bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic and epigenetic factors that influence the occurrence of spontaneous lymphoid tumors in crosses of mice of high‐ and low‐incidence strains
Authors: Duran‐Reynals Maria L. , Kadish Anna S. , Lilly Frank 
Published: February 2007

AbstractAKR mice, which spontaneously develop >90% incidence of lymphocytic leukemia (LL), crossed with SJL mice, which show >80% incidence of Hodgkin's‐like reticulum‐cell sarcoma (RCS), produced F1 progeny showing incidences of 30% LL and 0% RCS. Thus, each strain possesses one or more dominant genes capable of interfering with the emergence of the tumor type typical of the other strain. Although mice of reciprocal F1 crosses showed a profound difference in expression of endogenous ecotropic murine leukemia virus (E‐MuLV) due to a maternal resistance factor transmitted by SJL females but not males, the two populations did not differ detectably in LL incidence. Like AKR mice, mice of 5 other strains studied (C58, DBA/2, PL, RF and ST/b) possessed one or more genes conferring resistance to RCS in F1 crosses with SJL. Analysis of LL incidences in F1 generations of all possible crosses among these 7 strains revealed 4 different categories of strains with respect to susceptibility/resistance to LL; only ST/b mice, which show no significant incidence of spontaneous LL, lacked genes that could suppress the disease in crosses with high‐ or moderate‐incidence strains. SJL mice treated topically with 3‐methylcholanthrene (MCA) developed a 50% incidence of LL, mostly before one year of age; treated mice surviving after one year of age developed a high incidence of RCS.
Loss of HLA‐A,B,C and de novo expression of HLA‐D in colorectal cancer
Authors: Momburg Frank , Degener Theodora , Bacchus Elisabeth , Moldenhauer Gerhard , Hämmerling Günter J. , Möller Peter 
Published: February 2007

AbstractOne hundred primary colorectal carcinomas and 19 metastases were studied for the expression of HLA‐A,B,C and HLA‐D antigens using monoclonal antibodies (MAbs) against framework determinants and a sensitive immunoperoxidase technique on frozen sections. With MAb W6/32, 65 tumors were intensely stained for HLA‐A,B,C; 22 showed a reduced staining intensity, in 5 carcinomas a minor or major subset of the tumor cells lacked HLA‐A,B,C, and in 8 cases all tumor cells were unreactive. The loss of HLA‐A,B,C was inversely correlated with the degree of differentiation. No relation was found to type, stage and site of the tumor, or to age and sex of the patients. With MAb 2.06, 58 tumors were HLA‐D negative, 20 exhibited small positive foci, 19 showed a patchy staining pattern, and 3 were homogeneously HLA‐D positive. There was no apparent correlation between HLA‐D expression and any of the clinico‐pathological features mentioned, nor was it related to the loss of HLA‐A,B,C. About half of the metastases displayed the same staining pattern for HLA‐A,B,C and ‐D as the respective primary tumors. The remainder differed from the primary carcinoma by gain or loss of HLA antigens.
Occupational exposure and lung cancer risk
Authors: Kvåle Gunnar , Bjelke Erik , Heuch Ivar 
Published: February 2007

AbstractThe importance of occupation held longest as a risk factor for lung cancer was examined in a prospective study in Norway of 11,995 men, among whom 125 cases occurred in a follow‐up from 1966 through 1978. Based on information about occupation held longest, the respondents were classified into 3 groups according to suspected exposure to respiratory carcinogens at the workplace. After stratification for age, place of residence and cigarette smoking, we found a highly significant relative risk of 2.6 for those judged to have experienced definite exposure versus the group with no workplace exposure. The apparent risk‐enhancing effect of occupational exposure was observed for all histologic subtypes. Stratification including a socioeconomic factor score led to a moderate reduction in the relative risk estimate. High risk estimates still obtained, however, for a limited number of occupations, the highest for workers in the mining and quarrying industries. Although the interpretation of the observed effect associated with a crude index of occupational exposure may be difficult, our results suggest that between 13 and 27% of the lung cancer cases observed among Norwegian men in the relevant time period can be attributed to harmful work‐place exposure.
The Epstein‐Barr virus determined nuclear antigen is composed of at least three different anitgens
Authors: Dillner Joakim , Kallin Bengt , Ehlin‐Henriksson Barbro , Rymo Lars , Henle Werner , Henle Gertrude , Klein George 
Published: February 2007

AbstractThe EBV‐determined nuclear antigen, EBNA, is the only known viral product to be regularly detected in all EBV‐transformed cells. The anticomplement immunofluorescence (ACIF) staining detects an EBV‐specific nuclear reaction that has recently been shown to be due to at least 2 different proteins, EBNA‐1 and EBNA‐2, encoded by different parts of the viral genome. We now report the existence of a third antigen of the EBNA complex, designated as EBNA‐3. Serum from a patient with chronic infectious mononucleosis contained no detectable antibodies to EBNA‐1 and had only a low EBNA‐2 antibody level. Nevertheless, it gave an EBV‐specific nuclear reaction of normal intensity and stained EBNA‐2‐positive and EBNA‐2‐negative EBV‐carrying lines equally well. Immunoblotting with the same serum identified a new EBV‐specific nuclear protein of 143‐157 kDa.
Melanoma in Western Australia 1975–76 TO 1980–81: Trends in demographic and pathological characteristics
Authors: English Dallas R. , Heenan Peter J. , Holman C. D'Arcy J. , Armstrong Bruce K. , Blackwell John B. , Kelsall G. Robert H. , Matz Leonard R. , Singh Amarjeet , Seldam Rolf E. J. Ten 
Published: February 2007

AbstractTrends in incidence rates of cutaneous malignant melanoma in Western Australia from 1975–76 to 1980–1981 have been examined with reference to age, sex, body site, presence or absence of dermal invasion, tumour thickness, histological type, socioeconomic status and occupation. The incidence rates of all melanomas increased from 22.1 to 31.5 per 100,000 person‐years in males and from 23.6 to 28.6 in females. In males, the relative annual increase in the incidence of invasive melanoma was 2.2%, and in females it was 5.6%. In‐situ melanomas had larger relative increases in incidence (28% per annum in males and 10% in females) and the thickness of invasive lesions decreased between 1975–76 and 1980–81. The relative increase in incidence of invasive melanomas was greatest on the body sites with the highest rates initially–the trunk in males and the lower limbs in females. There was an increase in the proportion of invasive lesions classified as superficial spreading melanoma. The increase in incidence of in situ melanomas was largely restricted to the head and neck in older men of high socioeconomic status resident in Perth. This trend in in‐situ melanoma was mainly due to an increase in the recorded incidence of Hutchinson's melanotic freckle. It may have been, in part, an artefact due to increased recognition of Hutchinson's melanotic freckle in this sub‐group of the population.
Studies of cellular retinol‐binding protein (CRBP) in squamous‐cell carcinomas of the head and neck region
Authors: Fex G. , Wahlberg P. , Biörklund A. , Wennerberg J. , Willén R. 
Published: February 2007

AbstractCellular retinol‐binding protein (CRBP) concentration was determined by radioimmunoassay in biopsies from normal mucosa and squamous‐cell cancers of the head and neck region in 26 patients. The plasma concentrations of retinol, retinol‐binding protein (RBP), prealbumin, albumin, orosomucoid and alfa1‐antitrypsin were also determined. In all patients the tumours contained significantly higher concentrations of CRBP (median = 185μg/g) tissue protein; range: 27–1,017μg/g) than normal mucosa (median = 14 μg/g tissue protein; range 6–97 μg/g). CRBP could not be detected in patient plasma. The tumour/normal mucosa CRBP concentration ratio showed a significant inverse correlation to a histopathological malignancy grade score evaluating the tumour‐host relationship, suggesting that tumours with high CRBP relative to normal mucosa are biologically less aggressive. Tumour CRBP showed no correlation either to CRBP concentration in normal mucosa, or to plasma retinol or plasma retinol‐binding protein concentration. CRBP concentration in normal mucosa, however, showed a significant correlation to plasma retinol‐binding protein concentration. Most of the patients had low levels of plasma retinol and retinol‐binding protein compared to matched controls. Whether this has any relationship to the development of the tumour, or whether the active inflammation induced by the cancer leads to a low plasma retinol concentration, is unknown.
Multiple myeloma in Western Australia: A case‐control study in relation to occupation, father's occupation, socioeconomic status and country of birth
Authors: Nandakumar A. , Armstrong Bruce K. , de Klerk Nicholas H. 
Published: February 2007

AbstractA death‐certificate‐based case‐control study was undertaken covering 249 patients with multiple myeloma diagnosed in Western Australia from 1975 to 1984 whose deaths were registered in Western Australia in the same period (73.4% of the total diagnosed) and 996 control decedents who were matched to the cases by age, sex and death registration district. Death from multiple myeloma was associated with both farming [odds ratio (OR) 1.44 with 95% confidence interval (Cl) 0.81–2.55] and woodworking (OR 1.70 with 95% Cl 0.67–4.31) but not with farming as the father's occupation (OR 0.96 with 95% Cl 0.66–1.41). It was also more frequent in migrants to Australia than in native‐born Australians (OR 1.31 with 95% Cl 0.99–1.74) particularly those born in England (OR 1.57 with 95% Cl 1.11–2.42) and Italy (OR 2.02 with 95% Cl 1.09–3.74). An apparent excess of multiple myeloma in all rural residents appeared to be due to bias introduced by matching for death registration district, as rural residents dying from multiple myeloma were more likely to die and have their deaths registered in the city than other rural residents. A conditional logistic regression analysis showed the above associations to be substantially independent of one another.
Comparison of doxorubicin‐induced DNA damage in doxorubicin‐sensitive and ‐resistant P388 murine leukemia cells
Authors: Capranico Giovanni , Dasdia Teresa , Zunino Franco 
Published: February 2007

AbstractDoxorubicin‐induced DNA damage was studied in the P388 leukemia cell line and in a doxorubicin‐resistant subline by alkaline elution techniques. DNA single‐strand breaks and DNA‐protein cross‐links were determined. Whereas, in the sensitive line, I hr exposure to drug induced DNA damage in a concentration‐dependent manner, in the resistant line only a marginal effect was observed at high drug concentrations. In contrast, elution kinetics of DNAs from cells irradiated with X‐rays were similar in both lines. Although a reduced intracellular drug accumulation was found in resistant cells, this difference could not account for the marked reduction in doxorubicin‐induced DNA damage. The degree of resistance of the P388 subline was reduced about 7‐fold by verapamil, whereas the extent of DNA damage was unaffected. These results suggest the presence of alternative modes of resistance, independent of membrane changes, in highly resistant cells.
Analysis of structural polypeptides of the lymphoproliferative disease virus (LPDV) of turkeys
Authors: Gazit Arnona , Basri Ruth , Ianconescu Marius , Perk Kalman , Zimber Amatzia , Yaniv Abraham 
Published: February 2007

AbstractThe polypeptide composition of the lymphoproliferative disease virus (LPDV) of turkeys was shown to comprise several polypeptides with apparent molecular weights of 76, 31, 28, 20 and 15 kDa. This polypeptide pattern is distinctly different from the protein profiles of avian leukosis viruses, reticuloendotheliosis virus, or murine leukemia viruses. Moreover, LPD virions contain 2 major structural proteins (p31 and p28), in contrast to only one major internal protein present in most other retroviruses. The 76 kDa protein was established as the major viral envelope glycoprotein. The uniqueness of the LPDV polypeptide pattern is consistent with the lack of genetic relatedness of LPDV genome to other retroviruses, establishing LPDV as a representative of a distinct group of retroviridae.
Elimination of murine erythroleukemic stem cells with a novel anti‐erythroid antibody conjugated to ricin a‐chain: A model for studies of bone‐marrow transplantation therapy
Authors: Tonevitsky A. G. , Mechetner E. B. , Rozinova Emilia N. , Ievleva Elena S. , Poltoranina Valentina S. 
Published: February 2007

AbstractWe have produced a rat monoclonal antibody (MAb) MAE 15 (IgG), specific for murine erythroid cells, using a murine erythroid cell line as immunogen. This MAb specifically binds to the surface of normal and neoplastic murine erythroid cells. Murine mature erythrocytes and non‐erythroid cells as well as rat and human erythroid and non‐erythroid cells are not recognized by MAb MAE 15. Immunoblotting analysis and mixed precipitation in agar gel showed MAb MAE 15 to be specific for murine epitope of 69 kDa antigen of erythroblasts (Ag‐Eb), an interspecies antigenic marker of nucleated red cells and reticulocytes. A conjugate (immunotoxin) was prepared, comprising ricin A‐chain and MAb MAE15. The immunotoxin inhibited protein synthesis of murine erythroleukemic Ag‐Eb‐positive K‐2 cells and completely inhibited (at the concentration of 2 × 10−7 m) spleen colony formation by erythroleukemic stem cells of the Ag‐Eb‐positive RAL cell line. Approximately 35% of the murine normal stem‐cell (CFU‐S) population was not affected by the immunotoxin at the concentration of 2 × 10−7 m. This experimental system may be a convenient model for studies of bone marrow transplantation therapy of erythroleukemias.
In situ identification of host derived infiltrating cells in chemically induced fibrosarcomas of interspecific chimeric mice
Authors: Deamant F. D. , Vijh M. , Rossant J. , Iannaccone P. M. 
Published: February 2007

AbstractChimeric mice have been used to investigate the cellular origin of chemically induced tumors. Interspecific chimeras were formed between the Ha/ICR strain of Mus musculus and Mus caroli. The normal tissues of the chimeric mice were composed of a mixture of cells which originated from each of the 2 species. The species of origin of the cells was determined both histologically with in situ hybridization using a DNA probe which recognizes M. musculus satellite DNA and by electrophoretic analysis of phosphoglycerate kinase‐I (PGK‐I) isozyme expression. The 2 parental species, M. musculus and M. caroli, had comparable levels of inducible cytochrome P‐450‐dependent mixed function oxidase activity. Subcutaneous fibrosarcomas were induced with 3‐methylcholanthrene. Electrophoretic analysis of many samples from the tumors revealed that some demonstrated both isozymes of PGK‐I. However, extensive sectioning with subsequent in situ hybridization revealed that the only cells of mixed genotype were small, infiltrating inflammatory cells. These results confirmed PGK‐I analysis of the tissue culture cell lines derived from the tumors. This is the first report of direct visualization of the lineage origin of various cellular components in chemically induced tumors in a mosaic system and supports the contention that, while the neoplastic cells are clonal in origin, the tumors also contain non‐neoplastic “host” cells which are derived from both sets of parents.
Differential antiproliferatve effects of combinations of recombinant interferons alpha and gamma on two murine tumor cell lines
Authors: Brunda Michael J. , Wright Rosemary B. 
Published: February 2007

AbstractThe in vitro antiproliferative effects of recombinant interferons (IFNs) alpha and gamma against the murine reticulum‐cell sarcoma M5076 and the malignant melanoma B16 F10 were evaluated using the human hybrid IFN‐alpha A/D (rHulFN‐αA/D), which is active on murine cells, and recombinant murine IFN gamma (rMulFN‐γ). An isobologram analysis was used to evaluate the interactive antiproliferative effects of the recombinant IFNs on these two tumor cell lines. The data, in contrast to prior reports, indicate that rHulFN‐αA/D and rMulFN‐γ interact in an additive rather than a synergistic manner against M5076 cells. When a similar analysis was performed on B16 F10 cells, synergy was obtained. Thus, either a synergistic or an additive antiproliferative effect can be obtained by combining IFN‐α and IFN‐γ, depending upon the cell line used in the assay.
Cloning and characterization of putative genes that specify sensitivity to neoplastic transformation by tumor promoters
Authors: Lerman Michael I. , Hegamyer Glenn A. , Colburn Nancy H. 
Published: February 2007

AbstractTwo putative genes (termed pro 1 and pro 2) specifying sensitivity to induction of neoplastic transformation by TPA in mouse epidermal JB6 cells were cloned by sib selection from a size‐selected genomic library of clonal cells sensitive to promotion of transformation. By restriction analysis, heteroduplex analysis, direct hybridization, and sequencing, the putative genes are different from and have no homology to known oncogenes. Both genes are independently and equally active as total DNA in the transfection assay. The transformation‐promoting potential of these putative genes does not appear to result from gene amplification or detectable rearrangements, suggesting that small structural changes might confer the promoting activity. The mouse pro sequences are also found in monkey and human DNAs. The pro‐1 sequence is homologous to middle repetitive elements in the mouse genome, namely the BAM 5 and B1 repeats. The sequence of pro‐1 was determined and suggests that it contains the signals to be transcribed by RNA polymerase 11 and to encode a protein of 7.1 kDa.
Correlation between quantitative expression of H‐2K, H‐2D and mulv antigens on spontaneous AKR lymphomas
Authors: Oudshoorn‐Snoek Margriet , Demant Peter 
Published: February 2007

AbstractThymocytes of leukemic AKR mice were analysed with a fluorescence‐activated cell sorter (FACS) using monoclonal antibodies (MAbs) reactive with H‐2Kk and H‐2Dk and conventional hetero‐antibodies against MuLV gp‐70 antigens. Comparison was made with thymocytes of the low‐leukemia H‐2k strain C3H, as well as with thymocytes of young AKR mice, late preleukemic AKR mice (6–9 months old) and mitogen‐stimulated thymocytes of young AKR mice. Expression of H‐2 antigens increased on cells of the majority of tumors, on thymocytes of some late preleukemic mice and on mitogen‐stimulated thymocytes. An increase in H‐2K and H‐2D antigens was noted: imbalance of the K/D ratio in favor of H‐2D was observed mostly in tumors with relatively lower total amounts of H‐2K and D antigens; ratio shifts in favor of H‐2K were also found, mostly in tumors with relatively higher amounts of H‐2K and D antigens. Increased expression of MuLV antigens was found on cells of all tumors and on thymocytes of several late preleukemic mice. We show that in primary spontaneous AKR leukemias, in spite of large individual differences, the expression of high amounts of MuLV gp70 is not random, but is associated with high expression of H‐2K and H‐2D antigens. The same phenomenon is seen in thymocytes of preleukemic mice, but in mitogen‐stimulated thymus cells increase of H‐2 expression is not accompanied by increase of MuLV gp70.
Suppression of human lymphocyte mitogen response by proteins of the type‐D retrovirus PMFV
Authors: Denner Joachim , Wunderlich Volker , Bierwolf Dieter 
Published: February 2007

AbstractA type‐D retrovirus, derived from a human cell line and designated PMFV, which is related to, but distinct from the Mason‐Pfizer monkey virus (MPMV) suppressed the in vitro mitogen response of human lymphocytes. PMFV was suppressive either as intact virus or after disruption by ether or detergents. In kinetic studies, the time course of the interaction between suppressive virus components and lymphocytes was characterized. Neither cytotoxicity nor time shifts in optimum 3H‐thymidine incorporation of responding lymphocytes were observed. The suppressive activity of the disrupted virus was diminished by heating, freeze‐thawing or treatment with pronase or trypsin, indicating temperature‐sensitive proteins as suppressive components. By Sephacryl S‐200 gel chromatography, molecular weights of approximately 70 and 15 kDa were determined for the suppressive components which were isolated from disrupted virus or virus‐producing cells but not from non‐infected cells.
Clinical analysis of 33 patients with adult T‐cell leukemia (ATL)‐diagnostic criteria and significance of high‐ and low‐risk ATL
Authors: Tamura Kazuo , Nagamine Nobuhiko , Araki Yasuhiko , Seita Masashi , Okayama Akihiko , Kawano Kiyohide , Tachibana Nobuyoshi , Tsuda Kazunori , Kuroki Yasumasa , Narita Hiromi , Inoue Shouhei , Suzumiya Junji , Sumiyoshi Akinobu , Aratake Yatsuki , Ohtaki Sachiya , Torigoe Junichiro , Kawachi Jitsuyo 
Published: February 2007

AbstractThe clinical characteristics of 33 patients with adult T‐cell leukemia (ATL) are described. All patients were born and have lived in Miyazaki Prefecture (south‐west of Japan). Because of a wide range of clinical presentations and courses, they were subdivided into 2 groups. In the high‐risk group, patients presented with high white‐cell counts (WBC ≧ 20,000/μl) and over 30% of abnormal lymphoid cells (18 patients) and hypercalcemia with a low percentage of leukemic cells (5 patients). In this group the median survival time was only 3 months despite various modes of treatment. In contrast, patients of the second group exhibited a low percentage of abnormal lymphoid cells (WBC < 20,000/μl and/or leukemic cells < 30%) and had no hypercalcemia (8 patients). Their clinical course was chronic with a median survival of 8 months, regardless of modalities of treatment. Two patients went through a period when the number of circulating leukemic cells was low ( ≦ 5%) before overt leukemia appeared. Other clinical features, signs, symptoms, routine laboratory data, serum anti‐ATL‐associated antibody, cell membrane markers and cytogenetic studies were similar to those observed in other districts of Kyushu island.