bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating Uncertainties in the Projection of Future Drought
Authors: Burke Eleanor J. , Brown Simon J. 
Published: April 2008

Abstract
The uncertainty in the projection of future drought occurrence was explored for four different drought indices using two model ensembles. The first ensemble expresses uncertainty in the parameter space of the third Hadley Centre climate model, and the second is a multimodel ensemble that additionally expresses structural uncertainty in the climate modeling process. The standardized precipitation index (SPI), the precipitation and potential evaporation anomaly (PPEA), the Palmer drought severity index (PDSI), and the soil moisture anomaly (SMA) were derived for both a single CO2 (1×CO2) and a double CO2 (2×CO2) climate. The change in moderate drought, defined by the 20th percentile of the relevant 1×CO2 distribution, was calculated. SPI, based solely on precipitation, shows little change in the proportion of the land surface in drought. All the other indices, which include a measure of the atmospheric demand for moisture, show a significant increase with an additional 5%–45% of the land surface in drought. There are large uncertainties in regional changes in drought. Regions where the precipitation decreases show a reproducible increase in drought across ensemble members and indices. In other regions the sign and magnitude of the change in drought is dependent on index definition and ensemble member, suggesting that the selection of appropriate drought indices is important for impact studies.
Bursting-Layer Modeling Based on the Assumption of the Averaged Sea Surface for Strong Wind-Driven Currents
Authors: Murakami Tomokazu , Yasuda Takashi 
Published: April 2008

Abstract
In the sea, which is affected by strong winds that cover the water surface with wind-wave breakers, the sea surface layer, called the bursting layer by authors, is generated immediately below the mean water level. For treatment of strong wind-driven currents, it is necessary to model the bursting layer correctly based on observed data. However, an essential difficulty occurs in that, because of large water surface displacement caused by developed wind waves, water particle velocities above the wave trough level cannot be measured continuously in the Eulerian coordinates when measuring the velocity of strong wind-driven currents. Consequently, it is impossible to apply the Reynolds average rule to the velocity field above the wave trough level.
In this study, an experiment that uses a wind-wave tank with a double bottom is performed to measure the horizontal velocity of currents driven only by the wind stress. The vertical distribution up to the mean water level is determined to make its vertically integrated value agree with the total mass flux of the wind-driven currents. Furthermore, the vertical distribution of the Reynolds stress is derived by solving a Reynolds equation; its solution agrees with the given velocity distribution. Numerical simulation was performed to reproduce the strong wind-driven currents. Results showed that the bursting-layer model can correctly calculate the steep vertical distribution of the current velocity in the bursting layer.
Wave-Coherent Air–Sea Heat Flux
Authors: Veron Fabrice , Melville W. Kendall , Lenain Luc 
Published: April 2008

Abstract
Air–sea fluxes of heat and momentum play a crucial role in weather, climate, and the coupled general circulation of the oceans and atmosphere. Much progress has been made to quantify momentum transfer from the atmosphere to the ocean for a wide range of wind and wave conditions. Yet, despite the fact that global heat budgets are now at the forefront of current research in atmospheric, oceanographic, and climate problems and despite the good research progress in recent years, much remains to be done to better understand and quantify air–sea heat transfer. It is well known that ocean-surface waves may support momentum transfer from the atmosphere to the ocean, but the role of the waves in heat transfer has been ambiguous and poorly understood. Here, evidence is presented that there are surface wave–coherent components of both the sensible and the latent heat fluxes. Presented here are data from three field experiments that show modulations of temperature and humidity at the surface and at 10–14 m above the surface, which are coherent with the surface wave field. The authors show that the phase relationship between temperature and surface displacement is a function of wind speed. At a 10–12-m elevation, a wave-coherent heat transfer of O(1) W m−2 is found, dominated by the latent heat transfer, as well as wave-coherent fractional contributions to the total heat flux (the sum of latent and sensible heat fluxes) of up to 7%. For the wind speeds and wave conditions of these experiments, which encompass the range of global averages, this wave contribution to total heat flux is comparable in magnitude to the atmospheric heat fluxes commonly attributed to the effects of greenhouse gases or aerosols. By analogy with momentum transfer, the authors expect the wave-coherent heat transfer to decay with height over scales on the order of k−1, where k is the characteristic surface wavenumber; therefore, it is also expected that measurements at elevations of O(10) m may underestimate the contribution of the wave-induced heat flux to the atmosphere.
Orographic Clouds in Terrain-Blocked Flows: An Idealized Modeling Study
Authors: Galewsky Joseph 
Published: April 2008

Abstract
Idealized numerical simulations of moist strongly stratified flow over topography are used to study the processes that control orographic clouds in terrain-blocked flows as a joint function of the nondimensional flow parameter Nh/U, the horizontal topographic aspect ratio β, and the Rossby radius of deformation Nh/f. The simulations show the competition between enhanced upstream condensation in the secondary vertically propagating gravity wave and the reduction of condensation owing to enhanced low-level flow deflection. As Nh/U increases above about 1.5, the tendency for flow to be deflected around the barrier reduces cloud formation in the primary gravity wave over the ridge, while increasing β expands low-level clouds over a broader upstream area. Ice clouds may form aloft in the secondary vertically propagating gravity wave and extend upstream for several hundred kilometers. In terrain-blocked flows, more than half of the condensate mass develops upstream of the barrier in the secondary gravity wave, while in unblocked flows most of the condensate is downstream of the barrier in the primary lee wave. In 2D, none of the flow can be diverted around the barrier, and it therefore produces a much more vigorous hydrologic cycle than over long (β = 8) 3D ridges, increasing upstream lifting and cloud water content by at least a factor of 2, and generating primary wave clouds that are not produced in the 3D case. Rotation reduces the upstream extent of condensation in blocked flows to a region on the order of the radius of deformation and in 3D induces a marked asymmetry in the lifting and condensation upstream of the terrain.
NASA Cold Land Processes Experiment (CLPX 2002/03): Local Scale Observation Site
Authors: Hardy Janet , Davis Robert , Koh Yeohoon , Cline Don , Elder Kelly , Armstrong Richard , Marshall Hans-Peter , Painter Thomas , Saint-Martin Gilles Castres , DeRoo Roger , Sarabandi Kamal , Graf Tobias , Koike Toshio , McDonald Kyle 
Published: April 2008

Abstract
The local scale observation site (LSOS) is the smallest study site (0.8 ha) of the 2002/03 Cold Land Processes Experiment (CLPX) and is located within the Fraser mesocell study area. It was the most intensively measured site of the CLPX, and measurements here had the greatest temporal component of all CLPX sites. Measurements made at the LSOS were designed to produce a comprehensive assessment of the snow, soil, and vegetation characteristics viewed by the ground-based remote sensing instruments. The objective of the ground-based microwave remote sensing was to collect time series of active and passive microwave spectral signatures over snow, soil, and forest, which is coincident with the intensive physical characterization of these features. Ground-based remote sensing instruments included frequency modulated continuous wave (FMCW) radars operating over multiple microwave bandwidths; the Ground-Based Microwave Radiometer (GBMR-7) operating at channels 18.7, 23.8, 36.5, and 89 GHz; and in 2003, an L-, C-, X- and Ku-band scatterometer radar system. Snow and soil measurements included standard snow physical properties, snow wetness, snow depth transects, and soil moisture. The stem and canopy temperature and xylem sap flux of several trees were monitored continuously. Five micrometeorological towers monitored ambient conditions and provided forcing datasets for 1D snow and soil models. Arrays of pyranometers (0.3–3 μm) and a scanning thermal radiometer (8–12 μm) characterized the variability of radiative receipt in the forests. A field spectroradiometer measured the hyperspectral hemispherical-directional reflectance of the snow surface. These measurements, together with the ground-based remote sensing, provide the framework for evaluating and improving microwave radiative transfer models and coupling them to land surface models. The dataset is archived at the National Snow and Ice Data Center (NSIDC) in Boulder, Colorado.
The Effect of Breaking Waves on a Coupled Model of Wind and Ocean Surface Waves. Part I: Mature Seas
Authors: Kukulka Tobias , Hara Tetsu 
Published: April 2008

Abstract
This is the first of a two-part investigation of a coupled wind and wave model that includes the enhanced form drag of breaking waves. In Part I here the model is developed and applied to mature seas. Part II explores the solutions in a wide range of wind and wave conditions, including growing seas. Breaking and nonbreaking waves induce air-side fluxes of momentum and energy above the air–sea interface. By balancing air-side momentum and energy and by conserving wave energy, coupled nonlinear advance–delay differential equations are derived, which govern simultaneously the wave and wind field. The system of equations is closed by introducing a relation between the wave height spectrum and wave dissipation due to breaking. The wave dissipation is proportional to nonlinear wave interactions, if the wave curvature spectrum is below the “threshold saturation level.” Above this threshold the wave dissipation rapidly increases so that the wave height spectrum is limited. The coupled model is applied to mature wind-driven seas for which the wind forcing only occurs in the equilibrium range away from the spectral peak. Modeled wave height curvature spectra as functions of wavenumber k are consistent with observations and transition from k1/2 at low wavenumbers to k0 at high wavenumbers. Breaking waves affect only weakly the wave height spectrum. Furthermore, the wind input to waves is dominated by nonbreaking waves closer to the spectral peak. Shorter breaking waves, however, can support a significant fraction, which increases with wind speed, of the total air–sea momentum flux.
An Intercomparison of Ground-Based Solar FTIR Measurements of Atmospheric Gases at Eureka, Canada
Authors: Paton-Walsh C. , Mittermeier R. L. , Bell W. , Fast H. , Jones N. B. , Meier A. 
Published: April 2008

Abstract
The authors report the results of an intercomparison of vertical column amounts of hydrogen chloride (HCl), hydrogen fluoride (HF), nitrous oxide (N2O), nitric acid (HNO3), methane (CH4), ozone (O3), carbon dioxide (CO2), and nitrogen (N2) derived from the spectra recorded by two ground-based Fourier transform infrared (FTIR) spectrometers operated side-by-side using the sun as a source. The procedure used to record spectra and derive vertical column amounts follows the format of previous instrument intercomparisons organized by the Network for the Detection of Atmospheric Composition Change (NDACC), formerly known as the Network for Detection of Stratospheric Change (NDSC).
For most gases the differences were typically around 3%, and in about half of the results the error bars given by the standard deviation of the measurements from each instrument did not overlap. The worst level of agreement was for HF where differences of over 5% were typical. The level of agreement achieved during this intercomparison is a little worse than that achieved in previous intercomparisons between ground-based FTIR spectrometers.
Characteristics and outcomes of patients with chronic myeloid leukemia and T315I mutation following failure of imatinib mesylate therapy
Authors: Jabbour Elias , Kantarjian Hagop , Jones Dan , Breeden Megan , Garcia-Manero Guillermo , O'Brien Susan , Ravandi Farhad , Borthakur Gautam , Cortes Jorge 
Published: April 2008

AbstractChronic myeloid leukemia (CML) with T315I mutation has been reported to have poor prognosis. We analyzed 27 patients with T315I, including 20 who developed T315I after imatinib failure (representing 11% of 186 patients with imatinib failure), and 7 of 23 who developed new mutations after second tyrosine kinase inhibitor (TKI). Median follow-up from mutation detection was 18 months. At the time of T315I detection, 10 were in chronic phase (CP), 9 in accelerated phase, and 8 in blast phase. Except for the lack of response to second TKIs (P = .002), there was no difference in patient characteristics and outcome between patients with T315I and those with other or no mutations. Patients in CP had a 2-year survival rate of 87%. Although the T315I mutation is resistant to currently available TKIs, survival of patients with T315I remains mostly dependent on the stage of the disease, with many CP patients having an indolent course.
Structure and expression pattern of Oct4 gene are conserved in vole Microtus rossiaemeridionalis
Authors: Medvedev Sergey P , Shevchenko Alexander I , Elisaphenko Eugene A , Nesterova Tatyana B , Brockdorff Neil , Zakian Suren M 
Published: April 2008

Abstract

Background
Oct4 is a POU-domain transcriptional factor which is essential for maintaining pluripotency in several mammalian species. The mouse, human, and bovine Oct4 orthologs display a high conservation of nucleotide sequence and genomic organization.

Results
Here we report an isolation of a common vole (Microtus rossiaemeridionalis) Oct4 ortholog. Organization and exon-intron structure of vole Oct4 gene are similar to the gene organization in other mammalian species. It consists of five exons and a regulatory region including the minimal promoter, proximal and distal enhancers. Promoter and regulatory regions of the vole Oct4 gene also display a high similarity to the corresponding regions of Oct4 in other mammalian species, and are active during the transient transfection within luciferase reporter constructs into mouse P19 embryonic carcinoma cells and TG-2a embryonic stem cells. The vole Oct4 gene expression is detectable starting from the morula stage and until day 17 of embryonic development.

Conclusion
Genomic organization of this gene and its intron-exon structure in vole are identical to those in all previously studied species: it comprises five exons and the regulatory region containing several conserved elements. The activity of the Oct4 gene in vole, as well as in mouse, is confined only to pluripotent cells.
Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity
Authors: Tenbusch Matthias , Kuate Seraphin , Tippler Bettina , Gerlach Nicole , Schimmer Simone , Dittmer Ulf , Überla Klaus 
Published: April 2008

AbstractBackgroundGranulocyte-macrophage colony-stimulating factor (GM-CSF) has shown promising results as a cytokine adjuvant for antiviral vaccines and in various models of tumor gene therapy. To explore whether the targeting of antigens to GM-CSF receptors on antigen-presenting cells enhances antigen-specific CD8 T-cell responses, fusion proteins of GM-CSF and ovalbumin (OVA) were expressed by DNA and adenoviral vector vaccines. In addition, bicistronic vectors allowing independent expression of the antigen and the cytokine were tested in parallel.ResultsIn vitro, the GM-CSF ovalbumin fusion protein (GM-OVA) led to the better stimulation of OVA-specific CD8+ T cells by antigen-presenting cells than OVA and GM-CSF given as two separate proteins. However, prime-boost immunizations of mice with DNA and adenoviral vector vaccines encoding GM-OVA suppressed CD8+ T-cell responses to OVA. OVA-specific IgG2a antibody levels were also reduced, while the IgG1 antibody response was enhanced. Suppression of CD8+ T cell responses by GM-OVA vaccines was associated with the induction of neutralizing antibodies to GM-CSF. In contrast, the coexpression of GM-CSF and antigens in DNA prime adenoviral boost immunizations led to a striking expansion of polyfunctional OVA-specific CD8+ T cells without the induction of autoantibodies.ConclusionThe induction of autoantibodies suggests a general note of caution regarding the use of highly immunogenic viral vector vaccines encoding fusion proteins between antigens and host proteins. In contrast, the expansion of polyfunctional OVA-specific CD8+ T cells after immunizations with bicistronic vectors further support a potential application of GM-CSF as an adjuvant for heterologous prime-boost regimens with genetic vaccines. Since DNA prime adenoviral vector boost regimenes are presently considered as one of the most efficient ways to induce CD8+ T cell responses in mice, non-human primates and humans, further enhancement of this response by GM-CSF is a striking observation.
Surfactant proteins SP-B and SP-C and their precursors in bronchoalveolar lavages from children with acute and chronic inflammatory airway disease
Authors: Tafel Oliver , Latzin Philipp , Paul Karl , Winter Tobias , Woischnik Markus , Griese Matthias 
Published: April 2008

Abstract

Background
The surfactant proteins B (SP-B) and C (SP-C) are important for the stability and function of the alveolar surfactant film. Their involvement and down-regulation in inflammatory processes has recently been proposed, but their level during neutrophilic human airway diseases are not yet known.

Methods
We used 1D-electrophoresis and Western blotting to determine the concentrations and molecular forms of SP-B and SP-C in bronchoalveolar lavage (BAL) fluid of children with different inflammatory airway diseases. 21 children with cystic fibrosis, 15 with chronic bronchitis and 14 with pneumonia were included and compared to 14 healthy control children.

Results
SP-B was detected in BAL of all 64 patients, whereas SP-C was found in BAL of all but 3 children; those three BAL fluids had more than 80% neutrophils, and in two patients, who were re-lavaged later, SP-C was then present and the neutrophil count was lower. SP-B was mainly present as a dimer, SP-C as a monomer. For both qualitative and quantitative measures of SP-C and SP-B, no significant differences were observed between the four evaluated patient groups.

Conclusion
Concentration or molecular form of SP-B and SP-C is not altered in BAL of children with different acute and chronic inflammatory lung diseases. We conclude that there is no down-regulation of SP-B and SP-C at the protein level in inflammatory processes of neutrophilic airway disease.
Morphology and function of cryopreserved whole ovine ovaries after heterotopic autotransplantation
Authors: Grazul-Bilska Anna T , Banerjee Jashoman , Yazici Ilker , Borowczyk Ewa , Bilski Jerzy J , Sharma Rakesh K , Siemionow Maria , Falcone Tommaso 
Published: April 2008

Abstract

Background
The objective of this study was to perform complex characterization of cryopreserved and then autotransplanted ovaries including determination of the ability to respond to in vivo follicle stimulating hormone (FSH)-treatment, fertilizability of retrieved oocytes, and morphology, vascularization, cellular proliferation and apoptosis in sheep.

Methods
Mature crossbred ewes were divided into two groups; an intact (control) group (n = 4), and autotransplanted group (n = 4) in which oophorectomy was performed laparoscopically and ovaries with intact vascular pedicles frozen, thawed and transplanted back into the same animal at a different site. Approximately five months after autotransplantation, estrus was synchronized, ewes were treated with FSH, and ovaries were collected. For all ovaries, number of visible follicles was determined, and collected cumulus oocyte complexes (COC) were matured and fertilized in vitro. Remaining ovarian tissues were fixed for evaluation of morphology, expression of factor VIII (marker of endothelial cells), vascular endothelial growth factor (VEGF; expressed by pericytes and smooth muscle cells), and smooth muscle cell actin (SMCA; marker of pericytes and smooth muscle cells), and cellular proliferation and apoptosis. Two fully functional ovaries were collected from each control ewe (total 8 ovaries).

Results
Out of eight autotransplanted ovaries, a total of two ovaries with developing follicles were found. Control ewes had 10.6 +/- 2.7 follicles/ovary, oocytes were in vitro fertilized and developed to the blastocyst stage. One autotransplanted ewe had 4 visible follicles from which 3 COC were collected, but none of them was fertilized. The morphology of autotransplanted and control ovaries was similar. In control and autotransplanted ovaries, primordial, primary, secondary, antral and preovulatory follicles were found along with fully functional vascularization which was manifested by expression of factor VIII, VEGF and SMCA. Proliferating cells were detected in follicles, and the rate of apoptosis was minimal in ovaries of control and autotransplanted ovaries.

Conclusion
These data demonstrate successful autotransplantation of a portion of frozen/thawed ovaries manifested by restoration of selected ovarian function including in vitro maturation of collected oocytes, presence of follicles from several stages of folliculogenesis and blood vessels expressing specific markers of vascularization, and proliferation and apoptosis of ovarian cells. Thus, heterotopic autotransplantation of a whole frozen/thawed ovary allows for development of preovulatory follicles, oocyte growth, and for restoration of vascularization and cellular function. However, additional improvements are required to enhance the efficiency of autotransplantation of frozen/thawed ovaries to produce more oocytes.