bluuebunny/crossref_metadata_2025_split huggingface dataset:
Giants among Cnidaria: Large Nuclear Genomes and Rearranged Mitochondrial Genomes in Siphonophores
Authors: Ahuja Namrata , Cao Xuwen , Schultz Darrin T , Picciani Natasha , Lord Arianna , Shao Shengyuan , Jia Kejue , Burdick David R , Haddock Steven H D , Li Yuanning , Dunn Casey W 
Published: March 2024

Abstract
Siphonophores (Cnidaria: Hydrozoa) are abundant predators found throughout the ocean and are important constituents of the global zooplankton community. They range in length from a few centimeters to tens of meters. They are gelatinous, fragile, and difficult to collect, so many aspects of the biology of these roughly 200 species remain poorly understood. To survey siphonophore genome diversity, we performed Illumina sequencing of 32 species sampled broadly across the phylogeny. Sequencing depth was sufficient to estimate nuclear genome size from k-mer spectra in six specimens, ranging from 0.7 to 2.3 Gb, with heterozygosity estimates between 0.69% and 2.32%. Incremental k-mer counting indicates k-mer peaks can be absent with nearly 20× read coverage, suggesting minimum genome sizes range from 1.4 to 5.6 Gb in the 25 samples without peaks in the k-mer spectra. This work confirms most siphonophore nuclear genomes are large relative to the genomes of other cnidarians, but also identifies several with reduced size that are tractable targets for future siphonophore nuclear genome assembly projects. We also assembled complete mitochondrial genomes for 33 specimens from these new data, indicating a conserved gene order shared among nonsiphonophore hydrozoans, Cystonectae, and some Physonectae, revealing the ancestral mitochondrial gene order of siphonophores. Our results also suggest extensive rearrangement of mitochondrial genomes within other Physonectae and in Calycophorae. Though siphonophores comprise a small fraction of cnidarian species, this survey greatly expands our understanding of cnidarian genome diversity. This study further illustrates both the importance of deep phylogenetic sampling and the utility of k-mer–based genome skimming in understanding the genomic diversity of a clade.
Domestic violence: prevention past due
Authors: Chang Debbie I 
Published: March 2024

Abstract
In May 2023, the White House released the National Plan to End Gender-Based Violence, which includes intimate partner or domestic violence (DV). Based on 20 years of experience in California, this commentary provides detailed examples of 2 DV prevention strategies: interrupting intergenerational transmission and addressing macrolevel drivers. Family-strengthening approaches to prevention and justice and increasing economic security are key. Insight into regional policies and programs can inform implementation of the national plan and DV prevention in other states and localities.
Front-line guardian of the status quo: Japan under the Kishida government
Authors: Singh Bhubhindar 
Published: March 2024

Abstract
Prime Minister Fumio Kishida and his government have implemented bold defence, diplomatic and economic policies against the challenges to the status quo order posed by Russia's invasion of Ukraine and increased Chinese assertiveness. What do these policies unveil about Japan's strategy and response to the transformed strategic environment? This article argues that the Kishida government has upgraded Japan to a front-line guardian of the status quo order. To achieve its front-line role, the Kishida government has consolidated Japan's deterrence policy, namely focusing on two main pillars—military and rules. To ensure Japan's frontline role is sustainable, this policy paper makes three recommendations: Japan must develop strategic clarity and clear operational plans on how to respond to a regional contingency, such as the Taiwan Strait; Japan must resolve domestic problems that prevent the increase in defence spending to 2 per cent in the period to 2027. Japan's role as a front-line state to protect the status quo order is very much dependent on the availability of resources that would be provided by this increase in defence spending; Japan should re-energize its efforts towards east Asia through a sustained diplomatic, economic and security means. This is important in securing support for Japan's preferred vision of regional and global order from not only like-minded partners and friends in east Asia, but also China. Japan's approach should underscore how the extant status quo order benefits all states, including China, especially in terms of spreading economic prosperity, development and connectivity.
Gender in European Union Asylum Law: The Istanbul Convention as a Game Changer?
Authors: Warin Catherine 
Published: March 2024

Abstract
The European Union (EU) has historically been a proactive player in advancing equality between women and men, and fighting gender-based discrimination. The past two decades have also seen the EU becoming a major actor in asylum law, with several EU secondary law instruments and a large amount of case law in EU Member States relating to the application of the 1951 Refugee Convention. Yet, these two areas of EU legislation – gender equality and asylum – have yet to become consistently connected. Similarly, judgments of the Court of Justice of the European Union addressing gender-related elements of asylum cases are scarce. Could the Council of Europe Convention on Preventing and Combating Violence against Women and Domestic Violence (Istanbul Convention) help bridge the gap? The Istanbul Convention identifies gender-based violence as an extreme form of discrimination and contains a whole chapter dedicated to women in the context of migration. This article sheds light on the yet-to-be-realized potential of the Istanbul Convention to amplify the protective power of the Refugee Convention in the EU. While at present neither the Common European Asylum System, nor its new iteration in the New Pact on Migration and Asylum, fully meets the standards of the Istanbul Convention, it may be expected that the EU’s accession to the treaty will further encourage a gender-sensitive approach in EU asylum law. The Istanbul Convention may well be a game changer for the protection of female asylum seekers, and possibly also for asylum seekers with other gender identities.
Impact of the phenotypic expression of temocillin resistance in Escherichia coli on temocillin efficacy in a murine peritonitis model
Authors: Mallart Elise , Guerin François , Amoura Ariane , Le Scouarnec Matthieu , Hamon Antoine , El Meouche Imane , Chau Françoise , Lefort Agnès , Fantin Bruno , Cattoir Vincent , de Lastours Victoire 
Published: March 2024

Abstract

Background
Temocillin is a narrow spectrum β-lactam active against MDR Enterobacterales. Mechanisms of acquired resistance to temocillin are poorly understood. We analysed resistance mechanisms in clinical isolates of Escherichia coli and evaluated their impact on temocillin efficacy in vitro and in a murine peritonitis model.

Methods
Two sets of isogenic clinical E. coli strains were studied: a susceptible isolate (MLTEM16S) and its resistant derivative, MLTEM16R (mutation in nmpC porin gene); and temocillin-resistant derivatives of E. coli CFT073: CFT-ΔnmpC (nmpC deletion), CFTbaeS-TP and CFTbaeS-AP (two different mutations in the baeS efflux-pump gene).
Fitness cost, time–kill curves and phenotypic expression of resistance were determined. Temocillin efficacy was assessed in a murine peritonitis model.

Results
MICs of temocillin were 16 and 64 mg/L for MLTEM16S and MLTEM16R, respectively, and 8, 128, 256 and 256 mg/L for E. coli-CFT073, CFT-ΔnmpC, CFTbaeS-TP and CFTbaeS-AP, respectively. No fitness cost of resistance was evidenced. All resistant strains showed heteroresistant profiles, except for CFTbaeS-AP, which displayed a homogeneous pattern. In vitro, temocillin was bactericidal against MLTEM16R, CFT-ΔnmpC, CFTbaeS-TP and CFTbaeS-AP at 128, 256, 512 and 512 mg/L, respectively.
In vivo, temocillin was as effective as cefotaxime against MLTEM16R, CFT-ΔnmpC and CFTbaeS-TP, but inefficient against CFTbaeS-AP (100% mortality).

Conclusions
Heteroresistant NmpC porin alteration and active efflux modification do not influence temocillin efficacy despite high MIC values, unfavourable pharmacokinetic/pharmacodynamic conditions and the absence of fitness cost, whereas homogeneously expressed BaeS efflux pump alteration yielding similar MICs leads to temocillin inefficacy. MIC as sole predictor of temocillin efficacy should be used with caution.
Evolution of wing shape in geometrid moths: phylogenetic effects dominate over ecology
Authors: Ude Kadri , Õunap Erki , Kaasik Ants , Davis Robert B , Javoiš Juhan , Nedumpally Vineesh , Foerster Stenio I A , Tammaru Toomas 
Published: March 2024

Abstract
Locomotory performance is an important determinant of fitness in most animals, including flying insects. Strong selective pressures on wing morphology are therefore expected. Previous studies on wing shape in Lepidoptera have found some support for hypotheses relating wing shape to environment-specific selective pressures on aerodynamic performance. Here, we present a phylogenetic comparative study on wing shape in the lepidopteran family Geometridae, covering 374 species of the northern European fauna. We focused on 11 wing traits including aspect ratio, wing roundness, and the pointedness of the apex, as well as the ratio of forewing and hindwing areas. All measures were taken from images available on the internet, using a combination of tools available in Fiji software and R. We found that wing shape demonstrates a phylogenetically conservative pattern of evolution in Geometridae, showing similar or stronger phylogenetic signal than many of its potential predictors. Several wing traits showed statistically significant associations with predictors such as body size, phenology, and preference for forest habitats. Overall, however, all of these associations remained notably weak, with no wing shape being excluded for any value of the predictors, including body size. We conclude that, in geometrids, wing traits do not readily respond to selective pressures optimizing aerodynamic performance of the moths in different environments. Selection on wing shape may nevertheless operate through other functions of the wings, with the effectiveness of crypsis at rest being a promising candidate for further studies.
The invasive ambrosia beetle, Gnathotrichus materiarius (Coleoptera: Curculionidae), in Central Europe
Authors: Fiala Tomáš , Holuša Jaroslav , Resnerová Karolina , Foit Jiří , Lakatos Ferenc , Mazur Andrzej , Procházka Jiří , Witkowski Radoslaw , Pyszko Petr , Holzschuh Carolus 
Published: March 2024

Abstract
Gnathotrichus materiarius Fitch, 1858 is an invasive bark beetle that colonizes conifers and has spread widely in Europe. The beetle was introduced from North America to Western Europe, where it was first detected in France in 1933. In countries of Western and Northern Europe, the first detections were found at ports or airports, which probably served as sources of further spread. Gnathotrichus materiarius spread eastward to the Czech Republic and other countries in Central Europe from Germany and Poland and spread northward from Italy to Slovenia. The presence of wilting spruces, outbreaks dominated by Ips typographus Linnaeus, 1758, and the subsequent transport of timber have probably accelerated the spread of G. materiarius. It is possible that G. materiarius was imported with timber to Austria, the Czech Republic, and Poland. Although G. materiarius has been present in Europe for almost 100 yr, and even though its host trees include Picea and Pinus spp., which are abundant in Central European forests, no significant damage caused by this beetle has been detected or reported. Gnathotrichus materiarius is a typical secondary pest in that it multiplies on decaying trees or trees already infested and killed by other bark beetle species. It has 2 generations a year in Central Europe. The beetles of G. materiarius occur the whole year, but the flight of adults starts in early May and the offspring beetles emerge in mid-July. The adults of F2 generation overwinter in wood. The best method for monitoring and detecting the presence of G. materiarius is the use of ethanol-baited traps.
Surgical treatment of patients with chronic rupture of the pectoralis major muscle tendon. Prospective comparative study with 2 years of follow-up
Authors: de Castro Pochini Alberto , Ejnisman Benno , Andreoli Carlos V , Yamada Andre F , Godoy Ivan R B , Cohen Moises , Seixas Maria T , Belangero Paulo S , Hipolide Debora C 
Published: March 2024

Abstract
To compare outcomes between autologous fascia lata and autologous hamstring grafts for chronic pectoralis major muscle (PMM) rupture repair, and perform histological, and imaging analyses. Forty male patients with chronic PMM ruptures (time since injury ranging from &gt;3 months to 5 years) and a mean age of 37.3 years (SD = 9.7 years) were evaluated. One group (20 patients) received an autologous semitendinosus graft, and another group (20 patients) received an autologous fascia lata graft for PMM reconstruction. These patients with fascia lata grafts by Bak 2criterium 60% of the patients presented excellent results, 20% presented good results, 15% presented fair results, and 5% presented poor results. In the hamstring group 65% of the patients presented excellent results, 30% presented good results, and 5% presented fair results. In this comparative study, no difference was observed regarding the functional result, image, and histology between groups.
Distal rupture of the biceps brachii tendon in a female mixed martial arts athlete: a case report
Authors: Bernardo Franciele S , Dada Neto Francisco , Lima Ewerton B D S , Lara Paulo H S , Pochini Alberto D C , Andreoli Carlos V , Belangero Paulo S , Ejnisman Benno 
Published: March 2024

Abstract
The distal rupture of the biceps brachii muscle tendon (DBT) accounts for 3% of biceps ruptures. Diagnosis typically relies on high clinical suspicion and complementary imaging studies, with &gt;90% of cases documented in males between the fourth and sixth decades of life. Reports of DBT ruptures in females are scarce, mostly involving partial and degenerative injuries. Here, we present an unprecedented case of a 28-year-old female professional mixed martial arts athlete with a total traumatic DBT rupture. The athlete underwent surgical repair using anchor reattachment technique. No complications were observed, and the athlete showed satisfactory outcomes, being cleared for physiotherapy after 2 weeks and returning to sports after a 3-month postoperative period.
A rare case of eosinophilic jejunitis: diagnosis and management strategies
Authors: Aaboudech T Y , Zouaidia F , Znati K , Bernoussi Z , Jahid A 
Published: March 2024

Abstract
Eosinophilic gastroenteritis is a rare disease with an unknown cause, which can manifest independently or as part of a hyper-eosinophilic syndrome. The severity of the condition depends on the extent of eosinophilic infiltration and damage to the digestive tract. Diagnosis relies on histological examination, which reveals a significant presence of eosinophilic polymorphonuclear leukocytes in the digestive wall. The authors present a new case of eosinophilic gastroenteritis in a 28-year-old patient who exhibited obstructive symptoms but lacked peripheral eosinophilia. Esophagogastroduodenoscopy showed no abnormalities, but barium transit imaging revealed gastro-duodeno-jejunal dilation upstream of a tight jejunal stenosis. Surgical examination of the affected area confirmed a diffuse and transparietal eosinophilic infiltrate, with no evidence of parasitic or granulomatous lesions. Fortunately, the patient had a swift recovery following surgery. Biopsies conducted at other locations, including the gastric, hepatic, and medullary levels, produced negative results, indicating the localized nature of the condition.
Traumatic air embolism; a case report and literature review of this rare complication of trauma
Authors: Morreau Jonty H 
Published: March 2024

Abstract
Trauma management forms a significant component of any health system. It can affect any body system, and as such knowledge of the wide possible presentations and sequalae are critical. Systemic air embolism (SAE) is a rare presentation in trauma, though it can be associated with significant morbidity and mortality. We present the case of a 23-year-old gentleman with isolated penetrating trauma to the chest who developed widespread neurological insult as a result of SAE, and review historical and proposed contemporary management of SAE.
There is a place for every animal, but not in my back yard: a survey on attitudes towards urban animals and where people want them to live
Authors: Sweet Fabio S T , Mimet Anne , Shumon Md Noor Ullah , Schirra Leonie P , Schäffler Julia , Haubitz Sophia C , Noack Peter , Hauck Thomas E , Weisser Wolfgang W 
Published: March 2024

Abstract
Animals are a constant presence in urban environments. While there is a handful of studies that have addressed which urban animals people like, there is little knowledge on where in the city people want them to be. There is the risk of a misalignment between human urban inhabitants’ specific desires for more nature in cities and the results of urban renaturing initiatives. We conducted an online survey on inhabitants of Munich (Germany) to investigate their attitudes towards 32 different urban animals and where they want them to occur. These places ranged from their own home or garden, to different places within the city, and to outside of the city. In total, 10 000 flyers were distributed in houses surrounding 40 city squares, and 305 people participated. We found that people rather placed animals further away than close to home. There were clear differences between different animal species in where they were placed by people, from species being placed close to home, to species wanted only outside the city. There were also clear differences in attitude towards the different animals, and participants on averaged placed animals closer to home if they liked them more. People have clear preferences for the placing of different animals in an urban context. Knowledge on these preferences can help to design targeted conservation actions and environmental education programs.
A tomato B-box protein regulates plant development and fruit quality through the interaction with PIF4, HY5, and RIN transcription factors
Authors: Shiose Lumi , Moreira Juliene dos Reis , Lira Bruno Silvestre , Ponciano Gabriel , Gómez-Ocampo Gabriel , Wu Raquel Tsu Ay , dos Santos Júnior José Laurindo , Ntelkis Nikolaos , Clicque Elke , Oliveira Maria José , Lubini Greice , Floh Eny Iochevet Segal , Botto Javier Francisco , Ferreira Marcelo José Pena , Goossens Alain , Freschi Luciano , Rossi Magdalena 
Published: March 2024

Abstract
During the last decade, knowledge about BBX proteins has greatly increased. Genome-wide studies identified the BBX gene family in several ornamental, industry, and food crops; however, reports regarding the role of these genes as regulators of agronomically important traits are scarce. Here, by phenotyping a knockout mutant, we performed a comprehensive functional characterization of the tomato locus Solyc12g089240, hereafter called SlBBX20. The data revealed the encoded protein as a positive regulator of light signaling affecting several physiological processes during the life span of plants. Through inhibition of PHYTOCHROME INTERACTING FACTOR 4 (SlPIF4)–auxin crosstalk, SlBBX20 regulates photomorphogenesis. Later in development, it controls the balance between cell division and expansion to guarantee correct vegetative and reproductive development. In fruits, SlBBX20 is transcriptionally induced by the master transcription factor RIPENING INHIBITOR (SlRIN) and, together with ELONGATED HYPOCOTYL 5 (SlHY5), up-regulates flavonoid biosynthetic genes. Finally, SlBBX20 promotes the accumulation of steroidal glycoalkaloids and attenuates Botrytis cinerea infection. This work clearly demonstrates that BBX proteins are multilayer regulators of plant physiology because they affect not only multiple processes during plant development but they also regulate other genes at the transcriptional and post-translational levels.
Public versus Private Care in the Military Health System: Evidence From Low Back Pain Patients
Authors: Leggett Christopher G , Schmidt Rachel O , Skinner Jonathan , Lurie Jon D , Luan William Patrick 
Published: March 2024

ABSTRACT

Introduction
There is a longstanding debate about whether health care is more efficiently provided by the public or private sector. The debate is particularly relevant to the Military Health System (MHS), which delivers care through a combination of publicly funded federal facilities and privately contracted providers. This study compares outcomes, treatments, and costs for MHS patients obtaining care for low back pain (LBP) from public versus private providers.

Materials and Methods
A retrospective cohort study was completed using TRICARE Prime claims data from April 2015 to December 2018. The cohort was identified using International Classification of Diseases Ninth Revision and Tenth Revision diagnostic codes and then followed for 12 months after the index diagnosis to assess treatments, outcomes, and costs. Claims were classified as originating from either public or private providers. The primary outcome measure was resolution of LBP, defined as an absence of LBP diagnoses during the 6-to-12-month window following the index event. Instrumental variable models were used to assess the impact of care setting (i.e., private versus public), conditioning on the covariates. A regional measure of the fraction of private care was used as an instrument.

Results
Resolution of LBP was achieved for 79.7% of 144,866 patients in the cohort. No significant association was found between resolution of LBP and fraction of privately provided care. Higher fraction of private care was associated with a greater likelihood of opioid treatments (odds ratio, 1.22; 95% CI, 1.02-1.46) and a lower likelihood of benzodiazepine (odds ratio, 0.56; 95% CI, 0.45-0.70) and physical therapy (odds ratio 0.55; 95% CI, 0.42-0.74) treatments; manual therapy was not significantly associated with the fraction of private care. There was a significant negative association between the fraction of private care and cost (coefficient −0.27; 95% CI, −0.44, −0.10).

Conclusion
This study found that privately provided care was associated with significantly higher opioid prescribing, less use of benzodiazepines and physical therapy, and lower costs. No systematic differences in outcomes (as measured by resolved cases) were identified. The findings suggest that publicly funded health care within the MHS context can attain quality comparable to privately provided care, although differences in treatment choices and costs point to possibilities for improved care within both systems.