. , Cottrell Elizabeth C. , Humphreys Gavin J. , Myers Jenny , Campbell Paul M. , McBain Andrew J. 
Published: March 2024

ABSTRACT

The generation of nitrite by the oral microbiota is believed to contribute to healthy cardiovascular function, with oral nitrate reduction to nitrite associated with systemic blood pressure regulation. There is the potential to manipulate the composition or activities of the oral microbiota to a higher nitrate-reducing state through nitrate supplementation. The current study examined microbial community composition and enzymatic responses to nitrate supplementation in sessile oral microbiota grown in continuous culture. Nitrate reductase (NaR) activity and nitrite concentrations were not significantly different to tongue-derived inocula in model biofilms. These were generally dominated by
Streptococcus
spp., initially, and a single nitrate supplementation resulted in the increased relative abundance of the nitrate-reducing genera
Veillonella
,
Neisseria
, and
Proteus
spp. Nitrite concentrations increased concomitantly and continued to increase throughout oral microbiota development. Continuous nitrate supplementation, over a 7-day period, was similarly associated with an elevated abundance of nitrate-reducing taxa and increased nitrite concentration in the perfusate. In experiments in which the models were established in continuous low or high nitrate environments, there was an initial elevation in nitrate reductase, and nitrite concentrations reached a relatively constant concentration over time similar to the acute nitrate challenge with a similar expansion of
Veillonella
and
Neisseria
. In summary, we have investigated nitrate metabolism in continuous culture oral biofilms, showing that nitrate addition increases nitrate reductase activity and nitrite concentrations in oral microbiota with the expansion of putatively NaR-producing taxa.

IMPORTANCE
Clinical evidence suggests that blood pressure regulation can be promoted by nitrite generated through the reduction of supplemental dietary nitrate by the oral microbiota. We have utilized oral microbiota models to investigate the mechanisms responsible, demonstrating that nitrate addition increases nitrate reductase activity and nitrite concentrations in oral microbiota with the expansion of nitrate-reducing taxa.
A human milk oligosaccharide prevents intestinal inflammation in adulthood via modulating gut microbial metabolism
Authors: Schalich Kasey M. , Buendia Matthew A. , Kaur Harpreet , Choksi Yash A. , Washington M. Kay , Codreanu Gabriela S. , Sherrod Stacy D. , McLean John A. , Peek, Jr. Richard M. , Acra Sari A. , Townsend Steven D. , Yan Fang 
Published: March 2024

ABSTRACT

Observational evidence suggests that human milk oligosaccharides (HMOs) promote the growth of commensal bacteria in early life and adulthood. However, the mechanisms by which HMOs benefit health through modulation of gut microbial homeostasis remain largely unknown. 2′-fucosyllactose (2′-FL) is the most abundant oligosaccharide in human milk and contributes to the essential health benefits associated with human milk consumption. Here, we investigated how 2′-FL prevents colitis in adulthood through its effects on the gut microbial community. We found that the gut microbiota from adult mice that consumed 2′-FL exhibited an increase in abundance of several health-associated genera, including
Bifidobacterium
and
Lactobacillus
. The 2′-FL-modulated gut microbial community exerted preventive effects on colitis in adult mice. By using
Bifidobacterium infantis
as a 2′-FL-consuming bacterial model, exploratory metabolomics revealed novel 2′-FL-enriched secretory metabolites by
Bifidobacterium infantis
, including pantothenol. Importantly, pantothenate significantly protected the intestinal barrier against oxidative stress and mitigated colitis in adult mice. Furthermore, microbial metabolic pathway analysis identified 26 dysregulated metabolic pathways in fecal microbiota from patients with ulcerative colitis, which were significantly regulated by 2′-FL treatment in adult mice, indicating that 2′-FL has the potential to rectify dysregulated microbial metabolism in colitis. These findings support the contribution of the 2′-FL-shaped gut microbial community and bacterial metabolite production to the protection of intestinal integrity and prevention of intestinal inflammation in adulthood.

IMPORTANCE

At present, neither basic research nor clinical studies have revealed the exact biological functions or mechanisms of action of individual oligosaccharides during development or in adulthood. Thus, it remains largely unknown whether human milk oligosaccharides could serve as effective therapeutics for gastrointestinal-related diseases. Results from the present study uncover 2′-FL-driven alterations in bacterial metabolism and identify novel
B. infantis
-secreted metabolites following the consumption of 2′-FL, including pantothenol. This work further demonstrates a previously unrecognized role of pantothenate in significantly protecting the intestinal barrier against oxidative stress and mitigating colitis in adult mice. Remarkably, 2′-FL-enhanced bacterial metabolic pathways are found to be dysregulated in the fecal microbiota of ulcerative colitis patients. These novel metabolic pathways underlying the bioactivities of 2′-FL may lay a foundation for applying individual oligosaccharides for prophylactic intervention for diseases associated with impaired intestinal homeostasis.
The draft genome sequence of
bla
CTX-M-270
-carrying
Kluyvera ascorbata
isolated from human sputum
Authors: Liu Lina , Feng Yu , Wen Hongxia , Zong Zhiyong 
Published: March 2024

ABSTRACT

Kluyvera ascorbata
is recognized as an opportunistic pathogen of the
Enterobacteriaceae
family but remains less studied. We report the draft genome of a
K. ascorbata
clinical strain recovered from human sputum, comprising approximately 5.18 million bases and harboring an intrinsic gene encoding the extended-spectrum β-lactamase CTX-M-270.
The impact of
Lactococcus lactis
KUST48 on the transcription profile of
Aeromonas hydrophila
-infected zebrafish spleen
Authors: Chen Jiayu , Li Qiuyue , Fan Lili , Xie Liqin , Zhang Qilin , Deng Xianyu 
Published: March 2024

ABSTRACT

Aeromonas hydrophila
, an aquatic pathogenic bacterium, has been found to infect many fish species and cause huge aquaculture losses. Antibiotics are the most common drugs used to treat these infections. However, antibiotic abuse can lead to the development of antibiotic resistance. Probiotics have the potential to replace antibiotics for preventing infections. Zebrafish (
Danio rerio
) is a model organism used to study the innate immune system and host-pathogen interactions. Currently, there is little information on how the fish immune system responds to
A. hydrophila
and probiotic treatment. To increase the understanding of the molecular mechanisms behind the zebrafish defense against
A. hydrophila
and provide evidence that antibiotics can be replaced by probiotics, a transcriptome analysis of the zebrafish spleen was conducted 48 hours after infection by
A. hydrophila
, as well as after treatment using
Lactococcus lactis
KUST48 4 hours after infection. A total of 36,499 genes were obtained. There were 3,337 genes found to have significant differential expression between treatment and control groups. According to further annotation and enrichment analysis, differentially expressed genes (DEGs) were involved in signal transduction, endocrine system cancer, and the immune system. Insulin resistance disappeared in the zebrafish after treatment. Quantitative real-time PCR was performed to confirm the significant regulation of immune defense DEGs, the results of which were consistent with the RNA-sequencing data. These results could serve as a basis for future studies on the immune response to
A. hydrophila
and provide suggestions for probiotic alternatives to antibiotics, which will be of great significance to aquaculture and environmental protection.

IMPORTANCE

In recent years, the unreasonable use of antibiotics has led to the emergence of drug-resistant pathogenic bacteria, antibiotic residues, cross infection, toxic side effects, and so on, which has caused a serious threat to human food safety and life health. In recent years, many studies have demonstrated the potential of probiotics as a substitute for antibiotics, but there is still a lack of understanding of the molecular mechanisms underlying probiotic therapy. We conduct a research on the impact of
Lactococcus lactis
KUST48 on the transcription profile of
Aeromonas hydrophila
-infected zebrafish spleen. Mortality of zebrafish infected with
A. hydrophila
was significantly reduced after treatment with
L. lactis
KUST48. Our results can help to strengthen our understanding of the pathogenic mechanisms of zebrafish and provide a valuable reference for the molecular mechanisms of probiotic therapy.
Cold Atom Gravimeter Based on an Atomic Fountain and a Microwave Transition
Authors: Afanasiev A. E. , Skakunenko P. I. , Balykin V. I. 
Published: March 2024

A method based on measuring the shift of Ramsey spectral line in an atomic fountain in the gravitational field has been proposed to develop an atomic gravimeter involving the atomic fountain on ultracold atoms. The accuracy of the measurement of the gravitational acceleration with a fountain microwave frequency standard on Cs atoms is $$\delta g = 2 \times {{10}^{{ - 6}}}g{\text{/}}\sqrt {{{\tau }_{{\text{a}}}}} $$. The achievable accuracy at the integration time τa = 10 000 s is $$\delta g \approx 2 \times {{10}^{{ - 8}}}g \approx 20{\kern 1pt} $$ μGal.
Wunderlich syndrome in a patient with pyelonephritis
Authors: Chia Joshua Chin Howe , Chong Chiara Jiamin , Yong Yan Rong , Tan Poh Yong 
Published: March 2024

An elderly female patient with left pyelonephritis developed worsening left flank pain, hypotension and a drop in haemoglobin (Hb) from 97 g/L to 67g/L on the third day of her admission. There was no recent trauma, history of coagulopathy or risk factors for renal malignancy or vascular disease.A contrasted CT scan of the kidneys revealed a 3.8 cm left renal subcapsular haematoma with no active contrast extravasation. Her atraumatic subcapsular haematoma fulfils two out of three clinical features of Lenk’s triad (acute flank pain, hypovolaemic shock), suggestive of Wunderlich syndrome. Urine and blood cultures grewKlebsiella pneumoniaeand she was managed conservatively with culture-directed antibiotics, fluids and blood products.Wunderlich syndrome is a rare complication of pyelonephritis and should be considered in patients with pyelonephritis who develop acute severe flank pain, Hb drop and haemodynamic instability. Appropriate medical and surgical therapies need to be instituted early to ensure good outcomes.
Soil thermal properties: influence of no-till cover crops
Authors: Haque Md Ariful , Ku Seockmo , Haruna Samuel I. 
Published: March 2024

Soil thermal properties, which determine heat transport, can influence soil health parameters and crop productivity. The objective of this study was to evaluate the 2-year effects of no-till cover crops (CCs) and no-till no cover crop (NC) on soil thermal properties (thermal conductivity (λ), volumetric heat capacity ( CV), and thermal diffusivity ( D)). Two levels of CCs were used for this study: CC versus NC. The CCs included crimson clover ( Trifolium incarnatum L.), hairy vetch ( Vicia villosa Roth.), winter peas ( Lathyrus hirsutus L.), oats ( Avena sativa), winter wheat ( Triticum aestivum L.), triticale ( Triticale hexaploide Lart.), flax ( Linum usitassimum L.), and barley ( Hordeum vulgare L.). Soil samples were collected at 0–10, 10–20, and 20–30 cm depths and their λ, CV, and D were measured in the laboratory. Additionally, soil organic carbon, bulk density (BD), and volumetric water content (ϴ) at saturation, −33 kPa, and −100 kPa soil water pressures were measured. Results showed that BD was 18% and 14% higher under CC compared with NC management during 2021 and 2022, respectively. Furthermore, ϴ at all measured soil water pressures was slightly higher under CC compared with NC management during both years. As a result, λ and D were significantly higher under NC compared with CC management, while CV was significantly higher under CC compared with NC management, during both years and at all measured soil water pressures. Generally, soil thermal properties were directly proportional to ϴ, suggesting that ϴ may be the most important factor influencing soil thermal properties.
FDA Approval Summary: Enfortumab Vedotin plus Pembrolizumab for Cisplatin-Ineligible Locally Advanced or Metastatic Urothelial Carcinoma
Authors: Maguire William F. , Lee Daniel , Weinstock Chana , Gao Xin , Bulik Catharine C. , Agrawal Sundeep , Chang Elaine , Hamed Salaheldin S. , Bloomquist Erik W. , Tang Shenghui , Pazdur Richard , Kluetz Paul G. , Amiri-Kordestani Laleh , Suzman Daniel L. 
Published: March 2024

Abstract
On April 3, 2023, the FDA granted accelerated approval to enfortumab vedotin-ejfv (EV) plus pembrolizumab for treatment of patients with locally advanced or metastatic urothelial carcinoma who are ineligible for cisplatin-containing chemotherapy. Substantial evidence of effectiveness was obtained from EV-103/KEYNOTE-869 (NCT03288545), a multicohort study. Across cohorts, a total of 121 patients received EV 1.25 mg/kg (maximum of 125 mg) intravenously on days 1 and 8 of a 21-day cycle plus pembrolizumab 200 mg intravenously on day 1 of each 21-day cycle until disease progression or unacceptable toxicity. The major efficacy outcome measures were objective response rate (ORR) and duration of response (DoR), determined by blinded independent central review using RECIST v1.1. The confirmed ORR in 121 patients was 68% (95% confidence interval, 59–76), including 12% with complete responses. The median DoR for the 82 responders was 22 months (range: 1+ to 46+). The safety profile of the combination comprised adverse reactions expected to occur with the corresponding monotherapies, but with overall increased frequency of adverse reactions, including skin toxicity, pneumonitis, and peripheral neuropathy. The article summarizes the data and the FDA thought process supporting accelerated approval of EV + pembrolizumab, as well as additional exploratory analyses conducted by the FDA.
Acquired Multidrug Resistance in AML Is Caused by Low Apoptotic Priming in Relapsed Myeloblasts
Authors: Olesinski Elyse A. , Bhatia Karanpreet Singh , Wang Chuqi , Pioso Marissa S. , Lin Xiao Xian , Mamdouh Ahmed M. , Ng Shu Xuan , Sandhu Vedant , Jasdanwala Shaista Shabbir , Yilma Binyam , Bohl Stephan , Ryan Jeremy A. , Malani Disha , Luskin Marlise R. , Kallioniemi Olli , Porkka Kimmo , Adamia Sophia , Chng Wee Joo , Osato Motomi , Weinstock David M. , Garcia Jacqueline S. , Letai Anthony , Bhatt Shruti 
Published: March 2024

Abstract

In many cancers, mortality is associated with the emergence of relapse with multidrug resistance (MDR). Thus far, the investigation of cancer relapse mechanisms has largely focused on acquired genetic mutations. Using acute myeloid leukemia (AML) patient-derived xenografts (PDX), we systematically elucidated a basis of MDR and identified drug sensitivity in relapsed AML. We derived pharmacologic sensitivity for 22 AML PDX models using dynamic BH3 profiling (DBP), together with genomics and transcriptomics. Using in vivo acquired resistant PDXs, we found that resistance to unrelated, narrowly targeted agents in distinct PDXs was accompanied by broad resistance to drugs with disparate mechanisms. Moreover, baseline mitochondrial apoptotic priming was consistently reduced regardless of the class of drug-inducing selection. By applying DBP, we identified drugs showing effective in vivo activity in resistant models. This study implies evasion of apoptosis drives drug resistance and demonstrates the feasibility of the DBP approach to identify active drugs for patients with relapsed AML.

Significance:
Acquired resistance to targeted therapy remains challenging in AML. We found that reduction in mitochondrial priming and common transcriptomic signatures was a conserved mechanism of acquired resistance across different drug classes in vivo. Drugs active in vivo can be identified even in the multidrug resistant state by DBP.
Abstract 004: Summary of MT2020+ Research Internship Progress from 2021 to 2023
Authors: Saigal Khushi , Saxena Tanya , Mairal Anurag , Yavagal Dileep R 
Published: March 2024

Introduction
Mission Thrombectomy, established in 2016 by the Society of Vascular and Interventional Neurology, aims to enhance global accessibility to mechanical thrombectomy surgery for strokes caused by large vessel occlusion in the brain. High school students interested in exposure to global health have limited access to get exposure in this rapidly expanding area of public health. The MT2020+ student internship was designed in collaboration with MT2020+ to provide such an experience to US High School students. Students designed and carried out projects to raise stroke awareness among other high school students and communities while studying the barriers to stroke treatment. We report on this novel internship program's methodology, results, and outcomes.

Methods
The MT2020+ internship program followed a comprehensive framework. Three phases were implemented: Phase A involved secondary research, stakeholder identification, and the formation of a detailed survey and interview questions. Stakeholders included neurologists, neuro‐interventionalists, neuro‐radiologists, and members of stroke societies. Countries in the region of interest were categorized into three groups based on stroke and COVID‐19 data. A survey template was created in Phase B, and stakeholders were contacted for data collection. Interviews were conducted to delve deeper into survey responses. Phase C focused on analyzing the collected data, articulating the key barriers, and generating a written report. Interns also developed interventions to address key barriers identified during the research.

Results
The first cohort (2020) comprised 8 interns who investigated barriers to stroke treatment across different regions. The interns received survey responses and conducted interviews with stakeholders. Their research covered the U.S. & North America, Latin America, Europe, Africa, Asia, and Australia. The outcomes varied, with different levels of engagement. One student developed an app in Peru to address transportation, educational, and rehabilitation barriers. The second cohort (2021) had 2 interns who focused on Latin America and Asia, working on rehabilitation apps. The third cohort (2022) included 4 interns studying Asia, Australia/New Zealand, North America/US, and Latin America. Their interventions ranged from creating stroke awareness clubs to organizing virtual conferences.

Conclusion
The MT2020+ student internship provided a novel opportunity for high school students to get exposure to global health implementation. The student research in this internship has contributed valuable insights into stroke treatment barriers and raised awareness among high school students worldwide.
Abstract 024: Endovascular Treatment of Cavernous Sinus Dural Arteriovenous Fistula: A Single Center experience
Authors: Mowla Ashkan , Abdollahifard Saeed , Taherifard Erfan , Szeder Viktor , Tateshima Satoshi , Nour May , Colby Geoffrey , Jahan Reza , Duckwiler Gary , Kaneko Naoki , Takayanagi Ariel , Liang Feng , Vinuela Fernando 
Published: March 2024

Introduction
The cavernous sinus dural arteriovenous fistula(CS‐DAVF) is a vascular condition that is a result of an unusual connection between the carotid artery branches and the cavernous sinus. This condition could be the result of trauma, infection, or other medical problems. In the case that conservative therapy fails or patients need emergency intervention, endovascular treatment (EVT) has been proposed as a main option for the treatment of these patients. In this study, we aimed to investigate the outcomes of the patients who underwent EVT for CS‐DAVF.

Methods
To gather information on patient demographics, symptoms, clinical and imaging characteristics, as well as the type of EVT and their outcomes after EVT, we recruited records of patients of our center from 2007. We used descriptive analyses to assess the characteristics of the included cases. Besides, inferential statistics were used to develop univariate and multivariate logistic regression models to identify factors linked to procedural success and symptom resolution at the follow‐up.

Results
Overall, 59 patients with a mean age of 64.37 years, a female‐to‐male ratio of 2.71, and a mean follow‐up time of 42.64 months were included. Chemosis, ptosis, and diplopia were the most frequent symptoms, and most of the patients were classified as Barrow classification type D based on angiographic features (30/58). In terms of EVT, most of the patients (54/59) underwent transvenous embolization, and liquids (Onyx 34 and 18) were the most frequent materials that were used for embolization (55/59). Also, in 34 cases, additional coiling was performed. Overall, the procedural success rate was 84% (50 out of 59), and a complete resolution of symptoms was observed in 46 cases at the follow‐up, with the occurrence of eight complications overall (13.5%), including two strokes, two alopecia, one seizure, one cranial nerve palsy, one diplopia and one worsening of symptom events. Multiple logistic regression showed that the use of Onyx 34 was associated with procedural success.

Conclusion
Our results showed that EVT might be a safe and effective option for the treatment of patients with CS‐DAVF. Also, the results showed that the application of Onyx 34 might associate with the procedural success rate.