bluuebunny/crossref_metadata_2025_split huggingface dataset:
ОПЕРАЦІЯ «ФРІДЕРІКУС-1» ЯК ПЕРЕДУМОВА ПРОВЕДЕННЯ НАСТУПАЛЬНОЇ ОПЕРАЦІЇ «БЛАУ». ПІДГОТОВКА ДО ЛІКВІДАЦІЇ БАРВІНКІВСЬКОГО ПЛАЦДАРМУ
Authors: Кузнєцов Михайло Олексійович 
Published: April 2024

Друга світова війна, як і багато інших війн в історії людства, супроводжувалась людськими жертвами, втратою озброєння та військової техніки, зруйнованими об’єктами цивільної та військової інфраструктури. Велике значення для зменшення цих втрат та руйнувань як в роки Другої світової війни, так і в сучасній російсько-українській, має якісне планування та проведення військових операцій. Одними з багатьох таких операцій, проведених у ході Другої світової війни, є операції на Харківському напрямку у травні 1942 року: Харківська наступальна операція радянських військ та операція «Фрідерікус-1» військ гітлерівської коаліції. Операція «Фрідерікус-1» була операцією з ліквідації Барвінківського плацдарму, зайнятого РСЧА, та передувала літньому наступу вермахту на Кавказ та Сталінград у 1942 році та яка в кінцевому результаті мала призвести до знищення РСЧА та Радянського союзу в цілому, реалізації ідеї Гітлера щодо життєвого простору на сході та захоплення нових територій і земель для їх колонізації.
У статті висвітлено загальний план операції, процес планування, проблемні питання, які виникали під час підготовки до її проведення, такі як матеріально-технічне забезпечення військ гітлерівської коаліції, ліквідація опору партизан та представників національно-визвольного руху на окупованій території України, їх вирішення та подолання. З’ясовано склад німецьких, румунських та угорських військ, які безпосередньо готувались до участі в операції або відігравали допоміжну роль, їх місце у проведенні наступу. У праці використано архівні та публіцистичні матеріали, мемуари, документи вермахту, присвячені даній проблематиці. Наголошено на питаннях якісного планування операції та чинниках, які цьому перешкоджали. Розглянуто дискусійні погляди щодо проведення операції на етапі її підготовки серед представників вищого військово-політичного керівництва Німеччини.
ВІЙСЬКО ЯК ЗАПОРУКА ДЕРЖАВНОСТІ: ОСОБЛИВОСТІ МІЛІТАРНОЇ КОНЦЕПЦІЇ ОУН (б) НАПЕРЕДОДНІ НАЦИСТСЬКО-РАДЯНСЬКОГО ЗІТКНЕННЯ
Authors: Дрогобицький Ігор Іванович 
Published: April 2024

Процес національного державотворення з кінцевою метою у вигляді утвердження Української самостійної соборної держави слід вважати однією із визначальних віх діяльності Організації українських націоналістів (бандерівців). Формування повноцінного державного організму, у свою чергу, вимагало паралельної розбудови національного війська. Нагальність утворення збройних сил підкреслювалася обставинами початку Другої світової війни. Дійсність спонукала керівництво згаданої структури до пошуку можливостей та шляхів реалізації означених складових національної мети.
У матеріалах статті аналізується військовий вимір справи національного державотворення у трактуванні керівництва радикального крила українських націоналістів на тодішньому етапі. Проаналізовано концептуально-теоретичні засади процесу розбудови національних збройних сил у баченні представників проводу Організації українських націоналістів (бандерівців). Визначено територіально-адміністративні особливості вітчизняного військового будівництва.
Окреслено намагання діячів тодішнього революційного крила націоналістичного руху розгорнути справу формування збройних сил у практичному вимірі, зокрема у вигляді повстанського руху та регулярних частин «Української Національної Революційної Армії».
Представлено в узагальненій формі специфіку згаданого процесу. Загально окреслено складові концепції розбудови національних збройних сил, а також окремі етапи їхнього постання з урахуванням суспільно-політичних тенденцій розвитку окремих регіонів України.
Означений комплекс подій представлено на тлі відображення відношення окупаційних режимів до національних державницьких устремлінь. Стверджується факт того, що процес розбудови національних регулярних збройних сил та його проміжні етапи у формах Української Повстанської Армії (Волинь, 1942−1943 рр.) та Української Народної Самооборони (Галичина, 1943 р.) слід розглядати у перспективі розбудови регулярних збройних сил. Підкреслюється кінцева мета їхнього функціонування у вигляді здобуття та утвердження Української самостійної соборної держави.
A View on technology and controls for epidemic preparedness and the growth of COVID-19 disease in Saudi Arabia
Authors: Mohammad Abeer Mahmoud , Almeneem Halah Ahmad Abd , Asiri Wafa Mohammed , Mohamed Ishraga Abdullah Sulieman 
Published: April 2024

This work emphasizes that the best chance of ending the pandemic lies in the Measures that can help us respond to these epidemic threats more effectively than before in the scope of protection from epidemic risks that we were not aware of before the outbreak of the disease, which is to benefit from the study of previous experiences. The work show the importance of technology about how to prepare for and handle any pandemic by discussing a successful case from the past. We examine Saudi Arabia's COVID-19 pandemic response experiences. We proposed a case evolution study from the southern region of Jazan to demonstrate the consequences of these techniques. Data on the daily Jazan COVID-19 infection curve collected from July 1st to September 30th, 2020, was processed using multiple Python software modules, such as encoder-decoder LSTM, 1D-CNN, and Complex network analysis of time series to identify changes within the infection curve's structure. A Gaussian modeling study was performed to compare the data movement with the protection from epidemic risks computed by stratification based on the data group.The network analysis shows that hubs are stable while medium and low-degree nodes are unstable. Also, from the perspective of Gaussian modeling, the COVID-19 infection curve parts indicate that the pandemic in Saudi Arabia is about to peak and move into the second (declining) half of the bell-shaped distribution.Technology in many areas and internet access make Saudi Arabia's social separation strategies a pandemic-eradication success story
In vitro co-culture of Clostridium scindens with primary human colonic epithelium protects the epithelium against Staphylococcus aureus
Authors: Wang Hao , Kim Raehyun , Wang Yuli , Furtado Kathleen L. , Sims Christopher E. , Tamayo Rita , Allbritton Nancy L. 
Published: April 2024

A complex and dynamic network of interactions exists between human gastrointestinal epithelium and intestinal microbiota. Therefore, comprehending intestinal microbe-epithelial cell interactions is critical for the understanding and treatment of intestinal diseases. Primary human colonic epithelial cells derived from a healthy human donor were co-cultured with Clostridium scindens (C. scindens), a probiotic obligate anaerobe; Staphylococcus aureus (S. aureus), a facultative anaerobe and intestinal pathogen; or both bacterial species in tandem. The co-culture hanging basket platform used for these experiments possessed walls of controlled oxygen (O2) permeability to support the formation of an O2 gradient across the intestinal epithelium using cellular O2 consumption, resulting in an anaerobic luminal and aerobic basal compartment. Both the colonic epithelial cells and C. scindens remained viable over 48 h during co-culture. In contrast, co-culture with S. aureus elicited significant damage to colonic epithelial cells within 24 h. To explore the influence of the intestinal pathogen on the epithelium in the presence of the probiotic bacteria, colonic epithelial cells were inoculated sequentially with the two bacterial species. Under these conditions, C. scindens was capable of repressing the production of S. aureus enterotoxin. Surprisingly, although C. scindens converted cholic acid to secondary bile acids in the luminal medium, the growth of S. aureus was not significantly inhibited. Nevertheless, this combination of probiotic and pathogenic bacteria was found to benefit the survival of the colonic epithelial cells compared with co-culture of the epithelial cells with S. aureus alone. This platform thus provides an easy-to-use and low-cost tool to study the interaction between intestinal bacteria and colonic cells in vitro to better understand the interplay of intestinal microbiota with human colonic epithelium.
Exploration of agr types, virulence−associated genes, and biofilm formation ability in Staphylococcus aureus isolates from hemodialysis patients with vascular access infections
Authors: Lai Chi-Hsiang , Wong Min Yi , Huang Tsung-Yu , Kao Chih-Chen , Lin Yu-Hui , Lu Chu-Hsueh , Huang Yao-Kuang 
Published: April 2024

IntroductionStaphylococcus aureus, is a pathogen commonly encountered in both community and hospital settings. Patients receiving hemodialysis treatment face an elevated risk of vascular access infections (VAIs) particularly Staphylococcus aureus, infection. This heightened risk is attributed to the characteristics of Staphylococcus aureus, , enabling it to adhere to suitable surfaces and form biofilms, thereby rendering it resistant to external interventions and complicating treatment efforts.MethodsTherefore this study utilized PCR and microtiter dish biofilm formation assay to determine the difference in the virulence genes and biofilm formation among in our study collected of 103 Staphylococcus aureus, isolates from hemodialysis patients utilizing arteriovenous grafts (AVGs), tunneled cuffed catheters (TCCs), and arteriovenous fistulas (AVFs) during November 2013 to December 2021.ResultsOur findings revealed that both MRSA and MSSA isolates exhibited strong biofilm production capabilities. Additionally, we confirmed the presence of agr types and virulence genes through PCR analysis. The majority of the collected isolates were identified as agr type I. However, agr type II isolates displayed a higher average number of virulence genes, with MRSA isolates exhibiting a variety of virulence genes. Notably, combinations of biofilm-associated genes, such as eno−clfA−clfB−fib−icaA−icaD and eno−clfA−clfB−fib−fnbB−icaA−icaD, were prevalent among Staphylococcus aureus, isolates obtained from vascular access infections.DiscussionThese insights contribute to a better understanding of the molecular characteristics associated with Staphylococcus aureus, infections in hemodialysis patients and provided more targeted and effective treatment approaches.
Global trends and spatial drivers of diabetes mellitus mortality, 1990-2019: a systematic geographical analysis
Authors: Xu Zejia , Feng Jianheng , Xing Siyi , Liu Yin , Chen Yuting , Li Jie , Feng Yunhui 
Published: April 2024

ObjectiveDiabetes mellitus is the leading cause of death worldwide, and multiple risk factors associated with diabetes mortality.MethodsEmploying spatial statistics, we characterized the spatial distribution and patterns of diabetes mortality, and revealed the spatial relationship between diabetes mortality and 11 socioeconomic and environmental risk factors at the country level, from 1990 to 2019.ResultsGlobally, significantly high rates of diabetes mortality were primarily clustered in countries with limited land areas or located on islands, such as Fiji, Kiribati, Eswatini, and Trinidad and Tobago. Countries with weaker economic independence are more likely to have higher diabetes mortality rates. In addition, the impact of socioeconomic and environmental factors was significant at the country level, involving health expenditure, number of physicians, household and ambient air pollution, smoking, and alcohol consumption. Notably, the spatial relationship between diabetes mortality and ambient air pollution, as well as alcohol consumption, showed negative correlations. Countries with high diabetes mortality rates generally had lower levels of ambient air pollution and alcohol consumption.ConclusionThe study highlights the spatial clustering of diabetes mortality and its substantial variation. While many risk factors can influence diabetes mortality, it’s also essential to consider the level of these factors at the country level. Tailoring appropriate interventions based on specific national circumstances holds the potential to more effectively mitigate the burden of diabetes mortality.
Pegunigalsidase alfa: a novel, pegylated recombinant alpha-galactosidase enzyme for the treatment of Fabry disease
Authors: Germain Dominique P. , Linhart Ales 
Published: April 2024

Fabry disease, a rare X-linked genetic disorder, results from pathogenic variants in GLA, leading to deficient lysosomal α-galactosidase A enzyme activity and multi-organ manifestations. Since 2001, enzyme replacement therapy (ERT), using agalsidase alfa or agalsidase beta, has been the mainstay treatment, albeit with limitations such as rapid clearance and immunogenicity. Pegunigalsidase alfa, a novel PEGylated recombinant alpha-galactosidase, offers promise as an alternative. Produced in plant cells, pegunigalsidase alfa exhibits enhanced stability, prolonged half-life, and reduced immunogenicity due to pegylation. A phase 1/2 clinical trial demonstrated Gb3 clearance from renal capillary endothelial cells and its 48-month extension study revealed notable outcomes in renal function preservation. Three phase 3 clinical trials (BRIDGE, BRIGHT, and BALANCE) have shown favorable efficacy and safety profile, although caution is warranted in interpreting the results of BRIDGE and BRIGHT which lacked control groups. In BALANCE, the pivotal phase 3 trial comparing pegunigalsidase alfa with agalsidase beta, an intention-to-treat analysis of the eGFR decline over 2 years showed that the intergroup difference [95%confidence interval] in the median slope was −0.36 mL/min/1.73 m2/year [−2.44; 1.73]. The confidence interval had a lower limit above the prespecified value of −3 mL/min/1.73 m2/year and included zero. Despite challenges such as occasional hypersensitivity reactions and immune-complex-mediated glomerulonephritis, pegunigalsidase alfa approval by the European Medicines Agency and the Food and Drug Administration represents a significant addition to Fabry disease therapeutic landscape providing an option for patients in whom enzyme replacement therapy with current formulations is poorly tolerated or poorly effective.
A metformin add-on clinical study in multiple sclerosis to evaluate brain remyelination and neurodegeneration (MACSiMiSE-BRAIN): study protocol for a multi-center randomized placebo controlled clinical trial
Authors: De Keersmaecker Anna-Victoria , Van Doninck Eline , Popescu Veronica , Willem Lander , Cambron Melissa , Laureys Guy , D’ Haeseleer Miguel , Bjerke Maria , Roelant Ella , Lemmerling Marc , D’hooghe Marie Beatrice , Derdelinckx Judith , Reynders Tatjana , Willekens Barbara 
Published: April 2024

IntroductionDespite advances in immunomodulatory treatments of multiple sclerosis (MS), patients with non-active progressive multiple sclerosis (PMS) continue to face a significant unmet need. Demyelination, smoldering inflammation and neurodegeneration are important drivers of disability progression that are insufficiently targeted by current treatment approaches. Promising preclinical data support repurposing of metformin for treatment of PMS. The objective of this clinical trial is to evaluate whether metformin, as add-on treatment, is superior to placebo in delaying disease progression in patients with non-active PMS.Methods and analysisMACSiMiSE-BRAIN is a multi-center two-arm, 1:1 randomized, triple-blind, placebo-controlled clinical trial, conducted at five sites in Belgium. Enrollment of 120 patients with non-active PMS is planned. Each participant will undergo a screening visit with assessment of baseline magnetic resonance imaging (MRI), clinical tests, questionnaires, and a safety laboratory assessment. Following randomization, participants will be assigned to either the treatment (metformin) or placebo group. Subsequently, they will undergo a 96-week follow-up period. The primary outcome is change in walking speed, as measured by the Timed 25-Foot Walk Test, from baseline to 96 weeks. Secondary outcome measures include change in neurological disability (Expanded Disability Status Score), information processing speed (Symbol Digit Modalities Test) and hand function (9-Hole Peg test). Annual brain MRI will be performed to assess evolution in brain volumetry and diffusion metrics. As patients may not progress in all domains, a composite outcome, the Overall Disability Response Score will be additionally evaluated as an exploratory outcome. Other exploratory outcomes will consist of paramagnetic rim lesions, the 2-minute walking test and health economic analyses as well as both patient- and caregiver-reported outcomes like the EQ-5D-5L, the Multiple Sclerosis Impact Scale and the Caregiver Strain Index.Ethics and disseminationClinical trial authorization from regulatory agencies [Ethical Committee and Federal Agency for Medicines and Health Products (FAMHP)] was obtained after submission to the centralized European Clinical Trial Information System. The results of this clinical trial will be disseminated at scientific conferences, in peer-reviewed publications, to patient associations and the general public.Trial registrationClinicalTrials.gov Identifier: NCT05893225, EUCT number: 2023-503190-38-00.
Case report: Rubella virus-associated cutaneous granuloma in an adult with TAP1 deficiency
Authors: Wang Qiaohui , Su Huilin , Han Jiande , Yang Juhua , Lin Naiyu 
Published: April 2024

Rubella virus-associated granulomas commonly occur in immunocompromised individuals, exhibiting a diverse range of clinical presentations. These manifestations can vary from predominantly superficial cutaneous plaques or nonulcerative nodules to more severe deep ulcerative lesions, often accompanied by extensive necrosis and significant tissue destruction. TAP1 deficiency, an exceedingly rare primary immune-deficiency disorder, presents with severe chronic sino-pulmonary infection and cutaneous granulomas. This report highlights the occurrence of rubella virus-associated cutaneous granulomas in patients with TAP1 deficiency. Notably, the pathogenic mutation responsible for TAP1 deficiency stems from a novel genetic alteration that has not been previously reported. This novel observation holds potential significance for the field of diagnosis and investigative efforts in the context of immunodeficiency disorders.
Analysis of the expression level and predictive value of CLEC16A|miR-654-5p|RARA regulatory axis in the peripheral blood of patients with ischemic stroke based on biosignature analysis
Authors: Hao Jiang-jie , Liu Yuan , Lu Jun-hua , Zhao Ying , Lin Ying , Ma Li-qiu , Xue Ping , Jin Bao-yun , Li Bei-bei , Zhou Zheng , Huang Xin-xin , Liu Ting , Li Meng-yue , Lai Jin-ying , Guan Hong-jun 
Published: April 2024

IntroductionIschemic stroke (IS) is a cerebrovascular disease that can be disabling and fatal, and there are limitations in the clinical treatment and prognosis of IS. It has been reported that changes in the expression profile of circRNAs have been found during injury in ischemic stroke, and circRNAs play an important role in the IS cascade response. However, the specific mechanisms involved in the pathogenesis of IS are not yet fully understood, and thus in-depth studies are needed.MethodsIn this study, one circRNA dataset (GSE161913), one miRNA dataset (GSE60319) and one mRNA dataset (GSE180470) were retrieved from the Gene Expression Omnibus (GEO) database and included, and the datasets were differentially expressed analyzed by GEO2R and easyGEO to get the DEcircRNA, DEmiRNA and DEmRNA, and DEmRNA was enriched using ImageGP, binding sites were predicted in the ENCORI database, respectively, and the competitive endogenous RNA (ceRNA) regulatory network was visualized by the cytoscape software, and then selected by MCC scoring in the cytoHubba plugin Hub genes. In addition, this study conducted a case–control study in which blood samples were collected from stroke patients and healthy medical examiners to validate the core network of ceRNAs constructed by biosignature analysis by real-time fluorescence quantitative qRT-PCR experiments.ResultsA total of 233 DEcircRNAs, 132 DEmiRNAs and 72 DEmRNAs were screened by bioinformatics analysis. circRNA-mediated ceRNA regulatory network was constructed, including 148 circRNAs, 43 miRNAs and 44 mRNAs. Finally, CLEC16A|miR-654-5p|RARA competitive endogenous regulatory axis was selected for validation by qRT-PCR, and the validation results were consistent with the bioinformatics analysis.DiscussionIn conclusion, the present study establishes a new axis of regulation associated with IS, providing new insights into the pathogenesis of IS.
Post-stroke diabetes management: a qualitative study
Authors: Hewitt Jonathan , Azhari Hala F. , O’Neill Martin , Smith Alexander , Quinn Terence , Dawson Jesse 
Published: April 2024

IntroductionDiabetes is associated with an increased risk of stroke. In many cases, a diabetes diagnosis may predate a stroke; however, diabetes is often diagnosed during the hospital admission following a stroke. To explore the experiences of stroke survivors as they cope with a new diabetes diagnosis, particularly regarding developing an effective strategy for managing the disease.MethodsA qualitative grounded theory approach was used that employed focus group interviews with participants, including clinicians and stroke survivors, to develop a holistic understanding of primary and secondary stroke care services and the experiences of those accessing them.ResultsClinicians believed they were not optimally equipped to manage diabetes as a condition. They believed more emphasis should be placed on self-management, which would be better managed by lifestyle changes than medication alone. Conversely, stroke survivors with diabetes experienced an additional burden associated with the diagnoses but relied on clinicians to manage their diabetes and believed the clinicians were failing if they were unwilling or unable to achieve this.DiscussionThe research highlights the tensions between stroke survivors and healthcare professionals. Stroke survivors relied on the healthcare teams to provide the optimal treatment when they had recently undergone a significant health event where they had experienced a stroke and received a diabetes diagnosis. However, the healthcare teams, while recognizing the importance of a holistic and comprehensive treatment package, struggled to provide it due to resource limitations. To optimize post-stroke diabetes self-management education, a strategic framework that prioritizes patient empowerment and interdisciplinary collaboration is paramount. Tailoring educational interventions to align with individual patient profiles—considering their unique health status, personal preferences, and cultural context—is essential for fostering self-efficacy. Such a strategy not only empowers patients to take an active role in managing their diabetes post-stroke but also contributes to superior health outcomes and an elevated standard of living.