bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cisplatin-induced oxidative stress, apoptosis, and pro-inflammatory responses in chondrocytes through modulating LOX-1
Authors: Wu Chin-Hsien , Chou Wan-Ching , Jou I.-Ming , Tu Yuan-Kun , Ma Ching-Hou , Tsai Kun-Ling 
Published: February 2025

Abstract
Cisplatin is a potent and efficacious anticancer medication. In pediatric cancer, the height of the growth plate’s proliferating layer is known to be reduced by cisplatin, but researchers have not yet determined the specific mechanism behind this phenomenon. Lectin-like oxidized low-density lipoprotein receptor-1 is known to be involved in the development of osteoarthritis and atherosclerosis. The equilibrium of cartilage is regulated by LOX-1, but the function of LOX-1 in cisplatin-induced chondrocyte impairment remains unknown. Positive regulation of LOX-1 leads to increased cellular oxidative stress and cell damage. Research has shown that blocking of LOX-1 can reduce the chondrocyte damage and oxidative stress in cells induced by oxidized LDL treatment. However, the role of LOX-1 in cisplatin-mediated chondrocyte damage is still unclear. This study found that cisplatin increased ROS concentration and p38, ERK phosphorylation. Cisplatin activated NF-κB in chondrocytes. In addition, LOX-1 small interfering RNA transfection mitigated cisplatin-induced apoptosis in TC28a2 cells. Phosphorylated extracellular signal-regulated kinase and p38 were dose-dependently increased by administration of cisplatin. Silencing LOX-1 or MAPK inhibition reduces cisplatin-caused apoptosis. The findings suggest that cisplatin-induced growth plate dysfunction operates through the LOX-1/p38/NF-κB signaling pathway.
Exploring new frontiers in oncofertility preservation: a case of ovarian stimulation during pregnancy
Authors: Pirooznia Parisa , Meighani Esmat Mashhadi , Ghaffari Firouzeh 
Published: February 2025

Abstract

Background
The standard treatment for Pregnancy-Associated Breast Cancer (PABC) includes surgery and neoadjuvant chemotherapy, which can impair fertility, emphasizing the critical need for fertility preservation in these patients. This case report discusses a breast cancer patient who was found to be pregnant shortly after starting treatment. Despite the pregnancy and increased levels of βHCG and progesterone, the ovarian stimulation cycle yielded a satisfactory number of mature oocytes and high-quality embryos.

Case presentation
A 40-year-old woman, G1Ab1 (Gravida1Abortion1), who was diagnosed with Invasive Ductal Carcinoma with negative receptors (Estrogen Receptor, Progesterone Receptor, and Human Epidermal Growth Factor Receptor 2) was referred to the oncofertility unit of the Royan Infertility Center for fertility preservation prior to the commencement of chemotherapy. Following necessary consultations and procedures, and confirming a negative pregnancy test, a random start letrozole-based protocol was initiated for ovarian stimulation. During the cycle, a positive pregnancy test was encountered. Despite the positive test, the cycle continued, and on day 13 of the cycle, triggering was performed with a GnRH agonist. A puncture was performed 36 h later, yielding 12 oocytes and 8 embryos.

Conclusion
This case highlights the feasibility of adapting random-start ovarian stimulation protocols during pregnancy, warranting further investigation in similar clinical scenarios.
Cross-species imputation and comparison of single-cell transcriptomic profiles
Authors: Zhang Ran , Yang Mu , Schreiber Jacob , O’Day Diana R. , Turner James M. A. , Shendure Jay , Noble William Stafford , Disteche Christine M. , Deng Xinxian 
Published: February 2025

Abstract
Cross-species comparison and prediction of gene expression profiles are important to understand regulatory changes during evolution and to transfer knowledge learned from model organisms to humans. Single-cell RNA-seq (scRNA-seq) profiles enable us to capture gene expression profiles with respect to variations among individual cells; however, cross-species comparison of scRNA-seq profiles is challenging because of data sparsity, batch effects, and the lack of one-to-one cell matching across species. Moreover, single-cell profiles are challenging to obtain in certain biological contexts, limiting the scope of hypothesis generation. Here we developed Icebear, a neural network framework that decomposes single-cell measurements into factors representing cell identity, species, and batch factors. Icebear enables accurate prediction of single-cell gene expression profiles across species, thereby providing high-resolution cell type and disease profiles in under-characterized contexts. Icebear also facilitates direct cross-species comparison of single-cell expression profiles for conserved genes that are located on the X chromosome in eutherian mammals but on autosomes in chicken. This comparison, for the first time, revealed evolutionary and diverse adaptations of X-chromosome upregulation in mammals.
An influential study of a time-delayed epidemic model incorporating vaccination and treatment interventions
Authors: Pathak Sushil , Kota Venkata Ratnam 
Published: February 2025

Abstract
A mathematical model delineating the dynamics of infectious diseases under appropriate vaccination and treatment efforts is analyzed. The study thoroughly investigates the implications of time delays inherent in vaccine preparation and administration, the transition from treatment to recovery, and the timeline from exposure to infection. The model is strengthened by establishing essential mathematical properties such as nonnegativity, boundedness, existence of solutions, and equilibria. An estimate of the basic reproduction number is provided to facilitate a preliminary understanding of disease behavior. Furthermore, the local stability of disease-free and endemic equilibria is examined in relation to the basic reproduction number, highlighting the model’s capacity to predict disease dynamics. Moreover, the global stability of the equilibria is essential for understanding the ultimate impact of the disease, as the initial incidence may or may not continue its influence in the presence of counteracting effects such as vaccination and treatment throughout the system. Finally, the results are tested with parameters chosen from recently collected data on the dynamics of COVID-19, and numerical examples are presented to support the theoretical concepts proposed. Simulations visualize these numerical examples.
Is it about substituting an addiction with another? development and initial psychometric properties of the first heated tobacco products addiction questionnaire (HeaTPAQ)
Authors: Fekih-Romdhane Feten , Hallit Rabih , Malaeb Diana , Sakr Fouad , Dabbous Mariam , Obeid Sahar , Hallit Souheil 
Published: February 2025

Abstract

Background
Public health experts currently agree that heated tobacco products (HTPs) pose a significant health risk for their consumers. The same concentrations and speed of delivery of nicotine found for HTPs and conventional combustion cigarettes make it necessary to consider the addictiveness of HTPs, and provide precise diagnostic instruments to serve as the basis for effective treatment plans. Therefore, the main objectives of this study were to design a questionnaire for HTPs addiction called “Heated Tobacco Products Addiction Questionnaire (HeaTPAQ)” and to examine its psychometric properties.

Methods
Adults from the general population of Lebanon (n = 754) were administered the HeatPAQ, along with the Fagerström test for nicotine dependence (FTND), the Caffeine Use Disorder Questionnaire, the Generalized Anxiety Disorder 7-item, and the Patient Health Questionnaire-9. We split the main sample into two subsamples; subsample 1 consisting of 33% of the participants used for the exploratory factor analysis (EFA) (n = 246; mean age 27.82 ± 9.38 years) and subsample 2 consisting of 67% of the participants used for the confirmatory factor analysis (CFA) (n = 508; mean age 27.81 ± 8.80 years).

Results
EFA then CFA analyses revealed a one-factor model consisting of 13 items with acceptable fit to the data. The HeaTPAQ reached excellent internal consistency coefficients, with both Cronbach’s α and McDonald’s ω values of 0.96. The one-dimensional structure of the HeaTPAQ was found to be invariant across sex groups. Convergent validity was demonstrated through significant positive correlation with FTND scores. Furthermore, HeaTPAQ scores correlated positively with measures of caffeine addiction, anxiety and depression, which suggests the adequate concurrent validity of the scale.

Conclusion
Findings suggest that the HeatPAQ is a specific, short and simple-to-use self-report questionnaire to assess HTPs addiction reliably and validly. Pending future studies confirming our results, we hope that the HeatPAQ will facilitate routine screening for HTPs addiction, which is an essential step towards appropriate prevention and intervention efforts and to inform policy makers.
TET2 downregulation enhances the antitumor efficacy of CD19 CAR T cells in a preclinical model
Authors: Kim Yeongrin , Jeun Moonjung , Lee Heung Kyoung , Choi Ji U , Park Simon , Park Chi Hoon 
Published: February 2025

Abstract
Chimeric antigen receptor (CAR) T cell therapy has demonstrated significant clinical efficacy in patients with hematologic cancers. However, long-term follow-up studies indicate that only 50% of patients remain in complete remission after three years. To overcome these limitations, we investigated a strategy to enhance the antitumor activity of CAR T cells through gene modification. Based on previous research results demonstrating that CAR T cells with disrupted TET2, a methylcytosine dioxygenase, exhibit enhanced antitumor effects compared to conventional CAR T, we developed CAR T cells in which TET2 is downregulated by TET2 shRNA. Among the screened TET2-specific shRNAs, TET2-shRNA-1 was identified as the most effective sequence for gene silencing. Using this sequence, we constructed an all-in-one vector co-expressing CD19 CAR and TET2 shRNA. In vitro studies demonstrated that TET2 knockdown CD19 CAR T cells exhibited comparable cytolytic activity against CD19-positive cancer cells compared to conventional CD19 CAR T cells. However, interestingly, in xenograft mouse model using NSG mice, TET2 knockdown CAR T cells showed significantly improved antitumor activity compared to conventional CAR T cells. Our study demonstrates that shRNA-mediated knockdown of TET2 is a promising strategy to enhance the antitumor activity of CD19 CAR T cells in a preclinical model.
Suppression of CYLD by HER3 confers ovarian cancer platinum resistance via inhibiting apoptosis and by inducing drug efflux
Authors: Zhang Ye , Qiu Jian-Ge , Wang Wei , Sun Fan-Li , Wang Xue , Liu Wen-Jing , Jia Xiao-Yu , Ji Hongbin , Wang Lin , Jiang Bing-Hua 
Published: February 2025

Abstract

Background
Ovarian cancer (OC) is the most pathogenic gynecological malignant tumor in the world. Due to the difficulty of early diagnosis, most of patients developed chemo-resistance and recurrence during/after chemotherapy.

Methods
CCK8 and flow cytometry were utilized to assess drug sensitivity and apoptosis in parental and drug resistant cell lines. CYLD knockdown or overexpressed cells were employed to investigate its regulatory involvement in DDP resistance. Clinical tumor samples have been utilized to investigate the clinical relevance of CYLD. The drug synergistic effects were investigated through drug combination methods and a nude mice model with ABCB1 inhibitor or HER3 inhibitor.

Results
In this study, we found that CYLD levels were significantly reduced in DDP-resistant cancer tissues and cells compared to the normal tissues and cells. CYLD knockdown in DDP-sensitive cells was sufficient to converse the cells to become DDP resistant by reducing cell apoptosis through increasing Bcl-XL and inhibiting Bax, and by increasing drug efflux via upregulating ABCB1 expression. HER3 expression levels were substantially higher in resistant cancer tissues and cells, and HER3 was the upstream facilitator of suppressing CYLD expression via STAT3 signaling. Furthermore, overexpression of CYLD in resistant cells increased sensitivity to platinum-based chemotherapy both in vitro and in vivo. ABCB1 was a key downstream target of CYLD for regulating tumor growth and therapeutic resistance both in vitro and in vivo, CYLD knockdown promoted the translocation of p65 to nucleus which increased ABCB1 expression through transcriptional activation. High expression levels of HER3 rendered CYLD suppression, consequently, mediated DDP resistance by blocking cell apoptosis pathways and promoting the drug efflux in ovarian cancer.

Conclusions
Our findings identify novel HER3/CYLD/ABCB1 axis that regulate tumor growth and DDP resistance, which may be used as potential novel therapeutic target(s) to overcome ovarian cancer DDP resistance.
How do non-independent host movements affect spatio-temporal disease dynamics? Partitioning the contributions of spatial overlap and correlated movements to transmission risk
Authors: Vargas Soto Juan S. , Kosiewska Justin R. , Grove Dan , Metts Dailee , Muller Lisa I. , Wilber Mark Q. 
Published: February 2025

Abstract

Background
Despite decades of epidemiological theory making relatively simple assumptions about host movements, it is increasingly clear that non-random movements drastically affect disease transmission. To better predict transmission risk, theory is needed that quantifies the contributions of both fine-scale host space use and non-independent, correlated host movements to epidemiological dynamics.

Methods
We developed and applied new theory that quantifies relative contributions of fine-scale space use and non-independent host movements to spatio-temporal transmission risk. Our theory decomposes pairwise spatio-temporal transmission risk into two components: (i) spatial overlap of hosts—a classic metric of spatial transmission risk – and (ii) pairwise correlations in space use – a component of transmission risk that is almost universally ignored. Using analytical results, simulations, and empirical movement data, we ask: under what ecological and epidemiological conditions do non-independent movements substantially alter spatio-temporal transmission risk compared to spatial overlap?

Results
Using theory and simulation, we found that for directly transmitted pathogens even weak pairwise correlations in space use among hosts can increase contact and transmission risk by orders of magnitude compared to independent host movements. In contrast, non-independent movements had reduced importance for transmission risk for indirectly transmitted pathogens. Furthermore, we found that if the scale of pathogen transmission is smaller than the scale where host social decisions occur, host movements can be highly correlated but this correlation matters little for transmission. We applied our theory to GPS movement data from white-tailed deer (Odocoileus virginianus). Our approach predicted highly seasonally varying contributions of the spatial and social drivers of transmission risk – with social interactions augmenting transmission risk between hosts by greater than a factor of 10 in some cases, despite similar degrees of spatial overlap. Moreover, social interactions could lead to a distinct shift in the predicted locations of transmission hotspots, compared to joint space use.

Conclusions
Our theory provides clear expectations for when non-independent movements alter spatio-temporal transmission risk, showing that correlated movements can reshape epidemiological landscapes, creating transmission hotspots whose magnitude and location are not necessarily predictable from spatial overlap.
From pox to protection: understanding Monkeypox pathophysiology and immune resilience
Authors: Ganesan Alagammai , Arunagiri Thirumalai , Mani Suganandhini , Kumaran Vamsi Ravi , Kannaiah Kanaka Parvathi , Chanduluru Hemanth Kumar 
Published: February 2025

Abstract
The Monkeypox virus (MPXV), which causes Monkeypox (Mpox) is an invasive ailment with global implications. MPXV, categorized within the Orthopoxvirus genus, exhibits diverse clades with varying fatality rates. Initially discovered in monkeys and later in humans, the disease predominantly affects regions across West and Central Africa. Clinical manifestations encompass a spectrum from mild flu-like symptoms to severe eruptions. This article aims to give the scientific community a comprehensive overview of Mpox Pathophysiology and delve into the intricate landscape of host immune responses against MPXV infection. It offers crucial insights into the virus's Pathophysiology, spanning its entry, replication, dissemination, and elicited host responses. The immune reaction to Mpox involves innate immunity, B-cell immunity, and T-cell immunity. Moreover, this review underscores the immunological response and resistance mechanisms against MPXV. It also highlights imperative research areas warranting prioritization to devise more efficacious treatments for controlling viral propagation within healthcare systems. In addition, it gives us a look into possible futures that could help the progress of immunotherapies and cutting-edge biotechnological solutions for protecting against MPXV.
Health system reform in the context of COVID-19: a policy brief outlining lessons from Ireland’s journey towards the goal of universal healthcare
Authors: Parker Sarah , Schulmann Katharine , Bruen Carlos , Burke Sara 
Published: February 2025

Abstract
The COVID-19 pandemic has presented unique challenges and opportunities for health system reform globally. In Ireland, this period coincided with the early stages of the Sláintecare reform plan, a core goal of which is to establish universal healthcare. This policy brief synthesises key research findings from 13 studies carried out under the Foundations research programme to harness key learnings from the pandemic response for health system change. The analysis reveals how the COVID-19 crisis accelerated health system reforms in Ireland, breaking from a history of incremental change to implement rapid innovations towards universal healthcare. While a ‘new normal’ has emerged, the challenge remains to integrate these rapid developments into enduring health system improvements under evolving governance and leadership in the COVID-19 context. Three significant implications for health systems research and policy are identified: 1) Political consensus is essential for sustained health system reform, particularly during crises; 2) Adaptive health systems that can transform challenges into reform opportunities are crucial; and 3) Co-production in research enhances policy acceptability and implementation by aligning it with real-world complexities. Leveraging these pandemic-driven insights will be key to ensuring that the swift adaptations and lessons learned will transition into lasting elements of Ireland’s health system.
Advanced spectroscopic techniques for body fluid identification: complementing conventional methods
Authors: Joon Vandana , Dhankar Vijay , Chauhan Tanya , Verma Satish Kumar 
Published: February 2025

Abstract

Background
Body fluids (BFs) are highly important in forensically relevant scenarios. Historically, conventional techniques have been used for their identification and detection purposes. However, there is no conventional technology available that can detect a mixture of body fluids in one go.

Main body
There is a need for an advanced confirmatory technique that can reliably detect all types of body fluids even in trace forms, whether in pure form or in mixture form. The discussed spectroscopic techniques include raman spectroscopy, fourier transform infrared (FTIR), mass spectroscopy (MS), and nuclear magnetic resonance spectroscopy (NMR).

Conclusions
These reviewed techniques have proven to be advanced, confirmatory, mostly non-destructive, sensitive, reliable, and reproducible techniques in body fluid identification when combined with advanced statistical analysis and available reference databases. 1H NMR spectroscopy can be an emerging versatile technique with wide-ranging forensic applications. The progressive research related to these advanced techniques can revolutionize the field of forensics.
Assessment of eucalyptus oil integrated with carboxymethyl cellulose (CMC) for controlling postharvest soft rot in Citrus sinensis fruits induced by Colletotrichum gloeosporioides and Aspergillus niger
Authors: Adeogun Oluwagbenga Oluwasola , Oluwa Omoteso Karfeel , Nejo Ayorinde Olufunke , Salami Simbiat Oduneye , Egwu Patience Chileenwa , Adekunle Adedotun Adeyinka 
Published: February 2025

Abstract

Background
The demand for oranges remains unsatisfied due to low market yields, often attributed to postharvest diseases. Addressing this issue necessitates the exploration of natural antimicrobials as alternatives to synthetic preservatives to ensure safety and extend shelf life.

Methodology
This study investigated the efficacy of carboxymethyl cellulose integrated with eucalyptus oil in mitigating postharvest soft rot in Citrus sinensis fruits induced by Colletotrichum gloeosporioides and Aspergillus niger. Gas chromatography/mass spectrometry (GC/MS) was employed to identify eucalyptus oil phytoconstituents. In-vitro and in-vivo assays were conducted to assess the coating’s antifungal properties and quality parameters (weight loss, antioxidant activities, pH, total soluble solids, water activity, firmness, and electrical conductivity) over 20 days. The impacts of the coating on the catalase and peroxidase activities in orange fruits were also evaluated.

Results
GC/MS analysis revealed eucalyptol (76.60%) as the predominant constituent in eucalyptus oil. The study confirmed C. gloeosporioides and A. niger as causative agents of soft rot in orange fruits. Integration of carboxymethyl cellulose with eucalyptus oil positively influenced orange fruit quality parameters, including weight loss, antioxidant activities, pH, total soluble solids, water activity, firmness, and electrical conductivity, while enhancing antioxidant enzymatic activities.

Conclusions
This study demonstrates the efficacy of the carboxymethyl cellulose-eucalyptus oil coating as a potentially cost-effective approach to improving the safety, control of postharvest diseases, and quality of orange fruits.