bluuebunny/crossref_metadata_2025_split huggingface dataset:
Piloting co-developed behaviour change interventions to reduce exposure to air pollution and improve self-reported asthma-related health
Authors: McCarron Amy , Semple Sean , Swanson Vivien , Gillespie Colin , Braban Christine , Price Heather D. 
Published: April 2024

Abstract
Background
Exposure to air pollution can exacerbate asthma with immediate and long-term health consequences. Behaviour changes can reduce exposure to air pollution, yet its ‘invisible’ nature often leaves individuals unaware of their exposure, complicating the identification of appropriate behaviour modifications. Moreover, making health behaviour changes can be challenging, necessitating additional support from healthcare professionals.

Objective
This pilot study used personal exposure monitoring, data feedback, and co-developed behaviour change interventions with individuals with asthma, with the goal of reducing personal exposure to PM2.5 and subsequently improving asthma-related health.

Methods
Twenty-eight participants conducted baseline exposure monitoring for one-week, simultaneously keeping asthma symptom and medication diaries (previously published in McCarron et al., 2023). Participants were then randomised into control (n = 8) or intervention (n = 9) groups. Intervention participants received PM2.5 exposure feedback and worked with researchers to co-develop behaviour change interventions based on a health behaviour change programme which they implemented during the follow-up monitoring week. Control group participants received no feedback or intervention during the study.

Results
All interventions focused on the home environment. Intervention group participants reduced their at-home exposure by an average of 5.7 µg/m³ over the monitoring week (−23.0 to +3.2 µg/m³), whereas the control group had a reduction of 4.7 µg/m³ (−15.6 to +0.4 µg/m³). Furthermore, intervention group participants experienced a 4.6% decrease in participant-hours with reported asthma symptoms, while the control group saw a 0.5% increase. Similarly, the intervention group’s asthma-related quality of life improved compared to the control group.

Impact statement
This pilot study investigated a novel behaviour change intervention, utilising personal exposure monitoring, data feedback, and co-developed interventions guided by a health behaviour change programme. The study aimed to reduce personal exposure to fine particulate matter (PM2.5) and improve self-reported asthma-related health. Conducting a randomised controlled trial with 28 participants, co-developed intervention successfully targeted exposure peaks within participants’ home microenvironments, resulting in a reduction in at-home personal exposure to PM2.5 and improving self-reported asthma-related health. The study contributes valuable insights into the environmental exposure-health relationship and highlights the potential of the intervention for individual-level decision-making to protect human health.
Dentate gyrus is needed for memory retrieval
Authors: Carretero-Guillén Alejandro , Treviño Mario , Gómez-Climent María Ángeles , Dogbevia Godwin K. , Bertocchi Ilaria , Sprengel Rolf , Larkum Matthew E. , Vlachos Andreas , Gruart Agnès , Delgado-García José M. , Hasan Mazahir T. 
Published: April 2024

AbstractThe hippocampus is crucial for acquiring and retrieving episodic and contextual memories. In previous studies, the inactivation of dentate gyrus (DG) neurons by chemogenetic- and optogenetic-mediated hyperpolarization led to opposing conclusions about DG’s role in memory retrieval. One study used Designer Receptors Exclusively Activated by Designer Drugs (DREADD)-mediated clozapine N-oxide (CNO)-induced hyperpolarization and reported that the previously formed memory was erased, thus concluding that denate gyrus is needed for memory maintenance. The other study used optogenetic with halorhodopsin induced hyperpolarization and reported and dentate gyrus is needed for memory retrieval. We hypothesized that this apparent discrepancy could be due to the length of hyperpolarization in previous studies; minutes by optogenetics and several hours by DREADD/CNO. Since hyperpolarization interferes with anterograde and retrograde neuronal signaling, it is possible that the memory engram in the dentate gyrus and the entorhinal to hippocampus trisynaptic circuit was erased by long-term, but not with short-term hyperpolarization. We developed and applied an advanced chemogenetic technology to selectively silence synaptic output by blocking neurotransmitter release without hyperpolarizing DG neurons to explore this apparent discrepancy. We performed in vivo electrophysiology during trace eyeblink in a rabbit model of associative learning. Our work shows that the DG output is required for memory retrieval. Based on previous and recent findings, we propose that the actively functional anterograde and retrograde neuronal signaling is necessary to preserve synaptic memory engrams along the entorhinal cortex to the hippocampal trisynaptic circuit.
Role of liver FGF21-KLB signaling in ketogenic diet-induced amelioration of hepatic steatosis
Authors: Guo Wanrong , Cao Huanyi , Shen Yunfeng , Li Wuguo , Wang Wei , Cheng Lidan , Cai Mengyin , Xu Fen 
Published: April 2024

Abstract
Background
The effectiveness of ketogenic diet (KD) in ameliorating fatty liver has been established, although its mechanism is under investigation. Fibroblast growth factor 21 (FGF21) positively regulates obesity-associated metabolic disorders and is elevated by KD. FGF21 conventionally initiates its intracellular signaling via receptor β-klotho (KLB). However, the mechanistic role of FGF21-KLB signaling for KD-ameliorated fatty liver remains unknown. This study aimed to delineate the critical role of FGF21 signaling in the ameliorative effects of KD on hepatic steatosis.

Methods
Eight-week-old C57BL/6 J mice were fed a chow diet (CD), a high-fat diet (HFD), or a KD for 16 weeks. Adeno-associated virus-mediated liver-specific KLB knockdown mice and control mice were fed a KD for 16 weeks. Phenotypic assessments were conducted during and after the intervention. We investigated the mechanism underlying KD-alleviated hepatic steatosis using multi-omics and validated the expression of key genes.

Results
KD improved hepatic steatosis by upregulating fatty acid oxidation and downregulating lipogenesis. Transcriptional analysis revealed that KD dramatically activated FGF21 pathway, including KLB and fibroblast growth factor receptor 1 (FGFR1). Impairing liver FGF21 signaling via KLB knockdown diminished the beneficial effects of KD on ameliorating fatty liver, insulin resistance, and regulating lipid metabolism.

Conclusion
KD demonstrates beneficial effects on diet-induced metabolic disorders, particularly on hepatic steatosis. Liver FGF21-KLB signaling plays a critical role in the KD-induced amelioration of hepatic steatosis.
Infection with SARS-CoV-2 can cause pancreatic impairment
Authors: Deng Wei , Bao Linlin , Song Zhiqi , Zhang Ling , Yu Pin , Xu Yanfeng , Wang Jue , Zhao Wenjie , Zhang Xiuqin , Han Yunlin , Li Yanhong , Liu Jiangning , Lv Qi , Liang Xujian , Li Fengdi , Qi Feifei , Deng Ran , Wang Siyuan , Xiong Yibai , Xiao Ruiping , Wang Hongyang , Qin Chuan 
Published: April 2024

AbstractEvidence suggests associations between COVID-19 patients or vaccines and glycometabolic dysfunction and an even higher risk of the occurrence of diabetes. Herein, we retrospectively analyzed pancreatic lesions in autopsy tissues from 67 SARS-CoV-2 infected non-human primates (NHPs) models and 121 vaccinated and infected NHPs from 2020 to 2023 and COVID-19 patients. Multi-label immunofluorescence revealed direct infection of both exocrine and endocrine pancreatic cells by the virus in NHPs and humans. Minor and limited phenotypic and histopathological changes were observed in adult models. Systemic proteomics and metabolomics results indicated metabolic disorders, mainly enriched in insulin resistance pathways, in infected adult NHPs, along with elevated fasting C-peptide and C-peptide/glucose ratio levels. Furthermore, in elder COVID-19 NHPs, SARS-CoV-2 infection causes loss of beta (β) cells and lower expressed-insulin in situ characterized by islet amyloidosis and necrosis, activation of α-SMA and aggravated fibrosis consisting of lower collagen in serum, an increase of pancreatic inflammation and stress markers, ICAM-1 and G3BP1, along with more severe glycometabolic dysfunction. In contrast, vaccination maintained glucose homeostasis by activating insulin receptor α and insulin receptor β. Overall, the cumulative risk of diabetes post-COVID-19 is closely tied to age, suggesting more attention should be paid to blood sugar management in elderly COVID-19 patients.
A life cycle analysis of the environmental impact of procurement, waste and water in the dental practice
Authors: Suresh Peter , Crotty John , Tesanovic Sonja , Alaweed Othman , Doyle Sadhbh , Kiandee Mikra , Hayes Emily , Umeh Vanessa , Khalilinejad Bita , Duane Brett 
Published: April 2024

AbstractBackground Health care is a significant contributor to climate change. Global pressure for a change towards a more sustainable way of providing dental health care has resulted in the creation of the Green Impact Toolkit, which is comprised of a list of suggested changes that dental practices can make to become more sustainable in a number of categories, such as procurement, waste and water.Aims To compare the effectiveness of changes suggested by the Green Impact Toolkit.Materials and methods A comparative life cycle assessment (LCA) was conducted using the Ecoinvent database v3.8 and these data were processed using OpenLCA v1.10.3 software.Results The carbon footprint per patient was significantly reduced after the recommendations were implemented. For instance, using water from a rainwater collection tank instead of the mains supply saved 30 g CO2eq (carbon dioxide equivalents) per patient, a 90% reduction in carbon footprint.Discussion This comparative LCA identified some effective changes which can be easily made by a dental practice. Nevertheless, some actions require some initial financial investment and may be difficult to implement in a busy modern dental practice setting.Conclusion The findings from this study can be used to guide dental practices to making choices which are more sustainable and eco-friendly in the future.
A bone adhesive enhances osteoporotic fracture repair by regulating bone homeostasis
Authors: Su Shenghui , Xie Jiajun , Gao Jian , Liu Shencai , Dong Xieping , Li Jianwei , Gao Zhong Feng , Chen Keyuan , Liu Weilu 
Published: April 2024

Abstract
Patients suffering from osteoporotic fractures often require effective fixation and subsequent bone repair. However, the currently available materials are functionally limited and often fail to improve outcomes in this patient population. In this study, we developed orthopedic adhesives doped with romosozumab-loaded mesoporous bioactive glass nanoparticles to aid in osteoporotic fracture fixation and restore dysregulated bone homeostasis. These adhesives were designed to promote osteoblast formation while simultaneously inhibiting osteoclastic bone-resorbing activity, thus working synergistically to promote the healing of osteoporotic fractures. Orthopedic adhesives exhibit injectability, reversible adhesiveness, and malleability, enhancing their adaptability to complex clinical scenarios. Furthermore, the release of romosozumab from mesoporous bioactive glass nanoparticles accelerated osteogenesis and inhibited osteoclastogenesis, delaying the bone resorption process. This dual action contributes to the regulation of bone regeneration and remodeling. Notably, our orthopedic adhesive could restore the disrupted bone homeostasis associated with osteoporotic fractures.
Origami metamaterials for ultra-wideband and large-depth reflection modulation
Authors: Song Zicheng , Zhu Juan-Feng , Wang Xianchao , Zhang Ruicong , Min Pingping , Cao Wenxin , He Yurong , Han Jiecai , Wang Tianyu , Zhu Jiaqi , Wu Lin , Qiu Cheng-Wei 
Published: April 2024

AbstractThe dynamic control of electromagnetic waves is a persistent pursuit in modern industrial development. The state-of-the-art dynamic devices suffer from limitations such as narrow bandwidth, limited modulation range, and expensive features. To address these issues, we fuse origami techniques with metamaterial design to achieve ultra-wideband and large-depth reflection modulation. Through a folding process, our proposed metamaterial achieves over 10-dB modulation depth over 4.96 – 38.8 GHz, with a fractional bandwidth of 155% and tolerance to incident angles and polarizations. Its ultra-wideband and large-depth reflection modulation performance is verified through experiments and analyzed through multipole decomposition theory. To enhance its practical applicability, transparent conductive films are introduced to the metamaterial, achieving high optical transparency (>87%) from visible to near-infrared light while maintaining cost-effectiveness. Benefiting from lightweight, foldability, and low-cost properties, our design shows promise for extensive satellite communication and optical window mobile communication management.
Integrated proteogenomic and metabolomic characterization of papillary thyroid cancer with different recurrence risks
Authors: Qu Ning , Chen Di , Ma Ben , Zhang Lijun , Wang Qiuping , Wang Yuting , Wang Hongping , Ni Zhaoxian , Wang Wen , Liao Tian , Xiang Jun , Wang Yulong , Jin Shi , Xue Dixin , Wu Weili , Wang Yu , Ji Qinghai , He Hui , Piao Hai-long , Shi Rongliang 
Published: April 2024

AbstractAlthough papillary thyroid cancer (PTC) has a good prognosis, its recurrence rate is high and remains a core concern in the clinic. Molecular factors contributing to different recurrence risks (RRs) remain poorly defined. Here, we perform an integrative proteogenomic and metabolomic characterization of 102 Chinese PTC patients with different RRs. Genomic profiling reveals that mutations in MUC16 and TERT promoter as well as multiple gene fusions like NCOA4-RET are enriched by the high RR. Integrative multi-omics analyses further describe the multi-dimensional characteristics of PTC, especially in metabolism pathways, and delineate dominated molecular patterns of different RRs. Moreover, the PTC patients are clustered into four subtypes (CS1: low RR and BRAF-like; CS2: high RR and metabolism type, worst prognosis; CS3: high RR and immune type, better prognosis; CS4: high RR and BRAF-like) based on the omics data. Notably, the subtypes display significant differences considering BRAF and TERT promoter mutations, metabolism and immune pathway profiles, epithelial cell compositions, and various clinical factors (especially RRs and prognosis) as well as druggable targets. This study can provide insights into the complex molecular characteristics of PTC recurrences and help promote early diagnosis and precision treatment of recurrent PTC.
Evidence for a competitive relationship between executive functions and statistical learning
Authors: Pedraza Felipe , Farkas Bence C. , Vékony Teodóra , Haesebaert Frederic , Phelipon Romane , Mihalecz Imola , Janacsek Karolina , Anders Royce , Tillmann Barbara , Plancher Gaën , Németh Dezső 
Published: April 2024

AbstractThe ability of the brain to extract patterns from the environment and predict future events, known as statistical learning, has been proposed to interact in a competitive manner with prefrontal lobe-related networks and their characteristic cognitive or executive functions. However, it remains unclear whether these cognitive functions also possess a competitive relationship with implicit statistical learning across individuals and at the level of latent executive function components. In order to address this currently unknown aspect, we investigated, in two independent experiments (NStudy1 = 186, NStudy2 = 157), the relationship between implicit statistical learning, measured by the Alternating Serial Reaction Time task, and executive functions, measured by multiple neuropsychological tests. In both studies, a modest, but consistent negative correlation between implicit statistical learning and most executive function measures was observed. Factor analysis further revealed that a factor representing verbal fluency and complex working memory seemed to drive these negative correlations. Thus, the antagonistic relationship between implicit statistical learning and executive functions might specifically be mediated by the updating component of executive functions or/and long-term memory access.
BepiColombo observations of cold oxygen and carbon ions in the flank of the induced magnetosphere of Venus
Authors: Hadid L. Z. , Delcourt D. , Saito Y. , Fränz M. , Yokota S. , Fiethe B. , Verdeil C. , Katra B. , Leblanc F. , Fischer H. , Persson M. , Aizawa S. , André N. , Harada Y. , Fedorov A. , Fontaine D. , Krupp N. , Michalik H. , Berthelier J-J. , Krüger H. , Murakami G. , Matsuda S. , Heyner D. , Auster H.-U. , Richter I. , Mieth J. Z. D. , Schmid D. , Fischer D. 
Published: April 2024

AbstractOn 10 August 2021, the Mercury-bound BepiColombo spacecraft performed its second fly-by of Venus and provided a short-lived observation of its induced magnetosphere. Here we report results recorded by the Mass Spectrum Analyzer on board Mio, which reveal the presence of cold O+ and C+ with an average total flux of ~4 ± 1 × 104 cm−2 s−1 at a distance of about six planetary radii in a region that has never been explored before. The ratio of escaping C+ to O+ is at most 0.31 ± 0.2, implying that, in addition to atomic O+ ions, CO group ions or water group ions may be a source of the observed O+. Simultaneous magnetometer observations suggest that these planetary ions were in the magnetosheath flank in the vicinity of the magnetic pileup boundary downstream. These results have important implications regarding the evolution of Venus’s atmosphere and, in particular, the evolution of water on the surface of the planet.
Rapid Laurentide Ice Sheet growth preceding the Last Glacial Maximum due to summer snowfall
Authors: Niu Lu , Knorr Gregor , Krebs-Kanzow Uta , Gierz Paul , Lohmann Gerrit 
Published: April 2024

AbstractThere has been extensive research into the nonlinear responses of the Earth system to astronomical forcing during the last glacial cycle. However, the speed and spatial geometry of ice sheet expansion to its largest extent at the Last Glacial Maximum 21 thousand years ago remains uncertain. Here we use an Earth system model with interactive ice sheets to show that distinct initial North American (Laurentide) ice sheets at 38 thousand years ago converge towards a configuration consistent with the Last Glacial Maximum due to feedbacks between atmospheric circulation and ice sheet geometry. Notably, ice advance speed and spatial pattern in our model are controlled by the amount of summer snowfall, which is dependent on moisture transport pathways from the North Atlantic warm pool linked to ice sheet geometry. The consequence of increased summer snowfall on the surface mass balance of the ice sheet is not only the direct increase in accumulation but the indirect reduction in melt through the snow/ice–albedo feedback. These feedbacks provide an effective mechanism for ice growth for a range of initial ice sheet states and may explain the rapid North American ice volume increase during the last ice age and potentially driving growth during previous glacial periods.
Structure of the native γ-tubulin ring complex capping spindle microtubules
Authors: Dendooven Tom , Yatskevich Stanislau , Burt Alister , Chen Zhuo A. , Bellini Dom , Rappsilber Juri , Kilmartin John V. , Barford David 
Published: April 2024

AbstractMicrotubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTuRC presents a ring of γ-tubulin subunits to seed nucleation of exclusively 13-protofilament MTs, adopting an active closed conformation to function as a perfect geometric template for MT nucleation. Our cryo-electron tomography reconstruction revealed that a coiled-coil protein staples the first row of α/β-tubulin of the MT to alternating positions along the γ-tubulin ring of γTuRC. This positioning of α/β-tubulin onto γTuRC suggests a role for the coiled-coil protein in augmenting γTuRC-mediated MT nucleation. Based on our results, we describe a molecular model for budding yeast γTuRC activation and MT nucleation.
A dataset for soil organic carbon in agricultural systems for the Southeast Asia region
Authors: Gomez Federico , Carcedo Ana , Mean Chan Makara , Reyes Manuel , Hok Lyda , Tivet Florent , Seng Vang , Vara Prasad P. V. , Ciampitti Ignacio 
Published: April 2024

AbstractThe determination of changes in soil organic carbon (SOC) content under different cropping systems is necessary for policy development oriented towards soil conservation, C sequestration, and future C credit markets. The aim of this study was to generate an open SOC dataset resulting from a systematic literature search related to the agricultural systems for Southeast Asia. The dataset has 209 articles and 4341 observations on soils of cropping systems in this region from articles published between 1987 and 2023. This dataset included different management practices, land uses, soil sampling depth, and length of SOC content assessment. In addition, inherent features of crop production reported in the experiments were included in the dataset. This dataset can be applied to quantify and compare the impact of different land uses or management practices on SOC content, providing foundational knowledge towards identifying sustainable practices. Lastly, it is a useful guide for future regional SOC sequestration policies and the development of C credit markets.
The transcriptome assembly of the European freshwater mussel Unio elongatulus C. Pfeiffer, 1825
Authors: Gomes-dos-Santos André , Fonseca Elza , Riccardi Nicoletta , Hinzmann Mariana , Lopes-Lima Manuel , Froufe Elsa 
Published: April 2024

AbstractFreshwater mussels of the order Unionida are a global conservation concern. Species of this group are strictly freshwater, sessile, slow-growing animals and, extremely sensitive to environmental changes. Human-mediated changes in freshwater habitats are imposing enormous pressure on the survival of freshwater mussels. Although a few flagship species are protected in Europe, other highly imperilled species receive much less attention. Moreover, knowledge about biology, ecology, and evolution and proper conservation assessments of many European species are still sparse. This knowledge gap is further aggravated by the lack of genomic resources available, which are key tools for conservation. Here we present the transcriptome assembly of Unio elongatulus C. Pfeiffer, 1825, one of the least studied European freshwater mussels. Using the individual sequencing outputs from eight physiologically representative mussel tissues, we provide an annotated panel of tissue-specific Relative Gene Expression profiles. These resources are pivotal to studying the species’ biological and ecological features, as well as helping to understand its vulnerability to current and future threats.