bluuebunny/crossref_metadata_2025_split huggingface dataset:
FUT7 antisense sequence inhibits the expression of FUT7/sLeX and adhesion between embryonic and uterine cells
Authors: Liu Shuai , Zhang Yuanyuan , Liu Yuejian , Qin Huamin , Wang Xiaoqi , Yan Qiu 
Published: June 2008

AbstractImplantation is a complex developmental event that is initiated by recognition and adhesion of the embryo to the endometrial epithelium. sLeX is an oligosaccharide antigen acting as the ligand of L‐selectin, and is stage‐specifically expressed in the endometrial epithelium. The adhesion system mediated by L‐selectin and sLeX oligosaccharide plays an important role in this process. FUT7 is a key enzyme for sLeX synthesis, and the regulation of sLeX through FUT7 may influence maternal–fetal recognition. In this study, we observed the effect of FUT7 antisense oligodeoxynucleotide on the expression of FUT7 and sLeX, as well as adhesion in an in vitro implantation model consisting of the human uterine epithelial cell line RL95‐2 and the human embryonic cell line JAR. Results showed that the expression of FUT7 was significantly decreased, compared with controls, after FUT7 antisense oligodeoxynucleotide transfection into RL95‐2 cells, as determined by RT‐PCR, Western blotting, and indirect immunofluorescence. Synthesis of sLeX was also decreased, consistent with the FUT7 decrease, as shown by indirect immunofluorescence. The adhesion of embryonic cells to uterine epithelial cells was significantly reduced (P < 0.01) compared with the control. These data indicate that the use of a FUT7 antisense oligodeoxynucleotide can cause a significant reduction of both FUT7 and sLeX antigen, and thereby inhibit the adhesion of embryo cells to endometrium. This approach may provide a new way to regulate reproduction. © 2008 IUBMB IUBMB Life, 60(7): 461–466, 2008
Silencing survivin gene expression promotes apoptosis of human breast cancer cells through a caspase‐independent pathway
Authors: Croci Diego O. , Cogno Ingrid S. , Vittar Natalia B. Rumie , Salvatierra Edgardo , Trajtenberg Felipe , Podhajcer Osvaldo L. , Osinaga Eduardo , Rabinovich Gabriel A. , Rivarola Viviana A. 
Published: June 2008

AbstractSurvivin is recognized as an attractive target in cancer therapy because of its selective overexpression in the majority of tumors. Upregulated expression of this protein correlates with increased tumor grade, recurrence risk and decreased cancer patients survival. In this study, we assessed the efficacy of two survivin‐specific small interfering RNA (siRNA) constructs to inhibit T47D human breast cancer cell growth. After siRNA transfection, T47D cells showed a significant reduction in proliferation and survival exhibiting clear signs of apoptosis. pSil_1 that targeted exon 1 exhibited a stronger inhibitory effect on cell growth, and increased cell apoptosis compared to pSil_30 that targeted exon 4. Cell apoptosis was found to be mediated by translocation of the mitochondrial apoptosis inducing factor (AIF), while no changes were observed in caspase‐3 activation and Bid cleavage. Thus, silencing survivin expression using siRNA strategies represents a suitable therapeutic approach to selectively modulate the survival and growth of human breast cancer cells. J. Cell. Biochem. 105: 381–390, 2008. © 2008 Wiley‐Liss, Inc.
Issues and opportunities in accelerator mass spectrometry for stable isotopes
Authors: Matteson Sam 
Published: June 2008

AbstractAccelerator mass spectrometry (AMS) has developed in the last 30 years many notable applications to the spectrometry of radioisotopes, particularly in radiocarbon dating. The instrumentation science of trace element AMS (TEAMS) that analyzes stable isotopes, also called Accelerator SIMS or MegaSIMS, while unique in many features, has also shared in many of these significant advances and has pushed TEAMS sensitivity to concentration levels surpassing many competing mass spectroscopic technologies. This review examines recent instrumentation developments, the capabilities of the new instrumentation and discernable trends for future development. © 2008 Wiley Periodicals, Inc., Mass Spec Rev 27: 470–484, 2008
Analysis of environmental stress response on the proteome level
Authors: Nesatyy Victor J. , Suter Marc J.‐F. 
Published: June 2008

AbstractThousands of man‐made chemicals are annually released into the environment by agriculture, transport, industries, and other human activities. In general, chemical analysis of environmental samples used to assess the pollution status of a specific ecosystem is complicated by the complexity of the mixture, and in some cases by the very low toxicity thresholds of chemicals present. In that sense, a proteomics approach, capable of detecting subtle changes in the level and structure of individual proteins within the whole proteome in response to the altered surroundings, has obvious applications in the field of ecotoxicology. In addition to identifying new protein biomarkers, it can also help to provide an insight into underlying mechanisms of toxicity. Despite being a comparatively new field with a number of caveats, proteomics applications have spread from microorganisms and plants to invertebrates and vertebrates, gradually becoming an established technology used in environmental research. This review article highlights recent advances in the field of environmental proteomics, mainly focusing on experimental approaches with a potential to understand toxic modes of action and to identify novel ecotoxicological biomarkers. © 2008 Wiley Periodicals, Inc., Mass Spec Rev 27: 556–574, 2008
Component evolution in a secure wireless sensor network
Authors: Bloznelis M. , Jaworski J. , Rybarczyk K. 
Published: June 2008

AbstractWe study a connectivity property of a secure wireless network that uses random pre‐distribution of keys. A network is composed of n sensors. Each sensor is assigned a collection of d different keys drawn uniformly at random from a given set of m keys. Two sensors are joined by a communication link if they share a common key. We show that for large n with high probability the connected component of size Ω(n) emerges in the network when the probability of a link exceeds the threshold 1/n. Similar component evolution is shown for networks where sensors communicate if they share at least s common keys. © 2008 Wiley Periodicals, Inc. NETWORKS, 2009
On the L(h, k)‐labeling of co‐comparability graphs and circular‐arc graphs
Authors: Calamoneri Tiziana , Caminiti Saverio , Petreschi Rossella , Olariu Stephan 
Published: June 2008

AbstractGiven two nonnegative integers h and k, an L(h, k)‐labeling of a graph G = (V, E) is a map from V to a set of integer labels such that adjacent vertices receive labels at least h apart, while vertices at distance at most 2 receive labels at least k apart. The goal of the L(h, k)‐labeling problem is to produce a legal labeling that minimizes the largest label used. Since the decision version of the L(h, k)‐labeling problem is NP‐complete, it is important to investigate classes of graphs for which the problem can be solved efficiently. Along this line of thought, in this article we deal with co‐comparability graphs, its subclass of interval graphs, and circular‐arc graphs. To the best of our knowledge, ours is the first reported result concerning the L(h, k)‐labeling of co‐comparability and circular‐arc graphs. In particular, we provide the first algorithm to L(h, k)‐label co‐comparability, interval, and circular‐arc graphs with a bounded number of colors. Finally, in the special case where k = 1 and G is an interval graph, our algorithm improves on the best previously‐known ones using a number of colors that is at most twice the optimum. © 2008 Wiley Periodicals, Inc. NETWORKS, 2009
Epoxy resin/exfoliated clay hybrid materials with high thermal properties
Authors: Fu Tiezhu , Yu Lianxiang , Wang Zhe , Yu Wenzhi , Zhao Chengji , Zhong Shuangling , Cui Jiwen , Shao Ke , Na Hui 
Published: June 2008

AbstractA new type of organophilic clay grafted with the protonated 4, 4′‐methylenediamine was prepared. The epoxy was modified using the organophilic clay and was prepared by “acetone‐clay slurry” method. The morphology of the clay platelets into epoxy matrix were characterized by wide angle X‐ray diffraction and transmission electron microscopy. The results indicated that the epoxy resin/exfoliated clay hybrid materials were successfully prepared and the exfoliation of clay into epoxy matrix occurred in the curing process. The excellent thermal mechanical properties of the hybrid materials with the values of the storage modulus and glass transition temperature (Tg) of the hybrid materials were studied by dynamic mechanical analysis. When 7 wt% of modified clay was added into epoxy, the storage modulus of hybrid materials increased to 1131 MPa at 110°C, and 51 MPa at 220°C in comparison to the pristine cured epoxy (661 MPa at 110°C and 6 MPa at 220°C). The high thermal stabilities of the hybrid materials especially in high temperature were also been researched by thermogravimetric analysis. POLYM. COMPOS., 2009. © 2008 Society of Plastics Engineers
Modelling the nonlinear shear stress–strain behavior of a carbon fabric reinforced polyphenylene sulphide from rail shear and [(45°,−45°)]4s tensile test
Authors: De Baere Ives , Van Paepegem Wim , Degrieck Joris 
Published: June 2008

AbstractIn this article, the nonlinear shear stress–strain relationship of a carbon fabric‐reinforced polyphenylene sulphide is investigated by performing and comparing both the [+45°/−45°]ns tensile test and the three‐rail shear test. First, quasi‐static and hysteresis tests are performed to obtain the data necessary for the material model. Then, the material constants are optimized by comparing finite element simulations with the data derived from the experiments. The conducted experiments are simulated and the results are compared with the experiments, with excellent correspondence. POLYM. COMPOS., 2009. © 2008 Society of Plastics Engineers
Formation of Boron‐doped region using spin‐on dopant: investigation on the impact of metallic impurities
Authors: Jourdan J. , Veschetti Y. , Dubois S. , Desrues T. , Monna R. 
Published: June 2008

AbstractInvestigation on the electrical properties ofp+‐dopedregions formed by spin‐on‐dopant (SOD) technique was achieved. Using this technique, boron‐diffused regions were formed on bothp‐typeandn‐typefloat zone wafers. Homogeneous sheet resistances were obtained for both types of wafers. Bulk properties were investigated by measuring effective carrier lifetime. An iron contamination was observed after the boron diffusion step and interstitial iron concentrations were deduced from lifetime measurements. More investigations proved that the iron was initially present within the SOD film. A phosphorus diffusion allows to remove this bulk contamination, leading to an improvement of effective lifetime values. Nevertheless, the corresponding emitter saturation current density was estimated onn‐typewafers and presented a high value. It is likely that this poor electrical quality is the consequence of a high iron concentration which remains in the diffused region. Some possibilities are suggested to avoid or to limit this contamination. Copyright © 2008 John Wiley & Sons, Ltd.
Modification of gamma‐radiation response in mice by green tea polyphenols
Authors: Lee Hae June , Kim Joong Sun , Moon Changjong , Kim Jong Choon , Lee Yun Sil , Jang Jong Sik , Jo Sung Kee , Kim Sung Ho 
Published: June 2008

AbstractIn this study we evaluated the effect of water extracts of green tea (GT) and mixtures of green tea polyphenols (GTPs), epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and epicatechin (EC) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with gamma‐ray. The radioprotective effect of green tea was compared with the effect of diethyldithiocarbamate (DDC). Jejunal crypts were protected by pretreatment of GT and ECG. Administration of GT, GTPs and EC prior to irradiation resulted in an increase in the formation of endogenous spleen colonies. The frequency of apoptosis in crypt cells was also reduced by pretreatment of GT, GTPs, EGCG, ECG and EGC. In the experiment on the effect of catechins, the effects were partly contradicted in irradiated mice. The rank order of activity was ECG > EGC > EGCG > EC on intestinal crypt survival assay, EC > EGC > ECG > EGCG on the spleen colony formation assay, EGCG > EGC > EC > ECG on inhibiting the death of cells caused by apoptosis. The results indicate that GT and GTPs may have a major radioprotective effect. Each one of the catechins was a much less effective radioprotector, suggesting that total extract or a mixture of GTPs may be more effective than individual catechins. Copyright © 2008 John Wiley & Sons, Ltd.
Dynamics of photoexcitations with interchain coupling in conjugated polymers
Authors: de Oliveira Neto Pedro Henrique , da Cunha Wiliam Ferreira , Gargano Ricardo , e Silva Geraldo Magela 
Published: June 2008

AbstractIn this article, we study the photoexcitation dynamics of two coupled trans‐polyacetylene chains. The approach used was an extended version of the Su, Scherieffer, and Heeger model for a pair of interacting chains and impurity. An electron excitation from one fully dimerized chain to another was simulated. The chain length influence were analyzed and shown to be of importance when the spectrum is concerned. It was also observed that the electron excitation generates a pair of neutral solitons on the second chain. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Conformations and charge distributions of diazocyclopropanes
Authors: Borges Itamar 
Published: June 2008

AbstractThree diazo‐substituted cyclopropane compounds, which have been suggested as new potential high energy compounds, were studied employing the B3LYP‐DFT/6‐31G(d,p) method. Geometries were optimized. Distributed multipole analysis, computed from the B3LYP‐DFT/6‐31G(d,p) density matrix, was used to describe the details of the molecular charge distribution of the three molecules. It was verified that electron withdrawing from the C ring atoms and charge build‐up on the N atoms bonded to the ring increased with the number of diazo groups. These effects were related to increased sensitivity to impact and easiness of CN bond breaking in the three compounds. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Empirical analysis of the Lieb–Oxford bound in ions and molecules
Authors: Odashima Mariana M. , Capelle K. 
Published: June 2008

AbstractUniversal properties of the Coulomb interaction energy apply to all many‐electron systems. Bounds on the exchange‐correlation energy, in particular, are important for the construction of improved density functionals. Here we investigate one such universal property—the Lieb‐Oxford lower bound—for ionic and molecular systems. In recent work [J Chem Phys 127, 054106 (2007)], we observed that for atoms and electron liquids this bound may be substantially tightened. Calculations for a few ions and molecules suggested the same tendency, but were not conclusive due to the small number of systems considered. Here we extend that analysis to many different families of ions and molecules, and find that for these, too, the bound can be empirically tightened by a similar margin as for atoms and electron liquids. Tightening the Lieb‐Oxford bound will have consequences for the performance of various approximate exchange‐correlation functionals. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Computational study of the solvation of protoporphyrin IX and its Fe2+ complex
Authors: Guizado Teobaldo Cuya , da Rocha Pita Samuel , Wanderley Louro Sonia R. , Geraldo Pascutti Pedro 
Published: June 2008

AbstractMolecular dynamics (MD) simulations of a well known hydrophobic structure, the heme (ferroprotoporphyrin IX) and its precursor in the heme synthesis, protoporphyrin IX (PPIX) are presented. The objective of the present study is to determine the stability of both structures in an aqueous medium, as well as the structure‐solvent relation, hydration shells, and discuss their implications for biological processes. The density functional theory (DFT) is used for the electronic and structural characterization of both PPIX and its Fe2+ complex. A classical approach based on the Gromacs package is used for the MD. The radial distribution function g(r) is used to examine the allocation of water molecules around different regions of the porphyrins. The calculations demonstrate the heterogeneous character of the porphyrins with respect to the affinity with water molecules, the general hydrophobic character of the porphyrin ring bonded or not to the ion Fe, the hydrophilic character of the carboxylic oxygen that is unchanged upon iron binding, and the low hydrophilicity of Fe2+ in the heme. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
DFT/PCM investigation of the Mn(II) chemical speciation in aqueous solution
Authors: de Abreu Heitor Avelino , Guimarães Luciana , Duarte Hélio Anderson 
Published: June 2008

AbstractChemical speciation of Mn(II) in aqueous solution has been investigated by means of density functional methods. Different geometries and electronic states of all species arising from the Mn(II) hydrolysis have been calculated. The Gibbs free energy of the hydrolysis reaction and the respective equilibrium constants have been estimated and compared with the available experimental data. Polarizable continuum model (PCM‐UAHF/6‐31+G(d)) was used to estimate the solvation energy. The ionization potential of the predominant species are linearly dependent on the number of hydroxyls in their structure. The Mn(II)/Mn(III) oxidation potential estimate is in good agreement with the experimental value, about 0.2 V smaller. The estimated hydrolysis reaction energies are in good agreement with the experimental values with an error of about 3 kcal mol−1 (PBE/TZVP). The unique exception is the [Mn(OH)3]− hydrolysis constant which is not in good agreement with the experimental value. This is attributed to inaccuracy of the presently available experimental value and the PCM solvation free energy estimate. The results shown in this work reinforce the importance of the chemical speciation in order to understand at a molecular level the mechanism of processes involving transition metal ions. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Relativistic corrections to the partial wave expansion of two‐electron atoms
Authors: Kutzelnigg Werner 
Published: June 2008

AbstractThe asymptotic behavior of the partial wave expansion (or the CI expansion in terms of AOs with angular quantum number l) of the leading relativistic correction for two‐electron atomic ions in a 1S state is studied analytically. In agreement with earlier numerical findings, the partial wave increments of the leading relativistic correction go as (l + 1/2)−2, whereas the nonrelativistic increments go as (l + 1/2)−4. The coefficient of the leading term is 2 α2 π2 ∫ | ψ(r,r,0)|2r3dr for the Dirac Coulomb operator, and surprisingly also the full Dirac‐Breit operator, while the Gaunt term gives a contribution of −4α2π2∫|ψ(r,r,0)|2r3dr. The results obtained here rely entirely on the nonrelativistic correlation cusp, and do not require assumptions on a no‐pair projection or a short‐range regularization of the electron interaction. They do not give any information on what happens beyond the leading order of relativistic corrections. © 2008 Wiley Periodicals, Inc. Int J Quantum Chem, 2008
Lead in soil by field‐portable x‐ray fluorescence spectrometry—an examination of paired In Situ and laboratory ICP‐AES results
Authors: Binstock David A. , Gutknecht William F. , McWilliams Andrea C. 
Published: June 2008

AbstractA major aspect of lead hazard control is the evaluation of soil lead hazards around housing coated with lead‐based paint. The use of field‐portable X‐ray fluorescence (FPXRF) to do detailed surveying, with limited laboratory confirmation, can provide lead measurements in soil (especially for planning abatement activities) in a far more cost‐efficient and timely manner than laboratory analysis. To date, one obstacle to the acceptance of FPXRF as an approved method of measuring lead in soil has been a lack of correspondence between field and laboratory results. In order to minimize the differences between field and laboratory results, RTI International (RTI) has developed a new protocol for field drying and sieving soil samples for field measurement by FPXRF. To evaluate this new protocol, composite samples were collected in the field following both U.S. Department of Housing and Urban Development (HUD) guidelines and ASTM International (ASTM) protocols, measured after drying by FPXRF, and returned to the laboratory for confirmatory inductively coupled plasma atomic emission spectroscopy (ICP‐AES) analysis. Evaluation of study data from several diverse sites revealed no statistical difference between paired FPXRF and ICP‐AES measurements using the new method. © 2008 Wiley Periodicals, Inc.
Transition covering tests for systems with queues
Authors: Huo Jiale , Petrenko Alexandre 
Published: June 2008

AbstractThis paper introduces a method to cover transitions of a concurrent system under test through a context consisting of infinite‐capacity queues. Concurrent systems have many important applications, but they are very difficult and expensive to test. One of the difficulties in testing concurrent systems is caused by the fact that queues in the test context can distort the behaviour of a concurrent system under test and can cause state explosion in test derivation. The proposed method derives transition covering tests directly from the specification of a concurrent system, not its composition with queues. As transition coverage is an important industrial metric of test quality, the results of the paper have practical applications. A case study is presented to illustrate one of the applications. Copyright © 2008 John Wiley & Sons, Ltd.
Changes in signaling pathways of cell proliferation and apoptosis during NK/Ly lymphoma aging
Authors: Panchuk R.R. , Boiko N.M. , Lootsik M.D. , Stoika R.S. 
Published: June 2008

AbstractExpression of specific proteins involved in regulation of cell proliferation and apoptosis was studied at the initial (7–8 days after tumor inoculation), median (13–14 days), and terminal (20–21 days) stages of murine NK/Ly lymphoma development. Western‐blot analysis using antibodies to MEK—ERK signaling pathway, E2F‐1/2 and c‐Myc, pSTAT1, pSTAT3, pSTAT5, anti‐apoptotic Bcl‐XL and pro‐apoptotic p53 and Rb proteins, as well as active cleaved forms of caspases‐3, −6, −7, was carried out to investigate the growth and survival status of NK/Ly cells. There was a marked increase in the expression of E2F‐1/2 transcription factors, MAPK signaling cascade and c‐Myc, which suggests intensive proliferation of lymphoma cells at terminal stage of tumor development. However, cytomorphological investigation and electrophoretic study of DNA fragmentation have shown degeneration of NK/Ly lymphoma cells and increase in their death. No expression of p53 protein or cleaved forms of caspases‐3, −6, −7 was found, which suggests a caspase‐independent type of apoptosis in these cells. Ascitic fluid collected at a terminal stage of NK/Ly lymphoma development was significantly weaker in supporting tumor cell growth than ascitic fluid collected at the initial stage of tumor development. It is suggested that uncontrolled cell proliferation at terminal stage of the NK/Ly lymphoma development causes nutrient deprivation and deficiency of specific growth factors in the ascitic fluid, due to overexpression of MEK—ERK, E2F and c‐Myc, thereby leading to the induction of apoptosis.