bluuebunny/crossref_metadata_2025_split huggingface dataset:
The autocorrelation matrix probing biochemical relationships after metabolic fingerprinting with CE
Authors: Angulo Santiago , García‐Pérez Isabel , Legido‐Quigley Cristina , Barbas Coral 
Published: March 2009

AbstractFingerprinting together with statistical analysis is often employed to compare samples in metabonomic studies of a disease. Correlation algorithms can aid by extracting information based on the variation patterns of key metabolites. This information can be linked to metabolite identification or to specific up/down‐regulated biochemical pathways. Matlab‐based software employing the Pearson's correlation algorithm was applied to urine electropherograms from 20 mice infected with the schistosoma parasite. The fingerprints were the sum of electropherograms analysed with normal and reverse polarity, in two different modes MEKC and CZE and with two different capillaries (uncoated and polyacrylamide coated) to provide a broad picture of the samples. Hippurate, a metabolite that was depleted in the infected group and is present in both polarities, was chosen as a test variable; it correlated with itself to a p value of <0.000. Phenylacetylglycine, a metabolite shown as over expressed in the disease, was positively correlated to three metabolites in its same pathway with a correlation coefficient of 0.7 and p<0.000 to phenylalanine, 0.7 and p<0.000 to 2‐hydroxyphenylacetic and 0.55 and p<0.003 to phenylacetate. The study shows that the autocorrelation matrix is able to provide extra information from data files acquired by CE analyses. It underlined an up‐regulated metabolic path by association in the schistosoma infection model.
Untargeted metabolite analysis of healthy and Huanglongbing‐infected orange leaves by CE‐DAD
Authors: Cevallos‐Cevallos Juan Manuel , Rouseff Russell , Reyes‐De‐Corcuera José I. 
Published: March 2009

AbstractHuanglongbing (HLB) is considered the most destructive bacterial citrus disease worldwide. Early detection of HLB is crucial for minimizing its spread. CE was used for the discovery of potential biomarkers for HLB. Optimization of extraction and separation allowed resolving 24 compounds of which 6 were present in significantly higher (p<0.05) concentrations in HLB‐infected samples collected monthly for 6 months during the 2007–2008 season. Three of these compounds were identified by mobility and UV spectra as hesperidin, naringenin, and quercetin with mean increase in concentration of 154, 555, and 467%, respectively, above that in healthy leaves. Results support the potential of CE‐DAD for untargeted plant metabolomic analysis. CZE, NACE, and MEEKC were compared for metabolic differentiation of healthy and HLB‐infected citrus leaves. CZE in a semi‐aqueous BGE solution consisting of 8.5 mM of sodium borate (pH 9.3), 15% ACN, and 9% 1‐butanol yielded the best peak separation with detection at 190 nm.
Comparison of 1‐D and 2‐D LC MS/MS methods for proteomic analysis of human serum
Authors: Gilar Martin , Olivova Petra , Chakraborty Asish B. , Jaworski Aleksander , Geromanos Scott J. , Gebler John C. 
Published: March 2009

Abstract1‐D and 2‐D LC methods were utilized for proteome analysis of undepleted human serum. Separation of peptides in 2‐D LC was performed either with strong cation exchange (SCX)‐RP chromatography or with an RP–RP 2‐D LC approach. Peptides were identified by MS/MS using a data‐independent acquisition approach. A peptide retention prediction model was used to highlight the potential false‐positive peptide identifications. When applying selected data filtration, we identified 52 proteins based on 316 peptides in serum in 1‐D LC setup. One hundred and eighty‐four proteins/1036 peptides and 142 proteins/905 peptides were identified in RP–RP and SCX‐RP 2‐D LC, respectively. The performance of both 2‐D LC methods for proteomic analysis is critically compared.
Lichen extracts as raw materials in perfumery. Part 2: treemoss
Authors: Joulain Daniel , Tabacchi Raphaël 
Published: March 2009

AbstractThis is a comprehensive review of extracts from the lichen Pseudevernia furfuracea (treemoss) that are used in the fragrance industry. Qualitative and quantitative analytical aspects are critically reviewed and the results are compared to those of the related oakmoss extracts. It is shown that more than 90 constituents have been identified so far in treemoss extracts, including 42 depsides, depsidones or depside‐derived compounds, and 42 triterpenes or steroids. Constituents of certain host trees, mainly Pinus species, generate specific analytical and toxicological issues which need to be considered in addition to those related to the known degradation products of lichen compounds. A new classification of lichen extracts used as raw materials in fragrance compounding is proposed. Copyright © 2009 John Wiley & Sons, Ltd.
Ordered Mesoporous Carbons as Catalyst Support for PEM Fuel Cells
Authors: Ambrosio E. P. , Dumitrescu M. A. , Francia C. , Gerbaldi C. , Spinelli P. 
Published: March 2009

AbstractPreliminary results on Pt‐based electrocatalysts prepared with CMK3 mesoporous carbons are presented. The electro‐catalytic performance towards the oxygen reduction reaction (ORR) was compared to that of commercial Pt/C‐Vulcan catalysts with the same Pt content. Polarisation tests were carried out with a single PEM fuel cell containing the Pt/OMC cathodes. The analysis of these tests, based on a semi‐empirical determination of the various polarisation terms, showed an interesting catalytic activity for ORR for the cathodes prepared with mesoporous carbon supports. The preliminary results indicate some critical aspects which need further investigation to improve the ohmic resistance limitation of these materials. Particularly, the variation of the grain size distribution with the Pt content and the effective distribution of Nafion® on the OMC surface should be considered.
Depression status as a reliable predictor of functional decline among Japanese community‐dwelling older adults: a 12‐year population‐based prospective cohort study
Authors: Iwasa Hajime , Yoshida Yuko , Kumagai Shu , Ihara Kazushige , Yoshida Hideyo , Suzuki Takao 
Published: March 2009

AbstractObjectiveThis study aimed to examine a longitudinal relationship between depression status and functional decline among Japanese community‐dwelling older adults, using a 12‐year population‐based, prospective cohort study design.MethodA total of 710 men and women, aged 65 years and over, participated in the study. Katz's Index and the Tokyo Metropolitan Institute of Gerontology Index of Competence were used to measure the functional capacities of basic activities of daily living (BADL) and higher‐level competence, respectively. For the purpose of analysis, a decline in each subscale of functional capacity during the follow‐up period were used as outcome variables; depression status assessed by the Japanese version of the 30‐item Geriatric Depression Scale (GDS), with a cut‐off of 11, was used as an independent variable; and age, gender, education level, history of chronic disease, hospitalization, smoking, physical activity, living alone, hearing problems, physical pain, dietary habits, and usual walking speed at baseline were used as covariates.ResultsUse of the multivariate Cox proportional hazards model adjusted for potential confounders showed that depression status was significantly and independently associated with BADL decline (risk ratio (RR) = 1.46, 95% confidence interval (CI): 1.13–1.89) and with higher‐level competence decline (RR = 1.56, 95% CI: 1.18–2.04).ConclusionOur study found an independent relationship between depression status and longitudinal change in functional capacity among community‐dwelling older individuals, suggesting that depression status is a reliable predictor of functional decline (both of BADL decline and higher‐level competence decline) in older adults. Copyright © 2009 John Wiley & Sons, Ltd.
Neural cell adhesion molecule expression: No correlation with perineural invasion in cutaneous squamous cell carcinoma of the head and neck
Authors: Solares C. Arturo , Brown Ian , Boyle Glen M. , Parsons Peter G. , Panizza Benedict 
Published: March 2009

AbstractBackground.Perineural invasion (PNI) in cutaneous squamous cell carcinoma of the head and neck (CSCCHN) is associated with decreased survival, particularly in patients with clinical signs of cranial nerve involvement. There is evidence to indicate that neural cell adhesion molecule (N‐CAM) confers capability of PNI. We analyzed our own patient population to determine if N‐CAM predicted clinical PNI in CSCCHN.Methods.Tissue from patients with CSCCHN and clinical PNI, who underwent surgery between 1998 and 2005, was immunostained for N‐CAM. In addition, non‐PNI CSCCHN and normal nerve sections were also stained. A section of neuroendocrine tumor was included in each slide as a positive control. In addition, most of the sections also had an “inbuilt control” in the CD56 positive natural killer T cells that formed part of the inflammatory reaction to the tumors.Results.Tissue was available from 14 patients with CSCCHN and clinical PNI. The analysis was carried out in 14 patients without PNI and 4 normal nerves. N‐CAM was not expressed in any of our PNI CSCCHN specimens or non‐PNI controls. It was strongly expressed in the neuroendocrine tumors and positive in‐built controls, as well as in normal nerve tissue.Conclusion.N‐CAM expression did not predict neurotropism in our patient population. Additional studies are required to identify the cell surface markers expressed by CSCCHN which confer neurotropism capabilities. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
A new, simple maxillary‐sparing tongue depressor for external mandibular radiotherapy: A case report
Authors: Bodard Anne‐Gaëlle , Racadot Séverine , Salino Samuel , Pommier Pascal , Zrounba Philippe , Montbarbon Xavier 
Published: March 2009

AbstractBackground.In external radiotherapy, the use of leaded intraoral devices to shield adjacent tissues is well described, but stabilization of the mandible is often realized with standard bite blocks, which do not allow a good stabilization of the mandible.Methods and Results.A 40‐year‐old patient presenting with a local recurrence of a tumor of the floor of the mouth underwent external radiotherapy. To shield the maxilla during mandibular irradiation and to ensure mandible and tongue immobility during external radiotherapy, a custom‐made device was realized. The apparatus consists of a polymethylmethacrylate resin plate and 2 wedges. Only 2 steps are needed.Conclusion.The realization is simple and cheap, the apparatus is comfortable, and modifications are easy. It can be used in all patients, except in patients with totally mandibular edentulous with severe bone resorption. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Prognostic factors for the malignant triton tumor of the head and neck
Authors: Terzic Andrej , Bode Beata , Gratz Klaus W. , Stoeckli Sandro J. 
Published: March 2009

AbstractBackground.Malignant triton tumors are rare neoplasias consisting of a malignant peripheral nerve sheath tumor with additional rhabdomyoblastic differentiation. These tumors are highly aggressive and prognosis is poor. Our aim is to describe the outcome and to identify potential prognostic factors.Methods.From 1993 to 2005, 7 patients with a malignant triton tumor of the head and neck were treated at our institution. A literature search revealed another 46 published cases. All these cases were analyzed for outcome and prognostic factors.Results.Patients with primary tumors involving the nose and paranasal sinuses have better, patients involving the neck a poor prognosis. All other locations show an intermediate course. Complete surgical removal is of crucial importance. Additional radiation or chemotherapy show little effect.Conclusion.Location of the primary tumor is a key factor for prognosis. Complete surgical removal is the only treatment associated with survival.© 2009 Wiley Periodicals, Inc. Head Neck, 2009
Larynx preservation clinical trial design: Key issues and recommendations—A consensus panel summary
Authors: Lefebvre Jean‐Louis , Ang K. Kian ,   on behalf of the Larynx Preservation Consensus Panel
Published: March 2009

AbstractBackgroundTo develop guidelines for the conduct of phase III clinical trials of larynx preservation in patients with locally advanced laryngeal and hypopharyngeal cancer.MethodsA multidisciplinary international consensus panel developed recommendations after reviewing results from completed phase III randomized trials, meta‐analyses, and published clinical reports with updates available through November 2007. The guidelines were reviewed and approved by the panel.ResultsThe trial population should include patients with T2 or T3 laryngeal or hypopharyngeal squamous cell carcinoma not considered for partial laryngectomy and exclude those with laryngeal dysfunction or age more than 70 years. Functional assessments should include speech and swallowing. Voice should be routinely assessed with a simple, validated instrument. The primary endpoint should capture survival and function. The panel created a new endpoint: laryngo‐esophageal dysfunction‐free survival. Events are death, local relapse, total or partial laryngectomy, tracheotomy at 2 years or later, or feeding tube at 2 years or later. Recommended secondary endpoints are overall survival, progression‐free survival, locoregional control, time to tracheotomy, time to laryngectomy, time to discontinuation of feeding tube, and quality of life/patient reported outcomes. Correlative biomarker studies for near‐term trials should include EGFR, ERCC‐1, E‐cadherin and β‐catenin, epiregulin and amphiregulin, and TP53 mutation.ConclusionsRevised trial designs in several key areas are needed to advance the study of larynx preservation. With consistent methodologies, clinical trials can more effectively evaluate and quantify the therapeutic benefit of novel treatment options for patients with locally‐advanced laryngeal and hypopharyngeal cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
The older‐worker‐younger‐supervisor dyad: A test of the Reverse Pygmalion effect
Authors: Collins Mary Hair , Hair, Jr. Joseph F. , Rocco Tonette S. 
Published: March 2009

AbstractAn emerging phenomenon, the older worker reporting to a much younger supervisor, is reversing the tradition that managers are older and more experienced than subordinates. These age‐related demographic changes are bringing about a role reversal in the workplace that violates established age norms, creating status incongruence in the supervisor‐subordinate dyad. This age‐reversed dyad can be better understood by examining generational differences and the effects of older workers' expectations on their younger supervisors' leadership behavior, referred to as Reverse Pygmalion. Research findings highlight demographic differences and similarities in the supervisor‐subordinate dyad, called Relational Demography. Major findings are that older workers expect less from their younger supervisors than do younger workers, and in turn older workers rate their younger supervisors' leadership behavior lower than younger workers rate their younger supervisors.
The coach‐coachee relationship in executive coaching: A field study
Authors: Baron Louis , Morin Lucie 
Published: March 2009

AbstractNumerous authors have suggested that the working relationship between coach and coachee constitutes an essential condition to the success of executive coaching. This study empirically investigated the links between the coach‐coachee relationship and the success of a coaching intervention in an organizational setting. Data were collected from two samples: 73 managers who received executive coaching for a period of eight months and 24 coaches. Results from 31 coach‐coachee dyads were analyzed. Results indicate that the coach‐coachee relationship plays a mediating role between the coaching received and development of the coachees' self‐efficacy. Findings also show four significant correlates to the coach‐coachee relationship: the coach's self‐efficacy with regard to facilitating learning and results, the coachee's motivation to transfer, his or her perception of supervisor support, and the number of coaching sessions received.
Ziprasidone as an adjuvant for clozapine‐ or olanzapine‐associated medical morbidity in chronic schizophrenia
Authors: Henderson David C. , Fan Xiaoduo , Copeland Paul M. , Sharma Bikash , Borba Christina P. , Forstbauer Sharon I. , Miley Kate , Boxill Ryan , Freudenreich Oliver , Cather Corrine , Evins Anne E. , Goff Donald C. 
Published: March 2009

AbstractObjectiveThis study sought to examine the effect of ziprasidone on olanzapine or clozapine‐associated medical morbidity such as insulin resistance, diabetes mellitus (DM) and impaired fasting glucose, obesity, and hyperlipidemia in patients with schizophrenia or schizoaffective disorder.MethodThis was a 6‐week, open label trial of ziprasidone 160 mg/day added to a stable dose of olanzapine or clozapine in 21 schizophrenia or schizoaffective patients with DM, impaired fasting glucose, or insulin resistance.ResultsTen olanzapine‐treated subjects and 11 clozapine‐treated subjects were enrolled in the study. There were no significant differences between the two groups at baseline for age, gender, education, ethnicity, BMI, cholesterol levels, or fasting glucose. At week 6, there were no significant changes in weight, BMI, cholesterol levels, or fasting glucose. There was no significant difference in psychotic, negative, or depressive symptoms. QTc significantly increased at week 2 but not at week 6.ConclusionsThe addition of 160 mg/day of ziprasidone was well tolerated but did not produce significant improvement in fasting glucose, insulin resistance, hyperlipidemia or lead to weight loss in olanzapine‐ or clozapine‐treated subjects with schizophrenia or schizoaffective disorder. Copyright © 2009 John Wiley & Sons, Ltd.
A study of soil water movement combining soil water potential with stable isotopes at two sites of shallow groundwater areas in the North China Plain
Authors: Song Xianfang , Wang Shiqin , Xiao Guoqiang , Wang Zhimin , Liu Xin , Wang Peng 
Published: March 2009

AbstractIn the shallow groundwater areas of the North China Plain (NCP), precipitation infiltration and evapotranspiration in the vertical direction are the main processes of the water cycle, in which the unsaturated zone plays an important role in the transformation process between precipitation and groundwater. In this paper, two typical sites in Cangzhou (CZ) and Hengshui (HS) of Hebei province with shallow water tables were selected to analyse the relationship among precipitation, soil water and groundwater. At each site, precipitation, soil water at depths 10, 20, 30, 50, 70, 100, 150, 200, 300 cm, and groundwater were sampled to analyse the stable isotope compositions of hydrogen and oxygen. The soil water potentials at the corresponding depths were observed. Although the climates at the two sites are similar, there are some differences in the infiltration process, soil water movement and groundwater recharge sources. Evaporation occurred at the upper depths, which led to the decrease of soil potential and the enrichment of heavy isotopes. At the CZ site, precipitation infiltrated with piston mode, and an obvious mixture effect existed during the infiltration process. Preferential flow may exist in the soil above 100 cm depth. However, at the HS site soil water moved in piston mode, and groundwater was mainly recharged by precipitation. When precipitation recharged the groundwater it experienced a strong evaporation effect. The results of the soil water movement mechanism provides the transformation relationship among precipitation, soil water and groundwater in the middle and eastern NCP. Copyright © 2009 John Wiley & Sons, Ltd.
A comparative Raman and CARS imaging study of colon tissue
Authors: Krafft Christoph , Ramoji Anuradha A. , Bielecki Christiane , Vogler Nadine , Meyer Tobias , Akimov Denis , Rösch Petra , Schmitt Michael , Dietzek Benjamin , Petersen Iver , Stallmach Andreas , Popp Jürgen 
Published: March 2009

AbstractAn experimental evaluation of the information content of two complimentary techniques, linear Raman and coherent anti‐Stokes Raman scattering (CARS) microscopy, is presented. CARS is a nonlinear variant of Raman spectroscopy that enables rapid acquisition of images within seconds in combination with laser scanning microscopes. CARS images were recorded from thin colon tissue sections at 2850, 1660, 1450 and 1000 cm–1 and compared with Raman images. Raman images were obtained from univariate and multivariate (k‐means clustering) methods, whereas all CARS images represent univariate results. Variances within tissue sections could be visualized in chemical maps of CARS and Raman images. However, identification of tissue types and characterization of variances between different tissue sections were only possible by analysis of cluster mean spectra, obtained from k‐means cluster analysis. This first comparison establishes the foundation for further development of the CARS technology to assess tissue. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Collagen scaffolds loaded with collagen‐binding NGF‐β accelerate ulcer healing
Authors: Sun Wenjie , Lin Hang , Chen Bing , Zhao Wenxue , Zhao Yannan , Xiao Zhifeng , Dai Jianwu 
Published: March 2009

AbstractStudies have shown that exogenous nerve growth factor (NGF) accelerates ulcer healing, but the inefficient growth factor delivery system limits its clinical application. In this report, we found that the native human NGF‐β fused with a collagen‐binding domain (CBD) could form a collagen‐based NGF targeting delivery system, and the CBD‐fused NGF‐β could bind to collagen membranes efficiently. Using the rabbit dermal ischemic ulcer model, we have found that this targeting delivery system maintains a higher concentration and stronger bioactivity of NGF‐β on the collagen membranes by promoting peripheral nerve growth. Furthermore, it enhances the rate of ulcer healing through accelerating the re‐epithelialization of dermal ulcer wounds and the formation of capillary lumens within the newly formed tissue area. Thus, collagen membranes loaded with collagen‐targeting human NGF‐β accelerate ulcer healing efficiently. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Effects of hydrostatic pressure on leporine meniscus cell‐seeded PLLA scaffolds
Authors: Gunja Najmuddin J. , Athanasiou Kyriacos A. 
Published: March 2009

AbstractHydrostatic pressure (HP) is an important component of the loading environment of the knee joint. Studies with articular chondrocytes and TMJ disc fibrochondrocytes have identified certain benefits of HP for tissue engineering purposes. However, similar studies with meniscus cells are lacking. Thus, in this experiment, the effects of applying 10 MPa of HP at three different frequencies (0, 0.1, and 1 Hz) to leporine meniscus cell‐seeded PLLA scaffolds were examined. HP was applied once every 3 days for 1 h for a period of 28 days. Constructs were analyzed for cellular, biochemical, and biomechanical properties. At t = 4 weeks, total collagen/scaffold was found to be significantly higher in the 10 MPa, 0 Hz group when compared with other groups. This despite the fact that the cell numbers/scaffold were found to be lower in all HP groups when compared with the culture control. Additionally, the total GAG/scaffold, instantaneous modulus, and relaxation modulus were significantly increased in the 10 MPa, 0 Hz group when compared with the culture control. In summary, this experiment provides evidence for the benefit of a 10 MPa, 0 Hz stimulus, on both biochemical and biomechanical aspects, for the purposes of meniscus tissue engineering using PLLA scaffolds. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010