bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Paracoccus sp. EGY7 carotenoids on triple-negative breast cancer cells: invitro study
Authors: Abdelazim Karim , Hussein Ahmed , Khattab Sherine N. , El Feky Shaymaa Essam , Noby Nehad 
Published: February 2025

Abstract
This study investigates the effect of Paracoccus sp. EGY7 carotenoids on the triple-negative breast cancer cell line (MDA-MB-231). The bacterial strain was isolated, and its carotenoids profile was analyzed via HPLC-DAD-MS. Cytotoxicity, migration tests and the expression of BAX and BCL-2 at the gene and protein levels were carried out to evaluate the therapeutic efficiency of the produced carotenoids. Molecular docking analysis estimated the binding affinity between zeaxanthin and BCL-2 protein. Chromatographic analysis revealed zeaxanthin as the major carotenoid (48.3%). The extract exhibited significant cytotoxicity against MDA-MB-231 cells with an IC50 of 1200 µg. It notably reduced cell migration, with wound closure percentages of 37.50% and 79.17% for the 600 µg group, while the percentages were 12.50% and 53.50% for the 1200 µg group, compared to 71.67% and 95.67% for the control at 24 and 48 h post-treatment, respectively. The extract induced apoptosis, as evidenced by significantly increased BAX/BCL-2 gene expression ratios at 600 and 1200 µg (p < 0.05). Western blotting showed increased BAX protein expression at 600 and 1200 µg compared to the control group (p < 0.001), and significantly lower BCL-2 protein expression (p = 0.000005 for 1200 µg and p = 0.0001 for 600 µg). Docking analysis indicated a strong affinity of zeaxanthin to BCL-2 (ΔG = -9.773241 kcal/mol) compared to obatoclax (ΔG = -7.419345 kcal/mol). Paracoccus sp. EGY7 carotenoids are a promising anticancer agent against MDA-MB-231 cells. They effectively promote apoptosis and prevent metastasis, crucial for disease advancement in cancer cells.
Investigating the implementation of infection prevention and control practices in neonatal care across country income levels: a systematic review
Authors: Nyantakyi Emanuela , Baenziger Julia , Caci Laura , Blum Kathrin , Wolfensberger Aline , Dramowski Angela , Albers Bianca , Castro Marta , Schultes Marie-Therese , Clack Lauren 
Published: February 2025

Abstract

Background
Despite the proven effectiveness of infection prevention and control (IPC) practices in reducing healthcare-associated infections and related costs, their implementation poses a challenge in neonatal care settings across high-income (HICs) and low- and middle-income countries (LMICs). While existing research has predominantly focused on assessing the clinical effectiveness of these practices in neonatal care, aspects concerning their implementation remain underexplored. This systematic review therefore aimed to analyze implementation determinants and employed strategies for implementing IPC practices in inpatient neonatal care across country income levels.

Methods
Following a targeted search in seven databases, titles and abstracts as well as full texts were screened in a dual review process to identify studies focusing on the implementation of IPC practices in inpatient neonatal care and reporting on implementation determinants and/or implementation strategies. Implementation determinants were synthesized using the updated Consolidated Framework for Implementation Research. Implementation strategies were coded according to the Expert Recommendations for Implementing Change taxonomy. A convergent integrated approach was used to narratively summarize results across qualitative and quantitative studies. χ

2
Tests and Fisher’s Exact Tests were performed to analyze differences in implementation determinants and strategies across IPC practices and country income levels. The quality of included studies was assessed using the Mixed Methods Appraisal Tool.

Results
Out of 6,426 records, a total of 156 studies were included in the systematic review. Neonatal units in LMICs and HICs showed general commonalities in reported implementation determinants, which were mainly reported at the organizational level. While educational as well as evaluative and iterative strategies were most frequently employed to support the implementation of IPC practices in both LMICs and HICs, other strategies employed showed variance across country income levels. Notably, the statistical analyses identified a significant association between country income levels and implementation determinants and strategies respectively (
$$\:\rho\:$$
<0.05).

Conclusion
The results of this systematic review underscore the importance of the organizational level for the implementation of IPC practices in neonatal care irrespective of country income level. However, further research is needed to understand the underlying relationships of factors and dynamics contributing to the observed practice variances in LMICs and HICs.

Registration
PROSPERO (CRD42022380379).
The mechanical test as a supplemental decision support tool for the safe removal of an Ilizarov circular external fixator
Authors: Xu Yulin , Liu Jialin , Yang Jinghong , Zhang Tao , Li Zhong , Liu Yanshi 
Published: February 2025

Abstract

Background
Timing the fixator removal is vital for a successful external fixation treatment. The purpose of this study was to determine the effectiveness of axial load–share ratio in vivo as a supplemental decision support tool for the safe removal of an Ilizarov circular external fixator.

Methods
This prospective observational study consists of 83 patients undergoing tibial or femoral lengthening with Ilizarov circular external fixation in our institution, from January 2011 to October 2019. In group I (38 patients), the external fixator was removed based on the surgeon’s clinical experience and radiographs from January 2011 to June 2015. In group II (45 patients), from July 2015 to October 2019, the supplemental axial load–share (LS) ratio test was accomplished without the knowledge of the clinical results by another medical team. The test was performed by electronically measuring forces in the fixator rods and in a ground force plate. When the LS ratio < 10% was consistent with the conclusion (dense bone formation was achieved in the distraction zone) drawn from the corresponding routine radiographs by the treating surgeon, the external fixator was removed.

Results
There was no statistical significance in demographic data between the two groups (P > 0.05). In group I, 4 of the 38 patients suffered refracture (the refracture rate was 10.5%) after fixator removal, and bone union was finally achieved with further intervention by intramedullary nail. In group II, 36 patients terminated the external fixation after the first mechanical test, and another 9 patients terminated the external fixation at the subsequent test. None of the 45 patients in group II suffered refracture (the refracture rate was 0%). There was statistical significance in the refracture rate between the two groups (P < 0.05).

Conclusions
Adequate assessment of bone regenerate is crucial before removing an external fixator to prevent deformation or refracture. The axial load–share ratio in vivo is a practically quantitative method to supplement radiography and clinical experience for the assessment of regenerate healing, and the axial load–share ratio dropped below 10% is a safe limit for the Ilizarov circular external fixator removal.
Antibiotic-associated changes in Akkermansia muciniphila alter its effects on host metabolic health
Authors: Han Yumin , Teng Teh Min , Han Juwon , Kim Heenam Stanley 
Published: February 2025

Abstract

Background
Altered gut microbiota has emerged as a major contributing factor to the etiology of chronic conditions in humans. Antibiotic exposure, historically dating back to the mass production of penicillin in the early 1940s, has been proposed as a primary contributor to the cumulative alteration of microbiota over generations. However, the mechanistic link between the antibiotics-altered microbiota and chronic conditions remains unclear.

Results
In this study, we discovered that variants of the key beneficial gut microbe, Akkermansia muciniphila, were selected upon exposure to penicillin. These variants had mutations in the promoter of a TEM-type β-lactamase gene or pur genes encoding the de novo purine biosynthesis pathway, and they exhibited compromised abilities to mitigate host obesity in a murine model. Notably, variants of A. muciniphila are prevalent in the human microbiome worldwide.

Conclusions
These findings highlight a previously unknown mechanism through which antibiotics influence host health by affecting the beneficial capacities of the key gut microbes. Furthermore, the global prevalence of A. muciniphila variants raises the possibility that these variants contribute to global epidemics of chronic conditions, warranting further investigations in human populations.
Bacterial community assembly of specific pathogen-free neonatal mice
Authors: Kennedy Elizabeth A. , Weagley James S. , Kim Andrew H. , Antia Avan , DeVeaux Anna L. , Baldridge Megan T. 
Published: February 2025

Abstract

Background
Neonatal mice are frequently used to model diseases that affect human infants. Microbial community composition has been shown to impact disease progression in these models. Despite this, the maturation of the early-life murine microbiome has not been well-characterized. We address this gap by characterizing the assembly of the bacterial microbiota of C57BL/6 and BALB/c litters from birth to adulthood across multiple independent litters.

Results
The fecal microbiome of young pups is dominated by only a few pioneering bacterial taxa. These taxa are present at low levels in the microbiota of multiple maternal body sites, precluding a clear identification of maternal source. The pup microbiota begins diversifying after 14 days, coinciding with the beginning of coprophagy and the consumption of solid foods. Pup stool bacterial community composition and diversity are not significantly different from dams from day 21 onwards. Short-read shotgun sequencing-based metagenomic profiling of young pups enabled the assembly of metagenome-assembled genomes for strain-level analysis of these pioneer Ligilactobacillus, Streptococcus, and Proteus species.

Conclusions
Assembly of the murine microbiome occurs over the first weeks of postnatal life and is largely complete by day 21. This detailed view of bacterial community development across multiple commonly employed mouse strains informs experimental design, allowing researchers to better target interventions before, during, or after the maturation of the bacterial microbiota. The source of pioneer bacterial strains appears heterogeneous, as the most abundant taxa identified in young pup stool were found at low levels across multiple maternal body sites, suggesting diverse routes for seeding of the murine microbiome.
Fabrication of Au-doped mesoporous TiO2 supported on g-C3N4 as an efficient light-assisted catalyst for oxidative desulfurization of model fuels with different sulfur content
Authors: Moeinifard Behzad , Najafi Chermahini Alireza 
Published: February 2025

Abstract
In the present study, the mesoporous TiO2 that was supported on graphitic carbon nitride (g-C3N4) was decorated with Au. The nanocomposite (Au-m-TiO2/g-C3N4) has been successfully synthesized by combining sono-chemical and EISA methods and was subsequently used in photocatalytic oxidative desulfurization of dibenzothiophene (DBT) in n-octane as a model fuel and under visible illumination. The Au-m-TiO2/g-C3N4 photocatalyst was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), UV–Vis diffusive reflectance spectra (UV–Vis DRS), high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared spectra (FT-IR), and nitrogen adsorption measurements. The removal efficiency of DBT was 98.7% under the optimized reaction conditions and under visible-light irradiation (λ > 400 nm).
Nutritional knowledge and practices of low-income women during pregnancy: a qualitative study in two Oaxacan cities
Authors: Marian Marian , Pérez Ramona L. , McClain Amanda C. , Hurst Samantha , Reed Elizabeth , Barker Kathryn M. , Lundgren Rebecka 
Published: February 2025

Abstract

Background
Adequate dietary intake is critical for healthy pregnancies. Recent changes in social services in Mexico, coupled with high levels of food insecurity, call into question whether expecting women of the lowest socioeconomic status are able to meet their dietary and nutritional needs in this changing context. The aim of this study was to explore the nutritional practices, education and received and employed among women during their pregnancy.

Methods
Guided by Ecological Systems Theory and an Intersectionality Framework, this qualitative study was carried out in Oaxaca City and Puerto Escondido, in Oaxaca, a Mexican state with high levels of food insecurity. Women who had at least one child in the past five years and had lived in Oaxaca for the past five years were eligible to participate. Twenty-five women participated in semi-structured in-depth interviews conducted between June and December of 2023. A grounded theory approach was used for coding. NVivo was used for coding and analyses.

Results
Five key themes emerged linked to individual-level characteristics and the multiple social identities related to the social support for nutritional knowledge and practices among low-income Oaxacan women during pregnancy: (1) Life experiences, sociodemographic, and health characteristics that influence nutritional practices and knowledge during pregnancy; (2) Female family members as a primary source of nutritional knowledge and food support; (3) Support from other members of women’s social networks; (4) Medical guidance for nutrition during pregnancy; and (5) Quality and gaps in the broader health care system and social services. These themes highlight how women’s own experiences and social identities and the different interpersonal and community-level environments, particularly those of mothers and grandmothers and health care providers, interact and shape women’s nutritional knowledge and practices, such as foods and nutritional supplements consumed, during pregnancy.

Conclusion
Nutritional knowledge and practices during pregnancy are impacted by multiple social identities women have and different factors at the individual, group, and structural level. Future multi-level approaches in research and programming that take into consideration the individual, family members, and other social influences are needed to address the gaps in nutrition that women in Oaxaca go through during the prenatal period.
Proximity induced superconductivity and Andreev reflection on the surface of nodal Weyl semimetals
Authors: Hu Xiang 
Published: February 2025

Abstract
In nodel Dirac semimetals the bulk conduction and valence bands touch along a line, typically a ring. One of the key properties of nodal Dirac semimetals is to have drumhead surface states, i.e. surface states with a very large effective mass. In the presence of certain types of spin-rotation symmetry breaking perturbations the Dirac ring split into two Weyl rings. In this work, we study the superconducting pairing induced via the proximity effect to the surface of nodal Weyl semimetals. We find how the the tunneling to the surface affects the symmetry of the proximity-induced superconducting pairing. We then calculate the Andreev reflection and show that it can be used to probe the drumhead states.
Prevalence and Determinants of Diabetes Mellitus in 2338 Long-Term Dutch Childhood Cancer Survivors (DCCS-LATER2 Study)
Authors: Bolier Melissa , de Winter Demi T C , Fiocco Marta , van den Berg Sjoerd A A , Bresters Dorine , van Dulmen-den Broeder Eline , van der Heiden-van der Loo Margriet , Hoefer Imo , Janssens Geert O , Kremer Leontien C M , Loonen Jacqueline J , Louwerens Marloes , van der Pal Heleen J , Pluijm Saskia M F , Tissing Wim J E , van Santen Hanneke M , de Vries Andrica C H , van der Lely Aart-Jan , van den Heuvel-Eibrink Marry M , Neggers Sebastian J C M M 
Published: February 2025

Abstract

Context
Diabetes mellitus (DM), a possible adverse effect of childhood cancer treatment, is strongly associated with cardiovascular disease and early mortality in adult childhood cancer survivors (CCS).

Objective
Here, we assess the prevalence and determinants of DM in our nationwide CCS cohort.

Methods
In this cross-sectional study, the prevalence of DM was assessed in 2338 CCS, using the Lifelines cohort (n = 132 226 adults with no history of cancer) as a reference. DM was defined through serum glucose measurement (fasting glucose ≥7.0 mmol/L or nonfasting ≥11.1 mmol/L) and/or self-report (previous diagnosis and/or medication use). Multivariable logistic regression models, adjusted for age, sex, and body mass index (BMI), were used to assess the cohort effect on the presence of DM. Multivariable logistic regression analysis was used to identify determinants of DM in CCS.

Results
Survivors (median age 34.7 years, median follow-up time 27.1 years) showed increased odds for hyperglycemia (aOR = 2.72; 95% CI, 2.06-3.59), previous DM diagnosis (aOR = 3.03; 95% CI, 2.33-3.95), and antidiabetic medication use (aOR = 2.94; 95% CI, 2.17-3.99), compared to the reference cohort. Age (OR = 4.32; 95% CI, 1.84-10.15, &gt;35 vs 18-35 years), BMI (OR = 1.12; 95% CI, 1.08-1.16, per point), family history of DM (OR = 2.38; 95% CI, 1.51-3.76), prior abdominal/pelvic radiotherapy (OR = 4.19; 95% CI, 2.32-7.55), total body irradiation (OR = 14.31; 95% CI, 6.98-29.34), hypogonadism (OR = 2.40; 95% CI, 1.15-4.99), hypertension (OR = 1.71; 95% CI, 1.06-2.76), and dyslipidemia (OR = 3.81; 95% CI, 2.15-6.75) were associated with DM in CCS. A statistically significant interaction between age and sex on the development of DM in survivors was identified.

Conclusion
The identified 3-fold increased risk of DM in CCS, along with the clinically relevant and some modifiable determinants, underscores the importance of early risk-based screening and the exploration of lifestyle interventions in this population.
Streptococcus pneumoniae meningitis complicated by hygroma and vasculitis
Authors: Dib Abdelkader , Salem Johny , Dawood Mohamad , Osman Omar , Fares Mirna 
Published: February 2025

Hygroma and vasculitis are rare complications of meningitis, that are serious and potentially fatal. In this case report, we present a 71-year-old male diagnosed with Streptococcus pneumoniae meningitis who developed bilateral subdural hygroma and vasculitis despite appropriate treatment in the intensive care unit. The patient experienced seizures and persistent febrile episodes, leading to the diagnosis of these complications. Despite surgical and medical management, the patient’s neurological status did not improve, and he remained comatose and was eventually transitioned to palliative care.
Challenging a paradigm: Staggered versus single-pulse mass dog vaccination strategy for rabies elimination
Authors: Bellotti Brinkley Raynor , Díaz Elvis W. , De la Puente-León Micaela , Rieders Maria T. , Recuenco Sergio E. , Levy Michael Z. , Castillo-Neyra Ricardo 
Published: February 2025

Background
From smallpox to poliomyelitis, halting contagion transmission through simultaneous mass vaccination is ubiquitous and often perceived as the only possible solution. But implementing mass vaccination campaigns in large populations within a short period poses many challenges. For example, in Arequipa, Peru, sweeping mass vaccination campaigns conducted yearly over a single weekend have failed to achieve the required ‘herd immunity’ to halt canine rabies transmission. Contrary to the global paradigm of a simultaneous campaign, the 2022 Arequipa rabies campaign was implemented at the sub-district level (patches), with dates of the campaign staggered across 6 months.

Methods
We constructed a stochastic, metapopulation model to examine how the timing of pulsed vaccination campaigns across patches can affect metapopulation dynamics. We explore general metapopulation dynamics for pulsed vaccinations as well as parameterizing the model for canine rabies in Arequipa, Peru. We simulated how the timing of the planned vaccination campaign, staggered over 6 months versus a single yearly pulse, affected the prospects for regional rabies elimination.

Results
Metapopulation dynamics can affect the efficacy of pulsed vaccination campaigns. In the case of Arequipa, Peru, the planned staggered mass dog vaccination campaign has the potential for local elimination with the tradeoffs of increased time to elimination and increased outbreak size due to metapopulation dynamics.

Conclusions
Heterogeneities caused by control strategies enactment at sub-population scales should be accounted for when modeling transmission dynamics. In Arequipa, Peru, although metapopulation dynamics may allow for re-introduction of canine rabies in previously vaccinated patches when mass dog vaccination campaigns are staggered temporally over 6 months, continuous mass vaccination reaching recommended vaccination coverage levels is sufficient to eliminate canine rabies.
Sugar feeding by Aedes albopictus in the Torres Strait, Australia
Authors: Sebayang Boni F. , Russell Tanya L. , Mosby Susannah , Gela Richard , Roeger Darcy L. , van de Straat Bram , Staunton Kyran M. , Burkot Thomas R. 
Published: February 2025

Background
The establishment of Aedes albopictus in the Torres Strait Islands in 2005 is a threat to dengue control in the islands and on mainland Australia. Attractive targeted sugar baits (ATSBs) have been proposed as a control strategy for outdoor mosquitoes like Ae. albopictus. The sugar feeding behaviours of Ae. albopictus was studied to ascertain the potential of ATSBs to mitigate the risk of Ae. albopictus invading mainland Australia from the Torres Strait Islands.

Methodology/Principal Findings
Aedes albopictus was sampled by sweep net collections in village and bushland habitats across two islands both in the mornings and afternoons. Samples were analysed to determine adult abundance as well as fructose prevalence and content by cold-anthrone test. Sampling stations were characterised by vegetation surveys and included the prevalence of flowers and fruit, and canopy cover. Among the 6,186 captured Ae. albopictus, the prevalence of fructose was 31.6% ± 1.4 in males and 30.5% ± 1.2 in females, with fructose averaging 62.9 µg (± 1.4) in fructose-positive Ae. albopictus.

Conclusions
Mosquito sex and collection time were associated with the abundance of Ae. albopictus as well as fructose prevalence and content in Ae. albopictus. Male and female Ae. albopictus exhibited sugar abundance and prevalence comparable to studies where ATSBs were effective suggesting that ATSBs could potentially reduce Ae. albopictus populations in the Torres Strait Islands.