bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quercetin sensitizes human hepatoma cells to TRAIL‐induced apoptosis via Sp1‐mediated DR5 up‐regulation and proteasome‐mediated c‐FLIPS down‐regulation
Authors: Kim Jin Yeop , Kim Eun Hee , Park Seok Soon , Lim Jun Hee , Kwon Taeg Kyu , Choi Kyeong Sook 
Published: November 2008

AbstractThis study demonstrates that combined treatment with subtoxic doses of quercetin (3′,3′,4′,5,7‐pentahydroxyflavone), a flavonoid found in many fruits and vegetables, plus tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) induces rapid apoptosis in TRAIL‐resistant hepatocellular carcinoma (HCC) cells. Effective induction of apoptosis by the combined treatment with quercetin and TRAIL was not blocked by overexpression of Bcl‐xL, which is known to confer resistance to various chemotherapeutic agents. These results suggest that this combined treatment may provide an attractive strategy for treating resistant HCCs. While the proteolytic processing of procaspase‐3 by TRAIL was partially blocked in various HCC cells treated with TRAIL alone, co‐treatment with quercetin efficiently recovered TRAIL‐induced caspase activation. We found that quercetin treatment of HCC cells significantly up‐regulated the mRNA and protein levels of DR5, a death receptor of TRAIL, in a transcription factor Sp1‐dependent manner. Furthermore, treatment with quercetin significantly decreased the protein levels of c‐FLIP, an inhibitor of caspase‐8, through proteasome‐mediated degradation. Finally, administration of small interfering RNA against DR5 or overexpression of c‐FLIPS, but not c‐FLIPL, significantly attenuated quercetin‐stimulated TRAIL‐induced apoptosis. Collectively, these findings show that quercetin recovers TRAIL sensitivity in various HCC cells via up‐regulation of DR5 and down‐regulation of c‐FLIPS. J. Cell. Biochem. 105: 1386–1398, 2008. © 2008 Wiley‐Liss, Inc.
DUB‐1, a fate determinant of dynein heavy chain in B‐lymphocytes, is regulated by the ubiquitin–proteasome pathway
Authors: Lee Min‐Young , Ajjappala Brijesh S. , Kim Myung‐Sun , Oh Yu‐Kyoung , Baek Kwang‐Hyun 
Published: November 2008

AbstractUbiquitinaiton and deubiquitination of post‐translational modification play counter roles in determining the fate of protein function in eukaryotic system for maintaining the cellular homeostasis. Even though novel family members of growth‐regulating deubiquitinating enzymes (DUB‐1 and DUB‐2) have been identified, their target proteins and functions are poorly understood. Dub genes encoding DUB‐1 and DUB‐2 are immediate‐early genes and are induced in response to cytokine stimuli rapidly and transiently. In order to explore the possible proteins regulated by DUB‐1, we performed the matrix assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) analysis followed by immunoprecipitation. We confirmed that DUB‐1 interacts with dynein heavy chain, which is known to regulate the movement of organelles and microtubule binding ability. In addition, structural and immunoprecipitation analyses revealed that DUB‐1 contains a putative PEST motif and is polyubiquitinated, indicating that DUB‐1 is also regulated by the ubiquitin–proteasome pathway. J. Cell. Biochem. 105: 1420–1429, 2008. © 2008 Wiley‐Liss, Inc.
Cell size increased in tissues from transgenic mice overexpressing a cell surface growth‐related and cancer‐specific hydroquinone oxidase, tNOX, with protein disulfide‐thiol interchange activity
Authors: Yagiz Kader , Snyder Paul W. , Morré D. James , Morré Dorothy M. 
Published: November 2008

AbstracttNOX (ENOX2), a cancer‐specific and growth‐related cell surface protein with protein disulfide‐thiol interchange and hydroquinone (NADH) oxidase activities was overexpressed in a transgenic mouse model. Female transgenic mice grew faster than wild type as did embryonic fibroblast cells prepared from the transgenic mice. The tissue expression of tNOX mRNA was greatest in heart, lung and liver. When these tissues were analyzed for cell size, the cells from the tissues of transgenic animals were, on average, 20% larger in surface area than cells from corresponding wild‐type tissues. Also analyzed were cells of intestine, spleen and kidney in which tNOX overexpression was observed but to a lesser extent. Cell size was increased as well with intestine and kidney but less so with spleen. At the end of the study, carcass weights of the transgenic animals were greater than those of wild type. This increase in carcass weight was reflected in an increase in femur weight and thickness in both male and female transgenic mice but not in femur length. Other carcass parameters such as skin weight and body fat or body fluids were unchanged or changes were insufficient to account for the increased carcass weight. The findings are consistent with the property of tNOX observed in studies with cultured cells as contributing to the enlargement phase of cell growth. J. Cell. Biochem. 105: 1437–1442, 2008. © 2008 Wiley‐Liss, Inc.
Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes
Authors: Miyoshi Hideaki , Perfield James W. , Obin Martin S. , Greenberg Andrew S. 
Published: November 2008

AbstractIn adipocytes, lipid droplet (LD) size reflects a balance of triglyceride synthesis (lipogenesis) and hydrolysis (lipolysis). Perilipin A (Peri A) is the most abundant phosphoprotein on the surface of adipocyte LDs and has a crucial role in lipid storage and lipolysis. Adipose triglyceride lipase (ATGL) and hormone‐sensitive lipase (HSL) are the major rate‐determining enzymes for lipolysis in adipocytes. Each of these proteins (Peri A, ATGL, and HSL) has been demonstrated to regulate lipid storage and release in the adipocyte. However, in the absence of protein kinase A (PKA) stimulation (basal state), the lipases (ATGL and HSL) are located mainly in the cytoplasm, and their contribution to basal rates of lipolysis and influence on LD size are poorly understood. In this study, we utilize an adenoviral system to knockdown or overexpress ATGL and HSL in an engineered model system of adipocytes in the presence or absence of Peri A. We are able to demonstrate in our experimental model system that in the basal state, LD size, triglyceride storage, and fatty acid release are mainly influenced by the expression of ATGL. These results demonstrate for the first time the relative contributions of ATGL, HSL, and Peri A on determination of LD size in the absence of PKA stimulation. J. Cell. Biochem. 105: 1430–1436, 2008. © 2008 Wiley‐Liss, Inc.
Plumbagin‐induced apoptosis of human breast cancer cells is mediated by inactivation of NF‐κB and Bcl‐2
Authors: Ahmad Aamir , Banerjee Sanjeev , Wang Zhiwei , Kong Dejuan , Sarkar Fazlul H. 
Published: November 2008

AbstractBreast cancer remains the major cause of cancer‐related deaths in women world‐wide. The heterogeneity of breast cancer has further complicated the progress of target‐based therapies. Triple negative breast cancers, lacking estrogen receptor, progesterone receptor and the Her‐2/neu (ErbB2), represent a highly aggressive breast cancer subtype, that are difficult to treat. Pleiotropic agents, such as those found in nature, can target receptor‐positive as well as receptor‐negative cancer cells, suggesting that such agents could have significant impact in breast cancer prevention and/or therapy. Plumbagin (5‐hydroxy‐2‐methyl‐1, 4‐naphthoquinone) is one such agent which has anti‐tumor activity against several cancers. However, its mechanism of action against breast cancer is not clearly understood. We hypothesized that plumbagin may act as an effective agent against breast cancer especially triple negative breast cancer. We tested our hypothesis using ER‐positive MCF‐7 and ER‐negative MDA‐MB‐231 (triple negative) breast cancer cells, and we found that plumbagin significantly inhibits the growth of breast cancer cells with no effect on normal breast epithelial cells. We also found that plumbagin induces apoptosis with concomitant inactivation of Bcl‐2 and the DNA binding activity of NF‐κB. Bcl‐2 over‐expression resulted in attenuation of plumbagin‐induced effects, suggesting that the inhibition of cell growth and induction of apoptosis by plumbagin is in part due to inactivation of NF‐κB/Bcl‐2 pathway. To our knowledge, this is the first report, showing mechanistic and cancer cell specific apoptosis‐inducing effects of plumbagin in breast cancer cells, suggesting the potential role of plumbagin in the prevention and/or treatment of breast cancer. J. Cell. Biochem. 105: 1461–1471, 2008. © 2008 Wiley‐Liss, Inc.
Human stem cells for modeling neurological disorders: Accelerating the drug discovery pipeline
Authors: Crook Jeremy Micah , Kobayashi Nao Rei 
Published: November 2008

AbstractThe availability of human stem cells heralds a new era for modeling normal and pathologic tissues and developing therapeutics. For example, the in vitro recapitulation of normal and aberrant neurogenesis holds significant promise as a tool for de novo modeling of neurodevelopmental and neurodegenerative diseases. Translational applications include deciphering brain development, function, pathologies, traditional medications, and drug discovery for novel pharmacotherapeutics. For the latter, human stem cell‐based assays represent a physiologically relevant and high‐throughput means to assess toxicity and other undesirable effects early in the drug development pipeline, avoiding late‐stage attrition whilst expediting proof‐of‐concept of genuine drug candidates. Here we consider the potential of human embryonic, adult, and induced pluripotent stem cells for studying neurological disorders and preclinical drug development. J. Cell. Biochem. 105: 1361–1366, 2008. © 2008 Wiley‐Liss, Inc.
Sneaking up and stumbling back: Textiles sector performance under crisis conditions in Zimbabwe
Authors: Chiripanhura Blessing M. 
Published: November 2008

AbstractThe paper examines the structure and performance of the textiles and clothing sectors in Zimbabwe. Based on firm level data, it examines the challenges and factors behind the resilience of the two sectors. It shows that increased flexibility, reduced capacity utilisation, modernisation of production systems and production incentives were among the most important factors exploited by firms to remain in business. It shows the main hindrance as macroeconomic instability that caused raw materials and skills shortages. It concludes that the sectors still have comparative advantage that can be exploited in a stable economy. The challenge is that the longer the current crisis goes unresolved, the more likely it is that the sectors will lose all the potential comparative advantage, implying that firms that have survived the crisis since 1997 may eventually be forced to shut down. Copyright © 2008 John Wiley & Sons, Ltd.
Microwave‐accelerated synthesis of psychoactive deuterated N,N‐dialkylated‐[α,α,β,β‐d4]‐tryptamines
Authors: Brandt Simon D. , Tirunarayanapuram Sri Subha , Freeman Sally , Dempster Nicola , Barker Steven A. , Daley Paul F. , Cozzi Nicholas V. , Martins Cláudia P. B. 
Published: November 2008

AbstractA large number of N,N‐dialkylated tryptamines are known to induce psychoactive effects in humans. This has resulted in their increased attention within clinical and forensic communities. Deuterated tryptamines are ideal for use as internal standards during MS bioanalysis or of use in biochemical NMR studies. The present study reports on a microwave‐enhanced synthesis of 22 N,N‐dialkylated‐[α,α,β,β‐d4]‐tryptamines via the reduction with lithium aluminium deuteride of glyoxalylamide precursors obtained by the procedure of Speeter and Anthony. Syntheses were carried out using a single‐mode system under elevated pressure conditions where anhydrous tetrahydrofuran was used as the solvent at 150°C. Good yields were obtained within 5 min. Copyright © 2008 John Wiley & Sons, Ltd.
High‐quality relationships, psychological safety, and learning from failures in work organizations
Authors: Carmeli Abraham , Gittell Jody Hoffer 
Published: November 2008

AbstractHow can organizations support employees to engage in learning from failures? In this paper, we draw on the concept of high‐quality relationships to explore the relational underpinnings of learning from failures in organizations. We focus on relational coordination as a specific manifestation of high‐quality relationships and examine how the relational dimensions of relational coordination—shared goals, shared knowledge, and mutual respect—foster psychological safety and thus enable organizational members to engage in learning from failures. The results of two separate studies support our mediation model where psychological safety mediates the link between high‐quality relationships and learning from failures in organizations. Copyright © 2008 John Wiley & Sons, Ltd.
An examination of the factorial, construct, and predictive validity and utility of the regulatory focus at work scale
Authors: Wallace J. Craig , Johnson Paul D. , Frazier M. Lance 
Published: November 2008

AbstractAlthough interest in regulatory focus research continues to grow, there has been very little work to date on the assessment and usefulness of regulatory focus in the workplace. The present research effort reports the results of a series of studies designed to assess the validity and utility of the Regulatory Focus at Work Scale (RWS). The results indicate that the RWS is reliable, valid, and showed incremental validity beyond general and trait‐like measures of regulatory focus in predicting relevant work outcomes of task performance, citizenship performance, safety performance, and productivity performance. The overall results of these studies support our claims that the RWS shows utility in predicting workplace outcomes and should prove useful for future research on regulatory focus in the workplace. Copyright © 2008 John Wiley & Sons, Ltd.
Reference database of Raman spectra of pharmaceutical excipients
Authors: de Veij Marleen , Vandenabeele Peter , De Beer Thomas , Remon Jean Paul , Moens Luc 
Published: November 2008

AbstractRaman spectroscopy has evolved into an important fast, direct and nondestructive technique in pharmaceutical analysis. Usually, the focus in this field is mainly on the active ingredients and not on the excipients present in the drugs. A collection of Raman spectra of widely used pharmaceutical excipients is presented in this article, which can serve as a reference for the interpretation of Raman spectra during drug analysis (including classical qualitative and quantitative pharmaceutical analysis, counterfeit tracing and process analytical technology (PAT) applications). The 43 analyzed excipients can be classified into seven categories: mono‐ and disaccharides (dextrose, lactitol, maltitol, lactose and sucrose), polysaccharides (microcrystalline cellulose, methylcellulose (MC), carboxymethylcellulose (CMC), hydroxypropyl cellulose (HPC), hydroxypropyl methylcellulose (HPMC), wheat starch, maltodextrin, primojel, tragacanth and pectin), polyalcohols (propylene glycol, erythritol, xylitol, mannitol and sorbitol), carboxylic acids and salts (alginic acid, glycine, magnesium stearate, sodium acetate and sodium benzoate), esters (arachis oil, lubritab, dibutyl sebacate, triacetin, Eudragit E100 and Eudragit RL100), inorganic compounds (calcium phosphate, talc, anatase and rutile (TiO2), calcium carbonate, magnesium carbonate, sodium bicarbonate and calcium sulfate) and some unclassified products [gelatin, macrogol 4000 (polyethylene glycol (PEG), polyvinyl pyrrolidone and sodium lauryl sulfate]. Copyright © 2008 John Wiley & Sons, Ltd.
Effect of chemical structure on film‐forming properties of seed oils
Authors: Biresaw G. , Bantchev G. 
Published: November 2008

AbstractThe film thickness of seven seed oils and two petroleum‐based oils of varying chemical structure was investigated by the method of optical interferometry. The measured film thickness (hmeas) was then compared to that calculated using the Hamrock–Dowson (H‐D) equation. The result showed that the H‐D equation adequately and without exception predicted hmeas in the high entrainment speed region but not in the low entrainment speed region. In order to understand these deviations further, the chemical structural variabilities of the oils were quantified using empirical parameters, and its effect on film thickness in the low entrainment speed region was examined. The result of this examination indicated that, in the low entrainment speed region, the film thickness of seed oils (i) rarely correlates with viscosity as proposed by the H‐D equation, (ii) increases with decreasing polarity of the oil, and (iii) increases with decreasing degree of unsaturation of the oils. Published in 2008 by John Wiley and Sons, Ltd.
Clinical analysis and surgical results of cardiac myxoma in pediatric patients
Authors: Wu Kai Hong , Mo Xu Ming , Liu Ying Long 
Published: November 2008

AbstractBackgroundCardiac myxoma, the most common primary tumor in the adult, is less often encountered in infants and children. We reviewed our series of children with cardiac myxoma over a 22‐year period and present the long‐term results of surgical resection of the tumor.Patients and MethodsFrom January 1985 to December 2006, 15 children between the ages of 5 months and 14 years (mean age, 6.5 years) with cardiac myxoma were reviewed. Follow‐up information from every patient was obtained from outpatient records and direct patient contact.ResultsCardiac signs appeared in 86.7% of the patients. Most myxomas originated from the left atrium. There was no operative death. No patients were lost to follow‐up, and the mean follow‐up was 9.6 years. There were no late deaths during the follow‐up. To date, 13 of 15 operative survivors were in New York Heart Association (NYHA) functional class I without medication and the other 2 were in class II. All patients demonstrated sinus rhythm and no child required reexcision of tumor.ConclusionsOur clinical experience showed surgical resection was a safe and effective treatment for cardiac myxoma in pediatric patients and the risk of recurrence was low during the long term follow‐up. J. Surg. Oncol. 2009;99:48–50. © 2008 Wiley‐Liss, Inc.
Does chemotherapy increase morbidity and mortality after pneumonectomy?
Authors: Alan Stolz , Jan Simonek , Tomas Harustiak , Robert Lischke , Jan Schutzner , Pavel Pafko 
Published: November 2008

AbstractBackgroundThe impact of chemotherapy on postoperative complications after pneumonectomy remains unclear, and the procedure is often considered as potentially dangerous.MethodsThis retrospective review of prospectively collected data included 269 patients operated from January 1, 1998 through December 31, 2007. Group 1 included patients those who received preoperative chemotherapy (PCT) and pneumonectomy (54 patients, 20%), and group 2 included patients who underwent pneumonectomy alone (215 patients, 80%).ResultsOverall 30‐ and 90‐day mortality rates were 5.9% and 8.9%, respectively. Postoperative mortality at 30 days was 5.6% in group 1 and 6% in group 2 (P = 0.16), and 11.1% for group 1 and 8.3% in group 2 at 90 days (P = 0.8). Incidence of postoperative respiratory failure was 3.7% in group 1 and 3.2% in group 2 (P = 0.62); incidence of empyema was 5.5% in group 1 and 2.8% in group 2 (P = 0.1). Incidence of bronchopleural fistula (BPF) was 3.7% in group 1 and 2.8% in group 2 (P = 0.55); risk of BPF was higher following right pneumonectomy (6.1%) versus left pneumonectomy (0%, P = 0.003).ConclusionsPCT does not significantly increase postoperative morbidity and early mortality after pneumonectomy in our experience. J. Surg. Oncol. 2009;99:38–41. © 2008 Wiley‐Liss, Inc.
Rapid SPE–HPLC determination of the 16 European priority polycyclic aromatic hydrocarbons in olive oils
Authors: Purcaro Giorgia , Moret Sabrina , Conte Lanfranco S. 
Published: November 2008

AbstractPolycyclic aromatic hydrocarbons (PAHs) are a large class of organic compounds. It has been established that the main source of exposure to these compounds for human beings is through food, particularly fats and oils, due to the lipophilic nature of these polycyclic compounds. The aim of this work was to optimise and validate a method involving SPE and HPLC for rapid determination of the 16 European Union (EU) priority PAHs (required by the Recommendation 2005/108/EC) in vegetable oils. Two spectrofluorometric detectors and a UV‐Visible detector in series were used to identify and quantify the target compounds. Linearity, recoveries, LOD, and LOQ were found to be in agreement with the performance criteria for benzo[a]pyrene (BaP) analysis as required by the Commission Directive 2005/10/EC, and satisfactory for all the compounds of interest, except for cyclopenta[c,d]pyrene, which presented a very low signal in the UV. Optimised chromatographic conditions for the separation of 25 PAHs, comprising both EPA and EU priority PAHs plus benzo[e]pyrene and benzo[b]chrysene, have been also proposed.
Evaluating urbanization, fragmentation and land use/land cover change pattern in Istanbul city, Turkey from 1971 TO 2002
Authors: Çakir G. , Ün C. , Baskent E. Z. , Köse S. , Sivrikaya F. , Keleş S. 
Published: November 2008

AbstractSpatiotemporal analysis of landscape dynamics is crucial in formulating an appropriate set of actions in landscape management. This paper presents a large scale analysis of the spatiotemporal structure of Istanbul, a highly urbanized city in Turkey, from 1971 to 2002 using forest cover type maps analysed with geographical information systems (GIS) and a spatial statistics programme. The quantitative evidence indicated that increasing population and expanding urbanization caused drastic changes to the temporal and spatial dynamics of land use/land cover pattern in Istanbul. There was a net increase of 5387·3 ha in total forested areas (1·0 per cent) due to mainly reforestation activities even though the population increased three times over a 31‐year period. Increase in number of patches and decrease in mean patch size together demonstrated that the landscape developed into a more fragmented structure that would negatively affect biodiversity and the resilience of the ecosystems. In conclusion, plain increase in forest areas may not always be a favourable situation. The quality, composition and the configuration of forest landscape should also be analysed to present the dynamics of ecosystem in terms of ecological and economical sustainability over a longer time and larger area. Copyright © 2008 John Wiley & Sons, Ltd.
Complete 1H and 119Sn NMR spectral assignment for an asymmetric di[dihydroxotin(IV)] bis‐porphyrin supramolecular host and its corresponding tetraacetato complex
Authors: Brotherhood Peter R. , Luck Ian J. , Crossley Maxwell J. 
Published: November 2008

AbstractThe full 1H and 119Sn NMR spectral assignments for a di[dihydroxotin(IV)] bis‐porphyrin supramolecular host I and for the di[diacetatotin(IV)] complex II are presented. Despite the lack of varied chemical functionality in these molecules, all of their 64 proton environments are non‐equivalent. This is due to the asymmetry afforded by the Tröger's base (methanodiazocine) bridge between the porphyrin and quinoxalinoporphyrin macrocycles. The methanodiazocine bridge imparts chirality and concavity on the host framework and the quinoxalino link to one porphyrin macrocycle results in a negation of C2 symmetry. The anisotropy of the aromatic porphyrin and quinoxalinoporphyrin macrocycles results in good dispersion for all 60 signals of the host framework and for the four ligands bound in the axial positions of the tin(IV) centres. The full assignment of the 1H NMR spectra for these systems was achieved using dqf‐COSY, NOESY, ROESY, 1H‐119Sn HMQC, 1H‐13C HSQC and 1H‐13C HMBC spectroscopy at temperatures that optimised dispersion. The 1H‐119Sn HMQC was particularly useful in this assignment. The 119Sn chemical shift is sensitive to the functionality of the porphyrin and to the nature of the axial ligation, and the 119Sn centre couples to both the ligand protons and the β‐pyrrolic protons. This allows unequivocal identification of the spin systems associated with each metal centre. Copyright © 2008 John Wiley & Sons, Ltd.