bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic analysis of TGFβ proteomics revealed involvement of Plag1/CNK1/RASSF1A/Src network in TGFβ1‐dependent activation of Erk1/2 and cell proliferation
Authors: Bhaskaran Nimesh , Souchelnytskyi Serhiy 
Published: September 2008

AbstractTransforming growth factor‐β (TGFβ) is a key regulator of cell proliferation, death, migration, and differentiation. To explore mechanisms of TGFβ action, we performed systemic analysis of functional dependencies between 153 proteins which changed their expression and synthesis upon treatment of human breast epithelial cells with TGFβ1. We found that TGFβ1 initiated signaling via a scale‐free network of proteins which affect primary cellular metabolism, stress response, signal transduction, transport, transcription, cytoskeleton, and cell death. Multiple inputs into each functional domain were observed, emphasizing robustness of TGFβ1 signaling. Network analysis demonstrated importance of a Plag1/CNK1/RASSF1A/Src‐dependent prozone effect, as a systemic feature which is crucial for TGFβ1‐dependent activation of Erk1/2 and regulation of cell proliferation. We showed that the balance between Plag1, CNK1, RASSF1A and Src defined whether TGFβ1 will stimulate, inhibit or will have no effect on a long‐term activation of Erk1/2 and subsequent TGFβ1 inhibitory or stimulatory effect on cell proliferation. This is the first demonstration of importance of systemic features for incorporation of Erk1/2 activation into TGFβ1 signaling.
Intracellular signaling pathways regulating radioresistance of human prostate carcinoma cells
Authors: Skvortsova Ira , Skvortsov Sergej , Stasyk Taras , Raju Uma , Popper Bela‐Andre , Schiestl Bernhard , von Guggenberg Elisabeth , Neher Andreas , Bonn Guenther K. , Huber Lukas A. , Lukas Peter 
Published: September 2008

AbstractRadiation therapy plays an important role in the management of prostate carcinoma. However, the problem of radioresistance and molecular mechanisms by which prostate carcinoma cells overcome cytotoxic effects of radiation therapy remains to be elucidated. In order to investigate possible intracellular mechanisms underlying the prostate carcinoma recurrences after radiotherapy, we have established three radiation‐resistant prostate cancer cell lines, LNCaP‐IRR, PC3‐IRR, and Du145‐IRR derived from the parental LNCaP, PC3, and Du145 prostate cancer cells by repetitive exposure to ionizing radiation. LNCaP‐IRR, PC3‐IRR, and Du145‐IRR cells (prostate carcinoma cells recurred after radiation exposure (IRR cells)) showed higher radioresistance and cell motility than parental cell lines. IRR cells exhibited higher levels of androgen and epidermal growth factor (EGF) receptors and activation of their downstream pathways, such as Ras‐mitogen‐activated protein kinase (MAPK) and phosphatidyl inositol 3‐kinase (PI3K)‐Akt and Jak‐STAT. In order to define additional mechanisms involved in the radioresistance development, we determined differences in the proteome profile of parental and IRR cells using 2‐D DIGE followed by computational image analysis and MS. Twenty‐seven proteins were found to be modulated in all three radioresistant cell lines compared to parental cells. Identified proteins revealed capacity to interact with EGF and androgen receptors related signal transduction pathways and were involved in the regulation of intracellular routs providing cell survival, increased motility, mutagenesis, and DNA repair. Our data suggest that radioresistance development is accompanied by multiple mechanisms, including activation of cell receptors and related downstream signal transduction pathways. Identified proteins regulated in the radioresistant prostate carcinoma cells can significantly intensify activation of intracellular signaling that govern cell survival, growth, proliferation, invasion, motility, and DNA repair. In addition, such analyses may be utilized in predicting cellular response to radiotherapy.
Preparation of exfoliated polyacrylic clay nanocomposites with high loading: An investigation into the intercalation of ammonium‐terminated polyacrylic acid and polyacrylates
Authors: Wu Tongfei , Xie Tingxiu , Yang Guisheng 
Published: September 2008

AbstractLow‐molecular‐weight polyacrylic acid with amine chain end used as a macromolecular intercalating agent was synthesized by radical polymerization using 2‐aminoethanethiol hydrochloride as chain transfer agent. Three polyacrylates (sodium polyacrylate‐t‐NH, calcium polyacrylate‐t‐NH, and zinc polyacrylate‐t‐NH) were prepared by neutralization from this polyacrylic acid using sodium hydroxide, zinc oxide, and calcium hydroxide as alkalies. The intercalation of ammonium‐terminated polyacrylic acid and polyacrylate was investigated by viscosity measurement, XRD, and TEM. Using this ammonium‐terminated polyacrylic acid as the intercalating agent, exfoliated polyacrylic acid/clay and polyacrylate/clay composites with a clay loading of ∼30 and 20 wt %, respectively, were prepared through the evaporation of solvent from their clay suspensions. The thermal degradation of polyacrylic acid/clay and polyacrylate/clay composites was also studied by TGA. © 2008 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 46: 2335–2340, 2008
An evidence‐based review of yoga as a complementary intervention for patients with cancer
Authors: Smith Kelly B. , Pukall Caroline F. 
Published: September 2008

AbstractObjective: To conduct an evidence‐based review of yoga as an intervention for patients with cancer. Specifically, this paper reviewed the impact of yoga on psychological adjustment among cancer patients.Methods: A systematic literature search was conducted between May 2007 and April 2008. Data from each identified study were extracted by two independent raters; studies were included if they assessed psychological functioning and focused on yoga as a main intervention. Using a quality rating scale (range = 9–45), the raters assessed the methodological quality of the studies, and CONSORT guidelines were used to assess randomized controlled trials (RCTs). Effect sizes were calculated when possible. In addition, each study was narratively reviewed with attention to outcome variables, the type of yoga intervention employed, and methodological strengths and limitations.Results: Ten studies were included, including six RCTs. Across studies, the majority of participants were women, and breast cancer was the most common diagnosis. Methodological quality ranged greatly across studies (range = 15.5–42), with the average rating (M = 33.55) indicating adequate quality. Studies also varied in terms of cancer populations and yoga interventions sampled.Conclusions: This study provided a systematic evaluation of the yoga and cancer literature. Although some positive results were noted, variability across studies and methodological drawbacks limit the extent to which yoga can be deemed effective for managing cancer‐related symptoms. However, further research in this area is certainly warranted. Future research should examine what components of yoga are most beneficial, and what types of patients receive the greatest benefit from yoga interventions. Copyright © 2008 John Wiley & Sons, Ltd.
Measuring attachment security in patients with advanced cancer: psychometric properties of a modified and brief Experiences in Close Relationships scale
Authors: Lo Christopher , Walsh Andrew , Mikulincer Mario , Gagliese Lucia , Zimmermann Camilla , Rodin Gary 
Published: September 2008

AbstractObjective: Attachment security has been identified as an important buffer of distress in patients with cancer and other medical illnesses but current measures have not been adapted for this population who may be older, in long‐term stable relationships, and suffering from considerable disease burden. This study reports on (1) the psychometric properties of a modified 36‐item Experiences in Close Relationships scale (ECR), adapted for this population; and (2) the validity of a brief 16‐item version of our modified scale.Methods: A modified ECR (ECR‐M36) was constructed by rephrasing relevant items to refer more generally to people with whom one feels close, instead of specifically in relation to one's romantic partner(s). Patients with metastatic gastrointestinal (GI) and lung cancer completed the ECR‐M36 and other scales tapping self‐esteem, social support, and depressive symptoms on two occasions within a period of 4–6 months. Based on factor analyses of the ECR‐M36, 16 items were selected to form a brief measure (ECR‐M16).Results: Factor analyses of both ECR forms revealed a higher‐order factor structure in which four first‐order factors (Worrying about Relationships, Frustration about Unavailability, Discomfort with Closeness, Turning Away from Others) loaded onto two second‐order factors tapping Attachment Anxiety and Avoidance. Both ECR forms were reliable and valid.Conclusion: The ECR‐M36 and ECR‐M16 are good measures of attachment orientations for use with medically ill, older populations. Copyright © 2008 John Wiley & Sons, Ltd.
Copper‐filled macroporous Si and cavity underneath for microchannel heat sink technology
Authors: Zacharatos F. , Nassiopoulou A. G. 
Published: September 2008

AbstractThermal management in ICs becomes essential as integration density and total power consumption increase. The use of microchannels in high power density electronics cooling is a well‐known technique for heat transfer. In this work we developed Cu‐filled macroporous Si channels with a Cu‐filled cavity underneath, which may be used as heat sinks in high power density electronics cooling. Macroporous Si is formed by electrochemical dissolution of bulk Si, while pore filling with copper is achieved by electro‐deposition. Using appropriate design, the resulting composite material may be fabricated on selected areas on the silicon substrate for use as heat sink on Si. The surface area is defined by patterning. The macroporous Si structure is composed of either randomly distributed pores or pores arranged in two‐dimensional (2‐D) arrays, fabricated by pre‐patterning the Si surface before anodization so as to form pore initiation pits. The pore size in this work was 5μm, while the porous layer and the cavity underneath had both a thickness of 40 μm. Copper deposition proceeds first by filling the micro‐cavity underneath the porous layer. This is achieved by linearly increasing the applied potential during electro‐deposition. After full Cu‐filling of the cavity, pore filling starts from the bottom of each pore and proceeds laterally, while no nucleation takes place on pore wall. In this way, homogeneous copper wires within pores may be fabricated. The Cu/Si composite material is appropriate for forming channels with improved heat transfer within the Si wafer. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A thermal vacuum sensor fabricated on plastic substrate – Study in various operation modes
Authors: Petropoulos A. , Kaltsas G. , Nassiopoulou A. G. 
Published: September 2008

AbstractThis work presents the characterization of a thermal vacuum sensor, directly integrated on a printed circuit board (PCB) substrate. The sensor consists of a thin platinum strip, in direct electrical contact with the patterned Cu tracks of the PCB. The platinum resistor that resides on a thick SU‐8 layer on the PCB, acts as both heating and temperature sensing element. Both PCB substrate and SU‐8 have low thermal conductivity values, therefore ensuring a high degree of thermal isolation. Under electrical current flow through the Pt heater, an elevated temperature field is developed in its vicinity due to Joule heating. The overall heat exchange rate is a function of the surrounding gas pressure in certain pressure regimes due to the contribution of different heat transfer mechanisms. The determination of heat transfer allows for the extraction of the corresponding vacuum value. The sensor operation was tested in a pressure range from 103to 10–5mbar for three different operation modes, namely that of constant current (CI), constant temperature (CT) and constant power (CP). (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effect of guava (Psidium guajava Linn.) leaf soluble solids on glucose metabolism in type 2 diabetic rats
Authors: Shen Szu‐Chuan , Cheng Fang‐Chi , Wu Ning‐Jung 
Published: September 2008

AbstractThis study investigated the effect of aqueous and ethanol soluble solid extracts of guava (Psidium guajava Linn.) leaves on hypoglycemia and glucose metabolism in type 2 diabetic rats. Low‐dose streptozotocin (STZ) and nicotinamide were injected into Sprague‐Dawley (SD) rats to induce type 2 diabetes. Acute and long‐term feeding tests were carried out, and an oral glucose tolerance test (OGTT) to follow the changes in plasma glucose and insulin levels was performed to evaluate the antihyperglycemic effect of guava leaf extracts in diabetic rats.The results of acute and long‐term feeding tests showed a significant reduction in the blood sugar level in diabetic rats fed with either the aqueous or ethanol extract of guava leaves (p < 0.05). Long‐term administration of guava leaf extracts increased the plasma insulin level and glucose utilization in diabetic rats. The results also indicated that the activities of hepatic hexokinase, phosphofructokinase and glucose‐6‐phosphate dehydrogenase in diabetic rats fed with aqueous extracts were higher than in the normal diabetic group (p < 0.05). On the other hand, diabetic rats treated with the ethanol extract raised the activities of hepatic hexokinase and glucose‐6‐phosphate dehydrogenase (p < 0.05) only. The experiments provided evidence to support the antihyperglycemic effect of guava leaf extract and the health function of guava leaves against type 2 diabetes. Copyright © 2008 John Wiley & Sons, Ltd.
Dynamic relay deployment for disaster area wireless networks
Authors: Guo Wenxuan , Huang Xinming , Liu Youjian 
Published: September 2008

AbstractThis paper investigates the disaster area communication system using relay‐assisted wireless network for first responders as mobile nodes (MNs). Firstly, a novel mobility model is proposed to describe the movement pattern of MNs within a large disaster area. Secondly, we study the relay management problem of finding a minimum number of relay nodes (RNs) and their dynamic locations to cover all the MNs within the disaster area. A square disk cover (SDC) problem is formulated and three different algorithms, including the two‐vertex square covering (TVSC) algorithm, the circle covering algorithm and the binary integer programming (BIP) algorithm, are proposed to solve the SDC problem. Simulation results are presented to validate the mobility model and compare the algorithms with respect to computational complexity and worst case performance ratio. Copyright © 2008 John Wiley & Sons, Ltd.
Co‐transplantation of bFGF‐expressing amniotic epithelial cells and neural stem cells promotes functional recovery in spinal cord‐injured rats
Authors: Meng Xiao‐ting , Li Chen , Dong Zhi‐yong , Liu Jia‐mei , Li Wei , Liu Ying , Xue Hui , Chen Dong 
Published: September 2008

AbstractWe have previously demonstrated that amniotic epithelial cells (AECs) can enhance survival and neural differentiation of neural stem cells (NSCs) when co‐cultured in basal media. In addition, the presence of basic fibroblast growth factor (bFGF) enhances this AEC function. The aim of the present study was to extend those findings and investigate whether AECs modified with the bFGF gene will also enhance NSCs survival and neural differentiation in vivo and promote repair of the injured spinal cord. Female Wistar rats were used for a contusive spinal cord injury (SCI) model. Contusive SCIs were induced using a weight‐drop device at levels T9–T11. Seven days following contusion, rats received grafts of NSCs only, NSCs with AECs/pLEGFP‐hbFGF, or NSCs with AECs/pLEGFP‐C1 into the injured region. Significant locomotor improvement was observed in the NSCs/AECs co‐graft group beginning at 3 weeks compared with the NSCs or NaCl only groups. These results were confirmed and extended in an electrophysiological analysis. An immunohistological analysis revealed that AECs/pLEGFP‐hbFGF promoted the survival (vs NaCl group: 194 ± 9.17 vs 103.6 ± 13.05) and neural differentiation (vs NaCl group: 14.24 ± 1.11 vs 7 ± 0.63) of co‐transplanted NSCs. We also confirmed that AECs could promote the survival of host neurons. These results suggest that AECs/pLEGFP‐hbFGF improve the NSCs survival and differentiation microenvironment and may be useful as a source of sustained trophic supported to improve NSCs differentiation into neurons in vivo. These findings suggest that a cograft of AECs/pLEGFP‐hbFGF and NSCs may have benefits for SCI.
Neuronal characteristics of amniotic fluid derived cells after adenoviral transformation
Authors: Arnhold S. , Post C. , Glüer S. , Hoopmann M. , Wenisch S. , Volpers C. , Addicks K. 
Published: September 2008

AbstractEfficient transformation of primary human amniocytes by E1 gene functions of human adenovirus serotype 5 (Ad5) yield in stable cell lines, which exhibit morphological features of epithelial like cells. A thorough investigation using immunocytochemistry confirmed the expression of epithelial cell markers. The analysis also revealed the expression of neuronal and glial marker proteins, such as nestin, vimentin, A2B5 and GFAP. Using RT‐PCR, transcripts of the neurotrophic factors nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), glial cell line derived neurotrophic factor (GDNF), and neurotrophin 3 (NT‐3) could be detected. Neurotrophic factors could also be detected in the cell culture supernatants of transformed amniocytes. In line with previous experimental data on a human Ad5 E1‐transformed embryonal kidney cell line (HEK‐293), the results suggest a co‐expression of epithelial and neuronal marker proteins in E1‐transformed human amniotic fluid derived cells and thus a preferential transformation into neuronal‐like cells.
Detection of antibodies againstFasciola hepaticain cirrhotic patients from Peru
Authors: Marcos L.A. , Bussalleu A. , Terashima A. , Espinoza J.R. 
Published: September 2008

AbstractThe prevalence ofFasciola hepaticainfection, in endemic countries, in patients with established cirrhosis is unknown. We hypothesized that, in endemic countries, the presence of fascioliasis may be detected in a serum pool of cirrhotic patients. Forty-four previously stored serum samples of patients with established liver cirrhosis, in the Hospital Nacional Cayetano Heredia in Lima, Peru, were collected from 1998 to 2003 and assessed for hepatitis B, C and fascioliasis antibodies (Fas2 ELISA). Hepatitis B surface antigen (HBsAg) was positive in 8.8% (n = 34), hepatitis B core antibody (anti-HBc) in 32.5% (n = 34), hepatitis C antibodies (anti-HCV) in 9.1% (n = 33), and 9.1% (n = 44) were Fas2 ELISA positive. This disease is an example of an emerging tropical infection which can be present in chronic liver diseases, requiring greater clinician awareness especially in endemic rural areas. Further clinical studies are warranted.
Bald'sLeechbookand cultural interactions in Anglo-Saxon England
Authors: Cameron M. L. 
Published: September 2008

The Old English medical records are rich in materials which contain evidence for contacts between the Anglo-Saxons and other cultures. For example, in 1945 Howard Meroney collected the various loan translations of Irish words found in the medical charms in theLeechbook, Lacnungaand other Old English texts. It is an interesting exercise to speculate on how Irish charms such as these, in Old Irish, got into the Old English medical repertory in their pristine form, whereas most of the Latin medical charms were translated straightway into English. It is customary to suppose that the Anglo-Saxons picked them up from Irish teachers in their monasteries, but there may be other explanations. Recently, while I was reading theHisperica famina(in Michael Herren's translation), I came across references to young ‘visitors’, students who wandered about the Irish countryside begging for food and shelter among the country people, with whom they had difficulty communicating; as one of them is made to say:Who will ask these possessorsto grant us their sweet abundance?For an Ausonian chain binds me;hence I do not utter good Irish speech.
Oaks, ships, riddles and the Old EnglishRune Poem
Authors: Sorrell Paul 
Published: September 2008

The Holme Riddles are a collection of 144 English riddles preserved in London, British Library, Harley 1960. Although the manuscript dates from the mid-seventeenth century, some of these riddles are apparently rooted in ancient Germanic tradition. One of these, an ancient ‘world-riddle’ according to the editor, is a version of the ‘oak-ship’ riddle:Q. wnj lived j fed the liveing now j am dead j beare the live[in]g & with swift speed j walk ourthe liveingA. a ship mad[e] of oake groweing feeds hogs with acorns now b[e]ars men & swims ourfishes.
Regula canonicorum or Regula monasterialis uitae? The Rule of Chrodegang and Archbishop Wulfred's reforms at Canterbury
Authors: Langefeld Brigitte 
Published: September 2008

Around the years 755–6 Chrodegang, bishop of Metz from 742 to 766 and metropolitan of the Austrasian church since the death of Boniface in 754, compiled a rule for the canons of his own familia in Metz. His paruum decretulum or parua institutio, as he himself called it, contained very few thoughts not already expressed about the spiritual principles and practical organization of those canons who wished to live in a quasi-monastic, celibate community. Indeed, Chrodegang himself made it abundantly clear in the preface to his Regula that he would have had no need to compile his Rule if the writings of the Church Fathers and existing ecclesiastical laws on this subject had been honoured:Si trecentorum decem et octo reliquorumque sanctorum Patrum canonum auctoritas perduraret, et clerus atque episcopus secundum eorum rectitudinis normam uiuerent, superfluum uideretur a nobis exiguis minimisque, super hac re tarn ordinate disposita aliquid retractari, et quasi quidem noui aliquid dici.
TheWest Saxon Gospelsand the gospel-lectionary in Anglo-Saxon England: manuscript evidence and liturgical practice
Authors: Lenker Ursula 
Published: September 2008

Farað witodlice and Iærað ealle þeoda and fulligeaþ hig on naman fæder and suna and þæs halgan gastes, and lærað þæt hig healdon ealle þa ðing þe ic eow behead (Matt. XXVIII.19–20).With these words at the end of the gospel according to Matthew, Jesus sends out his disciples to spread the words and deeds of the Lord to all peoples. With respect to the Anglo-Saxons, this order was impressively executed by the earliest translation of the Vulgate gospels into a vernacular, theWest Saxon Gospels (WSG). This text, from the late tenth or early eleventh century, survives in four complete manuscripts (A, B, C, Cp) and two fragments (F, L) from the Old English period and two complete manuscripts from the late twelfth century (R and its copy H).
A third Old English translation of part of Gregory's Dialogues, this time embedded in the Rule of Chrodegang
Authors: Langefeld Brigitte 
Published: September 2008

Gregory's Dialogues are a hitherto unnoticed source of the final chapter of the enlarged version of the Rule of Chrodegang of Metz. The chapter in question, no. 84 or 86 depending on the recension of the Latin text, is preserved in the following manuscripts (the letters in brackets are the sigla used for these manuscripts throughout this article):Paris, Bibliothèque Nationale, lat. 1535 (P), 113V–149V. Second quarter of the ninth century, possibly written at Fécamp. Latin text only, 86 chapters.