bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of citrulline production from a Bacillus subtilis BH-01 isolated from raw buffalo milk
Authors: Mansour Marwa A. K. , Ali Salah G. , Hassan Manal A. M. , Gabra Fify A. , Mawad Asmaa M. M. 
Published: February 2025

Abstract
The main purpose of this study was to optimize the L-citrulline production process using Plackett-Burman and Box-Behnken designs. L-citrulline-producing bacterium BH-01 was isolated from raw buffalo milk. The isolate was tested for probiotic activities such as tolerance to simulated gastric and intestinal juices, antagonistic activity against six antibiotic-resistant bacteria, and temperature tolerance. L-citrulline production and arginine deiminase (ADI) activity were optimized using statistical designs. The bacterial isolate was molecularly identified as Bacillus subtilis strain AUMC B-498 (accession number PP574248.1). The strain exhibited resistance at pH 2.0 and bile salt 0.5% for a two-hour exposure period. It could inhibit the growth of Escherichia coli, Klebsiella pneumonia, Serratia sp., Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and Streptococcus pneumoniae. From the results of statistical optimization, the Plackett-Burman design identified temperature, L-arginine, incubation period, and peptone as the most effective factors among the eight selected variables. Based on these, the Box-Behnken design was used to optimize the factors required to maximize citrulline production. The maximum L-citrulline was 632.5 µg/L, and ADI activity was 1.42 U/mL. Therefore, BH-01 isolated from Buffalo milk might be a promising candidate in food, biotechnological, and pharmaceutical applications due to its dual functionality for citrulline production and probiotic characteristics.
Radiotherapy plus a self-gelation powder encapsulating tRF5-GlyGCC inhibitor potentiates natural kill cell immunity to prevent hepatocellular carcinoma recurrence
Authors: Gong Yihang , Zeng Fanxin , Zhang Feng , Liu Xiaoquan , Li Zhongheng , Chen Wenjie , Liu Haipeng , Li Xin , Cheng Yusheng , Zhang Jian , Feng Yeqian , Wu Tiangen , Zhou Wence , Zhang Tong 
Published: February 2025

Abstract
Hepatocellular carcinoma (HCC) recurrence postresection represents a thorny problem in clinical practice, of which impaired natural killer (NK) cell cytotoxicity represents one of crucial causes. Apart from recurrence, hepatectomy-induced abdominal adhesion also poses huge clinical challenges such as abdominal pain, intestinal obstruction, and perforation. Evidence demonstrates that radiotherapy can upregulate NK group 2D ligand expression on tumor cells to enhance NK cell cytotoxicity, indicating its great potential of curbing HCC recurrence. Nevertheless, radiotherapy has also been disclosed to incur suppression on NK antitumor cell immunity. Herein, we reveal that glycocholic acid (GCA)/tRNA-derived fragment 5 (tRF5)-GlyGCC signaling axis is activated in mouse HCC model after radiotherapy, which dampens NK cell antitumor immunity to limit therapeutic efficacy. Mechanistically, tRF5-GlyGCC can interact with KDM6B to epigenetically upregulate Runx2 and then transcriptionally activate ITGBL1 and S100A9 expression in HCC cells, which further reduces NK cell cytotoxicity directly and attracts myeloid-derived suppressor cell (MDSC) to inhibit NK cell function indirectly, respectively. Therefore, radiotherapy plus targeting tRF5-GlyGCC may be an optimized postoperative adjuvant therapy against HCC recurrence. Then, a nanocomposite powder is designed for liver-localized delivery of tRF5-GlyGCC inhibitor. After sprayed to liver resection margin of mouse HCC model, this powder can rapidly form an in-situ Janus-adhesive hydrogel, which allows for sustained delivery of tRF5-GlyGCC inhibitor. Importantly, it can synergize with radiotherapy to potentiate NK cell antitumor immunity and prevent HCC recurrence postresection. Moreover, its application to surgical bed also effectively mitigates abdominal adhesion in a rat hepatectomy model. Altogether, our work develops a tRF5-GlyGCC-targeting nanocomposite power for sensitizing radiotherapy to thwart HCC recurrence and preventing abdominal adhesion.

Graphical Abstract
Long-read sequencing and genome assembly of natural history collection samples and challenging specimens
Authors: Bein Bernhard , Chrysostomakis Ioannis , Arantes Larissa S. , Brown Tom , Gerheim Charlotte , Schell Tilman , Schneider Clément , Leushkin Evgeny , Chen Zeyuan , Sigwart Julia , Gonzalez Vanessa , Wong Nur Leena W. S. , Santos Fabricio R. , Blom Mozes P. K. , Mayer Frieder , Mazzoni Camila J. , Böhne Astrid , Winkler Sylke , Greve Carola , Hiller Michael 
Published: February 2025

Abstract
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assemble the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit. Our protocol also improves assemblies of other difficult-to-sequence molluscs and arthropods, including millimeter-sized organisms. By highlighting collections as valuable sample resources and facilitating genome assembly of tiny and challenging organisms, our study advances efforts to obtain reference genomes of all eukaryotes.
Illustrating Fuego: the particular challenges and richness of using arts-based participatory methods to communicate experiences of volcanic disaster
Authors: Naismith Ailsa K. 
Published: February 2025

Abstract
Although disasters disproportionately affect vulnerable people, their experiences often go untold. Disaster researchers increasingly use participatory research methods to recognize and amplify these missing voices. Arts-based participatory methods promise both empowerment for local people and opportunity for researchers to work creatively and reflexively. They also present challenges of participation and representation. This paper describes the process of co-producing a “zine” describing local people’s experiences of eruptions of Fuego volcano (Guatemala) and the impacts of these eruptions that caused disaster. The zine is narrated by local people and is illustrated by the researcher (me) who conducted semi-structured interviews of residents to better understand their experiences. The motivations for this project are explored together with lessons learned in turning participatory research design into practice. Taking a constructively critical perspective on my process, I document challenges I encountered in community participation and representation and discuss how they may be addressed in project design and execution. I also describe the tensions and value in occupying both researcher and artist roles. This paper describes an open method of research exploration where the opportunities and limitations of visual representation to contribute to shared and widening understandings of volcanic disaster experience is documented and reflected upon.
Behavioral, neuronal, and physiological facets of multidimensional body image in anorexia nervosa: a scoping review
Authors: Di Giuliano Monica , de la Cruz Feliberto , Schumann Andy , Zopf Regine , Bär Karl-Jürgen 
Published: February 2025

Abstract
Body image is a core identity facet in anorexia nervosa (AN). Current literature outlines body image as a multidimensional construct, characterized by perceptual, cognitive, and emotional components, which explain how individuals perceive, think, and behave in relation to their body. The purpose of this scoping review is to uncover neuropsychological disturbances in AN related to body image by focusing on its different behavioral and neuronal assessments, aiming to provide a broad overview of common findings and limitations. Additionally, we explore the role of somato-sensory/somato-motor components and physiological markers underlying body image disturbances, which have not been extensively examined in previous studies. Literature screening was performed across three databases (PubMed, PsycINFO, Scopus). We classified behavioral, physiological, and neuroimaging studies according to their degree of involvement with body image dimensions and discussed the primary findings for each component. A total of 4015 studies were identified, of which 107 were eligible (84 behavioral and 23 neuroimaging studies) based on eligibility criteria, covering research from 1973 to 2024. Notably, of those which specified the following factors: 16 studies focused on the restrictive subtype (AN-r), two on the binge-eating/purging subtype (AN-b/p), and 19 examined both AN-r and AN-b/p; finally four on the atypical subtype; 11 on the weight-recovered state (WR) and three included also male participants. The mean age across all studies was 20.1 years (SD = 3.79). Our findings reveal that body image disturbances in AN are not limited to the overestimation of body size but also encompass cognitive, emotional, and somato-sensory aspects. A strong drive for thinness emerged as a prominent cognitive factor in maintaining the disturbance, along with altered autobiographical recall, executive function, and attentional biases toward body-related stimuli. Emotional regulation difficulties, altered interoceptive awareness, and distorted somato-sensory aspects—reflected in tactile and motor processes—were highlighted. In addition, increased autonomic reactivity to body image stimuli, indicating a sympathetic/parasympathetic imbalance, is supported by specific cortical-subcortical functional alterations. Our review emphasizes that body image distortion in AN should not be viewed merely as a perceptual deficit but rather as a complex cognitive-emotional and somato-sensory process.
Tea leaf exosome-like nanoparticles (TELNs) improve oleic acid-induced lipid metabolism by regulating miRNAs in HepG-2 cells
Authors: Lei Xuanhao , Li Haonan , Chen Sibei , Li Bing , Xia Huili , Li Jun , Guan Feng , Ge Jian 
Published: February 2025

Abstract
Tea is a widely consumed beverage globally, but the tea industry faces a significant waste management challenge. In this study, we developed tea leaf exosome-like nanoparticles (TELNs) with an average size of 274 ± 24.7 nm and a zeta potential of -20.6 ± 0.78 mV, using polyethylene glycol (PEG) 6000 precipitation followed by ultracentrifugation. Structural analysis confirmed that TELNs are composed of lipids, proteins, and RNAs. In vitro assays on HepG-2 cells revealed that TELNs are non-toxic at concentrations up to 300 µg/mL and can be efficiently internalized. TELNs exhibited significant antioxidant capacity and were able to significantly ameliorate H2O2-induced oxidative stress, increase the viability and reduce the accumulation of ROS in Hepg-2 cells. Notably, TELNs significantly alleviated OA-induced lipid metabolic disorders and hepatocellular injury. Further molecular analysis revealed that TELNs downregulated the expression of miR-21-5p, miR-17-3p, and miR-107, leading to the upregulation of their target genes PPARα, CYP7A1, and CPT-1A, which contributed to the improvement of lipid metabolism. This study is the first to demonstrate the lipid metabolism regulation potential of TELNs, providing new insights into their underlying mechanisms and helping to develop new therapeutic strategies for lipid metabolism-related diseases. Furthermore, it expands the scope of tea use and helps to reuse tea residues.

Graphical Abstract
Importance of portfolio optimization in SRI and conventional pension funds
Authors: Alda Mercedes 
Published: February 2025

Abstract
This study assesses the portfolio concentration of socially responsible investment (SRI) pension funds, which may be subject to a potentially limited asset universe and have a higher concentration and lower performance than conventional funds. Nonetheless, in contrast to previous studies on SRI funds, this study considers the information-advantage theory, positing that skilled managers should increase their concentration in assets in which they possess valuable information, departing from optimization models to achieve outperformance. This study first compares actual fund concentration with concentration obtained from several traditional and modern portfolio optimization techniques (minimum variance, global minimum variance, optimal portfolio, naïve diversification, risk parity, and reward-to-risk timing) to understand whether SRI pension funds concentrate portfolios and deviate from optimization model solutions. Unlike previous studies, the actual fund assets are considered in the optimization models to take into account the real investment profiles of SRI funds. The results indicate that SRI pension funds are less concentrated than conventional funds, and SRI and conventional pension funds largely diversify their portfolios, presenting lower concentration than portfolios formed with the optimization models. Furthermore, concentration strategies positively influence performance in SRI and conventional funds, revealing the use of information advantage. However, SRI and conventional fund managers present poor skills (picking, timing, and trading) to exploit information advantages due to overconfidence issues, which affect performance with concentration strategies. This situation may be modified if SRI funds follow modern optimization models and conventional funds follow traditional optimization models, improving managers’ performance and skills.
Who worries more Chinese or Russian university students? Measuring the correlations between socio-biographical variables and anxiety related to learning a foreign language
Authors: Borisova Anna Stepanovna , Aleksandrova Oksana Ivanovna , Moskvitcheva Svetlana Alekseevna , Soomro Muhammad Arif 
Published: February 2025

Abstract
The present study investigates the correlations between socio-biographical variables (such as age, gender, years of studying English language, and educational environment) and foreign language anxiety learning. The study also compares the manifestation of foreign language anxiety among Chinese and Russian university students. A mixed-method of both quantitative and qualitative design was employed to analyze gathered data from n = 240. The data were collected through a self-reported questionnaire—Foreign Language Anxiety Scale, adopted from Horwitz et al. (Modern Lang J 70:125–132, 1986), it was further supplemented with ethnographic observation. The findings indicate a negative relationship between foreign language anxiety and socio-biographical variables of university students. The findings reveal that Chinese students were more worried related to learning a foreign language in comparison to Russian university students. Whereas, Chinese and Russian male students experience more anxiety about foreign language learning in comparison to female Chinese and Russian students. The findings have implications in English as a foreign language, applied linguistics, teaching and learning environments, and anxiety studies.
Use of flaxseed cake as a source of nutrients in the food industry and possible health benefits- a review
Authors: Talwar Binanshu , Chopra Rajni , Taneja Neetu Kumra , Chand Monika , Homroy Snigdha , Dhiman Aishwarya , Singh Priyanka Kumari , Chaudhary Shreya 
Published: February 2025

Abstract
Oilseed cake is a potential by-product with a rich nutrient profile that can be used to develope value-added products. Flaxseed cake (FC), a by-product of flaxseed oil extraction, has gained considerable attention due to its diverse nutritional profile, bioactive constituents, and potential health benefits. It is an economical source of various bioactives, especially proteins, alpha-linolenic acid, lignan, flaxseed gum, and dietary fiber with potential health benefits. FC also contains residual fat which is a rich source of omega-3 fatty acids. Its high protein content and non-toxic nature make it a potential ingredient for use in human nutrition.
This review paper provides an overview of the nutritional profile of FC which includes macro and micronutrients as well as bioactive components such as lignans, phenolic compounds, and antioxidants along with their health-promoting properties. Due to the presence of antinutrients, processing is required for flaxseed cake which has been discussed. Furthermore, the health benefits linked to the consumption of FC are scrutinized.
Despite the positive attributes of FC, antinutrients namely cyanogenic glycosides, phytic acid, and tannins could restrict the absorption of nutrients. Several processing methods such as soaking, steaming, and fermentation have been proven to be beneficial in improving the nutritional quality of oilseed cake and the bioavailability of nutrients.

Graphical Abstract
The assessment of IL-6 in immune response to hepatitis B among hepatitis B vaccinated individuals in Specialist Hospital Sokoto State, North-Western Nigeria
Authors: Usman Abubakar Babangida , Muazu Safiyya , Ibrahim Adamu Muhammad , Funom Sabo Ezra , Smoklo Andrew Godwin , Umar Zulkifilu , Kalgo Mustapha Umar , Aliyu Kabiru Bello , Hamid Kabir Magaji 
Published: February 2025

Abstract

Background
Hepatitis B remains a major health burden with perinatal transmission being a key concern in endemic regions in countries like Nigeria. Vaccination is critical but the 5–10% non-response rate necessitates research into biomarkers like IL-6 that may predict immunogenicity. This study assessed hepatitis B vaccine response and the role of IL-6 in vaccinated individuals in Specialist Hospital, Sokoto State, Nigeria.

Methods
This cross-sectional study quantified IL-6 and hepatitis B surface antibody (HBsAb) levels in 50 vaccinated subjects in Sokoto, Nigeria, using ELISA and chemiluminescence immunoassays. Sociodemographic data including age, gender, and vaccine route were collected via questionnaires. Correlation analysis was performed between IL-6 and HBsAb levels to assess vaccine response prediction potential.

Results
Among the 50 participants, 84% demonstrated seroprotection based on adequate HBsAb titers, confirming the effectiveness of vaccination programs. However, 16% remained insufficiently immunized despite vaccination, highlighting interindividual variability warranting further investigation. IL-6 levels were predominantly normal (92%) with few (8%) exhibiting low levels, indicating appropriate inflammatory regulation overall. No sociodemographic factors emerged as significant predictors. A non-significant (p = 0.354) negative correlation (r = − 0.134) was found between IL-6 and HBsAb, suggesting IL-6 may not strongly predict hepatitis B vaccine response.

Conclusion
While protection rates align with WHO benchmarks, non-responders indicate a need to uncover immune and genetic mechanisms influencing vaccine outcomes through larger studies. The negative IL-6/HBsAb correlation warrants further analysis given IL-6’s regulatory role.
Setting up a fracture liaison service to assess the risk of osteoporotic fractures and treatment gap in Helwan University Hospitals
Authors: Abd El-Aziz Merhan Tarek , El Miedany Yasser , Fadel Mohamed Hussein , El-Hammady Dina Hatem , Fehr Amal 
Published: February 2025

Abstract

Background
In Egypt, despite the presence of numerous approved osteoporosis medications and many dual-energy X-ray absorptiometry scan facilities, only a small percentage of patients at high risk of sustaining a fragility fracture receive treatment. This significant “treatment gap” puts the highest-risk individuals at risk for developing fragility fractures. The aim of this study was to highlight patients at high risk of experiencing fragility fractures in the catchment area of Helwan University Hospitals by establishing a fracture liaison service and examining how they received their osteoporosis management and utilization of services.

Purpose
(1) To determine the treatment gap for men and postmenopausal women who have experienced a fragility fracture and (2) to look into the traits and risk factors for fractures of patients who show up with an index fragility fracture.

Results
This study included 28 individuals with fragility fractures, with 60.7% being female and 39.3% being male. The average age was 68.75 (SD ± 8.93) years. Twenty-five percent of males and 62% of postmenopausal women had an increased 10-year fracture risk [not including bone mineral density (BMD)]. A total of 83.3% of males and 62.6% of postmenopausal women did not get any osteoporosis medicines, despite being eligible for therapy. The Fracture Risk Assessment Tool score and BMD measurements at the hip and spine showed a significant correlation.
Fracture risk was significantly correlated with falls, sarcopenia, and functional disability.

Conclusion
In the Helwan University Hospitals catchment area, there exists a significant treatment gap for older adults in Egypt. The discrepancy appears to be linked to inadequate patient education and a poor diagnosis rate for osteoporosis.
Dekrety króla Zygmunta III w kwestiach swobód religijnych
Authors: Krawczuk Wojciech 
Published: February 2025

W Archiwum Narodowym w Krakowie przechowywana jest księga litewskiego Sądu Asesorskiego z lat 1589–1595, o sygnaturze 29/637/0/1.2/483. Jej odkrycia dokonał Henryk Lulewicz. Dokumenty zawarte w niej ukazują przebieg procesów w sądach asesorskich. Ponieważ księgi koronne tego sądu zostały spalone przez okupantów niemieckich po upadku Powstania Warszawskiego, jest to źródło bardzo ważne nie tylko dla sprawy Litwy ale i Korony. Przeprowadzono analizę kilku dekretów królewskich Zygmunta III wpisanych do tej księgi. Ukazują one bardzo wyważone podejście króla do kwestii swobód religijnych w początkach jego panowania.
Efficient Cloud Server Deployment Under Demand Uncertainty
Authors: Liu Rui Peng , Mellou Konstantina , Gong Evelyn Xiao-Yue , Li Beibin , Coffee Thomas , Pathuri Jeevan , Simchi-Levi David , Menache Ishai 
Published: February 2025

Problem definition: Cloud computing is a multibillion-dollar business that draws substantial capital investments from large companies such as Amazon, Microsoft, and Google. Large cloud providers need to accommodate the growing demand for computing resources while avoiding unnecessary overprovisioning of hardware and operational costs. The underlying decision processes are challenging, as they involve long-term hardware and infrastructure investments under future demand uncertainty. In this paper, we introduce the cloud server deployment problem. One important aspect of the problem is that the infrastructure preparation work has to be planned for before server deployments can take place. Furthermore, a combination of temporal constraints has to be considered together with a variety of physical constraints. Methodology/results: We formulate the underlying optimization problem as a two-stage stochastic program. After carefully examining the demand data and on-the-ground deployment operations, we distill two structural properties on deployment throughput constraints and provide tightness results on a convex relaxation of the second stage. Based on that, we develop efficient cutting-plane methods that exploit the special structure of the problem and can accommodate different risk measures. We test our algorithms with real production traces from Microsoft Azure and demonstrate sizeable cost reductions. We show empirically that the algorithms remain optimal even when the two properties are not fully satisfied. Managerial implications: Cloud supply chain operations were largely executed manually due to their complexity and dynamic nature. In this paper, we show that the key decision processes can be systematically optimized. In particular, we demonstrate that accounting for the stochastic nature of demands results in substantial cost reductions in cloud server deployments. Another benefit of our stochastic optimization approach is the ability to seamlessly integrate configurable risk preferences of cloud providers. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2023.0372 .