bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vitro Polymerization of Wheat Glutenin Subunits with Inorganic Oxidizing Agents. I. Comparison of Single‐Step and Stepwise Oxidations of High Molecular Weight Glutenin Subunits
Authors: Veraverbeke Wim S. , Larroque Oscar R. , Békés Frank , Delcour Jan A. 
Published: June 2007

ABSTRACTHigh molecular weight glutenin subunits (HMW‐GS) were isolated from wheat flour and polymerized in vitro at pH 3.0 with different oxidizing agents (KBrO3, KIO3, H2O2). An oxidation protocol with single addition of oxidant (single‐step oxidation) was compared with a set‐up in which the oxidant was added in multiple steps (stepwise oxidation). Changes in size distribution were evaluated with size‐exclusion HPLC, multilayer SDS‐PAGE, and flow‐field flow fractionation (flow‐FFF). Flow‐FFF is particularly suitable for measuring changes in glutenin size in the very high size ranges. In order of increasing sizes of the resulting polymers, the different oxidizing agents could be ranked as KBrO3 < KIO3 < H2O2. However, none of the oxidation conditions allowed for a complete polymerization of HMW‐GS. Interestingly, it was found that high concentrations of KIO3 negatively affect the degree of polymerization. A similar observation was not made with KBrO3 or H2O2. SDS‐PAGE showed that y‐type HMW‐GS particularly failed to incorporate in glutenin polymers. Simultaneously, these HMW‐GS displayed higher mobilities on SDS‐PAGE that can be ascribed to the formation of intrachain SS bonds. Possible explanations for the incomplete polymerization of HMW‐GS are given.
Observations on the α‐Amylolysis Pattern of Some Waxy Maize Starches from Inbred Line Ia453
Authors: Bertoft Eric , Boyer Charles , Manelius Robin , Åvall Ann‐Kristin 
Published: June 2007

ABSTRACTMaize starches of the endosperm mutants waxy (wx), dull:waxy (duwx), and amylose‐extender:dull:waxy (aeduwx) from inbred line Ia453 lack amylose. However, in addition to high molecular weight (HMW) amylopectin, the duwx and aeduwx starches contained 40 and 80%, respectively, intermediate branched material of low molecular weight (LMW). As gelatinized, the amylopectin of the wx starch was easily hydrolyzed into small dextrins by the α‐amylase of B. amyloliquefaciens, but components of duwx and aeduwx possessed partial resistance to amylolytic attack. Residual material of intermediate size obtained by a 4‐hr α‐amylolysis could not be separated from LMW dextrins by fractional precipitation in methanol. It is suggested that this material possessed a more regularly branched structure, in which the d‐glucosyl chain segments were too short to allow α‐amylase action. The granular starches of duwx and aeduwx genotypes were initially considerably more resistant than the wx sample to α‐amylase attack. This was possibly due to an altered structure in the amylopectin component or the high content of intermediate material in the former granules.
Hybrid Variability and Effect of Growth Location on Corn Fiber Yields and Corn Fiber Oil Composition
Authors: Singh Vijay , Moreau Robert A. , Haken Armgard E. , Eckhoff Steven R. , Hicks Kevin B. 
Published: June 2007

ABSTRACTThe variability in commercial corn hybrids for corn fiber yields, amounts of extractable oil, and levels of individual and total phytosterol components in corn fiber oil was determined. Also, the effect of growth location on fiber yields, fiber oil content, and the levels of individual and total phytosterol compounds was determined. Significant variation was observed in the commercial hybrids for fiber yield (13.2–16.6%) and fiber oil yield (0.9–2.4%). No significant correlation was observed between fiber and oil yields. Significant variations in the commercial corn hybrids were also observed in the individual phytosterol compounds in corn fiber oil: 2.9–9.2% for ferulate phytosterol esters (FPE); 1.9–4.3% for free phytosterols (St); and 6.5–9.5% for phytosterol fatty acyl esters (St:E). Positive correlations were observed among the three phytosterol compounds in the corn fiber oil (R = 0.75 for FPE and St:E; 0.48 for St:E and St; and 0.68 for FPE and St). The effect of location on dependent variables was also significant. The same hybrids grown at different locations showed a variation (range) of 4.0–17.5% for FPE, 4.9–12.2% for St:E, and 1.95–4.45% for St. Relative ranking of hybrids with respect to phytosterol composition was consistent for almost all of the growth locations.
Breadmaking Quality Estimation By Fast Spectrophotometric Method
Authors: Tosi E. A. , Ré E. D. , Carbone L. , Cuniberti M. 
Published: June 2007

ABSTRACTA fast method to estimate wheat breadmaking quality diminution as a result of excessive thermal treatment during drying is proposed. Proteins that remain in soluble after drying were extracted with a solution of phosphoric acid plus ethanol, and colorimetrically quantified after dyeing with Coomassie Brilliant Blue G and measuring at 595 nm. A decrease in absorbance values indicates a decrease in loaf volume and breadmaking quality. The application of the proposed technique required ≈30 min.
Contribution of Hydrophobic Soluble Gluten Proteins, Fractionated by Hydrophobic Interaction Chromatography in Highly Acetylated Agarose, to Dough Rheological Properties
Authors: Torres P. I. , Vazquez‐Moreno L. , Ledesma‐Osuna A. I. , Medina‐Rodriguez C. 
Published: June 2007

ABSTRACTHydrophobic interaction chromatography with highly acetylated agarose in 1‐mL columns was used to fractionate gliadins and acid‐soluble glutenins. Proteins were eluted in two fractions, the first with acetate buffer (pH 3.6) containing 35% propanol, and the second with Tris buffer in 8M urea. The proportion of eluted protein in the second fraction was called the surface hydrophobicity index. The study included 20 wheat samples of different baking qualities. Multiple regression analysis using the general linear model combined with the stepwise technique was used to relate the surface hydrophobicity index of soluble gluten proteins to specific dough rheological characteristics. Surface hydrophobicity index of gliadins and acetic acid soluble glutenins explained part of the variability of swelling index, extensibility, and work of deformation (dough strength) measured with the alveograph, and part of the farinograph water absorption variability, but showed no relationship to dough mixing characteristics. Hydrophobic soluble gluten proteins fractionated by hydrophobic interaction chromatography (HIC) explained a part of the variability of dough rheological properties.
Glassy State Transition and Rice Drying: Development of a Brown Rice State Diagram
Authors: Perdon A. , Siebenmorgen T. J. , Mauromoustakos A. 
Published: June 2007

ABSTRACTThe effect of moisture content (MC) on the glass transition temperature (Tg) of individual brown rice kernels of Bengal, a medium‐grain cultivar, and Cypress, a long‐grain cultivar, was studied. Three methods were investigated for measuring Tg: differential scanning calorimetry (DSC), thermomechanical analysis (TMA), and dynamic mechanical analysis (DMA). Among these methods, TMA was chosen, because it can also measure changes in the thermal volumetric coefficient (β) of the kernel during glass transition. TMA‐measured Tg at similar MC levels for both cultivars were not significantly different and were combined to generate a brown rice state diagram. Individual kernel Tg for both cultivars increased from 22 to 58°C as MC decreased from 27 to 3% wb. Linear and sigmoid models were derived to relate Tg to MC. The linear model was sufficient to describe the property changes in the MC range encountered during rice drying. Mean β values across both cultivars in the rubbery state was 4.62 × 10‐4/°C and was higher than the mean β value of 0.87 × 10‐4/°C in the glassy state. A hypothetical rice drying process was mapped onto the combined state diagram generated for Bengal and Cypress.
Susceptibility of Waxy Starch Granules to Mechanical Damage
Authors: Bettge A. D. , Giroux M. J. , Morris C. F. 
Published: June 2007

ABSTRACTStarch samples isolated from wheat flour that represented four possible waxy states (0, 1, 2, and 3‐gene waxy) were subjected to crushing loads under both dry and wet conditions. Calibrated loads of 0.5–20 kg were applied to the starch samples and the percentage of damaged granules was visually determined. Under dry crushing conditions, starches containing amylose (0, 1, and 2‐gene waxy) had between 1% (5‐kg load) to 3% (15‐ and 20‐kg load) damaged granules, whereas waxy starch (3‐ gene waxy; <1% amylose) began rupturing at 0.5‐kg load (3.5% damaged granules) and had 13% damaged granules when ≥10‐kg load was applied. Under wet crushing conditions, normal and partial waxy starch (0, 1, and 2‐gene waxy) showed little difference in percentage of damaged granules when compared to the results of dry crushing. Waxy starch (3‐gene waxy), however, showed substantially increased numbers of damaged granules: 12% damaged granules at 0.5‐kg load, rising to 55% damaged granules at 15‐kg load. The results indicate that waxy starch granules are less resistant to mechanical damage than normal starch granules. Furthermore, blends of normal and waxy wheats or wheat flours intended to have a particular amylose‐amylopectin ratio will be a complex system with unique processing and formulation considerations and opportunities.
Scarification and Degermination of Sorghum for Grits Production: Effects of Hybrid and Conditioning
Authors: Suroso Janti , Flores Rolando A. , Boyer John E. 
Published: June 2007

ABSTRACTThree sorghum hybrids were tempered and decorticated with an abrasive‐type mill (scarifier) to produce low‐ash and low‐fat grits. The effects of tempering time and temperature were investigated, and the optimum tempering conditions for obtaining low‐ash and low‐fat grits were found for each sorghum hybrid. The conditions were 3 min at 30°C for bronze sorghum with heteroyellow endosperm, 40 min at 40°C for white sorghum with white endosperm, and 10 min at 20°C for red sorghum with white endosperm. The grits yields were low using the scarifier, hence, another abrasive‐type mill was investigated for improving grits yields. A modified experimental corn decorticator‐degerminator was used to dry‐mill the three sorghum hybrids tempered to the optimum conditions found with the scarifier. The yields were 45.3% grits with 0.23% ash and 0.18% fat for the bronze/heteroyellow hybrid, 49.1% grits with 0.22% ash and 0.36% fat for white/white hybrid, and 44.2% grits with 0.20% ash and 0.22% fat for red/white hybrid. This study showed that grits yields were higher and ash and fat contents were lower when sorghum was processed with the decorticator‐degerminator than with the scarifier under the same optimum conditioning.
Packing Characteristics of Starch Granules
Authors: Willett J. L. 
Published: June 2007

ABSTRACTThe rheological properties of granular materials and dispersions of solid particles in fluids are dependent on the packing characteristics of the particles. Maximum packing fractions (Φm) have been measured for corn, wheat, rice, potato, and amaranth starches, in the dry state and dispersed in either ethanol or hexane, using a tapping method. The observed maximum packing fraction increases with tapping time to a constant value. Values measured for dry starches were lower than those measured in liquids and reflect the effects of granule shape and intergranular friction. Values measured in fluids for potato, corn, and wheat starches were all similar in magnitude, and in the range of values (0.58–0.63) for random loose packing and random close packing of monodisperse spheres. Values for amaranth and rice starches were significantly lower due to agglomeration and clumping of individual granules. Blends of corn and potato starches show a slight enhancement of packing, with some Φm values greater than potato starch, consistent with data for bimodal blends of spheres. Blends of rice and potato starches displayed enhanced packing above ideal mixing but did not exceed the packing fraction of the potato starch. Knowledge of starch packing fractions is required for fundamental understanding of the rheological properties of granular starch‐filled materials and important for predicting processing characteristics.
Use of SDS to Extract Sorghum and Maize Proteins for Free Zone Capillary Electrophoresis (FZCE) Analysis
Authors: Bean S. R. , Hicks C. , Tuinstra M. , Lookhart G. L. 
Published: June 2007

ABSTRACTTwo different extraction methods for extracting sorghum (Sorghum bicolor L. Moench.) storage proteins for free zone capillary electrophoresis (FZCE) analysis were compared. A traditional solvent based on 60% t‐butanol was compared with a pH 10 borate buffer containing the anionic detergent SDS followed by precipitation of nonkafirins using 60% t‐butanol. FZCE analysis of both types of extracts showed identical patterns, despite the fact that the SDS should have given all proteins equal charge‐to‐mass ratios. This methodology was also successfully applied to maize proteins. The use of t‐butanol to precipitate nonkafirins, combined with electrophoresis at low pH, is thought to have removed the SDS from the storage proteins. The SDS extraction procedure produced more stable extracts for FZCE analysis. These extracts could also be used directly for SDS capillary electrophoresis (SDS‐CE) separations. Kafirins from 15 genotypes were extracted with this procedure and analyzed by FZCE and SDS‐CE. Resolution of the kafirins by FZCE was much higher than the SDS‐CE, demonstrating that the kafirin proteins possessed a high level of charge density variability within a relatively small molecular size distribution. Two distinct groups of α‐kafirins could be seen in the FZCE electropherograms.
Alkaline Processing (Nixtamalization) of White Mexican Corn Hybrids for Tortilla Production: Significance of Corn Physicochemical Characteristics and Process Conditions
Authors: Sahai D. , Mua J. P. , Surjewan I. , Buendia M. O. , Rowe M. , Jackson D. S. 
Published: June 2007

ABSTRACTFive white corn hybrids were processed (nixtamalized) using 10 different processing conditions; tortillas were prepared to establish relationships between corn composition, physical characteristics, and nixtamalization process or product properties. Corn hybrids were characterized by proximate analysis and by measuring Stenvert hardness, Wisconsin breakage, percent floaters, TADD overs, thousand‐kernel weight, and test weight. Corn characteristics were correlated with process and product variables (effluent dry matter loss and pH; nixtamal moisture and color; masa moisture, color, and texture; and tortilla moisture, color, and rollability). Process and product variables such as corn solid loss, nixtamal moisture, masa texture, and tortilla color were influenced not only by processing parameters (cook temperature, cook time, and steep time) but also depended on corn characteristics. Significant regression equations were developed for nixtamalization dry matter loss (P < 0.05, r2 = 0.79), nixtamal moisture (P < 0.05, r2 = 0.78), masa gumminess (P < 0.05, r2 = 0.78), tortilla texture (P < 0.05, r2 = 0.77), tortilla moisture (P < 0.05, r2 = 0.80), tortilla calcium (P < 0.05, r2 = 0.93), and tortilla color a value (P < 0.05, r2 = 0.87).
Functional and Digestive Characteristics of Extruded Rice Flour
Authors: Bryant Rolfe J. , Kadan Ranjit S. , Champagne Elaine T. , Vinyard Bryan T. , Boykin Debbie 
Published: June 2007

ABSTRACTWaxy (short grain), long grain, and parboiled (long grain) rice flours were extruded using three different temperatures and five different water feed rates. The water absorption and water solubility index of the extrudates was 0.67–5.86 and 86.45–10.03%, respectively. The fat absorption index was similar to that of unextruded flours with an average value of 0.96 g/g ± 0.12. Bulk density decreased with an increase in moisture, except waxy rice, which had a quadratic relationship. The viscosity profiles for long grain and parboiled rice were similar. Both initially increased in viscosity (>130 RVU), then decreased to ≈40 RVU. The final viscosity was ≈60 RVU. Waxy rice viscosity remained low (<20 RVU), then doubled upon cooling. The main difference in the digestion profiles was due to temperature. The flours extruded at 100°C digested significantly slower than those extruded at 125 and 150°C. Significant differences were not detected for a given temperature and moisture (P > 0.05) except for long grain and parboiled rice extruded at 100°C and 15% added moisture (F = 4.48, P = 0.03) and 150°C and 20% added moisture (F = 3.72, P = 0.05). Moisture appeared to have little effect for a given temperature, except when parboiled rice was extruded at 150°C. The digestion rate for 11 and 25% added moisture was significantly less than that for 20% (P ≤ 0.05).
Effect of Sucrose on Glass Transition, Gelatinization, and Retrogradation of Wheat Starch
Authors: Jang J. K. , Lee S. H. , Cho S. C. , Pyun Y. R. 
Published: June 2007

ABSTRACTDifferential scanning calorimetry (DSC) was used to study the effect of sucrose on wheat starch glass transition, gelatinization, and retrogradation. As the ratio of sucrose to starch increased from 0.25:1 to 1:1, the glass transition temperature (Tg, Tg′) and ice melting enthalpy (ΔHice) of wheat starch‐sucrose mixtures (with total moistures of 40–60%) were decreased to a range of −7 to −20°C and increased to a range of 29.4 to 413.4 J/g of starch, respectively, in comparison with wheat starch with no sucrose. The Tg′ of the wheat starch‐sucrose mixtures was sensitive to the amount of added sucrose, and detection was possible only under conditions of excess total moisture of >40%. The peak temperature (Tm) and enthalpy value (ΔHG) for gelatinization of starch‐sucrose systems within the total moisture range of 40–60% were increased with increasing sucrose and were greater at lower total moisture levels. The Tg′ of the starch‐sucrose system increased during storage. In particular, the significant shift in Tg′ ranged between 15 and 18°C for a 1:1 starch‐sucrose system (total moisture 50%) after one week of storage at various temperatures (4, 32, and 40°C). At 40% total moisture, samples with sucrose stored at 4, 32, and 40°C for four weeks had higher retrogradation enthalpy (ΔH) values than a sample with no sucrose. At 50 and 60% total moisture, there were small increases in ΔH values at storage temperature of 4°C, whereas recrystallization of samples with sucrose stored at 32 and 40°C decreased. The peak temperature (Tp), peak width (δT), and enthalpy (ΔH) for the retrogradation endotherm of wheat starch‐sucrose systems (1:0.25, 1:0.5, and 1:1) at the same total moisture and storage temperature showed notable differences with the ratio of added sucrose. In addition, Tp increased at the higher storage temperature, while δT increased at the lower storage temperature. This suggests that the recrystallization of the wheat starch‐sucrose system at various storage temperatures can be interpreted in terms of δT and Tp.
Predicting Baking Performance from Rheological and Adhesive Properties of Rye Meal Suspensions During Heating
Authors: Repeckiene A. , Eliasson A.‐C. , Juodeikiene G. , Gunnarsson E. 
Published: June 2007

ABSTRACTRheological methods were used to study the behavior of rye meal suspensions during a time‐temperature treatment corresponding to the initial baking conditions (<70°C). Eight different rye cultivars were investigated, with four of the cultivars grown during two different years. Baking experiments included pan bread and hearth bread. Viscosity, falling number, and the amount of adhesive material present during heating were measured. The storage (G′) and loss (G″) moduli increased during a temperature sweep from 45°C, reaching a maximum at 62.1–67.1°C. At the same time, the amount of adhesive material increased. A further increase in temperature caused a decrease in G′ and G″, whereas the amount of adhesive material continued to increase. The mechanical spectra (G′ or G″ vs. frequency) showed that the rye meal suspensions had gel‐like behavior at 45°C which turned into behavior typical of a strong gel at 70°C. The rye meals performing the best in hearth bread baking gave intermediate values of G′ and G″ and high values of the phase shift (δ) at 45°C. During the temperature sweep, the G′ values of these rye meal suspensions increased slowly to a maximum of 62.1–67.1°C.
Comparison of Physicochemical Properties and Structures of Sugary‐2 Cornstarch with Normal and Waxy Cultivars
Authors: Perera C. , Lu Z. , Sell J. , Jane J. 
Published: June 2007

ABSTRACTStarches from normal, waxy, and sugary‐2 (su2) corn kernels were isolated, and their structures and properties determined. The total lipid contents of normal, waxy, and su2 corn starches were 0.84, 0.00, and 1.61%, respectively. Scanning electron micrographs showed that normal and waxy corn starch granules were spherical or angular in shape with smooth surfaces. The su2 starch granules consisted of lobes that resembled starch mutants deficient in soluble starch synthases. Normal and waxy corn starches displayed A‐type X‐ray patterns. The su2 starch showed a weak A‐type pattern. The chain‐length distributions of normal, waxy, and su2 debranched amylopectins showed the first peak chain length at DP (degree of polymerization) 13, 14, and 13, respectively; second peak chain length at DP 45, 49, and 49, respectively; and highest detectable DP of 80, 72, and 76, respectively. The su2 amylopectin showed a higher percentage of chains with DP 6–12 (22.2%) than normal (15.0%) and waxy (14.6%) amylopectins. The absolute amylose content of normal, waxy, and su2 starches was 18.8, 0.0, and 27.3%, respectively. Gel‐permeation profiles of su2 corn starch displayed a considerable amount of intermediate components. The su2 corn starch displayed lower gelatinization temperature, enthalpy change, and viscosity; a significantly higher enthalpy change for melting of amylose‐lipid complex; and lower melting temperature and enthalpy change for retrograded starch than did normal and waxy corn starches. The initial rate of hydrolysis (3 hr) of the corn starches followed the order su2 > waxy > normal corn. Waxy and su2 starches were hydrolyzed to the same extent, which was higher than normal starch after a 72‐hr hydrolysis period.
Mechanical Starch Damage Effects on Wheat Flour Tortilla Texture
Authors: Mao Yufeng , Flores Rolando A. 
Published: June 2007

ABSTRACTTo investigate the effects of mechanically damaged starch and flour particle size on the texture of fresh and stored flour tortillas, two commercial hard red winter wheat flour samples were reground four times using decreasing roll gaps. Tortillas were made with a modified hot‐press procedure. Texture characteristics were measured after tortillas were stored 2 hr (fresh tortilla), 2 days, and 4 days. Damaged starch and particle size significantly affected (P < 0.05) flour water absorption, dough extensibility and resistance, and dough viscosity. As damaged starch increased and particle size decreased, the flour tortillas became less stretchable, the maximum force of Kramer shear decreased, and firmness and rollability increased. The effects of damaged starch and particle size on stretchability and Kramer shear were greater in fresh tortillas than in stored tortillas and became smaller as the storage time increased. However, the effects of damaged starch and particle size on rollability and firmness were smaller in fresh tortillas than in stored tortillas but became greater as the storage time increased.