bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vegetative, EEG and Nystagmic Reactivity to Vestibular Stimulation in Patients with Cerebral Vascular Disorders
Authors: Poilici I. , Crighel E. 
Published: May 2007

Nystagmic, EEG and vegetative reactions i. e. digital plethysmogram, electrodermogram, rheoencephalogram, were investigated in 15 controls and in 42 patients with cerebro-vascular diseases.	The frequency of autonomic, EEG and nystagmic reactions to vestibular stimuli was significantly different in patients with cerebro-vascular diseases from	controls. That pointed to differentiated reactions depending on the type of lesion and on the site of connection between the vestibular afferents and oculomotor and autonomic efferents. It was also possible to distinguish the nonspecific orienting reflex, appearing at the beginning of ear irrigation and the vestibular reaction, appearing late, after the end of irrigation.
Neural Dynamics of Rejection Sensitivity
Authors: Kross Ethan , Egner Tobias , Ochsner Kevin , Hirsch Joy , Downey Geraldine 
Published: May 2007

Abstract
Rejection sensitivity (RS) is the tendency to anxiously expect, readily perceive, and intensely react to rejection. This study used functional magnetic resonance imaging to explore whether individual differences in RS are mediated by differential recruitment of brain regions involved in emotional appraisal and/or cognitive control. High and low RS participants were scanned while viewing either representational paintings depicting themes of rejection and acceptance or nonrepresentational control paintings matched for positive or negative valence, arousal and interest level. Across all participants, rejection versus acceptance images activated regions of the brain involved in processing affective stimuli (posterior cingulate, insula), and cognitive control (dorsal anterior cingulate cortex; medial frontal cortex). Low and high RS individuals' responses to rejection versus acceptance images were not, however, identical. Low RS individuals displayed significantly more activity in left inferior and right dorsal frontal regions, and activity in these areas correlated negatively with participants' self-report distress ratings. In addition, control analyses revealed no effect of viewing negative versus positive images in any of the areas described above, suggesting that the aforementioned activations were involved in rejection-relevant processing rather than processing negatively valenced stimuli per se. Taken together, these findings suggest that responses in regions traditionally implicated in emotional processing and cognitive control are sensitive to rejection stimuli irrespective of RS, but that low RS individuals may activate prefrontal structures to regulate distress associated with viewing such images.
Grammar or Serial Order?: Discrete Combinatorial Brain Mechanisms Reflected by the Syntactic Mismatch Negativity
Authors: Pulvermüller Friedemann , Assadollahi Ramin 
Published: May 2007

Abstract
If word strings violate grammatical rules, they elicit neurophysiological brain responses commonly attributed to a specifically human language processor or grammar module. However, an ungrammatical string of words is always also a very rare sequence of events and it is, therefore, not always evident whether specifically linguistic processes are at work when neurophysiological grammar indexes are being reported. We here investigate the magnetic mismatch negativity (MNN) to ungrammatical word strings, to very rare grammatical strings, and to common grammatical phrases. In this design, serial order mechanism mapping the sequential probability of words should neurophysiologically dissociate frequent grammatical phrases from both ungrammatical and rare grammatical strings. However, if syntax as a discrete combinatorial system is reflected, the prediction is that the rare, correctly combined items group with the highly frequent grammatical strings and stand out against ungrammatical strings. Using magnetoencephalography as a measure of human brain activity, we replicated the previously reported syntactic mismatch negativity (sMMN), which distinguishes highly unfamiliar ungrammatical word sequences from common grammatical strings. Crucially, a significant interaction demonstrated that the sMMN specifically distinguished syntactic violations from common grammatical strings, but not uncommon from common grammatical word strings. This significant interaction argues in favor of a genuinely grammatical origin of the sMMN and provides direct neurophysiological evidence for a discrete combinatorial system for word and morpheme sequences in the human brain. The data are more difficult to explain in the context of serial order models that map co-occurrence probabilities of words.
Variability of North Pacific Sea Ice and East Asia–North Pacific Winter Climate
Authors: Liu Jiping , Zhang Zhanhai , Horton Radley M. , Wang Chunyi , Ren Xiaobo 
Published: May 2007

Abstract
Sea ice variability in the North Pacific and its associations with the east Asia–North Pacific winter climate were investigated using observational data. Two dominant modes of sea ice variability in the North Pacific were identified. The first mode features a dipole pattern between the Sea of Okhotsk and the Bering Sea. The second mode is characterized by more uniform ice changes throughout the North Pacific.
Using the principal components of the two dominant modes as the indices (PC1 and PC2), analyses show that the positive phases of PC1 feature a local warming (cooling) in the Sea of Okhotsk (the Bering Sea), which is associated with the formation of the anomalous anticyclone extending from the northern Pacific to Siberia, accompanied by a weakening of the east Asian jet stream and trough. The associated anomalous southeasterlies/easterlies reduce the climatological northwesterlies/westerlies, leading to warm and wet conditions in northeast China and central Siberia. The positive phases of PC2 are characterized by a strong local warming in the northern Pacific that coincides with the anomalous cyclone occupying the entire North Pacific, accompanied by a strengthening of the east Asia jet stream and trough. The associated anomalous northerlies intensify the east Asian winter monsoon (EAWM), leading to cold and dry conditions in the east coast of Asia. The intensified EAWM also strengthens the local Hadley cell, which in turn strengthens the east Asian jet stream and leads to a precipitation deficit over subtropical east Asia. The linkages between PC1 and PC2 and large-scale modes of climate variability were also discussed. It is found that PC1 is a better indicator than the Arctic Oscillation of the recent Siberian warming, whereas PC2 may be a valuable predictor of EAWM.
Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension, and nitric oxide scavenging by cell-free hemoglobin
Authors: Villagra José , Shiva Sruti , Hunter Lori A. , Machado Roberto F. , Gladwin Mark T. , Kato Gregory J. 
Published: May 2007

AbstractIncreased platelet activation is recognized in patients with sickle cell disease (SCD), but its pathogenesis and clinical relevance remain uncertain. Pulmonary arterial hypertension (PAH), an important complication of SCD, is characterized by a proliferative pulmonary vasculopathy, in situ thrombosis, and vascular dysfunction related to scavenging of nitric oxide (NO) by hemoglobin released into blood plasma during intravascular hemolysis. We investigated links between platelet activation, PAH and NO scavenging in patients with SCD. Platelet activation marked by activated fibrinogen receptor correlated to the severity of PAH (r = 0.58, P < .001) and to laboratory markers of intravascular hemolysis, such as reticulocyte count (r = 0.44, P = .02). In vitro exposure of platelets to pathologically relevant concentrations of cell-free hemoglobin promoted basal- and agonist-stimulated activation and blocked the inhibitory effects on platelet activation by an NO donor. In patients with SCD, administration of sildenafil, a phosphodiesterase-5 inhibitor that potentiates NO-dependent signaling, reduced platelet activation (P = .01). These findings suggest a possible interaction between hemolysis, decreased NO bioavailability, and pathologic platelet activation that might contribute to thrombosis and pulmonary hypertension in SCD, and potentially other disorders of intravascular hemolysis. This supports a role for NO-based therapeutics for SCD vasculopathy. This trial was registered at www.clinicaltrials.gov as no. NCT00352430.
Regulation of p73 by Hck through kinase-dependent and independent mechanisms
Authors: Paliwal Preeti , Radha Vegesna , Swarup Ghanshyam 
Published: May 2007

AbstractBackgroundp73, a p53 family member is a transcription factor that plays a role in cell cycle, differentiation and apoptosis. p73 is regulated through post translational modifications and protein interactions. c-Abl is the only known tyrosine kinase that phosphorylates and activates p73. Here we have analyzed the role of Src family kinases, which are involved in diverse signaling pathways, in regulating p73.ResultsExogenously expressed as well as cellular Hck and p73 interactin vivo.In vitrobinding assays show that SH3 domain of Hck interacts with p73. Co-expression of p73 with Hck or c-Src in mammalian cells resulted in tyrosine phosphorylation of p73. Using site directed mutational analysis, we determined that Tyr-28 was the major site of phosphorylation by Hck and c-Src, unlike c-Abl which phosphorylates Tyr-99. In a kinase dependent manner, Hck co-expression resulted in stabilization of p73 protein in the cytoplasm. Activation of Hck in HL-60 cells resulted in tyrosine phosphorylation of endogenous p73. Both exogenous and endogenous Hck localize to the nuclear as well as cytoplasmic compartment, just as does p73. Ectopically expressed Hck repressed the transcriptional activity of p73 as determined by promoter assays and semi-quantitative RT-PCR analysis of the p73 target, Ipaf and MDM2. SH3 domain- dependent function of Hck was required for its effect on p73 activity, which was also reflected in its ability to inhibit p73-mediated apoptosis. We also show that Hck interacts with Yes associated protein (YAP), a transcriptional co-activator of p73, and shRNA mediated knockdown of YAP protein reduces p73 induced Ipaf promoter activation.ConclusionWe have identified p73 as a novel substrate and interacting partner of Hck and show that it regulates p73 through mechanisms that are dependent on either catalytic activity or protein interaction domains. Hck-SH3 domain-mediated interactions play an important role in the inhibition of p73-dependent transcriptional activation of a target gene, Ipaf, as well as apoptosis.
Front-line Bevacizumab in combination with Oxaliplatin, Leucovorin and 5-Fluorouracil (FOLFOX) in patients with metastatic colorectal cancer: a multicenter phase II study
Authors: Emmanouilides Christos , Sfakiotaki Georgia , Androulakis Nikolaos , Kalbakis Kostas , Christophylakis Charalambos , Kalykaki Antonia , Vamvakas Lambros , Kotsakis Athanasios , Agelaki Sofia , Diamandidou Eleni , Touroutoglou Nikolaos , Chatzidakis Adam , Georgoulias Vassilis , Mavroudis Dimitris , Souglakos John 
Published: May 2007

Abstract

Purpose
To evaluate the efficacy and the toxicity of front line FOLFOX4 combined with bevacizumab in patients with metastatsic CRC (mCRC).

Patients and Methods
Chemotherapy-naïve patients with mCRC, received bevacizumab (5 mg/kg every 2 weeks d1), oxaliplatin (85 mg/m2 on d1), leucovorin (200 mg/m2) on days 1 and 2 and 5-Fluorouracil (400 mg/m2 as i.v. bolus and 600 mg/m2 as 22 h i.v. continuous infusion on days 1 and 2) every 2 weeks.

Results
Fifty three patients (46 with a PS 0–1) were enrolled. Complete and partial response was achieved in eight (15.1%) and 28 (52.8%) patients, respectively (ORR: 67.9%; 95% C.I.: 53.8%–92%); 11 (20.7%) patients had stable disease and six (11.3%) progressive disease. With a median follow up period of 13.5 months, time to tumor progression was 11 months while the median survival has not yet been reached; the probability of 1-, 2- and 3- year survival was 79.8%, 63.8% and 58.3%, respectively; Two patients relapsed during the follow up period. Eight (15%) patients underwent metastasectomy with R0 resections. Grade 3–4 neutropenia occurred in 15.1% of patients and one (1.9%) of them presented febrile neutropenia. Non-hematologic toxicity included grade 3 diarrhea (7.6%) and grade 2 and 3 neurotoxicity in 16.9 and 15.1% of patients, respectively. One (1.9%) patient presented pulmonary embolism and one (1.9%) cardiac ischaemia. There was one (1.9%) sudden death after the first cycle.

Conclusion
The combination of FOLFOX4/bevacizumab appears to be highly effective, well tolerated and merits further evaluation in patients with mCRC.
Differences in the quality of primary medical care for CVD and diabetes across the NHS: evidence from the quality and outcomes framework
Authors: McLean Gary , Guthrie Bruce , Sutton Matt 
Published: May 2007

Abstract
Background
Health policy in the UK has rapidly diverged since devolution in 1999. However, there is relatively little comparative data available to examine the impact of this natural experiment in the four UK countries. The Quality and Outcomes Framework of the 2004 General Medical Services Contract provides a new and potentially rich source of comparable clinical quality data through which we compare quality of primary medical care for coronary heart disease (CHD), stroke, hypertension and diabetes across the four UK countries.

Methods
A cross-sectional analysis was undertaken involving 10,064 general practices in England, Scotland, Wales and Northern Ireland. The main outcome measures were prevalence rates for CHD, stroke, hypertension and diabetes. Achievement on 14 simple process, 3 complex process, 9 intermediate outcome and 5 treatment indicators for the four clinical areas.

Results
Prevalence varies by up to 28% between the four UK countries, which is not reflected in resource distribution between countries, and penalises practices in the high prevalence countries (Wales and Scotland). Differences in simple process measures across countries are small. Larger differences are found for complex process, intermediate outcome and treatment measures, most notably for Wales, which has consistently lower quality of care. Scotland has generally higher quality than England and Northern Ireland is most consistently the highest quality.

Conclusion
Previously identified weaknesses in Wales related to waiting times appear to reflect a more general quality problem within NHS Wales. Identifying explanations for the observed differences is limited by the lack of comparable data on practice resources and organisation. Maximising the value of cross-jurisdictional comparisons of the ongoing natural experiment of health policy divergence within the UK requires more detailed examination of resource and organisational differences.
Case control study of the geographic variability of exposure to disinfectant byproducts and risk for rectal cancer
Authors: Bove Gerald E , Rogerson Peter A , Vena John E 
Published: May 2007

Abstract

Background
Levels of byproducts that result from the disinfection of drinking water vary within a water distribution system. This prompted us to question whether the risk for rectal cancer also varies, depending upon one's long term geographic location within the system. Such a geographic distribution in rectal cancer risk would follow naturally from an association between level of byproduct and rectal cancer risk. We assess the effects of estimated geographic variability in exposure to some of the components of the trihalomethane group of disinfectant byproducts (DBPs) on the odds ratios and probabilities for rectal cancer in white males in a case control study of 128 cases and 253 controls, conducted in Monroe County, Western New York State, U.S.A. The study was designed around health data initially collected at the University at Buffalo (Department of Social and Preventative Medicine) as part of the Upstate New York Diet Study, and trihalomethane (THM) data collected from a separate independent study of THMs conducted by Monroe County Department of Health. Case participants were chosen from hospital pathology records. The controls are disease-free white males between 35–90 years old, living in Monroe County, and chosen from control groups for studies from cancer of five other (unrelated) sites. Using a combination of case control methodology and spatial analysis, the spatial patterns of THMs and individual measures of tap water consumption provide estimates of the effects of ingestion of specific amounts of some DBPs on rectal cancer risk. Trihalomethane (THM) data were used to spatially interpolate levels at the taps of cases and controls, and odds ratios were estimated using logistic regression to assess the effects of estimated THM exposure dose on cancer risk, adjusting for alcohol, dietary beta carotene intake, tap water intake, and total caloric intake.

Results
Trihalomethane levels varied spatially within the county; although risk for rectal cancer did not increase with total level of trihalomethanes, increasing levels of the component bromoform (measured in ug/day) did correspond with an increase in odds ratios (OR = 1.85; 95% CI = 1.25 – 2.74) for rectal cancer. The highest quartiles of estimated consumption of bromoform (1.69–15.43 ug/day) led to increased risk for rectal cancer (OR = 2.32; 95% CI = 1.22–4.39). Two other THMs were marginally associated with an increase in risk – chlorodibromomethane (OR = 1.78, 95% CI = 1.00–3.19) and bromodichloromethane (OR = 1.15; 95% CI = 1.00–1.32).

Conclusion
Levels of THMs in the water distribution system exhibited spatial variation that was partially due to variation in water age. We also observed a geographic pattern of increased risk of rectal cancer in areas with the highest levels of bromoform in the county.
Developmental expression of DAX1 in the European sea bass, Dicentrarchus labrax: lack of evidence for sexual dimorphism during sex differentiation
Authors: Martins Rute ST , Deloffre Laurence AM , Mylonas Constantinos C , Power Deborah M , Canário Adelino VM 
Published: May 2007

Abstract

Background
DAX1 (NR0B1), a member of the nuclear receptors super family, has been shown to be involved in the genetic sex determination and in gonadal differentiation in several vertebrate species. In the aquaculture fish European sea bass, Dicentrarchus labrax, and in the generality of fish species, the mechanisms of sex determination and differentiation have not been elucidated. The present study aimed at characterizing the European DAX1 gene and its developmental expression at the mRNA level.

Methods
A full length European sea bass DAX1 cDNA (sbDAX1) was isolated by screening a testis cDNA library. The structure of the DAX1 gene was determined by PCR and Southern blot. Multisequence alignments and phylogenetic analysis were used to compare the translated sbDAX1 product to that of other vertebrates. sbDAX1 expression was analysed by Northern blot and relative RT-PCR in adult tissues. Developmental expression of mRNA levels was analysed in groups of larvae grown either at 15°C or 20°C (masculinising temperature) during the first 60 days, or two groups of fish selected for fast (mostly females) and slow growth.

Results
The sbDAX1 is expressed as a single transcript in testis and ovary encoding a predicted protein of 301 amino acids. A polyglutamine stretch of variable length in different DAX1 proteins is present in the DNA binding domain. The sbDAX1 gene is composed of two exons, separated by a single 283 bp intron with conserved splice sites in same region of the ligand binding domain as other DAX1 genes. sbDAX1 mRNA is not restricted to the brain-pituitary-gonadal axis and is also detected in the gut, heart, gills, muscle and kidney. sbDAX1 mRNA was detected as early as 4 days post hatching (dph) and expression was not affected by incubation temperature. Throughout gonadal sex differentiation (60–300 dph) no dimorphic pattern of expression was observed.

Conclusion
The sbDAX1 gene and putative protein coding region is highly conserved and has a wide pattern of tissue expression. Although gene expression data suggests sbDAX1 to be important for the development and differentiation of the gonads, it is apparently not sex specific.
Association of TP53codon 72 polymorphism and the outcome of adjuvant therapy in breast cancer patients
Authors: Toyama Tatsuya , Zhang Zhenhuan , Nishio Mariko , Hamaguchi Maho , Kondo Naoto , Iwase Hirotaka , Iwata Hiroji , Takahashi Satoru , Yamashita Hiroko , Fujii Yoshitaka 
Published: May 2007

Abstract
Introduction
Single-nucleotide polymorphisms (SNPs) in codon 72 of the TP53 (also known as p53) gene (rs1042522) and in the promoter region of the MDM2 gene (SNP309; rs2279744) have been suggested to play roles in many cancers. We investigated whether these SNPs were associated with patient outcome and the effect of adjuvant systemic therapy.

Methods
The genotypes of TP53 codon 72 and MDM2 SNP309 were defined among 557 primary Japanese breast cancer patients (median follow-up, 61.7 months). The effects of several variables on survival were tested by Cox's proportional hazards regression analysis.

Results
We showed that the Pro/Pro genotype of TP53 codon 72 was associated with poorer disease-free survival (DFS) than other genotypes by Kaplan-Meier analysis (P = 0.049) and multivariate Cox's proportional hazards regression analysis (P = 0.047, risk ratio of recurrence = 1.67), whereas MDM2 SNP309 status was not associated with DFS. The association of the Pro/Pro TP53 genotype with poorer DFS was especially significant in patients who received adjuvant chemotherapy (P = 0.009). In contrast, among the patients who had received adjuvant hormonal therapy or no adjuvant systemic therapy, TP53 codon 72 genotype was not associated with DFS.

Conclusion
The Pro/Pro genotype of TP53 codon 72 appears to be an independent prognostic marker in breast cancer patients.
Expression and translocation of fluorescent-tagged p21-activated kinase-binding domain and PH domain of protein kinase B during murine neutrophil chemotaxis
Authors: Magalhães Marco A O , Zhu Fei , Sarantis Helen , Gray-Owen Scott D , Ellen Richard P , Glogauer Michael 
Published: May 2007

AbstractNeutrophils are key cells of the innate immune system; they are terminally differentiated and therefore difficult to genetically manipulate and study in vitro. In the present study, we describe a protocol to transiently express two fluorescent markers, the PH domain of protein kinase B fused to red fluorescent protein and the p21-activated kinase-binding domain fused to a yellow fluorescent protein, in primary neutrophils. Using this approach, we are able to achieve a transfection efficiency of ∼30%. The expression of the transfected probes occurred within 2 h and allowed for real-time monitoring of intermediates in key neutrophil activation pathways at the leading edge of migrating cells. We describe here a transfection protocol for primary neutrophils, which preserves fMLP-mediated cell polarization and cytoskeleton reorganization with simultaneous accumulation of PI-3K products and active Rac at the leading edge. The visualization and analysis of transfected fluorescent markers in primary neutrophils are a powerful technique to monitor chemotaxis signaling pathways in real time.