bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depression and anxiety in Chinese patients hospitalized with primary headache: A cross-sectional multicenter study
Authors: Jia Zhihua , Wan Dongjun , Yin Ziming , Fan Zhiliang , Xu Peng , Yuan Xueqian , Chen Min , Wang Dan , Wang Hebo , Wang Shengshu , Zhang Shuhua , Liu Ruozhuo , Wang Xiaolin , Wang Rongfei , Su Hui , Han Xun , Yu Zhe , Li Yingji , Yu Shengyuan , Dong Zhao 
Published: February 2025

Background: Primary headache and psychiatric diseases are bidirectional correlated. The real-world data of depression and anxiety in Chinese patients hospitalized for primary headache, considering all subtypes, remain unclear. Methods: This study enrolled patients attending eight Chinese headache centers from October 2022 to September 2023. A WeChat mini-program was designed to collect data. Headache was diagnosed and confirmed by two headache specialists. The Patient Health Questionnaire-9 and Generalized Anxiety Disorder-7 were used to assess depression and anxiety. Results: Overall, 1963 patients with primary headache were analyzed; the prevalence of depression and anxiety was 20.1% (396/1963) and 14.8% (290/1963), respectively. Of the 1963 patients, 217 (11.1%) had history of anxiety or depression and 184 (9.4%) had undergone assessments. Patients with both primary headache and depression were more likely to be women (77.8% vs 71.9%), experience more severe headache (numerical rating scale; 6.2 ± 1.9 vs 5.7 ± 1.9) and greater impacts on quality of life (Headache Impact Test-6; 65.3± 8.5 vs 58.1 ± 11.5). Those with both primary headache and anxiety exhibited similar results and were less educated. Depression and anxiety were more prevalent in chronic migraineurs (CM) than in episodic migraineurs (36.8% vs 16.9% and 28.9% vs 12.3%, respectively) and in those with chronic (CTTH) than in those with episodic tension-type headache (30.6% vs 15.1% and 20.1% vs 12.8%, respectively). Conclusion: Depression and anxiety are inadequately diagnosed and strongly associated with sex, severe headache, chronification and disability in patients with primary headache in China. To improve the health of patients with primary headaches, early screening for depression and anxiety is important.
Gastrointestinal side effects in hepatocellular carcinoma patients receiving transarterial chemoembolization: a meta-analysis of 81 studies and 9495 patients
Authors: Arendt Nathalie , Kopsida Maria , Khaled Jaafar , Sjöblom Markus , Heindryckx Femke 
Published: February 2025

Background: Transarterial chemoembolization (TACE) is a widely used treatment for hepatocellular carcinoma (HCC), combining targeted chemotherapy and embolization. While effective, TACE can be associated with significant gastrointestinal (GI) side effects, impacting a patient’s quality of life. Objectives: Quantify the prevalence of key GI complications (diarrhea, nausea, GI toxicity, abdominal pain) following TACE. Design: Systematic review was performed following Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines, focusing on studies that reported side effects of TACE. Studies not involving cTACE or drug-eluting bead TACE (DEB-TACE), non-HCC studies, meta-analyses or systematic reviews, and inaccessible publications were excluded. Data sources and methods: A PubMed search for clinical and randomized trials was conducted. Extracted data included study identifiers, demographics, TACE details, and GI side effect prevalences. The Mixed Methods Appraisal Tool assessed study quality and bias. Results: The analysis included data from 81 studies with 121 individual study arms and 9495 patients. Diarrhea was reported in 38 studies, with a mean prevalence of 23.46% (2.5; 95% confidence interval (CI): 18.39–28.544) and a weighted prevalence of 23.5%. Nausea was most frequently reported, mentioned in 67 studies, with a mean prevalence of 34.66% (2.4; 95% CI: 29.89–39.44) and a weighted prevalence of 32.5%. Abdominal pain was reported in 59 studies, with the highest mean prevalence of 48.07% (2.9; 95% CI: 42.20–53.93) and a weighted prevalence of 46.1%. GI toxicity was reported in 32 studies, with a mean prevalence of 8.85% (1.4; 95% CI: 5.99–11.70) and a weighted prevalence of 9.9%. DEB-TACE generally led to slightly higher rates of nausea, diarrhea, abdominal pain, and GI toxicity compared to conventional TACE. The type of chemotherapy agent influenced prevalence of GI-side effects, with high prevalences observed for agents such as zinostatin and cisplatin. Conclusion: This meta-analysis synthesizes current evidence on managing GI side effects in TACE. Standardizing reporting and developing effective management strategies are crucial to improving patient outcomes.
Diabetes Attitudes, Wishes and Needs in the Dominican Republic (DR-DAWN2)
Authors: Soto Manuel , García Nadja , Ortiz Jessica , Calderón Berniza 
Published: February 2025

Purpose The purpose of this study was to assess the psychosocial status of people with diabetes and their perceived quality of received health care services in a local outpatient center in Santo Domingo, Dominican Republic. Methods Cross-sectional study that included 385 Dominican adults with diabetes. Demographic and clinical information was collected. Key psychometric indicators were collected by using the WHO-5 Well-Being Index, EuroQol-5D Visual Analogue Scale (EQ-VAS), Problem Areas in Diabetes Scale, Summary of Diabetes Self-Care Activities, and Patient Assessment of Chronic Illness Care. Results The mean EQ-VAS score was 77.6 (SD 18). Of all the participants, 18.7% exhibited symptoms of depression, with higher rates among women and younger age groups. Diabetes-related distress was present in 22.6% of participants, particularly among younger individuals and those employed. Physical activity was the self-care activity with the lowest reported adherence. Perceptions of received health care services aligned moderately with the chronic care model (CCM), with a higher perceived alignment in patients that were enrolled in a chronic disease management program. Conclusions Patients with diabetes report having a reduced quality of life and significant psychological burdens, with a high rate of depressive symptoms and diabetes-related distress and a low adherence to self-care behaviors. The enrollment in chronic disease management programs improved patients’ perception of received health care services, reporting a more coordinated care and experiencing more patient activation, which could improve health outcomes. These findings underline the need for psychological screening, patient-centered care, and broader implementation of the CCM to improve health outcomes in this population.
Validation of the Food Safe Zone questionnaire for families of individuals with Prader-Willi syndrome
Authors: Dykens Elisabeth M. , Roof Elizabeth , Hunt-Hawkins Hailee , Strong Theresa V. 
Published: February 2025

Abstract

Background
Prader-Willi syndrome (PWS), a genetic neurodevelopmental disorder, is characterized by hyperphagia and significant behavioral problems. Hyperphagic individuals with PWS are chronically hungry yet rarely feel sated, and often engage in food-seeking behaviors. To avoid life-threatening obesity in their children, families implement food security strategies (e.g., locking food sources, constant supervision around food, alerting others). Although widely used, these strategies have yet to be systematically examined. We thus developed and analyzed the psychometric properties of a new measure of these diverse strategies, the Food Safe Zone, and evaluated them in relation to hyperphagic symptoms and demographic variables. In doing so, we also shine a light on the extraordinary efforts of families in managing their children’s hyperphagia.

Methods
Our team developed 20 FSZ items that were revised for clarity and completeness in an iterative feedback process with stakeholders, including parents, PWS specialists, and individuals with PWS. The FSZ was pilot tested, descriptive findings were reviewed by additional stakeholders, and then administered to 624 parents in a large-scale study. Based on an open-ended question, “Is there anything else you do to ensure food safety?” two additional items were added and evaluated in a follow-up study.

Results
Principal component analyses revealed that 21 FSZ items loaded onto 5 factors that were readily interpretable, accounting for 67% of test variance: Alerting Others and Food Supervision in the Community; Locking or Restricting Food Sources; Checking for Food; At Home Supervision and Meals; and Avoiding Food Settings. Internal consistency and test-rest reliability were robust. Convergent validity analyses revealed that parents implemented FSZ strategies in response to the severity of their child’s hyperphagia, and not their child’s age, gender or PWS genetic subtype.

Conclusions
The psychometrically sound FSZ holds promise for future research, especially on the effects of food safety tactics on family members. In future clinical trials, the FSZ could also be used to help parents think critically about their food safety tactics in relation to their child’s hyperphagia, or as an exploratory endpoint; if hyperphagia is lessened, so too may food safety tactics, thereby enhancing familial quality of life.
TockyPrep: data preprocessing methods for flow cytometric fluorescent timer analysis
Authors: Ono Masahiro 
Published: February 2025

Abstract

Background:
Fluorescent Timer proteins, which display time-dependent changes in their emission spectra, are invaluable for analyzing the temporal dynamics of cellular events at the single-cell level. We previously developed the Timer-of-cell-kinetics-and-activity (Tocky) tools, utilizing a specific Timer protein, Fast-FT, to monitor temporal changes in cellular activities. Despite their potential, the analysis of Timer fluorescence in flow cytometry is frequently compromised by variability in instrument settings and the absence of standardized preprocessing methods. The development and implementation of effective data preprocessing methods remain to be achieved.

Results:
In this study, we introduce the R package that automates the data preprocessing of Timer fluorescence data from flow cytometry experiments for quantitative analysis at single-cell level. Our aim is to standardize Timer data analysis to enhance reproducibility and accuracy across different experimental setups. The package includes a trigonometric transformation method to elucidate the dynamics of Fluorescent Timer proteins. We have identified the normalization of immature and mature Timer fluorescence data as essential for robust analysis, clarifying how this normalization affects the analysis of Timer maturation. These preprocessing methods are all encapsulated within the package.

Conclusions:
is available for distribution via GitHub at https://github.com/MonoTockyLab/TockyPrep, providing tools for data preprocessing and basic visualization of Timer fluorescence data. This toolkit is expected to enhance the utility of experimental systems utilizing Fluorescent Timer proteins, including the Tocky tools.
Adverse childhood experiences screening in pediatric primary care and changes in the rate of visits to social work and behavioral health
Authors: Negriff Sonya , Sidell Margo , Barton Lee , DiGangi Mercie J. 
Published: February 2025

Abstract

Background
There is increasing interest in screening for adverse childhood experiences in pediatric primary care, but no evidence of the actual consequences on behavioral/mental health services. This study tested the association between initiation of ACEs screening in pediatric primary care and changes in the rate of referrals to social work and visits to social work and behavioral health.

Methods
Data came from the electronic health records of children and adolescents between 2 and 18 years old who were members of a large integrated healthcare system serving Southern California (N = 513,812). Poisson regression was used to compare the rate of referrals and visits to social work and behavioral health visits for clinics doing standardized ACEs screening (i.e., intervention clinics; n = 28) versus clinics not screening (i.e., control clinics; n = 64) during June 1-December 31 2022 as well as for these same months in 2020 and 2021.

Results
Intervention clinics had an average screening rate of 57% (range 26.8 to 91.9%) and an average positive screen rate of 11% (range 1.6–25.1%). The difference in the adjusted rate from 2021 to 2022 was significantly different between intervention and control clinics for referrals to social work (RR 1.48, 95% CI 1.25, 1.74), but was not statistically different for visits to social work or behavioral health.

Conclusions
The findings suggest that ACEs screening does not significantly increase the rates of social work and behavioral health visits, although it did increase referrals to social work. We acknowledge that this may vary based on geographic areas and populations served by different healthcare systems.
A novel therapeutic approach targeting PD-L1 in HNSCC and bone marrow-derived mesenchymal stem cells hampers pro-metastatic features in vitro: perspectives for blocking tumor-stroma communication and signaling
Authors: Ferrara Ylenia , Latino Debora , Costagliola di Polidoro Angela , Oliver Angela , Sarnella Annachiara , Caprio Maria Grazia , Cerchia Laura , Ruvo Menotti , Sandomenico Annamaria , Zannetti Antonella 
Published: February 2025

Abstract

Background
Current conventional treatment regimens for head and neck squamous cell carcinoma (HNSCC), are poorly effective because of the emergence of resistance mechanisms. Many studies have reported how the tumor microenvironment influences tumor response to immune checkpoint inhibitors targeting PD-1/PD-L1. It has been reported that overexpression of PD-L1 correlates with and is involved in cancer progression by promoting epithelial-to-mesenchymal-transition (EMT) program, stemness and tumor cell invasiveness through AKT and MAPK pathways. In this study, we investigated how bone marrow mesenchymal stem cells (BM-MSCs) recruited and educated by HNSCC cells are able to promote tumor cell invasion and EMT program. In addition, we analyzed how the crosstalk between stromal cells and tumor cells can affect PD-L1 expression levels. In this context, we developed and characterized a novel anti-PD-L1 recombinant Fab (rFab’) and tested its ability to potentiate the effect of cisplatin.

Methods
BM-MSCs and HNSCC cells co-cultures, cell migration and invasion were performed using Boyden chambers. The effect of treatments on cell viability and growth were analyzed by MTT and clonogenic assay, respectively. The anti-PD-L1 rFab’ was prepared in E. Coli and tested for its binding on HNSCC cells and BM-MSCs by FACS analysis and fluorescence microscopy. PD-L1, p-AKT, p-ERK, N-cadherin and β-catenin expression levels were analyzed by western blotting.

Results
BM-MSCs were induced by tumor cells to migrate, invade and to trans-differentiate in cancer associated fibroblasts (CAFs) as demonstrated by increased expression levels of α-SMA and FAP-α. BM-MSCs contributed to HNSCC invasiveness by increasing p-AKT, p-ERK, N-cadherin and β-catenin expression levels. When BM-MSCs and HNSCC cells were co-cultured the level of PD-L1 expression was enhanced in both cells indicating a reciprocal support in favoring tumor aggressiveness. Tumor cell treatment with rFab’ anti-PD-L1 reduced their viability, growth, migration and invasion and blunted the underlying signaling pathways. In addition, rFab’ anti-PD-L1 was able to potentiate the antitumor effect of cisplatin on HNSCC cells.

Conclusions
BM-MSCs recruited and educated by HNSCC cells support tumor cell aggressiveness via PD-L1. A novel rFab’ anti-PD-L1 reduces HNSCC proliferation, migration and invasion and potentiates the cisplatin effect suggesting its potential to be conjugated with drugs for immuno-cytotoxic therapy.
Promoting the transition from pyroptosis to apoptosis in endothelial cells: a novel approach to alleviate methylglyoxal-induced vascular damage
Authors: Yuan Ruqiang , Xu Hu , Wang Mingqi , Guo Lina , Yao Yang , Zhang Xiaoru , Wang Xiuli 
Published: February 2025

Abstract

Background
Methylglyoxal (MGO)-induced cell death in vascular endothelial cells (VECs) plays a critical role in the progression of diabetic vascular complications (DVCs). Previous studies have shown that MGO can induce inflammatory pyroptosis, leading to VEC damage. However, the underlying mechanism remains unclear, and effective interventions are yet to be developed.

Methods
Human umbilical vein endothelial cells (HUVECs) were used for in vitro experiments. Cell death modes were assessed through morphological observations. Mechanistic investigations were performed using immunofluorescence, flow cytometry, Western blotting, and ELISA. Inhibitors and adenoviruses were employed to validate the mechanisms. Vascular organoids in conjunction with AngioTool plug-in assays were used to evaluate VEC damage and angiogenic capacity. Mouse blood pressure was measured using the tail-cuff method, and vascular morphology was examined through hematoxylin and eosin (H&E) staining as well as immunofluorescence staining. Data were analyzed using the GraphPad Prism software.

Results
Our study revealed that MGO induces pyroptosis in VECs via the Caspase3/gasdermin E (GSDME) pathway. Furthermore, the saponin monomer 13 of dwarf lilyturf tuber (DT-13), inhibited MGO-induced pyroptosis and promoted the generation of apoptotic bodies, facilitating the transition from pyroptosis to apoptosis. Mechanistically, DT-13 suppressed the Caspase3-mediated cleavage of GSDME and non-muscle myosin heavy chain IIA (NMMHC IIA), while increasing the phosphorylation of myosin light chain 2 (MLC2), which facilitated apoptotic body formation. Additionally, DT-13 was shown to mitigate VEC damage, inhibit angiogenesis, reduce vascular remodeling, and alleviate MGO-induced hypertension.

Conclusions
This study uncovers a novel mechanism through which MGO induces VEC damage, highlighting the therapeutic significance of the transition from pyroptosis to apoptosis in this process. These findings suggest potential therapeutic strategies for managing diabetic angiopathy. Furthermore, DT-13 emerges as a promising compound for therapeutic intervention, offering new possibilities for clinical applications.
Cardiac manifestations in adult MELAS syndrome (mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome)– a cross-sectional study
Authors: Stoevesandt Dietrich , Schlitt Axel , Röntgen Philipp , Kraya Torsten 
Published: February 2025

Abstract

Backround
Cardiac involvement has been reported in different mitochondrial geno- and phenotypes, including mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like (MELAS) syndrome. However, cardiac manifestations are diverse and not well described.

Methods
We prospectively examined cardiac manifestations in 11 adult patients with MELAS syndrome harboring the MTTL1 m.3243A < G-mutation using patient records, cardiac MRI (1.5 Tesla), echocardiography, electrocardiogram (ECG), laboratory tests of cardiac markers (CK, CK-MB, Trop I, BNP), and clinical severity (NMDAS = Newcastle Mitochondrial Disease Scale).

Results
Among 11 consecutive patients with MELAS syndrome (73% male, mean age 37.5 ± 10.6 years) cardiac manifestations were found in nine (82%). Pathology was mainly detected using MRI (9 of 11, 82%). Six patients showed diffuse late enhancement in the left ventricle, one a left ventricular ejection fraction (LVEF) below 30%, two with a LVEF in the range of 40–50% in the cardiac MRI, and another five patients presenting diastolic dysfunction as defined by echocardiography. Only one patient with late enhancement on MRI also showed a conduction block in the ECG. There was no correlation between the cardiac manifestations and the NMDAS score or heteroplasmy grade.

Conclusions
Cardiac involvement in MELAS syndrome harboring the MTTL1 m.3243A > G mutation mostly entails cardiomyopathy, which was particularly evident in the cardiac MRI. Only one patient (1/11, 9.1%) had conduction defects. Thus, cardiac testing including cardiac MRI, echocardiography and ECG seems to be important for prognosis of MELAS patients.
Ecofriendly colorimetric set-up coupled with mathematical filtration strategy for simultaneous determination of ipratropium and fenoterol in their novel anti-asthmatic metered dose inhaler
Authors: Ali Salma N. , Marzouk Hoda M. , Fayed Ahmed S. , Saad Samah S. 
Published: February 2025

Abstract
Ipratropium bromide (IPR) and fenoterol hydrobromide (FEN) are well-known medications for treating asthma and chronic obstructive pulmonary disease (COPD). A simple, feasible, efficient, and cost-effective colorimetric assay has been established for determination of the newly introduced co-formulated metered dose inhaler (Atrovent® comp HFA). The developed method is based on the properly optimized reaction of drugs under study with the charge transfer reagent 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), resulting in orange-colored complexes measured at 464.3 and 514.0 nm for IPR and FEN, respectively. Smoothly applicable and easily manipulated resolution method, Vierordt’s method, based on straightforward mathematical equations that do not need complicated software was implemented for the simultaneous determination of IPR and FEN. The proposed methodology can be applied in routine analysis enabling fast and economical determination of the combined dosage form without the need for previous separation steps. The validity of the proposed colorimetric method was thoroughly assured as per ICH guidelines with acceptable accuracy and precision. The linearity ranges for IPR and FEN were 5.0–55.0 µg/mL and 10.0–40.0 µg/mL, respectively. Using cutting-edge software metric tools, namely the analytical greenness (AGREE), and complementary green analytical procedure index (ComplexGAPI), the greenness profile of the suggested method was clearly evaluated. The method also conformed well to the recently published blueness (BAGI tool) and whiteness (RGB12 tool) concepts.
Biphasic anaphylaxis in a Canadian tertiary care centre: an evaluation of incidence and risk factors from electronic health records and telephone interviews
Authors: Ellis Anne K. , Hossenbaccus Lubnaa , Linton Sophia , Botting Hannah , Badawod Eman , Burrows Alyssa , Garvey Sarah 
Published: February 2025

Abstract

Background
Our previous 2007 study reported a 19.4% rate of biphasic anaphylaxis in Kingston, Ontario. Since then, few updates have been published regarding the etiology and risk factors of biphasic anaphylaxis. This study aimed to describe the incidence of and predictors of biphasic anaphylaxis in a single centre through a retrospective evaluation of patients with diagnosed anaphylaxis.

Methods
From November 2015 to August 2017, all patients who presented to the emergency department at two hospital sites in Kingston given a diagnosis of “allergic reaction,” “anaphylaxis,” “drug allergy,” or “insect sting allergy,” were evaluated. Patients were contacted sometime after ED discharge to obtain consent and confirm symptoms and timing of the reaction. A trained allergist determined if criteria for anaphylaxis were met and categorized the reactions as being uniphasic, biphasic, or non-anaphylactic biphasic. A full medical review of the event ensued, and each type of anaphylactic event was statistically compared.

Results
Of 138 anaphylactic events identified, 15.94% were biphasic reactions, 79.0% were uniphasic, and 5.07% were classified alternatively as a non-anaphylactic biphasic reaction. The average time of a second reaction was 19.0 h in patients experiencing biphasic reactivity. For biphasic anaphylaxis, the symptom profiles of second reactions were significantly less severe (p = 0.0002) compared with the initial reaction but significantly more severe than non-anaphylactic biphasic events (p < 0.0001).No differences of management were identified between events.

Conclusion
The incidence of biphasic reactions in this cohort was 15.94% and the average second-phase onset was 19.0 h. In biphasic reactivity, it appears that the symptom profile second reaction is less severe compared to the first reaction.