bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reovirus Serotypes Elicit Distinctive Patterns of Recall Immunity in Humans
Authors: Douville Renée N. , Su Ruey-Chyi , Coombs Kevin M. , Simons F. Estelle R. , HayGlass Kent T. 
Published: May 2008

ABSTRACTMammalian orthoreoviruses (reoviruses) are ubiquitous viral agents that infect cells in respiratory and enteric tracts. The frequency and nature of human cellular immunoregulatory responses against reovirus are unknown. Here we establish systems to detect and quantify reovirus-induced cytokine and chemokine recall responses using primary cultures of virus-infected peripheral blood mononuclear cells (PBMC) and two widely used reovirus serotypes, type 1 Lang (T1L) and type 3 Dearing (T3D) reexposure in vitro. In cultures from 44 healthy adults, reovirus induced exceptionally strong CD4 and CD8 T-cell-dependent gamma interferon (IFN-γ) recall responses concomitant with intense interleukin 10 (IL-10) production. These responses were elicited independently of viral replication. Surprisingly, paired analyses of subject responses to these two common serotypes revealed that while both elicit intense Th1-dominated immunity, median T3D-driven responses were 2.2-fold weaker (P= 0.0004) than those elicited by T1L. Recall responses evoked by these viral serotypes differed markedly in their mechanism of regulation. T3D IL-10 and IFN-γ responses were CD4 and CD8 dependent and blocked by interfering with CD86 costimulation but were CD80 independent. T1L responses were consistently CD28 and CD80/86 independent. Thus, despite extensive genetic and morphological similarities between reovirus serotypes, the nature and intensity of the human recall responses as well as the control mechanisms regulating them are clearly distinct.
Dysregulation of TLR3 Impairs the Innate Immune Response to West Nile Virus in the Elderly
Authors: Kong Kok-Fai , Delroux Karine , Wang Xiaomei , Qian Feng , Arjona Alvaro , Malawista Stephen E. , Fikrig Erol , Montgomery Ruth R. 
Published: May 2008

ABSTRACT
West Nile virus (WNV), a mosquito-borne flavivirus, has recently emerged in North America, and the elderly are particularly susceptible to severe neurological disease and death from infection with this virus. We have investigated the innate immune response of primary human macrophages to WNV in vitro and have found significant differences between the responsiveness of macrophages derived from younger donors and that from older donors. Binding of the glycosylated WNV envelope protein to the C-type lectin dendritic cell-specific intercellular adhesion molecule 3 (ICAM3) grabbing nonintegrin (DC-SIGN) leads to a reduction in the expression of Toll-like receptor 3 (TLR3) in macrophages from young donors via the signal transducer and activator of transcription 1 (STAT1)-mediated pathway. This signaling is impaired in the elderly, and the elevated levels of TLR3 result in an elevation of cytokine levels. This alteration of the innate immune response with aging may contribute to the permeability of the blood-brain barrier and suggests a possible mechanism for the increased severity of WNV infection in older individuals.
Dengue Virus Replicon Expressing the Nonstructural Proteins Suffices To Enhance Membrane Expression of HLA Class I and Inhibit Lysis by Human NK Cells
Authors: Hershkovitz Oren , Zilka Alon , Bar-Ilan Ahuva , Abutbul Shai , Davidson Andrew , Mazzon Michela , Kümmerer Beate M. , Monsoengo Alon , Jacobs Michael , Porgador Angel 
Published: May 2008

ABSTRACT
Many viruses escape the cellular immune response by downregulating cell surface expression of major histocompatibility complex (MHC) class I molecules. However, infection of cells with flaviviruses can upregulate the expression of these molecules. In this study we analyzed the expression of MHC class I in K562 and THP-1 human cell lines that were stably transfected with self-replicating subgenomic dengue virus RNA (replicons) and express all the dengue virus nonstructural proteins together. We show that MHC class I expression is upregulated in the dengue virus replicon-expressing cells and that the binding of natural killer (NK) inhibitory receptors to these cells is augmented. This upregulation results in reduced susceptibility of the dengue virus replicon-expressing cells to NK lysis, indicating a possible mechanism for evasion of the dengue virus from NK cell recognition. Visualizing MHC class I expression in replicon-containing K562 and THP-1 cells by confocal microscopy demonstrated aggregation of MHC class I molecules on the cell surface. Finally, replicon-expressing K562 cells manifested increased TAP (transporter associated with antigen processing) and LMP (low-molecular-mass protein) gene transcription, while replicon-expressing THP-1 cells manifested increased NF-κB activity and MHC class I transcription. We suggest that expression of dengue virus nonstructural proteins is sufficient to induce MHC class I upregulation through both TAP-dependent and -independent mechanisms. Additionally, aggregation of MHC class I molecules on the cell membrane also contributes to significantly higher binding of low-affinity NK inhibitory receptors, resulting in lower sensitivity to lysis by NK cells.
“Living apart together”: moral frictions between two coexisting organ transplantation schemes
Authors: Hilhorst M T 
Published: May 2008

Cadaveric transplantation and living transplantation exist side by side. Both practices help to alleviate organ need. They provide us with two separate moral schemes. Is it rational to keep them apart? The cadaveric system is organised along strict, impartial lines, while the living system is inherently partial and local. The ethical justification for this partial scheme seems to be that it merely supplements the cadaveric scheme: partial transplants do not come at the expense of cadaveric impartiality, but in fact significantly reduce the waiting time for patients on the list for a cadaveric transplant.This seemingly peaceful coexistence is challenged by new initiatives, among them living donation list exchange, and also the LifeSharers initiative, leading to practices that undermine cadaveric impartiality. Should we bemoan this fact, or should we move on towards a new balance in the relationship between cadaveric and living transplantation practices, towards a new moral weighing of impartial and partial values? I argue, against the background of a rapid growth of living donations, that we have good, ethical reasons—not only utilitarian ones—for giving the value of partiality a more prominent place in our policies.
Mélanges and ophiolites during the Pan-African orogeny: the case of the Bou-Azzer ophiolite suite (Morocco)
Authors: Bousquet Romain , El Mamoun Rachid , Saddiqi Omar , Goffé Bruno , Möller Andreas , Madi Atman 
Published: May 2008

Abstract
Since the discovery of ophiolite sequences, the Bou-Azzer inlier has been considered a key area for understanding the evolution of the northern margin of the West African craton during the Pan-African orogeny. For about 20 years, it had been commonly accepted that the Bou-Azzer inlier represents an accretionary mélange accreted onto the West African craton under blueschist metamorphic conditions, similar to the Franciscan Complex and the Sanbagawa facies series. This would imply that a low geothermal gradient was prevalent during the subduction of the Pan-African oceanic plate, and that the ocean was subducted with a high convergence rate. A reinvestigation of the metamorphic conditions by a thermodynamic approach shows that the ophiolite sequence of Bou-Azzer underwent HT greenschist metamorphic conditions instead of blueschist metamorphic conditions. We propose that the ophiolites of Bou-Azzer are not similar to the Sanbagawa facies series or to the Franciscan Complex, but bear similarities to the Albanian or Cyprus ophiolites, which represent dismembered ophiolite sequences overprinted by greenschist conditions.
Late Neoproterozoic carbonate productivity in a rifting context: the Adoudou Formation and its associated bimodal volcanism onlapping the western Saghro inlier, Morocco
Authors: Álvaro J. Javier , Macouin Mélina , Ezzouhairi Hassan , Charif A. , Ayad N. Ait , Ribeiro M. Luisa , Ader Magali 
Published: May 2008

Abstract

An interval of episodic carbonate productivity, lithostratigraphically recognized as the ‘Calcaires inférieurs’ (upper member of the Adoudou Formation), took place across the Neoproterozoic–Cambrian transition onlapping the western Saghro inlier, Morocco. Sedimentation of the ‘Calcaires inférieurs’ was highly variable: in relatively stable substrates, a peritidal-dominated mixed platform is recorded where deposition was primarily controlled by autocyclic processes and accommodation space availability, whereas, in unstable substrates, the tectonic activity associated with the inherited block-faulting basement led to deposition of complex slide sheets composed of penecontemporaneous isoclinal folds and disrupted strata. The uppermost part of the ‘Calcaires inférieurs’ displays a negative δ
13
C shift reaching values of −6.5‰. This shift may represent the δ
13
C excursion to −6‰ that marks the Neoproterozoic–Cambrian boundary in the western Anti-Atlas. Two volcanic episodes bracketed the carbonate productivity. They consist of lower basaltic flows and an upper rhyolitic ignimbrite, with a SiO
2
gap between 52 and 74 wt%. The basic rocks resemble those of tholeiitic magmas in continental rifts. The felsic rocks show high light to heavy rare earth element abundances and negative Nb, Ta, P and Ti anomalies, and were probably generated as a result of either fractional crystallization coupled with relative crustal contamination, or from a different magmatic source. The lower basic flows of tholeiitic affinity predated and geochemically differ from the alkaline magmatism of the Alougoum volcanic complex (Boho jbel) that surrounds the neighbouring Bou-Azzer inlier.
REE patterns, Nd-Sm and U-Pb ages of the metamorphic rocks of the Diagorou-Darbani greenstone belt (Liptako, SW Niger): implication for Birimian (Palaeoproterozoic) crustal genesis
Authors: Soumaila A. , Henry P. , Garba Z. , Rossi M. 
Published: May 2008

Abstract

The Palaeoproterozoic Diagorou–Darbani greenstone belt in Liptako (Niger) is made up of micaschists, various amphibolites, metaconglomerates, and metabasalts intruded by granodioritic plutons. One of these plutons, the Dargol granodiorite, is dated at 2174±4 Ma, this age is comparable with those previously reported by many researchers. The micaschists (Type 1 sediments) and intercalated amphibolites have REE patterns variously enriched in light REE (LREE), suggesting oceanic arc-related rocks. The protolith of these micaschists have calc-alkaline affinities, with crystallization ages around 2273–2278 Ma, and T
DM
close to 2.3 Ga. This age is suggestive of an early Palaeoproterozoic magmatic event in crustal growth. The metaconglomerates (Type 2 sediments) exhibit REE patterns depleted in heavy REE (HREE) typical of tonalite–trondhjemite–granodiorite (TTG), the protolith of which may have been crystallized at 2187±55 Ma. These results, together with the earlier ones, led to a Palaeoproterozoic geodynamic model in which the crustal genesis was completely related to subduction zones, with an early Palaeoproterozoic magmatic event. Partial melting of a mantle slab generated the granitoid rocks of calc-alkaline affinities, whereas those with TTG characters could have been produced by direct partial melting of subducted oceanic crust. The crustal growth may have been the result of a continuous input of crustal materials in the interval time of 2.3–2.15 Ga, corresponding to ages recorded by various detrital zircon grains of micaschists and conglomerates.
The Anti-Atlas chain (Morocco): the southern margin of the Variscan belt along the edge of the West African craton
Authors: Soulaimani Abderrahmane , Burkhard Martin 
Published: May 2008

Abstract

Broadly synchronous circum-Atlantic Variscan–Alleghanian orogenic belts developed during the Late Palaeozoic Gondwana–Laurentia collision. In the northern part of the West African craton (WAC), the Variscan orogeny produced basement-controlled structures in the Anti-Atlas, which represents the pericratonic foreland, now located south of the Variscan domains of Morocco and north of the Mauritanides belt. New structural field observations document the strong involvement of the basement and the inversion and folding of the Palaeozoic sedimentary basins at the edge the WAC. Two contrasting domains differently responding to regional NW–SE shortening are recognized: (1) a narrow belt along the Atlantic coast characterized by thin-skinned folding and ESE-vergent thrusting (para-autochthonous Anti-Atlas); (2) a large area between the WAC
sensu stricto
and the South Atlas front showing huge basement uplifts amidst a folded Palaeozoic cover with upright polyharmonic folds (autochthonous Anti-Atlas). The structural trend of the basement inliers is inherited at least in some case from previous Proterozoic fractures. Compressional reactivations led to basement uplift and concomitant folding of the Palaeozoic cover. Cover series are horizontally shortened by mostly upright symmetrical buckle folds of various wavelengths in response to thickness variations between abundant incompetent silt and shale horizons and rare competent carbonate and quartzite beds. Deformation is greatest near the borders of and between closely spaced basement uplifts. Regionally, deformation intensity decreases, either abruptly or progressively, towards the SE and it vanishes within the undeformed Tindouf basin.
Devonian extension of the Pan-African crust north of the West African craton, and its bearing on the Variscan foreland deformation: evidence from eastern Anti-Atlas (Morocco)
Authors: Baidder Lahssen , Raddi Youssef , Tahiri Mohamed , Michard André 
Published: May 2008

Abstract
The Anti-Atlas belt belongs to the northern fringe of the West African craton, moderately deformed during the Variscan orogeny south of the Meseta Block. Field-based investigations into the stratigraphy and structure of the Palaeozoic cover have been performed in the eastern part of Anti-Atlas, with emphasis on the Devonian terranes. The Pan-African basement, which crops out in the Ougnat massif, was fragmented into a mosaic of tilted blocks during a sequence of extensional faulting events that occurred from Cambrian to (mostly) Late Devonian times. The Devonian normal fault pattern indicates a multi-directional extension, with a dominant northward direction. The Variscan compression resulted in the inversion of the palaeofaults as strike-slip–reverse faults, the kinematics of which points to a NE-trending regional direction of shortening, probably Permian in age. The occurrence of the Late Devonian palaeofault array accounts for the thick-skinned style of the (eastern) Anti-Atlas belt. The Devonian paleogeography of the Anti-Atlas can be correlated with that of the Meseta, but the lack of any Late Devonian compressional event in the Anti-Atlas shows that the two domains were not mechanically coupled at that time.
A complex multi-chamber magmatic system beneath a late Cenozoic volcanic field: evidence from CSDs and thermobarometry of clinopyroxene from a single nephelinite flow (Djbel Saghro, Morocco)
Authors: Berger Julien , Ennih Nasser , Liégeois Jean-Paul , Nkono Collin , Mercier Jean-Claude C. , Demaiffe Daniel 
Published: May 2008

Abstract
We used quantitative textural measurement, electron microprobe microanalysis and thermobarometry on clinopyroxene from a Cenozoic pyroxene-nephelinite flow located along the northern boundary of the West African craton to decipher magma differentiation processes in underlying magma chambers. The crystal size distributions of clinopyroxene phenocrysts show straight but also curved and kinked patterns and the clinopyroxene show large compositional variations in a single flow (Mg-number 48–88). These observations are strong evidence for magma mixing between a nephelinite magma and a more differentiated phonolitic melt at depth. Detailed thermobarometry on these clinopyroxene shows that at least three magma chambers are present below Saghro and that they are emplaced at the main physical interface within the lithosphere: (1) at the crust–mantle boundary, where the mantle-derived nephelinite has been mixed with a pre-existing phonolitic magma chamber; (2) at the lower–upper crust boundary; (3) close to the surface in a sub-volcanic magma chamber. Some high-pressure phenocrysts (up to 14 kbar) have also probably crystallized within the upper lithospheric mantle. The high clinopyroxene proportion in samples from the base of the flow is thought to reflect crystal settling during cooling of the nephelinite flow at the surface.
Possible primary sources of diamond in the North African diamondiferous province
Authors: Kahoui M. , Mahdjoub Y. , Kaminsky F. V. 
Published: May 2008

Abstract
The Eglab shield is the easternmost part of the Reguibat rise, which belongs to the West African craton (WAC). It corresponds to the amalgamation of the Yetti and Eglab Palaeoproterozoic domains. These domains are separated by a mega-shear zone called the ‘Yetti–Eglab Junction’ where fieldwork has led to the discovery of kimberlite indicator minerals but no diamond. In the southwestern part of this zone, an outcrop of Archaean basement and a komatiitic–picritic dyke had been recognized. Within the Eglab shield, deep-seated lithospheric faults control emplacement of alkaline complexes, and of small circular structures made up of mafic, ultramafic and silica-undersaturated rocks. These structural zones are characterized by widespread development of dyke swarms and repeated reactivations of earlier Eburnean trends from the Neoproterozoic to Mesozoic. Accordingly, they are sites of high magmatic permeability and crustal weakness. In this study, we summarize all known earlier and newly obtained structural, geophysical, geological and geochemical data on this area. They indicate that the ‘Yetti–Eglab Junction’ has good possibilities for the finding of kimberlite or/and other diamondiferous rocks. The features of the Eglab shield provide a possible explanation for the enigmatic sources of the diamond-bearing Reggane placer deposit located at the boundary of the WAC.
First report of ultrahigh-temperature sapphirine + spinel + quartz and orthopyroxene + spinel + quartz parageneses discovered in Al–Mg granulites from the Khanfous area (In Ouzzal metacraton, Hoggar, Algeria)
Authors: Adjerid Z. , Ouzegane K. , Godard G. , Kienast J. R. 
Published: May 2008

Abstract

The Archaean to Palaeoproterozoic Khanfous area from the Archaean In Ouzzal granulite terrane (Western Hoggar, Algeria) preserves exceptional thermal-peak (1150<
T
< 1300 °C) mineral parageneses, consisting of orthopyroxene+spinel+quartz, sapphirine+spinel+quartz and sapphirine+orthopyroxene+quartz, in quartz-rich Al–Mg granulites. Reaction textures coupled with
P
–
T
FMASH pseudosections indicate that rocks experienced complex multi-stage evolution. Our results suggest that the Khanfous area, as well as the entire northern In Ouzzal metacraton, experienced ultrahigh-temperature crustal metamorphism attributed to a 2 Ga Palaeoproterozoic event, followed by exhumation along a clockwise
P
–
T
path. The extreme temperatures attained suggest delamination of the lithosphere and ascent of the asthenosphere after crustal thickening.
Imatinib Mesylate Inhibits Proliferation and Exerts an Antifibrotic Effect in Human Breast Stroma Fibroblasts
Authors: Gioni Vassiliki , Karampinas Theodoros , Voutsinas Gerassimos , Roussidis Andreas E. , Papadopoulos Savvas , Karamanos Nikos K. , Kletsas Dimitris 
Published: May 2008

Abstract
Tumor stroma plays an important role in cancer development. In a variety of tumors, such as breast carcinomas, a desmoplastic response, characterized by stromal fibroblast and collagen accumulation, is observed having synergistic effects on tumor progression. However, the effect of known anticancer drugs on stromal cells has not been thoroughly investigated. Imatinib mesylate is a selective inhibitor of several protein tyrosine kinases, including the receptor of platelet-derived growth factor, an important mediator of desmoplasia. Recently, we have shown that imatinib inhibits the growth and invasiveness of human epithelial breast cancer cells. Here, we studied the effect of imatinib on the proliferation and collagen accumulation in breast stromal fibroblasts. We have shown that it blocks the activation of the extracellular signal-regulated kinase and Akt signaling pathways and up-regulates cyclin-dependent kinase inhibitor p21WAF1, leading to the inhibition of fibroblast proliferation, by arresting them at the G0/G1 phase of the cell cycle. Imatinib inhibits more potently the platelet-derived growth factor–mediated stimulation of breast fibroblast proliferation. By using specific inhibitors, we have found that this is due to the inhibition of the Akt pathway. In addition, imatinib inhibits fibroblast-mediated collagen accumulation. Conventional and quantitative PCR analysis, as well as gelatin zymography, indicates that this is due to the down-regulation of mRNA synthesis of collagen I and collagen III—the main collagen types in breast stroma—and not to the up-regulation or activation of collagenases matrix metalloproteinase 2 and matrix metalloproteinase 9. These data indicate that imatinib has an antifibrotic effect on human breast stromal fibroblasts that may inhibit desmoplastic reaction and thus tumor progression. (Mol Cancer Res 2008;6(5):706–14)
A Suppressive Role of Mitogen Inducible Gene-2 in Mesenchymal Cancer Cell Invasion
Authors: Shi Xiaohua , Wu Chuanyue 
Published: May 2008

Abstract
Cancer cell invasion of extracellular matrix (ECM) is essential for dissemination of cancer cells and metastasis. In this study, we have investigated the role of mitogen inducible gene-2 (Mig-2, also known as kindlin-2), a focal adhesion protein whose expression is altered in several types of human cancers, in mesenchymal cancer cell invasion. Mig-2 is abundantly expressed in SK-LMS-1 leiomyosarcoma cells. The level of Mig-2, however, is considerably lower in more invasive HT-1080 fibrosarcoma cells. Overexpression of Mig-2 in HT-1080 and SK-LMS-1 cells substantially reduced their ability to invade ECM in an in vitro Matrigel invasion assay. Conversely, knockdown of Mig-2 markedly increased the invasiveness of these cells. Consistent with a suppressive role in mesenchymal cancer cell invasion, Mig-2 inhibits urokinase-type plasminogen activator (uPA) secretion and pericellular proteolysis. Overexpression of Mig-2 increased uPA accumulation at the intracellular face of cell-ECM adhesions and reduced the level of secreted uPA. Conversely, knockdown of Mig-2 reduced uPA accumulation at the intracellular face of cell-ECM adhesions and increased uPA secretion. Our results reveal an important role of Mig-2 in suppression of mesenchymal cancer cell invasion and shed new light on how altered Mig-2 expression could influence cancer cell invasion. (Mol Cancer Res 2008;6(5):715–24)
Attentional Selection of Multiple Goal Positions Before Rapid Hand Movement Sequences: An Event-related Potential Study
Authors: Baldauf Daniel , Deubel Heiner 
Published: May 2008

AbstractA dot-probe paradigm was used to provide physiological evidence for the parallel selection of multiple movement goals before rapid hand movement sequences. Participants executed a sequence of manual pointing movements to two out of three possible goal positions. During movement preparation, a task-irrelevant visual transient (a dot probe) was flashed either at one of both movement goals, or at the third, movement-irrelevant location. The results revealed that the N1 component induced by the presentation of the dot was enhanced if the dot was flashed at one of the movement goals, indicating that both target positions were attended before the initialization of the movement sequence. A second experiment showed that movement-irrelevant locations between the movement goals were not attended, suggesting that attention splits into spatially distinct foci.