bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E2 levels of human synovial cells
Authors: Leizer Tali , Clarris Barry J. , Ash Patricia E. , Damme Joseph Van , Saklatvala Jeremy , Hamilton John A. 
Published: February 2007

AbstractMonocyte—macrophage polypeptides (monokines) cause synovial cells to increase the levels of putative mediators of destruction and inflammation. This interaction may account for some of the properties of rheumatoid pannus. We report here that samples of purified human interleukin‐1β(IL‐1β) and recombinant IL‐1β stimulate both the plasminogen activator activity and prostaglandin E2 levels of human synovial fibroblast‐like cells. The same holds true for purified pig IL‐1 (catabolin) and recombinant murine IL‐1. The elevation in plasminogen activator activity was inhibited by indomethacin, and this suggests that endogenous prostanoids are important in the IL‐1‐mediated stimulation of proteinase activity.
Articular indices of joint inflammation in rheumatoid arthritis. Correlation with the acute‐phase response
Authors: Thompson Paul W. , Silman Alan J. , Kirwan John R. , Currey Harry L. F. 
Published: February 2007

AbstractThe joints of 30 rheumatoid arthritis patients were assessed by one observer for signs of inflammation. Computer analysis was then used to calculate 70 different articular indices for each patient. Pearson correlation coefficients were calculated between serum C‐reactive protein levels and the articular indices. The results show that: 1) findings in a restricted set of examined joints were equivalent to those in a more complete set; 2) the simultaneous presence of joint tenderness and swelling yielded higher correlation than did either variable alone; and 3) joint ‘weighting’ for size yielded higher correlation than did simple counts.
Magnetic resonance imaging in the evaluation of patients with rheumatoid arthritis and subluxations of the cervical spine
Authors: Breedveld Ferdinand C. , Algra Paul R. , Vielvoye C. Jan , Cats Arnold 
Published: February 2007

AbstractWe used magnetic resonance imaging (MRI) to examine 21 patients with rheumatoid arthritis and vertebral subluxations of the cervical spine, in whom neurologic symptoms and signs indicated spinal cord compression. Based on neurologic signs, the patients were assigned to 1 of 3 classes: class I, no objective signs of cervical myelopathy (9 patients); class II, only 1 objective sign of cervical myelopathy (4 patients); or class III, 2 or more objective signs of cervical myelopathy (8 patients). Atlantoaxial subluxation (20 patients) and subluxations below C2 (6 patients) were detected equally well by MRI and radiography. MRI revealed physical distortion of the spinal cord in all class III patients with compressive myelopathy. This distortion was found less frequently in class II and class I patients (3 patients), and the difference was statistically significant (P < 0.005, class III versus class I and class II). No correlation was found between the vertebral dislocation (measured in millimeters) on plain radiographs and the presence of cord distortion on MRI. Myelography in class III patients showed that passage of contrast medium was blocked at the same level as the cord distortion seen on MRI. These findings suggest that MRI can serve as a useful, noninvasive procedure in the diagnosis and management of rheumatoid arthritis patients in whom compressive cervical myelopathy is suspected.
Myoadenylate deaminase deficiency and forearm ischemic exercise testing
Authors: Valen Peter A. , Nakayama Denny A. , Veum Judith , Sulaiman A. R. , Wortmann Robert L. 
Published: February 2007

AbstractMyoadenylate deaminase (MADA) deficiency has been associated with symptoms of postexertional aches, cramps, weakness, and skeletal muscle dysfunction. Measurement of plasma lactate and ammonia concentrations after forearm ischemic exercise has been suggested as a screening test for this disorder. We performed forearm ischemic tests on 3 patients with histochemically defined MADA deficiency and 13 healthy control subjects, in a standardized fashion. Our results demonstrated that subject effort and/or performance during the exercise portion of testing is a critical variable. In addition to lactate and ammonia, plasma purine compounds (adenosine, inosine, and hypoxanthine) were measured. The finding of decreased purine release after exercise in MADA‐deficient patients compared with that in normal individuals increases the specificity of the test and supports the hypothesis that disordered purine metabolism occurs in MADA deficiency.
The immunologic relationship of human neutrophil and skin collagenases
Authors: Hasty Karen A. , Stricklin George P. , Hibbs Margaret S. , Mainardi Carlo L. , Kang Andrew H. 
Published: February 2007

AbstractAn understanding of the immunologic relationships between collagenases of various cellular origins is necessary to define the roles of various cell types in the pathologic tissue destruction seen in chronic inflammatory diseases, such as rheumatoid arthritis. We compared the immunologic cross‐reactivity of human neutrophil and skin fibroblast collagenases, utilizing polyclonal antisera prepared to purified enzymes. Polyclonal antisera from rabbits immunized with neutrophil collagenase recognized fibroblast collagenase, as well as the neutrophil enzyme, when analyzed by immunoblot techniques. The cross‐reactive epitopes constituted a major proportion of the antibody population, as shown by competitive inhibition enzyme‐linked immunosorbent assay; 50% of the antibody to neutrophil collagenase was inhibited by skin collagenase. Paradoxically, antisera to fibroblast collagenase failed to recognize the neutrophil enzyme, either by immunoblot techniques or competitive inhibition enzyme‐linked immunosorbent assay, an observation which supports the notion that there are unique immunodominant epitopes. The cross‐reactivity with skin fibroblast collagenase shown by the neutrophil antibody suggests a conservation of epitopes between collagenases of different cellular origins. The presence of epitopes unique for each enzyme, however, could lead to a feasible approach for investigating the differential contribution of various cell types to collagenolytic activity in inflamed tissues.
Persistent synovial lymphocyte responses to cytomegalovirus antigen in some patients with rheumatoid arthritis
Authors: Ford Denys K. , Roza Doreen M. Da , Schulzer Michael , Reid Graham D. , Denegri Jorge F. 
Published: February 2007

AbstractSynovial lymphocytes from 6 of 40 patients with rheumatoid arthritis responded to cytomegalovirus antigen stimulation. 3H‐thymidine uptakes were more than 3 times greater than were those of the responses to 13 other microbial antigens. Similar results were obtained in 1 patient on 7 occasions over 17 months, and in the 5 other patients on each of 2 occasions. In 3 of the 6 patients, synovial lymphocyte responses to cytomegalovirus antigen were markedly different from simultaneous peripheral blood lymphocyte responses.
Monoclonal autoantibody from a (new zealand black x new zealand white)F1 mouse and some human scleroderma sera target an MTr 34,000 nucleolar protein of the U3 RNP particle
Authors: Reimer G. , Pollard K. M. , Penning C. A. , Ochs R. L. , Lischwe M. A. , Busch H. , Tan E. M. 
Published: February 2007

AbstractA monoclonal IgG2a antinucleolar autoantibody (72B9) was obtained by fusion of spleen cells from a (New Zealand black x New Zealand white)F1 mouse with myeloma cells (P3x63Ag8.653). Antibody 72B9 recognized a highly conserved nucleolar antigen present in both animal and plant cells. The staining pattern produced by antibody 72B9 in different cell substrates was identical with those obtained by scleroderma antibodies reactive with a basic (pi 8.5) nucleolar protein of Mr 34,000, which is associated with the U3 RNP particle. Western blotting further confirmed its reactivity with this scleroderma‐related U3 RNP protein.
Reaction of antipolynucleotide antibody from systemic lupus erythematosus patient serum with double‐stranded DNA complexed to protein
Authors: Faiferman Isidore , Koffler David 
Published: February 2007

AbstractAntipolynucleotide antibody from systemic lupus erythematosus patient serum was found to interact with common determinants of double‐stranded DNA (dsDNA) complexed to protein within nucleosomes, and with protein‐free denatured DNA. In contrast, purified dsDNA isolated from nucleosomes manifested decreased reactivity with antipolynucleotide antibody. These data suggest that immunoassays using denatured DNA antigen substrate are useful for identifying a broad spectrum of antipolynucleotide antibodies that are reactive with dsDNA complexed to protein.
Inhibition of neutrophil‐mediated degradation of isolated basement membrane collagen by nonsteroidal antiinflammatory drugs that inhibit degranulation
Authors: Neal Teresa M. , Vissers Margret C. M. , Winterbourn Christine C. 
Published: February 2007

AbstractThe ability of nonsteroidal antiinflammatory drugs to inhibit neutrophil‐mediated degradation of type IV collagen in an in vitro tissue injury model using glomerular basement membrane (GBM) containing immune complexes was investigated. Auranofin (2.5–10 μM), phenylbutazone (50–250 μM), sulfasalazine (250–1,000 μM), and 4‐bromophenacyl bromide (5–20 μM) each inhibited up to 70% of the collagen degradation, in parallel with almost complete inhibition of the release of azurophil and specific granule enzymes. These drugs had much less an effect on gelatinase release. Indomethacin and the antimalarials, which inhibited the neutrophil oxidative burst but not degranulation, had little effect on GBM collagen degradation. Our results do not necessarily imply that inhibition of neutrophil‐mediated degradation of connective tissue is relevant to the action of nonsteroidal antiinflammatory drugs in vivo; however, using the GBM model system, we have shown that when a drug inhibits granule enzyme release, there is an associated decrease in collagen degradation, whereas inhibition of the oxidative burst has relatively little effect.
Computer‐based patient education for older persons with osteoarthritis
Authors: Rippey Robert M. , Bill Dee , Abeles Micha , Day John , Downing Deborah S. , Pfeiffer Carol A. , Thal Sara E. , Wetstone Scott L. 
Published: February 2007

AbstractA program of 8 lessons on various aspects of osteoarthritis (OA), its treatment, and patient self‐care was prepared on an Apple IIc computer. The courses were then field‐tested and evaluated by 72 older (age range 52–88) OA patients in community centers for senior citizens. Statistical analysis of the findings showed significant increases in knowledge and significant self‐reported, beneficial behavior changes, including increased exercise, use of heat, and rest. Our findings demonstrate that older persons can use the computer to learn to cope with OA when a thoughtfully planned program is made available in a community setting.
Medical management of pneumococcal arthritis involving a knee prosthesis
Authors: Sublett Kevin L. , Katz Arnold L. 
Published: February 2007

AbstractSeptic arthritis involving prosthetic joints has been frequently reported, with Staphylococcus species being the pathogen most commonly noted. We report a case of septic arthritis of a prosthetic knee caused by Pneumococcus, a previously unreported pathogen in this condition, which uniquely responded to treatment with antibiotics.
Wegener's granulomatosis presenting with erosive arthritis
Authors: Jacobs Robert P. , Moore Marianne , Brower Anne 
Published: February 2007

AbstractWe describe a patient with Wegener's granulomatosis who presented with erosive arthritis. The patient's multisystem disease responded to cyclophosphamide treatment, and the erosions healed. Our findings suggest that Wegener's granulomatosis should be added to the differential diagnosis of diseases associated with erosive arthritis.
Complement C3b receptors on erythrocytes in patients with juvenile rheumatoid arthritis
Authors: Thomsen Bjarne S. , Heilmann Carsten , Jacobsen Svend E. H. , Pedersen Freddy K. , Morling Niels , Jakobsen Bodil K. , Svejgaard Arne , Nielsen Henrik 
Published: February 2007

AbstractForty patients with juvenile rheumatoid arthritis (JRA) were examined for C3b receptor (CR1) levels on erythrocytes (by an enzyme‐linked immunosorbent assay), levels of circulating immune complexes (IC) (by a polyethylene glycol precipitation–complement consumption method), C3d split products (by intermediate gel rocket immunoelectrophoresis), and HLA–A, B, and C (by microdroplet lymphocytotoxicity test). Lower CR1 levels were found predominantly among patients with JRA (mean 57%) compared with 40 age‐matched controls (mean 68%) (P = 0.008). The CR1 levels differed when the JRA patients were grouped by mode of disease onset and by clinical state at the time of testing (levels in patients with pauciarticular disease were higher than those in patients with polyarticular disease, and levels in patients with polyarticular disease were higher than those in patients with systemic disease) or by sex (girls had higher levels than boys; P = 0.01). The levels of circulating IC and C3d were elevated in 25% and 40% of JRA patients, respectively, and were mutually correlated (P < 0.05, τ = 0.20). A negative correlation was found between levels of C3d and the numbers of CR1 (P < 0.01, τ = ‐0.28), but concentrations of circulating IC did not correlate with CR1 values. CR1 levels were the same in 6 HLA–B27 positive and 25 HLA–B27 negative patients. These findings do not represent conclusive evidence that the number of CR1 on erythrocytes serves as a predictor of disease severity or as an indicator of disease activity in patients with JRA.
2,000‐Centigray total lymphoid irradiation for refractory rheumatoid arthritis
Authors: Trentham David E. , Belli James A. , Bloomer William D. , Anderson Ronald J. , Lane Heather , Reinherz Ellis L. , Austen K. Frank 
Published: February 2007

AbstractBecause toxicity with the use of 3,000 centiGray (cGy) of total lymphoid irradiation (TLI) was observed in an earlier study, 2,000‐cGy treatments were delivered in a 2‐portal format to 7 patients and in a modified 3‐portal fashion to 6 patients, as part of a randomized, investigator‐blinded trial of TLI treatment for refractory rheumatoid arthritis. Analysis of combined data from the 13 patients revealed statistically significant improvement in 5 clinical indicators of disease activity at the end of TLI and 6 and 12 months later, accompanied by T4‐specific immunosuppression. Management considerations resulted in the introduction of prednisone therapy in 5 patients, methotrexate in 4, and azathioprine in 1 during the interval of 8–12 months post‐TLI. Herpes zoster occurred in 5 patients prior to the initiation of this additional therapy. These data indicate that, in patients with rheumatoid arthritis, a TLI dose of 2,000 cGy is sufficient to produce measurable benefit that lasts for 6 months, and that the improvement can be maintained at 12 months by the use of prednisone and methotrexate.
Meclofenamate sodium monohydrate inhibits chemotactic factor–induced human polymorphonuclear leukocyte function. a possible explanation for its antiinflammatory effect
Authors: Perez H. Daniel , Elfman Fred , Marder Shelley 
Published: February 2007

AbstractMeclofenamate sodium monohydrate (MSM), a potent nonsteroidal antiinflammatory agent, specifically inhibits chemotactic factor‐induced human polymorphonuclear leukocyte functions: chemotaxis, degranulation, and generation of superoxide anion radicals. These effects of MSM were found to be dependent upon the concentrations of drug not bound to albumin (free drug), and were caused by its ability to interfere at both a receptor and post‐receptor (i.e., a step distal to mobilization of polymorphonuclear leukocyte intracellular Ca2+) level. These unique actions of MSM may provide a partial explanation for its potent antiinflammatory effect.
Felty's syndrome: long‐term followup after treatment with auranofin
Authors: Bellelli Anna , Veneziani Maura , Tumiati Bruno 
Published: February 2007

AbstractFive patients who had Felty's syndrome were treated with auranofin, 6 mg/day, for a period that ranged from 4 months to 2 years. All patients experienced both an improvement in articular symptoms and a normalization of the leukocyte count. Auranofin appears to be an effective treatment for Felty's syndrome, and to have a lower degree of toxicity than parenteral gold.
Increase in okm1 + granular lymphocytes in patients with rheumatoid arthritis
Authors: Cooper Sheldon M. , Roessner Karen , Ferriss John A. , Baigent Gregory , Bakke Antony C. 
Published: February 2007

AbstractPatients with very active rheumatoid arthritis that was being treated only with nonsteroidal antiinflammatory drugs had increased numbers of peripheral blood OKM1 + lymphocytes. In 3 patients, 90° light scatter analysis revealed a double lymphocyte peak. When sorted, the high scatter peak contained a large percentage of granular lymphocytes. Patients with mild‐to‐moderately active rheumatoid arthritis had normal levels of OKM1 + lymphocytes, but when the drugs were discontinued, the activity of the disease and the numbers of OKM1 + cells increased. Administration of piroxicam was associated with clinical improvement and a decrease in levels of OKM1 + cells. OKM1 + granular lymphocytes are increased in some rheumatoid arthritis patients, and their numbers may correlate with clinical disease activity and/or therapy.
Avidity of anti‐yersinia antibodies in yersiniosis patients with and without yersinia‐triggered reactive arthritis
Authors: Lahesmaa‐Rantala Riitta , Lehtonen Olli‐Pekka , Granfors Kaisa , Toivanen Auli 
Published: February 2007

AbstractAvidity of IgM, IgG, and IgA class anti‐Yersinia antibodies was compared in sera of 22 patients with yersiniosis and subsequent reactive arthritis versus sera of 22 patients without postinfection complications. An enzyme‐linked immunosorbent assay was used for antibody determination. Less than 2 months after onset of the infection, the patients with arthritis had fewer high‐avidity IgM antibodies against the bacterial lipopolysaccharide (P = 0.035) and more high‐avidity IgA antibodies against bacterial cell extract (P = 0.039) than did the patients without arthritis. This difference increased with time.
Subclinical lung inflammation in primary sjögren's syndrome. relationship between bronchoalveolar lavage cellular analysis findings and characteristics of the disease
Authors: Hatron Pierre‐Yves , Wallaert Benoît , Gosset Didier , Tonnel Andréa‐Bernard , Gosselin Bernard , Voisin Cyr , Devulder Bernard 
Published: February 2007

AbstractTo directly evaluate whether a subclinical alveolar inflammation is associated with primary Sjögren's syndrome (SS), we evaluated the distribution of cells obtained by bronchoalveolar lavage (BAL) from the lower respiratory tract in 29 patients who had primary SS, but who were free of clinical pulmonary symptoms and had normal findings on chest roentgenograms. There was no difference in total cell counts of specimens from patients versus those of controls. An abnormal differential cell count was noted in 16 patients (55%). Two patterns of alveolitis were observed: a pure lymphocyte alveolitis (>18% lymphocytes, present in 11 patients) and a neutrophil alveolitis (>4% neutrophils, present in 5 patients). There was associated lymphocytosis in 4 of the patients with neutrophil alveolitis. All patients had normal results on pulmonary function tests. Patients with abnormal BAL findings showed clinical and biologic indexes of more severe disease than did those with normal BAL results, as demonstrated by greater extraglandular extension of the disease, higher mean values of serum gamma globulins and serum β2‐microglobulin, and higher prevalence of rheumatoid factor and of antinuclear antibody. Thus, our data demonstrate that BAL permitted the detection of subclinical inflammatory alveolitis in 55% of our patients with primary SS. A long‐term followup is required to determine whether these patients will develop obvious pulmonary involvement.
The Autoimmune Antigen me is Distinct and Related to Undifferentiated Connective Tissue Disease
Authors: Treadwell Edward L. , Boak Andra M. , Kovacs SHIRLEY A. H. , Chen Jintao , Wang Richard J. , Sharp Gordon C. , Agris Paul F. 
Published: February 2007

AbstractUsing prototype Me serum, a new autoantibody‐antigen system has been identified by Ouchterlony immunodiffusion and indirect immunofluorescence. Although immunologically distinct, the Me antigen has physiochemical and biochemical properties similar to those of the Sm antigen. Immunoblot assays indicate that Me sera commonly recognize 4 peptides of molecular weights approximating 100K, 65K, 21K, and 16K. The last of these may be identical to the D peptide recognized by Sm antibodies. The Me antigen may be associated with the RNP‐Sm macromolecular complex. Me‐positive patients have few clinical symptoms, and the most common diagnosis is undifferentiated connective tissue disease.
Cloning of a complementary dna for rabbit proactivator. a metalloproteinase that activates synovial cell collagenase, shares homology with stromelysin and transin, and is coordinately regulated with collagenase
Authors: Fini M. Elizabeth , Karmilowicz Michael J. , Ruby Peggy L. , Beeman Anne M. , Borges Kimberly A. , Brinckerhoff Constance E. 
Published: February 2007

AbstractRabbit proactivator is a neutral metalloproteinase that activates another metalloproteinase, procollagenase, and degrades noncollagenous matrix. We describe the construction of an activator complementary DNA (cDNA) clone, which is 1.9 kb, that selects a 2.1‐kb messenger RNA (mRNA) in Northern blot hybridizations. Nucleic acid sequence studies of the activator cDNA indicate 1) that it encodes protein Mr 53,881, 2) that this protein exhibits ˜80% homology with rat transin, an oncogene‐induced protein with a previously unknown function, and 3) that, in the first 172 residues, it is virtually identical to the rabbit metalloproteinase, stromelysin. Homology between rabbit activator and human skin collagenase is approximately 50%. Activator and collagenase mRNA are coordinately regulated; untreated cultures of rabbit synovial fibroblasts produce low levels of each protein, but addition of phorbol myristate acetate (10–8M) results in an increase in mRNA for both proteins by 2.5–5 hours. Adding all‐trans‐retinoic acid (10–6M) or dexamethasone (10–7M) to phorbol‐stimulated cells coordinately suppresses both activator and collagenase mRNA. Our data suggest the existence of coordinately regulated metalloproteinases that are important in the modulation of connective tissue metabolism.
Imaging of experimental amyloidosis with 131i‐labeled serum amyloid p component
Authors: Caspi Dan , Zalzman Samuel , Baratz Mimi , Teitelbaum Zvi , Yaron Michael , Pras Mordechai , Baltz Marilyn L. , Pepys Mark B. 
Published: February 2007

Abstract131I‐labeled human serum amyloid P component, which was injected into mice with experimentally induced systemic AA amyloidosis and into controls, became specifically localized and was retained in amyloidotic organs. In comparison, it was rapidly and completely eliminated from unaffected tissues and from control animals. Distinctive images of this amyloid‐specific deposition of labeled serum amyloid P component were derived from whole body scanning, in vivo, of amyloidotic mice. These findings suggest that such imaging may have applications for the diagnosis and quantitation of amyloid deposits in humans.