bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kernicterus by glucose-6-phosphate dehydrogenase deficiency: a case report and review of the literature
Authors: Cossio de Gurrola Gladys , Araúz Juan José , Durán Elfilda , Aguilar-Medina Maribel , Ramos-Payán Rosalío , García-Magallanes Noemí , Pacheco Gerardo Vaca , Arámbula Meraz Eliakym 
Published: May 2008

Abstract

Introduction
Glucose-6-phosphate dehydrogenase deficiency is an X-linked recessive disease that causes acute or chronic hemolytic anemia and potentially leads to severe jaundice in response to oxidative agents. This deficiency is the most common human innate error of metabolism, affecting more than 400 million people worldwide.

Case presentation
Here, we present the first documented case of kernicterus in Panama, in a glucose-6-phosphate dehydrogenase-deficient newborn clothed in naphthalene-impregnated garments, resulting in reduced psychomotor development, neurosensory hypoacousia, absence of speech and poor reflex of the pupil to light.

Conclusion
Mutational analysis revealed the glucose-6-phosphate dehydrogenase Mediterranean polymorphic variant, which explained the development of kernicterus after exposition of naphthalene. As the use of naphthalene in stored clothes is a common practice, glucose-6-phosphate dehydrogenase testing in neonatal screening could prevent severe clinical consequences.
The genome sequence of the model ascomycete fungus Podospora anserina
Authors: Espagne Eric , Lespinet Olivier , Malagnac Fabienne , Da Silva Corinne , Jaillon Olivier , Porcel Betina M , Couloux Arnaud , Aury Jean-Marc , Ségurens Béatrice , Poulain Julie , Anthouard Véronique , Grossetete Sandrine , Khalili Hamid , Coppin Evelyne , Déquard-Chablat Michelle , Picard Marguerite , Contamine Véronique , Arnaise Sylvie , Bourdais Anne , Berteaux-Lecellier Véronique , Gautheret Daniel , de Vries Ronald P , Battaglia Evy , Coutinho Pedro M , Danchin Etienne GJ , Henrissat Bernard , Khoury Riyad EL , Sainsard-Chanet Annie , Boivin Antoine , Pinan-Lucarré Bérangère , Sellem Carole H , Debuchy Robert , Wincker Patrick , Weissenbach Jean , Silar Philippe 
Published: May 2008

Abstract
Background
The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.

Results
We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown.

Conclusion
The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.
Finite Element Modeling of the Effects of Delamination Damage on the Natural Frequencies of Composite Panels
Authors: Deshmukh P. V. , Wosu S. N. , Daniel L. 
Published: May 2008

Abstract
This paper describes the analysis and experimental effort undertaken to determine damage detection in composite beams. Finite element analysis of delamination and its effect on the natural frequencies of the composite beam is determined using cohesive zone elements of a commercial finite element software material model ANSYS. Numerical simulations are carried out to analyze typical cases of a delaminated composite beam with different boundary conditions. It is found that an increase in the delaminated region ratio increases the percent deviation in the natural system frequencies. The amount of deviation was different for different types of delamination. The frequency charts of the present study may be useful for engineering design and non-destructive testing analysis. It can be used as a tool for diagnosis, detection, and identification of delaminations in engineering applications by monitoring the frequency change.
The Human Lipodystrophy Gene BSCL2/Seipin May Be Essential for Normal Adipocyte Differentiation
Authors: Payne Victoria A. , Grimsey Neil , Tuthill Antoinette , Virtue Sam , Gray Sarah L. , Dalla Nora Edoardo , Semple Robert K. , O'Rahilly Stephen , Rochford Justin J. 
Published: May 2008

OBJECTIVE—Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) is a recessive disorder featuring near complete absence of adipose tissue. Remarkably, although the causative gene, BSCL2, has been known for several years, its molecular function and its role in adipose tissue development have not been elucidated. Therefore, we examined whether BSCL2 is involved in the regulation of adipocyte differentiation and the mechanism whereby pathogenic mutations in BSCL2 cause lipodystrophy.
RESEARCH DESIGN AND METHODS—Following the characterization of BSCL2 expression in developing adipocytes, C3H10T1/2 mesenchymal stem cells were generated in which BSCL2 expression was knocked down using short hairpin RNA (shRNA). These cells were used to investigate whether BSCL2 is required for adipogenesis. BSCL2 constructs harboring pathogenic mutations known to cause lipodystrophy were also generated and characterized.
RESULTS— BSCL2 expression was strongly induced during adipocyte differentiation, and the induction of BSCL2 expression was essential for adipogenesis to occur. The initial induction of key adipogenic transcription factors, including peroxisome proliferator–activated receptor (PPAR)γ and CAAT/enhancer-binding protein-α, was preserved in cells lacking BSCL2. However, the expression of these critical factors was not sustained, suggesting that the activity of PPARγ was impaired. Moreover, expression of key genes mediating triglyceride synthesis, including AGPAT2, lipin 1, and DGAT2, was persistently reduced and lipid accumulation was inhibited. Analysis of pathogenic missense mutants of BSCL2 revealed that the amino acid substitution A212P causes aberrant targeting of BSCL2 within the cell, suggesting that subcellular localization of BSCL2 may be critical to its function.
CONCLUSIONS—This study demonstrates that BSCL2 is an essential, cell-autonomous regulator of adipogenesis.
Effects of age, period, and cohort on stroke mortality among the Lithuanian urban population during the 24-year follow-up period
Authors: Rėklaitienė Regina , Noreika Marius , Tamošiūnas Abdonas , Virvičiūtė Dalia , Šopagienė Diana 
Published: May 2008

Abstract
The main purpose of this paper was to assess the effects of age, period, and cohort on stroke mortality among the urban Lithuanian population. Routine stroke mortality data among the Lithuanian urban population aged 25–64 years (1041 men and 724 women) between 1980 and 2004 were obtained from the official Kaunas region mortality register and classified by codes 430–438 and 160–169 in the 9th and 10th revisions of the International Classifications of Diseases (ICD), respectively. Mortality rates per 100,000 persons for men and women were age-adjusted using the age distribution of the European Standard Population. Goodness of fit of the Poisson regression models was evaluated using the Pearson and Freeman-Tukey residuals. During the study period, mortality rates decreased from 46.8 to 33.0 per 100,000 for men, and from 20.2 to 18.1 per 100,000 for women (average annual decrease of −1.3%, p<0.1 for men, and −1.6%, p<0.03 for women). An age effect was present in both sexes. The definite upward period effect was observed from 1990 to 1994 both among men and women, and was followed by a sharp fall during 2000–2004. Cohort and period effects have contained relevant information that partially explained trends in stroke mortality among 25–64 year-olds in the Lithuanian urban population. The Poisson regression models could be applied for the examination and explanation of the different causes of the population mortality.
Thermal, mechanical, and dielectric properties of novel fluorinated copoly(imide siloxane)s
Authors: Ghosh Anindita , Banerjee Susanta 
Published: May 2008

AbstractEight new poly(imidesiloxane)s copolymers have been prepared by one‐pot solution imidization method. The polymers are made by the reaction of bisphenol‐A dianhydride (BPADA) with four different fluorinated diamines and amino‐propyl terminated polydimethylsiloxane (APPS). The polymers are named as 1a, 1b, 1c, and 1d, respectively, with a siloxane loading of 40 wt %. The second series of polymers where siloxane loading is 20 wt % were named as 1e, 1f, 1g, and 1h. The resulting polymers have been well‐characterized by GPC, IR, and NMR techniques. The proton NMR indicates the siloxane loading is about 36–38% for the first series of polymers 1a–1d and a loading of 17–18% for the series of polymers 1e–1h. The films of these polymers showed low water absorption of 0.02% for the first series of polymers and 0.11% for the second series of polymers. Thermal, mechanical, and dielectric properties of these polymers have been evaluated and compared with their nonsiloxane analogues. Preliminary investigations of melt processability of these polymers were also investigated. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Effects of molding pressure on the warpage and the viscoelasticities of HVQFN packages
Authors: Gui D. Y. , Ernst L. J. , Jansen K. M. B. , Yang D. G. , Goumans L. , Bressers H. J. L. , Janssen J. H. J. 
Published: May 2008

AbstractMolding temperature, molding pressure, and time are three major parameters affecting the curing quality of molding materials. In this article, the effect of molding pressure on warpage of HVQFN packages is studied. The typical map‐chips with 65% silica particle‐filled epoxy resin are manufactured under five molding pressure levels from 1.8 to 12.3 MPa. The curvature measurements are performed for the packages after demolding and postcuring, respectively. The viscoelastic tensile relaxation modulus of molding materials is obtained by using dynamic mechanical analysis. The characterization of evolution of equilibrium moduli is analyzed for future modification of the model. The experimental results show that the molding pressure has a significant effect on the warpage after molding as well as after postcuring of HVQFN packages. The curvatures of HVQFN packages at both lower (1.8 MPa) and higher molding pressures (12.27 MPa) are about 45% less than the average max curvature at 3.6–8.66 MPa. The molding pressure has obvious influences on the glassy Young's modulus but has little effects on the rubbery modulus and relaxation time of the package materials. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Crosslinking of mixtures of DGEBA with 1,6‐dioxaspiro[4,4]nonan‐2,7‐dione initiated by tertiary amines. I. Study of the reaction and kinetic analysis
Authors: Fernández‐Francos Xavier , Salla Josep M. , Mantecón Ana , Serra Àngels , Ramis Xavier 
Published: May 2008

AbstractThe anionic copolymerization of a Diglycidyl ether of bisphenol A (DGEBA) epoxy resin and a bislactone has been studied with different initiators. The kinetics of the process has been dealt with in detail, and it has been detected the existence of different competing curing mechanisms: a quasi‐alternating copolymerization between the epoxy monomer and the bislactone, and homopolymerization of the epoxy resin. In presence of an excess of DGEBA, the copolymerization first takes place, and then the excess of epoxy monomer can homopolymerize. The bislactone induces an apparent accelerating effect because it reduces the extent of epoxy homopolymerization and therefore the termination reactions associated with it, thus allowing a complete cure with a reduced amount of initiator. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Are individuals with lower neutrophil oxidative burst activity more prone to Helicobacter pylori infection?
Authors: Matsuzaka Masashi , Fukuda Shinsaku , Yamai Kiyonori , Tsuya Ryousuke , Fukuoka Yu , Takahashi Ippei , Yaegaki Makoto , Shimoyama Tadashi , Sakamoto Juichi , Umeda Takashi , Nakaji Shigeyuki 
Published: May 2008

AbstractHelicobacter pylori infection has been reported to cause enhanced reactive oxygen species in the gastric mucosa. We examined the relationship between H. pylori infection and neutrophil function of peripheral blood. The subjects were 904 volunteers who participated in the Iwaki Health Promotion Project in 2005. 158 subjects who were infected with H. pylori in 2005 also participated in this project in 2006 and were categorized into two groups: the eradication group, in which H. pylori was successfully eradicated during the 12 month period, and the non‐eradication group, in which eradication was unsuccessful or the subjects did not receive eradication therapy. The laboratory assays performed were: a titre of H. pylori antibody; neutrophil counts; and oxidative burst activity (OBA) of neutrophils. Logistic regression analysis was executed, with H. pylori infection as the dependent variable and other items as the independent variables. OBA showed an inverse association with H. pylori infection in 2005. Additionally, when comparing the eradication and non‐eradication groups, the change rates of OBA between 2005 and 2006 did not show any significant difference. It was concluded that H. pylori infection does not lower OBA, but those individuals in whom OBA was lower were more prone to H. pylori infection. Copyright © 2008 John Wiley & Sons, Ltd.
Flow‐injection determination of cysteine in pharmaceuticals based on luminol–persulphate chemiluminescence detection
Authors: Waseem Amir , Yaqoob Mohammad , Nabi Abdul 
Published: May 2008

AbstractA flow injection (FI) method is reported for the determination of l‐cysteine, based on its enhancement on chemiluminescence (CL) emission of luminol oxidized by sodium persulphate in alkaline solution. The calibration graph was linear over the range 1.0 × 10–9–5.0 × 10–7 mol/L (r2 = 0.9992), with relative standard deviations (RSDs) in the range 1.1–2.3% (n = 4). The limit of detection (3σ blank) was 5.0 × 10–10 mol/L with a sample throughput of 120/h. The method was applied to pharmaceuticals and the results obtained were in reasonable agreement with the amount labelled. The proposed method was also applied to cysteine in synthetic amino acid mixtures. Calibration graphs of N‐acetylcysteine and glutathione over the range 1.0–50 × 10–8 and 0.5–7.5 × 10–7 mol/L were also established (r2 = 0.998 and 0.9986) with RSDs in the range 1.0–2.0% (n = 4), and the limits of detection (3σ blank) were 5.0 × 10–9 and 1.0 × 10–8 mol/L, respectively. Copyright © 2008 John Wiley & Sons, Ltd.
Spectroscopic studies on the interaction of lanthanum(III) 2‐oxo‐propionic acid salicyloyl hydrazone complex with bovine serum albumin
Authors: Zhang Ye‐Zhong , Chen Xiao‐Xia , Dai Jie , Zhang Xiao‐Ping , Liu Yan‐Xia , Liu Yi 
Published: May 2008

AbstractThe interaction of lanthanum(III) 2‐oxo‐propionic acid salicyloyl hydrazone complex (LaIIIL2) with bovine serum albumin (BSA) was studied under physiological conditions. Fluorescence spectroscopy in combination with UV‐vis absorption and circular dichroism (CD) spectroscopy were used to investigate the binding mechanism, binding constants and conformational changes of BSA in the presence of LaIIIL2. It was found that the fluorescence quenching of BSA by LaIIIL2 resulted mainly from the formation of a LaIIIL2–BSA complex. The enthalpy change (δH) and entropy change (δS) were found to be –41.03 kJ/mol and –32.61 J/mol/K, respectively, which indicated that van der Waals' interactions and hydrogen bonds were the predominant intermolecular force in stabilizing the complex. The distance r between the donor (BSA) and acceptor (LaIIIL2) was found to be 4.35 nm, according to Förster theory of non‐radioactive energy transfer. Moreover, the conformational changes of BSA by LaIIIL2 were analysed by means of synchronous fluorescence spectra, CD and three‐dimensional fluorescence spectra. The experiment results confirmed some microenvironmental and conformational changes of BSA molecules in the presence of LaIIIL2. Copyright © 2008 John Wiley & Sons, Ltd.
Measuring neutrophil functions might be a good predictive marker of overtraining in athletes
Authors: Yaegaki Makoto , Umeda Takashi , Takahashi Ippei , Yamamoto Yousuke , Kojima Arata , Tanabe Masaru , Yamai Kiyonori , Matsuzaka Masashi , Sugawara Norio , Nakaji Shigeyuki 
Published: May 2008

AbstractIn order to develop a predictive marker of overtraining in athletes, we examined the changes in neutrophil function [reactive oxygen species (ROS) production capability and phagocytic activity (PA)] for 10 male and 13 female judoists attending a training camp. Measurements were taken four times in total—immediately before and after a 2 h unified exercise loading (UEL) performed 1 day before (Pre‐Camp) and the day after the 7 day training camp (Post‐Camp). UEL‐mediated aspartate aminotransferase was higher at Post‐Camp than at Pre‐Camp in females but not in males. Post‐Camp leukocyte/neutrophil counts after the UEL significantly increased in females but not in males. The rate of change in C4 was significantly smaller in females than in males at Post‐Camp. Only ROS significantly decreased without any compensation (increase in PA) being made at Post‐Camp in females. In conclusion, this finding, namely that ROS significantly decreased only at Post‐Camp without any compensatory mechanism (increase in PA), would suggest that the training camp imposed greater loading on females than males. This consideration was supported by the atypical aspartate aminotransferase, leukocyte/neutrophil counts and C4 findings which were seen at Post‐Camp only in females. Therefore, regularly examining neutrophil functions such as ROS and PA might be a good preventative measure against overtraining in athletes participating in training camps. Copyright © 2008 John Wiley & Sons, Ltd.
A quantitative study of factors affecting in vivo bioluminescence imaging
Authors: Cui Kemi , Xu Xiaoyin , Zhao Hong , Wong Stephen T. C. 
Published: May 2008

AbstractIn vivo bioluminescence imaging (BLI) has the advantages of high sensitivity and low background. By counting the number of photons emitted from a specimen, BLI can quantify biological events such as tumour growth, gene expression and drug response. The intensities and kinetics of the BL signal are affected by many factors and may confound the quantitative results acquired from consecutive imaging sessions or different specimens. We used three different mouse models of tumours to examine whether anaesthetics, positioning and tumour growth may affect the consistency of the BL signal. The results showed that BLI signal could be affected by different anaesthetics and repetitive positioning. Using the same anaesthetics produced consistent peak times, while other factors were held constant. However, as the tumours grew the peak times shifted and the time course of BL signals had different shapes, depending on the positioning of the mice. The data indicate that a carefully designed BLI experiment is required to generate optimal and consistent results. Copyright © 2008 John Wiley & Sons, Ltd.
Oscillating chemiluminescence with thiosemicarbazide in a batch reactor
Authors: Sorouraddin M. H. , Iranifam M. 
Published: May 2008

AbstractOscillating chemical reactions are complex systems involving a large number of chemical species. In oscillating chemical reactions, some species, usually reaction intermediates, exhibit fluctuations in their concentration. In this report, a novel slowly‐damped oscillating chemiluminescence produced by the addition of thiosemicarbazide (TSC) to the oscillating system H2O2–KSCN–CuSO4–NaOH was investigated. Narrow and slightly asymmetric light pulses of 1.5 s half‐width are emitted at 440 nm, with an oscillation period of 22–363 s, an induction period of 9–397 s and an emitted light time of 700–1500 s, depending on reagent concentrations. In this study the dependence of the induction period and the oscillation period on the reagent concentrations was investigated and both parameters were plotted with respect to reagent concentrations. Copper concentration showed a significant effect on the oscillation period. A possible mechanism for the oscillating chemiluminescence reaction is discussed. Copyright © 2008 John Wiley & Sons, Ltd.
Microvalve‐assisted patterning platform for measuring cellular dynamics based on 3D cell culture
Authors: Kim Minseok S. , Lee Wonhye , Kim Yu Chang , Park Je‐Kyun 
Published: May 2008

AbstractA microfluidic platform to satisfy both 3D cell culture and cell‐based assay is required for credible assay results and improved assay concept in drug discovery. In this article, we demonstrate a microvalve‐assisted patterning (MAP) platform to provide a new method for investigating cellular dynamics by generating a linear concentration gradient of a drug as well as to realize 3D cell culture in a microenvironment. The MAP platform was fabricated by multilayer soft lithography and several microvalves made it possible to pattern a cell–matrix (scaffold) and to exchange media solutions without breaking cell–matrix structure in a microchannel. This approach provides not only exact fluids control, bubble removal, and stable solution exchange in a microchannel, but also reliable scaffold fabrication and 3D cell culture. In this study, hepatotoxicity tests with human hepatocellular liver carcinoma cells (HepG2) were also performed in real‐time monitoring where cell morphologies exposed to different drug concentrations were observed at a time. Compared to 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay, the MAP platform could be used to reduce drug amount and assay time for cell‐based assays as much as 10 and 3 times, respectively. Biotechnol. Bioeng. 2008;101: 1005–1013. © 2008 Wiley Periodicals, Inc.
Bullying behaviour in secondary schools: what roles do teachers play?
Authors: James Deborah J. , Lawlor Maria , Courtney Pat , Flynn Ann , Henry Bernie , Murphy Niamh 
Published: May 2008

AbstractTeachers have an important role to play in the management and prevention of bullying. Although many anti‐bullying programmes advocate a ‘whole school approach’, they tend to focus on student‐student behaviours and rarely examine the nature of other relationships which exist in the school. This study examines bullying between students and teachers at two time points. Thirty per cent of students said they were bullied by teachers at both times. Numbers reporting they bullied teachers were 28 per cent and 16 per cent, respectively. The paper explores the important role that teachers play in dealing with bullying in a school setting by modelling appropriate behaviours and dealing with it effectively. If teachers are victimised by or engage in bullying students this has implications for the ethos within the school and may also impact on the success of anti‐bullying programmes. Copyright © 2008 John Wiley & Sons, Ltd.