bluuebunny/crossref_metadata_2025_split huggingface dataset:
A randomized controlled trial demonstrating sustained benefit of autologous matrix-induced chondrogenesis (AMIC®) over microfracture: 10-year follow-up
Authors: Volz Martin , Schaumburger Jens , Gellißen Jörg , Grifka Joachim , Anders Sven 
Published: April 2024

Abstract
Purpose
Autologous matrix-induced chondrogenesis (AMIC®) and microfracture are established treatments for focal chondral defects in the knee, but there are little clinical data concerning these procedures over the long term. This study evaluates the outcomes of AMIC® compared to microfracture over 10-year follow-up.

Methods
Forty-seven patients were randomized and treated either with MFx (n = 13), sutured AMIC® (n = 17) or glued AMIC® (n = 17) in a prospective, randomized, controlled multicentre trial. The Modified Cincinnati Knee Score, a visual analogue scale for pain and MOCART score were used to assess outcomes over 10 years post-operatively.

Results
All treatment arms improved in the first 2 years, but a progressive and significant deterioration in scores was observed in the MFx group, while both AMIC® groups remained stable. MOCART scores were comparable between groups.

Conclusion
The AMIC® procedure results in improved patient outcomes in comparison with microfracture up to 10 years following surgery for the repair of focal chondral defects in the knee.
ClinicalTrials.gov Identifier: NCT02993510
A Computational Pipeline to Investigate Longitudinal Blood Flow Changes in the Circle of Willis of Patients with Stable and Growing Aneurysms
Authors: Coccarelli Alberto , Van Loon Raoul , Chien Aichi 
Published: April 2024

AbstractChanges in cerebral blood flow are often associated with the initiation and development of different life-threatening medical conditions including aneurysm rupture and ischemic stroke. Nevertheless, it is not fully clear how haemodynamic changes in time across the Circle of Willis (CoW) are related with intracranial aneurysm (IA) growth. In this work, we introduced a novel reduced-order modelling strategy for the systematic quantification of longitudinal blood flow changes across the whole CoW in patients with stable and unstable/growing aneurysm. Magnetic Resonance Angiography (MRA) images were converted into one-dimensional (1-D) vessel networks through a semi-automated procedure, with a level of geometric reconstruction accuracy controlled by user-dependent parameters. The proposed pipeline was used to systematically analyse longitudinal haemodynamic changes in seven different clinical cases. Our preliminary simulation results indicate that growing aneurysms are not necessarily associated with significant changes in mean flow over time. A concise sensitivity analysis also shed light on which modelling aspects need to be further characterized to have reliable patient-specific predictions. This study poses the basis for investigating how time-dependent changes in the vasculature affect the haemodynamics across the whole CoW in patients with stable and growing aneurysms.
Ambient polycyclic aromatic hydrocarbon exposure and breast cancer risk in a population-based Canadian case–control study
Authors: Hinton Patrick , Villeneuve Paul J. , Galarneau Elisabeth , Larsen Kristian , Wen Deyong , Meng Jun , Savic-Jovcic Verica , Zhang Junhua , King Will D. 
Published: April 2024

Abstract
Purpose
Polycyclic aromatic hydrocarbons (PAHs) represent a class of ubiquitous pollutants recognized as established human carcinogens and endocrine-disrupting chemicals. PAHs have seldom been modeled at the population-level in epidemiological studies. Fluoranthene is a prevalent PAH in urban settings and correlates with the occurrence of other PAHs. The purpose of this study was to evaluate associations between long-term residential exposure to ambient PAHs and breast cancer risk, both pre- and post-menopausal, in Canada.

Methods
Using the National Enhanced Cancer Surveillance System (NECSS), a national-scale Canadian population-based case–control study, annual fluoranthene exposures were estimated using the GEM-MACH-PAH chemical transport model on the basis of geocoded residential histories throughout a 20-year exposure window. Odds ratios (ORs) and 95% confidence intervals (CIs) controlling for potential confounders were estimated using logistic regression. Separate analyses were conducted for Ontario and national samples given a finer-resolution exposure surface and additional risk factor information available for Ontario.

Results
Positive associations were observed between fluoranthene exposure and premenopausal breast cancer, with inconsistent findings for postmenopausal breast cancer. For premenopausal breast cancer, adjusted ORs of 2.48 (95% CI: 1.29, 4.77) and 1.59 (95% CI: 1.11, 2.29) were observed when comparing the second highest category of exposure to the lowest, among the Ontario and national samples, respectively. For postmenopausal breast cancer, adjusted ORs were 1.10 (95% CI: 0.67, 1.80) and 1.33 (95% CI: 1.02, 1.73). Associations for the highest level of exposure, across both samples and menopausal strata, were non-significant.

Conclusion
This study provides support for the hypothesis that ambient PAH exposures increase the risk of premenopausal breast cancer.
Multi-modal Battle Royale optimizer
Authors: Çiçek K. Dilşad , Akan Taymaz , Bayat Oğuz 
Published: April 2024

AbstractMultimodal optimization poses a challenging problem in the field of optimization as it entails the discovery of multiple local and global optima, unlike unimodal optimization, which seeks a single global solution. In recent years, the significance of addressing multimodal optimization challenges has grown due to the real-world complexity of many problems. While numerous optimization methods are available for unimodal problems, multimodal optimization techniques have garnered increased attention. However, these approaches often grapple with a common issue: the determination of the niching parameter, necessitating prior knowledge of the problem space. This paper introduces a novel multimodal optimization approach that circumvents the need for prior problem space knowledge and avoids the challenge of predefining the niching parameter. Building upon the Battle Royal Optimization (BRO) algorithm, this extended version formulates a multimodal solution by utilizing Coulomb's law to identify suitable neighbors. The incorporation of Coulomb's law serves the dual purpose of identifying potential local and global optima based on fitness values and establishing optimal distances from solution candidates. A comparison study was done between the MBRO and seven well-known multimodal optimization algorithms using 14 benchmark problems from the CEC 2013 and CEC 2015 competitions to see how well it worked. The experimental results underscore MBRO's proficiency in successfully identifying most, if not all, local and global optima, positioning it as a superior solution when compared to its competitors.
Submission to Journal of Polymers and the Environment Evaluation of Thermal Properties and Crystallinity in PHB-Based Systems – A DoE Approach
Authors: Majerczak Katarzyna , Liggat John J. 
Published: April 2024

AbstractComplex formulations based on poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate-co-valerate) (PHBV) were studied to statistically assess the effect of formulation (i.e., hydroxyvalerate (HV) content, plasticiser chemistry and content, filler type and content) on their thermal properties and degree of crystallinity (Xc). In binary systems, thermal properties were mainly influenced by filler type rather than its content, while for plasticised systems the changes were dependent on both increasing plasticiser content and PHB-plasticiser compatibility. Variations in HV content affected the ability of the polymer chain to fold, leading to significant changes in both thermal properties and Xc. In ternary systems, presence of multiple additives and consequent changes in intermolecular interactions lead to multifaceted behaviours that were not easily predicted by results from binary systems alone. For example, melting temperature did not show dependence on filler presence in PHBV systems despite introducing variations in pure PHB systems. In general, thermal properties and Xc are affected by all parameters studied, with changes in system free volume (i.e. changes in HV content and plasticisation) playing the most significant role. These results expand the understanding of factors controlling crystallisation in complex polymer systems and can be used to control matrix properties in new generations of packaging materials.
Bacteria from the skin of amphibians promote growth of Arabidopsis thaliana and Solanum lycopersicum by modifying hormone-related transcriptome response
Authors: Romero-Contreras Yordan J. , González-Serrano Francisco , Bello-López Elena , Formey Damien , Aragón Wendy , Cevallos Miguel Ángel , Rebollar Eria A. , Serrano Mario 
Published: April 2024

AbstractPlants and microorganisms establish beneficial associations that can improve their development and growth. Recently, it has been demonstrated that bacteria isolated from the skin of amphibians can contribute to plant growth and defense. However, the molecular mechanisms involved in the beneficial effect for the host are still unclear. In this work, we explored whether bacteria isolated from three tropical frogs species can contribute to plant growth. After a wide screening, we identified three bacterial strains with high biostimulant potential, capable of modifying the root structure of Arabidopsis thaliana plants. In addition, applying individual bacterial cultures to Solanum lycopersicum plants induced an increase in their growth. To understand the effect that these microorganisms have over the host plant, we analysed the transcriptomic profile of A. thaliana during the interaction with the C32I bacterium, demonstrating that the presence of the bacteria elicits a transcriptional response associated to plant hormone biosynthesis. Our results show that amphibian skin bacteria can function as biostimulants to improve agricultural crops growth and development by modifying the plant transcriptomic responses.
On the performance of Sargassum-derived calcium alginate ion exchange resins for Pb2+ adsorption: batch and packed bed applications
Authors: Mohammed Akeem , Mohammed Chantal , Mautner Andreas , Kistow Matika , Chaitram Pooran , Bismarck Alexander , Ward Keeran 
Published: April 2024

AbstractDriven by climate change and human activity, Sargassum blooming rates have intensified, producing copious amount of the invasive, pelagic seaweed across the Caribbean and Latin America. Battery recycling and lead-smelter wastes have heavily polluted the environment and resulted in acute lead poisoning in children through widespread heavy metal contamination particular in East Trinidad. Our study details a comprehensive investigation into the use of Sargassum (S. natans), as a potential resource-circular feedstock for the synthesis of calcium alginate beads utilized in heavy metal adsorption, both in batch and column experiments. Here, ionic cross-linking of extracted sodium alginate with calcium chloride was utilized to create functional ion-exchange beads. Given the low quality of alginates extracted from Sargassum which produce poor morphological beads, composite beads in conjunction with graphene oxide and acrylamide were used to improve fabrication. Stand-alone calcium alginate beads exhibited superior Pb2+ adsorption, with a capacity of 213 mg g−1 at 20 °C and pH 3.5, surpassing composite and commercial resins. Additives like acrylamide and graphene oxide in composite alginate resins led to a 21–40% decrease in Pb2+ adsorption due to reduced active sites. Column operations confirmed Alginate systems’ practicality, with 20–24% longer operating times, 15 times lower adsorbent mass on scale-up and 206% smaller column diameters compared to commercial counterparts. Ultimately, this study advocates for Sargassum-based Alginate ion-exchange beads as a bio-based alternative in Trinidad and developing nations for dealing with heavy metal ion waste, offering superior heavy metal adsorption performance and supporting resource circularity.
Graphical Abstract
Vitreous humor in the forensic toxicology of quetiapine and its metabolites
Authors: Moschovakou Danai , Ntoupa Stamatina-Panagoula , Dona Artemisia , Athanaselis Sotirios , Spiliopoulou Chara , Nikolaou Panagiota , Papoutsis Ioannis 
Published: April 2024

Abstract
Purpose
Τhe aim of the present study was to investigate the use of vitreous humor as an alternative biological material in forensic toxicology for the determination of quetiapine, 7-hydroxy-quetiapine, and nor-quetiapine. The distribution of these substances in vitreous humor was studied by determining and correlating their concentrations in vitreous humor with the respective concentrations in blood.

Methods
During this study, a method for the determination of these substances was developed, validated and applied to postmortem samples obtained from 16 relative forensic cases. The sample preparation procedure included the isolation of the analytes from vitreous humor and blood samples using solid-phase extraction, with Bond Elut LRC C18 columns followed by derivatization with BSTFA with 1% TMCS prior to GC/MS analysis.

Results
The developed method is characterized by a dynamic range of 10.0–1000.0 ng/mL (R2 ≥ 0.991) for the three substances, with a limit of detection and quantification of 3.0 and 10.0 ng/mL, respectively. Accuracy and precision were below 8.09% and 8.99%, respectively, for both biological materials, while absolute recovery for the three substances was greater than 81%. According to the results, quetiapine, 7-hydroxy-quetiapine, and nor-quetiapine are easily distributed in vitreous humor.

Conclusion
The results of the study indicate the usefulness of vitreous humor in toxicological analysis for the determination of these substances, especially when the traditional biological materials are not available. The levels of quetiapine and its metabolites in vitreous humor as well as the vitreous humor to blood concentration ratios can provide important information for a more thorough toxicological investigation of forensic cases.
The interplay between I and We intentionality
Authors: Rizzo Antonio , Boucher Cynthia , Carnesecchi Marco 
Published: April 2024

AbstractWe and I intentionality appear to be two distinct forms of human intentionality, as one cannot be explained in terms of the other. We-intentionality is part of the psychological infrastructure at the basis of human cooperative behavior, while I-intentionality is potentially more related to competitive relationships with conspecifics. Our work tries to empirically address the relationship between these two forms of human intentionality as exhibited during the early stages of human development. The experimental setting consisted of four different games, two competitive and two cooperative. We focused our experiment on three age groups and schooling: Early Elementary School Children (mean = 5 years 6 months; σ = 4.2 months), Late Elementary School Children (mean = 9 years 4 months; σ = 7.5 months), and Adult University students (mean = 21 years; σ = 11 months). The key aspect of the investigation was that only one participant was informed of the game, rules, and reward. The second participant came to the set uninformed. It was the first participant's decision whether and how to engage the latter in the game. We were especially interested in the communicative behaviors: when and how the informed participant would share his or her information. We observed that the Adult University Informed participants shared their information with the Adult University Uninformed participants, while this almost never happened in Early Elementary School Children. Late Elementary School Children presented a split halfway between keeping and sharing the information. The results seem to support the hypothesis of a developmental relationship between the two forms of intentions. They also suggest that the two forms of intentionality are complementary. Each plays a specific role in human relationships with social and physical environments: the We-intentionality would establish the common ground within which the I-intentionality would manifest itself.
Microwave-assisted sumac based biocatalyst synthesis for effective hydrogen production
Authors: Canpolat Gurbet 
Published: April 2024

AbstractHydrogen (H2), a renewable energy source with a high energy density and a reputation for being environmentally benign, is being lauded for its potential in various future applications. In the present context, the catalytic methanolysis of sodium borohydride (NaBH4) is of considerable importance due to its provision of a pathway for the efficient production of hydrogen gas (H2). The main aim of this research attempt was to assess the viability of utilizing refuse defatted sumac seeds as an unusual precursor in microwave-assisted K2CO3 activation to produce a biocatalyst.The primary objective that motivated the synthesis of the biocatalyst was to facilitate the generation of hydrogen via the catalytic methanolysis of NaBH4. With the aim of developing a biocatalyst characterized by enhanced catalytic performance, we conducted an exhaustive investigation of a wide range of experimental parameters. The activation agent-to-sample ratio (IR), impregnation time, microwave power, and irradiation time were among these parameters.Significantly enhanced in catalytic activity, the biocatalyst produced under particular conditions achieved a peak hydrogen production efficiency of 10,941 mL min− 1 g.cat− 1. In particular, it was determined that the ideal conditions were as follows: 0.5 IR, 24 h of impregnation, 500 W of microwave power, and 10 min of irradiation. This novel strategy not only demonstrates the impressive potential of eco-friendly biocatalysts, but also positions them as a viable alternative material for the sustainable production of hydrogen via NaBH4 methanolysis.Three significant parameters contribute to the value and renewability of this study. The first is that waste is used as the primary material; the second is that the activator is less hazardous than other activators; and the third is that microwave activation is a green chemistry technique.
Graphical Abstract
Characterization and evaluation of photolabile (µ-peroxo)(µ-hydroxo)bis[bis(bipyridyl)cobalt caged oxygen compounds to facilitate time-resolved crystallographic studies of cytochrome c oxidase
Authors: Sandelin Emil , Johannesson Jonatan , Wendt Ola , Brändén Gisela , Neutze Richard , Wallentin Carl-Johan 
Published: April 2024

AbstractPhotolabile (µ-peroxo)(µ-hydroxo)bis[bis(bipyridyl)-cobalt-based caged oxygen compounds have been synthesized and characterized by optical absorbance spectroscopy, X-ray crystallography. and the quantum yield and redox stability were investigated. Furthermore, conditions were established where redox incompatibilities encountered between caged oxygen compounds and oxygen-dependant cytochrome c oxidase (CcO) could be circumvented. Herein, we demonstrate that millimolar concentrations of molecular oxygen can be released from a caged oxygen compound with spatio-temporal control upon laser excitation, triggering enzymatic turnover in cytochrome c oxidase. Spectroscopic evidence confirms the attainment of a homogeneous reaction initiation at concentrations and conditions relevant for further crystallography studies. This was demonstrated by the oxidizing microcrystals of reduced CcO by liberation of millimolar concentrations of molecular oxygen from a caged oxygen compound. We believe this will expand the scope of available techniques for the detailed investigation of oxygen-dependant enzymes with its native substrate and facilitate further time-resolved X-ray based studies such as wide/small angle X-ray scattering and serial femtosecond crystallography.
Graphical abstract
A coordinated switch in sucrose and callose metabolism enables enhanced symplastic unloading in potato tubers
Authors: van den Herik Bas , Bergonzi Sara , Li Yingji , Bachem Christian W. , ten Tusscher Kirsten H. 
Published: April 2024

Abstract
One of the early changes upon tuber induction is the switch from apoplastic to symplastic unloading. Whether and how this change in unloading mode contributes to sink strength has remained unclear. In addition, developing tubers also change from energy to storage-based sucrose metabolism. Here, we investigated the coordination between changes in unloading mode and sucrose metabolism and their relative role in tuber sink strength by looking into callose and sucrose metabolism gene expression combined with a model of apoplastic and symplastic unloading. Gene expression analysis suggests that callose deposition in tubers is decreased by lower callose synthase expression. Furthermore, changes in callose and sucrose metabolism are strongly correlated, indicating a well-coordinated developmental switch. Modelling indicates that symplastic unloading is not the most efficient unloading mode per se. Instead, it is the concurrent metabolic switch that provides the physiological conditions necessary to potentiate symplastic transport and thereby enhance tuber sink strength .
First report of Leucinodes africensis and Leucinodes laisalis on Solanum aethiopicum and Solanum melongena in farmer's fields in southern Ghana
Authors: Fening Ken Okwae , Okyere Stanley Osafo , Forchibe Ethelyn Echep , Layodé Babatoundé Ferdinand Rodolphe , Richmond Tegbe Enyonam , Agboyi Lakpo Koku B. A. , Afreh-Nuamah Kwame , Wamonje Francis Onono 
Published: April 2024

AbstractThe eggplant fruit and shoot borer (EFSB) is a devastating pest of eggplants (Solanum aethiopicum L. and Solanum melongena L.) in Ghana, causing significant economic losses. Although initially thought to be the Leucinodes orbonalis Guenee species found in Asia, recent European and Mediterranean Plant Protection Organization reports suggest its absence in Africa. However, eight Leucinodes species have been recently described in Africa, including two new species, Leucinodes africensis sp. n. and Leucinodes laisalis Walker, which were intercepted in eggplant fruits exported from Ghana to the United Kingdom. Despite the reported absence of L. orbonalis in Africa, it remains on the pest list of Ghana as a species known to attack eggplants. To accurately determine the identity of the EFSB complex occurring on eggplant in Southern Ghana, molecular and morphological taxonomic tools were employed, and adult male populations were monitored in on-farm conditions. Our results revealed the presence of two EFSB species, L. africensis and L. laisalis, in the shoot and fruits of eggplants, with L. africensis being the dominant species and widely distributed in Southern Ghana. Notably, L. africensis males were attracted to the pheromone lure of L. orbonalis despite the two species being biologically distinct. This study provides crucial information on correctly identifying the EFSB species attacking eggplants in Southern Ghana and has significant implications for developing management interventions against these pests and their effects on international eggplant trade.