bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Polymeric Nanoparticle to Co‐Deliver Mitochondria‐Targeting Peptides and Pt(IV) Prodrug: Toward High Loading Efficiency and Combination Efficacy
Authors: Yang Jinpeng , Yang Zhenlin , Wang Hua , Chang Yincheng , Xu Jiang‐Fei , Zhang Xi 
Published: February 2024

AbstractDeveloping combination chemotherapy systems with high drug loading efficiency at predetermined drug ratios to achieve a synergistic effect is important for cancer therapy. Herein, a polymeric dual‐drug nanoparticle composed of a Pt(IV) prodrug derived from oxaliplatin and a mitochondria‐targeting cytotoxic peptide is constructed through emulsion interfacial polymerization, which processes high drug loading efficiency and high biocompatibility. The depolymerization of polymeric dual‐drug nanoparticle and the activation of Pt prodrug can be effectively triggered by the acidic tumor environment extracellularly and the high levels of glutathione intracellularly in cancer cells, respectively. The utilization of mitochondria‐targeting peptide can inhibit ATP‐dependent processes including drug efflux and DNA damage repair. This leads to increased accumulation of Pt‐drugs within cancer cells. Eventually, the polymeric dual‐drug nanoparticle demonstrates appreciable antitumor effects on both cell line derived and patient derived xenograft lung cancer model. It is highly anticipated that the polymeric dual‐or multi‐drug systems can be applied for combination chemotherapy to achieve enhanced anticancer activity and reduced side effects.
Monitoring the threatened Harttiella (Siluriformes, Loricariidae) with environmental DNA: A comparison between metabarcoding and targeted digital PCR
Authors: Condachou Céline , Cuenot Yves , Pigeyre Laetitia , Covain Raphael , Brosse Sébastien , Murienne Jérôme 
Published: February 2024

Abstract
Environmental DNA has emerged as an efficient alternative to traditional sampling methods. A standard multispecies or a targeted single‐species approach can be used for analysing environmental DNA samples. The costs, benefits, and drawbacks associated with these two approaches are quite different.
Here, a comparison between standard multispecies metabarcoding and targeted species assay using digital PCR (dPCR) for two threatened Harttiella species occurring in French Guiana (Harttiella lucifer and H. nsp. “Makwali”) was performed.
Samples were collected in 11 sites of the upper Maroni River drainage basin and located in the Galbao mountain range, known to host the targeted species. The “Teleo” primer was used for the metabarcoding approach. To implement a new dPCR assay, specific primers and probes for the two targeted Harttiella species were developed.
This targeted dPCR assay detected Harttiella in seven sites. All of these sites were also positive for metabarcoding detection, and the habitat characteristics were favourable for the species.
This study demonstrated that both targeted (dPCR) and multispecies (metabarcoding) approaches can be used for the monitoring of the threatened H. lucifer and H. nsp. “Makwali” species.
From a conservation point of view, we recommend to use the metabarcoding approach to update the spatial distribution of the species, while the dPCR method can be used for a temporal follow‐up of known Harttiella populations.
Solid‐State Emissive Pillar[6]arene Derivative Having Alternate Methylene and Nitrogen Bridges
Authors: Ohtani Shunsuke , Nakaguchi Kazeto , Kato Kenichi , Ogoshi Tomoki 
Published: February 2024

AbstractMacrocyclic arenes show conformational adaptability, which allows host–guest complexations with the size‐matched guest molecules. However, their emission properties are often poor in the solid states due to the self‐absorption. Herein, we newly synthesized pillar[6]arene derivatives having alternate methylene and nitrogen bridging structures. Solvatochromic study reveals that the nitrogen‐embedding into the cyclic structures can strengthen the intramolecular charge transfer (CT) nature compared to that of the linear nitrogen‐bridged precursor. Owing to the large Stokes shift in the solid state, one of the nitrogen‐embedded pillar[6]arenes shows high absolute photoluminescence quantum yield (ΦPL=0.36). Furthermore, it displays a turn‐off sensing ability toward nitrobenzene (NB) vapor; a fluorescence quenching is observed when exposed to the NB vapor. From the structural analysis before and after the exposure of NB vapor, the amorphous nitrogen‐embedded pillar[6]arene efficiently co‐crystallize with NB and formed non‐emissive intermolecular CT complexes with NB.
Small Molecule SHP2 Inhibitor LXQ‐217 Affects Lung Cancer Cell Proliferation in Vitro and in Vivo
Authors: Sun Hao , Bai Xiaoyi , Zhang Yiting , Gao Yanan , Dai Jiajia , Xing Pan , Zhu Jiqiang , Liu Ruihua , Wang Zemin , Li Xiangqian 
Published: February 2024

AbstractBackground: SHP2 is highly expressed in a variety of cancer and has emerged as a potential target for cancer therapeutic agents. The identification of uncharged pTyr mimics is an important direction for the development of SHP2 orthosteric inhibitors.Methods: Surface plasmon resonance analysis and cellular thermal shift assay were employed to verify the direct binding of LXQ‐217 to SHP2. The inhibitory effect of LXQ‐217 was characterized by linear Weaver‐Burke enzyme kinetic analysis and BIOVIA Discovery Studio. The inhibition of tumor cell proliferation by LXQ‐217 was characterized by cell viability assay, colony formation assays and hoechst 33258 staining. The inhibition of lung cancer proliferation in vivo was studied in nude mice after oral administration of LXQ‐217.Results: An electroneutral bromophenol derivative, LXQ‐217, was identified as a competitive SHP2 inhibitor. LXQ‐217 induced apoptosis and inhibited growth of human pulmonary epithelial cells by affecting the RAS‐ERK and PI3 K‐AKT signaling pathways. Long‐term oral administration of LXQ‐217 significantly inhibited the proliferation ability of lung cancer cells in nude mice. Moreover, mice administered LXQ‐217 orally at high doses exhibited no mortality or significant changes in vital signs.Conclusions: Our findings on the uncharged orthosteric inhibitor provide a foundation for further development of a safe and effective anti‐lung cancer drug.
Substitution Lability of the Perfluorinated Cp* Ligand in [Rh(COD)(C5(CF3)5)] Towards Triphenylpnictogens EPh3 (E=N, P, As, Sb, Bi)
Authors: Kub Nico G. , Sievers Robin , Parche Joshua , Malischewski Moritz 
Published: February 2024

AbstractTriphenylpnictogens EPh3 (E=N, P, As, Sb, Bi) are able to displace the perfluorinated Cp* ligand in [Rh(COD)(C5(CF3)5)] (COD=1,5‐cyclooctadiene) in up to quantitative yield. The resulting ionic products contain [C5(CF3)5]− as uncoordinated counter anion. The cations feature [Rh(COD)]+ fragments, coordinated by one (N, Bi), two (P, As) or three (Sb) triphenylpnictogen moieties. Whereas coordination via the pnictogen is observed for P, As and Sb, π‐coordination of the aryl rings is observed for N and Bi.
Censored autoregressive regression models with Student‐t innovations
Authors: Valeriano Katherine A. L. , Schumacher Fernanda L. , Galarza Christian E. , Matos Larissa A. 
Published: February 2024

AbstractData collected over time are common in applications and may contain censored or missing observations, making it difficult to use standard statistical procedures. This article proposes an algorithm to estimate the parameters of a censored linear regression model with errors serially correlated and innovations following a Student‐ distribution. This distribution is widely used in the statistical modelling of data containing outliers because its longer‐than‐normal tails provide a robust approach to handling such data. The maximum likelihood estimates of the proposed model are obtained through a stochastic approximation of the EM algorithm. The methods are applied to an environmental dataset regarding ammonia‐nitrogen concentration, which is subject to a limit of detection (left censoring) and contains missing observations. Additionally, two simulation studies are conducted to examine the asymptotic properties of the estimates and the robustness of the model. The proposed algorithm and methods are implemented in the R package ARCensReg.
Long‐term drought promotes invasive species by reducing wildfire severity
Authors: Kimball Sarah , Rath Jessica , Coffey Julie E. , Perea‐Vega Moises R. , Walsh Matthew , Fiore Nicole M. , Ta Priscilla M. , Schmidt Katharina T. , Goulden Michael L. , Allison Steven D. 
Published: February 2024

AbstractAnthropogenic climate change has increased the frequency of drought, wildfire, and invasions of non‐native species. Although high‐severity fires linked to drought can inhibit recovery of native vegetation in forested ecosystems, it remains unclear how drought impacts the recovery of other plant communities following wildfire. We leveraged an existing rainfall manipulation experiment to test the hypothesis that reduced precipitation, fuel load, and fire severity convert plant community composition from native shrubs to invasive grasses in a Southern California coastal sage scrub system. We measured community composition before and after the 2020 Silverado wildfire in plots with three rainfall treatments. Drought reduced fuel load and vegetation cover, which reduced fire severity. Native shrubs had greater prefire cover in added water plots compared to reduced water plots. Native cover was lower and invasive cover was higher in postfire reduced water plots compared to postfire added and ambient water plots. Our results demonstrate the importance of fuel load on fire severity and plant community composition on an ecosystem scale. Management should focus on reducing fire frequency and removing invasive species to maintain the resilience of coastal sage scrub communities facing drought. In these communities, controlled burns are not recommended as they promote invasive plants.
The Green Synthesis of 2‐Pyrrolin‐5‐ones from Bio‐renewable Glyoxal, Diketones and Amines
Authors: Oyejobi Aanuoluwapo O. , Gao Dandan , Wang Jiahua , Tang Xiang‐Ying , Wang Long 
Published: February 2024

AbstractDescribed herein is an effective and green reaction between diketones, amines and bio‐renewable glyoxal, affording a wide range of 2‐pyrrolin‐5‐ones and releasing water as the only byproduct. The reaction features step‐economy, high efficiency, mild conditions, broad substrate scope, hazardous waste avoiding and easy scale‐up. The further transformation between the products and aldehydes can be potentially applied for fluorescent dye synthesis.
A flexible ammonia sensor based on MXene membrane with high sensitivity
Authors: Zhao Qian , Li Yilin , Zhang Zhouxiaolong , Dai Li , Liu Xuhan , Sun Yanhui , Zhao Dan , Zhu Nan 
Published: February 2024

AbstractAmmonia (NH3) of high concentration will pose a threat to ecological environment or human health, and exhaled NH3 is significant in disease monitoring and diagnosis. Thus, developing a highly sensitive gas sensor is significant to monitor NH3 concentration in complex environments. However, traditional NH3 sensors either need high working temperature, or face the challenge of poor conductivity/ sensitivity. In this work, NH3 sensors based on self‐assembled MXene membrane have been fabricated. As‐prepared sensors show a high sensitivity of 2.10 ppm−1 towards extremely low concentrations of NH3 (ppb level) at room temperature, attributed to large surface area and high conductivity. In addition, the sensors also display low detection limit (50 ppb), fast response time (41 s), good recoverability, long‐term stability (15 days) and excellent flexibility (1000 bending cycles) towards NH3. The results provide insights into the development of highly sensitive NH3 sensors for industrial or biomedical applications.
Importance of higher modes for dynamic soil structure interaction of monopile‐supported offshore wind turbines
Authors: Sah Upendra Kumar , Yang Jun 
Published: February 2024

AbstractOffshore wind turbines (OWTs) have emerged as one of the most sustainable and renewable sources of energy. The size of OWTs has been increasing, which creates more challenges in the design of foundations due to the potential higher‐mode effects involved in the dynamic soil‐structure interaction (DSSI) response. Several foundation modeling techniques are available for calculating the OWT fundamental frequency; however, their capability to predict the higher modes by considering real geometric configurations is unclear. The main aim of this study is to perform a rigorous modal analysis of the NREL 5MW reference OWT to investigate the higher mode effects using the 3D finite element method. A detailed parametric analysis is also performed to study the effects of soil inhomogeneity, initial soil modulus, and the monopile dimensions (diameter, thickness, and embedded pile depth) on higher modes' natural frequencies and effective mass participation ratios. The study shows that dynamic soil‐structure interaction has a significant role in modal response and the simplified foundation models are not accurate enough. Given the significant contribution from higher modes, they should not be simply ignored in the OWT design, particularly in earthquake‐prone zones.
Tail risk forecasting and its application to margin requirements in the commodity futures market
Authors: Feng Yun , Hou Weijie , Song Yuping 
Published: February 2024

AbstractThis study presents a dynamic analysis framework called autoregressive conditional extreme value (AEV), designed for modeling the daily maximum drawdowns of commodity futures markets, using steel rebar futures as an illustrative example. The research demonstrates that AEV outperforms AR or generalized autoregressive conditional heteroskedasticity (GARCH)‐type benchmark models in terms of in‐sample fitting and out‐of‐sample forecasting accuracy. Notably, AEV's time‐varying shape parameter (tail index) sensitively captures the clustering nature of tail risk and differentiates between long‐ and short‐side markets. The study also presents theoretical findings regarding AEV‐based value at risk (VaR) and expected shortfall (ES), and empirically measures and predicts the tail risk of the steel rebar futures market. Moreover, the research extends the methodology to create a dynamic margin model for Chinese commodity futures, showing that the AEV‐based model effectively achieves the specified risk coverage targets and significantly reduces current exchange margin requirements.
The effect of stir‐frying on the aging of oat flour during storage: A study based on lipidomics
Authors: Zhang Yuanyuan , Sun Minjun , Huo Rui , Gu Qixin , Miao Ying , Zhang Meili 
Published: February 2024

AbstractIn this study, we used the LC‐ESI‐MS/MS technique to elucidate the effects of stir‐frying on the lipidomics of oat flour before and after storage. We detected 1540 lipids in 54 subclasses; triglycerides were the most abundant, followed by diacylglycerol, ceramide (Cer), digalactosyldiacylglycerol, cardiolipin, and phosphatidylcholine. Principal component analysis and orthogonal least squares discriminant analysis analyses showed that oat flour lipids were significantly different before and after storage in stir‐fried oat flour and raw oat flour. After oat flour was stir‐fried, most of the lipid metabolites in it were significantly downregulated, and the changes in lipids during storage were reduced. Sphingolipid metabolism and ether lipid metabolism were the key metabolic pathways, and Cer, PC, and lyso‐phosphatidylcholine were the key lipid metabolites identified in the related metabolic pathways during oat flour storage. Frying inhibits lipid metabolic pathways during storage of oat flour, thereby improving lipid stability and quality during storage. This study laid the foundation for further investigating quality control and the mechanism of changes in lipids during the storage of oat flour.
Quality improvement of oil extracted from flaxseeds (Linum usitatissimum L.) incorporated with olive leaves by cold press
Authors: Teimouri Okhchlar Ramin , Javadi Afshin , Azadmard‐Damirchi Sodeif , Torbati Mohammadali 
Published: February 2024

AbstractFlaxseed oil has a high amount of α‐linolenic acid (an ω3 essential fatty acid), but it is very prone to oxidation. Therefore, olive leaves were used as a rich source of phenolic compounds with flaxseeds upon oil extraction by cold press to enhance the oxidative stability of extracted oils. Oil from flaxseeds with unblanched leaves and blanched leaves at level of (0 [control sample], 2.5, 5, 7.5, and 10% w/w) was extracted by cold press. Quality of extracted oils was evaluated for 90 days of storage at room condition. Incorporation of unblanched olive leaves could increase the acid value of the extracted oils up to 2.0 (mg KOH/g oil) compared to the other samples. Oxidation of the flaxseed oil could be delayed by the addition of blanched olive leaves up to 5%. Oil extracted from flaxseeds incorporated with blanched olive leaves had higher content of carotenoids (up to 33.7 mg/kg oil), chlorophylls (up to 35.7 mg/kg oil), and phenolic compounds (up to 200 mg/kg oil). Also, oxidative stability of extracted oils was higher up to 7.5% of blanched olive leaves (11.4 h) compared to control sample (7.2 h) and other oil samples. Polyunsaturated fatty acids of the oil samples were well preserved by the incorporation of blanched olive leaves. Based on the obtained results, incorporation of suitable amount of blanched olive leaves (up to 7.5%) with flaxseeds before oil extraction by press can be an appropriate procedure to produce oils with high content of bioactive components and suitable oxidative stability.
CD30 expression in an emerging group of mesenchymal spindle cell neoplasms with ALK fusion detected by flow cytometry and immunohistochemistry
Authors: Iwakoshi Akari , Kikui Hajime , Nakashima Ryosuke , Goto Yuya , Ichikawa Daisuke , Sasaki Eiichi , Sekimizu Masahiro , Hattori Hiroyoshi , Maeda Naoko 
Published: February 2024

AbstractAn emerging group of spindle cell neoplasms harboring fusions involving NTRK or non‐NTRK kinase genes often share characteristic S100 and/or CD34 expression; however, the diagnostic utility of immunohistochemical stains is not well established in this family owing to their lack of specificity. Recently, CD30 expression in spindle cell neoplasms with kinase gene fusions, such as NTRK, BRAF, RAF1, and RET, has been increasingly identified. We herein report a 10‐year‐old girl with high‐grade spindle cell sarcoma of the neck. Prior to histopathological evaluation, flow cytometry (FCM) analysis and touch smear cytology of the tumor tissue revealed CD34+ and dimCD30+ spindle cell populations. Histopathologically, the case was characterized by monomorphic spindle‐shaped cytomorphology with CD30, S100, and CD34 positivity and harbored close similarities with spindle cell neoplasms with NTRK or non‐NTRK gene fusions. Subsequently, a comprehensive next‐generation sequencing sarcoma panel identified a rare PLEKHH2::ALK fusion, and a diagnosis of ALK‐rearranged spindle cell neoplasm was made. The patient showed significant tumor response to single‐agent treatment with alectinib, an ALK‐tyrosine kinase inhibitor. This case supports that CD30 is expressed in an ALK‐rearranged mesenchymal neoplasm. The benefit of the early detection of CD30 expression by FCM for a prompt diagnosis and treatment is highlighted in the context of an aggressive clinical course. This case represents a learning experience regarding the need to the check the status of CD30 expression in these tumors and suggests the potential clinical benefits of CD30‐targeted therapy.
Development of a head and neck lymphoedema specific quality of life tool: The Comprehensive Assessment of Lymphoedema Impact in the Head and Neck
Authors: Starmer Heather M. , Patterson Joanne , Young Bridget , Fleming Jason , Cherry Mary Gemma 
Published: February 2024

AbstractPurposeTo develop a head and neck lymphoedema (HNL) specific quality of life (QoL) instrument to assess physical, functional, and social/emotional impacts of HNL.MethodsInstrument candidate items were reviewed by patients with HNL and clinicians and rated for importance, clarity, and invasiveness. The Content Validity Ratio was applied for item reduction. Three‐step cognitive interviews were conducted with HNL patients to validate the items, survey format, and instructions.ResultsInitially, 130 candidate questions were developed. Following item reduction, 52 items progressed to three‐step cognitive interviews. Following cognitive interviews, the Comprehensive Assessment of Lymphoedema Impact in Head and Neck (CALI‐HaN) included 33 items; 1 global, 10 physical, 7 functional, and 15 emotional.ConclusionsPhysical, functional, and socioemotional effects need to be considered when measuring QoL in patients with HNL. This study describes initial development of the CALI‐HaN, an instrument that shows promise for clinical and research applications following future validation.
Parental practices and perspectives on health and digital technology use information seeking for children aged 0–36 months
Authors: Hendry Danica , Straker Leon , Bourne Brittany , Coshan Sarah , Kumwembe Ndapile , McCarthy Caroline , Zabatiero Juliana 
Published: February 2024

AbstractBackgroundParents commonly seek information to support the health and well‐being of their children. The increasing availability of health information online and social changes related to the COVID‐19 pandemic may have changed what information is sought, from whom, where, and why. This qualitative study explored parents' practices and perspectives on seeking health and digital technology use information for their young children.MethodsTwenty parents, living in Australia (7 rural, 3 remote, and 10 metropolitan), with children aged 0–36 months completed a semi‐structured interview.ResultsParents commonly turned to friends and family and online sources to access health information for their young children. For all types of health information, including digital technology use, themes were identified surrounding aspects of information sources participants valued and accessibility of health services. Perceived credibility and trustworthiness, relatability with other parents, ease of accessibility and convenience, and actionable, bite‐sized information were valued. Reduced accessibility to health services due to COVID‐19 and geographical location, and need for agency in managing their child's health influenced parents' choice of source of information. Few participants actively sought information about digital technology use for their young child, with the main focus on screen time.ConclusionInteractions with family and friends and online sources are important to parents when accessing health information for their child. Parents valued information sources which they considered trustworthy, credible, and relatable, as well as easily accessible and convenient.So What?Dissemination of health information reflecting these values may empower parents during this early stage of parenthood.
The role of task preference in the effectiveness of response interruption and redirection
Authors: Sloman Kimberly N. , Torres‐Viso Mariana , Edelstein Matthew L. , Schulman Rebecca K. 
Published: February 2024

AbstractResponse interruption and redirection (RIRD) is a common treatment for automatically reinforced vocal stereotypy; it involves the contingent presentation of task instructions. Tasks that are included in RIRD are typically selected based on caregiver report, which may affect the efficacy of RIRD. The purpose of the current study was to evaluate the role of task preference in the efficacy of RIRD for four participants who engaged in vocal stereotypy. We conducted task‐preference assessments and selected tasks of varying preferences to include in RIRD. For three out of four participants, the results showed that RIRD with higher preference tasks was not effective at reducing vocal stereotypy, whereas RIRD with lower preference tasks was effective for all participants.