bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pediatric-type high-grade gliomas with PDGFRA amplification in adult patients with Li-Fraumeni syndrome: clinical and molecular characterization of three cases
Authors: Kibe Yuji , Ohka Fumiharu , Aoki Kosuke , Yamaguchi Junya , Motomura Kazuya , Ito Eiji , Takeuchi Kazuhito , Nagata Yuichi , Ito Satoshi , Mizutani Nobuhiko , Shiba Yoshiki , Maeda Sachi , Nishikawa Tomohide , Shimizu Hiroki , Saito Ryuta 
Published: April 2024

AbstractLi-Fraumeni syndrome (LFS) is an autosomal dominant tumor predisposition syndrome caused by heterozygous germline mutations or deletions in the TP53 tumor suppressor gene. Central nervous system tumors, such as choroid plexus tumors, medulloblastomas, and diffuse gliomas, are frequently found in patients with LFS. Although molecular profiles of diffuse gliomas that develop in pediatric patients with LFS have been elucidated, those in adults are limited. Recently, diffuse gliomas have been divided into pediatric- and adult-type gliomas, based on their distinct molecular profiles. In the present study, we investigated the molecular profiles of high-grade gliomas in three adults with LFS. These tumors revealed characteristic histopathological findings of high-grade glioma or glioblastoma and harbored wild-type IDH1/2 according to whole exome sequencing (WES). However, these tumors did not exhibit the key molecular alterations of glioblastoma, IDH-wildtype such as TERT promoter mutation, EGFR amplification, or chromosome 7 gain and 10 loss. Although WES revealed no other characteristic gene mutations or copy number alterations in high-grade gliomas, such as those in histone H3 genes, PDGFRA amplification was found in all three cases together with uniparental disomy of chromosome 17p, where the TP53 gene is located. DNA methylation analyses revealed that all tumors exhibited DNA methylation profiles similar to those of pediatric-type high-grade glioma H3-wildtype and IDH-wildtype (pHGG H3-/IDH-wt), RTK1 subtype. These data suggest that high-grade gliomas developed in adult patients with LFS may be involved in pHGG H3-/IDH-wt. PDGFRA and homozygous alterations in TP53 may play pivotal roles in the development of this type of glioma in adult patients with LFS.
Beyond inundation: a comprehensive assessment of sea level rise impact on coastal cultural heritage in China
Authors: Chen Zihua , Gao Qian , Li Xiaowei , Yang Xiaohui , Wang Zhenbo 
Published: April 2024

Abstract
The rise in sea levels, driven by global climate change, poses a significant threat to cultural heritage in coastal regions. Traditional risk assessment methods, focusing on direct inundation, often fail to consider the crucial impact of socio-economic factors, which are significantly vulnerable to sea level rise. To bridge this gap, this study introduces an innovative Sea Level Rise Cultural Heritage Impact Assessment Model (SLR-CHIA Model), a novel approach that integrates both land inundation and socio-economic aspects. This comprehensive model evaluates potential risks to various types of cultural heritage in coastal China, including intangible cultural heritage, relics, and traditional villages. The study’s findings are striking: (1) About 7.79% of coastal villages, 53.94% of relics, and 2.53% of intangible cultural heritage are potentially at high risk in a 100-year sea level rise event; (2) Relics in the Eastern coast and villages in the Southern coast are most vulnerable; (3) Different types of cultural heritage rely on diverse principal factors for protection, resulting in varied risk levels under sea level rise conditions. The SLR-CHIA Model provides a vital methodological framework for evaluating cultural heritage risks in other global regions.
State cannabis laws and cannabis positivity among fatally injured drivers
Authors: Leavitt Thea Clare , Chihuri Stanford , Li Guohua 
Published: April 2024

Abstract
Background
As of November 8, 2023, 24 states and the District of Columbia have legalized cannabis for both recreational and medical use (RMCL-states), 14 states have legalized cannabis for medical use only (MCL-states) and 12 states have no comprehensive cannabis legislation (NoCL-states). As more states legalize cannabis for recreational use, it is critical to understand the impact of such policies on driving safety.

Methods
Using the 2019 and 2020 Fatality Analysis Reporting System data, we performed multivariable logistic regression modeling to explore the association between state level legalization status and cannabis positivity using toxicological testing data for 14,079 fatally injured drivers. We performed a sensitivity analysis by including multiply imputed toxicological testing data for the 14,876 eligible drivers with missing toxicological testing data.

Results
Overall, 4702 (33.4%) of the 14,079 fatally injured drivers tested positive for cannabis use. The prevalence of cannabis positivity was 30.7% in NoCL-states, 32.8% in MCL-states, and 38.2% in RMCL-states (p < 0.001). Compared to drivers fatally injured in NoCL-states, the adjusted odds ratios of testing positive for cannabis were 1.09 (95% confidence interval: 0.99, 1.19) for those fatally injured in MCL-states and 1.54 (95% confidence interval: 1.34, 1.77) for those fatally injured in RMCL-states. Sensitivity analysis yielded similar results.

Conclusions
Over one-third of fatally injured drivers tested positive for cannabis use. Drivers fatally injured in states with laws permitting recreational use of cannabis were significantly more likely to test positive for cannabis use than those in states without such laws. State medical cannabis laws had little impact on the odds of cannabis positivity among fatally injured drivers.
Extracting value from total-body PET/CT image data - the emerging role of artificial intelligence
Authors: Shiyam Sundar Lalith Kumar , Gutschmayer Sebastian , Maenle Marcel , Beyer Thomas 
Published: April 2024

AbstractThe evolution of Positron Emission Tomography (PET), culminating in the Total-Body PET (TB-PET) system, represents a paradigm shift in medical imaging. This paper explores the transformative role of Artificial Intelligence (AI) in enhancing clinical and research applications of TB-PET imaging. Clinically, TB-PET’s superior sensitivity facilitates rapid imaging, low-dose imaging protocols, improved diagnostic capabilities and higher patient comfort. In research, TB-PET shows promise in studying systemic interactions and enhancing our understanding of human physiology and pathophysiology. In parallel, AI’s integration into PET imaging workflows—spanning from image acquisition to data analysis—marks a significant development in nuclear medicine. This review delves into the current and potential roles of AI in augmenting TB-PET/CT’s functionality and utility. We explore how AI can streamline current PET imaging processes and pioneer new applications, thereby maximising the technology’s capabilities. The discussion also addresses necessary steps and considerations for effectively integrating AI into TB-PET/CT research and clinical practice. The paper highlights AI’s role in enhancing TB-PET’s efficiency and addresses the challenges posed by TB-PET’s increased complexity. In conclusion, this exploration emphasises the need for a collaborative approach in the field of medical imaging. We advocate for shared resources and open-source initiatives as crucial steps towards harnessing the full potential of the AI/TB-PET synergy. This collaborative effort is essential for revolutionising medical imaging, ultimately leading to significant advancements in patient care and medical research.
The implications of single-cell RNA-seq analysis in prostate cancer: unraveling tumor heterogeneity, therapeutic implications and pathways towards personalized therapy
Authors: Feng De-Chao , Zhu Wei-Zhen , Wang Jie , Li Deng-Xiong , Shi Xu , Xiong Qiao , You Jia , Han Ping , Qiu Shi , Wei Qiang , Yang Lu 
Published: April 2024

AbstractIn recent years, advancements in single-cell and spatial transcriptomics, which are highly regarded developments in the current era, particularly the emerging integration of single-cell and spatiotemporal transcriptomics, have enabled a detailed molecular comprehension of the complex regulation of cell fate. The insights obtained from these methodologies are anticipated to significantly contribute to the development of personalized medicine. Currently, single-cell technology is less frequently utilized for prostate cancer compared with other types of tumors. Starting from the perspective of RNA sequencing technology, this review outlined the significance of single-cell RNA sequencing (scRNA-seq) in prostate cancer research, encompassing preclinical medicine and clinical applications. We summarize the differences between mouse and human prostate cancer as revealed by scRNA-seq studies, as well as a combination of multi-omics methods involving scRNA-seq to highlight the key molecular targets for the diagnosis, treatment, and drug resistance characteristics of prostate cancer. These studies are expected to provide novel insights for the development of immunotherapy and other innovative treatment strategies for castration-resistant prostate cancer. Furthermore, we explore the potential clinical applications stemming from other single-cell technologies in this review, paving the way for future research in precision medicine.
Retrospective case series of high-density silicone oil (Oxane HD) in severe proliferative vitreorretinal retinal detachment patients
Authors: Oliveira Ramon Antunes De , Pesquero Vinicius Oliveira , Ribeiro Lucas Zago , Polizelli Murilo Ubukata , Silva Aalec Rinhel Souza Ferreira Da , Moraes Nilva Simeren Bueno De , Fernandes Rodrigo Antonio Brant , Junior Octaviano Magalhaes , Maia Mauricio 
Published: April 2024

Abstract
Background
Describe complications and clinical outcomes of heavy silicone oil (HSO) Oxane HD® use as an alternative to overcome the challenges of performing vitrectomy to treat tractional and rhegmatogenous retinal detachments with proliferative vitreoretinopathy (PVR).

Methods
A retrospective, observational study was performed on patients from one center from August 2014 to Aug 2023. It was included patients who underwent surgery using HSO Oxane HD® to treat rhegmatogenous retinal detachment with PVR or mixed tractional and rhegmatogenous diabetic retinal detachment. Severely ill patients who could not attend to follow up were excluded. The primary outcome was successful retinal attachment at first postoperative month. A descriptive analysis was performed.

Results
Among the 31 patients, 29 (93.5%) underwent surgeries due to rhegmatogenous retinal detachment and two (6.5%) for diabetic retinal detachment. The primary anatomic success was achieved in 27 (87.1%) patients. At the final visit, 17 (56.6%) had vision better than 20/400 (range, 20/30 to light perception). The vision was stable or improved in 22 (76.8%) patients at the end of follow-up. Nineteen (61.3%) patients required hypotensive eye drops after HSO use and twelve (38.7%) still required hypotensive eye drops at the final follow-up; three (9.7%) patients required additional glaucoma surgeries.

Conclusions
HSO is safe and useful for complex retinal detachments cases specially with inferior tears and PVR. Ocular hypertension is frequent and usually clinically controlled with hypotensive eyedrops. Close postoperatively follow-up is advised due to the ocular complications, particularly elevated intraocular pressure and emulsification.
Anesthesia management of laparoscopic right colectomy in an older patient with postoperative tetralogy of Fallot with residual anomaly
Authors: Mori Satori , Ito Hisakatsu , Sugimoto Sadamu , Hibi Daisuke , Kameyama Akiyo , Kawakami Masaaki , Takazawa Tomonori 
Published: April 2024

Abstract
Background
Diversity in hemodynamics of adult congenital heart disease necessitates a case-by-case selection of appropriate surgical and anesthetic options. However, previous case reports regarding the management of laparoscopic surgery in adult patients with congenital heart disease are limited.

Case presentation
A 72-year-old man who underwent a laparoscopic right colectomy for colon cancer had a residual ventricular septal defect and right ventricular outflow tract obstruction despite post-repair of tetralogy of Fallot. Pulmonary hypertension or right ventricular dysfunction was not observed. The preoperative pulmonary to systemic blood flow ratio (Qp/Qs) was 2.3. After positive pressure ventilation and insufflation, the amount of left-to-right ventricular shunting decreased, and the Qp/Qs approached 1.0, as calculated from pulmonary arterial and systemic arterial blood gas analysis.

Conclusions
Laparoscopic surgery might be tolerable in patients with tetralogy of Fallot who have preserved the right ventricular function, left-to-right ventricular shunting, and no high pulmonary vascular resistance.
Occipital abscess developed after endovascular AVM treatment with liquid embolizing agent: case report
Authors: Yuksek Muhammed Erkam , Gul Busra , Kenan Mehmet , Erdi Mehmet Fatih , Yıldırım Seyfullah , Guney Ahmet Onder , Keskin Fatih 
Published: April 2024

Abstract
Background
In arteriovenous malformations, endovascular embolization was first used in the 1960s to occlude feeding vessels. In recent years, the success of endovascular treatments has increased. Thus, the use of endovascular therapy in the treatment of arteriovenous malformation has become widespread. Recently, it is the primary treatment method or an adjunctive treatment to surgery. The development of intracranial abscess after endovascular treatment with a liquid embolizing agent has been reported very rarely in the literature. In this article, a case of intracerebral abscess that developed after endovascular treatment with a liquid embolizing agent was presented.

Case Presentation
A 24-year-old male patient was admitted to the hospital with the complaints of dizziness and syncope. Arteriovenous malformation was observed in radiological imaging. Endovascular treatment for the AVM was performed. He was admitted to the hospital with similar complaints 2 months after the treatment. An abscess was observed around the AVM in the patient's cranial imaging. Infected materials in the mall were surgically removed, and the abscess was drained. After antibiotic treatment, the patient was discharged with full recovery.

Conclusion
Due to the recent popularity of endovascular treatment methods, the incidence of abscess formation after embolization may change in the near future. Further research should be done to prevent this serious complication.
Outcome associated with use of winged expandable titanium cage following cervical corpectomy: an institutional experience
Authors: Prakash Anand , Bharti Rohit , Chauhan Ganesh , Dutta Gautam , Sahay Chandra Bhushan 
Published: April 2024

Abstract
Background
In patients with spinal cord compression behind the cervical vertebra who presented with myelopathy, one of the treatment modalities is anterior cervical corpectomy followed by insertion of graft or implant. Autogenous bone graft has been the choice of implant for corpectomy in the past, but due to donor site complications, it has been majorly replaced with other implants like titanium made expandable and non-expandable cages. Studies on titanium made implants for cervical compressive myelopathy have mostly focused on expandable cages with separate plates. Studies on hybrid cages and winged expandable titanium cages with (WETC) are lacking, especially in patients with poor Nurick grade. Here, we present clinical outcomes and side effects of WETC use following cervical corpectomy in 81 participants from a tertiary care center from Eastern India with 6 months of follow-up.

Results
We observed a considerable improvement in clinical outcomes which was measured using Nurick grade as mean scores changed from 4.06 ± 0.85 during the pre-operative stage to 2.85 ± 1.16 post-operation (P < 0.05). There was also a considerable improvement in the pain status as in the pre-operative stage there were three patients with mild pain, 53 with moderate and 25 with severe pain, but post-operation there were 53 patients with mild pain, only two with moderate pain and none with severe pain (P < 0.00001). In these participants, we observed that post-surgical Nurick grade was not associated with age, gender or time since first symptoms but was strongly associated with pre-surgical Nurick grade at P < 0.05.

Conclusion
With WETC (in situ plate), we were able to obtain good outcomes with less serious complication in patients with poor Nurick grade. As size of implant is prefixed and can be expanded in WETC, it takes relatively less time to insert, thus leading to shorter operative time. With good endplate preparation and avoiding over expansion of cage, WETC avoids complications.
Ultrasonographic and power doppler parameters of nails fail to differentiate between onychodystrophy in patients with psoriasis vulgaris or psoriatic arthritis
Authors: Tanaka Anber Ancel , Werner Betina , Bragatto Annelise Correa Bueno , Skare Thelma Larocca , Stadler Bárbara 
Published: April 2024

Abstract
Background
Nail involvement is frequent in patients with psoriasis (Pso) and psoriatic arthritis (PsA) and there is a relationship between nail involvement and inflammation of the enthesis. The main objective of the present study is to describe the ultrasound findings and clinical characteristics of nails from patients with psoriasis and psoriatic arthritis with and without nail dystrophy.

Methods
A cross-sectional study including consecutive patients with PsO and PsA was carried out. The study patients were divided into 4 groups, totaling 120 participants. Group 1: patients with psoriasis vulgaris and clinically normal nails; Group 2: patients with psoriasis vulgaris and onychodystrophy; Group 3: patients with psoriatic arthritis and clinically normal nails; Group 4: patients with psoriatic arthritis and onychodystrophy; All patients were submitted to dermatological and rheumatological clinical analysis. Ultrasound examinations was performed by a single examiner, blinded to all clinical data, with ultrasound high resolution, in B-mode or gray-scale (GS), Power Doppler (PD) and Spectral Doppler.

Results
A significant difference was found between the groups regarding the variable Psoriasis Area and Severity Index (PASI) (p = 0.008) and body surface area (BSA) (p = 0.005), with patients with psoriatic arthritis having lower PASI and BSA compared to patients with only cutaneous psoriasis. A positive relationship was found with the average ultrasound thickness of the nail bed and the Nail Psoriasis Severity Index (NAPSI) in correlation analysis (rho = 0.344). When we grouped patients with psoriasis and psoriatic arthritis, there was no significant difference between the cutaneous psoriasis groups and the psoriatic arthritis groups in terms of nail plate GS (p = 0.442), nail bed PD (p = 0.124).

Conclusion
Greater nail bed thickness indicates early psoriatic nail disease, as confirmed in our study correlating NAPSI with nail bed thickness. Ultrasonography is a low-cost exam, promising in the evaluation, showing that the ultrasound grayscale is consistent with those who have dystrophic nails, but it can’t distinguish psoriasis from psoriatic arthritis, even in those with nail dystrophy.
Yields, growth and water use under chemical topping in relations to row configuration and plant density in drip-irrigated cotton
Authors: Wang Xuejiao , Hu Yanping , Ji Chunrong , Chen Yongfan , Sun Shuai , Zhang Zeshan , Zhang Yutong , Wang Sen , Yang Mingfeng , Ji Fen , Guo Yanyun , Li Jie , Zhang Lizhen 
Published: April 2024

Abstract
Background
Water deficit is an important problem in agricultural production in arid regions. With the advent of wholly mechanized technology for cotton planting in Xinjiang, it is important to determine which planting mode could achieve high yield, fiber quality and water use efficiency (WUE). This study aimed to explore if chemical topping affected cotton yield, quality and water use in relation to row configuration and plant densities.

Results
Experiments were carried out in Xinjiang China, in 2020 and 2021 with two topping method, manual topping and chemical topping, two plant densities, low and high, and two row configurations, i.e., 76 cm equal rows and 10+66 cm narrow-wide rows, which were commonly applied in matching harvest machine. Chemical topping increased seed cotton yield, but did not affect cotton fiber quality comparing to traditional manual topping. Under equal row spacing, the WUE in higher density was 62.4% higher than in the lower one. However, under narrow-wide row spacing, the WUE in lower density was 53.3% higher than in higher one (farmers’ practice). For machine-harvest cotton in Xinjiang, the optimal row configuration and plant density for chemical topping was narrow-wide rows with 15 plants m-2 or equal rows with 18 plants m-2.

Conclusion
The plant density recommended in narrow-wide rows was less than farmers’ practice and the density in equal rows was moderate with local practice. Our results provide new knowledge on optimizing agronomic managements of machine-harvested cotton for both high yield and water efficient.
Chronic cough in children: an evidence-based clinical practice guideline adapted for the use in Egypt using ‘Adapted ADAPTE’
Authors: Fouda Eman Mahmoud , Shaaban Hala Hamdi , Elattar Mona Mohsen , Mostafa Abla Saleh , Hamed Dina H. , Saleh Aya Samir Mohamed , Ishak Sally Raafat , Hamed Tarek , Mohamed Magda Hassab Allah , Hussien Shahenaz Mohamoud , Rashad Mohamed Mahmoud , Elnady Hala Gouda , Metwally Hoda M. Salah El Din , Elghaffar Laila Abd , Reda Sherif , Abdel Baky Ashraf , Omar Tarek , Amer Yasser , Sarhan Dina Tawfeek , Refay Amira S. El ,   On behalf of the Egyptian Pediatric Clinical Practice Guidelines Committee (EPG)
Published: April 2024

Abstract
Background
We recently adopted a guideline for chronic cough in children in the Egyptian health system. Adapting clinical practice guidelines (CPGs) to the local healthcare setting is a valid alternative to de-novo development that can improve their uptake and implementation without demanding a substantial drain on resources. The objective of this study was to adapt evidence-based recommendations from global high-quality CPGs for children with a chronic cough to suit the Egyptian healthcare context.

Methods
We followed the Adapted ADAPTE methodological framework for guideline adaptation. This process includes three phases: set-up, adaptation, and finalization. A guideline adaptation group (GAG) and an external review group including clinical content experts and methodologists conducted the process.

Results
The GAG adapted 10 sections of recommendations from three original CPG(s) including (i) the American College of Chest Physicians (ACCP) 2006–2020, (ii) the European Respiratory Society (ERS) 2019, (iii) the Korean Academy of Asthma, Allergy and Clinical Immunology (KAAACI) 2018. A set of CPG implementation tools was added to enhance implementability including an algorithm, a slide presentation for clinical diagnosis, investigations and treatment of chronic cough, patient education, and online resources.

Conclusion
The adapted CPG provides pediatricians and related healthcare workers with applicable evidence-based recommendations for chronic cough in children in Egypt. The project also highlighted the utility of Adapted ADAPTE and the invaluable collaboration between the clinical and methodological experts for the adaptation of pediatric national guidelines.