bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel distribution of junctional adhesion molecule‐C in the neural retina and retinal pigment epithelium
Authors: Daniele Lauren L. , Adams Ralf H. , Durante Diane E. , Pugh Edward N. , Philp Nancy J. 
Published: September 2007

AbstractJunction adhesion molecules‐A, ‐B, and ‐C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam‐C mRNA was increased threefold in the all‐cone retina of the Nrl−/− mouse, suggesting that Jam‐C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam‐C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between Müller and photoreceptor cells. Additionally, Jam‐C labeling was observed in the long apical processes of Müller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam‐B was also detected at the OLM. In the developing retina, Jam‐B and ‐C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam‐C−/− mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam‐A was not detected at the OLM in wild‐type retinas, it was present at the OLM in retinas of Jam‐C−/− mice. These findings indicate that up‐regulation of Jam‐A in the retina compensates for the loss of Jam‐C. The nonclassical distribution of Jam‐C in the apical membranes of Müller cells and RPE suggests that Jam‐C has a novel function in the retina. J. Comp. Neurol. 505:166–176, 2007. © 2007 Wiley‐Liss, Inc.
Numerical solution of a mathematical model for capillary formation in tumor angiogenesis via the tau method
Authors: Saadatmandi Abbas , Dehghan Mehdi 
Published: September 2007

AbstractA numerical procedure is developed to obtain the solution of a mathematical model for capillary formation in tumor angiogenesis. The proposed method is based on the shifted Legendre tau technique. Our approach consists of reducing the problem to a set of algebraic equations by expanding the approximate solution as a shifted Legendre polynomial with unknown coefficients. The operational matrices of integral and derivative together with the tau method are then utilized to evaluate the unknown coefficients of shifted Legendre polynomials. Illustrative example is included to demonstrate the validity and applicability of the presented technique. Copyright © 2007 John Wiley & Sons, Ltd.
Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries
Authors: Levering Wilfried H. B. M. , van Wieringen Wessel N. , Kraan Jaco , van Beers Wil A. M. , Sintnicolaas Kees , van Rhenen Dick J. , Gratama Jan W. 
Published: September 2007

AbstractA biannual external quality assessment (EQA) scheme for flow cytometric lymphocyte immunophenotyping is operational in the Benelux countries since 1996. We studied the effects of the methods used on assay outcome, and whether or not this EQA exercise was effective in reducing between‐laboratory variation. Eighty test samples were distributed in 20 biannual send‐outs. Per send‐out, 50–71 participants were requested to enumerate CD3+, CD4+, and CD8+T cells, B cells, and NK cells, and to provide methodological details. Participants received written debriefings with personalized recommendations after each send‐out. For this report, data were analyzed using robust multivariate regression. Five variables were associated with significant positive or negative bias of absolute lymphocyte subset counts: (i) platform methodology (i.e., single‐platform assays yielded lower CD4+and CD8+T‐cell counts than did dual‐platform assays); (ii) sample preparation technique (i.e., assays based on mononuclear cells isolation yielded lower T‐cell counts than those based on red cell lysis); (iii) gating strategies based on CD45 and sideward scatter gating of lymphocytes yielded higher CD4+T‐cell counts than those based on “backgating” of lymphocytes guided by CD45 and CD14); (iv) stabilized samples were generally associated with higher lymphocyte subset counts than nonstabilized samples; and (v) laboratory. Platform methodology, sample stabilization, and laboratory also affected assay variability. With time, assay variability tended to decline; this trend was significant for B‐cell counts only. In addition, significant bias and variability of results, independent of the variables tested for in this analysis, were also associated with individual laboratories. In spite of our recommendations, participants tended to standardize their techniques mainly with respect to sample preparation and gating strategies, but less with absolute counting techniques. Failure to fully standardize protocols may have led to only modest reductions in variability of results between laboratories. © 2007 Clinical Cytometry Society.
TLR agonists induce the differentiation of human bone marrow CD34+ progenitors into CD11c+ CD80/86+ DC capable of inducing a Th1‐type response
Authors: Sioud Mouldy , Fløisand Yngvar 
Published: September 2007

AbstractWe recently reported that human bone marrow hematopoietic CD34+ progenitors express functional Toll‐like receptors (TLR) and can differentiate into myeloid cells just by stimulation with resiquimod (R848), a specific agonist for TLR7/8. However, the mechanisms by which R848 induces cell differentiation, the effects of other TLR agonists and the functionality of the differentiated cells are not known. Comparable to R848, loxoribine (a TLR7 agonist) and Pam3CSK4 (a TLR2 agonist) induced cytokine production and cell differentiation along the myeloid lineage. R848 and loxoribine were more effective than Pam3CSK4 at inducing the lineage‐negative (CD11c+ CD14–) dendritic cells (DC), whereas Pam3CSK4 was more effective at inducing CD11c+ CD14+ monocytes. Both cell subsets expressed CD80/CD86 and HLA‐DR molecules; however, they showed differential expression of CD1a, CD1b, CD1c, CD11b, CD206 and CD207 markers when compared with each other. Cell differentiation into DC was significantly inhibited by an anti‐TNF‐α nonoclonal antibody. The CD11c+ CD14– subset was isolated and shown to be more potent in stimulating an alloreaction than the CD11c+ CD14+ subset. Collectively, these data highlight the differential effects of TLR agonists on human bone marow CD34+ progenitor cells and provide a new opportunity for generating functional DC that would be useful in cancer vaccination.
Up‐regulation of leukocyte CXCR4 expression by sulfatide: An L‐selectin‐dependent pathway on CD4+ T cells
Authors: Duchesneau Pascal , Gallagher Erin , Walcheck Bruce , Waddell Thomas K. 
Published: September 2007

AbstractCXCR4 plays significant roles in immune and inflammatory responses and is important for selective recruitment of leukocytes. We previously showed that CXCR4 surface expression of human lymphocytes was affected by sulfatide, an in vivo ligand for L‐selectin. Increased CXCR4 expression was shown to promote biologically relevant functions such as integrin‐dependent adhesion and transmigration. Here, we show that sulfatide‐induced CXCR4 up‐regulation also occurs on other leukocyte subsets in humans and mice. B cells and CD4+CD25+ T cells had the highest CXCR4 up‐regulation after sulfatide stimulation. Transfection of L‐selectin was sufficient for K562 cells to acquire sulfatide‐induced CXCR4 up‐regulation, while analysis of L‐selectin knockout mice revealed that this response was critically L‐selectin dependent only for CD4+ T cells, suggesting an alternative pathway in CD8+ T cells and B cells. Sulfatide triggered several intracellular signaling events in CD4+ T cells, but only tyrosine kinase activation, including members of the Src family, were essential for L‐selectin to CXCR4 signaling. CXCR4 up‐regulation was rapid, enhanced CXCL12‐induced signaling and increased chemotaxis toward CXCL12, and therefore has potentially important roles in vivo. Thus, the response to CXCL12 depends in part on tissue expression of sulfatide and, specifically in CD4+ T cells, also depends on the surface level of L‐selectin.
MyD88‐dependent autoimmune disease in Lyn‐deficient mice
Authors: Silver Karlee L. , Crockford Tanya L. , Bouriez‐Jones Tiphaine , Milling Simon , Lambe Teresa , Cornall Richard J. 
Published: September 2007

AbstractRecent evidence suggests that systemic autoimmune disease depends on signals from TLR ligands, but little is known about how TLR‐dependent pathways lead to the loss of self tolerance in vivo. To address this, we have examined the role of TLR signaling in Lyn‐deficient mice, which develop an autoimmune disease similar to SLE. We found that absence of the TLR signaling adaptor molecule MyD88 suppresses plasma cell differentiation of switched and unswitched B cells, and prevents the generation of antinuclear IgG antibodies and glomerulonephritis. In mixed chimeras the increased IgM and IgG antibody secretion in Lyn‐deficient mice is at least partially due to B cell‐independent effects of Lyn. We now show that MyD88 deficiency blocks the expansion and activation of DC in which Lyn is also normally expressed, and prevents the hypersecretion of proinflammatory cytokines IL‐6 and IL‐12 by Lyn‐deficient DC. These findings further highlight the important role of TLR‐dependent signals in both lymphocyte activation and autoimmune pathogenesis.
TLR9 activation is a key event for the maintenance of a mycobacterial antigen‐elicited pulmonary granulomatous response
Authors: Ito Toshihiro , Schaller Matthew , Hogaboam Cory M. , Standiford Theodore J. , Chensue Stephen W. , Kunkel Steven L. 
Published: September 2007

AbstractType 1 (Th1) granulomas can be studied in mice sensitized with mycobacterium antigens followed by challenge of agarose beads covalently coupled to purified protein derivative. TLR9 is known to play a role in the regulation of Th1 responses; thus, we investigated the role of TLR9 in granuloma formation during challenge with mycobacterium antigens and demonstrated that mice deficient in TLR9 had increased granuloma formation, but a dramatically altered cytokine phenotype. Th1 cytokine levels of IFN‐γ and IL‐12 in the lungs were decreased in TLR9–/– mice when compared to wild‐type mice. In contrast, Th2 cytokine levels of IL‐4, IL‐5, and IL‐13 were increased in TLR9–/– mice. The migration of CD4+ T cells in the granuloma was impaired, while the number of F4/80+ macrophages was increased in TLR9–/– mice. Macrophages in the lungs of the TLR9‐deficient animals with developing granulomas expressed significantly lower levels of the classically activated macrophage marker, nitric oxide synthase, but higher levels of the alternatively activated macrophage markers such as ‘found in inflammatory zone‐1′ antigen and Arginase‐1. These results suggest that TLR9 plays an important role in maintaining the appropriate phenotype in a Th1 granulomatous response.
Square‐Planar Diacetatopalladium Complexes with trans‐Configured Secondary Amine Ligands that Avoid Orthometalation: Ligand Synthesis, Coordination, Molecular Structure and Catalytic Potential for Suzuki Cross‐Coupling Reactions
Authors: Chahen Ludovic , Therrien Bruno , Süss‐Fink Georg 
Published: September 2007

AbstractThe trans‐configured square‐planar palladium complexes [Pd(OAc)2(LNtBu)2] (1), [Pd(OAc)2(η2‐LN∩NtBu)] (2), [Pd(OAc)2(LNPh)2] (3), and [Pd(OAc)2(η2‐LN∩NPh)] (4), have been synthesized by treating palladium acetate with the amines NHtBuCH2‐2,4,6‐Me3C6H2 (LNtBu) or NHPhCH2‐2,4,6‐Me3C6H2 (LNPh) or with the diamines NHtBuCH2‐2,4,6‐Me3C6H‐CH2‐2,4,6‐Me3C6H‐CH2‐NHtBu (LN∩NtBu) or NHPh‐CH2‐2,4,6‐Me3C6H‐CH2‐2,4,6‐Me3C6H‐CH2‐NHPh (LN∩NPh). The single‐crystal X‐ray structure analysis of complexes 1–3 confirms a trans arrangement of the two acetato groups and of the two nitrogen atoms. Orthometalation leading to palladacycles is impossible in all cases as the ortho positions in the benzylic rings are blocked by methyl substituents. All complexes are found to catalyze Suzuki cross‐coupling reactions of deactivated and even sterically hindered arene substrates. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Cyclodiphosphazane cis‐{(o‐MeOC6H4O)P(μ‐NtBu)}2 as a Bridging Bidentate Ligand: Synthesis, Structures of Heterometallic Complexes, and Halogen Exchange Between Rh–Cl and Cu–X (X = Br, I)
Authors: Chandrasekaran P. , Mague Joel T. , Venkateswaran Ramalingam , Balakrishna Maravanji S. 
Published: September 2007

AbstractThe mononuclear rhodium complexes of cyclodiphosphazane [(cod)RhCl{L‐κP}] (1a) (L = cis‐{(o‐MeOC6H4O)P(μ‐NtBu)}2) and trans‐[Rh(CO)Cl{L‐κP}2] (1b) react in a 1:1 molar ratio with [AuCl(SMe2)] to form the heteronuclear complexes [(cod)RhCl{μ‐L‐κP,κP}AuCl] (2) and trans‐[Rh(CO)Cl{μ‐L‐κP,κP}2(AuCl)2] (7), respectively, in quantitative yield. The reaction between 1a and CuCl afforded a tetranuclear complex [(cod)RhCl{μ‐L‐κP,κP}Cu(μ‐Cl)]2 (3). Under similar reaction conditions 1 reacts with 2 equiv. of CuX (X = Br, I) to produce the heteronuclear complexes [(cod)RhBr{μ‐L‐κP,κP}Cu(μ‐Br)]2 (4) and [((cod)RhI{μ‐L‐κP,κP})2Cu(μ‐X)2Cu] (X ≈ 1:1 mixture of Cl and I disordered over both sites) (5), respectively, in which two molecules of 1a are bridged by the rhombic, disordered, mixed halide [Cu(μ‐X)2Cu] (5: X = Br; 6: X = Cl, I) unit. The crystal structures of 4 and 5 confirm the halogen exchange between the Rh–Cl and CuX (X = Br, I) moieties which leads to the formation of Rh–Br and Rh–I bonds. The heterodinuclear RhI/PdII complex [(cod)RhCl{μ‐L‐κP,κP}PdCl(η3‐C3H5)] (6) was synthesized by the reaction of 1a with [PdCl(η3‐C3H5)]2 in a 2:1 ratio. Reaction of 1b with CuI leads to the formation of the tetranuclear complex [Rh(CO)I{μ‐L‐κP,κP}2Cu(μ‐I)]2 (8) containing two terminal uncoordinated phosphorus(III) centers irrespective of reactant ratio and reaction conditions. Halogen exchange reaction which leads to the formation of the Rh–I bond was also observed during the preparation of complex 8. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Expanding the Range of “Daniphos”‐Type P∩P‐ and P∩N‐Ligands: Synthesis and Structural Characterisation of New [(η6‐arene)Cr(CO)3] Complexes
Authors: Alberico Elisabetta , Braun Wolfgang , Calmuschi‐Cula Beatrice , Englert Ulli , Salzer Albrecht , Totev Daniel 
Published: September 2007

AbstractNew P∩P‐ and P∩N‐ligands have been synthesised whose core structure is an [(η6‐arene)Cr(CO)3] unit. These new ligands, which extend the range of “Daniphos” ligands, are endowed with central and planar chirality and have been prepared through a stereoselective synthetic strategy from optically pure benzylamines bearing a second substituent on the arene other than the benzyldimethylamino group. Because the two faces of unsymmetrically 1,2‐ and 1,3‐disubstituted benzylamine are diastereotopic, which means that diastereomeric complexes arise upon coordination of theCr(CO)3 fragment to either of these two faces, the synthetic plan has been adjusted by exploiting the trimethylsilyl group as a temporary steric modulator in order to access both complexes with high diastereoselectivity. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Formation of Boronate Ester Polymers with Efficient Intrastrand Charge‐Transfer Transitions by Three‐Component Reactions
Authors: Christinat Nicolas , Croisier Emmanuel , Scopelliti Rosario , Cascella Michele , Röthlisberger Ursula , Severin Kay 
Published: September 2007

AbstractThe three‐component reaction of aryl boronic acids with 1,2,4,5‐tetrahydroxybenzene and 1,2‐bis(4‐pyridyl)ethylene or 4,4′‐bipyridine leads to the formation of dark‐purple boronate ester polymers. Crystallographic analyses show that the polymer strands have a zig–zag geometry, and the bis(dioxaborole) units are connected by dipyridyl linkers through dative B–N interactions. Upon dissolution of the polymers in hot chloroform, most of the B–N connections are broken, which indicates that polymer formation is a reversible process. A computational study provides evidence that the strong color of the polymers is due to efficient intrastrand charge‐transfer excitations from the tetraoxobenzene to the dipyridyl linker.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Palladium‐Catalyzed Alkynylcarbonylation of Aryl Iodides with the Use of Mo(CO)6 in the Presence of tBu3P Ligand
Authors: Iizuka Muneaki , Kondo Yoshinori 
Published: September 2007

AbstractPalladium‐catalyzed alkynylcarbonylation of aryl iodides was accomplished by using Mo(CO)6 as a CO source. The reaction was conducted at room temperature with the use of tBu3P as a ligand, which was found to be essential for smooth carbonylation. One‐pot construction of pyrazoles by using aryl iodides with electron‐withdrawing groups was also accomplished under these reaction conditions in the presence of methylhydrazine. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Palladium Biosensor
Authors: Adam Vojtech , Hanustiak Pavel , Krizkova Sona , Beklova Miroslava , Zehnalek Josef , Trnkova Libuse , Horna Ales , Sures Bernd , Kizek Rene 
Published: September 2007

AbstractIn this paper we proposed a palladium(II) biosensor. The biosensor is based on determining of interactions between palladium(II) and metallothionein modified hanging mercury drop electrode by means of differential pulse voltammetry. We studied influence of two supporting electrolytes (potassium or sodium chloride) on the signals of the biosensor. Based on the results obtained we found potassium chloride (0.05 M) as the most suitable supporting electrolyte to determine palladium(II). The detection limit of the biosensor for palladium ions was evaluated as 100 nM with RSD about 10%. Moreover, we utilized the biosensor for measurement of the target molecule in the presence of human blood serum and human urine.
Square‐Wave Voltammetry Applied to the Analysis of the Dye Marker, Solvent Blue 14, in Kerosene and Fuel Alcohol
Authors: Trindade Magno Aparecido Gonçalves , Boldrin Zanoni Maria Valnice 
Published: September 2007

AbstractThe purpose of this paper is to develop an electroanalytical method based on square‐wave voltammetry (SWV) for the determination of the solvent blue 14 (SB‐14) in fuel samples. The electrochemical reduction of SB‐14 at glassy carbon electrode in a mixture of Britton‐Robinson buffer withN,N‐dimethylformamide (1 : 1, v/v) presented a well‐defined peak at−0.40 V vs. Ag/AgCl. All parameters of the SWV technique were optimized and the electroanalytical method presented a linear response from 1.0×10−6to 6.0×10−6 mol L−1(r=0.998) with a detection limit of 2.90×10−7 mol L−1. The developed method was successfully utilized in the quantification of the dye SB‐14 in kerosene and alcohol samples with average recovery from 93.00 to 98.10%.
Determination of Folic Acid by Adsorptive Stripping Voltammetry at a Lead Film Electrode
Authors: Korolczuk Mieczyslaw , Tyszczuk Katarzyna 
Published: September 2007

AbstractAn adsorptive stripping voltammetric procedure for the determination of folic acid at an in situ plated lead film electrode was described. Formation of lead film on a glassy carbon substrate and accumulation of folic acid was performed simultaneously from an acetate buffer solution of pH 5.6 at the potential −0.88 V. The measurements were carried out from aerated solutions. The calibration graph for an accumulation time of 300 s was linear from 2×10−9 to 5×10−8 mol L−1. The detection limit was 7×10−10 mol L−1, the relative standard deviation for 2×10−8 mol L−1 of folic acid was 3.9%. The proposed procedure was applied to folic acid determinations in pharmaceutical preparations.
Electrocatalysis and Amperometric Detection of the Reduced Form of Nicotinamide Adenine Dinucleotide at Toluidine Blue/Zinc Oxide Coated Electrodes
Authors: Kumar S. Ashok , Chen Shen‐Ming 
Published: September 2007

AbstractThin toluidine blue (TBO) and zinc oxide (ZnO) hybrid films have been grown on glassy carbon electrode (GCE) and indium tin oxide coated (SnO2) glass electrodes by using cyclic voltammetry (CV). Scanning electron microscopy (SEM) images revealed spherical and beads‐like shape of highly oriented TBO/ZnO hybrid films. Energy dispersive spectrometry (EDS) results declared that the films composed mainly of Zn and O. Moreover, TBO/ZnO hybrid films modified electrode is electrochemically active, dye molecules were not easily leached out from the ZnO matrix and the hybrid films can be considered for potential applications as sensor for amperometric determination of reduced nicotinamide adenine dinucleotide (NADH) at 0.0 V. A linear correlation between electrocatalytic current and NADH concentration was found to be in the range between 25 μM and 100 μM in phosphate buffer. In addition, we observed that dopamine, ascorbic acid and uric acid are not interference in amperometric detection of NADH in this proposed method. In addition, TBO/ZnO hybrid film modified electrode was highly stable and its response to the NADH also remained relentless.
Silver Amalgam Film Electrode of Prolonged Application in Stripping Chronopotentiometry
Authors: Kapturski Paweł , Bobrowski Andrzej 
Published: September 2007

AbstractThe utility of the cylindrical silver‐based mercury film electrode of prolonged analytical application in stripping chronopotentiometry (SCP) was examined. This electrode allowed us to obtain good reproducibility of results owing to the special electrode design, which enables regeneration of the thin layer before each measurement cycle. The accessible potential window in KNO3 (pH 2), acetate and ammonia buffers was defined, and the optimal conditions (i.e., stripping current, deposition potential and deposition time) for the determination of Cd and Pb traces were selected. The detection limits, obtained for an accumulation time of 60 s, were 0.023 μg/L for Cd and 0.075 μg/L for Pb. The response increases linearly with Cd, Pb and Zn concentration, up to at least 100 μg/L. It was also shown that the proposed procedure ensures excellent separation of the In and Tl, Pb and Tl or the In and Cd signals. The method was tested with dolomite and lake sediment samples, and good agreement with reference values was achieved. The obtained results showed good reproducibility (RSD=5–6%) and reliability.
A comparison of the hierarchical likelihood and Bayesian approaches to spatial epidemiological modelling
Authors: Jang Myoung Jin , Lee Youngjo , Lawson Andrew B. , Browne William J. 
Published: September 2007

AbstractRecently Bayesian methods have been widely used in disease mapping. Hierarchical (h‐) likelihood methods allow reliable likelihood inference in random‐effect models and it is therefore interesting to compare h‐likelihood and Bayesian methods. For comparison we consider three examples: low birth weight and cancer mortality data in South Carolina and lip cancer data in Scotland. Mean estimates from both h‐likelihood and Bayesian approaches are almost identical, while variance‐component estimates can be somewhat different, depending upon choice of priors. Copyright © 2007 John Wiley & Sons, Ltd.
One‐day forward premiums and the impact of virtual bidding on the New York wholesale electricity market using hourly data
Authors: Hadsell Lester , Shawky Hany A. 
Published: September 2007

AbstractThis study examines one‐day forward premiums at the hourly level on the New York independent systems operator wholesale electricity market for the period 2001–2005. Examining two representative zones, the authors show that premiums vary by hour of day, day of week, and month. We report differences in the level and volatility of the premium across zones. We measure the impact of opening the market to outsiders through the introduction of virtual bidding. Results indicate that virtual bidding is associated with lower premiums in off‐peak or near‐off‐peak hours; it is associated with higher premiums during peak hours. © 2007 Wiley Periodicals, Inc. Jrl Fut Mark 27: 1107–1125, 2007