bluuebunny/crossref_metadata_2025_split huggingface dataset:
The importance of mothers: The social transmission of COVID-19 vaccination attitudes and uptake
Authors: Thompson Oscar , Cristea Mioara , Tamariz Monica 
Published: March 2024

AbstractThe global fight against the COVID-19 pandemic has underscored the critical importance of widespread vaccination to mitigate the impact of the virus on public health. The current study aimed to investigate which social influences might be most important for predicting attitudes towards COVID-19 vaccination and vaccine uptake among young students in the UK. We focused on the cultural evolution and social transmission aspects, i.e., parent-to-child versus peer-to-peer, of attitudes and vaccine uptake during the COVID-19 pandemic. A sample of 192 UK students (aged 18 to 35 years old) filled in an online survey including measures for attitudes towards COVID-19 vaccination and vaccine uptake and/or intention, age, and gender. Participants were also asked about their mother’s, father’s, and best friend’s attitudes towards COVID-19 vaccination and vaccine uptake. Finally, they provided a subjective measure of the quality relationship with their parents. Overall, our results suggest that both parents and very close friends are important agents in understanding the students’ attitudes towards COVID-19 vaccination and vaccine uptake. More specifically, our findings suggest the mother’s vaccine uptake as the most salient predictor of students’ attitudes towards COVID-19 vaccination and vaccine uptake, particularly when the students disclose having a positive relationship with their parents. In cases where students’ experience negative relationship with their parents, the best friend’s vaccine uptake may supersede the mother’s influence. Despite these nuances, a general trend emerges from our data suggesting that vaccine uptake could be primarily guided by vertical transmission (i.e., parent to child). Our results have the potential to influence public health strategies, communication campaigns, and targeted interventions to enhance vaccination uptake. Identifying key social predictors can enable policymakers and health authorities to tailor vaccination promotion efforts towards mothers’ and peers’ vaccine uptake to increase overall positive attitudes and vaccine uptake among young people.
Neurodevelopmental defects in Dravet syndromeScn1a+/-mice: targeting GABA-switch rescues behavioral dysfunctions but not seizures and mortality
Authors: Pizzamiglio Lara , Capitano Fabrizio , Rusina Evgeniia , Fossati Giuliana , Menna Elisabetta , Léna Isabelle , Antonucci Flavia , Mantegazza Massimo 
Published: March 2024

AbstractDravet syndrome (DS) is a developmental and epileptic encephalopathy (DEE) caused by mutations of theSCN1Agene (NaV1.1 sodium channel) and characterized by seizures, motor disabilities and cognitive/behavioral deficits, including autistic traits. The relative role of seizures and neurodevelopmental defects in disease progression, as well as the role of the mutation in inducing early neurodevelopmental defects before symptoms’ onset, are not clear yet. A delayed switch of GABAergic transmission from excitatory to inhibitory (GABA-switch) was reported in models of DS, but its effects on the phenotype have not been investigated.Using a multi-scale approach, here we show that targeting GABA-switch with the drugs KU55933 (KU) or bumetanide (which upregulate KCC2 or inhibits NKCC1 chloride transporters, respectively) rescues social interaction deficits and reduces hyperactivity observed in P21Scn1a+/-DS mouse model. Bumetanide also improves spatial working memory defects. Importantly, neither KU nor bumetanide have effect on seizures or mortality rate. Also, we disclose early behavioral defects and delayed neurodevelopmental milestones well before seizure onset, at the beginning of NaV1.1 expression.We thus reveal that neurodevelopmental components in DS, in particular GABA switch, selectively underlie some cognitive/behavioral defects, but not seizures. Our work provides further evidence that seizures and neuropsychiatric dysfunctions in DEEs can be uncoupled and can have differential pathological mechanisms. They could be treated separately with targeted pharmacological strategies.
Vitrification by high pressure freezing of a wide variety of sample using the HPM Live µ
Authors: Kodera Chie , Bret Yann , Eyraud Frederic , Heiligenstein Jérôme , Belle Martin , Heiligenstein Xavier 
Published: March 2024

AbstractThis study explores the efficacy and reliability of high-pressure freezing (HPF) as a sample preparation technique for electron microscopy (EM) analysis across a diverse range of biological samples. Utilizing the HPM Live µ technology, based on the historical hydraulic HPM010 from BalTec, we demonstrate the reliability of our industrial equipment to achieve the critical parameters necessary for vitrification. By directly measuring physical values within the HPF chamber, we ensure the proper functioning of the equipment, contributing to the technique’s reliability. A meticulous approach was adopted for each sample type, acknowledging the uniqueness of each specimen, and associating final sample analysis with its HPF curve, aiding in protocol optimization. Samples including human cell pellets, cell monolayer, mouse brain and liver biopsies,C. elegans, zebrafish, andA. thalianaroot and seedlings were processed for EM analysis following HPF. The ultrastructure of each sample type was rigorously examined, revealing homogeneous preservation and minimal ice nucleation artifacts. Challenges such as plant leaf vitrification were addressed, highlighting the importance of methodological adaptation. Overall, our findings underscore the robustness and versatility of our HPM Live µ in preserving biological ultrastructure, offering valuable insights for researchers employing EM techniques in diverse biological studies.
The role of olivary phase-locking oscillations in cerebellar sensorimotor adaptation
Authors: Luque Niceto R. , Carrillo Richard R. , Naveros Francisco , Ros Eduardo , Arleo Angelo 
Published: March 2024

AbstractThe function of the olivary nucleus is key to cerebellar adaptation as it modulates long term synaptic plasticity between parallel fibres and Purkinje cells. Here, we posit that the neural dynamics of the inferior olive (IO) network, and in particular the phase of subthreshold oscillations with respect to afferent excitatory inputs, plays a role in cerebellar sensorimotor adaptation. To test this hypothesis, we first modelled a network of 200 multi-compartment Hodgkin-Huxley IO cells, electrically coupled via anisotropic gap junctions. The model IO neural dynamics captured the properties of real olivary activity in terms of subthreshold oscillations and spike burst responses to dendritic input currents. Then, we integrated the IO network into a large-scale olivo-cerebellar model to study vestibular ocular reflex (VOR) adaptation. VOR produces eye movements contralateral to head motion to stabilise the image on the retina. Hence, studying cerebellar-dependent VOR adaptation provided insights into the functional interplay between olivary subthreshold oscillations and responses to retinal slips (i.e., image movements triggering optokinetic adaptation). Our results showed that the phase-locking of IO subthreshold oscillations to retina slip signals is a necessary condition for cerebellar VOR learning. We also found that phase-locking makes the transmission of IO spike bursts to Purkinje cells more informative with respect to the variable amplitude of retina slip errors. Finally, our results showed that the joint action of IO phase-locking and cerebellar nuclei GABAergic modulation of IO cells’ electrical coupling is crucial to increase the state variability of the IO network, which significantly improves cerebellar adaptation.Author summaryThis study aims to elucidate the dual functionality of the inferior olive (IO) in cerebellar motor control, reconciling hypotheses regarding its role as either a timing or instructive signal. Specifically, we explore the role of subthreshold oscillations (STOs) within the IO, investigating their potential influence on the climbing fibres-to-Purkinje cell spike pattern responses and subsequent cerebellar adaptation, notably during the vestibulo ocular reflex. Aiming these objectives, we constructed a detailed olivary network model within a cerebellar neural network, enabling a mechanistic analysis of the functional relevance of STOs in spike burst generation, propagation, and modulation within target Purkinje cells. Our findings reveal the intricate nature of complex spike bursts triggered by climbing fibres—IO axons—into Purkinje cell dendrites, demonstrating a hybrid nature involving binary clock-like signals and graded spikelet components acting as an instructive signal.
Human immunodeficiency virus-1 induces host genomic R-loop and preferentially integrates its genome near the R-loop regions
Authors: Park Kiwon , Lee Dohoon , Jeong Jiseok , Lee Sungwon , Kim Sun , Ahn Kwangseog 
Published: March 2024

AbstractAlthough HIV-1 integration sites favor active transcription units in the human genome, high-resolution analysis of individual HIV-1 integration sites has shown that the virus can integrate into a variety of host genomic locations, including non-genic regions. The invisible infection by HIV-1 integrating into non-genic regions, challenging the traditional understanding of HIV-1 integration site selection, is more problematic because they are selected for preservation in the host genome during prolonged antiretroviral therapies. Here, we showed that HIV-1 integrates its viral genome into the vicinity of R-loops, a genomic structure composed of DNA– RNA hybrids. VSV-G-pseudotyped HIV-1 infection initiates the formation of R-loops in both genic and non-genic regions of the host genome and preferentially integrates into R-loop-rich regions. Using a HeLa cell model that can independently control transcriptional activity and R-loop formation, we demonstrated that the exogenous formation of R-loops directs HIV-1 integration-targeting sites. We also found that HIV-1 integrase proteins physically bind to the host genomic R-loops. These findings provide novel insights into the mechanisms underlying retroviral integration and the new strategies for antiretroviral therapy against HIV-1 latent infection.
Ectopic DNA integration and marker-free CRISPR/Cas9 strategies for the halotolerant black yeastHortaea werneckii
Authors: Hernandez-Rodriguez Yainitza , Bullard A. Makenzie , Busch Rebecca J. , Marshall Aidan , Vargas-Muñiz José M. 
Published: March 2024

AbstractHortaea werneckiiis a halotolerant black yeast commonly found in hypersaline environments. This yeast is also the causative agent of tinea nigra, a superficial mycosis of the palm of the hand and soles of the feet of humans. In addition to their remarkable halotolerance, this black yeast exhibits an unconventional cell division cycle, alternating between fission and budding cell division. Cell density and the salt concentration in their environment regulate which cell division cycleH. werneckiiuses. AlthoughH. werneckiihave been extensively studied due to their unique physiology and cell biology, deciphering the underlying mechanisms behind these remarkable phenotypes has been limited due to the lack of genetic tools available. Here, we report a new ectopic integration protocol forH. werneckiiusing PEG-CaCl2mediated protoplast transformation. This approach relies on a drug (hygromycin B) resistance gene to select for successful integration of the genetic construct. The same construct was used to express cytosolic green fluorescent protein. Finally, we developed a marker-free CRISPR/Cas9 protocol for targeted gene deletion using the melanin synthesis pathway as a visual reporter of successful transformation. These transformation strategies will allow testing hypotheses related toH. werneckiicell biology and physiology.ImportanceHortaea werneckiiis a remarkable yeast capable of growing in high salt concentration, and its cell division cycle alternates between fission-like and budding. For these unique attributes,H. werneckiihas gathered interest in a research program studying extremophile fungi and cell division. Most of our understanding ofH. werneckiibiology comes from genomic analyses, usage of drugs to target a particular pathway or heterologous expression of its gene inS. cerevisiae. Nonetheless,H. werneckiihas remained genetically intractable. Here, we report on two strategies to transformH. werneckii: ectopic integration of a plasmid and gene deletion using CRISPR/Cas9. These approaches will be fundamental to expanding the experimental techniques available to studyH. werneckii, including live cell imaging of cellular processes and reverse genetic approaches.
Centriole structural integrity defects are a crucial feature of Hydrolethalus Syndrome
Authors: Curinha Ana , Huang Zhaoyu , Anglen Taylor , Strong Margaret A. , Gliech Colin R. , Jewett Cayla E. , Friskes Anoek , Holland Andrew J. 
Published: March 2024

ABSTRACTHydrolethalus Syndrome (HLS) is a lethal, autosomal recessive ciliopathy caused by the mutation of the conserved centriole protein HYLS1. However, how HYLS1 facilitates the centriole-based templating of cilia is poorly understood. Here, we show that mice harboring the HYLS1 disease mutation die shortly after birth and exhibit developmental defects that recapitulate several manifestations of the human disease. These phenotypes arise from tissue-specific defects in cilia assembly and function caused by a loss of centriole integrity. We show that HYLS1 is recruited to the centriole by CEP120 and functions to recruit centriole inner scaffold proteins that stabilize the centriolar microtubule wall. The HLS mutation disrupts the interaction of HYLS1 with CEP120 leading to HYLS1 displacement and degeneration of the centriole distal end. We propose that tissue-specific defects in centriole integrity caused by the HYLS1 mutation prevent ciliogenesis and drive HLS phenotypes.
CD47 is Required for Mesenchymal Progenitor Proliferation and Fracture Repair
Authors: Zondervan Robert L. , Capobianco Christina A. , Jenkins Daniel C. , Reicha John D. , Fredrick Livia M. , Lam Charles , Isenberg Jeffery S. , Ahn Jaimo , Marcucio Ralph S. , Hankenson Kurt D. 
Published: March 2024

AbstractCD47 is a ubiquitous and pleiotropic cell-surface receptor. Disrupting CD47 enhances injury repair in various tissues but the role of CD47 has not been studied in bone injuries. In a murine closed-fracture model, CD47-null mice showed decreased callus bone volume, bone mineral content, and tissue mineral content as assessed by microcomputed tomography 10 days post-fracture, and increased fibrous volume as determined by histology. To understand the cellular basis for this phenotype, mesenchymal progenitors (MSC) were harvested from bone marrow. CD47-null MSC showed decreased large fibroblast colony formation (CFU-F), significantly less proliferation, and fewer cells in S-phase, although osteoblast differentiation was unaffected. However, consistent with prior research, CD47-null endothelial cells showed increased proliferation relative to WT cells. Similarly, in a murine ischemic fracture model, CD47-null mice showed reduced fracture callus bone volume and bone mineral content relative to WT. Consistent with ourin vitroresults,in vivoEdU labeling showed decreased cell proliferation in the callus of CD47-null mice, while staining for CD31 and endomucin demonstrated increased endothelial cell mass. Finally, WT mice administered a CD47 morpholino, which blocks CD47 protein production, showed a callus phenotype similar to that of non-ischemic and ischemic fractures in CD47-null mice, suggesting the phenotype was not due to developmental changes in the knockout mice. Thus, inhibition of CD47 during bone healing reduces both non-ischemic and ischemic fracture healing, in part, by decreasing MSC proliferation. Furthermore, the increase in endothelial cell proliferation and early blood vessel density caused by CD47 disruption is not sufficient to overcome MSC dysfunction.
Cryo-EM structure of the tetra-phosphorylated R-domain in Ycf1 reveals key interactions for transport regulation
Authors: de Carvalho Rodolpho S. A. , Rasel Md Shamiul I. , Khandelwal Nitesh K. , Tomasiak Thomas M. 
Published: March 2024

AbstractMany ATP-binding cassette (ABC) transporters are regulated by phosphorylation on long and disordered loops which present a challenge to visualize with structural methods. We have trapped an activated state of the regulatory domain (R-domain) of Yeast Cadmium Factor 1 (Ycf1) by enzymatically enriching the phosphorylated state. A 3.2 Å cryo-EM structure reveals an R-domain structure with four phosphorylated residues and a position for the entire R-domain. The structure reveals key R-domain interactions including a bridging interaction between NBD1 and NBD2 as well as an interaction with the R-insertion, another regulatory region. We systematically probe these interactions with a linker substitution strategy along the R-domain and find a close match with these interactions and survival under Ycf1-dependent growth conditions. We propose a model where four overlapping phosphorylation sites bridge several regions of Ycf1 to engage in a transport-competent state.
Breaking the institutional logic or going under - Controllers and sustainability
Authors: Kunz Jennifer , Oltmann Johanna , Weinhart Felix 
Published: March 2024

PurposeThe present paper aims to focus on the role which German controllers play so far in the process of sustainable transformation in for-profit organizations, the current obstacles to a wider engagement here and ways to overcome these obstacles.Design/methodology/approachThe analysis combines two qualitative study designs. Empirical data is generated via a job advertisement analysis and an explorative survey with 107 subjects from management accounting/controlling and sustainability management. The generated data is interpreted against the background of the theory of institutional logics and Abbott’s (1988) theory of professional jurisdiction.FindingsWe find that controllers are in a state of tension. On the one hand, the pressure to integrate sustainability into companies is increasing. On the other hand, they seem to be rather reluctant to get involved. The institutional logics that shape their profession play an important role here, as does an unclear relationship with the sustainability department, which has its own claims here. Based on these observations, we identify the core obstacles to the transformation of the controllers’ profession and discuss solutions which can guide the transformation of this profession.Originality/valueThe present paper provides insights from a unique combination of different quantitative study designs and different perspectives on the possible role that controllers can play in advancing sustainable transformation in companies.
Enhancing green service innovation behavior through green involvement: the role of information technology adoption
Authors: Tseng Shu-Mei , Octavyaputri Shervina 
Published: March 2024

Purpose Developing green innovative services is critical to the restaurant industry to achieve significant benefits as well as environmental sustainability. This study aims to explore the mechanisms through which employees’ green involvement can foster green service innovation behavior.Design/methodology/approach The data set garnered from employees who worked in restaurants was used to test these mechanisms. A partial least square technique was conducted on this data set.Findings The results revealed the employees’ green involvement significantly influences their green service innovation intention, which subsequently influences their green service innovation behavior. Furthermore, information technology (IT) adoption was found to fortify the linkage of employee green involvement with green service innovation intention.Practical implications The results suggest to the restaurant industry that awareness of green service innovation and IT adoption practices can help restaurants to develop effective sustainability work practices and meet societal expectations.Originality/value This study extends the restaurant management literature by linking the green involvement of restaurant employees to green service innovation intention as well as identifying the moderating role of IT adoption underlying this link.
Performance analysis of columnar convex-concave compound microtexture thrust bearing
Authors: Wang Lili , Liu Ying’ao , Duan Jingdong , Bao Yunlong 
Published: March 2024

Purpose
This study aims to enhance the lubrication performance of thrust bearings. The influence of columnar convex–concave compound microtexture on bearing performance is investigated

Design/methodology/approach
Based on the compound microtexture model of thrust bearings, considering surface roughness and turbulent effect, the variation of lubrication characteristics with the change in the compound microtexture parameters is studied.

Findings
The results indicate that, compared with circular microtexture, the maximum pressure of compound microtexture of thrust bearings increases by 7.42%. Optimal bearing performance is achieved when the internal microtexture depth is 0.02 mm. Turbulent flow states and surface roughness lead to a reduction in the optimal depth. The maximum pressure and load-carrying capacity of the bearing decrease as the initial angle increases, whereas the friction coefficient increases with the increase in the initial angle. The lubrication performance is best for bearings with a circumferential parallel arrangement of microtexture.

Originality/value
The novel composite microtexture with columnar convex-concave is proposed, and the computational model of thrust bearings is set. The influence of surface roughness and turbulent flow on the bearing performance should be considered for better conforming with engineering practice. The effect of microtexture depth, arrangement method and distribution position on the lubrication performance of the compound microtexture thrust bearing is investigated, which is of great significance for improving tribology, thrust bearings and surface microtexture theory.
Collaborative federated learning framework to minimize data transmission for AI-enabled video surveillance
Authors: Sugianto Nehemia , Tjondronegoro Dian , Sorwar Golam 
Published: March 2024

PurposeThis study proposes a collaborative federated learning (CFL) framework to address personal data transmission and retention issues for artificial intelligence (AI)-enabled video surveillance in public spaces.Design/methodology/approachThis study examines specific challenges for long-term people monitoring in public spaces and defines AI-enabled video surveillance requirements. Based on the requirements, this study proposes a CFL framework to gradually adapt AI models’ knowledge while reducing personal data transmission and retention. The framework uses three different federated learning strategies to rapidly learn from different new data sources while minimizing personal data transmission and retention to a central machine.FindingsThe findings confirm that the proposed CFL framework can help minimize the use of personal data without compromising the AI model's performance. The gradual learning strategies help develop AI-enabled video surveillance that continuously adapts for long-term deployment in public spaces.Originality/valueThis study makes two specific contributions to advance the development of AI-enabled video surveillance in public spaces. First, it examines specific challenges for long-term people monitoring in public spaces and defines AI-enabled video surveillance requirements. Second, it proposes a CFL framework to minimize data transmission and retention for AI-enabled video surveillance. The study provides comprehensive experimental results to evaluate the effectiveness of the proposed framework in the context of facial expression recognition (FER) which involves large-scale datasets.