bluuebunny/crossref_metadata_2025_split huggingface dataset:
Number of pericryptal fibroblasts correlates with density of distinct mast cell phenotypes in the crypt lamina propria of human duodenum: Implications for the homeostasis of villous architecture
Authors: Crivellato Enrico , Finato Nicoletta , Isola Miriam , Pandolfi Maura , Ribatti Domenico , Beltrami Carlo Alberto 
Published: May 2006

AbstractPericryptal fibroblasts (PFs), a class of myofibroblasts, have strongly been implicated in the regulation of villous structure because of their location close to crypts and their ability to secrete cytokines affecting intestinal epithelial cell proliferation and differentiation. Recently, mast cells (MCs) have also been involved in the homeostasis of villous architecture. As myofibroblasts arise in a wide variety of settings concurrently with a local increase in the number of tissue MCs, we calculated in this study the density of both PF and distinct pericryptal MC phenotypes in the mucosa of human duodenum showing normal, defective, or atrophic villous profiles. In addition, we evaluated the statistical association between PF‐MC densities and each pattern of villous architecture. Finally, we correlated the density of PF with the density of pericryptal MC phenotypes. For this purpose, samples taken by endoscopy from 30 patients complaining of inflammatory bowel disorders were studied by immunohistochemistry. The densities of α‐smooth muscle actin‐positive PFs as well as tryptase‐, chymase‐, and c‐kit‐positive MCs were determined in the crypt lamina propria. Villous architecture was found to be significantly associated with the number of PFs and tryptase‐, chymase‐, c‐kit‐positive MCs in the lamina propria (ANOVA group effect P < 0.001). High density of both PFs and MCs was found in intestinal samples with normal villous morphology while lower densities were associated with defective or atrophic villous profiles (Tukey's test for multiple comparison P < 0.001). In addition, a significant correlation was found between PF density and the density of each pericryptal MC phenotype (vs. tryptase‐positive MCs, r = 0.913; vs. chymase‐positive MC, r = 0.905; vs. c‐kit‐positive MC, r = 0.927; P < 0.001 in all cases). This study provides morphological support for an important cooperation between PFs and MCs in maintaining normal villous architecture. Anat Rec Part A 288A:593–600, 2006. © 2006 Wiley‐Liss, Inc.
Cortical, callosal, and thalamic connections from primary somatosensory cortex in the naked mole‐rat (Heterocephalus glaber), with special emphasis on the connectivity of the incisor representation
Authors: Henry Erin C. , Catania Kenneth C. 
Published: May 2006

AbstractWe investigated the distribution of cortical, callosal, and thalamic connections from the primary somatosensory area (S1) in naked mole‐rats, concentrating on lower incisor and forelimb representations. A neuronal tracer (WGA‐HRP) was injected into the center of each respective representation under guidance from microelectrode recordings of neuronal activity. The locations of cells and terminals were determined by aligning plots of labeled cells with flattened cortical sections reacted for cytochrome oxidase. The S1 lower incisor area was found to have locally confined intrahemispheric connections and longer connections to a small cluster of cells in the presumptive secondary somatosensory (S2) and parietal ventral (PV) incisor fields. The S1 incisor area also had sparse connections with anterior cortex, in presumptive primary motor cortex. Homotopic callosal projections were identified between the S1 lower incisor areas in each hemisphere. Thalamocortical connections related to the incisor were confined to ventromedial portions of the ventral posterior medial subnucleus (VPM) and posterior medial nucleus (Po). Injections into the S1 forelimb area revealed reciprocal intrahemispheric connections to S2 and PV, to two areas in frontal cortex, and to two areas posterior to S1 that appear homologous to posterior lateral area and posterior medial area in rats. The S1 forelimb representation also had callosal projections to the contralateral S1 limb area and to contralateral S2 and PV. Thalamic distribution of label from forelimb injections included ventral portions of the ventral posterior lateral subnucleus (VPL), dorsolateral Po, the ventral lateral nucleus, and the ventral medial nucleus and neighboring intralaminar nuclei. Anat Rec Part A 288A:626–645, 2006. © 2006 Wiley‐Liss, Inc.
Solution scattering studies of conformation stability of xylanase XYNII from Trichoderma longibrachiatum
Authors: Kozak Maciej 
Published: May 2006

AbstractXylanase (endo‐1,4‐β‐xylanase; EC 3.2.1.8) is an enzyme that catalyzes the hydrolysis reaction of xylan. The structure of the xylanase II (XYNII) molecule from Trichoderma longibrachoatum (formerly Trichoderma reesei) in a solution and at different pH values has been studied by small‐ and wide‐angle scattering of synchrotron radiation (SAXS‐WAXS). Analysis of the radius of gyration that characterizes xylanase has confirmed the stability of the above enzyme structure (the radius of gyration varied from 1.65 to 1.74 nm). On the basis of the SAXS data, the low‐resolution structure of the xylanase molecule in solution has been reconstructed by using ab initio methods and programs DALAI_GA and DAMMIN. The full SAXS‐WAXS data set (0.15 > s > 9.5 nm−1) fed to the program GASBOR permitted us to construct a chain‐like spatial distribution of a dummy residues model of the xylanase molecule. The shape of the model molecules is similar to that of xylanase molecule in the crystal and shows the characteristic asymmetry that makes the molecule to resemble a right hand. © 2006 Wiley Periodicals, Inc. Biopolymers 83:95–102, 2006This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Catheterization in neonates with pulmonary atresia with intact ventricular septum
Authors: Qureshi Shakeel A. 
Published: May 2006

AbstractCardiac catheterization is infrequently required in babies with pulmonary atresia with intact ventricular septum. The main indications are to decide on the best form of treatment, the options being determined by the right ventricular as well as infundibular morphology and the presence of right‐ventricle dependent coronary circulation. In most cases, an interventional approach is appropriate. This can consist of radiofrequency or laser perforation of the atretic pulmonary valve, combined with balloon dilation. In some patients, additional stenting of the arterial duct may be needed.© 2006 Wiley‐Liss, Inc.
Late complete atriovenous block after percutaneous closure of a perimembranous ventricular septal defect
Authors: Butera Gianfranco , Massimo Chessa , Mario Carminati 
Published: May 2006

AbstractPercutaneous closure of a perimembranous ventricular septal defect (VSD) is an alternative to surgical closure. Complications include arrhythmias and in particular early postoperative complete atrioventricular (AV) block. We report the cases of two patients, aged 2.5 and 4 years, treated with an eccentric VSD occluder device whom developed complete AV block 4 and 12 months after the procedure. The first patient experienced syncope, while the second one was completely asymptomatic. Both subjects underwent endocardial ventricular pacemaker implantation. © 2006 Wiley‐Liss, Inc.
Hyperoxemic perfusion of the left anterior descending coronary artery after primary angioplasty in anterior ST‐elevation myocardial infarction
Authors: Trabattoni Daniela , Bartorelli Antonio L. , Fabbiocchi Franco , Montorsi Piero , Ravagnani Paolo , Pepi Mauro , Celeste Fabrizio , Maltagliati Anna , Marenzi Giancarlo , O'Neill William W. 
Published: May 2006

AbstractObjectives:To assess left ventricle function recovery, ST‐segment changes, and enzyme kinetic in ST‐elevation myocardial infarction patients treated with intracoronary hyperoxemic perfusion (IHP) after primary percutaneous coronary intervention and compare them with the results obtained in control patients.Background:IHP has been shown to attenuate microvascular reperfusion injury, which may result in poor LV function recovery despite successful primary percutaneous coronary intervention.Methods:Twenty seven anterior ST‐elevation myocardial infarction patients treated ≤12 hr after symptom onset by primary percutaneous coronary intervention were subjected to selective IHP into the left anterior descending coronary artery for 90 min. They were compared with 24 anterior ST‐elevation myocardial infarction control patients matched in clinical and angiographic characteristics and treated with conventional primary percutaneous coronary intervention. Left ventricular function recovery was evaluated by serial 2D contrast echocardiography.Results:Left anterior descending coronary artery recanalization was successful in all patients. After IHP (100% successful, duration 90 ± 5.4 min), patients showed a 4.8 ± 2.2 hr shorter time‐to‐peak creatine kinase release (P = 0.001), a shorter creatine kinase half‐life period (23.4 ± 8.9 hr vs. 30.5 ± 5.8 hr, P = 0.006), and a higher rate of complete ST‐segment resolution (78% vs. 42%, P = 0.01). A significant improvement of mean left ventricular ejection fraction (from (44 ± 9)% to (55 ± 11)%, P < 0.001) and wall motion score index (from 1.77 ± 0.2 to 1.39 ± 0.4, P < 0.001) was observed at 3 months in IHP patients only.Conclusion:After successful primary coronary intervention, IHP is associated with significant left ventricular function recovery when compared to conventional treatment. Enzyme kinetic and ST‐segment changes suggest faster and more complete microvascular reperfusion and may explain the salutary effects of this new therapy on left ventricular function. © 2006 Wiley‐Liss., Inc.
Influence of plaque calcium on neointimal hyperplasia following bare metal and drug‐eluting stent implantation
Authors: Shimada Yoshihisa , Kataoka Toru , Courtney Brian K. , Morino Yoshihiro , Bonneau Heidi N. , Yock Paul G. , Grube Eberhard , Honda Yasuhiro , Fitzgerald Peter J. 
Published: May 2006

AbstractObjectives:To examine the influence of vessel wall calcium on neointimal hyperplasia (NIH) following bare metal stent (BMS) and drug‐eluting stent (DES) implantation.Background:While procedural complications with coronary stenting in calcified lesions are well reported, little is known about subsequent NIH on plaque calcium following either BMS or DES implantation.Methods:In the Study to COmpare REstenosis Rate between QueST and QuaDDS‐QP2 (SCORE) trial, 6 months follow‐up volumetric intravascular ultrasound data were available for 41 lesions (BMS, 19; DES, 22). NIH thicknesses on superficial, deep, and noncalcified plaque were calculated at every 0.5 mm intervals over the stented segment. Calcified and less‐calcified cross‐sections were defined as those containing arcs of plaque calcium ≥90° and <90°, respectively.Results:In BMS, mean NIH thickness on both superficial (0.24 ± 0.23 mm) and deep calcium (0.25 ± 0.21 mm) was significantly smaller than that of noncalcified plaque (0.31 ± 0.22 mm) (P < 0.0005). NIH area was significantly smaller in calcified cross‐sections compared to less‐calcified cross‐sections (2.1 ± 1.2 mm2 vs. 3.1 ± 1.9 mm2, P < 0.0001). While in contrast, in DES, mean NIH thickness was similar, irrespective of the presence or location of calcium (0.03 ± 0.05 mm vs. 0.03 ± 0.06 mm vs. 0.03 ± 0.05 mm, superficial vs. deep vs. noncalcified plaque, P = NS). NIH area was also similar between calcified and less‐calcified cross‐sections (0.3 ± 0.6 mm2 vs. 0.3 ± 0.6 mm2, P = NS).Conclusions:These results suggest that while plaque calcium may influence NIH following BMS implantation, NIH suppression using DES does not appear to be affected by the presence or location of calcium. © 2006 Wiley‐Liss., Inc.
Successful percutaneous closure of paraprosthetic aorto‐right ventricular leak using the Amplatzer duct occluder
Authors: Dussaillant Gastón R. , Romero Leopoldo , Ramírez Alfredo , Sepúlveda Luis 
Published: May 2006

AbstractA 55‐year‐old male with persisting aorto‐right ventricular paraprosthetic leak after mitroaortic valve replacement was hospitalized for recurrent heart failure. Depressed left ventricular ejection fraction and severe pulmonary hypertension with increased right and left ventricular filling pressures were associated with significant left to right shunting through the leak. Elective closure of the leak was obtained with a 6–4 mm Amplatzer duct occluder. No complications were observed, and the patient experienced complete resolution of heart failure symptoms, with NYHA class I heart failure 12 months after discharge. © 2006 Wiley‐Liss, Inc.
Successful thrombus extraction with the Rescue™ thrombus management system during acute percutaneous coronary intervention improves flow but does not necessarily restore optimal myocardial tissue perfusion
Authors: Vijayalakshmi Kunadian , Kunadian Babu , Wright Robert A. , Sutton Andrew G.C. , Hall James A. , de Belder Mark A. 
Published: May 2006

AbstractWe determined the effectiveness of the Rescue™ device in restoring flow and achieving optimal tissue perfusion during percutaneous coronary intervention (PCI) in thrombus‐laden coronary arteries. Methods: A total of 30 patients with an acute coronary syndrome underwent PCI using the Rescue device. Results: The mean age was 65.5 ± 9.8 years. Although the Rescue device could not be passed across the lesion in 6 (20%) cases, debris was aspirated in 26 (87%). The uncorrected TIMI frame count improved following the use of the Rescue device and improved further at the completion of PCI (92.2 ± 23.9 pre‐PCI vs. 38.7 ± 31.3 post‐Rescue vs. 21.96 ± 24 post‐PCI, P < 0.0001). The thrombus score improved from 4 ± 0 to 2.2 ± 1.29 to 0.86 ± 1.4 (P < 0.0001). TIMI flow grade (TFG) 3 was restored in 60% of cases following the Rescue device and in 87% after PCI. Myocardial blush grade 3 occurred in only 13% of patients following the Rescue device and 37% of patients after PCI. Conclusion: These data suggest that the Rescue device can aspirate considerable amounts of debris in the majority of patients and significantly improves the angiographic epicardial coronary blood flow. How effective such a device is in improving tissue perfusion and, thereby, clinical outcomes for patients remains to be seen. © 2006 Wiley‐Liss Inc.
Failure of transradial approach during coronary interventions: Anatomic considerations
Authors: Valsecchi Orazio , Vassileva Angelina , Musumeci Giuseppe , Rossini Roberta , Tespili Maurizio , Guagliumi Giulio , Mihalcsik Laurian , Gavazzi Antonello , Ferrazzi Paolo 
Published: May 2006

AbstractThe anatomy of the radial artery has yet to be systematically studied from the perspective of using it as a route for catheter access. We prospectively performed angiography of the arteries of the upper limb to delineate the anatomic features of the radial artery as a way to determine the feasibility of using it as a route for coronary intervention. We studied 2,211 consecutive patients submitted to transradial cardiac catheterization. In all patients, an angiography of the upper limb arteries was performed before and after procedure. Radial puncture was successful in 98.9% of patients. At angiography, anatomic variations of upper limb arteries were noted in 505 patients (22.8%) and included tortuous configurations (3.8%), stenosis (1.7%), hypoplasias (7.7%), radioulnar loop (0.8%), abnormal origin of the radial artery (8.3%), and lusoria subclavian artery (0.45%). Overall procedural success by transradial approach was 97.5%. Patients with anatomic variations of radial artery had a significantly lower puncture (96.2% vs 99.7%, P < 0.0001) and procedural (93.1% vs 98.8%, P < 0.0001) success. The procedure was successfully performed by radial approach in 98.8% of patients with tortuous configurations, 91.9% of radial stenosis, 93.9% of hypoplastic radial artery, 83.3% of radioulnar loop, 96.7% of radial axillary origin, and 60% of lusoria subclavian artery setting. Anatomic variations of the radial artery are not rare. However, they do not represent an important limitation in transradial approach if they are well documented previously. © 2006 Wiley‐Liss., Inc.
Development of a novel catheter preventing the outflow of debris and thrombus on percutaneous transluminal angioplasty for hemodialysis access fistulas
Authors: Horita Yuki , Namura Masanobu , Matsumura Masami 
Published: May 2006

AbstractIf a large amount of debris or thrombus dislodges from a lesion because of dilation of the stenotic or occluded lesion in the hemodialysis access fistula when angioplasty is performed, it may move into the pulmonary artery via the central vein, resulting in pulmonary embolism. A novel sheath has been developed to prevent the outflow of debris or thrombus to the central vein. The catheter consists of a 5 F sheath introducer and a silicone balloon attached at the tip of the sheath. The silicon balloon is inflated via small inflation lumen on the shaft wall of the sheath. In the case of percutaneous transluminal angioplasty (PTA), the silicone balloon is inflated to block the blood flow by a mixture of contrast medium and physiological saline, and then dilate a stenotic or occluded lesion by PTA balloon. Next, the debris and thrombus are aspirated using the flush‐lumen of this device. Finally, the silicone balloon is deflated and the dilation of the lesion is confirmed. A case of hemodialysis access fistula graft with massive thrombotic occlusion was presented. This device enabled Fogarty procedure using PTA balloon and the blood flow of the graft was completely improved and a large amount of thrombus was removed. This novel device is useful to prevent the embolic complication of the intervention for hemodialysis access fistula. © 2006 Wiley‐Liss, Inc.
Bifurcation stenting with drug eluting stents: Illustration of the crush technique
Authors: Sianos Georgios , Vaina Sofia , Hoye Angela , Serruys Patrick W. 
Published: May 2006

AbstractA number of percutaneous stenting techniques have been proposed for optimal treatment of bifurcation lesions. However, to date the most favorable interventional approach still remains controversial, since bifurcation lesions are associated with high procedural complication and restenosis rates. In the era of drug‐eluting stents, crush stenting technique simplified the procedure, concurrently allowing full lesion coverage. The purpose of the present report is to review three cases treated with crush stenting and to describe in detail the technique and its variations. © 2006 Wiley‐Liss., Inc.
Perforation complicating carotid artery stenting: The use of a covered stent
Authors: Dieter Robert S. , Ikram Sohail , Satler Lowell F. , Babrowicz Joseph C. , Reddy Bhagat , Laird John R. 
Published: May 2006

AbstractCarotid artery angioplasty and stenting has emerged as an effective strategy for the treatment of significant carotid artery stenosis. Perforation during carotid artery stenting is a very rare potential complication of the procedure. We describe a case of carotid artery perforation that occurred during postdilation of the carotid artery stent. In our case this potentially serious complication was successfully treated with a covered stent‐graft. © 2006 Wiley‐Liss, Inc.
Successful utilization of a novel aspiration thrombectomy catheter (Pronto) for the treatment of patients with stent thrombosis
Authors: Siddiqui Danish S. , Choi C. Joon , Tsimikas Sotirios , Mahmud Ehtisham 
Published: May 2006

AbstractStent thrombosis is a rare but catastrophic complication of percutaneous coronary intervention (PCI). Thrombolytic therapy is ineffective for the treatment of patients with stent thrombosis, while primary PCI in such patients is limited by a high thrombus burden in the culprit vessel often leading to distal embolization and extensive tissue infarction. We present three patients with stent thrombosis successfully treated with the adjunctive use of a novel and new aspiration thrombectomy device (Pronto). © 2006 Wiley‐Liss., Inc.
Extreme coronary guide catheter support: Report of two cases of a novel telescopic guide catheter system
Authors: Stys Adam T. , Lawson William , Brown David 
Published: May 2006

AbstractExtra support of the guiding catheter is required in selected cases of percutaneous coronary intervention (PCI). We describe two successful cases of PCI of very calcified and tortuous right coronary arteries in which a novel telescopic guide system was devised. The system utilizes a long sheath that “armors” the guide catheter. The operator can adjust the support of the guiding system from soft up to extremely stiff. © 2006 Wiley‐Liss, Inc.
Use of a branch wire to anchor stents for exact placement proximal to bifurcation stents: The reverse Szabo technique
Authors: Lo Henry , Kern Morton J. 
Published: May 2006

AbstractThis report describes a technique for correct positioning of a stent proximal to a bifurcation lesion treated with V‐stents. A second wire passed through the first cell of a stent, the opposite end of the anchor wire technique as first described by Szabo et al, facilitated precise stent placement and eliminated erroneous positioning inside or outside the treated bifurcation lesion. © 2006 Wiley‐Liss, Inc.
Transgenic mice expressing tamoxifen‐inducible Cre for somatic gene modification in renal epithelial cells
Authors: Lantinga‐van Leeuwen Irma S. , Leonhard Wouter N. , van de Wal Annemieke , Breuning Martijn H. , Verbeek Sjef , de Heer Emile , Peters Dorien J.M. 
Published: May 2006

AbstractGene inactivation often leads to an embryonic‐lethal phenotype. In focal diseases like renal cell carcinomas and polycystic kidney disease, somatic gene inactivation in subsets of cells is likely to occur at later stages. We generated a transgenic mouse line with an inducible form of Cre recombinase for conditional gene modifications in kidney epithelial cells. To this end a 1.4‐kb promoter fragment of the kidney‐specific cadherin gene (KspCad) was cloned upstream of a tamoxifen‐inducible Cre recombinase (CreERT2) encoding sequence. Expression and activity of Cre was evaluated using reverse transcriptase polymerase chain reaction (RT‐PCR) analysis and by crossbreeding to Z/EG reporter mice. One KspCad‐CreERT2 line showed kidney‐specific Cre expression and mediated recombination upon tamoxifen treatment in Z/EG reporter mice. No reporter gene expression was detected in untreated animals or in extrarenal tissues upon treatment. Within the kidneys, enhanced green fluorescent protein (EGFP) fluorescence was observed in epithelial cells in several nephronic segments. In addition, the system successfully recombined a floxed Pkd1 gene. genesis 44:225–232, 2006. © 2006 Wiley‐Liss, Inc.
Temporal regulation of Cre recombinase activity in neural stem cells
Authors: Imayoshi Itaru , Ohtsuka Toshiyuki , Metzger Daniel , Chambon Pierre , Kageyama Ryoichiro 
Published: May 2006

AbstractNeural stem cells are known to give rise to distinct subtypes of neurons and glial cells over time by changing their competency. However, precise characterization of neural stem cells at various developmental stages remains to be performed. For such analysis, a tool to manipulate neural stem cells at different time points is necessary. Here, we generated transgenic mice that express Cre‐ERT2 in the ventricular zone of the developing nervous system under the control of the nestin promoter and enhancer (Nes‐CreERT2). In mice expressing Cre‐ERT2 at appropriate levels, Cre recombinase activity was mostly inactive but efficiently activated by tamoxifen within 1 day. When such mice were crossed with the ROSA‐26 or Z/EG reporter mice, neural stem cells were permanently labeled after administration of tamoxifen. Thus, Nes‐CreERT2 mice offer a powerful tool to manipulate neural stem cells genetically at desired time points. genesis 44:233–238, 2006. © 2006 Wiley‐Liss, Inc.