bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of miRNAs miR-125a-5p, -27a-5p, -193a-5p, -135b-5p, -451a, -495-3p and -136-5p in parental ovarian cancer cells and secreted extracellular vesicles
Authors: Skryabin G. O. , Beliaeva A. A. , Enikeev A. D. , Bagrov D. V. , Keremet A. M. , Komelkov А. V. , Elkin D. S. , Sylantieva D. M. , Tchevkina E. M. 
Published: April 2024

Introduction. The identification of markers for liquid diagnostics of ovarian cancer is one of the most urgent tasks of gynecologic oncology. Currently, extracellular vesicles (EVs) are of great interest as a source of oncomarkers, including miRNA markers. We have previously shown that the levels of miR-125a-5p, -27a-5p, -193a-5p and 135b-5p are significantly elevated and miR-451a, -495-3p and -136-5p are significantly decreased in the EVs from uterine aspirates of ovarian cancer patients.Aim. Analysis of miR-125a-5p, -27a-5p, -193a-5p, 135b-5p, 451a, 495-3p and -136-5p levels in ovarian cancer cell cultures and secreted EVs.Material and methods. Cultivation of ovarian cancer cell lines: OVCAR-3, OVCAR-4, OVCAR-8 and SKOV3; EVs isolation from conditioned medium by ultracentrifugation; EVs validation by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), western blot analysis of exosomal markers; isolation of miRNAs from cells and EVs; analysis of miRNAs by Stem-Loop – reverse transcription-quantitative polymerase chain reaction.Results. In all cell lines studied, the expression of miR-125a-5p, -27a-5p, -193a-5p and -135b-5p significantly exceeds the expression of -451a, -495-3p and -136-5p. All ovarian cancer cell lines are featured by a “cells >EVs” ratio for highly expressed miRNAs and “EVs >cells” ratio for poorly expressed miRNAs.Conclusion. The results of the study support the relation between the differential expression of studied miRNAs and the pathogenesis of ovarian cancer and confirm the high diagnostic potential of these molecules.
Transcriptional, growth factors, components of the AKT/mTOR signaling pathway, receptors and ligands of programmed cell death expression in melanoma
Authors: Nikulnikov K. V. , Bogdanova V. A. , Spirina L. V. , Chizhevskaya S. Yu. , Kondakova I. V. , Choynzonov E. L. , Chernov V. I. 
Published: April 2024

Introduction. Melanoma is the most dangerous neoplasm of the skin, characterized by a malignant and aggressive course. Transcriptional and growth factors, components of the AKT/mTOR signaling pathway, receptors and ligands of programmed cell death are involved in significant processes of oncogenesis.Aim. To study the expression of components of the AKT/mTOR (mTOR – mammalian target of rapamycin) signaling pathway, transcription and growth factors, expression of AMPK, LC3B, programmed cell death 1 (PD-1), programmed death-ligand 1 PD-L1 and programmed death-ligand 2 (PD-L2) in skin and mucosal tumor tissues.Materials and methods. The study included 21 patients with a verified diagnosis of melanoma of the skin of various localizations and mucous membranes of the nasal cavity T1a–4bN0M0 (I–IV stages) and 18 patients with basal cell carcinoma of the skin of various localizations T1–4N0M0 (I–VIA stages), aged 45 to 72 years old, who were treated in the department of head and neck tumors of the Cancer Research Institute, Tomsk National Research Medical Center. The presence of tumor ulceration was determined by microscopy and registration of the true absence of the epidermis over the tumor or due to traumatization of the epidermis. Expression of components of the AKT/mTOR signaling pathway, transcription and growth factors, expression of AMPK, LC3B, PD-1, PD-L1 and PD-L2 in the tumor tissue was determined by real-time polymerase chain reaction.Results. An increase in the expression of 70 S6 kinase and VHL was found in melanoma tissues compared to basal cell carcinoma. At the same time, the presence of signs of ulceration was associated with a low level of c-RAF, nuclear factor kappa B (NF-kB) p50 and hypoxia-inducible factor 1 (HIF-1) matrix RNA (mRNA) against the background of an increase in the expression of the hypoxia-inducible factor 2 (HIF-2) transcription factor. The study of the molecular features of neoplasms in relation to the tumor thickness according to Breslow revealed the contribution of transcription and growth factors, the intensity of intracellular signaling processes, modification of the microenvironment, autophagy and neoangiogenesis.Conclusion. The molecular and biological features of melanomas associated with invasive tumor growth have been identified. An increase in the expression of 70 S6 kinase and VHL are characteristic of a malignant skin tumor. The presence of signs of ulceration and tumor invasion were associated with a change in the transcriptional characteristics of factors with the induction of key markers, oncogenesis, which contributes to the formation of the invasive potential of the tumor.
Dynamics of miRNA expression in urine extracellular vesicles of prostate cancer patients after radical prostatectomy
Authors: Shutko E. V. , Bryzgunova O. E. , Ostal’cev I. A. , Pak S. V. , Krasi’nikov S. E. , Laktionov P. P. , Konoshenko M. Yu. 
Published: April 2024

Introduction. It is known that the treatment of oncological diseases including prostate cancer (PCa) causes changes in the expression of oncogenic and oncosuppressive miRNAs. The analysis of miRNA expression dynamics can be used to predict the course of the disease and its response to therapy. However, the effect of PCa therapy on the expression of extracellular miRNAs is just beginning to be investigated.Aim. To study the expression dynamics of 14 miRNAs (miR-19b, -22-3p, -30e, -31, -92a, -125b, -144, -200b, -205, -222, -375, -378a, -425, -660) in urine extracellular vesicles of PCa patients after radical prostatectomy and to reveal prognostic miRNA ratios.Materials and methods. Urine samples of 18 donors and 18 PCa patients, obtained before radical prostatectomy, 1 week and 3 months after surgery, were examined. Extracellular vesicles were isolated by aggregation-precipitation protocol; extracellular vesicles miRNAs were isolated using fiberglass sorbents and octane acid. Data on threshold detection cycles of 14 miRNAs were obtained using reverse transcription – loop polymerase chain reaction (TaqMan).Results. It was found that prostatectomy causes a significant change in the relative expression of 44 miRNA ratios in the urine of PCa patients. Four groups of miRNA ratios can be distinguished: 1) miRNA ratios, which expression level significantly differed between donors and PCa patients before surgery and significantly changed in PCa patients 3 months after prostatectomy in the direction of the level of donors (6 pairs); 2) miRNA ratios, which expression did not significantly differ between donors and PCa patients before surgery, but significantly differed from the baseline in PCa patients and donors 3 months after prostatectomy (5 pairs); 3) miRNA ratios, based on expression ratios of which PCa patients can be divided into two or three significantly different subgroups 3 months after prostatectomy (19 pairs); 4) miRNA ratios that did not significantly change their expression after prostatectomy (30 pairs).Conclusion. Prostatectomy causes a significant change in the level of expression of miRNA in urine. 6 pairs of miRNAs, the relative expression of which after surgery significantly changed towards that of healthy donors and 19 pairs of miRNAs, according to the level of relative expression of which patients with prostate cancer were divided into two significantly different subgroups 3 months after prostatectomy, were identified based on the analysis of the dynamics of miRNA expression after prostatectomy.
The role of ABC-transporters in homeostasis, cancer pathogenesis and therapy
Authors: Boichuk S. V. , Ivoilova T. V. 
Published: April 2024

ABC transporters (ATP Binding Cassette (ABC) transporters) are proteins, which play a dual role in the substances transport across the membrane. On the one hand, they transport nutrients and other molecules inside the cell to supply the necessary nutrients, on the other hand, these proteins excrete some endogenous and exogenous substrates from the cell to maintain their homeostasis in the body and prevent from effects of aggressive environment. ABC transporters play a role in the pathogenesis of various metabolic disorders. In addition, a large amount of evidence has been accumulated about the participation of these proteins in oncogenesis because of their involvement into initiation, progression, invasion and metastasis of tumors, as well as development of multidrug resistance phenotype. Currently, these proteins are attractive therapeutic targets, influence on which can significantly increase the effectiveness of anticancer therapy and improve the prognosis of patients with oncological diseases, including recurrent, metastatic and inoperable forms.The review provides information on drugs that affect the functional activity of ABC transporters and the mechanisms of their action, and also presents the results of clinical trials of these inhibitors.
The role of circulating neutrophils in the progression of kidney cancer
Authors: Magdieva I. R. , Abakumova T. V. , Dolgova D. R. , Gorshkov O. Y. , Gening T. P. 
Published: April 2024

Introduction. Currently, the question of the role of neutrophils in the progression of kidney cancer remains relevant. Neutrophils are capable of exhibiting protumor properties through the secretion of cytokines, chemokines, and growth factors, which is determined by the expression of genes for these molecules. And the functional heterogeneity of neutrophils is characterized by differences in gene expression patterns.Aim. To assess the role of circulating neutrophils in the progression of kidney cancer.Materials and methods. In circulating neutrophils of patients with verified clear cell kidney cancer at stages I–III according to Tumor, Nodus and Metastasis (TNM) (n = 88) before surgical treatment and conditionally healthy donors (control group) (n = 20), the expression of NGAL genes was determined using quantitative reverse transcription polymerase chain reaction, MMP-13 and VEGF-A.Results. There was an increase in NGAL gene expression in circulating neutrophils (p = 0.05) at the initial stage and a decrease in it at advanced stages of kidney cancer (p = 0.03). High expression of the MMP-13 gene by circulating neutrophils was detected at all stages of kidney cancer relative to control values (at stage I p = 0.005; at stage II p = 0.003; at stage III p = 0.0008). A significant direct correlation was observed between the expression of the NGAL and MMP-13 genes in neutrophils at stage I kidney cancer (r = 0.696; p = 0.003). In the group of patients with kidney cancer, a direct correlation was found between the expression of the NGAL and VEGF-A genes (r = 0.322; p = 0.049). A multivariable Cox regression model for disease-free survival revealed the predictive value of VEGF-A and NGAL genes expression in circulating neutrophils. With an increase in the expression of the VEGF-A and NGAL genes in neutrophils by 1 unit, the risk of metastases increases by 0.80 (0.65–0.99; p = 0.043) and 1.42 (1.01–2.00; p = 0.046) times, respectively. The Kaplan–Meier analysis of disease-free survival in patients with kidney cancer showed the influence of NGAL expression in circulating neutrophils on progression-free time. In the group of patients with high NGAL expression, the median follow-up was 31.7 months, and in the group with low NGAL expression – more than 36 months (log-rank-test; p = 0.017).Conclusion. Thus, the data obtained suggest that circulating neutrophils play a leading role in the progression of kidney cancer. The level of expression of NGAL in circulating neutrophils can be used to predict the relapse-free period in patients with kidney cancer.
Le sentiment dans les Pensées de Pascal: son origine, ses fonctions, son statut
Authors: McKenna Antony 
Published: April 2024

Pascal founds his interpretation of the Augustinian doctrine of the corruption of human nature on a philosophy of faith inherited from Montaigne: « we are Christians in just the same way as we are Périgordians or Germans » (Essais, II, 12) : this conception of « human faith » is analysed, in turn, by means of concepts drawn from Descartes (passion) and Gassendi (imagination). He thus leads us to a very modern conception of « human faith » – without grace – as being the product of education and habit, a « sentiment » like so many others.
Study of artificial intelligence and its awareness in dental mainly aesthetic dentistry and prosthodontics
Authors: Rastogi Isha 
Published: April 2024

: Each day there is a new research. Similarly in dental profession with recent advances, artificial intelligence is a new trend. It's for better accurate superior health care. This makes dental diagnosis treatment plan better and successful. : This study is to assess the awareness and knowledge of artificial intelligence in dental profession. : this cross sectional study was done by questionnaire in 100 dental professionals. This was to know their knowledge and awareness in artificial intelligence. : very few dental professionals know of artificially intelligence. Those who know actually know v little. : Attempt should be made to know more about artificial intelligence. Its new application and more uses of artificial intelligence can be done in dental in future.
Canalicualr adenoma in buccal space area : a case report
Authors: Alwakeel Abdulaziz , Shiekh Musa Asma 
Published: April 2024

Introduction: Canalicular adenoma (CA) is considered a rare benign salivary gland neoplasm, although it was suggested that this lesion has a terminal duct origin. The most common location of this lesion is the upper lip which accounted for 80% of cases, followed by buccal mucosa and palatal area, it presented as a solitary lesion in middle age more in female patients.
Case presentation: 75 years old, male, medically fit referred to the oral medicine and pathology clinic because he complaint of right cheek swelling for several years associated with discomfort but no associated pain or increase in the size of the lesion that presented in his cheek, the patient clinical examination revealed hard well-demarcated lesion in buccal area extended up to the zygomatic bone area, lesion can be palpable by using bi digest technique, CT x-ray was done for patient and shows two cystic lesions in right buccal space area, excisional biopsy done and histopathological evaluation revealed the diagnosis was Canalicular adenoma
Conclusion: Canalicular adenoma is a benign salivary gland neoplasm that can be presented as solitary or multiple but solitary type more common, usually effect the upper lip and more in female patients with middle age, In our case the lesion presented in buccal mucosa area in male patient above 70 years old, clinical and histopathological evaluation is important to reach the diagnosis, clinical follow up is recommended in such lesion because there is a chance for recurrences
Transcreating Sanskrit Humour through Kūṭiyāṭṭam: the Translation and Performance of the Rasasadana Bhāṇa
Authors: Mucciarelli Elena , Sathaye Adheesh 
Published: April 2024

This essay develops a collaborative, performance-oriented approach to the task of translating a nineteenth-century Sanskrit play, the Rasasadana Bhāṇa of Godavarma Yuvaraja. The bhāṇa is a genre of Sanskrit comedic monologue, with examples dating from the late Gupta period (c. 500 ce), that has had a significant but largely overlooked presence in Kerala since the fourteenth century. To better understand this presence, and to explore the potential of performance for aiding in translation practice we partnered with the Nepathya Centre for Excellence (Moozhikkulam, Kerala) to produce digital “transcreations” of select verses from the Rasasadana performed in a Kūṭiyāṭṭam style. This essay outlines our methodology and presents key outcomes from a series of collaborative research sessions conducted remotely during the COVID-19 pandemic. We assess how the juxtaposition of English translation and Kūṭiyāṭṭam performance may help to reveal the cultural power of Sanskrit humour in early modern Kerala.
Diseño de un índice de salud estructural de puentes para priorización de intervenciones técnicas en Costa Rica
Authors: Ortiz-Quesada Giannina , Garita-Rodríguez César , Navarro-Mora Angel , Paez-González Gerardo , Alfaro-Acuña Alejandro 
Published: April 2024

Actualmente, Costa Rica no cuenta con una metodología rigurosa que considere las variables requeridas para calificar la condición de la salud estructural de un puente o estructura de paso. Algunas instituciones utilizan criterios diversos para asignar una calificación a la evaluación de una estructura y esto genera que se obtengan resultados diferentes al priorizar intervenciones. La priorización de los proyectos de infraestructura vial es clave para invertir los recursos de la forma más eficiente posible. En este contexto, este artículo presenta una propuesta que busca estandarizar un índice de la condición de la salud estructural de un puente, que permita priorizar y asignar recursos de forma objetiva. Para este índice se utiliza la evaluación de la condición estructural de elementos críticos, así como matrices de riesgo relacionadas con amenazas sísmicas e hidrometeorológicas. Como resultado, el diseño de este índice involucra el análisis de una gran cantidad de variables mediante criterios técnicos y estadísticos.
A decolonialidade no pensamento freiriano
Authors: Tomelin Nilton Bruno , Rausch Rita Buzzi , Muller Antônio José 
Published: April 2024

Este texto trata do conceito de Círculo de Cultura, trazido por Freire, como espaço crítico, criativo à uma educação libertadora e protagonista da relação entre a decolonialidade. Realizou-se uma pesquisa teórica para conhecer parte do pensamento freiriano e alguns elementos da decolonialidade. Freire (2000a, 2000b, 1999, 1996a, 1996b, 1985a, 1985b, 1970), Dussel (1993), Mignolo (2017), Quijano (2000) e Walsh (2012, 2005) permitem uma proximidade maior com a temática proposta. O objetivo do estudo é compreender as possíveis relações entre o pensamento de Freire e a decolonialidade na formação crítica, criativa e libertadora de educandas e educandos por meio do Círculo de Cultura. O estudo aponta para o protagonismo do Círculo de Cultura na relação entre a decolonialidade e o pensamento de Freire pautado na pedagogia dialógica, na prática reflexiva e de libertação. Por eles é possível mobilizar um processo insurgente para uma educação outra pautada na promoção da criticidade, criatividade e liberdade.
Pengaruh Kepemimpinan Transformasional terhadap Kepuasan Kerja dengan Pemberdayaan Psikologis sebagai Variabel Mediasi pada Guru SMK Swasta di Kota Bekasi
Authors: Muktia Reftina , Parimita Widya , Dalimunthe Sholatia 
Published: April 2024

ABSTRACT
A school is a formal institution established by the government to serve as a platform for the perpetuation of the educational system. This research aims to investigate the impact of transformational leadership on job satisfaction among Private Vocational School Teachers in Bekasi City, with psychological empowerment as a mediator. The study employed a descriptive survey methodology, utilizing a sample of 104 participants selected from a population of 189 individuals. The investigator opted to employ a path analysis framework to evaluate the conjecture. Based on the result, it can be inferred that the variables of transformational leadership exert an impact on job satisfaction, whereby psychological empowerment serves as a mediating variable that operates indirectly.
Keywords: transformational leadership, psychological empowerment, job satisfaction.

ABSTRAK
Sekolah adalah lembaga formal yang didirikan oleh pemerintah sebagai wadah untuk melanggengkan sistem pendidikan. Penelitian ini bertujuan untuk mengetahui pengaruh kepemimpinan transformasional terhadap kepuasan kerja pada Guru SMK Swasta di Kota Bekasi, dengan pemberdayaan psikologis sebagai mediatornya. Studi ini menggunakan metodologi survei deskriptif, menggunakan sampel 104 peserta yang dipilih dari populasi 189 orang. Penyidik ​​memilih untuk menggunakan kerangka kerja analisis jalur untuk mengevaluasi dugaan. Berdasarkan hasil penelitian disimpulkan bahwa variabel kepemimpinan transformasional berpengaruh terhadap kepuasan kerja, dimana pemberdayaan psikologis berfungsi sebagai variabel mediasi yang bekerja secara tidak langsung.
Kata Kunci: kepemimpinan transformasional, pemberdayaan psikologis, dan kepuasan kerja.
Repeated γ Irradiation and Thermal Annealing via Built-In Thermo-Electric Coolers of Si Avalanche Photodiodes
Authors: Wu Shuin Jian , Chowdhury Arya , Soe Moe Thar , Ling Alexander 
Published: April 2024

Abstract
When operating in space, on-board Silicon Geiger-Mode Avalanche Photodiodes (GM-APDs) are exposed to radiation damage that result in an increase in dark count rates. Thermal annealing has been found to mitigate the damage, although prior studies have largely focused on annealing following a single session of proton irradiation. This work reports that thermal annealing can be performed simply with the built-in thermo-electric coolers of the GM-APDs. Annealing was also done in 10 min intervals for a clearer view of the recovery curve. Mitigation of damage from repeated γ radiation was observed from the: (1) halving of the increase in dark count rates on average and (2) outperformance of room temperature annealing (25°C) by 33%. Additionally, we show that heavy doses of γ radiation (21 krad) have a probability of causing Random Telegraph Signals in GM-APDs that can be suppressed by lowering the operating temperature.
Transmission Network of Hepatitis C Virus Subtype 2a in Huazhou County, Shaanxi Province, China
Authors: Zhang Mengyan , Ning Shaoqi , Zhang Luqian , Liu Gang , Chen Sa , Zhang Yi 
Published: April 2024

Abstract

Background In Shaanxi Province, Huazhou County is one of the five highest rates of HCV infection incidence and prevalence. Understanding its characteristics of HCV transmission patterns could help guide targeted preventative strategies. This study employed phylogenetic analysis and the construction of a molecular transmission network of HCV-infected people in Huazhou County, to describe the predominant strains of HCV and identify factors associated with onward transmission.Methods The whole blood samples were obtained from HCV RNA positive individuals for NS5B sequencing. A maximum-likelihood phylogenetic tree was constructed to determine HCV subgenotypes. Bayesian phylogenetic analysis was employed to estimate the evolutionary history. The transmission network based on maximum-likelihood phylogenetic tree and pairwise distance was constructed. Logistic regression was used to identify the factors associated with clustering in the transmission network.Results 61 HCV NS5B sequences were successfully amplified and sequenced. The 61 sequences were found to all belong to subtype 2a by maximum-likelihood phylogenetic analysis. Bayesian phylogenetic analysis showed that the majority of the subtype 2a have descended from the northwest of China with estimated dates of 8 to 20 years before sampling. A transmission network was constructed with a clustering rate of 26.2%. Multivariate logistic regression showed individuals with a history of blood transfusion and living at Shi Village, Huazhou County were associated with clustered in the transmission network.Conclusion HCV transmission in Huazhou County was predominant by subtype 2a. High risk of transmission was associated with HCV infections in residential Shi Village, Huazhou County and people with a history of blood transfusions. Prioritizing targeted interventions for these groups may help to prevent infections.