bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving multi-pinhole CZT myocardial perfusion imaging specificity without changing sensibility by using adapted filter parameters
Authors: Vauchot Fabien , Dubois Julien , Bourdon Aurélie 
Published: March 2024

Abstract
Background
Meta-analysis show the diagnostic performance of cardiac dedicated multi-pinhole cadmium-zinc-telluride myocardial perfusion imaging (MPI) with a sensibility around 0.9 and a specificity around 0.7. The aim of the present study is to explore a simple method to generate less artefact on MPI using single photon emission computed tomography (SPECT) and to enhance specificity without changing sensibility.

Results
From October 2018 to March 2019, 200 patients who underwent SPECT with [99mTc]Tc-tetrofosmin were prospectively recruited: 100 patients with ischemia or necrosis diagnosis (first arm), and 100 patients with myocardial reversible SPECT artefact (second arm). Each SPECT was explored using two image process based on a Butterworth prefilter and post-filter: the original image processing (reconstruction A) with a cut-off frequency equals to 37% of the Nyquist frequency and order equals to 7, and a second image processing (reconstruction B) with a cut-off frequency equals to 25% of the Nyquist frequency and order equals to 5. For each patient, sum stress or rest score with and without septum (SSRS and SSRSws) were calculated with the two reconstructions. No significant statistical difference between SSRSa and SSRSb was identified for the first arm (P = 0.54) and the relative difference ∆r was − 0.5 ± 11.1% (95% CI − 2.7 to 1.7). We found a significant statistical difference between SSRSa and SSRSb for the second arm (p < 0.0001) and the relative difference ∆r was 69.7 ± 16.2% (95% CI 66.6–72.9).

Conclusion
In conclusion, using a Butterworth prefilter and post-filter cut-off frequency equal to 25% of the Nyquist frequency before iterative reconstruction generates less artefact and improves myocardial SPECT specificity without affecting sensibility compared with the original reconstruction.
Evaluation of the theranostic potential of [64Cu]CuCl2 in glioblastoma spheroids
Authors: Pinto Catarina I. G. , Branco André D. M. , Bucar Sara , Fonseca Alexandra , Abrunhosa Antero J. , da Silva Cláudia L. , Guerreiro Joana F. , Mendes Filipa 
Published: March 2024

Abstract
Background
Glioblastoma is an extremely aggressive malignant tumor with a very poor prognosis. Due to the increased proliferation rate of glioblastoma, there is the development of hypoxic regions, characterized by an increased concentration of copper (Cu). Considering this, 64Cu has attracted attention as a possible theranostic radionuclide for glioblastoma. In particular, [64Cu]CuCl2 accumulates in glioblastoma, being considered a suitable agent for positron emission tomography. Here, we explore further the theranostic potential of [64Cu]CuCl2, by studying its therapeutic effects in advanced three-dimensional glioblastoma cellular models. First, we established spheroids from three glioblastoma (T98G, U373, and U87) and a non-tumoral astrocytic cell line. Then, we evaluated the therapeutic responses of spheroids to [64Cu]CuCl2 exposure by analyzing spheroids' growth, viability, and cells' proliferative capacity. Afterward, we studied possible mechanisms responsible for the therapeutic outcomes, including the uptake of 64Cu, the expression levels of a copper transporter (CTR1), the presence of a cancer stem cell population, and the production of reactive oxygen species (ROS).

Results
Results revealed that [64Cu]CuCl2 is able to significantly reduce spheroids' growth and viability, while also affecting cells' proliferation capacity. The uptake of 64Cu, the presence of cancer stem-like cells and the production of ROS were in accordance with the therapeutic response. However, expression levels of CTR1 were not in agreement with uptake levels, revealing that other mechanisms could be involved in the uptake of 64Cu.

Conclusions
Overall, our results further support [64Cu]CuCl2 potential as a theranostic agent for glioblastoma, unveiling potential mechanisms that could be involved in the therapeutic response.
Caputo fractional backward stochastic differential equations driven by fractional Brownian motion with delayed generator
Authors: Shao Yunze , Du Junjie , Li Xiaofei , Tan Yuru , Song Jia 
Published: March 2024

AbstractOver the years, the research of backward stochastic differential equations (BSDEs) has come a long way. As a extension of the BSDEs, the BSDEs with time delay have played a major role in the stochastic optimal control, financial risk, insurance management, pricing, and hedging. In this paper, we study a class of BSDEs with time-delay generators driven by Caputo fractional derivatives. In contrast to conventional BSDEs, in this class of equations, the generator is also affected by the past values of solutions. Under the Lipschitz condition and some new assumptions, we present a theorem on the existence and uniqueness of solutions.
Temporal and spatial distribution characteristics of the Ming Great Wall
Authors: Yang Yang , Zhang Yukun , Li Yan 
Published: March 2024

Abstract
The Great Wall during the Ming Dynasty (1368–1644) was the most prominent engineering volume and the most perfect military defense structure in the history of China. By combining traditional historical textual research, site surveys, and GIS database analysis, this paper reveals the Ming Great Wall’s spatial and temporal distribution characteristics and the immediate causes of their formation are discussed in the nine military districts of the frontier. The results show that: (1) The general rule of the Ming Great Wall was to set up military forts, passes, piers, and other related defensive facilities before constructing the continuous wall. (2) The Great Wall spatial and temporal distribution of the phases is relatively apparent, divided into five construction phases in the Ming Dynasty (1368–1620): Hongwu to Xuande (1368–1435), unbuilt the wall; Zhengtong to Tianshun (1436–1464), the consecutive wall appeared; Chenghua to Zhengde (1465–1521), the first construction climax of the Great Wall; Jiajing period (1522–1566), the second construction climax of the Great Wall; Longqing to Wanli (1567–1620), the completion of the whole line of the Great Wall.(3)The warfare and defense posture was the immediate factor that prompted the construction of the Great Wall between the Ming Dynasty and Mongolia. This study has some reference significance for restoring the integrity and authenticity of the Ming Great Wall, and contribute to the preservation of the cultural heritage of.
Comparative analysis of volatile fingerprints between different geographical origins and ornamental cultivars of white standard Chrysanthemum (Dendranthema grandiflorum)
Authors: Lee Ji Hye , An Jae Min , Kim Yong-Kyoung , Hur Suel Hye , Park Sang Un , Kim Ho Jin 
Published: March 2024

Abstract
Background
White standard Dendranthema grandiflorum (D. grandiflorum) is widely used for ornamental purposes. The volatile organic components (VOCs) of D. grandiflorum vary depending on the geographical origin and cultivar, which influence the market value. Here, we applied headspace extraction coupled with gas chromatography–mass spectrometry (HS–GC–MS) for the first time to determine the different origins (Korea, China, Vietnam) of D. grandiflorum in three genotypes (Baekgang, Jinba, Iwa-no-hakusen) based on volatile fingerprints and to assess the correlation among the metabolites identified in Korean and non-Korean D. grandiflorum.

Results
A total of 41 VOCs, mainly monoterpenoids, sesquiterpenoids, alcohols, and fatty acids, were identified. Principal component analysis showed that instead of geographical origin, genotype influences D. grandiflorum fingerprints. Cultivar discrimination was mainly affected by the metabolites associated with the alpha-terpinyl cation pathway. The orthogonal partial least squares discriminant analysis model achieved 100% and 93.3% accuracies in the calibration and validation sets, respectively. The results of volcano plots and clustering analysis, sesquiterpenoids were significantly more abundant in non-Korean than in Korean, whereas monoterpenoids were abundant in Korean samples.

Conclusions
41 makers confirmed robustness maintenance over two years. These findings can be useful for reliably identifying the geographical origin of D. grandiflorum and providing a comprehensive understanding of VOCs in D. grandiflorum cultivars grown in different countries of origins.

Graphical Abstract
Association of Timely Comprehensive Genomic Profiling With Precision Oncology Treatment Use and Patient Outcomes in Advanced Non–Small-Cell Lung Cancer
Authors: Yorio Jeff , Lofgren Katherine T. , Lee Jessica K. , Tolba Khaled , Oxnard Geoffrey R. , Schrock Alexa B. , Huang Richard S.P. , Brisbin Lorraine 
Published: March 2024

PURPOSE Timely biomarker testing remains out of reach for many patients with advanced non–small-cell lung cancer (aNSCLC). Here, we studied the quality-of-care implications of closing the gap in timely receipt of comprehensive genomic profiling (CGP) to inform first-line (1L) decisions. METHODS Using a real-world clinicogenomic database, we studied testing and 1L treatment patterns in aNSCLC after the approval of pembrolizumab in combination with pemetrexed and carboplatin (May 10, 2017). To estimate the association of timely CGP results with therapy selection and patient outcomes, we identified patients with no previous genomic testing beyond PD-L1 immunohistochemistry and dichotomized patients by whether CGP results were available before or after 1L therapy initiation. RESULTS In total, 2,694 patients were included in the 1L therapy decision impact assessment. Timely CGP increased matched targeted therapy use by 14 percentage points (17% with CGP v 2.8% without) and precision immune checkpoint inhibitor (ICPI) use by 14 percentage points (18% with CGP v 3.9% without). Receipt of timely CGP resulted in an estimated 31 percentage point decrease in ICPI use among ALK/EGFR/RET/ROS1-positive patients at an expected per-patient reduction in ineffective ICPI therapy cost of $13,659.37 with timely CGP to inform 1L treatment selection. Patient benefit of CGP extended to real-world time to therapy discontinuation (median time to therapy discontinuation: 3.9 v 10 months [hazard ratio, HR, 0.54 [95% CI, 0.42 to 0.70]; P = 1.9E-06; adjusted hazard ratio [aHR], 0.50 [95% CI, 0.38 to 0.67]; P = 2.0E-06) in 1L driver-positive patients. This effect was not significant for real-world overall survival (median overall survival: 32 v 29 months [HR, 1.2 [95% CI, 0.84 to 1.67]; P = .33; aHR, 1.4 [95% CI, 0.92 to 1.99]; P = .12). CONCLUSION Timely CGP is associated with the quality of patient care as measured by 1L matched targeted therapy use, time to therapy discontinuation, and avoidance of ineffective, costly ICPIs.
“A Face of Anguish”: Pain and Portraiture in the Civil War Hospital
Authors: Libow Jess 
Published: March 2024

Abstract
This essay traces the visual cultures that emerged around Civil War soldiers’ pain and argues that the method of portraiture has much to offer the field of health humanities. It begins by tracing efforts to capture Civil War soldiers’ pain in both popular and clinical media before turning to hospital accounts by Louisa May Alcott and Walt Whitman that adopt the trope of portraiture in order to make soldiers’ suffering legible to a wider audience. This essay argues that these ekphrastic accounts make visible not only soldiers’ suffering but also the act of observing and interpreting it, and the essay concludes by suggesting that by bringing into focus the process of perceiving another’s pain, the study of portraiture offers an important complement to both the field of narrative medicine and health humanities approaches to studying visual art.
Ouch: Pain, Heard and Referred
Authors: Belling Catherine 
Published: March 2024

Abstract
The communication of pain has been figured, in both the canonical work of Elaine Scarry and in biomedical discourse, as bringing pain up from bodily depths to a surface where it is visibly manifest to others. This study of acute physical pain in three popular American narrative texts and their film adaptations suggests that the oculocentrism of such accounts has elided the significant role of sound, both verbal and nonverbal, in the expression and witnessing of physical pain. Instances of what was classified in the nineteenth century as sensation literature, these three texts produce direct sensory effects. Scott Smith’s The Ruins (2006) is a horror novel, William Goldman’s Marathon Man (1974) a thriller, and Aron Ralston’s Between a Rock and a Hard Place (2004) (film: 127 Hours) an adventure-survival memoir. Reading pain in each of three triple texts—book, film, and the screenplay transmitting pain from one to the other—opens up a new archive of pain texts that promise sensation rather than relief, offering a new perspective on the imaginative—and paradoxically ameliorative—creativity brought into being by the notorious recalcitrance of pain. This essay argues for attention to the protolinguistic pain language of groans and screams; for a poetics of “ouch.”
How important is hidden phenotypic plasticity arising from alternative but converging developmental trajectories, and what limits it?
Authors: Metcalfe Neil B. 
Published: March 2024

ABSTRACT
Developmental plasticity ­– the capacity for a genotype to develop into different phenotypes, depending on the environment – is typically viewed from the perspective of the resulting phenotype. Thus, if development is viewed as a trajectory towards a target, then developmental plasticity allows environmentally induced alterations to the target. However, there can also be variations in the trajectory. This is seen with compensatory responses, for instance where growth accelerates after an earlier period of food shortage, or where investment in sexual ornaments is maintained even when resources are limiting. If the compensation is complete, the adult phenotype can appear ‘normal’ (i.e. the different developmental trajectories converge on the same target). However, alternative trajectories to a common target can have multiple long-term consequences, including altered physiological programming and rates of senescence, possibly owing to trade-offs between allocating resources to the prioritized trait versus to body maintenance. This suggests that plasticity in developmental trajectories towards a common target leads to variation in the resilience and robustness of the adult body. This form of developmental plasticity is far more hidden than plasticity in final adult target, but it may be more common. Here, I discuss the causes, consequences and limitations of these different kinds of plasticity, with a special focus on whether they are likely to be adaptive. I emphasize the need to study plasticity in developmental trajectories, and conclude with suggestions for future research to tease apart the different forms of developmental plasticity and the factors that influence their evolution and expression.
Early-life environmental effects on birds: epigenetics and microbiome as mechanisms underlying long-lasting phenotypic changes
Authors: Ruuskanen Suvi 
Published: March 2024

ABSTRACT
Although the long-lasting effects of variation in early-life environment have been well documented across organisms, the underlying causal mechanisms are only recently starting to be unraveled. Yet understanding the underlying mechanisms of long-lasting effects can help us predict how organisms will respond to changing environments. Birds offer a great system in which to study developmental plasticity and its underlying mechanisms owing to the production of large external eggs and variation in developmental trajectories, combined with a long tradition of applied, physiological, ecological and evolutionary research. Epigenetic changes (such as DNA methylation) have been suggested to be a key mechanism mediating long-lasting effects of the early-life environment across taxa. More recently, changes in the early-life gut microbiome have been identified as another potential mediator of developmental plasticity. As a first step in understanding whether these mechanisms contribute to developmental plasticity in birds, this Review summarizes how changes in early-life environment (both prenatal and postnatal) influence epigenetic markers and the gut microbiome. The literature shows how both early-life biotic (such as resources and social environment) and abiotic (thermal environment and various anthropogenic stressors) factors modify epigenetic markers and the gut microbiome in birds, yet data concerning many other environmental factors are limited. The causal links of these modifications to lasting phenotypic changes are still scarce, but changes in the hypothalamic–pituitary–adrenal axis have been identified as one putative pathway. This Review identifies several knowledge gaps, including data on the long-term effects, stability of the molecular changes, and lack of diversity in the systems studied, and provides directions for future research.
Negative pressure wound therapy with instillation and dwell time in debridement of fibrinous leg ulcers
Authors: Achiti Alexandru , Zenati Nora , Seinturier Christophe , Cracowski Jean-Luc , Blaise Sophie 
Published: March 2024

Objective: In conjunction with appropriate wound care, negative pressure wound therapy with instillation and dwell time (NPWTi-d) may be used as an adjunct therapy for acute or hard-to-heal (chronic) wounds, especially when infected. However, there are very few data on the use of NPWTi-d in the treatment of fibrinous wounds that are difficult to debride mechanically. The main objective of this study was to describe changes in the fibrin area of such wounds, before and after treatment with NPWTi-d. Method: This was a monocentric, observational, prospective pilot study evaluating the NPWTi-d medical device. Eligible patients included in the study were those with hard-to-heal lower limb ulcers who had previously undergone unsuccessful specific debridement treatment for their wound, with failure of manual mechanic debridement for at least six weeks' duration, and whose wounds had a fibrinous surface area of >70% of the total wound surface area. The primary endpoint was the difference in the percentage of fibrinous surface area before and after treatment. Results: A total of 14 patients who received treatment for lower limb ulcers between October 2017 and August 2019 were included in the study. There was a significant shrinkage rate of the fibrinous wound surface between the start and end of treatment (83.6±14.5% and 32.2±19.7%, respectively; p<0.001). Conclusion: This study showed a significant decrease in fibrin area in wounds treated with NPWTi-d, with good tolerance. We believe that NPWTi-d has its place in the multidisciplinary management of patients with hard-to-heal ulcers. Additional randomised studies are required to confirm these findings. Declaration of interest: The authors have no conflicts of interest.
Evaluation of risk factors associated with hard-to-heal wound infection in a tertiary care hospital
Authors: Jain Manisha , Bhogar Kavin , Baral Purabi , Gaind Rajni 
Published: March 2024

Objective: The incidence of hard-to-heal wound infection, especially as a result of multidrug-resistant Gram-negative organisms, has increased in recent years. The reason for the increase is multifactorial and the ability of these pathogenic isolates to form biofilms is one of the important risk factors in wound infection. This study aimed to evaluate the risk factors associated with such cases. Method: This prospective analytical study, conducted over a period of two months, included pus or tissue samples from hospital inpatients with Gram-negative hard-to-heal wound infection. The samples were processed with conventional microbiological techniques. Patient demographic details and the presence of various risk factors were recorded. Biofilm production was detected by tissue culture plate method in the laboratory. The data were analysed using SPSS version 21 (IBM Ltd., US). Results: The experimental cohort comprised 200 patients. Klebsiella spp. was the most common identified organism, followed by Escherichia coli and Pseudomonas spp. Carbapenem resistance was observed in 106 (53%) strains. Almost 66% of the strains showed biofilm formation. On evaluation of associated risk factors, age (p=0.043), presence of biofilms (p=0.0001), diabetes (p=0.002), hypertension (p=0.02) and medical device use (p=0.008) had significant association, whereas sex, previous surgery and prior antibiotic use had no significant impact on the chronicity of the wound. Conclusion: In this study, chronicity of wounds was observed to be associated with multiple risk factors, especially the biofilm-forming ability of the strain. Biofilms are difficult to eradicate and additional measures, such as physical debridement, are important for resolving chronicity. Knowledge about specific risk factors would also allow clinicians a better understanding of the healing process and drive appropriate wound care interventions. Declaration of interest: A grant was received from the Indian Council of Medical Research (ICMR) for this work (grant ID: 2017-02686). The authors have no conflicts of interest to declare.
A service improvement project to improve follow up screening rates of women with gestational diabetes
Authors: Stace Joanna 
Published: March 2024

Diabetes in pregnant women presents significant implications for long-term health. The best way to manage them in practice is outlined here by Joanna Stace A history of Gestational Diabetes Mellitus (GDM) can have significant implications for women's long-term health. Despite these risks, primary care rates for post-natal screening are low. A Service Improvement Project (SIP) was implemented as part of an MSc in Advanced Diabetes Care undertaken at Birmingham City University. Its aims were to improve post-natal follow-up care for women with GDM, reduce the risk of developing T2DM enhancing their long-term health. A key success of the SIP is the improved knowledge of the importance of post-natal screening amongst staff and women. Increased numbers of women being correctly coded and offered post-natal screening highlighting the importance of healthcare integration for better patient outcomes. Work continues to improve referral rates and attendance of the National Diabetes Prevention Programme with the roll out of an Electronic Care Pathway.