bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Using Edpuzzle-Based Video in Electrolyte and Nonelectrolyte Solutions Learning on Student Learning Outcomes
Authors: Damanik Dio Ganda Putra , Harta Johnsen 
Published: March 2024

The limitation of using learning media atSMAN 1 Kalasan causes the learning of electrolyte and nonelectrolyte solutions to be less interesting and less effective. Therefore, learning can be supported by attractive video design in the form of Edpuzzle which hasthe potential to build better learning processes and outcomes. This research aims to evaluate the effect of Edpuzzle-based video implementation in electrolyte and nonelectrolyte solutions lessons on student learning outcomes. In form ofa Quasi-Experimental Design, this research was carried out by One-Group Pretest-Posttest Design. The research sample is students of X MIPA 2 that selected by simple random sampling. The instruments used were validation sheets, lesson plans, pretest, and posttest items, questions in Edpuzzle video, an observation sheet of Edpuzzle-based video implementation, and a students’ response questionnaire. Research data were analyzed by SPSS 25, Aiken’s Statistics, and descriptively. The results showed that using Edpuzzle-based video had an influence on students’ learning outcomes that support by Sig value. (2-tailed) < 0.05 andthe average of N-Gain is 0.8 (high). The average post-test is 96.7 (excellent). During Edpuzzle video implementation on learning, the cognitive achievement of C1, C2, and C3respectively levelcompleted are 100%, 100%, and 91.66%, while, C4 and C5 are respectively 22.22% and 41.66%, In addition, the achievement of learning outcomes indicator is also supported by observation for psychomotor and affective domains, with a respectively average scores 96.06% and 95.48%. Edpuzzle-based video can be used as support for electrolyte and nonelectrolyte learning in senior high school.
Development of a Practicum Module Oriented to Chemoentrpreneurship with Qur'an and Hadist Insight on Colloidal System Material
Authors: Roni Roni , Mawarnis Elvy Rahmi , Herman Mimi 
Published: March 2024

This study aims to develop a practicum module oriented to Chemoentrepreneurship with Qur'an and Hadith insight on colloidal system material for Class XI SMA/MA that is valid and practical. This study used the Research and Development (R&D) research method with the 4-D development model (define, design, develop, and disseminate). Data for the defining stage was collected using an instrument in the form of an interview sheet. The interview sheet was filled in by the chemistry teacher. Data for the development stage was collected with instruments in the form of validity sheets and practicality questionnaires. Validation were completed by chemistry lecturers and chemistry teacher. Practicality questionnaire sheets were completed by the students as respondent. Data from validity and practicality instruments were analyzed using descriptive quantitative methods, with the percentage formula.The results showed that the practicum module oriented to Chemoentrepreneurship with Qur'an and Hadith Insight on the material of the colloidal system of class XI SMA / MA was categorized as valid with a percentage of 87.79%. In addition, the practicum module oriented Chemoentrepreneurship with Qur'an and Hadith insight on colloidal system material has met the practical criteria with the results of the student response questionnaire 86.80%. Thus, it is known that the practicum module oriented Chemoentrepreneurship Insightful Qur'an and Hadith on colloidal system material class XI SMA/MA has been valid and practical. The results of this study indicate that the practicum module developed can be used as one of the learning resources for students.
Three-dimensional model of normal human dermal tissue using serial tissue sections
Authors: Liu Peng , Zhang Tao , Huang Yihui 
Published: March 2024

Background: This study aims to construct a three-dimensional model of skin dermis utilizing continuous tissue sections, with the primary objective of obtaining anatomical structure data for normal human dermal tissues.Methods: Normal skin tissue specimens were acquired, paraffin-embedded, and subjected to HE staining. Panoramic images of skin sections were captured using a microscope. Tissue section images were aligned using the SIFT and StackReg image alignment methods, with analysis conducted using the OpenCV module. Mimics17 software facilitated the reconstruction of the skin dermal 3D model, enabling the calculation of dermal porosity and the void diameter.Results: Panoramic skin slices exhibited high-resolution differentiation of dermal fibers and cellular structures. Both SIFT and StackReg image alignment methods yielded similar results, although the SIFT method demonstrated greater robustness. Successful reconstruction of the three-dimensional dermal structure was achieved. Quantitative analysis revealed a dermal porosity of 18.96 ± 4.41% and an average pore diameter of 219.29 ± 34.27 μm. Interestingly, the porosity of the dermis exhibited a gradual increase from the papillary layer to the fourth layer, followed by a transient decrease and then a gradual increase. The distribution of the mean pore diameter mirrored the pattern observed in porosity distribution.Conclusion: Utilizing the continuous skin tissue slice reconstruction technique, this study successfully reconstructed a high-precision three-dimensional tissue structure of the skin. The quantitative analysis of dermal tissue porosity and average pore diameter provides a standardized dataset for the development of biomimetic tissue-engineered skin.
Isoprinosine as a foot-and-mouth disease vaccine adjuvant elicits robust host defense against viral infection through immunomodulation
Authors: Kim Hyeong Won , Ko Mi-Kyeong , Shin Seokwon , Park So Hui , Park Jong-Hyeon , Kim Su-Mi , Lee Min Ja 
Published: March 2024

BackgroundCommercial foot-and-mouth disease (FMD) vaccines have limitations, such as local side effects, periodic vaccinations, and weak host defenses. To overcome these limitations, we developed a novel FMD vaccine by combining an inactivated FMD viral antigen with the small molecule isoprinosine, which served as an adjuvant (immunomodulator).MethodWe evaluated the innate and adaptive immune responses elicited by the novel FMD vaccine involved both in vitro and in vivo using mice and pigs. ResultsWe demonstrated isoprinosine-mediated early, mid-term, and long-term immunity through in vitro and in vivo studies and complete host defense against FMD virus (FMDV) infection through challenge experiments in mice and pigs. We also elucidated that isoprinosine induces innate and adaptive (cellular and humoral) immunity via promoting the expression of immunoregulatory gene such as pattern recognition receptors [PRRs; retinoic acid-inducible gene (RIG)-I and toll like receptor (TLR)9], transcription factors [T-box transcription factor (TBX)21, eomesodermin (EOMES), and nuclear factor kappa B (NF-kB)], cytokines [interleukin (IL)-12p40, IL-23p19, IL-23R, and IL-17A)], and immune cell core receptors [cluster of differentiation (CD)80, CD86, CD28, CD19, CD21, and CD81] in pigs. ConclusionThese findings present an attractive strategy for constructing novel FMD vaccines and other difficult-to-control livestock virus vaccine formulations based on isoprinosine induced immunomodulatory functions.
Improvements in 4th graders' task behavior after physical activity: mediation by inhibition?
Authors: Heemskerk Christina Hubertina Helena Maria , Roebers Claudia M. 
Published: March 2024

IntroductionThis study aimed to investigate if the acute effects of a physical activity (PA) break on the on-task and off-task classroom behavior of primary school children are mediated by inhibition. Combining arousal theory and the cognitive stimulation hypothesis, we employed a 15-min intermittent PA protocol aiming at high-intensity with cognitive demands. We were interested in the effects of PA in real-life settings and in a feasible and sustainable manner for teachers. Thus, the PA session was short and all data collection carried out in ecologically valid school and classroom environments.MethodsFifteen 4th grade classes were randomly assigned to the experimental group (EG; eight classes, n = 120) or waitlist control group (WCG; seven classes, n = 91). Participants were observed during normal classroom lessons for 25 min before and after the PA break (EG) or a business-as-usual lesson (WCG) and completed the Hearts and Flowers task, a task measuring primarily inhibition, once per observation block. We analyzed the effect of PA on inhibition with ANOVA and the effect on behavior and mediation effect with logistic multilevel models.ResultsThe PA break positively affected inhibition with a small effect. Active off-task classroom behavior was higher at post-test in the WCG, but not the EG. Of practical importance, intercept-slope interactions indicated that those with higher levels of off-task behavior at pre-test experienced greater benefits of the PA breaks. No significant mediation of the effect of PA on task-related behaviors via inhibition was found.DiscussionIn conclusion, a time-efficient PA break can improve inhibition and off-task classroom behavior in primary school children. Although these effects occur concurrently, they appear to be independent of each other.
English additional language undergraduate students’ engagement with the academic content in their curriculum in a South African speech-language and hearing training program
Authors: Khoza-Shangase Katijah , Kalenga Margo 
Published: March 2024

BackgroundThe emergence of the Speech-Language and Hearing (SLH) professions in South Africa took place during the era of racial segregation. Consequently, the culture and language of these professions have predominantly reflected the minority White population, even in SLH training programs. English has remained the language of learning and teaching (LOLT) in most South African tertiary institutions, with a strong Western cultural influence. Given the increasing diversity in these institutions, a mismatch between the language of instruction and that of English Additional Language (EAL) students poses academic challenges for this population, prompting the need for this study.AimThis study aims to investigate the learning and social experiences of EAL undergraduate students enrolled in South African SLH training programs, with a specific focus on exploring their engagement with academic content in the curriculum.MethodsA cross-sectional online survey design was employed, and a total of 24 EAL students participated in the study. Descriptive and thematic analysis methods were utilized for data analysis.ResultsThe findings revealed that EAL students encountered academic challenges primarily related to their proficiency in the LOLT, which were most pronounced in lectures and examinations, while being relatively less pronounced in assignments. Complementary use of indigenous languages, simplification of complex terminology and self-employed strategies to cope in response to the existing academic language challenges are highlighted. These findings underscore the significance of addressing pedagogical approaches, language policies, and curriculum transformation towards a more Afrocentric perspective.ConclusionThe study highlights the academic difficulties faced by EAL students in South African SLH training programs. The implications for future interventions in this context are highlighted. The findings emphasize the need for pedagogical reforms, language policy considerations, and curriculum transformation that embraces Afrocentric perspectives. Addressing these issues is crucial to ensure equitable educational experiences and support the success of EAL students in the SLH field.
Adults with excess weight or obesity, but not with overweight, report greater pain intensities than individuals with normal weight: a systematic review and meta-analysis
Authors: Garcia Miguel M. , Corrales Patricia , Huerta Miguel Á. , Czachorowski Maciej J. , López-Miranda Visitación , Medina-Gómez Gema , Cobos Enrique J. , Goicoechea Carlos , Molina-Álvarez Miguel 
Published: March 2024

ContextOver 1.9 billion adult people have overweight or obesity. Considered as a chronic disease itself, obesity is associated with several comorbidities. Chronic pain affects approximately 60 million people and its connection with obesity has been displayed in several studies. However, controversial results showing both lower and higher pain thresholds in subjects with obesity compared to individuals with normal weight and the different parameters used to define such association (e.g., pain severity, frequency or duration) make it hard to draw straight forward conclusions in the matter. The objective of this article is to examine the relationship between overweight and obesity (classified with BMI as recommended by WHO) and self-perceived pain intensity in adults.MethodsA literature search was conducted following PRISMA guidelines using the databases CINAHL, Cochrane Library, EMBASE, PEDro, PubMed, Scopus and Web of Science to identify original studies that provide BMI values and their associated pain intensity assessed by self-report scales. Self-report pain scores were normalized and pooled within meta-analyses. The Cochrane’s Q test and I2 index were used to clarify the amount of heterogeneity; meta-regression was performed to explore the relationship between each outcome and the risk of bias.ResultsOf 2194 studies, 31 eligible studies were identified and appraised, 22 of which provided data for a quantitative analysis. The results herein suggested that adults with excess weight (BMI ≥ 25.0) or obesity (BMI ≥ 30.0) but not with overweight (pre-obesity) alone (BMI 25.0–29.9), are more likely to report greater intensities of pain than individuals of normal weight (BMI 18.5–24.9). Subgroup analyses regarding the pathology of the patients showed no statistically significant differences between groups. Also, influence of age in the effect size, evaluated by meta-regression, was only observed in one of the four analyses. Furthermore, the robustness of the findings was supported by two different sensitivity analyses.ConclusionSubjects with obesity and excess weight, but not overweight, reported greater pain intensities than individuals with normal weight. This finding encourages treatment of obesity as a component of pain management. More research is required to better understand the mechanisms of these differences and the clinical utility of the findings.Systematic Review Registrationhttps://doi.org/10.17605/OSF.IO/RF2G3, identifier OSF.IO/RF2G3.
Diluted bitumen weathered under warm or cold temperatures is equally toxic to freshwater fish
Authors: Gutierrez-Villagomez Juan Manuel , Lara-Jacobo Linda Ramona , Gauthier Charles , Patey Geraldine , Xin Qin , Triffault-Bouchet Gaëlle , Dettman Heather D. , Langlois Valerie S. 
Published: March 2024

Canada is one of the main petroleum producers in the world. Through its oil sands exploitation, a viscous bitumen mixed with sand, water, and clay is being produced. This bitumen is so viscous that approximatively 20%–30% of diluent needs to be added to ease transportation, resulting in a mixture called diluted bitumen (dilbit). The transport of dilbit through North America comes with a potential risk for oil spills in freshwater ecosystems at any time of the year. In this study, a mesoscale spill tank was used to study dilbit spills in freshwater to understand the effect of cold (winter-like) vs. warmer (spring- and fall-like) water temperatures on its natural weathering and their toxicity to fathead minnow (Pimephales promelas) embryos. Water samples were collected weekly during two consecutive 35-day experiments ran at either 2 or 15 °C. Each week, fish larvae were exposed for 7 days, and water analysis was performed. Chemical analysis showed that the volatile organic compound, total organic carbon, and polycyclic aromatic hydrocarbon concentrations decreased in both experiments with time, while fish larvae exposed to both temperature settings yielded increased abnormalities, EROD activity, CYP1A, and glutathione S-transferase mRNA expression levels, and decreased heart rate. Importantly, there were no major differences between the temperature regimes on dilbit weathering, highlighting that if a spill occurs in colder waters, it would be equally toxic to organisms. This work provides new data on the potential risk of oil spill for use during response planning and modelling.
Watershed analysis of urban stormwater contaminant 6PPD-Quinone hotspots and stream concentrations using a process-based ecohydrological model
Authors: Halama Jonathan J. , McKane Robert B. , Barnhart Bradley L. , Pettus Paul P. , Brookes Allen F. , Adams Angela K. , Gockel Catherine K. , Djang Kevin S. , Phan Vivian , Chokshi Sonali M. , Graham James J. , Tian Zhenyu , Peter Katherine T. , Kolodziej Edward P. 
Published: March 2024

Coho salmon (Oncorhynchus kisutch) are highly sensitive to 6PPD-Quinone (6PPD-Q). Details of the hydrological and biogeochemical processes controlling spatial and temporal dynamics of 6PPD-Q fate and transport from points of deposition to receiving waters (e.g., streams, estuaries) are poorly understood. To understand the fate and transport of 6PPD and mechanisms leading to salmon mortality Visualizing Ecosystem Land Management Assessments (VELMA), an ecohydrological model developed by US Environmental Protection Agency (EPA), was enhanced to better understand and inform stormwater management planning by municipal, state, and federal partners seeking to reduce stormwater contaminant loads in urban streams draining to the Puget Sound National Estuary. This work focuses on the 5.5 km2 Longfellow Creek upper watershed (Seattle, Washington, United States), which has long exhibited high rates of acute urban runoff mortality syndrome in coho salmon. We present VELMA model results to elucidate these processes for the Longfellow Creek watershed across multiple scales–from 5-m grid cells to the entire watershed. Our results highlight hydrological and biogeochemical controls on 6PPD-Q flow paths, and hotspots within the watershed and its stormwater infrastructure, that ultimately impact contaminant transport to Longfellow Creek and Puget Sound. Simulated daily average 6PPD-Q and available observed 6PPD-Q peak in-stream grab sample concentrations (ng/L) corresponds within plus or minus 10 ng/L. Most importantly, VELMA’s high-resolution spatial and temporal analysis of 6PPD-Q hotspots provides a tool for prioritizing the locations, amounts, and types of green infrastructure that can most effectively reduce 6PPD-Q stream concentrations to levels protective of coho salmon and other aquatic species.
Unraveling the intricacies of glioblastoma progression and recurrence: insights into the role of NFYB and oxidative phosphorylation at the single-cell level
Authors: Liu Pulin , Xing Naifei , Xiahou Zhikai , Yan Jingwei , Lin Zhiheng , Zhang Junlong 
Published: March 2024

BackgroundGlioblastoma (GBM), with its high recurrence and mortality rates, makes it the deadliest neurological malignancy. Oxidative phosphorylation is a highly active cellular pathway in GBM, and NFYB is a tumor-associated transcription factor. Both are related to mitochondrial function, but studies on their relationship with GBM at the single-cell level are still scarce.MethodsWe re-analyzed the single-cell profiles of GBM from patients with different subtypes by single-cell transcriptomic analysis and further subdivided the large population of Glioma cells into different subpopulations, explored the interrelationships and active pathways among cell stages and clinical subtypes of the populations, and investigated the relationship between the transcription factor NFYB of the key subpopulations and GBM, searching for the prognostic genes of GBM related to NFYB, and verified by experiments.ResultsGlioma cells and their C5 subpopulation had the highest percentage of G2M staging and rGBM, which we hypothesized might be related to the higher dividing and proliferating ability of both Glioma and C5 subpopulations. Oxidative phosphorylation pathway activity is elevated in both the Glioma and C5 subgroup, and NFYB is a key transcription factor for the C5 subgroup, suggesting its possible involvement in GBM proliferation and recurrence, and its close association with mitochondrial function. We also identified 13 prognostic genes associated with NFYB, of which MEM60 may cause GBM patients to have a poor prognosis by promoting GBM proliferation and drug resistance. Knockdown of the NFYB was found to contribute to the inhibition of proliferation, invasion, and migration of GBM cells.ConclusionThese findings help to elucidate the key mechanisms of mitochondrial function in GBM progression and recurrence, and to establish a new prognostic model and therapeutic target based on NFYB.
Identification of hub genes within the CCL18 signaling pathway in hepatocellular carcinoma through bioinformatics analysis
Authors: Mao Jinlei , Tao Yuhang , Wang Keke , Sun Hanru , Zhang Manqi , Jin Liang , Pan Yi 
Published: March 2024

IntroductionHepatocellular carcinoma (HCC) is an aggressive malignancy, and CCL18, a marker of M2 macrophage activation, is often associated with tumor immune suppression. However, the role of CCL18 and its signaling pathway in HCC is still limited. Our study focuses on investigating the prognostic impact of CCL18 and its signaling pathway in HCC patients and biological functions in vitro.MethodsHCC-related RNA-seq data were obtained from TCGA, ICGC, and GEO. The 6 hub genes with the highest correlation to prognosis were identified using univariate Cox and LASSO regression analysis. Multivariate Cox regression analysis was performed to assess their independent prognostic potential and a nomogram was constructed. In vitro experiments, including CCK8, EdU, RT-qPCR, western blot, and transwell assays, were conducted to investigate the biological effects of exogenous CCL18 and 6 hub genes. A core network of highly expressed proteins in the high-risk group of tumors was constructed. Immune cell infiltration was evaluated using the ESTIMATE and CIBERSORT packages. Finally, potential treatments were explored using the OncoPredict package and CAMP database.ResultsWe identified 6 survival-related genes (BMI1, CCR3, CDC25C, CFL1, LDHA, RAC1) within the CCL18 signaling pathway in HCC patients. A nomogram was constructed using the TCGA_LIHC cohort to predict patient survival probability. Exogenous CCL18, as well as overexpression of BMI1, CCR3, CDC25C, CFL1, LDHA, and RAC1, can promote proliferation, migration, invasion, stemness, and increased expression of PD-L1 protein in LM3 and MHCC-97H cell lines. In the high-risk group of patients from the TCGA_LIHC cohort, immune suppression was observed, with a strong correlation to 21 immune-related genes and suppressive immune cells.ConclusionExogenous CCL18 promotes LM3 and MHCC-97H cells proliferation, migration, invasion, stemness, and immune evasion. The high expression of BMI1, CCR3, CDC25C, CFL1, LDHA, and RAC1 can serve as a biomarkers for immune evasion in HCC.