bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced representative methylome profiling of cell-free DNA for breast cancer detection
Authors: Yang Qingmo , Zhu Xingqiang , Liu Yulu , He Zhi , Xu Huan , Zheng Hailing , Huang Zhiming , Wang Dan , Lin Xiaofang , Guo Ping , Chen Hongliang 
Published: February 2024

Abstract
Background
Whole-genome methylation sequencing of cfDNA is not cost-effective for tumor detection. Here, we introduce reduced representative methylome profiling (RRMP), which employs restriction enzyme for depletion of AT-rich sequence to achieve enrichment and deep sequencing of CG-rich sequences.

Methods
We first verified the ability of RRMP to enrich CG-rich sequences using tumor cell genomic DNA and analyzed differential methylation regions between tumor cells and normal whole blood cells. We then analyzed cfDNA from 29 breast cancer patients and 27 non-breast cancer individuals to detect breast cancer by building machine learning models.

Results
RRMP captured 81.9% CpG islands and 75.2% gene promoters when sequenced to 10 billion base pairs, with an enrichment efficiency being comparable to RRBS. RRMP allowed us to assess DNA methylation changes between tumor cells and whole blood cells. Applying our approach to cfDNA from 29 breast cancer patients and 27 non-breast cancer individuals, we developed machine learning models that could discriminate between breast cancer and non-breast cancer controls (AUC = 0.85), suggesting possibilities for truly non-invasive cancer detection.

Conclusions
We developed a new method to achieve reduced representative methylome profiling of cell-free DNA for tumor detection.
The mechanistic role of natural antimicrobials in preventing Staphylococcus aureus invasion of MAC-T cells using an in vitro mastitis model
Authors: Balta Igori , McCleery David , David Saida Roxana Feier , Pet Elena , Stef Ducu , Iancu Tiberiu , Pet Ioan , Stef Lavinia , Corcionivoschi Nicolae 
Published: February 2024

Abstract
Background
Starting primarily as an inflammation of the mammary gland, mastitis is frequently driven by infectious agents such as Staphylococcus aureus. Mastitis has a large economic impact globally, which includes diagnostic, treatment, and the production costs not to mention the potential milk contamination with antimicrobial residues. Currently, mastitis prevention and cure depends on intramammary infusion of antimicrobials, yet, their overuse risks engendering resistant pathogens, posing further threats to livestock.

Methods
In our study we aimed to investigate, in vitro, using bovine mammary epithelial cells (MAC-T), the efficacy of the AuraShield an antimicrobial mixture (As) in preventing S. aureus attachment, internalisation, and inflammation. The antimicrobial mixture (As) included: 5% maltodextrin, 1% sodium chloride, 42% citric acid, 18% sodium citrate, 10% silica, 12% malic acid, 9% citrus extract and 3% olive extract (w/w).

Results and discussion
Herein we show that As can significantly reduce both adherence and invasion of MAC-T cells by S. aureus, with no impact on cell viability at all concentrations tested (0.1, 0.2, 0.5, 1%) compared with untreated controls. The anti-apoptotic effect of As was achieved by significantly reducing cellular caspase 1, 3 and 8 activities in the infected MAC-T cells. All As concentrations were proven to be subinhibitory, suggesting that Ac can reduce S. aureus virulence without bacterial killing and that the effect could be dual including a host modulation effect. In this context, we show that As can reduce the expression of S. aureus clumping factor (ClfB) and block its interaction with the host Annexin A2 (AnxA2), resulting in decreased bacterial adherence in infection of MAC-T cells. Moreover, the ability of As to block AnxA2 had a significant decreasing effect on the levels of pro inflammatory cytokine released upon S. aureus interaction with MAC-T cells.

Conclusion
The results presented in this study indicate that mixtures of natural antimicrobials could potentially be considered an efficient alternative to antibiotics in treating S. aureus induced mastitis.
Efficient improvement of energy detection technique in cognitive radio networks using K-nearest neighbour (KNN) algorithm
Authors: Musuvathi Aneesh Sarjit S. , Archbald Jofin F. , Velmurugan T. , Sumathi D. , Renuga Devi S. , Preetha K. S. 
Published: February 2024

AbstractWith the birth of the IoT era, it is evident that the existing number of devices is going to rise exponentially. Any two devices will communicate with each other using the same frequency band with limited availability. Therefore, it is of vital importance that this frequency band used for communication be used efficiently to accommodate the maximum number of devices with the available radio resources. Cognitive radio (CR) technology serves this exact purpose. The stated one is an intelligent radio that is made to automatically identify the optimal wireless channel in the available wireless spectrum at a given instant. An important functionality of CR is spectrum sensing. Energy detection is a very popular algorithm used for spectrum sensing in CR technology for efficient allocation of radio resources to the devices intended to communicate with each other. Energy detection detects the presence of a primary user (PU) signal by continuously monitoring a selected frequency bandwidth. The conventional energy detection technique is known to perform poorly in lower SNR ranges. This paper works towards the improvement of the energy detection algorithm with the help of machine learning (ML). The ML model uses the general properties of the signal as training data and classifies between a PU signal and noise at very low SNR ranges (− 25 to − 10 dB). In this research, a K-nearest neighbours (KNN) model is selected for its versatility and simplicity. Upon testing the model with an out-of-sample dataset, the KNN model produced a detection accuracy of 94.5%.
Toward tuberculosis elimination by understanding epidemiologic characteristics and risk factors in Hainan Province, China
Authors: Zhou Changqiang , Li Tao , Du Jian , Yin Dapeng , Li Xiujun , Li Shixue 
Published: February 2024

Abstract
Background
The disease burden of tuberculosis (TB) was heavy in Hainan Province, China, and the information on transmission patterns was limited with few studies. This atudy aims to further explore the epidemiological characteristics and influencing factors of TB in Hainan Province, and thereby contribute valuable scientific evidences for TB elimination in Hainan Province.

Methods
The TB notification data in Hainan Province from 2013 to 2022 were collected from the Chinese National Disease Control Information System Tuberculosis Surveillance System, along with socio-economic data. The spatial–temporal and population distributions were analyzed, and spatial autocorrelation analysis was conducted to explore TB notification rate clustering. In addition, the epidemiological characteristics of the cases among in-country migrants were described, and the delay pattern in seeking medical care was investigated. Finally, a geographically and temporally weighted regression (GTWR) model was adopted to analyze the relationship between TB notification rate and socio-economic indicators. The tailored control suggestions in different regions for TB elimination was provided by understanding epidemiological characteristics and risk factors obtained by GTWR.

Results
From 2013 to 2022, 64,042 cases of TB were notified in Hainan Province. The estimated annual percentage change of TB notification rate in Hainan Province from 2013 to 2020 was − 6.88% [95% confidence interval (CI): − 5.30%, − 3.69%], with higher rates in central and southern regions. The majority of patients were males (76.33%) and farmers (67.80%). Cases among in-country migrants primarily originated from Sichuan (369 cases), Heilongjiang (267 cases), Hunan (236 cases), Guangdong (174 cases), and Guangxi (139 cases), accounting for 53%. The majority (98.83%) of TB cases were notified through passive case finding approaches, with delay in seeking care. The GTWR analysis showed that gross domestic product per capita, the number of medical institutions and health personnel per 10,000 people were main factors affecting the high TB notification rates in some regions in Hainan Province. Different regional tailored measures such as more TB specialized hospitals were proposed based on the characteristics of each region.

Conclusions
The notification rate of TB in Hainan Province has been declining overall but still remained high in central and southern regions. Particular attention should be paid to the prevalence of TB among males, farmers, and out-of-province migrant populations. The notification rate was also influenced by economic development and medical conditions, indicating the need of more TB specialized hospitals, active surveillance and other tailored prevention and control measures to promote the progress of TB elimination in Hainan Province.
Longitudinal associations between response-style strategies and abnormal eating behaviors/attitudes in adolescents: a cross-lagged panel model
Authors: Murayama Yasuo , Ito Hiroyuki , Hamada Megumi , Takayanagi Nobuya , Nakajima Takahiro , Myogan Mitsunori , Tsujii Masatsugu 
Published: February 2024

Abstract
Background
Previous studies have suggested that response-style strategies (rumination, problem-solving, and distraction) can be risk or protective factors for the development of abnormal eating behaviors/attitudes (AEB) during adolescence. However, due to limited empirical data regarding the prospective effects of these strategies on AEB, further research is needed to clarify their role in developing AEB in adolescence.

Methods
This study investigated the one-year lagged effects of response-style strategies on AEB in 24,883 fourth- to eighth-grade students in Japan between 2015 and 2019 using a cross-lagged panel model. Depressive symptoms and body mass index (BMI), which are reported to relate to AEB, were also included in the analytic model. The students self-reported their levels of response-style strategies, AEB, and depressive symptoms. We also evaluated BMI based on teachers’ reports.

Results
We found that greater rumination significantly predicted more severe AEB in the following year among students from all grades, with small to moderate effect sizes. In addition, distraction significantly predicted more severe binge eating/purging behaviors, but with very weak small effect sizes. Problem-solving did not predict any level of AEB. Furthermore, we observed significant reciprocal relationships between response-style strategies, AEB, and depressive symptoms. Positive reciprocal associations between BMI and AEB were also found except for some intervals.

Conclusions
We concluded that a decrease in rumination is critical to alleviating mental health problems, such as AEB and depressive symptoms, during adolescence. This suggests that interventions to reduce the level of rumination should be conducted in the early stages of adolescence.
Trial Registration Number: Not Applicable.
The effect of working memory training on test anxiety symptoms and attentional control in adolescents
Authors: Xu Chunling , Wei Hua 
Published: February 2024

Abstract
Objective
The percentage of adolescents with test anxiety is increasing rapidly. Working memory (WM) training has been demonstrated to reduce anxiety levels and enhance attentional control in individuals. Therefore, we investigated whether adaptive dual n-back WM training could lower test anxiety level and improve attentional control in adolescents.

Methods
Forty adolescents were allocated to either adaptive dual n-back WM training (n = 21) or non-adaptive dual 1-back WM training (n = 19) for 10 days. The Test Anxiety Scale was applied to measure individuals’ test anxiety symptoms. The Attentional Control Scale (ACS), the flanker task, and the Go/Nogo task were used to measure attentional control.

Results
Compared with the control group, the training group reported significantly relief of test anxiety symptoms; however, there were no significant differences between the two groups in pre-to-post changes in ACS scores or performance on the flanker task and Go/Nogo task.

Conclusion
In sum, adaptive dual n-back WM training effectively reduced adolescents’ level of test anxiety but did not improve their attentional control.
Designing and evaluating tasks to measure individual differences in experimental psychology: a tutorial
Authors: Brysbaert Marc 
Published: February 2024

AbstractExperimental psychology is witnessing an increase in research on individual differences, which requires the development of new tasks that can reliably assess variations among participants. To do this, cognitive researchers need statistical methods that many researchers have not learned during their training. The lack of expertise can pose challenges not only in designing good, new tasks but also in evaluating tasks developed by others. To bridge the gap, this article provides an overview of test psychology applied to performance tasks, covering fundamental concepts such as standardization, reliability, norming and validity. It provides practical guidelines for developing and evaluating experimental tasks, as well as for combining tasks to better understand individual differences. To further address common misconceptions, the article lists 11 prevailing myths. The purpose of this guide is to provide experimental psychologists with the knowledge and tools needed to conduct rigorous and insightful studies of individual differences.
Choroid plexus volume in multiple sclerosis can be estimated on structural MRI avoiding contrast injection
Authors: Visani Valentina , Pizzini Francesca B. , Natale Valerio , Tamanti Agnese , Anglani Mariagiulia , Bertoldo Alessandra , Calabrese Massimiliano , Castellaro Marco 
Published: February 2024

AbstractWe compared choroid plexus (ChP) manual segmentation on non-contrast-enhanced (non-CE) sequences and reference standard CE T1- weighted (T1w) sequences in 61 multiple sclerosis patients prospectively included. ChP was separately segmented on T1w, T2-weighted (T2w) fluid-attenuated inversion-recovery (FLAIR), and CE-T1w sequences. Inter-rater variability assessed on 10 subjects showed high reproducibility between sequences measured by intraclass correlation coefficient (T1w 0.93, FLAIR 0.93, CE-T1w 0.99). CE-T1w showed higher signal-to-noise ratio and contrast-to-noise ratio (CE-T1w 23.77 and 18.49, T1w 13.73 and 7.44, FLAIR 13.09 and 10.77, respectively). Manual segmentation of ChP resulted 3.073 ± 0.563 mL (mean ± standard deviation) on T1w, 3.787 ± 0.679 mL on FLAIR, and 2.984 ± 0.506 mL on CE-T1w images, with an error of 28.02 ± 19.02% for FLAIR and 3.52 ± 12.61% for T1w. FLAIR overestimated ChP volume compared to CE-T1w (p < 0.001). The Dice similarity coefficient of CE-T1w versus T1w and FLAIR was 0.67 ± 0.05 and 0.68 ± 0.05, respectively. Spatial error distribution per slice was calculated after nonlinear coregistration to the standard MNI152 space and showed a heterogeneous profile along the ChP especially near the fornix and the hippocampus. Quantitative analyses suggest T1w as a surrogate of CE-T1w to estimate ChP volume.Relevance statement To estimate the ChP volume, CE-T1w can be replaced by non-CE T1w sequences because the error is acceptable, while FLAIR overestimates the ChP volume. This encourages the development of automatic tools for ChP segmentation, also improving the understanding of the role of the ChP volume in multiple sclerosis, promoting longitudinal studies.Key points • CE-T1w sequences are considered the reference standard for ChP manual segmentation.• FLAIR sequences showed a higher CNR than T1w sequences but overestimated the ChP volume.• Non-CE T1w sequences can be a surrogate of CE-T1w sequences for manual segmentation of ChP.
Graphical Abstract
Transmission of areca palm velarivirus 1 (APV1) by Pseudococcus cryptus
Authors: Zhao Xue , Zhao Ruibai , Cao Xianmei , Wang Yutian , Wang Hongxing , Huang Xi 
Published: February 2024

AbstractBetel palm is one of the most economically important crops in Southeast Asia. The occurrence and expansion of yellow leaf disease (YLD) have significantly impacted betel palm plantations. Our previous research demonstrated that areca palm velarivirus 1 (APV1) was associated with YLD and transmitted by Ferrisia virgata (striped mealybug), causing YLD in betel palms. This finding provides strong etiological evidence of the role played by APV1 in YLD. Controlling YLD is a pressing issue with significant challenges. One viable approach is to prevent the virus from spreading by disrupting the transmission vectors. Therefore, it is imperative to identify potential vectors of APV1. In this study, we detected APV1 in the stylet, foregut, midgut, and hindgut of Pseudococcus cryptus (cryptic mealybug) using immunocapture RT-PCR and in situ immunofluorescence localization. P. cryptus transmitted APV1 in a non-circulative, semi-persistent manner. The retention time of APV1 in P. cryptus was notably longer than that in F. virgata. Additionally, field investigations revealed that three other insects infesting betel palms also carried APV1. In summary, APV1 may also be transmitted by other mealybug species, and the lower specificity of transmission vectors makes it challenging to control the spread of this devastating disease. This work provides timely knowledge for the prevention and management of YLD.
Postbiotics: the new horizons of microbial functional bioactive compounds in food preservation and security
Authors: Mishra Bishwambhar , Mishra Awdhesh Kumar , Mohanta Yugal Kishore , Yadavalli Rajasri , Agrawal Dinesh Chand , Reddy Himavarshini Parvath , Gorrepati Rithika , Reddy C Nagendranatha , Mandal Sanjeeb Kumar , Shamim Mohammad Zaki , Panda Jibanjyoti 
Published: February 2024

AbstractIn recent decades, consumers, manufacturers, and researchers have been more interested in functional foods, which include probiotics, prebiotics, and postbiotics. Probiotics are live microbes that, when regulated in enough quantities, provide health benefits on the host, while the prebiotics are substrates that host microorganisms selectively use. Postbiotics are metabolites and cell-wall components that are beneficial to the host and are released by living bacteria or after lysis. Postbiotic dietary supplements are more stable than probiotics and prebiotics. Many bioactivities of postbiotics are unknown or poorly understood. Hence, this study aims to present a synopsis of the regular elements and new developments of the postbiotics including health-promoting effects, production, conceptualization of terms, bioactivities, and applications in the field of food safety and preservation. Postbiotics aid in bio preservation and the reduction of biofilm development in food due to their organic acids, bacteriocins, and other antibacterial activities. The present study examines the production of postbiotic metabolites in situ in food and the effects of external and internal food components. The antimicrobial roles, removal of biofilms, and its applications in preservation and food safety have also been discussed. This paper also explored the various aspects like manipulation of postbiotic composition in the food system and its safety measures.
Graphical Abstract
Association between homocysteine and coronary artery disease—trend over time and across the regions: a systematic review and meta-analysis
Authors: Unadkat Sumit V. , Padhi Bijaya K. , Bhongir Aparna Varma , Gandhi Aravind P. , Shamim Muhammad Aaqib , Dahiya Neelam , Satapathy Prakasini , Rustagi Sarvesh , Khatib Mahalaqua Nazli , Gaidhane Abhay , Zahiruddin Quazi Syed , Sah Ranjit , Serhan Hashem Abu 
Published: February 2024

Abstract
Background
The association of homocysteine with coronary artery disease (CAD) has been explored previously with mixed findings. The present Systematic Review and Meta-Analysis (SRMA) has assessed the pooled estimate of association between homocysteine (Hcy) and CAD, and its variation over the period and geography.

Methods
Systematic literature search was done in PubMed, Scopus and Cochrane to identify the observational studies that have reported mean Hcy among cases (CAD) and control. The SRMA was registered in PROSPERO (ID-CRD42023387675).

Results
Pooled standardized mean difference (SMD) of Hcy levels between the cases and controls was 0.73 (95% CI 0.55–0.91) from 59 studies. Heterogeneity was high (I2 94%). The highest SMD was found among the Asian studies (0.85 [95% CI 0.60–1.10]), while the European studies reported the lowest SMD between the cases and controls (0.32 [95% CI 0.18–0.46]). Meta-regression revealed that the strength of association was increasing over the years (Beta = 0.0227, p = 0.048).

Conclusions
Higher homocysteine levels might have a significant association with coronary artery diseases, but the certainty of evidence was rated low, owing to the observational nature of the studies, high heterogeneity, and publication bias. Within the population groups, Asian and African populations showed a greater strength of association than their European and American counterparts, and it also increased over the years.
Impact of treating chronic hepatitis C with direct acting antivirals on health-related quality of life: a real-life Egyptian experience
Authors: Elbadry Mohamed , Badawi Mahmoud , Youssef Naglaa , Duracinsky Martin , Saleh Shereen A. , Funk Anna , Elessawy Hagar , Rumpler Eva , Sayed Khadiga , Vasiliu Anca , Madec Yoann , Fontanet Arnaud , El-Kassas Mohamed 
Published: February 2024

Abstract
Background
Chronic hepatitis C virus (HCV) infection negatively impacts health-related quality of life (HRQL). We aimed to assess patient-reported outcomes (PROs) to evaluate the impact of treating chronic HCV with directly acting antivirals (DAAs) on HRQL.

Methods
PROs were assessed prospectively using the PROQOL-HCV questionnaire before (week 0), at the end (week 12), and after DAA treatment at week 24. HRQL was measured in six different dimensions: physical health, emotional health, future uncertainty, intimate relationships, social health, and cognitive functions.

Results
A total of 500 HCV patients receiving DAAs were enrolled; of them, 399 were included in the analysis (median age 57 years, 59% females). HRQL increased significantly between baseline, end of treatment, and week 24 for all dimensions (P < 0.001), more often for physical health in females compared to males (OR = 1.69, 95% CI = 1.1–2.5), for future uncertainty among people with diabetes (1.75, 95% CI = 1.05–2.9), and for cognitive functions among obese patients (OR = 1.98; 95% CI = 1.1–3.3). Improvement in HRQL was less common for intimate relations among females (OR = 0.47; 95% CI = 0.3–0.7) and in patients with cirrhosis (OR = 0.35, 95% CI = 0.1–0.7). Improvement in HRQL was consistently higher in < 60 years compared to ≥ 60 years patients, with a significant difference in social health (P < 0.001) and future uncertainty (P < 0.049) HRQL domains.

Conclusion
HRQL improved with DAA therapy, a relation consistent across all HRQL dimensions up to 12 weeks after the end of treatment.