bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agonists of Toll-Like Receptors 3, 4, 7, and 9 Are Candidates for Use as Adjuvants in an Outer Membrane Vaccine againstNeisseria meningitidisSerogroup B
Authors: Fransen Floris , Boog Claire J. , van Putten Jos P. , van der Ley Peter 
Published: October 2007

ABSTRACTThe bacteriumNeisseria meningitidisis the causative agent of meningitis and sepsis. A generally effective vaccine againstN. meningitidisserogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion ofN. meningitidiswild-type LPS in a vaccine is controversial because of its high toxicity. Therefore, the adjuvant activity of a panel of different Toll-like receptor (TLR) agonists in combination with LPS-deficient meningococcal outer membrane complexes was compared after immunization of mice. The results demonstrate that TLR3, TLR4, TLR7, and TLR9 agonists enhance immune responses against LPS-deficient outer membrane complexes. Their adjuvant activity was characterized by higher levels of antigen-specific immunoglobulin G (IgG), IgG2a, and IgG2b; a higher IgG2a/IgG1 ratio; lower total IgE levels; and most importantly, higher serum bactericidal antibody titers compared to LPS-deficient outer membrane complexes alone.
Chlamydia pneumoniae
Inclusion Membrane Protein Cpn0585 Interacts with Multiple Rab GTPases
Authors: Cortes Claudio , Rzomp Kimberly A. , Tvinnereim Amy , Scidmore Marci A. , Wizel Benjamin 
Published: October 2007

ABSTRACT

Chlamydiae are intracellular bacteria that develop within a membrane-bound vacuole called an inclusion. To ensure that the inclusion is a safe niche for chlamydial replication, chlamydiae exploit a number of host cell processes, including membrane-trafficking pathways. Recently, several Rab GTPases were found to associate with the inclusions of various chlamydial species. Here we report that Cpn0585, a
Chlamydia pneumoniae
inclusion membrane protein (Inc), interacts with multiple Rab GTPases. The results from yeast two-hybrid experiments revealed that an amino-terminally truncated form of Cpn0585 (Cpn0585
102-651
) interacts with Rab1, Rab10, and Rab11 but not with Rab4 or Rab6. Cpn0585-Rab GTPase interactions are direct and GTP dependent as shown in glutathione
S
-transferase pull-down assays using native and recombinant Cpn0585. In
C. pneumoniae
-infected HEp-2 cells transfected with enhanced green fluorescent protein (EGFP)-tagged Rab GTPases, the colocalization with Cpn0585 at the inclusion membrane was partial for EGFP-Rab1 and EGFP-Rab10, but extensive for wild-type EGFP-Rab11A and the constitutively active GTPase-deficient EGFP-Rab11AQ70L. Moreover, Cpn0585 colocalized with EGFP-Rab11AQ70L as early as 2 h postinfection. Upon delivery into live
C. pneumoniae
-infected cells, Cpn0585
628-651
-specific antibodies bound to the inclusion membrane, demonstrating that the Rab GTPase-interacting domain of Cpn0585 faces the host cell cytosol. Finally, ectopic expression of Cpn0585
102-651
partially inhibited the development of
C. pneumoniae
inclusions in EGFP. but not in EGFP-Rab11AQ70L-expressing HEp-2 cells. Collectively, these data suggest that Cpn0585 is involved in the recruitment of Rab GTPases to the inclusion membrane and that interfering with this function may adversely impact the fitness of the
C. pneumoniae
inclusion for chlamydial replication.
Chlamydia trachomatis
Species-Specific Induction of Ezrin Tyrosine Phosphorylation Functions in Pathogen Entry
Authors: Swanson Kena A. , Crane Deborah D. , Caldwell Harlan D. 
Published: October 2007

ABSTRACT

Chlamydia trachomatis
is an obligate intracellular pathogen of humans that exhibits species-specific biological characteristics in its early interactions with host cells that are likely important to pathogenesis. One such characteristic is the tyrosine phosphorylation (Tyr-P) of an ∼70-kDa polypeptide that occurs only after infection of mammalian cells by human strains. We sought to identify this protein because of its potential significance to the pathogenesis of human chlamydial infections. Using an immunoproteomic approach we identified the host protein ezrin, a member of the ezrin-radixin-moesin (ERM) protein family that serves as a physical link between host cell receptors and the actin cytoskeleton. Confocal microscopy studies showed colocalization of ezrin and actin at the tips and crypts of microvilli, the site of chlamydial attachment and entry, respectively. To demonstrate a functional role for ezrin we infected cells with a dominant-negative (DN) ezrin phenotype or treated cells with ezrin-specific small interfering RNA (siRNA). We found that both DN and siRNA-treated cells were significantly less susceptible to infection by human chlamydial strains. Moreover, we demonstrated that inhibition of infection in ezrin DN cells occurred at the stage of chlamydial entry. We hypothesize that the
C. trachomatis
-specific Tyr-P of ezrin might relate to an undefined species-specific mechanism of pathogen entry that involves chlamydial specific ligand(s) and host cell coreceptor usage.
A Study of Regional Precipitation Trends in Iceland Using a High-Quality Gauge Network and ERA-40
Authors: Crochet Philippe 
Published: October 2007

Abstract
The climatic variability and trends of precipitation in Iceland are examined for the period 1961–2000 using bias-corrected rain gauge data and precipitation forecasts from the 40-yr ECMWF Re-Analysis (ERA-40). Several quantities are studied: the monthly and annual precipitation amounts as well as the statistical characteristics of 12-hourly precipitation rate and the geometrical features of the precipitation fields. The study highlights the credibility of ERA-40 and its ability to capture in a realistic manner the climatic variability of precipitation in Iceland, despite local discrepancies related to the crude horizontal resolution of these data. The observed intra- and interannual variations in the precipitation characteristics can be linked to variations in size, location, and occurrence of the large-scale precipitation systems crossing Iceland that are in turn influenced by the strength of the North Atlantic Oscillation, especially in winter.
The Central American Midsummer Drought: Regional Aspects and Large-Scale Forcing*
Authors: Small Richard Justin O. , de Szoeke Simon P. , Xie Shang-Ping 
Published: October 2007

Abstract
The midsummer drought (MSD) is a diminution in rainfall experienced during the middle of the rainy season in southern Mexico and Central America, as well as in the adjacent Caribbean, Gulf of Mexico, and eastern Pacific seas. The aim of this paper is to describe the regional characteristics of the MSD and to propose some possible forcing mechanisms. Satellite and in situ data are used to form a composite of the evolution of a typical MSD, which highlights its coincidence with a low-level anticyclone centered over the Gulf of Mexico and associated easterly flow across Central America. The diurnal cycle of precipitation over the region is reduced in amplitude during midsummer. The MSD is also coincident with heavy precipitation over the Sierra Madre Occidental (part of the North American monsoon). Reanalysis data are used to show that the divergence of the anomalous low-level flow during the MSD is the main factor governing the variations in precipitation. A linear baroclinic model is used to show that the seasonal progression of the Pacific intertropical convergence zone (ITCZ), which moves northward following warm sea surface temperature (SST) during the early summer, and of the Atlantic subtropical high, which moves westward, are the most important remote factors that contribute toward the low-level easterly flow and divergence during the MSD. The circulation associated with the MSD precipitation deficit helps to maintain the deficit by reinforcing the low-level anticyclonic flow over the Gulf of Mexico. Surface heating over land also plays a role: a large thermal low over the northern United States in early summer is accompanied by enhanced subsidence over the North Atlantic. This thermal low is seen to decrease considerably in midsummer, allowing the high pressure anomalies in the Atlantic and Pacific Oceans to extend into the Gulf of Mexico. These anomalies are maintained until late summer, when an increase in rainfall from the surge in Atlantic tropical depressions induces anomalous surface cyclonic flow with westerlies fluxing moisture from the Pacific ITCZ toward Central America.
How Often Will It Rain?
Authors: Sun Ying , Solomon Susan , Dai Aiguo , Portmann Robert W. 
Published: October 2007

Abstract
Daily precipitation data from climate change simulations using the latest generation of coupled climate system models are analyzed for potential future changes in precipitation characteristics. For the Intergovernmental Panel on Climate Change (IPCC) Special Report on Emissions Scenarios (SRES) B1 (a low projection), A1B (a medium projection), and A2 (a high projection) during the twenty-first century, all the models consistently show a shift toward more intense and extreme precipitation for the globe as a whole and over various regions. For both SRES B1 and A2, most models show decreased daily precipitation frequency and all the models show increased daily precipitation intensity. The multimodel averaged percentage increase in the precipitation intensity (2.0% K−1) is larger than the magnitude of the precipitation frequency decrease (−0.7% K−1). However, the shift in precipitation frequency distribution toward extremes results in large increases in very heavy precipitation events (&gt;50 mm day−1), so that for very heavy precipitation, the percentage increase in frequency is much larger than the increase in intensity (31.2% versus 2.4%). The climate model projected increases in daily precipitation intensity are, however, smaller than that based on simple thermodynamics (∼7% K−1). Multimodel ensemble means show that precipitation amount increases during the twenty-first century over high latitudes, as well as over currently wet regions in low- and midlatitudes more than other regions. This increase mostly results from a combination of increased frequency and intensity. Over the dry regions in the subtropics, the precipitation amount generally declines because of decreases in both frequency and intensity. This indicates that wet regions may get wetter and dry regions may become drier mostly because of a simultaneous increase (decrease) of precipitation frequency and intensity.
Southeast Asian Pressure Surges and Significant Events of Atmospheric Mass Loss from the Northern Hemisphere, and a Case Study Analysis
Authors: Carrera Marco L. , Gyakum John R. 
Published: October 2007

Abstract
A recent study of significant events of atmospheric mass depletion from the Northern Hemisphere (NH) during the extended boreal winter indicated that Southeast Asian pressure surges were an important physical mechanism that acted to channel the atmospheric mass equatorward out of the NH on a rapid time scale. This study builds upon this finding and examines both the direct and indirect roles of Southeast Asian pressure surges for a particular event of dry atmospheric mass depletion from the NH. The focus of this study is on the enhanced interhemispheric interactions and associated Southern Hemisphere (SH) tropical and extratropical responses resulting from the pressure surges.
First, this study examines the conservation of dry atmospheric mass (i.e., the relationship between the dry meridional winds and the area-integrated dry air surface pressure) in the NCEP reanalysis for the 25 significant events of dry atmospheric mass depletion from the NH. Results indicate that the NCEP dry meridional winds are able to qualitatively capture the dry atmospheric mass evacuation from the NH. In a quantitative sense there is very good agreement between the wind and pressure data in the extratropics of both hemispheres. A distinct negative or southward bias in the NCEP vertically and zonally integrated dry meridional winds is apparent between 5° and 17.5°N. This southward bias was not present in the ECMWF Re-Analysis. The source of the southward bias in NCEP appears to result from a weaker analyzed ITCZ.
The particular case of dry atmospheric mass depletion from the NH examined in detail is associated with an intense pressure surge over Southeast Asia. A significant enhancement of convection in the monsoon trough region of northern Australia occurs roughly 4 days after the peak intensity of the Siberian high. A low-level westerly wind burst develops in response to this enhanced zonal pressure gradient caused by the pressure surge as part of the onset of an active phase of the Australian summer monsoon. This study shows that three prominent anticyclonic circulations intensify in the SH extratropics, stretching from the south Indian Ocean to the South Pacific, beneath regions of upper-tropospheric dry atmospheric mass convergence, originating partly from the monsoon convection outflow. These anticyclonic circulations are regional manifestations of the dry atmospheric mass increase in the SH.
Influence of the Arctic Circumpolar Vortex on the Mass Balance of Canadian High Arctic Glaciers
Authors: Gardner Alex S. , Sharp Martin 
Published: October 2007

AbstractVariability in July mean surface air temperatures from 1963 to 2003 accounted for 62% of the variance in the regional annual glacier mass balance signal for the Canadian High Arctic. A regime shift to more negative regional glacier mass balance occurred between 1986 and 1987, and is linked to a coincident shift from lower to higher mean July air temperatures. Both the interannual changes and the regime shifts in regional glacier mass balance and July air temperatures are related to variations in the position and strength of the July circumpolar vortex. In years when the July vortex is “strong” and its center is located in the Western Hemisphere, positive mass balance anomalies prevail. In contrast, highly negative mass balance anomalies occur when the July circumpolar vortex is either weak or strong without elongation over the Canadian High Arctic, and its center is located in the Eastern Hemisphere. The occurrence of westerly positioned July vortices has decreased by 40% since 1987. The associated shift to a dominantly easterly positioned July vortex was associated with an increased frequency of tropospheric ridging over the Canadian High Arctic, higher surface air temperatures, and more negative regional glacier mass balance.
Seasonally Stratified Analysis of Simulated ENSO Thermodynamics
Authors: Tozuka Tomoki , Luo Jing-Jia , Masson Sebastien , Yamagata Toshio 
Published: October 2007

Abstract
Using outputs from the SINTEX-F1 coupled GCM, the thermodynamics of ENSO events and its relation with the seasonal cycle are investigated. Simulated El Niño events are first classified into four groups depending on during which season the Niño-3.4 sea surface temperature anomaly (SSTA) index (5°S–5°N, 120°–170°W) reaches its peak. Although the heat content of the tropical Pacific decreases for all four types, the tropical Pacific loses about twice as much during an El Niño that peaks during winter compared with one that peaks during summer. The surface heat flux, the southward heat transport at 15°S, and the Indonesian Throughflow heat transport contribute constructively to this remarkable seasonal difference. It is shown that the Indonesian Throughflow supplies anomalous heat from the Indian Ocean, especially during the summer El Niño–like event. Changes in the basic state provided by the seasonal cycle cause differences in the atmospheric response to the SSTA, which in turn lead to the difference between the surface heat flux and the meridional heat transport anomaly.
A Predictability Measure Applied to Seasonal Predictions of the Arctic Oscillation
Authors: Tang Youmin , Lin Hai , Derome Jacques , Tippett Michael K. 
Published: October 2007

AbstractIn this study, ensemble seasonal predictions of the Arctic Oscillation (AO) were conducted for 51 winters (1948–98) using a simple global atmospheric general circulation model. A means of estimating a priori the predictive skill of the AO ensemble predictions was developed based on the relative entropy (R) of information theory, which is a measure of the difference between the forecast and climatology probability density functions (PDFs). Several important issues related to the AO predictability, such as the dominant precursors of forecast skill and the degree of confidence that can be placed in an individual forecast, were addressed. It was found that R is a useful measure of the confidence that can be placed on dynamical predictions of the AO. When R is large, the prediction is likely to have a high confidence level whereas when R is small, the prediction skill is more variable. A small R is often accompanied by a relatively weak AO index. The value of R is dominated by the predicted ensemble mean. The relationship identified here, between model skills and the R of an ensemble prediction, offers a practical means of estimating the confidence level of a seasonal forecast of the AO using the dynamical model.Through an analysis of the global sea surface temperature (SST) forcing, it was found that the winter AO-related R is correlated significantly with the amplitude of the SST anomalies over the tropical central Pacific and the North Pacific during the previous October. A large value of R is usually associated with strong SST anomalies in the two regions, whereas a poor prediction with a small R indicates that SST anomalies are likely weak in these two regions and the observed AO anomaly in the specific winter is likely caused by atmospheric internal dynamics.
Human Impact on Direct and Diffuse Solar Radiation during the Industrial Era
Authors: Kvalevåg Maria M. , Myhre Gunnar 
Published: October 2007

AbstractIn this study the direct and diffuse solar radiation changes are estimated, and they contribute to the understanding of the observed global dimming and the more recent global brightening during the industrial era. Using a multistream radiative transfer model, the authors calculate the impact of changes in ozone, NO2, water vapor, CH4, CO2, direct and indirect aerosol effects, contrails, and aviation-induced cirrus on solar irradiances at the surface. The results show that dimming is most pronounced in central Africa, Southeast Asia, Europe, and northeast America. Human activity during the industrial era is calculated and accounts for a decrease in direct solar radiation at the surface of up to 30 W m−2 (30%–40%) and an increase in diffuse solar radiation of up to 20 W m−2. The physical processes that lead to the changes in direct and diffuse solar radiation are found to be remarkably different and the authors explain which mechanisms are responsible for the observed changes.
Enhancement of ENSO Variability by a Weakened Atlantic Thermohaline Circulation in a Coupled GCM
Authors: Dong Buwen , Sutton Rowan T. 
Published: October 2007

Abstract
A coupled ocean–atmosphere general circulation model is used to investigate the modulation of El Niño–Southern Oscillation (ENSO) variability due to a weakened Atlantic thermohaline circulation (THC). The THC weakening is induced by freshwater perturbations in the North Atlantic, and leads to a well-known sea surface temperature dipole and a southward shift of the intertropical convergence zone (ITCZ) in the tropical Atlantic. Through atmospheric teleconnections and local coupled air–sea feedbacks, a meridionally asymmetric mean state change is generated in the eastern equatorial Pacific, corresponding to a weakened annual cycle, and westerly anomalies develop over the central Pacific. The westerly anomalies are associated with anomalous warming of SST, causing an eastward extension of the west Pacific warm pool particularly in August–February, and enhanced precipitation. These and other changes in the mean state lead in turn to an eastward shift of the zonal wind anomalies associated with El Niño events, and a significant increase in ENSO variability.
In response to a 1-Sv (1 Sv ≡ 106 m3 s−1) freshwater input in the North Atlantic, the THC slows down rapidly and it weakens by 86% over years 50–100. The Niño-3 index standard deviation increases by 36% during the first 100-yr simulation relative to the control simulation. Further analysis indicates that the weakened THC not only leads to a stronger ENSO variability, but also leads to a stronger asymmetry between El Niño and La Niña events. This study suggests a role for an atmospheric bridge that rapidly conveys the influence of the Atlantic Ocean to the tropical Pacific and indicates that fluctuations of the THC can mediate not only mean climate globally but also modulate interannual variability. The results may contribute to understanding both the multidecadal variability of ENSO activity during the twentieth century and longer time-scale variability of ENSO, as suggested by some paleoclimate records.
Tests of Monte Carlo Independent Column Approximation in the ECHAM5 Atmospheric GCM
Authors: Räisänen P. , Järvenoja S. , Järvinen H. , Giorgetta M. , Roeckner E. , Jylhä K. , Ruosteenoja K. 
Published: October 2007

Abstract
The Monte Carlo Independent Column Approximation (McICA) method for computing domain-average radiative fluxes allows a flexible treatment of unresolved cloud structure, and it is unbiased with respect to the full ICA, but its flux estimates contain conditional random noise. Here, tests of McICA in the ECHAM5 atmospheric GCM are reported. ECHAM5 provides an interesting test bed for McICA because it carries prognostic variables for the subgrid-scale probability distribution of total water content, which allows us to determine subgrid-scale cloud variability directly from the resolved-scale model variables.
Three experiments with differing levels of radiative noise, each consisting of ten 6-yr runs, are performed to estimate the impact of McICA noise on simulated climate. In an experiment that attempted to deliberately maximize McICA noise, a systematic reduction in low cloud fraction occurred. For a more reasonable implementation of McICA, the impact of noise is very small, although statistically discernible.
In terms of the impacts of noise, McICA appears to be a viable approach for use in ECHAM5. However, to improve the simulation of cloud radiative effects, realistic representation of both unresolved and resolved cloud structures is needed, which remains a challenging problem. Comparison of ECHAM5 data with a global cloud system–resolving model dataset and with International Satellite Cloud Climatology Project data suggested two problems related to unresolved cloud structures. First, ECHAM5 appears to underestimate subgrid-scale cloud variability. This problem seems partly related to the use of the beta distribution scheme for total water content in ECHAM5: in its current form, the scheme is unable to generate highly inhomogeneous clouds (relative standard deviation of condensate amount &gt;1). Second, it appears that in ECHAM5, overcast cloud layers occur too frequently and partially cloudy layers too rarely. This problem is not unique to the beta distribution scheme; in fact, it is more pronounced when using an alternative, relative humidity–based cloud fraction scheme.