bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of a maximal exercise test on neurocognitive function
Authors: Covassin Tracey , Weiss Leigh , Powell John , Womack Christopher 
Published: January 2007

Objective:To examine the effects of a maximal exercise test on cognitive function in recreational athletes.Design:A repeated-measures design was used to compare baseline with post-cognitive function and fatigue symptoms after a maximal exercise test.Setting:Division 1 American Midwestern University, (Michigan State University, Michigan, USA).Participants:102 male and female recreational athletes.Intervention:Participants in the experimental group (n = 54) were asked to perform a maximal treadmill exercise test to maximal oxygen uptake (VO2max). Participants in the control group were asked to rest for 15 min.Main outcome measurements:All participants were administered a neuropsychological test battery called Immediate Post-Concussion Assessment and Cognitive Testing (ImPACT) before and after exercise to measure neurocognitive function and fatigue symptoms.Results:Results revealed a significant group (control, experimental)×time (baseline, post-test 1, post-test 2) interaction for verbal memory composite scores (p = 0.025). Specifically, verbal memory composite scores decreased in the experimental group from baseline to post-test 1 (p = 0.00). These values returned to baseline 3 days after the Vo2max test (p = 0.00). Further analysis on verbal memory composite scores demonstrated significant differences on immediate recall memory (p = 0.00) and delayed recall memory (p = 0.00). No significant differences were observed for visual memory (p = 0.54), motor processing speed (p = 0.68) and reaction time (p = 0.44) composite scores between the experimental and control groups.Conclusion:The results of this study suggest that a maximal exercise test attenuated a limiting effect on cognitive function. When utilising a neuropsychological test battery to evaluate a patient who has sustained a head injury, the test should not be administered immediately after a practice or a game session.
Relationship between landing strategy and patellar tendinopathy in volleyball
Authors: Bisseling Rob W , Hof At L , Bredeweg Steef W , Zwerver Johannes , Mulder Theo 
Published: January 2007

Objective:The aetiology of patellar tendinopathy (jumper’s knee) remains unclear. To see whether landing strategy might be a risk factor for the development of this injury, this study examined whether landing dynamics from drop jumps differed among healthy volleyball players (CON) and volleyball players with a jumper’s knee. The patients with jumper’s knee were divided into an asymptomatic group with a previous jumper’s knee (PJK) and a symptomatic group with a recent jumper’s knee (RJK).Methods:Inverse dynamics analyses were used to estimate lower extremity joint dynamics from 30, 50 and 70 cm drop jumps in the three groups (CON, n = 8; PJK, n = 7; RJK, n = 9). A univariate repeated measures analysis of variance was used to compare the different landing techniques.Results:Data analysis of the landing dynamics revealed that PJK showed higher knee angular velocities (p<0.01), and higher ankle plantar flexion moment loading rate (p<0.01). Furthermore, strong tendencies of higher loading rate of vertical ground reaction force (p = 0.05) and higher knee extensor moment loading rate (p = 0.08) were found compared with CON. Higher values for peak knee moment, peak knee power and knee work (all p<0.01) were found for CON compared with RJK. The comparison of the two jumper’s knee groups yielded higher knee angular velocities (p<0.01), together with higher ankle plantar flexion and knee extensor moment loading rate (p<0.01 and p<0.05, respectively).Conclusion:Where RJK used a landing technique to avoid high patellar tendon loading, PJK used a stiffer landing strategy, which may be a risk factor in the development of patellar tendinopathy.
Microwave hyperthermia treatment increases heat shock proteins in human skeletal muscle
Authors: Ogura Yuji , Naito Hisashi , Tsurukawa Toshihiro , Ichinoseki-Sekine Noriko , Saga Norio , Sugiura Takao , Katamoto Shizuo 
Published: January 2007

Objective:To test the hypothesis that microwave hyperthermia treatment (MHT) increases heat shock proteins (HSPs) in the human vastus lateralis muscle.Methods:Four untrained healthy male volunteers participated in this study. The lateral side of the thigh of one leg (heated leg) was heated with a microwave generator (2.5 GHz, 150 W) for 20 min. At 1 day after the MHT, a muscle sample was taken from the heated leg. A control sample was taken from the unheated leg on another day of the MHT. For both legs, HSP90, HSP72 and HSP27 levels were compared.Results:The HSP90, HSP72 and HSP27 levels in heated legs were significantly higher than those in control legs (p<0.05).Conclusions:Application of MHT can increase the levels of several HSPs in human skeletal muscle.
The lowest surviving birth weight reported in a systemic lupus erythematosus patient: a review of the literature
Authors: Spiegel J , Ishimori M L , Wallace D J , Weisman M H 
Published: January 2007

The success rates for pregnancies in women with systemic lupus erythematosus(SLE) have improved over the years: however, pregnancy for women with active, serious, organ-threatening lupus continues to be a challenge. Preeclampsia and HELLP (hemolysis, elevated liver enzymes and low platelets) syndrome may complicate pregnancy especially in the setting of advanced maternal age, underlying SLE and chronic renal disease. We report the pregnancy course and outcome for a 35-year old woman with active lupus nephritis at the time of conception who developed severe preeclampsia and HELLP syndrome. The infant was delivered at 26-5/7 weeks gestation, which, associated with intrauterine growth retardation, led to a birth weight of only 470 g. We have reviewed the relevant literature for similar cases of prematurity, very low birth weight, and preeclampsia in the setting of underlying lupus in Medline between 1986 and 2006. This report represents the lowest birth weight pregnancy survival in a lupus patient and the first case of a survival in the second trimester with preeclampsia and HELLP syndrome.
Catastrophic antiphospholipid syndrome associated with malignancies (case report and review of the literature)
Authors: Ideguchi H , Ohno S , Ueda A , Ishigatsubo Y 
Published: January 2007

We describe a 58-year old female patient with rapid development of arterial and venous thromboembolisms, including deep vein thrombosis (DVT) in the lower limbs, recurrent cerebral infarctions and bilateral pulmonary emboli. Her laboratory data on admission showed positive anticardiolipin antibody of IgG isotype (IgG aCL) and positive anti-β2 glycoprotein-I antibody of IgG isotype (IgG aβ2-GPI), and decreased protein C activity and protein S antigen. Systemic examinations revealed the presence of an ovarian cancer. Surgical resection was attempted, but her cancer infiltrated the pelvic wall and could not be resected. Despite treatment with unfractionated heparin followed by warfarin, she died due to recurrent episodes of cerebral infarction. This case was considered as probable catastrophic antiphospholipid syndrome (CAPS), which might be associated with ovarian cancer. Known as Trousseau's syndrome, arterial and, more commonly, venous thrombosis is a frequent complication of cancer and sometimes a harbinger of occult cancer. Our case indicates that there is an overlap between antiphospholipid syndrome (APS) and Trousseau's syndrome. It is important to bear in mind that a thrombotic event associated with cancer can be the first manifestation of CAPS.
Agate and chalcedony from igneous and sedimentary hosts aged from 13 to 3480 Ma: a cathodoluminescence study
Authors: Moxon T. , Reed S. J. B. 
Published: January 2007

AbstractChalcedony and agates from a variety of world-wide hosts have been examined using cathodoluminescence (CL). Gaussian fitting of the experimental data shows that there are two dominant spectral emissions at ∼400 and ∼660 nm. A third subordinate peak is also found at ∼470, ∼560 or ∼620 nm. An age-related link is shown between the respective decreasing and increasing relative intensities of the 660 and 620 nm emissions. It is proposed that this change is due to a condensation reaction between neighbouring Si–OH groups eliminating water and forming a strained Si-O-Si bond.Agates from a variety of hosts and regions produced no clear demonstrable CL distinctions. However, a set of Western Australian agates was examined from host rocks that had been subjected to burial metamorphism. Cathodoluminescence produced different spectral emissions in the petrographic fibrous and granular regions of these agates. One agate shows a partial transformation of the petrographic fibrosity into granularity. This conversion is characterized by emission bands at 570 nm and 460 nm. Similar emission-band changes were produced by heating Brazilian agates for 35 days at 300°C. The identification of these changes in agate could serve as an indicator of palaeoheating within the parent rock.
Tracking magmatic processes through Zr/Hf ratios in rocks and Hf and Ti zoning in zircons: An example from the Spirit Mountain batholith, Nevada
Authors: Lowery Claiborne L. , Miller C. F. , Walker B. A. , Wooden J. L. , Mazdab F. K. , Bea F. 
Published: January 2007

AbstractZirconium and Hf are nearly identical geochemically, and therefore most of the crust maintains near-chondritic Zr/Hf ratios of ∼35–40. By contrast, many high-silica rhyolites and granites have anomalously low Zr/Hf (15–30). As zircon is the primary reservoir for both Zr and Hf and preferentially incorporates Zr, crystallization of zircon controls Zr/Hf, imprinting low Zr/Hf on coexisting melt. Thus, low Zr/Hf is a unique fingerprint of effective magmatic fractionation in the crust. Age and compositional zonation in zircons themselves provide a record of the thermal and compositional histories of magmatic systems. High Hf (low Zr/Hf) in zircon zones demonstrates growth from fractionated melt, and Ti provides an estimate of temperature of crystallization (TTiZ) (Watson and Harrison, 2005). Whole-rock Zr/Hf and zircon zonation in the Spirit Mountain batholith, Nevada, document repeated fractionation and thermal fluctuations. Ratios of Zr/Hf are ∼307–40 for cumulates and 18–30 for high-SiO2 granites. In zircons, Hf (and U) are inversely correlated with Ti, and concentrations indicate large fluctuations in melt composition and TTiZ (>100°C) for individual zircons. Such variations are consistent with field relations and ion-probe zircon geochronology that indicate a >1 million year history of repeated replenishment, fractionation, and extraction of melt from crystal mush to form the low Zr/Hf high-SiO2 zone.
Petrology and geodynamic significance of deerite-bearing metaquartzites from the Escambray Massif, Cuba
Authors: Grevel C. , Maresch W. V. , Stanek K.-P. , Grafe F. , Hoernes S. 
Published: January 2007

AbstractDeerite, a typical mineral of Fe-rich metacherts metamorphosed under blueschist conditions, is not rare, but known occurrences have up to now been restricted mainly to the Tethyan collisional zone and the Western Cordillera of North America. We describe a first occurrence in the high-pressure nappes of the Escambray Massif, Cuba, in the assemblage deerite + Mg-Al-poor riebeckite + magnetite + quartz ± garnet ± phengite ± aegirine. This assemblage typically forms during exhumation and accompanies late, stress-free annealing of the quartz matrix. Mg-Al-poor riebeckite overgrows older, large, oriented crystals of glaucophane, ferroglaucophane and Mg-Al-rich riebeckite (‘crossite’) during deerite formation. Early-formed hematite was largely replaced by magnetite. Deerite is very close to ideal composition, attaining >99% Si12O40(OH)5, allowing direct application of the experimentally determined P-T-fO2 stability field (Lattard and Le Breton, 1994). In combination with oxygen-isotope thermometry on magnetite-quartz, the crystallization conditions of the deerite-bearing assemblage can be constrained to ∼470°C, >15 kbar, and an oxygen fugacity restricted closely to the quartz-fayalite-magnetite buffer (fO2 ≈ 10−23 bar). Thus, the late-stage P-T path ofthe metacherts mirrors a steep P-T gradient of 10°C/km or less, requiring subduction ofthis part ofthe Antillean Island Arc to be still active during exhumation of the Escambray nappes.
Crystal-structure refinement of a Zn-rich kupletskite from Mont Saint-Hilaire, Quebec, with contributions to the geochemistry of zinc in peralkaline environments
Authors: Piilonen P. C. , Pekov I. V. , Back M. , Steede T. , Gault R. A. 
Published: January 2007

AbstractThe chemistry and crystal structure of a unique Zn-rich kupletskite: (K1.55Na0 .21Rb0.09Sr0.01)Σ1.86(Na0.82Ca0.18)Σ1.00(Mn4.72Zn1.66Na0.41Mg0.12)Σ7.00(Ti1.85Nb0.11Hf0.03)Σ1.99(Si7.99Al0.12)Σ8.11O26(OH)4(F0.77OH0.23)Σ1.00, from analkalin e pegmatite at Mont Saint-Hilaire, Quebec, Canada has been determined. Zn-rich kupletskite is triclinic,,a= 5.3765(4),b= 11.8893(11),c= 11.6997(10), α = 113.070(3), β = 94.775(2), γ = 103.089(3),R1 = 0.0570 for 3757 observed reflections withFo> 4σ(Fo). From the single-crystal X-ray diffraction refinement, it is clear that Zn2+shows a preference for the smaller,trans M(4) site (69%), yet is distributed amongst all three octahedral sites coordinated by 4 O2−and 2 OH−[M(2) 58% andM(3) 60%]. Of note is the lack of Zn inM(1), the larger and least-distorted of the four crystallographic sites, with an asymmetric anionic arrangement of 5 O2−and 1 OH−. The preference of Zn for octahedral sites coordinated by mixed ligands (O and OH) is characteristic of its behaviour in alkaline systems, in contrast to granitic systems where Zn tends to favour [4]-coordinated, OH− and H2O-free sites with only one ligand species (O, S, Cl, B, I). In alkaline systems,[4]Zn is only present in early sphalerite or in late-stage zeolite-like minerals. The bulk of Zn in alkaline systems is present as discrete[6]Zn phases such as members of the astrophyllite, labuntsovite, milarite and nordite groups, a result of the formation of network-formingcomplexes inthe low-temperature, low-fS2, high-alkalinity and highly oxidizing systems.
Low-T formation of high-Cr spinel with apparently primary chemical characteristics within podiform chromitite from Rayat, northeastern Iraq
Authors: Arai S. , Shimizu Y. , Ismail S. A. , Ahmed A. H. 
Published: January 2007

AbstractChemical modification of chromian spinel at low-T alteration was examined in detail for a podiform chromitite from a Tethyan ophiolitic mélange belt at Rayat, northeastern Iraq. The chromitite is highly brecciated and the matrix has been completely altered, producing chlorite and carbonate (dolomite and calcite). High-Cr, low-Fe3+ spinel has formed along the margins and cracks of chromian spinel grains throughout the alteration, associated with unaltered primary spinel and magnetite without ferritchromite. In associated harzburgites, only ferritchromite is found instead of the high-Cr, low-Fe3+ spinel. The high-Cr, low-Fe3+ secondary spinel apparently has chemical properties of mantle origin, plotted at the extension of ordinary mantle spinels on compositional spaces. The character is due to subtraction of Al as chlorite with the addition of an amount of magnetite component from the silicate matrix, which is small in volume relative to peridotite and composed of highly magnesian olivine (up to Fo97). We should treat high-Cr chromian spinels with caution in highly altered mantle-derived rocks, especially chromitite and other rocks with highly magnesian olivine, as well as in detrital particles for provenance study.
High glucose upregulates connective tissue growth factor expression in human vascular smooth muscle cells
Authors: Liu Xiaojing , Luo Fengming , Pan Kejian , Wu Wenchao , Chen Huaiqing 
Published: January 2007

Abstract

Background
Connective tissue growth factor (CTGF) is a potent profibrotic factor, which is implicated in fibroblast proliferation, angiogenesis and extracellular matrix (ECM) synthesis. It is a downstream mediator of some of the effects of transforming growth factor β (TGFβ) and is potentially induced by hyperglycemia in human renal mesangial cells. However, whether high glucose could induce the CTGF expression in vascular smooth muscle cells (VSMCs) remains unknown. Therefore, this study was designed to test whether high glucose could regulate CTGF expression in human VSMC. The effect of modulating CTGF expression on VSMC proliferation and migration was further investigated.

Results
Expression of CTGF mRNA was up-regulated as early as 6 hours in cultured human VSMCs after exposed to high glucose condition, followed by ECM components (collagen type I and fibronectin) accumulation. The upregulation of CTGF mRNA appears to be TGFβ-dependent since anti-TGFβ antibody blocks the effect of high glucose on CTGF gene expression. A small interference RNA (siRNA) targeting CTGF mRNA (CTGF-siRNA) effectively suppressed CTGF up-regulation stimulated by high glucose up to 79% inhibition. As a consequence of decreased expression of CTGF gene, the deposition of ECM proteins in the VSMC was also declined. Moreover, CTGF-siRNA expressing vector partially inhibited the high glucose-induced VSMC proliferation and migration.

Conclusion
Our data suggest that in the development of macrovascular complications in diabetes, CTGF might be an important factor involved in the patho-physiological responses to high glucose in human VSMCs. In addition, the modulatory effects of CTGF-siRNA during this process suggest that specific targeting CTGF by RNA interference could be useful in preventing intimal hyperplasia in diabetic macrovascular complications.
A strong constitutive ethylene-response phenotype conferred on Arabidopsis plants containing null mutations in the ethylene receptors ETR1 and ERS1
Authors: Qu Xiang , Hall Brenda P , Gao Zhiyong , Schaller G Eric 
Published: January 2007

Abstract

Background
The ethylene receptor family of Arabidopsis consists of five members, falling into two subfamilies. Subfamily 1 is composed of ETR1 and ERS1, and subfamily 2 is composed of ETR2, ERS2, and EIN4. Although mutations have been isolated in the genes encoding all five family members, the only previous insertion allele of ERS1 (ers1-2) is a partial loss-of-function mutation based on our analysis. The purpose of this study was to determine the extent of signaling mediated by subfamily-1 ethylene receptors through isolation and characterization of null mutations.

Results
We isolated new T-DNA insertion alleles of subfamily 1 members ERS1 and ETR1 (ers1-3 and etr1-9, respectively), both of which are null mutations based on molecular, biochemical, and genetic analyses. Single mutants show an ethylene response similar to wild type, although both mutants are slightly hypersensitive to ethylene. Double mutants of ers1-3 with etr1-9, as well as with the previously isolated etr1-7, display a constitutive ethylene-response phenotype more pronounced than that observed with any previously characterized combination of ethylene receptor mutations. Dark-grown etr1-9;ers1-3 and etr1-7;ers1-3 seedlings display a constitutive triple-response phenotype. Light-grown etr1-9;ers1-3 and etr1-7;ers1-3 plants are dwarfed, largely sterile, exhibit premature leaf senescence, and develop novel filamentous structures at the base of the flower. A reduced level of ethylene response was still uncovered in the double mutants, indicating that subfamily 2 receptors can independently contribute to signaling, with evidence suggesting that this is due to their interaction with the Raf-like kinase CTR1.

Conclusion
Our results are consistent with the ethylene receptors acting as redundant negative regulators of ethylene signaling, but with subfamily 1 receptors playing the predominant role. Loss of a single member of subfamily 1 is largely compensated for by the activity of the other member, but loss of both subfamily members results in a strong constitutive ethylene-response phenotype. The role of subfamily 1 members is greater than previously suspected and analysis of the double mutant null for both ETR1 and ERS1 uncovers novel roles for the receptors not previously characterized.
Production of recombinant antibody fragments in Bacillus megaterium
Authors: Jordan Eva , Hust Michael , Roth Andreas , Biedendieck Rebekka , Schirrmann Thomas , Jahn Dieter , Dübel Stefan 
Published: January 2007

Abstract

Background
Recombinant antibodies are essential reagents for research, diagnostics and therapy. The well established production host Escherichia coli relies on the secretion into the periplasmic space for antibody synthesis. Due to the outer membrane of Gram-negative bacteria, only a fraction of this material reaches the medium. Recently, the Gram-positive bacterium Bacillus megaterium was shown to efficiently secrete recombinant proteins into the growth medium. Here we evaluated B. megaterium for the recombinant production of antibody fragments.

Results
The lysozyme specific single chain Fv (scFv) fragment D1.3 was succesfully produced using B. megaterium. The impact of culture medium composition, gene expression time and culture temperatures on the production of functional scFv protein was systematically analyzed. A production and secretion at 41°C for 24 h using TB medium was optimal for this individual scFv. Interestingly, these parameters were very different to the optimal conditions for the expression of other proteins in B. megaterium. Per L culture supernatant, more than 400 μg of recombinant His6-tagged antibody fragment were purified by one step affinity chromatography. The material produced by B. megaterium showed an increased specific activity compared to material produced in E. coli.

Conclusion
High yields of functional scFv antibody fragments can be produced and secreted into the culture medium by B. megaterium, making this production system a reasonable alternative to E. coli.
SCF Fbx4/alphaB-crystallin cyclin D1 ubiquitin ligase: a license to destroy
Authors: Barbash Olena , Lin Douglas I , Diehl J Alan 
Published: January 2007

Abstract
Cyclin D1 is an allosteric regulator for cyclin-dependent kinases 4 and 6 (CDK4/6). The cyclin D/CDK4 kinase promotes G1/S transition through the posttranslational modification and the subsequent inactivation of the retinoblastoma (Rb) protein and related family members (p107 and p130). Accumulation of cyclin D1 is tightly regulated through various mechanisms including transcription, protein localization and ubiquitin-dependent proteolysis. While regulators of cyclin D1 gene expression have been under considerable scrutiny, the identity of the protein complex that targets cyclin D1 protein for degradation, the putative E3 ubiquitin ligase, has remained obscure. In a recent report, Lin et al [1] describe the identification and characterization of a novel SCF, wherein FBX4 and αB-crystallin serve as specificity factors that direct ubiquitination of phosphorylated cyclin D1. As cyclin D1 overexpression in human cancer has been postulated to occur through the loss of degradation machinery, the identification of the SCFFbx4/αB-crystallin ligase will allow new experimental approaches that address mechanisms of cyclin D1 overexpression in human cancer.
An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta
Authors: Wang John , Jemielity Stephanie , Uva Paolo , Wurm Yannick , Gräff Johannes , Keller Laurent 
Published: January 2007

AbstractAnts display a range of fascinating behaviors, a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits: a tentatively annotated expressed sequence tag (EST) resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21,715 ESTs, which represent 11,864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray.