bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective function ofex vivoexpanded CD8 T cells in a mouse model of adoptive therapy for cytomegalovirus infection depends on integrin beta 1 but not CXCR3, CTLA4, or PD-1 expression
Authors: Liu Xiaokun , Jauregui Rodrigo Gutierrez , Lueder Yvonne , Halle Stephan , Ospina-Quintero Laura , Ritter Christiane , Schimrock Anja , Willenzon Stefanie , Janssen Anika , Wagner Karen , Messerle Martin , Bošnjak Berislav , Förster Reinhold 
Published: March 2024

AbstractThe adoptive transfer of virus-specific T cells (VSTs) represents a therapeutic option for viral infection treatment in immunocompromised patients. Before administration,ex vivoculture enables VST expansion. However, it is unclear howex vivoexpansion affects the circulation, homing, and intra-tissue migration of administered VSTs. We established a model of VST immunotherapy of acute cytomegalovirus infection using adoptive transfer ofex vivoexpanded OT-I CD8 T cells (recognizing SIINFEKL peptide) intoRag2-/-mice infected with murine cytomegalovirus (MCMV) encoding for the SIINFEKL peptide.Ex vivoexpansion induced an effector T cell phenotype and affected the expression of integrins and chemokine receptors. CRISPR/Cas9-mediated gene deletions enabled us to address the role of selected genes in the homing of VSTs following intravenous administration. We found that deletion ofItgb1, encoding for integrin beta 1, prevented OT-I cells from entering infected organs and drastically reduced their number in blood, suggesting that adoptively transferred VSTs primarily expand in the infected tissues. In contrast,Cxcr3-/-OT-I cells provided equal protection as theirCxcr3+/+counterparts, indicating that this chemokine receptor does not contribute to VST entry into infected organs. Further,Pdcd1andCtla4deletion did not impair the transferred OT-I cells’ ability to protect mice from MCMV, arguing against quick exhaustion of VSTs with an effector T cell phenotype. Together, these data indicate thatex vivoexpansion affects migration and activation properties of VSTs and suggest that future clinical evaluation of adoptive T cell therapy efficacy should include homing molecule expression assessment.
Suppressive effects of oroxylin A on intracellular proliferation ofToxoplasma gondiivia host cell ERK phosphorylation inhibition
Authors: Zhang Ziyue Z , Norose Kazumi , Shinjyo Noriko , Lin Xiaoxia X , Suganami Akiko , Tamura Yutaka , Sakamoto Hirokazu , Hikosaka Kenji 
Published: March 2024

ABSTRACTToxoplasma gondiiposes a significant threat to immunocompromised patients, resulting in high mortality rates. Considering the side effects of anti-Toxoplasmadrugs, we focused on a potential candidate, oroxylin A (OA), a common component extracted fromAstragalus membranaceusandScutellaria baicalensiswhich suppressed the growth ofT. gondii in vitroandin vivo.Our result demonstrated that OA suppressedT. gondiiintracellular proliferation and downregulated phosphorylation of ERK1/2 ofT. gondii-infected host cells very similar to the MEK1 specific inhibitor PD98059. Ourin silicoanalysis showed that OA interacts sufficiently with the mammalian MEK1 region where established MEK1 inhibitors like PD98059 and trametinib bind. Moreover, OA improved the survival rate inT. gondii-infected mice. This study proposes that host MEK1 is a novel potential target for anti-Toxoplasmadrugs with a new mechanism of action.
Cardiovascular disease risk score derivation and validation in Abu Dhabi, United Arab Emirates. Retrospective Cohort Study
Authors: Baynouna AlKetbi Latifa , Nagelkerke Nico , Humaid Ahmed , AlAlawi Noura , AlKetbi Rudina , Aleissaee Hamda , AlShamsi Noura , Abdulbaqi Hanan , Fahmawee Toqa , AlHashaikeh Basil , AlDobaee Muna , AlShamsi Mariam , AlAhbabi Nayla , AlAzeezi AlYazia , Shuaib Fatima , Alnuaimi Jawaher , Mahmoud Esraa , AlDhaheri Alreem , AlMansoori Mohammed , AlKalbani Sanaa , AlDerie Wesayef , Saeed Ekram , AlMarzooqi Nouf , AlHassani Ahmed , AlAhmadi Amira , Sahyoni Mohammed , AlFahmawi Farah , AlAlawi Ali , Sahalu Yusra , AYahyaee Aysha , AlAnsari Zinab , Doucoure Khadija , Ashoor Rawan , AlShamsi Reem , AlAzeezi Maha , AlMeqbaali Fatima , Yahya Noor , AlAlawi Shamma , AlKetbi Fatima 
Published: March 2024

AbstractCardiovascular disease (CVD) risk assessment is key to rational decision-making in primary prevention. The CVD risk depends on dynamic factors requiring continuous equation updates.DesignThe Abu Dhabi Risk Study (ADRS) is the first and longest-duration retrospective cohort study in Abu Dhabi and the United Arab Emirates (UAE), aiming to develop 10-year risk prediction equations for CAD, stroke, and ASCVD (Atherosclerotic Cardiovascular Disease, combining CAD and stroke) and validate international risk equations.MethodThe included 8699 subjects are participants of the national cardiovascular screening program of 2011-2013 with an average follow-up of 9.2 years. They were assessed retrospectively in 2023 for health outcomes. The validation cohort, 2554 subjects, is another community-based screening program done in Abu Dhabi in the period from 2016 and 2017. With an average follow-up of 6.67 years.ResultsOf 8504 who were ASCVD-free, 250 had new coronary artery events. Identified risk factors for ASCVD in this population were the conventional risk factors such as age, gender, smoking, high cholesterol/HDL ratio, and diabetes diagnosis, in addition to low vitamin D level, and low glomerular filtration rate (GFR) levels.Three ADRS prediction models were derived from Cox regression. The ADRS-CAD had a C- statistic of 0.899 compared to 0.828 of FRS (Framingham score) in the same population.ADRS-Stroke had a c-statistic of 0.904. The ADRS-ASCVD had a c-statistic of 0.898 compared to 0.891 of PCE (pooled cohort equations) and 0.825 of FRS-CVD.Applying the developed formulas to the validation cohort showed good predictability of CAD and ASCVD events with an ASCVD c-statistic of 0.825, for CAD the c-statistic was 0.799, and for stroke, it was 0.761. The PCE showed similar performance in this cohort with a c-statistic for ASCVD of 0.824.ConclusionThis study demonstrated the value of tailoring risk assessments to local populations and healthcare contexts.
Effects of context changes on memory reactivation
Authors: Özdemir Şahcan , Şentürk Yağmur Damla , Ünver Nursima , Demircan Can , Olivers Christian N.L. , Egner Tobias , Günseli Eren 
Published: March 2024

AbstractWhile the influence of context on long-term memory (LTM) is well-documented, its effects on the interaction between working memory (WM) and LTM remain less understood. In this study, we explored these interactions using a delayed match-to-sample task, where participants (6 Male, 16 Female) encountered the same target object across six consecutive trials, facilitating the transition from WM to LTM. During half of these target repetitions, the background color changed. We measured the WM storage of the target using the contralateral delay activity (CDA) in electroencephalography (EEG). Our results reveal that task-irrelevant context changes trigger the reactivation of long-term memories in WM. This reactivation may be attributed to content-context binding in WM and hippocampal pattern separation.Significance StatementUnderstanding the mechanisms of memory updating in response to changing contexts is vital because context plays a pivotal role in shaping long-term memories. This study demonstrates, for the first time, that an irrelevant context change triggers the reactivation of learned memories in visual working memory. This observation underscores the importance of multi-memory interactions during context updating. Challenging traditional memory models that postulate mandatory reactivation of long-term memories upon each use, our results instead reveal a selective reactivation process, especially during transitions to new environments. This finding elucidates the adaptive nature of memories and enhances our understanding of memory storage and retrieval processes.
Convergent effects of different anesthetics on changes in phase alignment of cortical oscillations
Authors: Bardon Alexandra G. , Ballesteros Jesus J. , Brincat Scott L. , Roy Jefferson E. , Mahnke Meredith K. , Ishizawa Yumiko , Brown Emery N. , Miller Earl K. 
Published: March 2024

AbstractMany anesthetics cause loss of responsiveness despite having diverse underlying molecular and circuit actions. To explore the convergent effects of these drugs, we examined how anesthetic doses of ketamine and dexmedetomidine affected oscillations in the prefrontal cortex of nonhuman primates. Both anesthetics caused increases in phase locking in the ventrolateral and dorsolateral prefrontal cortex, within and across hemispheres. However, the nature of the phase locking varied. Activity in different subregions within a hemisphere became more anti-phase with both drugs. Local analyses within a region suggested that this finding could be explained by broad cortical distance-based effects, such as large traveling waves. By contrast, homologous areas across hemispheres became more in-phase. Our results suggest that both anesthetics induce strong patterns of cortical phase alignment that are markedly different from those in the awake state, and that these patterns may be a common feature driving loss of responsiveness from different anesthetic drugs.
Crystal structure solution and high-temperature thermal expansion in NaZr2(PO4)3-type materials
Authors: Hulbert Benjamin S. , Brodecki Julia E. , Kriven Waltraud M. 
Published: March 2024

The NaZr2P3O12 family of materials have shown low and tailorable thermal expansion properties. In this study, SrZr4P6O24 (SrO·4ZrO2·3P2O5), CaZr4P6O24 (CaO·4ZrO2·3P2O5), MgZr4P6O24 (MgO·4ZrO2·3P2O5), NaTi2P3O12 [½(Na2O·4TiO2·3P2O5)], NaZr2P3O12 [½(Na2O·4ZrO2·3P2O5)], and related solid solutions were synthesized using the organic–inorganic steric entrapment method. The samples were characterized by in-situ high-temperature X-ray diffraction from 25 to 1500°C at the Advanced Photon Source and National Synchrotron Light Source II. The average linear thermal expansion of SrZr4P6O24 and CaZr4P6O24 was between −1 × 10−6 per °C and 6 × 10−6 per °C from 25 to 1500°C. The crystal structures of the high-temperature polymorphs of CaZr4P6O24 and SrZr4P6O24 with R
3
c symmetry were solved by Fourier difference mapping and Rietveld refinement. This polymorph is present above ∼1250°C. This work measured thermal expansion coefficients to 1500°C for all samples and investigated the differences in thermal expansion mechanisms between polymorphs and between compositions.
Theory of environmentally responsible behavior (TERB) to predict waste reduction behaviors among young coastal tourists: a case of Bangladesh
Authors: Hasan Abdulla Al-Towfiq , Aziz Rukaya 
Published: March 2024

Purpose
The purpose of this paper is to develop the theory of environmentally responsible behavior by extending the theory of planned behavior to empirically examine waste reduction behaviors among young coastal tourists in Bangladesh.

Design/methodology/approach
Through review of literature, in-depth interviews and survey, the study is conducted. Initial survey collects 355 data, which is used for exploratory factor analysis to develop constructs and its measurement items. After preparing the formal survey questionnaires, another survey is conducted and collected 403 usable responses. The subsequent valid responses are used for confirmatory factor analysis through structural equation modeling, using Smart PLS 3.3.3.

Findings
The results of this study reveal that waste reduction behavior is significantly affected by waste reduction intention, and then subsequently waste reduction intention is significantly affected by environmental concern, climate change concern and healthy environmental norms of young coastal tourists in Bangladesh.

Practical implications
The findings of the study will benefit industry operators and policymakers by understanding the factors that are crucial for influencing young costal tourists’ environmentally responsible behaviors (i.e. waste reduction behaviors). Eventually, the findings may assist industry operators and policymakers to develop strategies to attract more young tourists to the coastal tourist destinations in Bangladesh.

Originality/value
The importance of environmentally responsible behavior has received a significant attention in present decade. Consistent with this phenomenon, this study uniquely develops the theory of environmentally responsible behavior and establishes the link between environmental concern, climate change concern, healthy environmental norms, waste reduction intention and waste reduction behavior in coastal tourism domain in Bangladesh.
Greenhouse gas emissions patterns and insertion in global value chains: a comparative study between Brazil and China (2000–2016)
Authors: Barcelos Tiago Ferreira , Costa Kaio Glauber Vital 
Published: March 2024

PurposeThis study aims to analyze and compare the relationship between international trade in global value chains (GVC) and greenhouse gas (GHG) emissions for Brazil and China from 2000 to 2016.Design/methodology/approachThe input-output method apply to multiregional tables from Eora-26 to decompose the GHG emissions of the Brazilian and Chinese productive structure.FindingsThe data reveals that Chinese production and consumption emissions are associated with power generation and energy-intensive industries, a significant concern among national and international policymakers. For Brazil, the largest territorial emissions captured by the metrics come from services and traditional industry, which reveals room for improving energy efficiency. The analysis sought to emphasize how the productive structure and dynamics of international trade have repercussions on the environmental dimension, to promote arguments that guide the execution of a more sustainable, productive and commercial development strategy and offer inputs to advance discussions on the attribution of climate responsibility.Research limitations/implicationsThe metrics did not capture emissions related to land use and deforestation, which are representative of Brazilian emissions.Originality/valueComparative analysis of emissions embodied in traditional sectoral trade flows and GVC, on backward and forward sides, for developing countries with the main economic regions of the world.
Exploring lean manufacturing drivers for enhancing circular economy performance in the pharmaceutical industry: a Bayesian best–worst approach
Authors: Salman Sheak , Morshed Shah Murtoza , Karim Md. Rezaul , Rahman Rafat , Hasanat Sadia , Ahsan Afia 
Published: March 2024

PurposeThe imperative to conserve resources and minimize operational expenses has spurred a notable increase in the adoption of lean manufacturing within the context of the circular economy across diverse industries in recent years. However, a notable gap exists in the research landscape, particularly concerning the implementation of lean practices within the pharmaceutical industry to enhance circular economy performance. Addressing this void, this study endeavors to identify and prioritize the pivotal drivers influencing lean manufacturing within the pharmaceutical sector.FindingsThe outcome of this rigorous examination highlights that “Continuous Monitoring Process for Sustainable Lean Implementation,” “Management Involvement for Sustainable Implementation” and “Training and Education” emerge as the most consequential drivers. These factors are deemed crucial for augmenting circular economy performance, underscoring the significance of management engagement, training initiatives and a continuous monitoring process in fostering a closed-loop practice within the pharmaceutical industry.Research limitations/implicationsThe findings contribute valuable insights for decision-makers aiming to adopt lean practices within a circular economy framework. Specifically, by streamlining the process of developing a robust action plan tailored to the unique needs of the pharmaceutical sector, our study provides actionable guidance for enhancing overall sustainability in the manufacturing processes.Originality/valueThis study represents one of the initial efforts to systematically identify and assess the drivers to LM implementation within the pharmaceutical industry, contributing to the emerging body of knowledge in this area.
Fostering entrepreneurship and development in rural mountainous regions: the role of SEZs and local economic dynamics in Gilgit-Baltistan
Authors: Batool Sajida , Baig Saranjam , Khalid Mehmood , Alam Khalid Mehmood 
Published: March 2024

PurposeThis study aims to examine the perceptions and opinions of relevant stakeholders regarding entrepreneurship opportunities and growth in the Gilgit-Baltistan (GB) province of Pakistan. Specifically, it focuses on the role of special economic zones (SEZs), such as Maqpondass SEZ and the China–Pakistan Economic Corridor (CPEC), in fostering nascent entrepreneurship (NE) and promoting regional development.Design/methodology/approachThe study employs ordered logistic regression to estimate the relationship between various independent variables and nascent entrepreneurship (NE). The independent variables include awareness of CPEC (AAC), awareness of Maqpondass SEZ (AAMEZ), SEZ incentives (SEZInc), regional market competitiveness (RMC), loan availability (LA) and education and experience (EE).FindingsThe findings indicate a robust positive relationship between SEZ-based industries and the growth of local small businesses and enterprises in Gilgit-Baltistan. Furthermore, the study suggests that government incentives, access to finance, skill development, relevant knowledge, and connections with local businesses facilitate the establishment of new ventures.Practical implicationsThe study underscores the importance of focusing on human capital development, providing financial assistance, and creating incentives for adopting advanced technology to foster the growth of local businesses in Gilgit-Baltistan through SEZs. It emphasizes the need for policymakers and stakeholders to prioritize initiatives that support entrepreneurship and innovation in the region.Originality/valueThis study contributes to the existing literature by providing novel insights into the perceptions of entrepreneurship development in Gilgit-Baltistan, particularly concerning the influence of natural resources and SEZs. It fills a gap in the research by offering valuable implications for policymakers, researchers, and practitioners seeking to promote sustainable economic development in the region.
Psychographic characteristics and demand decisions for life insurance products in Uganda
Authors: Ndawula Yusuf Katerega , Neema Mori , Nkote Isaac 
Published: March 2024

PurposeThis study examines the relationship between policyholders’ psychographic characteristics and demand decisions for life insurance products in Uganda.Design/methodology/approachThe study is based on a cross-sectional survey. Using a purposive sampling method, 389 questionnaires were administered to life insurance policyholders in the four geographical regions of Uganda. Partial least squares structural equation modeling (PLS-SEM) was employed to analyze the primary data, specifically to test the relationships between the dependent and independent variables.FindingsThe findings indicate a positive and significant influence of psychographic characteristics on demand decisions for life insurance products. In addition, the analysis indicates that the two first-order constructs of psychographic characteristics, namely price consciousness and consumer innovativeness, are positive and significant predictors of demand decisions for life insurance products. In contrast, the third first-order construct religious salience, exhibits a negative and nonsignificant effect on demand decisions for life insurance products.Practical implicationsFor insurance practitioners, to influence demand decisions, they should emphasize premium-related appeals in their marketing messages (price consciousness) ignore product decisions based on religious beliefs and norms (religious salience). They should also ensure that insurance products are highly trustable and experiential (consumer innovativeness). For insurance policymakers, it offers an in-depth understanding of customer psychographic characteristics, which can be used to identify exploitative information embedded in certain marketing campaigns targeting specific psychographic characteristics, for better regulation.Originality/valueThe study provides a basis for understanding lifestyle and personality characteristics (psychographics), which may influence demand decisions for life insurance products in a developing country like Uganda, where the insurance industry is at an early stage of development.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-06-2023-0440
Caught in the web: a meta-analysis of Internet addiction, excessive daytime sleepiness and depressive symptoms in adolescents
Authors: Waheed Hassam , Macaulay Peter J.R. , Al-Jaifi Hamdan Amer Ali , Allen Kelly-Ann , She Long 
Published: March 2024

PurposeIn response to growing concerns over the negative consequences of Internet addiction on adolescents’ mental health, coupled with conflicting results in this literature stream, this meta-analysis sought to (1) examine the association between Internet addiction and depressive symptoms in adolescents, (2) examine the moderating role of Internet freedom across countries, and (3) examine the mediating role of excessive daytime sleepiness.Design/methodology/approachIn total, 52 studies were analyzed using robust variance estimation and meta-analytic structural equation modeling.FindingsThere was a significant and moderate association between Internet addiction and depressive symptoms. Furthermore, Internet freedom did not explain heterogeneity in this literature stream before and after controlling for study quality and the percentage of female participants. In support of the displacement hypothesis, this study found that Internet addiction contributes to depressive symptoms through excessive daytime sleepiness (proportion mediated = 17.48%). As the evidence suggests, excessive daytime sleepiness displaces a host of activities beneficial for maintaining mental health. The results were subjected to a battery of robustness checks and the conclusions remain unchanged.Practical implicationsThe results underscore the negative consequences of Internet addiction in adolescents. Addressing this issue would involve interventions that promote sleep hygiene and greater offline engagement with peers to alleviate depressive symptoms.Originality/valueThis study utilizes robust meta-analytic techniques to provide the most comprehensive examination of the association between Internet addiction and depressive symptoms in adolescents. The implications intersect with the shared interests of social scientists, health practitioners, and policy makers.