bluuebunny/crossref_metadata_2025_split huggingface dataset:
Canine serum keratan sulfate and hyaluronate concentrations. Relationship to age and osteoarthritis
Authors: Leipold Harry R. , Goldberg Ronald L. , Lust George 
Published: February 2007

AbstractElevated serum levels of keratan sulfate (KS) and hyaluronate (HA) in patients with osteoarthritis (OA) have been reported. We measured KS and HA in dogs to determine if there was an elevation of these serum glycosaminoglycans in a canine model of OA. A single intraarticular injection of 1 mg of chymopapain into a shoulder joint increased serum KS by tenfold, and HA by less than twofold, in 24 hours. Serum KS and HA levels were 3–5‐fold higher in dogs younger than 2 months of age than in older dogs. Serum KS and HA concentrations and synovial fluid KS concentrations were unrelated to spontaneous cartilage degeneration in 1‐year‐old dogs. Higher KS levels in synovial fluid correlated with higher KS levels in serum (r = 0.54, P < 0.025). The mean KS concentration in sera of older dogs (>3 years old) with OA was 37% higher than that in disease‐free controls, but the difference between the groups was not statistically significant. Thus, elevated levels of serum KS and HA do not appear to have clinical significance in this model of OA.
Subacute cutaneous lupus erythematosus during puva therapy for psoriasis: Case report and review of the literature
Authors: Dowdy Marcia J. , Nigra Thomas P. , Barth Werner F. 
Published: February 2007

AbstractLesions typical of subacute cutaneous lupus erythematosus developed in an elderly woman after 6 months of PUVA (8‐methoxypsoralen and longwave ultraviolet light) therapy for psoriasis. Pancytopenia, antibodies to double‐stranded DNA, and hypocomplementemia developed concurrently with the appearance of the cutaneous lesions. With discontinuation of photochemotherapy, the cutaneous lesions disappeared and the pancytopenia improved.
Microheterogeneity of alpha1‐acid glycoprotein in the detection of intercurrent infection in patients with rheumatoid arthritis
Authors: Pawlowski Tomasz , Mackiewicz Stefan H. , Mackiewicz Andrzej 
Published: February 2007

AbstractWe evaluated the clinical usefulness of determinations of α1 ‐acid glycoprotein (AGP) microheterogeneity in distinguishing patients who have active rheumatoid arthritis (RA) from those who have RA and an intercurrent infection. AGP microheterogeneity was studied by affinity electrophoresis with concanavalin A as the ligand, and the results were expressed as reactivity coefficients (RC). Significant differences were found between the mean RC ( ±SD) in healthy individuals (1.27 ± 0.16) and the mean RC in RA patients with intercurrent infection (1.74 ± 0.90), as well as with the mean RC in RA patients with grades III and IV disease activity (0.92 ± 0.18 and 0.81 ± 0.25, respectively). Moreover, an additional microheterogeneous form of AGP, similar to that observed in non‐RA patients with infections, was noted in RA patients with infections (sensitivity 100%, specificity 100%). The results show that an increase in AGP reactivity with concanavalin A is a sensitive indicator of intercurrent infection in patients with RA.
Genetic studies of Ro (SS‐A) and La (SS‐B) autoantibodies in families with systemic lupus erythematosus and primary sjögren's syndrome
Authors: Arnett Frank C. , Hamilton Robert G. , Reveille John D. , Bias Wilma B. , Harley John B. , Reichlin Morris 
Published: February 2007

AbstractUsing enzyme‐linked immunosorbent assays, autoantibodies to Ro (SS‐A) were detected in the sera of 21% of the first‐degree relatives and 11% of the second‐degree relatives of anti‐Ro–positive probands with systemic lupus erythematosus (SLE) or primary Sjögren's syndrome, as compared with 3% of normal control subjects (P = 0.003 and P = 0.09, respectively). In a parallel study, anti‐Ro occurred in 28% of the first‐degree relatives of unselected members of families of SLE patients, regardless of the proband's serologic status, compared with 6% of the relatives from normal healthy families. Antibodies to La and to Sm/nuclear RNP were infrequent. Anti‐Ro occurred in 41% of the relatives considered to have a dominant, non–HLA‐linked “autoimmune trait” by virtue of having any autoimmune disorder and/or serologic abnormality (antinuclear antibodies, anti–single‐stranded DNA, or biologic false‐positive VDRL test result), as compared with only 2% of the healthy, seronegative relatives without the trait (P = 0.009). Moreover, HLA–DR2 and/or DR3 occurred in 90% of anti‐Ro–positive subjects, regardless of their clinical status. These results demonstrate that the Ro autoantibody response occurs frequently in relatives of patients with SLE and is genetically mediated by both major histocompatibility complex and non–major histocompatibility complex effects.
Increased proto‐oncogene expression in peripheral blood T lymphocytes from patients with systemic sclerosis
Authors: Kahan Alice , Gerfaux Jacqueline , Kahan André , Joret Anne‐Marie , Menkès Charles J. , Amor Bernard 
Published: February 2007

AbstractThe expression of c‐myc, c‐myb, and c‐ras protooncogenes, determined using RNA hybridization techniques (slot‐blot), was significantly increased in peripheral blood T lymphocytes, but not in B cells, from 17 patients with systemic sclerosis with diffuse scleroderma, compared with the expression in normal control subjects. The magnitude of expression of c‐myc and c‐myb tended to be higher in patients with early, active disease. These results demonstrate an in vivo activation of T cells from systemic sclerosis patients, which may play an important role in the pathogenesis of the disease.
Aerobic fitness in patients with fibrositis. A controlled study of respiratory gas exchange and 133xenon clearance from exercising muscle
Authors: Bennett Robert M. , Clark Sharon R. , Goldberg Linn , Nelson Donna , Bonafede R. Peter , Porter John , Specht David 
Published: February 2007

AbstractAerobic fitness was evaluated in 25 women with fibrositis, by having them exercise to volitional exhaustion on an electronically braked cycle ergometer. Compared with published standards, >80% of the fibrositis patients were not physically fit, as assessed by maximal oxygen uptake. Compared with matched sedentary controls, fibrositis patients accurately perceived their level of exertion in relation to oxygen consumption and attained a similar level of lactic acidosis, as assessed by their respiratory quotient and ventilatory threshold. Exercising muscle blood flow was estimated by 133xenon clearance in a subgroup of 16 fibrositis patients and compared with that in 16 matched sedentary controls; the fibrositis patients exhibited reduced 133xenon clearance. These results indicate a need to include aerobic fitness as a matched variable in future controlled studies of fibrositis and suggest that the “detraining phenomenon” may be of relevance to the etiopathogenesis of the disease.
Nuclear imaging and clinical features of childhood reflex neurovascular dystrophy: Comparison with adults
Authors: Goldsmith Donald P. , Vivino Frederick B. , Eichenfield Andrew H. , Athreya Balu H. , Heyman Sydney 
Published: February 2007

AbstractReflex neurovascular dystrophy (RND) is less common in children than in adults, and differences in onset, clinical course, response to treatment, and degree of disability suggest a different pathogenesis. We have assessed the usefulness of nuclear imaging in 15 children with RND who were evaluated from March 1983 to September 1985. Abnormal findings on 3‐phase bone scans were observed in 14 children, with diffusely decreased bone uptake at the symptomatic site being the most common observation. This contrasts sharply with previous reports of diffusely increased uptake in most adults with RND.
Effects of salicylate on chondrocytes from osteoarthritic and contralateral knees of dogs with unilateral anterior cruciate ligament transection
Authors: Slowman‐Kovacs Sally D. , Albrecht Marjorie E. , Brandt Kenneth D. 
Published: February 2007

AbstractSalicylates suppress net glycosaminoglycan synthesis in articular cartilage. The inhibitory effect is greater in osteoarthritic (OA) cartilage than in normal cartilage. Whether the isolated OA chondrocyte is inherently more susceptible to the effects of salicylate on glycosaminoglycan metabolism has not been determined. The results of this study show that, after isolation from the extracellular matrix, normal and OA chondrocytes in suspension culture are similarly susceptible to the metabolic effects of salicylate. However, chondrocytes from the contralateral knees of dogs with unilateral OA were notably resistant to the effects of salicylate.
Hepatic infarction in a patient with the lupus anticoagulant
Authors: Mor F. , Beigel Y. , Inbal A. , Goren M. , Wysenbeek A. J. 
Published: February 2007

AbstractWe describe a 31‐year‐old patient with missed abortion, thrombocytopenia, and clinical, laboratory, and radiologic evidence of hepatic infarction. On evaluation, she was found to have the lupus anticoagulant. The association between enhanced thrombosis and the lupus anticoagulant is discussed, and previously reported thrombotic complications are described. The etiology, clinical course, and radiologic features of liver infarction are summarized, and the importance of recognizing and treating this form of hypercoagulability is stressed. To our knowledge, this is the first description of liver infarction associated with the lupus anticoagulant.
A simplified twenty‐eight–joint quantitative articular index in rheumatoid arthritis
Authors: Fuchs Howard A. , Brooks Raye H. , Callahan Leigh F. , Pincus Theodore 
Published: February 2007

AbstractWe describe a joint index that includes only 28 joints: 10 proximal interphalangeal joints of the fingers, 10 metacarpophalangeal joints, and the wrists, elbows, shoulders, and knees. These joints are evaluated for swelling, tenderness, and limited motion, with the findings scored as abnormal or normal. The 28‐joint index is considerably easier to use than traditional methods, and appears to yield as much information in terms of correlation with other measures of clinical status, including hand radiograph scores, American Rheumatism Association functional class, grip strength, walking time, and activities of daily living questionnaire scores (r = 0.25–0.53, P < 0.001). Simplified joint counts might facilitate acquisition of quantitative articular data in research and clinical rheumatologic settings.
Decreased expression of interleukin‐2 binding molecules (p70/75) in T cells from patients with systemic lupus erythematosus
Authors: Tanaka Toshio , Saiki Osamu , Negoro Shigeru , Igarashi Tsuyoshi , Kuritani Taro , Hara Hideki , Suemura Masaki , Kishimoto Susumu 
Published: February 2007

AbstractUsing radiolabeled interleukin‐2 (IL‐2), affinity cross‐linking and binding assays revealed that the expression of intermediate‐affinity IL‐2 binding molecules (p70/75) on freshly prepared T cells from patients with systemic lupus erythematosus (SLE) was significantly decreased in comparison with that in normal subjects. The proliferative response of T cells to high doses of IL‐2 was also reduced in patients with SLE. The decreased expression of p70/75 reflects the hyporespon‐siveness to IL‐2 of T cells in patients with SLE.
Treatment of the anemia of rheumatoid arthritis with recombinant human erythropoietin: Clinical and in vitro studies
Authors: Means Robert T. , Olsen Nancy J. , Krantz Sanford B. , Dessypris Emmanuel N. , Graber Stanley E. , Stone William J. , O'Neil Vicki L. , Pincus Theodore 
Published: February 2007

AbstractTwo anemic patients with rheumatoid arthritis were treated with recombinant human erythropoietin (EPO) for 5 months. Both patients showed significant increases in hematocrit, red cell volumes, and marrow erythroid and megakaryocyte progenitor cells. No significant toxic effects from EPO were observed. These data indicate that EPO may be effective in overcoming the pathogenetic factors that limit erythropoiesis in rheumatoid arthritis.
Pregnancy does not cause systemic lupus erythematosus to worsen
Authors: Lockshin Michael D. 
Published: February 2007

AbstractTo evaluate risk for exacerbation of systemic lupus erythematosus (SLE) during pregnancy, we pro‐spectively evaluated 80 pregnant women with SLE for manifestations of disease activity. Fifty‐three of these women were not taking prednisone at the time of conception. Disease activity was scored in 4 ways: global assessment, prednisone therapy, cumulative number of organ systems with abnormalities, and display of abnormalities of each organ system. No patient received prophylactic therapy to prevent disease exacerbation. Thrombocytopenia, proteinuria, and hypocomplementemia were the most common abnormalities and were usually attributable to the pregnancy complications of preeclampsia and anticardiolipin antibody syndrome rather than to SLE. If all possible abnormalities were attributed to SLE, disease exacerbation occurred in <25% of all patients; if only SLE‐specific abnormalities were counted, disease exacerbation occurred in <13%. Worsening of SLE is uncommon in pregnancy, and prophylactic prednisone therapy is unnecessary.
Methotrexate in rheumatoid arthritis. Toxic effects as the major factor in limiting long‐term treatment
Authors: Alarcóan Graciela S. , Tracy Irene C. , Blackburn Warren D. 
Published: February 2007

AbstractIn an effort to understand the prognostic features that may influence the probability of a patient's continuing to take methotrexate (MTX) over time, we studied 152 rheumatoid arthritis patients treated with MTX between 1981 and 1986. The overall probability of continuing to take MTX was 71.2% at 1 year, 55.5% at 3 years, 50% at 5 years, and 49% at 6 years. By univariate analysis, patients who started MTX therapy later in the study, American blacks, younger patients, those with less severe disease, and those with less frequent or less severe toxic events appeared to have a better probability of continuing the drug therapy. When these parameters were evaluated by multivariate analysis, only the time when MTX was started and the occurrence of toxic effects independently influenced the probability of continuing MTX. Thus, by current practice standards, toxic effects emerge as the main reason for MTX discontinuation.
Cross‐reactive antiidiotypic antibodies against human rheumatoid factors from patients with juvenile rheumatoid arthritis
Authors: Moore Terry L. , Osborn Thomas G. , Dorner Robert W. 
Published: February 2007

AbstractWe prepared antiidiotypic (anti‐Id) antibody to 2 polyclonal IgM rheumatoid factors (IgM‐RF) and 2 polyclonal “hidden” IgM‐RF. The anti‐Id antibodies were isolated by chromatography on Sepharose 4B, to which was bound rabbit anti‐human IgG Fc fragments. F(ab′)2 fragments from the anti‐Id antibodies were generated by pepsin digestion and isolated by gel filtration. The anti‐Id antibodies directed against RF from 4 patients with juvenile rheumatoid arthritis (JRA) were tested by an inhibition hemolytic assay for crossreactivity with IgM‐RF from 4 adult patients with rheumatoid arthritis, 6 patients with JRA, and 13 JRA patients with hidden RF. The 4 anti‐Id antibodies had variable cross‐reactivity with the isolated adult RA RF, JRA RF, and JRA hidden RF. Similar results were obtained by a direct‐binding enzyme‐linked immunosorbent assay for the anti‐Id antibodies. The broad pattern of cross‐reactivity was apparently unrelated to a particular amino acid sequence, but was associated with the antigen‐binding site of IgM‐RF. These results suggest the possibility that the anti‐Id antibodies prepared against isolated RF obtained from JRA patients bear the “internal image” of antigen; that is, the Fc region of human IgG. These anti‐Id antibodies may be generated in JRA patients and may possess specific immunomodulatory properties.
Plasma dna in systemic lupus erythematosus. Characterization of cloned base sequences
Authors: Li Jin Zhau , Steinman Charles R. 
Published: February 2007

AbstractLittle is known about plasma DNA in patients with systemic lupus erythematosus (SLE). Previous studies have suggested that it may, in fact, not be derived from the random nucleolysis that might be expected to accompany cell death, which might have been the simplest explanation of its origin. To extend the results of other studies, plasma DNA, obtained from a subgroup of SLE patients who had large amounts of immunoprecipitable plasma DNA, was cloned into a plasmid vector, and the nucleotide base sequences were studied by nucleic acid hybridization. One possible explanation for the anomalous renaturation kinetics of plasma DNA in SLE—namely, that it contained non‐human genomic base sequences—was rendered untenable by the fact that 51 consecutive DNA clones from 2 SLE patients were each shown to contain human genomic DNA sequences. Plasma DNA from SLE patients also differed from human genomic control DNA in that those sequences derived from the highly repetitive fraction of human DNA were specifically enriched in one such sequence, Alu, which constituted 55% of the repeat sequences in the plasma DNA clones as compared with 13% in control DNA clones. There was a decrease in the frequency of non‐Alu repeat sequences (9% for plasma DNA compared with 23% in control DNA). These differences were statistically significant, and they indicate that SLE plasma DNA contains a nonrandom selection of human genomic base sequences. Several explanations for these findings are considered.
The effect of tumor necrosis factor α and γ‐interferon on the resorption of human articular cartilage and on the production of prostaglandin e and of caseinase activity by human articular chondrocytes
Authors: Bunning Rowena A. D. , Russell R. Graham G. 
Published: February 2007

AbstractIn cultured human articular chondrocytes, addition of tumor necrosis factor α (TNFα1) stimulated caseinase activity over the range of 10−11M to 10−7M and stimulated prostaglandin E (PGE) production over the range of 10−10M to 10−7M. Maximal stimulation was observed at 10−8M TNFα for both activities. Gamma‐interferon (γ‐IFN) had a variable effect on PGE production and no significant effect on caseinase activity in articular chondrocyte cultures over a concentration range of 0.1–1,000 units/ml. Co‐incubation of TNFα with γ‐IFN enhanced PGE production and decreased caseinase activity. Concentrations as low as 1 unit/ml of γ‐IFN had significant effects on TNF‐stimulated production of PGE and on caseinase activity. Resorption of human articular cartilage was stimulated by TNFα (10−7M) and was inhibited by γ‐IFN (1,000 units/ml). It is possible that cartilage breakdown in vivo may be modulated by such interactions between cytokines.
Rheumatic syndromes and β2‐microglobulin amyloidosis in patients receiving long‐term peritoneal dialysis
Authors: Cornéalis F. , Bardin T. , Faller B. , Verger C. , Allouache M. , Raymond P. , Rottembourg J. , Tourlière D. , Benhamou C. , Noel L. H. , Kuntz D. 
Published: February 2007

AbstractWe studied 56 patients who had been receiving peritoneal dialysis for >3 years, to investigate the prevalence of rheumatic diseases suggestive of β2‐microglobulin (β2m) amyloid deposition. Eight patients were found to have carpal tunnel syndrome, 16 had chronic shoulder pain, 8 had subchondral bone cysts, and 13 had destructive arthropathies. Amyloid reacting with anti‐β2 m was demonstrated in the hip synovium of 1 patient. Serum β2m levels were elevated in all patients. These data suggest that peritoneal dialysis, like hemodialysis, may lead to the development of an arthropathy associated with β2m accumulation and β2m amyloid deposition.
Double‐blind trial of recombinant γ‐interferon versus placebo in the treatment of rheumatoid arthritis
Authors: Cannon Grant W. , Pincus Seth H. , Emkey Ronald D. , Denes Alex , Cohen Selwyn A. , Wolfe Frederick , Anthony Saway P. , Jaffer Adrian M. , Weaver Arthur L. , Cogen Lewis , Schindler John D. 
Published: February 2007

AbstractOne hundred five patients were enrolled in a 12‐week, randomized, prospective, double‐blind, placebo‐controlled trial of recombinant human γ‐interferon (rHuγ‐IFN) for the treatment of rheumatoid arthritis. Fifty‐four patients received rHuγ‐IFN and 51 received placebo. Forty‐two patients in each group completed the 12‐week trial. Some clinical improvement occurred in both groups of patients. Although the improvement with rHuγ‐IFN was greater than that with placebo, the differences were generally not statistically significant.
Prevalence of scleroderma spectrum disorders in the general population of south carolina
Authors: Maricq Hildegard R. , Weinrich Martin C. , Keil Julian E. , Smith Edwin A. , Harper Frank E. , Nussbaum Alan I. , Carwile Leroy E. , McGregor Andrew R. , Diat Francoise , Rosal Erick J. 
Published: February 2007

AbstractThe prevalence of scleroderma spectrum disorders (including systemic sclerosis [SSc] meeting the American Rheumatism Association criteria and the less typical disorders meeting only our study criteria) was determined in a random sample of 6,998 subjects from the general population of South Carolina. The results suggest that the prevalence of these disorders may range from 67 to 265 per 100,000, which is 4.9 to 19.2 times higher than previously reported for definite SSc. The ratio of nondefinite cases to definite cases of SSc (those meeting American Rheumatism Association criteria) was 2.5. Most of the nondefinite cases were unrecognized prior to our study, which suggests the need for improved early diagnosis of scleroderma spectrum disorders. Brief histories of the 7 patients with scleroderma spectrum disorders whose cases formed the basis for our calculation of prevalence rates are included in this report.
Pristane‐induced arthritis. the immunologic and genetic features of an experimental murine model of autoimmune disease
Authors: Wooley Paul H. , Seibold James R. , Whalen Janey D. , Chapdelaine Joan M. 
Published: February 2007

AbstractPristane was injected intraperitoneally into mice of several strains, inducing an inflammatory seropositive arthritis in susceptible strains. The evolving histologic features included synovial hyperplasia, periostitis, and progressive marginal erosions. Multiple serologic immune abnormalities, including rheumatoid factor and anticollagen antibodies, also developed. Genetic analysis indicated that the major histocompatibility complex (H‐2), C5 hemolytic complement (Hc), Newcastle disease virus‐induced interferon (IF‐1), and athymic (nu/nu) loci were involved in regulating susceptibility to pristane‐induced arthritis. This experimental murine disease may provide a novel model of rheumatoid arthritis.
Altered metabolism of poly(adp‐ribose) in the peripheral blood lymphocytes of patients with systemic lupus erythematosus
Authors: Sibley John T. , Haug Brenda L. , Lee Jeremy S. 
Published: February 2007

AbstractA method was developed to measure poly(ADP‐ribose) metabolism in peripheral blood lymphocytes. The technique involved the isolation of lymphocytes on Ficoll gradients, followed by lysis with 5M NaCl. The synthesis and degradation of poly(ADP‐ribose) in this crude lysate, measured by the incorporation of 3H‐labeled NAD into acid‐precipitable counts, was compared in 18 patients with systemic lupus erythematosus (SLE), 10 patients with rheumatoid arthritis, and in 10 control patients without rheumatoid arthritis. Patients with SLE showed a 70% decrease in poly(ADP‐ribose) synthesis (P < 0.001); this decreased synthesis persisted even with the addition of histones or DNase. We present possible explanations of the role of poly(ADP‐ribose) in SLE.