bluuebunny/crossref_metadata_2025_split huggingface dataset:
Raising Pupils' Self‐Esteem through Leadership Activities in Art
Authors: Hickman Richard 
Published: October 2006

AbstractThis article is about the ways in which young people who have disengaged from learning in school can find a way back through leadership activities in art. It is based on a project which was funded by a small grant from the Wallenberg Foundation. The project explored the potential of an approach to developing positive leadership qualities in pupils who were not consistently committed to the school's learning purposes. This account describes and comments upon two pupils' guided attempts at peer teaching in art and its subsequent effect upon their selfesteem and attitude towards school. It was found that pupils who taught art to other pupils had an increased sense of self‐worth and were more positively affected towards learning. However, broader issues, such as the negative nature of some school systems and their role in de‐motivating pupils were highlighted.
Elementary Grade Retention in Texas and Reading Achievement Among Racial Groups: 1994–2002
Authors: Lorence Jon , Dworkin Anthony Gary 
Published: October 2006

AbstractStudents who failed the Texas mandatory third grade reading test were followed through their sophomore year in high school. Comparisons of reading scores between third grade students who repeated the grade and their socially promoted classmates revealed that the positive effect of retention persisted over time. Retention in third grade benefited low‐performing readers regardless of race. Supplemental analyses found that the results are not likely attributable to selection biases, differential attrition of students from the panel, changes in the special education status of students, and regression to the mean effects. Making students repeat a grade, when supplemented with additional educational assistance, can benefit academically challenged children.
Cutaneous granular cell tumor with epidermal involvement: a potential mimic of melanocytic neoplasia
Authors: Ray Sourav , Jukic Drazen M. 
Published: October 2006

Background:  Cutaneous granular cell tumor (GCT) may present with extension into the junctional region of the epidermis and thus may mimic melanocytic neoplasms.Methods:  We reviewed three cases of cutaneous GCT where a melanocytic neoplasm was either initially diagnosed or considered in the differential diagnosis. Histopathology was evaluated in regards to features associated with melanocytic neoplasms. Immunohistochemistry was performed to delineate a panel useful in the distinction of these and other entities.Results:  All cases consisted of spindle and epithelioid cells with granular cytoplasm and bland nuclei and were centered in the superficial dermis with extension into the epidermis. Two cases resembled Spitz nevi and one case demonstrated lentiginous growth. All cases stained positively with calretinin and inhibin. Two of the three cases stained diffusely with S100 and 2/2 cases with CD56. HAM56 and CD68 were positive in one case and another showed positivity for NSE and PGP9.5. HMB‐45, tyrosinase, and Melan‐A were non‐reactive in all cases tested.Conclusions:  GCT may involve the epidermis and has a growth pattern similar to melanocytic neoplasms. An immunohistochemical (IHC) panel including S100, Melan‐A, tyrosinase, HMB‐45, CD56, CD68, calretinin, inhibin, and PGP9.5 may aid in the distinction and may spare the patient from unnecessary morbidity.
Reduced expression of the antiapoptotic proteins of Bcl‐2 gene family in the lesional epidermis of patients with Darier’s disease
Authors: Pasmatzi Efi , Badavanis George , Monastirli Alexandra , Tsambaos Dionysios 
Published: October 2006

Background:  Dyskeratotic cells in Darier’s disease (DD) are thought to represent apoptotic keratinocytes. Bcl‐2 gene family proteins play a major role in the regulation of apoptosis of epidermal keratinocytes and reveal pleiotropic interactions with intracellular Ca2+ homeostasis. The latter is impaired in DD because of mutations of ATP2A2 gene that encodes the type 2 isoforms of the sarcoplasmic/endoplasmic reticulum (ER) Ca++ ATPase 2 (SERCA2) pump.Methods:  The expression of Bcl‐2, Bax, and Bcl‐xL proteins was investigated in the epidermis of 11 patients with DD and of 11 sex‐ and age‐matched healthy controls by immunohistochemistry.Results:  The expression of Bcl‐2 and Bcl‐xL was clearly reduced in the lesional epidermis of the patients, as compared with the normal epidermis of healthy controls, whereas the expression of Bax remained unaltered.Conclusions:  The alterations in the expression of Bcl‐2 gene family proteins could be a crucial event for the activation of the apoptotic process in the lesional epidermis of DD patients and for the occurrence of the characteristic dyskeratotic keratinocytes. The question as to whether these alterations are associated with the ER Ca2+ depletion in DD or represent secondary phenomena unrelated to the genetic defect of this genodermatosis remains to be elucidated.
Supplementary effect of peppermint (Mentha × piperita) on dry matter intake, digestibility, ruminal fermentation and milk production in early lactating dairy cows
Authors: HOSODA Kenji , MATSUYAMA Hiroki , PARK Woong‐Yeoul , NISHIDA Takehiro , ISHIDA Motohiko 
Published: October 2006

ABSTRACTThe aim of this research was to investigate the effect of peppermint feeding on dry matter intake, nutrient digestibility, ruminal fermentation and milk production in early lactating cows. Four Holstein cows were offered a diet with 5% dried peppermint and four Holstein cows remained on a diet without 5% of dried peppermint on a dry matter basis. The addition of peppermint to feed did not affect dry matter intake, although the eating time of feed was increased by mixing the feed with peppermint. There were no significant differences in the nutrient digestibilities between the two treatments. The ruminal ammonia and volatile fatty acids concentrations were similar in the two treatments, however, peppermint ingestion by cows led to a decrease in ruminal pH. The lowered pH value was within the stable pH condition range. No significant differences in the treatments were observed in milk production or milk composition except for the milk fat content. These results suggest that feeding peppermint to early lactating cows had little effect on their dry matter intake, nutrient digestibility, ruminal fermentation and milk production.
Variations of blood corpuscles and immunoglobulin G of plasma and milk in placenta‐fed sows
Authors: LEE Sung‐Dae , SONODA Tatsunobu , HASEGAWA Nobumi , FUKUNAGA Rie , HARADA Hiroshi , SONG Young‐Min , HIDAKA Ryoichi 
Published: October 2006

ABSTRACTThe objective of the present study was to investigate the variations of blood corpuscle and immunoglobulin G (IgG) of plasma and milk in sows fed placenta. The first experiment was designed with two groups, the experimental group (Ex: with placenta) and the control (Ct: without placenta) depending on whether they were fed placenta or not. The second experiment was designed using varying condition of placenta (fresh: Fp or cooked: Cp) and amounts of placenta (900 g or 1800 g) according to 2 × 2 factorial design. The number of erythrocytes were higher (P < 0.05) in Ct than in Ex on day 1 of Experiment 1. It was greater in Fp than in Cp on day 3 (P < 0.01) and day 8 (P < 0.05) in Experiment 2. The concentration of hemoglobin was greater (P < 0.05) in Fp than in Cp on day 3 in Experiment 2. In Experiment 1, the concentration of IgG in milk was significantly greater in Ex than in Ct on day 15 (P < 0.05) and day 20 (P < 0.01). In Experiment 2, the concentration of IgG in plasma was significantly greater in Fp than in Cp from day 3 to day 20 (P < 0.05). The concentration of IgG in milk was greater (P < 0.05) in Fp than in Cp on day 1 in Experiment 2. The results indicate that, when supplied as a feed, fresh placenta affected the IgG in the plasma and milk of sows.
Ploidy level interacts with population size and habitat conditions to determine the degree of herbivory damage in plant populations
Authors: Münzbergová Zuzana 
Published: October 2006

Recently it has been suggested that ploidy level of a plant population may have important effects on plant‐animal interactions. Plant‐animal interactions can also be strongly altered by factors such as plant population size and habitat conditions. It is, however, not known how these factors interact to shape the overall pattern of plant‐animal interactions.I studied the interaction between a perennial plant, Aster amellus, and a monophagous herbivorous moth, Coleophora obscenella, and investigated the effect of ploidy level of the plant population, plant population size, isolation and habitat conditions on density of the insect, damage by the insect, and plant performance.Ploidy level, plant population size and habitat conditions, but not isolation, strongly influence plant‐herbivore interactions. Furthermore, there are significant interactions between effects of ploidy level and plant population size and between ploidy level and isolation. Hexaploid plants suffer higher seed damage by the herbivore, but their seed production is still higher than that of diploids. Herbivores thus partly limit the evolutionary success of the hexaploid plants. Plant‐animal interactions are also strongly determined by plant population size. Small populations of A. amellus (below forty flowering ramets) host no C. obscenella larvae, indicating a minimum A. amellus population size that can sustain a viable C. obscenella population. Negative and positive effects of plant population size balance and result in no relationship between plant population size and number of developed seeds per flower head. The results also show a significant interaction between ploidy level and plant population size, indicating that the increase in density of C. obscenella larvae with plant population size is greater in hexaploid than in diploid populations. The results also indicate that the effect of ploidy level on plant‐herbivore interactions can be altered by plant population size, which suggests that plant‐herbivore interactions are driven by a complex of interactions among different factors. Studying each factor separately could thus lead to biased conclusions about patterns of interactions in such systems.
Identification and Characterization of a Major Cell Wall-Associated Iron-Regulated Envelope Protein (Irep-28) in
Mycobacterium tuberculosis
Authors: Yeruva Veena C. , Duggirala Sridevi , Lakshmi V. , Kolarich Daniel , Altmann Friedrich , Sritharan Manjula 
Published: October 2006

ABSTRACT

Iron limitation and the expression of mycobactin and carboxymycobactin by
Mycobacterium tuberculosis
are known. Here, we report how iron regulated the coordinate expression of these two siderophores and a 28-kDa cell wall-associated iron-regulated protein (Irep-28). Irep-28 is identified as the DNA-binding HU homologue HupB protein (
hupB
[Rv2986c]). Antibodies to this protein were detected in sera from tuberculosis patients. The location of the protein in the cell wall makes it a potential drug target.
Data Quality of the Meteo-France C-Band Polarimetric Radar
Authors: Gourley Jonathan J. , Tabary Pierre , Parent du Chatelet Jacques 
Published: October 2006

Abstract
The French operational radar network is being upgraded and expanded from 2002 to 2006 by Meteo-France in partnership with the French Ministry of the Environment. A detailed examination of the quality of the raw polarimetric variables is reported here. The analysis procedures determine the precision of the measurements and quantify errors resulting from miscalibration, near-radome interference, and noise effects. Correction methods to remove biases resulting from effective noise powers in the horizontal and vertical channels, radar miscalibration, and the system offset in differential propagation phase measurements are presented and evaluated. Filtering methods were also required in order to remove azimuthal dependencies discovered with fields of differential reflectivity and differential propagation phase. The developed data quality analysis procedures may be useful to the agencies that are in the process of upgrading their radar networks with dual-polarization capabilities.
B-cell extrinsic CR1/CR2 promotes natural antibody production and tolerance induction of anti-αGAL–producing B-1 cells
Authors: Shimizu Ichiro , Kawahara Toshiyasu , Haspot Fabienne , Bardwell Philip D. , Carroll Michael C. , Sykes Megan 
Published: October 2006

AbstractB-1b cells produce IgM natural antibodies against α1-3Galβ1-4GlcNAc (αGal). These can be tolerized by nonmyeloablative induction of mixed chimerism using αGal-positive (αGal+) donor marrow. We assessed the role of CR1/2 in this model for induction of tolerance of B-1b cells. Mixed hematopoietic chimerism was induced in α1-3galactosyltransferase (GalT−/−) and GalT−/−Cr2−/− mice with αGal+ BALB/c marrow donors. Anti-αGal Ab and anti-αGal Ab–producing B cells became undetectable in GalT−/− chimeras, whereas they persisted in chimeric GalT−/−Cr2−/− mice. To determine whether CR1/2 expression on stromal cells and/or hematopoietic cells was critical for B-1–cell tolerance, we generated GalT−/− radiation chimeras in which CR1/CR2 was expressed on either stromal cells, hematopoietic cells, neither, or both. After induction of mixed chimerism from αGal+ allogeneic bone marrow (BM) donors, anti-αGal–producing B cells were rendered tolerant in reconstituted recipients expressing only stromal CR1/CR2. Our results suggest a possible role for follicular dendritic cells that pick up immune complexes via CR1/CR2 receptors in the tolerization of B-1b cells.
Skeleton-binding protein 1 functions at the parasitophorous vacuole membrane to traffic PfEMP1 to the Plasmodium falciparum–infected erythrocyte surface
Authors: Maier Alexander G. , Rug Melanie , O'Neill Matthew T. , Beeson James G. , Marti Matthias , Reeder John , Cowman Alan F. 
Published: October 2006

Abstract
A key feature of Plasmodium falciparum, the parasite causing the most severe form of malaria in humans, is its ability to export parasite molecules onto the surface of the erythrocyte. The major virulence factor and variant surface protein PfEMP1 (P falciparum erythrocyte membrane protein 1) acts as a ligand to adhere to endothelial receptors avoiding splenic clearance. Because the erythrocyte is devoid of protein transport machinery, the parasite provides infrastructure for trafficking across membranes it traverses. In this study, we show that the P falciparum skeleton-binding protein 1 (PfSBP1) is required for transport of PfEMP1 to the P falciparum–infected erythrocyte surface. We present evidence that PfSBP1 functions at the parasitophorous vacuole membrane to load PfEMP1 into Maurer clefts during formation of these structures. Furthermore, the major reactivity of antibodies from malaria-exposed multigravid women is directed toward PfEMP1 because this is abolished in the absence of PfSBP1.
The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization
Authors: Baschal Erin E , Chen Kuan J , Elliott Lee G , Herring Matthew J , Verde Shawn C , Wolkow Tom D 
Published: October 2006

AbstractBackgroundDNA structure checkpoints are conserved eukaryotic signal transduction pathways that help preserve genomic integrity. Upon detecting checkpoint signals such as stalled replication forks or double-stranded DNA breaks, these pathways coordinate appropriate stress responses. Members of the PI-3 kinase related kinase (PIKK) family are essential elements of DNA structure checkpoints. In fission yeast, the Rad3 PIKK and its regulatory subunit Rad26 coordinate the detection of checkpoint signals with pathway outputs.ResultsWe found that untreatedrad26Δcells were defective for two microtubule-dependent processes: chromosome segregation and morphogenesis. Interestingly, cytoplasmic accumulation of Rad26-GFP occurred following treatment with microtubule destabilizing drugs, but not during treatment with the genotoxic agent Phleomycin. Cytoplasmic accumulation of Rad26-GFP depended on Rad24, a 14-3-3 protein also required for DNA structure checkpoints and morphogenesis. Results of over expression and epistasis experiments confirm that Rad26 and Rad24 define a response to microtubule destabilizing conditions.ConclusionTwo DNA structure checkpoint proteins with roles in morphogenesis define a response to microtubule destabilizing conditions.
Dog Y chromosomal DNA sequence: identification, sequencing and SNP discovery
Authors: Natanaelsson Christian , Oskarsson Mattias CR , Angleby Helen , Lundeberg Joakim , Kirkness Ewen , Savolainen Peter 
Published: October 2006

Abstract

Background
Population genetic studies of dogs have so far mainly been based on analysis of mitochondrial DNA, describing only the history of female dogs. To get a picture of the male history, as well as a second independent marker, there is a need for studies of biallelic Y-chromosome polymorphisms.
However, there are no biallelic polymorphisms reported, and only 3200 bp of non-repetitive dog Y-chromosome sequence deposited in GenBank, necessitating the identification of dog Y chromosome sequence and the search for polymorphisms therein. The genome has been only partially sequenced for one male dog, disallowing mapping of the sequence into specific chromosomes. However, by comparing the male genome sequence to the complete female dog genome sequence, candidate Y-chromosome sequence may be identified by exclusion.

Results
The male dog genome sequence was analysed by Blast search against the human genome to identify sequences with a best match to the human Y chromosome and to the female dog genome to identify those absent in the female genome. Candidate sequences were then tested for male specificity by PCR of five male and five female dogs.
32 sequences from the male genome, with a total length of 24 kbp, were identified as male specific, based on a match to the human Y chromosome, absence in the female dog genome and male specific PCR results. 14437 bp were then sequenced for 10 male dogs originating from Europe, Southwest Asia, Siberia, East Asia, Africa and America. Nine haplotypes were found, which were defined by 14 substitutions. The genetic distance between the haplotypes indicates that they originate from at least five wolf haplotypes. There was no obvious trend in the geographic distribution of the haplotypes.

Conclusion
We have identified 24159 bp of dog Y-chromosome sequence to be used for population genetic studies. We sequenced 14437 bp in a worldwide collection of dogs, identifying 14 SNPs for future SNP analyses, and giving a first description of the dog Y-chromosome phylogeny.
Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12
Authors: Hayes Everett T , Wilks Jessica C , Sanfilippo Piero , Yohannes Elizabeth , Tate Daniel P , Jones Brian D , Radmacher Michael D , BonDurant Sandra S , Slonczewski Joan L 
Published: October 2006

Abstract

Background
In Escherichia coli, pH regulates genes for amino-acid and sugar catabolism, electron transport, oxidative stress, periplasmic and envelope proteins. Many pH-dependent genes are co-regulated by anaerobiosis, but the overall intersection of pH stress and oxygen limitation has not been investigated.

Results
The pH dependence of gene expression was analyzed in oxygen-limited cultures of E. coli K-12 strain W3110. E. coli K-12 strain W3110 was cultured in closed tubes containing LBK broth buffered at pH 5.7, pH 7.0, and pH 8.5. Affymetrix array hybridization revealed pH-dependent expression of 1,384 genes and 610 intergenic regions. A core group of 251 genes showed pH responses similar to those in a previous study of cultures grown with aeration. The highly acid-induced gene yagU was shown to be required for extreme-acid resistance (survival at pH 2). Acid also up-regulated fimbriae (fimAC), periplasmic chaperones (hdeAB), cyclopropane fatty acid synthase (cfa), and the "constitutive" Na+/H+ antiporter (nhaB). Base up-regulated core genes for maltodextrin transport (lamB, mal), ATP synthase (atp), and DNA repair (recA, mutL). Other genes showed opposite pH responses with or without aeration, for example ETS components (cyo,nuo, sdh) and hydrogenases (hya, hyb, hyc, hyf, hyp). A hypF strain lacking all hydrogenase activity showed loss of extreme-acid resistance. Under oxygen limitation only, acid down-regulated ribosome synthesis (rpl,rpm, rps). Acid up-regulated the catabolism of sugar derivatives whose fermentation minimized acid production (gnd, gnt, srl), and also a cluster of 13 genes in the gadA region. Acid up-regulated drug transporters (mdtEF, mdtL), but down-regulated penicillin-binding proteins (dacACD, mreBC). Intergenic regions containing regulatory sRNAs were up-regulated by acid (ryeA, csrB, gadY, rybC).

Conclusion
pH regulates a core set of genes independently of oxygen, including yagU, fimbriae, periplasmic chaperones, and nhaB. Under oxygen limitation, however, pH regulation is reversed for genes encoding electron transport components and hydrogenases. Extreme-acid resistance requires yagU and hydrogenase production. Ribosome synthesis is down-regulated at low pH under oxygen limitation, possibly due to the restricted energy yield of catabolism. Under oxygen limitation, pH regulates metabolism and transport so as to maximize alternative catabolic options while minimizing acidification or alkalinization of the cytoplasm.
Trends in scientific activity addressing transmissible spongiform encephalopathies: a bibliometric study covering the period 1973–2002
Authors: Sanz-Casado Elías , Ramírez-de Santa Pau Margarita , Suárez-Balseiro Carlos A , Iribarren-Maestro Isabel , de Pedro-Cuesta Jesús 
Published: October 2006

Abstract

Background
The purpose of this study is to analyse the trends in scientific research on transmissible spongiform encephalopathies by applying bibliometric tools to the scientific literature published between 1973 and 2002.

Methods
The data for the study were obtained from Medline database, in order to determine the volume of scientific output in the above period, the countries involved, the type of document and the trends in the subject matters addressed. The period 1973–2002 was divided in three sub-periods.

Results
We observed a significant growth in scientific production. The percentage of increase is 871.7 from 1973 to 2002. This is more evident since 1991 and particularly in the 1996–2001 period. The countries found to have the highest output were the United States, the United Kingdom, Japan, France and Germany. The evolution in the subject matters was almost constant in the three sub-periods in which the study was divided. In the first and second sub-periods, the subject matters of greatest interest were more general, i.e Nervous system or Nervous system diseases, Creutzfeldt-Jakob disease, Scrapie, and Chemicals and Drugs, but in the last sub-period, some changes were observed because the Prion-related matters had the greatest presence.
Collaboration among authors is small from 1973 to 1992, but increases notably in the third sub-period, and also the number of authors and clusters formed. Some of the authors, like Gajdusek or Prusiner, appear in the whole period.

Conclusion
The study reveals a very high increase in scientific production. It is related also with the beginnings of research on bovine spongiform encephalopathy and variant Creutzfeldt-Jakob disease, with the establishment of progressive collaboration relationships and a reflection of public health concerns about this problem.