bluuebunny/crossref_metadata_2025_split huggingface dataset:
Going deep into Parazoanthus axinellae (Anthozoa: Zoantharia) complex: description of two species in the Alboran Sea based on an integrative approach
Authors: Rosales Ruiz Alfredo , Ocaña Oscar , de la Herrán Roberto , Navajas-Pérez Rafael , Ruiz Rejón Carmelo , Congil Ross Ander , Robles Francisca 
Published: February 2025

Abstract

Parazoanthus axinellae Schmidt, 1862 (Anthozoa: Zoantharia) has been historically divided into different taxa at various levels (varieties, morphotypes, or subspecies) and is considered a species complex by some authors. This species has a wide distribution, is a key part of coralligenous habitats, and constitutes one of the main ecosystems in the Alboran and Mediterranean Seas. In this work, we propose the reclassification of one subspecies and a new species of Parazoanthus: Parazoanthus brevitentacularis stat. nov. and Parazoanthus franciscae sp. nov. The first was described as a morphotype (named “stocky”) and as the subspecies P. axinellae brevitentacularis, while the second is described here for the first time. An integrative approach, combining morphological, ecological, histological, and genetic analyses, allowed us to detect enough variability to establish this new species and led us to better understand the diversity of this group. Morphological and ecological analyses have been performed in situ by observing the main different features of the species. Histological examinations to propose the systematics of the species and the main diagnostic characters to identify them were performed based on the macro-anatomy, micro-anatomy, and the features of the cnidome. Genetic analyses were performed using common molecular markers (COI and ITS) and mitochondrial genome sequencing (MGS). The COI region was limited in establishing informative relationships within the species. MGS was a powerful tool to assess diversity, although somewhat limited due to the small number of genome sequences available, and the slow evolution of mitochondrial genomes in Anthozoa. Ribosomal ITS showed wider distances between taxa, resulting in the phylogenetic trees being most congruent with the ecological, morphological, and histological analyses.
Mitochondrial genome of Amphiglena cf. mediterranea IV, a small and cryptic polychaete (Annelida: Sabellidae)
Authors: Putignano Matteo , Tilic Ekin 
Published: February 2025

Abstract
The genus Amphiglena, comprising minute sabellid worms, is known for its cryptic diversity in the Mediterranean region. This study presents the first complete mitochondrial genome for the genus, corresponding to Amphiglena cf. mediterranea “clade IV” sensu Tilic et al. (2019). The mitogenome features 13 protein-coding genes, two rRNAs, and 22 tRNAs, totalling 16,401 base pairs. Notably, a novel gene order was identified, distinct from previously reported mitochondrial gene arrangement in annelids. This work provides essential genetic data for investigating mitochondrial genome evolution within Sabellidae and for understanding the evolutionary relationships within Amphiglena.
Single-molecule magnetic tweezers to unravel protein folding dynamics under force
Authors: Tapia-Rojo Rafael 
Published: February 2025

Abstract
Single-molecule magnetic tweezers have recently emerged as a powerful technique for measuring the equilibrium dynamics of individual proteins under force. In magnetic tweezers, a single protein is tethered between a glass coverslip and a superparamagnetic bead, and by applying and controlling a magnetic field, the protein is mechanically stretched while force-induced conformational changes are measured by tracking the vertical position of the bead. The soft trap created by the magnetic field provides intrinsic force-clamp conditions, which makes magnetic tweezers particularly well-suited to measure protein conformational dynamics. Traditionally employed to study DNA due to their initially low spatial and temporal resolutions, magnetic tweezers instrumentation has experienced significant progress in recent years. The development of high-speed cameras, stronger illumination sources, advanced image analysis algorithms, and dedicated chemical functionalization strategies, now allow for high-resolution and ultra-stable experiments. Together with their ability to apply and control low forces, magnetic tweezers can capture long-term equilibrium protein folding dynamics, not possible with any other technique. These capabilities have proven particularly valuable in the study of force-sensing protein systems, which often exhibit low mechanical stabilities that are challenging to measure with other techniques. In this review, we will discuss the current status of magnetic tweezers instrumentation for studying protein folding dynamics, focusing on both the instrumental aspects and methodologies to interpret nanomechanical experiments.
Who Wants to be Mindful? Personality Predictors of Meditation Practice
Authors: Ryakhovskaya Yana , Van Dam Nicholas T. , Smillie Luke D. 
Published: February 2025

Abstract

Objectives
Meditation has received increasing interest from scientists and the public for potential benefits to mental health and well-being. We examined personality predictors and expectancies of meditation to understand who engages with meditation. We also investigated whether personality traits predict interest in information relating to meditation and well-being activities, and self-selection into meditation research.

Method
Across two studies (n = 377 and n = 667), participants completed a range of personality and meditation-related questionnaires. To examine selection effects, two versions of the study advertisement were used: The first described the study as focused on meditation whereas the second omitted this wording. Data were analysed using Pearson’s correlations, logistic and linear regression models.

Results
We found that increased openness and agreeableness were associated with increased likelihood of having tried meditation. Whereas openness, agreeableness, and curiosity predicted positive expectancies of meditation, neuroticism predicted higher perceived barriers. Finally, we found little evidence that personality predicts self-selection into studies advertised as focussing on meditation.

Conclusions
Overall, these findings shed new light on personality traits that predict engagement in meditation practice and may help explain why different people seek out meditation. This knowledge may inform the development of tailored (and thereby more effective) meditation programs.

Preregistration
Both studies were preregistered on the Open Science Framework: https://osf.io/y32tk (Study 1), and https://osf.io/2qk64 (Study 2).
Modulation of test anxiety-induced salivary protein secretion by ovarian steroid hormones: a preliminary study
Authors: Zallocco Lorenzo , Ronci Maurizio , Pantalone Andrea , Mazzoni Maria Rosa , Ramoretti Eleonora , Lucacchini Antonio , Giusti Laura , Sebastiani Laura 
Published: February 2025

Abstract
In women the menstrual cycle influences mood and anxiety. Aim of this study was to preliminarily investigate whether different ovarian steroid hormone levels may modulate the psychophysiological responses elicited by test anxiety. Specifically, we compared the secretion of anxiety-induced salivary proteins of healthy women in the early follicular (Pre-Ov group) (low ovarian steroid hormones levels) and mid-luteal (Post-Ov group) (medium/high ovarian steroid hormones levels) phase of the menstrual cycle, during the simulation of an oral examination. Saliva samples were collected before and after a relaxation period and at two post-simulation times and analyzed by two-dimensional electrophoresis and western blot. Proteins corresponding to spots differentially expressed in the two groups across the session were identified through mass spectrometry and most of them corresponded to acute stress and/or oral mucosa immunity biomarkers. The task induced an increase in alpha-amylase, carbonic anhydrase and cystatin S, and a decrease in immunoglobulin light/J chains in both groups. Analogous changes in these proteins have previously been linked to psychological or physical stress. However, specific spots corresponding, for example, to cystatins and 14-3-3 protein, changed exclusively in the Pre-Ov group, while prolactin-inducible protein, polymeric immunoglobulin receptor, fragments of alpha-amylase and immunoglobulins only in the Post-Ov group, indicating a potential modulation of their secretion by ovarian steroid hormones. Overall, the results provide preliminary evidence that ovarian steroid hormones may be a driving factor for differences in physiological responses induced by test anxiety. The results are promising, but further validation in a larger sample is needed.
Navigating towards strengthened climate service processes
Authors: Wiréhn Lotten , Strandberg Gustav 
Published: February 2025

Abstract
Despite the importance of salient, credible, and legitimate climate information for climate action, studies demonstrate a persistent usability gap between the information provided and what users find relevant and useful. Drawing from scientific literature and our experiences working with a Swedish national climate service, we explore and reflect on challenges and barriers with climate services using an analytical framework of four pillars. Based on this, we provide four overarching recommendations (and fundamental needs): (i) Advancing data production and analysis, (ii) Establishing a climate service collaboration forum, (iii) Fostering active users, and (iv) Prioritising long-lasting funding. These recommendations are directed to policymakers and the climate service community to transition the production and use of climate information from short-term studies and initiatives to long-lasting processes. We argue that adopting these recommendations can support climate-resilient development through strengthening climate service infrastructure and enhancing capabilities and skills of the actors involved.
LapAppendectomy4all: validation of a new methodology for laparoscopic appendectomy simulation and training
Authors: Gonçalves Mário Rui , Marinho Ricardo , Reis Sofia Gaspar , Viveiros Ricardo , Teixeira Manuel Moutinho , Andrade Ana Kam , do Carmo Girão Maria , Rodrigues Pedro Pina-Vaz , Castelo-Branco Sousa Miguel 
Published: February 2025

Abstract
Appendectomy, whether open or minimally invasive (MIS) is one of the most frequent procedures performed by young residents. We designed and tested a new methodology and a new inexpensive silicone model for Laparoscopic Appendectomy (LA) simulation. This study aimed to assess their fidelity, usefulness and educational value in an introduction to laparoscopy course. The course was open to first-year general surgery residents. The group was divided in two and one of the groups watched a video of the procedure before the simulation. Individual performances were assessed directly on the models, using a specific assessment scale. Participants answered a questionnaire at the end of the course for evaluation. Thirty-five residents participated in this study. Execution, quality, and global performance were higher in the group that had more experience with the model. Thirty-two trainees (91%) answered the questionnaire. There was a strong agreement that the model was adequate for this type of course and face and content validity was considered high/very high. Participants strongly agreed that this model gives more confidence to perform a real LA and almost 97% (n = 31) considered they have learned solid foundations about LA. This study shows face, content and construct validation and also educational value for this new low-cost, laparoscopic appendectomy model. The integration of this model in an introduction to laparoscopy course showed good results in regard to an increase of confidence among first-year surgery residents. It can be a valuable tool for learning and training laparoscopic appendectomy.
Lessons from COVID-19 patient visitation restrictions: six considerations to help develop ethical patient visitor policies
Authors: Høeg Tracy Beth , Knudsen Benjamin , Prasad Vinay 
Published: February 2025

Abstract
Patient visitor restrictions were implemented in unprecedented ways during the COVID-19 pandemic and included bans on any visitors to dying patients and bans separating mothers from infants. These were implemented without high quality evidence they would be beneficial and the harms to patients, families and medical personnel were often immediately clear. Evidence has also accumulated finding strict visitor restrictions were accompanied by long-term individual and societal consequences. We highlight numerous examples of restrictions that were enacted during the COVID-19 pandemic, including some that continue to be in place today. We outline six specific concerns about the nature and effects of the visitor restrictions seen during the COVID-19 pandemic. These considerations may help provide both an ethical and science-based framework, through which healthcare workers, families and government entities can work towards safeguarding patient and family rights and well-being.
Complexity of chess domination problems
Authors: Langlois-Rémillard Alexis , Müssig Mia , Roldán Érika 
Published: February 2025

Abstract
We study different domination problems of attacking and non-attacking rooks and queens on polyominoes and polycubes of all dimensions. Our main result proves that maximum independent domination is NP-complete for non-attacking queens and for non-attacking rooks on polycubes of dimension three and higher. We also analyze these problems for polyominoes and convex polyominoes, conjecture the complexity classes, and provide a computer tool for investigation. We have also computed new values for classical queen domination problems on chessboards (square polyominoes). For our computations, we have translated the problem into an integer linear programming instance. Finally, using this computational implementation and the game engine Godot, we have developed a video game of minimum domination of queens and rooks on randomly generated polyominoes.
Australian guidance documents informing nature playspaces for children: a scoping review
Authors: Dankiw Kylie A. , Miller Nicole C. , Kumar Saravana , Baldock Katherine L. , Tsiros Margarita D. 
Published: February 2025

Abstract
In Australia, the absence of standard practice guidelines for nature playspace development prompts reliance on guidance documents from government agencies and nature play advocacy groups, posing challenges for end-users. Therefore, the aims of this review were to map recommendations for nature playspace creation/planning, design, implementation, and management within the Australian context. Ten databases and grey literature sources were searched from inception to August 2023, with inclusion criteria focusing on children aged < 18 years, recommendations describing nature playspace creation/planning, design, implementation, and management within the context of Australian outdoor playspace settings. Data extraction was performed in duplicate to identify recommendations for the creation, planning, design, implementation, and management of nature play spaces, followed by a descriptive synthesis of these recommendations. Out of 7,123 articles, 10 met inclusion criteria. The types of documents included were reports, general guides and one regulatory guideline. Nature play was described diversely, with commonalities in elements like trees, water, and plants, but inconsistencies in development approaches led to recommendations lacking clarity and detail. There was a lack of explicit detail regarding development context, suggesting a need for context-specific guidelines. While the included guidance documents provide initial frameworks for guiding nature play, they lack comprehensive, evidence-based recommendations for diverse contexts. Future research should focus on creating standard practice recommendations to ensure safe, age-appropriate, inclusive nature playspaces promoting health and developmental outcomes.
Superreflexive tensor product spaces
Authors: Rueda Zoca Abraham 
Published: February 2025

Abstract
The aim of this note is to prove that, given two superreflexive Banach spaces X and Y, then

$$X\widehat{\otimes }_\pi Y$$

X

⊗
^

π

Y

is superreflexive if and only if either X or Y is finite-dimensional. In a similar way, we prove that

$$X\widehat{\otimes }_\varepsilon Y$$

X

⊗
^

ε

Y

is superreflexive if and only if either X or Y is finite-dimensional.
Molecular mechanisms in liver repair and regeneration: from physiology to therapeutics
Authors: Ma Xiao , Huang Tengda , Chen Xiangzheng , Li Qian , Liao Mingheng , Fu Li , Huang Jiwei , Yuan Kefei , Wang Zhen , Zeng Yong 
Published: February 2025

Abstract
Liver repair and regeneration are crucial physiological responses to hepatic injury and are orchestrated through intricate cellular and molecular networks. This review systematically delineates advancements in the field, emphasizing the essential roles played by diverse liver cell types. Their coordinated actions, supported by complex crosstalk within the liver microenvironment, are pivotal to enhancing regenerative outcomes. Recent molecular investigations have elucidated key signaling pathways involved in liver injury and regeneration. Viewed through the lens of metabolic reprogramming, these pathways highlight how shifts in glucose, lipid, and amino acid metabolism support the cellular functions essential for liver repair and regeneration. An analysis of regenerative variability across pathological states reveals how disease conditions influence these dynamics, guiding the development of novel therapeutic strategies and advanced techniques to enhance liver repair and regeneration. Bridging laboratory findings with practical applications, recent clinical trials highlight the potential of optimizing liver regeneration strategies. These trials offer valuable insights into the effectiveness of novel therapies and underscore significant progress in translational research. In conclusion, this review intricately links molecular insights to therapeutic frontiers, systematically charting the trajectory from fundamental physiological mechanisms to innovative clinical applications in liver repair and regeneration.
USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer
Authors: Xie Zhongdong , Lin Hanbin , Wu Yuecheng , Yu Yanan , Liu Xintong , Zheng Yating , Wang Xiaojie , Wu Jiashu , Xu Meifang , Han Yuting , Zhang Qiongying , Deng Yu , Lin Lin , Linzhu Yan , Qingyun Li , Lin Xinjian , Huang Ying , Chi Pan 
Published: February 2025

Abstract
Metastasis is a major challenge for colorectal cancer (CRC) treatment. In this study, we identified autophagy activation as a prognostic indicator in CRC and observed that the expression of key autophagy proteins is elevated in metastatic and recurrent cases. Our subsequent goal was to identify potential genes associated with the autophagy panel and assess their prognostic significance, biological roles, and mechanisms in CRC metastasis. Among the candidates, CENPF emerged as the top gene in our screening process. We found that CENPF expression was preferentially elevated in CRC tissues compared to adjacent normal tissues, with significantly higher levels in CRC patients with tumor recurrence. Furthermore, a multicenter cohort study demonstrated that upregulated CENPF expression was strongly associated with poorer disease-free survival in CRC. Functional experiments showed that CENPF knockdown inhibited CRC cell invasion and metastasis both in vitro and in vivo. Intriguingly, we found CENPF undergoes degradation in CRC via the ubiquitination-proteasome pathway. Mechanistically, we observed that USP4 interacted with and stabilized CENPF via deubiquitination. Furthermore, USP4-mediated CENPF upregulation was critical regulators of metastasis of CRC. Examination of clinical samples confirmed that USP4 expression positively correlates with CENPF protein expression, but not mRNA transcript levels. Taken together, this study describes a novel USP4-CENPF signaling axis which is crucial for CRC metastasis, potentially serving as a therapeutic target and a promising prognostic biomarker for CRC.
Inhibition of lanosterol synthase linking with MAPK/JNK signaling pathway suppresses endometrial cancer
Authors: Ma Liangjian , Huang Wunan , Liang Xiaolei , Li Hongli , Yu Wei , Liu Lexin , Guan Yuelin , Liu Chang , Chen Xiangjun , Hu Lidan 
Published: February 2025

Abstract
Endometrial cancer (EC) is a significant health threat to women, with recurrence after treatment posing a major challenge. While abnormal cholesterol metabolism has been implicated in EC progression, the underlying mechanisms remain unclear. In this study, we identified lanosterol synthase (LSS) as a key mediator in cholesterol metabolism associated with EC. We found that LSS is significantly upregulated in EC tissues. Functional assays revealed that LSS promotes cell proliferation and migration, inhibits apoptosis, and drives tumor growth in vivo. Mechanistically, LSS exerts dual effects by accumulating cholesterol esters, thereby enhancing EC cell growth, and activating the MAPK/JNK signaling pathway. Importantly, inhibition of LSS with the specific inhibitor Ro 48-8071 not only reduced EC cell proliferation and suppressed xenograft tumor growth but also inhibited the growth of patient-derived tumor-like cell clusters (PTCs). These findings establish LSS as a novel oncogene in EC, promoting tumor progression through MAPK/JNK signaling activation and cholesterol ester accumulation, and highlight the therapeutic potential of targeting LSS in EC treatment.
Polarity-JaM: an image analysis toolbox for cell polarity, junction and morphology quantification
Authors: Giese Wolfgang , Albrecht Jan Philipp , Oppenheim Olya , Akmeriç Emir Bora , Kraxner Julia , Schmidt Deborah , Harrington Kyle , Gerhardt Holger 
Published: February 2025

Abstract
Cell polarity involves the asymmetric distribution of cellular components such as signalling molecules and organelles within a cell, alterations in cell morphology and cell-cell contacts. Advances in fluorescence microscopy and deep learning algorithms open up a wealth of unprecedented opportunities to characterise various aspects of cell polarity, but also create new challenges for comprehensible and interpretable image data analysis workflows to fully exploit these new opportunities. Here we present Polarity-JaM, an open source package for reproducible exploratory image analysis that provides versatile methods for single cell segmentation, feature extraction and statistical analysis. We demonstrate our analysis using fluorescence image data of endothelial cells and their collective behaviour, which has been shown to be essential for vascular development and disease. The general architecture of the software allows its application to other cell types and imaging modalities, as well as seamless integration into common image analysis workflows, see https://polarityjam.readthedocs.io. We also provide a web application for circular statistics and data visualisation, available at www.polarityjam.com, and a Napari plug-in, each with a graphical user interface to facilitate exploratory analysis. We propose a holistic image analysis workflow that is accessible to the end user in bench science, enabling comprehensive analysis of image data.