bluuebunny/crossref_metadata_2025_split huggingface dataset:
SPOP attenuates the proliferation, invasion, and migration of choriocarcinoma JAR cells by promoting KIF23 degradation
Authors: Zhou Chunli , Chen Yiyu , Jiang Hairong , Xia Chenchen , Yuan Xiaohan , Yu Qiubo 
Published: April 2024

Abstract

Objectives
Choriocarcinoma is a highly malignant and aggressive trophoblastic tumor. In our previous study, we discovered that the speckle-type POZ protein (SPOP), which recognizes substrates of E3 ubiquitin ligase, plays a crucial role in trophoblast-derived choriocarcinoma cell lines. Therefore, we investigated the correlation between SPOP and the substrate kinesin-like protein KIF23, as well as the role of KIF23 in choriocarcinoma cells.

Methods
We constructed JAR cells overexpressing SPOP using lentiviral vectors and subsequently screened the related proteins through ubiquitination-modified quantitative proteomic analysis. The relationship between KIF23 and SPOP was determined using western blotting, and CCK-8, plate cloning, flow cytometry, and Transwell assays were used to investigate the effects of KIF23 and SPOP/KIF23.

Results
We identified the KIF23 protein and observed that SPOP promoted its degradation. The abundance of KIF23 increased after the addition of the protease inhibitor MG132. KIF23 was highly expressed in choriocarcinoma cells. Compared with JAR cells transfected with NC–small-interfering RNA (siRNA), the proliferation, invasion, migration, and percentage of G0/G1 cells in the KIF23-siRNA group were significantly lower, and the activation of the Akt/GSK3β signaling pathway was markedly attenuated. Additionally, the sh-SPOP+KIF23-siRNA group exhibited significantly inhibited JAR cell proliferation, invasion, and migration, along with clearly attenuated activation of the Akt/GSK3β signaling pathway.

Conclusions
SPOP attenuates the proliferation, invasion, and migration of choriocarcinoma JAR cells by promoting KIF23 degradation.
Impossible homecomings in the Scythian logos of Herodotus
Authors: Sarefield Daniel 
Published: April 2024

AbstractThis paper explores the theme of the homecoming (nostos) by examining the homecomings of the Scythians in Book Four of The Histories of Herodotus from two different approaches, the philological and historical. As Herodotus makes clear, for Scythians, such as the famous traveler Anacharsis and the Scythian king Scyles, returning home could be deadly. From the philological approach, which emphasizes the literary nature of the Scythian logos, this pattern of thematic repetitions of denied homecomings serves to emphasize the hostile nature of Scythia for outsiders and thus to increase the tension surrounding the outcome of the larger narrative of Book Four, which describes the disastrous military campaign of the Persian king Darius I in Scythia. However, from the historical approach, which regards the account of Herodotus as a historical source that provides valuable testimony when combined with other sources of evidence, it becomes clear that these stories of impossible homecomings also reflect the conditions at the Greek frontier of the Scythian world and for Scythians like Anacharsis and Scyles who adopted foreign customs, especially Greek religious practices, namely that in this region marked by competition and conflict, including religious conflict, adopting foreign customs meant it was not possible to return home again.
Unlocking cold-pressed nut potential: focus on tocopherol content of oils and defatted cakes
Authors: Panzanini Marco , Genesi Martina , Zeppa Giuseppe , Dordoni Roberta 
Published: April 2024

The research aimed to survey the composition of oil and partially defatted cakes obtained by cold-pressing various tree nuts to enable their use as ingredients for modulating the nutritional properties of final food products. Different types of pistachios (Pistacia vera), hazelnuts (Corylus avellana), and apricot kernels (Prunus armeniaca) were evaluated in terms of colour, water activity, moisture, ash, fat, protein, fibre, total phenolic, and tocopherol contents. They were extracted by hydraulic presses, giving oils rich in vitamin E and defatted cakes with high nutritional potential: the cakes preserved significant antioxidant compounds, as well as fat, protein, and fibre levels based on the oil extraction yield. Products derived from cold-pressed nuts revealed promising compositions that could be exploited to develop healthy foods with improved sensorial and techno-functional properties, increasing the sustainability of the process and zeroing waste.
Maternal Risk Factors among Pregnant Women with Miscarriage
Authors: Sofia Norlaila , Fadlyana Eddy , Irianti Setyorini , Krisnadi Sofie , Susiarno Hadi 
Published: April 2024

Background: Approximately, 4.1% of maternal deaths in Indonesia occur due to miscarriage. Miscarriage might be caused by various factors such as fetal, maternal, and external factors. Monitoring and early detection of maternal factors might prevent miscarriage. This study aimed to analyze the influence of maternal factors on the incidence of miscarriage. Methods: This was an analytic observational study with a case-control design conducted in Banjar District, South Kalimantan in August–September 2022. Data was collected from the medical records of pregnant women who experienced miscarriage (n=60) and pregnant women who did not (n=120). Data was analyzed using the chi square test and logistic regression. The level of maternal risk factors for the incidence of miscarriage was determined from the odds ratio (OR) value at a 95% confidence interval.Results: The results revealed that parity (p=0.000; OR=2.2), nutritional status (p=0.000; OR=77.1), level of education (p=0.001; OR=3.5), and employment status (p=0.000; OR=8.1) had a significant effect on the incidence of miscarriage.Conclusion: Maternal factors such as parity, nutritional status, education level, and employment status influence on the incidence of miscarriage, therefore, it is essential to educate pregnant women on various factors related to miscarriage.
Clinical Symptoms and Treatment Outcomes of COVID-19 Patients with Stroke
Authors: Daud Oskar Yuda , Sinurat Robert 
Published: April 2024

Background: COVID-19 patients who come to the emergency room often suffer from stroke. This study aimed to examine clinical symptoms and treatment outcomes of COVID-19 patients with stroke.Methods: This study was a retrospective study using secondary data from medical records of COVID-19 patients with stroke, hospitalized at the Universitas Kristen Indonesia Hospital, Jakarta, Indonesia for the period 2020–2022. Patients with PCR test positive for COVID-19 who had clinical symptoms of stroke, confirmed by CT scan showing brain ischemic or hemorrhage were included. Data on gender, age, Glasgow coma scale (GCS), subtype of stroke, severity of COVID-19, and outcome were recorded and analyzed using paired t-test and the Spearman correlation.Results: Of the 49 patients included, 91.8% were over 45 years old and suffered from severe COVID-19. Men were slightly more (51.1%) than women and 93.9% of patients suffered from ischemic stroke. The GCS scores of 46.9% of patients decreased significantly during hospitalization (p=0.02). Most of patients (65.3%) had used non-rebreathing mask (NRM), 10.2% ventilators, and others used nasal cannulas. As many as 46.9% of patients died, 44.9% recovered with neurological deficits, and only 8.2% recovered completely. Interestingly, oxygen delivery devices were significantly correlated with outcome (p=0.00). Patients receiving the antiviral favipiravir had better outcomes compared with those taking remdesivir (p=0.021).Conclusion: The mortality rate for stroke patients with severe COVID-19 is high, and the outcome of patients using NRM or ventilator is worse than patients using nasal cannulas. The antiviral Favipiravir has better outcomes for stroke patients with severe COVID-19.
Implementación de un programa de tenis para optimizar el aprendizaje psicomotriz en niños de 5 a 10 años
Authors: Rojas-Sarmiento Marisol 
Published: April 2024

Los estilos de enseñanza del deporte son diversos y en la actualidad hay distintas teorías que refieren a profundidad sobre su importancia, la enseñanza del tenis asume orientaciones diversas, desde promover la enseñanza basada en el juego hasta el desarrollo de ejercicios de técnica. La investigación surgió de un cuestionamiento sobre el cómo se programa la práctica de tenis en las escuelas de formación deportiva con niños. Su objetivo fue optimizar el aprendizaje psicomotriz en las clases de tenis en niños de 5 a 10 años, mediante la implementación de un programa diseñado para ello. Es una investigación de tipo cualitativa y de corte transversal, en la cual se empleó un test para evaluar el desarrollo psicomotriz para el inicio del programa, y posteriormente una evaluación de control, para determinar cambios en las subcategorías seleccionadas. Aunque existen test e instrumentos para valorar el desarrollo psicomotor, no se identificaron programas de tenis orientados al desarrollo psicomotor. Las variables seleccionadas fueron control corporal, conocimiento de derecha e izquierda, equilibrio dinámico y coordinación óculo manual. Los resultados muestran mejoras significativas en: control corporal del 33,67% (α < 0,025); conocimiento de derecha e izquierda se notó un incremento 126,60% (α < 0,005); equilibrio dinámico se incrementó el nivel en 26,82% (α < 0,1) y en coordinación óculo manual los valores cambiaron para mejor en 44,74% (α < 0,1). En síntesis se ven aportes muy significativos para la enseñanza del tenis en el programa con efectos muy positivos para la mejora continua del aprendizaje psicomotriz.
Stimulus Pertumbuhan Organ Reproduksi Ayam Buras Betina melalui Aplikasi Pemberian Phytoestrogen dari Kelompok Isoflavonoid Hasil Ekstrak Limbah Edamame
Authors: Nurkholis Nurkholis , Nusantoro Suluh , Setiawan Dwi Agus , Andriani Mira , Syahniar Theo Mahiseta 
Published: April 2024

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi pertumbuhan dan perkembangan organ reproduksi ayam buras betina yang diberi flavonoid dari ekstrak limbah kedelai edamame. Penelitian dilakukan dalam dua tahap; kuantifikasi total flavonoid ekstrak limbah kedelai edamame dan eksperimen menggunakan ayam buras berumur 8-16 minggu. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) terdiri atas empat kelompok umur dan lima perlakuan pemberian ekstrak limbah edamame per oral, dengan level 0% (P0), 0,02% (P1), 0,04% (P2), 0,08% (P3), dan 0,16% (P4) dari jumlah pakan yang diberikan. Variabel yang diamati yaitu pertambahan bobot badan (PBB), berat organ reproduksi, dan panjang saluran reproduksi. Data yang diperoleh dianalisis dengan analisis varian dan uji lanjut menggunakan Duncan. Hasil uji kuantitatif menunjukkan bahwa ekstrak limbah edamame mengandung senyawa flavonoid dari golongan isoflavon dan secara kuantitatif diperoleh 40 ppm total flavonoid. Penggunaan ekstrak limbah edamame secara signifikan meningkatkan PBB, panjang saluran reproduksi, dan berat organ (P0,05). (Stimulus for reproductive organs growth of female domestic chickens through phytoestrogens application of isoflavones group from edamame waste extracts) ABSTRACT. This study aimed to access the growth and development of female domesticated chicken reproductive organs after being treated with the addition of flavonoid compound from extract of edamae waste. The research consisted of two experiments; quantification of flavonoids compounds in edamame waste and feeding trial using female domesticated chickens (aged 4-16 weeks). The later used Randomized Block Design, consisted of four groups and five treatments (addition of edamame waste extract at 0%, 0.02%, 0.04%, 0.08%, and 0.16% of the amount of feed given). The variables observed were body weight gain, reproductive organ weight, and reproductive tract length. The data obtained were analyzed using analysis of variance analysis of variance (ANOVA) and Duncan's test. The phytochemical test showed that the edamame waste extract contained flavonoid compounds from the isoflavone group. The addition of edamame waste extract significantly affected body weight gain, reproductive tract length, and reproductive organ weight (P0.05).
Polymorphisms of IGF-1 Gene in Indonesian Local Goat Reared Under Smallholder Farmers in Sulawesi Region
Authors: Rahim Lellah , Dagong Muhammad Ihsan Andi , Bugiwati Sri Rachma Aprilita , Purnomo Nurul 
Published: April 2024

ABSTRACT. The objective of this study was to determine the allele frequency of IGF-1 in Kacang and Peranakan Ettawa goats in Indonesia. The DNA samples were extracted from the blood of 105 heads of goats and collected from the South Sulawesi and West Sulawesi provinces. The IGF-1 target was amplified using the PCR-RFLP method. Two allele variants (A and B) and three genotypes of the IGF-1 gene (AA, AB, and BB) have been identified in the local goat population. Allele frequencies of IGF-1 were A (0.81) and B (0.19) in the total population of local goats. Allele A is the most common allele in both the Kacang and Peranakan Ettawa goat populations, with the highest frequency found in the Kacang population (0.87). The most common genotype is genotype AA, with the highest frequency in the Kacang population (0.75). The observed and expected heterozygosity were 0.276 and 0.303, respectively. The population of local goats in these regions was in Hardy-Weinberg equilibrium. The conclusion of this research is that the IGF-1 gene in local goats in the Sulawesi region is polymorphic and this diversity information can be used for association studies with growth traits, litter size, and twinning rate. (Polimorpisme gen IGF-1 pada populasi kambing lokal Indonesia yang dipelihara oleh peternak kecil di daerah Sulawesi) ABSTRAK. Penelitian ini bertujuan untuk mengetahui frekuensi alel IGF-1 pada kambing Kacang dan Peranakan Ettawa di Indonesia. Sampel DNA diambil dari darah 105 ekor kambing dan dikumpulkan dari Provinsi Sulawesi Selatan dan Sulawesi Barat. Target IGF-1 diamplifikasi menggunakan metode PCR-RFLP. Dua varian alel (A dan B) dan tiga genotipe gen IGF-1 (AA, AB, dan BB) telah diidentifikasi pada populasi kambing lokal. Frekuensi alel IGF-1 adalah A (0,81) dan B (0,19) pada total populasi kambing lokal. Alel A merupakan alel yang paling banyak ditemukan baik pada populasi kambing Kacang maupun kambing Peranakan Ettawa, dengan frekuensi tertinggi terdapat pada populasi Kacang (0,87). Genotipe yang paling umum adalah genotipe AA, dengan frekuensi tertinggi pada populasi Kacang (0,75). Heterozigositas yang diamati dan diharapkan adalah 0,276 dan 0,303, masing-masing. Populasi kambing lokal di wilayah ini berada dalam keseimbangan Hardy-Weinberg. Kesimpulan yang dihasilkan adalah bahwa gen IGF-1 pada kambing lokal yang dipelihara oleh peternak di wilayah Sulawesi bersifat polimorfik dan informasi keragaman ini dapat digunakan untuk studi asosiasi dengan sifat pertumbuhan, litter size dan kelahiran kembar.
Kualitas Spermatozoa dan Tudung Akrosom Utuh pada Semen Beku Sapi Friesian Holstein dengan Mutu Genetik yang Berbeda
Authors: Yekti Aulia Puspita Anugra , Umamah Azna Roudlotul Nur , Safa Firlia , Andriani Nadya Meyta , Febrianto Nanang , Susilawati Trinil 
Published: April 2024

ABSTRAK. Penelitian ini bertujuan untuk mengetahui analisis kualitas spermatozoa dan tudung akrosom utuh semen beku pada pejantan sapi Friesian Holstein (FH) dengan mutu genetik yang berbeda. Metode penelitian yang digunakan adalah penelitian observasional dengan 2 kelompok perlakuan jenis semen beku yang berbeda. Kelompok perlakuan jenis semen beku adalah Sapi FH proven bull (P1) dan sapi FH grade B (P2). Parameter yang diamati meliputi motilitas spermatozoa, konsentrasi spermatozoa, viabilitas spermatozoa, abnormalitas spermatozoa, dan tudung akrosom utuh (TAU) spermatozoa. Data dianalisis menggunakan uji-t untuk mengetahui perbedaan kualitas antar kelompok perlakuan, kemudian dilakukan uji chi-square untuk membandingkan nilai hasil observasi dengan nilai harapan. Hasil penelitian menunjukkan bahwa kualitas semen beku pada sapi FH proven bull dan sapi FH grade B tidak berbeda nyata (P0,05). Disimpulkan bahwa perbedaan mutu genetik pada pejantan tidak memengaruhi kualitas spermatozoa semen beku sapi FH. (Sperm quality and intact acrosome cap of Friesian Holstein frozen semen with different genetic qualities) ABSTRACT. This study aimed to determine the quality of spermatozoa and intact acrosome cap of Friesian Holstein bulls with different genetic qualities. This study used observational research with two categories of frozen semen types treatment. The frozen semen consisted of Friesian Holstein Proven Bull (T1) and Friesian Holstein grade B bull (T2). The parameters observed include sperm motility, sperm concentration, sperm viability, sperm abnormality, and intact acrosome cap. Data were analyzed using a t-test to determine the differences in quality between treatments. Then, the chi-square test was used to compare the observed and expected values. The results showed no significant difference (P0.05) in frozen semen quality of Friesian Holstein proven bull and Friesian Holstein grade B bull. In conclusion, the different genetic qualities of bulls did not affect the quality of frozen semen.
Efektivitas Amoniasi, Fermentasi, dan Amoniasi Fermentasi dengan Trichoderma harzianum pada Jerami Sereh Wangi (Cymbopogon nardus)
Authors: Solehudin Solehudin , Hernaman Iman , Ayuningsih Budi , Pamungkas Dicky 
Published: April 2024

ABSTRAK. Penelitian ini bertujuan untuk mengetahui pengaruh ransum yang mengandung azolla (Azolla microphylla) terhadap indeks kuning telur, indeks putih telur dan haugh unit telur ayam. Azolla merupakan tanaman paku air yang pertumbuhan cepat dan dapat dijadikan bahan pakan alternatif untuk ayam petelur. Penelitian menggunakan 48 ekor ayam petelur strain ISA Brown berumur 54 minggu di Fakultas Peternakan, Universitas Padjadjaran. Penelitian ini dilakukan secara eksperimental dengan metode Rancangan Acak Lengkap (RAL) dengan 4 perlakuan (P0= ransum tanpa azolla; P1= ransum dengan 5% azolla; P2= ransum dengan 10% azolla; dan P3= ransum dengan 15% azolla) dan 6 ulangan. Hasil penelitian menunjukkan bahwa ransum yang mengandung azolla berpengaruh tidak nyata (P0,05) terhadap indeks putih telur, indeks kuning telur dan haugh unit telur. Penggunaan azolla sampai taraf 15% menghasilkan nilai yang sama terhadap indeks kuning telur, indeks putih telur dan haugh unit telur ayam. (Effectiveness ammoniation, fermentation and amoniated fermentation with Trichoderma harzianum in citronella straw (Cymbopogon nardus)) ABSTRACT. The research was conducted to investigate effectiveness of ammoniation, fermentation and ammoniated fermentation with Trichoderma harzianum on citronella (Cymbopogon nardus) straw. The research used Completely Randomized Design (CRD) with 3 treatments: citronella straw with ammoniation treatment (P1), citronella straw with fermentation treatment (P2), and citronella straw with ammoniated fermentation treatment (P3). Each treatment was replicated five times. The parameters measured were an increase in crude protein content, an increase in Total Digestible Nutrient (TDN), a decrease in crude fiber, and a decrease in lignin. Variance analysis was conducted to determine treatment effect and followed by Multiple Range of Duncan to determine difference between treatment. The result showed that treatments give significant effect (P0.05) on increasing of crude protein, increasing of TDN, decreasing of crude fiber and decreasing of lignin. The best value processing of citronella straw was ammoniated fermentation with Trichoderma harzianum. It concluded that ammoniated fermentation with Trichoderma harzianum had the best effectiveness in processing of citronella straw.
Pengaruh Serbuk Teh Hijau Sebagai Pakan Aditif terhadap Performa Domba Betina yang Diinfeksi Strongyles
Authors: Utama Aldyansah Putra , Mayasari Novi , Yuniarti Endah , Ramdani Diky 
Published: April 2024

ABSTRAK. Serbuk teh hijau merupakan salah satu bahan aditif yang dapat ditambahkan ke dalam pakan ternak yang dapat berfungsi sebagai anthelmintik dalam mengurangi infeksi cacing dan meningkatkan kualitas pakan bagi ternak. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian berbagai dosis serbuk teh hijau terhadap konsumsi pakan dan pertambahan bobot badan domba betina yang diinfeksi Strongyles Penelitian dilaksanakan di Laboratorium Lapangan Ternak Domba dan Kesehatan Hewan, Jurusan Peternakan, Polbangtan, Bogor pada bulan Agustus-Oktober 2021. Penelitian menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) yang terdiri dari tiga perlakuan tingkat pemberian serbuk teh hijau, yaitu P0= 0%, P1= 0,75%, P2= 1,5% dari bahan kering ransum, dengan enam ulangan untuk setiap perlakuannya sehingga total ternak adalah 18 ekor. Data hasil penelitian dianalisis menggunakan analisis ragam. Parameter yang diukur meliputi konsumsi pakan, pertambahan bobot badan, dan ukuran-ukuran tubuh ternak. Hasil penelitian menunjukkan bahwa pemberian serbuk teh hijau 1,5% (P2) dalam pakan domba betina tidak berpengaruh nyata terhadap konsumsi bahan kering, tinggi pundak, tinggi pinggang, lebar pinggul, pertambahan bobot badan, panjang badan, lingkar dada, dan lebar dada domba yang diinfeksi, namun dapat menekan penurunan bobot badan yang diakibatkan oleh parasit cacing. (The effect of green tea dust as a feed additive on performance ewes infected by strongyles) ABSTRACT. Green tea dust is one of feed additives that can be added to animal diet which can function as an anthelmintic in reducing worm infections and improving the performance of the livestock. This study aimed to determine the effect of various doses of green tea dust supplementation on dry matter intake (DMI, g/kg DM), average daily gain (ADG, g/kg DM), and body measurements(cm) of ewes infected with Strongyles. The research was conducted at the Sheep Farm and Animal Health Laboratory, Department of Animal Husbandry, Polbangtan, Bogor in August-October 2021. A completely randomized design was used as the experimental method consisted of three treatments of different levels of green tea dust supplementation, namely P0= 0%, P1= 0.75%, P2= 1.5% from dry matter ration, using 6 replicatess to obtain a total of 18 experimental units. The results showed that the administration of 1.5% green tea dust (P2) had no significant effect on DMI, ADG, and body measurements such as shoulder height, waist height, hip width, body length, chest circumference, and chest width of sheep, which were infected with Strongyles, but it tended to reduce their body weight losses.