bluuebunny/crossref_metadata_2025_split huggingface dataset:
RBM15 is a novel prognostic biomarker and correlates with immune cell infiltration in lung adenocarcinoma
Authors: Ma Mingsheng , Wang Wei , Li Li , Wang Xiaoyan , Huang Qiubo , Zhou Chen , Ye Lianhua 
Published: April 2024

Abstract
Background and Aims: This study aimed to evaluate RBM15 gene expression and the potential effects as a biomarker in LUAD progression.
Materials and Methods: The RNA sequencing (RNA-seq) data and clinical data of patients with LUAD were acquired from The Cancer Genome Atlas (TCGA) databases. Kaplan-Meier (K-M) curves were generated to investigate the relationship between RBM15 and the prognosis of patients with LUAD. Gene Ontology (GO) and Reactome enrichment analyses were performed using the “cluster Profiler” R package. Finally, the Tumor Immune Estimation Resource (TIMER)database and CIBERSORT algorithm were used to assess the correlations between RBM15 expression and immune infiltration in LUAD.
Results: RBM15 was upregulated in tumor tissue, and it was regarded as an independent prognostic factor in LUAD. The genes co-expressed with RBM15 were closely related to cell cycle checkpoints and M phase signaling pathways. Furthermore, there was a significant correlation between RBM15 gene expression and immune infiltration in LUAD.
Conclusion: Our data suggested that RBM15 is critical in LUAD progression, is associated with tumor immune infiltration and served as a valuable potential diagnostic biomarker in patients.
Evaluating the implementation of the blood pressure monitoring program based on smart wearable devices (BPMP-SWD) of intravenous thrombolysis for acute ischemic stroke: Protocol for a randomized controlled trial
Authors: Li Suai , Zhao Jie , Liu Mengrao , Ma Gaoting , Han Jinming , Tian Siying , Wang Yuxin , Yang Ting , Chang Hong 
Published: April 2024

Abstract
Background
It is recommended that blood pressure (BP) in patients with acute ischemic stroke (AIS) should be monitored closely before and until 24 hours after thrombolysis. The guideline adherence to BP monitoring in clinical practice can be influenced by a variety of factors. Interventions integrated with knowledge on influencing factors have been widely used to improve guideline adherence.
Aim
To evaluate if the BP monitoring program based on smart wearable devices (BPMP-SWD) can improve the adherence of intravenous thrombolytic (IVT) care in patients with AIS.
Methods
This study is a prospective, open-label, endpoint-blinded, single-center, randomized controlled trial, randomized in a 1:1 ratio to either the control or intervention group at a comprehensive stroke center. The BP in the control group will be monitored using a conventional bedside monitoring device and the intervention group will be monitored using a smart wearable device. The primary outcome is the guideline adherence to BP monitoring for IVT. The secondary outcomes include the feasibility, fidelity and acceptance of smart wearable devices. Based on pilot data, a total of 160 patients with AIS undergoing IVT will be recruited.
Discussion
This study may provide novel evidence on improving the adherence to intravenous thrombolytic BP monitoring and support the wider implementation of smart wearable devices-based intervention to promote evidence-based practice.
Trial registration
The Chinese Clinical Trial Registry ChiCTR2300069063 (Date of registration 03.06.2023)
Mapping the threads: Geospatial Exploration of Shared Modifiable Tuberculosis Disease Risks in a clinic-based population
Authors: Asare-Baah Michael , Luong Tan M. , Afriyie-Mensah Jane , Kwarteng Ernest , Domotey Charles , Arthur Nellie , Zoungrana Magalie , Mireku-Apah Sheila , Ganu Honesty , Omari Michael Amo , Sackey Adelaide , Kwara Awewura , Séraphin Marie Nancy 
Published: April 2024

Abstract
Background In emerging economies experiencing rapid sociodemographic transitions and historically high tuberculosis (TB) prevalence, effective TB control requires acknowledging the evolving socio-behavioral characteristics of diverse patient populations shaping community-level TB risk. This study aimed to explore the spatial distribution and clustering of shared modifiable clinical and social risk factors for TB in a clinic-based population in Accra, Ghana.Methods We prospectively enrolled new and previously treated TB patients between June 2022 and July 2023. At diagnosis, patients provided informed consent to collect their residential coordinates and completed a questionnaire assessing their demographic and modifiable clinical and social risks for TB. We used geospatial scan statistics to describe the spatial distribution of cases and PERMANOVA to examine the correlation between spatial proximity and shared socio-behavioral risks, with a 1.5 square kilometer threshold defining significant residential proximity.Results The study population (N = 150) was predominantly male (68.0%) and of working age (80.0% aged 25–64 years), with half the sample engaged in unskilled labor (51.3%). Approximately one-third reported heavy alcohol (36.0%) and recreational drug use (26.7%) in the past year. Fifteen percent were HIV-positive, of whom more than 80% were diagnosed at the time of TB diagnosis. Local Moran's I statistics revealed spatial clusters of TB cases in separate sections of the study area. Unskilled labor, recreational drug use, and a history of cough in patients’ social contacts were significantly associated with residential proximity, explaining 1.26% of the variance in our model (F = 1.89, R^2 = 1.3%, p = 0.004).Conclusions Shared modifiable risks, including unskilled labor, recreational drug use, and close contact with TB, exhibited spatial clustering, suggesting their potential to enhance TB disease progression and transmission in this setting. Targeted interventions addressing these socio-behavioral risks within identified hotspots may improve TB control efforts.
Supporting shared-decision making for anticoagulation initiation in people with advanced kidney disease
Authors: Parker Kathrine , Needham Abigail , Thachil Jecko , Mitra Sandip , Lewis Penny 
Published: April 2024

Abstract

Background
Patients with chronic kidney disease are at increased risk of thrombotic and bleeding episodes making anticoagulant treatment decisions challenging. Currently, there are no decision support tools for patients with CKD regarding anticoagulant therapy. This work aimed to co-produce patient materials to support shared-decision making of anticoagulant use in advanced chronic kidney disease.
Methods
Focus groups were used to explore the views of kidney patients towards anticoagulant prescribing. Data was thematically analysed based on Makoul and Clayman’s model of shared-decision making. Co-production was used to develop patient material in conjunction with kidney patients over three meetings.
Results
A list of questions, to be used by patients when initiated on an anticoagulant, were co-produced. These questions were based upon participants’ experiences of the various stages of shared-decision making within the context of anticoagulant use in advanced chronic kideny disease, EGFR < 30ml/min/1.73m2. Of particular importance to participants was the provision of information relating to treatment risks and follow up arrangements.
Conclusion
Shared-decision making is important when initiating medication to ensure the best outcomes for patients, yet it can be difficult to engage in shared-decision making without prompts or guidance This co-produced patient guide could be used to support shared-decision making for anticoagulant initiation in patients with advanced chronic kidney disease.
Endothelial and neuronal engagement by AAV-BR1 alleviates cholesterol deposition in a mouse model of Niemann-Pick type C2
Authors: Rasmussen Charlotte Laurfelt Munch , Heegaard Christian Würtz , Thomsen Maj Schneider , Hede Eva , Laczek Bartosz , Körbelin Jakob , Thomsen Louiza Bohn , Schwaninger Markus , Moos Torben , Burkhart Annette 
Published: April 2024

Abstract

Background Patients with the genetic disorder Niemann-Pick type C2 disease (NP-C2) suffer from lysosomal accumulation of cholesterol causing both systemic and severe neurological symptoms. In a murine NP-C2 model, otherwise successful intravenous Niemann-Pick C2 protein (NPC2) replacement therapy fails to alleviate progressive neurodegeneration as infused NPC2 is unable to cross the blood-brain barrier (BBB). Genetic modification of brain endothelial cells (BECs) is thought to enable secretion of recombinant proteins thereby overcoming the restrictions of the BBB. We hypothesized that BBB-directed gene therapy using the AAV-BR1-NPC2 vector would transduce both BECs and neurons in a mouse model of NP-C2 (Npc2-/-).Methods Six weeks old Npc2-/- mice were intravenously injected with the AAV-BR1-NPC2 vector. Post-mortem analyses included gene expression analyses, determination of NPC2 transduction in the CNS, and co-detection of cholesterol with NPC2 in neurons.Results The vector exerted tropism for BECs and neurons resulting in a widespread NPC2 distribution in the brain with a concomitant reduction of cholesterol in adjacent neurons, presumably not transduced by the vector.Conclusion The data suggests cross-correcting gene therapy to the brain via delivery of NPC2 from BECs and neurons.
Factor associated with women’s choice for place of delivery in a selected municipality of Madesh Province, Nepal
Authors: Yadav Neelima , Paneru Damaru Prasad , Shrestha Niranjan , Yadav Rajiv Ranjan 
Published: April 2024

Abstract

Background
Maternal mortality from pregnancy and childbirth remains a major public health problem in Nepal. Government of Nepal has been continuously making effort to reduce maternal mortality through its different programs, policies, and incentives schemes. Evidence suggests that the reasons for slow progress in MNH outcomes are partly contributed by equity gaps in access to services, and utilization of poor-quality health services. Thus, this study aims to assess the preference and the reasons for choices of delivery place among the women in Rautahat.
Methods
A quantitative, cross-sectional study was carried out among 278 married women of reproductive age group in Paroha Municipality who had given at least one birth within past 12 months. Face to face interview was conducted using structured questionnaire after obtaining ethical approval from IRC Pokhara university, health section of Paroha Municipality and written consent from participants. Epi-data for data entry and SPSS was used for further analysis. Univariate, bivariate and multivariate analysis were carried out in order to obtain results.
Results
The study found that 89.6% women preferred health institution as ideal place of delivery. And 84.9% had institutional delivery. Similarly, factor such as women occupation (AOR = 5.901, 95% CI: 1.099–31.683) ANC visit (AOR = 8.491, 95% CI: 1.975–36.508), women preferred delivery place (AOR = 4.570, 95% CI: 0.614–5.972), husband preferred delivery place (AOR = 43.947, 95% CI: 8.573-225.279), delivery at preferred place (AOR = 8.927, 95% CI: 2.233–35.690) and use of nearest health facility (AOR = 5.473, 95% CI: 1.221–24.525) were major factor influencing place of delivery.
Conclusion
To ensure equitable and accessible services and improved utilization of institutional delivery it is important to identify what progress has been achieved, whether there are vulnerable and disadvantaged groups that need specific attention and the key factors affecting the utilization of institutional delivery services. Also, to promote institutional delivery and improve maternal and child health outcomes, healthcare systems should consider preferences and factors that could affect to ensure that quality of maternity care.
Control of CDH1 and IDH1mut glioblastoma cell cycle by D-2- hydroxyglutarate
Authors: You Meng-Qi , Xu Wei 
Published: April 2024

Abstract
Glioblastoma (GBM) is a prevalent and lethal primary brain tumor. Patients with the IDH1R132H (isocitrate dehydrogenase 1) mutation exhibit extended survival and aneuploidy, yet the underlying mechanisms are unclear. Here, we reveal that the accumulation of D-2-hydroxyglutarate (2-HG) produced by IDH1R132H mutation induces the degradation of Fizzy-related protein1(FZR1 or CDH1) by inhibiting prolyl hydroxylase EGLN2 activity. CDH1 levels are stabilized by α-KG and oxygen, independent of HIF-1α, through EGLN2-mediated hydroxylation. This novel mechanism represents the first instance of prolyl hydroxylation stabilizing a protein. The 2-HG-EGLN2-CDH1 axis induces mitotic arrest and cell growth inhibition, potentially contributing to the extended survival observed in patients with IDH1R132H mutant GBM.
Association between vitamin D levels and physical activity: A cohort study
Authors: Javid Mohammadreza , Joukar Farahnaz , Emamhadi Amirreza , Faraji Niloofar , Zeinali Tahereh , Maroufizadeh Saman , Naghipour Mohammadreza , Mansour-Ghanaei Fariborz 
Published: April 2024

Abstract

Introduction:
A surprisingly high prevalence of vitamin D (vit D) deficiency has recently been reported worldwide. This study investigated the association between vit D levels and physical activity in the Prospective Epidemiological Research Studies in (PERSIAN) cohort study.
Methods
This cross-sectional study has been conducted on 9522 participants aged 35 to 70. The serum vit D concentrations were measured, and participants were divided into three tertiles of low, moderate, and high levels according to the metabolic equivalent of task (MET). The cutoff level for vit D deficiency was considered ≤ 12 ng/ml. All data were analyzed using SPSS version 26 with a significant level of less than 0.05.
Results
About 4428 participants were male, and the mean age of participants was 51.46 ± 8.91 years. The prevalence of vit D deficiency was 22.8% and more common in women than men (23.8% vs. 21.7%). The prevalence of vit D deficiency decreased with increasing physical activity (P < 0.001). Vit D deficiency is significantly associated with increasing body mass index (BMI) (P < 0.05). In the adjusted model for vit D supplements and sociodemographic characteristics, the participants with low physical activity had a higher risk of vit D deficiency than participants with high physical activity.
Conclusions
According to our findings, vit D deficiency was associated with a reduced frequency of participation in all forms of physical activity.
A survey on the attitude and willingness of Chinese cancer patients toward a COVID-19 booster vaccine
Authors: Yao Man , Wang Peng , Wang Meng , Zheng Sheng-qi , Xu Xiao-wan , Zhang Qing-mei , Yu Qin , Qin Hao-ran , Meng Yong-bin , Hong Jing 
Published: April 2024

Abstract
Objective
The 2019 novel coronavirus is still rapidly mutating and spreading worldwide, posing a great global health challenge, especially vulnerable groups, including cancer patients. Studies comparing the clinical outcomes of cancer patients receiving or not receiving the 2019 coronavirus disease (COVID-19) vaccines have not yet shown significant benefits. Therefore, this study mainly focused on the attitudes and willingness of cancer patients toward COVID-19 booster vaccines and to distinguish independent predictors that may impact the vaccination of cancer patients.
Methods
An online cross-sectional study was conducted between July 1 2022 and January 1 2023 in China. Patients were selected using a convenience sampling method, and a self-reporting questionnaire was developed based on a literature review to evaluate the attitude of cancer patients towards the intensive use of COVID-19 vaccines and the willingness of patients to accept the COVID-19 vaccine. Independent predictors associated with accepting a COVID-19 booster vaccine were determined through multivariate logistic regression.
Results
A total of 1055 participants were included in this study. The vaccine acceptance rate was 56.11% (592/1055), while the hesitancy rate was 43.89% (463/1055). Frequent smoking, those who had received two doses of the COVID-19 vaccine, belief that the COVID-19 booster vaccine was necessary, belief that the COVID-19 booster vaccine was safe and effective, willingness to pay for the COVID-19 booster vaccine, and recommendation of COVID-19 booster vaccine by friends and family were contributing factors for vaccine acceptance.
Conclusion
This study recommends developing healthcare policies appropriate for cancer patients to facilitate their acceptance of vaccines, such as publishing the safety and effectiveness of vaccines, providing individualized treatment options after adequate risk assessment and developing specific COVID-19 vaccines suitable for cancer patients.
Death anxiety and its relationship with the perception of aging in older adults visiting comprehensive healthcare centers: Evidence from a cross-sectional study in southern Iran
Authors: Ahmadidarrehsima Sudabeh , Rafati Foozieh , Shafiei Shohreh , Mehni Esmeil Barkhori , Abbasi Mohsen , Mashayekhi Fatemeh , Salari Nasibeh 
Published: April 2024

Abstract

Background and Aim:
Death anxiety is one of the most important developments in old age, which reduces the general functioning and psychological well-being of older adults and changes people’s perception of aging. The present study aimed to examine death anxiety and its relationship with the perception of aging in older adults visiting comprehensive healthcare centers in southern Iran.
Materials and Methods
This study was conducted using a correlational cross-sectional design. The research population included all elderly people over 60 years old supported by a university of medical sciences and medical services in southern Iran in 2021. The participants in the research sample were 231 older adults selected using cluster sampling. The data were collected using the Templer Death Anxiety Scale (DAS) and Aging Perception Questionnaire (APQ). The collected data were analyzed using descriptive statistics and t-test, ANOVA, Spearman’s correlation test, Mann-Whitney U test, and Kruskal-Wallis test with SPSS software (version 23). A significance level of less than 0.05 was considered in this study.
Results
The participants’ mean age was 72.56 ± 9.04 years. Besides, most of the participants (129 persons (55.8%) were female, 177 persons (76.6%) were married, 118 persons (51.1%) were illiterate, and more than half of them had a monthly income level of less than one million Tomans. The mean score of death anxiety and perception of aging was 6.39 ± 4.06 and 110.29 ± 18.40, respectively. Moreover, there was a positive and significant relationship between death anxiety and perception of aging (r = 0.23; P < 0.05) in older adults. The data also indicated that death anxiety and perception of aging had a significant relationship with the place of residence, age, and the number of children (P < 0.05).
Conclusion
The present study showed that the older adults in this study experienced a moderate level of death anxiety. Furthermore, since death anxiety had a direct and significant relationship with the perception of aging, innovative interventions can be conducted to educate older adults to cope directly with their death anxiety and improve their mental health. In addition, the related authorities need to devise effective plans and policies to reduce death anxiety and improve positive perceptions of older adults about aging.
Staphylococcus aureus-expressed acetolactate synthase enhances the biosynthesis of branched-chain amino acids and is linked to insulin resistance in type 2 diabetes in South China
Authors: Liang Tingting , Jiang Tong , Liang Zhuang , Li Longyan , Wu Lei , Gao He , Zhao Hui , Zhang Ni , Dong Bo , Xie Xinqiang , Wu Qingping , Gu Bing 
Published: April 2024

Abstract
Background An increase in branched-chain amino acid (BCAA) levels can result in insulin resistance at different stages of type 2 diabetes (T2D), however, the causes of this increase are unclear.
Methods We performed metagenomics and metabolomics profiling in patients with prediabetes (PDM), newly diagnosed diabetes (NDDM), and post-medication type 2 diabetes (P2DM) to investigate whether altered gut microbes and metabolites could explain the specific clinical characteristics of different disease stages of T2D.
Results Here we identify acetolactate synthase (ALS) a BCAA biosynthesis enzyme in Staphylococcus aureus as a cause of T2D insulin resistance. Compared with healthy peoples, patients with PDM, NDDM, and P2DM groups, especially in P2DM group, have increased faecal numbers of S. aureus. We also demonstrated that insulin administration may be a risk factor for S. aureus infection in T2D. The presence of ALS-positive S. aureuscorrelated with the levels of BCAAs and was associated with an increased fasting blood glucose (FBG) and insulin resistance. Humanized microbiota transplantation experiment indicated that ALS contributes to disordered insulin resistance mediated by S. aureus. We also found that S. aureus phage can reduced the FBG levels and insulin resistance in db/db mice.
Conclusions Above all results suggest that the BCAAs biosynthesis increasing bacteria and ALS enzymes are potential intervention targets for the glucose homeostasis in T2D insulin resistance, opening a new therapeutic avenue for the prevention or treatment of diabetes.
miR-1, miR-133a, miR-29b and Skeletal Muscle Fibrosis in Chronic Limb-Threatening Ischaemia.
Authors: Keane Alan , Nogues Clara Sanz , Jayasooriya Dulan , Creane Michael , Chen Xizhe , Lyons Caomhán , Sikri Isha , Goljanek-Whysal Katarzyna , O'Brien Timothy 
Published: April 2024

Abstract

Chronic limb-threatening ischaemia (CLTI), the most severe manifestation of peripheral arterial disease (PAD), is associated with a poor prognosis and high amputation rates. Despite novel therapeutics approaches being investigated, no significant clinical benefits habe been observed yet. Understanding the molecular pathways of skeletal muscle dysfunction in CLTI is crucial for designing successful treatments. This study aimed to identify miRNAs dysregulated in muscle biopsies from PAD cohorts. Using MIcroRNA ENrichment TURned NETwork (MIENTURNET) on a publicly accessible RNA-sequencing database of PAD cohorts, we identified a list of miRNAs that were over-represented among the upregulated differentially expressed genes (DEGs) in CLTI. Next, we validated the altered expression of these miRNAs and their targets in mice with hindlimb ischaemia (HLI). Our results showed a significant downregulation in miR-1, miR-133a, and miR-29b leves in the ischaemic limbs versus the contralateral non-ischaemic limbs. A miRNA target protein-protein interaction network identified extracellular matrix components, including collagen-1a1, -3a1, and − 4a1, fibronectin-1, fibrin-1, matrix metalloproteinase-2 and − 14, and Sparc, which were upregulated in the ischaemic muscle of mice. This is the first study to identify miR-1, miR-133a, and miR-29b as potential contributors to fibrosis and vascular pathology in CLTI muscle, which supports their potential as novel therapeutic agents.