bluuebunny/crossref_metadata_2025_split huggingface dataset:
An independent origin of an annual life cycle in a North American killifish species
Authors: Domínguez-Castanedo Omar , Thompson Andrew W , Meredith Robert W , Furness Andrew I 
Published: March 2024

Abstract
An annual life cycle is characterized by short-lived adults that exploit seasonally productive but temporary habitats, paired with a hardy embryo stage that survives unfavourable environmental conditions, such as winter or the dry season. This life cycle is rare amongst vertebrates but has evolved independently several times in African and South American killifishes adapted to life in seasonally ephemeral aquatic pools. A single species of North American annual killifish, with a limited geographical distribution in Mexico, also exhibits this seasonal life cycle. This enigmatic species, Millerichthys robustus, is geographically separated from the nearest South American annual killifish species by &gt;2000 km. Millerichthys robustus exhibits morphological features that have led to several competing phylogenetic hypotheses. To date, its phylogenetic relationships are unclear because no molecular data have been available. Here, we sequence the mitochondrial genome of Millerichthys robustus and generate a molecular phylogeny of killifishes that includes this species. Our results indicate that, rather than being most closely related to South American annual killifishes, this species is sister to two non-annual killifish species from Cuba (Rivulus cylindraceus and Rivulus berovidesi). Ancestral state reconstruction strongly supports an independent origin of an annual life cycle and embryonic diapause in Millerichthys robustus.
William Thompson and John Stuart Mill on co-operation and the rights of women
Authors: Prendergast Renee 
Published: March 2024

Abstract
William Thompson and, later, John Stuart Mill argued that women’s inferior position in society was a product of their environment and upbringing. As such, access to the franchise, and opportunities for education and employment would improve the welfare and position of women. Recognition of women’s reproductive roles led Thompson to argue that equality of outcome for women could not be achieved within the competitive framework and required a re-organisation of society into self-supporting co-operative communities in which women’s reproductive role would be valued and childcare and catering provided communally. While John Stuart Mill advocated access for women into all employments, he thought that a purely domestic role was consistent with women’s emancipation provided that role was freely chosen. Free choice was supported by better outside options but the bargaining power of women choosing the domestic route was not addressed by Mill. Mill supported co-operation but his favoured form involved co-operative ownership of firms operating within competitive markets. He believed that a society based on co-operation would foster favourable attitudes towards women’s emancipation and moral improvement more generally, but he saw no direct relationship between the development of co-operative enterprise and the status of women.
Brushing aside doubts: an evaluation of the beat-sheet brushing technique for detecting the Nearctic tree trunk sheetweaver (Araneae: Linyphiidae)
Authors: Forsythe Patrick S , Saxby Justice A , Fritsch Haillee R , Hoffmann Noah P , Ditzman Teona T , Suess Deakyn J , Radobicky Kelsey A , Schmitz Bennett A , Amasone Samantha D , Buchmann Charles K , Schultz Laken T , Clauer Addison L , Vista Allison S , Kienbaum April M , Ryan-Rabe Megan R , Monfils Brooklyn A M , Lara Bryan A , Draney Michael L 
Published: March 2024

Abstract
It is important to have reliable information on the presence/absence, population structure, and density of animals across their natural range. Detecting small organisms, however, such as the Nearctic tree trunk sheetweaver spider Drapetisca alteranda Chamberlin 1909 (Araneae: Linyphiidae), presents challenges due to its diminutive size and cryptic nature. We used a capture/recapture study to determine the detection and recapture probabilities of this spider using a standard beat sheet technique adopted for surveying tree trunks. Spiders were released on 3 different tree species that provided a range of microhabitats, including variable bark surface area and furrow depth/width. Microhabitat features played a small role in the timing of spider recapture (i.e., slower rate of recapture as furrowing increased). However, our results demonstrated 100% detection across replicate experiments and individual recapture probabilities exceeding 90% in most situations, with no significant differences in recapture observed among tree species and with respect to tree circumference. Furthermore, we show that most spiders could be recaptured within 2 sampling revolutions around the tree trunk, and there was no difference in the probability of collecting male and female spiders (although they differ markedly in size). Finally, we found no difference among brushers, supporting the idea that this method is replicable across collectors and studies. Collectively, we establish confidence in the ecological knowledge obtained with this technique and encourage its application with similar species and systems.
Top-Category Inflation in Ordered International Relations Outcomes
Authors: Bagozzi Benjamin E , Joo Minnie M , Mukherjee Bumba 
Published: March 2024

Abstract
Ordered dependent variables are widely employed in international relations (IR). These ordered dependent variables often suffer from inflated observations in their highest outcome category due to distinct processes. The application of standard ordered probit and ordered logit models to such ordinal measures will fail to capture these distinct processes, often producing biased inferences as a result. Yet IR researchers have thus far ignored the potential for top-category inflation in ordered outcome variables. We sensitize researchers to top-category inflation in ordered IR outcomes. We then intuitively extend the widely used zero-inflated ordered probit model to the top-category inflated setting, providing resources to facilitate the proper modeling of top-category inflation in ordered outcomes. Finally, we provide two applications to published IR research related to trade politics and political repression. Together, these applications illustrate the substantive and methodological potentials of our proposed tools for diagnosing and modeling top-category inflation in IR outcomes.
Characterization of the Pristionchus pacificus “epigenetic toolkit” reveals the evolutionary loss of the histone methyltransferase complex PRC2
Authors: Brown Audrey L , Meiborg Adriaan B , Franz-Wachtel Mirita , Macek Boris , Gordon Spencer , Rog Ofer , Weadick Cameron J , Werner Michael S 
Published: March 2024

Abstract
Comparative approaches have revealed both divergent and convergent paths to achieving shared developmental outcomes. Thus, only through assembling multiple case studies can we understand biological principles. Yet, despite appreciating the conservation—or lack thereof—of developmental networks, the conservation of epigenetic mechanisms regulating these networks is poorly understood. The nematode Pristionchus pacificus has emerged as a model system of plasticity and epigenetic regulation as it exhibits a bacterivorous or omnivorous morph depending on its environment. Here, we determined the “epigenetic toolkit” available to P. pacificus as a resource for future functional work on plasticity, and as a comparison with Caenorhabditis elegans to investigate the conservation of epigenetic mechanisms. Broadly, we observed a similar cast of genes with putative epigenetic function between C. elegans and P. pacificus. However, we also found striking differences. Most notably, the histone methyltransferase complex PRC2 appears to be missing in P. pacificus. We described the deletion/pseudogenization of the PRC2 genes mes-2 and mes-6 and concluded that both were lost in the last common ancestor of P. pacificus and a related species P. arcanus. Interestingly, we observed the enzymatic product of PRC2 (H3K27me3) by mass spectrometry and immunofluorescence, suggesting that a currently unknown methyltransferase has been co-opted for heterochromatin silencing. Altogether, we have provided an inventory of epigenetic genes in P. pacificus to compare with C. elegans. This inventory will enable reverse-genetic experiments related to plasticity and has revealed the first loss of PRC2 in a multicellular organism.
Expanding TheCellVision.org: a central repository for visualizing and mining high-content cell imaging projects
Authors: Masinas Myra Paz David , Litsios Athanasios , Razdaibiedina Anastasia , Usaj Matej , Boone Charles , Andrews Brenda J 
Published: March 2024

Abstract
We previously constructed TheCellVision.org, a central repository for visualizing and mining data from yeast high-content imaging projects. At its inception, TheCellVision.org housed two high-content screening (HCS) projects providing genome-scale protein abundance and localization information for the budding yeast Saccharomyces cerevisiae, as well as a comprehensive analysis of the morphology of its endocytic compartments upon systematic genetic perturbation of each yeast gene. Here, we report on the expansion of TheCellVision.org by the addition of two new HCS projects and the incorporation of new global functionalities. Specifically, TheCellVision.org now hosts images from the Cell Cycle Omics project, which describes genome-scale cell cycle-resolved dynamics in protein localization, protein concentration, gene expression, and translational efficiency in budding yeast. Moreover, it hosts PIFiA, a computational tool for image-based predictions of protein functional annotations. Across all its projects, TheCellVision.org now houses &gt;800,000 microscopy images along with computational tools for exploring both the images and their associated datasets. Together with the newly added global functionalities, which include the ability to query genes in any of the hosted projects using either yeast or human gene names, TheCellVision.org provides an expanding resource for single-cell eukaryotic biology.
Exercise to Mitigate Cerebrovascular Aging: A Geroscience Perspective
Authors: Norling Amani M , Lipsitz Lewis A 
Published: March 2024

Abstract
Aging is characterized by a progressive loss of cellular functions that increase the risk of developing chronic diseases, vascular dysfunction, and neurodegenerative conditions. The field of geroscience has identified cellular and molecular hallmarks of aging that may serve as targets for future interventions to reduce the risk of age-related disease and disability. These hallmarks include genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. Several studies show that exercise may favorably affect these processes and thereby have antiaging properties. The primary mechanisms through which exercise confers protective benefits in the brain are still incompletely understood. To better understand these effects and leverage them to help promote brain health, we present current findings supporting the notion that adaptive responses to exercise play a pivotal role in mitigating the hallmarks of aging and their effects on the aging cerebrovasculature, and ultimately contribute to the maintenance of brain function across the healthspan.
Quantifying the role of submesoscale Lagrangian transport features in the concentration of phytoplankton in a coastal system
Authors: Veatch Jacquelyn M , Kohut Josh T , Oliver Matthew J , Statscewich Hank , Fredj Erick 
Published: March 2024

Abstract
Food resources in the ocean are often found in low densities, and need to be concentrated for efficient consumption. This is done in part by oceanographic features transporting and locally concentrating plankton, creating a highly patchy resource. Lagrangian approaches applied to ocean dynamics can identify these transport features, linking Lagrangian transport and spatial ecology. However, little is known about how Lagrangian approaches perform in ageostrophic coastal flows. This study evaluates two Lagrangian Coherent Structure metrics against the distribution of phytoplankton; Finite Time Lyapunov Exponents (FTLE) and Relative Particle Density (RPD). FTLE and RPD are applied to High Frequency Radar (HFR) observed surface currents within a biological hotspot, Palmer Deep Canyon Antarctica. FTLE and RPD identify different transport patterns, with RPD mapping single particle trajectories and FTLE tracking relative motion of paired particles. Simultaneous measurements of circulation and phytoplankton were gathered through the integration of vessel and autonomous glider surveys within the HFR footprint. Results show FTLE better defined phytoplankton patches compared to RPD, with the strongest associations occurring in stratified conditions, suggesting that phytoplankton congregate along FTLE ridges in coastal flows. This quantified relationship between circulation and phytoplankton patches emphasizes the role of transport in the maintenance of coastal food webs.
(Political) political liberalism and the constitutional project
Authors: Kuo Ming-Sung 
Published: March 2024

Abstract
This review essay follows Alessandro Ferrara and Frank Michelman’s dialogic journey into the legitimation-by-constitution idea, which they submit as the institutional expression of political liberalism in Legitimation by Constitution: A Dialogue on Political Liberalism, to illustrate the challenges for contemporary philosophy of political liberalism in its transposition to the constitutional project. It argues that Ferrara and Michelman’s liberal endeavor to answer the fundamental questions surrounding political liberalism rests on a normative conception of identity, which eventually leads them to a dilemma: the project of constitutional democracy they defend and justify is sustained by a liberal ethos, while it is tasked to accommodate those who do not value the liberal form of life. Instead of choosing between the project of constitutional democracy and their liberal soul, Ferrara and Michelman eventually offer a thin constitutional account of political liberalism resembling the international legal order amidst the retrenchment of the liberal ethos. It contends that what liberal democracies need from political liberalism in battling antiliberal forces is not more political philosophy but rather something akin to what Jeremy Waldron calls “political political theory.” It is suggested that political liberalism take a political turn to confront antiliberal forces with an enriched rather than diluted constitutional project.
Metabolic syndrome burden, determinants and treatment status in an urban slum resettlement colony in Delhi, India
Authors: Rao Shivani , Basu Saurav , Nandi Kajal , Singh M M , Lalwani Heena , Maheshwari Vansh , Borle Amod , Sharma Nandini 
Published: March 2024

Abstract

Background
Metabolic syndrome (MetS) in low-resource settings contributes to accentuated risk of cardiovascular disease, including stroke. The study objective was to estimate the prevalence, determinants and treatment status of MetS in an urban slum resettlement population in Delhi, India.

Methods
This study was conducted from February to May 2023. Multiphase sampling was conducted with 1910 individuals screened for abdominal obesity (AO), with 996 detected as having AO, of which, 400 were selected by simple random sampling and further evaluated for triglycerides (TGs), high-density lipoprotein (HDL) and fasting glucose levels.

Results
Among the 400 participants detected as having AO, 211 had evidence of MetS (52.75% [95% confidence interval 47.83 to 57.62]). The most prevalent combination of MetS clustering was for all five components (AO, diabetes mellitus [DM], hypertension [HTN], low HDL and high TGs; 14.69%), followed by AO, DM and HTN (12.32%). On adjusted analysis, the odds of having MetS was found to be independently associated with increasing age (≥40 y) but not sex.

Conclusions
A high burden of MetS and suboptimal treatment status is prevalent in urban slum populations. Screening of individuals with AO, especially in those &gt;40 y of age, can be an effective programmatic strategy for early diagnosis and management of MetS and its underlying components.
Imipenem/relebactam pharmacokinetics in critically ill patients supported on extracorporeal membrane oxygenation
Authors: Fratoni Andrew J , Kois Abigail K , Gluck Jason A , Nicolau David P , Kuti Joseph L 
Published: March 2024

Abstract

Background
Extracorporeal membrane oxygenation (ECMO) is a life-saving modality but has the potential to alter the pharmacokinetics (PK) of antimicrobials. Imipenem/cilastatin/relebactam is an antibiotic with utility in treating certain multi-drug resistant Gram-negative infections. Herein, we describe the population pharmacokinetics of imipenem and relebactam in critically ill patients supported on ECMO.

Methods
Patients with infection supported on ECMO received 4–6 doses of imipenem/cilastatin/relebactam per current prescribing information based on estimated creatinine clearance. Blood samples were collected following the final dose of the antibiotic. Concentrations were determined via LC–MS/MS. Population PK models were fit with and without covariates using Pmetrics. Monte Carlo simulations of 1000 patients assessed joint PTA of fAUC0–24/MIC ≥ 8 for relebactam, and ≥40% fT &gt; MIC for imipenem for each approved dosing regimen.

Results
Seven patients supported on ECMO were included in PK analyses. A two-compartment model with creatinine clearance as a covariate on clearance for both imipenem and relebactam fitted the data best. The mean ± standard deviation parameters were: CL0, 15.21 ± 6.52 L/h; Vc, 10.13 ± 2.26 L; K12, 2.45 ± 1.16 h−1 and K21, 1.76 ± 0.49 h−1 for imipenem, and 6.95 ± 1.34 L/h, 9.81 ± 2.69 L, 2.43 ± 1.13 h−1 and 1.52 ± 0.67 h−1 for relebactam. Simulating each approved dose of imipenem/cilastatin/relebactam according to creatinine clearance yielded PTAs of ≥90% up to an MIC of 2 mg/L.

Conclusions
Imipenem/cilastatin/relebactam dosed according to package insert in patients supported on ECMO is predicted to achieve exposures sufficient to treat susceptible Gram-negative isolates, including Pseudomonas aeruginosa.
Experimental investigation of thawing behavior of saline soils using resistivity method
Authors: Chen Cihai , Yang Zhilong , Deng Yaping , Ma Haichun , Qian Jiazhong 
Published: March 2024

Abstract
Electrical resistivity method has been widely used to study permafrost and to monitor the process of freezing-thawing. However, a thorough understanding of the mechanism of electrical response during thawing is missing. In this study, we investigated the thawing behavior of saline soils in the temperature range from roughly −10 to 15°C considering the effects of soil type and salinity. A total of nine experiments were performed with three soil types (silica sand, sandy soil, and silt) and three salinities (0.01, 0.1, and 1 S m−1). The results show that resistivity variations with temperature can be divided into three stages. In Stage I, tortuosity and unfrozen water content play major roles in the decrease of resistivity. In Stage Ⅱ, which is an isothermal or near isothermal process, resistivity still decreases slightly due to the thawing of residual ice and pore water movement. In Stage III, ionic mobility plays an important impact on decreasing resistivity. In addition, the isothermal process is found to only occur in silica sand that can be explained by latent heat effect. Exponential and linear models linking temperature with resistivity are used to fit the experimental data in Stages I and III. The fitting parameter in different models shows great correlation with soil type and salinity. Furthermore, unfrozen water content below 0°C is also estimated and uncertainty of estimation is analyzed.
Endoplasmic reticulum stress drives macrophages to produce IL-33 to favor Th2 polarization in the airways
Authors: Xiao Yuan , Zhang Huangping , Liu Yu , Mo Lihua , Liao Yun , Huang Qinmiao , Yang Liteng , Zhou Caijie , Liu Jiangqi , Sun Xizhuo , Yu Haiqiong , Yang Pingchang 
Published: March 2024

Abstract
Interleukin (IL)-33 is a key driver of T helper 2 (Th2) cell polarization. Endoplasmic reticulum (ER) stress plays a role in the skewed T cell activation. The objective of this project is to elucidate the role of IL-33 derived from macrophages in inducing Th2 polarization in the airways. In this study, bronchoalveolar lavage fluids (BALF) were collected from patients with asthma and healthy control subjects. Macrophages were isolated from the BALF by flow cytometry cell sorting. An asthmatic mouse model was established using the ovalbumin/alum protocol. The results showed that increased IL33 gene activity and ER stress–related molecules in BALF-derived M2a macrophages was observed in asthmatic patients. Levels of IL33 gene activity in M2a cells were positively correlated with levels of asthma response in asthma patients. Sensitization exacerbated the ER stress in the airway macrophages, which increased the expression of IL-33 in macrophages of airway in sensitized mice. Conditional ablation of Il33 or Perk or Atf4 genes in macrophages prevented induction of airway allergy in mice. In conclusion, asthma airway macrophages express high levels of IL-33 and at high ER stress status. Inhibition of IL-33 or ER stress in macrophages can effectively alleviate experimental asthma.
Understanding health behaviors that modify the risk for obesity in ADHD
Authors: Roberts Delanie K , Sarver Dustin E , Cash Annah R , Walker Benjamin H , Lim Crystal S 
Published: March 2024

Abstract

Objective
Research provides support for the associated risk of inadequate sleep duration, limited physical activity, and excessive media use in attention-deficit/hyperactivity disorder (ADHD) and obesity. The present study aims to (1) examine the association between ADHD and overweight or obese status (OW/OB); (2) comprehensively examine sleep duration, physical activity, and media use as potential moderators of OW/OB; and (3) examine the moderating effects of these health behaviors cross-sectionally by comparing medicated youth with ADHD, unmedicated youth with ADHD, and youth without ADHD.

Methods
Data were acquired from the 2018 and 2019 National Survey of Children’s Health, a nationally representative survey of caregivers conducted across the United States. The current study used data for youth 11–17 years old with a final sample size of 26,644. Hours of sleep, physical activity, and media use per day were dichotomized based on national recommendation guidelines for each health behavior (i.e., either meeting or not meeting guidelines).

Results
The OW/OB prevalence rate was 7% greater among unmedicated youth with ADHD than among medicated youth with ADHD. Medicated youth with ADHD and peers without ADHD had similar OW/OB rates. Among medicated youth with ADHD, physical activity, sleep duration, and media use did not contribute to OW/OB risk after controlling for family poverty level. However, among unmedicated youth with ADHD, meeting sleep duration guidelines was linked to a lower OW/OB risk.

Conclusion
Overall, findings suggest that clinical providers and parents may wish to prioritize improved sleep duration in the management of OW/OB risk in youth with ADHD.