bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molar incisor hypomineralization: a survey of members of the Australian and New Zealand Society of Paediatric Dentistry
Authors: Crombie FA , Manton DJ , Weerheijm KL , Kilpatrick NM 
Published: May 2008

AbstractBackground:  Worldwide, molar incisor hypomineralization (MIH) affects a substantial number of children and impacts greatly on treatment need and dental anxiety, yet there is little information regarding its prevalence, aetiology, presentation and management. The aims of this survey were to assess awareness and perceptions of the Australian paediatric dental community concerning MIH, and to describe current treatment strategies.Methods:  A questionnaire, based upon a previous European study, was sent to all Australian members of the Australian and New Zealand Society of Paediatric Dentistry. The questionnaire sought information on clinical experience of MIH, knowledge of prevalence, aetiology and contemporary management strategies for MIH.Results:  One hundred and thirty useable responses were received (58.8 per cent response rate) of which 36 were paediatric dentists, 6 paediatric dentistry postgraduate students, 59 general dentists, 14 dental therapists and 14 specialists in other fields. Most (98.5 per cent) respondents were familiar with MIH and encountered it in their practice. The majority (73.1 per cent) estimated that MIH occurred in between 5 to 25 per cent of their clinical practice and almost all (96.9 per cent) considered it to be a clinical problem. Only 16.9 per cent of respondents were aware of existing prevalence data and 96.9 per cent valued investigating the prevalence of MIH. No consensus existed regarding the aetiology of MIH or its restorative management. Paediatric dentists used preformed crowns significantly more than non‐specialists, however glass ionomer cements were popular with all groups.Conclusions:  MIH is a well recognized and widely encountered clinical condition. MIH presents several clinical problems and is worthy of further investigation. Currently, no consistent clinical management strategies are utilized.
Microleakage of composite resin restorations in cervical cavities prepared by Er,Cr:YSGG laser radiation
Authors: Shahabi S , Ebrahimpour L , Walsh LJ 
Published: May 2008

AbstractBackground:  Evaluation of microleakage is important for assessing the success of new methods for surface preparation and new adhesive restorative materials. The aim of this laboratory study was to assess microleakage at the margins of composite restorations in Er,Cr:YSGG laser prepared cavities on the cervical aspects of teeth by means of dye penetration, and compare this with conventionally prepared and conditioned cavities.Methods:  Class V cavities were produced on sound extracted human teeth, which had been assigned randomly to one of three groups (N = 10 each), as follows: Group 1 – prepared using a diamond cylindrical bur and then treated with 37% phosphoric acid; Group 2 – irradiated with an Er,Cr:YSGG laser (Biolase Waterlase) and then treated with 37% phosphoric acid; Group 3 – irradiated only with the laser. After application of bonding agent (Excite, Ivoclar Vivadent), all cavities were restored with composite resin (Heliomolar). After polishing the restorations, the teeth were thermocycled from 5–50°C for 500 cycles. Dye leakage was assessed after immersion in methylene blue, by examining longitudinal sections in a stereomicroscope at ×30 magnification.Results:  The extent of dye penetration was lowest in the laser only group (Group 3). Penetration of dye to dentine and axial walls occurred in 80 per cent of conventionally prepared (bur + acid) specimens, but in the laser group, dye penetration to the axial wall occurred in only 30 per cent of cases. There was a strong statistical association between treatment group and the distribution of microleakage scores (Chi‐square, P = 0.0023).Conclusions:  For Class V cavities, with the adhesive materials employed, higher microleakage occurs with phosphoric acid etching of bur‐ or laser‐cut surfaces, than with the surface created by use of the laser alone without additional conditioning.
Cohomology of topological groups with applications to the Weil group
Authors: Flach M. 
Published: May 2008

AbstractWe establish various properties of the definition of cohomology of topological groups given by Grothendieck, Artin and Verdier in SGA4, including a Hochschild–Serre spectral sequence and a continuity theorem for compact groups. We use these properties to compute the cohomology of the Weil group of a totally imaginary field, and of the Weil-étale topology of a number ring recently introduced by Lichtenbaum (both with integer coefficients).
Petri map for rank two bundles with canonical determinant
Authors: Teixidor i Bigas Montserrat 
Published: May 2008

AbstractWe prove the Bertram–Feinberg–Mukai conjecture for a generic curve C of genus g and a semistable vector bundle E of rank two and determinant K on C, namely we prove the injectivity of the Petri-canonical map S2(H0(E))→H0(S2(E)).
Data mining for miRNAs and their targets in the Triticeae
Authors: Dryanova Ani , Zakharov Arseny , Gulick Patrick J. 
Published: May 2008

MicroRNAs (miRNAs) and the mRNA targets of miRNAs were identified by sequence complementarity within a DNA sequence database for species of the Triticeae. Data screening identified 28 miRNA precursor sequences from 15 miRNA families that contained conserved mature miRNA sequences within predicted stem-loop structures. In addition, the identification of 337 target sequences among Triticeae genes provided further evidence of the existence of 26 miRNA families in the cereals. MicroRNA targets included genes that are homologous to known targets in diverse model species as well as novel targets. MicroRNA precursors and targets were identified in 10 related species, though the great majority of them were identified in bread wheat, Triticum aestivum , and barley, Hordeum vulgare , the two species with the largest EST data sets among the Triticeae.
MISS RATIO IMPROVEMENT FOR REAL-TIME APPLICATIONS USING FRAGMENTATION-AWARE PLACEMENT
Authors: ELFARAG AHMED A. , El-BOGHDADI HATEM M. , SHAHEEN SAMIR I. 
Published: May 2008

Partially reconfigurable field-programmable gate arrays (FPGAs) allow parts of the chip to be configured at runtime where each part could hold an independent task. Online placement of these tasks result in area fragmentation leading to poor utilization of chip resources. In this paper, we propose a new metric for measuring area fragmentation. The new fragmentation metric gives an indication to the continuity of the occupied (or free) space and not the amount of occupied space. We show how this metric can be extended for multidimensional structures. We also show how this metric can be computed efficiently at runtime. Next, we use this measure during online placement of tasks on FPGAs, such that the chip fragmentation is reduced. Our results show improvement of chip utilization when using this fragmentation aware placement method over other placement methods with well-known bottom left, first fit, and best-fit placement strategies. In real-time environment, we achieve an improvement in miss ratio when using the fragmentation-aware placement over the bottom left placement strategy.
Secrecy and "Two-Level Games" in the Oslo Accord: What the Primary Sources Tell Us
Authors: Lieberfeld Daniel 
Published: May 2008

AbstractThe publication of memoirs by most of the central participants in negotiations leading to the 1993 Israeli-PLO Oslo accord presents an opportunity to reassess their motives for entering the negotiation process and their reasons for electing to keep the talks secret. Among other insights, the first-hand accounts provide empirical evidence of "two-level games" during the negotiations. The article highlights the utility of Robert Putnam's concepts in understanding the negotiations in Oslo and to the failure to implement final-status talks as envisioned in the accord.
Tropical Indian Ocean Basin Warming and East Asian Summer Monsoon: A Multiple AGCM Study
Authors: Li Shuanglin , Lu Jian , Huang Gang , Hu Kaiming 
Published: May 2008

Abstract
A basin-scale warming is the leading mode of tropical Indian Ocean sea surface temperature (SST) variability on interannual time scales, and it is also the prominent feature of the interdecadal SST trend in recent decades. The influence of the warming on the East Asian summer monsoon (EASM) is investigated through ensemble experiments of several atmospheric general circulation models (AGCMs). The results from five AGCMs consistently suggest that near the surface, the Indian Ocean warming forces an anticyclonic anomaly over the subtropical western Pacific, intensifying the southwesterly winds to East China; and in the upper troposphere, it forces a Gill-type response with the intensified South Asian high, both favoring the enhancement of the EASM. These processes are argued to contribute to the stronger EASM during the summer following the peak of El Niño than monsoons in other years. These model results also suggest that tropical Indian Ocean warming may not have a causal relationship to the synchronous weakening of EASM on interdecadal time scales.
A Place to Be Celebrated and Understood
Authors: Noble Kathleen D. , Childers Sarah A. , Vaughan Robert C. 
Published: May 2008

This study surveyed 181 parents of students enrolled in one of two early entrance programs at the University of Washington: the Early Entrance Program for students who entered before age 15 and the University of Washington Academy for Young Scholars for students who matriculated after Grade 10. The purpose was to understand parents' perspectives on the early entrance experience, including reasons for choosing early entrance, satisfaction with their children's program, perceived advantages and disadvantages of early entrance, and the effect of early entrance on family relationships. Ninety-five parents participated, with the majority reporting great satisfaction with multiple aspects of their and their children's experience. Early Entrance Program parents expressed a slightly higher degree of satisfaction than did Academy parents. This study suggests that early university entrance is an attractive option for parents willing to follow the lead of their talented and ambitious adolescents. Putting the Research to Use: Early entrance programs are attractive options for parents of academically advanced students who have outpaced available secondary programs. These programs are not inevitably initially successful; however, as our study demonstrates, they benefit greatly from ongoing program evaluation and analysis. Key components of effective early entrance programs include intensive advising, community building, thoughtful selection of students, active engagement by students and faculty, and a welcoming college or university environment. Careful attention to the informational needs of parents is also important, particularly about the social and emotional transitions that they and their children are likely to experience. The University of Washington has demonstrated with two programs that early university entrance is prized by students, parents, university officials, and state education agencies. These and similar programs might well be replicated in other areas around the country given active partnerships among all concerned parties.
Integrin αE(CD103)β7 influences cellular shape and motility in a ligand-dependent fashion
Authors: Schlickum Stephanie , Sennefelder Helga , Friedrich Mike , Harms Gregory , Lohse Martin J. , Kilshaw Peter , Schön Michael P. 
Published: May 2008

AbstractWhile the extravasation cascade of lymphocytes is well characterized, data on their intraepithelial positioning and morphology are scant. However, the latter process is presumably crucial for many immune functions. Integrin αE(CD103)β7 has previously been implicated in epithelial retention of some T cells through binding to E-cadherin. Our current data suggest that αE(CD103)β7 also determines shape and motility of some lymphocytes. Time-lapse microscopy showed that wild-type αE(CD103)β7 conferred the ability to form cell protrusions/filopodia and to move in an amoeboid fashion on E-cadherin, an activity that was abrogated by αE(CD103)β7-directed antibodies or cytochalasin D. The αE-dependent motility was further increased (P < .001) when point-mutated αE(CD103) locked in a constitutively active conformation was expressed. Moreover, different yellow fluorescent protein–coupled αE(CD103) species demonstrated that the number and length of filopodia extended toward purified E-cadherin, cocultured keratinocytes, cryostat-cut skin sections, or epidermal sheets depended on functional αE(CD103). The in vivo relevance of these findings was demonstrated by wild-type dendritic epidermal T cells (DETCs), which showed significantly more dendrites and spanned larger epidermal areas as compared with DETCs of αE(CD103)-deficient mice (P < .001). Thus, integrin αE(CD103)β7 is not only involved in epithelial retention, but also in shaping and proper intraepithelial morphogenesis of some leukocytes.
Failure to achieve a major cytogenetic response by 12 months defines inadequate response in patients receiving nilotinib or dasatinib as second or subsequent line therapy for chronic myeloid leukemia
Authors: Tam Constantine S. , Kantarjian Hagop , Garcia-Manero Guillermo , Borthakur Gautam , O'Brien Susan , Ravandi Farhad , Shan Jenny , Cortes Jorge 
Published: May 2008

Abstract
To determine when patients with incomplete responses on second-line tyrosine kinase inhibitor (2TKI) therapy should consider alternative treatment, we analyzed the outcome of 113 patients receiving nilotinib (n = 43) or dasatinib (n = 70) after imatinib failure. After 12 months of 2TKI therapy, patients achieving a major cytogenetic response (12MMCyR) had a significant survival advantage over patients in minor cytogenetic response or complete hematologic response, with a projected one-year survival of 97% and 84% respectively (P = .02). Projected 1-year progression to hematologic failure, accelerated phase, or blast phase was also significantly different (3% vs 17%, P = .003). Early cytogenetic response was strongly predictive of achievement of 12MMCyR, with less than 10% of patients showing no cytogenetic response at 3 to 6 months eventually attaining the target of 12MMCyR. These results suggest that patients receiving 2TKI with no cytogenetic response at 3 to 6 months should be considered for alternative therapies.
Thalidomide arm of Total Therapy 2 improves complete remission duration and survival in myeloma patients with metaphase cytogenetic abnormalities
Authors: Barlogie Bart , Pineda-Roman Mauricio , van Rhee Frits , Haessler Jeff , Anaissie Elias , Hollmig Klaus , Alsayed Yazan , Waheed Sarah , Petty Nathan , Epstein Joshua , Shaughnessy John D. , Tricot Guido , Zangari Maurizio , Zeldis Jerome , Barer Sol , Crowley John 
Published: May 2008

AbstractTotal Therapy 2 examined the clinical benefit of adding thalidomide up-front to a tandem transplant regimen for newly diagnosed patients with multiple myeloma. When initially reported with a median follow-up of 42 months, complete response rate and event-free survival were superior among the 323 patients randomized to thalidomide, whereas overall survival was indistinguishable from that of the 345 patients treated on the control arm. With further follow-up currently at a median of 72 months, survival plots segregated 5 years after initiation of therapy in favor of thalidomide (P = .09), reaching statistical significance for the one third of patients exhibiting cytogenetic abnormalities (CAs; P = .02), a well-recognized adverse prognostic feature. The duration of complete remission was also superior in the cohort presenting with CAs such that, at 7 years from onset of complete remission, 45% remained relapse-free as opposed to 20% on the control arm (P = .05). These observations were confirmed when examined by multivariate analysis demonstrating that thalidomide reduced the hazard of death by 41% among patients with CA-positive disease (P = .008). Because two thirds of patients without CAs have remained alive at 7 years, the presently emerging separation in favor of thalidomide may eventually reach statistical significance as well.
MicroRNA-encoding long non-coding RNAs
Authors: He Shunmin , Su Hua , Liu Changning , Skogerbø Geir , He Housheng , He Dandan , Zhu Xiaopeng , Liu Tao , Zhao Yi , Chen Runsheng 
Published: May 2008

Abstract

Background
Recent analysis of the mouse transcriptional data has revealed the existence of ~34,000 messenger-like non-coding RNAs (ml-ncRNAs). Whereas the functional properties of these ml-ncRNAs are beginning to be unravelled, no functional information is available for the large majority of these transcripts.

Results
A few ml-ncRNA have been shown to have genomic loci that overlap with microRNA loci, leading us to suspect that a fraction of ml-ncRNA may encode microRNAs. We therefore developed an algorithm (PriMir) for specifically detecting potential microRNA-encoding transcripts in the entire set of 34,030 mouse full-length ml-ncRNAs. In combination with mouse-rat sequence conservation, this algorithm detected 97 (80 of them were novel) strong miRNA-encoding candidates, and for 52 of these we obtained experimental evidence for the existence of their corresponding mature microRNA by microarray and stem-loop RT-PCR. Sequence analysis of the microRNA-encoding RNAs revealed an internal motif, whose presence correlates strongly (R
2 = 0.9, P-value = 2.2 × 10-16) with the occurrence of stem-loops with characteristics of known pre-miRNAs, indicating the presence of a larger number microRNA-encoding RNAs (from 300 up to 800) in the ml-ncRNAs population.

Conclusion
Our work highlights a unique group of ml-ncRNAs and offers clues to their functions.
A novel resource for genomics of Triticeae: BAC library specific for the short arm of rye (Secale cereale L.) chromosome 1R (1RS)
Authors: Šimková Hana , Šafář Jan , Suchánková Pavla , Kovářová Pavlína , Bartoš Jan , Kubaláková Marie , Janda Jaroslav , Číhalíková Jarmila , Mago Rohit , Lelley Tamas , Doležel Jaroslav 
Published: May 2008

Abstract

Background
Genomics of rye (Secale cereale L.) is impeded by its large nuclear genome (1C~7,900 Mbp) with prevalence of DNA repeats (> 90%). An attractive possibility is to dissect the genome to small parts after flow sorting particular chromosomes and chromosome arms. To test this approach, we have chosen 1RS chromosome arm, which represents only 5.6% of the total rye genome. The 1RS arm is an attractive target as it carries many important genes and because it became part of the wheat gene pool as the 1BL.1RS translocation.

Results
We demonstrate that it is possible to sort 1RS arm from wheat-rye ditelosomic addition line. Using this approach, we isolated over 10 million of 1RS arms using flow sorting and used their DNA to construct a 1RS-specific BAC library, which comprises 103,680 clones with average insert size of 73 kb. The library comprises two sublibraries constructed using Hin dIII and Eco RI and provides a deep coverage of about 14-fold of the 1RS arm (442 Mbp). We present preliminary results obtained during positional cloning of the stem rust resistance gene SrR, which confirm a potential of the library to speed up isolation of agronomically important genes by map-based cloning.

Conclusion
We present a strategy that enables sorting short arms of several chromosomes of rye. Using flow-sorted chromosomes, we have constructed a deep coverage BAC library specific for the short arm of chromosome 1R (1RS). This is the first subgenomic BAC library available for rye and we demonstrate its potential for positional gene cloning. We expect that the library will facilitate development of a physical contig map of 1RS and comparative genomics of the homoeologous chromosome group 1 of wheat, barley and rye.
Simultaneous gene expression profiling in human macrophages infected with Leishmania major parasites using SAGE
Authors: Guerfali Fatma Z , Laouini Dhafer , Guizani-Tabbane Lamia , Ottones Florence , Ben-Aissa Khadija , Benkahla Alia , Manchon Laurent , Piquemal David , Smandi Sondos , Mghirbi Ons , Commes Thérèse , Marti Jacques , Dellagi Koussay 
Published: May 2008

Abstract

Background

Leishmania (L) are intracellular protozoan parasites that are able to survive and replicate within the harsh and potentially hostile phagolysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MΦ) striving to eliminate the pathogen and the parasite struggling for its own survival.
To investigate this host-parasite conflict at the transcriptional level, in the context of monocyte-derived human MΦs (MDM) infection by L. major metacyclic promastigotes, the quantitative technique of serial analysis of gene expression (SAGE) was used.

Results
After extracting mRNA from resting human MΦs, Leishmania-infected human MΦs and L. major parasites, three SAGE libraries were constructed and sequenced generating up to 28,173; 57,514 and 33,906 tags respectively (corresponding to 12,946; 23,442 and 9,530 unique tags). Using computational data analysis and direct comparison to 357,888 publicly available experimental human tags, the parasite and the host cell transcriptomes were then simultaneously characterized from the mixed cellular extract, confidently discriminating host from parasite transcripts. This procedure led us to reliably assign 3,814 tags to MΦs' and 3,666 tags to L. major parasites transcripts. We focused on these, showing significant changes in their expression that are likely to be relevant to the pathogenesis of parasite infection: (i) human MΦs genes, belonging to key immune response proteins (e.g., IFNγ pathway, S100 and chemokine families) and (ii) a group of Leishmania genes showing a preferential expression at the parasite's intra-cellular developing stage.

Conclusion
Dual SAGE transcriptome analysis provided a useful, powerful and accurate approach to discriminating genes of human or parasitic origin in Leishmania-infected human MΦs. The findings presented in this work suggest that the Leishmania parasite modulates key transcripts in human MΦs that may be beneficial for its establishment and survival. Furthermore, these results provide an overview of gene expression at two developmental stages of the parasite, namely metacyclic promastigotes and intracellular amastigotes and indicate a broad difference between their transcriptomic profiles. Finally, our reported set of expressed genes will be useful in future rounds of data mining and gene annotation.