bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penta-1,2,3,4,6-O-galloyl-β-d-glucose induces p53 and inhibits STAT3 in prostate cancer cellsin vitroand suppresses prostate xenograft tumor growthin vivo
Authors: Hu Hongbo , Lee Hyo-Jeong , Jiang Cheng , Zhang Jinhui , Wang Lei , Zhao Yan , Xiang Qiu , Lee Eun-Ok , Kim Sung-Hoon , Lü Junxuan 
Published: September 2008

AbstractPenta-1,2,3,4,6-O-galloyl-β-d-glucose (PGG) is a naturally occurring gallotannin from some Oriental herbs. Several cell culture studies suggested a potential for PGG as a novel agent for the chemoprevention and treatment of cancer. Here, we investigated the cell death signaling mechanisms induced by PGG in human prostate cancer cells of different p53 functional status. We observed the induction of G1- and S-phase arrests and caspase-mediated apoptosis in the androgen-dependent human LNCaP cells, which express wild-type p53, and in the androgen-independent, p53-mutant DU145 cells. In LNCaP cells, caspase-mediated apoptosis induction by PGG was associated with and mediated in major part by activation of p53 as established through small interfering RNA knockdown and dominant-negative mutant approaches. Intracellular reactive oxygen species production by PGG was found to be crucial for these molecular and cellular actions. In DU145 cells, which harbor constitutively active signal transducer and activator of transcription 3 (STAT3), caspase-mediated apoptosis induction by PGG was associated with an inhibition of STAT3 Tyr705 phosphorylation and the down-regulation of STAT3 transcriptional targets Bcl-XL and Mcl-1. Overexpression of Bcl-XL or knockdown of its binding partner Bak attenuated apoptosis induction. Furthermore, we provide, for the first time, in vivo data that PGG significantly inhibited DU145 xenograft growth in an athymic nude mouse model in association with an inhibition of pSTAT3. Our data support PGG as a multitargeting agent for chemoprevention and therapy of prostate cancer by activating the p53 tumor suppressor pathway and by inhibiting STAT3 oncogenic signaling. [Mol Cancer Ther 2008;7(9):2681–91]
N-(4-Hydroxyphenyl)retinamide increases dihydroceramide and synergizes with dimethylsphingosine to enhance cancer cell killing
Authors: Wang Hongtao , Maurer Barry J. , Liu Yong-Yu , Wang Elaine , Allegood Jeremy C. , Kelly Samuel , Symolon Holly , Liu Ying , Merrill, Alfred H. , Gouazé-Andersson Valérie , Yu Jing Yuan , Giuliano Armando E. , Cabot Myles C. 
Published: September 2008

Abstract
Fenretinide [N-(4-hydroxyphenyl)retinamide (4-HPR)] is cytotoxic in many cancer cell types. Studies have shown that elevation of ceramide species plays a role in 4-HPR cytotoxicity. To determine 4-HPR activity in a multidrug-resistant cancer cell line as well as to study ceramide metabolism, MCF-7/AdrR cells (redesignated NCI/ADR-RES) were treated with 4-HPR and sphingolipids were analyzed. TLC analysis of cells radiolabeled with [3H]palmitic acid showed that 4-HPR elicited a dose-responsive increase in radioactivity migrating in the ceramide region of the chromatogram and a decrease in cell viability. Results from liquid chromatography/electrospray tandem mass spectrometry revealed large elevations in dihydroceramides (N-acylsphinganines), but not desaturated ceramides, and large increases in complex dihydrosphingolipids (dihydrosphingomyelins, monohexosyldihydroceramides), sphinganine, and sphinganine 1-phosphate. To test the hypothesis that elevation of sphinganine participates in the cytotoxicity of 4-HPR, cells were treated with the sphingosine kinase inhibitor d-erythro-N,N-dimethylsphingosine (DMS), with and without 4-HPR. After 24 h, the 4-HPR/DMS combination caused a 9-fold increase in sphinganine that was sustained through +48 hours, decreased sphinganine 1-phosphate, and increased cytotoxicity. Increased dihydrosphingolipids and sphinganine were also found in HL-60 leukemia cells and HT-29 colon cancer cells treated with 4-HPR. The 4-HPR/DMS combination elicited increased apoptosis in all three cell lines. We propose that a mechanism of 4-HPR–induced cytotoxicity involves increases in dihydrosphingolipids, and that the synergy between 4-HPR and DMS is associated with large increases in cellular sphinganine. These studies suggest that enhanced clinical efficacy of 4-HPR may be realized through regimens containing agents that modulate sphingoid base metabolism. [Mol Cancer Ther 2008;7(9):2967–76]
Granzyme B-H22(scFv), a human immunotoxin targeting CD64 in acute myeloid leukemia of monocytic subtypes
Authors: Stahnke Bettina , Thepen Theo , Stöcker Michael , Rosinke Reinhard , Jost Edgar , Fischer Rainer , Tur Mehmet K. , Barth Stefan 
Published: September 2008

AbstractAcute myeloid leukemia (AML) cells of subtypes M4 and M5 show enhanced expression of CD64 (FcγRI), the high-affinity receptor for IgG, which is normally expressed at high levels only on activated cells of the myeloid lineage. CD64 is therefore a prime target for the specific delivery of cytotoxic agents. A promising toxin candidate is granzyme B, a human serine protease originating from cytotoxic granules of CD8+ T lymphocytes and natural killer cells. After evaluating the sensitivity of the AML-related cell line U937 toward cytosolic granzyme B, we genetically fused granzyme B to H22, a humanized single-chain antibody fragment (scFv) specific for CD64, to obtain Gb-H22(scFv), a fusion protein lacking the immunogenic properties of nonhuman immunofusions. Gb-H22(scFv) was successfully expressed in human 293T cells, secreted, and purified from cell culture supernatants. The purified protein bound specifically to CD64+ U937 cells. Despite linkage to the binding domain, the proteolytic activity of functional Gb-H22(scFv) was identical to that of free granzyme B. Target cell-specific cytotoxicity was observed with a half-maximal inhibitory concentration (IC50) between 1.7 and 17 nmol/L. In addition, the induction of apoptosis in U937 cells was confirmed by Annexin A5 staining and the detection of activated caspase-3 in the cytosol. Finally, apoptosis was observed in primary CD64+ AML cells, whereas CD64− AML cells were unaffected. This is the first report of a completely human granzyme B-based immunotoxin directed against CD64, with activity against an AML-related cell line and primary AML cells. [Mol Cancer Ther 2008;7(9):2924–32]
Marks of Oppression: A Postcolonial Reading of Paul's Stigmata in Galatians 6:7
Authors: Barrier Jeremy 
Published: September 2008

AbstractIn this essay I examine Gal. 6.17 in hopes of recovering an interpretation of Paul that demonstrates Paul's self-identity as a slave. To be more specific, I investigate Gal. 6.11-17 in light of current studies in postcolonialism, in order to see the influences that the Roman Empire had upon Paul in regard to Paul's understanding of his stigmata ('marks'). The purpose of this article is (1) to evaluate the contemporary historical and social-scientific interpretations of the stigmata and argue for the importance of understanding these 'marks' in light of ancient rhetoric, (2) I will argue to situate this discussion within a postcolonial dialogue with a specific definition of postcolonialism that rejects overly simplistic 'dualistic' rubrics and investigates a text looking for domination/coordination/subordination relationships, (3) reconsider Paul's stigmata in light of the slavery metaphor by comparing Paul's stigmata to the ancient slave concept of basanos, and (4) offer a new interpretation of the stigmata as it reveals Paul's suppressed status as a colonized Jew looking for an alternative language to express his deep need for a master worthy of his loyalty. The results may be less than desirable, but I hope to both make people aware of the slave metaphor, and stimulate thought as to other meaningful metaphors that might be considered to understand Paul's relationship to Christ as it is conceived within a postcolonial optic.
Storm-Relative Helicity Revealed from Polarimetric Radar Measurements
Authors: Kumjian Matthew R. , Ryzhkov Alexander V. 
Published: September 2008

Abstract
The dual-polarization radar variables are especially sensitive to the microphysical processes of melting and size sorting of precipitation particles. In deep convective storms, polarimetric measurements of such processes can provide information about the airflow in and around the storm that may be used to elucidate storm behavior and evolution. Size sorting mechanisms include differential sedimentation, vertical transport, strong rotation, and wind shear. In particular, winds that veer with increasing height typical of supercell environments cause size sorting that is manifested as an enhancement of differential reflectivity (ZDR) along the right or inflow edge of the forward-flank downdraft precipitation echo, which has been called the ZDR arc signature. In some cases, this shear profile can be augmented by the storm inflow. It is argued that the magnitude of this enhancement is related to the low-level storm-relative environmental helicity (SRH) in the storm inflow.
To test this hypothesis, a simple numerical model is constructed that calculates trajectories for raindrops based on their individual sizes, which allows size sorting to occur. The modeling results indicate a strong positive correlation between the maximum ZDR in the arc signature and the low-level SRH, regardless of the initial drop size distribution aloft. Additional observational evidence in support of the conceptual model is presented. Potential changes in the ZDR arc signature as the supercell evolves and the low-level mesocyclone occludes are described.
How Well Do Atmospheric General Circulation Models Capture the Leading Modes of the Interannual Variability of the Asian–Australian Monsoon?
Authors: Zhou Tianjun , Wu Bo , Wang Bin 
Published: September 2008

Abstract
The authors evaluate the performances of 11 AGCMs that participated in the Atmospheric Model Intercomparison Project II (AMIP II) and that were run in an AGCM-alone way forced by historical sea surface temperature covering the period 1979–99 and their multimodel ensemble (MME) simulation of the interannual variability of the Asian–Australian monsoon (AAM). The authors explore to what extent these models can reproduce two observed major modes of AAM rainfall for the period 1979–99, which account for about 38% of the total interannual variances. It is shown that the MME SST-forced simulation of the seasonal rainfall anomalies reproduces the first two leading modes of variability with a skill that is comparable to the NCEP/Department of Energy Global Reanalysis 2 (NCEP-2) in terms of the spatial patterns and the corresponding temporal variations as well as their relationships with ENSO evolution. Both the biennial tendency and low-frequency components of the two leading modes are captured reasonably in MME. The skill of AMIP simulation is seasonally dependent. December–February (DJF) [July–August (JJA)] has the highest (lowest) skill. Over the extratropical western North Pacific and South China Sea, where ocean–atmosphere coupling may be critical for modeling the monsoon rainfall, the MME fails to demonstrate any skill in JJA, while the reanalysis has higher skills. The MME has deficiencies in simulating the seasonal phase of two anticyclones associated with the first mode, which are not in phase with ENSO forcing in observations but strictly match that of Niño-3.4 SST in MME. While the success of MME in capturing essential features of the first mode suggests the dominance of remote El Niño forcing in producing the predictable portion of AAM rainfall variability, the deficiency in capturing the seasonal phase implies the importance of local air–sea coupling effects. The first mode generally concurs with the turnabout of El Niño; meanwhile, the second mode is driven by La Niña at decaying stage. Multimodel intercomparison shows that there are good relationships between the simulated climatology and anomaly in terms of the degree of accuracy.
An Intercomparison of ERS-Scat and AMSR-E Soil Moisture Observations with Model Simulations over France
Authors: Rüdiger Christoph , Calvet Jean-Christophe , Gruhier Claire , Holmes Thomas R. H. , de Jeu Richard A. M. , Wagner Wolfgang 
Published: September 2008

Abstract
This paper presents a study undertaken in preparation of the work leading up to the assimilation of Soil Moisture and Ocean Salinity (SMOS) observations into the land surface model (LSM) Interaction Soil Biosphere Atmosphere (ISBA) at Météo-France. This study consists of an intercomparison experiment of different space-borne platforms providing surface soil moisture information [Advanced Microwave Scanning Radiometer for Earth Observing (AMSR-E) and European Remote Sensing Satellite Scatterometer (ERS-Scat)] with the reanalysis soil moisture predictions over France from the model suite of Système d’analyse fournissant des renseignements atmosphériques à la neige (SAFRAN), ISBA, and coupled model (MODCOU; SIM) of Météo-France for the years of 2003–05. Both modeled and remotely sensed data are initially validated against in situ observations obtained at the experimental soil moisture monitoring site Surface Monitoring of the Soil Reservoir Experiment (SMOSREX) in southwestern France. Two different AMSR-E soil moisture products are compared in the course of this study—the official AMSR-E product from the National Snow and Ice Data Center (NSIDC) and a new product developed at the Vrije Universiteit Amsterdam and NASA (VUA–NASA)—which were obtained using two different retrieval algorithms. This allows for an additional assessment of the different algorithms while using identical brightness temperature datasets. This study shows that a good correlation generally exists between AMSR-E (VUA–NASA), ERS-Scat, and SIM for low altitudes and low-to-moderate vegetation covers (1.5–3 kg m−2 vegetation water content), with a reduction in the correlation in mountainous regions. It also shows that the AMSR-E (NSIDC) soil moisture product has significant differences when compared to the other datasets.
Anticyclonic Eddies in the Alaskan Stream
Authors: Ueno Hiromichi , Sato Kanako , Freeland Howard J. , Crawford William R. , Onishi Hiroji , Oka Eitarou , Suga Toshio 
Published: September 2008

AbstractAnticyclonic eddies propagating southwestward in the Alaskan Stream (AS) were investigated through analysis of altimetry data from satellite observations during 1992–2006 and hydrographic data from profiling float observations during 2001–06. Fifteen long-lived eddies were identified and categorized based on their area of first appearance. Three eddies were present at the beginning of the satellite observations; another three formed in the eastern Gulf of Alaska off Sitka, Alaska; and four were first detected at the head of the Gulf of Alaska near Yakutat, Alaska. The other five eddies formed along the AS between 157° and 169°W, and were named AS eddies. While the eddies that formed in the Gulf of Alaska mainly decayed before exiting the Gulf of Alaska, the AS eddies mostly crossed the 180° meridian and reached the western subarctic gyre. Four of five AS eddies formed under negative or weakly positive wind stress curls, which possibly caused AS separation from the coast. Comparison of eddy propagation speeds in the AS with the bottom slope showed that eddies propagated faster over steeper slopes, although eddy speeds were slower than those predicted by the topographic planetary wave dispersion relation. An AS eddy was observed by profiling floats in the western subarctic gyre after it detached from the AS. Intermediate-layer water near the eddy center had low potential vorticity compared with the surrounding water, suggesting that AS eddies provided the western subarctic gyre with water just south of the Aleutian Islands.
Dynamics of the Wind-Driven Sea Level Variation around Antarctica
Authors: Kusahara Kazuya , Ohshima Kay I. 
Published: September 2008

Abstract
Coastal sea level variation around Antarctica is characterized by a coherent (circumpolarly in-phase) fluctuation, correlated with the Antarctic Oscillation (AAO). This study addresses the dynamics of the wind-driven sea level variation around Antarctica. A realistic barotropic numerical model reproduced well the observed sea level around Antarctica. From numerical model experiments, the authors demonstrate that the forcing responsible for the coastal sea level is the wind stress at the coastal boundary. Both the dominant coherent signal and westward propagating signals are identified in the model, and these signals are trapped over the shelf and slope around Antarctica. As a mechanism of these trapped signals, the authors consider analytical solutions of the oceanic response to alongshore wind stress over the shelf and slope in the circumpolar domain. In these solutions, besides the shelf wave mode, a wavenumber-zero mode appears and characterizes the coastal dynamics around Antarctica. At periods from 10 to 200 days, the coherent sea level can be explained quantitatively by the solution of this wavenumber-zero mode with a 5–10-day damping time scale. The spectral peaks of the westward propagating signals can be explained by the resonance of the shelf wave mode. The wavenumber-zero mode can respond to the wavenumber-zero forcing at any frequency and the degree of response increases with decreasing frequency. In addition, the wavenumber-zero component of wind stress, corresponding to the AAO variation, is a dominant forcing. Therefore, the coherent sea level variation around Antarctica is preferably generated and becomes a dominant feature in the circumpolar domain, particularly at lower frequencies.
Comparison of Methods Used to Generate Probabilistic Quantitative Precipitation Forecasts over South America
Authors: Ruiz Juan , Saulo Celeste , Kalnay Eugenia 
Published: September 2008

Abstract
In this work, the quality of several probabilistic quantitative precipitation forecasts (PQPFs) is examined. The analysis is focused over South America during a 2-month period in the warm season. Several ways of generating and calibrating the PQPFs have been tested, using different ensemble systems and single-model runs. Two alternative calibration techniques (static and dynamic) have been tested.
To take into account different precipitation regimes, PQPF performance has been evaluated over two regions: the northern part of South America, characterized by a tropical regime, and the southern part, where synoptic-scale forcing is stronger. The results support the adoption of such area separation, since differences in the precipitation regimes produce significant differences in PQPF performance.
The more skillful PQPFs are the ones obtained after calibration. PQPFs derived from the ensemble mean also show higher skill and better reliability than those derived from the single ensemble members. The performance of the PQPFs derived from both ensemble systems is similar over the southern part of the region; however, over the northern part the superensemble approach seems to achieve better results in both reliability and skill.
Finally, the impact of using Climate Prediction Center morphing technique (CMORPH) estimates to calibrate the precipitation forecast has been explored since the more extensive coverage of this dataset would allow its use over areas where the rain gauge coverage is insufficient. Results suggest that systematic biases present in the CMORPH estimates produce only a slight degradation of the resulting PQPF.
Metal vapour behaviour in thermal plasma of gas tungsten arcs during welding
Authors: Yamamoto K. , Tanaka M. , Tashiro S. , Nakata K. , Yamazaki K. , Yamamoto E. , Suzuki K. , Murphy A. B. 
Published: September 2008

A gas tungsten arc in helium and argon was modelled taking into account the contamination of the plasma by metal vapour from the weld pool. The whole region of gas tungsten arc atmosphere including the tungsten cathode, arc plasma and weld pool was treated using a unified numerical model. A viscosity approximation was used to express the diffusion coefficient in terms of viscosity of shielding gas and metal vapour. The transient two-dimensional distributions of the temperature, velocity of plasma flow and iron vapour concentration were predicted, together with the weld penetration as a function of time for a 150 A arc current at the atmospheric pressure, both for helium and argon welding gases. It was shown that the thermal plasma in gas tungsten arcs is influenced by iron vapour from the weld pool surface and that the concentration of iron vapour in plasma is dependent on the temperature of the weld pool.
Role of hypoxia-inducible factor 1alpha in the integrity of articular cartilage in murine knee joints
Authors: Gelse Kolja , Pfander David , Obier Simon , Knaup Karl X , Wiesener Michael , Hennig Friedrich F , Swoboda Bernd 
Published: September 2008

Abstract
Introduction
Chondrocytes have to withstand considerable hypoxic conditions within the avascular articular cartilage. The present study investigated the effects of inhibiting or stabilizing hypoxia-inducible factor (HIF)-1α by 2-methoxyestradiol or dimethyloxaloylglycine on the progression of osteoarthritis in murine knee joints.

Methods
2-Methoxyestradiol was injected six times over a period of 2 weeks into the left knee joint of Balb/C mice. Joints were assessed by histochemical and immunohistochemical methods, 3 weeks and 12 weeks following the first injection. Dimethyloxaloylglycine, an inhibitor of HIF-degrading prolyl-hydroxylases, was injected into the left knee joints of STR/ORT mice once a week over the entire period of 12 weeks. Right knee joints that received a saline solution served as controls. In addition, the effects of dimethyloxaloylglycine on HIF-1 target gene expression and on collagen metabolism were analyzed in vitro.

Results
Injection of 2-methoxyestradiol led to osteoarthritic changes in the treated knee joints of Balb/C mice. The first signs of osteophyte formation were observed in the knee joints after 3 weeks, followed by progressive destruction of the articular cartilage at 12 weeks that was not, however, accompanied by inflammatory reactions. Injection of dimethyloxaloylglycine could not prevent severe osteoarthritis that spontaneously developed in the knee joints of STR/ORT mice. In chondrocyte cultures, administration of dimethyloxaloylglycine resulted in an upregulation of Sox9 expression. Such a stimulatory effect was not observed, however, for the expression of type II collagen, which might be the indirect consequence of intracellular collagen retention observed by immunofluorescence or of increased expression of IL-1β and IL-6.

Conclusions
Induction of osteoarthritis by 2-methoxyestradiol demonstrates the importance of HIF-1 in maintaining the integrity of hypoxic articular cartilage. Stabilization of HIF-1 by dimethyloxaloylglycine, however, was not of therapeutic value, since this nonselective prolyl-hydroxylase inhibitor also interferes with proper collagen metabolism and induces the expression of catabolic cytokines