bluuebunny/crossref_metadata_2025_split huggingface dataset:
Failure Rate of Meniscal Repair With ACL Reconstruction Among Professional Athletes: A Study of 196 Patients From the SANTI Study Group With a Mean Follow-up of 96 Months
Authors: An Jae-Sung , Lahsika Mohammed , Nlandu Alice , Giurazza Giancarlo , Chamoux Julien , Uso Marc Barrera , Cardarelli Silvia , Vieira Thais Dutra , Sonnery-Cottet Bertrand 
Published: February 2025

Background: There is still controversy regarding the ideal management method for meniscal tears combined with anterior cruciate ligament (ACL) injury, especially for professional athletes. Purposes: To (1) describe the incidence of secondary meniscectomy in professional athletes after undergoing meniscal repair concomitant to primary ACL reconstruction (ACLR) and (2) identify the associated risk factors for repair failure of the medial meniscus (MM) and/or lateral meniscus (LM) in this population at long-term follow-up. Study Design: Cohort study; Level of evidence, 3. Methods: This was a retrospective comparative study. Included were professional athletes who underwent arthroscopic primary ACLR and repair of MM and/or LM tears between January 2013 and December 2022and who had ≥2 years of follow-up. Risk factors associated with secondary meniscectomy were analyzed using a Cox proportional hazards model. Results: Out of 230 professional athletes (mean ± SD age, 23.3 ± 5.0 years; mean Tegner score, 9.3 ± 1.0) who underwent primary ACLR, 196 patients (85.2%) also had meniscal repair. The most common type of graft used was hamstring tendon (74%), and 74% of patients underwent a concomitant lateral extra-articular procedure. Among the 196 patients with meniscal lesions, 37% had LM lesions, 29% had MM lesions, and 34% had both LM and MM lesions. LM tears were repaired in 92.8% of cases, while 7.2% were left in situ. The most common type of repair for LM tears was the all-inside technique. MM tears were repaired in 97.6% of cases, with 2.4% left alone. The most common type of repair for MM tears was the suture hook technique, and no meniscectomies were performed. At a mean follow-up of 95.8 ± 45.1 months, 26 patients (13.3%) underwent a secondary meniscectomy: 7 (5.0%) for LM and 19 (15.4%) for MM. A Cox model revealed no significant risk factors associated with secondary lateral or medial meniscectomy. Conclusion: At long-term follow-up, the meniscal repair failure rate in this population of patients who underwent primary ACLR was 13.2% overall, 5% for LM tears and 15.4% for MM tears. No risk factors for secondary meniscectomy were found.
Arthroscopic Anchor Placement During Shoulder Anterior Labral Repair in the Beach-Chair Versus the Lateral Decubitus Position: An Imaging Analysis
Authors: Jones Steven D. , Lencioni Alex , Elrick Bryant P. , Baldini Todd , Bravman Jonathan T. , McCarty Eric C. , Seidl Adam , Frank Rachel M. 
Published: February 2025

Background: Arthroscopic shoulder capsuloligamentous repair can be performed in the beach-chair (BC) or lateral decubitus (LD) position. Patient positioning may influence anchor placement. Limited research exists evaluating these 2 positioning techniques, specifically with respect to anchor position and trajectory. Purpose/Hypothesis: The purpose of this study was to perform a cadaveric imaging analysis of arthroscopic anterior shoulder stabilization in the LD and BC positions. It was hypothesized that repairs performed in the LD position would demonstrate more accurate anterior inferior anchor positioning. Study Design: Controlled laboratory study. Methods: Nine matched pairs of cadaveric shoulders (18 total shoulders) were used. Specimens underwent arthroscopic anterior inferior labral repair using 3 suture anchors placed in the 3:30, 4:30, and 5:30 positions. Repairs were performed by 2 fellowship-trained orthopaedic surgeons each trained in a respective technique. Specimens then underwent computed tomography analysis to evaluate anchor position and trajectories. Target anchor location was determined using a 3-dimensional computed tomography glenoid model, using ImageJ software, with the clockface overlaid onto the glenoid. The 3-o’clock position was set at 0° for reference, with the 3:30, 4:30, and 5:30 anchor positions set at −15°, −45°, and −75°, respectively. One-tailed t tests were used to compare anchor position deviation with target ideals. Results: Inferior anchor (5:30) placement was found to be more accurate in the LD position, while superior anchor (3:30) placement was found to be more accurate in the BC position. Specifically, the LD group did not demonstrate statistically significant deviation from the target ideal (−75°) for the 5:30 anchor. The true deviance for the LD group from the 5:30 target angle was between −78.6° and −58.5° ( P = .178), while the BC group did not demonstrate statistically significant deviation for the target ideal (−15°) for the 3:30 anchor. The true deviance from the target angle was between −37.9° and 1.79° ( P = .732). Both groups demonstrated adequate anchor trajectories. Conclusion: Both positioning techniques remain safe and efficacious. The LD position may offer improved inferior anchor placement, and the BC position may offer improved superior anchor placement. Additional studies are warranted to further elucidate the advantages and disadvantages of each position. Clinical Relevance: Knowledge pertaining to the accuracy of anchor placement based on patient positioning and anchor location may aid surgeons in choosing operating positions to potentially optimize anchor placement during arthroscopic anterior shoulder stabilization surgery.
Biomechanical Risk Factors for Increased Anterior Cruciate Ligament Loading and Injury: A Systematic Review
Authors: Belkhelladi Malachy , Cierson Tanya , Martineau Paul A. 
Published: February 2025

Background: Understanding the biomechanical risk factors for noncontact anterior cruciate ligament (ACL) injury can inform machine learning models, aid in prevention strategies, and guide rehabilitation protocols, reducing the incidence and burden of these injuries in both athletes and the general population. Purpose: To determine the biomechanical risk factors associated with noncontact ACL injury and increased knee loading. Study Design: Systematic review; Level of evidence, 4. Methods: A literature search was conducted according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Randomized, cohort, case-control, and cross-sectional studies identifying noncontact biomechanical risk factors for ACL injuries published before May 2023 were included in this review. Excluded were studies focused on contact ACL injuries, those focused on biomechanical risk factors postinjury, and those not published in the English language. The authors highlighted biomechanical risk factors not extensively covered in previous reviews, including the toe-in position, increased contralateral pelvic hike, increased hip internal rotation angle, and specific ankle angles. A quantitative overview of the included studies was conducted, highlighting the frequency of each biomechanical factor reported as potentially related to ACL injury or loading risk. Results: A total of 28 studies (2819 athletes) were selected for analysis. The majority of these studies (22/28) were cross-sectional, primarily assessing ACL load indirectly via knee valgus moment or ground-reaction forces, while case-control and cohort studies focused on ACL injury incidence. Overall, 83% (5/6) of the studies assessing upper body biomechanics found that trunk flexion/extension and perturbations affect ACL loading risk. Of studies assessing hip biomechanics, 83% (10/12) showed increased ACL loading or injury risk with increased hip abduction/internal rotation angles. For the foot and ankle, increased toe-in/toe-out landing in 67% of studies (2/3) demonstrated higher stress on the ACL. Knee biomechanics were associated with increased ACL loading in 100% of the respective studies (5/5), with decreased knee flexion angles leading to increased loading. Conclusion: The data demonstrated that factors associated with increased medial knee alignment, sagittal alignment of the trunk, and decreased lateral trunk flexion reduced both knee loading and ACL injury risk. Targeted prevention and detection strategies addressing high-risk biomechanics may reduce injury incidence, underscoring the need for further research to optimize intervention programs.
Mental health and the COVID-19 pandemic in Europe: A focus on the self-employed
Authors: Gerogiannis Ilias , Capecchi Stefania , Curtarelli Maurizio 
Published: February 2025

Self-employed workers worldwide encountered significant mental health challenges during the COVID-19 pandemic. Nevertheless, it remains unclear whether the pandemic has affected the mental health of the self-employed more significantly than employees. To address this question, we analyzed data from more than 4,000 self-employed individuals across the 27 European Union (EU) member states. We found that the pandemic had a more significant impact on the mental health of the self-employed in the EU, especially those working in the health and education sectors and countries with the highest stringency measures. Our findings also reveal that the pandemic has prompted self-employed individuals, particularly self-employed women, to be more open to talking about their mental health challenges at work, suggesting a shift in their perception of mental health stigma. This study highlights an opportunity and a need for EU policymakers to introduce targeted measures to support the self-employed in building back better in the post-COVID-19 world. JEL CLASSIFICATIONS: I31, L26
Oral Microbiome and Dental Caries in Kenyan Children and Adolescents Living with HIV
Authors: Wang Y. , Matangkasombut O. , Kemoli A.M. , John-Stewart G. , Benki-Nugent S. , Slyker J. , Aldrovandi G.M. , Seminario A.L. 
Published: February 2025

Objective: This cross-sectional study evaluates the association between the oral microbiome and the presence of untreated dental caries among Kenyan children and adolescents living with HIV (CALHIV). Methods: We collected 83 oral swab samples from CALHIV receiving medical care at Kenyatta National Hospital. We used the 16S rRNA sequencing technique on oral swab samples to profile bacterial composition. Only CALHIV with results of oral microbiome and dental examinations simultaneously were included in the further analysis. We compared the alpha and beta diversity of the oral microbiome between individuals with and without dental caries. Log-ratio LASSO regression with 2-stage estimation, 10-fold cross-validation, and adjustment for the false discovery rate was applied to select the best features that can predict caries status at the phylum level. Results: Of the 70 participants with both oral health data and oral microbiome data available, 66% had dental caries. The group with dental caries had higher Chao 1 alpha diversity (P = 0.046) but not beta diversity. Log-ratio LASSO regression had a higher ratio of paired features proteobacteria/SR1 (P = 0.0067), Euryarchaeota/SR1 (P = 0.0074), Acidobacteria/SR1 (P = 0.0079), and Fusobacteria/SR1 (P = 0.0125) at the phylum level and was significantly associated with a lower prevalence of untreated dental caries. A higher abundance of SR1 together with certain bacteria was associated with a higher prevalence of untreated dental caries. Conclusions: This is the first study to analyze the oral microbiome of CALHIV and untreated dental caries in a cohort that had received medical care since their first years of life. While this study provides information about the paired feature selection for dental caries in CALHIV on ART using cross-validation, SR1 may interact with other bacteria to increase the prevalence of untreated dental caries. Longitudinal microbiome data and samples from multiple sites in the oral cavity will be essential for understanding and confirming these findings. Knowledge Transfer Statement: The results of this study can help clinicians understand how SR1 interacts with other bacteria that may increase the prevalence of untreated dental caries in children and adolescents living with HIV and on long-term antiretroviral therapy. Furthermore, these findings may provide valuable insights for future research, informing longitudinal microbiome data and sampling from various sites in the oral cavity.
Roxadustat improves diabetic myocardial injury by upregulating HIF-1α/UCP2 against oxidative stress
Authors: Fang Tingting , Ma Congcong , Yang Bingyun , Zhao Meiyu , Sun Luning , Zheng Ningning 
Published: February 2025

Abstract

Background
Diabetes mellitus (DM), characterized by hyperglycemia, is intricately linked with cardiovascular complications. Hyperglycemia induces oxidative stress, compromising mitochondria energy metabolism disturbances, leading to cardiomyocyte hypoxia and dysregulation of hypoxia-inducible factor-1α (HIF-1α), thereby exacerbating diabetic myocardial injury. Roxadustat (FG-4592), as an inhibitor of HIF-PHD, reduces HIF-1α degradation and regulates the transcription and function of downstream target genes. This study explores the protective effect of FG-4592 on the diabetic myocardium and further investigates the specific mechanisms responsible for this action.

Methods
We established diabetic myocardial injury mice and high glucose-induced rat cardiomyocyte models, administered FG-4592 pretreatment to clarify the protective effects and related mechanisms of FG-4592 on diabetic myocardial injury by detecting changes in oxidative stress, mitochondrial function, and related pathways.

Results
FG-4592 demonstrated cardioprotective effects in diabetic mice by regulating mitochondrial structure and function, as well as maintaining oxidative stress balance in the myocardium. It stabilized HIF-1α, activated UCP2, and enhanced the PI3K/AKT/Nrf2 pathway, reducing mitochondrial superoxide production, improving mitochondrial respiratory potential, and modulating oxidative stress markers in high glucose-induced cardiomyocytes.

Conclusions
FG-4592 exerts protective effects against diabetic myocardial injury by reducing oxidative stress. The mechanism is linked with the upregulation of HIF-1α and UCP2, which subsequently activate the PI3K/AKT/Nrf2 signaling pathway.
Nano-XRF of lung fibrotic tissue reveals unexplored Ca, Zn, S and Fe metabolism: a novel approach to chronic lung diseases
Authors: Falcones Bryan , Kahnt Maik , Johansson Ulf , Svobodová Barbora , von Wachenfelt Karin A. , Brunmark Charlott , Dellgren Göran , Elowsson Linda , Thånell Karina , Westergren-Thorsson Gunilla 
Published: February 2025

Abstract
Synchrotron-radiation nano-X-Ray Fluorescence (XRF) is a cutting-edge technique offering high-resolution insights into the elemental composition of biological tissues, shedding light on metabolic processes and element localization within cellular structures. In the context of Idiopathic Pulmonary Fibrosis (IPF), a debilitating lung condition associated with respiratory complications and reduced life expectancy, nano-XRF presents a promising avenue for understanding the disease’s intricate pathology. Our developed workflow enables the assessment of elemental composition in both human and rodent fibrotic tissues, providing insights on the interplay between cellular compartments in chronic lung diseases. Our findings demonstrate trace element accumulations associated with anthracosis, a feature observed in IPF. Notably, Zn and Ca clusters approximately 750 nm in size were identified exclusively in IPF samples. While their specific role remains unclear, their presence may be associated with disease-specific processes. Additionally, we observed Fe and S signal colocalization in 650-nm structures within some IPF cells. Fe-S complexes in mitochondria are known to be associated with increased ROS production, suggesting a potential connection to the disease pathology. In contrast, a bleomycin-induced fibrosis rodent model exhibits a different elemental phenotype with low Fe and increased S, Zn, and Ca. Overall, our workflow highlights the effectiveness of synchrotron-based nano-XRF mapping in analyzing the spatial distribution of trace elements within diseased tissue, offering valuable insights into the elemental aspects of IPF and related chronic lung diseases.
Preoperative liquid biopsy transcriptomic panel for risk assessment of lymph node metastasis in T1 gastric cancer
Authors: Ding Ping’an , Wu Jiaxiang , Wu Haotian , Ma Wenqian , Li Tongkun , Yang Peigang , Guo Honghai , Tian Yuan , Yang Jiaxuan , Er Limian , Gu Renjun , Zhang Lilong , Meng Ning , Li Xiaolong , Guo Zhenjiang , Meng Lingjiao , Zhao Qun 
Published: February 2025

Abstract

Background
The increasing incidence of early-stage T1 gastric cancer (GC) underscores the need for accurate preoperative risk stratification of lymph node metastasis (LNM). Current pathological assessments often misclassify patients, leading to unnecessary radical surgeries.

Methods
Through analysis of transcriptomic data from public databases and T1 GC tissues, we identified a 4-mRNA panel (SDS, TESMIN, NEB, and GRB14). We developed and validated a Risk Stratification Assessment (RSA) model combining this panel with clinical features using surgical specimens (training cohort: n = 218; validation cohort: n = 186), gastroscopic biopsies (n = 122), and liquid biopsies (training cohort: n = 147; validation cohort: n = 168).

Results
The RSA model demonstrated excellent predictive accuracy for LNM in surgical specimens (training AUC = 0.890, validation AUC = 0.878), gastroscopic biopsies (AUC = 0.928), and liquid biopsies (training AUC = 0.873, validation AUC = 0.852). This model significantly reduced overtreatment rates from 83.9 to 44.1% in tissue specimens and from 84.4 to 56.0% in liquid biopsies. The 4-mRNA panel showed specificity for T1 GC compared to other gastrointestinal cancers (P < 0.001).

Conclusions
We developed and validated a novel liquid biopsy-based RSA model that accurately predicts LNM in T1 GC patients. This non-invasive approach could significantly reduce unnecessary surgical interventions and optimize treatment strategies for high-risk T1 GC patients.
Acute kidney injury and outcomes in hospitalized children with autoimmune rheumatic disease
Authors: Lin Chien-Hung , Liu Wen-Sheng , Wan Chuan , Wang Hsin-Hui 
Published: February 2025

Abstract

Background
Autoimmune rheumatic diseases (ARDs) in children can negatively impact renal function, potentially leading to acute kidney injury (AKI). This study compares the prevalence of AKI and other adverse in-hospital outcomes among hospitalized children with ARDs.

Methods
A retrospective analysis was conducted using the United States Nationwide Inpatient Sample (NIS) database from 2005 to 2020. The study included children aged 1–17 years with ARDs, categorized into inflammatory arthritis, ANCA-associated vasculitis, systemic lupus erythematosus (SLE), systemic sclerosis (SSc), and other connective tissue diseases. Logistic regression assessed associations between ARD types and outcomes, including AKI, dialysis, and major adverse events.

Results
Among 13,891 children with ARDs, 8.2% developed AKI and 1.3% required dialysis. Compared to inflammatory arthritis, ANCA-associated vasculitis significantly increased the risk of AKI (aOR = 11.20, 95% CI: 8.08–15.51) and dialysis (aOR = 40.60, 95% CI: 13.54-121.71). SLE also elevated risks of AKI (aOR = 4.16, 95% CI: 3.20–5.40) and dialysis (aOR = 11.34, 95% CI: 4.15–31.01). Children with SSc had increased risks of infection/pneumonia (aOR = 2.51, 95% CI: 1.84–3.41) and sepsis (aOR = 2.13, 95% CI: 1.26–3.58).

Conclusions
Children with ARDs, especially those with ANCA-associated vasculitis and SLE, face elevated risks of AKI and dialysis. These findings underscore the importance of vigilant monitoring and tailored management in this population.
Identification of Houge type of X-linked syndromic mental retardation caused by CNKSR2 truncated variants
Authors: Chang Si-Hua , Jin Jie-Yuan , Hu Yi-Qiao , Wang Run-Yan , Xiang Rong , Wang Xia 
Published: February 2025

Abstract

Background
Houge type of X-linked syndromic mental retardation (MRXSHG) is a type of X-linked condition which is mainly manifested as delayed development, mental retardation, epilepsy that begins at an early age, and delayed language acquisition. MRXSHG is a serious disorder with CNKSR2 variant and at least 34 variants have been identified in MRXSHG patients. However, the genotype-phenotype correlation and variants characteristics of CNKSR2 need further investigation and improvement.

Methods
Two Chinese MRXSHG families were recruited, and their genetic causes were investigated using whole-exome sequencing (WES), Sanger sequencing, and bioinformatics analysis. To verify the impact of these variants, we used real-time PCR and minigenes consisting of exon 14, intron 14, and exon 15 from both the wild-type and the c.1658-3_1676del DNA sequences.

Results
In this study, we reported two Chinese boys with MRXSHG and described some rare MRXSHG phenotypes, such as delayed bone age, slightly widened right fissure, and an underdeveloped right temporal lobe, characterized by reduced growth and volume compared to typical development. Two novel variants in CNKSR2 (c.1658-3_1676del and c.1102G > T, p.Gly368*) were identified in these cases. Minigenes results revealed that variant c.1658-3_1676del produced an aberrant spliceosome assembly.

Conclusions
We identified two novel CNKSR2 variants in MRXSHG families, expanding the variant spectrum of CNKSR2, enriching MRXSHG-related phenotypes, and contributing to genetic counseling for MRXSHG patients.
Manufacturing and characterization of conductive threads based on twisting process for applying smartwear
Authors: Kim Hyelim , Rho Soohyeon , Jeong Wonyoung 
Published: February 2025

Abstract
A basic study was conducted to manufacture conductive composite threads for use in textile-type electrodes and circuits for smartwear that can be sewn, embroidered, and knitted. The two-types of poly(ethylene terephthalate(PET) filament with a circular cross-section(SD), and an oval cross-section and curved surface(DT), and the conductive yarn was selected as a silver-coated polyamide(AP). The samples were produced two-ply PET yarn as the first twist, then using AP as the second twist, and both processes were applied with 100, 200, 500, and 1,000 TPM. As the number of twists increased, the packing density of samples increased, and the bulkiness of DT was decreased. In addition, the elongation at break tended to increase as the number of twists increased, especially for samples manufactured with DT with a curved surface appeared larger. The linear resistance results increased as the number of second twists in which the conductive yarn was twisted increased. It was confirmed that the area of AP exposed to the outside was more prominent in SD-AP, resulting in decreased linear resistance. Based on the results, samples that have the excellent mechanical and electrical performance were selected to manufacture textile-type electrodes that embedded leg sleeves to measure the surface electromyography(sEMG) signal collection performance. The signal-to-noise ratios(SNR) of textile-type electrode manufactured by SD - AP and DT - AP were 4.54 and 14.96, respectively, confirming the possibility of collecting EMG signals. Thus, this study is expected to be applicable to a wider range of fields than smartwear.