bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adhesive bond strength of end grain joints in softwood with varying density
Authors: Follrich Jürgen , Teischinger Alfred , Gindl Wolfgang , Müller Ulrich 
Published: January 2008

Abstract
Norway spruce wood specimens covering a wide density range, including density samples between 300 kg m-3 (low density wood) and 750 kg m-3 (high-density compression wood), were joined at end grain surfaces and tested in tension perpendicular to the bonded surface to estimate the effect of mechanical interlocking on the adhesive bond strength. It was hypothesised that the higher the density of the specimens the lower will be the mechanical interlocking of the adhesive joints due to a reduced penetration of the adhesive into the cell cavities. An increase of tensile strength was observed with increasing density. These results demonstrate that in the specific case investigated here the cross-sectional cell wall area available for adhesive bonding, which correlates with density, is probably more important than mechanical interlocking.
Laccase-catalyzed functionalization with 4-hydroxy-3-methoxybenzylurea significantly improves internal bond of particle boards
Authors: Fackler Karin , Kuncinger Thomas , Ters Thomas , Srebotnik Ewald 
Published: January 2008

Abstract
Enzymatic functionalization is an attractive tool to provide a reactive interface for further processing of lignocellulosic materials, such as wood particles and fibers. Here, spruce wood particles have been functionalized by fungal laccase combined with 4-hydroxy-3-methoxy-benzylamine (HMBA) or 4-hydroxy-3-methoxybenzylurea (HMBU). The expectation was crosslinking with resins in subsequent glueing processes, which should improve strength properties of particle boards. Essential process parameters, such as liquid to solid mass ratio and treatment time, were optimized on a laboratory scale resulting in HMBA and HMBU binding yields of 90% and above as determined by radiochemical mass balance analysis. We employed a multifactorial experimental design for board production from treated wood particles and urea/formaldehyde resin. Mechanical testing and multivariate data analysis revealed, for the first time, an increase of internal bond (IB) as a result of functionalization with HMBU. HMBA was not successful. Variance analysis of relevant parameters and their interactions demonstrated a highly significant difference (P>99.99%) between boards treated with laccase/HMBU versus untreated wood particles. Due to positive interactions, functionalization was most effective at high bulk density (750 kg m-3) and high resin content (10%) resulting in a calculated IB improvement of 0.12 N m-2 (21%).
Morphologic and Management Characteristics of Individuals with Unilateral Cleft Lip and Palate Who Required Maxillary Advancement
Authors: Oberoi Snehlata , Chigurupati Radhika , Vargervik Karin 
Published: January 2008

Objective: To delineate factors that may contribute to maxillary hypoplasia requiring maxillary advancement surgery in individuals with nonsyndromic unilateral cleft lip and palate (UCLP). Methods: This retrospective, longitudinal study used lateral cephalometric radiographs and chart reviews of 16 nonsyndromic UCLP individuals who underwent Le Fort I maxillary advancement and 16 controls matched for cleft type, age, and gender. Cephalometric measurements were made at three time points (T1, T2, and T3): mean ages of 10.7, 13.3, and 15.8 years for the Le Fort group and 10.11, 12.9, and 15.7 years, respectively, for the control group. Information regarding team care, timing and number of surgical procedures, and number of congenitally missing teeth were determined from clinical records. Results: The Le Fort group had significant maxillary hypoplasia at all time points compared to the UCLP controls, indicated by midface length measurements, ANB and Wit's analysis ( p < .001). The Le Fort group had twice the number of palatal surgical procedures and number of missing teeth in the maxillary arch as compared with the cleft controls. Most of the control group had consistent team care, while most of the surgical group did not. Conclusions: Maxillary hypoplasia that will require a Le Fort I advancement can be determined as early as age 10. Multiple missing maxillary teeth, secondary palate procedures including pharyngeal flaps, and inconsistent team care with delayed orthodontic intervention are contributing factors to maxillary underdevelopment.
Early Intervention for Speech Impairment in Children with Cleft Palate
Authors: Scherer Nancy J. , D'Antonio Linda L. , McGahey Holly 
Published: January 2008

Objective: This study explored the effectiveness of a parent-implemented, focused stimulation program on the speech characteristics of children younger than 3 years with cleft lip and palate. The research questions included the following: (1) Can parents be trained to deliver an early intervention (EI) program for children with cleft palate? (2) Does a parent-implemented EI program result in positive changes in speech characteristics? Participants: Ten mother-child pairs in which the child had cleft lip and palate (CLP) and 10 mother-child pairs in which the child did not have a cleft (NCLP). The children ranged in age from 14 to 36 months of age and were matched between the CLP and the NCLP groups for vocabulary size, age, and socioeconomic status. Main Outcome Measures: Group differences (CLP and the NCLP) for preintervention and postintervention language and speech measures were compared. Results: The results of this study showed that the mothers could be trained to deliver the intervention reliably. Furthermore, the results indicated that the intervention resulted in increased sound inventories, increased speech accuracy, and reduced use of glottal stops for the children with clefts. Conclusions: While the intervention resulted in speech gains for the children with clefts, speech measures did not exceed those made by the children without clefts. The results of the study have implications for service delivery models where the services of speech-language pathologists are limited.
Universal Parameters for Reporting Speech Outcomes in Individuals with Cleft Palate
Authors: Henningsson Gunilla , Kuehn David P. , Sell Debbie , Sweeney Triona , Trost-Cardamone Judith E. , Whitehill Tara L. 
Published: January 2008

Objective: To achieve consistency and uniformity in reporting speech outcomes in individuals born with cleft palate with or without cleft lip using perceptual parameters that characterize their speech production behavior regardless of the language or languages spoken. Design: A working group of six individuals experienced in speech and cleft palate was formed to develop a system of universal parameters for reporting speech outcomes in individuals born with cleft palate. The system was adopted in conjunction with a workshop held in Washington, D.C., that was devoted to developing the universal system. The system, which was refined further following the workshop, involves a three-stage plan consisting of (1) evaluation, (2) mapping, and (3) reporting. The current report focuses primarily on the third stage, reporting speech outcomes. Results: A set of five universal speech parameters has been devised for the reporting stage. These consist of (1) hypernasality, (2) hyponasality, (3) audible nasal air emission and/or nasal turbulence, (4) consonant production errors, and (5) voice disorder. Also included are speech understandability and speech acceptability, global parameters that can be reported for any type of speech disorder. The parameters are described in detail, and guidelines for speech-sampling content and scoring procedures in relation to the parameters are presented. Conclusion: A plan has been developed to document speech outcomes in individuals with cleft palate, regardless of the spoken language, using a set of five universal reporting parameters and two global speech parameters.
The Goslon Yardstick in Patients with Unilateral Cleft Lip and Palate: Review of a Vienna Sample
Authors: Sinko Klaus , Caacbay Emma , Jagsch Reinhold , Turhani Dritan , Baumann Arnulf , Mars Michael 
Published: January 2008

Objective: To compare a Vienna unilateral cleft lip and palate (UCLP) patient sample with the Eurocleft samples using the GOSLON score, to determine the intra- and interrater agreement between several raters and ratings, and to establish whether training with the original GOSLON models enhances accuracy. Patients and Methods: One hundred twenty-three plaster casts of UCLP patients born between 1970 and 1997, with an average age of 9.2 years and all treated with the same regimen, were rated according to the GOSLON score. Results: Of the patients, 71.5% were ranked GOSLON 1 or 2. Only 8.9% were rated GOSLON 4 or 5. There were no significant differences between the different raters and the ratings. Training with the original GOSLON models increased kappa from 0.57 before training to 0.84 after training. Conclusion: The “Vienna concept” was found to be a good regimen for treating UCLP patients in regard to maxillary growth. Personal training on the original GOSLON models appears to improve the accuracy of rating.
A Longitudinal Study of Speech Production in Swedish Children with Unilateral Cleft Lip and Palate and Two-stage Palatal Repair
Authors: Lohmander Anette , Persson Christina 
Published: January 2008

Objective: To describe speech production longitudinally in a group of children with unilateral cleft lip and palate (UCLP). Participants: Twenty consecutive children with UCLP and nine age-matched children without clefts in a comparison group. Intervention: A two-stage palatal repair procedure with soft palate closure at 6 months and hard palate repair at 3 to 4 years. Main Outcome Measures: Percent correct consonants (PCC), percent correct places (PCP), and percent correct manners (PCM) at 3, 5, and 7 years of age. Cleft speech errors at the same ages. Previously collected data on number of consonant tokens, consonant types, frequency of occurrence of places and manners of articulation at 18 months. Results: PCC and PCP were significantly lower in the UCLP group than in the comparison group at all ages. Number of consonant types and frequency of occurrence of dental plosives at 18 months correlated significantly with PCC at age 3. A high frequency of velar plosives at 18 months correlated significantly with a high prevalence of retracted oral articulation (dental/alveolar to palatal or velar) at both 3 and 5 years of age. Conclusions: The UCLP group performed worse than the comparison group at all ages. A high occurrence of dental plosives as well as a high number of consonant types in babbling and first words seem to be good indicators for better consonant production in later speech. The same prevalence of retracted oral articulation as in previous studies is attributed to the surgical technique.
Relationship of Premaxillary Bone and Its Sutures to Deciduous Dentition in Nonhuman Primates
Authors: Carmody K. A. , Mooney M. P. , Cooper G. M. , Bonar C. J. , Siegel M. I. , Dumont E. R. , Smith T. D. 
Published: January 2008

Objective: The relationship of the human premaxillary bone (Pmx) to neighboring craniofacial structures is clouded by its embryonic union with the maxillary bone proper. Only humans among all primates have such early fusion of the premaxillomaxillary suture (PS). This study surveyed the relationship of the PS to the upper deciduous dentition in nonhuman primates, and describes the distribution of bone cells along the osseous margins of the Pmx. Method: Twenty-eight subadult primates were studied using gross, CT, and histologic observations. Location of the anterior deciduous dentition relative to the PS was assessed. In sections of selected specimens, observations of bone cells on the osseous boundaries of the Pmx were made. Osteopontin (OPN) immunohistochemistry was used to isolate osteoclastic binding sites along the Pmx boundaries. Results: The PS was consistently found between deciduous incisor and canine in strepsirrhines of all ages, whereas the suture passed variably closer to the incisor or canine in haplorhines. In all species, the anterior part of the Pmx was nonarticulating and mostly osteoblastic, except for osteoclastic margins adjacent to dentition and the nasal fossa. Superolaterally, the osteogenic fronts of the PS were osteoblastic, while more inferiorly, at the level of the deciduous canine, the PS was often osteoclastic. Results from OPN immunohistochemistry support the findings on bone cell distribution. Conclusion: Bone cell distribution patterns in perinatal nonhuman primates resemble those described for the prenatal human Pmx, suggesting that differences among species relate to magnitude rather than the pattern of osteogenesis.
Identification of Cephalometric Landmarks in Unilateral Cleft Lip and Palate Patients: Are There Alternatives for Point A, ANS, and PNS?
Authors: Bongaarts Catharina A. M. , van't Hof Martin A. , Prahl-Andersen Birte , Kuijpers-Jagtman Anne M. 
Published: January 2008

Objective: To test the reliability of some cephalometric measurements in unilateral cleft lip and palate patients. Measurements with A, ANS, and PNS, were compared to measurements performed with alternatives for point A, ANS, and PNS: A1, A2, ANS1, ANS2, and PNS1. Patients: One hundred sixty-four children with complete unilateral cleft lip and palate (UCLP), with a lateral head film taken at age 4 to 6 years. Mean Outcome Measures: Intraobserver and interobserver reliability for cephalometric measurements including A, ANS, PNS or their alternatives: Dahlberg errors, systematic errors, and Pearson correlation coefficients were calculated. Results: The measurements using ANS and PNS or their alternatives, were comparable. The systematic error between observers for measurements using A2 was less than for measurements using A or A1. The scatterplot of point A showed a slightly better distribution of the points than the plots of A1 and A2. Conclusions: Although the landmarks A, ANS, and PNS are hard to trace in UCLP patients with tooth germs in the anterior maxilla, no better landmarks were found in this study. Cephalometric studies using A, ANS, and PNS in UCLP patients should be interpreted with caution.
Mandibular Condyle Lesions in Children with Juvenile Idiopathic Arthritis
Authors: Sidiropoulou-Chatzigianni Sossani , Papadopoulos Moschos A. , Kolokithas George 
Published: January 2008

Objective: To assess the prevalence of radiographically detectable destruction of the temporomandibular joints in children with juvenile idiopathic arthritis and to study the possible relationships between condylar destruction and type and duration of the disease, as well as the type of occlusion. Material And Method: The study group consisted of 66 children with juvenile idiopathic arthritis (27 boys, 39 girls; mean age, 11.9 years). The possible presence of condylar destruction was examined in panoramic radiographs. The medical history and the type of malocclusion were registered also. The statistical evaluation was performed by means of descriptive statistics, Student's t test, Pearson's chi-square, and an analysis of variance test. The whole procedure was repeated after a 4-week interval to estimate the error of the method. Results: Of the children with juvenile idiopathic arthritis, 50% showed some form of condylar destruction. Significant correlation was found between the type of the disease and the condyles affected. In the polyarticular type of juvenile idiopathic arthritis, 75% of the children presented affected condyles and 55.6% of them showed lesions bilaterally. The condylar affection was found to be independent of sex, although girls showed a tendency to bilateral lesions. In children with unilateral destruction, the right condyle was affected four times more frequently than the left. The duration of juvenile idiopathic arthritis seems to be significantly correlated to condylar destruction and especially to bilateral destruction. Conclusion: Children with juvenile idiopathic arthritis presented a remarkable prevalence of condylar destruction, which was correlated to the type and duration of the disease.
Canine Eruption in Patients with Complete Cleft Lip and Palate
Authors: Russell Kathleen A. D. D. S. , McLeod Catherine E. 
Published: January 2008

Objective: To describe erupting maxillary canine positions in patients with bone-grafted alveolar clefts. Sample: The sample consisted of 101 cleft sites from patients with complete unilateral or bilateral cleft lip and palate who had early (≤9 years) or late (>9 years) secondary alveolar bone grafts. Methods: Canine position was assessed using panoramic radiographs taken before and after alveolar bone grafts. Vertical canine positions were assessed using the long axis of the maxillary permanent canine relative to a 90° vertical reference line. Lateral canine positions were defined using the relationship between the canine tip and the midplane of the lateral incisor root. Anomalous lateral incisors were recorded. Statistical analysis included Student's t tests and chi-square tests. Results: Patients with alveolar clefts had a 20-fold increased risk for canine impaction, based on erupting canine positions. Abnormal vertical canine positions decreased following early and late alveolar bone grafts ( p < .05), whereas abnormal lateral canine positions increased following late alveolar bone grafts ( p < .01). Of the cleft sites with altered canine positions, 61% also had a lateral incisor anomaly. Based on canine position, the non–cleft-side canine had the same risk for impaction as the cleft-side canine. Conclusions: Patients with alveolar clefts have a significantly higher risk for canine impaction compared with patients without clefts. Timing of alveolar bone grafts and lateral incisor anomalies influenced the risk for canine impaction. An alveolar bone graft should be planned in accordance with maxillofacial development, including the eruption of teeth adjacent to the cleft.
Forced Myocardin Expression Enhances the Therapeutic Effect of Human Mesenchymal Stem Cells After Transplantation in Ischemic Mouse Hearts
Authors: Grauss Robert W. , van Tuyn John , Steendijk Paul , Winter Elizabeth M. , Pijnappels Daniël A. , Hogers Bianca , Gittenberger-De Groot Adriana C. , van der Geest Rob , van der Laarse Arnold , de Vries Antoine A.F. , Schalij Martin J. , Atsma Douwe E. 
Published: January 2008

Abstract
Human mesenchymal stem cells (hMSCs) have only a limited differentiation potential toward cardiomyocytes. Forced expression of the cardiomyogenic transcription factor myocardin may stimulate hMSCs to acquire a cardiomyogenic phenotype, thereby improving their possible therapeutic potential. hMSCs were transduced with green fluorescent protein (GFP) and myocardin (hMSCmyoc) or GFP and empty vector (hMSC). After coronary ligation in immune-compromised NOD/scid mice, hMSCmyoc (n = 10), hMSC (n = 10), or medium only (n = 12) was injected into the infarct area. Sham-operated mice (n = 12) were used to determine baseline characteristics. Left ventricular (LV) volumes and ejection fraction (EF) were serially (days 2 and 14) assessed using 9.4-T magnetic resonance imaging. LV pressure-volume measurements were performed at day 15, followed by histological evaluation. At day 2, no differences in infarct size, LV volumes, or EF were observed among the myocardial infarction groups. At day 14, left ventricular ejection fraction in both cell-treated groups was preserved compared with the nontreated group; in addition, hMSCmyoc injection also reduced LV volumes compared with medium injection (p &lt; .05). Furthermore, pressure-volume measurements revealed a significantly better LV function after hMSCmyoc injection compared with hMSC treatment. Immunohistochemistry at day 15 demonstrated that the engraftment rate was higher in the hMSCmyoc group compared with the hMSC group (p &lt; .05). Furthermore, these cells expressed a number of cardiomyocyte-specific markers not observed in the hMSC group. After myocardial infarction, injection of hMSCmyoc improved LV function and limited LV remodeling, effects not observed after injection of hMSC. Furthermore, forced myocardin expression improved engraftment and induced a cardiomyocyte-like phenotype hMSC differentiation.
Disclosure of potential conflicts of interest is found at the end of this article.
Pluripotency Associated Genes Are Reactivated by Chromatin-Modifying Agents in Neurosphere Cells
Authors: Ruau David , Ensenat-Waser Roberto , Dinger Timo C. , Vallabhapurapu Duttu S. , Rolletschek Alexandra , Hacker Christine , Hieronymus Thomas , Wobus Anna M. , Müller Albrecht M. , Zenke Martin 
Published: January 2008

Abstract
Chromatin architecture in stem cells determines the pattern of gene expression and thereby cell identity and fate. The chromatin-modifying agents trichostatin A (TSA) and 5-Aza-2′-deoxycytidine (AzaC) affect histone acetylation and DNA methylation, respectively, and thereby influence chromatin structure and gene expression. In our previous work, we demonstrated that TSA/AzaC treatment of neurosphere cells induces hematopoietic activity in vivo that is long-term, multilineage, and transplantable. Here, we have analyzed the TSA/AzaC-induced changes in gene expression by global gene expression profiling. TSA/AzaC caused both up- and downregulation of genes, without increasing the total number of expressed genes. Chromosome analysis showed no hot spot of TSA/AzaC impact on a particular chromosome or chromosomal region. Hierarchical cluster analysis revealed common gene expression patterns among neurosphere cells treated with TSA/AzaC, embryonic stem (ES) cells, and hematopoietic stem cells. Furthermore, our analysis identified several stem cell genes and pluripotency-associated genes that are induced by TSA/AzaC in neurosphere cells, including Cd34, Cd133, Oct4, Nanog, Klf4, Bex1, and the Dppa family members Dppa2, 3, 4, and 5. Sox2 and c-Myc are constitutively expressed in neurosphere cells. We propose a model in which TSA/AzaC, by removal of epigenetic inhibition, induces the reactivation of several stem cell and pluripotency-associated genes, and their coordinate expression enlarges the differentiation potential of somatic precursor cells.
Disclosure of potential conflicts of interest is found at the end of this article.