bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract LB430: Identification of therapeutic target in breast cancer using G-quadruplex binding ligands
Authors: Razzaq Maria , Ravichandran Subramaniyam , Parveen Nazia , Kim Kyeong Kyu 
Published: April 2024

Abstract
G-quadruplex (G4) motifs are noncanonical nucleic acid structures formed from staked guanine rich tetrads. G4 motifs have been shown to play significant effects on gene expression in all domains of life and are predominantly enriched in the regulatory regions such as promoters and enhancers of the human genome. G4-ligand mediated stabilization or destabilization of G4 structures has been discussed as a potential therapeutic strategy against tumor cells. However, the structure-specificity of G4-binding ligands remains a considerable challenge in determining the target-specificity. Therefore, it is necessary to understand the global effects of such G4-binding ligands at the transcriptome level to identify novel target(s) based on molecular structures and binding modes of both G4 motifs and ligands. In this study, we reported a top-down approach to extrapolate transcriptome-wide effects of two eminent G4-binding ligands Quarfloxin and Pyridostatin in human breast adenocarcinoma (MCF7) cell lines by RNA-seq analysis. Quadparser analysis was performed to analyze putative G4 forming sequences in protein coding breast cancer genes promoters. RNA-seq data was combined with Quadparser analysis to reveal that over 85% of the differentially expressed genes in both Pyridostatin and Quarfloxin contained G4 forming sequences in promoter region. Gene ontology analysis showed that both ligands affected key genes involved in extracellular matrix (ECM), architecture and cellular functions. A notable tumor suppressor deubiquitinase (DUBs) Cylindromatosis (CYLD) gene expression was particularly increased with Pyridostatin compared to Quarfloxin. In vitro assays also confirmed an increase in CYLD expression by qRT-PCR and Western blot. The detailed molecular mechanism of G4-mediated increase in CYLD expression is under study. Collectively, the current results present a new approach to identify G4-ligands binding target in breast cancer cells and provide insights into designing G4-ligands as an anti-cancer therapeutics.
Citation Format: Maria Razzaq, Subramaniyam Ravichandran, Nazia Parveen, Kyeong Kyu Kim. Identification of therapeutic target in breast cancer using G-quadruplex binding ligands [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LB430.
Abstract LE21: Mechanisms of gene regulation by chromatin adaptors in development and disease
Authors: Soto-Feliciano Yadira Marie 
Published: April 2024

Abstract
Complex biological processes, such as organismal development, are directly influenced by the interplay between genes and the cellular structures and environmental factors that regulate them. Key players in this regulation are chromatin adaptor proteins, which can impact the genetic and epigenetic programs of cells. However, our understanding of how these proteins interact with chromatin - the physiological form of our genomes - and regulate gene activity is limited, especially in the context of diseases like cancer.
To test our central hypothesis and to understand how chromatin adaptors contribute to cell function, we are developing innovative high-throughput and biochemical approaches. These will help us systematically identify specific histone modifications that affect the binding of Menin and other adaptors to chromatin. Our experimental approach includes profiling, validation, and functional characterization using advanced genomic and epigenomic technologies, transcriptional profiling, and CRISPR gene editing. The application of these techniques will reveal, for the first time, the molecular mechanisms by which these proteins bind to specific parts of the genome and regulate gene activity.
Our findings will enhance our understanding of how chromatin adaptors, like Menin, interact with chromatin and regulate gene activity in different tissues, and how these functions are disrupted by cancer-associated mutations. This will also contribute to the development of potential new therapies that target these altered mechanisms. Furthermore, our findings can be extended to other proteins involved in chromatin and epigenetic regulation, enabling a broader application in understanding and treating cancer and other human diseases.
Citation Format: Yadira Marie Soto-Feliciano. Mechanisms of gene regulation by chromatin adaptors in development and disease [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr LE21.
Abstract SY31-02: Nociceptor neurons control anti-tumor immunity
Authors: Eichwald Tuany , Balood Mohammad , Ahmadi Maryam , Roversi Karine , Wong Dylan , Mandl Judith , Rafei Moutih , Ghasemlou Nader , Vermeer Paola , Amit Moran , Talbot Sébastien 
Published: April 2024

Abstract
Cancer is the top cause of death in the USA, responsible for 30% of deaths. A cure remains undiscovered. Therapy effectiveness varies by patient and tumor type and can have negative side effects. The immune system, designed to eliminate abnormal cells, including cancer cells, is often impaired. For example, in skin cancer, extended activation of certain receptors can exhaust immune cells, reducing their cancer-fighting ability. Our lab found that cancer cells trigger nociceptor neurons in tumors, leading to neuropeptide release that exhausts CD8+ T cells. To explore their interactions, we conducted single-cell RNA sequencing on tumor-related sensory neurons, leukocytes, and cancer cells. We found a distinct group of tumor-related neurons expressingATF3, Sox10, Sprr1a, and galanin. In our study, mice with injury-resistant nociceptor neurons (NaV1.8ΔATF3) developed smaller melanoma tumors and had reduced galanin levels compared to controls. These mice also showed stronger anti-tumor immune responses, especially in cytotoxic CD8+ T-cells. Restoring galanin in mice with ATF3-deficient nociceptor neurons resulted in tumor growth similar to normal mice. We observed that galanin increases exhaustion, reduces the cytotoxic capacity of CD8+ T-cells, and leads the cells to a state of immunoparalysis. Higher galanin levels in melanoma patients were linked to worse outcomes. Using single-cell RNA sequencing from melanoma patients, we also found that cytotoxic CD8+ T cells with the galanin receptor 1(GALR1) were more exhausted than those without it. In summary, ATF3-modifiedtumor-related sensory neurons that release galanin can weaken anti-tumor immunity, suggesting cancer cells may use this aspect of neuro-immunity to promote tumor growth.
Citation Format: Tuany Eichwald, Mohammad Balood, Maryam Ahmadi, Karine Roversi, Dylan Wong, Judith Mandl, Moutih Rafei, Nader Ghasemlou, Paola Vermeer, Moran Amit, Sébastien Talbot. Nociceptor neurons control anti-tumor immunity [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr SY31-02.
Abstract SY34-02: Age-related and organ-specific host mosaicism as a driver of dormant cancer cell awakening
Authors: Aguirre-Ghiso Julio A. , Papanicolaou Michael , Vela Pablo Sánchez , Yang Julie , Safonov Anton , Tao Jacqueline , Exposito Sebastia Franch , Tiedje Vera , Dalla Erica , Salazar Luis E. Valencia , Barth Nicole , Sahin Merve , Chen Kevin , Park Jinae , Wereski Matthew , Krishnan Aishwarya , Benitez Anthony Martinez , Ptashkin Ryan , Robson Mark E. , de Stanchina Elisa , Braunstein Lior Z. , Zehir Ahmet , Comen Elizabeth , Norton Larry , Berger Michael , Koche Richard , Razavi Pedram , Levine Ross 
Published: April 2024

Abstract
Most BC patients succumb to metastatic relapse years to decades following primary tumor resection due to reactivation of dormant disseminated cancer cells (DCCs). Improved BC patient survival has led to metastatic relapse manifesting in an older patient population. This population carries somatic mosaicism in many organs including bone marrow. Age-specific somatic mutations (e.g., DNMT3A, ASXL1, TET2) in hematopoietic stem and progenitor cells (HSPCs) results in CH, which is associated with increased inflammatory myeloid cells and risk of leukemia and intriguingly with BC-specific mortality. In this presentation I will discuss new data from a team effort to test the hypothesis that CH myeloid cells could shape target organs niches that harbor dormant DCCs to be pro-metastatic. This hypothesis has been explored in clinical trials data where the presence of CH in ER+ and HER2+ metastatic BC patient cohorts (MSK-IMPACT) was associated with the response to first line CDK4/6i/endocrine therapy or taxol/herceptin/pertuzumab, respectively. We also tested whether CH can accelerate dormant DCC awakening in a murine model of CH carrying mutations in DNMT3A (KO or point mutation) and HER2+ mammary cancer. We propose our work may provide unprecedented insights into how a traceable age-related-mosaicism in the bone marrow (CH) may shape distant organs to be drive metastatic relapse.
Citation Format: Julio A. Aguirre-Ghiso, Michael Papanicolaou, Pablo Sánchez Vela, Julie Yang, Anton Safonov, Jacqueline Tao, Sebastia Franch Exposito, Vera Tiedje, Erica Dalla, Luis E. Valencia Salazar, Nicole Barth, Merve Sahin, Kevin Chen, Jinae Park, Matthew Wereski, Aishwarya Krishnan, Anthony Martinez Benitez, Ryan Ptashkin, Mark E. Robson, Elisa de Stanchina, Lior Z. Braunstein, Ahmet Zehir, Elizabeth Comen, Larry Norton, Michael Berger, Richard Koche, Pedram Razavi, Ross Levine. Age-related and organ-specific host mosaicism as a driver of dormant cancer cell awakening [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr SY34-02.
Abstract SY37-03: Leveraging the complexities of tumor associated macrophages for effective anti-cancer therapy
Authors: Guerriero Jennifer L. 
Published: April 2024

Abstract
Macrophages are innate immune cells that play a critical role in host defense and maintaining tissue homeostasis, however their infiltration into tumors is associated with disease progression and resistance to therapy. Tumor associated macrophages (TAMs) represent a significant proportion of solid tumors, including breast cancer. TAMs play a major role in tumorigenesis as they can enhance tumor cell growth, angiogenesis and metastasis. In addition, TAMs can inhibit anti-tumor responses of T cells. Our recent work has shown that removal or conversion of TAMs to an anti-tumor phenotype enhances chemo- and immuno-therapy establishing TAMs as targets for anti-cancer therapy. Using high-dimensional single-cell and multi-omics profiling of murine and human breast tumors we reveal multiple subsets of TAMs with distinct transcriptional and activity profiles that correlate with progression of disease and response to therapy. This work has revealed the complexity of TAMs and major gaps in our knowledge of the functional and phenotypic characterization of TAM subsets associated with cancer, before and after treatment. Here we will discuss the complexity of TAMs in solid tumors including characterizing TAM subsets, location, and crosstalk with neighboring cells, as well as novel TAM-modulating strategies and combinations that are likely to enhance current therapies and overcome chemo- and immuno-therapy resistance. Our findings support the idea that targeting TAMs offers great potential to enhance both chemo- and immuno-therapy.
Citation Format: Jennifer L. Guerriero. Leveraging the complexities of tumor associated macrophages for effective anti-cancer therapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 2 (Late-Breaking, Clinical Trial, and Invited Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(7_Suppl):Abstract nr SY37-03.
Identification and classification of Prakriti of human using facial features
Authors: Suguna Guttor Chickaramanna , S T Veerabhadrappa 
Published: April 2024

<p>Changes in the lifestyle of individual, several of disease has emerged due to imbalance of doshas components. Ayurveda practitioners could identify the imbalance of dosha and relate the root cause of imbalance of doshas. Analysis of dosha varies from practitioner to practitioner and it requires well practiced practitioner to identify dosha. To overcome, the darshana method was adopted to automatically identify predominant dosha using facial features such as face, eyes, nose, and mouth and skin color. Computer vision and Image processing techniques were made attempted in Ayurveda domain, for identification of predominate prakriti, age, and gender of the subject. Eye Aspect Ratio (EAR), Nose Aspect Ratio (NAR), Mouth Aspect Ratio (MAR) and skin color was computed based on Euclidean distance to identify on-live predomaint prakriti of an individual. The values of MAR ≤ 0.5,EAR ≤ 0.1,NAR ≤ 0.8 as identified as vata , 0.5 ≥ MAR ≤ 0.6, 0.1 ≥ EAR ≤ 0.2, 0.8 ≥ NAR ≤ 1 as identified as pitta and MAR ≥ 0.6,EAR ≥ 0.2 and NAR ≥ 1 as identified as kapha dosha. With the features MAR, EAR and NAR classification of predominant prakriti was carried out with an accuracy of 87.5% with support vector classifier.</p>
The rise of AI: a comprehensive research review
Authors: Rama Padmaja Chinimilli Venkata , Lakshminarayana Sadasivuni 
Published: April 2024

<p>Artificial Intelligence (AI) has emerged as a transformative force with far-reaching implications across various domains. This research review provides a comprehensive analysis of the rise of AI, examining its evolution, applications, ethical considerations, and future prospects. The study traces the historical development of AI, highlighting key milestones and technological advancements that have propelled its growth. It explores the wide-ranging applications of AI in sectors such as healthcare, finance, transportation, manufacturing, and entertainment, showcasing its impact on efficiency, decision-making, and user experiences. Ethical considerations surrounding AI, including bias, privacy, and societal implications, are thoroughly discussed. The transformative potential of AI in shaping society is examined, with insights into its effects on employment, education, governance, and societal challenges. Looking ahead, the review identifies emerging technologies and discusses challenges related to data privacy, security, and transparency. The research review concludes by emphasizing the importance of responsible and ethical development of AI, while underscoring the need for continued research and collaboration to fully harness its potential. This comprehensive review serves as a valuable resource for researchers, and practitioners seeking a holistic understanding of the rise of AI and its implications.</p>
Programmable Analogue Drawing Machines, 1952–2023
Authors: Tait Jack 
Published: April 2024

Abstract
The author discusses his work creating programmable, generative analogue drawing machines over six decades in the context of the constructivist tradition and influenced by the Bauhaus German art school and the subsequent art, science, and technology movement. During the first three decades he developed a variety of machines and from 1990 followed a more analytical approach focusing on randomness, chaos, and subjectivity in art. This progressed to the author’s PhD research program wherein he investigated innovative analogue programming systems that employed direct current motors, linkages, and cranks together with programmers, actuating motors for a variety of timed pulses. These enabled variables such as pen-lift and rotation, forward/reverse pen action, light drawing, sine waves, turntable and drum machines, and versions of X:Y plotters.
Rapidly Developing a Community- and Evidence-Based Heat Action Plan
Authors: Uejio Christopher K. , Gilbert Jane , Ahn Yoonjung , Martella Ludovica , Hu Leiqiu , Marturano Julia 
Published: April 2024

Abstract
Extreme heat contributes to 8000–12 000 excess U.S. deaths per year. Partly due to increasing summer temperatures and a renewed focus on environmental justice, local governments started new initiatives to manage and adapt to extreme heat. For example, Miami-Dade County, Florida, United States, appointed Jane Gilbert as the world’s first Chief Heat Officer. This manuscript summarizes Miami-Dade County’s preliminary efforts to build local evidence, engage the community, and rapidly respond to extreme heat. The manuscript’s goal is to expedite the translation of existing tools into mainstream extreme heat, health, and equity planning. The study generated local evidence to identify the places and periods of time with elevated heat-related illness using a statistical vulnerability and time series analysis, respectively. The places with the highest severe heat-related illness rates had hotter land surface temperatures and/or higher proportions of people who were outdoor workers, Indigenous, living in poverty or mobile homes, and households with children. “Everyday” summer conditions instead of rare heatwaves increase the risk of a heat-related death. The Chief Heat Officer convened workshops that engaged 298 unique community members on six cross-sectoral heat topics. Key recommendations included increasing multisectoral heat monitoring and risk communication, building more affordable housing, preserving and expanding greenspace, and creating heat resilience hubs. The activities culminated in a Heat Action Plan, which was completed in less than two years from the receipt of project funding.
Discrimination between benign and malignant gallbladder lesions on enhanced CT imaging using radiomics
Authors: Zhuang Ying-ying , Feng Yun , Kong Dan , Guo Li-li 
Published: April 2024

Background Gallbladder cancer is a rare but aggressive malignancy that is often diagnosed at an advanced stage and is associated with poor outcomes. Purpose To develop a radiomics model to discriminate between benign and malignant gallbladder lesions using enhanced computed tomography (CT) imaging. Material and Methods All patients had a preoperative contrast-enhanced CT scan, which was independently analyzed by two radiologists. Regions of interest were manually delineated on portal venous phase images, and radiomics features were extracted. Feature selection was performed using mRMR and LASSO methods. The patients were randomly divided into training and test groups at a ratio of 7:3. Clinical and radiomics parameters were identified in the training group, three models were constructed, and the models’ prediction accuracy and ability were evaluated using AUC and calibration curves. Results In the training group, the AUCs of the clinical model and radiomics model were 0.914 and 0.968, and that of the nomogram model was 0.980, respectively. There were statistically significant differences in diagnostic accuracy between nomograms and radiomics features ( P <0.05). There was no significant difference in diagnostic accuracy between the nomograms and clinical features ( P >0.05) or between the clinical features and radiomics features ( P >0.05). In the testing group, the AUC of the clinical model and radiomics model were 0.904 and 0.941, and that of the nomogram model was 0.948, respectively. There was no significant difference in diagnostic accuracy between the three groups ( P >0.05). Conclusion It was suggested that radiomics analysis using enhanced CT imaging can effectively discriminate between benign and malignant gallbladder lesions.
The benefit, harm, and complication of humor in social work: A qualitative inquiry
Authors: Ocean Mia , Gordon Donette A 
Published: April 2024

Summary The use of humor in social work is an underrecognized and underexplored area of practice despite its potential to heal, harm, and complicate communication. To date, very minimal scholarship has been published on humor in social work. Therefore, this qualitative study sought to examine how social workers use, perceive, and experience humor. Data were gathered from 22 participants who identified as social workers via an online survey. Data also included learning artifacts (e.g., discussion board posts, humor therapy treatment plans) from 19 graduate-level social work students who completed a humor in social work course in the northeast region of the United States. Both datasets were analyzed thematically. Findings Participants perceived social workers as using humor in clearly distinct ways, as a tool to promote generative growth, and as a weapon to harm already marginalized populations. In total, participant responses highlight the complexities of humor, including its subjective and dynamic nature, which can lead to multiple and sometimes conflicting experiences. Applications The findings suggest that social workers view humor positively, yet they engage in its use without adequate training, strategy, and reflection, sometimes violating professional ethics. Results further indicate that social workers will continue to employ humor in their professional work to the benefit and detriment of those around them. To support reflective and informed practice, additional research is needed on the use and misuse of humor in social work.
Evaluation of the low-velocity impact response of high-performance multi-axial warp-knitted flexible composites
Authors: Li Bing , Zhao Ziyu , Ma Pibo 
Published: April 2024

This article aims to investigate the dynamic behavior of high-performance multi-axial warp-knitted flexible composite materials under low-velocity impact tests through experiments and numerical simulations. In this paper, high-performance multi-axial warp-knitted flexible composites were prepared. Three different preparation processes, 175°C-5 min, 185°C-10 min, and 195°C-15 min, were designed for the multi-axial warp-knitted flexible composites. Studied the impact response of different preparation processes, initial impact energy, and punch shapes and diameters on materials. The results showed that the flexible composites prepared by various processes exhibit the same impact response curves in the impact resistance process, while the damage morphology and failure modes of the samples are different. Different initial impact energies caused multiple failure modes in the samples. The material showed penetration damage at high energy impacts and permanent depression damage at low energies. For different punch shapes, the impact resistance of materials to hemispherical punches is better than that of cylindrical punches. Numerical simulations were carried out using the finite element software ABAQUS. The custom material subroutine (VUMAT) based on the Hashin damage criterion model was implemented in the finite element program. The experimental and numerical simulation results agree regarding impact response characteristics. This paper analyzes the composite damage shapes, crack extensions caused by low-velocity impact tests and finite element simulation on multi-axial warp-knitted flexible composites. It provides a valuable reference for failure and structural optimization of multi-axial warp-knitted flexible composites for architectural applications.