bluuebunny/crossref_metadata_2025_split huggingface dataset:
Former foodstuffs in feed: a minireview of recent findings
Authors: Srikanthithasan Karthika , Giorgino Andrea , Fiorilla Edoardo , Ozella Laura , Gariglio Marta , Schiavone Achille , Marín Andrés Luis Martínez , Diaz Vicuna Elena , Forte Claudio 
Published: March 2024

AbstractThe sustainability of all productive activities, including livestock farming, becomes a fundamental challenge in the current scenario. Livestock production faces both old and new challenges related to climate change, food safety, and feed-food competition. The latter aspect has recently become a hot topic, and many researchers are turning their attention to this issue. According to circular economy principles, former foodstuffs have characteristics that make them a promising source of raw material for animal feed. The main objective of the present review is to provide a brief overview of the most recent studies (published between 2016 and 2022) addressing the dietary inclusion of former foodstuffs for livestock. The articles analyzed cover key findings from both in vitro and in vivo studies of former foodstuffs included in the diets for pigs, cows, and broilers, and assess the associated safety aspects. The articles provide information on livestock performances and product quality, as well as feed digestibility, fecal microbiota, and blood analysis. Although the evidence supports the inclusion of former foodstuffs in livestock diets as a safe, effective, and sustainable ingredient, this analysis of the most recent literature also highlights gaps in our knowledge that need to be filled. The present overview will help researchers plan future research and standardize and promote the inclusion of former food products in livestock diets.
Associations of plasma proteomics and age-related outcomes with brain age in a diverse cohort
Authors: Casanova Ramon , Walker Keenan A. , Justice Jamie N. , Anderson Andrea , Duggan Michael R. , Cordon Jenifer , Barnard Ryan T. , Lu Lingyi , Hsu Fang-Chi , Sedaghat Sanaz , Prizment Anna , Kritchevsky Stephen B. , Wagenknecht Lynne E. , Hughes Timothy M. 
Published: March 2024

AbstractMachine learning models are increasingly being used to estimate “brain age” from neuroimaging data. The gap between chronological age and the estimated brain age gap (BAG) is potentially a measure of accelerated and resilient brain aging. Brain age calculated in this fashion has been shown to be associated with mortality, measures of physical function, health, and disease. Here, we estimate the BAG using a voxel-based elastic net regression approach, and then, we investigate its associations with mortality, cognitive status, and measures of health and disease in participants from Atherosclerosis Risk in Communities (ARIC) study who had a brain MRI at visit 5 of the study. Finally, we used the SOMAscan assay containing 4877 proteins to examine the proteomic associations with the MRI-defined BAG. Among N = 1849 participants (age, 76.4 (SD 5.6)), we found that increased values of BAG were strongly associated with increased mortality and increased severity of the cognitive status. Strong associations with mortality persisted when the analyses were performed in cognitively normal participants. In addition, it was strongly associated with BMI, diabetes, measures of physical function, hypertension, prevalent heart disease, and stroke. Finally, we found 33 proteins associated with BAG after a correction for multiple comparisons. The top proteins with positive associations to brain age were growth/differentiation factor 15 (GDF-15), Sushi, von Willebrand factor type A, EGF, and pentraxin domain-containing protein 1 (SEVP 1), matrilysin (MMP7), ADAMTS-like protein 2 (ADAMTS), and heat shock 70 kDa protein 1B (HSPA1B) while EGF-receptor (EGFR), mast/stem-cell-growth-factor-receptor (KIT), coagulation-factor-VII, and cGMP-dependent-protein-kinase-1 (PRKG1) were negatively associated to brain age. Several of these proteins were previously associated with dementia in ARIC. These results suggest that circulating proteins implicated in biological aging, cellular senescence, angiogenesis, and coagulation are associated with a neuroimaging measure of brain aging.
Kerosene condenses in the trachea following inhalation
Authors: Takei Sella , Kinoshita Hiroshi , Kawahara Sachiko , Kumihashi Mitsuru , Jamal Mostofa , Yamashita Tadayoshi , Tanaka Etsuko , Abe Hiroko , Tsutsui Kunihiko , Kimura Shoji 
Published: March 2024

Abstract
Purpose
We have investigated the absorption dynamics of petroleum fuel components from the analytical results of autopsy samples.

Methods
Post-mortem samples of the severely burned case, including femoral blood, intratracheal contents (mucus) and intratracheal gas-phase samples were collected, and analysed by gas chromatography-mass spectrometer with head-space solid-phase microextraction.

Results
The composition of flammable substances in the tracheal gas phase differed slightly from that in mucus.

Conclusion
High-boiling point components are retained in the trachea, whereas relatively lower-boiling point components are detected predominantly in the tracheal gas phase and blood.
Efficacy and Safety of Bariatric Surgery in Dutch People Living with HIV: a Retrospective Matched Cohort Analysis
Authors: Zino Leena , Qing Chen Rou , Deden Laura , Hazebroek Eric , Richel Olivier , Colbers Angela , Burger David M ,   On behalf of the DATO Research Group
Published: March 2024

Abstract
Purpose
Obesity is rising among people with HIV (PLWH), sparking interest in bariatric surgery (BS) for this group. Yet, large-scale comparative research on BS outcomes in PLWH is lacking.

Methods
We performed a retrospective, matched cohort analysis in PLWH and HIV uninfected controls. Subjects were retrieved from the Dutch Audit for Treatment of Obesity (DATO) registry. Matching (1:7 ratio) included age (± 5-years), sex, body-mass index (BMI) of ± 3 kg/m2, surgery type, and associated health problems (AHPs) at baseline. The primary endpoint was total weight loss percentage (%TWL) ≥ 20% achieved at 1-year post-BS. Secondary endpoints were cumulative %TWL achieved at 2-years post-BS, a reported remission or improvement in AHPs post-BS, and surgical complications, both at 1-year post-BS. Comparisons were performed using conditional logistic regression.

Results
Twenty-seven PLWH and 168 controls were included. At 1-year post-BS, 89% PLWH achieved ≥ 20%TWL, compared to 94% of controls (p = 0.4). Cumulative %TWL at 2-years post-BS were 82% and 92% in PLWH and controls, respectively (p = 0.2). Improvement rates in hypertension and type 2 diabetes mellitus were 50% and 86% in PLWH, versus 87% and 87% in controls. Full remission occurred in 20% and 71% of PLHIV, versus 49% and 44% of controls, respectively. No improvement or remission was observed for dyslipidaemia in PLHIV compared to 54% improvement and 29% remission in controls. Surgical complications were 0% in PLHIV and 13% (n = 21) in controls.

Conclusion
Efficacy and safety outcomes of BS were similar between PLWH and controls except for the lack of improvement in dyslipidaemia in PLWH.

Graphical abstract
Subventionen für „kleine Jobs“:
Authors: Riphahn Regina T. 
Published: March 2024

ZusammenfassungDie Grohmann-Vorlesung des Jahres 2023 beschäftigt sich mit dem Phänomen der „kleinen Jobs“ in Deutschland. Zunächst wird der institutionelle und historische Hintergrund von Minijobs erläutert und die Intensität ihrer Nutzung beschrieben. Anschließend fasst der Text die Inhalte von drei empirischen Studien zusammen. Diese setzen sich mit der Frage auseinander ob (i) Arbeitgeber reguläre Beschäftigung durch Minijobs ersetzen, (ii) Minijobs zur „motherhood penalty“ in Deutschland beitragen und (iii) ob Midijobs Übergänge aus Minijobs in reguläre sozialversicherungspflichtige Beschäftigung erleichtert haben. Die Vorlesung schließt mit einer Betrachtung möglicher Regelungsalternativen für „kleine Jobs“ in Deutschland.
Comparative Effects of Including Inorganic, Organic, and Hydroxy Zinc Sources on Growth Development, Egg Quality, Mineral Excretion, and Bone Health of Laying Quails
Authors: Olgun Osman , Gül Esra Tuğçe , Kılınç Gözde , Gökmen Fatih , Yıldız Alpönder , Uygur Veli , Sarmiento-García Ainhoa 
Published: March 2024

AbstractThe purpose of this study was to determine the effects of the dietary addition of zinc (Zn) in the form of sulphate (Zn-S), glycine (Zn-G), and hydroxychloride (Zn-H) on quail performance, eggshell quality, antioxidant status, mineral excretion, biomechanical properties and mineralization of bone. A total of 75 female quails (10-weeks-old) were randomly distributed into 3 groups with 5 replications, each with 5 female quails. Treatment groups were set up by adding Zn-S and Zn-H as the inorganic form and Zn-G as the organic form of zinc to the corn-soybean basal diet (34.14 mg/kg Zn) to obtain 50 mg/kg Zn and the feeding experiment lasted for 12 weeks. Performance parameters and egg production were not impaired by the Zn source (P > 0.05). The inclusion of Zn-S in the diet produced a reduction in eggshell thickness, while an improvement in yolk antioxidant capacity (measured as MDA concentration) was detected compared to the other Zn sources (P < 0.05). Shear strength and cortical bone area increased (P < 0.05) with Zn-G supplementation, however, the mineral concentration of the tibia was higher (P < 0.01) in those quails who had received Zn-H. Lower levels of mineral excretion were observed in both types of supplementations compared to Zn-S. Therefore, it can be stated that Zn-G or Zn-H supplementation in the diet of laying quails could be an interesting strategy to reduce mineral excretion and improve bone mineralization without affecting performance compared to Zn-S. However, further studies are needed to determine the differences between Zn-G and Zn-H.
Glioblastoma Cells Use an Integrin- and CD44-Mediated Motor-Clutch Mode of Migration in Brain Tissue
Authors: Anderson Sarah M. , Kelly Marcus , Odde David J. 
Published: March 2024

Abstract
Purpose
Glioblastoma (GBM) is an aggressive malignant brain tumor with 2 year survival rates of 6.7% (Stupp et al. in J Clin Oncol Off J Am Soc Clin Oncol 25:4127–4136, 2007; Mohammed et al. in Rep Pract Oncol Radiother 27:1026–1036, 2002). One key characteristic of the disease is the ability of glioblastoma cells to migrate rapidly and spread throughout healthy brain tissue (Lefranc et al. in J Clin Oncol Off J Am Soc Clin Oncol 23:2411–2422, 2005; Hoelzinger et al. in J Natl Cancer Inst 21:1583–1593, 2007). To develop treatments that effectively target cell migration, it is important to understand the fundamental mechanism driving cell migration in brain tissue. Several models of cell migration have been proposed, including the motor-clutch, bleb-based motility, and osmotic engine models.

Methods
Here we utilized confocal imaging to measure traction dynamics and migration speeds of glioblastoma cells in mouse organotypic brain slices to identify the mode of cell migration.

Results
We found that nearly all cell-vasculature interactions reflected pulling, rather than pushing, on vasculature at the cell leading edge, a finding consistent with a motor-clutch mode of migration, and inconsistent with an osmotic engine model or confined bleb-based migration. Reducing myosin motor activity, a key component in the motor-clutch model, was found to decrease migration speed at high doses for all cell types including U251 and 6 low-passage patient-derived xenograft lines (3 proneural and 3 mesenchymal subtypes). Variable responses were found at low doses, consistent with a motor-clutch mode of migration which predicts a biphasic relationship between migration speed and motor-to-clutch ratio. Targeting of molecular clutches including integrins and CD44 slowed migration of U251 cells.

Conclusions
Overall we find that glioblastoma cell migration is most consistent with a motor-clutch mechanism to migrate through brain tissue ex vivo, and that both integrins and CD44, as well as myosin motors, play an important role in constituting the adhesive clutch.
Silica Nanoparticles Decrease Glutamate Uptake in Blood–Brain Barrier Components
Authors: Sánchez-Cano Fredy , Hernández-Kelly Luisa C. , Ortega Arturo 
Published: March 2024

AbstractGlutamate is the major excitatory amino acid in the vertebrate brain, playing an important role in most brain functions. It exerts its activity through plasma membrane receptors and transporters, expressed both in neurons and glia cells. Overstimulation of neuronal glutamate receptors is linked to cell death in a process known as excitotoxicity, that is prevented by the efficient removal of the neurotransmitter through glutamate transporters enriched in the glia plasma membrane and in the components of the blood–brain barrier (BBB). Silica nanoparticles (SiO2-NPs) have been widely used in biomedical applications and directed to enter the circulatory system; however, little is known about the potential adverse effects of SiO2-NPs exposure on the BBB transport systems that support the critical isolation function between the central nervous system (CNS) and the peripheral circulation. In this contribution, we investigated the plausible SiO2-NPs-mediated disruption of the glutamate transport system expressed by BBB cell components. First, we evaluated the cytotoxic effect of SiO2-NPs on human brain endothelial (HBEC) and Uppsala 87 Malignant glioma (U-87MG) cell lines. Transport kinetics were evaluated, and the exposure effect of SiO2-NPs on glutamate transport activity was determined in both cell lines. Exposure of the cells to different SiO2-NP concentrations (0.4, 4.8, 10, and 20 µg/ml) and time periods (3 and 6 h) did not affect cell viability. We found that the radio-labeled D-aspartate ([3H]-D-Asp) uptake is mostly sodium-dependent, and downregulated by its own substrate (glutamate). Furthermore, SiO2-NPs exposure on endothelial and astrocytes decreases [3H]-D-Asp uptake in a dose-dependent manner. Interestingly, a decrease in the transporter catalytic efficiency, probably linked to a diminution in the affinity of the transporter, was detected upon SiO2-NPs. These results favor the notion that exposure to SiO2-NPs could disrupt BBB function and by these means shed some light into our understanding of the deleterious effects of air pollution on the CNS.
Pelvic Pain and Sexual Function in Primiparous Pregnant Women with and without High/Severe Fear of Childbirth
Authors: Çankaya Seyhan , Bay Habibe , Akın Bihter 
Published: March 2024

Abstract
Introduction
This study aimed to determine pelvic pain and sexual dysfunction in primiparous pregnant women with varying levels of fear of childbirth (FOC).

Methods
This is a descriptive correlational study. This study was conducted with 400 pregnant women at 24–40 weeks of gestation who applied to the obstetrics polyclinic of a hospital between January 2022 and March 2022. Data were collected through a personal information form, the Wijma Delivery Expectancy Questionnaire (W-DEQ-A), the Pelvic Pain Impact Questionnaire (PPIQ), and Golombok-Rust Inventory of Sexual Satisfaction (GRISS) Female Form.

Results
Of the 400 pregnant women, 232 (58%) had high/severe FOC. High/severe FOC was a risk factor that negatively affected pelvic pain and sexual function (frequency of sexual intercourse, communication, sexual satisfaction, avoidance, touching, vaginismus, and anorgasmia) in pregnant women (p < 0.000). In addition, pregnant women with high/severe FOC avoided vaginal examination (p = 0.016), had higher fear of vaginal examination (p = 0.004), experienced more pain or burning during sexual intercourse (p = 0.017), and had more fear of pain during sexual intercourse (p = 0.003).

Conclusions
High/severe FOC during pregnancy is an important risk factor that negatively affects pelvic pain and sexual function.

Policy Implications
Identifying pregnant women with high/severe FOC and support programs such as antenatal education programs or cognitive coping strategies to cope with FOC may be useful in preventing pelvic pain and sexual functions that may be negatively affected.
Vectorgastrogram: dynamic trajectory and recurrence quantification analysis to assess slow wave vector movement in healthy subjects
Authors: Prats-Boluda Gema , Martinez-de-Juan Jose L. , Nieto-del-Amor Felix , Termenon María , Varón Cristina , Ye-Lin Yiyao 
Published: March 2024

AbstractFunctional gastric disorders entail chronic or recurrent symptoms, high prevalence and a significant financial burden. These disorders do not always involve structural abnormalities and since they cannot be diagnosed by routine procedures, electrogastrography (EGG) has been proposed as a diagnostic alternative. However, the method still has not been transferred to clinical practice due to the difficulty of identifying gastric activity because of the low-frequency interference caused by skin–electrode contact potential in obtaining spatiotemporal information by simple procedures. This work attempted to robustly identify the gastric slow wave (SW) main components by applying multivariate variational mode decomposition (MVMD) to the multichannel EGG. Another aim was to obtain the 2D SW vectorgastrogram VGGSW from 4 electrodes perpendicularly arranged in a T-shape and analyse its dynamic trajectory and recurrence quantification (RQA) to assess slow wave vector movement in healthy subjects. The results revealed that MVMD can reliably identify the gastric SW, with detection rates over 91% in fasting postprandial subjects and a frequency instability of less than 5.3%, statistically increasing its amplitude and frequency after ingestion. The VGGSW dynamic trajectory showed a statistically higher predominance of vertical displacement after ingestion. RQA metrics (recurrence ratio, average length, entropy, and trapping time) showed a postprandial statistical increase, suggesting that gastric SW became more intense and coordinated with a less complex VGGSW and higher periodicity. The results support the VGGSW as a simple technique that can provide relevant information on the “global” spatial pattern of gastric slow wave propagation that could help diagnose gastric pathologies.
Structural complexity predicts consensus readability in online discussions
Authors: Hadfi Rafik , Ito Takayuki 
Published: March 2024

Abstract
The intricate relationship between structure and function spans various disciplines, from biology to management, offering insights into predicting interesting features of complex systems. This interplay is evident in online forums, where the organization of the threads interacts with the message’s meaning. Assessing readability in these discussions is vital for ensuring information comprehension among diverse audiences. This assessment is challenging due to the complexity of natural language compounded by the social and temporal dynamics within social networks. One practical approach involves aggregating multiple readability metrics as a consensus alignment. In this study, we explore whether the structural complexity of online discussions can predict consensus readability without delving into the semantics of the messages. We propose a consensus readability metric derived from well-known readability tests and a complexity metric applied to the tree structures of Reddit discussions. Our findings indicate that this proposed metric effectively predicts consensus readability based on the complexity of discourse structure.
Liraglutide prevents cellular senescence in human retinal endothelial cells (HRECs) mediated by SIRT1: an implication in diabetes retinopathy
Authors: Hou Lihua , Du Jianying , Dong Yongxiao , Wang Min , Wang Libo , Zhao Jifei 
Published: March 2024

AbstractDiabetes mellitus (DM) is a chronic metabolic disorder affecting millions of people worldwide, characterized by dysregulated glucose homeostasis and hyperglycemia. Diabetic retinopathy (DR) is one of the serious multisystemic complications. Aging is an important risk factor for DR. Endothelial sirtuin 1 (SIRT1) plays an important role in regulating the pathophysiology of glucose metabolism, cellular senescence, and aging. Liraglutide, an analog of Glucagon-like peptide 1 (GLP-1), has been widely used in the treatment of DM. However, the effects of Liraglutide on DR are less reported. Here, we investigated whether treatment with Liraglutide has beneficial effects on high glucose (HG)-induced injury in human retinal microvascular endothelial cells (HRECs). First, we found that exposure to HG reduced the expression of glucagon-like peptide 1 receptor 1 (GLP-1R). Additionally, Liraglutide ameliorated HG-induced increase in the expression of vascular endothelial growth factor-A (VEGF-A) and interleukin 6 (IL-6). Importantly, Liraglutide ameliorated cellular senescence and increased telomerase activity in HG-challenged HRECs. Liraglutide also reduced the levels of p53 and p21. Mechanistically, Liraglutide restored the expression of SIRT1 against HG. In contrast, the knockdown of SIRT1 abolished the protective effects of Liraglutide in cellular senescence of HRECs. Our findings suggest that Liraglutide might possess a benefit on DR mediated by SIRT1.
Scientific note on a Neotropical wasp preying on stingless bees
Authors: Boff Samuel , Somavilla Alexandre 
Published: March 2024

AbstractThe antagonistic interaction between wasps and bees has long driven the attention of beekeepers and evolutionary entomologists. In the most classic reported interaction, Vespa velutina may invade the nest of honeybees and kill many of workers. Although this interaction has been registered to Asian honeybees, which was shown how to defend the nests, little or no attention has been given to similar interactions in the Neotropical region. Here, we report for the first time an antagonistic interaction between the wasp Polybia ignobilis and the stingless bees, Tetragonisca angustula. The wasp of this species was recorded killing and chewing guard bees near at the nest entrance of the stingless bee. Although our report is limited to answer evolutionary aspects of this interaction, it raises questions which may help to understand in the future the reasons this behavior evolved.