bluuebunny/crossref_metadata_2025_split huggingface dataset:
Permeability Improvement of Electropolymerized Polypyrrole Films in Water Using Magnetic Hydrophilic Microbeads
Authors: Cosnier Serge , Ding Shou‐Nian , Pellissier Aymeric , Gorgy Karine , Holzinger Michael , Pérez López Briza , Merkoçi Arben 
Published: February 2009

AbstractThe electrochemical polymerization of polypyrrole films onto magnetically immobilized hydrophilic microbeads was carried out. Poly(pyrrole‐pyrene)‐microbeads coatings display thus higher permeability in water towards the diffusion of ferrocyanide than a pure poly(pyrrole‐pyrene) film. In addition, the modulation of this permeability through an applied magnetic field that attracts the microbeads on the electrode surface or pushes back them was shown. Moreover, a glucose biosensor was prepared by adsorbing and electropolymerizing on a platinum electrode, an aqueous mixture containing glucose oxidase, amphiphilic pyrrole monomer and microbeads attracted by a magnet. The presence of microbeads inside the biocoating, markedly increases the biosensor performance.
Ordered Mesoporous Carbon Functionalized with Polythionine for Electrocatalytic Application
Authors: Qi Bin , Peng Xiaojuan , Fang Jue , Guo Liping 
Published: February 2009

AbstractA polythionine (PTH) functionalized ordered mesoporous carbon (OMC) material (PTH/OMC) was presented. The electrochemistry kinetic characteristics of this material are investigated and compared with pure OMC. The results showed that compared with OMC, PTH/OMC possesses a much higher electron transfer rate. For the application of this material, an electrocatalytic based NADH biosensor was constructed on glassy carbon electrode (GCE). Instead of 0.592 V on bare GCE and 0.206 V on OMC/GCE, the amperometric detection of NADH could be effectively performed on the present biosensor with operation potential be set at 0.0 V. In addition, the sensor showed good reproducibility and stability.
Selective Detection of Dopamine Using Glassy Carbon Electrode Modified by a Combined Electropolymerized Permselective Film of Polytyramine and Polypyrrole‐1‐propionic Acid
Authors: Zhou Lin , Shang Fengjun , Pravda Mila , Glennon Jeremy D. , Luong John H. T. 
Published: February 2009

AbstractA sensitive and selective electrochemical method for the determination of dopamine using a combined electropolymerized permselective film of polytyramine and polypyrrole‐1‐propionic acid on a glassy carbon (GC) electrode was developed. The formation of a “layer‐by‐layer” film has allowed for selective detection of dopamine in the presence of 3,4‐dihydroxyphenylalanine (L‐DOPA), DOPAC, ascorbic acid, uric acid, epinephrine and norepinephrine. The modified electrodes exhibited a detection limit of 100 nM with linearity ranging from 5×10−6 to 5×10−5 M. No cleaning step was required during the course of repeated measurement.
Joule heating effects and the experimental determination of temperature during CE
Authors: Evenhuis Christopher J. , Haddad Paul R. 
Published: February 2009

AbstractJoule heating is ubiquitous in electrokinetic separations. This review is in two major parts. The first part documents the effects of Joule heating on the physical properties of the electrolyte and efficiency of separations and the second part focuses on advances in the determination of electrolyte temperatures that have been described in the literature over the past 5 years. The focus is on methods that can be applied by practitioners without the need for elaborate experimental requirements. Although the emphasis is on CE, many of the conclusions also apply to microfluidic formats.
Modulation of histone deacetylase activity by dietary isothiocyanates and allyl sulfides: Studies with sulforaphane and garlic organosulfur compounds
Authors: Nian Hui , Delage Barbara , Ho Emily , Dashwood Roderick H. 
Published: February 2009

AbstractHistone deacetylase (HDAC) inhibitors reactivate epigenetically‐silenced genes in cancer cells, triggering cell cycle arrest and apoptosis. Recent evidence suggests that dietary constituents can act as HDAC inhibitors, such as the isothiocyanates found in cruciferous vegetables and the allyl compounds present in garlic. Broccoli sprouts are a rich source of sulforaphane (SFN), an isothiocyanate that is metabolized via the mercapturic acid pathway and inhibits HDAC activity in human colon, prostate, and breast cancer cells. In mouse preclinical models, SFN inhibited HDAC activity and induced histone hyperacetylation coincident with tumor suppression. Inhibition of HDAC activity also was observed in circulating peripheral blood mononuclear cells obtained from people who consumed a single serving of broccoli sprouts. Garlic organosulfur compounds can be metabolized to allyl mercaptan (AM), a competitive HDAC inhibitor that induced rapid and sustained histone hyperacetylation in human colon cancer cells. Inhibition of HDAC activity by AM was associated with increased histone acetylation and Sp3 transcription factor binding to the promoter region of the P21WAF1 gene, resulting in elevated p21 protein expression and cell cycle arrest. Collectively, the results from these studies, and others reviewed herein, provide new insights into the relationships between reversible histone modifications, diet, and cancer chemoprevention. Environ. Mol. Mutagen., 2009. © 2009 Wiley‐Liss, Inc.
Vascular endothelial growth factor gene polymorphisms are associated with the risk of developing adenomyosis
Authors: Kang Shan , Zhao Jian , Liu Qing , Zhou Rongmiao , Wang Na , Li Yan 
Published: February 2009

AbstractVascular endothelial growth factor (VEGF), a major mediator of angiogenesis and vascular permeability, may play a key role in the development of adenomyosis. The aim of this study was to investigate whether these four VEGF polymorphisms (−2578C/A, −1154G/A, −460C/T, and +936C/T) were associated with the risk of adenomyosis development. Genotypes were determined by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) assay in 174 adenomyosis patients and 199 frequency‐matched control women. There were significant differences between patients and control group in allele frequencies and genotype distributions of the −2578C/A polymorphisms (P = 0.010 and 0.044, respectively). Compared with the C/C genotype, the A/A + C/A genotype could significantly modify the risk of developing adenomyosis [odds ratio (OR) = 0.64, 95% confidence interval (CI) = 0.42–0.97]. For the −1154G/A polymorphism, the allele frequencies and genotype distributions in patient group were significant different from those of the controls (P = 0.001 and 0.007, respectively). Compared with the G/G genotype, the A/A + G/A genotype could significantly decrease the risk of developing adenomyosis (OR = 0.51, 95% CI = 0.33–0.80). However, the genotype distributions and allele frequencies of the −460C/T and +936C/T polymorphisms did not significantly differ between controls and patients (all P value > 0.05). The haplotype analysis suggested that the TGA (VEGF −460/−1154/−2578) and CGA haplotypes exhibited a significant decrease in the risk of developing adenomyosis compared with the haplotype of TGC (OR = 0.64, 95% CI = 0.41–1.00; OR = 0.44, 95% CI = 0.21–0.93, respectively). The study indicated that the −2578A or −1154A allele of VEGF gene could significantly decrease the risk of adenomyosis and might be potentially protective factors for adenomyosis development. Environ. Mol. Mutagen., 2009. © 2009 Wiley‐Liss, Inc.
Concentrated flow erosion rates reduced through biological geotextiles
Authors: Smets T. , Poesen J. , Langhans C. , Knapen A. , Fullen M. A. 
Published: February 2009

AbstractSoil erosion by concentrated flow can cause serious environmental damage. Erosion‐control geotextiles have considerable potential for reducing concentrated flow erosion. However, limited data are available on the erosion‐reducing potential of geotextiles. In this study, the effectiveness of three biological geotextiles in reducing soil losses during concentrated flow is investigated. Hereto, runoff was simulated in a concentrated flow flume, filled with an erodible sandy loam on three slope gradients (13·5, 27·0 and 41·5%). Treatments included three biological geotextiles (borassus, buriti and bamboo) and one bare soil surface. Darcy–Weisbach friction coefficients ranged from 0·01 to 2·84. The highest values are observed for borassus covered soil surfaces, followed by buriti, bamboo and bare soil, respectively. The friction coefficients are linearly correlated with geotextile thickness. For the specific experimental conditions of this study, borassus geotextiles reduced soil detachment rate on average to 56%, buriti geotextiles to 59% and bamboo geotextiles to 66% of the soil detachment rate for bare soil surfaces. Total flow shear stress was the hydraulic parameter best predicting soil detachment rate for bare and geotextile covered surfaces (R2 = 0·75–0·84, p <0·001, n = 12–15). The highest resistance against soil detachment was observed for the borassus covered soil surfaces, followed by buriti, bamboo and bare soil surfaces, respectively. Overall, biological geotextiles are less effective in controlling concentrated flow erosion compared with interrill erosion. Copyright © 2009 John Wiley & Sons, Ltd.
Morphodynamics of a 1000‐year flood in the Kamp River, Austria, and impacts on floodplain morphology
Authors: Hauer C. , Habersack H. 
Published: February 2009

AbstractThis paper investigates the impact of a 1000‐year flood in August 2002 on floodplains and valley morphology of an Austrian mixed alluvial bed rock river. Discharges with a recurrence interval between 500 and 2000 years caused distinctive overbank scouring and material deposition in the floodplains. After the 1000‐year flood, those morphologically affected areas were at random intervals documented over the whole longitudinal profile. In addition to overbank erosion in curved sections (cut‐offs), the river bed locally widened, floodplain stripping occurred and local overbank scours were documented along straight parts of the river. A hydrodynamic‐numerical model, combined with field measurements, was used to analyse the cause of these erosional landforms. Based on the modelled hydraulic conditions for a one‐year flood (30–78 m3 s–1) and the catastrophic 2002 event (700–800 m3 s–1), the numerical results allowed a cause‐effect study with 19 parameters. Deterministic and statistical analysis (ANOVA, discriminant analysis) showed that the morphodynamic effects of the 2002 flood were influenced by the variability of valley morphology of the Kamp River, which led partially to supercritical flow during flood constriction. These processes were in some cases also anthropogenically influenced. Lateral constriction and expansion of the valley geometry over short distances led to scouring and aggradation within the inundated areas during the event. These morphological features were therefore responsible for the elongated scour holes in the floodplains. Copyright © 2009 John Wiley & Sons, Ltd.
Scale‐dependent controls upon the fluvial export of large wood from river catchments
Authors: Seo Jung Il , Nakamura Futoshi 
Published: February 2009

AbstractThe annual fluvial export of large wood (LW) was monitored by local reservoir management offices in Japan. LW export per unit watershed area was relatively high in small watersheds, peaked in intermediate watersheds, and decreased in large watersheds. To explain these variations, we surveyed the amount of LW with respect to channel morphology in 78 segments (26 segments in each size class) in the Nukabira River, northern Japan. We examined the differences in LW dynamics, including its recruitment, transport, storage, and fragmentation and decay along the spectrum of watershed sizes. We found that a large proportion of LW produced by forest dynamics and hillslope processes was retained because of the narrower valley floors and lower stream power in small watersheds. The retained LW pieces may eventually be exported during debris flows. In intermediate watersheds, the volume of LW derived from hillslopes decreased substantially with reductions in the proportion of channel length bordered by hillslope margins, which potentially deliver large quantities of LW. Because these channels have lower wood piece length to channel width ratios and higher stream power, LW pieces can be transported downstream. During transport, LW pieces are further fragmented and can be more easily transported. Therefore, the fluvial export of LW is maximized in intermediate watersheds. Rivers in large watersheds, where the recruitment of LW is limited by the decreasing hillslope margins, cannot transport LW pieces because of their low stream power, and thus LW pieces accumulate at various storage sites. Although these stored LW pieces can be refloated and transported by subsequent flood events, they may also become trapped by obstacles such as logjams and standing trees on floodplains and in secondary channels, remaining there for decades and eventually decaying into fine organic particles. Thus, the fluvial export of LW pieces is low in large watersheds. Copyright © 2009 John Wiley & Sons, Ltd.
Geoarchaeological investigation of lithic resources in the central Mesa Verde region, Colorado, USA
Authors: Arakawa Fumiyasu , Miskell‐Gerhardt Kimberlee 
Published: February 2009

AbstractAn important question in Southwestern archaeological research is how social and economic ties between prehistoric groups changed through time. The chemical and mineralogical matching of stone tools and ceramics to source areas is one approach to addressing this question. Here we challenge the assumption that igneous rocks used for manufacturing stone tools and ceramics in the Mesa Verde region of Colorado were originally derived from the Chuska Mountains of New Mexico. Recent geological surveys suggest that small igneous features present in and around Mesa Verde National Park and Ute Mountain Ute Tribal Park contain similar lithologies and may represent a local source. We systematically visited seven of these igneous exposures and nine downstream gravel deposits, looking for geological sources and quarry sites matching the lithology of analyzed debitage from this region. Our results suggest that the ancestral Puebloans could have procured such igneous rocks as aphanitic minette and trachybasalt pottery temper from local areas. Because the trachybasalt is usually thought to have been imported from the Chuska Mountains, this discovery may require a revision of interpreted trade networks based on pottery temper and lithic raw material distinctions. © 2009 Wiley Periodicals, Inc.
VALID: visualization of association study results and linkage disequilibrium
Authors: Jorgenson Eric , Kvale Mark , Witte John S. 
Published: February 2009

AbstractPromising findings from genetic association studies are commonly presented with two distinct figures: one gives the association study results and the other indicates linkage disequilibrium (LD) between genetic markers in the region(s) of interest. Fully interpreting the results of such studies requires synthesizing the information in these figures, which is generally done in a subjective and unsystematic manner. Here we present a method to formally combine association results and LD and display them in the same figure; we have developed a freely available web‐based application that can be used to generate figures to display the combined data. To demonstrate this approach we apply it to fine mapping data from the prostate cancer 8q24 loci. Combining these two sources of information in a single figure allows one to more clearly assess patterns of association, facilitating the interpretation of genome‐wide and fine mapping data and improving our ability to localize causal variants. Genet. Epidemiol. 33:599–603, 2009. © 2009 Wiley‐Liss, Inc.
Somatic alterations, metabolizing genes and smoking in rectal cancer
Authors: Curtin Karen , Samowitz Wade S. , Wolff Roger K. , Herrick Jennifer , Caan Bette J. , Slattery Martha L. 
Published: February 2009

AbstractCigarette smoking has been identified as a risk factor for rectal cancer. Our investigation evaluates associations between active and passive smoking and TP53, KRAS2, and BRAF V600E mutations, microsatellite instability (MSI), and CpG Island Methylator Phenotype (CIMP) in rectal tumors. We examine how genetic variants of GSTM1 and NAT2 alter these associations in a population‐based, case–control study of 750 incident rectal cancer cases and 1,201 controls. Detailed tobacco exposure data were collected in an extensive questionnaire. DNA from blood was examined for GSTM1 and NAT2 variants. Tumor DNA was assessed to determine TP53 (exons 5–8), KRAS2 (codons 12–13) and BRAF mutations, MSI (BAT26 and TGFβRII analysis), and CIMP (methylation of CpG islands in CDKN2A, MLH1, MINT1, MINT2 and MINT31). Cigarette smoking (>20 pack‐years, relative to nonsmokers) was associated with increased risk of TP53 mutations (OR = 1.4, 95% CI 1.02–2.0), BRAF mutations (OR = 4.2, 95% CI 1.3–14.2) and MSI (OR = 5.7, 95% CI 1.1–29.8) in rectal tumors. Long‐term environmental tobacco smoke (ETS) exposure of >10 hr/wk was associated with increased risk of KRAS2 mutation (OR = 1.5, 95% CI 1.04–2.2). All smoking indicators were suggestive of increased risk in CIMP+ rectal cancer. GSTM1 and NAT2 were generally not associated with rectal tumor alterations; however, we observed an interaction of ETS and NAT2 in TP53‐mutated tumors (p < 0.01). Our investigation shows active smoking is associated with increased risk of TP53, BRAF and MSI+ in rectal tumors and is suggestive of increased risk of CIMP+ tumors. ETS may increase risk of KRAS2 mutations; association with TP53 mutations and ETS may be influenced by NAT2. © 2009 UICC
Incidence of human papillomavirus (HPV) positive tonsillar carcinoma in Stockholm, Sweden: An epidemic of viral‐induced carcinoma?
Authors: Näsman Anders , Attner Per , Hammarstedt Lalle , Du Juan , Eriksson Mathilda , Giraud Geraldine , Ährlund‐Richter Sofie , Marklund Linda , Romanitan Mircea , Lindquist David , Ramqvist Torbjörn , Lindholm Johan , Sparén Pär , Ye Weimin , Dahlstrand Hanna , Munck‐Wikland Eva , Dalianis Tina 
Published: February 2009

AbstractIn the county of Stockholm, between 1970 and 2002, we have previously reported a 3‐fold parallel increase in the incidence of tonsillar squamous cell carcinoma (SCC) and the proportion of human papillomavirus (HPV) positive tonsillar SCC. Here, we have followed the above parameters in all patients (n = 120) diagnosed with tonsillar SCC during 2003–2007 in the same area, and also in correlation to our previous data. Ninety‐eight pretreatment biopsies were available and presence of HPV DNA and HPV‐16 E6 and E7 RNA were tested by polymerase chain reaction (PCR) and RT‐PCR. Incidence data were obtained from the Swedish Cancer Registry. Data reported from 1970 to 2002 were also obtained for comparison. HPV DNA was present in 83 of 98 (85%) of the tonsillar SCC biopsies from 2003 to 2007 and 77 of these were HPV‐16 positive. HPV‐16 E6 and E7 RNA were found in 98% of 52 analyzed HPV‐16 positive cases. The proportion of HPV‐positive cancers had significantly increased both from 1970 to 2007 (p < 0.0001) as well from 2000 to 2007 (p < 0.01), with 68% (95% confidence interval (CI), 53–81) 2000–2002; 77% (95% CI, 63–87) 2003–2005; and 93% (95% CI, 82–99) 2006–2007. The incidence rate of HPV‐positive tumors almost doubled each decade between 1970 and 2007, in parallel with a decline of HPV‐negative tumors. In conclusion, the incidence of HPV‐positive cancers is still increasing in the County of Stockholm, suggesting an epidemic of a virus‐induced carcinoma, with soon practically all tonsillar SCC being HPV positive, as in cervical cancer. © 2009 UICC
Downregulation of the c‐MYC target gene, peroxiredoxin III, contributes to arsenic trioxide‐induced apoptosis in acute promyelocytic leukemia
Authors: Vivas‐Mejía Pablo E. , Ozpolat Bulent , Chen Xian , Lopez‐Berestein Gabriel 
Published: February 2009

AbstractArsenic trioxide (ATO) induces differentiation and apoptosis in acute promyelocytic leukemia (APL). Several reports indicate that in APL cells apoptosis occurs mainly by a mechanism that involves the inhibition of glutathione peroxidase, one of the enzymes that regulates mitochondrial levels of H2O2. Peroxiredoxin (Prx) III, a c‐MYC target gene, is also a mitochondria‐specific H2O2‐scavenger enzyme. We studied here the role of Prx III during ATO‐induced apoptosis in APL‐derived NB4 cells, since these cells express high levels of Prx III. The protein and mRNA levels of Prx III decreased during ATO‐induced apoptosis of NB4 cells. The downregulation of Prx III occurred before reactive oxygen species accumulation, reduction in the mitochondrial membrane potential and apoptosis. Depletion of Prx III enhanced mitochondrial‐dependent apoptosis events. In contrast, overexpression of Prx III led to reduced levels of ATO‐induced apoptosis. c‐MYC was also downregulated in ATO‐treated NB4 cells. Furthermore, depletion of c‐MYC also reduced the Prx‐III expression levels. Finally chromatin immunoprecipitation and luciferase reporter assays confirmed that downregulation of Prx‐III was caused by the reduction of c‐MYC levels during ATO‐induced apoptosis of NB4 cells. These findings demonstrate a novel apoptotic‐response pathway whereby downregulation of Prx‐III potentiates ATO‐induced apoptosis in APL cells. © 2009 UICC
Downregulation of connective tissue growth factor by three‐dimensional matrix enhances ovarian carcinoma cell invasion
Authors: Barbolina Maria V. , Adley Brian P. , Kelly David L. , Shepard Jaclyn , Fought Angela J. , Scholtens Denise , Penzes Peter , Shea Lonnie D. , Stack M. Sharon 
Published: February 2009

AbstractEpithelial ovarian carcinoma (EOC) is a leading cause of death from gynecologic malignancies, due mainly to the prevalence of undetected metastatic disease. The process of cell invasion during intraperitoneal anchoring of metastatic lesions requires concerted regulation of many processes, including modulation of adhesion to the extracellular matrix and localized invasion. Exploratory cDNA microarray analysis of early response genes (altered after 4 hr of 3D collagen culture) coupled with confirmatory real‐time reverse‐transcriptase polymerase chain reaction, multiple 3D cell culture matrices, Western blot, immunostaining, adhesion, migration and invasion assays were used to identify modulators of adhesion pertinent to EOC progression and metastasis. cDNA microarray analysis indicated a dramatic downregulation of connective tissue growth factor (CTGF) in EOC cells placed in invasion‐ mimicking conditions (3D Type I collagen). Examination of human EOC specimens revealed that CTGF expression was absent in 46% of the tested samples (n = 41), but was present in 100% of normal ovarian epithelium samples (n = 7). Reduced CTGF expression occurs in many types of cells and may be a general phenomenon displayed by cells encountering a 3D environment. CTGF levels were inversely correlated with invasion such that downregulation of CTGF increased, while its upregulation reduced collagen invasion. Cells adhered preferentially to a surface comprised of both collagen I and CTGF relative to either component alone using α6β1 and α3β1 integrins. Together these data suggest that downregulation of CTGF in EOC cells may be important for cell invasion through modulation of cell‐matrix adhesion. © 2009 UICC