bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro-elimination strategy for viral hepatitis C in western China: a hospital‑based experience
Authors: Hao Shuai , Zhang Fengna , Kong Fanjiao , Wang Ziyi , He Na 
Published: March 2024

Abstract
Objective: This study was aimed to explore a feasible, sustainable, and efficient in-hospital model of Hepatitis C micro-elimination strategy.
Methods: The strategy divided into three steps. First, the hospital inpatient database was used to retrieve 3 categories of patients at high risk for HCV infection from the October 2017-September 2020, then collected patients’ clinical data for characteristics analysis and telephone callbacks. Secondly, disease education tours in non-specialty departments led by liver specialists were conducted based on the results of the analysis of high HCV infection rate. Finally, evaluated the impact for this micro-elimination strategy at the routinely followed up periods from October 2020-May 2022.
Results: After the micro-elimination strategy conducted, the rate of HCV screening and diagnosis were significantly increased, while the lost follow-up rate were markedly decreased compared with the previous three years (all P <0.001). The results showed that patients, who were males, aged above 40, attended to gastroenterology, respiratory and critical care medicine, and neurology departments, has higher rate of HCV infection. Notably, disease education tours significantly improved the non-specialist healthcare workers’ knowledge about HCV infection, policy awareness and preventive methods (all P <0.001). In addition, 86.78% of high-risk patients demonstrated a strong willingness to accept further testing and start DAAs treatment after receiving follow-up and health education.
Conclusion: The elimination strategy of conducting non-specialist departmental Hepatitis C seminars and education in hospitals and recalling Hepatitis C patients from high-risk groups via phone calls and text messages has the advantages of simplicity, cost-effectiveness and efficiency, and plays an important role in increasing Hepatitis C screening and diagnosis rates, as well as reducing the rate of loss of high-risk patients to hospital.
Loganin ameliorates acetaminophen-induced acute liver injury via targeting TOP1MT-mediated hepatocyte apoptosis and ferroptosis
Authors: Niu Dou , Yang Yue , Yu Xiaobo , Hui Teng , Wang Meng , Wang Jigang , Xue Xiaochang , Kang Jiefang 
Published: March 2024

Abstract
Acetaminophen (APAP) overdose can cause severe liver injury, and new drugs are urgent needed for effective treatment. Small molecules in Chinese medicine have long been a treasured reservoir for drugs screening. Here, we reported that loganin (LOG), an active ingredient in Corni Fructus, exerts hepatoprotective effects as indicated by potently alleviated liver damages in APAP-induced liver injury (AILI) murine model. LOG reversed the decreased SOD, GSH and CAT levels, and reduced lipid peroxidation, ROS production, and iron overload and hence reduced apoptosis/ferroptosis of hepatocytes of AILI models, as apoptosis/ferroptosis inducers abolished, whereas their inhibitors enhanced the effect of LOG. Through the activity-based proteome profiling (ABPP) clickable alkyne-tagged LOG probe, mitochondrial topoisomerase I (TOP1MT) was captured as a direct target of LOG, which was further validated by CETSA and ITC assays. Deficiency of TOP1MT significantly compromised the effects of LOG on H2O2-induced oxidative stress cell model via regulating downstream apoptosis/ferroptosis regulators Bax, Bcl-2, NRF2, GSH, SLC7A11, and GPX4. Consistently, LOG effect was greatly eliminated in AILI mice once the endogenous hepatic TOP1MT was knocked-down by AAV-TOP1MT shRNA. Thus, TOP1MT might be a potential target for AILI treatment and LOG represents one of the most promising candidate drugs or lead compounds.
Examining the Psychometric Properties of the Turkish Version of the Proactive Coping Scale in Nursing Students: A Methodological Study
Authors: ARICA Esra OZBUDAK 
Published: March 2024

Abstract

Aim: The aim of this study was to use the “PROACTIVE Coping Scale” to adapt the scale to Turkish culture, to determine its validity and reliability in a sample of undergraduate nursing students, and to evaluate the proactive coping levels of nursing students.
Background: Proactive coping skills are very important for nursing students to cope effectively with various stressors that they may encounter both in their academic lives and in their future professional lives. There are no valid and reliable instruments for measuring the proactive coping levels of nursing students in Turkey.
Methods: The present study is a descriptive and methodological study. Research data were collected between 01.12.2023 and 01.01.2024 via face-to-face interviews. The study was completed by 272 nursing students who voluntarily agreed to participate in the study.
Results: The scale structure was confirmed with 19 items and 4 factors. The Cronbach’s alpha reliability coefficient of the PROACTIVE Coping Scale was found to be 0.816. The scale explains 67.17% of the total variance, and item correlation values vary between 0.263 and 0.650.
Conclusions: The study showed that the PROACTIVE Coping Scale is a valid and reliable instrument for evaluating the proactive coping levels of nursing students.
Installation of HbG-Makassar by base editing restores hemoglobin function: a transformative therapy for sickle cell disease
Authors: Sheehan Vivien , Kostamo Zachary , Ortega Manuel , Xu Chavonna , Feliciano Patricia , Lam Daisy , Budak Elizabeth , Winton Valerie , Jenkins Rebecca , Menon Archita , Goldsborough Kennedy , Hernandez Britney , Kanne Celeste , Evans Erica , Zgodny Jordan , Zhang Yankai , Darazim Jawa , Patel Ashwin , Pendergast Michael , Hartigan Adam , Ciaramella Giuseppe , Chu S. Haihua , Lee Seung-Joo 
Published: March 2024

Abstract

Adenine base editing offers a viable gene-based therapy for sickle cell disease (SCD), converting sickle hemoglobin (HbS, βΕ6V) to G-Makassar hemoglobin (HbG, βE6A), a naturally occurring, non-pathogenic variant. However, HbG functionality alone and with HbS has been largely uncharacterized. We present a mouse model used to characterize purified HbG-Makassar as well as HbGG and HbGS red blood cell function. Purified HbG-Makassar behaves as a functional hemoglobin, including no polymerization under hypoxia. Structural characterization of oxy and deoxy states of HbG-Makassar showed no change in the topology of the hemoglobin fold with the βΕ6Α mutation. Red blood cell function assays, sickling propensity under hypoxia, blood counts, and mitochondrial retention measures place HbGS RBCs as intermediate in severity between HbAS and HbSS, organ function was comparable to HbAS. HbGG resembled HbAA for most metrics. Taken together our results suggest direct correction of HbS to HbG-Makassar could provide a transformative therapy for SCD.
Ensuring nutrient-enriched wheat varities for trace elements through screening under different agro- ecological conditions for food security.
Authors: Mangan BAKHT NISA , Lashari Muhammad saddique , KALERI ASIF ALI , Baloch Nazia 
Published: March 2024

Abstract
In Asia, Pakistan’s children are more stunted and suffered from malnutrition as compared to other underdeveloped counties. Focusing the importance of wheat and malnutrition issue in Pakistan, a Field and Lab study was undertaken on 60 wheat varieties during 2017–2018 and 2018–2019 to investigate the variability and availability for trace elements such as Zn, Fe, Se, Mn, Cu under three agro-ecological locations. All three locations come under different agro-ecological conditions due to their weather, soil and morphological conditions. According to the result the tested sixty wheat varieties were ranged higher from 30.33mg kg− 1 to 51.96 mg kg− 1grain Fe, from 16.03 to 54.93 mg kg− 1grain Zn, 19.95–45.97 mg kg− 1 grain Mn, 3.16–8.98 mg kg− 1 grain Cu and 46.0-307.42µg kg− 1grains Se concentration at Hyderabad and Mirpurkhas location during both studies years as compared to Thatta location. According to the findings, out of 60 analyzed varieties, 06 showed capability to accumulate maximum concentration of for elements and among all six-supper varieties, variety IV-03 showed superiority over all by producing maximum trace element content not only at Hyderabad and Mirpurkhas but also at Thatta. By adopting such type of varieties in daily diet, Pakistan can overcome health and growth issues.
A Hollow-Tube-Like Hydrospongel for Magnetothermal-Chemo-Chemodynamic Therapies in Advanced Colorectal Cancer Patients
Authors: Wang Zhenning , Wu Tao , Li Tenghui , Zhang Chengzhi , Tian Yu , Li Hao , He Yuxin , Yan Xu , Zhao Junhua , Gong Tianxing 
Published: March 2024

Abstract

In advanced-stage colorectal cancer (CRC) treatments, preserving anal function presents significant challenges. This study introduces a novel hollow-tube-like hydrospongel (HTHSG), offering a groundbreaking approach to neoadjuvant therapy. Constructed from cellulose nanofibers crosslinked with Fe3O4@PDA nanoparticles, the HTHSG combines the rapid swelling characteristics of sponges (~ 10 seconds) with the robust fracture strength of hydrogels (> 250 kPa). Its unique design ensures stability and location retention during simulated bowel movements, facilitating precise, localized delivery of the chemotherapeutic agent 5-FU directly to the tumor site. Beyond conventional chemotherapy, the HTHSG leverages electromagnetic induction for targeted thermal ablation and chemodynamic therapy, significantly minimizing the collateral damage to healthy tissues typically associated with Fe-mediated Fenton reactions. Comparative analyses using cell-derived and patient-derived xenograft models highlight the HTHSG's superior efficacy in tumor reduction based on traditional neoadjuvant therapies. These findings suggest the HTHSG's potential to redefine standard pre-surgical treatment regimens for advanced-stage CRC patients, marking a significant advancement over existing chemotherapy approaches.
Comparison of Risk Allele Frequencies for Colorectal Cancer-Associated Single Nucleotide Polymorphisms Among Different Ethnic Groups Running title: CRC Risk Allele Frequency Across Populations
Authors: Park Suyoung , Lee Kyung-Ju , Shin Hyun-Tae , Ha Hyerim 
Published: March 2024

Abstract

Background
Colorectal cancer is the second leading cause of cancer-related fatalities. Recent genome-wide association studies (GWAS) identified genetic loci implicated in colorectal cancer susceptibility. This study aimed to uncover genetic factors influencing colorectal cancer rates among different populations, particularly in East Asia where prevalence is increasing.
Methods
A total of 423 colorectal cancer-associated single-nucleotide polymorphisms (SNPs) in the GWAS catalog were examined. Allele frequencies of these SNPs were analyzed across various populations. Data on allele frequencies at the population level were sourced from the 1000 Genomes Project and the Korean Reference Genome Database. We used Fisher's exact test to determine if the effect allele at a specific SNP was significantly overrepresented or underrepresented.
Results
Allele frequencies varied across populations. East Asians and Koreans showed similar patterns, distinct from Europeans. They also displayed higher genetic risk scores, correlating with colorectal cancer incidence.
Conclusions
Disparities in allele frequencies of colorectal cancer -associated SNPs were identified across populations, particularly between Koreans and other ethnic groups. These distinctions may help elucidate the heightened colorectal cancer prevalence, primarily among Koreans and East Asians. Our study underscores that genetic dissimilarities can, in part, account for the variance in colorectal cancer occurrence among diverse population subgroups.
The mediating roles of loneliness at work and burnout in the relationship between career adaptability, organizational commitment, and job satisfaction among teachers
Authors: Kara Ahmet , Kayışdağ Erman , Bek Hafız 
Published: March 2024

Abstract
This research examines the mediating roles of loneliness at work and burnout in the relationship between career adaptability, organizational commitment, and job satisfaction among teachers. The analysis was conducted with 256 teachers. Data were collected with the Organızatıonal Commıtment Scale, Career Adaptabilities Scale, Loneliness at Work Scale, Burnout Measure, and Job Satisfaction Scale. Data were analyzed with a two-stage structural equation modeling technique. According to the research findings, it has been proven that the mediatory effects of loneliness at work and burnout are significant in the relationship between organizational commitment and job satisfaction.
Klebsiella Pneumoniae-Derived Outer Membrane Vesicles Impair Endothelial Function by inhibiting SIRT1
Authors: Li Xinxin , Cui Jinghua , Ding Zanbo , Tian Ziyan , Liu Yang , Zhao Wen , Chen Xueying , Li Xinwei , Yuan Jing , Zhang Huina 
Published: March 2024

Abstract

Background
The potential role of Klebsiella pneumoniae (K.pn) in hypertension development has been emphasized, although the specific mechanisms have not been well understood. Outer membrane vesicles (OMVs) released by gram-negative bacteria modulate host cell functions through delivery of bacterial moleculars to host cells. Endothelial dysfunction is an important early event in the pathogenesis of hypertension, yet the impact of K.pn-secreted OMVs (K.pn OMVs) on endothelial function remains unclear. This study aims to investigate the effects of K.pn OMVs on endothelial function and uncover the underlying mechanisms.
Methods
K.pn OMVs were purified from the bacterial culture medium using ultracentrifugation and characterized by transmission electron microscopy nanoparticle tracking analysis, and OMV marker expression. Endothelium-dependent relaxation was measure using wire myograph following in vivo or ex vivo treatment with K.pn OMVs. The superoxide anion production was measured by confocal microscopy and HUVECs senescence was assessed according to SA-β-gal activity. SIRT1 ovexpression or activator was utilized to investigate the underlying mechanisms.
Results
Our data showed that K.pn markedly impaired acetylcholine-induced endothelium-dependent relaxation, and elevated superoxide anion production in endothelial cells in vivo. Similarly, in vivo and ex vivo studies showed that K.pn OMVs caused significant endothelial dysfunction, provoked endothelial and enhanced blood pressure. Further examination revealed that K.pn OMVs reduced the levels of SIRT1 and p-eNOS, along with an increase in the levels of NOX2, COX-2, ET-1 and p53 in endothelial cells. Notably, overexpression or activation of SIRT1 attenuated the adverse effects and protein changes caused by K.pn OMVs on endothelial cells
Conclusion
This study reveals a novel role of K.pn OMVs in endothelial dysfunction and dissects the relevant mechanism involved in this process, which will help to establish a comprehensive understanding of K.pn OMVs in endothelial dysfunction and hypertension from a new scope.
Factors associated with full COVID-19 vaccination among persons living with diabetes mellitus in Uganda – A Cross-sectional Study
Authors: Senoga Umar , Guwatudde David , Isunju John Bosco , Ojiambo Kevin Ouma 
Published: March 2024

Abstract

Background:Diabetes mellitus (DM) is associated with severe coronavirus disease (COVID-19) outcomes, including death. COVID-19 vaccination is the mainstay for preventing infection and severe disease in the general population, but most importantly, among high-risk populations such as persons with DM. However, while COVID-19 vaccination uptake in the general population is regularly tracked, information on vaccination behavior specific to persons with DM is scarce.This study sought to identify factors associated with the uptake of full COVID-19 vaccination among persons living with DM at Kiruddu National Referral Hospital (KRH).Methods:We used a cross-sectional study design to identify factors associated with receiving a full COVID-19 vaccination among persons with DM attending Kiruddu National Referral Hospital between March 08 and May 25, 2023. We used a systematic sampling technique to select a random sample of 340 participants for whom an interviewer-administered questionnaire was administered. We used a modified Poisson regression model to identify the factors associated with full COVID-19 vaccination and adjusted prevalence ratios with their 95% confidence intervals are presented. The data were analyzed in STATA version 14.0.Results:A total of 340 participants, 75% (255/340) of whom were female, completed the interview. The mean age of the participants was 52 years (±12 years) and the median duration of diabetes mellitus was 5 years (±7.3 years). Overall, 195/340 participants (57.35%) completed the last dose in the primary series of a COVID-19 vaccine. The likelihood of receiving full vaccination was higher when a health provider advised one to receive a vaccine [aPR=1.91 (95% CI 1.2-3.02)] when one reported having a comorbidity [aPR=1.26 (95% CI: 1.06-1.53)], and when one had a strong perceived benefit of vaccination [aPR=1.76 (95% CI: 1.23-2.53)]. However, having a strong perceived barrier to vaccination was negatively associated with the likelihood of being fully vaccinated [aPR=0.71 (95% CI: 0.6-0.84)].Conclusion:Approximately 43% of persons living with DM in Uganda have incomplete COVID-19 vaccination. The Ugandan Ministry of Health (MoH) needs to implement a provider-initiated vaccination strategy in diabetic clinics using the healthcare providers who regularly attend to DM patients to boost their uptake of full COVID-19 vaccination.
Research on the large-format splicing method for stamping-type digital light processing 3D printing
Authors: Liu Lei , Li Jian , Zhao Wen , Guo Yanling , Wang Yangwei , Jiang Zaixing , Dong Jidong 
Published: March 2024

Abstract

In response to the strong demand for the development of large-format digital light processing (DLP) 3D printing technology, the use of multiple ultraviolet (UV) fixed splicing poses challenges such as high costs and limitations in print size. Therefore, this paper proposes a stamping-type DLP surface splicing 3D printing method, namely, achieving the large-format DLP 3D printing effect through the intermittent movement of a single UV machine along two axial directions. To address the splicing issues inherent in the stamping-type DLP 3D printing surface printing method, we have divided the splicing areas and deduced the principles governing energy distribution at the seams of adjacent printed areas. By introducing a normal distribution process and combining it with the projection function and energy distribution principles, a method for the gradient energy distribution of overlapping splicing zones is proposed. The effectiveness of this method is experimentally validated, demonstrating that the minimum size error of printed pieces is -0.0591, the minimum error in surface roughness at splicing and non-splicing areas is 0.13%, the tensile strength is comparable to that of integral curing, and the overall hardness distribution is relatively uniform.
Inpatient preference among patients with multiple chronic conditions in China: a discrete choice experiment
Authors: Wang Yueqin , Chen Yuehua , Xiao Ling , Su Qingling , Huang Xiaoyin , Wang Weikang , Li Wanxin , Du Shanshan , Liu Wenbin , Ye Weimin 
Published: March 2024

Abstract

Background
Multiple chronic conditions (MCC) have become a leading cause of hypovitalism and death among the population. Although the role of chronic disease management has been strengthened for primary health institutions, patients still have a preference for a higher level of inpatient service, leading to a considerable hindrance in the efficient utilization of healthcare resources. Thus, this study aimed to identify factors that affected MCC patients' inpatient preference and the extent to which these factors impact their decisions, guiding for inpatient service utilization among MCC patients.
Methods
Five attributes (institutional scale, waiting time for hospital admission, with or without acquaintances, time from residence to hospital, out-of-pocket expenses per time) were identified to estimate inpatient choice for MCC patients through a discrete choice experiment. Partial factor analysis was performed to generate selection sets. Data were collected from MCC patients between 35 and 75 years old, in Fuqing, Fujian Province. A mixed logit model was performed to analyze MCC patients' preferences for each attribute. Willingness to pay was estimated by regression coefficients, and subgroup analysis was conducted based on the patient’s demographic characteristics and overall perceived disease severity.
Results
Totally 504 valid questionnaires were included in the analysis. MCC patients preferred to have a shorter time from their residence to the hospital (β = 0.7602, p < 0.001), large provincial and municipal tertiary hospitals (β = 0.2635, p < 0.001), and have beds available on the day (β = 0.1962, p = 0.0028). Out-of-pocket expenses per time (β=-0.0006, p < 0.001) are a negative predictor of patients' inpatient preference. Additionally, Patients were willing to pay ¥1253, ¥434, and ¥323 for higher institutional scale, shorter waiting time, and shorter time from residence-to-hospital, respectively. The findings of subgroup analysis indicated that some demographic characteristics (age, gender, education and total household income) and overall perceived disease severity also influence MCC patients' inpatient preferences.
Conclusion
This study provides evidence on the inpatient preferences among MCC patients. To better meet patients’ needs, it is recommended to improve the geographical accessibility of medical and health services, strengthen the service capacity of medical personnel in county and community-level medical institutions, streamline the inpatient diagnosis and treatment process, and so on.
Private provider practices and incentives for hypertension management in rural and peri-urban Telangana, India– a qualitative study.
Authors: Gupte Samriddhi S , Sachdeva Ashish , Kabra Aman , Singh Bhanu Pratap , Krishna Ashish , Pathni Anupam Khungar , Sharma Bhawna , Moran Andrew , Mamindla Amarendar Reddy , Kannuri Nanda Kishore , Deo Sarang 
Published: March 2024

Abstract

Hypertension poses a critical threat to health in India, being the predominant risk factor for mortality and disability. With over 70% of outpatient care being provided by the private sector, our study investigated the practices and incentives of private health providers in screening, diagnosing, treating, monitoring, and counseling hypertension patients in rural and peri-urban India. Conducted from April 2020 to February 2021, the qualitative study involved 46 participants, including various healthcare professionals and patients in three Telangana state districts. Analysis revealed a lack of recognition of hypertension's gravity among private providers in these areas, leading to inconsistent screening practices and varied diagnostic thresholds, particularly among rural medical practitioners. Both formal and informal providers lacked standardized protocols and follow-up mechanisms, with limited technical knowledge about hypertension observed, especially among rural practitioners who were often the first point of contact. Drug effectiveness, and incentives from pharmaceutical sales representatives influenced prescribing practices. Diagnostic labs also offered financial incentives for patient referrals. Thus, aligning providers' objectives with patient preferences and public health goals is crucial. To encourage evidence-based hypertension care, the government and NGOs could implement strategies such as tailored incentives, financial rewards, tax benefits, accreditation, and recognition for private healthcare providers. Professional bodies in the private sector should establish programs emphasizing quality assurance and certifications. Future research should focus on designing and testing new models for private sector hypertension service delivery, coupled with targeted interventions to enhance care in rural and peri-urban settings.