bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Serratia marcescens MY1112 as Probiotic Bacteria for Tiger Shrimp Culture in the Acid-sulfate Soil Ponds
Authors: Atmomarsono M , Nurbaya  , Nurhidayah  , Susianingsih E 
Published: April 2024

Abstract
Probiotic bacteria have been commonly used for disease prevention in aquaculture systems, but sometimes these probiotic bacteria do not work in certain ponds. This experiment aimed to find out if the probiotic bacteria of Serratia marcescens MY1112 could work properly for tiger shrimp culture in acid-sulfate soil ponds. Eight 0.5-ha ponds located in Samataring village of Sinjai regency were used. Two treatments of bacteria probiotic combinations were applied here, namely A) Brevibacillus laterosporus BT951, Serratia marcescens MY1112, and Bacillus licheniformis BM58; and B) Use of Bacillus subtilis BM12 to replace S. marcescens MY1112 in the bacteria combination. The shrimp production and their survival rate in treatment A were significantly better than those of treatment B. However, probiotic bacteria of Serratia marcescens MY1112 could work better in the acid-sulfate soil pond if combined with the dolomite application weekly.
Habitat suitability model of future coral reef region in the Halmahera Waters under the projection of environmental pressures from future climate change and coastal pollution
Authors: Hidayah I , Januar H I , Humaida N 
Published: April 2024

Abstract
The coral reef region of the Halmahera waters situated within the tropical area of the coral triangle, covers approximately 193,126 km2, with coral reef habitats occupying roughly 958.47 km2. The coral reefs in Halmahera waters are essential for ecosystem services and local fisheries. However, they are at risk from climate change and coastal pollution. This study aimed to develop habitat suitability models for coral reefs in the Halmahera waters under future climate change scenarios. MaxEnt modeling was employed to project suitability for the coral Acropora spp. using occurrence data. Suitability projections were made for 2050 under IPCC RCP 2.6, 6.0, and 8.5 scenarios, assuming no measures are taken to mitigate coastal water pollution. Environmental factors considered in the modeling included temperature, salinity, current velocity, pH, sunlight penetration, and chlorophyll levels. Results demonstrated a significant decline in habitat suitability, with an 85% loss of highly suitable areas under RCP 2.6 – 8.5 compared to current conditions (741 km2). Chlorophyll levels, a marker of coastal pollution, were predicted to be the most influential factor, followed by temperature and salinity. The findings highlight the need to reduce global carbon emissions and implement conservation measures to mitigate coastal water pollution in preserving coral reef habitats.
The river health of Alista River based on macroinvertebrates communities at Selorejo Village Dau Subdistrict Malang Regency
Authors: Sudaryanti S , Herawati E Y 
Published: April 2024

Abstract
The Alista River is one of the rivers in the upstream Brantas watershed which has relatively natural conditions, but currently there are agricultural activities on the riverbank. The aim of this study was to analyze the health status in Alista River based on the macroinvertebrate communities. This study uses a survey method. Sampling was carried out at 5 stations based on different land use. Sampling of macroinvertebrates used the kicking technique with a hand net with a mesh size of 500 μm in a 10 meter long riffle area. Data analysis used the value of BMWP index (Biological Monitoring Working Party) and the ASPT (Average Score Per Taxon). The results of the identification of macroinvertebrates found were 38 families for instance Glossossomatidae, Lepidosmatidae, and Richardsonianidae. The families belong to 10 orders and 3 classes. The lowest family is 11 families at station 5 (citrus and bamboo gardens). The highest families are 29 families at station 3 (citrus gardens and forests). The results show BMWP-ASPT score ranged from 6.3–4.3 indicating excellent to moderately poor health status. Stations with excellent categories, namely stations 1,2,3,4. Station land use 1,2,3 secondary forest before tourism was discovered including Glossosomatidae. Station 4′s land use is in the form of a tourism location and Lumbriculidae has been found. Stations in the moderately poor category, namely station 5 which is located after tourism sites (citrus gardens and settlements) were found Richardsonianidae.
Distribution of microplastics in Lusi Island, Sidoarjo, Indonesia
Authors: Salamah L N , Al-Zamzami I M , Pramudia Z , Susanti Y A D , Dhea L A , Kurniawan A 
Published: April 2024

AbstractLusi Island, situated downstream of the Brantas River, is anticipated to harbour significant microplastics from Brantas River runoff. Consequently, a thorough analysis of the microplastic presence on Lusi Island is crucial for effective pollution mitigation. Despite the critical need for such investigations, more studies must report the abundance of microplastics in this locale. This study aims to fill this gap by examining the abundance of microplastics in the downstream area of the Brantas River, specifically Lusi Island. The research not only delves into the prevalence of microplastics but also investigates various water quality parameters, including dissolved oxygen, pH, temperature, turbidity, and total dissolved oxygen. The study reveals that the abundance of microplastics in Lusi Island ranges from 597 to 787 particles per litre in surface water samples. Three distinct types of microplastics—fragments, fibres, and film—were identified on the island. Notably, microplastic fragments dominate in number compared to other forms. Despite the favourable water quality parameters suggesting a healthy aquatic environment, the study underscores the necessity of implementing mitigation measures to address microplastic pollution effectively.
Blood and Micronuclei Analysis of Tawes Fish (Barbonymus gonionotus) Correlation with Water Quality in the Boon Pring Ecotourism Area, Turen, Malang Regency
Authors: Hertika A M S , Risjani Y , Buwono N R , Kei S A A , Heratiana Y Y , Nur Z K , Ashari F I A , Alfarisi M A , Halimah M F 
Published: April 2024

AbstractTawes (Barbonymus gonionotus) is a type of endemic fish that lives in the Boon Pring Reservoir, Turen, and Malang Regency. This study aims to analyze the water quality and blood profile of Tawes Fish (Barbonymus gonionotus) in Boon Pring Ecotourism and to determine the relationship between water quality and fish health through their blood profile using the Canonical Correspondence Analysis (CCA) method. The results obtained after measuring the water quality during two samplings at both locations were that the temperature ranged from 21.7–27C, TDS 111–145 mg/l, TSS 2-4 mg/l, pH 6-6.7, DO 8–10.1 mg/l, BOD 0.44-0.98 mg/l, and ammonia 0.026–0.075 mg/l. Both locations are classified as having good or normal conditions. Observations on the blood profile of Tawes fish (Barbonymus gonionotus) are healthy and normal. The average number of erythrocytes was 2,586,667 cells/mm3, leukocytes were 60,500 cells/mm3, lymphocytes were 76.8%, monocytes were 1.4%, neutrophils were 3.3%, hemoglobin was 9.8 g/mL, hematocrit was 34.6%, and micronuclei were 12.3 cells/1000. The results of data analysis using CCA showed that all blood and micronuclei profiles of fish were affected by water quality parameters, both significantly and moderately to severely.
Study of the structure and characterization of membranes reinforced with CuO-NPs/Graphene Oxide using bacterial cellulose extracted from Sargassum sp. for water nanofiltration system
Authors: Yanuhar U , Evanuarini H , Musa M , Suryanto H , Maulana J , Wardani N P , Machfuda D R , Caesar N R 
Published: April 2024

Abstract
Bacterial cellulose membranes find extensive applications in industries involving water purification, wastewater treatment, and biomedical uses. Nevertheless, prevailing membranes suffer from drawbacks like water flow hindrances and fouling susceptibility. Hence, the pressing need for more efficient and robust BC membranes. This study aims to assess the impact of a hybrid treatment involving Copper Oxide nanoparticles (CuO-NPs) and Graphene Oxide (GO) on bacterial nanocellulose membranes. The research employed two treatments: (1) control (BNCA), and (2) Bacterial cellulose infused with 0.5 wt% CuO-NPs/GO. The nanocellulose production involved a high-pressure homogenizer, followed by acetate nanocellulose synthesis and nanocomposite membrane functionalization with CuO nanoparticles. SEM, FTIR, and XRD analyses characterized the membranes. Successfully formed seaweed-derived bacterial cellulose had a thickness of 1-3 cm. Characterization showed it belonged to Cellulose type I with a crystalline degree ranging from 82.3% to 83.1%. FTIR analysis of dry BNCA membranes indicated changes in transmittance at 1738, 1554, and 764 cm-1 due to CuO-NPs/GO addition, altering the O-H bond in bacterial cellulose. Based on the results of the above research, it is evident that the Membrane Reinforced with CuO-NPs/Graphene Oxide has been successfully developed and holds potential as a water nanofiltration system.
The effect of liquid smoke (Cocos nucifera) in controlling acute hepatopancreatic necrosis disease in Litopenaeus vannamei
Authors: Nasuki  , Rizky Putri Nurhanida , Harijono T , Ritonga L B R , Edy M H , Suprakto B , Primasari K , Nazran  
Published: April 2024

Abstract

Vibrio parahaemolyticus is found in coastal environments throughout the world and is a major pathogen that causes Acute Hepatopancreatic Necrosis Disease (AHPND) with mass mortalities in Pacific whiteleg shrimp (Penaues vannamei). The extract of coconut shell liquid smoke (Cocos nucifera) was tested for antibacterial effect against AHPND in vitro. It was further evaluated for its potential efficacy in preventing AHPND in vannamei shrimp in vivo. The water in this study has been tested and positive for Vibrio parahaemolyticus. Two experimental diets were obtained by adding 0.5% and 1% liquid smoke to a commercial shrimp diet. The dietary supplementation with liquid smoke significantly enhanced shrimp growth performance during 90 days of rearing. A liquid concentration of 0.5% showed a higher growth performance (17.56 gr) than a 1% concentration (14.4 gr). Liquid smoke added to the shrimp fed partially entered the shrimp’s digestive system, and some would dissolve into the water. This water-soluble bioactive is thought to reduce the concentration of V. parahaemolyticus in waters. This was proven by the fact that no shrimp were infected with V. parahaemolyticus during the 90-day rearing.
Effect of various local natural ingredients fortified in fish feed on the gonad histology and water quality of tilapia (Oreochromis niloticus)
Authors: Hariati A M , Mahariawan I M D , Yuniarti A , Wiadnya D G R 
Published: April 2024

Abstract
The addition of natural ingredients fortified in fish feed was developed to enhance good formulation engineering. Currently, there is a need to improve follow-up research strategies for designed feed formulations using natural ingredients in tilapia farming. It is hoped that a sustainable and eco-friendly fish farming system will be improved. This study aimed to evaluate the effect of local natural ingredients fortified in fish feed on the gonad histology and water quality of tilapia (O. niloticus). Following these results, the histology test of tilapia showed that more than 50% of the gonad tended to be male in all treatments. This showed that the use of natural ingredients in the appropriate dosage in feed would affect the masculinization of tilapia. Furthermore, the specific growth rate (SGR) with the addition of natural ingredients to the feed showed results comparable to that of synthetic hormones. It can be concluded that the use of natural ingredients such as honey, cow testes, and Tribulus fortified in fish feed revealed a positive effect on the masculinization of tilapia and water quality levels in the fish farming system.
Pulsed electromagnetic field-assisted reduced graphene oxide composite 3D printed nerve scaffold promotes sciatic nerve regeneration in rats
Authors: Wang Zichao , Li Shijun , Wu Zongxi , Kang Yifan , Xie Shang , Cai Zhigang , Shan Xiaofeng , Li Qing 
Published: April 2024

Abstract
Peripheral nerve injuries can lead to sensory or motor deficits that have a serious impact on a patient’s mental health and quality of life. Nevertheless, it remains a major clinical challenge to develop functional nerve conduits as an alternative to autologous grafts. We applied reduced graphene oxide (rGO) as a bioactive conductive material to impart electrophysiological properties to a 3D printed scaffold and the application of a pulsed magnetic field to excite the formation of microcurrents and induce nerve regeneration. In vitro studies showed that the nerve scaffold and the pulsed magnetic field made no effect on cell survival, increased S-100β protein expression, enhanced cell adhesion, and increased the expression level of nerve regeneration-related mRNAs. In vivo experiments suggested that the protocol was effective in promoting nerve regeneration, resulting in functional recovery of sciatic nerves in rats, when they were damaged close to that of the autologous nerve graft, and increased expression of S-100β, NF200, and GAP43. These results indicate that rGO composite nerve scaffolds combined with pulsed magnetic field stimulation have great potential for peripheral nerve rehabilitation.
Silver nitrate-assisted photo-crosslinking for enhancing the mechanical properties of an alginate/silk fibroin-based 3D scaffold
Authors: Moon Seo Hyung , Choi Geunho , Cha Hyung Joon , Yang Yun Jung 
Published: April 2024

Abstract
Scaffolds play a pivotal role in tissue engineering and serve as vital biological substitutes, providing structural support for cell adhesion and subsequent tissue development. An ideal scaffold must possess mechanical properties suitable for tissue function and exhibit biodegradability. Although synthetic polymer scaffolds offer high rigidity and elasticity owing to their reactive side groups, which facilitate tailored mechanical and rheological properties, they may lack biological cues and cause persistent side effects during degradation. To address these challenges, natural polymers have garnered attention owing to their inherent bioactivity and biocompatibility. However, natural polymers such as silk fibroin (SF) and tyramine-modified alginate (AT) have limitations, including uncontrolled mechanical properties and weak structural integrity. In this study, we developed a blend of SF and AT as a printable biomaterial for extrusion-based 3D printing. Using photocrosslinkable SF/AT inks facilitated the fabrication of complex scaffolds with high printability, thereby enhancing their structural stability. The incorporation of silver nitrate facilitated the tunability of mechanical and rheological behaviors. SF/AT scaffolds with varying stiffness in the physiologically relevant range for soft tissues (51–246 kPa) exhibited excellent biocompatibility, indicating their promising potential for diverse applications in tissue engineering.
A study on the optimization and functionalization of zirconia-coated multi-walled carbon nanotubes through hydrothermal process
Authors: K Suhas , BRN Murthy , Hiremath Anupama 
Published: April 2024

Abstract
Carbon nanotubes (CNTs) have emerged as revolutionary nanomaterials with exceptional properties. This study focuses on optimizing acid treatment conditions for functionalizing multi-walled carbon nanotubes (MWCNTs) and investigates the synthesis of zirconia coated MWCNTs. Acid treatment was conducted with HNO3 and H2SO4 their combinations with different molarity concentrations. Acid oxidation, particularly with nitric acid, enhances CNT solubility and reactivity. The research explores the incorporation of acid treated MWCNTs into ceramic composites, addressing challenges in dispersion and bonding. Additionally, a hydrothermal process is employed to coat MWCNTs with zirconia, contributing to the development of nanocomposites with unique properties. Characterization studies, including SEM, TEM, XRD, and Raman spectroscopy, provide insights into structural and chemical aspects. The findings contribute to advancing the application of CNT-based nanocomposites in various technological fields. The study’s methodology, involving acid treatment optimization and hydrothermal synthesis, provides a robust foundation for further advancements in the field of nanomaterials and composites. This novel approach results in ZrO2-coated MWCNTs, showcasing potential applications in catalysts, oxygen sensors, fuel cells, electronic devices and also as a reinforcing material.
Solvent free fabrication and thermal tuning of copper oxide-zirconium dioxide nanocomposite for enhanced photocatalytic efficacy
Authors: Zain Maqsoom , Yasin Khawaja Ansar , Haq Sirajul , Shujaat Shafia , Syed Asad , Elgorban Abdallah M , Bahkali Ali H , Razzokov Jamoliddin , Rehman Zia Ur 
Published: April 2024

Abstract
Recently, several methods has been used for the synthesis of bimetal oxide nanocomposite, however, very few studies are available on the solvent free mechanochemical synthesis of nanomaterials. In this study, mortar and pestle assisted fabrication of copper oxide-zirconium dioxide nanocomposite (CuO–ZrO2 NC) was carried out and was calcined at 300, 600 and 900 °C. The variation in crystallographic parameters was examined through x-ray diffraction (XRD) and the crystallite size was found to be gradually increased with increasing calcination temperature. The morphological changes with increasing calcination temperature were traced during scanning electron microscopy (SEM) analysis. The percentage elemental composition was verified through energy dispersive x-ray (EDX) spectroscopy whereas the functional group analysis was done through Fourier transform infrared (FTIR) spectroscopy, where the intensity of peaks assigned to hydroxyl moiety decreased with increasing calcination temperature. The CuO–ZrO2 NCs were used as a photocatalysts for the degradation of the Fluorescein in the presence of solar light and highest photodegradation (77.27%) was noticed for the CuO–ZrO2 NC calcined at 900 °C.
An optically pumped magnetic gradiometer for the detection of human biomagnetism
Authors: Cook Harry , Bezsudnova Yulia , Koponen Lari M , Jensen Ole , Barontini Giovanni , Kowalczyk Anna U 
Published: April 2024

Abstract
We realise an intrinsic optically pumped magnetic gradiometer based on non-linear magneto-optical rotation. We show that our sensor can reach a gradiometric sensitivity of 18 fT

cm

−
1

Hz

−
1

and can reject common mode homogeneous magnetic field noise with up to 30 dB attenuation. We demonstrate that our magnetic field gradiometer is sufficiently sensitive and resilient to be employed in biomagnetic applications. In particular, we are able to record the auditory evoked response of the human brain, and to perform real-time magnetocardiography in the presence of external magnetic field disturbances. Our gradiometer provides complementary capabilities in human biomagnetic sensing to optically pumped magnetometers, and opens new avenues in the detection of human biomagnetism.
Recyclable plastic waste segregation with deep learning based hand-eye coordination
Authors: Sundaralingam Sathiyapoobalan , Ramanathan Neela 
Published: April 2024

Abstract
Plastic waste management is a critical concern in municipal solid waste management systems worldwide. Despite the efforts of waste management personnel to segregate waste manually, the existing challenges persist. In municipal waste facilities, individuals responsible for waste segregation face numerous obstacles. Consequently, a significant amount of plastic waste ends up in landfills, exacerbating the plastic waste problem. To overcome these challenges, this research focuses on developing an automated system capable of categorizing plastic waste based on its visual characteristics. The trained model exhibits high precision in identifying various types of plastic waste, including PET, HDPE, PVC, LDPE, PP, and PS. Specifically, the model achieves an Average Precision of 0.917 and an Average Recall of 0.801. Moreover, the model maintains a good balance between precision and recall. In real-time operation, an overhead camera locates the positions of both the waste items and the gripper. By calculating the positional difference between the waste and the gripper, the system achieves a higher level of segregation accuracy, resembling human-like hand-eye coordination. The proposed system offers a solution to the challenges faced in MSW facilities, where the timely segregation of waste is crucial. By automating the plastic waste categorization process, the system can significantly improve waste management practices, leading to a more sustainable approach to plastic waste disposal and recycling.
Analysis of mechanical and shape memory properties of biocompatible shape memory polymers with different Fe3O4 contents
Authors: Kim Minseok , Kim Young Bin , Lee Minjong , Chun Heoung-Jae 
Published: April 2024

Abstract
Shape memory polymers (SMPs) can recall their original shape when exposed to environmental factors like heat, light, or magnetic fields. These SMPs are extensively used in medical applications, notably in stents, due to their shape memory and biocompatibility after implantation in the human body. However, conventional stents require balloons for expansion, limiting their flexibility. To address this, the study developed an SMP material that can regain its original shape without the need for a balloon. To effectively induce self-response, magnetically responsive Fe3O4 nanoparticles at concentrations of 10%, 15%, and 20% were integrated, and their dispersion was verified using scanning electron microscopy. The study also utilized differential scanning calorimetry to measure the glass transition temperature, crystallization temperature, and melting point of the synthesized polymers. The confirmation of uniformly dispersed nanoparticles revealed that the synthesized polymers exhibited a high shape memory ratio (84%–93%). Shape memory ratio represents the ability to return from a temporarily deformed state to the initial, original shape. Therefore, the biocompatible, magnetic-responsive SMP developed in this study has the potential to be utilized in various medical devices as an advanced shape-memory material.
Optimizing surface roughness of workpieces made of stainless steel 316L with DMLS technology: a Taguchi method approach
Authors: Jakupi Kaltrine , Dukovski Vladimir , Abdullahu Fitore 
Published: April 2024

Abstract
This study employs Direct Metal Laser Sintering (DMLS) technology to investigate surface roughness in stainless steel 316L 3D printing processes. Utilizing the Taguchi method in experimental design, we examine the influence of independent variables—laser power, scan speed, and hatch spacing - on surface roughness quality. Results indicate that laser power has the greatest impact, followed by scan speed and hatch spacing. Notably, both laser power and hatch spacing positively affect surface roughness, while scan speed adversely affects the top surface quality of printed components. This research enhances comprehension of the intricate relationship between process parameters and surface quality in DMLS-based 3D printing, offering insights for optimizing surface roughness in stainless steel 316L applications. The study holds practical significance for enhancing the quality and performance of 3D-printed components across diverse engineering and manufacturing sectors.