bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessing Tidal Models in the Ryukyu Islands Region Using the ENOPF Method
Authors: Zhang Yibo , Kong Chunzheng , Liu Zizhou , Li Bingtian , Lv Xianqing 
Published: February 2024

Abstract
Satellite remote sensing can monitor sea level changes at temporal and spatial scales, plays an important role in the study of tides, and is widely used in numerical tidal models. However, these tidal models are usually computationally expensive. The equidistant nodes orthogonal polynomial fitting (ENOPF) method may overcome that drawback. This study evaluates the accuracy of the ENOPF method in fitting the major tidal constituents in the region near the Ryukyu Islands, where the water depth on either side of the islands varies significantly. The results show that the ENOPF method can accurately fit the major tidal constituents in the presence of complex topography. Furthermore, this approach can also be used to generate reasonable cotidal charts and provide valuable tidal information for hydrodynamic model simulations in the East China Sea. For the high-resolution hydrodynamic model of the East China Sea in particular, reasonable open boundary conditions can be provided by the ENOPF method.
Persistent hiccups as a rare presenting symptom of empyema: a case report
Authors: Lee An-Fu , Lee Hong-Wei , Yen Zui-Shen 
Published: February 2024

Abstract
Background
Empyema is uncommon owing to antibiotic use but still affects patient health if not treated. Hiccups as the initial symptom of empyema are rare; however, empyema should be considered if a patient has persistent hiccups with unexplained fever.

Case presentation
We present a case of persistent hiccups, left upper quadrant abdominal pain, and fever on day 1, and total left lung white-out and empyema on day 3. The hiccups resolved gradually after antibiotic treatment and surgical decortication.

Conclusions
Clinicians should consider the possibility of empyema and conduct a chest computed tomography study if unexplained fever and persistent hiccups coexist.
Acclimation of intertidal macroalgae Ulva prolifera to UVB radiation: the important role of alternative oxidase
Authors: Xu Jinhui , Zhao Xinyu , Zhong Yi , Qu Tongfei , Sun Baixue , Zhang Huanxin , Hou Chengzong , Zhang Zhipeng , Tang Xuexi , Wang Ying 
Published: February 2024

Abstract
Background
Solar radiation is primarily composed of ultraviolet radiation (UVR, 200 − 400 nm) and photosynthetically active radiation (PAR, 400 − 700 nm). Ultraviolet-B (UVB) radiation accounts for only a small proportion of sunlight, and it is the primary cause of plant photodamage. The use of chlorofluorocarbons (CFCs) as refrigerants caused serious ozone depletion in the 1980s, and this had led to an increase in UVB. Although CFC emissions have significantly decreased in recent years, UVB radiation still remains at a high intensity. UVB radiation increase is an important factor that influences plant physiological processes. Ulva prolifera, a type of macroalga found in the intertidal zone, is intermittently exposed to UVB. Alternative oxidase (AOX) plays an important role in plants under stresses. This research examines the changes in AOX activity and the relationships among AOX, photosynthesis, and reactive oxygen species (ROS) homeostasis in U. prolifera under changes in UVB and photosynthetically active radiation (PAR).

Results
UVB was the main component of solar radiation impacting the typical intertidal green macroalgae U. prolifera. AOX was found to be important during the process of photosynthesis optimization of U. prolifera due to a synergistic effect with non-photochemical quenching (NPQ) under UVB radiation. AOX and glycolate oxidase (GO) worked together to achieve NADPH homeostasis to achieve photosynthesis optimization under changes in PAR + UVB. The synergism of AOX with superoxide dismutase (SOD) and catalase (CAT) was important during the process of ROS homeostasis under PAR + UVB.

Conclusions
AOX plays an important role in the process of photosynthesis optimization and ROS homeostasis in U. prolifera under UVB radiation. This study provides further insights into the response of intertidal macroalgae to solar light changes.
Physiological and agronomical traits effects of titanium dioxide nanoparticles in seedlings of Solanum lycopersicum L
Authors: Tighe-Neira Ricardo , Reyes-Díaz Marjorie , Nunes-Nesi Adriano , Lana-Costa Jaciara , Recio Gonzalo , Carmona Erico R. , Acevedo Patricio , Rengel Zed , Inostroza-Blancheteau Claudio 
Published: February 2024

Abstract
Background
Titanium dioxide nanoparticles (TiO2 NPs) have been reported to have contrasting effects on plant physiology, while their effects on sugar, protein, and amino acid metabolism are poorly understood. In this work, we evaluated the effects of TiO2 NPs on physiological and agronomical traits of tomato (Solanum lycopersicum L.) seedlings. Tomato seeds were treated with TiO2 NPs (1000 and 2000 mg L− 1), TiO2 microparticles (µPs, 2000 mg L− 1) as the size control, and ultrapure water as negative control.

Results
The dry matter of stems (DMs), leaves (DMl) and total dry matter (DMt) decreased as particle concentration increased. This trend was also observed in the maximum quantum yield of light-adapted photosystem II (PSII) (Fv´/Fm´), the effective quantum yield of PSII (ΦPSII), and net photosynthesis (Pn). The concentrations of sugars, total soluble proteins, and total free amino acids were unaffected, but there were differences in the daily dynamics of these compounds among the treatments.

Conclusion
Our results suggest that treating tomato seeds with TiO2 might affect PSII performance, net photosynthesis and decrease biomass production, associated with a concentration- and size-related effect of TiO2 particles.
Alternative polyadenylation profiles of susceptible and resistant rice (Oryza sativa L.) in response to bacterial leaf blight using RNA-seq
Authors: Liu Shaochun , Luo Shuqi , Yang Dewei , Huang Junying , Jiang Xinlei , Yu Shangwei , Fu Junru , Zhou Dahu , Chen Xiaorong , He Haohua , Fu Haihui 
Published: February 2024

Abstract
Background
Alternative polyadenylation (APA) is an important pattern of post-transcriptional regulation of genes widely existing in eukaryotes, involving plant physiological and pathological processes. However, there is a dearth of studies investigating the role of APA profile in rice leaf blight.

Results
In this study, we compared the APA profile of leaf blight-susceptible varieties (CT 9737-613P-M) and resistant varieties (NSIC RC154) following bacterial blight infection. Through gene enrichment analysis, we found that the genes of two varieties typically exhibited distal poly(A) (PA) sites that play different roles in two kinds of rice, indicating differential APA regulatory mechanisms. In this process, many disease-resistance genes displayed multiple transcripts via APA. Moreover, we also found five polyadenylation factors of similar expression patterns of rice, highlighting the critical roles of these five factors in rice response to leaf blight about PA locus diversity.

Conclusion
Notably, the present study provides the first dynamic changes of APA in rice in early response to biotic stresses and proposes a possible functional conjecture of APA in plant immune response, which lays the theoretical foundation for in-depth determination of the role of APA events in plant stress response and other life processes.
Characterizing complete mitochondrial genome of Aquilegia amurensis and its evolutionary implications
Authors: Xu Luyuan , Wang Jinghan , Zhang Tengjiao , Xiao Hongxing , Wang Huaying 
Published: February 2024

Abstract
Background
Aquilegia is a model system for studying the evolution of adaptive radiation. However, very few studies have been conducted on the Aquilegia mitochondrial genome. Since mitochondria play a key role in plant adaptation to abiotic stress, analyzing the mitochondrial genome may provide a new perspective for understanding adaptive evolution.

Results
The Aquilegia amurensis mitochondrial genome was characterized by a circular chromosome and two linear chromosomes, with a total length of 538,736 bp; the genes included 33 protein-coding genes, 24 transfer RNA (tRNA) genes and 3 ribosomal RNA (rRNA) genes. We subsequently conducted a phylogenetic analysis based on single nucleotide polymorphisms (SNPs) in the mitochondrial genomes of 18 Aquilegia species, which were roughly divided into two clades: the European-Asian clade and the North American clade. Moreover, the genes mttB and rpl5 were shown to be positively selected in European-Asian species, and they may help European and Asian species adapt to environmental changes.

Conclusions
In this study, we assembled and annotated the first mitochondrial genome of the adaptive evolution model plant Aquilegia. The subsequent analysis provided us with a basis for further molecular studies on Aquilegia mitochondrial genomes and valuable information on adaptive evolution in Aquilegia.
Human biomonitoring without in-person interaction: public health engagements during the COVID-19 pandemic and future implications
Authors: Mattson Alyssa J. , Yu Jiali , Miller Elizabeth M. , Schueller Michael , Pentella Michael , Dai Susie Y. 
Published: February 2024

Abstract
Background
Public health initiatives, including human biomonitoring, have been impacted by unique challenges since the onset of the COVID-19 pandemic, compounding a decades-long trend of declining public participation. To combat low public participation rates, public health professionals often employ extensive engagement approaches including in-person interactions related to enrollment and sampling, success of which is an essential component of a statistically defensible study. The onset of the COVID-19 pandemic challenged public health programs to diversify engagement and sampling approaches, limiting direct interactions for the health and safety of the population. This study explores biomonitoring recruitment strategies through non-contact mechanisms and evaluate the application feasibility for population-based studies.

Methods
The Iowa Biomonitoring Program at the State Hygienic Laboratory developed a human biomonitoring study that utilized a multifaceted, distance-based approach. Traditional techniques, such as mailed recruitment invitations and phone-based discussions, were coupled with internet-based surveys and self-collected, shipped urine and water samples. Participation rates were evaluated by employing different mailing methods, and the demographics of enrolled participants were examined.

Results
This non-human contact approach achieved a nearly 14% participation rate among a rural population, well above our target rates. Our improved mailing strategy for targeting initially unresponsive participants yielded a significantly increase in the participation rates. The respondents were predominantly individuals with educational attainment of at least high school level. Among all the eligible participants, 83% submitted self-collected samples, a rate comparable to the National Health and Nutrition Examination Survey which involved in-person interviews.

Conclusions
The practice of engaging a rural population during the COVID-19 pandemic by transitioning from face-to-face interactions to a combination of mailing and internet-based approaches resulted in higher-than-expected participant recruitment and sample collection rates. Given the declining trend in the response rates for population-based survey studies, our results suggest conducting human biomonitoring without direct human interaction is feasible, which provides further opportunity to improve response rates and the relevance and reach of public health initiatives.
Behavioural determinants shaping infection prevention and control behaviour among healthcare workers in Dutch general practices: a qualitative study reflecting on pre-, during and post-COVID-19 pandemic
Authors: Houben Famke , den Heijer Casper DJ , van Hensbergen Mitch , Dukers-Muijrers Nicole HTM , de Bont Eefje GPM , Hoebe Christian JPA 
Published: February 2024

AbstractBackgroundSince the Coronavirus Disease 2019 (COVID-19) pandemic, awareness of infection prevention and control (IPC) has increased in primary care settings. This study aimed to examine behavioural determinants shaping IPC behaviour pre-, during, and post-pandemic among healthcare workers (HCWs) in general practices, to inform optimised IPC in primary care.MethodsFor this qualitative study, semi-structured in-depth interviews were conducted during two study periods: (1) pre-COVID-19 pandemic: July 2019-February 2020, with 14 general practitioners (GPs) and medical assistants, and (2) during the COVID-19 pandemic: July 2022-February 2023, with 22 GPs and medical assistants. The design was informed by behaviour change theories. Data were analysed using thematic analysis.ResultsMain themes were: (1) risk perception and IPC awareness, (2) attitudes towards IPC and professional responsibility, (3) decision-making process and risk considerations for IPC adherence, (4) social norm and social influence in GP practice team, and (5) environmental context and resource availability in GP practice. During the pandemic, risk perception and awareness of the importance of IPC increased compared to the pre-pandemic period. A consistent belief emerged that IPC is part of professional responsibility, while needing to be balanced with other aspects of patient care. Decision-making is dependent on the individual GP and mainly influenced by risk assessments and sustainability considerations. The social context in the practice team can reinforce IPC behaviours. GP practice building and layout, and limited IPC resource and material availability were reported as main barriers.ConclusionsThe theory-informed insights of this study can be used for targeted interventions to optimise IPC behaviour in general practices. Adopting multifaceted strategies to target the various determinants is recommended to sustain IPC, by implementing continuous education using tailored communication, integrating IPC in work routines and organisational workflows, refining existing IPC protocols by incorporating decision-making tools for HCWs, fostering a culture of IPC through knowledge-sharing and teamwork, and addressing GP practice physical environment and IPC resource barriers.
The Chinese translation and cross-cultural adaptation of PRISMA-7 questionnaire: an observational study to establish the accuracy, reliability and validity
Authors: Yeung Meredith T. , Gan Yen , Teo Shu Qi , Lim Kai Quan , Leow Hui Xuan , Jbabdi Myriam , Raiche Michel , Yang Mingxing 
Published: February 2024

Abstract
Background
Frailty is a health condition linked to adverse health outcomes and lower life quality. The PRISMA-7, a 7-item questionnaire from the Program on Research for Integrating Services for the Maintenance of Autonomy (PRISMA), is a validated case-finding tool for frailty with good sensitivity and specificity. This study aimed to translate, culturally adapt, and validate the PRISMA-7 questionnaire for Chinese use.

Methods
A prospective observational study with convenience sampling recruited bilingual adults aged 65 and over living in the community. The Functional Autonomy Measurement System (SMAF) was the gold standard benchmark. The English PRISMA-7 questionnaire was culturally adapted to Chinese using forward and backward translation. Intra- and inter-rater reliability were determined using the intraclass correlation coefficient (ICC). Face, content and criterion validity were determined. The Receiver Operator characteristic (ROC) curve determined the optimal cut-off score.

Results
One-hundred-twenty participants (55 females and 65 males) were recruited. The Chinese PRISMA-7 questionnaire had excellent intra-rater and inter-rater reliability (ICC = 1.000). The rigorous forward and backward translation established the face and content validity. The moderately high correlations between the English PRISMA-7 with SMAF (r = − 0.655, p <  0.001) and Chinese PRISMA-7 with SMAF (r = − 0.653, p <  0.001) pairs established the criterion validity. An optimal cut-off score of three “Yes” responses was reported with 100% sensitivity and 85.3% specificity.

Conclusion
This translation, cross-cultural adaptation, and validation study established the Chinese PRISMA-7 questionnaire. The preliminary results suggest adequate diagnostic test accuracy for frailty screening among the Chinese-literate community.
Integrated Management of Childhood Illness implementation in Nepal: understanding strategies, context, and outcomes
Authors: Subedi Raj Kumar , VanderZanden Amelia , Adhikari Kriti , Bastola Sasmrita , Hirschhorn Lisa R. , Binagwaho Agnes , Maskey Mahesh 
Published: February 2024

Abstract
Background
Health system-delivered evidence-based interventions (EBIs) are important to reducing amenable under-5 mortality (U5M). Implementation research (IR) can reduce knowledge gaps and decrease lags between new knowledge and its implementation in real world settings. IR can also help understand contextual factors and strategies useful to adapting EBIs and their implementation to local settings. Nepal has been a leader in dropping U5M including through adopting EBIs such as integrated management of childhood illness (IMCI). We use IR to identify strategies used in Nepal’s adaptation and implementation of IMCI.

Methods
We conducted a mixed methods case study using an implementation research framework developed to understand how Nepal outperformed its peers between 2000–2015 in implementing health system-delivered EBIs known to reduce amenable U5M. We combined review of existing literature and data supplemented by 21 key informant interviews with policymakers and implementers, to understand implementation strategies and contextual factors that affected implementation outcomes. We extracted relevant results from the case study and used explanatory mixed methods to understand how and why Nepal had successes and challenges in adapting and implementing one EBI, IMCI.

Results
Strategies chosen and adapted to meet Nepal’s specific context included leveraging local research to inform national decision-makers, pilot testing, partner engagement, and building on and integrating with the existing community health system. These cross-cutting strategies benefited from facilitating factors included community health system and structure, culture of data use, and local research capacity. Geography was a critical barrier and while substantial drops in U5M were seen in both the highest and lowest wealth quintiles, with the wealth equity gap decreasing from 73 to 39 per 1,000 live births from 2001 to 2016, substantial geographic inequities remained.

Conclusions
Nepal’s story shows that implementation strategies that are available across contexts were key to adopting and adapting IMCI and achieving outcomes including acceptability, effectiveness, and reach. The value of choosing strategies that leverage facilitating factors such as investments in community-based and facility-based approaches as well as addressing barriers such as geography are useful lessons for countries working to accelerate adaptation and implementation of strategies to implement EBIs to continue achieving child health targets.
Knowledge, risk perception and uptake of COVID-19 vaccination among internally displaced persons in complex humanitarian emergency setting, Northeast Nigeria
Authors: Gidado Saheed , Musa Melton , Ba’aba Ahmed Ibrahim , Francis Mark Rohit , Okeke Lilian Akudo , Bukar Fatima Lawan , Nguku Patrick M. , Hadejia Idris Suleman , Hassan Isa Ali , Bande Ibrahim Muhammad , Onuoha Martins , Usman Rabi , Ugbenyo Gideon , Godwin Ntadom , Ilori Elsie , Abulfathi Aisha Aliyu , Mshelia Lawi Auta , Mohammed Abede Momoh , Abdullahi Muhammad Maijawa , Bammami Mohammed Isa , Nuorti Pekka , Atkins Salla 
Published: February 2024

Abstract
Background
Owing to crowded and unsanitary conditions, internally displaced persons (IDPs) have an increased risk of COVID-19 infection. Adoption of COVID-19 preventive measures among this population is premised on accurate information, adequate knowledge, and risk perception. We assessed COVID-19 knowledge and risk perception and investigated the association between risk perception and COVID-19 preventive measures, including vaccination among IDPs in Northeast Nigeria.

Methods
We conducted a cross-sectional study during July–December 2022 and sampled 2,175 IDPs using stratified sampling. We utilized a 12-point assessment tool to evaluate COVID-19 knowledge. Participants who scored ≥ 6 points were considered to have adequate knowledge. We used a 30-item Risk Behavior Diagnosis Scale to assess COVID-19 risk perception and evaluated each item on a 5-point Likert scale. Participants were divided into risk perception categories by the median of Likert scale scores. We performed weighted logistic regression analysis to identify factors associated with risk perception. Pearson’s chi-squared with Rao-Scott adjustment was used to determine the relationship between risk perception and COVID-19 preventive measures.

Results
Of 2,175 participants, 55.7% were 18–39 years old, 70.9% were females, and 81.7% had no formal education. Among the IDPs, 32.0% (95% CI: 28.8 – 35.0) were considered to have adequate COVID-19 knowledge, and 51.3% (95% CI: 47.8 – 54.8) perceived COVID-19 risk as high. Moreover, 46.3% (95% CI: 42.8 – 50.0) had received one dose of COVID-19 vaccine, and 33.1% (95% CI: 29.8 – 36.0) received two doses. Adequate knowledge (Adjusted Odds Ratio (AOR) = 2.10, [95% CI: 1.46 – 3.03]) and post-primary education (AOR = 3.20, [95% CI: 1.59 – 6.46]) were associated with risk perception. Furthermore, high risk perception was significantly associated with wearing face masks (χ2 = 106.32, p-value < .001), practicing hand hygiene (χ2 = 162.24, p-value < .001), physical distancing (χ2 = 60.84, p-value < .001) and vaccination uptake (χ2 = 46.85, p-value < .001).

Conclusions
This study revealed gaps in COVID-19 knowledge, risk perception, and vaccination uptake but demonstrated a significant relationship between risk perception and COVID-19 preventive practices. Health education and risk communication should be intensified to improve knowledge, elicit stronger risk perception, and enhance COVID-19 preventive practices.