bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Pralsetinib in Human Plasma and Cerebrospinal Fluid for Therapeutic
Drug Monitoring by Ultra-performance Liquid Chromatography-Tandem Mass
Spectrometry (UPLC-MS/MS)
Authors: Zhao Zichen , Pu Qianlun , Sun Tonglin , Huang Qian , Tong Liping , Fan Ting , Kang Jingyue , Chen Yuhong , Zhang Yan 
Published: April 2024

Background:
Ultra-performance Liquid Chromatography-tandem Mass Spectrometry (UPLC-MS/MS)
is widely used for concentration detection of many Tyrosine Kinase Inhibitors (TKIs), including afatinib, crizotinib,
and osimertinib. In order to analyze whether pralsetinib takes effect in Rearranged during Transfection
(RET)-positive patients with central nervous system metastasis, we aimed to develop a method for the detection
of pralsetinib concentrations in human plasma and Cerebrospinal Fluid (CSF) by UPLC-MS/MS.

Methods:
The method was developed using the external standard method, and method validation included precision,
accuracy, stability, extraction recovery, and matrix effect. Working solutions were all obtained based on
stock solutions of pralsetinib of 1mg/mL. The plasma/CSF samples were precipitated by acetonitrile for protein
precipitation and then separated on an ACQUITY UPLC HSS T3 column (2.1×100 mm, 1.8 μm) with a gradient
elution using 0.1% formic acid (solution A) and acetonitrile (solution B) as mobile phases at a flow rate of 0.4
mL/min. The tandem mass spectrometry was performed by a triple quadrupole linear ion trap mass spectrometry
system (QTRAPTM 6500+) with an electrospray ion (ESI) source and Analyst 1.7.2 data acquisition system.
Data were collected in Multiple Reaction Monitoring (MRM) and positive ionization mode.

Results:
A good linear relationship of pralsetinib in both plasma and CSF was successfully established, and the
calibration ranges were found to be 1.0-64.0 μg/mL and 50.0ng/mL-12.8 μg/mL for pralsetinib in the plasma and
CSF, respectively. Validation was performed, including calibration assessment, selectivity, precision, accuracy,
matrix effect, extraction recovery, and stability, and all results have been found to be acceptable. The method has
been successfully applied to pralsetinib concentration detection in a clinical sample, and the concentrations have
been found to be 475 ng/mL and 61.55 μg/mL in the CSF and plasma, respectively.

Conclusion:
We have developed a quick and effective method for concentration detection in both plasma and
CSF, and it can be applied for drug monitoring in clinical practice. The method can also provide a reference for
further optimization.
Kawasaki Disease and Respiratory Viruses: Ecological Spatiotemporal Analysis
Authors: Sawires Rana , Clothier Hazel J , Burgner David , Fahey Michael Collingwood , Buttery Jim 
Published: April 2024

Abstract

Background
Kawasaki disease is an uncommon vasculitis affecting young children. Its etiology is not completely understood, although infections have been frequently postulated as the triggers. Respiratory viruses, specifically, have often been implicated as causative agents for Kawasaki disease presentations.

Objective
We aimed to conduct an ecological spatiotemporal analysis to determine whether Kawasaki disease incidence was related to community respiratory virus circulation in a shared region and population, and to describe viral associations before and during the COVID-19 pandemic.

Methods
We obtained independent statewide data sets of hospital admissions of Kawasaki disease and respiratory multiplex polymerase chain reaction tests performed at two large hospital networks in Victoria, Australia, from July 2011 to November 2021. We studied spatiotemporal relationships by negative binomial regression analysis of the monthly incidence of Kawasaki disease and the rate of positive respiratory polymerase chain reaction tests in different regions of Victoria. Peak viral seasons (95th percentile incidence) were compared to median viral circulation (50th percentile incidence) to calculate peak season increased rate ratios.

Results
While no seasonal trend in Kawasaki disease incidence was identified throughout the study period, we found a 1.52 (99% CI 1.27‐1.82) and a 1.43 (99% CI 1.17‐1.73) increased rate ratio of Kawasaki disease presentations in association with human metapneumovirus and respiratory syncytial virus circulation, respectively, before the COVID-19 pandemic. No respiratory viral associations with Kawasaki disease were observed during the COVID-19 pandemic.

Conclusions
Our large ecological analysis demonstrates novel spatiotemporal relationships between human metapneumovirus and respiratory syncytial virus circulation with Kawasaki disease. The disappearance of these associations in the COVID-19 pandemic may reflect the reduced circulation of non–SARS-CoV-2 viruses during this period, supporting the prepandemic associations identified in this study. The roles of human metapneumovirus and respiratory syncytial virus in Kawasaki disease etiology warrant further investigation.
Construcciones sociales de la salud sexual en mujeres adolescentes de una comunidad rural
Authors: Torres-Camarillo Diego Alonso , Lara-Díaz Cristina Judith , Gallegos-Ortiz Ana Emmily , Espitia-López Jazmin Zurisadai , Altamira-Camacho Ramiro 
Published: April 2024

Objetivo: Indagar cuáles son las construcciones sociales en la salud sexual de las mujeres adolescentes de una comunidad rural. Material y Métodos: Estudio cualitativo fenomenológico hermenéutico, donde participaron mujeres adolescentes de 14 a 19 años, pertenecientes a la comunidad, El Molino, Ojuelos Jalisco. A través de un muestreo por cadena de referencia, se realizaron entrevistas fenomenológicas, transcritas de manera artesanal y analizadas mediante el “círculo hermenéutico Heiderggariano”. Se obtuvo la autorización mediante el consentimiento informado, así como el asentimiento por parte de los tutores. Cumpliendo un rigor metodológico a través de la trasparencia, transferibilidad, credibilidad y autenticidad. Resultados: se realizaron 10 entrevistas afines a 10 adolecentes, se determinaron 6 unidades de significado, de los cuales tres son ónticas; una realidad palpable a el desconocimiento de métodos anticonceptivos, ideologías paternales adiestradas y falocentrismo como base central en la dinámica social. Y tres son ontológicas; Sesgo educativo, Discurso del lenguaje culturizado, finalmente tabúes y estigmas familiares. Conclusión: Las mujeres adolescentes de una comunidad rural vivencian una doble moral social. Demostrando así, un ineficiente conocimiento sobre salud sexual hasta ser un tema intrascendente, los padres son quienes influyen y toman decisiones sobre la educación, autonomía y forma de pensar.
Palabras clave: Feminismo; Sexualidad; Enfermería; Genero.
An in-vitro study on effects of laser activation on dye penetration in human root dentin
Authors: Anton y Otero Clara Isabel , Marger Laurine , Di Bella Enrico , Feilzer Albert , Krejci Ivo , Abdelaziz Marwa 
Published: April 2024

Objective: To evaluate the penetration of a dye in root dentin after activation with different laser wavelengths.
Materials: Palatal roots of 38 human molars were enlarged and disinfected. Irrigation activation was performed with an Er:YAG laser: @50 mJ, 15 Hz (Er:YAG); a 9.3 µm CO2 laser: @40% power (CO2); diode lasers 455 nm/970 nm: @0.8 W, 15 Hz (D455, D970) and 808/980 nm: @1 W (D808, D980) and compared to positive control: etching with 35% H3PO4 (POS); negative control: water (NEG) and conventional needle irrigation: NaOCl and ethylenediaminetetraacetic acid (EDTA) (CONV). Methylene blue solution was introduced in the canal and laser-activated or left untouched for 100 s before the roots were dried and cut into horizontal slices. Dye penetration was automatically calculated by color recognition of two samples per root third (n = 8 per group in each coronal, middle and apical root thirds). The presence and absence of a smear layer was checked in two additional samples of the negative and positive control under scanning electron microscopy (SEM).
Results: Full-depth infiltration was not achieved in any group. Dye penetration in CONV was significantly less than in Er:YAG, CO2, POS, D455, D970, D808 and similar to NEG and D980 when results of different root thirds were pooled.
Conclusion: Laser activation using certain parameters enhanced dye penetration compared to conventional needle irrigation with NaOCl and EDTA (CONV).
Prognostic value of the intraoperative D-dimer level in arterial blood in open reconstructive operations on the aortoiliac segment
Authors: Zaitseva Yu. А. , Bedrov А. Ia. , Moiseev А. А. , Kadinskaia М. I. , Kovalchuk Iu. P. , Rybakov G. V. , Veremiichuk V. Iu. , Khubulava G. G. 
Published: April 2024

The objective was to assess the prognostic value of D-dimer level in peripheral venous blood and arterial blood to the risk of developing complications in the short-term period after open reconstructive surgery on the aortoiliac segment.Methods and materials. The study included 89 patients who were determined the D-dimer level in peripheral venous blood, as well as arterial blood intraoperatively.Results. Statistically significant differences in the D-dimer level were established depending on the presence or absence of early postoperative complications.Conclusions. The intraoperative D-dimer level in arterial blood has a predictive ability in relation to the development of early postoperative complications.
Psychological aspect of pain syndrome in endometriosis
Authors: Isaeva Е. R. , Vandeeva Е. N. , Pakhomova F. V. , Vorotnikova А. V. , Knizhnikova А. А. 
Published: April 2024

Relevance. Endometriosis is a multifactorial gynecological disease characterized by a benign proliferation of endometrial cells outside the uterine cavity. At the present stage of the study of endometriosis, the etiology and pathogenesis of its development have not been studied enough. More and more attention is paid to the emotional and personal characteristics of patients, and the disease itself is gradually moving into the category of psychosomatic disorders.The objective was to identify the features of the perception of pain syndrome, as well as emotional, personal characteristics and the level of adaptation of women with endometriosis.Methods and materials. The study involved 76 respondents: 46 women with endometriosis and 30 women without gynecological pathology (conditionally). We used psychodiagnostic questionnaire methods aimed at studying the perception of pain syndrome, emotional sphere, level of adaptation, personal characteristics and gender identity.Results. The personal profile of women with endometriosis is dominated by such traits as: anxiety, depression, difficulties in controlling life events, a decrease in the level of socio-psychological adaptation and satisfaction with the quality of life, a tendency to escape from reality in comparison with the personal profile of conditionally healthy women. It has been established that a higher assessment of the severity of pain syndrome in endometriosis is interconnected with such types of interpersonal interaction as «dependent-obedient» and «cooperating-conventional».Conclusions. The treatment and rehabilitation of patients with endometriosis requires taking into account their psychological characteristics, as well as a multidisciplinary approach involving specialists of various profiles, including psychologists/ psychotherapists.
Fatal convexity and interhemispheric acute subdural hematoma from a falx meningioma: A case report
Authors: Tanaka Tatsuya , Kyaw May Pyae , Anai Satoshi , Takase Yukinori , Takase Yukari , Abe Tatsuya , Matsuno Akira 
Published: April 2024

Background:
Hemorrhagic meningiomas are rare. We report a rare case of nontraumatic convexity and interhemispheric acute subdural hematoma (ASDH) caused by a falx meningioma.

Case Description:
An 84-year-old woman with a history of atrial fibrillation and hypertension who was taking warfarin presented to our emergency department with a sudden disorder of consciousness. The patient had no traumatic events associated with her symptoms. Computed tomography (CT) revealed right convexity and interhemispheric ASDH, mass lesions in the left frontal lobes, and brain herniation. Contrast-enhanced CT revealed vascular structures within the mass lesion. CT angiography (CTA) revealed no aneurysm or arteriovenous malformation, and the venous phase revealed occlusion in the anterior portion of the superior sagittal sinus. The patient had her right convexity and interhemispheric ASDH removed endoscopically. A mass lesion located on the falx, which was easily bleeding, soft, and suctionable, was immediately detected. Histopathological examination revealed fibrous meningioma, a benign meningioma of the World Health Organization grade 1. Despite undergoing aggressive treatment, the patient’s general condition deteriorated.

Conclusion:
Hemorrhagic meningiomas can easily be missed with plain CT, and the enhancement effect of CTA and tumor shadow on digital subtraction angiography may not be observed during the acute phase. Surgery for nontraumatic ASDH should be performed considering the possibility that a meningioma causes it.
Experiencia en evaluación: territorio con huellas
Authors: Maurel María del Carmen , Monti Cristina Mónica 
Published: April 2024

El artículo surge del proyecto de investigación “La evaluación: una herramienta para la mejora de la práctica pedagógica”. El estudio se realizó en las cátedras Física I y Algoritmos y estructura de datos de la carrera de Ingeniería en Sistemas de Información de la Universidad Tecnológica Nacional. El trabajo consiste en una indagación empírica de carácter cualitativo. Para el análisis de los datos, se utilizaron diferentes herramientas metodológicas: Análisis documental, relatos de las prácticas en forma de entrevistas y encuestas, observaciones de aulas virtuales y talleres de validación de resultados. Entre las conclusiones más significativas, surge que hay profesores que manejan cierto dominio conceptual sobre la evaluación formativa, sin embargo, se percibe que ese conocimiento no garantiza en muchos casos la transformación de sus prácticas evaluadoras. Hay propuestas metodológicas interesantes al respecto, pero su ejecución en el ámbito académico se ve muy acotada favoreciendo prácticas evaluativas cotidianas, rutinarias y estrechas.
Saúde, Sociedade e Meio Ambiente: ensaios preliminares
Authors: Santos Alethele de Oliveira , Barros Fernando Passos Cupertino de , Alves Juliane A. 
Published: April 2024

O momento em que se intensifica o debate mundial em torno da Agenda 2030 e os Objetivos do Desenvolvimento Sustentável (ODS) coincide com a ocorrência de pandemias que vêm causando graves danos em todo o mundo e desafiam a humanidade sobre o modo de como enfrentá-las, além de obrigar-nos a refletir e a agir rapidamente sobre suas causas e consequências. Elas chegam em um cenário mundial em que as pessoas conhecem e vivenciam, cada vez mais de perto, os efeitos nefastos de suas ações sobre o ambiente em que vivem. Ações e modos de vida que resultam em desigualdades estruturais, exploração econômica, migração crescente, mudanças climáticas e perda de biodiversidade, e até mesmo crimes ambientais.

Nesse contexto, movimentos fortes e determinados devem revestir-se de caráter imediato na busca pela transformação das sociedades, das economias e da relação humana com o meio ambiente. Por outro lado, as intervenções transformadoras, sejam elas políticas ou programáticas, necessitam ser avaliadas por meio de abordagens também abrangentes e, portanto, comprometidas com a análise dos seus efeitos sobre a saúde, a economia, a cultura e o meio ambiente, considerando igualmente os ciclos demográficos em diferentes regiões do mundo.

Atentos a esse cenário de transformações necessárias às intervenções, entre as quais devem estar incluídas investigações e avaliações da sua implementação e efetividade, a Linha Editorial Internacional de Apoio aos Sistemas de Saúde – LEIASS apresenta a seus leitores uma contribuição para o amadurecimento dessas diretrizes. Esperamos, por meio desta publicação, voltada à divulgação de ensaios teóricos e proposições metodológicas, que ela contribua para a conciliação e a integração de dimensões aparentemente desconexas dessas ações e que, diante da complexidade dos desafios nacionais e globais, ilumine esse debate.

Esta edição é, portanto, composta por textos de colaboradores, docentes e pesquisadores, que apresentam ensaios, reflexões e estudos relativos à saúde global e às inter-relações estabelecidas entre o modo de viver das sociedades e os sistemas naturais. Ainda, abordam intervenções cujas perguntas avaliativas demonstram a amplitude e a abrangência que consideram a complexidade de suas dimensões.
Características morfogênicas de cultivares do capim buffel submetidos à adubação nitrogenada
Authors: Porto Edson Marcos Viana , Vitor Claudio Manoel Teixeira , Alves Dorismar David , Lima Marcus Vinícius Gonçalves , Silva Marcos Ferreira da 
Published: April 2024

Informações sobre a morfogênese das gramíneas forrageiras utilizadas na região semiárida, assim como sua resposta à adubação nitrogenada, podem gerar subsídios para o manejo sustentável da produção a pasto nestas regiões. O objetivo deste estudo foi avaliar as características morfogênicas de cultivares de Cenchrus ciliaris submetidos à adubação nitrogenada. O delineamento experimental utilizado foi em blocos casualizados em arranjo fatorial 2 x 3 x 4 (duas épocas - verão e outono; três cultivares de C. ciliaris - Grass, PI 295658 e Áridus e quatro doses de nitrogênio - 0, 75, 150 e 225 kg ha-1), com três repetições. As características morfogênicas e estruturais foram mensuradas duas vezes por semana utilizando-se régua milimetrada, durante todo período experimental, em três perfilhos marcados aleatoriamente por unidade experimental, totalizando 144 perfilhos. A TAlF e a TAlPC forão influenciados, positivamente, pela adubação nitrogenada dos cultivares de Cenchrus ciliaris na época do verão. O filocrono, DVF, e a TApF não são influenciados pela adubação nitrogenada. Os cultivares avaliados não apresentaram diferença quanto a DVF e a TAlPC.
Algodoeiro herbáceo submetido a déficit hídrico: Produção
Authors: Barbosa de Almeida Érica Samara Araújo , Pereira José Rodrigues , Vieira de Azevedo Carlos Alberto , Araújo Whéllyson Pereira , Zonta João Henrique , Cordão Mailson Araújo 
Published: April 2024

Objetivou estudar o efeito de períodos de déficits hídricos, em diferentes fases fenológicas, sob os componentes da produção de cultivares de algodoeiro herbáceo, em experimento conduzido em casa de vegetação, com tratamentos distribuídos no delineamento experimental inteiramente casualizado em esquema fatorial 4 x 2, sendo os fatores quatro períodos de déficits de irrigação aplicados em diferentes fases fenológicas (D1= testemunha irrigada, D2 = déficit na fase de aparecimento do primeiro botão floral, D3 = déficit na fase de aparecimento da primeira flor e D4 = déficit na fase de aparecimento da primeira maçã) e 2 cultivares de algodoeiro herbáceo (BRS 286 e BRS 336) com 4 repetições. Os dados foram submetidos à análise de variância através do teste F, sendo as médias dos tratamentos dos fatores comparadas pelo teste de tukey a 5% de probabilidade. Os déficits hídricos aplicados nas diferentes fases fenológicas das cultivares BRS 286 e BRS 336 do algodoeiro herbáceo afetaram o número de capulho por planta e a produtividade; O tratamento sem déficit promoveu maior número de capulhos e produtividade para estas cultivares de algodoeiro herbáceo; O algodoeiro herbáceo foi mais tolerante ao déficit na fase de maçã; A cultivar BRS 336 se destacou com maiores valores de peso de capulho e percentagem de fibra.
AVALIAÇÃO DA ATIVIDADE ANTIMICROBIANA “IN VITRO” DA Lippia sidoides CHAM SOBRE Staphylococcus aureus DE ORIGEM BOVINA
Authors: Silva Viviane Araújo da , Freitas Andréia Fernanda Ramos de , Pereira Maria do Socorro Vieira , Pereira Andréia Vieira 
Published: April 2024

Staphylococus aureus apresenta-se como um microorganismo patogênico clássico sendo comumente reconhecido como agente etiológico de infecções hospitalares e comunitárias. Através do conhecimento das propriedades biológicas do Alecrim-pimenta (Lippia sidoides. Cham), esta pesquisa teve como objetivo avaliar a atividade antimicrobiana “in vitro” do óleo essencial desta planta em inibir o crescimento de cepas de S. aureus isoladas de secreções do úbere, fossas nasais e do leite. Os resultados mostraram destacável atividade anti-estafilococicas aureus do extrato da Lippia sidoides Cham notadas por halos de inibição do crescimento bacteriano (11 a 26 mm). A concentração inibitória mínima (CIM) encontrada foi de 1:8 para todas as amostras estudadas. A CIM apresentou eficácia na inibição da viabilidade de S. aureus em caldo durante vinte e quatro horas de interação, caracterizando efeito bacteriostático. Esses resultados demonstram a atividade anti-estafilococicas do extrato de Lippia sidoides, bem como, suportam a possibilidade de seu uso como agente antimicrobiano.
Novel Genetic and Biochemical Insights into the Spectrum of NEFL-Associated Phenotypes
Authors: Della Marina Adela , Hentschel Andreas , Czech Artur , Schara-Schmidt Ulrike , Preusse Corinna , Laner Andreas , Abicht Angela , Ruck Tobias , Weis Joachim , Choueiri Catherine , Lochmüller Hanns , Kölbel Heike , Roos Andreas 
Published: April 2024

Background: NEFL encodes for the neurofilament light chain protein. Pathogenic variants in NEFL cause demyelinating, axonal and intermediate forms of Charcot-Marie-Tooth disease (CMT) which present with a varying degree of severity and somatic mutations have not been described yet. Currently, 34 different CMT-causing pathogenic variants in NEFL in 174 patients have been reported. Muscular involvement was also described in CMT2E patients mostly as a secondary effect. Also, there are a few descriptions of a primary muscle vulnerability upon pathogenic NEFL variants. Objectives: To expand the current knowledge on the genetic landscape, clinical presentation and muscle involvement in NEFL-related neurological diseases by retrospective case study and literature review. Methods: We applied in-depth phenotyping of new and already reported cases, molecular genetic testing, light-, electron- and Coherent Anti-Stokes Raman Scattering-microscopic studies and proteomic profiling in addition to in silico modelling of NEFL-variants. Results: We report on a boy with a muscular phenotype (weakness, myalgia and cramps, Z-band alterations and mini-cores in some myofibers) associated with the heterozygous p.(Phe104Val) NEFL-variant, which was previously described in a neuropathy case. Skeletal muscle proteomics findings indicated affection of cytoskeletal proteins. Moreover, we report on two further neuropathic patients (16 years old girl and her father) both carrying the heterozygous p.(Pro8Ser) variant, which has been identified as 15% somatic mosaic in the father. While the daughter presented with altered neurophysiology,neurogenic clump feet and gait disturbances, the father showed clinically only feet deformities. As missense variants affecting proline at amino acid position 8 are leading to neuropathic manifestations of different severities, in silico modelling of these different amino acid substitutions indicated variable pathogenic impact correlating with disease onset. Conclusions: Our findings provide new morphological and biochemical insights into the vulnerability of denervated muscle (upon NEFL-associated neuropathy) as well as novel genetic findings expanding the current knowledge on NEFL-related neuromuscular phenotypes and their clinical manifestations. Along this line, our data show that even subtle expression of somatic NEFL variants can lead to neuromuscular symptoms.