bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of the Meditech ABPM-06 24-hour blood pressure monitoring system in a pediatric population according to International Organization for Standardization Protocol 81060-2:2018
Authors: Jakab Andrea E. , Horváth Edit , Molnár Dorottya , Bukva Mátyás , Bereczki Csaba 
Published: March 2024

Objective
Ambulatory blood pressure monitoring (ABPM) devices play a crucial role in diagnosing hypertension, not only in adults but also in pediatric patients. ABPM-06, the latest oscillometric device from Meditech Ltd. (Budapest, Hungary), is the focus of this study. The objective was to validate the ABPM-06 device using the International Organization for Standardization (ISO) 81060-2 : 2018 standard.

Methods
A total of 86 healthy patients, consisting of 34 males and 52 females, aged between 3 and 17 years, were enrolled in this study. During the ambulatory phase, a total of 50 patients were enrolled, with 35 patients falling within the 3- to 12-year-old age range and 15 patients aged between 12 and 17 years. Additionally, for the dynamic test, 36 patients were selected, comprising of 10 individuals aged 3–12 years and 26 patients aged 12–17 years. These patients were recruited from the outpatient clinics of the Department of Pediatrics at Albert Szent-Györgyi University in Szeged, Hungary. The validation process involved utilizing the same-arm sequence protocol, both in resting positions and during stress testing.

Results
The ABPM-06 performed well in both clinical and ambulatory validations. In terms of validation criterion 1, the mean ± SD of the differences between the test device and reference blood pressure readings was −1.3 ± 3.5  mmHg for systolic and −0.1  ± 2.3 mmHg for diastolic, in children under the age of 12 years. For those over the age of 12 years, the mean ± SD of the differences was −2.8 ± 4.6 mmHg for systolic and −0.5 ± 2.7 mmHg for diastolic. Regarding the ambulatory validation, for children under 12 years old, the mean ± SD of the differences was −1.3  ± 3.5  mmHg for systolic and −0.1  ± 2.3  mmHg for diastolic. In the age group above 12 years, the mean ± SD of the differences was −2.8 ± 4.6 mmHg for systolic and −0.5 ± 2.7 mmHg for diastolic. Both tests successfully met the established criteria regarding the mean and SD values of the differences between the device readings and the observed SBP and DBP measurements.

Conclusion
The ABPM-06 oscillometric device fully adheres to the ISO 81060-2 : 2018 standard requirements for ABPM determination in the pediatric population (ages 3–17 years). Consequently, this ABPM device proves to be suitable for effectively managing hypertension in children and adolescents.
Richter supraumbilical hernia managed with invagination: a case report
Authors: Dev Santosh , Luitel Prajjwol , Paudel Sujan , Devkota Shishir , Khadka Laxman , Jha Sanjeev , Dev Barsha , Neupane Nischal , Ghimire Bikal 
Published: March 2024

Introduction and importance:
Richter’s hernia is an incarceration of the anti-mesenteric border of a segment of bowel through an abdominal wall defect. It primarily affects elderly individuals but can occur at any age, with a slightly increased incidence in females. The increase in laparoscopic and robotic-assisted procedures has led to a rise in Richter’s hernias.

Case presentation:
A 40-year-old male with a history of laparoscopic cholecystectomy and kidney transplantation presented with a 4-day history of supraumbilical swelling and abdominal pain. The swelling was irreducible and accompanied by mild tenderness, and local signs of inflammation were exhibited. Intraoperatively, a 1.5 cm hernia defect was found, with the sac containing omentum and a portion of bowel segment for which invagination with serosal closure with the Mayo double-breasting technique was done.

Clinical discussion:
Richter’s hernia presents with abdominal discomfort, bloating, nausea, and vomiting, with a notable feature being the delayed onset of symptoms due to its partial involvement of the bowel wall. Diagnosis can be achieved through a computed tomography (CT) scan or intraoperative exploration. Management of Richter hernia is contingent upon the patient’s clinical condition, physical examination, and suspicion of strangulation.

Conclusion:
Diagnosis of Richter’s hernia demands higher suspicion, particularly in patients with predisposing factors like a history of minimally invasive surgery. Prompt surgical intervention is crucial for reducing mortality and enhancing prognosis, with invagination alone being adequate if ischaemia is confined and mesh placement is unnecessary.
Neuromuscular Electrical Stimulation Improves Frontal Ankle Motor Control in Individuals With Chronic Ankle Instability During Drop Landing
Authors: Zheng Huifen , Tian Fei , Sun Wei , Zheng Longpo , Xiao Weihua 
Published: March 2024

Objective
This study investigated the effect of neuromuscular electrical stimulation on the frontal ankle motor control in individuals with chronic ankle instability during drop landing.

Design
This was a randomized, controlled, double-blind trial. Thirty-six individuals with chronic ankle instability were randomly assigned to each group. Participants received 6-wk neuromuscular electrical stimulation intervention and sham stimulation in the neuromuscular electrical stimulation and control groups, respectively. Data were collected at week0 and week6. A mixed-effects model and analysis of covariance were employed to investigate the between-group differences in continuous and discrete outcome variables at week6, with the outcome variables at week0 as covariates.

Results
Compared to control group, neuromuscular electrical stimulation group exhibited a 2.66° (2.45, 2.86) reduction in frontal ankle inversion angle, a 47.41°/sec (−16.05, −78.77) decrease in peak ankle inversion angular velocity, and a 0.43 Nm/kg (0.18, 0.68) increase in peak ankle eversion moment during drop landing at week6.

Conclusions
Applying 6-wk neuromuscular electrical stimulation to the fibularis longus resulted in decreased ankle inversion angle and ankle inversion angular velocity and increased peak ankle eversion moment during drop landing. Consequently, neuromuscular electrical stimulation could be considered an effective modality for individuals with chronic ankle instability to enhance the frontal ankle movement patterns and overall ankle motor control.
Do HIV Care Outcomes Differ by Provider Type?
Authors: Weiser John , Tie Yunfeng , Crim Stacy M. , Riedel David J. , Shouse R. Luke , Dasgupta Sharoda 
Published: March 2024

Background:
We compared HIV care outcomes by HIV provider type to inform efforts to strengthen the HIV provider workforce.

Setting:
U.S.

Methods:
We analyzed data from CDC’s Medical Monitoring Project collected during 6/2019-5/2021 from 6,323 adults receiving HIV medical care. Provider types were infectious disease physicians only (ID physicians), non-ID physicians only (non-ID physicians), nurse practitioners only (NPs), physician assistants only (PAs), and ID physicians plus NPs and/or PAs (mixed providers). We measured patient characteristics, social determinants of health (SDOH), and clinical outcomes including retention in care; antiretroviral therapy prescription; antiretroviral therapy adherence; viral suppression; gonorrhea, chlamydia, and syphilis testing; satisfaction with HIV care; and HIV provider trust.

Results:
Compared with patients of ID physicians, higher percentages of patients of other provider types had characteristics and SDOH associated with poor health outcomes and received HIV care at Ryan White HIV/AIDS Program-funded facilities. After accounting for these differences, most outcomes were not meaningfully different, however higher percentages of patients of non-ID physicians, NPs, and mixed providers were retained in care (6.5, 5.6, and 12.7 percentage points, respectively) and had STI testing in the past 12 months, if sexually active (6.9, 7.4, and 13.5 percentage points, respectively).

Conclusion:
Most HIV outcomes were equivalent across provider types. However, patients of non-ID physicians, NPs, and mixed providers were more likely to be retained in care and have recommended STI testing. Increasing delivery of comprehensive primary care by ID physicians and including primary care providers in ID practices could improve HIV primary care outcomes.
68Ga-FAPI-04 PET/CT for the Evaluation of Cholangiocarcinoma
Authors: Zhang Zeyu , Cheng Chao , Jiang Hui , Pan Guixia , Yu Yong , Jin Gang , Zuo Changjing 
Published: March 2024

Purpose
In this study, we evaluated and compared the diagnostic performances of 68Ga-FAPI-04 PET/CT and 18F-FDG PET/CT for primary and metastatic cholangiocarcinoma (CCA) lesions. We also investigated the performance of PET/MR for visualizing and characterizing CCA and liver metastasis lesions.

Patients and Methods
Forty-four patients with suspected CCA were recruited and underwent 68Ga-FAPI-04 and 18F-FDG PET/CT within 1 week, including 30 patients who underwent simultaneous abdominal 68Ga-FAPI-04 PET/MR scanning. The findings were confirmed by histopathology or radiographic follow-up.

Results
Compared with 18F-FDG PET/CT, 68Ga-FAPI-04 PET/CT showed higher sensitivity (94.3% vs 88.6%) and the same accuracy (86.4% vs 86.4%) in evaluating primary tumors. However, its specificity was lower (55.6% vs 77.8%). 68Ga-FAPI-04 PET was superior to 18F-FDG PET in both patient-based and lesion-based evaluations except for metastatic lesions in the liver and bone. For intrahepatic CCA, 68Ga-FAPI-04 PET/CT and 18F-FDG PET/CT (100% vs 100%) had similar detection rates, with similar uptake levels between tracers (P > 0.05). However, for extrahepatic CCA, 68Ga-FAPI-04 PET/CT had a higher detection rate (89.5% vs 78.9%), and 68Ga-FAPI-04 had a higher uptake (P < 0.05). PET/MR was more effective than PET/CT in terms of lesion conspicuity and diagnostic confidence for primary tumors and liver metastases. In addition, multisequence MRI identified more liver metastases than 68Ga-FAPI-04 PET/CT and 18F-FDG PET/CT.

Conclusions
Compared with 18F-FDG PET/CT, 68Ga-FAPI-04 PET/CT showed a higher sensitivity in detecting primary CCA tumors, involved lymph nodes, and peritoneal metastases. Compared with 68Ga-FAPI-04 PET/CT, PET/MR detected primary and liver metastatic lesions more accurately. For extrahepatic CCA, the combination of 68Ga-FAPI-04 PET/CT and abdominal PET/MRI may replace 18F-FDG PET/CT.
Additional Findings of 18F-AIF-FAPI-42 PET/CT in a Patient With Mycosis Fungoides–Type Cutaneous T-Cell Lymphoma
Authors: Liu Guanghui , Li Yaming , Du Bulin , Cui Yan , Li Xuena 
Published: March 2024

Abstract
A 44-year-old woman presented with extensive skin patches and pruritus persisting for 3 years. Histopathological examination of the skin from the right abdomen confirmed mycosis fungoides–type cutaneous T-cell lymphoma. Staging PET with 18F-FDG PET/CT) showed increased uptake in the skin on the right abdomen and left hip. Subsequently 18F-FAPI-42 PET/CT revealed additional foci of abnormal uptake on the skin of the chest and back.
Widespread Reactive Lymphatic and Splenic FDG Avidity After mRNA COVID Vaccination
Authors: Kavanaugh Michael , Shah Hina J. 
Published: March 2024

Abstract
A 75-year-old woman with history of metastatic lung adenocarcinoma in remission develops new widespread FDG-avid lymphadenopathy in the neck, chest, abdomen, and pelvis on surveillance PET/CT, as well as intense FDG uptake in the spleen, without evidence of local recurrence. Short-term follow-up PET demonstrates near-complete resolution of FDG-avid lymphatic and splenic FDG avidity without interval change in management. Further history reveals that the patient received her fifth dose of COVID mRNA vaccine 6 days before the abnormal PET. Although unilateral axillary adenopathy after COVID vaccination is well-recognized, this widespread symmetric lymphatic and splenic FDG avidity is a significantly rarer phenomenon.
Disparities Research for Pelvic Floor Disorders: A Systematic Review and Critique of Literature
Authors: Mou Tsung , Shinnick Julia , DeAndrade Samantha , Roselli Nicole , Andebrhan Sarah , Akanbi Tracey , Ackenbom Mary , Carter-Brooks Charelle , Beestrum Molly , Cichowski Sara , Brown Oluwateniola 
Published: March 2024

Importance
Understanding the status of pelvic floor disorder (PFD) disparities research will allow the opportunity to advance future pelvic floor equity efforts.

Objectives
The aims of the study were to (1) characterize the landscape of PFD disparities literature using the 3 phases of disparities research framework, (2) describe the characteristics of PFD disparities studies, and (3) identify critical knowledge gaps.

Study Design
We performed a systematic review of peer-reviewed publications addressing disparities in PFDs among U.S. populations from PubMed, Embase, Scopus, or the Cochrane Database indexed between 1997 and 2022. Using the triphasic framework for advancing health disparities research by Kilbourne et al (Am J Public Health. 2006;96(12):2113–21), we categorized the included studies into the detecting phase (identifies and measures disparities in historically marginalized populations), understanding phase (establishes disparity determinants), or reducing phase (conducts interventions to alleviate inequities). All screening, coding, and quality reviews were independently performed by at least 2 authors. We used descriptive analysis and the χ2 test for comparisons.

Results
The initial search identified 10,178 studies, of which 123 were included. Of the included studies, 98 (79.7%), 22 (17.9%), and 3 (2.4%) studies were detecting, understanding, and reducing phase research, respectively. The most common disparity category investigated was race and ethnicity (104 studies), and one third of these studies attributed drivers of racial and ethnic differences to structural influences. Publications of detecting phase studies outpaced the growth of understanding and reducing phase research.

Conclusions
Most PFD disparities research focused on identifying historically marginalized populations with inadequate progression to understanding and reducing phases. We recommend progressing PFD disparities research beyond the detecting phase to advance health equity in PFD care.
Modelling Glioma Stem Cell-mediated Tumorigenesis Using Zebrafish PDX Systems
Authors: Mahendran Hema Priya , Cieslukowski Alan , Lubanska Dorota , Philbin Nicholas , Stringer Keith Franklin , Habashy Philip , Stover Mat , deCarvalho Ana C. , Soliman Mohamed , Shamisa Abdalla , Porter Lisa 
Published: March 2024

SummaryGlioblastoma is an aggressive brain tumour associated with high post-therapy recurrence and very poor survival rates. One of the factors contributing to the aggressive nature of this disease is the level of heterogeneity seen at the phenotypic and genetic level. Glioma Stem Cells (GSCs) are stem-like cells within the tumour with the ability to self-renew and give rise to different types of cells within the tumour, hence giving rise to the heterogeneity found in glioblastoma. GSCs are often implicated in the resistance of glioma to standard of care radiation and chemotherapy. The physical niche within a tumour mass supports stemness and aggressive characteristics of GSCs, hence, experimental systems providing a relevant tumour microenvironment are critical for adequate assessment of molecular mechanisms regulating GSC populations. Although, mouse models are a staple of anin vivoexperimental design, they are neither time-nor cost-efficient.Danio rerio(zebrafish) patient-derived xenografts (PDXs) overcome several of the obstacles of the mammalian systems. Zebrafish constitute a high throughput, easily reproducible experimental platform allowing for life relevant investigation into the aggressiveness of GSC populations. This chapter describes methods required for generation of zebrafish PDXs to study aspects of GSC-mediated tumorigenesis and interactions with the tumour microenvironment. Consistency between labs for these experiments is required to move the discovery of effective treatments for glioblastoma moving forward.
Histology-guided mathematical model of tumor oxygenation: sensitivity analysis of physical and computational parameters
Authors: Ojwang’ Awino Maureiq E. , Bazargan Sarah , Johnson Joseph O. , Pilon-Thomas Shari , Rejniak Katarzyna A. 
Published: March 2024

ABSTRACTA hybrid off-lattice agent-based model has been developed to reconstruct the tumor tissue oxygenation landscape based on histology images and simulated interactions between vasculature and cells with microenvironment metabolites. Here, we performed a robustness sensitivity analysis of that model’s physical and computational parameters. We found that changes in the domain boundary conditions, the initial conditions, and the Michaelis constant are negligible and, thus, do not affect the model outputs. The model is also not sensitive to small perturbations of the vascular influx or the maximum consumption rate of oxygen. However, the model is sensitive to large perturbations of these parameters and changes in the tissue boundary condition, emphasizing an imperative aim to measure these parameters experimentally.
Obstacle Negotiation in Female Locust Oviposition Digging
Authors: Klechevski Chen , Kats Lazar , Ayali Amir 
Published: March 2024

AbstractThe female locust lays its eggs deep within soft substrate to protect them from predators and provide optimal conditions for successful hatching. During oviposition digging, the female’s abdomen extends into the ground, guided by a dedicated excavation mechanism at its tip, comprising two pairs of specialized digging valves. Little is known about how these active valves negotiate the various obstacles encountered on their path. In this study, female locusts oviposited their eggs in specialized sand-filled tubes with pre-inserted 3D-printed plastic obstacles. The subterranean route taken by the abdomen and digging valves upon encountering the obstacles was investigated, characterized, and compared to that in control tubes without obstacles. Data were obtained by way of visual inspection, by utilizing Cone Beam Computed Tomography scans in high-definition mode, and by making paraffin casts of the oviposition burrows (after egg hatching). We demonstrate, for the first time, the subterranean navigation ability of the female locust’s excavation mechanism and its ability to circumvent obstacles during oviposition. Finally, we discuss the role of active sensory-motor mechanisms versus the passive embodied function of the valves, central control, and decision-making.
Dynamic energy budget model for a bumble bee colony: Predicting the spatial distribution and dynamics of colonies across multiple seasons
Authors: Capera-Aragones Pau , Mariño Joany , Hurford Amy , Tyson Rebecca C. , Foxall Eric 
Published: March 2024

AbstractBumble bees are important pollinators of many crops around the world. In recent decades, agricultural intensification has resulted in significant declines in bumble bee populations and the pollination services they provide. Empirical studies have shown that this trend can be reversed, however, by enhancing the agricultural landscape with natural habitat, such as adding wildflower patches adjacent to crops. Despite the empirical evidence, the mechanisms behind these positive effects are not fully understood, and the specific characteristics of the enhanced natural habitat that would maximize benefits are unclear at this time. Theoretical studies, in the form of mathematical models, have proven useful in elucidating the underlying mechanisms and determining the optimal natural habitat configurations. Existing models, however, generally focus only on particular aspects of bumble bee behaviour; some models are accurate at describing population dynamics, while others are accurate at describing their spatial distribution. In this work, we build a unique model coupling population dynamics, using a whole-colony Dynamic Energy Budget (DEB) approach, to a spatial distribution model based on the maximum energy principle. This coupling gives valuable new insights into the effects of spatial arrangements on population dynamics, and vice-versa. With our model, we answer questions such as when, how much, or what type of wildflower patches should be planted to maximize crop pollination services and minimize bee decline. We find that planting wildflowers that bloom before and after crop bloom is crucial to achieve high pollination services and preserving wild pollinator populations. We also find that small quantities of natural habitat are needed when the crop is nutritionally rich, but higher quantities are most beneficial when the crop is nutritionally deficient.
Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignment
Authors: Kovaka Sam , Hook Paul W. , Jenike Katharine M. , Shivakumar Vikram , Morina Luke B. , Razaghi Roham , Timp Winston , Schatz Michael C. 
Published: March 2024

AbstractNanopore signal analysis enables detection of nucleotide modifications from native DNA and RNA sequencing, providing both accurate genetic/transcriptomic and epigenetic information without additional library preparation. Presently, only a limited set of modifications can be directly basecalled (e.g. 5-methylcytosine), while most others require exploratory methods that often begin with alignment of nanopore signal to a nucleotide reference. We present Uncalled4, a toolkit for nanopore signal alignment, analysis, and visualization. Uncalled4 features an efficient banded signal alignment algorithm, BAM signal alignment file format, statistics for comparing signal alignment methods, and a reproduciblede novotraining method for k-mer-based pore models, revealing potential errors in ONT’s state-of-the-art DNA model. We apply Uncalled4 to RNA 6-methyladenine (m6A) detection in seven human cell lines, identifying 26% more modifications than Nanopolish using m6Anet, including in several genes where m6A has known implications in cancer. Uncalled4 is available open-source atgithub.com/skovaka/uncalled4.
Lipid Peroxidation and Type I Interferon Coupling Fuels Pathogenic Macrophage Activation Causing Tuberculosis Susceptibility
Authors: Yabaji Shivraj M. , Zhernovkov Vadim , Araveti Prasanna Babu , Lata Suruchi , Rukhlenko Oleksii S. , Abdullatif Salam Al , Vanvalkenburg Arthur , Alekseev Yuriy , Ma Qicheng , Dayama Gargi , Lau Nelson C. , Johnson W. Evan , Bishai William R. , Crossland Nicholas A. , Campbell Joshua D. , Kholodenko Boris N. , Gimelbrant Alexander A. , Kobzik Lester , Kramnik Igor 
Published: March 2024

AbstractA quarter of human population is infected with Mycobacterium tuberculosis, but less than 10% of those infected develop pulmonary TB. We developed a genetically defined sst1-susceptible mouse model that uniquely reproduces a defining feature of human TB: the development of necrotic lung granulomas and determined that the sst1-susceptible phenotype was driven by the aberrant macrophage activation. This study demonstrates that the aberrant response of the sst1-susceptible macrophages to prolonged stimulation with TNF is primarily driven by conflicting Myc and antioxidant response pathways leading to a coordinated failure 1) to properly sequester intracellular iron and 2) to activate ferroptosis inhibitor enzymes. Consequently, iron-mediated lipid peroxidation fueled IFNβ superinduction and sustained the Type I Interferon (IFN-I) pathway hyperactivity that locked the sst1-susceptible macrophages in a state of unresolving stress and compromised their resistance to Mtb. The accumulation of the aberrantly activated, stressed, macrophages within granuloma microenvironment led to the local failure of anti-tuberculosis immunity and tissue necrosis. The upregulation of Myc pathway in peripheral blood cells of human TB patients was significantly associated with poor outcomes of TB treatment. Thus, Myc dysregulation in activated macrophages results in an aberrant macrophage activation and represents a novel target for host-directed TB therapies.