bluuebunny/crossref_metadata_2025_split huggingface dataset:
Embracing prospects for reducing the numbers of animals used in aquaculture research
Authors: Lazado Carlo C. , Ytteborg Elisabeth , Noble Chris 
Published: March 2024

AbstractThe principles of three Rs—REPLACEMENT, REDUCTION, and REFINEMENT—govern the protection and use of animals, including fish, for research purposes in the European Union and Norway. In this paper, we discuss some straightforward steps to simplify the delivery of these principles at the idea stage and adapt some of these examples for conducting fish trials related to health and welfare. Although some of the approaches are well established in other animal science arenas, we believe there can be a timely recap of their key facets. We discuss a number of simple strategies to emphasize how a reduction in fish numbers can be achieved from initial project conception to implementation, highlighting not only their advantages but also their limitations. We also highlight the role that funding agencies can play in the implementation of the 3R principles in aquaculture research. These simple points can be used in frameworks to initiate a broader and dynamic intersectoral dialogue among stakeholders of aquaculture research on how to promote ethics and embrace opportunities for this within the tenets of the 3Rs.
Kinetic modeling of valuable phenolic compounds extraction from strawberry and apple agro‐industrial by‐products
Authors: Villamil‐Galindo Esteban , Piagentini Andrea Marcela 
Published: March 2024

AbstractExtracting valuable compounds from waste plant tissues is a challenging process. To better understand this process, kinetic modeling was used to study the extraction of phenolic compounds from the waste tissues of industrial strawberry (RF) and apple (GS) processing using green solvents. The experimental design variables included type of solvent (W: water, EtOH: ethanol 80%), solid–liquid ratio (1:20, 1:30, 1:40), and temperature (20, 70°C). The model of Peleg (MI) and an empirical model (MII) were applied to determine the kinetic constants for total phenolic compounds (TPC) and antioxidant capacity (AC). The phenolic compound profile was determined at each experimental assay. The extraction variables affected (p < 0.001) TPC and AC of RF and GS extracts. Using EtOH increased TPC extraction yields from both tissues (up to 62% for RF). The highest AC was determined in EtOH extracts at 20°C (119 and 25 μmol TE/g for RF and GS, respectively). Temperature, extraction ratio, and solvent type significantly affected the kinetic constants of both models. The study identified nine phenolic compounds from both waste tissues, with Agrimoniin being the main compound in RF (0.49 g/kg) and quercetin pentoxide being the main compound in GS (0.23 g/kg). There was no significant increase in TPC and AC of the extracts after 90 min at 20°C and 60 min at 70°C. The kinetic modeling of phenolic compound extraction from strawberry and apple agro‐industrial by‐products using green solvents enabled the determination of the best extraction conditions, promoting the use of these waste tissues by obtaining bioactive compounds with antioxidant potential.Practical ApplicationsThis study provides valuable insight into the effective use of green solvents in extracting bioactive compounds, thereby promoting sustainable practices and reducing waste in the industrial processing of strawberries and apples. The kinetic modeling approach for recovering phenolic compounds from strawberry and apple agro‐industrial by‐products improves our comprehension of the influence of the extraction process variables on the phenolic compound profile and antioxidant capacity of these tissues. These findings highlight the potential of these waste tissues as an alternative source of bioactive compounds. By standardizing the extraction process and optimizing the utilization of agro‐industrial by‐products, the food industry can effectively reduce the environmental impact of these waste tissues and promote sustainable operations. Additionally, using green solvents aligns with the increasing demand for environmentally friendly practices, making it easier to turn strawberry and apple agro‐industrial processes into circular processes by eco‐friendly valorization of these biowastes.
Foreign Exchange Intervention: A Data Set of Official Data and Estimates
Authors: ADLER GUSTAVO , CHANG KYUN SUK , MANO RUI C. , SHAO YUTING 
Published: March 2024

AbstractA better understanding of foreign exchange intervention (FXI) is often hindered by the lack of data. This paper provides a new data set of FXI covering a large number of countries since 2000 at monthly and quarterly frequencies. It includes published official data for about 40 countries as well as carefully constructed estimates for 122 countries. Estimates account for a wide range of central bank operations, including both spot and derivative transactions. These estimates improve upon traditional proxies based on changes in reserves, by adjusting for valuation changes, income flows, and changes in other foreign‐currency balance sheet positions (both vis‐à‐vis residents and nonresidents)—the first estimates to do the latter to our knowledge—thus providing a more accurate measure of operations that change the central bank's foreign currency position. The data set also provides a classification of FXI operations into sterilized or not sterilized, a key dimension for economic analysis. Finally, the paper discusses the merits of the new estimates relative to traditional proxies, and presents stylized facts.
A framework for analysing water security of Tonle Sap Lake in the Mekong River basin: Flow, volume, inundation, productivity and livelihoods
Authors: Sithirith Mak 
Published: March 2024

AbstractWater is a scarce resource globally, and a water shortage worldwide would cause water security. Although there is plenty of literature studying water security, many discuss water security concerning water quality that is harmful to human health, some on the floods and droughts which destroy agriculture and livelihoods of people, and others on water war or water conflict in transboundary river basin; but little discusses water security concerning changing river flow, volume, inundation, productivity and livelihoods in the river–lake system (RLS). Thus, this paper studies the RLS to examine how changes in river flows and the volumes of the water in the RLS would affect the inundation and productivity of the RLS and, later, the livelihoods of river–lake people (RLP). In answering this question, the article undertakes the literature review and the case study of Tonle Sap Lake (TSL) in Cambodia to examine this form of water security. The paper concludes that the RLS has a close relationship between the flow, volume, inundation and productivity of fisheries and people's livelihoods. The hydropower dams, urban development and climate change cause the disconnectivity and the discontinuity of the river–lake flow, volume and inundation, undermining the productivity of fisheries and livelihoods of river–lake‐dependent communities. There is a strong need for cooperation and coordination between riparian states in the Mekong River Basin (MRB) to maintain the secured flow and volume in the Mekong River (MR) and TSL and protect the livelihoods of RLP.
Parental brain through time: The origin and development of the neural circuit of mammalian parenting
Authors: Kuroda Kumi O. , Fukumitsu Kansai , Kurachi Takuma , Ohmura Nami , Shiraishi Yuko , Yoshihara Chihiro 
Published: March 2024

AbstractThis review consolidates current knowledge on mammalian parental care, focusing on its neural mechanisms, evolutionary origins, and derivatives. Neurobiological studies have identified specific neurons in the medial preoptic area as crucial for parental care. Unexpectedly, these neurons are characterized by the expression of molecules signaling satiety, such as calcitonin receptor and BRS3, and overlap with neurons involved in the reproductive behaviors of males but not females. A synthesis of comparative ecology and paleontology suggests an evolutionary scenario for mammalian parental care, possibly stemming from male‐biased guarding of offspring in basal vertebrates. The terrestrial transition of tetrapods led to prolonged egg retention in females and the emergence of amniotes, skewing care toward females. The nocturnal adaptation of Mesozoic mammalian ancestors reinforced maternal care for lactation and thermal regulation via endothermy, potentially introducing metabolic gate control in parenting neurons. The established maternal care may have served as the precursor for paternal and cooperative care in mammals and also fostered the development of group living, which may have further contributed to the emergence of empathy and altruism. These evolution‐informed working hypotheses require empirical validation, yet they offer promising avenues to investigate the neural underpinnings of mammalian social behaviors.
Research trends between diabetes mellitus and bariatric surgery researches: Bibliometric analysis and visualization from 1998 to 2023
Authors: Yuan Kaisheng , Zhang Xu , Wu Bing , Zeng Ruiqi , Hu Ruixiang , Wang Cunchuan 
Published: March 2024

SummaryThis study conducted a bibliometric analysis using the Web of Science Core Collection (WOSCC) to explore the relationship between diabetes mellitus and bariatric surgery (BS) from January 1985 to August 2023. No publications were found between 1985 and 1998. However, from 1998 to 2023, a total of 9,496 English articles were identified, accumulating 291,289 citations (241,563 excluding self‐citations) and achieving an H‐Index of 197. Leading contributors to the field were the United States, China, and Italy. Noteworthy authors in this area of research included Philip R. Schauer, Wei‐Jei Lee, and Carel W. le Roux. The major journals that featured this research were ‘Obesity Surgery,’ ‘Diabetes Care,’ and ‘Surgery for Obesity and Related Diseases.’ The most highly cited article focused on lifestyle, diabetes, and cardiovascular risks 10 years after BS, emphasizing the significant attention given to the nutritional, cardiac, and general internal medicine impacts of diabetes and BS. The increase in research output during the review period indicates a growing interest in the relationship between diabetes and BS, providing a valuable reference for future studies in this evolving field.
Analysis of physiological response and differential protein expression of Paramichelia baillonii saplings under phosphorus deficiency
Authors: Ali Izhar , Wu Tong , Chen Kai , Ullah Saif , Xu Yuanyuan , Yang Mei 
Published: March 2024

AbstractParamichelia baillonii is a rare and fast‐growing tree species in subtropical China. The acidic red soil in southern China severely limits its growth as it lacks sufficient available phosphorus (P), resulting in declining soil fertility and nutrient availability. The effect of P deficiency on P. Baillonii growth, root attributes, and physiological response has not yet been reported. Understanding the adaptability of P. baillonii to low‐P conditions can improve afforestation and soil management in southern China. Therefore, we conducted a pot experiment on 2‐year‐old saplings and treated them with different P levels. Results showed that P deficiency (0–5 mg L−1) decreased growth attributes, root morphological traits, and nutrient uptake of P. baillonii saplings compared to control (CK). Similarly, reduction in chlorophyll a, b, total chlorophyll, net photosynthetic rate (Pn), transpiration rate (Tr), and Gs were seen in low P treatment saplings compared to CK. Whereas superoxide dismutase, peroxidase, malondialdehyde, acid phosphatase activity, and soluble protein content increased with increasing P‐deficiency up to 5 mg L−1, and soluble sugar showed oppsite trend. Moreover, the proteomics analysis identified 2721 proteins, 196 showing differential expression, with 90 up‐ and 106 down‐regulated. Importantly, the metabolic activities increased in the pentose phosphate pathway, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, and phenylpropanoid biosynthesis pathways to sustain regular plant growth under P deficiency. This study delves into the dynamic morpho‐physiological and proteomic changes in response to P deficiency. Overall, growth and nutrient uptake were reduced, countered by adaptive biochemical and proteomic shifts, including heightened antioxidant activities and modifications in metabolic pathways, highlighting the resilient strategies of P. baillonii saplings under P deficiency.
The sports industry and its effect on the quality of life and regional development
Authors: Zhai Zhihao , Du Manyu , Meng Hengran 
Published: March 2024

AbstractBackgroundSport plays a significant role in shaping social life, the health of the nation and the development of regions. Studying the impact of the sports industry on the quality of life and regional development can help to better understand how sports influence the social, economic and cultural aspects of people's lives.ObjectiveThis study aimed to investigate the impact of the sports industry on the quality of life and human development in developed and developing countries by analyzing the correlation and modeling of recognized world indices.MethodsThe research employed correlation analysis, regression analysis, and scenario modeling, utilizing publicly available international indices to determine the level of impact of sports on the country's development indicators. While investment in sports development may not be a straightforward decision for developing countries facing socio‐economic challenges, the study demonstrated the long‐term benefits of such investments through evidence‐based mathematical regression models and scenario modeling.ResultsThe study findings revealed that the advancement of the sports industry leads to improvements in the quality of life and human development in most of the studied regions.ConclusionMoreover, the scenario modeling suggested that the evolution of this field would further enhance the development of the quality of life.
Successful treatment of severe passenger lymphocyte syndrome with efgartigimod synergy
Authors: Chase R. Christopher , Koop Andree H. , Shaikh Marwan , Imperial Robin J. , Harnois Denise M. , Loo Nicole M. , O'Brien Jennifer J. 
Published: March 2024

AbstractIntroductionThis case describes passenger lymphocyte syndrome (PLS) generating human platelet antigen 1a (HPA‐1a) alloantibodies against the recipient's platelets after liver transplant. Given the rarity of PLS, especially in liver transplant with HPA‐1a alloantibodies, disease course and management options are poorly described.MethodsThe patient had cirrhosis secondary to nonalcoholic steatohepatitis complicated by hepatocellular carcinoma, encephalopathy, and severe ascites. The model for end‐stage liver disease (MELD) score was 15 at presentation. The patient developed hepatic artery thrombosis after an orthotopic liver transplant and was relisted for transplant with a MELD score of 40. The patient received a hepatitis C virus antibody positive, hepatitis C virus nucleic amplification test positive donor liver on postoperative day (POD) 7 after first transplant. On POD 7 after the second transplant, the patient developed profound thrombocytopenia refractory to platelet infusion. They were found to have serum antibody to HPA‐1a based upon serum platelet alloantibody testing. The donor was later found to be negative for HPA‐1a by genetic testing. However, the patient's native platelets were HPA‐1a positive. The patient was diagnosed with PLS.ResultsThe patient's treatment course included 57 units of platelets transfused, emergency splenectomy, rituximab, plasma exchange, intravenous immunoglobulin (IVIG), eltrombopag, romiplostim, and efgartigimod.DiscussionThe synergistic effect of efgartigimod with eltrombopag and romiplostim most likely resolved the patient's thrombocytopenia. This case represents a novel use of efgartigimod in the treatment of passenger lymphocyte syndrome following liver transplant.
Establishment of clinical criteria for the diagnosis of adverse food reactions in dogs with atopic dermatitis
Authors: Sofou Evi I. , Aleksandrova Svetlina , Chatzis Manolis , Samuel (Badulescu) Elisa , Saridomichelakis Manolis N. 
Published: March 2024

AbstractBackgroundDiagnosis of canine adverse food reactions (AFRs) is based on vague criteria, such as ‘>50% improvement’ during elimination diet trial (EDT) followed by ‘deterioration’ during provocation test (PT).ObjectiveThe objective of the study was to use predefined criteria to evaluate response during EDT [i.e., Owner Global Assessment of Treatment Efficacy (OGATE) = good‐to‐excellent] and relapse during PT [i.e., Owner Global Assessment of Challenge Deterioration (OGACD) = moderate‐to‐severe and/or >100% increase of lesional (Canine Atopic Dermatitis Extent and Severity Index, 4th iteration, CADESI‐04) and/or of pruritus (pruritus Visual Analog Scale, PVAS) scores].AnimalsTwenty‐nine dogs with atopic dermatitis.Materials and MethodsAn extensively hydrolysed diet was fed to all dogs followed, in seven of 11 nonresponders, by a second home‐made novel‐protein EDT. Dogs responding to either EDT were challenged with their previous diet.ResultsThirteen (44.8%) dogs were diagnosed with AFRs: at the end of EDT, their OGATE was good (9 of 13; 69.2%) or excellent (four of 13; 30.8%), and both CADESI‐04 (46.7%) and PVAS (71.1%) had decreased significantly; at the end of PT, OGACD was moderate or severe in 12 of 13 (92.3%) dogs, and both CADESI‐04 (127.9%) and PVAS (181.8%) had increased significantly. Of the 16 dogs without AFRs, 6 (37.5%) responded to the commercial (n = 5) or home‐made (n = 1) diet [OGATE = good (three of six) or excellent (three of six)], with significant concurrent reduction of CADESI‐04 and nonsignificant reduction of PVAS, yet they did not relapse during PT.Conclusions and Clinical RelevanceThe proposed (predefined) criteria for the evaluation of response during EDT and deterioration during PT seem reliable and are easily applicable in clinical practice and research.
16S rRNA amplicon sequencing of seawater microbiota from Rayong Province, Thailand, affected by oil spills
Authors: Pinpimai Komkiew , Punnarak Porntep , Thitiphuree Tongchai , Songploy Se , Panichpol Arnupap , Chankong Anchalee , Chavanich Suchana , Viyakarn Voranop 
Published: March 2024

ABSTRACT

We reported on microbial communities isolated from 18 seawater samples affected by oil spills in Rayong province, Thailand, using the V3-V4 region of 16S rRNA gene sequencing.
Carbapenem-resistant
Acinetobacter baumannii
carrier detection: a simple and efficient protocol
Authors: Lellouche Jonathan , Keren-Paz Alona , Rov Reut , Efrati Epchtien Reut , Frenk Sammy , Hameir Amichay , Temkin Elizabeth , Schwartz David , Carmeli Yehuda 
Published: March 2024

ABSTRACT

Timely detection of carbapenem-resistant
Acinetobacter baumannii
(CRAB) carriers is essential to direct infection control measures. In this work, we aimed to develop a practical protocol to detect CRAB from screening samples. To choose a selective medium that detects CRAB with high sensitivity and specificity, 111
A
.
baumannii
clinical isolates were inoculated on three types of agar: mSuperCARBA (SC), CHROMagar Acinetobacter (CaA), and modified CHROMagar Acinetobacter (mCaA) containing 4.5 mg/mL meropenem. SC was non-selective, CaA was the most sensitive (100%), but only moderately specific (72%), and mCaA was highly specific (97%) and sensitive (98%). Confirmation of the carbapenem-resistant phenotype using PCR-based detection of
bla
OXA-23
,
bla
OXA-24
, and
bla
OXA-58
genes was specific but not sensitive, detecting only 58% of CRAB isolates. Identification of
A. baumannii
using either
gyrB
or
bla
OXA-51
PCR was excellent. Next, we used the same methodology in routine screening for CRAB carriage. mCaA had the best yield, with high sensitivity but moderate specificity to differentiate between CRAB and other carbapenem-resistant organisms. Skin sampling using sponges and 6 hour enrichment was highly sensitive (98%), while other body sites had poor sensitivity (27%– 41%). Shorter incubation had slightly lower yield, and longer incubation did not improve the detection. Performing PCR for
bla
OXA-51
and
gyrB
on colonies growing on modified mCaA differentiated between CRAB and other species with high accuracy (98% and 99%, respectively). Based on our results, we present a procedure for easy and reliable detection of CRAB carriage using skin sampling, short enrichment, selection on mCaA, and PCR-based identification.

IMPORTANCE

Carbapenem-resistant
Acinetobacter baumannii
(CRAB) is a substantial cause of nosocomial infections, classified among the most significant multidrug-resistant pathogens by the World Health Organization and by the US Centers for Disease Control. Limiting the spread of CRAB is an important goal of infection control, but laboratory methods for identification of CRAB carriers are not standardized. In this work, we compared different selective agar plates, tested the efficiency of
A. baumannii
identification by PCR for species-specific genes, and used PCR-based detection of common resistance genes to confirm the carbapenem-resistant phenotype. During a prospective study, we also determined the optimal sample enrichment time. Based on our results, we propose a simple and efficient protocol for the detection of CRAB carriage using skin sampling, short enrichment, selection on appropriate agar plates, and PCR-based identification, resulting in a turn-around time of 24 hours.
Zooplankton of the Eastern Kara Sea: Response to a Short Ice-Free Period
Authors: Drits A. V. , Pasternak A. F. , Arashkevich E. G. , Amelina A. B. , Shchuka T. A. , Flint M. V. 
Published: March 2024

Abstract—
A characteristic feature of the eastern Kara Sea, which is separated from its central part by the shallow Central Kara Rise, is a later ice breakup, a shorter ice-free period, and lower water temperature. Zooplankton in this part of the sea is virtually unstudied. The first data on the composition, spatial distribution of zooplankton, age structure and feeding of the dominant species, and their grazing impact on phytoplankton were obtained in the cruise 125 of the R/V Professor Shtokman (September 2013) and cruise 63 of the R/V Akademik Mstislav Keldysh (September 2015) to the eastern Kara Sea. 2015 was a typical year in terms of the ice regime for this region, while in 2013, seasonal ice coverage lasted longer than usual. Zooplankton biomass (17–112 mg dry weight m–3), to which copepods Calanus glacialis contributed the main portion, were close to the values in the central Kara Sea in September. The seasonal development of the C. glacialis population began much later than in the central Kara Sea, but development of Metridia longa was not influenced by the specifics of the ice regime and hydrophysical conditions in the eastern Kara Sea. The interannual differences in the timing of ice retreat had almost no effect on the zooplankton abundance, seasonal development of the dominant copepod populations, feeding activity, and grazing impact on phytoplankton.
Sedimentation Processes and the Holocene Evolution of the Indigirka Paleovalley (Coastal Shelf Area of the East Siberian Sea)
Authors: Ryabchuk D. V. , Taldenkova E. E. , Sergeev A. Yu. , Zhamoida V. A. , Budanov L. M. , Prishchepenko D. V. , Nosevich E. S. , Terekhina Ya. E. , Bashirova L. D. , Ponomarenko E. P. , Neevin I. A. , Chekulaev A. V. , Grigoriev A. G. 
Published: March 2024

Abstract
Problems of the paleogeographical evolution and changes in sedimentation environment within the Indigirka River paleovalley (the East Siberian Sea) are discussed. Based on the results of geophysical and geological research and a high-resolution study of sediment cores (grain size analysis, geochemistry, macro- and micropaleontology, pollen analysis, radiocarbon dating) the development of the Holocene marine transgression has been reconstructed, sedimentation rates have been calculated, and the basic principles of changes in sedimentation processes have been established.