bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term evolution reveals the role of the circadian cycle in the environmental adaptation of cyanobacteria
Authors: Mendaña Alfonso , Santos-Merino María , Gutiérrez-Lanza Raquel , Domínguez-Quintero Marina , Medina Juan Manuel , González-Guerra Ana , Campa Víctor , Ducos-Galand Magaly , López-Igual Rocío , Volke Daniel C. , Gugger Muriel , Nikel Pablo I. , Mazel Didier , de la Cruz Fernando , Fernández-López Raúl 
Published: March 2024

ABSTRACTCircadian clocks synchronize internal cellular states with diurnal rhythms. Widespread in bacteria and eukaryotes, they regulate a variety of physiological processes, from hormone secretion in animals to carbon fixation in photosynthetic organisms. The adaptive role of circadian clocks is assumed to stem from their ability to anticipate environmental change, yet their impact on ecological adaptation remains unclear. Here, we use experimental evolution to study the interplay between fitness and circadian regulation in the model cyanobacteriumSynechococcus elongatusPCC 7942. After 1,200 generations under continuous, high-intensity illumination, we obtained a strain that grew six times faster than its ancestral counterpart. Genome sequencing revealed three mutations fixed in the population, two of which replicated the fast-growing phenotype in the wild-type. A deletion in SasA, a key circadian regulator, was essential for fast growth. Transcriptomic and metabolomic analyses revealed that this mutation perturbed the rhythmicity of the cycle, while simultaneously locking the cell in a transcriptomic response to high intensity illumination. A comparison with another fast- growing isolate, UTEX 2973, showed convergent transcriptomic states despite different driving mutations. Our results indicate that the clock acts not only as a timekeeping device, but also as an adaptive mechanism to optimize growth across diverse ecological conditions.
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channel
Authors: Yadhanapudi Lalitha , Bhadra Pratiti , Barbieri Guido , Jung Martin , Lommel Mark , Helms Volkhard , Römisch Karin 
Published: March 2024

AbstractProtein secretion begins with protein translocation through the universally conserved Sec61 channel in the endoplasmic reticulum (ER) membrane. The function of its β-subunit, called Sbh1 in yeast, remains poorly understood. Sbh1/Sec61β has a cytosolic domain consisting of two parts: the N-terminal approximately 40 amino acids are intrinsically unstructured, followed by a conserved membrane proximal (CMP) domain. Sbh1 is anchored to the Sec61 channel by a single C-terminal transmembrane domain. Phosphorylation of the Sbh1/Sec61β intrinsically unstructured domain allows regulated import of specific proteins into the ER. We show here that S3/T5 phosphorylation of Sbh1 results in a conformational change in the Sbh1 intrinsically unstructured N-terminus. We also address the function of the Sbh1/Sec61β CMP domain. We identify a potential interaction site in the Sbh1 CMP domain with the Sec61 N-terminal amphipathic helix using molecular dynamics (MD) modelling. Peptide panning revealed that the Sbh1 CMP domain contains a binding site for Sbh1-dependent signal peptides. Using site-directed mutagenesis, we show that the CMP region controls import of Sbh1-dependent translocation substrates into the ER. We conclude that the Sbh1 CMP domain functions as a gatekeeper that recognizes and guides specific substrates towards the Sec61 channel for insertion.
Cortico-striatal action control inherent of opponent cognitive-motivational styles
Authors: Avila Cassandra , Sarter Martin 
Published: March 2024

AbstractTurning on cue or stopping at a red light requires attending to such cues to select action sequences, or suppress action, in accordance with learned cue-associated action rules. Cortico-striatal projections are an essential part of the brain’s attention-motor interface. Glutamate-sensing microelectrode arrays were used to measure glutamate transients in the dorsomedial striatum (DMS) of male and female rats walking a treadmill and executing cued turns and stops. Prelimbic-DMS projections were chemogenetically inhibited to determine their behavioral necessity and the cortico-striatal origin of cue-evoked glutamate transients. Furthermore, we investigated rats exhibiting preferably goal-directed (goal trackers, GTs) versus cue-driven attention (sign trackers, STs), to determine the impact of such cognitive-motivational biases on cortico-striatal control. GTs executed more cued turns and initiated such turns more slowly than STs. During turns, but not missed turns or cued stops, cue-evoked glutamate concentrations were higher in GTs than in STs. In STs, turn cue-locked glutamate concentrations frequently peaked twice or three times, contrasting with predominately single peaks in GTs. In GTs, but not STs, inhibition of prelimbic-DMS projections attenuated turn rates and turn cue-evoked glutamate concentrations and increased the number of turn cue-locked glutamate peaks. These findings indicate that turn cue-evoked glutamate release in GTs is tightly controlled by cortico-striatal neuronal activity. In contrast, in STs, glutamate release from DMS glutamatergic terminals may be regulated by other striatal circuitry, preferably mediating cued suppression of action and reward tracking. As cortico-striatal dysfunction has been hypothesized to contribute to a wide range of disorders, including complex movement control deficits in Parkinson’s disease and compulsive drug taking, the demonstration of phenotypic contrasts in cortico-striatal control implies the presence of individual vulnerabilities for such disorders.Significance StatementAdaptive behavior involves the selection of behaviorally significant cues and the capacity of selected cues to control behavioral action. Neuronal projections from cortex to striatum are essential for such an integration of attentional with motor functions. Here we demonstrated that glutamate release from cortico-striatal projections primarily influences cued turns but not cued suppression of actions (cued stops). Cortico-striatal control of cued turning was especially powerful in rats which, as a psychological trait, preferably deploy goal-directed attention. Together, our findings demonstrate the role of cortico-striatal input in cued action selection, and they emphasize the experimental and biopsychological significance of investigating the brain’s attentional-motor interface in the context of broader individual differences in cognitive-motivational styles.
Independent expansion, selection and hypervariability of theTBC1D3gene family in humans
Authors: Guitart Xavi , Porubsky David , Yoo DongAhn , Dougherty Max L. , Dishuck Philip C. , Munson Katherine M. , Lewis Alexandra P. , Hoekzema Kendra , Knuth Jordan , Chang Stephen , Pastinen Tomi , Eichler Evan E. 
Published: March 2024

ABSTRACTTBC1D3is a primate-specific gene family that has expanded in the human lineage and has been implicated in neuronal progenitor proliferation and expansion of the frontal cortex. The gene family and its expression have been challenging to investigate because it is embedded in high-identity and highly variable segmental duplications. We sequenced and assembled the gene family using long-read sequencing data from 34 humans and 11 nonhuman primate species. Our analysis shows that this particular gene family has independently duplicated in at least five primate lineages, and the duplicated loci are enriched at sites of large-scale chromosomal rearrangements on chromosome 17. We find that most humans vary along twoTBC1D3clusters where human haplotypes are highly variable in copy number, differing by as many as 20 copies, and structure (structural heterozygosity 90%). We also show evidence of positive selection, as well as a significant change in the predicted human TBC1D3 protein sequence. Lastly, we find that, despite multiple duplications, humanTBC1D3expression is limited to a subset of copies and, most notably, from a single paralog group:TBC1D3-CDKL. These observations may help explain why a gene potentially important in cortical development can be so variable in the human population.
N-glycosylated molecules act as a co-precipitant in RNA purification
Authors: Kim Sungchul , Choi Yong-geun , Janssen Kirsten , Büll Christian , Joshi Bhagyashree S. , Pomorski Adam , Raz Vered , Tanenbaum Marvin E. , Miesen Pascal , Li Zeshi , Joo Chirlmin 
Published: March 2024

SummaryA recent ground-breaking study suggested that small RNA from mammalian cells can undergo N-glycan modifications (termed glycoRNA)1. The discovery relied upon a metabolic glycan labeling strategy in combination with commonly used phase-separation-based RNA isolation. Following the reported procedure, we likewise identified an N-glycosylated species in the RNA fraction. However, our results suggest that the reported RNase sensitivity of the glycosylated species depends on the specific RNA purification method. This suggests the possibility of co-purifying unexpected RNase-insensitive N-glycoconjugates during glycoRNA isolation, hinting at the complex biochemical nature of glycoRNA. Our study underscores the need for further research to elucidate the structural and biochemical properties of glycoRNA.
The histone chaperone CAF-1 prevents Rad52-mediated instability of the budding yeast ribosomal DNA during replication-coupled DNA double-strand break repair
Authors: Futami Hajime , Yamaji Tsugumi , Katayama Yuko , Arata Nanase , Kobayashi Takehiko , Sasaki Mariko 
Published: March 2024

ABSTRACTDNA replication-coupled chromatin assembly is crucial to maintain genome integrity. Here, we demonstrate that absence of the budding yeast histone chaperone CAF-1 induces chromosomal ribosomal RNA gene (rDNA) copy number changes as well as the production of extrachromosomal rDNA circles in a manner dependent on Fob1 that causes replication fork arrest in the rDNA, the homologous recombination protein Rad52, and its interaction with Proliferating Cell Nuclear Antigen. CAF-1 deficiency enhances transcription from the regulatory promoter E-pro, contributing to rDNA instability, but it also stabilizes the rDNA independently through its regulation of E-pro. Absence of CAF-1 induces end resection of DNA double-strand breaks (DSBs) formed at arrested replication forks, which are repaired in a manner dependent on the Mre11-Rad50-Xrs2 complex. CAF-1 deficiency causes partial defects in lagging strand synthesis coupled to nucleosome spacing in the rDNA. Our findings suggest that CAF-1 suppresses Rad52-mediated rDNA instability during repair of replication-coupled DSBs.
Do imprinting effects on CEOs affect tourism and hospitality enterprises’ corporate innovation?
Authors: Shan Yawen , Shi Da , Xu Shi 
Published: March 2024

Purpose
Based on imprinting theory and episodic future thinking, this paper aims to study how CEOs’ attributes and experiences inform innovation in tourism and hospitality businesses. It also explores ways to quantify innovation in this sector.

Design/methodology/approach
The authors quantitatively analysed innovation in tourism and hospitality using extensive data from companies’ annual reports. They further adopted multivariate regression to test how CEOs’ experience affects enterprise innovation.

Findings
Results demonstrate that CEOs’ academic education and rich work experience can promote corporate innovation. The authors also identified a mediating role of the tone of narrative disclosure in annual reports between CEOs’ academic education and corporate innovation. The imprinting effects of career experience and educational experience appear both independent and interactive.

Research limitations/implications
CEOs are more inclined to engage in corporate innovation when influenced by the combined imprinting effects of strategic management training and work experience. Additionally, leaders should consider how communication styles indirectly influence innovation activities.

Originality/value
This paper introduces an integrated perspective that blends imprinting theory and episodic future thinking to bridge knowledge gaps regarding the interaction of CEOs’ past experiences. This work enhances understanding of how CEOs’ imprinted experiences, together with their capacity for envisioning future scenarios, can drive corporate innovation.
Integrating mobile parcel lockers into last-mile delivery networks: an operational design for home delivery, stationary, and mobile parcel lockers
Authors: Peppel Marcel , Spinler Stefan , Winkenbach Matthias 
Published: March 2024

PurposeThe e-commerce boom presents new challenges for last-mile delivery (LMD), which may be mitigated by new delivery technologies. This paper evaluates the impact of mobile parcel lockers (MPL) on costs and CO2 equivalent (CO2e) emissions in existing LMD networks, which include home delivery and shipments to stationary parcel lockers.Design/methodology/approachTo describe customers’ preferences, we design a multinomial logit model based on recipients’ travel distance to pick-up locations and availability at home. Based on route cost estimation, we define the operating costs for MPLs. We devise a mathematical model with binary decision variables to optimize the location of MPLs.Findings Our study demonstrates that integrating MPLs leads to additional cost savings of 8.7% and extra CO2e emissions savings of up to 5.4%. Our analysis of several regional clusters suggests that MPLs yield benefits in highly populous cities but may result in additional emissions in more rural areas where recipients drive longer distances to pick-ups.Originality/valueThis paper designs a suitable operating model for MPLs and demonstrates environmental and economic savings. Moreover, it adds recipients’ availability at home to receive parcels improving the accuracy of stochastic demand. In addition, MPLs are evaluated in the context of several regional clusters ranging from large cities to rural areas. Thus, we provide managerial guidance to logistics service providers how and where to deploy MPLs.
Income-shifting arrangements of US multinational corporations and future stock price crash risk
Authors: Richardson Grant , Taylor Grantley , Hasan Mostafa 
Published: March 2024

PurposeThis study examines the importance of income income-shifting arrangements of US multinational corporations (MNCs) on future stock price crash risk.Design/methodology/approachThis study employs a sample of 7,641 corporation-year observations over the 2005–2017 period and uses ordinary least squares regression analysis.FindingsThe authors find that the income-shifting arrangements of MNCs are positively and significantly associated with stock price crash risk after controlling for corporate tax avoidance and other known determinants of stock price crash risk in the regression model. This result is robust to alternative measures of stock price crash risk and income-shifting, and several endogeneity tests. The authors also observe that income-shifting arrangements increase stock price crash risk both directly and indirectly through the information opacity channel. Finally, in cross-sectional analyses, the authors find that the positive association between income-shifting and stock price crash risk is more pronounced for MNCs that use tax haven subsidiaries and have weak corporate governance mechanisms.Originality/valueThe authors provide new empirical evidence that MNCs will likely face significant capital market consequences regarding their income-shifting arrangements.
Innovative leadership and sustainable performance: a moderation study through personality traits
Authors: Aman-Ullah Attia , Aziz Azelin , Mehmood Waqas , Vafin Aidar , Hassan Mohammad 
Published: March 2024

PurposeThe present study aims to investigate the relationship between innovative leadership and sustainable performance in the education sector. The present study also tested the moderation role of personality traits agreeableness, extraversion, emotional stability, conscientiousness and openness in the relationship.Design/methodology/approachData for the present study were collected from 209 university teachers. The employed sampling technique was convenience, and the sample size was calculated through the Kerjis–Morgan method. Furthermore, a survey method using a questionnaire was used in this study. For the data analysis, SPSS and SmartPLS were used.FindingsThe present study found that innovative leadership has a significantly positive relationship with sustainable performance. Results also confirmed the moderating effects of personality traits such as agreeableness, extraversion, emotional stability, conscientiousness and openness.Originality/valueThe relationship between innovative leadership and sustainable performance for the first time in the education sector’s context. Secondly, this study contributed to the moderating role of personality traits such as agreeableness, extraversion, emotional stability, conscientiousness and openness between innovative leadership and sustainable performance, which was a yet-to-explored phenomenon. The study model was tested through the combination of the big five-factor model and the theory of planned behaviour, which is another novelty of the study.
The role of trust and perceived risk on Muslim behavior in buying halal-certified food
Authors: Usman Hardius , Projo Nucke Widowati Kusumo , Chairy Chairy , Haque Marissa Grace 
Published: March 2024

Purpose
The purpose of this study to examine the factors that encourage/inhibit Muslim behavior in buying halal-certified food (HCF), based on two theories, the knowledge-attitude-behavior model and the attitude-behavior-context model; and study the impact of trust and perceived risk on Muslim behavior in buying HCF, and their role in moderating the relationship between halal awareness and religious commitment with Muslim behavior in buying HCF.

Design/methodology/approach
The research population target is Muslims aged 18 years or older who reside in Greater Jakarta and have purchased certified halal food at least once in the past month. The survey method is a self-administered survey using a purposive sampling technique. The online survey has been successful in getting 283 Muslim respondents. In analyzing the causal relationship and hypothesis testing, this research uses the partial least square – structural equation model.

Findings
This study reveals several results: attitude, halal awareness, religious commitment, trust and perceived risk have a significant influence on the frequency of Muslims buying HCF. Attitude mediates the impact of halal awareness, religious commitment and trust on the frequency of Muslims buying HCF; perceived risk and trust moderate the relationship between religious commitment and the frequency of Muslims buying HCF.

Originality/value
Research on halal food is still limited, including in Indonesia. Meanwhile, the study explores the actual behavior of consumers, particularly in a certified halal food context, which is still rare in the existing literature. At the same time, the intention-behavior gap can lead to wrong decisions. Furthermore, this study also studies how Muslims feel when they consume foods that are not certified as halal. Research like this has an immense opportunity to be developed because not many have been developed.
Effects of artisanal gold mining and routes towards sustainable development for a low‐profile mining community in Ghana
Authors: Mensah Justice , Amoah John Oti , Nketsiah‐Essoun Andrew 
Published: March 2024

AbstractThe objective of the study is to examine the effects of artisanal and small‐scale gold mining (ASGM) and routes towards sustainable development (SD) for a low‐profile mining community in Ghana. The study drew on the literature on mining, sustainable development, and the Natural Resource Curse (NRC) theory. Data were gathered using semi‐structured interviews and focus group discussions with 203 participants selected through the purposive and snowball sampling methods. The data were analysed using the thematic approach. The findings indicate that although ASGM supported livelihoods through employment and income generation, it had numerous negative effects that weakened the ‘pillars’ of SD, namely social, economic, and environmental sustainability. Among others, the study reveals conflicts, drop‐out and absenteeism among students and pupils, drug abuse, teenage pregnancies, child labour, and violence in the host community. Economically, ASGM affected agricultural production and food security following the destruction of farms and farmlands, while labour and capital were moved from farming to mining. Environmentally, air and water bodies were polluted, flora and fauna destroyed, and productive lands degraded. In short, the SD costs were perceived to be greater than the benefits, lending credence to the NRC theory. Several of these adverse effects were due to the fact that the activities were neither formalized nor regulated. Besides, the operations were characterized by inappropriate technology and lack of geological data, leading to unsustainable mining practices resulting in anti‐development outcomes. By way of routes towards sustainability, it is recommended that the operations be formalized under the existing Community Mining Scheme and regulated to ensure responsible, accountable, and sustainable mining. The Scheme should be strengthened in order to drive positive impact‐making mining operations by means of introducing a sustainability‐oriented licensing regime, sustainable mining technologies, access to land and geological data, stakeholder participation, capacity building on sustainable mining literacy, and eco‐friendly mine closure strategies.
Paraganglioma of the thyroid gland: A case report and literature review
Authors: Lee Yeuk‐Nam , Tsang Yi‐Po , Yuen Ka‐Wan Karen , Choi Chi‐Yee 
Published: March 2024

AbstractParaganglioma of the thyroid gland is a rare disease entity, with only a handful of cases documented in the literature. We report on a middle‐aged man with thyroid paraganglioma that presented as an incidental finding on the computed tomography scan of the thorax. Preoperative fine‐needle aspiration cytology (FNAC) was suggestive of a neuroendocrine tumour. Hemithyroidectomy was performed and histological findings were most compatible with paraganglioma arising from the thyroid gland. The diagnosis of primary thyroid paraganglioma is rarely established by FNAC preoperatively due to architectural similarities with other thyroid tumours; the most important clinical technique for differentiating thyroid paraganglioma from other histology is immunohistochemistry. According to the literature, the first‐line treatment for primary thyroid paraganglioma is surgical resection. The prognosis following surgical resection is generally good.