bluuebunny/crossref_metadata_2025_split huggingface dataset:
Storage mycotoxin producing fungi in Hungarian sorghum (Sorghum bicolor L. Moench) samples—molecular approach of Fusarium spp.
Authors: Szabó Barbara Katalin , Körösi Katalin 
Published: March 2024

Abstract
The last few years have been a huge challenge for every farmer in Europe and Hungary because of the increase in hot days and the decrease in precipitation. These facts induced the farmer’s interest in sorghum because it has better stress tolerance than many other cereales, but little is known about mycotoxin-producing fungi, which can infect this crop. Mycotoxins are secondary metabolites of filamentous fungi, and they are not only phytotoxic but also harmful to humans and animals. This study aimed to determine the internal infection caused by Alternaria spp., Fusarium spp., and Aspergillus spp., which are known as mycotoxin-producing fungi in food raw material. In our study in the case of sorghum, the presence of various mycotoxin-producing fungi was detected on Fusarium selective media (Nash and Snyder media), and the results indicate that these genera are present in sorghum grains as potential mycotoxin producers. To determine which Fusarium species is occurring in our sorghum grain samples, a molecular genetic study was performed on isolated fungi using the TEF region primer pairs to identify the occurring Fusarium species. We mainly identified Fusarium proliferatum in our conventional sorghum samples. The results show that the mentioned mycotoxin-producing fungi are in the sorghum grains and may pose a risk to the safety of feed and food because they may produce mycotoxins in the field or while being stored.
Canadian agriculture technology adoption
Authors: Easher Tahmid Huq , Enstroem Rickard , Griffin Terry , Nilsson Tomas 
Published: March 2024

Abstract
Objectives
Statistics Canada administers the Agricultural Census every 5 years, and this paper presents unsuppressed data from the 2016 and 2021 Census. The data set encompasses detailed information on farm types, sizes, technology choices, and a demographic profile of farm operators from the 2021 Census. Data on farm characteristics and operator demographics is crucial for understanding innovation in agriculture and formulating evidence-based policies.

Data description
The data sets cover the two most recent agriculture censuses of 2016 and 2021, presenting data on the number of farmers by region, farm type, size, and the adoption of technologies. Additionally, a third data set lists the number of farm operators by age and sex. The census questionnaire inquires about using different technologies, varying the types across the two census periods. Notably, there is no data suppression in these data sets, and they cover all 10 provinces in Canada, excluding the three territories. Farm types are categorized based on the North American Industry Classification System (NAICS), and farm size is measured in acres.
The Tahitian chestnut [Inocarpus fagifer (Parkinson ex F.A.Zorn) Fosberg, Fabaceae], a neglected multi-purpose tree from the Asia–Pacific region
Authors: Müller Jonas V. 
Published: March 2024

AbstractThe Tahitian (or Polynesian) chestnut Inocarpus fagifer is distributed widely in tropical South-East Asia and in the Pacific region. It is an important component in traditional agroforestry systems. However, its importance as a food plant has decreased considerably. There is no evidence that the species has ever been domesticated, nor that deliberate breeding and crop improvement is presently taking place. This article aims to provide an overview of the current status of knowledge about its distribution, biology, its past and current cultivation and use as a species in agroforestry systems, and to analyse its future potential for production, commercial use and plant improvement. Inocarpus fagifer grows in lowlands in coastal and near coastal areas, often along rivers. It often forms almost pure stands underneath the canopy trees. Inocarpus fagifer trees are very vigorous and shade-tolerant. They thrive in moist to wet soils. Nearly all parts of the tree have been used for multiple purposes including timber, medicine, and animal fodder. The seed has significant traditional importance as human food, and it plays an important role in the diets of rural communities, in particular during the crop harvesting season. Inocarpus fagifer is mostly disease-free. Reports about the commercial use of I. fagifer remain localised and largely anecdotal. The species retains a good potential for commercial development and for a wider commercial use as an alternative food source. No accessible genebank accessions are available. Fundamental questions on its marketability and genetic potential need to be answered.
TWO-CARDINAL DERIVED TOPOLOGIES, INDESCRIBABILITY AND RAMSEYNESS
Authors: CODY BRENT , LAMBIE-HANSON CHRIS , ZHANG JING 
Published: March 2024

Abstract
We introduce a natural two-cardinal version of Bagaria’s sequence of derived topologies on ordinals. We prove that for our sequence of two-cardinal derived topologies, limit points of sets can be characterized in terms of a new iterated form of pairwise simultaneous reflection of certain kinds of stationary sets, the first few instances of which are often equivalent to notions related to strong stationarity, which has been studied previously in the context of strongly normal ideals. The non-discreteness of these two-cardinal derived topologies can be obtained from certain two-cardinal indescribability hypotheses, which follow from local instances of supercompactness. Additionally, we answer several questions posed by the first author, Holy and White on the relationship between Ramseyness and indescribability in both the cardinal context and in the two-cardinal context.
Increased cardiac vagal tone in childhood-only, adolescent-only, and persistently antisocial teenagers: the mediating role of low heart rate
Authors: Raine Adrian , Brodrick Lia , Pardini Dustin , Jennings J. Richard , Waller Rebecca 
Published: March 2024

AbstractBackgroundCardiac vagal tone is an indicator of parasympathetic nervous system functioning, and there is increasing interest in its relation to antisocial behavior. It is unclear however whether antisocial individuals are characterized by increased or decreased vagal tone, and whether increased vagal tone is the source of the low heart rate frequently reported in antisocial populations.MethodsParticipants consisted of four groups of community-dwelling adolescent boys aged 15.7 years: (1) controls, (2) childhood-only antisocial, (3) adolescent-only antisocial, and (4) persistently antisocial. Heart rate and vagal tone were assessed in three different conditions: rest, cognitive stressor, and social stressor.ResultsAll three antisocial groups had both lower resting heart rates and increased vagal tone compared to the low antisocial controls across all three conditions. Low heart rate partially mediated the relationship between vagal tone and antisocial behavior.ConclusionsResults indicate that increased vagal tone and reduced heart rate are relatively broad risk factors for different developmental forms of antisocial behavior. Findings are the first to implicate vagal tone as an explanatory factor in understanding heart rate – antisocial behavior relationships. Future experimental work using non-invasive vagus nerve stimulation or heart rate variability biofeedback is needed to more systematically evaluate this conclusion.
Effect of Yangxin Huoxue Jiedu recipe on inflammatory factors and oxidative stress on viral myocarditis in children
Authors: Hao Hengrui , Ji Meixia , Zhou Kuilong , Zhang Yunxia , Zhang Gaoyin , Ruan Lianying 
Published: March 2024

AbstractObjective:This observation purposed to investigate the effect of the Yangxin Huoxue Jiedu formula on children with viral myocarditis and its effect on inflammatory factors and oxidative response.Materials and methods:A total of 121 children with viral myocarditis were randomly divided into two groups, namely the control group (N = 60) and the traditional Chinese medicine group (N = 61). The control group was mainly treated with routine therapy, while the traditional Chinese medicine group was treated with Yangxin Huoxue Jiedu recipes based on the control group. The creatine kinase, creatine kinase myocardial isoenzyme, aspartate aminotransferase, lactic dehydrogenase, hydroxybutyrate dehydrogenase, cardiac troponin I, brain natriuretic peptide, interleukin-6, interleukin-8, and tumour necrosis factor-alpha, superoxide dismutase and malondialdehyde in viral myocarditis patients were tested to estimate the myocardial function, inflammation, and oxidative situation.Results:After Yangxin Huoxue Jiedu treatment, 15 cases were recovered, 20 were excellent, and 21 were effective, which had a significant difference from the control group. The concentration of creatine kinase, creatine kinase myocardial isoenzyme, aspartate aminotransferase, lactic dehydrogenase, hydroxybutyrate dehydrogenase, cardiac troponin I and brain natriuretic peptide was decreased in the traditional Chinese medicine group. The levels of interleukin-6, interleukin-8, and tumour necrosis factor-alpha in the traditional Chinese medicine group were significantly lower than those in the control group. Superoxide dismutase was higher and malondialdehyde was lower than those in the control group.Conclusion:The use of Yangxin Huoxue Jiedu in the treatment of viral myocarditis has a definite clinical effect, which could improve myocardial function, reduce body inflammation, and promote oxidative recovery.
Depth Dependent Dynamics Explain the Equatorial Jet Difference Between Jupiter and Saturn
Authors: Duer Keren , Galanti Eli , Kaspi Yohai 
Published: March 2024

AbstractJupiter's equatorial eastward zonal flows reach wind velocities of ∼100 m s−1, while on Saturn they are three times as strong and extend about twice as wide in latitude, despite the two planets being overall dynamically similar. Recent gravity measurements obtained by the Juno and Cassini spacecraft uncovered that the depth of zonal flows on Saturn is about three times greater than on Jupiter. Here we show, using 3D deep convection simulations, that the atmospheric depth is the determining factor controlling both the strength and latitudinal extent of the equatorial zonal flows, consistent with the measurements for both planets. We show that the atmospheric depth is proportional to the convectively driven eddy momentum flux, which controls the strength of the zonal flows. These results provide a mechanistic explanation for the observed differences in the equatorial regions of Jupiter and Saturn, and offer new understandings about the dynamics of gas giants beyond the Solar System.
Multiplexed fluorescence and scatter detection with single cell resolution using on-chip fiber optics for droplet microfluidic applications
Authors: Gupta Preksha , Mohan Ambili , Mishra Apurv , Nair Atindra , Chowdhury Neeladri , Balekai Dhanush , Rai Kavyashree , Prabhakar Anil , Saiyed Taslimarif 
Published: March 2024

AbstractDroplet microfluidics has emerged as a critical component of several high-throughput single-cell analysis techniques in biomedical research and diagnostics. Despite significant progress in the development of individual assays, multiparametric optical sensing of droplets and their encapsulated contents has been challenging. The current approaches, most commonly involving microscopy-based high-speed imaging of droplets, are technically complex and require expensive instrumentation, limiting their widespread adoption. To address these limitations, we developed the OptiDrop platform; this platform is a novel optofluidic setup that leverages the principles of flow cytometry. Our platform enables on-chip detection of the scatter and multiple fluorescence signals from the microfluidic droplets and their contents using optical fibers. The highly customizable on-chip optical fiber-based signal detection system enables simplified, miniaturized, low-cost, multiparametric sensing of optical signals with high sensitivity and single-cell resolution within each droplet. To demonstrate the ability of the OptiDrop platform, we conducted a differential expression analysis of the major histocompatibility complex (MHC) protein in response to IFNγ stimulation. Our results showed the platform’s ability to sensitively detect cell surface biomarkers using fluorescently labeled antibodies. Thus, the OptiDrop platform combines the versatility of flow cytometry with the power of droplet microfluidics to provide wide-ranging, scalable optical sensing solutions for research and diagnostics.
Myricetin alleviates diabetic cardiomyopathy by regulating gut microbiota and their metabolites
Authors: Zhu Jinxiu , Bao Zhijun , Hu Zuoqi , Wu Shenglin , Tian Cuihong , Zhou Yueran , Ding Zipeng , Tan Xuerui 
Published: March 2024

Abstract
Background
The gut microbiota is involved in the pathogenesis of diabetic cardiomyopathy (DCM). Myricetin protects cardiac function in DCM. However, the low bioavailability of myricetin fails to explain its pharmacological mechanisms thoroughly. Research has shown that myricetin has a positive effect on the gut microbiota. We hypothesize that myricetin improves the development of DCM via regulating gut microbiota.

Methods
DCM mice were induced with streptozotocin and fed a high-fat diet, and then treated with myricetin by gavage and high-fat diet for 16 weeks. Indexes related to gut microbiota composition, cardiac structure, cardiac function, intestinal barrier function, and inflammation were detected. Moreover, the gut contents were transplanted to DCM mice, and the effect of fecal microbiota transplantation (FMT) on DCM mice was assessed.

Results
Myricetin could improve cardiac function in DCM mice by decreasing cardiomyocyte hypertrophy and interstitial fibrosis. The composition of gut microbiota, especially for short-chain fatty acid-producing bacteria involving Roseburia, Faecalibaculum, and Bifidobacterium, was more abundant by myricetin treatment in DCM mice. Myricetin increased occludin expression and the number of goblet cells in DCM mice. Compared with DCM mice unfed with gut content, the cardiac function, number of goblet cells, and expression of occludin in DCM mice fed by gut contents were elevated, while cardiomyocyte hypertrophy and TLR4/MyD88 pathway-related proteins were decreased.

Conclusions
Myricetin can prevent DCM development by increasing the abundance of beneficial gut microbiota and restoring the gut barrier function.
Long term outcomes of nonmyeloablative allogeneic stem cell transplantation with TSEB TLI and ATG for Mycosis Fungoides and Sezary Syndrome
Authors: Morris S. L. , Thomas B. R. , Palanicawandar R. , Whittaker S. , Child F. , Wain M. , Sim V. , Szydlo R. , Mangar S. , Olavarria E. , Lozano Cerrada S. , Muzamil A. , Kanfer E. 
Published: March 2024

AbstractAdvanced stage (IIB-IVB) Mycosis Fungoides (MF) and Sezary Syndrome (SS) have a poor prognosis with median survival <5 years. We report long-term outcomes of a non-myeloablative allogeneic stem cell transplantation regimen consisting of total skin electron beam therapy, total lymphoid irradiation and antithymocyte globulin. Our prospective cohort consisted of 41 patients with a higher proportion of MF (34MF, 7SS). Acute GVHD Grade 2 to 4 was seen in 31.7% and chronic GVHD Grade 2 to 4 in 24%. The cumulative incidence of non-relapse mortality was 9.8% at 1 year and 12.6% at 2 years. At Day +90 post-transplant 66% of patients had a complete response (CR). With a median post-transplant follow up of 5.27 years, the 5-year overall survival rate was 37.7% (MF 36.7%, SS 57.1%). The 5-year cumulative incidence of progressive disease or relapse was 52.7% in all patients but only 20.8% in those with CR at transplant compared to 70.6% in those not in CR at transplant (p = 0.006). Long term survival is possible in advanced MF and SS with non-myeloablative transplantation and outcomes are improved in patients with CR at transplant.
Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking
Authors: Chio Un Seng , Palovcak Eugene , Smith Anton A. A. , Autzen Henriette , Muñoz Elise N. , Yu Zanlin , Wang Feng , Agard David A. , Armache Jean-Paul , Narlikar Geeta J. , Cheng Yifan 
Published: March 2024

AbstractSingle-particle cryo-EM is widely used to determine enzyme-nucleosome complex structures. However, cryo-EM sample preparation remains challenging and inconsistent due to complex denaturation at the air-water interface (AWI). Here, to address this issue, we develop graphene-oxide-coated EM grids functionalized with either single-stranded DNA (ssDNA) or thiol-poly(acrylic acid-co-styrene) (TAASTY) co-polymer. These grids protect complexes between the chromatin remodeler SNF2h and nucleosomes from the AWI and facilitate collection of high-quality micrographs of intact SNF2h-nucleosome complexes in the absence of crosslinking. The data yields maps ranging from 2.3 to 3 Å in resolution. 3D variability analysis reveals nucleotide-state linked conformational changes in SNF2h bound to a nucleosome. In addition, the analysis provides structural evidence for asymmetric coordination between two SNF2h protomers acting on the same nucleosome. We envision these grids will enable similar detailed structural analyses for other enzyme-nucleosome complexes and possibly other protein-nucleic acid complexes in general.
Systematic review of the uncertainty of coral reef futures under climate change
Authors: Klein Shannon G. , Roch Cassandra , Duarte Carlos M. 
Published: March 2024

AbstractClimate change impact syntheses, such as those by the Intergovernmental Panel on Climate Change, consistently assert that limiting global warming to 1.5 °C is unlikely to safeguard most of the world’s coral reefs. This prognosis is primarily based on a small subset of available models that apply similar ‘excess heat’ threshold methodologies. Our systematic review of 79 articles projecting coral reef responses to climate change revealed five main methods. ‘Excess heat’ models constituted one third (32%) of all studies but attracted a disproportionate share (68%) of citations in the field. Most methods relied on deterministic cause-and-effect rules rather than probabilistic relationships, impeding the field’s ability to estimate uncertainty. To synthesize the available projections, we aimed to identify models with comparable outputs. However, divergent choices in model outputs and scenarios limited the analysis to a fraction of available studies. We found substantial discrepancies in the projected impacts, indicating that the subset of articles serving as a basis for climate change syntheses may project more severe consequences than other studies and methodologies. Drawing on insights from other fields, we propose methods to incorporate uncertainty into deterministic modeling approaches and propose a multi-model ensemble approach to generating probabilistic projections for coral reef futures.
Synapsin 2a tetramerisation selectively controls the presynaptic nanoscale organisation of reserve synaptic vesicles
Authors: Longfield Shanley F. , Gormal Rachel S. , Feller Matis , Parutto Pierre , Reingruber Jürgen , Wallis Tristan P. , Joensuu Merja , Augustine George J. , Martínez-Mármol Ramón , Holcman David , Meunier Frédéric A. 
Published: March 2024

AbstractNeurotransmitter release relies on the regulated fusion of synaptic vesicles (SVs) that are tightly packed within the presynaptic bouton of neurons. The mechanism by which SVs are clustered at the presynapse, while preserving their ability to dynamically recycle to support neuronal communication, remains unknown. Synapsin 2a (Syn2a) tetramerization has been suggested as a potential clustering mechanism. Here, we used Dual-pulse sub-diffractional Tracking of Internalised Molecules (DsdTIM) to simultaneously track single SVs from the recycling and the reserve pools, in live hippocampal neurons. The reserve pool displays a lower presynaptic mobility compared to the recycling pool and is also present in the axons. Triple knockout of Synapsin 1-3 genes (SynTKO) increased the mobility of reserve pool SVs. Re-expression of wild-type Syn2a (Syn2aWT), but not the tetramerization-deficient mutant K337Q (Syn2aK337Q), fully rescued these effects. Single-particle tracking revealed that Syn2aK337QmEos3.1 exhibited altered activity-dependent presynaptic translocation and nanoclustering. Therefore, Syn2a tetramerization controls its own presynaptic nanoclustering and thereby contributes to the dynamic immobilisation of the SV reserve pool.
Identifying regulators of aberrant stem cell and differentiation activity in colorectal cancer using a dual endogenous reporter system
Authors: Spisak Sandor , Chen David , Likasitwatanakul Pornlada , Doan Paul , Li Zhixin , Bala Pratyusha , Vizkeleti Laura , Tisza Viktoria , De Silva Pushpamali , Giannakis Marios , Wolpin Brian , Qi Jun , Sethi Nilay S. 
Published: March 2024

AbstractAberrant stem cell-like activity and impaired differentiation are central to the development of colorectal cancer (CRC). To identify functional mediators of these key cellular programs, we engineer a dual endogenous reporter system by genome-editing the SOX9 and KRT20 loci of human CRC cell lines to express fluorescent reporters, broadcasting aberrant stem cell-like and differentiation activity, respectively. By applying a CRISPR screen targeting 78 epigenetic regulators with 542 sgRNAs to this platform, we identify factors that contribute to stem cell-like activity and differentiation in CRC. Perturbation single cell RNA sequencing (Perturb-seq) of validated hits nominate SMARCB1 of the BAF complex (also known as SWI/SNF) as a negative regulator of differentiation across an array of neoplastic colon models. SMARCB1 is a dependency and required for in vivo growth of human CRC models. These studies highlight the utility of biologically designed endogenous reporter platforms to uncover regulators with therapeutic potential.
Cobalt catalyzed practical hydroboration of terminal alkynes with time-dependent stereoselectivity
Authors: Wen Jinglan , Huang Yahao , Zhang Yu , Grützmacher Hansjörg , Hu Peng 
Published: March 2024

AbstractStereodefined vinylboron compounds are important organic synthons. The synthesis of E−1-vinylboron compounds typically involves the addition of a B-H bond to terminal alkynes. The selective generation of the thermodynamically unfavorable Z-isomers remains challenging, necessitating improved methods. Here, such a proficient and cost-effective catalytic system is introduced, comprising a cobalt salt and a readily accessible air-stable CNC pincer ligand. This system enables the transformation of terminal alkynes, even in the presence of bulky substituents, with excellent Z-selectivity. High turnover numbers (>1,600) and turnover frequencies (>132,000 h−1) are achieved at room temperature, and the reaction can be scaled up to 30 mmol smoothly. Kinetic studies reveal a formal second-order dependence on cobalt concentration. Mechanistic investigations indicate that the alkynes exhibit a higher affinity for the catalyst than the alkene products, resulting in exceptional Z-selective performance. Furthermore, a rare time-dependent stereoselectivity is observed, allowing for quantitative conversion of Z-vinylboronate esters to the E-isomers.