bluuebunny/crossref_metadata_2025_split huggingface dataset:
A case of methotrexate embryopathy with holoprosencephaly, expanding the phenotype
Authors: Seidahmed Mohammed Zain , Shaheed Meeralebbae M. , Abdulbasit Omar B. , Al Dohami Hessa , Babiker Mirghani , Abdullah Mohammed A. , Abomelha Abdullah M. 
Published: February 2006

AbstractBACKGROUNDMethotrexate (MTX) embryopathy was described nearly 50 years ago, when this agent began to be used as a cancer treatment and abortifacient. In this report we describe a case with typical features of MTX syndrome together with new features to expand the phenotype.CASEA 29‐year‐old woman decided to terminate her unwanted pregnancy because of ill health, as she had conceived soon after her last delivery by cesarian section. At 6 weeks of gestation, she took 2.5 mg of MTX 3 times a day for 7 days. The pregnancy termination failed, and the pregnancy was carried to term. A female infant was delivered who was growth retarded and had characteristic features of MTX embryopathy in addition to holoprosencephaly and other brain malformations, facial hypertrichosis, and long eyelashes—features that have not hitherto been described.CONCLUSIONSWe report the first case of holoprosencephaly in association with MTX exposure during the first 6 weeks of gestation. Physicians and the public should be aware of the effects of MTX on the fetus during pregnancy. Birth Defects Research (Part A), 2006. © 2006 Wiley‐Liss, Inc.
The trans–cis isomerization reaction dynamics in sensory rhodopsin II by femtosecond time‐resolved midinfrared spectroscopy: Chromophore and protein dynamics
Authors: Diller Rolf , Jakober Ruth , Schumann Christian , Peters Frank , Klare Johann P. , Engelhard Martin 
Published: February 2006

AbstractTransient infrared (IR) vibrational spectroscopy at subpicosecond time resolution on sensory rhodopsin II from Natronomonas pharaonis, NpSRII, has been performed for the first time. The experiments yield three time constants for the description of the primary photoinduced reaction dynamics, i.e. 0.5, 3.7–4.4, and 11 ps. The data are consistent with a sequential reaction scheme, with the isomerization taking place within 0.5 ps, succeeded by an electronic ground state relaxation. The 11 ps component, observed at 1550 and 1530 cm−1, is attributed to dynamics of protein vibrational bands, possibly amide II bands of the protein backbone, perturbed by the ultrafast retinal photoisomerization. Similar observations, yet not as strongly expressed, have been made earlier in bacteriorhodopsin and halorhodopsin. © 2006 Wiley Periodicals, Inc. Biopolymers 82: 358–362, 2006This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Potential of the marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystems
Authors: Fu Wantao , Sun Liming , Zhang Xichang , Zhang Wei 
Published: February 2006

AbstractThe aim of this article is to investigate the potential of using sponges as a bioremediator to remove pathogenic bacteria in integrated aquaculture ecosystems. Using the inter‐tidal marine sponge Hymeniacidon perleve as a model system, the ability of removing the most common pathogens Escherichia coli and Vibrio anguillarum II in aquaculture waters was screened in laboratory tests. In sterilized natural seawater (SNSW) supplemented with E. coli at (7.0–8.3) × 106 cells/mL, H. perleve can remove an average 96% of E.coli within 10.5 h at a filter rate of ca. (7.53–8.03) × 107 cells/h · g of fresh sponge in two independent tests. Despite the removal efficiency and filter rate are similar; the clearance rates (CR) vary significantly among individual sponge specimens and between two batches. For the tests on V. anguillarum II in SNSW, about 1.5 g fresh sponges can keep the pathogen growth under control at a lower initial density 3.6 × 104 cells/mL of 200 mL water volume. Further tests were done for 24 h using about 12 g fresh sponge in 2‐L actual seawater collected from two aquaculture sites that have ca. eightfold difference in pathogenic bacteria load. The concentrations of E. coli, Vibrio, and total bacteria at 24 h in treatment groups were markedly lower, at about 0.9%, 6.2%–34.5%, and 13.7%–22.5%, respectively, of those in the control. Using a fluoresce stain 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate, E. coli, and V. anguillarum II cells were stained and fed to sponges in two independent tests. The confocal microscope observation confirmed that the sponges filtering‐retained and digested these bacteria by phagocytosis. © 2006 Wiley Periodicals, Inc.
Tolerance and adaptation of ethanologenic yeasts to lignocellulosic inhibitory compounds
Authors: Keating Jeffrey D. , Panganiban Chris , Mansfield Shawn D. 
Published: February 2006

AbstractSynthetic mixtures of predominant lignocellulosic hexose sugars were supplemented with separate aliquots of three inhibitory compounds (furfural, hydroxymethylfurfural (HMF), and acetic acid) in a series of concentrations and fermented by the spent sulfite liquor (SSL)‐adapted yeast strain Tembec T1 and the natural isolate Saccharomyces cerevisiae (S. cerevisiae) Y‐1528 to compare tolerance and assess fermentative efficacy. The performance of Y‐1528 exceeded that of Tembec T1 by a significant margin, with faster hexose sugar consumption, higher ethanol productivity, and in the case of furfural and HMF, faster inhibitor consumption. Nevertheless, furfural had a dose‐proportionate effect on sugar consumption rate and ethanol productivity in both strains, but did not substantially affect ethanol yield. HMF had a similar effect on sugar consumption rate and ethanol productivity, and also lowered ethanol yield. Surprisingly, acetic acid had the least impact on sugar consumption rate and ethanol productivity, and stimulated ethanol yield at moderate concentrations. Sequential iterations of softwood (SW) and hardwood (HW) SSL were subsequently inoculated with the two yeast strains in order to compare adaptation to, and performance in lignocellulosic substrates in a cell recycle batch fermentation (CRBF) regime. Both strains were severely affected by the HW SSL, which was attributed to specific syringyl lignin‐derived degradation products and synergistic interactions between inhibitors. Though ethanologenic capacity was preserved, a net loss of performance was evident from both strains, indicating the absence of adaptation to the substrates, regardless of the sequence in which the SSL types were employed. © 2006 Wiley Periodicals, Inc.
A motion capture‐based control‐space approach for walking mannequins
Authors: Pettre Julien , Laumond Jean‐Paul 
Published: February 2006

AbstractVirtual mannequins need to navigate in order to interact with their environment. Their autonomy to accomplish navigation tasks is ensured by locomotion controllers. Control inputs can be user‐defined or automatically computed to achieve high‐level operations (e.g. obstacle avoidance). This paper presents a locomotion controller based on a motion capture edition technique. Controller inputs are the instantaneous linear and angular velocities of the walk. Our solution works in real time and supports at any time continuous changes of inputs. The controller combines three main components to synthesize locomotion animations in a four‐stage process. First, the Motion Library stores motion capture samples. Motion captures are analysed to compute quantitative characteristics. Second, these characteristics are represented in a linear control space. This geometric representation is appropriate for selecting and weighting three motion samples with respect to the input state. Third, locomotion cycles are synthesized by blending the selected motion samples. Blending is done in the frequency domain. Lastly, successive postures are extracted from the synthesized cycles in order to complete the animation of the moving mannequin. The method is demonstrated in this paper in a locomotion‐planning context. Copyright © 2006 John Wiley & Sons, Ltd.
Intracoronary stent dislodgment: Updated strategy enabled by the new generation of materials
Authors: Patterson Mark , Slagboom Ton 
Published: February 2006

AbstractStent dislodgment remains a vexing problem in percutaneous coronary intervention. The technological improvements in machine‐crimped premounted stents have led to a reduced incidence of dislodgment but may have exacerbated the problem of stent dislodgment (SD). Previous reports describing the management of SD have concentrated predominantly on manually mounted stents. We present a case where the position of a dislodged premounted stent in the distal left main stem was unfavorable for previously described solutions. The problem was elegantly resolved by taking advantage of the improved performance of the current generation of wires and balloons to achieve a rapid first‐rate clinical outcome. © 2006 Wiley‐Liss, Inc.
Nonrecurring transient cortical blindness after coronary angiography: A role for hypoventilation and hypercarbia?
Authors: Danenberg Haim D. , Lotan Chaim 
Published: February 2006

AbstractA 73‐year‐old morbidly obese patient suffered cortical blindness following coronary angiography. Symptoms rapidly resolved and did not recur following a repeated procedure. Hypoventilation due to narcotics with subsequent hypercarbia and blood‐brain barrier breaching may be implicated in the pathogenesis of this complication. © 2006 Wiley‐Liss, Inc.
Peptide Recognition: Encapsulation and α‐Helical Folding of a Nine‐Residue Peptide within a Hydrophobic Dimeric Capsule of a Bowl‐Shaped Host
Authors: Tashiro Shohei , Tominaga Masahide , Yamaguchi Yoshiki , Kato Koichi , Fujita Makoto 
Published: February 2006

AbstractA dimeric capsule of coordination bowl 1 encapsulated a nine‐residue peptide (Trp‐Ala‐Glu‐Ala‐Ala‐Ala‐Glu‐Ala‐Trp; 2) within the large hydrophobic cavity in water, and stabilized the α‐helical conformation of bound 2. An NMR titration experiment revealed that monomeric bowl 1 recognized two Trp residues at the both terminals of 2 through 1/2=1:1 to 2:1 complexation. The 1:1 and 2:1 species exist in equilibrium even in the presence of excess 1. It was found that the formation of the 2:1 complex, in which two bowls of 1 wrapped the whole of 2, became dominant by the addition of NaNO3 due to the fact that the enhanced ion strength increased the hydrophobic interaction between Trp residues and the cavity of 1. The α‐helical conformation of 2 within the dimeric capsule of 1 was elucidated from detailed NOESY analysis.
A Photoactivated trans‐Diammine Platinum Complex as Cytotoxic as Cisplatin
Authors: Mackay Fiona S. , Woods Julie A. , Moseley Harry , Ferguson James , Dawson Alice , Parsons Simon , Sadler Peter J. 
Published: February 2006

AbstractThe synthesis and X‐ray structure (as the tetrahydrate) of the platinum(IV) complex trans,trans,trans‐[Pt(N3)2(OH)2(NH3)2] 3 are described and its photochemistry and photobiology are compared with those of the cis isomer cis,trans,cis‐[Pt(N3)2(OH)2(NH3)2] 4. Complexes 4 and 3 are potential precursors of the anticancer drug cisplatin and its inactive trans isomer transplatin, respectively. The trans complex 3 is octahedral, contains almost linear azide ligands, and adopts a layer structure with extensive intermolecular hydrogen bonding. The intense azide‐to‐platinum(IV) charge‐transfer band of complex 3 (285 nm; ε=19 500 M−1 cm−1) is more intense and bathochromically shifted relative to that of the cis isomer 4. In contrast to transplatin, complex 3 rapidly formed a platinum(II) bis(5′‐guanosine monophosphate) (5′‐GMP) adduct when irradiated with UVA light, and did not react in the dark. Complexes 3 and 4 were non‐toxic to human skin cells (keratinocytes) in the dark, but were as cytotoxic as cisplatin on irradiation for a short time (50 min). Damage to the DNA of these cells was detected by using the “comet” assay. Both trans‐ and cis‐diammine platinum(IV) diazide complexes therefore have potential as photochemotherapeutic agents.
Characterization of bone marrow T cells in monoclonal gammopathy of undetermined significance, multiple myeloma, and plasma cell leukemia demonstrates increased infiltration by cytotoxic/Th1 T cells demonstrating a squed TCR‐Vβ repertoire
Authors: Pérez‐Andres Martin , Almeida Julia , Martin‐Ayuso Marta , Moro Maria Jesus , Martin‐Nuñez Guillermo , Galende Josefina , Hernandez Jose , Mateo Gema , San Miguel Jesus F. , Orfao Alberto 
Published: February 2006

AbstractBACKGROUNDThe majority of studies published to date regarding the role of the bone marrow (BM) microenvironment in the pathogenesis of monoclonal gammopathies (MG) have focused on the interaction between stroma cells and plasma cells, whereas information concerning the lymphocytes infiltrating the tumor microenvironment is scanty.METHODSThe authors measured the distribution, TCR‐Vβ repertoire, immunophenotype, and functional characteristics of different subsets of BM T lymphocytes from 61 nontreated patients with MG (30 patients with MG of undetermined significance [MGUS], 27 patients with multiple myeloma [MM], and 4 patients with plasma cell leukemia [PCL]).RESULTSThe authors found a significantly increased rate of BM infiltration by T cells in all patient groups, at the expense of CD4+CD8− and CD4−CD8− T lymphocytes and both CD4+CD28− and CD8+CD28− cytotoxic/effector T cell subsets, and associated with TCR‐Vβ expansions in both CD4+ and CD8+ BM T cells in the majority of patients with MGUS, MM, and PCL. Moreover, the percentage of T cells secreting interferon (IFN)‐γ was found to be increased (P ≤ 0.05) both in CD4+ and CD8+ T cells in MGUS and MM patients, and a higher plasma concentration of IFN‐γ was found in patients with MM. It is interesting to note that a positive correlation was noted between the proportion of CD28− and both the percentage of IFN‐γ–secreting cells and the proportion of expanded TCR‐Vβ lymphocytes within the total BM CD4+ T cells.CONCLUSIONSThe results of the current study demonstrated an increased infiltration of BM by T cells associated with frequent TCR‐Vβ expansions and a more prominent cytotoxic/Th1 phenotype in all the patient groups studied. Cancer 2006. © 2006 American Cancer Society.
Survival analysis after resection of metastatic disease followed by peptide vaccines in patients with Stage IV melanoma
Authors: Tagawa Scott T. , Cheung Eric , Banta Warren , Gee Conway , Weber Jeffrey S. 
Published: February 2006

AbstractBACKGROUNDMetastatic melanoma carries a poor prognosis, with a median survival of 7–9 months. Surgical resection of metastatic disease has been advocated to improve survival. Immunotherapy after metastasectomy may further improve the outcome for high‐risk resected disease.METHODSCharts from patients treated on institutional vaccine trials were analyzed. Patients with American Joint Committee on Cancer (AJCC) Stage IV melanoma who underwent surgical resection of metastatic sites followed by treatment on a peptide vaccine trial were eligible for this study. Survival was calculated from the date of enrollment on the clinical trial.RESULTSForty‐one patients met inclusion criteria. The median age was 56.5 years, with approximately equal numbers of men and women. The ECOG performance status was 0 in all patients. Approximately 46% of patients underwent resection of visceral metastases before vaccine. The median follow‐up was 5.6 years. The median overall survival was 3.8 years.CONCLUSIONSIn selected patients with AJCC Stage IV melanoma, resection of metastatic disease followed by vaccine therapy can result in long‐term survival. Cancer 2006. © 2006 American Cancer Society.
Clinical and radiologic predictors of invasive pulmonary aspergillosis in cancer patients
Authors: Hachem Ray , Sumoza David , Hanna Hend , Girgawy Essam , Munsell Mark , Raad Issam 
Published: February 2006

AbstractBACKGROUNDInvasive pulmonary aspergillosis (IPA) is a life‐threatening infection in immunocompromised patients. The outcome of such infections depends on early diagnosis and prompt initiation of therapy. The objective of the current study was to determine the significant predictive factors that characterize IPA in patients with cancer.METHODSThe clinical characteristics and computed tomography (CT) findings for 47 cases with IPA were retrospectively reviewed and compared with 49 controls (patients diagnosed on autopsy with nonfungal pneumonias). Data from all 96 patients were modeled using multivariate logistic regression. Subgroups of patients with common characteristics and outcomes were identified.RESULTSLeukemia, neutropenia, cavitation, and nodular lesions occurred significantly more often among cases than controls (P = 0.04, 0.004, 0.04, and 0.02, respectively). A quantitative scoring system was developed that could be used to identify patients as being at low, medium, and high risk for IPA.CONCLUSIONSIPA should be highly suspected in leukemia patients with profound neutropenia, pleuritic chest pain, and cavitary or nodular lesions detected on CT scan. These predictive factors can be used to indicate when early prophylactic and therapeutic antifungal interventions should be initiated. Cancer 2006. © 2006 American Cancer Society.
Electrophoresis of DNA in human genetic diagnostics – state‐of‐the‐art, alternatives and future prospects
Authors: Gödde René , Akkad Denis‐Amer , Arning Larissa , Dekomien Gabriele , Herchenbach Julia , Kunstmann Erdmute , Meins Moritz , Wieczorek Stefan , Epplen Jörg T. , Hoffjan Sabine 
Published: February 2006

AbstractElectrophoretic separation of nucleic acids according to their molecular weights has dominated the methods' spectrum in molecular genetics for nearly half a century. We review the current methodological basis and evaluate its impact with special reference to new developments in the microarray technology. Although electrophoresis may be made redundant for many applications in DNA diagnostics within a few years, a number of electrophoretic vestiges will remain irreplaceable in the foreseeable future.
Parallel analysis of biomolecules on a microfabricated capillary array chip
Authors: Shen Zheng , Liu Xiaojun , Long Zhicheng , Liu Dayu , Ye Nannan , Qin Jianhua , Dai Zhongpeng , Lin Bingcheng 
Published: February 2006

AbstractThis paper focused on a self‐developed microfluidic array system with microfabricated capillary array electrophoresis (μ‐CAE) chip for parallel chip electrophoresis of biomolecules. The microfluidic array layout consists of two common reservoirs coupled to four separation channels connected to sample injection channel on the soda‐lime glass substrate. The excitation scheme for distributing a 20 mW laser beam to separation channels in an array is achieved. Under the control of program, the sample injection and separation in multichannel can be achieved through six high‐voltage modules' output. A CCD camera was used to monitor electrophoretic separations simultaneously in four channels with LIF detection, and the electropherograms can be plotted directly without reconstruction by additional software. Parallel multichannel electrophoresis of series biomolecules including amino acids, proteins, and nucleic acids was performed on this system and the results showed fine reproducibility.
A novel specific heparin‐binding activity of bovine folate‐binding protein characterized by capillary electrophoresis
Authors: Heegaard Niels H. H. , Hansen Steen I. , Holm Jan 
Published: February 2006

AbstractFolate‐binding proteins (FBPs) are ubiquitous, soluble and membrane‐bound high‐affinity receptors for folate, an essential nutrient involved in nucleic and amino acid metabolism. In the course of optimizing CE separation conditions for FBP purified from cow's milk we discovered a novel specific heparin‐binding activity of FBP by affinity CE. Heparin is a highly sulfated glycosaminoglycan and thus prone to induce anodic migration shifts of complexing analytes. Prior complexation of FBP with folate abolished heparin binding, and thus folate competes with heparin for binding to FBP. It was estimated that heparin bound several orders of magnitude less strongly than folate with an average dissociation constant in the 1–10 μM range. In contrast to the mobility shifts induced by heparin, free and folate‐bound FBP were not separated by CE. However, binding of folate induced a distinct increase in FBP‐peak symmetry, and using heparin as an affinity displacer, the free FBP in equilibrium with folate–FBP complexes could readily be separated from the complexes. While the folate–FBP interaction was too strong to be characterized quantitatively because of inadequate detection limits of a UV‐based detection system, it was possible to estimate the folate–FBP binding stoichiometry using this approach. The heparin interaction fractionated FBP into distinct subfractions, and the CE approach thus promises to be useful for unraveling the complex oligomerization behavior of FBP isoforms as well as for evaluating the FBP affinity for various species and analogs of glycosaminoglycans and folate.
Affinity monolithic capillary columns for glycomics/proteomics: 1. Polymethacrylate monoliths with immobilized lectins for glycoprotein separation by affinity capillary electrochromatography and affinity nano‐liquid chromatography in either a single column or columns coupled in series
Authors: Okanda Fred M. , El Rassi Ziad 
Published: February 2006

AbstractIn this report, microcolumn separation schemes involving monolithic capillary columns with immobilized lectins, and relevant to nanoglycomics/nanoproteomics were introduced. Positive and neutral monoliths based on poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) were designed for achieving lectin affinity chromatography (LAC) by nano‐LC and CEC. The positive monoliths (i.e., monoliths with cationic sites) afforded relatively high permeability in nano‐LC but lack predictable EOF magnitude and direction, while neutral monoliths provided a good compromise between reasonable permeability in nano‐LC and predictable EOF in CEC. Lectin affinity nano‐LC permitted the enrichment of classes of different glycoproteins having similar N‐glycans recognized by the immobilized lectin, whereas lectin affinity CEC provided the simultaneous capturing and separation of different glycoproteins due to differences in charge‐to‐mass ratio. Also, this investigation demonstrated for the first time the coupling of lectin capillary columns in series (i.e., tandem columns) for enhanced separation of glycoproteins by LAC using the CEC modality. Furthermore, in the coupled columns format, glycoforms of a given glycoprotein were readily separated.