bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological surveillance and phylogenetic diversity of Orthohantavirus hantanense using high-fidelity nanopore sequencing, Republic of Korea
Authors: Park Kyungmin , Kim Jongwoo , Noh Juyoung , Kim Seong-Gyu , Cho Hee-Kyung , Kim Kijin , Seo Ye-rin , Lim Taehun , Lee Seonghyeon , Lee Jaeyeon , Lim Seung In , Joo Young Hoon , Lee Buddle , Yun Seok Hyeon , Park Changbo , Kim Won-Keun , Song Jin-Won 
Published: February 2025

Background
Orthohantavirus hantanense (HTNV) poses a substantial global public health threat due to its role in causing hemorrhagic fever with renal syndrome (HFRS). HTNV outbreaks are particularly prevalent in the Gyeonggi and Gangwon Provinces of the Republic of Korea (ROK). This study aimed to evaluate the application of advanced nanopore sequencing and bioinformatics to generate complete genome sequences of HTNV, with the objective of accurately identifying infection sources and analyzing their genetic diversity.

Methodology/Principal findings
In 2022 and 2023, we collected 579 small mammals from 11 distinct locations across Gyeonggi and Gangwon Provinces, as well as in Gwangju Metropolitan City, ROK. Among these, 498 Apodemus agrarius specimens were subjected to an epidemiological survey to investigate HTNV infections. The serological and molecular positivity of HTNV were found to be 65/498 (13.1%) and 17/65 (26.2%), respectively. Furthermore, 15 whole-genome sequences of HTNV were obtained from rodents in Gyeonggi and Gangwon Provinces. We developed a novel amplicon-based nanopore sequencing approach to acquire high-fidelity and precise genomic sequences of HTNV. Genome exchange analysis revealed three reassortant candidates, including heterogeneous L segments, from Paju-si and Yeoncheon-gun in Gyeonggi Province.

Conclusion/Significance
Our findings enhance the resolution of the spatiotemporal genomic surveillance of HTNV by consistently providing new viral sequences and epidemiological data from HFRS-endemic regions in the ROK. This report signifies a notable advancement in nanopore sequencing techniques and bioinformatics, offering a robust platform for genome-based diagnostics and sophisticated phylogenetic analyses of orthohantaviruses, which are essential for public health strategies aimed at controlling HFRS.
Beijing’s Global Media Offensive: China’s Uneven Campaign to Influence Asia and the World, Joshua Kurlantzick (2022)
Authors: Zhang Li , Liu Zhaoning 
Published: February 2025

Review of: Beijing’s Global Media Offensive: China’s Uneven Campaign to Influence Asia and the World, Joshua Kurlantzick (2022)
Oxford: Oxford University Press, 560 pp.,
ISBN 978-0-19751-576-1, h/bk, £27.99
A Family With Multiple Lynch Syndrome Mutations: Navigating Counseling Complexities
Authors: Silva-Smith Rachel , Manso Gretter , Sussman Daniel Andrew 
Published: February 2025

ABSTRACT
Families with multiple Lynch syndrome pathogenic variants present unique challenges in genetic counseling and clinical management. We report a family with pathogenic variants in multiple mismatch repair genes identified through multigene panel testing. Key issues highlighted by this case include recognizing when comprehensive genetic testing is necessary, tailoring management to specific genetic mutations, and ensuring accurate communication. This case highlights potential pitfalls in risk assessment and counseling for complex Lynch syndrome families.
Broadened substrate specificity of bacterial dipeptidyl-peptidase 7 enables release of half of all dipeptide combinations from peptide N-termini
Authors: Shirakura Kana , Nemoto Takayuki K. , Nemoto Yuko Ohara , Nishimata Haruka , Sawase Momo , Shimoyama Yu , Nakasato-Suzuki Manami , Ito Kiyoshi , Tanoue Naomi 
Published: February 2025

Abstract
Dipeptide production mediated by dipeptidyl-peptidase (DPP)4, DPP5, DPP7, and DPP11 plays a crucial role in growth of Porphyromonas gingivalis, a periodontopathic asaccharolytic bacterium. Given the particular P1-position specificity of DPPs, it has been speculated that DPP5 or DPP7 might be responsible for degrading refractory P1 amino acids, i.e., neutral (Thr, His, Gly, Ser, Gln) and hydrophilic (Asn) residues. The present results identified DPP7 as an entity that processes these residues, thus ensuring complete production of nutritional dipeptides in the bacterium. Activity enhancement by the P1′ residue was observed in DPP7, as well as DPP4 and DPP5. Toward the refractory P1 residues, DPP7 uniquely hydrolyzed HX|LD-MCA (X = His, Gln, or Asn) and their hydrolysis was most significantly suppressed in dpp7 gene-disrupted cells. Additionally, hydrophobic P2 residue significantly enhanced DPP7 activity toward these substrates. The findings propose a comprehensive 20 P1 × 20 P2 amino acid matrix showing the coordination of four DPPs to achieve complete dipeptide production along with subsidiary peptidases. The present finding of a broad substrate specificity that DPP7 accounts for releasing 48 % (192/400) of N-terminal dipeptides could implicate its potential role in linking periodontopathic disease to related systemic disorders.
Evolution and Future of the Competitive Advantages of Wine-Producing Countries
Authors: Bao Alex , Cardebat Jean-Marie , Chauvin Marie 
Published: February 2025

Abstract
This paper discusses international trade prospects of the main wine-producing countries over different time horizons. Our framework is composed of two approaches and aims at renewing and extending the findings of the existing literature on the dynamics of major wine exporters. Firstly, we apply a Porter’s diamond analysis in order to disentangle competitive advantages in 16 countries from various features of the global wine market. Then, we assess short- and medium-term prospects from the evolution of past trends. Secondly, we use data from a survey of (57) wine experts as a robustness analysis to complete the prospective dimension over the long term. Compared with previous literature (prior to the Covid and Ukraine shocks), our results show that the international competitiveness of countries has significantly changed over the last five years. France and Italy should maintain their leadership, but Spain and Chile are slipping back, while China could collapse. New Zealand could eventually become a major leader. These results indicate that competitive advantages are very dynamic in the wine industry, despite static natural endowments (excluding climate). Investment incentives and innovation should therefore play a key role in the long term in this sector, which is often presented as being driven by tradition and natural factors.
Determining the effects of social media engagement on surgery residents within the American College of Osteopathic Surgeons
Authors: Alexander Vincent S. , Eke Bebe , Xu Andrew , Wong Ryan , Greek Andrew , Ernst Michael , Roberts Hayden , Ariwodo Ogechukwu , Vogel Andrew D. , Burns Bracken , Conrad-Schnetz Kristen 
Published: February 2025

Abstract

Context
The widespread adoption of social media has transformed how individuals connect in medicine. This study explores how social media can be utilized as a conduit to connect osteopathic medical students and residents. By examining how these digital connections can support professional development, this study investigates social media’s role as a potential foundation for a mentorship program, fostering guidance, support, and career insights for osteopathic medical students within the surgical field.

Objectives
This study aimed to evaluate the effectiveness of “X” (formerly Twitter) in networking between osteopathic medical students and surgical residents, specifically assessing engagement rates and the potential of social media as a tool for mentorship in the osteopathic surgical community.

Methods
The Accreditation Council on Graduate Medical Education (ACGME)-approved surgical programs that the American College of Osteopathic Surgeons (ACOS) represents were identified online. Doctor of Osteopathic Medicine (DO) residents were identified retrospectively through an online search of publicly available data. Residents and programs were searched through X, and account handles were collected. Residents were contacted through X by direct messaging and subsequently directly commented in the initial ACOS Medical Student Section (ACOS-MSS) message. Descriptive statistics along with the chi-square and Fisher’s exact statistical tests were utilized for demographic and categorical variable analysis, respectively.

Results
There are 1179 DO surgical residents in ACGME programs, with 84.8 % of those being General Surgery residents. Among residents, 75 % of Cardiothoracic (CT) Surgery, 46.4 % of Urology, 28.6 % of Neurosurgery, 17.3 % of General Surgery, and 11.11 % of Plastic Surgery residents have X accounts. Specific surgical subspecialties are more likely to have an X account in comparison to General Surgery, including CT Surgery (odds ratio [OR], 11.13; p=0.02), Urology (OR, 4.13; p<0.0001), and Neurosurgery (OR, 0.842; p=0.047). After multiple attempts, 0 % of Plastic Surgery, 2.9 % of General Surgery, 7.8 % of Urology, 18.8 % of Neurosurgery, and 33.3 % of CT Surgery residents signed up for the mentorship program, totaling 13 residents.

Conclusions
The study reveals low social media engagement via X among osteopathic surgical residents, with notable variation across surgical specialties. This limited engagement suggests exploring and refining social media strategies that foster mentorship connections within the osteopathic surgical community. Future studies should examine other social media platforms and alternative engagement approaches to enhance the reach and impact of mentorship initiatives, ultimately supporting osteopathic medical students as they navigate career paths in surgery.
Spectro-FLIM for heritage: scanning and analysis of the time resolved luminescence spectra of a fossil shrimp
Authors: Patarin Louis , Ritoux Jérémie , Audibert Jean-Frédéric , Bertrand Loïc , Brayard Arnaud , Fara Emmanuel , Pansu Robert B. 
Published: February 2025

Abstract
We describe the use of a single-photon counting imaging device to scan the surface of a flat fossil specimen. We studied an Early Triassic shrimp identified as Anisaeger longirostrus, from the Paris Biota (Idaho, USA; ca 249.1 Ma). Chemically, the specimen consists of a polycrystalline material composed of relatively monodisperse transparent crystals about 10 µm in size. We collected time-resolved fluorescence spectra at each pixel along 300 µm wide ribbons at a rate of 10 µm per second with a spatial resolution of 1 µm × 10 µm. Fluorescence is excited along a line. We demonstrate the presence of two types of crystals whose luminescence significantly differs. The first type emits at 450 nm with a decay time of 840 ps. The second type contains two independent emission centres emitting at 437 nm and 490 nm. They exhibit multiexponential decays with lifetimes of 1 ns and more than 5 ns, respectively. Although a variable proportion of long emitters was observed, the fluorescence of the fossil appeared to be relatively uniform. Thus, over time, fossilization processes have therefore resulted in a homogeneous distribution of fairly pure crystals.
Cutting-edge developments in agro-waste derived carbon nanomaterials and their multifaceted applications in agriculture: enhancing crop imaging, nutrient efficiency, and plant health
Authors: Bhattacharya Tanima , Das Tanmoy , Preetam Subham , Chopra Hitesh 
Published: February 2025

Abstract
The rising global population is a contributing factor to the exponential rise of agricultural output. Agro-wastes are now recognized as a substitute substance to produce sustainable, affordable, and renewable bio-based products. Abundant farm products due to a lack of processing capacity or storage room are frequently lost. A substantial amount biopolymer is present in agricultural residues. It’s interesting that nanotechnology has the potential to more easily convert agricultural wastes into valuable and affordable products, eliminating the need to use toxic chemicals, which can lead to a variety of health and environmental problems. Recently, there has been a rise in interest in environmentally beneficial carbon nanomaterial (CNM) manufacturing methods that make use of agricultural waste extracts. Therefore, the goal of this review is to look into the various agro-waste sources, synthesis techniques of CNMs, characterization methods, surface functionalization chemistry, mechanistic approach and potential applications in imaging crops, nutrient utilization, and upgradation of crop health.
In vivo evaluation of the antinociceptive effects of novel methylsulfonyl group-containing compounds
Authors: Eken Hazal , Akgöl Jale , Kurban Berkant , Osmaniye Derya 
Published: February 2025

Abstract
Pain management is among the problems that remain important today, and analgesic agents such as nonsteroidal anti-inflammatory drugs (NSAIDs), opioids, tricyclic antidepressants, and anticonvulsants used in pain treatment cause various side effects. Therefore, new, safe, and highly effective drugs are needed. Synthesis of chemical molecules has begun to be preferred as a faster and easier method in new drug development processes. In this study, new compounds (3a–3k) containing the “methylsulfonyl group,” which have been shown to have potential inhibitory effects on cyclooxygenase (COX) enzymes responsible for the synthesis of inflammatory mediators, were synthesized. Central antinociceptive effect of the compounds was evaluated by hot-plate and tail-immersion tests, and the peripheral antinociceptive effect was evaluated by acetic acid-induced writhing test. 3a, 3b, 3d, 3e, 3f, 3g, 3h, 3i, 3j, and 3k (20 mg/kg) significantly increased the percentage of the maximum possible effect (MPE %) in the hot-plate test compared to the control group. 3a–3k (20 mg/kg) significantly increased MPE % in the tail-immersion test compared to the control group and significantly decreased the number of writhings in the acetic acid-induced writhing test. The results of this study indicate that the synthesized compounds containing methylsulfonyl groups have central and peripheral antinociceptive effects.
Using Position, Velocity, and Acceleration Relationships to Assess Exercise Specificity: A Simple Tool for the Strength Coach Toolbox
Authors: Harry John R. , Hite Mia , Simms Anton , Park Sunny , Stewart Michael , Barker Leland A. 
Published: February 2025

ABSTRACT
Training specificity using the dynamic correspondence approach is effective for athletic development because it emphasizes specific movement outcomes and influential strategies. Unfortunately, limited technological resources and formal training can restrict strength and conditioning or sport science professionals from applying research-driven data acquisition and interpretation approaches, creating a reliance on qualitative approaches to assess specificity. However, the dynamic correspondence form of specificity is rooted in mechanics, meaning coaches can often reframe their focus to the motions and accelerations occurring at the joint or whole body (i.e., center of mass) level. These motions can be observed for target exercises, which are the ones we hope to improve during testing or performance, and for the potential training exercises. From those observations, practitioners can create a profile using simple sketches and an understanding of the conceptual relationships among position, velocity, and acceleration (PVA). The article explains the conceptual PVA relationships and demonstrates the PVA profiling process for 2 exemplar scenarios. The simplicity of the PVA profile can provide an additional approach for identifying sport-specific exercises or even within-exercise qualities, such as time periods with high or low acceleration. Strength and conditioning professionals may benefit from supplementing current approaches to specificity determinations with PVA profiles.
Pre-gestational diabetes in a young woman with a pathogenic INSR missense mutation, p.(Met1180Lys)
Authors: Prehn Emma L , Crowley Mairéad , Fennell David , Kinsley Brendan T , Colclough Kevin , Byrne Maria M 
Published: February 2025

Summary
Heterozygous insulin receptor (INSR) mutations cause type A insulin resistance (IR), associated with a phenotype of IR; hyperandrogenism, oligomenorrhoea and acanthosis nigricans in the absence of obesity or lipoatrophy. The phenotype is variable, ranging from neonatal hyperinsulinaemic hypoglycaemia to fasting or post-prandial hypoglycaemia in adults to diabetes. We report a 29-year-old woman presenting at 13 weeks gestation in her second pregnancy. Diabetes was diagnosed at 13-years-old following presentation with lethargy and polyuria and she was treated with metformin 500 mg po bd. She also had polycystic ovarian syndrome, hypothyroidism and epilepsy. Metformin was changed to insulin with good glycaemic control throughout pregnancy. She delivered a 3.95 kg male infant at 39 weeks gestation without neonatal hypoglycaemia. At 18 months post-partum, her body mass index was 26.3 kg/m2, with no evidence of acanthosis nigricans or features of lipodystrophy. As her sister was also diagnosed with diabetes at 13-years-old, next-generation sequencing was performed for known maturity onset diabetes of the young (MODY) genes and a p.(Met1180Lys) mutation in the INSR gene was detected. She reported nocturnal hypoglycaemia and a 5-h oral glucose tolerance test revealed post-prandial hyperinsulinaemic hypoglycaemia at 210 min. Her subsequent pregnancy was spontaneously treated with metformin 500 mg po od from 8 to 25 weeks gestation and discontinued due to intrauterine growth restriction. She delivered a 1.8 kg female infant at 34 plus 3 weeks gestation (25th centile) via elective caesarean section. The infant had transient neonatal hypoglycaemia for two days. Post-partum, she remains diet controlled, with a haemoglobin A1c of 32 mmol/mol. This case highlights the importance of genetic testing to establish optimal diabetes treatment.

Learning points

This case highlights the less severe phenotype of IR in a subject with an INSR p.Met1180Lys mutation.

It demonstrates the existence of symptomatic post-prandial hypoglycaemia in an adult subject associated with hyperinsulinaemia.

This case highlights the importance of genetic testing to establish diagnosis and allows for precision medicine.

The role of metformin use in Type A-IR and pregnancy needs to be established.
Seahorses as flagship species in the largest hypersaline lagoon in South America
Authors: Lopes Costa Leonardo , Mendonça Caio , Freitas Rangel Danilo 
Published: February 2025

The flagship species concept is based on the perceived "charisma" of species. While certain attributes have been proposed to predict charisma, support for conservation of flagship species is often driven by local and context-specific factors. The largest hypersaline lagoon in South America has been considered for restoration programs after suffering from decades of urban development and pollution. Recently, the seahorse Hippocampus reidi was reported in the lagoon, generating significant public attention. Inspired by marketing theory, we conducted a study to evaluate people's preferences for various species inhabiting the lagoon. In a choice experiment, participants were shown high-resolution photographs of ten species, including the seahorse, and asked to rank their top three preferred species (most preferred = 3, second most preferred = 2, and third most preferred = 1), as well as their three least preferred species (least preferred = -3, second least preferred = -2, and third least preferred = -1). We calculated a flagship score for each species based on these rankings and compared them using ANOVA. The seahorse had a significantly higher flagship score than all other species, with its intrinsic aesthetic beauty being the main reason for its preference according to participants. This interest in seahorses presents a unique opportunity to apply the flagship species concept as a conservation fundraising strategy, promoting a biocentric perspective of the largest hypersaline lagoon in South America.
Evaluation of Transformer-Based Large Language Models for Email Spam Detection Using BERT, Phi, and Gemma
Authors: C. Grassmann Ana Clara , C. Feitosa Juliana , F. Brega José Remo , A. P. da Costa Kelton 
Published: February 2025

In this paper, we study how LLMs based on the transformer architecture work and the possibility of adjusting these models to use only the body of email messages to classify them as spam or ham. The models studied are BERT, Gemma, and Phi. All of them underwent quantization stages, fine-tuning with a real dataset, and evaluation with metrics commonly used in binary classification problems. The Gemma model achieves over 99% accuracy in detecting spam, standing out as the best among the compared models.
A Comprehensive Life Cycle Assessment of Hydraulic Fracturing
Authors: Khan Abdul Muqtadir , Anderson Christine , Prabhu Rasika , Beletskaya Anna , Kuan Pearl , Danican Samuel , Mostowfi Farshid 
Published: February 2025

Summary
The oil and gas industry has been taking steps to achieve sustainable growth. Most exploration and production companies have declared their commitment to a carbon-neutral future. The first step in realizing this goal is to identify high-intensity operations, such as unconventional hydraulic fracturing, and reduce those emissions. Therefore, the primary objective of this work is to quantify the main sources of emissions in the hydraulic fracturing value chain and identify technologies that could dramatically reduce those emissions.
Our first primary goal is to identify nearly all sources of emissions and the scale of their impact. Once the main emission sources are identified in the entire unconventional fracturing chain, we then focus on technologies that enable significant emission reduction. We used qualitative and quantitative analysis to assess the emissions impact of diesel engines, material transportation, and water utilization. Our observations yielded a high-level view of emission intensity across the value chain of hydraulic fracturing. Wellsite execution–related fuel consumption during pumping was the highest contributor to emissions at 36%, followed closely by the flaring for fracture cleanup at 29%. Total transportation contributed 24% to the total emissions. While the emissions of the fracturing fleets are often very visible due to their large footprint and duration, surprisingly, the nonroutine flaring and transportation are also impactful. In fact, nonroutine flaring could have a much higher impact depending on how fast the cleanup process could take place, which may not be easy to predict.
It is reported that using natural gas in internal combustion engines reduces CO2 emissions. However, the methane leak from engines would offset the CO2 savings and render the benefits marginal. We also observed that the reuse of produced water is a feasible way to reduce the well stimulation footprint on water resources, which could be enabled by improved water treatment techniques at scale, advances in hydraulic fracturing fluid composition, and appropriate infrastructure. We quantified the upstream emissions (embodied carbon) of commonly used chemicals, and our calculations show that the embodied carbon of commonly used chemicals in unconventional fracturing is rather small. Our conclusions emphasize the importance of adopting ecofriendly technologies to address the challenges posed by hydraulic fracturing. They can impact up to 35% of emissions reduction.
This study contributes a high-level, yet accurate, perspective on emissions of the hydraulic fracturing process. We highlight the high-emitting steps in the process and identify technology gaps that could reduce the emission footprint of the industry. This research underscores the urgency of adopting responsible practices in hydraulic fracturing for a harmonious coexistence with global sustainability objectives.