bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prenatal sonographic features of trisomy 1q
Authors: Wax Joseph R. , Carpenter Molly , Chard Renée , Cartin Angelina , Pinette Michael G. , Blackstone Jacquelyn 
Published: July 2007

AbstractWe describe the sonographic features of trisomy 1q in 2 affected fetuses and identify 17 other published reports of this entity in the literature. Four of 5 (80%) diagnoses made at ≤14 weeks' gestation demonstrated increased nuchal translucency or cystic hygroma colli. During the second and third trimesters, findings included cerebral ventriculomegaly (n = 8 [57%]), nuchal skin fold ≥6 mm or cystic hygroma colli (n = 5 [36%]), urinary anomalies (n = 5 [36%]), digit malformations (n = 5 [36%]), and abnormal amniotic fluid volume (n = 6 [40%]). Findings in trisomy 1q may be influenced by coexisting chromosomal deletions or mosaicism. Sonographic features generally reflect the location and size of the 1q duplication. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2008
Plantar keratoderma as a presenting sign of pancreatic adenocarcinoma
Authors: Ulla Jose Luis , Garcia‐Doval Ignacio , Posada Celia , Soto Santiago , Alvarez Carlos , Ledo Luis , Vazquez‐Astray Enrique 
Published: July 2007

AbstractA 76‐year‐old woman presented with a 1‐year history of back pain and sudden onset of plantar keratoderma. Her serum carbohydrate antigen (CA 19.9) levels were elevated. Endoscopic radial sonographic examination led to the diagnosis of pancreatic adenocarcinoma, in a stage not detectable with helical CT yet amenable to surgical therapy. Cutaneous lesions disappeared after distal pancreatectomy. © 2007 Wiley Periodicals, Inc. J Clin Ultrasound, 2008
Cell‐permeant cytoplasmic blue fluorophores optimized for in vivo two‐photon microscopy with low‐power excitation
Authors: Hayek Ali , Grichine Alexei , Huault Thomas , Ricard Clément , Bolze Frédéric , Van Der Sanden Boudewijn , Vial Jean‐Claude , Mély Yves , Duperray Alain , Baldeck Patrice Lilian , Nicoud Jean‐François 
Published: July 2007

AbstractBecause of the spreading of nonlinear microscopies in biology, there is a strong demand for specifically engineered probes in these applications. Herein, we report on the imaging properties in living cells and nude mice brains of recently developed water soluble blue fluorophores that show efficient diffusion through cell membranes and blood–brain barriers. They are characterized by two‐photon absorption cross‐sections of 100–150 Goeppert‐Mayer range in the near IR and fluorescence efficiencies of up to 72% in water. They were found to stain homogeneously the cytoplasm of cultured living cells within minutes. Moreover, their diffusion times and fluorescence characteristics in the cytoplasm suggest a hydrophobic association with intracellular membranes. Their intracellular fluorescent decays were found to be almost mono‐exponential, a very favorable feature for fluorescence lifetime imaging. Two photon images of living cells were obtained with a good signal to noise ratio using laser powers in the sub‐milliwatt range. This allows continuous imaging without significant photobleaching for tens of minutes. In addition, these fluorophores allowed in vivo three‐dimensional two‐photon imaging of mice cortex vasculatures and extra vasculature structures, with no sign of toxicity. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
In situ hybridization studies confirming recent findings of the existence of a local nonneuronal catecholamine production in human patellar tendinosis
Authors: Danielson Patrik , Alfredson Håkan , Forsgren Sture 
Published: July 2007

AbstractWe have in recent studies presented unexpected immunohistochemical evidence favoring the existence of a local production of catecholamines, and an occurrence of adrenergic receptors on the tendon cells (tenocytes), in the human patellar tendon. This was particularly noticed for tendons from patients suffering from tendinosis (chronic tendon pain), which has led us to propose an involvement of this autocrine/paracrine system in the development of tendinosis, especially since catecholamines have been reported to be modulators of tissue remodeling and pain processes. However, the findings concerning catecholamine production have so far only been noted at the level of protein detection, and for this reason, the aim of the present study was to confirm the previous immunohistochemical results by using in situ hybridization (ISH) technique. A ssDNA probe detecting human mRNA for the catecholamine‐synthesizing enzyme tyrosine hydroxylase (TH) was applied. The ISH results revealed that there were clear reactions indicating the existence of mRNA for TH in tenocytes of tendinosis specimens. It was generally noted that disfigured tenocytes were the ones with the most distinct reactions, while normally looking tenocytes hardly displayed any reactions at all. In conclusion, this study presents the first evidence at the mRNA level of the existence of a local nonneuronal production of catecholamines in human patellar tendon tissue. The findings add to recent observations of the occurrence of a local production in tendons of signal substances traditionally related to neurons. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
The influences of macrosegregation, intermetallic particles, and dendritic spacing on the electrochemical behavior of hypoeutectic Al‐Cu alloys
Authors: Osório Wislei R. , Spinelli José E. , Boeira Alexandre P. , Freire Célia M. , Garcia Amauri 
Published: July 2007

AbstractThe purpose of this research is (1) to investigate the influence of Al2Cu intermetallic particles associated with the dendritic arm spacing on the corrosion resistance of different hypoeutectic Al‐Cu alloys and (2) to evaluate the electrochemical behavior of a hypoeutectic Al‐Cu alloy directionally solidified under unsteady‐state heat flow. The as‐cast samples were produced using vacuum arc remelting and vertical upward water‐cooled solidification. Microscopic examinations were carried out with optical microscopy and scanning electron microscopy + energy dispersiveX‐ray analyses. To evaluate the surface corrosion behavior of such alloys, all corrosion tests were performed in a 0.5‐M NaCl solution at 25°C using an electrochemical impedance spectroscopy technique and potentiodynamic polarization curve analysis. Based on the tests, corrosion rate and impedance parameters were obtained. The present research has underlined the use of appropriate techniques of characterization for determining Al2Cu distribution, morphology, and fraction within the typical microstructures of Al‐Cu alloys. The experimental results have established correlations between the Al‐rich phase dendritic arm size, the intermetallic particles distribution in the eutectic mixture, the macrosegregation profile, and the resulting corrosion resistance. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Kinetics of TmHU binding to DNA as observed by optical tweezers
Authors: Salomo Mathias , Keyser Ulrich F. , Kegler Kati , Gutsche Christof , Struhalla Marc , Immisch Claudia , Hahn Ulrich , Kremer Friedrich 
Published: July 2007

AbstractThe kinetics of binding for the histone‐like protein TmHU (from Thermotoga maritima) to DNA is analyzed on a single molecule level by use of optical tweezers. For the reaction rate a pronounced concentration‐dependence is found with an “all or nothing”‐limit which suggests the cooperative nature of the binding‐reaction. By analyzing the statistics of mechanically induced dissociation‐events of TmHU from DNA multiple reaction sites are observed to become more likely with increasing TmHU concentration. This is interpreted as a hint for a secondary organizational level of the TmHU/DNA complex. The reaction rate of TmHU binding to DNA is remarkably higher than that of the HU protein from Escherichia coli which will be discussed. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Trabecular bone microarchitecture is related to the number of risk factors and etiology in osteoporotic men
Authors: Legrand Erick , Audran Maurice , Guggenbuhl Pascal , Levasseur Régis , Chalès Gérard , Baslé Michel‐Félix , Chappard Daniel 
Published: July 2007

AbstractMicroarchitecture of trabecular bone is a very important component of bone quality in osteoporosis and a determinant of vertebral fracture in men with low bone mineral density (BMD). In contrast to women, male osteoporosis is, in most cases, secondary. The relationships between microarchitecture and different risk factors have never been evaluated in men. About 152 men with low BMD at the lumbar spine or hip (BMD, T‐score < −2.5) were included in this study. Risk factors were: age, BMI, alcohol intake, corticosteroid therapy, hypogonadism, and chronic diseases. Transiliac bone biopsies were obtained and histomorphometry was done on an image analyzer; the following parameters were measured: cortical thickness (Ct.Th), trabecular bone volume (BV/TV), trabecular thickness (Tb.Th), separation (Tb.Sp) and number (Tb.N), interconnectivity Index (ICI), star volume of the bone marrow, and strut analysis with node and free‐end count. The 50 men with two risk factors had a lower BMD, lower Ct.Th and a significant higher star volume than those with one factor or idiopathic osteoporosis. The 26 men with at least three risk factors, had a lower BMD, a reduction of BV/TV and Ct.Th and a marked disorganization of the trabecular network (increased Tb.Sp, ICI, star volume, and free‐end to free‐end struts). The prevalence of vertebral fractures was higher in these patients. When the main risk factor was considered, a marked decrease in trabecular bone connectivity was observed in hypogonadic men. In osteoporotic men, higher the number of risk factors, lower the connectivity of trabecular network and higher the vertebral fracture risk. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Attachment and entry of Candida famata in monocytes and epithelial cells
Authors: Pacheco María , Pisa Diana , García‐Gómez Patricia , Carrasco Luis , Juarranz Ángeles 
Published: July 2007

AbstractCandida albicans is considered the main pathogenic yeast responsible for a multitude of infective disorders. However, other yeasts, such as Candida famata, are being recognized as potential emerging pathogens that cause several types of infections in humans and animals. Consequently, we have investigated the adhesion and internalization of Candida famata into monocytes and epithelial cells. The interaction of the yeast with the cells is very rapid and takes place during the first 15 min of injection. However, the affinity of the yeast for the cells varies, THP‐1 (human monocytes) being the highest and followed in decreasing order by HeLa (human carcinoma), HaCaT, and Pam‐212 (human and mouse keratinocytes, respectively). Heat inactivation or treatment with nystatin, significantly decreases yeast adhesion to cells. Immunofluorescence, as well as scanning and transmission electron microscopy, reveals that cell lines are able to internalize C. famata. At 48 h after infection, most of the yeasts located inside cells appear degraded, but some yeasts recovered from lysed cells, were still viable. Adhesion and internalization of C. famata into HeLa cells were found to be lower than those of C. albicans and C. glabrata, but higher than those of S. cerevisiae. In addition, infection with C. famata results in actin microfilaments rearrangement. This article presents novel data in the interaction of this pathogenic yeast with mammalian cells. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Reversible photoswitching enables single‐molecule fluorescence fluctuation spectroscopy at high molecular concentration
Authors: Eggeling C. , Hilbert M. , Bock H. , Ringemann C. , Hofmann M. , Stiel A.C. , Andresen M. , Jakobs S. , Egner A. , Schönle A. , Hell S.W. 
Published: July 2007

AbstractWe demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing entities, because the equilibrium between the fluorescent ON‐ and the dark OFF‐state can be shifted through optical irradiation at a specific wavelength. Depending on the irradiation intensity, the concentration of the ON‐state markers can be up to 1,000 times lower than the actual concentration of the labeled molecular entity. Photoswitching expands the range of single‐molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
Modification of aluminum chips for LDI mass spectrometry of proteins
Authors: Shmanai Vadim , Gontarev Sergey , Frey Simone K. , Schweigert Florian J. 
Published: July 2007

AbstractMatrix‐assisted laser desorption ionization time‐of‐flight mass spectroscopy (MALDI TOFMS) combined with affinity chromatography on immobilized phenylboronic acid agarose gels was used for selective enrichment and detection of specifically modified proteins such as glycated proteins in complex biological samples. Physicochemical grafting of hydrophilic polymers on aluminum surface was developed to reduce nonspecific protein sorption and to create a proper support layer for a three‐dimensional affinity hydrogel. Grafted agarose allowed the fixation of three‐dimensional agarose hydrogel on the chip surface. Both pinched polymers and hydrogels were effectively derivatized. 3‐Aminophenylboronic acid (mPBA) was covalently immobilized as an affinity ligand to achieve specific binding of glycated plasma proteins. Alternatively, the affinity sorbent was immersed into the hydrogel to increase binding capacity. MALDI TOFMS was used to evaluate binding efficiency and molecular mass changes of human serum albumin due to glycation. Glycated proteins were captured directly on the chip with high selectivity and efficacy, and low nonspecific binding. Thus they could easily be characterized by MALDI TOFMS. Copyright © 2007 John Wiley & Sons, Ltd.
p‐Nitro‐N‐propylaniline/silica: Synthesis, characterization, and its application in matrix solid phase dispersion for multiresidue analysis of pesticides in carrots
Authors: Contieri Abad Fernanda , Winck Priscila Romero , Benvenutti Edilson Valmir , Carmo Ruaro Peralba Maria do , Bastos Caramão Elina , Alcaraz Zini Cláudia 
Published: July 2007

AbstractA new material for matrix solid phase dispersion (MSPD) was synthesized – p‐nitro‐N‐propylaniline/silica (pNNPASi) by grafting reactions, characterized by elemental analysis and N2‐adsorption‐desorption isotherms, and tested for multiclass multiresidue analysis of pesticides in wet and freeze‐dried carrots. Results obtained applying this new solid phase sorbent to MSPD extraction of ten pesticides (trichlorphon, trifluralin, dicloran, chlorothalonil, prometryn, linuron, captan, procymidone, prochloraz, and deltametrin) in wet carrots showed better results than the ones obtained for freeze‐dried samples. Recoveries were in the range of 48–106% and precisions varied from 6 to 20% when wet samples were employed. Comparison between pNNPASi sorbent and C18 showed better performance of pNNPASi for eight out of ten pesticides tested. The LOQs show that the developed method can be used to detect the pesticides investigated in carrots at concentrations below the maximum residue levels (MRL) established by EU, USEPA, and National Sanitary Surveillance Agency (ANVISA). Linuron, captan, prochloraz, and deltamethrin were found in at least one of the two commercial samples studied in concentrations above the LOQ of this method. Concentrations of the last three pesticides were above the European MRL in one of the commercial samples.
Elements of innovative cultures
Authors: Dombrowski Caroline , Kim Jeffrey Y. , Desouza Kevin C. , Braganza Ashley , Papagari Sridhar , Baloh Peter , Jha Sanjeev 
Published: July 2007

AbstractOrganizational culture is an important determinant of sustained innovativeness and financial performance. Though it is easy to appreciate the important role culture plays in making an innovation successful, it is difficult to change culture. One way of changing culture could be to identify elements of innovative culture and then imbibing the ones relevant to a given organization. In this paper, we have identified, based on past research, eight elements of organizational innovative culture: innovative mission and vision statements, democratic communication, safe spaces, flexibility, collaboration, boundary spanning, incentives, and leadership. We believe that assimilating these elements of organizational culture will enable organizations to support and sustain innovative activities. Copyright © 2007 John Wiley & Sons, Ltd.
Knowledge transfer across borders: a process model
Authors: Evaristo J. Roberto 
Published: July 2007

AbstractAn expectation‐based process model of knowledge transfer across borders is proposed. The effectiveness of such transfer is postulated to be dependent on the alignment between individual and organizational expectations surrounding the transfer of knowledge. Individual expectations regarding transfer of knowledge are formed through bounded rational cost‐benefit considerations and moderated by culturally and context‐bound expectations. Practical suggestions on how to improve such alignment through specific implementation alternatives are offered. Copyright © 2007 John Wiley & Sons, Ltd.
Organizational culture and knowledge sharing at the United Nations: using an intranet to create a sense of community
Authors: Stoddart Linda 
Published: July 2007

AbstractThis paper describes the impact that the United Nations' (UN) intranet has had on enhancing internal communications in the organization during a period of organizational change, examining in particular the role of a community of content providers. The UN's intranet, called iSeek, was revamped and redesigned in 2005, just before the launch of a major UN reform package. An Internal Communications Unit (ICU) was set up to coordinate a more participatory and dynamic approach to communications and encourage dialog at all levels. A sense of community has been fostered by the creation of a network of focal points providing content across the organization worldwide, which was reinforced during a workshop organized to develop a strategy for the intranet. This community approach has helped encourage knowledge sharing and a transition toward a more collaborative organizational culture. Copyright © 2007 John Wiley & Sons, Ltd.
An analysis of trust among globally distributed work teams in an organizational setting
Authors: Newell Sue , David Gary , Chand Donald 
Published: July 2007

AbstractRegardless of whether a project team is located in the same workplace or distributed around the world, trust remains an important element deemed necessary to facilitate knowledge sharing and collaborative work. At the same time, distribution across sites presents challenges to trust building that are not present among co‐located teams. A further complicating factor in trust building among distributed teams is national culture. As we demonstrate, the impact of nationality can be increased when organizations put the distributed sites in a competitive frame. Using the Newell and Swan threefold typology of trust, this paper analyzes trust among IT work teams whose members are located at sites that are distributed in the United States, Ireland, and India. Our case analysis confirms the problematic nature of trust building among globally distributed teams. Specifically, we found that due to situational factors and socio‐psychological dynamics an ‘Us versus Them’ attitude prevails among the distributed sites. This paper concludes that the traditional approaches used by organizations to address the challenges of global collaboration are insufficient and that trust building in an organizational setting requires project managers to actively work on relationship management to minimize the impact of an inter‐group perspective. Copyright © 2007 John Wiley & Sons, Ltd.
Product line extensions: causes and effects
Authors: Axarloglou Kostas 
Published: July 2007

AbstractIn this paper, and using data from a sample of five US manufacturing industries, we study the implications of market demand growth on product line extensions and the effects of the latter on industry profit margins. Companies extend their product lines in response to expansions in market demand and this tends to depress profit margins in the industry. Finally, these results are quantitatively significant. Copyright © 2007 John Wiley & Sons, Ltd.
Price variability and price dispersion in a stable monetary environment: evidence from German retail markets
Authors: Fengler Matthias R. , Winter Joachim K. 
Published: July 2007

AbstractWe investigate the relationship between inflation and price variation using highly disaggregated, weekly price data for consumption goods recorded in Germany during 1995, a low‐inflation period. We find a significant positive correlation between the rates of price change and price dispersion, both at the level of individual products and product groups. However, we find no correlation between the rates of price change and price variability. Together with results from similar studies, Tommasi (1993. Optimal Pricing, Inflation, and the Cost of Price Adjustment. MIT Press: London, Cambridge, MA; 485–511) and Parsley (1996. J. Money Credit Banking 28: 323–341), a remarkable pattern emerges: when aggregate nominal shocks are small, only price dispersion is correlated with price changes. As the rate of inflation rises, both variability and dispersion become affected. During hyper‐inflation, systematic movements of price dispersion seem to disappear. We conclude that price dispersion is best explained by micro‐economic frictions in price adjustment, whereas price variability appears to be related to costly price search and information problems. Copyright © 2007 John Wiley & Sons, Ltd.
Calculation of pattern in UTD method based on NURBS modeling with the source on surface
Authors: Nan Wang , ChangHong Liang , HaoBo Yuan 
Published: July 2007

AbstractThe UTD method with the source on convex surface which is usually used in the analysis and design of antennas especially conformal arrays on electrically large platforms is studied in this article where NURBS is introduced as way of modeling. Together with the information from ray‐tracing done before, the calculation of fields is presented which includes direct field in the lit region and creeping ray field in the shadow region. The method can be applied to arbitrary electrically large targets and the usefulness can be seen in the examples given. © 2007 Wiley Periodicals, Inc. Microwave Opt Technol Lett 49: 2492–2498, 2007; Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/mop.22727
Continuously tunable incoherent microwave photonic filter using a tunable Mach‒Zehnder interferometer as the slicing filter
Authors: Fu Hongyan , Chen Daru , Ou Haiyan , He Sailing 
Published: July 2007

AbstractA continuously tunable incoherent microwave photonic filter employing a tunable Mach‐Zehnder interferometer (MZI) as the slicing filter is studied. By incorporating the optical variable delay line (OVDL), the frequency spacing of the MZI can be tuned continuously, which in turn makes the whole microwave photonic filter continuously tunable in a wide frequency range. The frequency response characteristics are studied and some simulation results are also given to optimize the present microwave photonic filter. Experimental results show the present filter has good frequency response characteristics and tuning linearity. © 2007 Wiley Periodicals, Inc. Microwave Opt Technol Lett 49: 2382–2386, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.22732