bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional outcomes after surgical reconstruction of the base of tongue using the radial forearm free flap in patients with oropharyngeal carcinoma
Authors: Rieger Jana M. , Zalmanowitz Jana G. , Li Shirley Y. Y. , Sytsanko Anna , Harris Jeffrey , Williams David , Seikaly Hadi 
Published: April 2007

AbstractBackground:Historically, the prevention of functional morbidity after multimodal treatment for squamous cell carcinoma in patients with large base of tongue lesions has been challenging. The purpose of the present research is to report prospectively collected speech and swallowing outcomes for patients with disease that encompassed half or more of the base of tongue.Methods:Speech and swallowing data were gathered at 4 evaluation times that spanned the first year of treatment for 32 patients treated with surgery and adjuvant radiation therapy.Results:Speech intelligibility remained above 90% for most patients. In the early postoperative period, patients with 100% of their base of tongue resected experienced decreased intelligibility, which improved by the half‐way mark of the evaluations. The majority of patients resumed normal oral feeding, with those who lacked dentition needing to puree their food. Modified barium swallow studies revealed that only 3 patients aspirated thin liquid at the final evaluation. Five patients used a feeding tube at some point over the year of evaluation. At the final evaluation, only 3 patients required enteral feeding, with 2 of these using the tube in combination with oral feeding and 1 using the tube for primary nutrition.Conclusions:The results of this study suggest that surgical reconstruction of the base of tongue using the radial forearm free flap in patients with large base of tongue lesions can lead to functional speech and swallowing results. © 2007 Wiley Periodicals, Inc. Head Neck, 2007
Work risk‐prevention procedures in highway management and maintenance contracts
Authors: Montes Germán Martínez , Gámez Maria del Carmen Rubio , Romero Juan Carlos Rubio , Mondelo Pedro R. , Terres Fernando 
Published: April 2007

AbstractFollowing the great focus on and investment into roads by European administrations in recent years, a new area of employment has arisen wherein the majority of activities relating to the maintenance and operation of infrastructures have been outsourced. The participation of private companies, the organization of the various contracts and maintenance services, and infrastructure users' increasing demand for better quality services, mean that awareness needs to be raised about the subject of work risk prevention. The authors, having carried out a comparative analysis of Spain and England (countries that represent models in terms of infrastructure management and financing) present the results that were obtained, paying particular attention to the worker–user–safety triad, which determines all the preventative action and measures that are taken. © 2007 Wiley Periodicals, Inc. Hum Factors Man 17: 229–244, 2007.
Toward a hybrid model for usability resource allocation in industrial software product development
Authors: Duffy Colleen M. , Duffy Vincent G. , Yen Benjamin P.C. 
Published: April 2007

AbstractThe organizational aspects of user‐centered software development in a financial services company are presented. The financial services industry sector is one of the industrial sectors to embark on the development of computer software as a consumer product. The nature of business in the service sector predisposes it to encounter difficulties in developing software aimed at meeting customer demands. Lack of familiarity and experience with the product design and implementation processes, as well as reliance on usability for acceptance, are major obstacles encountered. Difficulties, insights, and lessons learned regarding organizational ergonomics issues faced by a user‐centered design group are provided, and a hybrid resource distribution model is proposed to guide other service sector companies in their future software development efforts. © 2007 Wiley Periodicals, Inc. Hum Factors Man 17: 245–262, 2007.
Intracoronary delivery of autologous bone marrow mononuclear cells radiolabeled by 18F‐fluoro‐deoxy‐glucose: Tissue distribution and impact on post‐infarct swine hearts
Authors: Qian Haiyan , Yang Yuejin , Huang Ji , Gao Runlin , Dou Kefei , Yang Guosheng , Li Jianjun , Shen Rui , He Zuoxiang , Lu Minjie , Zhao Shihua 
Published: April 2007

AbstractIntracoronary injection of the bone marrow‐derived mononuclear cells (MNCs) is emerging as a potentially novel therapy for ischemic heart failure. This study was aimed at assessing the efficacy of intracoronary MNC delivery in the myocardium. The in vivo distribution and myocardial homing of intracoronarily delivered MNCs in experimental Chinese swine with acute myocardial infarction (AMI) created by occlusion of left anterior descending (LAD) coronary artery for 90 min. MNCs radiolabeled with 18F‐fluoro‐deoxy‐glucose (18F‐FDG) were delivered using a coronary catheter into the infarct‐related coronary artery 1 week after AMI. Dual‐nuclide single photon emission computed tomography (SPECT) revealed that 1 h after cell infusion, 6.8 ± 1.8% of 18F‐FDG‐labeled MNCs occurred in the infarcted myocardium with the remaining activity found primarily in the liver and spleen. In the heart, MNCs were detected predominantly in the under‐perfused myocardium. The infused cells retained in the hearts at a rate highly correlated with the under‐perfused lesional sizes. Pathological examination further demonstrated that 6 weeks after infusion, compared to controls, the hearts receiving MNCs exhibited less fibrosis and inflammatory infiltrate, more viable tissue, and higher vascular density. Cardiac function was significantly improved in the MNC‐infused hearts. Thus, 18F‐FDG labeling and dual‐nuclide SPECT imaging is capable of monitoring in vivo distribution and homing of MNCs after intracoronary infusion. MNC coronary delivery may improve cardiac function and positive ventricular remodeling in the heart with AMI. J. Cell. Biochem. 102: 64–74, 2007. © 2007 Wiley‐Liss, Inc.
Ascochlorin suppresses oxLDL‐induced MMP‐9 expression by inhibiting the MEK/ERK signaling pathway in human THP‐1 macrophages
Authors: Kang Jeong Han , Kim June‐Ki , Park Won‐Hwan , Park Kwan‐Kyu , Lee Tae‐Sung , Magae Junji , Nakajima Hiroo , Kim Cheorl‐Ho , Chang Young‐Chae 
Published: April 2007

AbstractThe critical initiating event in atherogenesis involves the invasion of monocytes through the endothelial walls of arteries and the transformation of monocytes from macrophages into foam cells. Human THP‐1 monocytic cells can be induced to differentiate into macrophages by phorbol myristate acetate (PMA) and can then be converted into foam cells by exposure to oxidized low‐density lipoprotein (oxLDL). Also, during a chronic inflammatory response, monocytes/macrophages produce the 92‐kDa matrix metalloproteinase‐9 (MMP‐9) that may contribute to the extravasation, migration, and tissue remolding capacities of the phagocytic cells. Here, we investigate the effect of ascochlorin (ASC), a prenylphenol antiviral compound from the fungus Ascochyta viciae, on oxLDL‐induced MMP‐9 expression and activity in human THP‐1 macrophages. ASC reduced oxLDL‐induced MMP‐9 expression and activity in a time‐dependent and dose‐dependent manner. Also, an analysis of MMP‐9 activity using pharmacologic inhibitors showed that ASC inhibits MMP‐9 activity via the extracellular signal‐regulated kinase 1 and kinase 2 pathways. Our results suggest that ASC may be useful as a potent clinical antiatherogenic agent, a topic of considerable interest in the biological chemistry of chemotherapeutic agents. J. Cell. Biochem. 102: 506–514, 2007. © 2007 Wiley‐Liss, Inc.
The transferrin receptor and the tetraspanin web molecules CD9, CD81, and CD9P‐1 are differentially sorted into exosomes after TPA treatment of K562 cells
Authors: Abache Toufik , Le Naour François , Planchon Sébastien , Harper Francis , Boucheix Claude , Rubinstein Eric 
Published: April 2007

AbstractHere we show that treatment of K562 cells with the phorbol ester TPA induces the down‐modulation of various surface antigens. Among them, the transferrin receptor (TfR), the tetraspanin CD81, and a CD81‐associated protein, CD9P‐1, were unique in that their expression levels were lower after 24 h incubation than after 3 h. We demonstrated that like the TfR, CD81 was internalized at early times, and was less synthesized at latter times. Despite the association of a fraction of the TfR with CD81, these two molecules were subjected to different fates. TPA increased targeting of CD81 and CD9P‐1 into exosomes but strongly reduced the localization of the TfR in these vesicles. Using this model we have shown that a fraction of CD81 and CD9P‐1 in exosomes comes from a surface pool and that these molecules remain associated in exosomes. However, CD9P‐1 could be targeted to exosomes in the absence of CD81 and of another tetraspanin, CD9. The targeting of CD9 into exosomes did not require palmitoylation of the protein. J. Cell. Biochem. 102: 650–664, 2007. © 2007 Wiley‐Liss, Inc.
Orthogonal natural atomic orbitals form an appropriate one‐electron basis for expanding CASSCF wave functions into localized bonding schemes and their weights
Authors: Bachler Vinzenz 
Published: April 2007

AbstractLocalized bonding schemes and their weights have been obtained for the π‐electron system of nitrone by expanding complete active space self‐consistent field wave functions into a set of Slater determinants composed of orthogonal natural atomic orbitals (NAOs) of Weinhold and Landis (Valency and Bonding: A Natural Bond Orbital Donor‐Acceptor Perspective, 2005). Thus, the derived bonding schemes are close to orthogonal valence bond structures. The calculated sequence of bonding scheme weights accords with the sequence of genuine resonance structure weights derived previously by Ohanessian and Hiberty (Chem Phys Lett 1987, 137, 437), who employed nonorthogonal atomic orbitals. This accord supports the notion that NAOs form an appropriate orthogonal one‐electron basis for expanding complete active space self‐consistent field wave functions into meaningful bonding schemes and their weights. © 2007 Wiley Periodicals, Inc. J Comput Chem, 2007
The corrosion behaviour of the aluminum alloy 7075/SiCp metal matrix composite prepared by spray deposition
Authors: Cheng Y.L. , Chen Z.H. , Wu H.L. , Wang H.M. 
Published: April 2007

AbstractAluminum alloy 7075 and 7075/SiCp (MMC) were prepared by multi‐layer spray deposition method and the corrosion behaviour of them were studied by electrochemical measurements to study the effect of the addition of silicon carbide on the corrosion behaviour of the MMC. The electrochemical noise result shows that the amplitude of the potential noise of the composite is lower than that of the spray deposited 7075 alloy. The potentiodynamic polarization curves results show that both the cathodic oxygen reduction current density and the anodic dissolution current density of the 7075/SiCp MMC are less than those of the 7075 alloy. Thus, the addition of SiC particles increases the corrosion resistance of the MMC. This may be due to that the microstructure of the spray deposited MMC is compact and SiC particles are nonmetallic material, the addition of it minimizes the real corrosion area of the alloy.
High temperature alloy chloridation at 850°C. Part I: Comparison of Ni‐based and Fe‐based alloy behaviour
Authors: Chevalier S. , Ched'Homme S. , Bekaddour A. , Amilain‐Basset K. , Buisson L. 
Published: April 2007

AbstractEight alloys were tested under Ar/Cl2 atmosphere at 850°C for 15 min and 1 h. Their gross and net weights were evaluated together with the base metal consumption. Macroscopic and microscopic micrographs, associated with chemical analyses and X‐ray diffraction gave the composition and microstructure of the corrosion products. Huge differences were observed if one compared the nickel based alloy behaviour to that of the iron based alloy. Molybdenum and tungsten could also play a role, but it was not clearly defined until now. A tentative evaluation of the best candidates will be given, according the experimental conditions of this work and the chosen criteria. A corrosion index was established after each observation and helped to classify the different material behaviour under chloridation. The Ni‐based alloys possessed the best chloridation behaviour in the present conditions, followed by the Ni‐based alloys containing more Cr and finally by the Fe‐based materials. The chloridation behaviours were discussed according to the volatility of the chlorides species formed on the different alloys.
Biomarker discovery in lung cancer—promises and challenges of clinical proteomics
Authors: Bharti Ajit , Ma Patrick C. , Salgia Ravi 
Published: April 2007

AbstractLung cancer is a devastating illness with an overall poor prognosis. To effectively address this disease, early detection and novel therapeutics are required. Early detection of lung cancer is challenging, in part because of the lack of adequate tumor biomarkers. The goal of this review is to summarize the knowledge of current biomarkers in lung cancer, with a focus on important serum biomarkers. The current knowledge on the known serum cytokines and tumor biomarkers of lung cancer will be presented. Emerging trends and new findings in the search for novel diagnostic and therapeutic tumor biomarkers using proteomics technologies and platforms are emphasized, including recent advances in mass spectrometry to facilitate tumor biomarker discovery program in lung cancer. It is our hope that validation of these new research platforms and technologies will result in improved early detection, prognostication, and finally the treatment of lung cancer with potential novel molecularly targeted therapeutics. © 2007 Wiley Periodicals, Inc., Mass Spec Rev
Continuous dependence on the geometry and on the initial time for a class of parabolic problems I
Authors: Payne L. E. , Philippin G. A. , Proytcheva V. 
Published: April 2007

AbstractIn this paper, we investigate the continuous dependence on the geometry and the initial time for solutions u(x, t) of a class of nonlinear parabolic initial‐boundary value problems. Copyright © 2007 John Wiley & Sons, Ltd.
Morphology and thermal properties of a PC/PE blend with reactive compatibilization
Authors: Yin Bo , Zhao Yin , Pan Min‐min , Yang Ming‐bo 
Published: April 2007

AbstractReactive compatibilization of immiscible polymers is becoming increasingly important and hence a representative study of a polycarbonate/high density polyethylene (PC/HDPE) system is the focus of this paper. A grafted copolymer PC‐graft‐ethylene‐co‐acrylic acid (PC‐graft‐EAA) was generated as a compatibilizer in situ during processing operation by ester and acid reaction between PC and ethylene‐acrylic acid (EAA) in the presence of the catalyst dibutyl tin oxide (DBTO). As the polyethylene (PE) matrix does not play any part during the synthesis of the copolymer and since PC and EAA are also immiscible, to simplify the system, the influence of this copolymer formation at the interface between PC and EAA on rheological properties, phase morphology, and crystallization behavior for EAA/PC binary blends was first studied. The equilibrium torque increased with the DBTO content increasing in EAA/PC blends on Haake torque rheometer, indicating the in situ formation of the graft copolymer. Scanning electron microscopy (SEM) studies of cryogenically fractured surfaces showed a significant change at the distribution and dispersion of the dispersed phase in the presence of DBTO, compared with the EAA/PC blend without the catalyst. Differential scanning calorimetry (DSC) studies suggested that the heat of fusion of the EAA phase in PC/EAA blends with or without DBTO reduced with the formation of the copolymer compared with pure EAA. Then morphological studies and crystallization behavior of the uncompatibilized and compatibilized blends of PC/PE were studied as functions of EAA phase concentration and DBTO content. Morphological observations in PC/PE blends also revealed that on increasing the EAA content or adding the catalyst DBTO, the number of microvoids was reduced and the interface was intensive as compared to the uncompatibilized PC/PE blends. Crystallization studies indicated that PE crystallized at its bulk crystallization temperature. The degree of crystallinity of PE phase in PC/PE/EAA blends was also reduced with the addition of EAA and DBTO compared to the uncompatibilized blends of PC/PE, indicating the decrease in the degree of crystallinity was more in the presence of PC‐graft‐EAA. Copyright © 2007 John Wiley & Sons, Ltd.
Hepatocyte growth factor and c‐Met expression in pericytes: implications for atherosclerotic plaque development
Authors: Liu Y , Wilkinson FL , Kirton JP , Jeziorska M , Iizasa H , Sai Y , Nakashima E , Heagerty AM , Canfield AE , Alexander MY 
Published: April 2007

AbstractIntraplaque neovascularization contributes to the progression of atherosclerosis. Our aim is to understand the mobilization of cells and factors involved in this process. We investigated the localization of hepatocyte growth factor (HGF) and its receptor, c‐Met, in human atherosclerotic plaques, together with the effects of HGF on pericyte migration in vitro. Atherosclerotic femoral arterial segments were collected and analysed from 13 subjects who were undergoing lower limb amputation. Pericytes were identified in human lesions using a 3G5 antibody. Immunohistochemical analysis localized HGF mainly around microvessels, in association with some, but not all, CD31‐positive endothelial cells. c‐Met expression was mainly associated with smooth muscle cells and pericytes, around some, but not all, microvessels within the atherosclerotic lesions; no detection was apparent in normal internal mammary arteries. Using RT–PCR, we demonstrated expression of HGF and c‐Met in a rat pericyte cell‐line, TR–PCT1, and in primary pericytes. HGF treatment of TR‐PCT1 cells induced their migration, but not their proliferation, in a dose‐dependent manner (10–100 ng/ml, p < 0.01), an effect mediated by activation of the serine/threonine kinase Akt, shown by western blot analysis. Treating the cells with the PI3K inhibitors Wortmannin (0.1 µM) or LY294002 (10 µM) abolished these effects. This work demonstrates the expression of c‐Met and HGF in human atherosclerotic arteries, in association with SM‐actin‐positive cells and CD‐31‐positive cells, respectively. HGF induces pericyte migration via PI3‐kinase and Akt activation in vitro. HGF and c‐Met may be involved in neovascularization during plaque development, and may recruit pericytes to neovessels. Since pericytes are thought to mechanically stabilize new blood vessels, these factors may function to protect against haemorrhage. Copyright © 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Seasonal variations in the isoflavonoids of Radix Puerariae
Authors: Sibao Chen , Dajian Yang , Shilin Chen , Hongxi Xu , Chan Albert S. C. 
Published: April 2007

AbstractThe variations in isoflavonoid content in Radix Puerariae, the root of Pueraria lobata (Wild) Ohwi, have been examined in order to determine the optimum time to harvest the herb. Total isoflavonoid contents have been analysed by a UV spectrophotometric method whilst HPLC with photodiode array detection has been used to monitor the contents of seven major individual isoflavonoids, namely, 3′‐hydroxypuerarin, puerarin, 3′‐methoxypuerarin, daidzin, genistin, formononetin‐7‐glucoside and daidzein. Ninety‐six samples of Radix Puerariae of different ages and harvested in different months were collected and analysed. The results clearly showed that 3‐year‐old roots harvested in January have the highest yields of isoflavonoid compounds. Moreover, the results obtained by both methods have a strong correlation. This suggested that the simple and fast UV spectrophotometric method could be used for monitoring the phytochemical quality of Radix Puerariae. Copyright © 2007 John Wiley & Sons, Ltd.
Separation and quantification of the cellular thiol pool of pea plants treated with heat, salt and atrazine
Authors: Ivanov Sergei Veselinov , Kerchev Pavel Ivanov 
Published: April 2007

AbstractA novel procedure for the separation of the cellular thiol pool according to the molecular weight and localization of compounds with sulphydryl groups is presented. This simple and rapid method allows the differentiation of thiols into three major fractions—low molecular weight (LMT, primarily glutathione and free cysteine), protein‐bound (TPT) and pellet‐bound (PBT, associated with cell walls and broken organelles). Moreover, determination of the ratio between surface (readily reactive) thiols (ATG) and those that are more or less buried in the protein structure (BTG) can be achieved. In intact pea leaves, the amounts of the total thiols (LMT + PBT + TPT) varies from 2.5 to 4.8 µmol/g of fresh material. The data for LMT, PBT and TPT were related to each other in the approximate ratio 1:2:7. Treatments of pea plants with high temperature, salinity and low amounts of atrazine affect these sulphydryl types differently. For a greater understanding of the applicability of this method to physiological research, the main mechanisms leading to alterations in the cellular thiol pool are discussed. Furthermore, it is suggested that the proportion of available to buried thiols (ATG/BTG) in proteins could be used as a convenient marker for stress impacts. Copyright © 2007 John Wiley & Sons, Ltd.
Dithiothreitol decreases in vitro activity of ADP‐glucose pyrophosphorylase from leaves of apple (Malus domestica Borkh.) and many other plant species
Authors: Chen Li‐Song , Qi Yi‐Ping 
Published: April 2007

AbstractInclusion of dithiothreitol (DTT) in the extraction buffer and pre‐incubation of apple leaf ADP‐glucose pyrophosphorylase (AGPase) with DTT resulted in a decrease in AGPase activity whether the assay was performed in the presence or absence of 3‐phosphoglycerate (PGA). When PGA was included in the pre‐incubation mixture or when pre‐incubation of AGPase with PGA was followed by DTT, the latter did not cause any decrease in AGPase activity. However, once AGPase was decreased by DTT, subsequent incubation of the enzyme with PGA did not reverse the decrease. Pre‐incubation of AGPase from leaves of Arabidopsis thaliana, sorghum, soybean, tobacco, spinach, wheat, barley, tomato and potato, and tubers of potato with DTT, generally caused a decrease in AGPase activity when assayed in the presence of PGA. When assayed in the absence of PGA, however, a diverse response of AGPase was observed among species to pre‐incubation with DTT. The activity of AGPase from potato tubers was increased by DTT; the activity of AGPase from both potato and tomato leaves was not affected by DTT; the activity of AGPase from leaves of other species was decreased by DTT. It is concluded that DTT decreases in vitro activity of AGPase from leaves of apple and many other plant species such that DTT should not be routinely included in the extraction or assay mixture of leaf AGPase. Copyright © 2007 John Wiley & Sons, Ltd.