bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissociable neural effects of stimulus valence and preceding context during the inhibition of responses to emotional faces
Authors: Schulz Kurt P. , Clerkin Suzanne M. , Halperin Jeffrey M. , Newcorn Jeffrey H. , Tang Cheuk Y. , Fan Jin 
Published: December 2008

AbstractSocially appropriate behavior requires the concurrent inhibition of actions that are inappropriate in the context. This self‐regulatory function requires an interaction of inhibitory and emotional processes that recruits brain regions beyond those engaged by either processes alone. In this study, we isolated brain activity associated with response inhibition and emotional processing in 24 healthy adults using event‐related functional magnetic resonance imaging (fMRI) and a go/no‐go task that independently manipulated the context preceding no‐go trials (ie, number of go trials) and the valence (ie, happy, sad, and neutral) of the face stimuli used as trial cues. Parallel quadratic trends were seen in correct inhibitions on no‐go trials preceded by increasing numbers of go trials and associated activation for correct no‐go trials in inferior frontal gyrus pars opercularis, pars triangularis, and pars orbitalis, temporoparietal junction, superior parietal lobule, and temporal sensory association cortices. Conversely, the comparison of happy versus neutral faces and sad versus neutral faces revealed valence‐dependent activation in the amygdala, anterior insula cortex, and posterior midcingulate cortex. Further, an interaction between inhibition and emotion was seen in valence‐dependent variations in the quadratic trend in no‐go activation in the right inferior frontal gyrus and left posterior insula cortex. These results suggest that the inhibition of response to emotional cues involves the interaction of partly dissociable limbic and frontoparietal networks that encode emotional cues and use these cues to exert inhibitory control over the motor, attention, and sensory functions needed to perform the task, respectively. Hum Brain Mapp, 2009. © 2008 Wiley‐Liss, Inc.
Ethanol metabolism alters major histocompatibility complex class I–restricted antigen presentation in liver cells #
Authors: Osna Natalia A. , White Ronda L. , Thiele Geoffrey M. , Donohue Terrence M. 
Published: December 2008

Abstract
The proteasome is a major enzyme that cleaves proteins for antigen presentation. Cleaved peptides traffic to the cell surface, where they are presented in the context of major histocompatibility complex (MHC) class I. Recognition of these complexes by cytotoxic T lymphocytes is crucial for elimination of cells bearing “nonself” proteins. Our previous studies revealed that ethanol suppresses proteasome function in ethanol-metabolizing liver cells. We hypothesized that proteasome suppression reduces the hydrolysis of antigenic peptides, thereby decreasing the presentation of the peptide MHC class I complexes on the cell surface. To test this we used the mouse hepatocyte cell line (CYP2E1/ADH-transfected HepB5 cells) or primary mouse hepatocytes, both derived from livers of C57Bl/6 mice, which present the ovalbumin peptide, SIINFEKL, complexed with H2Kb. To induce H2Kb expression, HepB5 cells were treated with interferon gamma (IFNγ) and then exposed to ethanol. In these cells, ethanol metabolism decreased not only proteasome activity, but also hydrolysis of the C-extended peptide, SIINFEKL-TE, and the presentation of SIINFEKL-H2Kb complexes measured after the delivery of SIINFEKL-TE to cytoplasm. The suppressive effects of ethanol were, in part, attributed to ethanol-elicited impairment of IFNγ signaling. However, in primary hepatocytes, even in the absence of IFNγ, we observed a similar decline in proteasome activity and antigen presentation after ethanol exposure. Conclusion: Proteasome function is directly suppressed by ethanol metabolism and indirectly by preventing the activating effects of IFNγ. Ethanol-elicited reduction in proteasome activity contributes to the suppression of SIINFEKL-H2Kb presentation on the surface of liver cells. (Hepatology 2009.)
An integral system‐ergonomic approach for IT‐based process management in complex work environments by example of manufacturing and health care
Authors: Friesdorf Florian , Plavšić Marina , Bubb Heiner 
Published: December 2008

AbstractRecent trends in manufacturing and health care move these two work systems closer together from a system ergonomics point of view. Individual treatment of products, especially patients, by specialists in a distributed environment demand information technology (IT)‐based support suitable for complex systems. IT‐based support of processes in complex systems is difficult due to the lack of standard processes. IT support also means to rethink processes to use efficiency potentials. Close cooperation of users and software developers is needed to increase the ergonomic quality of the system. Therefore, suitable tools are needed: UML is available as the standard industry modeling language, Zope/Plone as the quasi‐standard for content management systems, SimPy as an object‐oriented simulation tool for event‐triggered processes, and ACT‐R as a powerful cognitive architecture for simulation of human information processes. The integration of these tools enables system‐ergonomic support of processes in the complex work system as well as of the development and deployment process. It is the base of an integral system‐ergonomic approach for IT‐based process management. Knowledge gained during process analysis either enters models or leads to the extension and adaptation of the tool chain. The models serve as basis for discussion among system ergonomists, programmers, and specialists from the work system. Further, they are understood by simulation and process support tools. Transcoding efforts between humans with different professional backgrounds and machines are reduced, and the flexibility demanded by complex systems is met. © 2008 Wiley Periodicals, Inc.
Identification of Fat4 as a candidate tumor suppressor gene in breast cancers
Authors: Qi Chao , Zhu Yiwei Tony , Hu Liping , Zhu Yi‐Jun 
Published: December 2008

AbstractFat, a candidate tumor suppressor in Drosophila, is a component of Hippo signaling pathway involved in controlling organ size. We found that a ∼3 Mbp deletion in mouse chromosome 3 caused tumorigenesis of a non‐tumorigenic mammary epithelial cell line. The expression of Fat4 gene, one member of the Fat family, in the deleted region was inactivated, which resulted from promoter methylation of another Fat4 allele following the deletion of one Fat4 allele. Re‐expression of Fat4 in Fat4‐deficient tumor cells suppressed the tumorigenecity whereas suppression of Fat4 expression in the non‐tumorigenic mammary epithelial cell line induced tumorigenesis. We also found that Fat4 expression was lost in a large fraction of human breast tumor cell lines and primary tumors. Loss of Fat4 expression in breast tumors was associated with human Fat4 promoter methylation. Together, these findings suggest that Fat4 is a strong candidate for a breast tumor suppressor gene. © 2008 Wiley‐Liss, Inc.
Identification of CCDC62‐2 as a novel cancer/testis antigen and its immunogenicity
Authors: Domae Shohei , Nakamura Yoichi , Nakamura Yurika , Uenaka Akiko , Wada Hisashi , Nakata Masao , Oka Mikio , Kishimoto Koji , Tsukamoto Goichi , Yoshihama Yasuto , Matsuoka Junji , Gochi Akira , Kohno Shigeru , Saika Takashi , Sasaki Akira , Nakayama Eiichi , Ono Toshiro 
Published: December 2008

AbstractCancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis‐specific expression profiles in the Unigene database, including coiled‐coil domain containing 62 (CCDC62). RT‐PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62‐2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62‐2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62‐2 was confirmed by western blot. CCDC62‐2 is a CT antigen that is immunogenic in cancer patients. © 2008 Wiley‐Liss, Inc.
Nitroimidazole derivatives: non‐randomness sister chromatid exchanges in human peripheral blood lymphocytes
Authors: Ana Carballo Marta , Martinez Romari Alejandra , Mudry Marta Dolores 
Published: December 2008

AbstractNitroimidazole derivatives exhibited genotoxic effect in different experimental conditions. This study focuses on an evaluation of possible genomic targets, at a chromosomal level, of two 5‐nitroimidazoles (ornidazole and metronidazole) using thein vitrohuman peripheral blood culture as experimental system. We observed that both derivatives showed a decrease in mitotic index (MI) (P< 0.001), an increase in sister chromatid exchanges (SCE) frequency (P< 0.001) and no modifications in cellular proliferation kinetics (CPK). As a null hypothesis we considered the assumption that larger chromosomes should harbor more SCE, which was viewed using a novel sequential G‐band (400 band resolution)/SCE technique. The analysis showed highly significant chi square values (P< 0.001), indicating that SCE frequency per chromosome is not proportional to chromosome length. SCE could be considered an instability indicator due to the high correlation between SCEs in certain chromosomal bands and the exposure to nitroimidazole derivatives. Copyright © 2008 John Wiley & Sons, Ltd.
A consensus line search algorithm for molecular potential energy functions
Authors: Rurainski Alexander , Hildebrandt Andreas , Lenhof Hans‐Peter 
Published: December 2008

AbstractForce field based energy minimization of molecular structures is a central task in computational chemistry and biology. Solving this problem usually requires efficient local minimization techniques, i.e., iterative two‐step methods that search first for a descent direction and then try to estimate the step width. The second step, the so called line search, typically uses polynomial interpolation schemes to estimate the next trial step. However, dependent on local properties of the objective function alternative schemes may be more appropriate especially if the objective function shows singularities or exponential behavior. As the choice of the best interpolation scheme cannot be made a priori, we propose a new consensus line search approach that performs several different interpolation schemes at each step and then decides which one is the most reliable at the current position. Although a naive consensus approach would lead to severe performance impacts, our method does not require additional evaluations of the energy function, imposing only negligible computational overhead. Additionally, our method can be easily adapted to the local behavior of other objective functions by incorporating suitable interpolation schemes or omitting non‐fitting schemes. The performance of our consensus line search approach has been evaluated and compared to established standard line search algorithms by minimizing the structures of a large set of molecules using different force fields. The proposed algorithm shows better performance in almost all test cases, i.e., it reduces the number of iterations and function and gradient evaluations, leading to significantly reduced run times. © 2008 Wiley Periodicals, Inc. J Comput Chem, 2009
TGF‐β promotes cell death and suppresses lactation during the second stage of mammary involution
Authors: Bierie Brian , Gorska Agnieszka E. , Stover Daniel G. , Moses Harold L. 
Published: December 2008

AbstractTransforming growth factor beta (TGF‐β) ligands are known to regulate virgin mammary development and contribute to initiation of post‐lactation involution. However, the role for TGF‐β during the second phase of mammary involution has not been addressed. Previously, we have used an MMTV‐Cre transgene to delete exon 2 from the Tgfbr2 gene in mammary epithelium, however we observed a gradual loss of TβRII deficient epithelial cells that precluded an accurate study of the role for TGF‐β signaling during involution timepoints. Therefore, in order to determine the role for TGF‐β during the second phase of mammary involution we have now targeted TβRII ablation within mammary epithelium using the WAP‐Cre transgene [TβRII(WKO)Rosa26R]. Our results demonstrated that TGF‐β regulates commitment to cell death during the second phase of mammary involution. Importantly, at day 3 of mammary involution the Na–Pi type IIb co‐transporter (Npt2b), a selective marker for active lactation in luminal lobular alveolar epithelium, was completely silenced in the WAP‐Cre control and TβRII(WKO)Rosa26R tissues. However, by day 7 of involution the TβRII(WKO)Rosa26R tissues had distended lobular alveoli and regained a robust Npt2b signal that was detected at the apical luminal surface. The Npt2b abundance and localization positively correlated with elevated WAP mRNA expression, suggesting that the distended alveoli were the result of an active lactation program rather than residual milk protein and lipid accumulation. In summary, the results suggest that an epithelial cell response to TGF‐β signaling regulates commitment to cell death and suppression of lactation during the second phase of mammary involution. J. Cell. Physiol. 219: 57–68, 2009. © 2008 Wiley‐Liss, Inc.
2‐(2‐Aminoethylamino)‐5‐nitropyridine as a basic matrix for negative‐mode matrix‐assisted laser desorption/ionization analysis of phospholipids
Authors: Lorkiewicz Pawel , Cecilia Yappert M. 
Published: December 2008

AbstractFast and easy analysis of phospholipids (PLs) by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) has been well demonstrated. However, when using common organic matrices, such as 2,5‐dihydroxybenzoic acid (DHB), the detection of most PL classes in positive‐ion mode is difficult when PLs containing zwitterionic groups, such as phosphatidylcholines (PCs) and sphingomyelins (SMs) are present. To reduce this limitation, 2‐(2‐aminoethyloamino)‐5‐nitropyridine (AAN), a basic compound, was evaluated as an alternative matrix. Negative‐ion spectra showed enhanced detection of phosphatidyl ethanolamines (PEs), phosphatidyl serines (PSs), phosphatidyl glycerols (PGs), and phosphatidyl inositols (PIs) in simple mixtures and in a crude methanolic soybean extract. The relative ionization efficiency (RIE) was highest for PIs and lowest for PGs, PSs, and PEs. Compared to DHB and para‐nitroaniline, AAN resulted in greater sensitivity for the detection of PL classes in the negative mode. Indeed, the S/N ratio was nearly an order of magnitude higher than that reported for similar PI concentrations but with DHB. MALDI spots produced with AAN were homogeneous thus allowing automation and improved reproducibility. Positive‐mode traces could also be acquired with AAN as the matrix, but with lower sensitivity than in the negative mode. Copyright © 2008 John Wiley & Sons, Ltd.
An analysis of present and future ECHAM5 pressure fields using a classification of circulation patterns
Authors: Demuzere M. , Werner M. , van Lipzig N. P. M. , Roeckner E. 
Published: December 2008

AbstractSeveral subjective and objective methods for the classification of circulation patterns into categories have been developed over the past century. In this study, we used the automated Lamb weather type (WT) classification method, based on mean sea level pressure (MSLP) to examine present and future circulation patterns above Western and Central Europe. First, the European Center for Medium‐Range Weather Forecast 40‐year reanalysis data (ECMWF‐ERA40) is used to evaluate the occurring WTs within the newly developed ECHAM5‐MPI/OM model for the period 1961–2000. Our analysis shows that the ECHAM5 model is capable of reproducing circulation patterns for the October to April season. For the remaining part of the year, there are some significant differences in eastern and western directional circulation types. Therefore, in the second part of this study, past, present and future ECHAM5 pressure fields are investigated for the autumn and winter season only. Finally, long‐term trends of MSLP fields of the A1B scenario simulation using ECHAM5‐MPI/OM for the period 1860–2100 show a significant increase in western circulation and anticyclonic WTs over Western and Central Europe, accompanied by a decrease in eastern circulation and cyclonic WTs. Copyright © 2008 Royal Meteorological Society
Nutritional and toxicological evaluation of wheat grown on soils with or without fly ash treatment
Authors: Kandlakunta Bhaskarachary , Punna Ramulu , Paruchuri Udayasekhararao , Sangras Bapurao , Krishnaswamy Kamala , Qadri Syed , Putcha Udayakumar , Boindala Sesikeran 
Published: December 2008

AbstractBACKGROUND: Fly ash disposal has recently posed a huge environmental problem due to storage, apart from apprehension about possible contamination of soil, crop produce and ground water with toxic trace and heavy metal and radio nuclides. The purpose of the present study was to evaluate nutritional and toxicological aspects of wheat grown on soils treated with fly ash. Wheat was grown on soils with fly ash (200 t ha−1) and without fly ash at two different geographical locations in India. One kilogram each of 36 samples randomly collected from three replicates of wheat grown with and without fly ash were subjected to various analytical techniques to determine the nutrient composition, mineral, trace element and heavy metal content. Furthermore, wheat grown on soils treated with fly ash was also incorporated into the diet at 90% level, fed as a feed for 26 weeks to Wistar/NIN rats for protein and toxicological evaluation.RESULTS: Moisture, protein and ash content of wheat samples showed no difference between fly ash treated and controls. Similar observations were also seen with trace and heavy elements.CONCLUSION: Results indicated there is no difference between wheat samples grown in soils with fly ash and without fly ash. Studies also clearly indicated that there are no adverse effects on haematological, biochemical or histopathological parameters when wheat was fed to rats for 6 months. This indicates that wheat grown on fly ash treated soils may be safe for human consumption. Copyright © 2008 Society of Chemical Industry
Atypical dystonic shoulder movements following neuralgic amyotrophy
Authors: Abdo William F. , Bloem Bastiaan R. , Eijk Jeroen J. , Geurts Alexander C. , van Alfen Nens , van de Warrenburg Bart P.C. 
Published: December 2008

AbstractPeripherally induced movement disorders are relatively rare. Here, we present 3 patients who suffered a lesion of the brachial plexus because of neuralgic amyotrophy and developed involuntary movements of their shoulder muscles. The nature of the involuntary movements, which did not easily comply with classic descriptions of hyperkinetic movement disorders, is probably best referred to as dystonia. © 2008 Movement Disorder Society
Long‐term outcome of early versus delayed rasagiline treatment in early Parkinson's disease
Authors: Hauser Robert A. , Lew Mark F. , Hurtig Howard I. , Ondo William G. , Wojcieszek Joanne , Fitzer‐Attas Cheryl J. ,   on behalf of the TEMPO Open‐label Study Group
Published: December 2008

AbstractThe purpose of this study to compare the long‐term clinical outcome of early versus delayed rasagiline treatment in early Parkinson's disease (PD). Subjects (N = 404) were randomly assigned to initial treatment with rasagiline (early‐start group) or placebo for 6 months followed by rasagiline (delayed‐start group) in the TEMPO study. Subjects who chose to participate in an open‐label extension (N = 306) continued to receive rasagiline as well as other PD medications as needed. Average (±SD) duration in the study was 3.6 ± 2.1 years; 177 subjects received rasagiline for ≥5.0 years. Over the entire 6.5‐year follow‐up period, the adjusted mean difference in change from baseline in total UPDRS scores was 2.5 units (SE 1.1; P = 0.021) or 16% (SE 5.7; P = 0.006) in favor of the early‐start versus delayed‐start rasagiline group. Although the interaction between treatment and time was significant, values for the early‐start group were better than the delayed‐start group across all time points. Significantly less worsening (percent change) in total UPDRS scores was observed in the early‐start group at the time points 0.5, 1.5, 2.0, 3.0, 4.5, 5.0, and 5.5 years (P < 0.05). Compared to delayed start, early initiation of rasagiline provided long‐term clinical benefit, even in the face of treatment with other dopaminergic agents. This might reflect enduring benefits due to neuroprotection or effects on compensatory mechanisms in early PD. © 2008 Movement Disorder Society
Differentiable structure of the set of coaxial stress–strain tensors
Authors: Clotet Josep , Magret M. Dolors , Peña Marta 
Published: December 2008

AbstractIn order to study stress–strain tensors, we consider their representations as pairs of symmetric 3 × 3‐matrices and the space of such pairs of matrices partitioned into equivalence classes corresponding to change of bases. We see that these equivalence classes are differentiable submanifolds; in fact, orbits under the action of a Lie group. We compute their dimension and obtain miniversal deformations. Finally, we prove that the space of coaxial stress–strain tensors is a finite union of differentiable submanifolds. Copyright © 2008 John Wiley & Sons, Ltd.
Prediction of water's isotropic nuclear shieldings and indirect nuclear spin–spin coupling constants (SSCCs) using correlation‐consistent and polarization‐consistent basis sets in the Kohn–Sham basis set limit
Authors: Kupka Teobald 
Published: December 2008

AbstractDensity functional theory (DFT) was used to estimate water's isotropic nuclear shieldings and indirect nuclear spin–spin coupling constants (SSCCs) in the Kohn–Sham (KS) complete basis set (CBS) limit. Correlation‐consistent cc‐pVxZ and cc‐pCVxZ (x = D, T, Q, 5, and 6), and their modified versions (ccJ‐pVxZ, unc‐ccJ‐pVxZ, and aug‐cc‐pVTZ‐J) and polarization‐consistent pc‐n and pcJ‐n (n = 0, 1, 2, 3, and 4) basis sets were used, and the results fitted with a simple mathematical formula. The performance of over 20 studied density functionals was assessed from comparison with the experiment. The agreement between the CBS DFT‐predicted isotropic shieldings, spin–spin values, and the experimental values was good and similar for the modified correlation‐consistent and polarization‐consistent basis sets. The BHandH method predicted the most accurate 1H, 17O isotropic shieldings and 1J(OH) coupling constant (deviations from experiment of about − 0.2 and − 1 ppm and 0.6 Hz, respectively). The performance of BHandH for predicting water isotropic shieldings and 1J(OH) is similar to the more advanced methods, second‐order polarization propagator approximation (SOPPA) and SOPPA(CCSD), in the basis set limit. Copyright © 2008 John Wiley & Sons, Ltd.