bluuebunny/crossref_metadata_2025_split huggingface dataset:
A fibrosarcoma model derived from mouse embryo cells: Growth properties and secretion of collagenase and metalloproteinase inhibitor (TIMP) by tumour cell lines
Authors: Hicks Nigel J. , Ward Robin V. , Reynolds John J. 
Published: February 2007

AbstractA new murine fibrosarcoma model has been developed in which it is possible to compare in vitro the behaviour of tumour cells with that of normal parental cells from which the tumour was originally derived by spontaneous transformation in vitro. Tumour cell lines were obtained which showed differing capacities for localized invasion of the skin following subcutaneous injection: these were categorized as either highly invasive or poorly invasive and were compared with the normal cells for (1) their respective saturation densities when grown on plastic, (2) their ability to grow in agar, and (3) their secretions of the metalloenzyme collagenase and the specific inhibitor of metalloproteinases (TIMP). Although increased in vitro saturation density showed some correlation with increased invasiveness in vivo, the most striking correlation was the 10‐ to 20‐fold reduction in TIMP secretion by tumour cells of high invasive potential compared with normal cells or tumour cells with low invasive potential. No collagenase secretion by tumour cells was ever detected. It is proposed that local TIMP levels may play a crucial in the control of tumour invasion in vivo.
Regulation of mopc 104e by T cells and growth factors induced by C. Parvum stimulation
Authors: Shrestha K. , Hiramoto R. N. , Ghanta V. K. 
Published: February 2007

AbstractCorynebacterium parvum, known for its ability to retard the growth of experimental neoplasms, was examined for its effect on the growth of murine plasmacytoma MOPC 104E in vivo and in vitro. Immunostimulation with C. parvum2 resulted in the accelerated growth of MOPC 104E in experimental animals, as measured by the tumor IgM production. The acceleration of plasmacytoma growth was seen in all cases where C. parvum was given before or at the time of tumor transplantation. Increased proliferation of MOPC 104E was also observed when MOPC 104E was co‐cultured in the presence of spleen cells from C. parvum stimulated mice as compared to the normal spleen cells. Removal of T cells by in vivo anti‐thymocyte serum treatment, followed by anti‐Thy 1.2 and complement in vitro, resulted in the partial loss of stimulatory activity. Furthermore, the stimulatory activity was shown to be associated with soluble mediators, which were generated by splenic adherent cells and T cells, and were, at least in part, responsible for the growth of plasmacytoma. Normal spleen cells did not generate a significant amount of soluble factor, but were able to augment MOPC 104E growth in co‐culture at high spleen to tumor cell ratio.
The impact of vaginal cytology on cervical cancer risks in cali, Colombia
Authors: Aristizabal Nubia , Cuello Carlos , Correa Pelayo , Collazos Tito , Haenszel William 
Published: February 2007

AbstractThe role of vaginal cytology screening in preventing invasive carcinoma of the cervix was investigated in Cali, Colombia. The history of previous participation in screening programs was obtained from 204 patients with invasive carcinoma and from 2 sets of age‐matched controls: one from the same health center and one for the same neighborhood of the patient. Our results show that the risk of developing invasive carcinoma is at least 10 times greater in non‐screened than in screened women.
Expression of laminin in thyroid gland and thyroid tumors: An immunohistologic study
Authors: Miettinen Markku , Virtanen Ismo 
Published: February 2007

AbstractThirty‐five thyroid tumors and four normal thyroids were immunohistochemically investigated for the presence of laminin, a major basement membrane component. The thyroid follicles in normal tissues and in nodular goiters were surrounded by a continuous rim of laminin positivity, as were also the papillae of papillary carcinomas. Follicular adenomas and well‐differentiated follicular carcinomas also showed laminin positivity around most of the follicles. Anaplastic thyroid carcinoma lacking a distinct compartmentalization of tumor cells also lacked pericellular laminin. However, in contrast to all other thyroid tumors, an intense laminin positivity was found in scattered tumor cells of anaplastic carcinomas. Laminin positivity is thus a feature reflecting the differentiation level of thyroid tumors, but, unlike most other carcinomas, differentiated thyroid tumors have basal laminae surrounding the differentiated structural units.
Interferon induction in human mixed leukocytetumor‐cell reactions: Evidence for restriction to a certain lineage expressing glycophorin A
Authors: Berger Rudolf , Knapp Walter , Kirchner Holger 
Published: February 2007

AbstractThis study reports the induction of interferon (IFN) in human mononuclear cells (MNC) by hematopoietic tumor cells. Only 3 out of 15 cell lines were capable of inducing IFN in the mixed leukocyte/tumor‐cell reaction (MLTR). K562, a pluripotent stem cell line and DUTKO‐1, a hybrid between K562 and Daudi (Burkitt lymphoma) induced high levels of antiviral activity (ranging from 50‐440 units IFN/ml). PUTKO‐I, a hybrid between K562 and P3HR‐1 (Burkitt lymphoma) induced very low levels of IFN (10 units/ml). This antiviral activity was produced by HLA‐DR+ adherent cells as revealed by different cell separation techniques, and shared well‐known properties of IFN γ (isoelectric point, inactivation by anti‐human IFN γ antibodies; species‐restricted protection). MLTR‐induced IFN induction could be blocked by enzyme treatment of tumor cells, but was still present when glutaraldehyde‐fixed cells were used for induction. Analysis of the cells by flow cytometry for expression of glycophorin A (GpA) revealed that expression of GpA correlated with the ability to induce antiviral activity in MLTR. Furthermore, isolated GpA could be used as a stimulant as well and the response to either K562 cells or soluble GpA was enhanced up to tenfold by the addition of a GpA‐specific monoclonal antibody.
A population‐based case‐control study of EBV and other viral antibodies among persons with Hodgkin's disease and their siblings
Authors: Evans Alfred S. , Gutensohn Nancy M. 
Published: February 2007

AbstractThe Epstein‐Barr virus (EBV) has been consistently found to be associated with Hodgkin's disease (HD) in two ways: cases generally have elevated titer distributions of antibodies against the viral capsid antigen, and the occurrence of HD among persons with a history of EBV infectious mononucleosis is two or three times higher than expected. We evaluated this association by measuring the prevalence and level of antibodies against EBV and related viruses among 304 cases of HD interviewed in a population‐based study in comparison to 285 of their siblings. The most significant finding was that antibody titers to the viral capsid antigen of EBV were elevated (± 1:320) in 39% of the cases and in only 14% of the sibling‐controls; the relative risk adjusted for age and sex was 4.1. The geometric mean titer was three‐fold higher among cases (175.6vs. 58.1) Subjects who reported a history of IM had a higher distribution of titers than those who did not. Cases also had elevated titers against the early antigen of EBV ‐ the D Component being most prominent. A significantly higher proportion of cases has elevated titers against CMV, relative risk = 3.4, but the prevalence of CMV antibody was relatively low and not consistently higher among cases. The findings support the hypothesis that EBV may play a role in the pathogenesis of HD among persons with elevated titers. The findings neither confirm nor deny a possible role of CMV.
Pregnancy in association with a newly diagnosed cancer: A population‐based epidemiologic assessment
Authors: Haas Joanna F. 
Published: February 2007

AbstractCancer patients who were pregnant at the time of cancer diagnosis were identified by the National Cancer Registry of the German Democratic Republic for the years 1970 through 1979. A total of 355 such cases occurred in women aged 15‐44, and during the same period 2,103,112 live births were registered. Rank by site in order of decreasing frequency was cervix, breast, ovary, lymphoma, melanoma, brain and leukemia. On the basis of general female population rates, 555.8 cases were expected, giving a significantly reduced observed to expected ratio (O/E) of 0.64. O/E ratios rose with age. The O/E for invasive carcinoma of the cervix was significantly elevated at 1.15; carcinoma in situ of the cervix occurred significantly less frequently than expected (O/E = 0.57). For breast, brain, melanoma and leukemia, significantly fewer cases were observed than expected. Explanations considered for the low number of pregnancy‐associated incident cancer cases include underreporting of pregnancy‐associated cancer, altered tumor progression in pregnancy or decreased fertility in women with early neoplastic disease.
Immune reactivity of the tumor‐resistant X/GF mouse
Authors: Nahas Fathallah , Segev Yardena , Gershon Harriet 
Published: February 2007

AbstractParameters of immune reactivity of the tumor‐resistant X/Gf mouse have been studied. X/Gf mice show better survival than do control strains following i.p. inoculation with graded doses of YAC lymphoma cells. The natural killer cell activity of X/Gf mice was higher than that of control mice, and this elevated NK activity was inherited by (C57BL/6 × X/Gf)F1 mice. Differences in NK activity of fresh spleen cells from X/Gf, C57BL/6, and (C57BL/6 × X/Gf)F1 mice were not reflected in their ability to lyse in vitro‐derived, cloned, transformed cell lines from X/Gf or C57BL/6 mice in an 8 h 51Cr assay; however, the X/Gf‐derived malignant cells were more sensitive to lysis than the C57BL/6‐derived cells. In order to test whether X/Gf mice demonstrated any exceptional ability to recognize and destroy altered autologous cells, experiments were performed with TNP‐modified syngeneic cells. While X/Gf and B10.A effector cells appeared to respond with similar magnitude, hapten‐modified X/Gf cells were stronger stimulator cells and more sensitive targets than control strain cells. The ability of X/Gf mice to reject H‐2 compatible and incompatible skin grafts is normal, as judged by a comparison of the rejection times with control strains. Allogeneic mixed lymphocyte reactivity, T‐cell‐mediated lympholysis, delayed‐type hypersensitivity reactions, and in vivo phagocytosis of inert particles are of comparable magnitude in X/Gf and control strain mice.
Further studies on the asymmetry of chromosome 15 duplication in trisomic leukemias of heterozygous origin: Preferential status of the AKR chromosome
Authors: Wirschubsky Zvi , Wiener Francis , Bregula Ursula , Klein George 
Published: February 2007

AbstractFour combinations of translocation heterozygotes with cytogenetically distinct chromosomes 15 were used to investigate whether the T‐cell leukemia‐associated preferential duplication of the AKR‐derived chromosome 15 (AKR‐15) is determined by factors within this chromosome, or is due to genes within the AKR genotype, but outside chromosome 15. Two of the four combinations were also used to determine whether the AKR‐15 duplication preference could be cancelled by MCF‐viremia in permissive F1 hybrids. Chemically and virally induced 15‐trisomic leukemias showed the same AKR‐15 duplication preference, which was due to some autonomous property of AKR‐15 itself. It was maintained in (C57BL 6;15 × C57BL) F1 leukemias, where 6;15 is the only AKR‐derived chromosome propagated on the C57BL/background. In the (C57BL 6;15 × AKR) F1 hybrid cross where both chromosomes 15 are of AKR origin, duplication occurred at random. To approach the second question, MCF viremia was induced by neonatal virus inoculation into permissive (AKR 6;15 × B6Fv‐1n) F1 hosts. The preferential duplication status of the AKR‐derived 6;15 remained unchanged.
Natural killer cell activity in women at “high risk” for breast cancer, with and without benign breast syndrome
Authors: Pross Hugh F. , Sterns Ernest , Macgillis Diane R. 
Published: February 2007

AbstractThis study is an analysis of natural killer cell (NK) function in 155 women repeatedly tested over a 5‐year period while attending breast screening clinics because of one or more of the following risk factors: family history breast cancer in a close female relative (relative risk = 1.2‐9); personal history ‐ early menarche, non‐parity, late menopause, etc (relative risk = 1.3‐3); clinical benign breast syndrome ‐ localized and diffuse (relative risk = 2‐4). Contrary to expectations, the high‐risk group as a whole had significantly higher than normal relative NK function vs K562 (1.21 ± 0.06 vs 1.00 ± 0.06) (p<0.02). Division into subgroups showed that the NK activities in patients with positive family histories, personal histories, or both, were exactly the same as normal values and that the increased NK function in the high‐risk group as a whole was due to those donors with benign breast syndrome (BBS). This group was also subdivided and the results were compared with the high‐risk patients with no BBS. The NK activity of the group having diffuse BBS (1.67 ± 0.05, n = 32) was significantly higher than that of the “No BBS” group (1.07 ± 0.07, n = 102) (p<0.025). A paired “ℓ”‐test performed on data from 7 patients who had no BBS and diffuse BBS at different times showed a significant difference of p<0.001, suggesting that the elevated NK activity is a reaction to the hormonal factors which cause this condition.
Evidence for extensive intermolecular disulfide bonds of the proteins of non‐ionic detergent high‐salt‐resistant skeletons of normal lymphocytes, and the altered structure in leukemia
Authors: Tas Sinan , Rodriguez Lewis V. , Drewinko Benjamin , Trujillo Jose M. 
Published: February 2007

AbstractNormal and leukemic lymphocytes were subjected to lysis with a non‐ionic detergent and 2.0 M NaCl. The intact nuclear DNA and the associated molecules resulting from these lyses (the nucleoids) were then separated from DNA‐dissociated molecules and analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Most of the proteins undissociable by 2.0 M NaCl from normal lymphocyte nuclear DNA or from DNA‐associated molecules appear to be intermolecularly S‐S linked. A distinct set of these proteins was either absent or only present in greatly decreased amounts in association with the leukemic lymphocyte nuclear DNA exposed to 2.0 M NaCl. On the other hand, the opposite relationship was found for a an MW 71,000 protein with leukemic lymphocytes of every patient studied regardless of the subtype of leukemia. Conversion of normal quiescent lymphocytes to cycling cells by lectin stimulation rendered them similar to leukemic cells in some respects but not in all. Our results show an altered nuclo(cyto)skeletal structure in leukemic cells; in general the proteins involved appear to be sulfhydryl proteins whose redox states and/or DNA association properties are modified by the neoplastic transformation of lymphocytes.
A case‐control study on lip cancer risk factors in ragusa (sicily)
Authors: Dardanoni Luigi , Gafà Lorenzo , Paternò Rosario , Pavone Giuseppina 
Published: February 2007

AbstractIncidence rates of lip cancer in males in Ragusa (Sicily) are amongst the highest in Europe [age‐standardized rate (world) for 1980‐82: 7.5 per 100,000]. A case‐control study was conducted on 53 male cases and 106 controls matched for sex, age (± 2 1/2 years), residence and hospital from which cases had been drawn. Individual interviews were carried out for the evaluation of ethnic, environmental, pathologic and occupational risk factors. Lip cancer was significantly associated with: fair, brown, or red hair (relative odds = 2.3), blue eyes (r.o. = 5.3), fair skin (r.o. = 8.0), sensitivity to sunburns (r.o. = 4.1), working outdoors (r.o. = 4.9), coexistence of non‐specific lesions of exposed body parts (r.o. = 12,2), low socioeconomic status (r.o. = 15.8), farming (r.o. = 2.6) and working in greenhouses (r.o. = 12.0). Recall of recurrent Herpes labialis was not significantly associated; also association with tobacco smoking was not significant after adjustment for socioeconomic status. Risk increased exponentially with the number of ethnic characteristics of northern European populations.
Paired epstein‐barr virus‐carrying lymphoma and lymphoblastoid cell lines Burkitt's lymphoma patients: Comparative sensitivity to non‐specific and to allo‐specific cytotoxic responses in vitro
Authors: Rooney C. M. , Rickinson A. B. , Moss D. J. , Lenoir G. M. , Epstein M. A. 
Published: February 2007

AbstractPaired Epstein‐Barr (EB) virus‐carrying cell lines have been established from Burkitt's lymphoma (BL) patients; one the BL‐cell line derived from the malignant cells of the tumour and bearing the relevant chromosomal translocation, the other the diploid lymphoblastoid cell line (LCL) derived from the patient's normal B cells by experimental infection with the virus. Comparative studies have shown that both BL and LCL cells are relatively resistant to fresh NK cells but are more susceptible to in vitro‐activated NK cells; individual pairs differ as to whether the BL or LCL cells are more sensitive to such effectors. With most cell pairs studied, the two cell types were equally efficient at inducing NK‐cell activation in vitro. When the in vitro stimulation protocol was changed to favour the induction of allo‐specific responses, most BL cells were noticeably poorer stimulators than the corresponding LCL although both cell types induced a similar pattern of cytotoxicity; this appeared to be directed against HLA class I allo‐antigens and could be inhibited by class I antigen‐specific monoclonal antibodies. Most BL target lines, though susceptible to this allo‐specific lysis, were significantly less so than the corresponding LCL. However, only 1/7 BL‐cell lines tested vis‐à‐vis the LCL gave evidence of a gross reduction in HLA antigen expression and this was immediately apparent from both serological typing and cellular analysis.
Strong natural killer (NK) cell activity in bone marrow of myeloma patients: Accelerated maturation of bone marrow NK cells and their interaction with other bone marrow cells
Authors: Uchida Atsushi , Yagita Masato , Sugiyama Hiroyuki , Hoshino Takashi , Moore Michael 
Published: February 2007

AbstractBone marrow natural killer (NK) cell activity was studied in patients with multiple myeloma. Bone marrow mononuclear cells from myeloma patients expressed considerable levels of cytotoxicity against K562 in a 4 h 51Cr‐release assay, which was comparable to that of blood lymphocytes. In contrast, NK‐cell activity was markedly low or absent in bone marrow of normal donors and control patients. Fractionation of bone marrow cells from myeloma patients on linear bovine serum albumin gradients enriched NK effector cells in the upper, lymphocyte‐enriched fraction, with no reactivity in the middle fraction containing mainly myeloma cells. Treatment with either Leu‐7 or OKMI monoclonal antibody plus complement reduced or abrogated bone marrow NK‐cell activity of myeloma patients as well as blood NK‐cell activity. However, OKMI plus complement failed to reduce control bone marrow NK‐cell activity, although the activity was reduced by Leu‐7 plus complement. Overnight exposure to interferon (IFN) of bone marrow cells resulted in an augmentation of NK‐cell activity in myeloma patients, but not in controls. Furthermore, adherent bone marrow cells from controls suppressed IFN‐induced enhancement of NK‐cell activity of blood lymphocytes, whereas bone marrow of myeloma patients did not contain such suppressor cells. No cytotoxicity was induced in control bone marrow cells by coculti‐vatton with bone marrow myeloma cells. Bone marrow NK cells from myeloma patients were able to lyse control bone marrow cells in a 18 h assay and their lytic activity was augmented by IFN treatment. However, neither bone marrow cells nor blood lymphocytes were cytotoxic to autologous and allogeneic fresh myeloma cells even after activation with IFN. These results suggest that NK precursor cells differentiate into HNK‐1‐and OKMI‐positive mature NK cells in bone marrow of myeloma patients, but not in control bone marrow, and that these functional bone marrow NK cells may interact with other bone marrow elements.
Primary liver cancer and oesophageal basal cell hyperplasia in rats caused by fusarium moniliforme
Authors: Marasas Walter F. O. , Kriek Nicolaas P. J. , Fincham John E. , Van Rensburg Schalk J. 
Published: February 2007

AbstractFusarium moniliforme Sheldon is one of the most prevalent fungi associated with maize throughout the world. A correlation has been found between the incidence of F. moniliforme in home‐grown maize and the human oesophageal cancer rate in Transkei, southern Africa. Culture material on maize of F. moniliformestrain MRC 826, isolated from maize in a high‐risk area for oesophageal cancer in Transkei, was either freeze‐dried or oven‐dried and fed to groups of 20 inbred male BDIX rats on a life‐long basis. At a dietary level of 8%, both types of culture material were hepatotoxic and caused 100% mortality. Hepatic lesions in rats that died were characterized by cirrhosis, nodular hyperplasia and bile‐duct proliferation. At a dietary level of 4% for 286 days followed by 2% for the remainder of the experiment, both types of culture material were hepatocarcinogenic and caused hepatocellular carcinoma in 80% and ductular carcinoma of the liver in 63% of the rats surviving more than 450 days. Only one of 30 such rats did not have a primary hepatic carcinoma. No hepatocellular or ductular carcinomas occurred in the controls. Hepatocellular carcinomas in the experimental rats invariably developed in severely cirrhotic livers showing nodular hyperplasia. Adenofibrosis also developed concurrently with hepatocellular carcinoma. A higher incidence of basal cell hyperplasia occurred in the oesophageal epithelia of rats fed freeze‐dried than in those fed oven‐dried material. The chemical nature of the hepatotoxic and hepatocarcinogenic mycotoxin(s) produced by F. moniliforme is unknown.
Biologic and biochemical differences between in vitro and in vivo passaged friend erythroleukemia cells. II. Changes in cell surface glycoproteins associated with a highly malignant phenotype
Authors: Amici C. , Ferrantini M. , Benedetto A. , Belardelli F. , Gresser I. 
Published: February 2007

AbstractFriend erythroleukemia cells (FLC), serially passaged in vkroor by intraperitoneal injection in DBA/2 mice, exhibit markedly different tumorigenicity and capacity to metastasize. We have attempted to determine whether the differences in tumorigenicity between these two lines of FLC were correlated with any biochemical changes in their cell membranes. Although consistent modifications of FLC membrane gangliosides were detected after FLC multiplied in the peritoneum, the pattern of FLC gangliosides was not a stable characteristic and did not correlate with tumorigenicity. In contrast, analysis of FLC membrane glycoproteins by cell surface labelling techniques (i.e., galactose‐oxidase‐borohydride techniques and polyacrylamide gel electrophoresis‐fluorography) or by metabolic labelling of glycoproteins with 3H‐galactose, revealed consistent differences in the high MW region of the gels between parental in vitro passaged FLC (either 745 or 3C1‐8 cells) and clones derived from in vivo passaged cells. No significant differences in the membrane proteins were detected between in vitro and in vivo passaged FLC when lactoperoxidase‐catalyzed iodination and polyacrylamide gel electrophoresis‐autoradiography were used. It is seen that repeated in vivo passages of FLC resulted in the appearance of different patterns of membrane glycoproteins and that these changes appeared to be associated consistently with the capacity of these cells to grow as tumor ascites and to metastasize to the liver and spleen.