bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantitative Superresolution Imaging of F-Actin in the Cell Body and Cytoskeletal Protrusions Using Phalloidin-Based Single-Molecule Labeling and Localization Microscopy
Authors: Gunasekara Hirushi , Perera Thilini , Chao Chih-Jia , Bruno Joshua , Saed Badeia , Anderson Jesse , Zhao Zongmin , Hu Ying S. 
Published: March 2024

ABSTRACTWe present single-molecule labeling and localization microscopy (SMLLM) using dye-conjugated phalloidin to achieve enhanced superresolution imaging of filamentous actin (F-actin). We demonstrate that the intrinsic phalloidin dissociation enables SMLLM in an imaging buffer containing low concentrations of dye-conjugated phalloidin. We further show enhanced single-molecule labeling by chemically promoting phalloidin dissociation. Two benefits of phalloidin-based SMLLM are better preservation of cellular structures sensitive to mechanical and shear forces during standard sample preparation and more consistent F-actin quantification at the nanoscale. In a proof-of-concept study, we employed SMLLM to super-resolve F-actin structures in U2OS and dendritic cells (DCs) and demonstrate more consistent F-actin quantification in the cell body and structurally delicate cytoskeletal proportions, which we termed membrane fibers, of DCs compared to direct stochastic optical reconstruction microscopy (dSTORM). Using DC2.4 mouse dendritic cells as the model system, we show F-actin redistribution from podosomes to actin filaments and altered prevalence of F-actin-associated membrane fibers on the culture glass surface after lipopolysaccharide exposure. While our work demonstrates SMLLM for F-actin, the concept opens new possibilities for protein-specific single-molecule labeling and localization in the same step using commercially available reagents.
Reconciling the importance of minerals for propagation of antibiotic resistance genes in the environment
Authors: Hendiani Saghar , Moral Carlota Carbajo , Hansen Mads Frederik , Adebayo Agbaje Oluwatoosin Bunmi , Arellano Caicedo Pablo Nicolas , Verma Taru , Mulec Ines Mandić , Burmølle Mette , Sand Karina Krarup 
Published: March 2024

ABSTRACTThe role of mineral surfaces in environmental processes, particularly their influence on DNA preservation, biofilm formation, and genetic transfer, has garnered attention due to its implications for the spread of antibiotic resistance genes (ARg). Despite the recognized significance of mineral-mediated DNA transfer, this mechanism remains poorly understood. Here we investigate the intricate interplay between soil minerals, bacteria, and DNA, to better understand the mechanisms driving ARg propagation in natural environments. We here study the uptake of mineral adsorbed DNA into the natural competent bacteriab. subtilisand further explore the influence of minerals on the viability and subsequent biofilm formation of bothb. subtilisandA. baylyi.We further adsorbed DNA to mineral surfaces and allowed biofilm formation while monitoring the propagation of the ARg through out the biofilms. All the results are set in context of mineral surface properties such as surface charge, charge densities and surface area.Our results showed that the surface properties of the mineral surfaces are highly influencing the transformation efficiencies, viability and biofilm formation where in particular a high number of positive charged surface sites enhance biofilm formation and viability and inhibit transformation. The influence of the mineral surfaces diminishes as the biofilm develops and propagation of mineral adsorbed ARg are seen widely across the mineral surfaces. Our results have implication for mitigations strategies and reconcile mineral surfaces as hot spots for the propagation of antibiotic resistance-which indeed can be driven by transformation in the absence of bacteria carrying the traits. In principle all it takes is one successful transfer event from a mineral adsorbed ARg.
Mental illness and COVID-19 vaccination: a multinational investigation of observational & register-based data
Authors: Barker Mary M , Kõiv Kadri , Magnúsdóttir Ingibjörg , Milbourn Hannah , Wang Bin , Du Xinkai , Murphy Gillian , Herweijer Eva , Gísladóttir Elísabet U , Li Huiqi , Lovik Anikó , Kähler Anna K. , Campbell Archie , Feychting Maria , Hauksdóttir Arna , Joyce Emily E , Thordardottir Edda Bjork , Frans Emma M. , Hoffart Asle , Mägi Reedik , Tómasson Gunnar , Ásbjörnsdóttir Kristjana , Jakobsdóttir Jóhanna , Andreassen Ole A. , Sullivan Patrick F. , Johnson Sverre Urnes , Aspelund Thor , Brandlistuen Ragnhild Eek , Ask Helga , McCartney Daniel L , Ebrahimi Omid V , Lehto Kelli , Valdimarsdóttir Unnur A , Nyberg Fredrik , Fang Fang 
Published: March 2024

ABSTRACTBackgroundIndividuals with mental illness are at higher risk of severe COVID-19 outcomes. However, previous studies on the uptake of COVID-19 vaccination in this population have reported conflicting results. Therefore, we aimed to investigate the association between mental illness and COVID-19 vaccination uptake, using data from five countries.MethodsData from seven cohort studies (N=325,298), and the Swedish registers (8,080,234), were used to identify mental illness and COVID-19 vaccination uptake. Multivariable modified Poisson regression models were conducted to calculate the prevalence ratio (PR) and 95% CIs of vaccination uptake among individuals with v.s. without mental illness. Results from the cohort studies were pooled using random effects meta-analyses.FindingsMost of the meta-analyses performed using the COVIDMENT study population showed no significant association between mental illness and vaccination uptake. In the Swedish register study population, we observed a very small reduction in the uptake of both the first (prevalence ratio [PR]: 0.98, 95% CI: 0.98-0.99, p<0.001) and second dose among individuals with mental illness; the reduction was however greater among those not using pyschiatric medication (PR: 0.91, 95% CI: 0.91-0.91, p<0.001).ConclusionsThe high uptake of COVID-19 vaccination observed among individuals with most types of mental illness highlights the comprehensiveness of the vaccination campaign, however lower levels of vaccination uptake among subgroups of individuals with unmedicated mental illness warrants attention in future vaccination campaigns.
First report of astroviruses in Tanzanian bats
Authors: Joffrin Léa , Iliopoulou Evangelia , Falzon Marta , Sabuni Christopher , Kirkpatrick Lucinda , De Bruyn Luc 
Published: March 2024

AbstractEmerging and re-emerging infectious diseases have posed significant global health threats, with many attributed to zoonotic RNA viruses. These pathogens can, under some conditions, cross species barriers, facilitating transmission from animal hosts to humans. Bats, characterised by unique physiological and ecological features, and remarkable species diversity, are recognized to host numerous viruses with cross-species transmission potential. This study aimed to investigate the presence of RNA viruses from a broad diversity of Tanzanian bats while valorising archived biological samples. RNA was extracted from 125 samples (28 faeces and 97 oral swabs) of 17 bat species, followed by PCR amplification targeting five distinct viral genera (Filovirus, Coronavirus, Hantavirus, Paramyxovirus and Astrovirus). Overall, 1.6 % (3/125) of the samples from two bat species (Scotophilus dinganiiandMiniopterus fraterculus) tested positive for astrovirus, with the coinfection of one bat with two AstV strains. No samples tested positive for Filovirus, Coronavirus, Hantavirus and Paramyxovirus. Phylogenetic analysis based on RNA-dependent RNA polymerase sequences revealed these sequences are respectively clustering with astroviruses detected in other bat species from the genus Scotophilus from East Asia and with astroviruses detected inMiniopterusbats from Africa and Asia. Altogether, these results are the first report of astroviruses in Tanzanian bats.
Non-viral Intron Knockins Enable Simplified and Flexible Targeting of Endogenous Genes
Authors: Roth Theodore L. , Lu Johnathan , McClellan Alison , Takacsi-Nagy Oliver , Satpathy Ansuman T. 
Published: March 2024

ABSTRACTTargeting new genetic material to endogenous genes has opened diverse therapeutic and research applications, but current exon-based targeting methods have limited integration sites and are compatible only with complex or harsh selection methods. We present non-viral intron targeting, integrating large synthetic exons into endogenous introns to increase targeting flexibility and simplify selection of successfully edited cells. Engineered control of large synthetic exon’s splicing behavior further generalizes cell and gene therapy applications of non-viral intron knockins.
Prior exposure to pathogens augments host heterogeneity in susceptibility and has key epidemiological consequences
Authors: Hawley Dana M. , Pérez-Umphrey Anna A. , Adelman James S. , Fleming-Davies Arietta E. , Garrett-Larsen Jesse , Geary Steven J. , Childs Lauren M. , Langwig Kate E. 
Published: March 2024

AbstractPathogen epidemics are key threats to human and wildlife health. Across systems, host protection from pathogens following initial exposure is often incomplete, resulting in recurrent epidemics through partially-immune hosts. Variation in population-level protection has important consequences for epidemic dynamics, but how acquired protection influences inter­individual heterogeneity in susceptibility and its epidemiological consequences remains understudied. We experimentally investigated whether prior exposure (none, low-dose, or high­dose) to a bacterial pathogen alters host heterogeneity in susceptibility among songbirds. Hosts with no prior pathogen exposure had little variation in protection, but heterogeneity in susceptibility was significantly augmented by prior pathogen exposure, with the highest variability detected in hosts given high-dose prior exposure. An epidemiological model parameterized with experimental data found that heterogeneity in susceptibility from prior exposure more than halved epidemic sizes compared with a homogeneous population with identical mean protection. However, because infection-induced mortality was also greatly reduced in hosts with prior pathogen exposure, reductions in epidemic size were smaller than expected in hosts with prior exposure. These results highlight the importance of variable protection from prior exposure and/or vaccination in driving population-level heterogeneity and epidemiological dynamics.
TALEN-mediated intron editing of HSPCs enables transgene expression restricted to the myeloid lineage
Authors: Seclen Eduardo , Jang Jessica C. , Lawal Aminah O. , Pulicani Sylvain , Boyne Alex , Tkach Diane , Juillerat Alexandre , Duchateau Philippe , Valton Julien 
Published: March 2024

AbstractGene therapy in hematopoietic stem and progenitor cells (HSPCs) shows great potential for the treatment of inborn metabolic diseases. Typical HSPC gene therapy approaches rely on constitutive promoters to express a therapeutic transgene, which is associated with multiple disadvantages. Here, we propose a novel promoter-less intronic gene editing approach that triggers transgene expression only after cellular differentiation into the myeloid lineage. We integrated a splicing-competent eGFP cassette into the first intron ofCD11band observed expression of eGFP in the myeloid lineage but minimal to no expression in HSPCs or differentiated non-myeloid lineages.In vivo, edited HSPCs successfully engrafted in immunodeficient mice and displayed transgene expression in the myeloid compartment of multiple tissues. Using the same approach, we expressed alpha-L-iduronidase (IDUA), the defective enzyme in Mucopolysaccharidosis type I, and observed a 10-fold supraendogenous IDUA expression exclusively after myeloid differentiation. Edited cells efficiently populated bone marrow, blood, and spleen of immunodeficient mice, and retained the capacity to secrete IDUAex vivo. Importantly, cells edited with the eGFP and IDUA transgenes were also found in the brain. This approach may unlock new therapeutic strategies for inborn metabolic and neurological diseases that require the delivery of therapeutics in brain.
Combining topic modeling and bibliometric analysis to understand the evolution of technological innovation adoption in the healthcare industry
Authors: Cobelli Nicola , Blasi Silvia 
Published: March 2024

PurposeThis paper explores the Adoption of Technological Innovation (ATI) in the healthcare industry. It investigates how the literature has evolved, and what are the emerging innovation dimensions in the healthcare industry adoption studies.Design/methodology/approachWe followed a mixed-method approach combining bibliometric methods and topic modeling, with 57 papers being deeply analyzed.FindingsOur results identify three latent topics. The first one is related to the digitalization in healthcare with a specific focus on the COVID-19 pandemic. The second one groups up the word combinations dealing with the research models and their constructs. The third one refers to the healthcare systems/professionals and their resistance to ATI.Research limitations/implicationsThe study’s sample selection focused on scientific journals included in the Academic Journal Guide and in the FT Research Rank. However, the paper identifies trends that offer managerial insights for stakeholders in the healthcare industry.Practical implicationsATI has the potential to revolutionize the health service delivery system and to decentralize services traditionally provided in hospitals or medical centers. All this would contribute to a reduction in waiting lists and the provision of proximity services.Originality/valueThe originality of the paper lies in the combination of two methods: bibliometric analysis and topic modeling. This approach allowed us to understand the ATI evolutions in the healthcare industry.
Spatial structure and influencing factors of an emerging wine tourism network: a case study of the Ningxia wine region
Authors: Gu Qiushi , Ye Ben Haobin , Huang Songshan (Sam) , Wong Man Sing , Wang Lei 
Published: March 2024

Purpose
Networks linking tourist attractions or organizations are a major focus of tourism research. Despite extensive research on tourism networks, academic research on the spatial structure and formation of wine tourism networks is limited. This study aims to investigate the spatial structure and factors influencing the development of a network among Ningxia wineries, an emerging wine tourism destination in China.

Design/methodology/approach
This study uses social network analysis to uncover “what” the spatial structure of wine tourism networks looks like. Sixteen in-depth interviews were conducted among key stakeholders to explain the “why” of such structural characteristics.

Findings
The results show that in an emerging wine tourism destination, popular tourist attractions enjoy high centrality and hold key positions in the wine tourism network. Small wineries exhibit high closeness centrality, and only one winery serves as a network broker. According to the stakeholders, the importance of network actors will increase as their economic and political importance increase, while small wineries that lack differentiation in the network may perish.

Practical implications
Local governments can implement the suggested measures for improving network connections, and wineries are advised to find suitable positions to improve the experiences of tourists.

Originality/value
This study pioneers the identification of the distinct structure and factors influencing the network of an emerging wine tourism destination, thus enriching the understanding of the interplay and roles of different actors.
Institutional pluralism and the implementation of women’s enterprise policy
Authors: Mallett Oliver , Wapshott Robert , Wilson Nazila 
Published: March 2024

PurposeThis research paper generates new insights into the challenges of implementation in women’s enterprise policy. It argues that organisations involved in policy implementation need to be understood as operating in a context of institutional pluralism and answers: How do organisations involved in the implementation of women’s enterprise policy manage the challenges of institutional pluralism?Design/methodology/approachAddressing the need for women’s enterprise policy to learn from the past, the research adopts a historical approach to the study of policy implementation through examination of the UK’s Phoenix Development Fund (1999–2008). It analyses a wide range of secondary sources to examine 34 projects funded and supported by the Phoenix Development Fund that targeted women entrepreneurs.FindingsPotentially conflicting institutional logics associated with central government, mainstream business support and local communities were managed through four key processes: dominance; integration; constellation and bridging. The management of institutional pluralism was effective in delivering support to communities but not in providing an effective platform for learning in government or establishing sustainable, long-term mechanisms.Originality/valueThe paper develops an empirical contribution to practice through identification of processes to manage the challenges of institutional pluralism and lessons for community-engaged policy implementation. A theoretical contribution to academic debates is provided by the conceptualisation of these challenges in terms of institutional pluralism and the novel concept of institutional bridging. The study also demonstrates the value of historical methods for women’s enterprise policy to learn the lessons of the past.
“I didn’t want to leave my country” – mental health services as experienced by Bosnian refugees in Australia
Authors: Karakas Gabriella , du Plooy Daniel R. 
Published: March 2024

Purpose
Despite the substantial body of international research exploring the effects of forced migration on mental health and the obstacles faced by refugee cohorts in accessing mental health services, there is a lack of in-depth studies specifically focusing on Bosnian refugees in Australia – one of the largest ethnic groups of displaced peoples. This qualitative investigation seeks to convey the experiences of mental health services by five Bosnian refugees in Melbourne, highlighting perceived pathways and barriers to service utilisation. This study aims to address two primary research questions: firstly, what characterises the experiences of Bosnian refugees in Australia when accessing mental health services? Secondly, what are the main barriers encountered by this population when seeking mental health services?

Design/methodology/approach
This study used qualitative inquiry and in-depth interviews to investigate the experiences of Bosnian refugees with mental health services in Australia. Data was collected through face-to-face interviews with five Melbourne-based Bosnian refugees who had previously accessed mental health services. Participants were recruited from community groups or associations using purposive sampling. Thematic analysis was used.

Findings
Key themes were revealed, such as the crucial role of social and community services, preferences for individual vs group therapy, potential re-traumatisation from therapeutic engagement, distrust of government-run mental health services and concerns regarding psychopharmaceutical prescription practices. Addressing barriers to mental health service access necessitates a multifaceted approach, including flexible social and community service support, an increased number of co-ethnic professionals and a recognition of cultural variations for effective service provision. Implementing these strategies can enhance help-seeking behaviours, provide culturally appropriate mental health services and improve the experiences of Bosnian refugees in Australia.

Originality/value
To the best of the authors’ knowledge, this study is the first to qualitatively explore how Bosnian refugees in Melbourne perceived the adequacy and availability of mental health services upon arrival to Australia. They are a large and potentially vulnerable community, due to experiences of war trauma and dislocation from country of origin. A lack of understanding regarding how this cohort engage with mental health services can lead to persistent inequities and ineffective service provision. This study identifies unique experiences and perspectives of Bosnian refugee participants, including distrust of government-run mental health services, and concerns regarding psychopharmaceutical prescription practices. This research is expected to contribute positively to the discourse on mental health service provision for Bosnian refugees and wider refugee communities in Australia.
Education for the circular economy in higher education: an overview of the current state
Authors: Renfors Sanna-Mari 
Published: March 2024

Purpose
Higher education institutions and their lecturers are strategic agents and main drivers that contribute to circular economy transition. This requires them to understand the key circular economy competencies and how to integrate circular economy holistically into their curricula with the suitable teaching and learning approaches. This study aims to support them by providing an overview on the characteristics of education for the circular economy (ECE) and suggestions to lecturers to further develop their curricula.

Design/methodology/approach
The data consisted of scientific articles (n = 22) describing circular economy courses in higher education. Qualitative content analysis with quantitative features was performed on the selected articles to answer the research question.

Findings
The findings confirm that the system’s focus is the key issue in ECE. However, to integrate circular economy holistically into the curricula, ECE should be implemented more widely in the context of different industries and market contexts to find innovative teaching and learning approaches. The demand side needs to be incorporated in the courses, as systemic transformation is also about transforming consumption. All levels of implementation and circular economy objectives should be included in courses to promote systems thinking. In addition, innovative forms of real workplace interaction should be increased.

Originality/value
As ECE has started to emerge as a new field of study, this article provides the first integrated overview of the topic.
GraphQL response data volume prediction based on Code2Vec and AutoML
Authors: Zhang Feng , Wei Youliang , Feng Tao 
Published: March 2024

Purpose
GraphQL is a new Open API specification that allows clients to send queries and obtain data flexibly according to their needs. However, a high-complexity GraphQL query may lead to an excessive data volume of the query result, which causes problems such as resource overload of the API server. Therefore, this paper aims to address this issue by predicting the response data volume of a GraphQL query statement.

Design/methodology/approach
This paper proposes a GraphQL response data volume prediction approach based on Code2Vec and AutoML. First, a GraphQL query statement is transformed into a path collection of an abstract syntax tree based on the idea of Code2Vec, and then the query is aggregated into a vector with the fixed length. Finally, the response result data volume is predicted by a fully connected neural network. To further improve the prediction accuracy, the prediction results of embedded features are combined with the field features and summary features of the query statement to predict the final response data volume by the AutoML model.

Findings
Experiments on two public GraphQL API data sets, GitHub and Yelp, show that the accuracy of the proposed approach is 15.85% and 50.31% higher than existing GraphQL response volume prediction approaches based on machine learning techniques, respectively.

Originality/value
This paper proposes an approach that combines Code2Vec and AutoML for GraphQL query response data volume prediction with higher accuracy.