bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physical characteristics, blood hormone concentrations, and plasma lipid concentrations in obese horses with insulin resistance
Authors: Frank Nicholas , Elliott Sarah B. , Brandt Laura E. , Keisler Duane H. 
Published: May 2006

Abstract
Objective—To compare obese horses with insulin resistance (IR) with nonobese horses and determine whether blood resting glucose, insulin, leptin, and lipid concentrations differed between groups and were correlated with combined glucose-insulin test (CGIT) results.
Animals—7 obese adult horses with IR (OB-IR group) and 5 nonobese mares.
Procedures—Physical measurements were taken, and blood samples were collected after horses had acclimated to the hospital for 3 days. Response to insulin was assessed by use of the CGIT, and maintenance of plasma glucose concentrations greater than the preinjection value for ≥ 45 minutes was used to define IR. Area under the curve values for glucose (AUCg) and insulin (AUCi) concentrations were calculated.
Results—Morgan, Paso Fino, Quarter Horse, and Tennessee Walking Horse breeds were represented in the OB-IR group. Mean neck circumference and BCS differed significantly between groups and were positively correlated with AUC values. Resting insulin and leptin concentrations were 6 and 14 times as high, respectively, in the OB-IR group, compared with the nonobese group, and were significantly correlated with AUCg and AUCi. Plasma nonesterified fatty acid, very low-density lipoprotein, and high-density lipoprotein-cholesterol (HDL-C) concentrations were significantly higher (86%, 104%, and 29%, respectively) in OB-IR horses, and HDL-C concentrations were positively correlated with AUC values.
Conclusions and Clinical Relevance—Measurements of neck circumference and resting insulin and leptin concentrations can be used to screen obese horses for IR. Dyslipidemia is associated with IR in obese horses.
Effectiveness of using a mat filled with a peroxygen disinfectant to minimize shoe sole contamination in a veterinary hospital
Authors: Amass Sandra F. , Arighi Mimi , Kinyon Joann M. , Hoffman Lorraine J. , Schneider Jessica L. , Draper D. Kevin 
Published: May 2006

Abstract
Objective—To determine the effectiveness of using a disinfectant mat filled with a peroxygen compound to prevent mechanical transmission of bacteria via contaminated footwear between the food animal ward and common breezeway of a veterinary teaching hospital.
Design—Observational study.
Sample Population—Shoe soles of individuals entering and exiting from the ward.
Procedures—A mat filled with peroxygen disinfectant was placed at the entrance to the food animal ward, and participants wiped each shoe twice on the mat surface (n = 16) or walked on the mat surface but did not wipe their shoes (17) before entering and exiting from the ward. Swab specimens were collected from the shoe soles of participants before and after mat use and submitted for bacterial culture.
Results—For both study days, as participants entered the ward, median number of aerobic bacteria isolated from shoe swab specimens collected prior to use of the disinfectant mat was not significantly different from median number isolated after use of the disinfectant mat. However, as participants exited the ward, median number of aerobic bacteria isolated from shoe swab specimens collected prior to use of the disinfectant mat was significantly higher than median number isolated after use of the disinfectant mat.
Conclusions and Clinical Relevance—Results suggest that placing a mat filled with a peroxygen disinfectant at the exit from the food animal ward of a veterinary teaching hospital may help reduce mechanical transmission of bacteria on the footwear of individuals leaving the ward.
Results of a SNP genome screen in a large Costa Rican pedigree segregating for severe bipolar disorder
Authors: Service Susan , Molina Julio , DeYoung Joseph , Jawaheer Damini , Aldana Ileana , Vu Thuy , Bejarano Julio , Fournier Eduardo , Ramirez Magui , Mathews Carol A. , Davanzo Pablo , Macaya Gabriel , Sandkuijl Lodewijk , Sabatti Chiara , Reus Victor , Freimer Nelson 
Published: May 2006

AbstractWe have ascertained in the Central Valley of Costa Rica a new kindred (CR201) segregating for severe bipolar disorder (BP‐I). The family was identified by tracing genealogical connections among eight persons initially independently ascertained for a genome wide association study of BP‐I. For the genome screen in CR201, we trimmed the family down to 168 persons (82 of whom are genotyped), containing 25 individuals with a best‐estimate diagnosis of BP‐I. A total of 4,690 SNP markers were genotyped. Analysis of the data was hampered by the size and complexity of the pedigree, which prohibited using exact multipoint methods on the entire kindred. Two‐point parametric linkage analysis, using a conservative model of transmission, produced a maximum LOD score of 2.78 on chromosome 6, and a total of 39 loci with LOD scores >1.0. Multipoint parametric and non‐parametric linkage analysis was performed separately on four sections of CR201, and interesting (nominal P‐value from either analysis <0.01), although not statistically significant, regions were highlighted on chromosomes 1, 2, 3, 12, 16, 19, and 22, in at least one section of the pedigree, or when considering all sections together. The difficulties of analyzing genome wide SNP data for complex disorders in large, potentially informative, kindreds are discussed. © 2006 Wiley‐Liss, Inc.
Cardiac events after low osmolar ionic or isosmolar nonionic contrast media utilization in the current era of coronary angioplasty
Authors: Le Feuvre Claude , Batisse Anne , Collet Jean P. , Batisse Jean P , Choussat Rémy , Beygui Farzin , Helft Gérard , Montalescot Gilles , Metzger Jean P 
Published: May 2006

AbstractObjectives:Our study aimed to compare the isosmolar nonionic dimer iodixanol and the low osmolar ionic agent ioxaglate in the current era of percutaneous coronary intervention (PCI), using clopidogrel, enoxaparine, direct stenting, and drug eluting stent.Background:Previous studies have suggested an association between thrombus‐related events and type of contrast media. Methods:Our prospective single‐center study included 498 consecutive patients who were assigned to receive either iodixanol (n = 231) or ioxaglate (n = 267). The primary endpoint was the cumulative rate of in‐hospital major adverse clinical events (MACE). A secondary endpoint was the rate of angiographic or procedural complications. Results:Clinical and angiographic baseline characteristics and procedural data were similar in the 2 groups. A peak anti‐Xa >0.5 IU/ml was obtained in 97% in both groups. Glycoprotein IIb/IIIa inhibitors were used in 42% of patients. Coronary stenting was performed in 91% of patients, with direct stenting in 70%, and drug‐eluting stent in 28% of patients. In‐hospital MACE was more frequent in patients receiving iodixanol compared with those receiving ioxaglate (4.8% vs. 0.3%, P < 0.005). This difference was mainly related to the appearance of a large thrombus during PCI (6% with iodixanol vs. 0.3% with ioxaglate, P < 0.0001). In multivariate analysis, independent predictors of in‐hospital MACE were use of iodixanol (P < 0.01), the higher number of stent used (P < 0.008), bifurcation/ostial lesion (P < 0.01), and balloon dilation before stenting (p < 0.001). Conclusions:In our study reflecting the current era of PCI, thrombus‐related events are more frequent with the isosmolar nonionic dimer iodixanol than with the low osmolar ionic agent ioxaglate. © 2006 Wiley‐Liss, Inc.
Occlusion of a large coronary‐cameral fistula using the Amplatzer vascular plug in a 2‐year old
Authors: Balaguru Duraisamy , Joseph Annette , Kimmelstiel Carey 
Published: May 2006

AbstractA 2‐year‐old girl underwent transcatheter occlusion of a fistula. The fistula originated from the right coronary artery and drained into the right atrium. Three interlocking Gianturco coils were initially placed in the distal portion of the fistula resulting in near‐total occlusion of blood flow. Placing a 12‐mm Amplatzer vascular plug obliterated the proximal, dilated portion of the fistula. Complete occlusion with accurate placement was achieved. Advantages and technical implications of the Amplatzer vascular plug in this clinical setting are discussed. © 2006 Wiley‐Liss., Inc.
A dual wire approach to severe ostial bifurcating renal artery stenosis
Authors: Lorin Jeffrey D. , Hirsh David S. , Attubato Michael J. , Sedlis Steven P. 
Published: May 2006

AbstractPercutaneous intervention with balloon expandable stents has proven to be an effective measure to enhance renal blood flow and control blood pressure in subjects with severe ostial renal artery lesions. A small cohort of these subjects have an ostial bifurcation, which complicates the approach to revascularization. In these cases there is a concern of creating a total side‐branch occlusion during balloon expansion. We report two cases of an ostial lesion at a renal artery bifurcation revascularized by employing a sequential dilatation double guidewire technique. Using a single 7F sheath in each case, both renal artery branches were wired, and each branch was predilated and stented in a sequential fashion. Excellent angiographic results were obtained in both cases. Published 2006 Wiley‐Liss., Inc.
Transcatheter closure of pulmonary arteriovenous malformations with Amplatzer devices
Authors: Beck Avi , Dagan Tamir , Matitiau Avraham , Bruckheimer Elchanan 
Published: May 2006

AbstractObjective:To review the authors' experience with transcatheter closure of pulmonary arteriovenous malformations (PAVMs) using amplatzer duct occluder (ADO) devices and vascular plugs (AGA Medical, Golden Valley, MN) and present a novel technique for delivery sheath placement and device delivery.Background:PAVMs can cause cyanosis, fatigue, polycythemia, and thromboembolic phenomena. Transcatheter closure using coils, detachable balloons, and various devices has replaced surgery as the preferred therapy.Methods:Between January 2001 and December 2004, five patients (2M, 3F) of median age 33 years (14–49) were referred for transcatheter closure of multiple PAVMs. All patients were diagnosed previously with hereditary hemorrhagic telangectasia. The procedures were performed with sedation using a percutaneous transcatheter technique via the femoral vein under fluoroscopic guidance.Results:A total of 14 PAVMs (11 ADO and 3 plugs) were closed in five patients. Three patients required two procedures after developing additional PAVMs. All attempts at PAVM closure were successful. Oxygen saturation increased from 88.4 ± 6.1 to 96.4 ± 0.5 (P < 0.05). No complications, including air or thromboembolism, hemoptysis, or chest pain, occurred. At median follow‐up of 3.4 years (1.4–3.6), all patients are alive and have suffered no embolic phenomena or infection.Conclusion:Amplatzer patent ductus arteriosus occluders and vascular plugs are safe and effective in the closure of PAVMs in the acute setting and at intermediate follow‐up. Placement of a long delivery sheath can be facilitated by the methods outlined. Though promising, further clinical evaluation of these devices is required. Their apparent advantages must be compared to other techniques and devices for transcatheter PAVM closure. © 2006 Wiley‐Liss., Inc.
Feasibility and safety of transbrachial approach for patients with severe carotid artery stenosis undergoing stenting
Authors: Wu Chiung‐Jen , Cheng Cheng‐I , Hung Wei‐Chin , Fang Chih‐Yuan , Yang Cheng‐Hsu , Chen Chien‐Jen , Chen Yen‐Hsun , Hang Chi‐Ling , Hsieh Yuan‐Kai , Chen Shyh‐Ming , Yip Hon‐Kan 
Published: May 2006

AbstractAlthough sporadic successful cases using the transradial approach (TRA) for carotid stenting have been reported, the safety and feasibility of carotid stenting using either TRA or a transbrachial approach (TBA) have not been fully investigated. Recently, we have developed a safe and feasible method of TRA for cerebrovascular angiographic studies. This study investigated whether a TBA approach using a 7‐French (F) Kimny guiding catheter for carotid stenting is safe and feasible for patients with severe carotid stenosis. Thirteen patients were enrolled into this study (age range, 63–78 years). Seven of these 13 patients had severe peripheral vascular disease. A retrograde‐engagement technique, involving looping 6‐F Kimny guiding catheter, was utilized for carotid angiographic study. For carotid stenting, the 6‐F Kimny guiding catheter was replaced with a 7‐F Kimny guiding catheter, and the procedure was performed as the follows. First, an extra‐support wire was inserted into the middle portion of external carotid artery (ECA). Second, a 0.035‐inch Teflon wire was advanced into the common carotid artery. Then, the 6‐F guiding catheter was exchanged for a 7‐F Kimny guiding catheter. Third, if the first and second steps did not provide adequate support for exchanging the guiding catheter, a PercuSurge GuardWire™ was inserted into the ECA, followed by distal balloon inflation for an anchoring support. FilterWire EX™ was used in 9 patients and PercuSurge GuardWire in 4 patients to protect from distal embolization during the procedure. The procedure was successful in all patients. No neurological or vascular bleeding complications were observed and all patients were discharged uneventfully. The TBA for carotid stenting was safe and effective, providing a last resort for patients unsuited to femoral arterial access and surgical intervention. © 2006 Wiley‐Liss., Inc.
Novel double catheter technique in complex percutaneous coronary interventions
Authors: Alhaddad Imad A. 
Published: May 2006

AbstractWe present a novel double catheter technique for successful complex intervention of a very old proximal left circumflex chronic total occlusion (>10 years old). Prior attempts of guide wire passage using bare wire alone, over‐the wire balloon or microcatheter support techniques were unsuccessful. © 2006 Wiley‐Liss, Inc.
Generation of mice harboring a Sox6 conditional null allele
Authors: Dumitriu Bogdan , Dy Peter , Smits Patrick , Lefebvre Véronique 
Published: May 2006

AbstractSox6 belongs to the family of Sry‐related HMG box transcription factors, which determine cell fate and differentiation in various lineages. Sox6 is expressed in several tissues, including cartilage, testis, neuronal, and erythropoietic tissues. Mice lacking Sox6 have revealed critical roles for Sox6 in several of these tissues, but their multiple defects and early lethality has limited studies in specific cell types and in postnatal mice. We show here that we have generated mice harboring a Sox6 conditional null allele (Sox6fl+) by flanking the second coding exon with loxP sites. This allele encodes wildtype Sox6 protein, is expressed normally, and is efficiently converted into a null allele (Sox6fl−) by Cre‐mediated recombination in somatic and germ cells. Sox6fl+/fl+ mice are indistinguishable from wildtype mice, and Sox6fl−/fl− mice from Sox6−/− mice. These Sox6 conditional null mice will thus be valuable for further uncovering the roles of Sox6 in various processes in vivo. genesis 44:219–224, 2006. Published 2006 Wiley‐Liss, Inc.
Mouse ZIP1 and ZIP3 genes together are essential for adaptation to dietary zinc deficiency during pregnancy
Authors: Dufner‐Beattie Jodi , Huang Zhixin L. , Geiser Jim , Xu Wenhao , Andrews Glen K. 
Published: May 2006

AbstractSubfamily II of the solute‐linked carrier 39A superfamily contains three well‐conserved zinc transporters (ZIPs1, 2, 3) whose physiological functions are unknown. We generated mice homozygous for knockout alleles of ZIP1 and both ZIP1 and ZIP 3 (double‐knockout). These mice were apparently normal when dietary zinc was replete, but when dietary zinc was limited during pregnancy embryos from ZIP1 or ZIP3 knockout mice were two to three times more likely to develop abnormally than those in wildtype mice, and 91% (71/78) of embryos developed abnormally in ZIP1, ZIP3 double‐knockout mice. Analysis of the patterns of expression of these genes in mice revealed predominate expression in intestinal stromal cells, nephric‐tubular epithelial cells, pancreatic ductal epithelial cells, and hepatocytes surrounding the central vein. This suggests that these zinc transporters function, at least in part, in the redistribution and/or retention of zinc rather than its acquisition from the diet. In conclusion, mutations in the ZIP1 and ZIP3 zinc transporter genes are silent when dietary intake of zinc is normal, but can dramatically compromise the success of pregnancy when dietary intake of zinc is limiting. genesis 44:239–251, 2006. Published 2006 Wiley‐Liss, Inc.
Adenosine A2A receptor‐mediated cell death of mouse thymocytes involves adenylate cyclase and Bim and is negatively regulated by Nur77
Authors: Kiss Ildikó , Oskolás Henriett , Tóth Réka , Bouillet Philippe , Tóth Katalin , Fülöp András , Scholtz Beáta , Ledent Catherine , Fésüs László , Szondy Zsuzsa 
Published: May 2006

AbstractAdenosine is generated in the microenvironment of emerging thymocytes through normal mechanisms of lymphocyte selection. In a normal thymus, most of the adenosine is catabolized by adenosine deaminase; however, in an environment where up to 95% of the cells undergo programmed cell death, a sufficient amount of adenosine is accumulated to trigger cell surface adenosine receptors. Here we show that accumulated adenosine can induce apoptosis in immature mouse thymocytes, mostly via adenosine A2A receptors. The signaling pathway is coupled to adenylate cyclase activation, induction of the Nur77 transcription factor, Nur77‐dependent genes, such as Fas ligand and TRAIL, and the pro‐apoptotic BH3‐only protein Bim. We analyzed several knockout and transgenic mouse lines and found that adenosine‐induced killing of mouse thymocytes requires Bim, occurs independently of “death receptor” signaling and is inhibited by Bcl‐2 and Nur77. Collectively our data demonstrate that adenosine‐induced cell death involves signaling pathways originally found in negative selection of thymocytes and suggest a determining role of Bim and a regulatory role for Nur77.
Olig gene function in CNS development and disease
Authors: Ligon Keith L. , Fancy Stephen P.J. , Franklin Robin J.M. , Rowitch David H. 
Published: May 2006

AbstractOlig1 and Olig2 encode basic helix‐loop‐helix (bHLH) transcription factors that are expressed in both the developing and mature vertebrate central nervous system. While numerous studies have established critical functions for Olig genes during the formation of motor neurons and oligodendrocytes of the ventral neural tube, their roles at later stages of development and in adulthood have remained relatively obscure. Recent studies, however, reveal that in the fetal dorsal spinal cord and neural progenitor cells of the adult brain, Olig expression continues to mark, and may regulate, the formation of oligodendroglia. Studies of Olig expression in human brain tumors and repair of demyelinating lesions suggest the possibility of additional functions in a variety of neurological diseases. © 2005 Wiley‐Liss, Inc.
Gene‐specific mechanisms of p53 transcriptional control and prospects for cancer therapy
Authors: Resnick‐Silverman Lois , Manfredi James J. 
Published: May 2006

AbstractThe regulation of gene‐specific activation is critical to the tumor suppressor function by p53. p53 is a well‐characterized transcription factor that responds to DNA damage and other genotoxic stresses by the activation of downstream targets that are involved with repair, differentiation, senescence, growth arrest, and apoptosis. Sequence‐specific binding to DNA, conformation, post‐translational modifications, cofactor binding, stability, and subcellular localization all influence the performance of p53. The purpose of this review is to define features that play a key role in gene‐specific activation and to show that these are often incapacitated in cancer cells. Using such knowledge to design selective strategies for the restoration of p53 wild‐type function in cancer cells represents a promising cancer therapy. J. Cell. Biochem. 99: 679–689, 2006. © 2006 Wiley‐Liss, Inc.
A role of the mitotic spindle checkpoint in the cellular response to DNA replication stress
Authors: Duensing Anette , Teng Xiaoyi , Liu Ying , Tseng Michelle , Spardy Nicole , Duensing Stefan 
Published: May 2006

AbstractReplication stress is a frequent and early event during tumorigenesis. Whereas the cellular responses to a persistent block of replication fork progression have been extensively studied, relatively little is known about how cells respond to low‐intensity replication stress. However, transient replication fork perturbations are likely to occur even more frequently in tumor cells than a permanent replication arrest. We report here that transient, low intensity replication stress leads to a rapid activation of the DNA replication checkpoint but to a significantly delayed apoptotic response in a small but significant number of cells. This late apoptotic response was independent of p53 and we found evidence for cell death during mitosis in a proportion of cells. To further explore the role of p53 in the response to replication stress, we analyzed mouse embryonic fibroblasts (MEFs) deficient of p53 in comparison to wild‐type or p63‐ or p73‐deficient MEFs. We detected a significant increase of apoptosis and morphological signs of failed mitosis such as multinucleation in p53‐deficient MEFs following replication stress, but not in wild‐type or p63‐ or p73‐deficient cells. Multinucleated p53‐deficient MEFs frequently retained cyclin B1 expression indicating a persistently activated mitotic spindle checkpoint. Collectively, our results suggest that the cellular response to replication stress involves the mitotic spindle checkpoint in a proportion of cells. These findings imply that the mitotic spindle checkpoint may act in concert with DNA damage and cell‐cycle checkpoints as an early anti‐tumor barrier and provide a possible explanation for its frequent relaxation in human cancer. J. Cell. Biochem. 99: 759–769, 2006. © 2006 Wiley‐Liss, Inc.