bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of immortalized ovarian epithelial cells with BRCA1/2 mutation
Authors: Komatsu Hiroaki , okawa Masayo , Kazuki Yasuhiro , Kazuki kanako , Hichiwa Genki , Shimoya kazuhito , Sato Shin , Taniguchi Fuminori , Oshimura Mitsu , Harada Tasuku 
Published: March 2024

Abstract
We aimed to elucidate the mechanism underlying carcinogenesis by comparing normal and BRCA1/2-mutated ovarian epithelial cells established via Sendai virus-based immortalization. Ovarian epithelial cells (normal epithelium: Ovn; with germline BRCA1 mutation: OvBRCA1; with germline BRCA2 mutation: OvBRCA2) were infected with Sendai virus vectors carrying three immortalization genes (Bmi-1, hTERT, and SV40T). The immunoreactivity of anti-epithelial cellular adhesion molecule (EpCAM) antibodies in each cell line and cells after 25 passages was confirmed using flow cytometry. Chromosomes were identified and karyotyped to detect numerical and structural abnormalities. Total RNA extracted from the cells was subjected to human transcriptome sequencing. Highly expressed genes in each cell line were confirmed using real-time polymerase chain reaction. Immortalization techniques allowed 25 or more passages of Ovn, OvBRCA1, and OvBRCA2 cells. No anti-EpCAM antibody reactions were observed in primary cultures or after long-term passages of each cell line. Structural abnormalities in the chromosomes were observed in each cell line; however, the abnormal chromosomes were successfully separated from the normal structures via cloning. Only normal cells from each cell line were cloned. MMP1, CCL2, and PAPPA were more predominantly expressed in OvBRCA1 and OvBRCA2 cells than in Ovn cells. Immortalized ovarian cells derived from patients with germline BRCA1 or BRCA2 mutations showed substantially higher MMP1 expression than normal ovarian cells. However, the findings need to be validated in the future.
Reduction of Amine Consumption in Iron Ore Flotation through Direct Reuse of Process Water on Bench Scale
Authors: Monte Marisa Bezerra de Mello , Santos Iranildes Daniel , Veneu Diego Macedo , Moura Caio Philippe Carvalho , Hertel Cristiani 
Published: March 2024

Abstract

The present work investigated the direct reuse effect of process water containing residual amine from tailings and concentrate pulps on the performance of the cationic reverse flotation of iron ore. Iron ore sample collected after the desliming operation in Conceição Industrial Unit (Minas Gerais) was used for the bench scale flotation studies. The experiments used water recirculation for five cycles and amine to achieve the optimal flotation dosage, considering the residual amine contained in process water and according to the amine dosage used in the Conceição industrial circuit. The average recovery of amine in the aqueous phase (process water) was 46%; consequently, the consumption of the new amine fed into the circuit was reduced. The experiment results showed that the direct reuse of process water and reduction in new amine addition could be performed without negatively affecting the quality of the final iron ore concentrate (68% Fe and 1.3% SiO2). According to the results obtained in this work, process water containing residual amine can be reused in the iron ore flotation without loss of concentrate quality and flotation performance. The chemical composition of the water process is essential, and the residual amine in the aqueous phase must be controlled frequently.
Inhibition of fatty acid synthase in fibroblasts alleviates pulmonary fibrosis through the regulation of β- catenin
Authors: Lian Hui , Wan Ruyan , Yang Kun , Ma Shuaichen , Wang Yaxuan , Xu Kai , Cheng Lianhui , Zhao Wenyu , Li Yajun , Wang Lan , Yu Guoying 
Published: March 2024

Abstract
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease with poor therapeutic options while the pathogenesis keeps elusive. Herein we demonstrated that the expression of FASN was increased in fibroblasts of IPF and bleomycin-induced mouse lungs, and both TGF-β1 and overexpression of β-catenin promoted the protein and mRNA expression of FASN. In MRC-5 cells, inhibition of FASN with FASN shRNA or pharmacological inhibitor C75 restrained the proliferation and the migration of the fibroblasts, and reduced the transformation of lung fibroblasts into myofibroblasts. In addition, inhibition of FASN reduced the total protein and mRNA level of β-catenin, further decreased the protein content of β-catenin in the cytoplasm and the nucleus. These effects keep the fibroblasts in a quiescent state and contribute to the treatment of IPF. Mechanistic studies showed inhibition of FASN increased negative regulators of β-catenin including glycogen synthase kinase 3β (GSK3B) and Axin1 through FASN interaction with GSK3B and Axin1. Animal studies shown that inhibition of FASN by pharmacological inhibitor C75 alleviated bleomycin-induced pulmonary fibrosis in mice. Therefore, these results suggested that inhibition of FASN in fibroblast reduced the activity of the Wnt/β-catenin signaling pathway, effectively alleviated pulmonary fibrosis. Inhibition of FASN in fibroblasts will be a potential approach for the treatment of pulmonary fibrosis.
Physiological simulation of atrial-ventricular mechanical interaction in male rats during the cardiac cycle
Authors: Balakin Alexandr , Protsenko Yuri 
Published: March 2024

Abstract

Adequate assessment of the contribution of the different phases of atrial mechanical activity to the value of ejection volume and pressure developed by the ventricle is a complex and important experimental and clinical problem. A new method and an effective algorithm for controlling the interaction of isolated rat right atrial and right ventricular strips during the cardiac cycle were developed and tested in a physiological experiment. The presented functional model is flexible and has the ability to change many parameters (temperature, pacing rate, excitation delay, pre- and afterload levels, transfer length and force scaling coefficients) to simulate different types of cardiac pathologies. For the first time, the contribution of the duration of the excitation delay of the right ventricular strips to the amount of work performed by the muscles during the cardiac cycle was evaluated. Changes in the onset of atrial systole and the delay in activation of ventricular contraction may lead to a reduction in cardiac stroke volume, which should be considered in the diagnosis and treatment of cardiovascular disease and in resynchronization therapy.
Translational Research Approach to Social Orienting Deficits in Autism: The Role of Superior Colliculus-Ventral Tegmental Pathway
Authors: Bellone Camilla , Contestabile Alessandro , Kojovic Nada , Casarotto Giulia , Delevari Farnaz , Hagmann Patric , Schaer marie 
Published: March 2024

Abstract
Autism Spectrum Disorder (ASD) is characterized by impairments in social interaction and repetitive behaviors. A key characteristic of ASD is a decreased interest in social interactions, which affects individuals' ability to engage with their social environment. This study explores the neurobiological basis of these social deficits, focusing on the pathway between the Superior Colliculus (SC) and the Ventral Tegmental Area (VTA). Adopting a translational approach, our research used Shank3 knockout mice (Shank3-/-), which parallel a clinical cohort of young children with ASD, to investigate these mechanisms. We observed consistent deficits in social orienting across species. In children with ASD, fMRI analyses revealed a significant decrease in connectivity between the SC and VTA. Additionally, using miniscopes in mice, we identified a reduction in the frequency of calcium transients in SC neurons projecting to the VTA, accompanied by changes in neuronal correlation and intrinsic cellular properties. Notably, the interneural correlation in Shank3-/- mice and the functional connectivity of the SC to VTA pathway in children with ASD correlated with the severity of social deficits. Our findings underscore the potential of the SC-VTA pathway as a biomarker for ASD and open new avenues for therapeutic interventions, highlighting the importance of early detection and targeted treatment strategies.
A new endemic species of the genus Haloxylon Bunge ex Fenzl. (Amaranthaceae s.l.) in the flora of Kazakhstan
Authors: Osmonali Bektemir B. , Vesselova Polina V. , Kudabayeva Gulmira M. , Serikbay Ussen , Daulet Abdildanov Sh. , Nikolai Friesen 
Published: March 2024

Abstract

The article discusses the results of comparative analysis of morphological, anatomical and molecular-genetic structure of species Arthrophytum longibracteatum, Arthrophytum balchaschense, Haloxylon aphyllum). Morphological-anatomical and molecular-genetic methods were used during the study. Transverse sections were viewed using a Levenhuk Zoom&Joy microscope (China), images of transverse sections were taken with a Levenhuk D740T 5.1 camera, using the LevenhukLite programme. To clarify the correctness of species identification, the collected specimens were compared with the Type specimen of Arthrophytum balchaschense stored at the Institute of Botany and Phytointroduction (AA). Other herbarium specimens of this species stored in the AA Herbarium (Almaty, Kazakhstan) and herbarium collections: MW (Moscow, Russia), LE (St. Petersburg, Russia), TASH (Tashkent, Uzbekistan) were also reviewed. With the help of QGIS programme, a map of the location points of the studied specimens was made. The conducted studies allowed to form a proof base of clear belonging of Arthrophytum balchaschense to the genus Haloxylon. This circumstance became the reason for the necessity of nomenclatural changes consisting in assignment of Arthrophytum balchaschense to the genus Haloxylon under the name Haloxylon balchaschense (Iljin) Osmonali, Veselova & Kudab. This species has a central-eastern-North Turanian distribution, being endemic to the flora of Kazakhstan.
Effect of screw access hole on fracture resistance among 3 different implant-supported crown materials after cyclic loading: An in vitro study
Authors: Liwwattanakorn Thanus , Kamonkhantikul Krid , Homsiang Woraporn , Arksornnukit Mansuang 
Published: March 2024

Abstract

Background
The presence of screw access hole on the occlusal surface might affect the strength of an implant-supported crown. This study aimed to compare the fracture resistance among 5 mol% yttria-stabilized tetragonal zirconia polycrystal (5Y-TZP), lithium disilicate, and composite resin implant-supported premolar crowns with either a cement-retained or a combined cement- and screw-retained design after cyclic loading.
Methods
Forty-eight maxillary first premolar crowns and their abutments were attached on dental implants (ø4.0 x 10 mm) embedded in epoxy resin blocks and equally divided into 6 groups (n = 8) based on crown material (5Y-TZP (Z), lithium disilicate (L), and composite resin (C)) and restoration design (a cement-retained design and a combined cement- and screw-retained design). After 500,000 cycles of cyclic loading, the fracture resistance of each specimen was measured using a universal testing machine and analyzed using the Kruskal Wallis H test to compare between crown materials within each restoration design. The Mann-Whitney U test or independent t test was used to compare the restoration designs within each crown material (α = 0.05).
Results
The fracture loads in the Z groups with a cement-retained and a combined cement-and screw-retained designs (1514±442 N and 1171±204 N) were significantly higher than those in the L group (1077±247 N and 849±116 N) and C group (1066±213 N and 648±190 N). There was no significant difference in fracture loads between the L and C groups in both restoration designs. Within the same crown material, the fracture loads of both restoration designs in the Z group were comparable. In contrast, in the L and C groups, the fracture loads in the cement-retained groups were significantly higher than those in the combined cement- and screw-retained groups.
Conclusions
Fracture resistance of an implant-supported crown is dependent on the crown material. Z demonstrated the highest fracture resistance, followed by L and C. The presence of a screw access hole did not impact the fracture resistance in Z. In contrast, for both L and C, the fracture resistance decreased with the presence of a screw access hole.
Determination of adulteration in evening primrose oil capsules by ¹H NMR analysis and chemometric techniques
Authors: Figueiredo Alisson L. , Fernandes Cleverton S. , Fernandes Ingrid L. , Santos Patricia D. S. , Assakawa Amanda C. , Basso Ernani A. , Santos Oscar O. 
Published: March 2024

Abstract
The consumption of dietary supplements has grown exponentially over the last few years, among which the oil obtained from evening primrose seeds stands out for containing a high level of polyunsaturated fatty acids, especially γ-linolenic fatty acid, which helps to reduce effects of menopause, premenstrual tension, among other benefits. This work presents a study of the lipid quality of the eight main brands of evening primrose oil capsules on the national market, evaluated using1H nuclear magnetic resonance spectroscopy together with chemometric analysis techniques (principal component analysis and multiple linear regression). Therefore, among the commercially acquired samples, only two had a lipid profile close to the reference evening primrose oil, the others had scores ranging from 18.1 to 25.5, making them unsuitable for consumption. This was possible based on the identification of markers in the1H spectra with potential for study and application in determining the lipid quality of other encapsulated vegetable oils.
Disparities in medical recommendations from AI-based chatbots across different countries/regions
Authors: Gumilar Khanisyah Erza , Indraprasta Birama Robby , Hsu Yu-Cheng , Yiou Zih-Ying , Hong Chen , Irawan Budi , Tambunan Zulkarnain , Wibowo Bagus Mukti , Nugroho Hari , Tjokroprawiro Brahmana Askandar , Dachlan Erry Gumilar , Mulawardhana Pungky , Rahestaningtyas Eccita , Pramuditya Herlangga , Putra Very Great Eka , Waluyo Setyo Teguh , Tan Nathan R. , Folarin Royhaan , Ibrahim Ibrahim Haruna , Lin Cheng-Han , Hung Tai-Yu , Lu Ting-Fang , Chen Yen-Fu , Shih Yu-Hsiang , Wang Shao-Jing , Huang Jingshan , Yates Clayton C. , Lu Chien-Hsing , Liao Li-Na , Tan Ming 
Published: March 2024

Abstract

This study explores disparities and opportunities in healthcare information provided by AI chatbots. We focused on recommendations for adjuvant therapy in endometrial cancer, analyzing responses across four regions (Indonesia, Nigeria, Taiwan, USA) and three platforms (Bard, Bing, ChatGPT-3.5). Utilizing previously published cases, we asked identical questions to chatbots from each location within a 24-hour window. Responses were double-blinded and evaluated on relevance, clarity, depth, focus, and coherence by ten endometrial cancer experts. Our analysis revealed significant variations across different countries/regions (p < 0.001). Interestingly, Bing's responses in Nigeria consistently outperformed others (p < 0.05), excelling in all evaluation criteria (p < 0.001). Bard also performed better in Nigeria compared to other regions (p < 0.05), consistently surpassing them across all categories (p < 0.001, with relevance reaching p < 0.01). Notably, Bard's overall scores were significantly higher than those of ChatGPT-3.5 and Bing in all locations (p < 0.001). These findings highlight concerning disparities and opportunities in the quality of AI-powered healthcare information based on user location and platform. This underscores the need for further research and development to ensure equitable access to reliable medical information through AI technologies.
Poly (ε‐caprolactone)/polybutylene adipate terephthalate/hydroxyapatite blend nanocomposites: Morphology-thermal degradation kinetics relationship
Authors: Bolourian Amirali , Khasraghi Samaneh Salkhi , Zarei Sara , Mahdavi Shahla , Khonakdar Hanieh , Mousavi Rasoul , Khonakdar Hossein Ali 
Published: March 2024

Abstract

Polycaprolactone/polybutylene adipate terephthalate blends (PCL/PBAT) (90/10, 75/25, and 50/50 wt/wt) containing 1, 3, and 5 phr hydroxyapatite (HA) nanoparticles were prepared using solvent casting technique. Scanning electron microscopic studies confirmed a homogeneous morphology for the blends and nanocomposites. Some agglomeration can be recognized using Energy dispersive spectroscopy (EDS) mapping in the blends containing 5 phr HA. The DSC results confirmed the presence of nanoparticles in each phase, particularly in the crystalline region, as well wetting coefficient confirmed the localization of nanoparticles at the interface. Thermal stability and degradation kinetics were analyzed using thermogravimetric analysis (TGA). Based on the TGA results, a multi-step degradation process resulted in the blends and blend nanocomposites and the PCL/PBAT blends showed better thermal stability and exhibited higher Tmax and residual mass. PCL/PBAT blends were more stable at higher temperatures compared to PCL and PBAT. Various kinetics evaluation techniques, including Friedman, Flynn-Ozawa-Wall, and Kissinger-Akahira-Sunose methods, were utilized to determine the activation energy of degradation. PCL/PBAT blends were more difficult to thermally degrade and showed the highest degradation activation energy. Incorporating HA led to lower thermal stability and, therefore, lower degradation activation energy. Incorporation of only 5 phr of HA resulted in greater thermal stability at higher temperatures (T90%).
Field study on natural phytoplankton throughout “Bizerte City” oil spill on the south-western coast of the Mediterranean Sea
Authors: Grami Boutheina , Chkili Oumayma , Garali Sondes Melliti Ben , Kousri Kaouther Mejri , Meddeb Marouan , Chouba Lassaad , Niquil Nathalie , Hlaili Asma Sakka 
Published: March 2024

Abstract

Oil spills are recurrent worldwide. Assessing the response of phytoplankton – the basis of marine food webs – at the early stages of an oil spill and throughout its evolution is crucial to improve our understanding of the impact of oil spills on the marine environment. Field data collected 1, 4, 8 and 18 days after the “Bizerte City” oil spill showed that phytoplankton responded differentially over time. In the short term (1–8 days), picophytoplankton biomass and abundance increased, possibly due to reduced grazing. In contrast, nano- and microphytoplankton biomass decreased, probably owing to inhibited growth of species sensitive to polycyclic aromatic hydrocarbons (PAHs) – the most toxic components of oil. After 18 days, the dispersal of oil and its decreasing negative effect were accompanied by outbreaks of all size fractions. Accordingly, the phytoplankton size structure shifted throughout the oil exposure level from a prevalence of microphytoplankton after a few days toward picophytoplankton dominance. Oil pollution influenced the species composition and significantly decreased diversity indexes. In the first days, nanophytoplankton was dominated by cryptophyceae (mainly Hillea fusiformis and H. marina), while microphytoplankton was mostly represented by the pennate diatoms Pseudo-nitzschia and Nitzschia, suggesting a better resistance of these genera to oil. Algal recovery after 18 days was associated with high proliferation of nano-sized Chaetoceros and micro-sized Astrionellopsis glacialis diatoms. These results improve our knowledge of the impact of oil pollution on coastal phytoplankton communities and reinforce the idea of using them as bio-indicators.
A simple method for preparing chlorophyll free phenols from olive leaves and efficiently enriching it in refined olive oil
Authors: Huang Yunfei , He Wenqing , Wang Ruifeng , Jia Yangyang , Li Lu , Xu Yawei , Lu Yuhan , Zhang Xiaoxiao , Wu Feixue , Li Chunmei 
Published: March 2024

Abstract
The refining process almost completely removes phenols from refined olive oil (ROO). Enriching ROO with olive phenols can significantly enhance its quality and health benefits. However, current enrichment methods are inefficient and overlook the negative impact of chlorophyll present in the phenol extract on the oil. In this study, we developed a straightforward two-step dissolve and resaturate process to prepare chlorophyll-free phenols from olive leaves and efficiently enrich ROO using the ultrasonic pulsed probe method. Under optimal conditions, the total phenol content in ROO increased by 424 mg/kg, including increases of 158.5 mg/kg (37.4%) for oleacein, 29.5 mg/kg (7.0%) for hydroxytyrosol, and 17.9 mg/kg (4.2%) for oleuropein aglycone. This also significantly enhanced the antioxidant activity, oxidative stability, and some flavor characteristics of ROO. Our study offers a straightforward, practical, and effective strategy for the valuable use of olive leaves and for improving the quality of ROO.
Accumulation of microtubule associated protein tau promotes hepatocellular carcinogenesis through inhibiting autophagosome-lysosome fusion
Authors: Liu Xuemin , Hao Zhiwei , He Huanhuan , Wang Xuan , Wang Wenqi , Shu Xiji , Sun Binlian , Hu Zhiyong , Hu Shaobo , Zhou Hongyan , Liu Yuchen , Wang Jianzhi , Fu Zhengqi 
Published: March 2024

Abstract

Dysregulated expression of microtubule associated protein tau (MAPT) has been reported in a variety of human cancers. However, whether and how MAPT influences hepatocellular carcinogenesis remains elusive. This study was aimed to investigate the role and the underlying mechanism of MAPT in the proliferation, invasion, migration and sorafenib sensitivity of hepatocellular carcinoma (HCC) cells. An increased level of MAPT was found in the primary tumor samples of HCC compared with the adjacent normal liver tissues, and the increase of MAPT was positively correlated with p62 evidenced by the data obtained from The Cancer Genome Atlas (TCGA), Gene Expression Profiling Interactive Analysis (GEPIA), and human samples from HCC patients. The high MAPT expression was also correlated with a poorer survival in HCC patients demonstrated by using the GEPIA survival and Kaplan-Meier analysis. Further studies showed that MAPT overexpression promoted the growth, invasion and migration and decreased sorafenib sensitivity in HepG2 and Huh7 cells; MAPT also accelerated growth of xenograft tumors with blockage of autophagosome-lysosome fusion. Finally, overexpressing MAPT inhibited AMPK, which contributed to MAPT-induced promotion of hepatocellular carcinogenesis. In conclusion, our study provides the proof-of-concept evidence validating MAPT as an attractive HCC target.