bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasmodium falciparum‐specific human T cell clones: evidence for helper and cytotoxic activities
Authors: Sinigaglia Francesco , Matile Hugues , Richard J. , Pink L. 
Published: March 2007

AbstractThis report describes the isolation, and the phenotypic and functional characterization of Plasmodium falciparum‐specific human T lymphocyte clones (TLC) obtained from 2 acutely infected and 4 clinically immune donors. Approximately one third of the TLC obtained from the acutely infected patients had the phenotype CD8+/CD4−. No such clones were obtained from the clinically immune donors. P. falciparum‐specific, major histocompatibility complex‐restricted CD8+ clones can lyse unrelated tumor cell targets in the presence of anti‐CD3 antibodies, suggesting that these clones have cytotoxic potential. Conversely no killing was obtained with two CD4+ P. falciparum‐specific TLC, even in the presence of anti‐CD3 antibody. In addition the helper function of a CD4+ clone was demonstrated in a T/B cell cooperation system.
Plasmodium falciparum‐specific human T cell clones: recognition of different parasite antigens
Authors: Richard J. , Pink L. , Rijnbeek Anne‐Marie , Reber‐Liske Rosemaria , Sinigaglia Francesco 
Published: March 2007

AbstractT lymphocyte clones specific for malarial (Plasmodium falciparum) blood stage antigens were obtained from acutely infected patients or from donors living in a malaria‐endemic area of West Africa. Thirty‐four clones carrying the CD4 antigen, and one CD8+ clone, were tested in a proliferation assay for their capacity to recognize P. falciparum isolates of different geographical origins. Only one clone distinguished between different parasite isolates (it failed to react with a parasite isolate originating from East Africa, but did recognize West African and Asian isolates). All of the clones responded well to intact erythrocytes containing viable parasites, but some responded poorly to extracts of parasitized cells. Eight of 19 clones studied (all CD4+) recognized parasite antigens which had characteristic mobilities in sodium dodecyl sulfate‐containing polyacrylamide gels. The antigens had apparent molecular weights of about 20000, 35000, 40000, 120000, 150000–200000 and 200000. These results (together with a previous report of two clones recognizing an antigen of molecular weight about 50 000, Sinigaglia and Pink, EMBO J. 1985. 4: 3819) show that T cells in infected individuals react with at least 6 different parasite proteins.
Bone marrow pro‐T and pro‐B lymphocyte clones express functional receptors for interleukin (IL) 3 and IL4/BSF‐1 and nonfunctional receptors for IL2
Authors: Sideras Paschalis , Palacios Ronald 
Published: March 2007

AbstractIt is shown here that the C4‐77 and C4‐86 bone marrow clones with properties of pro‐T lymphocytes and the Bc/Bm 11 and CB/Bm7 clones with characteristics of pro‐B lymphocytes grow in recombinant interleukin 4 (rIL4)/BSF‐1 and IL3, but not in rIL2. The proliferative cell responses to rIL4/BSF‐1 were always less that ∼ 50% of those achieved by the clones in response to IL3. The CC11 monoclonal antibody (mAb) specific for IL3‐sensitive mouse cells did not affect the action of rIL4/BSF‐1, but it did inhibit the action of IL3 on the clones. The PC61 mAb against IL2 receptors had no effect on either rIL4/BSF‐1‐ or IL3‐driven responses. All four clones carry on the cell membrane the glycoproteins recognized by the CC11 mAb and by the PC61 mAb as assessed by immunofluorescence staining and flow cytometry. We conclude that the pro‐T and the pro‐B clones express functional receptors for IL3 and rIL4/BSF‐1 and nonfunctional receptors for IL2, that rIL4/BSF‐1 promotes growth of these clones via an IL3‐ and IL2‐independent pathway and discuss the possible biological significance of these findings.
Identification of interleukin 2‐producing T helper cells within murine Lyt‐2+ T lymphocytes: frequency, specificity and clonal segregation from Lyt‐2+ precursors of cytotoxic T lymphocytes
Authors: Heeg Klaus , Steeg Christiane , Hardt Conny , Wagner Hermann 
Published: March 2007

AbstractThe prime aim of this study was to assess whether the autonomous primary mixed lymphocyte culture response of Lyt‐2+ T cells towards class I major histocompatibility complex (MHC) antigens reflects in terms of interleukin 2 (IL2) production and cytotoxicity the activation of multifunctional Lyt‐2+ T cells, or the activation of functionally distinct T cell subsets. The results demonstrate that highly purified Lyt‐2+ T cells proliferate in response to class I MHC antigens, as opposed to L3T4+ T cells which react towards class II MHC antigens. In both responder cell types proliferative responses are associated with IL2 secretion, while only Lyt‐2+ T cells develop measurable cytotoxic effector cells. The precursor frequency of IL 2‐producing helper cells in MHC class I‐reactive Lyt‐2+ T cells equals that in MHC class II‐reactive L3T4+ T cells (f = 1/500–1/1000). In clonal segregation analysis > 90% of Lyt‐2+ colonies secreting IL2 do not develop cytotoxic activity, while > 90% of Lyt‐2+ cytotoxic T cells fail to produce detectable IL2. A minority of < 10% of Lyt‐2+ T cells appears to be bifunctional. As such the results point out the existence of functionally committed T cells within class I MHC‐reactive Lyt‐2+ T cells able to produce either IL2 or to develop into cytotoxic effector cells.
Isogeneic monoclonal antibodies against anti‐α(1 → 3)dextran idiotypes II. Neonatally induced idiotope‐specific suppression: a comparative analysis
Authors: Wilke Joachim , Lehle Georg , Weiler Eberhardt 
Published: March 2007

AbstractFrom a panel of isogeneic monoclonal anti‐idiotope antibodies several were used as agents in neonatal idiotope suppression. They differed from one another in isotype, and in idiotope specificity, as described in the preceding report (Eur. J. Immunol. 1987. 17: 255). In their effects they were compared with respect to the following variables: (a) minimum dose required for suppression; (b) duration of suppression, and its relationship to the dose applied neonatally; (c) half‐life of anti‐idiotope in the immune system of the young mice; (d) specificity of suppression as achieved by a given anti‐idiotope: in how far does it affect idiotopes defined by alternate anti‐idiotopes? The following results were obtained: (a) the minimum effective dose varied widely between anti‐idiotopes. One, belonging to the IgM class, was completely ineffective; others varied from ≈ 10 μg/mouse, required for complete suppression, to ≈ 100 μg/mouse. (b) The dose‐response characteristic was independent of whether the state of suppression was tested (by immunization against α(1 → 3)dextran) 26 days or 70 days after neonatal anti‐idiotope treatment. We take this as an indication that the anti‐idiotope effect occurs during an early postnatal period. (c) There appeared to be a relationship between the rate of decay of anti‐idiotope in the system and the dose required for complete suppression: the faster the decay, the more is needed initially. The persistence of effective molecules in the animals appears to depend on their isotype (as has been noted by others before): IgM decays fastest, and was ineffective in our experiments; IgG1 stays longest, and the smallest dose was required for suppression. IgG2b was intermediate. (d) The specificity of neonatal suppression was clearly correlated with the serological specificity of the anti‐idiotope monoclonal antibodies, as well as with the representation of the corresponding idiotopes in physiological anti‐dextran sera, as described in the preceding report: private anti‐idiotopes suppressed their counterpart idiotopes only, while the public anti‐idiotope suppressed all other idiotopes in concert.
Stimulatory effects of cyclosporin A on human and mouse thymic epithelial cells
Authors: Dardenne Mireille , Savino Wilson , Feutren Gilles , Bach Jean‐François 
Published: March 2007

AbstractWhen injected daily into normal young or aging mice, cyclosporin A (CsA) induces a significant stimulation of thymic hormone (thymulin) secretion, as measured by the peripheral level of the hormone and the number of thymulin‐producing cells in the thymus. This stimulation is dose dependent and reversible after the end of treatment. Similar findings have been made in primary cultures of human thymic epithelial cells in which CsA increases the percentage of thymulin‐producing cells evaluated by immunofluorescence, as well as the amount of hormone released into the supernatants. No effect of CsA is observed on the expression of class II major histocompatibility complex antigens. CsA also increases the proliferation of cultured human thymic epithelial cells, as assessed by bromodeoxyuridine and [3H]thymidine incorporation. The present study strongly suggests that CsA can stimulate the proliferation and endocrine function of thymic epithelial cells. Its action on the immune system is thus not restricted to lymphoid cells and might be partially mediated by its effect on the thymic epithelium.
Sialylation patterns of lymphocyte function‐associated antigen 1 (LFA‐1) differ between T and B lymphocytes
Authors: Takeda Akiko 
Published: March 2007

AbstractLymphocyte function‐associated antigen 1 (LFA‐1) was immunoprecipitated from various types of surface‐radioiodinated murine lymphocytes, and analyzed by two‐dimensional polyacrylamide gel electrophoresis. LFA‐1 α and β chains from splenic B lymphocytes had the same apparent molecular weights as, but distinct isoelectrofocusing patterns from, their counterparts from thymocytes or splenic T lymphocytes. The splenic B lymphocytes lacked a basically charged population of a chain, while the thymocytes and the splenic T lymphocytes showed both the acidic and the basic portions. Furthermore, the β chain of the former migrated more towards the acidic end than that of the latter. No difference was found between LFA‐1 molecules of the same lineage of cells from several strains of mice whose H‐2 haplotypes were different from one another. When murine lymphocyte lines were examined, LFA‐1 with various isoelectrofocusing patterns were recognized. The charge difference again reflected the difference in lymphocyte lineage, but in a more exaggerated manner than that seen with cells from mice. The average acidity of both chains of LFA‐1 decreased in the order of B cell lines, pre‐B cell lines and T cell lines. The lineage‐dependent charge difference of either chain disappeared after neuraminidase treatment of LFA‐1, indicating that lymphocyte differentation was accompanied by changes in LFA‐1 sialylation.
A new epitope of the T200 molecule family defined by the 3A35 monoclonal antibody and expressed by macrophages and activated T lymphocytes
Authors: Le Corre Rozenn , Gerlier Denis , Martin Anne , Le Garrec Yvonne , Corradin Giampetro , Bron Claude , Toujas Louis 
Published: March 2007

AbstractA monoclonal antibody (mAb), 3A35, produced against mouse macrophages (MΦ) was found to react against certain activated T cells. This mAb, a rat IgM, resulted from a cell fusion between a mouse plasmacytoma and rat lymphocytes immunized against mouse MΦ. It bound more avidly to activated than to resident MΦ. It did not react against B cells and resting T lymphocytes but recognized certain dividing T cells like EL4 lymphoma, concanavalin A‐activated and interleukin 2‐expanded spleen cells, and helper T cell hybridomas. By contrast, other T lymphocyte‐derived cell lines such as YAC‐1 and CTLL2 were unreactive. No clear relationship was found between the binding of 3A35 to cells and the expression of L3T4 and Lyt‐2 antigens. The specific stimulation of T cell clones with antigen rapidly induced a strong reactivity with 3A35 mAb which declined thereafter to a low (helper clones) or non‐reactivity (cytotoxic clones) after 10 days of culture. Immunoprecipitation experiments, performed with MΦ derived from bone marrow cell cultures, surface iodinated with 125I or metabolically labeled with [35S]methionine, showed that 3A35 bound to a 200‐kDa molecule, shifting to 175 kDa under reducing conditions. In peritoneal MΦ activated in vivo, in addition to the 175‐kDa band, new bands migrating at 140, 120 and 85 kDa were identified by 3A35 and could be absorbed on a commercial anti‐T200 mAb bound to Sepharose beads. After strengthening the cell binding of 3A35 to EL4 lymphoma cells by a cross‐linking agent, only a 85‐kDa molecule was immuno‐precipitated. Thus, 3A35 identifies a new epitope of the T200 molecule family which is expressed on MΦ and activated T cells.
Interactions between hepatitis B virus and polymeric human albumin I. Production of monoclonal anti‐idiotypes (anti‐anti‐polymeric human albumin) which recognize hepatitis B virus surface antigen
Authors: Colucci Giuseppe , Waksal Samuel D. 
Published: March 2007

AbstractIn an attempt to characterize the polymeric human albumin (polyHSA) receptor expressed on hepatitis B virus and hepatocytes, we have used a human anti‐polyHSA IgG to generate monoclonal anti‐idiotypes (anti‐Id) which bear the internal image of polyHSA and mimic its binding activity. Two monoclonal anti‐Id antibodies, 63.14 and 70.F9, were strongly reactive in both radioimmunoassay and enzyme‐linked immunosorbent assay (ELISA) with the F(ab′)2of the immunogen as well as with purified hepatitis B surface antigen (HBsAg) expressing various subtypes. The specificity of the binding of anti‐Id to HBsAg was confirmed in direct ELISA and by Western blot analysis. These experiments also showed that the anti‐Id bind to a site expressed on the major 24‐kDa protein of HBsAg particles, and that this recognition is specifically inhibited by polyHSA.Experiments on cellular staining and radioimmunoprecipitation on HBsAg‐positive and ‐negative cell lines showed that the anti‐Id recognize intracellular HBsAg but not other liver cell proteins, including the putative polyHSA receptor. These data indicate, therefore, that the monoclonal anti‐Id mimic the binding activity of polyHSA and recognize its binding site on the virus. The inability of both anti‐Id to react with the hepatocyte surface suggests either the absence of a specific hepatic polyHSA receptor or the expression of one with a different configuration.
Interactions between hepatitis B virus and polymeric human serum albumin II. Development of syngeneic monoclonal anti‐anti‐idiotypes which mimic hepatitis B surface antigen in the induction of immune responsiveness
Authors: Colucci Giuseppe , Beazer Yasmin , Waksal Samuel D. 
Published: March 2007

AbstractWe used monoclonal anti‐idiotypes (anti‐Id) 63.14, previously shown to mimic polymeric human serum albumin (polyHSA) and bind its receptor on hepatitis B surface antigen (HBsAg), to produce syngeneic monoclonal anti‐anti‐Id (Ab3) which could bear the internal image of HBsAg and mimic its immunogenicity in vivo. Nine hybridomas obtained from spleen cells of BALB/c mice immunized with 63.14 were isolated, which were able to inhibit the binding of alkaline phosphatase‐conjugated 63.14 to HBsAg. Both direct and competition enzyme‐linked immunosorbent assay (ELISA) showed that 4 of these clones were able to mimic HBsAg since they reacted with polyHSA and inhibited the binding of monoclonal and polyclonal anti‐HBsAg to the viral antigen. To determine whether these Ab3 could induce an immune response against HBsAg in vivo, we injected a series of rabbits with Ab3 G11 or HBsAg and tested their sera after the second boost. ELISA, radioimmunoassay and Western blot experiments showed that G11 was as effective as HBsAg in inducing a specific anti‐HBsAg immune response. These data indicate that our Ab3 can mimic HBsAg both in vitro and in vivo and might be useful as alternative vaccine for HBV infection.
Targeting cytotoxic T cells to antigen‐specific B lymphocytes
Authors: Rammensee Hans‐Georg , Julius Michael H. , Nemazee David , Langhorne Jean , Lamers Rinus , Köhler Georges 
Published: March 2007

AbstractA recent development in immunomanipulation involves the targeting of cytotoxic T lymphocytes (CTL) to cell‐bound antigens using bispecific antibodies. These antibodies have been engineered such that specificity is directed against the T cell receptor (TCR) or TCR‐associated T3 molecules, as well as against the chosen antigen. The present study was aimed to force interactions between T and B cells by bridging their receptors. F23.1 antibodies, which are specific for gene products of the TCR Vβ8 gene family, were conjugated with TNP (2,4,6‐trinitrophenyl) and this construct was used to bridge the receptors of Vβ8+ T cells with the receptors of TNP‐specific B cells. The bridging was demonstrated by direct killing of both a TNP‐specific B hybridoma and of blast cells from mice transgenic for μ,x of the TNP‐specific antibody Sp6. Further, F23.1‐TNP constructs in conjunction with Vβ8+ CTL were shown to specifically deplete Ig‐secreting B cells from Sp6 transgenic mice. Conjugates of TCR‐specific antibodies and antigen are theoretically useful in vivo to either deplete or expand B cells of a given specificity by coupling their receptors to the TCR of CTL or T helper cells, respectively.
Mouse fibroblasts that express a cloned gene for the human transferrin receptor induce a specific cytolytic T cell response
Authors: Maryanski Janet L. , Kühn Lukas C. , Corradin Giampietro 
Published: March 2007

AbstractFrom CBA mice immunized with mouse L cells that express a cloned gene for the human transferrin receptor (hTR) we have isolated cytolytic T cells (CTL). The CTL were Lyt‐2+/L3T4− and lysed L cell transfectant clones that expressed the hTR gene but not control untransfected L cells or transfectants selected for the expression of an unrelated human cell surface antigen, 4F2. Lysis of independent hTR transfectant clones correlated with the relative level of receptor expression. The availability of CTL directed against a non‐major histocompatibility complex‐controlled cellular protein‐like hTR may provide an interesting model system for studying antigen processing and recognition.
Comparison of inhibitory effect of galactose analogs on the binding and cytotoxicity of an anti‐globotriaosylceramide monoclonal antibody coupled or not coupled to pokeweed antiviral protein
Authors: Junqua Simone , Wils Pierre , Mishal Zohar , Le Pecq Jean‐Bernard 
Published: March 2007

AbstractThe results described here provided an example of a human IgM monoclonal antibody against a tumor‐associated glycolipid and of the unusual properties of its corresponding immunotoxin (IT). The monoclonal antibody referred to as 38‐13 has been previously described and reacted with the globotriaosylceramide [Gb3: Gal(α1→4)‐Gal(β1→4)‐Glc(β1→1)ceramide] specifically expressed on surface membrane of Burkitt's lymphoma (BL) cells. An immunotoxin (38‐13 IT) combined with the pokeweed antiviral protein (PAP) toxin via S‐S bridges showed paradoxically a lower cytotoxic effect in BL Ramos cells than in non‐BL cells such as leukemic mouse L1210 cells, while these cells appeared not to be involved by flow cytometric analysis and complement‐dependent cytotoxicity. Consequently, the inhibitory effect of selective galactose analogs on the binding and the cytotoxicity of 38‐13 antibody conjugated or not to PAP toxin was compared on BL and non‐BL cells. Only the galactose blocked in α configuration provided a fine inhibition of 38‐13 binding on BL Ramos cells and both α and β‐galactose allowed us to establish a clear distinction between the pathway entry of 38‐13 IT in BL and non‐BL cells; (a) in close correlation with the 38‐13 binding specificity the 38‐13 IT cytotoxic effect in Ramos BL cells could also be prevented by α‐Gal only, suggesting that this toxic action is probably mediated through the IT binding to Gb3 antigenic sites. (b) In contrast, on apparently irrelevant L1210 cells, 38‐13 IT showed a cytotoxic effect which was inhibited preferentially by lactose (Gal in β configuration). It was discussed that IT binding alone to either antigenic sites which are inhibited by the hapten α‐Gal, or nonspecific sites which can compete with the hapten β‐Gal is unable to induce efficient killing of cells. But cooperation of both bindings might give an attractive explanation of IT cytotoxic effect. It was concluded that the unexpected activity of 38‐13 IT in non‐BL cells probably could be mediated through an active macromolecular transport process which could implicate a β‐galactoside‐binding protein (lectin).
Provision of the immunoglobulin heavy chain enhancer downstream of a test gene is sufficient to confer lymphoid‐specific expression in transgenic mice
Authors: Reik Wolf , Williams Gareth , Barton Sheila , Norris Mike , Neuberger Michael , Surani M. Azim 
Published: March 2007

AbstractTo test whether the immunoglobulin heavy chain enhancer is sufficient to direct lymphoid specific gene expression in vivo, we have incorporated into the mouse germ line a test gene (chloramphenicol‐acetyl transferase) whose transcription is potentiated by the enhancer. Analysis of transgenic mice bearing single‐copy integrations of the injected DNA indicates that provision of the enhancer downstream of the test gene (beyond the polyadenylation site) is sufficient to direct lymphoid‐specific expression. The tissue specificity is considerable as judged by the fact that transgene expression is at least one thousandfold higher in spleen than in liver. Within the lymphoid family, expression of the transgene is much higher in surface IgM+ spleen cells than in thymocytes high in Thy‐1 expression, suggesting that the enhancer preferentially directs expression to cells of the B lineage.
Immune recognition of human major histocompatibility antigens: localization by a comprehensive synthetic strategy of the continuous antigenic sites in the first domain of HLA‐DR2 β chain
Authors: Ulrich Robert G. , Atassi Hammad , Lutz Paula , Cresswell Peter , Atassi M. Zouhair 
Published: March 2007

AbstractA comprehensive synthetic approach, previously developed in this laboratory, has been applied to screen the entire first domain (residues 1–95) of the HLA‐DR2 β chain for the full profile of continuous regions that are recognized by human alloantisera and anti‐DR or anti‐β chain antisera raised in other species. Nine consecutive peptides, that were 15 residues each and overlapped by 5 residues, covering the first domain of the DR2 β chain were synthesized, purified and characterized. The antibody‐binding activities of the peptides were determined by radioimmunosorbent titrations. This established the full profile of peptides having specific antibody‐binding activity with these various antisera. Three continuous antigenic sites were localized in this domain by all of antisera tested. Peptide 81–95 appeared to be the most immunodominant (i.e. bound the highest amounts of antibodies) in most antisera tested. Although their immunodominance varied with the antisera and boundary shifts were present, the submolecular regions recognized on the first domain of DR2β appeard to be similar with antisera against the DR2 (αβ1) complex or against the isolated β chain. Furthermore, recognition was independent of the host species from which the antisera were obtained.
Expression of the bcl‐2 gene in mouse B lymphocytic cell lines is differentiation stage specific
Authors: Gurfinkel Naama , Unger Tamar , Givol David , Mushinski J. Frederic 
Published: March 2007

AbstractcDNA clones of mouse bcl‐2 have been isolated and characterized by homology to the human bcl‐2 gene, a putative oncogene that is found on the portion of chromosome 18 characteristically involved in the t(14;18) translocation present in nearly all human follicular B cell lymphomas. Our mouse cDNA clone detects 7.9 and 6.3‐kb bcl‐2 RNAs in mouse B cell lymphomas, but only in tumors consisting of pre‐B and follicular center mature B cells, not in pro‐B cell or plasma cell tumors. Thus, this gene appears to be a B cell differentiation marker that is expressed only in committed B cells, but is shut off in end stage plasma cells. This pattern of expression is unique among oncogenes, and we suggest that it may be responsible for the high frequency of translocations at this locus in the common malignancy of human mature B cells, follicular lymphomas.