bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of the microbiome in Apis cerana honey highlights honey as a potential source for the isolation of beneficial bacterial strains
Authors: Lanh Pham T. , Duong Bui T.T. , Thu Ha T. , Hoa Nguyen T. , Quyen Dong Van 
Published: March 2024

Background
Honey is a nutritious food made by bees from nectar and sweet deposits of flowering plants and has been used for centuries as a natural remedy for wound healing and other bacterial infections due to its antibacterial properties. Honey contains a diverse community of bacteria, especially probiotic bacteria, that greatly affect the health of bees and their consumers. Therefore, understanding the microorganisms in honey can help to ensure the quality of honey and lead to the identification of potential probiotic bacteria.

Methods
Herein, the bacteria community in honey produced by Apis cerana was investigated by applying the next-generation sequencing (NGS) method for the V3–V4 hypervariable regions of the bacterial 16S rRNA gene. In addition, lactic acid bacteria (LAB) in the honey sample were also isolated and screened for in vitro antimicrobial activity.

Results
The results showed that the microbiota of A. cerana honey consisted of two major bacterial phyla, Firmicutes (50%; Clostridia, 48.2%) and Proteobacteria (49%; Gammaproteobacteria, 47.7%). Among the 67 identified bacterial genera, the three most predominant genera were beneficial obligate anaerobic bacteria, Lachnospiraceae (48.14%), followed by Gilliamella (26.80%), and Enterobacter (10.16%). Remarkably, among the identified LAB, Lactobacillus kunkeei was found to be the most abundant species. Interestingly, the isolated L. kunkeei strains exhibited antimicrobial activity against some pathogenic bacteria in honeybees, including Klebsiella spp., Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus. This underscores the potential candidacy of L. kunkeei for developing probiotics for medical use. Taken together, our results provided new insights into the microbiota community in the A. cerana honey in Hanoi, Vietnam, highlighting evidence that honey can be an unexplored source for isolating bacterial strains with potential probiotic applications in honeybees and humans.
Commercial Culture of Cordyceps militaris from Waste Products of Sericulture in India
Authors: Narzary P. R. , Brahma D. , Sarma J. , Nath I. , Dutta P. L. , Bora N. R. 
Published: March 2024

Cordyceps militaris, an entomopathogenic fungi belonging to Ascomycota group are currently in the limelight for its synthesis of Cordycepin, – an anti-cancer component. The host range of Cordyceps is extremely broad, with predominance on the orders Lepidoptera, Coleoptera, and Hymenoptera. Although this fungus is extremely rare in nature, its artificial culture is becoming more and more popular on the global market. However, the commercial culture of this fungus has been hampered by the difficulty in getting seeding material and the complex developmental technique. For ages, the silkworm (Order: Lepidoptera) has played a significant economic role in the food and silk industries of Asian nations. The waste product of the silkworm rearing process is the dead silkworm pupae, which are generated in large quantities after stifling of the cocoons for the purpose of producing raw silk. Waste from Sericulture in India has the potential to be used as a promising culture media for the growth of the Cordyceps-related sector, helping the silk farmers in their quest for future financial gain and the social advancement of the tribal society.
End-to-Side Neurorrhaphy Technique: Exploring Neuronal Regeneration to Restore Nerve Function
Authors: Oliveira Raiza Rafaela Borges de , Maia Júlia Santos , Galvão Ismael Felipe Gonçalves , Leite Davi Rodrigues , Azevedo-Filho Hildo Rocha Cirne , Souza Fernando Henrique Morais de 
Published: March 2024

Aims: To report the case of a pediatric patient with total left brachial plexopathy (TLBP), exhibiting partially restored motor function and impaired sensitivity, who was treated with the End-to-Side neurorrhaphy (ETSN) technique.
Presentation of Case: A 13-year-old female was involved in a car accident. After clinical and imaging evaluation, the diagnostic hypothesis was TLBP with partially restored function and loss of protective sensitivity in the pincer region. A surgical intervention was recommended. Due to case characteristics, ETSN appeared to be the most appropriate technique.
Discussion: ETSN is a technique utilized for repairing nerve injuries. It involves suturing the distal end of a transected nerve to the lateral side of a healthy donor nerve, aiming to restore neurons through peripheral plasticity. The regeneration mechanism promoted by this technique shares some similarities with that promoted by standard techniques, with important roles being assigned to Schwann cells (SC), for example. However, this procedure has its own particularities, which still require further investigation. Generally, ETSN cannot replicate the efficacy of model techniques, but remains valid in select cases, such as instances involving nerve injuries exceeding 3-4 cm or in the absence of donor nerves. Therefore, ETSN stands as a choice and deserves consideration.
Conclusion: The patient progressed with a good prognosis and recovered protective sensitivity in the pincer region. ETSN is efficient for peripheral nerve injuries with inaccessible proximal trunks or long nerve defects. The discussion surrounding ETSN remains controversial, making this study essential for expanding scientific knowledge.
An approximate analytical solution of transport model in electrodes for anode‐supported solid oxide fuel cells
Authors: Bao Cheng , Cai Ningsheng 
Published: October 2007

AbstractOn the basis of the one‐dimensional, isothermal mechanistic model in the anode of an anode‐supported solid oxide fuel cell using binary mixture as fuel, two approximate analytical solutions considering both mass and charge transfer were developed in this article. First, by dividing the anode into the inactive and active zones, the regular perturbation method was used to approximate the distribution of the fuel concentration and overpotential. Then the approximate solution using the singular perturbation method reflects both the boundary‐layer effects of current density at the two boundaries of the anode. Both the two approximate solutions agree well with the numeric solution in the case of medium and low electric load. By introducing the concept of the boundary‐layer thickness at the anode/electrolyte interface, the two approximate solutions can be combined to get an explicit expression in a wide range of operation. The result in this article is useful to system‐level modeling and dynamic analysis. © 2007 American Institute of Chemical Engineers AIChE J, 2007
Influence of pore structure on drying kinetics: A pore network study
Authors: Metzger Thomas , Irawan Anton , Tsotsas Evangelos 
Published: October 2007

AbstractA pore network model for convective drying is applied to investigate the influence of structure on drying behavior. The isothermal drying model is based on the work of Prat, and uses invasion percolation concepts, neglecting any viscous or gravitational effects. Pore structures of different pore‐size distribution and coordination number are studied in two‐ and three‐dimensions. Results are given as phase distributions or moisture profiles during drying, as well as drying rate curves. Significant differences in drying kinetics are reported for a variation in coordination number of monomodal networks. Bimodal pore structures can produce dramatically higher drying rates, but only for certain spatial correlations of small and large pores. Finally, influence of product thickness and importance of boundary layer modeling are addressed. © 2007 American Institute of Chemical Engineers AIChE J, 2007
Molecular characterization of HOXA13 polyalanine expansion proteins in hand–foot–genital syndrome
Authors: Utsch Boris , McCabe Colleen D. , Galbraith Kenneth , Gonzalez Ricardo , Born Mark , Dötsch Jörg , Ludwig Michael , Reutter Heiko , Innis Jeffrey W. 
Published: October 2007

AbstractWe report on a father and daughter with hand–foot–genital syndrome (HFGS) with typical skeletal and genitourinary anomalies due to a 14‐residue polyalanine expansion in HOXA13. This is the largest (32 residues) polyalanine tract so far described for any polyalanine mutant protein. Polyalanine expansion results in protein misfolding, cytoplasmic aggregation and degradation; however, HOXA13 polyalanine expansions appear to act as loss of function mutations in contrast to gain of function for HOXD13 polyalanine expansions. To address this paradox we examined the cellular consequences of polyalanine expansions on HOXA13 protein using COS cell transfection and immunocytochemistry. HOXA13 polyalanine expansion proteins form cytoplasmic aggregates, and distribution between cytoplasmic aggregates or the nucleus is polyalanine tract size‐dependent. Geldanamycin, an Hsp90 inhibitor, reduces the steady‐state abundance of all polyalanine‐expanded proteins in transfected cells. We also found that wild‐type HOXA13 or HOXD13 proteins are sequestered in HOXA13 polyalanine expansion cytoplasmic aggregates. Thus, the difference between HOXA13 polyalanine expansion loss‐of‐function and HOXD13 polyalanine expansion dominant‐negative effect is not the ability to aggregate wild‐type group 13 paralogs but perhaps to variation in activities associated with refolding, aggregation or degradation of the proteins. © 2007 Wiley‐Liss, Inc.
Posterior polymorphous corneal dystrophy is associated with TCF8 gene mutations and abdominal hernia
Authors: Aldave Anthony J. , Yellore Vivek S. , Yu Fei , Bourla Nirit , Sonmez Baris , Salem Andrew K. , Rayner Sylvia A. , Sampat Kapil M. , Krafchak Charles M. , Richards Julia E. 
Published: October 2007

AbstractMutations in the two‐handed zinc‐finger homeodomain transcription factor gene (TCF8) have been associated with posterior polymorphous corneal dystrophy (PPCD) and extraocular developmental abnormalities. We performed screening of TCF8 in 32 affected, unrelated probands, affected and unaffected family members of probands identified with a TCF8 mutation, and in 100 control individuals. Eight different pathogenic mutations were identified in eight probands: four frameshift (c.953_954insA, c.1506dupA, c.1592delA, and c.3012_3013delAG); three nonsense (Gln12X, Gln214X, Arg325X); and one missense (Met1Arg). Screening of TCF8 in affected and unaffected family members in six families demonstrated that each identified mutation segregated with the disease phenotype in each family; two probands did not have additional family members available for analysis. None of the eight TCF8 mutations was identified in 200 control chromosomes. The prevalence of hernias of the abdominal region in affected individuals with PPCD associated with TCF8 mutations was significantly higher than the prevalence in both individuals with PPCD not associated with a TCF8 mutation and in unaffected individuals. Therefore, PPCD is associated with TCF8 mutations in one quarter of affected families in this study, or about one third of all PPCD families that have been screened thus far. In these families, the presence of apparently causative TCF8 mutations is associated with abdominal and inguinal hernias. © 2007 Wiley‐Liss, Inc.
Somatic and gonadal mosaicism in X‐linked retinitis pigmentosa
Authors: Jin Zi‐Bing , Gu Feng , Matsuda Hirokazu , Yukawa Nobuhiro , Ma Xu , Nao‐i Nobuhisa 
Published: October 2007

AbstractThe g.ORF15 + 652–653delAG mutation in the RPGR gene is the most frequent mutation in X‐linked retinitis pigmentosa (XLRP). The objective of this study was to investigate the possibility of mosaicism in an XLRP family. Eight subjects in the RP family were recruited. Blood samples were collected for DNA extraction. Haplotype analysis and mutational screening on the RPGR gene were performed. Additionally, samples of hair follicles and buccal cells from the mother of the proband were acquired for DNA extraction and molecular analysis. Phenotype was characterized with routine ophthalmic examination, Goldmann perimetry, electroretinography, and color fundus photography. A g.ORF15 + 652–653delAG mutation was identified in second‐ and third‐generation patients/carriers. A first‐generation female, who was considered to be an obligate carrier, demonstrated a normal phenotype as well as a normal genotype in lymphocytic DNA, indicating the gonadal mosaicism; however, a heterozygous AG‐deletion at nucleotide 652 and 653 was identified in the genomic DNA of hair follicles, hair shaft, and buccal cells, indicating that the mutation is somatic. In conclusion, we reported on a family in which an asymptomatic woman with somatic–gonadal mosaicism for a RPGR gene mutation transmitted the mutation to an asymptomatic daughter and to a son with XLRP. Gonadal mosaicism may be responsible for a proportion of multiplex or simplex RP families, in which more than 50% of all cases of RP are found. © 2007 Wiley‐Liss, Inc.
Association study of protease activated receptor 1 gene polymorphisms and adverse pregnancy outcomes: Results of a pilot study in Israel
Authors: Grisaru‐Granovsky Sorina , Tevet Aharon , Bar‐Shavit Rachel , Salah Zaidoun , Elstein Deborah , Samueloff Arnon , Altarescu Gheona 
Published: October 2007

AbstractProtease activated receptor 1 (PAR1), the thrombin receptor, is implicated in hemostasis, tissue remodeling, and is critical in early placentation. PAR1 polymorphisms that influence coagulation and adhesion molecule expression may compromise formation of a normal placenta, thereby resulting in adverse outcomes. This study is a prospective, case‐control comparison of Israeli mother‐neonate (singletons) pairs of complicated pregnancies: 33 preterm deliveries (PTDs), 20 preeclampsia (PE), and 28 idiopathic small for gestational age neonates (SGA) versus 98 pairs of uncomplicated singleton deliveries. PCR amplification identified polymorphisms in the 5′ regulatory region of the PAR1 gene at [−1426(C/T)], insertion at [−506 (I/D)], and IVS at [−14 (A/T)]. We found that 15.2% mothers with PTD were heterozygous for [−1426] versus 3% in uncomplicated deliveries; however, maternal allele frequency was not significantly different between pregnancies complicated by PE or SGA versus uncomplicated pregnancies. Maternal allele frequencies for [−506] and [−14] polymorphisms were not significantly different between any of the study groups compared to the uncomplicated group. Haplotype analysis recapitulated the genotype pattern. Maternal homozygous allele frequency for each of the polymorphic variants was low (<2%) in all pregnancies, and equally distributed. Neonatal genotypes did not differ between groups and were not associated with adverse outcomes. Maternal heterozygous allele state for PAR1 polymorphism [−1426] and the haplotype with [−506 wild‐type]/[−1426T]/[IVS‐14A] is associated with PTD. © 2007 Wiley‐Liss, Inc.
An unclassifiable short rib‐polydactyly syndrome with acromesomelic hypomineralization and campomelia in siblings
Authors: Kannu Peter , McFarlane Jeannette H. , Savarirayan Ravi , Aftimos Salim 
Published: October 2007

AbstractThe short rib‐polydactyly (SRP) group are lethal skeletal dysplasias with an autosomal recessive inheritance pattern that can be distinguished on radiological and histological grounds. We report on two consecutive pregnancies complicated by a SRP syndrome with acromesomelic hypomineralization and campomelia that cannot be definitely categorized, yet possesses features of this group of conditions. The skeletal changes observed in both cases included markedly shortened ribs, shortened humeri and femora, limb bowing, absent ossification of the radii, ulnae, tibiae and fibulae, as well as the bony elements of the hands and feet, hypoplastic scapulae and peritoneal calcifications. In one case, the pancreas was abnormal in shape, without a tail and the spleen was not identified. Ectopic splenic tissue and intestinal malrotation were identified and were suggestive of a laterality disorder. Whether these two cases should be considered an atypical form of SRP cannot be completely resolved at this present time and will need to wait on further progress in molecular testing. © 2007 Wiley‐Liss, Inc.
Long‐term follow‐up and analysis of monozygotic twins concordant for 45,X/46,XY peripheral blood karyotype but discordant for phenotypic sex
Authors: Tho Sandra P. , Jackson Robert , Kulharya Anita S. , Reindollar Richard H. , Layman Lawrence C. , McDonough Paul G. 
Published: October 2007

AbstractWe report on the follow‐up of a set of monozygotic (MZ) twins who were concordant for peripheral blood karyotype 45,X/46,XY but discordant for phenotypic sex. One twin is a phenotypically normal male and the other twin has asymetrical gonadal dysgenesis. The female twin has the mos45,X/46,XY karyotype in all four tissues: left testis, right streak, vas deferens, and clitoral skin. The normal male twin has the normal 46,XY karyotype in all three tissues tested: foreskin, scrotal skin, and testis. Follow‐up of the twins at age 21, revealed persistence of mos45,X/46,XY karyotype in peripheral blood into adult life. However, the male grew up with normal male stature, reaching an adult height of 182 cm. The female twin received low dose estrogen replacement with complete breast development at age 14 years. She reached an adult height of 156 cm. At 21 years of age the male twin had normal testicular endocrine function, but severe oligospermia. The long‐term follow‐up of this set of MZ twins indicate that the male twin has the mosaicism confined to peripheral blood and has the normal 46,XY male constitution. This was further confirmed by his normal male stature and normal testicular endocrine function. The 45X cell line is likely due to his receiving these cells passively from his twin sister via placental anastomoses in utero. The exposure to these 45,X cells during development may have had an impact on his spermatogenesis. © 2007 Wiley‐Liss, Inc.
Trisomy 9 mosaicism and XX sex reversal
Authors: Solomon Benjamin D. , Turner Clesson E. , Klugman Darren , Sparks Susan E. 
Published: October 2007

AbstractWe report on a case of a phenotypic male infant who was referred to the genetics service due to dysmorphic features and congenital cardiac anomalies. Dysmorphic features included low‐set, posteriorly rotated ears with squared, simple helices, midface hypoplasia, a broad nasal root with wide nasal alae, small mouth, micrognathia, short neck, overlapping fingers, rocker‐bottom feet, prominent heels, and hypoplastic toenails. The phallus was normal and no testes were palpable in the scrotal sac. Cardiac anomalies included tricuspid atresia and a ventricular septal defect. The karyotype was 46,XX. Diagnostic microarray demonstrated evidence for trisomy 9 mosaicism, metaphase FISH revealed trisomy 9 on 7% of cultured cells, and interphase FISH analysis of a peripheral blood smear showed trisomy 9 in 78% of the cells examined. This is the first reported case of XX sex reversal in a patient with trisomy 9 mosaicism. © 2007 Wiley‐Liss, Inc.
The timing of eruption of the primary dentition in Nigerian children
Authors: Folayan Morenike , Owotade Foluso , Adejuyigbe Ebun , Sen Saunak , Lawal Biola , Ndukwe Kizito 
Published: October 2007

AbstractThe objectives of this study were to clarify the eruption time and sequence for primary teeth in Nigerian children. It also investigated the effect of sex and socioeconomic status on the timing and sequence of eruption. A random sample of 1,657 children from ages of 3–40 months were examined—921 (55.6%) males and 736 (44.4%) females. The age of eruption of the teeth was estimated using probit regression. The results show that there was no effect of sex, socioeconomic status or breastfeeding status on the timing of eruption and pattern of teeth eruption in Nigerian children. Left and right teeth had similar eruption times. Eruption times of the lateral incisor, canine, and molars were similar for upper and lower teeth. Interpopulation studies however showed that though the sequence of eruption of primary teeth in Nigerian population is similar to that of their peers in other compared populations, there are observable sex differences in the timing of tooth eruption. Am J Phys Anthropol, 2007. © 2007 Wiley‐Liss, Inc.
Dental caries prevalence as evidence for agriculture and subsistence variation during the Yayoi period in prehistoric Japan: Biocultural interpretations of an economy in transition
Authors: Temple Daniel H. , Larsen Clark Spencer 
Published: October 2007

AbstractThe Yayoi period represents the earliest point of agricultural dependence in Japan, dating from ∼2500 BP to AD 300. Yayoi period people consumed wet‐rice as a primary subsistence base. This article uses dental caries prevalence to interpret the biocultural implications of agriculture among these people by testing the following hypotheses: 1) Yayoi period agriculturalists had greater frequencies of carious teeth than Jomon period foragers, 2) regional variation in carious tooth frequencies will be observed among Yayoi period agriculturalists, while 3) variation in carious tooth frequencies will be observed between male and female agriculturalists. Statistically significant differences in carious teeth were observed between the agriculturalists from Southern Honshu and all other samples. These differences suggest greater reliance on cariogenic plants among farmers from Southern Honshu and are consistent with an agricultural economy. The people of the Yayoi period from Tanegashima Island and Northern Kyushu did not have significantly different carious tooth frequencies compared to Jomon period foragers. This suggests that rice alone was not a more cariogenic dietary substance than those consumed by Jomon period foragers but a cariogenic food nonetheless. Dietary heterogeneity between the prehistoric people of the Yayoi period from Southern Honshu and those from Northern Kyushu and Tanegashima Island is also inferred from these differences. Significantly greater frequencies of carious teeth among older aged Yayoi period females compared with males suggest dietary differences between the sexes. Am J Phys Anthropol, 2007. © 2007 Wiley‐Liss, Inc.
New Jersey ethics opinion will alter how neutrals operate at mediation centers, highlighting the nature and structure not only of their relationships, but also the parties' obligations
Authors: Isaacs Hanan M. , Katz Jennifer L. B. 
Published: October 2007

AbstractA New Jersey State Supreme Court committee opinion puts new obligations on consumers of ADR provider services, and neutrals. Hanan M. Isaacs, of Princeton, N.J., and Jennifer L. B. Katz, of Buffalo, N.Y., discuss what to look for in your local rules
Organische Umsetzungen an metallorganischen σ‐Aryl‐Komplexen
Authors: Gagliardo Marcella , Snelders Dennis J. M. , Chase Preston A. , Klein Gebbink Robertus J. M. , van Klink Gerard P. M. , van Koten Gerard 
Published: October 2007

AbstractDieser Aufsatz gibt einen Überblick über neue Entwicklungen auf dem Gebiet der metallvermittelten organischen Umsetzungen an metallorganischen σ‐Aryl‐Komplexen. Die allgemeine Vorstellung, dass M‐C‐σ‐Bindungen kinetisch labil, hoch reaktiv und unvereinbar mit typischen Reaktionsbedingungen der organischen Synthese sind, hat die Anwendung dieser Synthesestrategien bisher eingeschränkt. Allerdings werden metallvermittelte organische Umsetzungen an metallgebundenen σ‐Aryl‐Fragmenten immer häufiger in industriellen und Laborsynthesen eingesetzt. Die Einfachheit und im Allgemeinen guten Ausbeuten dieser Methoden sind sehr attraktiv für den Aufbau funktionalisierter metallorganischer Bausteine, die als photochemische molekulare Funktionseinheiten, Biosensoren und ‐konjugate sowie molekulare Schalter genutzt werden könnten. Dieser Aufsatz richtet sich daher an eine breitere Leserschaft und soll zum Gebrauch dieser Strategien ermutigen.