bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Ofloxacin in the Treatment of Laparoscopically Documented Acute Pelvic Inflammatory Disease (Salpingitis)
Authors: Peipert J. F. , Sweet R. L. , Walker C. K. , Kahn J. , Rielly-Gauvin K. 
Published: March 2007

Objective: To evaluate the safety and efficacy of intravenous and oral ofloxacin monotherapy in the treatment of laparoscopically documented acute pelvic inflammatory disease (PID).Methods: This study was conducted as an open‐label, phase‐III, uncontrolled, multicenter study. Patients identified with laparoscopic findings of salpingitis were treated with 400 mg of intravenous ofloxacin every 12 hours followed by 400 mg of oral ofloxacin every 12 hours for 10 to 14 days. Patients were evaluated five times for clinical and microbial efficacy. Since laparoscopy was performed only at admission, pathogens identified laparoscopically were presumed eradicated if they were present on the laparoscopic culture and the patient was clinically cured or improved at final evaluation.Results: Of the 70 patients evaluable for safety (intent‐to‐treat population), the mean age was 25.6 years. Sixty‐one of 70 patients (87%) were cured, one improved, one did not improve, and seven were unevaluable because they discontinued study participation. Fifty‐one were evaluable for clinical efficacy: 50 (98%) were cured and one did not improve. Sixteen were evaluable for expanded microbiological efficacy: three had documented Neisseria gonorrhoeae; 12, Chlamydia trachomatis; and one, a mixed infection of both organisms. All cervical, laparoscopic, and endometrial cultured pathogens, including N. gonorrhoeae and C. trachomatis, were eradicated or presumed eradicated at the posttherapy visit. No serious or unexpected adverse events occurred.Conclusions: Ofloxacin monotherapy was effective and well tolerated in the treatment of laparoscopically proven PID in a geographically diverse population. Future studies are necessary to evaluate long‐term outcomes and sequelae of PID treatment with single agent therapy. Infect. Dis. Obstet. Gynecol. 7:138–144, 1999.
Infertility Following Pelvic Inflammatory Disease
Authors: Pavletic A. J. , Wölner-Hanssen P. , Paavonen J. , Hawes S. E. , Eschenbach D. A. 
Published: March 2007

Objective: To assess the frequency of infertility after pelvic inflammatory disease (PID) and factors
important in postinfectious tubal damage in an urban population at high risk for sexually transmitted
diseases.Methods: From a cohort of 213 women with PID documented by laparoscopy and/or endometrial
biopsy, 58 women (27% of the initial cohort) were interviewed by phone 2 to 9 years after an index
episode of PID. Data regarding the initial history, physical examination, microbiology, laparoscopic,
and serologic findings, and data concerning interval contraception, subsequent pregnancy, subsequent
infection, and chronic pelvic pain were compared among those with and without infertility
at follow up.Results: Nineteen (40%) of the 48 women not using contraception were involuntarily infertile
after the index episode of PID. Compared with those who had an interval pregnancy, infertile
women were older (P = 0.02), more likely to have a history of infertility prior to the index episode
of PID (P = 0.001), and were more likely to have occluded or partially occluded fallopian tubes (P = 0.03), peritubal adhesions (P = 0.007), or perihepatic adhesions (P = 0.02) seen by laparoscopy
performed during the index episode. Surprisingly, recovery of Chlamydia trachomatis was negatively
related to infertility (P = 0.001), although a similar proportion of both groups had chlamydia
immunoglobulin M antibody (40% vs. 31%). Chlamydia heat shock protein was weakly related to
infertility (P = 0.08). The isolation of Neisseria gonorrhoeae was not significantly different between
groups (53% vs. 57%).Conclusions: The high rate of postinfection infertility found was probably related to a combination
of tubal damage before and during the index episode of PID. Prevention of recurrent PID and better
understanding of the pathophysiology of postinfection tubal damage (which may differ between
chlamydia and gonorrhea) is needed to develop more effective strategies to reduce permanent tubal
damage. Infect. Dis. Obstet. Gynecol. 7:145–152, 1999.
Semiquantitative Bacterial Observations With Group B Streptococcal Vulvovaginitis
Authors: Monif G. R. G. 
Published: March 2007

Objective: Group B streptococcal (GBS) vulvovaginitis is a poorly‐delineated clinical entity. The purpose of this study is to report semiquantitative data from four cases of GBS vulvovaginitis and to comment on their significance in terms of the in vitro inhibitory capabilities of GBS.Methodology: Four patients whose clinical presentations were consistent with GBS vulvovaginitis, from whom GBS was isolated and for whom semi‐quantitative as well as qualitative microbiologic data existed, were identified.Results: To produce vulvovaginitis, GBS must be at a high multiplicity (108
CFU/g of vaginal fluid). Single coisolates were identified in three of the four cases (two cases of Escherichia coli and one case of Staphylococcus aureus). Group B streptococcus does not inhibit either of these bacteria in vitro.Conclusion: When the growth requirements for the demonstration of in vitro inhibition for GBS or lack thereof are met in vivo, the in vivo observations are consistent with those projected from the in vitro data. Infect. Dis. Obstet. Gynecol. 7:227–229, 1999.
Human Papillomavirus Cervical Infection and Associated Risk Factors in a Region of Argentina With a High Incidence of Cervical Carcinoma
Authors: Tonon S. A. , Picconi M. A. , Zinovich J. B. , Liotta D. J. , Bos P. D. , Galuppo J. A. , Alonio L. V. , Ferreras J. A. , Teyssié A. R. 
Published: March 2007

Objective: To assess the prevalence and potential risk
factors associated with human papillomavirus (HPV) cervical infection among
women residing in a region of northeastern Argentina with a high incidence of
cervical cancer.Methods: A case‐control study of 330 women participating in a cervical
cytological screening program conducted in Posadas city, Misiones, Argentina,
from February 1997 to November 1998 was carried out. Standardized
questionnaires were administered, and clinical examination including
colposcopy was performed. Fresh endocervical specimens for HPV DNA
detection by generic polymerase chain reaction were collected and the products
typed by dot‐blot hybridization.Results: Human papillomavirus DNA was found in 61%
of samples analyzed (185/301). Samples with normal cytology had a 43% infection
rate (85/199), while those classified as low‐grade squamous intraepithelial lesion, high‐grade
squamous intraepithelial lesion, and invasive cervical carcinoma had an infection rate of 96%
(53/55), 100% (29/29), and 100% (18/18), respectively. Human papillomavirus typing showed
a 64% (118/185) prevalence of type 16 among all the infected population analyzed; type 16 was
detected among 49% (42/85) of infected samples with normal.cytology and in an average of
74% (74/100) with abnormal cytology. Sexual behavior, residence in southern Paraguay,
and history of a previous sexually transmitted diseases were the main risk factors associated
with high‐grade cervical lesions.Conclusions: An elevated prevalence of HPV infection was detected in this
population, which also has a high incidence of cervical cancer. The broad distribution of
high‐risk HPV type 16 in women with normal cytology and colposcopy suggests that viral
infection is an important determinant of regional cancer incidence. Infect. Dis. Obstet.
Gynecol. 7:237–243, 1999.
Undergraduate training in palliative medicine in Germany: what effect does a curriculum without compulsory palliative care have on medical students’ knowledge, skills and attitudes?
Authors: Ostgathe Christoph , Voltz Raymond , Nauck Friedemann , Klaschik Eberhard 
Published: March 2007

Introduction: The effect of a curriculum without compulsory courses in palliative medicine on students’ knowledge, kills and attitudes towards the care of dying patients and decisions at the end of life was investigated. Methods: In a cross sectional, questionnaire based survey at the medical school of Bonn 1st, 3rd and 5th year students had to grade their knowledge and skills in items concerning palliative medicine. Attitudes towards end of life decisions were queried. Results: The return rate was 78%. Significant increase in self estimation of certainty comparing 1st to 5th year students were detected for the items distinction between palliative and curative medicine (p <0.001), symptom control (p <0.001), pain management (p =0.001) and communication (p =0.036). No significant differences were recorded for the items accompaniment of dying patients, breaking bad news and integration of spiritual aspects. The low overall certainty is reflected in poor knowledge. A significant decrease of the approval for euthanasia by request was evident (p =0.012). Discussion: A medical curriculum without compulsory course does increase confidence in some of the core competencies in palliative care, but the overall results at the end of the training are poor. The WHO claims that palliative care has to be “compulsory in courses leading to a basic professional qualification” has still to be fulfilled in Germany. This study can serve as a baseline to evaluate the effect of mandatory courses in palliative care.
A Response to Tony Richie’s ‘Azusa Era Optimism: Bishop J.H. King’s Pentecostal Theology of Religions as a Possible Paradigm for Today’
Authors: Kärkkäinen Veli-Matti 
Published: March 2007

AbstractIn this appreciative response to Tony Richie’s paper, Richie is urged to relate the Pentecostal theology of religions of Bishop King to a wider theology of religions discourse and to be more specific about what he means by King’s alleged ‘optimism’ about religions.
Pneumatological Perspectives for A Theology of Nature: The Holy Spirit in Relation to Ecology and Technology
Authors: Gabriel Andrew 
Published: March 2007

AbstractHow does the relationship of the Holy Spirit to creation guide theology of nature? This paper will propose that, on a cosmological level, the Spirit, as the ‘Giver of Life’, produces kinship among creatures, that the Spirit suffers with creation’s suffering and as his perfecting work is frustrated, and that the Spirit gifts certain aspects of technological culture. The presence of the Spirit in nature causes us to respect nature, nevertheless, the Spirit may guide people to shape nature in ways that are consonant with the Spirit’s life-giving work.
Pentecostal Ecclesiology: A Methodological Proposal for A Diverse Movement
Authors: Clifton Shane 
Published: March 2007

AbstractThis paper is stimulated by the need to develop an ecclesiological method that is capable of describing and analysing the diverse self-understandings that characterize global Pentecostalism (or any Christian Church). It begins by observing the limitations of idealist approaches to ecclesiology, and instead proposes a concrete ecclesiological method. Concrete ecclesiology will include the narrative of particular Churches and movements, describe the explicit and implicit self-understanding that accompanies this narrative, and assess ecclesial transitions. Since the Church is a human and divine community, analysis will incorporate theological categories and the human sciences. Consequently, the paper considers how the ecclesiologist might appropriate the multifaceted discipline of sociology. The goal is an ecclesiology that is not reduced only to ideal categories, but that is capable of analysing the complex reality of indigenous Pentecostal Churches.
Lighting for people with dementia
Authors: Torrington J M , Tregenza P R 
Published: March 2007

Guidelines for the design of lighting in residential buildings used by people with dementia are derived from the results of two research projects and a literature review. General guidance on lighting for the older person is summarised and the special requirements of those living with dementia are examined under four headings: the diurnal cycle, a view of nature and the outside world, support for individual and social activities, and recognition of place. The aim is a visual environment that enhances physical well-being and enjoyment of life.
Reduced natural killer (NK) function associated with high-risk myelodysplastic syndrome (MDS) and reduced expression of activating NK receptors
Authors: Epling-Burnette Pearlie K. , Bai Fanqi , Painter Jeffrey S. , Rollison Dana E. , Salih Helmut R. , Krusch Matthias , Zou JianXiang , Ku Edna , Zhong Bin , Boulware David , Moscinski Lynn , Wei Sheng , Djeu Julie Y. , List Alan F. 
Published: March 2007

AbstractMyelodysplastic syndromes (MDS) are characterized by ineffective hematopoiesis with potential for progression to acute myeloid leukemia (AML). We compared natural killer (NK) cytolytic function in 48 MDS patients with 37 healthy donors and found reduced activity in the patient population (K562 cytolysis, 19% ± 21% SD versus 40% ± 17%) (P < .001). NK cytotoxicity in MDS patients was reduced against 3 disparate tumor targets with differential activating receptor requirement, suggesting global defects in NK function. Reduced NK function in MDS was significantly associated with higher International Prognostic Score (P = .01), abnormal karyotype (P = .05), the presence of excess blasts (P = .01), and age-adjusted bone marrow hypercellularity (P = .04). MDS patients had a display of the activating receptor NKp30, and NKG2D down-regulation closely correlated with impaired NK function (P = .001). NKG2D ligands (MICA and MICB) were expressed on CD34+ cells from bone marrow of 30% of MDS patients and a leukemic cell line derived from an MDS patient (MDS1). Collectively, these findings suggest that impairment of NK cytolytic function derives in part from reduced activating NK receptors such as NKG2D in association with disease progression. Evasion of NK immunosurveillance may have importance for MDS disease progression.
Long-term disease-free survivors with cytogenetically normal acute myeloid leukemia and MLL partial tandem duplication: a Cancer and Leukemia Group B study
Authors: Whitman Susan P. , Ruppert Amy S. , Marcucci Guido , Mrózek Krzysztof , Paschka Peter , Langer Christian , Baldus Claudia D. , Wen Jing , Vukosavljevic Tamara , Powell Bayard L. , Carroll Andrew J. , Kolitz Jonathan E. , Larson Richard A. , Caligiuri Michael A. , Bloomfield Clara D. 
Published: March 2007

AbstractThe clinical impact of MLL partial tandem duplication (MLL-PTD) was evaluated in 238 adults aged 18 to 59 years with cytogenetically normal (CN) de novo acute myeloid leukemia (AML) who were treated intensively on similar Cancer and Leukemia Group B protocols 9621 and 19808. Twenty-four (10.1%) patients harbored an MLL-PTD. Of those, 92% achieved complete remission (CR) compared with 83% of patients without MLL-PTD (P = .39). Neither overall survival nor disease-free survival significantly differed between the 2 groups (P = .67 and P = .55, respectively). Thirteen MLL-PTD+ patients relapsed within 1.4 years of achieving CR. MLL-PTD+ patients who relapsed more often had other adverse CN-AML–associated molecular markers. In contrast with previously reported studies, 9 (41%) MLL-PTD+ patients continue in long-term first remission (CR1; range, 2.5-7.7 years). Intensive consolidation therapy that included autologous peripheral stem-cell transplantation during CR1 may have contributed to the better outcome of this historically poor-prognosis group of CN-AML patients with MLL-PTD.
Postremission treatment of elderly patients with acute myeloid leukemia in first complete remission after intensive induction chemotherapy:results of the multicenter randomized Acute Leukemia French Association (ALFA) 9803 trial
Authors: Gardin Claude , Turlure Pascal , Fagot Thierry , Thomas Xavier , Terre Christine , Contentin Nathalie , Raffoux Emmanuel , de Botton Stephane , Pautas Cecile , Reman Oumedaly , Bourhis Jean-Henri , Fenaux Pierre , Castaigne Sylvie , Michallet Mauricette , Preudhomme Claude , de Revel Thierry , Bordessoule Dominique , Dombret Herve 
Published: March 2007

AbstractIn elderly patients with acute myeloid leukemia (AML) treated intensively, no best postremission strategy has emerged yet. This clinical trial enrolled 416 patients with AML aged 65 years or older who were considered eligible for standard intensive chemotherapy, with a first randomization comparing idarubicin with daunorubicin for all treatment sequences. After induction, an ambulatory postremission strategy based on 6 consolidation cycles administered monthly in outpatients was randomly compared with an intensive strategy with a single intensive consolidation course similar to induction. Complete remission (CR) rate was 57% with 10% induction deaths, and estimated overall survival was 27% at 2 years and 12% at 4 years, without notable differences between anthracycline arms. Among the 236 patients who reached CR, 164 (69%) were randomized for the postremission comparison. In these patients, the multivariate odds ratio in favor of the ambulatory arm was 1.51 for disease-free survival (P =.05) and 1.59 for overall survival from CR (P =.04). Despite repeated courses of chemotherapy associated with a longer time under treatment, the ambulatory arm was associated with significantly shorter rehospitalization duration and lower red blood cell unit and platelet transfusion requirements than observed in the intensive arm. In conclusion, more prolonged ambulatory treatment should be preferred to intensive chemotherapy as postremission therapy in elderly patients with AML reaching CR after standard intensive remission induction.
Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
Authors: Nguyen Vicky PKH , Chen Stephen H , Trinh Jason , Kim Harold , Coomber Brenda L , Dumont Daniel J 
Published: March 2007

Abstract

Background
The lymphatic system complements the blood circulatory system in absorption and transport of nutrients, and in the maintenance of homeostasis. Angiopoietins 1 and 2 (Ang1 and Ang2) are regulators of both angiogenesis and lymphangiogenesis through the Tek/Tie-2 receptor tyrosine kinase. The response of endothelial cells to stimulation with either Ang1 or Ang2 is thought to be dependent upon the origin of the endothelial cells. In this study, we examined the effects of the angiopoietins on lymphatic, venous and arterial primary endothelial cells (bmLEC, bmVEC and bmAEC, respectively), which were isolated and cultured from bovine mesenteric vessels.

Results
BmLEC, bmVEC and bmAEC cell populations all express Tie-2 and were shown to express the appropriate cellular markers Prox-1, VEGFR3, and Neuropilin-1 that define the particular origin of each preparation. We showed that while bmLECs responded slightly more readily to angiopoietin-2 (Ang2) stimulation, bmVECs and bmAECs were more sensitive to Ang1 stimulation. Furthermore, exposure of bmLECs to Ang2 induced marginally higher levels of proliferation and survival than did exposure to Ang1. However, exposure to Ang1 resulted in higher levels of migration in bmLECs than did to Ang2.

Conclusion
Our results suggest that although both Ang1 and Ang2 can activate the Tie-2 receptor in bmLECs, Ang1 and Ang2 may have distinct roles in mesenteric lymphatic endothelial cells.
Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro
Authors: Mamo Solomon , Gal Arpad Baji , Bodo Szilard , Dinnyes Andras 
Published: March 2007

Abstract
Background
Real-time PCR is an efficient tool to measure transcripts and provide valuable quantitative information on gene expression of preimplantation stage embryos. Finding valid reference genes for normalization is essential to interpret the real-time PCR results accurately, and understand the biological dynamics during early development. The use of reference genes also known as housekeeping genes is the most widely applied approach. However, the different genes are not systematically compared, and as a result there is no uniformity between studies in selecting the reference gene. The goals of this study were to compare a wide selection of the most commonly used housekeeping genes in mouse oocytes and preimplantation stage embryos produced under different culture conditions, and select the best stable genes for normalization of gene expression data.

Results
Quantitative real time PCR method was used to evaluate 12 commonly used housekeeping genes (Actb, Gapdh, H2afz, Hprt, Ppia, Ubc, Eef1e1, Tubb4, Hist2h2aa1, Tbp, Bmp7, Polr2a) in multiple individual embryos representing six different developmental stages. The results were analysed, and stable genes were selected using the geNorm software. The expression pattern was almost similar despite differences in the culture system; however, the transcript levels were affected by culture conditions. The genes have showed various stabilities, and have been ranked accordingly.

Conclusion
Compared to earlier studies with similar objectives, we used a unique approach in analysing larger number of genes, comparing embryo samples derived in vivo or in vitro, analysing the expression in the early and late maternal to zygote transition periods separately, and using multiple individual embryos. Based on detailed quantification, pattern analyses and using the geNorm application, we found Ppia, H2afz and Hprt1 genes to be the most stable across the different stages and culture conditions, while Actb, the classical housekeeping gene, showed the least stability. We recommend the use of the geometric averages of those three genes for normalization in mouse preimplantation-stage gene expression studies.
The molecular signature of therapeutic mesenchymal stem cells exposes the architecture of the hematopoietic stem cell niche synapse
Authors: Pedemonte Enrico , Benvenuto Federica , Casazza Simona , Mancardi Gianluigi , Oksenberg Jorge R , Uccelli Antonio , Baranzini Sergio E 
Published: March 2007

Abstract

Background
The hematopoietic stem cells (HSCs) niche of the bone marrow is comprised of HSCs, osteoblasts, endothelial cells and a stromal component of non-hematopoietic multipotent cells of mesenchymal origin named "mesenchymal stem cells" (MSCs).

Results
Here we studied the global transcriptional profile of murine MSCs with immuno-therapeutic potential and compared it with that of 486 publicly available microarray datasets from 12 other mouse tissues or cell types. Principal component analysis and hierarchical clustering identified a unique pattern of gene expression capable of distinctively classifying MSCs from other tissues and cells. We then performed an analysis aimed to identify absolute and relative abundance of transcripts in all cell types. We found that the set of transcripts uniquely expressed by MSCs is enriched in transcription factors and components of the Wnt signaling pathway. The analysis of differentially expressed genes also identified a set of genes specifically involved in the HSC niche and is complemented by functional studies that confirm the findings. Interestingly, some of these genes play a role in the maintenance of HSCs in a quiescent state supporting their survival and preventing them from proliferating and differentiating. We also show that MSCs modulate T cell functions in vitro and, upon in vivo administration, ameliorate experimental autoimmune encephalomyelitis (EAE).

Conclusion
Altogether, these findings provide novel and important insights on the mechanisms of T cell function regulation by MSCs and help to cement the rationale for their application in the treatment of autoimmune diseases.