bluuebunny/crossref_metadata_2025_split huggingface dataset:
How do dams affect freshwater fish distributions in Japan? Statistical analysis of native and nonnative species with various life histories
Authors: Han Mideok , Fukushima Michio , Kameyama Satoshi , Fukushima Takehiko , Matsushita Bunkei 
Published: October 2007

AbstractWe examined the effects of dams on freshwater fish species based on data collected during 1990–2004 from 200 drainage systems in Japan. Of the 76 fish species examined, the occurrence of 20 species within Petromyzontidae, Cyprinidae, Cobitididae, Salmonidae, Cottidae, and Gobiidae was negatively affected by the presence of dams located in the downstream reaches of fish survey sites, whereas the occurrence of 12 species within Cyprinidae, Adrianichthyidae, Centrarchidae, and Gobiidae was positively associated with the presence of dams. A significantly higher proportion of the fishes with a negative damming effect were diadromous species as compared to the fishes with a positive damming effect. Conversely, the latter group had a significantly higher proportion of nonnative species than the former. A significant interaction existed between the effects of damming and the effects of elevation on family‐specific species richness. Families dominated by native migratory species showed a greater reduction in the number of species above dams at lower elevations, whereas families represented primarily by nonnative species had higher species richness above dams at higher elevations, except for Centrarchidae, which was always higher in species richness above dams regardless of elevation. Based on our findings, dams in Japan have adversely affected native freshwater fishes by blocking their migration routes, favoring nonnative fishes, or altering existing habitats.
Characterization and Compositional Studies of Oil from Seeds of Desi Chickpea (Cicer arietinum L.) Cultivars Grown in Pakistan
Authors: Zia‐Ul‐Haq Muhammad , Ahmad Mansoor , Iqbal Shahid , Ahmad Shakeel , Ali Hakoomat 
Published: October 2007

AbstractThe physiochemical properties and fatty acid (FA) composition of oil from seeds of four desi chickpea cultivars, grown in Pakistan, were investigated. The oil content was relatively low (5.88–6.87%). The physiochemical parameters determined included refractive indices (RI) at 40 °C (1.48–1.49), relative density (0.95–0.96), iodine value (IV) (111.87–113.69), acid value (AV) (2.55–2.73 mg KOH/g), saponification value (SV) (183.98–185.64 mg KOH/g), unsaponifiable matter (UM) (2.99–3.71%), peroxide value (PV) (3.97–6.37 mequiv/Kg), p‐anisidine value (p‐AV) (5.39–8.74), and oxidation value (OV) (13.67–22.34). Linoleic acid and oleic acid were the dominant FAs. Results from most of the parameters revealed significant (P < 0.05) differences among the cultivars. The findings reveal Desi chickpea (Cicer arietinum L.), indigenous to Pakistan, to be a potentially valuable legume crop with comparable nutritional quality oil.
Full‐Press Oil Extraction of Cuphea (PSR23) Seeds
Authors: Evangelista Roque L. , Cermak Steven C. 
Published: October 2007

AbstractCuphea PSR23, a semi‐domesticated, high‐capric‐acid hybrid from Cuphea viscosissima × Cuphea lanceolata, is being developed as a potential commercial alternative source of medium‐chain fatty acids. The present study evaluated the effects of initial seed moisture and final moisture contents of cooked flaked seed on Cuphea’s pressing characteristics and the quality of the extracted oil. Seeds with 9 and 12% initial moisture contents (MC) were flaked and cooked at different residence times to produce cooked seeds with MC of 3.0–5.5%. Cooked seeds were pressed using a laboratory screw press. Eighty and 84% oil were extracted from cooked seeds with 5.5 and 3.0% MC, respectively. The seeds with 9% initial MC exhibited lower pressing load increase (9.1 per 1% decrease in MC) than the seeds with 12% initial MC (16.4 per 1% decrease in MC). The pressing rate decreased by 3% as the cooked flaked seed MC decreased. The amount of foots in the oil increased from 3 to 6.6% and chlorophyll content increased from 200 to 260 ppm as cooked flaked seed MC decreased from 5.5 to 3.0%. FFA contents were 2.5% for all treatments MC studied. The phosphatide content increased as the cooked flaked seed MC decreased but the amounts were still within the levels of water‐degummed oil.
Heat Treatment Improves Olive Oil Extraction
Authors: Cruz Sergio , Yousfi Khaled , Oliva Jesús , García José M. 
Published: October 2007

AbstractTo determine the effect of hot water pre‐treatment on olive oil extraction, six cultivars of olive fruit (Olea europaea L. cvs. “Arbequina”, “Hojiblanca”, “Lechín”, “Manzanilla”, “Picual”, and “Verdial”) were heated at 50, 55, and 60 °C prior to laboratory scale oil extraction. Heat treatment resulted in higher oil extraction than unheated control samples. Quality parameters of the oils were not significantly (P < 0.05) affected by these treatments; however, oil stability and bitterness intensity were reduced and pigment content was increased through pre‐heat treatment. This process may be incorporated economically into olive oil processing.
Pseudoparalysis in the Pavlik Harness: Beware of septic arthritis
Authors: Stevenson Andrew J. , Shewalle Sandeep B. , Clarke Nicholas M. P. 
Published: October 2007

Purpose Pseudoparalysis of the leg occurring during use of the Pavlik Harness (PH) is a seldom-reported condition. Three cases of pseudoparalysis are reported here to illustrate the need for careful assessment of this condition. Methods A series of patients using the PH and presenting with pseudoparalysis was compiled following a retrospective review of case notes. Results In 3 cases, infants treated for developmental dysplasia of the hip using the PH were found to have developed a pseudoparalysis of the affected limb. All presented with similar signs, with the infant being systemically irritable and reluctant to move the limb. In 2 of the cases, this was related to the harness—its removal resulted in recovery of the affected limb. The third case was proven to be septic arthritis, unrelated to the harness, and treatment by means of arthrotomy, intravenous antibiotics and use of the PH for hip stabilisation was used successfully. In the first two cases, the pseudoparalysis was noticed at routine review clinic, and clinical assessment, ultrasound scan and blood tests were performed. The case of septic arthritis was similarly assessed, following referral by paediatric physicians. Conclusion Pseudoparalysis in the PH is a rare event and requires careful investigation.
Prevalence and distribution of mastitis pathogens in subclinically infected dairy cows in Flanders, Belgium
Authors: Piepers Sofie , De Meulemeester Luc , de Kruif Aart , Opsomer Geert , Barkema Herman W , De Vliegher Sarne 
Published: October 2007

The main objective was to determine the prevalence of intramammary infections (IMI) in dairy cows in Flanders, Belgium. Data were obtained from quarter milk samples of dairy herds subjected to a mandatory yearly screening of all lactating cows. A total of 178 668 quarter milk samples were collected at 1087 cross-sectional dairy herd screenings performed in three consecutive years. Of the dairy cows, 40% had at least one culture-positive quarter. More than 50% of all IMI were caused by non-aureusstaphylococci.Streptococcus agalactiaeis almost eradicated in Flanders, whereasStaphylococcus aureuswas isolated from 18% of the culture-positive quarters. In addition, the distribution of mastitis pathogens in quarter milk samples from selected dairy cows with an elevated somatic cell count (SCC) is described. From 6390 cows with a geometric mean composite SCC ⩾250 000 cells/ml, nearly 65% had at least one culture-positive quarter. The majority of the IMI were caused by non-aureusstaphylococci (41·1%), whereasStaph. aureusand aesculin-positive cocci were found in respectively 25% and 18% of the culture-positive milk samples. We conclude that more efforts are needed in the prevention and control of subclinical mastitis in Flanders. Non-aureusstaphylococci are the predominant cause of IMI, warranting more research regarding the epidemiology and pathogenicity of those species.
Hypopharyngeal cancer treatment based on definitive radiotherapy: who is suitable for laryngeal preservation?
Authors: Chen S-W , Tsai M-H , Yang S-N , Liang J-A , Shiau A-C , Lin F-J 
Published: October 2007

AbstractAims:To investigate prognostic factors for survival and locoregional control in patients with stage I–IVA hypopharyngeal cancer treated with laryngeal preservation radiotherapy.Methods:This study was a retrospective analysis of 108 patients with stage I–IVA squamous cell carcinoma of the hypopharynx, treated with laryngeal preservation radiotherapy. Actuarial survival, disease-specific survival and local relapse-free survival were calculated, and multivariate analyses were performed using Cox's proportional hazards model.Results:After a median follow-up duration of 39 months, the five-year local relapse-free survival rate was 35 per cent for all patients, 66 per cent for those with stage I–II disease, 46 per cent for those with stage III disease and 20 per cent for those with stage IVA disease (p = 0.004). Multivariate analyses showed that tumour and node stages were independent prognostic factors.Conclusions:Patients with stage I–II disease were suitable for laryngeal preservation radiotherapy. For most patients with stage III–IVA disease, other than those who were T1 N1 or T2 N1, the treatment results were poor.
Advances in management of paranasal sinus aspergillosis
Authors: Daudia A , Jones N S 
Published: October 2007

AbstractSurgery remains the treatment of choice for mycetoma of the paranasal sinuses. Itraconazole has a useful role in reducing both the amount of surgery required and the amount of peri-operative bleeding in allergic aspergillosis, and continuing its use post-operatively for six weeks appears to reduce the recurrence rate (although a case–control study is required to validate this observation). In chronic invasive aspergillosis, itraconazole alone appears to be curative, although liver function tests should be monitored and other interactions considered. Imaging is required to monitor resolution; remineralisation occurs after approximately six months. In fulminant aspergillosis, radical surgery and amphotericin B continue to be the treatments of choice. This review discusses the management of aspergillosis of the paranasal sinuses, and in particular the role of itraconazole antifungal therapy.
Auricular syncope
Authors: Thakar A , Deepak K K , Shyam Kumar S 
Published: October 2007

AbstractObjective:To describe a previously unreported syndrome of recurrent syncopal attacks provoked by light stimulation of the external auditory canal.Case report:A 13-year-old girl had been receiving treatment for presumed absence seizures, with inadequate treatment response. Imaging was normal. Careful history taking indicated that the recurrent syncopal attacks were precipitated by external auditory canal stimulation. Targeted autonomic function tests confirmed a hyperactive vagal response, with documented significant bradycardia and lightheadedness, provoked by mild stimulation of the posterior wall of the left external auditory canal. Abstinence from ear scratching led to complete alleviation of symptoms without any pharmacological treatment.Conclusions:Reflex syncope consequent to stimulation of the auricular branch of the vagus nerve is proposed as the pathophysiological mechanism for this previously undocumented syndrome.
Investigation of age, sex and menstrual stage variation in human cerumen lipid composition by high performance thin layer chromatography
Authors: Koçer M , Güldür T , Akarçay M , Miman M C , Beker G 
Published: October 2007

AbstractObjective:The objective of the study was to correlate quantitative changes in the lipid composition of human cerumen with changes in age, sex and menstrual cycle stage.Design:Cerumen samples were collected from the external ear canal and analysed using sequential, one dimensional, high performance thin layer chromatography.Subjects:The following age groups of both sexes were investigated: one to 10 years; 11–18 years; 19–40 years; and 40 years and over. Additionally, cerumen samples from subjects in three stages of the menstrual cycle were compared.Results:In the cerumen samples, the peak values for wax ester and cholesterol occured between the ages of one and 10 years for both sexes. However, squalene and triglyceride content reached maximum levels at puberty. Men aged 19–40 years had a significantly greater percentage of cerumen lipid squalene content than women from the same age group; however, their cholesterol content was found to be lower. Regarding the various menstrual cycle stages, cerumen samples taken at the follicular stage from women aged 19–40 years had a significantly lower free fatty acids content, and higher cholesterol and squalene levels, compared with samples taken in the luteal or menstrual stages.Conclusion:The proportions of the lipid constituents of cerumen varied with age, sex and menstrual stage. In cerumen, the main lipid constituent stimulated at puberty appears to be squalene, not wax esters as reported for sebum. The relevance of lipid constituents to cerumen's protective role is discussed.
Revisiting the risks involved in using homograft ossicles in otological surgery
Authors: Lubbe D , Fagan J J 
Published: October 2007

AbstractDespite the fact that cartilage, cortical bone and corneal homograft tissue is still widely used in South Africa and that there has never been a reported case of human immunodeficiency virus or Creutzfeldt–Jakob disease infection transmission via this route, otolaryngologists are still reluctant to use homograft ossicles. In third world countries, such as South Africa, where ossicular prostheses are not always readily available due to financial constraints, the question arises as to whether we should be reconsidering the use of homograft ossicles. This review examines the risk of developing Creutzfeldt–Jakob disease or acquiring human immunodeficiency virus following the use of homograft ossicles during ossicular reconstruction, and discusses sterilization techniques that have proven effective in eradicating the human immunodeficiency virus and prions.For decades, homograft ossicles have been used worldwide in otological surgery, especially in patients lacking suitable autograft ossicles. There has never been a reported case of transmission of the human immunodeficiency virus, and no cases of transmissible spongiform encephalopathy have been reported in the literature after otological surgery involving only the use of homograft ossicles. There have only been two documented otological cases of Creutzfeldt–Jakob disease; these involved the use of cadaveric dura mater and pericardium for tympanic membrane grafting. The human immunodeficiency virus is easily inactivated by simple sterilisation techniques, and there is a statistically insignificant risk of transmitting this virus if proper sterilisation protocols are followed.
Foreign body reaction mimicking a second primary tumour of the oesophagus
Authors: Pollock J , Beasley N 
Published: October 2007

AbstractObjective:To present a significant complication of pharyngolaryngeal reconstruction, which resulted in diagnostic confusion as it mimicked a second primary tumour of the head and neck.Methods:Case report and review of the world literature.Case report:A 61-year-old man developed a granulomatous foreign body reaction, mimicking a second primary tumour, at the distal end of a salivary bypass tube, following pharyngolaryngectomy and reconstruction using a tubed anterolateral thigh flap. Clinically and radiologically, this was felt to represent a second primary tumour of the oesophagus, but biopsies revealed full thickness inflammation of the mucosa and granulation tissue. Repeat oesophagoscopy two weeks after removal of the tube showed complete resolution.Conclusion:No record of an oesophageal foreign body reaction to a Montgomery salivary bypass tube has previously been reported in the world literature. This report highlights the potential for such a lesion to cause diagnostic confusion with a second primary tumour.
Ear packing after ear surgery: is it really necessary?
Authors: Borgstein J , de Zwart G , Bruce I A 
Published: October 2007

AbstractObjective:We question the need for packing of the ear canal after ear surgery. For several years, it had not been the first author's standard practice to use post-operative ear packing. During this period, few problems or complications had been encountered.Setting:Tertiary referral, academic, paediatric hospital.Materials and methods:A retrospective review of all children who had undergone major ear surgery in our unit over the last year was carried out. These cases represented a full range of otological procedures. Post-operative complications and infections in the first six post-operative weeks were recorded.Results:A total of 135 ears were operated upon in 107 patients ranging in age from 11 months to 19 years (mean 9.5 years). During this time period, eight children (7.5 per cent) developed a post-operative ear infection. No cases of tympanic or meatal granulations, problems with the tympanomeatal flap, or meatal stenosis were encountered. All infections were successfully managed with topical antibiotics.Discussion:We conclude that packing after ear surgery may be safely abandoned. This would not only save valuable operating time, but would also obviate the need for pack removal, always a source of discomfort and anxiety. This is especially important in children, who may subsequently require a further general anaesthesia in order to remove the pack.
Widespread infection of the Eastern red-spotted newt (Notophthalmus viridescens) by a new species of Amphibiocystidium, a genus of fungus-like mesomycetozoan parasites not previously reported in North America
Authors: RAFFEL T. R. , BOMMARITO T. , BARRY D. S. , WITIAK S. M. , SHACKELTON L. A. 
Published: October 2007

SUMMARYGiven the worldwide decline of amphibian populations due to emerging infectious diseases, it is imperative that we identify and address the causative agents. Many of the pathogens recently implicated in amphibian mortality and morbidity have been fungal or members of a poorly understood group of fungus-like protists, the mesomycetozoans. One mesomycetozoan, Amphibiocystidium ranae, is known to infect several European amphibian species and was associated with a recent decline of frogs in Italy. Here we present the first report of an Amphibiocystidium sp. in a North American amphibian, the Eastern red-spotted newt (Notophthalmus viridescens), and characterize it as the new species A. viridescens in the order Dermocystida based on morphological, geographical and phylogenetic evidence. We also describe the widespread and seasonal distribution of this parasite in red-spotted newt populations and provide evidence of mortality due to infection.
Gene expression profiling ofLeishmania (Leishmania) donovani: overcoming technical variation and exploiting biological variation
Authors: DECUYPERE S. , VANAERSCHOT M. , RIJAL S. , YARDLEY V. , MAES L. , DE DONCKER S. , CHAPPUIS F. , DUJARDIN J.-C. 
Published: October 2007

SUMMARYGene expression profiling is increasingly used in the field of infectious diseases for characterization of host, pathogen and the nature of their interaction. The purpose of this study was to develop a robust, standardized method for comparative expression profiling and molecular characterization ofLeishmania donovaniclinical isolates. The limitations and possibilities associated with expression profiling in intracellular amastigotes and promastigotes were assessed through a series of comparative experiments in which technical and biological parameters were scrutinized. On a technical level, our results show that it is essential to use parasite harvesting procedures that involve minimal disturbance of the parasite's environment in order to ‘freeze’ gene expression levels instantly; this is particularly a delicate task for intracellular amastigotes and for specific ‘sensory’ genes. On the biological level, we demonstrate that gene expression levels fluctuate duringin vitrodevelopment of both intracellular amastigotes and promastigotes. We chose to use expression-curves rather than single, specific, time-point measurements to capture this biological variation. Intracellular amastigote protocols need further refinement, but we describe a first generation tool for high-throughput comparative molecular characterization of patients' isolates, based on the changing expression profiles of promastigotes duringin vitrodifferentiation.
Expression ofPlasmodium falciparum3D7 STEVOR proteins for evaluation of antibody responses following malaria infections in naïve infants
Authors: SCHREIBER N. , KHATTAB A. , PETTER M. , MARKS F. , ADJEI S. , KOBBE R. , MAY J. , KLINKERT M.-Q. 
Published: October 2007

SUMMARYClinical immunity toPlasmodium falciparummalaria develops after repeated exposure to the parasite. At least 2P. falciparumvariant antigens encoded by multicopy gene families (varandrif) are targets of this adaptive antibody-mediated immunity. A third multigene family of variant antigens comprises thestevorgenes. Here, 4 differentstevorsequences were selected for cloning and expression inEscherichia coliand His6–tagged fusion proteins were used for assessing the development of immunity. In a cross-sectional analysis of clinically immune adults living in a malaria endemic area in Ghana, high levels of anti-STEVOR IgG antibody titres were determined in ELISA. A cross-sectional study of 90 nine-month-old Ghanaian infants using 1 recombinant STEVOR showed that the antibody responses correlated positively with the number of parasitaemia episodes. In a longitudinal investigation of 17 immunologically naïve 9-month-old infants, 3 different patterns of anti-STEVOR antibody responses could be distinguished (high, transient and low). Children with high anti-STEVOR-antibody levels exhibited an elevated risk for developing parasitaemia episodes. Overall, a protective effect could not be attributed to antibodies against the STEVOR proteins chosen for the study presented here.
Folding of a universal ribozyme: the ribonuclease P RNA
Authors: Baird Nathan J. , Fang Xing-Wang , Srividya Narayanan , Pan Tao , Sosnick Tobin R. 
Published: October 2007

AbstractRibonuclease P is among the first ribozymes discovered, and is the only ubiquitously occurring ribozyme besides the ribosome. The bacterial RNase P RNA is catalytically active without its protein subunit and has been studied for over two decades as a model system for RNA catalysis, structure and folding. This review focuses on the thermodynamic, kinetic and structural frameworks derived from the folding studies of bacterial RNase P RNA.
Linear stability analysis of the figure-eight orbit in the three-body problem
Authors: ROBERTS GARETH E. 
Published: October 2007

AbstractWe show that the well-known figure-eight orbit of the three-body problem is linearly stable. Building on the strong amount of symmetry present, the monodromy matrix for the figure-eight is factored so that its stability can be determined from the first twelfth of the orbit. Using a clever change of coordinates, the problem is then reduced to a 2×2 matrix whose entries depend on solutions of the associated linear differential system. These entries are estimated rigorously using only a few steps of a Runge–Kutta–Fehlberg algorithm. From this, we conclude that the characteristic multipliers are distinct and lie on the unit circle. The methods and results presented are applicable to a wide range of Hamiltonian systems containing symmetric periodic solutions.
Non-standard smooth realizations of Liouville rotations
Authors: FAYAD B. R. , SAPRYKINA M. , WINDSOR A. 
Published: October 2007

AbstractWe augment the $C^\infty $ conjugation approximation method with explicit estimates on the conjugacy map. This allows us to construct ergodic volume-preserving diffeomorphisms measure-theoretically isomorphic to any a priori given Liouville rotation on a variety of manifolds. In the special case of tori the maps can be made uniquely ergodic.