bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between vitamin D3 deficiency and insulin resistance in pregnancy
Authors: Maghbooli Zhila , Hossein‐nezhad Arash , Karimi Farzaneh , Shafaei Ali‐Reza , Larijani Bagher 
Published: July 2007

AbstractBackgroundThe serum level of 25‐hydroxyvitamin D deficiency has long been suspected as a risk factor for glucose intolerance and perhaps 1,25‐dihydroxyvitamin D has a role in the regulation of insulin secretion. This study investigates the relation between 25‐hydroxyvitamin D concentrations and insulin resistance in pregnant women.MethodsA cross‐sectional study was conducted on 741 pregnant women referred to five educating hospital clinics. Universal screening was performed with a GCT‐50 g, and those with plasma glucose levels ≥ 7.2 mmol/L were diagnosed as GDM if they had an impaired GTT‐100 g based on Carpenter and Coustan criteria. The levels of insulin and C‐peptide were measured during OGTT‐100 g test. The homeostasis model assessment index (HOMA) equation was used as the insulin resistance index. The concentrations of 25‐hydroxyvitamin D, and PTH were also measured.ResultsTotal prevalence of vitamin D deficiency (<25 nmol/L) was found in 70.6% of pregnant women. Prevalence of severe vitamin D deficiency (<12.5) in GDM patients was higher than in normoglycaemic pregnancies. The regression model revealed a strong correlation between the HOMA index and serum levels of vitamin D.ConclusionsThese results show that a positive correlation of 25(OH) vitamin D concentrations with insulin sensitivity and vitamin D deficiency could be a confirmative sign of insulin resistance. Copyright © 2007 John Wiley & Sons, Ltd.
Lentivirus as a tool for lineage‐specific gene manipulations
Authors: Malashicheva Anna , Kanzler Benoît , Tolkunova Elena , Trono Didier , Tomilin Alexey 
Published: July 2007

AbstractThe trophectoderm (TE) of blastocysts, the first epithelium established in mammalian development, (1) plays signaling, supportive, and patterning functions during preimplantation development, (2) ensures embryo implantation into the uterine wall, and (3) gives rise to extraembryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive to lentiviral (LV) infection, represents a robust nonpermeable physical barrier to the virus particles, thereby shielding the cells of the inner cell mass from viral infection. This LV feature will allow modulations of gene expression in a lineage‐specific manner, thus having significant applications in mouse functional genetics. genesis 45:456–459, 2007. © 2007 Wiley‐Liss, Inc.
Factors associated with suicidal behaviors in a large French sample of inpatients with eating disorders
Authors: Fedorowicz Valérie J. , Falissard Bruno , Foulon Christine , Dardennes Roland , Divac Snezana M. , Guelfi Julien D. , Rouillon Frédéric 
Published: July 2007

AbstractObjective:The objective of the present study was to identify factors associated with suicidal behaviors among patients with eating disorders.Method:A large database including sociodemographic and clinical characteristics of 1,009 consecutive patients hospitalized for an eating disorder in Paris, France, was examined. Data gathered upon admission to hospital were analyzed to identify factors associated with a history of suicide attempt or current suicidal ideation, among the whole sample as well as among each subtype of eating disorder.Results:Among the whole sample, the factor most strongly associated with suicide attempt or suicidal ideation was the diagnostic category, with the highest odds ratio for bulimia nervosa followed by anorexia nervosa of the binging/purging subtype. Among diagnostic subgroups, the strongest factors were drug use, alcohol use, and tobacco use.Conclusion:Suicide risk should be monitored carefully among patients with eating disorders, paying particular attention to combinations of risk factors. © 2007 by Wiley Periodicals, Inc. Int J Eat Disord 2007
Cue exposure in the treatment of resistant adolescent bulimia nervosa
Authors: Martinez‐Mallén Esteban , Castro‐Fornieles Josefina , Lázaro Luisa , Moreno Elena , Morer Astrid , Font Elena , Julien Joana , Vila Montserrat , Toro Josep 
Published: July 2007

AbstractObjective:A percentage of bulimic patients do not greatly improve with the usual treatment. Therefore, the objective was to further evaluate cue exposure (CE), in order to attain better results in clinical settings.Method:Twenty‐two adolescent patients who fulfilled DSM‐IV diagnostic criteria for bulimia nervosa (mean age 16.7, SD 1.5) and who were resistant to the usual treatment followed a program of 12 CE sessions. Clinical characteristics were evaluated and different psychopathological scales were administered at the beginning and the end of the CE program and at 6 month follow‐up. Subjective anxiety and physiological parameters were recorded during the sessions.Results:A significant decrease was observed in subjective anxiety (p = .023), heart rate (p < .001), and blood pressure (p = .001) during the first session. A decrease in these parameters was also recorded between the first and the last session. The number of binges per week (p = .005) and the mean score for the psychopathological scales decreased significantly from the beginning of the treatment, and were significantly lower at the end of the CE program and at follow‐up. Purging behaviors per week only decreased significantly after the end of the CE session during the follow‐up (p = .04).Conclusion:Anxiety, bingeing, purging, and psychopathological scales improve with a CE program in resistant bulimia nervosa. © 2007 by Wiley Periodicals, Inc. Int J Eat Disord 2007
Chromosome instability and translocation t(11;18) in primary gastric marginal zone B‐cell lymphoma of MALT‐type
Authors: Tibiletti Maria Grazia , Milani Katia , Martin Vittoria , Zucca Emanuele , Motta Teresio , Cortelazzo Sergio , Pinotti Graziella , Mazzucchelli Luca , Pruneri Giancarlo , Martinelli Giovanni , Barbazza Renzo , Capella Carlo , Bertoni Francesco 
Published: July 2007

AbstractThe prognosis for patients with mucosa‐associated lymphoid tissue (MALT) lymphomas is good; these tumours have usually an indolent course with overall survival rates that are greater than 80% at 5‐year, but some rare cases with histological transformation in aggressive diffuse large cell lymphoma have also been diagnosed. Here, we present cytogenetic results on endoscopic bioptic material of 42 cases of primary gastric extranodal marginal zone B‐cell lymphoma (EMZL) using fluorescence in situ hybridization (FISH) approach with API2, MALT1 and centromeric probes for chromosome 3 and 18, and their impact on the clinical outcome. Copyright © 2007 John Wiley & Sons, Ltd.
Surface flux and atmospheric boundary layer observations from the LAPS project over the middle stream of the Huaihe River basin in China
Authors: Tanaka Hiroki , Hiyama Tetsuya , Yamamoto Koh , Fujinami Hatsuki , Shinoda Taro , Higuchi Atsushi , Endo Satoshi , Ikeda Shoichiro , Li Weijing , Nakamura Kenji 
Published: July 2007

AbstractObservations of the surface layer and the atmospheric boundary layer (ABL) were collected as part of the Lower Atmosphere and Precipitation Study (LAPS), which investigated the relationship between the surface conditions and the ABL processes. The LAPS was part of the Core Research for Evolutional Science and Technology (CREST) program, under the auspices of the Japan Science and Technology Agency (JST). Observations began in August 2003 over a flat surface region in mid‐latitude China at 32·55°N, 116·78°E. Observations before, during, and after the Meiyu season in China provided data for surface conditions varying from relatively dry to moist. Preliminary analysis of the surface and the ABL observations shows relationships between the surface fluxes and the ABL structure. ABL depth was enhanced by sensible heat flux. Fluctuations in the ABL depth corresponded to plume‐like wind structures within the ABL. Day‐to‐day variability in ABL depth was controlled mainly by buoyancy flux over the surface during dry periods. It was also affected by vertical motion at the top of the ABL, especially during wet periods. Copyright © 2007 John Wiley & Sons, Ltd.
Strain field measurements on mouse carotid arteries using microscopic three‐dimensional digital image correlation
Authors: Sutton M.A. , Ke X. , Lessner S.M. , Goldbach M. , Yost M. , Zhao F. , Schreier H.W. 
Published: July 2007

AbstractA stereomicroscope system is adapted to make accurate, quantitative displacement, and strain field measurements with microscale spatial resolution and nanoscale displacement resolution on mouse carotid arteries. To perform accurate and reliable calibration for these systems, a two‐step calibration process is proposed and demonstrated using a modification to recently published procedures. Experimental results demonstrate that the microscope system with three‐dimensional digital image correlation (3D‐DIC) successfully measures the full 3D displacement and surface strain fields at the microscale during pressure cycling of 0.40‐mm‐diameter mouse arteries, confirming that the technique can be used to quantify changes in local biomechanical response which may result from variations in extracellular matrix composition, with the goal of quantifying properties of the vessel. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Release rate controls biological activity of nerve growth factor released from fibrin matrices containing affinity‐based delivery systems
Authors: Wood Matthew D. , Sakiyama‐Elbert Shelly E. 
Published: July 2007

AbstractPreviously, combinatorial techniques were used to identify peptide sequences exhibiting high, medium, and low affinity for heparin. Bidomain peptides were synthesized containing a transglutaminase sequence for one domain and one of the heparin‐affinity sequences for the other domain. A delivery system was made consisting of bi‐domain peptides, heparin, and nerve growth factor (NGF), which binds to heparin with moderate affinity. The goal of this research was to determine whether peptide affinity for heparin and the molar ratio of peptide to heparin affected the release rate of NGF from the delivery system and the biological activity of NGF released. This study also explored whether peptide affinity modulated biological activity independent of release rate. Mathematically modeling the delivery system confirmed that release could be controlled by both peptide affinity and molar ratio of peptide to heparin. Experimentally, the rate of NGF release from the delivery system was found to be affected by peptide affinity and molar ratio. The delivery system presented biologically active NGF as assayed by embryonic chick dorsal root ganglia (DRGs) neurite extension, where extension was similar to or increased for DRGs grown in fibrin matrices containing the delivery system compared to DRGs grown with NGF in the culture media. Furthermore, by modulating the molar ratio of peptide to heparin in the delivery system, similar release rates of NGF were obtained for different affinity peptides and these conditions promoted similar levels of neurite extension, demonstrating that release rate appears to be the main mechanism controlling the biological activity of released NGF. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Bone tissue engineering using human adipose‐derived stem cells and honeycomb collagen scaffold
Authors: Kakudo Natsuko , Shimotsuma Ayuko , Miyake Shogo , Kushida Satoshi , Kusumoto Kenji 
Published: July 2007

AbstractHuman adipose‐derived stem cells (ASCs) have the capacity to regenerate and the potential to differentiate into multiple lineages of mesenchymal cells. The aim of this study was to investigate the possibility of using honeycomb collagen scaffold to culture ASCs in bone tissue engineering. The osteogenic capacity of ASCs in vitro, was confirmed by histology and measuring the expression of cbfa‐1. After that, ASCs were cultured for up to 14 days in the honeycomb scaffold to allow a high density, three‐dimensional culture. Scanning electron microscopy data showed that the scaffold was filled with the grown ASCs, and calcification, stained black with von Kossa, was confirmed. Furthermore, The ASC‐loaded honeycomb collagen scaffolds cultured for 14 days were subcutaneously transplanted into nude mice, and excised after 8 weeks. Bone formation in vivo was examined using HE stain, von Kossa stain, and osteocalcin immunostain. Those histological views showed significant positive stains in the samples of osteogenic medium in the three types of stain. These results suggest that this carrier is a suitable scaffold for ASCs and will be useful as a three‐dimensional bone tissue engineering scaffold in vitro and in vivo. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Metacarpophalangeal collateral ligament reconstruction using small intestinal submucosa in an equine model
Authors: Bertone Alicia L. , Goin Scott , Kamei Sachiko J. , Mattoon John S. , Litsky Alan S. , Weisbrode Steven E. , Clarke Rhonda B. , Plouhar Pamela L. , Kaeding Christopher C. 
Published: July 2007

AbstractXenogeneic porcine small intestinal submucosa (SIS) is a natural, biodegradable matrix that has been successfully used as a scaffold for repair of tissue defects. The goal of this study was to compare a collateral ligament transection surgically reconstructed with an anchored SIS ligament to a sham‐operated control procedure for the correction of joint laxity using an equine model. Ten metacarpophalangeal joints from 10 horses had complete transection of the lateral collateral ligament. In 6 horses, the collateral ligament was reconstructed with a multilaminate strip of SIS anchored with screws into bone tunnels proximal and distal to the joint. The sham controls had similar screws, but no SIS placed. Clinical compatibility and effectiveness were evaluated with lameness, incisional quality, and joint range of motion, circumference and laxity. Ligament structure and strength was quantified with serial high resolution ultrasound, histology, and mechanical testing at 8 weeks. Surgical repair with SIS eliminated joint laxity at surgery. SIS‐treated joints had significantly less laxity than sham treatment at 8 weeks (p< 0.001). SIS‐treated ligaments demonstrated a progressive increase in repair tissue density and fiber alignment that by week 8 were significantly greater than sham‐treated ligament (p< 0.03). SIS‐repaired ligament tended to have greater peak stress to failure than sham‐treatment (p< 0.07). Cellularity within the ligament repair tissue and inflammation within the bone tunnel was significantly greater in the SIS‐treated limbs (p< 0.017). Within the first 8 weeks of healing, SIS implanted to reinforce collateral ligament injury was biocompatible in the joint environment, restored initial loss of joint stability, and accelerated early repair tissue quality. SIS ligament reconstruction might provide benefit to early ligament healing and assist early joint stability associated with ligament injury. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Homogeneous chitosan‐PLGA composite fibrous scaffolds for tissue regeneration
Authors: Shim In Kyung , Lee Sang Young , Park Yoon Jeong , Lee Myung Chul , Lee Sang Hoon , Lee Jue Yeon , Lee Seung Jin 
Published: July 2007

AbstractNovel chitosan‐poly(lactide‐co‐glycolide) (PLGA) composite fibers and nonwoven fibrous scaffolding matrices were designed for cartilage regeneration. A homogenous one‐phase mixture of chitosan and PLGA at a ratio of 50:50 (w/w %) was successfully produced using cosolvents of 1,1,1,3,3,3‐hexafluoroisopropanol and methylene chloride. A wet spinning technique was employed to fabricate composite fibrous matrices. Physical characterizations of one‐phase chitosan‐PLGA composite (C/Pc) matrices were performed for their homogeneity, in vitro degradability, mechanical property and wettability in comparison to two‐phase chitosan and PLGA composite fibrous matrices in which PLGA was dispersed in a continuous chitosan phase. The one‐phase property of C/Pc matrices was confirmed from thermal analysis. Significantly retarded degradation was observed from the composite C/Pc fibrous matrices in contrast to the PLGA‐dispersed chitosan (C/Pd) fibrous matrices due to the effective acid‐neutralizing effect of chitosan on acid metabolites of PLGA. The composition of chitosan with PLGA resulted in a characteristic soft and strong mechanical property that could not be retained by either PLGA or the chitosan fibers. In addition, the presence of chitosan in the composite matrices provided proper wettability for cell cultivation. The C/Pc matrices were further investigated for their scaffolding function using chondrocytes for cartilage regeneration. Enhanced cell attachment was observed on the composite matrix compared with the PLGA fibrous matrices. The mRNA expression of type II collagen and aggrecan was upregulated in the composite matrix owing to the superior cell compatibility of chitosan. These results suggest an excellent potential for C/Pc one‐phase composite fibrous matrices as scaffolding materials for tissue regeneration. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Controlled release of an antibiotic, gentamicin sulphate, from gravity spun polycaprolactone fibers
Authors: Chang H.‐I. , Lau Y.‐C. , Yan C. , Coombes A.G.A. 
Published: July 2007

AbstractThe antibiotic, gentamicin sulphate (GS), was incorporated in gravity‐spun polycaprolactone (PCL) fibers by spinning from particulate suspensions of the drug in PCL solution to produce a controlled delivery system. The production rate of GS‐loaded PCL fibers was confined to the range 1–1.5 m/min and the fiber diameter to 170–220 μm. The kinetics of drug release could be adjusted by varying the GS loading of the fibers and the suspension preparation conditions. Gradual release of ∼80% of the initial GS content was measured in phosphate buffered saline at 37°C over 50 days from fibers spun from nonhomogenized suspensions, whereas loss of this amount of antibiotic occurred in less than 10 days from fibers spun from homogenized suspensions. Studies of growth inhibition of Stapyhlococcus epidermidis in culture indicated that GS released after 2 weeks from PCL fibers retained antibacterial activity. This behavior recommends further investigation of PCL fibers for local delivery of antibiotics to combat infection associated with periodontal disease, musculoskeletal injuries, and implantation of fiber‐based tissue substitutes such as vascular prostheses. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008
Bond length and the electron density at the bond critical point: XX, ZZ, and CZ bonds (X = Li‐F, Z = Na‐Cl)
Authors: Castillo Norberto , Robertson Katherine N. , Choi S. C. , Boyd Russell J. , Knop Osvald 
Published: July 2007

AbstractThe aim was to investigate the relationship between the bond length and the electron density at the bond critical point in homonuclear XX and ZZ and heteronuclear CZ bonds (X = Li‐F, Z = Na‐Cl). Thed,ρcpairs were obtained from 472 target bonds in DFT‐optimized (B3LYP/6‐311+G(d,p)) small molecular species. These species were selected arbitrarily but with a view to maximize the range widthsWRfor each atom combination. It was found that (i) with one clear exception, thed(A−A) means (A = X or Z) correlate linearly with the bond lengthsd(A2) of the respective diatomic molecules; (ii) thed(A−A) means correlate parabolically withn, the formal number of valence electrons in the atoms of the bond; and (iii) with increasing sample size N the ratioWR(ρc)/WR(d) appears to converge toward a representationf[WR(ρc)/WR(d)]N→∞characteristic of A. Detailed analysis of thed,ρcrelationship has shown that by and large simple power regression accounts best for the DFT data. The regression coefficients ofd=aρand ρc= αd−β(b,β> 0) vary withnin a seemingly irregular manner but one that is consistent with simple chemical notions. Thed(A2) can be approximated in terms of multilinear MO electron occupancies. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2008
Zebrafish dentition in comparative context
Authors: Stock David W. 
Published: July 2007

AbstractStudies of the zebrafish (Danio rerio) promise to contribute much to an understanding of the developmental genetic mechanisms underlying diversification of the vertebrate dentition. Tooth development, structure, and replacement in the zebrafish largely reflect the primitive condition of jawed vertebrates, providing a basis for comparison with features of the more extensively studied mammalian dentition. A distinctive derived feature of the zebrafish dentition is restriction of teeth to a single pair of pharyngeal bones. Such reduction of the dentition, characteristic of the order Cypriniformes, has never been reversed, despite subsequent and extensive diversification of the group in numbers of species and variety of feeding modes. Studies of the developmental genetic mechanism of dentition reduction in the zebrafish suggest a potential explanation for irreversibility in that tooth loss seems to be associated with loss of developmental activators rather than gain of repressors. The zebrafish and other members of the family Cyprinidae exhibit species‐specific numbers and arrangements of pharyngeal teeth, and extensive variation in tooth shape also occurs within the family. Mutant screens and experimental alteration of gene expression in the zebrafish are likely to yield variant tooth number and shape phenotypes that can be compared with those occurring naturally within the Cyprinidae. Such studies may reveal the relative contribution to trends in dental evolution of biases in the generation of variation and sorting of this variation by selection or drift.J. Exp. Zool. (Mol. Dev. Evol.) 308B:523–549, 2007. © 2007 Wiley‐Liss, Inc.
Oxidation of 14C‐labeled compounds perfused by microdialysis in the brains of free‐moving rats
Authors: Zielke H. Ronald , Zielke Carol L. , Baab Peter J. 
Published: July 2007

AbstractThe oxidative capacity of the brain for alternate substrates, glucose, lactate, pyruvate, acetate, glutamate, and glutamine was determined by using microdialysis to infuse 14C‐labeled compounds into the interstitial fluid of adult rat brain and by collecting the brain‐generated 14CO2 from the dialysis eluate. All compounds were readily oxidized. The recovery of 14CO2 was enhanced for those compounds metabolically close to entry into the TCA cycle or known to have a low interstitial concentration. Two compounds, pyruvate and lactate, demonstrated reciprocal competition when added as nonradioactive competitors. Oxidation of two amino acids, 14C‐glutamate and 14C‐glutamine, was stimulated by the addition of nonradioactive acetate and pyruvate. α‐Cyano‐4‐hydroxycinnamate decreased 14C‐lactate and 14C‐pyruvate oxidation, consistent with the transport of both compounds via a monocarboxylate transporter. The results of this in vivo study support the results of previous in vitro studies that showed that a wide range of compounds formed from glucose in the brain are also oxidized in the brain for energy production. © 2007 Wiley‐Liss, Inc.
Sensitivity enhancement in saturation transfer difference (STD) experiments through optimized excitation schemes
Authors: Cutting Brian , Shelke Sachin V. , Dragic Zorica , Wagner Beatrice , Gathje Heiko , Kelm Soerge , Ernst Beat 
Published: July 2007

AbstractInvestigation of ligand–protein interactions by the saturation transfer difference (STD) experiment has been well established in the drug discovery process through numerous examples. Thus, binding epitopes may be mapped by comparing signals of the ligand with and without saturation of the protein. Herein, it is shown that a less selective process allows more protons to assist in the saturation of the protein, thereby considerably enhancing the sensitivity of the STD experiment. Increasing the saturation power entails a greater risk of perturbing the ligand; however, an amplitude modulation of the waveform assists this procedure by distributing the applied energy in sidebands. Copyright © 2007 John Wiley & Sons, Ltd.