bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic profiling of KATP channel‐deficient hypertensive heart maps risk for maladaptive cardiomyopathic outcome
Authors: Zlatkovic Jelena , Arrell D. Kent , Kane Garvan C. , Miki Takashi , Seino Susumu , Terzic Andre 
Published: February 2009

AbstractKCNJ11 null mutants, lacking Kir6.2 ATP‐sensitive K+ (KATP) channels, exhibit a marked susceptibility towards hypertension (HTN)‐induced heart failure. To gain insight into the molecular alterations induced by knockout of this metabolic sensor under hemodynamic stress, wild‐type (WT) and Kir6.2 knockout (Kir6.2‐KO) cardiac proteomes were profiled by comparative 2‐DE and Orbitrap MS. Despite equivalent systemic HTN produced by chronic hyperaldosteronism, 114 unique proteins were altered in Kir6.2‐KO compared to WT hearts. Bioinformatic analysis linked the primary biological function of the KATP channel‐dependent protein cohort to energetic metabolism (64% of proteins), followed by signaling infrastructure (36%) including oxidoreductases, stress‐related chaperones, processes supporting protein degradation, transcription and translation, and cytostructure. Mapped protein–protein relationships authenticated the primary impact on metabolic pathways, delineating the KATP channel‐dependent subproteome within a nonstochastic network. Iterative systems interrogation of the proteomic web prioritized heart‐specific adverse effects, i.e., “Cardiac Damage”, “Cardiac Enlargement”, and “Cardiac Fibrosis”, exposing a predisposition for the development of cardiomyopathic traits in the hypertensive Kir6.2‐KO. Validating this maladaptive forecast, phenotyping documented an aggravated myocardial contractile performance, a massive interstitial fibrosis and an exaggerated left ventricular size, all prognostic indices of poor outcome. Thus, Kir6.2 ablation engenders unfavorable proteomic remodeling in hypertensive hearts, providing a composite molecular substrate for pathologic stress‐associated cardiovascular disease.
Complex micelles formed from two diblock copolymers for applications in controlled drug release
Authors: Li Guiying , Guo Lei , Ma Songmei , Liu Junshen 
Published: February 2009

AbstractDouble‐responsive core‐shell‐corona complex micelles for applications in drug release were formed from self‐assembly of two diblock copolymers PtBA‐b‐ PNIPAM and PtBA‐b‐P4VP. The two diblock copolymers coaggregated into core‐shell complex micelles in acidic water with the hydrophobic PtBA blocks as the common core and soluble PNIPAM/P4VP blocks as the mixed shell. Increasing temperature or pH value, the micelles converted into core‐shell‐corona micelles because of the collapse of PNIPAM or P4VP blocks as the inner shell and soluble P4VP or PNIPAM chains stretching outside as the outer corona. The anti‐inflammation drug naproxen (NAP) was loaded as the model drug in micelles in acidic water and released because of the ionization of NAP in alkaline solutions. Compared with pure core‐shell micelles, release of NAP from core‐shell‐corona complex micelles avoided the burst diffusion and the release rate is more easily controlled by tuning the composition of the mixtures or by adjusting the pH of the medium. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 1804–1810, 2009
Synthesis and properties of carbamate‐ and amine‐containing poly(phenylacetylenes)
Authors: Saeed Irfan , Khan Fareha Zafar , Shiotsuki Masashi , Masuda Toshio 
Published: February 2009

AbstractThe 3‐ and 4‐aminophenylacetylenes protected by t‐butoxycarbonyl (t‐Boc) and 9‐fluorenylmethoxycarbonyl (Fmoc) groups (3a–6a) were synthesized and polymerized using [(nbd)RhCl]2 (1) and [(nbd)Rh+‐η6‐PhB−Ph3] (2) catalysts. The t‐Boc‐containing polymers [poly(3a) and poly(4a)] were obtained in high yield (82–91%). Among the Fmoc‐protected monomers, the para‐derivative polymerized well [poly(6a); yield = 85–94%], whereas its meta‐substituted analogue did not afford high molecular weight polymer in good yield [poly(5a); yield = 10–15%]. The use of KN(SiMe3)2 as a cocatalyst in conjunction with 1 led to a dramatic increase in the molecular weight of the polymers. The acid‐ and base‐catalyzed removal of the t‐Boc and the Fmoc groups, respectively, generated primary amine‐containing polymers [poly(3b)–poly(6b)] which cannot be obtained directly by the polymerization of the corresponding monomers. The solubility characteristics of the polymers bearing protected amino groups were quite different from those of the unprotected ones, the former being soluble in polar solvents, whereas the latter displayed poor solubility even in polar protic or highly polar aprotic solvents. The attempts to accomplish the free‐standing membrane fabrication by solution casting were successful only for poly(3a), and an augmentation in the gas permeability and CO2/N2 permselectivity was discerned in comparison with the unsubstituted poly(phenylacetylene) and poly(m‐t‐butyldimethylsiloxyphenylacetylene). © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 1853–1863, 2009
Type of social support matters for prediction of posttraumatic growth among cancer survivors
Authors: Schroevers Maya J. , Helgeson Vicki S. , Sanderman Robbert , Ranchor Adelita V. 
Published: February 2009

AbstractObjective: Previous research in people with cancer on social support and psychological well‐being has mainly focused on the short‐term negative outcomes of adjustment. Little is known about the role of social support in the experience of positive outcomes in the long term. This study examined the relation between emotional support in the period following diagnosis and the experience of positive consequences of the illness, so called posttraumatic growth, at 8 years after diagnosis. We focused on three distinct types of emotional support: perceived availability, actual received, and dissatisfaction with received emotional support.Methods: This longitudinal study was conducted in a sample of 206 long‐term cancer survivors. Social support was assessed with the Social Support List (SSL) at 3 months and 8 years after diagnosis. Positive consequences of the illness were assessed with the Silver Lining Questionnaire (SLQ) at 8 years after diagnosis. Correlation‐ and regression analyses were used to examine the associations of initial levels of emotional support with the long‐term report of posttraumatic growth.Results: Regression analyses showed that more received emotional support at 3 months after diagnosis significantly predicted a greater experience of positive consequences of the illness at 8 years after diagnosis. This association remained significant, when controlling for concurrent levels of emotional support at 8 years after diagnosis.Conclusions: The findings suggest that getting support from family and friends, characterized by reassuring, comforting, and problem‐solving, in the period following diagnosis is an important resource that may help cancer survivors to find positive meaning in the cancer experience. Copyright © 2009 John Wiley & Sons, Ltd.
Proteomics analysis of liver pathological calcification suggests a role for the IQ motif containing GTPase activating protein 1 in myofibroblast function
Authors: Kalantari Fariba , Auguste Patrick , Ziafazeli Tahereh , Tzimas George , Malmström Lars , Bioulac‐Sage Paulette , Boismenu Daniel , Vali Hojatollah , Chevet Eric 
Published: February 2009

AbstractTo date the cellular and molecular mechanisms by which liver pathological calcifications occur and are regulated are poorly investigated. To study the mechanisms linked to their appearance, we performed a proteomics analysis of calcified liver samples. To this end, human liver biopsies collected in noncalcified (N), precalcified (P), and calcified (C) areas of the liver were subjected to weak ion exchange chromatography, SDS‐PAGE, and LC‐ESI MS/MS analyses. As we previously demonstrated that alpha‐smooth muscle actin (α‐SMA) expressing myofibroblasts were involved in liver pathological calcification, we performed a targeted analysis of actin cytoskeleton remodeling‐related proteins. This revealed dramatic changes in protein expression patterns in the periphery of the calcified areas. More particularly, we found that IQGAP1 and IQGAP2 proteins were subjected to major expression changes. We show that IQGAP1 expression within P and C areas of the liver correlates with the high abundance of myofibroblasts and that IQGAP1 is specifically expressed in these cells. In addition, we find that IQGAP1 is part of a protein complex including β‐catenin and Rac1 mainly in P and C regions of the liver. These results suggest that IQGAP1 may play a critical role in the regulation of cytoskeleton remodeling in liver myofibroblasts in response to liver injury and consequently impact on their function.
Quantitative nuclear proteomics reveals new phenotypes altered in lymphoblastoid cells
Authors: Brennan Paul , Shore Angharad M. , Clement Mathew , Hewamana Saman , Jones Catrin M. , Giles Peter , Fegan Christopher , Pepper Christopher , Brewis Ian A. 
Published: February 2009

AbstractB‐lymphocytes are essential for the production of antibodies to fight pathogens and are the cells of origin in 95% of human lymphomas. During their activation, and immortalisation by Epstein–Barr virus (EBV) which contributes to human cancers, B‐lymphocytes undergo dramatic changes in cell size and protein content. This study was initiated to compare the proteome of two B‐cell lines, from the same individual, that reflect different patterns of activation, one is EBV negative and the other is EBV positive. Using isobaric tags, LC‐MALDI TOF‐TOF and subcellular fractionation, we quantified 499 proteins from B‐cells. From a detergent lysed protein extract, we identified 34 proteins that were differentially expressed in EBV‐immortalised B‐cells. By analysing a nuclear extract, we identified a further 29 differentially expressed proteins with only four proteins shared between the two extracts, illustrating the benefit of subcellular fractionation. This analysis has identified proteins involved in the cytoskeletal phenotype of activated B‐cells and the increased antigen recognition in EBV‐immortalised cells. Importantly, we have also identified new regulators of transcription and changes in ribonuclear proteins that may contribute to the increased cell size and immortalisation of lymphoblastoid cells.
Assessing wind profile effects on the global atmospheric torque
Authors: Miranda P. M. A. , Martins J. P. A. , Teixeira M. A. C. 
Published: February 2009

AbstractThe impact of a new approach to the evaluation of surface gravity wave drag (GWD) is assessed. This approach uses linear theory, but incorporates the effects of wind profile shear and curvature, by means of a second‐order WKB approximation. While the theory predicts the possibility of either drag enhancement or reduction, depending on the wind profile, results obtained with the ERA‐40 reanalysis data clearly indicate the predominance of local drag enhancement. However, the global impact of shear on the atmospheric axial GWD torque comes mostly from regions with predominantly easterly flow, contributing to a slight reduction of the bias found in different studies of the global angular momentum budget. The relative correction due to shear on linear GWD is found not to depend too strongly on the levels chosen for the computation of the low‐level wind derivatives. Copyright © 2009 Royal Meteorological Society
Are liquid chromatography/electrospray tandem quadrupole fragmentation ratios unequivocal confirmation criteria?
Authors: Kaufmann Anton , Butcher Patrick , Maden Kathryn , Widmer Mirjam , Giles Kevin , Uría Diana 
Published: February 2009

AbstractMultiple reaction monitoring (MRM) ratios as provided by tandem mass spectrometers are used to confirm positive residue findings (e.g. veterinary drugs or pesticides). The Commission Decision 2002/657/EEC defines tolerance levels for MRM ratios, which are intended to prevent the reporting of false positives. This paper reports findings where blank sample extracts have been spiked by a drug (difloxacin) and the corresponding measured MRM ratios significantly deviated from MRM ratios observed in matrix‐free solution. The observation was explained by the formation of two different [M+H]+ analyte ions within the electrospray ionization (ESI) interface. These two ions vary only by the site of analyte protonation. Since they are isobaric, they are equally transmitted through the first quadrupole, but are differently fragmented in the collision chamber. The existence of two isobaric ions was deduced by statistical data and the observation of a doubly charged analyte ion. It was hypothesized that the combined presence of [M+H]+ and [M+2H]2+ implies the existence of two different singly charged ion species differing only by the site of protonation. Low‐ and high‐energy interface‐induced fragmentation was performed on the samples. The surviving precursor ion population was mass selected and again fragmented in the collision chamber. Equal product ion spectra would be expected. However, very different product ion spectra were observed for the two interface regimes. This is consistent with the assumption that the two postulated isobaric precursor ions show different stability in the interface. Hence the abundance ratio among the two types of surviving precursor ions will shift and change the resulting product ion spectra. The existence of the postulated singly charged ions with multiple chargeable sites was finally confirmed by successful ion mobility separation. Copyright © 2009 John Wiley & Sons, Ltd.
Matrix Metalloproteinase 1 Is Necessary for the Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Human Glioma
Authors: Ho Ivy A. W. , Chan Kelly Y. W. , Ng Wai-Hoe , Guo Chang M. , Hui Kam M. , Cheang Philip , Lam Paula Y. P. 
Published: February 2009

AbstractHuman mesenchymal stem cells (MSCs) have increasingly been used as cellular vectors for the delivery of therapeutic genes to tumors. However, the precise mechanism of mobilization remains poorly defined. In this study, MSCs that expressed similar cell surface markers and exhibited multilineage differentiation potentials were isolated from various donors. Interestingly, different MSC isolates displayed differential migration ability toward human glioma cells. We hypothesized that distinct molecular signals may be involved in the varied tumor tropisms exhibited by different MSC isolates. To test this hypothesis, gene expression profiles of tumor-trophic MSCs were compared with those of non–tumor-trophic MSCs. Among the various differentially regulated genes, matrix metalloproteinase one (MMP1) gene expression and its protein activities were enhanced by 27-fold and 21-fold, respectively, in highly migrating MSCs compared with poorly migrating MSCs. By contrast, there was no change in the transcriptional levels of other MMPs. Functional inactivation of MMP1 abrogated the migratory potential of MSCs toward glioma-conditioned medium. Conversely, the nonmigratory phenotype of poorly migrating MSC could be rescued in the presence of either recombinant MMP1 or conditioned medium from the highly migrating MSCs. Ectopic expression of MMP1 in these poorly migrating cells also rendered the cells responsive to the signaling cues from the glioma cells in vivo. However, blocking the interaction of MMP1 and its cognate receptor PAR1 effectively diminished the migratory ability of MSCs. Taken together, this study provides, for the first time, supporting evidence that MMP1 is critically involved in the migration capacity of MSCs, acting through the MMP1/PAR1 axis.Disclosure of potential conflicts of interest is found at the end of this article.
Identification of a Stroma-Mediated Wnt/β-Catenin Signal Promoting Self-Renewal of Hematopoietic Stem Cells in the Stem Cell Niche
Authors: Kim Jin-A , Kang Young-Ju , Park Gyeongsin , Kim Myungshin , Park Young-Ok , Kim Hanjun , Leem Sun-Hee , Chu In-Sun , Lee Jun-Seong , Jho Eek-Hoon , Oh Il-Hoan 
Published: February 2009

Abstract
With contrasting observations on the effects of β-catenin on hematopoietic stem cells (HSCs), the precise role of Wnt/β-catenin signals on HSC regulation remains unclear. Here, we show a distinct mode of Wnt/β-catenin signal that can regulate HSCs in a stroma-dependent manner. Stabilization of β-catenin in the bone marrow stromal cells promoted maintenance and self-renewal of HSCs in a contact-dependent manner, whereas direct stabilization in hematopoietic cells caused loss of HSCs. Interestingly, canonical Wnt receptors and β-catenin accumulation were predominantly enriched in the stromal rather than the hematopoietic compartment of bone marrows. Moreover, the active form of β-catenin accumulated selectively in the trabecular endosteum in “Wnt 3a-stimulated” or “irradiation-stressed,” but not in “steady-state” marrows. Notably, notch ligands were induced in Wnt/β-catenin activated bone marrow stroma and downstream notch signal activation was seen in the HSCs in contact with the activated stroma. Taken together, Wnt/β-catenin activated stroma and their cross-talk with HSCs may function as a physiologically regulated microenvironmental cue for HSC self-renewal in the stem cell niche.
Disclosure of potential conflicts of interest is found at the end of this article.
Multitracer assessment of dopamine function after transplantation of embryonic stem cell‐derived neural stem cells in a primate model of Parkinson's disease
Authors: Muramatsu Shin‐Ichi , Okuno Tsuyoshi , Suzuki Yutaka , Nakayama Takashi , Kakiuchi Takeharu , Takino Naomi , Iida Asako , Ono Fumiko , Terao Keiji , Inoue Nobuo , Nakano Imaharu , Kondo Yasushi , Tsukada Hideo 
Published: February 2009

AbstractThe ability of primate embryonic stem (ES) cells to differentiate into dopamine (DA)‐synthesizing neurons has raised hopes of creating novel cell therapies for Parkinson's disease (PD). As the primary purpose of cell transplantation in PD is restoration of dopaminergic neurotransmission in the striatum, in vivo assessment of DA function after grafting is necessary to achieve better therapeutic effects. A chronic model of PD was produced in two cynomolgus monkeys (M‐1 and M‐2) by systemic administration of neurotoxin. Neural stem cells (NSCs) derived from cynomolgus ES cells were implanted unilaterally in the putamen. To evaluate DA‐specific functions, we used multiple [11C]‐labeled positron emission tomography (PET) tracers, including [β‐11C]L‐3,4‐dihydroxyphenylalanine (L‐[β‐11C]DOPA, DA precursor ligand), [11C]‐2β‐carbomethoxy‐3β‐(4‐fluorophenyl)tropane ([11C]β‐CFT, DA transporter ligand) and [11C]raclopride (D2 receptor ligand). At 12 weeks after grafting NSCs, PET demonstrated significantly increased uptake of L‐[β‐11C]DOPA (M‐1:41%, M‐2:61%) and [11C]β‐CFT (M‐1:31%, M‐2:36%) uptake in the grafted putamen. In addition, methamphetamine challenge in M‐2 induced reduced [11C]raclopride binding (16%) in the transplanted putamen, suggesting release of DA. These results show that transplantation of NSCs derived from cynomolgus monkey ES cells can restore DA function in the putamen of a primate model of PD. PET with multitracers is useful for functional studies in developing cell‐based therapies against PD. Synapse 63: 541‐548, 2009. © 2009 Wiley‐Liss, Inc.
A novel portable multimedia QoS monitor: independent and high efficiency
Authors: Zhou Qingguo , Bai Shuwei , Hu Bin , Guire Nicholas MC , Li Lian 
Published: February 2009

AbstractIn this paper, a novel multimedia QoS monitor, p3m (Portable MultiMedia Monitor), is presented. The main function of p3m is data package analysis and statistics. Breaking through the conventional method, this new QoS monitor moves jobs into kernel space, by which to meet the real‐time requirements of multimedia services. With the development of broadband Internet, more and more heterogeneous multimedia services and protocols have been widely deployed. As known, there are two traditional QoS monitors. One is server‐based, depending on the specific multimedia server, such as Helix. Another is tcpdump‐based, e.g. mmdump, which can measure the nature of multimedia traffic merely. However, it is still difficult to monitor the QoS of multimedia networks by these two traditional strategies. To resolve the problems, our new system adopts MMPM (MultiMedia Packages Management) mechanism which can make monitor tool become server‐independent and monitor the multimedia packets by preference, thus greatly reducing resource requirements with offering high performance. This paper describes the design and implementation of p3m and demonstrates one instance of its utility in monitoring the QoS of RTSP with Helix Server. The evaluation of p3m is also presented in this paper. Copyright © 2009 John Wiley & Sons, Ltd.
Dy2Te3O9: Der erste Vertreter von Lanthanoid(III)‐Oxotelluraten der Zusammensetzung M2Te3O9
Authors: Meier Steffen F. , Höss Patrick , Schleid Thomas 
Published: February 2009

AbstractDy2Te3O9: The First Representative of Lanthanoid(III)‐Oxotellurates(IV) with the Composition M2Te3O9Single crystals of Dy2Te3O9 could be obtained by reaction of Dy2O3 with TeO2 in a 1 : 4 molar ratio as first representative of the formula type M2Te3O9. In this oxotellurate(IV), the lanthanoid(III) cations are hepta‐ (capped trigonal prism: Dy4) and octacoordinated (bicapped trigonal prisms: Dy1–Dy3) by oxygen atoms, respectively. These dysprosium–oxygen polyhedra build up layers parallel to the (100) plane by sharing common edges as dominating feature of the crystal structure. Because of the proportionally higher Dy2O3 moiety in Dy2Te3O9 (Dy2O3 : TeO2 =1 : 3), these ∞2{[Dy4O17]22–} sheets appear more bulky and corrugated than the almost planar ∞2{[Dy2O11]16–} layers in Dy2Te4O11 (Dy2O3 : TeO2 = 1 : 4). In spite of its rather complex constitution, the tellurium–oxygen partial structure in Dy2Te3O9 shows remarkably different motifs. The six crystallographically independent Te4+ cations are each surrounded by three oxygen atoms, which form together with the lone‐pair electrons [TeO3]2– ions of ψ1‐tetrahedral shape. However, three of them show higher coordination numbers such as CN = 3+3 (Te1) and CN = 3+2 (Te2 and Te3) by strong secondary Te···O interactions with adjacent oxotellurate(IV) units, whilst the other three (Te4–Te6: CN = 3) have no secondary contacts at all. The typical 3+1‐coordination of Te4+ as in Dy2Te4O11 and generally common in other rare‐earth metal(III) oxotellurates(IV) does not occur. Also oxoditellurate(IV) units ([Te2O5]2–) as in Dy2Te4O11 or even more condensed ones ([Te3O8]4–) as in Dy2Te5O13 cannot be observed in Dy2Te3O9, owing to its proportionally smaller TeO2 moiety. Five of the [TeO3]2– ions group to screw‐like strands along the [010] direction according to ∞1{[Te5O15]10–} through strong secondary Te···O contacts, whereas the remaining [(Te6)O3]2– units reside isolated within channelsof the porous ∞2{[Dy4O17]22–} sheets. The linkage of the ∞2{[Dy4O17]22–} partial structure to achieve a framework only takes place by [(Te4)O3]2– ions through Dy3–O–Te4–O–Dy4 bridges. Between the ∞2{[Dy4O17]22–} layers as well as within their channels there is still enough space left to accommodate the stereochemically active lone‐pair electrons at the Te4+ ions.