bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astragaloside IV restrains pulmonary fibrosis progression via the circ_0008898/miR‐211‐5p/HMGB1 axis
Authors: Zhu Min , Zhao Limin , Zhang Xueying , Zhao Ruijuan 
Published: March 2024

AbstractPulmonary Fibrosis (PF) is a fatal lung disease with complicated pathogenesis. Astragaloside IV (ASV) has been discovered to alleviate PF progression, and the potential molecular mechanism of ASV in the development of PF need to be further clarified. Bleomycin (BLM) was used to construct PF in vivo model. Expression levels of circ_0008898, miR‐211‐5p, high mobility group protein B1 (HMGB1), alpha smooth muscle Actin (α‐SMA) and Collagen I were examined by Quantitative real time polymerase chain reaction (qRT‐PCR) and western blot. Cell survival was analyzed using Cell Counting Kit‐8 (CCK‐8) and EdU (5‐ethynyl‐2′‐deoxyuridine) assay. The invasion abilities were investigated by transwell assay. The levels of inflammatory factors were tested via using Enzyme‐linked immunosorbent assay (ELISA). The relationship between circ_0008898 or HMGB1 and miR‐211‐5p was identified by dual‐luciferase reporter assay. The results showed that ASV attenuated BLM‐induced pulmonary fibrosis in vivo. In vitro study, ASV alleviated TGF‐β1‐induced fibrogenesis in HFL1 cells. Circ_0008898 was increased in TGF‐β1‐induced HFL1 cells. ASV‐induced impacts were abrogated by circ_0008898 overexpression in TGF‐β1‐induced HFL1 cells. Mechanistically, circ_0008898 competitively bound to miR‐211‐5p to increase the expression of its target HMGB1. MiR‐211‐5p deficiency rescued ASV‐mediated effects in TGF‐β1‐induced HFL1 cells. In addition, HMGB1 overexpression partially overturned circ_0008898 interference‐induced impacts in HFL1 cells upon TGF‐β1 treatment. In conclusion, our work manifested that ASV hindered PF process by mediating the circ_0008898/miR‐211‐5p/HMGB1 network.
Recognizing Alzheimer's disease from perspective of oligodendrocytes: Phenomena or pathogenesis?
Authors: Wang Jingji , Zhen Yilan , Yang Jun , Yang Shaojie , Zhu Guoqi 
Published: March 2024

AbstractBackgroundAccumulation of amyloid beta, tau hyperphosphorylation, and microglia activation are the three highly acknowledged pathological factors of Alzheimer's disease (AD). However, oligodendrocytes (OLs) were also widely investigated in the pathogenesis and treatment for AD.AimsWe aimed to update the regulatory targets of the differentiation and maturation of OLs, and emphasized the key role of OLs in the occurrence and treatment of AD.MethodsThis review first concluded the targets of OL differentiation and maturation with AD pathogenesis, and then advanced the key role of OLs in the pathogenesis of AD based on both clinic and basic experiments. Later, we extensively discussed the possible application of the current progress in the diagnosis and treatment of this complex disease.ResultsMolecules involving in OLs’ differentiation or maturation, including various transcriptional factors, cholesterol homeostasis regulators, and microRNAs could also participate in the pathogenesis of AD. Clinical data point towards the impairment of OLs in AD patients. Basic research further supports the central role of OLs in the regulation of AD pathologies. Additionally, classic drugs, including donepezil, edaravone, fluoxetine, and clemastine demonstrate their potential in remedying OL impairment in AD models, and new therapeutics from the perspective of OLs is constantly being developed.ConclusionsWe believe that OL dysfunction is one important pathogenesis of AD. Factors regulating OLs might be biomarkers for early diagnosis and agents stimulating OLs warrant the development of anti‐AD drugs.
Differences in resting‐state brain activity in first‐episode drug‐naïve major depressive disorder patients with and without suicidal ideation
Authors: Cao Ping , Dai Ke , Liu Xianwei , Hu Jun , Jin Zhuma , Xu Shulan , Ren Fangfang 
Published: March 2024

AbstractDespite altered brain activities being associated with suicidal ideation (SI), the neural correlates of SI in major depressive disorder (MDD) have remained elusive. We enrolled 82 first‐episode drug‐naïve MDD patients including 41 with SI and 41 without SI, as well as 41 healthy controls (HCs). Resting‐state functional and structural MRI data were collected. The measures of fractional amplitude of low‐frequency fluctuation (fALFF) and grey matter volume (GMV) were calculated and compared. Compared with HCs, patients with SI exhibited increased fALFF values in the right rectus gyrus and left medial superior frontal gyrus, middle frontal gyrus and precuneus. Decreased GMV in the right parahippocampal gyrus, insula and middle occipital gyrus and increased GMV in the left superior frontal gyrus were detected in patients with SI. In addition, patients without SI demonstrated increased fALFF values in the right superior frontal gyrus and decreased fALFF values in the right postcentral gyrus. Decreased GMV in the left superior frontal gyrus, right medial superior frontal gyrus, opercular part of inferior frontal gyrus, postcentral gyrus, fusiform gyrus and increased left supplementary motor area, superior occipital gyrus, right anterior cingulate gyrus and superior temporal gyrus were revealed in patients with SI. Moreover, in comparison with patients without SI, increased fALFF values were identified in the left precuneus of patients with SI. However, no significant differences were found in GMV between patients with and without SI. These findings might be helpful for finding neuroimaging markers predicting individual suicide risk and detecting targeted brain regions for effective early interventions.
Integrating singular value decomposition with deep learning for enhanced travel time estimation in multimodal freight transportation networks
Authors: Aljanabi Mohanad R. , Borna Keivan , Ghanbari Shamsollah , Obaid Ahmed J. 
Published: March 2024

AbstractMultimodal freight transport allows switching among various modes of transportation to efficiently utilize transport facilities. A multimodal transport system incorporates geographical scales from global to local. Travel time estimation in a multi‐modal cargo transportation network is essential for enhancing supply chain (SC) and logistics operations. Accurate travel time prediction is of great importance for cargo transportation, as it enables SC participants to increase logistics efficiency and quality. It requires adequate input data, which can be generated. In recent times, the machine learning (ML) algorithm has been well‐suited to resolve complex and nonlinear relationships in the collected tracking data. This study designs a deep learning‐powered travel time estimation in multimodal freight transportation networks (DLTTE‐MFTN) technique. The goal of the DLTTE‐MFTN technique is to estimate the travel time using a hyperparameter‐tuned ensemble learning approach. To achieve this, the DLTTE‐MFTN method initially undergoes data pre‐processing to convert the input raw data into a useful format. In addition, the singular value decomposition (SVD) model can be applied for feature dimensionality reduction in multimodal transport data, considerably improving travel time prediction. Besides, the DLTTE‐MFTN method estimates travel time using an ensemble of three DL approaches including one‐dimensional convolutional neural network (1D‐CNN), stacked autoencoder (SAE) attention, and recurrent neural network (RNN). Finally, the hyperparameter tuning of the DL models takes place using the whale optimization algorithm (WOA). The performance analysis of the DLTTE‐MFTN method takes place using the Kaggle dataset. The experimental results stated that the DLTTE‐MFTN technique attains superior performance over other ML and DL models.
Helicobacter pylori enhances HLA‐C expression in the human gastric adenocarcinoma cells AGS and can protect them from the cytotoxicity of natural killer cells
Authors: Apoorva Etikala , Jacob Rini , Rao Desirazu N. , Kumar Santosh 
Published: March 2024

AbstractHelicobacter pylori (H. pylori) seems to play causative roles in gastric cancers. H. pylori has also been detected in established gastric cancers. How the presence of H. pylori modulates immune response to the cancer is unclear. The cytotoxicity of natural killer (NK) cells, toward infected or malignant cells, is controlled by the repertoire of activating and inhibitory receptors expressed on their surface. Here, we studied H. pylori‐induced changes in the expression of ligands, of activating and inhibitory receptors of NK cells, in the gastric adenocarcinoma AGS cells, and their impacts on NK cell responses. AGS cells lacked or had low surface expression of the class I major histocompatibility complex (MHC‐I) molecules HLA‐E and HLA‐C—ligands of the major NK cell inhibitory receptors NKG2A and killer‐cell Ig‐like receptor (KIR), respectively. However, AGS cells had high surface expression of ligands of activating receptors DNAM‐1 and CD2, and of the adhesion molecules LFA‐1. Consistently, AGS cells were sensitive to killing by NK cells despite the expression of inhibitory KIR on NK cells. Furthermore, H. pylori enhanced HLA‐C surface expression on AGS cells. H. pylori infection enhanced HLA‐C protein synthesis, which could explain H. pylori‐induced HLA‐C surface expression. H. pylori infection enhanced HLA‐C surface expression also in the hepatoma Huh7 and HepG2 cells. Furthermore, H. pylori‐induced HLA‐C surface expression on AGS cells promoted inhibition of NK cells by KIR, and thereby protected AGS cells from NK cell cytotoxicity. These results suggest that H. pylori enhances HLA‐C expression in host cells and protects them from the cytotoxic attack of NK cells expressing HLA‐C‐specific inhibitory receptors.
Switching from combination therapy with entecavir hydrate plus tenofovir alafenamide fumarate to tenofovir alafenamide fumarate monotherapy in patients with chronic hepatitis B based on nucleotide sequences of hepatitis B virus pregenome RNA
Authors: Yamada Shunsuke , Uchida Yoshihito , Kouyama Jun‐Ichi , Naiki Kayoko , Tsuji Shohei , Uemura Hayato , Sugawara Kayoko , Nakayama Nobuaki , Imai Yukinori , Tomiya Tomoaki , Mizuno Suguru , Mochida Satoshi 
Published: March 2024

AbstractAimPatients with chronic hepatitis B virus (HBV) infection experiencing viral breakthrough (BTH) or partial response (PR) during lamivudine (LAM) or entecavir hydrate (ETV) administration often took ETV plus tenofovir alafenamide fumarate (TAF) due to the emergence of a drug‐resistance mutation. However, in patients lacking drug‐resistance mutation against TAF, sufficient antiviral effects may be achievable with TAF monotherapy. We assessed the drug‐resistance profile through nucleotide sequences of HBV pregenome RNA, and subsequently changed to TAF monotherapy from ETV plus TAF.MethodsThis prospective study included 25 patients with serum HBV‐DNA below 20 IU/mL under ETV plus TAF administration. Pregenome RNA nucleotide sequences of HBV in the sera were analyzed using direct sequencing and deep sequencing. ETV was discontinued in patients without rtA194T and rtS106C + rtH126Y + rtD134E + rtL269I quadruple mutations in direct sequencing.ResultsLAM‐PR, LAM‐BTH, ETV‐PR, and ETV‐BTH were observed in 1, 16, 7, and 1 patient(s), respectively. Pregenome RNA nucleotide sequences were analyzable in 20 patients. Among the 12 patients classified as LAM‐BTH, six patients showed rtL180M + rtM204V/I in direct sequencing, and one patient showed minor clones containing rtL180M + rtM204V + A194T in deep sequencing at a frequency of 0.3%. In the six patients classified as ETV‐PR, one patient harbored rtM204I. No clones showing rtS106C + rtH126Y + rtD134E + rtL269I quadruple mutation were detected in deep sequencing. Subsequently, ETV was discontinued, and serum HBV‐DNA remained undetectable up to 48 weeks in all patients.ConclusionPatients receiving ETV plus TAF due to partial response or BTH during initial LAM or ETV administration were able to safely transition to TAF monotherapy based on nucleotide sequences of HBV pregenome RNA in the sera.
Volatile sulfur compounds in asthmatic children and adolescents: A cross‐sectional study in breath and saliva
Authors: Buj‐Acosta Cindy , García‐Sanz Verónica , Hakobyan Lusine , Tarazona‐Álvarez Beatriz , Molins‐Legua Carmen , Campins‐Falcó Pilar , Paredes‐Gallardo Vanessa , Tortajada‐Girbés Miguel 
Published: March 2024

AbstractBackgroundHalitosis in children implies psychosocial repercussions. Risk factors associated with this condition are unclear, and detection methods are inaccurate.AimTo quantify the levels of sulfur‐like compounds in children with asthma and healthy children from a novel validated assay, and to establish the risk factors related to halitosis.DesignOne hundred and twenty‐eight individuals (63 healthy and 65 asthmatic) from 3 to 17 years of age were tested using a passive colorimetric sensor to measure the levels of sulfur‐like compounds in breath and saliva. Information was collected on oral hygiene habits, gingival and dental health, breathing type, and dental malocclusion.ResultsThe mean values of hydrogen sulfide were 4.0 ± 6.8 and 19.7 ± 12.2 ppbv (parts per billion in volume) in the control and asthmatic groups, respectively (p < .001). The presence of higher concentrations of sulfur compounds was significantly associated (p < .05) with the presence of gingival inflammation, tongue coating, dental plaque, mouth breathing, hypomineralization, age, tongue brushing, and the use of dental floss.ConclusionThe level of sulfur in breath and saliva was significantly higher in patients with asthma. These results can serve as a precedent to raise awareness among paediatricians and parents about oral hygiene care in children and adolescents.
Nurses' use of ‘wellness’ supplements during the COVID‐19 pandemic in the United States
Authors: Turner Samantha L. , Beccia Ariel , Feeny Gwenneth , Raffoul Amanda , Jackson Destiny , Sarda Vishnudas , Rich‐Edwards Janet , Chavarro Jorge , Hart Jaime E. , Austin S. Bryn 
Published: March 2024

AbstractAimQuantify disparities and identify correlates and predictors of ‘wellness’ supplement use among nurses during the first year of the pandemic.DesignLongitudinal secondary analysis of Nurses' Health Studies 2 and 3 and Growing Up Today Study data.MethodsSample included 36,518 total participants, 12,044 of which were nurses, who completed surveys during the first year of the COVID‐19 pandemic (April 2020 to April 2021). Analyses were conducted in March 2023. Modified Poisson models were used to estimate disparities in ‘wellness’ supplement use between nurses and non‐healthcare workers and, among nurses only, to quantify associations with workplace‐related predictors (occupational discrimination, PPE access, workplace setting) and psychosocial predictors (depression/anxiety, county‐level COVID‐19 mortality). Models included race/ethnicity, gender identity, age and cohort as covariates.ResultsNurses were significantly more likely to use all types of supplements than non‐healthcare workers. Lacking personal protective equipment and experiencing occupational discrimination were significantly associated with new immune supplement use. Depression increased the risk of using weight loss, energy and immune supplements.ConclusionNurses' disproportionate use of ‘wellness’ supplements during the COVID‐19 pandemic may be related to workplace and psychosocial stressors. Given well‐documented risks of harm from the use of ‘wellness’ supplements, the use of these products by nurses is of concern.Impact‘Wellness’ supplements promoting weight loss, increased energy, boosted immunity and cleansing of organs are omnipresent in today's health‐focused culture, though their use has been associated with harm. This is of added concern among nurses given their risk of COVID‐19 infection at work. Our study highlighted the risk factors associated with use of these products (lacking PPE and experiencing occupational discrimination). Findings support prior research suggesting a need for greater public health policy and education around the use of ‘wellness’ supplements.Reporting MethodSTROBE guidelines were followed throughout manuscript.Patient or Public ContributionNo patient or public contribution was involved.
Nurses' use of an advisory decision support system in ambulance services: A qualitative study
Authors: Fager Cecilia , Rantala Andreas , Svensson Anders , Holmberg Mats , Bremer Anders 
Published: March 2024

AbstractAimTo illuminate from the perspective of nurses in ambulance services the experiences of using a web‐based advisory decision support system to assess care needs and refer patients.DesignInductive and descriptive approaches.MethodThirteen semi‐structured interviews were conducted in the spring of 2020. The data were analysed through the reflexive thematic analysis.ResultsThe Swedish web‐based advisory decision support system (ADSS) was found to strengthen nurses' feelings of security when they assess patients' care needs, promote their competence and professional pride, and help them manage stress. However, the system also generated difficulties for nurses to adjust to the dynamic ambulance team and revealed a discrepancy between their professional roles and responsibilities to refer patients and provide self‐care advice. The nurses thought that the support system facilitated their increased participation and helped them understand patients and significant others by offering transparency in assessment and decision making. Thus, the support system provides nurses with an opportunity to strengthen patients' independence through information and education. However, in the care relationship, nurses worked to overcome patients' expectations.ConclusionNurses using the ADSS increased their security while performing assessments and referrals and found new opportunities to provide information and promote understanding of their decisions. However, nursing care values can be threatened when new support systems are introduced, especially as ambulance services become increasingly protocol‐driven.Implications for Profession and/or Patient CareThese findings have implications for nurses' work environments and help them maintain consistency in making medical assessments and in providing equivalent self‐care advice when referring patients to the different levels of care. The findings will also impact researchers and policymakers who formulate decision support systems.Reporting MethodConsolidated criteria for reporting qualitative research (COREQ).Patient or Public ContributionNone.
SBFI26 induces triple‐negative breast cancer cells ferroptosis via lipid peroxidation
Authors: He Gang , Zhang Yiyuan , Feng Yanjiao , Chen Tangcong , Liu Mei , Zeng Yue , Yin Xiaojing , Qu Shaokui , Huang Lifen , Ke Youqiang , Liang Li , Yan Jun , Liu Wei 
Published: March 2024

AbstractSBFI26, an inhibitor of FABP5, has been shown to suppress the proliferation and metastasis of tumour cells. However, the underlying mechanism by which SBFI26 induces ferroptosis in breast cancer cells remains largely unknown. Three breast cancer cell lines were treated with SBFI26 and CCK‐8 assessed cytotoxicity. Transcriptome was performed on the Illumina platform and verified by qPCR. Western blot evaluated protein levels. Malondialdehyde (MDA), total superoxide dismutase (T‐SOD), Fe, glutathione (GSH) and oxidized glutathione (GSSG) were measured. SBFI26 induced cell death time‐ and dose‐dependent, with a more significant inhibitory effect on MDA‐MB‐231 cells. Fer‐1, GSH and Vitamin C attenuated the effects but not erastin. RNA‐Seq analysis revealed that SBFI26 treatment significantly enriched differentially expressed genes related to ferroptosis. Furthermore, SBFI26 increased intracellular MDA, iron ion, and GSSG levels while decreasing T‐SOD, total glutathione (T‐GSH), and GSH levels.SBFI26 dose‐dependently up‐regulates the expression of HMOX1 and ALOX12 at both gene and protein levels, promoting ferroptosis. Similarly, it significantly increases the expression of SAT1, ALOX5, ALOX15, ALOXE3 and CHAC1 that, promoting ferroptosis while downregulating the NFE2L2 gene and protein that inhibit ferroptosis. SBFI26 leads to cellular accumulation of fatty acids, which triggers excess ferrous ions and subsequent lipid peroxidation for inducing ferroptosis.
Relationship between diabetes treatment status and occupational accidents
Authors: Ogasawara Ayana , Nagata Tomohisa , Odagami Kiminori , Nagata Masako , Adi Nuri Purwito , Mori Koji 
Published: March 2024

ABSTRACTAimsTo evaluate the relationship between diabetes treatment status and occupational accidents.MethodsA cross‐sectional study was conducted using an online questionnaire survey completed in March 2022 among workers aged 20 years or older. The questionnaire included questions about diabetes treatment status and occupational accidents. The association between the treatment status of diabetes and occupational accidents was examined using a logistic regression analysis.ResultsThe study included 25,756 participants with no diabetes, 1,364 participants receiving diabetes treatment, 177 participants who had interrupted diabetes treatment, and 396 participants who needed diabetes treatment but had not seen a doctor. Compared with those with diabetes treatment, the odds ratio of occupational accidents among participants without diabetes was 0.40 (95% CI: 0.32–0.49), the odds ratio among participants with interrupted diabetes treatment was 4.15 (95% CI: 2.86–6.01), and the odds ratio among participants who needed diabetes treatment but had not seen a doctor was 1.25 (95% CI: 0.89–1.77).ConclusionsThis study showed that diabetes treatment status was related to occupational accidents, and it may be important for workers with diabetes to be supported to balance their diabetes management and their work commitments.
The link between the early surgery‐induced inflammatory response and postoperative cognitive dysfunction in older patients
Authors: Brattinga Baukje , Plas Matthijs , Spikman Jacoba M. , Rutgers Abraham , de Haan Jacco J. , van der Wal‐Huisman Hanneke , Absalom Anthony R. , Nieuwenhuijs‐Moeke Gertrude J. , van Munster Barbara C. , de Bock Geertruida H. , van Leeuwen Barbara L. 
Published: March 2024

AbstractBackgroundPostoperative cognitive dysfunction (POCD) is a common complication in older patients with cancer and is associated with decreased quality of life and increased disability and mortality rates. Systemic inflammation resulting in neuroinflammation is considered important in the pathogenesis of POCD. The aim of this study was to explore the association between the early surgery‐induced inflammatory response and POCD within 3 months after surgery in older cancer patients.MethodsPatients ≥65 years in need of surgery for a solid tumor were included in a prospective cohort study. Plasma levels of C‐reactive protein (CRP), interleukin‐1 beta (IL‐1β), IL‐6, IL‐10, and Neutrophil gelatinase‐associated lipocalin (NGAL) were measured perioperatively. Cognitive performance was assessed preoperatively and 3 months after surgery. POCD was defined as a decline in cognitive test scores of ≥25% on ≥2 of five tests within the different cognitive domains of memory, executive functioning, and information processing speed. Logistic regression analysis was performed.ResultsPOCD was observed in 44 (17.7%) of 248 included patients. Age >75, preoperative Mini‐Mental State Examination (MMSE) score ≤26 and major surgery were independent significant predictors for POCD. In multivariate logistic regression analysis, no significant associations were shown between the early surgery‐induced inflammatory response and either POCD or decline within the different cognitive domains.ConclusionsThis study shows that one out of six older patients with cancer developed POCD within 3 months after surgery. The early surgery‐induced inflammatory response was neither associated with POCD, nor with decline in the separate cognitive domains. Further research is necessary for better understanding of the complex etiology of POCD.