bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intraoperative indocyanine green fluorescence imaging to predict early hepatic arterial complications after liver transplantation
Authors: Terasawa Muga , Imamura Hiroshi , Allard Marc Antoine , Pietrasz Daniel , Ciacio Oriana , Pittau Gabriella , Salloum Chady , Sa Cunha Antonio , Cherqui Daniel , Adam René , Azoulay Daniel , Saiura Akio , Vibert Eric , Golse Nicolas 
Published: March 2024

The purpose of this study was to propose an innovative intraoperative criterion in a liver transplantation setting that would judge arterial flow abnormality that may lead to early hepatic arterial occlusion, that is, thrombosis or stenosis, when left untreated and to carry out reanastomosis. After liver graft implantation, and after ensuring that there is no abnormality on the Doppler ultrasound (qualitative and quantitative assessment), we intraoperatively injected indocyanine green dye (0.01 mg/Kg), and we quantified the fluorescence signal at the graft pedicle using ImageJ software. From the obtained images of 89 adult patients transplanted in our center between September 2017 and April 2019, we constructed fluorescence intensity curves of the hepatic arterial signal and examined their relationship with the occurrence of early hepatic arterial occlusion (thrombosis or stenosis). Early hepatic arterial occlusion occurred in 7 patients (7.8%), including 3 thrombosis and 4 stenosis. Among various parameters of the flow intensity curve analyzed, the ratio of peak to plateau fluorescence intensity and the jagged wave pattern at the plateau phase were closely associated with this dreaded event. By combining the ratio of peak to plateau at 0.275 and a jagged wave, we best predicted the occurrence of early hepatic arterial occlusion and thrombosis, with sensitivity/specificity of 0.86/0.98 and 1.00/0.94, respectively. Through a simple composite parameter, the indocyanine green fluorescence imaging system is an additional and promising intraoperative modality for identifying recipients of transplant at high risk of developing early hepatic arterial occlusion. This tool could assist the surgeon in the decision to redo the anastomosis despite normal Doppler ultrasonography.
Reactive axillary lymph nodes after COVID-19 mRNA vaccination: comparison of mRNA vs. attenuated whole-virus vaccines
Authors: Adin Mehmet Emin , Isufi Edvin , Wu Jennifer , Pang Yulei , Nguyen Daniel , Simsek Has Duygu , Caner Civan , Aboueldaha Noha , Mossa-Basha Mahmud , Pucar Darko 
Published: March 2024

Objective
To compare the incidence and natural course of reactive axillary lymph nodes (RAL) between mRNA and attenuated whole-virus vaccines using Deauville criteria.

Methods
In this multi-institutional PET-CT study comprising multiple vaccine types (Pfizer–BioNTech/Comirnaty, Moderna/Spikevax, Sinovac/CoronaVac and Janssen vaccines), we evaluated the incidence and natural course of RAL in a large cohort of oncological patients utilizing a standardized Deauville scaling system (n=522; 293 Female, Deauville 3-5 positive for RAL). Univariate and multivariate analyses were conducted to evaluate the predictive value of clinical parameters (absolute neutrophil count [ANC], platelets, age, sex, tumor type, and vaccine-to-PET interval) for PET positivity.

Results
Pfizer–BioNTech/Comirnaty and Moderna vaccines revealed similar RAL incidences for the first 20 days after the second dose of vaccine administration (44% for the first 10 days for both groups, 26% vs. 20% for 10–20 days, respectively for Moderna and Pfizer). However, Moderna recipients revealed significantly higher incidences of RAL after 20 days compared to Pfizer–BioNTech/Comirnaty, with nodal reactivity spanning up to the 9th week post-vaccination (15% vs. 4%, respectively P < 0.001). No RAL was observed in patients who received either a single dose of J&J vaccine or two doses of CroronaVac. Younger patients showed increased likelihood of RAL, otherwise, clinical/demographic parameters were not predictive of RAL (P = 0.014 for age, P > 0.05 for additional clinical/demographic parameters).

Conclusion
RAL based on strict PET criteria was observed with mRNA but not with attenuated whole-virus vaccines, in line with higher immunogenicity and stronger protection offered by mRNA vaccines.
Respiratory adenovirus infections in children: a focus on Africa
Authors: van der Zalm Marieke M. , Sam-Agudu Nadia A. , Verhagen Lilly M. 
Published: March 2024

Purpose of review
Lower respiratory tract infections (LRTIs) are an important cause of child morbidity and mortality globally, especially in children under the age of 5 years in Africa. Respiratory viruses, including human adenoviruses (HAdVs), are common causes of LRTIs in children. This review aims to shed light on the epidemiology, clinical manifestations, sequelae, and treatment options specific to adenovirus respiratory infections in African children.

Recent findings
Recent evidence has challenged the perception that adenovirus is a negligible cause of LRTIs. Studies show HAdV emerging as the third most common viral pathogen in fatal pneumonias among under-5 children in low-income and middle-income African countries, contributing to 5.5% of all pneumonia deaths and ranking second in hospital-associated viral pneumonia deaths. Predominant HAdV serotypes associated with disease differ by country and region, and have changed over time. Risk factors for increased disease severity and long-term respiratory sequelae in previously healthy African children with HAdV LRTIs are not well established.

Summary
Although respiratory viruses, including HAdV, are recognized contributors to LRTIs, the prevalence and impact of adenovirus infections have been under-recognized and understated. Available data suggests that African children, particularly those under 5 years old, are at risk of severe sequelae from respiratory HAdV infections. Long-term sequelae, including bronchiectasis and postinfectious bronchiolitis obliterans, further underscore the significant impact of HAdV infections. However, the scarcity of comprehensive data hampers our understanding of the extent of the impact of HAdV infections on child lung health in Africa. We recommend scaled-up HAdV surveillance, ensuring its consistent inclusion in population-level LRTI assessments, and expanded and equitable access to diagnostics for early recognition of African children at risk of developing chronic sequelae and death. Enhanced understanding of adenovirus epidemiology and clinical outcomes and the availability of therapeutic options are essential for informed public health strategies and clinical care.
Effective Treatment of Lymphogranuloma Venereum With a 7-Day Course of Doxycycline
Authors: Bilinska Julia , Artykov Ruslan , White John 
Published: March 2024

Background
Lymphogranuloma venereum (LGV) remains endemic in the United Kingdom, primarily among gay, bisexual or other men who have sex with men (GBMSM). Current treatment guidelines recommend 21 days of doxycycline, but recent evidence suggests shorter antibiotic duration is as effective. We evaluated clinical outcomes in a cohort with LGV treated with 7 days of doxycycline.

Methods
We reviewed case notes of all LGV cases at a South London sexual health service between November 2016 and September 2022, treated with only 7 days of doxycycline and anonymized data were collected from electronic patient records.

Results
Fifty-two individuals with detected LGV-specific DNA were treated with 7 days of doxycycline 100 mg twice daily. All were GBMSM, median age of 35 years (range, 21–64 years), 21 (40%) were living with HIV, and 18 (35%) had concomitant sexually transmitted infections. Thirty-four (65%) were asymptomatic, whereas 18 (35%) reported symptoms: 7 (13%) urethral, 11 (21%) anorectal, and 2 (4%) other symptoms. Twenty-two (42%) were prescribed additional antimicrobials; however, none were active against Chlamydia trachomatis. All 52 underwent follow-up testing (range, 4–481 days). Chlamydia trachomatis was detected in one individual, but negative for LGV-specific DNA, and so considered to be a reinfection. All other cases were C. trachomatis-negative, indicating successful LGV eradication.

Conclusions
Our data support the approach of offering a 7-day doxycycline course routinely for asymptomatic or clinically mild C. trachomatis infections, and contacts of LGV infection, regardless of their LGV status. This may simplify patient management, reduce cost, and improve antimicrobial stewardship.
Aromatase Inhibitor-Associated Distal Radioulnar Joint Instability and Tear of the Extensor Digiti Minimi: A Case Report
Authors: Holt Jonathan , Salas Michael , Lee Se Won 
Published: March 2024

AbstractThe addition of aromatase inhibitors (AI) has improved cancer-related outcomes in post-menopausal patients with estrogen receptor-positive breast cancer. However, AI can be associated with a constellation of adverse musculoskeletal effects that comprises bone loss, arthralgia, myalgia, and tendinopathy. This medication complication, known as aromatase inhibitor-associated musculoskeletal syndrome (AIMSS), can limit treatment tolerability in many patients due to the high prevalence of AIMSS among those on AI. The hand and wrist are the most affected joints in AIMSS, with patients presenting with symmetric arthralgia, stiffness, and tendinopathy. Radioulnar joint subluxation with extensor tendon tear has not been previously reported in patients with AIMSS. This is a case report of a 72-year-old breast cancer survivor on an AI presenting with chronic dominant wrist pain, weakness, and 5thdigit finger drop. An extensor digitorum minimi tendon tear and radioulnar instability were identified using diagnostic musculoskeletal ultrasonography. This case illustrates the utility of in-office ultrasonography combined with dynamic examination for the often underrecognized pathology associated with AIMSS in breast cancer survivors.
Clinical features and outcomes of mpox in people with and without HIV: a national comparative study
Authors: Núñez Isaac , Ceballos-Liceaga Santa E. , Torre Alethse de la , García-Rodríguez Gabriel , López-Martínez Irma , López-Gatell Hugo , Mosqueda-Gómez Juan L. , Valdés-Ferrer Sergio Iván 
Published: March 2024

Background:
People who live with HIV (PWLH) have been one of the most affected groups during the current mpox outbreak. They are hypothesized to have a more severe clinical course than people without HIV but comparative data is scarce. We aimed to compare clinical features and outcomes of mpox in people with and without HIV in Mexico.

Setting:
Country-wide study in Mexico.

Methods:
We performed an observational study using nation-wide epidemiological data. We included all people with confirmed mpox diagnosed between May and November 2022 in Mexico. Clinical and sociodemographic characteristics were compared between people with and without HIV. Multivariable logistic regression models were preformed to determine the association between HIV, clinical features, and outcomes and reported with odds ratios (ORs) and 95% confidence intervals (95% CI). ORs for rare outcomes were interpreted as risk ratios.

Results:
Among 3291 people with mpox, 59% were PWLH. PWLH had an increased risk of severe mpox (OR 2.6, 2.4-2.9) and death (OR 10.8, 9.7-11.9). They also had a higher risk of otalgia, proctitis, and urethritis. Eleven individuals died, of whom ten were PWLH. All deaths were directly attributed to mpox.

Conclusion:
People with HIV have a higher risk of severe mpox and death due to mpox.
Increased 68Ga-FAPI Uptake in Capillary Hemangiomas in an Adult Patient
Authors: Deng Jia , Zhang Dongmei , Yang Jian , Peng Dengsai , Chen Yue 
Published: March 2024

Abstract
A 67-year-old woman was enrolled in our 68Ga-FAPI PET/CT tumor clinical trial due to her lung adenocarcinoma. The PET/CT scan additionally revealed increased uptake of FAPI in the tongue. Combined with the patient’s medical history and the contrast-enhanced CT of the maxillofacial region, it was suspected to be a hemangioma. Subsequently, the patient underwent surgery and was diagnosed with capillary hemangioma of the left side of her tongue.
Nonmalignant 68Ga-FAPI-46 Uptake in 2 Cases of TENIS Syndrome
Authors: Karamian Farivash , Sadeghi Ramin , Aghaee Atena , Askari Emran , Roustaei Hessamoddin 
Published: March 2024

AbstractThe cancer specificity of fibroblast activation protein inhibitor (FAPI) uptake remains understudied topic, and several cases of false-positive FAPI PET/CT findings have been reported. We present 2 patients of differentiated thyroid cancer with thyroglobulin elevation and negative iodine scintigraphy syndrome who underwent68Ga-FAPI PET/CT and18F-FDG PET/CT for evaluation of any possible sites for metastatic disease. Although no focus of metastatic disease was found in these patients, remarkable findings were noticed instead. Nonmalignant FAPI uptake was evident in the gallbladder, uterus, and degenerative changes, whereas these uptake were discordant or partially concordant with those in FDG’s, also CT images showed no underlying abnormality.
Earlier finish of motor planning in the premotor cortex predicts faster motor command in the primary motor cortex: human intracranial EEG evidence
Authors: Xia Jing , Gong Deshan , Han Biao , Guo Qiang , Fink Gereon R. , Daun Silvia , Chen Qi 
Published: March 2024

AbstractThe human motor system has a hierarchical control during finger movements. The non-primary motor cortex (premotor cortex, PM, and supplementary motor area, SMA) organizes motor planning, while the primary motor cortex (M1) is responsible for motor execution. We utilized the human intracranial EEG’s high temporal and spatial resolution to investigate how the temporal dynamics of the high-gamma neural oscillations in the hierarchically organized motor sub-regions, during both pre-movement planning and motor execution, correlated with reaction times (RTs) in a cued finger movement task. Our results showed that the high-gamma power of PM, SMA, and M1 activated sequentially. More importantly, the sustained high-gamma power activation in the non-primary motor cortex and the peak latency of high-gamma power in M1 had a significant predictive relationship with the RTs. In particular, the faster the activation of the non-primary motor cortex returned to baseline, the faster the motor command in M1, and accordingly the shorter the RTs. Further, pairwise phase coherence between the motor areas showed that the more sustained the connection between the motor areas, the longer the RTs would be. The current findings illustrate the relationship between the temporal profiles of high-gamma power in human motor areas and response performance.
MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition
Authors: Molina David , Horvath Sara , Zhang Xudong , Xiao Wei , Ragab Noah , Ripper Dagmar , Kilian Joachim , Andersen Tonni Grube , Ragni Laura 
Published: March 2024

AbstractPlants have developed specialized barriers to protect and isolate the inner tissues from the environment while maintaining homeostasis. Different barriers are present in various organs and at different growth stages. During secondary growth, the periderm acts as the protective tissue, covering roots, stems, and branches as they become thick. The periderm is a dynamic barrier comprising a stem cell niche known as the cork cambium, which bifacially divides to generate the phelloderm inward and the cork outward. Cork cells have a unique cell wall impregnated with suberin and lignin polymers, essential for the barrier function.Despite its importance, the differentiation process that forms new cork cells from the stem cell is largely unknown. In this work, we identify members of the MYB36-subclade transcription factors as key regulators of cork differentiation. On the one hand, this set of transcription factors promotes suberin deposition by inducing the expression of enzymes involved in all steps of suberin biosynthesis, including the recently discovered suberin-polymerizing enzymes GDS Lipases; on the other hand, it represses cork cambium proliferation. Furthermore, we demonstrated that suberin deposition in the cork is a robust process regulated by a complex network of transcription factors, including other MYB transcription factors that activate suberin deposition in the endodermis. However, only members of the MYB36 subclade can repress cell proliferation in different developmental contexts, highlighting general and specific functions for MYB transcription factors. These findings have broad applicability, as tissue-specific manipulation of MYB activity has the potential for improving traits of biotechnological interest, such as thicker periderms and more suberized cork layers, and for assessing how these traits affect plant performance in response to stresses.
SAMson: an automated brain extraction tool for rodents using SAM
Authors: Panadero Soler Daniel , Kotb Selim Mohamed , Martínez-Tazo Patricia , Muñoz-Moreno Emma , Ramos-Cabrer Pedro , López-Larrubia Pilar , De Santis Silvia , Canals Santiago , Pertusa Antonio 
Published: March 2024

AbstractAccurate brain extraction is a critical step in the analysis of rodent head magnetic resonance imaging (MRI) data. However, current methods often encounter difficulties in handling the diverse range of imaging setups, resolutions, and experimental conditions that are commonly found in this field. Based on the Segment Anything Model (SAM), we introduce here SAMson (SAM for Segmentation Of Neuroimages), an automated tool for robust rodent brain extraction. SAMson integrates a bounding box generator and a mask prediction pipeline, offering fully automated and semi-automated modes to address varying experimental complexities. The performance of SAMson was evaluated using three multi-centre rodent MRI datasets annotated at the pixel level, which differed in terms of acquisition parameters, resolution, and animal age groups. SAMson demonstrates superior performance to existing methods, including BET, RBM, and BEN, in terms of segmentation accuracy, with Jaccard indices exceeding 90% across datasets. The semi-automated mode demonstrates particular efficacy in challenging scenarios, including low-resolution images and cases requiring refined mask precision. In contrast to conventional volumetric techniques, SAMson identifies errors at the level of individual slices, thereby enabling rapid and targeted correction when needed. By providing open-source access, SAMson aims to support large-scale research workflows and advance translational neuroscience. The curated data can be downloaded fromhttps://doi.org/10.20350/digitalCSIC/17000, and the code is available athttps://github.com/CanalsLab/SAMson.
SARS-CoV-2 Genetic Variants and Patient Factors Associated with Hospitalization Risk
Authors: Korves Tonia , Stein David , Walburger David , Adamusiak Tomasz , Roberts Seth 
Published: March 2024

ABSTRACTVariants of SARS-CoV-2 have been associated with different transmissibilities and disease severities. The present study examines SARS-CoV-2 genetic variants and their relationship to risk for hospitalization, using data from 12,538 patients from a large, multisite observational cohort study. The association of viral genomic variants and hospitalization is examined with clinical covariates, including COVID-19 vaccination status, outpatient monoclonal antibody treatment status, and underlying risk for poor clinical outcome. Modeling approaches include XGBoost with SHapley Additive exPlanations (SHAP) analysis and generalized linear mixed models. The results indicate that several SARS-CoV-2 lineages are associated with increased hospitalization risk, including B.1.1.7, AY.44, and AY.54. As found in prior studies, Omicron is associated with lower hospitalization risk compared to prior WHO variants. In addition, the results suggest that variants at specific amino acid locations, including locations within Spike protein N-terminal domain and in non-structural protein 14, are associated with hospitalization risk.
La Crosse virus reassortants highlight genomic determinants of infection and pathogenesis
Authors: Rondeau Nicole C. , Spector Sophie N. , Thannickal Sara A. , Stapleford Kenneth A. 
Published: March 2024

AbstractThe genomic determinants that contribute to orthobunyavirus infection and pathogenesis are not well-defined. In this study, we harnessed the process of reassortment to understand which viral factors drive change in the replication and pathogenesis of La Crosse virus (LACV). We systematically reassorted the genomic segments of two genetically similar Lineage I LACV isolates into six unique reassortants. Despite the parental isolates having high levels of RNA and protein consensus, the reassortants demonstrate how minimal changes in RNA and protein structure can have significant changes in viral growth and reproductionin vitroin mammalian and insect models. We observed that swapping the S segment between isolates led to differences in replication and assembly resulting in one non-rescuable reassortant and one viable reassortant that exhibited an increase in viral growth dynamics. Switching the M segment led to changes in viral plaque phenotype and growth kinetics. L segment reassortants similarly differed in changes in viral growth dynamics. We further explored the M segment reassortants in a neonate mouse model and observed a role for the M segment in neuroinflammation and virulence. Through reassortment of the La Crosse virus genomic segments, we are able to further understand how genomic determinants of infection and pathogenesis operate in orthobunyaviruses. Future investigations will focus on identifying the specific molecular elements that govern the observed phenotypesin vitroandin vivo.ImportanceLa Crosse virus is the leading cause of pediatric arboviral encephalitis in the United States, yet it is largely unknown how each of the three genomic segments contribute to pathogenesis and disease. Our study utilizes genomic reassortment between two similar Lineage I LACV isolates to understand genomic determinants for differences in infection and pathogenesis phenotypes in vitro and in vivo. By identifying roles for each segment in observed outcomes, we are able to plan further studies for molecular characterization of these phenotypes. Additionally, it is imperative to continue to characterize orthobunyavirus function since climate change will expand the range and prevalence of arthropod-borne diseases such as LACV in the United States.
Reanalysis of rare radiative
Ξb−→Ξ−γ
decay in QCD
Authors: Aliev T. M. , Ozpineci A. , Sarac Y. 
Published: March 2024

The upper limit of the branching ratio of the rare Ξb−→Ξ−γ decay is obtained as BR(Ξb−→Ξ−γ)<1.3×10−4 by the LHCb. In the present work we study this decay within the light cone QCD sum rules employing the Ξb distribution amplitudes. At the first stage, the form factors entering the Ξb−→Ξ−γ decay are obtained. Next, using the results for the form factors the corresponding branching ratio for this decay is estimated to be BR(Ξb−→Ξ−γ)=(4.8±1.3)×10−5. This value lies below the upper limit established by the LHCb collaboration. Our finding for the branching ratio is also compared with the results of the other theoretical approaches existing in the literature.

Published by the American Physical Society
2024