bluuebunny/crossref_metadata_2025_split huggingface dataset:
Male song structure predicts offspring recruitment to the breeding population in a migratory bird
Authors: DiSciullo Rachael A , Forsman Anna M , Fitak Robert R , Hunt John , Nietlisbach Pirmin , Thompson Charles F , Sakaluk Scott K 
Published: March 2024

Abstract
Bird song is a classic example of a sexually selected trait, but much of the work relating individual song components to fitness has not accounted for song typically being composed of multiple, often-correlated components, necessitating a multivariate approach. We explored the role of sexual selection in shaping the complex male song of house wrens (Troglodytes aedon) by simultaneously relating its multiple components to fitness using multivariate selection analysis, which is widely used in insect and anuran studies but not in birds. The analysis revealed significant variation in the form and strength of selection acting on song across different selection episodes, from nest-site defense to recruitment of offspring to the breeding population. Males that sang more song typically employed in close communication sired more offspring that were subsequently recruited to the breeding population than those that sang more far-communication song. However, this relationship was not consistent across earlier selection episodes, as evidenced by non-linear selection acting on these song components in other contexts. Collectively, our results present a complex picture of multivariate selection on male song structure that would not be evident using univariate approaches and suggest possible trade-offs within and among song components at different points of the breeding season.
Translating evidence into practice in primary care management of adolescents and women with polycystic ovary syndrome: a mixed-methods study
Authors: Cowan Stephanie , Moran Lisa , Garad Rhonda , Sturgiss Elizabeth , Lim Siew , Ee Carolyn 
Published: March 2024

Abstract

Background
The international guideline on polycystic ovary syndrome (PCOS) provides evidence-based recommendations on the management of PCOS. Guideline implementation tools (GItools) were developed for general practitioner (GP) use to aid rapid translation of guidelines into practice. This mixed-methods study aimed to evaluate barriers and enablers of the uptake of PCOS GItools in general practice.

Design and setting
A cross-sectional survey was distributed through professional networks and social media to GPs and GPs in training in Australia. Survey respondents were invited to contribute to semi-structured interviews. Interviews were audio-recorded and transcribed verbatim. Qualitative data were thematically analysed and mapped deductively to the Theoretical Domains Framework and Capability, Opportunity, Motivation and Behaviour model.

Results
The study engaged 146 GPs through surveys, supplemented by interviews with 14 participants. A key enabler to capability was reflective practice. Barriers relating to opportunity included limited awareness and difficulty locating and using GItools due to length and lack of integration into practice software, while enablers included ensuring recommendations were relevant to GP scope of practice. Enablers relevant to motivation included co-use with patients, and evidence of improved outcomes with the use of GItools.

Discussion
This study highlights inherent barriers within the Australian healthcare system that hinder GPs from integrating evidence for PCOS. Findings will underpin behaviour change interventions to assist GPs in effectively utilising guidelines in clinical practice, therefore minimising variations in care. While our findings will have a direct influence on guideline translation initiatives, changes at organisational and policy levels are also needed to address identified barriers.
Tree belowground biomass in Congo Basin forests: allometric equations and scaling with aboveground biomass
Authors: Kossi Ditsouga Alain Franck , Moundounga Mavouroulou Quentin , Moundounga Cynel Gwenael , Fayolle Adeline , Picard Nicolas , Sato Akinobu , Ngomanda Alfred 
Published: March 2024

Abstract
Many allometric models to predict tree aboveground biomass have been developed in tropical moist forests, but few models are available for tree belowground biomass. Theory predicts that belowground biomass scales in an isometric way with aboveground biomass. Estimates of belowground biomass could then be derived from aboveground biomass using the root:shoot ratio. Using a dataset of 118 tropical trees for which both aboveground and belowground biomass and other tree and species characteristics were measured in Gabon and Cameroon, we found a near isometric, yet significantly allometric, relationship between belowground biomass (B, in kilograms) and aboveground biomass (A, in kilograms): B = 0.324 A0.939. The root:shoot ratio was 0.20–0.22, regardless of tree size. An efficient model to predict belowground biomass from tree diameter (D, in centimeters), height (H, in meters) and wood density (ρ, in grams per cubic centimeter) was B = 0.0188 (ρD2H)0.977. A significant residual effect of species and leaf habit was found in this model, indicating that further tree and species characteristics are likely to explain additional variation in belowground biomass. Yet, the future development of belowground allometric models can benefit from the many models already developed for aboveground biomass. On the basis of this unprecedented sampling effort on tree belowground biomass in the dense tropical forests of the Congo Basin, we conclude that the scaling of belowground biomass with aboveground biomass should be the relationship to focus on.
The significant role of glycosaminoglycans in tooth development
Authors: Inubushi Toshihiro , Nag Priyanka , Sasaki Jun-Ichi , Shiraishi Yuki , Yamashiro Takashi 
Published: March 2024

Abstract
This review delves into the roles of glycosaminoglycans (GAGs), integral components of proteoglycans, in tooth development. Proteoglycans consist of a core protein linked to GAG chains, comprised of repeating disaccharide units. GAGs are classified into several types, such as hyaluronic acid, heparan sulfate, chondroitin sulfate, dermatan sulfate, and keratan sulfate. Functioning as critical macromolecular components within the dental basement membrane, these GAGs facilitate cell adhesion and aggregation, and play key roles in regulating cell proliferation and differentiation, thereby significantly influencing tooth morphogenesis. Notably, our recent research has identified the hyaluronan-degrading enzyme Transmembrane protein 2 (Tmem2) and we have conducted functional analyses using mouse models. These studies have unveiled the essential role of Tmem2-mediated hyaluronan degradation and its involvement in hyaluronan-mediated cell adhesion during tooth formation. This review provides a comprehensive summary of the current understanding of GAG functions in tooth development, integrating insights from recent research, and discusses future directions in this field.
Heavy is the crown: CEOs’ social interactions and layoff decisions
Authors: Bassanini Andrea , Caroli Eve , Geay Kevin , Reberioux Antoine 
Published: March 2024

Abstract
We develop a theory of non-monetary costs incurred by chief executive officers (CEOs) when deciding about layoffs and test its predictions on French data. Our results support the idea that, being embedded in their social environment, CEOs find it more difficult to fire employees closer to their own workplace. This effect is stronger whenever social interactions are less anonymous in the CEOs’ local environment. It is weaker when CEOs live further away from where they work, because of limited exposure to local discontent.
Media Exposure, Political Predispositions, and Conspiracy Theories: Explaining Chinese Beliefs in COVID Origins
Authors: Ma Deyong , Lu Ming 
Published: March 2024

Abstract
This article examines the underpinning mechanisms of the Chinese public’s belief in two opposing conspiracy theories on the origins of coronavirus disease 2019. Utilizing survey data from 4,251 Chinese internet users, our study reveals both the universality and distinctiveness of mechanisms involved in conspiracy theory beliefs. The distinctiveness primarily emanates from the political system and its media environment, while universality is mainly attributed to the cognitive style of intuitive thinking. In China’s political system, exposure to either domestic or foreign media sources exerts substantial influence on public conspiracy beliefs. Notably, we emphasize the mediating role of political ideologies in domestic media exposure. Exposure to domestic media is more likely to lead conservative individuals to reject the conspiracy asserting COVID-19 originated in a lab in China. Concurrently, nationalism mediates foreign media exposure, reinforcing convictions of U.S.-centered conspiracies. This research shows how media narratives, molded by the political context, generate divergent perspectives on conspiracy theories in China. Furthermore, given the role of intuitive thinking in conspiracy beliefs, our findings highlight the potential for cultivating a more rational society through the promotion of analytical thinking, which requires the public to employ a deliberative and conscious cognitive process when exposed to highly politicized media content.
A Novel Anti-nucleocapsid Antibody Avidity Method for Identifying SARS-CoV-2 Reinfections
Authors: Golding Liam , Watts Allison W , Shew Jacob , Viñeta Paramo Marina , Mâsse Louise C , Goldfarb David M , Abu-Raya Bahaa , Lavoie Pascal M 
Published: March 2024

Abstract
Detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reinfections is challenging with current serology assays and is further complicated by the marked decrease in routine viral testing practices as viral transmission increased during Omicron. Here, we provide proof-of-principle that high-avidity anti-nucleocapsid (N) antibodies detects reinfections after a single infection with higher specificity (85%; 95% confidence interval [95% CI], 80%–90%) compared to anti-N antibody levels (72%; 95% CI, 66%–79%) in a vaccinated cohort. This method could be used to retroactively investigate the epidemiology and incremental long-term health consequences of SARS-CoV-2 reinfections.
An inhibitory immunoreceptor Allergin-1 regulates the intestinal dysbiosis and barrier function in mice
Authors: Lin Yu-Hsien , Tahara-Hanaoka Satoko , Obana Nozomu , Fukuda Shinji , Shibuya Akira 
Published: March 2024

Abstract
The intestinal barrier consists of mucosal, epithelial, and immunological barriers and serves as a dynamic interface between the host and its environment. Disruption of the intestinal barrier integrity is a leading cause of various gastrointestinal diseases, such as inflammatory bowel disease. The homeostasis of the intestinal barrier is tightly regulated by crosstalk between gut microbes and the immune system; however, the implication of the immune system on the imbalance of gut microbes that disrupts barrier integrity remains to be fully elucidated. An inhibitory immunoglobulin-like receptor, Allergin-1, is expressed on mast cells and dendritic cells and inhibits Toll-like receptor (TLR)-2 and TLR-4 signaling in these cells. Since TLRs are major sensors of microbiota and are involved in local epithelial homeostasis, we investigated the role of Allergin-1 in maintaining intestinal homeostasis. Allergin-1-deficient (Milr1−/−) mice exhibited more severe dextran sulfate sodium (DSS)-induced colitis than did wild-type (WT) mice. Milr1−/− mice showed an enhanced intestinal permeability compared with WT mice even before DSS administration. Treatment of Milr1−/− mice with neomycin, but not ampicillin, restored intestinal barrier integrity. The 16S rRNA gene sequencing analysis demonstrated that Bifidobacterium pseudolongum was the dominant bacterium in Milr1−/− mice after treatment with ampicillin. Although the transfer of B. pseudolongum to germ-free WT mice had no effect on intestinal permeability, its transfer into ampicillin-treated WT mice enhanced intestinal permeability. These results demonstrated that Allergin-1 deficiency enhanced intestinal dysbiosis with expanded B. pseudolongum, which contributes to intestinal barrier dysfunction in collaboration with neomycin-sensitive and ampicillin-resistant microbiota.
Antibiotic prescribing patterns and carriage of antibiotic-resistant Escherichia coli and Enterococcus species in healthy individuals from selected communities in Lusaka and Ndola districts, Zambia
Authors: Yamba Kaunda , Mudenda Steward , Mpabalwani Evans , Mainda Geoffrey , Mukuma Mercy , Samutela Mulemba Tillika , Lukwesa Chileshe , Chizimu Joseph , Kaluba Ciluvya Kavimba , Mutalange Matenge , Chilengi Roma , Muma John Bwalya 
Published: March 2024

Abstract

Objectives
This study assessed antibiotic prescribing patterns in primary healthcare facilities and antimicrobial resistance (AMR) profiles of commensal Escherichia coli and enterococci isolated from pregnant women and children under 5 years of age.

Materials and methods
This cross-sectional study was conducted in Lusaka and Ndola districts of Zambia. Prescription pattern data were obtained from hospital pharmacies. Identification and antimicrobial susceptibility profiles of E. coli and enterococci were determined by conventional methods, while confirmation of both pathogens and AMR genes were determined by PCR. Data were analysed using WHONET and SPSS version 25.0.

Results
Most prescribed antibiotics at the primary healthcare facilities belonged to the Access group of the WHO Access, Watch and Reserve (AWaRe) classification. All the primary healthcare facilities adhered to the AWaRe framework of ≥60% prescribed antibiotics belonging to the Access group. However, resistance was highest in the Access group of antibiotics. E. coli resistance to ampicillin ranged from 71% to 77% and to co-trimoxazole from 74% to 80%, while enterococcal resistance to tetracycline was 59%–64%. MDR was highest in E. coli (75%) isolates, while XDR was highest in enterococcal isolates (97%). The identified AMR genes in E. coli included blaCTX-M, sul2 and qnrA, while those of enterococci included erm(B), erm(C) and erm(A).

Conclusions
Resistance was highest in the prescribed WHO Access group of antibiotics. These findings highlight the need to use local susceptibility data to formulate country-specific treatment guidelines in line with WHO AWaRe classification and enforce regulations that prohibit easy access to antibiotics.
Effect of continuous or intermittent feeding of ergot contaminated grain in a mash or pelleted form on the performance and health of feedlot beef steers
Authors: Reynolds Matthew R , Stanford Kim , Meléndez Daniela M , Schwartzkopf-Genswein Karen S , McAllister Tim A , Blakley Barry R , McKinnon John J , Ribeiro Gabriel O 
Published: March 2024

Abstract
This study evaluated the effect of feeding ergot contaminated grain continuously or intermittently through backgrounding (BG) and finishing (FN) in a mash or pelleted supplement on the growth performance, health and welfare parameters, and carcass characteristics of feedlot beef steers. Sixty black Angus steers (300 ± 29.4 kg BW) were used in a complete randomized 238-d study. Steers were stratified by weight and randomly assigned to four different diets (15 steers/treatment) and individually housed. Treatments included: (1) control [CON; no added ergot alkaloids (EA)], (2) continuous ergot mash (CEM; fed continuously at 2 mg total EA/kg of DM), (3) intermittent ergot mash (IEM; fed at 2 mg total EA/kg of DM, during the first week of each 21-d period and CON for the remaining 2 wk, this feeding pattern was repeated in each period), and (4) intermittent ergot pellet (IEP; fed at 2 mg of total EA/kg of DM as a pellet during the first week of each 21-d period and CON for the remaining 2 wk as described for IEM). Steers were fed barley based BG diets containing 40% concentrate:60% silage (DM basis) for 84 d (four 21-d periods), transitioned over 28 d (no ergot fed) to an FN diet (90% concentrate:10% silage DM basis) and fed for 126 d (six 21-d periods) before slaughter. In the BG phase, steer DMI (P &lt; 0.01, 7.45 vs. 8.05 kg/d) and ADG (P &lt; 0.01) were reduced for all EA diets compared to CON. The CEM fed steers had lower ADG (P &lt; 0.01, 0.735 vs. 0.980 kg) and shrunk final BW (P &lt; 0.01, 350 vs. 366 kg) than CON. CEM had lower gain:feed (P &lt; 0.07, 0.130 vs. 0.142) than CON. In the FN phase, steer DMI (P &lt; 0.01, 9.95 vs. 11.05 kg/d) and ADG (P = 0.04) were also decreased for all EA fed steers compared to CON. Total shrunk BW gain (P = 0.03, 202.5 vs. 225.2 kg), final BW (P = 0.03, 617.9 vs. 662.2 kg), and carcass weight (P = 0.06) decreased for all EA fed steers compared to CON. The percentage of AAA carcasses decreased for all EA fed steers (P &lt; 0.01, 46.7 vs. 93.3%) compared to CON. EA fed steers had increased rectal temperatures (P &lt; 0.01, 39.8 vs. 39.4 °C) compared to CON. Pelleting ergot contaminated grain did not reduce the impact of ergot alkaloids on any of the measured parameters during BG or FN. Continuously or intermittently feeding ergot contaminated diets (2 mg total EA/kg of DM) significantly reduced intake, growth performance, and carcass weight, with minimal impact on blood parameters in feedlot steers. Pelleting was not an effective method of reducing ergot toxicity.
FATP2 regulates osteoclastogenesis by increasing lipid metabolism and ROS production
Authors: Kong Xiangxi , Tao Siyue , Ji Zhongyin , Li Jie , Li Hui , Jin Jiayan , Zhao Yihao , Liu Junhui , Zhao Fengdong , Chen Jian , Feng Zhenhua , Chen Binhui , Shan Zhi 
Published: March 2024

Abstract
Lipid metabolism plays a crucial role in maintaining bone homeostasis, particularly in osteoclasts (OCs) formation. Here, we found that the expression level of FATP2, a transporter for long-chain and very-long-chain fatty acids, was significantly upregulated during OC differentiation and in the bone marrow of mice fed a high-fat diet (HFD). Notably, the use of FATP2 siRNA or a specific inhibitor (Lipofermata) resulted in significant inhibition of OC differentiation, while only slightly affecting osteoblasts. In pathological models of bone loss induced by LPS or ovariectomy, in vivo treatment with Lipofermata was able to rescue the loss of bone mass by inhibiting OC differentiation. RNA sequencing revealed that Lipofermata reduced fatty acid β-oxidation and inhibited energy metabolism, while regulating ROS metabolism to decrease ROS production, ultimately inhibiting OC differentiation. Treatment with Lipofermata, either in vivo or in vitro, effectively rescued the overactivation of OCs, indicating that FATP2 regulated OC differentiation by modulating fatty acid uptake and energy metabolism. These findings suggested that targeting FATP2 may represent a promising therapeutic approach for pathological osteoporosis.
Providing rigor in bee colony strength auditing methods
Authors: Cook Daniel , Hauxwell Caroline 
Published: March 2024

Abstract
The primary method used to audit honey bee (Apis mellifera Linnaeus, 1758 [Hymenoptera: Apidae]) colony strength for almond pollination services, Nasr et al.’s (1990) frame-top cluster count method, is a subjective visual audit that relies on an auditor’s spot assessment and may lack rigor and repeatability. We created novel, open-source software for the analysis of frame-top cluster count photographic assessments to improve methodological rigor and repeatability. We evaluated 2 existing visual audit methods, created 3 novel audit method variations, and determined between-method conversion factors using linear modeling. The software has potential applications in apiological research, apiarist and orchardist colony auditing, as well as training future generations of apiarists in auditing techniques. The software enhances the rigor and repeatability of Nasr et al.’s (1990) frame-top cluster count population assessment. In this article, we introduce the novel open-source software and between-method regression equations and review the tested visual assessment methods and their application.
Mitochondrial genomes of Nemourinae species (Plecoptera: Nemouridae) and the phylogenetic implications
Authors: Wang Ying , Guo Caiyue , Yue Xiaoxiao , Fan Xing , Fan Yuying , Cao Jinjun 
Published: March 2024

Abstract
Currently, the classification system of 2 subfamilies within Nemouridae has been widely accepted. However, monophyly of 2 subfamilies has not been well supported by molecular evidence. To date, only mitogenomes from genus Nemoura of the subfamily Nemourinae were used in previous phylogenetic studies and produced conflicting results with morphological studies. Herein, we analyzed mitogenomes of 3 Nemourinae species to reveal their mitogenomic characteristics and to examine genus-level classification among Nemouridae. In this study, the genome organization of 3 mitogenomes is highly conserved in gene order, nucleotide composition, codon usage, and amino acid composition. In 3 Nemourinae species, there is a high variation in nucleotide diversity among the 13 protein-coding genes (PCGs). The Ka/Ks values for all PCGs were far lower than 1, indicating that these genes were evolving under purifying selection. The phylogenetic analyses highly support Nemurella as the sister group to Ostrocerca. Meanwhile, Nemoura is recovered as the sister group of Malenka; they are grouped with other Amphinemurinae and emerged from a paraphyletic Nemourinae. More molecular data from different taxonomic groups are needed to understand stoneflies phylogeny and evolution.