bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent patterns in gastric cancer: A global overview
Authors: Bertuccio Paola , Chatenoud Liliane , Levi Fabio , Praud Delphine , Ferlay Jacques , Negri Eva , Malvezzi Matteo , La Vecchia Carlo 
Published: January 2009

AbstractUntil the mid‐1990s, gastric cancer has been the first cause of cancer death worldwide, although rates had been declining for several decades and gastric cancer has become a relatively rare cancer in North America and in most Northern and Western Europe, but not in Eastern Europe, Russia and selected areas of Central and South America or East Asia. We analyzed gastric cancer mortality in Europe and other areas of the world from 1980 to 2005 using joinpoint regression analysis, and provided updated site‐specific incidence rates from 51 selected registries. Over the last decade, the annual percent change (APC) in mortality rate was around −3, −4% for the major European countries. The APC were similar for the Republic of Korea (APC = −4.3%), Australia (−3.7%), the USA (−3.6%), Japan (−3.5%), Ukraine (−3%) and the Russian Federation (−2.8%). In Latin America, the decline was less marked, but constant with APC around −1.6% in Chile and Brazil, −2.3% in Argentina and Mexico and −2.6% in Colombia. Cancers in the fundus and pylorus are more common in high incidence and mortality areas and have been declining more than cardia gastric cancer. Steady downward trends persist in gastric cancer mortality worldwide even in middle aged population, and hence further appreciable declines are likely in the near future. © 2009 UICC
Activated leukocyte cell adhesion molecule: A novel biomarker for breast cancer
Authors: Kulasingam Vathany , Zheng Yingye , Soosaipillai Antoninus , Leon Antonette E. , Gion Massimo , Diamandis Eleftherios P. 
Published: January 2009

AbstractActivated leukocyte cell adhesion molecule (ALCAM) has been implicated in tumorigenesis. Our goal was to examine the levels of ALCAM, in addition to the classical breast cancer tumor markers carbohydrate antigen 15‐3 (CA15‐3) and carcinoembryonic antigen (CEA), in serum by quantitative enzyme‐linked immunosorbent assay for diagnosis in breast cancer patients. The 3 proteins were measured in serum of 100 healthy women, 50 healthy men and 150 breast carcinoma patients. The diagnostic sensitivity and specificity of the tests were calculated and the association of serum marker concentrations with various clinicopathologic variables was examined using nonparametric Kruskal‐Wallis tests. Receiver operating characteristic (ROC) curves were used to evaluate the diagnostic performance of the biomarkers. ALCAM, with area under the curve (AUC) of 0.78 [95% CI: 0.73, 0.84] outperformed CA15‐3 (AUC = 0.70 [95% CI: 0.64, 0.76]) and CEA (AUC= 0.63 [95% CI: 0.56, 0.70]). The incremental values of AUC for ALCAM over that for CA15‐3 were statistically significant (Delong test, p < 0.05). Combining CA15‐3 and ALCAM yielded a ROC curve with an AUC of 0.81 (95% CI [0.75, 0.87]). Serum ALCAM appears to be a new biomarker for breast cancer and may have value for disease diagnosis. © 2009 UICC
Retracted: Inhibition of non‐neuronal α7‐nicotinic receptor reduces tumorigenicity in A549 NSCLC xenografts
Authors: Paleari Laura , Sessa Fausto , Catassi Alessia , Servent Denis , Mourier Gilles , Doria‐Miglietta Guido , Ognio Emanuela , Cilli Michele , Dominioni Lorenzo , Paolucci Massimo , Calcaterra Andrea , Cesario Alfredo , Margaritora Stefano , Granone Pierluigi , Russo Patrizia 
Published: January 2009

AbstractRetraction: The following article from International Journal of Cancer, "Inhibition of non‐neuronal α7‐nicotinic receptor reduces tumorigenicity in A549 NSCLC xenografts", by Laura Paleari, Fausto Sessa, Alessia Catassi, Denis Servent, Gilles Mourier, Guido Doria‐Miglietta, Emanuela Ognio, Michele Cilli, Lorenzo Dominioni, Massimo Paolucci, Andrea Calcaterra, Alfredo Cesario, Stefano Margaritora, Pierluigi Granone and Patrizia Russo, published online in Wiley InterScience (http://www3.interscience.wiley.com) on 22 January 2009, Volume 125, Issue 1, pages 199‐211, has been retracted by agreement between the authors, the journal Editor in Chief, Peter Lichter, and Wiley Periodicals, Inc. The retraction has been agreed due to unverified data.
Mitochondrial potassium channels
Authors: Szewczyk Adam , Jarmuszkiewicz Wieslawa , Kunz Wolfram S. 
Published: January 2009

AbstractMitochondrial potassium channels are believed to contribute to cytoprotection of injured cardiac and neuronal tissues. The following potassium channels have been described in the inner mitochondrial membrane: the ATP‐regulated potassium channel, the large conductance Ca2+‐activated potassium channel, the voltage‐gated Kv1.3 potassium channel, and the twin‐pore domain TASK‐3 potassium channel. The putative functional roles of these channels include changes in mitochondrial matrix volume, mitochondrial respiration, and membrane potential. In addition, the activity of these channels modulates the generation of reactive oxygen species by mitochondria. In this article, we discuss recent observations on three fundamental issues concerning mitochondrial potassium channels: (i) their molecular identity, (ii) their interaction with potassium channel openers and inhibitors, and (iii) their functional properties. © 2009 IUBMB IUBMB Life, 61(2): 134–143, 2009
Origin and evolution of the genetic code: The universal enigma
Authors: Koonin Eugene V. , Novozhilov Artem S. 
Published: January 2009

AbstractThe expansion of the standard code according to the coevolution theory. Phase 1 amino acids are orange, and phase 2 amino acids are green. The numbers show the order of amino acid appearance in the code according to (99). The arrows define 13 precursor‐product pairs of amino acids, their color defines the biosynthetic families of Glu (blue), Asp (dark‐green), Phe (magenta), Ser (red), and Val (light‐green). See Origin and Evolution of the Genetic Code: The Universal Enigma by Koonin and Novozhilov, pp. 99‐111.
Improving the affinity of fibroblasts for bacterial cellulose using carbohydrate‐binding modules fused to RGD
Authors: Andrade F. K. , Moreira S. M G. , Domingues L. , Gama F. M. P. 
Published: January 2009

AbstractThe attachment of cells to biomedical materials can be improved by using adhesion sequences, such as Arg‐Gly‐Asp (RGD), found in several extracellular matrix proteins. In this work, bifunctional recombinant proteins, with a Cellulose‐Binding Module (CBM), from the cellulosome of Clostridium thermocellum and cell binding sequences—RGD, GRGDY—were cloned and expressed in E.coli. These RGD‐containing cellulose binding proteins were purified and used to coat bacterial cellulose fibres. Its effect on the cell adhesion/biocompatibility properties was tested using a mouse embryo fibroblasts culture. Bacterial cellulose (BC) secreted by Gluconacetobacter xylinus (=Acetobacter xylinum) is a material with unique properties and promising biomedical applications. CBMs adsorbs specifically and tightly on cellulose. Thus, they are a useful tool to address the fused RGD sequence (or other bioactive peptides) to the cellulose surface, in a specific and simple way. Indeed, fibroblasts exhibit improved ability to interact with bacterial cellulose sheets coated with RGD‐CBM proteins, as compared with cellulose treated with the CBM, that is, without the adhesion peptide. The effect of the several fusion proteins produced was analyzed. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
In vitro elution of moxifloxacin from cancellous bone allografts
Authors: Kanellakopoulou Kyriaki , Sahinides Theophilos , Tsaganos Thomas , Galanakis Nearchos , Giamarellou Helen , Giamarellos‐Bourboulis Evangelos J. 
Published: January 2009

AbstractThe characteristics of cancellous bone allografts as carriers of moxifloxacin are described. Particles of cancellous bone were compressed into a wire‐mesh cylinder and impregnated into a solution of moxifloxacin for different time periods. Five replicas were impregnated for 1 h; another five for 24 h; and another five for 48 h. Impregnated allografts were then transferred into vials containing 5 ml of Mueller‐Hinton broth and incubated at 37°C. Broth was replaced daily. Concentrations of moxifloxacin in broth were determined after analysis by an high performance liquid chromatography system. Moxifloxacin was eluted at very high concentrations within the first days. Concentrations remained above 100 μg/ml until day 8 and above 40 μg/ml until day 20. It is concluded that cancellous bone allografts may allow the adequate in vitro elution of moxifloxacin. The latter results support their application in experimental models of osteomyelitis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Experiment, thermal simulation, and characterizations on transmission laser coating of hydroxyapatite on metal implant
Authors: Cheng Gary J. , Ye Chang 
Published: January 2009

AbstractCoating of bioceramic material, Hydroxyapatite (HAp), on metal implant has attracted many attentions in biomedical industry recently because its combination of good mechanical property and biocompatibility. However, most of current HAp coatings lack coating/substrate interfacial strength, and/or biocompatibility. The cell‐tissue attachment is affected by the degraded biocompatibility due to decomposition of HAp during high temperature processing. In this article, an innovative method, transmission laser coating (TLC), is investigated to coat HAp on Ti substrate with low temperature processing. This process enhances the HAp/Metal interfacial property of current coatings, while maintaining good biocompatibility. Experiments are conducted using a continuous neodymium‐doped yttrium aluminium garnet (Nd‐YAG) laser. Multiphysics simulation is conducted to simulate the temperature distribution in coatings and substrates during TLC processing. X‐ray energy dispersion spectrum is used to measure the chemical composition of HAp coatings after TLC process. Pull‐out tests are conducted to measure the interfacial strength between the HAp coating and Ti substrate. Cell culture study is conducted to qualitatively evaluate the biocompatibility after TLC of HAp particles. These results show that TLC processing will open new ways of producing biocompatible bioceramic coatings with controlled thickness, and at low processing temperature. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
The effect of scaffold architecture on properties of direct 3D fiber deposition of porous Ti6Al4V for orthopedic implants
Authors: Li J. P. , de Wijn J. R. , van Blitterswijk C. A. , de Groot K. 
Published: January 2009

Abstract3D porous Ti6Al4V scaffolds were directly fabricated by a rapid prototyping technology, 3D fiber deposition (3DF). In this study, scaffolds with different structures were fabricated by changing fiber spacing and fiber orientation. The influence of different architectures on mechanical properties and permeability of the scaffold were investigated. Mechanical analysis revealed that compressive strength and E‐modulus increase with decreasing the porosity. Permeability measurements showed that not only the total porosity but also the porous structure can influence the permeability. 3DF was found to provide good control and reproducibility of the desired degree of porosity and the 3D structure. Results of this study demonstrate that the 3DF of Ti6Al4V give us flexibility and versatility to fabricate and improve scaffolds to better mimic the architecture and properties of natural bone and meet the requirements of bone graft substitutes and orthopedic and dental implants. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Bioactivation of collagen matrices through sustained VEGF release from PLGA microspheres
Authors: Borselli Cristina , Ungaro Francesca , Oliviero Olimpia , d'Angelo Ivana , Quaglia Fabiana , La Rotonda Maria I. , Netti Paolo A. 
Published: January 2009

AbstractThe success of any tissue engineering implant relies upon prompt vascularization of the cellular construct and, hence, on the ability of the scaffold to broadcast specific activation of host endothelium and guide vessel ingrowth. Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator, and if released in a controlled manner it may enhance and guide scaffold vascularization. Therefore, the aim of this work was to realize a scaffold with integrated depots able to release VEGF in a controlled rate and assess the ability of this scaffold to promote angiogenesis. VEGF‐loaded poly(lactide‐co‐glycolide) (PLGA) microspheres were produced and included in a collagen scaffold. The release of VEGF from microspheres was tailored to be sustained over several weeks and occurred at a rate of ∼0.6 ng/day per mg of microspheres. It was found that collagen scaffolds bioactivated with VEGF‐loaded microspheres strongly enhanced endothelial cell activation and vascular sprouting both in vitro and in vivo as compared with a collagen scaffold bioactivated with free VEGF. This report demonstrates that by finely tuning VEGF release rate within a polymeric scaffold, sprouting of angiogenic vessels can be guided within the scaffolds interstices as well as broadcasted from the host tissues. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
In vivo performance of selective electron beam‐melted Ti‐6Al‐4V structures
Authors: Ponader Sabine , von Wilmowsky Cornelius , Widenmayer Martin , Lutz Rainer , Heinl Peter , Körner Carolin , Singer Robert F. , Nkenke Emeka , Neukam Friedrich W. , Schlegel Karl A. 
Published: January 2009

AbstractHighly porous titanium structures are widely used for maxillofacial and orthopedic surgery because of their excellent mechanical properties similar to those of human bone and their facilitation of bone ingrowth. In contrast to common methods, the generation of porous titaniumproducts by selective electron beam melting (SEBM), an additive manufacturing technology, overcomes difficulties concerning the extreme chemical affinity of liquid titanium to atmospheric gases which consequently leads to strongly reduced ductility of the metal. The purpose of this study was to assess the suitability of a smooth compact and a porous Ti‐6Al‐4V structure directly produced by the SEBM process as scaffolds for bone formation. SEBM‐processed titanium implants were placed into defects in the frontal skull of 15 domestic pigs. To evaluate the direct contact between bone and implant surfaces and to assess the ingrowth of osseous tissue into the porous structure, microradiographs and histomorphometric analyses were performed 14, 30, and 60 days after surgery. Bone ingrowth increased significantly during the period of this study. After 14 days the most outer regions of the implants were already filled with newly formed bone tissue (around 14%). After 30 days the bone volume inside the implants reached almost 30% and after 60 days abundant bone formation inside the implants attained 46%. During the study only scarce bone–implant contact was found around all implants, which did not exceed 9% around compact specimens and 6% around porous specimens after 60 days. This work demonstrates that highly porous titanium implants with excellent interconnectivity manufactured using the SEBM method are suitable scaffolds for bone ingrowth. This technique is a good candidate for orthopedic and maxillofacial applications. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Fibrin‐based tissue engineering scaffolds enhance neural fiber sprouting and delay the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury
Authors: Johnson Philip J. , Parker Stanley R. , Sakiyama‐Elbert Shelly E. 
Published: January 2009

AbstractThe purpose of this study was to evaluate the effects of fibrin scaffolds on subacute rat spinal cord injury (SCI). Long–Evans rats were anesthetized and underwent a dorsal hemisection injury; two weeks later, the injury site was re‐exposed, scar tissue was removed, and a fibrin scaffold was implanted into the wound site. An effective method for fibrin scaffold implantation following subacute SCI was investigated based on the presence of fibrin within the lesion site and morphological analysis 1 week after implantation. Prepolymerized fibrin scaffolds were found to be present within the lesion site 1 week after treatment and were used for the remainder of the study. Fibrin scaffolds were then implanted for 2 and 4 weeks, after which spinal cords were harvested and evaluated using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans. Compared with untreated control, the fibrin‐treated group had significantly higher levels of neural fiber staining in the lesion site at 2 and 4 weeks after treatment, and the accumulation of glial fibrillary acidic protein (GFAP) positive reactive astrocytes surrounding the lesion was delayed. These results show that fibrin is conducive to regeneration and cellular migration and illustrate the advantage of using fibrin as a scaffold for drug delivery and cell‐based therapies for SCI. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Enzymatically crosslinked porous composite matrices for bone tissue regeneration
Authors: Ciardelli Gianluca , Gentile Piergiorgio , Chiono Valeria , Mattioli‐Belmonte Monica , Vozzi Giovanni , Barbani Niccoletta , Giusti Paolo 
Published: January 2009

AbstractThree‐dimensional porous hydroxyapatite/collagen (HA/Coll) composites with a random pore structure were obtained by freeze‐drying and crosslinked by an enzymatic treatment using microbial transglutaminase (mTGase). The procedure resulted in improved mechanical strength and thermal stability of the scaffolds. The scaffolds were characterized in terms of their stability (Coll release, swelling, collagenase‐mediated degradation), thermal properties (thermogravimetric analysis, differential scanning calorimetry), mechanical behavior under compression and cell compatibility. Enzymatic treatment stabilized the sponges to water vapors, with measurable swelling ratio between 100% for HA/Coll/mTGase 0/100 to 5% for HA/Coll/mTGase 80/20. Weight loss in water due to Coll release was between 2 and 10% in mTGase‐crosslinked samples and decreased with increasing HA content. Cultures of MG63 osteoblast‐like cells and human umbilical vein endothelial cells (HUVEC) showed good adhesion and proliferation on the scaffolds, good viability (through MTT test, 100–150% of control), and good differentiation (alkaline phosphatase, up to 40 UI/L with respect to 35 UI/L for control). © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
In vivo response of novel calcium phosphate‐mullite composites: Results up to 12 weeks of implantation
Authors: Nath Shekhar , Basu Bikramjit , Mohanty Mira , Mohanan P. V. 
Published: January 2009

AbstractIn this paper, the in vivo response, in particular, the histocompatibility of newly developed CaP‐mullite composites is reported. In the present experiments, the bioceramic implants were inserted in the long bones of healthy rabbits according to standard protocols (ISO‐10993) and the tissue response was studied at different time intervals of up to 12 weeks. Ultra high‐molecular weight polyethylene (UHMWPE) was used as control samples. The postimplant bone‐material interfaces were analyzed by staining of histological sections, following bone tissue histopathology protocols. The interface zones were critically observed by fluorescent optical microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Importantly, no inflammation, necrosis was observed during this tenure. New bone formation was observed at all the implantation time intervals (1–12 weeks). However, the bone integrity with the material was increased after 12 weeks of implantation. Although macrophages and fibrous tissue were present during the first week of implantation, they were not observed on histology sections after 12 weeks postimplantation. More importantly, foci of chondrocytes could be observed after 12 weeks of implantation. Remodeling of Haversian canal was observed at the interfacial area of natural bone and implant material. All the observations were assessed critically to analyze the in vivo biocompatibility of this novel composite material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Pivoting system fracture in a bileaflet mechanical valve: A case report
Authors: Zhang Hongbo , Deng Xiaoyan , Cianciulli Tomás Francisco , Zhang Ze , Chappard Daniel , Lax Jorge Alberto , Saccheri Maria Cristina , Redruello Hector Jorge , Jordana Jorge Luis , Prezioso Horacio Alberto , King Martin , Guidoin Robert 
Published: January 2009

AbstractA leaflet escape occurred in a low profile bileaflet mechanical prosthesis manufactured by TRI‐Technologies that had been implanted for 3 years in the mitral position of a 32 year old patient. The escaped leaflet had embolized and was subsequently located by an abdominal computerized axial tomography scan and ultrasound in the terminal portion of the aortic bifurcation. The embolized leaflet was removed 3 months after valve replacement surgery. In an attempt to determine the cause of the escape the retrieved embolized leaflet was investigated. Techniques employed included visual examination aided by stereo‐microscopy, x‐ray imaging and scanning electron microscopy. One of the ears had fractured and was missing from the leaflet. Chipping was observed at the leaflet ear position on both the inflow and outflow surfaces. Visual and SEM observations found fractographic river‐lines that indicated an apparent origin at the inflow surface of the ear nearest to the straight ‘B‐datum’ line or coaptation edge. The origin seemed to be in the radius between the leaflet ear and the leaflet body. SEM observation of the remaining intact ear showed wear marks on both the inflow and outflow sides of the leaflet ear that corresponded to the suspected origin of fracture. It is believed that the use of boron alloyed pyrolytic carbon material and the leaflet's homogeneous monolithic structural design were factors that contributed to this adverse event. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Postimplantation pressure testing and characterization of laser bonded glass/polyimide microjoints
Authors: Mian Ahsan , Sultana Taslema , Georgiev Daniel , Witte Reiner , Herfurth Hans , Auner Greg , Newaz Golam 
Published: January 2009

AbstractThe stability of the laser bonded titanium coated glass/polyimide microjoints were studied in vivo by implanting on a rat brain surface for 10 days. In the current state, the strength of the joints were measured by a specially designed instrument called “pressure test” equipment where the samples were subjected to a variable pressure load (using high pressure nitrogen) controlled by a pressure regulator. The strength of the joints seems to degrade by about 28% as a result of soaking in rat brain. The bond degradation in rat brain implants is similar compared with those soaked in artificial cerebrospinal fluid (CSF) solution. Polyimide uptakes water through existing pores in it and also water gets in the joint region through the edges of the samples. Water might have caused oxidation of the chemical bonds which are thought to have formed by the laser fabrication process. A separate set of samples were created using same parameters for testing the hermeticity of the laser bonds. The samples were also exposed to rat brain CSF and were tested for hermiticity at the end of 10 days exposure time. It was observed that the implanted samples retained their hermeticity although the bond strength degraded by about 28%. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009