bluuebunny/crossref_metadata_2025_split huggingface dataset:
Undifferentiated small‐cell carcinoma of the urinary bladder: Report of two cases with a primary urinary cytodiagnosis
Authors: Borghi Laura , Bianchini Enzo , Altavilla Giuseppe 
Published: February 2007

AbstractTwo undifferentiated small‐cell carcinomas of the urinary bladder are reported. The patients, 68‐ and 55‐yr‐old men, respectively, presented with painless hematuria. In the first case, numerous small, lymphocyte‐like cells with coarse chromatin, sometimes with small nucleoli, and high nuclear/cytoplasmatic ratios were found in cytologic urine specimens. A cytodiagnosis of undifferentiated small‐cell cancer was made. In the second case, urine samples showed rare aggregates of small, undifferentiated cells in association with malignant urothelial cells. The cytodiagnosis of mixed tumor composed of undifferentiated small cell and transitional carcinoma was confirmed by histologic examination. The presence of focal reactivity with anti‐chromogranin antibody and neurosecretory granules via electron microscopy supports a neuroendocrine differentiation for the small neoplastic cells. The patients died 13 and 8 mo after diagnosis, respectively. © 1995 Wiley‐Liss, Inc.
Multilocular renal cyst: A diagnostic pitfall on fine‐needle aspiration cytology: Case report
Authors: Morgan Claire , Greenberg Merle L. 
Published: February 2007

AbstractBenign renal lesions, apart from simple cysts, are rarely sampled by fine‐needle aspiration biopsy (FNAB) and are potential diagnostic pitfalls. A complex renal mass in a 33‐yr‐old pregnant woman, presenting in the second trimester with haematuria, was aspirated twice, a week apart, under ultrasound guidance. The second FNAB yielded predominantly mesenchymal elements thought to represent an angiomyolipoma, but the mass was identified as a multilocular renal cyst (MLRC) on the nephrectomy specimen. Differential diagnoses of angiomyolipoma, MLRC, and renal cell carcinoma (RCC) are compared and discussed in relation to patient management. © 1995 Wiley‐Liss, Inc.
Application of immunostaining for muscle specific actin in detection of myoepithelial cells in breast fine‐needle aspirates
Authors: Masood Shahla , Lu Leo , Assaf‐Munasifi Manal (Nola) , McCaulley Karen 
Published: February 2007

AbstractMyoepithelial cells play an important role in the interpretation of breast fine‐needle aspiration biopsy, since these cells are believed to be a component of a benign process in breast lesions. Myoepithelial cells are usually easy to recognize, however, other cells can morphologically simulate myoepithelial cells and make the distinction difficult. To assess the feasibility of immunostaining as an adjunct to identify myoepithelial cells, we have used immunocytochemistry using monoclonal antibody against muscle specific actin (MSA) in our breast fine‐needle aspirates. Herein, we report our experience in immunocytochemical detection of myoepithelial cells using labeled Streptavidin Biotin Detection System on destained Papanicolaou stained smears and cell block preparations obtained from breast aspirates. © 1995 Wiley‐Liss, Inc.
Chronic radiation effects: A correlative study of smears and biopsies from the cervix and vagina
Authors: Shield Paul W. 
Published: February 2007

AbstractCervicovaginal smears and biopsies from patients treated with radiotherapy for cervical carcinoma were examined morphologically and immunochemically to provide information on the tissue derivation of cells characteristic of chronic radiation effect in postirradiation smears.In biopsies, stromal changes, such as fibrosis, vessel changes, and atypical fibroblasts were most common. Ulceration, leucocytic infiltration, multinucleated giant cells, regenerative epithelium, and atypical glandular epithelial cells were also present in some specimens. These changes were reflected in smears collected from the same patients, where multinucleated giant cells, repair cells, and large atypical cells were often present. Correlation of smears and biopsies suggest that repair cells are collected from areas of epithelial regeneration and glandular radiation atypia. Sampling of ulcerative or eroded tissue may produce smears with multinucleated giant cells, atypical stromal cells, endothelial cells, and numerous macrophages. Correct recognition of these cell types and smear patterns may assist in avoiding false positive diagnoses. © 1995 Wiley‐Liss, Inc.
Cytodiagnosis of parvoviruS b19 infection from ascites fluid of hydrops fetalis: Report of a case
Authors: Iwa Nobuzo , Yutani Chikao 
Published: February 2007

AbstractThe cytologic changes in the smears of fetus ascites fluid with parvovirus B19 infections are described. Cytology revealed the ground‐glass appearance of the nuclei having a perinuclear halo, and the nuclear chromatin was spread as a thin rim around the inclusion. Subsequently, monoclonal antibodies for parvovirus B19 were applied to identify the specific antigen in the Papanicolaou‐destained specimens. Positive staining reaction products were found throughout the cytoplasm of cells. Similar phenomena were confirmed in paraffin‐embedded tissue sections of many organs in autopsy materials. It is hoped that recognition of those virus‐infected cells in the body cavity fluid of the fetus will be helpful in making a diagnosis of hydrops fetalis associated with parvovirus B19 infections of the pregnant woman. © 1995 Wiley‐Liss, Inc.
Severe eosinophilia and hepatocellular carcinoma: An unusual association
Authors: Yuen Benjamin H. , Reyes Cesar V. , Rawal Paresh A. , Sosman Jeffrey , Jensen Joanne 
Published: February 2007

AbstractPeripheral eosinophilia is an unusual but recognized paraneoplastic manifestation of malignant diseases. We report a case of eosinophilia associated with hepatocellullar carcinoma which is the second case described in English literature. © 1995 Wiley‐Liss, Inc.
Diagnosis of Epstein‐Barr virus associated nasopharyngeal carcinoma using fine‐needle aspiration biopsy and molecular diagnostics
Authors: Smith Stuart S. , Fowler Larry J. , Hausenfluke Linda , Cho Chong G. , Eagan Phyllis A. , Gulley Margaret L. 
Published: February 2007

AbstractMolecular technology is being utilized increasingly for diagnostic purposes by practicing pathologists. Techniques such as Southern blot, in situ hybridization, and polymerase chain reaction have recently been introduced to the clinical laboratory setting. We describe a case of nasopharyngeal carcinoma that highlights the potential utility of DNA technology to secure an accurate diagnosis of a fine‐needle aspiration biopsy. In this patient, cytologic examination of a cervical lymph node aspirate strongly suggested the possibility of a nasopharyngeal carcinoma. Needle aspirate material was submitted for molecular genetic detection of the Epstein‐Barr virus (EBV) genome. Nine micrograms of DNA were isolated, and the presence of clonal EBV DNA was detected by the Southern blot technique. The presence of clonal EBV supported the cytologic diagnosis of nasopharyngeal carcinoma. Subsequent biopsy of a nasopharyngeal mass revealed undifferentiated carcinoma, and in situ hybridization revealed that EBV was restricted to the malignant epithelial cells. This case illustrates how molecular technology can provide new information that is useful in diagnostic cytopathology. © 1995 Wiley‐Liss, Inc.
Ultrafast papanicolaou stain is not limited to rapid assessments: Application to permanent fine‐needle aspiration smears
Authors: Yang Grace C. H. 
Published: February 2007

AbstractUltrafast Papanicolaou (Pap) stain, a 90‐second preparation originally designed for the immediate assessment of fine‐needle aspiration (FNA) smears (Yang and Alvarez, Acta Cytol 1995;39:55–60), can also be adapted for permanent FNA smears. It involves the addition of three simple steps prior to the conventional Pap procedure: the first step is to make the cells appear larger, thus increasing the resolution for analysis of cellular details; the second step is to hemolyse the background blood, thus unmasking tumor cells; and the third step is to bring out the vibrant colors in the cells and the nucleoli, which stain red. © 1995 Wiley‐Liss, Inc.
Atypical endocervical glandular cells: Accuracy of cytologic diagnosis
Authors: Lee Kenneth R. , Manna Edward A. , St. John Timothy 
Published: February 2007

AbstractAtypical cells thought to be of endocervical glandular origin often cause diagnostic uncertainty in cervicovaginal smears. For this reason consecutive cases of endocervical glandular atypia diagnosed in smears were correlated with subsequent biopsy diagnoses and then retrospectively reviewed. Smears were originally diagnosed as “mild glandular atypia, probably reactive” or “severe glandular atypia, suggestive of adenocarcinoma in situ” (AIS). Biopsy follow‐up was obtained on 34 of 58 patients diagnosed with severe endocervical glandular atypia. Nine patients (26%) had AIS, three with concomitant high‐grade squamous intraepithelial lesions (HSIL) and two with invasive adenocarcinoma. Eighteen patients (53%) had HSIL only. Seven had benign changes. Of 152 patients diagnosed with mild glandular atypia, biopsy follow‐up was obtained on 40. One patient had AIS; 14 (35%) had HSIL; one had low‐grade SIL (LSIL); and 24 (60%) had benign changes.Blinded review of these smears yielded results similar to those in the biopsy follow‐up, that is, the prediction of AIS on smears included most cases of AIS, some invasive adenocarcinomas, a significant number of HSIL cases and a few benign lesions. A review diagnosis of “reactive glandular cells” proved to be HSIL in 31% of cases and AIS in one case.We conclude that patients with a diagnosis of severe glandular atypia in smears may prove to have AIS or invasive adenocarcinoma, but often have HSIL without concomitant AIS. In addition, although “reactive” glandular atypia in smears usually reflects a benign condition, a significant minority of such patients prove to have HSIL. © 1995 Wiley‐Liss, Inc.
Metastases to the thyroid gland: Diagnosis by aspiration cytology
Authors: Michelow Pamela M. , Leiman Gladwyn 
Published: February 2007

AbstractThe purpose of this study was to document the incidence, nature and source of neoplasms metastatic to the thyroid gland, which were diagnosed by fine needle aspiration (FNA) cytology. In the seven year period from 1986 to 1992, 21 cases were identified with metastatic malignancies in FNA specimens from the thyroid. This represented 7.5% of neoplastic thyroid lesions aspirated in this unit. All patients presented clinically with thyromegaly or discrete nodules. Only five patients were known to have malignancies of other sites prior to FNA. The majority of metastatic nodules were bronchogenic in origin (nine). The gastrointestinal tract (five) and melanomas (two) were the next most frequent sources in the series. Single cases arose in the prostate, larynx, kidney (all carcinomas), and uterus (a leiomyosarcoma). One patient had a thyroid deposit of acute myeloblastic leukemia. This large study demonstrated that tumors of many histological types may involve the thyroid gland, and furthermore, may masquerade as primary thyroid malignancies. Recognition of an alien cell type not only prevents inappropriate thyroid surgery, but may also direct the search for the unsuspected or unknown primary. Metastases to the thyroid gland occur more frequently than is generally appreciated. FNA is the procedure of choice for evaluation of thyroid nodules in general, and thyroid metastases in particular. © 1995 Wiley‐Liss, Inc.
Detached single cilia: Another potential pseudomicrobe seen in bronchoalveolar lavage specimens
Authors: Stanley Michael W. , Mrak Robert E. , Bardales Ricardo H. 
Published: February 2007

AbstractHigh magnification examination of Romanovsky‐stained bronchoalveolar lavage (BAL) material may be needed, if one is to confidently exclude small organisms such as toxoplasmosis, histoplasmosis, and the negative images of mycobacteria. Pseudomicrobes have been described, and detailed criteria must be carefully applied, if incorrect diagnoses are to be avoided. We recently noted numerous free‐lying, uniform, eosinophilic, straight or slightly curved bacilliform structures in such a specimen. These were seen on Diff‐Quik®‐stained material, but were extremely pale on Papanicolaou‐stained slides. Comparison with the cilia of contaminating bronchial cells showed these pseudomicrobes to have similar length, diameter, and tinctorial properties. Electron microscopy of the sedimented cell pellet showed isolated, detached single cilia (DSC) far removed from any bronchial cells. Twenty BAL's from 13 males and seven females (median age = 48.5) undergoing infection surveillance were then retrospectively reviewed. DSC were present in 16 specimens (80%), but were rare in three of these. Although present in cases with prominent bronchial cell contamination (n=6 cases), DSC were also encountered when ciliated cells were rare (n=13), or absent (n=1). Familiarity with these distinctive structures should make it unlikely that they will be mistaken for infectious organisms. © 1995 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology of ductal adenoma: Report of a case associated with a mucocele‐like lesion
Authors: Mesonero Clara E. , Tabbara Sana 
Published: February 2007

AbstractDuctal adenoma (DA) is an uncommon breast lesion that can histologically and clinically mimic carcinoma. We performed a fine‐needle aspiration (FNA) of a DA. Cytologically, the lesion had features overlapping with those of mucinous carcinoma (MC), mucocele‐like lesion, lactating adenoma (LA), and in retrospect with intraductal papilloma (IP). The smears were highly cellular and contained numerous monolayered sheets of ductal cells with prominent punched‐out, small vacuoles distending the cytoplasm. The nuclei were mostly round to oval and had bland chromatin. Occasionally cells with enlarged nuclei and conspicuous nucleoli were present. The background showed large mucin pools, scattered single cells with mild nuclear atypia, some with apocrine metaplasia, rare stripped nuclei, and a fibrovascular stromal component. Calcifications were also present.We compare our cytologic findings with those of the lesions considered in the differential diagnosis. Due to its rare incidence and unusual features, DA may represent a diagnostic pitfall on FNA. Increased awareness of its cytologic appearance may help prevent a misdiagnosis. © 1995 Wiley‐Liss, Inc.
Peritoneal implantation of meningeal melanosis via ventriculoperitoneal shunt: A case report and review of the literature
Authors: Gattuso Paolo , Carson Henry J. , Attal Houssam , Castelli Melanie J. 
Published: February 2007

AbstractSystemic spread of primary intracranial neoplasms is rare and may be due to ventriculoperitoneal shunt (VPS). The most common tumors to metastasize via VPS are germinoma of the pineal gland and medulloblastoma. We report a case of 16‐yr‐old girl with central nervous system malignant melanosis who developed subsequent peritoneal implants via VPS. To the best of our knowledge, this patient represents the third reported case of meningeal melanosis or melanoma which metastasized to the peritoneal cavity via VPS. The VPS should be considered as possible mode of systemic spread in patients with primary cranial malignancy. © 1995 Wiley‐Liss, Inc.
Fine‐needle aspiration cytology in Kimura's disease
Authors: Jayaram Gita , Peh Kaik Boo 
Published: February 2007

AbstractThree patients presenting with parotid, submandibular, and/or lymph node masses were subjected to fine‐needle aspiration cytology. Smears showed dissociated and clustered endothelial cells, eosinophils, lymphocytes, and Warthin Finkeldey giant cells. In two cases a diagnosis of Kimura's disease was suggested from the FNA cytologic smears. In the third case the presence of mononucleate cells with prominent nucleoli led to a suspicion of Hodgkin's disease. Excision biopsy and histopathologic study established a diagnosis of Kimura's disease in all three cases. © 1995 Wiley‐Liss, Inc.
Intracystic papillary carcinoma of the female breast with secretory activity: The significance of aspiration cytology as diagnostic procedure
Authors: Kyriazis Andreas P. , Kyriazis Aikaterini A. 
Published: February 2007

AbstractWe report the case of an intracystic papillary carcinoma of the breast in an elderly woman. Gross and microscopic observations support the contention that intracystic fluid is partly a result of secretory activity by the neoplastic epithelial cells and that, intracystic hemorrhage, which may contribute to it, is a secondary event complicating the primary process. Cytology of the aspirated fluid was negative for malignant cells. It is concluded that in the case of large cystic breast lesions, aspiration cytology may not be helpful in establishing the malignant nature of the lesion and that a negative cytology should be interpreted with caution, always taking under consideration the clinical picture of the disease. A concomitant adenocarcinoma was also present in the opposite breast, the second case reported in the literature. © 1995 Wiley‐Liss, Inc.
Modified staining technique for the on‐site evaluation of fine‐needle aspiration specimens from fatty tissues
Authors: Logan Lisa S. , Gupta Prabodh K. 
Published: February 2007

AbstractThe adequacy of on‐site evaluation of fine‐needle aspiration from fatty tissues can be complicated by less than optimal adherence or fixation of the aspirated cells to the surface of the glass slide. This article describes a modifified staining technique that optimizes the recovery of material in such circumstances. 1995;13:345–346. © 1995 Wiley‐Liss, Inc.
Estimation of estrogen receptor content in fine‐needle aspirates from breast cancer using the monoclonal antibody 1d5 and microwave oven processing: Correlation with paraffin embedded and frozen sections determinations
Authors: Schmitt Fernando Carlos , Bento Maria José , Amendoeira Isabel 
Published: February 2007

AbstractWe describe a method of immunocytochemically assessing estrogen receptor (ER) status on alcohol‐fixed smears obtained by fine‐needle aspiration (FNA) from breast cancer patients, using a commercially available monoclonal antibody (1D5) with microwave oven processing. A series of 31 cases of aspirates from breast cancer were analysed and the results were compared with assessment by ER immunocytochemical assay using the same procedure on formalin‐fixed tissue and with assessment by ER‐ICA assay on frozen sections. The results were scored semiquantitatively using a five grade scoring system. Of the 31 cases examined, 21 were positive at least by two methods and 10 were negative for all three determinations. The results obtained in the ER immunocytochemical assay on aspirates and paraffin‐sections using the antibody 1D5 and those obtained on frozen sections using the antibody H222 were closely similar. In only one case was it not possible to interpret the reaction in the cytological specimen because there was a strong background in the smear. In general, we obtained more intense positivity with the antibody 1D5 in aspirates and formalin‐fixed material than with the antibody H222 in frozen sections. The scoring results of the three methods were almost identical. We conclude that the application of ER method on alcohol‐fixed smears will eliminate the need for using a special fixation procedure and will provide several advantages, such as: improvement in morphological concomitant analysis, utilization whenever malignancy is found without necessity to re‐aspirate the patient, and adequacy of archival material. © 1995 Wiley‐Liss, Inc.
Endocervical glandular atypia: A “new” problem for the cytologist
Authors: Wilbur David C. 
Published: February 2007

AbstractNew patterns of cellular presentation on cervical smears have become more frequent since the introduction of new sampling devices which provide increased material from the upper portions of the endocervical canal. The normal histologic variability of the endocervical canal, as well as the presence of tubal metaplasia, infectious and inflammatory reactions, squamous lesions involving endocervical glands, and true endocervical neoplastic lesions in these areas, have presented the cytologist with a variety of cellular appearances which may cause difficulties in differential diagnosis.Recognition of these entities, their cytologic manifestations, and the effects of increased high endocervical sampling, will allow the cytologist to begin the process of gaining the experience necessary to assess these new cellular patterns. A potential management protocol for patient follow‐up is presented which will allow the user to investigate such cases in an efficient and cost effective manner. © 1995 Wiley‐Liss, Inc.
Quantitative volumetric analysis of cardiac morphogenesis assessed through micro‐computed tomography
Authors: Butcher Jonathan T. , Sedmera David , Guldberg Robert E. , Markwald Roger R. 
Published: February 2007

AbstractD renderings of embryonic chick hearts at different stages of development (HH16 ‐ HH36) using Micro‐CT. All images are at the same scale. See Butcher et al., Developmental Dynamics 236:803–810.
Structural Distortion and Magnetic Behavior in Cyanide‐Bridged FeIII2NiII2 Complexes
Authors: Li Dongfeng , Clérac Rodolphe , Wang Guangbin , Yee Gordon T. , Holmes Stephen M. 
Published: February 2007

AbstractThe X‐ray structure, spectroscopic, and magnetic properties of a cyanide‐bridged complex, {[(Tp*)FeIII(CN)3]2[NiII(bipy)2]2[OTf]2}·2H2O (2) are described [Tp* = hydridotris(3,5‐dimethylpyrazol‐1‐yl)borate; bipy = 2,2′‐bipyridine; OTf = trifluromethanesulfonate]. Magnetic measurements indicate that 2 exhibits an S = 3 ground state and slow relaxation of the magnetization. Magnetostructural comparisons to the more symmetrical analog {[(Tp*)FeIII(CN)3]2[NiII(DMF)4]2[OTf]2}·2DMF (1) are also described. Surprisingly, the magnetic properties of 1 and 2 are similar despite the presence of a non‐planar {FeIII2NiII2(μ‐CN)4} core and severely distorted NiII coordination sphere in 2, suggesting that the NiII centers play a minor role in the slow magnetization relaxation behavior of the clusters. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Systematic Counterion Tuning of the Solid‐State Structure of [Pt(thiourea)4]2+
Authors: Schiessl Wolfgang , Puchta Ralph , Bugarčić Živadin D. , Heinemann Frank W. , van Eldik Rudi 
Published: February 2007

AbstractFour different limiting molecular conformations are possible for the homoleptic square‐planar complex tetrakis(thiourea)platinum(II) depending on the up‐down orientation of the four thiourea (TU) ligands with respect to the PtS4 plane. The realization of all four possible conformers can be achieved by a systematic variation of the counter anion making use of their individual hydrogen bonding ability. In total, the molecular and crystal structures of seven tetrakis(thiourea)platinum(II) complexes were determined by X‐ray diffraction. These are: [Pt(TU)4]Cl2, [Pt(TU)4]I2, [Pt(TU)4](CF3SO3)2, [Pt(TU)4](BPh4)2, [Pt(TU)4](ClO4)2, [Pt(TU)4]SiF6·0.25H2O and [Pt(TU)4]S2O6. In all cases thiourea is coordinated by the sulfur atom and the two amino groups are not involved in the complex formation. The four independent Pt–S distances do not differ significantly from each other with an average value of 2.324(3) Å. The coordination geometry around the platinum center is distorted square‐planar. The orientation of the four TU groups in [Pt(TU)4]2+ depends on the molecular packing forces and hydrogen bonding ability of the counter anion. Hydrogen bonds of the type N–H···X (X = Cl–, I–, F–, O and S) are observed.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)