bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tocopherols by Hydride Reduction of Dialkylamino Derivatives
Authors: Netscher Thomas , Mazzini Francesco , Jestin Roselyne 
Published: January 2007

AbstractAminomethylation with Mannich reagents derived from secondary amines and paraformaldehyde under improved conditions has been used to convert non‐α‐tocopherol homologues into α‐tocopherol, the biologically most important vitamin E compound. Mono‐ and bis(aminomethylated) β‐, γ‐ and δ‐tocopherol were then subsequently transformed into the corresponding tocopherols (α‐ and β‐tocopherol) by reductive deamination. As an alternative to classical catalytic hydrogenation in the last step, efficient laboratory protocols using complex hydrides have been derived and applied to the preparation of labelled vitamin E compounds.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Bioactive Natural Products from the Endophytic Fungus Ascochyta sp. from Meliotus dentatus – Configurational Assignment by Solid‐State CD and TDDFT Calculations
Authors: Krohn Karsten , Kock Ines , Elsässer Brigitta , Flörke Ulrich , Schulz Barbara , Draeger Siegfried , Pescitelli Gennaro , Antus Sándor , Kurtán Tibor 
Published: January 2007

AbstractTwo new metabolites, (4S)‐(+)‐ascochin (1a) and (S,S)‐(+)‐ascodiketone (3), together with the known compounds (3R,4R)‐(–)‐4‐hydroxymellein (2), ent‐α‐cyperone (4) and (3S,4R)‐(–)‐dihydroxy‐(6S)‐undecyl‐α‐pyranone (5) were isolated from cultures of the of the endophytic fungus Ascochyta sp. The biologically active isocoumarin derivative, (4S)‐(+)‐ascochin (1a), has an unusual substitution pattern which was confirmed by X‐ray diffraction. Its absolute configuration was determined by our solid‐state TDDFT CD methodology using the X‐ray coordinates as input for the calculation. By catalytic hydrogenation, (4S)‐(+)‐ascochin was converted into the corresponding (3S,4S)‐dihydroisocoumarin derivative 1b. The measured and TDDFT calculated CD spectra enabled studies on the correlation between absolute configuration and n‐π* transition Cotton effect. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Enhanced electrophoretic resolution of monosulfate glycosaminoglycan disaccharide isomers on poly(methyl methacrylate) chips
Authors: Zhang Yong , Ping Guichen , Zhu Bingmei , Kaji Noritada , Tokeshi Manabu , Baba Yoshinobu 
Published: January 2007

AbstractTo improve the separation of monosulfate glycosaminoglycan disaccharide isomers by microchip electrophoresis, we found that addition of 1,4‐dioxane (DO) dramatically improved analyte resolution, probably due to solvation effects. Methylcellulose (MC) was tested for the ability to suppress EOF and analyte adsorption to the chip. To improve analyte resolution, buffer pH, β‐CD, and DO were systematically investigated. Fast separation was achieved by increasing the electric field strength, and field‐amplified sample stacking occurred with increasing buffer concentrations. Therefore, based on our findings, we describe an efficient method for the separation of monosulfate and trisulfate unsaturated disaccharides (ΔDi‐UA2S, ΔDi‐4S, ΔDi‐6S, and ΔDi‐triS) derivatized with 2‐aminoacridone hydrochloride. A mixture of monosulfate disaccharide isomers (ΔDi‐UA2S, ΔDi‐4S, and ΔDi‐6S) was baseline‐separated within 75 s on a poly(methyl methacrylate) chip using a mixed buffer (DO/running buffer 57:43 v:v), 0.5% MC, pH 6.81, with an Esep of 558 V/cm. The theoretical plate was in the range of 5×105 to 1×106 m−1.
A non‐baseline algorithm for damage locating in flexural structures using dynamic distributed macro‐strain responses
Authors: Li S. Z. , Wu Z. S. 
Published: January 2007

AbstractThe concept of distributed strain‐sensing techniques has been proposed in our recent research, which was dedicated to utilizing the strain distributions throughout the full or partial areas of structures to detect arbitrary and unforeseen damage. An algorithm not requiring a detailed analytical model is presented for damage locating in flexural structures through the direct use of dynamic responses recorded by distributed long‐gauge strain sensors. The modal macro‐strain vector (MMSV), which has been proven to have a mapping relation with displacement mode shape, can be extracted directly from macro‐strain time‐series data, from which a damage evaluating index can be derived and used as an indicator for locating damage. Numerical examples are simulated to verify the sensitivity and effectiveness of the index in different cases. Furthermore, experimental investigations on a cantilevered beam with various long‐gauge fibre optic sensors placements are carried out to examine the feasibility and applicability of the proposed method. Copyright © 2007 John Wiley & Sons, Ltd.
Type I error rates in association versus joint linkage/association tests in related individuals
Authors: Kent Jack W. , Dyer Thomas D. , Göring Harald H. H. , Blangero John 
Published: January 2007

AbstractPositional gene discovery on pedigree data typically involves initial gross localization by linkage analysis with subsequent finer localization by association analysis in areas that show evidence of linkage. We examine the effect of including linkage information when testing for association in the context of variance‐components‐based pedigree analysis. We present simulation experiments showing that, at least in the extreme case of a rare private allele, failing to include the linkage variance component in the association model results in excessive Type I error that increases with allele copy number and/or quantitative trait locus (QTL) effect size. Joint estimation of the linkage variance component in the association model reduces Type I error to nominal expectations. This holds whether allele‐sharing probabilities are estimated from a polymorphic marker or from the very single‐nucleotide polymorphism (SNP) being tested for association, although the latter provides much less power. These results support the idea that an appropriate association analysis must test both the random effect of shared marker alleles (linkage) and the mean effects of the marker genotypes (association). Genet. Epidemiol. 2007. © 2007 Wiley‐Liss, Inc.
Dual regulation of astrocyte gap junction hemichannels by growth factors and a pro‐inflammatory cytokine via the mitogen‐activated protein kinase cascade
Authors: Morita Mitsuhiro , Saruta Chihiro , Kozuka Nagisa , Okubo Yoshiumi , Itakura Makoto , Takahashi Masami , Kudo Yoshihisa 
Published: January 2007

AbstractEvidence that glutamate and ATP release from astrocytes can occur via gap junction hemichannels (GJHCs) is accumulating. However, the GJHC is still only one possible release mechanism and has not been detected in some studies, although this may be because the levels were below those detectable by the system used. Because of these conflicting results, we hypothesized that release from astrocyte GJHCs might depend on different astrocyte states, and screened for factors affecting astrocyte GJHC activity by measuring fluorescent dye leakage via GJHCs using a conventional method for GJHC acivation, i.e. removal of extracellular divalent cations. Astrocytes cultured in Dulbecco's minimal essential medium containing 10% fetal calf serum, a medium widely used for astrocyte studies, did not show dye leakage, whereas those cultured in a defined medium showed substantial dye leakage, which was confirmed pharmacologically to be due to GJHCs and not to P2x7 receptors. EGF and bFGF inhibited the GJHC activity via the mitogen‐activated protein kinase cascade, and the effect of the growth factors was reversed by interleukin‐1β. These factors altered GJHC activity within 10 min, but did not affect connexin 43 expression. GJHC activity in hippocampal slice culture preparations was measured using the same methods and found to be regulated in a similar manner. These results indicate that astrocyte GJHC activity is regulated by brain environmental factors. © 2007 Wiley‐Liss, Inc.
Suppressing phosphatidylcholine‐specific phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells
Authors: Wang Nan , Xie Kun , Huo Siwei , Zhao Jing , Zhang Shangli , Miao Junying 
Published: January 2007

AbstractIn the previous research, we found that D609 (tricyclodecan‐9‐yl‐xanthogenate) could induce human marrow stromal cell (hMSC) differentiation to neuron‐like cells. In this study, to understand the possible mechanism, we sequentially investigated the changes of phosphatidylcholine‐specific phospholipase C (PC‐PLC) activity, the expression of Rb, the intracellular reactive oxygen species (ROS) levels, NADPH oxidase and superoxide dismutase (SOD) activities when D609 induced neuronal differentiation in rat mesenchymal stem cells (MSCs). The results showed that D609 obviously inhibited the activity of PC‐PLC when it induced neuronal differentiation in rat MSCs. Simultaneously, ROS level and the activity of NADPH oxidase increased significantly, but the MnSOD and Cu/ZnSOD activities were not altered. Furthermore, the level of Rb protein was evidently elevated. Our data suggested that PC‐PLC mediated neuronal differentiation of rat MSCs by elevating NADPH oxidase activity, ROS level, and up‐regulating the expression of Rb protein. J. Cell. Biochem. 100: 1548–1557, 2007. © 2007 Wiley‐Liss, Inc.
TGF‐β1 calcium signaling in osteoblasts
Authors: Nesti Leon J. , Caterson E.J. , Li Wan‐Ju , Chang Richard , McCann Thane D. , Hoek Jan B. , Tuan Rocky S. 
Published: January 2007

AbstractTransforming growth factor‐β1 (TGF‐β1) action is known to be initiated by its binding to multiple cell surface receptors containing serine/threonine kinase domains that act to stimulate a cascade of signaling events in a variety of cell types. We have previously shown that TGF‐β1 and BMP‐2 treatment of primary human osteoblasts (HOBs) enhances cell‐substrate adhesion. In this report, we demonstrate that TGF‐β1 elicits a rapid, transient, and oscillatory rise in the intracellular Ca2+ concentration, [Ca2+]i, that is necessary for enhancement of cell adhesion in HOBs but does not alter the phosphorylation state of Smad proteins. This rise in [Ca2+]i in HOB is not observed in the absence of extracellular calcium or when the cells are treated with the L‐type Ca2+ channel blocker, nifedipine, but is stimulated upon treatment with the L‐type Ca2+ channel agonist, Bay K 8644, or under high K+ conditions. The rise in [Ca2+]i is severely attenuated after treatment of the cells with thapsigargin, a selective endoplasmic reticulum Ca2+ pump inhibitor. TGF‐β1 enhancement of HOB adhesion to tissue culture polystyrene is also inhibited in cells treated with nifedipine. These data suggest that intracellular Ca2+ signaling is an important second messenger of the TGF‐β1 signal transduction pathway in osteoblast function. J. Cell. Biochem. 101: 348–359, 2007. © 2007 Wiley‐Liss, Inc.
Oct‐4, Rex‐1, and Gata‐4 expression in human MSC increase the differentiation efficiency but not hTERT expression
Authors: Roche Stéphane , Richard Marie‐Jeanne , Favrot Marie‐Christine 
Published: January 2007

AbstractMicro‐environment seems to exert an important influence on human mesenchymal stem cell (MSC) differentiation and proliferative capacity in bone marrow as well as in culture ex vivo. Oct‐4, Rex‐1, and TERT genes are well‐known for the maintenance of pluripotentiality differentiation and the proliferative capacity of embryonic stem cells. Some previous data report expression of these embryonic factors in selected clones from bone marrow adult stem cells. Our goal was to study expression of Oct‐4, Rex‐1, and TERT in primary cultured human MSC according to the serum concentration. In addition, we have studied the expression of Gata‐4 since this factor plays a key role in organogenesis. We hypothesized that low serum concentration with appropriate growth factors may induce an undifferentiated status with a re‐expression of embryonic factors and extend differentiation capacity. Thus, using a defined culture medium, we report on the increased expression of Oct‐4, Rex‐1, and Gata‐4 in human MSC. We have correlated this expression to an increase in differentiation efficiency towards osteogenic and adipogenic phenotypes. Our data suggest that the culture medium used permits the emergence of adult stem cells with a high differentiation capacity and expression of embryonic factors. These cells may have important implications for cell therapy. J. Cell. Biochem. 101: 271–280, 2007. © 2007 Wiley‐Liss, Inc.
Fibrosis and cancer: Do myofibroblasts come also from epithelial cells via EMT?
Authors: Radisky Derek C. , Kenny Paraic A. , Bissell Mina J. 
Published: January 2007

AbstractMyofibroblasts produce and modify the extracellular matrix (ECM), secrete angiogenic and pro‐inflammatory factors, and stimulate epithelial cell proliferation and invasion. Myofibroblasts are normally induced transiently during wound healing, but inappropriate induction of myofibroblasts causes organ fibrosis, which greatly enhances the risk of subsequent cancer development. As myofibroblasts are also found in the reactive tumor stroma, the processes involved in their development and activation are an area of active investigation. Emerging evidence suggests that a major source of fibrosis‐ and tumor‐associated myofibroblasts is through transdifferentiation from non‐malignant epithelial or epithelial‐derived carcinoma cells through epithelial‐mesenchymal transition (EMT). This review will focus on the role of EMT in fibrosis, considered in the context of recent studies showing that exposure of epithelial cells to matrix metalloproteinases (MMPs) can lead to increased levels of cellular reactive oxygen species (ROS) that stimulate transdifferentiation to myofibroblast‐like cells. As deregulated MMP expression and increased cellular ROS are characteristic of both fibrosis and malignancy, these studies suggest that increased MMP expression may stimulate fibrosis, tumorigenesis, and tumor progression by inducing a specialized EMT in which epithelial cells transdifferentiate into activated myofibroblasts. This connection provides a new perspective on the development of the fibrosis and tumor microenvironments. J. Cell. Biochem. 101: 830–839, 2007. © 2007 Wiley‐Liss, Inc.
Functions and regulation of human artemis in double strand break repair
Authors: Dahm Kirsten 
Published: January 2007

AbstractCells, which lacked the activity of the nuclease Artemis, retained approximately 10% of unrepaired double strand breaks (DSBs) at later timepoints after ionizing radiation. Ionizing radiation induced hyperphosphorylation of Artemis mainly by ATM and in ATM deficient cells to a minor extent by DNA PK. After induction of DSBs with modified ends by a high dose of calicheamicin γ1, Artemis was phosphorylated by DNA PK. The type of calicheamicin γ1‐induced DSBs is likely to represent a subclass of DSBs induced by ionizing radiation. DNA PK‐dependent phosphorylation of Artemis after treatment with DSB inducing agents increased the cellular retention of Artemis, maintained its interaction with DNA ends and activated its endonucleolytic activity. The following model is suggested: ATM‐dependent phosphorylation of Artemis after ionizing radiation could prevent DNA PK‐dependent phosphorylation and activation of undesired endonucleolytic activity at DSBs, which do not require endonucleolytic processing by Artemis. The Artemis:DNA PK complex could be involved in the repair of DSBs, which carry modified ends and are refractory to repair by otherwise lesion specific enzymes because of the presence of an inhibitory lesion in the opposite strand. J. Cell. Biochem. 100: 1346–1351, 2007. © 2007 Wiley‐Liss, Inc.
Fat‐loaded HepG2 spheroids exhibit enhanced protection from Pro‐oxidant and cytokine induced damage
Authors: Damelin Leonard H. , Coward Sam , Kirwan Michael , Collins Peter , Selden Clare , Hodgson Humphrey J. F. 
Published: January 2007

AbstractThe mechanisms by which steatosis renders hepatocytes susceptible to damage in non‐alcoholic steatohepatitis (NASH) are unclear although fat accumulation is believed to increase hepatocyte susceptibility to inflammatory cytokines and oxidative stress. We therefore investigated the susceptibility of steatotic, hepatocyte‐derived cells to TNFα and the pro‐oxidant, t‐butylhydroperoxide (TBH). HepG2 spheroids rendered steatotic by fat‐loading with 0.15 mM oleic or palmitic acid for 48 h and treated with TNFα or TBH for 18 h exhibited surprisingly lower levels of cytotoxicity, and increased anti‐oxidant activity (superoxide dismutase (SOD)) compared with non fat‐loaded controls. The protective effect of steatosis was significantly reversed by the inhibition of AMP‐activated kinase (AMPK) since spheroids transfected with a kinase‐dead AMPKα2 subunit, exhibited a significant increase in TBH‐induced cytotoxicity when fat‐loaded. In conclusion, our findings suggest that fat‐loaded hepatocyte‐derived cells are surprisingly less susceptible to cytokine and pro‐oxidant induced damage via an adaptive mechanism dependent, in part, on AMPK activity. J. Cell. Biochem. 101: 723–734, 2007. © 2007 Wiley‐Liss, Inc.
On the existence of a rainbow 1‐factor in 1‐factorizations of K
Authors: El‐Zanati S.I. , Plantholt M.J. , Sissokho P.A. , Spence L.E. 
Published: January 2007

AbstractLet ${\cal F}$ be a 1‐factorization of the complete uniform hypergraph ${\cal G}={K_{rn}^{(r)}}$ with $r \geq 2$ and $n\geq 3$. We show that there exists a 1‐factor of ${\cal G}$ whose edges belong to n different 1‐factors in ${\cal F}$. Such a 1‐factor is called a “rainbow” 1‐factor or an “orthogonal” 1‐factor. © 2007 Wiley Periodicals, Inc. J Combin Designs 15: 487–490, 2007
Simple 3‐designs of PSL(2, 2n) with block size 7
Authors: Li Weixia , Shen Hao 
Published: January 2007

AbstractIn this paper, we determine the number of the orbits of 7‐subsets of $X= {\rm GF}(2^n)\cup\{\infty\}$ with a fixed orbit length under the action of PSL(2, 2n). As a consequence, we determine the distribution of λ for which there exists a simple 3‐(2n + 1, 7, λ) design with PSL(2, 2n) as an automorphism group. © 2007 Wiley Periodicals, Inc. J Combin Designs 16: 1–17, 2008
Young adults' ideas of cure prior to psychoanalytic psychotherapy
Authors: Philips Björn , Werbart Andrzej , Wennberg Peter , Schubert Johan 
Published: January 2007

AbstractThe study aims to explore systematically the ideas of cure among young adults prior to psychoanalytic psychotherapy. Forty‐six individuals aged 18 to 25 years who applied for psychotherapy underwent the Private Theories Interview (PTI). Twenty distinct categories of ideas of cure were identified. Based on these categories, a theoretical model was constructed with the dimensions, Approaching–Distancing and Doing–Receiving. Individuals were classified into types using “ideal type analysis.” Seven ideal types were formed: Processing and Understanding, Mastering Through Own Will and Action, Talking, Discordant Ideas, Incoherent Ideas, Getting It Out, and Avoiding or Placing the Solution onto Others.
New hypotheses emerged concerning ideas of cure as an important factor for psychotherapy matching, thus potentially predicting premature termination, alliance, and outcome. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 213–232, 2007.
Administration time estimates for Wechsler Intelligence Scale for Children‐IV subtests, composites, and short forms
Authors: Ryan Joseph J. , Glass Laura A. , Brown Cassandra N. 
Published: January 2007

AbstractThe administration times for Wechsler Intelligence Scale for Children‐IV (WISC‐IV) subtests, indexes, and the Full Scale IQ were recorded for 57 school children. Also determined were administration times for eight short forms and the General Ability Index (GAI). All eight short forms reduced testing time by >50%, but the GAI required approximately 56 minutes. The time to administer the 10 core subtests that yield the Full Scale IQ and index scores averaged 72 minutes (range = 42 to 100), but 31% of the administrations required 80 minutes or longer. These results indicate that administration times are quite variable and that D. Wechsler's (2003) guideline of 65 to 80 minutes can be misleading for certain settings and for specific examinees. The present research found administration time to be positively correlated with examinee age, grade placement, and Full Scale IQ. In addition, the extent of examiner experience is known to be positively related to administration speed. In the present research, as in many settings, the examiners were competent, but not highly experienced. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 309–318, 2007.
Seeking help from a mental health professional: The influence of one's social network
Authors: Vogel David L. , Wade Nathaniel G. , Wester Stephen R. , Larson Lisa , Hackler Ashley H. 
Published: January 2007

AbstractThe decision to seek psychological help may be hindered or facilitated by many factors. Two potential factors that might facilitate help seeking are having a relationship with someone (a) who recommends seeking help or (b) who themselves have sought help. In two studies (N= 780,N= 746), we explored the relationship between these factors and intentions to seek mental health services. In Study 1, being prompted to seek help and knowing someone who had sought help were both related to positive expectations about mental health services. In Study 2, being prompted to seek help and knowing someone who had sought help were related to more positive attitudes toward help seeking. Also, knowing someone who had sought help was related to the intention to seek help. Of those who sought psychological help, approximately 75% had someone recommend that they seek help and about 94% knew someone who had sought help. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 233–245, 2007.
Interpersonal profiles in major depressive disorder
Authors: Barrett Marna S. , Barber Jacques P. 
Published: January 2007

AbstractAlthough patients with mood disorders report interpersonal difficulties in addition to depression or anxiety, few studies have examined interpersonal patterns in those patients. Here the authors' goals were to (a) identify the interpersonal pattern in patients with major depressive disorder (MDD), (b) determine interpersonal differences between subgroups of MDD patients, and (c) examine the interpersonal patterns of comorbid MDD patients. One‐ hundred forty‐one MDD adults participated in an ongoing randomized clinical trial of treatments for depression. Interpersonal profiles revealed that MDD patients were significantly more distressed by interpersonal problems than normative samples. Furthermore, MDD patients with depressive personality disorder reported more interpersonal distress than MDD‐only patients report and were more likely to have interpersonal problems related to dominance and control than submissiveness. © 2007 Wiley Periodicals, Inc. J Clin Psychol 63: 247–266, 2007.