bluuebunny/crossref_metadata_2025_split huggingface dataset:
Potentiation of Exocytosis by Phospholipase C-Coupled G-Protein-Coupled Receptors Requires the Priming Protein Munc13-1
Authors: Bauer Claudia S. , Woolley Robert J. , Teschemacher Anja G. , Seward Elizabeth P. 
Published: January 2007

The vesicle priming protein Munc13-1 is regulated by diacylglycerol (DAG) and is therefore hypothesized to play a role in the control of neurotransmitter release by phospholipase C (PLC)-coupled receptors. We combined voltage-clamp recordings of voltage-gated Ca2+channels (VGCCs) and high-resolution capacitance measurements to investigate the mechanism of receptor-mediated modulation of exocytosis in bovine chromaffin cells. Activation of endogenous H1Gq-protein-coupled receptors (GqPCRs) by histamine potentiated stimulus-coupled secretion despite concurrently inhibiting Ca2+influx through VGCCs. Histamine increased the size of the readily releasable pool of vesicles and in particular a subpool of fusion-competent vesicles localized in close proximity to VGCCs. Pharmacological characterization showed that potentiation of exocytosis depended on the activation of PLC but not protein kinase C. Overexpression of wild-type Munc13-1 by adenoviral infection had no effect on histamine-induced potentiation per se, whereas DAG-insensitive Munc13-1H567Kcompletely abolished it.This is the first endogenous mammalian GqPCR signaling pathway identified that engages Munc13-1 to increase stimulus-coupled secretion by recruiting vesicles to the immediately releasable pool. GqPCRs are therefore able to control exocytosis at the level of SNARE (solubleN-ethylmaleimide-sensitive factor attachment protein receptor) complex formation to produce rapid, short-term potentiation of the secretory output of neurons and endocrine cells.
Energy Absorption Capability of Graded Hybrid Triply Periodic Minimal Surface Structures Based on Fracture Zone Controlling
Authors: Shi Xin , Jiang Weimin , Song Kanghui , Zhang Changdong , Liu Tingting , Liao Wenhe 
Published: March 2024

A novel design strategy of the graded hybrid energy‐absorbing lattice structure is proposed by controlling fracture zone. The primitive (P) and iWP (W) structures are two kinds of triply periodic minimal surface structures with typical deformation and failure modes. Thus, taking the P (stretching‐dominated) and the W structures (bending‐dominated) as object, the deformation and failure mechanisms of two structures are studied. Then, the PWP (i.e., the fracture zone of the P structure filling with the W structure) and WPW (i.e., the fracture zone of the P structure filling with the W structure) structures with uniform and gradient change are designed. The structures are fabricated using stereolithography appearance process and compressed under quasistatic conditions. And the energy absorption capacity comparison of these structures is studied. The experimental results show that the maximum energy absorption efficiency of the PWP structure is 38.85% higher than that of the P structure and the densification energy absorption of the WPW structure is 66.67% higher than that of the W structure. Additionally, gradient design can effectively improve the densification energy absorption of lattice structure. The novel structural design greatly contributes to customized energy absorption design of lattice structures.
Bioprinting Macroporous Hydrogel with Aqueous Two‐Phase Emulsion‐Based Bioink: In Vitro Mineralization and Differentiation Empowered by Phosphorylated Cellulose Nanofibrils
Authors: Wang Qingbo , Karadas Özge , Rosenholm Jessica M. , Xu Chunlin , Näreoja Tuomas , Wang Xiaoju 
Published: March 2024

AbstractAqueous two‐phase emulsion (ATPE)‐based bioinks, a creative innovation for bioprinting, enable the fabrication of complex 3D cell‐laden hydrogels with macroporous structure, which promote cellular activities within the scaffold. However, these bioinks intrinsically lack stability and specific biofunctionality, potentially limiting their application for targeted tissue engineering. This study proposes a new perspective by introducing less than 0.1% phosphorylated cellulose nanofibrils (pCNF), a 1D nanofibril top‐down produced from natural biomasses, into a dextran/methacrylated gelatin (GelMA)‐based ATPE system for extrusion‐based bioprinting of preosteoblastic cells, aiming to fabricate macroporous hydrogels with osteogenic differentiation potential. The pCNF that is selectively partitioned in the GelMA phase can not only improve the emulsion stability and alter the rheological behaviors of the ATPE‐based bioink, but also enhance the damping capacity and mineralization ability of the crosslinked hydrogels. Furthermore, macroporous hydrogels with pCNF demonstrate increased cell activity and higher viability in post‐printing, along with higher alkaline phosphatase activity and osteoblastic gene expression. Importantly, the organized interfaces within the hydrogel facilitate the formation of macroscopic biomineralized nodules in vitro. The incorporation of multifunctional pCNF in the ATPE system significantly boosts the physiochemical and biological performance of the macropore‐forming bioink, transforming them into a suitable platform for engineering in vitro bone models.
Anion Hybridization for Enhanced Broadband Optical Response in Bi‐Doped Photonic Glass and Fiber
Authors: Yang Wangming , Dong Quan , Huang Yupeng , Lin Quanhua , Zhang Ke , Chen Jingfei , Li Xueliang , Zhou Shifeng 
Published: March 2024

AbstractGiven the ever‐increasing demand for transmission capacity in optical fiber communication systems, there is a growing focus on developing active fibers and devices with an optical response across a wider spectrum. Bismuth (Bi)‐doped photonic glasses have attracted significant interest for their exceptional ultra‐broadband optical response, particularly within telecommunication bands. However, developing advanced Bi‐activated materials with robust optical response, high compatibility with commercial transmission systems, and low processing temperature remains a huge challenge. Herein, an anion hybridization strategy is proposed to overcome the aforementioned limitations. The anion hybridization significantly enhances the broadband optical response (≈10 times) of the Bi center following the introduction of fluorine ions, and reduces the transition temperature of the photonic glass (≈200 °C). Moreover, the near‐infrared optical response bandwidth experiences significant broadening, a result attributed to the larger inhomogeneous broadening. Furthermore, successful fabrication of the anion‐hybridized Bi‐activated glass fiber is achieved, demonstrating excellent compatibility with commercial transmission fibers. Additionally, the fabricated fiber exhibits an amplified spontaneous emission and on‐off gain across a broad waveband, further validating its efficacy. These results indicate that anion hybridization offers an effective strategy to optimize the optical and thermal properties of photonic materials to develop novel advanced photonic devices.
High Quality Near‐Infrared Single‐Mode Lasing from γ‐InSe Using a Transferrable Planar Microcavity
Authors: Ge Anping , Sun Liaoxin , Xie Maobin , Cui Henyi , Zhou Dongjie , Ma Lei , Zhang Xin , Huan Yanlin , Tian Haoyang , Jing Wenji , Yao Bimu , Wang Shaowei , Shen Xuechu , Lu Wei 
Published: March 2024

AbstractThe on‐chip near‐infrared (NIR) lasing devices based on van der Waals (vdW) layered materials are highly desired owing to their widespread applications in optoelectronic communication, computing, and sensing. However, the single‐mode NIR lasing devices with superior performance based on vdW layered materials are hard to obtain because of complex and meticulous microcavity structure and the damage to layered materials during preparation. Here, a high‐quality NIR single‐mode lasing device in γ‐phase indium selenide (γ‐InSe) is achieved by using a transferrable planar microcavity. The single‐mode lasing devices based on distributed Bragg reflectors microcavity and super Tamm structure can be simply prepared with quality factors up to 5710 and 3526, respectively. And angle‐resolved spectra show that the lasing device has high directionality with divergence angle <5°. Moreover, the wavelength of lasing device can be tuned ≈30 nm by varying the cavity length via thickness control of γ‐InSe layer. These results not only suggest that γ‐InSe is a promising material for NIR lasing devices, but also present a simple and effective approach for preparing high‐quality lasing devices utilizing other vdW layered materials.
Enhancing Photocurrent Efficiency in Filter‐Less NIR Organic Photodetectors Through a Photomultiplication‐Inducing Perovskite Quantum Dot Interlayer
Authors: Jeong Minyoung , Han Se Gyo , Park Byeongchan , Choi Jinhyeok , Chung Sein , Lee Dongki , Cho Kilwon 
Published: March 2024

AbstractA unique charge‐injection‐narrowing organic photodetector (CIN OPD) is demonstrated for near‐infrared (NIR) range operation. In conventional CIN OPDs, photomultiplication (PM) is achieved within a bulk heterojunction (BHJ) active layer with a donor:acceptor ratio of 100:1. Unfortunately, this approach encounters an issue in which the narrowband detection wavelength is determined solely by an excessive donor quantity. Consequently, the conventional devices face a critical limitation in that their detection range cannot extend to longer wavelengths. However, this limitation has been overcome through a novel mechanism and have fabricated a device that responds at λ = 940 nm. When a perovskite quantum dot interlayer is inserted as the PM‐inducing layer, PM occurs outside the active layer. Therefore, the BHJ active layer is structured with a donor:acceptor ratio of 50:50, resulting in an extension of the detection wavelength to the absorption tail of the narrow‐bandgap acceptor. The device demonstrates a remarkable external quantum efficiency (EQE) of 39 000% with a narrow full‐width at half‐maximum (FWHM) of 98 nm at 60 V. It also has a high specific detectivity and a short response time. The device represents a significant advancement in filter‐free, high‐efficiency photodetectors suitable for optical sensor applications such as light detection and ranging (LiDAR).
Achieving Low Cost and High Performance Flexible CsPbIBr2 Perovskite Photodetectors Arrays with Imaging System via Dual Interfacial Optimization and Structural Design
Authors: Zhao Yue , Jiao Shujie , Yang Song , Wang Dongbo , Gao Shiyong , Wang Jinzhong 
Published: March 2024

AbstractAll‐inorganic metal halide perovskites are competitive candidate as next‐generation flexible photodetectors (PDs) materials due to the remarkable photoelectric properties, enhanced heat stability, and mechanical flexibility. However, constructing low‐cost flexible perovskites PDs with high photodetection performance, good operation stability and mechanical robustness, and manufacturing corresponding flexible PDs array have been a challenge. Herein, the PbCl2 and Zinc(II) Dibenzyldithiocarbamate (Zn(DBDTC)2) are employed to modify bottom and top interface of CsPbIBr2 perovskite layer, respectively, to passivate surface trap state, enhance hydrophobicity and resist phase segregation, and improve interface contact. Consequently, the self‐powered champion PDs with low‐cost Bi2Te3 electrode reach a responsivity of 0.38 A W−1 and a maximum detectivity of 3.44 × 1013 Jones at 570 nm, and maintain the outstanding long‐term and operational stability without additional encapsulation. Meanwhile, the HTL‐free flexible PDs based on low‐temperature CsPbIBr2 perovskite layer exhibit impressive photodetection performance and mechanical robustness in bending test. More importantly, the flexible PDs array (5 × 5 pixels) and corresponding imaging system are fabricated with uniform and clear imaging results under flatting and bending state. This research is of great significance for the application of stable and high‐performance inorganic perovskites PDs and flexible array imaging systems.
Self‐Assembled 2D Sheets of an Amphiphilic Sexiphenyl Exhibit Intense Polarized Blue Emission in the Solid State
Authors: Mondal Pramita , Hill Jonathan P. , Manna Gouranga , De Dalui Sharmistha , Neal Edward A. , Richards Gary. J. , Ariga Katsuhiko , Yamauchi Yusuke , Shrestha Lok Kumar , Acharya Somobrata 
Published: March 2024

AbstractDistinct from inorganic 2D nanomaterials (e.g., graphene, MoS2, etc,), self‐assembled organic 2D materials are significant due to the unique advantages of fine structure tunability and functionality. Electronically and photofunctionally‐active compounds (e.g., pentacene, p‐sexiphenyl) are highly attractive for applications but are limited by difficult handling and the existence of only a few native structures of the compounds. Here, it is shown that a p‐sexiphenyl derivative incorporated into 2D multilamellar sheets with chromophores oriented in the layers exhibits a significant polarization of photoluminescence. Highly emissive, amphiphilic sexiphenyl with hydrophilic/hydrophobic groups self‐assembles at air–water interface with chromophore orientation in the resulting films being controlled by monolayer compression. Large‐area multilamellar structures with p‐sexiphenyl chromophores highly oriented against the 2D plane are prepared by consecutive film transfers. The 2D sheets exhibit significant polarization of photoluminescence with polarization ratio 0.8 between orthogonal in‐plane axes. Notably, multilamellar structures cannot be established for the same p‐sexiphenyl using thermotropic processing thus emphasizing the importance of the 2D sheet multilayer synthesis process. Amphiphile design can be applied for the tailored synthesis of large‐area multilayered 2D sheets and used to construct highly efficient light‐emitting devices.
Neurotoxic Microglial Activation via IFNγ‐Induced Nrf2 Reduction Exacerbating Alzheimer's Disease
Authors: Kang You Jung , Hyeon Seung Jae , McQuade Amanda , Lim Jiwoon , Baek Seung Hyun , Diep Yen N. , Do Khanh V. , Jeon Yeji , Jo Dong‐Gyu , Lee C. Justin , Blurton‐Jones Mathew , Ryu Hoon , Cho Hansang 
Published: March 2024

AbstractMicroglial neuroinflammation appears to be neuroprotective in the early pathological stage, yet neurotoxic, which often precedes neurodegeneration in Alzheimer's disease (AD). However, it remains unclear how the microglial activities transit to the neurotoxic state during AD progression, due to complex neuron‐glia interactions. Here, the mechanism of detrimental microgliosis in AD by employing 3D human AD mini‐brains, brain tissues of AD patients, and 5XFAD mice is explored. In the human and animal AD models, amyloid‐beta (Aβ)‐overexpressing neurons and reactive astrocytes produce interferon‐gamma (IFNγ) and excessive oxidative stress. IFNγ results in the downregulation of mitogen‐activated protein kinase (MAPK) and the upregulation of Kelch‐like ECH‐associated Protein 1 (Keap1) in microglia, which inactivate nuclear factor erythroid‐2‐related factor 2 (Nrf2) and sensitize microglia to the oxidative stress and induces a proinflammatory microglia via nuclear factor kappa B (NFκB)‐axis. The proinflammatory microglia in turn produce neurotoxic nitric oxide and proinflammatory mediators exacerbating synaptic impairment, phosphorylated‐tau accumulation, and discernable neuronal loss. Interestingly, recovering Nrf2 in the microglia prevents the activation of proinflammatory microglia and significantly blocks the tauopathy in AD minibrains. Taken together, it is envisioned that IFNγ‐driven Nrf2 downregulation in microglia as a key target to ameliorate AD pathology.
Integration Analysis of Single‐Cell Multi‐Omics Reveals Prostate Cancer Heterogeneity
Authors: Bian Xiaojie , Wang Wenfeng , Abudurexiti Mierxiati , Zhang Xingming , Ma Weiwei , Shi Guohai , Du Leilei , Xu Midie , Wang Xin , Tan Cong , Sun Hui , He Xiadi , Zhang Chenyue , Zhu Yao , Zhang Min , Ye Dingwei , Wang Jianhua 
Published: March 2024

AbstractProstate cancer (PCa) is an extensive heterogeneous disease with a complex cellular ecosystem in the tumor microenvironment (TME). However, the manner in which heterogeneity is shaped by tumors and stromal cells, or vice versa, remains poorly understood. In this study, single‐cell RNA sequencing, spatial transcriptomics, and bulk ATAC‐sequence are integrated from a series of patients with PCa and healthy controls. A stemness subset of club cells marked with SOX9highARlow expression is identified, which is markedly enriched after neoadjuvant androgen‐deprivation therapy (ADT). Furthermore, a subset of CD8+CXCR6+ T cells that function as effector T cells is markedly reduced in patients with malignant PCa. For spatial transcriptome analysis, machine learning and computational intelligence are comprehensively utilized to identify the cellular diversity of prostate cancer cells and cell‐cell communication in situ. Macrophage and neutrophil state transitions along the trajectory of cancer progression are also examined. Finally, the immunosuppressive microenvironment in advanced PCa is found to be associated with the infiltration of regulatory T cells (Tregs), potentially induced by an FAP+ fibroblast subset. In summary, the cellular heterogeneity is delineated in the stage‐specific PCa microenvironment at single‐cell resolution, uncovering their reciprocal crosstalk with disease progression, which can be helpful in promoting PCa diagnosis and therapy.
Deficiency of Nuclear Receptor Coactivator 3 Aggravates Diabetic Kidney Disease by Impairing Podocyte Autophagy
Authors: Xie Yaru , Yuan Qian , Cao Xinyi , Qiu Yang , Zeng Jieyu , Cao Yiling , Xie Yajuan , Meng Xianfang , Huang Kun , Yi Fan , Zhang Chun 
Published: March 2024

AbstractNuclear receptors (NRs) are important transcriptional factors that mediate autophagy, preventing podocyte injury and the progression of diabetic kidney disease (DKD). However, the role of nuclear receptor coactivators that are powerful enhancers for the transcriptional activity of NRs in DKD remains unclear. In this study, a significant decrease in Nuclear Receptor Coactivator 3 (NCOA3) is observed in injured podocytes caused by high glucose treatment. Additionally, NCOA3 overexpression counteracts podocyte damage by improving autophagy. Further, Src family member, Fyn is identified to be the target of NCOA3 that mediates the podocyte autophagy process. Mechanistically, NCOA3 regulates the transcription of Fyn in a nuclear receptor, PPAR‐γ dependent way. Podocyte‐specific NCOA3 knockout aggravates albuminuria, glomerular sclerosis, podocyte injury, and autophagy in DKD mice. However, the Fyn inhibitor, AZD0530, rescues podocyte injury of NCOA3 knockout DKD mice. Renal NCOA3 overexpression with lentivirus can ameliorate podocyte damage and improve podocyte autophagy in DKD mice. Taken together, the findings highlight a novel target, NCOA3, that protects podocytes from high glucose injury by maintaining autophagy.
A Multi‐foci Sparse‐Aperture Metalens
Authors: Xu Borui , Wei Wei , Tang Ping , Shao Jingzhu , Zhao Xiangyu , Chen Bo , Dong Shengxiang , Wu Chongzhao 
Published: March 2024

AbstractMulti‐foci lenses are essential components for optical communications, virtual reality display and microscopy, yet the bulkiness of conventional counterparts has significantly hindered their widespread applications. Benefiting from the unprecedented capability of metasurfaces in light modulation, metalenses are able to provide multi‐foci functionality with a more compact footprint. However, achieving imaging quality comparable to that of corresponding single‐foci metalenses at each focal point poses a challenge for existing multi‐foci metalenses. Here, a polarization‐independent all‐dielectric multi‐foci metalens is proposed and experimentally demonstrated by spatially integrating single‐foci optical sparse‐aperture sub‐metalenses. Such design enables the metalens to generate multiple focal points, while maintaining the ability to capture target information comparable to that of a single‐foci metalens. The proposed multi‐foci metalens is composed of square‐nanohole units array fabricated by two‐photon polymerization. The focusing characteristic and imaging capability are demonstrated upon the illumination of an unpolarized light beam. This work finds a novel route toward multi‐foci metalenses and may open a new avenue for dealing with the trade‐off between multi‐foci functionality and high‐quality imaging performance.
Neutrophil to lymphocyte ratio in captive olive baboons (Papio anubis): The effects of age, sex, rearing, stress, and pregnancy
Authors: Neal Sarah J. , Achorn Angela M. , Schapiro Steven J. , Hopkins William D. , Simmons Joe H. 
Published: March 2024

AbstractIn apes and humans, neutrophil to lymphocyte ratio (NLR) can be used as a predictive indicator of a variety of clinical conditions, longevity, and physiological stress. In chimpanzees specifically, NLR systematically varies with age, rearing, sex, and premature death, indicating that NLR may be a useful diagnostic tool in assessing primate health. To date, just one very recent study has investigated NLR in old world monkeys and found lower NLR in males and nursery‐reared individuals, as well as a negative relationship between NLR and disease outcomes. Given that baboons are increasingly used as research models, we aimed to characterize NLR in baboons by providing descriptive data and examinations of baboon NLR heritability, and of the relationships between NLR, age, rearing, and sex in 387 olive baboons (Papio anubis) between 6 months and 19 years of age. We found that (1) mother‐reared baboons had higher NLRs than nursery‐reared baboons; (2) females had higher NLRs than males; and (3) there was a quadratic relationship between NLR and age, such that middle‐aged individuals had the highest NLR values. We also examined NLR as a function of transport to a new facility using a subset of the data. Baboons exhibited significantly higher transport NLRs compared to routine exam NLRs. More specifically, adult baboons had higher transport NLRs than routine NLRs, whereas juveniles showed no such difference, suggesting that younger animals may experience transport stress differently than older animals. We also found that transport NLR was heritable, whereas routine NLR was not, possibly suggesting that stress responses (as indicated in NLR) have a strong genetic component. Consistent with research in humans and chimpanzees, these findings suggest that NLR varies with important biological and life history variables and that NLR may be a useful health biomarker in baboons.
Catalytic Ring Expanding Difluorination: An Enantioselective Platform to Access β,β‐Difluorinated Carbocycles
Authors: Ruyet Louise , Roblick Christoph , Häfliger Joel , Wang Zi‐Xuan , Stoffels Tobias Jürgen , Daniliuc Constantin G. , Gilmour Ryan 
Published: March 2024

AbstractCyclic β,β‐difluoro‐carbonyl compounds have a venerable history as drug discovery leads, but limitations in the synthesis arsenal continue to impede chemical space exploration. This challenge is particularly acute in the arena of fluorinated medium rings where installing the difluoromethylene unit subtly alters the ring conformation by expanding the internal angle (∠C−CF2−C>∠C−CH2−C): this provides a handle to modulate physicochemistry (e.g. pKa). To reconcile this disparity, a highly modular ring expansion has been devised that leverages simple α,β‐unsaturated esters and amides, and processes them to one‐carbon homologated rings with concomitant geminal difluorination (6 to 10 membered rings, up to 95 % yield). This process is a rare example of the formal difluorination of an internal alkene and is enabled by sequential I(III)‐enabled O‐activation. Validation of enantioselective catalysis in the generation of unprecedented medium ring scaffolds is reported (up to 93 : 7 e.r.) together with X‐ray structural analyses and product derivatization.