bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix metalloproteinase‐2 and ‐9 activities in human keloids, hypertrophic and atrophic scars: a pilot study
Authors: Tanriverdi‐Akhisaroglu S. , Menderes A. , Oktay G. 
Published: January 2009

AbstractProteolytic degradation of extracellular matrix is one of the principal features of cutaneous wound healing but little is known about the activities of gelatinases; matrix metalloproteinase‐2 (MMP‐2) and matrix metalloproteinase‐9 (MMP‐9) on abnormal scar formation. The aim of this study is to determine collagen levels and the gelatinase activities in tissue from hypertrophic scars, atrophic scars, keloids and donor skin in 36 patients and 14 donors. Gelatinase levels (proenzyme + active enzyme) were determined by ELISA and their activities by gelatin zymography. MMP‐9 activity was undetectable in gelatin zymography analysis. Pro‐MMP‐2 levels (median) were highest in normal skin group 53.58 (36.40–75.11) OD µg−1 protein, while active MMP‐2 levels were highest in keloid group 52.53 (42.47–61.51) OD µg−1 protein. The active/pro ratio was the highest in keloid group 0.97 followed by hypertrophic scar, normal skin and atrophic scar groups 0.69 > 0.54 > 0.48, respectively. According to results of our study, the two‐phase theory of the duration of hypertrophic scar and keloid formation can be supported by the data of tissue collagen and gelatinase analysis. This study is the first to relate scar formation relationship in regard to gelatinase activation ratio in a keloid, hypertrophic and atrophic scar patient group which is chosen appropriate in age and sex. Copyright © 2009 John Wiley & Sons, Ltd.
Experience with the Cardiva Boomerang Catalyst™ system in pediatric cardiac catheterization
Authors: Seltzer Sharon , Alejos Juan Carlos , Levi Daniel S. 
Published: January 2009

AbstractObjectives: We studied the safety and efficacy of the Cardiva Boomerang Catalyst™ vascular closure system in pediatric patients after cardiac catheterization with access in femoral and internal jugular vessels. Background: Recurrent catheterization and advances in pediatric interventions increase the need for easy hemostasis without a residual foreign body that may prevent re‐accessing the vessel. The Boomerang can be deployed in sheaths as small as 4Fr without residual foreign body, with minimal orientation needed, and few complications reported. Methods: In a two‐month period, all patients between 18 months and 21 years old catheterized with 4–8Fr sheaths less than 15 cm long were eligible for Boomerang placement. These were compared retrospectively with control patients with manual hemostasis. Anthropomorphic measurements, procedure type, activated clotting time, and sheath size as well as total times of cases, intubation, hemostasis, and extubation were compared between the two groups. Results: Forty‐six Boomerangs were deployed in 31 patients and compared with 40 patients with manual hemostasis. Boomerangs were deployed in femoral vessels and the internal jugular vein. Device success with hemostasis was achieved in 39 patients (85%). There were no significant differences in time to hemostasis or extubation between the two groups. No major complications or operator error occurred, including hematoma, transfusion, retroperitoneal bleed, infection, vessel occlusion, or need for surgery. Conclusions: The Boomerang is a safe and easy means of achieving hemostasis in the pediatric population, in femoral vessels as well as internal jugular veins. Its times to hemostasis and extubation were not significantly different from manual hold. © 2009 Wiley‐Liss, Inc.
Copper(II) Biosorption on Soda Lignin From Oil Palm Empty Fruit Bunches (EFB)
Authors: Ibrahim Mohamad Nasir Mohamad , Ngah Wan Saime Wan , Norliyana Mohd Salleh , Daud Wan Rosli Wan 
Published: January 2009

AbstractThe adsorption of Cu(II) ions from aqueous solutions by soda lignin as an absorbent using a batch adsorption system is presented in this paper. The soda lignin used in this study was extracted from black liquor derived from oil palm empty fruit bunches (EFB) using 20% v/v sulfuric acid. The effects of varying experimental parameters such as pH value, adsorbent dosage, different concentrations of Cu(II) ions, and agitation period were investigated. The results revealed that the optimum adsorption of Cu(II) onto soda lignin was recorded at a pH of 5.0 at an adsorbent dosage of 0.5 g soda lignin and an agitation period of 40 min. The adsorption capacities and rates of Cu(II) ions onto soda lignin was evaluated. The Langmuir and Freundlich adsorption models were applied to calculate the isotherm constants. It was found that the adsorption isothermal data could be well interpreted by the Freundlich model. The kinetic experimental data properly correlated with the pseudo‐second‐order kinetic model, which implies that chemical sorption is the rate‐limiting step.
Low positive predictive value of the oral brush biopsy in detecting dysplastic oral lesions
Authors: Bhoopathi Vinodh , Kabani Sadru , Mascarenhas Ana Karina 
Published: January 2009

AbstractBACKGROUND:The authors evaluated the effectiveness of the oral brush biopsy technique as a diagnostic tool in detecting dysplastic oral lesions.METHODS:In this cross‐sectional study, pathologic reports (n = 152) from the scalpel biopsies (tissue samples) in patients who previously tested either “positive” (n = 3) or “atypical” (n = 149) for dysplasia by brush biopsy (OralCDx) were evaluated. Information on the age and sex of the patient, the site of the lesion, the brush biopsy results, and the histopathologic diagnosis of the scalpel biopsy was collected. The positive predictive values (PPVs) for “abnormal,” “atypical,” and “positive” brush biopsies were determined.RESULTS:Overall, the PPV of an abnormal brush biopsy was only 7.9% (95% confidence interval [CI], 4.2%‐13.4%), and the PPV of an “atypical” brush biopsy was 7.4% (95% CI, 3.7%‐12.8%). Of the 3 positive brush biopsies, only 1 was identified as dysplastic. The proportion of false‐positive biopsy results was as high as 92.1% (95% CI, 86.6%‐95.9%).CONCLUSIONS:The OralCDx technique overestimated dysplastic lesions and produced a high number of false‐positive results. Cancer 2009. © 2009 American Cancer Society.
Routine perioperative chemotherapy instillation with initial bladder tumor resection
Authors: Rao Pravin K. , Stephen Jones J. 
Published: January 2009

AbstractBACKGROUND:Level‐1 evidence has demonstrated decreased recurrence of low‐grade bladder tumors when initial transurethral resection (TUR) is followed by perioperative instillation (PI) of chemotherapy. A meta‐analysis determined that the number needed to treat (NNT) was 8.5 patients to prevent 1 recurrence. No benefit was demonstrated for tumors classified as T0, tumor in situ, or T2; thus, patients with those tumors were excluded from the analysis, which potentially may have resulted in underestimating the true NNT. Economic benefits were suggested, but cost calculations were not presented. The objectives of the current analysis were to recalculate the NNT considering patients who previously were excluded and to examine the economic implications based on various management alternatives for tumor recurrence.METHODS:For each study that was included in the current meta‐analysis, the number of patients excluded because of ‘inappropriate’ pathology results was determined. A potentially more accurate NNT was calculated, and pertinent Medicare reimbursements were obtained to estimate costs.RESULTS:The added cost for 8.5 patients who underwent inpatient TUR to receive PI was $1711. Inpatient TUR ($7025) was extremely costly compared with hospital outpatient TUR ($2666), ambulatory surgery center TUR ($2113), and physician office fulguration ($1167). Although the inclusion of patients who previously were excluded resulted in a recalculated NNT of 9.6 patients, the authors used a more conservative NNT if 8.5 patients to estimate the economic impact of the ‘best‐case scenario.’CONCLUSIONS:Routine PI significantly lowered the overall cost if recurrences were managed in the inpatient setting, but these benefits were offset mostly or completely by outpatient management in the United States. Thus, the authors concluded that the decision to use routine PI of chemotherapy should be based on clinical effects and not on presumed economic benefits. Cancer 2009. © 2009 American Cancer Society.
A multifunctional reporter mouse line for Cre‐ and FLP‐dependent lineage analysis
Authors: Yamamoto Masakazu , Shook Nicole A. , Kanisicak Onur , Yamamoto Shoko , Wosczyna Michael N. , Camp James R. , Goldhamer David J. 
Published: January 2009

AbstractThe Cre/lox and FLP/FRT recombination systems have been used extensively for both conditional knockout and cell lineage analysis in mice. Here we report a new multifunctional Cre/FLP dual reporter allele (R26NZG) that exhibits strong and apparently ubiquitous marker expression in embryos and adults. The reporter construct, which is driven by the CAG promoter, was knocked into the ROSA26 locus providing an open chromatin domain for consistent expression and avoiding site‐of‐integration effects often observed with transgenic reporters. R26NZG directs Cre‐dependent nuclear‐localized β‐galactosidase (β‐gal) expression, and can be converted into a Cre‐dependent EGFP reporter (R26NG) by germline excision of the FRT‐flanked nlslacZ cassette. Alternatively, germline excision of the floxed PGKNEO cassette in R26NZG generates an FLP‐dependent EGFP reporter (R26ZG) that expresses β‐gal in FLP‐nonexpressing cells. Finally, by the simultaneous use of both Cre and FLP deleters, R26NZG allows lineage relationships to be interrogated with greater refinement than is possible with single recombinase reporter systems. genesis 47:107–114, 2009. © 2009 Wiley‐Liss, Inc.
Synthesis of Phenanthro[9,10‐b]indolizidin‐9‐ones, Phenanthro[9,10‐b]quinolizidin‐9‐one, and Related Benzolactams by Pd(OAc)2‐Catalyzed Direct Aromatic Carbonylation
Authors: Yamashita Satoshi , Kurono Nobuhito , Senboku Hisanori , Tokuda Masao , Orito Kazuhiko 
Published: January 2009

AbstractPhenanthro[9,10‐b]indolizidin‐9‐ones, phenanthro[9,10‐b]quinolizidin‐9‐one, and related benzolactams were synthesized by benzolactam ring formation using Pd(OAc)2‐catalyzed direct aromatic carbonylation. This also constitutes a formal synthesis of the representative phenanthroindolizidine and ‐quinolizidine alkaloids (±)‐tylophorine, (±)‐antofine, and (±)‐cryptopleurine.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Palladium‐Catalyzed Three‐Component Coupling Reactions: 1,1‐Difunctionalization of Activated Alkenes
Authors: Rodriguez Arantxa , Moran Wesley J. 
Published: January 2009

AbstractA three‐component coupling reaction was developed to access 3,3‐oxyarylpropionate derivatives. Each of the three reaction components can be varied, allowing the modular synthesis of a range of important chiral building blocks. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Electrochemical Study of Anionic Ferrocene Derivatives Intercalated in Layered Double Hydroxides: Application to Glucose Amperometric Biosensors
Authors: Mousty Christine , Forano Claude , Fleutot Solenne , Dupin Jean‐Charles 
Published: January 2009

AbstractLayered double hydroxides (Zn2Cr(OH)6X⋅nH2O LDH) containing (3‐sulfopropyl)ferrocene‐carboxylate (FcPSO3) and 1,1′‐bis(3‐sulfopropyl)ferrocene‐carboxylate (Fc(PSO3)2) as interlayer anions (X) have been prepared by the co‐precipitation method and characterized by PXRD, FTIR, SEM and XPS. The electrochemical behavior of these hybrid materials has been evaluated by cyclic voltammetry. A new amperometric biosensor based on the immobilization of glucose oxidase in ZnCr‐FcPSO3 hybrid material was presented, the intercalated anions playing the role of mediators that shuttle electrons between the FAD centers in the enzyme and the electrode surface. The performance of the resulting biosensor for glucose determination under anaerobic conditions was evaluated by chronoamperometry at 0.5 V. The sensitivity (65 mA M−1 cm−2) determined in the concentration range 10–25 μM is higher than sensitivities reported for other glucose biosensors based on LDH host matrices.
Hybrid Microfluidic Sensors Fabricated by Screen Printing and Injection Molding for Electrochemical and Electrochemiluminescence Detection
Authors: Redha Zainab Mohammed , Baldock Sara. J. , Fielden Peter R. , Goddard Nick J. , Brown Bernard J. Treves , Haggett Barry G. D. , Andres Roberto , Birch Brian J. 
Published: January 2009

AbstractAn integrated microfluidic/electrochemical sensor with a dual working electrode configuration was fabricated for voltammetric and electrochemiluminescence (ECL) applications. The fabrication method integrates the two techniques of injection molding and screen printing for the first time. Injection molding is used to fabricate the flow cell with integrated electrical and fluidic connectors. Screen printing was used to print the electrodes directly onto the injection molded base plate. An over molding procedure followed by ultrasonic welding was used to form the complete sensor. The electrochemical and ECL performance of the sensors was assessed by applying different electrochemical methods such as cyclic voltammetry (CV), anodic stripping voltammetry (ASV) and chronoamperometry. The attractive analytical performance demonstrates good capability of the disposable sensors for routine on‐line electrochemical and ECL measurements.
What Do We Know about Determination of Amino Acids with Orthophthalaldehyde?
Authors: Zuman Petr , Salem Nuha , Kulla Eliona 
Published: January 2009

AbstractMechanisms of the formation of the fluorescent species widely used for determination of amino acids, based on reaction with orthophthalaldehyde (OPA) were unknown. Attempts to elucidate this mechanism based solely on product identification were unsuccessful. Analytical methods based on reaction of OPA with amino acids in the presence of RS− or CN− ions were developed empirically. The reaction conditions reported in more than 700 papers differed considerably. In aqueous solutions OPA exists in three different forms in equilibrium – the unhydrated form Ia (20%), monohydrated acyclic form Ib (8%) and cyclic hemiacetal form Ic (72%). These forms are in equilibria, the rate of establishment of which is acid and base catalyzed. Reactions of OPA and NH3 and 2‐aminoethanol (colamine) take place in a series of equilibria depicted in Scheme 1. The isoindole derivatives are formed in cyclization of the carbinolamine. Formation of an imine is a side reaction.
Interaction of Biomacromolecules with Surfaces Viewed by Electrochemical Methods
Authors: Dorčák V. , Bartošík M. , Ostatná V. , Paleček E. , Heyrovský M. 
Published: January 2009

AbstractThe electrocatalytic evolution of hydrogen on mercury electrodes by organic molecules indicates, when followed with chronopotentiometric stripping method by the ‘peak H’, that when the stripping current is changed, the mechanism of the electrode process changes. The changes become the more prominent the larger is the catalyzing organic molecule. We explain this phenomenon by dynamic interaction of the catalyzing molecule with the electrode surface.
Biosensors Based on Poly(vinylimidazole) Microparticles
Authors: Sánchez‐Paniagua López M. , López‐Cabarcos E. , López Ruiz B. 
Published: January 2009

AbstractThe use of poly(vinylimidazole) microparticles as immobilization system of the enzymes used as biological material in the fabrication of glucose and catechol biosensors is proposed. Biosensors analytical properties were studied using different working media: aqueous buffer solution and solvents with different hydrophobicity such as dioxane (lgP=−1.1), acetonitrile (lgP=−0.33), ethanol (lgP=−0.24), chloroform (lgP=2.0) and hexane (lgP=3.5) as well as different mixtures of acetonitrile/aqueous buffer solution. Sensitivity, detection limit, linear range and response time were reported for both biosensors in both media. The performance of the biosensors in the analysis of glucose and phenols in real samples was successful.
A hypothetical reality of Tarrawarra‐like hydrologic response
Authors: Mirus Benjamin B. , Loague Keith , VanderKwaak Joel E. , Kampf Stephanie K. , Burges Stephen J. 
Published: January 2009

AbstractThe objective of this work was to develop a hypothetical reality of hydrologic response to be used as an error‐free synthetic dataset in a larger ongoing study. The hypothetical reality was generated via rigorous simulation of a real system with a comprehensive physics‐based model. The simulation was conducted with the Integrated Hydrology Model (InHM); the system is the Tarrawarra catchment located in southeastern Australia. Parameterization of the Tarrawarra boundary‐value problem was based on the best available information, which, for example includes rainfall, discharge, and soil‐water content data for the last six months of 1996. The InHM‐simulated near‐surface hydrologic response for the Tarrawarra boundary‐value problem compares well (albeit not perfectly) with both the integrated and distributed observations from the catchment. The Tarrawarra‐like hypothetical reality of wet season hydrologic response generated in this study is rich enough to be employed as an error‐free synthetic dataset for quantitatively evaluating the capabilities/limitations of competing (underlying) modelling techniques. Copyright © 2009 John Wiley & Sons, Ltd.
Population‐based study of autoimmune conditions and the risk of specific lymphoid malignancies
Authors: Anderson Lesley A. , Gadalla Shahinaz , Morton Lindsay M. , Landgren Ola , Pfeiffer Ruth , Warren Joan L. , Berndt Sonja I. , Ricker Winnie , Parsons Ruth , Engels Eric A. 
Published: January 2009

AbstractSome autoimmune conditions are associated with increased risk of lymphoid malignancies, but information on specific malignancy subtypes is limited. From the U.S. Surveillance Epidemiology and End Results‐Medicare database, we selected 44,350 lymphoid malignancy cases (≥67 years) and 122,531 population‐based controls. Logistic regression was used to derive odds ratios (ORs) comparing the prevalence of autoimmune conditions in cases and controls, by lymphoid malignancy subtype, adjusted for gender, age at malignancy/selection, year of malignancy/selection, race and number of physician claims. The strongest associations observed by non‐Hodgkin lymphoma (NHL) subtypes were diffuse large B‐cell lymphoma with rheumatoid arthritis (OR 1.4, 95%CI 1.2–1.5) and Sjögren syndrome (2.0, 1.5–2.8); T‐cell lymphoma with hemolytic anemia (9.7, 4.3–22), psoriasis (3.1, 2.5–4.0), discoid lupus erythematosus (4.4, 2.3–8.4) and celiac disease (5.0, 2.4–14.); and marginal zone lymphoma with Sjögren syndrome (6.6, 4.6–9.5), systemic lupus erythematosus (2.8, 1.7–4.7) and hemolytic anemia (7.4, 3.1–18). Hodgkin lymphoma was associated with systemic lupus erythematosus (3.5, 1.9–6.7). Multiple myeloma was associated only with pernicious anemia (1.5, 1.3–1.7). Several autoimmune conditions were associated with increased risk of lymphoid neoplasms, especially NHLs of diffuse large B‐cell, marginal zone and T‐cell subtypes. These results support a mechanism whereby chronic antigenic stimulation leads to lymphoid malignancy. Published 2009 UICC.
Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B‐Raf
Authors: Zhang Hui‐Hua , Walker Francesca , Kiflemariam Sara , Whitehead Robert H. , Williams David , Phillips Wayne A. , Mikeska Thomas , Dobrovic Alexander , Burgess Antony W. 
Published: January 2009

AbstractTumor‐derived cell lines are indispensable tools for understanding the contribution of activated signaling pathways to the cancer phenotype and for the design and testing of targeted signal therapies. In our study, we characterize 10 colorectal carcinoma cell lines for the presence of mutations in the wnt, Ras/MAPK, PI3K and p53 pathways. The mutational spectrum found in this panel of cell lines is similar to that detected in primary CRC, albeit with higher frequency of mutation in the β‐catenin and B‐Raf genes. We have monitored activation of the wnt and Ras/MAPK pathways in these cells and analyzed their sensitivity to selective signaling inhibitors. Using β‐catenin subcellular distribution as a marker, we show that cells harboring APC mutations have low‐level activated wnt signaling, which can be blocked by the extracellular wnt inhibitor DKK‐1, suggesting autocrine activation of this pathway; proliferation of these cells is also blocked by DKK‐1. In contrast, cells with β‐catenin mutations are unresponsive to extracellular wnt inhibition. Constitutive phosphorylation of MAPK is present in the majority of the cell lines and correlates with B‐Raf but not K‐Ras mutations; correspondingly, the proliferation of cells harboring mutations in B‐Raf, but not K‐Ras, is exquisitely sensitive inhibition of the MAPK pathway. We find no correlation between PI3K mutation or loss of PTEN expression and increased sensitivity to PI3K inhibitors. Our study discloses clear‐cut differences in responsiveness to signaling inhibitors between individual mutations within an activated signaling pathway and suggests likely targets for signal‐directed therapy of colorectal carcinomas. © 2009 UICC
Survivin minigene DNA vaccination is effective against neuroblastoma
Authors: Fest Stefan , Huebener Nicole , Bleeke Matthias , Durmus Tahir , Stermann Alexander , Woehler Anja , Baykan Bianca , Zenclussen Ana C. , Michalsky Elke , Jaeger Ines S. , Preissner Robert , Hohn Oliver , Weixler Silke , Gaedicke Gerhard , Lode Holger N. 
Published: January 2009

AbstractThe inhibitor of apoptosis protein survivin is highly expressed in neuroblastoma (NB) and survivin‐specific T cells were identified in Stage 4 patients. Therefore, we generated a novel survivin minigene DNA vaccine (pUS‐high) encoding exclusively for survivin‐derived peptides with superior MHC class I (H2‐Kk) binding affinities and tested its efficacy to suppress tumor growth and metastases in a syngeneic NB mouse model. Vaccination was performed by oral gavage of attenuated Salmonella typhimurium SL7207 carrying pUS‐high. Mice receiving the pUS‐high in the prophylactic setting presented a 48–52% reduction in s.c. tumor volume, weight and liver metastasis level in contrast to empty vector controls. This response was as effective as a survivin full‐length vaccine and was associated with an increased target cell lysis, increased presence of CD8+ T‐cells at the primary tumor site and enhanced production of proinflammatory cytokines by systemic CD8+ T cells. Furthermore, depletion of CD8+ but not CD4+ T‐cells completely abrogated the pUS‐high mediated primary tumor growth suppression, demonstrating a CD8+ T‐cell mediated effect. Therapeutic vaccination with pUS‐high led to complete NB eradication in over 50% of immunized mice and surviving mice showed an over 80% reduction in primary tumor growth upon rechallenge in contrast to controls. In summary, survivin‐based DNA vaccination is effective against NB and the rational minigene design provides a promising approach to circumvent potentially hazardous effects of using full length antiapoptotic genes as DNA vaccines. © 2009 UICC