bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cerebrospinal fluid viral escape in HIV patients on antiretroviral therapy: A systematic review of reported cases
Authors: Nyondo Goodluck G. , Njiro Belinda J. , Bwire George M. 
Published: April 2024

AbstractCerebrospinal fluid (CSF) viral escape rarely occurs when HIV is detected in the CSF, while it is undetectable in the blood plasma or detectable in CSF at levels that exceed those in the blood plasma. We conducted this review to comprehensively synthesise its clinical presentation, diagnosis, management strategies and treatment outcomes. A review registered with PROSPERO (CRD42023475311) searched evidence across PubMed/MEDLINE, Embase, Web of Science, Scopus, and Google Scholar to gather articles (case reports/series) that report on CSF viral escape in people living with HIV (PLHIV) on antiretroviral therapy (ART). The quality of studies was assessed based on the domains of selection, ascertainment, causality, and reporting. A systematic search identified 493 articles and 27 studies that include 21 case reports, and six case series were involved in the review. The studies reported 62 cases of CSF viral escape in PLHIV. The majority were men (66.67%), with a median age of 43 (range: 28–73) years. Approximately, 31 distinct symptoms were documented, mostly being cognitive dysfunction, gait abnormalities, and tremors (12.51%). Diagnosis involved blood and CSF analysis, magnetic resonance imaging, and neuropsychological assessments. Over 36 ART regimens were employed, with a focus on ART intensification; almost one‐third of the regimens contained Raltegravir (integrase strand transfer inhibitor). The outcomes showed 64.49% full recovery, 30.16% partial recovery, and 4.76% died. When neuropsychological symptoms manifest in PLHIV, monitoring for CSF viral escape is essential, regardless of plasma viral suppression. Personalised treatment strategies, particularly ART intensification, are strongly advised for optimising treatment outcomes in PLHIV diagnosed with CSF HIV escape.
Broadscale and fine‐scale variables predict the occurrence of a stream‐breading bufonid: Habitat use by the Arizona toad (Anaxyrus microscaphus)
Authors: Montgomery Brett J. , Bateman Heather L. , Albuquerque Fábio S. D. 
Published: April 2024

AbstractArizona toad (Anaxyrus microscaphus) is a stream‐breeding bufonid of conservation concern in Arizona, New Mexico, Utah, and Nevada. We determined the occupancy and habitat use of the Arizona toad throughout its range. We surveyed 500‐m reaches along perennial and intermittent streams during the summers of 2021 and 2022 (n = 232) in Arizona. We recorded the presence of all toad life stages, focusing on larval stages. We related toad occupancy to broadscale environmental variables, including measures of bioclimate, habitat heterogeneity, solar radiation, and topography. We collected fine‐scale variables to summarize vegetation cover and substrate within plots (n = 53). We applied multiple occupancy models. Single‐species model results found low toad occupancy with high detection. Two principal component analyses (PCA) were run on broadscale and fine‐scale variables to reduce the number of variables included in the models. Toad occupancy was best predicted by top models with bioclimatic components; occupancy decreases with extremely hot temperatures and less precipitation. A logistic regression related toad presence to fine‐scale components with top models describing riparian complexity and algae. Arizona toads were selected for areas with foliar canopy cover, shallow water, algae cover, and pebble substrates. Arizona toad is an uncommon species and maintaining riparian forests of complex habitats with shallow and side‐channel flow will be important for toad conservation. Implications of less water cause riparian habitat to dry, which ultimately harms aquatic life.
The Extrema of Japanese Literacy: Beyond the Bounds of Average Reading
Authors: Hemelstrand Shawn , Inoue Tomohiro 
Published: April 2024

AbstractThe unique orthographic complexities of Japanese, which utilizes multiple types of scripts (morphographic kanji and syllabic hiragana and katakana) for the same spoken language, place unique demands on early learners. Much research has centered on the average ability of Japanese readers, but given the varying challenges of these scripts, attention may need to be oriented towards the outer bounds of ability, specifically those who both deeply struggle (including dyslexia) and excel at reading. In this article, we provide a review of past research on Japanese literacy along with a pre‐registered analysis using quantile generalized additive models that tested two theories in cross‐language literacy research, the orthographic depth and breadth hypotheses. Our results confirmed that the relationships between cognitive skills (phonological awareness and morphological awareness) and reading outcomes differed by script (hiragana vs. kanji). However, their relationships varied in curvilinearity and associations seemed to change based on reading ability. These findings provide supporting evidence for the general scope of the orthographic depth and breadth hypotheses, but may ultimately question the uniformity of these theories.
Estimating the eco‐efficiency of urban waste services towards sustainable waste management
Authors: Molinos‐Senante María , Maziotis Alexandros , Sala‐Garrido Ramón 
Published: April 2024

AbstractAssessing the eco‐efficiency of municipalities regarding the provision of solid waste services is a useful tool for improving its sustainability. However, robust, and reliable methods are needed to benchmark the performance of municipalities. Data envelopment analysis (DEA) methods typically used to assess the eco‐efficiency of municipal solid waste (MSW) service providers. However, the variables used in the eco‐efficiency assessment bear distinct weights for each individual unit, limiting thus interpretation. Therefore, benchmarking the performance of MSW service providers becomes challenging because different conditions are applied. Thus, this study uses a common set weights DEA (DEA‐CSW) model assess the eco‐efficiency of different municipalities in providing MSW services. Eco‐efficiency scores were estimated by integrating total costs, recycled waste and unsorted waste as input, desirable outputs and undesirable outputs, respectively. The empirical application of the model demonstrated that when using DEA‐CSW, only one municipality (out of 36) was eco‐efficient in managing MSW. The weightings for inputs and outputs were allocated based on the degree of satisfaction estimated for each municipality. Satisfaction ranged from 0.829 to 1.000 indicating that none of the municipalities were heavily penalized based on the eco‐efficiency scores estimation when allocating common weights. In conclusion, this study demonstrates that compared to traditional DEA models, the DEA‐CSW approach is more adequate at benchmarking the performance of municipalities regarding the provision of MSW services.
Sulphonic Acid Functionalized Ionic Liquids as an Efficient Catalyst for the Synthesis of Digeranyl Ethers and Geranyl Phenols
Authors: Bhumkar Vikram G. , Nahar Vrushabh S. , Sakate Sachin S. 
Published: April 2024

AbstractAn alternate synthetic approach was developed for the synthesis of the digeranyl ether and naturally occurring biologically active geranyl phenols using −SO3H functionalized ionic liquids within 0.5–1.5 h with better yields. This approach was further explored for different naturally occurring activated phenols with geraniol yielding geranyl phenols via alkylation at ambient temperature using ionic liquids as catalyst. The synthesized ionic liquids were characterized using Py‐IR studies to establish the Brønsted acidity and the Hammett acidity characteristics of all the four ionic liquids. Amongst these ionic liquids IL‐1 exhibited beneficial catalytic activity towards the synthesis of digeranyl ether and geranyl phenols with excellent yields. Reusability studies indicated that IL‐1 was recycled for seven times without losing the catalytic activity which was further corroborated with pre‐ and post‐characterization of IL‐1. This work also highlights the alternate route for the synthesis of the cannabinoid compounds and their analogues.
Oxalohydrazide‐Based Chemosensor for Detection of Cu2+/Fe3+ Ions and Its Application in Live Cell Imaging
Authors: Murugaperumal Parvathavarthini , Rajendran Praveena , Nallathambi Sengottuvelan , Ayyanar Siva , Perdih Franc , Chellappan Selvaraju 
Published: April 2024

AbstractThe N1,N2‐diphenyloxalohydrazide OX(HA)2 chemosensor was synthesized and used as a colorimetric and fluorescent receptor to detect Cu2+ and Fe3+ ions in the presence of other metal ions. A range of physicochemical approaches were utilized to characterize the symmetrical oxalohydrazide‐based receptor, including FT‐IR, 1H and 13C NMR, TGA, and mass spectrometry. According to single crystal X‐ray diffraction investigation, OX(HA)2 crystallizes in monoclinic with space group C2/c. UV‐Vis and fluorescence spectroscopy were used to thoroughly explore OX(HA)2’s sensing capabilities towards diverse cations. Using measurements of fluorescence intensity, the binding constant for OX(HA)2 with Cu2+/Fe3+ ions was calculated to be 2.78×103 M−1 and 1.12×103 M−1 with the lower detection limit of 0.43 μM and 0.2 μM respectively. With a 1 : 1 binding mechanism validated by Job's plot, mass spectral analysis, FT‐IR spectra, and density functional theory, the dual‐sensing receptor exhibits remarkable selectivity and sensitivity towards Cu2+ and Fe3+ ions. The average fluorescence lifetimes of OX(HA)2, OX(HA)2+Cu2+, and OX(HA)2+Fe3+ were calculated to be 2.19 ns, 1.73 ns, and 1.26 ns, respectively. This fluorescence lifetime measurement results strongly support the complexation of Cu2+ and Fe3+. When Cu2+ and Fe3+ ions bind with OX(HA)2, they induce the ‘Turn‐Off’ signal. Then, fluorescence imaging of the OX(HA)2 receptor in MDA‐MB‐231 demonstrated that the receptor holds a lot of promise for usage in bioimaging and their IC50 value for cytotoxicity is 96.96 μg. Furthermore, fluorescence microscopy experiments demonstrate that OX(HA)2 can be utilized as a fluorescent probe for detecting Cu2+ and Fe3+ ions in living cells, making it potentially helpful as an anticancer medication.
Facile Synthesis of Copper Telluride Nanoparticles as a Robust Nanoenzymatic Catalyst for Intrinsic Peroxidase‐Like Activity
Authors: Chauhan Nandita , Sharma Nikhil , Buhari Salahuddeen , Das Prabhu Prasad , Kumar Sanjay , Chalana Ashish 
Published: April 2024

AbstractCopper telluride nanoparticles are classified as the family of metal chalcogenides and were synthesized using a simple solvothermal method using a new source of telluride, ditelluride diphenyl. The characterized techniques revealed that the atomic composition of copper tellurides is Cu2Te and particles are spherical. This study is greatly examined by different designed experiments for the proof of the intrinsic peroxidase‐like activity of copper telluride nanoparticles and its effectiveness in a variety of similar structures. Cu2Te nanoparticles can convert peroxidase substrates (TMB and OPD) to colored products in the presence of H2O2. In addition, the as‐prepared nanoparticles have the potential to oxidize DPPH and ABTS substrates. Michaelis–Menten constant KM value for TMB and H2O2 was found to be 12 mM and 0.60 mM for H2O2 and the Vmax value of the Cu2Te was found to be TMB and H2O2 was 2.50×10−7 and 0.82×10−8 MS−1. These data are found to be almost comparable with natural peroxidase HRP and other artificial enzymes reported in the literature. The natural peroxidase‐like activities of Cu2Te nanoparticles originated from the catalytic decomposition of H2O2 into ⋅OH radical, which was confirmed from the ESR spin‐trapping technique and using a fluorescence probe, terephthalic acid.
A Sustainable Combustion Process for Green Synthesis of TiO2 Nanoparticles: Applications in Photocatalytic Degradation and Electrochemical Sensing for Environmental Remediation
Authors: Basavaraja Avinash , Ravikumar Chunchanakuppe R. , Khan Afaq A. , Khasim Syed , Vasudeva Bhoomika , Sharma Sadanand , Santosh Mysore S. 
Published: April 2024

AbstractHerein, a sustainable approach to combustion has been presented, utilizing Aloe Vera latex as a reducing agent in synthesizing titanium dioxide (TiO2) nanoparticles. The phase and composition of TiO2 have been confirmed using X‐ray diffraction, while the band gap has been determined as 3.17 eV using the Kubelka‐Monk plot. The photocatalytic potential of the TiO2 has been explored by assessing their effectiveness in degrading AR‐88 dye, demonstrating significant activity at 500 nm. Remarkably, in a span of 120 min of UV radiation, AR‐88 dye could reach an impressive photodegradation rate of 98.6 %, rendering the dye nearly colorless. TiO2 NPs have also been investigated as an electrode material in sensing paracetamol and D‐glucose by coating them onto a carbon paste electrode and immersing them in a 1 M KOH solution. The cyclic voltammetry experiments reveal that the TiO2 NPs possess excellent electrochemical characteristics, with a calculated proton diffusion coefficient of 5.651×10−4 cm2 s−1. These findings underscore the potential of TiO2 as a highly desirable electrode material with remarkable electrochemical properties. This research opens new avenues for utilizing TiO2 NPs in various electrochemical applications, particularly in the detection of pharmaceutically important compounds, highlighting its versatility.
First Sublimation Thermodynamics and First Gas‐Phase Experimental Molecular Structure of Heteroporphyrins: 5,10,15,20‐Tetraphenyl‐21‐X, 23‐Y‐Porphyrins (X=O or S; Y=N or S)
Authors: Kuzmin Ilya A. , Shlykov Sergey A. , Krasnov Alexander V. , Pukhovskaya Svetlana G. , Syrbu Sergey A. , Ivanova Yulia B. 
Published: April 2024

AbstractFirst sublimation enthalpies of heteroporphyrins: 10,15,20‐tetraphenyl‐21‐oxa‐ (HOPP), 5,10,15,20‐tetraphenyl‐21‐thia‐ (HSPP) and 5,10,15,20‐tetraphenyl‐21,23‐dithia‐ (S2PP) were measured by Knudsen effusion mass spectrometry: ΔsublH (T)=209±2 (561 K), 224±2 (552 K) and 219±2 (577 K) kJ/mol, respectively. Geometry and force field of a free molecule of HSPP was studied by quantum chemistry, and first gas‐phase experimental molecular structure of heteroporphyrins was derived by gas‐phase electron diffraction. Theoretically observed changes of the coordination cavity geometries due to heteroatomic intracavity substitution were experimentally confirmed with good agreement. Molecular structures of the dimeric associates of the heteroporphyrins were tested simulated to reveal tendencies in sublimation thermodynamics.
Beyond Acetylene: Exploring Solvent Stabilization of Calcium Carbide‐derived Acetylide Intermediates and Their Significance in Small Organic Molecule Synthesis
Authors: Hainer Andrew S. , Lanterna Anabel E. , Scaiano Juan C. 
Published: April 2024

AbstractThe reaction of calcium carbide with water has been widely used as a source of acetylene for the synthesis of organic molecules. In contrast with this traditional approach, we use the CaC2 reaction under water‐starved conditions and show reactions that involve a calcium acetylide intermediate, reminiscent of Grignard reaction intermediates, which are stabilized by the solvent. Using this reaction pathway may allow for new chemistry, finer reaction control, and safety by limiting the formation of acetylene gas during the reaction. For example, reaction with bromoarenes, readily yields the corresponding diaryl acetylene.
High Singlet Oxygen Generating Benzothienyl‐[b]‐fused BODIPY Nanoaggregates and Photoinduced Apoptosis in Human Carcinoma
Authors: Mishra Sneha , Shelar Sandeep B. , Barick K. C. , Hassan P. A. , Agarwal Neeraj 
Published: April 2024

AbstractHeavy atom free organic materials absorbing in red to near infrared (NIR) region are potential candidates as photosensitizers (PS) in photodynamic therapy (PDT). PDT relies on many factors and one of them is singlet oxygen generation ability of PS in aqueous medium. In this contribution, we report the synthesis of a new PS i. e., 8‐thienyl bisbenzothieno‐[b]‐fused‐boron‐dipyrromethene (BT‐[b]‐fused BODIPY). This PS shows absorption (λabs) at ~650 nm in toluene while in its water‐dispersible nanoaggregates it is found at ~764 nm. Nanoaggregates of BT‐[b]‐fused BODIPY demonstrated dual advantages, (i) facilitating PS release in the cellular system and (ii) sharply enhanced singlet oxygen efficiency (
~48 %) in aqueous medium. Intracellular reactive oxygen species (ROS) generation was confirmed by 2′,7′‐dichlorodihydrofluorescein diacetate (DCFDA) assay in A549 cells. In vitro photocytotoxic studies of BT‐[b]‐fused BODIPY are carried out in different carcinomas i. e., MCF‐7 and A549 cells. It has zero dark cell toxicity while half maximal inhibitory concentration (IC50) in MCF‐7 is found to be low (6.08±1.1) μM and 7.41±1.9 μM in A549 on 15 min light exposure. These nanoaggregates induce apoptosis in cancer cells on light irradiation thereby proving it to be a potential PS for PDT.
Characterization and Drug Release Properties of Curcumin Loaded Native and Nano‐Sized Starches of Pigeon Pea (Cajanus cajan) and Bambara Nut (Vigna subterranean)
Authors: Balogun‐Agbaje Olalekan Adeyinka , Odeniyi Michael Ayodele 
Published: April 2024

AbstractStarches from underutilized sources such as Bambara nut (BAM) and pigeon pea (PP) can serve as new sources of starch. Starch nanoparticles (SNP) of BAM (BAMnp) and PP (PPnp) are prepared by nanoprecipitation. The physicochemical and pasting properties of the native and SNP are evaluated. The scanning electron microscopy, fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), drug loading, and release studies of curcumin‐loaded starches and SNP are carried out. The flow, swelling, and solubility of the native starches improve with nanoparticle fabrication. Setback viscosities of the starches are in the order PPnp < BAMnp < PP < BAM. The integrity of the starch granules is maintained after modification. The FTIR of loaded starches shows no interaction between the entities. Reduction in crystallinity of the SNP is observed in the XRD diffractogram. The drug loading efficiency of BAM, PP, BAMnp, and PPnp are 48.55 ± 0.11, 49.00 ± 0.11, 55.44 ± 0.40, and 54.72 ± 0.11, respectively. The drug release is improved when curcumin is loaded on the SNP. The release after 6 h is 33%, 25%, and 2% for BAMnp, PPnp, and pure curcumin, respectively. The properties of the SNP showed that they are potential candidates for the delivery of curcumin, a poorly water soluble drug.
Haemoblock hemostatic substance in reducing seroma formation post axillary lymph node dissection following breast conserving surgery
Authors: Isahak Mohamed Izzad , Abdullah Muhammad Safwan , Awang Raflis Ruzairee , Abdul Rashid Nor Faezan , Md Sikin Seniyah , Abdullah Suhaimi Shahrun Niza , Abdullah Norlia , Muhammad Rohaizak , Md Latar Nani Harlina 
Published: April 2024

AbstractBackgroundAxillary lymph node dissection (ALND) in breast cancer management, necessitates a nuanced understanding of complications that may impede treatment progression. This study scrutinize the impact of Haemoblock hemostatic solution, evaluation it's potential in reducing seroma complication by controlling lymph flow and obliterating axillary dead space.MethodA prospective, randomized, double‐blinded controlled trial was conducted with 58 patients undergoing breast conserving surgery (BCS) and ALND, stratified into two groups: Group A (ALND + Haemoblock, n = 29) and Group B (ALND + placebo, n = 29). Postoperative drainage charts were monitored, with the primary endpoint being the time to drain removal, Additionally, patients were observed for surgical site infection (SSI).ResultsGroup A exhibited a marginally higher mean total drain output (398 +/− 205 vs. 326 +/− 198) compared to Group B, this difference did not attain statistical significance (p = 0.176). Equally, the mean time to drain removal demonstrated no discernible distinction between the two groups (6 +/− 3.0 vs. 6 +/− 3.0, Group A vs. Group B, p = 0.526). During follow up, nine patients in Group A required seroma aspiration (mean aspiration 31 +/− 73) as compared to Group B, 6 patients required aspiration (mean aspiration 12 +/− 36), p = 0.222). No notable disparity in SSI rates between the groups was identified.ConclusionIn conclusion, the administration of Haemoblock did not manifest a discernible effect in mitigating seroma production, hastening drain removal, or influencing SSI rates following ALND. The study underscores the intricate and multifactorial nature of seroma formation, suggesting avenues for future research to explore combined interventions and protracted follow‐up periods for a more comprehensive understanding.
Carnivore guild utilization of hunter‐provided food sources in boreal forest
Authors: Wikenros Camilla , Nordli Kristoffer , Amato Giulia , Persson Jens , Ausilio Giorgia , Versluijs Erik , Eriksen Ane , Wabakken Petter , Aronsson Malin 
Published: April 2024

Scavenging is an alternative foraging strategy to predation for many carnivore species, as they shift between predation and scavenging in response to changes in resource availability. The use of carrion may lead to interspecific competition and is thus influenced by a risk–reward trade‐off to balance coexistence with guild‐members, where smaller species are expected to be more vigilant due to their vulnerability to larger competitors. We used cameras to investigate the utilization of viscera from the annual moose Alces alces hunt by four sympatric carnivore species: wolf Canis lupus, wolverine Gulo gulo, red fox Vulpes vulpes and pine marten Martes martes in south–central Scandinavia, in relation to body size and habitat. Red foxes had highest probability of visiting viscera sites in both open and forested habitats. Visits by both red foxes and pine martens were longer in open habitats, while number of visits or activity did not differ between habitats. For pine martens, the probability of visiting viscera sites was twice as high in forest compared to open habitat; consequently, red foxes showed the highest overall use of viscera. Red foxes were most vigilant, especially in open habitat, whereas wolverines and pine martens spent a higher proportion of time feeding. Increased vigilance of red foxes facilitates extended resources use in open habitat, while for pine martens, the risk–reward decision occurs before leaving forest cover and entering open habitats. Viscera were not used to a large extent by wolves or wolverines. Wolves are generally less prone to scavenging and wolverines probably use other food resources, more suitable for caching. Overall, competition did not prevent use of viscera, probably due to small‐scale temporal segregation and limited use by the larger carnivores. Consequently, this pulse of human‐subsidized food resources before winter may have important implications for the smaller facultative scavengers.Keywords: carrion use, interspecific competition, pine marten, red fox, wolf, wolverine
RNA‐binding proteins in pain
Authors: Smith Patrick R. , Campbell Zachary T. 
Published: April 2024

AbstractRNAs are meticulously controlled by proteins. Through direct and indirect associations, every facet in the brief life of an mRNA is subject to regulation. RNA‐binding proteins (RBPs) permeate biology. Here, we focus on their roles in pain. Chronic pain is among the largest challenges facing medicine and requires new strategies. Mounting pharmacologic and genetic evidence obtained in pre‐clinical models suggests fundamental roles for a broad array of RBPs. We describe their diverse roles that span RNA modification, splicing, stability, translation, and decay. Finally, we highlight opportunities to expand our understanding of regulatory interactions that contribute to pain signaling.This article is categorized under:
RNA Interactions with Proteins and Other Molecules > Protein‐RNA Interactions: Functional Implications
Translation > Regulation
RNA in Disease and Development > RNA in Disease