bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of serum TM, PAF, and CD62P in patients with autologous arteriovenous fistula and the correlation with vascular access function
Authors: Jiang Yan , Liu Zongyang , Liu Liting , Xiong Zhiqian , Chen Yan , Zhang Shuai , Su Chaojiang 
Published: March 2024

AbstractBackgroundEnd‐stage renal disease (ESRD) is the final stage of chronic kidney disease (CKD).AimsWe aimed to analyze the expression differences of serum thrombomodulin (TM), platelet‐activating factor (PAF), and P‐selectin (CD62P) in patients with autologous arteriovenous fistula (AVF) and the correlation with vascular access function.MethodsThe case data were retrospectively analyzed. Moreover, 160 patients with AVF maintenance hemodialysis were selected as the AVF group, and 150 healthy participants were selected as the healthy control group. According to the function of vascular access, patients in the AVF group were divided into Group A (n = 50, after the first establishment of AVF), Group B (n = 64, normal vascular access function after hemodialysis treatment), and Group C (n = 46, vascular access failure). Pearson analysis was conducted to explore the correlation between serum TM, PAF, CD62P content, and vascular pathological examination indicators, to evaluate the value of TM, PAF, and CD62P levels in predicting vascular access failure in patients with AVF.Results and DiscussionThe serum levels of TM, PAF, and CD62P were positively correlated with the expressions of CD68 and MCP‐1, respectively (p < .001). Serum TM was positively correlated with the levels of PAF and CD62P (p < .001), and PAF was positively correlated with the levels of CD62P (p < .001), respectively. Serum levels of TM, PAF and CD62P were risk factors for vascular access failure in AVF patients (p < .05). The area under the curve of serum TM, PAF and CD62P levels in predicting vascular access failure in AVF patients was 0.879.ConclusionThe serum levels of TM, PAF, and CD62P in AVF patients were correlated with the vascular access function of AVF patients, which was very important for maintaining the stability of vascular access function, and had certain value in predicting vascular access failure/disorder in AVF patients, and could be popularized and applied.
Prediagnostic use of menopausal hormone therapy and long‐term survival of localized epithelial ovarian cancer: The Extreme study
Authors: Duus Alberte Hjorth , Hannibal Charlotte Gerd , Baandrup Louise , Zheng Guoqiao , Galanakis Michael , Maltesen Thomas , Hertzum‐Larsen Rasmus , Mørch Lina S. , Kjær Susanne K. 
Published: March 2024

AbstractUse of menopausal hormone therapy (MHT) prior to an epithelial ovarian cancer (EOC) diagnosis has been suggested to be associated with improved survival. In a recent nationwide cohort study, we found that prediagnostic long‐term MHT use, especially estrogen therapy (ET), was associated with improved long‐term survival in women with nonlocalized EOC. Our aim was to investigate the influence of prediagnostic MHT use on long‐term survival among women with localized EOC in the same nationwide study. Our study cohort comprised all women aged 50 years or older with an EOC diagnosis in Denmark 2000–2014 (n = 2097) identified from the Extreme study. We collected information on usage of systemic ET and estrogen plus progestin therapy (EPT) from the Danish National Prescription Registry. By using pseudo‐values, 5‐ and 10‐year absolute and relative survival probabilities were estimated with 95% confidence intervals (CIs) while adjusting for histology, comorbidity, and income. Relative survival probabilities >1 indicate better survival. The 5‐year absolute survival probabilities were 61% and 56%, respectively, among women who were nonusers and users of prediagnostic MHT, whereas these numbers were 46% and 41%, respectively, regarding 10‐year survival. Use of MHT was not significantly associated with an improved 5‐ or 10‐year survival in women with localized EOC (5‐year relative survival probability = 0.95, 95% CI: 0.89–1.02; 10‐year relative survival probability = 0.92, 95% CI: 0.84–1.02). Similar findings were seen for systemic ET or EPT use. Our findings do not suggest a positive benefit from prediagnostic MHT use on long‐term survival of localized EOC.
An intelligent self‐powered life jacket system integrating multiple triboelectric fiber sensors for drowning rescue
Authors: Zhang Yiping , Li Chengyu , Wei Chuanhui , Cheng Renwei , Lv Tianmei , Wang Junpeng , Zhao Cong , Wang Zhaoyang , Li Fangming , Peng Xiao , Xu Minyi , Dong Kai 
Published: March 2024

AbstractThe inherent unpredictability of the maritime environment leads to low rates of survival during accidents. Life jackets serve as a crucial safety measure in underwater environments. Nonetheless, most conventional life jackets lack the capability to monitor the wearer's underwater body movements, impeding their effectiveness in rescue operations. Here, we present an intelligent self‐powered life jacket system (SPLJ) composed of a wireless body area sensing network, a set of deep learning analytics, and a human condition detection platform. Six coaxial core‐shell structure triboelectric fiber sensors with high sensitivity, stretchability, and flexibility are integrated into this system. Additionally, a portable integrated circuit module is incorporated into the SPLJ to facilitate real‐time monitoring of the wearer's movement. Moreover, by leveraging the deep‐learning‐assisted data analytics and establishing a robust correlation between the wearer's movements and condition, we have developed a comprehensive system for monitoring drowning individuals, achieving an outstanding recognition accuracy of 100%. This groundbreaking work introduces a fresh approach to underwater intelligent survival devices, offering promising prospects for advancing underwater smart wearable devices in rescue operations and the development of ocean industry.image
Suitability of Fourier transform infrared microscopy for the diagnosis of cystic echinococcosis in human tissue sections
Authors: Brunner A. , Unterberger S. H. , Auer H. , Hautz T. , Schneeberger S. , Stalder R. , Badzoka J. , Kappacher C. , Huck C. W. , Zelger B. , Pallua J. D. 
Published: March 2024

AbstractCystic echinococcosis (CE) is a global health concern caused by cestodes, posing diagnostic challenges due to nonspecific symptoms and inconclusive radiographic results. Diagnosis relies on histopathological evaluation of affected tissue, demanding comprehensive tools. In this retrospective case study, Fourier transform infrared microscopy was explored for detecting and identifying CE through biochemical changes in human tissue sections. Tissue samples from 11 confirmed CE patients were analyzed. Archived FFPE blocks were cut and stained, and then CE‐positive unstained sections were examined using Fourier transform infrared microscopy post‐deparaffinization. Results revealed the method's ability to distinguish echinococcus elements from human tissue, irrespective of organ type. This research showcases the potential of mid‐infrared microscopy as a valuable diagnostic tool for CE, offering promise in enhancing diagnostic precision in the face of the disease's complexities.
Saliency enhancement method for photoacoustic molecular imaging based on Grüneisen relaxation nonlinear effect
Authors: Wang Xiatian , Xie Zhihua , Lin Riqiang , Shu Chengyou , Lv Shengmiao , Guo Pengkun , Xu Haoxing , Zhang Jinke , Dong Liquan , Gong Xiaojing 
Published: March 2024

AbstractPhotoacoustic molecular imaging technology has a wide range of applications in biomedical research. In practical scenarios, both the probes and blood generate signals, resulting in the saliency of the probes in the blood environment being diminished, impacting imaging quality. Although several methods have been proposed for saliency enhancement, they inevitably suffer from moderate generality and detection speed. The Grüneisen relaxation (GR) nonlinear effect offers an alternative for enhancing saliency and can improve generality and speed. In this article, the excitation and detection efficiencies are optimized to enhance the GR signal amplitude. Experimental studies show that the saliency of the probe is enhanced. Moreover, the issue of signal aliasing is studied to ensure the accuracy of enhancement results in the tissues. In a word, the feasibility of the GR‐based imaging method in saliency enhancement is successfully demonstrated in the study, showing the superiorities of good generality and detection speed.
Silencing of circ_0088036 inhibits growth and invasion of lung adenocarcinoma through miR‐203/SP1 axis
Authors: Liu Xiuhua , Feng Yan , Wang Linna , Shi Lei , Ji Kunxiang , Hu Nan , Du Yang , Liu Mingyang , Wang Man 
Published: March 2024

AbstractLung cancer is the leading cause of cancer‐related deaths worldwide. Circular RNA (circRNA) circ_0088036 is a recently discovered circRNA known for its roles in rheumatoid arthritis. The study aimed to study the function of circ_0088036 in lung adenocarcinoma (LUAD). Circ_0088036 expressions were analyzed in the Gene Expression Omnibus (GEO) database. The relationship between circ_0088036 expressions and clinicopathological data of LUAD was assessed. The messenger RNA and protein levels were analyzed by quantitative real‐time polymerase chain reaction and Western blot. Cell viability, apoptosis, and invasion were tested by Cell Counting Kit‐8, flow cytometry, and transwell assay. The direct interaction between microRNA‐203 (miR‐203) and circ_0088036 or specificity protein 1 (SP1) was confirmed by dual‐luciferase reporter assay, RNA pull‐down, and RNA immunoprecipitation assays. Circ_0088036 was overexpressed in LUAD from the analysis of the GEO database. The poor prognosis was found in the patients with high expressions of circ_0088036. The level of Circ_0088036 was increased in LUAD tissues and cells. In terms of function, the deletion of circ_0088036 inhibited LUAD tumorigenesis in vitro by repressing cell growth, invasion, and epithelial–mesenchymal transition (EMT). In mechanism, circ_0088036 could competitively sponge miR‐203, thereby affecting the expressions of the target gene SP1. In addition, lessening of miR‐203 and enlarging of SP1 could eliminate the anticancer effect of short hairpin RNA‐circ_0088036 on LUAD cells. Besides, the knockout of circ_0088036 hindered the growth of xenografted tumors in vivo. Circ_0088036 promoted the LUAD cell growth, invasion, and EMT via modulating the miR‐203/SP1 axis in LUAD.
Unraveling Hypothalamus‐Pituitary dysregulation: Hypergonadotropism in F1 progeny due to prenatal exposure to hexavalent chromium
Authors: Navin Ajit Kumar , Aruldhas Mariajoseph Michael , Mani Kathiresh Kumar , Navaneethabalakrishnan Shobana , Venkatachalam Sankar , Banu Sakhila K. 
Published: March 2024

AbstractThe endocrine disruptor hexavalent chromium [Cr(VI)] is a proven reproductive toxicant. We recently demonstrated that prenatal Cr(VI) exposure causes testicular resistance to gonadotropins, resulting in hypergonadotropic hypoandrogenism in F1 rats. However, the mechanism driving hypergonadotropism in F1 rats exposed to Cr(VI) prenatally remains an enigma. Therefore, we hypothesized that ‘Prenatal Cr(VI) exposure may disrupt steroid hormones‐mediated negative feedback regulation of the hypothalamic GnRH, and its receptor in the pituitary of F1 rats, leading to hypergonadotropism.’ We administered potassium dichromate (50, 100, or 200 mg/L) to pregnant rats through drinking water between days 9 and 14, and their male F1 offspring were euthanized at 60 days of age. Prenatal Cr(VI) exposure in F1 rats resulted in the accumulation of Cr in the hypothalamus and pituitary. Western blot detected decreased hypothalamic GnRH, Kisspeptin1, and its receptor GPR54, along with diminished ERα, AR, aromatase, and 5α reductase, and GnRH regulatory transcription factors Pit‐1 and GATA‐4 proteins. Immunohistochemical studies revealed increased immunopositivity of GnRH receptor, AR, 5α reductase, ERα, ERβ, and aromatase proteins in the pituitary, whereas decreased Kisspeptin1, GPR54, and inhibin β. Our findings imply that Cr(VI) exposure during the prenatal period disrupts the hypothalamic Kisspeptin‐GPR54‐Pit‐1/GATA4‐GnRH network, boosting the pituitary GnRH receptor. We conclude that prenatal exposure to Cr(VI) alters GnRH expression in the hypothalamus and its receptor in the pituitary of F1 progeny through interfering with the negative feedback effect of androgens and estrogens.
Lack of vitamin D signalling in mesenchymal progenitors causes fatty infiltration in muscle
Authors: Hosoyama Tohru , Kawai‐Takaishi Minako , Iida Hiroki , Yamamoto Yoko , Nakamichi Yuko , Watanabe Tsuyoshi , Takemura Marie , Kato Shigeaki , Uezumi Akiyoshi , Matsui Yasumoto 
Published: March 2024

AbstractBackgroundRecent studies have indicated the importance of muscle quality in addition to muscle quantity in sarcopenia pathophysiology. Intramuscular adipose tissue (IMAT), which originates from mesenchymal progenitors (MPs) in adult skeletal muscle, is a key factor affecting muscle quality in older adults, suggesting that controlling IMAT formation is a promising therapeutic strategy for sarcopenia. However, the molecular mechanism underlying IMAT formation in older adults has not been clarified. We recently found that the vitamin D receptor (VDR) is highly expressed in MPs in comparison to myotubes (P = 0.028, N = 3), indicating a potential role of vitamin D signalling in MPs. In this study, we aimed to clarify the role of vitamin D signalling in MP kinetics, with a focus on adipogenesis.MethodsMPs isolated from mouse skeletal muscles were subjected to adipogenic differentiation conditions with or without vitamin D (1α,25(OH)2D3, 100 nM) for 7 days, and adipogenicity was evaluated based on adipogenic marker expression. For in vivo analysis, tamoxifen‐inducible MP‐specific VDR‐deficient (VdrMPcKO) mice were newly developed to investigate whether lack of vitamin D signalling in MPs is involved in IMAT formation. To induce muscle atrophy, VdrMPcKO male mice were subjected to tenotomy of the gastrocnemius muscle, and then muscle weight, myofibre cross‐sectional area, adipogenic marker expression, and fatty infiltration into the muscle were evaluated at 3 weeks after operation (N = 3–4). In addition, a vitamin D‐deficient diet was provided to wild‐type male mice (3 and 20 months of age, N = 5) for 3 months to investigate whether vitamin D deficiency causes IMAT formation.ResultsVitamin D treatment nearly completely inhibited adipogenesis of MPs through Runx1‐mediated transcriptional modifications of early adipogenic factors such as PPARγ (P = 0.0031) and C/EBPα (P = 0.0027), whereas VDR‐deficient MPs derived from VdrMPcKO mice differentiated into adipocytes even in the presence of vitamin D (P = 0.0044, Oil‐Red O+ area). In consistency with in‐vitro findings, VdrMPcKO mice and mice fed a vitamin D‐deficient diet exhibited fat deposition in atrophied (P = 0.0311) and aged (P = 0.0216) skeletal muscle, respectively.ConclusionsVitamin D signalling is important to prevent fate decision of MPs towards the adipogenic lineage. As vitamin D levels decline with age, our data indicate that decreased vitamin D levels may be one of the causes of IMAT formation in older adults, and vitamin D signalling may be a novel therapeutic target for sarcopenia.
The future of faculty recruitment: Inspiring students into an academic career
Authors: Wong Cynthia L. , Melo Mary Anne S. , Scarpa‐Lota Nathalie , Moazzez Rebecca , Furness Alan , Stewart Kelton T. 
Published: March 2024

AbstractThe dilemma surrounding faculty shortages within dental education continues to present significant challenges for the dental profession. There remains a tremendous need to create an effective and sustainable pathway for the recruitment of faculty into dental academia, with an emphasis on the establishment of a more diverse and representative faculty composition. This perspective paper proposes a blueprint to nurture and inspire dental students into academia.
Altered glycosylation in secreting cells of the gastric glands of aquaporin‐4‐deficient mice
Authors: Mentino Donatella , Nicchia Grazia Paola , Frigeri Antonio , Desantis Salvatore , Guglielmi Marco Vito , Semeraro Daniela , Scillitani Giovanni , Mastrodonato Maria 
Published: March 2024

AbstractAquaporins (AQPs) are important for water transport in the gastrointestinal tract. Changes in their expression and/or localization could cause in disorders and be used as therapeutic targets. Aquaporin‐4 (AQP4) is expressed predominantly on the basolateral membrane of the parietal cells in the corpus of the murine gastric glands. Although the secretion of gastric juice is not affected in AQP4‐deficient knockout, we evaluated by light microscopy whether the lack of AQP4 affects the glycopatterns of secreting gastric cells. Wild type (WT) and AQP4‐deficient knockout mice (KO) were fed a standard diet ad libitum before sacrifice. Segments of stomach corpus were collected, fixed in buffered formalin, and embedded in paraffin wax. Sections, 5‐μm thick, were analyzed by histochemical methods (Periodic acid‐Schiff, Alcian Blue pH 2.5), and binding of lectins specific to GalNAc (SBA, DBA), Gal (PNA) GlcNAc (WGA, GSAII) mannose and/or glucose (ConA), and fucose (UEA‐I, AAA, LTA). Immunohistochemical methods such as anti‐Muc6 for neck cells and anti‐ β‐ H+/K+‐ATPase for parietal cells were also performed. Compared to WT mice, in the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated, and fucosylated residues were observed; lower fucosylation resulted also in the parietal cells. The observed differences of KO in respect to WT could lead to severer pathological conditions.Research Highlights
Glycopatterns in gastric glands were compared between wild type (WT) and AQP4‐deficient knockout (KO) mice by histochemical and lectin‐binding methods.
In the mucous cells of KO lower amounts of glycans with galactosyl/galactosaminylated, glycosyl/glycosaminylated and fucosylated residues were observed.
In the parietal cells lower fucosylation also resulted.
AQP4‐deficiency affects glycosylation and could result in altered functionality and pathological conditions.
Nationwide implementation of lenalidomide maintenance in multiple myeloma: A retrospective, real‐world study
Authors: Harsløf Mads , Chanchiri Iman , Silkjær Trine , Frølund Ulf Christian , Teodorescu Elena Manuela , Nielsen Kristina Buchardi , Nielsen Per Ishøy , Pedersen Per Trøllund , Iversen Katrine Fladeland , Lund Thomas , Grønbæk Kirsten , Thorsteinsdottir Sigrun , Vangsted Annette , Szabo Agoston Gyula 
Published: March 2024

AbstractLenalidomide maintenance (LM) has shown benefit in progression‐free survival (PFS) and overall survival (OS) in clinical trials. LM is the recommended standard of care in patients with newly diagnosed multiple myeloma (MM) after high‐dose melphalan and autologous stem cell transplantation (HDM‐ASCT). In Denmark, LM has been approved and publicly funded for all patients treated with HDM‐ASCT since June 2019. Patients with newly diagnosed MM treated with their first HDM‐ASCT between June 2019 and March 2022 were included and followed until data cut‐off in June 2023. To compare outcomes, a historical pre‐LM cohort from the Danish MM Registry, consisting of 364 MM patients treated with HDM‐ASCT between June 2015 and June 2019, was used. Among 364 patients treated with HDM‐ASCT after June 2019, 22.3% received consolidation therapy and 3.7% underwent tandem HDM‐ASCT. During follow‐up, 297 patients (81.6%) initiated maintenance therapy, with 277 (76.1%) receiving LM. Overall, 145 patients (52.3%) discontinued LM most commonly due to toxicity 75 (51.7%), with fatigue (30.7%), cytopenia (25.3%), and neuropathy (17.3%) being the main reasons. In a 6‐month landmark analysis, early discontinuation did not negatively impact PFS or OS. The LM cohort had similar PFS, and OS compared to the pre‐LM cohort. The 3‐year PFS and OS rates in the LM cohort were 61% and 86%, respectively, while the pre‐LM cohort had a 3‐year PFS of 55% and a 3‐year OS of 89%. In conclusion, the introduction of LM as a nationwide treatment option in Denmark did not lead to improved clinical outcomes.
Vascular stenting of a malignant arterial blowout as a bridge to effective systemic and regional therapy
Authors: Williams Taylor P. , Cox Mitchell W. , Jana Bagi , Ross Merrick I. , Fisher Sarah B. , Walker John P. , Hume Celyne B. , Mayo Lauren L. , Tyler Douglas S. 
Published: March 2024

AbstractLocally advanced cutaneous squamous cell carcinoma can erode into blood vessels, leading to vascular blowout, requiring emergent surgical intervention. We describe a first case of this disease complication which was effectively managed with endovascular stenting as a bridge to effective systemic and regional therapy. We discuss the efficacy of this staged approach which is novel and timely in a clinical environment of increasingly effective systemic therapies.
Recycled bone grafts treated with extracorporeal irradiation or liquid nitrogen freezing after malignant tumor resection
Authors: Takeuchi Yuta , Sakamoto Akio , Noguchi Takashi , Toguchida Junya , Matsuda Shuichi 
Published: March 2024

AbstractIntroductionRecycled bone autografts prepared using extracorporeal irradiation (ECIR) or liquid nitrogen freezing (LNF) methods have been used for the reconstruction of skeletal elements after wide resection of sarcomas involving bone tissues. Few reports include long‐term follow‐up data for histological analyses of recycled autografts, particularly in the case of ECIR autografts.MaterialsA total of 34 malignant bone and soft tissue tumors were resected and reconstructed using 11 ECIR‐ and 23 LNF‐recycled autografts; the mean postoperative follow‐ups were 14 and 8 years, respectively. ECIR was used for either osteosarcomas or Ewing sarcomas, whereas in addition to these tumors LNF was used for chondrosarcomas and soft tissue sarcomas involving bone tissues. Recycled bone was implanted as total bone, osteoarticular, or intercalary grafts, with or without prosthesis or vascularized fibular grafts.ResultsThe 10‐year graft survival rate was similar between groups, 81.8% using ECIR and 70.2% using LNF. There were no autograft‐related tumor recurrences in either group. Graft survival was unrelated to type of graft or additional procedures. Complication rates tended to be higher using ECIR (64%) compared with LNF (52%) and the infection rate was significantly higher with ECIR (27%) versus LNF (0%). At the final assessment, plain radiographs revealed original recycled bone was present in 7 of 11 ECIR cases and in zero cases treated with LNF autografts, indicating that recycled bone treated with LNF autografts was remodeled into new bone. Histological examination of ECIR‐treated bones revealed a delayed and incomplete endochondral ossification process, necrosis and empty lacunae. Conversely, LNF autografts showed remodeled bones with normal trabecular structures.ConclusionsECIR and LNF treatment of autografts provided adequate tumor control with acceptable clinical results as a reconstruction method.