bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of exogenous GA, IAA, ABA and ethylene on pear fruit during different development stages
Authors: Guo Zhi-Hua , Liu Hong , Zheng Si-Qi , Wang Xue-Ping , Xie Zhi-Hua , Zhang Zheng-Mao , Liu Jing-Rong , Li Ji-Mei , Lin Shao-Yan , Gu Chao , zhang shaoling 
Published: February 2024

Abstract
Phytohormones are very important for fruit development and ripening. However, it is unclear the role of phytohormones in pear fruit quality. In this study, gibberellin (GA), Indole-3-acetic acid (IAA), abscisic acid (ABA), and ethephon were selected to treat pear fruit at different stages,including 30, 45, 60, 75, and 90 days after flowering blooming (DAFB). As a result, exogenous GA treatment could promote fruit enlargement, decrease fruit firmness, and inhibit the accumulation of organ acids in ripening fruit (110DAFB). Exogenous IAA or ABA treatment could also promote fruit enlargement. Exogenous ABA or ethephon could promote the accumulation of soluble solids in ripening fruit (110DAFB), indicating the involvement of ABA and ethylene in fruit ripening. Quantitative real-time PCR (qPT-PCR) analysis suggested that PbZEP1, PbNCED.B, PbSDR4, and PbAO3 are the crucial genes for ABA biosynthesis, and PbACS1b and PbACO1 are the crucial genes for ethylene biosynthesis in pear fruit. EMSA and dual-luciferase assay suggested that PbABF.B and PbABF.C.2 directly bind to the PbACS1b promoter to enhance the activity, while PbABF.E.1 and PbABF.E.2 directly bind to the PbACO1promoter to enhance the activity. This result indicates that the four ABF proteins may be involved in ethylene biosynthesis during fruit ripening. Our study provides a foundation for the roles of GA, IAA, ABA and ethylene in pear fruit and reveals the ABA–ethylene cross-talking during fruit ripening.
Temporal Dynamics of User Activities: Deep Learning Strategies and Mathematical Modeling for Long-Term and Short-Term Profiling
Authors: Azzam Fatima , Ali Abdelmgeid , Kayed Mohammed , Ali Hussein 
Published: February 2024

Abstract
Profiling social media users is an analytical approach to generate an extensive blueprint of user’s personal characteristics, which can be useful for a diverse range of applications, such as targeted marketing and personalized recommendations. Although social user profiling has gained substantial attention in recent years, effectively constructing a collaborative model that could describe long and short-term profiles is still challenging. In this paper, we will discuss the profiling problem from two perspectives; how to mathematically model and track user’s behavior over short and long periods and how to enhance the classification of user’s activities. Using mathematical equations, our model can define periods in which the user's interests abruptly changed. A dataset consisting of 30,000 tweets was built and manually annotated into 10 topic categories. Bi-LSTM and GRU models are applied to classify the user’s activities representing his interests, which then are utilized to create and model the dynamic profile. In addition, the effect of word embedding techniques and pre-trained classification models on the accuracy of the classification process is explored in this research.
Patients with Parkinson’s Disease Seem to Have Preventive Effect for Osteoporotic Fractures with the Use of Bisphosphonate: A Nationwide Cohort Study
Authors: Jang Min Uk , Kim Min Seong , Kwon Young-Min , Hwang Jihyun , Subramanian Sivakumar Allur , Kim SeongHyeon , Kim Sung Jae , Cho Sang Won , Sung Il-Hoon 
Published: February 2024

Abstract
Patients with Parkinson’s disease, especially often combined with osteoporosis, have higher risk of osteoporotic fracture. However, large cohort study assessing the preventive effect of bisphosphonates for osteoporotic fracture on patients with Parkinson’s disease is scarce. This retrospective cohort study included nearly whole population of South Korea during 2002 to 2018 from the national patient database of Korea National Health Insurance Service. Of 608,202 patients with Parkinson’s disease, a total of 7,335 patients were selected for index regression model and 5,806 patients were further selected for landmark model of one-year after diagnosis of osteoporosis. Occurrence of osteoporotic fracture was set as outcome variable for both regression models. Compliant use of bisphosphonates showed significant preventive effect for osteoporotic fracture (hazard ratio = 0.83, P = 0.012) in regression analysis with landmark model of one-year after index diagnosis of osteoporosis. In index regression model, higher age, larger waist circumference, smoking, and history of lymphoma were significant risk factors of osteoporotic fractures. Higher weight and higher physical activity score were significant preventative factors. In the regression analysis with landmark model with 5806 patients, higher age, heavy alcohol consumption, smoking, and history of solid tumor were significant risk factors of osteoporotic fractures. Higher weight was a significant preventative factor.
Multiscale off-fault brecciation records coseismic energy budget of principal fault zone
Authors: Agroli Geri , Okamoto Atsushi , Uno Masaoki , Tsuchiya Noriyoshi 
Published: February 2024

Abstract
Breccia and pulverized rock are typical textures in off-fault damage adjacent to a main seismogenic zone. Previously, by estimating the energy required to advance the rupture in this zone using particle size distribution at sub-millimeter/micrometer scales, we could constrain the energy budget during coseismic events. However, whether microscopic estimation is sufficient to capture surface energy fragmentation during an earthquake and the effect of measurement scale variation on calculation of co-seismic energy partitioning remained unclear. Here, we investigated the mechanism of coseismic off-fault damage based on field and microstructural observations of a well-exposed breccia body in Ichinokawa, Japan. We used in situ clast measurements coupled with thin-section analysis of breccia clasts to estimate the energy budget of the damage zone adjacent to the principal slip zone of the median tectonic line. The total surface energy density and corresponding surface energy per unit fault for a width of ~ 500 m of the dynamical damage zone were estimated. The moment magnitude estimated based on surface energy was 5.8–8.3 Mw. In Ichinokawa, off-fault fragmentation is initiated by coseismic activity and is followed by fluid activity. Under dynamic fragmentation conditions, the scale is important to calculate the surface energy.
Genetically predicted body mass index and prostate cancer risk
Authors: Minjun Xie , Xiuyun Wen , Xiangtao Weng , tao xiang 
Published: February 2024

Abstract
Objectives
We employ network Mendelian randomization (MR) to investigate the causal relationship between body mass index (BMI) and prostate cancer, while concurrently examining the potential mediating role of both bioavailable and total testosterone.
Methods
The PRACTICAL consortium is used for prostate cancer. Data from GIANT consortium are used for BMI. The Inverse variance weighted (IVW), MR Egger, Weighted median, and Weighted mode methods are used to evaluate evidence for a causal relationship. The BMI-prostate cancer, BMI-testosterone, and testosterone-prostate cancer associations are analyzed.
Results
In the IVW method, it shows that BMI will decrease prostate cancer risk (odds ratio, 0.822; 95% confidence interval, 0.738–0.915; P < 0.001), proving that genetically predicted BMI has a negative causality with prostate cancer. BMI is causally negatively associated with bioavailable testosterone (in the IVW method, Beta, -0.146; SE, 0.041; P < 0.001) and total testosterone (in the IVW method, Beta, -0.210; SE, 0.047; P < 0.001). Yet, only higher bioavailable testosterone level is positively associated with an increased prostate cancer risk.
Conclusion
Our results support evidence for an inverse association between genetic predicted BMI and prostate cancer. Our study also suggests the hypothesis that bioavailable testosterone but not total testosterone may mediate the inverse association from BMI to prostate cancer.
Survival and Risk Factor Analysis in Patients with Septic Arthritis: A Retrospective Study of 192 Cases
Authors: Schindler Melanie , Huber Lorenz , Walter Nike , Straub Josina , Lang Siegmund , Szymski Dominik , Bärtl Susanne , Alt Volker , Rupp Markus 
Published: February 2024

Abstract
Background
Septic arthritis (SA) poses a complex clinical puzzle and is associated with significant levels of morbidity and mortality. The objective of this study was (1) to explore overall mortality rates and (2) analyze and identify possible factors that might contribute to the risk of death in patients afflicted with SA.
Methods
This retrospective study focused on patients suffering from SA who received treatment at a German university hospital between January 01, 2011 to December, 31 2021. Identification of patients was carried out through International Classification of Diseases (ICD)-10 diagnosis codes specifically related to SA, denoted as "M00.-". The study analyzed the overall mortality rate as well as comorbidities, and pathogens as potential risk factors. Kaplan–Meier probability plots and odds ratios (OR) for mortality were calculated.
Results
In a cohort of 192 patients suffering from SA, 64 patients (33.3%) passed away during a mean follow-up time of 54.4 ± 42 months. The overall mortality was 17.5% at one year, 19.9% at two years and 28.3% at five years. Patients being 65 years or older (p < 0.001), arterial hypertension (p = 0.003), congestive heart failure (p = 0.001), chronic renal disease (p < 0.001), chronic liver disease (p = 0.013), peripheral vascular disease (p = 0.026), malignancy (p < 0.001), steroid use (p < 0.001), immunosuppression (p = 0.003) and intraabdominal infection (p = 0.049) demonstrated a significant higher rate of mortality. Comorbidities were found to be associated with mortality. Chronic renal disease (OR = 2.80; p = 0.000), malignancy (OR = 3.40; p = 0.006) and chronic heart failure (OR = 2.62; p = 0.039).
Conclusion
The vulnerable patient group demonstrated a notably elevated mortality rate. Understanding the intricate interplay of factors contributing to mortality is paramount. Prompt evaluation and addressing of individualized risk factors in the early stages could offer significant advantages in effectively managing and treating SA, thereby lowering the chances of mortality. These results underscore the critical need for vigilant monitoring of SA patients who have pre-existing chronic organ conditions, timely recognition, and prompt intervention for sepsis.
Cost-Utility Analysis of Prenatal Diagnosis of Congenital Cardiac Diseases using Deep Learning
Authors: Ginsberg Gary Michael , Drukker Lior , Pollak Uri , Brezis Meyer 
Published: February 2024

Abstract
Background
Deep learning (DL) is a new technology that can assist prenatal ultrasound (US) in the detection of congenital heart disease (CHD) at the prenatal stage. Hence, an economic-epidemiologic evaluation (aka Cost-Utility Analysis) is required to assist policymakers in deciding whether to adopt the new technology.
Methods
The cost-utility ratios (CUR) were calculated for the current provision of US plus pulse oximetry (POX),and with DL-assisted ultrasound (DL-US) plus POX by means of a spreadsheet model integrating demographic, economic epidemiological, health service utilization, screening performance, survival and lifetime quality of life data based on the standard formula:
CUR = (Intervention Costs - Treatment Savings)/ Quality Adjusted Life Years (QALY) gained
US screening data were based on data from real-world operational routine reports (as opposed to research studies). The DL screening cost of 145 USD was based on Israeli US costs plus 20.54 USD for reading and recording screens.
Results
The addition of DL-US, which is associated with increased sensitivity (95% vs 58.1%), resulted in far fewer undiagnosed infants (16 vs 102 [or 2.9% vs 15.4% of the 560 and 659 births, respectively). Adoption of DL-US will add 1,204 QALYs. The increased screening costs of DL-US (23.2 million USD) are largely offset by decreased treatment costs (20.8 million NIS). Therefore, the new DL-US technology is considered “very cost-effective”, costing only 6,441 NIS per QALY. For most performance combinations (sensitivity > 80%, specificity >90%), the adoption of DL-US is either cost effective or very cost- effective. For specificities greater than 98% (with sensitivities above 94%),DL-US (& POX) is said to “dominate” US (& POX) by providing more QALYs at a lower cost.
Conclusion
Our exploratory CUA calculations indicate the feasibility of DL-US as being at least cost-effective.
Integrated morphometric and molecular approaches to screen hybrid from wild Labeo rohita and Catla catla parent populations
Authors: Lenka Pritam , Singh Namrata , Ghosh Deepra , Mahadani Pradosh , Ghosh Sajalendu 
Published: February 2024

Abstract
Background: Interspecific hybrids of rohu (Labeo rohita) and catla (Catla catla) are common, especially in India due to constrained breeding. These hybrids must segregate from their wild parents as part of conservational strategies. In this study, we screen the hybrid from wild Labeo rohita and Catla catla parents using both morphometric and molecular approaches.
Methods & Results:The carp samples were collected from Jharkhand and West Bengal, India. The correlation and regression analysis of morphometric features are considered superficial but could be protracted statistically by clustering analysis and further consolidated by nucleotide variations of one mitochondrial and one nuclear gene to differentiate hybrids from their parents. Out of twenty morphometric features, six were used for clustering analysis. The clustering analysis showed discrete separation among rohu, catla, and their hybrids when the data points were plotted in a low-dimensional 2-D plane using the first 2 principal components. Out of forty selected SNP positions of the COX1 gene, hybrid showed 100% similarity with catla. Concerning SNP similarity of the 18S rRNA nuclear gene, the hybrid showed 100 % similarity with rohu but not with catla thus exhibiting its most probable paternal origin as rohu that confirms their parental source of sperm (rohu) and ovum (catla).
Conclusions: Along with clustering analysis the SNP comparison study together points towards strong evidence of interspecific hybridization between rohu and catla, as these hybrids share both morphological and molecular differences with either parent. However, this screening process will be further consolidated by analysing more sequences.
Multiplex gradient immunochip for detection of post-vaccinal antibodies in poultry
Authors: Saushkin Nikolay , Samsonova Jeanne , Presnova Galina , Rubtsova Maya , Osipov Alexander 
Published: February 2024

Abstract
Multiplex analysis as an immunochip-in-a well format for simultaneous detection of post-vaccinal antibodies to three poultry infections (Newcastle disease, infectious bronchitis and bursal disease) in one chicken sera was developed. The immunochip had a microarray format printed on the bottom of a standard microtiter plate well and consisted of 36 microspots (d = 400 µm each) with three lines of viral antigens absorbed in a gradient of five decreasing concentrations. Optimization of assay conditions revealed the necessity of careful choice of the reaction buffer due to the high tendency of chicken IgY to exhibit unspecific binding. Assay results were visualized by a number of coloured microspots that were correlated with the specific antibody titre in the analysed serum. High, medium or low antibody titre level for each of three infections could be quickly assessed visually or with the help of smartphone. ELISA results (antibody titres) and visual gradient immunochip results interpretation (high, medium, low antibody level/titre) for 63 chicken sera with multiple levels of post-vaccinal antibodies against Newcastle disease, infectious bronchitis and bursal disease were in good correlation.
3D Printing in Clinical Laryngology: A Scoping Review
Authors: Cameron Nicholas , Jiang Megan , Rohmiller Amy , Dion Gregory 
Published: February 2024

Abstract
Background: Although many 3D printed devices and implants are being integrated into clinical practice, 3D printing is variably employed in the field of laryngology. With its increased use in bench and preclinical work, an up-to-date review of available technologies and implementation in clinical laryngology practice is needed.
Review Methods: A comprehensive literature search following review guidelines and with the assistance of a medical librarian in November 2022 identified 1,870 articles related to 3D printing in laryngology. Data sources were PubMed and Web of Science. After excluding pre-clinical, simulation, and bench-top work, twenty articles were selected for full-text review and five were included in the final analysis.
Results: Four studies created a bioresorbable external airway splint for the indication of severe tracheobronchomalacia not amendable to current, best available treatment methods, and the fifth study created a 3D printed titanium mesh for thyroid cartilage reconstruction after partial laryngectomy. All included studies were published in either the United States or China between 2004 and 2022. Three studies were performed in the pediatric population and two were performed in adults.
Conclusion: Clinical applications of 3D printing within laryngology are currently rare but are promising for the future of laryngotracheal interventions and reconstruction. Although most studies are still preclinical, awareness of the current state of the clinical science is crucial for future developments and clinical trials.
Effects of CCT subunits on clinical prognosis and tumor microenvironment in thyroid cancer
Authors: Bai Yunfeng , Shi Xiaohui 
Published: February 2024

Abstract
To investigate the expression, clinical significance, mechanism of action, and tumor microenvironment (TME) of eight subunits of the chaperonin-containing TCP1 complex (CCT/TRiC) in thyroid cancer (TC). Methods:The Cancer Genome Atlas (TCGA) and high-throughput functional genome (GEO) databases were used to analyze the differential expression of each CCT subunit in TC and normal tissues, and TCGA and Gene Expression Profiling Interaction Analysis (GEPIA) were used to analyze the effects of the expression levels of each CCT subunit on patient prognosis and the construction of survival risk models. Gene Enrichment Analysis (GSEA) was used to analyze the CCT of each subunit's biological functions, and the effects of each CCT subunit on the TC tumor microenvironment, drug sensitivity, and immune checkpoints were analyzed using TCGA and TCGA Tumor Immune Infiltration Analysis Database (TIMER2.0). Further analysis was performed to analyze the correlation between the expression of each CCT subunit and immune cell infiltration of TCs and drug targets. Results:There was a significant difference was found in the expression of each CCT subunit between the TC and normal thyroid tissues (P < 0.05). The subunits of CCT affect the prognosis of TC and are closely related to the TME, immune cell infiltration, immune checkpoints, and drug sensitivity. Conclusions:CCT complexes are potential molecular biomarkers for thyroid cancer, which may promote thyroid cancer development by affecting the tumor microenvironment, thus influencing patient prognosis. They also have the potential to serve as targets for diagnosis and immunotherapy of refractory thyroid cancer.
Development, Content Validation and Pilot Study Feasibility of Heartfulness Meditation and Yoga Protocol for Anxiety
Authors: Thakur Mansee 
Published: February 2024

Abstract
Integrating contemplative practices such as meditation and yoga may offer a comprehensive approach for the rise in mental health issues. We aimed to develop and validate a Heartfulness Meditation and Yoga protocol for individuals with low to moderate anxiety. In Phase 1, we developed the protocol from extensive literature survey using classical yoga texts and scientific studies. Phase 2 involved content validation through experts from fields related to yoga, Ayurveda, physiology, psychology, and psychiatry, by calculating the content validity ratio (CVR). Phase 3 consisted of a pilot study to check the feasibility and effectiveness of the protocol. Data were collected using Beck Anxiety Inventory (BAI), WHO-Well Being Index (WHO-WBI) and Perceived Stress Scale (PSS). The final protocol encompassed practices such meditation, relaxation, cleaning and pranayamas. In the validation phase, 40 subject experts participated, and all practices (for low and moderate anxiety) were incorporated in the final protocol, as CVR scores were >0.29. Participants from the pilot study revealed a high satisfaction rate of 90% and significant reduction in anxiety, and improved well-being. Our approach was safe, feasible and effective, making it a promising addition to conventional anxiety treatments. In future, randomized clinical trials are required.
CTRI No. CTRI/2023/11/060151
Os celulares,os professores e os alunos:desafios para a elaboração de um novo contrato pedagógico
Authors: Zuin Antônio Álvaro Soares , Ferreira Damiana Andreia 
Published: February 2024

Na sociedade da chamada cultura digital, os aparelhos celulares se disseminam de uma tal forma que, em muitas ocasiões, são identificados pelas pessoas como se fossem parte de seu próprio corpo. Evidentemente, o ambiente escolar é identificado como um espaço onde tais aparelhos tornam-se onipresentes, a ponto de suscitar a reflexão sobre como eles poderiam ser pedagogicamente utilizados. Diante disso, o objetivo dos autores deste artigo é o de refletir criticamente sobre o papel do professor (a) de nível médio de ensino diante da utilização, cada vez mais frequente, do uso de aparelhos celulares dentro e fora das salas de aula para fins supostamente pedagógico. Apoiando-se nos pensadores da Teoria Crítica da Sociedade, utilizou-se como metodologia a análise de 17 artigos acadêmicos publicados nos últimos, os quais examinaram a contribuição dos celulares em turmas do ensino médio. A partir disso, foi possível concluir que, se o celular pode tanto ser pedagogicamente utilizado, quanto engendrar práticas de dispersão de concentração e cyberbullying, torna-se fundamental fomentar a presença de contratos pedagógicos entre os agentes educacionais para que possam mutuamente consensuar sobre quando e como usar tal aparelho no transcorrer das atividades realizadas nas salas de aula.
Real estate up to the mark: review and analysis of the real estate market in some Gulf countries
Authors: Babenkova S. Yu. 
Published: February 2024

The purchase of real estate by foreigners and its full ownership in the Gulf countries has become feasible only recently. The diversification of the economies of oil exporting countries is forcing them to strengthen the role of non–oil sectors, in particular the real estate sector.A sharp increase in demand for real estate occurred, including two years ago, with the arrival of wealthy Russians on the UAE market, who decided to minimize the risks to their finances by investing in real estate in the Emirates.At the same time, despite the opening windows of opportunity for foreign investors, according to some experts, the UAE market may be overheated and a bubble, characteristic of fast-growing markets, will form, the further development of which may lead to a market decline.