bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incorporating findings from a vaccine refusal study to develop a community play to improve knowledge on maternal vaccines in rural Kilifi, Kenya
Authors: Kiyuka Patience Kerubo , Onsomu Rodgers , Mutua Meshack Nzesei , Mumba Noni , Shauri Halimu 
Published: March 2024

Abstract
Background: Innovative approaches are urgently needed to address the growing concern of vaccine hesitancy. Used effectively, community theatre has the potential to promote health knowledge and behaviour change as it encourages community dialogues and information sharing.
Methods: This project was conducted between May 2020 and Feb 2021. We used research findings from a vaccine hesitancy study to develop a community play on maternal vaccines. The development of the script for the play was an iterative process between a local community theatre group and the research team.
Results: After taking into consideration feedback from the relevant stakeholders, we produced a community play that sought to address concerns around maternal vaccines. The resulting play was entertaining and culturally relevant and conveyed the message in a simple and clear manner using the local language.
Conclusions: Research-based theatre provides innovative ways to improve public understanding of maternal vaccines. Researchers in similar settings may find our drama relevant and adaptable.
Catalysis Properties of TiO2 Nanofibers in CO2 Conversion: A Comparative Analysis of Polymer Matrices
Authors: Gehlot Karan , Kothari Anil Chandra , Tiwari Sangeeta , Bal Rajaram , Tiwari Sandeep Kumar 
Published: March 2024

Abstract
The quest for efficient and sustainable methods to mitigate Carbon Dioxide (CO2) emissions is a pressing global challenge. This study delves into the crucial role of polymers in tailoring the performance of Titanium Dioxide (TiO2) nanofibers for CO2 conversion reactions. By systematically comparing the influence of different polymers, specifically Polyvinyl Pyrrolidone (PVP) and Polyvinylidene Fluoride (PVDF), on the CO2 conversion activity of TiO2-NFs, we shed light on the remarkable potential of polymeric selection to fine-tune catalyst properties. The paper uses advanced experimental techniques to analyze the structural and morphological properties of PVP-TiO2-NFs and PVDF- TiO2-NFs demonstrating their various morphologies. The investigation involves SEM, TEM, XRD, BET and UV-Vis spectroscopy to better understand the charge separation and recombination processes involved in both materials' CO2 conversion. The results show considerable differences, choice of polymer significantly impacts the CO2 conversion performance of TiO2-NFs. PVP based NFs exhibit enhanced surface area and porosity, resulting in superior catalytic activity, while PVDF based NFs demonstrate remarkable stability. These findings pave the way for innovative approaches to tackle climate change and develop a more environmentally friendly future by advancing energy-efficient and long-lasting photocatalytic technology.
Optimization of SBR Biological Treatment System and Advanced Oxidation for Non-Biodegradable Gray Water Treatment
Authors: Govahi Saeid , Derikvand Ehsan , Khoshnavaz Saeb , Babarsad Mohsen Solimani , Parseh Iman 
Published: March 2024

Abstract
This study investigates the treatment of gray water with high detergent content through a combination system of sequential batch reactor (SBR) and UV/H2O2 chemical oxidation. Preliminary analysis showed COD, BOD, TP and TKN levels of 600, 200, 4 and 15 mg/L respectively. As a result of SBR treatment, BOD5 decreased from 270 to 40 mg/L and COD from 600 to 162 mg/L. Activated carbon powder showed the least effect on pollutant removal efficiency. The optimal concentration of H2O2 was 12 mg/L and the duration of 3 hours for chemical oxidation was determined. The duration of aerobic and anaerobic SBR more than 4 and 2 hours, respectively, had negligible effects on pollutant removal. The combined SBR and chemical oxidation system achieved removal efficiencies of 90.6, 85, 82.5, and 65% for COD, BOD, TP, and TKN, respectively. Placing chemical oxidation before biological treatment increased organic compound removal by 6% because chemical oxidation increased biodegradable compounds. In addition, using an anaerobic unit to remove phosphorus before aeration was effective, more than two hours of anaerobic treatment had no effect on the removal of dissolved phosphorus.
Cervical cancer lymphovascular infiltration determination and tumor-associated macrophage enhancing immune escape microenvironment analysis
Authors: Guan Liming , Xu Xuexiang , Xu Junhao , Xu Gang , Zhang Yunzhu , Xia Haitao 
Published: March 2024

Abstract

Background:Lymphovascular infiltration(LVI) reduced cervical cancer patients overall survival(OS),while it was not easily discovered by routine HE staining,while the role of tumor-associated macrophages(TAMs) in this course need define.
Methods: Early stage cervical cancer patients were received carbon nanoparticles(CNP) for sentinel lymph nodes(SLNs) mapping,laparotomy pelvic lymph node dissection and radical hysterectomy.The samples were detected for ultra staging,cocktail double immunohistochemical(IHC) staining,Western blot anlysis.Single cell data from(GEO) for cervical cancer were obtained and analysis.
Results: The combine of CNP mapping,ultra staging and double IHC staining enhance determining ratio for tumor LVI than that of HE staining alone(41.8% (41/98) vs. 20.4% (20/98), P=0.046)).
When the number of vascular invasion foci>2.0,or the number of cancer emboli cells ≥5.0, there was negative correlation with the OS for patients (p < 0.05).
More M2 macrophage emerged surrounding the tumor vasculature than surroungding normal cervix(P<0.05).Western blot analysis,TAMs related genes MMP2,SPARC and GNLY expessed higher level in tumor, while the OS of the patients decreased accordingly.
Single cell data were screened, reduced dimensions, and constructed pseudo-trajectories. Differentially expressed genes (DEGs) analysis showed, M2 macrophage clusters related genes expession level MMP2,SPARC and GNLY were obviosely higher than that of M1 clusters in cervical cancer; M1/M2 ratio decreased significantly. MMP2,GNLY could destroy tumor vasculauture basement membrane; SPARC anti-adhesion, played a key role in EMT and tumor invasion.
M1 macrophages cluster anti-inflammatory, immune surveillance and anti-immune escape scores were obviously higher than that of M2 macrophages cluster.
Gene Ontology(GO) analysis showed innate immune response gene expression for M2 macrophage clusters decreased obviously than that of M1 macrophages.Kyoto Encyclopedia of Genes and Genomes(KEGG) showed that normal cervix and precancerous lesions higher enrichement of M1 macrophage clusters, presenting positive regulation of response stimulus signaling pathway,while cervical cancer and metastatic lymph nodes enriched M2 clusters presenting negative regulation to these signaling pathway.
Conclusions:CNP mapping could be as a SLN tracer in early cervical cancer LVI screening.Cocktail double IHC staining could enhance diagnostic efficiency than HE staining in determining LVI. M2 TAMs activity increased from precancerous lesions to cervical cancer forming a immunosuppressive envioment gradually, TAMs related genes could play a key role in this process.
Association between Sex Hormones and Erectile Dysfunction in Men without Hypogonadism
Authors: Fujita Naoki , Okamoto Teppei , Yamamoto Hayato , Yoneyama Takahiro , Hashimoto Yasuhiro , Mikami Tatsuya , Itoh Ken , Ohyama Chikara , Hatakeyama Shingo 
Published: March 2024

Abstract
In addition to testosterone, various endocrine hormones, such as dehydroepiandrosterone sulfate (DHEA-S) and estradiol, may be involved in erectile function. However, the role of these sex hormones in the erectile function of men without hypogonadism remains unclear. This cross-sectional study included 398 community-dwelling men without hypogonadism. The participants were categorized into the non-ED and ED groups. Multivariable logistic regression analyses were performed to investigate the relationship between ED and serum sex hormone levels, including total testosterone, DHEA-S, estradiol, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and prolactin. Among the 398 men, 66 (17%) and 332 (83%) were categorized into the non-ED and ED groups, respectively. In the multivariable analyses, serum DHEA-S and estradiol levels were significantly associated with ED (odds ratio [OR]: 0.996, P = 0.030; OR: 1.082, P = 0.002; respectively), whereas serum total testosterone, LH, FSH, and prolactin levels did not demonstrate significant association. After adjusting for age, none of neutrophil-to-lymphocyte ratio, serum plasminogen activator inhibitor-1 levels, and skin advanced glycation end-products levels demonstrated significant correlation with serum DHEA-S and estradiol levels. In conclusion, lower testosterone levels did not affect ED in men with normal testosterone levels, whereas serum DHEA-S and estradiol levels were significantly associated with ED.
Concentration and Geospatial Modelling of Health Development Offices' Accessibility for the Total and Elderly Populations in Hungary
Authors: Domjan Peter , Angyal Viola , Vingender Istvan 
Published: March 2024

Abstract
Background
This study examines the availability and national distribution of Health Development Offices (HDOs; N = 108) in Hungary, with an emphasis on their role in health prevention for the general and elderly population. HDOs play a crucial role in providing preventive services (nutrition, physical activity, mental hygiene), a significant factor in the health preservation of the elderly. The geographical location and accessibility of these Offices are essential parameters as they influence individual participation willingness.
Methods
Leveraging advanced geospatial modelling techniques with QGIS 3.34.0 and MS Excel software, we mapped the locations of HDOs relative to population centres, employing statistical tools such as the Lorenz curve and Gini index, LQ index, and Herfindahl-Hirschman Index. These methods allowed for a nuanced analysis of service concentration and the identification of geographic disparities in service provision. The stochastic relationship between the population and the number of HDOs was analysed through linear regression. This spatial and demographic study was based on 2022 data.
Results
The number of HDOs did not indicate significant spatial concentration relative to the population, although the Entropy Index measured substantial diversity among the counties. Based on the measured LQ Index values, it can be stated that the presence of HDOs is underrepresented in the capital and its surroundings, as well as in several counties. Additionally, our regression analysis indicated that an increase in population size does not necessarily equate to an increase in the number of HDOs.
Conclusion
The examination of geocoordinates through scatter plots, indicated a broad spectrum of dispersion, and the placement of HDOs on the map revealed a star topology. From the findings of our research, it can be concluded that the Hungarian network of Health Development Offices (N = 108) can meet the preventive health needs of both the general and the elderly population. Enhancing the geographical spread of HDOs is crucial for improving the accessibility and effectiveness of health prevention strategies, especially among Hungary's aging population, thereby contributing to a more equitable health service landscape.
A novel integrated interval prediction method based on small and non-normality distributed datasets for pavement performance from the uncertainty perspective
Authors: Zuo Wei 
Published: March 2024

Abstract

The uncertainty prediction of pavement performance can promote intelligent highway tunnel operation and maintenance, but it encounters the challenges of small and non-normality distributed datasets. This paper proposes a novel integrated interval prediction method to overcome these shortcomings so that we can decrease the uncertainty. This paper also validates the effectiveness of the proposed method using the empirical test of pavement performance data from the Dalian Road Tunnel in Shanghai, China. The evaluated results of PINAW and PICP achieve the values of 0.2262 and 89.24%, respectively, demonstrating excellent uncertainty prediction. Furthermore, this paper applied the proposed method to other datasets, which exhibit good generalization ability and thereby be beneficial for formulating scientific maintenance decisions and achieving the maximum service benefits of road surfaces. At last, our code will be open-sourced.
Antibacterial, Antioxidant and Anticancer Activities of the Streptomyces PML5 strain isolated from Carbonate rocksin the Amazon
Authors: Chaves Karine Rodrigues do Nascimento , França Maria Lucila Texeira de Andrade , Mendes Anna Ludmylla Oliveira , Cardoso Pablício Pereira , Vasconcelos Kamila Brielle Pantoja , de Santana Raphael Carlos Ferrer , Lotufo Letícia Veras Costa , Ramos Sara Freitas de Sousa , Costa Isabella Cristhina Gonçalves , Mendes Anderson Conceição , Ferreira Lindalva Maria de Meneses Costa , Ferreira Marcelo J. Pena Ferreira J. Pena , Padilla Gabriel , Silva Silvia Katrine Rabelo da 
Published: March 2024

Abstract
The aim of this study was to investigate the antimicrobial and cytotoxic potential of Streptomyces sp. isolated from carbonate rock in the Amazon. The antimicrobial activity was verified by the diffusion technique in agar against pathogens such as Micrococcus lutteus ATCC 7468, Streptococcus pneumoniae ATCC700699; Proteus vulgaris ATCC 13315, Escherichia coli ATCC 10536, Acinetobacter baumanii ATCC 19606.Cytotoxic activity was investigated ​​by the inhibition of cell growth (%) of samples tested in human colorectal carcinoma cell line (HCT116), at a concentration of 10 µg/mL (mean ± SEM; n = 2). The isolate showed a broad spectrum of antimicrobial activity against test microorganisms. This isolate showed maximum antibacterial activity against Escherichia coli ATCC 10536 (13 ± 0.0 mm) and Proteus vulgaris ATCC 13315 (12.8 ± 0.29 mm) and the most resistant microorganism against antibacterial activity of this isolate was Acinetobacter baumanii ATCC 19606 (11.5 ± 1.15 mm). This metabolites was extracted by solid:liquid method with ethyl acetate organic solvent. ​​The percentage of inhibition of cell growth of the crude extract tested in a human colorectal carcinoma line (HCT116) was 96,55%. The determination of antioxidant activity by capturing the free radical ABTS had an inhibition of 35.60 ± 2.01%, while the antioxidant potential by capturing the free radical DPPH had an inhibition of 10.65 ± 1.95%. The LC-MS analysis showed the presence of four peaks, where it was identified that the absorbance of substances 5,791 and 13,556 were similar to 9,921 and 17,077, respectively; this suggests the possibility of PML 5 having a metabolic pathway synthesizing substances of the same class. The CG-MS of the extract showed the presence of 10 volatile compounds. The main compound was Cyclododecane (83.75%) compared to other bioactive compounds. These results reinforce the potential of Amazonian biodiversity, revealing that actinobacteria produce antimicrobial and cytotoxic bioactive substances of interest to the pharmaceutical industry.
Multivariate Shape analysis using modified Otsu’s method for High-Resolution Remote-Sensing Image Segmentation
Authors: R Shijitha , A Stanly Paul 
Published: March 2024

Abstract

The image segmentation is mentioned to as a vital practices of image processing. It is the procedure of separating or apportioning an image into portions, named segments. It is typically suitable for applications alike image compression or object identification, since for them, it is incompetent to practice the entire image. Therefore, image segmentation is employed to divide the portions from an image for additional handling. Various image segmentation methods are availed, which divide the image into numerous portions according with definite image topographies such as pixel intensity, color, texture, etc. In this study, the protuberant approaches such as local homogeneity analysis, openings and closings with reconstruction criteria and modified Otsu’s method have been utilized for image segmentation. While comparing the obtained results of local homogeneity analysis and openings and closings with reconstruction criteria, the modified Otsu’s method offers adequate outcomes for the given images with pertinent histograms. The simulation studies reveal that the modified Otsu’s method has the benefits of real time and assured anti-noise skills, the contaminant can be found accurately and made the segmentation process ease and consistent.
Evaluation of the impact of COVID-19 on hepatitis B in Henan Province and its epidemic trend based on Bayesian structured time series model
Authors: Li Xinxiao , Li Yanyan , Xu Shushuo , Wang Penghao , Hu Meng , Li Haibin 
Published: March 2024

Abstract
Background
There may be evidence that COVID-19 affects illness patterns. This study aimed to estimate epidemiological trends in China and to assess the effects of COVID-19 epidemic on the declines in hepatitis B (HB) case notifications.
Methods
The Bayesian structured time series (BSTS) method was used to investigate the causal effect of COVID-19 on the decline in HB cases based on the monthly incidence of HB from January 2013 to September 2022. To assess how well the BSTS algorithm performs predictions, we split the observations into various training and testing ranges.
Results
The incidence of HB in Henan was generally declining with periodicity and seasonality. The seasonal index in September and February was the smallest (0.91 and 0.93), and that in March was the largest (1.19). Due to the COVID-19 pandemic, the monthly average number of notifications of HB cases decreased by 38% (95% credible intervals [CI]: -44% ~ -31%) from January to March 2020, by 24% (95% CI: -29% ~ -17%) from January to June 2020, by 15% (95% CI: -19% ~ -9.2%) from January to December 2020, by 11% (95% CI: -15% ~ -6.7%) from January 2020 to June 2021, and by 11% (95% CI: -15% ~ -7.3%) from January 2020 to December 2021. From January 2020 to September 2022, it decreased by 12% (95% CI: -16% ~ -8.1%). From 2021 to 2022, the impact of COVID-19 on HB was attenuated. In both training and test sets, the average absolute percentage error (10.03%) generated by the BSTS model was smaller than that generated by the ARIMA model (14.4%). It was also found that the average absolute error, root mean square error, and root mean square percentage error generated by the BSTS model were smaller than ones generated by the ARIMA model. The trend of HB cases in Henan from October 2022 to December 2023 predicted by the BSTS model remained stable, with a total number of 81,650 cases (95% CI: 47,372 ~ 115,391).
Conclusions
After COVID-19 intervention, the incidence of HB in Henan decreased and exhibited clear seasonal and cyclical trends. The BSTS model outperformed the ARIMA model in predicting the HB incidence trend in Henan. This information may serve as a reference and provide technical assistance for developing strategies and actions to prevent and control HB. Take additional measures to accelerate the progress of eliminating HB.
Extraction and characterization of cellulose nanocrystals from brown seaweed Dictyota bartayreisana, J.V.Lamouroux
Authors: Murugesan Sobiya , R Radhika Rajasree S , Rajan Roopa 
Published: March 2024

Abstract
For the first time, cellulose nanocrystal (CNC) was derived from the biomass of brown seaweed Dictyota bartayresiana, undergoing a comprehensive process of extraction and transformation. The structural analysis, conducted via Transmission Electron Microscopy (TEM), affirmed that the resulting CNCs displayed an average width of approximately 26 nm and a length extending to 520nm long. X-ray Diffraction (XRD) analysis indicated that these extracted CNCs constituted around 62%. Fourier Transform Infrared (FTIR) spectral analysis confirmed the successive removal of non-cellulosic components through chemical treatments. Elemental analysis (CHNS) validated the presence of sulphate groups, accounting for 0.59%. Thermogravimetric Analysis (TGA) results unveiled the superior thermal stability of the extracted CNCs.
Effective Removal of Ni(II)ions from Aqueous Solutions Using Low-Cost, Eco-Friendly Natural and Modified Potato Peels
Authors: BULUT Aycan , Altunkaynak Yalçın 
Published: March 2024

Abstract
The objective of this investigation is to look into the potential use of trash potato peels (PP) and processed potato peels (MPP) as a solution for effectively removing Ni(II) ions extracted from aqueous solutions. The optimal working circumstances for removing Ni(II) ions from aqueous solution in PP are an initial concentration of 500 mg/L, an adsorbent dose of 0.3 g, a contact time (CT) of 100 minutes, and a solution pH of 6.21. The optimal working conditions for MPP were established to be an initial concentration of 500 mg/L, an adsorbent dosage of 0.15 g, a CT of 100 minutes, and a solution pH of 6.21. Studies were conducted at various temperatures to better understand the removal capabilities of adsorbent compounds. The removal abilities of Ni(II) ions for PP were determined to be 25.125, 29.325, and 33.112 mg/g at varied temperatures (25, 35, and 45 oC), respectively. Under the same conditions, the removal capacity of MPP was determined to be 68.965, 78.125, and 85.470 mg/g. Further investigation of the adsorption kinetics revealed that the experimental results suited the pseudo-second-order model for both PP and MPP adsorbents. Thermodynamic analyses demonstrated that the endothermic process of Ni (II) metal ion adsorption onto the adsorbents occurred spontaneously. The study found that both PP and MPP were very efficient and ecologically friendly adsorbents for removing Ni (II) ions from aqueous solutions. Their demonstrated performance highlights their potential value in sustainable wastewater treatment applications, which accord with environmentally conscious behaviors. Their high removal capacity to effectively remove metal ions, together with their ease of use, low cost, and origin as agricultural trash, make them potential adsorbents for long-term uses in both water purification and environmental cleanup.
Directions and prospects for the development of health resort tourism in Poland
Authors: Soroka Andrzej , Godlewska Agnieszka 
Published: March 2024

Abstract
Background: The objective of the study was to evaluate the current level of the functioning of health resort tourism, and identify factors which exert the greatest influence on this type of tourism. The authors attempted to determine the tasks and directions of the development of health resort tourism, and define factors which may have the greatest positive impact on the standards they provide.
Materials and methods. Of the 258 health resorts, 154 establishments were selected for research purposes taking into account a proportional distribution of health resorts throughout Poland. An authorial questionnaire was developed and it included five questions with multiple answers, each answer being assessed on a 5-point Likert scale.
Results. Younger people expect a high standard of holiday resort infrastructure and better conditions for disabled people. The older the respondents are, the more likely they are to seek diverse forms of active relaxation which affect the physical and mental condition as well as regeneration of the body.
Conclusions. The awareness of the Polish society that healthy lifestyle is a necessity is increasing and is related to the age of treatment-seekers, their state of health and needs, which confirms the validity of the study reported here. The segmentation of customer profile obtained in the study reported here and individual monitoring ought to be systematic as this is the only way to evaluate the functioning, level and needs of visitors, and strengthen the potential of health resorts.