bluuebunny/crossref_metadata_2025_split huggingface dataset:
T cell nature of exocytic and dermal lymphoid cells in atrophic parapsoriasis demonstrated by monoclonal Leu 1 and affinity isolated antibodies
Authors: Mcmillan Euan M. , Wasik Rose , Martin Douglas , Everett Mark Allen 
Published: April 2006

Tissues from atrophic large plaque parapsoriasis were examined using the indirect immunoperoxidase technique with a monoclonal T cell antibody as primary antibody, and affinity isolated peroxidase conjugated goat anti‐mouse IgG as second stage antibody.The “T” cell nature of the dermal infiltrate and of single exocytic epidermal lymphocytes was demonstrated. The results obtained suggest that the method utilized will be useful for the demonstration of lymphoid cell subpopulations in a variety of cutaneous disorders in which a lymphocytic infiltrate forms a significant component of the histopathology observed. This technique also has the potential of providing information on the topographic localization and differentiation of lymphocytes within the various levels of the skin. Certain technical manipulations which may result in an improvement in the quality of results obtained are also discussed.
Blue rubber‐bleb nevus syndrome with Masson's vegetant intravascular hemangioendothelioma
Authors: Kumakiri Masanobu , Fukaya Tohru , Miura Yusho 
Published: April 2006

A 14‐year‐old Japanese male showed numerous small cutaneous hemangiomas and severe anemia produced by internal hemorrhages from intestinal hemangiomas. Histological studies revealed ectatic vessels lined by a single layer of endothelial cells. Six hemangiomas, with fibrous walls of variable thickness, showed characteristics of vegetant intravascular hemangioendothelioma (Masson) in an organizing thrombus. Electron microscopy confirmed that the dilated cavities, were lined by a layer of flattened endothelial cells, surrounded by a few pericytes and/or smooth muscle cells. No appreciable abnormalities were found in the blood vessels located in the clinically normal skin.A few amyloid deposits in the subepidermal connective tissue were also observed.
Tumor promotion by regenerative epidermal hyperplasia in mouse skin
Authors: Argyris Thomas S. 
Published: April 2006

Chemically induced epidermal carcinogenesis is usually divided into two stages: initiation, which involves the conversion of epidermal cells into latent neoplastic cells; and promotion, which allows the expression of this neoplastic change. One of the characteristics of promotion is the transformation of the normal epidermis into a hyperplastic epidermis.One of the unanswered questions about epidermal tumor promotion is whether the epidermal hyperplasia that characterizes promoted skin is simply a regenerative epidermal hyperplasia resulting from damage produced by the promoter. The opinion currently held is that the epidermal hyperplasia produced by tumor promoters is not simply a regenerative epidermal hyperplasia and possesses characteristics which a regenerative hyperplasia does not have, enabling it to evolve into an epidermal neoplasm.The purpose of this review is to present recent evidence which strongly suggests that promoter induced hyperplasia is a regenerative hyperplasia. Two principal lines of evidence arc reviewed. The first demonstrates that an epidermal regenerative hyperplasia repeatedly produced by wounding or abrasion can promote epidermal carcinogenesis in the initiated skin of mice. The second line of evidence demonstrates that the epidermal hyperplasia produced by the application of 12‐O‐tetradccanoyl‐phorbol‐13‐acetate (TPA), the most powerful and widely used promoter of skin carcinogenesis, is preceded by damage to the epidermis. This strongly suggests that the epidermal hyperplasia which ensues is a regenerative hyperplasia.The review concludes that during epidermal tumor promotion there is a chronic regenerative epidermal hyperplasia, and that there is no evidence for any other necessary factors during tumor promotion in appropriately initiated mouse skin.
The nature of melanoma. A critical review
Authors: McGovern V. J. 
Published: April 2006

The main object of this review was to examine the various histogenetic types of melanoma in order to determine their nature. Nodular melanoma and superficial spreading melanoma differ in the more rapid growth of the former. For tumors of equal depth of invasion in patients of the same sex, the prognoses are similar. Clinical features with prognostic significance are sex, age, and site of the lesion. Women have a decided superiority in survival up to the age of about 50 years when their superiority declines. Survival rates for men also decline after the age of 50 years but to a lesser degree. Melanomas of the extremities have a better prognosis than melanomas of the axial regions. The histological feature of most prognostic significance is depth of invasion (thickness). Ulceration is partly bound to thickness of the lesion, but has an augmentative effect of its own which is related to rate of growth. Thin lesions with or without regression are often associated with meta‐stases.Melanomas arising in Hutchinson's melanotic freckle have a better prognosis than nodular or superficial spreading melanoma but there has not been any series large enough for definitive markers with prognostic significance lo be determined. A similar state pertains in palmar, plantar and subungual melanomas.The initial surgical approach in nodular and superficial melanoma should be based upon the thickness of the tumour, site of the tumour, and sex of the patient. The current classification of malignant melanoma is unsatisfactory. Melanoma arising in Hutchinson's melanotic freckle seems to be a distinct entity. Melanomas of other histogenetic types would be best classified according to site.
A light and electron microscopic study of an atypical calcifying odontogenic tumor containing “amyloid”
Authors: Mohamed A. H. , Waterhouse J. P. 
Published: April 2006

Abstract. The light and electron microscopic appearances of an atypical calcifying odontogenic mandibular tumor containing “amyloid” arc presented. In a part of the tumor, PAS‐reactive hyalin eosinophilic material which showed green dichroism by polarized light after Congo Red staining, and also hematoxylinophilic mineralized portions were found. By electron microscopy the hyalin material revealed a fibrillar structure. The fibrils had a median diameter of 100 Å. and were short and curved with some apparent outer modularity. They differed morphologically from other unidentified extracellular and intracellular densely packed fibrils of about 85 Å in diameter, from collagen fibers and from a small number of elastic fibers found in the connective tissue. Apparent calcification had occurred in aggregates of the 100 Å fibrils to produce‐ a linear and angulated electron‐dense front. Calcification within epithelial cells of the tumor, and calcification in irregular foci around collagen fibers were also found.Quite different histopathological appearances were noted by light microscopy in different parts of the tumor. An anterior region contained numerous islands with large areas of odontogenic epithelium with peripheral ameloblast‐like cells and also solid sheets of cuboidal epithelial cells resembling in some ways those of the calcifying epithelial odontogenic tumor.The upper or posterior region of the tumor contained marked cavernous hemangiomatous elements separated by a loose and often myxoid stroma. Metaplastic cartilage formation had occurred in one part of it. The third molar tooth was located near the coronoid notch. The tumor, although not precisely classified, appeared to be an atypical ameloblastic fibro‐odontoma.
Leukocyte mediated cytotoxicity of chemically induced rat salivary gland neoplasms
Authors: Brown Ashley M. , Frankel Arthur 
Published: April 2006

Abstract. Chemically induced tumors of the rat submaxillary glands were tested for the presence of tumor antigens by in vitro leukocyte mediated cytotoxicity assays. Since it has been reported that the saliva of humans with oral cancer contains markedly elevated levels of secretory antibody, the rats' saliva specimens were also tested for blocking and/or potentiating “antibody” activity.The results indicate that saliva from tumor‐bearing animals potentiated tumor cell killing if either the tumor cells or leukocytes were pretreated. The effect was much more marked when tumor cells were pretreated. Furthermore, the addition of saliva to tumor cells imparted an immunologic cross reactivity which was not present with untreated tumor cells. Control saliva occasionally produced a similar effect, but not as consistently or with the potency of saliva from tumor‐bearing animals. Heat inactivation did not abrogate this potentiation.It is inferred that this may indicate a new system of immunologic surveillance, i.e. arming or potentiation of leukocytes by soluble tumor antigens in glandular secretions.
The effect of oral physiotherapy on Dilantin gingival hyperplasia
Authors: King Don Allan , Hawes Roland R. , Bibby Basil G. 
Published: April 2006

Abstract.Gingival hyperplasia was studied in 13 boys with epilepsy living in a state hospital. Boys were selected on the basis of having gingival hyperplasia, having all teeth between cuspids (upper and lower), having no occlusal abnormality and being cooperative. After gingivectomy, regrowth of gingiva was compared around lateral incisors on one side of the mouth having operator‐assisted oral hygiene with that around lateral incisors on the other side of the mouth without operator‐assisted oral hygiene. Regrowth of tissue was documented by precise photogrammetry. Oral hygiene, gingival inflammation and crevicular fluid were monitored. Less inflammation, less crevicular fluid and less regrowth of gingival tissues occurred around teeth subjected to good oral hygiene. Precise periodic photographic documentation of the clinical status of patients during studies such as this is considered very valuable.
Collagenase Studies in bones of guinea pigs*
Authors: Heard Sam Bryan 
Published: April 2006

Abstract.A specific collagenase has been demonstrated in culture fluids of guinea pig bones. Based on the acrylamide gel eleclrophoretic pattern, the mechanism of action of guinea pig bone collagenase appears similar to other known vertebrate collagenases. Two peaks of enzymatic activity were found upon assay of fractions from gel chromatography, corresponding to molecular weights in the range of 137,000 daltons and 29,000 daltons. The enzyme is inhibited by cysteine and EDTA. Alpha2‐macroglobulin is a much stronger inhibitor of the collagenase than alpha1‐antitrypsin. Activation studies suggested the presence of a proenzyme to guinea pig bone collagenase that was converted to an active enzyme by proteases present in the culture fluid.
Histopathologic evaluation of the effects of four calcium hydroxide liners on monkey pulps
Authors: Heys D. R. , Heys R. J. , Cox C. F. , Avery J. K. 
Published: April 2006

Abstract.Pulpal response of four calcium hydroxide liners, MPC 10®, MPC 12®, Dycal® and Pulpdent® were tested on primary and permanent teeth with zinc oxide and eugenol (ZOE) and silicate as controls. Responses of the pulps were evaluated in Rhesus monkeys, utilizing Class V cavity preparations at 3 days, 5 and 8 weeks. An equivalent number of anterior and posterior teeth were studied for all compounds. The Ca(OH)2 liners, zinc oxide and eugenol (ZOE) and silicate controls were placed in 80 primary and 80 permanent teeth. Following perfusions the teeth were prepared utilizing routine histological procedures. The 3 day response of the calcium hydroxides was moderate with some disruption in the odontoblasts, vacuolization and mild inflammation underlying the cavity except Pulpdent which was more severe. At 5 weeks a decrease in inflammatory response and the formation of reparative dentin was similar for all calcium hydroxides tested at this time period. At 8 weeks more reparative dentin was noted with slight to moderate pulpal responses. At all time periods ZOE produced the least pulpal response while silicate produced the most severe response at 5 and 8 weeks.This study reports the biological responses of four calcium hydroxide compounds used as cavity liners in non‐exposures in a series of primary and permanent teeth of monkeys using ZOE and silicate as controls. Responses to the four Ca(OH)2 compounds were moderate for all the experimental compounds except Pulpdent which was more severe at the early time period tested. ZOE produced a milder and silicate a severe response at all periods. All of these compounds were placed by random selection in anterior and posterior teeth of both arches and five teeth were evaluated in both primary and permanent teeth at 3 days, 5 and 8 weeks.
Changes in granular endoplasmic reticulum induced in cultured human monocytes by colony stimulating factor
Authors: Mohammad Abdel Rahim 
Published: April 2006

Abstract.The supernatant portion of cultured human monocytes produces a biological factor known as colony stimulating factor. The addition of culture fluid containing colony stimulating factor to cultured human monocytes produced morphologic changes in the granular endoplasmic reticulum. Granular endoplasmic reticulum changed its appearance from long slender cisternae to a concentric pattern (whorls) averaging seven rows per structure 120 min following the addition of culture fluids containing colony stimulating factor.
Atrophic lesions of the tongue: A prevalence study among 175 diabetic patients
Authors: Farman Allan G. 
Published: April 2006

Abstract.Central papillary atrophy and other anomalies of the tongue were studied among 175 diabetic outpatients attending the Tygerberg Hospital. Atrophic lesions of the tongue were found in 26.9 % of the patients and 61.7 % of these lesions were central papillary atrophy. The degree of control of the diabetes as measured by plasma glucose and urine analyses was not related to the frequency of central papillary atrophy. The prevalence of central papillary atrophy in diabetics was found to be much higher than that of central papillary atrophy and median rhomboid glossitis found in previous investigations among other populations. It is suggested that patients with central papillary atrophy should be screened to rule out diabetes mellitus as the underlying cause. Double central fissures, producing well demarcated triangular areas with their bases at the “sulcus terminalis”, were seen in nine instances (5.1 %). Four of these areas were associated with central papillary atrophy; the remaining five were covered by non‐atrophic mucosa with filiform and fungiform papillae.
The inflammatory infiltrate in lichen planus lesions
Authors: Walker D. M. 
Published: April 2006

Abstract.The labeling index of the infiltrate of oral lichen planus lesions was significantly greater than that of normal mucosa, and its labeling activity redistributed, as determined by in vitro autoradiography. An electron microscopic examination of the infiltrate of lichen planus lesions of oral mucosa and skin supported the concept that the lichen planus infiltrate represents a cell mediated immune response in which T lymphocytes and macrophages are predominant.
Granular cell peripheral odontogenic fibroma
Authors: Lownie J. F. , Altini M. , Shear M. 
Published: April 2006

Abstract.A case of a peripheral odontogenic fibroma which contained aggregates of large granular cells is reported. These granular cells are similar to those previously described in the granular cell myoblastoma, congenital epulis and the granular cell ameloblastic fibroma.Deep extensions of the basal layer of overlying gingival epithelium, in double‐strand fashion, are frequently observed in peripheral odontogenic fibromas. These strands closely resemble those seen in the tumor itself. On this basis, and as similar basal cell prolongations are seen in other jaw lesions, it is postulated that residual ectomesenchymal influence may be responsible for inducing the basal cell proliferations in a similar manner to that which occurs during early embryonic dental development. This, it is suggested, might possibly be the histogenesis of the odontogenic epithelial strands in the peripheral odontogenic fibroma.
Pulpal response to a new visible light‐cured composite restorative material: Fotofil®
Authors: Bloch W. W. , Austin J. C. , Cleaton‐Jones P. E. , Wilton‐Cox H. , Fatti L. P. 
Published: April 2006

Abstract A new visible light‐cured composite resin, Fotofil®, has been developed for the esthetic restoration of anterior teeth. The pulpal responses elicited by this material in unlined cavities and cavities lined with Dycal® were compared with those elicited by a negative control consisting of modified zinc oxide‐eugenol cement Nobetec® and a silicate cement Syntrex®. The pulpal responses were evaluated in the permanent maxillary and mandibular incisors in vervet monkeys using labial Class V cavities prepared in a total of 240 teeth. Two teeth were filled with each material in each monkey. Groups of 10 monkeys were killed at intervals of 2 days, and 2 and 8 weeks after restoration of the teeth.The most severe pulpal responses were elicited by the unlined Fotofil® and Syntrex® restorations at all three time intervals. The Dycal® lined Fotofil® restorations elicited a milder pulp response than the Nobetec ® at 2 days and 2 weeks. No pulpal response was elicited by the latter two materials at 8 weeks.These results clearly showed that Fotofil® and Syntrex® are irritant to the dental pulp up to 8 weeks. The placement of a Dycal® liner beneath Fotofil® restorations reduced the pulpal irritation elicited by this material to levels which were lower than those elicited by the Nobetec® control at 2 days and 2 weeks.
Microprobe and microradiographic studies of hyalin bodies in odontogenic cysts
Authors: Allison R. T. 
Published: April 2006

Abstract.Microprobe analysis of Araldite embedded sections of hyalin bodies revealed the presence of sulphur, chlorine, calcium and in some cases iron. The results have been compared with those obtained from control tissue sections of human heel skin, a thrombus occluded vessel and mineralized spherules in a dental follicle. Microradiographic studies showed that the density of hyalin bodies increased progressively towards the core. Isodensitracing confirms the laminar configuration of hyalin bodies as seen electron microscopically. The findings conform with the hypothesis that hyalin bodies originate as an epithelial secretion.
A fine structural comparison of the healing of incisional wounds of mucosa and skin
Authors: Sciubba James J. , Waterhouse John P. , Meyer Julia 
Published: April 2006

Abstract Incisional wounds of the same length and depth were made on tongue and dorsal skin of rats and the fine structural aspects of the responses of these were compared. Comparisons were made between the timing and degree of phagocytosis, the timing and rate of epithelial cell migration, of overall rate of healing, and of reformation of basal lamina and attachment complex in these two tissues. Phagocytic activity in epithelium and connective tissue of mucosa reached higher levels than in skin and the peak of activity in the epithelium of the mucosa was earlier than in the epidermis. Mononuclear phagocytes were active in phagocytosis, neutrophils showed little phagocytic activity‐Epithelial migration was found to begin earlier in mucosa than in skin. Epithelialization of the wound and repair of supporting tissue were completed earlier. The same sequence in the renewal of ultrastructural components of the hemidesmosome‐attachment complex was found in skin and mucosa, but was completed earlier at the mucosal site.Mechanical and physical as well as physiologic factors may play a role in determining the differing rates of repair in mucosa and skin.
Light and ultrastructural relationship between oxytalan fibers in the periodontal ligament of the guinea pig
Authors: Edmunds R. S. , Simmons T. A. , Cox C. F. , Avery J. K. 
Published: April 2006

Abstract The interfaces and the relationships between collagen and oxytalan fibers were observed d light and electron microscopy. Guinea pig periodontal ligament was prepared for light and electron microscopy with perfusion using Peter's buffered formalin for light microscopy and GTA‐S‐collidine and OSO4 for electron microscopic studies. The tissue for light microscopy was stained with a modified Gomori's aldehyde fuchsin technique, in which pre‐oxidization with potassium monopersulfate was carried out before staining so as to demonstrate the oxytalan fibers. EM tissues were routinely stained with lead citrate and uranyl acetate.Two different structural relationships were observed. First, the subcomponents of the collagen and oxytalan fiber types interweave with each other; and, second, some of these two‐fiber subcomponents appear attached to each other. These relationships and the known orientation of oxytalan fibers as seen in the periodontal ligament provide insight as to the function of oxytalan fibers. The oxytalan fibers may provide increased structural integrity and increased distribution of forces over a wider area of the periodontal ligament. Because of their close relationship to blood and lymph vessels in the periodontal ligament, they may also help to stabilize these elements by the same structural relationships to collagen fibers.
A one‐year follow‐up of partial pulpotomy and calcium hydroxide capping in primary molars
Authors: Schröder Ulla , Szpringer‐Nodzak Maria , Janicha Jadwiga , Wacińska Maria , Budny Jerzy , Mlosek Krzysztof 
Published: April 2006

Abstract Partial pulpotomy and capping with calcium hydroxide was performed in 93 lower primary molars. The pulpal diagnosis was chronic coronal pulpitis. The amputation was done with a gentle technique, using high speed and diamond burs. Calcium hydroxide was applied to the wound surface without any intermediate layer of blood clot. The cavity was sealed with slow‐setting zinc oxide‐eugenol cement, phosphate cement and silver amalgam.The teeth were followed clinically and roentgenologically. After one year 67 successful endodontic treatments and 14 failures were registered. The remaining 12 teeth were lost for reasons unconnected with the endodontic treatment. The frequency of successful treatments, estimated with the aid of a modified life table method, was 83%.Partial pulpotomy thus proved favorable compared to traditional coronal pulpotomy and should therefore be considered the treatment of choice in primary molars with chronic coronal pulpitis provided a gentle surgical technique is used and no blood clot is left between wound surface and calcium hydroxide.