bluuebunny/crossref_metadata_2025_split huggingface dataset:
Personalized strategies for academic success in learning anatomy: Exploring metacognitive and technological adaptation in medical students
Authors: Stambuk‐Castellano Mónica , Carrera Anna , Tubbs R. Shane , Alario‐Hoyos Carlos , Verdú Enric , Iwanaga Joe , Reina Francisco 
Published: March 2024

AbstractPersonalization of learning is an educational strategy rooted in metacognition and is significant in academic training. This is especially true in medical contexts. This study explored the relationship between the metacognitive profile of students of human anatomy, the classification of questions according to their difficulty, and the anatomical domain. It also covered the integration of educational technologies to create personalized learning environments. The identification of metacognitive profiles (“Active”, “Pragmatic”, “Theoretical”, and “Reflective”) has been highlighted as a critical influence on students' responses to different pedagogical approaches. Personalized adaptation based on these profiles has shown potential for improving grades and increasing student satisfaction and engagement with learning. The results revealed variations in student performance in relation to different pedagogical approaches, learning units, and evaluation modalities. The “Experience” evaluation modality, personalized according to metacognitive profiles, level of competence, and learning objectives, resulted in higher average scores. However, there was significant variability in the results. Those findings confirm the effectiveness of metacognitive adaptation in improving academic performance. Furthermore, they provide a solid basis for formulating personalized and effective pedagogical strategies in medical education. They recognize the influence of metacognitive profiles on student performance and contribute to advancing medical pedagogy.
Sex differences in the outcomes after transcatheter aortic valve replacement with newer generation devices: A meta‐analysis
Authors: El Sherbini Adham H. , Servito Maria , Zidan Ali , Elsherif Salma , Ko Grace , Elgendy Islam Y. , Ya'Qoub Lina 
Published: March 2024

AbstractBackgroundTranscatheter aortic valve replacement (TAVR) is a reasonable therapeutic approach among patients with symptomatic severe aortic stenosis irrespective of surgical risk. Data regarding sex‐specific differences in the outcomes with newer generation valves are limited.MethodsElectronic databases were searched for studies assessing sex differences in the outcomes of patients undergoing TAVR with newer generation valves (SAPIEN 3 or Evolut). Random effects model was constructed for summary estimates.ResultsFour observational studies with 4522 patients (44.8% women) were included in the meta‐analysis. Women were older and had a lower prevalence of coronary artery disease and mean EuroScore. Women had a higher incidence of short‐term mortality (up to 30 days) (risk ratio [RR]: 1.60, 95% confidence interval [CI]: 1.14–2.25), but no difference in 1‐year mortality (RR: 0.92, 95% CI: 0.72–1.17). There was no significant difference in the incidence of major bleeding (RR: 1.16, 95% CI: 0.86–1.57), permanent pacemaker (PPM) (RR: 0.80, 95% CI: 0.62–1.04), or disabling stroke (RR: 1.16, 95% CI: 0.54–2.45).ConclusionIn this meta‐analysis, we found that women undergoing TAVR with newer‐generation devices were older but had a lower prevalence of comorbidities. Women had a higher incidence of short‐term mortality but no difference in the 1‐year mortality, bleeding, PPM, or stroke compared with men. Future studies are required to confirm these findings.
Non‐invasive assessment of hepatic steatosis by ultrasound‐derived fat fraction in individuals at high‐risk for metabolic dysfunction‐associated steatotic liver disease
Authors: Tavaglione Federica , Flagiello Valentina , Terracciani Francesca , Gallo Paolo , Capparelli Emma , Spiezia Chiara , De Vincentis Antonio , Palermo Andrea , Scriccia Sara , Galati Giovanni , Napoli Nicola , Daniels Samuel J. , Blau Jenny E. , Carlsson Björn , Khazrai Yeganeh M. , Incalzi Raffaele Antonelli , Picardi Antonio , Vespasiani‐Gentilucci Umberto 
Published: March 2024

AbstractAimsGiven the increasing number of individuals developing metabolic dysfunction‐associated steatotic liver disease (MASLD) and the low rate of those with progressive liver disease, there is a pressing need to conceive affordable biomarkers to assess MASLD in general population settings. Herein, we aimed to investigate the performance of the ultrasound‐derived fat fraction (UDFF) for hepatic steatosis in high‐risk individuals.MethodsA total of 302 Europeans with obesity, type 2 diabetes, or a clinical history of hepatic steatosis were included in the analyses. Clinical, laboratory, and imaging data were collected using standardized procedures during a single screening visit in Rome, Italy. Hepatic steatosis was defined by controlled attenuation parameter (CAP) or ultrasound‐based Hamaguchi's score. UDFF performance for hepatic steatosis was estimated by the area under the receiver operating characteristic curve (AUC).ResultsOverall, median (IQR) UDFF was 12% (7–20). UDFF was positively correlated with CAP (ρ = 0.73, p < 0.0001) and Hamaguchi's score (ρ = 0.79, p < 0.0001). Independent predictors of UDFF were circulating triglycerides, alanine aminotransferase (ALT), and ultrasound‐measured visceral adipose tissue (VAT). UDFF AUC was 0.89 (0.85–0.93) and 0.92 (0.88–0.95) for CAP‐ and ultrasound‐diagnosed hepatic steatosis, respectively. UDFF AUC for hepatic steatosis was higher than those of fatty liver index (FLI), hepatic steatosis index (HSI), CAP‐score (CAPS), and ALT (p < 0.0001). Lower age, ALT, and VAT were associated with discordance between UDFF and ultrasound.ConclusionsUDFF may be a simple and accurate imaging biomarker to assess hepatic steatosis and monitor changes in hepatic fat content over time or in response to therapeutic interventions beyond clinical trials.
Myb overexpression synergizes with the loss of Pten and is a dependency factor and therapeutic target in T‐cell lymphoblastic leukemia
Authors: Almeida André , T'Sas Sara , Pagliaro Luca , Fijalkowski Igor , Sleeckx Wouter , Van Steenberge Hannah , Zamponi Raffaella , Lintermans Béatrice , Van Loocke Wouter , Palhais Bruno , Reekmans Alexandra , Bardelli Valentina , Demoen Lisa , Reunes Lindy , Deforce Dieter , Van Nieuwerburgh Filip , Kentsis Alex , Ntziachristos Panagiotis , Van Roy Nadine , De Moerloose Barbara , Mecucci Cristina , La Starza Roberta , Roti Giovanni , Goossens Steven , Van Vlierberghe Pieter , Pieters Tim 
Published: March 2024

AbstractT‐lineage acute lymphoblastic leukemia (T‐ALL) is an aggressive hematological malignancy that accounts for 10%–15% of pediatric and 25% of adult ALL cases. Although the prognosis of T‐ALL has improved over time, the outcome of T‐ALL patients with primary resistant or relapsed leukemia remains poor. Therefore, further progress in the treatment of T‐ALL requires a better understanding of its biology and the development of more effective precision oncologic therapies. The proto‐oncogene MYB is highly expressed in diverse hematologic malignancies, including T‐ALLs with genomic aberrations that further potentiate its expression and activity. Previous studies have associated MYB with a malignant role in the pathogenesis of several cancers. However, its role in the induction and maintenance of T‐ALL remains relatively poorly understood. In this study, we found that an increased copy number of MYB is associated with higher MYB expression levels, and might be associated with inferior event‐free survival of pediatric T‐ALL patients. Using our previously described conditional Myb overexpression mice, we generated two distinct MYB‐driven T‐ALL mouse models. We demonstrated that the overexpression of Myb synergizes with Pten deletion but not with the overexpression of Lmo2 to accelerate the development of T‐cell lymphoblastic leukemias. We also showed that MYB is a dependency factor in T‐ALL since RNA interference of Myb blocked cell cycle progression and induced apoptosis in both human and murine T‐ALL cell lines. Finally, we provide preclinical evidence that targeting the transcriptional activity of MYB can be a useful therapeutic strategy for the treatment of T‐ALL.
Usefulness of KIT mutant transcript levels for monitoring measurable residual disease in t (8;21) acute myeloid leukemia
Authors: Sun Yuan , Wang Xu , Chen Wen‐Min , Hao Yue , Li Ling‐Di , Li Jin‐Ying , Sun Kai , Shi Zong‐Yan , Jiang Hao , Jiang Qian , Huang Xiao‐Jun , Qin Ya‐Zhen 
Published: March 2024

AbstractIn addition to RUNX1::RUNX1T1 transcript levels, measurable residual disease monitoring using KIT mutant (KITmut) DNA level is reportedly predictive of relapse in t (8; 21) acute myeloid leukemia (AML). However, the usefulness of KITmut transcript levels remains unknown. A total of 202 bone marrow samples collected at diagnosis and during treatment from 52 t (8; 21) AML patients with KITmut (D816V/H/Y or N822K) were tested for KITmut transcript levels using digital polymerase chain reaction. The individual optimal cutoff values of KITmut were identified by performing receiver operating characteristics curve analysis for relapse at each of the following time points: at diagnosis, after achieving complete remission (CR), and after Course 1 and 2 consolidations. The cutoff values were used to divide the patients into the KITmut‐high (KIT_H) group and the KITmut‐low (KIT_L) group. The KIT_H patients showed significantly lower relapse‐free survival (RFS) and overall survival (OS) rates than the KIT_L patients after Course 1 consolidation (p = 0.0040 and 0.021, respectively) and Course 2 consolidation (p = 0.018 and 0.011, respectively) but not at diagnosis and CR. The <3‐log reduction in the RUNX1::RUNX1T1 transcript levels after Course 2 consolidation was an independent adverse prognostic factor for RFS and OS. After Course 2 consolidation, the KIT_H patients with >3‐log reduction in the RUNX1::RUNX1T1 transcript levels (11/45; 24.4%) had similar RFS as that of patients with <3‐log reduction in the RUNX1::RUNX1T1 transcript levels. The combination of KITmut and RUNX1::RUNX1T1 transcript levels after Course 2 consolidation may improve risk stratification in t (8; 21) AML patient with KIT mutation.
Bioactive poly(salicylic acid)‐poly(citric acid) scaffolds improve diabetic wound repair via regulating HIF‐1α, Nrf2 and macrophage
Authors: Xie Chenxi , Ma Junping , Luo Meng , Wang Yidan , Lei Bo 
Published: March 2024

AbstractDiabetic wounds environment is over‐oxidized, over‐inflammatory, leading to difficulties in regenerating blood vessels, and retardation of healing in diabetic wounds. Therefore, diabetic wounds can be treated from the perspective of scavenging oxidative free radicals and reducing the level of inflammation. Herein, we report a bioactive poly(salicylic acid)‐poly(citric acid) (FPSa‐PCG) hydrogel for diabetic wound repair. The FPSa‐PCG hydrogel shows abilities of antioxidation, anti‐inflammation, and regulation of macrophage phenotype. The FPSa‐PCG hydrogel showed good biocompatibility, and obtain the abilities of promotion of macrophages migration, reduction of ROS generation, suppression of the M1‐type macrophage polarization. FPSa and PCG could synergistically enhance the angiogenesis through upregulating the mRNA expression of HIF1Α, VEGF, and CD31 in endothelial cells and reduce the ROS level of macrophages through upregulating the mRNA expression of Nrf2. The in vivo diabetic wound model confirmed the promoting effect of FPSa‐PCG hydrogel on wound closure in diabetes. The further studies found that FPSa‐PCG hydrogel could induce the CD31 protein expression in the subcutaneous tissue and inhibit the TNF‐a protein expression. This work shows that the simple composition FPSa‐PCG hydrogel has a promising therapeutic potential in the treatment of diabetic wounds.
Preparation of calcium phosphate ion clusters through atomization method for biomimetic mineralization of enamel
Authors: Dong Haide , Qiu Lin , Zhu Chen , Fan Wuzhe , Liu Li , Deng Quanfu , Zhang Huan , Yang Weihu , Cai Kaiyong 
Published: March 2024

AbstractDental enamel is a mineralized extracellular matrix, and enamel defect is a common oral disease. However, the self‐repair capacity of enamel is limited due to the absence of cellular components and organic matter. Efficacy of biomimetic enamel mineralization using calcium phosphate ion clusters (CPICs), is an effective method to compensate for the limited self‐healing ability of fully developed enamel. Preparing and stabilizing CPICs presents a significant challenge, as the addition of certain stabilizers can diminish the mechanical properties or biosafety of mineralized enamel. To efficiently and safely repair enamel damage, this study quickly prepared CPICs without stabilizers using the atomization method. The formed CPICs were evenly distributed on the enamel surface, prompting directional growth and transformation of hydroxyapatite (HA) crystals. The study revealed that the mended enamel displayed comparable morphology, chemical composition, hardness, and mechanical properties to those of the original enamel. The approach of repairing dental enamel by utilizing ultrasonic nebulization of CPICs is highly efficient and safe, therefore indicating great promise.
Electron‐translucency and partial defects of synaptic basal lamina in the electrocyte synapse of an electric ray (Narke japonica) in 3D embedment‐free section electron microscopy
Authors: Chomphoo Surang , Kondo Hisatake , Hipkaeo Wiphawi 
Published: March 2024

AbstractThe synaptic basal lamina of the electrocytes was disclosed to be electron‐translucent to some extent when viewed in an en‐face direction in embedment‐free section transmission electron microscopy (EFS‐TEM), and synaptic vesicles located close to the presynaptic membrane were seen through the synaptic basal lamina together with the presynaptic and postsynaptic membranes. This feature of translucency has the potential to analyze possible spatial interrelations in situ between bioactive molecules in the synaptic basal lamina and the synaptic vesicles in further studies. The synaptic basal lamina, appearing as an electron‐dense line sandwiched by two parallel lines representing the presynaptic and postsynaptic membranes in ultrathin sections cut right to the synaptic junctional plane in conventional TEM, was not fully continuous but randomly intermittent along its trajectory. Compatible with the intermittent line appearance, the en‐face 3D view in embedment‐free section TEM revealed for the first time partial irregular defects of the synaptic basal lamina. Considering the known functional significance of several molecules contained in the synaptic basal lamina in the maintenance and exertion of the synapse, its partial defects may not represent its rigid structural features, but its immature structure under remodeling or its dynamic changes in consistency such as the sol/gel transition, whose validity needs further examination.Research Highlights
In embedment‐free section TEM, a 3D en‐face view of synaptic basal lamina in situ is reliably possible.
The basal lamina en‐face is electron‐translucent, which makes it possible to analyze spatial interrelation between pre‐ and post‐synaptic components.
Partial irregular defects in the basal lamina are revealed in Torpedo electrocytes, suggesting its remodeling or dynamic changes in consistency.
A cadaveric study of the posterior band of the inferior glenohumeral ligament of the shoulder and its dynamic behaviour in different arm positions
Authors: Nourissat Geoffroy , Bastard Claire , Cammas Claire , Salabi Vincent , Billaud Anselme 
Published: March 2024

AbstractPurposeThe inferior glenohumeral ligament (IGHL) is composed of three parts: the anterior branch or band (AB), the axillary pouch and the posterior band (PB). The latter has rarely been studied. We aim to describe the PB of the IGHL and its dynamic behaviour in different arm positions.MethodsTwelve fresh cadaveric shoulders were used and the two bands (AB and PB) of the IGHL were dissected and isolated, taking away all muscle, ligaments and capsule. Characteristics of the bands were studied in five positions: maximum external rotation (ER1), abduction (ABD), internal rotation (IR), ABD external rotation (ER2) and anterior elevation–adduction–IR (Hawkins–Kennedy test position). Progressive and randomized sectioning of the bands and capsule with a scalpel was performed to study its impact on mobility and translation of the glenohumeral joint.ResultsThe bands that tensioned first were in ER1, the AB at 97 ± 9° (80–110); in ER2, the AB at 81 ± 19° (30–100); in IR, the PB at 64 ± 9° (50–80); and in ABD, the PB at 87 ± 10° (70–105). Isolated sectioning of the AB had no effect on ABD, whilst isolated sectioning of the PB allowed greater ABD. In ER2, the AB limited anterior translation. After sectioning the AB, anterior translation remained limited by the PB, which wrapped around the humeral head and locked the joint by pressing the two joint surfaces tightly together. In Hawkins–Kennedy position anterior elevation–adduction–IR, the AB is the first constraint and the posterior translation was limited by the PB alone only in four cases.ConclusionsWhen the IGHL is isolated, ligament limitation of glenohumeral ABD seems to be uniquely dependent on the PB. In the Hawkins and Kennedy position, the AB is the first constraint. In the case of an isolated lesion to the AB, the PB participates in anterior stabilization of the shoulder by wrapping around the humeral head that cannot dislocate. These findings confirm the role of the PB in glenohumeral joint stability.Level of EvidenceLevel IV.
Cigarette smoke extract exacerbates progression of osteoarthritic‐like changes in cartilage explant cultures
Authors: DiNicola Emily Sawvell , Martinez Andrea Vera , Walker Lizzie , Wu Yongren , Burnikel Brian G. , Mercuri Jeremy 
Published: March 2024

AbstractEstablished risk factors for osteoarthritis (OA) include obesity, joint injury, age, race, and genetics. However, the relationship between cigarette smoking and OA has yet to be established. In the present study, we have employed the use of cigarette smoke extract (CSE), the water‐soluble vapor phase of cigarette smoke, with porcine cartilage explants to investigate the effects of cigarette smoking on cartilage catabolism at the tissue level. Articular cartilage explants were first exposed to 2.5%, 5%, and 10% CSE to assess its effects on cartilage homeostasis. Following, the effects of CSE on OA‐like inflammation was observed by culturing explants with a combined treatment of IL‐1β and TNF‐α and 10% CSE (CSE + OA). Cartilage explants were assessed for changes in viability, biochemical composition, extracellular matrix (ECM) integrity, and equilibrium mechanical properties (aggregate modulus and hydraulic permeability). CSE alone leads to both a time‐ and dose‐dependent decrease in chondrocyte viability but does not significantly affect sGAG content, percent sGAG loss, or the ECM integrity of cartilage explants. When IL‐1β and TNF‐α were combined with 10% CSE, this led to a synergistic effect with more significant losses in viability, significantly more sGAG loss, and significantly higher production of ROS than OA‐like inflammation only. Cartilage explant equilibrium mechanical properties were unaffected. Within the timeframe of this study, CSE alone does not cause OA but when combined with OA‐like inflammation leads to worsened articular cartilage degeneration as measured by chondrocyte viability, sGAG loss, proteoglycan staining, and ROS production.
Fully automatic quantitation of eight different metabolites in coffee using 1H‐NMR spectroscopy and the PULCON methodology
Authors: Gottstein Vera , Lachenmeier Dirk W. , Kuballa Thomas , Bunzel Mirko 
Published: March 2024

AbstractBackgroundCoffee contains a plethora of constituents with some of them being especially important either due to their physiological effects or as quality markers. As quantitative proton nuclear magnetic resonance spectroscopy (1H‐NMR) has been established as a fast and reliable analytical tool its application was evaluated for the simultaneous quantitation of lactic acid, acetic acid, formic acid, caffeine, caffeoylquinic acid (CQA) isomers, N‐methylpyridinium, trigonelline, and 5‐hydroxymethylfurfural (HMF) in aqueous extracts of roasted Coffea arabica samples.ResultsSimultaneous quantitative determination was achieved by an automated analysis based on the PULCON methodology (pulse length‐based concentration determination). The method was validated regarding linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ). Recovery rates were between 76% (CQA) and 116% (HMF), and precision was between 1.7% (caffeine) and 10.3% (HMF). The LOD varied between 0.06 g/kg (HMF) and 1.35 g/kg (caffeine and CQA), with the LOQ being between 0.22 g/kg (HMF) and 4.87 g/kg (CQA). To verify the results of the 1H‐NMR method, caffeine, trigonelline, HMF, 3‐CQA, 4‐CQA, and 5‐CQA were additionally quantitated by HPLC‐DAD and the results were compared. The described 1H‐NMR method was additionally applied to coffee samples that contained different coffee defects. Results showed only slight changes in the concentrations of the analytes by adding defective beans to defect‐free coffee.DiscussionThe developed 1H‐NMR approach was proven to be fast (30 min), reliable, and precise. Thus, it is well suited to analyze several coffee constituents of interest in a large number of samples in, for example, quality control.
Caliper‐verified unrestricted kinematically aligned total knee arthroplasty in Asian patients showed efficacious mid‐ to long‐term results regardless of postoperative alignment categories
Authors: Won Seok‐Hyung , Eim Seong‐Hyuk , Shen Quan‐Hu , Kim Kwang‐Kyoun , Won Ye‐Yeon 
Published: March 2024

AbstractPurposeThere have been insufficient data regarding the long‐term results of unrestricted kinematically aligned total knee arthroplasty (unKATKA) in Asian patients. We investigated mid‐ to long‐term clinical and radiological follow‐up data of Korean patients after caliper‐verified unKATKA of minimum 7.4–10 years including categorised data of postoperative tibial component, limb and knee alignment. Additionally, we analysed the preoperative distribution and postoperative restoration of coronal plane alignment of knee (CPAK) phenotypes.MethodsThis study is a retrospective analysis of 63 patients: 96 osteoarthritic underwent consecutive caliper‐verified unKATKA between October 2013 and May 2016 by a single surgeon. Implant survivorship was investigated for revision for any reason. Each knee was categorised into an in‐range or outlier group by three postoperative alignment parameters: tibial component, knee and limb alignment. Statistical analyses were done for any significant differences in clinical scores and implant survival rates between groups. Finally, all knees were classified into CPAK classification postoperatively and postoperatively. The CPAK restoration rate was calculated.ResultsAmong 85 knees in the clinically confirmed group, implant survival was 98.8%. There was one case of revision due to periprosthetic fracture. The percentage postoperatively aligned in the varus (valgus) outlier range was 100% (0%) for tibial component, 16.7% (24.8%) for the knee alignment and 51% (0%) for the limb alignment. All three categories did not affect implant survival or clinical scores. Eighty one out of 96 knees (84.4%) were restored to their CPAK phenotype postoperatively.ConclusionWith the limitation of a case series having a small number of patients and gender deviation, our study suggests that caliper‐verified unKATKA could be a good option regardless of geographical variation of constitutional alignment in patients with osteoarthritis (OA).Level of EvidenceLevel IV, therapeutic study.