bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diffuse interface model for fluid lipid vesicles with area-difference elasticity
Authors: Bottacchiari Matteo , Gallo Mirko , Bussoletti Marco , Casciola Carlo Massimo 
Published: February 2025

Abstract
Lipid bilayer membranes are fundamental biological barriers both at the cellular and sub-cellular level. They are both very stable and extremely deformable, characteristics that make membrane vesicles an efficient system for drug delivery applications. In most cases, due to the scale separation between the membrane thickness and the vesicle size, fluid lipid vesicles can be described as elastic sheets that deform as prescribed by a curvature dependent energy. At the same time, vesicle scale and membrane thickness may become simultaneously important in several key biological processes, such as vesicle fusion/fission, which are also pivotal steps for drug delivery. Recently, we provided a diffuse interface description of lipid vesicles that contains both the large scale of the vesicle and the small thickness of the membrane, allowing to account for multiscale effects in membrane fusion/fission (Bottacchiari et al. in PNAS Nexus 3:300, 2024). Here, after reviewing the main features of the approach and the related results, we analyze an additional term for the diffuse interface that takes into account the so-called area-difference elasticity, namely an energy term that considers the cost for the flip-flop motion of a lipid between the two monolayers constituting the bilayer membrane. Results are validated against those obtained with the (sharp-interface) area-difference elasticity model.
An Experimental Method for Fatigue Testing Cast Iron Water Pipes Using Combined Internal Water Pressure and Bending Loads
Authors: John E. D. A. , Boxall J. B. , Collins R. P. , Bowman E. T. , Susmel L. 
Published: February 2025

Abstract

Background
Investigations into the fatigue failures mechanism of Grey Cast Iron (GCI) water pipes are inhibited by the lack of a lab-based method to conduct extensive high-cycle biaxial fatigue test programmes.

Objective
The work presented in this paper developed and tested a novel experiment capable of causing controlled fatigue failures of GCI pipe specimens in the high-cycle fatigue regime using bending and internal water pressure fatigue loading.

Methods
A novel four-point bending and internal water pressure fatigue testing system was developed to apply constant amplitude out-of-phase biaxial loading to 58 mm diameter GCI pipes at 1.7 Hz. To verify the ability of this equipment to apply known stresses and repeatable loads to pipe specimens a series of tests were conducted. A finite element model of the pipe specimen was used to estimate the strains and displacements applied by the equipment.

Results
Experimental strains and displacements were mainly within ± 10% of the estimated values and the pressure amplitudes measured over 103 cycles were within ± 3% of the average. Dynamic load effects occurred at higher bending loads, but these were quantified and accounted for. Trial destructive tests revealed that the lifespan of leaking fatigue cracks in GCI pipes with uniform wall-loss subject to combined internal pressure and bending fatigue loads is less than 1% of the total cycles-to-burst.

Conclusions
The experimental method developed was able to apply combined, out-of-phase internal pressure and bending fatigue loads accurately and consistently to small-dimeter GCI pipes, and cause these pipes to develop high-cycle fatigue regime failures.
Impact of Mlkl or Ripk3 deletion on age-associated liver inflammation, metabolic health, and lifespan
Authors: Mohammed Sabira , Ohene-Marfo Phoebe , Jiang Chao , Peng Zongkai , Thadathil Nidheesh , Tran Albert , Nicklas Evan , Bhaskaran Shylesh , Wang Dawei , Selvarani Ramasamy , Singh Amit , Yang Zhibo , Ahsan Nagib , Deepa Sathyaseelan S. 
Published: February 2025

Abstract
Chronic, low-grade inflammation is a hallmark of aging and various age-related diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD). The prevalence of metabolic dysfunction-associated steatohepatitis (MASH), an advanced form of MASLD, increases with age and contributes to morbidity and mortality among the elderly. This study investigates the role of necroptosis, a programmed cell death pathway that promotes inflammation, in liver inflammaging and age-associated MASLD by utilizing genetic ablation models of two key necroptosis proteins, Mlkl or Ripk3. The absence of Mlkl or Ripk3 significantly reduced liver inflammation, steatosis, and fibrosis in aged male mice, supporting the role of necroptosis in age-associated MASLD. Additionally, Mlkl or Ripk3 deletion impacted other non-necroptotic cellular processes that drive inflammation and MASLD, such as cellular senescence, apoptosis, and autophagy in aged liver. Levels of plasma TNFα and IL6, key proinflammatory cytokines associated with inflammaging, are reduced in Mlkl

−/−
or Ripk3

−/−
aged mice, supporting a systemic effect of necroptosis inhibition on inflammation. Proteomic analysis of liver tissues emphasizes the critical role of lipid and immune regulatory processes in maintaining liver homeostasis when Mlkl or Ripk3 is absent in aging liver. While Mlkl deletion did not affect the lifespan of mice, Ripk3 deletion shortened it. Additionally, Mlkl deficiency improved insulin sensitivity, whereas Ripk3 deficiency exacerbated glucose intolerance in aged mice. Thus, selective inhibition of Mlkl, not Ripk3, represents a potential therapeutic avenue for mitigating age-related liver disease and enhancing metabolic outcomes in the elderly.
Association of Primary Care Access with Health-Related ChatGPT Use: A National Cross-Sectional Survey
Authors: Ayo-Ajibola Oluwatobiloba , Julien Catherine , Lin Matthew E. , Riddell Jeffrey , Duan Naihua , Kravitz Richard L. 
Published: February 2025

Abstract

Background
ChatGPT has quickly gained popularity as a source of online health information (OHI). However, it is unclear how having a usual source of primary care (USPC) is related to OHI-seeking.

Objective
Explore how having a USPC and other characteristics thought to affect access-to-care influence the use of ChatGPT and other OHI forms.

Design
Cross-sectional national survey.

Participants
Adult members of ResearchMatch, a non-profit affiliate of the National Institutes of Health, between June and August 2023.

Main Measures
The survey evaluated demographics, health characteristics, and OHI-seeking behaviors, including ChatGPT usage. OHI sources were categorized as “passive” (Google, Wikipedia, WebMD) and “interactive” (forums, Q&A sites, ChatGPT). Descriptive statistics, t-tests, and chi-square tests compared users by USPC status. Multiple logistic regression estimated adjusted effects on ChatGPT use.

Key Results
Of 21,499 adults invited to participate in the survey, 2406 (11.2%) responded. Among respondents, 56% reported having a USPC. Those with a USPC, compared to those without, were older, spoke English as their primary language, had higher income, and had more formal education (all p<.001). Participants with a USPC were more likely to use passive OHI (OR 2.46, 95% CI 1.55–3.90, p<.001) and less likely to use interactive OHI (OR 0.73, 95% CI 0.60–0.89, p=.002) or ChatGPT (OR 0.56, 95% CI 0.44–0.71, p<.001). Age over 50 (OR 0.11, 95% CI 0.06–0.20, p<.001), non-White race (OR 0.51, 95% CI 0.38–0.70, p<.001), very good or better health (OR 0.71, 95% CI 0.55–0.92, p=.009), and college education (OR 0.61, 95% CI 0.39–0.97, p=.035) were inversely related to ChatGPT use.

Conclusions
In this national survey of patients participating in a clinical research matching service, those with regular primary care access relied less on ChatGPT, suggesting that a personal primary care relationship may attenuate the need or motivation to use AI-derived OHI.
Adoption of Hydrogen as a Climate-Neutral Reducing Agent for Recovering Metals from Copper Slag
Authors: Chen Min , Sukhomlinov Dmitry , Taskinen Pekka , Hamuyuni Joseph , Tiljander Mia , Lindgren Mari , Lindberg Daniel 
Published: February 2025

Abstract
This study explores a sustainable approach for the recovery of metal values from copper slag using hydrogen (H2). The high-temperature isothermal reduction experiments were carried out under a controlled H2-Ar atmosphere and the subsequent molten slag extraction technique was employed. The elemental concentrations in both the metal/matte and the reduced slag were examined using inductively coupled plasma optical emission spectroscopy and electron probe microanalysis. It was found that the submerged H2 blowing technique has a superior impact to top surface blowing on recovering metals from copper slag due to improved mass and heat transfer in the slag. Approximately 65 pct of copper of the initial 1.19 wt pct can be reduced into copper alloy after reduction by submerged H2 blowing with the lowest concentration of 0.4 wt pct in slag. More than 90 pct of zinc and lead deported into the gas phase as metals. This research contributes to the ongoing efforts to enhance the sustainability of metallurgical processes and reduce their environmental footprint.

Graphical Abstract
New Insights Into Hydrogen Reduction of Hematite in an Indirectly Heated Flash Reactor from Measurements and First-Order Modeling
Authors: Mokhtarani Babak , Jafarian Mehdi , Sabah Shabnam , Dufty Tom , Brooks Geoffrey , Saw Woei , Chinnici Alfonso , Nathan Graham J. 
Published: February 2025

Abstract
New pilot-scale experimental data are reported for hematite reduction with hydrogen in an indirectly heated flash reactor, named the Zero Emission Steel Technology (ZESTY) reactor, together with analysis with a one-dimensional well-stirred reactor and kinetic model. The process feeds particles by gravity through a tubular reactor, which is indirectly heated through the walls that are maintained at a range of uniform temperatures from 850 to 1100 °C to achieve metallization of up to 95 pct. Process modeling is performed with Aspen Plus utilizing two kinetic models, namely the modified Sohn and mixed control models, together with a number of assumptions. The kinetic parameters are then adjusted with experimental data to best match the measured conversion. Calculations for hematite particle residence times consider particle velocity within the reactor, alongside particle size with the density modified to account for conversion extent. The modified model provides a reasonable match of the increase in the metallization degree of hematite with rising temperatures across the range of operating conditions. Moreover, elevating the hydrogen stoichiometric ratio correlates with increased metallization degree. Also as expected, an increase in the particle size adversely influences the metallization degree through decreasing the residence time. The level of agreement and internal consistency increases confidence in the reported results and interpretation of the measurements.
USP14 targets FABP5-mediated ferroptosis to promote proliferation and cisplatin resistance of HNSCC
Authors: Qian Jiaxin , Zhao Zitong , Ma Liying , Liu Wensheng , Song Yongmei 
Published: February 2025

Abstract

Background
Head and neck squamous cell carcinoma (HNSCC) ranks among the most lethal solid tumors in humans, with a five-year survival rate hovering around 50%. The limited understanding of its biological foundation has hindered the development of efficacious targeted therapeutics.

Methods
TCGA database and immunohistochemistry were deployed to confirm the expression levels of ubiquitin specific protease 14 (USP14). CCK8 method was used to evaluate the influence of USP14 on cisplatin resistance. Further investigations into the role of USP14 were conducted through assessments of cell proliferation, colony formation, and Transwell assays. The impact of USP14 expression on ferroptosis was evaluated by measuring GSH/GSSG ratios, Fe2+ concentrations, and lipid peroxide levels. Co-IP was employed to verify the interaction between USP14 and FABP5.

Results
Our analysis revealed that USP14 ranked among the most prominently upregulated deubiquitinases (DUBs) in tissue samples of HNSCC. Notably, aberrant USP14 expression was linked to tumorigenesis and the malignant evolution of HNSCC and further suggested a poor prognosis. In vitro experiment revealed that USP14 depletion markedly inhibited cell growth, cisplatin resistance, invasion and migration capabilities of HNSCC cells. Mechanically, USP14 inhibits FABP5 ubiquitination and degradation, thus positively modulating FABP5 expression. Subsequent analyses demonstrated that the loss of USP14 promoted ferroptosis in HNSCC cells. Finally, in vivo xenograft experiments confirmed that the USP14 small molecular antagonist IU1 could effectively attenuate cisplatin resistance in HNSCC.

Conclusion
The results indicate that the USP14-FABP5 axis exerts oncogenic effects on HNSCC, providing a potential target for diagnosing and treating this type of malignancy.
Epidemiology of colorectal cancer: A review with special emphasis on India
Authors: Shivshankar Samyukta , Patil Prachi S. , Deodhar Kedar , Budukh Atul M. 
Published: February 2025

Abstract
Colorectal cancer (CRC) is a common malignancy and cause for death around the world. In India, it ranks as the fourth most incident cancer in both sexes, with 64,863 cases and 38,367 deaths in 2022. With such high mortality, CRC survival in India is way lesser than that of developed countries. While western countries are facing an overall decline in CRC incidence, various regions in India are seeing an increasing trend. Within India, urban regions have markedly higher incidence than rural. Risk factors include consumption of red and processed meat, fried and sugary food, smoking and alcohol, comorbidities such as obesity, diabetes and inflammatory bowel disease (IBD), family history of CRC, adenomas and genetic syndromes, radiation exposure, pesticides and asbestos. Consumption of nutrient-rich well-balanced diets abundant in vegetables, dairy products, whole grains, nuts and legumes combined with physical activity are protective against CRC. Besides these, metformin, aspirin and micronutrient supplements were inversely associated with the development of CRC. Since a considerable proportion of CRC burden is attributed to modifiable risk factors, execution of population level preventive strategies is essential to limit the growing burden of CRC. Identifying the necessity, in this review, we explore opportunities for primary prevention and for identifying high-risk populations of CRC to control its burden in the near future.
Where do maverick organization design ideas come from? Approaches to studying the development of organizational design knowledge
Authors: Heusinkveld Stefan , Smits Armand 
Published: February 2025

Abstract
This paper develops a framework comprising a typology of distinct approaches to understanding the development of maverick organization design ideas: design science, life-cycle, dramaturgical and translation. These approaches represent influential but distinct research traditions that vary substantially in their primary assumption of envisioned outcomes and drivers of design knowledge development. Yet, in combination, the insights from these approaches extend research into design theory, as well as provide a broader and more integrative basis for management practitioners to adequately dealing with new maverick organization design ideas.
Claims to universal norms and the forging of transnational networks: Struggles over the meaning of gender-based and sexual violence in conflicts
Authors: Zöhrer Michaela , Methfessel Christian , Stahl Daniel 
Published: February 2025

Abstract
The article analyzes the ways in which claims to universal norms promote, or complicate, collaboration in the context of transnational activism. It emphasizes the potential of a constructivist understanding of universality, i.e. not as something intrinsic to certain norms, but rather as an empirically powerful assumption and a strategic tool. The argumentation is based on two case studies. The first looks at how networks of transnational solidarity were formed and conflicts between activist groups emerged in response to conflict-related sexual violence committed during the Bosnian War. The second example looks at responses to the crimes committed by ISIS in Iraq in 2014 and beyond, demonstrating how shifts within the field of transitional justice, and in particular the emergence of norms centered on the concerns and participation of survivors, have shaped the building of networks around the right to reparation.
Prognostic factors and treatment outcomes of allogeneic stem cell transplantation in lymphoid malignancy
Authors: Kim Hyungsoon , Chung Haerim , Kook Hye Won , Kim Soo-Jeong , Kim Yu Ri , Cho Hyunsoo , Cheong June-Won 
Published: February 2025

Abstract
Allogeneic stem cell transplantation (allo-SCT) is a salvage treatment option for patients with relapsed or refractory lymphoid malignancies. However, the clinical variables impacting outcomes in these patients remain unclear. We analyzed 58 patients who underwent allo-SCT for lymphoid malignancies, including B-cell lymphoma (BCL, n = 20), Hodgkin’s disease (n = 3), multiple myeloma (n = 9), natural killer/T-cell lymphoma (NK/TCL, n = 4), and TCL (n = 22). The median progression-free survival (PFS) was 27.4 months, while the median overall survival (OS) was 30.6 months. In univariate analysis, human leukocyte antigen (HLA) matching and complete remission status post-transplantation were associated with improved PFS and OS. However, only post-transplant response remained significant for both survival outcomes in the multivariate analysis. Moreover, HLA matching was associated with a significantly improved PFS in patients with BCL and NK/TCL, but with better OS only in those with BCL. Complete remission after transplantation was associated with better PFS and OS in patients with BCL, NK/TCL, and TCL. Our results indicate that post-transplant response is an important prognostic indicator in allo-SCT for lymphoid malignancies and may guide clinical decisions and additional treatment.
Two new marine species of Rhinebothrium (Cestoda: Rhinebothriidea) from stingrays from the Persian Gulf and Gulf of Oman
Authors: Omrani S. , Golzarianpour K. , Malek M. , Golestaninasab M. , Seiedy Marjan 
Published: February 2025

Abstract
The genus Rhinebothrium (Cestoda: Rhinebothriidea) comprises tapeworm species parasitizing elasmobranch hosts, particularly batoids. Despite numerous recent findings regarding the ecological importance of marine fish parasites throughout the world, the biodiversity of cestodes inhabiting fishes of the Persian Gulf and the Gulf of Oman remains understudied. Here, two new species of Rhinebothrium from stingrays from the Persian Gulf and Gulf of Oman are described: Rhinebothrium gossi sp. nov. and Rhinebothrium palmeri sp. nov. from Maculabatis arabica and Maculabatis randalli, respectively. However, each new cestode species is found with a lower frequency in the other host species, too. These new species were already subjected to a molecular analysis and the revealed genetic distinctiveness requires detailed morphological examinations at the species level. A combination of morphomeristic characteristics including body size, scolex features, proglottid morphology, and reproductive structures distinguish the new species from the other congeners. Although these new species are morphologically similar, however, they differ from each other in the number of testes (6–8 and 8–14), and bothridial loculi (50 and 42 in R. gossi sp. nov. and R. palmeri sp. nov., respectively). These findings contribute to our understanding of marine cestode diversity and underscore the importance of further research in this ecologically significant region.
Echinococcus granulosus’ laminated layer immunomodulates nitric oxide, cytokines, and MMPs in PBMC from rheumatoid arthritis patients
Authors: Ameur F. , Amri M. , Djebbara S. , Soufli I. , Boussa R.-S. , Benazzouz S. , Boutemine I.-M. , Benkhelifa S. , Bouchemal M. , Mekhloufi-Dahou C. , Hanni F. , Yakoubi M. , Lefkir S.T. , Abdellaoui S. , Arroul-Lammali A. , Idris N.S. , Belguendouz H. , Touil-Boukoffa C. 
Published: February 2025

Abstract
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that affects the joints. Treatments are symptomatic and can induce side effects in some patients. In this sense and based on previous studies, our aim was to investigate the ex vivo immunoregulatory effect of the laminated layer (LL) during rheumatoid arthritis. LL is the outside layer of parasitic cyst of the helminth Echinococcus granulosus.
Our main objective was to study the effect of LL on nitric oxide (NO) and cytokines production, matrix metalloproteinases (MMPs) activities, inducible NO synthase (iNOS) and nuclear factor κappa B (NF-κB) expression. In this context, cultures of peripheral blood mononuclear cells (PBMC) from Algerian RA patients in active (ARA) and inactive (IRA) stage of the disease were stimulated with LL extract (50, 100, 150μg/mL). However, PBMC from ARA patients were stimulated with methotrexate (MTX; 0.5μg/mL) and biological disease modifying anti-rheumatic drugs (bDMARDs): anti-TNFα (10μg/mL), anti-IL6 (10μg/mL), anti-CD20 (10μg/mL), alone or combined with LL (50μg/mL).
Our results showed that LL reduced NO, TNF-α, and IL-17A production, MMP9/2 activities, and iNOS/NF-κB expression in PBMC from ARA patients. Concomitantly, LL increases IL-10 and TGF-β1 production in the same cultures. Interestingly, the decrease in NO production induced by bDMARDs was greater in association with LL.
Collectively, our findings indicate a strong immunoregulatory effect of LL on NO, MMPs, and cytokines. LL probably acts through the NF-κB pathway. The development of biodrugs derived from LL of E. granulosus could be a potential candidate to modulate inflammation during RA.
Supplemental description of Stephanoprora ornata Odhner, 1902 (Digenea: Echinochasmidae) infecting the Nile crocodile, Crocodylus niloticus (Crocodylidae) from Namibia with emendation of Mesorchis Dietz, 1909 and a phylogenetic analysis
Authors: Cajiao-Mora K. , Dutton H.R. , Jacobs F.J. , Beytell P.C. , Netherlands E.C. , DuPreez L.H. , Bullard S.A. 
Published: February 2025

Abstract
The synonymies of the echinochasmid genera Mesorchis Dietz, 1909 and Monilifer Dietz, 1909 with Stephanoprora Odhner, 1902 remain contentious and unresolved with morphology. To explore the matter, we herein provide a supplemental description of the type species of Stephanoprora, Stephanoprora ornata Odhner, 1902, based on specimens we collected from the intestine of a Nile crocodile Crocodylus niloticus Laurenti, 1768 (Crocodylia: Crocodylidae) captured in the Kavango River, Namibia. No nucleotide information was available previously for S. ornata. Morphology plus 28S and ITS2 phylogenetic analyses suggested Stephanoprora is a monotypic genus that can be differentiated from other genera by having 26 collar spines. Stephanoprora differs from Mesorchis by the number and distribution of collar spines (26 [2 dorsal spines, 12 lateral spines, 12 corner spines] vs. 22 [2 dorsal spines, 12 lateral spines, 8 corner spines] in Mesorchis), length of the pre-pharyngeal oesophagus, pharynx position posterior to collar (vs. pre-pharyngeal oesophagus short, pharynx anterior to or at level of corner spines), and testes shape (elongate-ovoid, irregular in outline vs. ovoid, atypically elongated). Our 28S and ITS2 phylogenies recovered the new sequence of S. ornata (having 26 collar spines) sister to all remaining echinochasmid sequences, representing species that have 20–24 collar spines. Echinochasmus Dietz, 1909 and Mesorchis were recovered as paraphyletic. We retain Monilifer as a junior subjective synonym of Echinochasmus based a suite of morphological features related to body shape and genitalia and because the designated type for Monilifer was reassigned to Echinochasmus. We accept 25 species of Mesorchis.