bluuebunny/crossref_metadata_2025_split huggingface dataset:
Operation time as a prognostic indicator on postoperative complications following percutaneous nephrolithotomy
Authors: Pakdel Alireza , Mohammadi Abdolreza , Shamshirgaran Amirreza , Zemanati Yar Ehsan , Zareian Baghdadabad Leila , Alaeddini Farshid , Koohi Ortakand Roghayeh , Oliveira Reis Leonardo , Hosseini Seyed Reza , Aghamir Seyed Mohammad Kazem 
Published: February 2025

Objective: We aimed to find an optimal cut-off time for percutaneous nephrolithotomy to prevent complications. Methods: This study enrolled 165 patients aged 18–80 with renal stones ⩾2 cm or ⩾1 cm in lower pole, confirmed via noncontrast computed tomography. Baseline characteristics, stone features, operation time, and anesthesia time were recorded. Logistic regression models were fitted and the ability of the surgery time to predict complications, major complications, and systemic inflammatory response syndrome was evaluated using receiver-operating characteristic curves. Area under the receiver-operating characteristic curve analysis was used as a general indicator of quality. Results: Out of 165 enrolled patients, 157 were analyzed (8 excluded due to follow-up and surgery data issues). The cohort consisted of 115 males (73.2%) and 42 females (26.7%), with a mean (SD) age of 47.4 (12.65) years. Multivariate analysis indicated that longer operation times and lower body mass index correlated with higher complication rates. A cut-off of 65 min for operation time showed 96.8% specificity for predicting complications. Additionally, 47.8% of patients were systemic inflammatory response syndrome positive postprocedure, and operation times were not shown to be predictive of systemic inflammatory response syndrome. Conclusion: Operation time seemed to be a potential risk factor for postpercutaneous nephrolithotomy complications and lowering the operation time could prevent postoperative complications.
Interventions to Modify Psychological Processes in Patients Undergoing Spine Surgery: A Systematic Review
Authors: Skarsgard Matthew , Almojuela Alysa , Gagliardi Martin , Swamy Ganesh , Nicholls Fred , Jacobs W Bradley , Thomas Kenneth C , Soroceanu Alex , Eckenswiller Denise , Soumbasis Elias , Tanguay Rob , Evaniew Nathan 
Published: February 2025

Objectives Among patients undergoing elective spine surgery, psychological processes such as kinesiophobia and pain catastrophizing are associated with postoperative disability and poor quality of life. These represent risk factors which could be modified to improve surgical outcomes. We reviewed perioperative interventions to modify psychological processes and their effects on psychological and surgery-related outcomes. Methods We searched MEDLINE, EMBASE, and Cochrane databases for studies examining any interventions for modifying psychological processes in adult patients undergoing spine surgery. Two reviewers screened studies for eligibility, extracted data in duplicate, and performed risk of bias assessments. Outcomes included pain, disability, quality of life, kinesiophobia, self-efficacy, and pain catastrophizing. Results 368 titles and abstracts were retrieved, of which 27 studies underwent full-text screening. We included 12 studies which reported on 1263 patients. Eight were randomized controlled trials. Interventions included preoperative and postoperative cognitive behavioural therapy, cognitive-behavioural-based physical therapy, a web-based interactive platform, an information booklet, and music therapy. The psychological and surgery-related outcomes of interventions were variable, with some studies reporting significant benefits and others reporting no differences between groups. The greatest potential benefits were found in studies of post-operative cognitive-behavioural-based physical therapy. Risk of bias among studies was high due primarily to lack of blinding and limited standardization of interventions. Conclusions Several interventions to potentially modify psychological processes in patients undergoing spine surgery have been reported. Post-operative cognitive-behavioural-based physical therapy might be associated with improved outcomes, but confidence is limited by inconsistency, risk of bias, and limited long-term follow-up.
No Difference in Chondrocyte Viability Using Manual Versus Custom-Cutting Technique for Matrix-Induced Autologous Chondrocyte Implantation
Authors: Chang Wen-Teh , Atzmon Ran , Vel Monica S. , Lin Kenneth M. , Abrams Geoffrey D. , Sherman Seth L. 
Published: February 2025

Background: Matrix-induced autologous chondrocyte implantation (MACI) was developed for knee cartilage restoration involving seeding autologous chondrocytes onto a collagen membrane. Maintaining chondrocyte viability and achieving proper membrane size and contour are crucial for successful outcomes. Scissor cutting (Sc) has traditionally been used to shape the membrane, but recently, custom cutting (CC) have gained popularity for their enhanced preparation efficiency. Purpose: To determine the difference in chondrocyte viability using the Sc versus CC methods. Study Design: Controlled laboratory study. Methods: Using Sc and CC methods, 15 mm–diameter circular specimens were cut from 5 residual membranes. Membranes were stained, and confocal microscopy was used to visualize live/dead cells. Three zones were defined: the impact zone (the outermost zone at the cut edge), the central zone (the least manipulated zone), and the adjacent zone (the intermediate zone between the central and impact zones). For each circular specimen, the following measurements were recorded: the dimension of the impact zone, cell viability (percentage of live cells among total) for each zone, and the total number of cells within each zone (as cell density ×105/cm2). Results: The width of the impact zone (mean ± standard error) was 355 ± 31 μm and 342 ± 24 μm for CC and Sc, respectively, and the impact zone occupied approximately 10% of the specimen. With both cutting techniques, cell viability was significantly lower in the impact zone (mean ± standard error: CC, 36.42% ± 3.85%; Sc, 40.94% ± 2.85%) compared with the adjacent zone (CC, 77.69% ± 2.97%; Sc, 74.17% ± 2.8%). The cell density from all zones varied from 5.84 ± 0.26 to 6.49 ± 0.34 × 105/cm2, with no significant difference in cell viability in the impact zones between the cutting methods. Conclusion: Both cutting techniques led to a significant reduction in cell viability in the impact zone compared with other zones of the MACI specimen. There was no significant difference in chondrocyte viability or cell density for membranes cut by Sc or CC. Clinical Relevance: This evaluation of the newly introduced CC method on the chondrocyte viability of the MACI membrane will enable surgeons to make a more informed decision regarding cutting techniques.
Evaluation of Pickleball-Related Injuries at a Single Institution From 2017 to 2022
Authors: Herzberg Simone D. , Bowman Eric N. , Hill Kelly L. 
Published: February 2025

Background: Pickleball is currently the fastest-growing sport in the United States, and participation has increased 3-fold throughout the study period. Purpose/Hypothesis: This study aimed to evaluate the epidemiology of pickleball-related injuries, detailing the descriptive data, injury patterns, and health care utilization at a single center over 6 years. It was hypothesized that there would be a substantial increase in pickleball-related injuries during the study period, particularly with increasing age, along with increased orthopaedic visits for pickleball-related injuries. Study Design: Case series; Level of evidence, 4. Methods: We systematically queried the Electronic Health Record System at a single institution from January 1, 2017, to December 31, 2022, to identify patients with injuries associated with pickleball participation. Data were collected on descriptive data, injury characteristics, and health care utilization. Results: Pickleball injuries were confirmed in 487 patients, accounting for a total of 618 injuries. Patient age ranged from 12 to 82 years (mean, 62 years), and 53% of injuries occurred in male patients. The number of injuries increased 6.8-fold from 2017 to 2022. Overall, soft tissue strain or rupture (n = 296; 47.9%) was the most common injury category, followed by arthritis-related pain (n = 62; 10%) and fracture (n = 57; 9.2%). However, age-stratified analysis revealed a shift toward fractures and arthritis-related pain in patients >60 years. Women were 2 times more likely to sustain fractures. Injuries to the rotator cuff were most commonly seen (n = 67, 11%), followed by those to the back (n = 48; 7.8%) and calf (n = 35; 5.6%). Most patients (n = 426; 87%) were initially evaluated by a nonorthopaedic provider, with orthopaedic/sports medicine providers seeing 63% of patients. Conclusion: Our institution observed a 6.8-fold increase in pickleball-related injuries from 2017 to 2022, which far outpaces the reported increase in pickleball participation nationally. Women >60 years were found to be at the highest risk of injury and were more likely to sustain fractures. Most injuries were musculoskeletal related, with soft tissue injury occurring most commonly in patients <60 years and fractures more commonly seen in patients >60 years.
Implementation Outcomes From a Pilot Study of Mindful Awareness in Body-Oriented Therapy (MABT) as a Chronic Pain Treatment Modality in an Integrative Health Clinic
Authors: Hansen Kathryn A. , Abu-Rish Blakeney Erin , Price Cynthia J. 
Published: February 2025

Background As regulation of opioid prescribing evolves, primary care and pain clinics are shifting to provide non-pharmacological and interdisciplinary chronic pain care. An under-utilized but growing area of health care for chronic pain is complementary and integrative health (CIH). However, there is limited availability of CIH approaches within the health care system. Mindful Awareness in Body-Oriented Therapy (MABT) is an evidence-based mind-body therapy, with a manualized protocol, that focuses on developing interoceptive sensibility for improved self-awareness and nervous system regulation. Prior MABT research shows MABT improves self-report and physiological indicators of interoception as well as mental and physical symptoms of distress. Methods This pilot single-group study used a hybrid implementation-effectiveness design and mixed methods to study implementation strategies and outcomes for bringing MABT into an integrative chronic pain clinic. Administrative data, staff surveys, and focus groups were used to understand the implementation process and outcomes (see Additional files 2, 3, and 4). Descriptive statistics were used to analyze survey and administrative data. A content analysis approach was used to analyze qualitative data from focus groups. Results 7 staff surveys were administered over the 24-month study period and showed high acceptability and appropriateness that increased over time. Adoption, feasibility, and sustainability were also high. Clinicians made 70 referrals to MABT, 56 patients scheduled a session, 41 patients completed at least one session, and 71% of these completed the protocol. Focus groups identified MABT as a therapy that filled a gap in services, particularly for patients with a lack of body awareness and high emotion dysregulation. Conclusion Implementation of MABT was highly successful in an integrative health clinic focused on chronic pain treatment. ClinicalTrials.gov registration NCT05289024 Registered March 11, 2022 https://clinicaltrials.gov/study/NCT05289024?term=NCT05289024&rank=1 .
Role of exercise on ncRNAs and exosomal ncRNAs in preventing neurodegenerative diseases: a narrative review
Authors: Liu Shangwu , Zhang Runhong , Hallajzadeh Jamal 
Published: February 2025

Abstract
Engaging in activity has proven to have beneficial effects on different facets of well-being, such as conditions related to the deterioration of the nervous system. Non-coding RNAs (ncRNAs) and exosomal ncRNAs associated with vesicles have been recognized as influencers of gene expression and cell signaling, potentially contributing to the positive impact of physical activity on neurodegenerative conditions. It is hypothesized that exercise-induced changes in ncRNA expression may regulate key processes involved in neuroprotection, including neuroinflammation, oxidative stress, protein aggregation, and synaptic function. Exercise has shown promise in preventing neurodegenerative diseases (NDs), and ncRNAs and exosomal ncRNAs are emerging as potential mediators of these benefits. In review, we explored how ncRNAs and exosomal ncRNAs play a role in enhancing the impacts of activity on neurodegenerative disorders for future treatments. Research studies, both preclinical and clinical, that have documented the use of various exercises and their effects on ncRNAs and exosomal ncRNAs for the treatment of NDs have been compiled and enlisted from the PubMed database, spanning the time period from the year 2000 up to the current time. Studies show that manipulating specific ncRNAs or harnessing exercise-induced changes in ncRNA expression and exosomal cargo could potentially be utilized as therapeutic strategies for preventing or treating NDs. In conclusion, studies suggest that various exercise modalities, including aerobic, resistance, and high-intensity interval training, can modulate the expression of ncRNAs and exosomal ncRNAs in the context of NDs. The altered ncRNA profiles may contribute to the neuroprotective and therapeutic effects observed with exercise interventions. However, more research is needed to fully understand the underlying mechanisms and to further explore the potential of exercise-induced ncRNA signatures as biomarkers and therapeutic targets for neurodegenerative disorders.
Genetic differentiation and historical dynamics of the endemic species Rheum pumilum on the Qinghai-Tibetan Plateau inferred from phylogeography implications
Authors: Liu Shuliang , Gao Jianxin , Xiao Bo , Guo Wenjie , Yu Qiushi , Wang Ailan , Li Weiwei 
Published: February 2025

Abstract

Background

Rheum pumilum, an endemic species on the Qinghai-Tibetan Plateau (QTP), serves as an ideal material for investigating the phylogeography of alpine plants. This study employs chloroplast DNA fragments (trnL-F, trnS-G, and matK) to delve into how Rh. pumilum adapted to the extreme environmental changes on the QTP, during its evolutionary process through phylogenetic geographical analysis, revealing its population differentiation and historical dynamics.

Results
The examination of 39 haplotypes across 26 populations of Rh. pumilum reveals distinct regional distribution, reflecting a phylogeographic pattern resembling “alpine-island”. The total genetic diversity of Rh. pumilum is remarkably high (Ht = 0.910), with the majority of genetic variation primarily occurred among populations (84.5%) with limited gene flow, indicating geographic isolation influenced by diverse habitats of plateau. The geographic isolation model is further supported by various analytical methods, including AMOVA analysis, UPGMA dendrogram, PCoA, Structure analysis, and Mantel test. Micro-refugia for Rh. pumilum during the Quaternary ice ages are supported by haplotype network and genetic diversity analysis. The absence of a typical “star-shape” pattern in the overall haplotype network suggests that Rh. pumilum likely maintains a stable state without experiencing rapid expansion, which has been supported by mismatch distribution analysis. Ecological Niche Modeling (ENM) indicates sensitivity of Rh. pumilum to humidity, temperature and altitude, aligning with a historical distribution resembling a “displacement refugia” model during the Quaternary ice ages. The involvement of Rh. kialense and Rh. sublanceolatum in the origin and gene introgression of Rh. pumilum is suggested, possibly as maternal ancestors of closely related haplotypes. Haplotype divergence of Rh. pumilum approximately 11 million years ago, with notable divergence peaks observed during the late Miocene, as well as the Pliocene, Pleistocene and Holocene.

Conclusion
These findings suggest a correlation between genetic diversity, haplotype lineage divergence and key geological and climatic events, notably the uplift of the QTP, monsoon climate changes, and the climatic oscillations during the Quaternary ice ages. This study might provide valuable insights into the formation mechanisms of plant diversity on the QTP, crucial for biodiversity conservation and sustainable species development in extreme environments.
Clinical and genetic analysis of methylmalonic aciduria in 60 patients from Southern China: a single center retrospective study
Authors: Abid Hiba , Abid Areeba , Sohail Sarah , Jawaid Sara 
Published: February 2025

Abstract
We read with interest the recent publication on methylmalonic aciduria (MMA) and commend the authors for their outstanding contribution. This letter aims to further build upon their work by emphasizing additional aspects to enhance clinical relevance and diagnostic precision. We highlight the variability in serum MMA levels due to dietary intake, renal function, and external factors, advocating for the integration of supplementary diagnostic tools, such as the 1–13 C-propionate oxidation breath test, alongside biomarkers like fibroblast growth factor 21, growth differentiation factor 15, and lipocalin-2, to improve diagnostic accuracy. Additionally, we discuss the limitations of first-tier newborn screening tests due to high false positive rates and recommend second-tier testing using liquid chromatography coupled with tandem mass spectrometry to increase specificity and reduce false positives. Moreover, we address the underestimation of liver dysfunction in MMA patients, noting the need for longitudinal follow-up to capture the progression of liver function abnormalities. This critique is intended to constructively expand the authors’ findings and underscore the importance of a comprehensive diagnostic and management approach to MMA, ultimately improving patient outcomes.
Determination of oral feeding skills in late preterm, early term, and full-term infants using the neonatal oral feeding monitor (NeoSAFE)
Authors: Ecevit Ayse , Erdogan Balkar , Anuk Ince Deniz , Aksu Meltem , Unal Sezin , Turan Özden , Saracoglu Ahmet , Tarcan Aylin 
Published: February 2025

Abstract

Background
Mature oral feeding is a complex function involving numerous muscles and nerves, typically developing between the postmenstrual age of 34–36 weeks in newborn infants. The objective of this study was to analyze the oral feeding skills of healthy late preterm, early term, and full-term infants using a neonatal oral feeding monitor.

Methods
We used the oral feeding parameters reported by NeoSAFE which is a certified medical device, to assess the swallowing and swallow-respiration coordination in newborn infants. Oral feeding parameters were recorded over a 2-minutes long bottle-feeding session. The total swallow count, swallow time, maximum rhythmic swallows, resting interval duration, time between rhythmic swallows and inspiration after swallow count were recorded by NeoSAFE. We planned to examine the relationship of oral feeding parameters according to the gestational age. We also investigated whether the coordination of swallowing and respiration changes with respect to gestational age in newborn infants.

Results
A total of 88 infants were included; 34 late preterm, 34 early term, and 20 full term. The gestational age was found to have significant negative correlation with the average time between rhythmic swallows and positive correlation with the swallow time. Feeding volume was found to have a negative correlation with the resting interval duration and average time between rhythmic swallows. It was also found that the feeding volume has a positive correlation with total swallow count, swallow time, maximum rhythmic swallow and inspirium after swallow count.

Conclusion
Although the oral feeding skills of infants at 34 weeks gestation are still developing, this study identified differences in oral feeding skills among late preterm, early term, and full-term infants when assessed using a neonatal swallow and respiration detection system. However, conducting larger cohort studies using NeoSAFE would be beneficial for guiding oral feeding approaches in infants.

Trial Registration
Not applicable.
Physiology and pathophysiology of mucus and mucolytic use in critically ill patients
Authors: Roe Thomas , Talbot Thomas , Terrington Isis , Johal Jayant , Kemp Ivan , Saeed Kordo , Webb Elizabeth , Cusack Rebecca , Grocott Michael P. W. , Dushianthan Ahilanandan 
Published: February 2025

Abstract
Airway mucus is a highly specialised secretory fluid which functions as a physical and immunological barrier to pathogens whilst lubricating the airways and humifying atmospheric air. Dysfunction is common during critical illness and is characterised by changes in production rate, chemical composition, physical properties, and inflammatory phenotype. Mucociliary clearance, which is determined in part by mucus characteristics and in part by ciliary function, is also dysfunctional in critical illness via disease related and iatrogenic mechanisms. The consequences of mucus dysfunction are potentially devastating, contributing to prolonged ventilator dependency, increased risk of secondary pneumonia, and worsened lung injury. Mucolytic therapies are designed to decrease viscosity, improve expectoration/suctioning, and thereby promote mucus removal. Mucolytics, including hypertonic saline, dornase alfa/rhDNase, nebulised heparin, carbocisteine/N-Acetyl cysteine, are commonly used in critically ill patients. This review summarises the physiology and pathophysiology of mucus and the existing evidence for the use of mucolytics in critically ill patients and speculates on journey to individualised mucolytic therapy.
Understanding Cognition, Oxytocin, and Pain in Elders (UCOPE): protocol for a double-blinded cross-over trial in chronic knee osteoarthritis pain
Authors: Cruz-Almeida Yenisel , Montesino-Goicolea Soamy , Valdes-Hernandez Pedro , Huo Zhiguang , Staud Roland , Ebner Natalie C. 
Published: February 2025

Abstract

Background
Osteoarthritis (OA) is the leading cause of disability among older adults with the knee being the most affected joint. Specifically, there is an urgent need to develop better analgesics for individuals with OA-related pain, since currently used analgesics frequently fail to provide adequate relief or must be discontinued owing to adverse effects. A promising treatment is the neuropeptide oxytocin (OT), which has been shown to play a role in endogenous analgesia with human and animal studies demonstrating anti-nociceptive effects. The primary aims of the study are to examine preliminary analgesic effects of a chronic OT intervention in community-dwelling older individuals with chronic knee osteoarthritis pain.

Methods
In this article, we describe the rationale and design of the Understanding Cognition, Oxytocin, and Pain in Elders (UCOPE) study, a double-blinded intervention in which 80 participants over 45 years of age with knee osteoarthritis pain will be recruited to participate in a cross-over trial of 4 weeks of intranasal oxytocin or placebo administration. Primary study outcomes include preliminary changes in pain intensity and interference as well as multi-modal assessment batteries including circulating biomarkers and neuroimaging measures. Self-reported and quantitative outcomes will be assessed at baseline, post-intervention periods, and up to a 6-month follow-up period.

Discussion
This study will establish preliminary effectiveness of a novel intervention in middle to older aged adults with knee osteoarthritis pain. Achievement of these aims will provide a rich platform for future intervention research targeting improvements in pain and disability among geriatric populations and will serve as a foundation for a fully powered trial to examine treatment efficacy and potential mechanisms of the proposed intervention.

Trial registration
ClinicalTrials.gov NCT03878589. Registered on March 18th, 2019.