bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sexual Activity Influences the Secretion of Reproductive Hormones in the Stallion
Authors: Aurich C. , Gerlach T. , Hoppen H‐O. , Aurich J.E. 
Published: October 2007

ContentsThe aim of this study was to investigate the effect of sexual activity on concentrations of reproductive hormones in plasma of stallions. In the first experiment, two groups of stallions were monitored for secretion of luteinizing hormone (LH), testosterone and oestradiol from the beginning until shortly after the end of the breeding season. One group of animals were reserve stallions not used for breeding (group 1, n = 10), the other group consisted of active breeding sires (group 2, n = 8). Blood samples were withdrawn from March to August at 14‐day intervals. In sexually nonactive stallions (group 1), seasonal variations in LH, testosterone and oestradiol occurred and concentrations of these hormones reached a maximum in May (p < 0.05). In the breeding stallions (group 2), no significant changes in the concentrations of these hormones were found between March and August. Concentrations of LH and testosterone were significantly lower in breeding stallions than in reserve stallions at most blood sampling times (p < 0.05). In the reserve stallions, oestradiol concentrations were significantly higher than in the breeding stallions in April and in June (p < 0.05). In a second experiment, the effect of regular sexual activity (semen collection three times per week) on the concentration of LH, testosterone and oestradiol was tested in a group of breeding stallions after a period of sexual rest for several weeks. Blood samples were taken once daily starting the day before the first semen collection was performed. Testosterone concentration significantly decreased in the first days after semen collection started (p < 0.05), while LH secretion was only transiently decreased and no effects on oestradiol concentration were found. In both experiments, semen parameters were within the normal range of fertile stallions. No correlations between the sexual drive of the stallions and concentration of reproductive hormones occurred. It can be concluded that in the stallion the secretion of reproductive hormones is influenced by sexual activity. Regular semen collection seems to inhibit testosterone release by unknown mechanisms while the effects on LH and oestradiol secretion are less pronounced.
Transcriptional Activity and Ultrastructure in Bovine Oocytes
Authors: Hyttel P , Fair T , Avery B , Callesen H , Greve T 
Published: October 2007

ContentsThe oocyte growth phase includes development of organelles and inclusions as well as a period of oocyte transcription. Oocyte transcription including nucleolar function (rRNA‐synthesis) is activated in the secondary follicle and is maintained up to an oocyte diameter of about 110 μm in the 3 mm tertiary follicle. At a diameter of 100 to 110 μm, the oocyte gradually achieves the competence to undergo meiotic maturation and sustain embryonic development. In the dominant follicle the oocyte undergoes further ultrastructural modifications through a process that we refer to as «Capacitation». The final oocyte maturation up to metaphase II after LH stimulation of the ovulatory follicle is the culmination of the previous processes and equips the oocyte with the cell biological apparatus specialized for fertilization and initial embryonic development.
Follicular Growth is Characterized by Biochemical Interplay of Proliferation and Apoptotic Death in Bovine Follicular Cells
Authors: Jewgenow K , Heerdegen B , M K , üller . 
Published: October 2007

ContentsThis study was conducted to characterize the degree of proliferation and atresia in mid‐luteal and follicular phase bovine follicles of different size containing morphologically good quality cumulus oocyte complexes. Follicular cell proliferation was estimated by enzyme immunoassay for tissue polypeptide specific antigen and atresia by an enzyme immunoassay for apoptosis. The relationship between follicular apoptosis and proliferation was inverse in the mid‐luteal stage ovaries, whereas in follicular stage ovaries the tissue polypeptide specific antigen was increased in follicles with the highest degree of apoptosis. Follicular stage follicles showed a higher degree of apoptosis in comparison with the mid‐luteal stage. This was caused by an increased apoptosis in follicles with less than 3 mm and greater than 5 mm diameter. In conclusion, the data indicate a sophisticated interplay between follicular growth and atresia in dependence of oestrous stage and follicular size.
Control of Ovarian Activity in Llamas (Lama glama) with Medroxyprogesterone Acetate
Authors: Aba M.A. , Quiroga M.A. , Auza N. , Forsberg M. , Kindahl H. 
Published: October 2007

ContentsProgesterone and progestogens have proved to be effective in controlling follicle development and synchronization of ovarian activity in different species. In this study, vaginal sponges containing 120 mg medroxyprogesterone acetate were used to synchronize ovarian activity in llamas and to predict the time when a mature follicle will be present. Plasma concentrations of oestradiol‐17β and progesterone were measured to determine follicle and corpus luteum development. The sponges were kept in the vagina for 9 days. Six days after sponge withdrawal, ovulation was induced by either GnRH injection (n = 4), mating with a vasectomized male (n = 8) or mating with an intact male (n = 10). Plasma progesterone concentrations varied between animals until day 6 after insertion of the sponges. Thereafter, progesterone levels remained close to the detection limit of the assay until ovulation was induced. The mean oestradiol‐17β plasma concentration reached its lowest value 3–4 days after insertion of the sponges. Thereafter, concentrations increased and reached peak levels at day 6 after withdrawal of the sponges. All animals ovulated and developed a corpus luteum with a normal life span after the ovulatory stimulus. Blood samples were collected frequently after mating to evaluate the endocrine response to copulation. Plasma concentrations of PGF2α metabolite and cortisol increased in parallel after copulation. The metabolite concentrations returned to basal levels 3–4 h after mating whereas the cortisol concentrations remained elevated for about 12 h after copulation The luteinizing hormone secretory pattern resembled that reported when llamas with a mature ovulatory follicle were mated. In conclusion, the protocol evaluated in this study was shown to be useful for the synchronization of ovarian activity and for predicting the time when an ovulatory follicle will be present in llamas.
Studies on the Lactic Dehydrogenase (LDH) during Development
Authors: Matsuoka Satoru 
Published: October 2007

SummaryThe LDH activities of serum and homogenated rat heart, kidney and liver during development were measured by the method of Wrbblewski and the isozyme patterns observed by the agar gel electrophoresis and the following results were obtained.1) LDH activityIn rat serum, no significant differences were indicated among the activities in six age groups during development.The measurement of the activity in these homogenated tissues revealed the highest in heart and the fairly high titers in kidney and liver. In all tissues, LDH activity changed during development showing the relatively low titer in heart and kidney and the higher in liver in G4.2) LDH isozymeThe LDH isozyme patterns of each tissues gradually changed during development by different course observed in the human tissues.LDH‐5 was the most principal isozyme in rat serum and LDH‐4 and LDH‐5 were consistently dominant through the whole life.In homogenated heart, LDH‐3 was dominant in the early life period and the LDH isozyme patterns changed gradually to H type predominance.In kidney, LDH‐5 was the major isozyme in the early life period and isozyme patterns gradually alterated to H type predominance showing relatively dominant in LDH‐5 ares.In rat liver, LDH‐5 was also the major isozyme in the early life period and difinitely kept the predominance to the adult period.3) The mechanisms of these phenomena were discussed.
Biochemical Characteristics in the Lung of Vitamin Deficiency
Authors: Mino Makoto 
Published: October 2007

SummaryChanges in total phospholipids, fatty acid composition and sulfhydryl groups were examined in the lungs of vitamin E deficient mice and rats.A decrease in the total phospholipid level was observed in the supernatant as well as in the sediment fractions of the lungs of vitamin E deficient animals.Most specific changes in fatty acid composition, observed in the lungs of vitamin E deficiency were the decrease of linoleic acid in the supernatant as well as the sediment fractions. Similar changes in the fatty acid composition were observed in the erythrocyte ghost of vitamin E deficient mice.The sulfhydryl contents based on tissue protein were slightly low, but not significant, in the lungs of vitamin E depleted animals.
Studies on Alkaline Phosphatase during Development Part I: Changes of alkaline phosphatase activity and isozyme pattern in serum, liver, bone, small intestine and kidney of rats during development
Authors: Mimura Tomohiko 
Published: October 2007

SummaryThe author tried to clarify the changes of alkaline phosphatase activity and isozyme patterns in serum, liver, bone, kidney and small intestine of rats during development and organ origins of serum alkaline phosphatase appearing along with growing.Normal 30 rats were divided into 6 groups according to age and the alkaline phosphatase activity in serum and each organ was measured respectively. Alkaline phosphatase isozymes were separated by agar gel electrophoresis and the decreasing rate of alkaline phosphatase activity was measured by heat stability test.1) Organ alkaline phosphatase activity in rats was the highest in small intestine followed by the kidney and bone, and the lowest in the liver. In the change of alkaline phosphatase activity in organs according to age, no remarkable fluctuations were noted in the small intestine and the liver. In the kidney, a transient rise was noted in group 4. In the bone, the highest value was o btained in group 1 with gradual decrease, a slight rise was noted in group 5 and the lowest value in group 6.2) Serum alkaline phosphatase activity in rats was 2 to 3 times higher than that in human. The higher activity was noted in group 1 and 5, and the lowest in group 6.3) In alkaline phosphatase isozyme patterns in organs, the liver showed 2 bands and the other one band, and these isozymes showed different electrophoretic migration rates respectivery. The alkaline phosphatase isozyme pattern of liver consisted of two bands (L‐Ap1 & L‐Ap2), and showed characteristic changes during development.4) The isozyme pattern of serum consisted of two bands (S‐Ap1 & S‐Ap2)showed unique change during development.5) Heat stability test of serum and the organ alkaline phosphatase revealed that the order of the stability was as follows; L‐Ap1 in the liver, the small intestine alkaline phosphatase, the kidney alkaline phosphatase, S‐Ap2 in serum, the bone alkaline phosphatase, S‐Ap2 in serum, and L‐Ap2 in liver.6) These findings suggested that S‐Ap1 in the serum was originated from the bone, and S‐Ap2 in the serum was the mixture of small intestinal alkaline phosphatase and L‐Ap2 of the liver. To sum up, the serum alkaline phosphatase of the rats in young age appeared to originated from bone and in the relatively old age group, other serum alkaline phosphatase originating from small intestine and or liver appeared to be added.
A Case of Hypothyroidism Probably Caused by 131I Transmitted in Maternal Milk
Authors: Nakajima Hironori , Sasaki Nozomu , Kadomura Norio , Niimi Hiroo 
Published: October 2007

SummaryWhen the patient was 1–2 years of age, the mother was treated with a total dose of 19 mCi 131I for hyperthyroidism. Lactation continued during treatment. The patient became hypothyroid 2–3 years later, characterized by a markedly shortened stature. Treatment with desicated thyroid resulted in a euthyroid status and improvement of the short stature.
Prospect of the Monitoring System for Congenital Disorders in Japan
Authors: Yamamura Y. 
Published: October 2007

AbstractThe study of the monitoring system for congenital disorders in Japan is still in its early stage. In reviewing the short history of the study, we can notice some serious problems, i.e. the lack of clear definition, inadequate selection, and unreasonable classification of congenital disorders to be monitored. With this in mind, an extensive program of the monitoring system for congenital disorders was organized in 1979 by the Ministry of Health and Welfare. It is in accord with a global view to establish a new monitoring system, getting an accurate picture of congenital disorders of the Japanese people and having a quick response to the new flows, and setting up a monitoring center.
Full Participation and Equality in Japanease Education Today and in Future1
Authors: Ishikawa Norihiko 
Published: October 2007

AbstractThe National Law for Special Education in 1979 unfortunately enforces the segregation of the handicapped children. Many new private trials, however, have shown that integration is very important for the handicapped children. They also have proved that integration is much easier than has been thought. Some of these trials are reviewed with our case reports and the new approach requested is discussed.
Secretin Secretion in Infancy and Childhood. Part 2. Secretin Secretion in Various Diseases
Authors: Yamamoto Koichi 
Published: October 2007

AbstractTo elucidate secretin secretion in children with gastrointestinal diseases or diabetes mellitus, the present author determined the plasma secretin levels following test‐meals or after an insulin injection, in 56 cases of infants and children with viral winter diarrhea, congenital pyloric stenosis, gastric or duodenal ulcer, superior mesenteric artery syndrome, or juvenile diabetes mellitus.In addition, the effect of glucocorticoid on secretin secretion was also investigated in children with anaphylactoid purpura and in young rats.1. In controls, the plasma secretin level significantly rose after milk or beef broth feeding and showed no significant elevation following glucose administration or intravenous insulin injection2. In viral winter diarrhea, the plasma secretin level did not significantly rise after milk feeding and both the levels after fasting and milk feeding were significantly lower than those in the controls.3. In congenital hypertrophic pyloric stenosis, the plasma secretin level significantly rose after milk feeding, but the peak level was significantly lower than that in the controls.4. In duodenal ulcer, the plasma secretin levels significantly rose after beef broth feeding or intravenous insulin injection and the peak levels were significantly higher than those in the controls.In gastric ulcer, the peak levels obtained were fairly high in both tests.5. In superior mesenteric artery syndrome, the plasma secretin levels after fasting and beef broth feeding were significantly lower than those in the controls. The secretin secretion was not affected by changing position from upright to prone.6. In juvenile diabetes mellitus, the plasma secretin level significantly rose after glucose administration and both the levels after fasting and glucose administration were significantly higher than those in the controls.7. The stimulating effect of glucocorticoid on secretin secretion was apparently demonstrated in young rats and in children with anaphylactoid purpura.The obtained data suggested that in various diseases of infants and children the secretin secretion showed the respective patterns and consequently the secretions of the pancreatic juice and the bile associated with food digestion might be different respectively.
Subclinical Hypothyroidism in a Case of Metastatic Thyroid Cancer
Authors: Kohno Hitoshi , Okabe Yasufumi , Mizuno Yuhji , Hino Keiko , Fujimoto Takeo , Gohya Nagahide 
Published: October 2007

AbstractSubclinical hypothyroidism in a case of papillary adenocarcinoma of the thyroid with lung metastases is reported. The patient at first seemed to be euthyroid with normal concentrations of serum triiodothyronine, thyroxine and thyrotropin (TSH). However, TSH response to thyrotropin‐releasing hormone was above the normal range before thyroidectomy. These data suggested that the patient was in mild hypothyroidism and under prolonged stimulation of TSH. This observation may be the first to be reported in childhood thyroid cancer.
Application of a Live Varicella Vaccine to the Immunosuppressive Children and the Incibence of a Case of Herpes Zoster after Vaccination
Authors: Minamitani Mikio , Numajiri Shinobu , Imada Yoshio , Takayama Michiko , Oya Akira 
Published: October 2007

AbstractA live varicella vaccine was applied to 13 susceptible children to varicella virus. Three were receiving steroid therapy, and one of them was treated with both steroid and anticancer drugs. Immunosuppressive therapy and anticancer medication were not suspended before and after vaccination. Serological responses were observed in 11 of 13 vaccinated children by fluorescent antibody to membrane antigen (FAMA) test 6 to 7 weeks after Vaccination. Mild rash appeared as only a clinical reaction in 3 of the vaccinated children. However, 3 and a half months later, a vaccinee with acute myeloblastic leukemia, developed herpes zoster. Our observation suggested a possibility of contracting zoster in immune compromised hosts after vaccination with a live varicella vaccine.
Inhibition of Lymphocyte Colony Formation in vitro by Culture Supernatant of Human Cord Blood Mononuclear Cells
Authors: Shibasaki Masanao , Nemoto Hirobumi , Suzuki Shigeyoshi , Kuroume Takayoshi 
Published: October 2007

AbstractCulture supernatants from unstimulated cord blood mononuclear cells (CBM) inhibited the proliferation of lymphoid cells of adult and newborns in semisolid cultures and the T‐cell‐rich fraction was found to have mainly this suppressor activity. On the 3rd to 5th day of incubation of CBM, maximal inhibitory activity was found to occur. In contrast, suppressor activity of the culture supernatant was effectively abrogated, when the CBM were incubated for 48 hr with phytohemagglutinin‐P (PHA‐P), concanavalin A (Con A) or protein A added at the concentration of 50 ug/ml or with 1 /ug/m/ of poke‐weed mitogen (PWM) and returned to a mitogen‐free medium for another 48 hr.
Further Study on the Suspected Case of Tuftsin Deficiency
Authors: Nemoto Norio , Ogasawara Junko , Inada Katsuya , Hirata Michimasa , Yoshida Masao 
Published: October 2007

AbstractA patient whose tuftsin‐activities of serum and PMN had been found to be deficient was reported in 1975 by us. After the elapse of 4.5—5.5 years, tuftsin‐activity in addition to chemotactic, bactericidal and NBT‐reducing activities of the the patient's PMN were investigated. The tuftsin and bactericidal activities against staphylococcus of the patient's PMN fell within the normal range. However, as to the NBT‐test and chemotaxis, the patient and her sister who had suffered from similar symptoms in infancy were found to have lower activities. In addition, the serum IgE level of the patient was slightly higher.It is obscure as to why the patient's tuftsin‐activity recovered to a normal level, although she has not received γ‐globulin therapy for 5.5 years.It is conceivable that the patient suffers from a dysfunction of PMN, but it is impossible at the present time to make a definite diagnosis.
“Pulse” Methylprednisolone Therapy in the Treatment of Idiopathic Thrombocytopenic Purpura: A preliminary Report
Authors: Takayama Yoichi , Ouchi Toru , Iwai Asayuki , Iwai Tsuyako , Ninomiya Tsuneo , Miyao Masuhide , Yoshida Shigehiko 
Published: October 2007

AbstractPulse‐mode methylprednisolone therapy was instituted in the treatment of idiopathic thrombocytopenic purpura (ITP), both acute and chronic forms. This regimen induced a rapid increase in the platelet count in a short time and was less toxic than the conventional mode of therapy, i.e. oral steroid hormone administration. This regimen is of value in the management of patients with ITP who are at the substantial risk of fatal hemorrhage. And this is also useful as a short‐term presplenectomy preparation or as a “medical splenectomy” program. Impressive responses in our experience encouraged us to evaluate this mode of therapy.
Bone Ages and Heights in Congenital Rubella Syndrome
Authors: Ueda Kohji , Tokugawa Ken , Watanabe Noriaki 
Published: October 2007

AbstractEighty‐eight children with congenital rubella syndrome (aged 10 0/12 years to 10 11/12 years) and 73 normal children (aged 9 11/12 years to 11 4/12 years), all born in the Ryukyu Islands of Southern Japan, were assessed for their bone ages and heights. No significancy was noted between the development of the bone ages of the two groups studied, despite a difference between the heights of the two groups.
Etiology of the Mucocutaneous Lymph Node Syndrome, and the Streptococcus Hypothesis
Authors: Yamamoto Takajiro 
Published: October 2007

AbstractThe epidemiology, clinical and laboratory findings, and the progress of the mucocutaneous lymph node syndrome (Kawasaki disease) were analyzed for clue to its etiology. Though there are several controversies against the possibility of Streptococcus hemolyticus in the etiology, such as failure of getting regularly the agent by culture from patients and inefficacy of antibiotics, close similarity in symptomatology and common possession of cardiovascular complications by the syndrome and streptococcal infection are very suggestive of the possibility. Suppurative or septic forms of streptococcal infections in infants and young children, which were seen at the time when antibiotics were not used, showed nearly identical curves of age incidence to that of this syndrome. Sero‐epidemiological study by Kusama et al., which has revealed the percentage distribution curve by age of “ASO negative subjects” is also very similar to the age incidence curve of this syndrome, seems to offer another support to the hypothesis. The well documented cross reactivity between streptococcus cell wall and cardiac muscle or arteriolar smooth muscle fibers of mammalian, seems to be another support to the theory.
Isolation of Chlamydia Trachomatis from Japanese Infancts with Pneumonia
Authors: Numazaki Kei , Chiba Shunzo , Yamanaka Tatsuru , Umetsu Masao , Nakao Tooru 
Published: October 2007

AbstractChlamydia trachomatis (C.trachomatis) is now being recognized as an important pathogen of distinctive pneumonia in infants. However, little is known about the pathogenic role of C.trachomatis in Japan as well as in many other Asian countries.Nasopharyngeal swabs were collected from 87 infants with pneumonia. Conjunctival swabs were also collected from 21 babies with neonatal conjunctivitis. For the control 55 age‐matched patients without respiratory tract infection were also examined. Clinical specimens were inoculated onto DEAE‐Dextran‐treated HeLa 229 cells and cycloheximide‐treated McCoy cells.Of 87 infants with pneumonia, 22 (25.3%) were positive for C.trachomatis, whereas 3 (5.5%) out of 55 control subjects were found to be positive. Of 21 babies with neonatal conjunctivitis, 6 (28.6%) were positive.Our data suggest that C.trachomatis plays an important role in pneumonia and conjunctivitis in Japanese infants.