bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a Novel Prognostic Model for Low-grade Glioma Based on m6A-Associated Immune Genes and Identifying a New Biomarker
Authors: Zhang Yiling , Luo Na , Li Xiaoyu , zeng chuanfei , Zhu Wenjun , Fu Min , Yang Feng , Chen Ziqi , Zhang Qiang , Peng Bi , Chen Xin , Li Qianxia , Liu Yuanhui , Zhang Yuanyuan , Peng Xiaohong , Hu Guangyuan 
Published: March 2024

Abstract

Low-grade glioma (LGG) is a lower malignancy and slower-growing primary tumor of the nervous system. Methylation of N6-methyl adenosine (m6A) has important roles in the growth of tumors and cellular biological processes. The immune system is involved in tumourigenesis and development and plays a certain role in tumor therapy and resistance to drugs. There have been no in-depth studies on m6A-related immune markers in LGG. We obtained gene mutation data, gene expression, and related clinical information of LGG patients from the Chinese Glioma Genome Atlas (CGGA) database and the Cancer Genome Atlas (TCGA). Then, the prognostic model was calculated using multivariate Cox, LASSO, and univariate Cox analyses. A dynamic nomograph online app was also developed based on this model. In addition, for the screened model genes, we performed correlation analyses in the clinical staging, immunological subtype, and microenvironmental aspects. Finally, we determined the biological role of FBXO4 in glioma cells by quantitative reverse transcription-polymerase chain reaction, cell proliferation assay, and cell migration assay. Our prognostic models can accurately and efficiently help investigators analyze the prognosis of LGG patients. In addition, the correlation analysis between m6Ascore and tumor microenvironment can provide a basis for further exploration.
Development of a Biodegradable Polymer-based Implant to Release Dual Drugs for Post-operative Management of Cataract Surgery
Authors: Subhash Nayana E , Nair Soumya , Srini Srilatha Parampalli , Theruveethi Nagarajan , Bhandary Sulatha V , Guru Bharath Raja 
Published: March 2024

Abstract

Cataract surgery is followed by post-operative eye drops for a duration of 4-6 weeks. The multitude of ocular barriers, coupled with the discomfort experienced by both the patient and their relatives in frequently administering eye drops, significantly undermines patient compliance, ultimately impeding the recovery of the patient. This study aimed to design and develop an ocular drug delivery system as an effort to achieve a drop-free post-operative care after cataract surgery. An implant was prepared containing a biodegradable polymer Poly-lactic-co-glycolic acid (PLGA), Dexamethasone (DEX) as an anti-inflammatory agent, and Moxifloxacin(MOX) as an antibiotic. Implant characterization and drug loading analysis were conducted. In vitro drug release profile showed that the release of the two drugs are correlated with the clinical prescription for post operative eye drops. In vivo study was conducted on New Zealand albino rabbits where one eye underwent cataract surgery, and the drug delivery implant was inserted into the capsular bag after placement of the synthetic intraocular lens (IOL). Borderline increase in the intraocular pressure (IOP) was noted in the test sample group. Slit-lamp observations revealed no significant anterior chamber reaction in all study groups. Histopathology study of the operated eye revealed no significant pathology in the test samples. This work aims at developing the intra ocular drug delivery implant which will replace the post-operative eye drops and help the patient with the post-operative hassle of eye drops.
NELF coordinates Pol II transcription termination and DNA replication initiation
Authors: Nojima Takayuki , Nakayama Chihiro , Daigaku Yasukazu , Aoi Yuki , Fang Qi , Kimura Hiroshi , Shilatifard Ali , Tellier Michael 
Published: March 2024

Abstract
Regulation of RNA polymerase II (Pol II) transcription is closely associated with cell proliferation. However, it remains unclear how the Pol II transcription program is altered in cancer to favour cell growth. Here, we find that gene expression of NELFCD, a known negative elongation factor, is up-regulated in colorectal tumours. To dissect the direct role of NELF-C on Pol II transcription in such cancer, we employed an auxin-dependent protein degradation system for NELF-C in combination with nascent transcript sequencing technologies. Strikingly, we demonstrated that the acute loss of NELF-C protein globally perturbs Pol II transcription termination and also increases transcription elongation rate, independently of promoter-proximal Pol II pausing. This results in Pol II transcription into DNA replication initiation zones, and may link to failure of the cell cycle transition into S phase. We anticipate that NELF will be a potential therapeutic target to restrict colorectal cancers by promoting transcription-replication conflict.
Relationship between Mental Workload and Musculoskeletal Disorders and Intention to Leave Job among Nurses Working at Neonatal and Pediatric Departments: A Cross-sectional Study in Iran
Authors: Naserian Elham , Pouladi Shahnaz , Bagherzadeh Razieh , Ravanipour Maryam 
Published: March 2024

Abstract

BackgroundNurses’ intention to leave their jobs could be influenced by several factors, including mental workload, as one of the most important occupational stressors, and physical workload induced by working conditions. The present study aims to identify the relationship between mental workload and musculoskeletal disorders and intention to leave the job among nurses working at neonatal and pediatric departments.MethodsThis descriptive-analytical study was conducted on 145 nurses working at neonatal and pediatric departments in six hospitals in Bushehr Province using full-census method. The data were collected using national aeronautics and space administration-task load index (NASA-TLX), Cornell musculoskeletal discomfort questionnaire (CMDQ) and Mobley and Horner’s voluntary turnover questionnaire. The data were analyzed using descriptive statistics, independent t-test, Mann-Whitney U test, one-way analysis of variance (ANOVA), Kruskal-Wallis test, Pearson’s and Spearman correlation tests and hierarchical multivariate linear regression in simultaneous model in SPSS 19.0.ResultsThe mean score of intention to leave the job was 9.57 ± 3.20 (higher than the moderate level) and the mean mental workload was 71.65 ± 15.14 (high level). Pain in at least one of the legs (100%), back (77.3%) and knees (76.6%) was highly prevalent. However, no statistically significant correlation was found between musculoskeletal disorder categories and intention to leave the job (p > 0.05). The regression analysis results revealed among mental workload domains, only effort-induced workload was negatively and significantly correlated with intention to leave the job (p = 0.003; β=-0.078). However, the number of night shifts per month was positively and significantly correlated with intention to leave the job (p = 0.001; β = 0.176).ConclusionsPlanning for appropriate allocation of night shifts, investigating the etiology of musculoskeletal disorders and providing solutions for reducing mental workload should be prioritized by policymakers, while maintaining pediatric nurses’ motivation for making efforts.
Assessing Neuroprotective Effects of Mitoquinone on H2O2-Induced Neurotoxicity in SH-SY5Y Neuroblastoma Cells
Authors: Barsa Chloe , Hayar Berthe , Eid Ali , Goli Mona , Yehya Amani , Mechref Yehia , El-Khoury Riyad , Kheir Wassim Abou , Darwiche Nadine , Kobeissy Firas 
Published: March 2024

Abstract

Oxidative stress, a major player in secondary brain injury, has been shown to contribute to apoptosis, neuroinflammation, and mitochondrial dysfunction. The possibility of targeting the Nrf2-Keap-ARE pathway, using mitochondria-targeted antioxidants, such as mitoquinone (MitoQ), has been proposed to treat neurotoxicity. The neuroprotective effects of MitoQ on human neuroblastoma SH-SY5Y cells were assessed by MTT assay, SRB assay, and propidium iodide stain using MitoQ at concentrations of 0.03 and 0.05 µg/mL as pre-treatment or post-treatment for hydrogen peroxide (H2O2)-induced stress. Oxidative stress was evaluated by NBT assay and DHE staining while mitochondrial integrity was studied using MitotrackerGreen dye. The gene expression profile of the antioxidant genes Nrf2, SOD1, HOX1, and CAT and the inflammatory genes COX-2 and NFκB were investigated via RT-qPCR along with immunofluorescence imaging. Our results showed that pre-treatment with MitoQ protected SH-SY5Y cells by increasing cell viability, decreasing cell growth inhibition, preserving cell morphology and cell cycle integrity, and attenuating oxidative stress progression while preserving mitochondrial phenotype. The Nrf2-Keap-ARE pathway was demonstrated to be contributing to the protective effects of MitoQ with an upregulation of the antioxidant genes Nrf2 and HMOX1 along with the normalization of SOD1 gene expression. Finally, the decrease in COX-2 levels underscores the anti-inflammatory effect of the antioxidant which supports the use of MitoQ as a treatment for neurotoxicity.
Synthesis and Anti-Cancer Investigations of Novel Copper(II) Complexes Based on Adenine
Authors: Zhai Xiaoyan , Hanibah Hussein , Hashim Nor Zakiah Nor , Zhang Juzheng , Ma Xianli , Wei Lilan , Zhou Xiaoqun 
Published: March 2024

Abstract

Platinum-based chemotherapeutics have played a critical role in oncology for decades. However, their broader utility is hindered by the advent of severe side effects and the emergence of drug resistance. The pursuit of alternative agents, particularly non-platinum (non-Pt) metal complexes, has gained momentum in current research. Designing efficacious non-Pt metal agents that target DNA poses a complex challenge. In this study, we present the strategic design, synthesis, and thorough characterization of two innovative copper(II) complexes leveraging adenine as a ligand, a potential avenue to overcome these challenges. Our investigation demonstrates the superior cytotoxicity of these copper(II) complexes compared to the benchmark cisplatin, with complex C2 exhibiting the most promising anticancer activity, showcasing an impressive IC50 value of 4.51 µM in MGC-803 cells. Mechanistic insights underscore that complex C2 executes its cytotoxic effects by instigating DNA damage, orchestrating cell cycle arrest at the G2 phase, perturbing mitochondrial membrane potential, inducing ROS production, and ultimately triggering apoptotic pathways. These findings significantly emphasize the potential of designing novel adenine-based anticancer metal complexes targeting DNA, portraying a compelling trajectory for advancing anticancer drug development.
WITHDRAWN: Optimizing Agricultural Sustainability: A Comparative Analysis of Crop Plans through Goal Programming and R Programming
Authors: Polasi Sridevi , Shalini K 
Published: March 2024

Abstract
This study employs Goal Programming and R programming to compare and optimize various crop plans, aiming to enhance agricultural sustainability. The analysis considers multiple objectives, such as maximizing yield, minimizing resource usage, and promoting environmental conservation. The research utilizes mathematical modelling and computational techniques to evaluate and rank different crop plans, providing insights into their overall efficiency and ecological impact. In Uttara Kannada district, the study was conducted. In this study, both LINGO 19.0 and R programming were used to analyze the data, and both programming models yielded identical results.
Is Postoperative Routine Thoracic Imaging Necessary to Detect Thoracic Complications in Patients Undergoing Supracostal Mini Percutaneous Nephrolithotomy (M-pcnl) Surgery?
Authors: Esmeray Abdullah , Yazili Huseyin Burak , Gelmis Mucahit , Gunay Nazim Furkan , Dizdaroglu Caglar , Ozgor Faruk , Pazir Yasar , Caglar Ufuk 
Published: March 2024

Abstract
Purpose
This study aimed to assess the necessity of routine postoperative thoracic imaging for detecting pulmonary complications in patients undergoing supracostal mini percutaneous nephrolithotomy (m-pcnl) surgery.
Materials and Methods
Retrospective analysis was conducted on data from patients who underwent supracostal m-pcnl between 2017 and 2022 in a tertiary center. Excluding patients under 18, with kidney/skeletal anomalies, or active thoracic disease, 112 eligible patients were included. Patients were divided into two groups: those with routine postoperative chest X-rays (CXR) (Group 1, n = 40) and those without (Group 2, n = 72). Complications and operative data were compared between groups.
Results
Mean ages were 44.3 ± 11.4 (Group 1) and 42.6 ± 13.1 years (Group 2), with no significant difference (p = 0.102). Stone sizes were 30.8 ± 8.6 mm (Group 1) and 24.8 ± 8.4 mm (Group 2), also not significantly different (p = 0.313). High fever occurred in 10% of Group 1 and 4% of Group 2 (p = 0.246). Minimal effusion was found in 10% of Group 1, with no treatment due to lack of symptoms. However, subsequent CXR revealed hydropneumothorax in 2.5% of cases, necessitating thoracic tube insertion. In Group 2, 5% developed postoperative respiratory symptoms, with significant pneumothorax in 1.3%, requiring thoracic tube placement. Thoracic tube insertion rates did not significantly differ between groups (Group 1: 2.5% vs Group 2: 1.3%, p = 0.671).
Conclusion
Routine postoperative thoracic imaging did not show added benefit in detecting pulmonary complications post-supracostal m-pcnl.
Unveiling the Past: A Holistic Approach to Rescuing Historical Texts through Advanced Image Analysis and AI
Authors: Kadam Deepali , Jadhav Ayush , Govilkar Prathamesh , Bhosale Yash , Jadhav Shubham , Gamre Sahil 
Published: March 2024

Abstract
In recent years, more and more administrative and legal paperwork has been done using computers because it's efficient. But in the past, when technology wasn't as good, there were problems with printing documents. This meant that text could be hard to read, the ink might not be consistent, and documents could get damaged over time. This caused a lot of important information, especially in ancient scripts, to be lost because it couldn't be digitised. Our research is all about solving this problem. We want to find a way to automatically get accurate information from pictures of these old documents. We employ a multi-step approach to enhance text recovery. Firstly, we utilise adaptive Gaussian thresholding to improve image clarity by removing excess ink or stains. Next, we apply optical character recognition (OCR) using advanced systems like Easy OCR and Tesseract, followed by thorough database validation for result accuracy. But sometimes, even with the special software, we can't see all the words because they're too faded or missing. To overcome this, we employ Natural Language Processing (NLP) techniques with Happy Transformer, a tool based on Hugging Face's Transformer Library. This enables us to predict and reconstruct missing letters or words. After prediction and cross-referencing with the database, the identified words are seamlessly integrated into the text, ensuring data retrieval. Our research is important for preserving historical and legal documents that might otherwise be lost forever.
WITHDRAWN: Chemerin and Sfrp5 levels in Subclinical Hypothyroid
Authors: Şerefli Köksal , Bilgici Birşen , Akcan Songül , Tomak Leman , Atmaca Ayşegül Akın 
Published: March 2024

Abstract

Purpose
Thyroid dysfunction causes changes in adipokine and lipid profiles. Chemerin and Sfrp5 are adipokines with known effects on inflammation, but their relationship with thyroid hormones is not well clearly understood. This study aimed to investigate the effect of subclinical hypothyroidism on chemerine and Sfrp5 levels.
Methods
Serum chemerin, Sfrp5, LDL kolesterol, total kolesterol, trigliserid, HDL kolesterol, insulin, glucose, HOMA-IR, systolic-diastolic blood pressure, waist circumference, and BMI were measured in 46 subclinical hypothyroidism patients and in 49 healthy control subjects.
Results
While the patient group had higher serum chemerin levels and lower Sfrp5 levels compared to the control group, no statistically significant difference was found. When other parameters in the serum were examined, there was no significant difference between the groups. When both the patient and control groups were considered together, a significant positive correlation was found between chemerin and Sfrp5 levels and negative corelation was observed between Sfrp5 and LDL cholesterol, total cholesterol, triglycerides, BMI, diastolic blood pressure, systolic blood pressure, waist circumference values.
Conclusion
High levels of chemerin and low levels of Sfrp5 in subclinical hypothyroidism suggest that these two proteins may be associated with the disease. We found no statistically significant differences. This may be due to the fact that the participating patients had mild subclinical hypothyroidism and the inflammation was not at an advanced stage. Additionally, Sfrp5 is associated with metabolic syndrome parameters independent of subclinical hypothyroidism.
Unexpected plastic strain-induced phase transformation phenomena in silicon
Authors: Yesudhas Sorb , Levitas Valery I. , Lin Feng , Pandey K. K. , Smith Jesse S. 
Published: March 2024

Abstract

Pressure-induced phase transformations (PTs) in Si, the most important electronic material, have been broadly studied. However, strain-induced PTs in Si were never studied in situ. Here, we revealed in situ various important plastic strain-induced PT phenomena. A correlation between the particle size's direct and inverse Hall-Petch effect on yield strength and pressure for strain-induced PT is found. For 100 nm particles, strain-induced PT Si-I→Si-II initiates at 0.3 GPa versus 16.2 GPa under hydrostatic conditions; Si-I→Si-III PT starts at 0.6 GPa and does not occur under hydrostatic pressure. Pressure in small Si-II and Si-III regions is ~5-7 GPa higher than in Si-I. Retaining Si-II and single-phase Si-III at ambient pressure and obtaining reverse Si-II→Si-I PT demonstrates the possibilities of manipulating different synthetic paths. The obtained results corroborate the elaborated dislocation pileup-based mechanism and have numerous applications for developing economic defect-induced synthesis of nanostructured materials, surface treatment (polishing, turning, etc.), and friction.
Hazard-mapping and health risk analysis of iron and arsenic contamination in the groundwater of Sylhet district
Authors: Chowdhury Tahmida Naher , Hasan Md Mahmudul , Munna Gulam Md , Alam Md Jahir Bin , Nury Ahmad Hasan , Islam Shriful , Naher Tajmun 
Published: March 2024

Abstract

This study investigates groundwater contamination by arsenic and iron and its health implications within the Sylhet district in Bangladesh. Utilizing geographic information system (GIS) and inverse distance weighting (IDW) methods, hazard maps have been developed to evaluate contamination risk across various upazilas. The findings show significant arsenic and iron pollution, particularly in the northwestern part of the district. In about 50% of the area, especially in Jaintiapur, Zakiganj, Companiganj, and Kanaighat where arsenic levels surpass 0.05 mg/L which is the standard limit of Bangladesh. Iron levels peak at 13.83 mg/L, severely impacting 45% of the region, especially in Gowainghat, northeastern Jaintiapur, Zakigonj, and Golabganj. The study employs USEPA health risk assessment methods to calculate the hazard quotient (HQ) and hazard index (HI) for both elements via oral and dermal exposure. Results indicate that children face greater noncarcinogenic and carcinogenic risks than adults, with oral HI showing significant risk in Balagonj and Bishwanath. Dermal adsorption pathways exhibit comparatively lower risks. Cancer risk assessments demonstrate high carcinogenic risks from oral arsenic intake in all areas. This comprehensive analysis highlights the urgent need for effective groundwater management and policy interventions in the Sylhet district to mitigate these health risks and ensure safe drinking water.
Photoesterification of Waste Cooking Oil using ZnO Doped Empty
Fruit Bunches Ash Heterogeneous Catalyst
Authors: Mohd Shohaimi Norshahidatul Akmar , Adnan Norshahiera Khairul , Ibrahim Siti Fadhilah 
Published: March 2024

Introduction::
Waste from the palm oil industry, such as empty fruit
bunch ash (EFBA) and palm oil mill effluents (POME), is a type of biomass created
during the production of palm oil and produced in vast quantities. Due to the massive
amounts of empty fruit bunch ash produced because of the exponential rise in
worldwide palm oil production, major plantations are having trouble disposing of
them.

Aim::
The purpose of this research is to study the effectiveness of the ZnO-EFBA catalyst
under visible light irradiation for the photoesterification reaction and its physicochemical
properties of the photocatalyst that will be determined using TGA, SEMEDX,
XRD and BET.

Method::
The biodiesel will be produced by using two steps which are photoesterification
to reduce the FFA value in WCO and followed by transesterification to produce
FAMEs. The photoesterification reactions were conducted using WCO under visible
light irradiation. Various parameters were examined, including different reaction times
of 1 to 4 hours, different methanol to oil molar ratios of 12:1, 14:1, 16:1, and 18:1, and
different ZnO-EFBA catalyst loadings ranging from 0 wt.% to 8 wt.%. The obtained
results demonstrated that each WCO sample has a different optimum condition in the
photoesterification reaction. Moreover, it was observed that lower FFA values correlated
with higher biodiesel conversion rates in the transesterification reaction with
79.06%, 77.72% and 73.33% for samples 1, 2 and 3, respectively.

Result::
By using EFBA as a heterogeneous catalyst doped with ZnO in the manufacturing
of biodiesel, it helps to reduce the waste that the palm oil industry creates, limiting
the adverse effects on human health and environmental harm. Furthermore, biodiesel
is a renewable, clean-burning alternative to petroleum fuel, which is domestically
manufactured.

Conclusion::
The use of biodiesel as a vehicle fuel boosts energy security, enhances
the environment and air quality, and offers safety advantages.
Pengawasan Implementasi Peraturan Diseminasi Informasi Publik Terkait Covid-19 Kepada Masyarakat
Authors: Megawati Eka , Ifah Atur Kurniati  
Published: March 2024

Tujuan dari penelitian ini adalah untuk memahami bagaimana pengawasan implementasi peraturan diseminasi informasi publik terkait covid-19 kepada publik yang dilakukan oleh regulator dan lembaga pengawas, karena pendekatan yang digunakan untuk penelitian ini adalah metode kualitatif deskriptif, dengan teori terapan komunikasi krisis oleh Ludragen dan McMakin dengan asumsi teori mereka (1) Menentukan persepsi pemangku kepentingan dari berbagai faktor termasuk risiko, organisasi yang bertanggung jawab mengelola risiko, dan proses yang digunakan untuk mencapai keputusan (2) Menginformasikan, tidak membujuk (kecuali dalam konteks negosiasi yang disepakati) (3) Menyeimbangkan kebutuhan pemangku kepentingan yang bersaing (4) Membantu dalam mencapai resolusi yang semua pihak dapat hidup dengannya. Hasilnya menemukan bahwa pengawasan memang telah dilakukan dan terdapat sanksi diberikan bagi mereka pelanggar peraturan yang telah diregulasikan baik oleh Lembaga pengawasan seperti KPI maupun KOMINFO. Sejak awal, diseminasi informasi publik terkait covid-19 telah dimandatkan oleh Presiden RI bahwa agar informasi harus berasal dari satu pintu, namun dalam pelaksanaannya informasi dari satu pintu ini terbentur dengan kondisi demografis kasus covid-19 tiap daerah, dimana informasi terbagikan tak selalu sesuai kebutuhan maupun kendala dihadapi oleh pemerintah daerah, ditambah lagi masyarakat selaku publik banyak yang telah kehilangan kepercayaan terhadap Lembaga yang mendiseminasikan informasi publik serta kurangnya kemampuan dalam mencerna informasi tersebut.