bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cholesterol‐regulated stress fiber formation
Authors: Qi Maosong , Liu Yuzhen , Freeman Michael R. , Solomon Keith R. 
Published: February 2009

AbstractDynamic interactions between cellular membranes and the cytoskeleton are known to play major roles in many cellular responses to environmental cues. External signals resulting in proliferation, differentiation, polarization, and motility must be translated from chemical signals into changes of state, often involving the cytoskeleton‐dependent altering of cell shape and redistribution of molecules. Cholesterol, a critical component of eukaryotic cell membranes, performs vital roles in regulating membrane dynamics and function. Here we demonstrate, using mesenchymal and epithelial cell lines, that depletion of membrane cholesterol results in Src kinase‐mediated Rho activation and caveolin phosphorylation, which together collaborate to form stress fibers. These results demonstrate that cholesterol is a critical regulator of membrane‐cytoskeletal dynamics and suggest that altered cholesterol concentrations may result in dramatic changes in cellular responses mediated by the cytoskeleton. J. Cell. Biochem. 106: 1031–1040, 2009. © 2009 Wiley‐Liss, Inc.
Proteomic profiling of distinct clonal populations of bone marrow mesenchymal stem cells
Authors: Mareddy Shobha , Broadbent James , Crawford Ross , Xiao Yin 
Published: February 2009

AbstractMesenchymal stem cells (MSCs) have attracted immense research interest in the field of regenerative medicine due to their ability to be cultured for successive passages and multi‐lineage differentiation. The molecular mechanisms governing MSC self‐renewal and differentiation remain largely unknown. The development of sophisticated techniques, in particular clinical proteomics, has enabled researchers in various fields to identify and characterize cell specific biomarkers for therapeutic purposes. This study seeks to understand the cellular and sub‐cellular processes responsible for the existence of stem cell populations in bone marrow samples by revealing the whole cell proteome of the clonal cultures of bone marrow‐derived MSCs (BMSCs). Protein profiling of the MSC clonal populations was conducted by Two‐Dimensional Liquid Chromatography/Matrix‐Assisted Laser Desorption/Ionisation (MALDI) Mass Spectrometry (MS). A total of 83 proteins were identified with high confidence of which 11 showed differential expression between subpopulations, which included cytoskeletal and structural proteins, calcium binding proteins, cytokinetic proteins, and members of the intermediate filament family. This study generated a proteome reference map of BMSCs from the clonal populations, which will be valuable to better understand the underlying mechanism of BMSC self‐renewal and differentiation. J. Cell. Biochem. 106: 776–786, 2009. © 2009 Wiley‐Liss, Inc.
Two potent transactivation domains in the C‐terminal region of human NANOG mediate transcriptional activation in human embryonic carcinoma cells
Authors: Do Hyun‐Jin , Lee Won‐Young , Lim Hye‐Young , Oh Jong‐Hyun , Kim Dong‐Ku , Kim Jin‐Hoi , Kim Teoan , Kim Jae‐Hwan 
Published: February 2009

AbstractThe core embryonic stem cell transcription factors Oct4, Sox2, and Nanog are expressed in germ cell tumors (GCTs) and have been proposed to play a regulatory role in tumorigenesis. However, little is known about the mechanism of regulation of tumorigenesis by the complicated network of these proteins. Nanog is a novel homeobox‐containing transcription factor that is expressed in pluripotent cells as well as GCTs. To understand the molecular and functional role of human NANOG (hNANOG) in germ cells, mutagenesis of the C‐terminal domain (CD) of hNANOG and transient transfection assays in NCCIT human embryonic carcinoma cells were carried out to identify critical transactivation motifs. We divided the CD into three putative functional subdomains, CD1, tryptophan‐repeat (WR) subdomain, and CD2. WR subdomain and CD2 independently contained transcriptional potential and, in combination, had a synergistic effect on transcriptional activity, while CD1 was transcriptionally inactive. The glutamine (Q) motif in WR subdomain, and multiple acidic residues in CD2 were required for maximal and synergistic transcriptional activation by the hNANOG CD. The results of the current study contribute to a better understanding of the complicated molecular machinery of stem cell transcription factors and their role in unregulated proliferation in germ cell tumorigenesis. J. Cell. Biochem. 106: 1079–1089, 2009. © 2009 Wiley‐Liss, Inc.
Expression of GLUT8 in mouse intestine: Identification of alternative spliced variants
Authors: Romero Amparo , Gomez Olga , Terrado Jose , Mesonero Jose E. 
Published: February 2009

AbstractGLUT8 is a facilitative glucose transporter composed of 10 exons coding for a 477 amino acids protein. It is mainly expressed in the testis, but it has also been studied in a number of tissues such as brain, adipose tissue, and liver. In this work, we have characterized the expression of GLUT8 in the small and large intestine under normal physiological conditions. Protein assay revealed low GLUT8 protein levels in the intestine compared to the testis, with higher levels in the colon than in the small intestine. Immunohistochemistry studies showed an intracellular localization of GLUT8 in enterocytes and colonocytes with a supranuclear distribution next to the apical membrane. GLUT8 immunoreactivity was also detected in the crypt cells. Interestingly, we have identified three additional transcriptional variants in mouse intestine (mGLUT‐SP1, mGLUT8‐SP2, and mGLUT8‐SP3) produced by the deletion of one, two, and four exons, respectively, whereas only the entire mRNA was detected in the testis. Expression of these alternative variants did not have an effect on glucose consumption in 3T3‐L1 cells. Although the specific function of GLUT8 in intestine remains unclear, the alternative splicing of GLUT8 could reflect a mechanism for the regulation of the gene expression in a tissue‐specific manner by targeting GLUT8 mRNA for nonsense‐mediated decay. J. Cell. Biochem. 106: 1068–1078, 2009. © 2009 Wiley‐Liss, Inc.
Time‐dependent density functional calculations on the electronic spectra of the neutral nickel complex [Ni(LISQ)2] (LISQ = 3,5‐di‐tert‐butyl‐o‐diiminobenzosemiquinonate(1−)) and its monoanion and dication
Authors: Bachler Vinzenz 
Published: February 2009

AbstractThe electronic spectrum of the neutral nickel complex [Ni(LISQ)2] (LISQ = 3,5‐di‐tert‐butyl‐o‐diiminobenzosemiquinonate(1−)) and the spectra of its anion and dication have been calculated by means of time‐dependent density functional theory. The electronic ground state of the neutral complex exhibits an open shell singlet diradical character. The mandatory multireference problem for this electronic ground state has been treated approximately by using the unrestricted and spin symmetry broken Kohn‐Sham Slater determinant as the wave function for the noninteracting reference system in the time‐dependent density functional calculations. A reasonable agreement with observed transition energies and band intensities has been achieved. This holds also for the long wavelength transitions that are shown to be of charge transfer type. The charge distributions in the electronic ground state and the corresponding low lying excited states, however, are rather similar. Thus, the known failure of standard time‐dependent density functional theory to describe improperly long range charge transfer transitions is absent in this work. The applied computational scheme might be adequate for calculating electronic spectra of transition metal complexes with noninnocent ligands. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2009
The moderation of an early intervention program for anxiety and depression by specific psychological symptoms
Authors: Cukrowicz Kelly C. , Smith Phillip N. , Hohmeister Holly C. , Joiner Thomas E. 
Published: February 2009

AbstractThe current study examined the influence of a number of psychological factors on the effectiveness of an early intervention program targeting anxiety and depression in a non‐clinical sample of college students. The early intervention program comprised elements of the cognitive‐behavioral analysis system of psychotherapy (McCullough, 2000) delivered in a 2‐hour computer‐based educational program. Participants completed measures of depression, anxiety, and general distress prior to the intervention program and then again 8 weeks later. Additionally, participants were assessed for past major depression, sleep related difficulties, a number of anxiety disorders, and suicide ideation. Moderation of the effectiveness of the early intervention program by these factors depended on the dependent variable of interest, specifically: the effectiveness of the intervention program on symptoms of depression was moderated by insomnia; symptoms of anxiety by past post‐traumatic stress disorder (PTSD) and specific phobia as well as sleep problems related to nightmares; and symptoms of general negative affect by social phobia and suicide ideation. Implications are discussed. © 2009 Wiley Periodicals, Inc. J Clin Psychol 65:1–15, 2009.
Bone morphogenetic protein‐4 induced Rat hepatic progenitor cell (WB‐F344 cell) differentiation toward hepatocyte lineage
Authors: Fan Jianghong , Shen Hong , Dai Qiaomei , Minuk Gerald Y. , Burzynski Frank J. , Gong Yuewen 
Published: February 2009

AbstractHepatic progenitor cells are local stem cells in the liver and they can be differentiated into either hepatocytes or cholangiocytes depending on different stimulations. These stimulations include extracellular growth factors and intracellular transcription factors. Bone morphogenetic protein 4 (BMP4) is a member of transforming growth factor beta (TGF‐β) superfamily and was first identified as growth factor to induce ectopic bone formation from skeletal muscle. Role of BMP4 in the liver is still unclear especially its role in hepatic progenitor cells (HPCs) differentiation. BMP4 was used to stimulate rat HPCs (WB‐F344 cells) and differentiation of WB‐F344 cells was investigated by reverse transcriptase polymerase chain reaction (RT‐PCR) and Western blot analysis. Both adenovirus delivered BMP4 and recombinant BMP4 were able to induce expression of hepatocyte markers such as albumin, TAT‐1, and G6Pase but not cholangiocyte markers such as β4‐integrin and CK19. BMP4 induced differentiation of WB‐F344 cells toward hepatocytes was mediated by increase in phosphorylation of Smad1 and ERK1/2. Moreover, BMP4 also stimulated expression of transcription factor—C/EBP‐α, which involved in differentiation of WB‐F344 cells toward hepatocytes. BMP4 is able to stimulate WB‐F344 cells differentiation toward hepatocyte lineage. J. Cell. Physiol. 220: 72–81, 2009. © 2009 Wiley‐Liss, Inc.
CD247 can bind SHC1, no matter if CD247 is phosphorylated
Authors: Liu Tao , Chen Fangjin , Tang Ning , Feng Jiannan , Zhao Dongsheng , Wei Kaihua , Zhu Yunping , He Fuchu , Liu Shaojun 
Published: February 2009

AbstractOn T cell receptor (TCR) stimulation, src homology 2 domain‐containing transforming protein C1 (SHC1) had been found to bind the tyrosine‐phosphorylated CD247 chain of the receptor via its src homology 2 (SH2) domain, delivering signals that control T cell development and activation. However, how the phosphorylation of CD247 led to the instant binding has not been characterized clearly. To study the binding process in detail, we simulated and compared the interaction processes of the SH2 domain with CD247 and phosphorylated CD247, respectively. Unexpectedly, the simulation revealed that SHC1 can also bind the nonphosphorylated CD247 peptide, which was further validated to be a weak binding by affinity pull‐down experiment. The molecular dynamics (MD) simulation also revealed that the CD247 peptide formed a folding conformation with its Leu209 inserted into the hydrophobic binding pocket in SHC1. And on phosphorylation, it was the electrostatic attraction between the CD247 Tyr(P)206 and the SHC1 Tyr(P)‐binding pocket that destroyed the folding conformation of the nonphosphorylated CD247 and, aided by the electrostatic attraction between SHC1 and the Asp203 of CD247, led to the extended conformation of the phosphorylated CD247 binding to SHC1 strongly. The results suggest that nonphosphorylated CD247 can recruit SHC1 in advance to prepare for the instant needs for SHC1 on TCR stimulation. In view of the ubiquity of phosphorylation in protein interaction regulation, we think this study also exemplified the usefulness of MD in more interactome research involving phosphorylation. Copyright © 2009 John Wiley & Sons, Ltd.
Role of N‐methyl‐D‐aspartate and non‐N‐methyl‐D‐aspartate receptors in the cardiovascular effects of L‐glutamate microinjection into the hypothalamic paraventricular nucleus of unanesthetized rats
Authors: Busnardo Cristiane , Tavares Rodrigo F. , Corrêa Fernando M.A. 
Published: February 2009

AbstractWe report on the cardiovascular effects of L‐glutamate (L‐glu) microinjection into the hypothalamic paraventricular nucleus (PVN) as well as the mechanisms involved in their mediation. L‐glu microinjection into the PVN caused dose‐related pressor and tachycardiac responses in unanesthetized rats. These responses were blocked by intravenous (i.v.) pretreatment with the ganglion blocker pentolinium (PE; 5 mg/kg), suggesting sympathetic mediation. Responses to L‐glu were not affected by local microinjection of the selective non‐NMDA receptor antagonist NBQX (2 nmol) or by local microinjection of the selective NMDA receptor antagonist LY235959 (LY; 2 nmol). However, the tachycardiac response was changed to a bradycardiac response after treatment with LY235959, suggesting that NMDA receptors are involved in the L‐glu heart rate response. Local pretreatment with LY235959 associated with systemic PE or dTyr(CH2)5(Me)AVP (50 μg/kg) respectively potentiated or blocked the response to L‐glu, suggesting that L‐glu responses observed after LY235959 are vasopressin mediated. The increased pressor and bradycardiac responses observed after LY + PE was blocked by subsequent i.v. treatment with the V1‐vasopressin receptor antagonist dTyr(CH2)5(Me)AVP, suggesting vasopressin mediation. The pressor and bradycardiac response to L‐glu microinjection into the PVN observed in animals pretreated with LY + PE was progressively inhibited and even blocked by additional pretreatment with increasing doses of NBQX (2, 10, and 20 nmol) microinjected into the PVN, suggesting its mediation by local non‐NMDA receptors. In conclusion, results suggest the existence of two glutamatergic pressor pathways in the PVN: one sympathetic pathway that is mediated by NMDA receptors and a vasopressinergic pathway that is mediated by non‐NMDA receptors. © 2009 Wiley‐Liss, Inc.
FedEx Ground retools its succession planning and development processes to deliver business results
Authors: D'Alesandro Heather , Crandell Stuart 
Published: February 2009

AbstractA revamped succession planning process at FedEx Ground keeps succession and development decisions clearly focused on the road ahead to ensure the company has its best talent ready for those roles that drive its business strategies and critical initiatives. Changes to the annual talent review process were designed to find and close talent gaps in pivotal leadership roles and competencies, where near‐term talent shortages could pose significant business risks. Prework and the review meeting agenda concentrate the discussion on whether the high‐potential and promotable individuals are filling these pivotal roles and being developed as rapidly as possible. Enabled by a robust technology platform, officers can also devise broader targeted actions across the organization for addressing shared developmental needs related to critical leadership competencies. © 2009 Wiley Periodicals, Inc.
Conducting business in Vietnam: A brief for international managers
Authors: Mai Anh , Bilbard Laurent , Som Ashok 
Published: February 2009

AbstractVietnam joined the World Trade Organization (WTO) to become its 150th member on January 11, 2007. The accession implied Vietnam's willingness to enter the world's common economic playground and its commitments to a more open domestic market, bringing new business and investment opportunities as well as challenges for foreign companies. This article examines the Vietnamese reform process and its recently developed incentives in the country's business and investment environment. It also discusses the business and managerial practices that can help contribute to successful business endeavors in Vietnam. © 2009 Wiley Periodicals, Inc.
Randomized, double‐blind, pilot evaluation of intravenous glutathione in Parkinson's disease
Authors: Hauser Robert A. , Lyons Kelly E. , McClain Terry , Carter Summer , Perlmutter David 
Published: February 2009

AbstractThe objective of this study was to evaluate the safety, tolerability, and preliminary efficacy of intravenous glutathione in Parkinson's disease (PD) patients. This was a randomized, placebo‐controlled, double‐blind, pilot trial in subjects with PD whose motor symptoms were not adequately controlled with their current medication regimen. Subjects were randomly assigned to receive intravenous glutathione 1,400 mg or placebo administered three times a week for 4 weeks. Twenty‐one subjects were randomly assigned, 11 to glutathione and 10 to placebo. One subject who was assigned to glutathione withdrew from the study for personal reasons prior to undergoing any postrandomization efficacy assessments. Glutathione was well tolerated and there were no withdrawals because of adverse events in either group. Reported adverse events were similar in the two groups. There were no significant differences in changes in Unified Parkinson's Disease Rating Scale (UPDRS) scores. Over the 4 weeks of study medication administration, UPDRS ADL + motor scores improved by a mean of 2.8 units more in the glutathione group (P = 0.32), and over the subsequent 8 weeks worsened by a mean of 3.5 units more in the glutathione group (P = 0.54). Glutathione was well tolerated and no safety concerns were identified. Preliminary efficacy data suggest the possibility of a mild symptomatic effect, but this remains to be evaluated in a larger study. © 2009 Movement Disorder Society
Complete assignments of 1H and 13C NMR spectral data for 7,7′‐dihydroarylnaphthalene lignan lactones
Authors: da Silva Rosangela , Carvalho Batista João Henrique , da Silva Maringolo Carine , Donate Paulo Marcos 
Published: February 2009

AbstractIn this article we present a complete 1H and 13C NMR spectral analysis of three 7,7′‐dihydroarylnaphthalene lignan lactones using modern NMR techniques such as COSY, HSQC, HMBC and NOE experiments. Complete assignment and homonuclear hydrogen coupling constant measurements were performed. Copyright © 2009 John Wiley & Sons, Ltd.
Improving non‐contrast‐enhanced steady‐state free precession angiography with compressed sensing
Authors: Çukur Tolga , Lustig Michael , Nishimura Dwight G. 
Published: February 2009

AbstractFlow‐independent angiography offers the ability to produce vessel images without contrast agents. Angiograms are acquired with magnetization‐prepared three‐dimensional balanced steady‐state free precession sequences, where the phase encodes are interleaved and the preparation is repeated before each interleaf. The frequent repetition of the preparation significantly decreases the scan efficiency. The number of excitations can instead be reduced with compressed sensing by exploiting the compressibility of the angiograms. Hence, the phase encodes can be undersampled to save scan time without significantly degrading image quality. These savings can be allotted for preparing the magnetization more often, or alternatively, improving resolution. The enhanced resolution and contrast achieved with the proposed method are demonstrated with lower leg angiograms. Depiction of the vasculature is significantly improved with the increased resolution in the phase‐encode plane and higher blood‐to‐background contrast. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.
Velocity recovery cycles of human muscle action potentials and their sensitivity to ischemia
Authors: Z'Graggen Werner J. , Bostock Hugh 
Published: February 2009

AbstractThis study was undertaken to test whether recovery cycle measurements can provide useful information about the membrane potential of human muscle fibers. Multifiber responses to direct muscle stimulation through needle electrodes were recorded from the brachioradialis of healthy volunteers, and the latency changes measured as conditioning stimuli were applied at interstimulus intervals of 2–1000 ms. In all subjects, the relative refractory period (RRP), which lasted 3.27 ± 0.45 ms (mean ± SD, n = 12), was followed by a phase of supernormality, in which the velocity increased by 9.3 ± 3.4% at 6.1 ± 1.3 ms, and recovered over 1 s. A broad hump of additional supernormality was seen at around 100 ms. Extra conditioning stimuli had little effect on the early supernormality but increased the later component. The two phases of supernormality resembled early and late afterpotentials, attributable respectively to the passive decay of membrane charge and potassium accumulation in the t‐tubules. Five minutes of ischemia progressively prolonged the RRP and reduced supernormality, confirming that these parameters are sensitive to membrane depolarization. Velocity recovery cycles may provide useful information about altered muscle membrane potential and t‐tubule function in muscle disease. Muscle Nerve, 2008
A three‐dimensional integral equation model for calculating poro‐ and thermoelastic stresses induced by cold water injection into a geothermal reservoir
Authors: Zhou X. X. , Ghassemi A. , Cheng A. H.‐D. 
Published: February 2009

AbstractPoro‐mechanical and thermo‐mechanical processes change the fracture aperture and thus affect the water flow pattern in the fracture during the cold water injection into enhanced geothermal systems (EGS). In addition, the stresses generated by these processes contribute to the phenomenon of reservoir seismicity. In this paper, we present a three‐dimensional (3D) partially coupled poro‐thermoelastic model to investigate the poroelastic and thermoelastic effects of cold water injection in EGS. In the model, the lubrication fluid flow and the convective heat transfer in the fracture are modeled by the finite element method, while the pore fluid diffusion and heat conductive transfer in the reservoir matrix are assumed to be 3D and modeled by the boundary integral equation method without the need to discretize the reservoir. The stresses at the fracture surface and in the reservoir matrix are obtained from the numerical model and can be used to assess the variation of in situ stress and induced seismicty with injection/extraction. Application of the model shows that rock cooling induces large tensile stresses and increases fracture conductivity, whereas the rock dilation caused by fluid leakoff decreases fracture aperture and increases compressive total stresses around the injection zone. However, increases in pore pressure reduce the effective stresses and can contribute to rock failure, fracture slip, and microseismic activity. Copyright © 2009 John Wiley & Sons, Ltd.
Predicting who will benefit from treatment
Authors: Herbison Peter 
Published: February 2009

AbstractIt is often of interest whether some subgroup either responds better, or does not respond at all, to a treatment. To decide this requires a subgroup analysis. The best way to do this is as part of an individual patient data meta‐analysis. To facilitate these there is a need for repositories for individual data from randomized controlled trials. Neurourol. Urodynam. 28:168–169, 2009. © 2009 Wiley‐Liss, Inc.