bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adjuvant neuropeptides can improve neuropathic ligament healing in a rat model
Authors: Grorud Kelley W. , Jensen Kristina T. , Provenzano Paolo P. , Vanderby Ray 
Published: February 2007

AbstractDiminished healing in neuropathic tissues suggests an important regulatory role for peripheral neurogenic factors in connective tissue healing. Although neurogenic factors, including neuropeptides, can induce cell proliferation and influence inflammatory cell chemotaxis in vitro, there is little appreciation of the potential of neuropeptides to affect connective tissue healing in vivo. We created both efferent and afferent peripheral neuropathies in 55 female Wistar rats. First, we showed that neuropathy led to impaired healing of ruptured ligaments. We then showed that local delivery of specific neuropeptides could reverse the functional deficits of these neuropathic ligaments in only 2 weeks. In substance P and vasoactive intestinal peptide‐treated medial collateral ligaments (MCLs), the mechanical properties of these healing neuropathic tissues returned to values at or above normally innervated, intact ligaments. In addition, neuropeptide Y stimulated MCL healing in this model. These findings suggest a new paradigm to improve neuropathic soft connective tissue healing. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25: 703–712, 2007
Initial repair strengths of two methods for acute medial collateral ligament injuries of the elbow
Authors: Pichora James E. , Furukawa Keizo , Ferreira Louis M. , Faber Kenneth J. , Johnson James A. , King Graham J.W. 
Published: February 2007

AbstractThe purposes of this study were to compare the initial repair strength of the medial collateral ligament (MCL) of the elbow using trans‐osseous sutures and suture anchor methods and to determine the effect of repair pretensioning. Twelve, fresh‐frozen upper extremities (66 ± 5 years) were mounted in a valgus‐loading system. MCL repairs were performed using trans‐osseous suture and suture anchor methods with 20 N or 40 N pretensioning. A cyclic (0.5 Hz), valgus 40 N load was applied 12 cm distal to the elbow axis of flexion. The load was increased by 10 N every 200 cycles until a length increase of 5 mm or catastrophic failure of the repair occurred. Repairs pretensioned with 40 N endured a significantly higher number of cycles and failed at higher loads than those pretensioned with 20 N (p < 0.05). No difference was found in the cycles or load to failure between trans‐osseous sutures and suture anchors (p > 0.05). A higher magnitude of pretensioning of MCL repairs was found to increase initial repair strength suggesting that pretensioning should be performed clinically. Despite the comparable failure loads of the trans‐osseous suture and suture anchor methods, the failure mechanism differed between the two techniques. The suture anchors usually failed catastrophically when the sutures broke as they passed through the anchor eyelet, while the trans‐osseous sutures gradually elongated to the defined failure length by stretching and sliding through the ligament. The use of different suture anchors, suture sizes, or suture materials would likely influence the findings of this study and should be considered when applying these findings clinically. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:612–616, 2007
Distinct roles of bone morphogenetic proteins in osteogenic differentiation of mesenchymal stem cells
Authors: Luu Hue H. , Song Wen‐Xin , Luo Xiaoji , Manning David , Luo Jinyong , Deng Zhong‐Liang , Sharff Katie A. , Montag Anthony G. , Haydon Rex C. , He Tong‐Chuan 
Published: February 2007

AbstractEfficacious bone regeneration could revolutionize the clinical management of many bone and musculoskeletal disorders. Bone morphogenetic proteins (BMPs) can regulate the differentiation of mesenchymal stem cells into cartilage, bone, tendon/ligament, and fat lineages. Early data documented the osteogenic potential of rhBMP2 and rhBMP7/OP‐1. However, prior to this work that summarized several of our recent studies, no comprehensive analysis had been undertaken to characterize relative osteogenic activity of all BMPs. Using recombinant adenoviruses expressing 14 BMPs, we have demonstrated that, besides BMP2 and BMP7, BMP6 and BMP9 exhibit the highest osteogenic activity both in vitro and in vivo. We further demonstrated that several BMPs may exert synergistic effect on osteogenic differentiation, and that osteogenic BMPs produce a distinct set of molecular fingerprints during osteogenic differentiation. The reported work should expand our current understanding of BMP functions during osteogenic differentiation. It is conceivable that osteogenic BMPs (i.e., BMP2, 4, 6, 7, and 9) may be used to formulate synergistic pairs among themselves and/or with other less osteogenic BMPs for efficacious bone regeneration in clinical settings. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:665–677, 2007
In vivo medial and lateral tibial loads during dynamic and high flexion activities
Authors: Zhao Dong , Banks Scott A. , D'Lima Darryl D. , Colwell Clifford W. , Fregly Benjamin J. 
Published: February 2007

AbstractThough asymmetric loading between the medial and lateral compartments of total knee replacements may contribute to implant loosening and failure, the in vivo contact force distribution during dynamic daily activities remains unknown. This study reports in vivo medial and lateral contact forces experienced by a well‐aligned knee implant for a variety of activities. In vivo implant motion and total axial load data were collected from a single knee replacement patient performing treadmill gait (hands resting on handlebars), step up/down, lunge, and kneel activities. In vivo motion was measured using video fluoroscopy, while in vivo axial loads were collected simultaneously using an instrumented tibial component. An elastic foundation contact model employing linear and nonlinear polyethylene material properties was constructed to calculate medial and lateral contact forces based on the measured kinematics, total axial loads, and centers of pressure. For all activities, the predicted medial and lateral contact forces were insensitive to the selected material model. The percentage of medial to total contact force ranged from 18 to 60 for gait, 47 to 65 for step up/down, and 55 to 60 for kneel and lunge. At maximum load during the motion cycle, medial force was 1.2 BW for gait and 2.0 BW for step up/down, while the corresponding lateral forces were 1.0 and 1.5 BW, respectively. At mean load in the final static pose, medial force was 0.2 BW for kneel and 0.9 BW for lunge, with corresponding lateral forces of 0.1 and 0.7 BW, respectively. For this patient, a constant load split of 55% medial–45% lateral during loaded activity would be a reasonable approximation for these test conditions. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:593–602, 2007
From experimental research to an on‐farm tool for participatory monitoring and evaluation: an assessment of soil erosion risk in organic olive orchards
Authors: Milgroom J. , Gómez J. A. , Soriano M. A. , Fereres E. 
Published: February 2007

AbstractThe conservation of soil in agricultural systems prone to erosion can occur as a result of conscientious soil management practices by individual farmers. Although soil is one of the world's most important and highly threatened natural resources, and should be among the top priorities in organic agriculture, soil management practices and the effect of those practices on erosion risk are rarely, if ever, taken into account as a part of organic certification. In order to fulfil the need for a tool to monitor soil erosion in organic olive orchards in Southern Spain, we have developed a methodology that assesses erosion risk through an easy to use, illustrated erosion risk field manual to be used by farmers, inspectors for certifying agencies and field technicians. The methodology developed for the assessment is based on a simplified version of the Revised Universal Soil Loss Equation (RUSLE), combined with an evaluation of key visual symptoms of soil erosion. The methodology is described, and the results from use of the assessment in the field and in workshops with farmers are presented. The potential for use of the methodology for other crops and in other regions is discussed and also the future relevance of farmer‐oriented tools in the context of changing regulations within the common agricultural policy of the European Union. Copyright © 2007 John Wiley & Sons, Ltd.
Workplace isolation: Exploring the construct and its measurement
Authors: Marshall Greg W. , Michaels Charles E. , Mulki Jay P. 
Published: February 2007

AbstractVirtual offices are a growing trend in today's work environment and are expected to influence marketing roles dramatically, especially selling. These conditions may lead to perceptions of isolation, both socially and organizationally. Workplace isolation is a twodimensional construct that represents individuals' perceptions of isolation from others at work and includes perceived isolation from both colleagues and from the company's support network. This article reports the results of a four‐sample study to develop and validate a selfreport scale for measuring the two facets of workplace isolation. The scale's usefulness for future research and management applications are discussed. © 2007 Wiley Periodicals, Inc.
Determining the antecedents and consequences of donor‐perceived relationship quality—A dimensional qualitative research approach
Authors: Shabbir Haseeb , Palihawadana Dayananda , Thwaites Des 
Published: February 2007

AbstractIt is generally accepted that an understanding of quality conceptualizations is instrumental in the design and development of customer care programs. This study applies the dimensional qualitative research approach to develop a conceptual model, which demonstrates the antecedents and consequences of donor‐perceived relationship quality. This model shows that relationship benefits, service quality, trust, commitment, and satisfaction are the key antecedents of donor‐perceived relationship quality. Relationship benefits, service quality, and trust are also modeled as cognitive in nature, whereas commitment and satisfaction are modeled as affective variables. The model also highlights donor loyalty and positive word‐of‐mouth communications as the central consequences of donor‐perceived quality. The model gives rise to a useful research agenda for fund‐raising professionals. © 2007 Wiley Periodicals, Inc.
Nondigital tissue replantation and microsurgical spare part utilization in mutilated hands: Case reports and review
Authors: Nasir Serdar , Aydin Mustafa Asim , Gülgönen Ayan 
Published: February 2007

AbstractFive cases with microsurgical utilization of spare parts from unreplantable amputated segments are presented. Besides, nondigital composite tissue replantations are firstly reported in hand. They all resulted in satisfactory outcomes compared to alternative treatments without any donor site morbidity. © 2007 Wiley‐Liss, Inc. Microsurgery, 2007.
Perfusion, viability, and pedicle dependence in acute and delayed rat island skin flaps
Authors: Komorowska‐Timek Ewa , Timek Tomasz A. , Brady Sandra , Buncke Harry J. 
Published: February 2007

AbstractPurpose: Although surgical delay phenomenon has been widely investigated, its pathophysiology has not been fully elucidated. Methods: In 25 Spraque–Dawley rats, an 8 × 8 cm2 epigastric skin flap consisting of 4 vertical zones A through D (farthest from vascular pedicle) was outlined. All animals were perfused twice with colored fluorescent microspheres: immediately before and after flap elevation (Acute, n = 10) and before and after pedicle ligation on POD 8 (Delayed, n = 15). Results: After acute flap elevation, peripheral perfusion dropped significantly in zone C (0.29 ± 0.01 vs. 0.19 ± 0.04 ml g−1 min−1; P < 0.01) and zone D (0.33 ± 0.09 vs 0.01 ± 0.01 ml g−1 min−1; P < 0.01), while global flap perfusion remained unchanged. Total and regional blood flow did not change in the Delayed group after pedicle ligation. Conclusions: Elevation of a pedicled flap caused significant decrease in distal flap perfusion while maintaining proximal and total flap perfusion. Eight‐day delay was adequate to establish sufficient flap perfusion independent of the vascular pedicle. © 2007 Wiley‐Liss, Inc. Microsurgery, 2007.
Intramolecular hydrogen bonding of novel o‐hydroxythioacetophenones and related compounds evaluated by deuterium isotope effects on 13C chemical shifts
Authors: Nguyen Trung Thanh , Le Thach Ngoc , Duus Fritz , Hansen Bjarke K. V. , Hansen Poul Erik 
Published: February 2007

AbstractA new class of compounds, the 2‐hydroxythioacetophenones, and related compounds have recently been synthesized. The hydrogen‐bond system has been characterized by NMR chemical shifts and deuterium isotope effects on these as well as by DFT calculations. Use of solid‐state 13C NMR has enabled measurements of the intrinsic deuterium isotope effects of the most abundant tautomer of β‐thioxoketones. The compounds show very interesting long‐range deuterium isotope effects on the thiocarbonyl carbon. The intramolecular hydrogen bonds of o‐hydroxythioacetophenones are found to be slightly stronger than those of the corresponding acetophenones. The reactivity and stability of the compounds can be related to hydrogen bonding and to the presence of electron donating substituents. Copyright © 2007 John Wiley & Sons, Ltd.
Structure elucidation and complete NMR spectral assignments of a novel sesquiterpene glycoside from Ixeris sonchifolia
Authors: Ye Guan , Fang Jing‐jing , Fan Ming‐song , Huang Cheng‐gang 
Published: February 2007

AbstractA novel sesquiterpene glycoside was isolated from the whole plant of Ixeris sonchifolia. The structure was established as 1(10)E‐4Z‐3α‐hydroxy‐germacra‐1(10),4,11(13)‐trien‐6, 12‐olide‐14‐O‐β‐D‐glucopyranoside (1) on the basis of spectroscopic techniques and chemical analysis. Copyright © 2007 John Wiley & Sons, Ltd.
G2/M checkpoint gene expression in developing germ cells
Authors: Hasthorpe Suzanne , Tainton Kellie , Peart Melissa , Roeszler Kelly N. , Bell Katrina M. , Lusby Patricia E. , Hutson John M. , Tymms Martin J. 
Published: February 2007

AbstractCell cycle progression is prevented by signal transduction pathways known as checkpoints which are activated in response to replication interference and DNA damage. We cloned a G2/M cell cycle phase‐related checkpoint gene from a neonatal mouse testis cDNA library which was identified as mouse claspin, a proposed adaptor protein for Chk1. As part of a study on germ cell differentiation we examined the expression of the checkpoint gene, Chk1, and claspin at 12.5 and 14.5 days post coitum (dpc) and in the post‐natal phase. Chk1 mRNA expression increased from 12.5 to 14.5 dpc in female gonads and was strong in males at both time points. Claspin however, was not detected until 14.5 dpc. This suggests there may be some dissociation of claspin expression from Chk1 in fetal germ cell development. Chk1 and claspin expression was also studied in testis over the first 3 days following birth, when apoptosis regulates germ stem cell number. We modulated checkpoint‐related gene expression in testis using the anti‐metabolite, 5‐fluorouracil, resulting in increased apoptosis and upregulation of Chk1 (P < 0.0001) and Cdc2 (P < 0.02) mRNA. Although we do not fully understand the role checkpoint gene expression has during mammalian germ cell development this report is the first to show the expression of checkpoint‐related genes in early mammalian germ cells. Mol. Reprod. Dev. 74: 531–538, 2007. © 2007 Wiley‐Liss, Inc.
Differential expression and localization of four connexins in the ovary of the ayu (Plecoglossus Altivelis)
Authors: Yamamoto Yoji , Yoshizaki Goro , Takeuchi Toshio , Soyano Kiyoshi , Itoh Fuminari , Patiño Reynaldo 
Published: February 2007

AbstractThe post‐vitellogenic oocytes of teleost fish are generally unresponsive to maturation‐inducing hormone (MIH) until a luteinizing hormone (LH) surge stimulates sensitivity via the acquisition of oocyte‐maturational competence (OMC). Heterologous gap junctions (GJs) between granulosa cells and the oocyte have been previously implicated in the regulation of oocyte maturation in various vertebrate species. Although heterologous GJ are present in ovarian follicles of ayu (Plecoglossus altivelis), their role in maturation remains unclear. In the present study, we cloned and characterized complementary DNAs for GJ protein connexin (Cx), and examined the expression pattern of Cx messenger RNAs in the ayu ovary. Four Cx cDNAs with predicted molecular masses of 32.1 (Cx32.1), 34.9 (Cx34.9), 44.1 (Cx44.1), and 44.2 (Cx44.2) kDa, respectively, were cloned. Northern blot analysis revealed that the levels of Cx44.1 and Cx44.2 transcripts were similar during the vitellogenic and ovulatory stages. Cx32.1 transcripts were more abundant during the vitellogenic stage, but their levels declined thereafter. Cx34.9 transcript levels increased during the vitellogenic stage and remained high during the acquisition of OMC. In situ hybridization revealed that Cx44.1 and Cx44.2 signals were restricted to the oocyte, whereas the Cx32.1 and Cx34.9 signals were detected in both cellular fractions. Furthermore, a dye‐transfer assay revealed the presence of functional GJs between the oocytes and follicle cells. These results suggest that Cx34.9 contributes to the formation of heterologous GJs between oocytes and granulosa cells. Moreover, GJs formed by Cx34.9 may function during the LH‐dependent acquisition of OMC and the MIH‐dependent resumption of meiosis in ayu. Mol. Reprod. Dev. 74: 1113–1123, 2007. © 2007 Wiley‐Liss, Inc.
Vitrification devices affect structural and molecular status of in vitro matured ovine oocytes
Authors: Succu S. , Leoni G.G. , Bebbere D. , Berlinguer F. , Mossa F. , Bogliolo L. , Madeddu M. , Ledda S. , Naitana S. 
Published: February 2007

AbstractWe evaluated the effect of three different cryodevices on membrane integrity, tubulin polymerization, maturation promoting factor (MPF) activity and developmental competence of in vitro matured (IVM) ovine oocytes. IVM oocytes were exposed during 3 min to 7.5% DMSO and 7.5% ethylene glycol (EG) in TCM199 and 25 sec to 0.5 M sucrose, 16.5% DMSO and 16.5% EG, loaded in open pulled straws (OPS), cryoloops (CL) or cryotops (CT) and immersed into liquid nitrogen. Untreated (CTR) or exposed to vitrification solutions but not cryopreserved (EXP) oocytes were used as controls. After warming, double fluorescent staining evidenced a lower membrane integrity in vitrified groups compared to the controls (P < 0.01). After in vitro fertilization and culture OPS and CL groups evidenced a lower cleavage rate than CT and controls (P < 0.01) while blastocysts were obtained only in CL and EXP, at a lower rate than CTR (P < 0.01). All vitrified groups showed alterations in spindle conformation, which were partially recovered in OPS and CT groups. MPF activity was lower in treated compared to CTR and CT showed the lowest value (P < 0.01). After 2 hr culture MPF activity was restored in all groups except CT. Parthenogenetic activation was higher in treated compared to CTR and CT evidenced the highest value. Our results indicate that cryodevice influences not only the ability to survive cryopreservation but is also associated with molecular alterations which affect developmental competence. Mol. Reprod. Dev. 74: 1337–1344, 2007. © 2007 Wiley‐Liss, Inc.
Dynamics of DNA‐demethylation in early mouse and rat embryos developed in vivo and in vitro
Authors: Zaitseva Ioulia , Zaitsev Sergei , Alenina Natalia , Bader Michael , Krivokharchenko Alexander 
Published: February 2007

AbstractVirtually all mammalian species including mouse, rat, pig, cow, and human, but not sheep and rabbit, undergo genome‐wide epigenetic reprogramming by demethylation of the male pronucleus in early preimplantation development. In this study, we have investigated and compared the dynamics of DNA demethylation in preimplantation mouse and rat embryos by immunofluorescence staining with an antibody against 5‐methylcytosine. We performed for the first time a detailed analysis of demethylation kinetics of early rat preimplantation embryos and have shown that active demethylation of the male pronucleus in rat zygotes proceeds with a slower kinetic than that in mouse embryos. Using dated mating we found that equally methylated male and female pronuclei were observed at 3 hr after copulation for mouse and 6 hr for rat embryos. However, a difference in methylation levels between male and female pronuclei could be observed already at 8 hr after copulation in mouse and 10 hr in rat. At 10 hr after copulation, mouse male pronuclei were completely demethylated, whereas rat zygotes at 16 hr after copulation still exhibited detectable methylation of the male pronucleus. In addition in both species, a higher DNA methylation level was found in embryos developed in vitro compared to in vivo, which may be one of the possible reasons for the described aberrations in embryonic gene expression after in vitro embryo manipulation and culture. Mol. Reprod. Dev. 74: 1255–1261, 2007. © 2007 Wiley‐Liss, Inc.
Possible involvement of CD81 in acrosome reaction of sperm in mice
Authors: Tanigawa Maya , Miyamoto Kiyoko , Kobayashi Satoshi , Sato Masahiro , Akutsu Hidenori , Okabe Masaru , Mekada Eisuke , Sakakibara Keiichi , Miyado Mami , Umezawa Akihiro , Miyado Kenji 
Published: February 2007

AbstractTetraspanin CD81 is closely homologous in amino acid sequence with CD9. CD9 is well known to be involved in sperm–egg fusion, and CD81 has also been reported to be involved in membrane fusion events. However, the function of CD81 as well as that of CD9 in membrane fusion remains unclear. Here, we report that disruption of the mouse CD81 gene led to a reduction in the fecundity of female mice, and CD81−/− eggs had impaired ability to fuse with sperm. Furthermore, we demonstrated that when CD81−/− eggs were incubated with sperm, some of the sperm that penetrated into the perivitelline space of CD81−/− eggs had not yet undergone the acrosome reaction, indicating that the impaired fusibility of CD81−/− eggs may be in part caused by failure of the acrosome reaction of sperm. In addition, we showed that CD81 was highly expressed in granulosa cells, somatic cells that surround oocytes. Our observations suggest that there is an interaction between sperm and CD81 on somatic cells surrounding eggs before the direct interaction of sperm and eggs. Our results may provide new clues for clarifying the cellular mechanism of the acrosome reaction, which is required for sperm–egg fusion. Mol. Reprod. Dev. 75: 150–155, 2008. © 2007 Wiley‐Liss, Inc.