bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chromosomal DNA sequences of the Pacific saury genome: versatile resources for fishery science and comparative biology
Authors: Sato Mana , Fukuda Kazuya , Kadota Mitsutaka , Makino-Itou Hatsune , Tatsumi Kaori , Yamauchi Shinya , Kuraku Shigehiro 
Published: March 2024

Abstract
Pacific saury (Cololabis saira) is a commercially important small pelagic fish species in Asia. In this study, we conducted the first-ever whole genome sequencing of this species, with single molecule, real-time (SMRT) sequencing technology. The obtained high-fidelity (HiFi) long-read sequence data, which amount to ~30-folds of its haploid genome size that was measured with quantitative PCR (1.17 Gb), were assembled into contigs. Scaffolding with Hi-C reads yielded a whole genome assembly containing 24 chromosome-scale sequences, with a scaffold N50 length of 47.7 Mb. Screening of repetitive elements including telomeric repeats was performed to characterize possible factors that need to be resolved towards ‘telomere-to-telomere’ sequencing. The larger genome size than in medaka, a close relative in Beloniformes, is at least partly explained by larger repetitive element quantity, which is reflected in more abundant tRNAs, in the Pacific saury genome. Protein-coding regions were predicted using transcriptome data, which resulted in 22,274 components. Retrieval of Pacific saury homologs of aquaporin (AQP) genes known from other teleost fishes validated high completeness and continuity of the genome assembly. These resources are available at https://treethinkers.nig.ac.jp/saira/ and will assist various molecular-level studies in fishery science and comparative biology.
Circulating cell-free DNA-based biomarkers for prognostication and disease monitoring in adrenocortical carcinoma
Authors: Lippert Juliane , Smith Gabrielle , Appenzeller Silke , Landwehr Laura-Sophie , Prete Alessandro , Steinhauer Sonja , Asia Miriam , Urlaub Hanna , Elhassan Yasir S , Kircher Stefan , Arlt Wiebke , Fassnacht Martin , Altieri Barbara , Ronchi Cristina L 
Published: March 2024

Abstract

Objective
Adrenocortical carcinoma (ACC) is a rare aggressive cancer with heterogeneous behaviour. Disease surveillance relies on frequent imaging, which comes with significant radiation exposure. The aim of the study was to investigate the role of circulating cell-free DNA (ccfDNA)-related biomarkers (BMs) for prognostication and monitoring of ACC.

Design and Methods
We investigated 34 patients with ACC and 23 healthy subjects (HSs) as controls. Circulating cell-free DNA was extracted by commercial kits and ccfDNA concentrations were quantified by fluorimeter (BM1). Targeted sequencing was performed using a customized panel of 27 ACC-specific genes. Leucocyte DNA was used to discriminate somatic variants (BM2), while tumour DNA was sequenced in 22/34 cases for comparison. Serial ccfDNA samples were collected during follow-up in 19 ACC patients (median period 9 months) and analysed in relationship with standard radiological imaging.

Results
Circulating cell-free DNA concentrations were higher in ACC than HS (mean ± SD, 1.15 ± 1.56 vs 0.05 ± 0.05 ng/µL, P &lt; .0001), 96% of them being above the cut-off of 0.146 ng/µL (mean HS + 2 SD, positive BM1). At ccfDNA sequencing, 47% of ACC showed at least 1 somatic mutation (positive BM2). A combined ccfDNA-BM score was strongly associated with both progression-free and overall survival (hazard ratio [HR] = 2.63; 95% CI, 1.13-6.13; P = .010, and HR = 5.98; 95% CI, 2.29-15.6; P = .0001, respectively). During disease monitoring, positive BM2 showed the best specificity (100%) and sensitivity (67%) to detect ACC recurrence or progress compared with BM1.

Conclusion
ccfDNA-related BMs are frequently detected in ACC patients and represent a promising, minimally invasive tool to predict clinical outcome and complement surveillance imaging. Our findings will be validated in a larger cohort of ACCs with long-term follow-up.
Raising awareness among the TESOL community about the professional identity tensions of women EFL teachers in Africa
Authors: Koné Kadidja , Kéita Fatoumata , Koita Binta 
Published: March 2024

Abstract
This collaborative autoethnographic study explores how three female university English teachers in critical friendship navigated professional identity tensions related to the ideological biases of male faculty members implying that women do not belong in academia because of their gender and the responsibilities it entails in an African context. In a reflective collaborative autoethnography, the women raised awareness among TESOL practitioners, researchers, and educators of their professional identity tensions, which ranged from frustration to isolation. They also shared critical reflections on how they invested in their careers to navigate their professional identity tensions in a male-dominated higher-education environment.
Impact of pre-admission physical activity on benefits of physiology-guided complete revascularization in older patients with myocardial infarction: insights from the FIRE trial
Authors: Pavasini Rita , Campo Gianluca , Serenelli Matteo , Tonet Elisabetta , Guiducci Vincenzo , Escaned Javier , Moreno Raul , Casella Gianni , Cavazza Caterina , Varbella Ferdinando , Sacchetta Giorgio , Arena Marco , Santos Ignacio Amat , Ibañes Enrique Gutiérrez , Scarsini Roberto , D’Amico Gianpiero , Ruiz-Poveda Fernando Lozano , Díez Gil José Luis , Pignatelli Gianluca , Iannopollo Gianmarco , Colaiori Iginio , Santos Ramon Calvino , Marrone Andrea , Fileti Luca , Rigattieri Stefano , Barbato Emanuele , Ocaranza-Sanchez Raymundo , Biscaglia Simone 
Published: March 2024

Abstract

Aims
The present analysis from the Functional Assessment in Elderly Myocardial Infarction Patients with Multivessel Disease (FIRE) trial aims to explore the significance of pre-admission physical activity and assess whether the benefits of physiology-guided complete revascularization apply consistently to sedentary and active older patients.

Methods and results
Patients aged 75 years or more with myocardial infarction (MI) and multivessel disease were randomized to receive physiology-guided complete revascularization or culprit-only strategy. The primary outcome was a composite of death, MI, stroke, or any revascularization within a year. Secondary endpoints included the composite of cardiovascular death or MI, as well as single components of the primary endpoint. Pre-admission physical activity was categorized into three groups: (i) absent (sedentary), (ii) light, and (iii) vigorous. Among 1445 patients, 692 (48%) were sedentary, whereas 560 (39%) and 193 (13%) performed light and vigorous physical activity, respectively. Patients engaging in light or vigorous pre-admission physical activity exhibited a reduced risk of the primary outcome compared with sedentary individuals [light hazard ratio (HR) 0.70, 95% confidence interval (CI) 0.55–0.91 and vigorous HR 0.14, 95% CI 0.07–0.91, respectively]. These trends were also observed for death, cardiovascular death, or MI. When comparing physiology-guided complete revascularization vs. culprit-only strategy, no significant interaction was observed for primary and secondary endpoints when stratified by sedentary or active status.

Conclusion
In older patients with MI, pre-admission physical activity emerges as a robust and independent prognostic determinant. Physiology-guided complete revascularization stands out an effective strategy in reducing ischaemic adverse events, irrespective of pre-admission physical activity status.

Clinical Trial Registration
ClinicalTrials.gov NCT03772743.
AI Originality Revisited: Can We Prompt Copyright over AI-Generated Pictures?
Authors: He Tianxiang 
Published: March 2024

Abstract
With the advent of large language models (LLMs) that can generate qualified content, such as images based on text prompts, the threat posed by AI creativity has become imminent. Courts and institutions in the US and China have provided different answers with regard to the copyrightability of prompt-based images. This article argues that the judicial recognition of text-to-image copyrightability at the current stage is dangerous. Not only is the practice not in accordance with our traditional understanding of originality and the author-work relationship; it also fails to consider the collateral consequences of such a finding. Perfectly regulating AI-generated content (AIGC) may be beyond the judiciary’s capacity, as the solutions are provided within an ill-suited framework. It would be preferable for legislators to engage in thorough discussions with stakeholders to develop a considered regulatory plan first, which does not necessarily have to revolve around copyright.
Dietary Interventions and Supplementation in Patients With an Ileal Pouch–Anal Anastomosis: A Systematic Review
Authors: Gold Stephanie , Levinson Carrie , Colombel Jean-Frederic , Manning Laura , Sands Bruce E , Kayal Maia 
Published: March 2024

Lay Summary
The restorative proctocolectomy with ileal pouch–anal anastomosis is the preferred surgery for patients with medically refractory ulcerative colitis. Although the ileal pouch–anal anastomosis provides gastrointestinal continuity and is an excellent alternative to a permanent end ileostomy, it is not without its complications including acute pouchitis, which occurs in up to 80% of patients. Diet may have a significant impact on pouch function and the development of pouchitis by virtue of its impact on motility and the microbiome. Multiple studies have evaluated the ability of different diets and supplements to improve pouch function and manage pouchitis, yet results are conflicting; thus, evidence-based dietary recommendations are lacking. Patients with an ileoanal pouch routinely ask about dietary interventions to maintain pouch health, and it is crucial that concrete evidence-based recommendations are identified to provide guidance. The goal of this systematic review is to summarize the available data on dietary patterns in patients with an ileoanal pouch, dietary interventions in this cohort, and the impact of supplements on pouch function and pouchitis.
Seasonal shift in energy allocation from somatic growth to lipid storage and the link between pre-winter condition and overwintering potential in juvenile Pacific cod
Authors: Abookire Alisa A , Copeman Louise A , Litzow Michael A , Laurel Benjamin J 
Published: March 2024

Abstract
Warming temperatures in the Gulf of Alaska have been linked to recruitment failure in Pacific cod (Gadus macrocephalus), but the mechanisms and timing of mortality events for juveniles are unclear. To date, limited research has focused on overwintering success, and the knowledge of juvenile ecology and physiology is based entirely on summer observations. Here, we investigate the changes in body condition, diet composition, and tissue-specific fatty acid (FA) storage for age-0 Pacific cod in Kodiak, Alaska, from February to December during 2018 and 2020. We observed protracted nearshore residency from June to December. Cod body condition (Kdry) and predicted weight at length were lowest in October, November, and December. Although not different interannually, diet composition varied seasonally, which corresponded to an increase in cod length. A range of condition metrics (HSIwet, FA concentration in liver tissue, and the % of whole body FAs stored in the liver) began to increase in September. Cod prioritized growth during the summer, while in the autumn and pre-winter they allocated more energy into lipid storage. We conclude that seasonal changes in tissue-specific FA storage and pre-winter fish conditions are important factors to consider for understanding overwintering potential of juvenile Pacific cod.
A short-term wind speed prediction method based on the BLS–RVM hybrid model
Authors: Geng Jianchun , Wen Lili 
Published: March 2024

Abstract
The study introduces a novel approach to short-term wind speed forecasting, which amalgamates statistical and machine learning techniques through the utilization of a hybrid model consisting of the broad learning system (BLS) and the relevance vector machine (RVM), to account for the nonstationary characteristics of wind speed data. Firstly, the initial wind speed time series is preprocessed using singular spectrum analysis to achieve noise reduction. Subsequently, the denoised wind speed time series is predicted using generalized learning system (BLS), and the prediction error is obtained. Finally, the prediction error of BLS is further predicted using RVM, and the final prediction result is obtained by combining it with the prediction results of BLS. By integrating multiple algorithms, this novel wind speed forecasting hybrid model improves prediction accuracy and can adapt to different wind speed characteristics and complex wind speed fluctuations. Through a case study, we find that this model outperforms other comparative models in terms of prediction, fully demonstrating its superiority.
Blunted Cortisol Response to Acute Psychosocial Stress in Women With Premenstrual Dysphoric Disorder
Authors: Hamidovic Ajna , Davis John , Soumare Fatimata 
Published: March 2024

Abstract

Background
Despite being considered a stress-related condition, it is not known whether the hypothalamic-pituitary-adrenal (HPA) axis is dysfunctional in response to acute psychosocial stress in premenstrual dysphoric disorder (PMDD). This is problematic because many women with PMDD report that they are not able to control their stress levels, and a blunted cortisol output has been identified in women with related psychiatric conditions, such as anxiety and depression. The present study is a part of the Premenstrual Hormonal and Affective State Evaluation (PHASE) project, and it aimed to characterize the cortisol trajectory in response to an acute psychosocial stress challenge.

Methods
Women with PMDD and healthy controls with confirmed ovulatory cycles underwent the Trier Social Stress Test (TSST) procedure in the mid-late luteal phase of the menstrual cycle, throughout which we collected serum samples of cortisol that we analyzed using ultra-performance liquid chromatography tandem mass spectrometry.

Results
The linear mixed model analysis indicated a significant time*diagnosis interaction (P = .008) such that women with PMDD displayed significantly lower serum cortisol levels at +40 through +90 minutes from the time of stress induction.

Conclusion
This is the first study to show that women with PMDD have a blunted cortisol response to psychosocial stress. Combined with our earlier finding showing a greater parasympathetic nervous system withdrawal on heart oscillations in PMDD during acute stress, these and other results show that the dysregulated processing of stress in PMDD may be captured using objective study measures.
Sporadic regional re-emergent cholera: a 19th century problem in the 21st century
Authors: Al-Adham Ibrahim S I , Jaber Nisrein , Ali Agha Ahmed S A , Al-Remawi Mayyas , Al-Akayleh Faisal , Al-Muhtaseb Najah , Collier Phillip J 
Published: March 2024

Abstract
Cholera, caused by Vibrio cholerae, is a severe diarrheal disease that necessitates prompt diagnosis and effective treatment. This review comprehensively examines various diagnostic methods, from traditional microscopy and culture to advanced nucleic acid testing like polymerase spiral reaction and rapid diagnostic tests, highlighting their advantages and limitations. Additionally, we explore evolving treatment strategies, with a focus on the challenges posed by antibiotic resistance due to the activation of the SOS response pathway in V. cholerae. We discuss promising alternative treatments, including low-pressure plasma sterilization, bacteriophages, and selenium nanoparticles. The paper emphasizes the importance of multidisciplinary approaches combining novel diagnostics and treatments in managing and preventing cholera, a persistent global health challenge. The current re-emergent 7th pandemic of cholera commenced in 1961 and shows no signs of abeyance. This is probably due to the changing genetic profile of V. cholerae concerning bacterial pathogenic toxins. Given this factor, we argue that the disease is effectively re-emergent, particularly in Eastern Mediterranean countries such as Lebanon, Syria, etc. This review considers the history of the current pandemic, the genetics of the causal agent, and current treatment regimes. In conclusion, cholera remains a significant global health challenge that requires prompt diagnosis and effective treatment. Understanding the history, genetics, and current treatments is crucial in effectively addressing this persistent and re-emergent disease.
Decentralized Targeting of Agricultural Credit Programs: Private Versus Political Intermediaries
Authors: Maitra Pushkar , Mitra Sandip , Mookherjee Dilip , Visaria Sujata 
Published: March 2024

Abstract
We conduct a field experiment in India comparing two ways of delegating selection of microcredit clients among smallholder farmers to local intermediaries: a private trader (TRAIL), versus a local–government appointee (GRAIL). Selected beneficiaries in both schemes were equally likely to take up and repay loans, and experienced similar increases in borrowing and farm output. However farm profits increased and unit costs of production decreased significantly only in TRAIL. While there is some evidence of superior selection by ability and landholding in TRAIL, the results are mainly driven by greater reduction of unit production costs for TRAIL treated farmers than GRAIL treated farmers of similar ability or landholding. We develop and test a model where the TRAIL agents’ role as middlemen in the agricultural supply chain enabled and motivated them to offer treated farmers business advice, which helped them lower unit costs.
Metabolic regulation of neutrophil functions in homeostasis and diseases
Authors: Leblanc Pier-Olivier , Bourgoin Sylvain G , Poubelle Patrice E , Tessier Philippe A , Pelletier Martin 
Published: March 2024

Abstract
Neutrophils are the most abundant leukocytes in humans and play a role in the innate immune response by being the first cells attracted to the site of infection. While early studies presented neutrophils as almost exclusively glycolytic cells, recent advances show that these cells use several metabolic pathways other than glycolysis, such as the pentose phosphate pathway, oxidative phosphorylation, fatty acid oxidation, and glutaminolysis, which they modulate to perform their functions. Metabolism shifts from fatty acid oxidation–mediated mitochondrial respiration in immature neutrophils to glycolysis in mature neutrophils. Tissue environments largely influence neutrophil metabolism according to nutrient sources, inflammatory mediators, and oxygen availability. Inhibition of metabolic pathways in neutrophils results in impairment of certain effector functions, such as NETosis, chemotaxis, degranulation, and reactive oxygen species generation. Alteration of these neutrophil functions is implicated in certain human diseases, such as antiphospholipid syndrome, coronavirus disease 2019, and bronchiectasis. Metabolic regulators such as AMPK, HIF-1α, mTOR, and Arf6 are linked to neutrophil metabolism and function and could potentially be targeted for the treatment of diseases associated with neutrophil dysfunction. This review details the effects of alterations in neutrophil metabolism on the effector functions of these cells.
Business, as usual? The impact of organized economic interests on the stringency of Covid-19 containment policies: insights from the Italian case
Authors: Tassinari Arianna , Panarello Demetrio , Tassinari Giorgio , Alboni Fabrizio , Drudi Ignazio , Bagnardi Francesco 
Published: March 2024

Abstract
How do organized economic interests affect the governance of the coronavirus disease 2019 (Covid-19) pandemic? We investigate whether the structural and instrumental power of employer organizations and unions impact upon the stringency of containment measures implemented by governing authorities to tackle the Covid-19 pandemic, focusing on Italy during the first Covid-19 wave of early 2020 as a crucial case. Using Hausman–Taylor panel regression models and original indicators of regional stringency and of unions and employers’ organizations’ efforts to exercise instrumental power via public pressure on social media, we find that the intensity of public pressure by employer organizations is negatively correlated with the stringency of the policy responses implemented by regional authorities to tackle the Covid-19 pandemic, whilst union pressures only show a limited effect. Our findings demonstrate that business pressure and the interplay of economic and class interests are constitutive of the governance of a crucial social and public health phenomenon such as the Covid-19 pandemic.
The adjuvant effect of oil-based gastric lavage on the outcome of acute Aluminum phosphide poisoning: a systematic review and meta-analysis
Authors: De Santi Omar , Orellana Marcelo J , Di Niro Cecilia A , Lashin Heba I , Greco Vanina 
Published: March 2024

Abstract

Introduction
Aluminum Phosphide (AlP) poisoning constituted the most common cause of poisoning death in some low- and middle-income countries (LMICs). This study aimed to evaluate the safety and efficacy of oil-based gastric lavage (GL) compared with standard therapy for the treatment of AlP poisoning. Materials and methods. This systematic review complied with “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) Protocols. A comprehensive search was carried out, identifying randomized controlled trials (RCTs), including anyone presenting within 6 h of exposure to AlP, and the administration of GL with oils, including liquid paraffin or coconut oil.

Results
We identified 7 RCTs. The evidence from 4 RCTs indicates that GL with paraffin oil is an effective treatment for acute AlP poisoning, decreasing the mortality rate (RR = 0.62; 95% CI = 0.48 to 0.81; participants = 226; I 2 = 10%; low-quality evidence). We estimate the Number Needed to Treat of 4. Likewise, this intervention reduces the need for intubation and mechanical ventilation (RR = 0.62; 95% CI = 0.40 to 0.79; I2 = 0%; low-quality evidence). Regarding GL with coconut oil, the evidence from 4 RCTs, indicates a slight reduction in mortality (RR = 0.82; 95% CI = 0.69 to 0.98; participants = 112; I2 = 0%; very low-quality evidence).

Conclusions
Limited evidence suggests that GL with paraffin oil is effective in reducing the mortality rate. Likewise, limited evidence showed in favor of paraffin oil concerning the need for intubation and mechanical ventilation. Very limited evidence suggests that GL with coconut oil could reduce mortality. Both interventions would have a benign safety profile.