bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evidence for local synthesis of antibodies to denatured collagen in the synovium in rheumatoid arthritis
Authors: Rowley Merrill J. , Williamson D.James , Mackay Ian R. 
Published: February 2007

AbstractSynovial fluid samples from 36 patients with rheumatoid arthritis (RA) and 31 patients with other articular diseases (OAD) were examined for the presence of antibodies to denatured or native human type II collagen. Levels of IgG antibodies to denatured or native human type II collagen, rheumatoid factor, immunoglobulins, and total proteins were assessed in paired samples of serum and synovial fluid from 21 patients with RA and from 14 patients with OAD. Solid‐phase radioimmunoassay showed that levels of antibodies to denatured collagen in synovial fluid were significantly higher in RA patients than in OAD patients (median 3,270, range 44‐‐16,816 versus median 919, range 119—5,814; P < 0.001). These antibody levels were higher in synovial fluid than in the serum of RA patients, but not in patients with OAD. Paired serum and synovial fluid samples showed no correlation between the level of antibodies to denatured collagen and levels of either IgG, IgA, IgM, or rheumatoid factor. Synovial fluid antibodies to native collagen were higher in RA patients. Antibodies to collagen may be synthesized preferentially in synovial tissues and, hence, participate in the perpetuation of RA.
Clonal proliferation of large granular lymphocytes in rheumatoid arthritis
Authors: Loughran Thomas P. , Starkebaum Gordon , Kidd Pamela , Neiman Paul 
Published: February 2007

AbstractSome patients with chronically elevated large granular lymphocyte (LGL) numbers have rheumatoid arthritis (RA). Since these patients also may have neutropenia and splenomegaly, their symptoms resemble those of patients diagnosed as having Felty's syndrome (FS). We studied the immunophenotypic and genotypic characteristics of mononuclear cells from patients with RA and neutropenia to better determine the extent of heterogeneity in this condition. Four patients had markedly increased numbers of LGLs, which expressed HNK‐1 antigen and IgG Fc receptors. In contrast, the remaining 8 patients, who had FS, had normal LGL counts, and surface marker studies showed normal numbers of HNK‐1 and IgG Fc receptor positive cells. Clonal rearrangement of the T cell receptor β chain gene was demonstrated in all 4 patients with excess LGLs, whereas a germline configuration of this gene was present in all 6 FS patients in whom this was studied. These results suggest that there are diverse groups among patients with RA and neutropenia. Since prognosis may differ, it is important to recognize that some patients who are considered to have Felty's syndrome may have a clonal proliferation of LGLs.
B cell hyperactivity and its relation to distinct clinical features and the degree of disease activity in patients with systemic lupus erythematosus
Authors: Sakane Tsuyoshi , Suzuki Noboru , Takada Shinsuke , Ueda Yuji , Murakawa Yohko , Tsuchida Tomiko , Yamauchi Yasutaka , Kishimoto Tadamitsu 
Published: February 2007

AbstractPeripheral blood B cells that were actively proliferating, those actively secreting immunoglobulin, and those expressing an early activation marker, Ba antigens, on the surfaces were quantitated in 25 patients with systemic lupus erythematosus (SLE). B cell hyper‐activity was found in almost all of the SLE patients, as demonstrated by any one of these measures of B cell activity. Moreover, we observed a strong positive correlation between the degree of disease activity and the amount of spontaneous incorporation of 3H‐thymidine by B cells; the magnitude of the increase in frequency of spontaneous Ig‐secreting cells in peripheral blood correlated strikingly with certain clinical features in these patients. Our findings suggest that there is heterogeneity of B cell hyperactivity in individual patients with SLE and, thus, that clinical subsets of SLE can be identified on the basis of B cell hyperactivity.
Impaired mobility of human t lymphocyte surface molecules during inactive systemic lupus erythematosus. relationship to a defective camp pathway
Authors: Kammer Gary M. , Mitchell Elizabeth 
Published: February 2007

AbstractThe T lymphocytes of patients with active systemic lupus erythematosus (SLE) exhibit impaired capping of the surface molecules CD3, CD4, and CD8 and a defective cAMP‐dependent pathway. Since the mobility of these molecules is regulated in part by cAMP, we sought to determine whether there is a specific defect(s) along the T cell cAMP pathway that contributes to the persistent capping disorder observed during inactive SLE. The data suggest that a defect may exist at the level of cAMP‐dependent protein kinase activation or at a point distally. We propose that a disorder of cAMP‐dependent protein kinase activity might account for the defect of capping observed in both the CD3, CD4 (helper/inducer) and CD3,CD8 (suppressor) subsets observed in SLE.
Nailfold capillary microscopy in polymyositis and dermatomyositis
Authors: Ganczarczyk M. Lynn , Lee Peter , Armstrong Sara K. 
Published: February 2007

AbstractNailfold capillary microscopy was performed in 19 patients with polymyositis (PM) and 16 with dermatomyositis (DM). Enlarged capillary loops were seen more frequently in patients with DM (56%) than in those with PM (21%). Avascular lesions occurred in both PM and DM, but were more prevalent and more severe in patients with DM. Raynaud's phenomenon, arthritis, and pulmonary involvement were associated with greater numbers of enlarged capillary loops and more severe avascular lesions. The severity of the observed microvascular abnormalities did not correlate with the occurrence of malignancy or active myositis, but appeared to lessen with prolonged disease remission.
Hla and immunoglobulin allotypes in mixed connective tissue disease
Authors: Black C. M. , Maddison P. J. , Welsh K. I. , Bernstein R. , Woodrow J. C. , Pereira R. S. 
Published: February 2007

AbstractA group of patients with mixed connective tissue disease (MCTD) were HLA and immunoglobulin allotyped. We found that the incidence of DR4 in the patient group was increased compared with that in the normal controls, but the increase was restricted to the subgroup of patients with arthritis. The age at onset of MCTD was lower in patients with DR4 and higher in patients with DR2 compared with patients who did not have these antigens. A1, B8, and DR3 were more frequent, but not significantly so, in the MCTD patient group. We also found that there was a significant perturbation of the Gm allotype frequencies in patients with MCTD.
The terminal complement complex, c5b–9, a marker of disease activity in patients with systemic lupus erythematosus
Authors: Gawryl Maria S. , Chudwin David S. , Langlois Paul F. , Lint Thomas F. 
Published: February 2007

AbstractConcentrations of the terminal complement complex (TCC), C5b–9, were examined in 120 serum samples from 28 patients with systemic lupus erythematosus. Eleven patients with various manifestations of the disease were followed longitudinally for a 2‐year period during active and inactive phases of the disease. In 9 of the 11 patients, elevations in TCC concentrations correlated with disease exacerbations. In many of these patients, C3 and C4 levels remained normal during the study and were less sensitive indicators of disease activity than were TCC concentrations. We believe that measurements of TCC are useful in monitoring patients with rheumatic diseases in which complement activation is a component.
Stimulation of rabbit synoviocyte prostaglandin e2 SYNTHESIS BY LIPOPOLYSACCHARIDES AND THEIR SUBUNIT STRUCTURES
Authors: Rothenberg Ralph J. , England Douglas , Qureshi Nilofer , Takayama Kuni 
Published: February 2007

AbstractLipopolysaccharides (LPS) induce synoviocyte activation and may lead to destruction of synovial joint tissues. We assessed the production of prostaglandin E2 (PGE2) as a measure of synoviocyte activation by LPS and their subunit structures. Diphosphoryl lipid A was the smallest portion of lipid A tested that stimulated PGE2 production. The polysaccharide fraction of LPS, containing the O antigen, was also active. Intraarticular injections of the polysaccharide resulted in a synovitis very similar to that found in association with the intact LPS molecule. These observations suggest that both parts of LPS might be involved in gram‐negative, organism‐associated synovitis.
Human parvovirus b19 dna in synovial fluid
Authors: Dijkmans B. A. C. , Van Elsacker‐Niele A. M. W. , Salimans M. M. M. , Van Albada‐Kuipers G. A. , De Vries E. , Weiland H. T. 
Published: February 2007

AbstractWe describe a 33‐year‐old woman with a serologically proven human parvovirus B19 infection, who developed synovitis. Using a dot‐blot hybridization technique, we detected B19 DNA in her synovial fluid. To our knowledge, this is the first report of the isolation of B19 from synovial fluid.
Synovial complications of spondylepiphyseal dysplasia of late onset
Authors: Sambrook P. N. , De Jager J. P. , Champion G. D. , Doust B. D. , Mcgirr E. E. , Kozlowski K. S. , Sillence D. O. 
Published: February 2007

AbstractWe describe 2 patients with a late‐onset type of spondylepiphyseal dysplasia who developed multiple synovial complications. The synovial manifestations in the first patient included recurrent episodes of acute arthritis due to calcium pyrophosphate dihydrate crystal deposition and, later, chronic synovitis with radiologically evident chondrocalcinosis. In the second patient, cholesterol crystals in synovial fluid, multiple osteochondromata, and a histologic appearance resembling pigmented villonodular synovitis on synovial biopsy were seen. Osteochondromata were identified in 4 other members of this patient's family. Synovial manifestations in the osteochondrodysplasias may be more common than previously recognized.
Arthritis‐associated changes in flow cytometric characteristics of cultured synovial fibroblasts
Authors: Eerola Erkki , Pulkki Kari , Pelliniemi Lauri J. , Granfors Kaisa , Vuorio Eero , Toivanen Auli 
Published: February 2007

AbstractSynovial fibroblasts cultured from patients with rheumatoid or reactive arthritis and from controls were studied by flow cytometry, spectroscopy, and electron microscopy. Analysis of 29 cell lines revealed consistent differences between arthritic and normal fibroblasts. Cells cultured from inflamed synovial tissue exhibited higher autofluorescence than did control fibroblasts, and displayed exceptional light scatter properties in flow cytometry, indicating changes in cytoplasmic structures. Electron microscopic examination of the fibroblasts from arthritic synovial tissue revealed large numbers of round, swollen, laminated, mitochondrion‐like bodies, which were not observed in the control fibroblasts. The changes observed by flow cytometry (light scatter and autofluorescence) coincided with the presence of the mitochondrion‐like organelles. The strong autofluorescence observed in the arthritic fibroblasts resembled the fluorescence spectrum of mitochondrial flavoproteins. These data suggest that persistent metabolic and structural changes have occurred in the mitochondria of synovial fibroblasts and inflammatory synovial tissue. The usefulness of flow cytometry in identifying such cells is described.
Autoantibodies to mitochondria in systemic sclerosis. frequency and characterization using recombinant cloned autoantigen
Authors: Fregeau David R. , Leung Patrick S. C. , Coppel Ross L. , Mcneilage L. Jane , Medsger Thomas A. , Gershwin M. Eric 
Published: February 2007

AbstractMitochondrial autoantibodies, a hallmark of primary biliary cirrhosis (PBC), have been widely described for many years in patients with systemic sclerosis, and there have been several reports of the concurrence of systemic sclerosis and PBC. However, there is very little information with respect to the significance of these autoantibodies or any definitive evidence that the antigens involved represent the mitochondrial autoantigens (M2 complex) described in PBC. We have cloned and sequenced a rat complementary DNA which encodes for all the epitopes recognized by autoantibodies to the major, or 70‐kd, mitochondrial autoantigen in patients with PBC. Using this recombinant fused autoantigen, as well as by immunoblotting with human placental mitochondria, we tested for antimitochondrial antibody specificity in sera from 250 patients with systemic sclerosis. Nineteen sera (7.6%), including those from patients with CREST (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasias) and diffuse scleroderma, had reactivity with human placental mitochondria proteins by immunoblot testing. All 19 sera reacted with the M2 complex. All sera that reacted with the 70‐kd protein likewise reacted with the recombinant cloned autoantigen. The predominant autoantibody isotype to the 70‐kd protein was IgG3. Interestingly, the 70‐kd protein is 11% proline, an amino acid which is frequently preceded by hydrophobic amino acids.
Enhanced production of interleukin‐2 in patients with progressive systemic sclerosis. hyperactivity of cd4‐positive t cells?
Authors: Umehara Hisanori , Kumagai Shunichi , Ishida Hiroshi , Suginoshita Toshihiko , Maeda Michiyuki , Imura Hiroo 
Published: February 2007

AbstractA significant enhancement of interleukin‐2 production was observed in phytohemagglutinin‐stimulated peripheral lymphocytes from patients with progressive systemic sclerosis (PSS). Their Leu‐3a:Leu‐2a ratio was also significantly higher than normal. Moreover, Leu‐3a+ cells freshly purified from PSS patients' blood produced much more interleukin‐2 than did cells from normal subjects. These results suggest that T cell hyperactivity, especially among Leu‐3a+ T cells, occurs in patients with PSS.
Encapsulation of indomethacin in liposomes provides protection against both gastric and intestinal ulceration when orally administered to rats
Authors: Soehngen Ellen C. , Godin‐Ostro Evelyn , Fielder Francis G. , Ginsberg Richard S. , Slusher Michael A. , Weiner Alan L. 
Published: February 2007

AbstractEncapsulation of indomethacin into egg phospha‐tidylcholine (EPC) monophasic vesicles (MPV) or into stable plurilamellar vesicles (SPLV) before oral administration to rats substantially reduced or eliminated the gastric and intestinal ulceration normally associated with ingestion of this drug. Ulcers were assessed by the 4‐hour single‐dose gastric ulceration model and the 4‐ or 14‐day repeated‐dose intestinal ulceration model, using microscopic/planimetric quantitation. Oral dosages of up to 10 mg/kg of indomethacin in polyethylene glycol‐400 resulted in substantial gastric ulceration, but not when given in methylcellulose suspension or as EPCMPV. Severe intestinal ulcers resulted following oral administration of indomethacin in either vehicle at daily 3–4‐mg/kg doses, but did not result from EPCMPV formulations, whether dosed for 4 days or 14 days. Oral administration of pH‐sensitive indomethacin liposomes constructed from cholesterol hemisuccinate resulted in loss of the protective action. Indomethacin‐MPV showed both comparable bioactivity and comparable blood levels of the drug when contrasted with free drug in vehicles. Biodistribution studies demonstrated that when delivered from liposomes, drug and phospho‐lipid are rapidly cleared through the stomach but then are differentially absorbed. Empty EPCMPV given by mouth also offered some protection against ulcers induced by systemic (subcutaneous) introduction of indomethacin, although better protective action was noted when the drug was first liposome‐encapsulated and then given orally. The application of liposomes to the development of nonsteroidal antiinflammatory drugs that have minimal gastrointestinal side effects is discussed.
Some adverse reactions to allopurinol may be mediated by lymphocyte reactivity to oxypurinol
Authors: Emmerson B. T. , Hazelton R. A. , Frazer I. H. 
Published: February 2007

AbstractSix of 9 patients with previously documented adverse reactions to allopurinol exhibited transformation of their peripheral blood lymphocytes when these were exposed in vitro to the allopurinol metabolite, oxypurinol. In 2 of these subjects, intradermal skin challenge was positive at 48 hours with either allopurinol or oxypurinol. The evidence presented suggests that some adverse reactions to allopurinol represent delayed‐type hypersensitivity to oxypurinol.
Augmented interleukin‐1 production and hla–dr expression in the synovium of rheumatoid arthritis patients
Authors: Miyasaka Nobuyuki , Sato Kazuto , Goto Makoto , Sasano Minoru , Natsuyama Motonobu , Inoue Kazuhiko , Nishioka Kusuki 
Published: February 2007

AbstractPotent interleukin‐1 (IL‐1) activity was detected in culture supernatants from synovium, obtained by arthroscopy, from rheumatoid arthritis (RA) patients but not from non‐RA patients. Production of IL‐1 by RA synovium correlated well with findings of inflammation on arthroscopy and HLA–DR expression in immunohistochemical staining. Furthermore, there was a positive correlation between IL‐1 production from RA synovium and joint changes detected on roentgenograms. These findings strongly suggest that IL‐1 might play an important role in the joint destruction in RA.
Quantitative immunoassay of anti‐la antibodies using purified recombinant la antigen
Authors: T. Clair E. William S , Pisetsky David S. , Reich Charles F. , Chambers Jasemine C. , Keene Jack D. 
Published: February 2007

AbstractA purified recombinant La fusion protein was tested in an enzyme‐linked immunosorbent assay to quantitate anti‐La responses. This protein contained the immunodominant region of the La molecule fused to β‐galactosidase. In solid‐phase assays, recombinant La protein was solubilized in urea and bound to polystyrene wells without loss of immunoreactivity. The recombinant‐based enzyme‐linked immunosorbent assay proved to be a sensitive method for the detection of anti‐La binding, and it accurately distinguished anti‐La precipitin positive sera from normal sera.
Ten‐year followup study of patients with yersinia arthritis
Authors: Leirisalo‐Repo Marjatta , Suoranta Hannu 
Published: February 2007

AbstractEighty‐five patients with acute Yersinia arthritis were seen in followup for a mean of 10 years. During that time, peripheral joint symptoms occurred frequently (51.8%), but these symptoms were mild (45.9%). Development of a new reactive arthritis (4.7%) or chronic arthritis (2.4%) was uncommon. One‐third of the patients experienced low back pain, and one‐third of the patients had radiologic evidence of sacroiliitis. The presence of sacroiliitis was more frequent in patients with low back pain (46.7%) than in those who did not have symptoms (21.2%). More patients with HLA–B27 had low back pain and sacroiliitis, but there was no association of this genetic factor with the residual symptoms in peripheral joints.
Pain management in rheumatoid arthritis patients
Authors: Parker Jerry C. , Frank Robert G. , Beck Niels C. , Smarr Karen L. , Buescher Keith L. , Phillips Les R. , Smith Elaine I. , Anderson Sharon K. , Walker Sara E. 
Published: February 2007

AbstractTo examine the effectiveness of a cognitive‐behavioral pain management program for patients with rheumatoid arthritis, three patient groups were studied: a cognitive‐behavioral group (CB), an attention‐placebo group, and a control group. The CB group received a comprehensive, 12‐month pain management program that taught coping strategies such as problem‐solving techniques, relaxation training, strategies for attention diversion, and training in family dynamics and communication. Dependent measures included pain, coping strategies, psychological status, functional status, and disease status. Data analysis at 12 months revealed benefits for the CB group in the area of enhanced coping strategies. Specifically, the CB subjects showed significantly greater use of coping strategies and significantly more confidence in their ability to manage pain. The findings are discussed in terms of the importance of enhanced self‐efficacy and personal control for patients with rheumatoid arthritis.
Reduced mandibular dimensions and asymmetry in juvenile rheumatoid arthritis
Authors: Stabrun Anne E. , Larheim Tore A. , Höyeraal Hans M. , Rösler Marek 
Published: February 2007

AbstractThe pathogenesis of reduced mandibular dimensions and asymmetry was studied in 103 patients with juvenile rheumatoid arthritis. Abnormalities of the temporomandibular joint were intimately related to mandibular growth disturbances. Unilateral joint abnormality resulted in jaw asymmetry and underdevelopment on the affected side. Duration of disease and of corticosteroid therapy, age at disease onset, and age when the data were recorded were correlated with the size of the mandible. Our findings did not support a hypothesis of cervical spine involvement as an etiologic factor of reduced mandibular size.
Increased levels of plasma anaphylatoxins in systemic lupus erythematosus predict flares of the disease and may elicit vascular injury in lupus cerebritis
Authors: Hopkins Patricia , Michael Belmont H. , Buyon Jill , Philips Mark , Weissmann Gerald , Abramson Steven B. 
Published: February 2007

AbstractWe measured levels of complement anaphylatoxin split products, C3a and C5a, in the circulation of patients with systemic lupus erythematosus (SLE). In 23 SLE patients who were followed serially, the mean C3a value was 179 ng/ml during stable disease and 550 ng/ml during a disease flare. In 10 patients, C3a levels predicted disease activity, with the C3a value rising from a mean of 183 ng/ml at a time of stable disease to a mean of 242 ng/ml 1–2 months prior to a clinical exacerbation of disease. The mean C3a level in 5 patients with acute dysfunction of the central nervous system (CNS) was 1,297 ng/ml, which is significantly higher than that observed in patients with active disease but without CNS involvement (P < 0.01). C5a levels were also significantly elevated in 4 patients with acute CNS disease. Pathologic specimens from 2 patients who died during an acute lupus flare revealed neutrophils occluding the cerebral and intestinal vessels. Fluorescein angiography in a patient with CNS lupus revealed vasoocclusive retinopathy. In 5 of 7 SLE patients who were pregnant, C3a levels were elevated, with a group mean value of 310 ng/ml. There was a negative correlation (r = – 0.59) between C3a and C3 levels in pregnant patients with SLE, and this finding is consistent with complement activation as the cause of decreasing C3 levels. We suggest that serial measurements of C3a can predict flares of disease in lupus patients and can demonstrate complement activation during pregnancy in women with SLE. In addition, release of C3a and C5a (mediators of inflammation) into the circulation may elicit vascular injury, particularly in patients with lupus cerebritis.