bluuebunny/crossref_metadata_2025_split huggingface dataset:
Joint effects of indoor air pollution and maternal psychosocial factors during pregnancy on trajectories of early childhood psychopathology
Authors: Christensen Grace M , Marcus Michele , Vanker Aneesa , Eick Stephanie M , Malcolm-Smith Susan , Suglia Shakira F , Chang Howard H , Zar Heather J , Stein Dan J , Hüls Anke 
Published: April 2024

Abstract
Prenatal indoor air pollution and maternal psychosocial factors have been associated with adverse psychopathology. We used environmental-exposure mixture methodology to investigate joint effects of both exposure classes on child behavior trajectories. For 360 children from the South African Drakenstein Child Health Study, we created trajectories of Child Behavior Checklist scores (at 24, 42, and 60 months) using latent-class linear mixed effects models. Indoor air pollutants and psychosocial factors were measured during pregnancy (second trimester). After adjusting for confounding, single-exposure effects (per natural log-1 unit increase) were assessed using polytomous logistic regression models, joint effects using self-organizing maps, and principal component analysis. Three trajectories were chosen for both internalizing and externalizing problems, with “high” (externalizing) or “increasing” (internalizing) being the most adverse trajectories. High externalizing trajectory was associated with increased exposure to particulate matter of ≤ 10 microns in diameter (PM10) (odds ratio [OR] = 1.25; 95% CI, 1.01-1.55) and self-organizing maps exposure profile most associated with smoking (OR = 2.67; 95% CI, 1.14-6.27). Medium internalizing trajectory was associated with increased emotional intimate partner violence (OR = 2.66; 95% CI, 1.17-5.57), increasing trajectory with increased benzene (OR = 1.24; 95% CI, 1.02-1.51) and toluene (1.21; 95% CI, 1.02-1.44) and the principal component most correlated with benzene and toluene (OR = 1.25; 95% CI, 1.02-1.54). Prenatal exposure to environmental pollutants and psychosocial factors was associated with internalizing and externalizing child behavior trajectories. Understanding joint effects of adverse exposure mixtures will facilitate targeted interventions to prevent childhood psychopathology.
This article is part of a Special Collection on Mental Health.
Alcohol to-go sales policies at on-premise drinking establishments near large public US universities during the COVID-19 pandemic
Authors: Rosen Erika M , Walker Andrew , Trangenstein Pamela J , LoParco Cassidy R , Livingston Melvin D , Barry Adam E , Rossheim Matthew E 
Published: April 2024

Abstract

Aims
Sweeping policy changes during the COVID-19 pandemic increased alcohol availability through permitted to-go sales, potentially posing unique risks to college students. While to-go sales may make binge drinking more convenient, little remains known about these practices. Therefore, this study aimed to assess whether drinking establishments’ to-go sales practices are associated with their other operational practices and state policy.

Method
This cross-sectional analysis included 221 randomly selected bars, nightclubs, and restaurants within two miles of a large public university. Telephone interviews assessed establishment practices, and the Alcohol Policy Information System provided state alcohol to-go laws. Regression models tested whether establishment to-go sales practices were associated with their business practices (logistic regression) and state policy (generalized estimating equations).

Results
Nearly one-half (44.8%) of drinking establishments sold alcohol to-go. Establishments with higher vodka prices had nearly 30% higher odds of selling spirits to-go (aOR = 1.29) and establishments offering happy hours specials had more than twice the odds of selling beer (aOR = 2.22), wine (aOR = 2.53), and spirits to-go (aOR = 2.60). Additionally, establishments that implemented physical distance requirements had higher odds of selling wine to-go (aOR = 3.00). State to-go laws were associated with higher odds of selling wine (aOR = 3.99) and spirits to-go (aOR = 5.43) in the full sample and beer to-go (aOR = 4.92) in urban counties.

Conclusions
Establishments that sell alcohol to-go tend to engage in other practices designed to drive sales. Evaluations of alcohol to-go sales laws on risky consumption among priority populations, including college students, are urgently needed to inform decisions about how to appropriately regulate sales.
Efficient cytometry analysis with FlowSOM in Python boosts interoperability with other single-cell tools
Authors: Couckuyt Artuur , Rombaut Benjamin , Saeys Yvan , Van Gassen Sofie 
Published: April 2024

Abstract

Motivation
We describe a new Python implementation of FlowSOM, a clustering method for cytometry data.

Results
This implementation is faster than the original version in R, better adapted to work with single-cell omics data including integration with current single-cell data structures and includes all the original visualizations, such as the star and pie plot.

Availability and implementation
The FlowSOM Python implementation is freely available on GitHub: https://github.com/saeyslab/FlowSOM_Python.
Integrative annotation scores of variants for impact on RNA binding protein activities
Authors: Duan Jingqi , Gasch Audrey P , Keleş Sündüz 
Published: April 2024

Abstract

Motivation
The ENCODE project generated a large collection of eCLIP-seq RNA binding protein (RBP) profiling data with accompanying RNA-seq transcriptomes of shRNA knockdown of RBPs. These data could have utility in understanding the functional impact of genetic variants, however their potential has not been fully exploited. We implement INCA (Integrative annotation scores of variants for impact on RBP activities) as a multi-step genetic variant scoring approach that leverages the ENCODE RBP data together with ClinVar and integrates multiple computational approaches to aggregate evidence.

Results
INCA evaluates variant impacts on RBP activities by leveraging genotypic differences in cell lines used for eCLIP-seq. We show that INCA provides critical specificity, beyond generic scoring for RBP binding disruption, for candidate variants and their linkage-disequilibrium partners. As a result, it can, on average, augment scoring of 46.2% of the candidate variants beyond generic scoring for RBP binding disruption and aid in variant prioritization for follow-up analysis.

Availability and implementation
INCA is implemented in R and is available at https://github.com/keleslab/INCA.
Association between repeat imaging and readmission in patients with acute ischaemic stroke: a 16-year nationwide population-based study
Authors: Liang Han-Hsuan , Liu Hung-Yi , Kosik Russell Oliver , Chan Wing P , Chien Li-Nien 
Published: April 2024

Abstract

Objectives
This study aims to evaluate such usage patterns and identify factors that may contribute to the need for repeat imaging in acute ischaemic stroke patients and determine the association between repeat imaging and readmission in Taiwan.

Methods
We searched and analysed data from the Taiwan National Health Insurance Research Database for patients admitted for acute ischaemic stroke between 2002 and 2017. Cases where repeat brain imaging during the initial hospital admission occurred and where patients were readmitted within 30 days following discharge were documented.

Results
Of a total of 195 016 patients with new onset ischaemic stroke, 51 798 (26.6%) underwent repeat imaging during their initial admission. Factors associated with repeat brain imaging included younger age, longer hospital stay, use of recombinant tissue plasminogen activator (rt-PA) therapy (odds ratio = 2.10 [95% CI, 1.98-2.22]), more recent year of diagnosis, higher National Institutes of Health Stroke Scale (NIHSS) score, and admission to a hospital offering a higher level of care. Repeat imaging was also associated with an increased risk of ischaemic stroke and all types of stroke readmission.

Conclusions
Repeat brain imaging of patients with stroke has increased in recent years, and it is associated with certain factors including age, length of stay, use of rt-PA, hospital level of care, and NIHSS score. It is also associated with increased readmission.

Advances in knowledge
Knowledge of the associations of repeat imaging may help clinicians use repeat imaging more carefully and efficaciously.
Different learning aberrations relate to delusion-like beliefs with different contents
Authors: Rossi-Goldthorpe Rosa , Silverstein Steven M , Gold James M , Schiffman Jason , Waltz James A , Williams Trevor F , Powers Albert R , Woods Scott W , Zinbarg Richard E , Mittal Vijay A , Ellman Lauren M , Strauss Gregory P , Walker Elaine F , Levin Jason A , Castiello Santiago , Kenney Joshua , Corlett Philip R 
Published: April 2024

Abstract
The prediction error account of delusions has had success. However, its explanation of delusions with different contents has been lacking. Persecutory delusions and paranoia are the common unfounded beliefs that others have harmful intentions towards us. Other delusions include believing that one's thoughts or actions are under external control or that events in the world have specific personal meaning.
We compare learning in two different cognitive tasks, probabilistic reversal learning and Kamin blocking, that have relationships to paranoid and non-paranoid delusion-like beliefs, respectively.
We find that clinical high-risk status alone does not result in different behavioural results in the probabilistic reversal learning task but that an individual's level of paranoia is associated with excessive switching behaviour. During the Kamin blocking task, paranoid individuals learned inappropriately about the blocked cue. However, they also had decreased learning about the control cue, suggesting more general learning impairments. Non-paranoid delusion-like belief conviction (but not paranoia) was associated with aberrant learning about the blocked cue but intact learning about the control cue, suggesting specific impairments in learning related to cue combination. We fit task-specific computational models separately to behavioural data to explore how latent parameters vary within individuals between tasks and how they can explain symptom-specific effects. We find that paranoia is associated with low learning rates in the probabilistic reversal learning task and the blocking task. Non-paranoid delusion-like belief conviction is instead related to parameters controlling the degree and direction of similarity between cue updating during simultaneous cue presentation.
These results suggest that paranoia and other delusion-like beliefs involve dissociable deficits in learning and belief updating, which, given the transdiagnostic status of paranoia, might have differential utility in predicting psychosis.
Computational screening of PCP-type pincer ligands for Mo-catalyzed nitrogen fixation
Authors: Egi Akihito , Tanaka Hiromasa , Nakamura Taiji , Arashiba Kazuya , Nishibayashi Yoshiaki , Yoshizawa Kazunari 
Published: April 2024

Abstract
Computational screening of N-heterocyclic carbene-based PCP-type pincer ligands (PCP = 1,3-bis(di-tert-buthylphosphinomethyl)benzimidazole-2-ylidene) has been performed for the design of molybdenum-based molecular catalysts for nitrogen fixation. Previously, we theoretically and experimentally demonstrated that the introduction of electron-donating/withdrawing substituents to the original PCP ligand is a promising way to control the catalytic activity. Here, we investigate electronic and energetic properties of nitrogenous Mo intermediates bearing 40 substituted PCP ligands [MoI(NHx)(R-PCP)] (x = 1 to 3) that are involved in the rate-determining step in our proposed catalytic mechanism. Electron-withdrawing substituents enhance the π-accepting ability of R-PCP and effectively stabilize the lowest unoccupied molecular orbital (LUMO) of the corresponding Mo–nitride (Mo≡N) complexes, which is expected to be advantageous for the transformation of the nitride N atom via proton-coupled electron transfer. The introduction of strong electron-withdrawing substituents to the PCP ligand also increases the N–H bond energy of [MoI(NHx)(R-PCP)] evaluated using the bond dissociation free energy and the bond dissociation enthalpy. As a result of the computational screening, we newly propose an alternative strategy for designing PCP ligands with high π-accepting ability: the extension of the π-conjugated system of the PCP ligand by introducing fused benzene rings.
Excimer laser coronary angioplasty without stenting in a 37-year-old man with acute coronary syndrome involving left main trunk: a case report
Authors: Shigeno Ryo , Hirohata Atsushi 
Published: April 2024

AbstractBackgroundExcimer laser coronary angioplasty (ELCA) is utilized to reduce thrombus in acute coronary syndrome (ACS). However, the feasibility and safety of ELCA for patients with ACS involving the left main trunk (LMT) and bifurcation, as well as the safety of a stentless strategy with ELCA, are not well-documented.Case summaryA 37-year-old man without any past medical history presented with chest pain. Electrocardiogram showed ST-segment elevation in leads I, aVL, and V2–V6. Emergent coronary angiography (CAG) showed a 99% stenosis from LMT to proximal left anterior descending artery (LAD). Intra-aortic balloon pumping (IABP) was initiated. Intravascular ultrasound revealed massive thrombus at the culprit lesion. Thrombus aspiration was not enough to reduce the thrombus, thus, we conducted thrombus vaporization with a 0.9 mm ELCA catheter. Coronary angiography after the procedure showed reduced thrombus with thrombolysis in myocardial infarction grade 3 flow. Considering his age and the complexity of stenting the LMT, we completed the procedure without stenting. After the intervention, we initiated triple antithrombotic therapy. On Day 3, we removed the IABP. On Day 11, CAG showed no significant stenosis. Optical coherence tomography revealed ulceration, indicating the presence of plaque disruption at the proximal LAD as the likely cause of thrombosis. With improvement in CAG findings, we stopped heparin and continued dual antiplatelet therapy. He was discharged on Day 20.DiscussionExcimer laser coronary angioplasty without stenting can be an option for the patients with ACS involving LMT, especially for younger patients who are suitable to avoid stenting on bifurcation lesions for lifelong management.
The P2X7 Receptor is a Master Regulator of Microparticle and Mitochondria Exchange in Mouse Microglia
Authors: Falzoni Simonetta , Vultaggio-Poma Valentina , Chiozzi Paola , Tarantini Mario , Adinolfi Elena , Boldrini Paola , Giuliani Anna Lisa , Morciano Giampaolo , Tang Yong , Gorecki Dariusz C , Di Virgilio Francesco 
Published: April 2024

Abstract
Microparticles (MPs) are secreted by all cells, where they play a key role in intercellular communication, differentiation, inflammation, and cell energy transfer. P2X7 receptor (P2X7R) activation by extracellular ATP (eATP) causes a large MP release and affects their contents in a cell-specific fashion. We investigated MP release and functional impact in microglial cells from P2X7R-WT or P2X7R-KO mice, as well as mouse microglial cell lines characterized for high (N13-P2X7RHigh) or low (N13-P2X7RLow) P2X7R expression. P2X7R stimulation promoted release of a mixed MP population enriched with naked mitochondria. Released mitochondria were taken up and incorporated into the mitochondrial network of the recipient cells in a P2X7R-dependent fashion. NLRP3 and the P2X7R itself were also delivered to the recipient cells. Microparticle transfer increased the energy level of the recipient cells and conferred a pro-inflammatory phenotype. These data show that the P2X7R is a master regulator of intercellular organelle and MP trafficking in immune cells.
Validation of large animal models in mechanical valve research: a histologic comparison
Authors: Van Hecke Manon , Langenaeken Tom , Rega Filip , Roskams Tania , Meuris Bart 
Published: April 2024

Abstract

OBJECTIVES
Mechanical valves still require life-long anticoagulation. Preclinical animal testing is a crucial step in the assessment of valves; however, the chosen animal model should be carefully considered, and a well-controlled animal model of mechanical valve thrombosis has not been established yet. In this study, a histopathologic comparison was performed to evaluate the representativity of pigs and sheep as large animal models in bileaflet mechanical valve thrombosis research.

METHODS
10 pigs and 8 sheep were implanted with a bileaflet mechanical valve in pulmonary position. During follow-up, no anticoagulative therapy was administered. Pigs were sacrificed between 14 and 38 days for explantation and assessment of the valve. Sheep were sacrificed between 71 and 155 days. Thrombus samples were processed and (immuno)histochemical stainings were applied. A pathologist evaluated the samples morphologically and semiquantitatively and compared these samples to available slides from 3 human patients who underwent redo surgery for acute bileaflet mechanical valve thrombosis, caused by insufficient anticoagulation.

RESULTS
All pigs showed macroscopically evident thrombi on the mechanical valve surface at sacrifice. In contrast, none of the sheep showed any sign of thrombus formation. Histology showed a high fibrin content in thrombi of both human and porcine cases (3/3 vs 8/10). Porcine thrombi showed more cellular organization (0/3 vs 6/10), more calcification (0/3 vs 9/10) and more endothelialization (0/3 vs 6/10). Inflammatory cells were present in all samples and were considered physiological.

CONCLUSIONS
Contrary to sheep, pigs develop thrombi on their mechanical valves in the short-term if no anticoagulation is administered. Histologic comparison of human and porcine thrombi shows comparable findings. The pig model might serve interestingly for further research on valve thrombosis, if it shows not to be an overly aggressive model.
Surgical techniques using an attached patch for large left ventricular pseudoaneurysm after inferior acute myocardial infarction
Authors: Yamada Ryotaro , Okamura Homare , Wada Yohnosuke , Yamaguchi Atsushi 
Published: April 2024

Abstract
We report a case of large left ventricular pseudoaneurysm after inferior acute myocardial infarction. Patch repair is commonly performed; however, only a few studies have described specific surgical techniques for left ventricular pseudoaneurysm repair of the inferior left ventricular wall. As an optimal repair technique for left ventricular pseudoaneurysm of the inferior left ventricular wall is lacking, we believe our technique is safe and effective in repairing this pathology.
Long-term exposure to ambient air pollution and risk of microvascular complications among patients with type 2 diabetes: a prospective study
Authors: Wang Bin , Sun Ying , Zhang Kun , Wang Yuying , Tan Xiao , Wang Ningjian , Lu Yingli 
Published: April 2024

Abstract

Background
Patients with type 2 diabetes (T2D) may disproportionately suffer the adverse cardiovascular effects of air pollution, but relevant evidence on microvascular outcome is lacking. We aimed to examine the association between air pollution exposure and the risk of microvascular complications among patients with T2D.

Methods
This prospective study included 17 995 participants with T2D who were free of macro- and micro-vascular complications at baseline from the UK Biobank. Annual average concentrations of particulate matter (PM) with diameters &lt;2.5 μm (PM2.5), &lt;10 μm (PM10), nitrogen dioxide (NO2) and nitrogen oxides (NOx) were assessed using land use regression models. Cox proportional hazards regression was used to estimate the associations of air pollution exposure with incident diabetic microvascular complications. The joint effects of the air pollutant mixture were examined using quantile-based g-computation in a survival setting.

Results
In single-pollutant models, the adjusted hazard ratios (95% confidence intervals) for composite diabetic microvascular complications per interquartile range increase in PM2.5, PM10, NO2 and NOx were 1.09 (1.04–1.14), 1.06 (1.01–1.11), 1.07 (1.02–1.12) and 1.04 (1.00–1.08), respectively. Similar significant results were found for diabetic nephropathy and diabetic neuropathy, but not for diabetic retinopathy. The associations of certain air pollutants with composite microvascular complications and diabetic nephropathy were present even at concentrations below the World Health Organization limit values. Multi-pollutant analyses demonstrated that PM2.5 contributed most to the elevated risk associated with the air pollutant mixture. In addition, we found no interactions between air pollution and metabolic risk factor control on the risk of diabetic microvascular complications.

Conclusions
Long-term individual and joint exposure to PM2.5, PM10, NO2 and NOx, even at low levels, was associated with an increased risk of diabetic microvascular complications, with PM2.5 potentially being the main contributor.
High Art, High Artists
Authors: Fokt Simon 
Published: April 2024

Abstract
Artists rarely shy away from a drink and other psychoactive substances, yet it seems that there has never been much discussion on what aesthetic or artistic relevance this has to their works and their reception. I outline the scale of the phenomenon focusing on some prominent examples and distinguish a subset of what I call ‘high artworks’. In such artworks, I argue, drug experiences are encoded: their drug-related contextual and intrinsic properties or content are aesthetically or artistically relevant and should be mentioned in any in-depth analysis. I then further argue that it is impossible or at least very difficult to fully appreciate or produce optimal evaluations of a high artwork, unless one has oneself had drug-induced experiences of the kind encoded in the work. This is because such experiences afford one the relevant phenomenal knowledge that is otherwise inaccessible, yet required to gain an adequate level of competence allowing one to fully grasp the work.
Posaconazole in paediatric malignancy and haematopoietic stem cell transplant: dosing to achieve therapeutic concentration
Authors: Weerdenburg Heather , Walker Hannah , Curtis Nigel , Duffull Stephen , Haeusler Gabrielle , Cole Theresa , Gwee Amanda 
Published: April 2024

Abstract

Objectives
Posaconazole is increasingly used for the treatment and prophylaxis of invasive fungal infections in immunocompromised children. We aimed to review evidence for paediatric posaconazole dosing regimens focusing on attainment of target concentrations and frequency of adverse effects.

Methods
In May 2023, the Cochrane, Embase, MEDLINE and PubMed databases were searched for articles reporting posaconazole dosing in children with malignancy or post-haematopoietic stem cell transplantation. Studies reporting the attainment of target serum concentrations were included.

Results
Overall, 24 studies were included. Eighteen studies of the oral suspension consistently reported poor attainment of target concentrations for prophylaxis (≥0.7 µg/mL, 12%–78%) despite high daily doses of 14–23 mg/kg/day (max. 1200 mg/day). Target attainment was significantly affected by gastric pH and food intake. Six studies of the delayed-release tablet (DRT) reported 58%–94% achieved concentrations ≥0.7 µg/mL, with the majority using lower doses of 4–12 mg/kg/day (max. 300 mg/day). Similarly, one study of powder for oral suspension found 67%–100% achieved target concentrations with a dose of 6 mg/kg/day (max. 300 mg/day). As expected, the IV formulation had high attainment of prophylaxis targets (81%–90%) with 6–10 mg/kg/day (max. 400 mg/day). All formulations were well tolerated, and no relationship between adverse effects and posaconazole concentrations was identified.

Conclusions
The required posaconazole dose in immunocompromised children varies depending on the formulation. The IV infusion had the highest attainment of therapeutic concentration followed by the DRT and powder for suspension. By contrast, the oral suspension had low attainment of target concentrations despite higher daily doses.