bluuebunny/crossref_metadata_2025_split huggingface dataset:
An improved gel‐based DNA microarray method for detecting single nucleotide mismatch
Authors: Xiao Peng Feng , Cheng Lu , Wan Yuan , Sun Bei Li , Chen Zao Zao , Zhang Sheng You , Zhang Chung Ziu , Zhou Guo Hua , Lu Zu Hong 
Published: September 2006

Abstract3‐D polyacrylamide gel‐based DNA microarray platforms provide a high capacity for nucleic acids immobilization and a solution‐mimicking environment for hybridization. However, several technological bottlenecks still remain in these platforms, such as difficult microarray preparation and high fluorescent background, which limit their application. In this study, two new approaches have been developed to improve the convenience in microarray preparation and to reduce the background after hybridization. To control the polymerization process, solutions containing acrylamide‐modified oligonucleotide, acrylamide, glycerol and ammonium persulfate are spotted onto a functionalized glass slide, and then the slide is transferred to a vacuum chamber with TEMED, so that TEMED is vaporized and diffused into the spots to induce polymerization. By applying an electric field across a hybridized microarray to remove the nonspecifically bound labeled targets, this approach can solve the problem of high fluorescent background of the gel‐based microarray after hybridization. Experimental results show that our immobilization method can be used to construct high quality microarrays and exhibits good reproducibility. Moreover, the polymerization is not affected by PCR medium, so that PCR products can be used for microarray construction without being treated by commercial purification cartridges. Electrophoresis can improve the signal‐to‐noise significantly and has the ability to differentiate single nucleotide variation between two homozygotes and a heterozygote. Our results demonstrated that this is a reliable novel method for high‐throughput mutation analysis and disease diagnosis.
QSNPlite, a software system for quantitative analysis of SNPs based on capillary array SSCP analysis
Authors: Tahira Tomoko , Okazaki Yuko , Miura Kenichi , Yoshinaga Aki , Masumoto Kazumi , Higasa Koichiro , Kukita Yoji , Hayashi Kenshi 
Published: September 2006

AbstractWe present a newly developed software called “QSNPlite” that comprehensively interprets the data of SSCP and sequencing analyses obtained from capillary array electrophoresis systems used in the quantitative characterization of SNPs. QSNPlite assists in the genotyping of individuals with SNPs and in estimating the allele frequencies of SNPs using pooled DNA. We show that this estimation is accurate (mean absolute error, 1.4%) by comparing the results of the pooled analysis using QSNPlite with the true frequencies based on the allele counting after performing individual genotypings. The QSNPlite program runs on Windows XP and can be used to determine the allele frequencies of SNPs among a large number of individuals, such as in association studies of disease‐responsible genes using the candidate gene approach.
Where is all the pressure coming from? Messages from mothers and teachers about preschool children's appearance, diet and exercise
Authors: McCabe Marita P. , Ricciardelli Lina A. , Stanford Jacqueline , Holt Kate , Keegan Salley , Miller Louise 
Published: September 2006

AbstractThis study used interviews and qualitative analyses to investigate the nature of the messages that preschool children receive from mothers and teachers about their bodies, general appearance, exercise and eating practices. Participants were 10 female teachers and 53 mothers. The behaviours of the 53 children (24 boys, 29 girls) were also observed to determine the nature of their eating and exercise behaviours. The results demonstrated that both mothers and teachers expressed concerns about their own bodies. Mothers also communicated messages to their daughters about losing weight and messages to their sons about increasing their muscles. Both girls and boys were concerned about their appearance, particularly their clothes and hair. Girls also demonstrated some concerns about losing weight, and boys with increasing muscles. Implications of these results are discussed in terms of designing education programs for mothers, teachers and children to prevent the development of body image concerns and disordered eating among children. Copyright © 2006 John Wiley & Sons, Ltd and Eating Disorders Association.
Eating attitudes and their correlates among Canadian women concerned about their weight
Authors: Park Jungwee , Beaudet Marie P. 
Published: September 2006

AbstractObjectiveThis article examines the components of the Eating Attitudes Test‐26 (EAT‐26) and their association with selected demographic, psychosocial and health measures among women aged 15 years or older identified as concerned about their weight and who were part of a representative sample of the Canadian population in 2002.MethodAn exploratory factor analysis was used to identify the factor structure of the EAT‐26 and logistic regression models were constructed to examine the correlates of its first four dimensions.ResultsUnderweight women were more likely to report food and body image preoccupation and external pressure to eat while overweight women were less likely to do so. Being young was not associated with poor eating attitudes but self‐esteem was negatively related to them. Self‐esteem was, however, positively associated with self‐imposed dieting.DiscussionThe promotion of self‐esteem may reduce the prevalence of poor eating attitudes and prevent their immediate and long‐term negative consequences. © Crown Copyright 2006. Reproduced with the permission of Her Majesty's Stationery Office. Published by John Wiley & Sons, Ltd.
Controlling the orientation of bone osteopontin via its specific binding with collagen I to modulate osteoblast adhesion
Authors: Liu Lingyun , Qin Chunlin , Butler William T. , Ratner Buddy D. , Jiang Shaoyi 
Published: September 2006

AbstractOsteopontin (OPN) is an important matricellular protein that modulates cell functions. It is potentially an excellent surface‐coating component for engineered biomaterials. It is believed that in its preferred orientation and conformation on a surface, the functional domains of OPN such as the arginine–glycine–aspartic acid (RGD) motif will be presented to cells to the greatest extent. Previously, the authors demonstrated that OPN orientation could be modulated by surface charge. In this work, the authors attempt to control the orientation/conformation of bone OPN via its specific interactions with type I collagen. Surface plasmon resonance was used to confirm the specific binding between bone OPN and collagen I. A radiolabeled OPN adsorption assay was used to determine the amount of adsorbed OPN on tissue culture polystyrene (TCPS) surfaces with or without collagen I as an interlayer. An in vitro cell adhesion assay using osteoblast MC3T3‐E1 was performed to compare the functionality of collagen‐bound OPN and adsorbed OPN on TCPS. With the same amount of OPN on the surfaces, the number of cells adhered to collagen‐bound OPN is significantly higher than to OPN alone on TCPS. A cell inhibition assay using soluble GRGDSP peptides showed that a higher GRGDSP concentration was needed to completely block osteoblast adhesion to collagen‐bound OPN than to OPN directly on TCPS. Enhanced cell adhesion and higher blocking peptide concentration suggest that collagen‐bound bone OPN has a preferable orientation/conformation for cell adhesion compared with OPN alone on TCPS. Thus, the specific binding of OPN to collagen I may naturally orient OPN, thus influencing osteoblast adhesion. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Peri‐implant bone formation and implant integration strength of peptide‐modified p(AAM‐co‐EG/AAC) interpenetrating polymer network‐coated titanium implants
Authors: Barber Thomas A. , Ho James E. , De Ranieri Aladino , Virdi Amarjit S. , Sumner Dale R. , Healy Kevin E. 
Published: September 2006

AbstractInterpenetrating polymer networks (IPNs) of poly (acrylamide‐co‐ethylene glycol/acrylic acid) functionalized with an ‐Arg‐Gly‐Asp‐ (RGD) containing 15 amino acid peptides, derived from rat bone sialoprotein (bsp‐RGD(15), were grafted to titanium implants in an effort to modulate bone formation in the peri‐implant region in the rat femoral ablation model. Bone–implant contact (BIC) and bone formation within the medullary canal were determined using microcomputed tomography at 2 and 4 weeks postimplantation. BIC for bsp‐RGD(15)‐IPN implants was enhanced relative to hydroxyapatite tricalcium phosphate (HA‐TCP) coated implants, but was similar to all other groups. Aggregate bone formation neither indicated a dose‐dependent effect of bsp‐RGD(15) nor a meaningful trend. Mechanical testing of implant fixation revealed that only the HA‐TCP coated implants supported significant (>1 MPa) interfacial shear strength, despite exhibiting lower overall BIC, an indication that bone ingrowth into the rougher coating was the primary mode of implant fixation. While no evidence was found to support the hypothesis that bsp‐RGD(15)‐modified IPN coated implants significantly impacted bone–implant bonding, these results point to the lack of correlation between in vitro studies employing primary osteoblasts and in vivo wound healing in the peri‐implant region. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Genetic network and pathway analysis of differentially expressed proteins during critical cellular events in fracture repair
Authors: Li Xinmin , Wang Hali , Touma Edward , Rousseau Emma , Quigg Richard J. , Ryaby James T. 
Published: September 2006

AbstractBone repair consists of inflammation, intramembranous ossification, chondrogenesis, endochondral ossification, and remodeling. To better understand the translational regulation of these distinct but interrelated cellular events, we used the second generation of BD Clontech™ Antibody Microarray to dissect and functionally characterize proteins differentially expressed between intact and fractured rat femur at each of these cellular events. Genetic network analysis showed that proteins differentially expressed within a given cellular event tend to be physically or functionally correlated. Seventeen such interacting networks were established over five cellular events that were most frequently associated with cell cycle, cell death, cell‐to‐cell signaling and interaction, and cell growth and proliferation. Eighteen molecular pathways were significantly enriched during the bone repair process, of which ERK/MAPK, NF‐kB, PDGF, and T‐cell receptor signaling pathways were significant during three or more cellular events. The analyses revealed dynamic temporal expression patterns and cellular‐event‐specific functions. The inflammation event on Day 1 was characteristic of the cell cycle‐related molecular changes. The relative quiet stage of intramembranous ossification on Day 4 and the molecularly most active stage of chondrogenesis on Day 7 were featured by coordinated cell death and cell‐proliferation signals. Endochondral ossification on Day 14 experienced a clear transition from the molecular/cellular function to the physiological system development/function. The osteoclast‐mediated remodeling on Day 28 was highlighted by the integrin signaling pathway. The distinct changes in protein expression during these cellular events provide a molecular basis for developing cellular event‐targeted therapeutic strategy to accelerate bone healing. J. Cell. Biochem. 100: 527–543, 2007. © 2006 Wiley‐Liss, Inc.
Long‐term effects of tumor necrosis factor‐α treatment on insulin signaling pathway in HepG2 cells and HepG2 cells overexpressing constitutively active Akt/PKB
Authors: Gupta Dhananjay , Varma Shailly , Khandelwal Ramji L. 
Published: September 2006

AbstractTumor necrosis factor‐α (TNF‐α) mediated attenuation of insulin signaling pathway is an important cause in several disorders like obesity, obesity linked diabetes mellitus. TNF‐α actions vary depending upon concentration and time of exposure in various cells. In the present study, the effects of long‐term TNF‐α (1 ng/ml) exposure on the components of insulin signaling pathway in HepG2 and HepG2 cells overexpressing constitutively active Akt1/PKB‐α (HepG2‐CA‐Akt/PKB) have been investigated. In parental HepG2 cells, TNF‐α treatment for 24 h reduced the phosphorylation of Akt1/PKB‐α and GSK‐3β and under these conditions cells also showed reduced insulin responsiveness in terms of Akt1/PKB‐α and GSK‐3β phosphorylation. TNF‐α pre‐incubated HepG2‐CA‐Akt/PKB cells showed lower reduction in Akt1/PKB‐α and GSK‐3β phosphorylation and insulin responsiveness after 24 h as compared to parental HepG2 cells. We report that the long‐term TNF‐α pre‐incubation in both parental HepG2 and HepG2‐CA‐Akt/PKB‐α cells leads to the reduction in the levels of IRS‐1 without altering the levels of IRS‐2. In order to understand the reason for the differential insulin resistance in both the cell types, the effect of long‐term TNF‐α treatment on the proteins upstream to Akt/PKB was investigated. TNF‐α pre‐incubation also showed reduced insulin‐stimulated Tyr phosphorylation of insulin receptor (IR‐β) in both the cell types, moreover hyperphosphorylation of IRS‐1 at Ser 312 residue was observed in TNF‐α pre‐incubated cells. As hyperphosphorylation of IRS‐1 at Ser 312 can induce its degradation, it is possible that reduced insulin responsiveness after long‐term TNF‐α pre‐incubation observed in this study is due to the decrease in IRS‐1 levels. J. Cell. Biochem. 100: 593–607, 2007. © 2006 Wiley‐Liss, Inc.
Nucleo‐cytoplasmic cycling of the vitamin D receptor in the enterocyte‐like cell line, Caco‐2
Authors: Klopot Anna , Hance Kenneth W. , Peleg Sara , Barsony Julia , Fleet James C. 
Published: September 2006

AbstractWe examined the effects of 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) on the distribution and mobility of the vitamin D receptor (VDR) in the enterocyte‐like Caco‐2 cell. Confocal microscopy showed that a green fluorescent protein‐vitamin D receptor (GFP‐VDR) fusion protein is predominantly nuclear (58%) and it does not associate with the apical or basolateral membrane of proliferating or polarized, differentiated cells. In contrast to the previously studied cell types, neither endogenous VDR nor GFP‐VDR levels accumulate in the nucleus following 1,25(OH)2D3 treatment (100 nM, 30 min). However, in nuclear photobleaching experiments nuclear GFP‐VDR import was significantly increased by 1,25(OH)2D3 during both an early (0–5 min) and later (30–35 min) period (20% per 5 min). Compared to the natural ligand, nuclear import of GFP‐VDR was 60% lower in cells treated with the 1,25(OH)2D3 analog, 1‐alpha‐fluoro‐16‐ene‐20‐epi‐23‐ene‐26,27‐bishomo‐25‐hydroxyvitamin D3 (Ro‐26‐9228, 5 min, 100 nM). Downstream events like ligand‐induced association of VDR with chromatin at 1 h and the accumulation of CYP24 mRNA were significantly lower in Ro‐26‐9228 treated cells compared to 1,25(OH)2D3 (60 and 95% lower, respectively). Collectively our data are consistent with a role for ligand‐induced nuclear VDR import in receptor activation. In addition, ligand‐dependent VDR nuclear import appears to be balanced by export, thus accounting for the lack of nuclear VDR accumulation even when VDR import is significantly elevated. J. Cell. Biochem. 100: 617–628, 2007. © 2006 Wiley‐Liss, Inc.
Effects of a vitamin D3 analog on diabetes in the bio breeding (BB) rat
Authors: Pedullà Marcella , Desiderio Vincenzo , Graziano Antonio , d'Aquino Riccardo , Puca Andrew , Papaccio Gianpaolo 
Published: September 2006

AbstractNon‐hypercalcemic analogs of vitamin D3 modulate the immune response through antigen‐presenting cells (APCs) and activated T‐cells. A large population‐base case‐control showed that vitamin D3 intake significantly decreases the risk of type 1 diabetes development. The aim of this study was, therefore, to observe the in vivo effects of a vitamin D3 analog administered to Bio Breeding (BB) rats. 1,25‐Dihydroxy‐16,23Z‐diene‐26,27‐hexafluoro‐19‐nor vitamin D3 (BXL‐219, formerly Ro 26‐2198) (BioXell, Milan, Italy) was administered in vivo to BB rats from days 42 to 110 of life at 0.2 µg/Kg BW. Control animals received only vehicle (olive oil, 4.8 µl/100 g BW). The animals of these two groups were subjected to insulin treatment as they became diabetic. Insulin (Humulin, 28.6 UI/day) was administered irrespective of diabetes occurrence to another group of rats for comparison. Blood glucose, insulin levels, glycosuria, degree of islet infiltration, and the expression of some antigens were observed. Results showed that the vitamin D3 analog reduced diabetes incidence, although limitedly, in BB rats while administration of oral insulin increased diabetes incidence. In addition, the vitamin D3 analog did not stimulate an enhancement in the expression of CD4 and CD25 in BB rats as it does in NOD mice, which may explain the failure of this as well as other antidiabetic treatments in the BB animal model of type 1 diabetes. J. Cell. Biochem. 100: 808–814, 2007. © 2006 Wiley‐Liss, Inc.
Jailed resources: conservation of resources theory as applied to burnout among prison guards
Authors: Neveu Jean‐Pierre 
Published: September 2006

AbstractThis study evaluates a salutogenic perspective of the burnout process. Building upon Hobfoll's (1989) Conservation of Resources theory, it proposes a simultaneous test of three hypothesized resources‐based models. These competing models test the structure of burnout in relation to depleted resources (e.g., lack of skill utilization, of participation, of co‐worker support, and of professional worth) and negative correlates (e.g., absenteeism and depression). SEM results provide equally good support for two resource‐based models, although each of them proceeds from two different approaches (Leiter vs. Golembiewski). Of all burnout components, personal accomplishment is found to be least related to resources depletion, while emotional exhaustion is the most related to absenteeism and depression. Results are analyzed in light of existing literature and of the specific nature of the sample, a large population of French correctional officers (n = 707). Implications for burnout theory and human resource management are discussed. Copyright © 2006 John Wiley & Sons, Ltd.
Effects of rivastigmine in patients with and without visual hallucinations in dementia associated with Parkinson's disease
Authors: Burn David , Emre Murat , McKeith Ian , De Deyn Peter Paul , Aarsland Dag , Hsu Chuanchieh , Lane Roger 
Published: September 2006

AbstractWe aimed to determine prospectively whether rivastigmine, an inhibitor of acetylcholinesterase and butyrylcholinesterase, provided benefits in patients with and without visual hallucinations in a population with dementia associated with Parkinson's disease (PDD). This was a 24‐week double‐blind placebo‐controlled study. Primary efficacy measures were the Alzheimer's Disease Assessment Scale cognitive subscale (ADAS‐cog) and Alzheimer's Disease Cooperative Study–Clinician's Global Impression of Change (ADCS‐CGIC). Secondary efficacy measures included activities of daily living, behavioral symptoms, and executive and attentional functions. Patients were stratified according to the presence of visual hallucinations at baseline. The study included 188 visual hallucinators (118 on rivastigmine, 70 on placebo) and 348 nonvisual hallucinators (239 on rivastigmine, 109 on placebo). Rivastigmine provided benefits in both visual hallucinators and nonvisual hallucinators. Absolute responses to rivastigmine on the ADAS‐cog were comparable over 6 months, although rivastigmine–placebo differences tended to be larger in visual hallucinators (4.27; P = 0.002) than in nonhallucinators (2.09; P = 0.015). On the ADCS‐CGIC, differences between rivastigmine and placebo were 0.5 in visual hallucinators (P = 0.030) and 0.3 in nonhallucinators (P = 0.111). Rivastigmine provided benefits on all secondary efficacy measures, and placebo declines and treatment differences were more marked in visual hallucinators. Adverse events were reported more frequently by rivastigmine‐treated patients, although this difference was less marked in visual hallucinators. Visual hallucinations appear to predict more rapid decline and possibly greater therapeutic benefit from rivastigmine treatment in PDD. © 2006 Movement Disorder Society
Correlation between cardiac 123I‐MIBG and odor identification in patients with Parkinson's disease and multiple system atrophy
Authors: Lee Phil Hyu , Yeo Seung Hyeon , Kim Hyun Joon , Youm Hyo Young 
Published: September 2006

AbstractWe investigated an association between olfaction and cardiac 123I‐metaiodobenzylguanidine (MIBG) uptake in patients with Parkinson's disease (PD) and multiple system atrophy (MSA). There was a significant positive correlation between cardiac MIBG uptake and the Cross‐Cultural Smell Identification (CCSI) score in patients with PD (r = 0.56; P = 0.003) independent of the disease duration or clinical rating of motor status. However, patients with MSA did not show a significant correlation between cardiac MIBG uptake and the CCSI score. Our findings suggest that the functional losses of the olfactory and cardiac sympathetic systems are closely coupled in PD. © 2006 Movement Disorder Society
Distinction of Tris(diimine)ruthenium(II) enantiomers chiral by virtue of helical chirality: temperature‐dependent deuterium NMR spectroscopy in partially oriented phases
Authors: Szalontai Gábor , Kovács Margit 
Published: September 2006

Abstract2H NMR spectra of perdeuterated tris(diimine)ruthenium(II) complexes have been recorded in lyotropic liquid crystalline phase formed by the chiral polypeptide, poly‐γ‐benzyl‐L‐glutamate (PBLG) and co‐solvents. It is demonstrated that the left‐ and right‐rotation isomers of these octahedral metal complexes with D3 symmetry can be distinguished. The effects of temperature and ligand size on spectral resolution were also studied. Although in the case of free bipyridine ligands excellent optical resolution could be obtained at room temperature in the complexes studied, the increase in ligand size has a detrimental effect on the resolution. This can be compensated to some extent by elevating the sample temperature and decreasing the deuterium relaxation rates, but the available temperature range and therefore the applicability of the technique are limited. Copyright © 2006 John Wiley & Sons, Ltd.
29Si spin‐lattice NMR relaxation in microporous silica‐based materials with high Mn2+ concentrations
Authors: Bakhmutov Vladimir I. , Shpeizer Boris G. , Clearfield Abraham 
Published: September 2006

AbstractThe 29Si T1 time measurements have been performed under magic angle spinning for supermicroporous Si/Mn materials 1–6 containing Mn2+ in concentrations of 0 (1), 1.2 (2), 2.5 (3), 3.8 (4), 15 (5) and 19.8 wt% (6). It has been found that the central lines, corresponding to isotropic chemical shifts, and their sidebands relax differently in samples 2–4 with relatively small Mn2+ contents. Because the relaxation curves for 1–6 are nonexponential and identical for 6 at spinning rates of 5, 10 and 12 kHz, dipolar relaxation via the paramagnetic centers is dominant. The relaxation data have been interpreted as a function of Mn2+ concentrations in terms of incorporation of the Mn2+ ions into the silica lattice when their concentration is small (≤2.5 wt%). At higher concentrations, most of the manganese is located on the surface of the silica. Copyright © 2006 John Wiley & Sons, Ltd.